LOCUS       DAZFKM010000001       103928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFKM010000001 DAZFKM010000000
VERSION     DAZFKM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 103928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..764
                     /locus_tag="ACHKAY_000001"
     CDS             48..764
                     /locus_tag="ACHKAY_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5698758.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            794..1294
                     /locus_tag="ACHKAY_000002"
     CDS             794..1294
                     /locus_tag="ACHKAY_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO5698759.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1386..4070
                     /locus_tag="ACHKAY_000003"
     CDS             1386..4070
                     /locus_tag="ACHKAY_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO5698760.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4067..5141
                     /locus_tag="ACHKAY_000004"
                     /pseudo
     CDS             4067..5141
                     /locus_tag="ACHKAY_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5229..6527)
                     /gene="exuT"
                     /locus_tag="ACHKAY_000005"
     CDS             complement(5229..6527)
                     /gene="exuT"
                     /locus_tag="ACHKAY_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO5698761.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7010..8422
                     /gene="uxaC"
                     /locus_tag="ACHKAY_000006"
     CDS             7010..8422
                     /gene="uxaC"
                     /locus_tag="ACHKAY_000006"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO5698762.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8437..9924
                     /gene="uxaA"
                     /locus_tag="ACHKAY_000007"
     CDS             8437..9924
                     /gene="uxaA"
                     /locus_tag="ACHKAY_000007"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO5698763.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10007..10558
                     /gene="ygjV"
                     /locus_tag="ACHKAY_000008"
     CDS             10007..10558
                     /gene="ygjV"
                     /locus_tag="ACHKAY_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO5698764.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10563..11807)
                     /gene="sstT"
                     /locus_tag="ACHKAY_000009"
     CDS             complement(10563..11807)
                     /gene="sstT"
                     /locus_tag="ACHKAY_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO5698765.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12206..13171)
                     /gene="alx"
                     /locus_tag="ACHKAY_000010"
     CDS             complement(12206..13171)
                     /gene="alx"
                     /locus_tag="ACHKAY_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO5698766.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13454..14440)
                     /gene="ygjR"
                     /locus_tag="ACHKAY_000011"
     CDS             complement(13454..14440)
                     /gene="ygjR"
                     /locus_tag="ACHKAY_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5698767.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14519..15198)
                     /gene="ygjQ"
                     /locus_tag="ACHKAY_000012"
                     /pseudo
     CDS             complement(14519..15198)
                     /gene="ygjQ"
                     /locus_tag="ACHKAY_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15275..15778)
                     /gene="ygjP"
                     /locus_tag="ACHKAY_000013"
     CDS             complement(15275..15778)
                     /gene="ygjP"
                     /locus_tag="ACHKAY_000013"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO5698768.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15863..16999
                     /gene="rlmG"
                     /locus_tag="ACHKAY_000014"
     CDS             15863..16999
                     /gene="rlmG"
                     /locus_tag="ACHKAY_000014"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO5698769.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17190..17273
                     /locus_tag="ACHKAY_000015"
                     /pseudo
     CDS             17190..17273
                     /locus_tag="ACHKAY_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17270..17686
                     /gene="higA"
                     /locus_tag="ACHKAY_000016"
     CDS             17270..17686
                     /gene="higA"
                     /locus_tag="ACHKAY_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO5698770.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17731..19482)
                     /gene="fadH"
                     /locus_tag="ACHKAY_000017"
                     /pseudo
     CDS             complement(17731..19482)
                     /gene="fadH"
                     /locus_tag="ACHKAY_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
     gene            complement(19587..20324)
                     /locus_tag="ACHKAY_000018"
     CDS             complement(19587..20324)
                     /locus_tag="ACHKAY_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO5698771.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWMHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRSAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVHPYPKPQTVKTGLKESE"
     gene            complement(20278..20478)
                     /locus_tag="ACHKAY_000019"
     CDS             complement(20278..20478)
                     /locus_tag="ACHKAY_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5698772.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            20593..21055
                     /locus_tag="ACHKAY_000020"
                     /pseudo
     CDS             20593..21055
                     /locus_tag="ACHKAY_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21094..21339)
                     /locus_tag="ACHKAY_000021"
     CDS             complement(21094..21339)
                     /locus_tag="ACHKAY_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5698773.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
     gene            complement(21375..21554)
                     /locus_tag="ACHKAY_000022"
     CDS             complement(21375..21554)
                     /locus_tag="ACHKAY_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5698774.1"
                     /translation="MTYINVCLLYFCTVVCCLLLVSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            complement(21612..23669)
                     /locus_tag="ACHKAY_000023"
     CDS             complement(21612..23669)
                     /locus_tag="ACHKAY_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5698775.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTQDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHT
                     RPAGNETASSSPSWYYVIVIAGQSNASSYGEGLPLPDSYDRPDPRIMQLARRNTQTPG
                     GIPCKYNEIIPADHCLHDVQNMSLLNHPKADLKKGQYGCVGQGLHIAKKVLPVIPADA
                     GILLVPCARGGSAFTTGAVGSFDPASGAAEASLRWGVDTPLYQDLVSRTKAALEANPK
                     NVLLSVVWIQGEGDLKATPAQHGAQFTAMVKKFRADLGAFSGQCIGGSSDSVPWLCGD
                     TTYDWKEKNPAAYETIYSGYKNRASENIHFVPLMHDEFGNKTATNLPEHDPDIASAGY
                     YGAESRGDGNWTTASRATHFSSWARRFLVSDRFASAILKYAGLTSAFIAGVAPDGEAL
                     PQPAPGGKPEAPTGSQPGGSESPHPTSPSLRTLLSLKASAGEAPAQGWKITDGTIQLT
                     DEGKALTVSKQSGKTWTLTHGTDAAGELLKHGGQLTCKFRLSGEVKEGQFALGIYLHT
                     DTVPEGVTMSGTGTPFLMAYYLQTTGGQLNLMHHKTGGNTKLGSFGAYNNQWHTLVLK
                     FTAGSAQVTPVLDGTPGQAFQMVKDALSPAKDTLTLTDVTKGATYGVSFESVVLEVNT
                     PAA"
     gene            complement(23760..24320)
                     /locus_tag="ACHKAY_000024"
     CDS             complement(23760..24320)
                     /locus_tag="ACHKAY_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5698776.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(24334..24513)
                     /locus_tag="ACHKAY_000025"
     CDS             complement(24334..24513)
                     /locus_tag="ACHKAY_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698777.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISVLKGIFTSF"
     gene            24542..24745
                     /locus_tag="ACHKAY_000026"
     CDS             24542..24745
                     /locus_tag="ACHKAY_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5698778.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            24825..25346
                     /locus_tag="ACHKAY_000027"
     CDS             24825..25346
                     /locus_tag="ACHKAY_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5698779.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(25381..26292)
                     /locus_tag="ACHKAY_000028"
     CDS             complement(25381..26292)
                     /locus_tag="ACHKAY_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5698780.1"
                     /translation="MFHVDNNSGVANMPALAPVQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPGDVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     DQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPKNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(26292..26852)
                     /locus_tag="ACHKAY_000029"
     CDS             complement(26292..26852)
                     /locus_tag="ACHKAY_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO5698781.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            complement(26843..27925)
                     /locus_tag="ACHKAY_000030"
     CDS             complement(26843..27925)
                     /locus_tag="ACHKAY_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5698782.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(27925..28362)
                     /locus_tag="ACHKAY_000031"
     CDS             complement(27925..28362)
                     /locus_tag="ACHKAY_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO5698783.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(28355..28969)
                     /locus_tag="ACHKAY_000032"
     CDS             complement(28355..28969)
                     /locus_tag="ACHKAY_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5698784.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(28959..30083)
                     /locus_tag="ACHKAY_000033"
     CDS             complement(28959..30083)
                     /locus_tag="ACHKAY_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO5698785.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(30067..31416)
                     /locus_tag="ACHKAY_000034"
     CDS             complement(30067..31416)
                     /locus_tag="ACHKAY_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFO5698786.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIIDPLFIPGGHSVEVISEQQG"
     gene            complement(31403..33478)
                     /locus_tag="ACHKAY_000035"
     CDS             complement(31403..33478)
                     /locus_tag="ACHKAY_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5698787.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPPSPSSGGAGFWGRMMGRAGGLLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGAL
                     WAGAMAAPVLLDGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNY
                     LGGWLTEAWQKLRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNV
                     WNGGNYGLW"
     gene            complement(33605..34081)
                     /locus_tag="ACHKAY_000036"
     CDS             complement(33605..34081)
                     /locus_tag="ACHKAY_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO5698788.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            complement(34096..34461)
                     /locus_tag="ACHKAY_000037"
     CDS             complement(34096..34461)
                     /locus_tag="ACHKAY_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5698789.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            complement(34470..35972)
                     /locus_tag="ACHKAY_000038"
     CDS             complement(34470..35972)
                     /locus_tag="ACHKAY_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO5698790.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            complement(35969..36214)
                     /locus_tag="ACHKAY_000039"
     CDS             complement(35969..36214)
                     /locus_tag="ACHKAY_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO5698791.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            complement(36215..36775)
                     /locus_tag="ACHKAY_000040"
     CDS             complement(36215..36775)
                     /locus_tag="ACHKAY_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO5698792.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            complement(36772..37191)
                     /locus_tag="ACHKAY_000041"
     CDS             complement(36772..37191)
                     /locus_tag="ACHKAY_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO5698793.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASV
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            complement(37188..37574)
                     /locus_tag="ACHKAY_000042"
     CDS             complement(37188..37574)
                     /locus_tag="ACHKAY_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO5698794.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAEAGQDAPRKKTGNKAEQARA"
     gene            complement(37618..38565)
                     /locus_tag="ACHKAY_000043"
     CDS             complement(37618..38565)
                     /locus_tag="ACHKAY_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO5698795.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            complement(38565..39689)
                     /locus_tag="ACHKAY_000044"
     CDS             complement(38565..39689)
                     /locus_tag="ACHKAY_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO5698796.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            complement(39866..40339)
                     /locus_tag="ACHKAY_000045"
     CDS             complement(39866..40339)
                     /locus_tag="ACHKAY_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5698797.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            complement(40461..41792)
                     /locus_tag="ACHKAY_000046"
     CDS             complement(40461..41792)
                     /locus_tag="ACHKAY_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO5698798.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(41776..43365)
                     /locus_tag="ACHKAY_000047"
     CDS             complement(41776..43365)
                     /locus_tag="ACHKAY_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO5698799.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVSWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            complement(43365..45029)
                     /locus_tag="ACHKAY_000048"
     CDS             complement(43365..45029)
                     /locus_tag="ACHKAY_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698800.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            complement(45029..45610)
                     /locus_tag="ACHKAY_000049"
     CDS             complement(45029..45610)
                     /locus_tag="ACHKAY_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO5698801.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            complement(45613..45903)
                     /locus_tag="ACHKAY_000050"
     CDS             complement(45613..45903)
                     /locus_tag="ACHKAY_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO5698802.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRACVPGVKKPRARG"
     gene            complement(45900..46208)
                     /locus_tag="ACHKAY_000051"
     CDS             complement(45900..46208)
                     /locus_tag="ACHKAY_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO5698803.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            complement(46189..46416)
                     /locus_tag="ACHKAY_000052"
     CDS             complement(46189..46416)
                     /locus_tag="ACHKAY_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5698804.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRQAVDTPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARNVGQHR"
     gene            complement(46426..46644)
                     /locus_tag="ACHKAY_000053"
     CDS             complement(46426..46644)
                     /locus_tag="ACHKAY_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698805.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETRRQHEFTDMAGTPAVPAGKTK"
     gene            complement(46628..47056)
                     /locus_tag="ACHKAY_000054"
     CDS             complement(46628..47056)
                     /locus_tag="ACHKAY_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698806.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            complement(47091..47591)
                     /locus_tag="ACHKAY_000055"
     CDS             complement(47091..47591)
                     /locus_tag="ACHKAY_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5698807.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(47663..48088)
                     /locus_tag="ACHKAY_000056"
     CDS             complement(47663..48088)
                     /locus_tag="ACHKAY_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO5698808.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(48158..48667)
                     /locus_tag="ACHKAY_000057"
     CDS             complement(48158..48667)
                     /locus_tag="ACHKAY_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO5698809.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSVLLAVTGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            complement(48664..48960)
                     /locus_tag="ACHKAY_000058"
     CDS             complement(48664..48960)
                     /locus_tag="ACHKAY_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698810.1"
                     /translation="MDSSSQLFWYLYITSFFGAFVITRWLCRKVISIFHKKYLIGQAA
                     TSLINALARYHKGECYSRIKTDDGFNIFVIPPEYRVQLDRENNTYHIRRSVDTA"
     gene            complement(48950..49147)
                     /locus_tag="ACHKAY_000059"
     CDS             complement(48950..49147)
                     /locus_tag="ACHKAY_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698811.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            complement(49140..49472)
                     /locus_tag="ACHKAY_000060"
     CDS             complement(49140..49472)
                     /locus_tag="ACHKAY_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698812.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            complement(49488..49838)
                     /locus_tag="ACHKAY_000061"
     CDS             complement(49488..49838)
                     /locus_tag="ACHKAY_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698813.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTL
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            complement(49853..50164)
                     /locus_tag="ACHKAY_000062"
     CDS             complement(49853..50164)
                     /locus_tag="ACHKAY_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698814.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            complement(50161..50712)
                     /locus_tag="ACHKAY_000063"
     CDS             complement(50161..50712)
                     /locus_tag="ACHKAY_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO5698815.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRI
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            complement(50716..51231)
                     /locus_tag="ACHKAY_000064"
     CDS             complement(50716..51231)
                     /locus_tag="ACHKAY_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698816.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(51231..51764)
                     /locus_tag="ACHKAY_000065"
     CDS             complement(51231..51764)
                     /locus_tag="ACHKAY_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698817.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            complement(51768..52310)
                     /locus_tag="ACHKAY_000066"
     CDS             complement(51768..52310)
                     /locus_tag="ACHKAY_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698818.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            complement(52408..52938)
                     /locus_tag="ACHKAY_000067"
     CDS             complement(52408..52938)
                     /locus_tag="ACHKAY_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5698819.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            complement(52950..53243)
                     /locus_tag="ACHKAY_000068"
     CDS             complement(52950..53243)
                     /locus_tag="ACHKAY_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698820.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            complement(53248..53520)
                     /locus_tag="ACHKAY_000069"
     CDS             complement(53248..53520)
                     /locus_tag="ACHKAY_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698821.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            complement(53517..53798)
                     /locus_tag="ACHKAY_000070"
     CDS             complement(53517..53798)
                     /locus_tag="ACHKAY_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO5698822.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            complement(53800..54054)
                     /locus_tag="ACHKAY_000071"
     CDS             complement(53800..54054)
                     /locus_tag="ACHKAY_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698823.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            complement(54067..54288)
                     /locus_tag="ACHKAY_000072"
     CDS             complement(54067..54288)
                     /locus_tag="ACHKAY_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698824.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            complement(54291..55223)
                     /locus_tag="ACHKAY_000073"
     CDS             complement(54291..55223)
                     /locus_tag="ACHKAY_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5698825.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            complement(55295..57385)
                     /locus_tag="ACHKAY_000074"
     CDS             complement(55295..57385)
                     /locus_tag="ACHKAY_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO5698826.1"
                     /translation="MWVTAKECVGLPDLPMMEHNIRHRLTKAAAGNAAMLRKRKGTKA
                     IEFNIDCLPSGAQQVLRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQHPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            complement(57396..57662)
                     /locus_tag="ACHKAY_000075"
     CDS             complement(57396..57662)
                     /locus_tag="ACHKAY_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5698827.1"
                     /translation="MRVSRQASNNLFVREGCDWSAKAVYAALENKGTDLPELERELGL
                     KSGSMRNVFYRQCRKYEAAIAEKIGVEPAVIWPSRYQVDAREVA"
     gene            57814..58455
                     /locus_tag="ACHKAY_000076"
     CDS             57814..58455
                     /locus_tag="ACHKAY_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5698828.1"
                     /translation="MTNSYEEKTDVKREKLDRIISRSTINRFGERLKVAMKGMSNAEL
                     ARRSGMSETTIRKYLQGKIYPALDSLAIVADACGVSLTWLATGEQQANQTIVRDDAKQ
                     NYDNEYELLIHLLHKLKKEECVLLVDFISREGVNTLIRLVAMNASTISQDAVESIIDT
                     LPLRPVLKNAIKVGMAGSEDTDKEILRVIEKYNSSNNATQESRAVTVKKNVVG"
     gene            complement(58813..59106)
                     /locus_tag="ACHKAY_000077"
                     /pseudo
     CDS             complement(58813..59106)
                     /locus_tag="ACHKAY_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
     gene            complement(59432..61783)
                     /gene="ygjK"
                     /locus_tag="ACHKAY_000078"
                     /pseudo
     CDS             complement(59432..61783)
                     /gene="ygjK"
                     /locus_tag="ACHKAY_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(61800..62870)
                     /gene="ygjJ"
                     /locus_tag="ACHKAY_000079"
     CDS             complement(61800..62870)
                     /gene="ygjJ"
                     /locus_tag="ACHKAY_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO5698829.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(63004..64437)
                     /gene="ygjI"
                     /locus_tag="ACHKAY_000080"
     CDS             complement(63004..64437)
                     /gene="ygjI"
                     /locus_tag="ACHKAY_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5698830.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(64500..64949)
                     /gene="ebgC"
                     /locus_tag="ACHKAY_000081"
     CDS             complement(64500..64949)
                     /gene="ebgC"
                     /locus_tag="ACHKAY_000081"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO5698831.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(64946..68038)
                     /gene="ebgA"
                     /locus_tag="ACHKAY_000082"
     CDS             complement(64946..68038)
                     /gene="ebgA"
                     /locus_tag="ACHKAY_000082"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO5698832.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(68222..69205)
                     /gene="ebgR"
                     /locus_tag="ACHKAY_000083"
     CDS             complement(68222..69205)
                     /gene="ebgR"
                     /locus_tag="ACHKAY_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO5698833.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            69424..69756
                     /gene="ygjH"
                     /locus_tag="ACHKAY_000084"
     CDS             69424..69756
                     /gene="ygjH"
                     /locus_tag="ACHKAY_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO5698834.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(69798..71177)
                     /gene="ygjG"
                     /locus_tag="ACHKAY_000085"
     CDS             complement(69798..71177)
                     /gene="ygjG"
                     /locus_tag="ACHKAY_000085"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO5698835.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            71595..73115
                     /gene="aer"
                     /locus_tag="ACHKAY_000086"
     CDS             71595..73115
                     /gene="aer"
                     /locus_tag="ACHKAY_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO5698836.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(73269..73892)
                     /gene="nfeR"
                     /locus_tag="ACHKAY_000087"
                     /gene_synonym="yqjI"
     CDS             complement(73269..73892)
                     /gene="nfeR"
                     /locus_tag="ACHKAY_000087"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO5698837.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            74169..74933
                     /gene="nfeF"
                     /locus_tag="ACHKAY_000088"
                     /gene_synonym="yqjH"
     CDS             74169..74933
                     /gene="nfeF"
                     /locus_tag="ACHKAY_000088"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO5698838.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(74987..75062)
                     /locus_tag="ACHKAY_000089"
     tRNA            complement(74987..75062)
                     /locus_tag="ACHKAY_000089"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75027..75029),aa:Ile,seq:cat)
     gene            75187..75693
                     /gene="mug"
                     /locus_tag="ACHKAY_000090"
     CDS             75187..75693
                     /gene="mug"
                     /locus_tag="ACHKAY_000090"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO5698839.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(75772..77613)
                     /gene="rpoD"
                     /locus_tag="ACHKAY_000091"
     CDS             complement(75772..77613)
                     /gene="rpoD"
                     /locus_tag="ACHKAY_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO5698840.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(77808..79553)
                     /gene="dnaG"
                     /locus_tag="ACHKAY_000092"
     CDS             complement(77808..79553)
                     /gene="dnaG"
                     /locus_tag="ACHKAY_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5698841.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            complement(79664..79879)
                     /gene="rpsU"
                     /locus_tag="ACHKAY_000093"
     CDS             complement(79664..79879)
                     /gene="rpsU"
                     /locus_tag="ACHKAY_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO5698842.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            80117..81130
                     /gene="tsaD"
                     /locus_tag="ACHKAY_000094"
     CDS             80117..81130
                     /gene="tsaD"
                     /locus_tag="ACHKAY_000094"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO5698843.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(81173..82636)
                     /gene="ttdT"
                     /locus_tag="ACHKAY_000095"
     CDS             complement(81173..82636)
                     /gene="ttdT"
                     /locus_tag="ACHKAY_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO5698844.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(82684..83289)
                     /gene="ttdB"
                     /locus_tag="ACHKAY_000096"
     CDS             complement(82684..83289)
                     /gene="ttdB"
                     /locus_tag="ACHKAY_000096"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO5698845.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(83286..84197)
                     /gene="ttdA"
                     /locus_tag="ACHKAY_000097"
     CDS             complement(83286..84197)
                     /gene="ttdA"
                     /locus_tag="ACHKAY_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO5698846.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            84404..85336
                     /gene="ttdR"
                     /locus_tag="ACHKAY_000098"
                     /gene_synonym="dan"
     CDS             84404..85336
                     /gene="ttdR"
                     /locus_tag="ACHKAY_000098"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO5698847.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(85349..85966)
                     /gene="plsY"
                     /locus_tag="ACHKAY_000099"
     CDS             complement(85349..85966)
                     /gene="plsY"
                     /locus_tag="ACHKAY_000099"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO5698848.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            86071..86439
                     /gene="folB"
                     /locus_tag="ACHKAY_000100"
     CDS             86071..86439
                     /gene="folB"
                     /locus_tag="ACHKAY_000100"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO5698849.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            86529..87350
                     /gene="bacA"
                     /locus_tag="ACHKAY_000101"
     CDS             86529..87350
                     /gene="bacA"
                     /locus_tag="ACHKAY_000101"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5698850.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(87513..88751)
                     /gene="cca"
                     /locus_tag="ACHKAY_000102"
     CDS             complement(87513..88751)
                     /gene="cca"
                     /locus_tag="ACHKAY_000102"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO5698851.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(88815..89435)
                     /gene="ygiM"
                     /locus_tag="ACHKAY_000103"
     CDS             complement(88815..89435)
                     /gene="ygiM"
                     /locus_tag="ACHKAY_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO5698852.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            89677..90978
                     /gene="ygiF"
                     /locus_tag="ACHKAY_000104"
     CDS             89677..90978
                     /gene="ygiF"
                     /locus_tag="ACHKAY_000104"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO5698853.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            91001..93841
                     /gene="glnE"
                     /locus_tag="ACHKAY_000105"
     CDS             91001..93841
                     /gene="glnE"
                     /locus_tag="ACHKAY_000105"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO5698854.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            93889..95322
                     /gene="hldE"
                     /locus_tag="ACHKAY_000106"
     CDS             93889..95322
                     /gene="hldE"
                     /locus_tag="ACHKAY_000106"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO5698855.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNITSLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(95442..95501)
                     /gene="ibsE"
                     /locus_tag="ACHKAY_000107"
     CDS             complement(95442..95501)
                     /gene="ibsE"
                     /locus_tag="ACHKAY_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO5698856.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(95818..95877)
                     /locus_tag="ACHKAY_000108"
     CDS             complement(95818..95877)
                     /locus_tag="ACHKAY_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5698857.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(96116..97777)
                     /gene="yqiK"
                     /locus_tag="ACHKAY_000109"
                     /pseudo
     CDS             complement(96116..97777)
                     /gene="yqiK"
                     /locus_tag="ACHKAY_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            complement(97804..98433)
                     /gene="yqiJ"
                     /locus_tag="ACHKAY_000110"
     CDS             complement(97804..98433)
                     /gene="yqiJ"
                     /locus_tag="ACHKAY_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5698858.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            98702..98902
                     /gene="glgS"
                     /locus_tag="ACHKAY_000111"
     CDS             98702..98902
                     /gene="glgS"
                     /locus_tag="ACHKAY_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO5698859.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(98945..100010)
                     /locus_tag="ACHKAY_000112"
                     /pseudo
     CDS             complement(98945..100010)
                     /locus_tag="ACHKAY_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(100012..100761)
                     /gene="yqiH"
                     /locus_tag="ACHKAY_000113"
     CDS             complement(100012..100761)
                     /gene="yqiH"
                     /locus_tag="ACHKAY_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5698860.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            complement(100777..103281)
                     /locus_tag="ACHKAY_000114"
     CDS             complement(100777..103281)
                     /locus_tag="ACHKAY_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5698861.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            complement(103341..103892)
                     /gene="ygiL"
                     /locus_tag="ACHKAY_000115"
     CDS             complement(103341..103892)
                     /gene="ygiL"
                     /locus_tag="ACHKAY_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5698862.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT        24662 a        27374 c        26561 g        25331 t
ORIGIN      
        1 tctaatacac caactttttg gaaacaatat tgtaagatca aatttatatg gtggcgaaaa
       61 ttaatctctt atcacggctt acgccgcttt tattcgtgtt cgctcctttt ttggcacagt
      121 caaatatgac tgtttatcct atggctgttt ccattaatag tcagggtgaa ggcaacgttc
      181 gcgtaatatc taaaagtaat gaagttcagt acatcaaggc gacagtattc cgtatcgata
      241 atccatcgac gcctcaggaa aatgaagttg agattaaatc cggtgatgca aatcatctgg
      301 ttgttatgcc acctaaattc gctttaccag ccggtagtag caaaaccgta cgttttgttg
      361 cgatggaacc agagcaaaaa gagaaaaatt atcgcgttaa atttgaagcg gttcccagta
      421 ttgatgacgt tgccacagat aaaaaagatc tctctatgca gttaacagtt aacttaattt
      481 gggggattgt tgttagtgtt ccacctcagc aacctattgc taagttagaa gtaaatgctg
      541 cccaaaaatt agttaatgca gggaatcaac gcttaaagat tttaacgatc gcttattgta
      601 aaaataatag caaagaaaat tgtaagatac agaccgtaaa taaaaacatc ttccctggtc
      661 aggaaagaaa tcttgaaagc atatctggct acgacaaaat cgttgtcaaa tataataact
      721 ggatcaccaa agataatggc gagtttgaac tggcggtcca ttaattatca ttgtaataaa
      781 cgaaggaata gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag cgatgttttc
      841 tgttgcaggc tccgcattgg cagtacaaaa ggatattact gtaacggcca acgttgatgc
      901 cgctctggat atgacacaga ccgataacac cgcgttgccg aaagcagtag aaatgcaata
      961 tctgccgggg cagggtctgc aatcttacca gttgatgacc aaaatctggt ctaacgacgt
     1021 taccaaagat gtaaaaatgc agttagtttc cccggcgcaa ctggttcaaa gcctggatgc
     1081 tagcaagatt gttccgctga cagtaacctg gggcggtgaa gaaattaaag ctgatgcggc
     1141 aaccactttt accgcaacca aaatctttgc ttccgatgcg ttaactaacg gttctctggc
     1201 taaaaacctg atgtttgctc aaatcaccaa aggtgttctg gagacaggca tctaccgtgg
     1261 tgtagtaagc atttatctgt ctcaggatat ctaatccaga taacagaatt aacacgtaac
     1321 aaagggaggg gtagccctcc cttattttaa ctgttttagt atttgttatg gatttcagga
     1381 ttgccatgga taaaaaatta ctggctcttt tgatcctggc gagtctcagc ccggcagagg
     1441 cgacattaac caaaattccc gcagggtttg aggttattgc tcagggacag caggagtata
     1501 tcgaggttta tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt aatcttgata
     1561 ccgtaacatt tcttgatcca gcaagtctat ataacaagct ggaactggat gtagacgatc
     1621 agaaaatcgc gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc cacggtgaat
     1681 tggcttgcgg ttatgtacgt actgactcag ggtgtggttt tctgaatacc gatacgctgg
     1741 aaataatcta taatgatgaa gaaagttcgg caacgttgtt tattaatccg caatggaatt
     1801 cagctttcga tgcgaagtca ttatatttaa atccagacaa aaatactgtt aatgctttta
     1861 tacatcagca agacatcaat gttctggcac aggatgatta ccaatcgttg tctattcagg
     1921 gaaacggtgc gctgggaata acagaaaata gttatattgg tgcacactgg aatttcaacg
     1981 gttatgatgc agatgatgtc agtgacagta atgctgacat cagcgatctc tattatcgtt
     2041 atgatttttt acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc acactattta
     2101 atgcacaagg cgggaacttt acctttaact ttctgccact cggtgcaatt gatgggatgc
     2161 gtatcgggtc gacactgagc tatttaaacc aggcgcaaag ccagcaagga accccagtaa
     2221 tggttctgct ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa cttttgggat
     2281 cgttttatct caatagtggt tcgcaattta ttgataccag ttcctttccg ccaggcagct
     2341 acagcgtggc attaaaagtc tacgaaaata accaactcac ccgcaccgag cttgtgccgt
     2401 ttaccaaaac cggcggtctg actgacggaa atgcgcaatg gttcttacag gcaggtaaaa
     2461 ctacatcaca ggtttctgat gatgaaagtt cagcttatca gctaggagta cgcctgccat
     2521 tacatccgca atatgagctc tacgcagggc tggcgaatgc cgatgatgtg agtgctttcg
     2581 agttaggtaa taactggacg gcagatttag gcggggtggg gaatcttgca atcagcgcca
     2641 gcgtgttccg taacgatgac ggcggcaaag gtgatatgca acaggccaac tggagtaatc
     2701 cgggatggcc gacgttgggc ttttatcgga ccaactctga cggcgatgct tgtacaaccg
     2761 acagcagaga gagctataac gccttaagct gttatgaaag tatttccgcg acggtttcac
     2821 agaattttgt cggctggaat atgatgctgg gttatacccg cacacaaaat aacactgatg
     2881 atagtttgcg ttgggataaa cagcagagct ttgaaaataa ctatcttcgc cagacaacag
     2941 cgcaaagtat ctctgaaact gtacaactta gcgcttcccg cgcttttgtg atgcgtgact
     3001 ggattttgag tacgtcagtc ggtgttttcc atcgtaatga caacggtggc gataacgacg
     3061 acaacggctt gtacttatcg ttttcgttat ctgacacgcc aacgatggat agcaataaca
     3121 acagccattc aacaaatgtg tctacggatt atcgttatag cgaacaggat ggcgatcaaa
     3181 cgtcatggca gttatcgcat accttttata acgattcatt cagccataaa gaacttggcg
     3241 taaccgtcgg aggcctgaac accgatacca taaacagcgc ggttaacggg cgttgggatg
     3301 ggcaatacgg aaatgtctac gctaccgtat ctgatagtta tgaccgtaag aatcatgatc
     3361 atctttcggc ctttacgggc acttatagct ctacgctggc tgtcagtcgc tatggcgtta
     3421 atttgggagc cagtggtaca gacggtttgc tgggtgcggt actggtggat gtgaaaggct
     3481 tctctgaaca ggatgaagag agtcaggatc tgcaactcga agcgcgggtg gcaggcagcc
     3541 gaactttgca gcttggtcaa agtgacagtg tgttgttccc ttatcctgga tttcagtctg
     3601 gttttgttga ggttaacgac agtagccagg gcaatcagca ggggacaaca aacatcatta
     3661 acggtgcagg gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat cgcgaagtgt
     3721 ctgccagctt taactacaac tatatcggtc gtttattact gccggcagcg gtgaaaaaat
     3781 tcccgattgt ggggctgaac agcgccatgt tactggtagc tgaagatggc ggatttacac
     3841 ttgaaattaa cggcagcgaa aaagagctct atctgctttc cgggcagcaa ttccttaagt
     3901 gtccactgag tgttgtaaag aaacgcgcca gcattcgtta cagcggagat gttacttgta
     3961 gtgtggtgac ttattcacaa ttaccggagt cgattcaggt tcaggcacag ttgaaacagc
     4021 ctaaattacg tggaaacgtt cagacggcgc aaagggaggt tgcaccatga gaaatcgatt
     4081 gattgcggcg atattgggct tgtttggttc aggcagctcc tgacgtgacc agtgaaatta
     4141 cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag
     4201 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
     4261 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
     4321 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgccag
     4381 gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga tcttcatgta
     4441 cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc taccttgagc
     4501 agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt aaattacatt
     4561 cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc gaccccaacc
     4621 atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag ttagacttgc
     4681 atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg ttggacatgt
     4741 gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc aaagatgaag
     4801 ggcgaccagc tgcagggcgc agagacggtt acttctctat ctatcgtcag ggagggacca
     4861 ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg gaaaccggtg
     4921 ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac ctgaaacgtg
     4981 tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg acacccttga
     5041 ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac atgggcaaat
     5101 tgtcagtaat ctttacgcct tccttgccaa ccatcaatta accctgcaaa ccggacaaac
     5161 gcttttttct atataaacga cgaagccgtc tgataaagcg gcttcgtcat tacaacttaa
     5221 gttaaacgtt aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc
     5281 ggtttgttct gcaacacggt ccagataacc agcgcaccta acaggtcgaa cactgccaga
     5341 actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgacaac cagcgcaaac
     5401 agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta
     5461 cgaccgaaca cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg gtgggcaaaa
     5521 ccaccgatac acagcagcat aattgcgaca tacgggttgg tgaacagacc gatcataccc
     5581 gggccaatca tcagcacagc acccagcgtt acgaccatct tacgggaaac gatcaggttc
     5641 acaccaaacc aacgctggaa cagcggcggc aggtaaccac cgaggataca accgaggtca
     5701 gcaaacagca tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact
     5761 ttaaacatga acagcgggat ccacgcgtta aaagtacccc aggccggttc tgccaggaaa
     5821 cgcggcagcg cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc
     5881 ttcgccgtgc tcacctggtg ctgggcttcc tgaccattaa taatatagtc gcgttcttca
     5941 tcggtcagat gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag
     6001 ataaagctca atgcaccgga gatgataaat gccatctgcc agctgtgcat tacgattgcc
     6061 cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtaacct
     6121 accgcgatgg aacgctcttt cgccgggaac cattcggagc tggctttcag acccgccggg
     6181 atcatcgctg cttccgcggc accgactgca ccacgagcaa tcgccaggcc accccagcta
     6241 ccagccagcg cggttgcacc acagaacacg gcccacagca cagcaaacat tgcataaccg
     6301 attttcgtac ccagcacatc cagtacataa cctgctaccg gttgcatgac cgtataagca
     6361 gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc
     6421 agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc
     6481 gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc
     6541 gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt
     6601 acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc
     6661 taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt
     6721 gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac aactttaaaa
     6781 ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acagcgaggc
     6841 cagtactggc gcggcctgca gagagattta ccactttgag agtaattttt ttaactacgt
     6901 ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc
     6961 agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt
     7021 tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc
     7081 aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga
     7141 ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc gatcactaca aatggcgcgc
     7201 tatgcgtacc aacggtgtgg ccgagcgtct gtgtaccggt gatgcgtctg accgtgaaaa
     7261 atttgacgcc tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac
     7321 gcacctcgaa ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc cgtcaactgc
     7381 cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct ccgcgcgcgg
     7441 catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct
     7501 ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg
     7561 gcgtccggac aaagccttca acatcgaaca ggcgaccttt aacgactaca tggcgaagct
     7621 gggcgaagtt tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa
     7681 acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt
     7741 gatgtttgct gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggctgg
     7801 cgaaaccctg agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg
     7861 tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa
     7921 caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg
     7981 tccgatggca gaagagttgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc
     8041 gaaaaccatt ctgtactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg
     8101 taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa
     8161 cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtttgctgag
     8221 ccgctttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata
     8281 cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc
     8341 ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta
     8401 cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg
     8461 ctggataacg tcgcggtcgc tttagcggat ttggctgaag gcacagaagt cagtgtcgat
     8521 aaccagactg ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat
     8581 atcgcaaaag gggccaatgt catcaaatat ggcctgccga ttggttatgc attggcggat
     8641 attgcggcgg gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat
     8701 cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcgtgaagtg
     8761 cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg gatcctgcca
     8821 accgtgggct gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc
     8881 aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc acacctacgg ctgctcacaa
     8941 ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac
     9001 gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt
     9061 gaaacgttgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat
     9121 gatgagatcg aagccgggat cgagcatttg catcagctgt ataacgtgat gcgcaacgat
     9181 aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac
     9241 ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct
     9301 aacggcggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg
     9361 atggaccatt gccgcgacga agcaacgttt gaaaaactgg tgaccatggt caacgacttc
     9421 aaacagtact ttattgccca tgaccagccg atctatgaaa acccatcgcc ggggaacaaa
     9481 gcgggcggta tcaccacgct ggaagataaa tcccttggct gtacccagaa agcgggttcc
     9541 agcgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccggg gctgaacttg
     9601 ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat
     9661 atggtgctgt tcagtactgg tcgtggcacg ccatacggcg gctttgtacc gacggtgaaa
     9721 atcgctacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt
     9781 cagctgatcc acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt
     9841 gagtttgcca acggtaaaca aacctgcaac gagcgtaacg acttccgcga actggcgatc
     9901 ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca
     9961 taatgtgaag cgccgtttgc ttttccatac ctgataagga tttatcatga ccgcgtattg
    10021 gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg
    10081 tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt
    10141 ttttcttctg ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt
    10201 gattacctta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg
    10261 aattggcctc gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt
    10321 cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc
    10381 aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca caatgattga
    10441 gggtagtttt ctgcttatga atggcttgaa tatcattcgt ttctggcgga tgcagaaaag
    10501 gggaattgat ccgtttaaag tagagaaaac ccctcccgcc gtagacgaaa ggggttaaac
    10561 aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca
    10621 gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg
    10681 atgataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta
    10741 caggccagcg ggatcagcag cagagaaccc cccgccacgc cggatgcgcc acaggcacac
    10801 agagaagcca ccacgctcaa cagcagcgcc gtgggcagat cgaccgggat acccagcgta
    10861 ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg
    10921 gcacccagcg gaatagaaac ggaataggta tcgcgatcca gattcagctt ttcacacagt
    10981 gccatattca ccggaatgtt cgccgcagag ctgcgggtga agaaggcata cacgccgctt
    11041 tcgcgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc
    11101 aatgggttaa ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc
    11161 gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagaccaaaa
    11221 ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa aggtgacggc attcgacata
    11281 tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaaacc gaggccaatt
    11341 gcccacacca ggatcccgat gtagttacct ttcagcagcg cgtcgatggg gttggaaacc
    11401 atgctcatta ccagcccgcg catcacttct acaatgcctg acggtggcga aatatcaccc
    11461 gcgctactgg ataagtgcag ggtagaaggg aaggcgaagc tgaaaacgac tgcggccaga
    11521 gcagcagaga aggtacccag cagatagagg aacaaaatag ggcggatatt ggttttctgc
    11581 ccgtgctggt ggttagcaat agatgccatc accagcatca acaccaggat gggggcaacg
    11641 gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt
    11701 gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg
    11761 ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt
    11821 cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa
    11881 gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt
    11941 ttaaagagag gtcatatttt gcaacttaat ggttacattt atttgacata aaaataaagc
    12001 caacgtgcgg cttccagact gatgacaaca tcaaaaaagg actcggtcag tcccctcgcc
    12061 ccgtttgggg agagggttag ggtgagggga acaggccagc atcggtgcga aaaattaccc
    12121 tcatcccgac cctctccctg gaagggagag ggagaaaacg gtgctcatgt taatgacagg
    12181 gtacacggct taggcagatt aaaaattatc caccccgctg cttatcatgc cgataattca
    12241 cccaggcgtt gataataaac gtcatcacca gaatgccaaa caccacgccc agcgagacgg
    12301 cgattggaat atggtagaag tcgacaatca gcatcttgat accgataaac accagaatca
    12361 ccgccaggcc atatttgagc atcgagaaac gctctgctac gcccgccagc aggaaataca
    12421 tcgcacgcag gccgaggatt gcaaacaggt ttgaggtcag cacaataaac ggatcagtgg
    12481 tcacggcgaa gatagccgga atgctatcca cggcaaaaat cacgtcgctc aattccacca
    12541 gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc attcttacgc acaaagaaat
    12601 gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc accagcggct
    12661 tgtcgccaat gcctgattca tcttcatggg cgagcgccat cttcacgccg gtaaacagca
    12721 ggaaggcacc gaagatatac aggatccagt cgaactgtga aatcagccag ctgcctgtga
    12781 agatcatgat ggtacgcaga acaatcgccc cgagcacgcc atacaccagc acgcggcgtt
    12841 gtaatgccgc cggaacagag aaatagctga acaacatcag ccagacaaag acgttatcga
    12901 ccgccagcga tttctcaatc agatagcccg tgagaaaggc cagtgcctgt ggatcggcaa
    12961 cggcgcgacc ttcagtttgc accagatacc accagaaagc ggcgttaaac agtaacgaca
    13021 gcgtcaccca gaccagcgac caggccgcag cctgtttcat ggtcatggca tgtgccccac
    13081 gacgcccctg caacaacagg tcgatagcca gcataatggc gacaacaaca gcgaatccgc
    13141 cccatagcaa cggcgtgccg acagtattca tagaagttcc ttacacataa aaaaacggcc
    13201 aacgtcggaa gacgtcagcc gctgcttttt atgcatagac ctcgccttcc ggcaaggtct
    13261 cacttacaac aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg gtgaccggat
    13321 gtggtttttc acacatcgta atgacgatcg accggcaatg aagttactcc cctttgcagg
    13381 taacaaagta tgacagacca ttcagccagt caatggtctg ttacatacct tttacactgt
    13441 tttactttgg caattatagt tttacgctat ctgccggaaa aatcacccca gtctggcggc
    13501 ggatctcggt cagcagtttg gcggtgatgc gactgaccgc cagccccgga tgatccacca
    13561 gatgctcatc caccagggta gcgaacagct ctgcttcgta gagcatggta ttaatatgct
    13621 gcggctgggt gagatcctgc atttggctgc cacgcggcac gaagcacact ttctggcatt
    13681 cagacagttt ttcaatcacc agcgatcctg cttcgccctg aatctcgctc gccaggacag
    13741 aatcactgac ttttgagtgc tgcaaggtga cgctgaaatc accgtaatcc atcaccacca
    13801 cgccgtgggc atctacaccg cttgccagca aactggcggt tgcctgcacg cttttcggct
    13861 caccaaataa cgccaccgcc gacgccaggc agtaaaagcc gatatccata attgaaccgt
    13921 tagagaatga cggattaaag gtgttgggat tttcaccgtc caggtaacgt tgatagcgcg
    13981 aggaatactg gcaatagttg aaaaagactt tacgcaattt gccgactttc ggcagcgcct
    14041 ggcgcagcaa atgaaagttc ggcaggcagg cggttttaaa tgcctcaaac agcaccacct
    14101 gattttcccg cgcacaggca atggcggcat ccacttccgc cagattcgac gccagtggtt
    14161 tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg
    14221 cattcgggct ggcaatatac accgcgtcaa tggcatcgct ttccgccatc gcttccagcg
    14281 aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg ctgagcctgc tcaaggctgc
    14341 gggaatatac ggcggttaac ttgtatttac cgctctcatg ggcggcctcg acgaactggc
    14401 gagtgatcca gttcgtacca atcacagcga aacgtatcat aacgcgtgga tactccataa
    14461 agggattctg tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca gatacgcatt
    14521 attcttttgc cggacagcca tgctcactaa acggcccaat catgaccgat ggcccgagat
    14581 atttcggctg gcgatggagg attttggcgt caatcacggc gctgacacga gcgagtttct
    14641 cccgcagttg agtataaaaa ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag
    14701 cgtcgatgcc gtactgtttt gccagccaga tggcgcgttg attatgaaat tcttgcgaga
    14761 taatggtgat atggttttca ccaaaaacct ttttggctcg cactaccgaa tccagcgttg
    14821 agaatccggc atagtcgcag aagatcactt tcgccggtac gcctttagcg atcaagcctg
    14881 catacctgag gcttcatcgt aatttttacg tccgttatcg ccacttacca gtagccattt
    14941 cacttttccg gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt
    15001 acccggcctg gcccccagca ataagcccac gttacgcgcc ggaactgcgt tgacatcgct
    15061 ccaggtcagc tgcttgctgg cgttgaccat aacgcgatcg gcaataaaaa tggtcgctcc
    15121 tgctataagc aacaacaaac agccaatctt aagagtccgg cgagaaaaac aaatacggag
    15181 aagaaggcgg ggctgcatgg tcgttatcct taacgaaagg ggggaggtcg attttaggcg
    15241 ctaaagaaag aaaagataac gcctgacatc attttcagat tttgtcctgc ccaagcgaca
    15301 actgcgttag ccacagccgg gtgtcgaact caagctggtg gtactgcggc tccatatggc
    15361 agcacaactg gtaaaacgct ttgttatgct ctttctcctt caggtgcgcc agttcgtgca
    15421 ccacgatcat acgtaaaaac ggttccggcg cgttgcgaaa cacagtagcg acgcggatct
    15481 ctgcttttgc tttcagcttg ccgccctgca cacgagaaac agcggtatgt aacccgagcg
    15541 cgttttttag cacatggatc ttattgtcat acatcacttt attgatcggc ggggcattgc
    15601 gcagaaactg attttttaga tccggagtat attgccagag ggctttatcg gtggcgtagt
    15661 cgtgtgttcc cggatagcgt tttgccagca catcgcccag acgctgttcg ttaatcagcg
    15721 ttcgcacctg ggaaagtagc tgctcgggat aaccctgaag ataagtaaga ttgctcataa
    15781 ccgcccacga aaaagagaaa aagggtatac tcacgcaccc ttttcagggg caacgtcgaa
    15841 attttatcat tcaggagggc cgatgagcca cttagacaac ggtttccgtt cactgacact
    15901 acaacgtttt ccggcgacgg atgacgttaa cccgctacag gcgtgggaag cggcggatga
    15961 atatttgctg caacagttgg acgacacaga aatccgcggc ccggtgttga tcctgaatga
    16021 tgcctttggt gcgttaagct gcgcgctggc ggaacataag ccgtacagca ttagcgactc
    16081 atacatcagt gaactggcga cgcgcgagaa tttacgcctc aacgggattg atgagtcgag
    16141 cgtgaagttt ctcgacagca ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa
    16201 agtgccgaaa acactggcat tgctggaaca acaactgcgt gcgctgcgca aagtggtcac
    16261 gtcggataca cgtattattg ccggtgccaa agcccgtgac attcacactt ccacgctgga
    16321 actgttcgaa aaagtgctcg gcccgaccac caccacgctg gcatggaaaa aagcgcgcct
    16381 gattaattgc acgttcaatg aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa
    16441 gctggaaggt actgactgga ctatccataa ccatgcgaat gtcttctccc gcaccgggct
    16501 tgatatcggc gcgcgcttct ttatgcaaca tctgccagag aatctcgaag gggagattgt
    16561 cgatctcggt tgcggtaatg gcgttattgg tctgacgctg cttgataaaa acccgcaggc
    16621 gaaagtggtg tttgtcgatg aatcgccgat ggcggttgct tccagccgtt tgaacgttga
    16681 aaccaacatg ccagaggcgt tggatcgcag cgagtttatg atcaacaacg cgctctccgg
    16741 cgtggagcct ttccgcttta atgctgtgct ctgcaacccg ccgtttcacc aacaacatgc
    16801 gctgaccgat aacgtcgcct gggagatgtt ccaccatgcc cgccgctgcc tgaaaatcaa
    16861 cggcgagctg tatattgttg ccaaccgtca cctggattac ttccataaac tgaagaagat
    16921 tttcggcaac tgcaccacca tcgccacgaa taataaattt gtggtgctga aagcagtgaa
    16981 gctggggcgt cgtcggtaag attgggagtt acctgccgga tgcggcgtta acgccttatc
    17041 cggcctacgt tcagcacgtt actcccaggc cggataagac gcggcaagcg tcgcatccgg
    17101 caatgtctgc ccagttctgg aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc
    17161 tctacattct cttgtttagc gttgttcaac gtttatgcta catacgtgaa gttatgacgc
    17221 ataaagaata cgatttcttt accgctgttc atcgtactaa ggggaaaaaa tgattgcgat
    17281 tgccgacatc ttgcaagcag gagaaaagct aactgctgtg gcaccttttc tggcgggtat
    17341 tcagaacgag gaacaataca cccaggcgct ggaactggta gatcatctgc tgctcaacga
    17401 tcctgaaaac cccttgctgg atctggtgtg tgcaaaaata accgcgtggg aagaatcagc
    17461 gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta tagccgtgat
    17521 tcgtaccctt atggatcaat atggtttaac cctttccgat ctgccggaaa ttggcagtaa
    17581 atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac acgctaaaaa
    17641 attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacgcg acgtgccgga
    17701 tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc agcgccagcc gtgttccctg
    17761 ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg cagccgccaa ttaaatgcac
    17821 agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg tttggctctt gccctgcgca
    17881 gatcaccaca ttgtccactg ctaatacctg cgtttcgccg ttgatcacca catgcagccc
    17941 gtcatcgtca atcttctgat aacttacgcc tgggatcatt ttcacacccc gcgagagcag
    18001 ggtggtgcga tggatccagc cggtggtttt gcctaacccc tgtcccggtt tgctggcttt
    18061 gcgctggagc atcacaattt gccgtgggct acgcgggatc tgcattccct gcgggcttaa
    18121 gccaccagcc tgttgtaggc tgctgtcgat cccccactca ttacagaacc tggcgatatt
    18181 ctggctggtg gattcgcccg gctgacttaa atacatcgcc gtatcaaaac caatcccgcc
    18241 acaaccgatg atggcaacct tgttgcctac cggcgctttg tcgcgcagta catcgagata
    18301 actcaatacc ttcggatgat cgatcccgtc gataggcgga acgcgcggca cgatcccact
    18361 ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc gtcacggtgt gattgagttt
    18421 cagcgtcacg cccgtcactt cgatcatccg gtggtaatag cgcagcgttt cgtaaaactc
    18481 ctctttgccg gggatctgtt tggcgatatt aaactgcccg ccgatctcgc tgtgggcatc
    18541 aaacaatgtt acatgatgcc cacgcgccgc cgcgttaatg gcaaacgcca gcccagcagg
    18601 tcccgcaccg accaccgcca gatttttttt ctgcacggca ggaaggattg gcattttggt
    18661 ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact ttgccaacga agatctgatc
    18721 gaggcaggcc tgattgcagc caatgcaggt gttgatctca tcggctcgcc ccgattgcgc
    18781 ttttgacagc agttccgcat cagcaagaaa cggtcgtgcc atcgatacca tatcggcatc
    18841 gccgcgcgag agaatgtcgt cggcaacctg cggatcgtta atccggttgg tggtcaccag
    18901 cggcagcgaa acgtggcctt tcagtttgcg cgtgacccaa gtaaatgcgc cgcgcggcac
    18961 gggcgtggca atggtcggaa tacgtgcttc atgccagcca atgccggtgt tgataatggt
    19021 cgcgcccgcc gcttcaatgg cctgcgccag ctctaccgtt tcggcaaaag tcccgccgtc
    19081 ttcgaccagg tcgagcatcg acagtcggta gataataatg aagtcgttgc cgacgcgttc
    19141 gcgcaccgca cgcactactt ctacggcaaa tcgcatccgg ttgcggtaat cgccgcccca
    19201 ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg ttgatcaaat acccttcgga
    19261 ccccattacc tctacaccgt cgtatcctgc ctcccgtgcc agttgcgcgc agtgggcgaa
    19321 atcgtcgatc agttgcagga tctcttcatg ggtgagttcg tgaggaacga aacggttgat
    19381 gggggcctgc aatgcggagg aagcgaccag atgcggttgg tagctgtagc gcccggtatg
    19441 caaaatttgc agggctatta tagcgcggcg atttttttga taccgatttg catttcattt
    19501 gataccgctg aaaaacgccg atacgcaact tatttgatac taaatcggcg cttttctttt
    19561 gagttttaga atgagttttt aagcgctcat tcactctctt tcaaaccagt tttaactgtt
    19621 tgtggttttg ggtaagggtg gactttgaat agctttgtgt tgaggcgttt tctggccctc
    19681 ttgttcagaa accggatgta acgaaactgt cggaacttat gcacactggc tctgtcgata
    19741 ttagccctca gatgttcacc tcgttgtccg cctcgtttaa tggcactcct gcaaatctcg
    19801 tgataccact cgccatcaag ttcatagaat gttgattcat ggctgcctac ataatcaaaa
    19861 ttgctggcct gatacacgac accaagacag ccacaacgct catctgcaaa cgactgaacc
    19921 cactgtacct gcggataaag ctgcctgatt agcttgagcg cgtaactgat tgcccttgat
    19981 tcggagtttc tcggcataca gtcatgcatc catagccggt taagctccat atattctcgg
    20041 ttctgcgtac ccgttacgac gcgtgcacca ctgtttgggt taagggcgta accccattgc
    20101 attacgccaa caagttcccg ttctgaaaat atacccagat gaaggtagga attatttacg
    20161 aaacggtggc tgtaatgttt attgacgata accagtctgg ccagccagca acttattgtc
    20221 tcaacccgca actcacggga gccataacca acgatattgt cgttatagcg aataagttca
    20281 ggcgtgctga tgatacggga tgtgacttgc tttctgttcc ccacgacagg atttccttgt
    20341 gtatgtgggg tgctctgtgg cgctcggaga tgtgatttga ttgagggttt tacagcgcgg
    20401 acattttact tccagataac tgaaactggc ccgtgccagt agcttattgc agtgtcggca
    20461 ccgtatgttt cgatacatgg ccgcataacc tcctgctttg ttgttgatgc ctttctcatg
    20521 caatcgatcg acaaaaacga tcgattcgat ttgtttaatt gatacaacaa atagatttaa
    20581 aagtcatcaa caatgattca gggcggtgat tatttactta aagaaacgaa gagttaggtg
    20641 gtgaaaacga tcataaaatg atcattttat tgacttttta tacagtgaag gtaggatcgc
    20701 gcgtatatgt agtatgtttc aaatcaacag gatactacat aaaagtgaaa cccagcatag
    20761 ctggaactat gctgggtcaa tctgtaaaaa cttgcatcaa tgctttactt cgagctcgtg
    20821 gcgtatccta tgatattccc cggtctcctg taaagcagag atgcataaaa aggagatctt
    20881 tatcatggat aaagagcctg tttgccctgc ctgtaagact cccttacagc tcatctatcg
    20941 ccgttctcga tctcaaaatg gcaagacata caaaccaaaa aatgcttcct gtttcaggat
    21001 gctgatatgt cctgcctgtt ctccccggag ggaaaataca caaaacgtgc attaacaaat
    21061 tcacccggca gggaaacctg ccggtcggta ttgttacatc atagtaccgt cagttccggg
    21121 ctgaaccggc tctggttgct ctacgggttg aggctcattt tccgtgctgt tttcagccgg
    21181 agaacgacca accattcggg ccagcacttc atcaacatat gcgtcgattt gtgcctcaaa
    21241 atccttgcga acctgcgcct tcagcacgcg gttcacttct tcagaataaa gtgcctgttt
    21301 gacaagctct tcagtaaccg tgcctttaat ctctggcatg aatattctcc tttccttttc
    21361 tggttcacaa aaaattaaat ctgaccggag tctttcggct gcggcattac agcaccgctt
    21421 tttatctttt cctcggcagc tgcatcaatt ttatttcgaa aataattgcg aataatttta
    21481 taaccaccgc tgacaagcaa cagacaacaa accaccgtgc agaaatacag caggcagacg
    21541 tttatatatg tcatataagc ctcactctta ttaactgatg ttacagccgg ataaattccc
    21601 ggccgtcacg attatgccgc aggggtatta acctcaagga caacgctctc aaaagacacg
    21661 ccataagttg cccctttggt gacatctgtc agtgtcagcg tatctttggc aggggaaagt
    21721 gcatctttca ccatctggaa tgcctggccc ggagtgccat ccagcacagg cgttacctgg
    21781 gcacttcccg ccgtaaactt caggacaagt gtatgccact ggttattgta ggctccaaat
    21841 gatcccagtt tcgtattccc ccctgtttta tggtgcatca gatttaactg gccgcctgtt
    21901 gtctggagat aatacgccat caggaacggt gtgcctgtac cactcatggt tacaccttcc
    21961 gggaccgtat cggtgtggag gtaaataccc agagcaaact ggccttcttt tacttcacct
    22021 gacagacgga atttacaggt caactgacca ccatgcttca gaagttcacc ggctgcatca
    22081 gttccatggg taagcgtcca ggtttttccg ctttgtttgg atacggtcaa cgctttacct
    22141 tcgtccgtca gctggattgt accatctgta attttccagc cctgagccgg ggcttctcct
    22201 gctgatgctt taagtgaaag cagcgtacgg agtgacgggg atgtcggatg gggggactca
    22261 ctgcctcctg gctgactacc tgtcggcgct tccggtttcc cacccggcgc gggctgaggc
    22321 agggcttcac catcaggggc cacaccggca ataaatgcag aggtcagacc tgcatattta
    22381 agaattgctg atgcaaaacg atctgagacc agaaaacggc gcgcccagga gctaaaatgg
    22441 gttgccctgg atgctgttgt ccagttgcca tctccccgtg attccgcacc ataatacccg
    22501 gcagaagcga tatccgggtc gtgctccggc aggttggtcg cggttttatt accgaactca
    22561 tcgtgcatca gtggaacaaa gtgaatgttc tcggaagccc ggtttttata tccgctataa
    22621 atggtttcat atgccgccgg gttcttctct ttccagtcat aggttgtgtc accacataac
    22681 cacggcacac tgtctgatga accaccaata cactgaccgg agaaagcacc aagatccgca
    22741 cggaattttt tcaccatcgc agtaaactgg gcaccatgct gggccggggt cgctttaagg
    22801 tctccttccc cctgaatcca tacgacagat aacagcacat tctttggatt cgcctcaagt
    22861 gccgctttag ttcgactgac aagatcctga tacagtgggg tatccactcc ccagcgtagt
    22921 gacgcctcgg ctgcaccact tgccggatcg aatgagccaa ctgcgccggt tgtaaaagca
    22981 gagccgccac gggcacaggg aaccagtaaa atcccggcat cagcaggaat caccggtaat
    23041 acttttttcg caatatgaag tccctgcccc acacaaccat actgcccttt tttcaggtct
    23101 gcttttggat ggttcagcag actcatattc tgaacatcat gaaggcagtg atctgctgga
    23161 atgatttcat tgtatttgca gggaatacct ccgggggttt gtgtattcct gcgtgccagc
    23221 tgcataatac gtggatcagg acgatcataa gaatccggaa ggggcaaccc ttcaccataa
    23281 gatgaagcat tggactggcc ggctataaca atcacgtaat accaggaagg ggagctggaa
    23341 gccgtttcat ttcctgccgg acgggtatgt gtttctgcat tatcccagtc tgacggttca
    23401 gcagtaccgg caacatcagc acttaacgga atgacagcac caatacccgc tccggtaatt
    23461 tccacgttcg gatcaccgga tactggcctt acccacaggc aatcctgtgt attaaaactg
    23521 atgagacagt cagcaaatgt cattcctcct ttcgtttcag caggaggaaa gggagcctca
    23581 agaaatgccg ctgtaccacc ggaaacttta acggtaaagt ttccgggcat tgatgatagt
    23641 tgaacccatt tggacgtcat aatattcatc ctgttcctga ataaacagaa ttatcatatc
    23701 cacagaccga taaacgccat tttgcgttaa ttcagtaaag gcccaacaaa gggagcgtct
    23761 tatttttccg aaacgctcgc aggataataa cgataaaggg ttgaaaggcc gatatcaaaa
    23821 ataagtgcca cctgtcttcg actgacaccc ccggccagta aacgcccgac ctgctcgtat
    23881 tgttcctgcg tgagacgacg gggtctgccg ccggttcgtc ccctttcacg ggcagctgca
    23941 agaccagccc gtgtccgttc gacaattaat tcacgctcca tttctgccag cgcacccatt
    24001 atgtgaaaaa agaagcgccc catgggggtt gacgtatcaa tgttgtcagt aagactacga
    24061 aaatttatcc cctgttgctg taattcatct gtcagcgcaa caaggtggcg catactgcgc
    24121 cccagcctgt ccagtttcca gaccatcagt gtgtcaccgg gttttagttt cttcagtgcc
    24181 cgcttcagtc ccggccgctg tgtcgtttta ccactcattt tatcttcaaa aatctgctca
    24241 catcctgcac attccagcgc atttctttgt aaacctgtgt tctggtcatt tgttgatacg
    24301 cgtatatagc caatcagcat gtatttacct tccttaaaaa gaggtgaaaa tgccttttaa
    24361 aacagatata cggcatccct gaaacctcgg tttacgggat actgtaaaca gggccgcgaa
    24421 tgccctgcca tcgaacggag cggctgttgc agcgaataaa ctcgccactc caagaaatat
    24481 taatggtgtc ccatttgacg gaacgcagga tataaatatt acatcgggga tgacgcagtc
    24541 tgtggctgac tcacgttatg ttcaaagcat tcgaagaggt tcaaggtcaa cgattggaat
    24601 gcaatataat atttttgagg ttcctgacgg atgcgtactt acagggcttg atgtcgctgg
    24661 tgatggtaat gcaacggtaa ccgcatatta tcgacctgtt cagtttctga tagatggttc
    24721 gtggaaaaca gcttccagcg cataaaagtg cagtagttca aaaaacagat tatctgaata
    24781 aatatatgat gattttgtct tttcaacaaa aacaaggtga aataatggag cttattaacg
    24841 taaaacgata ttacccggaa cacaaaccct atggtgagga tgttcagtat ttccagagtg
    24901 aggacggaag ggatttttat gaatctatcc cgcttttcac aaagaaatac aaactctgta
    24961 tttcgcctgt aacgggtatt atctgctctg ttgctgaaga tgtctctgct ttatatccgg
    25021 caggttttac tgttgttgaa gtggacgaac tgccggaagg tgtgaatatt gacggtaact
    25081 ggcagttttc tgatggcctg atatcaaagg tgccggtaaa ctggaagacg gttgctgaaa
    25141 agcgtcgctc atcgctgctc caggaagcca acgaaaccgt ggatgactgg aaaacagaat
    25201 taaaactgga catgattagc gatgaaaata aattacagct gacgcgctgg atggcgtata
    25261 tccgacagct taaagaaatg cactttaatg atattgccag tgaggggcat taccaggcga
    25321 ttccctggcc ggaaaaaccg gaataattca gagggtgcat tcagcaccct tttctgtggt
    25381 ttacactgaa cttaccgttc tccaggcatt attaatataa atctgaaccg ggcgataata
    25441 gaccccaccg atattgtccg cagaccggga gccggtttcc tgcacgttaa taccggaaag
    25501 catacaaccg gaaggtgcgc tgtaattcca tgatatttca ttgcctccgg gtgaatgata
    25561 gctctgtgct ccaagctgaa cattctgtac ataccggcca tctgcggttg actgcgtcat
    25621 tcctgacgtg atgttgatat cctgcgttcc gtcaaaagga acgccattaa tattttttgg
    25681 ggtggcgagt ctgtttgctg ccacggcggt tccacccgat ggcagggcat cagcagcctt
    25741 atttaccgtt tcccgtaaac cgacgttatc gataaaagcg cctttatccg gaatgtctgc
    25801 gccattctgg tccctgcgca ggtaacggtt atcaccttcg ctcttgctgt aaacatccag
    25861 attattacgg gcggttccct tattctccag gtcggcgaga ttctgatcct tcgccagggc
    25921 attaacgtca ccgggaccaa gactgttttt tgtggcaagc gtccccagac caagataacc
    25981 acgggccaga cgctgggcct ccgcaccggc atcagcaatt tctttcaggt tatttttccg
    26041 ggaaaaagca ttcgcagcga taatggcttt aatagacagc gtaagctggt ttaactgcgc
    26101 cttatccggc tgaatactgg cttcagccag aatattcagc agttcggcct ggagaatatt
    26161 cagccagtcc tgaccaatcc agctgatacc tttttgtccg tcaccttcgg taaaccaggt
    26221 ggtggtatta ctctgcaccg gtgccagcgc aggcatattc gccacgccgg aattattatc
    26281 aacatggaac attaaatatt ctcctcttcg tgactgtttg catatacgta aataaacgtc
    26341 tgccatgcag gcttataacg gttcagaata cattccagcg caccgccttc ataaatacgc
    26401 agcggcgtga gaatatcatc cagcacattc atattccggt agccggtttc actgttaatc
    26461 gtgacaatac tgacccactg tgaatccggt gatggctgaa tatctatttc ataaccaaac
    26521 tctgccgcaa accggatata aaattcacgg ttaagagagg gtttcatccg gtatttattc
    26581 ccggcataac gctgacgctc ctgaatggtt gcgccggtca tatcacattc cggtaacccc
    26641 agataacgct cccagtcttc cagcagcaga cgggatgaat caggaaaacg ttctgccagc
    26701 atctggttac cggtccagga aacacgctcc gtggaatgac tgaggcccag gcacaaagcc
    26761 gcaagaacgg atgacggccg cttatcccat gccagcccgt caggcagcaa ctgcaacagg
    26821 gcgcgctgat gcggggtcag ggttacagcc atgtgatttc ccccacggtc agcaactctg
    26881 tatctccggc catcacggac ttcagcgggc tgcgaacttc aaaatcctcc agtcctttca
    26941 cccctgcaac agcccgccag aatgatgaag gcaatacaag tccacccggc cgcgattcgt
    27001 tataaagcag gtctgttaat gcctgtttta cggcagcctg attttccggc gttttgggta
    27061 caatcctgat ctcaaacgcc accggcttat tggtcagctt aaacacgctg attgtcggcc
    27121 caagtggctg accaacgggc tggcccgtcg ccggatcatc atgactgcgg atataatccg
    27181 ccacccgctt cacatcacct tctccgggga aaatgtcagg gttattatcc tgaacaaaag
    27241 tcacaccaac actacccgcc tcaggccatt caggcttaca ccatgcccgc gtcacgcccg
    27301 gtacttcccg tgcccagcgt tcaaaatcat actgtgttcc ccctgatggc gggttctgta
    27361 cccggaatac cagcctggac agcaactccg gcaccgtttc cacatccgca ccaccggtga
    27421 gtcccgtgcc ggtgaccgtg gctgtctggt tgatgcccgc ctgtggcgtg ataaaggaca
    27481 ggcgggttcc ggtcggggta tttccagcgc gccccgcact ttccgcctcc acattaacat
    27541 tcagtgttcc ggcttttccg gtcgcagagc cggtgatgcg gtacacaacc ccatcgcttc
    27601 gctgaaggag tacgccttcc gtgatccctg catccgtggt cagcatcagt tgtaccggtc
    27661 cgtcagcgcg ggaagccggt ttacggatga caccccagaa tgcgcagtgt ttcaggagtt
    27721 cggcttcatc agcttctgtc ggaattatct gccgggagat ccaggccaga tgttcatgct
    27781 cctgtgctga cagacccgcc agtgcatacg caatggcatt cagggtggtt tcattcacgc
    27841 ccggctgcga accgggcagg cgctggctga tatcctgttg tgtctgtgtg atcagttgcg
    27901 ccagtggtaa gggctgataa ggcatcattc ccccttcata tcagcataaa aaatcatcgg
    27961 atgtctgctt ccgtccggca gggtgatttt cacggaaagc gaaaggcgag catgccccac
    28021 acgcatggca tgacatacca cggatgtggc agcaccgctt ttatgcagcc acgccagcgc
    28081 ttcatcagca taggcctgtg cacggctgac cacggaggac agcgtctttt cacggcttaa
    28141 cagccagaga cgggagccaa cggggcgctc ccggaaactg tctccccacc atccccgacg
    28201 gtcacgggtg ccgtcaggga tttcatcgga atccagcgcc cgcctgtccg taaacagtga
    28261 aataatgacc gcagtcagca ggctgttatc cgtcagaaga tctgcgccgt tcagttgcag
    28321 ggaaccgcaa ccctgatccc agacgacagc aatatcagcc attctctggt cctcccgttt
    28381 tgctgccttc accgttatcg tgatgcacat gacctgaata ggtgatcccg gcaataaccg
    28441 cttcggacat cgtgaacgtc ccctgtgatc ttccggtgcc gtcaatggac acattgcctt
    28501 tgacatgcag gttcttatcc actgtgacat taccggtaaa gtgagcctcc ggtgtatcca
    28561 cctgaacacc ttcatcggcg taaatctcca gcgtcttaca ggtcacaatg atccggccgt
    28621 tctttgtcag acgtatgcgg tgcccctcat ggtgatacac gccggtatct ccggcggtaa
    28681 gtccggtcgg gcgactgcgg cggtcttcca ccacaagcac cacggcctga tcccgttgtc
    28741 cgccagcaca ggccagaaaa acttccgcac caggcagggg gacgctgatt tgcccgtact
    28801 gctgggggcg ttcaacgtca tcaaaggttt caccatccat accggtgatt tgcacattct
    28861 ggattttcag cgtgtccgtt gtgccggtca gtacaccacg cccgaacagc agacgaacac
    28921 cccgcatcac gggggccaga aggcggctga tgacttcgtc attcatcatt gaatttaatc
    28981 ccctgttttt tcatctgtgc ccgcacgaag gcgtccacgt cacccgcatt tccgcttccg
    29041 ctgtttcccg gctctgccgg aacaataaag ccgtcacggg gtgccagaat cagacgggtg
    29101 gtttcgccgt gatttgcatc cagcgaaaac tccacctgac agacaagaag atcacgctct
    29161 tccactccgg tacggggagc cgtgacagcc gtcagcaggt tgatatccca caactggccg
    29221 ttctcccgaa accagtgccg cacggtggct gtcaggcgca cagagcgggc aaccgcccgg
    29281 cgcatttccc ggacagccct ctggcgtgca ccgtctgcat caacagcatg atccgccagc
    29341 acaattttcg gtctgtaacg gtggatggca ctgtctgtca ccgtaccaac tggtgcggcc
    29401 agcgttccgg ctgtcagtgc atccccggca tgaccaccac cacgcccgtg cccccgtaca
    29461 cggtattcac tgtggcgcag acggtgatcc acgttgtgat ccaaatccag cagattttcg
    29521 cccagcgtca gcgtgtcacc acgctgactg ccggcctgag tgaaaaccag ttcaccggcg
    29581 gcattactgg ttaccaggac gcctctgtgt ctcgcggccc gtgtcagcgc atctgccacg
    29641 gtttctgaat tttccagtgt gaaggtggaa aagggccggg ctgccgttgc atcatttacc
    29701 tgccagcgca ccgtgacccc gaacggagca cacaaatctg aagcaatgtg ctccagcgtg
    29761 cggttcctcc actggctgcc cggatgaatg gctgaacagt ccaccaggtc accggtttta
    29821 tcccgtccgc tgagcgtgat ctgaaaacgc gtggcgctga tgcgctgccg gacctgatcc
    29881 agccagccag tgatcacagg ctgaccgtca atgcgcaatt ccagagactg accggcacgg
    29941 acggatgacg gaaggcgtac accgggcatc attaccccca gttcaaagga gcccgccaga
    30001 tgttcaagag agcgacgaac actcaccgtc agccagccgg aaaaaatctc accgcccaga
    30061 tacagttcaa ccctgctgct cactaatcac ctccactgaa tggcctccgg gaatgaacag
    30121 cggatcaata ataccgttac ggcgcacaaa acgttgccag ccggtactgt tcccggtggc
    30181 acggtacagg gtcaccagtg caggctcggt ggtacgtaca gtcactactg ttgcaccggg
    30241 tagctgaact ccccgtgtat tcagatcctc tgtcagtgcc agtctggcat cacgaagcgt
    30301 cagcgccgtg gcggtatacc cctgcccgga cagtgtcatc acctgccgtt cgagcgcttc
    30361 gcttaagtca cggttaatgc gctgaagatc aactgaactt tccagccaga caggccaggt
    30421 acggtgggct gcgtcactgt ccggcagggt ttcctgactg agaacctgat ccagcagttt
    30481 tcccacggtc tgtgcctggg ccaccacggc agcactctgc attaccgccc gtatcagacg
    30541 aatattcttt tgtgctgccg gtgtcagacc gatgagagtc tgcggattat cctgctcatc
    30601 cagcacgtaa cgcagggaag ataatgtcct gtacagttgt ggcattccct gagagacggc
    30661 ggatgtcgtc cggccgatac tgccaccggg ccgggtattc aggcctcctg aagacagtga
    30721 cggcagcgat ggcagggtga tcagggcact gaaaagcccc gcaaaagatg aggccatgcg
    30781 ggaaggtgct gtcagtaatg ccgtggcatt ccctttcagc gccgtgaacg tggcagtaaa
    30841 ggcactgata tcctgtacaa tccccatgcc ggaaacggca ttttccaggg cggacacctt
    30901 atcgctgatg gtttcggtca ttgcctggac atcgtgcatt ccgtcagaaa tcaccgtcca
    30961 gcccgcttcc agggtttcaa aaaccttccc gagcgcggtg gtactcttct gctccagaat
    31021 ggcggccgtg tcctgtgtga cagcaggtgc ggtatcatca cttaccggcg tgacattaat
    31081 ggtgaactca accacgccct gttcagcagc gttataacga ctttcaaagc tgtttatcag
    31141 cacatttaac gtgccgtaat ccgggtgaag cagctccccg gcaccgggag cacgaagggc
    31201 atcacgcaga cgatttcgct gtgtctgaac atcatccccc atcacaagta cactgaacgt
    31261 aaattcagcc agtttaggtc ccagatcgtc tgcaccaccg gtttcccgta acggatattc
    31321 ccgacggaca atgtttcgcc cgccacgttc gcgctgctca cgccagacga ggaacggcac
    31381 accacggaac gcaccacgac cgtcaccaga ggccataatt ccctccgttc cagacattca
    31441 catccagtcc gtgaccggac gtgtcatcaa tatcaatact gcgagcctgc cacccttccg
    31501 gggcaaccag ctccacacgg gcagaggctt tttgtaccgc ctgtccactg ctttcatcat
    31561 cactgccccg taatttctgc caggcttcag tcagccatcc ccccagatag ttaccgagat
    31621 agctgcccac cgtagaacca atggcaatcc ccacaggtcc ggctgccgcc cccagcgcac
    31681 caccggcaat actgcctgca agagagccga ccgcttctcc cttgtctgtg gcactggcgc
    31741 tgccatccag cagaaccggg gctgccatcg cgccagccca cagggcacct ccacccagcc
    31801 ggccggccag tccaccggca ctgcggaaaa agccggagaa acgccccagc cccatgcggc
    31861 taccgacaga cgaaagtaac ccaccggcgc gccccatcat gcgcccccag aaccctgccc
    31921 caccagaaga gggagacggt ggcactgggg acggtggcag gggaggcgtg accggtcttc
    31981 cgcgccccgg tccgcgtttc ctgcggcccc gcccctgacg tccgcctgag gatgtatcgg
    32041 tcgcgccggt gccggacagt cctcctgtgg gccagtttgt tacaagtact ggctgaacgg
    32101 ctgccggatt taccccggta agaaagttta taaaacgttg ccctcgtccc tgagatgtgc
    32161 ctggtggtgt tccggccgga ggagagcgcc ggaaaggtga ggtcaggaag gccgccgctg
    32221 ataatccgta tcccagcaat gcagcacccg gacgtataat ccccgttccg acagcccgcg
    32281 ccattttcag ggcacgggtg gcgagataca tgtacagcag gtatttcgcg gcggtctgtg
    32341 ccccctgacc aatgcgatcc agcgcatcac catagcccgc atcccgcaga gcctgaagcg
    32401 tgtcgcgtac ctttctgatc gcctgataaa accctgtcac tgcatcacgg gcatactcaa
    32461 agccctggtt catggctgat gccgtgcttt ccgccaggga atggtaactg ccatcagatt
    32521 tggcactgtc aacccatgaa agaaatcccc tgagattacc cttcagtttt tcaaatggcc
    32581 cactgtccat cacatcccgg gcaaactggt cccagacgtc acccatcatg gccgtcaggc
    32641 cactccagga attcattgcg tccttttgtg ctcccctggc ctgttccgcc agcgtctgaa
    32701 ataacaggcg aatactgtcc ggcccaagca gccctttttc accaagtttt ctgaccactt
    32761 tctggtccac acccagacga tcagccagca cgcggtaggc attaatgccg taggtggcaa
    32821 gaatatttgc atccgccgcc tgaatactgc ctcttgcata catctgtttc agttgcaggg
    32881 atgccccctg tgcatcggac agtgaccagc cgcccacggc accctgatcc tgaagcatgg
    32941 tgacaaaatt tcgggcctcc ctgtcactca taccaaagcc cagactggac gtatattcct
    33001 gcatgacgcc agccagcccc caggtggttt ccttcgcatt ctgtattgcc cacctgcgga
    33061 catcatcagt tttcgcccga tcccctttgt aaagggagtt caggcgaatc atataggttt
    33121 ccatctccgc tgccgggcga ataaatcccc ggttaaagcc atatacagca gcaccaccgg
    33181 ccagcattcc gtaaagattg ctgatccgcc cgatggtgcc ggtaatactg ccctgaagac
    33241 ggtcaaaatc agaagtgaca tggcggatac tgccccgcac cccggccagc gtgcgctgca
    33301 tcctgcttcc gagtacatct gtttcctgcc cggcacggcg ggcagcattt cccaggccac
    33361 ccagccccgc ctggccggag cgtgagaatg cccccagctc ctgtgaccac tggcgggatt
    33421 tggctgagat attgccgagc aaatcaacta tcagagatgc tctgagattt cgggccatac
    33481 ttactgcttc gttctgatga ttttttctgt ctgtcggcag tgccggtaaa gttgcgacag
    33541 ggggaggtta agcgcccatt ccggaccgct ttttgtcacc atccccagga taattgccgc
    33601 ttcttcaatc tgatcccgac actgcgcctg atcgcccccg gtcagctgcc agctttccgg
    33661 caagcgcggt atccagcagg ctgaccgctg ccatcaggcg ggagagatcg cgctcgctta
    33721 actgtccaat ctgacggggt gacaacggac cttcaatgtc tcccactgcg gcaatctgac
    33781 ggcgcagcag tgcaacaccg cgtaacgacg gagacgctat cagcatcggg ccattgtttg
    33841 tctccaccac gcgctcggct tctgcttctg cgtcaatact gtctttcgct gtcagttccc
    33901 ggaacgtgac acgatattca cgggtctcac caaacggcag accatcaagc agatccacat
    33961 atccctttgc cagttgctct gtcagcccgg cagtgcgtgg atcagccccc tcaagtgctt
    34021 cccggattgc ctccatcacg gctgcctcgg aaactgccgt ttttttctta cgtgtggtca
    34081 ttttattttt cctgatcact gaacacgggt actggaggcg ctggcgaact tcacggagat
    34141 ctccccgcca ccgtcaagcg aggccggttc actgctccag gccttcgtca tcatgtggac
    34201 ttcgccggta tccgccacaa actcgatagt gaccgcagtc cagttgttga tttcatcggc
    34261 ggctggtgaa ccttctccgc ctgccggaaa tttgcagtcc agcgtcgctt cacgtggttt
    34321 ctggcggtaa ccatagactt tcgcgccttt caccacttca cgttcaaaac cagacggaga
    34381 aaatgtagcc ccctcaagcg tctcgtattc ctgaccgttc acacggatag tggccgtacc
    34441 ctgatactgt tttccgctca tgccttacct cacaaaatga aacggatctg tgctgccagg
    34501 aagcggaact ggttaaccag atccggcgtg cataacacgt caagacggtt tcgatctgac
    34561 gcattacgct ccacaagcag attcttttta aaggtgtcca gattttcgac cagcccgagt
    34621 tccacccact cttcacccag cgaaattaac tgaagcttca tgatctccgg cgtcacgata
    34681 ttctgaccgg cacgaacggg cgtcccgtca tcagccagtt tatggcgcgg aaaacgttgt
    34741 gtgataaaag tacgcaggga ataacgcagc caggagaggg tgtagatggt ttccacatcc
    34801 agatagctgg gatcactttc gccgtacacg ttctggcgat acatggtcac ctgacgttcg
    34861 atctgaacca catcaccggc agccacactg accgtggcaa tccctcccca cagcagactg
    34921 ttacgctctt cacgcgtcag acgatcggcc ggagccgggg ccatgcgtga cggaagcgcc
    34981 agcgtctgaa gcggacgcgc aggatctgct cccagagagg gtgcacaggt ggcacagacc
    35041 gccgctgccc agaggtaatc cggttcaggg gctttgggaa tggatgtgca ggtcgccaga
    35101 aaatcattgc gttttgcccc gaatgcctgc acctcaccaa acgtgccggt atgtgccagc
    35161 cacagcacgc cgtcagacat tttggccggt ccccagcgtt tcagaagctc atcactgatg
    35221 attttcaggt ttgcatcatc cttatagggc atgacgacat agttgtactg gcgatcgccc
    35281 atacccgcca cgcttcgggt gatatccggg ttagccgctt tttgtttcgg ataggctata
    35341 gcgacctgta ttccttccgg ggttgcttcc ccgtcgtgat agttcaggcg cacatcatga
    35401 acggagcatt caccggtaaa gcgggcggtg atccccagag accctttcag acctgcgcca
    35461 ttatcaccac ttcctgcacc agaggaggcc gtgaagggcg cgtcaggatc ggcattgatc
    35521 agcgcggcca gctcgtcggt cagttctttg cctttcttac cttttggtgc cgggagtgta
    35581 taacgccgcc cccccacgca ggcataaaca atcccgtcac gggtgaccgt tccggtcaga
    35641 ttcatggccg cagccgttgc ctgtccggtg ccgttcccct gagcaatggc atacagttca
    35701 gcatcaggat tgatggcgat aaattccgcc accatcaggg caatcatgga gccgcgcccc
    35761 cagagttctg ttgcctgaga cgcacgggtg atcctgaccg gaacatccag aacaccggca
    35821 ccgtccacgg cattctcttt cattctcgcc tgaccaaaca gcagcacaca ctgacgctgt
    35881 gccggagtgc cggtgacagc catggagtta ttaaactcaa cctcaatcag cggtatacgg
    35941 cggtcattac cgatcccgtc aaaattaatc attgcggttt ctccttatcg gatgccggtt
    36001 gggcgaccgg tttttctgtc gcagtcgttg catccgcaga tacagccgga gcctcttcag
    36061 cggctttttc agcctgaatg acatcgccgt catcaagacg ccgacaccag aacggcgtaa
    36121 agggcttttc ctccccctcc tgtgccagcg ggcgcattgt gtcgggatca cgaatcaggc
    36181 gtcccggcgc gggtttgata aagatggttt tcatttacag ttcctccggc ggttcgaccg
    36241 ccgttgtcgt gttccccggc aaatcaatat gtgcctcaaa cggcggcgta ccgggcggct
    36301 ccacaaaggt ttcgtaatga cgcaggaagt cgtccagcga gctggtatcg gtcagcggtg
    36361 cgatcatttc ctcgcaggag aaataaagcg catacatcac cgcaccactg tccgcctggg
    36421 tttcggtgta accgttgacc gccttttcaa agtaaagcgg tgaggttttt tctgttctga
    36481 agccgttaag cacggcaatc agccgggcca caatctgata cagtccggga cggctggcct
    36541 cacgcccgtt gagcatgtcg ccgatgacgt agaacaccca gtgactgacc aggcgaccac
    36601 gggtacggcc ttcaccggca cccagccagg caacgtagat cgccggggcg ttaatcagca
    36661 tggtgcgcag tacgctgtcg ctccagtcgc cgggatgcgt gtcaacagac accagttcat
    36721 tcccgaaata ctcacggata cgggcgatgt atgcctgttc ggtttccgta atcatatgaa
    36781 gcccttctgg ttgcgcccga ataccgctgc atcagactga acctgtggta aatccccgga
    36841 ttcaggggcc gcaccgttcg tatccacacc gaccggcaca ttgccgttca tgacatcttt
    36901 cagccaggcc agcgcttcac ggtaacggtc acgcgcctga tcggaggctc gctgatcgca
    36961 caggtaataa aaggcaatcg cgcaacagtg ctgaacaaga acggccggaa taacctccag
    37021 cggcaacgtg taacgggcgg acagatagct gtcgataagg actgacgcat ctgtcagcgc
    37081 ccggttcagc ttgcgcgtgt ccggctcatc cgaacggggc acagccagta acggcctgag
    37141 caaatcctcg cggtaacgcg cccgcatatc ggtttcagtg gcgtaattca tgcacgggcc
    37201 tgttccgctt tgttaccggt ctttttccgg ggagcatcct gtccggcttc ggcttcggct
    37261 tcggcttcgg cttcggcttc ggcttcggct tcggctaaat ctgtaccggc cactgcatga
    37321 accacacccg ccacgctccc cgcatcatca gcagacgtct gtagtattcg cacagtgagg
    37381 catggctcct cctccagtcg ggcaagctgc tccggggtga cctcaacctg ctgacgtcca
    37441 cgcgtgaaca ggaaccccgc acgacgaaac gccgaacgac tgcaacgcac ttctgccagc
    37501 accgtgacag aattgtcacc actgacctga agacctgaat catctgttgc tgccacagtg
    37561 tgatgttcat tcatatttgc cctccgcagg ggccgggccg taagcccggc tcaccgttta
    37621 cagataatcc gcgacaacca gctccagctt acccttcatc tcgttagaga cggttgcgtt
    37681 tccgtctgcg aacagttcac gctccagcag ctgcaccgcc tgtttttcca gcgaggtggg
    37741 gacaacaata tgggtgggtt taatgccgag tttgcggcca ccgtcagcgg taaattcgcg
    37801 catggctgac cagccgtgcc acagtgcatc cagcgtcagc ggtgactgca tcatgtaggc
    37861 catctgccag aagccgtagc ccacattgcg acgggcggat gcaccaaaca caaattcgtt
    37921 atcggtgaat gcgcgacctt catcgacttt ggtctgggca accagttcag ccttacggcg
    37981 atcctgataa atcagcggtt ttaccgcgcg ggagcagtca agcagatacc aggccggacc
    38041 gctgtaatca gcctgtgcgc cgacagagcc ggtttttgcc acaaacatat tgctgaccat
    38101 ctgcgcatcc ccggagccat ccactttggg gtaaaccgga tgttcggtat caaagaagtt
    38161 ctgaccgtca tagcaggccg cactgatccc gtcacgcagg gcagcaaaga ccagctcatc
    38221 cggttgtacc gccgcagcgc gccccatctc ctggaacaac ggggaataaa taccgaggtt
    38281 gtcgtcttcg aagtcatcgc ggctaatggc gacagtgcct tcaaaagttt tgttcacgat
    38341 ggcatagcca taggctttca tcttctcgat gacgcgggag ccgatccact cacggaactg
    38401 cgggaactgc cccagccagc cgtaggtgtt ggatttcgat gtggagggca cggtcatcgc
    38461 aattttttta tactgcgacg gggccatgga catcccggcc tgaaaatcag aacggtagcc
    38521 cgtcatcagg gctgtgatca tcgccggtgt aatcggggta ggcattattg catttcctct
    38581 ttcattttca ggaactcggc ttcggtttta cccagcagtt tggcggccgc gatatcttca
    38641 gccgacagcg cagcggtggc agtcttttta tccggcacgg tcacggtgtc ggtctgaaga
    38701 gaggtcaggg ccgcaaccgg ctggcgggcg tcaagctgtg cagaaagtgc cgcaacgcca
    38761 atctgcccgc ccagttgttc cagatagccg cgttcgcttt tgaagatgcg cccttcggat
    38821 tcagccttgt ccagcacctg ctccagcgtg gtgctgccgt gctgcgccga cagcgcaacg
    38881 tattcggtac gcagggcgtt atacgtttca acgggcacgt atttcgtcag atcaataatg
    38941 ccgccagccg gtgtgccttt tgcggtttcc agctctgccg acagactggc gacctgtgtt
    39001 ttcaggttgt cgtgtgcatc cgcacgggtt ttgatcccgg tgagggcaga cagcgccgcc
    39061 gtgcccagct ccggcgtaaa ttcgtcaccg tcagccacgg tcagaccgag cgccgtcagc
    39121 aactggcgta attgctcatt catggaggta tcctttaaag gagggtttaa ggcgttataa
    39181 aggtcatccg ccgacagcgc ggcgacagga ttcatgccgg tcagaccggg gtcaccggta
    39241 atagccagca tccgaagttc gacaggttcg ccggtgttct cgtcatagcc aatcaccggt
    39301 gaaaaccagg ggaattcgtt attacgcaga tgttcaaggg ccggagggtt ccacttcggg
    39361 cgcacctcga agccacgctc ttcactgaag cgaaaattct ccggtgaggc catcacaaaa
    39421 ccggcagccg gtgcctgatg ccccttaatc agggtctggt ggttgtagtc aattttcacc
    39481 ggctgattga gcgccacaac ccgggatacc atgcgctcaa cggtcgcacg gttaatcagc
    39541 cagccctcgg ccggtttttc cgggcgaccg tcacgggcct ttacccgacc ggctggcatg
    39601 atctgacacc agtcaccgtc cccgtctgcg gacaggctga tggcattcag aatggcataa
    39661 gcaagtttgg gcgtgttttt cgtcttcatt ccggcagcat aagccgggaa ttttcagggc
    39721 gggttttgcg ggatttcagg aagtgcttcc aggggaaaaa tacaaaggcg cttcacgcag
    39781 attttaaagc ccgttaaata caggttagaa aatcacgaca cgcgcctgaa gggggtaagg
    39841 taatgcattt gcatacctta aacaattaag gcgtttctgt ggccttttta aagcgttttc
    39901 tgatttcgtc catgatctcc tgctctgcca cctgatcaaa gcccatatac gggcgtgcgc
    39961 caatggccgc cggtcccggt ggcataccag gcaggccacc ccactgatga atggccgcat
    40021 aaggctcatt tgatccaatc agcgcccagg tatcgccata atccgtggtc aggcgtcggg
    40081 ccagatcgcc gttcagcgtc aggattttgc cgggcatgta tcccttacgg gtacgccact
    40141 tgcggtaagg atctgaccag tcatgccagc gttcgccgtc cggctctttt tcctgttcaa
    40201 acgccatttc tgacgatgac agaagactcg ccgccacact gcgggccagg tctttccctc
    40261 cacccacaaa ctgaagccgg gcaaagaccc gctggagacg cgtaacgtca acaacgacag
    40321 cggcatcaat ggatgacata ttgcctccgc ataaaggatg aatataaaat aaacaggcgg
    40381 tcagtgtacg cttaactggt aaagtcggtg cctgcctccg ggtggatcat gtatgcgggt
    40441 tcaacccccg ccactgaccg ttaatcaata tctccttcca gtacctcaag cattccaccc
    40501 cggatatccg atttcagctt atccatattg ataacccggt aagcattcac aatcacatcc
    40561 agtttatccg gctgacgctt caggctgtac ggcgcattaa cggcaatctg cacactgcta
    40621 tccttacttt ccacgatata catcagattg ttgtggcgtt tgtcccacag caccgctttt
    40681 ggctttgcca gcattgccgg taatcgaccg aaatcctccg gcgtcagggc gataccgtca
    40741 ttctgatgtt tcatgctgtc cgcatggagc acatttttac cgctcatcgc cagcaggcgg
    40801 gccggtggcg ttcccgtccg gctttccacc gcctgcgcaa tgctttcggt cataaagccc
    40861 agcgtgcgga tatcgttacc gcctcgccgg gttttcatga tgttttttgc ccagatgcgg
    40921 aacgccagct gccgctccgg gctgttgttc atctcctgca ctaccatttc ccgcagcgcc
    40981 gggcttttca cctcgatcag tttacggatc aacgcctggt ctgtgccgaa cgccgccgag
    41041 ccggggttat atgaccagcc cacatccggt gtcatggttc tggtgccatc tgaatatgtg
    41101 gtcaccggca tttctctgac ttctccggtc tgcttatcca cgccagcctc aacattgcgg
    41161 gtggaaagat gatcctgacc ggatgaaacg gataacccca ttgcatccag acgggtctga
    41221 gacaatggcc ggacacggca gcggcagttc cagccattgg gtgggtagtg tgtattccag
    41281 aacggatcgt catagcggaa tacctgcccg ttaagggcgg aatgcgccgg gcgggtgcgg
    41341 ctgtccatca ccgccacata ctgccagaac ggatgcgtgt ccgtgttgtt catcatctgc
    41401 gtgtaacgcc cggcattgta agccacgcgg gtgttcacgt tgtaaatcag cgccagacgg
    41461 cggggactgc ccagttgcac ttcttctgcg ttaccggcgc tgtccaccac aatctgcttt
    41521 ccccaccatc ccagcttttg caggcggggt gtcagtgtgc ggataaatgc tttctgtgaa
    41581 atcccctcat caacagcacg ctgcacttcc gcctgtagtg tggtcagtac gtccaggcgt
    41641 gccacttttg ccgccgtgaa tgaacgggca tgaacatccg cgtctgtttc gaaccagttc
    41701 cagctgatat gcgccccttt ggcgcggaag tatgccaccg cctcttttga gggaagcgtg
    41761 gcggcataac ctaaatcaat cccctgtgcc atccagcatc cccttcattt ccgcagcaaa
    41821 catggcatca ctgagcagcg tcatcaggcg ggaatcatcc atttcacggt aaagggcagg
    41881 caggtctgcc agtgcctccg ccagcccccg cgttctgatg gcttcaatga ccggctccag
    41941 taccgggtcg atggcctcct gtaaccggcg ggcaggcacc gcatccccca tgtcgtccag
    42001 ttcatctcgg gggctgctct tcgcttccgg cagtctggca gccagcgctg tctgctctgt
    42061 cttctcctgt ttttccggtg gcatctcttt gtctgtctga gcaggttcat tgccactctg
    42121 acggacacgg aagatggctt caccgggggc aggctgtgga ataccggtct gctcccgcac
    42181 ccagggatca ggaatatcca tccccgtact gagctgcatc accgcactgg tgattttggt
    42241 gatatccccc ggctcttttg tctggaaaca gatacgcggc aggcgacgga tatcaatggc
    42301 gtgggcggtg ttcagggcat acagcggata caccagatcg cggttcagcg tggcggccag
    42361 ctgacgtaaa tcagaatccc tgatttcccg gcgcacctcg ttatgcactt cacccagcga
    42421 acgtgcgccc ttgtctccgg cttccgtggt cagcgtgccg ccgaggatag ctttggagat
    42481 ggaacgctcc ccccacgaaa tcatggtttc aaacggatcg gcctgaccgt tcgctgccgc
    42541 ctgaaactcc agcgacatcc cggccgggat gatcccgcct gtacgtcgcc cgatatccat
    42601 caccgcccgc atcagggcgc ttttctgctc cggtgttgcc ccggacgggt atttaccgac
    42661 cttcatcggc aggccgtaca cctccagaaa ttcagccaga tcgcgcacgg aatagttttt
    42721 gaaaatgaac ggccagataa gcgttctgac aagccccgtc gcaccaccgt agccagtgcg
    42781 tgaacgcgac tgatgcacta tccagccaaa gggctgaaac gccaccccgg catggctgcc
    42841 gtcacgcagc cgcagttcgc tcaaatcatc cgggttaagg cagaaatgcc cgctgtcacg
    42901 ccagcggatg gcgcggatga tgtgcatttt accgagcatc ccgtgctcaa tctccataca
    42961 ggaatagccc ttcaggatgg catcggtggc atcaaacagc atggcatcaa accagtcggc
    43021 ggaatgcaga tattcgtcga gcatttccgc gtcctttttc tcattcgcgc tggcgttcgg
    43081 gggcggctca atactccagg acaccccctg aatggcaagg cgtcgcttgc ccagctctgc
    43141 aaaaaggtgg gtatcctttt cttcaatgtc agcggccaga tcggactggg caatcagatc
    43201 gccacgttca gctccgcgaa ggcactgtgc cgcccggttc ggggtgatac ccgaggccgg
    43261 atgttcaata taacggctgg caatctgcgg aatatccagc acggcactct gcatctccgg
    43321 gtcaaaggag aaaggttttc cgtcaagatc aattatgcgt cccactacca gcacccccga
    43381 tcaaattcat gatatgcctc atcgtcatca cgataaccgc cctccattgc ccgcgaccgc
    43441 tccggcagcg cctgacaggc tgattcatcc aggatgaagc cctccatgta tgacgcccgg
    43501 tttgccatac agagcgccac ggcaaaatca ccatggcggc gggcgttcac agccgttgcg
    43561 ttctggtcct tcgtacgccc cttgtcgatt tgcgggatac cgttcaccac cttcacatgg
    43621 cgcaaatcat caagcgtggt ctgatggcgg gcgacgagga tattctgatc ctcaaactcg
    43681 gccttcagtt ttggcatcca ctcgccatac cacttcggcg ataacatcac gcagtcgatg
    43741 atgtccgggc caaaggccag cagcgcggct tcggccagat agccgccgtt accggtggcg
    43801 tcgaacgccg caccgaccag cgccggaacg cgggtcagga tgtacatcat cacctgctcc
    43861 tgctgggcat acggcaggtt gcgcagctcc acgcgaaaca cctcgcgttt tgccagggat
    43921 tcagtgattt ccagcagcac aaagcaggac aggtctccgg tgcgggcaaa gtcttccccg
    43981 aagctgaagc gggaacgggg atttaacgcc tctaaaagcg gttttaaatg ttcttcacac
    44041 caggttaaaa cctccgattc acgcagccat gcggcacggc tgatgaagtc gtccggggct
    44101 tcaaacgtca gaatcgggat gtcgcggatc atcgccattt caataagcgc gtgaggaata
    44161 taggcaccgc cggatttttt cgggatacag ccgtattcct cgtcggcatc ctcacgggtc
    44221 ggggcgtttt tgtagagatc atcacgccac ttctgctcgc tttccggtga ccattcgcgg
    44281 ccggtgacat aacagatacg acggtacagc ccgtccgcaa tggcatcatc cagggtaatg
    44341 cggtggacgc tgtaatcctt gcgcccctcg cgggcttcct gaatgtactg attaaacaga
    44401 ttatcgacgc cgttatgcgt ggagataata cgcacgcgcg cgccccacat ggtaagcgcc
    44461 attgccgcct tgagaagctc atccagtgac tcgtggaacg cggcttcatc aatcaccaca
    44521 tccccctgaa ggccgcgcag gtttgacgga cgggaagaca gtgcctgaat tttgaatcca
    44581 ctgttcggaa agcggatcat gtaggtcaga atttcttctt ttttatcccg atcccagaag
    44641 gtctgctcat acacatcagc atctgccagc tggttaaagg cacgggagaa cagtgcgcag
    44701 gcagaaatat attccagcgc catctcctgc tttgacccca cataaaacac attgcggcca
    44761 ccacgccgct tcggttttgc agcggtaatg acgttacgcc cggcttccgc ccaggtaagc
    44821 ccggtgcggc gggatttttc cgcgatgcag acctcgcttt catcctcaaa ccagcgggcc
    44881 tgataatcca gaaacaccgg catatcaccg ggcaaatcaa ggctgtccgg cacatccacg
    44941 cccagcaggg ctttttcacc ggccagatca attttgcggg tggagacggt attcgtcagt
    45001 aagggtgaga gttcttcggc gttattcatc aggctttacc caacagaatc cccttaatac
    45061 gggactcaag ctgttcactc atcccgtcca cgccgcgcag ctcgtcggtg accgcgtttg
    45121 ccatctcttc agcaaatgcg gcccggattt ctttttcacg cttaatgctg cgctcggcgg
    45181 cactttccgc acgctgtgct gacaggagga tgtctttgat gaggcgcata tccacgtcgc
    45241 tgtcgctgtt cagagattcg gtcgcggcac gcaggcggcg gtacatcagg gcgcgggaca
    45301 tttccagaat cagcgctgtg gtttcaccgg ttggcttgtc ccccagttcg gccatcatgg
    45361 cttttgtctg ctcgcgtaaa tcacgcaggt tacgggcaat cagctcatta cgggaggctt
    45421 cccggctgat ggccgccggt gaaagctgct gctcttccgg caggcccgcc tctcggatca
    45481 gacggttgat ttcctcgcgg atctggacct gcgtcagacg tttttcacgc agcatttcca
    45541 gcagcggctt acggatgctg tcggggagca aatccacctt gcgtacacga ccacgcgtcg
    45601 gcttgtccat cgttaccccc ttgcgcgtgg tttcttcaca cccggaacgc aggcccggcc
    45661 ttccgccaca tcctgcccac gaccggtcag ttccgcgatg aaataaccgt taatcagaac
    45721 gcgtttgcgc accagtccct gctcggcaag ccaggcaatg tgggtgtgaa cggtgtcgcg
    45781 ggacacgcgg tggccgtaat catccagaca gtcctggagc acggattcac ccagttcacc
    45841 gttgtaatct gccagcgacc gcagaatgac cagacgctga tcttcagtaa tgaaatcact
    45901 cattgcgttt ttttccttac agcctgctcc agcagcagtt cgttctgata ggagacagag
    45961 cggagcgtgg cgttcgtggt tttcagttca ccccgcaggg tcacgatttc cacattcaga
    46021 cggttgacct cggcttctgt cggcagtgac gacaggcggg tttccacctt ctcaacgcgg
    46081 tcggagagtt tttcaaaggc ctcgcggggg acaaacgttt tgcgcatcag cgccatgaat
    46141 atcccgccag cggttgccgt cgccgaaagg atcggcacaa cataatcttt aacgatgctg
    46201 acccacatta cgggcctcca tgatgttctg gcagttcaca caacggatgg catcaggcac
    46261 agcaacaaga cgtgcggcgg ggatctctcc accacagtcc gcgcagacac gtctgcccgg
    46321 cgtatccact gcctggcgct tacgtgtcag ccggttacaa agggcgcgtt ctgatccccg
    46381 ctccatcaca gcctgagcac gatctgaatc atccatacct tttccttact tcgtttttcc
    46441 tgccggaaca gcaggcgttc cggccatatc agtaaattca tgttgtcgcc gcgtctccag
    46501 acgacgaata ctggccttgt ccacgttgca gttcttgatc accgccagca gttgcaggtt
    46561 gtaagtgacc gatgccccaa acgtgaacgg ctccggcgca ggagggacta ggcagtcagc
    46621 cagccattca gcggggatcg gcaccggctt cacaggcacg tatttcacag acggcccggc
    46681 gcacccgatc agccacatca tcaggaacag gggcagaggc cgcaggcact gctgccagcg
    46741 accggcggac aacgctctgc tgcccctcgc tgtgctgaat gttctgttgt ttttcattcg
    46801 tggccgcctc actgatttgt gaaatgagtt gcagggtgtg ctgctgattt gccagcacct
    46861 gacgcgcctc atcccgctca cgcacaagca cctgattatt gtcttcaagg cgctgcgtct
    46921 tctggtgcat tccccatatc gcggcaacca gtacagcgat aatccccagc gccgccatgc
    46981 acgccccggc aatcagggcc ggtaaggcat tattcaccgc agccacagga caccccgtaa
    47041 cagcagtacc gcacacagca gcgctatcat gatcggtcgc cagaagcggt tcagactttc
    47101 ggcaaatcgt agcgacataa ccattcatcc gccgaacggc gggcctcaag gccggggagc
    47161 ttcaccccct ttgaataaat ccagcggata tactgcttac aggacgcggg catctgcccg
    47221 gcattgatca ggcgcagaag cgtggagttg cgaaagttcg tttcaccggc ccagaagatc
    47281 cacgaagcca gcgccaccgt ctgaccacgg gtaagcggca cctgaacacg ccgatcaatg
    47341 gccgcaaagg cccatttcat gtccttttcc agtagttcca ggcattcttc atccgtcagg
    47401 gtcatacccg gtttcacatc cgggccggta tggccgtaac agatggtggg tgtaccggtg
    47461 ggatcgatat acgtggagtt ttctttccct tcccagtaac cggtgtaatg cgtggcaatc
    47521 gtgaacgtcc cggcaccggc cagaacaagg gcaatcagct ttttacgcag tggtgcgggg
    47581 agttttggca ttgttacgct gtctcatgat gttctctgag tcagcgtaac ggtgaaggaa
    47641 aggcggcagg ctttgcggaa tatcagaaaa gtggcggttg tgttcttgcc agatgcagac
    47701 ggcgctgttc acgtatgacg gtataaatct gggtttctga catccggtaa tggcggcgaa
    47761 gggattcaat tttctcgccc cggtcccagc gcgaaaatat ctcattgttt cgcagctcgg
    47821 caaagagaga ttccccgacc ggcaggtaat aaccccgtcc gcccatatag gctgcctgag
    47881 ccgcagccac cttgcgtgcc agtttgccgg ccagtaccgg ctcaatcccc tgacgctgaa
    47941 gttctgcgcc ggtgacttca accagttctg acagggtccg cggccagttc tttttgagaa
    48001 catcatcagg gatgtcatca aggcggtcga taagggcgtg cagttgctca ctgtcaccac
    48061 caaacatgct catctgagtt tctgccatat cagcctccgg ttctcgcgtt cccggtaagt
    48121 ttaaaataaa aatcccgcgt tgtggcggga tttggggtta acggggaagt gatttttcat
    48181 actgcctgca aacggcgtca taccccatca gcgtgcgttc gggcattccc atcgcggcca
    48241 gcattttgcg gcggtgccat cgcttgagac gctccagcac atctgaggcc agggcggggt
    48301 gttgctccag ccactgccag tttgccacac cttcgccgcc gttttgcgcg gccgtctgcg
    48361 atttcaccca gcggttaagg gcggtttccg caccatcaga gataaagccc tgccggtgca
    48421 tcaccttcca gatggcgcga attttagcgg tcaccgtacc cggttttaag gcccggttaa
    48481 ccgggttctg acgcacttta aaaccgcgtt ttttgaaaac atccagcacg cgggataact
    48541 catccggtga catatcccgg cagctggttt tgccggtgac cgccagtagt acggatgtgt
    48601 aggtttcatc atccagggca agctcgcgtt ttgccacatg aatggcagat atcagtttcc
    48661 ctctcatgct gtatcaacac tcctgcggat atgataggta ttattttccc gatcgagctg
    48721 aacccgatac tccggaggaa tcacaaaaat attaaaacca tcatcagttt ttattcttga
    48781 gtagcattcc cccttatgat atctggccag tgcattaatc agagaagtcg cagcctgccc
    48841 aatcaggtat tttttatgaa aaatactgat aactttccgg cacagccatc gtgtaatgac
    48901 gaatgcaccg aaaaaggatg tgatatataa ataccagaac aactgcgaac tactgtccat
    48961 tatctttctc cttattcagt cttctgcggc attcggcaca gtcatcaggg ctttcaaatg
    49021 tatccggctc gcgattatga gccagatata accaccgtcc acatatactg atattttcgc
    49081 ctgttttaaa aaaatgatgc ttcctggcca ttagtggccg cgcccatcca gcgggtagac
    49141 tattcatttg ctttatcctc catgctggac agaatcatat gtattaatgt cgggcgtgga
    49201 gatatacgca tttcattaag cgtctgaagg gcagcaacag ctgcctgttt atcggtgcct
    49261 ttggcaattt cagcaagaga atgcacaatc cataatgggc ttaacgctac ggctgaatgc
    49321 agaatcatgt catccataat aatttctggc atatcccgtt tgtcacattc tttatccata
    49381 aaagaaacga ttttttctgc atcttccggt ttcatgcctc cggcgatgag aattttacga
    49441 tattcataag acgatcttaa ttcaatactc atcattcacc tgcctgttta atcagtttcc
    49501 agtgctttgc tacgggcaac agcctcacac tcaaaaaata ccgactccgg cacacgctcc
    49561 tttaattcac gaacaagaaa atcattaacg agttcatgac gtcttatatt tctttccttt
    49621 tcctcctggc gcaatacagc aaggcggtat tcaacagtcc ggcgttgttt ctgaaagaca
    49681 gccagtgctt ttctggcaga gttacgcaat gtatcatcgc cacagggttc gtattcaagc
    49741 tgaaatttaa tctcttcaat ggtttctttt atttttgcca gcaagctaag acagtcgctg
    49801 attgtcttca gtttttcatt atcaatataa cttctcacgc attcacctcc cgtcattccg
    49861 gtaaagctga aatatggcac cagtgcgtca cctgtccgtc ctcatcttca aggcaggctc
    49921 cggtttcttc atcaataaaa aagccttcat accccatatg gccgatgcgc agttcctgtt
    49981 ggtcaccata ctgaacaatc agcacgacag cctccatatc aggcgggaaa gcatcattca
    50041 gtgaatgcca gggataaccg cgcaaatccc cgaaataaac accggtcagc gttccgccac
    50101 agaccggcat tccgacggca cacacaaggg gttcatctga ttcaacaaat aatgtcgtta
    50161 tcatggcatt aattcccgta atacaacatc ttcggcagtc cgacgacggt gataaataaa
    50221 actcaccgtg ccctcggata attcaaattt ctcgccgatt tccctaaagg taagacgccg
    50281 tggtgattca gcatcccgta attcacgaat aagacgaaca tcatcgtcag gtatgcgggt
    50341 gaagggcagc aactctccgt attttttcat gcttacgcca atatatcgcg cccgattgtg
    50401 gacagaatcc cggtctcgcc ccagatattc tcctatttgc ttaccggtca ttgttctggc
    50461 attttttcgg ataaatttat cttcatcagg tttaaatctg aggcgtttgc atttgagcaa
    50521 ctccgggtaa gtaccgcgta ataaaacaat ccggttatgt acaccccaga aacttctttt
    50581 tatcctgaca gcaatatcct taaccggggt ggatggataa agcgcaatca gcagcgcatc
    50641 ttcttcaggt gtccatgcgc gaacatgagc gggtgcacgg ccttttctcc ccatgggttg
    50701 taatatcatc atgcatcacc ccctgactcg cttttcagca gcataaaact catcagacag
    50761 aatttcggtc attttctgat ttgcgggttt tacgccgcac tcaaggtaaa tcacatcgtc
    50821 aacgcagaac cacttcacag ggccaaacat gattgccgag aaatcaatac ccagccagaa
    50881 caacagtgca tcggttctgg catacgtgac gggcgaatat tcacgccaca gactgttaag
    50941 ttcgtcagag gcaacgcgca gggctttggg aatacgtgat gtgcgcggtg tgcagctcca
    51001 gccattcgac gcagtcggtt ttcgccataa gtcgcgatga aaaggatatt tgtcatccat
    51061 aaagcgcagc cctttaaaac aaaagccact gataccggat acaaataccg accggcacgc
    51121 aacattcagc acggcctcaa ggcgttttgc ctcatcctta actttctggc agtcctgctg
    51181 atatttttgc cacgcggcca gcgcggaagg gtttgatgtt ttaaagaaca ttacgccacc
    51241 tccgcagata tcccggcagg cgcggccgtc cggtcaacaa tcaggtaacg cagaacgcgc
    51301 gaggtgatat tccagccgct taaaccgcag ataatgacac ccaggcgtat gtcgatatac
    51361 gccatcacaa actggggcac gccttctttc atggcctgct catcaacttc agccaccaca
    51421 taaacggttt cacaggtcag aacgccctga gcaaattccc acgccattga cggaatatca
    51481 tgcccctcaa tccacggcag ggattttctg aagctgcacc actgaacatc atcagccgca
    51541 ccttcctggc tgcatttgcg acgaaccggc tgcaccgggc ggggacgcgg aatatcataa
    51601 aaaccgttac attccgtcag caccccggca tcgaccgcat cacgcagaaa ataaaccatc
    51661 gaggatggcg gcatattcat tttttcagcc agaatgccgc aggtcagacg cccgtaaata
    51721 ctcagccagt ttttaacccc ttcaagcact tttgcatcaa tcatgaatta ttcctccgtc
    51781 agcgccacat attcagaatg tttaatctcc tggcattctt caggaatacc cccttttatt
    51841 tccctgccat aagcgcctac tttaaacacc acgcacaggg catcacggga gagcgacggg
    51901 gtccaccagg agtccatgtt aaacatggag gggattctca ttacccccag cttttcacag
    51961 agccagacaa ggaaatcagg acgctttctt aactcgcgtt cagcggcttc aatcattgcg
    52021 gcaatcgccg gtccttcagg gcatgactca tcaggtctgg caataaagca cggtccgtcc
    52081 ccgataaaag ccacatcatc gcgcttacgc cagcccgcag gcagagcacc agaaaaataa
    52141 aactcactga ttacgtccat ggcaaacggc tttaagcgca atccggcagc gcctgtattt
    52201 ttcagtaatg cccgaagaat actgttgcgt agcggtaaaa cagtctgttg atatttggtg
    52261 aaatatttat ttgtctgcac acggtctaat ttgaaataaa aatatgccat atatcctgtt
    52321 ccttattttc agtttacagg cgcaggcagt cccctgacgc gagcgccata attaaaacga
    52381 agtgatattt aattgattaa tgcggtgtta tttattcagt ccggcgtcct gttcaaaagg
    52441 ctcgacataa aaactttcag cgcctttatt caccttaata ccggcaatcc ctttcaccgc
    52501 atccggctcc gccaggaccg cttccttgtt cacttcctct ttcgtgcgga taaaacgctc
    52561 aaggcccata cggcgcagca tttcaatcac accttccaca tcacgactga cgctgcatga
    52621 tggattcccc agtcgccatg acaccgttcc ggtggtcaga ttggcagttt tggttttgcc
    52681 gccgttcgtc agctcatcac ggttggtttt gcaccagtcc tgaatcccct taaaaagcac
    52741 tttgatttct tttttaaggt tttcaatctg cggcgtataa cgggcggtga tttctgccac
    52801 ttcatcattc atcgctgttt ccaggcgcag cgcctctcgc tgaatatcgc ccagggtgcg
    52861 gatatcgcgg ctgacctctt cacgggtctg cggtgccgcc tctgctgcgg cctttaattt
    52921 tgtaacgcgt tttgccattt tgtttttcct tattgcagtg tgcctgatgc tttatgcacg
    52981 cggtggcgtt taatgatggc atccgcccca aaagcctgta cagatgcctg aaattccgct
    53041 gagagaaact gaataatttc aggactgtga tactggagaa tacttgcata aacgtcacag
    53101 agtgacgtcg gcctgttatt cgtatcacgt tctgttttta catccacacc aatcttttca
    53161 tgcacttcaa cacagtcatt gcctgccggt ttttcgtcat gcgaaacatg cttaaattca
    53221 aaaataacgc gtactttgct catggaatta cccctgctga ttaaattcat tacctttaat
    53281 aaccccggaa cgcatgctca tatctttcat gagcgcactg gctttcattg ccacaagttc
    53341 agcaaagtct ctgctttccg cgagcataac caccccaaaa atcatggcgg gtccatcacc
    53401 tttctgcttt atcccggttg aacgtatgtc cagacttacc cccatgcgca ctgttccatc
    53461 agcgttttta tggataacgt gctccttgta ttcgaaaata atgcgtacga ctttactcat
    53521 cctctggtcc tcgttctgtt tggataactg acccggcgtc tgacctcacg gcagaaccga
    53581 atcatggcat taaaggcttc ttcctccgtc accgcttccg gtatccccgg aaccagcaac
    53641 tgctcctgtt catcacgtcc atgacgcgcc ataacctcaa tcacatgccg taccgtggca
    53701 ggctttccgg caactatcgg caatgcacct tccggcagga cgtaaccaaa ctcaataaga
    53761 ccactcgacc acgcccatgc aatgagattt tttttcacct cacacctccc agtaaaccgt
    53821 gcagccgctg atgcgggtgg ccttcacacg gcggcgcagg ccgttggtct gtatggtgat
    53881 ttcaatttca ccggttccct gcacgttgcc taccggcggc gtggttttca gtgcaaaacg
    53941 ttgcggatag cgcttactgc gttccagtac cgcaccggca agttcggtca gacggcgcgc
    54001 gctgttcagg gagtcgaata aattcatttt gttaccgcag gattgcatat tcatataaca
    54061 cctcatttag ctgacttgtt tcgcaccaac agaacggcat attccgctgt aaacgtctgt
    54121 cgcaccttcg cgatcaagtc cggcagatat caccattcgt gtgcggtcat taaattcaaa
    54181 aagtaaatcg ccacattctt tattttcggc cagtgaaatc accttcacgt tatcgaaatt
    54241 aaccaggtaa aagcgtccgt aaatatcagg aatattaaat accgccataa tcacacctgt
    54301 gagagtaatt cagggttggt ataaacctct ttaaaggccg cattaatatg tttttctgtc
    54361 agcgccgcgc cttcaccgct ggcggtgagc cacgcctggt taagcgtatg tgtcagaacg
    54421 cgtaacgctc ccggcttttc agcgatagcc tgcatgacgg ccagctcggc ctcaccactg
    54481 atcccccacg ccctggcaat ggccagcaca tccgcctttt tggccttgcg aagttgtttt
    54541 gtacgggcaa gacggctgaa caggcgcgat aaatcatcaa aggcgcggcg tccaccttta
    54601 aacaatccgc gcgggttacc aataagcacc atcccgatcc ccgtggcgtc ctgaattgcc
    54661 cggagttgct ccagaccgtc aatacccaga tgatccgcct catccacaat caccagtcca
    54721 cgcgttccca tcaggcgacg gcggatggcg cgggataatg cccctttgtt cgcgcgggtg
    54781 taatcaatcc ccagcgcatc ggccagctcc agcagacact ccgtgacgct ggagtgcgcg
    54841 ggtgacaggg tgatcatcca ggtgtttggt tgctcctggc agtaattacg ggcagtggcc
    54901 gttttaccca cacccggtac gcccacaata acgttaatac agcccatcag acgaaccgcc
    54961 tgaaacagtg cgcgcagctc ctggactgtc tgcgtttcca caaactgcgg cggttccggc
    55021 agtgcgcttt gtttattcca gttttcatac caggaacgca gggaagcagc cacagcggcg
    55081 ttatcgcctt tatatttccc cttgcggaaa gccgataatg tgccgtcgga aattcccgcc
    55141 tctctggcga tggcatactg cgtcagtacg ccgccatcaa taagttcatc aatggtcttg
    55201 attacatcgt taatatcagt catattatta acctctcatt tgataccttg tttaatcaaa
    55261 taacctgagt cgccgctcgg gttatttttt tatttcaggc cagcgggtca ttttctttta
    55321 atttcgcttc cagcaactgc aatccccgct ggaaattacg ctcgtattct tcatcaggtt
    55381 catcgtcagc gaccggttgc tgaacggtca ccgtattacc cacagggcgg tatatgtttt
    55441 ccagccaggg ctcctgcggc ttgtgctcca gcacgttgac aacctcatcc tcggcatcac
    55501 ggattttttc ctctgcgcgt ttacgcatgc ctttaaggcg ttgctgctgt ttgtagtatt
    55561 ccgcgctgac cgggaaggct tcgcgtttat tgccgtccca taccgcctcg caaatcacgc
    55621 tgccatccag gcgacgtacg gtaattcgtt cggcatcatg aatgtcatag ctgataagca
    55681 ccttacggcc atgctcgtca cgcagctcgg gcgcgtagta aatattattc agccagcgta
    55741 tttcacagcg tcttacaggg cgttccacca tcggccggaa catatcccgc agctcaacat
    55801 cggacagcca ttcaatttcc gtgtcctctt ccgccaggcg tttttttctg aactccgccg
    55861 ggctgtaatg tttaccgttc ggcttcatcg gtaattcatc gtgcggccgg ttgttgtacc
    55921 actcaacacc gtcacgaatg gcatcaatca gttcagccca ggacggtaaa tcacgcatcg
    55981 ctgactgttg ccgggcgttc agccgtttgc cctgttgcag ggcggtaaat gccgagcgta
    56041 aatcgcggtt ggttttgcgt aacgtctcgc gatctgcacc tttcccgaaa taggtgcggt
    56101 atttacgggc tatgcgcatc ggtaatgtgc ggttaagccg ttcgataatg cctcgcccct
    56161 gcggattacc ggcaatcccg gtcgggtgat taatccccag tcgcggcagt atccccacaa
    56221 cctccttatc caggacgtcg gcggtttccc cggagccgtt atccgagtaa tacagaaacg
    56281 gtttgccgtg atggcgaatg ccgtgctgta tggcaccggc tacggcgaaa acattttcag
    56341 ccaggtcaag gctccagccc accacaaagc gcgtgccacc gtcgataaca aaggtcactt
    56401 ccggtgcaaa tggccgaccg tgaaccgggt gtgcgcattt cagcttcatg ccgtgaccgt
    56461 caccaatcca gacataattc accggcattc tggaccagtc gcggcgcgtg aatccctcaa
    56521 gctggcggta ttcactgccg gtcacccggc ctttttgttt caccacttcc ggcagtttct
    56581 tcattgcgcg gcgaatggtg tcataagacg gcatgatatc gagcatataa ggctcatcag
    56641 cgtgccggtg ctgccattca gcgacaaaat cctcgtaagc ctcggtcatt ggtcggccgt
    56701 ttgactggcg atactgcgcc agaaattcgg gcagccagtt aatatcttcg gcttttattt
    56761 cctggcgctt acccggtgcc agtaaaagca ggcgttcagc ggcgttctgc gccttgttaa
    56821 aggccgcaat ccagcgtttc agcgttattt cactcaacgc acggctgttt ccctttttgg
    56881 cgttcgccgt ctcaaccatt gccacaatgc gctcgtccag ttgcgaacgt gccaggttgt
    56941 caacgataaa ccggatagct ttcgcacagc tgaaaccggg gtgttgcgcg actttcatca
    57001 cttcgctgac gatcgcgatt cgtgcatcag ccacctgacg ctggttttca gtcagggcat
    57061 taaggcgttc aaccatcagt tgtggtgatc cgcgatatgc ctccaccgca tcaaccacgg
    57121 caggcgagcg tctggccttt gtcaccaccg gagccggtga ttcatcagct ttttgcgtca
    57181 tcagttgaag ggcataacgt tcacgcaata cttgttgcgc gccagaaggc aaacaatcga
    57241 tattaaattc gattgctttt gtccccttac gtttgcgtaa catggctgca tttcccgcag
    57301 ctgcttttgt gagtcgatgg cgaatgttat gttccatcat tggtaaatca ggaagaccaa
    57361 cacattcttt tgctgttacc cacatacatc ccccatcaag ccacttctct ggcgtccacc
    57421 tggtatctgc tgggccagat tacagcaggt tcaaccccta ttttttcggc gatggcggct
    57481 tcgtatttac ggcactggcg gtaaaacaca ttgcgcattg atccgctttt taatcctaac
    57541 tccctttcca gttcagggag gtctgtgcct ttgttttcaa gggctgcata taccgcttta
    57601 gcagaccagt cacacccctc tcgaacaaac aaattattcg atgcttgtct ggataccctc
    57661 attgtgtgat cctctcatgt ttacctaaac caataatcaa aaccggttat cgatttaggt
    57721 aaacgatact cgaacaataa tccgagatca agctttttgt tcggtttatt gtttggtttt
    57781 tcttttgtgc gtttttgtag gaaaaccatt tttatgacaa atagttatga agaaaaaacc
    57841 gatgtgaaaa gggaaaagtt agatcggatt attagccgga gcacaataaa ccgctttggt
    57901 gaaaggctca aagtggccat gaaagggatg agtaatgccg aattagcgag acgaagtggc
    57961 atgtccgaaa caacaataag gaaataccta cagggaaaga tttaccctgc acttgatagc
    58021 ctggctattg ttgctgacgc ttgcggggta tctctgactt ggctcgcgac tggagaacaa
    58081 caagcaaacc aaacaatagt tcgagatgat gctaaacaaa attatgacaa tgagtatgaa
    58141 ttactcattc acttacttca taagctcaaa aaagaagaat gtgtattact ggttgatttc
    58201 atatcgcgtg agggcgtaaa cacccttata agattggtgg caatgaatgc ctctacgatc
    58261 agccaggatg ccgtagaaag catcattgat acgttaccgt tacgtccagt gctaaaaaat
    58321 gcaataaaag taggaatggc gggaagcgaa gacactgaca aagagatttt gcgcgttatt
    58381 gagaagtaca actcgagcaa taacgccacc caggaatcaa gggcagtgac agtaaagaaa
    58441 aatgttgttg gttaagcgcc atcgctatca tgaggtggtg ctgtaaatac atatagagat
    58501 catatgactc atcccaagat aatcacgcta ttgaatagta attcatgatg attatcatac
    58561 tgattatagt tagatcgatt atgtgagcat tttggcagtt tgaagatgtt ttgaagaaga
    58621 ttttaagggt aaattagcag tatcaaatgt gtcgccgttt tttgatcttt tgactatttg
    58681 ttgagcagtc ggtatcaaaa agagttttcc gtccgaacta tacatcacct gttttatttc
    58741 agcatcttac aggtgatttc acctcctttc actaaaagcc ttgtcggtat caaatgattc
    58801 acctggttac actattttgc cgccttcctg atgtaccgct tcggtaatgg tgcgatggtg
    58861 tgggatctgg ctggcgtcgt tgagcattgc gccaccttcc atgccaacgc ctgttaaatc
    58921 tggtgcaatg ccgccgctga caatcagcgc cacgccgtga cgggcgcgtt cggcataaaa
    58981 cgctgccagc cgctcggcac cgtccgggta ttcctccagc ccggtgtgca ttgagcccat
    59041 caacacgcgg ttttttaacg tggtaaaacc taaatccagc ggggcgaaca gcaacgggta
    59101 gctcataaaa ttgtccagta tgtaaaataa ttgttatgtg gtcggatgag ttctaattta
    59161 gccgtcgccg gagaaaaggg aaaaggggag tgcggggttt gtgatgggat tcaaaaagaa
    59221 tacgcccggt ggtaacgccg gatgcgacgc ttgccgcgtt ttatccggcc tgggggattg
    59281 cacatgtagg gcggataagg cgttcacgcc gcatccgcca gtggcacggt gcagatgccg
    59341 gatgcgacgc ttgtcgcgtt ttatccggcc aggaggattg cacatgtagg gcggataagg
    59401 cgtttacgcc gcatccgcca gcagttgcaa attattgctt acggaaaaaa tcgttataca
    59461 acatatacaa atgcgcggca ctccaggaga aatttggtgc gccttgctgt gcgccagtca
    59521 gcgggttgta attctcctga attgggccat cggcggttaa tcctttggcg tgacggaaga
    59581 acgtatccgc cagtttcagg gcatcatcac gataaccgta acgctccatc cctttcagac
    59641 caaaccagaa ctgatccacc catacgcgcc cgcgccagta gatatcagcg ccaaaagccg
    59701 gattggttaa cgccgccgtt cccagcggga caaaggtgtt gaactcttta ggatcgagca
    59761 tcaccttcac caccgcgtcg gcgttggcct gcgttgccgc accgttaaac agcggcgacc
    59821 agccttccgg ccctttaccg cgctcgacaa tcggtttgcc cgcgcagccg ttcgccagcg
    59881 gtttatcttc aatacgcacg tcatagtaga actgggtagc cgggtcgaac atacaggtgt
    59941 ttatgtagtc tgcgagctgc tgtgccaact ggcgatagcg tttggcctct tccggcttac
    60001 cgaggatggt tgccatctcc gccagataat ggttatcgct gtacatatag ctggcctgat
    60061 ccaccgactc ctgcaatagc gagtagccca gcaacgttcc gtcctgactg cggttttcgg
    60121 cgaatttcac cgtccagtcg ctacgtttgc cgccattagc gacatattta tccaactgtt
    60181 ctttgtcgat aaaaccaaag acggcggcat catcacgccc cgattcccac gacgcagcga
    60241 cctgtgccgg aatttccaga ctgtcgtact ggcctttctc caccacgcgg gcgtagttgt
    60301 tcagcccaga ctgcgtctct tctttgttgc ctttcttcac cgtaaacagc atctcgccgc
    60361 tctcagtgtt gtgggctttg tcgcgggtcg cgccatatta cggcacgccg ttgccgttgt
    60421 gatcgcggtt acgtaaccac cagtcgtgat aggccaccag tttcgggtac atctctgcca
    60481 accaggcttt atcctgggtg acgttgtaca cttccatcac cgaccaggcg gcaaggctgg
    60541 gtttggtatt acgttcgttc cagttgccgc catcgccacc acgctcgggg ctaaggttcc
    60601 acgctatcag gtcggggaca aagcccacat cctgcggacg cacgctatcg ccaggctgga
    60661 tctgccagga gaagaccgcg cggatattct ctttggcgat gtccggattg aaatgcgcca
    60721 tcgcaaacgc ctgcttccag gtatcccacg gccaggtctg attgccggag aaccagcgcc
    60781 cggtcaccga cggtgtaacg gtgttaaatt tcaccgcacc gcccggtgag cgccagttac
    60841 cgttgagcgt ttcgatcgct ttcaccgcga cgcgcgtctg ttccggcgtc gcatccggat
    60901 tggttaaccc tttcttcaga tactcttccc agcgttgctg cgaggcggtg agataaaacg
    60961 ccggacgcgc cagaatatcg cggatctgca tttgctcttt gctaacttcc tgagaggtca
    61021 gcagatggga ataggtggta tagagcgtcg tcgaaccgtt gatatgtgcc ttactggtaa
    61081 agcgattgcc gttgatttca gtctgcaccg gcagggattt atgcacctga tattccgatt
    61141 cgccggaggt cagcagatcc caggtggcgc gcactttgcc aaaggtgact ttcaggccat
    61201 cacgggtggc gctgattttg cgctggtagt cagggtattc gccagcaatg gttttatcgg
    61261 aaagcggttt cccttctttc gcttccagtt tttccagcag ttcgccgtcc cacaccagat
    61321 ccagcggttt attgctggtg attttggttt ccagtagtga cgtgcgcggc gtggcgaagc
    61381 gcagagtcat ttcgacttgc acatcttttg ctgtcagttt ttgcaccagc gcaccgggaa
    61441 tactgtatgc ctccagcgta aagtcgactt tcttgccgtc ctgccagacg gtcaggcggt
    61501 cgaaattgct ggccataaag ttgatgtact cttccgtcag cagcgcaacg cccggaaagc
    61561 cgcccatggt gttagggccg tctggcaaca gatgaccatg ccaggctccg agatcgaaaa
    61621 acggattaaa acgctggtga tcgtcgtaat cgtaatcctt catgtactgc ggcgcgccag
    61681 tacggttaat cacgttttta taattgtcgg cgttagcggc atgggcggaa aaacttatca
    61741 gcagagcgca ggttactggc gttaaaatag tttttatttt catgaaatta cagtccttat
    61801 taccagtaga aatattgcat caggaagatt tggtcgctgg gatcgtgaga ggcatttttc
    61861 atgtagaagc ggctatcaaa agccgtggcg aaacgtccgc cgccgtattc gtaccagata
    61921 ccaaactgca tgaaagaggt ggtttcatcg tcagcgtggt cagggcgatg tttggcgtag
    61981 gtgtaagcgg tggagagata aacgcccttt aacgcttcat acatggcgct caccatcatc
    62041 ccactttcac ttcccggtac gtccagcccg tcggcgcgtc cggtatggtg ccaggcgcga
    62101 gcggcaaagt taggagttag caggcccgtg agatagagct gtcctgtgcc gtcggtgatt
    62161 tccagaccgt tcatccaggt gatgccttcg gcaaggtcat actggatgta accgttcatc
    62221 attgccggat aggtgtattt gtcgtcatag cgatcgtact tgccaaaatg tagccaggct
    62281 ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa gttgccgctt
    62341 aagttctgca ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac gtagcccaga
    62401 tcgggggtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg acgcagactg
    62461 agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt aattccgccc
    62521 tcgaaaccac cccagctatg gtctggcacc caggcattgc cgtcgtggtc ggcctgcacg
    62581 gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc caggccacct
    62641 tcaatatagg taccgtcgct gtatttatgt ttattctcac cctctaaatc catatatcca
    62701 ccaaccccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt aattgcaggt
    62761 gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga aaaggcgtag
    62821 caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat tgtgttaatc
    62881 catgagctaa agaaaaaata cagcctttta tcgttgtaat tataaaaata taaaggcaga
    62941 aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt tttattattc
    63001 tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag ccgttaatcc
    63061 ctttatatat ttactgtatt tccaccacgc aaagccaagg aagataacaa taccgccgac
    63121 gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag cgacaaaccc
    63181 tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc gggagcccat
    63241 acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag ccagcatgat
    63301 gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatagtat tcatcagatc
    63361 ctgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga tcaggaactg
    63421 gatccacgct gcgcgcgccg gaacgccgtt ttcgttcagt tcgacggttt tcttaccaaa
    63481 gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc acatcagcag
    63541 ggaaccgaac atcgcggtaa aggacaccag accgacaaag cgattcatca acgcttccgg
    63601 tagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgacgg taaatttcaa
    63661 ctctttgctg ctgacgaaga cgttaatcag caccgaggag acggaataca gtacgccgat
    63721 aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt tgacgtcgtt
    63781 aacgtacaca gcgacggact ccgcgccacc tgcggccata aagatccagg tggtaacgcc
    63841 gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatggggt cagcaggctg
    63901 tacgccgcca accagcgccg tacctgccag taaaatgtag gagagcgtta acagcagcat
    63961 cagcgttgaa gtgacggagg taattggccc caacattttc gccccgttgg tggaaaccca
    64021 ggtggagaag gcgaacagca ccatactgat aatggtggtg gcaaccggcg tcataatata
    64081 ttcgtatccg aggaaggcat acgaagcata agcaataacg cgcggcaaca gtgaggtgaa
    64141 aaagaacagg tttacgaacc agtaggtata ggcagtaata aatgcccaac gtccgcccag
    64201 cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg aaacaaattc
    64261 tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa acatcggtgc
    64321 cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt aaacggcggc
    64381 aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat cagacataac
    64441 acatctccga aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg ccgtagttgt
    64501 tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt tttgaccgcg
    64561 ttattgcaga taaaccgata cgcttcatgg atatcgcaaa taacgatttg cccttcgtgg
    64621 acctcaacgg tttctccgca gccttttaaa tattcacggt cagtttcatc acgataatat
    64681 tccactacct gtaatgtctc tttcggtgca tattcaattt tttgctgccc ttgcaggtag
    64741 taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc ggagtctgtc
    64801 tccacgcggt aagtcaatga gtcaccgatg gagtgggcga cgccaggctg aatgttgtcg
    64861 atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta aatctggcgg
    64921 aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa ttcgtggaaa
    64981 agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct ccgccagaaa
    65041 ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag gagtccagca
    65101 cctcgctgcc ccaggagttg gagccgaggc caagtagctg gtggtcgagg ttcagggtga
    65161 tgtcatcact gcgctgcagc tcgttacagt gctgggcagc gtggatgttt tcctgggtat
    65221 agtgccaggc gctgaagtta attgggcgct gcggaaccac cagcagaccg ttgccgtggc
    65281 ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgc gggaagggat
    65341 agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta gcctgctggc
    65401 tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctgatcg tattcgccgt
    65461 taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg taacgctcgc
    65521 cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag gtgcagcgca
    65581 tcccgaagtc aaacaccggc ggggcaataa ctgtgcggct gatgatcaac acttcaccat
    65641 cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa tgattcggtt
    65701 gccacagccc ttcgttctcc tgcttgtggt tgtcgatcat tggcttgaag aagttgatct
    65761 ttggctcgcg ggtcagcagc gactcgccat tcacctgcca ggatgtcggt ttgccactca
    65821 tttttgagaa ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa cgatcgtctt
    65881 ccagcgtcag cggacgcgca ttatttggtg cgaaaggcac tggttgcgcg gtgttttcct
    65941 tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg gtgcgggaat
    66001 ctttggtcac cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc agcgtgatct
    66061 gcaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc gtcgcgagcg
    66121 tttcaccttc gacgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg gtaaaccaca
    66181 gtttattttc gactttcagc tcgccgcgag tcagatccag cgcgtggatt tttaccggcg
    66241 cgataacctg tttgtactct ttcaggcccg gtcccggcgt ctgatcggaa tagatcaaac
    66301 catcaagaca gaaattatag ttgttgggat agtcgccgta gtcgccgccg aatttatacc
    66361 agacgttgcc gttgtcatcc tgcgcctgga tcccgtggtc gcaccactcc caaacataat
    66421 gtccctgaat acaatcgtgc ttatagaaga cgttctggta ctccgtcagc ccgcctggtc
    66481 cgttccccat cgcatgagca tattcacaga tgatgcgcgg cttcggatgc gggtattcac
    66541 caaactcatt catcagcggc acgcgggtgt acatggtgga aataatatcg accacttcag
    66601 catcgcgatc ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgccttc gctgcgtggt
    66661 acatcgcgcg gatgttacag ccatagccgg attcattgcc cagcgaccag atgatgatcg
    66721 acggatggtt tttctgcgcg tgaatatggc gaacaatgcg ctcgacgtag actttttccc
    66781 actgcggatc gtcggtaata cggctgatat cgccgacatt agcaaagccg tgcgattcga
    66841 cgtcggtttc cgccatcaca aacaagccgt agatatcaca cagttcgtaa aaacgcggat
    66901 cgttcgggta gtgagcggta cgcacggagt tgatattgtg ctgcttcatc aactggagat
    66961 ctttctcgac gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg tcgtgacggt
    67021 tgacgccgtg cagcatcaca taacggttat tgatccagaa cagaccgtcg cgcactttga
    67081 tatcacggaa gccaacgcgt tgtggcacca cttccagaac gttgccgttg gcgtctttca
    67141 gcgtcatgac cagatggtaa agataagggg attctgctga ccattgctgc ggctgttcga
    67201 cagtaaaagc aaagctggcg ctggtcagtt tttcaattgc caaatgatca atggcgctgc
    67261 tgtgcaccac gcgttcgcca tcaaacaagg tatattccag cgtcgtgacg acaggggagg
    67321 cggcgagatt ttccagcacc acttcgcagg aaagcgtggc atcgcaatag gcttcgtcaa
    67381 agtcggtacg cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc agataaacat
    67441 cgcggaagat ccccgctgac caccacatat cctggtcttc cacgtaggta gagtccgccc
    67501 actgcatcac gcgcacacac aacaggttgt cgccggtttt aaccatcgcg ctgatgtcaa
    67561 actctgcggt caggcgactg cccttgctga aacccacata ctgaccgtta acatagactt
    67621 caaaataggt ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc cagccgtcgc
    67681 tgagggtgaa aatacgttga taggcaccgg ttgggttatc gctggggaca aacggcacat
    67741 cgatggggaa cggaaaacct tcgtcggtat attgcagttt gccgtgacct tccatttgcc
    67801 acatggcggg gacggtaata tgcccccagt cagccattaa ctcagaggtg aaggcttctg
    67861 gtacttgcag cggatggtca aaaaagtgga aattccactg accgcttaag ggcagaaaca
    67921 ggctgctggt ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat gaaaaaaagt
    67981 acgcacgcgg cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag cgattcatag
    68041 ggcctcccgg tagagagaaa tcggaaccat tcatggtgat ggtagtttgt gtaattgctg
    68101 gggagtttag taaaattttt actaaaaagt agcaggtaaa tgcatttact ttaactggat
    68161 cacgaaaata aacggcagca ctgtccggca aaaacgattg ccggacagtg tgtaagggga
    68221 tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactaac agcggcagcg
    68281 cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg
    68341 aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa
    68401 cgctgataag cgaaatatcc tgtgggatgt tcaggcctcg ttcatgaatt gcccgcagta
    68461 cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca
    68521 gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct
    68581 cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg
    68641 ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat
    68701 agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc
    68761 tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc
    68821 gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta
    68881 agccgctgtg ttcataacag ttggtgagct cgatgcccag cttttcgcac tgggtttcaa
    68941 tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt
    69001 agatagccag aatatggtgt tggttgactg cacctgtctg gagtttacgg gcactactgg
    69061 tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat
    69121 tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga
    69181 ttgcgatgtc ttttagtgtt gccatgttta ccttttttac tttccctgat gtgcgccccc
    69241 attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga
    69301 cgctgtaacg gtaacacagc ggctcacgaa tgtgatcacg cctgcattct ccatttttag
    69361 cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa
    69421 acaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt
    69481 gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg
    69541 gaaaaaacac tgcaaaccgt gaccagcctg gtgccgtact acagcgaaga agagctgatg
    69601 gggaaaacgg tggtggtctt atgcaatctg caaaaagcga aaatgcgtgg tgaaacgtcg
    69661 gaatgcatgc tgttgtgtgc ggaaaccgat gacggcagtg aaagcgtgtt gttaacgccg
    69721 gagcggatga tgcctgcggg cgtacgcgtc gtataaaaag atcggatggc gacgtcgtat
    69781 cgccatccga tttgatatta cgcttcttcg acacttactc gcatggccgc cagcgcctta
    69841 cgcgccgctt tgatcaccag ttcacactgt tcaatggtca gtgtcagtgg cggttcaatg
    69901 cggatcgttt tggcgttatt gagcgttccg gccaccagta cgcgctggcg gaacatctcg
    69961 ctggcaaagt tatagccgat ttcgttatca acaaactcaa ttgccatcaa catcccttta
    70021 ccacgcgctt cctgtaccag atcgggatat tcccgcgcca gttgacggaa accgtccagc
    70081 aacatatcgc ctttttgctc agcctgagcc ggtaagttct gctccagcaa cacattgatg
    70141 gtcgccagcg ccgccgcaca ggccagcggg ttgccgccaa aggtggtggt atgcaggaat
    70201 gggttgtcga acagaactga aaacacctct tcagtggcga tggtcgcgcc aatcggcatc
    70261 acgccgccgc cgagcgcttt ggcaaggcag aggatatccg gctgtacgtt ctcatgctcg
    70321 caggcgaaca tcttgcccgt gcgccccatg cccgtttgta cttcatcgag gatcatcagt
    70381 gcgccgaact catcgcatag cttacgtacg gcggtgagat agcccggcgg cggcagaatt
    70441 acgccacctt caccctgaat cggttcgagg atcaccgcag ccacatcatc accggttttt
    70501 ttgcactcgt taagagccgt gcgcatggct tcgatattgc caaacggcac atgacggaag
    70561 cccggcagca gtggcataaa cggtttgcgg aaggtcgatt tcgccgtggc cgacagcgcg
    70621 ccaagtgatt taccgtggaa cgcgccgctg gtggcaataa aagtaaactt gccgcgcggt
    70681 gactggtaag ctttcgccag cttcagcgct gcttcaacgg actcggtgcc gctattacag
    70741 aagaagctgt atttcagttt accgggcgtt agcgcagcaa gggttttcgc caacatcgcc
    70801 cgtaacggat cgagcagctc ctggctgtgc agcggttgtt tcgcaagttg attctgtacg
    70861 gcggaaacca caactggatt acggtgcccc acgttgaaaa ttccaaaacc tcccaggcag
    70921 tcgataaact cctgtccctg ggtgtcgaca agcgtattta aacctcccgc ttgccactct
    70981 acggctccgt aatccccgcc ggcggtaaca gatttgcgat actctaaaaa ccccggattg
    71041 acatgctctt tgaagtattc aatcacctct cggttaagtg ctttcatctc ctcatgatcc
    71101 agcgttcgct tctcaatgag attcagggcg tgggcgctgc acgctaaagc cgatgcgctc
    71161 gaaggtaacc tgttcaaaat atgctccgga ggctcgcgta tcacatgata ccgatttaag
    71221 tattgcaggg attgcgccac tccggctgcg atcgcgaaaa tcgggataaa cacaaattcg
    71281 gttatcattt gtgcaatatt taatgctatc tgttaacatt tgttgttatt ttgcacggtt
    71341 tttgtcgggt atggatttga tgattgctgc gcaaatgcac gttttcaggg caataattgc
    71401 ctgatttttg ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa gtattttaaa
    71461 tcaaagaatt agatttgtgc tttaatcgca aattgcgatc taaatcaaat taatcggtta
    71521 aagataaccg cagcggggcc gacataaact ctgacaagaa gttaacaacc atataacctg
    71581 cacaggacgc gaacatgtct tctcatccgt atgtcaccca gcaaaatacc ccgctggcgg
    71641 acgataccac tctgatgtcc actaccgatc tgcaaagcta tatcactcat gctaatgaca
    71701 cttttgtgca ggtgagcggc tttaccttgc aagagttaca agggcagccg cacaacatgg
    71761 tgcgtcaccc ggatatgcca aaagcggcgt ttgcggatat gtggttcacc ctgaaaaaag
    71821 gggagccctg gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat tattgggtgc
    71881 gggccaatgc ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg tcgattcgta
    71941 cccgggcgac ggatgaagag attgcggcgg tggagccgct gtacaaagcg ctgaacgccg
    72001 gacgtaccag taagcgtatt cataaaggcc tggtggtgcg taaaggctgg ctgggtaaac
    72061 tgccttcatt accgcttcgc tggcgggcgc gtggagtgat gaccctgatg tttatcttgc
    72121 tggcggccat gctttggttt gttgctgccc cggtggtgac gtatttcctc tgtgtgttag
    72181 tggtattgtt ggcaagcgct tgttttgaat ggcagattgt gcgcccgata gaaaatgtcg
    72241 cccgtcaggc gctgaaggtg gcgaccggag agcgtaatag tgttgagcac ctgaatcgca
    72301 gcgatgagct ggggctgaca ttacgcgcgg tagggcagct tggcctgatg tgccgttggc
    72361 taattaacga tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag acgctggcga
    72421 aaggcaccga tgaactgaac gaacataccc agcagacagt tgataacgtt cagcaaacgg
    72481 tggcgaccat gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg gcgtcggctg
    72541 ccgataaact ttctatcacc gccagtaatg cggcagtgca gggtggggag gcgatgacca
    72601 cggtgatcaa gacaatggac gatatcgccg acagtaccca gcgcattggc accattactt
    72661 cgctgattaa cgatattgcg tttcagacca atattctggc cctgaatgcg gcggtggaag
    72721 cggcgcgtgc cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa gtgcgtcatt
    72781 tagccagccg cagcgctaat gctgccaacg atattcgcaa gctgattgat gccagtgctg
    72841 ataaggtgca atccggttcg cagcaggtac acgccgccgg acggacgatg gaagatattg
    72901 tggcacaggt gaaaaacgtc acccagttga tcgcccagat tagccattca acgctggaac
    72961 aggccgatgg gctttccagc ctgacccgtg cagtggatga gcttaacctg atcacccaga
    73021 aaaatgccga gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa caccgcgcca
    73081 gccgactgga agacgcggtg acggtgctgc attaatcggt atgccggatc cggcggttgg
    73141 agcacaatgc ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg tgtagcgggt
    73201 gtaggccgga taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc cactggcgga
    73261 tgcggcgatt aatccagttg cgtaatatca aaagcggcgc ggtcgatcac cgcaatgatc
    73321 ttttttattt gtgcatcact gatatcgctc tggttgacgt gtaaatccag caccgcttta
    73381 aaattatcca gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc aacgcaacgc
    73441 gctttgatgc gttcttcaat catctccacc tgttcgcggt tttcttccag ccactgcgcg
    73501 ccttgttcgg tcagcgcaat ctgcttctta ccaccttcct cttcgcggat ggtaatcagc
    73561 gactgctcct gcagaaaatc cagcgttggg tagatgacgc ccgggcttgg ggtgtaattc
    73621 ccctgggtta ggttctcaat cgctttaatc aattcgtaac cgtggctgtc atcgcgcgag
    73681 agaatatcca gaatcaccag acgcaattca ccgtgaccaa agaaacgttg ccgacgaccg
    73741 ccgccgcgac cgtggcgacc accgcagcat tgaccgtgtt catgctggtg accgtgtccg
    73801 cagtgctcgt gttctgactt ctcacctttg cagcagccct catggcgtgg ctggccttca
    73861 tgtttacaac acccttcgtg atgatggctc atatcagcct ccttttgctt gattagatat
    73921 atctaattta tatctgatgt gagcattact gcaagtacaa aaatgatttt taaacgtgga
    73981 agcacctcac atccaggccg gataagacgt tcacgccgca tccggcagca ttgctatggc
    74041 actgaatgcc ttcacatact tcagatatat cagttctaca aatcgcttgc atttatcatg
    74101 attaacaatc attatcattt acgaaaatca tttagatata tctgattact atcacgaagg
    74161 cgataacaat gaataactcc ccccgctacc cgcagcgcgt tcgcaatgat ctgcgcttcc
    74221 gtgaactgac tgtgttacgc gttgagcgca tcagcgccgg ttttcagcgc attgtcctcg
    74281 gcggcgaggc gctggacggt tttacatcgc gcggctttga cgatcacagc aaactcttct
    74341 ttcctcaacc tgacgctcac tttgtgccgc caacggtaac ggaagagggc atcgtctggc
    74401 cggaaggacc acgcccactg tcgcgtgact atacgccgct gtatgacgaa ctacgccatg
    74461 aactggcgat cgatttcttt attcacgacg gtggggtcgc cagcggctgg gcgatgcagg
    74521 cgcaaccggg cgataaactt acggtggcag gtccgcgcgg ttcgctggtg gtgccggaag
    74581 attacgcgta tcagctgtat gtctgcgatg aatccggaat gcccgcactg cgccgccgcc
    74641 tggaaacgtt gagcaaactt gccgttaaac cgcaagttag tgcgctggtt agcgtgcggg
    74701 ataacgcctg tcaggattat ctcgcgcacc ttgatggttt taatatcgaa tggctggcac
    74761 atgatgagca ggcggtagat gcgcgtctgg cgcagatgca aatccctgcc gatgattact
    74821 tcatctggat aaccggcgaa ggtaaagtcg ttaagaattt aagccgccgc tttgaagcgg
    74881 aacagtatga cccacagcgg gtacgtgcag cggcttactg gcacgcaaag taactgatat
    74941 ataaaaagaa aaaggctgac gatttctcgt cagcctttgc tatatctggt ggcccctgct
    75001 ggacttgaac cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaagggg
    75061 ccatggtagc ggattataaa gtaactccgt gtcgcaatcc agccattacc gtgcgcctgc
    75121 tgtttttata aacaatgtat tttcaatccg ttatactttt cctggtgatg ttagaaagga
    75181 gtaaatatgg ttgaggatat tttggctcca gggttacggg tcgtgttttg cggtatcaac
    75241 cctgggcttt catccgccgg gactggtttt ccctttgctc atccagcaaa tcgcttctgg
    75301 aaggtgatat atcaggccgg gtttaccgac cgtcagttga agccgcagga ggcacagcat
    75361 ctgctggatt atcgttgtgg cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat
    75421 gaagtttcaa agcaggagct gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat
    75481 tatcagccgc aggcgttggc gattctgggc aaacaagcat atgaacaggg attcagccag
    75541 cgcggtgcac agtgggggaa acaaacgctc accattggtt cgacgcagat ttgggtgctg
    75601 ccaaatccca gcggtttaag tcgcgtttca ctagagaaac tggttgaagc atatcgcgag
    75661 ctggaccagg cgctggtagt gcgtgggcga taaaaaacgc caccgaaaag gtggcgtttg
    75721 tgcaaaggga ctagtgccgg gtgcggcgta acgcctgatc cggcctaccg attaatcgtc
    75781 caggaagcta cgcaacactt cagaacggct cgggtgacgc agtttgcgca gcgccttcgc
    75841 ttcgatctga cggatacgtt cgcgggtaac gtcgaactgt ttacccactt cttccagcgt
    75901 gtggtcggtg ttcatatcga taccgaaacg catacgcaga actttcgctt cacgcgcggt
    75961 caggccagcc agcacgtcgt gcgttgccgc acgcaggctt tcggtggtcg cagaatccag
    76021 cggcagctcg agggtggtat cctcgatgaa atcccccaga tgcgaatctt catcatcacc
    76081 gatcggcgtt tccatggaga ttggctcttt ggcgatcttc agcactttgc ggatcttgtc
    76141 ttccggcatc agcatacgtt cagccagttc ttccggcgtc ggttcacggc ccatctcttg
    76201 cagcatctgg cgagaaatac ggttgagttt gttgatagtc tcaatcatat gcaccggaat
    76261 acggatggtg cgcgcctgat ccgcgataga acgggtgatc gcctgacgga tccaccaggt
    76321 tgcgtaggtg gagaacttgt aaccacggcg gtattcgaat ttatcaaccg ctttcatcag
    76381 accgatgttg ccttcctgaa tcaggtcaag gaactgcaag ccacggttgg tgtatttctt
    76441 ggcgatagaa ataaccagac gtaagttcgc ttcaaccatc tctttcttcg cacggcgggc
    76501 tttcgcttca ccgatggaca tacgacggtt gatatcttta acctgctcga tggtcaggcc
    76561 ggtttcttct tcaatctgct gcagtttctg cagggcgcga tgcacttctt cagagacatc
    76621 gtgcagtttt tccgaccacg gcttgttcat cgcaattgcc gcgttgaacc aggtatcgct
    76681 ggtttcgttg ccggtaaaca gggtaatgaa gtttttcttc ggcattttgc actgctcaac
    76741 gcagagcttc atgatcagac gttcttgcgt acgaacgcgg tccatcatga cgcgcatgct
    76801 gttgaccagg tagtcaaact gcttcggcac caggcggaac tgtttgaata cttcagacag
    76861 tttcaggatc tcttcctgag cggcagcgtg actgcgacct ttcgctttga tggtgtcacg
    76921 cgttacaacg tactgagcgc gtagttccgc aaatttttcg cgagccagtt ccgggtcgat
    76981 gctgttgtca tcatcggcgc tgtcgtcatc gccatcttct tcgtcttcat cttcgtcatc
    77041 gtccagatct tcctgggaaa gctcagaacc gacgtgagtg gcggtaggtg ccagatcttc
    77101 ttctgcgttc gggtcaacaa agccggtgat cagatcggac agacgcgctt cttctgcttc
    77161 aacacgatcg tactgttcca gcagataggt gatcgcttcc ggatattcag caacggagca
    77221 ttgaacctgg ttgatcccgt cttcaatacg cttagcgatg tcaatttcgc cttcgcgggt
    77281 caacagttca acggtgccca tttcacgcat gtacatgcgt accgggtcag tcgtgcgccc
    77341 gatttcagat tccacgctgg aaagcacctg cgcggcggct tcggcagcat cttcgtccgc
    77401 ggtgttttca gccagcatca gatcatcggc atccggtgct tcttccatca cctgaatgcc
    77461 catgtcgttg atcatttgga tgatgtcttc gatctgatct gaatcgacga tatcttccgg
    77521 cagatggtca ttgacctcgg cataggtcag atagccttgc tccttaccac gggtgacaag
    77581 aagtttcagc tgtgactgcg ggttttgctc cataagacgg tatccacact tatttcatga
    77641 ggttggtgtt gggcgattga cccgatgtcc ttcagccgtt aacgctgaaa tccaacggtt
    77701 gtttaccgcc aacgataatt acgagggcgt acttatatat ttgccgctgc ctctcagtgc
    77761 ggctgtcggg ggcttcccga tcgctcttcg gcacttaagc cgttaaatca ctttttcgcc
    77821 agctccaggt ttaatgtcca gagctccagg cgttcttcgt tgcttaaacc atgcgtgcgc
    77881 tcacgagcga ttaactcttc ctggcgcagt tcaagcagcg aatcaaacat atggttgagt
    77941 gagtcggtga aggtttgctc agcaatattc ttatctgcta tatcgtccca catcgacagt
    78001 ttttcaaggg tggcagcatt atttgtacca cgatagtgct ctaaaagttg cccggtggtc
    78061 agacctggct gggagagaca agtgttgacc agttctctga ataagccaag tccagggagc
    78121 ttattttcat ccagattctc aagcggcggg accaacgtcg ctaattctgg attttgcacc
    78181 agcaacccta taagtatacg catggtcgtg cgttttagct gcggaacagg gcgagaaacg
    78241 ccgctctctg ccgcttttgg cattaatcgt tcaagctggc tgtcatcaag tatgcctaat
    78301 ttgttgccta attcctgacg aagatatatt cgcagcgttt cgcccggcac ttgcgatatc
    78361 aatggcagtg ccagcgtact caaacgtgcg cgcccgtcag gggtactcag atcaacttgc
    78421 ggcatcaggc tgttaaacag aaatgcggag agtggcatcg cctgctccat ccgcgcttca
    78481 aacgcttctt taccttcttt tcgtaccagc gtgtcagggt cttcgccatc cggcaaaaac
    78541 ataaagcgta gctgacggcc gtctgtcatg taaggcagcg ccgtttccag cgctcgccat
    78601 gcggcatcgc ggcctgcacg gtcgccgtca taacagcaaa tgacattgtt ggtcgcgcgg
    78661 aacaacagtt gtatgtgatc ggcggtggtt gacgtaccta acgacgcaac ggcgtaatta
    78721 atgccgtatt gcgccagcgc caccacgtcc atatagcctt cgaccacaag cagacgattg
    78781 ggttcagcgt tatcctgctg cgcttcataa agaccgtaaa gctggcggcc tttatggaaa
    78841 atgtctgttt ccggcgagtt caggtatttg ggggtatcgt tgcccagcac gcgcccgcca
    78901 aaaccaatca cccgaccgcg tttatcgcga atggggaaca tcacccgctc gcggaaacga
    78961 tcgtaactgc gtccctgatc gttagtgacc aacatccccg catcaatcaa tgactggcga
    79021 ttttctggat tgccgccaaa ccgcttcagg acgttgtccc agccgggggg cgcaaaacca
    79081 atcgcaaagc gagcgataac ctcgtggctt aatccgcgtt tttccagata ctggcgcgca
    79141 gacgtggcaa caggttgttg taaagattgt tggtaaaacg tattcagacc gtccatcaac
    79201 tgataaagcg tttgcctctg atggcgctcg atctggctgg ggccgctgcc tgcttcaaat
    79261 ggcacttcaa gattgtgcat tgctgccagc tcttcgaccg tttcaacgaa ctcgagcttg
    79321 tcgtagttca tcaggaagtc gatcgcgttg ccgtgcgcgc cacatccaaa gcagtggtaa
    79381 aactgtttct caccgttaac ggtgaatgac ggggttttct cgttgtggaa tggacaacac
    79441 gcgtggaaat tcttgccctg ctttttcagc ttcacacggg catcgatcag atcgacgatg
    79501 tcagtgcgtg ccagcagatc attaatgaat acgcgtggga ttcgtccagc cataggcccc
    79561 gatttttagc aattcataaa cgaaaataag ccgcgcattc ctttcggaag cacggcctta
    79621 caactacaac tcggtctgat cggagagcaa cgctctcggg gaattagtac agacgagtgc
    79681 ggcgtgcgtt ttcgcgagcc agtttcttcg cgtgacgttt cactgcagaa gctttagcgc
    79741 gcttacgttc ggtagtcggt ttttcataga actcacgacg acgaacttcc gccagaacac
    79801 ctgctttttc gcaggaacgc ttgaagcgac gcagagctac gtcgaacggc tcgttttcac
    79861 gtactttaat taccggcatg tgcctctcac ctttgattaa ttcggtttgc cgctggcatc
    79921 aacgccagct tatttcaaaa tggtgcggaa ttttactgca attgctgctg ctttgtaaag
    79981 caccgcggcc ttttttgaaa gggactttta taagggtgag gagtatacac gaggctttct
    80041 ccaggggcga acaaagtttt acatcaaccc gcattggtcc tacactgcgc ggtaataaag
    80101 cgaggtaaaa caagtcatgc gtgtactggg tattgaaact tcctgcgatg aaaccggcat
    80161 cgccatttac gacgatgaaa aaggtttgtt agccaaccaa ttgtatagtc aggtgaaatt
    80221 gcacgctgac tacggcggcg tcgtgcctga actggcctcc cgcgatcacg tgcgcaaaac
    80281 cgtaccgttg atccaggcgg cgctaaagga gtctggttta acggcaaaag acattgatgc
    80341 tgtggcctat accgcaggcc ctggattagt cggcgcactg ctggttggcg caaccgtggg
    80401 gcgttctctg gcgtttgcct ggaacgttcc ggcaatcccg gtacaccata tggaagggca
    80461 tctgttagcg ccgatgctgg aagataaccc gccggaattt ccgtttgtcg cgctgctggt
    80521 gtccggcggt catacgcagt taatcagcgt gactggcatt ggtcagtacg agctgctcgg
    80581 cgagtctatc gatgatgccg ccggtgaagc gtttgataaa accgcgaagc tgctggggct
    80641 ggattatcct ggcggaccgt tactgtcgaa aatggcggct cagggtactg ccgggcgctt
    80701 tgttttcccg cgtccgatga ccgaccgtcc ggggctggat ttcagctttt ctggtctgaa
    80761 aacctttgcg gcgaacacga ttcgtgacaa cggcaccgac gaccagacgc gtgctgatat
    80821 cgcccgcgcc tttgaagatg cggtggtcga tacgttgatg attaagtgta agcgagcgtt
    80881 ggatcagacg ggctttaagc gactggtcat ggcgggcggc gtgagtgcta accgcacgtt
    80941 acgggcgaag ctggcggaaa tgatgaaaaa acgccgcggc gaagtgttct acgcgcgtcc
    81001 ggaattttgt actgataacg gcgcgatgat cgcctatgcc ggaatggtgc ggtttaaagc
    81061 aggcgcgacg gcggatctcg gcgttagcgt gcgtccgcgc tggccactgg cagagttacc
    81121 ggctgcgtaa aactatccgg tgccggagag caatttccgg caccgtcctc acttaaagca
    81181 acaccacggg catccacagc aagccggtaa tcaccagcaa cacgaggaat atcagtccaa
    81241 aaatcgcccc cagtcgccag taatccaccg ttggcagata accactaccg tagtaaatcg
    81301 gactgggtcc ggtggcgtat ggtgtaagaa tgctccccag accaattgcc gcgccaacca
    81361 tcaggcagaa caccggcagc gggatttccg gcatcgccag cgcagcggca atcatcatcg
    81421 gtgcgagggc ggaggtatac gccgtggcgc tggcgaaaaa gtagcgcagc agataaaaca
    81481 ccacaatcaa cgccaccatc accatcgttg gcgaataacc gcttaagctg cctgctaaca
    81541 gtttgccaaa ccagctaata aaaccagtgt tgttgagtcc ggtagcgagg gtgataagcg
    81601 aggccagcca gaagaaaacg ttccacgccg ctttattact gacaatgtcg tcccaactga
    81661 taatgcgcag aagcagcatc agtgccacca cgctgtaacc gaccatcgcg gcatcgatat
    81721 aatcaccgcc gaaaatccac agcaccagcg cgcctaccat cagccccagc atccgttttt
    81781 cacgcgaaca gagcgggccc attgcctgca gttccgtctc tgcccagcgc ggcacctgat
    81841 cgccagactt cagtaccggc gggtacagca cataagccag ccagggaacc agcagcacca
    81901 gcaaaatact gagcggcaac atcccgagga accagtcgcc ccaactcagc gtggcgtgag
    81961 atgcgctttt catcagtcca attaacagca agtttggtgc catcgccgtc aagaaaatgg
    82021 cgctggtcac gcagtcggca acaatcccca tccacatgat gtacgagcca atgctgcgcg
    82081 aactgctgtc gtttggttgt gattgataga gcggtggcag gttacggatg atgggataga
    82141 taatccccgc accacgcgcc gagttggacg gtgttacagg tgcgaggatc agctcggaga
    82201 acatcaccgc atagccgaga aacagcgtgc gatgtcccat ctttttcacc agaaacagcg
    82261 caatgcggcg tccaagcccg gttttttcat agcctgtgcc aaacataaag gcggcgaaaa
    82321 tcagccagat aaccgaatta gaaaaaccgg aaaccgccca cgagagggat tttgcagtga
    82381 atttaaagcc tggctgggcg agctgttcgg ggctgaacag cagccaggga gagagtatgg
    82441 cgatgatgga gatacccacc atcgccacca cggcacccgg cacgggttcg aggatcagtc
    82501 caacgatcac gccagtaaaa acggcaaagt agagccaggt atggttctcc agccctgcgg
    82561 gaaccggaag tagagcaata atggcgatga ccgccagcgg cgcaagatat cgccaccatt
    82621 cagtggaagg tttcatcgtg ttatgcctgc gaaaaaggag aggggtgagc ctctccggga
    82681 gggttatttg atgtaatgga cgtgctcgca gatctcttcc acgatgggat cgcggcgttc
    82741 ggcgaacagc tttttgtttt cggctatcag gttgttgccg tgggtatcaa tagagacaat
    82801 cagcgggccg aactctttga cccggcagac ccacagtgac tccggcatcc cgagttctgt
    82861 ccagtgcact tcttcaatct cttctacctg ggttgccgcc agcaccgcgc agcctgccgg
    82921 gaaaatcaca tgtagcgcct tgaatttctg gcagccttct tcggtcagcg gccccatacc
    82981 acctttgcca accaccagtt tcacgccggt ctgctcgata aattcacgtt caaaactttc
    83041 catacgcata ctggttgtcg ggccaacgga aaccatctcc catttatcgc cgtttttgcg
    83101 cacgatgggg ccagcgtgga aaatcgcttt gccgttgaga tcgtaaggga tcggacgttt
    83161 cagttcgatc aagcggcggt gacaaacgtc acggcaggtc accagcgtac cggtcaggta
    83221 gatcacatcg ccgacgcgaa tatcttgcag atcttcagct ttgatcgggg ttgtcaggat
    83281 ctttttcata acgcgctccg ggtgtgagac agattttcaa aggtgagatc ggcatgaacc
    83341 agcagtgtgc cgcgacgatg cgcccagcag cctgtagaaa cagcaacgcc gatggttgac
    83401 ggatggcggg cggcagattc gatatgcacg cccatcactg aactgttgcc agtcagccct
    83461 tgtgggccaa ttcccagacg gttgagtcct tcttccaggc gtagctccag ctctgccgct
    83521 tttggattgg gatggcggct gccaatcggg cgcaaaatgg cttttcgcga gagcacggcg
    83581 gcggtttcca ccgaggtggc gatgcccacg cccaccagta ccggtggaca ggcgtttacg
    83641 gcgagggtgg agatattttc gaagacgaat ttcaccacgc cttcgtagcc ttctgacggc
    83701 attaacactt tcgagcggcc aggtagcgtg cagccgccgc ctgccatgta aacttcgatt
    83761 tccgcatcgt cattgtcggg gatgatgtcc caggtgaccc acggtacgcc gctaccggta
    83821 tttttgccgg tgtttacttc gtcaaaaatt tctaccgcat tgtgacgtag tggcgctttg
    83881 acggttgcct cttccacggc ttgtttgagt atgctttgca gctcgccaag cagtgggaag
    83941 cgggaaccga ctttaacgaa gaacataatc tccccggtgt cctgacaggc aggacgattc
    84001 aggtcaatcg ctttttgcat gttgtcgaac atcgtatggt agataatttt ccccatcgac
    84061 gacgtttcgg catcctttag ctgttttagt ttatccacca cgtcatcagg cattcgggta
    84121 gaaatcatgg cggtaaagtt agcgacaatc tctgtcaact tattcactgc ctgttgctta
    84181 ttactttcgc tcatcatggc aactccagtt tttgtatttg tctggaaaaa ttcaccgcca
    84241 caaactacca ctaaaaatgt ggttttatat tttttgccac cgtgaatatg taattaacag
    84301 ttcgtgaatc aacgcgaaaa taagaatgaa tattgcgcca gagcagggaa tggcaatgtt
    84361 ttgcgaaata cactgcgctt atcacaactg cggataatta acgatgctga atagctggcc
    84421 tttagccaaa gacttgcagg tactggtaga aatcgtccac agcgggagtt ttagtgctgc
    84481 agcggcgacg cttgggcaga cgcctgcatt tgtcaccaag cggatccaaa tccttgaaaa
    84541 tacgctggca acaacgctgc tgaaccgctc cgctcgcggc gtggcgctga ctgaaagtgg
    84601 ccaacgttgt tacgaacatg cgctggaaat cctcacccaa tatcagcgac tggtcgatga
    84661 cgtcacgcaa atcaaaacgc gcccggaggg gatgattcgt attggttgca gttttggttt
    84721 tgggcgcagc catattgcgc cagccattac cgaacttatg cgcaattatc ctgagttaca
    84781 ggtgcatttt gaattgttcg atcggcaaat tgatttagtg caggataata ttgatctgga
    84841 tattcgtatt aatgacgaaa ttcctgatta ttatattgcg catctgttaa cgaaaaataa
    84901 aagaatatta tgcgcagcac ctgagtatct gcaaaaatat ccgcagccac aatcattaca
    84961 ggaattaagt cgtcatgact gtctggtgac caaagagcgc gatatgaccc acggaatatg
    85021 ggagttgggg aacggtcagg agaaaaaatc ggtgaaggtt agcgggcatc tttcctccaa
    85081 tagcggcgag atagtgttgc aatgggcgct ggagggcaaa gggattatgc tgcgttccga
    85141 gtgggatgtg ttgccgtttc tggagagtgg caaactggtg caagtattgc cggagtatgc
    85201 acaaagcgcc aatatctggg ctgtttaccg ggagccgctc tatcgcagca tgaaattacg
    85261 tgtctgcgtg gaatttctgg cggcatggtg ccagcaacgg ctgggcaagc ccgatgaagg
    85321 ctatcaggtc atgtagatcc accagaaatc actcgggatc cttttcgcgc tttcttttga
    85381 atttcgtcca gatttttgtc tcctgacgac gccacagacg ctggatgttg tcatgatgac
    85441 gcagcaggat caggcaagag agcatcgaaa ccgggaaggt gaattgtggc ttaaaccacc
    85501 atacataaaa cggagcaatc agtgcactga caatcgctcc cagcgacgag tatccgctca
    85561 atagcacggt cagtaaccag gttcccgcca ttactccggt gagatcccag ccaatgggtg
    85621 cgatggcacc aaaagcggta gcaacgcctt ttcctccttt aaatccgaag aaaacgggcc
    85681 agatgtgtcc aagacaggcg gcgatggcaa ttaagcctag ccagaagggg ctgacaccta
    85741 attcatacgc gccccagacg ggcaacattc ctttcagaac gtcgaaaatc agtactgcta
    85801 cggctgctcc cttgccaccg atacgtaaca cattggttgc gcctggattg ccggagccgc
    85861 tggttcgcgg atcgggcagc ccacacaagc ggcaaaccag aatggcactg gaaatggagc
    85921 cgcagaggta cgcgatgagg atcattccag gcgcgattgc actcataagc tgttccgttt
    85981 tgaaaattcg tgttcaacga tgaatctgtg gataatacgc acatttcgcc ggaagtggta
    86041 tccggttagc caaaaagcag gcaggacgtg atggatattg tatttataga gcaactttcg
    86101 gtaatcacca ctattggtgt ttacgactgg gaacagacca tcgaacagaa gttagtgttc
    86161 gatatcgaaa tggcatggga taaccgtaaa gcggcgaaaa gtgatgatgt ggcggattgc
    86221 ctcagttacg ctgacattgc agaaacggtg gtcagccacg tcgagggggc gcgttttgcg
    86281 ctggtggaac gcgtggctga agaggtggcg gagctgctgt tagcacgctt taactcgccg
    86341 tgggtgcgta tcaaactcag caagccaggc gcagtggcgc gggcggcgaa tgttggcgta
    86401 atcattgagc gtggcaataa tctgaaagaa aataattaat tttacagctg ttaaaccaaa
    86461 cggttataac ctggtcatac gcagtagttc ggacaagcgg tacattttaa taatttaggg
    86521 gtttattgat gagcgatatg cactcgctgc tgatagcggc aatattgggt gtggtcgaag
    86581 gattgacaga atttctgccg gtatccagca cgggccatat gattattgtc ggtcacttgt
    86641 tggggttcga gggcgacacg gcgaaaacct ttgaagttgt gatccagtta ggatcaattc
    86701 tggcggtagt agtgatgttc tggcggcgtc tgtttggcct gattggcatc cactttggcc
    86761 gcccgttgca gcacgaagga gaaagcaaag gtcgtttaac gctgatccac attttgctgg
    86821 ggatgattcc ggcggtagta ctggggctgt tgttccacga cacgattaag tcattgttta
    86881 acccgataaa tgtgatgtat gcactggtcg ttggcggttt gttgctgatt gccgccgaat
    86941 gcctgaagcc gaaagagccg cgtgcgccgg gtcttgatga tatgacctat cgtcaggcgt
    87001 ttatgattgg ctgtttccag tgtctggcgc tgtggccggg tttctcccgt tccggggcca
    87061 ccatttcagg tgggatgctg atgggcgtga gccgttacgc tgcttccgag ttctcgttcc
    87121 tgctggcggt accgatgatg atgggcgcaa cggcgctcga tctctacaaa agctggggct
    87181 tcctgacaac cggcgatatc ccgatgtttg ccgttgggtt tatcaccgct tttgtggtgg
    87241 cgctgatagc gattaaaacc ttcctgcaat tgattaagcg catttcgttt atcccgttcg
    87301 ccatttatcg ctttattgtg gcggctgcgg tgtacgtcgt gttcttttga ttgagaaaag
    87361 gttcgggagc aggcctggtt ttctccctcg ctctgtaggg agagggccgg ggtgggggtg
    87421 attgttcgca ccggtgctgg tctgttcccc tcaccctaac cctctccccg aaggggcgag
    87481 gggatggttc gtgttaacgt ttagttttat actcagtcag gctttgggca acgttgttcc
    87541 ttccaactgg ctaccgccgc aatccgccgt cgggtcagct cctcgcgaat ctccacacct
    87601 ttaaatcccg cttcaacgac cgcttttgtc ggcactgact gcgccacttc ccaggcttcg
    87661 cgcaaccaac ggccttgcgg gtagtccgct gattcaaaac cggttctgcc gcgtacgtca
    87721 gcctcgctgg tcagcgccag ttgctcgaca cgctgcggtt tacgccaggc atcgatggaa
    87781 tcaaataatt tgacgatggt ttttgggttc agcattggga aggtgtggat gagatcgtga
    87841 aactcagcca ccagtctggc taaatcgcga atttcatttg gcacgcgcag acgctggcat
    87901 agttgttcca ctaacttaac acccgccggg ccatgcccat gatgacgcgg ccaaagttct
    87961 ggcggcgtca gtcctttacc gagatcgtgg cataaagttg cgaaacggac atcaacctgc
    88021 ggactcagca tcgccgccat tgagagcgtc attaaggtat gaatacccgt gtcgatttcc
    88081 ggatgccacc tggcaggggc cggaacgcca aacagggcgt caatttccgg gaataaaacg
    88141 cgaagtgcac cgcaatcgcg cagtacctgg aagaacacct ggggattgcg ggtggtgagg
    88201 gcgctttccg tctctttcca tacccgttca ggcgtcaggt gttccagttc acccgcatgg
    88261 gtcatctcgc gcatcagcgt cagagtttca tcggcaatac gaaaaccgag gtgggcataa
    88321 cgcgcagcaa aacgcgccac gcgcagaacg cgtaacggat cttcgccaaa agcgggggaa
    88381 acatggcgca acagacgatt ttgcagatcg cccagaccgt tgtacgggtc gataatctcg
    88441 ccgttatcgt cctgagccag cgcattaatg gtcagatcgc gacgcttaag atcatcttcc
    88501 agcgtgacat ccggtgcggc atagcaagta aaaccggtgt aaccggaacc ggatttccgt
    88561 tcggtacgtg ccagcgcata ctcttcatgc gttagcggat gcagaaacac aggaaaatcg
    88621 cggcctacct gctggtagcc cgcgtcgagc atctcctgtg gcgtactgcc gaccaccacc
    88681 caatctctgt ctttgaccgg tagccctaac aatgcatccc gaacagcacc accgaccaga
    88741 taaatcttca cgccatcatc ccttcgtcaa atacacaatt cctaataata agacagtgca
    88801 acgaagaagg cgatttagtt catccagcga tctttgcgtt tgcgcgttgg gatcaggtgt
    88861 ggcagtacca gaccgagcag caagccaagc cccagcacgc caccgccata cataaaccat
    88921 tgcatgatga tggtgcgctg tttgtcatcc aactgtacgc tggcggcatc gacctttttc
    88981 tgcgcgacaa tcagctcgtt tttcaatttc tgattttctt cttttaatcc gttgatcacg
    89041 ctgtcgctct gcgccacttt ttgctgcatt tctgcggtgc gctgattcca ggtgttatcg
    89101 atattggtga gtttatcggt cagggttttg acctgatttt ccagatctgg cacacgggag
    89161 cgcaggcttg gctcagtgct aagttgtttc aacgggatcc aggcggtacg gccagagctg
    89221 tctttcacct gggcataatt ggtgttggcg tcagtttgta ataaggtcac ttcctcgccg
    89281 gcgttaaccg tgcccacgag gcgataatga tctcccggac cgctacggac ccaggtattc
    89341 agttcatcag aaacatagcg cgtttcttca gcgtgtgaga cggcagtggc gctaagtgcg
    89401 agtaaagtta atccgatcag gcgtaatttt ggcatcaggc tgtcgttatt gtcatggaaa
    89461 gtggaacgat agtagtggca tcagtgtcgc tacgcaaagc attcgacatc aatcggaatc
    89521 ctctgtgtca ggtcttgcca ctgcttcaaa cttttacgcc cgtcaaattg cccgttgcat
    89581 ggcaatttgg cgcaaaatac tatctactga caaaaaagat cgcattaagt tcgccgttca
    89641 tcgtcactta tgtgacataa ccgataagta aaggccatgg ctcaggaaat cgaattaaag
    89701 tttattgtta atcacagtgc cgttgaggcg ttgcgtgacc atctcaatac gctgggcggc
    89761 gagcaccatg accccgtgca gttgctgaat atttactacg aaacgccgga taactggctg
    89821 cgtgggcacg atatgggctt acgtattcgt ggcgaaaacg gtcgctatga gatgaccatg
    89881 aaagttgcag gaagagtgac aggcggctta caccagcgcc cggaatataa cgtggcgttg
    89941 agcgaaccga cgctcgacct ggcgcagtta ccgacggaag tctggccgaa cggcgaattg
    90001 cccgccgatc tcgcttcccg cgtacaaccg ctgttcagca ccgattttta tcgcgaaaaa
    90061 tggctggtgg cggtcgatga cagccgaatt gaaatcgctc tcgatcaggg ggaggtgaaa
    90121 gcgggggaat ttgctgaacc tatctgtgag ctggaactgg aactgcttag cggcgacacg
    90181 cgcgcggtgc tgaaactggc gaaccaactg gtatcgcaaa ccggattacg ccagggcagc
    90241 ctgagcaaag cggcgcgtgg ctatcatctg gcgcagggca atccggcgcg tgaaatcaaa
    90301 ccgaccacca ttttgcatgt tgcggcaaaa gccgatgtgg aacaggggct ggaagcggcg
    90361 ttcgagctgg cgttagcgca atggcagtat catgaagaac tgtgggtacg cggcaacgat
    90421 gcggcgaaag agcaggtgct ggcagccatt ggccttgttc gccatgcact gatgctgttc
    90481 ggtggcattg tgccgcgtaa agcgagcact cacttacgtg atttgctgac tcaatgcgaa
    90541 gcgaccatcg cttctgcggt ttctgccgtg acggcggtct actctaccga aacggcaatg
    90601 gcaaagctgg cgttgaccga atggttggta agcaaagcct ggcagccgtt tttagatgcc
    90661 aaagcgcagg gtaaaatcag cgattccttc aaacgctttg ccgatatcca tctttcccgc
    90721 catgccgctg aactgaaaag cgttttctgc cagccgttag gcgatcgcta ccatgatcag
    90781 ttgccacgcc tgacgcgtga tattgactca atactgctgc tggcaggtta ctatgatcct
    90841 gtcgtcgcgc aagcctggct ggagaactgg caggggctgc gtcacgctat tgcgaccggg
    90901 caacgcattg aaattgaaca tttccgtaat gaggcaaaca atcaggaacc gttctggttg
    90961 cacagcggaa aacgttaatc aggcaaggat aaaatcgctt atgaagccgc tctcttcacc
    91021 gttacagcag tactggcaga ccgttgttga gcggctgcca gagcctttag ccgaggaatc
    91081 acttagcgca caggcgaagt cagtacttac ttttagtgat tttgtgcagg acagcgtgat
    91141 tgtgcatcca gagtggctga cggaactgga aagccaaccg ccgcaggccg acgaatggca
    91201 gcattacgcg gcatggttgc aggaggcgct cagtaatgtg agtgacgaag ccgggttaat
    91261 gcgcgagctg cggctattcc ggcggcgcat tatggtgcgc atcgcctggg cgcaaacgct
    91321 ggctttggtt actgaagaga gcatattgca gcagctcagc catctggcgg agacgctgat
    91381 tgtcgccgcg cgtgactggc tgtatgacgc ttgctgccgc gagtggggaa cgccgtgcaa
    91441 tgcgcagggc gaagcgcaac cgctgctgat tttaggcatg ggtaagctgg gcggtgggga
    91501 actgaatttc tcctcagata tcgatctgat ttttgcctgg ccggaacatg gttgtacgca
    91561 gggcggacgc cgggaactgg ataacgcgca gttttttacc cgcatggggc agcggctgat
    91621 taaagtgttg gatcaaccaa cgcaggatgg cttcgtctat cgcgtggata tgcggctgcg
    91681 tccgtttggc gaaagtggcc cgctggtgct gagctttgcc gcgttggagg attattacca
    91741 ggagcagggg cgcgactggg agcgttacgc gatggtcaag gcgcggatta tgggcgatag
    91801 cgaaggcgtc tatgctaacg agttgcgtgc gatgctgcgc ccgtttgttt tccgtcgtta
    91861 catcgatttc agcgtgattc agtcgctgcg caacatgaaa gggatgattg cccgtgaagt
    91921 gcgtcgacgt ggtttgaccg acaatatcaa actcggcgca ggcggcattc gcgaaattga
    91981 atttatcgtt caggtgttcc agctcattcg cggtgggcgc gaaccgtcgc tgcagtcgcg
    92041 ctctttactg ccaacgctca gcgtcattgc cgcgctgcat ctgctttctg aaaacgatgc
    92101 tgaacaattg cgagtggcgt atctgttcct gcggcgtctg gaaaacctgc tgcaaagcat
    92161 taacgacgaa caaacccaga cgcttccttc tgatgagctt aaccgtgcgc ggctggcgtg
    92221 ggcgatggac tttgctgact ggccgcaact gaccggggcg ctgaccgcac atatgaccaa
    92281 tgtgcgccgg gtgtttaatg aattgattgg cgacgacgaa agtgaaactc aggaagagtc
    92341 gctgtcggaa cagtggcgtg agctgtggca ggatgcgttg caggaagatg acactacgcc
    92401 agtgctggcg catcttagcg aggatgatcg caaacaggtg ctaacgctga ttgccgattt
    92461 ccgcaaagag ctggataagc gcaccatcgg gccgcgagga cgtcaggtgc tcgaccatct
    92521 gatgccgcat ctgctaagtg atgtctgtgc gcgtgaagac gctgccgtta cgctgtcgcg
    92581 cattaccgcc ttgctggtgg ggattgttac ccgcaccacc tatttagaat tgctcagtga
    92641 attccccgcg gcgcttaaac atttgatttc tctgtgtgcc gcgtcgccga tgattgccag
    92701 ccaactggcg cgttatccat tattgctgga tgaattgctc gatccaaaca ccctttacca
    92761 gccgacggcg accgatgcct accgcgatga gttgcgccag tatttgctgc gcgtgccgga
    92821 agatgacgaa gagcaacagc ttgaggcgct gcgtcagttc aaacaggcac agctgttacg
    92881 catcgccgca gcggatatcg ctggtacgct accggtgatg aaagtgagcg atcacttaac
    92941 ctggctggca gaggctatga tagatgccgt cgttcagcag gcgtgggttc aaatggtggc
    93001 ccgctacggt aagccaaatc acctcaacga tcgcgaaggc cgtggttttg cggtggtcgg
    93061 ctacggcaag ctgggcggct gggagttagg ctacagttcc gatcttgacc tgatcttcct
    93121 ccatgattgc ccaatggatg cgatgactga cggcgagcgg gaaatcgacg ggcgacagtt
    93181 ttatctgcgt ctggcgcaac gcattatgca tctgttcagt acgcgtacct cttccggcat
    93241 tttgtatgaa gtggatgcac ggctacgccc gtccggggcc gcgggaatgc tggtgacatc
    93301 cgcagaagca tttgccgatt atcagaaaaa cgaggcctgg acgtgggaac atcaggcgct
    93361 ggtgcgtgcg cgggtagtgt acggcgatcc gcagctcacc gcgcactttg acgcggtgcg
    93421 tcgcgagatt atgacgctgc cgcgtgaagg taaaactctg caaacggaag tgcgggaaat
    93481 gcgcgagaaa atgcgcgctc atctcggcaa taaacatcgc gatcgctttg atatcaaagc
    93541 tgatgaaggc ggaattaccg atatcgaatt tattacccaa tatctggtgt tgcgctacgc
    93601 tcatgaaaaa ccgaagttaa cgcgctggtc agacaacgtg cgcattctgg aactactggc
    93661 gcaaaacgac attatggaag agcaggaagc gatggcgctg acccgtgcgt atactacgct
    93721 gcgcgatgaa cttcatcatc tggcattaca ggaattgccg ggccatgtgc cggaggattg
    93781 cttcaccgca gagcgtgaac tggtgcgggc aagctggcag aagtggctgg tggaagaatg
    93841 aagtatggta ttatcgcgcg caaattttga atctctcagg agacaggaat gaaagtaacg
    93901 ctgccagagt ttgaacgtgc aggagtgatg gtggttggtg atgtgatgct ggatcgttac
    93961 tggtacggtc ccaccagccg tatctcgccg gaagcgccgg tgcccgtggt taaagtcaat
    94021 accatcgaag aacgtccggg cggcgcggct aacgtggcaa tgaacatcac ttctctcggt
    94081 gccaatgctc gcctggtcgg gctgacgggc attgacgatg cggcgcgtgc gctgagtaaa
    94141 tctctggccg acgtcaacgt caaatgcgac ttcgtttctg taccgacgca tccgactatc
    94201 accaaattac gggtactttc ccgcaaccaa cagctgatcc gcctggattt tgaagaaggt
    94261 ttcgaaggtg ttgatccgca gccgctgcac gagcggatta atcaggcgct gagttcgatt
    94321 ggcgcgctgg tgctttctga ctacgccaaa ggtgcgctgg caagcgtaca gcagatgatc
    94381 caactggcgc gtaaagcggg cgttccggtg ctgattgatc caaaaggtac cgattttgag
    94441 cgctaccgcg gcgctacgct gctgacaccg aatctctcgg aatttgaagc tgttgtcggt
    94501 aaatgtaaga ccgaagaaga gattgttgag cgcggcatga aactgattgc cgattacgaa
    94561 ctctcggctc tgttagtgac ccgttccgaa cagggtatgt cgctgctgca accgggtaaa
    94621 gcgccgctgc atatgccaac ccaggcgcag gaagtgtatg acgttaccgg tgcgggcgac
    94681 acggtgattg gcgtcctggc ggcaacgctg gcagcgggta attcgctgga agaagcctgc
    94741 ttctttgcca atgcggcggc tggcgtggtg gtcggcaaac tgggaacctc cacggtttcg
    94801 ccgatcgagc tggaaaatgc agtgcgtggg cgcgcagata ccggctttgg tgtgatgacc
    94861 gaagaggaac tgaagcttgc tgtagcggca gcgcgtaaac gcggcgaaaa ggtggtaatg
    94921 accaacggtg tctttgacat cctgcacgcc gggcacgtct cctatctggc aaatgctcgc
    94981 aaactgggtg accgtctgat tgtcgctgtg aacagcgatg cctccaccaa acggctgaaa
    95041 ggggattccc gccccgttaa cccgctcgaa cagcgtatga ttgtgctggg cgcactggaa
    95101 gcggtcgact gggtggtgtc gtttgaagaa gacacgccgc agcgcttgat cgccgggatc
    95161 ctgccagatt tactggtgaa gggcggcgac tataaaccag aagagatcgc cgggagtaaa
    95221 gaagtctggg ccaacggcgg cgaagtgctg gtgctcaact tcgaagacgg ttgctcgacc
    95281 actaacatca tcaagaagat ccaacaggat aaaaaaggct aaccggaaag cggttcacag
    95341 atctcgcttc gtagcaggca ggcctattga cgctgttgtt ttttcaacct aaagtaaagg
    95401 aacaagggta agggaggatt tctcccccct ctgatcagtt gttagtaagt cgggaaactt
    95461 aacagtaaca acacaaccag tatgatgacg aacttcatca taaccctttc cttctataag
    95521 gccccttcct cgggaggggc tttcccgttt cagccccttg ctgactcccc gattgttgat
    95581 gagcgccgga ccccgcgcaa cgctttccct gaagcagttt tgccttcccc gagcggtcac
    95641 gcaaaacgct gcaacgtcct gagcatttgc accttttagc ggaacgcaga tataaaaaat
    95701 ccccggcttt ttgggccagg gatatgtgtt aatgaaacct tcttgacgct gatttttttt
    95761 caacctaaag taaaggaaca agggtgaggg aggatttctc ccccctctga ttggctgtta
    95821 ataagctgcg aaacttacga ttaacaacac aatcagtatg atgacgagct tcatcataac
    95881 cctttccttc tgtaaggccc cttcctaggg aggggctttc ccgtttcagc cctttgctga
    95941 ctccccgatt gttgatgagc gccggacctc gcgcaacgct ttcccagagg cagttttgcc
    96001 ttccccgagc ggtcacgcaa aacgttgcaa cgacctgatc atttgcagtt tttagcggaa
    96061 cgcagatata aaaaatcccc ggctttttgt gacagggacg ttcatagctg aaaatttatt
    96121 ctacgttttc ggcgacggga gttgttgagg ttaagggtga agtcaatgcc gccagtgagc
    96181 cgcctgaaac gccaatttca ttgagcaagg agtcaatcag cggtgcctgt gtgcggtaag
    96241 agagagcggc tgataatgct tgttccgcca ggtttccgcc ccccacatta cccgtgtttg
    96301 catcacccgc agcgctgcca cgattcagac catcgacctg aataatcttg ataccgtcaa
    96361 tagatttcat cggctcaacg gatttttcta ttaccgcagg tagcgcctgc aacaaggcca
    96421 gtttgaattt aaggctggtt tgttcatcag aaagtacgtt gatagcatcg ttcagcgcac
    96481 gttgtgcttc tgcttccgcc agaccctttt tacgtgtagc ttcggctaac tcaacgatag
    96541 ccgccgcctg catctctgcg gcttcttttt ctgctttcgc ccgcacggtc agttcaaccg
    96601 ctttggtttc tgcatcctgc gcggcagcga ttagggcaac ttgtttagca cgatcggctt
    96661 ctgcggtctg gcgagtggtt tctacgtttt gttgcgcact tactgcttct gcgagtgcaa
    96721 ggttagcacg cgcttctgcc tgggactgtt gttccgattt ggcggcaata gcgatcgatt
    96781 tcgtctggtt ggcgatttcg gtgacctgct gttgttcgat ctctttaata cgaacttcac
    96841 gttcagcctc aacctttctt gagcggacgg cctgctcgcg ctcgatctcc gtttcctgaa
    96901 tttgtcgttc agccagaatt cgcgtctact ctgcttcacg acgacgttca gcttcaaaag
    96961 ccgcaatacg tgcattctgt tcggcagtac gggttttaac ctgctgctcc tgctcaagcg
    97021 tcataaacgc ttcttgttgt tcaatctcca acttgcgcga aagtgcatca cggtttttct
    97081 cacgcaccgc aacttctaca tcctgttcaa cttcgttacg ttcgcgacga cggcgctccg
    97141 tctcctgagt cagtttggtt aaaccttcgg cgtcaaaggc attgttcgga ttgaaatgtt
    97201 ctttcgaggt ctggttaaag ttggtaagtg aaacgctttc cagctcaaga ccgtttttcg
    97261 acaggtcttc tgcgacagta ttttgtactc cctgcacgaa gttctcgcgg gtatcctgta
    97321 actcatgcat ggtcatttgt gcagctgttg cacggagggc atcgacaaat ttatcttcaa
    97381 ccaacatacg taagtcttct ggtgatagcg tgcgttgccc caacgtctgc gccgcagtgg
    97441 cgatgccttc tacagacggt tttacccgta caaagaaagc gactactaca tcgacacgca
    97501 tacgatcttt cgtaatcagg ctatcaatgg ttgagcggct gacttccagc ttcagagtat
    97561 tcatattgat ggggattatt tcatgaaaga tcggcatcac gattgcgcca ccgctcatca
    97621 ccactttttg cccacctaaa ccagtacgaa caaaagcttg ctctgccgaa gcgcgacgat
    97681 agagcctggc gaaaataatt ccaataataa acagaatgca tacggcaata atcgcggtaa
    97741 acatccagga gggcacagaa ttaacaatat catccattat taaatttcct tttgattaat
    97801 aaattacaat atttgtggcc agggattgtt ttccgccaaa tatcgcgttg cagaaagtcg
    97861 gcaaataatt aacactttgt ctcctttagt gaaaaatttt ccttcttcag gctccagtag
    97921 taaataatgt atttggccaa attgatcggt gagttttcct tcgcagggat tacctgaagt
    97981 ggcttgatga ccggtaatta atgccatgct accaatatat tcttcttctg taatagctga
    98041 actatggtcg cgtggaatcc agggcgcaac tattttaccg gtataatgca ccgcaataat
    98101 cgtaaacagt aaactgacag gaaccacgaa caggttcgac agtggtgatt gccagaccat
    98161 gatgcaggca tgctgcaaca ggatgccaat aagaccaaaa aaaccagcca gcaagcagag
    98221 aacgacgaga gctggcaatc ttccgatatt gagataatga agtgcctggc taatatgacc
    98281 tgtggttatg gaatcataat ggtcaagatg tgcatcaaga gcaccggaga gcatgtgacc
    98341 gcagataagc gctaaaattt ctaaaaggcc gatcaacagt acaaaggaaa tagcgaaaag
    98401 ataaggggta ttatagtcgg cgaataaaat catatgtcac tccaatgtcc atttagtgac
    98461 gcaatattta ctttgggata gtttccttgc ttacatttat ctttgttaat aataacacat
    98521 gttagagaag ttctggaaga gaaagttttt taatatggtt ttttattttt cactaaaaag
    98581 tgtgatcggg gacaatatat ttacgcacgt tatgtttaaa ggcactacac tgattgggga
    98641 atactgaaat cagaaaaagc atatggcctt aaaaggtatg cgataaccgg aggatgtgct
    98701 tatggatcat agtcttaatt ctttaaataa tttcgatttc ctggcgcgta gttttgccag
    98761 aatgcacgca gaaggtcgcc cggtcgatat tcttgccgtt actggtaaca tggatgaaga
    98821 acatagaacc tggttttgcg cacgttatgc ctggtattgt caacagatga tgcaggcaag
    98881 agagctggag ttagagcact gatataacgg gcctgatggc ccgttttagt gtttgtatta
    98941 gatgctagtt ataggtgatt ttgaggatga cgtctttact aaacggtccc tcaacaacag
    99001 tctctgccgg attcgcacta agttctgccc tgaatggtgc ggtgaccgaa ggtgcactag
    99061 acgatgaaaa agaagcaaat tgtgagaagc ggttaaattc cgttgctgtt ccatctttat
    99121 gaaatatctt caatagcagt ccattaccca tatccagatg gctataatca ctcaaaatat
    99181 ccgtagtgaa aaagctccct aaaatattga attgctcagt acaggccgca ccaggatctt
    99241 ttgtcatgac tacactaaac gtctcactaa cgctggtatt tttaattgtt cgggaattta
    99301 ttttaccaaa gtcaacaacg ccatcagctg gttccagtgc gacattaaca aagcagtcaa
    99361 ggacatgaat attatttaat ccacgaatcc tgaaggtcaa atttttgtct acttctatag
    99421 ctcctgcgcc actcatacct ttaaactcta gagcgatata ttcatcaggt aacaccaggt
    99481 ttttattaac tctattggca ttttttgcta atttaatata gagttgtacg cgagctggaa
    99541 aggttactga taattgaggc tcaggacctc cactacagac agcggggttt tttatagtga
    99601 ttattaaagg tacccgctcc ttttatatca atgcattctg gtgctgcgat agcattaggg
    99661 gcagtcgaat cataatcaac tccattgtat gttacgccaa aaatatagga gttctgatta
    99721 taaaagtcct gaccgcctgg taaaaacatc attatattag tccaggctga tgggtattct
    99781 tctccaggtg atggggcagg taatgcatta ttacagaaga cattcataaa atactcactg
    99841 ctttgccaga tcttatcacc ttttttacca ttactgggga tagaaattct tggtacgtaa
    99901 ccaatgacta cggtatttcc cccttcatgt tcagcatagc agtccattgc ccatacaggt
    99961 aatgcactaa aacctataat aaatataatt acaattaata agtagcgcat attatttatc
   100021 tcgtttggct acggaaacat tacaagagtt attaaccgtc gggcagttta tttcataaaa
   100081 acttaatcca ccaaaatcat tgatataacc aacctgaaaa gctgacggaa catttttaaa
   100141 ctgcgtatct gcatggctat aaggggttac cattaaactt tgcgtttgtg gcaacattgt
   100201 gattccgttt gttccgatat aacccagcgt aacgtgataa ggcatcttat tgttaaagcg
   100261 gatcgaacca tcatttctac ggatgatttc aactttttcg taagacatta ccccatcatc
   100321 ttctaattca atagcttttg gtcgccagaa tagtttcaat gcgtgctgca tagtgacttg
   100381 catcacattt tttttatctg tttttggcgg tatttctcgc acgttaaaat agaagagcgt
   100441 ttctctgtca ttaggcagtt gagcaatttt ttcctgactt atcattctta tttgaatttg
   100501 ctcactggga tttaaacgta ccataggggg aaccgtgaca ataaagtcac gtgatttatt
   100561 gcctttcgta tcttctatcc atgtttgcgc cagatagggt agatttttgc tttgattagt
   100621 aagttttaat gcttcccctt tatctgactc attcattatg atacgggtac ggtccgggat
   100681 gatggcaccc caggcaatgc tgctcatgca ggcgagtaat actcctgctg caatgagatt
   100741 ctttgtgttt aagtagcgca ttagtttcat cctgaattaa ttttggctaa tacacggtaa
   100801 aataaccgtt ccggtggtta agttgctgct attgggtggt tgaataaaac aggatttatc
   100861 accccagcgt aaagtaagtt tgctgtcatc ctgaatgccg ttgaggtata caaagccgcc
   100921 atcagcaacc agtcctaatt ctttatggct ggttttatct tttacgctaa tccccagtgg
   100981 tgggtgacta ccatctgcca ggcgaataac ccccatgatt tgatagccct tgcgggtatc
   101041 aagtttggcg tagccaatag caccttcggt taaggtggtg gtgataaccg agttttcgat
   101101 atccacattt tctggaagat ttcgggtatc aacgcttaat gatgttgtta tgtaactgct
   101161 gactgatgga ataactccga tgccataccg attagttacc gcgcgtttat tgttcaacgg
   101221 aatatcttct gcaccatctg cgtcgatcat aaaacgcgag tcagcactac cactatagtc
   101281 atggaaagcg gctccgtgac gagtcgccgt gaatgaacca ttccaactgg cattcagaga
   101341 ataataactg tctcgctgtg atgtcccgga gagatagaga cgaccgttct cggtattatg
   101401 ctgataactg gcgcgtagtg acgctttcat atctttgaat tcatcattgt cgcctgaagc
   101461 agaaacgctc caggaattat ttcgatcgga agagtcatac cacgaagcag tatgcgaaat
   101521 cttgttatcc tgatttcgtt gcataccata gctcaatgtt ctactggttc cccagggaat
   101581 cgaaatattt aggtacagtt gatcctggtt gtcttcttcc cagtttattc ggctaaatgt
   101641 tagagaagtg gagacgtttt ttaatggccc gatatcaaaa tcgcggctaa gtgataatga
   101701 ataattcaca ttactgctgg cgtcccagta agtattacga gccaaactta cgtatgtatt
   101761 taaccttaat gattcaagat actggttaaa cgtgacaata taactctctt tttcattctg
   101821 ataattcgtg taatggttag tgtcattgat atattcattc attgtcacaa agtttttatc
   101881 agagaaacgg taaccagcga aagtgagctg gctaccggta gattcaaaac gttttgaata
   101941 gttagcgcgg tagctataac ccgtttcttt atcctgatta tgcaactgag catcagatcg
   102001 tgtgacatca aaagataatg aacccaatga gttaaggtta aagccaacgc cggcagccag
   102061 agattgataa tcacggtttg ttaaaactga accaccatac agggatacat tgttcagcca
   102121 accccatgag aattcaccag tccagaagaa gggttgttgt aaggaatcac caccaacgct
   102181 ggtcggttta cccatagcag tggtgtagcg aatctgccct ttacgcgtca gataaggaat
   102241 actattagat gcaacttgcc attgcgtgac ttgcccgtcc tcttcttcaa ccttgacgtc
   102301 cagttgcccc tgtaatgtat tgaataaatt agtaatagca aatgggccag gaggaacatt
   102361 ttcttgataa ataatacggt tgttctgtga gacagtgacc tttgcattcg tttgtgcaac
   102421 acccgtgatt tgcggtgcgt aaccacgtag atcaggcggc aacatattct catcgctttt
   102481 caacgaaacg ccagaaaaat ggaaagagtc gaaaatagag gattcagtat cgtattgacc
   102541 tatagttagt tttgcattaa ttgctgggat tgggcgaaac aaataaatac gcggaaaatc
   102601 caggttgttc gcctggcttt tgccattgtt aaatgactga tcgtactggt aatcagtacg
   102661 caggcgccag gccccaagat taaccccagc ctgcccatag gaacttatat tttgtgaatt
   102721 atcgccctga tgtggtgcat aatgagaggc atataaattg tagtcaagaa atgccccagc
   102781 aataccatga tcccaaagtt caggaggcgt ccagtttgca tctttgtatt ttaaccatgc
   102841 ctgaggtgca gatatggata attgcatttt ccctttatcg agataagttg taatttgttt
   102901 ttctttttcg atagggtaac aaccatcgat caggttttgc tgagcctctt caataatctc
   102961 atcagtaaag cctaataatg ttaattgctc tggagtcaga caaactgcag tttggttatc
   103021 aattaatacc cattgcaggt ccagatggat tggcgtcctt ttattattaa taagagcgtt
   103081 taatgagtat ttacctacgg gtaattggcc atcagtttca aattgggaaa ggttaacgtt
   103141 ttcacgatct tcggcttcga ttaaatcttt gttgaattca acggcataag ctgacgcaca
   103201 accaatgatg agaacggata atgaacaata aatattttta atcaattcgc ttattgtggt
   103261 taattttaat tttttataca tctgttccac ttatcattaa tccgttaatg atgtggccgg
   103321 gcgaacccgg cctaatgtga ttactcgtaa gcaatcttat agtcagcctg agcgtaaacg
   103381 ttgcccgggg tagcatcttt gcttggggat tccatacgag ctttgaagtt cagttcctga
   103441 tagctgttag tattgttgaa tgtaattact accggagtac ctaaagtcac ttcgccgctc
   103501 tggctatcca gaatacgtac gccaacgcct gtggcaccac cgatttcagt ttctttggtg
   103561 tttttcagca ggtcagtctg acccgtcata gtattcgctg tggtgaaagt aaccttcgct
   103621 tttgaataag tggttccacc ctggctcaac atgcagtctt gcaggtgaat agtgaaaggc
   103681 tcagccaaag atttctggtc gcttttcaga acggtatctg ctacttcacc cagatttaca
   103741 gttaagtctt cgtcacctgg tttaattgaa catggagcag agataacttc acctttaaat
   103801 ttaacaatgc cgtgaccttc gtcagcaaat acgttattgg aaagaatcat tgctacgcca
   103861 gcaacaagaa gtgacttttt aaaagcagac ataatattcc tttatataaa tataagtaga
   103921 aaatgaat
//
LOCUS       DAZFKM010000002        90907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFKM010000002 DAZFKM010000000
VERSION     DAZFKM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKAY_000116"
     CDS             complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHKAY_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO5698863.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKAY_000117"
     CDS             complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHKAY_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO5698864.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKAY_000118"
     CDS             4356..5549
                     /gene="acrA"
                     /locus_tag="ACHKAY_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO5698865.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKAY_000119"
     CDS             5572..8721
                     /gene="acrB"
                     /locus_tag="ACHKAY_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO5698866.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            9267..9641
                     /gene="tomB"
                     /locus_tag="ACHKAY_000120"
     CDS             9267..9641
                     /gene="tomB"
                     /locus_tag="ACHKAY_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO5698867.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            9667..9885
                     /gene="hha"
                     /locus_tag="ACHKAY_000121"
     CDS             9667..9885
                     /gene="hha"
                     /locus_tag="ACHKAY_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO5698868.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            10057..10608
                     /gene="maa"
                     /locus_tag="ACHKAY_000122"
     CDS             10057..10608
                     /gene="maa"
                     /locus_tag="ACHKAY_000122"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO5698869.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHKAY_000123"
     CDS             10724..11194
                     /gene="ylaC"
                     /locus_tag="ACHKAY_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO5698870.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHKAY_000124"
     CDS             11358..12908
                     /gene="pdeB"
                     /locus_tag="ACHKAY_000124"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO5698871.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHKAY_000125"
     CDS             complement(12950..13303)
                     /gene="ybaA"
                     /locus_tag="ACHKAY_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO5698872.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13423..13519)
                     /gene="ffs"
                     /locus_tag="ACHKAY_000126"
     ncRNA           complement(13423..13519)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKAY_000126"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13681..13992
                     /locus_tag="ACHKAY_000127"
     CDS             13681..13992
                     /locus_tag="ACHKAY_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO5698873.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHKAY_000128"
     CDS             complement(14023..14595)
                     /gene="ybaY"
                     /locus_tag="ACHKAY_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO5698874.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            14813..15673
                     /gene="tesB"
                     /locus_tag="ACHKAY_000129"
     CDS             14813..15673
                     /gene="tesB"
                     /locus_tag="ACHKAY_000129"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO5698875.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHKAY_000130"
     CDS             complement(15722..17008)
                     /gene="amtB"
                     /locus_tag="ACHKAY_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO5698876.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHKAY_000131"
     CDS             complement(17038..17376)
                     /gene="glnK"
                     /locus_tag="ACHKAY_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO5698877.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHKAY_000132"
     CDS             complement(17557..19338)
                     /gene="mdlB"
                     /locus_tag="ACHKAY_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5698878.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHKAY_000133"
     CDS             complement(19331..21103)
                     /gene="mdlA"
                     /locus_tag="ACHKAY_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO5698879.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHKAY_000134"
     CDS             complement(21133..21591)
                     /gene="decR"
                     /locus_tag="ACHKAY_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO5698880.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHKAY_000135"
     CDS             complement(21744..22562)
                     /gene="cof"
                     /locus_tag="ACHKAY_000135"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO5698881.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHKAY_000136"
     CDS             22662..24362
                     /gene="ybaE"
                     /locus_tag="ACHKAY_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO5698882.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            24427..25122
                     /gene="queC"
                     /locus_tag="ACHKAY_000137"
     CDS             24427..25122
                     /gene="queC"
                     /locus_tag="ACHKAY_000137"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO5698883.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHKAY_000138"
     CDS             complement(25174..25572)
                     /gene="fadM"
                     /locus_tag="ACHKAY_000138"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO5698884.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHKAY_000139"
     CDS             complement(25666..26037)
                     /gene="ybaV"
                     /locus_tag="ACHKAY_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO5698885.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHKAY_000140"
     CDS             complement(26188..28059)
                     /gene="ppiD"
                     /locus_tag="ACHKAY_000140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5698886.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHKAY_000141"
     CDS             complement(28251..28541)
                     /gene="hupB"
                     /locus_tag="ACHKAY_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO5698887.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHKAY_000142"
     CDS             complement(28732..31086)
                     /gene="lon"
                     /locus_tag="ACHKAY_000142"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO5698888.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHKAY_000143"
     CDS             complement(31274..32548)
                     /gene="clpX"
                     /locus_tag="ACHKAY_000143"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO5698889.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHKAY_000144"
     CDS             complement(32674..33297)
                     /gene="clpP"
                     /locus_tag="ACHKAY_000144"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO5698890.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHKAY_000145"
     CDS             complement(33544..34842)
                     /gene="tig"
                     /locus_tag="ACHKAY_000145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5698891.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHKAY_000146"
     CDS             complement(35186..35503)
                     /gene="bolA"
                     /locus_tag="ACHKAY_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO5698892.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            35808..36386
                     /gene="yajG"
                     /locus_tag="ACHKAY_000147"
     CDS             35808..36386
                     /gene="yajG"
                     /locus_tag="ACHKAY_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5698893.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            36430..37905
                     /gene="ampG"
                     /locus_tag="ACHKAY_000148"
     CDS             36430..37905
                     /gene="ampG"
                     /locus_tag="ACHKAY_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO5698894.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHKAY_000149"
     CDS             38365..39312
                     /gene="cyoA"
                     /locus_tag="ACHKAY_000149"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO5698895.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHKAY_000150"
     CDS             39334..41325
                     /gene="cyoB"
                     /locus_tag="ACHKAY_000150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO5698896.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHKAY_000151"
     CDS             41315..41929
                     /gene="cyoC"
                     /locus_tag="ACHKAY_000151"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO5698897.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHKAY_000152"
     CDS             41929..42258
                     /gene="cyoD"
                     /locus_tag="ACHKAY_000152"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO5698898.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHKAY_000153"
     CDS             42270..43160
                     /gene="cyoE"
                     /locus_tag="ACHKAY_000153"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO5698899.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            43345..43656
                     /locus_tag="ACHKAY_000154"
     CDS             43345..43656
                     /locus_tag="ACHKAY_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO5698900.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            43640..44182
                     /locus_tag="ACHKAY_000155"
     CDS             43640..44182
                     /locus_tag="ACHKAY_000155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5698901.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(44238..45173)
                     /locus_tag="ACHKAY_000156"
     CDS             complement(44238..45173)
                     /locus_tag="ACHKAY_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5698902.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            45581..46945
                     /gene="yajR"
                     /locus_tag="ACHKAY_000157"
     CDS             45581..46945
                     /gene="yajR"
                     /locus_tag="ACHKAY_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5698903.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(47073..47564)
                     /gene="yajQ"
                     /locus_tag="ACHKAY_000158"
     CDS             complement(47073..47564)
                     /gene="yajQ"
                     /locus_tag="ACHKAY_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO5698904.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            47732..48643
                     /gene="panE"
                     /locus_tag="ACHKAY_000159"
     CDS             47732..48643
                     /gene="panE"
                     /locus_tag="ACHKAY_000159"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO5698905.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            48606..49196
                     /gene="yajL"
                     /locus_tag="ACHKAY_000160"
     CDS             48606..49196
                     /gene="yajL"
                     /locus_tag="ACHKAY_000160"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO5698906.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(49250..50698)
                     /gene="thiI"
                     /locus_tag="ACHKAY_000161"
     CDS             complement(49250..50698)
                     /gene="thiI"
                     /locus_tag="ACHKAY_000161"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO5698907.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            50904..51146
                     /gene="xseB"
                     /locus_tag="ACHKAY_000162"
     CDS             50904..51146
                     /gene="xseB"
                     /locus_tag="ACHKAY_000162"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO5698908.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            51146..52045
                     /gene="ispA"
                     /locus_tag="ACHKAY_000163"
     CDS             51146..52045
                     /gene="ispA"
                     /locus_tag="ACHKAY_000163"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO5698909.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            52070..53932
                     /gene="dxs"
                     /locus_tag="ACHKAY_000164"
     CDS             52070..53932
                     /gene="dxs"
                     /locus_tag="ACHKAY_000164"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO5698910.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            54112..55086
                     /gene="yajO"
                     /locus_tag="ACHKAY_000165"
     CDS             54112..55086
                     /gene="yajO"
                     /locus_tag="ACHKAY_000165"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO5698911.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(55140..55658)
                     /gene="pgpA"
                     /locus_tag="ACHKAY_000166"
     CDS             complement(55140..55658)
                     /gene="pgpA"
                     /locus_tag="ACHKAY_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO5698912.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(55636..56613)
                     /gene="thiL"
                     /locus_tag="ACHKAY_000167"
     CDS             complement(55636..56613)
                     /gene="thiL"
                     /locus_tag="ACHKAY_000167"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO5698913.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(56691..57110)
                     /gene="nusB"
                     /locus_tag="ACHKAY_000168"
     CDS             complement(56691..57110)
                     /gene="nusB"
                     /locus_tag="ACHKAY_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO5698914.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(57130..57600)
                     /gene="ribE"
                     /locus_tag="ACHKAY_000169"
     CDS             complement(57130..57600)
                     /gene="ribE"
                     /locus_tag="ACHKAY_000169"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO5698915.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(57689..58792)
                     /gene="ribD"
                     /locus_tag="ACHKAY_000170"
     CDS             complement(57689..58792)
                     /gene="ribD"
                     /locus_tag="ACHKAY_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO5698916.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(58796..59245)
                     /gene="nrdR"
                     /locus_tag="ACHKAY_000171"
     CDS             complement(58796..59245)
                     /gene="nrdR"
                     /locus_tag="ACHKAY_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO5698917.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            59396..59935
                     /gene="yajI"
                     /locus_tag="ACHKAY_000172"
     CDS             59396..59935
                     /gene="yajI"
                     /locus_tag="ACHKAY_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5698918.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            60234..61118
                     /gene="tsx"
                     /locus_tag="ACHKAY_000173"
     CDS             60234..61118
                     /gene="tsx"
                     /locus_tag="ACHKAY_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO5698919.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(61295..61642)
                     /gene="yajD"
                     /locus_tag="ACHKAY_000174"
     CDS             complement(61295..61642)
                     /gene="yajD"
                     /locus_tag="ACHKAY_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO5698920.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(61771..62742)
                     /gene="secF"
                     /locus_tag="ACHKAY_000175"
     CDS             complement(61771..62742)
                     /gene="secF"
                     /locus_tag="ACHKAY_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO5698921.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(62753..64600)
                     /gene="secD"
                     /locus_tag="ACHKAY_000176"
     CDS             complement(62753..64600)
                     /gene="secD"
                     /locus_tag="ACHKAY_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO5698922.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(64628..64960)
                     /gene="yajC"
                     /locus_tag="ACHKAY_000177"
     CDS             complement(64628..64960)
                     /gene="yajC"
                     /locus_tag="ACHKAY_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO5698923.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(64983..66110)
                     /gene="tgt"
                     /locus_tag="ACHKAY_000178"
     CDS             complement(64983..66110)
                     /gene="tgt"
                     /locus_tag="ACHKAY_000178"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO5698924.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(66166..67236)
                     /gene="queA"
                     /locus_tag="ACHKAY_000179"
     CDS             complement(66166..67236)
                     /gene="queA"
                     /locus_tag="ACHKAY_000179"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO5698925.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            67329..67910
                     /gene="acpH"
                     /locus_tag="ACHKAY_000180"
     CDS             67329..67910
                     /gene="acpH"
                     /locus_tag="ACHKAY_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO5698926.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(67915..69732)
                     /gene="malZ"
                     /locus_tag="ACHKAY_000181"
     CDS             complement(67915..69732)
                     /gene="malZ"
                     /locus_tag="ACHKAY_000181"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO5698927.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(69888..71260)
                     /gene="proY"
                     /locus_tag="ACHKAY_000182"
                     /pseudo
     CDS             complement(69888..71260)
                     /gene="proY"
                     /locus_tag="ACHKAY_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
     gene            complement(71336..72655)
                     /gene="brnQ"
                     /locus_tag="ACHKAY_000183"
     CDS             complement(71336..72655)
                     /gene="brnQ"
                     /locus_tag="ACHKAY_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO5698928.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(73062..74357)
                     /gene="phoR"
                     /locus_tag="ACHKAY_000184"
     CDS             complement(73062..74357)
                     /gene="phoR"
                     /locus_tag="ACHKAY_000184"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO5698929.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(74415..75104)
                     /gene="phoB"
                     /locus_tag="ACHKAY_000185"
     CDS             complement(74415..75104)
                     /gene="phoB"
                     /locus_tag="ACHKAY_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO5698930.1"
                     /translation="MTRRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            75294..76496
                     /gene="sbcD"
                     /locus_tag="ACHKAY_000186"
     CDS             75294..76496
                     /gene="sbcD"
                     /locus_tag="ACHKAY_000186"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO5698931.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            76493..79636
                     /gene="sbcC"
                     /locus_tag="ACHKAY_000187"
     CDS             76493..79636
                     /gene="sbcC"
                     /locus_tag="ACHKAY_000187"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO5698932.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            79762..80946
                     /gene="araJ"
                     /locus_tag="ACHKAY_000188"
     CDS             79762..80946
                     /gene="araJ"
                     /locus_tag="ACHKAY_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO5698933.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(81089..81997)
                     /gene="mak"
                     /locus_tag="ACHKAY_000189"
     CDS             complement(81089..81997)
                     /gene="mak"
                     /locus_tag="ACHKAY_000189"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO5698934.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            82122..83033
                     /gene="rdgC"
                     /locus_tag="ACHKAY_000190"
     CDS             82122..83033
                     /gene="rdgC"
                     /locus_tag="ACHKAY_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5698935.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(83111..83194)
                     /locus_tag="ACHKAY_000191"
     CDS             complement(83111..83194)
                     /locus_tag="ACHKAY_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698936.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(83191..83532)
                     /locus_tag="ACHKAY_000192"
                     /pseudo
     CDS             complement(83191..83532)
                     /locus_tag="ACHKAY_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(83680..83964)
                     /gene="ppnP"
                     /locus_tag="ACHKAY_000193"
     CDS             complement(83680..83964)
                     /gene="ppnP"
                     /locus_tag="ACHKAY_000193"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO5698937.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(84036..84713)
                     /gene="aroM"
                     /locus_tag="ACHKAY_000194"
     CDS             complement(84036..84713)
                     /gene="aroM"
                     /locus_tag="ACHKAY_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO5698938.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(84971..85162)
                     /gene="yaiA"
                     /locus_tag="ACHKAY_000195"
     CDS             complement(84971..85162)
                     /gene="yaiA"
                     /locus_tag="ACHKAY_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO5698939.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(85212..85736)
                     /gene="aroL"
                     /locus_tag="ACHKAY_000196"
     CDS             complement(85212..85736)
                     /gene="aroL"
                     /locus_tag="ACHKAY_000196"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO5698940.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(85919..86377)
                     /gene="yaiI"
                     /locus_tag="ACHKAY_000197"
     CDS             complement(85919..86377)
                     /gene="yaiI"
                     /locus_tag="ACHKAY_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO5698941.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            86497..87306
                     /gene="proC"
                     /locus_tag="ACHKAY_000198"
     CDS             86497..87306
                     /gene="proC"
                     /locus_tag="ACHKAY_000198"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO5698942.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(87323..88438)
                     /gene="adrA"
                     /locus_tag="ACHKAY_000199"
                     /gene_synonym="dgcC"
     CDS             complement(87323..88438)
                     /gene="adrA"
                     /locus_tag="ACHKAY_000199"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO5698943.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(88540..88860)
                     /gene="psiF"
                     /locus_tag="ACHKAY_000200"
     CDS             complement(88540..88860)
                     /gene="psiF"
                     /locus_tag="ACHKAY_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO5698944.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(88979..90394)
                     /gene="phoA"
                     /locus_tag="ACHKAY_000201"
     CDS             complement(88979..90394)
                     /gene="phoA"
                     /locus_tag="ACHKAY_000201"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO5698945.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(90495..90755)
                     /gene="iraP"
                     /locus_tag="ACHKAY_000202"
     CDS             complement(90495..90755)
                     /gene="iraP"
                     /locus_tag="ACHKAY_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO5698946.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        22065 a        24122 c        22566 g        22154 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
       61 tcgccccctt tggttattac cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt
      121 tgacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
      181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
      241 tacgactacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
      301 caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
      361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
      421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
      481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
      541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
      601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
      661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
      721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
      781 tagtaatggc atacgacgcg ccctgacgca tattcagtcg cgagagcacc agcacttcca
      841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
      901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
      961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
     1021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
     1081 taacctgttc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
     1141 tcaccagatt ctgccgacgc gccaacgcac gacgccaggc gatacgccgc gccgctacgc
     1201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
     1261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
     1321 tcaatgcaat cgggataatc gacagcacgg taatggtgac cagtcgcatg gtgtgcgact
     1381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
     1441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
     1501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
     1561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
     1621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
     1681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
     1741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
     1801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
     1861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
     1921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
     1981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
     2041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
     2101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
     2161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
     2221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
     2281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
     2341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
     2401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
     2461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
     2521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
     2581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
     2641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
     2701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
     2761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
     2821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
     2881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
     2941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
     3001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
     3061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
     3121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgtcgtagc tgaactgtct
     3181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
     3241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
     3301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
     3361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
     3421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
     3481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
     3541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
     3601 cacaggagat acatctccag taagatggca acgtaatcgc gggcttcttt tttaagatca
     3661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
     3721 attgctgcgc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
     3781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
     3841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgc
     3901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
     3961 ggatcgccag ggaattttgc ctgatactca agctctagtt caccaatatt ggattctgac
     4021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
     4081 cgcgtaacgc cagctgcttt tgcaatctca cccagcgagg tggatgatac cccctgctgt
     4141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     4201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     4261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
     4321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
     4381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
     4441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
     4501 ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc gcagaagttc
     4561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
     4621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
     4681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
     4741 atcagaaact gctcggtact cagtacatca gtaagcaaga gtacgatcag gctctggctg
     4801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
     4861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
     4921 tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc gtgcagcaac
     4981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg
     5041 aactggcgaa tggcacgctg aaacaagaga acggcaaagc caaagtgtcg ctgatcacca
     5101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
     5161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
     5221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
     5281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
     5341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
     5401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
     5461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
     5521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
     5581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
     5641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
     5701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
     5761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
     5821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
     5881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
     5941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
     6001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
     6061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
     6121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
     6181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
     6241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
     6301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
     6361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
     6421 caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
     6481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
     6541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
     6601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
     6661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
     6721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
     6781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
     6841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
     6901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
     6961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
     7021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
     7081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt cgagaagagc
     7141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctggtg
     7201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
     7261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
     7321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
     7381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
     7441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
     7501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
     7561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
     7621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt
     7681 gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg tctggaagat
     7741 accccgcagt ttaagattga tatcgaccgg gaaaaagcgc aggcgctggg tgtttctatc
     7801 aatgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
     7861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
     7921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc gttctcggca
     7981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa tggcctgcca
     8041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
     8101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
     8161 tatcaggaac gcctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
     8221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ttccgttctc cgttatgctg
     8281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
     8341 gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa gaacgcgata
     8401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
     8461 acgcttgatg cagtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
     8521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
     8581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
     8641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
     8701 catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa gcggcctttt
     8761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
     8821 aaaccagtat taaccacgga taaaattcat aaaaaatact gattgttagt taatttatat
     8881 taagtagcgc taatagattt aataatccat aatcatttag aggctattct taattatttg
     8941 cggtaattct ttattcattc ctcggttatt acgtcatatt cagagcaatc ctggtattag
     9001 tgtcaccaat ttcatctggc gataatcctg aaatgttatg aacagttcga gcaaactgct
     9061 tttacctgct gcgggttagt gctaatatga aaaagtgagt cctgtcccga ttccttccta
     9121 attgtaattt ttcgtaataa tgcgatgaaa acctgcaaag agtggcttat agttaagcta
     9181 acaaacgaga gggcaagtcc aggtcagtaa gttttttcca tcccgaaagg tgtccgttag
     9241 ttcaaccact aagaagggga cgcgttatgg atgaatactc acccaaaaga catgatatcg
     9301 cacagcttaa gtttctctgt gaaaccctgt atcatgactg tcttgcaaac cttgaagaaa
     9361 gcaatcatgg ctgggttaac gacccaacct cggcgatcaa cctccagttg aatgaactga
     9421 ttgagcatat tgcgaccttc gcacttaatt acaaaattaa gtataatgaa gacaataagc
     9481 tcattgagca gatcgacgaa tatctggatg acacctttat gttgttcagc agttatggta
     9541 ttaatatgca ggatcttcag aaatggcgga agtcaggtaa tcgactattc cgttgttttg
     9601 tcaatgcgac gaaagagaat cctgcgagtt tatcttgtta gaattattac aaccatgggt
     9661 agaagtatgt ccgaaaaacc tttaacgaaa accgattatt taatgcgttt acgtcgttgc
     9721 cagacaattg acacgctgga gcgtgttatc gagaaaaata aatacgaatt atcagataat
     9781 gaactggcgg tattttactc agccgcagat caccgcctcg ccgaattgac catgaataaa
     9841 ctatacgaca agatcccttc ctcagtatgg aaatttattc gctaataaat aattcgcttt
     9901 cggagctata accggctgtt tattaagaat tttatacttt ttcaccatga agacataccc
     9961 tatgtgatct ttatcacaca gatgtaatag gaacgttctc ttcactgact tttcgtctta
    10021 ctgtgttgcc gcattttcag caaccggagg tcagtaatga gcacagaaaa agaaaagatg
    10081 attgctggtg agttgtatcg ctcggcagat gagacgttat ctcgcgatcg cctgcgcgct
    10141 cgtcagctta ttcaccgata caatcattcc ctggcggaag agcacacatt acgccagcaa
    10201 attctcgctg atctattcgg tcaggtgaca gaggcttata ttgagccaac gtttcgctgt
    10261 gactatggct ataacatttt tctcggtaat aattttttcg ccaacttcga ttgcgtgatg
    10321 cttgatgtct gccctattcg catcggtgat aactgtatgt tggcaccagg cgttcatatc
    10381 tacacggcaa cacatcccat cgaccctgta gcacgtaata gcggtgctga actggggaaa
    10441 cccgtcacca tcggtaataa cgtctggatt ggcggacgcg cggtcattaa ccctggtgtg
    10501 accattggtg ataacgtcgt ggtagcctca ggtgcagttg tcacaaaaga tgtcccggac
    10561 aacgttgtcg tgggcggtaa tccagccaga ataattaaaa aattgtaatc ggtttttcgc
    10621 aactgttatg caaaattgtg gtagatctgt tacttcccct ctactattcc cacgttaaaa
    10681 tagggtgttc cctggaaagt tgcagatacc acgaaggcaa acgatgaccg aaatacaacg
    10741 cctgctgacc gaaacgattg agtctctgaa tacccgcgaa aaacgcgaca acaaaccccg
    10801 ctttagtatc agttttatcc gtaaacatcc ggggctgttt atcggtatgt acgttgcttt
    10861 ttttgccacc ctggcggtga tgttgcagtc cgaaacgctg tcaggctctg tctggctact
    10921 ggttgtatta tttatcctgc ttaatggttt cttctttttc gatgtctacc cacgctaccg
    10981 ctatgaagat atcgacgtgc tggatttccg cgtttgctat aacggcgaat ggtacaacac
    11041 gcgctttgta cctgccgcgc tggttgaagc catcttgaac tctccgcgtg tcgcggatgt
    11101 tcataaggaa caactgcaaa aaatgatcgt ccgtaaaggt gaactgtctt tttacgatat
    11161 ttttaccctc gctcgcgccg aatcaacatc ttaagttagg gttacatacc aggcgtaaag
    11221 ctctgcgcct ggtcaaatga caatgatcgt ttccacccat cacttcatga aataccagct
    11281 ctacctcctt atctcctgcc agcctttttc cacaatcaga tatactttcc ctacactgtg
    11341 ttaataagga tatgctggtg agaacacgac atctggtcgg ccttatttcg ggagtactga
    11401 ttctttcagt attgctgcct gtcggcttaa gcatctggct ggcccatcag caggtagaaa
    11461 catcgtttat tgaagagctg gatacctatt cctcccgcgt cgctattcgg gccaataagg
    11521 tggcgacaca agggaaagat gcgctgcaag agctggaaag atggcaaggc gctgcctgta
    11581 gcgaagccca tctcatggaa atgcgtcggg tatcttacag ttatcgctat attcaggaag
    11641 tggtttatat cgataacaac gttccccagt gttcgtctct ggagcatgaa agtccgcccg
    11701 ataccttccc cgagccaggt aaaatttcga aagatggtta tcgtgtctgg ttaacatcgc
    11761 ataacgattt aggcattatc cgttacatgg tcgccatggg aacggcacat tatgtcgtca
    11821 tgatcgaccc cgcttccttt attgatgtca ttccctatag ctcatggcaa attgatgccg
    11881 ccattattgg caatgcccat aacgttgtca taaccagcag cgatgaaatt gctcagggaa
    11941 ttattaccag gctacaaaaa acacccggtg agcatatcga aaataatgga atcatttacg
    12001 atatcctgcc cttcccggag atgaatattt cgatcatcac ctgggcttca acgaaaatgt
    12061 tgcagaaagg ctggcatcgg caagtcttta tttggttacc gctcgggttg gtgattggcc
    12121 agctggcagc gatgtttgtg ctgcgtattt tgcgccgtat ccagtcaccg catcatcggc
    12181 tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta tcagccgatt gtctccttag
    12241 ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg gccgcagaca gacggtagtt
    12301 ggttgtcacc agatagtttt attccgctgg cacagcaaac gggcctttct gagccattga
    12361 cgctactgat tataagaagc gtctttgaag atatgggcga ctggctgcgt cagcatccac
    12421 agcagcatat ttcgatcaat cttgaatcca ccgtgctcac ctcggaaaaa atcccgcaat
    12481 tgctgcgtga aatgatcaat cactatcagg ttaatcccag acagatcgcg cttgaactca
    12541 ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat aatttctcgc taccgggagg
    12601 cgggccatga aatttatctt gatgattttg gtacgggata ttcaagttta agttatttac
    12661 aggatctgga tgtcgacatt ctgaagatcg ataaatcttt cgttgatgcg ctggaatata
    12721 aaaatgtcac gccgcatatc atcgaaatgg caaaaacact gaaactgaaa atggtagcgg
    12781 agggaatcga aaccagtaaa caagaagagt ggctacgcca gcatggcgtg cactacggtc
    12841 agggctggct ctacagcaag gcattaccga aagaagattt cttacgctgg gccgagcaac
    12901 atttgtgaat ttttgggctg acggcacgcc gcacagcccg tcacgctacc tattcgtcga
    12961 tgattgactc gaatccgcca tagatcattc gcttgccgtc aaacggcatg gactcgccga
    13021 actctttcat ccgtgggtcc gacatcatct tttgattagc agcgtcgcgg acctcttttg
    13081 aagggtattc aatccagcta aagacaacct cttcattctc ttccgctttc accgccatac
    13141 gaaaatcggt cactttgcca tccggtacat cgctggccca gcattcgaca atacgaagcg
    13201 cgccaaactc tttaaacaat ggcgcagcct tagcggccat ttcccgatag gcatcctttt
    13261 tatcggcagg aacggcaacc acaaaaccat caacatactt catcagacat acctccgaag
    13321 cgtactgcgc agccagacaa ttcttgcgac gtcagttgtg gttaaagttt agccatgttg
    13381 gttttttttg acgaaacggt gggaggcaga aatgggtggg ggccctgcca gctacatccc
    13441 ggcacacgcg tcatctgcct tggctgcttc cttccggacc tgacctggta aacagagtag
    13501 cgttgcggga gaaccaacag gcccccattg agagcgttga gaaccaacgc gcaggcgcat
    13561 tatcactgct gccccggcta attgcaaccc atcgcgtgcc agatgctggt ttcttgcgca
    13621 atgcgacttc actcgggcgt ttttccagat tatgctgaaa aattacctca ggaagaaaag
    13681 atggaaaaag aagattcatt tccccaacgc gtctggcaaa tcgtcgccgc tattcccgaa
    13741 ggctatgtca ccacttacgg tgatgtggcg aaactggcgg gatcgccccg cgccgcgcgc
    13801 caggtgggcg gtgtgttaaa gcgtctccct gaaggcagca ccttaccctg gcaccgggtg
    13861 gttaatcgcc acggcacaat ttcgctaacc ggaccggatt tacagcgtca gcgacaggca
    13921 ttactggcag aaggtgtgat ggtatcggga agcgggcaaa tcgacttgca gcgttatcgc
    13981 tggaactact aaccctctcc gggcggagag ggttaacgtc gcttaatatt gcgtaggagc
    14041 tggaactgcc gaagacggat tcacctgagt tggtgaagtc gaaggtacgg tagtcgttgc
    14101 accaccgctg gcctgaaccg gcacggcggt ttgctgcacc ggcaccaatg tcaggtcggc
    14161 tttagttccg cccttgttga tcaccggctg aacggtatcg gtaataaata ccagtttgtc
    14221 attcacggta atcgccgcac tcaacagaat acgcgcgttc ggctgaacat ctgccggatt
    14281 aaatggcaga acaaagctga atggtgactg tttaccttcg gtacgcaccg ctttctgcgc
    14341 cagcactttt gacggtgcat cggctaacga cgcgtcagaa agtgtcacgg tcagcacagc
    14401 atcaggcggc agtgcgactt tctgccggat ccagacggta ccggagacat tcggttgctg
    14461 gatagcaggt tgttgtgttg ctgaaataga tgtatttgca gccggggctg gcgtctgaat
    14521 atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca accgctaaac cactggccat
    14581 gtgcacgagt ttcattcatt tctccttatt atcaatgcac cagcgggcta actttcctcg
    14641 ccggaagagt ggttaacaaa atagtaacgt caacaagtgt ggcacacatc acgcatttct
    14701 gcctgtaatt agcccgtaat tcagaccatt gcacccatcg gaccagtagc aaatttgcgt
    14761 tatactcaac tcactttggc ttgctgcggc agctttgtta ctggagagtt atatgagtca
    14821 ggcgctaaaa aatttactga cattgttaaa tctggaaaaa attgaggaag gactctttcg
    14881 cggccagagt gaagatttag gtttacgcca ggtgtttggc ggccaggtcg tgggtcaggc
    14941 cttgtatgct gcaaaagaga ccgtccctga agagcggctg gtacattcgt ttcacagcta
    15001 ctttcttcgc cctggcgata gtaagaagcc gattatttat gatgtcgaaa cgctgcgtga
    15061 cggtaacagc ttcagcgccc gccgggttgc tgctattcaa aacggcaaac cgatttttta
    15121 tatgacggcc tctttccagg caccagaagc gggtttcgaa catcaaaaaa caatgccgtc
    15181 cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc gcccaatcgc tggcgcacct
    15241 gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt ccgctggaag tccgtccggt
    15301 ggagtttcat aacccactga aaggtcacgt cgcagaacca catcgtcagg tgtggatccg
    15361 cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag tatctgctcg gttacgcttc
    15421 tgatcttaac ttcctgccgg tagctctaca gccgcacggc atcggttttc tcgaaccggg
    15481 gattcagatt gccaccattg accattccat gtggttccat cgcccgttta atttgaatga
    15541 atggctgctg tatagcgtgg agagcacctc ggcgtccagc gcacgcggct ttgtgcgcgg
    15601 tgagttttat acccaggacg gcgtgctggt tgcctcgacc gttcaggaag gggtgatgcg
    15661 taatcacaat taaaaaaaat agccggaggt aaaaccgtcc ggctattttt tgcagtgctt
    15721 gttacgcgtt ataggcattc tcgccgtgac tgttgacatc cagcccttct cgctcctgct
    15781 cttccggtac acgcagacca accgtcagat ccgccagttt gtagccaata aatgccacca
    15841 caccggacca gacgatcgta atggcgatgc tttccagctg taccagcaac tgatggccca
    15901 tcgtcacacc ttcagcgaag cccacgccac ccagcgaact ggcggcaaaa atcccagtca
    15961 tgatacagcc gacaatgcca caaacgccgt gcacaccgaa gacatcgcag ggatcatcca
    16021 cccgcagcaa gcgtttgagc atagtaacgc cccacaagcc cgccagacca gctaccacgc
    16081 cgataatcaa cgcgccgcca accccaatgt agccacacgc tggcgtcacg ccgaccagac
    16141 cggcaatcgc gccagaacac gctcccagca gagaaggctt accacgcagc gcccattcac
    16201 cgaagatcca gccaagaatt gccgccgccg ttgcgaccac agtattcaca aatgccagtg
    16261 ccgcgatttc attcgccgtg cccgctgacc cggcgttaaa gccaaaccaa ccaatataga
    16321 gaatggcagt accggtgaag accattggca ggttgtgcgg tttaaacgcc tctttgccga
    16381 agcccacgcg tttgcctatc agatacgcgc ccaccagacc ggcgattgcg gcgttaatat
    16441 gcaccaccgt gccacccgcg aaatccagcg caccgtgaga agccagcaaa ccaccgcccc
    16501 acaccatatg cgcaatcgga atgtaagaga gcgtcagcca taccaccacg aaaatcaaca
    16561 cagctgagaa gcggattcgt tccgccagcg ccccaacgat caagccgacg gtaatgcagg
    16621 caaacgagcc ctgaaacgcc acgtggatat actgataaat gctgcccatc accgccgtca
    16681 gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa gaagttgttg ccctcaccaa
    16741 acgccagcga gtaaccgtaa accacccaga gaatacagac cagtgcaaac gtcaccgtca
    16801 cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa cccaccgtaa aacagggcaa
    16861 tccccggaat agtcataaac agcaccagcg cagtacaaat catcataaac gcattgtcgg
    16921 ctttatcggc caccgcaggt gcagccatta ccagtcccgg cagcatcgcc agtgaagcaa
    16981 gcccagtttt tatcgtcgct atcttcattt ttcgttcctg ttgctgtgtg ccagagatta
    17041 cagcgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca attcagcgac
    17101 gaagattttg ccgtcgccaa tttttccggt gtaagccgcc ttactgacga tatcgatcac
    17161 ttcatcgagt tggtcatcag caatcgccac atcaattttt acttttggca ggaagttgac
    17221 gctgtattcc gccccccggt acagctcggc atgccctttc tgacgcccga aacctttcac
    17281 ttcggtgacg gtcaggccct gaataccaat ggaagataac gcttcacgaa cgtcttccag
    17341 cttgaatggt ttgattatca cggtcaccag cttcatagat cccctccggt cagaattcgg
    17401 taatggttct gctacacgaa gaaggtattg caagcggtgt gccagaaatg aaaaacgaga
    17461 aagagagcag gaagttaaga agattgaaaa tgaaaacgca ctatgacagt gcacagtgcg
    17521 ttacattttt gcaccaacga tgaaatattg cgctattcag gcgctcaatg attcttcttc
    17581 acgcacgctg gctgccagct cttcgcccgc aagttgcagt tgatacatct gccagtagcg
    17641 tccctgggcc gccagcagtt gctggtgagt gccctgctcc acggcttgcc cacgatgaag
    17701 caccagaatg gtgtcggcat caacaatggt cgataagcgg tgagcaatca ccaccagcgt
    17761 ggtatgttca cgcacagccg ccagagcatg ttgaatcgcc tgttcagtac cggagtcaat
    17821 gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccaaca cgcgtgccag
    17881 tgccagcagt tgcttttgcc cgacggagag attattcccc tgctcgccca gcggcgtata
    17941 aataccatca ctcatgctgc gcgccagctc cgccagttgc acggtttcca gcgcctgcca
    18001 gacgcgttct tcggagatat cacgccccag cgtcacgttg gcgaggaagg tatccgccag
    18061 caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctgt gacttagcga
    18121 acttaatgga cgaccatcaa ggcgaatctc accttccgtt agcgggtaat agcccatcaa
    18181 taaactggcg agggtgcttt tgccactgcc ggtatgcccg accagtgcca caaagttacg
    18241 cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat aagcaaatga
    18301 cacgttatcg acttcgatgg tgccactctg taacgggcga tcatcattgc catattgctg
    18361 gcgcggtccg tccatcagtt caaacacgcg ctcaccagca acaacagcct gttgcagcat
    18421 cgcctgttgc gtggtcagtt cgattaatgg ttcgttaagt cgcccaagat agctgataaa
    18481 cgcatacagc acgcccactt caatggtgcc gctagcggag aagccaaaca gcatcaacaa
    18541 gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa aaccgtcgag
    18601 gcgcagggtt tgcatcctgg ccatatagtg tgaacgactg gcctccccca tacgttcgcc
    18661 aaatcgcgcc tgctgacgga actgctggat aacgctcatg ccattgatga tttcgttaaa
    18721 gccgtcgttg atatccgcta gataggcgcg cacacgacgg acaatcggcg tgctgtaacg
    18781 ctggtatatc accattacca ccagcaccac cgggaaaatc attatcgcca ccagcgccat
    18841 tcgccagtcg aggctgaaca tcgccaccag catcgcgccc accagtgcgg cgctacgcag
    18901 gacagttgcc actacggtaa cgtagagatc gcggatcact tcagtgtcat tagtgacgcg
    18961 ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc gcaatgcggc
    19021 atccatcacg tcggtacgca actgttgcac tacgcctact gccgcccgat taaacagcag
    19081 cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc ccaacatacg ccgcagccag
    19141 ccctgcaacc attttcaacg gcaggttatt tttcgctacc atattgtcga taaaatagct
    19201 gataagcagc ggcccactga cttctgccgc tgccgcaacc cacatcatca ggaccgcaat
    19261 ccccagcggt ttacgccacg gcgaaccgta cgctaacaga cgcttgagag tcggccacag
    19321 ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc cggagcgtcg tcgagcgccg
    19381 cctccagttg ttgatagcga tacatatcgc gataccagcc gctttgttgc gccagctcat
    19441 catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca ctggcttccg
    19501 tcagtgccga aaggcgatgg gcactgatga ttaccgttct accctgtccc cactgacgca
    19561 ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc cgaaagcgca tcatcaagga
    19621 tgaggatttc cgcgttgact aataacgcac gagcaataga gatacgctgt ttttgcccgc
    19681 cggaaagcat cacgccgcgc tcgcccacct ctgtatcgta accttgcggt aaacgcaaaa
    19741 tatcgtcatg tacgctggct aaccgcgcga catgctcaat ctcttgctgg gtggcattcg
    19801 ggcaacccaa cgcgatgttg ttcgctacgg tgtcggaaaa aaggaacggt gtctggctaa
    19861 ctaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga atatcatgaa
    19921 agcgaatatc cccctcgctg acgtcgaaat gacgctgaat gagcgacaac agggtacttt
    19981 tgccggaacc agtcggcccg cagataccca gcatctgacc gggtttcagg gcgaaattga
    20041 cgttttccag cgcacgatgg tcagtctgcg gatacgtgaa ctggtgaata tttacatcaa
    20101 gttcgccacg cccttccggc acgggttcgc taccatcgtt caccaccggc gcttccgcca
    20161 gcatcgcacg aatacggctg tacgcagcac taccacgttc cacaatgtta aacatccatg
    20221 ccagcgccag cattggccaa atcatcagac ctaaatacat cataaaactg gtgagctggc
    20281 ccagcgttaa actgccctgc accaccatcc aactaccgcc gccaatcgcc agcaagttcg
    20341 ccataccaat cgcgatatag attgtcgggt cgaaacgagc atcaatacgc gccacccgca
    20401 tgtttttttt gccggtatct tcggcatccg cggcaaataa cgccgactgg cgatcttcca
    20461 gaccaaaggc tttgatcatg cggatactgg tgaggctttc ctgggtgcgg tcattaagac
    20521 tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa ggcatcgccg ttgcgcttaa
    20581 tcatgatcgc catcactggc atcggtaaca gggcaaataa ggtcaactgc cagctaattt
    20641 gcgtagacat cataatcaac acagcgcagc ccattaccag tgaatccacc agcgtcagca
    20701 ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt ggtcgcacga gccatgagat
    20761 cacccgtgcg atgacgcagg taaaactccg ggtgctgccg gcttagctga cggtaataat
    20821 cttcacgcag ttcaacagcc agttggtaag acgcgccaaa cagcaatacg cgccagacat
    20881 aacgcaggag ataaaccaca acggcaatca gcaccatggt ggcgatccac atcaggatca
    20941 gcccggtagt aaagtgttgt tctgtcacgc catcgacaac aataccaacc acttttggcg
    21001 gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac agccccgaga tagcgacgcc
    21061 attcccgacg gaaataccag cttaattgag caaataatcg cacgcgttgt gtcctgacct
    21121 gattctggat atttattcga tgggtaaaga agtggtgtat ttaatctgtt ccatcgcgaa
    21181 gctggaagtg acgtccgaca gccccggcac gctgtttacc agacgcttat aaaactcgtc
    21241 gtagcgtttc atgtcggcaa cctggacgcg catcagataa tcgtattcac cagccatgcg
    21301 ccagaacccc agcacttctg gcatttcggt aaccaccgtg acaaagcggc aataccattc
    21361 gctgctgtga tgttgcgttt ttatcagcac aaaagcggtc aggccgaggc ctattttttc
    21421 cggatccagc agggcgactt tgccgataag gataccgtcg tcctccaggc gtttcagacg
    21481 cttccagcaa ggggtggttg tcagattaac ggcttcagcc agtgcctgca aagagagggt
    21541 gcaatcctgc tgcagtaagg ccagcagctt atggtcaatt ttatctaaca tagcccttcc
    21601 acagagaatt tttttctctt ttaattctat tttaaaggtg aaatcacgac aatttattct
    21661 gtgcggttta aacaccacgg cacaaaatga caaattcaac gcaaaaaaaa ccgggcaatt
    21721 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    21781 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    21841 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    21901 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    21961 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    22021 tcgaggcaat ccgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    22081 tggatctgca agcgtgtaag atcgtcgtga ccgccacaga agcagatctt ggtgactctg
    22141 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    22201 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    22261 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    22321 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    22381 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    22441 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    22501 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    22561 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    22621 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    22681 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    22741 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    22801 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    22861 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    22921 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
    22981 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    23041 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    23101 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    23161 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    23221 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    23281 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcatattg
    23341 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    23401 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    23461 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    23521 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    23581 aaaaagattt gggaaacagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    23641 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    23701 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    23761 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    23821 tactgccagg ctggactatt ccgtactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    23881 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    23941 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    24001 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    24061 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    24121 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    24181 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    24241 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    24301 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    24361 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    24421 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    24481 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    24541 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    24601 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    24661 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtccctggg
    24721 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    24781 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    24841 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    24901 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    24961 gatttagtcc gtaacgaaac gttgacctgc tataacggct ttaaaggcga cggttgcggt
    25021 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    25081 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    25141 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    25201 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    25261 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    25321 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    25381 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    25441 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    25501 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    25561 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    25621 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    25681 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    25741 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    25801 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    25861 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    25921 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    25981 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    26041 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    26101 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    26161 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    26221 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    26281 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    26341 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    26401 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    26461 gcttaagatt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    26521 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    26581 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    26641 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    26701 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    26761 aaccggcttg aagttcaact cttccggcag gttatctttg ctaaaccaac ccgtctgagt
    26821 ggctttaacg ccggcagctt gctctgcacc ggccagagac tccgtgtcgt tgcttgccgc
    26881 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    26941 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    27001 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt gccctttttc
    27061 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    27121 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    27181 accgccttta ttcagctcat caagtaccac tttcgcttca tcttcagttt tggtctggat
    27241 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    27301 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcatccagct tgatgtaact
    27361 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    27421 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    27481 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    27541 atcggtaccg gcaacaccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    27601 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    27661 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    27721 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    27781 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    27841 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    27901 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    27961 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    28021 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    28081 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    28141 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    28201 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    28261 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcagcagc gatggtgatc
    28321 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    28381 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    28441 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    28501 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    28561 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    28621 ttaataaggg caagcccgat ccgccatcta acttagcgag acaaaaaaag gctggcaagc
    28681 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    28741 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    28801 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    28861 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    28921 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    28981 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    29041 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    29101 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    29161 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    29221 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    29281 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    29341 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    29401 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    29461 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    29521 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    29581 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    29641 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    29701 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    29761 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    29821 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    29881 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    29941 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    30001 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    30061 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    30121 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    30181 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    30241 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    30301 tttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    30361 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    30421 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    30481 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    30541 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    30601 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    30661 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    30721 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    30781 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    30841 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    30901 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    30961 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    31021 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    31081 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    31141 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    31201 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    31261 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    31321 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acatcttcca
    31381 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    31441 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    31501 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcgtttt
    31561 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgcgacaa
    31621 ccggcagacg accaataaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    31681 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    31741 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    31801 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    31861 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    31921 cctgctgtac gccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    31981 aaatcttgtc gatctcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    32041 cgcacttctg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    32101 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    32161 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    32221 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    32281 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    32341 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    32401 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    32461 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    32521 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    32581 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgccctg
    32641 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    32701 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    32761 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    32821 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    32881 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    32941 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    33001 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    33061 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    33121 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    33181 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    33241 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    33301 cgtctcctgg ataaaattga aaaaacctgc tgtaccgatt gtaaccttat ggacggatta
    33361 tcagctagtc cctgtgttat gggtacggca tcaccatatt tcaagcataa caatcttttg
    33421 ttgttacgct aacaccgaaa gaggcttttc gcacggttcc atgcaaaatt catgacaaaa
    33481 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    33541 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    33601 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    33661 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    33721 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    33781 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    33841 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    33901 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    33961 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    34021 catgttttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    34081 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    34141 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    34201 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    34261 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    34321 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    34381 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    34441 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    34501 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    34561 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    34621 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    34681 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    34741 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    34801 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    34861 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    34921 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    34981 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    35041 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    35101 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    35161 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    35221 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    35281 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    35341 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    35401 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    35461 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    35521 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    35581 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    35641 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    35701 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    35761 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    35821 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    35881 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    35941 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    36001 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    36061 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    36121 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    36181 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    36241 atcgtgccag ctacaatgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    36301 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    36361 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    36421 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    36481 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    36541 tggatgacgg tcgagaatat tgatctcaaa accattggtt tcttctctct ggtaggccag
    36601 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    36661 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    36721 atgggttttc tcgaacctgg cacccaactc cgctggatgg cggcactggc ggtggtgatc
    36781 gctttttgct cggcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    36841 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    36901 gtttccggcg gcttagccct gtggctggca gataaatggc tgggctggca gggcatgtac
    36961 tggttgatgg cggcgctgtt gatcccttgt attatcgcga cgttgcttgc accagaaccg
    37021 accgacacca ttcctgtgcc aaaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    37081 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    37141 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    37201 ggtgaagtag gcgtggttaa caaaacgctt ggcttactgg cgaccattgt gggcgcattg
    37261 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    37321 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    37381 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    37441 gtcgcgctgt taatgacgct gtgtaataag tcattttccg caactcaatt tgccctgctc
    37501 tcagcgcttt ccgctgtagg acgagtttat gtcggccccg tggcgggttg gtttgttgaa
    37561 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    37621 ttgctgctgg tttgccgcca gacgcttgaa tatacaggag taaatgacaa ctttatctcc
    37681 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    37741 ctgttggccg tgtggttact gctgttgacg atggacgcgc tggatttgac gcacttctct
    37801 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    37861 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    37921 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagt atcgcttaat
    37981 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tgacgcaatt tatgaattct
    38041 attttttctt acacgattca gctaatgagt ctttattttc ccccacccaa ttgtcaccat
    38101 aatgataatt atttgttaaa caattgtttt attccagatt ggttatacca attgcccgtc
    38161 caaactcctt ctgcactccc ctttcgttat aacgcccttt tgcaacagct tcttaaaatc
    38221 aacttgatat gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta
    38281 atcatgttta cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag
    38341 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    38401 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    38461 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    38521 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta tcgtgcgagc
    38581 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    38641 tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac
    38701 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    38761 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgctac cgtgaatgaa
    38821 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    38881 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    38941 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    39001 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    39061 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    39121 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    39181 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    39241 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    39301 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    39361 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    39421 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    39481 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    39541 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc
    39601 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    39661 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    39721 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    39781 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    39841 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac
    39901 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    39961 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    40021 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    40081 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    40141 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    40201 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    40261 ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc
    40321 gtttggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
    40381 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    40441 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc
    40501 actttctcgg tgggcgggat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    40561 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    40621 ttcggctgct tcgcagggat gacctactgg tggcctaaag ccttcggttt caaactgaat
    40681 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    40741 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    40801 cagttccaca ccatgctgat gattgcagct agcggtgcag tactgattgc gctgggtatt
    40861 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ccgtgacctg
    40921 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    40981 tataactttg ccgttgtgcc gcacgttcac gagcgtgatg cattctggga aatgaaagag
    41041 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    41101 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    41161 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    41221 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    41281 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    41341 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    41401 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    41461 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    41521 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    41581 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    41641 tgccggattt atcgggatgg aaatctacga attccatcac ctgattgtta acggcatggg
    41701 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    41761 cgtcacttcc ggtcttatct ggatggcggt gctgatggta caaatcgctc gtcgcggcct
    41821 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    41881 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    41941 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    42001 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    42061 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    42121 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    42181 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    42241 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    42301 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    42361 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    42421 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    42481 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    42541 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    42601 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    42661 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    42721 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    42781 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    42841 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    42901 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctggat
    42961 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    43021 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    43081 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    43141 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    43201 attgtctgag atcagagata cggctaataa ccgtatgtct tgacagagtg agcagatgaa
    43261 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    43321 attcagttaa tcaaagaggt cataatgcct ggaaaaacag ccactctacc cgatgtcgat
    43381 aaaacgctga aaaatgcgcg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    43441 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgctgctatg
    43501 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    43561 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    43621 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    43681 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    43741 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    43801 tgcggctaaa gcacttattg acagacaaag tggtgaactg atcggcatct gtacttttac
    43861 ggcatattcg ctggaaaaac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    43921 aattggtgtt gtcagattag tcatgttggg ggtagcacgg aagtatcaaa agcggggctt
    43981 tggtcaggac ctactatgtg atttttttga acatgtaaaa ataattcacc aggcattacc
    44041 aattaaaggg gtttatcttg atgctgaccc tgccgccatt aatttttatg ctcgtctcgg
    44101 ctttgttcag ctttcagcga caccaaatgc ttttggtgct gtacctatgt ttttggcgat
    44161 tcagcatatt ctcgcggctt agattattat cgccacccgc cagggcaagt tttgcctcag
    44221 ccgggggctg agcagcgtta ccacttttgt tcaattgccc ccgctttcag caactccaca
    44281 tccttgccgt taatggttgc cgttgcggca acgattgtgg tccttacagt tgcacaagat
    44341 tttcgctcaa attccggttt ataaagaata agagacgaat tactggtaaa tgaaccaaat
    44401 aggtaatcag taaagctaat attcagccgg tttaaaccga aaggcgcgaa gccaaccgtt
    44461 tggttaatct ccttaccgga tgggcgacga atgctgaatg tcacgcgaag attggccact
    44521 tcgcctaatt tattggtggt ggcgaagagg ctatccatgc aggaaaaacc tttatctaaa
    44581 aaattatctc gcatcccaga acgggtaacc gtaatcacgg gtaacgtatc gtcaaccgtt
    44641 tttctgtcaa tcatgtcttt tcgacaagta gccagaaagt cttctgggcc accataaatg
    44701 tggacacctt tatcaagata ttccttcgct ttcttacaat ctacctcaac atactgacca
    44761 ttggcgtaca taatgcccaa cctggacctt gctaaattca caccgtgctg cgcggcctga
    44821 ttaaatagtg catacgcttt ggcataatcc acccgcacct gctcaccgtc gtagtagata
    44881 ccccccagcg ctaacggagc ttctctgtca ccttgtttag ccactaactc gaaccattta
    44941 atcgcttcct gaacatcagg tttggtatca taacgaggct gataatattc ccagcctaac
    45001 aggctctgcg cgtcgatatc tcccgattca gccattttat gcagtgtatt tataacatcg
    45061 ggagcaggtt taccaccgta tatgctgtca tacaattcat gcgctttctc gctggccacc
    45121 acgcttggtg aaactatcag cattacagaa aataatattc ttttagttaa catgtatacc
    45181 tttatgtcct cactgtcata tatttccata ataattaacc accaaatatc catttaatga
    45241 ataaatacag tgccataaag gtccaagtaa taagcacaca tttagcctga aacattatca
    45301 tccatgtcac caaatcgttt atttagtgag ataaaactag catattcgta agataaaaaa
    45361 tgcacatagc aatcaagaaa tgatattatt acttttttga tgtaaaacta tttggtttta
    45421 aaattcattt tttcaacata tagagcattc attttatgaa tgcaaaaaaa catacctgac
    45481 acacaaatta ctctcctctg taatattttc tcattaacac tctgtttacc caacatgcgc
    45541 tccgcactac actatgtggg cattttttta aggggtggta atgaacgatt ataaaatgac
    45601 gccaggtgag aggcgcgcga cctggggttt agggaccgta ttctcgttgc gcatgctggg
    45661 catgtttatg gttctgccgg ttctgaccac gtacggcatg gctctgcaag gtgccagcga
    45721 agcattaatc ggtattgcca ttggtattta tggtctgact caggccgttt ttcagattcc
    45781 gtttggcctg ctttccgacc gcattggtcg caaaccatta attgtcggtg ggctggcggt
    45841 gtttgccgcc ggtagcgtta tcgctgcgct ttccgactcc atctggggaa ttattctggg
    45901 ccgggcgcta caaggctctg gagcaattgc cgccgccgtt atggcgctgc tttccgatct
    45961 cacgcgcgaa caaaaccgca ccaaagcgat ggcgtttatc ggcgtgagct ttggcattac
    46021 ctttgccatt gcgatggtgc ttggcccgat catcactcac aaacttgggc tgcacgcgct
    46081 gttctggatg atcgctattc tggcaacaac cggcattgcg ttgaccattt gggttgtacc
    46141 caacagtagc actcacgtac ttaatcgtga gtccggaatg gtgaaaggca gtttcagtaa
    46201 agtgctggcg gaaccgcggc tgctaaaact caactttggc attatgtgtc tgcacatgct
    46261 tctgatgtct acgtttgttg ccctgcccgg acagctagct gatgcggggt tcccggcggc
    46321 tgaacactgg aaggtctatc tggcgacgat gttaatcgcc tttggctcgg tcgtgccttt
    46381 cattatctac gctgaagtta agcgcaaaat gaagcaagtc tttgtcttct gcgtcgggtt
    46441 gatcgtggtt gcggaaattg tgttgtggaa cgcacaaacg cagttctggc aactggtggt
    46501 cggcgtgcag cttttctttg tggcgtttaa tttgatggaa gcccttctgc cctcacttat
    46561 cagtaaagag tcgccagcag gttacaaagg tacggcgatg ggtgtctact ccaccagcca
    46621 gtttcttggc gtggcgattg gcggttcgct gggcggctgg attgacggca tgtttgacgg
    46681 tcagggggta tttctcgctg gcgcaatgct ggccgcagtg tggctggcag tcgccagtac
    46741 catgaaagaa ccgccgtatg tcagcagttt gcgcattgaa atcccggcgg acattgccgc
    46801 aaacgaagcg ttaaaagtgc gtttgctgga aactgaaggc gtcaaagaag tgttgattgc
    46861 agaagaagaa cattcagctt atgtgaaaat cgacagcaaa gtgacgaatc gctttgagat
    46921 agaacaggca attcgtcagg cataaaaaac gcggcgaatt gcctgctgcg ctacgcttat
    46981 caggcctaca aagtttctga attacaaacc tttgtaggcc gaatcaggcg ttcatgccgc
    47041 atctgacatg aacaaaacgc acatagtcgc gattaatcgc ggaagttttt gaactggaac
    47101 ggctgaccga gatcgccacc acgtaccatc gccatgacag cctgcaaatc atcacgagat
    47161 ttgcccgtta cgcggatttc atcgccctga atttgcgcct gcactttcag tttgctgtct
    47221 ttgatcatct tgacgatttt cttctgggtc gcgctctcaa tgccctgttt cagtttcgct
    47281 tccacaaacc aggttttacc gctatgaacg atattctccg gtacatccag cgaactgcct
    47341 tcaatgccgc gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaaa
    47401 tcggactcgc tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca
    47461 acgttacgga agtcaaaacg ggactccact tcgcggctcg cgttatcgac cgcgttacgt
    47521 gcttcctgaa gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc
    47581 atttttgatg cgaagcataa tacccgcaaa gttaaataac cgacagtcct gcaccaggac
    47641 gtatccctta attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa
    47701 cacccggagt ggttgcgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc
    47761 cttagggcaa ttatggctta cagcactttg caaacagggt catgaagttc agggctggct
    47821 gcgcgtaccg caaccttatt gtagcgtgaa tctggttgag acagatggtt cgatatttaa
    47881 cgaatcgctg accgccaacg atcccgattt tcttgccacc agcgatctgc tcctggtgac
    47941 gctgaaagca tggcaggttt acgatgccgt caaaagcctc gcgtccacac tgcctgtaac
    48001 cacgccaata ctgttaattc acaacggcat gggcaccatc gaagagttgc aaaacattca
    48061 gcagccatta ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat
    48121 tcatgtggca aacggaatca cgcatattgg tccggcacgg caacaggatg gcgattacag
    48181 ttatctggcg gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg
    48241 cgccgagctg tggcgcaagc tggccgtcaa ctgtgtgatt aatccactga ccgccatctg
    48301 gaattgcccg aatggtgaat tacgtcatca tccgcaagaa attatgcaga tatgcgaaga
    48361 agtcgcggca gtgatcgagc gcgaagggca tcatacttca gcagaagatt tgcgtgatta
    48421 cgtgatgcag gtgattgatg ccacagcgga aaatatctcg tcgatgttgc aggatatccg
    48481 cgcgctgcgc cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc
    48541 gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag
    48601 tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg
    48661 tcaccactat cgatctgctg gttcgcggcg gtatcaaagt caccactgcc agcgtcgcca
    48721 gcgatggcaa cctggccatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc
    48781 tggtcgaggt ggctgatggc gaatatgacg tgatcgtact gcctggtggc attaaaggcg
    48841 cagagtgttt tcgcgatagt accctgctgg ttgaaaccgt taaacagttc caccgttccg
    48901 ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct
    48961 tcccgattgg taatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat
    49021 ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg
    49081 ggacagctat cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc
    49141 atgaagtggc atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc
    49201 ggatgcggta agcgtcgcat ctgaccagat gcgacgttac aaccacaaat tacgggcgat
    49261 ataccttcac attgttaaag ccctgctcgc gcagatagag cgcctgcaga cggctcatca
    49321 ccccgcgctc acaccacagt agccaggttc tgttctggtc gagatcgcca aacttggtgc
    49381 tcagtttata gaacggcaga gaaactacat caatcccttc gactttcagt ggcttatctt
    49441 cctgttcatc gatagaacgg atatcgagga tcacgtcgtt cgggccgaag ccattgacgg
    49501 tttccacttc caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat
    49561 tcgcttcttc aaccacttta tcgagaatgc tgaagtcgaa tttctcttct tccgcttcaa
    49621 tcttcgactt aaccgctttc accgtcgggc ttttggagat aacaccgcaa tattccggca
    49681 tcgtgcgagc aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt
    49741 cgtaagaaat cagcggacgc aggatcagcg tatcggagac gttatcaatc aggcgcaggt
    49801 tggtcagcgt ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc
    49861 cgtaacgttc agccacttta gacgcggcac gcaccatcat acgtttgagg ataacgccca
    49921 tctgaccgtc gtcgattttc tcgagaattt ccccgacgac cggttcgaaa ttaatagcga
    49981 caaaacgcac gcggtgggag ctgccaaaac ggttccacag ataatgcgcc acctgacgca
    50041 cgccaatttc atgcgccgcg ccgccaaggt taaagaaaca gtaatgcacg cggcagccgc
    50101 gacgcatcaa catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca
    50161 catcttcctg ggtgccgatc gggaaaccgc caataccttc gtagcggcct ttaatcagca
    50221 ggagacgatc gtcttccact tccagatgga cagtcacttc cggattggtc agcttcacgc
    50281 gcgcggactc aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc
    50341 taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat
    50401 cgcgatactg aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt
    50461 cgacttcgag aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagccagac
    50521 gctggttttc atcttttgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg
    50581 tctcatcata gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca
    50641 agcgcacaga ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg
    50701 cggcaatggt tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac
    50761 acctgcggct gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca
    50821 ccttaccatt gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa
    50881 atattgagag tcagacattc attatgccga agaaaaatga ggctcccgcc agctttgaaa
    50941 aggcgctgag cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg
    51001 aagaggcgct gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat
    51061 tacaacaagc cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa
    51121 ccccttttac accggacaat gagtaatgga ctttccgcag caactcgaag cctgcgttaa
    51181 gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt
    51241 ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgtctgcgac ccttcctggt
    51301 ttatgccacc ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgccgc
    51361 cgtagagtgt atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga
    51421 cgatctgcgt cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct
    51481 cgctggcgac gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga
    51541 agtgtcggat cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc
    51601 cggaatgtgc ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga
    51661 cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct
    51721 tggtgcatta agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtacgc
    51781 agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac
    51841 tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc
    51901 acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca
    51961 gtcgctgaaa caactggctg aacaatcact cgatacctcg gcactggaag cgctagcgga
    52021 ctacatcatc cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga
    52081 tattgccaaa tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc
    52141 gaaagagagt ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag
    52201 ccgttccagc gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca
    52261 ctatgtctac aacaccccgt ttgaccaatt gatttgggat gtggggcatc aggcttatcc
    52321 gcataaaatt ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggcct
    52381 gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc
    52441 aacctccatc agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg
    52501 ccgtaccgtc tgtgtaatcg gcgatggcgc gattaccgca ggcatggcgt ttgaagcgat
    52561 gaatcacgcg ggcgatatcc gtcctgatat gctggtggtc ctcaacgaca acgaaatgtc
    52621 gatttccgaa aatgtcggcg cgctcaacaa ccatctggcg cagctgcttt ccggtaagct
    52681 ttactcttca ctgcgcgaag gcgggaaaaa agttttctct ggcgttccgc caattaaaga
    52741 gctgctcaaa cgtaccgaag aacatattaa aggcatggta gtgcctggca cgttgtttga
    52801 agagctgggc tttaactaca tcggcccggt tgacggtcac gatgtgctgg ggcttatcac
    52861 cacgctgaag aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa
    52921 aggtcgtggt tatgaaccgg cagaaaaaga ccccatcact ttccacgccg tgcctaaatt
    52981 tgatccctcc agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat
    53041 ctttggcgac tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc
    53101 ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt aaattcccgg atcgttactt
    53161 cgacgtggca atcgccgagc aacacgcggt gacctttgcc gccggtctgg cgattggtgg
    53221 gtacaaaccc attgtcgcga tttactccac tttcctgcaa cgcgcctatg atcaggtgct
    53281 gcatgacgtg gcgattcaaa agcttccggt cctgttcgcc atcgaccgcg cgggcattgt
    53341 tggtgctgac ggtcaaaccc atcagggcgc ttttgacctc tcttacctgc gctgcatacc
    53401 ggaaatggtc attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg
    53461 ctatcactat aacgacggcc cgtccgcggt gcgctacccg cgcggtaacg cggttggcgt
    53521 ggaactgacg ccgctggaaa aactgccaat tggcaaaggc attgtgaagc gtcgtggcga
    53581 gaaactggcg atccttaact ttggtacgct gatgccagac gcggcgaaag tcgctgaatc
    53641 gctgaacgct acgctggtcg atatgcgttt tgtgaaaccg cttgatgaag cgttaattct
    53701 ggaaatggcc gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg
    53761 cgcaggcagc ggcgtgaacg aagtgctaat ggcccatcgt aaaccagtac ccgtgctgaa
    53821 cattggcctg cctgacttct ttattccaca aggaactcag gaagaaatgc gcgccgaact
    53881 cggcctcgat gccgccggta tggaagccaa aatcaaggcc tggctggcat aatccctact
    53941 ccactcctgc tatgcttaag aaattattca tagactctaa ataattcgag ttgcaggaag
    54001 gcggcaaacg agtgaagccc caggagctta cataagtaag tgactggggt gaacgaatgc
    54061 agccgcagca catgcaactt gaagtatgac gagtatagcg ggagtggcag catgcaatac
    54121 aaccccttag gaaaaaccga ccttcgcgtt tcccgacttt gcctcggctg tatgaccttt
    54181 ggcgagccag atcgcggtaa tcacgcatgg acactgccgg aagagagcag ccgtcccatc
    54241 attaaacgcg cgctggaagg cggcataaat ttctttgata ccgccaatag ctattccgat
    54301 ggcagcagcg aagagatcgt tggtcgcgca ctgcgggatt tcgcccgtcg tgaagacgtg
    54361 gtcgttgcga cgaaagtgtt ccatcgcgtt ggtgatttac cggaaggatt atcccgtgca
    54421 caaattttgc gctctatcga cgacagcctg cgccgtctcg gcatggatta tgtcgatatc
    54481 ctgcaaattc atcgctggga ttacaacacg ccgatcgaag agacgctgga agccctgaac
    54541 gacgtggtaa aagccgggaa agcgcgttat atcggcgcgt catccatgca cgcttcgcag
    54601 tttgctcagg ccctgaagct acaaaaacag cacggctggg cgcagtttgt cagtatgcag
    54661 gatcactaca atctgattta tcgcgaagaa gagagcgaga tgctgccact gtgttatcag
    54721 gagggcgtgg cggtgattcc gtggagcccg ctggcgcggg ggcgactgac gcgtccgtgg
    54781 ggagaaacta ccgcacgact ggtgtctgat gaggtgggga aaaatctcta taaagaaagc
    54841 gatgaaaatg acgcgcagat cgcagagcgg ttaacaggcg tcagcgaaga actcggtgca
    54901 acacgagcac aagttgcgct ggcctggttg ttgagtaaac cgggcattgc cgcaccgatt
    54961 atcggaactt cgcgcgaaga acagcttgat gagctattga acgcggtgga tatcactttg
    55021 aagccggaac agattgccga actggaaacg ccgtataaac cgcatgcggt cgtaggattt
    55081 aaataagagt ccaggcctga tgagacgtga caagcgtcac atcaggcatc ggtgcacaac
    55141 tacgacagaa tacccagcgg ccagtgatga ccgataaagt acaggatgcc tgcggaaatc
    55201 accccggcga caatatcgtc gatcatgatc cccatgccgc catgtacatt gcgatcaaac
    55261 cagcggatcg gccacggctt ccacatatcc agaatacgga aaatcacaaa cccggcggca
    55321 acccactgcc agtcattggt cggcagcgcc atgagcgtga tccacatacc aataaattcg
    55381 tcccagacaa tgctgccgtg atcgtgcacg cccatatctt tcgccgtttg atgacaaaga
    55441 tagacgccga tacagatccc caacatcacc accagcgagt agagctgcca gggtaaaaag
    55501 gtcatcagat accagaacgg aatcgctgcc agcgatccca tcgtgccagg aacgattggg
    55561 cttaatccac ttccgaatcc gacagcaagc agatgccacg gattactcat cttcaggcga
    55621 cttttcgcga catctttatg gcgtggcaaa atggtcatat cctttccagt caaacgtgac
    55681 aggttcgccg tcacgaataa aacaaagccc ttcgatatcg gcggtcattt gcccgataca
    55741 ggtaaacggt acgcccaggt gtccgagagc cacatccagc gcgccacggt tcagttccgg
    55801 cacagtgaaa cacaactcgt aatcttcacc gccagagagc gcccagcgca gcgcctgttc
    55861 cggttcaaca tggcgagaaa gcgcatcaga aaacggcagc aatgccaggt caatacgtgc
    55921 gccgcagtcg ctggctttca cgatatgccc gagatcggaa atcagaccgt cagagagatc
    55981 gatagctgaa tttgccagat cgcgcagtgc ctgcccctgt aaaatacgcg gcgatggacg
    56041 gagatgacgt ttgatcaagt agtcagcatc tttagcatcg gcaacctgca aacggttttg
    56101 caaaatcgcc agcccggcgg cgctatcgcc cggtgtaccg gtcacataga tccagtcacc
    56161 cggtttcgcc ccagagcgcg ttaaggctcg tcccatcgga acaaagccgt ggatacccaa
    56221 cgtcattgat aatggcccac gcgtggtatc gccgccaatg agttgcatat cgtaataatt
    56281 gagaagatca aacaaactgt cgctgaagga ctcaagccac gcttcgtcta cgtccggtaa
    56341 ggttaatgcc agcgtcagcc aggccggatc ggcccccatc gctgccagat cgcttaggtt
    56401 cactgccagt gctttatacg ccagatcagc aggatcgata tcatggagga agtggttgcc
    56461 cgccaccagc gtatcagtgc tgatcgccag ggtctgtttc tcggggatat tgagaagagc
    56521 gcaatcgtcg ccgatgccca gttcgacatc aagacgagaa cttcttacac ggtcaaaata
    56581 acgggcaatc agggagaact cgccacatgc catacgttat gcctcagcag gtaaaaaaga
    56641 aaaggccggc gatcgcggaa cggtcttccg tgaatctacc ggcctggata tcactttttg
    56701 ttagggcgaa tcacaggtgc tgctttatcg agtacgccgt tgacgaactt atggctgtct
    56761 tctgcgccga acgatttcgc cagttcgatc gcttcgttaa tggccacttt gtatggcaca
    56821 tcgctacgtt tagacagctc gtacagcgca atgcgcagta ctgctttttc tacctgtccc
    56881 agttcttcca gcaggcggga caggtatggc ttcatcagtc cgtcgaggta tgcggtgtta
    56941 gtcgccaccc cggccagcag ctcacggaag tacaggacgt caacgtcttt tacatcctgt
    57001 tcagccagga actggtattc aacatcagcg atgtcgttct gggacaactg ccaggagtag
    57061 agcgcctgga cggcacactc acgagcgcgg cgacgagcag caggtttcac ggatttcccc
    57121 ttactaattt caggccttga tggctttcaa tacattaatc atttcaagcg cggtcagtgc
    57181 agcttctgca cctttgttgc cagctttggt gccagcacgt tcgatcgctt gttcaatgct
    57241 ttcagtggtc agaaccccaa aagcaaccgg aatttcgctg tcctgggcaa catgcgccag
    57301 gccgttgctt gcaccaccag cgacatattc aaagtgggca gtgccaccac gaataaccgt
    57361 acccagcgca atcaccgcgt cgtatttacc ggttttagcc agtgcacccg ccgccagcgg
    57421 cagctcatag gcaccaggca cccaaacaac ggtaatgttt tcatctttta cctgaccgat
    57481 acgtttcagt gcgtcaattg caccttccag caggctgtca ttgataaagt tgttgaaacg
    57541 cgcgatggtg atggcgacgc gagcgtccgg ggtagcaacg ttagcttcaa taatgttcat
    57601 tttcttcctt cgggttcgag tatggccccg caggggggcg gattttagca taatatttcg
    57661 tgcgctgctt ccctttcgag ccgggagatc atgcacccac taaatgcagg caaacatccg
    57721 ggcctacatg acgtatctct ttgaatttaa attggggggc gtcggctaat ttctcaagcc
    57781 ctggcagcgt gcataatccg cgggcgtcgc tgcctaatag tttaggtgcg atatagacaa
    57841 tcagctcatc gactaaaccc gcctgcagca atgcgccagc gagcgttggc cccgcttcca
    57901 cccagatact gttaatttgc tgtttaccca gttgcatcat cagtacaacc agatccagat
    57961 gacctttatg ctctggaatc agcaaggtac gcaccgtttc cggccactca ctagaatctt
    58021 cctgcgtacg cgcgaaccag gtttcgccgg gctgctgcac aatgcgatgt tccggcgtca
    58081 cgcgattttg gctatcaatc acaatacgta ctggctggcg gagattttgt tgcgggtaga
    58141 gcgcctgagt ttgttcatcc agttcagacc aacgcactgt taaggctgga tcatccgcca
    58201 gcaccgtggc gctgctagtt aaaatggcat gactttgtgc gcgctgtcgt tgtacgtcgc
    58261 gccgcgcctg aggcgaagtg atccactggc tttcgccgct cgccatcgcc gtgcggccat
    58321 caagcgatgc gccaagtttt aactgaatat aaggaaagcc ggtgcgcatc cgcttgagaa
    58381 agcctttatt caattgctcg gcttcactca tcatcagccc gtggctgacg tcaatgccag
    58441 cctgttgcag acggtaaagt ccacgcccag cgacctgcgg gttaggatct tgcatcgagg
    58501 caaccacgcg cgctacgcca gcggcgatga gtgcgtcaca gcacggtggc gtacgaccat
    58561 gatggctaca gggttcgagt gtgacatagg cggtcgcacc tttggctttt tcacccgcca
    58621 tacgcaacgc gtgtacttcg gcatgtggtt cacccgcacg ttggtggtaa ccttcaccga
    58681 caatttcgcc atctttgaca atgacgcacc cgacattcgg gttgggatgc gtggtaaaac
    58741 gtcctcgttg cgccagcttt agcgctcgcg ccatgtaata ctcgtcctgc acggcttagt
    58801 cctccaggcg cgcgatctct tcgccaaatt ctttgatatc ttcgaaactg cggtagacag
    58861 aggcaaaacg gatataggcg actttatcga gctttttcaa ttgctccatc accagattgc
    58921 caatcatctt gctcggcact tcgcgctcac cggtggcgcg cagctgcgat ttaatatgat
    58981 tgattgccat ttcgacgtca tcggaactca ccggacgttt ttccagcgcc cgcagcattc
    59041 cgctacgcaa tttctcttca ttaaacggtt cacgcacgtc gttgcttttt acaacacgcg
    59101 gcataaccag ctccgccact tcaaaggtgg tgaaacgttc attacacacc agacactgcc
    59161 gacggcggcg tacggatgaa ccctcgccca cgagacgaga gtcaattacc ttagtgtcca
    59221 cggcgaaaca gaatgggcaa tgcatacggc gtcctgacca ggtggttaac agaaatctat
    59281 tttaccctga actgacgata caacaaaggc gcaacgcttt tgagataaag gatcgatgct
    59341 ttcgctgctt ttaccctcta ccattaggct aattgcgacg agaaagggac gacctatgaa
    59401 tactaacgtt tttcgactgc tcctgctggg aagcctgttc agccttagcg cctgtgtgca
    59461 gcagagcgaa gtgcgacaga tgaaacacag cgtcagcacg ctgaaccaag agatgacgca
    59521 actcaataaa gaaacggtca aaatcactca gcaaaacagg ctgaatgcaa aatccagcag
    59581 tggggtttac cttctgccag gagcgaaaac accggcaaga ctggaaagcc agatcggtac
    59641 tttacgtatg tcgctggtga atattacgcc tgatgcagat ggcaccaccc tgacgctacg
    59701 tattcagggc gagtcgaatg atcccttgcc cgcattcagc ggaactgttg aatatgggca
    59761 aattcaggga acaatagaca actttcagga aatcaatgtg cagaatcaat tgattaatgc
    59821 ccctgccagc gtcctcgccc ccagcgatgt tgatattccg ttacagttaa agggtatttc
    59881 tgtagaacag ttagacttcg tacgtatcca cgatattcaa cctgttatgc agtaaacgta
    59941 tttcggggcg attttgtgcg tctcgcaaca tctttccccg tcattttgtt actccgctta
    60001 catcaccctg attgatagta aaagtttgca acaagggtga aagtcagtac aatccctgcc
    60061 cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg atagaactgt gaaacgaaac
    60121 atatttttgt gagcaatgat ttttataata ggctcctctg tatacgaaat atttagaaac
    60181 gcaatttgcg cctttttcac tcccgcaagg gattttcaaa cagtggcata catatgaaaa
    60241 aaacattact ggcagccggt gcggtactgg cgctctcttc gtcttttact gtcaacgcag
    60301 ctgaaaacga caaaccgcag tatctttccg actggtggca ccagagcgtt aacgttgtcg
    60361 gaagctatca cacccgtttc ggaccgcaga tccgcaacga tacctacctt gagtacgaag
    60421 cattcgctaa aaaagactgg ttcgacttct atggttatgc ggatgcgccg gtattcttcg
    60481 gcggtaactc cgatgctaaa ggtatctgga accacggttc tccgctgttt atggaaatcg
    60541 aaccacgttt ctccatcgac aagctgacca atactgacct tagcttcggt ccgttcaaag
    60601 agtggtactt cgcgaacaac tacatttacg acatgggtcg taataaagat ggtcgccaga
    60661 gcacctggta catgggtctg ggtaccgaca tcgatactgg tctgccgatg agcctgtcca
    60721 tgaacgtcta tgcgaaatac cagtggcaga actatggcgc agcgaacgaa aacgagtggg
    60781 acggttaccg tttcaaaatt aaatactttg tgccgattac cgatctgtgg ggcggtcagc
    60841 tgagctacat cggcttcacc aacttcgact ggggttccga tttaggggat gacagcggta
    60901 acgcaatcaa cggtattaag acccgtacta ataactctat cgcttccagc catattctgg
    60961 ctctgaacta cgatcactgg cactactctg tcgtagctcg ttactggcac gacggtggtc
    61021 agtggaacga cgatgcagaa ctgaacttcg gcaacggcaa cttcaacgtt cgctctaccg
    61081 gctggggtgg ttacctggtt gtaggttaca acttctgatt atgaaaatgc cgggatttat
    61141 tcccggcatt tcagattgtt aacaacgtgc gctttgttca tgccggatgc ggcgtaaacg
    61201 ctttgtccga cccacaaaag cacgtaaatt caatatattg ctgaaataac gtagtcctga
    61261 taagcgtagc gcatcaggca attttacgtt ttaatcactt cttcttgttc atcatcgctt
    61321 tcaggtcagc gaatgggttg tacttcgctt caccgacatc tttctgcgca tcttcccctg
    61381 cgataacggt cgtaccatac tgatccgctt cggtatattt cgaatgctca tgatcgtggc
    61441 aatagagaca aagcaattcc cagttactgc catcttccgg gttattggta tggtcgtgat
    61501 caatgtggtg aacagtaagt tcacgcaggt tggaataaac aaactcgcgg gaacagcgac
    61561 cgcagaccca cggatatatt tttaatgctt tttcgcgata gccgctttct aaccgcgcat
    61621 agttttttgg gatgatagcc atgaagagtt acctggatga aatgtagggt tacgcgcagt
    61681 ttcccaagat gcggggattt tcgcaatctc catacatcaa aaaaaatccc gatcttctga
    61741 ccgggatttt caacatcaac gggaacttga ttacggcaga attgacggct gatccgcccc
    61801 ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa
    61861 cgcagatgcc acatagatgg aagatgcagt accgatggaa acaccgataa gcatggtcag
    61921 cgagaagcct tccagtaccg gaccaccgaa gaggtacagc atcaggataa ccatcaaggt
    61981 agtaccggat gtgatcaagg tacggtgcag cgtctgggtc aaggacacgt taaagatttc
    62041 gtaaggcgta ccgcgacgga tcttgcggaa gttttcacga atacggtccg ataccacgat
    62101 actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg tcaggtcaat
    62161 ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat
    62221 aaccacccct gccgccagtc gccactcaaa gcgaaaacct acgtacacaa ggatagacag
    62281 cagcgccgcc atcaacgcca tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc
    62341 gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa tcaccttcag
    62401 aacctggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca tacggaccat
    62461 gatgtcgtgg ctgctaccaa agttttgcag cattggctct tcaaaaccgg ctttttgcaa
    62521 tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga taaccgtacc
    62581 accggtgaaa tccagccccc agttaaagcc gcgcacgccc ataataacga tagcggcgat
    62641 taacagcaga ccagagatgc cgaaagccca gtagtcccag cgcataaagt catagacttt
    62701 acggccgtgg tttagttgtt caacagtata ttcctgtgcc acatcgcact cctcagattg
    62761 acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc gtaccgacaa
    62821 tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac cctttaattg
    62881 ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg ttcgcatcga
    62941 agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca gtacgtccgt
    63001 tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc gccaccgcaa
    63061 gggttaagac gatacccgcg atacctggca tactcagcgt tgcgcctggc aacagcgaca
    63121 taatgccgac gattaagatc aagttggcaa tcagagcact ggtcgcaatc agaccaaact
    63181 tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg caagcctcca
    63241 gcccctgttc aatgttctgc atacccaggg ttgggccaat ggtacgttct tcaacaatct
    63301 gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg gcttcgttcg
    63361 ggttgttgat gccggtgata cggaagctgt tacccagacg agactggatg ttggcgatgt
    63421 taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc ttaccgctgt
    63481 ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtcctta gtgaagttag
    63541 acatgatgtt accaccagcg ctatcgagcg agatgttaac ctgcggttgg ttgtattcgt
    63601 cctggctgga agtggagtcg gtgatatggt caccggtcag aattacgcgt ttgtacagca
    63661 caactggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg cgaccggatg
    63721 ccgcagcggc ctggtcaacg ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac
    63781 ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca acgatacggt
    63841 cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca cggttacgca
    63901 ggatattgat gttctgctgc accgcatatt cacgcgcttc actcagacga gcatcgctca
    63961 ttaccgcacg cagctggttg ctgccctggc tactaatcac caggtccgga tggcgcttgc
    64021 tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtaatg ctcaggccgt
    64081 agttgttttc tttacgaacg gtggtatacg ggatgccctt ttcacgcaga tcgctgcgca
    64141 ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc acttccatca
    64201 ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagcg tgaatagctg
    64261 ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtatttg tcacccataa
    64321 cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa
    64381 tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt aacgttttct
    64441 ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg atctgaacag
    64501 ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca atcaccacga
    64561 tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc ttagggaaaa
    64621 attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg aagtcacgtt
    64681 taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt tccgctactt
    64741 tggttacgcg accaaccagg ccaccgttcg tcagaacttc atcacctttg gcaatggagt
    64801 ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc atgaaataga
    64861 aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct tgcgccggtg
    64921 cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc tcattattaa
    64981 tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa atcagttacg
    65041 aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag
    65101 taacgaaggt tatgaatggt gttgagtcgc gcgcctaata tttcgttgca acggtcaaga
    65161 tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc aggatcgagt
    65221 gggccagtat cgctcttata cttcgcattg cggattttca ccacgccatc ggtcacgaac
    65281 aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc gataccacga
    65341 cgtacgcctt caaccaggtc ttctggttta ccaacgccca tcaggtaacg cggtttgtct
    65401 gccggaattt gcgggcatac atgctccaga atgcggtgca tatctgcttt cggctcaccc
    65461 acagccagac cgccgacagc gtagccatca aaaccgatat ctaccagacc tttaacagaa
    65521 atatcacgta aatcttcgta aacgctgccc tgaatgatac caaacagcgc atttttgttt
    65581 ccgagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat ctccatggag
    65641 cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat catgacgata
    65701 tccgaaccaa gatcgtactg aatctccatt gatttttcag gatcgaggaa aatcggatcg
    65761 ccgttgatcg ggttacggaa gtgcacgccc tgttcggtga ttttacgaat atcgccaagg
    65821 ctgaagacct ggaagccgcc ggagtcggtg aggatcggcc ccttccactg cataaaatcg
    65881 tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag gtggaaggtg
    65941 ttgccgagga taatttgcgc gccagtggct tcaacttctt ccggcgtcat cccttttacg
    66001 gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccaca atcaaagacc
    66061 aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat tttttctcca
    66121 ctacgtcaga aaaacagtcc aacgttttaa accagcgccg cggaattact ccccgacgcg
    66181 ctcattaatt gcctgcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg
    66241 atatttctct tctaccgctg ctttataggc gttcatggtg tgttgataac cggcaaaggc
    66301 cgaaaccagc ataatcagcg tcgattccgg caggtggaag tttgtcacca gcgcatcgac
    66361 cactttgtac tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat
    66421 aagatcgttt ttcgccgcct gagccgcgct ttccagcgaa cgtactgagg tggtgccaac
    66481 cgcaatcacc cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg
    66541 cacttcagcg tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg
    66601 gaaggtgccc gcaccaacgt gcaacgtcac aaacgccatc tccacgcctt tggcgcgtaa
    66661 tttttccagc aaaggttcgt caaaatgcag acctgcggtc ggggctgcaa ccgcgcccgg
    66721 tttttcgcta taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacggtcgat
    66781 atacggcggc agcggcatat ggccgatgct gttgagaata tccagcaccg agcgttcatc
    66841 attaaattcg acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt taatactttc
    66901 gtcatcgccc agcagcagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc
    66961 aagaatgcgt ttgtcgtcga gcatccgttc aaccagcact tcaatcttgc cgccgctggc
    67021 tttacgccca aacaggcgcg ccgggatcac gcgggtatta ttaaaaacca gaagatcgcc
    67081 ggggttgagc ttatcaagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc
    67141 gtccagcgac agtaaacgac agctactgcg ttcaggcatg ggatagtggg caatcaggga
    67201 ttcgggcaat tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag
    67261 aggcgggtag tctagtgccg gggcgctctc cctgcaacct ttaccccctt ttggatagaa
    67321 tataatcgat gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt
    67381 ccggcaattt actggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg
    67441 tcgtggctgg cattcatatg catcgacgta tcgacgtatt aaccgacaat ctgccggaag
    67501 tccgcgaagc acgggaatgg tttcgtagtg aaactcgccg cgttgcgcct attacgctgg
    67561 atgtcatgtg ggatcacttt ctttcccgcc actggtcgca gctgtcgccg gactttccgc
    67621 tacaggaatt tgtctgttat gcccgcgagc aagtgatgac gattttgccg gactcaccgc
    67681 cacgttttat caatctgaac aattacttgt ggtcagagca gtggctggtg cgctatcgcg
    67741 atatggattt catccagaac gtgttaaacg gcatggcaag ccgccgccca cgtctggatg
    67801 ccctgcgtga ctcctggtac gatttagacg ctcattatga cgccctcgaa acccgcttct
    67861 ggcagtttta tccgcggatg atggcgcagg cgtcacacaa ggcgttataa aacgttagtt
    67921 catccatacc gtagccgaaa tggcaggcaa agccagaatc ccgtcagtca gttgcccatg
    67981 cccttctttg cattgccatt gcacggcatt gagcaacggt gacgcgggta gcaccacttc
    68041 acaagcttcg ccacggttga ttgccaccag tacacgctgc tgattcagca cgcggacaaa
    68101 taccaccacg ttatcttccg catacagcac ctgacagccg ccatgacgta gcgcctgact
    68161 tttcttacgc agcgcaatca ttcgctggta cagcgcgaat aacgccgtat cctgcttttc
    68221 cacctgccag gggaacggtt tacggcaaaa cggatcgttt ttgccatcca gtcctacttc
    68281 atcaccgtaa taaatgcacg gtacaccagg ccaggtgaac agccagacca ccgccagcgg
    68341 caggcgcgca atatcccgac cgagcagcgt tttaaatcgc gcagtatcgt ggctgtcgag
    68401 ctgattaaac atacgtaatt gttgttgatg agaaagccct gcgcggtaat tatccatcca
    68461 ggccatacag gtttgggcat caatttgctg cggatcgtaa gagatatcgg tattggcaag
    68521 aaatccccac aacgggaatg tgaagccacg atagttcatg gcggcatctt ccacatcggc
    68581 ctgtaaccat tgccgtgcat cgccaaaatg ttcgccgaca atatacgctt ccggctgggt
    68641 ttctttcgcc gcttcggtga tcccagcaac gtgctgcata ttattgcgcg ccccacccgc
    68701 ctcccccagc atatgcacca catccagccg ccagccgtcc atactccacg gcgctttcag
    68761 ccaatggcgg acaatactgt cttccccgcg ataaatttca ttcaccagac tttccgactg
    68821 ataatccagc ttcggcaagc tggcatagcc aagccagtcg agcgccgtgc catcatcact
    68881 aaacgagtac cagtcgcgcc agggcgattc agggttgtga caagctcccc ccgtgccacg
    68941 attgtgcctg tcaaaccagg catgggaatc gccactgtgg ttaaacacgc catccagcac
    69001 cagccgcatt cccagctgct gcgtattgtg gcgtaagcgc agcaacgccc catcaccgtc
    69061 aaactgcgga tcgacatggc gataatcctc ggtatcgtat ttatgtacgc tgggagcttt
    69121 aaacaccgga ttgagataca gcgccgtcac gccaagcttt ttcagatacg gcagtttttc
    69181 gcttatcccg tccagatcgc cgccatagaa cgttgatccg cccgcctgcg ccgtgaccgg
    69241 ttcatcccag tcacgcaaga tgatctcttg tccggctgca tgatggtaat agacatgatc
    69301 ctgttcagct tcacgaggaa gactacgcgc aaaacgatca gggaagatct gataaaaaat
    69361 ctgatccgca gcccattgtg ggccgatatc cggtacatcg acggcaaact gctccagtcg
    69421 tgccggcggc attcggctga agccctgcgg tgtaaaccaa cgctggcgat cgtgccacag
    69481 cagtttgaaa ctgtaacgcc gccggggttg tccgctggag agatcaatcg ccgcacgcca
    69541 tgcggtgacg ccaggctgct gctgactgcg ctgcttatgc atcgatactg acatttcttc
    69601 gttatcgtgt tctgtacgca gcataatgcg ctgcggtggg tcttcgcccg tcagccacag
    69661 tgtaatgagc agttgatctt tgctttgttt aacaaatggg ggcaccggca ggtgccatgc
    69721 atttaacatc ataattcccc tgtgatgaaa atctgatcac cttgccatag cctaatgcag
    69781 gatcactcat catctgaagg atatttgggg gaggaggacg ggggcggata actttcggaa
    69841 tagccggatc aggatttcct cctgaaccgg cttacagaac gcaagtatta ttggttttca
    69901 gccagctgac gatcgtggcg gcgtttaaac atccagccaa tcaacagcac aacaatccac
    69961 gcgaaaccga catacagcga gatacgcgta tccgggtgat aaccaatcaa cccgataata
    70021 aagagcagga aaatcagccg gccgatggtc gttgctaccc cacccggcac tttaaatttc
    70081 agcgccttaa cttcttctgg cggcaaacgg cgacggaagg caatttgcga cagcaggatc
    70141 ataatccaca cccacaccgt ggcgaaggtt gccagcgaag cgatcaccag gaagacgttt
    70201 tccggcatga tgtagttcag atacaccgca aacagcagcg cggtagtcat caccagcacc
    70261 gtaacccacg gaataccgcg acgcgacgtt ttgctgaaaa ttttcggcgc gctgccctgc
    70321 tctgccatac cgtggagcat acggcctacg ccaaatacgt cactgttaat tgccgacagc
    70381 gaagcagtca gcacaacaaa gttaagaatg ctggcggcaa aggtaatgcc catatgctgg
    70441 aacgtcagca cgaacgggct accggcagtg ccaacctgat tccacgggta gatagacata
    70501 atgacgaaca gcgtaccgac gtagaacacc agaatacgca tcggcacgga gttaatcgca
    70561 cgcggtatcg atttctcagg atctttcgct tcaccggcgg taatcccgat aatttcgatc
    70621 ccaccgtaag caaacatcac catttgcaac gacatcacca tcccaagcca gccgttactg
    70681 aagaagccgc cgttgctcca caggttatga ataccggtcg gttgcccgcc gttgccaatc
    70741 ccccagatga tgatgccgaa accggcgaca atcatgatga tgatggtggc gactttaaag
    70801 aacgagaacc agaattccag ctcaccgaag accttcacgc tcatcaggtt tacggcgcaa
    70861 atgatcagca ccacgctcag tacccaaatc cagtgcggca ccgtcgggaa ccagacaccc
    70921 atatagatac caaaagcggt cacatcggcg atggcgacaa taaggatttc aaagcaatag
    70981 gtccagccgg taatgtaacc tgccagcggg ccgaggtttt cctgcgcata acgcgagaaa
    71041 gagctggctg ccgggttatg taccgacatt tcccccagcg cacgcataat gatatacgcc
    71101 gcgataccac cgataatata ggccaacaac acgctcggac cggccatttt gatggcgtct
    71161 gccgaaccgt aaaacagccc ggtgccaatt gctgaaccca gtgccataaa gcgtatgtgg
    71221 cggtacttag cccacgcttt agcttgttct tactttccat caattcctaa cccatcaaca
    71281 caataaaaaa ccacggggca tcgcagcccc gtggttaaaa caaatgttca gtgatttagt
    71341 gagcgctgga ggtcacctga cgacctgccg cgcgatccca gataatggcc agaaccacca
    71401 tcaccactgt tggcattaac cacgccagac cttgttcggc cagcggtaaa cgctgcgccc
    71461 aggacggtaa gatatcgctg aatgcagatg ctttgatccc gtcgagaata ccaaaaagca
    71521 ggctgataaa catcggcgga gcaatcacgc gggacgaatt atgccaccat gagcgtgtaa
    71581 aacttaatac aaccagtgcg atacacggcg gataaatagc ggtcagcacc ggtacggaga
    71641 tctggatcag ctggcttaag ccgaggttag aaaccaccat cgagaagccg ccgaggataa
    71701 acaccagcgt acgataagag agcggtacgt actgggcaaa gaattctgca caagcacagg
    71761 tcaggccaac tgccgttacc aggcaggcga tgaagattaa cgccgccagc aggaagctac
    71821 cgccgccgcc aaaggtgtgc tgaacgtaag catgcagaat agcagcgccg tttgcagact
    71881 gatcgaccag cgacgcgctg tctgaaccca gacggaacag cgccaggtac agcagagtca
    71941 gaccaacacc cgccatcagg ccagcccaga cggtataacg ggtcagcaga cgcgcttcgg
    72001 taacgccacg agaacgcgcc gcgttaacaa taacgatacc aaacaccatt gcgcccagcg
    72061 tatccatggt cagatagccg ttaacgaagc cgttagaaaa cgcagcgttt tgataagcct
    72121 cagtcgccgt gctgatagaa cccgccggcc agacaatcgc ggcaacagac aggatgacca
    72181 gcgcgataat tttcagcggc gcaaggaagt tgcccacggt atccagcagc ttgcccggat
    72241 agagcgaaac cagaataacg atagcgaaat agaccaggct gtagataaac agcggcagcg
    72301 cggaatcacc cgtcagcggc gcaatcccca cttcaaagga aacggtagct gtacgcggcg
    72361 tagcgaaaag cggccccacc gccaggtaac aaaccgttgc cagcagtacg ccagcgactt
    72421 taccgattgg cgtgctgagg ctgtcaacac cgccgccaac ttttgccagc gccactaccg
    72481 ttaataccgg caggccaacg gcagtaatga ggaagccgaa tgccgcagtc cagacgtgtt
    72541 cgcctgcctg taagccgacc attggaggga aaataatgtt acctgcgccg acgaacaacg
    72601 caaatgtcat aaagcccaga gcgatgatat cgcgcgatct taattgatgg gtcataaatc
    72661 atactgcctg tggatgtggt gttgtaacgt taaatttttg tgccaatccc tggggatgat
    72721 acagcggtat atttaaacaa ttttagcggg aaatactccc aatcaggcgc ggcgaagaat
    72781 agtttttcga tttaccgcgt taatgcagtc agtatgacag cacctgaagc gcaatttaaa
    72841 cgcttataac gttttaaagc aaggagggat ctaaaaacca gaggtatata gcgataagaa
    72901 atcaccaacc agtataaaac actatttccg tgaaaaagac atggctaatc atgcgaacaa
    72961 aaaatcacca accgttgtaa aatctaacgc ccgtattttg actgcaaaga aaataagcca
    73021 gcatcggctg gcttatggca ataaaagatg acaaaggcgg attaatcgct gtttttggca
    73081 attaaacgtt ccgggataac aaaactgaaa cgtgttcctt ttcctactgt actctcaata
    73141 ttcaggcgac tttcgtgatg attcaccgca tgtttcacga tcgctaaccc taatccgcta
    73201 ccgccggttt gccgggaacg cgctttatca acgcgataaa aacgctcggt caggcgcgga
    73261 atatgctccg gtgcaatgcc cggtccgtta tcttcaacgc taaattcggc accgtgcggc
    73321 actcgcagcc agcgtacggt gatatgcgtg ccttccggcg tatgattcac ggcgttatag
    73381 accaggttcg aaatcgcact gcgtagctga tcttcgttgc cagacacctt gaggccgtta
    73441 tctatctcaa aggtaaatgt ctgttttttc tgactcagag tctgagcctc gcgctcaaca
    73501 acgcgcagca tcatcggcac atcaaccttt tcattgagca attgcgtcgg cgcggcttca
    73561 attttcgaca gcgttagcaa ctgcttcacc agcccttcca tccgctgggt ctgctcgcgc
    73621 atggtgtgca acgctttttc gcgtaccgcg ccttccagcg gctgctcatc catcatctcc
    73681 aggtaaccct gtaacacggt caatggcgta cgtaactcat ggctcacgtt ggcaaaaaag
    73741 ttacgccgcg ccccttccag ttgatgcatt tgcgtgacat cacgcgccac catcagcaac
    73801 tgtttgtggg tataaggcat gacgcgaatt tccagatgcc gcccggtgtt gagcaccaga
    73861 ttgagcgggc gagaaaaatc acgcgttttc agatattgcg taaactccgg gtaacgcagc
    73921 aggttaagga tgttctgccc gttatcttcc ggccagcgca aaccaagaat ttgttgcgcc
    73981 agaccgttac accagaaaat accgccctct tccgtggtca gcaccactgc gtcgggcagc
    74041 gactccgcgc cgctacgaaa gcgtttaatc agattgccca gttcacggcg gcgtttttta
    74101 tttcgcagct gcatctggtg taagccgtat agtagcggtt cccagctacc acgccccggt
    74161 ggcggggtca tactacgatc cacccacagc caccatgaaa ggcgcaataa attccagaaa
    74221 tgccagataa gcagtcccgt taccgatgcc agcaaaaacc agggcaggta accaaaaaat
    74281 gcacccagga tgaaagccgg gaggcagcaa agtagcagct ccagcaccag ccttttccac
    74341 gacagccgtt ccagcacgta agatactcca gttaagaaat cataagccct gctctgcgtc
    74401 cgatgagcaa ggcgttaaaa gcgggttgaa aaacgatatc ctgtaccgcg cacggtctgc
    74461 accatgcggt catgcccgcc gggctccagt gctttacgca ggcgacgaat gtggacatcg
    74521 accgtgcggt cttccacata cacgttagtt ccccagacgt ggtttaacag ctgctcgcgg
    74581 ctgtacacgc gctcaggatg cgtcataaaa aagtgcagca gtttaaattc tgtcggcccc
    74641 atctccagcg gctcttcgcc cgccatcact cggtgagatg tcgggtcgag acttaatccc
    74701 tgcatctcaa tcacctcttc caccgccatt ggcgaaatac ggcgcattac cgctttgatt
    74761 cgcgccacca gctccttcgg cgaaaacggc ttggtgatat agtcatccgc gccggtttca
    74821 aggccgcgca cgcgatcttc ttcttcccct ctggcggtca acatcaccac tggaatatcc
    74881 cgggtcatcg actcgcgctt gaggtgtttg atgaactgga taccggagcc gccaggtaac
    74941 atccagtcaa ggagaattaa atccggccag ggttcattca gttgattcac agcactgtca
    75001 taatcttccg cttcgaccgg ctgaaagcca ttttgttcga gcacgaagca gaccatttcg
    75061 cgaattggag cttcatcttc tacgaccaga atacgtctcg tcatgatttg ccctgttgta
    75121 ataaataggt tgcgatcatt aatgcgacgt cattatgcgt cagatttatg acagatttat
    75181 gaaaagctcg tcgcacatat cttcaggtta ttgatttccg tggcgcagaa aaaagcaaat
    75241 ggcacatctg tttgggtata atcgcgccca tgctttttcg ccagggaacc gttatgcgca
    75301 tccttcacac ctcagactgg catctcggcc agaacttcta cagtaaaagc cgcgaagctg
    75361 aacatcaggc ttttcttgac tggctgctgg agacagcaca aacccatcag gtggatgcga
    75421 ttattgttgc cggtgatgtt ttcgataccg gctcgccgcc cagttacgcc cgcacgttat
    75481 acaaccgttt tgttgtcaat ttacagcaaa ctggctgtca tctggtggta ctggcaggaa
    75541 accatgactc ggtcgccacg ctgaatgaat cgcgcgatat catggcgttc ctcaatacta
    75601 ccgtggtcgc cagcgccgga catgcgccgc aaatcttgcc tcgtcgcgac gggacgccag
    75661 gcgcagtgct gtgccccatt ccgtttttac gtccacgtga cattattacc agccaggcgg
    75721 ggcttaacgg tattgaaaaa cagcagcatt tactggcagc gattaccgat tattaccaac
    75781 aacactatgc cgatgcctgc aaactgcgcg gcgatcagcc tctgcccatc atcgccacgg
    75841 gacatttaac gaccgtgggg gccagtaaaa gtgacgccgt gcgtgacatt tatattggca
    75901 cgctggacgc gtttccggca caaaactttc caccagccga ctacatcgcg ctcgggcata
    75961 ttcaccgcgc acagattatt ggcggcatgg aacatgttcg ctattgcggc tcccccattc
    76021 cactgagttt tgatgaatgc ggtaagagta aatatgtcca tctggtgaca ttttcaaacg
    76081 gcaaattaga gagcgtggaa aacctgaacg taccggtaac gcaacccatg gcagtgctga
    76141 aaggcgatct ggcgtcgatt accgcacagc tggaacagtg gcgcgatgta tcgcaggagc
    76201 cacctgtctg gctggatatc gaaatcacta ctgatgagta tctgcatgat attcagcgca
    76261 aaatccaggc attaaccgaa tcattgcctg tcgaagtatt gctggtacgt cggagtcgtg
    76321 aacagcgcga gcgtgtgtta gccagccaac agcgtgaaac cctcagcgaa ctcagcgtcg
    76381 aagaggtgtt caatcgccgt ctggcactgg aagaactgga tgaatcgcag cagcaacgtc
    76441 tgcagcatct tttcaccacg acgttgcata ccctcgccgg agaacacgaa gcatgaaaat
    76501 tctcagcctg cgcctgaaaa acctgaactc attaaaaggc gaatggaaga ttgatttcac
    76561 ccgcgagccg ttcgccagca acgggctgtt tgctattacc ggcccaacag gtgcggggaa
    76621 aaccaccctg ctggacgcca tttgtctggc gctgtatcat gaaactccgc gtctctctaa
    76681 cgtttcacaa tcgcaaaatg atctcatgac ccgcgatacc gccgaatgtc tggcggaggt
    76741 ggagtttgaa gtgaaaggtg aagcgtaccg tgcattctgg agccagaatc gggcgcgtaa
    76801 ccaacccgac ggtaatttgc aggtgccacg cgtagagctg gcgcgctgcg ccgacggcaa
    76861 aattctcgcc gacaaagtga aagataagct ggaactgaca gcgacgttaa ccgggctgga
    76921 ttacgggcgc ttcacccgtt cgatgctgct ttcgcagggg caatttgctg ccttcctgaa
    76981 tgccaaaccc aaagaacgcg cggaattgct cgaggagtta accggcactg aaatctacgg
    77041 gcaaatctcg gcgatggttt ttgagcagca caaatcggcc cgcacagagc tggagaagct
    77101 gcaagcgcag gccagcggcg tcgcgttgct cacgccggaa caagtgcaat cgctgacagc
    77161 gagtttgcag gtacttactg acgaagaaaa acagttactt accgcgcagc agcaagaaca
    77221 acaatcgcta aactggttaa cgcgtcagga cgaattgcag caagaagcca gccgccgtca
    77281 gcaggccttg caacaggtgt tagccgaaga agaaaaagcg caacctcaac tggcggcgct
    77341 tagtctggca caaccggcac gaaatcttcg tccacactgg gaacgcatcg cagaacacag
    77401 cgcggcgctg gcgcatattc gccagcggat tgaagaagta aatactcgct tacagagcac
    77461 aatggcgctt cgcgcgagca ttcgccacca cgcggcgaag cagtcagcag aattacagca
    77521 gcagcaacaa agcctgaata cctggttaca ggaacacgac cgcttccgtc agtggaacaa
    77581 cgaactggcg ggttggcgtg cgcagttctc ccaacaaacc agcgatcgcg agcatctgcg
    77641 gcaatggcag caacagttaa cccatgctga gcaaaaactt aatgcgcttg cggcgatcac
    77701 gttgacgtta accgccgatg aagttgctac cgccctggcg caacatgctg agcaacgccc
    77761 actgcgtcag cgcctggtcg cgctgcatgg gcagattgtt ccccaacaaa aacgtctggc
    77821 gcagttaatg gtcactatcc agaatgtcac tctggaacag acgcaacgta atgtcgcact
    77881 taacgaaatg cgccagcgtt ataaagaaaa gacgcagcaa cttgccgatg tgaaaaccat
    77941 ttgcgagcag gaagcgcgca tcaaaacgct ggaagcccag cgtgcacagt tacaggcggg
    78001 tcagccttgt ccactttgtg gttccaccag ccatccggcg gtcgaggcgt atcaggcgct
    78061 ggagcctggc gttaatcagt ctcgattact ggcgctggaa aacgaagtta aaaagctcgg
    78121 cgaagaaggt gcggcgctgc gcgggcaact ggatgcatta acgaagcagc ttcagcgcga
    78181 tgaaaacgaa gcgcaaagcc tccgacaaga tgagcaagca cttactcaac aatggcaagc
    78241 cgtcacggcc agcctcaata tcaccttgca gccacaggac gatattcaac cgtggcttga
    78301 tgcacaagat gagcacgaac gccagctgcg gttactcagc caacgacatg aattacaagg
    78361 gcagattgcc gcgcataatc agcaaattat ccagtatcaa cagcaaattg aacaacgcca
    78421 gcagcagctc ttaacggcat tggcgggtta tgcactgaca ttgccacagg aagatgaaga
    78481 agagagctgg ttggcgacac gtcagcaaga agcgcagagc tggcagcaac gccagaacga
    78541 attaaccgcg ctgcaaaacc gtattcagca gctgacgccg attctggaaa cgttgccgca
    78601 aagtgatgat ctcccgcaca gcgaagaaac tgtggcgctg gataactggc ggcaggttca
    78661 tgaacaatgt ctcgcattac acagccagca gcagacatta cagcaacagg atgttctggc
    78721 ggcgcaaagt ctgcaaaaag cccaggcgca gtttgacacc gcgctacagg ccagcgtctt
    78781 tgacgatcag caggcgttcc ttgcggcgct aatggatgaa caaacactaa cgcagctgga
    78841 acagctcaag cagaatctgg aaaaccagcg ccgtcaggcg caaacgctgg tcactcagac
    78901 agcagaaacg ctggcacagc atcaacaaca ccgacctgac gggttggctc tcactgtgac
    78961 ggtggagcag attcagcaag agttagcgca aactcaccaa aagttgcgtg aaaacacgac
    79021 gagtcaaggc gagattcgcc agcagctgaa gcaggatgca gataaccgtc agcaacaaca
    79081 aaccttaatg cagcaaattg ctcaaatgac gcagcaggtt gaggactggg gatatctgaa
    79141 ttcgctaata ggttccaaag agggcgataa attccgcaag tttgcccagg ggctgacgct
    79201 ggataattta gtccatctcg ctaatcagca acttacccgg ctgcacgggc gctatctgtt
    79261 acagcgcaaa gccagcgaga cgctggaagt cgaggttgtt gatacctggc aggcagatgc
    79321 ggtacgcgat acccgtaccc tttccggcgg cgaaagtttc ctcgttagtc tggcgctggc
    79381 gctggcgctt tcggatctgg tcagccataa aacacgtatt gactcgctgt tccttgatga
    79441 aggttttggc acgctggata gcgaaacgct ggataccgcc cttgatgcgc tggatgccct
    79501 gaacgccagt ggcaaaacca tcggtgtgat tagccacgta gaagcgatga aagagcgtat
    79561 tccggtgcag atcaaagtga aaaagatcaa cggcctaggc tacagcaaac tggaaagtac
    79621 gtttgcagtg aaataactat tcagcaggat aatgaataca gaggggcgaa ttatctcttg
    79681 gccttgctgg tcgttatcct gcaagctatc actttattgg ctacggtgat tggtagccgt
    79741 tctggtagtt gtgatggtgg tatgaaaaaa gtcattttat ctttggctct gggcacgttt
    79801 ggtttgggga tggccgaatt tggcattatg ggcgtgctca cggagctggc gcataacgta
    79861 ggaatttcga ttcctgctgc cgggcatatg atctcgtatt atgcgctggg agtggtggtc
    79921 ggtgcgccaa tcatcgcact cttttccagc cgctactcac tcaaacatat cttgttgttt
    79981 ctggtggcgt tgtgcgtcat tggcaacgcc atgttcacgc tctcttcgtc ttacctgatg
    80041 cttgccattg gtcgcctggt ttccggcttt ccgcatggcg cattttttgg cgtcggcgcg
    80101 atcgtgttat caaaaattat caaacccgga aaagtcaccg ccgccgtggc ggggatggtt
    80161 tccgggatga cagtcgccaa tttgctgggc attccgctgg gaacgtattt aagtcaggaa
    80221 tttagctggc gttacacctt tttattgatc gctgttttta atattgcggt gatggcatcg
    80281 gtctattttt gggtgccgga tattcgcgac gaggcgaaag gaaagctgcg cgaacaattt
    80341 cactttttac gcagcccggc cccgtggtta attttcgccg ccacgatgtt tggcaacgca
    80401 ggtgtgtttg cctggttcag ctacgtaaag ccatacatga tgtttatttc cggtttttcg
    80461 gaaacggcga tgacctttat tatgatgtta gttgggctag ggatggtgtt gggaaatatg
    80521 ctaagtggca ggatttcagg acgttattca ccactgcgca ttgcagcagt gactgacttt
    80581 ataattgtac tggcactgct gatgctcttt ttctgctgcg gcatgaaaac aacgtcgctt
    80641 atttttgctt ttatttgttg cgcgggatta tttgcccttt cagcacctct gcaaatattg
    80701 ttactacaaa acgccaaagg cggagagtta ttaggtgccg caggtgggca aatagcgttt
    80761 aacctcggta gcgccgtcgg cgcatattgc ggaggtatga tgctgacgct ggggctggca
    80821 tataattacg tggcgctgcc tgccgccctg ctttcgtttg ctgcgatgtc atcgttgctg
    80881 ctgtatggtc gctataagcg ccagcaagcg gcggatagtc cggtgctggc gaaaccactg
    80941 gggtagatta ctgtcacggt gcgcgttgct tattgccgga tgcggcgtga acgccttatc
    81001 cgccctacgc ggttctggca cattttgtag gcctgataag acgcggcaag cgtcgcatca
    81061 ggcatgatgc gccaattgcc tacgtttttt actcttgtgg ccataaccac gcagcgccgc
    81121 gtacgccgct ggaatcaccg tgcttcgcct tacgcaccgg cgtttcacat tcgccgccga
    81181 agacaaattg tttaatcaac tgcggaaccg tttgatataa acggtctaca ttgctcatcc
    81241 cgccccccag gacaatcaca tccggatcga gaatattcac gacatgtgcc agcgattttg
    81301 ccagccgcag ctcgtagcga cgcaatgcca gttccgctac cggatcgctt tcttcaacca
    81361 ggctgataat ttcactgcct ttcagcgcat gtccgctcaa acgacgataa tccgtcgcga
    81421 atccagtgcc cgaaataaag gtttcaatac aaccttgttt accgcaataa caagggactt
    81481 cctcgcgata acgcagttcg tcttcgtcca tccacggtag cggattgtgt ccccactcac
    81541 ctgccgtgcc attgccgccg atatgcgccc gcccattgaa tgccacgccc gcgccgcatc
    81601 ccgtgccgat aatcacggca aataccgtct gcgctcccgc tgccgcgcca tctaccgctt
    81661 ctgaaaccgc cagacagttg gcgtcatttg ccagccgcac ttcccgctgc aaccgcgcgc
    81721 ttaagtcttt atcgaatggc tgaccgttga gccaggttga attggcattc ttcaccacac
    81781 cggtgtaggg cgaaatcgaa cctggaatgc ccatacctac cgttccgcgc tgccctgtcg
    81841 cctgctccgc catatcaacc aacgtggcga tcgtttcaat agtttgccgg taatcatcac
    81901 gcggcgtggg cagacgatgg cggtacaact gctcccctgc atcgcccagt gcaatcactt
    81961 cagttttggt gccgcctaaa tcgataccta tacgcacggt actctcctta tttttttcaa
    82021 tatcaatagc gtagagacgg acaaccggat tggcaatgca aggccgccga caattcgtta
    82081 tcatgcccgc taaatttaac gacaaggccg tggaaattat catgctgtgg ttcaaaaatt
    82141 taatggttta ccgtcttagc cgcgagattt cgctgcgtgc agaagagatg gaaaaacagc
    82201 tagcctcgat ggcatttacc ccatgcggca gccaggacat ggcgaagatg ggctgggttc
    82261 ctccgatggg atcgcacagc gatgcgttaa cgcacgttgc caatggtcaa attgttatct
    82321 gcgcgcgcaa agaagaaaaa atcctcccgt ctccggtgat taaacaggcg ctggaagcga
    82381 aaatcgccaa actggaagcg gaacaggcgc gtaagctgaa gaaaaccgaa aaagattcgc
    82441 tgaaagacga agtgctgcac tctctgctgc cgcgtgcttt cagccgtttt agccagacaa
    82501 tgatgtggat cgacacggtt aacggtttga ttatggtgga ctgcgccagt gccaaaaaag
    82561 cggaagatac gctggcatta ctgcgtaaaa gcctggggtc gttaccggtt gtaccattga
    82621 gcatggaaaa cccgattgaa ctgacgctga ccgaatgggt tcgctccggt agtgcggcac
    82681 agggcttcca gctgcttgat gaagccgagc tgaaatcgtt gctggaagat ggcggcgtga
    82741 tccgcgcgaa gaaacaagat ctgaccagcg aagagatcac caatcacatt gaagccggaa
    82801 aagtggtgac taaactggcg ctcgactggc agcagcgcat tcagtttgtg atgtgcgacg
    82861 atggttcgct caagcgtctg aagttctgcg acgagctgcg cgatcaaaac gaagatatcg
    82921 accgtgaaga tttcgcccag cgttttgatg ccgatttcat cctgatgact ggtgaactgg
    82981 cagcgttaat tcaaaacctg attgaaggat taggtggcga agcacaacgt taattgctga
    83041 ttttccttta atgccggatg cgacgcctgc cgcgtcttat ccggcgtacg aagccacacc
    83101 aggcatataa ttattcgcta cggcgggcaa taatttttag cgcagcaata ttatgcgttt
    83161 tatgctgtaa cttgctccat ggacgttgtg tcattgtttt tcctcaagcc agtgacgcgc
    83221 ttcactgcga attttccgca ttgccccggt tttcactaaa tcccgtaaca gcgaaagatc
    83281 gggttgttcg acaaataaga gtaatgatgg atcctctttt aaccacgtcg ttttcacatc
    83341 ggcagcgagc tgtggattat tcatagctag cgaccgatgc cgagactcgg tcaatgtcgt
    83401 taacaacgac gccggggtat gtgcgttgcg taatgccgtt tggcgcaaat gatcattgcg
    83461 actttgcagc aatgcacgcc accattcatc atctgattta tcttttatcg tagccagttc
    83521 cgcaacaata tctggagatc ttcactgata tttatatcaa ccttgatgaa ttatgaatat
    83581 accggaaatt gaaaaaatcg cagcaaagga aatattatta tgggttaaag agaggatatt
    83641 tcagaaaaat gccctcccgt tcaaggggaa ggcgaggaat tacagatagc ggcacagata
    83701 agaggtgggt tcggcaactt gcagatgaaa ctcactgtga ccgggaacat taaacaccga
    83761 accggcttca tacacctgcc agtcggtcgc gtcaggcagt aacacattca gcgcgccact
    83821 gattactgtc atctcttccg gctcagcggt gctgaaggtg tattcgcctt caaccataac
    83881 acccacgctg gcgcgaccag tgctgctgct ggaaaagccg attgatttca ctttgccgga
    83941 aaagtactca ttactttgaa gcataaactg gccctgtctg gtagttgaac atctacttca
    84001 ctataggggc cagaggcgct ggctgtcacg caaaattaca ccagtaattc cgcagccagc
    84061 cgtgcaatca atacgttaga cagcaagaca ggaacatcga gctgtttttg cagtaaatcg
    84121 cgatgacgtt ggtgaaatcc taaacaatcc agcatgatga catcagcccc ttttgccagt
    84181 aattctttcc cggcatcaat gattttttgt tctgaatcat gaatggggtt gcccaatgaa
    84241 aataccggcg atttctgcaa aatttgccat ttttgcgcct gaacggtcag catctcctca
    84301 accggaacga taacccccac ctgatgatct tcaacaatag aggaaaccag tggaggcaat
    84361 attcgcgacg gctcaagaaa gatcgtatta cgcgcagtca tactactaat gtttgctgta
    84421 ctcattaata aaatgacgtc ataaccctga ttatcgagca cttcaaccac accttgcagg
    84481 tctcgctcca ctttacgacg cgaaacatgg gccagctggt tgtcatttaa taatgtgaga
    84541 atggtgtctt cgcctgcttc tggcgcgtac tccgccatca cttcttcacg acttaacttc
    84601 cccagcaagc tatgatggga aatattatct tcgtcaatgt attccgtcag gagcggcaaa
    84661 acctcctgca taggtacaat gccgatggtc aggatcgcca acgacgcact cattgttcat
    84721 ccaccttact tttctttcgt cgttaattac cgggcaagtg tgaagcacca ctgctgacac
    84781 ttacttgtgc tacaaatgac aaaaagcgta gcagcagacg tgcacggcat aatgtaaaga
    84841 ttccaaatga ttccagtaat ggattgttat tgtttaatat tctaattatt agaaaaacat
    84901 gaattatgaa aaatgtgacg cagatccctt gtagtggcac tcagaatccc ttccgagcag
    84961 tctgatgcgg tcactctttg tcagggtcct catagcgttt tttcgcatcc atcgcttcct
    85021 gagccgtcgg atgttcactg atcaacgagt ctggtttagg atgatcggct ctgagttggt
    85081 accaggttac cgtctgtcct ggctttcctt tttcaatcgt cactatatat gcttcacgag
    85141 gataaggcgg tttcgttggc atcgttcttc cttatttcac gggatgaacg ttaagtatag
    85201 gcgctctaaa atcaacaatt gatcgtctgt gccagggcgc tacgaatttc agaaatcacc
    85261 tggctgggtt cgtttgttgc gtcgatgata atatgcgcaa cttcgcgata tagcgcatcg
    85321 cgttcttcca gcacttcctg aacttcttcg ctcagcggtt ttcccgttaa ggttggccgt
    85381 aaatcttctt ccggtgcagc ttgcagtcgg ttaaccagga ctgatactgg cgcacacaaa
    85441 taaaccacga tcccgttatt ttgcatgaag tgacgattaa attccgtcag aataatgccg
    85501 ccgcctgtag cgataacggt ggatggcgca gttaccgctt ccagcgccgc cgtttctctg
    85561 gcgcgaaatc ccgcccactc ttccctttcg acgatctccg cgaccgtcat attgagctgt
    85621 gattgcaacc actgatcggt atcgacaaac cgacggttaa gcgaatcggc aagggccatt
    85681 ccgaccgttg ttttaccaca gccccgaggc ccgatcagaa aaagaggttg tgtcatcgtg
    85741 ggttttcccc aataggtcgc aatgcggcga aagccggtgt catgagaata gcgatcatgc
    85801 catcaaacta gtacaatttc gattgtaaag aaaaaattcc acttaaagtg aaaatctcaa
    85861 tacatccctt actataccaa taaatattca agaatgaagt gtaaataata aattacattt
    85921 agccacgact acgttgcact tccagccacc acttctccag ctccgcggca aaggcctggc
    85981 ggtcacgttg tgaaaggcta tctggtccgc cggtctggat cccactggca cgtaaggtat
    86041 ccataaaatc gcgcatcgtc aggcgctcac gaatggtcgc tggcgtgtaa cgttcgccgc
    86101 gcggattaag cgccgctgcg cctttctcga tggcttcagc agccaaaggt atatctgcgg
    86161 tgatcaccaa atcgcccgct tcacactgcc ggacaatttc gttatcggca acgtcgaaac
    86221 ctgccgcgac gcgcagcgta cgaataaatc gcgatggcgg cacgcgtaaa ctctggtttg
    86281 ctaccagtac cagcggcatc tgcatacgtt ccgccgcgcg atacaaaatc tctttaatta
    86341 cattgggaca cgcgtcggca tccacccaaa ttgtcataaa gtcatccttt gttgggtaat
    86401 cctctattgt gtcgcgcttt tgccttccgg catagttctg tttatgcttc tgccagcgat
    86461 tatcaaaaca atgaatttca cggcaggagt gaggcaatgg aaaagaaaat cggttttatt
    86521 ggctgcggca atatgggaaa agccattctc ggcggtctga ttgccagcgg tcaggtgctt
    86581 ccagggcaaa tctgggtata caccccctcc ccggataaag tcgccgccct gcatgaccag
    86641 ttcggcatca acgccgcaga atcggcgcaa gaagtggcgc aaatcgccga catcattttt
    86701 gctgccgtta aacctggcat catgattaaa gtgcttagcg aaatcacctc cagcctgaat
    86761 aaagactctc tggtcgtttc tattgctgca ggtgtcacgc tcgaccagct tgcccgcgcg
    86821 ctgggccatg accggaaaat tatccgcgcc atgccgaaca ctcccgcgct ggttaatgcc
    86881 gggatgacct ccgtaacgcc aaacgcgctg gtaaccccag aagataccgc tgatgtgctg
    86941 aatattttcc gctgctttgg cgaagcggaa gtaattgctg agccgatgat ccacccggtg
    87001 gtcggtgtga gcggttcttc gccagcctac gtatttatgt ttatcgaagc gatggccgac
    87061 gccgccgtgc tgggcgggat gccacgcgcc caggcgtata aatttgccgc tcaggcggta
    87121 atgggttccg caaaaatggt gctggaaacg ggagaacatc cgggggcact gaaagatatg
    87181 gtctgctcac cgggaggcac caccattgaa gcggtacgcg tactggaaga gaaaggcttc
    87241 cgtgccgcag tgatcgaagc gatgacgaag tgtatggaaa aatcagaaaa actcagcaaa
    87301 tcctgatgac tttcgccgga cgtcaggccg ccacttcggt gcggttacgt ccggctttct
    87361 ttgctttgta aagcgccaaa tctgccgatt tcaaccactc acgatagtga ctcatttgtg
    87421 ggttcagcgg cgcaaccccc acactaatcc gtaaagttac ctgtggcgta ttcggcaagc
    87481 gtaacgtgtt tagcccttca cgcacccgta acatggcggt aatggcgctc tcagctggcg
    87541 taccggacat gattactgca aactcatcgc cgccaaaccg accaatcaca tcgctaccgc
    87601 gcagggtaat ttgtaactgt cgggtaagcg ccacaatcgc ttcatcgccc acatcatgac
    87661 cccaggtatc gttgatgctc ttgaaatggt cgatatcgat aatcagtaac gttgcatcgc
    87721 gattatgccg ccgacagtta tcaaattcat tgcgtaacat agtttcccaa tgacgtcggt
    87781 tatacacgcc cgtcatgccg tcgcgggtac tcatgacctg caacctgcgt ttatgttccg
    87841 ccagtttggt tgccgtctgg tagctgaccc agccaaacag cagaggataa acgacaataa
    87901 tgggaaggga gagccaccat tccagcggcg cactattgaa cgacacggta atgcccgtaa
    87961 gctcgagggt gacaaggcag gaaaccacca tcaacaccag acccgcgaca aacagacggg
    88021 ggccgcctgc ccccatcaaa ttcagacaca taatcatcaa catagcggtg gaaggcagca
    88081 cgtttacgcc cattacgcct acccacattc ccgctaatac tgcatcggtt tttaagttgt
    88141 aaatttcccg gctaagcgga tcgacggccc tgctcgctat ctgccaggct aaatgcggcc
    88201 agacgaacgc ccagccgacc aacaccagcc accaccagcc cggcggcggg tgcgaaacca
    88261 gcgttgaagc aatcggtaag aacatgccag ccaggccaac cgcacggggt agtctgacgc
    88321 gacgggcgaa gcgcagcccg gaccgctgat gttcgttttg aggagttaaa ggaggttcct
    88381 ccccatgcgc cgccgctttt ttgaaaaagt tttcatcatt cattattttt gggaacattc
    88441 agaaacaatt ttcccaaatt atagagacgg ggctaaaggc agaagtatga aatttcgggt
    88501 gagccacttt cgtagctcac ccgttagcac tatcaggtat caggcggctt tcttgaggca
    88561 ggcactcata aacttattac gatcatcacc tttcagagat tgttgcgttg cttgattatt
    88621 acattcgcgc atcttttgct gctgtggcgt caaacttttt tcaccaggcg cagacttgct
    88681 gttcttcagg caatcactca tgtaggtctt acgagcatcc cctttcaacg cctgcgccgt
    88741 cgcctgctga ttacaggatg tcatacgctg ttgttgtggg gttaaggttc tctcggcagc
    88801 gccgacggtg gttaaaaaaa ccagaccaaa aagcaaggta accagtaatg ttattttcat
    88861 agcaccatcc ctcttcatgt tttaaccatg agcgtatgcg cccgtgatct gccattaagt
    88921 ctggttgcta acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcggtttt
    88981 atttcagccc cagggcggct ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa
    89041 caacattggc ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt
    89101 gtgaatcctc ttcggagttc ccgtagctca tcaccatcac tgcgccatct ttggtattta
    89161 gcgcctgggt gaggcccgga gctttggtat ccggcgcgac aatctggctg gcgtgggcgt
    89221 gatcagcggt gactatgacc agcgtgttgc catccttttt agcgaattcc agcgcccgtt
    89281 gtacggcttc atcgagatcg accgtctcgc caatttgccc acaaggattc gcagcgtgat
    89341 cctgtttatc gattgacgca ccttcaactt gcaggaaaaa tcctttctca tttttactca
    89401 acaattcaat ggctttgtcg gtcatctgcg ccagggttgg tacactgtca ttacgttgcg
    89461 gattaggcgt acaggtgact gcgggcttat cgatattgcc gtggtacgtt gctttcggtc
    89521 ctagccagcg cactggcata ttgccgtcag caaacagtcc aagcagaggt ttttgctgat
    89581 tcgcttccgt caccgaattc agtgaggcag catcgctgac caactgataa ccacgcgcct
    89641 gtgcctgttc acgcagcgtt tttccctgcc attcaccggc ggttgccgtt tcagcaaagg
    89701 tttttgcgcc accgccaagc gtaacgtcgg cacgagcgtt aagcagctgt tcggtaatcg
    89761 atccttttcc gcctttttcc agagcgttac ccggacattt ttcactggtc gcgctcggac
    89821 cgtagcattt gcgcgaggtc acatgcgcca ccagcgcagc gggcgtggca tcctgcaact
    89881 ctgcggtaga aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg
    89941 ggtgatcttt ttcgtgaata tcgacgccca gcgcgccgtt ataggttttg acaccggttg
    90001 accaggcggt tgctgatgca gccgaatcgg tgacgtagtc cggtttgccg gtttttttat
    90061 tcagcgcata gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc
    90121 cgcccgcacc ttcggcataa ttacgtgcgg ctgtaatttc cgaatccccc atcccatcgc
    90181 caatcagcaa aataatattt tttgcaggtt tatcgctaag agaatcacgc agagcggcag
    90241 tctgatcacc cgttaaacgg cgagcaccgc cggatgcagt aatatcgccc tgagcagccc
    90301 ggttttccag aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg
    90361 ataagagtgc cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat
    90421 taaaaaataa aaacaaagcg actataagtc tcggccgtga caactttatg acagctgttg
    90481 aaaagattaa ctttttactg acgaggatgc ttcaataact tctttacgta atcgcgcagc
    90541 agctccgtat cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct
    90601 acctgatcaa tcaagcgctg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg
    90661 ataatctcca aagcttctac ctgcgcacac agttctttcg actcttcttc tttttgggca
    90721 agcttaaata acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat
    90781 ggagaagtta acattatgtt tcacaacttc atagtgtctg gtagtattat ttttatgaca
    90841 aaggaaatac tttgcgcaaa tattttttgt ttgattacca gcgaaacgtt tctcttattt
    90901 aaataat
//
LOCUS       DAZFKM010000003        87178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFKM010000003 DAZFKM010000000
VERSION     DAZFKM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            95..538
                     /gene="cmtB"
                     /locus_tag="ACHKAY_000203"
     CDS             95..538
                     /gene="cmtB"
                     /locus_tag="ACHKAY_000203"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO5698947.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            566..1954
                     /gene="cmtA"
                     /locus_tag="ACHKAY_000204"
     CDS             566..1954
                     /gene="cmtA"
                     /locus_tag="ACHKAY_000204"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO5698948.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1969..3246
                     /gene="yggP"
                     /locus_tag="ACHKAY_000205"
     CDS             1969..3246
                     /gene="yggP"
                     /locus_tag="ACHKAY_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5698949.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3243..4208
                     /gene="glpX"
                     /locus_tag="ACHKAY_000206"
     CDS             3243..4208
                     /gene="glpX"
                     /locus_tag="ACHKAY_000206"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO5698950.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4230..4739
                     /gene="fumE"
                     /locus_tag="ACHKAY_000207"
     CDS             4230..4739
                     /gene="fumE"
                     /locus_tag="ACHKAY_000207"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO5698951.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4736..5449
                     /locus_tag="ACHKAY_000208"
     CDS             4736..5449
                     /locus_tag="ACHKAY_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO5698952.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5421..6098)
                     /locus_tag="ACHKAY_000209"
     CDS             complement(5421..6098)
                     /locus_tag="ACHKAY_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5698953.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6092..6769)
                     /locus_tag="ACHKAY_000210"
     CDS             complement(6092..6769)
                     /locus_tag="ACHKAY_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5698954.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6757..7464)
                     /locus_tag="ACHKAY_000211"
     CDS             complement(6757..7464)
                     /locus_tag="ACHKAY_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO5698955.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7465..8043)
                     /locus_tag="ACHKAY_000212"
     CDS             complement(7465..8043)
                     /locus_tag="ACHKAY_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO5698956.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8066..8497)
                     /locus_tag="ACHKAY_000213"
     CDS             complement(8066..8497)
                     /locus_tag="ACHKAY_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO5698957.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8869..9888
                     /gene="epd"
                     /locus_tag="ACHKAY_000214"
     CDS             8869..9888
                     /gene="epd"
                     /locus_tag="ACHKAY_000214"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO5698958.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9938..11101
                     /gene="pgk"
                     /locus_tag="ACHKAY_000215"
     CDS             9938..11101
                     /gene="pgk"
                     /locus_tag="ACHKAY_000215"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO5698959.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11316..12395
                     /gene="fbaA"
                     /locus_tag="ACHKAY_000216"
     CDS             11316..12395
                     /gene="fbaA"
                     /locus_tag="ACHKAY_000216"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO5698960.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12459..12557)
                     /locus_tag="ACHKAY_000217"
                     /pseudo
     CDS             complement(12459..12557)
                     /locus_tag="ACHKAY_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12753..13613
                     /gene="mscS"
                     /locus_tag="ACHKAY_000218"
     CDS             12753..13613
                     /gene="mscS"
                     /locus_tag="ACHKAY_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO5698961.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13752..14387
                     /gene="argO"
                     /locus_tag="ACHKAY_000219"
     CDS             13752..14387
                     /gene="argO"
                     /locus_tag="ACHKAY_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO5698962.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14480..15220
                     /gene="yggE"
                     /locus_tag="ACHKAY_000220"
     CDS             14480..15220
                     /gene="yggE"
                     /locus_tag="ACHKAY_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO5698963.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15387..16283
                     /gene="ygfI"
                     /locus_tag="ACHKAY_000221"
     CDS             15387..16283
                     /gene="ygfI"
                     /locus_tag="ACHKAY_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5698964.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16280..17758)
                     /gene="scpC"
                     /locus_tag="ACHKAY_000222"
     CDS             complement(16280..17758)
                     /gene="scpC"
                     /locus_tag="ACHKAY_000222"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO5698965.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17782..18567)
                     /gene="scpB"
                     /locus_tag="ACHKAY_000223"
     CDS             complement(17782..18567)
                     /gene="scpB"
                     /locus_tag="ACHKAY_000223"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO5698966.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18578..19573)
                     /gene="meaB"
                     /locus_tag="ACHKAY_000224"
     CDS             complement(18578..19573)
                     /gene="meaB"
                     /locus_tag="ACHKAY_000224"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO5698967.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19566..21710)
                     /gene="scpA"
                     /locus_tag="ACHKAY_000225"
     CDS             complement(19566..21710)
                     /gene="scpA"
                     /locus_tag="ACHKAY_000225"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO5698968.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21914..22807)
                     /gene="argP"
                     /locus_tag="ACHKAY_000226"
     CDS             complement(21914..22807)
                     /gene="argP"
                     /locus_tag="ACHKAY_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO5698969.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22949..23134
                     /locus_tag="ACHKAY_000227"
     CDS             22949..23134
                     /locus_tag="ACHKAY_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698970.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23190..23849
                     /gene="rpiA"
                     /locus_tag="ACHKAY_000228"
     CDS             23190..23849
                     /gene="rpiA"
                     /locus_tag="ACHKAY_000228"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO5698971.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24105..25337
                     /gene="serA"
                     /locus_tag="ACHKAY_000229"
     CDS             24105..25337
                     /gene="serA"
                     /locus_tag="ACHKAY_000229"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5698972.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25566..25625
                     /gene="ibsC"
                     /locus_tag="ACHKAY_000230"
     CDS             25566..25625
                     /gene="ibsC"
                     /locus_tag="ACHKAY_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO5698973.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25958..26017
                     /locus_tag="ACHKAY_000231"
     CDS             25958..26017
                     /locus_tag="ACHKAY_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5698974.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26118..26720)
                     /gene="fau"
                     /locus_tag="ACHKAY_000232"
     CDS             complement(26118..26720)
                     /gene="fau"
                     /locus_tag="ACHKAY_000232"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO5698975.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26741..26924)
                     /gene="ssrS"
                     /locus_tag="ACHKAY_000233"
     ncRNA           complement(26741..26924)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKAY_000233"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26966..27295)
                     /gene="zapA"
                     /locus_tag="ACHKAY_000234"
     CDS             complement(26966..27295)
                     /gene="zapA"
                     /locus_tag="ACHKAY_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5698976.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27463..28041
                     /gene="ygfB"
                     /locus_tag="ACHKAY_000235"
     CDS             27463..28041
                     /gene="ygfB"
                     /locus_tag="ACHKAY_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO5698977.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28067..29392
                     /gene="pepP"
                     /locus_tag="ACHKAY_000236"
     CDS             28067..29392
                     /gene="pepP"
                     /locus_tag="ACHKAY_000236"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO5698978.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29389..30567
                     /gene="ubiH"
                     /locus_tag="ACHKAY_000237"
                     /gene_synonym="visB"
     CDS             29389..30567
                     /gene="ubiH"
                     /locus_tag="ACHKAY_000237"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO5698979.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30590..31792
                     /gene="ubiI"
                     /locus_tag="ACHKAY_000238"
                     /gene_synonym="visC"
     CDS             30590..31792
                     /gene="ubiI"
                     /locus_tag="ACHKAY_000238"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO5698980.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31866..31949)
                     /locus_tag="ACHKAY_000239"
     CDS             complement(31866..31949)
                     /locus_tag="ACHKAY_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698981.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32240..33334
                     /gene="gcvT"
                     /locus_tag="ACHKAY_000240"
     CDS             32240..33334
                     /gene="gcvT"
                     /locus_tag="ACHKAY_000240"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO5698982.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33358..33747
                     /gene="gcvH"
                     /locus_tag="ACHKAY_000241"
     CDS             33358..33747
                     /gene="gcvH"
                     /locus_tag="ACHKAY_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO5698983.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33866..36739
                     /gene="gcvP"
                     /locus_tag="ACHKAY_000242"
     CDS             33866..36739
                     /gene="gcvP"
                     /locus_tag="ACHKAY_000242"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO5698984.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37000..37743
                     /gene="ygfF"
                     /locus_tag="ACHKAY_000243"
     CDS             37000..37743
                     /gene="ygfF"
                     /locus_tag="ACHKAY_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5698985.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37800..39233)
                     /gene="bglA"
                     /locus_tag="ACHKAY_000244"
     CDS             complement(37800..39233)
                     /gene="bglA"
                     /locus_tag="ACHKAY_000244"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO5698986.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39278..39589
                     /gene="yqfB"
                     /locus_tag="ACHKAY_000245"
     CDS             39278..39589
                     /gene="yqfB"
                     /locus_tag="ACHKAY_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO5698987.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39753..40412
                     /gene="yqfA"
                     /locus_tag="ACHKAY_000246"
     CDS             39753..40412
                     /gene="yqfA"
                     /locus_tag="ACHKAY_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO5698988.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40608..41588)
                     /gene="ygfZ"
                     /locus_tag="ACHKAY_000247"
     CDS             complement(40608..41588)
                     /gene="ygfZ"
                     /locus_tag="ACHKAY_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO5698989.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41831..42097
                     /gene="sdhE"
                     /locus_tag="ACHKAY_000248"
     CDS             41831..42097
                     /gene="sdhE"
                     /locus_tag="ACHKAY_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO5698990.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42078..42485
                     /gene="ygfX"
                     /locus_tag="ACHKAY_000249"
     CDS             42078..42485
                     /gene="ygfX"
                     /locus_tag="ACHKAY_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO5698991.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42525..43046)
                     /gene="fldB"
                     /locus_tag="ACHKAY_000250"
     CDS             complement(42525..43046)
                     /gene="fldB"
                     /locus_tag="ACHKAY_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO5698992.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43158..44054
                     /gene="xerD"
                     /locus_tag="ACHKAY_000251"
     CDS             43158..44054
                     /gene="xerD"
                     /locus_tag="ACHKAY_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO5698993.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44079..44789
                     /gene="dsbC"
                     /locus_tag="ACHKAY_000252"
     CDS             44079..44789
                     /gene="dsbC"
                     /locus_tag="ACHKAY_000252"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO5698994.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44795..46528
                     /gene="recJ"
                     /locus_tag="ACHKAY_000253"
     CDS             44795..46528
                     /gene="recJ"
                     /locus_tag="ACHKAY_000253"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO5698995.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46619..47717
                     /gene="prfB"
                     /locus_tag="ACHKAY_000254"
     CDS             join(46619..46693,46695..47717)
                     /gene="prfB"
                     /locus_tag="ACHKAY_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO5698996.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47727..49244
                     /gene="lysS"
                     /locus_tag="ACHKAY_000255"
     CDS             47727..49244
                     /gene="lysS"
                     /locus_tag="ACHKAY_000255"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5698997.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49287..49835)
                     /gene="idi"
                     /locus_tag="ACHKAY_000256"
     CDS             complement(49287..49835)
                     /gene="idi"
                     /locus_tag="ACHKAY_000256"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO5698998.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            49890..49961
                     /locus_tag="ACHKAY_000257"
     CDS             49890..49961
                     /locus_tag="ACHKAY_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5698999.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49958..50083)
                     /gene="yqfG"
                     /locus_tag="ACHKAY_000258"
     CDS             complement(49958..50083)
                     /gene="yqfG"
                     /locus_tag="ACHKAY_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO5699000.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50085..51533)
                     /gene="uacT"
                     /locus_tag="ACHKAY_000259"
     CDS             complement(50085..51533)
                     /gene="uacT"
                     /locus_tag="ACHKAY_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO5699001.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIHLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51969..53888
                     /gene="ygfT"
                     /locus_tag="ACHKAY_000260"
     CDS             51969..53888
                     /gene="ygfT"
                     /locus_tag="ACHKAY_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO5699002.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53888..54376
                     /gene="ygfS"
                     /locus_tag="ACHKAY_000261"
     CDS             53888..54376
                     /gene="ygfS"
                     /locus_tag="ACHKAY_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5699003.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54412..55779)
                     /gene="ghxQ"
                     /locus_tag="ACHKAY_000262"
                     /gene_synonym="ygfQ"
     CDS             complement(54412..55779)
                     /gene="ghxQ"
                     /locus_tag="ACHKAY_000262"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO5699004.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55815..57131)
                     /gene="guaD"
                     /locus_tag="ACHKAY_000263"
     CDS             complement(55815..57131)
                     /gene="guaD"
                     /locus_tag="ACHKAY_000263"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO5699005.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57149..58549)
                     /gene="xanQ"
                     /locus_tag="ACHKAY_000264"
     CDS             complement(57149..58549)
                     /gene="xanQ"
                     /locus_tag="ACHKAY_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO5699006.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58714..61584)
                     /gene="xdhD"
                     /locus_tag="ACHKAY_000265"
     CDS             complement(58714..61584)
                     /gene="xdhD"
                     /locus_tag="ACHKAY_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO5699007.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61581..62360)
                     /gene="ygfM"
                     /locus_tag="ACHKAY_000266"
     CDS             complement(61581..62360)
                     /gene="ygfM"
                     /locus_tag="ACHKAY_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO5699008.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62411..63739)
                     /gene="ssnA"
                     /locus_tag="ACHKAY_000267"
     CDS             complement(62411..63739)
                     /gene="ssnA"
                     /locus_tag="ACHKAY_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO5699009.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(63742..66840)
                     /gene="ygfK"
                     /locus_tag="ACHKAY_000268"
     CDS             complement(63742..66840)
                     /gene="ygfK"
                     /locus_tag="ACHKAY_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO5699010.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(67162..67740)
                     /gene="mocA"
                     /locus_tag="ACHKAY_000269"
     CDS             complement(67162..67740)
                     /gene="mocA"
                     /locus_tag="ACHKAY_000269"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO5699011.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            67843..68613
                     /gene="yqeC"
                     /locus_tag="ACHKAY_000270"
     CDS             67843..68613
                     /gene="yqeC"
                     /locus_tag="ACHKAY_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO5699012.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            68661..70286
                     /gene="yqeB"
                     /locus_tag="ACHKAY_000271"
     CDS             68661..70286
                     /gene="yqeB"
                     /locus_tag="ACHKAY_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO5699013.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(70327..71259)
                     /gene="arcC"
                     /locus_tag="ACHKAY_000272"
     CDS             complement(70327..71259)
                     /gene="arcC"
                     /locus_tag="ACHKAY_000272"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5699014.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(71307..72692)
                     /gene="hyuA"
                     /locus_tag="ACHKAY_000273"
     CDS             complement(71307..72692)
                     /gene="hyuA"
                     /locus_tag="ACHKAY_000273"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO5699015.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(72745..73956)
                     /gene="ygeY"
                     /locus_tag="ACHKAY_000274"
     CDS             complement(72745..73956)
                     /gene="ygeY"
                     /locus_tag="ACHKAY_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO5699016.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(74014..75210)
                     /gene="dpaL"
                     /locus_tag="ACHKAY_000275"
     CDS             complement(74014..75210)
                     /gene="dpaL"
                     /locus_tag="ACHKAY_000275"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO5699017.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(75268..76455)
                     /gene="ygeW"
                     /locus_tag="ACHKAY_000276"
     CDS             complement(75268..76455)
                     /gene="ygeW"
                     /locus_tag="ACHKAY_000276"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO5699018.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            76934..78712
                     /gene="ygeV"
                     /locus_tag="ACHKAY_000277"
     CDS             76934..78712
                     /gene="ygeV"
                     /locus_tag="ACHKAY_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO5699019.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(78752..79231)
                     /gene="xdhC"
                     /locus_tag="ACHKAY_000278"
     CDS             complement(78752..79231)
                     /gene="xdhC"
                     /locus_tag="ACHKAY_000278"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO5699020.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(79228..80106)
                     /gene="xdhB"
                     /locus_tag="ACHKAY_000279"
     CDS             complement(79228..80106)
                     /gene="xdhB"
                     /locus_tag="ACHKAY_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO5699021.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(80117..82414)
                     /gene="xdhA"
                     /locus_tag="ACHKAY_000280"
     CDS             complement(80117..82414)
                     /gene="xdhA"
                     /locus_tag="ACHKAY_000280"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO5699022.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            82829..83584
                     /gene="actS"
                     /locus_tag="ACHKAY_000281"
     CDS             82829..83584
                     /gene="actS"
                     /locus_tag="ACHKAY_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO5699023.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            83663..83736
                     /locus_tag="ACHKAY_000282"
     tRNA            83663..83736
                     /locus_tag="ACHKAY_000282"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83695..83697,aa:Gly,seq:ccc)
     gene            84183..84440
                     /locus_tag="ACHKAY_000283"
     CDS             84183..84440
                     /locus_tag="ACHKAY_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699024.1"
                     /translation="MVSIVYNNLTIPLHEVDMTVSYKITYEHRDGDQIRMEEVLFHSE
                     HEPTKDEIHDAVRKDTVRFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(84474..84794)
                     /gene="yecR"
                     /locus_tag="ACHKAY_000284"
     CDS             complement(84474..84794)
                     /gene="yecR"
                     /locus_tag="ACHKAY_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014885115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR family lipoprotein"
                     /protein_id="HFO5699025.1"
                     /translation="MRKSLMLLLASTLIISGCATKKQLVPTGGSKSDGTVRMSYSYGM
                     FESPVVDPQQGMNAAKARCAAWGYSGAEPFGGFTSQCSQPSSSGCMQTLVTIEYQCTG
                     DIKK"
     gene            complement(85447..85998)
                     /locus_tag="ACHKAY_000285"
     CDS             complement(85447..85998)
                     /locus_tag="ACHKAY_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5699026.1"
                     /translation="MANIGYIRVSTVQQNTDRQLAGVALDKVFEDKASGKNTQRPQFE
                     AMMNYVREGDVLHVHSIDRLCRNTADLLATVEQLTERGVSVHFHKEDFKTGKNSPAGN
                     MMLTVLAAVAQMEREMMLERQREGIAAAKAAGRIAKRGNGKAIDRAGIAAALANGGSI
                     RSVAKDFSVSTQTVQRIKKEQEA"
BASE COUNT        21240 a        21503 c        22916 g        21519 t
ORIGIN      
        1 catcggtttc cctgctcaat tgctttcatt aaaccgcacg agtattttat ttttaccgcc
       61 ggacggtacc ctaaaaataa aataaggata atttatgcgg cttagtgatt attttccaga
      121 atcatcaatc tcagttatac attcagcaaa agattggcag gaagctatcg atttctcgat
      181 ggtatcattg ctggataaaa actatatcag cgagaattac attcaggcaa ttaaagattc
      241 caccattaac aatggccctt attatattct cgcaccaggc gtggcaatgc ctcatgcgcg
      301 accggaatgt ggggcgctta aaaccgggat gtctttgaca ttacttgaac aaggtgttta
      361 ttttccgggg aatgacgaac caattaaatt actcatcgga ctctctgctg ccgatgccga
      421 ttcgcacatt ggcgctattc aggcgttaag tgaattactg tgcgaagaag aaatactcga
      481 acaactctta acagcatcat cagaaaaaca attagcggac attatcagcc gcggataatt
      541 actctctcct ctacaggata atattatgga aaacaagtct gctcgtgcaa aggtccaggc
      601 ttttgggggc tttttgactg caatggtcat ccccaatatt ggtgctttta ttgcctgggg
      661 ttttattact gcgttattta ttcccaccgg ttggctgcct aacgaacatt tcgccaaaat
      721 tgtcggcccg atgattacct atttattgcc cgtgatgatt ggttctacag gtggtcatct
      781 ggtcggcggt aaacgcgggg cggtcatggg cggaataggt actattggtg tgatcgttgg
      841 cgcagagatc ccgatgttcc ttggctcaat gatcatgggg ccgctcggtg ggttggtcat
      901 aaaatatgtc gataaggcac tggaaaaacg catacctgcc ggttttgaga tggttatcaa
      961 taacttctca ttaggtatcg cggggatgct cctttgtctg ctgggttttg aagtgatcgg
     1021 cccggcggtg ttaattgcca atactttcgt caaagagtgt atcgaggcgc tggtacatgc
     1081 gggttatctg cctctgctgt cagtcatcaa tgaaccggcg aaagtgcttt tccttaataa
     1141 tgcgatcgat cagggcgtct attatccgct gggaatgcaa caggcttcgg ttaacggtaa
     1201 atccatcttc tttatggtgg cctctaaccc aggtccgggc ctggggctgc tgctggcgtt
     1261 taccttgttt ggtaaaggga tgagtaaacg ttctgcgccc ggggcgatga ttattcactt
     1321 cctcggtggg atccacgaac tgtatttccc atatgtgctg atgaagccgc tgactattat
     1381 tgccatgatt gcgggcggta tgtctggtac ctggatgttt aacttactgg acggtggtct
     1441 ggtggctggc ccaagtccgg ggtctatctt tgcttacctg gcactgacgc cgaaaggctc
     1501 gttcctggcg acaattgccg gtgttacggt aggtaccctg gtgtcctttg ctattacttc
     1561 gctgatactg aagatggaaa aaacggtgga aacggagagc gaagatgagt ttgctcagtc
     1621 agccaatgcg gttaaggcga tgaaacaaga gggtgcattc tcgttaagca gggttaagcg
     1681 tatcgccttt gtttgcgatg cggggatggg ctccagtgcg atgggcgcga ccaccttccg
     1741 taaacgcctg gaaaaagcgg ggctggcaat agaagtaaaa cattacgcga tagaaaacgt
     1801 gcctgcggat gccgatatcg tcgtcactca cgccagtctg gaagggcgcg tgaaacgtgt
     1861 gacggataaa ccgctgatat tgattaataa ctatattggc gatccaaaac tcgacacttt
     1921 atttaatcaa ttaaccgccg aacataaaca ctgattacag aggtaaaaat gaaaaccaaa
     1981 gttgctgcta tttatggcaa gcgggatgtc cgtctgcgcg aatttgaact gccagaaatt
     2041 accgataatg aactgttagt gagtgtaatt tctgacagcg tctgtttatc gacctggaaa
     2101 gcggcgttac tcggtagtga acataaacgc gtacccgacg atttagaaaa tcatccggtc
     2161 attaccgggc atgaatgtgc cggggttatt gtcgaagtgg gtaaaaatct cactggcaaa
     2221 tataaaaaag gccagcgttt tgtattgcaa ccggcgatgg ggttaccaag cggatattca
     2281 gcaggctaca gctacgaata ttttggcggt aacgccacct atatgattat tcccgaaatc
     2341 gccattaatt tgggctgcgt attaccgtat cacggctctt attttgctgc ggcgtcgctg
     2401 gcagagccta tgtgctgcat tattggtgct tatcatgcca attatcacac cacgcaatat
     2461 gtttatgagc atcgcatggg cgtcaaacct ggcggcaata ttgcactgct ggcgtgtgcg
     2521 ggtccgatgg gcattggcgc tatcgattac gccattaacg gtggcataca accgtcacgg
     2581 gtggtggtgg tcgatatcga cgacaaacgc ctggcgcagg tgcagaagct gctgccggtg
     2641 gatctggcgg ccagtaaagg cattgagctg gtgtatgtga ataccaaagg gatgagcgat
     2701 ccggtccaga cgctgcgggc gctgacagga gatgtcgggt tcgatgacat ttttgtttat
     2761 gcggcggtgc ctgctgtcgt tgagatggct gacgaattac tggcggaaga tggctgtctg
     2821 aacttctttg ccgggccgac ggataaaaac ttcaaagtgc cgtttaattt ctacaacgtc
     2881 cattacaata gcacgcacgt agtcggtaca tccggcggtt caacggacga catgaaagag
     2941 gcgattgcgt taagcgccac ggggcagttg cagccgtcct ttatggtcac gcatatcggt
     3001 gggctggatg cggtgccaga taccgtgctc aatctgccgg atatccctgg cggtaaaaaa
     3061 ctcatttata acggcgtgac catgccgctc actgccattg ccgattttgc cgaaaaaggc
     3121 aaaaccgatc cgctgtttaa agagttggcg cggctggttg aggaaacgca cggcatctgg
     3181 aatgaacagg ccgagaaata tctgctggca caatttggcg ttgatatcgg ggaggccgcg
     3241 caatgatgtc cctggcgtgg ccattatttc gcgttacgga acaggcagcg ttggctgcct
     3301 ggccgcaaac cggatgtggc gacaaaaata aaattgatgg cctggcggtc accgcgatgc
     3361 gccaggcatt aaacgacgtc gcttttcgtg gacgagtggt tatcggcgaa ggagaaattg
     3421 accatgcgcc gatgctgtgg attggcgaag aggtgggcaa aggcgatggg ccagaagtcg
     3481 atatcgcggt tgacccgatt gaaggcacgc ggatggtagc gatggggcag agcaatgcgc
     3541 tggcggtcat ggctttcgcc cctcgagata gcctgttaca tgcgcccgat atgtatatga
     3601 aaaagctggt tgttaatcga ctggctgccg gggcgattga tctgtctttg cctctggcgg
     3661 acaacttgcg taacgtggcg cgggcgttag gtaaaccgct ggacaagctg cgcatggtta
     3721 cgctggataa accgcgcctg agtgccgcga ttgaagaagc aacacagctg ggcgtgaagg
     3781 ttttcgctct gccggatggc gatgtcgccg ccagcgtgct gacctgctgg caggataatc
     3841 cttacgacgt gatgtacacc atcggcggtg cgccagaagg cgtgatttct gcctgtgccg
     3901 ttaaagcatt aggtggggat atgcaggccg agctgattga cttttgtcag gctaaagggg
     3961 attacacgga aaatcggcag attgcagagc aggagcgcaa gcgttgtaag gcaatgggcg
     4021 ttgacgttaa ccgtgtgtac tcgctcgatg aactggtgag ggggaacgat atcctcttta
     4081 gcgctacggg cgtgacgggc ggcgagttgg tgaacggtat ccaacagacg gcgaatgggg
     4141 tgcggacgca gacattactg atcggcggcg cggaccaaac gtgtaatata atagactccc
     4201 tgcattgatg gtgatctatc ggaagaccaa tggcgacgct gacagaagat gatgtgcttg
     4261 agcaactgga tgcacaggac aatttatttt catttatgaa aactgcgcat tctattttgc
     4321 tccaggggat acgccagttt ctgccgtcgc tgtttgtcga taacgatgaa gagatcgtcg
     4381 aatatgcagt gaagccgtta ctcgcccaaa gcggcccgct tgacgatatt gatgttgcgc
     4441 tgcgtttgat ttatgcgctg gggaaaatgg ataaatggct gtatgccgat atcacgcact
     4501 tttcccagta ctggcattac ctgaacgaac aggatgaaac gcccggattt gccgatgaca
     4561 tgacctggga ttttatcagc aacgtcaata gcataacctg caatgcgacg ctctatgacg
     4621 cgttaaaagc gatgaagttc gccgatttcg ccgtctggtc agaggcgcgt tttagcggaa
     4681 tggtcaaaac ggcgctgacg ctggcagtaa cgaccacctt aaaggaatta acgccgtgaa
     4741 aattgaatta acggtgaatg ggctgaacgt gcaagcgcag taccatgatg aagagataga
     4801 acgcgtgcat aaaccgttgc tgcgtatgct ggcggcctta cagacggtaa gtccgcagcg
     4861 acgtacggtg gtttttcttt gcgctccgcc ggggacgggt aaatctacct taactacctt
     4921 ctgggaatat ctcgcgcaac aagaccctga attacccgct atccagacgc tcccgatgga
     4981 tggttttcac cattacaata gctggctgga tgcgcatcaa ttgcgcccct tcaaaggcgc
     5041 accagagaca tttgacgttg cgaaactggc ggaaaatctg tgccaggtag tggaagggga
     5101 ttgtacgtgg ccgcagtatg atcgacaaaa gcatgatcct gttgaagatg cgttgcacgt
     5161 taccgcacca ctcgtcatcg tcgaaggaaa ctggttgttg ctggacgacg agaagtggtg
     5221 ccagcttgcg caattttgtg atttctcgat ctttatcaac gcgccagcta ctgcgttgcg
     5281 ggaacgactg gtagggcgca agctggcggg aggggtatcg ctggcagacg cagaagcttt
     5341 ttatgaccgt accgacgggc cgaatgttcg ccgggggctg gaagagagtc tgcctgcaaa
     5401 tttgacctta atgatgaccg ctacgggtga atatcgtctg gtggattaac gttcctgatt
     5461 agaccatctt ccagtcgcac cacacgagag aaatgacgcc gggtaaatgc ggcgtcatga
     5521 ctgattgcta cgacgctcgt gcctcgctgg ccgcacttct ccagccagct ttcaaacacg
     5581 ctcaaccaat tttcgtcaaa atcccgactc ggctcgtcca gcagcaatag tggcggagag
     5641 agtgcttcca ggcaggcgac ggcgaccatc cgtcgctgcg cactatgcag atccaacggg
     5701 tgcgagttgg cagtgtctgc taactgacaa cattgcaggg cagcatgggt acgttgagtg
     5761 atttcatccg ccgggcattt ttgcagtttc aaaccaaatg cgatctcgtc agcgacggtg
     5821 ctgtgaaaaa gttggttttc cgcttcctga aacagtacgc caacctttgc cgcgcgctgg
     5881 cggtttttca ggtttttcat cgcctgttgt tgcagcataa ccgtaccggc tgtgggcgta
     5941 agaagacctg ccattactcg taacagcgtc gattttcccg cgccattgtc gccggtcagc
     6001 gccaaccatt caccttgttt gagttgcaga gaaatgtcgc aaagacaatc cgttgccgcg
     6061 ccaggccagc gataagatat cttatttaac gttaacatag tggctggagc gctccgtttt
     6121 gcaattgcca cgcttgctgg cagtagttca ggaaaggtgt gcgatgacgt tcaaaaagaa
     6181 taatcagcga accacgttca aacgcccagt gttgtaagcg ctgcaacagc atttcgctgg
     6241 cctgcggagt caggcggcta aaggcttcat caagaattaa gagctttggc tgcatcgcga
     6301 tggcgcaggc gatgaccacg cgctgtgtct ctccaccgga gagtgttgcc gggtgacgat
     6361 ggcgcaacgg ctggcactcg gtcagagtca gggcggcatc aatccgcgcc ataatctctg
     6421 cttcagcaag acacagattt tctggcccaa aggcaacttc ttcttccacg ctaaaggtac
     6481 agccggagag ctgcaaatag ggcgattgct ggacgagctg gatggtggcg gattgttcat
     6541 taagcgggag atgcccaatg ggcgtaccga ggagcgtccc cgtgcctgtt atctctcctg
     6601 gcagaaagtc cggataccag cccgccatca gctgtgccag cgtacttttg ccactgccgt
     6661 tgtcaccaaa gatggcgacc atccccggtt tgacatagtg aaagtcataa cagaacggag
     6721 gatgcgttga gtgtgtcggg caataacgga actgttctaa cgtaaccata tccacgctcc
     6781 gaattctgtc agcatcagca agatcatggt gtaacgcgct acgcgttgta gggtgctatc
     6841 ggctggtgcc cacaacgttg ttcgtcggtt gtggatccga aatgcccgca tatccagcgc
     6901 cgcaccacgg acagccagat cgttaagcgc attgtgtgtt agcggaataa ttaacgcggg
     6961 catcgcacgt aaacgctgat accagccctc atccagcggc acgccgcggg cacgctgcgc
     7021 ttcatgaata atcgctaatt gccgttttaa ctgctcaacg accaataacg gcccggcaaa
     7081 cagataagcg acgcctggcg gcaggcgaga agcaaacaga gcgcgaataa aacattgcac
     7141 ggggacgtac tgcatccaca actgtgaggt tgaaacaatc gccagaatcc gcaaccagag
     7201 cgtaatagca tgcgaccacc gttctggcga gcgtggcgtg ccgcttagcc attctggtag
     7261 ccagccaccg tgtaccagcc acagcccggc tcccagcgag aacatcagcc agacgacata
     7321 ttttgcccgc cgctgcgtgg cctttagcgc aataaggcag aaaaaggtcg ccgcgctata
     7381 gatcggcaaa atggtctgcg cgggcaggat aagcgtggtg caggccgcca atgcccacag
     7441 tgttaatgag gtaaatggat gcatttagcg cacggcagcc attgccggaa aatggcgcgt
     7501 ggtgcgaatc ggcagctggc gcagtaaaag ccagacaatc accgctgtaa gaattttatc
     7561 gaccaggttc gcgcctatca ccgtaatcgc caccgactcc accaggtttt gccccatcga
     7621 gtgcatccag gcaacgaaca aatcggctcc gctaccggtg acaccgccga acagcgcagt
     7681 acgcagcggt acagcgacga ccgttaccgc aagcgtaata atcacgccgc tgacaacgac
     7741 ttttggcagg gtgcgaaacc aacccgctcg cgccaggcaa ccggcgacca ggccgatgac
     7801 catcgcgacg ggtgcaaatg cggcggcgat cgggttggtc agcaatcccc agagtaaatt
     7861 ggtcagtaag ccggtcagca ttccgatcac cggaccaagc agtaccgcgc taataagtgt
     7921 gccgatggag tcgagaaata taggtaattt gaccatgctg gccagttgcc cgccgatcat
     7981 attgatggcg atagatataa cgatcaacac cagtgcctga ctggaaaagt ggctacgcgc
     8041 cataagaaaa cctctttgaa agtagttatt taacggggga aatatgcaac tcgtcgtaac
     8101 cagaaagcgc gcaaggttgg cggctaaacg ccagttcctc aaggctaccg atcaccagtt
     8161 caagcgtggt ccgcacggta cagccgggca tcatatgacc gccaagcatc gtgccgtgtg
     8221 gatcggaaac gcagagatga agatgctcac cactctgttc cagcgtgccg tttagtgaga
     8281 tcacttcgaa tgtaccgttc aggagcgtgg tgccttcttg cccggcatag cgcaaggcga
     8341 cgtcagtcag gctaccggta cagcctgcaa tccaggcggc atgaagttga tgctgctgtg
     8401 caaaagcgtg tagctgggaa aaaacttcct gcccggggag gaggcgcagt gcgtaaaagc
     8461 gtgcggagga agaatatggg tagggcaatg tcatcattgg agtactccga aactcggacg
     8521 tattgcggat aatatcgacg ctgcatcagt catattctga tataagtcag ggtttgtgac
     8581 ggcacaacga aaagattttg ctacgcttat tcgtgattct gcaaagtgga ataaattcca
     8641 caggggcggg gctgaaaacg tgacgtaagt cagataattg ctttctttga ctggacaatc
     8701 attcctttta ttccacgttt cgcttatcct agctgaagcg tttcagtcga ttaaatgttc
     8761 gacaattaac caatcagtcg cagtttgcga caggtaaggt ttccccggac gatttgctgg
     8821 attactctgt ctggcaaatt tgctcaggga aaaccttgca ggagatctat gaccgtacgc
     8881 gtagcgataa atggcttcgg tcgcatcggg cgtaatgtgg ttcgtgcttt gtatgaatcc
     8941 ggacgccggg cggaaattac cgtggtggca atcaacgaac tggcggatgc tgcgggcatg
     9001 gcgcatttgt tgaaatatga caccagccat ggccgttttg catgggaagt acgacaggaa
     9061 cgcgatcaac tttttgttgg tgatgacgcc atccgcgtat tgcatgaacg ttcactgcaa
     9121 tcgctcccct ggcgtgaact tggcgttgat gtagtcctcg actgcaccgg cgtatatggc
     9181 tcccgcgagc atggcgaagc gcatattgcc gccggggcca aaaaagtgct cttttcacat
     9241 cctggcagta acgatctcga cgcgaccgtt gtttacggcg tcaatcagga tcaacttcgt
     9301 gcggaacacc gcatcgtttc taacgcttcc tgtaccacga attgcataat tcccgtcatc
     9361 aaattgttag atgatgcgta cggtattgag tccggcactg tgaccacaat tcactccgcc
     9421 atgcacgatc aacaggttat tgatgcatac catcctgacc tgcgtcgcac ccgggcagcc
     9481 agccagtcga tcattccggt cgatactaaa ctggccgccg gtatcacacg attttttccg
     9541 caatttaacg atcgctttga agcgattgcg gtacgtgtgc caaccataaa tgtgacggca
     9601 atcgatttaa gcgtgacggt gaagaaacct gtaaaagcca atgaagtcaa cctgttgctg
     9661 caaaaagcag cacaaggtgc atttcatggt atagttgact atacggaatt gccgttggtc
     9721 tctgtagatt ttaaccacga tccgcacagt gccattgtcg atggcaccca aacccgggtc
     9781 agtggcgcac acctgatcaa aacgttggtc tggtgcgata acgaatgggg ctttgctaac
     9841 cgaatgctcg acacgacgtt agctatggct actgttgctt tcaggtaaga cgcaagcagc
     9901 gtctgcaaaa cttttagaat caacgagagg attcaccatg tctgtaatta agatgaccga
     9961 tctggatctt gctgggaaac gtgtatttat ccgtgcggat ctgaacgtac cagtaaaaga
    10021 cgggaaagta accagcgacg cgcgtatccg tgcttctctg ccgaccatcg aactggccct
    10081 gaaacaaggc gcaaaagtga tggtaacttc ccacctgggt cgtcctaccg aaggcgagta
    10141 caacgaagaa ttctctctgc tgccggttgt taactacctg aaagacaaac tgtctaaccc
    10201 ggttcgcctg gttaaagatt acctcgacgg cgttgacgtt gctgaaggtg aactggttgt
    10261 tctggaaaac gttcgcttca acaaaggcga gaagaaagac gacgaaaccc tgtccaaaaa
    10321 atacgctgca ctgtgtgacg tgttcgtaat ggacgcattc ggtactgctc accgcgcgca
    10381 ggcttctact cacggtatcg gtaaattcgc tgacgttgcg tgcgcaggcc cgctgctggc
    10441 agctgaactg gacgcgctgg gtaaagcact gaaagaacca gctcgcccga tggtggctat
    10501 cgttggtggt tctaaagtat ctaccaaact gaccgttctg gactccttgt ctaaaatcgc
    10561 tgaccagctg attgttggtg gtggtatcgc taacaccttt atcgcggcac aaggccacga
    10621 tgtgggtaaa tccctgtacg aagctgacct ggttgacgaa gctaaacgtc tgctgaccac
    10681 ctgcaacatc ccggttccgt ctgatgttcg cgtagcaacc gagttctctg aaactgcacc
    10741 ggctaccctg aaatctgtta acgatgtgaa agctgacgag cagatcctgg atatcggtga
    10801 tgcttccgct caggaactgg ctgaaatcct gaagaatgcg aaaaccattc tgtggaacgg
    10861 tccggttggc gtgttcgaat tcccgaactt ccgcaaaggt actgaaatcg tggctaacgc
    10921 tatcgcagac agcgaagcgt tctccatcgc tggcggcggc gacactctgg cagcaatcga
    10981 cctgttcggc attgctgaca aaatctccta catctccact ggcggcggcg cattcctcga
    11041 attcgtggaa ggtaaagtac tgcctgcagt agcgatgctc gaagagcgcg ctaagaagta
    11101 aaaaatcaca gggcagggaa acctgccctt gtttcagcgc gcttttagag cacgcatttt
    11161 ttcatactct aaataattcg agttgcagga aggcgacaag agagtgaatc cccaggagct
    11221 tacataagta agtgactggg gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg
    11281 atgagtataa ggcccgacga tacaggacaa gagacatgtc taagattttt gatttcgtaa
    11341 aacctggcgt aatcactggt gatgacgtac agaaagtttt ccaggtagca aaagaaaaca
    11401 acttcgcact gccagcagta aactgcgtcg gtactgactc catcaacgcc gtactggaaa
    11461 ctgctgctaa agttaaagcg ccggttatcg ttcagttctc caacggtggt gcttccttta
    11521 tcgctggtaa aggcgtgaaa tctgacgttc cgcagggtgc tgctatcctg ggcgcgatct
    11581 ctggtgcgca tcacgttcac cagatggctg aacattatgg tgttccggtt atcctgcaca
    11641 ctgaccactg cgcgaagaaa ctgctgccgt ggatcgacgg tctgctggac gcgggtgaaa
    11701 aacacttcgc agctaccggt aagccgctgt tctcttctca catgatcgac ctgtctgaag
    11761 aatctctgca agagaacatc gaaatctgct ctaaatacct ggagcgtatg tccaaaatcg
    11821 gcatgactct ggaaatcgaa ctgggttgca ccggtggtga agaagacggc gtggacaaca
    11881 gccacatgga cgcttctgca ctgtacaccc agccggaaga cgttgattac gcatacaccg
    11941 agctgagcaa aatcagcccg cgtttcacca tcgctgcgtc cttcggtaac gtacacggtg
    12001 tttacaagcc gggtaacgtg gttctgactc cgaccatcct gcgtgattct caggaatatg
    12061 tttctaagaa acacaacctg ccgcacaaca gcctgaactt cgtattccac ggtggttccg
    12121 gttctactgc tcaggaaatc aaagactccg taagctacgg cgtagtgaaa atgaacatcg
    12181 ataccgatac ccaatgggca acctgggaag gcgttctgaa ctactacaaa gcgaacgaag
    12241 cttatctgca gggtcagctg ggtaacccga aaggcgaaga tcagccgaac aagaaatact
    12301 acgatccgcg cgtatggctg cgtgccggtc agacttcgat gatcgctcgt ctggaaaaag
    12361 cattccagga actgaacgcg atcgacgttc tgtaagtcat tcctttctgc ttatctcaag
    12421 gcccgctctg cgggtctttt tttcgccaaa agcaaaattg cctgatgcgc tacgcttatc
    12481 aggcctacac gtatcctgca atatattgaa tttgcaggtt tttgtaggcc ggataaggcg
    12541 ttcacgccgc atccggcatg acaaagcgca cattgatatc tgtctgagcc ccgctctgcg
    12601 gggatttttt cgccaaaagc aaggtgattc agatgagaaa tctgtgatct atttggcaaa
    12661 attatgcttt attgtttacc cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc
    12721 tccattttga gtcagttgaa aaggaatatt gaatggaaga tttgaatgtt gtcgatagca
    12781 taaacggcgc gggaagctgg ctggtagcta accaggcgct gctgctaagt tatgcagtaa
    12841 acatcgtggc ggcactcgcg atcatcatcg ttggtttgat tatcgcgcgg atgatttcca
    12901 acgcggtgaa tcgcctgatg atctcccgta aaatcgatgc cactgttgct gattttcttt
    12961 ctgcattagt ccgttacggt attatcgcct ttacgctaat cgcagccctg ggacgcgtgg
    13021 gtgtacaaac agcgtcagtc attgctgtac tcggtgccgc aggcttagct gttggtctgg
    13081 ctttgcaggg gtcactttct aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt
    13141 tccgtgccgg agaatatgtt gacctcggcg gcgtagccgg tactgtgctg agtgtgcaga
    13201 ttttctccac caccatgcgt actgcagacg gtaaaattat cgttattccg aacggtaaaa
    13261 ttattgccgg aaatattatt aacttctccc gcgagccagt tcgccgtaac gaatttatta
    13321 ttggcgtggc gtatgattcc gatatcgatc aggttaagca gatcctgacc aatattatcc
    13381 agtctgaaga tcgcattttg aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg
    13441 catcgtcgat taatttcgtg gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt
    13501 actgggatgt gctggagcgt attaaacgtg aatttgatgc cgccggtatc agcttcccgt
    13561 acccgcaaat ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg taatcaacgc
    13621 tgaggccaga taatactctc tggcctctct cttattagtt attctgattg ccaattaata
    13681 ttatcaattt ccgctaataa caatcccgcg atatagtctc tgcatcagat acttaattcg
    13741 gaatatccaa cgtgttttct tattactttc aaggtcttgc tcttggggca gctatgattc
    13801 tgccgctcgg cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca
    13861 ttatgattgc cttactttgc gctatcagcg atttggtcct gatttgcgcg gggatttttg
    13921 gtggtagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc tggggcggcg
    13981 tagtcttctt gctgtggtat ggttttggcg cttttaaaac agcgatgagt agtaatattg
    14041 agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc accatgttgg
    14101 cagtgacctg gctgaatccg catgtttacc tggatacttt tgttgtactg ggcagccttg
    14161 gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt
    14221 tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg
    14281 caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt atagccttgc
    14341 agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg
    14401 gaaaaataaa acagaggcgc taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa
    14461 ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctggcggcat taatgggtat
    14521 tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg
    14581 tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc
    14641 cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc
    14701 cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt tacgcaccca
    14761 gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg ctgtgagaac
    14821 ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg gcgcgctgaa
    14881 ggcgggtctt aacgaaattc gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa
    14941 agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa
    15001 cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt ccaactatca
    15061 gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg cccaggaaac
    15121 ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc agttagaacc
    15181 tgtggatcaa caacccgcta aaacacctgc agcacaataa tacttgtagc ccgacgtatt
    15241 cgtcgggcta atgcctttac ccgatattgc ctcctttttc tgtaattttc tgtaaaacca
    15301 tgacgtaatc acctatcttt gctcgcggtt tcatcaagga taggctgact gaaacggtta
    15361 agcggacaag gatgcgggga ggggggatgg acatttttat ttcgaaaaag atgcgcaatt
    15421 tcattttatt agcgcaaacc aataatattg cccgggcggc agaaaaaatc catatgacgg
    15481 cttcgccatt tggtaaaagc attgccgcgc tggaagaaca aattggctat acgctattta
    15541 cccgcaaaga taataatatc agcctcaata aagccggtca ggaactgtat caaaaactgt
    15601 tcccggttta tcaacgactt tctgcgattg ataatgaaat ccataactca gggcgtcgtt
    15661 cacgagaaat tgtgattggc atagataata cttatccgac aattattttc gatcagttga
    15721 ttagtctcgg agataagtat gaaggcgtga ccgcccagcc agttgagttc agcgaaaacg
    15781 gtgttatcga taatcttttt gaccgtcagt tagattttat tatctcccca cagcatgtat
    15841 ctgcacgagt tcaggaactg gaaaacctga ctatcagtga attaccaccg ctgcgattgg
    15901 gctttctcgt ttctcgccgt tatgaagagc gtcaagagca agagctgctt caggagctgc
    15961 cctggctaca aatgcgcttt caaaaccggg ctaacttcga agcaatgatt gatgcgaata
    16021 tgcgaccttg tgggatcaat cccaccatta tttatcgtcc ctacagtttt atggccaaaa
    16081 tcagcgccgt ggagcgcgga cattttctga cggtgatccc gcattttgcc tggcgactgg
    16141 tgaatccggc aacgctgaaa tattttgatg cgccgcacag gccgatgtat atgcaggaat
    16201 atctttattc aatcagaaat catcgttata ccgccacgat gcttcagcat attgctgaag
    16261 atcgtgacgg gacaaaccat taacccagca tcgagccggt tgcaattaaa ttacggtgta
    16321 agtcgaagac gtggctaaga tcgtggtgaa tatgtccgcc gggcgcattt tccagatagc
    16381 gatgcagata atcccgatac ataggatgtg cacaattatc aatgatagtg cgggcgcgtt
    16441 gaagcgggga aagaccgcgc agatcggcga tcccttgttc ggtgatgatc actttgacgc
    16501 tgtgttcgct gtgatcaaca tggctgcaca ttggcacgac ggttgagatc ttgccttctt
    16561 tagcaatcga cggagccata aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc
    16621 cgccgatgcc gttcatcaga tcgaccccgg ctacgtgcgt tgagttggca tgcccgtaaa
    16681 tatcaaactc caggccgacg ttcagagcga tgacgcccag acgacggatg atttccgggt
    16741 tattggaaat ctcctgcgga cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt
    16801 aaatcttgcg cagggaatcg gccgagattg tcaggctgga ggcgctggcc ccgctgattt
    16861 tgccggtttc cagtaaatgc accaccgatt cctgtagcac ttccgaatac atcatgaacg
    16921 gaggaatttc cgggttttcc cccagacgcg ccattaccgc attattgata ttgcccacgc
    16981 cactttgcag cggcagaaat tccggcggaa tacgcccatg cgccatttcc tgcaataaga
    17041 acgtgaccac gttatcggca atctgctggc acatgggatt ttgcttatcc agcatattac
    17101 cggcgtcggg caagttggtt tccacgacgg cgacaatctt tttcggatcg atttgcacat
    17161 agcgggtacc gacgcgatcc attgcatgga agatcgacac gctattgcgc cgcggtggcg
    17221 cgccaggaat cacaatatct gccagttctg caacgcgcgg atcgtgatag tgattaagtt
    17281 caatgatcac tttcttcgct cgcagcagcc aggtcggcgc attaccgatc ccgctggtta
    17341 accagactcg accatccggt gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc
    17401 cgaagaaacc gtaattgacc atttgcgcca cttcgctcaa atgcaggtca acgaaactca
    17461 ccgcgccctg attgatcttt ttacgtaaac cggacgatgt ttgatatggc gcacgccagg
    17521 aaacagcatc ggcgtcagaa agtacatcgt cagcggcggc gctgattgac gcccccgtca
    17581 gcaggcgaat ttgatacggc tttttggcct catgctgttc gttagctctg cgggcaatcg
    17641 cggtgggtag ggctttcggc gaacccgccg gggtaaagcc gctaaatgcc accatgtcgt
    17701 tatgctggat aatttctgcc gcttcatcgg cggtcatcct tgtccactga gtttccattt
    17761 ttactccttc gttcgcaggg attaatgacc aacgaaatta ggtttacgtt tttcgaggaa
    17821 agcgttcatt ccttcctggt aatcttcgct gtcataaacc gcgcgtcgca tcccctgaat
    17881 acgttcaaat tcatcggagt tcatggtgtg cgcttcgccc agtacacgca gctcttcttt
    17941 gataacagca atggctaacg gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa
    18001 atcttccagt tcttccactt ccacaacatg gttgaggatg ccgacagcca acgcgcgctg
    18061 ggcggtgatt ggcgaagcgg taaaaatcag ttctttgaca atgtggaagc ccgcgtcgcg
    18121 ggtcaggttg tgaatgccga ccaggttata cgggacgccg aggtttacag gcgtcattga
    18181 gaaggttgag gtactggcgg cgataatcag atcggaactc atgatcattt caaatgcgcc
    18241 accccaaaca ctaccttcca ccatcgaaat gatcggtttc gggaattttt ggatcatgcg
    18301 ggtgatttga cgcaatggat catcatagga gagcggatcg cgaccgccag acggcagttc
    18361 gtgaatatcg tggcctgcgg agaagacttt ggatccactc ggcgcgcgca aaatgataca
    18421 gcgaatttcc ggccggttga gatcgcttaa tgcctgcata agatcatcaa taaagacttt
    18481 acttaaggca ttaagtttgc ggccatagtt aaactcaatg accgccactt tgttgatagt
    18541 gacaacgtta acatactgat aagacataaa aattccttta atcaaaatat tgcgtctgga
    18601 taaattcact gagctgccgc aggccggtgc gcggtgagag cgtattgttt ttgaccgcta
    18661 aaagcgtctg gcggtaatag cgatcgaaat cttcattcgc gaacaggtga ttcagtactt
    18721 cttcttcggt ctgcttacgc agccattcca ccgattgttg ttgccgcact tgttgtaaac
    18781 gaccactggc agttagcgcg gttttgaagt cgatgatggc gtgccagatc tcatcgattc
    18841 cacgtttttc cagtgcgcta caagtcagaa cccgtggctg ccattcgtcg tatttacgtc
    18901 gcagaatatg cagggcactc tcgtacatat gccgggcaat ggcgacattg gtatggttat
    18961 cgccatcgtc tttgttgata acgatcagat cagccacttc catcagccct tttttaatgc
    19021 cctgcagatc atcgccgcca ccggcaattt gcaacgagat aaaacagtcc accatgcggg
    19081 cgacttctgt ttccgactgc ccgacgccaa ccgtttcgac aatcactacg tcataacccg
    19141 ctgcttcgca taacagcatt aattcccgcg ctcgctgact ggcaccgccc agatgaccgg
    19201 aggatggtac cgggcgaata aacgccgctt cggcacgcgc caggtcattc atgcgggttt
    19261 tatccccgag aatgctaccg ccagtgaccg ggctgctggg atcgaccgca ataaccgcga
    19321 cctttaatcc ctctcgaatc aacaacatgc caaaggcctc aagaaaggta cttttccccg
    19381 cgccgggggt gccggtaacg cccagtcgca gggtgttacc gcagtacggc ataatggcat
    19441 caagcagctg cgtacttagt gcctgatgac gcgggtgacg gctttccacc agcgtcatgg
    19501 cctgggcgag tgtggcacgc tcaccctgac gtaagcggcg aatactttct gccagcgtgg
    19561 cttcattaat catgatgctg gcttatcaga ttcagtacgt cgcgcacact gtcgagcata
    19621 ggtgtacctg gaccataaat cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc
    19681 ggcggaatga cgccacccgc gaccacgcag atatcttcgc gtccccattt tttcagcgct
    19741 tcgaccagtt ccgggatcag cgttttatga ccggcagcca gtgaggatgc gcccactacg
    19801 tgaacgtcgt tttctacggc caggcgggcg atctcttcag gtgtagagaa catcgggctt
    19861 aaatctacgt cgaaaccgag atcggaatag gcgctggcga tcactttcgc gccgcgatcg
    19921 tgtccatcct ggcccatctt agcgatcaga atgcgcgggc gacgaccatt gtcggcaagg
    19981 aactgctccg tttgcgcaac aatggcatcg aactcggagg ccgatttctc agactgatga
    20041 tagctttgcg caatcacgcc ggtaacacac tggcttggca ccagataacg gtcgaaagcg
    20101 acttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg
    20161 gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt
    20221 acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc
    20281 accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg
    20341 ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc
    20401 tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg
    20461 tcgatctgtt ggataatagc tctggcttgt ttgacgattt gatcggtcag cgactcaatg
    20521 taataggatc cggccagtgg atcgacggtg cggcagagtt ctgattcttc ctggatgatg
    20581 atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca
    20641 aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagtgc ttcaatggtg
    20701 gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag
    20761 tgggtacgca gcgccagtga tttcggatcc tgtgcgccaa atccactgac cgcttcgctc
    20821 cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat gccgataccg
    20881 aagaagaacg acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct
    20941 ttgatgtact caatcccatc agcgagcgta aatgctacct gctgcacgca gttggcaccc
    21001 gcttccccca tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa
    21061 caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggtgggta aatataggtg
    21121 ttgcggcaga ggtactcttt gaggatatcg ttctgaatgg tgccggtcag tttatcaggt
    21181 gtaacacctt gctcttctgc ggcgacgata taaaacgcca gtactggtag cactgcgcca
    21241 ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata
    21301 tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cgcccgccac gcgcgggtta
    21361 tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg
    21421 gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac
    21481 tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc
    21541 ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc
    21601 ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga
    21661 cggctcaatt ccttgttggc aagctgttgc cactcctgca cgttagacat tatttgctcc
    21721 gtaaaacgca atcaaaatga gggtatttgc gcctacggtg agctttttac gtgggtttgt
    21781 cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg
    21841 tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt gctatcaggc
    21901 attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt aacgcatcag
    21961 tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccgtcgtt
    22021 gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc tcgatttgca
    22081 ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag
    22141 aattaacgat atggcagggc acgctgcctg gaggcagatc gaagttttgc tgcaaaaagg
    22201 cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcagt aatgccgaac
    22261 gcgttacgcc gttagggaaa tatttttcgg caaagggttt tgagctgacg aacagatagt
    22321 cgagcgcacc aagtttatcg acaagacaac tcggcagcgc ctgatgttga atactcaccg
    22381 cgccgaccac ttcgccgcgg cgcagacgtt cctgagtgcg ggtttcatct tctacctgca
    22441 agttgaggcg gataggcgaa tcagccaaca caggagccag tgcaggaagc aaccacgtcg
    22501 ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat
    22561 cgcccagcca ctcttcttcc agcaactcca cctggcgcag cagtgccagc agtttttgcc
    22621 cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt
    22681 ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc
    22741 gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc
    22801 gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cacaattttg
    22861 tgtcaggttg catataccga agccgtcggc gggtaacatt tgtattcgtg gttaaacgcg
    22921 cagcttaaaa gatcagggat ttgcgctaat gcatttcgct cagcgagttc gcgccctggt
    22981 tgtgttgaat ggcgtggcgt tattgcctct gttcgcaccg tggagcggca tacccagacc
    23041 gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat attttatgga
    23101 tgagttaacc acgcggcttg ccaacggggt ctgaatcgct ttttttgtat ataatgcgtg
    23161 tgaaatttca taccacaggc gaaacgatca tgacgcagga tgaattgaaa aaagcagtag
    23221 gatgggcggc acttcagtat gttcagcccg gcaccattgt tggtgtaggt acaggttcca
    23281 ccgccgcaca ctttattgac gcgctcggta caatgaaagg ccagattgaa ggggccgttt
    23341 ccagttcaga tgcttccact gaaaaactga aaagcctcgg cattcacgtt tttgatctca
    23401 acgaagtcga cagccttggc atctacgttg atggcgcaga tgaaatcaac ggccacatgc
    23461 aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa aatcattgct tcggttgcag
    23521 aaaaatttat ctgtattgca gacgcttcca agcaggttga tattctgggt aaattcccgc
    23581 tgccagtaga agttatcccg atggcacgta gtgcagtggc gcgtcagctg gtgaaactgg
    23641 gcggtcgtcc ggaataccgt cagggcgtgg tgaccgataa tggcaacgtg atcctcgacg
    23701 tccacggcat ggaaatcctt gacccgatag cgatggaaaa cgccataaat gcgattcctg
    23761 gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg attggcacac
    23821 ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg aacctccccc gttaaaaaaa
    23881 ttctcttcat taaatttggt gacatgtgtc acgcttttac caggcaattg tcgattgctc
    23941 taaataaatc ctctaaacca gcatattcat ccaagaatta cctttgcgtg atatttcctc
    24001 aacatcgcga cgcaaacgtt catattgccg caatattatt ttttgatatg ttgaaaggcg
    24061 gatgcaaatc cgcacacaac atttcaaaag acaggattgg gtaaatggca aaggtatcgc
    24121 tggagaaaga caagattaag tttctgctgg tagaaggcgt gcaccaaaag gcgctggaaa
    24181 gccttcgtgc agctggttac accaacatcg aatttcacaa aggcgcgctg gatgatgaac
    24241 aattaaaaga atccatccgc gatgcccact tcatcggcct gcgatcccgt acccatctga
    24301 ctgaagacgt gatcaacgcc gcagaaaaac tggtcgctat tggctgtttc tgtatcggaa
    24361 caaaccaggt tgatctggat gcggcggcaa agcgcgggat cccggtattt aacgcaccgt
    24421 tctcaaatac gcgctctgtt gcggagctgg tgattggcga actgctgctg ctattgcgcg
    24481 gcgtgccgga agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg gcggcgggtt
    24541 cttttgaagc gcgcggcaaa aagctgggta tcatcggcta cggtcatatt ggtacgcaat
    24601 tgggcattct ggctgaatcg ctgggaatgt atgtttactt ttatgatatt gaaaacaaac
    24661 tgccgctggg caacgccact caggtacagc atctttctga cctgctgaat atgagcgatg
    24721 tggtgagtct gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc gcgaaagaga
    24781 tttcgctaat gaagcccggc tcgctgctga ttaatgcctc gcgcggtact gtggtggata
    24841 ttccggcgct gtgtgatgcg ctggcgagca aacatctggc gggggcggca atcgacgtat
    24901 tcccgacgga accggcgacc aatagcgatc catttacctc tccactgtgt gaattcgaca
    24961 acgtccttct gacgccacac attggcggtt cgactcagga agcgcaggag aatatcggcc
    25021 tggaagttgc gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc tctgcggtga
    25081 acttcccgga agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac atccacgaaa
    25141 accgtccggg cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc gtcaacatcg
    25201 ccgcgcaata tctacaaact tccgcccaga tgggttatgt agttattgat attgaagctg
    25261 acgaagacgt tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt accattcgcg
    25321 cccgtctgct gtactaattc cccttctctg aaaattaacg ggcaggtcac tgacttgccc
    25381 gtttttttat cccttctcca caccgttgcc gcgttgcgca ttctactttg cgagtctgct
    25441 ccagaaaata aatcaaagtc agatcaatgc gttgtgttta tttaatgggg atttacgatg
    25501 gcgggacagc atggggctgt aacggtaaag ccctcaccga agcgagggct taaaggagaa
    25561 gggttatgat gcgacttgtc atcatactga ttgtactgtt actgataagt ttcggagctt
    25621 attaacagtc agtctcaggg gaggagtcat cctcccttac ccttactcac taaattaggt
    25681 caaagaatca acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa ccgaactttt
    25741 tgatagccac ttcatcgccc acgtttactg ccgtaataga tcctttttcc caaaaattat
    25801 ctctgttttg catggggttt ttcgagcacg ctcgaagaaa agatttaaag gtagatcaat
    25861 gcgttgtggt tatttaaaga gagcttacga tagctgggca gcatggggct gtaacggtaa
    25921 agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg ccatcatact
    25981 gattgtactg ttactcataa gtttcccagc ttattaacag tcagtcacag gggaggagca
    26041 atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc aatcagggcg
    26101 atgcaggtgt atcgccctta ccactcccag actttcgacg gtgtaaccac cgcaggaaga
    26161 gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg cgcataaccc
    26221 accggttgcg ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa accaccgccc
    26281 attcccaggc gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag cacgtctaat
    26341 cgggaaaggg gtagcacgtc acgcacatcc agttttggct catggatctt caacctgttc
    26401 atcaccagtt cgctttgcgg atggtaattc aggaacagca aattaccggc actaaaggga
    26461 tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat tagcggctgg
    26521 gtgtcgagtt caccatcaaa agagaggaat acagcgaccg tatgcgccat caccaccggg
    26581 ggataagtca tcatccgggt agcggcttgt tgacccattt cctgctgttg ttccggcgtt
    26641 aacgcacgac gacgttgccg aatcattttg cggatttctt gtcgggataa tgtcagtggg
    26701 agttctggta gttgcgtcat ggctggtgcc agataagaag ggaatctccg agatgccgcc
    26761 gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct
    26821 tctcggacgg accgagcatg ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat
    26881 atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc ttcacggtta
    26941 ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt tcggtgatgc
    27001 gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata cgctgttcca
    27061 tacttgccgc gtagtcacga gtcttcgctt tttcttgcgc taactcatag ctgatattca
    27121 atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct ttcagatctt
    27181 gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg tcaggcgggc
    27241 agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca gacatgccac
    27301 cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg aaggggcgac
    27361 actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg gcccaaagtg
    27421 gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat acagaacgaa
    27481 atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg tctgacccct
    27541 gctgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga cagctcatgg
    27601 ctaccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga gctggcacag
    27661 gcactgcgta aaatgcactc tgccaccagc gatgccctgc aggatgacgg cttccttttt
    27721 cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga tgcattggca
    27781 ggttgggtca atcacttcct gcttggtctt ggcgttacgc aaccgaagct ggataaagtg
    27841 accggcgaaa ccggtgaagc tatcgacgat ctgcgtaaca ttgcgcaact gggttacgac
    27901 gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga atacgttcgt
    27961 gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc gccagaagta
    28021 caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga gtgagatatc
    28081 ccggcaagag tttcagcgtc gccgtcaggc cctggtggag caaatgcaac ccggcagcgc
    28141 cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat acccctatcg
    28201 tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct
    28261 gattaaaagc gatgacactc ataaccacag cgttctgttt aaccgcgttc gcgacctgac
    28321 ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt
    28381 tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt tatcaactac ttaacggcct
    28441 ggatgtggtt taccatgccc agggcgaata tgcatatgct gatgaaatcg tgaacagtgc
    28501 gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa cgatgatcga
    28561 ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact
    28621 ccgccgcgcg ggagaaatca ccgccatggc acatacccgg gcgatggaaa aatgccgtcc
    28681 gggaatgttc gagtaccatc tggaaggcga aattcaccac gaatttaacc gccacggtgc
    28741 gcgctatccg tcctataaca ccattgtcgg cagcggtgaa aacggctgca ttctgcacta
    28801 caccgaaaac gagtgtgaaa tgcgcgacgg cgacctggtg ttgattgacg cgggttgcga
    28861 atacaaaggt tacgctggcg atattacccg caccttcccg gtcaacggca aattcactca
    28921 ggcccagcgt gaaatctacg acattgtgct ggagtctctc gaaaccagct tgcgcctgta
    28981 tcgtccggga acttccattc tggaagtcac tggtgaagtg gtgcgcatca tggttagcgg
    29041 cctggtaaaa ctcggcattc tgaaaggtga tgttgatgaa ctgatcgctc agaacgccca
    29101 tcgtcctttc tttatgcatg gccttagcca ctggttaggg ctagatgtcc atgacgttgg
    29161 cgtttatggt caggatcgct cgcgcattct ggaaccgggg atggtactga ccgtagagcc
    29221 tggtctgtat atcgcgccgg atgcggatgt gccagaacaa tatcgcggta tcggcattcg
    29281 tattgaagac gacattgtga ttaccgaaac cggcaacgaa aacctcaccg ccagcgtggt
    29341 gaaaaagccg gaagaaatcg aagcgttgat ggcagctgcg agaaagcaat gagcgtaatc
    29401 atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac
    29461 ggggcgctgc cggtacattt gattgaagcg accgcgccag agtcacatgc tcatccgggc
    29521 tttgatggac gagctattgc gctggctgcg gggacctgtc agcaactggc gcgcatcggc
    29581 gtctggcaat ctctggcgga ttacgcaacc gccatcacta ccgtgcatgt cagcgatcgt
    29641 ggtcacgctg gatttgtcac cctcgccgca gaagattacc aactggcggc gctgggacag
    29701 gttgtcgaat tgcacaatgt cgggcaacgg ctgtttgcat tgctgcgtaa agcacctggc
    29761 gtaacgctgc attgccctga tcgcgtggct aacgttgccc gtactcagag tcacgttgaa
    29821 gtgacgctgg agagtggcga gacgctgacg ggccgcgtgc tggtagcagc tgatggcacc
    29881 cattcagcgt tagccaccgc gtgcggcgtt gactggcagc aggagcctta cgaacaactg
    29941 gccgtgattg ccaacgttgc gacttccgtt gcgcacgaag gccgcgcttt tgaacgcttc
    30001 acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc gctggtctgg
    30061 tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga gcgacgagaa gttttgccgt
    30121 gaactccagt cggcctttgg ctggcgactt ggaaaaatta cccacgccgg taaacgcagt
    30181 gcttatccgc tggcgttaac ccgcgccgcc agagctatta cccatcgtac cgtgctggtg
    30241 ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag ggtttaacct cggtatgcga
    30301 gatgtcatga gccttgcgga aaccctgact caggcgcatg agcgcggaga agacatcggt
    30361 gattacggca tattgtgccg ctatcagcag cgtcgacaga gtgatcgcga agcaaccatc
    30421 ggcgtcacgg acagcctggt acatcttttt gccaaccgtt gggcaccgct ggttgtcggg
    30481 cgcaacatcg gactgatgac gatggaatta ttcaccccgg cacgcgatgt gctggcgcag
    30541 cgcaccctcg gttgggtggc gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt
    30601 tgatgtagcc attgttggtg gcggcatggt ggggctggcg gttgcctgcg gcttacaggg
    30661 gagcggctta cgcgttgccg tactggagca gcgcgtaccg gaacctctgg cggcggatgc
    30721 accaccacaa ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct
    30781 tggcgtctgg caggacattc tctctcgtag ggccagttgt tatcacggta tggaagtgtg
    30841 ggacaaagac agttttggtc acatttcgtt tgacgatcaa agcatgggct atagccatct
    30901 tgggcatatc gttgaaaact cagtgattca ctacgcgctg tggaacaaag cgcagcagtc
    30961 gtcagatatc actctgttgg cccccgcaga attacagcag gtcgcctggg gagaaaatga
    31021 aactttcctg acgctgaaag atggcagcat gttaacggcg cgtctggtga ttggcgcgga
    31081 cggtgctaat tcctggttgc gcaacaaagc agatattccg ctgactttct gggattatca
    31141 gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg catgatgcgg tggcgcggca
    31201 ggttttccat ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc
    31261 aattgtctgg tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagatga
    31321 atttaatcgc gcgttaaata tcgcttttga taatcgcctg ggcttatgca aggttgagag
    31381 cgcgcgtcag gtgttcccac tgacggggcg ttatgcgcgc cagtttgccg cgcaccgtct
    31441 ggtgctggtg ggcgacgccg cgcataccat tcacccgctg gcggggcagg gagtaaatct
    31501 cggctttatg gatgctgcag agctgattgc tgaactgaaa cggttgcatc gtcaggggaa
    31561 agacatcggg cagtacattt acctgcgccg gtatgagcgt agccgcaagc acagtgcggc
    31621 gctgatgctg gctggtatgc agggattccg cgatctgttt tccggtacca atccggcgaa
    31681 aaaactgctg cgtgatattg gtttgaaact ggccgacacg cttcctggcg ttaaaccaca
    31741 acttatccgc caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct
    31801 cttctgctgt ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat
    31861 tttcctcatt tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgtca
    31921 cgaatttttg atttagtgtt ttttgacatt ttttcagctc ttaatattgc cttattcaaa
    31981 ttaactttct catcacatca tctttatata aaaacttgtg tattttttgg ttttttattc
    32041 tgttgcgatt ttggcgtttt ttaaccataa gctaatgtga tgatcaattt taccttatgg
    32101 ttaacagtct gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg
    32161 agtggctccg atgctgggtt tcgtggtgat aatttcacca tgaaaaagtt gtcagccccg
    32221 cttattcaat gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg
    32281 cggcgctcgc atggtggatt tccacggctg gatgatgccg ctgcattacg gttcgcaaat
    32341 cgacgaacat catgcggtac gtaccgatgc cggaatgttt gatgtgtcac atatgaccat
    32401 cgtcgatctt cgcggcagcc gcacccggga gtttctgcgt tatctgctgg cgaacgatgt
    32461 ggcgaagctc accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg
    32521 tgtgatagat gacctcatcg tctactactt tactgaagat ttcttccgcc tcgttgttaa
    32581 ctccgccacc cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat
    32641 cgaaattacc gttcgtgatg acctttccat gatcgccgta caagggccga atgcgcaggc
    32701 aaaagctgcc acactgttta atgatgctca gcgtcaggcg gtggaaggga tgaaaccgtt
    32761 ctttggcgtg caggcgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg
    32821 ctatgaaatt gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc
    32881 gggcgttaag ccatgcggtc tgggcgcgcg tgacacgctg cgtctggaag cgggcatgaa
    32941 tctttatggt caggagatgg acgaaactat ttctccttta gccgccaaca tgggctggac
    33001 tatcgcctgg gaaccggcag atcgtgactt catcggtcgt gaagccctgg aagtgcagcg
    33061 tgagcatggc acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa
    33121 tgaactgccg gtacgtttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag
    33181 cgggactttc tccccgacgc tgggttacag cattgcgctg gcacgcgtgc cggaaggtat
    33241 tggcgaaacg gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacgaaacc
    33301 tgtttttgtg cgtaacggta aagccgtcgc gtgatttact tttttggaga ttgattgatg
    33361 agcaacgtac cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc
    33421 gacggcactt acaccgttgg tataaccgaa catgcccagg agctgttagg cgatatggtg
    33481 tttgttgacc tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc
    33541 gaatcagtaa aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta
    33601 aacgacgcac tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggtggctgg
    33661 atctttaaaa tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca
    33721 tacgaagcat tgttagaaga cgagtaacgg ctttattcct cttctgcggg agaggatcag
    33781 ggtgaggaaa atttatgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca
    33841 ctgcacgttt caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg
    33901 cgcttttatt gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc
    33961 cgttggtgca caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcagct
    34021 tgcgaccccg ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg cagaactcaa
    34081 ggctattgcc agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt
    34141 gcagctaccg ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta
    34201 cactccgtat caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca
    34261 ggtaacgctg gatttgactg gactggatat ggcctctgct tctcttctgg acgaggccac
    34321 cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg
    34381 cttcttcgtg gcttccgatg tgcatccgca aacgctggat gtggtccgta ctcgtgccga
    34441 aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc atcaggacgt
    34501 cttcggcgtg ctgttacagc aggttggcac aaccggtgaa atccacgact acaccgcgct
    34561 tattagcgaa ctgaaatcac gcaaaattgt ggtcagtgtc gccgccgata ttatggcgct
    34621 ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt gtttttggtt cggcgcaacg
    34681 cttcggcgtg ccgatgggct acggtggccc acacgcggcc ttctttgcgg cgaaagatga
    34741 atacaaacgc tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac
    34801 cgcgctgcgc atggcgatgc agactcgcga gcaacatatt cgccgtgaga aagcgaactc
    34861 caacatttgt acttcccagg tgctgctggc aaacatcgcc agcctgtatg ccgtttatca
    34921 cggcccgatt ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atatcctggc
    34981 ggcgggcctg caacaaaaag gtctgaaact gcgccatgcg cactatttcg acaccttgtg
    35041 tgtggaagtg gccgacaaag cgggcgtact gacgcgtgcc gaagcggctg aaatcaacct
    35101 gcgtagcgat attctgaacg cggttgggat cacccttgat gaaacaacca cgcgtgaaaa
    35161 cgtaatgcag cttttcagcg tactactggg cgataaccac ggcctggaca tcgacacgct
    35221 ggacaaagac gtggctcacg acagccgctc tatccagcct gcgatgctgc gcgacgacga
    35281 aatcctcacc catccggtgt ttaatcgcta ccacagcgaa accgaaatga tgcgctatat
    35341 gcactcgctg gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg
    35401 caccatgaaa ctgaacgccg ccgccgagat gatcccaatc acttggccgg aatttgccga
    35461 actgcacccg ttttgtccgc cggaacaggc tgaaggttat cagcagatga ttgcgcagct
    35521 ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactccgg
    35581 cgcacagggc gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga
    35641 agggcatcgc gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc
    35701 acatatggca ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct
    35761 gactgatctg cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac
    35821 ttatccttct acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca
    35881 tcagttcggc ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac
    35941 ctcgccgggc tttattggcg cggacgtttc gcacctcaac ctgcataaaa ctttctgcat
    36001 tccgcacggc ggtggtggtc cgggtatggg accgatcggt gtgaaagcgc atctggcacc
    36061 gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcagt
    36121 ttctgcggca ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat
    36181 gatgggcgca gaagggctga aaaaagcaag ccaggtggca atcctcaacg ccaactatat
    36241 tgccagccgc ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc
    36301 gcacgaatgt attctcgata ttcgcccgct gaaagaagaa accggcatca gcgagctgga
    36361 tattgccaag cgcctgatcg actacggttt ccacgcgccg accatgtcgt tcccggtggc
    36421 gggtacgctg atggttgaac cgactgaatc tgaaagcaaa gtggaactgg atcgctttat
    36481 cgacgcgatg ctggctatcc gcgcagaaat cgaccaggtt aaagccggtg tctggccgct
    36541 ggaagataac ccgctggtga acgcgccgca cattcagaac gaactggtcg ccgagtgggc
    36601 gcatccgtac agccgtgagg ttgcagtatt cccggcaggt gtggcagaca aatactggcc
    36661 gacggtgaaa cgtctggatg atgtttacgg cgaccgtaac ctgttctgct cctgcgtacc
    36721 gattagcgaa taccagtaat tcactgattc gactattttc taaaggcgct tcggcgcctt
    36781 tttagtcaga tgacaaagta caaaagtgct cagacagtcc cctcgcccct ttggggagag
    36841 ggtgagggga acaggccggc actggcgcga atatttaccc tcaccccggc cctctccctg
    36901 aaagggcgag ggggaaaagc gtgccaacat tgaagattga gccagtttgt tagcaatctc
    36961 aaagatacgt caacgaatga atttttctcg gaaaaacaaa tggctatagc acttgtgacc
    37021 ggtggcagtc gcggcatcgg gcgggcaact gcattactgt tggcgcaaga agggtatacg
    37081 gtggcggtta attatcagca aaacctccac gcggcgcagg aagtgatgaa cttaataacg
    37141 caagccggtg gcaaagcatt cgtgctccag gcggatatca gcgacgaaaa ccaggtcgtt
    37201 gcgatgttta cagcaatcga tcagcacgat gaaccgctag cagcgctggt caataacgcc
    37261 gggatcttgt ttacccagtg caccgttgaa aaccttaccg cagagcgaat caaccgagta
    37321 ctttccacca acgtgacggg atattttctc tgctgccgcg aggcggtaaa acgcatggcg
    37381 cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct cttcggtggc ctcacggttg
    37441 ggttcgccag gggaatatgt tgattacgcg gcatcgaaag gggcgattga tacgttaacc
    37501 accggactat cgctggaagt cgccgcgcag gggatccgcg ttaactgcgt gcggccaggg
    37561 tttatttata ccgaaatgca cgccagcggc ggcgagcctg gacgcgtcga tcgcgttaag
    37621 tcgaacatcc ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc cattgtctgg
    37681 ctactaagtg ataaagcctc ttacgtcacg ggaagtttta tcgatttggc gggcgggaaa
    37741 taaaacaggg aagttgtctg accggatgca acaagtattg catccggtac ttcatcgact
    37801 taaagcttct cgccgttgct ggcaatcacc tcttggtacc agttaaagct cttcttacgt
    37861 gaacgcgaca tatcgccagc accgtcgtca tgtttattca catagataaa gccgtagcgt
    37921 ttgctgtact gcccggtggt gaacgatacg cagtcgatgc agccccacgg tgtgtagccc
    37981 atcagatcca cgccatcgta agtcaccgct tttttcatct cttcgatatg ggcgcgcagg
    38041 tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc gtaagcgcca
    38101 aaaccgtttt cgacaataaa cagcggcctc tgataacgct catacagttc gcaaagggca
    38161 tagcgcagac ctactggatc aatctgccag ccccagtcag acgctttaac atacgggttc
    38221 ggtacgctgc cttcgaaacc agagatcgca tcgccggtgc cgccttcggc cttcactgcg
    38281 ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcacgcag tacatcgaga
    38341 tcgccgtctt ccattttgat gttaaatccg cgacgctccc actcgttcaa cacataggac
    38401 gggtaatagc cgcgtagctg cacatcggta aagacgtagc gttcgcgcat cgactcctga
    38461 gcgaacatca catcgtccgg gttacagcag tagggataga gcggcaccat cgccagcata
    38521 cagccgactt tcatctccgg gtgaatgcga cgcgcagctt tcaccgccag ggcgctggcg
    38581 acaaactggt gatgcagcac ctgatacatc gtctcttccg ggttctcatg ctcggtataa
    38641 accacgccgg agcagcagta accgaacagc ggtgcacgcc agttacgctg gttgttaatt
    38701 tcgttgaagg tcatccagta tttgacttta tgcttatagc gttcaaacac cacttccgcg
    38761 aaacggacaa agaaatcaac cactttacgg ttggtccagc taccgtattg ctgcaccaga
    38821 tgcagcggca tttcaaagtg ggagagggtg atcaccggtt cgatgttgta tttcagcagt
    38881 tcatcgaaca tatcatcgta gaacttcagc ccttcttcgt ttggctgagc ttcatcacct
    38941 tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc catttcggca
    39001 aatagcttga tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg gtttggatag
    39061 tatttgcctg gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc gccaccggtc
    39121 agaacgtcac aaatgctcgg cccttttccg cctttgttcc agccgccttc gacctgatga
    39181 gcggcaactg cgccgcccca taagaaatct ttcggtaagg tgagtttttt cactatcata
    39241 ggctccagat gagtcaattc ttacaggaaa tagtaccatg cagccaaacg acatcacttt
    39301 ttttcaacgt ttccaggatg acattctggc tgggcgtaaa accatcacca tccgcgacga
    39361 gtctgaatcg cacttcaaaa cgggtgatgt gcttcgtgtc ggacgttttg aagatgacgg
    39421 ttatttttgc acgattgaag tcaccgcaac ctcaaccgta acgctggata cgctgactga
    39481 aaaacatgca gagcaggaaa atatgaccct gactgaactg ataaaggtca ttgccgacat
    39541 ctatcccggt cagacacagt tttatgtgat tgaatttaaa tgtctttaaa ttgaactcgc
    39601 gcctgttagt tgaaatttcg catttaattt catgattttt aggagattag tctcaaatca
    39661 gaatttattt tagctaacag gtgttcactg gaactattct cagttacgct ggagaggtat
    39721 acatgtgcgt gtacgtttct ccggagtaag ttatggttca gaagcccctc attaagcagg
    39781 gatattcgct ggcagaggaa attgccaaca gcgtcagtca cggcattggg ttggtgtttg
    39841 gtatcgttgg gctggtgttg ctactggttc aggcggtgga tctcaatgcc agcgccactg
    39901 cgataaccag ttacagcctc tatggcggca gtatgatcct gctgttcctc gcttcgacgc
    39961 tctatcacgc cattcctcat caacgggcaa aaatgtggct gaagaaattt gaccattgcg
    40021 ctatttacct gttgattgcc ggaacctaca cgccgttttt gctggtgggg ctggattctc
    40081 cgttagcgcg cgggttgatg attgttatct ggagcctggc attgctgggt attctgttta
    40141 aactgaccat cgcgcaccga ttcaaaattt tatctctggt gacctatctg gcgatgggct
    40201 ggctgtcgct ggtggtaatt tatgaaatgg cagttaagct cgcggcgggc agcgttacct
    40261 tactggcggt aggcggcgtg gtttattcgc tcggggtgat tttctacgtc tgcaaacgca
    40321 ttccatacaa ccatgccatc tggcacggct tcgtgctcgg cggtagtgtg tgccactttc
    40381 tggcgatcta tttgtatatt gggcaggcgt aatgggtatg cctttgtagg cctgataaga
    40441 cgcgccagcg tcgcatcagg catcggagca caactgccgg attcggcgtg aacgccttat
    40501 ccgatctacg ggcctcgtcc ctgttgtgat aaaggcatca aatcccagac atgataagac
    40561 gcgtcagcgt cgcatcaggc gataaatgcc tgatgcgata aagagattta ctcttcgagc
    40621 gaatacggca gcggctcgat atgcaatgta ttcgcatcgt cgcgtacgcg gaagatgcta
    40681 tccggttcca tatcgttatt catgacgacc tgtaccacga cctgaccatc ttccagtttt
    40741 accgcagcca gcaccgtacc ggtacgacgc cagttctcgc ccattttcag ctctaagtct
    40801 tcaccagctt ccggcagtcg gctggcgcta cctgccagca accagagcgc acgtttattg
    40861 gcaccacgga attttgctcg cgccaccatc tcttgtccgg tataacagcc tttcttaaag
    40921 ctgataccgc ccagcgcctg gagattggtc gcctgtggga taaactgccc gctgttggcg
    40981 gcatcaatca ccgggaaacc cgcttcaatg tttaatgcca gccactgttg gctattgttc
    41041 agttccgctt caccgcgcag tttatcggtc agcatattag cagtagcttc atcggttacg
    41101 atcaggaaac gttctgccgg gtgttcaaac catagcaaag tggtcgcgcc ttctttgact
    41161 acctgttttt ctttcgaagg cagttcgcta aagagatttg ccagcgcggc gcgcgcctga
    41221 aaaccggcaa caccaagcag cacacgctcg tcgtctggcg cgatggtcac tttagagaat
    41281 accgcatatt ttttcagttc agtcagctgc ggttcacgca cgctgcgccg ttcaatccat
    41341 gcaaagccat cgccgtcgcg gaacagacgt aaattgctcc acattttacc tttggcgtcg
    41401 caatgggcgg cgagcaggtg ctgattttct gtcatctggc tgacatctgc tgtcacctga
    41461 ccctgcatat atttttcgct gtccgcgcca gtaatggtgg caagcgccca gtcatcaagc
    41521 gtcatcagcg tcagcggtaa acgggcagaa gccgtaggct gacggggagg aaaaggtgta
    41581 aaagccataa tagagtcctg agttgcttaa cggcgtatta atgactaatg gtaaaagagc
    41641 tatgaggcaa tgcaagtgct ttcactacgc gatctgtgcc ttctgcgagg cgacttccaa
    41701 aaaaatgaag ccattgaatt gttgtgaatt aatttcgcga tcgcgatggt gtttactgat
    41761 attcccgtaa aagaaggcga aaaacacgtt acaatagcgg ggtactggat tttcgtgaga
    41821 caggaagaag atggacatta acaacaaagc ccgcattcat tgggcatgcc gccgtggtat
    41881 gcgcgaactc gatatttcaa tcatgccgtt tttcgaacat gagtacgaca gcttaagcga
    41941 tgacgaaaaa cgcatcttta ttcgtctgct ggaatgtgac gatctggacc tgtttaactg
    42001 gctgatgaat cacggtaaac cggccgatgc agaactggaa atgatggtcc gactcatcca
    42061 gacacggaac cgggaacgtg gtcctgtggc aatctgattt gcgcgtctcc tggcgcgcac
    42121 agtggctttc cttgctgatt catgggctgg ttgccgctgt tattttactc atgccctggc
    42181 cactcagtta caccccgtta tggatggtgt tactttcgct ggtggtgttt gattgcgttc
    42241 gcagccagcg gcgtattaat gctcgccagg gggaaattcg cttgttgatg gacgggcgtt
    42301 tgcgttggca agggcaggag tggagcatcg tcaaagcacc gtggatgatt aagagcggca
    42361 tgatgctgcg tttacgttct gatggcggta aacggcaaca tttatggctg gcagccgaca
    42421 gcatggacga agctgaatgg cgggatttac ggcggatttt gttgcaacaa gagacgcaaa
    42481 gataaagatg acggcaaagc atatgcgcta agccgtcagt gagatcaggc gtaatgctct
    42541 gccatttcgt tgaggatttg ctcgcaccag ctctgaatac gctcgtcgct aaggtcatac
    42601 tggttagttt catccagcgc cagacccacg aacagttgcc cgtcagcaat caccggtttc
    42661 gggctggtaa attcatatcc ttccgttggc cagtagccga cgaacttcac gcctttggtc
    42721 gagagtttgt catgcagcat accgagcgca tcgaggaacc actcgccgta tcccagttga
    42781 tcgccaagcc catacagcgc aacaatttta ccttcaaggt tcaggtcgtc gagctgatcc
    42841 cagacggctt cccagtcttc ctggatttca ccaaaatccc aggtcgggat acccagaatg
    42901 agcacatcgt actgctccat taatttcggg gagtcgtcct tgaggttatg taaggtcacc
    42961 agttctgggc cgataatatc gcggattttt tctgccgcca tttcggtgta acaggtgctg
    43021 gaaccgtaaa aaagacccat attcataacg taagcttctc gattctgctg tggcttttgc
    43081 agctagtgta tcagaatcgc tttagtcacg gcacaataag gcataatgga tcaaatgagt
    43141 gaccataagg ggcgcaagtg aaacaggatc tggcacgcat cgagcagttt cttgatgctc
    43201 tgtggctgga aaaaaatctg gctgaaaata cgttgaacgc ttaccgtcgc gatctgtcaa
    43261 tgatggtgga gtggttgcat caccgcgggt tgacgctggc gacggcgcaa agtgacgatt
    43321 tgcaggcatt actggcagaa cggctggagg gcgggtataa agccaccagc tcagcgcgtt
    43381 tgctgagtgc agtgcgccga ttgttccagt atctttatcg cgaaaagttt cgtgaagacg
    43441 atcccagtgc gcatctcgct tcaccgaaat tgccccagcg tttgccaaaa gatttaagcg
    43501 aagcgcaggt cgaacgttta ttacaggcac cattaattga tcagccactg gagctacgcg
    43561 ataaagccat gcttgaagtg ttgtatgcta ccgggctgcg tgtctctgaa ctggtcggac
    43621 tgacaatgag tgatatcagc ctgcgtcagg gcgtagtgcg ggtcattggt aaaggcaaca
    43681 aagagcgcct ggtaccgtta ggtgaagagg cggtttactg gctggaaacc tatctggaac
    43741 atgggcgtcc gtggctgctg aatggtgtgt caattgacgt gttgtttccc agccagcgtg
    43801 cgcagcagat gacgcgacag accttctggc accgcattaa acattatgct gtgctggcgg
    43861 gtatcgacag cgaaaagctg tcaccgcatg tgttgcgtca cgcttttgcc actcatttat
    43921 taaatcatgg tgcggattta cgcgtggtgc agatgctact gggccacagc gatctttcca
    43981 ccacgcaaat ttatacgcat gtcgctaccg agcgtctgcg gcaacttcat caacagcatc
    44041 acccgcgggc gtgatgtctg aaaagaacgg gaagatttat gaagaaaggt tttatgttgt
    44101 ttactttgtt agcggcgttt tcaggttttg ctcaggctga tgacgcggca attcaacaaa
    44161 cgttagccaa aatgggcatc aaaagcagcg atattcagcc cgcgcccgta gctggcatga
    44221 agacagttct gactaacagc ggcgtgttgt acatcaccga tgatggtaaa catatcattc
    44281 aggggccaat gtatgacgtt agcggcacgg ctccggtcaa tgtcaccaat aagatgctgt
    44341 taaagcagtt gaatgcgctt gaaaaagaga tgatcgttta taaagcgccg caggaaaaac
    44401 acgtcatcac cgtgtttact gatattacct gtggttactg ccacaaactg catgagcaaa
    44461 tggcagacta taacgcgctg gggatcaccg tgcgttatct tgctttcccg cgccaggggc
    44521 tggacagcga tgcagagaaa gaaatgaaag ctatctggtg tgcgaaagat aaaaacaaag
    44581 cgtttgatga tgtgatggca ggtaaaagcg tcgcaccagc cagctgcgac gtggatattg
    44641 ccgaccatta cgcacttggc gtccagcttg gcgttagcgg tactccggca gttgtgctga
    44701 gcaatggcac acttgttccg ggttaccagc cgccgaaaga gatgaaagaa ttcctcgacg
    44761 aacaccaaaa aatgaccagc ggtaaataat tcgcgtgaaa caacagatac aacttcgtcg
    44821 ccgtgaagtc gatgaaacgg cagacttgcc cgctgaattg cctcccttgc tgcgccgttt
    44881 atacgccagc cggggcgtgc gcagtgcgca agaactggaa cgcagtgtta aaggtatgtt
    44941 gccctggcag caactgagcg gcgtcgaaaa ggccgttgag atcctttaca acgcttttcg
    45001 cgaaggaacg cggattattg tggtcggtga tttcgacgcc gacggcgcga ccagcacggc
    45061 tctaagcgtg ctggcgatgc gctcgcttgg ttgcagcaat atcgactacc tggtaccaaa
    45121 ccgtttcgaa gacggttacg gcttaagccc ggaagtggtc gatcaggccc atgcccgtgg
    45181 cgcgcagtta attgtcacgg tggataacgg tatttcctcc catgcggggg ttgtacacgc
    45241 tcgctcgttg ggcattccgg ttattgttac cgatcaccat ttgccaggcg aaacattacc
    45301 cgcggcggaa gcgatcatta accctaactt gcgcgactgt aatttcccgt cgaaatcact
    45361 ggcaggcgtg ggtgtggcgt tttatctgat gctggcgctg cgcacctttt tgcgcgatca
    45421 gggctggttt gatgagcgtg gcatcgcaat tcctaacctg gcagaactgc tggatctggt
    45481 cgcgctggga acagtggcgg acgtcgtgcc gctggacgct aataatcgca ttctgacctg
    45541 gcaggggatg agtcgcatcc gtgccggaaa gtgccgtccg gggattaaag cgctgctgga
    45601 agtggcaaat cgcgatgcgc aaaaactcgc cgccagcgat ttaggttttg cgctggggcc
    45661 acgtctcaat gctgccgggc gactggacga tatgtccgtc ggtgtggcgc tgttgctgtg
    45721 cgacaacatc ggcgaagcgc gcgtgctggc aaatgaactc gatgcgctaa accagacgcg
    45781 aaaagagatc gaacaaggaa tgcaagttga agccctgacc ctgtgcgaga aactggagcg
    45841 aagtcgcgac acgctacccg gcgggctggc aatgtatcac cccgaatggc atcagggcgt
    45901 tgtcggtatt ctggcttcgc gcatcaaaga gcgttttcac cgtccggtta tcgcctttgc
    45961 gccagcaggt gatggtacgc tgaaaggttc aggtcgctcc attcaggggc tgcatatgcg
    46021 tgatgcactg gagcgattag acacactcta ccctggcatg atactgaagt ttggcggtca
    46081 tgcgatggcg gcgggtttgt cgctggaaga ggataaattc gaactctttc aacaacggtt
    46141 tggcgaactg gttacagagt ggctggaccc ttcgctattg caaggcgaag tggtgtcaga
    46201 cggtccgtta agcccggccg aaatgaccat ggaagtggcg cagttgctgc gcgatgctgg
    46261 cccgtggggg cagatgttcc cggagccgct gtttgacggt catttccgtc tgctgcaaca
    46321 gcggctggtg ggcgaacgtc atttgaaggt gatggtcgaa ccggtcggcg gcggtccact
    46381 gctggatggt attgctttta atgtcgatac cgccctctgg ccggataacg gcgtgcgtga
    46441 agtgcaactg gcttataagc tcgatatcaa cgagtttcgc ggcaaccgca gcctgcaaat
    46501 tatcatcgac aatatctggc caatttagcg tcatcttctc tataaaaaag agcgtggatt
    46561 gggtacaatc ccgctcttat caccgcattt tgactagctc aataaaagaa atcagaccat
    46621 gtttgaaatt aatccggtaa ataatcgcat tcaggacctc acggaacgct ccgacgttct
    46681 tagggggtat ctttgactac gacgccaaga aagagcgtct ggaagaagta aacgccgagc
    46741 tggaacagcc ggatgtctgg aacgaacccg aacgcgcaca ggcgctgggt aaagagcgtt
    46801 cctccctcga agccgttgtc gacaccctcg accaaatgaa acaggggctg gaagatgttt
    46861 ctggtctgct ggaactggct gtagaagctg acgacgaaga aacctttaac gaagccgttg
    46921 ctgaactcga cgccctggaa gaaaaactgg cgcagcttga gttccgccgt atgttctctg
    46981 gcgaatatga cagcgccgac tgctacctcg atattcaggc ggggtctggc ggtacggaag
    47041 cacaggactg ggcgagcatg cttgagcgta tgtatctgcg ctgggctgaa tcgcgtggtt
    47101 tcaaaactga aatcatcgaa gagtcggaag gtgaagtggc gggtattaaa tccgtgacga
    47161 tcaaaatctc cggcgattac gcttacggct ggctgcgtac agaaactggc gttcaccgcc
    47221 tggtgcgtaa aagcccgttt gactccggcg gtcgtcgcca cacatcgttc agctctgcgt
    47281 ttgtttaccc ggaagttgat gatgatattg atatcgaaat caacccggcg gatctgcgca
    47341 ttgacgttta tcgcgcgtcc ggcgcgggcg gtcagcacgt taaccgtacc gaatctgcgg
    47401 tgcgtattac ccacatccca accgggatcg tgacccaatg ccagaacgac cgttcccagc
    47461 acaagaacaa agaccaggcc atgaagcaga tgaaagcgaa gctttatgaa ctggagatgc
    47521 agaagaaaaa tgccgagaaa caggcgatgg aagataacaa atccgacatc ggctggggca
    47581 gccagattcg ttcttatgtc cttgatgact cccgcattaa agatctgcgt accggggtag
    47641 aaacccgcaa cacgcaggcc gtgctggacg gcagcctgga tcaatttatc gaagcaagtt
    47701 tgaaagcagg gttatgagga accaacatgt ctgaacaaca cgcacagggc gctgacgcgg
    47761 tagtcgatct taacaatgaa ctgaaaacgc gtcgtgagaa gctggcgaac ctgcgcgagc
    47821 agggggttgc cttcccgaac gatttccgtc gcgatcatac ctctgaccaa ttgcacgcag
    47881 aattcgacgg taaagagaac gaagaactgg aagcgctgaa catcgaagtc gccgttgctg
    47941 gccgcatgat gacccgccgt attatgggta aagcgtcttt cgttaccctg caggatgtgg
    48001 gtggccgcat tcagctgtac gttgcccgtg acgatctgcc ggaaggcgtt tacaacgagc
    48061 agttcaaaaa atgggacctc ggcgacatcc tcggcgcgaa aggtaaactg ttcaaaacca
    48121 aaaccggcga actgtctatc cactgcaccg agctgcgtct gctgaccaaa gcactgcgtc
    48181 cgctgccgga taaattccac ggcttgcagg atcaggaagc gcgctatcgt cagcgttatt
    48241 tggatctcat ctctaacgat gaatcccgta acacctttaa agtacgctcg cagatcctct
    48301 ctggtattcg ccagttcatg gtgaaccgcg gctttatgga agttgaaacg ccgatgatgc
    48361 aggtgatccc aggcggtgcc gctgcgcgtc cgtttatcac ccaccataac gcgctggatc
    48421 tcgacatgta cctgcgtatc gcgccggaac tgtacctcaa gcgtctggtg gtcggtggct
    48481 tcgagcgtgt attcgaaatc aaccgtaact tccgtaacga aggtatttcc gtacgtcata
    48541 acccagagtt caccatgatg gaactctaca tggcttacgc agattacaaa gatctgatcg
    48601 agctgaccga atcgctgttc cgtactctgg cacaggatat tctcggtaag acggaagtga
    48661 cctacggcga cgtgacgctg gacttcggta aaccgttcga aaaactgacc atgcgtgaag
    48721 cgatcaagaa atatcgcccg gaaaccgaca tggcggatct ggataacttc gactctgcga
    48781 aagcgattgc tgaatctatc ggtatccacg ttgagaagag ctggggtctg ggccgtatcg
    48841 ttaccgagat cttcgaagaa gtggcagaag cacatctgat tcagccgacc ttcattactg
    48901 aatatccggc agaagtttct ccgctggcgc gtcgtaacga cattaacccg gaaatcacag
    48961 accgctttga gttcttcatc ggtggtcgtg aaatcggtaa cggctttagc gagctgaatg
    49021 acgcggaaga tcaggcgcaa cgcttcctgg atcaggttgc cgcgaaagac gcaggtgacg
    49081 acgaagcgat gttctacgat gaagattacg ttaccgcact ggaacatggc ttaccgccga
    49141 cagcaggtct ggggattggc atcgaccgta tggtaatgct gttcaccaac agccatacca
    49201 tccgcgacgt tattctgttc ccggcgatgc gtccggtaaa ataagcatta cgttatgctc
    49261 acaaccccgg caaatgtcgg ggttttttaa ttgtgctgcg cgaaagcaga caacaatttt
    49321 cttgcttcac tattggctgc ctgcatcacc atccacggac tgaacgccca cggtgtggca
    49381 tcaataccgt gtaatacatc tgctaaatca caccattgat aatccatcac ttcatcatcg
    49441 ttgatctgta acgcactggt ggtgcgtgcg gcaaataccg gacacacttc attttccaca
    49501 atgccattcg gatcggtggc gcgatagcga aagtcaggat agatagattc aggaggcgta
    49561 atttccacgc caagcccata acggcaacgg cggatcaccg cgtcttcgtt gctttctccc
    49621 agttgtgggt gcccacaaac cgagttagtc cacacgccag gccacgcttt tttgctcagt
    49681 gcgcggcggg taactaataa ttgtcctttg gcattaaaca gccaactgga gaacgcgcga
    49741 tgtaagcggg tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt gggaactccc
    49801 tgtgcattca ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca
    49861 ctcatcatta tagataattg aagagtgaaa tgattaacca agtgagcgtt tatcgacaac
    49921 cgcccgtttt gagcggatgc cgacaggtaa aaaccattta aaaaagtagc atcatatatt
    49981 tgaagcgact ttcaatggcc attgcaacac agtcagaaat gacaggaata gagagagtaa
    50041 aaagcaacaa cagactgaat atagcgcgca ttaaaaaatt catattattc tccatgctca
    50101 tttttcttca gcttgcgcat cagaagccac atacgtactg tcctgacttt taaatcttta
    50161 tcagagacgg attccttcac caggtcagca tgatgctgat agccattaaa gaagacattc
    50221 agcacaacgg cgctaagggt tgcgagcata atgccgctat gcagcaacgg ttgcagtacg
    50281 gccggtaact tagaaaagaa atcgtgagag agcgtcggag tcatgccaac gccgagactg
    50341 atcgccacaa tatagaggtt gtaacggttg gtggtgtagt tacagcgcga cagaattcga
    50401 atccctgtcg ccagtaccat gccgaacatc accagcccag cgccgcccag cacaaattgc
    50461 ggaatggagg ctaccagcac cgccattttt ggcaccatgc caaacaggat taatataatt
    50521 cccgaagaaa tacacaccca gcggctatga acgcgcgtca cgctaaccag gccaacgttt
    50581 tgagaaaatg acgtgtgggg gaagctgtta aacgtgccgc ctatcattgt ccctacgcca
    50641 tcgacacgca gcccgcgaat aatatcgtgt gaagagagtt tacgaccgac tatttcaccc
    50701 agtgccagga acatccccat cgactcgata aacacgatga ttaacacggc agtcatggtc
    50761 agaatggaaa cgggatcgaa aatcggcata ccaaacgaca tcggggtgac aatcgcaaac
    50821 cacgaggcat catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag
    50881 ccaaatacaa tccccagtaa tacggcgacg ttggacataa atcctttcgc atagcgagta
    50941 atgagcaaga taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat
    51001 tgcggattcc ctttacctcc ggcggcccag tcaataccca cctgaatgat gctaagccca
    51061 atagaagtaa taaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccaata
    51121 agtggcgcta ataatgtggt gataaaaccc gcggctatag tggcaccaaa tatccccagc
    51181 aggccgatat ccgggttcat cccaatggct atcattggtg ttacagcagc aaaggtcacc
    51241 gacataatca ccggcaggtg gatccccata aagcggccga taccgataca ttgcaataat
    51301 gtgacgatcc cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc ttcttttgag
    51361 aggcccagtc ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg
    51421 acgtgttgca gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca
    51481 gttgggcggt cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt
    51541 tctcctttcg cccgccagag taaaaagcaa ccgtttacgt taatgaaact gtctaaacac
    51601 atgctcgtgg agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc
    51661 aatcacataa tcgtggttaa accacgaaaa tgagaagaga acctgacaat gaaattatct
    51721 gtaattttct catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa
    51781 tcgtttttat ctggatagcg ctcttttggt cggcaatgtt gaaataatga aagcggcata
    51841 gattgagggg caataatttg aactcagtcg caaaattata attaaaacgg tcatgctacc
    51901 taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat taaatgaaag
    51961 ggatgcaaat gaataagttt atcgctgctg aagctgcgga atgtataggc tgtcatgctt
    52021 gtgaaattgc ctgtgcggtg gcacacaatc aagaaaactg gccgctgagt cacagtgact
    52081 ttcgaccgcg tatccacgtt gtagggaaag gccaggctgc gaatccggtg gcctgccatc
    52141 actgcaacaa tgccccttgc gttacggctt gtccggttaa tgctctgact ttccagtccg
    52201 atagcgtaca actggacgag caaaaatgta ttggctgtaa aagatgcgca atcgcttgcc
    52261 cctttggcgt cgttgagatg gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc
    52321 gcagttccgg cacgcaagcc tgtattgaag tctgcccaac acaggcgtta cgactgatgg
    52381 acgataaagg gttacagtgg ataaaggtgg ctcgccagcg caaaacggca gcaggaaaag
    52441 cgtcatcaga tgctcagcca tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag
    52501 gcgcagataa aatttcagcg agtgaacgga aaacccactt tggcgaaata tattgcgggc
    52561 tggatccact gcaagcgact tatgagagtg accgctgtgt ttattgtgcc gaaaaagcta
    52621 actgcaactg gcattgcccg ctgcataacg ctattccgga ttacatccgt ctggtacagg
    52681 aaggaaagat tattgaagcg gcagaacttt gccaccagac cagttcctta ccagaaattt
    52741 gcggcagggt atgtccacag gaccgtcttt gtgaaggcgc atgtactttg aaagatcact
    52801 cgggcgcagt ctctatcggt aatctggaac gctacatcac cgataccgcg ctggcgatgg
    52861 gctggcgtcc cgatgtcagc aaagttgttc cccgtagcga aaaagtggcg gtgattggcg
    52921 ctgggccagc agggttaggg tgtgctgata ttctggcgcg cgcgggagtt caggttgatg
    52981 tctttgatcg ccatccagaa attggcggta tgctgacttt tggcattcct cctttcaaac
    53041 tcgataaaac ggtattaagc cagcggcgag agatattcac cgcaatggga atcgactttc
    53101 atcttaactg tgaaattggc cgcgatatta cctttagcga tttaacttct gaatatgatg
    53161 cagttttcat cggcgtgggg acttacggga tgatgcgagc agatctgccg catgaagatg
    53221 cgcccggtgt cattcaggct ctaccgttcc tgactgccca tacccgccag ctcatgggat
    53281 tgccggagtc tgaagagtat ccgctgacgg acgtggaagg taagcgagtc gtggtattgg
    53341 gcggtggcga tacgacaatg gattgtttgc ggacttccat ccgcctcaat gccgccagcg
    53401 tgacctgcgc gtatcgtcgt gatgaagtca gtatgccggg ctcgcgcaaa gaggtggtta
    53461 atgcgcgcga ggaaggtgtc gagtttcagt tcaatgttca accgcaatat atcgcttgtg
    53521 acgaagatgg gcgtttaact gcggtgggcc tgattcgtac cgccatgggt gagccggggc
    53581 cggatggtcg ccgtcgtcct cgtccggtag cgggttcaga gtttgaattg cccgccgatg
    53641 ttctgattat ggcctttggt ttccaggcgc atgccatgcc gtggttgcag ggcagcggaa
    53701 ttaaactcga taaatggggc ctgattcaaa ccggtgacgt cgggtattta cctacccaga
    53761 cgcatctgaa aaaagtcttt gctggtggtg atgcagttca tggcgcggat ctggttgtca
    53821 ctgcaatggc cgcaggaagg caggcggcgc gcgatatgtt aactctgttt gatacgaagg
    53881 catcgtgatg aaatcgttaa ttatcgttaa tccggctgac tgtattggct gccgtacttg
    53941 tgaagtagcc tgtgtggtcg ctcacccttc agaacaggag ttgaatgccg atgtcttttt
    54001 accccggctg aaggtacagc gactggacag catcagtgcg ccggtgatgt gccatcagtg
    54061 tgaaaacgcc ccttgtgttg gcgcttgccc cgtgggggcg ctgacgatgg gcgagcaggt
    54121 ggtgcagacc aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt
    54181 tggaatgatc accattcagt cgttgccggg ggatacccga caacaaattg tgaaatgcga
    54241 tctctgtgaa cagcgggaag aggggccagc ctgtgttgaa tcttgcccga cacaggcgct
    54301 gcagttgctt actgaaagag aactcaggcg agtccgccag cagcgtattg ttgccagcgg
    54361 tgagaatcct ctgtgaaaga aatctccgcg ctgaaaggcg cggagaatct attagattgc
    54421 ccaaccaccc gcgtaaaatg cgaccagtgc ggcagtaatg atcaccgtac caatattcaa
    54481 cttttgccat tcgcgagcaa agacacggcc tacgaccagt gtcacaaagc ccagcataat
    54541 accggtaacg atattacaag tcagaacgat gaacacggca cacaccaggc cagccattgc
    54601 gtcaataaaa tcgttgaaat ccagcttcga gacgttactt aacatcagca aacccacgta
    54661 catcagagcg ggtgcagtgg cgtaaccagg gatcaaaaat gacagcggtg ataagaacag
    54721 aatcagcagg aataacgccc ccactacggt tgcggtcaac cctgttttac ccccggcggc
    54781 ggttcctgcc gctgattcga tataaaccgc tgcgggcgct gcgcccacca gaccggagaa
    54841 tattgaactt actgagtcac tggtcagggc tttgccgcca ttgatgatct ggttgtcttt
    54901 atccaacaaa ttcgcctgac cggcgacggc acggatggtg ccagtggcgt cgaacactgc
    54961 ggtcatcacc aatgccagta cactcggaag tacggttggc tggagtgcgc ccataatatc
    55021 gaggctgaaa atcagagact taccatcttc gccagtcaga cttggcatcg ccaccagacc
    55081 gtggtatttc accgctgggt caaagattaa gccgataatc gaaattgcga taatcaccaa
    55141 caagatcccg ccgggtacgc ggcacttctc cagaccgaag atgaccgcca gccccagcaa
    55201 gctcatcatc accgggaagg aggtaaacgc accgagcgcc actggcaagc cttcaatcgg
    55261 gtttttgata accataccca caccgttagc agcaatcagc agcagaaaca ggccgatacc
    55321 gatacctgta ccgtgagcga tacccatcgg taaattacgt aagatccagg tacgcacacc
    55381 ggttacggaa atagcggtga agatgacgcc catcagaaag accgcgccca gtgcgacagg
    55441 aacgctaatt tgttgcccga gtaccagact gaatgcggta aatgccgtca aggaaatcgc
    55501 acaaccaatc gccattggca aattggccca caaccccatc agcaacgagc cgaagcccgc
    55561 gaccagacag gtggcaacaa acacagctgc gggaggaaaa cctgctttgc ccagcattcc
    55621 cggaacgacg ataacggaat aaaccatggc cagaaaggtc gttaagccag ccagtacttc
    55681 ctgacgaacg gtactgccac gagcggtaat tttaaaataa ttatccagcg cgccctgtgg
    55741 ctttggtgcg tccggtgttt gtaggatgtc tccagacata atgatgtcct cacgaggatg
    55801 ttaaagtttt attattagtt gcgttcgtac accagacgac catcaacgta ggtgcggtag
    55861 atcgaacggt catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa agaaacagag
    55921 ttgtcatagc gcagctgctg tagcggagtg gcggtgggtt ccatcaccac gaaatcagcc
    55981 tctttgccag gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt cgctccgccg
    56041 agcgtggcca ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa ttgcaatact
    56101 ttgtaggctt cgttcagcgt ttgcagcatg ttgaaagtgg ttccggcacc gatatccgtt
    56161 cccatgccca ctttaacttt cttctgccat gcttttttca agttgaataa gccgctgccg
    56221 aggtaaaggt tggaggtcgg acagaaagca atgctggatt tggtttcgct gagacgatcc
    56281 cactcttttt cttcgagatg gacgcagtga gcaaagacac agtttttacc ggtcaggccg
    56341 tactgatggt aaacatccag ataaccatca tggtcaggat aaagcgattt cacccaggca
    56401 atttcatctt tgttttcaca gagatgggta tgtacccacg tatccggata ttcttctttc
    56461 aggcgttgcg ccatcgccat ctgttcagga gatgaggtcg gggcgaagcg tggcgtaatc
    56521 gcatatagca gacgaccatt tttgtgccag cgttcaatca gttctttgct ttggtgatag
    56581 ctgctttcgg cagtgtcgag cagataatcc ggtgcgttgc ggtccatcat caccttaccg
    56641 gcaatcatac gcatattgat atgactggcg gcttcaaaca gcgcatcaac agattgcgga
    56701 tgaacagtgc caaacaccag cgccgtggtg gttccgttac gtaaaagctg cttgatgaag
    56761 aatgccgaca tttcgcgggc gtactctaaa tcctcataac gacgttcagt agggaaggtg
    56821 tgtttattca accactccag caattgttca ccataggccc ccaccatttc actttgcgga
    56881 taatggatat gtgtatcgac aaagcccggt actatcagtt tgccgcgata gtcgcgcacg
    56941 cgaatagtgt caggaatttg atgctttccg tcttcccatt cgccaaacca ttccactttt
    57001 ccctgtttaa tgagtaataa accatcctca ataaaccgca gcgcagaggc aatctcttcc
    57061 gggttatcga ccgtacgggt gacatcaata aaactgcctc gtaccgcttt caacgtgtgt
    57121 tctcctgaca tcattgactc ctttactgtt aatccatctc ttccgctgag gtaataccag
    57181 gcagaacgtt ttcctgtcgg tagccaccag ggaggataat atttaataaa atcgcagtta
    57241 acccgccagc acaaataggg ttttcaacta gtacataaat agaggctggc aatattttaa
    57301 aaatttcggg atcgtaggag acgccaagtc ctaaacctaa agaagtggcg acaataagtg
    57361 tttctcggcg ctttaaaccg ttggtgatga ttatgcgaat cccagcgatg gcaatcatgg
    57421 aaaacatcaa cgtcattgcg cctcccagaa ctgccgaggg aatggtcgtg aagaagccgc
    57481 caatcatcgg aaacaaaccg aggataacca gcattaccgc gatggttcgc ccgacataac
    57541 gtgaagcgac gccagtcatc tgaataaccc cattattttg cgcaaacgtg gttaagggta
    57601 atgacccgac agcggaggcg ataacagaaa ccagaccatc tgccagcacg ccgcctttca
    57661 gtcgggactg atactcttcc ccctgaatgg ggcggcggga aaccattgcc gtggcggtga
    57721 tatcgccgac tgcttccagc acgctaagca gataaatcgt gccaaccacc aggaactgat
    57781 ggaagctaaa actaaagccg tatttgaacg gatgcgggat ggtgattaac ggcaaattgc
    57841 gcatactgct gaaatccacc atgcccaggc ataacgatgc gatatagccg acacatagcc
    57901 caatggcgat ccctcccatg cgtagcaacg gactgcgaca gcagttaaag ccgatcacca
    57961 caattaaaac caataaacca acgccgagat gttcgtaatt gccgaacgta ccgctgcttt
    58021 tggctgcaaa tcctccacca aaatcgataa tgccgacttt aatcaggctt aagccgatca
    58081 tcagtaccac aataccgctg acggtaggcg taataacccg acgtaaatag ggcaagataa
    58141 atgaagatcc gacaaccaga aatgcgccaa cgaaggagac gccgagaagc gacgacatga
    58201 ttaactcttc gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc atgaccgtaa
    58261 caaatgaaaa attgactgac tgaattgaga gtaggccaga accgacgatg ccgtagcggt
    58321 ttacttgtaa ccaggtacca ataccagagg cgatcatcgc catagaaaca agataggcgg
    58381 ttgtttcagc ggaaagctgt aaggccgcac ccacgattaa cgcgggggtt accatcggaa
    58441 cgaaaattgc caacagatgg gtaatggcac caacgagagc ctgatgaaag ggagggcgat
    58501 cctccagttc aaatataagg tcagaacctg catggtttat atcagacatc ccttcactcc
    58561 ttttaaagta aatttgatca tctgaattag taaaataaat tcccacgaat ttggggcgca
    58621 ggacgccttg agaacggctt atctcatttt cgcagtcact atttagtcat cgttcaaaca
    58681 gagaaacagg ccgccgaagc ggccatttat tacttatatt ttttccagcg cggtgagtat
    58741 tttctccggt gtgaaatgcc attcgcgtaa ccagatgccg caggcatcgt gaattgcggt
    58801 agcaatcgcc ggagctgcgc catttacacc aatttccgag atcgatttcg ccccgaacgg
    58861 gccgacttta tcgtcgctcg gcaccaacac agcgcggaaa tcacgcggaa tgtcaccaat
    58921 tttcggtgcg ccgtaactgc gtaaatcacg cgttaacggg tgaccttcgg cgttataaat
    58981 gatctcttcg ctcatactgt ggccgatagc acgcagggtg gcaccgtaga tttgtcccaa
    59041 cgctaactct ggattgaccg gtgtaccgca gtccagcaag gcgtagaatt tatccaggcg
    59101 gatttcaccc gtacgcgtgt tgacggcaac ttcagcgaag tttgcgccat acgggaaagc
    59161 gaaatcaggc gtgatataac tgccagttcc caccagtgaa ccaaagccgg tgccggtttc
    59221 gcctttatgg gcaatatccc cgaaactaac ttcgcctttc ttgccgcgca cgacgcccgg
    59281 cgttgctagt tgaacatctg ccactggctc acccaacatt tgcgcgccgt ggaacagaat
    59341 tttctcccgc agattttccg ctgccaaacg cgccgcgtta cccgagaagc aagtaccgga
    59401 cgaggcatat gcgcctttat caaacaacgc atgatcggta tcaccggaga taacatgcac
    59461 gtcctgcggt gggcagtgca gcacttctgc tgccagtttc gtcactacag tatccagacc
    59521 agtaccaata tccgcaccgc cagaatgaac gataaaggtg ccgtccgatt ccagtttgat
    59581 catgcagtta gcctgatcga tatccgggat acccgatttc tgcatgataa tggcgacgcc
    59641 gcgaccgatg tgccagtcac tattttgcgg tttcggtgaa gaccattgga tcatctcgcg
    59701 accctgacgc aggatctctt ccagtgcgca gctggcggcg gaaggaacgg aggtcggcgc
    59761 tttaccttca ccgattgcac cgagaatttt cagctcttgc ccttcgtgta cccggttacg
    59821 ttcgataatt tccagttggt cgatctgtaa ctgttcagcc agttccgcta atgccatggt
    59881 gatagcgaag ttacctttcg gtgcgccata accctgataa gcaccatttg ggcaaatgtt
    59941 gctgtagtag gtggtgacct ggaaatcgac gttatcgcac ggatataacg gcagcgacag
    60001 cgccggtccg ttacacggta cggtgagtga gtggttgccg taagggccag tgttggcgcg
    60061 gaaatccatc ttcactgccg tcaggcgacc atcttttttc gctcccagtt tgacggtgac
    60121 tttcgcgacg tgacgagagg tgttagcaat aaactcttct tcacgggtgt agcggaacag
    60181 taccggacgc ccggtcacgc aggttgccca tgcgcacact tcttccagca ggatgtcctg
    60241 tttggagcca aaaccgccgc caactcgctc tttaatgaca tgaactttat gctgtttcat
    60301 gcctacgagg cgcgcgacct ggcggcgtaa gtgccatggt acctgggtgg aggcgtggat
    60361 aaccagacga tcgccgtcca tccgggtaaa gcagatatgt gtttcagtcg ggcactgctg
    60421 tgcctgcgtt gagttatagg ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc
    60481 catatcgcca atatgaccat gaatactggc ggcgatattt ttgcgagggc gagaaccgat
    60541 cgggaagttg atgatcatat gctcgccgcg ctgggctgca tggctgttat cgtcttccag
    60601 agtatctggc gcaccagcaa catacaccac cggttcatcg tgcacgacag gcgcatcttc
    60661 cgccattgct tcgtcgatcg acattaccgg cttaagcact tcatattcaa cgtcgatgag
    60721 cttcaatgct tcgagcgcaa tttcttcact ttctgcgacg accgcagcaa cacgatcgcc
    60781 gacgtgacgc attttcttgc cgaacatacg gcggtcaagc ggtgacggtt ccggtgcgct
    60841 ctgaccaccc ggggtatagt agatatccgg gcaattcagg tgagtaataa cgtgaacgac
    60901 gcccggtaag gcttcagctt tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg
    60961 gctacgtaac attttaatga cgcaggcgtc agccgttacg cggtcttcaa catagcaggg
    61021 tttcgcctgc accattttcg cggcatcagt tttaggataa tgcttgccaa tgacttcaag
    61081 gtcgtcacgg aaagtcggag cgatatcaat ggtggcctgc ggattatttt tacgtgcaac
    61141 cgccagttca atgacctgat agtattgttg ccagcctgca tcgcggctga acaaaccaga
    61201 aagcgcgtcg tcgatctctt cgcgggtagg tgcggcgatg cgatcgagaa gatcggtgat
    61261 aatcagagct gcagctggat cgttataacc agactgcacc acgccaacat caaccattgc
    61321 ctgttgaacc agacttaact cgttccattt gcccaaagat tctgcggtac gaatatctgc
    61381 cttctctaac tgtgcggcaa taagcaagga cgcgttaacg atattaccgt taaagattat
    61441 tgcgtcagaa ccggcaaacc cgaagccatc atcactgttg cgtacagagt gcattcccat
    61501 gttaaacaac agcttttgca cgttttcgcc tggattaacg gttagctcct gaggcgcgcc
    61561 atttaaagta aagtggatga tcatacggct tcctccccag cctgttggca gtcggcatac
    61621 aggtcggcta ccagaactcc cgtgatatag cgtttatagg ccacgctgcc ccgcaaatct
    61681 tcctgcggga aaatggcgtt ggcgacagcc tgttcaagtg cattgccttc cagattttgt
    61741 ttttcgacat catgcagacg cagtgcttta ctggcgacgc catccagcgc aattcgcata
    61801 ccgtcgtggt ctgtcattgc aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg
    61861 ctaattttgc gggtcgcaca ggtgcgatac ggatctttaa tgataatttc ggttagcagg
    61921 cgatcgcatg ggcaggccag gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc
    61981 agttcagcat ccagtgccaa caggacggga agcagcaccg actcttcctg gcgggcggcg
    62041 atttcaccac caatggtcga ctgattacga acatggcgtg agtaaacaaa accgagggct
    62101 tcacgcagcg ctgcaggaat aaatcgcgca tcacgcagtg gctgcaagcg agacattgcg
    62161 ccaatccgca gtgcaccgtt atcccagtca acccagtcca gttccagatc ctgtaaggaa
    62221 atggcaatct ttttatcggt acgggttggt gtagcattga gtttgctgcc cccggcgaac
    62281 cagacggctt catcctggta gcggcgcttc agttccagcg cctgttcgac agagtcgggc
    62341 ctgaaaaatt gttcaatcat cttctttcct tatttaaccc tcttccctgc gggaagaggg
    62401 catctgtcat ttatgccagc gcatccatcc gacgccacat actggcagcg gcttttctgg
    62461 cttgtgcata aatggaatcg caatcgaagt taaactgacg gtcttcatag accatcacac
    62521 cattgaccat cacgctgtga acgctgcctg agcccatacc gaaagcgata tgcccggcga
    62581 tattgtctgc cagcagcggc gtcggtgagt tgtaatcgca aatggttaaa tcagctttgt
    62641 aaccggcttc cagaagtcca aatttcgcgc caaagttgcg gctcatcagt tcgttaccgt
    62701 tagtcagggc tttggcaaaa ctgtcaggcc acagcggacc acccgcatcg cgatgtttaa
    62761 agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat gccgtccgtt cccaacgcca
    62821 gattgcggat gtcgctaagg tgatggttgt agccgacatg gttgttcatg tttgaacggg
    62881 cgttatgcac caggaacgca tcgcgctgat tgagtagggc gatgtcatct ttcgacaagt
    62941 acagcccatg agcgaccagc gttttgctat cgatgagatc gaattgcgct agtcgtgcca
    63001 gcaggtcttt gccgtaccag tggtgactgt aggaaacgtc gtaaaggtct tccgcagcgt
    63061 gaatatgcaa accacggcct gtggctttca cggcttcacg tagcatctcc agaccggcat
    63121 ctggcacggt aaacggcgcg tgagcaccga tatgtgcttc caccagatac ggctcggttg
    63181 ctgctttctt cgcctcatca atctgacggg cgaagcggat gttttcttct acaccttcct
    63241 gcaactcttt gatgccgttg ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc
    63301 caacttttaa aaatgcgtcg cgcaatgtgg agagcgaccc gccgatatac gccggagagg
    63361 catggtgatc gataaccgat gtacatccgc tcttaatcgc ttccagggaa caaatcagcc
    63421 cgctgtaata gagcgactct tcatcaaggg cgcgatcgag ccgccaccag agatttttca
    63481 gcgttgagat gaaatccggg cagggggcga tgtttgccat aattccgcgg gaaagccccg
    63541 agtaaaaatg gttgtgcgag cagacaattc ccggcatcac aatccgacca tgcatctctt
    63601 tgtagctggc atcggggtag cgttgcgtca gggcatcgcc gatagcgaca atcacatcat
    63661 tttcgatagc gatatcaacg ccttcctgca ctttcgccgg gtgtaactgc actgcggtga
    63721 cattcttcag aatcaacatg attacacctc cacgcggccc agcagataat gatgatgctg
    63781 gtggacatgg ctgatgatgc ggcacatatc gttcagctcc ggtggtacgt tgttgaactg
    63841 accttcgctg tcgatgttta acacccagct ttggttattc agtcgtactc gtacccggca
    63901 atcttccaca aggaagcctg ggttgctgct gttatcaaag tcttgcgcca ggctgaaaac
    63961 ggtgattttg tctttgtatg gtttaccgtt ccacggacag aactgagcgc agttgccgca
    64021 ttcgttacag taagcgtcga ggtgcagcgt ctggaatcta ttctggaagc ctgggaccgc
    64081 aatggagacg ttggcgcggt tcgggcagac atccacacac ttgctgcaaa cgtagttaca
    64141 ttcgaggcag cgagcggctt cctgggcgac aaacgcgtca cgatcgtcac tgttcaccag
    64201 agtgatagag atatcgcctt tacgttgata gatttccgcc ggattgacgt tgttccagta
    64261 tttatcgttc tggtgggaac ggatattttc ccgactaagg atggcatcgg tcgcccgacg
    64321 cgcggttccg acagcagcga caatggagga tggtccgcgc tgtacgtcgc cgatcataaa
    64381 gacgtcagtc agacgagttt cgccattgtg gtcgacgtct ggccagccgt ttttgtccag
    64441 cggcacgccc atcgcattca gggcttcagt atcctgctgt tcaccaatgg cggtgatcag
    64501 gctgtctaca tgcagtgtta ctgtttcgtt ggtttcaacc ggacggcgac ggcctttctc
    64561 atcgggttcg ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat cgaaacgttc
    64621 cggattattc aggaaacgga actccacgcc gtcgtgcaac gcttcttcat actcttcgcg
    64681 ccatgcgggc atctcttgca gtgaacgacg gtaaacgatc gttgcttttt ctacgcctgg
    64741 aacgcgtaac gccgcacgag cgcagtccat tgcggtgtta cccgccccga caacgaccac
    64801 atgtttgccc agcttgagcg ctgtaccctt gttgtattca cgcaggaagg ggagtgattt
    64861 ccaaacattt tggttgtcac ccgccagttt cacaccgcta tttttatcag tgccggtggc
    64921 aatcagaaca tagtggaagc cctgattttt taactgttca acggtcaaat cgggtgagca
    64981 gccatactca aatttcacgc cgtgatcggc aacaaaatcg atatcgtgct gaattaactc
    65041 agccggaatt cggaactgag gaatgatatt tttcaccacg ccgcccgcat tggcttcgcg
    65101 ttcaaacagc gtaaccggat ggcccgctct ggcaaggaag taacctgctg ccagacccgc
    65161 cggacctgca ccaatcacgg caaccggatg gcgtgaacca gaaccggctg gtttgtgcca
    65221 gcgttgctta tattcatccc aacctttttc cagcgcgact tttttcagtt cgcggatatt
    65281 cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg cactggtgat cgcaaatatg
    65341 accggtaatg gcgggcagag cgttgcgttg gtagatgagt tccagcgcgt cggcatagcg
    65401 gtgttcgcca agcagacgga tgtattccgg aatatcttgc ttgatagcgc aggcagtaac
    65461 acagggggca acgtagcaat cggtcagcgg caggtcttct gccacttcaa tacgctcttc
    65521 tggcttccag tgtttctggg tgtattccat ggttaacgca tctgctgcca gtctgttcag
    65581 tcgttcgacg tcaacatggt caagtcccca ggcgtcggag ccttccagct cgcgcatgca
    65641 ggcacttaag cgcaaatagc cgccaggttt cagcaggtcg gttgccatag taatagggcg
    65701 aatacctgta tcaaaaatat cgcggatagt cagctgactg gcaccaccgg aataagaaat
    65761 gggcagtttg ccgtcaaagg cgcgagagag aactgctgca acattgatgg agagcgggaa
    65821 cagcgcacgg cctgacatat acatctcttc accaggcagt gcgcctttat tgttgatggt
    65881 gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg ccagtgccat
    65941 caggcgttcc agcatttcca gtgcttgcgt cagcttgagg tcgtgatcaa atgactcttc
    66001 ttttaagcct atgtaaccga aaccgcagac atcgaggatc tcacgaacac gcgcgtatcc
    66061 cagtaaggtc gggttaagtt tcacaaaggt gttgagccct ttttcttcca gcatgtagcg
    66121 gcaaatggct tcgatttcat gcggaggaca gccgtgcatg gtggagaggg tgacgccctg
    66181 caccatactg gtggggatgc gagcgggtaa ggcttgcaag ctttcgcgtt tttcctgcaa
    66241 tccgtggcgg gctaaaaatg cgtcatcctg gagtaattta ttcagcgtat cgcgatattg
    66301 cgcgaatttc ggatggtcag atgcgtccat catattgtcg atgaactgtt gcattggcgg
    66361 ttgcttaata ccttcgaggt tgtaaccgac gctcatatta aagatgaacg atttaccgga
    66421 atcagaaggc tggaacatcg cttcgagaag gtgcagggca aaccaggctt tgaggtattc
    66481 atcccaggct ttaagcaggg taaattcggt agaccattcg gtgttaaagc actcgtcttc
    66541 ggcatcgata cagggctttt ccagctccag gcggtcaaga atttggacgg tttttagttc
    66601 gatgaagcgt ccgccagtca gccaggaagt gacaatattt tgcgcgagct gcgtgtgcgg
    66661 gccagcggca gggccgacgg gagtggcaca ggtttcaccg aagacgctaa cagttttacc
    66721 ttttacaggt gagtaaaact gttgctcggg aataccaaag attgagcgtt gttgttggta
    66781 ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg ggacgcataa tatcccccat
    66841 aaccctctcc ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt
    66901 ttagggggat agagcaacaa tcgcaccact cttgccaggt ttggcgggaa atataaaaaa
    66961 atgtgaggcg gctcgaaact taaaggcttt ttgtgaatga actcacaggt gccaaatgag
    67021 aattaaaatc actcctgaaa attattattg tcagcccatt tatcactttt tctcttttct
    67081 caaaatcagt gattccttat cattatgata aagagtgctg gagagattaa atatttgaac
    67141 gcaatcgttt aactacattt tctaaatttt agtatacctt ttttgcgctg taataaagtc
    67201 gtccggagta tcaatatcta aaattatttc tgcattttct atctcgacgg aataatgagc
    67261 tcccataagg agtgcctgac gcatattggt gacattgggt cgttggattg cctgcatcaa
    67321 gcatggtttt gacactaaaa tcggatggcc ggggatgcca ttatggagtg gcagtattgc
    67381 gccatcattt cgtaacgacc agatttttct aaaaatatcg atggtgaggg ttggcatgtc
    67441 accgtgggtg agaaaacaat gttctgtttg cactgcgggt actgcggcct ttactgatgt
    67501 tagtaaaccc tgcgcataat ctgggttgtg gataatagtg atattgctct ggttcgcata
    67561 acgttcgtgc agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg
    67621 caacgcattt ttgatacttg tatcaagaat tgtcccctgt tgccagggta acatcatttt
    67681 ccattgcccc attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat
    67741 agtggttcca ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga
    67801 ttaagttggg gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac
    67861 ccatcggcat tagtcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt
    67921 ggtgctgggg ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc
    67981 aggcgtgtgt taatcacgac gacgacacat atgtttatgc caacgtctca ctggcccgtg
    68041 gttttctgtc gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt
    68101 tgttttcaca gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt
    68161 gatgcactgg tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt
    68221 ggaatgccgt taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc
    68281 gtgattgctg tgatgggagg acatactttg ggcgcgaaag tgagcacaga aaatgtccat
    68341 cgctggtcgc agtttgctga tattaccggg ttaacacctg atgcaacctt gcaactgagc
    68401 gatctcgttg cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt
    68461 cggcgagtct ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag
    68521 ctccttcaac cgctgcaaca acacgacgta gaggcaatct ggctgggcga tatacaagaa
    68581 catcctgcaa tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa
    68641 ggatatccat tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa
    68701 attgtccgtt tgcgatggcg caaattgttg atagccgagg ctcgactccc cgccattctg
    68761 cacaaatgtt agtgcgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg
    68821 agcggaaggt gattgaagag tcacttcagg cattgcagga acgtaagccg cgattattcc
    68881 atggacgtat ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt
    68941 cagtatttat cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg
    69001 tcaaccgggc gatagcccag agtgcggcgc tattaggatt tgatattgcc gttgccgata
    69061 tttatcgcga aagcctcaat cctgaactat tccctccatc aaccacgctt ctccatgctg
    69121 agtcgtttgg tgcagcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg
    69181 ccacgaataa tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt
    69241 tggggttgct ggcaagtcgc cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag
    69301 gcgtggctga agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg
    69361 aaacgccgca ggagatcgcc atcagcgtgc tggcagaaat attacaggtg aaaaataacg
    69421 cgccgggtgg gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc
    69481 gcggtgcggg agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag
    69541 tgatcatgct ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg
    69601 ccgtgttcga tggcgaaatg acggtcgaag gcgttactgc tcgcctggca accagtactg
    69661 cggaagcgat gaaacttacc gaacgcggat tcatccctgt gatggcagat cccgcctgtt
    69721 cattgcttga tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt
    69781 tgggaacgcg ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgcag
    69841 ggaaggattg tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt
    69901 actctggttg tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc
    69961 gacgggtgat ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc
    70021 tggtgaaaga gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga
    70081 cggggatggt gcgtggcttg ttgaacgacg gcctggcagt ggtcggtggt tttaaaattg
    70141 gtgatatcga tcctcgtggt gaaacggctg atttcaccag cgtttctgat aaagcccggg
    70201 cgattggcgg cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga
    70261 caaaagaagt gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc
    70321 ttaaaattac tgacaaatca ccgttccggt tttgccttca atcccctctt tcgctttgct
    70381 caatacggta atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc
    70441 cactttaggc agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc
    70501 acttaatgac aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt
    70561 ttctaccgca gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct
    70621 ggcccagtct ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac
    70681 aggaatacca ccaccgccaa cggtgatcac cacctggcct gcatctacca gagctttaac
    70741 cgtttctttt tcaataatat caaccggttt tggcgaggca acaacacggc gatagccgcg
    70801 accagcatct tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt
    70861 aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc
    70921 gacctgagta accagcgttg ctaccggttt attaatgccc cgagaaagca gttcttctct
    70981 taaggcgttt tgtagatcgt aaccaatata accctggctt aaggcaacgc agacagacat
    71041 cggcagcatt ggcgagtgcg cttcagtttt tgcagcagct tcaaaggcct ggttaatcat
    71101 gcccacctgc ggcccgttac catgagtgac gataacttca tgtccttggg caattaaatc
    71161 aacaattgcc tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag
    71221 cgcattcccg cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt
    71281 gattgtgtat agatccctga cagtggttag agcacgggag ggacaaacgg tttgcgtcgc
    71341 aggaaacgac ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca
    71401 cgggataagg ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg
    71461 gcgttgtcgt ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc
    71521 acgtcgccat cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg
    71581 gcgggcattg cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg
    71641 ccactggaaa acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga
    71701 ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc
    71761 gccaccacgt caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg
    71821 ttacgcagcg gtgggctaag aatgaacttc atgccgtctt ctgtatcgta actgcgttcg
    71881 tccaacagga gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca
    71941 agacgcagat aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta
    72001 ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg
    72061 cgactcaagg catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg
    72121 ctggcgatag ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaga
    72181 cggcgtaatg cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt
    72241 ttaaaactgc tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta
    72301 atgtgctgga tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca
    72361 cgataaacct ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca
    72421 ataatggttg ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa
    72481 ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga catccacgcc gccagggaaa
    72541 acgtaacagc cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc
    72601 aactggcgaa caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta
    72661 acgacagtgc cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac
    72721 cggaggggaa taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc
    72781 gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc
    72841 ttcaggttct ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga
    72901 tacgccgtta gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc
    72961 gtaggcattc accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa
    73021 gtagcattcg gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga
    73081 aacaaccgcg ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc
    73141 ccaggtttcg ccccaggtca gacggcggtc aatagagacg gcgcagctat ctgctacagc
    73201 gcaacggctt ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa
    73261 ttcgtcataa cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt
    73321 gaaaatggcg ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac cctgaacatc
    73381 aatacgaatt tccatacgac cgcgctgacc acggtatatc tggcagtcgg ttggttcggt
    73441 actgaccaca aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc
    73501 gtcgcagtct tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc
    73561 gaggtcttta atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga
    73621 agtaccgcga ccaccgatta gctcatcagt ttccatgcct tcgtacggat cgaagtccca
    73681 gtttttgatg ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg
    73741 gccgtggccg atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc
    73801 gactttttcc atctcttctt taatacgatg tactacgcgt ttttcgtcgc agctttcact
    73861 gggaatagca accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt
    73921 ttcaagaatc agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc
    73981 cccgttttgc agggagtagt tccaatagag tgattaaggt gctacagcgt gtttgccttc
    74041 ccagacaact tcgcggtagt gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac
    74101 cacggcatct ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg
    74161 aaccgctgcg agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc
    74221 gttgccgtac ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga
    74281 gatgaattgg gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca
    74341 ggccaggcct gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac
    74401 gccggagcga taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg
    74461 cgggctatag acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc
    74521 ttgcagcaga acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc
    74581 tgccagggtt gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc
    74641 cgtgtcctgt accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt
    74701 atcgtcatag ttcatatctg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac
    74761 gcgttcctga gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgcgc
    74821 tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc
    74881 gccgatggca tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc
    74941 acacaataat tgagcgatag cgtacgcacc gccgagcatt ttgaaggcgt tcagaccgaa
    75001 tcgttttgat tcgtctttga caagaatttt cttcacccca aaaaggtttg cgagatcgtc
    75061 cagcgcacaa agcggtgttg ggcgataacc agctattttc tggtggaact ggcgcgccag
    75121 tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc
    75181 gatatcaatc ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag
    75241 ggccgaccag ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag
    75301 ttgctcgagc agtgcacctg gtttggcgta tttacgggac aggatcatcg cggcgatgat
    75361 ataaggcttc cagctggctt ctttgtaggt agcgatacgg tatttttcga atacgccttc
    75421 agtcacttcc ccttctttgc aggatacgcc gctgatatca gctggcaggc agtgcatgta
    75481 cagggcttcg ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg
    75541 ttgcgcgttc tgtgccagac actgtttttc cagtgctttt aagccttcgt gatcgttagc
    75601 gcgcagcaat tcggtacgct gttccatcac tttgtaaggt gcccatgact tcggataaac
    75661 gatgtctgca tctttgaacg cttcttccat gctggtgacc tgacggaagc taccgccaga
    75721 tgctttagca ttgtttttcg ccacttcaac cacatccggg atcaggtcgt agccttccgg
    75781 atgggccagg gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac
    75841 agagagcggt ttgccatagc ttggagagta ggcccaggtc atggcgattt ttttaccttt
    75901 caggttttcc agtgaaccaa agtgttcacg taaccacgcg aggtcagcca ttgactgagt
    75961 cgggtggtca atatcgcatt gcaggttcac taaagccgga cgctgtggca gtacaccctg
    76021 cttgtaaccg tcatcaagtg cagcgccaac ttcacgcata taggcgttgc ctgcgcccag
    76081 atacatatcg tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc
    76141 acgcacggtt tcgccgtgag cgatttgtga tttgccttcg tcgagatctt gttgtgcaag
    76201 gccgagcagg ttaagcgcgg aagcataaga gaagcgggta cgggtggagt tgtcgcggaa
    76261 tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc
    76321 ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt
    76381 taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc
    76441 attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagtg gtttatttca
    76501 ctgtgacttg ccaaagtgat tggtgttttc acagctatac ctgggactaa caataagtgt
    76561 gccagaatta tccacggggc tgcaaaagac taatttgacg gataatgtgt ggggttgatc
    76621 acaaattgat gccagcttcc ggttgtttgt aaaatacgaa aacgtttggc tttttttatc
    76681 gcgttattta tttgctgcta agtatcagaa ataagataaa gggacaggct atctctcttc
    76741 agtttgataa actcaccgcc gtaattctca tattgatata gaaatgtgat atctcttcca
    76801 taatttcatt agaaaatgat aaaaatctta tgtttttatc aaagccaatt tcgcctgagt
    76861 taatatcatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt aagtaagtct
    76921 ggaaggtaaa gctatggagc ttgctactac gcagtcagta ttgatgcaaa ttcaaccgac
    76981 aattcagcgt tttgccagaa tgcttgccag cgttttgcag cttgaggttg agatcgttga
    77041 tgaaaacttg tgtcgcgtcg ccggaacggg cgcgtatggg aagtttcttg gtcgccagtt
    77101 gagcggcaac tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa aagttgtgac
    77161 acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt gccgtgaaaa
    77221 agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga taagtttgat
    77281 tgccgttacc cacgagcaac aagagcatat cagtgataat ttacgcgaat tttctgatta
    77341 tgttcgccat atatccacca tttttgtttc gaaacttctg gaggatcagg ggccaggaga
    77401 taacatcagt aaaatattcg cgaccatgat cgataatatg gatcagggcg tattagttgt
    77461 tgatgatgaa agtcgggttc agtttgttaa tcagactgcc ttaaaaacac ttggtgttgt
    77521 acaaaataat attattggga aacctatccg tttcagacca ttaacatttg agagtaattt
    77581 tactcatgga catatgcagc atattgtttc gtgggacgat aaaagtgaat taatcattgg
    77641 tcaattgcat aacattcagg gccgacaatt atttttaatg gcatttcacc aatcgcatac
    77701 cagtttttct gtagcaaatg cacctgatga accacatatt gaacaattgg ttggcgagtg
    77761 ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc catccagcgt
    77821 tatggtggtt ggtgaaagcg gcacgggtaa agaagtcgtc gcccgagcaa tccataagtt
    77881 gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc cggagcagct
    77941 tctggaaagc gaactgttcg gttatgttaa aggcgcattt actggcgctt ctgccaacgg
    78001 taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg aaataggtga
    78061 tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcgcgtg aaattctacc
    78121 gattggtgcc agtagcccaa tacaagtcga cattcgcatc atttctgcaa ctaatcagaa
    78181 tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc gacttaatgt
    78241 tatcccgata actctgccac cgctgcgtga acgtcaggaa gatattgaac tattggtgca
    78301 ttacttttta catctgcata cccgtcgtct gggatcggtt tatcctggca ttgctcccga
    78361 tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac ctgcgcgagt taagcaattt
    78421 gatggaatat ctggttaacg tagttccttc aggtgaagtt atcgacagca cgctattgcc
    78481 gccaaatctg ctgaataatg gcacaacgga gcaaagtgat gtaacagagg tcagtgaggc
    78541 gcacctgtca ctcgatgatg cgggcggcac ggcgctggag gagatggaaa agcaaatgat
    78601 ccgcgaggcg ctttcacgtc ataacagcaa gaagcaagtt gctgatgaac tgggcatcgg
    78661 cattgctacg ctctatcgca agattaagaa atatgagttg ttaaacacat aacctcagtg
    78721 aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa tccagaactg
    78781 tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt ccgcgacgaa
    78841 tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg gtagccataa
    78901 tcaggccagg cgtacaaaac ccgcactgca ctgcgccgga tttcgcataa gcctgctgaa
    78961 catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct tttccttcag
    79021 cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac accgtacagg
    79081 caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt tcgcggagta
    79141 attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg ttaatggtgc
    79201 attcgatggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg cggcgacggc
    79261 ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact ctttactggc
    79321 ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag cttccagcgt
    79381 ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt
    79441 tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat ccagtcggca
    79501 atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg
    79561 cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacga ggatttcgtc
    79621 atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga cgaaacgaac
    79681 accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg tggcagaatc
    79741 tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac ggatctgcgg
    79801 tccagcaatg gacgtggccg cagcacataa cgccgggaga tgacgttgag ttataggatc
    79861 ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat cttccgccag
    79921 cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat aacggtcatt
    79981 gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg cctgcgggtt
    80041 gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag aagcaaaatc
    80101 aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat aaccgttttg
    80161 gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg ttacgaatag
    80221 cagcggcaac aggaattatt ggtggctcac ccagtgactt atgtccgtat gcggattgcg
    80281 gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg gtcggcattt
    80341 tgtaatccag cagattgggg ttacggacca cgccgctttt agcatcgatg atcatctctt
    80401 caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct tctgccagca
    80461 gtggattaag aatatgccct gaatcatgaa cgttgaggat gcggttgatg gtgactttgc
    80521 acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt gggttagtgg
    80581 tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg tagaaagcgt
    80641 ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc agcacgatat
    80701 ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg gcgtgagcga
    80761 tgattttctc ttttaataat agtgccgcac tgcgcagcgc aggcgcggca acatagctct
    80821 ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc ggtatcttga gttgaaataa
    80881 cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag aagacggtgt
    80941 cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca tcctgattca
    81001 tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag gtgtagctaa
    81061 aacaggcgac gccaacgccg cggcgcaaat tgccttgctg gttctggcat tctgcacggc
    81121 gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac cctgcgctgt
    81181 aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg cgtaaacgaa
    81241 tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa
    81301 atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg ttggtatagc
    81361 aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact ttattccccc
    81421 ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac agaacatcca
    81481 gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca atggtaaaag
    81541 cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact ttcaccggaa
    81601 tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca tcctgtttat
    81661 taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag ggaatatcca
    81721 gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa
    81781 ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga caatgttgaa
    81841 taacgggggt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt gtttgttgga
    81901 cattacccgt cgacatcgtg ctttgtttca gtaaattgcc accgttatgg attggtgcag
    81961 cgtcttctgc cagcgccgct tctggcgtgg tgataacggg taattcttgc cactcaatgc
    82021 tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg atggcaacgg
    82081 cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt
    82141 tttcgtcaag tgtccaggca tgcccagcag tagcaaatgg aatatcaggc acatcttccc
    82201 aggtaaaaat cgccagtacg cccggcaaac ttctggcttg ttcatcatta atacttacgg
    82261 cataaccatg tgcgataggg ctacgtacat atttcgcata acacatgccc gccataacat
    82321 aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg cgcatgcatg
    82381 attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt tgaacatttg
    82441 gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac gacgataagc
    82501 gttaggggag gtaggaagtg tgatgacgtt aaaaaaacac agatatgata cgaaacagga
    82561 ctctgaatag gggcagtgat acgtatcaat atgagtggcg ataaaatcaa tgtgataaat
    82621 tgggattatt acgcatcagg atgggaaata gggtaaaaag tcaggaagtt cgatacctct
    82681 acagcgaata ttttgctgat gatacttccc tttgccaaat gaaattaacc tctttctcta
    82741 ttaccacgtt tttccagaag caagagattg ggtatcaaga ggctggctgc tatgataagg
    82801 cgtcttgttt tttaagcgag gaaagatttt gagtgcggga cgcctgaata aaaaatctct
    82861 gggtatcgtg atgttgttat cggttggact gcttttggcg ggctgttcgg gtagcaaatc
    82921 atccgataca ggaacgtatt ccggctccgt ttacaccgtg aaacgggggg atacgctata
    82981 tcgtatttcg cgcaccacgg gaaccagcgt aaaagaactg gcgcgactga acggcatttc
    83041 ccccccttac accattgaag ttggtcagaa actaaaactg ggtggggcga aaagtagcag
    83101 tagtacacgt aaatcaaccg ccaaatcaac gaccaaaacc gcatcggtta caccgtcatc
    83161 agcggtaccg aaatcttcct ggccgccagt agggcaacgt tgttggttat ggccaacgac
    83221 agggaaagtt atcatgccgt attcgacagc agatggcggc aataaaggga ttgatatctc
    83281 agctccacgg ggtacaccta tttacgccgc gggtgcagga aaggtggtgt atgtgggcaa
    83341 ccagctgcgt ggctacggta atctcatcat gattaaacac agtgaagatt acattacggc
    83401 ttacgcccat aatgacacga tgctggtaaa taatgggcaa agcgtgaagg ctgggcaaaa
    83461 aatcgccact atggggagca cggatgcggc atctgttcgc ctgcatttcc agattcgtta
    83521 ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg cagggcagca agccaaaatg
    83581 ctgatggcga attaatcagc agtcagcagc gcggcacttg ctaaggagag cgtaaggttt
    83641 ataatgcctt acgcatctcg aagcgggcgt agttcaatgg tagaacaaga gcttcccaag
    83701 ctctatacga gggttcgatt cccttcgccc gctccagaat ctccccaggt ggagaagact
    83761 aaaaaagtct tctctgtcag caacttaaac agttacctat ttaatttgct taggagttct
    83821 accattgtga tccgccctta gtaactgtca cgttaagtta cacggtgaga tgacagtata
    83881 acctaacgtt ggcggcagtt gcacctctca caccgcgata gcacaacgtg aagataaagc
    83941 ccatcctggc attgtaatgt catcgacaga ctgaacacac gcatttaagc ccgatgtgcg
    84001 gccttgtggc tgcgtaaaca ggtttatggt gatattggtc cttgcaaacc atatgcaagc
    84061 tctatcagcg tttcagccac gctcccaacg ggtacacggt tatcctgata aggtgtgatc
    84121 tttctgacaa tctgatgaag tcgctacgct cccgcttgtt gctgtatcac caacggggct
    84181 ttatggtttc tattgtctat aataatctca caattcccct gcatgaggtt gatatgactg
    84241 ttagctacaa gattacttat gagcatagag acggtgatca aattagaatg gaagaggttt
    84301 tattccattc agagcatgaa ccaacaaaag atgaaattca tgatgcagta agaaaagata
    84361 ctgtgagatt taattctcca tctaatggag taacggctgt ttcagggttt aaagtgattt
    84421 cggttgtccc tgttccatga atggttatag aaggggctgg aagcccctaa ttattacttc
    84481 ttaatatcgc cagtgcattg atattcgata gtcacaagtg tttgcataca accagatgaa
    84541 gaaggttgtg aacattgaga agtgaatcca ccaaaaggct cggctcctga atatccccaa
    84601 gcggcacaac gcgctttggc tgcattcatg ccttgttgtg gatctacaac aggactttca
    84661 aacataccgt aagaataact catcctaact gtgccgtctg atttgcttcc accagttgga
    84721 acgagttgtt tttttgttgc acatccagaa ataatcaatg ttgaagcaag taacagcatt
    84781 aaacttttgc gcatgtaaat aatcccaaga gataatggtt atgtattgat ttaaagcgtt
    84841 tttagattaa tgcattaact tagcaaaatt aagtcttttc tgattgtttg cagagtaaaa
    84901 atgagttgct ttcgaaatta tttcggatgt ttattgtatt acggtttttc aaaccgtcta
    84961 actattgtcg ctattgaatg ggcaataata gcaagaggat tcatttcgtc attggaggca
    85021 acaaaaagtc ggtaactgat agctacaacg aatgaagcaa taacgagaaa gcggatagtg
    85081 ctaagatgtt ttgaagataa atgcataacg ccccctaaat gaatgtgagg gggattaaat
    85141 catgcaaatt tgttgtgtgc aatactggta atttatccta atagtctact attcacctca
    85201 atctacgtca accaatattt gatgtagatt tgattttctt ttaaaaacaa tatgttgcaa
    85261 tttgttgggt tttggggtta ggttgttttt tggacgtttc catcaagtgt aaagatacgt
    85321 ttagccttca tagtgagttc agttactgtc tttttctctg aaatcggcgc atacgtgatc
    85381 atcgcttaac gagaagatca gcgccgtcag gtccatgcag cgacaattct gaaatatcag
    85441 gaggcattac gcttcctgct ctttcttgat acgttgcacc gtttgtgtgc tgacactgaa
    85501 atcttttgct acgctccgaa ttgatccacc attcgcgaga gcggcagcaa ttcccgctcg
    85561 atcaattgct tttccattac cacgttttgc tatacgtcca gcggccttag ctgcggcgat
    85621 cccctcacgc tggcgctcaa gcatcatttc acgttccatt tgtgccactg ctgcaagcac
    85681 tgtgagcatc atattccctg cgggagagtt cttccctgtc ttaaagtctt ctttatggaa
    85741 atgcacagac acgccgcgct ctgttaattg ctcaaccgtt gccagtaggt cggctgtatt
    85801 acggcaaagt cgatcaatac tgtgaacatg caaaacatca ccttcccgca catagttcat
    85861 cattgcctca aactgtgggc gctgggtatt cttgcctgac gctttatctt caaagacttt
    85921 atcaagagct actccagcta attgacggtc tgtgttttgt tgcacagtcg ataccctaat
    85981 gtatccaata ttagccattt tctttattcc ttctgtgttg atttgtgtgt agtgattatt
    86041 gatcgtgttg attaggtgtg taaaggactt ttttcactac gaaattcgaa ggtttttagt
    86101 gtggtgattt agcctgcttt atcactacac gtcttgcatg agaatcatgt agttacattt
    86161 tggtaaaatt agcaagctat taaatagtgt gtttattttg aaaaaataat tttttgttgc
    86221 attaaaggaa acttgttcaa taaatgatag aacgtttatt gagcaacaat tttgcagtga
    86281 tgcgctatat gccgcattac ataaggcttt gctgatgata atgtaaaagt gaagaaagct
    86341 aacattgctc ttttaagcta cttttcattg aatttttagt atggaattac atagggataa
    86401 actatgaata ttctatgatt tacacgagat attcttgctg tttaattccg gctttggaga
    86461 tcaggcattc gcaagaatac gctaacgcgt tgcctgaaaa gccattgcac ataatagagg
    86521 tgagcgtata gcgaactatc aatgtgatca ttgcacgaat gataatgagt ggatatagat
    86581 tctatatatc gttagattgt agtttgatag ttaatgtgta atgtagattg tatgagcgta
    86641 tagcgaatgt tagttagatc aacaacaaac taacctttaa atctctttta tcttctcata
    86701 gatatggcta tgcgtagcaa ccgaaggtta gccaatcatc tctatgcagc cgtaaggctg
    86761 ttttgcatgt aatagttgtt tgttgttgtt agacagttac caatttatag atcagagata
    86821 gatgtgagcg ttgagcgata cactaatcat aacaacatct tcttactttt tagttagttg
    86881 ttagtaggtt attgttatgc gcgctattag cgcatgtata aaggtatcat ttttatctaa
    86941 agggctttag acatttttta cacctttttc tgtgcaagac acttaccagt gtgcacaata
    87001 cttatgcttt tctaaagccg ggaattttta agtttgattg atcaattgtt aactttaaaa
    87061 gttactttta acaatcactt agacgatcta aagccgcaat taacataata taagttgaca
    87121 tgcaagcact ttttaccgta tagtgcgggt ttcctaccta cccaggaaat gagttcct
//
LOCUS       DAZFKM010000004        86419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFKM010000004 DAZFKM010000000
VERSION     DAZFKM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1179
                     /gene="proV"
                     /locus_tag="ACHKAY_000286"
     CDS             <1..1179
                     /gene="proV"
                     /locus_tag="ACHKAY_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO5699027.1"
                     /translation="NLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIE
                     EGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAM
                     VFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGG
                     MRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLD
                     EAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTP
                     NGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAA
                     LIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG
                     "
     gene            1172..2236
                     /gene="proW"
                     /locus_tag="ACHKAY_000287"
     CDS             1172..2236
                     /gene="proW"
                     /locus_tag="ACHKAY_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO5699028.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2294..3286
                     /gene="proX"
                     /locus_tag="ACHKAY_000288"
     CDS             2294..3286
                     /gene="proX"
                     /locus_tag="ACHKAY_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO5699029.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3478..4662
                     /locus_tag="ACHKAY_000289"
     CDS             3478..4662
                     /locus_tag="ACHKAY_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5699030.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4786..5523
                     /gene="ygaZ"
                     /locus_tag="ACHKAY_000290"
     CDS             4786..5523
                     /gene="ygaZ"
                     /locus_tag="ACHKAY_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO5699031.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5513..5848
                     /gene="ygaH"
                     /locus_tag="ACHKAY_000291"
     CDS             5513..5848
                     /gene="ygaH"
                     /locus_tag="ACHKAY_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO5699032.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5939..6469
                     /gene="emrR"
                     /locus_tag="ACHKAY_000292"
     CDS             5939..6469
                     /gene="emrR"
                     /locus_tag="ACHKAY_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO5699033.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6596..7768
                     /gene="emrA"
                     /locus_tag="ACHKAY_000293"
     CDS             6596..7768
                     /gene="emrA"
                     /locus_tag="ACHKAY_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO5699034.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7785..9323
                     /gene="emrB"
                     /locus_tag="ACHKAY_000294"
     CDS             7785..9323
                     /gene="emrB"
                     /locus_tag="ACHKAY_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO5699035.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9387..9902)
                     /gene="luxS"
                     /locus_tag="ACHKAY_000295"
     CDS             complement(9387..9902)
                     /gene="luxS"
                     /locus_tag="ACHKAY_000295"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO5699036.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10052..11608)
                     /gene="gshA"
                     /locus_tag="ACHKAY_000296"
     CDS             complement(10052..11608)
                     /gene="gshA"
                     /locus_tag="ACHKAY_000296"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO5699037.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(11681..12109)
                     /gene="yqaA"
                     /locus_tag="ACHKAY_000297"
     CDS             complement(11681..12109)
                     /gene="yqaA"
                     /locus_tag="ACHKAY_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO5699038.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12106..12672)
                     /gene="yqaB"
                     /locus_tag="ACHKAY_000298"
     CDS             complement(12106..12672)
                     /gene="yqaB"
                     /locus_tag="ACHKAY_000298"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO5699039.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12955..13031)
                     /locus_tag="ACHKAY_000299"
     tRNA            complement(12955..13031)
                     /locus_tag="ACHKAY_000299"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12995..12997),aa:Arg,seq:acg)
     gene            complement(13095..13171)
                     /locus_tag="ACHKAY_000300"
     tRNA            complement(13095..13171)
                     /locus_tag="ACHKAY_000300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13135..13137),aa:Arg,seq:acg)
     gene            complement(13369..13445)
                     /locus_tag="ACHKAY_000301"
     tRNA            complement(13369..13445)
                     /locus_tag="ACHKAY_000301"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13409..13411),aa:Arg,seq:acg)
     gene            complement(13449..13541)
                     /locus_tag="ACHKAY_000302"
     tRNA            complement(13449..13541)
                     /locus_tag="ACHKAY_000302"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13505..13507),aa:Ser,seq:gct)
     gene            complement(13857..14042)
                     /gene="csrA"
                     /locus_tag="ACHKAY_000303"
     CDS             complement(13857..14042)
                     /gene="csrA"
                     /locus_tag="ACHKAY_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO5699040.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14277..16907)
                     /gene="alaS"
                     /locus_tag="ACHKAY_000304"
     CDS             complement(14277..16907)
                     /gene="alaS"
                     /locus_tag="ACHKAY_000304"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO5699041.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17035..17535)
                     /gene="recX"
                     /locus_tag="ACHKAY_000305"
     CDS             complement(17035..17535)
                     /gene="recX"
                     /locus_tag="ACHKAY_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO5699042.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17778..18839)
                     /gene="recA"
                     /locus_tag="ACHKAY_000306"
     CDS             complement(17778..18839)
                     /gene="recA"
                     /locus_tag="ACHKAY_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO5699043.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18919..19416)
                     /gene="pncC"
                     /locus_tag="ACHKAY_000307"
     CDS             complement(18919..19416)
                     /gene="pncC"
                     /locus_tag="ACHKAY_000307"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO5699044.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19561..20646)
                     /gene="mltB"
                     /locus_tag="ACHKAY_000308"
     CDS             complement(19561..20646)
                     /gene="mltB"
                     /locus_tag="ACHKAY_000308"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO5699045.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20902..21465
                     /gene="srlA"
                     /locus_tag="ACHKAY_000309"
                     /gene_synonym="gutA"
     CDS             20902..21465
                     /gene="srlA"
                     /locus_tag="ACHKAY_000309"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO5699046.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21462..22421
                     /gene="srlE"
                     /locus_tag="ACHKAY_000310"
                     /gene_synonym="gutE"
     CDS             21462..22421
                     /gene="srlE"
                     /locus_tag="ACHKAY_000310"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO5699047.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22432..22803
                     /gene="srlB"
                     /locus_tag="ACHKAY_000311"
                     /gene_synonym="gutB"
     CDS             22432..22803
                     /gene="srlB"
                     /locus_tag="ACHKAY_000311"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO5699048.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22807..23586
                     /gene="srlD"
                     /locus_tag="ACHKAY_000312"
     CDS             22807..23586
                     /gene="srlD"
                     /locus_tag="ACHKAY_000312"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO5699049.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            23691..24050
                     /gene="gutM"
                     /locus_tag="ACHKAY_000313"
     CDS             23691..24050
                     /gene="gutM"
                     /locus_tag="ACHKAY_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO5699050.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24117..24890
                     /gene="srlR"
                     /locus_tag="ACHKAY_000314"
                     /gene_synonym="gutR"
     CDS             24117..24890
                     /gene="srlR"
                     /locus_tag="ACHKAY_000314"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO5699051.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            24883..25848
                     /gene="gutQ"
                     /locus_tag="ACHKAY_000315"
     CDS             24883..25848
                     /gene="gutQ"
                     /locus_tag="ACHKAY_000315"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO5699052.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25845..27359)
                     /gene="norR"
                     /locus_tag="ACHKAY_000316"
     CDS             complement(25845..27359)
                     /gene="norR"
                     /locus_tag="ACHKAY_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO5699053.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27546..28985
                     /gene="norV"
                     /locus_tag="ACHKAY_000317"
     CDS             27546..28985
                     /gene="norV"
                     /locus_tag="ACHKAY_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO5699054.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            28982..30115
                     /gene="norW"
                     /locus_tag="ACHKAY_000318"
     CDS             28982..30115
                     /gene="norW"
                     /locus_tag="ACHKAY_000318"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO5699055.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30341..32593)
                     /gene="hypF"
                     /locus_tag="ACHKAY_000319"
     CDS             complement(30341..32593)
                     /gene="hypF"
                     /locus_tag="ACHKAY_000319"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO5699056.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32746..33273)
                     /gene="hydN"
                     /locus_tag="ACHKAY_000320"
     CDS             complement(32746..33273)
                     /gene="hydN"
                     /locus_tag="ACHKAY_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO5699057.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33422..34432)
                     /gene="ascG"
                     /locus_tag="ACHKAY_000321"
     CDS             complement(33422..34432)
                     /gene="ascG"
                     /locus_tag="ACHKAY_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO5699058.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            34692..36149
                     /gene="ascF"
                     /locus_tag="ACHKAY_000322"
     CDS             34692..36149
                     /gene="ascF"
                     /locus_tag="ACHKAY_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO5699059.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            36158..37582
                     /gene="ascB"
                     /locus_tag="ACHKAY_000323"
     CDS             36158..37582
                     /gene="ascB"
                     /locus_tag="ACHKAY_000323"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO5699060.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37696..38115)
                     /locus_tag="ACHKAY_000324"
     CDS             complement(37696..38115)
                     /locus_tag="ACHKAY_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5699061.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(38112..38423)
                     /locus_tag="ACHKAY_000325"
     CDS             complement(38112..38423)
                     /locus_tag="ACHKAY_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5699062.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(38597..39340)
                     /locus_tag="ACHKAY_000326"
     CDS             complement(38597..39340)
                     /locus_tag="ACHKAY_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699063.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(39383..39853)
                     /gene="hycI"
                     /locus_tag="ACHKAY_000327"
     CDS             complement(39383..39853)
                     /gene="hycI"
                     /locus_tag="ACHKAY_000327"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO5699064.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(39846..40256)
                     /gene="hycH"
                     /locus_tag="ACHKAY_000328"
     CDS             complement(39846..40256)
                     /gene="hycH"
                     /locus_tag="ACHKAY_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO5699065.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(40253..41020)
                     /gene="hycG"
                     /locus_tag="ACHKAY_000329"
     CDS             complement(40253..41020)
                     /gene="hycG"
                     /locus_tag="ACHKAY_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO5699066.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(41020..41562)
                     /gene="hycF"
                     /locus_tag="ACHKAY_000330"
     CDS             complement(41020..41562)
                     /gene="hycF"
                     /locus_tag="ACHKAY_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO5699067.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(41572..43281)
                     /gene="hycE"
                     /locus_tag="ACHKAY_000331"
     CDS             complement(41572..43281)
                     /gene="hycE"
                     /locus_tag="ACHKAY_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO5699068.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43299..44222)
                     /gene="hycD"
                     /locus_tag="ACHKAY_000332"
     CDS             complement(43299..44222)
                     /gene="hycD"
                     /locus_tag="ACHKAY_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO5699069.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44225..46051)
                     /gene="hycC"
                     /locus_tag="ACHKAY_000333"
     CDS             complement(44225..46051)
                     /gene="hycC"
                     /locus_tag="ACHKAY_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO5699070.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(46048..46659)
                     /gene="hycB"
                     /locus_tag="ACHKAY_000334"
     CDS             complement(46048..46659)
                     /gene="hycB"
                     /locus_tag="ACHKAY_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO5699071.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46784..47245)
                     /gene="hycA"
                     /locus_tag="ACHKAY_000335"
     CDS             complement(46784..47245)
                     /gene="hycA"
                     /locus_tag="ACHKAY_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO5699072.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47457..47807
                     /gene="hypA"
                     /locus_tag="ACHKAY_000336"
     CDS             47457..47807
                     /gene="hypA"
                     /locus_tag="ACHKAY_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5699073.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47811..48683
                     /gene="hypB"
                     /locus_tag="ACHKAY_000337"
     CDS             47811..48683
                     /gene="hypB"
                     /locus_tag="ACHKAY_000337"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO5699074.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48674..48946
                     /gene="hypC"
                     /locus_tag="ACHKAY_000338"
     CDS             48674..48946
                     /gene="hypC"
                     /locus_tag="ACHKAY_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO5699075.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48946..50067
                     /gene="hypD"
                     /locus_tag="ACHKAY_000339"
     CDS             48946..50067
                     /gene="hypD"
                     /locus_tag="ACHKAY_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO5699076.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            50064..51074
                     /gene="hypE"
                     /locus_tag="ACHKAY_000340"
     CDS             50064..51074
                     /gene="hypE"
                     /locus_tag="ACHKAY_000340"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO5699077.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51148..53216
                     /gene="flhA"
                     /locus_tag="ACHKAY_000341"
                     /pseudo
     CDS             51148..53216
                     /gene="flhA"
                     /locus_tag="ACHKAY_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(53253..53606)
                     /gene="ygbA"
                     /locus_tag="ACHKAY_000342"
     CDS             complement(53253..53606)
                     /gene="ygbA"
                     /locus_tag="ACHKAY_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO5699078.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(53683..53817)
                     /locus_tag="ACHKAY_000343"
     CDS             complement(53683..53817)
                     /locus_tag="ACHKAY_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699079.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            53893..56454
                     /gene="mutS"
                     /locus_tag="ACHKAY_000344"
     CDS             53893..56454
                     /gene="mutS"
                     /locus_tag="ACHKAY_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO5699080.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            56560..57216
                     /gene="pphB"
                     /locus_tag="ACHKAY_000345"
     CDS             56560..57216
                     /gene="pphB"
                     /locus_tag="ACHKAY_000345"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5699081.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(57267..58034)
                     /gene="ygbI"
                     /locus_tag="ACHKAY_000346"
     CDS             complement(57267..58034)
                     /gene="ygbI"
                     /locus_tag="ACHKAY_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO5699082.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            58230..59138
                     /gene="ltnD"
                     /locus_tag="ACHKAY_000347"
     CDS             58230..59138
                     /gene="ltnD"
                     /locus_tag="ACHKAY_000347"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO5699083.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            59135..60397
                     /gene="otnK"
                     /locus_tag="ACHKAY_000348"
     CDS             59135..60397
                     /gene="otnK"
                     /locus_tag="ACHKAY_000348"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO5699084.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            60394..61032
                     /gene="ygbL"
                     /locus_tag="ACHKAY_000349"
     CDS             60394..61032
                     /gene="ygbL"
                     /locus_tag="ACHKAY_000349"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO5699085.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            61037..61813
                     /gene="ygbM"
                     /locus_tag="ACHKAY_000350"
     CDS             61037..61813
                     /gene="ygbM"
                     /locus_tag="ACHKAY_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO5699086.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            61902..63266
                     /gene="ygbN"
                     /locus_tag="ACHKAY_000351"
     CDS             61902..63266
                     /gene="ygbN"
                     /locus_tag="ACHKAY_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO5699087.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(63305..63541)
                     /locus_tag="ACHKAY_000352"
     CDS             complement(63305..63541)
                     /locus_tag="ACHKAY_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO5699088.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(63552..64979)
                     /locus_tag="ACHKAY_000353"
     CDS             complement(63552..64979)
                     /locus_tag="ACHKAY_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO5699089.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(64979..65572)
                     /locus_tag="ACHKAY_000354"
     CDS             complement(64979..65572)
                     /locus_tag="ACHKAY_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO5699090.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            65719..66126
                     /locus_tag="ACHKAY_000355"
     CDS             65719..66126
                     /locus_tag="ACHKAY_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO5699091.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(66247..67239)
                     /gene="rpoS"
                     /locus_tag="ACHKAY_000356"
     CDS             complement(66247..67239)
                     /gene="rpoS"
                     /locus_tag="ACHKAY_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO5699092.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(67302..68396)
                     /gene="nlpD"
                     /locus_tag="ACHKAY_000357"
     CDS             complement(67302..68396)
                     /gene="nlpD"
                     /locus_tag="ACHKAY_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO5699093.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(68581..69207)
                     /gene="pcm"
                     /locus_tag="ACHKAY_000358"
     CDS             complement(68581..69207)
                     /gene="pcm"
                     /locus_tag="ACHKAY_000358"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO5699094.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(69201..69962)
                     /gene="surE"
                     /locus_tag="ACHKAY_000359"
     CDS             complement(69201..69962)
                     /gene="surE"
                     /locus_tag="ACHKAY_000359"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO5699095.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(69943..70992)
                     /gene="truD"
                     /locus_tag="ACHKAY_000360"
     CDS             complement(69943..70992)
                     /gene="truD"
                     /locus_tag="ACHKAY_000360"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO5699096.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(70989..71468)
                     /gene="ispF"
                     /locus_tag="ACHKAY_000361"
     CDS             complement(70989..71468)
                     /gene="ispF"
                     /locus_tag="ACHKAY_000361"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO5699097.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(71468..72178)
                     /gene="ispD"
                     /locus_tag="ACHKAY_000362"
     CDS             complement(71468..72178)
                     /gene="ispD"
                     /locus_tag="ACHKAY_000362"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO5699098.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(72197..72508)
                     /gene="ftsB"
                     /locus_tag="ACHKAY_000363"
     CDS             complement(72197..72508)
                     /gene="ftsB"
                     /locus_tag="ACHKAY_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO5699099.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(72702..73025)
                     /gene="ygbE"
                     /locus_tag="ACHKAY_000364"
     CDS             complement(72702..73025)
                     /gene="ygbE"
                     /locus_tag="ACHKAY_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO5699100.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(73075..73680)
                     /gene="cysC"
                     /locus_tag="ACHKAY_000365"
     CDS             complement(73075..73680)
                     /gene="cysC"
                     /locus_tag="ACHKAY_000365"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO5699101.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(73680..75107)
                     /gene="cysN"
                     /locus_tag="ACHKAY_000366"
     CDS             complement(73680..75107)
                     /gene="cysN"
                     /locus_tag="ACHKAY_000366"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO5699102.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(75109..76017)
                     /gene="cysD"
                     /locus_tag="ACHKAY_000367"
     CDS             complement(75109..76017)
                     /gene="cysD"
                     /locus_tag="ACHKAY_000367"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO5699103.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            76269..77306
                     /gene="iap"
                     /locus_tag="ACHKAY_000368"
     CDS             76269..77306
                     /gene="iap"
                     /locus_tag="ACHKAY_000368"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO5699104.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   77327..77782
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=77449..77477
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(77879..78172)
                     /gene="cas2e"
                     /locus_tag="ACHKAY_000369"
     CDS             complement(77879..78172)
                     /gene="cas2e"
                     /locus_tag="ACHKAY_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO5699105.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(78172..79092)
                     /gene="cas1e"
                     /locus_tag="ACHKAY_000370"
     CDS             complement(78172..79092)
                     /gene="cas1e"
                     /locus_tag="ACHKAY_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO5699106.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(79089..79739)
                     /gene="cas6e"
                     /locus_tag="ACHKAY_000371"
     CDS             complement(79089..79739)
                     /gene="cas6e"
                     /locus_tag="ACHKAY_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO5699107.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(79721..80467)
                     /gene="cas5e"
                     /locus_tag="ACHKAY_000372"
     CDS             complement(79721..80467)
                     /gene="cas5e"
                     /locus_tag="ACHKAY_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO5699108.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(80478..81533)
                     /gene="cas7e"
                     /locus_tag="ACHKAY_000373"
     CDS             complement(80478..81533)
                     /gene="cas7e"
                     /locus_tag="ACHKAY_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO5699109.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(81596..82084)
                     /gene="casB"
                     /locus_tag="ACHKAY_000374"
                     /gene_synonym="cse2"
     CDS             complement(81596..82084)
                     /gene="casB"
                     /locus_tag="ACHKAY_000374"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO5699110.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IR"
     gene            complement(82081..83643)
                     /gene="casA"
                     /locus_tag="ACHKAY_000375"
                     /gene_synonym="cse1"
     CDS             complement(82081..83643)
                     /gene="casA"
                     /locus_tag="ACHKAY_000375"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO5699111.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(83741..>86419)
                     /gene="cas3"
                     /locus_tag="ACHKAY_000376"
     CDS             complement(83741..>86419)
                     /gene="cas3"
                     /locus_tag="ACHKAY_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO5699112.1"
                     /translation="LLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYL
                     MVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEG
                     ARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPP
                     DRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVV
                     LADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFI
                     ENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLP
                     TMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDE
                     QTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEI
                     HACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLE
                     KDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWI
                     RNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRAS
                     QVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSP
                     PELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLL
                     IESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLST
                     RLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRL
                     WCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT        20289 a        23534 c        22174 g        20422 t
ORIGIN      
        1 aatctttata aaatatttgg cgagcatcca cagcgagcgt tcaaatatat cgaacaagga
       61 ctttcgaaag aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt
      121 ctggccattg aagaaggcga gatatttgtc atcatgggat tatccggctc gggtaaatcc
      181 acaatggtac gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt gctgattgat
      241 ggtgtggata ttgccaaaat atccgatgcc gaactccgtg aggtgcgcag aaaaaagatt
      301 gcgatggtct tccagtcctt tgccttaatg ccgcatatga ccgtgctgga caatactgcg
      361 ttcggtatgg aattggccgg aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca
      421 ctgcgtcagg tcgggctgga aaattatgcc cacagctacc cggatgaact ctctggcggg
      481 atgcgtcaac gtgtgggatt agcccgcgcg ttagcgatta atccggatat attattaatg
      541 gacgaagcct tctcggcgct cgatccatta attcgcaccg agatgcagga tgagctggta
      601 aaattacagg cgaaacatca gcgcaccatt gtctttattt cccacgatct cgatgaggct
      661 atgcgtattg gcgaccgaat tgccattatg caaaatggtg aagtggtaca ggtcggcaca
      721 ccggatgaaa ttctcaataa tccggcgaat gattatgtcc gtaccttctt ccgtggcgtt
      781 gatattagtc aggtattcag tgcgaaagat attgcccgcc ggacaccgaa tggcttaatt
      841 cgtaaaaccc ctggcttcgg cccacgttcg gcactgaaat tattgcagga tgaagatcgc
      901 gaatatggct acgttatcga acgcggtaat aagtttgtcg gcgcagtttc catcgattcg
      961 cttaaaaccg cgttaacgca gcagcaaggt cttgatgcgg cgctgattga tgcgccgtta
     1021 gcagtcgatg cacaaacgcc tcttagcgag ttgctctctc atgtcggaca ggcaccctgt
     1081 gcggtgcccg tggtcgacga ggaccaacag tatgtcggca tcatttcgaa aggaatgctg
     1141 ctgcgcgctt tagatcgtga gggggtaaat aatggctgat caaaataatc cgtgggatac
     1201 cacgccagcg gcggacagtg ccgtgcaatc cgcagacgcc tggggtacac cggcgactgc
     1261 accgactgac ggcggtggtg ctgactggct gaccagtacg cctgcgccaa acgtcgagca
     1321 ttttaatatt ctcgatccgt tccataaaac gctgatcccg ctcgacagtt gggtcactga
     1381 agggatcgac tgggttgtta cccatttccg tcccgtcttc cagggcgtgc gcgttccggt
     1441 tgattacatt ctcaacggtt tccagcaatt gctgctgggt atgcccgcac cggtggcgat
     1501 tatcgttttc gctctcatcg cctggcagat ttccggggtc ggaatgggcg tggcgacgct
     1561 ggtttcgctg attgccatcg gcgcaatcgg tgcctggtcg caggccatgg ttaccctggc
     1621 gctggtgtta accgccctgc tgttctgtat cgtcattggt ttgccgctgg ggatctggct
     1681 ggcgagaagt ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca tgcagaccac
     1741 gccagcgttt gtttatctgg tgccaatcgt catgctattt ggtatcggta acgtgccggg
     1801 cgtggtggtg acgatcatct ttgctctgcc gccgattatc cgtctgacca ttctggggat
     1861 taaccaggtt ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca gcccgcgcca
     1921 gatgctgttc aaagttcagt taccgctggc gatgccgacc attatggcgg gcgttaacca
     1981 gacgctgatg ctggcccttt ctatggtggt cattgcctcg atgattgccg tcggcgggct
     2041 gggtcagatg gtacttcgcg gtatcggtcg tctggatatg gggcttgcca ccgttggcgg
     2101 cgtcgggatt gtgatcctcg ccattatcct cgatcgcctg acgcaggccg ttgggcgcga
     2161 ctcacgcagt cgcggcaacc gtcgctggta caccactggc cctgttggtc tgctgacccg
     2221 cccattcatt aagtaactct gcacttgccc ggtgacgccg ggcattatca ccctgccaaa
     2281 aaaaggaata acaatgcgac atagcgtact ttttgcgaca gcgtttgcca cgcttatctc
     2341 tacacaaact tttgctgccg atctgccggg caaaggcatt actgttaatc cagttcagag
     2401 caccatcact gaagaaacct tccagacgct gctggtcagt cgtgcgctgg agaaattagg
     2461 ttataccgtc aacaaaccca gcgaagtgga ttacaacgtt ggctacacct cgcttgcttc
     2521 cggcgatgca accttcaccg ccgtgaactg gacgccactg catgacaaca tgtacgaagc
     2581 tgccggtggc gataagaaat tttatcgtga aggggtattt gttaacggcg cggcacaggg
     2641 ttacctgatc gataagaaaa ccgccgacca gtacaaaatc accaacatcg cacaactgaa
     2701 agatccgaag atcgccaaac tgttcgatac caacggcgac ggaaaagcgg atttaaccgg
     2761 ttgtaaccct ggctggggct gcgaaggtgc gatcaaccac cagcttgccg cgtatgaact
     2821 gacccatacc gtgacgcata atcaggggaa ctacgcggcg atgatggccg acaccatcag
     2881 tcgctacaaa gagggcaaac cggtgtttta ctacacctgg acgccgtact gggtgagtaa
     2941 tgagctgaag ccagggaaag atgtggtctg gttgcaggtg ccgttctccg cactgccggg
     3001 cgataaaaac gccgatacca aactgccgaa tggtgcgaat tatggcttcc cggtcagcac
     3061 catgcatatc gttgccaaca aagcctgggc cgagaaaaac ccggcagcag cgaaactgtt
     3121 tgccattatg cagttgccag tggcagatat taacgcccag aacgccatta tgcatgacgg
     3181 caaagcctca gaaggcgata ttcagggcca tgttgatggc tggatcaaag cccaccagca
     3241 gcagttcgat ggctgggtga atgaggcgct ggcagcgcag aagtaatttt tattcgggcg
     3301 gataaggcgt ttttgcctca tccgccgctc tgtacacaat gcctgatgcg acgctgacgc
     3361 gtcttatcat gcctacaaac cacatcgcac atttcaacaa gctatctttc atcccatatt
     3421 catcaacatc cgctattatt gatttccagc ttaatcatca cctgatgaac aaaaataatg
     3481 actaaaccta atcatgagct tagcccggcg ctgatcgtgc tgatgtctat cgccaccggt
     3541 ctggcggtcg ccagtaacta ttacgcccag ccgctactcg acaccatcgc gcgtaacttt
     3601 tccctttccg ccagttcggc aggctttatt gttaccgccg cgcaattggg ctatgcagca
     3661 ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac gccgccgcct gattgtctcg
     3721 atgaccttac tggcagcggg cggtatgttg attaccgcca gcagtcagtc gctggcgatg
     3781 atgctcctcg gtacggcatt aaccggttta ttctctgtcg tggcacaaat tctggttccg
     3841 ctggcagcga cgctggcttc accggataaa cgcggcaaag tggttggcac cattatgagc
     3901 ggtctgctgt tggggatatt gctggcacga accgttgccg ggttactggc aaacctcggc
     3961 ggctggcgca ccgtcttttg ggttgcctcg gtgttaatgg ctctgatggc gctagcatta
     4021 tggcgtggtc tgccacaaat gaaatcagaa acccacctca actacccaca gttgttgggt
     4081 tccgttttca gcatgtttat caacgataag atcctgcgca cccgcgcatt gcttggctgc
     4141 ctgacctttg ccaacttcag cattctctgg acctcaatgg cctttttgct tgccgctcca
     4201 ccttttaact acagcgatgg tgtcattggt ctgtttggac ttgcgggagc tgccggcgcg
     4261 ttgggcgctc gtccggcggg cggttttgcc gataagggta aatcgcacca caccacaact
     4321 ttcggtctgc tgctgctatt actttcatgg ctggcgatct ggtttggtca cacttccgta
     4381 ctggcgttga ttatcggaat cctggtgctg gatctcaccg tacagggcgt gcatatcact
     4441 aaccagacgg taatttatcg aatacaccct gatgcgcgta atcgcctgac cgcaggttac
     4501 atgaccagct actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa
     4561 catggcggtt gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg
     4621 gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt
     4681 cgtggcttta cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt
     4741 cattcagtct atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact
     4801 ccacagcctg ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt
     4861 gttattagtt atattccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc
     4921 tctcctctcg aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc
     4981 attaccgcga tgctggcagc cgggagtagt ttgtgggttg ctgcactgac cgtcatggca
     5041 atggatgttc gccatgtgtt gtatggcccg tcactgcgta gccgtattat tcagcgtctg
     5101 caaaaatcga aaaccgccct gtgggcattt ggcctgacgg atgaggtttt tgccgccgca
     5161 accgcaaaac tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc
     5221 ttcagttcat ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc
     5281 ttgctgcaag gttatcccgc cgttgaagct gcattaggtt ttatgcttcc ggcactcttt
     5341 atgagtttcc tgctcgcctc tttccagcgc aaacaatctc tttgcgttac cgcagcgtta
     5401 gttggtgccc ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt
     5461 gtctgtggct gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta
     5521 tgaggttctg ctgcttgggt tactggttgg cgtggcgaat tattgcttcc gctatttgcc
     5581 gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt ggcgcggtag gtattttgct
     5641 cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt
     5701 gatgcacgat acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag
     5761 tttctataaa acacgcagca ttatcatccc aacactgctt agtgcgctgg cctatgggct
     5821 cgcctggaaa gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat
     5881 ttactttatt tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat
     5941 ggatagttcg tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga
     6001 ttttccttat caggagatcc ttctgactcg tctttgcatg cacatgcaaa gcaagctgct
     6061 ggagaaccgc aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt
     6121 gattacgctg gagtctcagg aaaaccacag tattcagcct tctgaattaa gttgtgctct
     6181 tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat
     6241 cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg
     6301 tcacgagttt ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc aactctggtc
     6361 cgcgctcagc acaacagaaa aagatcagct cgagcaaatc acccgcaaat tgctctcccg
     6421 tctcgaccag atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc
     6481 gctcaaaaat ccagatttat aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc
     6541 tttttggcaa gcagctcggc tcagccgatg agttaagaag atcgtggaga acaatatgag
     6601 cgcaaatgcg gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg
     6661 tctgctcctc cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg
     6721 gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca tacgtggcag ggaatcagat
     6781 tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgattt
     6841 tgtaaaagaa ggcgatgttc tggtcactct cgacccgaca gatgctcgcc aggcgtttga
     6901 aaaagccaaa actgcactgg cttccagcgt tcgccaaacc caccagctga tgattaacag
     6961 caaacagttg caggcgaata ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga
     7021 ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca
     7081 cgcccgcgac gccgtcacca gtgcccaggc gcaactggac gtcgctattc aacaatacaa
     7141 tgccaatcag gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc
     7201 tgccaccgaa gtacgtaacg cctggctggc gctggagcgt acccgtattg tcagtccgat
     7261 gaccggttat gtctcccgcc gcgcggtaca gcctggggcg caaattagcc caacgacgcc
     7321 gctgatggcg gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca
     7381 gattgccaat atgcgtatcg gtcagccggt cactatcacc acggatattt acggcgatga
     7441 tgtgaaatac accggtaaag tggttggtct ggatatgggc acaggtagcg cattctcact
     7501 gcttccggcg caaaatgcga ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg
     7561 tatcgaactg gaccagaaac agctggagca atacccgctg cgcattggtt tgtccacgct
     7621 ggtgagcgtc aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttcaac
     7681 accggtcgcg gtaagtaccg cgcgtgaaat cagcctggca cctgtcaata aactgatcga
     7741 cgatatcgta aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac
     7801 cgctggaagg cgcgcaactg gtcattatga cgattgcgct gtcactggcg acattcatgc
     7861 aggtgctgga ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatctgggct
     7921 catcgctcag ccagggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga
     7981 tcccgcttac cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca
     8041 ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga
     8101 tcttcttccg cgtgattcag gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc
     8161 tattgctgaa taactacccg ccagccaaac gctcgatcgc gctggcgttg tggtcgatga
     8221 cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg ttatatcagc gataattacc
     8281 actggggctg gatattcttc atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac
     8341 tgcaaactct gcgcggacgt gaaacccgca ccgaacggcg gcggattgat gccgtggggc
     8401 tggcactgct ggttattggt atcggcagcc tgcagattat gcttgaccgc ggtaaagagc
     8461 tggattggtt ttcatcacag gaaattatca tccttaccgt ggtggcggtg gtggctatct
     8521 gcttcctgat tgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta
     8581 agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg
     8641 gcgctattgt tctgctgccg cagttgttgc aggaggtcta cggttacacg gcgacctggg
     8701 caggtttggc ctctgcgccg gtagggatta ttccggtgat cctgtcgccg attatcggcc
     8761 gcttcgcgca taaactggat atgcggcggc tggtaacctt cagctttatt atgtatgccg
     8821 tctgcttcta ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct
     8881 ggccgcagtt tatccagggg tttgcggtgg cctgcttctt tatgccgctg accaccatta
     8941 cgctgtctgg tttgccaccg gaacgactgg cggcggcatc gagcctctct aactttacgc
     9001 gaacgctggc ggggtctatc ggcacgtcga taaccacgac catgtggacc aaccgcgagt
     9061 cgatgcacca tgcgcagttg actgagtcgg taaacccgtt caacccgaat gcccaggcga
     9121 tgtacagtca actggaaggg cttgggatga cgcaacagca ggcatcaggc tggattgccc
     9181 agcagatcac caatcagggg ctgattattt ccgccaatga gatcttctgg atgtcagccg
     9241 gaatattcct cgtactgctg gggctggtgt ggtttgctaa accgccattt ggcgcaggtg
     9301 gcggcggagg cggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc
     9361 aattaattgt gaagatagtt tactgactag atgtgcagtt cctgcaactt ctctttcggc
     9421 agtgccagtt cttcgttgct gttgatgcgt acgtcacgtt ccagaatgct acgcgcaata
     9481 tcctgcgctt cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc
     9541 gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca
     9601 gcaacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc
     9661 attggcgaga tatcgataat ctctacaccc ttaccgttaa gatggttacg cataaaacca
     9721 gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg
     9781 cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc
     9841 acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc
     9901 atttagccac ctccggtaat tttttttaaa attttctgaa ctctttcttc ccaggcgagt
     9961 ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt
    10021 ggccactcac gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa
    10081 acggttcggt atcagcggtt tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg
    10141 ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtagg
    10201 cttctgcaaa cgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac
    10261 gggcagagaa agtcagatcg ggattatcga agcaggcaac cagttcatca cacactttct
    10321 gatacgcttc gccgccgtta atgctatcca gcgtttgcgc gacgcgtttc agatcgcgga
    10381 acagatcttt acccacctgc agtaacggga actgcgcggt ttcgcagccg atacccagcg
    10441 tcaggcccgg tttgcgacct tcaaggatca cccggttcca gttaacgcgc gtacaggcaa
    10501 gttcgctact gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga
    10561 ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgaac
    10621 gcacttcaat atattcaatg ccgccacgta acagcgcatc agaaggtgac tcgccgctgc
    10681 gggtaacgcg ttttggacga atcggcgcgt acagttcgtt ttcaatctgc agcacgttgc
    10741 tattgatttg cagcctctta ccgtctttct caataccaat cttcgcgtac tcttccgatg
    10801 gcgttttgat tgcctgttta aggcccgcta cgtactcgta aagatcgttg aaggtaatac
    10861 caagattgct ttgcgattta ttggtatagc cgagatcgct caaacgaaga gaggtcgcat
    10921 acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt ggttttcctt
    10981 gcaggaaaga agaacaaatc gccggagatg caccaaacag ataaggaatg acccaaccga
    11041 aacgatagta attgcggata acgcggaaat agcccgcaga aattttctct ttggcatcag
    11101 cgcccgagat atcaccgcac ttcgcttgcc agaatgccat tggcaaagag aaattgtagt
    11161 gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacgataca
    11221 gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt
    11281 ctgcgatgta gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg
    11341 tataacgatg cagatcgcgc ataaaggtca acatatgttc aatatcacca tccactggtg
    11401 taatgaattc cagcaatgct tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg
    11461 aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag
    11521 tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg
    11581 ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat
    11641 tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc
    11701 cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa
    11761 agattactgg cccccaggag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa
    11821 ccacgggcat ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag
    11881 ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag
    11941 ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacccag ggataactga
    12001 tcccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc
    12061 tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac
    12121 atccacggcg tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa
    12181 gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg
    12241 cgcgggttta tggtatttga cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa
    12301 tcccaggtgc gccagcaatg cctcagcgat ggcgctttca ctccccgttc ctacagccat
    12361 tgggcgacga ccatgccaac ttttcaccac ttcaacaaga ggaagcggct cgacactatc
    12421 cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc
    12481 ggcctgattc agctcaataa ttgcctgagc aatacgccag gtgggcgatc cattaagcgc
    12541 aatcattgcc tgaacatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt
    12601 acggtgcgta ggctccgtat ccaggattgt gccatccata tcaaaaatta aacctgcata
    12661 acgctcgtac atggtcttct cgcaagtcga aaaatatgac gctactttag cgtaattgct
    12721 tgattttgtc gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt
    12781 agaaatgaga tatttttgaa ggaaattttt gggagaagat ggtgcatccg ggaggattac
    12841 tcgccttgcg gctcgccctt cgggccgttg ctaaagcaac gttatcatcc ctggtgcctg
    12901 cggttaattc cgctacctct tcgatacctt cattgctgaa gatttcggag aatatggtgc
    12961 atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga
    13021 gctacggatg catcgggaaa atttatttta ctgctgatat tgataccgct actaaagcca
    13081 tatcaagtaa gagatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc
    13141 cgagtactct atccagctga gctacggatg catcgggaaa cttactttac tgcagatttt
    13201 ttgataccgc tactaaagcc gtatcaagta agagatggtg catccgggag gattactcgc
    13261 cttgcggctc gcccttcggg ccgttgctaa agcaacgtta tcctccctgg tgcctgcggt
    13321 tataccgcca cctcttccat accttcatcg ctgaaagtac gtagaatatg gtgcatccgg
    13381 gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg
    13441 gatgcaaatg gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc
    13501 ccttagcagg ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc
    13561 ttcgaagaac ttgtttatcg ctcatcgtcg ctgcgtggcg cacatattac tttctgagac
    13621 ttataagtca aacaattttt cctacacttt tatcgtttgc acacttcacg ttcaattagt
    13681 ctgtaaaaac gtcaaaaaga gtgttttatc aacagaaaaa tggaggtctg acagatagta
    13741 gtaatgcaaa aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag
    13801 agtgaaggag aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag
    13861 taactggact gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca
    13921 gaaacttcct tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact
    13981 gtcacggtga cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc
    14041 attctttgct ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat
    14101 catataacgc caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta
    14161 cattctgtta aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc
    14221 gatagcgtta atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat
    14281 tgcaatttcg cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca
    14341 tccgtaccac cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga
    14401 gcgaccatac caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct
    14461 gcaatcagag aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc
    14521 tgatttttta aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg
    14581 ctaaccaaca gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc
    14641 tgtgcggcag cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc
    14701 agtactgagc gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact
    14761 tcgcttaagc gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct
    14821 tcgatacgac gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata
    14881 tcaccagtgc ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc
    14941 agcacgcgta cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc
    15001 gccgcttcga gatccatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc
    15061 accaggtctt cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg
    15121 aagcgcagca ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc
    15181 tggcgcagcg cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat acgggcgcga
    15241 cgagcctcat caacatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt
    15301 ttaccgatgt gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc gaaggagaag
    15361 ttagcgcctt tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggt
    15421 gtttgatcca gcacgaccac agcttcctgg cctgcattga ttgcatcaac cgctttacca
    15481 tcaacaaaca gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca
    15541 gatgcactat caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc
    15601 gcgcgacgac gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg
    15661 cgctcacgac aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg
    15721 aaagccgttt caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac
    15781 gccagaccgc gctccagagt acgggcaaac tgctcttctt cagtcttcag cacctgctca
    15841 acctgcgcct gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga
    15901 ccaaccagtt tgtagaagaa ggtttctttc gcgccgagca tattgccgtg acgcactgcg
    15961 cgacgaatga tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc
    16021 gcgatcagga acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc
    16081 agatcggtcg cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg
    16141 tcatagttag agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta
    16201 tctacagacg gcttcggcag cggttccatc gtgccatctg cctggcggtt gaactgcatg
    16261 aagacgatgt tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggagga
    16321 ccaccccaaa tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca
    16381 gtgtcaccca tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg
    16441 cgaataatac gttcgcgcgg gatcccgact tctttttccc agatttcgta ggcttcgtcg
    16501 tcgctttcat agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg
    16561 gtcagcagtt cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag
    16621 ttgcccagca tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg
    16681 ttgtgtttac cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc
    16741 ttgtcgagcc caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa
    16801 gttgggtcgt tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg
    16861 aaaaagtcga gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa
    16921 tcaagctaac gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg
    16981 aaaagtggga ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc
    17041 ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa
    17101 acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt
    17161 cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt
    17221 cgcttcgcgg gaaatacctt tctgattcag ttcctgacga atacgtgcag gtccataacc
    17281 tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg
    17341 ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg
    17401 gccatttttg cccataatcg gtgccacgag tttacgtcgc agttcttgct cactgtgatc
    17461 gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga
    17521 cgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc gcagatgcga
    17581 cccaggtttt gctgataaac agatttaata gtcaatgagt gtgcagaatg ttcccgcagg
    17641 cccaattcgt tagaggatcg aggtgagagt aatttttcac gccagtgctg gcctgttccc
    17701 ctccccctaa ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca ttatctatta
    17761 tcatcaataa cgaacgatta aaaatcttcg ttagtttctg ctacgccttc gctgtcatct
    17821 acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg
    17881 atctctttcg cggtttccgg gttatctttc agccaggcag ttgcattcgc tttaccctga
    17941 ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgatcag cttctctttt
    18001 acgcccaggt caaccagttc gccgtagaag ttgatacctt cgccgtagag gatctgaaat
    18061 tcagcctgtt taaacggtgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta
    18121 cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag
    18181 gcgtagaatt tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca
    18241 attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc
    18301 gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg
    18361 atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg
    18421 tctactgcgc cagaacgcgc cagggcgtca cagatttcca gtgcctgctc gccggtgtcc
    18481 ggctgggagc acagcaggtt atcgatatcg acgcccagtt tacgtgcgta gattgggtcc
    18541 agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cacgctgcgc tgcggcgatc
    18601 acctgcagcg tcagcgtggt tttaccggaa gattccggtc cgtagatttc gacgatacgg
    18661 cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg
    18721 gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt
    18781 ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt
    18841 tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga agcaattata
    18901 ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc
    18961 atacgcagta gcctgacgac gcaccgcatc acggtcaccg ctgaagcatt cccgccgggt
    19021 aatgccttca ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc
    19081 actgccgcca tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc
    19141 tttcagtgca cctatcgcca tttccaccac gacgggttca ctcaccgcgc catgctgcgc
    19201 cagcgtctct tcgcgtacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa
    19261 tccgcgttca aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc
    19321 accggtacaa gactcggcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac
    19381 ctgttcactt aactgcatca gttcactgtc agtcatcaca acctctgtca gtcaaaaatt
    19441 tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag
    19501 gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc ttgcaaatag
    19561 ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg
    19621 ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc agtactggta
    19681 accggtaccg acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg
    19741 tggcgttaaa cctgccgtgg caagctgcga aatgctgtac ctggttttga agccatttgg
    19801 caagcctgga gcctgaccgt ttgccattac cgcgacctga tcgcctttca cccagccgtg
    19861 cgctttgaaa tagttcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg
    19921 cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtacgta
    19981 gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc
    20041 catcaacagg aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa
    20101 tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt
    20161 ttcaacgccg ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg
    20221 attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt
    20281 aataaatttt ttgcgataac ggagccatgc gccgttcgga cctgatggcg gtttcaccga
    20341 tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgct tcgcctggga
    20401 gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa
    20461 ctgctgggca ttcgggttat tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg
    20521 ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg
    20581 cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt
    20641 gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacgcc
    20701 ccacacaagg aagcggtagt cactgcccga tacggacttt acataactca actcattccc
    20761 ctcgctatcc ttttactcaa actttcaaat taaaatattt atctttcatt ttgagatcaa
    20821 aataacactt ttaaatcttt caatctgatt agattaggtt gccgtttggt aataaaacaa
    20881 taaatcctga aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc
    20941 gggctgttcc aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg
    21001 ttgattagcc tgctggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt
    21061 gaacgttttg ctcaacgttg cgccggtaac cctgtttccc gttacctact gttaccgtgc
    21121 attggcacgt ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa
    21181 aagtacaaac ccagctacta cgcggcggcc tcttatagct gccactcaat gaatggcctc
    21241 ttcccccata tcaaccctgg cgaactgttt gtttatcttg gcattgccag cggtctgaca
    21301 acgctgaacc tgccacttgg cccactggcg gtgagttatc tgctggttgg tctggtcacc
    21361 aatttcttcc gcggctgggt gaccgatctg accaccgcca tttttgagaa aaagatgggc
    21421 attcaacttg aacaaaaagt tcacctggca ggagcaacat catgacgcat attcggatcg
    21481 aaaaaggaac gggtggctgg ggcggcccgc ttgagctgaa agccacgccg ggaaaaaaaa
    21541 tcgtctatat caccgccggt acccggcctg cgattgttga caaactggca cagcttactg
    21601 gctggcaggc tattgacgga tttagagaag gtgaacccgc ggaggcggaa attggtgtcg
    21661 cggtaatcga ctgtggcggc acattacgct gcggcatcta tccgaaacga cgtattccca
    21721 ccattaatat ccactcgacg ggcaagtccg gtccgctggc gcagtacatt gtggaagata
    21781 tttatgtctc tggcgtaaaa gaagaaaaca tcactgtagt gggtgatgcg acaccacaac
    21841 cctcttccgt gggccgtgac tatgacacca gtaagaaaat caccgaacaa agcgatggtt
    21901 tactggcgaa ggtgggaatg ggcatggggt ccgccgttgc ggtgctgttt caatctggtc
    21961 gtgacaccat cgacactgta ttaaaaacca ttctgccgtt tatggcattc gtctcggcgc
    22021 tcattggcat cattatggct tctggccttg gtgactggat tgcccacggt cttgctccgc
    22081 tggcgagcca tccactgggt ctggtcatgc tggcgctcat ctgctccttc ccactgcttt
    22141 cacctttcct cggcccaggc gcagttatcg cacaggttat cggcgtattg attggcgtgc
    22201 agattggtct cggcaatatt ccgccgcatc tggctttacc ggcactgttt gccatcaacg
    22261 cgcaggcggc ctgcgacttc atcccggtcg gtttgtcgct ggcggaagcc cgtcaggaca
    22321 cggttcgcgt cggtgtccct tctgtactgg tgagccgctt tttaaccggc gcaccgactg
    22381 tactgatcgc ctggtttgtc tccggtttta tctatcaata gaggctgaaa catgaccgtt
    22441 atttatcaga ccaccatcac ccgtatcggc gcgagtgcca ctgacgccct cagcgaccag
    22501 atgctcatca cctttcgtga aggcgcgcca gcggacctcg aagagtattg cttcattcat
    22561 tgccacggcg agttgaaagg tgcactccat cccggtttgc aatttacgct cgggcagcat
    22621 cgctatccgg tgaccgctgt tggcagcgtg gcggaagaca accttcgcga actgggtcat
    22681 gtcaccctgc gcttcgatgg tttaaacgaa gcggaatttc cgggcactgt ccatgtggca
    22741 ggccctgtac ccgacgatat cgcgccggga tcggttttga agtttgaatc tgttaaggag
    22801 taaaaaatga atcaggttgc cgttgtcatc ggtggtgggc aaaccttagg cgcgttcctg
    22861 tgccacggtc tggctgccga ggggtatcgc gtcgcggttg tcgatattca gagcgacaaa
    22921 gccgcaaatg tggcacaaga aattaacgcc gaatatggtg aaagtatggc gtacggtttt
    22981 ggtgctgacg ccactagcga gcaaagcgtt ctggcgctct ctcgtggggt agatgaaatc
    23041 tttggtcgcg tggatttgct ggtctatagc gccggaatag caaaagcagc ctttatcagc
    23101 gacttccagc tcggcgattt tgaccgttcg ctacaagtga atctggtggg ttatttcctg
    23161 tgtgcccgtg aattttcgcg tttgatgatc cgcgacggga ttcagggtcg cattattcag
    23221 atcaactcga aatccggcaa agtgggcagc aaacacaact ctggctacag cgcagcgaaa
    23281 tttggtggcg tcgggctgac tcaatcactg gcgctggatc tggcggagta cggcattacg
    23341 gtgcattcac tgatgctcgg taacctgctg aaatcgccga tgttccagtc actgttgcca
    23401 caatacgcga ccaagctggg tatcaaaccg gatcaagtcg agcagtatta catcgacaaa
    23461 gtaccgctca aacgcggctg cgattatcaa gatgtgctga atatgctgct gttctacgcc
    23521 agtcctaagg cgtcgtactg caccggacag tcgatcaatg tcaccggcgg tcaggtgatg
    23581 ttctgatcaa cagcggagat ccattaagga tctccgtgag actatagaat gcctgatgcg
    23641 ctacgctcat caggcataca ggacttccgc cactacatta aggaaaagtt atggtatccg
    23701 cactcatcac cgtcgccgtt atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa
    23761 tttctcgttt taaccgtgcc ttcgacacac tatgccagca agggcgggtt ggcgtgggcc
    23821 gttccagcgg gcgctttaaa ccgcgggtcg tggtcgccat cgcgctggac gatcagcagc
    23881 gcatcgtcga caccttgttt atgaaaggac tgaccgtctt cgcccgaccg caaaaaattc
    23941 ccgcaattac cggtatgcat gcgggtgatt tacagcccga tgtgatcttt ccccatgatc
    24001 cactatcaca gaatgctcta tcattggcgc ttaaactgaa acgtggataa tttcgttgtg
    24061 aatgttactt gcttgcgaag ttatcatttt gaaacctaaa tcaggtaatc acgcccatga
    24121 aacctcgtca gcgtcaggcc gccattctgg agtatctgca aaagcagggt aaatgctcgg
    24181 ttgaagaatt ggcgcaatac tttgacacca caggcacaac cattcgcaaa gatctggtca
    24241 ttctggaaca tgccggaacc gtcattcgta cttatggcgg agtggtgttg aataaagagg
    24301 aatccgatcc gcctatcgat cataaaacac tcatcaacac ccacaagaaa gagctgattg
    24361 cagaagctgc cgttagtttt atccatgatg gcgattcgat cattcttgat gctggcagta
    24421 ccgttttgca gatggttccc ctgctctcgc gctttaataa catcacggtg atgaccaaca
    24481 gcctgcatat cgtcaatgcg ctatccgaac tggataacga acaaactatc ctgatgccag
    24541 gcggaacgtt tcgtaaaaaa tcggcctcat ttcacggaca gctggcagag aatgccttcg
    24601 aacatttcag tttcgataaa ttgtttatgg gcaccgacgg catcgatctc aatgcgggcg
    24661 taaccacctt caacgaggtt tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag
    24721 tgattttgat ggcggactca tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc
    24781 ttgaaagcgt cgataagctg attaccgacg caggtatcga tccggcgttt cgtcaggcgc
    24841 tggaagagaa agggatcgat gtgatcataa ccggagagag caatgagtga agcactactg
    24901 aacgcgggac gtcagacgtt aatgctggag ttgcaggaag caagccgttt accggaacgt
    24961 ctgggcgatg attttgttcg cgccgccaat atcatcctgc actgcgaagg caaagtggtg
    25021 gtttcgggaa ttggcaaatc gggccacatt ggtaagaaaa tcgccgcaac acttgccagt
    25081 accggcacac cggctttttt tgtccatccg gcagaagcac tgcacggcga tctggggatg
    25141 attgaaagcc gcgatgtgat gctgtttatc tcttactccg gtggcgcgaa ggaactggat
    25201 ctgattattc cgcgtctgga agataaatct atcgcgctgc tggcgatgac cggcaaaccg
    25261 acgtcaccgc tgggcctggc ggcaaaagcg gtgctggata tctccgtaga acgcgaagcc
    25321 tgcccgatgc accttgcgcc gacctccagc accgtcaata ccctgatgat gggtgacgcg
    25381 ctggcgatgg cggtcatgca ggcgcgcgga tttaatgaag aagattttgc ccgctcccac
    25441 ccagccgggg cactgggcgc tcgcttgctg aataaagtgc atcatctgat gcgccgtgac
    25501 gatgccatcc cacaggtggc gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc
    25561 cgcaccggtc tggggctggt ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt
    25621 accgacggcg atttacgtcg ctggctggtt ggcggcggcg cactcaccac gccagttaat
    25681 gaagcgatga cgacaggcgg caccacatta caggcacaaa gtcgcgccat cgacgccaaa
    25741 gagatcctga tgaagcgcaa aatcactgcc gcaccggtgg tggatgaaaa cggcaaactc
    25801 accggcgcaa ttaacctgca ggatttctat caggccggga ttatttaatc cttcaatccc
    25861 agacgtttcg ccagccgatg caggttggcg acgtcggttt ccagcatccg cgcgcaggca
    25921 gcccagttgt gatgattttg tgccagtgcc tggcgaatgg tttcacgctg gaacgcttct
    25981 gtcgcttcac gcaggttttg cttaacaacg ggcaccgccg ccgcttctgg cggcggcaac
    26041 gtcacctcag gaaaagcaaa atgttgtgcc tcaagaatca cttcatcgcc gctgcgagtg
    26101 gctctcgcca gcactaccgc ccgatgaata gcatgttcca gttcgcgcac gttccccgga
    26161 aaacgatagt gttgcagtaa atttcgcgct ccggcactta ataccacgcg ggagagcccc
    26221 agccgcaaac gacactgctc gcagaaatac cccgccagca gaatgacatc atcgccccgc
    26281 tcacgcagcg gcggcaccga aagtggaaac acgctcaggc gatgaaacaa atcggcgcgg
    26341 aatcgccctg ccagcacctc ttcgcgtaag tcgcggttag tcgccgccag cacgcgcaca
    26401 tcgacccgca aactacggtc atcgccaacg cgctgaatat cgccatactg caacaccctc
    26461 agcagcttgg cctgcaatgc caacgacaac tcgccgatct catcgagaaa cagagtgccg
    26521 ttatccgcca tttcaaactt cccgctgcgg ttactgatag cgccagtaaa cgctcctttc
    26581 acatgcccga acaactcact ttccgccaca ctttccggca gtgcggcaca gttgagatag
    26641 accagcggat tcaccgcccg tggcgaggct tcatgaatcg ctttcgccac cagctcctta
    26701 ccggttcccg tctcaccgct gatcagaacg ttgagatcgg acgccgccac aatctcaatc
    26761 tcttttttca attgcgtcat gcctggcgac aagccaatca tctgcgtctg tttcaccgct
    26821 tcaaacggcg cggcatcgcc cggcagcata ttctggcttt ccagttgttc aatcagcaac
    26881 gcattgctta acgctcccgc cgccagcgcg gcaatcagcc gtaactcttc gtcgctgaaa
    26941 acatcgaact gatcgggctg catcccgtcg agcgtcagtg cgccgatcag gttttgcccg
    27001 gcaaacaatg gcagaccaac gcaggcgtga accttcagac tctcctgccc aggaatcaaa
    27061 ccgtcatagg gatcgggcaa ttcgctgtct gcaggaaagc gcaccacgtc cccggcgcgg
    27121 gcaatcgctt caagccgtgg atgcccttcc agcgcaaagc gtctaccgag tacatccttt
    27181 gccagaccgt cgatggcaag cggaataaac tgccgcgaat cgtaacgtag caacgcagac
    27241 gcatcgcact ccagcacctg acgtagcgtg gtgatcaggc gctgaaaacg atcctgatga
    27301 ccaatcccac gctgcaattc gatggcgata ttcgccagca catcaacgga aaaactcatc
    27361 tttgcctcac tgtcaatttg actatagata ttgtcatatc gaccatttga ttgatagtca
    27421 ttttgactac tcattaatga gcataatttt atttatagag taaaaacaat cagataaaaa
    27481 actggcacgc aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg
    27541 ttgctatgtc tattgtggtg aaaaataaca ttcattgggt tggtcaacgt gactgggaag
    27601 tgcgtgattt tcacggcacg gaatataaaa cgctgcgcgg cagcagttac aacagctacc
    27661 tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat
    27721 ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc
    27781 atgcagaaga ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc
    27841 cgatctactg tactgccaac gctatcgact cgataaatgg tcatcaccat catccggagt
    27901 ggaattttaa tgtggtgaaa actggcgaca cgctggatat cggcaacggc aaacagctca
    27961 tttttgtcga aacaccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg
    28021 acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca
    28081 acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc
    28141 tgacgccgtt cagccgcctg gtaacgccga aaattaccga gatcctgggc tttaacttac
    28201 cagtcgatat gatagcgacc tcccacggcg tggtctggcg cgataacccg acgcaaattg
    28261 tcgagctgta cctgaagtgg gccgctgatt atcaggaaga cagaatcacc attttctacg
    28321 acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccaggga attgcggaaa
    28381 ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc
    28441 tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga
    28501 tgatgccgaa aatcgccggg ctggtggagg agatgactgg tttacgcttc cgtaacaaac
    28561 gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc
    28621 gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc
    28681 aggacgctct ggagttatgt cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg
    28741 cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga
    28801 cggctgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa
    28861 aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtt ccggataact
    28921 tcctctgccc ggaatgctcc ctcggcaaag acgtctttga agaactggca tcggaggcaa
    28981 aatgagtaac ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa
    29041 tattcgcaaa caggacgcca ctattccatt aaccctgatt gccgccgaca gcatggatga
    29101 gtacaacaaa cctgacctca gccatgttat cagtcagggg caacgtgccg acgaccttac
    29161 ccgccagacg gcgggtgaat ttgccgagca gtttaatctg cgcctgtttc cgcacacctg
    29221 ggtgacggat atcgatgccg aagcccgtgt ggtgaaaagc cagaataatc agtggcaata
    29281 cgacaagcta gtgctggcaa ccggtgccag cgcctttgtc ccgccagtgc ctgggcgtga
    29341 gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga
    29401 tgcccgacgc gtgttgattg tcggtggtgg cttgattggt agcgaactgg cgatggattt
    29461 ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt
    29521 aatgccaccg gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct
    29581 gttgttgaaa tctcagttgc aggggctgga aaaaacagat tctggcattc tggcaacgct
    29641 ggaccgccag cgcagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga
    29701 aaccgccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta
    29761 tctgcaaacc agtaatgccg atatttacgc gctgggcgat tgcgcggaaa ttaacggtca
    29821 ggtattgccg ttcctccagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct
    29881 cggcaataac acgccactga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt
    29941 accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaga ttaataccga
    30001 acgccaggga atggtggcgc gcggcgttga cgatgctgac cagcttcgcg cctttgtggt
    30061 cagtgaggat cggatgaaag aggcatttgg attgttgaaa acgttgccga tgtaggtggg
    30121 ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct tatccaacct acggggagcg
    30181 catgtgttgg tcacatactg cgttcatgtc gcatccacct actgtgccca aaatgtcgga
    30241 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcgt
    30301 ttacgccgca tccggcaatg gtgtccaaat gcaacacgtt ttatccgttc tggacttcac
    30361 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    30421 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    30481 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    30541 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    30601 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    30661 gttgattgtc caccagcggc attgtcaccg gatgcgtcac tccgtggcac gaagctgcga
    30721 gtgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    30781 gtgccgccgc cacggcatcg aacaaccgcc cacacgatga cgccagcggc gcgttaattc
    30841 cacgctcaat ggcccgcgcc agcacgctcc agttttgctg ttgcacactt gctgtttcag
    30901 aataattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    30961 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    31021 attcgcgata gttcacccgc agacactcgc cgccccacaa agcgccgttc tcccccatac
    31081 cgataccatc gagcgtcaaa gcaatgacat ccccgccatc cagcggccac tgatgctctg
    31141 ccagacacgc cgctgcgtgg gcatgatgat gcagcaccgt ttgcgtcggc atattcattt
    31201 cgctcgccca ctggctggag acatagcccg gatgcacgtc atgcacaacg tattgcggag
    31261 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    31321 cgtcacttaa atcgcccaga tgctgactca acaccgcttg ttcaccgcgc accaggcaga
    31381 aggtgttttt cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag
    31441 gcaaagccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    31501 gcaccaccga atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg
    31561 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    31621 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    31681 ggttcgcagg caacattacc ccgacttcgt taaggtcagg ggcgatatca tcacaaagct
    31741 caggaacgta ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactggc
    31801 gcgcagcgtc tggtaaaccg tcagccactg gcaacatgac tgccagcggt ttcgccgggc
    31861 ggtgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    31921 gaaatccgcc aatccctttg atggcgacaa ttttgcccat ttttaactgt gcgatagctg
    31981 cctgtaatgc cgcctcttgt tccgcatgtt caccatgact tacccattca agatgcgggc
    32041 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    32101 ctttatcaca ggctggacac agcggaaact ccgccattac ggtaaacggg cggtcgtaag
    32161 gcatggcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    32221 gataacgccg ttcgcctggg gtattcattt cggcaaggca agcagggcaa gtagcggcat
    32281 ccgggacaat ttgcgtattc atggcacctc ccgcgctctg gcggatagtg aactcggtgg
    32341 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    32401 agtgctgatg caattgaaca agaaacgttt ccgggtcttc cagcagccgg acttctacgc
    32461 catcgccgtc attacagaca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    32521 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg
    32581 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgtc cagccggaca
    32641 cgacagcgtc gcatccggca gtcacaggtc ggcgataccg cccgcttcgt attctacgaa
    32701 tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc
    32761 cgtacggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaattag
    32821 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    32881 gcatttattg gcttcagctt tgtcagcccg tacattcagc cccacgccgc tgttgcgaat
    32941 caccggacgt accaccactt ccatcgcgcc atacgggcaa gccaccacgc aggttttgca
    33001 accaatacaa cgttcctgca tcacatgaac aaacccttta tcacggctga tagcaccatt
    33061 cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat
    33121 gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca
    33181 gtcctgattt tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca
    33241 tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaatg
    33301 cgccgataca ttcacaaatc atgccagttt ttaatttact gttatttaag gaaattaatt
    33361 tctgtaatgc aggaaaaacg atgtcatcga cactagtgac gatgacatgt gatgacaatg
    33421 tttatcgcga aagggcaatg agtgagtcgc ggcagatcaa ttttccgctg aaggttttcg
    33481 gcggtgagaa atccccgcca tcgagcataa aaatcagccg tccaataatt tcctgaatca
    33541 tctcagtcac cggaattttt acgctggaga gcgccggaac gatatagggg gcaattgcga
    33601 tatcatcgaa tccgataact gacacctgct ctggcaccgc tacgccgcgc tcgtgcaacg
    33661 ctttcatcgc acctatcgcc atatcgtcgt tactggcaac taacgcgcta aatttagccc
    33721 cacgttcgag caacatttct accccttcgg ccccgctggc aggcgtccat ctaccgttag
    33781 cgataagttt ttcattgagc gcaataccgt gctgcgccag cgcgtcttta tatccggcaa
    33841 gacgttcaac gctggtggga gaatccatcg aaccggtaag gaaagcaatc tcctgatgtc
    33901 cggcgtttat caactctgcc acggcgttaa aactggtctg tttatgatcg caccagacgc
    33961 tatggctgct gtttttgcgc aggcggcgat taagcaccat tatcggctga ctgtgcgcgt
    34021 caatgatgtc atcgatctca tccacgctta aaaaacgcgg gtaaatcata atggcgtcgc
    34081 agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc ttcggcgctg tgtttaccat
    34141 ctgccaatag caactgccgc cctttctctt ccgccattcg cgcggcatga aacagtaatt
    34201 cactaaaata aatgccgtgg taaagcgtgt tggtcactac cagccccagc gtctgagtac
    34261 tcttcgccga caaattgcgc gccagcaagt ttgggcggta accgctctct tctaccgcct
    34321 gaaacacgcg atctttagtc tcctggctga cgtagccatt acctgaaagc acgcgggaaa
    34381 cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag catcgtcgtc atcattttca
    34441 tccctttaca cgcaatcaac gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc
    34501 ggcgggaaaa atattcaggt gaccggtttc acaaatataa aaaatgaaca attaactctc
    34561 ttgcttattt agtgacaact attcatgatt ttgtgaaacc ggtttcctaa ttccgtttca
    34621 gcatcggcat ttttccgtta cgtcgactga taacaactac atctacccta ctgataacag
    34681 gataaaatcc gatggccaaa aattatgcgg cgctggcacg ctcggtgata gcggcactgg
    34741 gcggcgttga taacatctcg gcggtcacgc actgtatgac gcgtttgcgc tttgttatca
    34801 aagatgacca gcttatcgac agcccgacgt taaaaaccat ccccggcgtg ctcggcgtgg
    34861 tacgtagtga caaccagtgt caggtgatta tcggcaatac cgtttcacaa gcctttcagg
    34921 aagtcgtcag cctactgccg ggagatatgc agcccgcact gcccgtgggt aaacccaaac
    34981 tcacgctgcg tcgcattggt gcagggatcc tcgatgcgct gatcggcacc atgtcaccgc
    35041 tgatcccggc gattatcggc ggatcgatgg tcaaactgct ggcgatgatc ctcgagatga
    35101 gcggcgtgct gacaaaagga tcgccgacct taaccattct gaatgtgatt ggtgacggtg
    35161 ctttcttctt cctgccgctg atggtcgcgg catctgccgc catcaaattt aaaaccaata
    35221 tgtcgctggc gattgccatc gcgggtgtgc tggtacatcc gagttttatt gaactgatgg
    35281 cgaaagcggc ccagggtgaa catgtcgaat ttgccctgat tccggtcacc gcggtgaaat
    35341 acacctacac agtgatcccg gcgctggtga tgacctggtg cctgtcatat atcgaacgct
    35401 gggtggatcg cattacaccg gcggtgacca aaaacttcct caagccaatg ctgattgtgt
    35461 tgattgccgc accgctggca atcctgctga ttggcccgat tggtatctgg atcggtagcg
    35521 ccatttcggc gctggtttac accattcatg gttatctggg ctggctttca gtcgccatta
    35581 tgggcgcgct gtggcctctg ctggtaatga ccgggatgca ccgcgtcttt acgccaacca
    35641 tcattcagac cattgccgaa accggcaaag aagggatggt catgccgtca gagatcggcg
    35701 ctaacctgtc gctgggcggt tcatcactgg cggtggcgtg gaaaacgaaa aacccggaac
    35761 tgcgccagac ggcgctggct gcggcggcat cagccattat ggcggggatt tccgaaccgg
    35821 cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat cgccagtctt atcagcggct
    35881 ttatttgcgg cgcggttgcc ggtatggcgg ggcttgccag ccactcaatg gcagcgccgg
    35941 ggctatttac cagcgtgcag ttcttcgatc cggcgaatcc aatgagcatc gtctgggtgt
    36001 tcgcggtcat ggcgctggcg gtcatgctgt cgtttatcct cacactgttg ctcggctttg
    36061 aggatattcc tgttgaggaa gcgactgccg aggcgcgaaa gcatcagagc gtacaaccga
    36121 ccgtcgccaa agaagtaagt cttaattgag gatgaaaatg tcagtatttc cagaaggttt
    36181 tttatggggc ggcgcgcttg ccgccaacca gtctgaaggt gcgttccgtg aaggtggcaa
    36241 agggctgacc actgtcgata tgatcccaca cggcgagcat cgaatggcgg tgaaactggg
    36301 gctggaaaaa cgttttcagt tgcgcgatga cgagttttat cccagccatg aggcgacgga
    36361 tttttatcat cgttataaag aagatatcgc cctgatggca gagatgggat tcaaggtttt
    36421 ccgtacctca attgcctgga gccgtctctt tccgcagggc gatgaaatca cgcccaatca
    36481 gcagggcatt gctttttatc gttctgtctt tgaagagtgt aaaaagtacg gtatcgaacc
    36541 gctggtcacg ttgtgccact tcgatgtgcc gatgcatctg gtcaccgaat atggctcctg
    36601 gcgtaaccgc aagctggtgg agtttttcag ccgctacgcc cggacctgct ttgaagcatt
    36661 tgatggtctg gtgaaatact ggcttacctt caatgaaatc aacattatgt tgcatagccc
    36721 gttctccggc gcgggtctgg tgtttgaaga aggtgaaaat caggatcagg tgaaatatca
    36781 ggccgcgcat caccagctgg ttgccagtgc gctagccacc aaaatcgccc atgaggttaa
    36841 cccgcaaaat caggtgggct gtatgctggc gggcggtaac ttctaccctt acagttgcaa
    36901 gccggaagat gtctgggcgg cgctggagaa agaccgggaa aacctgtttt ttatcgatgt
    36961 gcaggcgcgg ggcgcgtatc cggcttactc tgcccgcgta ttccgcgaaa aaggggtaac
    37021 catcgacaaa gcaccgggcg atgatgaaat cctgaaaaac accgtcgatt ttgtctcttt
    37081 cagctattac gcctcgcgct gcgcctcggc ggagatgaac gccaacaata gcagtgcggc
    37141 gaacgtagtg aaatcgctgc gtaacccgta tctgcaagtg agcgactggg gctggggtat
    37201 tgatccactc ggtctgcgta tcaccatgaa catgatgtac gaccgttatc agaagccgct
    37261 gtttctggtg gaaaacggcc tcggcgcaaa agatgaactt gctgccaatg gcgagattaa
    37321 cgatgactat cgcatcagct atttacgcga acatatccgc gcaatgggcg aagcgattgc
    37381 agatggcatt ccgctgatgg gctacaccac atggggctgt attgatttag tttccgcctc
    37441 tacgggtgaa atgagcaaac gctacggttt tgtctacgta gaccgtgacg acgcaggcaa
    37501 cggcacgctg acgcgcacac gtaagaaatc gttctggtgg tataaaaaag tgattgccag
    37561 taatggtgaa gatttagagt aagcaacagt gccggatgcg gcgtgaacgc cttatccggc
    37621 ctacatgtcg cacgaaccag gaaggcccga taagcgcagc gcatcgggca atctggggtt
    37681 tggcatcagt caaaatcaaa gaaaccattc cggcttaacg ccaaatcggg ctgaaagtgc
    37741 cttaatgtgg gatatcgtca gggatcgttg tccagacaaa atctgactga ccagggattt
    37801 agagccgatt tcgttcttca aatccgcata ggtcagatta tgttgatcaa tcagcgtgcg
    37861 aagtaacgcc acgccaacag gcattgccgc aacggctttg tcgaactctg caaacttttc
    37921 gttgttgttt tcatactctg caatgcggct cgccagaaag tcgataagtg gattggtgtc
    37981 atcttcttca atcaaatatt cgaccagttc cagtgcttca cgataatcat cttctgagtc
    38041 actgcctccc agaaagggga ctgcattgac cagttctctt gttgctttaa cagcgcgtgc
    38101 agcattagca gtcattcttt gttctctctg tagtaacgtg tcagtttgtc atactctgcg
    38161 tgattcgtta tgtgcttcac aaagaaccgc ttattaacaa agtttatgta ggcaataacc
    38221 ctaagattgt tcccgccgac atccagaacg taccatttgt tgcgatatct aaaattgtcg
    38281 aggttgggaa acgccgttcg catttcttct ggtgtagaaa aatccgtttc cttaataacc
    38341 cgatacaatg cctgatgagc cagagcgtcg ttaggatatt tacgcgcaga ctcttcaaat
    38401 ggcgctttag aaatgatgtg catttttaag ttttaacttc cttatagtaa gtaaacagcc
    38461 acatctgcga tgttctatcc ttatgtgttc ggaaactata gctcatcaaa tccaagttca
    38521 caatatgtaa acacttgtgc gcgatataat tacccccaaa atcctcatag ccactctgca
    38581 aaaagtcagt tctggttcac ccctcaagca catccccctt tatcaagcaa cgctgcgtct
    38641 gcgccagatc ttccgtttgc gggtcgcgcc cggcgatggg tttgaggacg agtttacggt
    38701 tgggggtata agtaaaacgc gtgagcgcgg gatagcttaa cttgccaaac cagccgtgaa
    38761 cacgctgcca tttggcgtct tttacctgat acagactggt tatccagtaa ccagaattaa
    38821 actgcatatc cagcaattgc gtgtgaccat tgccttgcaa atccagcaaa tcatctacgc
    38881 cagttttgct tgcggtgtag aatcccctgg gttcaaagac acagggacgg ccttcgcgcg
    38941 tgaacgtcat aagaattaaa tgtgcataag gagccagacc attaccggta cttggtaacc
    39001 agataaccag atcctgaatg ccgttacgat caagatcggc gcgatagagt tgccctttgc
    39061 ccgctatgtt tgccagcata cccacaggca cagtccagga tttaccggcg cggtctttag
    39121 cggttaattg ccagtaatct tcttcatttg gcatcttctc aagcgtcacg ccagcaacgc
    39181 ccactttcct aaaatgccag gtttgctcgg catcataagg catggtcagg agaggtttca
    39241 gcgcccgctt ttgcgcgtat tcgctgtcac tcattggcag atattcggta aagctggtgg
    39301 cctgtgcagg cagcactaag ctcaataaaa aagcgcacat ccctgttctg atgttcaatt
    39361 ccctgaattc cctgatggag aattattctt cttccaccgc taactgcgca aatccaccat
    39421 ttccttccca gccttccagt cgttgataaa cggtttctac cgcatcttta atcggctggg
    39481 tcatcgggta gtaaaagccg acgatatccg gctgaatgcc gaggaaaatc acttcaccaa
    39541 tgtcttcttt taactggtcg ataaggtaat tcaacggcat gttatgggta gtcatcataa
    39601 acatctcggc gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat
    39661 ccgtggcgtc gacaatcagc agtcgtgtcg ggcgcagttc acggatggcg acgatgtcgt
    39721 tttccggtgc actaccgccg tcaatcacca cccagttacc tttcggcgcg gcggcgcact
    39781 tttccgccag cagcgggcca gcgccatcat cgcccatcat gctattgcca acacagagta
    39841 aaacgtcagt cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca
    39901 tgcagcatac ttaacagcat tgtactccat tccttctgct gcgaagtttg cgctgcccgg
    39961 gctttatcga atgcgttggc cagcatcggc acatggttaa tgtcgatgac gatctcgcca
    40021 tatttcggta cgccttccat tttgcggcgg gcttcgctgc ccgcctccag agtggcaatc
    40081 catgccagat attcatccca tgggcaggtg agcgccgctt ccaggcaatc gataacccca
    40141 aggtggtgac caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca
    40201 tcgttctcat caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga
    40261 tacgcgcctc ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt
    40321 tttccgcttc cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtaaggtaat
    40381 catcggcaat ctgacgaccg taacgataac ccgccaggcg acgtgcttcg cgatccactt
    40441 tcacgcgcag cggctgcacc atatcgccat gcagaatctc agccggttgt tcatcctgct
    40501 cacccgggcc acgggcgtga attttctgct ccagcaggcc gagtgccatt gcaaagccgt
    40561 acagcgtggc ggcaggcgtt ggcgggcaac ccggaatgta gacatctacc gggacgattt
    40621 tatccgtacc gccccacacg cagtagagat cgtggaaaat accaccgctg ttaccgcagg
    40681 caccgtagga gatacaaatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg
    40741 atcgcattgc acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag
    40801 gaacgacttt aatgccgaag cgttctgcat caaacagcgg cgataaagtg gcgaaaattt
    40861 cgatttcgca gccgttgcag ccgccgcagt ccacgcggta aacataggca gaacgtttga
    40921 tttttttcag taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg
    40981 gaatgccgtt ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg
    41041 gcgagtcagt tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca
    41101 agtctcaaag ctttcgcggt ggttttccgc gcggctgtca ccattgtgct taagcagcgc
    41161 aatggcgtag tcgatctctt tctggacggc gaaaggacga ttgcagacgc gacagctgca
    41221 cagcacaaaa cgggactgtt gcaggaaatc ttctttcttc cacaccgcca gttcgtactc
    41281 ttgcgacagt ttgatcgctg ccgtcgggca gacttcttcg cagcgtccac agaagatgca
    41341 gcgcccgaga ttaaactccc acgccagctc accggtggcg aggtcagttt caaccgttaa
    41401 ggcgtttgac gggcaggcat tgacgcaggc cgcacagccg atgcactgct gcgggttctg
    41461 ctccggctta ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt
    41521 cgccgtgccg gttttgatga cttttttgat aaaggtaaac atggcgattc cttatttcag
    41581 cggcgagttt ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct
    41641 ctttttctta cgcacatcga ccacggtcat gcggtcggta caggagtagc aagggtccag
    41701 gctaccgata atcagcggcg catcggaaac ggtgttaccg cgcagcatgt agcgcagggt
    41761 cggccagttc gcgtaggtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc
    41821 ggtcatgctc cagtggatat cgtcgccgcg cggcgcttcg gcaaagccca gcgcgaaacg
    41881 gtgcggaatg taggtaaagc cttccaccat cagcggaccg cccggcaggt tatccagacc
    41941 gtagtcgatc atgttcagcg cggtataaac ttcgttgata cgcactttca gacgggaaat
    42001 aacgtcgcag ccctgctcgc tgtggacttc cattggcagc aggccataac cgacaaacgg
    42061 gtgatcggcg cgagtatcgc gggcgtgacc gctggcgcga accatcgggc caacgttact
    42121 gaaatcgcgg gcgatttccg ggtccagacg accaatgccg acggtgcgct gttccatgtt
    42181 cggcgtactc agcagcacat ccaccagctc ctgcacttca cgacgcatct gttgcgccag
    42241 ctggcgggtc tggatcatgt catctttcaa cagatcgcga cgaatcccgc cgatcaggtt
    42301 caggccgtag gttttacgcg caccggtgag gatctctgcc attttcatgg aggtttcacg
    42361 cacgcggaag aactgcataa agccggagtc aaagccggta aagtggcagg cgagaccgag
    42421 gttgagcaga tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc
    42481 acgttctggc acctgaatac ccatcgcgtt ttctaccgac gtggtgtagg cggtgctgtg
    42541 ggcaaagccg cagatcccgc acacacggtc agagaggaag gtcacttcgt tgtaacccat
    42601 acgggtttcc gccagttttt ccatgccgcg atgaacgtag aacagacggt agtcggcgtc
    42661 gataatgttt tcgccatcga cgaacagacg gaagtggccc ggttcgtcag aagtgacgtg
    42721 cagcggacca atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta
    42781 ggtttcagca tcggtagtcg gtgccggacg ctgacgataa tccatgctgt ctttacgcag
    42841 cggataaagt tcatccggcc agtcatccgg cagcaccaga cgacgttcat ccggcagacc
    42901 aaccggaatc aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac
    42961 gcgcggcgtc acggacggat actccggttt gttggcgtca acttcgacgc gaaccgttac
    43021 ccaacacttg gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag
    43081 tttgcgttcg tcgttaccaa acagcaccga cagccagcca ccctgcttgt agtagagaaa
    43141 ctccaccact tccggcaggt agttcacctt cacggtcacg gtcagctgat ctttggtctg
    43201 ccaggcgtgg tccagcacga cgcccggaaa tgcctcattc agcgcggcga gataatgttg
    43261 acctaatttt tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg
    43321 aacgctaaaa atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc
    43381 agacgggcca tgctgttttc gaacagcgcg ataaccagga cgccgacgac cagtttgacg
    43441 atggcaatca ccagcgccag cagcagccca ccggcggtga aggtttccat ctgtccccac
    43501 gggataaata cgccgacgaa catctgcagc accaccagct gtttcaggct gataccccat
    43561 ttcatgacgc caaagccgct gccgctgtat tcagagagcg ggccttcctg cagctcctgc
    43621 tcggcttccg ccaggtcgaa cggcagtttg cccatttcga taaaggtggc gaacgcacag
    43681 gcacaaagcg ccagtaccag cgggatgctc tggctcagcg gccagtgata aacggtgtcg
    43741 gtgatgttgc tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc
    43801 agcatcggtt cgaccagcac gccaagcatc gcttcacggc tcgcgccgat agcggtaaac
    43861 gggctaccgg tatccagacc agaaatggca aagaagaaac gcgcgatggc aaagagatac
    43921 agtaaggtga ttaaatcacc cagttgcggc agcggagaac cgacggtcac caccggcagc
    43981 gcagtagcga tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag
    44041 ccggaggcat ccgggccaac gctctggcgg cccagcagtt tgataatgtc acgatactcc
    44101 tgcaacacac ccggcccacg acggttatgt aagcgggctc gcgccacgcg ggtaataccg
    44161 gagagcagcg gcgcaacggc aaatagcacc agcgcctgaa ttaacggata taaaacactc
    44221 attctcaggc tcctcgtgaa acaataatca ccaccagtac cgccagttca accagcgcca
    44281 tccggcggaa cagcaacgca ctcccctcgc actgccagcc cggcaccaga gacaccggat
    44341 tcagccattt gcgtagtttc agcaccggcg caaacgcctg tttcaccggc atggcaaaac
    44401 cgtgagcggt aatcaccatt gatttttcgt ggtcgtagcc gcacacccag gccgcgccgc
    44461 gggaacgcga tggcaaacga tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg
    44521 gacaggcaat cagcagcaat gtgatcattg gttgagaaac ggtggtgtta gcaggctcca
    44581 gcggcagagg tacagcagca gacagcatcg gcagcagcca cggcgcagca acaccgccaa
    44641 ttacgcagca aatcgccagt gccactacgc ttaccctcat caggagcggc gcacaggtgg
    44701 cgttttcggc ttctttggta cgcggcgcgc cgaggaacgt gacgccatag actttcgcca
    44761 tacacatcac cgccagcgca ccggtaattg ccagacccac agcgagcagc ggccccagca
    44821 gacgggcaac aaacgcgccg ctattgctca gtttgaagaa ggattgatag ataacccatt
    44881 ccccggcaaa cccgttcagc ggcggcagcg cagccattgc cattaacccg actaacatgg
    44941 cgatggagat aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac
    45001 cggtacggaa ccagacgctc cccgccccga ggaacagtac gcttttgaac aggctatggt
    45061 taagcagatg gtacagacca ccgaccaggc caagagcaat cagcgccggt tgttcgagcg
    45121 cgatacccgt tacgccagcg cccagcccca gcaggataat gccgatattt tccagggtgt
    45181 ggtaagccag caggcgctgg atgttgtgct ccatcagcgc atacagacca ccgacaaacg
    45241 cggtgatcat gccgagcacc agcagcgcga tcccccacca cagcggtgca ttaccgccca
    45301 gcagtgacag ggttaaaatg cccagcaggc caattttcat gactaccgta gaaaacaacg
    45361 cggcagctgg cgcagaggcg ttagcatgtg cctgcggcac ccagccgtgc agcggaataa
    45421 tcccggcgag caggccaaag ccaatcacac cgagcagcca gatatcggaa ccgagcggca
    45481 gctgttgcat acgcatatcc agcaggcgca gatccagcgt gccataacgc tgccacagca
    45541 gccagcaagc aatcgccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc
    45601 cctctttgct gttgctagtg aggaacaccg cgcacagggc catgatttcg gccattacca
    45661 cgaacatgcc gaggttgctg gcaatgacgg cgcagacggc ggcagccatc aacatattga
    45721 tctgcaagcc gttgcacttc acctgcgcgt ggcgatgcca gtcaatgtta tagaggctga
    45781 caaacagacc gcaaaggccg agggtaatca gccaaatcgc gttaagcggg gagatttgca
    45841 catcgtagct taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agcacagtga
    45901 agcccgcggc tgccgtatac aggctaccaa ccgcgccgcc aattccggct atccagccac
    45961 ttagcgtctt ttgaaaagaa aagagaaatg ccagaacagc ggcggcgaca aaccacgcca
    46021 cgccgctatt gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa
    46081 caaggtgaga tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg
    46141 ggcgatatcg gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacgcaggc
    46201 cggaccttgt tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg
    46261 cacccagtca agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg
    46321 ggtattggcg tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa
    46381 cgggcaggcg atgccgcaca gcttgcagct tacgcacagg ctttcattca actgcacggc
    46441 cccatcaacg cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca
    46501 gtggtgacag agctgcggcg cagattcttt ttcattcagc atcactctca ggcgcggcat
    46561 tgattgcagg ccgtgctggc gatgcgtctc tgaacaggcg gcctcacaag tgtggcagcc
    46621 gatacagagc gtggagtcag caattacaaa acgattcacc aggcattcct caggtgattg
    46681 tcatttttga cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat
    46741 ccccaaacag gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt
    46801 gtccctttac cagttggctt aaattcaccg gcacattgtt ttcaatagcg gtgtataagc
    46861 tgtcgtaaac gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga
    46921 tgttgacctt gccatcaatc atgccgtcaa tgctcagttg cgcctgcgca atccgctgtt
    46981 tgtcttcgcc atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg
    47041 ccagggtgac gtaaatgcga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg
    47101 cgcagctcat ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat
    47161 tgcagtagat gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt
    47221 tctcgcttat ttcccaaata gtcattgtca ggttacccgt ttaacagaga tgccagcttt
    47281 aagcattttc tgtgccaact tttaatttat tgttattaaa gagattttta agctaaagat
    47341 gaatttcgtc gccgtgtcga cgtgtcattt cgacatcatc gacattattc accgcaggga
    47401 taatcaacac tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc
    47461 acgaaataac cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg
    47521 gcgcaaaacg cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca
    47581 gctctcttgc cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac
    47641 tgcacctcga agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc
    47701 tactgaccca gcgcgtccgc cgctgtccac agtgtcatgg tgacatgctg cagattgtgg
    47761 cagacgacgg tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa
    47821 catgcggttg cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg
    47881 cgtttcgtag cgcgccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta
    47941 aagcgcctga atttaccccc agccagactg aagaaggcga cctgcattac ggtcatggcg
    48001 aagcgggcac tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg
    48061 tgctggacaa aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc
    48121 aactggtgct caacctggtt tccagcccag gttccggtaa aaccaccctg ctgacggaaa
    48181 ccttaatgcg cctgaaagac agcgttccgt gcgcggttat tgaaggtgac cagcaaaccg
    48241 tgaacgatgc cgcacgcatt cgcgctaccg gcacaccagc gattcaggtg aacaccggta
    48301 aaggctgcca tcttgacgca cagatgattg ccgacgccgc accgcgtctg ccactggacg
    48361 ataacggtat tctgtttatc gaaaacgttg gcaacctcgt atgcccggcc agcttcgatc
    48421 tcggtgaaaa acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga
    48481 aatatccgca tatgtttgcc gccgcctcgc tgatgctgct caacaaagtt gacctgttgc
    48541 cgtatctcaa ctttgacgtt gagaagtgcc tcgcctgcgc ccgcgaagtc aatccagaaa
    48601 ttgaaatcat ccttatttcc gccaccagcg gtgaagggat ggaccagtgg ctgaactggc
    48661 tggagacaca gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca
    48721 ggcaaaagtc gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg
    48781 cgatgaaaac ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg gctttgccat
    48841 gagcgtaatt aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga
    48901 cgttgagccg gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat
    48961 gaatatcgcg cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca
    49021 catctctctt acaccgccga acgccctctg cggattatgg aagtgtgcgg tggtcatacc
    49081 cacgctattt ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac
    49141 ggtccgggtt gcccggtgtg cgtactgccg atgggtagaa tcgacacctg cgtggagatt
    49201 gccagccatc cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt accagggaaa
    49261 cagggatcgc tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg
    49321 atggatgcgt tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta
    49381 ggttttgaaa ccaccatgcc gaccaccgcc atcactctgc aacaggcgaa agcgcgtgat
    49441 gtgcagaatt tttacttctt ctgccagcac attacgctta tcccgacgct gcgcagtttg
    49501 ctggaacagc cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt
    49561 atcggcactg atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtcgct
    49621 ggtttcgaac cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg
    49681 gcccacagca aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg
    49741 gcgcaacagg cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta
    49801 ggcgtgattg aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa
    49861 gcacatttcc gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggtgag
    49921 gtattaacgg gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct
    49981 caaaccgcat ttggtgcact gatggtctcc tccgaaggag cgtgcgccgc gtggtatcag
    50041 tatcgtcagc aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc
    50101 aggcgatgca gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg
    50161 cagagcagga agatcaggca cgtcttgatc tggcgcagct ggtagcagaa ggcgatcgtc
    50221 tggcgttctc caccgacagt tacgtcattg acccgctgtt ctttcctggc ggtaatatcg
    50281 gcaagctggc gatttgcggc actgcgaatg acgttgcggt cagtggcgct attccgcgct
    50341 atctctcctg tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag
    50401 tgaccagcat ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta
    50461 aagtggtgca gcgcggcgcg gcagataaac tgtttatcaa caccgctggc atgggcgcaa
    50521 ttccggcgaa tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttactggtta
    50581 gcggcacgct cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg
    50641 atggcgaact ggtcagcgac tgcgcggtac tgacgccgct tattcagacg ctgcgtgaca
    50701 ttcccggcgt gaaagcgctg cgtgatgcca cccgtggtgg tgtgaacgcg gtagttcatg
    50761 agttcgcggc agcctgcggt tgtggtattg aaatttcaga agcggcactg ccggttaaac
    50821 ctgccgtgcg cggcgtttgc gaactgctgg gactggacgc ccttaacttt gccaacgaag
    50881 gcaaactggt gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt
    50941 cccatccact gggaaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc
    51001 gtcttgccgg tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc
    51061 cgcgtatatg ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc
    51121 tttgcggtgc tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag
    51181 caagggttgt tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt
    51241 gaggctcttt cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg
    51301 tggcaagcgc agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga cacccccatt
    51361 aaatatgaag acgaaactgt tctggcacac ggtccggtac gccgcatttt gtcgcgccct
    51421 gatacgctgc attgcagtta cgaagaattt tgtgaaacct ggccgcagct gggctatacc
    51481 ccaaatttgg tcactattgc ctgatgccac tggcggcgga agggcatatt tttggtggct
    51541 gtgaatttat tcgttatgac gatcgtccct ggagcgaaaa agagttcaat cgtctgcaaa
    51601 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gtcaacaatg
    51661 tcgactatga gttgttatgc cgggagcgcg ataacttccg catcctggtt gccattacca
    51721 acgcggtgct ttcccgcctg gatatggacg aactggtcag cgaagtcgcc aaagaaatcc
    51781 attactattt cgacattgac gatatcagca ttgtcttacg cagccaccgt aaaaacaaac
    51841 tcaacatcta ctccactcac tatcttgata aacaacatcc cgcccacgaa cagagcgaag
    51901 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatca
    51961 atctccacga gcgggacgat ttagccccct atgaacgcat gttgttcgac acctggggca
    52021 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    52081 tgaaactggc gcaatgcgaa gagaaagtgt ttaccactac caatctgaat ttgctgcgcc
    52141 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    52201 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    52261 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    52321 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggcacgggta
    52381 aagagctgat tgcccgcgcg atccataatc tcagtgggcg taataatcgc cgcatggtca
    52441 aaatgaactg cgcggcgatg cctgccggat tgctggaaag cgatctgttt ggtcatgagc
    52501 gtggggcttt taccggtgcc agcgcccagc gcattggtcg ttttgaactg gcggataaaa
    52561 gctctctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    52621 gtgtattgca ggaacaggag tttgaacgtc tcggcagcaa caaaatcatt cagacggacg
    52681 tacgtttaat tgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    52741 gtagcgatct ctattaccgc ctgaacgtat tcccgattca cctgccgcca ctacgcgagc
    52801 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    52861 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaac atggagtggc
    52921 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc ggtattgcta acacgcggta
    52981 acgtgctaca gctgtcattg ccagatattg ttttaccgga acctgaaacg ccgcctgccg
    53041 caacggttgt cgcccaggag ggcgaagatg aatatcagtt gattgtgcgc gtgctgaaag
    53101 aaaccaacgg cgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    53161 cgaccctgct gtcacggatg aagcggctgg gaattgataa atcggcattg atttaactgc
    53221 aaattgccgg acagatctgc ctgtccggca tactattcac gaggtttttt cggacgatat
    53281 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcac ggttaagatc
    53341 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    53401 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    53461 acacatttat ccagccgctt ttgcgcgtaa acaaacaacg cctcgtaatg ctccggctcc
    53521 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    53581 tcacgagaga tacgcttgcc ggacatgctg cctccatact cttaaggtgt atttatatta
    53641 caacttaatt tgaaagggga ctatggctct gaaggaggag gtttattcaa ccgacgcaat
    53701 tttgcgtact tgcttcataa gcatcacgca aaagctgcaa aacagcatct ttcccggaac
    53761 cagcatcaag aactcgcagt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg
    53821 cgccttatgt gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc
    53881 ggacataacc ccatgagtgc aatagaaaat ttcgacgccc atacgcccat gatgcagcag
    53941 tatctcaggc tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt
    54001 tatgaactgt tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc
    54061 aaacgcggtg cttcggcggg agagccgatc ccgatggcgg ggattcccta ccatgcggtg
    54121 gaaaactatc tcgccaaact ggtgaatcag ggagagtccg ttgccatctg cgaacaaatt
    54181 ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca
    54241 ggcaccatca gcgatgaagc cctgttacag gagcgtcagg acaacctgct ggcggctatc
    54301 tggcaggaca gcaaaggttt cggctacgcg acgctggata tcagctccgg gcgttttcgc
    54361 ctgagcgaac cggccgaccg cgaaacgatg gcggcagagc tgcaacgcac taatccggcg
    54421 gaactgctgt atgcagaaga tttcgccgag atgtcgctga ttgaaggccg tcgcggcctg
    54481 cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa
    54541 tttggcaccc gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg actttgtgct
    54601 gccggttgtc tgttgcagta tgcgaaagat acccaacgca cgaccctgcc gcatattcgt
    54661 tctatcacca tggaacgtga gcaggacagc atcattatgg atgccgcgac acgtcgtaac
    54721 ctggaaatta ctcagaacct ggcgggcggt gcggaaaata cgctggcttc tgtgctcgac
    54781 tgcaccgtca cgccgatggg tagtcgtatg ctgaaacgct ggctgcatat gccagtgcgc
    54841 gatacccgcg tgttgcttga gcgccagcaa actattggcg cattgcagga tttcaccgcc
    54901 gagttgcagc cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct
    54961 ttacgaactg ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg
    55021 gagctgcgtg cgcagttaga aactgtcgat agtgcaccgg tacaggcgct acgtgagaag
    55081 atgggcgagt ttgccgagct gcgcgatctg ctggagcgag caatcatcga cacaccgcca
    55141 gtgctggtac gcgacggtgg tgttatcgca tcgggctata acgaagagct ggatgagtgg
    55201 cgcgcgctgg ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa
    55261 cgtaccggcc tggacacgct gaaagttggc tttaatgcgg tgcacggcta ctacattcaa
    55321 atcagccgtg ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa
    55381 aacgccgagc gctacatcat tccagagcta aaagagtacg aagataaagt tctcacctca
    55441 aaaggcaaag cactggcact ggaaaaacag ctttatgaag agctgttcga cctgctgttg
    55501 ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtt
    55561 aacctggcgg aacgggccta taccctgaac tacacctgcc cgaccttcat tgataaaccg
    55621 ggcattcgca ttaccgaagg tcgccatccg gtagttgaac aagtactgaa tgagccattt
    55681 atcgctaacc cgctgaatct gtcgccgcag cgccgtatgt tgattattac cggcccgaac
    55741 atgggcggta aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc
    55801 ggcagctatg taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc
    55861 gtaggcgcgg cagatgacct ggcttccggg cgctcaacct ttatggtgga gatgactgaa
    55921 accgccaata ttttacataa cgccaccgaa tacagtctgg tgttaatgga tgagatcggg
    55981 cgtggaacgt ccacctacga tggtctgtcg ctggcgtggg cgtgcgcaga aaatctggcg
    56041 aataagatta aggcattgac gctgtttgcc acccactatt tcgagctgac ccagctaccg
    56101 gagaaaatgg aaggcgtcgc caacgtgcat ctcgatgcgc tggagcacgg agacaccatt
    56161 gcctttatgc atagcgtgca ggatggcgcg gcgagcaaaa gctacggcct ggcggttgca
    56221 gctctggcag gcgtgccaaa agaggtgatt aagcgcgcac ggcaaaaact gcgtgagctg
    56281 gaaagcattt cgccgaacgc cgccgctacg caagtggatg gtacgcaaat gtctttgctg
    56341 tcagtgccag aagaaacttc gcctgcggtc gaagcactgg aaaatctcga cccggattca
    56401 ctgactccgc gtcaggcgct ggagtggatt tatcgcttga agagtctggt gtaataacaa
    56461 ttcccgatag tcttttgcta tcgggaatat taacgacaac tgacgaatca aataaaaata
    56521 ccctgtataa taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa
    56581 aaaatcaata cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat
    56641 cagttattac aatcccgctt acatcaactc tcttttttcc ccgaaaccga cttacttatt
    56701 tctgtcggcg ataatattga tcgtgggccg gagagtcttg acgtcctgcg cctgctaaac
    56761 cagccctggt ttatctccgt taaaggcaac cacgaagcaa tggcgctgga tgcgttcgcg
    56821 accggcgatg gcaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat
    56881 tcagagcaaa aagaagctac agatctgttg ctgaaattcc atcaccttcc acatattatt
    56941 gaaatcacta acgacaacat aaaatatgtc atcgcacatg cagattatcc ggggagtgaa
    57001 tatctctttg gtaaagaaat agcggagagc gaattactct ggcctgttga tcgtgtgcag
    57061 aaatcgctta atggcgagtt acaacaaata aacggcgctg attattttat atttggacat
    57121 atgatgtttg ataacattca gacgttcgct aaccagattt atattgatac cggcacgccg
    57181 aaaagcgggc ggctgtcatt ttataaaata aagtagtctc atgcttcttc tgtgaagcat
    57241 gagtaaccaa atgttattgc aggccattat tcatttttcg ctaccagcaa agagagatcc
    57301 tgcttcgcca gcgcgcgact ggcactctcc ggcaaaccgt cgtctgtaat aatctgatca
    57361 aactcgctta atggtaacgc cagccatgtc gccacctgac catatttcgt cgcatcacag
    57421 accaaaactc gctggcggct ggcactggca atcgcccgtt tcaccgtgac tttatcttcc
    57481 gctggcgtag aaatcccccg cacactccat gacgatgcag aaataaaagc ctgatcaatc
    57541 atcaggctgc gcagcatggt cgcagcggct tccccgacac aggaacggtt ttcccgacac
    57601 actgcaccgc cagtgtgaat aattgtgcaa ttactgttgt cgagcaagta gttcgcaata
    57661 acgaaatcgt ttgtgaccac agtcagtgac tccatgtgaa tcagatgctg tgctatcgct
    57721 aacgtggtcg ttcccgcatc cagatagata caacttcccg gctgaacaag acttgccgcc
    57781 agcttgccaa tagccgcttt ttgcgtcatt gccagcgcag tttttacctg atgagaaggt
    57841 tcatgcgcca cgcgtcccgg agactggacg cctccggaca ccagcacaac ggctccctgc
    57901 tgctccagtt tttgtaaatc ccgacgaatg gtcatatgtg acacattcat tctgtccgtt
    57961 agttcagcaa tactgacaat gcctttttca gctaccatct caaggatgat ttggcgacgc
    58021 tctacgggta tcaacttttg ctccttcctt tgtcctgctg acattctacg ctatttgcct
    58081 gcgaaacgtg cgcggcgcaa ctaacgctta gttcacataa aataacacac aatgttaatt
    58141 tatgtgaatc agctcaccat atcgttatct tccagcgctt atattcacaa tatcaaacaa
    58201 aatatcactt aaattaacaa ggggagcaga tgaaaacggg atctgagttt catgtcggta
    58261 tcgttggctt agggtcaatg ggaatgggag cagcactgtc atgtgtccgc gcaggtcttt
    58321 ctacctgggg cgcagacctg aacagcaatg cctgcgctac gttgaaagag gcaggtgctt
    58381 gcggggtttc tgataacgcc gcgacgtttg ccgaaaaact ggacgctctg ctggtgctgg
    58441 tggtcaatgc ggcccaggtt aaacaggtgc tgtttggtga aacaggcgtt gcacaacatc
    58501 tgaaacccgg tacggcagta atggtttctt ccactatcgc tagtgctgat gcgcaagaaa
    58561 ttgctaccgc tctggctgga ttcgatctgg aaatgctgga tgcgccagtt tctggtggtg
    58621 cagtaaaagc cgctaacggt gaaatgactg tcatggcctc cggtagcgat attgcctttg
    58681 aacgactggc acccgtgctg gaagccgttg ccggaaaagt ttatcgcata ggtgcagaac
    58741 cgggactagg ttcgaccgta aaaattattc accagttgtt agcgggcgta catattgctg
    58801 ccggagccga agcgatggca cttgcagccc gtgcggggat cccgctggat gtgatgtatg
    58861 acgtcgtgac caatgccgcc ggaaattcct ggatgttcga aaaccggatg cgtcatgtgg
    58921 tggatggcga ttacaccccg cattcagccg tcgatatttt tgttaaggat cttggtctgg
    58981 ttgccgatac agccaaagcc ctgcacttcc cgctgccatt ggcctcaaca gcattgaata
    59041 tgttcaccag cgccagtaac gcaggttacg ggaaagaaga cgatagcgca gttatcaaga
    59101 ttttctctgg catcactcta ccgggagcga aatcatgatc aagattggcg ttatcgccga
    59161 tgattttacc ggcgcgacgg atatcgccag ttttctggtg gaaaacggtc taccaacggt
    59221 acaaattaac ggtgttccaa caggtaaaat gccggaagca atcgacgcac tggtgatcag
    59281 cctgaaaacg cgctcctgtc cggtggttga agccacacag caatcgctgg cggctctgag
    59341 ctggttgcaa cagcaaggtt gcaaacagat ctatttcaaa tactgctcta ctttcgacag
    59401 tacggcgaaa ggtaatatcg gcccggttac cgatgcatta atggatgctc tcgacacgcc
    59461 gtttacggtc ttctctccgg ccctgccggt caacggacgt acggtttatc aggggtattt
    59521 gttcgtaatg aatcaactgc tggccgaatc cgggatgcgc catcatccgg taaatcccat
    59581 gaccgacagc tatcttcccc gtctggttga agcgcaatcc acagggcgct gcggcgtcgt
    59641 ttcggcacat gttttcgaac aaggtgtgga tgccgttcgt caagagctgg ctcgcttaca
    59701 gcaagagggc taccgctacg cggtgcttga tgcgctgacc gaacaccatc tggaaattca
    59761 gggagaagcc ttgcgcgatg ccccactggt aacgggcggt tctggtctgg cgattggcct
    59821 ggcccggcag tgggcgcaag aaaacggtaa ccaggctcgc gaagcagggc atccgctcgc
    59881 tgggcgcggc gtagtgctct ccggttcatg ctctcaaatg accaaccgcc aggtggcaca
    59941 ttaccgtcaa attgcaccag cccgtgaagt tgatgtggca cgctgcctct caaccgaaac
    60001 tctggccgct tatgcacacg aactggcaga gtgggttctg ggccaggaaa gtgtacttgc
    60061 tccactggtt tttgccaccg ccagcactga cgcattggca gcaattcaac agcaatacgg
    60121 tgcacaaaaa gccagtcagg cagtagaaac tctattttct caactagcgg cgcggttagc
    60181 agcggaaggc gtgacacgct ttattgtcgc aggcggtgag acctccggcg tagtcacaca
    60241 gagcctgggc ataaaagggt ttcatattgg cccaaccatt tcccccggcg tgccgtgggt
    60301 aaacgcactg gataagcctg tctcactcgc ccttaaatct ggcaacttcg gtgatgaagc
    60361 ctttttttca cgagcccaaa gagagttttt atcatgagcg atttcgcaaa agtagagcag
    60421 tctttgcgag aggagatgac gcggattgcc agttcattct ttcagcgcgg ctatgcaacc
    60481 ggttcggctg gcaatctgtc gctgctttta cctgacggga atttactggc gacaccgaca
    60541 ggttcatgcc tgggcaatct cgatccgcag cggctttcca aagtcgccgc ggatggcgaa
    60601 tggttaagtg gtgacaaacc ctcgaaagag gtgctctttc atctggcgct gtatcgcaac
    60661 aatccgcgct gtaaagcggt ggtgcatttg cacagcacat ggtcgacggc gctttcctgc
    60721 ctgcaagggc tggacagcag caacgttatt cgtccgttca caccatacgt ggtgatgcgg
    60781 atgggaaatg tcccgctggt gccttattac cgaccgggcg ataaacgcat cgcacaggat
    60841 ctggcggaac tggcagcaga caatcaggct tttttactgg caaatcatgg cccagtggtt
    60901 tgcggtgaaa gcctgcaaga agccgccaac aatatggaag agctggagga aacggcaaag
    60961 ctgattttta ttctcggtga ccgcccgatc cgttatctga ccgcaggtga aattgcggaa
    61021 ttaaggagtt aatgcaatgc ctcgttttgc agctaattta tccatgatgt tcaccgaagt
    61081 gccttttatt gaacgcttcg ccgcagcgcg aaaagccggg ttcgatgctg tggaatttct
    61141 gtttccctat gactactcca ccctgcaaat ccaaaagcaa ctggagcaaa accatctgac
    61201 actggcgctg ttcaataccg ctcccggaga tattaatgcg ggggaatggg gattatccgc
    61261 ccttcccgga cgtgagcacg aagcacacgc agacatcgac ctggcactgg aatatgcatt
    61321 ggcgctcaat tgtgaacaag tccatgtgat ggcaggcgtc gtgcccgctg gcgaagacgc
    61381 tgaacggtac cgggcagtat ttatcgataa tctccgctac gctgccgacc gctttgcgct
    61441 acacggcaag cgaatattag ttgaagcact cagccccgac gttaagcctc attatctctt
    61501 ctccagccag tatcaggcac tggctattgt tgaagaggtt gcgcgagata acgtgtttat
    61561 tcaactagat acttttcatg cgcaaaaagt agatggcaac ctgacgcatt taatccgcga
    61621 ctacgctggg aaatatgcgc acgtacaaat tgccggacta cctgatcgac atgaaccgga
    61681 cgatggagaa atcaactatc cgtggctgtt ccgcctgttc gatgaggtgg gatatcaggg
    61741 ctggatcggt tgtgaatata aacctcgtgg cctcaccgaa gaagggcttg gctggtttga
    61801 cgcctggcgc taattcgtta aatccctaat tacaacgtac ccatacatcc ccccatctac
    61861 ggatggggta ggttaactat aactttcaga cagggtttcc catgtccaca attacattgt
    61921 tatgcattgc gttagctggc gtaatcatgc tgttgctgct ggtcatcaag gcaaaggtac
    61981 aaccattcgt tgctctgctc ctcgtcagcc tgttagtcgc acttgcggcg ggtataccgg
    62041 cgggcgaagt gggtaaagtg atgatcgccg ggatgggcgg tgtgcttggt tccgtcacta
    62101 ttattattgg tctgggcgct atgctggggc gtatgatcga acactctggt ggtgcagagt
    62161 cactggctaa ttatttcagt cgcaagttag gtgacaaacg aactatcgct gcgctgactc
    62221 tggcagcgtt cttcctcggt attcccgtct tctttgatgt cggctttatt attcttgcgc
    62281 caatcattta cggttttgcc aaggttgcca aaatatcgcc actcaaattt ggcctgcctg
    62341 tcgctggaat catgctaact gttcatgtgg cggtaccgcc gcatccaggc cctgtcgccg
    62401 cagcggggtt actccacgca gacatcggct ggctaaccat catcggtatt gcgatttcta
    62461 ttcccgtagg gattgttggc tactttgcag cgaaaataat caatacgcgc caatatgcga
    62521 tgtcggtgga agtactggaa cagatgcaac tggctccggc cagtgaggaa ggcgcaacaa
    62581 aattaagcga taaaataaat ccaccgggcg tcgcgctggt cacctcgcta attgttattc
    62641 ctatcgcgat tatcatggcg ggtacggttt ccgcaacact gatgccgcct tcgcatcccc
    62701 tgcttggtac gctacagctg atcggctcac caatggtagc tctaatgatt gcgctggtgc
    62761 tggcattctg gttattggct ttacgtcgcg gctggagctt acaacacacc agcgacatta
    62821 tgggctcagc ccttcccact gcggcggtag tgattttggt tactggtgct ggaggggtat
    62881 ttggcaaagt gctggtggaa tcgggcgtcg gcaaagccct tgccaatatg ctacaaatga
    62941 ttgacctgcc tctgttacca gccgcattta ttatttcact ggctctgcgt gcatcgcagg
    63001 ggtcagcaac cgtagcaatc ctgacaaccg gcgggttact ctcagaagcg gtgatgggat
    63061 tgaatccgat tcagtgcgta ttggtgacgc tggcagcctg ctttggtggg cttggcgcgt
    63121 cacatattaa tgactcaggg ttctggattg tgaccaaata tctggggttg tcggtagcag
    63181 acggtctgaa aacctggaca gtgttaacga ccattctcgg ttttaccgga ttcttaatta
    63241 cctggtgcgt atgggcggta atttgacctt aataaaaagg tccgatgggc atcggacctt
    63301 ttatttaacg cttaccttcc accagcagcg gcgggatgct cggcaccatt ggcgcgtcat
    63361 caatatcttt ctgcgtcatg cggaacgctt cgggataatg ttcgcggctg gtacggcgca
    63421 gcgattcggt atcgcgccag gtataaaggc aatgctggca ctgatatacc gtccagacat
    63481 ctttcaccgg cgatttcgcc atcacttcaa tctgttcatc ggcacaacgt ggacaaatca
    63541 tcttcttctc cttatttacg tgcagccagc atagcggtca gtttttcagc ccaggctttg
    63601 gtttccggta aatccaccac cggttggctg tagtgaccac ggttgtccgg ggcgacaggc
    63661 gtagtggcgt cgataatcag cttgtcggtg atccccgcag ggcttgagcc tggatcgagt
    63721 tccagcacgg acatattcgg caactgcacc aaatcccctg ccgggttcac tttcgaggag
    63781 agcgcccaca tcacctgcgg caggttgaac gggtcaacgt cttcatcgac cataatcacc
    63841 atcttcacgt agcccagacc atgcggcgtg gtcattgcgc gcaggcccac cgcgcgggca
    63901 aagccgccgt agcgtttttt ggtggagata atcgccagca ggccatgggt gtacatggcg
    63961 tttaccgcct gcacttcagg gaactcggct ttcagctgct gatacagcgg cacgcaggtg
    64021 gctggcccca tcaggtagtc gatttcggtc cacggcatac cgaggtacag cgattcgaaa
    64081 atcggcctgg tgcggtaaga gactttatcg atgcgcacca cggtcatgtt acgcccgccg
    64141 gagtagtgcc cggtaaactc accgaacggc ccttcgattt cgcgtttacg gctttcgatg
    64201 accccttcga ggatcacttc tgaaccccac ggcacatcaa aaccggtcaa cggggcggtg
    64261 gcgatcgggt acgggctttc acgcagcgcg cctgccattt cgtactcgga ctgatcatat
    64321 ttcagcggcg tggcccccat cagcgtgatg atcggatcgt taccgagcgt gatcgcaatc
    64381 ggcagatctt caccgcgctc ttctgcttta tgcagatgca gggcgatatc gtgcatcggc
    64441 accggttgca ggccgagctt acgcttgccc ttcacttcca tgcggtagat gccgacgttc
    64501 tgcttgccga agttatccgg gtcgagcgga tcgcgggaaa ccacgcacgc tttgtcgaga
    64561 tagaaaccgc catcgccatc gtttaaacga aacagcggca ggatatcgaa caggttgatc
    64621 tcgtcgccat caacggtgtt ctgcgcccag gctggattgg cgcggcgctc cggggcaatc
    64681 gggaagttat cccagcggcg gataaactca tcaatctgct ttttaaccgg ggcatttggc
    64741 ggcaggccga gggaaatcgc gtggttctgc caggaaccga tggtgttcat cgccacgcgg
    64801 gcatcggtaa agccgcgaat attatcaaac cacagcgcgg gcgcgccgtc gccgatacgc
    64861 ccggtggcgt tagctgctgc tgccagatcc ggctcggcgt tcacttcttc gctgattttc
    64921 agtaactggc cgtggtcatc aagcgcctgt aaaaagctgc gtaaatcatc aaatgccatt
    64981 attcattctc ctgagaaaaa ttccgggcct gcggcaatcc ttgccagcgc ctggcgtggg
    65041 gatgttcgag gccaaattga tccagcacgc gggctaccac atggtggaca atgtcatcta
    65101 ccgtttcggg atggttataa aaggcaggca tcggcggcac catcgccacg cccatgcgtg
    65161 aaagtgcgag catattttcg agatggatgg tgctaagcgg catttcacgc ggcaccagca
    65221 ccagtttgcg gccttctttg agcacgacgt ccgccgcgcg ccctaccagg ccatcggcgt
    65281 aaccagcgcg gataccggcg agcgttttca tactgcacgg aataacgatc atgccgtcgg
    65341 tacgaaaaga accggatgag atgatcgccg cctgatccgc cgggttatgg ctgaagtctg
    65401 cgagggcagc aacatcgcga gcgctgtaag gcgtttccag ttcaatggtg gttttcgccc
    65461 acttcgacat caccagatga gtctcgacat tcggcatctc ccgcagcgct tgcagtaatg
    65521 ccacaccaag aggcgcaccg gtagcccctg tcatcccgac gatcagtttc attgcttatc
    65581 ctcttaattg ttcgtatacg aacgttatta ttaacatact ccttcatttc cacagcggca
    65641 aggattgaaa agtaacaaca tcttcttcgt cactaaaaaa ccgctatgat agcgacagag
    65701 aattagcccg gagtttacat ggcgttacga aataaagcgt tccatcagtt acgacagctt
    65761 tttcagcagc acacggctcg ctggcagcac gagttacctg acctgaccaa accacagtac
    65821 gcggtgatgc gcgccattgc tgataaacct ggtatcgaac aggtggcact gatagaagca
    65881 gctgtcagca ccaaagcaac gttagcagaa atgttggcaa gaatggaaaa tcgtggctta
    65941 gtcagacgtg aacatgaccc cgctgataag cggcgacgct ttgtctggtt gactgccgaa
    66001 ggcgaaaaga tacttgctgc ggcgataccc attggtgaca gcgtggatga ggaatttttg
    66061 gggcgtttga gtggagaaga gcaggagctg tttatgcagc tggtgcgcaa gatgatgaac
    66121 gcataggggg caaatttccg gatgcgacgc tatcgcgtct tatccagact acaaatacac
    66181 cgactcacca accgaaagaa aaaaggccag cctcgcttga gactggcctt tctgacagat
    66241 gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca ggatttcgcg
    66301 caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga ggccaatttc
    66361 acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag
    66421 cacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct gcttcatatc
    66481 gtcatcttgc gtggtatctt ccggaccgtt ctctttttca tcggccagga tgtccagcaa
    66541 cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct cgttaagacg
    66601 aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct cttccgcact
    66661 tggttcatgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc
    66721 ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg cccgttcaat
    66781 cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa
    66841 cttctctacc gcgcggatca gccccaggtt gccctcttcg ataaggtcca gcaacgccag
    66901 accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac tctcgatcat
    66961 ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc
    67021 ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg cgtccaacac
    67081 acgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat cactgggttc
    67141 ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc
    67201 ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc tacccgtgat
    67261 cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg cggcaaataa
    67321 cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg
    67381 gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg ttgttcccgg
    67441 accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg tttgatgata
    67501 atcagattac cgtagccgcg cagcgcgtta ccggcataaa caacgcggcc atctgcggtc
    67561 gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa
    67621 gcgccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga gataggcgta
    67681 ctggttgatg tactgctgac agttggctcg gttgtgcttg ctgttggtac cgttacaggc
    67741 gctgtgaccg tggtcgcagc tggcttgttg ttcggcaaca ttttgttagc actctgttca
    67801 cccgaagatt cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt
    67861 gcaggcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatggc atttccgcca
    67921 gtgattggcg taccggaagc attacccacc tgcaaggtct gaccaacgtt cagcgcgtat
    67981 ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt aatccaggcg
    68041 atatagaaaa gtgtgtcgcc ttttttcacg gtataggtac tgccgctata actgcctttc
    68101 ggaatgttcc catactgacg gttatagacg atgcgtccgt tttccatctg taccggctgc
    68161 tgagctactg gctgcactgg ctggatttgc ggttgttgag tagcctgaat ttgtggctgc
    68221 tgtaccggct gaatttgcgg ttgctgggct gtagacgtcg tccccatttt cggcggcggc
    68281 gtaatcaaca taccagaatt ggtatttgca ggcgcattgc cattaacgga gctgaccggt
    68341 gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag tgacaaagcc
    68401 gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc aggaaaaatt
    68461 ggttaataac cagtgacata attaccgtgc aaggcaccct actgaacact ggaaaagatg
    68521 ttcacgatac gctgacctgc ggcaaaataa ccaggaaaaa tccaggtatt tcctcacgtt
    68581 ttaagccagc tcacccttca ctaaaggtac aaagcgcacg gcctccacgg tatcgataat
    68641 aaattcgcct ccccgacgac gcacccgttt caaatactgg tgctcctccc ctacgggtaa
    68701 gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt ccggcggtgc
    68761 cgccgtaaca atgatagcgt caaacggcgc gcgtgcctgc caaccttgcc atccatcgcc
    68821 atgacgggtt gaaacattat gtaaatcaag atttttcagg cggcgacgcg cctgccactg
    68881 caaaccttta atccgttcga ccgagcaaac atgctggaca agatgcgcca ggattgccgt
    68941 ttgatatccc gaaccagtgc caatttccag cacccgcgac tgcggcgtca gctcgagtaa
    69001 ttcggtcatt cgcgccacca tatacggctg cgaaattgtc tgcccctgac ctatcggcaa
    69061 ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt cacgcggcac
    69121 ggcggcaagt gcattcagca cctgctcatc ctgaatacct tgcgcacgta attgatccag
    69181 aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt taaccagtct
    69241 gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt gatggagaca
    69301 tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt accgcccggc
    69361 gggccaatcc agtacagcgt attgccgcgc ggatcttgct gcgggatcac ctgatctgcc
    69421 ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa gggtaaatcc
    69481 ggaacgttaa tattaagaat acgcccggtg cgcaacggct ctttacacag tgcgcgcaaa
    69541 attgaacagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag cgagacggca
    69601 agcgccggaa aacctaaatg acggccttcc atcgcggcgg ctaccgtacc ggaataaata
    69661 acatcatccc ccagattcgg cccggcgtta attccggaca caacaatgtc cgggcgcgga
    69721 cgcatcagag cattcacgcc aagatagacg caatcggtcg gggttcccat ttgcacagca
    69781 atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag agaatttgaa
    69841 gcgccgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc acgcaaggct
    69901 ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag caatatgcgc
    69961 ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac tacccgccgg
    70021 aagccagaaa cggatctcta cggtgacgtc atcccaccaa ttccagctta attgttgcgg
    70081 atacagcagc atcgctctgc gcgcggcttc aactttttcg cgcaccagta aagcttgtaa
    70141 ttcagtttct gcggcgacag ctgcttgttc gaatgccagc gcttcacgct gagttcccca
    70201 ttcgccactg cccggcaatg cggcggttat catcagctct ttatcgttaa cgcgacgctg
    70261 taattccgcc agttcttcgg tggttgcgac aaaccagcta ccacgtccgg ctaattgtag
    70321 cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgctcag caacaatctg
    70381 attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc gatcgcgcac
    70441 cggagtattg gtttgcgccc agcgctgcgc cccctgcaag ttgctaccgc caatcccaaa
    70501 acgttgggca ccgaagtagt tcggtacacc ttttacgcaa atatcgttca gacgttgttc
    70561 aacgtcatcg cgattgctca cttcgcgcag aaccagggta aaggcgtttc ctttcagcgc
    70621 ccctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc cttccagttg
    70681 aaaggcgctc agatcgggca tttccttgcc cggcacgcga gcgcataacc actgttccgt
    70741 aacagcatgt ttgtcttttt gcccagcgaa gctgacttca cgggcatgaa ttttcaggaa
    70801 tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga ttctaaccag
    70861 aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca caaagtcttc
    70921 cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag tgagattatc
    70981 aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc aatcccttcc
    71041 ccacgtccgg taaatcccag tttttccgta gtagtggctt tcacgttaac atcatccata
    71101 tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg caacatcttc
    71161 ggtgcctgag cgatgatagt gacatcgacg ttgccaaggg tataaccctt cgcctgaata
    71221 cgacgccagg cttcgcgtag cagctcgcgg ctatcggcac ctttaaatgc cggatcggta
    71281 tccgggaaca gcttgccgat atcccccagc gccgccgcgc caagcaatgc atcggtcaac
    71341 gcatggagcg ccacgtcgcc atcagaatgc gccagcaatc ctttttcgta aggaatgcgt
    71401 acgccaccaa tgataattgg gccttcaccg ccaaaggcat gtacgtcaaa accgtgtcca
    71461 attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaactcg gccagtgcca
    71521 aatcttccgg gcgcgtgact ttaatgttat ccgcacggcc ttcgaccaac tgaggatgga
    71581 atccgcaata ttccagcgcc gaggcttcgt cggtaatagt cgcgccttca tttagagcgc
    71641 gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc gcgtgccata
    71701 agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca cgtttcatag
    71761 tatcgcgcac tggtgcggcg aggatccccc ccgtgcggct ggtttcgctc aacgccaaca
    71821 atcgcgcgag gtcatcctga tgcaaacaag gacgagcggc gtcatgcacc aatacccact
    71881 gcgcgtcgcc agcggctttc agacctgcca gcacggaatc ggcacgctca tcaccgccat
    71941 ctacaacggt gatttgcgga tgattcgcca gaggaagttg tgcaaaacgg ctatcgccag
    72001 gacttatggc aatgacgact cgtttcaccc ggggatgcgc cagcagcgca tgcaccgagt
    72061 gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc gtttgcattc
    72121 gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg gttgccatgt
    72181 taattcccgg gctgatttat cgattgtttt gccccgcaga ctgtgcgcgc ttcgacgcgt
    72241 caggcaccag acgataaaaa gtttcgcccg gcctggtcat gctgagttca ttacgcgcac
    72301 gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa agttgatcgt
    72361 ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc cacatcatca ttgacgcggg
    72421 tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag accagaatag
    72481 ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaacggcat catcatccca
    72541 tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc gggcaaatgt
    72601 accacatttg tccattgtta cgtataccca gggcgtgcag aacataatct cattattagt
    72661 tacggtttga attatgaaca gaggagacgg gaaagtacaa attagcccag tagccacata
    72721 aacagtgcgc caaacataat gcctactgtc accagggtga aaacaatact gtagcgtagc
    72781 tttccgtcca tcaatgaatg cagcgcaatc cccaccacta ccgcgacggg catcagcgcc
    72841 agaaagaaag gccaggtgta gataaagaag aacagcgtgt tagagccata aatcaacatc
    72901 ggcatcgcca gcgcaaataa ccaggagata aaaccgacca cggcaccagg cagtgaccat
    72961 gtggtttctt catcctcagt aaggctgtcg ttatttgtta gtgtaatgtt atggctatta
    73021 cgcatatttg atcctgttac tttgacgaac cgggcatgga aacccggtgg tgtctcagga
    73081 tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat ttgttactaa
    73141 ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg aatctattcc
    73201 cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aagccttttg gatcgcgggc
    73261 ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt ctcctacgcg
    73321 ttcgcgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca gcactaccag
    73381 tccggcttca accatcaaat tcgccacttc accgacgcga cggatattct ctttacgatc
    73441 ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acattatcgc catccagcag
    73501 atacgtactg acgccgagtt tatgtaacgc ctcctccagc gccccggcga ccgttgattt
    73561 accggacccg gagaggccgg taaaccacag cactacacca cgatgaccgt ggtgtagctc
    73621 gcgttgttgc acagtgaccg gatggctatg ccagacgacg ttttcgtcat gcagcgccat
    73681 tatttatccc ccagcaaatc gcgcgcgccc cagtgcggaa agtggcgacg aaccagagca
    73741 ttcaattcca gttcgaatgc actgaattca gatggcgcag cagttgcctg gctaactggc
    73801 tcgtgcacca taccggcacc cacggtcaca ttgctcaggc gatcgataaa aatcagccca
    73861 cccgtcaccg gattttgttg ataacgatct aacaccagcg gctcgtcaaa agtgagatcc
    73921 acgaggccga tcccattcag tggcaggttt tcaacttcac gctgggtaag gttattaata
    73981 tcaacctgat agcgaatgcc atcaacacgc gcgcgcgtct tcttaccggc aattttgatg
    74041 tcgtaactct gccctggaga aagcggctgt tccgccatcc ataccacatc caccgacgcg
    74101 ctctgcaccg ccggtaacgc ttcgtctgcc gccagcagca gatcgccacg gctgatgtcg
    74161 atctcatccg tcagcaccag ggtgatcgct tctccggcaa aggcttcttc gcgatcacca
    74221 tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcac tttgacacgt
    74281 tgcccgactt ccacgcgacc ggatgccagc gttccggcgt aaccacgaaa atcgagattc
    74341 gggcgattaa cgtactgcac cgggaagcgc attggctgag catccaccac tcgctggatc
    74401 tccacggttt ccagcacttc gagcagtgtc ggaccgctgt accacggcat actttcactt
    74461 tgcgatgcca cgttgtcgcc ttccagtgca gagagcggca caaagcggat atccagatta
    74521 cccggcagct gcccggcaaa ggtcaaataa tcttcacgaa tacgggtgaa cgtctcttca
    74581 ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgct ccccaacagt
    74641 gtggagataa aactgtgacg acgggtttga tcgagcacgc ctttacgggc atcgatcagt
    74701 aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgggtgta ctgctcgtgc
    74761 cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaatagcg gtaggccacg
    74821 tcaatggtga tgccctgttc gcgctcagct tgcaggccgt ccaccagcag agccagatcc
    74881 agcttttcgc cctgggtgcc gtgacgctta ctgtcgttat gcagcgatga gagctgatct
    74941 tcgtagattt ggcgggtatc gtgcagcaga cgaccaatca gagtactttt gccgtcatcg
    75001 acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat catccaggct
    75061 tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt aaaaataccc
    75121 ctgacgtttt ttcagctcca tagaccccgc ctggtcgcgg tcaatcacgc ggccctgacg
    75181 ttcactggtg gtggaaacca gcatctcttc gatgatttcc ggcagtgttt gtgcatttga
    75241 ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg aaacgcacca tccgtttttt
    75301 aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca acataccgtc
    75361 gcgttccaga accggacgtt ccgcagcaag atatagcgga acaatgtcga tattttccag
    75421 ccagatgtat tgccagatat cctgctcggt ccagttagag agcgggaaga cgcggatgct
    75481 ttcgcctttg ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct gatttttcgg
    75541 atcccagcga tggaagcggt cacggaaaga gtaaattcgc tctttagcgc gggatttctc
    75601 ttcgtcacgg cgcgcaccac cgaaggcggc atcaaaaccg tatttgttca gcgcctgttt
    75661 caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgcacga atggattaat
    75721 ccccatcgcc acgccttccg ggtttttatg caccagcagt tcgcagccgt aggctttagc
    75781 agtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcga catgcagcaa
    75841 cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca tgacgctgga
    75901 atctttaccg atagagtaga gcatcaccgg atttgagaat tctgccgcca cctcgcgaat
    75961 aatgtggatg ctttccgcct ccagttgccg caggtgagta agtcgtattt gatccataac
    76021 cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata gggagcgtaa
    76081 gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa cgatttggca
    76141 aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa gatttgaaat
    76201 tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat ggtatttaag
    76261 gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc ggcgtatgct
    76321 ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat actcaggcac
    76381 gacatattgc tactttcttt ccgggacgca tgaccggaac tcctgcagaa atgttatctg
    76441 ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt cggacattta
    76501 atagtcggta tatttatacc gcccgcgata atcgcaagag ctggcataac gtgacgggaa
    76561 gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcatcatt atggcgcatc
    76621 tggatactta cgccccgctg agcgatgctg acgccgatgc caatctcggc gggcttacgt
    76681 tacaaggaat ggatgataac gccgcaggtt taggtgtcat gctggaattg gcagaacgcc
    76741 tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc gaagaggaag
    76801 ggaaattagg cgcagagaat ttactcaagc ggatgagtga caccgaaaag aaaaatacgc
    76861 tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc aacagcggtg
    76921 taaaaacccc tgaagcagta aggaaattaa cgcgcgacag ggcgctggca attgcgcgta
    76981 gtcatggaat tgccgcaacg accaatccgg gtttgaataa aaattatccg aaaggcactg
    77041 ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt tcggtggaag
    77101 cgactaactg gaatcttggg aataaggatg gttatcagca acgcgcaaaa acacctgcct
    77161 tcccggcggg aaatagctgg catgacgtaa gactggataa tcaacaacat attgataagg
    77221 cacttcctgg aagaatagaa cgtcgctgcc gtgatgttat gcggataatg ctaccgctgg
    77281 tgaaggagct ggcgaaggcg tcttgatggg tttgaaaatg ggagctggga gttctaccgc
    77341 aggggcgggg aattctaagt gatatccatc atcgcatcca gtgcgcccgg tttatccccg
    77401 ctgacgcggg gaacacaaaa ccaaacttct ccataaattc catagccgcg gtttatcccc
    77461 gctggcgcgg ggaacacgag tctatcagcg acactaccgg caatagcgac ggtttatccc
    77521 cgctggcgcg gggaacactc ttcgcgggta atcaatgatg attcagtttc cggtttatcc
    77581 ccgctggcgc ggggaacacg aacgcaatat cacggcgttc tgcggcctcg gcggtttatc
    77641 cccgctggcg cggggaacac caaaatatta cgagcttcgt caggccatgg accggtttat
    77701 ccccgctggc gcggggaaca cgtcgggccg ggtaagtgaa cctgtcggtt ttccggttta
    77761 tccccgctgg cgcggggaac acactaagca tacataactg tttttaaaca aatttattcc
    77821 gcatcaacaa tctaccaact aaattaaaac atttccttat ttttaaagaa cggataactt
    77881 attgattatc aacaggaaga aaagaaacca aacgtaaccc atccaaatcc accggaatac
    77941 gtctgttttc tccccaggtc tgaaattcaa aacccgactc ggtattggtc gcccaggcca
    78001 tcaccacatt tccgcaacca gccagttggg taatttgctg ccagatcatc tcccgaatac
    78061 gttttgatgt atcaccaaca tacacaccgg cacgcacttc cagtagccag attgcgagcc
    78121 gtccacgtaa gcgcggcggg acattttctg taacaaccac gaccatgctc atcagccgcg
    78181 tcctcgatga ccgctatcgc ccaacgtttc aggttcgggg atggctggcg gtaacatatc
    78241 cggcgcaggt tgtggcggtt caatttcacc tgcggcaagg acttcctcaa ttaacggtat
    78301 taatttgccc gttaacttag tgctacggaa aatatcgcga caggctaatc tgacttcttt
    78361 atcaggttct gcgggttgcc tcgctgctat ttcaaatgcc tttggcacaa ccgaatcaaa
    78421 tttaatgata tcggctatgt cataaacaaa tgaaagcggt ttgccactat ggataaaccc
    78481 aatagcgggc gcatatcccg cggctaatac tgccgcttca gatataccgt acagacatga
    78541 tgtagcagca ctgatgcagc gattcacaac atcgcctttt tcccagtctt taggatcgta
    78601 tttgcgacca ttccatttca caccatattg tttcgccagt aatgcatacg tctggcgaac
    78661 gcgagatccc tcaattccac gtaactgctc cactgaacgg cgagctggct gtggttcacg
    78721 aaaacgtaat tcatacattt tgcgcaccac cttcaaccgt agatcttccg ttaaagccag
    78781 ctttgcctgg tagagtaatt tatctgcccg cgccccaccg ggttgtccgg aggagtaaac
    78841 gcgaacgccc gcttcaccga cccagaccag cagtgttccc accgtggcgg ccagatgcac
    78901 cgccgcgtgg gaaactctcg ttcctggttc gagcataatg caggcgaccg atcccaccgg
    78961 aatatgcgtt cggatccctg ttttgtcgat cagcacgaaa gcgccgtcca gtacgtcgat
    79021 ctgaccgtac tggaggaaga tcatggaggt gcgatctttt aatgggatcg ggctcagtgg
    79081 tacaaacgtc acgcctctgc tccgggtttg atcagcatca gaccacagcc gaacgcccgg
    79141 cttttaccat acccctggct caggcgttgc acaaataacc caggatcggt gaccgtaagc
    79201 atccccgtat aatccacgct actgaactgg atcagttgcc gggagttttc ccgccgcagt
    79261 tgctgttgtc ggtaggcatc aaccgaggta tccagcagtg taaaaccgct tctctccccc
    79321 tgcgctgcca gccagtccag cgccgcctgt tgttgatgca accagacatc acttccttcc
    79381 gccggccccc tcacctgccg tttcgcctcc atcagcagat cgtggcgctt gcccgcttta
    79441 cagattgttg gattcgcccg caggttgaaa caaagttgtt gtccggtaca cagctcgggc
    79501 gcaaatgacc ggcattctat ggtgaacgta tcactttccg ccgggcgttc ctgcgacagt
    79561 acaaaaaagc gaaacgcgcc ctggagttct tcccgacgat aaagaaattg cctttctttg
    79621 ccgccaggga agagatccca cagccactga tgcatcacat attcaccgcg atccaccaga
    79681 tgcagcaact gcgcaggcga aagctggccg gtatgtaagg ttattcttga gaggtacatg
    79741 gttcctcctt gctgagccac ggcccctgat tgacggtgcg ttccccaaac agccactgct
    79801 gacgatttaa aggaacatcc cggcggcgta atattttgct cgcgaccagg ccgtcgtgtt
    79861 tcccttccca ccagcattca tcctgaagtt tcgggagtga gactttcagt ttatggaaat
    79921 gatcctgata ctgttggtat gcattacata aggcatcaca cgcgttgcct tcgagcagta
    79981 acggcgcaag aggtaatgcc agcggatgac tttttcgccc cagataaagc ggaaaaactg
    80041 gatggcgtaa accgtcctgc aactgttcga ggctgtaagg cgcatcggct gttgttgcca
    80101 ccgccaccat ccaccaggca tcggtgtagt agtcgcgccg ggagataatc gcgctcaaaa
    80161 gctcagggtc gctcaactct tcgcgacggc tgaaataacg tgctttacgc acctcttttg
    80221 gcatctggac cgtgtgataa tcccgtgccc agcgcgggtt acggctggcg caaaccacca
    80281 gtgaatagtg gcgattaaac gcgtttaagc gttcggtatc atcacgccga atccctaccc
    80341 cggcagccag tagccccagc aatgctgagc gcgaaggaag ttcatgggta tgacgaactt
    80401 cacctggagc atcgcccccc caggacgcca tcggcccatg aagctgaaaa atcagatatt
    80461 ggctcataag ccgcccctta cgcgcagata aagtccagca cgtccttcat gcttccctgt
    80521 ttgttcatca cgtcaaaact tgcgcattcg gtcttctgtt cgtagaccgt attcatgttt
    80581 tcgcgaagcg ttgtaatacg ctgcaccgcc acatctaact gacgtgtgcc attaatgggt
    80641 tcatagaaag ccgccgccag agagcgtggt tgatcggtgc ctttttctgc cagcgcccag
    80701 gaggcgtagg cacggctggc aaagctgttc tgtttgccgg ttggggagac tttaagtgcg
    80761 gcttccgtaa aggcacgtat ggtctgatta gctaacgctt cgtcaccgcc aaggttttcg
    80821 accagcagat ctttatcaat gcagatatag gtgtagaaca gtgcagaacc gaatccggtt
    80881 tcaccaagat gcccggcacc ggcatcttca gaagcctggc gcaaatcatc aacggcggtg
    80941 aaaaaatcat cttcgacaat cgtttcactg acaccaaatg cgtgcgctac ctggcaggcg
    81001 gcttcaacat taaactcggg tttattcgcc agcatacgac caaacatggc aatatctacc
    81061 gccatacggt ctttgcgtaa caacgcgaga tcttcctctt ttggcgcgcg cttttcttct
    81121 gcaagctgat gggccagcgc ttttacggcg tcaaattctg ccgggctgat atggactaac
    81181 tgttcagttt cggcgttagt gagcggatct tttggttttt tgtcattttt agctttccca
    81241 agataatcag caatttttgc cgaccattcg atggcttttt tatcttcaat ccctttctca
    81301 atcaggatag ttgccgcctc acgcgcaata cgcccactgc gaataccaat atggcccgcc
    81361 agtgcctgtt caaaaagtgc agaagtgcgc cacgcacgtt tcagactttg cgaggaaacg
    81421 cgcagacgcg ttgctccacc cagcaccaca gtttttggcg caccggtatc atcacggtta
    81481 aggttggcag cagggtaagc ggttaacaaa tgaagctgaa taaatgttgt catgagatag
    81541 tcctttattg attttgttcg ttatcggcgt cgccagcctg gtaatattcc agcgctcagc
    81601 ggatacggat aaattcggtt ggccgctgct ggcggcggtg atgattgagc aggtcgtcgc
    81661 tctcctggca ccaacggaag accccctctg ccagagagtc aagattgacg gaaccgttta
    81721 ataatctgac tgcgcggcgt aactggcgca gtaattcatc cggtgttttt actgccgaca
    81781 gacgagtaaa acgccccttt gacattaccg ccgccagctg cgcggcaaaa ggctgccgtt
    81841 catcaatagc ttttacattg gcgctaactg cggcgaccag cgccagtgcg gtaaaacgcc
    81901 actcttgtgc ttcaattttc cacaaagtgt gggtctgcat cagcagagaa cgaaaccctt
    81961 ctgacaagca aacatcatta accgttgtgc tacgccgcag acttgcacga ttgccgcgtt
    82021 tagcctgcaa ctcttcgcgc catttacgca gcgtggcttt gtgctcctct tttacaatac
    82081 tcattcagca gcctcctgct ttttctccct ggcggcttta gcactttgct tctccgccga
    82141 tgttgtgaaa tatttcttgc gcgcggtcat aacgcgtttc aaatcaacag gctcatatgg
    82201 attggtgaat acacgctcgt caaaatcctg acgcgcgaat aaccaaattt ccttttgcca
    82261 tttgccgagt aattcgtccg catcctgacc ttcttcaatt tggcgcacta acctcaggaa
    82321 gcgatgctga gttttgttcc agaagtcgat atccacaaaa ctgaaatcac cccgtgcacc
    82381 ttttggatcg gagaaccacg cttctttcaa tgcacttcgt aacagactta gaatccgtga
    82441 agccgtttgc gcagccagac gcagtttcgg tatctggcct tcttttttct tgagcagcag
    82501 cgggaaatgg tgttcgtacc aacagcgcgc tttcatgttg tcgaaatcat agccaaatcc
    82561 ccacaggcct acttttgcct gtttcagact gctggcatta aagagtttca ccaccagcgc
    82621 gggaagttcc gtattgtttt ctgatttacc cgtttcgata aggcctaacc agtcgcgcca
    82681 gattaaaccg cccggttgtg gtttgaccga gtaaaactcg ccaccctctt taagtggtat
    82741 acggtaaggc gttaagggat gctgccacat ggcataattc gcaccgtaat ttttggtcgt
    82801 catcaggctc aggagcgcgt cgctctgttc accgcaaata tcgcagttgc cgactgtcgt
    82861 ggtattaaaa tcaatacgaa tacggcgcgg cattccccag taagcctgga gtttattgac
    82921 ctgatcatgg gttaccaccg caccggccag ttcgctggtt cgcgtcgggc caagccaggg
    82981 gaaaaccaga tcgtcaaatt ttttgggtag cggtaagtcg gcttcatcct gcggcatcac
    83041 attgggccac agtttgcgcc acaagggcgt ttgttgattg ccttgatatt cctgtaattc
    83101 aatcagagtg gtcatcggcc caccgccgcg caaaccggtg cgatagcctt tgccgcctga
    83161 tggcgcattt aactgtaggg agaacagagc taacgcggaa caatgagggc atacgtgttc
    83221 agtcacgcca cgcttaataa agtggtcttt attaaacttc gttgtttgag cgccgggaat
    83281 ctcaggcagt agcgaagcga cctgaacttt atctcccttg agcgcctcga aatcctgcat
    83341 aaatgaaggg gaatctgccc caaactggaa agcgtgttct aaggacagca atgcttcccg
    83401 tagcttttca gcctccagtc cgtcttgcca gatatcatcc caacgaccgt gatttttggg
    83461 cgcgaagctg gtttgtagta gccccagcaa aaactgccag gccgccccct ggagatctgc
    83521 ccgcggcgca gcgatatcga caacattttc atcagccaga tcgactggtg ccagcttgcc
    83581 tgtcgttccg tctttaaaac gaacaggcaa ccacggggtt gtcagaagtg aaaacgagtt
    83641 catacgcatc ctctcgtaaa aaagaccatc ccaggtcggt tgtccatctt tccatccttt
    83701 tcggtgtatc aactattcag agagtcctgt cgcagacatc tcaaccaacc ttgccaatca
    83761 atccttccgt agccgaatac ccgcaacttt cgtcatcgtt gactaaaatc acgtttgcca
    83821 tctccggatc ttgtcgctgt tcaatgcacc acagcctgaa cgcctctccc tccagtaaag
    83881 aaaactcatc ccgatgtttt ttccaccagc ttcggcgcac tctgacaacg ctcatttccc
    83941 atgcgtgagc accggtggca taaggcttca ccacaccgtc aatacaggta gccagccaca
    84001 gggaaacaga ctcctcagcc agacgtgtcg acagtttttc cgggaggtaa tcgttgatat
    84061 tggcggcata gccgggcctg aagttcagca caaacttttt agccattgcg cgatcgcagt
    84121 aatatttgcc cacttgctcc tgctcgctgc gggcaaatcc ttccggcatt accacgtcct
    84181 caccgtagac ggattcaata agaagccggg ctgcgtgtgg catctgaata gcaccttgct
    84241 cacgcagcac acgctgcgtc agccagattc gcccatgatc gggatagaca aatgcactgt
    84301 tacgcatggc actgccaaac cattcgtcgc caggagagtc gtcccagacg ggagccagaa
    84361 tcagcaattc agggggggaa cgctcgtcct ttccgtcacg ctttaactga ccattaatat
    84421 cgcggatatg ccgctgtaat cgtcctgctc gctgaatcag caaatcaaca ggggccaggt
    84481 cggagatcat ttcgtccagg tcaatatcca cgctttgctc aattacttgt gtacaaacaa
    84541 tgacttgcga cgctctctgt aaactacaat atttaccaaa gcgtgccagc gtttccgttt
    84601 caattcgctg gcgatcgcta aaagcaaagc ggctatgaaa aagtgaaagg ctggaagcag
    84661 gaatgacgcc gcgggcaagc agctgacgat gaaccttaat agcgtcatcg acagaattcc
    84721 ggatccaggc gatgcatttc ccctgactta ccgccgattc gatacgcgca atacactctt
    84781 gttcactatg aagccaaccc acgctgacgc tacgctcaac atctttgcgc gtcgctaccc
    84841 ggtgtgagtt cacatcggat ttcgtgacat gcgtcagcca ggggtaatca tccttttcca
    84901 ggaacggagc ttcttgctgg ccctctgtac cccgcgcaaa ggcagcgacg agtttgtcgc
    84961 gctgctgttg agataacgtg gcagaaagca aaataacgct gtttccgcca cgcgcctgcc
    85021 gctcgatcaa cccttcaaga atgcacgaca tgtaagcgtc acaggcgtgg atctcatcag
    85081 ccagcaggat tttgttactc agccccagaa gccgcagatt attatgtttg aacggcatca
    85141 ccgccatcat cgcctgatcc agcgtgccga cgccaatttc agccagcaac gccttcttgt
    85201 tactgtcggc aaaccaggcc gcacatccct gactgaacgt ttgttcatcc ggttcttctg
    85261 acccgactaa atcaccagac cagagtgatt cattaaagcg gtccattaat gtgcgggcac
    85321 tatgtgccag caccaggctg gggcgggatt ctggcgaata gaaagcaagc caggttttga
    85381 ccagccgatc gtacatggca ttggccgttg ccattgttgg cagaccaaaa aacagcccct
    85441 gagctttccc cgcagctatc aacctgtgcg ccaggataag cgccgcttct gttttacctg
    85501 cgccagtcac atcttccaga ataaataact gcggtccttg ctggctgata tccagatcca
    85561 gcaccttttg ctgtaacggt gtcggattct caataaaagg aaacagcgta ttaattccgt
    85621 tgaaaggcgc gacttttgct tttagaggaa agacggttaa ggcgttttga gcctgaatta
    85681 aagttttctg ccagtaacct ttaatatcca ttggatgtgc gacgcgtgga aaaaatcgcg
    85741 ttgacgaacc cgtccagtct gcgagcacga cggttgcaga gatataccag gaaaggtgtt
    85801 ttataagttc aatgccctca tcatcatccc agaatgcggg gatctctatg agcggaaaca
    85861 gtggtttgat ttcaaggaga aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat
    85921 ccaattcatc catacgatca gggggctgac catgatgccc tgtcgtcacg ggcatccaca
    85981 tttctatcac tcgcctaagt ttgcgcggag agagcgaaga tgaaggaagt aactcctgac
    86041 attcacttaa ataatgattc cacaaccagt agcccagcgt tgaatgagag attttttcgt
    86101 aattttttct ggcaccttcc tgaatcttga gttcaggggg caggtaaagt tgttgaaaag
    86161 agcgggcaaa ttttccaata tcgtgccagc acagcaacca ggcgaaaaat tgagccgcct
    86221 gttccttgtc agaaatccct aattgacgaa aataatcagc cagcccgaag caatttcttt
    86281 taaccattaa atagcccatc gcggctacat ccagcgaatg ccagcaaaga aggtgatagt
    86341 cgtcgccccc gtctttctcg ccgcgtcggg tttttcccca gaaatcaaaa aaagtcacaa
    86401 tatttttatc cttcagtaa
//
LOCUS       DAZFKM010000005        76378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFKM010000005 DAZFKM010000000
VERSION     DAZFKM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..548
                     /locus_tag="ACHKAY_000377"
     CDS             <1..548
                     /locus_tag="ACHKAY_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5699113.1"
                     /translation="RHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTV
                     TLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYS
                     VSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEI
                     YGMWIDDMNDEQIAMLNARLS"
     gene            600..1535
                     /gene="ttcA"
                     /locus_tag="ACHKAY_000378"
     CDS             600..1535
                     /gene="ttcA"
                     /locus_tag="ACHKAY_000378"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO5699114.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1664..3037)
                     /gene="dbpA"
                     /locus_tag="ACHKAY_000379"
     CDS             complement(1664..3037)
                     /gene="dbpA"
                     /locus_tag="ACHKAY_000379"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO5699115.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3067..3240)
                     /gene="ynaL"
                     /locus_tag="ACHKAY_000380"
     CDS             complement(3067..3240)
                     /gene="ynaL"
                     /locus_tag="ACHKAY_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO5699116.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3515..4498)
                     /gene="zntB"
                     /locus_tag="ACHKAY_000381"
     CDS             complement(3515..4498)
                     /gene="zntB"
                     /locus_tag="ACHKAY_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO5699117.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4512..4559)
                     /gene="ydaN"
                     /locus_tag="ACHKAY_000382"
     CDS             complement(4512..4559)
                     /gene="ydaN"
                     /locus_tag="ACHKAY_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO5699118.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4753..5985
                     /gene="dgcM"
                     /locus_tag="ACHKAY_000383"
     CDS             4753..5985
                     /gene="dgcM"
                     /locus_tag="ACHKAY_000383"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO5699119.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6006..6569)
                     /gene="smrA"
                     /locus_tag="ACHKAY_000384"
     CDS             complement(6006..6569)
                     /gene="smrA"
                     /locus_tag="ACHKAY_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO5699120.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6899..7807)
                     /gene="abgR"
                     /locus_tag="ACHKAY_000385"
     CDS             complement(6899..7807)
                     /gene="abgR"
                     /locus_tag="ACHKAY_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5699121.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7983..9293
                     /gene="abgA"
                     /locus_tag="ACHKAY_000386"
     CDS             7983..9293
                     /gene="abgA"
                     /locus_tag="ACHKAY_000386"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO5699122.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9293..10738
                     /gene="abgB"
                     /locus_tag="ACHKAY_000387"
     CDS             9293..10738
                     /gene="abgB"
                     /locus_tag="ACHKAY_000387"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO5699123.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10775..12301
                     /gene="abgT"
                     /locus_tag="ACHKAY_000388"
     CDS             10775..12301
                     /gene="abgT"
                     /locus_tag="ACHKAY_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO5699124.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            12312..12827
                     /gene="ogt"
                     /locus_tag="ACHKAY_000389"
     CDS             12312..12827
                     /gene="ogt"
                     /locus_tag="ACHKAY_000389"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO5699125.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13022..13774
                     /gene="fnr"
                     /locus_tag="ACHKAY_000390"
     CDS             13022..13774
                     /gene="fnr"
                     /locus_tag="ACHKAY_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO5699126.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            13926..14876
                     /gene="uspE"
                     /locus_tag="ACHKAY_000391"
     CDS             13926..14876
                     /gene="uspE"
                     /locus_tag="ACHKAY_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO5699127.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(14926..15183)
                     /gene="ynaJ"
                     /locus_tag="ACHKAY_000392"
     CDS             complement(14926..15183)
                     /gene="ynaJ"
                     /locus_tag="ACHKAY_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO5699128.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            15427..16458
                     /gene="ynaI"
                     /locus_tag="ACHKAY_000393"
     CDS             15427..16458
                     /gene="ynaI"
                     /locus_tag="ACHKAY_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO5699129.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(16509..18122)
                     /gene="mppA"
                     /locus_tag="ACHKAY_000394"
     CDS             complement(16509..18122)
                     /gene="mppA"
                     /locus_tag="ACHKAY_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO5699130.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(18459..19358)
                     /gene="pgrR"
                     /locus_tag="ACHKAY_000395"
     CDS             complement(18459..19358)
                     /gene="pgrR"
                     /locus_tag="ACHKAY_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO5699131.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            19496..20416
                     /locus_tag="ACHKAY_000396"
     CDS             19496..20416
                     /locus_tag="ACHKAY_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5699132.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            20416..21348
                     /locus_tag="ACHKAY_000397"
     CDS             20416..21348
                     /locus_tag="ACHKAY_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO5699133.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            21366..22004
                     /locus_tag="ACHKAY_000398"
     CDS             21366..22004
                     /locus_tag="ACHKAY_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5699134.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            22313..23041
                     /gene="mpaA"
                     /locus_tag="ACHKAY_000399"
     CDS             22313..23041
                     /gene="mpaA"
                     /locus_tag="ACHKAY_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO5699135.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(23016..23981)
                     /gene="ycjG"
                     /locus_tag="ACHKAY_000400"
     CDS             complement(23016..23981)
                     /gene="ycjG"
                     /locus_tag="ACHKAY_000400"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO5699136.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            24100..24606
                     /gene="tpx"
                     /locus_tag="ACHKAY_000401"
     CDS             24100..24606
                     /gene="tpx"
                     /locus_tag="ACHKAY_000401"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO5699137.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(24650..26191)
                     /gene="tyrR"
                     /locus_tag="ACHKAY_000402"
     CDS             complement(24650..26191)
                     /gene="tyrR"
                     /locus_tag="ACHKAY_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO5699138.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(26339..27400)
                     /gene="ycjF"
                     /locus_tag="ACHKAY_000403"
     CDS             complement(26339..27400)
                     /gene="ycjF"
                     /locus_tag="ACHKAY_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO5699139.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(27397..28794)
                     /gene="ycjX"
                     /locus_tag="ACHKAY_000404"
     CDS             complement(27397..28794)
                     /gene="ycjX"
                     /locus_tag="ACHKAY_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO5699140.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            28949..29947
                     /gene="ycjW"
                     /locus_tag="ACHKAY_000405"
     CDS             28949..29947
                     /gene="ycjW"
                     /locus_tag="ACHKAY_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5699141.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(30058..30963)
                     /gene="ompG"
                     /locus_tag="ACHKAY_000406"
     CDS             complement(30058..30963)
                     /gene="ompG"
                     /locus_tag="ACHKAY_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO5699142.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(31008..32090)
                     /locus_tag="ACHKAY_000407"
     CDS             complement(31008..32090)
                     /locus_tag="ACHKAY_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5699143.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(32104..32763)
                     /gene="pgmB"
                     /locus_tag="ACHKAY_000408"
     CDS             complement(32104..32763)
                     /gene="pgmB"
                     /locus_tag="ACHKAY_000408"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO5699144.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(32760..35027)
                     /gene="ycjT"
                     /locus_tag="ACHKAY_000409"
     CDS             complement(32760..35027)
                     /gene="ycjT"
                     /locus_tag="ACHKAY_000409"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO5699145.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(35024..36079)
                     /gene="ycjS"
                     /locus_tag="ACHKAY_000410"
     CDS             complement(35024..36079)
                     /gene="ycjS"
                     /locus_tag="ACHKAY_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO5699146.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(36089..36877)
                     /gene="ycjR"
                     /locus_tag="ACHKAY_000411"
     CDS             complement(36089..36877)
                     /gene="ycjR"
                     /locus_tag="ACHKAY_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO5699147.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(36895..37947)
                     /gene="ycjQ"
                     /locus_tag="ACHKAY_000412"
     CDS             complement(36895..37947)
                     /gene="ycjQ"
                     /locus_tag="ACHKAY_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO5699148.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(37978..38820)
                     /gene="ycjP"
                     /locus_tag="ACHKAY_000413"
     CDS             complement(37978..38820)
                     /gene="ycjP"
                     /locus_tag="ACHKAY_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5699149.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(38807..39688)
                     /gene="ycjO"
                     /locus_tag="ACHKAY_000414"
     CDS             complement(38807..39688)
                     /gene="ycjO"
                     /locus_tag="ACHKAY_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5699150.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(39709..41001)
                     /gene="ycjN"
                     /locus_tag="ACHKAY_000415"
     CDS             complement(39709..41001)
                     /gene="ycjN"
                     /locus_tag="ACHKAY_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5699151.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(41015..42694)
                     /gene="ycjM"
                     /locus_tag="ACHKAY_000416"
                     /pseudo
     CDS             complement(41015..42694)
                     /gene="ycjM"
                     /locus_tag="ACHKAY_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(42906..43220)
                     /gene="pspE"
                     /locus_tag="ACHKAY_000417"
     CDS             complement(42906..43220)
                     /gene="pspE"
                     /locus_tag="ACHKAY_000417"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO5699152.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(43295..43516)
                     /gene="pspD"
                     /locus_tag="ACHKAY_000418"
     CDS             complement(43295..43516)
                     /gene="pspD"
                     /locus_tag="ACHKAY_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO5699153.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(43525..43884)
                     /gene="pspC"
                     /locus_tag="ACHKAY_000419"
     CDS             complement(43525..43884)
                     /gene="pspC"
                     /locus_tag="ACHKAY_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO5699154.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(43884..44108)
                     /gene="pspB"
                     /locus_tag="ACHKAY_000420"
     CDS             complement(43884..44108)
                     /gene="pspB"
                     /locus_tag="ACHKAY_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO5699155.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(44162..44830)
                     /gene="pspA"
                     /locus_tag="ACHKAY_000421"
     CDS             complement(44162..44830)
                     /gene="pspA"
                     /locus_tag="ACHKAY_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO5699156.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            44997..45974
                     /gene="pspF"
                     /locus_tag="ACHKAY_000422"
     CDS             44997..45974
                     /gene="pspF"
                     /locus_tag="ACHKAY_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO5699157.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(46094..47358)
                     /gene="puuE"
                     /locus_tag="ACHKAY_000423"
                     /pseudo
     CDS             complement(46094..47358)
                     /gene="puuE"
                     /locus_tag="ACHKAY_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(47396..48676)
                     /gene="puuB"
                     /locus_tag="ACHKAY_000424"
     CDS             complement(47396..48676)
                     /gene="puuB"
                     /locus_tag="ACHKAY_000424"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO5699158.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(48678..50165)
                     /gene="puuC"
                     /locus_tag="ACHKAY_000425"
     CDS             complement(48678..50165)
                     /gene="puuC"
                     /locus_tag="ACHKAY_000425"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO5699159.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(50440..50997)
                     /gene="puuR"
                     /locus_tag="ACHKAY_000426"
     CDS             complement(50440..50997)
                     /gene="puuR"
                     /locus_tag="ACHKAY_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO5699160.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(51024..51788)
                     /gene="puuD"
                     /locus_tag="ACHKAY_000427"
     CDS             complement(51024..51788)
                     /gene="puuD"
                     /locus_tag="ACHKAY_000427"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO5699161.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            52000..53418
                     /gene="puuA"
                     /locus_tag="ACHKAY_000428"
     CDS             52000..53418
                     /gene="puuA"
                     /locus_tag="ACHKAY_000428"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO5699162.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            53567..53731
                     /locus_tag="ACHKAY_000429"
     CDS             53567..53731
                     /locus_tag="ACHKAY_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699163.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            53721..55106
                     /gene="puuP"
                     /locus_tag="ACHKAY_000430"
     CDS             53721..55106
                     /gene="puuP"
                     /locus_tag="ACHKAY_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO5699164.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            55240..55485
                     /gene="ymjA"
                     /locus_tag="ACHKAY_000431"
     CDS             55240..55485
                     /gene="ymjA"
                     /locus_tag="ACHKAY_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO5699165.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            55798..57441
                     /gene="sapA"
                     /locus_tag="ACHKAY_000432"
     CDS             55798..57441
                     /gene="sapA"
                     /locus_tag="ACHKAY_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO5699166.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            57438..58403
                     /gene="sapB"
                     /locus_tag="ACHKAY_000433"
     CDS             57438..58403
                     /gene="sapB"
                     /locus_tag="ACHKAY_000433"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO5699167.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            58390..59280
                     /gene="sapC"
                     /locus_tag="ACHKAY_000434"
     CDS             58390..59280
                     /gene="sapC"
                     /locus_tag="ACHKAY_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO5699168.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            59280..60272
                     /gene="sapD"
                     /locus_tag="ACHKAY_000435"
     CDS             59280..60272
                     /gene="sapD"
                     /locus_tag="ACHKAY_000435"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO5699169.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            60274..61080
                     /gene="sapF"
                     /locus_tag="ACHKAY_000436"
     CDS             60274..61080
                     /gene="sapF"
                     /locus_tag="ACHKAY_000436"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO5699170.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            61148..61501
                     /gene="ycjD"
                     /locus_tag="ACHKAY_000437"
     CDS             61148..61501
                     /gene="ycjD"
                     /locus_tag="ACHKAY_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO5699171.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            61869..62657
                     /gene="fabI"
                     /locus_tag="ACHKAY_000438"
     CDS             61869..62657
                     /gene="fabI"
                     /locus_tag="ACHKAY_000438"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO5699172.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            62802..63931
                     /gene="yciW"
                     /locus_tag="ACHKAY_000439"
                     /pseudo
     CDS             62802..63931
                     /gene="yciW"
                     /locus_tag="ACHKAY_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            63999..65933
                     /gene="rnb"
                     /locus_tag="ACHKAY_000440"
     CDS             63999..65933
                     /gene="rnb"
                     /locus_tag="ACHKAY_000440"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO5699173.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPSLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(66011..66115)
                     /locus_tag="ACHKAY_000441"
     CDS             complement(66011..66115)
                     /locus_tag="ACHKAY_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699174.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            66169..68154
                     /gene="pdeR"
                     /locus_tag="ACHKAY_000442"
     CDS             66169..68154
                     /gene="pdeR"
                     /locus_tag="ACHKAY_000442"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5699175.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(68136..68264)
                     /gene="ymiD"
                     /locus_tag="ACHKAY_000443"
     CDS             complement(68136..68264)
                     /gene="ymiD"
                     /locus_tag="ACHKAY_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO5699176.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            68302..68475
                     /gene="yciZ"
                     /locus_tag="ACHKAY_000444"
     CDS             68302..68475
                     /gene="yciZ"
                     /locus_tag="ACHKAY_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO5699177.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            68565..69314
                     /gene="yciT"
                     /locus_tag="ACHKAY_000445"
     CDS             68565..69314
                     /gene="yciT"
                     /locus_tag="ACHKAY_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO5699178.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            69583..69801
                     /gene="osmB"
                     /locus_tag="ACHKAY_000446"
     CDS             69583..69801
                     /gene="osmB"
                     /locus_tag="ACHKAY_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO5699179.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(69927..70253)
                     /gene="yciH"
                     /locus_tag="ACHKAY_000447"
     CDS             complement(69927..70253)
                     /gene="yciH"
                     /locus_tag="ACHKAY_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO5699180.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(70253..70990)
                     /gene="pyrF"
                     /locus_tag="ACHKAY_000448"
     CDS             complement(70253..70990)
                     /gene="pyrF"
                     /locus_tag="ACHKAY_000448"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO5699181.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(71182..72351)
                     /gene="lapB"
                     /locus_tag="ACHKAY_000449"
     CDS             complement(71182..72351)
                     /gene="lapB"
                     /locus_tag="ACHKAY_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO5699182.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(72358..72666)
                     /gene="lapA"
                     /locus_tag="ACHKAY_000450"
     CDS             complement(72358..72666)
                     /gene="lapA"
                     /locus_tag="ACHKAY_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO5699183.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(72815..73579)
                     /gene="pgpB"
                     /locus_tag="ACHKAY_000451"
     CDS             complement(72815..73579)
                     /gene="pgpB"
                     /locus_tag="ACHKAY_000451"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO5699184.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            73749..74339
                     /gene="ribA"
                     /locus_tag="ACHKAY_000452"
     CDS             73749..74339
                     /gene="ribA"
                     /locus_tag="ACHKAY_000452"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO5699185.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(74403..>76378)
                     /gene="acnA"
                     /locus_tag="ACHKAY_000453"
     CDS             complement(74403..>76378)
                     /gene="acnA"
                     /locus_tag="ACHKAY_000453"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO5699186.1"
                     /translation="AAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKH
                     GVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQ
                     VELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFA
                     ASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAK
                     KAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGP
                     LPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINL
                     AAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVT
                     RSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIK
                     PDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHL
                     PDSDVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFER
                     IHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQ
                     EVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        18616 a        19875 c        18879 g        19008 t
ORIGIN      
        1 ttcgtcacgg ggaactggca gctctggcgt gggaggatgt ggatctcgaa aagggaatag
       61 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
      121 ggatccggac agtaacacta ctgcagcctg ctcttgaagc actgaaggag caatacaaac
      181 tgaccgggca tcatcgcaaa agcgaaatca ccttttatca tcgggagtac ggcagaaccg
      241 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacggaaaa caaaaacctt
      301 attactcggt aagcagtttg ggggcaaggt ggaatgcagc agtaaaacgt gctggtattc
      361 gccgccgtaa tccgtaccat acgcggcata cttttgcctg ctggctgttg acggcaggag
      421 cgaacccggc atttatagcc agccaaatgg ggcatgaaac tgcgcagatg gtgtatgaaa
      481 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      541 tatcatagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      601 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      661 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      721 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      781 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      841 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      901 agattgttga agagaatact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      961 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
     1021 gggcgacgaa gatcgcgttg ggtcaccatc gtgacgatat cctgcaaacg ttgttcttaa
     1081 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1141 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1201 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1261 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1321 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1381 attttaaagg cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1441 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1501 atgagttacg actgaatgtg gttgaagtga aataaccagg atagcgcccg atgcgcaagc
     1561 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1621 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1681 cccggcacgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1741 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1801 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1861 cttttttccc gccatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1921 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1981 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     2041 ctgtacgacc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2101 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtagca accagtacac
     2161 gggcgctacc gttagcaaaa cgtaccagag tctgatcgcg atcgcgttgc tccagatcgc
     2221 cgtgtaacga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2281 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2341 gcaacagagg gattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2401 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgtactcgt ccgctgattg
     2461 cggcgatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2521 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2581 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2641 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2701 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2761 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2821 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2881 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2941 cggcggcctg caccggcgtc atggttaaat aacccaactc attaaggttc gtgagttggg
     3001 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3061 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3121 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3181 aacaggatcg ctaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3241 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3301 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3361 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3421 atggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3481 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3541 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3601 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taaccctgtc agaaaggtac
     3661 tgggtaaaaa gaccattgcc atcaatgaca ttgtataggt acgacgagct aaattttcct
     3721 gcatcacctg agcgatttca tccgccatca caccagtccg tgctatacag gcgtcgattt
     3781 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3841 tcatccacgg caaacgttca ctggcaagac gagcatagac atcacgttgc ggtgccatat
     3901 agcgacgcat cacgattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3961 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     4021 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4081 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4141 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4201 gttcatcggt gctgccgttt atacagcgca atgtaataag cgtgccttca ccgagacggc
     4261 tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4321 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4381 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4441 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4501 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4561 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4621 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4681 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4741 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4801 tggattgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4861 atgcaagacc tcacgtttag tcagctacga accggaccct attccgtctc ctcacaaaaa
     4921 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4981 gttcagcgta aagaagagga aacagcatta agctgtcggc ttgttttgcg agagctgaca
     5041 gaaacagaac cggtgattat tttcgaaggt atcgaagcgc cggcaacgct gggtttaaaa
     5101 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5161 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5221 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5281 tgggaaatta atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5341 cccggcggtc ataaaccttt gaattttgtt cataaactgg cggatggatc tactcgtcat
     5401 gtgcagacct atgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgtg
     5461 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc tcaccatgac
     5521 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5581 cagcacctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5641 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5701 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5761 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgt
     5821 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgtt ttaccgtcag cattggtgtg
     5881 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5941 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     6001 cgagatcaac ggctgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6061 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc ggtgcaatat
     6121 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6181 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6241 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6301 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6361 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6421 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6481 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6541 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6601 tgataaccag accgggtcgg tccaacaacg tcttacccaa aattccagta ataagttcca
     6661 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6721 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6781 ccgttataac acagtcaccg gcgcagagga gacaatgccg gacttaagac gcggatgcac
     6841 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6901 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6961 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     7021 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7081 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7141 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7201 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7261 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7321 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7381 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7441 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7501 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atttcctctt
     7561 gcgctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7621 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7681 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7741 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7801 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7861 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7921 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7981 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     8041 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8101 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8161 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8221 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcacg ggtatcgtcg
     8281 ccaccctgga taccggtcgc cccggtccgg ttatggcttt ccgtgtcgat atggacgcgc
     8341 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8401 gtaacgccgg aatgatgcat gcctgtggtc atgatgggca taccgccatt gggcttgggc
     8461 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8521 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8581 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8641 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc gggaccgccg
     8701 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcaa
     8761 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8821 ttatgcaggc aggaagcggc cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8881 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8941 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg cctgatgggt gcagctaccg
     9001 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9061 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9121 tgatggcccg cgtgcagcac catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9181 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9241 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9301 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9361 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9421 ggcttcggcg ctggaatctg caagcttcac cgttacccgc aacgtaggca atatcccaaa
     9481 tgcatttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9541 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9601 tgaaaatggt cacggttgcg gacacaattt gctgggaacc gccgcctttg ccgctgcaat
     9661 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9721 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9781 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggcatgttca atacccgcac
     9841 gctggcaaac attcaggcat catggtgctt taaagggatc gcagcacatg ccgcgaattc
     9901 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9961 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
    10021 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10081 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10141 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10201 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10261 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10321 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10381 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10441 tgcggcatcg actgatgtcg gcgacgtcag ttggaaactg cctgttgccc agtgtttcag
    10501 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10561 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10621 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagttacgga
    10681 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10741 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10801 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttccccatcc
    10861 ttttttgctc tttatctatt tgataatcgt actcatggtg acgacggcaa ttttgtcagc
    10921 ctttggcgtc agtgcgaaaa acccgaccga tggtacgccg gtggtggtga aaaacctgct
    10981 cagtgtggaa ggattacact ggtttttacc caatgtgatt aaaaacttta gcggttttgc
    11041 tccacttgga gcgatcctgg cgctggtttt aggtgccggc ctagcggagc gcgtcggctt
    11101 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11161 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcggcgttag tgatcatgcc
    11221 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11281 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11341 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11401 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11461 cttgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11521 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11581 gctacttttt attgctgcga tcgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11641 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactaatcat
    11701 tctttttttc tttgtggttt cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11761 ggcggattta ccgcatttaa tgattgaacc gatgaaagag atggcgggat ttatcgtgat
    11821 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg ggaaattcat
    11881 cgccgtgggg ctgaccgata tacttgaaag ttcagggctt agcggcatcc cggcgtttgt
    11941 cggtctggcg ttgctttcct ctttcttatg catgtttatt gccagcggtt ccgcaatctg
    12001 gtcgattctg gcccccattt tcgtaccaat gtttatgcta cttggctttc acccggcatt
    12061 tgcgcaaatc ctctttcgta ttgccgactc atccgtattg cctttagcgc cggtatctcc
    12121 ttttgttcca ctgtttcttg gattcctgca acgctacaaa ccagacgcga aactgggtac
    12181 ttactattcg ttagtcttgc cctatccgct tatctttttg gtggtatggc tgctgatgtt
    12241 gctggcgtgg tatcttgttg gtctgccgat aggtccgggg atttacccac gtttgtctta
    12301 agagagaacg gatgctgaga ttacttgaag aaaaaattgc cacgccactg ggtccactgt
    12361 gggtgatgtg cgatgagcaa tttcgcctgc gggcggttga atgggaagag tacagcgaac
    12421 gcatggtgca gctgctggac atccattatc gtaaagaagg ctatgagcgc atttctgcca
    12481 ccaacccagg cggtttaagc gacaagcttc gtgattattt tgccggtaat cttagcatta
    12541 ttgatacgct tcccactgcc acagggggga cgccatttca gcgcgaagtc tggaaaactc
    12601 tacgcactat cccctgcggg caggtaatgc attacggcca actggctgaa caattgggcc
    12661 gccctggcgc ggcgcgtgcc gtaggtgcgg caaatggatc gaatcccatc agcatcgtcg
    12721 taccttgcca tcgggttatt ggtcgaaacg gcaccatgac cggatatgca ggcggagttc
    12781 agcgaaaaga gtggttattg cgccacgaag gttatctttt gctgtaaaca ttaaacaatt
    12841 tgtgccagct tgttcacact tttatgtaaa gttaccctta acaacttaag ggttttcaaa
    12901 tagatagaca tatatttaca tctaatatcg gaattctctg ctgttaaggt ttgcttagac
    12961 ttacttgctc cctaaaaaga tgttaaaatt gacaaatatc aattacggct tgagcagacc
    13021 tatgatcccg gaaaagcgaa ttatacggcg cattcagtct ggcggttgtg ctatccattg
    13081 ccaggattgc agcatcagcc agctttgcat cccgttcaca ctcaacgaac atgagcttga
    13141 tcagcttgat aatatcattg agcggaagaa gcctattcag aaaggccaga cgctgtttaa
    13201 ggctggtgat gaacttaaat cgctttatgc catccgctcc ggtacgatta aaagttatac
    13261 catcactgag caaggcgacg agcaaatcac tggtttccat ttagctggcg acctggtggg
    13321 atttgacgcc atcggcagcg gccatcaccc gagcttcgcg caggcgctgg aaacctcgat
    13381 ggtatgtgaa atcccgttcg aaacgctgga cgatttgtcc ggtaaaatgc cgaatctgcg
    13441 tcagcagatg atgcgtctga tgagcggtga aatcaaaggc gatcaggaca tgatcttgct
    13501 gttgtcgaag aaaaatgccg aggaacgtct ggctgcattc atctacaacc tgtcccgtcg
    13561 ttttgcccaa cgcggcttct cccctcgtga attccgcctg acgatgactc gtggcgatat
    13621 cggtaactat ctgggcctga cggtagaaac catcagccgt ctgctgggtc gcttccagaa
    13681 aagcggtatg ctggcagtca aaggtaaata catcaccatc gaaaataacg atgcgctggc
    13741 ccagcttgct ggtcatacgc gtaacgttgc ctgatttttc cgcataactc actatccttc
    13801 tgtcatatca ttaaattttt ctgatttatt gatctggcag aaggttcatc actgtttcat
    13861 tcaccagata tgggttaatc ttttaattac aaactgcgtt gacagttgtt gtaaggagac
    13921 cctgtatggc tatgtatcag aacatgctcg ttgttatcga tcctaaccag gacgaccaac
    13981 cagcattgcg gcgagctgtt tatttacatc aacggattgg tggcaaaatt aaagcctttt
    14041 tgccgatcta tgacttctca tacgaaatga ccaccctgct ctccccggac gaacgtaccg
    14101 ctatgcgtca gggcgtcatc agccagcgta cagcctggat ccacgagcag gcaaaatatt
    14161 atctcaatgc tggcgttccc attgaaatta aagtggtctg gcataaccgt cccttcgaag
    14221 ccatcattca ggaagtgatc agcggcgggc acgatttggt gctaaaaatg gcgcaccaac
    14281 atgaccgtct ggaagcggtg atttttacgc caacggactg gcatctgtta cgcaaatgcc
    14341 caagcccggt gtggatggtg aaagaccagc cgtggccgga aggaggcaag gcgctggtgg
    14401 cggtgaatct cgccagtgaa gagccgtatc ataatgcgct caatgaaaaa ctggtcaaag
    14461 agacgatcga actggcagaa caagtcaacc ataccgaagt tcatctggtt ggcgcttatc
    14521 cggtaacgcc aattaatatc gcgattgaac tgccggaatt tgatccgagc gtttataacg
    14581 atgccattcg tgggcaacat ttgctggcaa tgaaagctct gcggcagaaa ttcggcatta
    14641 atgaaaacat gacgcacgta gaaaaaggtc tgccagaaga ggtgattcct gatttggcgg
    14701 agcacttaca ggcggggatt gtggttctgg gcacggtagg acgcaccggt atttcagcag
    14761 cattcctcgg caacacggcg gaacaggtga ttgatcatct tcgctgcgat ctgttggtca
    14821 ttaaacctga ccagtatcag acacccgttg aactggatga cgaagaagac gattaacgac
    14881 tcttcaataa ctgacaaggc ccgagttatc gggcctattt tttacttact gctcttcgcc
    14941 gccaaggaaa taaattccca acgggattgc cagcaacaca gtaaagacca ggctataaac
    15001 aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc cactcagaca gcggaatatt
    15061 gtactgttct ataacgccgc cgatagtcgc gcgagtcaca accgacgccg caataatgaa
    15121 aaccgccaac aaaccaaaga gaaatttctt ccctttcgct gacttcagtt tcgccataat
    15181 catgatgtaa tccttgtgca gacaaatttc ttgttaccta aacgtaacaa taccatgaca
    15241 tttcaccgtt gtctgctctg aaaacctcac ctcttagctg gatttacaat caacgcattg
    15301 attagttagc taatgttgct acttccttcc ctgactattg atgatctgaa tgaattgtcg
    15361 cggtaaaata gccccgccag gggtttccgg catgacgaca attatctgga ggagcctgat
    15421 aaaacaatga tcgctgaact gtttacaaat aatgcgctta atctggtcat tattttcggt
    15481 agctgcgcag cattgattct gatgagcttt tggtttcgcc gcggaaatcg taaaagaaaa
    15541 ggttttttat tccatgcggt gcaattttta atctacacca taattatcag tgctgttggt
    15601 agcattatta attatgttat agaaaactat aaactcaaat ttatcacccc aggcgttatc
    15661 gattttatct gtacgtccct gattgcggtt attctgacga ttaagctatt tttgctgatt
    15721 aatcagtttg aaaaacagca gattaaaaaa ggtcgcgata tcaccagtgc gcggattatg
    15781 tcgcgcatta tcaaaatcac cattattgtg gtgcttgttc tgctttatgg cgaacatttc
    15841 ggcatgagcc tttctggctt gctgaccttt ggtggtattg gtggtctggc tgtcggtatg
    15901 gccggtaaag atattctgag taatttcttt tccgggatca tgctctattt cgaccgtcct
    15961 ttcagtattg gcgactggat ccgttcaccg gacagaaata tcgaaggtac agtagcggaa
    16021 attggctggc gaattaccaa aattacgacc tttgataatc gtccattgta cgtaccgaac
    16081 tcgctgtttt cgtcgatcag cgtagaaaac ccaggacgaa tgaccaaccg ccgcattacc
    16141 acgaccattg gtttacgtta tgaggatgcg gcaaaagtgg gcgttattgt cgaagccgta
    16201 cgtgagatgc tgaaaaatca cccggccatc gaccagcgac aaaccttact ggtttatttc
    16261 aaccagtttg ccgactcgtc attgaatatt atggtttatt gctttaccaa aaccacggta
    16321 tgggctgaat ggcttgctgc acagcaagac gtttatttga agattatcga tattgtgcag
    16381 tcacacggcg cggactttgc cttccccagc cagacgctgt atatggataa cattacaccg
    16441 ccggaacagg ggcgctaaat cagacatcac tggcgttatc cacaagggta acgccagttc
    16501 ccatcacatc aatgcttcac aatatacata gtccggctgt acgcaacatc ttccgggtta
    16561 ttaatgggat aacctttcag ccacggcttg attaatcgtc cattggtata ttgataaatt
    16621 ggtgcaatcg gtgcttgctc catgaggatt ttttctgccg cgttgtaatc ggcattacgc
    16681 gctttaacgg tattttccat cgatgcctgg gccagaactt tgtcgtatgc cggattgtta
    16741 aagcgtgaaa tatttcctga atgcgttgac gttaataatg tcaggaaagt ggagggttca
    16801 ttataatccc ccacccatga ggcgcggata acatcaaaat tgccggtgtt acggctatcg
    16861 atataggttt tccattcctg attttgcaat ttaacatcta cgccaaggtt ctttttccac
    16921 atcgatgcta cagcaatcgc aattttttga tggttttctg aagtgttata caaaagcgtc
    16981 agcttcagcg gtttttgcgg accataacca gctgcgctca acaaagtttt tgcctgcgca
    17041 ttcagttctt cctgactcat ttgttcaaac ggcgaaggtt ccggcgtaaa tccggcggta
    17101 acatctggtg taaaatgcca cgctggcttt tcgcccgttc ccaacacttt ttcggtcatc
    17161 aggcggcgat ctatcgtcat acttaatgcc aggcgaacgc gctgatctgc cgtcggccct
    17221 ttttgcgtgt taaacgcata ataataggtc ccgagctgtg gcggcgtata aacctgcccc
    17281 ggaatatcct tcaacagctt ctgatacata tttttcggga aggattcggt gatatcaata
    17341 tcccccgcga ggtaacgctt agtggctgcg gattcctgat taattggcag gaaggtcact
    17401 ttttgcagta ccgttttggc gttatcccaa taatgggtat tcggtaccac gaccagtttt
    17461 tcattgacta cgcgatcttt aagaacataa gcgccattgc cgatcagatt tccgggtttc
    17521 gtccactctt taccgctttc tacgttggct ttttgcaccg ggaagaaggc aaagttagcg
    17581 gttaaattca caaaccacgg caacggttta tcaagctgaa ttttcaaagt atgggcatca
    17641 actgcggtga cgccaagctg gtctggcgta gctttaccat caataatcgc ctgtgcgttg
    17701 ttgattcccg ccagcgcggc aaaccatgca aatggcgata gcgttttcgg gtccaccaga
    17761 cgttgccagc tgtagacaaa atcttgcgcc gttaccggtg tgccatccgc ccattttgcg
    17821 ttatcgcgca gggtaaaagt ccagatacgg ttgtcattac ttttccactg agtcgcaacg
    17881 ccggggacaa tctccccttt ctcgttctga ttcaccagtc cttcaaacag atcgcgaatg
    17941 acctgaatct ctggcaggcc cacggcttta gcgggatcca gcgacgcagg ctcatcttta
    18001 atgtggcgca ccagctcctg cttctctgcc agtactgtgc cgctcggaac ttctgcagca
    18061 tacgaaagag aaatgctgct gaccaacagc gcacaacacg tgactgaaac agagtgcttc
    18121 ataagtttcc ctctaaccga cattaaaatt gatgcgtaat tatttgtttc gccttaaaga
    18181 aatgcaaaca acttccatca aaaaagtgac acaggaagca taaaatgttt tatgtatcaa
    18241 tatattaaat cagatttagt gtaaataatc gacgccaaaa tgcggtaaag cacatagaac
    18301 accgaacact acgttcaggc ttcgcacgtt caaaccagtt ttcggtatca taatcagcat
    18361 ctgtcgccat tgtacgaaga ataaaaaata accaaaacgt tgaaaggaac tgagaatacg
    18421 ttaagagaga aattaaatta atgtatcagc aagtagtgct aaacagcacc tttatacttc
    18481 agcctatcta taaataatga gaatgccgag ccagcatgtc gacggtgtgg atagtacagg
    18541 tgatgaccgg gtaaatctgg tgtgaattta tccaaaacac gtatcagttt tttttcttta
    18601 atagcccgct caacctgatc ataaggtaga tacgccaatc catgcccatc aattgcagca
    18661 tcaatgatca ggtctatcgt attcagtaaa agctgacctt ccatgcgaac acgaacttca
    18721 cgtccaccgc gtattaatct ccagcgattt gctgtacccg atgtgggaag atacaaatta
    18781 attgcctgat gatctattaa ttgtgacact gacgttggaa cacttcggcg agaaaaataa
    18841 tccggtgagc caacaatagc cattggtata tctggcccga ttcgaatggc gatcatatct
    18901 ttatccatct ccccacccag acggacgcct gcatcaaaac gttcagaaac gacatcggtc
    18961 aaaccataat caatggtgag ctgaatatca atttcaggat gcgatttcag gaatgtgcgc
    19021 attgctggta acaatatcgt ttttgctgca tgttctacag tagtaatacg tattgtcccg
    19081 gatgggcggt tctgcagctc gctcagggat gccagggctg aatctatgtc atgcaacata
    19141 gggccaagaa cagacaaaag atgctcgccc gcttcagttg gaacaacgct gcgcgtggtt
    19201 cgcgtcagaa gccgcaatcc caatcgttct tctatacgac gcactatctg gcttaaagct
    19261 gactgcgcca tgctcaggcg ggctgctgca cgagtgaagc tacgctcctc tgcaacgacg
    19321 acaaacgcca tcagatcagc gatttcttca cgcttcattc agtatctcca tgcaatctat
    19381 taatcattta aatgataggt ctaatcatga ttttgggtct aatcaactta atgatcaata
    19441 tctatagtga tatcaccaac cgttaaaacg aaccgacaac tccatgagga acggaatgat
    19501 gaataataaa gtcagcttca ctaacagcaa taatccaacc atctctttgt ctgcagtgag
    19561 ctatttccct ccaaaatttg atgaaacccg tcagtatcag gcaattgtgg tatctcaccc
    19621 aggtgggggc gttaaagaac aaacggccgg aacctatgcc aaaaaactgg cggaaaaggg
    19681 atttgtcact attgcttatg acgcatctta tcaaggtgaa agtggcggcg agccgcgtca
    19741 gttagaaaac ccatacattc gtactgaaga catcagtgcc gtgattgatt acctgacaac
    19801 gctttcttat gtcgacaata cccgaattgg tgcgatggga atttgtgccg gtgcaggata
    19861 tacagccaat gctgcaattc aggatcgccg catcaaggcc atcggtaccg ttagtgcggt
    19921 caatattggt tcgatattcc gtaatggttg ggaaaacaat gtgaaatcta ttgatgccct
    19981 gccatacgtt gaagcgggtt caaacgccag aaccagcgat attagcagcg gagaatacgc
    20041 cattatgcca ctggcaccaa tgaaggagtc cgatgcgccg aacgaagaac tgcgtcaggc
    20101 ctgggagtat taccacaccc ctcgcgcgca gtatccaaca gcaccgggtt acgctacttt
    20161 gcgcagcctt aaccagatta ttacctatga tgcttaccat atggcggaag tgtacctgac
    20221 tcagccgacg cagattgtgg cgggcagcca ggcagggagc aaatggatga gtgacgacct
    20281 gtacgatcga gcatcaagcc aggataaacg ctatcacatt gttgaaggcg caaaccatat
    20341 ggatctttat gacggcaaag cctatgtggc tgaagcaatt tcagtattag ctccgttctt
    20401 tgaggaaact ctgtaatgaa acatattcaa atccgcaata gtgatatggc ctggcatatt
    20461 gctgccaata ttcagtttcc accaaatttt gatgagtcaa aacagtatcc ggcgattatc
    20521 agcgtccatc cttttggcag ttgtaaggag cagacgtctg gcaatatcta tggcaaagca
    20581 ttggctgaaa aaggctatct cgttctcgct tatgacgcca gtttccaggg agaatctggt
    20641 ggggagcctc gctggataga agatccgaca cagcgcgtag aggatattag ccgtgttatc
    20701 gattatgcag taactctgcc atatgttaat gctgaacgta ttggcgtgct gggagtgtgt
    20761 gggggtggtg gctatgccat taatgccgcg ctgacagaaa aacgaattaa agcagtagtg
    20821 agtattacgg gtgttaatat cgggcgtctt tttcgggaag gtttcagcaa ttacgatccg
    20881 attggcgcat tgaatgcaat ggcgtcgcaa cgaaccaaag aggctcgtgg tggagaatta
    20941 cagatcaacg aattacttcc cgccagcctg gatgcagcaa aagcccatgg actgaccgaa
    21001 cgcgatgttt atgaagcaac tgactactat aaaacaccac gcgggcaaca accgggaggt
    21061 gcgacaaaaa tgttgttctc acatgcacag aaaacgctgg cctgggatgc cttcgcattt
    21121 actgaagtgt tactgactca gccggtgatg gtagtagttg gtgaaaaagt cggtgccttc
    21181 ggtgcttatc gtgatggact ggaagtctat ggccgggcga tggtttcgca ggatcgtcag
    21241 ttagtttcat tacccgattt ctcacattat gaactgtatg acaaaccgga agcagttcag
    21301 aaggcgctgg aaaaagttat tccttttttc aacacccatc tgggataagt ttcatgagaa
    21361 atattatgaa aaatgtactg attcttggtg ctggcggtca gatagcccgc catgtgatta
    21421 atcaactggc agataaacaa accattaaac aaactttatt tgcccgtcag ccagcaaaaa
    21481 tccacaaacc ctatccaact aatagcaaga ttattatggg agatgtactg aatcatgcag
    21541 cactgaagca ggccatgcag ggacaggacg ttgtgtatgc caaccttacg ggggaagatt
    21601 tagatattca ggcaaattca gtgatcgctg cgatgaaagc ctgtgatgtt aaacgtctga
    21661 tctttgttct ttccctcggt atttatgatg aagttccagg aaaatttggc gaatggaata
    21721 atgcagttat tggtgagccc ctaaaaccat tccgcagggc tgcggatgca attgaggctt
    21781 ctggactgga atatacaatt cttcgccctg cctggctgac tgatgaagat attattgatt
    21841 atgagcttac ctcacgcaat gaaccattca aaggtacgat cgtatcacgt aagagtgtcg
    21901 cagcattaat cacagatatc atcgataaac ctgagaaaca cattggtgag aatataggta
    21961 tcaatcaacc cgtgactgat ggtgacaagc cattattcat gtaaatcaat tacatagtta
    22021 ccatcgtaat gtactatgtg cagtacgatt tattaaactt attaaggtga actgatcaat
    22081 ccgatgcgat taacatgtgt agaaccagta agaataaaat acgccatgta tataacatta
    22141 cgacttacat ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac
    22201 ttccggcaaa aaagtgatac aagatcgatg acatagcgtt gaagaaaagg atttgataat
    22261 ccgcaaaatt tgcacaacac tgcctgtaat acttcctgaa ccagagacga tcatgaccgt
    22321 aaaccgccca cgcgccgaac gcggcgcatt tccgcccgga acagaacatt acggacgttc
    22381 attattgggc gcgccgttaa tctggtttcc ggccactgca gccagtcgcg aaagtggttt
    22441 gattctggct ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct cctgtgctct
    22501 gcggactttg acaccttctt tgcgccgcca tcatgtagtg ctgtgtgtga atcctgacgg
    22561 ctgccagctg gggttacggg ccaatgctaa tggtgtggat ttaaaccgaa actttccggc
    22621 ggcgaactgg aaggaaggtg aaacagttta tcgctggaac agcgccgctg aagaacgtga
    22681 tgtcgtattg ctgacaggtg acaaacctgg ctctgaacct gaaacccagg cgctgtgcca
    22741 gcttatacac cgcattcaac ccgcctgggt ggtctcattc cacgatccgc tggcctgtat
    22801 tgaagatccc agacacagcg aattaggcga atggctggcc caggcgtttg aattgcctct
    22861 ggtaaccagc gtcggttatg aaacacctgg ctctttcggt agttggtgtg cggatctcaa
    22921 cctgcattgc atcaccgcag agtttccgcc catctcctcc gacgaagcca gcgaaaaata
    22981 tctctttgcg atggcaaact tgctgcgctg gcatcctaaa gatgcaattc gcccgtcgtg
    23041 aactgaagcg ccggttccac atctaccgcc agccaggtcg gtccgtcaag atcgacgaaa
    23101 ctgacctgcg gcaccagcgg taaagcggcg ctaatggccc gagaggtaca caacatgcag
    23161 cccagcatca gacgaaaacc ttgtgcacgc gcttcagtcg ccagcgccag cgcttccgtc
    23221 agaccaccgg ttttatcaag cttaatgtta accatctcat agcgcccttt cagcgccttc
    23281 agattgctac gtgtatgaca actttcatct gcacaaatcg gtaacggatg aataaaattc
    23341 tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac gcctaaatcc
    23401 gccaatagct ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt tgcgtcaacg
    23461 atcagcgtcg catcgggcac agctgtgcga attgccacca tccgctcact gataagatgg
    23521 ttatccagct tcactttcag taatttcgcg cctgcctgcc agagtgttga tgcactattg
    23581 gccatctgat caggcgtacc gatgactacc gtctgtgcag tggtgactgt cccgggaagc
    23641 gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgcggccag atcccacaat
    23701 gcacaatcca gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa ctcctcccgt
    23761 gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac cgaggcatca
    23821 ctttccccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc ctcttcttcc
    23881 agttcaacca ccaccacgcg cgcttcactg cgacttcccc gggcaatcac aaacggggta
    23941 tgtaagggcc aggcttcctc aaatacctta acggttctca tctttactcc ttgttgtcgc
    24001 ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt agcataaact
    24061 aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac cgttcatttc
    24121 cagggcaacc cggttacagt cgccaattcc atcccgcagg cgggtagcaa agcgcagact
    24181 tttactctcg tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc gggtaaacgc
    24241 aaagtgctga acattttccc gagtattgat accggtgttt gcgccgcatc agtacgtaag
    24301 tttaaccaac tggcaactga gatcgacaac accgttgtgc tgtgtatctc tgccgatctg
    24361 ccgttcgccc agtctcgttt ctgcggcgca gaaggtctga acaacgttat caccctctcc
    24421 actttccgta acgctgaatt tctgcaagcc ttcggtgtgg caattgctga tggcccactg
    24481 aaaggtctgg cagcgcgtgc cgttgtggtt attgacgaaa atgacaatgt gattttcagc
    24541 cagctggtgg atgaaatcac caccgagccg gattacgaag cagctctggc tgtactgaaa
    24601 gcataagtta atatgcctga tggtgttgca ccatcaggca tattcgcgct tactcttcgt
    24661 tcttcttctg actcagacca tattcccgca acttattggc aatcgcggta tgtgaaacgc
    24721 cgagacgttt tgccagtttg cgcgtgctgg gataattgcg ataaagctgg gttaataccg
    24781 agcgttcaaa acggctggtg atttcgtcca gcgaaccttc catcgcatct tcgcccacgg
    24841 ctaccgttgc ggcgtcataa tccggcaaca aaatatcctg tggacgcagc tcataaccgt
    24901 ccagttgtgt cagtgcgcga tagatagcgt tctttaactg ccgcacattt cccggccacg
    24961 cataacgcgt aagtacagta ttcaggtcag cggccagttt cggacgcggc acgccctgct
    25021 cgtcggcaaa gcgggcgacg aacagctcag ttaacggcat gatgtcctgc ggacagtcac
    25081 gtagcggcgg cagattgagc gtcaacacgt tcagacgata atagagatct tcacggaaca
    25141 tgcctttttg caccagttcg accagattct tctgcgtagc gcaaatcacc cgcacatcga
    25201 catgcacctc atggtcttcg ccaacccgac ggaaagtgcc atcattaagg aaacgcagta
    25261 atttcgcctg catccgtggt gacatttccc ctatttcatc caacagcacc gaaccaccgt
    25321 tcgcctgctc aaagaatcct ttcttccctt ccggagcatg accaaacagt tcactctcga
    25381 ccgcatcttc cggtatagac gcacagttca gcgccaggta aggtttgccc gctctggggc
    25441 ttgcctgatg gcaggcgtag gcaaagagat ctttacctgt acctgtgtca cccgtaatca
    25501 gcagcggcgc gcttagcatc gccagtttct gcgcctgttc gacaacatgc ttcattttcg
    25561 ggctgacggc gacaatttga ctgaaggcgc tgacgtcctg ggcggcgaca ttttgcaact
    25621 ggcggcccat acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg acgtgttgat
    25681 cattttcatc ctgaagataa acaggcgtaa tctccatcag gaaattctgc ccattaataa
    25741 cgacatgctc gttatgggaa tcttgcggtt cgctttccag ccaacgtaaa aaattaaagc
    25801 cgttaatcaa ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc gcaaaaagct
    25861 gacagctcgc cgggttcgcc atatccactt tgcttttcat atcgacagag agcacaggtt
    25921 cgggcaacgc ctccagtaac gcgctcaacg ccagatgctc acgttcggaa ggcatccacg
    25981 ggacagtacg cacatcggta acacctgcaa tacggcgtat ttcggccatc agactgctga
    26041 aactctcaaa ctccagttca gcaaaattga ggtagattcg cccaatggga tcaatctcaa
    26101 taccgcgtaa atcaatgcct cttagcacga gtagatcgag taattcgcgg gtcagaccga
    26161 gtcggtcttc acaaaagact tccagacgca tgggaacctt cacctgaaaa aagaacaatt
    26221 aatatgatga tatgacacta tttggatagc aggaaggttt ctgtcaacaa tcattgacac
    26281 aaagacggaa aaacgggcac ttcggcgtaa agatatccgg tcagcacccg atattgcatt
    26341 atttttcgct gggcgttttg cctttttgca gcgtttcttt cacctgaccg ataagctgac
    26401 gacggaaatc cccgaggcga ggtttgtcat cgtcaatcca cggcagcggg cggcaaagct
    26461 ccatagcttt aatcccgagt cgtgccgtca gaagtccagc accaattccc tgagctgcgc
    26521 gggtagacaa acgagcagcg agatcttgcg acatccagtc catccccact tcgcgcacca
    26581 gttcgctggc tccggcaaaa gcgatattca gcaataccag cttaaacaga cgcaaacggc
    26641 tgtaataccc cagttcaatg ccatacagcg tggcgatgcg attaattaaa cgcagattgc
    26701 gccaggcgat aaacgccata tcgaccaacg ccagcgggct gaccgcaatc atcaacgttg
    26761 attccgccgc cgaacggctg atttcgcgcc gcgcctgggc atctaaaact ggctggacca
    26821 aatgcgcata caaactgacc acttcacggt cgttttgcgt ttcatggatt gaggcatacc
    26881 agcgttgcag cgctggatga gactgatcaa tacccgcctg ctgcgccagt ttttcgcaaa
    26941 atgcgcggcc tttgcccgtg ccgtggctgt gcaacaaatc gcgcgcttcg tcgcgttcat
    27001 gggcgcgctg tcgcaagcgc cataagcgcc gccactctgt taccacagaa cctacgccag
    27061 cgccgataat caatgcccca gcggcacatc cacccagcgc cacccagtcc tgagtttgcc
    27121 aggcattcat tgtccactgt acaccctgcc cgacaacgct tgcgccaaac agagccagcc
    27181 cgcccatcac cattttgcgc cacaggctgc gtttcggacg taacgctgca tccattaccg
    27241 cttcaacttg cccctcttct tcaggcgctt catcgagcgt ggccggggca aaattttgcg
    27301 cctgattttc gtcaaaggtc tgctgcgccc tgaattttgg attctgatcg acctccagcg
    27361 gaccgtcgaa atcaatacgt ggttttaacg gttcggtcat cgcaatttat ctcctattaa
    27421 aaattcaagc gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat ccatcacctg
    27481 cgggcgaaac gcttcaaact ggaacccttg cttgtcccag aacgcctgac cgggcaaacg
    27541 cgcaggaact tcaccgggat aaacggtgag tggcgcacca tcgctgagcc ggttaccgcg
    27601 caatgccgga attttctcgc cgttgacgtc aataatgccg ctggtggtcg cctgcaccga
    27661 ggccagcccc aggcaatcca tactaatccc ttcaaacgcc gcattttgcc aggcatcctg
    27721 aatcagttgt tgcagtaatg aaaccatatt ggcgtgctga tcgatggtca catggtccgc
    27781 tttagtggca gcaaacaata gcttatcgat aaccggcgaa aacaaacgcc ggaacaaggt
    27841 acgctgcccg tagtggaaac tttgcatcag ctgcgtcagt gccagacgca tatcattaaa
    27901 tgcctgtggc ccactgttga gaggttgcag gcaatcaacc agcacaatct ggcggtcaaa
    27961 gcgcagaaaa tgattcttat agaacccctt caccaccttc tcgcagtaat aattaaaccg
    28021 ctcgcgcagc attccggcat tggtgtgctt atcggcctgc gccagtttgg actcgcccca
    28081 ggcatcgaca tccggccacg ggaagaattg cagcgcgggc gcacctgcca tatctcccgg
    28141 caagacaaag cgcccaggct gaataaagtg cagcccctgc tgtttacagt ggtggagata
    28201 atcggtccac gcggcggcaa tgtctgccag ccggttttcg tcggcaggcg ctagcgggtc
    28261 cagcccttcg cacatcattc gccatttgac cgaccattcg ccgcgctgac cattgagtaa
    28321 gcccgtcatc tggcgtgacc agcttaaata gtcctgcgcc agcatcggca ggtcgagcaa
    28381 ccattcgccg ggataatcca caatctccag atacagcgtg gaggtgtcct tgaagtggcg
    28441 tagcagcgaa tcgttcgatt taaaacgtag cgccaggcgg atttcactga cgccgcgcgt
    28501 tggcgtcggc caggcgggag gatcgccata taactgcgcc agtccttcat catatgtgaa
    28561 gcgcggaatg ccaaagtcac gctgaggaat gcgttttacg cccagcaggc gctcttcacg
    28621 caccgcgctt aacagcggca agcgtgctcc ggcgtgaata ttgagcaact gattgaccat
    28681 cgcagtgata aacgctgttt tgccgctgcg gcttagtccg gttacggcga ggcgcagatg
    28741 tctgtcgaca ccccgattca ccagcgcatt aagttcattt ttaagtcgct tcattgctgt
    28801 cctgtgctgc tctgaagacc agaaaaaaat cattcgtaag gatatgaatg tgggggccag
    28861 gccattgatt atcaactgcc cgtgttgtca tcgacgggcg aactccataa gatgtgcagc
    28921 attcacgttc ggacggagtt cctgacctat gtcccctact atttatgata ttgccagggt
    28981 tgcaggcgta tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacta atatctcccc
    29041 ggaagcgcgc gaaaaagtgt tacgggccat tgaagaatta cagtatcagc caaacaagct
    29101 ggcccgcgcg ctgacctctt cgggttttga tgccattatg gtgatttcta cccgttcgac
    29161 caaaactacg gcgggtaatc cgtttttctc tgaagttttg catgccatca ccgccaaagc
    29221 tgaagaggaa ggtttcgacg tgatattgca gacgtcgcac aatccggcag aagacttaca
    29281 aaaatgtgaa agcaaaatta agcagaaaat gattaaaggc attattatgc taagttcgcc
    29341 agcggatgag tcattttttg cccaacttga taaatacgat attcctgtcg tggtgattgg
    29401 caaagttgaa ggtcaatatg cccatgttta ttctgtcgat accgataatt ttggcgacag
    29461 cattgcgttg accgatgcat taattgaaag tgggcatcaa aatattgcct gcctgcatgc
    29521 tccgcttgat gtccatgttt cagtggatcg ggtaaatggt tataagcaga gcctggctgc
    29581 gcataatatt gcagtgcgtg atgaatggat tgttgacggc ggttataccc atgaaacagc
    29641 actgcaagcc gcgcgggaat tattaagcca gtcaccgttg cctgaggcag tgtttgctac
    29701 tgacagcctg aaattaatga gtatttatcg tgcggcagca gagaaaaata ttgctattcc
    29761 gcagcagtta gcggtggtgg gttatagtaa tgaaacgctg tcgtttattt taacgcctgc
    29821 gccaggcggt attgatatcc caacacagga gttaggtcaa caaagctgtg agttattatt
    29881 ccgcttaatt tctggaaaac cgtcaccaca gaatattacc gttgccacgc atatgacgct
    29941 gaaataaata ccagctacct tgcctgaatg ccggatgcga tgctagcgca tcttatccgg
    30001 cctacattta tagttcggat aaagtactcg tgccgcatcc ggcaatttag cccattatca
    30061 gaacgagtaa tttacgccga cacctgcata atggaattta tcgctgtcgc cttcgtcgtg
    30121 atcctgccac tcaaacgcgt attccagcga aacggaaagc ccgttctgga aatcataacc
    30181 gtaaaataaa cctacacggt taaaatcatg gccttcacgt tcaatatcat cctgccagtc
    30241 ccagttactc cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt ggttgccgag
    30301 cgtcagcggc aaataggcgc gaatttcttg cgtagaaaac tcaccgttat tgcggctgtc
    30361 gtccatatta aagccgcgct cgagataata gttcactcgc aaggcaaccg tttcgttgaa
    30421 ggtatattgc aggcctgttt ccgtttcgac tcgggtatca gcgtaaccgg tcgtgttcag
    30481 atcgttggca aatttataca tcgacaacca accgttgaaa cgtaaatcgt cagtcagttt
    30541 cacatcccag tctggcgcga ttttccagcg ctgcatattc gccgtgtctt tacccggttc
    30601 atcaacgtag tgataaccat aattacggaa accgccggtc aggccgaaac tgaaatcatc
    30661 gttttcgagg aactgataat gcacctccag ctccgggcga tcaaaccacg ttccacgttt
    30721 acccgcgcta taatctaccg gcccttcctg ataataggcc agagcaattc tccacggccc
    30781 gttggcggca ttaaaataga ctgaaggctc cgccagccca tccatatctt cgccataacc
    30841 ctcgacgttt tctatttcgt acatcgcgcc gatattaaag tgccagtcgt tcctttcctc
    30901 ggcctgtgcg caggccattc ccgcacacat caccagtgcg gtacagggta ataacttttt
    30961 cattattgtt ccttgtatta ttctggggga atgccttccc cctgtattta gcgaattgct
    31021 atttccgttt ctgcatcaaa gaaatgacat ttcgtcatat caaaatgaat agtgatattt
    31081 tctcctgcat gataatcatt taacgcacca gcacggacca ctaactcgtg ccccccaacc
    31141 gtggtgtaga gcataaattc cgcaccggtt aattctgcca cgctaatttt ggcggaaatg
    31201 ttattttcct cttgcgcgtc cggatgaata tcttccggac gtattcccat cacgatgggc
    31261 ttatgcaaac ttccctgtgt tttcagaacc gctaatttct cttcgggaat ggttaattta
    31321 agcgtttccg taacgaattt atcgccatcg atcgtgccac gaataaaatt catcgccggt
    31381 gatccaataa atccggcaac aaacatattc gctggttggt tataaaccgt tttcggcgca
    31441 ccaacttgct gaacaatccc atccttcata atcacaatcc gcgtcgccat ggtcatcgct
    31501 tcggtctgat cgtgggtcac gtagatcatg gtggtattca gcttctggtg tagcttgctg
    31561 atctctgcgc gcatttgcac gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt
    31621 aaaaacacgc ccgcttcgcg cacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg
    31681 ccggaaagcg cgcccggctt gcgtttcagg tactcacgca ggccgagaat ttgcgccgcc
    31741 cagttcaccc gctcatcaat cacctctttg gcgatttttt gcatcttcag gccaaacgcc
    31801 atgttgtcgt aaaccgtcat atgcggatac aacgcgtagt tctggaacac catcgctata
    31861 ttgcgtgctt tggctggaac gtcattcatt cgtttgccgt cgatcaacag atcgccgccg
    31921 ctgatctcct caagcccggc aatcatgcgc agggtggtcg acttaccgca gcccgacggg
    31981 ccgacaaaca cgatgaactc tttatcggca atttccaggt tgaagtcctt caccacatgc
    32041 acctggttat cgtagatttt ttgaatatgt tgtaacgaaa gctgagccat gttgattcct
    32101 ttgctatacg ttttgccaga aggccgataa ccgcgaccag gtcagagagt ccgttgaagg
    32161 caacagtaat tgcgccccgg ttaagcccgc gccgatcccc accgagcgca taccgctggc
    32221 gttaatggcg tcaatgcccg cctgcgcatc ttcaatgccg atacatgcct gcggcggcac
    32281 gcccagcccg gcacaggcgg cgagaaagat ttccgggtcc ggtttcgagt ttttaagttg
    32341 ggaagcatcc gcgcagaagg tgaaaaactc gcgcagctcc agcgccgcta aaatcgtcgg
    32401 cgcattcagg gagacagaag ccagcccaac cgggatctgc tgtgcacgga gatctgccag
    32461 caaagagcga atgccgggta gaacagcgct gaccgtcaac tcgcgcagtg agtggacata
    32521 gagcagattt ttgcgatacg ccagttgcgc cctctcctgc gagttaaagt cgccctcttt
    32581 gcccccgtgt tgcagaatgc gccgcagaga ctcatcgcgg ctgatccctt ttagggattc
    32641 gttaaactgc gcatcaatgc tgatgccaat ttcagcggca atctgctgcc acgcctggaa
    32701 atgcagatgc gcggtatcgg tgattacacc atccagatcg aaaattaccc cttgcagttt
    32761 cattcatcct cctgatgtgt ggtagcggtc ccattgaagg gcaaaataaa atcacccaaa
    32821 cagaaaacag attcttcagc cacggatata agctgaccgt tcaaacgcag tgaaacgggc
    32881 gcagaagttc gaatcgcaat acgctgcgcg tcaagagtga cctgtaattc gcagccctgc
    32941 cagaacagag ggaaagacaa ctgttgccac tgctcaggta acgccggatt gagatgcaat
    33001 tcaccgtcac gcacgctcac cccggcaaaa ccctgaatcg cccccagcca gatagcgcca
    33061 gttgcggcag catggatacc atcatcacaa ctatgcggat cagcaccaag atcgatttca
    33121 gtcccctcgc gccagaactg ataactttgg gtcagcaggc cacagcgtgc ggcaacaatg
    33181 ccgtggattg ctttacttaa tgacgagtcg tgaatagtgc gcggttcata aaattgcaga
    33241 ttggcaagac acgatgccgc tgagaactgc tccggcagca tgtaattgag catcaccaca
    33301 tcagcttgtt tgaggatttg catctcgttc acttctgcgc gtgaataatc cagcagaatg
    33361 gtttgcttcc ccgccgccgc tttgtatttc gccagattaa tcgccggctt agccataaac
    33421 gaatcatcct gcggcaaaac gccgtcgggc tgaatttctg gcagccgcag ttctttaagg
    33481 aacatttcgg cgcgatggat aaacgcatcg tcgctacagc caaactggcg ggcaatactc
    33541 agcgcctgct ggacgttgta atacgccata tagctggtga aggcgttatt attgacatgt
    33601 tcggtatatt cgtcaggccc aataacatca tgaatttcca gacggtcgtt aaccctcacc
    33661 gcgcggctaa tccagaactt tgcagtttcc agaagtagcg ccatgccttc atgcgcaatg
    33721 aaactttcat cccccgtggt ctgccagtat tgaataaccg cccaggcgat atcggccacc
    33781 agatgatgtt ccgcctgcgc cgaggccact ttttgccgca gcccggtgcg aatgttaatg
    33841 gcggcaaatt ccggcgtctc ttcttcgccg ctgcgcgcgc tttcccacgg aaatagcgcg
    33901 ccctgccagc cgttgcgtcg cgctttctcc tgcgcgcctg gcaagttgtg ccagcgataa
    33961 cgcagtaaac ttcgggcaac cgtcggatcg ctaaacagat gaaacggtaa caaaaatact
    34021 tctgtatccc agaaaacgtg gcctttgtag ccttcgccgg ttaagccttt tgccgcaatg
    34081 ctgctgcgct cgtcgtgggc aggcgtcatg atgcgcagat gataaagcgc ataatctaac
    34141 gcttgctgat cgtgcgcttc gccgccattt accgtgatac gacgtttctg ccaccattga
    34201 cgccagtttt ctgttgatac tgcaagaagt tggtcgtaac tctgctgcgc gcacatttca
    34261 agctggcgaa gcgacgcgct gccccactcg tctaaaacgg cttgcctgtc atcccgccag
    34321 tcgatccaca ccagtttttg caacgtcact gtctcgcctg catgaagctg cgcactggta
    34381 tgttgctgca aacggcgctc tttggcggta tagcattgct gcacatcacc gctcaccata
    34441 cagcaacagc tgatggccac atcactgcgt ccatcctggg tggtgtagat cccctgcatc
    34501 agatgctgac caaacacccg cacctgggtt tcgtcgagat gttggcgacc gtggttggtt
    34561 tgcgtggcgt cgatacctgt tgaaatcagc actgacgcgt cggcgtccag tggcgtaata
    34621 gtgatttcca gcacaatgag cggcagttgg tctgccgaaa caaagcgacg gctggcgatg
    34681 gtgtaacctg tgccgttgct ggtacgccag acaacgctac ggcgtaattc tccactggca
    34741 aagtcaagtt cacgctgcca ggcttcgcgg gataacgaga aaacctcacc atttatggca
    34801 atctccatcc ccaagatatc aggcaggttc accagttcgt tgatttcacc ttttcccgcc
    34861 cgatgataca gccccgccag atacatccct cgcgtctggc gcgtgtaatc ttcttcatgg
    34921 ctggcgcgaa gcccaagata gccgttcccc tgcgccatca gcgatgcata cttgttcagg
    34981 gtatgctggc tgaaatgggg ttctgataac gttactggcc tggtcataat tccacacgcg
    35041 tccctgtttc tgcggattga tacaacgccg ccaccagttg ctggatgatg tacccctgct
    35101 cggcgtcggc tatcatcacg ggcttgccct gtacgtgatt gataaaggct tccatgctgc
    35161 gcaaatggcg gttgtcgtct gccagttccc gttgcatcag cgtcattaat tcaccgttgt
    35221 tatcggtgta gatatgtgct ggaaacagcg tcgcaccggc tttatcacca caaaagctga
    35281 cgttcataat cgactgttcg cggatgttga gtgcaaatga cgtttccagc cacagaatgc
    35341 cgccgttatg aaattcaatg gtgccaaata acgaatcttc gacgctgtaa gttgccggat
    35401 cccactcgcc aaattgaccg cagctctttt gcgtgccgat cttttgaaag ctatgcgcat
    35461 tcacgctttt caccgccgga aagcccagca catacatcgc cgcatccagc atatgaatgc
    35521 cgatgtcgat aagcgggcca ccaccctgca gttctttatt ggtaaagaca ccccagccgg
    35581 gaacgccgca gcgacgcagg gcgcgggcgg tggtaacgta aatctctccc aaaacgccgt
    35641 tggtcacctg ttcacgcagc tgttgcgtat cgagcgcaaa acggtggtga aagtcgtagg
    35701 ccagcacctt gcccagtttg cgcgcggtat cgcacatttc ccgcgcctgt tctggcgtca
    35761 tggcgggcgg tttttcgcac atcacatggc agcccgcttc cagtgccatc agggtatgtt
    35821 cgtaatgaaa acggttaggt gagcagacgc taaccacatc aggtttcacc gccagcagca
    35881 tggcctgcgg atcgtcccac acggatgcat taccgtattt atctgccagc gcctgcgcct
    35941 gggaaaggcg gctgtcacag acagccacca gttccagatc gttgcgggtg cagtagtacg
    36001 aagcatgaac cttatccgcc acctggcctg cgcctattat cgcgacccgc agcggagagc
    36061 ttgtcattgc acttttcaca aagacctctt agcaggtacg caaccaggcc aacgaatcac
    36121 ggtacgcctg ggcaggctct tccgcccgga tacgcccttc gtacactaca tagccctgat
    36181 agttatccgc gcgcagctgt tcaaacagcg cgtggaaatc cagggtgccg ctgcccggct
    36241 ggtagcgatg gttatccgca atatgcacat gaccgagcag gtcgcggttg tcatgcagcg
    36301 cctgcgccag gttatcttct tcgatattca tgtgatagaa atcgccgata atctgtacat
    36361 gtttaagatc gttttcgacg atgtaacggc gggcatcggc gagggtgttg atcatatgat
    36421 cctgatagcg gtttaacggt tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt
    36481 ccagtacgtg cagggaatca ctcaccattt tgcggtcgcc gtccaggcta cgcggcgagg
    36541 tcatcggcgg taagcggaag gtaaacatgc cccacgctgc cggaacgacg atacctttac
    36601 cccccacttc tgccagcgct tcgagaatgc gttcgatctg ctttaagcca ttaagacgac
    36661 gctcttcgat aaagtcgcca atccaaccgt catagccacc gcaggcggtg gtcaccggta
    36721 aaccggtttc tttgattgct gctttgactt cttcgagatt gttgaccagc agtttgccgt
    36781 caatctcaaa accatcgaag cccatctctt tgatataacg aaatttctcc agaatgtttt
    36841 ccggaaagaa tgcctgattc tgtgtgccta ttttcatttt ttcattcctt tgaattaaaa
    36901 cgtaacgccc attttgatgc tctgttccgg atgctgatcg acatactgca tgtagctttc
    36961 cgggctcttg gcaaaggtca ctaccgggtc gattaaatct tcgcaattga gataaccgtt
    37021 catcagcagt tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg
    37081 gttcggttcg ctgcacgcac gggagaaaac aattttggcg ttattgaaat gcgcttcgcg
    37141 tccgaggtta aaaccttcag caaatggttt ggcaaacgcg acataagaga tggtgccgcc
    37201 ataagcaagg ccacgcagcg ccgattgcag cgcgtcggcg taaccgctgg tttcgataat
    37261 cacatcggca ccctgcttgc cggtcagcgt tttgatctct tttcctacgt cagtgccaat
    37321 ggggttaagg cagaaatcag caccgtggcg acgggcaata tcacagcgat aggcgatagg
    37381 atcgacgcca atcaccaccg acgcgccagc gcgtttcgcc agttggatgg ctatttgacc
    37441 aatcgcgcca agccctacca ccaccacaaa atcacccacg cgcacgttgg catcgcgcac
    37501 gccgctcatg gcaaactgcg ccgggtcgta acaaacggcg tttttccacg agctgccttc
    37561 cggcattttg cgcagcttgt agttattcac cgcgttaatg atgaccgtct cggagagcgg
    37621 gccgtagccg catacgctgt cgcccaccgc gtagtcggta acgtcgctgc cgcactcgat
    37681 aatgtcgcca accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc
    37741 atctgccgga cgcggagtga acatctgcca ttcgccgtta aagtcttcat cgataaacgg
    37801 gctggcggcg cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg
    37861 gattttcact tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacacg
    37921 cggtgctgtg gctactaact ttttcattta aaactccttg taaagaaact ggccgcttta
    37981 gcccttcacg ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa gcgcatacat
    38041 aatcaccacc ggcaatgcgg tcaccagtga ggccgccatc atccgtcccc agatgtaatc
    38101 tggcgtactg aacagcgcgt tcaggcccac cggtaaggtg aaattgctgg cgctggagag
    38161 gaaaatcgac gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacacgga
    38221 tatcagcccg gacatcgcca acggcacggt gatgcggaag ataatttgca gacggttgag
    38281 gccatccatc atcgccgctt cttcgatctc atcggggatg gtgtcgaagt agcttttcag
    38341 cataaacacg gcggtgggca gagtttgcgt caccatggtg atgatcagcg ccatctcggt
    38401 gtcgtaaatg cccagcgcgg tgatgatttt gaacagcggc accaccagca aaatgccaga
    38461 gaacatatac accgtgtaaa agctggcatt gatggtcatc cgacctttaa agcgcaggcg
    38521 ggaaagcgcg taagccccaa gaatgccgag gaataccgcc accacggatg aaaccaccga
    38581 caccaccaga ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc
    38641 gacgtaatgc tccagcgtcc actgctgcgg cagcagcgta ggatgcagtg agatcgcctc
    38701 tttcgcgccc ttgaacgagg tcatcagcat cacaaaaaat ggaaacagcg tgatgatgag
    38761 aaacagcgcc agcccgcagt aaaaaccgat gcgactgagt gtgcgtttat ttgttgccat
    38821 tgaggttcac ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca
    38881 ccgagatcgc cgccgctttt cctaaatcat taaaggcaaa ggcggttttg tagagatata
    38941 caccgagaat atcgaccttg gtcgtcagca aataaacatc cgcgaacatg tagaacatcc
    39001 agatggtgcg cagggtcacc accgtcgcca ggaccggcat aatcgcgggc agcgtgacga
    39061 tacgaaaccg ttgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt
    39121 cgatggtctg caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg
    39181 cgaacagcac caccagcaca aagctactgc ccggattatc gaaccacagc ggtgcctgct
    39241 catagagatg cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca
    39301 tgtatttcca ggcaaacacc agcgaaatgg acggcgttac gtaggagagg atcaccagcg
    39361 aacgcgcggt tttgcgcaga cggaactcgc ggttgaaaaa catcgccact gccagcccga
    39421 gcacggtgct gcccgccacc accagcgcgg tataccagac cgtcatccac agcgagtgcc
    39481 agaagccggg atcggagagg atacgcacat agttactcac cccaacaaag gttgactcga
    39541 tgttgggatt gagcggcaga cgtaaaaaac tgatttcgat attcgacacc attggccagg
    39601 ccaccagacc acccagcagt aataagctgg gcgcgagaag cagcagcgca aagggcatat
    39661 cggaacgacc tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc
    39721 gatcagttca tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc
    39781 cacggtgacg ttatgcacca tggagctcac cacgccagac cccgtcacat cacccatgcg
    39841 ggtaaagttt ttatccccta ctgcaccaaa gacctgaata tttggcagtt caccgattag
    39901 ctgattcggt agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt
    39961 gacaaccgct ttattcaccg gcagcgcagc acctggcgac atcatcaccc agtcggcaat
    40021 gttgtctgcc tgctccataa aggtgacaaa tttttctgct gcttccgtct cttcggtctt
    40081 ttgcccggcg gtaatggtca gcgaggtcaa catgccgtag accgcagagt ttttctcggt
    40141 tggcaccacg aaaccgacgt ttttcgggtc gccttcttta atcacagccg gaaggatata
    40201 ggtggagtaa atcgccatcg gcgcggtgcc gttcataaag gcgtctttca cttccatgat
    40261 gtcgttagaa cccggcatag tgttggcagc aaggtcgcgg taataggtca gtgcctgcat
    40321 catctctggt gtatcaaggg tgattttgcc ttcggcgtta aagacgttag cctggttgga
    40381 taacgcaaac tgggagaagg attgttccgt caacacgctt tctgctgtag gcagcgcaat
    40441 gccgtatttt ttattcgccg ggtcattcag tttctgtgca acgtccagca gctgttgcca
    40501 gtttttcggc tcctcaagcc ccgcttttgc cagcacatct ttgcgatacc agataccgcc
    40561 aatccaggcg ctgacaggaa caccggtcca tgcgctacca tcttcggtac gcacaatacg
    40621 cagtacgcca tcgtaaaacg cgccttcacc aacgttgctg atgactgtgg caaccgcttt
    40681 gcgatcgata agctgctctt tgtccatcac tttggcgtag tcatggctgg tttctatcac
    40741 ttccggcagc gaaccgctac gtgaaagagt aatgacttta gtgttatagg catcttcttc
    40801 cacgggcact tgcttaacgg tgatgccagg gttttctttt tcaaaacggg caatcaattt
    40861 actgataacg gcctggcgct cctgctccac cgaagaatgc ataaattcga tggatacatt
    40921 cgttttattt tcttctttac accctgaaat cagggcgcag gaaaccagtg ctgataacag
    40981 cacgattttt gatttaatca tgagatgttc ctttttattt aatccacata acctgccagg
    41041 ggcgcagcgt tatttcatta cccaatatat caacttcact aatcagatcc cgaccatgca
    41101 gattagtaat atttacatgc tgaatatttt tactgacatt aaacaatcca gtcagacaat
    41161 taccatcagc gttacttctt tgaatacgca ttacggatga attaatcgta tcgatggtaa
    41221 aattattatc cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact
    41281 catgatatac cgcatgcctt aatgtagctt catcgttcag ttctcgggtt atctctttac
    41341 tgtgatattt tttacggtta atcgcacggt tatatccgag tttttcgaca cctgcgtaat
    41401 cattacgcga gccaagaata ctttgaatat atatcgctgg cacaccgggg aaacttaaca
    41461 aaatcgcatg ggcgagaata aacctggcgc aacgttcttc atcgctactc tcacggcggc
    41521 ttaacgcatc catataggtc acatttattt catacggact gcgtgtaccg tcgggattat
    41581 ttttccagtt tactaatgca ccttcctgct gtaacgcctc gaccagctct aatatttcgc
    41641 tttcaggcaa caagccccgt agcgggttta gcccgatgcc atcgtgagag gcgaggaagt
    41701 taaaccaggt ggtgttgctg gaaggtagtg tcaggttttg cgcccacgca caaagcgcct
    41761 caacgttctg tttttgcacc gcatgcagca ccagtggcgg cagcgagaac tggtacacca
    41821 tatgtgcttc gtcatcgcct gcgccaaagt aagcaatgtt gtctttatgc ggaacattgg
    41881 tctcggtaat gatcactgta cctggcgcaa cgttatcaat aatcgaccgt aacagtttga
    41941 taatcagatg tgttttttcc agatggatgc agcttgttcc cggctctttc cacataaagc
    42001 caacggcatc caggcggaca tactcagcac ctttcgcaag gtaacagagt aaaacatcca
    42061 ccatcgccag caacacttca gggctacggt aattcaggtc aatttgatcg tcactaaagg
    42121 tggtccataa atggcgcgtt gaatgatcgc gcatctggaa tggcgttaat aacggtaacg
    42181 cacgcgggcg agtgacggcg ctgagatcgg tttgcgggtc aacggcaata aaaaaatctt
    42241 caaaacctgg atgctgttgt aaatagtttt taaatcattc actttttgcc gacatatggt
    42301 tgcagacaaa atcaaacatt aaatgactgc attcaccgag ttgctgaata tcctgccact
    42361 cccccgcttc actggcgacc tgatgataat caattaccga aaagccatca tcagatgacc
    42421 atggataaaa cggcaacaaa tgaacatgtg aaaaaatgct ttgcagccat tgatggtaaa
    42481 actgattaaa tgtgggtaat ggttttaaat cattgctgtg aaattgatcg gcataggtaa
    42541 ttaacacgac atcactttca tcccagtgtt ttttacgtcg ctgtgtaatt aatcgtttcg
    42601 cactctcaag acgcgttaca agtttctgta aatgagttgc ggtaaatgtt ccaccgtaga
    42661 tttcgtccag gtaatccgta attttctgtt tcatgttgcc tttccgtggt agcggtccca
    42721 tgaaacgaga gactactccg cagaaaataa actgtcaatt gagcacaagg gttttagtca
    42781 cgcaggaaat cattttttac cagggaaaaa gcgtatttgt gagctgacgc aaataatcta
    42841 tggggatgta aataaggtgt gacagaaaaa aaacggcgca taagcgccgc tcatggtgaa
    42901 ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccaccggc gttctcaacg
    42961 tgggtatatc ccatctcgct aaggatctct tttgcttgcc ctgactggcg tccggcattg
    43021 caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg ctctttcact
    43081 tccttcaggg gaatattgat ggccccctga acgtgctctt gctgatactg ctctggaaca
    43141 cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag tgccagagca
    43201 agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
    43261 cgcctgagat ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt
    43321 tattagcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
    43381 ggcgagcaac tgattttatc gcccagcccg ctacacccgc cgggccatag cgcagtgcgg
    43441 taagaagtac cagcttgcct gctaatttga aaccaggctt taccttttgc ccggcctgtt
    43501 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
    43561 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
    43621 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
    43681 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
    43741 accgaagaaa atcgacagca ccaccaggat acgcaccagt tttgccggta catcaaaata
    43801 gttggcaatc ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataattt
    43861 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
    43921 atagattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcagc cagttgcgct
    43981 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
    44041 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
    44101 gcgctcataa gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
    44161 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat
    44221 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
    44281 tttctgcttc cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg
    44341 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
    44401 gatgacgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
    44461 caccgatctc tttcttcatg cgtgccagcg tgtcgtccac cagcgtcact tcatgttcca
    44521 ggctcttaat cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct
    44581 ctctctcttt cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg
    44641 cttgttcaat acggcgagtc agctgtttct tttctgccaa cgcgcgcgcc gaagtagaac
    44701 gtacttcaac cagtgtatct tccatctcct ggatcatcag acgaaccagt ttctgtggat
    44761 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
    44821 aaatacccat aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta
    44881 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaacca
    44941 acagcaacat gccaggatga gttagcgaat tacactaaca agtggcgaat ttcatcatgg
    45001 cagaatacaa agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg
    45061 tttcgcatct cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta
    45121 aagagctgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
    45181 cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag
    45241 cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgacggcg
    45301 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag aaattattgc
    45361 gcgtgattga gtacggtgaa ctggagcgcg ttggcggcag ccagccattg caggtgaatg
    45421 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggcacttttc
    45481 gcgctgacct gctcgaccga ctggcttttg atgttgtaca actgccacca ctgcgcgagc
    45541 gcgaaagcga cataatgttg atggcagaac actttgccat ccagatgtgt cgggaaatta
    45601 agctgcctct gttcccgggg tttacagagc gcgccagaga aacattgctg aattatcgtt
    45661 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
    45721 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccgcctgaag
    45781 acgctatcgc cgtttcagaa accacctcgc ttccaacact gccgctggat ttacgtgagt
    45841 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacaaggg aaatataacc
    45901 agaagcgtgc ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa
    45961 agcaccagat ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat
    46021 tcggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta
    46081 ttgcgttggg ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt
    46141 gcgcatccgg tatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg
    46201 tcagcaggag cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg
    46261 acggctcgcc cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta
    46321 ccgcagcaat ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc
    46381 ccagttgatt cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg
    46441 cggcagccac cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg
    46501 cgtccataat attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt
    46561 tcgccatcgt cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg
    46621 tacgcgcaaa gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca
    46681 ggcggcgaat ggcggcaacc agctcttttg gcgcaacgtt gaaaccgccc tcgccctgca
    46741 ccggttcgaa aataatggcc gcgacctgct tcgcttcgat gtctgattta aacaagcgtt
    46801 cgatggcgtc gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt
    46861 gatacaccga accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca
    46921 gcgccatggt catatatgta cgaccgtgga agccaccgct aaacgcaatc acgccaggtc
    46981 gtccggtatg ggcgcgagca attttcaccg cgttttccac cgcttccgca ccggtagtga
    47041 agaatgcggt tttagcctgc ccgctcactg gggcaagggc gttaattttc tccgccagcg
    47101 tgacgtagct ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct
    47161 gctccaccgc cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg
    47221 cggcgaaatc gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact
    47281 gggcgaagaa gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgcgctgatg
    47341 gaattcattg ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa
    47401 cgcccagacg atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaat ggcacgcgca
    47461 gcgtgcgccc gccggggaac gggtaatgcg gcagattggc gaaggcatcg aaacgttcgg
    47521 cgtcgccgcg cagcagttcg gcaatcagac gcccggcaag atgggtacag gtgacgccgt
    47581 ggccgctgta gccctgcatg taatagatgt tggtatcaag gcgaccgaac tgcggcatgc
    47641 gcgacaaggt cagcaggaag ttgcccgtcc agcggtaatc aattttcacg cccttcagct
    47701 gcgggaaggt tttcagcagt ttcggcacca caaggcgctc aacgtcatcc gggtcgcgcg
    47761 cgccgtagac cacgccgccg ccgtacagca ggcggttgtc ggcggtaaga cggtagtaat
    47821 caagcagata gttacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat
    47881 cttccgacag ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg cgtttcgcca
    47941 gttccggctc tactttatcg cccagatacg cattcccggc gacaatcaca tacttcgccg
    48001 tcacctgacc tttggcagtt cgcacaacgg ctggcgtggt gtgctggatc tgcgtcacgg
    48061 cagaaagttc atacacgcgc ccgccgttga ggcggatggc gtccgcttcg ccaatggcga
    48121 ggtttagcgg atgaatatgc ccaccgctgt gatccagcag cgcaccggta tagcgatcgc
    48181 tggctacttc acggcgaatg gcgttggcgt ccagcaattc cagctgttta ttgccgtagc
    48241 gttcccagtt ctctttctgc tcttcaagtg tggcgagctg tttatcattc atcgccacaa
    48301 acaggccgcc ggggcggtag tcgcagtcaa tctgataacg tttgatacgt tcgcggatga
    48361 tctcaccacc ttcgaacatc atgctgccga gcatacgggc ggtgtccatg ccgtagcttt
    48421 tttcgatcac gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca ttgcgcccgc
    48481 ttgcgccaaa gccgatgcgt gaggcttcga gaaccactac gtcaaagccc gcttccgcca
    48541 gatgcagcgc ggaagagagc ccggtatagc cgccgccgac cacgcaaacg tcgcaggaaa
    48601 tcgactcatt cagcgtgtcg aatggcgcgt atttattcgc actggcggcg tagtaactgc
    48661 tggtatgttc ggtcatttca ggcctccagg cttatccaga tggttttcag ttcggtgaat
    48721 ttttcaaggg catgcaggga tttgtcgcga ccgttgccgc tctgcttata gccgccaaac
    48781 ggcacggtca tatcgccgtc gttgtagtta ttgacgaaga cggaaccagc cttcaggcgg
    48841 cggctcatgc ggtgcgcgcg ggagaggtcg cgcgtccata ccgccgcgcc aaggccgtac
    48901 tggctgtcgt tggcaagctg tagcgcctgg tcttctgatg tgaaacgcgt gaccaccagc
    48961 accggaccga aaatctcttc gcggcttaag gacgcattcg ggtctacatc cacaaagatg
    49021 gtcgggccga tggcggcagc cagctcagcg ttacgaccat ccagcagcag ttgccctttg
    49081 ctttcgcctt cccgaataaa gctatggacc gagtcagcgt gagcgctgtc gattaaggtg
    49141 cccatggtgg ttgcgggatc aagtggatgg cccggctgcc agttttgcgc ctgctgtttt
    49201 aacagggcta agaattcatc ggcgatgctc tcttccagta acaaacgcgt tccggcgatg
    49261 cacacctggc cctggttgta gaagatgcct gcggcggtgg cgcttgccgc tttttgcaga
    49321 tccggacaat cggcgaaaac gatgttggcg cttttgccgc ccgcttccag ccagacgcgt
    49381 ttcatgttgc tctcgcccgc atctttcagc agctgtttcc cggtacgggt cgaaccggta
    49441 aaggcaatgg cgtcgatatc gttatgacgc gacagcgcct gccccgcttc atgaccaaaa
    49501 cctgtcacca cgttcaaaac accatccggc aagcctgctt ctttcgccag ccccgcgaga
    49561 cgaatcgcac tgagcggtga tttttcagac ggtttcagga caacgctgtt ccctgctgcc
    49621 agcgccgggc caagcttcca gcaggtcagc agcagcggaa agttccacgg tacgatggcg
    49681 gcaatcacac cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc
    49741 acttcgccat acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg
    49801 ggaatatcat cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt ttccagcagt
    49861 gccagctctt cggcgtgggc ttccattaaa tcggtgagtt tattcagtac cgctttacgt
    49921 ttagccggag aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc tgcgctcacc
    49981 gcacggtcga tatcgacgct cttgccgcgg gcaattttcg ccagcggtgc ctgggtgacc
    50041 ggatcaacgg tttcaaaggt ttcattttcc gccgcagcag tatattcacc gttaataaat
    50101 aagcgggttt caatggcgag acttaacgct ttatcctgcc agtaagccag atgatgaaaa
    50161 ttcattatga ctcctgtttc acgtctatca gatatatgca gtttgtgaac gtcagggcgc
    50221 agagatcccc gggccgtatg cccgtttatt tctggcgaca ttctttatat acacttcatc
    50281 cttcaagccg cctctgcctt ggctgcgttc gctcccccga atcacttacc tgagtaaact
    50341 catcgggatt cgctcacttg ccgccttgat gcaacttgaa tgattttgtg tatagattgc
    50401 cgggcgcatt gcgcttaccc ggcttacaga acaaaaagat taaaacgtgg tgggcgtatg
    50461 ggcgctgata attcggcaaa tacctgccga cgtattactg aaactgtgcg ggatgccggt
    50521 attaatggca taactttgcc ccgcgacgag gtggtaatcc tgaccattaa tcgtcagaac
    50581 aatttcacct tccagtactg tgcctatttc ctcaccctga tgcttaattc tttccccagt
    50641 ggttgtgccc ggctggtacg tttcaaagat catcgccagc gtgcgattcg ggttaccgtt
    50701 atgaaccagc ttcattgaca caccctgact gcccatctca attaagtcgt cctgattaat
    50761 gacgacctgc ggctcatcag gtttttccgg ctcggaaaag aattccgaga gtgacagacc
    50821 atacaccttc agcagctttt gcagcgtact gatggcaggg ctgactttat cttgttctat
    50881 cgtactgata gcactgtgag tcagcccgga gagttcggcg gcacgacgtt gtgaaagccc
    50941 ctgctgctgg cggatttccg acaagcgttt tcctggcgcc agtccctcat cactcatatt
    51001 tgcatttcct taaactgtag tggtcagagt cgctgttttt cagcgatatg gtgctgacaa
    51061 gcggtgataa agccctcgaa caatatacgc gaaagcgcgt actcgctact gttccattcc
    51121 gggtgccact gtacgcccag cgcaaaagga tgattgatga cgctaaccgc ctcaactaag
    51181 ccatccggcg agcgagcttc aacacgcaac cgtgggctaa cgaccttcgc cccttgccca
    51241 tgcagagagt ttacccaaaa gttgctacat tcaggtaaca acgcagacag taatcccccc
    51301 tcttcaacct gaacttcatg cgacggtgca tattgctgtt ccaccggcag ttcgggatct
    51361 tcccgatgtt ccagcaattc aggctgttcg cacagcttgc gatgcaacga accaccggtt
    51421 gccaccacca gttcctgtaa accccggcag atggcgaaaa tggggatgcg cctttcgagt
    51481 gcggcattaa ttaacgccat gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg
    51541 ccgttttcac catataggtg cggctgcaca ttgctgggac taccaggaag ataaatgcca
    51601 tcgagtttcg gcaaaagttg ttcaagtaat gacggttccg ccagcgcatg tggtagcgca
    51661 attggcaagc cgcctgcgtg gatgatggca ttcaggtact tttcttgcag agtctgggtc
    51721 gcatgaccct taagcctgtt cctgcacatt acgacaccga taaccggatt gttcattata
    51781 ttttccatgc tcaatctcac aaagtggact aaattatcgc cattactgcg gtttaaatga
    51841 cctttatgtt caatattttt tcaatctagc agtgggtttt catttttgca aactcaattt
    51901 aacatttgac aaacatttag tttgcataca gattcgaatg gtggtcatta tattttacgc
    51961 tttgataacg agcggaaaac aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa
    52021 gtagagaact ttgttcagca gtcagaagag aggcggggta gcgcctttac gcaggaagtg
    52081 aaacgctacc tggagcgcta cccgaatacg caatatgttg atgttttact tactgatttg
    52141 aatggttgct tccgtggaaa acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg
    52201 tgttatttcc cggcctcggt gtttgcaatg gatattctgg gcaacgtagt agaagaagcg
    52261 ggtctgggtc aggaaatggg cgagccggat cgtacttgtg ttcctgttct cggttcctta
    52321 accccttctg ccgccgatcc agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa
    52381 gatggcgctc cctttgacgt tgagccgcgg aacgttctta accgcctgtg gcagcagcta
    52441 cgccagcgcg gattgttccc ggttgtagcg gtagagctgg agttctattt actggatcgt
    52501 cagcgcgacg ctgaagggta tctgcaaccg ccctgcgcgc ccggcaccga tgaccgcaat
    52561 acacaaagcc aggtttactc cgttgataac ctcaaccact tcgccgatgt gctcaatgat
    52621 attgatgaac tggcgcagtt acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg
    52681 ccgggtcagt ttgaaatcaa cctttaccat accgataacg tgctggaagc ctgcgatgat
    52741 gcgctggcgc taaaacgcct ggtgcgtctg atggcagaaa agcataagat gcacgccact
    52801 tttatggcga agccgtatga agagcacgcg ggcagcggaa tgcatatcca tatcagtatg
    52861 caaaataacc gtggcgagaa cgtgctttct gacgcggaag gcgaagattc gccgctgctg
    52921 aaaaagatgc tcgccgggat gattgacctg atgccgtcgt cgatggcgtt gctggcacca
    52981 aacgtgaact cgtatcgccg cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg
    53041 ggccataaca accgcaccgt cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc
    53101 gtggaatatc gcgtggcggg tgccgatgcc aacccatatc tggtgatggc agcgattttt
    53161 gccggtattt tgcatggcct tgataacgag ctgccgttgc aggaagaagt cgaaggcaac
    53221 gggctggaac aggaaggctt acccttcccg attcgccaga gcgatgccct gggtgagttt
    53281 atcgagaatg atcacctgcg ccgctattta ggcgaacggt tttgccatgt gtatcacgcc
    53341 tgtaaaaatg atgagctgtt gcagtttgag cgcctcatca cagaaacaga aattgagtgg
    53401 atgttgaaaa acgcgtaatt ccccatgcgg ggttgtttca tcggcctgag cggagccagg
    53461 cctgacattt gctggtaatc cagacatttc ccggatgtcg cgttatggag cgcaggcggc
    53521 aacgggcttc cgttaacgat taagactgtg acgaggaaat gagatgatgc cgatgattaa
    53581 atccccgcat ggcgagggag gctgcgtatg cgcccctcct gctactgact ggactccgcc
    53641 ccctttgctt cccttgctaa acaggtttga tttcaggtcg acacgaccgc aaaccttatt
    53701 acgcagggga ggcagcaatt atggctatta attcaccact gaatattgct gcgcaacccg
    53761 gcaaaacccg tctgcgaaaa tcactgaaat tgtggcaggt ggtaatgatg ggtctggcct
    53821 atctcacgcc gatgaccgta tttgatactt ttggtattgt ctccggcatt agcgacggtc
    53881 acgttccagc gtcctatttg ctggcgctgg cgggcgtgct gtttaccgct atcagctacg
    53941 gcaaactggt tcgccagttt ccggaggccg gttcggccta tacctacgcg caaaagtcga
    54001 ttaatccgca cgtcggattt atggtcggct ggtcatcact gctggattat ctctttttgc
    54061 caatgatcaa cgttttgttg gcgaaaatct atctctccgc cctcttcccg gaagtgccgc
    54121 cgtgggtgtg ggtggtaacc ttcgtcgcca ttttaaccgc cgcgaatctg aagagcgtca
    54181 acctggtcgc taacttcaat accctgtttg tactggtgca aatctccatc atggtggtgt
    54241 ttatcttcct ggtggttcag ggactgcata aaggagaagg cgttggcacc gtctggtcac
    54301 ttcagccgtt tatcagcgag aacgcgcacc tgatcccgat tattaccggt gcgacgattg
    54361 tctgtttctc gttcctcggt ttcgatgcgg tgaccacgct ttcggaagag acgccggacg
    54421 ccgcacgcgt gatcccgaaa gccatcttcc tgacggcggt atacggcggc gttatcttta
    54481 tcgcggcgtc gttctttatg cagctgttct ttcccgatat cagccgcttt aaagacccgg
    54541 acgccgcact gcctgaaatt gcgctgttcg tcggcggcaa gctctttcag tcgattttcc
    54601 tctgcaccac gtttgtgaac acattagcgt ctggcctggc ttcacatgcc agcgtgtcgc
    54661 gtctgctgta tgtgatgggg cgtgacaatg tgtttccgga gcgcgtgttt ggctatgtgc
    54721 acccaaaatg gcggactccg gcactcaacg tcattatggt cgggattgtc gcgctgtcgg
    54781 cgctgttctt tgatttagtc accgcgacag cactgattaa cttcggtgct ctggtggcgt
    54841 ttaccttcgt gaatctgtcg gtgtttaatc atttctggcg gcgtaaagga atgaataaaa
    54901 gctggaagga tcacttccac tatttgctga tgccgctggt tggcgcgctg acggtgggtg
    54961 tgctgtgggt taacctagag tcaacgtcac tgacactcgg tctggtatgg gcttcgttag
    55021 gcggcgcata tttgtggtat ctgatccgcc gctatcgcaa agtgccgctg tacgaaggtg
    55081 acagaacgcc ggtgagcgaa acgtaatagc cggatgcgcg gcgcattatc ctgcctgagg
    55141 gtaacgcgcc gcgccattaa ctataaaaag atgtatgacc cggatcacga gtgctatcat
    55201 ccgcccttat gcttagcgca ttaaccccag gggcaaataa tgaaccacga tatcccgcta
    55261 aaatattttg atattgccga tgagtacgcg accgaatgcg cagagcctgt cgcagaggct
    55321 gaacgcacac cgctggccca ctacttccag ttactgctca cccgtttgat gaacaatgaa
    55381 gagatcagcg aagaagccca acatgaaatg gctgctgaag cggggattaa tcccgtacgc
    55441 attgatgaga tcgcagagtt tttgaatcaa tggggtaatg agtaattatt taattgcaga
    55501 cgatcggtca gtatcgttgt ccggataaag cgttcacgcc gcatccggct aacaatgaca
    55561 cgcactgcag cgtgctttaa tgggtttctc acgatcgtac cgctctgctc ctcgctaagt
    55621 tgttctgtga gttatagtta tagagaaatc acgggtacga atgttgaaca gatggcgacg
    55681 ccacctgttc aacgaccaac cacacggccc tcattacgac ccctgaacac aaacggtaat
    55741 ggcaaatgtc attaaatatt agtaagatag cctctttaca taaaatccct taatattatg
    55801 cgccaggtat tatcgtctct tttggtgatt gctggacttg tgagtggtca ggcaatcgcc
    55861 gcgcctgaat cccccccgca tgctgatatc cgcgacagcg gttttgtcta ttgcgtcagc
    55921 gggcaagtca acacctttaa cccatccaaa gcgagcagtg ggttaattgt cgataccctt
    55981 gccgcccagt tttatgatcg actgctggat gtcgatccct atacctatcg cctgatgccg
    56041 gaacttgccg aaagctggga agtactcgac aacggcgcga cctatcgctt ccacctgcgt
    56101 cgcgatgttc cgtttcaaaa aaccgactgg tttactccca ctcgtaaaat gaatgccgac
    56161 gatgtggtgt ttaccttcca gcgaattttt gaccgcaaca acccgtggca taacgtcaac
    56221 ggcagcaact tcccctactt cgacagcctg caatttgccg ataacgtgaa aagcgtccgc
    56281 aaactggata atcataccgt tgagttccga ctcgcccagc cggatgcttc ttttttgtgg
    56341 cacctcgcaa cccattatgc ttcggtcatg tcggcagaat atgcccggaa gttagagaaa
    56401 gaagatcgcc aggagcaact cgaccgtcaa ccggtcggca ccggtccgta tcagttgtcg
    56461 gaataccgcg ccgggcaatt tattcgccta caacgtcatg atgacttctg gcgcggtaaa
    56521 ccgttaatgc cgcaggtagt ggtggattta ggctccggcg gcaccggacg tctgtcgaaa
    56581 ctcctgaccg gggaatgcga cgttctggcc tggcctgctg ccagccagct atccattttg
    56641 cgtgacgacc cgcgcttgcg tttaacgctg cgtcctggga tgaacgtcgc ctatctggca
    56701 tttaacaccg ccaaaccgcc gctaaataat cccgctgtcc gccatgcact ggcactggcg
    56761 attaataacc agcgcctgat gcaatccatc tattatggta cggctgaaac ggcggcctct
    56821 attttaccgc gcgcctcgtg ggcctatgac aacgaggcta aaattactga atacaatccg
    56881 gcgaaatcgc gcgaacagtt gaaggcgttg gggctggaaa acttaacgct gaaactgtgg
    56941 gtgcccacac gttcgcaggc gtggaacccc agtccactga aaactgccga actgattcag
    57001 gcggatatgg cgcaggttgg cgtaaaagtg gtgattgtgc cggtagaagg tcgctttcag
    57061 gaggcgcggt tgatggatat gagccatgat ctgacgttat ccggttgggc gacggacagt
    57121 aacgacccgg acagtttctt ccgtccttta ctgagctgcg cggcaattca ttcacagacc
    57181 aacctcgccc actggtgcga tccgaaattc gacagcgtgt tgcgtaaggc gctctcctcg
    57241 cagcagctgg cggcgcgtat tgaagcctat gacgaagcgc agagtattct ggcgcaggaa
    57301 ttgcccattt tgccgctggc gtcgtcattg cgtttgcagg cctatcggta cgatatcaaa
    57361 ggtctggtac ttagcccgtt tggtaacgcc tcctttgctg gggtgtatcg cgagaaacag
    57421 gatgaggtga aaaaaccatg attatcttca ccttacgccg cattttgtta ttgattgtca
    57481 ccttgttcct gctgaccttc gttggcttca gcttgagcta tttcacccct catgcgccgt
    57541 tacaaggcgc gtcactgtgg aatgcctggg tgttctggtt taacggcctg atccactggg
    57601 attttggtgt ttccagcatt aacggtcaac cgattgccga acagttaaaa gaggtcttcc
    57661 cggcgacgat ggaactgtgc atcctcgcct tcggttttgc gctgattgtt ggcatccccg
    57721 tggggatgat tgccgggatt acgcgccata agtggcagga taacttgatc aacgccatcg
    57781 ccttactggg cttttcaatc ccggtgttct ggctggcgct tctgttgacg ctgttttgtt
    57841 cactcacgct aggttggttg cccgtttcag ggcgtttcga tctgctctac gaagtgaaac
    57901 cgattaccgg ttttgcgttg attgatgcct ggctttcgga ctcaccatgg cgggatgaaa
    57961 tgatcatgag cgcaatccgc catatgatat tgcccgtgat caccctgtcg gttgcgccaa
    58021 caactgaagt gatccgcctg atgcgtatca gcactatcga agtgtatgac cagaactatg
    58081 taaaagcggc ggcgacccgc ggtttgtcgc gctttactat tttgcgtcgc cacgttctgc
    58141 ataacgcgtt accgccggtt attcctcgcc tgggcttaca gttttccacc atgctgacgc
    58201 tggcgatgat caccgaaatg gtttttagct ggccgggttt agggcgctgg ttaattaacg
    58261 ccatccgcca gcaggattac gcagccattt ccgccggagt gatggtgtgt ggctcactgg
    58321 tcattattgt taacgtgatt tctgatattt tgggtgccat ggctaaccct ctgaaacata
    58381 aggaatggta tgccttacga tagcgtatac agcgaaaagc gcccgccggg cacgctgcgt
    58441 accgcctggc gcaaatttta tagtgacgcc tctgcgatgg tcggcctgta cggctgcgcg
    58501 gggctggctg tactgtgtat ttttggcggc tggtttgcgc cttacggcat cgaccagcaa
    58561 tttctcggtt atcaattatt gccgccgtca tggtcgcgct atggcgaagt ttctttcttc
    58621 ctggggactg acgacctcgg acgcgatgtt ttaagccgtt tactgagcgg agctgcgcca
    58681 accgtgggtg gcgcatttgt ggtgacgctt gccgcgacga tctgtggcct ggtactgggc
    58741 accttcgccg gggcgacaca cggcctccgc tcagcggtgc ttaaccatat tctggatacc
    58801 ttactggcga tcccttcgct gctgttggca attatcgttg tagcgtttgc cggaccgagt
    58861 ttgtcccacg ctatgtttgc tgtctggctg gcattgctgc cgcgtatggt acgttcgatt
    58921 tacagcatgg tgcatgacga actggaaaaa gagtacgtta tcgccgcccg tctggatggc
    58981 gcatcaacgc tgaatattct ctggtttgct gtgatgccaa acatcaccgc cgggctggtc
    59041 accgagatca cccgcgcact gtcgatggca attctcgata tcgccgcgct gggctttctc
    59101 gatctcggcg cacaactccc ctcgcctgaa tggggagcga tgctcggtga tgcgctggaa
    59161 ctgatttatg tcgccccgtg gactgtcatg ctgccaggtg cggcaattat gattagcgtg
    59221 ttgttggtta acctccttgg cgatggcgtt cgtcgtgcga ttattgcggg ggtggaataa
    59281 tgccattact cgatattcgt aacctgacca ttgaatttaa aaccggtgat gagtgggtta
    59341 aagccgtcga ccgggtaagt atgaccttaa ccgaaggtga aatccgcggt cttgttggtg
    59401 aatccggttc cggcaagagt ttgattgcga aagcgatttg tggggtgaat aaagataact
    59461 ggcgtgttac tgctgaccgt atgcgttttg atgatatcga tttgctgcgt ctctccgcac
    59521 gcgaacggcg caaactggtt ggccataacg tgtcgatgat tttccaggaa ccgcagtcgt
    59581 gtcttgaccc ttcagaacgt gtgggtcgcc agttgatgca aaacatccca gcctggacct
    59641 ataaaggccg ttggtggcag cgttttggct ggcgcaaacg ccgtgccatt gaactgctgc
    59701 accgcgtggg gattaaagat cacaaagatg cgatgcgcag ttttccctat gagttgaccg
    59761 aaggtgaatg tcagaaagtg atgatagcca ttgcactggc gaatcaaccg cgtctgctga
    59821 ttgctgacga accgaccaac tcaatggagc caacaaccca ggcgcaaatc tttcgcctgc
    59881 tgacgcgtct caaccaaaac agcaatacca ctattttgct tatcagccat gacttacaaa
    59941 tgcttagcca atgggcggat aaaattaacg tgctttactg cggtcaaaca gtggaaaccg
    60001 cgccgagtaa ggagttggtg acgatgccac atcatcctta tacccaggcg ctgatccgcg
    60061 cgataccaga cttcggcagc gcgatgccgc ataaaagtcg cctcaatacg ctgcccggcg
    60121 ctatcccact gctggaacag ttaccgattg gttgtcgtct ggggccacgt tgcccgtatg
    60181 cacaacgaga atgcattgtg acgccacgtt tgacgggggc gaaaaatcat ctctatgcct
    60241 gtcatttccc gctgaacatg gagaaagagt gagatgatcg aaacgctgct tgaagtgcgt
    60301 aatttaagta aaacgttccg ctaccggacc ggctggtttc gtcgtcagac cgtagaagcg
    60361 gtaaaaccct tgagctttac gctacgtgaa ggccagacac tggcgattat tggcgagaat
    60421 ggttcgggta aatccacgct ggcaaaaatg ctggcgggaa tgatagagcc taccagcggc
    60481 gagttattga ttgacgatca tccactgcat tttggcgatt attccttccg cagtcagcgt
    60541 attcgcatga tttttcagga tccttcgacc tcgttgaatc cccgtcaacg tatctcgcaa
    60601 attctggatt ttccactgcg cctgaacacc gacctggaac cggaacagcg tcgcaaacag
    60661 attattgaaa cgatgcgtat ggttgggctg ctaccggatc acgtcagtta ctatccgcat
    60721 atgttggcac ccggacaaaa acagcgtctg ggtctggcgc gcgcgctgat attgcgccca
    60781 aaagtcatta ttgccgatga agccctcgct tcactggata tgtcgatgcg ttcgcagttg
    60841 attaatctga tgctggagtt acaggaaaaa cagggcattt cgtatattta tgttacccag
    60901 catatcggaa tgatgaagca cattagcgac caggtgctgg tgatgcatca gggcgaggtt
    60961 gtcgaacgag gcagcaccgc ggatgtgctg gcatcgccgc tgcatgaact caccaaacgg
    61021 ctgattgccg ggcactttgg cgaagcattg acagcggatg cgtggcgtaa agatcgctaa
    61081 ttttctgaat cctccttcca gattatccac ctcattctga caagagatta aatcttgtca
    61141 gaatgaggtg atggataaaa taaaatcaaa tgcgcgtgat ttaagacgca atctcacttt
    61201 gcaggaacga aagctctggc gatatcttcg cagccgacgt tttggtgatt tcaaatttcg
    61261 ccgtcaacat ccggtgggga gctacattct cgattttgct tgctgctcgg cgcgcgtagt
    61321 cgttgagctg gatggtgggc agcatgattt ggcagttgcc tatgatacca ggcgcactag
    61381 ctggcttgag tcgcagagct ggaccgtgct gcgtttctgg cataacgaga ttgattgtaa
    61441 tgaggaggcg gtgctggaga ttattctgca ggaactgaac cgccggtcac cctcaccctg
    61501 aaagggcgag ggggccgacc gagccgaata gctgttgtgg tgaaaacatg gagacagtgc
    61561 tggagaatat tcggcaaggt ctgaaccatc ccagccatcg ccataaaagg gttaggggct
    61621 gtatgagcct gtttgttgct ggggtaacaa tatttgcaca atatggtccc ctcgcccctc
    61681 tggggagagg gttagggtaa ggggaaaagc gcccaccctg ccgcagcctg ctccggtcgg
    61741 acctggcaac tatagctact cacagccagg ttgattataa taaccgttta tctgttcgta
    61801 ctgtttacta aaacgacgaa tcgcctgatt ttcaggcaca acaagcatca acaataagga
    61861 ttaaagctat gggttttctt tccggtaagc gcattctggt aaccggtgtt gccagcaaac
    61921 tatccatcgc ctacggtatc gctcaggcga tgcaccgcga aggagctgaa ctggcattca
    61981 cctaccagaa cgacaaactg aaaggccgcg tagaagaatt tgccgctcaa ttgggttctg
    62041 acatcgttct gcagtgcgat gttgcagaag atgccagcat cgacaccatg ttcgctgaac
    62101 tggggaaagt ttggccgaaa tttgacggtt tcgtacactc tattggtttt gcacctggcg
    62161 atcagctgga tggtgactat gttaacgccg ttacccgtga aggcttcaaa attgcccacg
    62221 acatcagctc ctacagcttc gttgcaatgg caaaagcttg ccgctccatg ctgaatccgg
    62281 gttctgccct gctgaccctt tcctaccttg gcgctgagcg cgctatcccg aactacaacg
    62341 ttatgggtct ggcaaaagcg tctctggaag cgaacgtgcg ctatatggcg aacgcgatgg
    62401 gtccggaagg tgtgcgtgtt aacgccatct ctgctggtcc gatccgtact ctggcggcct
    62461 ccggtatcaa agacttccgc aaaatgctgg ctcattgcga agccgttacc ccgattcgcc
    62521 gtaccgttac tattgaagat gtgggtaact ccgcggcatt cctgtgctcc gatctctctg
    62581 ccggtatctc cggtgaagtg gtccacgttg acggcggttt cagcatcgct gcaatgaacg
    62641 aactcgaatt gaaataatca ttctgttggt aaatattggg cggcgttctg ccgcccgtta
    62701 tctctgttat acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct
    62761 tgcgtcagga taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca
    62821 ccggcaaaag ccactggtat catgaaacgc aatccagtac tgcggagtat gacgttctgc
    62881 ctctggtccc ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg
    62941 aagaaacgct ggctcccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggaat
    63001 tgttccctga ccagcttacc gtgacccgtt cacagacctt caccgcttat gaacgcttgt
    63061 ctacggccct gacggttgct caggtttgcg gcgtccagcg gttatgtaac tactattcgg
    63121 cgcgacttac gccgctcccc gggcctgatt cctccaggga aagcaatcat aggttggcac
    63181 aaatcacgca atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc
    63241 gccagcatct gaatgacgtc ggtcttactg ccggggactg tgtgatcatt aaccaaatca
    63301 ttggttttat tggctttcag gcgcggacca ttgcgacatt tcaggcttat ctcgggcacc
    63361 cggtacgctg gttacccggt ctggaaatac aaaactacgc cgacgcgtca ctgtttgctg
    63421 atgaatcaat acgctggcga agcagctatg aggtggaaaa actacctgaa gagtacacaa
    63481 aaagttcaac agcagaactt tgccaactgg ccgaaacact ctctctctcc accctatttc
    63541 actttccctt ctcgaaaggt tgttaaacag tacacgggtt aatacacagc cggataataa
    63601 gcttgcggcg ttgttatgcg cgcggataaa tggcagtcct gcttgttttg ccgcctgtat
    63661 ggattcatca aatgaatata aaaaaatcag cccccttctg cgcaagggcg aaaatgaaat
    63721 taaccaatgg gctgaccgtc attctgttga gcacgctacc gttcaggcga tacaatggct
    63781 gacccgagca cccgatcgct ttagcgccgc ccagttcagc cctttactcg aacacgaaaa
    63841 atcatcaacg cagattatta atctgctggt atggagcggg ctgtatggct ggataaatcg
    63901 cttaaaaatc gcgttgggtg agacatatta accttgccgc gtcagacaga ttcgcgtaaa
    63961 actgtcagcc gctctaatgg ccaccaaaat agacaattat gtttcaggac aacccgctgc
    64021 tagcgcagct taaacagcaa ctgcattccc agacgccacg cgctgaaggg gtggtaaaag
    64081 ccacagaaaa aggctttggc ttcctggaag tcgacgcgca aaaaagttat ttcattccgc
    64141 cgccgcagat gaaaaaagtc atgcatggcg accgaattat cgcggtgatc cacagtgaaa
    64201 aagaacgtga atccgcagag ccagaagaac tggttgaacc gtccctgact cgtttcgtgg
    64261 gtaaagttca gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag
    64321 acgccattcc ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg
    64381 cggttgccga aatgcgccgt catccgctga aaggcgatcg ttctttctat gctgaactga
    64441 cacaatacat cacttttggt gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc
    64501 ataatctgga aaaagaagca ccagacggcg tcgctaccgc aatgctcgat gaaggtctgg
    64561 ttcgtgaaga tctgaccgcg ctggattttg tcaccatcga cagtgccagc acagaagata
    64621 tggatgacgc ccttttcgct aaggcgttgc cggatgacaa acttcagctg attgtggcga
    64681 ttgccgatcc aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc gcgaaaattc
    64741 gcgcattcac caactatctg cctggcttca acatccctat gctgcctcgc gagctttctg
    64801 acgatctctg ctcactgcgc gccaatgaag tccgcccggt actggcatgc cgcatgacgc
    64861 tctccgctga tggcaccatt gaagataata tcgaattctt tgccgccacc atcgaatcca
    64921 aagcaaagct ggtgtatgac caggtttctg actggctgga aaataccggt gactggcagc
    64981 ctgaaagtga agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg
    65041 gcgagtggcg tcataaccac gcactggtgt ttaaagatcg cccggattac cgctttattc
    65101 tcggtgaaaa aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta
    65161 tcgtcgaaga agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc
    65221 tcggttttgg catctataac gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg
    65281 cagcgttgct gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc acgctggacg
    65341 gtttctgcaa actgcgtcgt gaactggacg cgcaaccaac tggtttcctc gacagccgca
    65401 ttcgtcgctt ccagtcattt gctgaaatta gcactgaacc cggtcctcac tttggcctcg
    65461 gtctggaagc atacgccacc tggacttcgc cgatccgtaa atatggcgac atgatcaacc
    65521 accgtctgct gaaagcggtt atcaaaggcg aaactgcgac gcgcccacag gatgaaatca
    65581 ctgtccaaat ggccgagcgt cgccgtctca accggatggc agaacgtgat gttggtgact
    65641 ggttatacgc acgcttcctg aaagacaaag ccgggaccga cacccgtttc gcagcggaaa
    65701 ttgtcgatat cagccgtggc ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct
    65761 ttattccggc acctttctta cacgctatgc gcgatgaact ggtttgcagc caggaaaacg
    65821 gcactgtaca aattaaaggt gaaacggttt acaaagtgac tgacgttatt gacgtcacta
    65881 ttgccgaagt ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg taatctcctt
    65941 tcacggccca ttcctcatgg atgggccgtt tatttccccg ctctaccttc gttatatcca
    66001 agcagatttt ttaactgact ttcgtttgaa aactggcgtt tttccaaaac tggattacaa
    66061 ataaatatat aaaaaaaaca atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt
    66121 tccttttatt taaccgcgga ctccgcagtt aaccggagga tatgcatcat gaaaaccgtt
    66181 agggagtcca caacgttgta caactttctc ggatcgcaca atccatactg gcggttgacg
    66241 gaaagcagcg atgttttgcg cttttctacc accgaaacca cagaacctga tcgtacattg
    66301 cagttatctg ccgaacaggc tgctcgcatc agggaaatga cggtcatcac ctccagcctg
    66361 atgatgagtc tgaccgtcga tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc
    66421 aataaacggg aatgggctgg caacgcgtct gcatggcatg acacaccggc ggttgctcgt
    66481 gatttatcac acgggctttc ctttgctgag caggtagttt ctgaagcaca ttccgcaata
    66541 gtgattctcg acagccgggg gaatatccaa cgcttcaatc ggttatgtga agattacaca
    66601 gggttgaaag aacacgacgt cattgggcaa agcgtgttta aactgtttat gagccgtcgt
    66661 gaagctgcgg catccaggcg caataaccgt gtattttttc gaagcggcaa tgcatatgaa
    66721 gtcgaactgt ggataccaac atgtaaaggc cagcggctgt ttctgtttcg caataaattt
    66781 gtccacagcg gcagtggcaa aaacgagatt tttttaatct gttccggcac cgacattacc
    66841 gaagagcgcc gcgctcagga gcgactgcgt attctggcaa ataccgacag tatcaccgga
    66901 ctgccgaatc gtaacgcaat gcaggattta atcgatcacg ctattaatca tgcagataac
    66961 aataaagttg gggttgtgta tcttgatttg gataatttca aaaaggtcaa cgacgcctat
    67021 gggcatttgt ttggtgacca gttattacgc gacgtgtcat tggctatttt aagctgtctc
    67081 gaacatgacc aggtgttggc gcgtccaggt ggggatgagt ttctggtact ggcatccaac
    67141 acctcacaaa gcgcgctgga agcaatggca tcacgaattt tgacccgctt acggctcccc
    67201 tttcgcattg gtttaattga agtttatacc agctgttcag taggtattgc actctctccc
    67261 gaacatggtt cagacagcac ggctattatt cgtcacgccg acacagcaat gtacacagcg
    67321 aaggaaggcg gacgaggaca attttgcgtt tttaccccag aaatgaatca acgggtattt
    67381 gaatatctct ggctggatac caacttgcgt aaagcactgg aaaacgatca gttggttatt
    67441 cactatcaac cgaaaatcac ctggcgtggc gaagtgcgca gtctggaagc actagtacgt
    67501 tggcagtcac ctgaacgtgg gttgattcca ccgttggact tcatttccta cgccgaagag
    67561 tcagggctaa ttgtgccttt aggccgttgg gtgattctcg atgtcgtacg ccaggtggca
    67621 aagtggcggg aaaaaggcat aaacctgcga gtggcggtaa atatttctgc acgtcagctc
    67681 gccgatcaaa ccattttcac cgccctgaaa caggttctcc aggaactcaa ttttgaatac
    67741 tgccctatag atgttgaact gacagagagt tgtctgattg agaatgatga actggcactg
    67801 tctgttattc aacaatttag ccaactaggt gcgcaagtgc atctggatga ttttggtacc
    67861 ggctactctt cactttcgca actggcgcgc tttccgatcg atgccatcaa acttgaccag
    67921 gtttttgttc gagatattca caaacaacct gtctcgcagt cactggtccg ggcgatcgtc
    67981 gctgtggccc aggcattgaa tcttcaggtg atcgccgaag gtgtagagag tgcaaaggaa
    68041 gatgcttttt taaccaagaa cgggatcaat gagcggcaag gatttttgtt tgccaaaccg
    68101 atgcccgccg tcgccttcga acgctggtat aaacgctatc tgaagcgcgc ataagtagat
    68161 gaatatatat acttagcgct atatcccgtc attatatatc gtattcctgg atgataatta
    68221 gccgttgctg ttaaacactt ctcttttagc ggcaattttt gcattattca ttttccccct
    68281 tttgctataa ggatgtcctt gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt
    68341 gatattgtgt tagatatcct tgtggcaggc gattaccact ctgcgatcca taatctcgag
    68401 atacttaaag cagaactctt gcgccaggtt gctgaatcta cgccagacat cccgaaggca
    68461 ccgtgggaga tttaacaatc gtgccttcag gttttcctat ttgaggatgc ccggcgcgtc
    68521 agtatttttt tatttagtat tataacgtta taagaattac agcgatgaat tcccgacaac
    68581 aaactattct acagatggtc attgaccagg gtcaggttag cgtaaccgat ctggcaaaag
    68641 ccactggagt ttctgaagtt accattcgcc aggatctcaa caccctcgaa aaactgagtt
    68701 acctccgccg tgcacatggc tttgcagttt cgcttgatag tgatgacgtc gaaacccgta
    68761 tgatgagcaa ctatacgctg aagcgtgaac tcgccgagtt tgccgcgtct ctggttcaac
    68821 cgggcgaaac catctttatc gaaaatggta gcagtaatgc cctgctcgcc cggactctgg
    68881 gcgaacagaa gaaaaatgtc actatcatca cagtcagcag ctacattgcg catttgctaa
    68941 aagacgcgcc ttgtgaagtc attttgctcg gtggcgtgta ccagaaaaaa agcgaaagta
    69001 tggttggccc tttgacacgc cagtgcatcc agcaggtaca tttcagcaaa gcatttattg
    69061 gtattgatgg ctggcaacct gaaactggat tcaccggtcg cgatatgatg cgtaccgatg
    69121 tggtcaatgc cgtgctggaa aaagagtgcg aagcgatagt cctgactgac agctcgaaat
    69181 ttggtgctgt acattcatac tccatcggtc ccgttgagcg attcaatcgc gtgattaccg
    69241 attcgaaaat acgcgccagc gatctgatgc atcttgagca cagtaaactc accgttcacg
    69301 tcgttgacat ttaaccattt cccttctcta cggatgattt gcagtttggc aaatcatccg
    69361 ctctaagatg attcctggtt gataattaag actatttacc tgttattaac acgcttaaga
    69421 tataaaatta ttatcagcga tataacagga agtcattatc acctgcgtga tataaccctg
    69481 cgcgcgagca gatttcacgg aataatttca ccaggcttat tcttagctat tatagttata
    69541 gagagcttac ttccgtgaat cataaattca ggagagagta ttatgtttgt aacgagcaaa
    69601 aaaatgaccg cggctgttct ggcaattact ttggcaatgt ctctgagtgc ctgttctaac
    69661 tggtctaaac gggaccgcaa caccgcaatc ggcgcgggtg caggggcatt aggcggtgca
    69721 gtactgaccg atggcagtac gttgggtaca ttaggtggtg cagctgtcgg tggtgttatt
    69781 ggtcaccagg tcggtaaata atcgtaaata aatgtcgatt atcgactcgc aattataata
    69841 cgcttgattc tgttcagtac gaaataactg aataatacac acagtaaaat attcgaaagc
    69901 cacggatata tccgtggctt tttatgttaa ccgcctgcga gttttacctt catccctttc
    69961 gcttcaagta gtgattttaa taaatcacgc ttatcgccct ggatttcaat aactccatct
    70021 ttaactgctc cgccgcagcc gcattttttc ttcagttccg ctgcaagttt tgtcagttcg
    70081 gcatcatcga gatcgacacc ggtaatcagg caaacgccct tacctttacg tccactggtc
    70141 tgacgctgaa tacgcaccac accgtcgcct ttagggcgta caggggccgc tttgggctca
    70201 tcaatacgtc cggtttccgt tgagtagacc agacggctgt tggaatcact catcatgcac
    70261 tccgctgtaa agaggcgttg atcgctttca gcgtctgcgc tggatctacc gattgcgtta
    70321 ccgggcgacc aatcaccata taatcaacac cagccgacaa cgcctgttct ggcgtcataa
    70381 tgcggcgctg gtcaccagct tcactcccct gcggacgaat gcccggcgta accagtttga
    70441 actcctgacc gaatacctgt ttaaagcgca cagcttcctg agcagaacac accacaccat
    70501 caaggccaca tttttgcgtc agtgccgcca gacgttctgc ataatctgca ggtgacagtg
    70561 tcatgccaag atcgaccagg tcgctggctt ccatgctggt caacactgtc acagcaatca
    70621 aaagcggtgc atctttgcca aacggaacca gtgcctcacg cgctgcggtc atcatacgcg
    70681 ccccaccaga ggcatgaaca ttcaccatcc acacgcctaa gtcagctgca gcagcgacag
    70741 cgtgcgcagc agtgttagga atatcgtgga atttcaggtc aagaaagata tcaaaaccac
    70801 gctgttgaag ttcgcgcaca aactgtggcc caaacaatgt aaacatctct ttgccgacct
    70861 tcagacgaca atcgcgtggg tcgatcttgt cgacaaagga cagcgcgtca tcacgattat
    70921 gataatcaag ggcaacaacc acaggagaat tcgtaacagc gcgggaagaa gatgaagcag
    70981 ttaacgtcat gaccagacct tcttgatgat gggcaccgga agtggcgcga aacaggtaaa
    71041 cggcgagcat tctacccgtg gaaataaaaa attaacagtt gcgatttttc ccctggcagg
    71101 ctttttctgc gccgtcgttg ccggagcaca atggaacatc aataactagt atgttgtaac
    71161 taaagtagga ttttttaaaa attacaggcc atcaagaccg cgaatcggtt taatggttga
    71221 ccaggcccga caagacggac aatgccagta gagggtgtat gcggtaaaac cacatttctg
    71281 gcagcgataa cgaggcttac tacgtacctt ttcgccaacc atgtcacgca gcaccatcag
    71341 gctctctttg gcacgccctt cttccgcttc atttaagtgg taatccatta acttatggaa
    71401 cacacgcatg gtcggatgac gctgaagctg gcgcgtaata tagacctgtg cggcctcact
    71461 accgtcgcgc gcttcgatga tatcagcaag catcaattca gcatcggcac cggtgttctc
    71521 ttccaccgcg cgttgcagga attctgccca ttcggcagtt ttacccaact gctgatagca
    71581 ggtttgcaac atttccagcg tttcgctgac cagttctctg tcctgggata tcacgcgttg
    71641 caaactttcg acggctttgg cgtattctcc tttcgccata aacacgcgtc ccatcatgat
    71701 ggatacgcgg gcgctgtttt tatctgccgc cgcccctttt tttagcaagg tcatggcacg
    71761 atcgagatcg tcgctggcca tatgctgcag ggctaactca cagtagaaat gggcaatttc
    71821 gacgcgctgt ttatctttac ccagcttcac caggcgttcg gcaacatcaa ttgctttctg
    71881 ccactcactg gtagcctggt agatttgtag caactgttgc agagcgccaa tgcggaagtc
    71941 agtttcatcg gtcagctgat tgaacatgtc ttccgcgcgg tcgtataacc cggcggccat
    72001 gtaatcacgc cctagttgtt gaatcgccaa cagacgctgt tcataggtca gcgaggcgct
    72061 ttccattagg gtctgatgga tgcgaatagc gcgatcaact tcgccacgcg aacggaacag
    72121 gtttccgagc gtaaggtggg cttcaacggt gcctgtatcc tctttaagca tatcgagaaa
    72181 caggtctacc gctttatcct gttgattact aagcaggaag ttaacccccg ctacgtaatc
    72241 acgcgacaag cggttggctt catcttgctt gttttgttgc gcacttctgc ggcccatata
    72301 ccagccatag gcagcggcta caggcaaaag cagaaacaac aactccagca tagaaagtta
    72361 ttccttcgcc gctgacgagt gcggcactac agccacgtca gtcgcgggtg aaagctggtt
    72421 ttccagtcgc tttattttac gttcagcgcg cgccagggaa acacgaactc gcagccagaa
    72481 caggccacaa atcaaccaac cgatagcaaa ccccgcagca aacaataccg ccagcaatgt
    72541 ggaaatacgg tactcccctt gcgctaacag ataattaaac gtcacctgtt gatcgttctg
    72601 cgcacccaac gtgaccgaaa tcacgaagat cgctaacacc agtaagaaaa tgagtaaata
    72661 tttcacatta cttcccgtta tgtggttaga gcgaataaat tatggtcaac ttaccgcaat
    72721 agccctttaa agtaccattt tcgcgccgtc tgcgaaatgg catccacgcg cattgttgat
    72781 gatttaaggg ctaaattcgg aaaatcaacc agcgttaact ttcttgttct cgttgcgcta
    72841 tttcgcgatt ttcttccgca ggtggtgtta atggcccaca aattcgttgc gcaagccacg
    72901 ttgccaccgc caccagcgcc cacgaaatca acgtagctac taccagatcg cgtggccaat
    72961 gcatcccgag cagcaggcgg cttcccatga ctcccgttgc ccagaccagc aagatagcaa
    73021 tggttaacgt tcgccgacgc ggccacagca aaccaacggc cagcagtgcc caactggcag
    73081 caaacatcgt gtgaccggaa ggaaaggcaa accccgtctc tttctgccaa tgtgaacgca
    73141 aatattgtgg gatatttttc tcttcagcca actgttcttt cactagattt ccgcgttctg
    73201 ctcgctttaa agtgtagaac tcatcaaccg gaatatgatg tgttttttcc agccagataa
    73261 caaaaggtcg tggttcctgg actttgtctt tgatccagga tttaacgcct tgtcccacaa
    73321 ggattgcggc cgccagaatg gcaaataata caaaggcagc cttaatgcga aaacgcagac
    73381 accagagaaa ccagccgaat aaaatcaaat gtgtaatgac gccccagggc tgggtgacag
    73441 tttcagtaac ccaaaaagcc gcttttagta gccaactttg ttctccaggt tgccaacgcc
    73501 agccagaaat ccatacggct actggcatga caagcaatag tgcagctccc actgcggtac
    73561 gtctggcaat cgaacgcatg gcctctcctt tttgataagt cccacaatca taactgaaaa
    73621 cgccagttcc aggaaaaatt gacagatttg tgccattccg tgaacgatcg acgcgtcgtg
    73681 attaggtgaa ccccttctcg ttatggcaaa ataagccaat acagaaccag cattatctgg
    73741 agaatttcat gcagcttaaa cgtgtggcag aagccaaact gccaacccca tggggcgatt
    73801 tcctgatggt gggatttgaa gaactggcaa ccggacacga tcatgtcgcg ctagtctatg
    73861 gcgatatttc cgggcatacc ccggtacttg cgcgcgtcca ttccgaatgt ctgaccggtg
    73921 acgccctgtt cagcttgcgc tgcgattgtg gcttccagct cgaagcggca ttgacgcaaa
    73981 ttgccgagga aggccgtggt attttgctgt atcaccgtca ggaaggtcgt aacattggtc
    74041 tgctgaataa aatccgcgct tacgcactgc aggatcaagg ttacgatacc gtagaggcta
    74101 accaccagtt aggcttcgcc gctgatgagc gcgacttcac tctttgcgct gatatgttca
    74161 aactccttgg cgtcaatgaa gtccgcttgt taaccaataa cccgaaaaaa gtcgaaattc
    74221 tgaccgaagc agggattaat attgttgaac gcgtaccatt gattgtaggt cgtaacccca
    74281 ataacgaaca ttatctcgat accaaagccg agaaaatggg ccatttgctg aacaaataac
    74341 cctcttgcat tgtgtaatta atttgcttgc cggaagcaaa ataaccggca agcaaatact
    74401 tgttacttca acatattacg aatgacataa tgcaaaatgc cgtcgttctg gtagtaggtc
    74461 aactccgtcg cggtgtcgat acgacaacgg cagggtacga cctcctggct accatccgcg
    74521 cgcgtaagcg tcaccggaac cgtcgcaccg ggttgtagat tttgcagatc gccaatatca
    74581 atcttctctt ccccggttag tcctaacgtt ttacgcgtta cgccttgcgg aaattccagc
    74641 ggcaggatgc ccatgccaat taaattcgaa cggtgaattc gttcaaacga ttcggcaatc
    74701 accacacgaa ttccaagcag acgaggacct tttgccgccc agtcacggct tgagcctgat
    74761 ccatactctt tcccggcaat caccgccagc ggcgtttgtt ccttcttata gcgcatcgca
    74821 gcatcataaa tagagactac gtcgctgtca ggtaaatgcc gcgtcatccc cccttcaacg
    74881 ccaggcacca tttcattacg gatgcgaata ttggcgaagg tgccgcgcat catcacttca
    74941 tggttaccac gccgcgaacc gtaggagtta aagtcttttc gctcaacacc ccgaccttgt
    75001 agatatcgac ccgctgggct gtcgggctta atactgcccg ccggagagat atggtcagtg
    75061 gtgactgaat cccccagcat tgcgaggatc cgcgcaccgt gaatatcttc cactggtgct
    75121 ggtgttgcct gcatttcatc aaagaaaggc gataagcgaa tataggttga gtcctcctgc
    75181 caaccgtagg tatcggatcg tgtgacgtta atttccttcc actctgctgt gccttcaaaa
    75241 acttctgcgt actctttgcg gaacatttct gtggagactt gttctaccgc acgggcaatt
    75301 tcttgtgccg atggccagat atctttcaga taaaccgggt cgcctttacg atcatgacca
    75361 ataggctcag cagccaggtt gatattcata tttcccgcca gcgcataggc aaccaccagc
    75421 ggcggcgagg ccagccagtt agttttaacc agcggatgga tacggccttc aaagttacgg
    75481 ttgccggaca gcaccgcacc gacggttaaa tcgccttttt tgattgccgt ttcgatagga
    75541 tcgggcagcg gcccagagtt accaatacag gtggtacaac cgtatcccac aaggttaaac
    75601 cccagttcgt cgagatacgg tgtcagtttc gcttttgcca gataatcaga aacgactttc
    75661 gaacccggtg ccagcgacgc tttgacccac ggttgccgct tgaggcccag agttacggct
    75721 tttttcgcca gcaagcctgc ggccatcagc acacttgggt tagaggtgtt ggtgcacgag
    75781 gttatcgcag caatgaccac agcgccatca ggtaactgat actgatgtcc gttcataaca
    75841 taatcgaccg gctggcgatc tttatgcgtg gcattcactt ccagttcgtt actggcggca
    75901 aatgcttttg gtacatcggg cagtgcaacg cgatcctgtg ggcgttttgg ccctgccagg
    75961 ctcgcttcaa cgtcattcat atccagttct aacgtactgg taaaaatcgg ttcatcgccc
    76021 gggttacgcc acatgccctg cgctttggca tatttttcga ccaactcaac ctgatcttcg
    76081 ctgcgcccgc ttaaacgcat gtaatcgagg gttacagcat cgattgggaa gaagccacag
    76141 gtggcaccat attctggcga catattggca atggtggcgc gatccgccag cggtagtgaa
    76201 tccagaccat caccataaaa ttcgacgaat ttccccacca cgccatgttt gcgcagcatt
    76261 tgggtaacag tgagaaccag gtctgtggcg gtaatacctt cacgtaattt accggtaagt
    76321 ttaaagccca ctacgtccgg gataagcatg gaaaccggct ggcctaacat tgcggctt
//
LOCUS       DAZFKM010000006        73566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFKM010000006 DAZFKM010000000
VERSION     DAZFKM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHKAY_000454"
     CDS             complement(65..1309)
                     /gene="mtr"
                     /locus_tag="ACHKAY_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO5699187.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHKAY_000455"
     CDS             complement(1463..3352)
                     /gene="deaD"
                     /locus_tag="ACHKAY_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO5699188.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHKAY_000456"
     CDS             complement(3345..3425)
                     /gene="yrbN"
                     /locus_tag="ACHKAY_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO5699189.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHKAY_000457"
     CDS             complement(3532..4416)
                     /gene="nlpI"
                     /locus_tag="ACHKAY_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO5699190.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHKAY_000458"
     CDS             complement(4525..6660)
                     /gene="pnp"
                     /locus_tag="ACHKAY_000458"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO5699191.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHKAY_000459"
     CDS             complement(6907..7176)
                     /gene="rpsO"
                     /locus_tag="ACHKAY_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO5699192.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHKAY_000460"
     CDS             complement(7325..8269)
                     /gene="truB"
                     /locus_tag="ACHKAY_000460"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO5699193.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHKAY_000461"
     CDS             complement(8269..8670)
                     /gene="rbfA"
                     /locus_tag="ACHKAY_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO5699194.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHKAY_000462"
     CDS             complement(8834..11506)
                     /gene="infB"
                     /locus_tag="ACHKAY_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO5699195.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHKAY_000463"
     CDS             complement(11531..13018)
                     /gene="nusA"
                     /locus_tag="ACHKAY_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO5699196.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHKAY_000464"
     CDS             complement(13046..13498)
                     /gene="rimP"
                     /locus_tag="ACHKAY_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO5699197.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13705..13781)
                     /locus_tag="ACHKAY_000465"
     tRNA            complement(13705..13781)
                     /locus_tag="ACHKAY_000465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13745..13747),aa:Met,seq:cat)
     gene            14129..15472
                     /gene="argG"
                     /locus_tag="ACHKAY_000466"
     CDS             14129..15472
                     /gene="argG"
                     /locus_tag="ACHKAY_000466"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO5699198.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHKAY_000467"
     CDS             complement(15480..17105)
                     /gene="yhbX"
                     /locus_tag="ACHKAY_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5699199.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17564..17650)
                     /locus_tag="ACHKAY_000468"
     tRNA            complement(17564..17650)
                     /locus_tag="ACHKAY_000468"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17614..17616),aa:Leu,seq:gag)
     gene            complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHKAY_000469"
     CDS             complement(17665..17997)
                     /gene="secG"
                     /locus_tag="ACHKAY_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO5699200.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHKAY_000470"
     CDS             complement(18225..19562)
                     /gene="glmM"
                     /locus_tag="ACHKAY_000470"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO5699201.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHKAY_000471"
     CDS             complement(19555..20403)
                     /gene="folP"
                     /locus_tag="ACHKAY_000471"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO5699202.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHKAY_000472"
     CDS             complement(20493..22427)
                     /gene="ftsH"
                     /locus_tag="ACHKAY_000472"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO5699203.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHKAY_000473"
     CDS             complement(22527..23156)
                     /gene="rlmE"
                     /locus_tag="ACHKAY_000473"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO5699204.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHKAY_000474"
     CDS             23282..23575
                     /gene="yhbY"
                     /locus_tag="ACHKAY_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO5699205.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHKAY_000475"
     CDS             complement(23731..24207)
                     /gene="greA"
                     /locus_tag="ACHKAY_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO5699206.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24455..25888
                     /gene="dacB"
                     /locus_tag="ACHKAY_000476"
     CDS             24455..25888
                     /gene="dacB"
                     /locus_tag="ACHKAY_000476"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5699207.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHKAY_000477"
     CDS             complement(25928..27100)
                     /gene="cgtA"
                     /locus_tag="ACHKAY_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO5699208.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHKAY_000478"
     CDS             complement(27116..28081)
                     /gene="yhbE"
                     /locus_tag="ACHKAY_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5699209.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHKAY_000479"
     CDS             complement(28208..28465)
                     /gene="rpmA"
                     /locus_tag="ACHKAY_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO5699210.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHKAY_000480"
     CDS             complement(28486..28797)
                     /gene="rplU"
                     /locus_tag="ACHKAY_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO5699211.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29056..30027
                     /gene="ispB"
                     /locus_tag="ACHKAY_000481"
     CDS             29056..30027
                     /gene="ispB"
                     /locus_tag="ACHKAY_000481"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO5699212.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHKAY_000482"
     CDS             30254..30532
                     /gene="sfsB"
                     /locus_tag="ACHKAY_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO5699213.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHKAY_000483"
     CDS             complement(30580..31839)
                     /gene="murA"
                     /locus_tag="ACHKAY_000483"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO5699214.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHKAY_000484"
     CDS             complement(31894..32148)
                     /gene="ibaG"
                     /locus_tag="ACHKAY_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO5699215.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHKAY_000485"
     CDS             complement(32308..32601)
                     /gene="mlaB"
                     /locus_tag="ACHKAY_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO5699216.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHKAY_000486"
     CDS             complement(32601..33236)
                     /gene="mlaC"
                     /locus_tag="ACHKAY_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO5699217.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHKAY_000487"
     CDS             complement(33255..33806)
                     /gene="mlaD"
                     /locus_tag="ACHKAY_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO5699218.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHKAY_000488"
     CDS             complement(33811..34593)
                     /gene="mlaE"
                     /locus_tag="ACHKAY_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO5699219.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHKAY_000489"
     CDS             complement(34601..35410)
                     /gene="mlaF"
                     /locus_tag="ACHKAY_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO5699220.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHKAY_000490"
     CDS             35620..36597
                     /gene="yrbG"
                     /locus_tag="ACHKAY_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO5699221.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHKAY_000491"
     CDS             36611..37597
                     /gene="kdsD"
                     /locus_tag="ACHKAY_000491"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO5699222.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHKAY_000492"
     CDS             37618..38184
                     /gene="kdsC"
                     /locus_tag="ACHKAY_000492"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO5699223.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38181..38756
                     /gene="lptC"
                     /locus_tag="ACHKAY_000493"
     CDS             38181..38756
                     /gene="lptC"
                     /locus_tag="ACHKAY_000493"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO5699224.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38725..39282
                     /gene="lptA"
                     /locus_tag="ACHKAY_000494"
     CDS             38725..39282
                     /gene="lptA"
                     /locus_tag="ACHKAY_000494"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO5699225.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39289..40014
                     /gene="lptB"
                     /locus_tag="ACHKAY_000495"
     CDS             39289..40014
                     /gene="lptB"
                     /locus_tag="ACHKAY_000495"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO5699226.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHKAY_000496"
     CDS             40062..41495
                     /gene="rpoN"
                     /locus_tag="ACHKAY_000496"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO5699227.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41518..41805
                     /gene="hpf"
                     /locus_tag="ACHKAY_000497"
     CDS             41518..41805
                     /gene="hpf"
                     /locus_tag="ACHKAY_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO5699228.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHKAY_000498"
     CDS             41923..42414
                     /gene="ptsN"
                     /locus_tag="ACHKAY_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO5699229.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHKAY_000499"
     CDS             42460..43314
                     /gene="rapZ"
                     /locus_tag="ACHKAY_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO5699230.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43311..43583
                     /gene="npr"
                     /locus_tag="ACHKAY_000500"
     CDS             43311..43583
                     /gene="npr"
                     /locus_tag="ACHKAY_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO5699231.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHKAY_000501"
     CDS             43797..44429
                     /gene="yrbL"
                     /locus_tag="ACHKAY_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO5699232.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHKAY_000502"
     CDS             complement(44426..45154)
                     /gene="mtgA"
                     /locus_tag="ACHKAY_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO5699233.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHKAY_000503"
     CDS             complement(45151..45804)
                     /gene="elbB"
                     /locus_tag="ACHKAY_000503"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO5699234.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHKAY_000504"
     CDS             complement(46034..48370)
                     /gene="arcB"
                     /locus_tag="ACHKAY_000504"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO5699235.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHKAY_000505"
     CDS             complement(48466..49395)
                     /gene="yhcC"
                     /locus_tag="ACHKAY_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO5699236.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSITEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50070..54530
                     /gene="gltB"
                     /locus_tag="ACHKAY_000506"
     CDS             50070..54530
                     /gene="gltB"
                     /locus_tag="ACHKAY_000506"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO5699237.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54543..55961
                     /gene="gltD"
                     /locus_tag="ACHKAY_000507"
     CDS             54543..55961
                     /gene="gltD"
                     /locus_tag="ACHKAY_000507"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO5699238.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHKAY_000508"
                     /pseudo
     CDS             56145..57192
                     /gene="yhcG"
                     /locus_tag="ACHKAY_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHKAY_000509"
     CDS             complement(57252..57716)
                     /gene="nanQ"
                     /locus_tag="ACHKAY_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO5699239.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHKAY_000510"
     CDS             complement(57713..58588)
                     /gene="nanK"
                     /locus_tag="ACHKAY_000510"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO5699240.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHKAY_000511"
     CDS             complement(58585..59274)
                     /gene="nanE"
                     /locus_tag="ACHKAY_000511"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO5699241.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHKAY_000512"
     CDS             complement(59322..60812)
                     /gene="nanT"
                     /locus_tag="ACHKAY_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO5699242.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHKAY_000513"
     CDS             complement(60922..61815)
                     /gene="nanA"
                     /locus_tag="ACHKAY_000513"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO5699243.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHKAY_000514"
     CDS             complement(61937..62728)
                     /gene="nanR"
                     /locus_tag="ACHKAY_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO5699244.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            63108..64475
                     /gene="dcuC"
                     /locus_tag="ACHKAY_000515"
     CDS             63108..64475
                     /gene="dcuC"
                     /locus_tag="ACHKAY_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5699245.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(64518..65015)
                     /gene="sspB"
                     /locus_tag="ACHKAY_000516"
     CDS             complement(64518..65015)
                     /gene="sspB"
                     /locus_tag="ACHKAY_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO5699246.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(65021..65659)
                     /gene="sspA"
                     /locus_tag="ACHKAY_000517"
     CDS             complement(65021..65659)
                     /gene="sspA"
                     /locus_tag="ACHKAY_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO5699247.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(66054..66446)
                     /gene="rpsI"
                     /locus_tag="ACHKAY_000518"
     CDS             complement(66054..66446)
                     /gene="rpsI"
                     /locus_tag="ACHKAY_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO5699248.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(66462..66890)
                     /gene="rplM"
                     /locus_tag="ACHKAY_000519"
     CDS             complement(66462..66890)
                     /gene="rplM"
                     /locus_tag="ACHKAY_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO5699249.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(67109..68236)
                     /gene="zapE"
                     /locus_tag="ACHKAY_000520"
     CDS             complement(67109..68236)
                     /gene="zapE"
                     /locus_tag="ACHKAY_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO5699250.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            68430..68828
                     /gene="zapG"
                     /locus_tag="ACHKAY_000521"
     CDS             68430..68828
                     /gene="zapG"
                     /locus_tag="ACHKAY_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO5699251.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            68982..70349
                     /gene="degQ"
                     /locus_tag="ACHKAY_000522"
     CDS             68982..70349
                     /gene="degQ"
                     /locus_tag="ACHKAY_000522"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO5699252.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            70439..71506
                     /gene="degS"
                     /locus_tag="ACHKAY_000523"
     CDS             70439..71506
                     /gene="degS"
                     /locus_tag="ACHKAY_000523"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO5699253.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(71569..72507)
                     /gene="mdh"
                     /locus_tag="ACHKAY_000524"
     CDS             complement(71569..72507)
                     /gene="mdh"
                     /locus_tag="ACHKAY_000524"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5699254.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            72942..73412
                     /gene="argR"
                     /locus_tag="ACHKAY_000525"
     CDS             72942..73412
                     /gene="argR"
                     /locus_tag="ACHKAY_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO5699255.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        18035 a        19790 c        18169 g        17572 t
ORIGIN      
        1 cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga taaggcaccg
       61 ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc agtgcgttgc
      121 caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg cggaatttcg
      181 ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt gccgcccaga
      241 tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg aacagtagac
      301 cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc agggctgagt
      361 catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg ccaaggaacg
      421 aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta cggctgttca
      481 gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct gcaataccga
      541 taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta tacagcgcca
      601 gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct ttgccgtaat
      661 acttcatcag gcttggcacg ttaccgtgat aaccaaacga cgccaggcag aacggcaatg
      721 tcatcagcag atacggcgca taagatgcat tgctttctgc aacgttgaac aatgtcgcag
      781 gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa ggtaatgact ttcgccccca
      841 gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa ccacaccaca aacgctacca
      901 gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt tagcgacatc tctgcgaagg
      961 tgtgatgcag aattgaacca ctggcagaaa tataggcata ggtcaggata tagagcacaa
     1021 aggcaatgga aataccgttg accacgttcc agcctttgcc cagcaaatct ttggtgatgg
     1081 tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc aagccggaat
     1141 gcagcataca gaaccaggta aagatcagcg ccgccattga ccagaaaaac cacgccccgg
     1201 acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg ataatcacca
     1261 cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg agggcttctc
     1321 tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca ctgtaccagt acacgagtac
     1381 aaaagacaga aaaaaagccc cgatggtaaa aatcggggct gtatatatta ttttacagat
     1441 tgtgttcgct gttcagcgat gattacgcat caccaccgaa acgacgacga ccggtagagt
     1501 catcacgacg cggagcgcgg ccttcacgac gttcgccgct aaaacgacga ccatcaccac
     1561 ggccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgt tcgccaccga
     1621 aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg cccagcaact
     1681 gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt acttcacccg
     1741 gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg ttaccgatgt
     1801 aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga acttcaacac
     1861 catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca cgacgttcac
     1921 gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg cgcggaccac
     1981 ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc ggaacaatca
     2041 gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg agatcctgct
     2101 cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga tccagatcgc
     2161 tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt ttgcccagca
     2221 gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt tcaatgttgc
     2281 gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca cgacccgcac
     2341 gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata tcgtagttaa
     2401 ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc gcaatcagga
     2461 tgtccagacg accatctttc aggcgttcca gtgtctgttc acgcagcgcc tggttcatgt
     2521 caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc acttccagag
     2581 tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct tccaggaaac
     2641 gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg ctgatgtcag
     2701 gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata aagcggcggg
     2761 taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga tgaccttccg
     2821 ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc atttcgtcag
     2881 cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt ttcaggtggt
     2941 ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg gcgcgtaatt
     3001 gcacgtcata acgctggccg ccgtatagag caaccacatt tacgccgcgc atgtgtttag
     3061 agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt gccagcacca
     3121 gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa gagaatgctg
     3181 cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc agcagatgtg
     3241 gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta agggcttcaa
     3301 ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc atgtagtacg
     3361 tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg atcagcaatt
     3421 ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca acgccctcac
     3481 ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca gctattgctg
     3541 gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca acaatgcgta
     3601 tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca gtgccgtggc
     3661 gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg tttcactgag
     3721 atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca ttaacgtttg
     3781 ttcgctaatg ttgcccaggt agaactcgac aatgttccat ccccactgtt ccttatccga
     3841 tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct tctgctcggc
     3901 gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa acgccagcag
     3961 atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc gattcaagtg
     4021 cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat aggcagcatc
     4081 aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag gcatatccgg
     4141 tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac cgagactatc
     4201 atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta aagcccgact
     4261 ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg gttgcaatgg
     4321 taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa gcgtaagtgc
     4381 tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa gaccacggtt
     4441 gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa agccccccgc
     4501 cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag cttccggtgc
     4561 tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac ggccctggcg
     4621 atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat cggtcacttt
     4681 ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac cgccgccgat
     4741 ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga cgcggcccac
     4801 ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt tctcgccgtc
     4861 ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc cagtttcttc
     4921 ggtcagggca cggataacag agccgccttt accgataacg tctttgatct tgtccgggtt
     4981 gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg gcgcgttgat
     5041 cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt tcagcgcaac
     5101 ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcaatg cagagatacc
     5161 ctcgcgggaa cccgcaactt tgaagtccat atcgcccagg tgatcttcgt cgcccaaaat
     5221 gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga tacccgcaac
     5281 ggcagctttg atcggcacac ctgcgtccat cagcgccaga gacgcgccgc acacggaagc
     5341 catagaagag gaaccgttgg attcagtgat ttcagacaca acacgtacgg tgtacgggaa
     5401 tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac cgtgaccaat
     5461 ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg gagggaagtt
     5521 gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa cctgcgcgtc
     5581 acgagcagta cccagcgttg cggtaaccag cgcctgcgtt tcaccacggg tgaacagcgc
     5641 agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga tcatatcttt
     5701 ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa cgtttttctc
     5761 gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg gtttcgtctt cagcaagcag
     5821 cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt gtttgtcggt
     5881 gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg cgttcagcgc
     5941 ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt tcaccagttc
     6001 attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg cgcccagcat
     6061 ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg cttcagtacc
     6121 ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt tcagtacgta
     6181 ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg gaataccaga
     6241 cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa cttgcgggtt
     6301 aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg ggaacagcgg
     6361 gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg ggcggccttc
     6421 acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct gatagttaac
     6481 ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa cggtaacaaa
     6541 taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag ccatcatgcc
     6601 ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg gattaagcaa
     6661 tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa tacccgatct
     6721 tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt aaagcctctc
     6781 attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca ttagtttcca
     6841 gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt ctgaaactcg
     6901 caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg ctacgtcttt
     6961 acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca gaccacgacg
     7021 gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga tctgtgcagt
     7081 cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac caaactcaga
     7141 aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa gtatatagaa
     7201 tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa gtgttaaaca
     7261 atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg caagacggtt
     7321 aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct tcatcgtcaa
     7381 tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc agtccttcca
     7441 gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa gacgttaacg
     7501 gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt aatggatcaa
     7561 gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca cgcagatgtt
     7621 ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc aggtaaataa
     7681 catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat gatggtgcgg atataagtgc
     7741 cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata aacagcaatt
     7801 cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc gcatattcgt
     7861 acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt tcgatatcgc
     7921 cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc ggacgttctt
     7981 caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc gcaatgaccc
     8041 gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc ccgaggcaaa
     8101 ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca cggttggcgt
     8161 tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc gatttatcca
     8221 gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt agtcctcctt
     8281 gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg tcaccaggtt
     8341 tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt ccggcacgat
     8401 acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt cttgcaacgc
     8461 tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca catatacttt
     8521 ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca tgcccaggcg
     8581 aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt cctgcgctac
     8641 gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa aaggggctgt
     8701 tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc tgataagacg
     8761 cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta tcccgcatgg
     8821 aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat acttcgatca
     8881 catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc ataccgttac
     8941 ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct tcgtagataa
     9001 ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct tcggtaacca
     9061 tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact tccgccagac
     9121 cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct ttcacttcgt
     9181 caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct tcaatcactt
     9241 tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg gacgccgcag
     9301 ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc ttcactttaa
     9361 cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa ccctgtacgt
     9421 ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg gcgaacatgt
     9481 tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta ccctgacgat
     9541 acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca tcacccgcag
     9601 ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct tccagcactt
     9661 cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca cacagaacga
     9721 tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga ccacgacctt
     9781 tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc gctttcagct
     9841 ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg gtacccgctt
     9901 tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg atgccatact
     9961 gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc ttgttcactg
    10021 caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg gtctgcggca
    10081 tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc gcaccacgtg
    10141 cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc atgccgtttt
    10201 cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg ccagaggcaa
    10261 ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg tgacccatga
    10321 tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg ctcattaccg
    10381 cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct tcagcaacca
    10441 gttgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc agtttcatca
    10501 tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg ccaacggtga
    10561 tggtttcgcc gatcacaacg tcacggttaa cggcctgagc tggcttctgg aagccttgct
    10621 gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg cgtgcttctt
    10681 cacgatcagc ttttgattca gcgtgtttgt tgcctttctt cggacgcgct gctttcgcgt
    10741 tacgaccacg gccacgaccg ccttcgactt cacgatcgct ttcgtcttct gcctggcgag
    10801 catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc gcgttatcag
    10861 tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt gcttcttctt
    10921 cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct tcctgctcac
    10981 ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg ctcactttgt
    11041 ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct tgttctgcgg
    11101 cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc tcacgacggg
    11161 cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca gcctcttgcg
    11221 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    11281 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    11341 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    11401 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    11461 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    11521 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    11581 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    11641 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    11701 tcccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    11761 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    11821 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    11881 tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    11941 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    12001 tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    12061 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    12121 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    12181 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    12241 gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt ttatcgttgg
    12301 ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta atttcaatca
    12361 cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    12421 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag agcacgccac
    12481 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    12541 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    12601 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    12661 cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg tcaaaggtaa
    12721 cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct tcataacgtg
    12781 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    12841 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg atctcttgtt
    12901 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    12961 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    13021 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    13081 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    13141 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    13201 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    13261 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    13321 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    13381 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc agttcaaaac
    13441 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    13501 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    13561 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    13621 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    13681 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    13741 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    13801 tactcgtttt gggtaaaaga tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    13861 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaataac
    13921 gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc ctgctctccc
    13981 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    14041 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    14101 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    14161 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    14221 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc cagacgaaga
    14281 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    14341 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    14401 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    14461 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    14521 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    14581 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    14641 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    14701 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    14761 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    14821 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtgg cgctgaacgg
    14881 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    14941 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    15001 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    15061 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    15121 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    15181 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    15241 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    15301 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    15361 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tctcctcctc
    15421 tgccacttca ggcgtgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    15481 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    15541 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    15601 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    15661 ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt ccgttgtgcg atccacgcca
    15721 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    15781 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    15841 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    15901 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    15961 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    16021 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    16081 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    16141 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    16201 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    16261 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    16321 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    16381 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    16441 atacctgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    16501 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    16561 gcaaatcgcg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    16621 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    16681 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    16741 aaaaaagcaa aggcacatag ataagggata tacatcccca gcattttgac aacttcatcc
    16801 ggatcactct ggagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    16861 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    16921 agcaggcgaa gcggaaggga tgatatacgt tttacaacca ggatggtcag gacgaagaac
    16981 agcaatcgct gaaccatatg cgcgccggaa gaggcgacta agttcgtaat accaaaaaca
    17041 ataacacaaa gatacaacaa ccaatgagat ttaaaacttc tagcaaattt gttgaatact
    17101 gtcatccaga aatccaagtt cacaaccaat ataaacaatt atcaacaatg ttcaatttta
    17161 tatcagacat gtttttcaac tctgatccat atcagaccga aacactgtta gttttttctt
    17221 tttaaatgat gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa
    17281 acattacgca gaacgtagcc atcagtagtt atactgacag gagcaggatg gtttatcgag
    17341 aagtaaacat atccgtattc gtaccggaac tggagttgac acaataaagt gccaattatg
    17401 tcagcagaag ggaaaagttt ataactaagg cgtaccggca ccatcgtttc aaggtaccag
    17461 ctacgagtaa agcaactgga cgagatacag atacctgaca accattcctc agaccaggac
    17521 caaaacgaaa aaagacgctt ttcagcgtct cttttctgga atttggtacc gaggacggga
    17581 cttgaacccg taagccctat tgggcactac cacctcaagg tagcgtgtct accaattcca
    17641 ccacctcggc acggatacta ctttttagtt cgggatatcg ctggtcggct tagccggagc
    17701 agctggttga gtttgttcgg ttttcaccgg tgcactcaga ttttcccatt cgctaccttt
    17761 attggttttg ttgctattga tgttacccag caccagactg atgatgaaga ataacgttgc
    17821 cagcagcgcc gtcatgcggg tcatgaagtt accagaacca cttgaaccaa acagcgtagc
    17881 ggaagcgcct gctccgaagg aggctcccat atcagcgcct ttaccttgct gcagcatgat
    17941 cagaccaaca aggccaattg ccacaataag gaaaactact aaaagagctt cgtacataat
    18001 caacctgttc cttgcggagt tgccgcgtac caatgcttca accaataaag cgggagtttt
    18061 taatctttcc cactgaagcg ggtgtgaata ctaaccaaag cgaatgacct tcgcaagggc
    18121 aattttatcg cattgtatca actgcggaaa aaaacagcaa aaccgatgtg ttcgctgaga
    18181 aaaaaggcga caatcgccgc ctttttagcc agttatctaa cgctttaaac ggcttttact
    18241 gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt cgtcttcgcc ttccaccatc
    18301 acgcgaatta acggttcggt gccggattta cgcagcaaca cgcgtccacg gttgcccagc
    18361 gcagcttcaa cctctgcggt cacggcttta actgactcat gctcaagtgg atcgccgcta
    18421 cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca ttttcatgcc gctgcaaagg
    18481 tcgtgcaggc tcatatggtt acgtgccatc gccgccagca cctgcaagcc agcaacgatg
    18541 ccgtcaccgg tagtagtttt atccagtagg atcacatgac cggaattctc tgcaccgata
    18601 cgccagcctt tctcctgcat tttttccagt acgtagcggt cgcccacttt cgcgcgcgca
    18661 aatggaattc ctaactgttt cagcgccagt tcaagcccca tgttgctcat caatgtaccc
    18721 acagcgccac cacgcagctg gccctgacga agaccttcac gcgcgatgat atacatgatc
    18781 tgatcgccat cgactttatt gccttcatgg tcaaccataa tcacgcgatc gccatcgccg
    18841 tcgaaggcaa taccgagatc cgctttttca gccagcacac gagcctggag cgcgcgaacg
    18901 tcggtagccc ccacttcggc attgatgttt acaccgtttg gctcacaacc gatagcgata
    18961 acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt gataagtcgc accgtttgca
    19021 caatccacca caatcttcag ttcactgagg ctaagttcgt tcgggaacgt ggctttgcaa
    19081 aactcgatat agcgacccgc ggcatcaacg atacggctgg ctttacccag ttctgccgaa
    19141 tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg cctcttctac cgcatccggc
    19201 agtttggtgc cgtcgataga gaagaattta atgccattat cgtagaacgg gttatgagat
    19261 gcagatatca caattccggc ctctgcgcgg aaggtacgcg tcagataagc cacggccggt
    19321 gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg ccgccagacc cgcttccagt
    19381 gctgactcca gcatatagcc agaaatacgc gtgtctttac caataataat cttacgggag
    19441 ccgtggcgcg ccagcacttt acccgcggcc caacccagct taagcacaaa atcaggtgtg
    19501 atcggcgcat cccctacacg accacgaatc ccatcggtac cgaaatattt acgattactc
    19561 atagcgtttg ttttcctttg cagacagagt ggcttccacc acccgcatcg cttctacggt
    19621 ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc attgcggcaa tgaccgcaca
    19681 ggccagacta ccgctcaggc gctcggacgg ccccacgttc agcagctgcc caatcatcga
    19741 ttttcgtgac atacccacca acagcggcag gttgaaatgg tgaaattcag ccaggcgcgc
    19801 cagtaatgaa tagttatggg agagattttt accgaaaccg aatccggggt cgagcaacaa
    19861 tttctctttt gcgatacccg cctgctcgca acgtgctatt tgctcaataa agtagcgatt
    19921 cacttctgca aagacatcgt catacttcgg agcttcctgc atggtttttg gatttccctg
    19981 catatgcatc agacaaaccg gtaaaccggt ttctgcagcc gcctccagag cgccaggttc
    20041 ggaaagggag cggatatcat taataatgtg agcgccaact ttcgctgact cacggatgac
    20101 ttctggtttg gatgtatcga ccgagatcca gacttcgaag cgttgagcaa ttgcctcaac
    20161 cacaggaata acacgttgca actcttcttc aacgctaact tccgccgccc ctgggcgcgt
    20221 ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg atcatcagat tcgcatgttt
    20281 caccgcatct atcagcgagt tatgcgtgcc accatccgaa aaggaatcag gcgtgacgtt
    20341 gaggatcccc attacgtgag gatggctaag gtccagtgaa gtaccctggg caaagagttt
    20401 catgatgtta tccctggtat gaattgataa gaaaaaaccc cggagcacgc cccggggttt
    20461 tcggtacaaa tacagtcatc tgatgcggga acttacttgt cgcctaactg ctctgacatg
    20521 gtgttacccg ggttcggcgt acgcggttca tcaaccggac gaggagcctt tggactacca
    20581 ttgtcgccag aattgttaga agcgcctggt tcttcccagc ccgctggcgg acgtacatcg
    20641 cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag tctcatattt catgagagca
    20701 tctttcatcg catgcagaat atccatattg tcggtcagaa gctgacgcgc acgattatag
    20761 ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac gtgcagtttc atcggacata
    20821 tgtttcgctt tcgctacgct acggccgagg aatacttcac cttcttcttc cgcgtacagc
    20881 agtggaccca atttctcaga gaagccccac tgggtcacca tgttacgtgc cagattggtc
    20941 gcaactttaa tatcgttgga cgcaccggta gaaacatgtt ccggcccata gatgatctct
    21001 tctgccagac gaccaccgta cagcgtagaa atctggcttt ccagtttctg acggctggcg
    21061 ctgattgcgt cgccttcagg caagaagaaa gtcacaccca gcgcacgacc gcgtgggata
    21121 atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc gaccgataat cgcatgaccc
    21181 gcttcgtggt aagccgttga ttctttctgc gcttccgtca tcaccatgga gcgacgttcc
    21241 gcacccatca tgattttgtc tttcgctttc tcgaactcaa ccatcgacac aacgcgtttg
    21301 ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt tcgccaggtc agcaccggag
    21361 aaaccaggag taccacgggc aatgattgcc gcgtcgatat cgggggccaa tggtacgcga
    21421 cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat ctggcaagcc gaccacaacc
    21481 tgacggtcga aacggccagg acgcagcagg gccgggtcga gaacgtccgg acggttagtc
    21541 gcggcgataa cgatgatacc ttcgttacct tcgaagccat ccatctcaac cagcatctgg
    21601 ttcagagtct gttcacgttc atcgtgacca ccgcccagac cagcgccacg ctggcggcct
    21661 acggcgtcga tttcatcgat aaagatgatg cacggtgccg ctttcttcgc ctgttcgaac
    21721 atgtcacgaa cacgggatgc acccacaccg acgaacattt ctacgaagtc agaaccggag
    21781 atagtaaaga acggaacttt cgcttcgcct gcaatcgctt tcgccagcaa cgttttaccg
    21841 gtacccggag gaccgaccat caggacgcct ttcgggatct taccgccgag tttctggaag
    21901 cggctcggct cgcgcagata ctctaccagt tcagcaactt cttcttttgc ttcgtcgcag
    21961 cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg tcagcatgcg cgctttgctc
    22021 ttaccaaacg acatggcacc tttgccaccg ccgccctgca tttgacgcat gaagaagatc
    22081 cagacaccaa tcagcaacag catcgggaac caggagatga agatagaagc cagcaggctt
    22141 ggttcttcag gcggttcacc gacaaccttg acgttcttgg tcaacaggtt atccagtaat
    22201 ttcggatcct gaaccggaat gtaagtggta taacggttac tatctttctt ggtaacgttg
    22261 atttcacgtc cgttgatacg cgcttcacga acctggtcgt tattgacctc ttgtaggaag
    22321 gtagagtaat ccaccttacg gccattagac tcgctgggcc caaagctctg gaatactgac
    22381 atcagcacaa cggcaatgac cagccagagt attaggtttt tcgccatgtc actcaaggga
    22441 ttaacctctt attacaactg tgttaaaaac agcgtcagga tactctatat ccagcatctt
    22501 tcaaactttc gtctgaaatc tcccggttag ggtttacgcc cggtcgctac aatatacact
    22561 tcccgcgaac gtgcacgaga agagtccggc ttacgaactt tgaccttcgt aaacagggag
    22621 cgaatttccc ttagatactc atcgaaacct tcgccctgga acaccttcac tacaaaactg
    22681 ccacctggcg ctaatacatc acgacacatt tctagcgcca gttccaccag atacatggca
    22741 cgggggatat ccaccgccgg tgttccgctc atgtttggtg ccatatcgga catgacaacc
    22801 tggactttgc tgtcgccaac gcgctccagc agtgctttca tcaccagttc atcacgaaaa
    22861 tcgccctgaa gaaagtccac accaacgata ggatccatag gtaaaagatc gcaagcgatg
    22921 atgcggcctt tgccgccaat ttgggtgacc acatattgtg accaaccacc cggagcagca
    22981 ccaaggtcga caaccgtcat tcccggttta aagagtttgt cactttgctg tatttcatca
    23041 agtttaaacc aggcacggga acgtaacccc tttttctgtg cctgttgaac atatttatcg
    23101 ctaaagtgtt cctgaagcca gcggctggag ctggcagaac gcttcttacc tgtcatttaa
    23161 ctttcccatg gggataactc atcgtaacca attgcgtaaa tttttacgcg cctatttggt
    23221 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
    23281 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
    23341 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
    23401 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
    23461 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
    23521 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
    23581 tcctaaagtt acacacattt gctgtgtaaa acgaggggtt ttccgcaggc aggagagcaa
    23641 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
    23701 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
    23761 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
    23821 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
    23881 gccaacgatg cgataagtct gttcttcgtc agaatccaga ttcagcaccg ttacggtagc
    23941 accgaaaata acgcgcccat tgttgggcat tttggtgaca tcaatcacct gcgcgttcga
    24001 cagcttggct tcgatgtctt taatacggcc ttcgcagaaa ccctgctgtt cacgagctgc
    24061 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
    24121 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
    24181 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
    24241 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
    24301 caaaaaaata ccgacccggg tacaagtccc aggtcagcta caattcacat tttgatagtc
    24361 attttaccct gaagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
    24421 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
    24481 attgaccagc tgtatagcgt tcagtgttca ggccgcgaat gttgatgagt acgttactca
    24541 actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
    24601 tattgattac cacagtcagc agatggcgct gcccgccagt acgcagaaag tgattactgc
    24661 gctggcggcg ttgattcaac tcggccccga ttttcgtttc accacgacgc ttgaaaccaa
    24721 aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc
    24781 gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa
    24841 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagcccc
    24901 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ctgccatagt
    24961 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
    25021 tatacgcgtg gcatcttatt accccgttac gatgttcagc caggtacgca ccctcccccg
    25081 aggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggcgacc tgaaccgctt
    25141 tacgctgacg ggatgcctgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
    25201 ggatggagcc agctatgccg gggcaattct gaaagatgag ttaaaacagg caggtatcac
    25261 ctggagcgga acactgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
    25321 taaacagtcg gccccgttgc acgatctgct taagattatg ctgaaaaagt cggacaacat
    25381 gatcgccgat acggttttcc gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg
    25441 gcgggccggg tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatattgg
    25501 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
    25561 catgatgcag gtgctgcaat acattgccca acacgacaat gaacttaact ttatctccat
    25621 gctgccgctt gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
    25681 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
    25741 attcattacc acagcgagcg ggcaacgaat ggcgtttgta caatatcttt ctggctatgc
    25801 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
    25861 gtataaagat atttatcaga acaattagtc aaaaagaaac cccggcacat ggccggggtt
    25921 ttgctgatta acgcttgtaa atgaactcaa cgccttcttc gtcgtcttcg tcccagtcat
    25981 catcccagtc ctcatcatcc tcttcagcaa tctcttcaag ctgctggcga tgataatcat
    26041 cccacatgaa ttcgactttc tctggctgtt tcgcttcttc agcctgcacg accgggtttt
    26101 caatgataaa ggtcatcaca tcccagcaga gatctttcac gcccagtcca ctcgccgcag
    26161 agatcagata atatttatct tcccagccca gcgcttcagc aatcgctttc gctttctctt
    26221 cagcttctgc cttatccagc agatcgatct tattgaacac taaccaacgc ggtttcgccg
    26281 ccagatcctg gctgtatttt tccagctcgc tgataataat acgcgcgttt tcaaccggat
    26341 cggtgccgtc aatcggatcg atatcgatga ggtgcaacag gacgcggcaa cgttccaggt
    26401 gcttcaggaa gcggataccc agacctgcgc cttccgcagc gccttcaatc agtcctggaa
    26461 tatcggcaac aacgaagctc ttttcgttgt ccattcgtac cacacccaga cttggcacca
    26521 gagtggtaaa cggataatcc gccactttcg gtttagccgc cgataccgcg cgaataaagg
    26581 tcgatttacc cgcgtttggc atccccaaca taccgacgtc agccagcagc atcagctcca
    26641 gcagcagctc acgcttatca cccggcgtac cgttggtttt ctgccgtggt gtacggttaa
    26701 cggacgattt gaaacgggta ttgcccagac cgtgccagcc gcccttagca accagcagac
    26761 gctgaccgtg tttagtcata tcgcccatgg tttcaccagt accttggtcg attatacgcg
    26821 tacctaccgg cactttaatc gtcacgtctt taccgcgctt accggtacaa tcgcggcttg
    26881 cgccgttctg accgcgctct gcacggaaag atttttcaaa acgataatcg ataagcgtgt
    26941 tcaggttttc gtcggcttcc atccatacgt caccaccatc accaccgtcg ccgccatccg
    27001 ggccgccttt cggaatatac ttttcgcggc ggaagctcac gcaaccatta ccgccatcac
    27061 ctgcaacgac cagaatcgat gcttcatcaa caaacttcat tttattctcc gtaaatcatt
    27121 cgcctgcgcg gggttgcgat accaccgttg tatgcttacg taatccgccc caaatacgat
    27181 gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa cgcaccgaga taacctaaaa
    27241 ggtttaacat cggtctggcg aagaaatcgg gccaggccag tgataaaaga tctgaaaaaa
    27301 acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac ctgcgctgcc tgccagcgag
    27361 ccatcgcttc cgccagggcg ccatatccta ccaatgtatt cagtccgcaa aaaattaaac
    27421 atgcgagctg ccagtggcta agctgcgcta tcactccagg ctttgccaga gggaagagcg
    27481 caattgtaca taaagcgtac agtaaaaaca ggatctgcgg tgaggccagc cgacgcaata
    27541 aaaccttttg cgccacgcca taactcaccc aaaccgtcgc cgcaccgacc ccaaagataa
    27601 ctccccaggt gtaatcggtg agctttgtaa atatctcgac cagactggtg ttaaaaaaca
    27661 tcaccaggcc gctcaggagc atcaatgccc ctacaacctg agtgctgcgc attttctctt
    27721 tcaggataaa tacgctggca accatcatgc caactggcga gagttgccca atcacctgcg
    27781 aagcggtcgg actcaggtat tgcaaggatg agctgaacag gatgaagttc ccaaacagcc
    27841 cggcggtcgc cactgccaac aaaatcaacc agcgtggctt acgaaacacg cgtaatggcg
    27901 gcaacctctt cttcaccgca agaatggcac caaggccaat actcgccatc aagaaacggt
    27961 aaaacacgat tgtcggaggt tccatcacct ccagcacctg cttcattgcg attggcaacg
    28021 ccccccagca aattgctgtg gtgagcgcca aaagaatgcc aatgcctgcc tgctgcttca
    28081 taccgtattc cctgcaaagc gcatttgcgg attaacgtcg caccatgcgc atttttaccg
    28141 gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct ttcatccgtt accgggacgc
    28201 gaaaaactta ttcagcttcg atgctgataa atttacggtt tttcgggcct ttaacttcga
    28261 atttcacttt accgtctgct ttagcaaaca gagtgtggtc acgaccgcaa cctacgttag
    28321 cgccagcgtg gaatttggta ccacgttgac gaacgatgat gctacccgcc agaacggatt
    28381 cgccaccgaa acgcttaacg cccaggcgtt tagcttctga atcgcgaccg ttacgtgtgg
    28441 agccgccagc ctttttatgt gccatttgaa atctctcctc aggtcttagg cgctgatgcc
    28501 agtaattttc acatcagtga accactgacg atggccctgc tgcttacgat agtgtttacg
    28561 acgacgaaac ttaacgattt taactttctc gccacgaccg tgagcaacaa cttcagcttt
    28621 gattacgccg ccatcaacga aaggaacgcc gattttgact tcttcaccgt ttgcgatcat
    28681 cagcacttca gcgaactcaa cagtttcgcc agttgcgatg tccagctttt ccaggcgaac
    28741 ggtctgacct tcgcttactc ggtgttgttt accaccactt tggaaaaccg cgtacatata
    28801 aaactccgct tccgcgcaca ccttttcaat gattcagagt gcgctataaa tattcacaat
    28861 agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt gctacagtca atacacgaag
    28921 aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca ttttttcagt acaatcactc
    28981 tatattccta accataaacc ctaagttgcc tttgttcaca gtaaggtaat cggggcgaaa
    29041 agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt taaccgcgca agatatggcg
    29101 ggtgttaatg cggcaatcct tgagcagctt aattccgacg tccagctgat caatcagtta
    29161 ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc cgatgattgc tgtactggct
    29221 gcacgagctg ttggctatga gggaaatgcg catgtcacca ttgctgccct gatcgagttt
    29281 atccacacgg cgactctgct gcacgacgac gttgtggatg aatcagatat gcgcaggggt
    29341 aaagctaccg ccaacgctgc atttggcaat gccgccagcg tgctggtagg cgattttatt
    29401 tatacccgcg cgttccagat gatgaccagc ctcggttcgc tcaaagtgct ggaagtcatg
    29461 tcagaagccg taaacgtcat cgcagaaggt gaagttctgc aactgatgaa cgttaacgat
    29521 ccggacatca ctgaagaaaa ctacatgcgc gttatctata gcaaaaccgc gcgtctgttt
    29581 gaggctgccg cgcagtgttc cgggattctg gctggctgta cgccggaaga ggagaaaggc
    29641 ctgcaggatt atgggcgcta tctcggcact gctttccagt tgatcgacga tttactcgat
    29701 tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg acgatctgaa cgaaggtaaa
    29761 ccgacgctgc cgctgctgca tgcgatgcat catggcacac cagaacaggc acagatgatc
    29821 cgtaccgcca tcgaacaggg taacggtcgc catcttctgg aaccggttct ggaagcaatg
    29881 aacgcttgtg gatctcttga atggacgcgt cagcgtgccg aggaagaagc agacaaagcc
    29941 atcgcagcgt tacaggtgct cccggacacc ccttggcgag aagcactcat cggcctcgcg
    30001 cacatcgctg ttcaacgcga tcgttaatcc cctcccctca tcccgcgcag cgcgcggggt
    30061 ggttccagta aattccataa aaacacttac tcagctctat aacacctgaa aaacaggctg
    30121 tgcatcttta tatattctga atattcacac tccttacagg aactttttag agcaatcggc
    30181 catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg actaacttaa
    30241 ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca tcattgcggg
    30301 tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt tgagttcctc
    30361 aacgctggcg aatgcattat cgcgcccatg gccgaaagga gagatgatta ttgcgaaagc
    30421 cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc agacccatga
    30481 gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat gagtggtctg
    30541 gcggtagccc cgcgaacggg gctgccagct ctcagacgat tactcgcctt tcacacgctc
    30601 aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac gatcgatgtg
    30661 ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca ccaggcttgc
    30721 tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa caccgtgaca
    30781 aataacggta ttgctttcga tttcggcatg cgcgcccata cggctaagct ccggcacatg
    30841 cataaagcgg ttttcaaaga ccgtttcggt gatgaatccg gtcccttctg ccaccaggtt
    30901 caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg cggtacgtac
    30961 gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt cgccgacttc
    31021 gatgtccgct ccagcgtcac gcagtttcgc cagcacggcg tcgagagtat ctggctgcgc
    31081 gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag taccggtttc
    31141 gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac cttcgatgac
    31201 gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga agttcgcggt
    31261 atcgacgatt tccggttcac gcgctgcgtt ttcaataatc gtggtacctt ccgccagggt
    31321 tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca cgatatgtgc
    31381 gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt tgatggtcgc
    31441 gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga tcgtacaacc
    31501 gccaggcagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca gtgcccagat
    31561 agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa cgtcgcgggc
    31621 atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta gcagcttcat
    31681 tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt cttccgccag
    31741 tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg tgacttcgcc
    31801 ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc tcagttaaca
    31861 attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt tgcgatcgcg
    31921 cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt tatccgcaat
    31981 atattccatc agcgggccat agaccgtctg ctgtttttta acccgactca tgccgtcaaa
    32041 caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt ggacttcctg
    32101 gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg gatcttcaat
    32161 catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat aaataagcaa
    32221 aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc ggacaggcgg
    32281 ttaacgccat atccggcctg aaaaaattta acgaggcaga acatcagcag gcaaattata
    32341 caatttcgcg agggtataca ctttgtcgtt taccccctga agcgtcacgt tgttgccctg
    32401 ctttttcgcc agatcgataa gatggagcag cagtgccagt ccacccgtat ccacgcggga
    32461 cacacggcta agatcgatgc aggtaatccc cttcaccgct tcctcacgca tttcccaaag
    32521 cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac ccgtctgcat
    32581 ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg ttgagaaatc
    32641 gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt tccccactcg
    32701 ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc ctgccagttt
    32761 cccgtctggg agtttttacg ccactggaag tccagacgca ccggcggacg gccattcggg
    32821 tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg ttctggcgca
    32881 atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg cttcaggtac
    32941 tcacggaaag cggcaaagta ggcttcacgt tgagcagggg tcgcactctt gtaatactgg
    33001 cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc ctgatcaaca
    33061 atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt caggcgatcg
    33121 aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc tgccgcggtt
    33181 gccgcactca gaggtgcaat caccagcaaa gcgaccatca ttaaacgttt aaacatgcgt
    33241 cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt attacctggc
    33301 gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc gtaaaggaac
    33361 tgaccaatga gatcttccag caccatcgca gacttagtgt cctgaattgt atcgccatcc
    33421 ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc cagatattgt
    33481 tcccccagca ggccggaagt acgaatgctc agcgaactgg tatctggaat gtggttataa
    33541 cgttgttcaa tttccagcgt tacgcgtggc agataggttt tcgggtccag cgtaatatcc
    33601 gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt caggccgcca
    33661 atgttatcga acgtcgcata aagcgtgtag gtcggttcag tacgtatgga cgtcacgttc
    33721 gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa gatacccacc
    33781 caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg cggtcagcac
    33841 aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg ttgcccggct
    33901 aatcccggca gacgtcggga tggcatcgta gccgttaaac aacgaaatcc acgtcaccgt
    33961 gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc agtcgacggc
    34021 attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga cgccgaccag
    34081 agatccgccc cagatcccca cggcgacgaa aataaccgtc aacagtggta atgaaataac
    34141 cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca tcatctccat
    34201 actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg ccgaaccagc
    34261 acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta gcgataacgc
    34321 caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat aaccttgcag
    34381 ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg acaggacgcc
    34441 gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg gtttgccgac
    34501 cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt taatcccttt
    34561 atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct ggtaaaagat
    34621 cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct atcccgtcca
    34681 gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg ccatgagcga
    34741 caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact tccggcacat
    34801 cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa atcagcttca
    34861 ccagtacgcc catggtgatg ggatcttgcc caacaaaagg ttcatcaaac atgatgagat
    34921 ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca ccagaaagtt
    34981 cagaaggcat tagcttagcc gctccacgca gccccacggc ctccagcttc atcatcactg
    35041 tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag gcgacgttgt
    35101 caaatacgtt catatcagtg aacaacgccc cggactgaaa taacatgctc atccgtttgc
    35161 gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg aaaaggatct
    35221 caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt ttaccgatgc
    35281 ccgatggccc catgattgcc gtgatcttcc ctcgcggcac ggtcagggaa atattatcga
    35341 agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa ttcgccacag
    35401 actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg ctcaatttaa
    35461 ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag ctggcatttg
    35521 ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt ctgaaaatct
    35581 tcagcggacc ggcgagtata cctgaagaaa ggatgttaga tgcttttagc tacggcactg
    35641 ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt tgccgcgtct
    35701 attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt ggtcagtatt
    35761 ggtacatcgt taccagaagt catcgtctcg cttgccgcgt ctctgcacga acaacgcgat
    35821 ttagccgttg gtacagccct cggctcaaac attatcaata tattgctgat cctcggtctg
    35881 gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga attaccctta
    35941 atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact tagtcgcagc
    36001 gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt taaacttgca
    36061 cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc agagctgccg
    36121 cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat catcatgcca
    36181 gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt tgccatcagc
    36241 gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga actggcaacc
    36301 gcaatagcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat cattggcgca
    36361 aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc aggagagatt
    36421 gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat tatttttgcg
    36481 ttgctgtgct ggcggcgctc cccacaaccg ggccgtggtg taggggtatt attaactggc
    36541 ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt tgaataactg
    36601 gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc aagcaggtaa
    36661 agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca tcaatcagaa
    36721 tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg tcatggggat
    36781 ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca ccggtacacc
    36841 ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg tcaccccaca
    36901 ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcacgg ccttaattcc
    36961 tgtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg agagcagcat
    37021 ggcgcgcgct gcagatgtgc atctgtgtgt taaagtagcg aaagaagcct gtccgttagg
    37081 gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc tcgctgtcgc
    37141 gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc caggcggcgc
    37201 actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg atgagatccc
    37261 gcatgttaag aaaacggcca gtctgcgtga cgcattgctg gaagttaccc gcaaaaatct
    37321 tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta ccgacggtga
    37381 tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg ccgatgtgat
    37441 gacgccgggg ggaatacgtg tgcgccctgg cattctggcc gttgaggcac tgaacttaat
    37501 gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac tcggtgtgtt
    37561 acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata aacaacaatg
    37621 agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga cgttatggca
    37681 aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc agatggcctg
    37741 atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga cggttatggc
    37801 attcgttgtg tgctcacctc tgatattgaa gtcgctatca ttaccgggcg aaaggctaaa
    37861 ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg gcagtcaaac
    37921 aaactgatcg cctttagcga tttgctggaa aaactggcga ttgccccgga aaatgtggct
    37981 tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt aagcgtcgcc
    38041 gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg cattgctggc
    38101 ggtcgtggcg cagtgcgcga agtttgcgac ttattactcc tggcgcaggg caaactggat
    38161 gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt gtgctatcac
    38221 tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc gcccaggtgg
    38281 tcgtcaacaa caatgatccc acctataaaa gcgagcatac ggacacgctc gtctataacc
    38341 cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat tccgatcagg
    38401 ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa atcccgacat
    38461 ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat ttatatggac
    38521 acgttgaagt caacgcactc gtgccggact ctcaacttcg cagaatcacg acggataacg
    38581 cgcagatcaa tctggtgacg caggatgtta cctctgaaga cctcgtcacg ttatacggaa
    38641 caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag aacgccgagc
    38701 tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag ccttaatctt
    38761 gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac cggagacact
    38821 gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg caacgtggtt
    38881 acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc cgacaaagtg
    38941 gttgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta cggtaaaccg
    39001 gcaacgttct accagatgca ggacaacggt aaaccggttg aaggtcacgc ttcccagatg
    39061 cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct gcagcaggtt
    39121 gatagcaaca ttaagggcga taagatcact tacctggtaa aagagcagaa aatgcaggct
    39181 ttcagcgaca aaggcaagcg cgtaaccacc gttctggtgc cgtcgcagct gcaggacaaa
    39241 aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat ggcaacatta
    39301 actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga cgtcagcctg
    39361 accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg taagaccacc
    39421 actttctata tggttgtagg cattgtgccg cgcgatgcgg gcaacatcat tattgatgat
    39481 gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg ctatctgcca
    39541 caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc ggtactgcaa
    39601 attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct gatggaagag
    39661 tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg tgaacgtcgc
    39721 cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct cgacgaaccg
    39781 tttgccggtg ttgacccgat ctcggttatc gacattaaac gcatcattga gcacctgcgc
    39841 gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact ggcggtttgt
    39901 gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc tacagaaatc
    39961 ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact ctgatagggt
    40021 agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa ggtttgcaac
    40081 tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt cgtctgttgc
    40141 agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt aatccgctgc
    40201 ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac agtgaaacgc
    40261 tggacaccgc cgatgcgctc gaacaaaaag agatgccgga agagctgccg ctcgatgcca
    40321 gttgggacac catttacacc gctggtacac catccggcac cagcggtgac tacattgacg
    40381 atgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac ctgatgtggc
    40441 aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct atcgtcgatg
    40501 ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa agtatgggcg
    40561 atgaagagat cgacatcgac gaggttgaag ccgtccttaa gcggatccaa cggtttgatc
    40621 cggtcggtgt agcggcaaaa gatctgcgtg actgcctgct aatccaactc tcccaattcg
    40681 ataaaaccac gccgtggctg gaagaggcca gattgatcat tagcgatcat ctcgatctgt
    40741 tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa gatgtgctga
    40801 aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg atccagactg
    40861 gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt cactggacag
    40921 tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac gcctcgatgt
    40981 gcaataacgc tcgcaatgat ggtgacagcc agtttatccg cagcaatctg caggatgcca
    41041 aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg agtcgctgta
    41101 tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa ccgatggtac
    41161 tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc gtgaccacgc
    41221 aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt tccagtcacg
    41281 tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg gtgaagaaat
    41341 taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc tctttgctgt
    41401 cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag tctttatcca
    41461 ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga agacactatg
    41521 cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga atttgttaca
    41581 gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta tgttgttctg
    41641 aaagtggaga aagtcaccca cacctcagat gcaacactgc atgtaaacgg tggtgaaatt
    41701 catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat tgataagctg
    41761 gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc cgggcaatta
    41821 gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt aatgctccga
    41881 gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa taatgataca
    41941 actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt ccactgtcag
    42001 agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact tagcctgccg
    42061 cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac cggtatcggc
    42121 aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgcgc cgttggcgtt
    42181 ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc ggtggactta
    42241 ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac tctgtcgctg
    42301 gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc ccagagcgat
    42361 gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc gtagttattc
    42421 ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat gatcgtcagc
    42481 ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat gggtttttac
    42541 tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaactct ggccgatcgc
    42601 gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc agaaatattc
    42661 gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactactgtt cctggatgcc
    42721 gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc gctttccagc
    42781 aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atttgctgga gcctctgcgt
    42841 tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct ggctgaaatg
    42901 ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga ccatggtctt tgagtctttc
    42961 ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg cttcttgccg
    43021 aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataaacc tgtcgccgcg
    43081 ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag ctatcttgaa
    43141 ctatggttac ctatgctgga aaccaacaac cgtagctact tgacggtcgc cattggttgt
    43201 accggcggaa agcaccgttc ggtgtatatt gcagagcaac tggcagacta cttccgctcg
    43261 cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc atgaccgtca
    43321 agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca atgaagctgt
    43381 ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa ggcaccgagg
    43441 ccgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga cggcagattg
    43501 aagttgaagc gaccggccca caggaagagg aagcactggc cgccgttatc gccctcttta
    43561 actctggttt tgatgaagat taatcttcat cactttgaca tacaactccc ttcaaactcc
    43621 ccccgacaat aagaaaatca cgtactgaaa tcgttctcaa tcaacgtcat ttgtacattt
    43681 tgtgcgcttt tcactttcag aagaacctta agaaaacctt aagagacatt gtttaggttt
    43741 tgtttaagtt aatcgaccat actggagatc gtcagaaaat atttccagga gatggcatga
    43801 ttcgcttatc tgaacaaagt cccctgggca ccggaagaca tcggaaatgt tatgcgcatc
    43861 cggaagatgc ccaacgctgt atcaagattg tctaccatcg tggcgatggt ggcgataaag
    43921 agatccgccg tgagttaaag tactacgcgc atcttggtcg ccggttaaaa gactggagtg
    43981 gaataccgcg ctatcacggt accgttgaga cggattgcgg aaccggttat gtctacgatg
    44041 tgattgcaga ttttgacggc aaaccctcca ttacgctgac cgaatttgct gaacaatgcc
    44101 gttacgaaga agacattgca caactgcgcc agctactgaa acagctgaag cgttatttgc
    44161 aggataaccg tatcgtgacg atgtcgctga agccgcagaa tatcctctgt caccgcatca
    44221 gtgagtcaga ggttatcccg gtcgtctgtg acaacattgg tgaaagcaca ttgattccac
    44281 tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga gcggttatgg aaacgattta
    44341 tcgcccaacc ggcgctggct attgccctgc agaaagatct gcaacctcgt gagagcaaaa
    44401 cgctggccct cacttcgcgc gaagcttaat ccagctggtg ttgttgcata aacggctcac
    44461 cgcctaactg atacatctgc cgtaaaatcc acgcctgacg gctacgcacg tagcccgatg
    44521 gcgaggagac tttgaaacga agtggattag gtaatacagc tgccagtaat gcagcttccg
    44581 accgggtaag tttgctcgcg ggtttgtgga aataacgttg tgccgcagct tcgacgccaa
    44641 acacaccgtc gccaaattcg gcgatattca ggtaaaccgt caggatacgc tttttgctcc
    44701 agactgtttc tatccccagc gttaatccag cttccagccc ttttcgtacc cagctacgcc
    44761 catcccataa aaagagattt ttggccgtct gctgagaaat cgttgaagca ccgcgaatac
    44821 ggttttcatt gcgctcgttg tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc
    44881 agtgctcagg aaatttctga tcttctgcgg caatcaccgc cagtcccatc cacggcgaga
    44941 tttgatccat actgacccag tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg
    45001 cgctgacctg tcgctcgacc attaccgctg agaagggaac aggcgcaaca ctaaacaacg
    45061 cgatgccccc gccccagaaa acggcgagga caaccattaa ccgcaaaaga aaacggcgaa
    45121 cgaaactaaa caccgttaag cggcttttac tcattcagcc agaaccagca cgcgggaaac
    45181 cagcttatca atgccgctcg ccgcttctgc aatgttctgc gccagcatat atgctggggt
    45241 ggtgacaatc ttattgtctt catcaaccac gatatcatcg acaggacagg gcacatgctc
    45301 cgcgcccatc tcttccagca cttctgcggt atcgatatca gtaccgatgg tcaaacgcag
    45361 cgggaaatcg aaaattttcg gcagcatcgc cggggcaata cacataaaac caagcggttt
    45421 tccggcctga tgcatcgctt gtgccagcgc ctttaattca cggtcaacgg tgcattcgct
    45481 accaagagtg gcaaaattgc ttaaattctt cgccgcgcca aacccccccg gcacaatcaa
    45541 cgcatccagt tcagcggcat cggcctgggc cagaggacgg atttcaccgc gcgttattcg
    45601 tgccgcttca atcagcacgt tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat
    45661 aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat
    45721 agctaacaac gtcaacaccg cttcatgaat ttcagaaccg tcatagacgc cgcatccgct
    45781 cagaattacg ccaattttct tcattgtgat catcctttcg caattgactg aaacacatta
    45841 atttttttaa taaaaatggt acgcatcaca catttaactg attcatgtaa caaatcattt
    45901 aagttttgct atcttaactg cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat
    45961 cataataatt tacggcgcag ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc
    46021 ggtctagccg gggtcatttt ttagtggctt ttgccaccca cgctttcagc acttctacgt
    46081 cgtgacgcca ctcttctttc atctcttcaa tccattcacc gacgttatct tcccaggcag
    46141 gaaggtccgg agactgaatt tgctgaccca gctgttgcag atggcgtaac cccactgacc
    46201 ccgccgcacc tttaatttta tgtccttcct ctacaatgcc ttttttatcc tgcgccgtca
    46261 gattcgactc cagcacgctg acatagcccg gcatcatctt ctcaaacacc gctaacccgt
    46321 cggtgatcag cttcggtcct acaagttcga gatactgttc cagcatggga atatcgagca
    46381 atgcttctga tttactgttc tcttctgtcg tcaccgtact ctcctcatca tcctgggtat
    46441 cccagaattt cttgatcatc gcggttagcg ccggaaccga aagcggctta ctcagcacat
    46501 catccattcc agcattgagg tactcttgtt tgtctttcag cacgttagcg gttaaggcca
    46561 ccagcggcgg taaatcctcg cgcggataac gcttcgtcag ttcacgagag atatccagcc
    46621 cggtcatatc tggcaactga atatccagca acaccaggtc gtattcgccc ggtttaaaca
    46681 tctccagcgc cgccttgccg gtcatggcga catcaacgct gttacctaat ttttccagca
    46741 cagaacgcgc aacaatcacg ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg
    46801 gtaaaggcat atcgtcttca tcaaacgcat catcgacctc ttctgctacc gacggtgcgt
    46861 ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct ggtaaccgta atatcgccgc
    46921 ccatattttt cgccagacga cgagaaacgg ccagaccaat accggtgccg gtggcaggtt
    46981 taccgccatg actgtctttc acctggtaat acatggcgaa aattttatcc agctcatcct
    47041 gcggaatgcc gataccagag tcttccactt caaaatgcag catatcgcct tcatcgtagc
    47101 gcacgcgcac ggtaacctgg ccttgctggg tgaatttgac ggcgttactg atgaggttcc
    47161 acaggatctg ccgtaaacgc gtcccgtcgg taatgacctg atgcggtaat ggcagcgtcg
    47221 gctccaggtt aaagcgcaat cctttttgtt gcgcctgcaa tgcggagaga ttttccagat
    47281 cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag ctggaccttg cgccgttcca
    47341 tcttatccat gtcgataata tcgttaaaga tattccccag cgtgacggcc gaaacatgga
    47401 tggtcttgag atatttttcc tgctcggcgg tgagttcggt atccagcaga atacggctca
    47461 ggccgacgat accgttcagc ggtgtacgca attcgtgact gatggtggag ataaacgtcg
    47521 ttttgtcgcg gctggcccgt tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc
    47581 gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc gtagtacggc actttacgga
    47641 tttcaaagca ggctttgcgc ccgtccgggt aatccagcca ctgttcatag gtcagtgaca
    47701 cattatgacg gaacactttt tcatcggttt caatgacttt tgcggcggct tccggtgagt
    47761 aaacatcagc aggtttcagg tgaaccagtt gtttttcgct ttttccggtc agcagctcca
    47821 tcgcgcggtt acagccggaa aactctttat cttcgttacg ataaaaaacc aggtcgggtg
    47881 aagcatcaag gaaggaacgt aagaatgagg attgctgctc gagctgaatt tgtgtctctt
    47941 cgcgctcttt gatttcaatt ttcagttggc cgaaggtttc ctgcagttct gcttccgctt
    48001 tttcacgaac ggcgatttcc tgatttagct gggcaatatt atcttttaac tgaacgttga
    48061 ggctcaaatc gcgctcgcgc atctcctcca gtttttgcac cagccgtgac agacgttgtc
    48121 gtgactcctc cagttgctcg acgaccaccg atagaaagta gaccgcccac ggcgtaatca
    48181 gcaaaccaaa gaagatagaa cgaataacat caatgctttc gacctgacca tgcagcacca
    48241 tggttaccgc catttgtacc acaatggcaa gaacgacgag ggccagcgcc agcaacattg
    48301 agaagcgcac cagacctaac ttcatcatca ggtcaacata atactgcgcc agcagacgaa
    48361 tttgcttcat tagggaattc cttcacgaca acctgcccca ataataccca attctgacag
    48421 ttacgttata aattgtgtga gaaatgcgga attcccctca acgacctact ccgttggagg
    48481 tatccacgga cgttccagcg ccgatccctg tacgccatgt tcattcagat agcgatccag
    48541 ctcgaccatt cccgtccatc gattttcgca ccacagagga gcaagcagcg tcgggcgacg
    48601 ggcgctggcg gaaatgcggt ggtagatcac ttccggtggc gtatggcgaa tcatttctcc
    48661 ggcagtgagc gtgtaatcct ccagttcaat accgttcaaa cgccccgctt cccaggcttt
    48721 cgccataatg ctgcctttca caatatgcag cggatgcagc tttatgccgt ccacgccggt
    48781 ttcaaccact cgctccagcg tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac
    48841 aattaagtgg gaacacacct tcagcccgcg ctgacgtgcc agctgggttg tacgctgata
    48901 acaggcaaaa tcatggccgc ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag
    48961 ccccagctcc agccacactt cgtaaccctg gtccttatat tcgcaaagca gatccagcac
    49021 cgcatccggc acgcagtccg ggcgggtacc aacacacaaa ccgacaatat tggcctggct
    49081 caccgcctgc tgatacatag aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc
    49141 ctgaaagtag gccagatagc gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg
    49201 ctcggtaatg gaacgatgct gctgcgcttc atcggcaaac gaggcaacat tacagaatgt
    49261 gcagccacca cgcccgatgg taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt
    49321 cagcttatgc accttttgcc cataacgacg ggtgagatca ccaccaaaca tattgactaa
    49381 tttctgtaac tgcataatct gatagaccgc gccttgaaaa gaggccaaag cctgccattt
    49441 ttagctcaat tcggcgatga cctggatcaa tcgtcccggc ctgcttttat caactgcata
    49501 atcaatcaaa attaccgaaa tttcatgcat aatcacataa atcacttttg cttatcttgt
    49561 gtcagatttt tttatctcct tatggatttt aggcaaaaac agtagcatga aacgtcatta
    49621 ccaattaagg cagtataaaa tgctggcttt ctcgtcagtt caaggcagga taagggttaa
    49681 cacgccttta tgacagtcag gaattgactg tttctctaac gacttccctt ttagccttaa
    49741 agataaaatc tattttaatt tcagtcattt aataaagaat tttgcgctaa agcacatttc
    49801 tgtaccaata agcttgccat ttgacctgta tcagctttcc cgataagttg gaaatccgct
    49861 ggaagctttc tggatgagca gcctgctcat catatttatg cagtaattga gatcccctct
    49921 tcaccgtatt aaccgatgcg aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga
    49981 cacgcaaacc ccgtcgccac gctctttctg tgcccgtgcg cagcggttcg gaagtggggt
    50041 tcccgcagag cctgggggag gttcacgata tgttgtacga taaatccctt gagagggata
    50101 actgtggttt cggcctgatc gcccacatag aaggcgaacc tagccacaag gtagtgcgta
    50161 ctgcaataca cgcactggcc cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa
    50221 ccggcgacgg ttgcggcttg ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc
    50281 aggagcgcgg ctggcgttta gcaaaaaact acgccgtcgg gatgctattc ctgaataaag
    50341 atcctgaact cgccgctgcc gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct
    50401 tgtcgattgt gggctggcgt gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc
    50461 tctcctctct gccacgcatt gagcaaattt ttgtgaacgc cccggcaggc tggcgtccgc
    50521 gcgatatgga gcgccgtctg tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg
    50581 acaaagactt ctacgtctgt agcctgtcga atctggtgaa catctataaa ggtctgtgta
    50641 tgccggcgga tctgccgcgc ttttatctgg atcttgcgga cctgcgtctg gaatcggcca
    50701 tctgcctgtt ccaccagcgc ttctccacta acaccgtacc gcgctggccg ctggcgcagc
    50761 cgttccgcta cctggcgcat aacggtgaaa tcaacaccat caccggtaac cgccaatggg
    50821 cgcgtgcgcg tacctataaa ttccagacgc cgcttatccc tgatctgcac gacgccgcac
    50881 cgttcgtcaa cgaaaccggc tctgactcca gttcgatgga taacatgctg gaactgctgc
    50941 tggcaggcgg gatggatatc atccgcgcca tgcgtctatt agtaccaccc gcctggcaga
    51001 acaacccgga tatggacccg gaactgcgtg ccttctttga ctttaactcc atgcatatgg
    51061 agccgtggga tggcccggcg ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc
    51121 tcgaccgtaa cggtctgcgt ccggcacgct acgtcattac caaagacaag ctcatcacct
    51181 gcgcctctga agtcggtatc tgggattatc agcctgacga agtggtcgaa aaaggccgcg
    51241 tcgggccagg cgaactgatg gtgatcgaca cccgcagtgg gcgtattctg cactcggcag
    51301 aaaccgatga cgatctgaaa agccgccatc catataaaga gtggatggag aaaaacgtcc
    51361 gccgactggt accgtttgaa gatctgcccg atgaagaagt gggtagccgc gaactggacg
    51421 acgacacgct tgccagctac cagaaacagt ttaactacag cgcggaagag ctggactccg
    51481 taattcgcgt actgggcgaa aacggtcagg aagcggtcgg ttcgatgggc gatgataccc
    51541 cattcgccgt gctctccagt cagccgcgca ttatttacga ctacttccgc cagcagtttg
    51601 cccaggtgac taacccgcca atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca
    51661 ccagtatcgg tcgtgaaatg aacgtctttt gcgaagcaga gggccaggcg caccgtttaa
    51721 gctttaaatc gccgattctg ctctactccg atttcaaaca gctcacgacg atgaaagagg
    51781 agcactaccg cgcagatacg ctggatatca cctttgacgt cactaaaacc acgctcgaag
    51841 cgacagtcaa agagctgtgc gacaaagccg aaaaaatggt acgtagcggc accgtgctgc
    51901 tggtgctctc cgaccggaat atcgctaaag atcgcctgcc ggttccagcc ccgatggcgg
    51961 ttggcgcgat ccagacccgt ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg
    52021 tcgaaaccgc cagcgcccgc gatccgcacc acttcgccgt gttgctgggc ttcggcgcga
    52081 cggctattta tccatacctt gcctatgaaa cgctgggccg cctggtagac acccatgcga
    52141 ttgccaaaga ttatcgtacc gtgatgctca actaccgtaa cggcatcaac aaaggcttgt
    52201 acaaaatcat gtccaaaatg ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt
    52261 ttgaagcggt cggtctgcac gatgatgtag tgggcctgtg cttccagggg gcggtcagcc
    52321 gcattggtgg ggcgagcttt gaagacttcc agcaggatct gctgaacctg tcgaaacgtg
    52381 cctggctggc gcgtaagccc atcagccagg gcggcctgct gaaatacgtc cacggcggcg
    52441 aataccacgc ttacaacccg gacgtggtgc gcacgctgca acaggcggta caaagcggcg
    52501 agtacagcga ctatcaggaa tacgcgaagc tggttaatga gcgtccggca accacgctgc
    52561 gcgatctgct ggcaattacg ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg
    52621 caagcgaact gtttaaacgc tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg
    52681 aagcccacga ggcgctggcg gaagcgatga atagcatcgg cggtaactcg aactccggtg
    52741 aaggcggcga agacccggcg cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg
    52801 cttctggtcg cttcggcgtt actccggcgt atctggtcaa tgccgacgtc attcagatta
    52861 aagtcgccca gggcgcgaag ccgggcgaag gcggtcagtt gccgggcgac aaagtcactc
    52921 cttacatcgc caaactgcgc tattcggtgc ccggagtgac gctgatctcc ccgccgccgc
    52981 accacgatat ctactctatc gaggacttag cgcagctcat tttcgacctc aagcaggtta
    53041 acccgaaagc gatgatctcc gtgaagctgg tttccgaacc gggcgtaggc accatcgcga
    53101 ctggcgtggc aaaagcttat gccgatttaa tcaccatcgc aggctatgac ggtggcaccg
    53161 gcgcaagccc gctgtcgtca gtgaaatacg caggttgccc gtgggagctg gggcttgttg
    53221 aaacccagca ggcgctggtt gccaatggtc tgcgtcataa gatccgtttg caggtcgatg
    53281 gcggcctgaa aaccggtgtt gatatcatca aagcggcgat tctcggcgca gaaagcttcg
    53341 gcttcggcac tggcccgatg gtggcgctcg gctgtaaata tctacgtatt tgccatctga
    53401 acaactgcgc aacgggtgta gcaactcagg atgacaaact gcgtaagaac cactatcacg
    53461 gcctgccgtt caaggttacc aattactttg aatttattgc ccgtgaaacc cgcgagctga
    53521 tggcacagct gggcgtaacg cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag
    53581 aactggacgg tttcaccgcc aaacagcaaa aactggcgct gtcgaagctg ctggagaccg
    53641 ccgaaccgca tccaggtaag gcactctact gcaccgaaaa caacccaccg tttgataacg
    53701 gcctgctgaa cgcgcagttg ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca
    53761 aaaccttctg gttcgatatt cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct
    53821 atatcgccca gacgcacggc gatcaggggc tggcagccga tcctatcaaa gcgtacttca
    53881 acggcaccgc aggccagagt ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga
    53941 ccggggatgc caacgactat gtcggtaaag gcatggcggg cggcttaatc gccattcgtc
    54001 ctccggttgg ttccgccttc cgcagccatg aagcaagtat tatcggcaac acctgcctgt
    54061 atggcgcgac cggtggtcgt ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc
    54121 ggaactccgg tgctatcacc gtggtagaag gcattggcga caacggctgt gaatatatga
    54181 cgggcggtat tgtctgtatt ctgggtaaaa ccggcgttaa cttcggtgcg ggcatgaccg
    54241 gcggcttcgc ttacgttctc gatgaaagcg gtgatttccg caaacgcgtt aacccggaac
    54301 tggtcgaggt cttaagcgtt gacgatctgg cgatccatga agagcatctg cgcggtctta
    54361 tcaccgagca tgttcagcat actggctctc agcgcggtga agagattctg gcgaactggt
    54421 caaccttcgc cactaaattt gcgctggtta aaccgaagtc cagtgatgta aaagcactgc
    54481 tgggtcatcg tagtcgtagc gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa
    54541 caatgagtca gaatgtttat caatttatcg acctgcagcg cgttgatccg ccaaagaaac
    54601 cgctgaagat ccgcaaaatt gagtttgttg aaatttacga gccgttttcc gaaggccagg
    54661 ccaaagcgca ggctgaccgc tgcctgtcgt gcggcaaccc atactgcgag tggaaatgtc
    54721 cggtacacaa ctacatcccg aactggctga agctcgccaa cgaggggcgt atttttgaag
    54781 cggcggaact gtcgcaccag accaaccccc tgccggaagt ttgcggacga gtctgcccgc
    54841 aagaccgtct gtgcgaaggt tcctgcactc tgaacgatga gtttggcgcg gtgaccatcg
    54901 gcaacattga gcgttatatc aatgataaag cgttcgaaat gggctggcgt ccggatatgt
    54961 ccggcgtgaa gcaaaccggt aaaaaagtgg cgattatcgg cgcaggcccg gcaggtctgg
    55021 cgtgtgcgga tgtcctgacg cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag
    55081 aaattggcgg gctgctgacc ttcggtattc cggccttcaa gctggaaaaa gaggtaatga
    55141 cgcgtcgccg tgaaatcttc accggcatgg gtattgaatt caaactcaat accgaagtgg
    55201 gccgcgacgt gcagctggac gatctgctga gtgattacga tgccgtcttc cttggcgtcg
    55261 ggacttatca gtcaatgcgc ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag
    55321 cgctgccgtt ccttatcgcc aacaccaaac agttaatggg ctttggtgaa acccgcgacg
    55381 aaccgttcgt cagcatggaa ggcaaacgcg tggtggtcct tggcggtggc gacactgcga
    55441 tggactgcgt gcgtacgtcc gtgcgccagg gagcgaagca cgttacctgt gcctatcgtc
    55501 gtgatgaaga gaacatgccg ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg
    55561 tagagttcaa attcaacgtc cagccactgg ggattgaagt gaacggtaac ggtaaagtca
    55621 gcggcgtaaa aatggtacgt accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg
    55681 cggagatcgt tgcaggttcc gaacatatcg ttccggcaga tgcagtgatc atggcgtttg
    55741 gtttccgtcc acacaacatg gaatggctgg caaaacacag cgtcgagctg gattcacaag
    55801 gccgcatcat cgccccggaa ggcagcgaca acgccttcca gaccagcaac ccgaaaatct
    55861 ttgctggcgg cgatatcgtc cgtggttccg atctggtggt aaccgctatt gccgaaggtc
    55921 gtaaggcggc agacggtatt atgaactggc tggaagttta agctctgtcc cagcgccgcc
    55981 tcctcagaaa agagtcggcg ctgaaattct cctttctttc tggaaagcgc ctcgcataat
    56041 ctgaagaaaa ccaatgtaat acgctcatct ggattgttac cggtagatgc ccggcgtata
    56101 gctgattcca ttattcaacg cagcgccata taaagggaca gataatggaa tcgctctcag
    56161 agagaacctc aacaggctac cagcaaatcc acgacggtat tattcatctg gtcgatagcg
    56221 cccggacgga aacggtacgt agcgttaacg cgctgatgac cgcgacatac tgggaaattg
    56281 gccgacgaat tgtcgaattt gaacaaggtg gcgaggccag ggctgcgtat ggtgcgcagc
    56341 taatcaagcg actatcaaag gatttaagtc taaggtataa gcgtgggttc tctgcaaaaa
    56401 acttacgcca aatgaggctt ttttacctct tttttcaaca tgttgaaatt cgccagacag
    56461 tgtctggcga attaacacct gccctggtcc acctacgtcc gtttactttc cgttaaaaac
    56521 gctgacgccc gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag
    56581 ctagaacggc aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca
    56641 gccatgctgc aacaacacgc tcctaccgag acgcatattc ttccgcaaca ggcgatacgc
    56701 gatccctttg tgctcgaatt tctggaattg aaagatgaat attcagaatc tgatttcgag
    56761 gaggcgctga tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt
    56821 gttggtcggc agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt
    56881 ttccaccgcc gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat
    56941 agcgatgccg gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg
    57001 gatgaaaatc cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat
    57061 tatgctctgg caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct
    57121 gatgagaaac gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat
    57181 agacgccgtt aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa
    57241 cgaactaagt tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct
    57301 ccaggctcgc cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac
    57361 attcccggtt ttaagatgat ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca
    57421 tcctcatggt ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt
    57481 tcctcaccgt ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc
    57541 tcgacgggcg attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat
    57601 tcgtaacgac ccggcgcttt ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc
    57661 tgtaacgcag gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt
    57721 tttctccctg ggccaacagc gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg
    57781 ccagtaaatc aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca
    57841 gatacccttc tgccagccca acgctgccac cgaccaccac gcactggcaa tcagttgtgg
    57901 ctttaatatc agcgatcagc cttgcaagcg tacgtgcgga gcggtgaatc agctgctgcg
    57961 cctgctcgtc gccctgcccg gcgcgtgtga aaatagttct cgcatccgcg cccgccagtt
    58021 caccctgcgc tgccgccgca atgccgcgac cagaagcaat cgcctccacg caacccgtgc
    58081 gtccacagcc acagaccggg ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca
    58141 gaccgccagg gccggtaagc agtttgccgc cgctcactac accgccgccg acgccagtgg
    58201 aaacggtgat aaagaccata tcggttatat cgccttccag cgcctgatac tctgcccatg
    58261 ctgcggcctg cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt
    58321 tgactaacgg aaagtgtagc aatccaccga gattatgcgg attcagcgct agcaaactgc
    58381 cgtcgcgaat aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag
    58441 agactaatgc ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa
    58501 gttcacgacg atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac
    58561 cgccgatatc aatcgccagc gtggtcatag caccgccttt ttcatcgctg tgttgtacca
    58621 ctgacaaatg tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg
    58681 gcgcatcgca tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcaccctaca
    58741 tccggcgtcg ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt
    58801 ggtatagcca gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc
    58861 gtcttccggc gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag
    58921 cagcgtttca acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc
    58981 gcccgcctgc gccagcgcat caacatcttc aatataggcc gtgatgcgta ccggagaatc
    59041 ctccagatcg cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg
    59101 cagatttgcc acaccttcaa tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc
    59161 catggcggcg acgatttcgg gtttatcgag cgggctgtcc ggaaccggct ggcaggagac
    59221 aatcaggcca ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct
    59281 tcccttagcg aaaggcccgg tacatagacc gggcaacagg attaactttt ggttttgact
    59341 aaatcgtttt tggcgctgcc aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg
    59401 tcatgagtac gcaatgcttc cgggcgcagc caacgctgaa cgcgggaagg catatccaga
    59461 ccaatcagca ggatcaccac aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc
    59521 agatccagac gttgagcgat caacgcgccg atgattgggg ccagtgcgcc gcccaatgca
    59581 ccaacgttgt aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca
    59641 atcagtttcg gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac
    59701 agaccgagca cccagacgtt tgcgccgcca atcgcaaata ccggaataat caacagctgc
    59761 gaggccaaca ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagccg
    59821 ccgacacagc accccaccgc agccccaaag ccgctaaaga acagcacatt ggcgacagta
    59881 tgcgggttat aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat
    59941 gagtagagga aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgttta
    60001 cccgtacttt gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg
    60061 acgatcgcgg cattttgcag gttaccggcg aagcaaaacc acagcgcagt agccgccgcc
    60121 agtgtcatta cgatattggc aatgcgatgt tcaccacggt agagaatatc taccatcgtg
    60181 cgtactggtg ctttacctgc atgtttctct ttccagtctt ccgcttccgg gatgttttta
    60241 cgcagccaga gagcaaagat gataggcaaa atgccgataa agaacagcgc acgccagccc
    60301 cagaccggaa ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct
    60361 gaaatcagaa aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag
    60421 gtggcgctgg aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac
    60481 atggtgatgt agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg
    60541 ctggtgacca ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg
    60601 ccgaaccagc gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc
    60661 ccgaattcac cttgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca
    60721 agcagatatc ccaaccaggc agcggaaaat gcccgccatt gtgcacgatt gagatggcga
    60781 taccacggga tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc
    60841 acacgctttg gtatgaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg
    60901 ggcgagcgag gggaaaacaa ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt
    60961 cagttctggc agatattttt catctaccgg tccaaacggt ttgcggcaca gcggcacaga
    61021 aacgacatcc atataatgga ggacagtttt caggccgcgg aatacgcccg ttttgatcag
    61081 taaatcaatg actttattgc attcagtttg cagtttctgc gcggtctgga tatcgccttc
    61141 tttcagcgcc ttaacgatcc cctgatagcg ccagcccatg atgttgtagg tactaccgat
    61201 accaccatca gcgcccgcca gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag
    61261 cacaagatca ggatgttcac gacggatctg ctccatctga tagagatcgc cagaagtctg
    61321 tttcagcgca ccgacgccag gcaacgtaac cagggtgttg atctgatcca gggtcagttt
    61381 taccccactc agggctggaa tgttgtacac caccatcggc aaaccatccg ctgaatcaat
    61441 aattgcccga tagtgatcgc agtgttcttc aaagctgaaa ggatagtaga acggcgtgac
    61501 ggcggagacg gcatcgaagc cataacgttt agccgatgcc gcaagttgtt ggctttcggc
    61561 ggtgctgacg caaccgacgt gggcgatgag tttaatctta cctttcgcct cttcggcgac
    61621 gatttccagt acctgttcac gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc
    61681 acccacgtat aaaccgtcga tgccctgctg aatattgaac tgaaccaggc gacgcagact
    61741 cgctttatcc agtgcttgtt gttgatcaaa aggagtcagg agtgcagcca ttacgccacg
    61801 taaattcgtt gccataaata cctctgaagt gatgcttgtc tgataaacga tataccttta
    61861 tacctgttat accagatcaa ttaagcaaca ccccatacag aaagcttata atgcgatctg
    61921 cttcactaaa gtggcattat ttctttttgt tggtggtctg accgaaagcg tgccaggtag
    61981 cagagacgct gttgagatgc gattgcaacg cacgatcggc ttcgtcagga tcatgacggc
    62041 ggatcgcatc aacgatcgca atatgctgtt gataactaac gttgttatgt tcgtgcagtg
    62101 cctgatcggt aaccgttggg cgtgcggcaa taagccagtc gagcagggca acgtggatcg
    62161 ccatgaagat tgggttaccg gggatctccg ccagcacgcg gtggaaatca acgtctgaac
    62221 gaatgaatgc cgcgttgtta tccagcgact gactgttaat ttccagtgct tttgccagca
    62281 aatcgatttg ctcatcggtg gcatgttcag ccgcatagcg caccaggctg gattcaaaga
    62341 acagacgtaa ttgttcgaaa tgggcaatcc caccaggatg agaaaggaaa tctttcgcca
    62401 tgccggaaag ctcgccgatg atggtgtccg cagaaggacg tgagacgcgg gcacgttcgc
    62461 cgttgttgat ttgcaccaga cctttgcgtt ttaacgctgc cagcgcttcg cgcacagaag
    62521 gacgcccgac gttaaagaac gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc
    62581 caaattcacg acggcggatc atctgttcca gctcttcttc caccatttcg gagagttttt
    62641 tacgcgccag cgggcggcta cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt
    62701 gcgaatcaaa tgcgttcata aggcccattc tgtaaggtca gtgtgattaa catcatcagt
    62761 gacatcctat cacaggattg aaagtagggg aaaatggcag gtttttctct ttgtgcctca
    62821 tcattaccat aattaacgga ataattaact attgcgaaaa attaatgtaa cgcagataaa
    62881 aacatcccgt ttgaattatt tataagacta ttcacgagca ttatgaatat tatgaatgtg
    62941 ttcttacaaa ataatcataa gcgcatattt tttaatgaaa aatcacctca cctacaatta
    63001 aaaacacgac atccgcacca taaatagact tgcaaaaaat ataacatcat tgttttcaat
    63061 ctgccgttta tgggattgac cgttttcttt tgacacggag ttcaacaatg ttcggcataa
    63121 ttatatctgt catcgtatta attacgatgg gctatttgat cctgaaaaac tacaaacctc
    63181 aggtggtgct ggctgccgca ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg
    63241 gtggtgtact cgatcccgcc aaaagcagcg gctacttgat cgtcgatatt tataatgaaa
    63301 tcctgcgcat gctgtccaac cgcattgccg gattggggct gtcgattatg gcagtgggcg
    63361 gttatgcccg ctacatggag cgcacaggag ccagtcgcgc gatggtgagc ttgctaagcc
    63421 gcccgttaaa actcattcgc tcgccgtata ttattctgtc ggcaacttac gtcatcggcc
    63481 aaatcatggc gcagtttatt accagcgcct ccggcctggg tatgttgctg atggtcacct
    63541 tatttccgac gctggtgagt ctgggagtaa gtcgcctctc tgcggtggcg gttatcgcaa
    63601 ccacgatgtc cattgagtgg gggattctgg aaacgaactc catttttgca gcccaggtcg
    63661 cgggaatgaa aattgccact tacttcttcc actaccagct tccggtcgcc tcttgcgtca
    63721 ttatctcggt ggcgatctcc cactttttcg tgcaacgcgc ttttgacaaa aaagataaaa
    63781 atatcaatca cgaacaggca gagctaaaag ctctcgataa tgtcccgccg ctctattacg
    63841 ccattttacc tgtgatgccg ttaatcttga tgctcggctc gctgttcctc gcccacatcg
    63901 ggctgatgca gtcagaactg catctggtgg cggtgatgtt actgagtttg acggtgacga
    63961 tgtttgttga gttcttccgc aagcataact tgcgcgaaac aatggacgat gtgcaggcgt
    64021 tttttgacgg catgggtacg cagtttgcca acgtggtaac gctggtggtc gcgggtgaaa
    64081 tatttgcgaa aggcttaacg acgattggca ctgttgatgc ggttatcagg ggtgcggagc
    64141 attctggtct gggcggtatt ggcgtgatga ttattatggc gctagtcatt gccatttgtg
    64201 ccattgtgat gggctctggc aatgcgccgt ttatgtcatt tgccagtctt attccgaata
    64261 tcgcagccgg actacatgta ccagcggttg taatgattat gccgatgcat tttgccacga
    64321 cgctagcgcg cgcggtttcg ccgattactg cggtggtggt cgttacgtca ggaattgcag
    64381 gcgtttcgcc ttttgcggtg gtgaagcgaa cagcgatccc catggcagtc ggtttcgtgg
    64441 tgaatatgat tgccacaatc acgctatttt attaagccat taaaagacaa aacaggccac
    64501 cagggcctgt ttttgtatta cttcacaacg cgtaatgccg gtcgaccacc gcgtggtggc
    64561 tgcggaggtt catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata
    64621 accgacataa cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct
    64681 tcatcgtagg cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata
    64741 gccagcacgg cagccagcgg cacagaaacc tgacgcggaa tgccgccaaa gcgcgcgtta
    64801 aagcgtacct catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt
    64861 acgatttgcc cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc
    64921 accaccaagt gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga
    64981 tagggacgac gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca
    65041 tttcacgttc tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca
    65101 tatagccttt cagctctttc gcacccgggc cgctgaactc gatgcccagt tgcggcagac
    65161 gccacagcag cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg
    65221 gcttctgacc gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg
    65281 catctgcttc agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt
    65341 cgatgcgatg catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg
    65401 gcggatgcgg gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca
    65461 gctcacgatc caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag
    65521 gcggattgtc cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa
    65581 tgcggacctg atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt
    65641 tgttggcagc gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc
    65701 caccaggtgg ccagtcagaa gttgtgttac ccagtaagga acgactctct ttgttcgaaa
    65761 atcaaacaaa aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca
    65821 gattttgcga cctttgtggt gagtcgatgc cggaaatggg gaaaaagaga tgcgctttag
    65881 tctgaaatag ttgacttagt cccttattgg cgatgtggtt tttgttttgc ctgtctgtca
    65941 gacggtagca aaaagcaact ttccaatttt tacgctgatt cagaatttaa ccataaaaaa
    66001 acccgccgaa gcgggttttt tcgcaaattg ttttctgccg gagcagaagc caattaacgt
    66061 ttggagaact gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga
    66121 cgagcgtcac gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat
    66181 tccatcagag cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca
    66241 cctttaacgg tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga
    66301 cgaactacca tgcgagcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt
    66361 acgattttac cgttgcccgg tttgatgaaa acgcgagctg cggaactttt gcggcgacca
    66421 gtgccgtagt attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc
    66481 ggttgctgtg ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata
    66541 gcacgaccca gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca
    66601 ggacggcgag caatcatctc ttcaaaggtc gcttgtttga taccaccgat gtggccggtg
    66661 tggtgatagt acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc
    66721 agaacgatga tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg
    66781 cgcaggcgac gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca
    66841 acataccagt cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta
    66901 cccaataaat agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac
    66961 gacaaagtcc ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg
    67021 gaaaaaaact ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc
    67081 gacccctttt tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact
    67141 cttcgctttg catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat
    67201 cgccctgata aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct
    67261 cgtaaaactc atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca
    67321 taactggtac atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat
    67381 gctgactgcg ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga
    67441 cgcccattgt cgccaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcg
    67501 cccccgccag cgccaaccat agtttatcca tctgcgcccg ggtttcatcg ttgagtggcg
    67561 aaagccacag atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt
    67621 tcattacatc acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca
    67681 ggccatttcg ataaagttca tccggcggaa tatttgacgt cgctaccagg gtaatgccgc
    67741 gggcgaacag ggctttcatc agaccgccaa gtagcatggc gtcggtaata tcagaaacaa
    67801 aaaattcgtc aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca
    67861 gcggatcggt ctgcccctgt aaggcagtta gctcttcgtg cacccgcagc ataaaacggt
    67921 gaaagtgcag gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc
    67981 aggttttccc gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg
    68041 tgtcttcgcg tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg
    68101 ctggtggcct gctattgatg agttcttgat aaataatttc caggcggctg acggcctctt
    68161 tttgaacgtc gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg
    68221 gggtaacgct ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac
    68281 ggttgacgaa aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt
    68341 ggaattaacg gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct
    68401 acggaaacaa aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag
    68461 tcgtcggcat cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc
    68521 aacaggcgtt gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag
    68581 agctggttag ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc
    68641 gccagctgta tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg
    68701 aagcaaaccc gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg
    68761 tgcagatgcc tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc
    68821 gcgactaatt tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact
    68881 atcatttaat ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt
    68941 tgaatctctt ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaacccagc
    69001 tgttgagtgc attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg
    69061 cgtcgattcc aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg
    69121 aaaaagtgct tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga
    69181 aaatcccgga agaattcaaa aagttttttg gtgatgattt accggatcaa cctgcacaac
    69241 ccttcgaagg tttaggttcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca
    69301 acaaccatgt gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt
    69361 ttgatgcaaa actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa
    69421 acccgagcaa attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt
    69481 ttgccgtagc ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt
    69541 ccgcattagg ccgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg
    69601 cttccattaa ccgcggtaac tccggcggtg cactattaaa ccttaacggt gagttaattg
    69661 gcatcaacac tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc
    69721 ccagtaatat ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg
    69781 gtttgttagg catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg
    69841 acgtgcagcg tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcaaaagcgg
    69901 gcgtcaaagc gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg
    69961 agttgcgctc tcgtatcgcg actacagagc cgggcacgaa agtgaagctt ggcctgctgc
    70021 gtaacggcaa accactggaa gtagaagtga cgcttgatac cagcacctct tcgtcggcca
    70081 gcgctgaaat gatcacgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag
    70141 atggcggcaa aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg
    70201 gcttgcaaaa agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg
    70261 aaatgcgtaa agtgctggcg gcaaaaccgg ccatcatcgc cctgcaaatt gtacgcggta
    70321 atgaaagcat ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg
    70381 atgtccggtt aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat
    70441 gtttgtgaag ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt
    70501 tgccatgcct tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga
    70561 tgagacgcct gccagttata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt
    70621 ttacaaccgt ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc
    70681 cggtgtaatc atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc
    70741 cgatcagatc atcgtcgcct tacaggatgg acgtgtattt gaagcattgc tggtgggatc
    70801 tgactctcta accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat
    70861 tccgatcaat gcacgtcgcg taccgcacat tggcgacgtg gtactggcga tcggtaaccc
    70921 gtacaacctc gggcagacca ttacccaggg gattattagt gctacgggtc gaatcggtct
    70981 gaacccgacc gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc
    71041 tggcggcgcg ctggtgaact cgctgggaga actgatgggc attaacacgc tgtcgtttga
    71101 taagagtaac gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc
    71161 aaccaaaatt atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggtat
    71221 cggcggacgc gagatcgcac cactgcacgc gcagggcggt ggtatagatc aactgcaagg
    71281 gatcgtggtt aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa
    71341 cgatctgatt atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca
    71401 ggtggcagaa attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca
    71461 gttaacgctg caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa
    71521 acaaaaaacc ggagtctgtg ctccggtttt ttattattcg ctaattaatt acttattaac
    71581 gaactcttcg cccagggcga tatctttctt cagcgtatcc agcatacctt ccagtgcgct
    71641 ctgttcaaat gcgctcaggg taccgataga tttacgctct tccacgccgt ttttacccag
    71701 cagcagcggt tgagagaaga aacgggcgta ctgaccgtcg ccttcaacgt aggcacattc
    71761 gacaacgcct tgttcgccct gcagtgcacg aaccagagac agaccaaaac gtgcagctgc
    71821 ctggcccata gacagggttg cagacccacc accggctttc gcttcaacca cttcagtacc
    71881 tgcgttctgg atacgtttgg tcagatcagc cacttcctgc tcggtaaaac taacgccagg
    71941 aacctgtgac agcagcggca gaatggtaac accagagtga ccgccaataa ccggcacttc
    72001 aacttcgcct ggctgtttgc ctttcagttc cgcaacaaaa gtgttggaac gaatgatatc
    72061 cagcgtggta acgccgaaca gtttgttttt gtcataaaca ccggcttttt tcagcacttc
    72121 agcagcaatc gcaactgtgg tgttaaccgg gttagtgata ataccaatgc acgctttcgg
    72181 gcaggttttc gcaacttgct gtaccaggtt tttcacgatg ccggcgttaa cgttaaacag
    72241 gtcggaacga tccatacccg gtttacgcgc tacacctgca gagataagaa cgacatctgc
    72301 gccttccagc gccggagtcg catcttcacc agaaaaacct ttgattttca cagcagtagg
    72361 gatatggctc agatcgacag ccacaccggg agtcactgga gcgatatcat acagagagag
    72421 ttctgaacct gaaggcagtt gggtttttaa cagtagtgca agcgcctggc caataccgcc
    72481 agcagcgccg aggactgcga ctttcatcct aaactcctta ttatattgat aaactaagat
    72541 atgttgctcc gctgccgcga ccttaatcca caaaattgcc gtttacaatg accacatctc
    72601 aagaatgtgt agtcacgcaa gtttagcgtt tatgcattta attgccgtaa tcaggaacct
    72661 aacttacgta attaacagtc gcttatgcat tagcgcaaca ttccaacagg tggtgacaat
    72721 ataccctacc gttcagccaa aacaacatca atttgataac aattaattta cttttaaaca
    72781 gaatttgcat gccgtgacgc aggcatgttt ctcaataacg aaatttgata aaatcccgct
    72841 ctttcataac attatttcag ccttcttcag ggctgactgt ttgcataaaa attcatctgt
    72901 atgcacaata atgttgtatc aaccaccata tcgggtgact tatgcgaagc tcggctaagc
    72961 aagaagaact agttaaagca tttaaagcat tacttaaaga agagaaattt agctcccagg
    73021 gcgaaatcgt cgccgcgttg caggagcaag gctttgacaa tattaatcag tctaaagtct
    73081 cgcggatgtt gaccaagttt ggtgctgtac gtacacgcaa tgccaaaatg gaaatggttt
    73141 actgcctgcc agctgaactg ggtgtaccaa ctacctccag tccattgaag aatctggtgc
    73201 tggatatcga ctacaacgat gcagttgtcg tgattcatac cagccctggc gcggcgcagt
    73261 taattgctcg cctgctggac tcactgggca aagcagaagg tattctgggc accatcgctg
    73321 gcgatgacac catctttacc acccctgcta acggtttcac cgtcaaagac ctgtacgaag
    73381 caattttaga gctgttcgac caggagcttt aatctctgcc ccgtcgcttc tggcgtcggg
    73441 gaaaatgttg cttatccctc tcattcccct tctttctcct gcgatacatt taacgaatag
    73501 tgcgttttac cgcgacacat cattcacaaa atgaatacat acggtaaaaa aagcacatta
    73561 tgcaaa
//
LOCUS       DAZFKM010000007        69727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFKM010000007 DAZFKM010000000
VERSION     DAZFKM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..571
                     /locus_tag="ACHKAY_000526"
     CDS             53..571
                     /locus_tag="ACHKAY_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5699256.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            587..1126
                     /locus_tag="ACHKAY_000527"
     CDS             587..1126
                     /locus_tag="ACHKAY_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO5699257.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHKAY_000528"
     CDS             complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHKAY_000528"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO5699258.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHKAY_000529"
     CDS             complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHKAY_000529"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO5699259.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4495..5865
                     /gene="xseA"
                     /locus_tag="ACHKAY_000530"
     CDS             4495..5865
                     /gene="xseA"
                     /locus_tag="ACHKAY_000530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO5699260.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5862..6077)
                     /locus_tag="ACHKAY_000531"
     CDS             complement(5862..6077)
                     /locus_tag="ACHKAY_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5699261.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHKAY_000532"
     CDS             complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHKAY_000532"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO5699262.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHKAY_000533"
     CDS             complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHKAY_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO5699263.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHKAY_000534"
     CDS             complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHKAY_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO5699264.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHKAY_000535"
     CDS             complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHKAY_000535"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO5699265.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHKAY_000536"
                     /gene_synonym="gcpE"
     CDS             complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHKAY_000536"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO5699266.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHKAY_000537"
     CDS             complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHKAY_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO5699267.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13391..14545)
                     /locus_tag="ACHKAY_000538"
     CDS             complement(13391..14545)
                     /locus_tag="ACHKAY_000538"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO5699268.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHKAY_000539"
     CDS             complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHKAY_000539"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO5699269.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHKAY_000540"
     CDS             complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHKAY_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO5699270.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHKAY_000541"
     CDS             complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHKAY_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO5699271.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22756..23601
                     /gene="sseA"
                     /locus_tag="ACHKAY_000542"
     CDS             22756..23601
                     /gene="sseA"
                     /locus_tag="ACHKAY_000542"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO5699272.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23937..24062
                     /gene="timP"
                     /locus_tag="ACHKAY_000543"
     CDS             23937..24062
                     /gene="timP"
                     /locus_tag="ACHKAY_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO5699273.1"
                     /translation="MKIRCFCIVLLVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="ACHKAY_000544"
     CDS             complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="ACHKAY_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO5699274.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="ACHKAY_000545"
     CDS             complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="ACHKAY_000545"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO5699275.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26473..26673)
                     /gene="iscX"
                     /locus_tag="ACHKAY_000546"
     CDS             complement(26473..26673)
                     /gene="iscX"
                     /locus_tag="ACHKAY_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO5699276.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26685..27020)
                     /gene="fdx"
                     /locus_tag="ACHKAY_000547"
     CDS             complement(26685..27020)
                     /gene="fdx"
                     /locus_tag="ACHKAY_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO5699277.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27022..28872)
                     /gene="hscA"
                     /locus_tag="ACHKAY_000548"
     CDS             complement(27022..28872)
                     /gene="hscA"
                     /locus_tag="ACHKAY_000548"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO5699278.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28889..29404)
                     /gene="hscB"
                     /locus_tag="ACHKAY_000549"
     CDS             complement(28889..29404)
                     /gene="hscB"
                     /locus_tag="ACHKAY_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO5699279.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29500..29823)
                     /gene="iscA"
                     /locus_tag="ACHKAY_000550"
     CDS             complement(29500..29823)
                     /gene="iscA"
                     /locus_tag="ACHKAY_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO5699280.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29840..30226)
                     /gene="iscU"
                     /locus_tag="ACHKAY_000551"
     CDS             complement(29840..30226)
                     /gene="iscU"
                     /locus_tag="ACHKAY_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO5699281.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30254..31468)
                     /gene="iscS"
                     /locus_tag="ACHKAY_000552"
     CDS             complement(30254..31468)
                     /gene="iscS"
                     /locus_tag="ACHKAY_000552"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO5699282.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31709..32197)
                     /gene="iscR"
                     /locus_tag="ACHKAY_000553"
     CDS             complement(31709..32197)
                     /gene="iscR"
                     /locus_tag="ACHKAY_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO5699283.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32498..33235)
                     /gene="trmJ"
                     /locus_tag="ACHKAY_000554"
     CDS             complement(32498..33235)
                     /gene="trmJ"
                     /locus_tag="ACHKAY_000554"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO5699284.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33354..34157
                     /gene="suhB"
                     /locus_tag="ACHKAY_000555"
     CDS             33354..34157
                     /gene="suhB"
                     /locus_tag="ACHKAY_000555"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO5699285.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34302..35156
                     /locus_tag="ACHKAY_000556"
     CDS             34302..35156
                     /locus_tag="ACHKAY_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5699286.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35347..36627
                     /gene="csiE"
                     /locus_tag="ACHKAY_000557"
     CDS             35347..36627
                     /gene="csiE"
                     /locus_tag="ACHKAY_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO5699287.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36619..37758)
                     /locus_tag="ACHKAY_000558"
     CDS             complement(36619..37758)
                     /locus_tag="ACHKAY_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO5699288.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37918..38808)
                     /gene="hcaR"
                     /locus_tag="ACHKAY_000559"
     CDS             complement(37918..38808)
                     /gene="hcaR"
                     /locus_tag="ACHKAY_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO5699289.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38944..40305
                     /gene="hcaE"
                     /locus_tag="ACHKAY_000560"
     CDS             38944..40305
                     /gene="hcaE"
                     /locus_tag="ACHKAY_000560"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO5699290.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40302..40820
                     /gene="hcaF"
                     /locus_tag="ACHKAY_000561"
     CDS             40302..40820
                     /gene="hcaF"
                     /locus_tag="ACHKAY_000561"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO5699291.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40820..41140
                     /locus_tag="ACHKAY_000562"
     CDS             40820..41140
                     /locus_tag="ACHKAY_000562"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO5699292.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41137..41949
                     /gene="hcaB"
                     /locus_tag="ACHKAY_000563"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41137..41949
                     /gene="hcaB"
                     /locus_tag="ACHKAY_000563"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO5699293.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41959..43161
                     /gene="hcaD"
                     /locus_tag="ACHKAY_000564"
     CDS             41959..43161
                     /gene="hcaD"
                     /locus_tag="ACHKAY_000564"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO5699294.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGTVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43258..43680
                     /locus_tag="ACHKAY_000565"
     CDS             43258..43680
                     /locus_tag="ACHKAY_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5699295.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43728..44600)
                     /locus_tag="ACHKAY_000566"
     CDS             complement(43728..44600)
                     /locus_tag="ACHKAY_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO5699296.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44612..45706)
                     /locus_tag="ACHKAY_000567"
     CDS             complement(44612..45706)
                     /locus_tag="ACHKAY_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO5699297.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45739..46737)
                     /locus_tag="ACHKAY_000568"
     CDS             complement(45739..46737)
                     /locus_tag="ACHKAY_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5699298.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46762..48273)
                     /locus_tag="ACHKAY_000569"
     CDS             complement(46762..48273)
                     /locus_tag="ACHKAY_000569"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5699299.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48296..49279)
                     /locus_tag="ACHKAY_000570"
     CDS             complement(48296..49279)
                     /locus_tag="ACHKAY_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5699300.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49376..52657)
                     /locus_tag="ACHKAY_000571"
     CDS             complement(49376..52657)
                     /locus_tag="ACHKAY_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO5699301.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52775..53968
                     /locus_tag="ACHKAY_000572"
     CDS             52775..53968
                     /locus_tag="ACHKAY_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO5699302.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54032..55285)
                     /gene="glyA"
                     /locus_tag="ACHKAY_000573"
     CDS             complement(54032..55285)
                     /gene="glyA"
                     /locus_tag="ACHKAY_000573"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO5699303.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55613..56803
                     /gene="hmpA"
                     /locus_tag="ACHKAY_000574"
     CDS             55613..56803
                     /gene="hmpA"
                     /locus_tag="ACHKAY_000574"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO5699304.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56848..57186)
                     /gene="glnB"
                     /locus_tag="ACHKAY_000575"
     CDS             complement(56848..57186)
                     /gene="glnB"
                     /locus_tag="ACHKAY_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO5699305.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57247..58581)
                     /gene="glrR"
                     /locus_tag="ACHKAY_000576"
     CDS             complement(57247..58581)
                     /gene="glrR"
                     /locus_tag="ACHKAY_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO5699306.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58571..59284)
                     /gene="qseG"
                     /locus_tag="ACHKAY_000577"
     CDS             complement(58571..59284)
                     /gene="qseG"
                     /locus_tag="ACHKAY_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO5699307.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59449..60876)
                     /gene="qseE"
                     /locus_tag="ACHKAY_000578"
     CDS             complement(59449..60876)
                     /gene="qseE"
                     /locus_tag="ACHKAY_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO5699308.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61452..65339)
                     /gene="purL"
                     /locus_tag="ACHKAY_000579"
                     /gene_synonym="purI"
     CDS             complement(61452..65339)
                     /gene="purL"
                     /locus_tag="ACHKAY_000579"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO5699309.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65597..67153
                     /gene="mltF"
                     /locus_tag="ACHKAY_000580"
     CDS             65597..67153
                     /gene="mltF"
                     /locus_tag="ACHKAY_000580"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO5699310.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67150..67653)
                     /gene="tadA"
                     /locus_tag="ACHKAY_000581"
     CDS             complement(67150..67653)
                     /gene="tadA"
                     /locus_tag="ACHKAY_000581"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO5699311.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67711..68346)
                     /gene="yfhb"
                     /locus_tag="ACHKAY_000582"
     CDS             complement(67711..68346)
                     /gene="yfhb"
                     /locus_tag="ACHKAY_000582"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO5699312.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68555..69403
                     /locus_tag="ACHKAY_000583"
     CDS             68555..69403
                     /locus_tag="ACHKAY_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO5699313.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69459..69719
                     /gene="yfhL"
                     /locus_tag="ACHKAY_000584"
     CDS             69459..69719
                     /gene="yfhL"
                     /locus_tag="ACHKAY_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO5699314.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
BASE COUNT        16535 a        19245 c        17597 g        16350 t
ORIGIN      
        1 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
       61 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
      121 gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
      181 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaactc
      241 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt
      301 tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg cagttggcgg
      361 cggagctgta ggcgcggcag tgggttctat ggtgaatgat aaaaccttag tggaaggtgt
      421 ttctttaaca tataaggaag gcaccaaagt gtatacctct acccaggtgg gtaaagagtg
      481 ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa cgcgtattca
      541 gccaaatact aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
      601 tctttgcgcc atcttggcct ccttaagcta tccggctatc gcctcatcat tgcaggatca
      661 actctctgct gtagcagaag ctgaacagca aggtaaaaat gaagagcaaa ggcagcatga
      721 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
      781 ccaggccgcc gctaacaaaa gagcggcaac ggcagcggca aataagaaag ctcgtcagga
      841 taaactggac gccgaagcct ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      901 acgcagctta gagattcaga aacaaaaact ggcgctggca aaagaagaag cccgcgttaa
      961 gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
     1021 gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc tgatgaaacg
     1081 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
     1141 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacagaggg ttttgttcat
     1201 tcatagtgca gggattaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1261 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1321 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1381 cgcagagaga caacccaatc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1441 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1501 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1561 agcacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1621 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1681 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1741 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
     1801 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1861 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1921 cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa catatccaga
     1981 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     2041 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2101 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     2161 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2221 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2281 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2341 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2401 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2461 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2521 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2581 tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg ggccgccgga aagaataatg
     2641 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2701 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2761 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2821 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2881 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2941 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     3001 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3061 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3121 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3181 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3241 ccagcatgga acctaccatc accgcgcttg cgccagctgc gatagctttg gcgatgtcgc
     3301 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3361 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3421 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3481 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3541 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3601 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3661 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3721 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3781 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3841 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3901 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3961 taacgaccgg atagcccgca aaaccgttac gctcggtcag ttctttcact tcgcgcagcg
     4021 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4081 gacgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4141 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4201 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4261 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4321 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4381 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4441 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4501 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4561 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4621 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4681 ttccgcaaca gcaaccgtcg ggtgaccttc cgtccacagc atgggcaaca agttttagtt
     4741 cgcgccaata ttacgctcta cgagccgcgc ggcgactacc agataatcgt tgagagtatg
     4801 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4861 gctgaaggat tgttcgatct gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4921 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4981 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcgccg
     5041 gggcaaactg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5101 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5161 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5221 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5281 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5341 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     5401 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5461 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5521 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5581 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     5641 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5701 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5761 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
     5821 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5881 acgcgttttt tcgatatcaa accatgcccg tgctgacaga agtaatctac tgcaccgcag
     5941 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     6001 tccccgcagg atgggcaacg cgcatggtcg ttatcctgat caagcacatg ctgacattgt
     6061 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     6121 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6181 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6241 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6301 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6361 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     6421 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6481 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6541 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6601 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6661 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6721 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcatct tcaatggcct
     6781 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6841 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6901 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6961 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     7021 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7081 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7141 gcagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7201 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7261 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7321 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7381 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7441 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7501 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7561 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7621 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7681 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7741 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7801 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7861 cgacgttaat ccagtgcaga taaccttcac tgtcacctac caccaggttg ccattataca
     7921 gcaccggaga agtcagcagg cgatgcaaca gatcgctttg tgtccacagc gtaacgccgc
     7981 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     8041 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8101 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     8161 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8221 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8281 cgccacccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8341 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcctcg ttcagcgctt
     8401 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8461 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8521 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc agaccgccag
     8581 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8641 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8701 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8761 cggaagtgct ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8821 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8881 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8941 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     9001 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9061 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9121 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9181 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9241 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9301 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9361 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9421 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9481 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9541 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9601 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9661 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9721 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9781 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9841 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9901 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt
     9961 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
    10021 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
    10081 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10141 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10201 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10261 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10321 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10381 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10441 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10501 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10561 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10621 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10681 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10741 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10801 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10861 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcta tgcgggttca atcatacacg
    10921 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10981 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    11041 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    11101 gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    11161 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    11221 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt tgatcccgcg
    11281 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11341 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11401 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    11461 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11521 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11581 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11641 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11701 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11761 gacgccgtat tccgctactt tcagtgcaat gcgatagtcg aagtggatgt cagccaccag
    11821 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11881 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11941 ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    12001 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12061 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12121 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12181 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtatgg tgcctggccg
    12241 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12301 tcagtaacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12361 gccggcgtag tcacgcctgc ctgatcggtt ggcaacggcg cagcgccatc tggcgttgtt
    12421 gttgccgttg gtgccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12481 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12541 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12601 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12661 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12721 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtca
    12781 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatag gcgcaacttt tgcagcccgt
    12841 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12901 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12961 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    13021 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13081 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13141 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13201 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13261 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13321 attgcaactt aatacttcat tcataaaaga ttacgccacg gtacataaag taaccgtggc
    13381 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13441 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13501 caccacgggt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13561 ggtcgatacg gctgttcgag ctgcgtccat acggcgcgcc cgggaagggg ttccacggga
    13621 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13681 tgccgtcgtt aacgtggtca agcatcacat actcaatagt gacacggccc tgattggcgt
    13741 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13801 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13861 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13921 tgacgcgacg tttagacagg ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13981 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    14041 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccaa
    14101 taatttccga tacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14161 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    14221 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    14281 atgaacgctg ttcttcaacc acttccggtg cgcggatttc cgccacctct ttcagtttgc
    14341 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    14401 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14461 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    14521 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14581 cctggaggat tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14641 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14701 ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg atttcgcgag cggcagattc
    14761 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14821 agtaccagcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14881 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14941 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgtgcct gttcaacggt
    15001 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15061 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    15121 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15181 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    15241 aatgagtttt agcaacaatc agggacaaaa cagactattg catgacaaat ttcactgtcg
    15301 cgatttgccc cacatcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    15361 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    15421 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    15481 taatcgcgcc atcgcgaacg cctgtcagct gcagagggag ctgggcatcg tgaccgtatg
    15541 acgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    15601 agggttccag cggtagcggc cagacattta tcatttcttg tcgcgcttgc gggcaatcag
    15661 cggcaacacg tttgccattt tcatccagcc agatggggaa acgaatgcca ttaatgcctt
    15721 cctgctccgg cagtaataga gttggcggct gactcccgtc cagcagccag gttgccaggc
    15781 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15841 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15901 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15961 gcgtgccgtc cggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    16021 cccaggcgtc acgatagcca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    16081 gcaaggcact atccggcaag ggtttcgctt catcagccat aatccggcga atgatccacg
    16141 ccgcccccga cgacattaaa ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt
    16201 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    16261 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc accgttgggc aaatataacg
    16321 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    16381 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    16441 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    16501 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    16561 ccgatcctgg cgatcggttc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    16621 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16681 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16741 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16801 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16861 cttcccttga ctcttttacc tgctcacggg accacgcacc ttgcacggcc atccgttcga
    16921 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcga agacggctgg
    16981 gcgcttgcgg caaaaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgact
    17041 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    17101 ggttaagata caaggtcaga atttcacgct tagacagatg ccattccagt tgcaacgcgc
    17161 gccagagctg gcgaattttg ccgccaaatg ttttggggtg aggatcaagc agacgagcaa
    17221 cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg
    17281 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag cgatcttcat
    17341 aattgatcag cgcttcaagg taacgtggag aaacatcttc gattgttacc ggataacgcc
    17401 agatgccgtc agcatcggcg aagcgccata gcggcgtacc atcctgcgcc acgaccactc
    17461 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    17521 gaaaaaggag aaatggcgca gctgccagcg ttatccagca gccgcgtttg gttaacaagc
    17581 gaggcattta cggtcggaca atcagcagat cttcagccgc gccggtcgcc cgccattggg
    17641 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17701 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17761 gatcgtcacg gaactcaatg tgcttaatac tcgcctgctg catctggttc agtaagttct
    17821 gcacttcgcc accactttgc tccaggctgg cgctaccgtt cgccagattc tggttttcca
    17881 gttccagacc cgcaggcagc agatccacga ccaacgcatc cggcacgctg ttactggctt
    17941 ttacctgcaa ccacaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    18001 taccatcagt accaagaata tgacgctcga tttgcagcac atcgttcgcc ggtaaaggtg
    18061 cggattgcgg ataaccgctg gcatccacac gcagccataa cggctgatcg ccactgtttc
    18121 tcacctgcaa ggtggcaagt tgatcgctat tcagattgct gtttagcgtt ttctcgcctg
    18181 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    18241 tacgggcagc caggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaatg
    18301 cctgctgcga aagcgtgttc agcaaagtgt tctgctcatc gggtagcagt ttattttctt
    18361 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    18421 tccgctcatc actatttctc ggcgttttca gcgccagcac aatcgcttct tcaccacgcg
    18481 ttgcatcacc catggttttc agcgcaacgc caagttgcag cagcggtaaa ccagaagcgg
    18541 catctgcacg atgctcccag atttcacgca gcgcacccag tggtgctttt tgctgacggg
    18601 ccagcaccag cgcagcgtaa gactgaacgg cgaatttact ggctttgaga ttatccgcgt
    18661 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18721 taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18781 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18841 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18901 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18961 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    19021 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    19081 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    19141 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    19201 tatgctgatc ggcaacggtt tcacccggta acgctaaccc gctaatggtg gcctgaattt
    19261 ctccatcgcc ataacccggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    19321 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    19381 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    19441 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    19501 ctatcacttt gctttcgttg ctaccgaaat catctgccgt ccaggcttgc gccatgacgc
    19561 gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    19621 gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg attgaccggc ggtttaccac
    19681 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19741 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19801 cctgccacgg atctggcgtg acgtagtcag taatattcag cacaccacta tcaacggcag
    19861 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19921 cggttaatgg ttgattggga cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19981 ggttttcatc gccaagcggc agatgcaaca caccaaccgc gcgttttggc gtcgcggagc
    20041 gagatttatc gccaggacgt accaccagcg tacttaaata cagatcatga cgattccagg
    20101 ttttatcgac cggaatcgtc agatccagcc cttgagcccg aacatcaatc tcttgccacc
    20161 acagcggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcga
    20221 tatgcaactt aatggtgtcg ccagggcgat aactggcttt atccagtttc agcgtgacac
    20281 ggtcgggtcg cactgcgccg ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga
    20341 aacgaacact actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    20401 ctaccggaaa actgaccttg ccggtttcgt ccgctttcag atccagagtt tgttcatttt
    20461 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    20521 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    20581 cttgcgcatc gctataaacg atgtcaaaag cggcgttact gccttcatca acaatgggct
    20641 gtttcaccgt gctgtcagta cgataatcgt aaaccgattt cgaggcgaac tgcggacgga
    20701 tccccggcaa tgcatcggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20761 cgcccgattc cagcaaacta ccctggaaaa taacctgtaa tggggaatgc gtttccttcc
    20821 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20881 ccagcgtgcg ggaaagattt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20941 acacagcttc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    21001 gtgcaccata caggtagtac cccaccacgg agaatttcac ttcatctttc ggcgttagcg
    21061 gggttttctc accggtcaga ttcagcgcca tgcgctctgg cataaaatct tcgacgtgga
    21121 aatcccacat ccgatactgg ttatcgcccg tgttagcgcg aatatgccac ataccggttg
    21181 ccgcattgct atcgagtggc caggtaaagt ggtagaggcc attctccggc tgactaacga
    21241 cgctcctgag tacctgccca tcgggtttaa tcacgtctaa cttgatgggt tgattgggca
    21301 acgctttacc gtctgcatca cgcagcaaac cattgaggat taccgtttca cttgggcgat
    21361 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    21421 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    21481 tacgcgccaa cagtaatgcc gcgtttttat cattttccag ctgcacatgc ccctgagcgt
    21541 cactggttgc ctgagtcaga gtctgccctt tctcatttaa taaagagact tcaattcctt
    21601 gctgggccgc gccgttttcc agactttggg taaagatatc cagacgattg tgataacggt
    21661 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21721 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21781 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21841 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21901 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21961 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    22021 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    22081 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    22141 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtaa ctctttaaga ttatctgaca
    22201 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    22261 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    22321 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    22381 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    22441 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    22501 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct
    22561 gccacaatgg cccgttagca acgtcgaata attattatgc gtgagaattt acgttatgta
    22621 attcagtatc acgcccatac aatccgaatt ttagtgagaa ttgtggctca gacagccaaa
    22681 tcagcctctt tcgcttgtcg cttttcacta aacatccgac aatttagcct aaccccggca
    22741 aaaatggaga tgcctatgtc cacgacatgg tttgtaggag ccgactggct cgccgaacat
    22801 attgatgacc cggaaattca gattattgat gcccgcatgg cgtcgcctgg acaggaagat
    22861 cgtaacgttg ctcaggagta tctgaatgga catattcccg gcgcagtgtt ttttgatatc
    22921 gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc
    22981 gccgtggcga tgcgtgaatt aggcgttaac caggataagc acctgattgt ctatgacgaa
    23041 ggtaatcttt tctcagcccc acgagcatgg tggatgctgc gcacctttgg tgtagagaaa
    23101 gtgtcgattc tggggggtgg acttgcaggc tggcagcgcg atgatctgct gttagaagaa
    23161 ggtgcagtag agctgccgga aggagagttt aacgccgcgt ttaatcctga agccgtggtg
    23221 aaagtaaccg atgtattatt ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc
    23281 ccggctgcac gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat
    23341 attcccggag cactgaatgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg
    23401 accgatgaac tggatgcgat attttttggt cgcggcgtca gctacgacaa accaattatc
    23461 gtcagctgcg gctctggtgt aacggcagcc gtggttttgt tagcactcgc gacgctggat
    23521 gtgccaaacg tgaaactgta cgacggcgca tggagtgaat ggggcgcgcg ggcagattta
    23581 ccggttgagc cagtgaaata agtattttac aggcaataaa aaaccgccga atttggcggt
    23641 tttttattgc tggtctggtt cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa
    23701 gcgcgtctca gcggagacaa tactcgccag taactctctt tttgtcaagc aaaagagagt
    23761 tattattgtt ctgttagtgt attatccact gcggcccttt ccgccgtctc gcaagcgggc
    23821 gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa gcgcgtctca gcgcttgcta
    23881 tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga
    23941 agatacggtg cttctgtatc gtacttcttg tttctggtgc gctgttttcc caagtaaata
    24001 ataaccggag tctctccggc gacaatttac tggtggttaa caaccttcag agcagcaagt
    24061 aagcccgaat gccgcccttt agggcggcat attttagatt atccggttct gtttaaagtc
    24121 acgcaaaaaa ccaccccagc gacgttcata gaatggcgca atatgttcgg taataaagtg
    24181 gctaattcct ttttcccctt ttttcacctg acaaatatcg attggttcat cgccaggtaa
    24241 tgtatcggtc gctacacttc ccgtcgcctg aataatttct tcgatatcac catcggcttc
    24301 aatgccaata agtaaattag gctgtgcctc ttcgttctct ttaattgaac aaataaaagc
    24361 acgcttcacc ggcttaatgg ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg
    24421 cggctctgcg acttccgata atatcagcga ttcaccgcct tccaggattt cctggctgct
    24481 cagcggattt ccctctccac caatcaacaa actgatttca cgcggcataa attctttacc
    24541 ggtcggcagt ttggcattaa ggaagagcgt ttcgccaagg gtcatctcaa acaatgtgcg
    24601 aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc tgttgaagcg cttctaacga
    24661 ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc
    24721 actatcttca accacagcct cgccctgcgc cgccgtacca ggcacccaga cggtggattc
    24781 cagtagagta cggaaaaagc ccgggcggtg cgccggttcg gttgctgctt tttccagcag
    24841 gtcttcaagt tcgttttttg tttcggacat atgaaccaca attcattcaa cgttcggggc
    24901 gcaaatgcct gatgcgctac gcttatcagg cctacaaggg attcgcaatt tgttgaattt
    24961 gcagaatttg taggccggat aaggcgttta caccgcatcc ggcatattag tttacgccgt
    25021 taacagatta gctatcgtgc gcacaccaag tcccgtagcg cccgcagacc actgttcaac
    25081 cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc
    25141 aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct gccggatacg ccgcgcttcc
    25201 ggtattgttc agttcggcaa agttagacgg cagctggctg cggtggaatt ccgccagcgg
    25261 cagacgccag aatggttcgt tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc
    25321 atcgtcaaaa ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt
    25381 gagggtcgcc gcatcaatga tcatttccgg tttctgcgca ctggcatcaa tcagaccatc
    25441 ggcaagcacc agacgccctt cggcatcagt gttcatcact tcaacttttt taccgttgcg
    25501 ataggtgatg atatcgccca gcttgaacgc attgccgcta atcaggttat ccgcacagca
    25561 gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt
    25621 aaccgttgcc gcgccgccca tgtccgactt catcgagtcc ataaacgcag tctgtttgat
    25681 gctgtagccg ccggagtcaa aagtgatacc tttacctacc aggcacgcgt acaccggtgc
    25741 ttctttatcg ccagttgggt tgtaatccag cgccagcaat accggagaac gttctgaacc
    25801 gcgcccgacg gtgtgcagcc ccatataacc ttgctcacgc agatcttcgc ctttggtgat
    25861 ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga tcaacagcac gctgtgccag
    25921 ttgcgatggt cccaattctt ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat
    25981 catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc agatccggcc actccacttt
    26041 acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca
    26101 gccttcaccg cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg
    26161 ctggatcagc cccagatcgt ctgccccgtt caaatgcagg gtaatgccgt cattattaat
    26221 gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt
    26281 catcgcttct gtcattttag ttatccttct ttgtaaaagg gccgccagtt ggcagcccca
    26341 ggatttttcc gctaaatgat tcgcgttgca ggaaggcggc aagtgagtga agccccagga
    26401 gcatagataa ctatgtgact ggggtgaacg agcgcagcca acgcatctgc ggcgtgaagc
    26461 atgacgcgga aattattcgg cctcgtccag ccagactaac aaaatcgctt cgaggatttt
    26521 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
    26581 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
    26641 aatttcgcgg ctatcggtcc atttaagtcc catactaacc tctgttaatg ctcacgcgca
    26701 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
    26761 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
    26821 tctgagcttt ccggcagcga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
    26881 gcacaggatt tttcacaggc gtgttcaatc tcgataccgt tacgcagagc tgcgtcgaga
    26941 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagagatc ctgatgaggc
    27001 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
    27061 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
    27121 cgcttgttcg atggcgtcaa cgtcatcgcc ctgcgccact tcactcaggt gagcggcagc
    27181 atcgtcaatg acctgacgtt ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
    27241 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca ttcgggcttt
    27301 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
    27361 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
    27421 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
    27481 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
    27541 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
    27601 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
    27661 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca acagcatttc
    27721 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
    27781 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
    27841 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
    27901 tacacccgcg tctttaagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
    27961 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
    28021 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gcggcatcca gcagttcacg
    28081 ctgaacgcgg ttatcgctac gatcaggaat gcccgcctgc tcgcgaatgt aatccgccag
    28141 cagatggtcg aaatcatcac caccgagcgc ggaatcaccg ccggtcgcca gcacttcaaa
    28201 cacaccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccgccga ggtcataaac
    28261 ggcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cggtcggttc
    28321 gttaagtaag cgcaggacgt gaaggcccgc cagacgcgcc gcgtctttgg tgccctgacg
    28381 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
    28441 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
    28501 cagcagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
    28561 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
    28621 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
    28681 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
    28741 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
    28801 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
    28861 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
    28921 cggcactgct ccgcagttta tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt
    28981 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
    29041 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
    29101 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
    29161 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
    29221 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
    29281 cctggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
    29341 ggctcagcgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
    29401 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
    29461 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
    29521 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
    29581 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
    29641 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
    29701 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
    29761 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
    29821 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
    29881 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
    29941 cttcagcaat atcggtattt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
    30001 attcggtgac cagggagctg gaagcgatag cagaaccgca gccgtaagtt ttaaaacgcg
    30061 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcatcacg tcgccacagg
    30121 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt
    30181 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
    30241 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
    30301 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
    30361 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
    30421 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
    30481 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
    30541 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
    30601 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
    30661 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcctcg cccattccga
    30721 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
    30781 tttgcgcttc gatgcgtacg cgcggtttac gacgtacata cagcgcaccg atacctttcg
    30841 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
    30901 taggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
    30961 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
    31021 tggaaacaag gatggtgtcg tcacgcatcg ctgcttcaag ttctttcagg tcgataatgc
    31081 cgttacgctg cggtgccagg taggtgactt caaaaccttc gcgctccagc tgacggcagg
    31141 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
    31201 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
    31261 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
    31321 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
    31381 ccatcgtcat aaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
    31441 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
    31501 aggaaccagg ctaccggctg gatgtacgac cgtgtttacg gagcgcgata cgccccggcc
    31561 tgattctaat atcttgttat ctttaaccta actgtttcgg gtttcagcaa gttgacaaac
    31621 ttacgaatct ccgggagctt acataagtaa gtgaccggag tgagtatgtg cagtcaacgc
    31681 agatgcaaca cgaaacagga cggttaaatt aagcgcgtaa cttaacgtcg atcgcgtctt
    31741 gtatgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct
    31801 ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca
    31861 aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct
    31921 gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc
    31981 tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac
    32041 catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt
    32101 cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg
    32161 cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc
    32221 ccctgcccgg ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac
    32281 attcccgagt aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca
    32341 gtgccatttt tttatcgtgg cgtttgtacc tgttgtcctt ccagaccatg ggcgggttga
    32401 gggcaattat tcgcattagc gctggcctct tcccctcacc ctaaccctct ccccgaaggg
    32461 gcgaggggac cgtccgtgca tttatctggc ttagccctca ggccttatta cctttattct
    32521 gctgctcaat agaagccaga atcccgcgca ggatattcaa ttcctggctt tccgggcgcg
    32581 cacgggtaaa cagacggcgc aatttattca tcacctgccc cggatggttt tcacggataa
    32641 aaccggttgc cagcagcgtt tgttccagat gaccataaaa acgctccaga tcatcgacca
    32701 gcggatacgg cgtctcttca tgctcgacct gctcgccgtt ttcctgagtt gccagccagg
    32761 ccatgcgcac ttcataggca ataacctgag ccgccatcgc caggttcagc gagctgtatt
    32821 ccgggttagc cgcaatcgcg acatgataat ggcatttctg caactcttca ttggtcaagc
    32881 cgacgcgctc gcgaccaaac accagcgcca ccggggtatt tgccgcttca gcgacgcttt
    32941 tcaggccgca ttcgcgcggg tcgagcatcg gccatggcag cgtgcgggag cgtgcgctgg
    33001 tgcccaccac caggctacaa ccggctaacg cttcgtcgag cgtatcgacg atgtgagcat
    33061 taccaatcac atcgctggcc cctgctgcca gggcaatcgc ctgggagtcg ggtttcacca
    33121 gtggattaac cagccacaga ttggttaatc ccattgtttt catggcacgg gcaacagaac
    33181 ccatattgcc ggtgtgtgac gtctccacca gcacaattcg aatattttgc agcattgtct
    33241 ttcttcatct aaagattatt cacgcatctt atcataaaac gaagacagat gccgatctcg
    33301 ctgctatact ctgcgccgtt ttcccgttct ttaacatcca gtgagagaga ccgatgcatc
    33361 cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg taatttaatt gccaaaaact
    33421 atgaaacccc ggacgctgta gaagcgagcc agaaaggcag taacgatttc gtgaccaacg
    33481 tagataaagc tgccgaagcg gtgattatcg acacgattcg taaatcttac ccacagcaca
    33541 ccatcatcac cgaagaaagc ggtgaacttg aaggtactga tcaggatgtt caatgggtta
    33601 tcgatccact ggatggtact accaacttta tcaaacgtct gccgcacttc gcggtatcta
    33661 tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt atacgatcct atgcgtaacg
    33721 aactgttcac cgccactcgc ggtcagggcg cacagctgaa cggctaccgt ctgcgcggta
    33781 gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg cttcccgttc aaagcaaaac
    33841 agtacgccac tacctacatc aacatcgtcg gcaaactgtt caacgaatgt gcagacttcc
    33901 gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc tgcgggtcgt gttgacggtt
    33961 tctttgaaat cggtctgcgt ccgtgggatt tcgcggcagg cgagctgctg gttcgtgaag
    34021 cgggcggcat cgtcagcgac ttcaccggtg gtcataacta tatgctgacc ggtaacatcg
    34081 ttgctggtaa cccacgtgtt gtgaaagcca tgctggcgaa catgcgtgac gagttaagcg
    34141 acgctctgaa gcgttaatga ctcaggcggg tgatgtcact cacccgccct cgcctttcag
    34201 gcgctattct gaaatacttc ctcaccgctt tactttcttt cgtcactctc ccaccatctt
    34261 tccccgatta atggatgata aagaagtcgc aaggaaaggt tatggcactg ccagtgaaca
    34321 aacgcgttcc caaaattctg tttattctct ttgttgttgc cttctgcgtt tatttagtgc
    34381 cgcgcgttgc catcaacttc ttctattatc ctgacgataa aatttacggt cccgacccct
    34441 ggtcggcgga atccgtcgaa tttacggcta aggacggtac tcgtctgcaa ggctggttta
    34501 ttccttcttc gacgggccct gctgacaacg ccatcgcaac catcattcat gctcacggca
    34561 atgccggaaa tatgtccgcc cactggccgc tggtcagttg gttacccgag cgtaatttca
    34621 acgtttttat gtttgattat cgcgggtttg gtaaatcaaa aggcacgccg tcccaggccg
    34681 gattgctgga cgatacgcaa agcgccatca atgtggtgcg ccatcgcagt gatgtaaacc
    34741 cacaacggct ggtgctgttc gggcagagta ttggcggggc gaatattctt gctgttattg
    34801 gtcagggtga tcgtgaaggc atacgtgcgg tgatcctcga ctccacattt gcctcttatg
    34861 caaccatcgc caaccagatg atccccggca gtggctactt acttgatgag agttacagcg
    34921 gcgaaaatta tatcgccagc gtcagcccga tcccgctttt actcattcac ggtaaagctg
    34981 atcacgttat cccatggcag cacagcgaaa agttgtatag cctggcaaaa gagccaaaac
    35041 ggttgatcct aatcccggat ggcgaacaca ttgatgcttt ttccgatcgc cacggcgatg
    35101 tttatcgcga acagatggtg gactttatcc ttagcgcgtt gaatccgcag aactaaccca
    35161 tgatcgctag cacgataatc attcacaaaa ccaccttaag acatgctaat ccactggtca
    35221 gaacagttta agatgagaaa aattctgtga cgcttgccaa catttctgat gattagcatt
    35281 cccttcgcca tttccctgag caaactttag ctattcttat caattatgct tatgggagat
    35341 ctacagatga tgcctacgct tgctccacca tctgtccttt cggctcccca gcgccgctgc
    35401 cagatcttgc tgacgctctt tcagccgggg ttgaccgcca ccatggcaac cttcagcgag
    35461 cttaatggtg tggatgatga tattgccagt cttgatatca gcgaaacagg acgggagatc
    35521 ctgcgctatc atcaactcac actgacgact ggttatgacg gtagctaccg ggttgaaggt
    35581 acagtgctta accaacgttt gtgtttattt cactggctac gacgtggttt ccgtctgtgt
    35641 ccgtcattta ttaccagcca attcaccccc gccctgaaga gtgaactgaa gcggcgcgga
    35701 attgcgcgta acttttacga cgataccaat ctacaagcgt tagtgaatct ctgctcccga
    35761 cggctgcaaa aacgctttga atcgcgcgat attcatttcc tgtgtctgta tctgcaatat
    35821 tgtttgctgc agcaccacgc tggaattacg cctcagttta atccgctcca acgtcgctgg
    35881 gcggaatcct gccttgaatt tcaggtagcg caggaaattg gacgccactg gcagcgtcgg
    35941 gcgctccagc ctgtaccgcc tgatgagcca ctgtttatgg cactactttt ttccatgttg
    36001 cgggttcccg atccactgcg ggatgcgcat cagcgagaca gacaattgcg tcagtctatc
    36061 aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc gtttttatga tgaacagggg
    36121 ttatgtgatc agctttatac ccacctcgcc caggcgttaa atcgcagttt gtttgccatc
    36181 ggtattgata ataccctgcc ggaagagttc gccagactgt acccacgcct ggtgcgcacc
    36241 acccgcgcgg cgctggccgg atttgaaagt gaatacggcg tccatctttc tgatgaggaa
    36301 agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc aggaaaacga tctgcatgaa
    36361 aaacagatta ttctactgac cgggaatgat agcgagcgag aagcgcagat tgagcagcag
    36421 ttacgcgaac taacgttact gccgctcaac attaagcata tgtcggtaaa ggcatttttg
    36481 cagacaggcg ctccgcgtgg cgcggcactg attattgcgc cttataccat gccattaccg
    36541 ctcttttcac caccgctgat ctatacggac ctgacgctga caacacatca acaggagcag
    36601 atccgcaaaa tgctcgaatc agcatgaggg aacaactttc gggcgcaaaa acattgccgg
    36661 cagcgccacc agcgccatta cccagaacac gccgtggccc agatattgat acaggaaacc
    36721 ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg acggcagagt aaaccgcctg
    36781 taaacggatg acttcgctac cctggcgagc agcaatataa cgcatggcgg ccaggtggca
    36841 gaccgtgaag gtgccgcaat gcagaatttg caccactatc aaccacggca acgccgtagt
    36901 tgctcccata atgccccagc gcactacgcc gcaaatcgcc gagatcaaca gcatatcgcg
    36961 tgcactacaa cggcggaaaa gtttattact cagcgcaaag ataatgactt ccgccaccac
    37021 gcccagcgac cacaaatacc ccaccgccga ggccgagtag ccagctgcct gccagtaaat
    37081 ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat aaacaaacgc aggccagaaa
    37141 gcgccagttc tggcgaacca gcgccaacca cgcagaccaa ccggtgctct cctgctggcg
    37201 acttgcccct tgtggctgaa tcgtcggacg gatgagaaag ccgagcagca tggatgccac
    37261 gcccaacgtc aacagcgcga ggatcacccg ataatcaaac atagtgacca gtttgcccgt
    37321 cagcgccgag ccaatgacaa acgccaccga gccccacagt cgcactttgc cgtaatcaag
    37381 cgggaactgc ttttgccacg tattcgccag tgcatcggtc aacggtacca gtggtgagaa
    37441 aaagaggtta aagccaatca tcaccagcat cagccacgct acgtgcgccc ccgcccagaa
    37501 ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc aaggcggaaa tcaggcggga
    37561 aggatcgctg acgcggggcg cgatgagcaa actcccgagg aaacgggcaa ccagacctgc
    37621 ccccaataac aggccgatgg tttctggcgt taaaccaatc cctttaagcc agacgctcca
    37681 gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa tagccgagcg ccaaccagcg
    37741 cgtggattgc aaaaccatga gtccctcccg tcaggaggcg ttagtctggg gttaatcgaa
    37801 tgttggtgca agttgaaaaa taagaatatt cgagcctgct tcccgatgtc tgtaacgcca
    37861 gttcacaagc actaaaaaac tgagatccgc gacgcatgtt gcaaaaataa tgcgtaatta
    37921 tgccgttacg cttgccaaac gttcctgcac aatggcgatg aaatcgcgta atgccggctt
    37981 catctcccct ttcttccacg ccatcagtaa agcaatggaa ggcacatttc cggcaatggg
    38041 acgaaaaaca acctgtccgg tattaaaatt attcatataa ccgggtatca aagtgacgcc
    38101 cagccccatg cccaccagat tcatggtcac cagaatattc gttgccacct ggacgatatt
    38161 tggctggctg ttttcttgcg caaaccacgc tttaacgatc ggcgcaagcg aaccggaata
    38221 caccggatcg gtactgacga aattcacgcc atccagttgc gcggcggtga tctctttttc
    38281 atgtgctaaa gggtgatcaa ccggtaacac aaccactaat ggttcgtcaa aaagctccag
    38341 ataatcaatc tccgggctat aaacaggatg gcgcattaag ccgacgtcga gttcaccacg
    38401 gcgaattttt tcctcctgtt gcgtggtgat taaactcacc agctcaatca aggtgtctgg
    38461 ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa ttcacttccg ccgatggcac
    38521 aaagccaatg gttaattgtc tgtcttcctg aacaattttc cgcgcccgta atttggcatt
    38581 ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa cattctcccg ccgccgtcaa
    38641 cgcgactttg cgcttatccc tcaccagtaa cggaacaccg acacagtttt caagatcgcg
    38701 gatctggctg cttaacgaag gctgtgaggt atgcagtttt tccgccgcac gggtaaagtt
    38761 cagtgcctgc gccactgcga cgaaatagcg taaatgccgt agttccatca ccttcccctt
    38821 gttatcgaaa aaacgtctca gctggtagaa attaactatt tcacatatta gcaaccaacc
    38881 agcaacatcc ttatggcaca aaaatagaag gtcaatacat cttatctttc aggattaaaa
    38941 aatatgacca caccctcaga tttgaacatt taccaactga ttgataccca aaatggtcgg
    39001 gtcactccgc gtatttatac cgacccagat atttaccaac tggagcttga acgtattttc
    39061 ggccgttgct ggttatttct cgcccacgaa agccagatcc caaaacctgg tgatttcttt
    39121 aacacctaca tgggggaaga tgcggttgtc gtagtgcgtc agaaagacgg cagcattaag
    39181 gcgtttctca accaatgccg ccaccgggcc atgcgtgtga gttatgcaga ttgcggcaac
    39241 actcgcgcct ttacctgtcc gtatcacggc tggtcttatg gcattaacgg cgagttgatc
    39301 gatgtaccgc tggaacctcg cgcctaccca caagggttgt gtaaatccca ctggggacta
    39361 aacgaagttc cttgtgtgga gagttataaa gggctgattt ttggcaactg ggataccagc
    39421 gcaccgggcc tgcgtgatta cctgggtgac attgcctggt atctggatgg catgctggat
    39481 cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa agtgggtgat caactgtaac
    39541 tggaaattcc cggcagagca gttcgccagt gaccagtatc atgctctgtt cagccatgct
    39601 tctgccgttc aggtattagg ggcgaaagat gatggcagcg ataagcgcct cggtgatgga
    39661 caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc tgcaatttgg tcaggacggt
    39721 cacggtagcg gtttcttctt tactgaaaaa ccggatgcta atgtctgggt cgatggcgca
    39781 gtttcaagct attaccgcga aacctatgcc gaagcagaac aacgtttagg tgaagttcgc
    39841 gccctgcgcc tggcgggtca taacaatata ttccccacgc tttcatggct aaacggcact
    39901 gccacactcc gcgtctggca tccgcgcggc cctgatcaag ttgaagtgtg ggcgttctgt
    39961 attactgaca aagccgcctc cgatgaagtt aaagccgctt ttgaaaacag cgccactcgt
    40021 gcttttggtc ctgctggttt tctcgagcag gatgactcgg agaactggtg tgaaatccag
    40081 aaattgctta aaggccaccg cgcccgcaac agcaaactgt gtctggaaat ggggcttggt
    40141 caggaaaagc gccgcgacga cggcattcct ggcattacta actatatctt ttcagaaact
    40201 gccgctcgtg gaatgtacca acgctgggct gatcttctga gtagcgaaag ctggcaggaa
    40261 gtgctcgata aaaccgccgc ttaccagcag gaggtgatga aatgagtgcg caagtttcac
    40321 tagagttaca tcaccgcatt agccagtttc tctttcacga agccagctta ctggacgact
    40381 ggaaatttcg tgactggctg gcgcagctcg acgaagagat tcgttacacc atgcgcacca
    40441 cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca gccaccgaca acctggattt
    40501 ttaatgacac caaagaccag ctggagcggc gaatcgcccg tctggaaacg ggcatggcct
    40561 gggcagaaga gccgccgtca cgcacccgtc acttaatcag caactgccag ataagcgaaa
    40621 ccgacatccc aaacgtattt gctgtgcggg taaattatct gctttatcgg gcacaaaaag
    40681 agcgcgatga aacattctat gttggaacgc gtttcgacaa agttcgccgt ctggaagatg
    40741 acaactggcg cttgctggaa cgggatatcg tcctggatca agcggtaatc acttcccata
    40801 acctgagtgt actgttctga tgaatcgaat ttatgcgtgt cccgttgcgg atgtgccgga
    40861 gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc ctgttcaacg ttggcggcga
    40921 gttttatgcc attaacgatc gttgcagcca tggtaatgcg tcaatgtcag aagggtatct
    40981 ggaagatgac gccacggtgg agtgcccgct acacgccgcc agtttttgcc tgaaaacggg
    41041 gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact tatccagtac acgttgaagg
    41101 tggtgacatt ttcatcgact taccggaggc gcagccatga gcgatctgca taacgagtcc
    41161 atttttatta ccggcggcgg atcgggatta gggctggcgc tggtcgagcg atttatcgaa
    41221 gaaggcgcgc aggttgccac gctggaactg tcggcggcaa aagtcgccag tctgcgtcag
    41281 cgatttggcg aacatattct ggcggtggaa ggtaacgtga cctgttatgc cgattatcaa
    41341 cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg attgttttat cggcaatgca
    41401 ggcatctggg atcacaatgc ctcactggtt aatactcccg cagagacgct cgaaaccggc
    41461 ttccacgagc tgtttaacgt caatgttctc ggttacctgc tgggcgcaaa agcctgcgct
    41521 ccggcgttaa tcgccagtga aggcagcatg attttcacac tgtcaaatgc cgcctggtat
    41581 cctggcggcg gtggcccgct gtacaccgcc agtaaacatg ccgcaaccgg acttattcgc
    41641 caactggctt atgaactggc accgaaagtg cgggtgaatg gcgtcggccc gtgtggtatg
    41701 gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg aaacctcgat aatgcagtct
    41761 ctgacgccgg agaaaattgc cgccatttta ccgctgcaat ttttcccgca accggcggat
    41821 tttacggggc cgtatgtgat gttggcatcg cggcgcaata atcgcgcatt aagcggtgtg
    41881 atgatcaacg ctgatgcggg tttagcgatt cgcggcattc gccacgtagc ggctgggctg
    41941 gatctttaag gaagcacgat gaaagaaaaa accatcatta ttgtcggtgg cgggcaagcg
    42001 gcggcaatgg ctgcggcctc gctacgccag caagggttca ccggtgagct gcatctgttt
    42061 tccgatgagc gacatcttcc ttatgaacga cctccactct cgaaatccat gttgctggaa
    42121 gattccccgc agttacagca ggtgttaccc gctaactggt ggcaggaaaa caatgttcat
    42181 ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca cacgagagtt agtgttaacc
    42241 aacggcgaaa gctggcactg ggatcagctt tttatagcaa ccggcgcggc agctcgaccg
    42301 ctgccgttgc ttgatgcact gggagaacgc tgctttaccc tacgccatgc cggtgatgcc
    42361 gccagactgc gagaagttct gcagcccgaa cggtcagtcg tgattgtcgg tgccggaact
    42421 attggtctgg aactggctgc cagcgccacg cagcgcagat gtaaggtgac agtgattgaa
    42481 ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg tgcaacgcta tcttttacag
    42541 cgccaccagc aggctggtgt gcgcattctg ctcaataatg ccattgaaca tgtggtcgat
    42601 ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc ttcaggctga tgtggtgatt
    42661 tacggtattg gtatcagcgc caacgagcaa ctggctcgcg aggccaacct tgatactgcc
    42721 aatggcattg tcattgatga ggcttgccgc acctgcgatc ccgcgatctt tgccggtggc
    42781 gatgtggcaa tcactcgtct tgataatggt gcactacacc gctgcgaaag ctgggaaaac
    42841 gccaataacc aggcgcaaat tgccgctgcc gcaatgttag ggctaccgct accgctactg
    42901 ccgccgccgt ggttctggag cgatcagtac agtgataact tacagtttat tggcgatatg
    42961 cgtggcgatg actggctttg tcgtggcaac ccggaaactc agaaggcgat ttggtttaat
    43021 ctgcaaaacg gcgtgcttat cggtacggtc acgctgaatc aggggcgtga gattcgccca
    43081 attcgcaaat ggatccagag cggcaaaacg tttgatgcga aactgctgat agatgagaac
    43141 atcgcgctta aatcactgta accaggataa ttagcgaata tctcaatgcc tggggcgtgg
    43201 cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc ttgcaaaggg attggttatg
    43261 aacacactac gttattttga ttttggagct gcccgccccg ttttgttatt aattgcccgt
    43321 atcgccgtgg tcttaatttt cattattttt ggttttccca aaatgatggg ctttgacggt
    43381 acggtccaat atatggcctc gttgggcgcg ccaatgccga tgctggcagc gattattgcg
    43441 gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg gctttttcac ccgtccgctg
    43501 gcggtgctgt ttattttcta cacgctgggt acggcggtga ttggtcacca ttactgggat
    43561 atgaccggcg atgcggttgg gccaaatatg attaatttct ggaagaatgt cagtatcgct
    43621 ggcgcattcc tgctgctggc aattaccggg ccgggggcaa tttctctcga tcggcgttag
    43681 gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt taataaatta cagccactct
    43741 acccgcaacg acatctctga agttgttgat tcccctggtg ctagcgcaat gagatcaccc
    43801 ccttccgggc gatgatgatc atccggcgca tggctcatcg gttccagaca gaagaaatca
    43861 aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga tgaaataaca cggtgcaggt
    43921 ggcgtcgttt ccatgatgat ggcataacct tcctgcggct gctcgatgcg ggcctgacca
    43981 ttccatccgg caaaaccatt gttcacccac tggcgcggca acggcgcggg ctggttaaaa
    44041 tccagttcct gcggtagctg ctcgcaaaac tcacccgcca gccactgctc ccgctccagc
    44101 cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg acaacggaaa ataaggatgc
    44161 cagccggtac caaatggcag cgtctctgcc ccttgattgg tgacagagag cgtcaccgtc
    44221 agcgtatccg ccgttaaatg aaacgcctga cttacccgat agtgatagac accgctgcga
    44281 tgttcataca ccagacacaa actatcatcg ctgtgcgaga cacattgcca ctcgcccagc
    44341 cagccatcgc cgtgtagata gtgcgcatcc cactcaacat tcggttgcag ttgatactca
    44401 cgcccctgcc agacaaaacg attgccgctc acccggtttg caaatggcac cagcggaaaa
    44461 catgaggcat cggttgccac accgcttttt ttaccaggac gtaagagcgg cgtcgtatcg
    44521 cgccagaagc cttcgattac gccgccctgg tcagaaacgt ccagctttag cgacccgtgg
    44581 gataaggtat agatggtcat ccgctaactc cttaatccgg gaagttaatc acaactttcc
    44641 cgcatttgcc gctcgccatc agcgcatagg catcacctgc ctgttccagc gagaagcgat
    44701 gggtaatggc gttacgcggc cacagtttcc agtccgtcag atcatgggcg catttttcca
    44761 tatggaacag actggtcacc caggagccga taatccgccg ttgatggtgc atcagatcgg
    44821 cgctgacctc gaactccact tttccggttt ctccgatgta aaccacccgt ccccagtcag
    44881 cggtggatcg cagtgccagc aagcgacctg cggcattacc agaacaatcg agcgcaacat
    44941 ccgcgccacc gtgggtgagt tcggcgataa tctgcggcag accttcggtg gttgctaaat
    45001 agccgtgatc catcacccct aactgttttg ccatcgccag acgttccggc agcatatcaa
    45061 cgccgatgat ccgttttgca ccgcgacctt tcgccagcat catcgccatc atgccgactg
    45121 gccccagacc gaccaccagc acgttatcac tgccggaaac ttcgccgcgc aaaattcctt
    45181 catacgctgt accaacgccg caactgataa acgcaccatc ttcgtagctc agcgcatccg
    45241 gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg ccgtcacgct
    45301 gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg cggcggcagt
    45361 tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc tctttaaaat
    45421 ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg ttgataaatc
    45481 cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga tagatatagt
    45541 ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg ttaatccccg
    45601 gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctggtaaa taagctgcca
    45661 gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct gccgcacccg
    45721 cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca gcaagatatt
    45781 cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa aggagttaat
    45841 accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa gcaatgtacc
    45901 gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg cggcgatgac
    45961 gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg ccgccagcaa
    46021 aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc gcactcgacg
    46081 aacgttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa aaaccgagcg
    46141 cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca tcatgatcag
    46201 cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct cgttttcgtc
    46261 aatcggcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcaggg cgctccacag
    46321 ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc cggcgagcgt
    46381 tcccatcagc gcgcctaaca gcaacaccag cagacacgcc accgccagcg gaacttcaaa
    46441 ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa cgctgacatc
    46501 aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa tggtggcggc
    46561 atcgcgcagc acgttcatct ggttattcaa tgagataaag cctggcgcgt tcagggagaa
    46621 gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat ggcgactgac
    46681 aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag cagacatctt
    46741 caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca gctcatccac
    46801 attgaccgga gcgtgaaact cctgcgagaa cgtgccgtgc tgtagtaaca ggatgcggtc
    46861 acagaccagc ggcaactcct ccacttcact ggagataaac accacgcttt ttccttcggc
    46921 agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga cgccgcgcgt
    46981 tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga tcaccacttt
    47041 ttgctgattg ccgccagaaa gcgtgccgat gggcgtttcg ctactggcgg ccttgaccgt
    47101 catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca gtacgccgtt
    47161 ggcgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca accagggaat
    47221 gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca gcatgtcgcc
    47281 gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt tcgccctgtt catactcctc
    47341 cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc cagcaatgcc
    47401 aagcacttcg ccgcgacgta acgtaaagct gatatcctcc agcttgggct tatggcgtaa
    47461 tgcacggact tccagcacgg cctgatccac aatttcctga ggggcaaccg gcgcaatatc
    47521 aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt tttcgagcat
    47581 cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa tgcggcgaat
    47641 ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca tctttttcac
    47701 cgcgatgatc accagttcaa cttccgcact cgcaagcgaa ctggtaggtt catcaagaat
    47761 gaccacgcgc ggctcgccct tcatcacccg cgcaatttcc accagttgct tttgcgccgg
    47821 gcttagcgtt gaaacaagtt gttcagggct aacgtcaacg cccagcgcct gtaagcaacg
    47881 ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcggg gccactgacc
    47941 gaggcagagg ttttccgcca ccgtcagtcc ctccaccaga cttaactcct gataaaccgc
    48001 acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt ccagtcgcgt
    48061 ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagca ttcgaatgag
    48121 agtcgatttg cccgcgccgt ttttacctaa cagcgcacga acttcgcctt tattgagcgt
    48181 gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg ccaccacttt
    48241 tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc agcgattagg
    48301 gcagaccatc aacgtgcgtt gccagccact gtttaccatc ttccgttttg gtatagagat
    48361 cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc ttcaatgttt
    48421 gcgcgaaaac agcattgccc attttcttac cggaaatatc cactaccgct ttcagcacct
    48481 gattgttttc cagctcctga gcaatttcgg ttgtcatatc cgaaccgaaa acagcaattt
    48541 ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca ccgcccgact
    48601 cccccataat ggcgttgaga tccggcgtgg agataatcag tttttcacca acggaaatcg
    48661 ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga acgcgggatt
    48721 ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct tcgcaattga
    48781 tgacagcaat tttcggctgg tcaattttat tggcaataaa ataatcggcg gcagcgttac
    48841 ccagtttttt accaaattcc agcggatcgc cgaccagata cgccgagaca tatttatcgc
    48901 cccccttttg attaatacag gtgttgtagc aaatcaccgg aatacccgct tcactggcgc
    48961 gacgaacggt acggctactg ccattttcag acactgccga taaaataatg gcatcgacat
    49021 tacgcgccac gagggtatca acaaaggtac tttctttcga aatatcaccc tgggcgttag
    49081 tttcaattaa ctgtacctgt actgaagaat cttttgccgc atcctgaacg ccctgacgca
    49141 ctccagcgta atatccctgg gtatcaaggt atattgcacc aatggtcatt tctttatccg
    49201 cagccctggc aaatagtgcg ctacctaata gcgtagccat taataataga ttacgggttg
    49261 ttctcatttt tttaggcata gtgcctcctg tagggttttt attaacaacg gcttattcta
    49321 attattttgt gatgagcggc agcgcgtgcc gctcaaatat tacagttaat agggtttagt
    49381 gaacgtagtt aaaaataaaa ggcatcacgg tggtgaataa tgccgctttc ggccaggccg
    49441 gattaatcgc cgtcaattca gcgcgagcag attcatattg cgcaacctcc ccgagtcccg
    49501 cggacgccag catcgccacc atcaggcatt tttctttatg ctgctgttgt aaatcgccat
    49561 acagcgacaa caggtcaggt tgtgaaacag caaagaaatc cgcctcaata ctggttttcg
    49621 ccatctcttg cgcccactgt ttcatttcac taaacagttg ctgtgcggtt tgctgttcac
    49681 ccagcagtcg cagcgccatt ccttgccaga agagataatc aaccggctga tcgttgtaat
    49741 aactgtggat gttaatggtg cgatcgccgg tcgccgccag acgtaaacaa cgcatcgctt
    49801 cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca gaaccagatg tcgttatcag
    49861 tttgccccgg taaacggcct tcgcttaaat tctccggata atgcagcgcg gcatgaagca
    49921 gttcgctggc ctgctgcggc tctctggcat caagatgctg ccaggcgcgt aataactggt
    49981 tgaggataaa ctgactggtg accttccctt ccccgccttc ccacgggtgg aatttacgcg
    50041 tggcgagaat gtccgccgct ttgtctgcct gacccgtgag atgccacaaa ttgagcagtt
    50101 ctgcggtcat gtcgtcgcgt ttcagcgcaa tttcctgatt attttccaga cgcgccagtc
    50161 gtttctccgg tgtggctcca cttaacttat caagcaaatc ccgttcgaaa agcagacgtg
    50221 catcctgcgg cgcaagctga taagcattat caagataacg cgcggccagc tcataatcgt
    50281 gttgcttatt ccacgcatgg atcgctaacc cgcgccagcc gtcggcaaac tccggcgaca
    50341 tctctacgca acgttgccat aaggcaatgg ctttgttgta gctacgtttg ttgtagtaga
    50401 agcaggccag taaatggcga gcaaaccagc actcttcaat actctccagc gccgccactt
    50461 cttccagcgt attcgggaaa cggacaaact gcgggaagac atcaatggct tttgcgacca
    50521 gttcgccacg ttcggctttc ggcagcaagc tggcttgcag gtaaagcggc agcgtgcgct
    50581 ggcagtccag tgcgttcagc atctctgccg ccagggtggg cattccccag ttaatcagtt
    50641 gcccggcggt catcagggcg ttaacgtcgc gtccctgaca cagaccgcgc cactgcgcga
    50701 gagctgattc gctacgacca tcgaaccagt tcagccacca cagagtggcg ttcagcggat
    50761 aatcgcgcag cagtttctcg cgctgcacac gcgcgttgtc ctgacgacca cttaacacca
    50821 gcagcagatt atgcaggcaa agcacttcct gattggttgg gcaggcgcga agactttgtt
    50881 ggcaaaaatc cagaccagcg tcgaagttac cattacgcgc cgccagtcgt gccaggccat
    50941 aatagccacc ggctttgctg ttgccgctcc agaccgcacg ccagaaatcc tcttcggctt
    51001 gttgatattg tccctgacgt tcgtaagcac tggcgcgaat caaactcgcc tgtccgcact
    51061 gcggattttt gttcagcgca tgtgcgcgtt tcagagcctg agtggcatac gccaccgctt
    51121 gcgggaaatc ggcgcggtta tattccagca tcgccagcgc caggttacag cggtaatcca
    51181 gcggatccag cgccacgccg cgcaggtagt aatcgaacgg tgaacggctg gcgtgatgat
    51241 attgctccag atgctgaccg ataaaccagg cttcatctgt actggtaatg tcttgtgctg
    51301 ccagtggcgc tttggcgacg tccggcaacg gcaactcttg cggctgatgt tcctgataac
    51361 tcagtacaat attgccgtcg gcatcggaga gctcaatggt cagcctttca ggattgatac
    51421 cgtgcaacac gccctggatg gcggtcgcag gcatcagtgc cacggcatca tcaagtaacg
    51481 cgttgcattt gccgatttcg cggatcgcca ggcggtatcc gttcaacgga gagatggcat
    51541 acagccccca ctcaatcccc cgcttactac gctggagttt tatcaccgca tcgcgggagg
    51601 cattttgcac catgcccaaa gaatgataag gcaggaaata ctgctcaaaa cgcttctctt
    51661 cgtaagcatc aagccaggta aaatcgggct ggttatcggc aaaaataccg gtcatcagtt
    51721 cgatatacgg accgttattg tcggtcagac tcttatccca cgcctggcca aattcactgt
    51781 gtccccaact ccactgtttt ttacctggcg caatatggtg gttggcaacg tgcagcaaac
    51841 cgccatcttc atcgtgacac cacgcgccga caaaatcgta ctgcgatttt tcggccatat
    51901 atgaggttgg aacaggcaca tttttatagc gagaaatgtc cactccggcg gagtagtcca
    51961 ctttgtagta agtgccggtg gcgatgggga aagcggagac ggcccgtttg ccgtgatcaa
    52021 acaccgcagt cacatccggt gggaaaacgc tctgatgccc ttccccccct ttcactgccg
    52081 ggttggccca ccacaagaaa tgacgcggcg tggcgttacc gttatagacg cggctggcga
    52141 tttccagcgc cgcccggtca gggcgcaggg tgaaacctgt catcacctgt aaaccatgca
    52201 tcggctccgt ttcgcctacc cacaccgtct gtgcaccgtc ttcatgggct tcgagggtga
    52261 aatcaacggg cataaaggtg gtcgggcgat gatgttgcgg ccagttaaac tcaatcccac
    52321 cggaaatcca cggccccagc agccccacca gcgcaggttt aatgacttca ttgtgataaa
    52381 caaaatcgcg ctgtttcact ttatcccacg cgcgatgcac ccgaccgccc agttccggca
    52441 ggatcatcac tttgatgtag tcgttttcca gccacaccgc ctgccaggat ttcagggttt
    52501 tctgctcgct cagcgtatcg gtcacgccgt agggataaac cgcgccggac gatccctgat
    52561 aaacgcgatt ttcgaggaac atgggatgta tatcctgcgg cccggtttca taggtcggga
    52621 tctcaacgcg ctcttgccac acttttactg gagtcatggt gccctcaata ttaacaagac
    52681 atactgaatt aaaagatttg tggcagtgta ttgaacaatc tggcaatgtt ttcgcggaat
    52741 aatcacgcaa ttaactaaac aaggtttagt gaagatgaga gcctgcatta acaatcaaca
    52801 gattcgccac cataacaaat gcgtgattct ggaactgctg taccggcaaa agcgcgccaa
    52861 taaatcaacg ctggcccggc tggcgcaaat ttcgattccg gcagtcagta atattttgca
    52921 ggaactggaa agcgaaaaac gggtggtgaa tattgacgat gaaagccaga cgcgcgggca
    52981 tagtagcggt acatggctga ttgcgccgga aggtgactgg acgctgtgcc tgaacgtgac
    53041 gcccaccagt attgagtgtc aggttgctaa tgcttgttta agtccgaaag gtgaatttga
    53101 gtatttacag attgatgcac cgacgccgca ggcgctgctg tccgaaatcg aaaaatgctg
    53161 gcatcgccac cgtaaattgt ggccggaccg caccatcaat ctggcgctgg caatccacgg
    53221 tcaggttgat cctgtgaccg gcgtgtcgca aaccatgccg caagcgccgt ggacaacgcc
    53281 ggtggaggtg aaatatctgc tggaagagaa gctcggcatt cgggtgatgg tcgataatga
    53341 ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat tcgcaggaac gggatttctg
    53401 cgtgatcaac gttgattacg gcattggctc gtcgttcgtg attaacgagc aaatttatcg
    53461 cggcagtttg tatggtagcg gacagattgg tcacaccatc gttaatccgg atggcgtcgt
    53521 ctgcgactgt ggacgttatg gctgcctgga aaccgtcgcc tcgttaagcg cattaaaaaa
    53581 acaggcgcgg gtatggctaa aatcacaacc ggttagtact caacttgatc ctgaaaaact
    53641 gactacagcg cagttaatcg ctgcctggca aagtggagaa ccgtggatca ccagctgggt
    53701 tgaccgcagt gccaatgcca ttggtttgag tctgtataac ttcctcaaca tcctcaatat
    53761 taatcagatt tggttgtacg gtcgcagttg tgcctttggt gagaactggc ttaatactat
    53821 tattcgccag acaggattta acccgttcga ccgcgacgaa ggaccgagcg tgaaagcgac
    53881 gcaaattggc ttcgggcaat taagccgcgc acaacaggtg ctgggaattg gctatttgta
    53941 tgttgaggcg cagttacggc agatttgatg gcgcaataac gtagaaaggc ttcccgaagg
    54001 aagccttttt tcaacagatt aaccgtttcg cttatgcgta aaccggataa cgtgcgcaga
    54061 tgtcgagaac tttacctttg atgcgctcga taacagcttc atcattgatg ctgtccagca
    54121 cgtcacacat ccagccagcc agttctttcg cttctgcttc tttgaagccg cgacgcgtaa
    54181 ttgccggagt acccacgcga ataccggagg tcacaaacgg gctcttcgga tcgttcggta
    54241 cgctgttttt gttgacggtg atgttagcac ggcccagagc ggcgtctgct tctttaccgg
    54301 tcaggttttt atcaaccaga tcaaccagga acaggtggtt atcagtgccg ccggaaacca
    54361 ctttgtagcc gcgctcgagg aacacttcta ccatcgcttt agcgtttttc gcgacctgct
    54421 gctggtaagt tttgaactca ggctccatcg cttctttcag agcaaccgct ttaccggcga
    54481 ttacgtgcat caacggaccg ccctgaccac cagggaaaac ggcagagttc agttttttgt
    54541 acagctcttc gctaccacct ttcgccagga tcaggccgcc gcgcggaccc gccagggttt
    54601 tgtgagtggt ggtagtaaca acgtgagcat gaggaaccgg gttcgggtag acgccagcag
    54661 caaccaggcc cgcaacgtgc gccatatcaa cgaacaggta agcaccgatg ctgtcagcga
    54721 tttcacgcat tttcgcccag tccaccacgc cggaatatgc agagaaacca ccgataatca
    54781 ttttcggctt gtgttctttg gcttgttttt ccagatcggc gtagtcgata tgaccggtag
    54841 catcgatacc gtaaggaacg atgttgtaca gtttaccgga gaagttaacc ggagaaccgt
    54901 gagtcaggtg accgccatgc gccaggttca tacccagaac ggtatcacct ggttccagca
    54961 gcgcggtgta gaccgcaaag ttagcctggg agccggagtg cggctggacg ttagcgtagt
    55021 cagcgccgaa cagttctttc gcacgatcga tcgccagttg ttcaacgata tcaacatact
    55081 cgcaaccgcc gtagtagcgt ttgcccggat aaccttcagc atatttgttg gtcagctgag
    55141 aaccctgcgc ctgcattacg cgcgggctgg tgtagttttc ggaggcgatc agttcgatgt
    55201 gctcttcctg acgtactttt tcctgctcca tagcctgcca cagttcggca tcataatcgg
    55261 caatgttcat ttcacgcttt aacatccgca tctcctgact cagctaacaa taaaattttt
    55321 ggcctttata ggcggtcctg ttggacaacg gcgaacagta taaccgaatc attgtgcgat
    55381 aacaggtctt gacaaaggaa tttacgcaaa cgattacctt caggctacgc aaggctttgg
    55441 agaataaaga gcttgcaacc ggaaacggat ttcttttcag gtttgtgatg caaatttttc
    55501 acttcatcac attctttctg aaaaacacca aagaaccatt tacattgcag ggctattttt
    55561 tataagatgc atttgagata catcaattaa gatgcaaaaa aaggaagacc atatgcttga
    55621 cgctcaaacc atcgctacag taaaagccac catcccttta ctggtggaaa cggggccaaa
    55681 gttaaccgcc catttctacg accgtatgtt tactcataac ccagaactca aagaaatttt
    55741 taacatgagt aaccagcgta atggcgatca acgtgaagcc ctgtttaacg ctattgccgc
    55801 ctacgccagt aatattgaaa acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca
    55861 gaagcacacc agcttccaga tcaaaccgga acagtacaac atcgtcggtg aacacctgtt
    55921 ggcaacgctg gacgaaatgt tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc
    55981 ctatggtgta ctggctaatg tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc
    56041 cagcaaagcc ggtggttggg aaggtactcg cgatttccgc attgtggcta aaacaccgcg
    56101 cagcgcgctt atcaccagct tcgaactgga gccggtcgac ggtggcgcag tggcagaata
    56161 ccgtccgggg caatatctcg gcgtctggct gaagccggaa ggtttcccgc atcaggaaat
    56221 tcgtcagtac tctttgactc gcaaaccgga tggcaaaggc tatcgtattg cggtgaaacg
    56281 cgaagagggt gggcaggtat ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt
    56341 gaaactggtc gctccggcag gtgatttctt tatggctgtc gcagatgaca caccagtgac
    56401 gttaatctct gccggtgttg gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa
    56461 agcaggccac acagcacaag tgaactggtt ccatgcggca gaaaatggcg atgttcacgc
    56521 ctttgctgat gaagttaagg aactggggca gtcactgccg cgctttaccg cgcacacctg
    56581 gtatcgtcag ccgagcgaag ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat
    56641 ggatttgagc aaactggaag gtgcgttcag cgatccgaca atgcagttct atctctgcgg
    56701 cccggttggc ttcatgcagt ttaccgcgaa acagttagtg gatctgggcg tgaagcagga
    56761 aaacattcat tacgaatgct ttggcccgca taaggtgctg taatttgatg ttgccggatg
    56821 gaaacatccg gcaacccttg acgcggttta aattgccgcg tcgtcctctt caccggtacg
    56881 gatgcgaatg acccgtgcca cgtcgaagac gaagatttta ccgtcaccga ttttgccggt
    56941 ttgcgccgtg cgaataatgg tatcaacaca ggtatcgaca atgtcgtccg gtacgacaat
    57001 ctcaattttc actttcggca gaaaatccac catatactcc gcgccgcggt acagctcggt
    57061 atggcctttc tggcggccaa aacctttcac ctcagtgacc gtcatgccgg taatgccgac
    57121 ttcagccagt gcttcgcgaa catcgtccag cttgaagggt ttaataatcg catcaatctt
    57181 tttcatgcta ttccttgaaa aggtcgcctg tcttttgatc tgctaaacgt aacacataac
    57241 gccaattcat tccttgaaat cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa
    57301 ttctgtccgg ttgcgccccg ccattctcgc cgcgtgtgtg acgttgcctt tggtgatttg
    57361 cagcagctta cgcaaatagt tgagttcaaa ctgattacgt gcctcaacaa aggttggcag
    57421 cgccgtattt tcaccctcca gcgcctgctc tactaatgca tcgctaataa ccggagatga
    57481 ggtcagcgcc acgcactgtt caatcacgtt gaccaactgg cgcacattac ccggccagct
    57541 agcggtcatc aggcgtttca tcgcatcagt ggagaacgcg cggacaaacg gtttatgtcg
    57601 ctctgccgcc tggcgcaaca ggtgatttgc cagaagcgga atgtcttctg tgcgctctgc
    57661 cagagccgga attttcaggc tgacaacgtt gaggcggtaa tagaggtctt cacggaattc
    57721 cccgcgcgcc atcgcttttg gcaggtcgcg gtgagtggcg gaaataatcc gcacgtcgat
    57781 atcaatatcg cggttactgc ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag
    57841 tttgacctgc aacggtgcgg gcatatcgcc aatctcatcg agaaatagcg taccgccttc
    57901 cgccgcctgg aataaacctt cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg
    57961 accaaacagc tccgactcca gcaattgctc gggcaatgcg ccacagttaa tagcaataaa
    58021 tggtttgcta ttgcgcgggc tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt
    58081 gccgctctgg ccgttaatca aaacgctaac gtctgattgc gccaccagcc gcgcctgttc
    58141 cagcaaacgc agcatcagcg ggctgcgggt gacaatcgcc tcgcgccagc gttcatcggt
    58201 ggctggcgcg gattgttcca gcgcatcgtc aattgcctga tatagcgcat ctttatcgac
    58261 aggcttggtg aggaaactga acacgccctg ctgtgttgca gcaacggcat cgggaataga
    58321 accatgcgcg gtaagaataa ttactggcat tcccggctgc actttctgaa tttcagcaaa
    58381 cagctgcata ccgtccattt catccatccg caggtcgctg atgactaaat ctactttttc
    58441 gcgattcagt acccgtaatc cttcagcgcc actttccgcc gtgaccacgc tgtagccttc
    58501 gctggtcagg cgcaggccaa gcagtttcag caatcccgga tcgtcatcga ccaataataa
    58561 atgcgcaggt ttatggctca tcaggagtga cctcatgggt ggatggcgcg ggcttttcac
    58621 cctcgtgcgg cgtatccggc gagaaatttc cagccggttt gcgggtcgag agctggcgtt
    58681 caatatcagt caggttttcc agcttgcggg tggtgagttc cagttgctgc tgtagaacgt
    58741 gatgttgctg gcgcaatgta tccagctcgc tgtcgctggc ctgctggagc ttgccgtagc
    58801 gttggcgctc ttccgccagt tgcagttgca gcgcctgacc gtcgcgccag agttgataca
    58861 gagggcgaac ctgtgccggg atctcggtac ttagcgcatc aatgcgcgcg accagctggc
    58921 ggcgctcata cggcgtaatt ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt
    58981 gccagctgcc gtcgtcatat tggcgagctt gctgacgtga ctgcgcaggc attaaacgat
    59041 cagcacaatc catcgcccgc agccagtaaa gcggattggt ttcggttgat ttaccttgca
    59101 gcgcccagat gtcgctacat tcagtagaaa gatagtcagc cagttgataa accggaattt
    59161 tttcttctgc tggcgtatca atggctggct tattgtgact ctgcacgcaa cccagcaatg
    59221 ccagacatgg cagccctgcc agccacagcc gtcggggcaa taatcgttga aaaatgtgtc
    59281 gcatattcac cagacttaat gcctatccca gtgggcgtaa ttattgcagg cagtttggac
    59341 atggacagcg cggagaaacc ggagcgtaca cgtcgtacgt gaggatttcg agcactgccc
    59401 ggggccgaac tggcaaataa aatagcctga tgggataggc tgttagcatt atttcgtgtt
    59461 tttcgacgac ggcaattcaa tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata
    59521 cagttcccct tgcatacggc aaatacaatc cctggcaatg cttaatccca gaccgctgcc
    59581 cttcaccgcc ccttttcgct ggtggcttcc ttgaaaaaag ggttcgaaga tcatggcgcg
    59641 ttcctcttgc ggaatgggtg tgcctgtatt gatgacatca atataaaccc gtgcaccatg
    59701 taagctgctg cgaaggcaaa tgttaccgga ttcagccccg tagtgcaccg cattggagta
    59761 aagattatcc agtacgctca tcagcagcat tggctccgcc aggcaagctg ttgctttgag
    59821 atcgacgtcg gtatgcatca ttttagcccg tgcgggcagg ctatgagcag aaaccactgt
    59881 ctccaccagc ggtgctaatt caacattctc cagttccacc gcactgtccg cctgtttacg
    59941 gttgtaatca agcagttgtt cgatcagttt ttgcaaattg cggctgctgc tatcaagaat
    60001 gctcaccacc tctttttgct ctggcgtaag cggcccgaca acctggtcag ccagtaattc
    60061 agtgccctcg cgcatactcg ccagtggcgt ttttaattca tgagataaat gtcttaaaaa
    60121 ttgatggcgt tgggattcca gccatgacag gcgctcactt aaccaaagaa tacgttgccc
    60181 aaccgagcgt aactcgctcg gtccactgaa cgaaacgcta ttgcccagag aacgcccttc
    60241 ccccagacgg ttgatcatgc gctcgatatt tttcaccggc ccgataatca tccgcgtgaa
    60301 aagtagcacc atcaccagac tcaccagaaa tagcaccagc gattgccaac caaaatattg
    60361 cccacgttcg gcgatttcac gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt
    60421 ggcctgcacc atttcggtat tggcactggc aaaggcttca agacgcgcgg cggcggcagc
    60481 atcgggacca ctgttgttac actgaagttg ggccagattg ttcaaatcct gacgcaatgc
    60541 ctggtaaagt ttatcgtctg gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg
    60601 cttgcgctgg ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc aatactgacg
    60661 gtagctacgc tccatctcca gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat
    60721 aagcgtagtg cggttaacca gcgccgcctg atcgctaagc gcattcaggc tttgccaggc
    60781 ttgccatgcc agcaccaaca ggggtagtag aattagcaaa aatgccagca ttaccagttg
    60841 tcgtaatgag cggggaaaaa cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa
    60901 tagcctgatg ctaaccgagg ggaagttcag atacaacaaa gccgggaatt acccggcttt
    60961 gttatggaat aaggcggtgc ctaactcgac gtttcgcccg atggttgata tagctacgct
    61021 gatatcagaa gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt atgtagcaca
    61081 tcccgaaggg gctgacataa gtcggtgaat gagccactgc ttactattat gcagtaactg
    61141 tgccaataaa gaaaatagtt tggtaacgta ctgattatac gttgctttga gggatttatg
    61201 gcttcttcgc tatttgaatg taaatcagcc actgtgtcgc taaaaagaga caacttaaga
    61261 taaacttatt agattacaat aaaatcaatc atttaagtgt cgccagaaag cgacacggta
    61321 aaccacccat tgtcgtgatt tacagacaca aaaaagctcc cggagttggg agcttatgat
    61381 agtggttggt gcttaatgcc ggatgcggcg taaatgccgc atccgggctg caaaaccaat
    61441 gaactgatga cttaccccaa ctgcttacgc gcattgcgga aaatgcgcat ccatgggcca
    61501 tcctcgcccc agttttccgg atgccaggag ttgctgacag tacggaaaac acgttccggg
    61561 tgcggcatca taatggtgac tcgaccactt tcagtcgtaa ccgacgtaat accgttcgga
    61621 gaaccgttcg ggttagccgg gtaggtttca gtgactttgc cgaagttatc gacatagcgc
    61681 agtgccacca gccctttgct ttccagtgcc gccagatgcg ccgcatcacg cacttccacg
    61741 cgcccttcac cgtgagagac agcaatcggc atttgcgagc ccaccatccc ctgcaacagc
    61801 agcgacgggc tttgggtcac ttcaaccagg ctgaaacgcg cttcaaagcg atcggaggta
    61861 ttgcgcacaa aacgtggcca caactcacta cctgggatca gttcacgcag attagacatc
    61921 atctggcaac cgttacatac ccccagcgcc agcgtttgcg gacggtggaa gaaggttgca
    61981 aactcatcgc gtacacggtc attgaacagg attgacttcg cccaaccttc accggcaccc
    62041 agcacatcac cgtaggagaa accaccgcac gcgaccaggg cgtggaaatc ttccaggccc
    62101 gtgcgtccag ccagcaggtc actcatatgc acgtcgatag catcaaagcc tgcacggtgg
    62161 aaagctgccg ccatttcaac atgcgagttc acgccctgct cacgcagtac ggcaactttc
    62221 gggcgcgcgc cagtggcaat aaacggtgct gccacatctt cgttgatatc gaacgacagt
    62281 tttacattca ggcccggatc ggcgtcgttt gatttcgcct ggtgctcctg gtcagcacac
    62341 tccgggttgt cacgcaggcg ctgcatctgc caggtagttt ctgcccacca gacacgcaac
    62401 gtagtgcggc tttcgctgaa tacagtctgc ccgttggcgg taatcacaaa acggtcaccg
    62461 gaaaccgcct gcccaacata atggacgcaa tcagcaagcc catgctgtgc cagtacggac
    62521 tcgaccgctt cacggtcagc ggcacgaacc tgaatcaccg cacccagttc ttcgttaaac
    62581 aacgccgcca ggcgatcgtc acccagagag ccgatatccg catcaatgcc acaatgacca
    62641 gcaaaggcca tttccgccag tgtgaccaac agaccgccat cagagcggtc gtgatacgcc
    62701 agcagcttac gctgtgcaac cagcgcctga atcgcgtcat agaagccttt cagttgcgcg
    62761 acatcgcgta catctgccgg tttgtcgcca agctgacgat aaacctgcgc cagcgccgtt
    62821 gcgcccagcg cgttattgcc tttgcccaaa tcaatcagca gcagtgcgtt atcttcggta
    62881 gaaagctgcg gcgtgatggt gtgacgtaca tcttccacgc gggcaaatgc agaaatcacc
    62941 agcgacagcg gcgacgtcat ttcgcgctct tcgttacctt cctgccagcg ggttttcatc
    63001 gacatagagt ctttacccac cgggatcgtc aggcccagcg ccggacaaag ctcttcgccc
    63061 acggctttaa cggcttcata caggcccgca tcttcaccag ggtggcctgc cgccgccatc
    63121 cagttggcgg aaagtttaat gcgtttgata tcgccaatct gtgttgcggc aatattggtt
    63181 aacgcttcac cgaccgccag acgggcagag gcggcgaaat ccagcagcgc aaccggcgca
    63241 cgctcgccaa tcgccatcgc ttcaccgtag tagctgtcga ggctggcggt agtgaccgcg
    63301 cagttagcga ccggcacctg ccacggaccc accatctgat cgcgcgctac catgccagtt
    63361 acgctgcggt caccgatggt caccaggaaa gttttctccg ccacggtcgg cagatgcagt
    63421 acgcgtttca ccgcgtctgc aatggtgatc ccttcacgga ccagcgcatc gcccttcgct
    63481 ttcagcgttt gtacatcgcg ggtcatcttc ggcgttttac caagcaggac gtccagcggc
    63541 agatcgatcg gctgattatc aaaatgacga tcgtgcagag aaagatgcag ttcttcggtc
    63601 gcttcaccaa tcaccgcgta gggtgcgcgc tcacgcttac acagttcgtc aaacagcggt
    63661 aattgatcgg cagcaaccgc cagcacgtag cgttcctggg attcgttaca ccagatttcc
    63721 agcgggctca tgcccggttc gtcgcttaga atatcgcgca gttcaaattt accgccgcgt
    63781 ccgccgtcgc tcaccagctc cggcatcgcg ttagaaagac cgcccgcacc gacgtcgtgg
    63841 ataaacagaa ttgggttagc atcaccaagc tgccagcaac ggtcgatcac ttcctggcaa
    63901 cgacgctcca tctccgggtt gtcgcgctgt acggaagcaa agtcgaggtc ggcatcagac
    63961 tgacctgacg ccatggaaga cgccgctccg ccgccaagac cgatgttcat tgccgggccg
    64021 ccgagaacga ccagcttcgc accgacgttg atctcgcctt tttgtacgtg atcggcgcga
    64081 atgttgccga tcccgcccgc cagcatgatc ggtttgtgat aaccgcgcag ctcttcgccg
    64141 ttgtggctgt tcactttttc ttcataagta cggaagtagc cattcagtgc cggacgacca
    64201 aattcgttgt taaacgccgc gccgcccagc gggccttcgg tcatgatgtc cagcgcggtg
    64261 acaatgcgct caggcttacc gaaatcttct tcccacggct gttcgaagcc aggaattcgc
    64321 aggttggaaa cggagaaacc aaccaaaccc gctttcggct tcgcgccgag gccggtggca
    64381 ccttcatcgc ggatttcacc gccggaaccg gtcgccgcgc ccggccacgg agaaatcgcc
    64441 gtcgggtggt tgtgagtttc gactttcatc agaatatgcg ccggttcctg atggaaatcg
    64501 tagcggcccg tttcgtggtc agcaaagtag cggcccactt cagaaccttc cattacggcg
    64561 gcgttatctt tataagcaga gagaacgtaa tctggcgtgg tttcgaaggt gtttttgatc
    64621 atcttaaaca gcgatttcgg ctgttgctca ccgtcgataa cccaatcggc gttaaaaatc
    64681 ttgtggcggc agtgctcgga gttcgcctgg gcgaacatat acagctcgat gtcgttcggg
    64741 ttacgaccaa gctttgtgaa agcatcctgc agatagtcaa tttcatcttc cgccagtgcc
    64801 aggccaagac gcaggttagc gtcgattagc gcctgacggc cctgccccag caaatcaacg
    64861 ctggtgaccg gagtcggttg atggtgtgca aacagctgct ctgcatcatc taaagcaaaa
    64921 aagaccgttt ccatcatgcg gtcgtgcagt tcagcggtaa cctgctgcca ttgttcattg
    64981 gtcagcgtac cggcttctat atagtaagca acgccgcgct caaggcggtt tacctgttgt
    65041 agcccgcagt tatgggcaat atcggtcgct ttcgaagacc agggagagat ggtgccagga
    65101 cgcggggtca ccagcaggag tttgccttgc ggggcgtggc tggcgagtgc cgggccatat
    65161 ttcagcaggc gttcaagttg tgcgtgctca tcatcgttta acggcgcatt gaggtcagca
    65221 aaatggacat actcggcgta aatattgtga accgggagcc tggcagcctg aaaacgtgcc
    65281 agcagtttgt tgattcggaa tgccgacagt gcaggcgaac cacgcagaat ttccatcata
    65341 agtctctcgt cttctaagct ttcggtgtac ccaagggggg gaaacgggcg tcattataaa
    65401 gaatctgatg cgctgacgaa accgtttgcg tggaaataaa atcaccatcg tgaattagca
    65461 acgcgtgccg ccaatggctg taataagttg ccatctggcg caggtttacg caaaatgccg
    65521 ctcatttaag agtaaacctt tcactattat tacgtttttt caagctggga cgcgcacgac
    65581 acagagaatt aactaattga aaaaattaaa gattaattat ctgttcatcg gcattctggc
    65641 actgctgctc gcggtcgctc tctggccatc cattccctgg tttggtaaag ccgacaaccg
    65701 tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc accattcata ctcccctgac
    65761 ttataacgaa atcaacggga aaccttttgg cctggattac gaactggcga aacagtttgc
    65821 cgattacctc ggcgtaaaac tgaaagtgac cgtgcggcag aatatcagcc agctgtttga
    65881 cgaccttgat aatggtaacg ccgacctgct ggcggcagga ctggtctata acagtgagcg
    65941 ggtaaaaaat tatcagcctg gccctaccta ttattccgtg tcacaacaac tggtttataa
    66001 agtgggtcag tatcgcccac gtacgctggg caacctgacg gcggagcaac tcaccgttgc
    66061 accgggtcat gtggtggtta acgatctcca gaccctgaaa gaaacaaaat tcccggaatt
    66121 aagctggaag gtagacgaca aaaaaggctc tgcggaatta atggaagatg tcatcgaagg
    66181 aaaactcgat tacaccattg ctgattctgt cgccatcagc ctgtttcagc gcgttcaccc
    66241 ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg gtgacttggt ttagcccgtt
    66301 agatggcgat aatacccttt ccgccgccct gctcgacttc ttcaacgaaa tgaatgaaga
    66361 cggtacgctg gcacgcattg aagagaaata cctggggcat ggcgatgatt ttgattacgt
    66421 cgatacgcgc acatttttac gcgccgtcga tgcggtactg ccgcagttaa agcccctgtt
    66481 tgagaaatac gccgaagaaa ttgactggcg tttgctggcc gctattgctt atcaggaatc
    66541 gcactgggat gcacaggcca cttcaccgac gggtgtgcgc ggcatgatga tgttaaccaa
    66601 aaataccgcg caaagcctcg gcattacgga tcgtaccgat gccgaacaga gcatcagcgg
    66661 tggcgtgcgt tatttgcagg atatgatgag taaagtgccg gaaagtgtgc cggagaacga
    66721 gcggatctgg tttgccctcg ctgcgtacaa tatgggctat gcgcatatgc tggatgcccg
    66781 cgccctgacg gcaaaaacca aagggaatcc tgacagttgg gctgacgtaa aacagcgtct
    66841 gcctttactt agccagaaac cctattacag caagctgact tacggctacg ctcgtggaca
    66901 tgaagcctac gcttatgtcg aaaatattcg taagtatcag attagcctgg tgggttatct
    66961 gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa ctggcgcagg attatccggc
    67021 ggtatcgcct acggagttgg gcaaagagaa atttcctttt ctctcgtttc tttcccagtc
    67081 gtcatcaaac tatttgaccc attctccctc tctgctgttt tccaggaaag ggagtgaaga
    67141 gaaacaaaat taatccgtcg aggattgcgc ttttttctgc gctttaattt cctggcggcg
    67201 catgcgaaag aagtcactga gcaacgccgc gcactcatcc gccagtattc cttccgtaat
    67261 ttccactcgg tgattcatac ccggatgatg cagcacatcc attaaagatc ccgcagcgcc
    67321 agttttcgcg tcacgcgcac caaagaccac gcgaccaatg cgactgtgga tcatcgctcc
    67381 ggcacacatt acacatggtt caagcgtgac atacaacgtg gcgtcgatca gacgataatt
    67441 ttgcatcacc agaccaccct gccgcagggc catgatttct gcatgtgcgg tgggatcatg
    67501 gcgaccaatc gggcggttcc agccttcgcc gattacccga ttgttatgca ctaataccgc
    67561 gccgaccggc acttcccgct catcccaggc acgtttcgcc agcgtcagcg cgtgacgcat
    67621 ccagtattcg tggctaaatt cgacttcaga caaaaagaaa actccggtta taaaagcgcg
    67681 gcgcattata cacggacgct atgctttact ctattccagt tgctggagtt caccgcgcgg
    67741 ggttactcgc caacgatgct gacagaaata aagcagcgga ttgtcctgat tactgtcgct
    67801 atagccactg tacagccgca gcggagtgcc gattttgcgc tccagttgtg cgaccttttc
    67861 atgtcccaga caacgcatcg tcaataccca accaccatag ccacgctgaa tttggctggc
    67921 gataagatta acccgcggca gccagggcgt atcgaaataa accgcttcaa ccagcggctg
    67981 cggagagccg gtaatcaacc agatatcagc atcggaactt aacaggtagg tggttaatcg
    68041 ctcctgaacc agcggaaagg cggtaacatt gtcgcgaaac cagcgcacga aatcggcctg
    68101 caacgtctgt aaacgtgctt cgctgtgacc aaaagtgcac ccccacagaa gcagactcat
    68161 cggccagcgt gccgcacgac cttttatcaa taacgcaatg gctataatcg gtaacaacgg
    68221 caggacaagt aacgcattca gcggttggcg acgcagtaaa tagcgcagaa aactgccgaa
    68281 catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac accacacgac gctcgtgagt
    68341 tgccaaacca tactcctctg gactgaaaca tcctgattaa ttttcgctac tacatagcct
    68401 aacagataga tcatcacttt tccggcaaca atcgaccgca tcatggctaa ctggaatttt
    68461 taattcatgg caattagcgg caatggaata taaaattcac tcgcgtgtct ctcatattgc
    68521 ctgttgtcgc cactatttca tcgcgaggaa agatatgaac ggcttacttc gtatccgtca
    68581 gcgttaccag gggcttgccc agagtgataa aaaactggcg gattatctgc tgctacaacc
    68641 tgatacggcg cgccatttaa gctctcagca actggccaac gaagccggag tcagtcagtc
    68701 cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt tttccggcgc ttaagttggc
    68761 gttgagtgaa gcgctggcaa gccagccgga atcgccctcc gtgcccattc ataaccaaat
    68821 ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg attaaagaaa atactgccgc
    68881 tatgtacgca acgctaaacg ttaatagtga agagaaactg catgaatgcg taacaatgtt
    68941 gcgctctgcg cggcggataa ttctgaccgg tattggcgct tcgggtctgg tggcgcaaaa
    69001 ctttgcctgg aagctgatga agattggctt caatgctgcc gcagtgcgcg atatgcatgc
    69061 gctactcgca acagtacagg cgtcgtcccc tgacgatctg ttattagcca tttcctacac
    69121 cggtgtacga cgcgagttaa acctggcggc agatgagatg ctgcgagtgg gcggaaaagt
    69181 gctggcgatt accggcttta ctccgaatgc cctgcaacag cgtgcttctc attgcctgta
    69241 taccattgcc gaagaacaag cgacaaacag cgcttcaatc tctgcttgtc acgctcaggg
    69301 aatgttaacg gatttgctgt tcattgcgct gattcagcag gatctggaac tggcaccaga
    69361 acgtattcgt catagtgaag cgctggtgaa aaaactggtc tgagtaaaga atgcgcgtat
    69421 aatgcccgcc cggtttgtgt tgttttgaga gtttccttat ggcgttgtta atcactaaaa
    69481 aatgcatcaa ttgtgatatg tgtgaacccg aatgcccgaa tgaggcgatt tcaatgggag
    69541 atcatatcta cgagattaac agcgataagt gtaccgaatg cgtagggcac tacgagacac
    69601 caacctgcca gaaggtgtgc ccgatcccca atactattgt gaaagatccg gcgcatgtcg
    69661 agacagaaga acagttgtgg gataaatttg tgctgatgca ccacgcggat aaaatttaat
    69721 attctac
//
LOCUS       DAZFKM010000008        66290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFKM010000008 DAZFKM010000000
VERSION     DAZFKM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..105
                     /locus_tag="ACHKAY_000585"
     CDS             <1..105
                     /locus_tag="ACHKAY_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5699315.1"
                     /translation="TTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            116..952
                     /gene="eutJ"
                     /locus_tag="ACHKAY_000586"
     CDS             116..952
                     /gene="eutJ"
                     /locus_tag="ACHKAY_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO5699316.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGGDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            942..2129
                     /gene="eutG"
                     /locus_tag="ACHKAY_000587"
     CDS             942..2129
                     /gene="eutG"
                     /locus_tag="ACHKAY_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO5699317.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2246..3472
                     /gene="eutH"
                     /locus_tag="ACHKAY_000588"
     CDS             2246..3472
                     /gene="eutH"
                     /locus_tag="ACHKAY_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO5699318.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3469..4872
                     /gene="eutA"
                     /locus_tag="ACHKAY_000589"
     CDS             3469..4872
                     /gene="eutA"
                     /locus_tag="ACHKAY_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO5699319.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4884..6245
                     /gene="eutB"
                     /locus_tag="ACHKAY_000590"
     CDS             4884..6245
                     /gene="eutB"
                     /locus_tag="ACHKAY_000590"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO5699320.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6266..7153
                     /gene="eutC"
                     /locus_tag="ACHKAY_000591"
     CDS             6266..7153
                     /gene="eutC"
                     /locus_tag="ACHKAY_000591"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO5699321.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7163..7822
                     /gene="eutL"
                     /locus_tag="ACHKAY_000592"
     CDS             7163..7822
                     /gene="eutL"
                     /locus_tag="ACHKAY_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO5699322.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7835..8335
                     /gene="eutK"
                     /locus_tag="ACHKAY_000593"
     CDS             7835..8335
                     /gene="eutK"
                     /locus_tag="ACHKAY_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO5699323.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8381..9433
                     /gene="eutR"
                     /locus_tag="ACHKAY_000594"
     CDS             8381..9433
                     /gene="eutR"
                     /locus_tag="ACHKAY_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO5699324.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9439..10338)
                     /gene="hemF"
                     /locus_tag="ACHKAY_000595"
     CDS             complement(9439..10338)
                     /gene="hemF"
                     /locus_tag="ACHKAY_000595"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO5699325.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10342..11211)
                     /gene="amiA"
                     /locus_tag="ACHKAY_000596"
     CDS             complement(10342..11211)
                     /gene="amiA"
                     /locus_tag="ACHKAY_000596"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO5699326.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11425..11850
                     /gene="ypeA"
                     /locus_tag="ACHKAY_000597"
     CDS             11425..11850
                     /gene="ypeA"
                     /locus_tag="ACHKAY_000597"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO5699327.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11837..12286
                     /gene="yfeZ"
                     /locus_tag="ACHKAY_000598"
     CDS             11837..12286
                     /gene="yfeZ"
                     /locus_tag="ACHKAY_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO5699328.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12347..12922
                     /gene="yfeY"
                     /locus_tag="ACHKAY_000599"
     CDS             12347..12922
                     /gene="yfeY"
                     /locus_tag="ACHKAY_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO5699329.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13018..13917
                     /gene="yfeX"
                     /locus_tag="ACHKAY_000600"
     CDS             13018..13917
                     /gene="yfeX"
                     /locus_tag="ACHKAY_000600"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO5699330.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13975..15279)
                     /gene="pbp4b"
                     /locus_tag="ACHKAY_000601"
     CDS             complement(13975..15279)
                     /gene="pbp4b"
                     /locus_tag="ACHKAY_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO5699331.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15284..16708)
                     /gene="murP"
                     /locus_tag="ACHKAY_000602"
     CDS             complement(15284..16708)
                     /gene="murP"
                     /locus_tag="ACHKAY_000602"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO5699332.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16712..17608)
                     /gene="murQ"
                     /locus_tag="ACHKAY_000603"
     CDS             complement(16712..17608)
                     /gene="murQ"
                     /locus_tag="ACHKAY_000603"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO5699333.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17772..18629
                     /gene="murR"
                     /locus_tag="ACHKAY_000604"
     CDS             17772..18629
                     /gene="murR"
                     /locus_tag="ACHKAY_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO5699334.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18758..19549
                     /gene="ucpA"
                     /locus_tag="ACHKAY_000605"
     CDS             18758..19549
                     /gene="ucpA"
                     /locus_tag="ACHKAY_000605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO5699335.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19720..20736
                     /gene="cysP"
                     /locus_tag="ACHKAY_000606"
     CDS             19720..20736
                     /gene="cysP"
                     /locus_tag="ACHKAY_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO5699336.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20736..21569
                     /gene="cysT"
                     /locus_tag="ACHKAY_000607"
     CDS             20736..21569
                     /gene="cysT"
                     /locus_tag="ACHKAY_000607"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO5699337.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21569..22444
                     /gene="cysW"
                     /locus_tag="ACHKAY_000608"
     CDS             21569..22444
                     /gene="cysW"
                     /locus_tag="ACHKAY_000608"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO5699338.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            22434..23531
                     /gene="cysA"
                     /locus_tag="ACHKAY_000609"
     CDS             22434..23531
                     /gene="cysA"
                     /locus_tag="ACHKAY_000609"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO5699339.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23665..24576
                     /gene="cysM"
                     /locus_tag="ACHKAY_000610"
     CDS             23665..24576
                     /gene="cysM"
                     /locus_tag="ACHKAY_000610"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO5699340.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24579..24947)
                     /gene="yfeK"
                     /locus_tag="ACHKAY_000611"
     CDS             complement(24579..24947)
                     /gene="yfeK"
                     /locus_tag="ACHKAY_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO5699341.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25052..25903
                     /gene="pdxK"
                     /locus_tag="ACHKAY_000612"
                     /gene_synonym="thiJ"
     CDS             25052..25903
                     /gene="pdxK"
                     /locus_tag="ACHKAY_000612"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO5699342.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25945..26454)
                     /gene="crr"
                     /locus_tag="ACHKAY_000613"
     CDS             complement(25945..26454)
                     /gene="crr"
                     /locus_tag="ACHKAY_000613"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO5699343.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26495..28222)
                     /gene="ptsI"
                     /locus_tag="ACHKAY_000614"
     CDS             complement(26495..28222)
                     /gene="ptsI"
                     /locus_tag="ACHKAY_000614"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO5699344.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28267..28524)
                     /gene="ptsH"
                     /locus_tag="ACHKAY_000615"
     CDS             complement(28267..28524)
                     /gene="ptsH"
                     /locus_tag="ACHKAY_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO5699345.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28908..29879)
                     /gene="cysK"
                     /locus_tag="ACHKAY_000616"
     CDS             complement(28908..29879)
                     /gene="cysK"
                     /locus_tag="ACHKAY_000616"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO5699346.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30064..30825)
                     /gene="cysZ"
                     /locus_tag="ACHKAY_000617"
     CDS             complement(30064..30825)
                     /gene="cysZ"
                     /locus_tag="ACHKAY_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO5699347.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31055..32041
                     /gene="zipA"
                     /locus_tag="ACHKAY_000618"
     CDS             31055..32041
                     /gene="zipA"
                     /locus_tag="ACHKAY_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO5699348.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32112..34127
                     /gene="ligA"
                     /locus_tag="ACHKAY_000619"
     CDS             32112..34127
                     /gene="ligA"
                     /locus_tag="ACHKAY_000619"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO5699349.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34129..34347
                     /gene="ypeB"
                     /locus_tag="ACHKAY_000620"
     CDS             34129..34347
                     /gene="ypeB"
                     /locus_tag="ACHKAY_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO5699350.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34344..35342)
                     /gene="yfeH"
                     /locus_tag="ACHKAY_000621"
     CDS             complement(34344..35342)
                     /gene="yfeH"
                     /locus_tag="ACHKAY_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO5699351.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            35432..36358
                     /gene="yfeR"
                     /locus_tag="ACHKAY_000622"
     CDS             35432..36358
                     /gene="yfeR"
                     /locus_tag="ACHKAY_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5699352.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36397..37161)
                     /gene="yfeN"
                     /locus_tag="ACHKAY_000623"
     CDS             complement(36397..37161)
                     /gene="yfeN"
                     /locus_tag="ACHKAY_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO5699353.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37410..38243
                     /gene="xapA"
                     /locus_tag="ACHKAY_000624"
                     /pseudo
     CDS             37410..38243
                     /gene="xapA"
                     /locus_tag="ACHKAY_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
     gene            38303..39559
                     /gene="xapB"
                     /locus_tag="ACHKAY_000625"
     CDS             38303..39559
                     /gene="xapB"
                     /locus_tag="ACHKAY_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO5699354.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            39612..39770
                     /locus_tag="ACHKAY_000626"
     CDS             39612..39770
                     /locus_tag="ACHKAY_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO5699355.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            39811..40695
                     /gene="xapR"
                     /locus_tag="ACHKAY_000627"
     CDS             39811..40695
                     /gene="xapR"
                     /locus_tag="ACHKAY_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO5699356.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(40804..40929)
                     /locus_tag="ACHKAY_000628"
     ncRNA           complement(40804..40929)
                     /ncRNA_class="other"
                     /locus_tag="ACHKAY_000628"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(40961..41036)
                     /locus_tag="ACHKAY_000629"
     tRNA            complement(40961..41036)
                     /locus_tag="ACHKAY_000629"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41001..41003),aa:Lys,seq:ttt)
     gene            complement(41041..41116)
                     /locus_tag="ACHKAY_000630"
     tRNA            complement(41041..41116)
                     /locus_tag="ACHKAY_000630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41081..41083),aa:Val,seq:tac)
     gene            complement(41163..41238)
                     /locus_tag="ACHKAY_000631"
     tRNA            complement(41163..41238)
                     /locus_tag="ACHKAY_000631"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41203..41205),aa:Val,seq:tac)
     gene            complement(41283..41358)
                     /locus_tag="ACHKAY_000632"
     tRNA            complement(41283..41358)
                     /locus_tag="ACHKAY_000632"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41323..41325),aa:Val,seq:tac)
     gene            41617..43032
                     /gene="gltX"
                     /locus_tag="ACHKAY_000633"
     CDS             41617..43032
                     /gene="gltX"
                     /locus_tag="ACHKAY_000633"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO5699357.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(43084..43476)
                     /gene="yfeD"
                     /locus_tag="ACHKAY_000634"
     CDS             complement(43084..43476)
                     /gene="yfeD"
                     /locus_tag="ACHKAY_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5699358.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(43478..43837)
                     /gene="yfeC"
                     /locus_tag="ACHKAY_000635"
     CDS             complement(43478..43837)
                     /gene="yfeC"
                     /locus_tag="ACHKAY_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO5699359.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            44058..44133
                     /locus_tag="ACHKAY_000636"
     tRNA            44058..44133
                     /locus_tag="ACHKAY_000636"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44091..44093,aa:Ala,seq:ggc)
     gene            44173..44248
                     /locus_tag="ACHKAY_000637"
     tRNA            44173..44248
                     /locus_tag="ACHKAY_000637"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44206..44208,aa:Ala,seq:ggc)
     gene            44456..46645
                     /gene="pdeA"
                     /locus_tag="ACHKAY_000638"
     CDS             44456..46645
                     /gene="pdeA"
                     /locus_tag="ACHKAY_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO5699360.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(46695..47897)
                     /gene="nupC"
                     /locus_tag="ACHKAY_000639"
     CDS             complement(46695..47897)
                     /gene="nupC"
                     /locus_tag="ACHKAY_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO5699361.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            48233..49471
                     /gene="mntH"
                     /locus_tag="ACHKAY_000640"
     CDS             48233..49471
                     /gene="mntH"
                     /locus_tag="ACHKAY_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO5699362.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(49612..49938)
                     /gene="ypeC"
                     /locus_tag="ACHKAY_000641"
     CDS             complement(49612..49938)
                     /gene="ypeC"
                     /locus_tag="ACHKAY_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO5699363.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(50053..51309)
                     /gene="yfeO"
                     /locus_tag="ACHKAY_000642"
     CDS             complement(50053..51309)
                     /gene="yfeO"
                     /locus_tag="ACHKAY_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO5699364.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            51513..52478
                     /gene="glk"
                     /locus_tag="ACHKAY_000643"
     CDS             51513..52478
                     /gene="glk"
                     /locus_tag="ACHKAY_000643"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO5699365.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            52697..53023
                     /gene="fryB"
                     /locus_tag="ACHKAY_000644"
     CDS             52697..53023
                     /gene="fryB"
                     /locus_tag="ACHKAY_000644"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO5699366.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            53045..54292
                     /gene="fryC"
                     /locus_tag="ACHKAY_000645"
     CDS             53045..54292
                     /gene="fryC"
                     /locus_tag="ACHKAY_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO5699367.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            54307..55392
                     /gene="ypdF"
                     /locus_tag="ACHKAY_000646"
     CDS             54307..55392
                     /gene="ypdF"
                     /locus_tag="ACHKAY_000646"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5699368.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            55392..56429
                     /gene="ypdE"
                     /locus_tag="ACHKAY_000647"
     CDS             55392..56429
                     /gene="ypdE"
                     /locus_tag="ACHKAY_000647"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5699369.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            56454..58949
                     /gene="ptsP"
                     /locus_tag="ACHKAY_000648"
     CDS             56454..58949
                     /gene="ptsP"
                     /locus_tag="ACHKAY_000648"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5699370.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(58952..59809)
                     /gene="ypdC"
                     /locus_tag="ACHKAY_000649"
     CDS             complement(58952..59809)
                     /gene="ypdC"
                     /locus_tag="ACHKAY_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5699371.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(59822..60556)
                     /gene="ypdB"
                     /locus_tag="ACHKAY_000650"
     CDS             complement(59822..60556)
                     /gene="ypdB"
                     /locus_tag="ACHKAY_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO5699372.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(60571..62268)
                     /gene="ypdA"
                     /locus_tag="ACHKAY_000651"
     CDS             complement(60571..62268)
                     /gene="ypdA"
                     /locus_tag="ACHKAY_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO5699373.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            62645..63883
                     /gene="alaC"
                     /locus_tag="ACHKAY_000652"
     CDS             62645..63883
                     /gene="alaC"
                     /locus_tag="ACHKAY_000652"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO5699374.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(63948..64019)
                     /gene="ypdK"
                     /locus_tag="ACHKAY_000653"
     CDS             complement(63948..64019)
                     /gene="ypdK"
                     /locus_tag="ACHKAY_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO5699375.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(64375..65295)
                     /gene="lpxP"
                     /locus_tag="ACHKAY_000654"
     CDS             complement(64375..65295)
                     /gene="lpxP"
                     /locus_tag="ACHKAY_000654"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO5699376.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            65648..65890
                     /gene="yfdY"
                     /locus_tag="ACHKAY_000655"
     CDS             65648..65890
                     /gene="yfdY"
                     /locus_tag="ACHKAY_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO5699377.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(65967..66194)
                     /locus_tag="ACHKAY_000656"
     CDS             complement(65967..66194)
                     /locus_tag="ACHKAY_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699378.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
BASE COUNT        15635 a        16551 c        18038 g        16066 t
ORIGIN      
        1 accaccatga ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg gacgtttgtc
       61 cgtctgcgtc gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aaccgatggc
      121 gcacgacgaa caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac
      181 gcctgccgcg acggaatctc cgctgtggct ggggggggat ctgggcacct gcgatgtggt
      241 gtcgatggtt gttgaccgcg acggtcagcc ggtggcggta tgcctcgact gggccgatgt
      301 ggtccgcgac ggcatcgtct gggatttctt cggtgcggtc accattgttc gtcgccatct
      361 cgacactctt gagcagcaat tcggtcttcg ttttagccat gcggcgactt catttcctcc
      421 aggtacggac ccgcgtattt ccatcaacgt gctggaatct gccgggctgg aagtgagcca
      481 tgtgctggat gagccaaccg cagtcgcgga tctgctgcaa ctggacaacg ccggtgtagt
      541 ggatatcggc ggcggcacta ccggcattgc catcgtgaaa aagggcaagg tgacgtactc
      601 ggcggatgaa gccaccggcg gacatcacat ctctctgact ctcgccggaa atcgtcgtat
      661 ttcgctggaa gaggcagagc agtacaagcg cggtcatggt gacgagattt ggcccgcggt
      721 gaaaccggtg tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat
      781 tactgattta tggctggcgg gcggctcttg tatgcaaccg ggcgtggcgg aactgtttcg
      841 caaacaattc ccggcgttac aggtgcattt accgcagcac agtttgttta tgaccccgct
      901 ggcgatcgcc agtagtgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga
      961 ccgcgctctt tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc
     1021 caccggtgac gctttgcggt ccgggctcgg tgagcagttg cggacagcaa gcgcaaacgc
     1081 gtgggctgaa acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg
     1141 ccgggctgac gcgtagcctg gccgttaaag gtatcgccat gacgctctgg ccatgtccgg
     1201 tgggcgaacc gtgcatcacc gacgtgtgtg cagccgtggc gcagttgcgt gagtcaggct
     1261 gtgatggggt gatcgcattt ggcggcggct cggtgctgga tgcggcgaaa gccgtggcgt
     1321 tgctggtgac gaacccggat agcacgctgg cagagatgtc agaaaccagc gttctgcaat
     1381 cgcgcctgcc gttgattgcc attcccacta ccgccggaac cggctctgaa accaccaacg
     1441 taacggtgat tatcgatgcg gtgagcgggc gtaagcaggt gttagcccat gcctcgctga
     1501 tgccggatgt ggcgattctc gacgccgcat tgaccgaagg tgtgccgtcg catgtcacgg
     1561 cgatgaccgg cattgatgcg ttaacccatg ccattgaagc atacagcgcc ctgaacgcta
     1621 caccgtttac cgacagtctg gcgattggtg ccattgcgat gattggcaaa tcgctgccga
     1681 aagcggtggg ctacggtcat gaccttgccg cgcgcgagag catgttactg gcttcatgta
     1741 tggcgggaat ggcgttttcc agtgcggggc ttgggttgtg ccacgcgatg gcgcatcagc
     1801 caggggcggc gctgcatatt ccacacggtc tggcgaacgc catgttgctg ccaacggtga
     1861 tggaatttaa ccggatggtt tgtcgcgaac ggtttagtca gattggtcgg gcactgcgaa
     1921 ctaaaaaatc cgacgatcgt gacgctatta acgcggtaag tgagctgatt gcggaagttg
     1981 ggattggtaa acgactgggc gatgttggcg cgacatctgc gcattacggt gcatgggcgc
     2041 aggccgcgca ggaagatatt tgtctgcgca gcaacccgcg taccgccagc ctggagcaga
     2101 ttgtcggcct gtacgcagcg gcgcaataaa tgccggatgc gacgcttgcc gcgtctgatc
     2161 cggccttcgg gattgcacat gtagggcgga taaggcgttt acgccgcatc cgccaataaa
     2221 taacaactaa cagggagtaa aggcgatggg aattaacgaa atcatcatgt acatcatgat
     2281 gttctttatg ctgatagctg ccgtagacag gatcctgtcg cagttcggcg gttctgctcg
     2341 tttcctcggt aagttcggta aaagtatcga aggatcaggc ggtcagttcg aagaaggctt
     2401 tatggcaatg ggcgcactgg gcctggcgat ggtcggtatg accgcgctgg caccggtact
     2461 ggctcacgta ctcgggccgg taattattcc ggtttacgaa atgctcggcg ctaacccatc
     2521 gatgttcgcc ggaacactgc tggcgtgcga tatgggcggc ttcttcctcg ccaaagagct
     2581 ggcgggcggc gacgtagcag cgtggctata ctctgggtta attctcgggt cgatgatggg
     2641 gccaacgatt gtgttttcca ttccggtggc gctcggcatt atcgaacctt ctgaccgtcg
     2701 ttatctggcg ctcggcgtgc tggcgggcat tgtgaccatt ccgattggct gtattgctgg
     2761 tggtctggtt gctatgtact ccggtgtgca gatcaacggt cagccggtgg aattcacttt
     2821 cgccctgatc ctgatgaaca tgatcccggt gatcattgtc gcgattctgg tggcgctggg
     2881 gctgaaattc atcccggaaa aaatgatcaa cggcttccag atcttcgcca aattcctcgt
     2941 ggcattgatc accctcggtc ttgccgctgc ggtagtgaaa ttcctccttg gctgggaact
     3001 gatccccgga ctggatccta tctttatggc ccctggcgat aaacccggtg aagtgatgcg
     3061 cgccattgaa gttatcggtt ctatctcctg cgtgctgtta ggggcgtatc caatggtgct
     3121 gctgctgact cgctggtttg aaaaaccgct gatgagcgtc ggtaaagtgc tgaatatgaa
     3181 caacatcgcg gcagccggca tggtggcaac gcttgccaac aacatcccga tgttcggcat
     3241 gatgaagcag atggataccc gcggcaaagt catcaactgc gccttcgccg tttccgctgc
     3301 tttcgccctg ggcgaccact taggcttcgc cgctgccaac atgaacgcca tgatcttccc
     3361 gatgattgtc ggcaagttga tcggcggcgt aacggcgatt ggcgtggcga tgatgctggt
     3421 gccaaaagaa gacgcgaccg cgactaaaac cgaagcggag gcacaatcgt gaacactcgc
     3481 cagctattga gcgtcggtat cgatatcggc accaccacca cccaggtgat tttctcccac
     3541 ctggagctgg ttaaccgtgc ggcggtgtcg caggtgccgc gctacgaatt cattaaacgc
     3601 gaaattagct ggcaaagtcc ggtgttcttt acccctgtcg ataaacaggg cggtttaaaa
     3661 gaagcggaac tgaaaacctt aatactcgag caatatcagg ctgcgggtat tgcgccggaa
     3721 agcgttgatt ctggtgccat catcatcacc ggtgaaagcg cgaaaacccg caatgctcgc
     3781 ccggcggtga tggcgctctc tcaatcgctg ggggattttg tcgttgccag cgccgggccg
     3841 cacctcgaat ccgtgatcgc cggtcacgga gctggggcgc aaaccctttc tgaacaacgg
     3901 ctgtgtcggg tactgaatat cgacatcggc ggtggcaccg cgaactacgc cctgttcgat
     3961 gccggaaaaa tcagcggcac tgcctgtctc aacgtcggtg gtcgcctgat ggaaaccgac
     4021 agccaggggc gcgtggttta cgctcataaa ccggggcaga tgattgtgga tgagtgcttc
     4081 ggtgcaggca ctgacgcccg ttcgctgacc ggcgcgcagc tggtgcaggt tacccggcgg
     4141 atggcggcgc tgattgtcga agtgattgac ggaacgcttt cgccgctcgc gcaggcattg
     4201 atgcaaaccg gtttgctgcc cgcaggtgtt acgcccgaaa tcattacgct ttctggaggc
     4261 gtgggcgaat gttatcgcca ccagcccgcc gacccgttct gttttgccga tattggccca
     4321 cttctggcaa cggcgctgca tgaccatccg cgcctgcgtg agatgaatgt gcagtttccg
     4381 gcgcaaaccg tacgcgccac ggtgattggc gcgggtgcac ataccctttc gctctctggc
     4441 agcacaatct ggctggaggg cgtacaactg ccgctgcgca atttgccggt ggcgatcccg
     4501 attgatgaaa cggatctggt gagtgcctgg caacaggcgc tgcttcagct ggatcttgat
     4561 cccaaaactg acgcgtacgt gctggcgctt cccgcctcgc tgcctgtgcg ttacgccgcg
     4621 gtactgacgg tcatcaacgc gctggtcgat ttcgtcgcgc gttttccgaa tccgcatccc
     4681 ctgctggtgg tggccgggca ggactttggt aaagctctgg gcatgttgtt gcgcccacag
     4741 ctacaacaac tcccgttggc agtcattgac gaagtgattg tccgcgcggg ggactatatc
     4801 gacattggta cgcctctttt tggcggatcg gttgtgccgg tgacggtgaa atcactcgca
     4861 tttccttcct gagggaacga cttatgaaac taaagaccac attgttcggc aatgtatatc
     4921 agtttaagga tgtaaaagag gtgctggcta aagccaacga actgcgttcg ggggatgtgc
     4981 tggcgggcgt tgcagcggca agctcacagg agcgcgtggc ggcaaagcag gtgttgtcgg
     5041 aaatgaccgt agcggacatc cgcaataatc cggtgattgc ctatgaagat gactgcgtga
     5101 cgcggctgat tcaggacgat gttaacgaaa cggcctacaa ccagattaaa aactggagca
     5161 tcagcgaact gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac attgccttta
     5221 cccgcaaagg gctgacctcg gaagtggtcg cggcggtagc gaagatttgc tccaacgcgg
     5281 acctgatcta cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc accattggta
     5341 ttccgggcac cttcagcgcc cgtttgcagc cgaacgatac ccgtgacgac gtgcaaagta
     5401 tcgctgcgca aatctatgaa gggctttcct ttggggtggg cgatgcggtg atcggcgtta
     5461 acccggtaac tgacgacgtg gaaaacttaa gccgcgtgtt ggacaccatt tacggcgtga
     5521 tcgacaaatt caacatccca actcagggct gcgtactggc gcacgtcacc acccagatcg
     5581 aagcgattcg tcgcggcgcg cctggcggac tgattttcca gagtatctgt ggcagcgaaa
     5641 aagggctgaa agagtttggc gtggaactgg cgatgctcga cgaagcgcgc gcagtgggtg
     5701 cagagttcaa ccgtatcgcc ggggaaaact gcctctactt cgaaaccgga caaggctctg
     5761 cgctatccgc tggcgctaac ttcggcgctg accaggtgac gatggaagca cgtaactatg
     5821 ggctggcgcg tcattacgat ccgtttatcg tcaacaccgt ggtcggcttt attgggccgg
     5881 agtatctcta caacgaccgc cagattatcc gtgctggctt agaagatcac tttatgggca
     5941 agctgagcgg catctctatg ggctgtgact gctgttacac caaccacgct gacgctgacc
     6001 agaacctcaa cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac tacatcatgg
     6061 ggatgccgct gggtgatgac atcatgctca actaccagac caccgcattc cacgataccg
     6121 ccactgtgcg tcagttactc aacctgcgtc cgtcaccgga gtttgaacgc tggctggaaa
     6181 gcatgggcat tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg tcactgttct
     6241 tctgatgacg cggagataac acatcatgga tcaaaaacag attgaagaaa ttgtacgcag
     6301 cgtgatggcg tcaatgggac aaacggcccc cgcgccgtca gaagcaaagt gcgccaccac
     6361 caactgtgcg gcaccggtga cctcggaaag ctgcgcgctg gatttaggtt ctgcggaagc
     6421 aaaagcgtgg attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgtcg
     6481 cagcaccgtg gcccgcgttt gtaccggtcg tgccggtccg cgtccgcgta cgcaggcgtt
     6541 gctgcgtttc ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga
     6601 agagtgggtg aaagcgcagg gcttgctgga agtacgctcc gaaattagcg acaaaaatct
     6661 ctacctgact cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa
     6721 agcacagtgt gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga
     6781 tgccatcacc gtgaactacg aagagatcct gccgccgctg atggcgggcc tgaaacaggc
     6841 cgggctgaaa gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca
     6901 gatcggtgag atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt
     6961 agggcagtca gaaagcctct cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt
     7021 tgaggccgat cgcacctgta tctctaacat tcaccagggc ggcacgccgc cggttgaagc
     7081 cgcagccgtc attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa
     7141 catgacccga taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc
     7201 catgcgggtg attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca
     7261 tattcgtagc ctcgggctga tttctgccga ttctgacgac gtcacctaca ttgcggctga
     7321 cgaagcgacc aaacaagcga tggtcgaagt ggtatatggt cgctcgctgt atgcgggagc
     7381 ggcacacggc ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc
     7441 ggcggaagtg cgtgccggtc tggatgcgat ggttgcacat attgaaaatg gcgcagcttt
     7501 ccagtgggcc aatgacgcag aaaacaccgc cttcctggca catgtagttt cgcgtaccgg
     7561 ttcttatctc tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc
     7621 accgccgctg gaagcgacct acggcattga tgcagcgttg aaatctgccg acgtacagct
     7681 ggtgacctat gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag
     7741 ccaggccgcg tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg
     7801 taatccaatc cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga
     7861 agtggacggc atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt
     7921 tcgtctgctc agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga
     7981 tctggcggcg tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg
     8041 tgtcatcagc cgcaaggaga tcggtcggcc agacgatgac acccagtggc tggttactgg
     8101 ctttaaccgc cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga
     8161 atctgctgac gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga
     8221 agtggctgcc cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt
     8281 ttctgccggg acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt
     8341 cggaggtgcc gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc
     8401 caatttgcac catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt
     8461 cgaagtggat aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat
     8521 taccgcctgg cagcagattt acgatcagct gcatccgggc aagtttcatt gtgaatttac
     8581 ggaaattcta ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca
     8641 gtcgtgcctg gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca
     8701 gggatttatc ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac
     8761 tgaatttgaa ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga
     8821 tgtcatcacc cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg
     8881 tagccagtcg gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca
     8941 acaggcgctg gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa
     9001 agtgctgggg gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat
     9061 ggtgacggcg gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga
     9121 atatgtgctg gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca
     9181 tgtcagtcgc cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc
     9241 gtggctgaaa cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca
     9301 aagcacaacg gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgc
     9361 cacggattac cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg
     9421 ggaatggggg tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc
     9481 ttctgggctg ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga
     9541 catcaggatc gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca
     9601 gaccagattg aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc
     9661 gccgtaggcc atcgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt
     9721 gcctaccgcc tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa
     9781 gaacagcccg ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgtcgca
     9841 ccactttttg taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt
     9901 gcggtgccag tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc
     9961 gccaaaccac cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc
    10021 gtggctggtg ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc
    10081 gaaactgcgc ccggcaagtt ccgggcgatg agcggtggcg gaagctggca tcgcctcgcc
    10141 gtggacgtga gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg
    10201 actacgcccg ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc
    10261 gacggcggtc agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg
    10321 gtgtgcgtcg ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca
    10381 gtggaaataa ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc
    10441 cgtgcctaac agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga
    10501 aggaaccgac ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag
    10561 tttatgcacc ggcttaatct ttttcagaat atgcgagccg agcgtcaggc tgtttttaat
    10621 agtatcggtt tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc
    10681 ctttttaccg gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc
    10741 gctacttgcc ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt
    10801 aaagccatcg gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac
    10861 gcgatcgtaa agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg
    10921 attacgcaaa atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga
    10981 accgttgcgt ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac
    11041 acgtttgccg ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg
    11101 ttcgtctttg gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt
    11161 cagcacctgg cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga
    11221 gttgtaataa taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga
    11281 tgttgagaat tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt
    11341 tcaaccgatt ttcgtcagat attggctaat tcgaccgttc acgccataat cagcctttta
    11401 acccgcaaaa aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa
    11461 gaggtcatca ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg
    11521 gacatcgagc gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtaaacggt
    11581 gaagtggtcg gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt
    11641 ggcgtgcatc cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag
    11701 aaaaagctga ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac
    11761 atggtgctcg gaatgtatga acgcctggga tatgaacacg ccgacgtgct gagtctgggt
    11821 aagcgtttga ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg
    11881 acgcctgcgt ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt
    11941 gctgtttgtc attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta
    12001 tcccgatcac gataatttct ggctggggct gattccaggc attccggcgg tgctggcgtt
    12061 tttgttgagc ggtcggcggg ctacatttcc tcgcacttgg catgtgctct attttctgtt
    12121 gctgttggcg caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc
    12181 cgttagcggt atcggtctgg cgctggtagt ggcggatatt gtggcgttaa tctggctgct
    12241 gaccaatcga cgtttacgcg cttgttttaa tgaagaaaaa gaataacggc actttttggt
    12301 gaatttgcac tccaagcaac gttattgaat aaccaaagga agtgacatga aatcgctgcg
    12361 tttaatgtta tgcgctatgc cgttgatgct gaccggctgt tccacgatgt cgtcagttaa
    12421 ctggtcggcc gctaacccgt ggaactggtt tgggtcgtcc accaaagtga gcgagcaggg
    12481 cgttggtgaa ttaacggcgt ccacaccact gcaagaacaa gccattgccg atgcgcttga
    12541 tggcgattat cgcctgcgca gcggaatgaa aaccgcgaac ggcaacgtgg tgcgcttttt
    12601 tgaagtgatg aagggcgaca acgtggcgat ggtgattaac ggcgatcaag gcacgatcag
    12661 tcgcattgac gtgctggata gcgatattcc tgctgacacc ggtgttaaaa tcggtacacc
    12721 gtttagcgac ctttacagca aagcatttgg caattgccaa aaagctgatg gtgatgataa
    12781 tcgtgccgtc gaatgtaaag ccgaaggcag tcaacatatt agctaccagt tcagcgggga
    12841 atggagtggt cctgaagggt taatgccttc ggacgatacc ctgaaaaact ggaaagtcag
    12901 taaaattatc tggcggcgtt aatttgcgtc tgaacaaacc gccgccgcga aaaaacacgt
    12961 aaaatagcgc tcaacaatgc cacggattgc gtggcattat tattttcagg aggaacaatg
    13021 tctcaggttc agagtggcat tttgccagaa cattgccgcg cggcgatttg gatcgaagcc
    13081 aacgtgaaag gggaagttga cgccctgcgt gcggccagta aaacatttgc cgacaaactg
    13141 gcaacttttg aagcgaaatt cccggacgcg catcttggtg cggtggttgc ctttggtaac
    13201 aacacctggc gcgctctgag cggcggcgtt ggggcagaag agctgaaaga ttttccgggc
    13261 tacggtaaag gccttgcgcc gaccacccag ttcgatgtgt tgatccacat tctttctctg
    13321 cgtcacgacg taaacttctc tgtcgcccag gcggcgatgg aagcctttgg tgactgcatt
    13381 gaagtgaaag aagagatcca cggcttccgt tgggttgaag agcgtgacct gagcggcttt
    13441 gttgacggta cggaaaaccc ggcgggtgaa gagacgcgtc gcgaagtggc ggttatcaaa
    13501 gacggcgtgg atgcgggcgg cagctatgtg tttgtccagc gttgggaaca caacctgaag
    13561 cagctcaacc ggatgagcgt tcacgatcag gagatgatga tcgggcgcac caaagaggcc
    13621 aacgaagaga tcgacggcga cgaacgtccg gaaacctctc acctcacccg cgttgatctg
    13681 aaagaagatg gcaaagggct gaagattgtt cgccagagcc tgccgtacgg cactgccagt
    13741 ggcactcacg gtctgtactt ctgcgcctac tgcgcgcgtc tgcataacat tgagcagcaa
    13801 ctgctgagca tgtttggcga taccgatggt aagcgtgatg cgatgttgcg tttcaccaaa
    13861 ccggtaaccg gcggctatta tttcgcaccg tcgctggaca agttgatggc gctgtaagcg
    13921 tttctgattc attgattaaa ggccaggcag taacattact gactggcctt tttattactt
    13981 ttgctttaac gccgcataca cctgatcaac tacccaacca tacgttgcaa tcggcagctg
    14041 accgctttcg aacatattgg gattcttttg cggatcggca accggcgaat gtggcttgtt
    14101 gcttaacatc acaatcgcca tatgattcac cggatcgata acggtcaccg ttccggtcca
    14161 gccagtgtga ccgtaggttt gcgggcttgc cagcgtgcca aacgtcggcg tcatggtggc
    14221 attaccattc acgcgccagc cgaggccaaa agtggcatct tccttagagc tggtggtgaa
    14281 catcttcacc gtttccgcac tgaacagctg cacatcgcca tagcccccgc cgttcagcat
    14341 cgtttgcatt aacaccgcaa tatcgccggt attggaaaac aaacctgcgt gcccggaaac
    14401 gccgcccatc gaataaaagg ctttttcatc gtgcacctga ccccagagag tggaggtgcg
    14461 gatattcggg aaatggatca cgccatcgcg ggtattgccg ttcaattccg tggcggcaat
    14521 ttgctgcggt ttaaagcctt tcagcagcgg gttaaatacc gtatgtgtca ggccgagcgg
    14581 gcgataaatc gactcttcaa catagcggtc aagcggctga ccggtgaccg actcgacgat
    14641 aaatccaagc agcatataat cgacatcact gtaaatatgc ttgctgccgg gctgatactc
    14701 cagcggcgtg cgcttgatca tctccagcgt ctggccttta tcctgggaat ataacgcgcc
    14761 cgcgaccgct ttattcggat attgtgggtc cgccgggaaa ccgccgctgt gatgcagcag
    14821 gtcagaaatc cgcagcgtgt ttttgccttt gatggtgtca tttggactat cggcaaaccc
    14881 cggaatatat ttcgcaatcc gatcgtcagg atgcagtttg ccttcgctca tcagcttttg
    14941 cagggcgaag ttcgtggcgt acattttggt gtttgaggcc aggtcataca gcgtcccggt
    15001 ggtggctttg acaggctgtt ccatcaacac gctgccatcg tactttttcg ccgctcccca
    15061 ggctttgcga tacacaatct gattatcttt aatgatcagc aggtttacgc cgggataacc
    15121 ggcgtccact tgctggctaa tccagcgatc catctggtta agccgttcga cgttgaaccc
    15181 tgctttctct ggcgaacttt ctgtcagaac agggtatttt gcggcgctga cgctacaact
    15241 aacagccagc agagaaagat agagcattgt ccgtttcatg atatcagtcc agattgacgt
    15301 tacggcagcc aaagagcgtg gtgaaaataa acccgcaaac ccatgccacc agaatcccgc
    15361 cagcataaac cgccattgcc ggaaggatgc cttgtgcgga agtcatcagc ggcagcgcca
    15421 ccagaccaga cggcccaaag gcgctgttta agcccatcgg cagaccccac caggctatca
    15481 ggccgataaa caaaccgccc gctgcgccgc ctaaacaggc ggtaataaac ggtttcatgc
    15541 ggggcagggt gacgccgtaa atcaacggtt caccaacgcc cagcagaccg ggaataatcg
    15601 ccccgcgtac ctgactgcgt aatgcactgt gcggttgcgc ccgccagtag agtgccagcg
    15661 ccgcgcccac ctggcccgcg cctgccattg aaaggatggg aaataagctg ttgaatccct
    15721 ggctgtccat taacgcgagg taaacaggaa taaagccctg atgcacgcca aataccacgg
    15781 cgatcaggaa cagcccggct aaaaccgcac aaccgaacgg attactgttc aggtgcataa
    15841 acagccatga cataccttcg aataaccagc cacccagcgg cataatgatc aggtacgcaa
    15901 gcgtagcggt gatcagcagg gtgattaacg aggtcagcag catgtcgaga tcgtccggca
    15961 taaagcggcg caccatgcct tcaatgcggg cgcaggccca cgcggcaatc agcacgccga
    16021 taatattgcc gcgcggatcg atgggcagac caaagaaatc gtgaaaaccg gcgtagtaac
    16081 cggtggttgc agcggggtta taaccgagca aaaagagtgc ggcaataatt gcgccattga
    16141 cgcccgtgcc gccaaatgcc tgggcggcgt tatagcccac cagaatcacc aggaaagtga
    16201 acaaaccttt gctgaacacc ttcataaaat tcagcgcgtc gggtagcgtg ccctgagcat
    16261 ctgccggaac gtgcatcacc gtggcaatta acgtcgctat ccccagcagc agaccggcgg
    16321 caataaaacc ggggatcagc ggcgtgaaga tggtggcgaa tttggcgaga aattgttgca
    16381 cgccggaggt ttgtttggct tttaactgac gtttgttctg ggcggcgatt tctgcggcat
    16441 cctgtaccgg tgcctcgccc agcagctcgc tcatcgcttt tgcggcacga tgcgctttac
    16501 caggcccaaa aacgacctgc acctggtcgc tggtcaaaat gacgcccttc acgccttcca
    16561 gcgttttgat attgggatca accagtgaac tgtcatgtac acccagacgc aggcgcgtca
    16621 tacagttacc acaactggcg atatttcccg gtccgccgac acgggtaaga atggtgttca
    16681 gaagttcact gctgatctct ttggccatgg gttattcctt gtctaaaacc tgacgaataa
    16741 agccgccgtg ttgatccagg cgttttttag cttctgcggc atcgagattt ttcagcacca
    16801 tcacaatggc cgttttacag ttgcgctcgc aggcaattaa cgccgcttcc gcttgttcgg
    16861 cattacatcc ggtggcgttt ttaacgatat tgacctggcg cacatgcagt ttttcgttgg
    16921 tggcgaccac atcgaccatc aggttgccga acactttgcc agatttaatc atcagcccgg
    16981 tggaaagcat attgagcacc agtttctgcg ctgtgcctgc tttcattcgt gaagaaccag
    17041 tgaccacttc ggcacctaca atcggtgtaa tggcaaactc ggcggtggtg gaaacggcac
    17101 tccccggatt acaggaaatt cccactgtgc ggcagccgag ctggcgtgcg tattccagtc
    17161 cggcaatcac atacggcgtg cgaccgctgg cagcaatgcc aaccaccaca tcctgtgccg
    17221 ttaaattaat atttttcaga tcgttaacac caccttcccg gctatcttca gcgccttcca
    17281 ccgcgtgctg aatggcatat tcgcctccag caatcaaacc aaccaccaga cccggtttca
    17341 cgccgtaggt gggcggacat tcgctggcat ccagaatccc cagacgaccg gatgttcccg
    17401 caccgagata aatcagacga ccgccgccgc tgacctgggc gtggataaca tcgatcgccg
    17461 cggcgatatc cggcaggacg cgctcaacgg caagcggtac ggttttatct tcatcgttga
    17521 taatccggca catttccagc gtcgatacgc ggtcaatttc agccgaggcg gtgttcgagc
    17581 cttcagtaat catcttttct agttgcatcg gtggtgacct tactatttac gatcaaataa
    17641 ttgcagatgt atggaatcat atatcccgaa ttgacagact taaaggaatc attgagtcta
    17701 cactcgcaga gtgtgaagag cgtcataaga acttaagcgg aatttatgct gccagcccag
    17761 gagagtggaa gatgttgtac ctgacgaaaa taagcaatgc ggggagtgaa ttcaccgaaa
    17821 acgagcaaaa aatcgctgac tttctgcagg ccaacgtgag tgagctgcaa tccgtctctt
    17881 cgcgcaagat ggcgaaacag ctcggcatca gccagtcgag tattgtgaag tttgcccaga
    17941 agctcggcgc acaaggattt actgaattgc gtatggcgtt aattggcgaa tacagtgcca
    18001 gccgggagaa aacgaatgcc acggcgctgc acctgcacag ttcgattacc agtgatgatt
    18061 cgctggaagt gattgcccgc aaactgaatc gcgaaaaaga gctggcgctg gagcaaacct
    18121 gcgcgttgtt cgactacgcg cggctgcaaa aaatcattga tgtcatcagt aaagcgcaat
    18181 ttattcagat aacgggcctg ggcggatcgg cgctggtggg gcgcgatctg tcgttcaaac
    18241 tgatgaaaat tggatatcgc gtggcctgcg aagccgacac gcacgtacaa gccaccgttt
    18301 cccaggcgtt gaaaaaaggc gatgtacaaa tcgctatctc ttacagcggc agtaaaaaag
    18361 agattgtctt gtgcgcagaa gcggcacgaa agcagggggc aacggtgatt gccattactt
    18421 ctctggctga ctctccgctg cggcggctgg cgcatttcac gctcgatacc gtttccggtg
    18481 aaaccgaatg gcgtagctcg tcgatgtcta cccgcacggc gcaaaattct gttaccgatt
    18541 tactgtttgt tggtcttgtg caactcaatg atgtcgagtc attgaaaatg attcagcgca
    18601 gcagtgagct aacacaacgc ctgaaatgat gcataaagca gcgactggat tgagattttc
    18661 ctgaattagt gagctgatcc gcagcaatat tttgtttatc ctgtattttc agagggaatg
    18721 gagtgtaacg ctctgtatta acaaggagag cattaaaatg ggtaaactca cgggcaagac
    18781 agcactgatt acgggcgcat tgcagggaat tggcgaagga attgccagaa cttttgcgcg
    18841 tcatggcgca aacctaatct tgctggatat ctcccctgag atcgaaaagc tggcggacga
    18901 actgtgtggt cgtggtcatc gctgtacggc ggttgtcgcc gatgtgcgtg acccggcgtc
    18961 ggtagccgca gctatcaaac gcgcgaagga aaaagaaggg cgcattgata tcctggtgaa
    19021 taacgcaggc gtttgtcgtc tgggcagttt cctcgatatg agcgatgaag atcgcgattt
    19081 ccatattgac atcaatatta aaggcgtatg gaacgtcacg aaggcggtgc tgccagagat
    19141 gattgcgcgc aaagatggtc gcattgtgat gatgtcttca gtcactggtg atatggtggc
    19201 cgatccgggt gaaacggcgt acgccttaac gaaagcggcg attgttggcc tgacaaaatc
    19261 gctggcggtg gagtacgcgc aatccggtat tcgcgttaac gccatctgcc cggggtacgt
    19321 ccgcacgcca atggcggaaa gcattgcccg ccagtcgaac ccggaagatc cagagtcggt
    19381 gctgactgat atggcaaaag caatcccgct gcgtcgcctc gccgatccgc tggaagtcgg
    19441 cgaactggcg gccttcctcg catcggatga atccagctat ttaaccggta cacagaatgt
    19501 gattgatggc ggcagcacac tgccggagac ggttagcgtc ggtatctgat tcacctctgt
    19561 ttcctccctg catgtgtggg gaggatttcg ttttaaaccc ggttcttatt cccttttcaa
    19621 cttccaaatc accaaacggt atataaaacc gttactcctt tcacgtccgt tataaatatg
    19681 atggctatta gaaagtcatt aaatatataa gggtgcgcaa tggccgttaa cttactgaaa
    19741 aagaactcac tcgcgctggt cgcttctctg ctgctggcag gccatgtaca ggcaacggaa
    19801 ctgctgaaca gttcttatga cgtctcccgc gagctgtttg ccgccctgaa cccgccgttt
    19861 gaacagcaat gggccaaaga taacggcggc gacaaactga cgattaaaca atctcatgcc
    19921 gggtcatcaa aacaggcgct ggcgatttta cagggcttaa aagccgacgt tgtcacttat
    19981 aaccaggtga ctgacgtaca aatcctgcat gacaaaggca agctgatccc ggcggactgg
    20041 cagtcgcgcc tgccgaataa tagctcgccg ttctactcca ccatgggctt cctggtgcgt
    20101 aagggcaacc cgaagaatat ccacgactgg aacgacctgg tgcgctccga cgtgaagctg
    20161 attttcccga acccgaaaac gtcgggtaac gcgcgttata cctatctggc ggcatggggc
    20221 gcagcggata aagctgacgg tggcgataaa gccaaaaccg aacagtttat gacccagttc
    20281 ctgaaaaatg ttgaagtgtt cgataccggc ggtcgtggcg caaccaccac ttttgccgag
    20341 cgtggcctgg gcgatgtgct gattagcttt gaatcggaag tgaacaacat ccgtaaacag
    20401 tatgaagcac agggctttga agtggtgatc ccgaaaacca atattctggc ggaattcccg
    20461 gtggcgtggg tcgataaaaa cgtgcaggcc aacggtacgg aaaaagcagc aaaagcctac
    20521 ctgaactggc tctacagccc gcaggcgcag accatcatca ccgactatta ctaccgcgta
    20581 aataacccgg aagtcatgga caaactgaaa gataaattcc cgcagaccga gctgttccgc
    20641 gtggaagaca aatttggctc ctggccggaa gtgatgaaaa cccacttcac cagcggcggc
    20701 gagttagaca agctgttagc ggcggggcgt aactgatgtt tgcagtttca tcaagacgcg
    20761 tgctgccggg ctttacctta agcctcggca ccagtctgct gtttgtgtgc ctgattttgc
    20821 tgctgccgct ctccgcgctg gtgatgcagc tggctcagat gagctgggcg cagtactggg
    20881 aggtgatcac caacccacaa gtggtcgcgg cctacaaagt gacgctgctg tcggcgtttg
    20941 tggcatcgat ttttaacggc gttttcggtc tgctgatggc gtggatttta acacgttatc
    21001 gctttccggg ccgcacgctg cttgatgcgc tgatggattt accctttgcg ctgccaacgg
    21061 cagtcgccgg tttaacgctg gcctcgctct tttccgtaaa cggtttttac ggtgaatggc
    21121 tggcgaagtt tgatatcaaa gtcacctata cctggcttgg tattgcggtg gcgatggcct
    21181 ttaccagcat tccgtttgtg gtgcgtaccg tgcagccggt gctggaagag ttaggcccgg
    21241 aatatgaaga agcggcggaa acgcttggtg caacgcgctg gcagagtttc tgcaaagtgg
    21301 tgctgccgga actttctcca gcgctggtgg cgggcgtggc gttgtcgttt acccgtagtc
    21361 ttggtgagtt tggcgcggtg atatttatcg ccgggaacat cgcctggaag acggaagtga
    21421 cgtcgctgat gatttttgtg cgcttgcagg agtttgatta cccggcggcg agcgcgattg
    21481 cttcggtgat cctcgcggca tccctgctgc tgctgttctc aattaacact ctgcaaagtc
    21541 gctttggtcg gcgtgtggta ggtcattaat ggcggaagtt acccaattga agcgttatga
    21601 cgcgcgcccg attaactggg gcaaatggtt tctaattggc atcgggatgc tggtttcagc
    21661 gttcatcctg ctggtgccga tgatttacat cttcgtgcag gcattcagca aggggctgat
    21721 gccggtttta cagaatctgg ccgatccgga catgctgcac gccatctggc tgacggtgat
    21781 gatcgcgctg attgccgtac cggtaaacct ggtgttcggc attctgctgg cctggctggt
    21841 gacgcgcttt aacttccctg gacgccagtt actgctgacg ctactggaca ttccgtttgc
    21901 cgtatcgccg gtggttgccg gtctggtgta tctgctgttc tacggctcta acggcccgct
    21961 gggcggctgg ctggatgaac ataatctgca aattatgttc tcctggcccg gaatggtgct
    22021 ggtcaccatc tttgtgacgt gtccgtttgt ggtgcgcgag ctggtgccgg tgatgttaag
    22081 ccagggcagc caggaagacg aagcggcgat tttgcttggc gcgtctggct ggcagatgtt
    22141 tcgccgcgtc acattgccga acattcgctg ggcgctgctt tatggcgtgg tgttgaccaa
    22201 cgcccgcgca attggcgagt ttggcgcggt gtcggtggtt tccggctcga ttcgcggcga
    22261 aaccctgtcg ctgccgttac agattgaatt gctggagcag gactacaaca ccgtcggctc
    22321 ctttaccgct gcggcactgt taacgctgat ggcgattatc accctgtttt taaaaagtat
    22381 gttgcagtgg cgcctggaga atcaggataa acgcgcacag caggaggaac atcatgagca
    22441 ttgagattgc caatattaag aagtcgtttg gtcgcaccca ggtgctgaac gatatctcac
    22501 tggatattcc ttcaggtcag atggtcgcgt tgctggggcc gtccggttcc gggaaaacca
    22561 cgctgctgcg cattatcgcc gggctggagc atcaaaccag cgggcatatt cgcttccacg
    22621 gcaccgacgt cagccgcctg cacgcgcgcg atcgcaaggt cggtttcgtg ttccagcatt
    22681 acgcgctgtt ccgtcatatg acggtgttcg acaatatcgc ttttggcctg acggtgctgc
    22741 cacgtcgcga gcgcccgaat gccgcagcca tcaaagcgaa agtgacaaaa ttgctggaga
    22801 tggtgcagct tgctcatctg gcagatcgtt atccggcaca gctttccggc ggccagaaac
    22861 agcgcgtggc gctggcgcgc gcgctggctg tagaaccgca aattctgctg cttgatgaac
    22921 cgtttggcgc gctggatgcg caggtgcgta aagagctgcg tcgctggctg cgtcaactgc
    22981 atgaagagct gaaattcacc agcgtgttcg tgacccacga ccaggaagag gcgaccgaag
    23041 tagccgatcg tgtagtggtg atgagtcagg ggaatattga acaggctgat gcgccgaatc
    23101 aggtatggcg cgaaccagcg acccgttttg tactcgaatt tatgggcgaa gtgaaccgcc
    23161 tgcagggaac cattcgcggc gggcagttcc atgttggcgc gcatcgctgg ccgctgggct
    23221 acacacctgc gtatcagggt ccggtggatc tcttcctgcg cccgtgggaa gtggatatca
    23281 gccgccgtac cagtctcgat tcgccgctgc cggtacaggt actggaagcc agcccgaaag
    23341 gtcactacac ccaattagtg gtgcagccgc tggggtggta caacgaaccg ctgacggtcg
    23401 tgatgcatgg cgacgatgcc ccgcagcgtg gcgagcgttt attcgttggt ctgcaacatg
    23461 cgcggctgta taacggcgac gagcgtatcg aaacccgaga tgaggaactt gctctcgcac
    23521 aaagcgcctg ataggttgag tgaatgttaa acgcccggag gcgcttcccg cgatccgggc
    23581 tttttaatgg caaggtttgt aacctgtaga cctgataaga cgcgcaagcg tcgcatcagg
    23641 caacaccacg tatggataga gatcgtgagt acattagaac aaacaatagg caatacgcct
    23701 ctggtgaagt tgcagcgaat ggggccggat aacggcagtg aagtgtggtt aaaactggaa
    23761 ggcaataacc cggcaggttc ggtgaaagat cgtgcggcac tttcgatgat cgtcgaggcg
    23821 gaaaagcgcg gggaaattaa accgggtgat gtcttaatcg aagccaccag tggtaacacc
    23881 ggcattgcgc tggcaatgat tgccgcgctg aaaggctatc gcatgaaatt gctgatgccc
    23941 gacaacatga gccaggaacg ccgtgcggcg atgcgtgctt atggtgcgga actgattctt
    24001 gtcaccaaag agcagggcat ggaaggtgcg cgcgatctgg cgctggagat ggcgaatcgt
    24061 ggcgaaggaa agctgctcga tcagtttaat aatcccgata acccttacgc ccattacacc
    24121 actaccgggc cggaaatctg gcagcaaacc ggcgggcgca ttacccattt tgtctccagc
    24181 atgggaacaa ccggcactat caccggcgtt tcacgcttta tgcgcgaaca atccaaacca
    24241 gtaacgattg ttggcctgca gccggaagaa ggcagcagta ttcccggcat tcgccgctgg
    24301 cctgcggaat atctgccggg gattttcaac gcttctctgg tggatgaggt gctggatatt
    24361 catcagcgcg atgcagaaaa caccatgcgc gaactggcag tgcgggaagg aatattctgt
    24421 ggcgtcagct ccggcggcgc ggttgccgga gcactgcggg tggcaaaagc taaccctggt
    24481 gcggtggtgg tagcgatcat ctgcgatcgt ggcgatcgct acctttccac cggggtgttt
    24541 ggggaagagc attttagcca gggggcgggg atttaagatt attgcgcagg caccgtttta
    24601 tccgtctgcg caattagcgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg
    24661 atcttcacaa tatacggctt cccggtaatc gatgacgacg aagcaacctt atcaataaac
    24721 tgctcggcag tgtcaatgcg gttacgggta ttgcccagct tcagacgcag atgagaaacc
    24781 gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct
    24841 aatccctcca gcatggcgtt gatgcgggct tcttcatggg cagttaactt cgcgtaagcg
    24901 gggagtgtca ttagtagtgt aatgaccaga caaatgattt tcttcattga attaccttat
    24961 ccttgttttg tgatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg
    25021 atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa
    25081 gagcagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtggttt acggcagcgt
    25141 gggcaacagc attgccgtgc ctgctatcaa acagaacggt ctgaatgttt ttgccgtgcc
    25201 gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga
    25261 tgaatggttt agcggttatt tgcgtgcgct tcaggagcgt gatgcgctgc gccagctgcg
    25321 cgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct
    25381 gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga
    25441 tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact
    25501 gccgctggcg cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa
    25561 ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa
    25621 atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt
    25681 ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg
    25741 gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa
    25801 cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca
    25861 tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg
    25921 cgccattttt cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac
    25981 ggttacgcta ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac
    26041 cggagtcagg gtagacttgg ctttctcttc cagtagcggc agatcaaatt caatgacagt
    26101 atcgccaact ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag
    26161 ttcaacggtg tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga
    26221 gaacgcgtga ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt
    26281 gttacccgtt ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc
    26341 cggcacgtct tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc
    26401 ggtatccttc ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt
    26461 ctcctaagca gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga
    26521 acttgttaac cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta
    26581 acaccttcgc atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa
    26641 tggcgctcat agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat
    26701 cgccagcaag ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa
    26761 cttgcttgat caagttcagc acggacggtg acattggttg gtaaaggtgt gaaatcatat
    26821 cattaccacg gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga
    26881 aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta
    26941 cgccgatttc aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga
    27001 tttcgatctc tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa
    27061 tgcgcaattt accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct
    27121 ctttacgatc catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg
    27181 ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg
    27241 cctgcgagcc acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg
    27301 gcagcgcgtc gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc
    27361 cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat gttagcgcac acttctacct
    27421 ggtgaccgtc cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag
    27481 aagccacttg ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt
    27541 aaacctgatt atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga
    27601 cgctaccggt acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg
    27661 aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg
    27721 tttcggacgg cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt
    27781 cgataatctt caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag
    27841 ccgcacgttc tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga
    27901 taacttcatg agcagctgcg tcagctgtca tgtgcttatc tttaatcagg gctatgattt
    27961 cctgctccag ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt
    28021 cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg
    28081 cacgaccgct cagaaaacgt tcaacttcct gatcaacctg gtcggcagaa atttttttcc
    28141 ggtcaatgac aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg
    28201 atgctaaaat gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc
    28261 gggaaattac tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc
    28321 ttcgccttct gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt
    28381 aaacaggctt ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa
    28441 gcccttagct tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt
    28501 aatggtaact tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa
    28561 ctaaagtcta gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga
    28621 cgcgacgcac gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc
    28681 gtacgtcatt aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc
    28741 agctttggct tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat
    28801 accagttccg acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa
    28861 aaagcaccta aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcagt
    28921 tctttctcag tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgac
    28981 ggtagaataa ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgcc
    29041 gcagcaacag ctgctccaga agagatacct gcaagaatac cttcttcttc catcagacga
    29101 cgcgcggtag aaatcgcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga
    29161 tcgaggttag ctgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta
    29221 atctcttcac cagccagtgc ctgggcgata actggagaat cggttggctc aacggcgaca
    29281 gagataagat cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtcagcgta
    29341 ccgccagtcc caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc
    29401 tccggaccgg tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc
    29461 agcaggtatt tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc
    29521 atgcctttag caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg
    29581 cgttcaatac tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct
    29641 acataggcca gtgcaatccc ggtattaccg ctggtcggtt caaccagttc aacgcctggt
    29701 ttcagcacgc cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg
    29761 ctgaagctgg ggttacgaga ttccaccttc gccagaatgc gtccgttacc gatgcgattc
    29821 aggcgaacca gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg
    29881 gcctgtcctt aactgtatga aattgggata caacaggtag catacccgct cagagaatat
    29941 gcggaagtaa ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga
    30001 ataaggggcg gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga
    30061 ttgttaccgc cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc
    30121 acaaacggca acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt
    30181 taaagcacca aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt
    30241 aaacggcact ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc
    30301 gctaaacagg aaccacagta ccggcgcgac ggtttgccca ataccaggga ttaagtaaag
    30361 aattagcaat acaattgcgc gcggcagata ccaggcaaat ttttgccatt cgcgtttcat
    30421 gattcgcggc acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt
    30481 caatcgtgct tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc
    30541 aatcgtggag aagaaatagc caaacactaa cagcacagag atgacagcca gaggccacaa
    30601 cagataactc agccattgta gccagtccgg aacgtaactc atgagagtcg ggatccagac
    30661 atcgagctgt gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag
    30721 cggtaaaata acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa
    30781 atagtaaaaa ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc
    30841 cttttgacca atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt
    30901 taggatatgt tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag
    30961 ttaatagtgc acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc
    31021 gtggtggcaa ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt
    31081 aatcattgtt ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg
    31141 taaagaacga tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga
    31201 cgacgattct tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca
    31261 ccgcgtgaat catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca
    31321 acaccagtac caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccgcc
    31381 tgaagcgcag gtaccgccgc aacatgctcc gcgtccagcg cagccggtgc agcagcctgc
    31441 ctatcagccg cagcctgaac agccgttgca gcagccagtt tcgccacagg tcgcgccagc
    31501 gccgcagcct gtgcattcag caccgcaacc ggcacaacag gctttccagc ctgcagaacc
    31561 cgtagcggca ccacagcctg agcctgtagc ggaaccggct ccagttatgg ataaaccgaa
    31621 gcgcaaagaa gcggtgatta tcatgaacgt cgcggcgcat cacggtagcg agctaaacgg
    31681 tgaactgctt cttaacagca ttcaacaagc gggcttcatt tttggcgata tgaatattta
    31741 ccatcgtcat cttagcccgg atggcagcgg cccggcgtta ttcagcctgg cgaatatggt
    31801 gaaaccggga acctttgatc ctgaaatgaa ggatttcact actccgggtg tcaccatctt
    31861 tatgcaggta ccgtcttacg gtgacgagct gcagaacttc aagctgatgc tgcaatctgc
    31921 gcagcatatt gccgatgaag tgggcggtgt cgtgcttgac gatcagcgcc gtatgatgac
    31981 tccgcagaaa ttgcgcgagt accaggacat catccgcgaa gtcaaagacg ccaacgcctg
    32041 atacacttaa ggcaaattaa ctcctcttcg aacccccgct tgtcgggggt ttttagcatt
    32101 gatggtgcga tatggaatca atcgaacaac aactgacaga actgcgaacg acgcttcgcc
    32161 atcatgaata tctttatcat gtgatggatg cgccggaaat tcccgacgct gaatacgaca
    32221 ggctgatgcg cgaactgcgc gagctggaaa ccaaacatcc agaactgatt acgcctgatt
    32281 cgcctaccca acgtgtaggc gctgcgccgc tggcggcttt cagccagata cgccatgaag
    32341 taccaatgct gtcactggat aacgtttttg atgaagaaag ctttcttgct ttcaacaaac
    32401 gtgtgcagga ccgtctgaaa aacaacgaga aagtcacctg gtgctgtgag ctgaagctgg
    32461 atggccttgc cgtcagtatt ctgtatgaaa atggcgtttt agtcagtgcc gcgacccgtg
    32521 gcgatggcac caccggggaa gatatcacgt ctaatgtgcg tactattcgc gctattccgc
    32581 tgaagctgca cggagagaat atcccggcgc gtctggaagt gcgtggtgaa gtgttcctgc
    32641 cgcaggcggg gttcgaaaag attaacgaag atgcgcgacg cacgggcggg aaagtttttg
    32701 ctaacccacg taatgcggca gctggttcac tgcgtcagct tgatccgcgt attacagcga
    32761 agcgaccgct cacttttttc tgctatggcg ttggtgttct ggaaggtggc gagctgccgg
    32821 atactcatct tggccgttta ctgcaattta aaaagtgggg gttgccggtc agcgatcggg
    32881 taacgctttg tgaatcggcg gaagaagtgc tggcgttcta tcacaaagtg gaagaagacc
    32941 gcccgacgct gggctttgat atcgacggcg tggtgattaa ggtcaactca ctggaacagc
    33001 aggagcagct tggctttgtc gcgcgtgccc cgcgctgggc ggtagcgttt aaattcccgg
    33061 cgcaggagca gatgaccttt gtgcgtgacg tcgagtttca ggttgggcgt actggcgcga
    33121 ttacgcctgt tgcgcgtctg gaacctgtcc atgttgctgg cgtgctggtg agtaacgcaa
    33181 ccttacacaa tgcggatgaa atcgaacgtc ttggtttacg cattggcgat aaagtggtga
    33241 ttcgccgcgc tggcgacgtg atcccgcagg tggttaacgt cgtgctttct gaacgcccgg
    33301 aagatacccg tgaggttgta ttcccgacgc attgtccggt atgtggttct gacgttgagc
    33361 gtgtggaagg tgaagcggtt gcccgctgta ccggtggcct gatttgcggt gcgcagcgta
    33421 aagagtcgct gaaacacttt gtttcccgcc gtgcgatgga tgttgacgga atgggcgaca
    33481 aaatcatcga tcagctggtt gaaaaagaat atgtccatac tccggcagat ctgttcaaac
    33541 tcaccgcagg caaactgacc ggactggagc gtatggggcc aaaatcggca caaaacgtgg
    33601 ttaacgcgct ggaaaaagcg aaagaaacca cctttgctcg cttcctctat gcacttggca
    33661 tccgtgaagt cggcgaggcc accgcagcag gtctggcggc atatttcggc acgctggaag
    33721 cgctggaagc cgcttcgatt gaagagctgc aaaaggtgcc tgatgttggc attgtcgttg
    33781 catcccacgt tcacaacttc tttgccgaag aaagcaaccg caatgtcatc agcgagctgt
    33841 tggcggaagg tgttcactgg cctgcgccga tcgttatcaa cgcggaagag attgacagcc
    33901 cgtttgctgg taaaaccgtg gtgcttacgg gcagcttaag ccagatgtcg cgtgatgacg
    33961 ctaaagctcg actggttgaa ctgggcgcga aagtcgcggg cagcgtgtcg aagaaaaccg
    34021 atctggtgat tgccggagaa gcggcaggtt ctaaactggc gaaagcgcag gaactgggca
    34081 ttgaagtcat cgacgaagcg gaaatgctgc gtttgctggg tagctgagat ggaaaaagag
    34141 cagctgattg aaatagccaa tacgataatg ccgtttggca aatataaagg gcgtcgctta
    34201 atcgacctgc cggaggagta tctgctgtgg tttgcccgca aagatgaatt cccggcaggg
    34261 aagctcggtg agctaatgca aatcacgctg ctgattaaaa ccgaggggct gacgcaactg
    34321 gtccagcccc tgaagcgtcc gctttaagct ttatcggcgc tgctttcctg ctgtgcctgt
    34381 aactgttcgg tctggcgttt gtatcgacgc gccagcaccg cacagaccat caattggatc
    34441 tgatggaaaa tcattagggg cagcaccatc ataccgatca ccgatgtggg gaacagaatg
    34501 tttgccatcg ggatgccatt tgccagactc tttttcgaac cacaaaagac gatagtaatt
    34561 tcatctgcct tattgaagcc cagtcggcgt gccataaaga cgttaactac aatcacgata
    34621 gccagaagaa cgcagctgac caccacgata aacagcaatg atccccagcc aactttatgc
    34681 cagataccat taacgacggc ttcgctgaat gctgtataaa ccaccaacag aatggacgtc
    34741 tggtcagttt tcgcaatcca ttttttattg cgcgacaccc agtcaccaat ccacggccgg
    34801 gaaagatgcc ccaacacaaa cggcagcagc agttgcagca taattttacc gacctgctca
    34861 aggctgcccc ctgcaccgtg aacattcatc accagaccaa ccagcaatgg tgaaaggaaa
    34921 atccccagca ggctggatgc tgacgcagaa caaaccgccg ccgcgacgtt accgcccgcc
    34981 attgacgtga aggcgattgc agactgcacg gtagccggga gaatgcacaa gtagagaaaa
    35041 ccggagtaga gcatcgggtc gacatttacc ggtttccacc aggcaaacag tacacccaga
    35101 atcggaaaca gcacgaaggt gctgcaaatt acccacaaat gcagtcgcca gtgaccaccg
    35161 ccagcaataa tcgcctcacg cgacaacttc gcgccgtgca taaagaacag cagggcaatc
    35221 gctgcggtgg tcagattttc aaagaagggg acgaaatcgc ctctggccgg aaagaaagag
    35281 gccagcaaca ccaccgtgat cagggttaag gtgaaaggat cgaggatacg aaaaagtttc
    35341 ataaagactc cagatgatcg atggttctat tgtgcttctt ccagtttgag aaataaaatt
    35401 gatttattgc atccattcat gaagaaaaca gatgaattat tctttaaaac aattaaaggt
    35461 tttcgtcaca gtagcgcagg agaaaagttt tagtcgtgca ggagagcgta tcggcctgag
    35521 ccagtcggca gtgagtcaca gtgtgaagga actggaaaat catactggtg ttcgcctgct
    35581 ggacagaacc acgcgtgaag tggtgcttac agatgcaggg cagcagctgg ctttgcgtct
    35641 tgagcgactg ctggatgaac tgaacagcac gttgcgcgat accgggcgta tggggcaaca
    35701 actgagcgga aaagttcggg tcgctgccag ccagaccatt tccgcgcatc ttattccgca
    35761 atgcattgct gaaagccatc gccgctatcc agatattcag tttgtcctgc acgatcgtcc
    35821 gcagcagtgg gtgatggaaa gtattcgcca gggagatgtc gactttggca tcgtcatcga
    35881 tccaggccca gtgggcgatc tgcaatgtga agcgattctt tccgagcctt tctttctgct
    35941 ttgccatcgc gatagcgctt tggccgtcga agattatgta ccctggcagg cattacaggg
    36001 agcgaaattg gtgttgcagg attacgcgtc aggcagccga ccgctgattg acgcagcgct
    36061 ggcgcgcaat ggtattcagg cgaatattgt gcaggagatt ggacatccgg cgacgctgtt
    36121 cccgatggta gccgcaggca tcggcatcag tattcttccc gcactggcgc taccattgcc
    36181 tgaaggtagc ccactggtgg taaagcgcat tacgccggtt gttgaacgcc agttgatgct
    36241 ggtgcgcagg aagaaccgtt cactttccac cgccgctgaa gcgttatggg acgtggtacg
    36301 agaccagggc aatgcgctaa tggctggtcg ggaaggggat ccgttgtatc agatatagag
    36361 tgaattcata ctgccgggag ccccggcagt aatcaatcag aagttaaatt tgatggagta
    36421 gattatcgca tcctggtaaa aatcttcgcc cagtttgtca tccgcatatc gatactgaat
    36481 ccccgtagta atgtgtgatg ttgcattcca ccacagtgca actgcgccat tcagcccttc
    36541 tttaccgcca ttacccgctg cataggtagc gtcacggtca aactcgtact cgttccagtt
    36601 ggtaagagtg aatttctcgc tgcccagcat aaagttgtaa cctgcaaccc agccggcgac
    36661 atagccgttg tcgccagtgt aataggtttg atctgtataa cgcttagcaa agaacggttt
    36721 gaaccaccaa ccgctgccgg tgaaattgta gccgataccg tacaggaaca tatcgtcgtg
    36781 gaaattcacg cgattcgcag aaccgtaggt gccatacgca tgcaagtaga gattaaatcc
    36841 ggtgtcaccc aggtaaatac ggttggtatt tttaaaggta taacgctgct cgctacctgg
    36901 tttattatgg cgaccgttat agaagttttc ccagtcgaag aacccgtaca tttctcccca
    36961 actaaagtta gcgccacctt caaattcaag ataaccaaaa tcatctttgt gtgatttggt
    37021 cgacgatttt tcggtagtcc ggctggtcca gtccagataa tgcacgccga tatctgcaaa
    37081 accgccttta aattctgcat gagatacaac aggtatcgct aatatagagg aaagtgtcag
    37141 agttaaaaga tgttttttca tagcataatt ccctatgccg atcgttatta gtagattgct
    37201 taataaagtg gtttgctaaa aacgacatat tgttaatgca aaggaattaa tatcgccaat
    37261 acagttttta ccgtggatta atagagaaaa gatataaatc catcgatatc gaatcgctat
    37321 tgaatgcgtg agaaaagtgt attggtaaga gccgggacaa cctcgctata aaaaggcgtt
    37381 agattccacc ctacagaaaa aggatatgta tgtctcaggt tcaattttct cataacccgc
    37441 tgttttgcat agatattatc aagacttata aacctgattt cacgccacga gtggccttta
    37501 ttttaggttc cgggctcggc gcgctggccg atcagattga gaacgctgtc gcaatttcct
    37561 acgaaaagct gccgggcttc ccggtaagta cggtacatgg tcatgcgggg gagttagtgc
    37621 tgggccatct ctagggcgta ccggtggtat gtatgaaagg tcgcggacat ttctacgaag
    37681 gtcgtggaat gaccattatg actgacgcaa ttcgtacctt taagctgctg ggctgcgagc
    37741 tactgttctg caccaatgcg gcaggctcgc tgcgtccgga agtgggggca ggcagcctgg
    37801 tcgcattgaa agatcatatc aacaccatgc ccggtacgcc gatggtgggt cttaacgatg
    37861 atcgttttgg agagcgcttc ttctcactgg caaatgccta cgatgcggaa taccgcgcac
    37921 tgttacaaaa agtggcgaaa gaagagggtt tccctctgac ggagggcgtg ttcgtctcgt
    37981 atccggggcc gaatttcgag actgcggcgg aaattcgcat gatgcaaatt attggtgggg
    38041 atgttgttgg tatgtctgtg gtgcctgagg ttatttcagc tcgccattgc gaccttaaag
    38101 tcgttgcggt ctctgcgatt accaatatgg cggaaggttt aagcgatgtg aaattgtcac
    38161 atgcgcaaac gctggcggct gcggagctct ctaagcagaa cttcatcaac cttatttgtg
    38221 gctttctgcg caaaattgcc tgagaaaaac aataagaaac ggcctcgcgg gtgaggccaa
    38281 atgatgcgac aaggaaacga ttatgagcat cgcgatgcgc ttaaaggtaa tgtccttttt
    38341 gcaatatttt atctggggga gctggctggt taccctcggc tcttacatga ttaataccct
    38401 tcatttcacc ggcgctaatg ttggcatggt ttacagttcc aaagggatcg ccgcgattat
    38461 tatgcctggt ataatgggga tcatcgcaga caaatggctg cgcgcagaac gtgcatacat
    38521 gctgtgtcac ctggtgtgtg cgggcgtact tttttatgcg gcatccgtaa ctgatccgga
    38581 tatgatgttt tgggtgatgt tagtcaatgc gatggcgttt atgccgacta ttgcgttatc
    38641 gaacagcgtc tcttattcct gtcttgccca ggcagggctt gacccggtga ccgctttccc
    38701 gcccattcgc gtttttggta cggtggggtt cattgtcgcg atgtgggcag taagcctgct
    38761 gcatctggaa ttgagtagtc tgcagctgta tatcgcgtcc ggtgcgtcat tgctgctgtc
    38821 ggcttatgcg ctgactttgc cgaagattcc ggttgcggag aaaaaagcga ccacatcgct
    38881 tgccagcaag ctgggtctgg atgccttcgt gctgtttaaa aatccacgca tggccatctt
    38941 tttcctcttt gccatgatgc tgggtgcggt actgcaaatt accaacgttt ttggtaatcc
    39001 gttcctacat gatttcgccc gtaacccgga gtttgctgac agttttgtgg tgaaatatcc
    39061 ctccatttta ctgtcagttt cacagatggc agaagtgggc tttatactga ctatcccatt
    39121 ctttttaaag cgatttggca ttaaaaccgt catgctgatg agtatggtgg cctggacgct
    39181 gcgctttggc ttcttcgcct atggcgatcc gtcaacaacc ggatttattt tgctgctgct
    39241 gtcgatgatt gtttatggct gtgcattcga tttcttcaat atttctggtt cggtatttgt
    39301 cgaacaggaa gttgattcca gcattcgtgc cagcgcgcag gggctcttta tgaccatggt
    39361 aaatggtgtc ggcgcatggg ttggctcgat tctgagtggc atggcagtag attacttttc
    39421 ggtggatggc gtaaaagact ggcaaactat ctggctggtg tttgcaggat atgctctttt
    39481 tctcgcagtg atatttttct ttgggtttaa atataatcat gaccctgaaa agataaagca
    39541 tcgagcggtg gctcattaaa aggattcgca gctctgctct tcagagctgc ttttatgata
    39601 aaggttaatt agtgaaagat attttatttt cttccggcgt tggttttggc attggtgctt
    39661 tgtttacaat cgtgcgcttg cctattcctg taccaaatgt attaccaggt atattatcca
    39721 ttgtgtttat gtatgtcgga tatctggtgg tgaaatactt tatgccatga taatttaata
    39781 cgatgtattt attatatgga gcacttaatt atggaacgcg tatacagaac agatcttaag
    39841 ttgctccgtt attttcttgc cgtagcggaa gagttgcatt ttggccgcgc agcagcgcgt
    39901 ttaaatatgt ctcagcctcc gctcagtatt catattaaag agctggaaaa tcaactcggc
    39961 acgcagcttt ttattcgcca ttcgcgcagc gtcgtactga cacacgcggg caaaatcttg
    40021 atggaagaat cgcgtcgatt gctggtgaat gctaataatg tattggctcg ggttgaacaa
    40081 ataggtcggg gagaagcagg gcggattgaa ctcggcgtag tgggaacggc tatgtgggga
    40141 cggatgcgcc cggttatgcg gcgattcctc agggaaaatc ctaacgttga agttcttttt
    40201 cgcgaaaaga tgcccgcgat gcaaatggcc ctgctggaac gacgcgaact cgatgccggg
    40261 atctggcgaa tggcgacaga accaccgaca ggttttacca gcttacggtt gcatgaatcg
    40321 gcgtttctgg tggcgatgcc tgaagagcat catctctcat cattttccac cgttccgctg
    40381 gaagcgctac gtgacgagta ttttgttaca atgccgcccg tgtacactga ctgggatttt
    40441 ttgcagcgag tttgccagca ggtgggattt tcaccggttg ttattcgcga agttaatgaa
    40501 ccgcaaacgg tactcgccat ggtcagtatg ggtattggta tcacattgat agcggacagc
    40561 tacgcacaaa tgaactggcc aggtgtcatt ttccgtccgc tcaaacagcg catccctgca
    40621 gatttatata ttgtttatga aacacagcag gtgacgcccg cgatggttaa gctgttggct
    40681 gcattgacac agtaggtgag gagacaggaa ttgccggaaa tagtcgtctg ctaagtgaag
    40741 cgcatcaggc aatttcgcaa atcgcagata gcaaaaaagc gcctttaggg cgctttttta
    40801 cattggtggg tcgtgcagga tgactcgctt cgctcgccct tcgggccgtc gccgcgagcg
    40861 gctccgttgt ttcgctgcgc tcaactcgaa cctgcggcag cttcgaatct ttgagtatcg
    40921 cagatagcaa aaaagcgcct ttagggcgct ttttttacat tggtgggtcg tgcaggattc
    40981 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgaag
    41041 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    41101 gctgagctaa tcacccactt cggtacttca catttttaca agaaatttag ctggcgagaa
    41161 agtggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc
    41221 cagctgagct aatcacccac ttctcaattt cttgctacac ggcggagact acataaagta
    41281 gttggtgggt gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc
    41341 cagctgagct aatcaccccc gctgtgtgga gtcgcattat agggagagtt caaaatgagt
    41401 caacgcattt tctaaagaaa ttgttcgttc gtcgtaaatt taagcaagat gatcgcaaaa
    41461 cagaccgtgt tgcgcaattt gtcaacgaaa acaataatgc gtaaggttgc aacccgaact
    41521 acattgagga atcaggcggg agtgatagaa tatcgcccac tcaatttttc caggatttgc
    41581 cggttgtcgg catctttcta aacgtaaggc catttcatga aaatcaaaac tcgcttcgcg
    41641 ccaagcccaa caggctatct gcacgttggc ggcgcgcgta ctgctcttta ctcctggctt
    41701 tttgcacgta accacggcgg tgagttcgtg ctgcgtattg aagacaccga tcttgagcgt
    41761 tccacgccgg aagctatcga agccattatg gatggcatga actggctgag cctggagtgg
    41821 gatgaaggtc cgtactacca gaccaaacgt tttgatcgct acaatgcggt gatcgatcag
    41881 atgctggaag agggcactgc ttataaatgc tattgctcta aagagcgcct ggaagcgctg
    41941 cgcgaagagc aaatggcgaa aggtgagaag ccgcgttatg acggtcgctg ccgccacagc
    42001 catgagcatc atgctgatga tgaaccgtgt gtcgtgcgtt ttgctaatcc gcaggaaggt
    42061 tctgttgttt ttgacgatca gatccgtggt ccgatcgagt tcagcaacca ggagctggat
    42121 gatctgatta tccgccgtac cgatggttcc ccaacctata acttctgtgt ggttgtcgat
    42181 gactgggata tggaaatcac ccacgttatc cgtggcgaag accatatcaa caacacgccg
    42241 cgccagatca acattcttaa ggccctgaaa gcgccggtgc cggtttacgc gcacgtttct
    42301 atgatcaatg gcgatgacgg taaaaaactg tccaaacgtc acggggcagt cagcgtaatg
    42361 cagtatcgtg atgacggtta tttgccagaa gcactgctga actatctggt gcgtctgggc
    42421 tggtcccacg gcgatcagga aatcttcact cgtgaagaga tgatcaaata cttcactttg
    42481 aatgccgtca gcaaatctgc cagtgcgttc aacaccgaca agctgctgtg gctgaaccat
    42541 cactacatta acgcgctgcc gccggagtat gttgctactc acttacagtg gcacattgag
    42601 caggaaaata tcgatacccg taacggcccg cagctggctg atctggtgaa actgctgggc
    42661 gaacgctgca agacgctgaa agagatggca cagagctgcc gttatttcta cgaagatttt
    42721 gctgagttcg atgccgatgc ggcgaaaaaa catctgcgtc cggtagcgcg tcagccgctg
    42781 gaagtggttc gtgacaaact ggccgcgatt actgactgga ccgctgaaaa cgttcatcac
    42841 gctattcagg cgacggcgga tgagctggaa gtgggtatgg gtaaagttgg tatgccgctg
    42901 cgtgtcgccg taaccggtgc ggggcagtct ccagcactgg atgttaccgt tcacgcaatt
    42961 ggtaagaccc gcagtatcga gcgtatcaac aaagcgctgg attttattgc tgaacgcgaa
    43021 aatcagcagt aattagcgtg taaaaagata aacggcagga gataatatcc tgccgttttt
    43081 tatttatgct gattcgtcaa tatcgagacg ggcgaggaaa ttgcgaattc cttcacgtga
    43141 caaaaattgc gcgacttttt cctgctcaat gtctgtcata ttatctatcg cgttgatgat
    43201 ttgtcgatac gccggggcgc gtgctccagg agcgtattca gcaattctct cttcagcttc
    43261 catcaacatt ggtgtgttat ggaaggctgg cgtgttctga ataaactcgc aaacttgcgt
    43321 atcgaccaga ataagtctgg cacgaccgcc tttcacgccg ggaaattttt ccgttttcca
    43381 gcctttttct ctgatccaac ggttaacagt ttgtttagcc acaccgaggc attcagccag
    43441 ttcttcggtg gtcattttgt tgcgtaatct tttcatatta tttgctatcg cgaatcccta
    43501 agcgttgtaa caatccgata atcccttccc gcagaagcaa ggatgtaaac tgtttttgtt
    43561 caactggcgt catctctttc gccagtgtca ccagcaatac ttcaagcggc gcatcaccag
    43621 aaagggcagg cgcttctccc tgaccttctg gacgttcagc attgcgaata tattcacgaa
    43681 cctgttcatt gacgtgaacc agtcgggctt tgccaccctg gacgccaggt tttggtgacg
    43741 ttgtccagcc ttccttgcgt acccatttat taatggtctg gcggctatag ccggtcagtc
    43801 tggcaagttc atctggtgtc atcctctcct tgaacataac aattccctga atgtatgtgg
    43861 gtctattagt agttcatttt ataacaccac tttacaggaa accgtgacat gaataactac
    43921 tgaatgcgag cggttttcca aaattgttca tttgcctgat ttttcagcga ttgaaactac
    43981 acgttgcaat ttgccgttga cacattcggg cggaattcat atgatgccgc ccgtcaactc
    44041 gacaagcttt acgtgacggg gctatagctc agctgggaga gcgcttgcat ggcatgcaag
    44101 aggtcagcgg ttcgatcccg cttagctcca ccaaattttg cacccagcaa atttggtacg
    44161 taaacgcatc gtggggctat agctcagctg ggagagcgct tgcatggcat gcaagaggtc
    44221 agcggttcga tcccgcttag ctccaccaaa tttccaaccc tcgctgcaaa gcgggggttt
    44281 tttgtctctg ctttttgccg cttttgtaat acagtctacg tccgggttag cgccgcctgg
    44341 tgaaagcatc attggatgaa aaatcggcaa caggctggcc cctgtttgct tcgcgatgcg
    44401 aataaactta ttatttgtgt gcctgaaaac cccgatcagt gagagtagtg tactcatgtt
    44461 tgtggagcat aacctgataa aaaatatcaa gatattcaca ctagcgttta cgctcaccgt
    44521 ggtacttatt cagctatccc gttttatttc gccacttgcc attatccatt ccagttatat
    44581 ctttctggcg tggatgccac tgtgcgtaat gctgtcaatc ttgtttatct ttggctggcg
    44641 cggtgtcgtt cccgttttat gcgggatgtt ttgcaccaat ctgtggaact ttcatctctc
    44701 ttttttacag actgcggtca tgctcggtag ccagacgttt gtcgtgttgt gtgcctgcgc
    44761 aatattacgc tggcagctgg ggacgcgttg gcgttatgga ttgaccagcc gatatgtctg
    44821 gcaacgtctg ttctggcttg gtttggtgac gccgatcggc atcaaatgca gcatgtatct
    44881 tgtggggagc ttctttgatt tcccgctaaa gatatccacc tttttcggcg atgcggatgc
    44941 cattttcacg gtcgttgatt tgctaagcct tttcaccgct gtgctgattt acaacatgct
    45001 tttctactat ctcactcgca tgattgtaag tccccacttt gcgcagatat tgtggcgcag
    45061 ggatatcgct ccgtcgttgg gcaaagagaa acgcgcattt accttaagct ggctggcagc
    45121 gttaagcgtg ctgctacttc tgttgtgcac accttatgaa aacgacttta ttgccggtta
    45181 cttggtaccc gttttcttca tcatctttac cctcggggtc ggtaagcttc gctatccgtt
    45241 tttaaatctc acctgggctg tttcaacgct ttgcctgctg aattacaacc agaacttttt
    45301 gcaaggggtg gaaaccgaat attcgctggc atttattctt gcggtgctga tttcctttag
    45361 cgtttgcctg ctctatatgg tgcgcattta tcatcgcagt gaatggctta atcgccgctg
    45421 gcatttgcag gcgctgacag atccgttaac gctcctaccc aactttcgtg cgttggaaca
    45481 agcgccggag caagaggcgg gcaagagttt ttgctgcctg cgcattgata atcttgagtt
    45541 tatgagtcgt cattacggct taatgatgcg cgttcactgt atccgctcaa tttgccgtac
    45601 gctgctgccg ttgatgcagg aaaacgaaaa gttgtatcaa ttgccgggta gtgaactgct
    45661 gttagtgctg agcgggccgg aaacggaagg gcgactccag catatggtta acatcctgaa
    45721 tagtcggcaa attcactgga acaataccgg gctggatatg ggctatggtg ctgcctgggg
    45781 gcgttttgat ggaaatcagg aaaccctgca acccttgttg gggcagttaa gctggctggc
    45841 ggagcaatcc tgcgcacatc atcatgtgct ggcgctggat agcagagagg agatggtttc
    45901 cgggcagacc actaaacagg tgctattgct gaataccatt cgcacggcgt tagatcaggg
    45961 tgatttgctg ctctacgccc agccaattcg caacaaagag ggtgacggtt atgatgagat
    46021 cctcgcgcga ctgaaatatg acggcggcat tatgaccccg gataagtttc tgccccttat
    46081 tgctcagttt aaccttagcg cgcgttttga tttgcaagtg ctggaatcct tgttgaagtg
    46141 gctggcaaca cacccttgcg acaaaaaagg accgcgcttt tcagtcaatt taatgccgct
    46201 cacgctgctg caaaagaata ttgccgggcg gattattcgt ctgtttaagc gttatcacat
    46261 ctccccgcag gcggtcattc ttgagatcac cgaggagcag gcgttttcta acgcagaaag
    46321 cagcatgtac aacatcgagc agctgcataa gtttggtttc cggattgcga ttgatgactt
    46381 tggcaccgga tatgccaact acgaacggtt aaagcgtttg caggctgata tcatcaaaat
    46441 tgatggcgtc tttgtgaaag atattgtcac gaatacgctg gatgcgatga ttgtgagatc
    46501 aattaccgat ctggcgaaag cgaagtcatt gagtgtggtc gcggagtttg tcgagacgca
    46561 acagcagcag gcgctattgc ataagctcgg ggtgcaatat ctgcaagggt atttgattgg
    46621 tcgcccgcag ccattagctg attaacaggc gtaaaaaaac cggggaatta tccccggctt
    46681 ttttatgtat ggtcttacag caccagtgct gcgattgacg cagacagcac actcaccagg
    46741 gtagagccgt aaaccagctt cagaccgaag cgagaaacca cgttaccttg ctcttcattc
    46801 aggcctttaa ctgcacctgc gataatcccg attgaagaga agttagcgaa ggaaaccagg
    46861 aacacagaga tgatgccttc agcacgcgga gagagcgtgg aagcaatttt ctgcagatcc
    46921 atcatcgcaa cgaactcgtt ggaaaccagt ttggtcgcca tgatactgcc cacttgcagt
    46981 gcttcactgg aaggaacacc catcacccat gcaatcggat agaagatgta gcccaggatg
    47041 ccctggaagg agatgctgta gccaaaccag ccagtaacgg tggcaaacag tgcgttcagc
    47101 gcggcgatca gggcgataaa gccgatcagc atcgcggcaa cgataatggc aactttgaaa
    47161 cctgccagaa tgtattcacc cagcatttcg aagaagctct gaccttcgtg caggttggac
    47221 atctggatgt tttcttcact ggcatcaaca cggtaaggat tgatcagcga cagcacgata
    47281 aaggtgctga acatgttcag taccagcgca gcaacgacgt atttcggttc cagcatggtc
    47341 atgtatgcac caacgatgga catcgacacg gtggacattg ccgtggcagc catggtgtac
    47401 atacgattac gggagatttt gccgaggata tctttatagg caataaagtt ttcagactga
    47461 cccagaatca gggagctgac ggcgttaaag gattccagtt tgcccatgcc gttgactttg
    47521 gagagcagga aaccaattgc gcggatgatc accggcaaca cgcgaatgtg ctggagaata
    47581 ccgatcagtg cagagataaa gacgattggg cacagcactt tcaggaagaa gaatgccagg
    47641 ccttgatcat tcatgctacc aaagacgaag ttagtccctt cgttggcaaa tccgagcagt
    47701 ttttcgaaca tttcggagaa gcctttcacg aagcctaaac caacgtcgga gttcaggaag
    47761 aaccacgcca gtaacacttc gataacaagc agttgaataa cataacggat acgaattttt
    47821 ttgcggtcgc tgcttaccag cagtgcgaga atcgcaacaa cggcaagtgc cagtacaaaa
    47881 tgaaggacgc ggtccatatt tgctccaaat atgaggcagg ttaaatttcc gtgcacattc
    47941 tatgtaacat ggttaaggaa aacgagatct gacacacact ataatgacgt cctgtgacaa
    48001 gcttcaaaaa tagtgatctg tcatacattt ctgctatgtt gtgtatggaa gctgaaagtt
    48061 atgtaaatgt gctaacatta aacataacca taacattcaa gtcatcaatc ttttcgtagt
    48121 cataatcctg gtctatcaaa gaaatcacca caacccattc aaatgaattt gataatcatt
    48181 ctcgtttggc atagcatgaa acatagcaaa ggctatgttt tagaggcaca agatgacgaa
    48241 ctatcgcgtt gagagtagca gcggacgggc ggcgcgcaag atgaggctcg cattaatggg
    48301 acctgcgttc attgcggcga ttggttatat cgatcccggt aactttgcga ccaatattca
    48361 ggcgggtgct agcttcggct atcagctact gtgggttgtc gtttgggcca acctgatggc
    48421 gatgctgatt cagatcctct ctgccaaact agggattgcc accggtaaaa atctggcgga
    48481 gcagattcgt gatcactatc cgcgtcccgt agtgtggttc tattgggttc aggcagaaat
    48541 tattgcgatg gcaaccgacc tggcggaatt tattggtgcg gcgatcggtt ttaaactcat
    48601 tcttggtgtt tcgttgttgc agggcgcggt gctgacgggg atcgcgactt tcctgatttt
    48661 aatgctgcaa cgtcgcgggc aaaaaccgct ggagaaagtg attggcgggt tactgttgtt
    48721 tgttgccgcg gcttacattg tcgagttgat tttctcccag cctaacctgg cgcagctggg
    48781 taaaggaatg gtgatcccga gtttacctac ttcggaggcg gtcttcctgg cagcaggcgt
    48841 gttaggggcg acgattatgc cgcatgtgat ttatttgcac tcttcgctca ctcagcattt
    48901 acatggcggt tcgcgtcaac aacgttattc cgccaccaaa tgggatgtgg ctatcgccat
    48961 gacgattgcc ggttttgtca atctggcgat gatggctaca gctgcggcgg cgttccactt
    49021 ttctggtcat actggtgttg ccgatcttga tgaggcttat ctgacgctgc aaccgttgtt
    49081 aagccatgct gcggcaacgg tctttggatt aagcctggtt gctgccggac tgtcctcaac
    49141 ggtggtgggg acactggcag ggcaggtggt gatgcagggg ttcattcgct tccatatccc
    49201 gctgtgggtg cgtcgtacag tcaccatgct gccgtcattt attgtcattc tgatgggatt
    49261 agatccgaca cggattctgg ttatgagtca ggtgctgtta agttttggta tcgccctggc
    49321 gctggttcca ctgctgattt tcaccagtga cagcaagttg atgggcgatc tggtgaacag
    49381 caaacgcgta aaacagacag gctgggtgat tgtagtgctg gtcgtggcgc taaatatctg
    49441 gttgttggtg gggacggcgc tgggattgta gttgaatgag cgtcgcatct ggcactattg
    49501 gcggatgcgg cgtaaacgcc ttatccgccc tacgcgttaa agagccggtt tgtaggcctg
    49561 ataagacgcg ctagcgtcgc atcaggcatt gtgctccatt ggaggacgcg tttaatggcg
    49621 gtgcccatgt ccgcggcctt ttccgcgatg atcgtcacga tcgcgccagc cttcacgata
    49681 gccacgctca tatgctttac gcttatccca gccacgatga tagccattat catgacgcca
    49741 ccagcggttt ttgcgccatt cataattacg atgccagtaa tcacgatctc gccagtgacc
    49801 accgtcccag taattaccgt aatgatcgcg atcgccaatt tgtaatttga tcgatggcag
    49861 tagggtgatt tcacctgcat ttgctgcaag cggggtaaat gccatcaggg cggccgccag
    49921 aaacagtgac ctgaacattg ttattctcct tcacgatcga agccgtcagc ggcctgttaa
    49981 cgcaatatta cggggaggta aagccccgta tcatcgccat aactcttaaa tcataaagaa
    50041 gagcattttt tgctaaacat tatcgtgggg tggctgttgc ttcggacgat tgaccatcat
    50101 catcggctta cctgctaata acagccatgc cggaagcatg acgatacaga gcagcggtag
    50161 caatgtggta ttgggtacaa cgactgccgc cataaaaaga cttaaccagc catcgcgtgt
    50221 taccaccagc acgatgccga gaatagcgca ggaaacggtt attgccgctg gaacggcggg
    50281 aacgtgctca tgcagcatca accctaatgc cacgccgaca aacactgccg ggaagattcg
    50341 cccaccgcga aagccactgg cggcagcaac gaccagggcg gcaagtttaa ttaccgccag
    50401 caaaaagtaa tcgctggtgc tgaaagcctg atttgccacc atctgctgca tctcatccag
    50461 ccctttaaac agcgaaaccg gcccaccaat aactcccaga ataccgagaa taaatccgcc
    50521 aatacctagc acgagcaccg gatttttcat ttgatgcatc atcgcgtgca accgtggtaa
    50581 gcaccatacg gcgaccattc ctgctgcgat ggcaatcgcc gcgacaattg caccgctgag
    50641 aatatcggtc atttccatct gtccgtaatg cgcaatgggc agtgaaaaat ggggatggaa
    50701 aaataatccg gtagtaagtg caccagctgc tgccgccatt aatggcgcaa aaagacgatc
    50761 ccatagcgga acttcactac tgccatttaa ggtttgcgaa aatatcaacg ccgccgcaac
    50821 aggtgtacca aacagcgcgc cgatggttcc ggcagaggct aaaatagtcc actccattcg
    50881 gttgacgcgc ggtaacagac gagcgccaat ggcaaccgca agggcgatat tgacggtcat
    50941 gatcggatgt tccggcccca ggctgacgcc gccagcaaga ccgagaatta atgctacgat
    51001 aagtcctggt agcgcagagg gcggaactgg tgcgccgatc agcggttcac aggcggggtc
    51061 tggtccggca tgaccctggc tgaaacggat aaccaacccc accgcaatac ccgttagcgt
    51121 taatacaccg atgatccaga gaggtgaatc ctgggctatc cccagagttc ccggcagtcg
    51181 ttgccagagc aaattctgta ataccgaggc gattttcatc accacaatca gaataagact
    51241 ggacgcaatc ccaattgcca cggcggggag cgataataac aacatggttc tggctcgcgg
    51301 atggagcatg ttctattcct tatgcggggt cagatactta gtttgcccag cttgcaaaaa
    51361 ggcatcgctg caattggtgc tgaaacgata aagtaaatgt gtgacccaga tcgatattta
    51421 cagggagcct gcctttccgg cgttgttgtt atgcccccag gtatttacag tgtgagaaag
    51481 aattattttg actttagcgg agcagttgaa gaatgacaaa gtatgcatta gtcggtgatg
    51541 tgggcggcac caacgcacgt cttgctctgt gtgatattgc cagtggtgaa atctcgcagg
    51601 ctaagaccta ttcagggctt gattacccca gcctcgaagc ggtcattcgc gtttatcttg
    51661 aagaacataa ggtcgaggtg aaagacggct gtattgccat cgcttgccca attaccggtg
    51721 actgggtggc aatgaccaac catacctggg cgttctcaat tgccgaaatg aaaaagaatc
    51781 tcggttttag ccatctggaa attattaacg attttaccgc tgtatcgatg gcgatcccga
    51841 tgctgaaaaa agagcatctg attcagtttg gtggcgcaga accggtcgaa ggtaaaccta
    51901 ttgcggttta cggtgccgga acagggcttg gggttgcgca tctggtccat gtcgataagc
    51961 gttgggtaag cttgccgggc gaaggcggtc acgttgattt tgcgccgaat agtgaagaag
    52021 aggccattat cctcgaaata ctgcgtgcgg aaattggcca tgtttcggcg gaacgcgtgc
    52081 tttctggccc tgggctggtg aatttgtatc gcgcaattgt gaaagctgac aaccgcctgc
    52141 cagaaaatct caagccaaaa gatattaccg aacgtgcgct ggctgacagc tgcaccgatt
    52201 gccgccgcgc gttgtcgctg ttttgcgtca ttatgggccg ttttggcggc aatctggcgc
    52261 tcaatctcgg gacatttggc ggcgtgttta ttgcgggcgg tatcgtgccg cgcttccttg
    52321 agttcttcaa agcctctggt ttccgtgccg catttgaaga taaagggcgc tttaaagaat
    52381 atgtctatga tattccggtg tatctcatcg tccatgacaa tccgggtctt ctcggttccg
    52441 gtgcacattt acgccagacc ttaggtcaca ttctgtaaat ccttcctgtt atatcgggag
    52501 gtaactctcc cgataatctt ttaaatcata cagttaattc aatttttctt tgtgtcccct
    52561 cacaagaccg acctgcgtca cacttccgta cagcgggatt aattctccag taaatgcatt
    52621 atttgtctgg taacggcgat ttgttttgca cgttcataat ttcactcgtc aactatcact
    52681 gaatgaggaa ttaacgatga gtaagaaact aattgcctta tgtgcctgcc caatgggcct
    52741 ggctcacact tttatggccg ctcaggcgct ggaagaagcg gcggtagaag ccggttatga
    52801 agtgaaaatt gaaacccagg gcgcggacgg tatccagaat cgcctgacgg cgcaggatat
    52861 cgccgaagca accatcatca tccactccgt ggcagttacc ccggaagata atgagcgttt
    52921 cgaatcacgc gacgtttatg aaatcacttt gcaggacgca attaaaaacg ctgcgggcat
    52981 catcaaagaa atcgaagaga tgattgcctc tgaacaacag taatattcga cagggataat
    53041 tgttatggcc attaaaaaac gcagtgcaac cgttgtgcct ggcgcatccg gtgcggcagc
    53101 ggcagttaag aatccgcagg cctctaaaag cagcttctgg ggtgaactcc cgcagcatgt
    53161 gatgtcaggg atttcacgca tggtgccgac cttaattatg ggcggtgtga tcctcgcttt
    53221 cagccagctg attgcttata gctggcttaa aattcctgct gagatcggca tcatggatgc
    53281 tcttaatagc gggaaattct ccggtttcga cctctcctta ctgaaatttg cctggctgtc
    53341 gcagtccttc ggcggcgtgt tgtttggttt tgccattccg atgtttgctg cttttgtggc
    53401 gaactctatc ggtggcaaac tggcgttccc ggctggtttt atcggcggct tgatgtctac
    53461 ccagccgacg caactgctga acttcgatcc cagcactatg caatgggcga cctcttcgcc
    53521 ggtgccgtcc accttcattg gcgcgctgat tatttctatc gttgccggtt accttgtgaa
    53581 atggatgaac cagaaaatcc agttaccgga tttcctgtta gcgttcaaaa ccacattttt
    53641 gctaccgatt ctttccgcca tttttgtcat gctggcgatg tactacgtca tcaccccgtt
    53701 tggcggctgg atcaacggcg gtatccgtac tgtgctgact gccgcaggtg agaagggcgc
    53761 gctgatgtac gcaatgggga tcgccgcggc gacagcaatc gaccttggtg gcccgatcaa
    53821 caaagccgca ggtttcgttg ccttcagctt taccactgac cacgtattac cggtcaccgc
    53881 tcgttctatc gctatcgtta ttcctccgat tggtctgggt ctggcgacca ttattgaccg
    53941 tcgtttaacc ggcaaaggcc tgttcaacgc tcaactttat ccgcagggta agaccgcgat
    54001 gttccttgcc tttatgggga ttagtgaggg tgcgattccg tttgcgctgg aaagccccat
    54061 caccgccatt ccgtcgtata tggtcggcgc gattgtcggc tcaaccgccg ctgtctggct
    54121 gggcgcagtg caatggttcc cggaatccgc tatctgggca tggccgctgg tcactaacct
    54181 gggcgtctat atggcgggga tcgcgctggg ggcagtcata actgcgctga tggtggtgtt
    54241 cctgcgtctg atgatgttcc gtaaaggcaa attgttaatc gatagcctgt aagaaggaaa
    54301 atccagatga cattactcgc ttcgctgcgc gactggctta aggcgcaaca actggatgca
    54361 gtgcttctct cctcacggca gaacaaacag ccgcatctgg ggatctccac cggatcaggc
    54421 tatgtggtta ttagccgtga aagtgcgcac attctggtag attcgcgcta tttcgttgag
    54481 gtggaagccc gtgcgcaagg ctaccagctg catttgcttg acgcgacgaa cacgcttacc
    54541 actatcgtca atcaaatcat tgccgatgag cagttgcaaa cgctcggttt tgaaggccag
    54601 caggtgagtt gggaaaccgc gcaccgctgg aagtctgaac tcaatgcgaa actggttagc
    54661 gccacgccgg atgtgctgcg gcaaatcaaa acgccagagg aggtggagat aatccgcctc
    54721 gcctgtggga ttgccgatcg cggagcggag catattcgcc gctttattca ggcggggatg
    54781 agcgagcgcg agatagccgc tgaactggag tggtttatgc gccagcaggg cgcagaaaaa
    54841 gcctcttttg acaccattgt cgccagcggc tggcgcgggg cgctgccgca cggcaaagcc
    54901 agcgacaaga ttgttgcagc gggcgagttt gtcactctcg attttggtgc gctgtatcag
    54961 ggctactgct ctgatatgac gcgcaccttg ctggtgaatg gcgaaggggt gagcgccgaa
    55021 tctcacccgc tgtttaacgt ctaccagatt gtcctgcagg cacagctcgc agcaatctct
    55081 gcaattcgcc ccggcgtgcg ctgccagcag gttgacgatg ccgcgcgcca agtgattacc
    55141 gaggcaggtt ttagccacta tttcggtcat aacaccggtc atgctatcgg cattgaagtt
    55201 catgaagatc cgcgtttttc accgcgggac accacgacgc tacagccagg catgttactg
    55261 accgtggagc cggggattta tttgccaggg caagggggcg tgcgcatcga agatgttgtg
    55321 ctggttaccc cgcaaggcgc agaagtgctc tacgccatgc cgaaaacagt gttgctcacg
    55381 ggagaggcat aatggattta tcgctattaa aagcgttgag cgaggcagat gcgatcgcct
    55441 cctcggaaca ggaagtgcgg cagatcctgc tggaagaagc ggatcgcctg caaaaagaag
    55501 tgcgatttga tggtctggga tcggtgctga tccgcctcaa tgaatcgaca ggtccgaagg
    55561 tgatgatctg cgcccatatg gacgaagtgg gatttatggt gcgtagcatc tcccgcgaag
    55621 gggcaattga tgtgctgccg gttggcaacg tgcgcatggc tgcccgccag ctgcagccgg
    55681 tgcgcatcac cacccgtgaa gagtgcaaaa ttccaggcct gcttgacggc gaccggcagg
    55741 ggaatgacgt cagcgccatg cgcgtggaca ttggtgcgcg ctcgtatgac gaagtgatgc
    55801 aggcgggaat tcgtccaggc gatcgcgtca cctttgatac aacttttcag gttctccctc
    55861 accagcgggt gatggggaaa gcctttgatg atcgcctcgg ttgctatctg ctggtgacgt
    55921 tactacgtga gctgcacgat gccgaactgc ctgcggaggt gtggctggtg gcaagttcca
    55981 gcgaagaggt gggattacgc ggtgggcaaa ccgctacccg cgcggtgtcg ccggacgtcg
    56041 ccattgtgct ggataccgcc tgctgggcga aaaactttga ttatggcgcg gctaaccatc
    56101 gccagattgg taacggcccg atgctggtgt taagcgacaa gtcactgatt gcgccgccaa
    56161 aacttaccgc ctggatcgaa accgtggcgg ctgaaattgg cgtaccgttg caggcagata
    56221 tgttcagcaa cggcggcacg gacggcggag cggtgcactt aaccggtact ggcgtaccca
    56281 cagtggtaat ggggcctgcc acccgccacg gacattgcgc ggcatcgatt gccgattgcc
    56341 gcgacatttt gcagatggag caacttttat ctgcccttat tcaacgtctt acgcgtgaga
    56401 cggttgttca actgacggat ttcagatgat ctcctgatta acccggagcg gttatgttaa
    56461 cgattcaatt tctctgtcct ttgcctaacg gtctacatgc tcgtccggcg tgggaactta
    56521 aagaacagtg cagccagtgg caaagcgaaa tcaccttttt caaccatcgc cagaacgcaa
    56581 aggccgatgc taaaagctcg ctggcgttga ttggaactgg caccttattt aatgacagtt
    56641 gcagcctgaa cattagcggc agcgatgaag agcaggcgcg gcgcgtgctg gaagagtaca
    56701 tcctggtgcg ctttatcgac agcgacagcg ttcagccgac gcaggcagaa ctgacggcgc
    56761 atccgctgcc gcgttcatta agccgcctta acccggattt actgtacggc aatgtgctgg
    56821 caagcggcgt cggcgtgggt acgctgaccc tgttacaaag cgacagcctc gacagttatc
    56881 gggcaatccc cgccagtgcg caagattcca cccggctgga gcacagcctg gcaacgcttg
    56941 ccgagcaact gaaccagcaa ttgcgtgagc gtgacggcga aagcaaaact atcctcagcg
    57001 cccatttgtc gctgattcag gatgatgaat tcgcgggcaa tatccgtcgt ctgatgacag
    57061 aacagcatca agggctgggg gcggcgatca tcagcaatat ggagcaggtt tgcgccaaac
    57121 tttctgcttc tgccagtgat tatctgcgcg aacgtgttag cgacattcgc gatattagcg
    57181 aacagttgct gcatatcacc tggccggaac tgaagccgcg caacaatctg gtgctggaaa
    57241 aaccgactat tctggtagcc gaggatttaa ccccaagcca gtttttgagc ctcgatttaa
    57301 aaaatcttgc aggcatgatt ctggagaaaa ctgggcgcac ctcgcatacc ctgattctga
    57361 cccgcgcctc ggcgatcccg gtactgagcg gcctgccgct ggatgcgatt gcccgttatg
    57421 ccgggcaacc ggcggtgctt gacgcccagt gcggcgtgct ggcgattaac ccgaatgacg
    57481 cggtgagcgg ttattatcag gtcgcacaga cgctggcgga taaacgccaa aaacaacagg
    57541 cgcaggccgc cgcgcagctg gcctattccc gtgataacaa gcgtattgat attgcggcga
    57601 atatcggcac cgctctggaa gcgccaggcg cgtttgccaa cggcgcggaa ggcgttggac
    57661 tgttccgtac tgaaatgctc tatatggatc gcgacagcgc gccggacgag caggagcaat
    57721 ttgaagccta ccagcaggtg ctactggcag cgggcgacaa gccgattatc ttccgcacaa
    57781 tggacatcgg tggcgataaa agcattcctt atctgaatat tccccaggaa gagaacccgt
    57841 tcctcggcta tcgcgcggta cgtatttacc cggaatttgc tggcctgttc cgcactcaac
    57901 tacgggcgat tttgcgcgcc gccagttttg gcaacgccca gttgatgatc ccgatggtcc
    57961 acagtctcga tcagatctta tgggtgaagg gcgagatcca aaaagcgatc gttgagctta
    58021 agcgcgatgg cctgcgtcat gcagagacga ttacgctggg gatcatggtg gaagtgccgt
    58081 cggtgtgcta catcatcgat cacttctgcg atgaggtcga tttcttcagt atcggctcca
    58141 acgatatgac ccagtatctg tatgcggtcg atcgtaataa cccgcgcgta tcgccgctgt
    58201 ataacccgat tacgccatcg ttcctgcgca tgttgcagca gattgttacc actgcgcatc
    58261 agcggggcaa atgggtaggc atttgcggtg aactgggcgg tgaaagccgt tatctgccgc
    58321 tactgcttgg gctgggcctg gatgagctga gtatgagtag cccgcgtatt ccggcggtga
    58381 aaagccagct tcgtcaactg gatagcgagg cgtgtcggga actggcgcgt caggcatgtg
    58441 aatgccgcag tgcgcaggaa attgaagcgt tactcaccgc ctttacgccg gaagaagacg
    58501 ttcgcccact gctggcgctg gagaatatct ttgttgatca ggattttagc aataaagagc
    58561 aggcgatcca gttcctgtgc ggcaacctcg gcgttaacgg gcgcactgaa catccgtttg
    58621 agctggaaga agatgtctgg cagcgggaag agattgtcac caccggcgtt ggttttggcg
    58681 tagcgatccc gcacaccaaa tctcagtgga tccgtcattc cagtatcagc attgcccggc
    58741 tggcgaaacc ggttgactgg cagtcagaaa tgggcgaagt cgaactggtg atcatgctga
    58801 cgctgggtgc taacgaaggg atgaatcatg tgaaagtctt ctcgcagctg gcgcgtaaac
    58861 tggtgaataa aaacttccgc cagtcgttgt ttgccgcgca agatgcacaa agtatcctga
    58921 cgctgctgga aacagaatta accttctgac gttagccctg aaaacgggcg ctgtactctc
    58981 ccggcgtcag accaaactga cgccggaaaa cgcgacaaaa atagtcgcta tccggaaaac
    59041 cgcaacgctg tgccacttca tgaattgaca gatgatattt ctgcaaaatc atcctcgcct
    59101 tcgccattcg cacccaacgc acatactcga taaaacgcat cgttccatgc tgagcaaaca
    59161 gttttgacag atgattgggc gtgatattaa aaaactgcgc cacgctctcg cgggtgagcg
    59221 gctgggcata attatcctga acccagttac aaatgctgtg atagagaaaa tcgccgcgcg
    59281 gttgcgaatt acccggcggc atattcacca cgttgcggca aagatgcagc aaacttaaca
    59341 ccagtggctg aatgatattc tgctccagcg gcgaacggct aagatgggtg agggcggtaa
    59401 gcatcgcttc accttcaccg cgttgcaggc tgggaagctg gatctggcga gcaggatgca
    59461 acaacgacgt ggtgcggcta tcgtaaaacg ataacccaag ccaggtcggt gcgaacacca
    59521 ggcttaacag catcaccggt ttgttgttga tcggtaaatt agccgcgcgt gccggaataa
    59581 acagcatttc tccttccacc attcgctgct gctttgcttc cagcaggttg ccgtattcgc
    59641 cgcgcagcac gatatccaga cggggaaaat caatacataa actgcccacc ggtaacgagg
    59701 caggctggct ggcaaaccag acgcgcccca gtagttgcgg gttaagcacc aggccgctga
    59761 acagatcggc gaaaaattgt tgatcggcgg gcaagcctgg tgctttcatg aaagttcccg
    59821 attaaagatg cattaactgg cgaaattctt tcactttgct gcgactgacc ggcacttcaa
    59881 aatccagatc tttcagtcgc agaatgtagg tgttattaaa ccacggttcg atttcgcgta
    59941 ttttgttcag attgacacaa aatgagcgat ggcagcggaa aaaatgcgac ggcggcagtt
    60001 tgctgcaaaa ttcggtaatg ttcatcggca ttacgtagga ttcacgccgc gtatagacaa
    60061 aggtcatttt ctcgtgcgct tcggcgtaat agatatcgtt aattggcgtg acgattattc
    60121 gctcatcttt caccagatta atggtgtcat tctcacgcgt taccgtcgcg gcaggcgtac
    60181 tgctggtctg ctgttgttgc caggccgctt ccagtttttg cagcatcccg gtaatacgtg
    60241 actcctgata cggtttgaga atgtagtcaa acgcctccag ttcaaacgct tctaccgcat
    60301 gttctttcca cgcggtgatg aacacaataa acggtttatt ggcgaactgg ctgatgtttt
    60361 gcgccagcaa cacgccatcc agcgacggaa tattgatatc cagaaaaatg gcgtcgacgc
    60421 ggttatgctg caaaaacttc aacacgtcca gaccgtcgtc aaaggtgccg acaatctcca
    60481 tctggctgtg ctctttaatt agccagctca gttcctgttg tgccaggaat tcgtcttcaa
    60541 caatgatgac tttcacaata tcactccggc tcaaagcaat aacgtagcct gtgaggcgac
    60601 tggggtgcgt tggttaggaa tgtaaaacgc aatttccgtc cccggctcca ggcggcggat
    60661 atgcagcccc tcgccataca ataacttcac gcgatgatgg acattcagca ggccaatttt
    60721 attgcccggc atttcattcg cttcgacccg ctcaatcacc tttggatcga tgccgtgccc
    60781 ggtatctcgc accgcaatgc gtacccgatt tccgcactct gcaacgctga tggtgacaac
    60841 gcctttacct ttgcaaggct gaataccgtg gacaatggcg ttctccacca acggctggat
    60901 cagcaggctg ggaatgcagc aattcacctc ttcatcaata tcatagataa ccgtcagctt
    60961 gtcaccaaag cgggcctgct caatggcaat atagtcttta atttgataca gctctttttt
    61021 gatatcgatt tgctcatcgt cttttaattc aatgttatag cgcagataac gcgacagatt
    61081 aaagatcaac tggcgagcgg tatccggatt cagacggatt gacgatgaaa tagcgttcag
    61141 agcgttaaac agaaaatggg gattaatttt gctttgcagg gcgcgaagct ctgccttatt
    61201 tgccatttca cgtagctgct cggcgcgtga aacctccagt tgcgtggaga tgatttgcga
    61261 cagaccgacc gccatttcct gtaatgacga ggtgatctga tgcgcgtggc agtagtaaat
    61321 tttcagcgtt ccggtcacga cccctttctc ccacaatggg atcaccagca tggaatgaat
    61381 ctctggtgtg cggtgggctt catcattgtt tttaatgatg atttttccgt aattcatcgc
    61441 ctgacgggta gtcgggctaa tgaagtcatc gccattctga tagttatgtt cacccacgcc
    61501 aacataggcc agcacatgat cggtattagt aatcgccacc gcatcggcgt gaatgtcatc
    61561 gcggataatt tcgcagacct tgcgtaatga ctcgctattg acatggcgaa acagcggtag
    61621 cgttttgttg gcgatatcca gcgccagctt ggcctgccgc gcggcgctgg cctctttttc
    61681 gccctcaacg ctttgcacca gaagcacaat aaagccgata cagacgctac cgaggatcat
    61741 tggaatgccg attttagaga cgatatcgat ccccaacgcg gtagttggtg cccagacaat
    61801 taccagaatc atggtcaacg tctcacacaa catgccgccg agaataccga cgcgccagcg
    61861 ctgtgctttg gggattttca ggttgatcca gcccgatata caacccgcca gaatgctggt
    61921 gataaagcag gggatcgccg tcacgccgcc gatatcaatt aaataccggt gaatacccgc
    61981 aatcacgccg gtaatgatgc ctacccacgg gccaaacaga atcccgccgg acatcaccgc
    62041 gataatacgt acgttcacca gcgagccttc tacgggaacg ccggaccagg tgctgaacag
    62101 ggcgaacagc gagaaaatgg cggtaacggc gagcaattct tttggggagt gcgccgactt
    62161 gtgcaacagt tcgcgaaaca ggcggatacg gatgagaaag aacaggcaga taagcattaa
    62221 cgctgcccga tcgaagaccg ccagcagcat gttgaatatt tcgtgcacgg gtgactctga
    62281 ataaggatta aacctctatg ataggtaacc tgaaggctga tgaccagcag gccgtttttg
    62341 aggaaaaacc ataaatttaa aaggggattg aaagataaat ggtgtattag caatgtatct
    62401 cttgataatg cacttctggt tggtaaattt attgaaatta taatgttaat aaaattggtg
    62461 gtaattttct tgttagcgta gaaaaatttc ctgtttttga agtggagttc ctcatttaaa
    62521 gccattgata aaaatgttct aaaacgctgg acagacagaa attaatcagg ctattttcaa
    62581 aacagccacc attgtggcag cgtcgctcgg acggtccggg cgctaacgtt aatctgagga
    62641 aattatggct gacactcgcc ctgaacgtcg ctttacgcgc attgatcgtc tcccgcccta
    62701 cgtttttaac atcaccgctg aactgaaaat ggctgcgcgt cggcgcggcg aagatattat
    62761 cgatttcagc atgggtaacc cggacggtgc gactccgccg catatcgtcg aaaaattatg
    62821 tactgtggcc cagcgcccgg acacgcatgg ttactccact tcacgcggca ttccgcggtt
    62881 acgtcgcgcc atttcccgct ggtatcagga tcgctacgac gttgaaatcg acccggaatc
    62941 agaagccatc gtcactattg gttcgaaaga gggcctggcg catctgatgc tggcgacgct
    63001 ggatcatggt gacacggtgc tggtgccgaa tccaagttac ccgatccata tttatggcgc
    63061 ggtgattgcc ggggcgcagg tacgctcagt gccgctggtg gaaggtgtcg atttcttcaa
    63121 cgaactggag cgcgccattc gtgaaagtta tccgaaaccg aagatgatga tcctcggctt
    63181 cccgtcgaat ccaaccgcgc aatgcgtgga gctggagttc ttcgaaaagg ttgtggcgct
    63241 ggcgaaacgc tacgatgtgc tggtggtcca cgacctggcc tatgccgata tcgtctacga
    63301 tggctggaaa gcgccgtcaa tcatgcaggt accgggtgca cgcgatgtgg cagtcgagtt
    63361 ctttacgctg tcgaaaagct acaacatggc gggctggcgt atcggcttta tggttggcaa
    63421 caaaacgctg gtcagcgccc tggctcgtat taaaagctat cacgattacg gcacctttac
    63481 gccgttgcag gtggcggcga ttgcggcgct ggagggcgat caacagtgcg tgcgcgacat
    63541 tgccgaacag tacaaacgcc gccgtgatgt attggttaaa gggctgcatg aagcgggctg
    63601 gatggttgaa atgccgaagg cttcgatgta tgtctgggcg aaaatcccgg aaccatatgc
    63661 ggccatggga tcgctggaat ttgccaagaa gctccttaac gaagcgaagg tttgtgtctc
    63721 gccggggatt ggctttggcg actatggtga tacgcatgtt cgctttgcac tgattgaaaa
    63781 ccgcgaccgt attcgtcagg cgattcgtgg gattaaagcg atgttccgtg ccgacggttt
    63841 actacccgcc agcagcaaac atattcacga aaacgcggaa taaacaccgt cgtcagaaat
    63901 gcaaacagga gcctgatggc tcctgttttt cattgcacag tttgctttta gatcatcagg
    63961 gcaaaagtac cggcccaaac gatgaccata aaagaaatgc ccataaagaa atatttcact
    64021 ttacatctct ccgcgtaatt actgttacgc ctgaatgact tcattacacc tgatacagtg
    64081 agttgacacc gggcaaagcg accttaaaag ccctgtattt taccgtgggc atcaccccag
    64141 aattctgagc taaactgctc gagacggcgc taatgtacgc ctaaaactga aacgagacaa
    64201 gagacatttt cttatttact tttagtaact tacaagtgtc tcatatcggc gacagtttaa
    64261 tttctgacgg taataattag atattcagcg aaggaaaaaa ccttaagaaa aaagccataa
    64321 aaaccatgag gttattatgg ccgatttgag gagggaaaga gtaagagcag tttgttaaat
    64381 gtacaacgac gattctccca ccgggcgcgt tttaaagcga cggtggatcc agaggtactg
    64441 ctccggtgcg cgcatgatct ctttctcgat aatcttgttc atataggcag cggcttgatt
    64501 ttcatctgtc gggtagcctt ccatctctgg ggtgatgaac aaacgatatc cgctgtaatc
    64561 cgcttttctt accatcgtta cggtcaacat ggctgcgcca gagagacggg agagaacata
    64621 ggtgccattg gttgtggcga cattttccac cgcaaagaac ggcgcgaagg agctgccttt
    64681 acgaccataa tcctgatcgg gagcaaacca taccgcttca cctttcttca gtgcaccgac
    64741 aatgccgcgc agattatttc tgccgatcat cgctttgtta gagcgcatgc gcccacgggt
    64801 ctgcacccat tccatcagct gattattatg tggacgatag gtagccatca ttggttggca
    64861 cagtcccatc acgcggccgc ccagttccag cgacataaaa tggacgccga caaccattac
    64921 gccgcgattt tgcatttgtg cgcgtttaag gttatccaac ccttcaacat caaaccattt
    64981 acgtacgcga ctgtcgggcc agaaccatgc catgccggtt tctaccagcg ccatgccgag
    65041 tgaacgaaag ttttcggcaa tcatcttctc gcgttcttcc gcagaatgct gcgggaaaca
    65101 aagttccagg tttttacggg cgatagattc acgacgtttc aggaatggtc gcgccattgc
    65161 gccaatacgc gtgccgagaa agcagagaac aggataagga agctgtaccc aaagccagag
    65221 tacaccaagc ccaaaccatg tgagccagta gcgaggatgt agaaactcgc gggaaaattt
    65281 gcattgtgga aacatagtta ccttctgtag atacgaattg ttgtatcgca gaagattcca
    65341 tgtcgacagc ggtacactca attggttggg ccatcatgta cgacaatggt tcctgtaaag
    65401 agtgtaaaga ctagtcagga cataaccacg aggagcatga atgcggtatc tgtactttat
    65461 cactcgctga ctgctttcag ctactgatta ttaatcgtgc aaagtaaata tttttccgta
    65521 cacaacagtc ttgcatttaa catgctgttt tcattgatgc ttagcaaaaa ttgccagagg
    65581 ttaaggattg tcttactatt aaagtgactg aagttacagg ttttttagcg cggcaggagg
    65641 ttgagctatg atttatttat ggatgtttct cgccctgtgt attgtctgcg taagcggcta
    65701 catcggtcag gtactgaacg tggtatcggc ggtctcttct ttcttcggca tggtgatcct
    65761 cgctgcactc atttattact tcaccatgtg gttaactggc ggtaatgaac tagtgacggg
    65821 gatatttatg tttcttgccc cggcttgtgg cttgatgatt cgctttatgg tggggtatgg
    65881 caggcgatag tgggtcagga tggctcattt gggcaggtgt tcacatcagc atcagacatg
    65941 attgttcacc tgccatgtga cagacgctat cgcacaggat gaataagata attcgccttt
    66001 gtcggataag taggcgtgat ttcttctgtg aggtttaatg gtggcgcatc ttctctcatc
    66061 ttaaacaaaa tcgcctgtga tggctgaagt tttgtagagt gtgtggttgt catcgctgat
    66121 gttttttcag tctctttata gctcctctca cttacaccac cagaaatgga aaatgcaaaa
    66181 acattgcatg ccataataga gaccaataaa aataagctga aaagtattaa gtttactttc
    66241 atagaaagtt atcctgaaaa tggttcactg agaacctacg atgttatggc
//
LOCUS       DAZFKM010000009        63956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFKM010000009 DAZFKM010000000
VERSION     DAZFKM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..640)
                     /locus_tag="ACHKAY_000657"
     CDS             complement(<1..640)
                     /locus_tag="ACHKAY_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699379.1"
                     /translation="MRKLLLPLLFMAGTVNAASSVKEICTDYTKYLGHVYGFAVSEDE
                     SMRKKLLSDMKRLKLSEAMVQQELYKVSTNANAKYQYSRLLNPDANEINRSTFDYMVK
                     ACETAPDFAIPSWGVLVASNAVNKEDVGRNGIDSIRNAPGMRHQNVQGTLEERARGPG
                     TNSPMGNLSPEELQEYNQRMEQYEKAAREQMEQQKNGSLNTFQQGLKALNLPY"
     gene            1067..1288
                     /locus_tag="ACHKAY_000658"
     CDS             1067..1288
                     /locus_tag="ACHKAY_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5699380.1"
                     /translation="MVNANPCTRPEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGVL"
     gene            1285..2328
                     /locus_tag="ACHKAY_000659"
     CDS             1285..2328
                     /locus_tag="ACHKAY_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5699381.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRVTGKNLNVEKPAPVTSATP
                     TPAVNVIPNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIEKILDELEGEFYTKSAK
                     SHGTRAGRSFEVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPAPSIPQTLPE
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSVANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAIG"
     gene            2493..3293
                     /locus_tag="ACHKAY_000660"
     CDS             2493..3293
                     /locus_tag="ACHKAY_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5699382.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPKSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            3323..4168
                     /locus_tag="ACHKAY_000661"
     CDS             3323..4168
                     /locus_tag="ACHKAY_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5699383.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALTESKLKISSAATKVKAVLAKTLALFDFLTTPPYQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSGGAGTTRLKKLTSWIAEAKAKADNLRLSKKRTQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            4337..5041
                     /locus_tag="ACHKAY_000662"
     CDS             4337..5041
                     /locus_tag="ACHKAY_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO5699384.1"
                     /translation="MLNRRTFNVFCDESCHLLNDHNKVMVLGALWCPGTITKKIARDI
                     KELKLKHNLKPDFEIKWTKVSASKVEFYLDVVDYFFSNPALRFRGVVVPDKEQLDHAR
                     FHQDHNTFYYKMFFYVLKNIIESNNTYNIYLDIKDTLGIEKIEKLRGVLHNDRYDYNH
                     ESINRIQHIRSHEVQQLQLTDLFIGALGYVHRGMNSNAGKIQVINRIKSHTNRDLLKS
                     TLPTESKFNIFVWEAR"
     gene            complement(6053..6562)
                     /locus_tag="ACHKAY_000663"
     CDS             complement(6053..6562)
                     /locus_tag="ACHKAY_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFO5699385.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTVAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(6574..7032)
                     /locus_tag="ACHKAY_000664"
     CDS             complement(6574..7032)
                     /locus_tag="ACHKAY_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699386.1"
                     /translation="MAQNWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWE
                     GGTPPTFTLPVTFIALFDPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRQ
                     INIIDVAIQDISFDLDAPRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(7191..8006)
                     /locus_tag="ACHKAY_000665"
     CDS             complement(7191..8006)
                     /locus_tag="ACHKAY_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699387.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEQPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            complement(8016..9605)
                     /locus_tag="ACHKAY_000666"
     CDS             complement(8016..9605)
                     /locus_tag="ACHKAY_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699388.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYKDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            complement(9666..11372)
                     /locus_tag="ACHKAY_000667"
     CDS             complement(9666..11372)
                     /locus_tag="ACHKAY_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699389.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFNV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            complement(11597..12598)
                     /locus_tag="ACHKAY_000668"
     CDS             complement(11597..12598)
                     /locus_tag="ACHKAY_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO5699390.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            complement(12615..13811)
                     /locus_tag="ACHKAY_000669"
     CDS             complement(12615..13811)
                     /locus_tag="ACHKAY_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5699391.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            complement(13980..14351)
                     /locus_tag="ACHKAY_000670"
     CDS             complement(13980..14351)
                     /locus_tag="ACHKAY_000670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699392.1"
                     /translation="MAYVSISGYEPDAIRAQCELLKAMFMLRTKYPWETGRVCFPMEE
                     LTATIDSQLYHDNPEKFFSLLAGNGLNCVSGLGRSRIQHASLFGPFFYDRHGYFEAEL
                     PALTRAIDTIDFERLFAALSK"
     gene            complement(14454..14789)
                     /locus_tag="ACHKAY_000671"
     CDS             complement(14454..14789)
                     /locus_tag="ACHKAY_000671"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFO5699393.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIK"
     gene            complement(15052..15936)
                     /locus_tag="ACHKAY_000672"
     CDS             complement(15052..15936)
                     /locus_tag="ACHKAY_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO5699394.1"
                     /translation="MLEENKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            complement(16270..16662)
                     /locus_tag="ACHKAY_000673"
     CDS             complement(16270..16662)
                     /locus_tag="ACHKAY_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699395.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            complement(16674..16814)
                     /locus_tag="ACHKAY_000674"
     CDS             complement(16674..16814)
                     /locus_tag="ACHKAY_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699396.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            complement(16840..17262)
                     /locus_tag="ACHKAY_000675"
     CDS             complement(16840..17262)
                     /locus_tag="ACHKAY_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HFO5699397.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            complement(17302..18090)
                     /locus_tag="ACHKAY_000676"
     CDS             complement(17302..18090)
                     /locus_tag="ACHKAY_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699398.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQALNTGSESS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAVAALVPDSVLDELNKAAR"
     gene            18553..18837
                     /locus_tag="ACHKAY_000677"
     CDS             18553..18837
                     /locus_tag="ACHKAY_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699399.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGINRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            18830..19735
                     /gene="rdgC"
                     /locus_tag="ACHKAY_000678"
     CDS             18830..19735
                     /gene="rdgC"
                     /locus_tag="ACHKAY_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO5699400.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     AVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            19732..22773
                     /locus_tag="ACHKAY_000679"
     CDS             19732..22773
                     /locus_tag="ACHKAY_000679"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFO5699401.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALR
                     WPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDY
                     VVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYF
                     KRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIR
                     RWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHF
                     GELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQT
                     CGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKS
                     VGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIAGLRGGLDDERGALTLKYVELANAID
                     DKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFW
                     RWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPA
                     TVLFEFEGVRRNIAPSRGEGKETTRYTSNIAIRTCDDTNIIAMAHGQGGAEIKTDNSA
                     PTLTCNHEAPIVLLGDGRMRRLTPVECERLQGFPDWHTLIPTEKRKKVNSDELAYLHN
                     HYPDLSEEEAAMLAADGPCYKAIGNSMAIPVMRWIGDRITKAVCRQKEGSETKERKVK
                     PAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQENLLNDVN
                     ADLINFYKTLQREAHSLITLAHRFFQDYNTQEGYLAVRNAFNKQVYDDLHRAAAFLFL
                     NRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDFAAVIEAA
                     GEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKVLITNSGA
                     PNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAHDVLGILL"
     gene            23446..23520
                     /locus_tag="ACHKAY_000680"
     tRNA            23446..23520
                     /locus_tag="ACHKAY_000680"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23478..23480,aa:Asn,seq:gtt)
     gene            23526..23612
                     /locus_tag="ACHKAY_000681"
     tRNA            23526..23612
                     /locus_tag="ACHKAY_000681"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23560..23562,aa:Tyr,seq:gta)
     gene            23617..23692
                     /locus_tag="ACHKAY_000682"
     tRNA            23617..23692
                     /locus_tag="ACHKAY_000682"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23650..23652,aa:Thr,seq:tgt)
     gene            24783..25610
                     /locus_tag="ACHKAY_000683"
     CDS             24783..25610
                     /locus_tag="ACHKAY_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5699402.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMDLEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            25638..25713
                     /locus_tag="ACHKAY_000684"
     tRNA            25638..25713
                     /locus_tag="ACHKAY_000684"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25671..25673,aa:Ile,seq:cat)
     gene            25994..27358
                     /gene="dnaB"
                     /locus_tag="ACHKAY_000685"
     CDS             25994..27358
                     /gene="dnaB"
                     /locus_tag="ACHKAY_000685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5699403.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            27358..28356
                     /locus_tag="ACHKAY_000686"
     CDS             27358..28356
                     /locus_tag="ACHKAY_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699404.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQFHRPYSPGVESPEDYVEAVLTAATSCSAKAILAPGLRYFTTVPREIGVQLRRRFT
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQDLFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLITIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGNG"
     gene            complement(28403..29035)
                     /locus_tag="ACHKAY_000687"
     CDS             complement(28403..29035)
                     /locus_tag="ACHKAY_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFO5699405.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEVRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(29028..30044)
                     /locus_tag="ACHKAY_000688"
     CDS             complement(29028..30044)
                     /locus_tag="ACHKAY_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5699406.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            complement(30046..30831)
                     /locus_tag="ACHKAY_000689"
     CDS             complement(30046..30831)
                     /locus_tag="ACHKAY_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HFO5699407.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRTNQSFREFLKRTPRDRITLGTFSDVKKWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            complement(30818..31546)
                     /locus_tag="ACHKAY_000690"
     CDS             complement(30818..31546)
                     /locus_tag="ACHKAY_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5699408.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEDD
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRNKNAPETAPEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            complement(31550..32767)
                     /locus_tag="ACHKAY_000691"
     CDS             complement(31550..32767)
                     /locus_tag="ACHKAY_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5699409.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADICKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            complement(32777..33037)
                     /locus_tag="ACHKAY_000692"
     CDS             complement(32777..33037)
                     /locus_tag="ACHKAY_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699410.1"
                     /translation="MCESNDKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAG
                     LEDESQGMTVGKKLKLPPATWIREMIRHFQYDGQVIGTLSIA"
     gene            33301..33546
                     /locus_tag="ACHKAY_000693"
     CDS             33301..33546
                     /locus_tag="ACHKAY_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699411.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            33549..34127
                     /locus_tag="ACHKAY_000694"
     CDS             33549..34127
                     /locus_tag="ACHKAY_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFO5699412.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLYAA"
     gene            34194..34349
                     /locus_tag="ACHKAY_000695"
     CDS             34194..34349
                     /locus_tag="ACHKAY_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699413.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            34453..34953
                     /locus_tag="ACHKAY_000696"
     CDS             34453..34953
                     /locus_tag="ACHKAY_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699414.1"
                     /translation="MFDDIYALYAGDLSWWKQYGSTIPGGKFRKVTANLAAAKSFSLE
                     YRRYCGPAEGVNSGAQAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQV
                     SISKWQQQFLDTRKKHADLHILNASRSSAIQCFPRINLEAVIALLSSAVAQAPQTLLR
                     RAECRL"
     gene            34851..35477
                     /locus_tag="ACHKAY_000697"
     CDS             34851..35477
                     /locus_tag="ACHKAY_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699415.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNMNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            35474..36151
                     /locus_tag="ACHKAY_000698"
     CDS             35474..36151
                     /locus_tag="ACHKAY_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO5699416.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            36148..36849
                     /locus_tag="ACHKAY_000699"
     CDS             36148..36849
                     /locus_tag="ACHKAY_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699417.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            37151..38413
                     /locus_tag="ACHKAY_000700"
     CDS             37151..38413
                     /locus_tag="ACHKAY_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699418.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLQIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLAEAELPFEPEFIDELANKGLHSLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            38486..38992
                     /locus_tag="ACHKAY_000701"
     CDS             38486..38992
                     /locus_tag="ACHKAY_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HFO5699419.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLSGIYTLWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTLMVREY
                     ENALLHQQ"
     gene            39187..39912
                     /locus_tag="ACHKAY_000702"
     CDS             39187..39912
                     /locus_tag="ACHKAY_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699420.1"
                     /translation="MKGEVKKRGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMVRKIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LLEITNVRVERLNDISECDARDEGVPPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWSANPWVWVIEFERIQ"
     gene            39952..40143
                     /locus_tag="ACHKAY_000703"
     CDS             39952..40143
                     /locus_tag="ACHKAY_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5699421.1"
                     /translation="MNKASPAELRASLEMAHSLAQIGVRFVPIPVETDEEFHRLAASA
                     AQKLEIMAAKVEKAEGATK"
     gene            40140..40355
                     /locus_tag="ACHKAY_000704"
     CDS             40140..40355
                     /locus_tag="ACHKAY_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699422.1"
                     /translation="MSKPTDEEIVRVLEEHGRCMTYVVTNWLRDKYRTLKTAYVLRRL
                     KKLEFDGKVKRVNSSYIRQICWEASSE"
     gene            40348..40992
                     /locus_tag="ACHKAY_000705"
     CDS             40348..40992
                     /locus_tag="ACHKAY_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699423.1"
                     /translation="MSNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKEQNRRKKSRSGKVRWNCPHCGKITWQYSPYDFEGCSDYNCEGWRE"
     gene            40989..41582
                     /locus_tag="ACHKAY_000706"
     CDS             40989..41582
                     /locus_tag="ACHKAY_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5699424.1"
                     /translation="MTIDYQALRDAAVAVETEPMHQNFVAFRMAFTPSVALALLDEIK
                     RLEDTNIDAMCRIAELETNLAALVAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIIND
                     ALHHSETPATDAFLAEIRAEARNEGINYTASRLAAAFNHGFINKSLREVFDVTRMILS
                     AKEELANEAHPIDGLSGEYAEKSLEEWAEQIRKGSSQ"
     gene            41579..41869
                     /locus_tag="ACHKAY_000707"
     CDS             41579..41869
                     /locus_tag="ACHKAY_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5699425.1"
                     /translation="MNIDTTITIDTLLNTGLALLGWLFIMSRTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQERAEKGESA"
     gene            41866..42570
                     /locus_tag="ACHKAY_000708"
     CDS             41866..42570
                     /locus_tag="ACHKAY_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699426.1"
                     /translation="MKFSKFSELVNRILFNNHSHRRDMDVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGTVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSIELDAAKQRIAELEGNCAALAAENAAMHETIEAVRGVADNSSGIAGWHLNGDIATW
                     EEILPEINDIETPATDAFLAEVRAQGVEMLAKNHQSIVNALKGDSLFSDGEYRHAAIV
                     SAAVYFAAELRKGGNQ"
     gene            42567..43223
                     /locus_tag="ACHKAY_000709"
     CDS             42567..43223
                     /locus_tag="ACHKAY_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5699427.1"
                     /translation="MSEINYQALREAAERATPAMERLLMLPVDDDLLSEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKSRDQENEEIALTEGKLLIENGRLVADT
                     LRHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIA
                     ELEAREISLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGEEHGNKTRR
                     "
     gene            43201..43557
                     /locus_tag="ACHKAY_000710"
     CDS             43201..43557
                     /locus_tag="ACHKAY_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699428.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRILRQEERERRRNIEKLIQIE
                     RNVKARIRRVTKSRTE"
     gene            43606..43818
                     /locus_tag="ACHKAY_000711"
     CDS             43606..43818
                     /locus_tag="ACHKAY_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699429.1"
                     /translation="MSNAQKVINAEKYNEWVKKFSEQIFKITGDENAAKNELEPWTPE
                     GVDPNYCWWDVDPVDAANEAMSYHND"
     gene            44019..44717
                     /locus_tag="ACHKAY_000712"
     CDS             44019..44717
                     /locus_tag="ACHKAY_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5699430.1"
                     /translation="MHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVFNGEYGDAY
                     QGAREDLSIWKRRALEADEHVRRLEQINDHMVKEAQGESRMGEPVIREPAPVVPEEAT
                     PENVEMLSGYVSTYKLTDSERDIAAEIWNACRAAMLQAGNFRENKGSSTNNFREISET
                     STNYPVTPDGWISCSERMPAQDDWILIYSKHGEYMAGQVQGEYVELSDGTLSWLGNAL
                     FWMLLPEPPQEAGQ"
     gene            44800..45033
                     /locus_tag="ACHKAY_000713"
     CDS             44800..45033
                     /locus_tag="ACHKAY_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699431.1"
                     /translation="MGKLTFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIQKMELLTH"
     gene            45212..45505
                     /locus_tag="ACHKAY_000714"
     CDS             45212..45505
                     /locus_tag="ACHKAY_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699432.1"
                     /translation="MWRGNSHGKSQMILTEYKLDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            45512..45886
                     /locus_tag="ACHKAY_000715"
     CDS             45512..45886
                     /locus_tag="ACHKAY_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699433.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPQTEVGELHLWLLGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRKKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            45868..46548
                     /locus_tag="ACHKAY_000716"
     CDS             45868..46548
                     /locus_tag="ACHKAY_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699434.1"
                     /translation="MAQKEMTTLTTAYLQQLVFFAGEATCHPDANYLLEFERLASPGI
                     VLELAQQVLALRQKEQHESNTCRLNFEQWLEQQRGKIDVDCGCVSTETFMHWLRVAYE
                     AGNYPDIPDSSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTRQLREKISRV
                     LGDVITPDHHKQAEKSGLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVADKH
                     EAGHANES"
     gene            46821..47099
                     /locus_tag="ACHKAY_000717"
     CDS             46821..47099
                     /locus_tag="ACHKAY_000717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699435.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQDWVSNDDLAEKITSLVDKILQIGNEITLIHW"
     gene            47221..47610
                     /locus_tag="ACHKAY_000718"
     CDS             47221..47610
                     /locus_tag="ACHKAY_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5699436.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            47683..47904
                     /locus_tag="ACHKAY_000719"
     CDS             47683..47904
                     /locus_tag="ACHKAY_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO5699437.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            47904..48284
                     /locus_tag="ACHKAY_000720"
     CDS             47904..48284
                     /locus_tag="ACHKAY_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO5699438.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            48289..48468
                     /locus_tag="ACHKAY_000721"
     CDS             48289..48468
                     /locus_tag="ACHKAY_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HFO5699439.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            48496..49539
                     /locus_tag="ACHKAY_000722"
     CDS             48496..49539
                     /locus_tag="ACHKAY_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5699440.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQLFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAATQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
     gene            49628..50080
                     /locus_tag="ACHKAY_000723"
     CDS             49628..50080
                     /locus_tag="ACHKAY_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HFO5699441.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            50166..51359
                     /locus_tag="ACHKAY_000724"
     CDS             50166..51359
                     /locus_tag="ACHKAY_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5699442.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNDQHAGNHIAAEIAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGASNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            51359..52843
                     /locus_tag="ACHKAY_000725"
     CDS             51359..52843
                     /locus_tag="ACHKAY_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO5699443.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNATIEEASKIPVGINSAGQWRVMTKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            53065..53184
                     /locus_tag="ACHKAY_000726"
     CDS             53065..53184
                     /locus_tag="ACHKAY_000726"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO5699444.1"
                     /translation="MVVLAGLPKGRPVPFDAGSLNPVSATAIEIETSRGSSLT"
     gene            53203..53424
                     /locus_tag="ACHKAY_000727"
     CDS             53203..53424
                     /locus_tag="ACHKAY_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5699445.1"
                     /translation="MATVPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            53421..54539
                     /locus_tag="ACHKAY_000728"
     CDS             53421..54539
                     /locus_tag="ACHKAY_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5699446.1"
                     /translation="MRTSLVTREEMIEAIEQHTSCISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKANNVKLEKIHAPSVQES
                     ELVVVQNQSDEIPVLEWQGVRVVTTETLARGYGTETIRIRQNHHENKVRFVEGKHFFK
                     VEGESLRELKHRVALNYSVKIARNVRSLTLWTERGAARHAKMLETDQAWAFFEKLEDS
                     YFRQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVS
                     VAKGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVL
                     DDEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(54615..55634)
                     /locus_tag="ACHKAY_000729"
     CDS             complement(54615..55634)
                     /locus_tag="ACHKAY_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HFO5699447.1"
                     /translation="MADVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKAMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYSKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEETAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKIKEVPQAANVQAPRETSIITFNNS
                     INPFN"
     gene            complement(55627..57336)
                     /locus_tag="ACHKAY_000730"
     CDS             complement(55627..57336)
                     /locus_tag="ACHKAY_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5699448.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWTIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     NNG"
     gene            complement(57937..58578)
                     /locus_tag="ACHKAY_000731"
     CDS             complement(57937..58578)
                     /locus_tag="ACHKAY_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HFO5699449.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLRPLINIIHGTQTLAQKQVPQGELTGKGNYL"
     gene            complement(58767..59327)
                     /locus_tag="ACHKAY_000732"
     CDS             complement(58767..59327)
                     /locus_tag="ACHKAY_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HFO5699450.1"
                     /translation="MKTIEQKLEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DSIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISNEVSLHHIAGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMANILH"
     gene            complement(59573..59884)
                     /locus_tag="ACHKAY_000733"
     CDS             complement(59573..59884)
                     /locus_tag="ACHKAY_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HFO5699451.1"
                     /translation="MRKDFKIDGKYVVLSVSSQIQSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVMAEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            complement(59935..60966)
                     /locus_tag="ACHKAY_000734"
     CDS             complement(59935..60966)
                     /locus_tag="ACHKAY_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5699452.1"
                     /translation="MTNSLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSDHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            complement(60963..61358)
                     /locus_tag="ACHKAY_000735"
     CDS             complement(60963..61358)
                     /locus_tag="ACHKAY_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699453.1"
                     /translation="MFNGLTDAKVNALLNDAKQKAANPGTNENERAMAVAIWELAEEV
                     KRNRLLYTRVYQQFDGASWIDIEEAEYATCKKEKKIVRILHKAATPITVEIPDHRYFE
                     NDDLPGDVIRIIDEILHEQGLTVKITHNK"
     gene            complement(61378..61599)
                     /locus_tag="ACHKAY_000736"
     CDS             complement(61378..61599)
                     /locus_tag="ACHKAY_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HFO5699454.1"
                     /translation="MGYSAAKVSTHLELEKNRGYWRAKWFERDSYQLSLSRGEEKIER
                     TRGRWRFYDENHNQVKAEPILYTLLKTII"
     gene            62204..62413
                     /locus_tag="ACHKAY_000737"
     CDS             62204..62413
                     /locus_tag="ACHKAY_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HFO5699455.1"
                     /translation="MAFIQPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQLDSQ"
     gene            62524..63375
                     /locus_tag="ACHKAY_000738"
     CDS             62524..63375
                     /locus_tag="ACHKAY_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HFO5699456.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DTPESAQ"
     gene            complement(63408..>63956)
                     /locus_tag="ACHKAY_000739"
     CDS             complement(63408..>63956)
                     /locus_tag="ACHKAY_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5699457.1"
                     /translation="WTERGAARMSKIVDTNEAWAFFEKLEDSYFRQKEQQPVAIPQTL
                     PEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSVAKGILIGNFAKVVGL
                     KQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDDEDGYQIRLTPQLTG
                     KGQQWLTRKLLDAGLLKPVAAE"
BASE COUNT        16946 a        14724 c        15740 g        16546 t
ORIGIN      
        1 catagggtaa attaagcgct tttaaacctt gctggaaggt attaagtgag ccgttctttt
       61 gttgttccat ttgttcgcgt gccgctttct catactgctc catccgttgg ttatactctt
      121 gcaattcctc tggtgagagg tttcccattg gggagtttgt acccggcccc ctggcccgct
      181 cttcaagcgt accctgcacg ttttgatggc gcattcctgg ggcatttctg attgagtcaa
      241 tgccatttct tccaacatct tctttattaa cggcattgct cgccaccagc acaccccagc
      301 tagggatagc aaaatcagga gcggtttcgc atgcctttac catataatcg aaagtgcttc
      361 gattgatctc atttgcatcg gggtttaata ggcgagaata ttggtattta gcatttgcgt
      421 tggttgagac tttatacagt tcctgctgca ccatcgcttc agaaagttta aggcgcttca
      481 tatctgacag taacttcttg cgcatggatt cgtcttcact gacagcaaag ccgtaaacgt
      541 gcccaaggta tttcgtataa tcggtacaaa tctcctttac gcttgatgct gcattaacag
      601 tcccagccat aaataataac ggtagtaata gttttctcat tataacctca cctgccttat
      661 aactcattta gggtacatat tttcgccttt aaaaaaaaga ggttattaga tccaattgtg
      721 catttattaa acaaacaatg ctctaataaa tttgtatttt taagtcacga atgctatctt
      781 ttcgcatcat attgaccttt taatcgttca ggcttatagt tccgccgtcg tagcaaattc
      841 tgcgaccggg tttgacagcc tgaatgttag tgcggacaac cgcagatttc cgatattgcg
      901 gtatttttgt gtccgtaaac cgcgttacgc ccgaattatg gtggggcgtg atggggaggc
      961 ttcggcctgc tggtttcact aacgccagtc tgtcaaccct gtcacgtcct gccacctgtt
     1021 tgacagcggg tagcaggttg ttaaacctgt tagtgaggcc gtaactatgg ttaatgccaa
     1081 tccttgcaca cgcccagaat tcatctggcg tttctattcc tgtaaaaaac accactatca
     1141 cttcgttatc gcagcaactg aagacgaagc acgctctcaa ttgcctgatg gcccctgcat
     1201 ttttactgcc cgtttttcaa ctaactcgcg caattcactt agttactgga acctcccctt
     1261 ctctgccgac gttcaggggg ttttatgaaa aaacctctcg tcacccgtaa tgacatagcc
     1321 gaagcaatcg ccctgcatac tgcctgtatg ccgacacggg agatccccgg cgcaattgcc
     1381 aactatttca tgataaccag gcgtttttat acccgaacag ataaggcagt gatcaacagg
     1441 ctactgatag ccgagatcag ggattatttg attgaacaag gacgtctacg ttacgcaacg
     1501 gtggcagcag aaatgagaaa ggaggcacat agagtgaccg gtaaaaattt gaatgttgag
     1561 aaaccagcac ctgttacgtc agctacgcca acaccagccg tgaatgtcat ccccaacacc
     1621 ggagacacaa tcgacagcca aacattgttg aagatggtca atgaggcacg caagttatgt
     1681 ggagaaccag aggttagaaa caataaattc atcgaaaaaa tactcgatga acttgaaggt
     1741 gaattttaca caaaaagtgc aaaatcccat ggcacaaggg ctgggcgctc ttttgaggtc
     1801 atcaccatga cctacaaaca agccttgcga gtcgccgcgc gcgagtcaaa agcggtccgc
     1861 cgttcgctga tcgacaaact ggaagaattg cagcaggcaa actcccctgc cccatcgatc
     1921 ccccaaacat taccagaagc tctacgcctg gctgccgagt tggcagaaca gaaaatgcag
     1981 ttggaacagc agctggtggc cgcagcccct aaagtcgatt ttgccgaccg ggtatcagtg
     2041 gccaatggaa tcctgatcgg gaactttgca aaggtcgttg gacttaagca aaacgccctt
     2101 ttctcatggt tgcgccagaa cggcattctc atggcttttg gtgcgcgcaa aaacgtaccg
     2161 cgccaacagt acatcaacgc cgggtatttc acagtgaaag aagtggtgct ggatgatgaa
     2221 aatggctacc agatacggct gacgccccaa ttaacgggta aaggccagca gtggttaact
     2281 cgcaagctac ttgatgctgg tttgttaaaa ccagtagcaa taggttaaca aaagaaaaaa
     2341 acctgccagc aaactggcag gtttctgagc agatcgacca acccgatctg gatcgagtta
     2401 gaaaaatttg ctctaataaa tttcgttttc taagtgcaaa aaatcaccat ttcgagctgg
     2461 tgattgaagg ttgatgcaaa tttggagaaa aaatgcaaca aacattcaat gcggatatga
     2521 atatatcaaa ccttcatcaa aatgtcgatc cttcaaccac tctgcctgtt atttgtggtg
     2581 ttgaaattac gaccgaccgc gctggccgtt acaaccttaa tgctctacac agagcgagcg
     2641 gactcggtgc ccataaagcg ccagctcaat ggctaagaac gctgtcagcc aaacagctca
     2701 tcgaagagct tgaaaaagaa actatgcaga attgcatagt ttcgttcgaa ggccgtggcg
     2761 gcggcacttt tgcccatgaa ttgctcgcag tggagtacgc aggctggatt tctcccgcgt
     2821 ttcggctgaa ggtaaaccag acatttatcg actatcgagc cggaagatta caacctgcta
     2881 ttccgaagag cctcccagaa gctctccgtt tggctgctga cctggcagag caaaagcaac
     2941 ggctggagca aaaaatgctg atggatgcac ctaaagtcga attcgccgaa cgcgttgcta
     3001 ccgccagcgg ggttctaatc ggcaactatg ccaaagtgct cggcctgggc caaaactatc
     3061 tcttcacctg gttgcgtgat aacggaattc tgatcgcaac cggtgaacgc aggaacgtcc
     3121 ccaaacaaga atacatatcc cgtgggtatt tcacccttaa agaaaccgtg atcgatacaa
     3181 gcaatggaag caggatttct ttcacgactc gtataaccgg caaaggtcag cagtggctga
     3241 tgaagcgatt gcttgatgct ggtgtgctgg tacctgtcgc ggcaacgcgc taacagacgt
     3301 agtaagaacc accagcattg taatgctggc taaagtcact ttcctgagct gtataacgat
     3361 gagcgatttt actttttctg gctatgaatt ggcctgcttt gtaacacact ccggtctatc
     3421 ccgtagcgcc gggcatatcc tgtcgcaatg tgcaaatctc gcggcaacaa ccagtgaata
     3481 cttcattcac aagcctcacc gcctgatcgc ggcagaaact ggttatagcc aatcaaccgt
     3541 cgttcgtgca ttccgtgaag ctgtaaacaa aggaattctg tctgtagaga ttgttatcgg
     3601 cgatcaccgt gaacgtcgcg ctaacctgta ccggtttaca ccatcctttt tggccttcgc
     3661 acaacaagct aaaaacgcgc tgactgaaag caaattaaag atctcttcag ccgcaaccaa
     3721 ggttaaagct gttctcgcta agacattggc tttatttgat tttttaacca cacccccata
     3781 tcaaaatgat accccctccc cctgtcagga tgacgtggca ataaagaata agaagtcaca
     3841 agttaaaaaa acaaaaagat cagtttccgg cggtgccgga acgaccagac tcaaaaaatt
     3901 gacttcatgg atcgctgagg caaaagcaaa ggctgacaat ctgcggttat ccaaaaaacg
     3961 cactcaaaaa catgagttca agcagaaagt agaggcggct gcgcggaaat atgcttacct
     4021 gaagaacaag cgttcgcctg atattggcgg gatatcaaac ttcgataacc taccgcattg
     4081 catgacggta aacgaagctc ttaatgcggt tttagccaaa aataaagata acgaacaatg
     4141 gggtataccg gcgggattca gagggtaatg aattgttcta attataacca tgcatacttt
     4201 caacgcctct agtttgccat gaggcaaact cataggcgtc ctggtaagag ggcactgttg
     4261 ccaaaactgg acgtcccatt attgcaatta ataaacaact aacggacaat tctacctaac
     4321 aataagtgga gttaacatgt tgaaccgaag aacatttaat gtattctgcg atgaatcctg
     4381 tcacctacta aatgatcata ataaagtcat ggtattgggt gcgctttggt gccctggcac
     4441 tatcacaaaa aaaattgcta gagacatcaa ggaattaaaa ttaaaacaca atctaaaacc
     4501 tgatttcgaa atcaagtgga ctaaagtatc tgcgtccaag gttgaatttt acttagatgt
     4561 cgtagattac ttcttcagca acccagcatt gcgctttaga ggtgttgttg ttcctgataa
     4621 agagcaactg gaccatgctc gattccatca agatcataat actttctatt ataagatgtt
     4681 tttttatgtc ttaaaaaaca taatagaaag taacaacaca tacaatatct acctagatat
     4741 aaaagatact ttaggcattg aaaaaattga gaaattaaga ggagttcttc acaatgatcg
     4801 ctatgattac aatcatgagt cgataaacag aattcaacat atacgatctc atgaagtgca
     4861 acaattgcag ctaacggatc ttttcattgg tgcgttaggc tacgttcatc gaggaatgaa
     4921 tagcaacgcg ggaaaaatcc aagtcataaa caggataaaa tcacatacaa acagggattt
     4981 actaaaaagc actcttccaa cagaaagtaa atttaatatt ttcgtgtggg aggctcgctg
     5041 atgcttcaaa tgccagattt attgtacttc aatggaagtt ggcaagagta tatagacgat
     5101 gtatattatg ttgtcagaga agacatttta atctctaata taacgtttaa gggtctccct
     5161 gttcggttac gttattcacc ggaatatgat gggaaagagt tcggattttg gcatttagta
     5221 tcagagggaa aaatagaaga agaacgaata cctgatcttg aacggtgtaa gcggatccgc
     5281 tggatcgcgc atatgataag gaattataac cattccgata tatcatgttg gtctgaaaga
     5341 cgaggaccaa ctgaagaatg ggtaatctgg aacgagtgtg aaaactacgt tgttgtgcta
     5401 tccgcacgta gagactattg gcttcttaaa acagcctatg ttgtaaccta taacagtaaa
     5461 atcagaacac tcaaacaaag cagaaaaaga gcgcttggga cataaaaaag ctgaacccga
     5521 cacatattgc tatgtatcgg gttcgatcgc tctttctaca catggtagat gagtaaggca
     5581 aatctaaccg atctacttta gcttttcaat agctagctat aactttccgc acatattctt
     5641 ctgataattc cagtatggct gttgggtttt cttatgcttt tctgcgcaga ggtgcacatt
     5701 tattcagcaa taataaatgt aattctttgt tattcctaag tatttcgaca caccaatatc
     5761 gtaacgtgtt cacttcaagt gtagccagca ccttatgtcg aagtcctccc tcccctgcac
     5821 ataacgatga ccgacattct gctaaggcac ataaccatgt attggcatca tagagaatcc
     5881 aataatgctc ctctgaaata gttttgcatc caattatttc gaatcttcgt tctatcgttt
     5941 caggagagta tgaaaaatca atttccataa tatgctcaaa atttcgctat accagcataa
     6001 tttaagcaca aaaaaagctc ccgcaggagc tttaaaatac aagggatgac tcttaatccc
     6061 actcaatcca gttgtaaacg atacgaagtg acgggcgcac agcggcagtc acatcttcgg
     6121 tactaaagtc gattgcatca ctgtagattt tgcagtccaa catttcaatt gttgtagcag
     6181 cttttgtcac agcgttaacc ccggaagatt tggattcagg ggtagcagcc atcgtgatat
     6241 caacatagtc cttcgccgca atgcgatctt taatgaactg aagaatatcg ccttcgatag
     6301 tctccacgca ctggacctgg atttccccag agtttcgaat tggaccgtgc tggttgaact
     6361 tcacaccatt cggaccatag tcctccacat cctcgcgggt catttcagga atttgcgacg
     6421 tgcgaaccag tacgctgata tcttcatggc ctgcaacagt gagctggaat tcagaagata
     6481 ccagtcgttc gcctttggcc gcgttggcag tatagcggcc cttaataaat ttacggtttc
     6541 ccttagtgtt attgtgcccc atataaaatc cttttactgg aacgcccgaa caatatcgga
     6601 gctgttatat atcgaagaac cggtcaactg gaggttgacg gtgtttttca ggaaatgccc
     6661 attgctgtcc ctgggcgcat cgagatcgaa acttatgtcc tggatagcga catcaatgat
     6721 gttgatctgg cgaccaatgt ttagcgtcac acgctccggg attcgaccac caatactggc
     6781 atctttaagt tccgggctaa tcatcgctga caatgcggcg atagctcctg aaacctccgt
     6841 gaatggatca aacaaagcga tgaaagttac tggcagcgtg aaagttggcg gtgttccccc
     6901 ctcccaaacc attaagctgt tccaacgggc caccgacgtt gtttcagtac caacctgcgc
     6961 aaaaccactg aaggcaccag caacagaccc catggacata ccggtaaacg gcgcttccca
     7021 attctgggcc atgttcattg ctgccccctg gctgatatat ccggtaacct ggtactgaga
     7081 gttcgttaaa gtaactttca gaaatggcga tacaccgtca gcctggctgt aaaccccata
     7141 aggtataggt gccattcaag ttaaaggccg gagttctccg gcctcctcct ttagccaagg
     7201 cgcttacggc gcagtttcat tgactttttg cgggcaagtt ttgccgcacc cgtctgggct
     7261 tttcgacgcg cttttttcag cgccgatttt tgagccgcag tcagacgttt tttacgcagg
     7321 cgtttacgga tgagtttgat ctcaccgtta cgaacaacct tcttaaatgc ttcagtcagc
     7381 atttcatcag aagtgccagc aacaacaaac gccgcttcca gttcgtcacg gtcgtcgcta
     7441 tctaaaccag cgatagaggc accaacatca gcagctgcgt cgtcgtcttc atcgtcagcc
     7501 agtgcttcga tcaggtcatc atctacaccg catgctgcga ggaagtcagc aacatttgcc
     7561 catgcttcgt tataggcatc gtcctgttct tctgtaactt cggagtcgtc gtcatcagag
     7621 ataccagcga tagcctgaac gaaaccatca agggagtcga aagtcagatc accgctatca
     7681 gcccaggcga aaacggcgtc ggccgcatca ctcaacgcat tctgcatagc acttctattt
     7741 gcagcttcca gaatcatctg gtgcgcctgt tcgacggtcc attctttacc gtctttccct
     7801 tccaggattt gctcaggagc cggggcagat ggaacgttat cgttagtctg tgccgccggt
     7861 tccggattat tattaataac cggatctgtt ggctgttcgg cgcttgctcg ggcagactcc
     7921 atcagctgca caggatcaga gttcaaagcg aaacgagaca gtccattccc caaaaatgcc
     7981 ccggattgaa aaaagttttt gctcattgta ttcccttact taataagcag cggtacgccc
     8041 tggatacgac gggctacgcc agtcgggcag caggcccaga ctacttccca tttatcgaat
     8101 tccgcctgcg taactttcag cacatacggt tctgtaccgt cagcatcagg atcacgagga
     8161 gccaccagag cgccggaggc gacaaagcga tctaaaagtt tggtcatccc tttagtcagg
     8221 ccagccgcag taataccgtc cgggctatgc ttcatctgtc gggctaactg gacaaagaaa
     8281 cggctgattg cattcatcag ggatgggacg tgctggaagt gcagatagtt atcctgcgtg
     8341 cagcaagtta aagcatcgtc gatgatcatc tggccagagg tgccaacaga tactttattg
     8401 agacggccct tgaccattgc ttcttcgtcc ggggtatctt ccggatacag cggttgaatt
     8461 gacgcacgag caatgacggc acgttcttca ccagccggtg agtaatgcca accgccgaca
     8521 tcagagtttt tcttgacgcc acgagctttc gccgcatacg ccacgccaga cagaccaaag
     8581 accacacggg attgggtcca tttgtctttg caggagaacg ggtagtgata gacagcacag
     8641 cttacataat cggtaccaag taaaccggta tcttcaacag cagagagcgc ttccgtatac
     8701 gtcaatgtcg gtttgacatc aaagaagcca tcaatcaggc gatcagaaca gatattacct
     8761 aacgcggtga tcgccgcatt gtcatagcaa cccaggccga gaacagcggt gtacatgtac
     8821 ggcgcattgt tcagcacctt aaccgcacgc aggtacgcag cggttgagat tttcgactga
     8881 tcaccgttgg taccaccagt gaacgccagc gattttttat ttgttacttt cgccgtcgaa
     8941 atcagctctt cattaacaac cgcgcgcaga tatttagaac gggcttccag agccgtaggc
     9001 agataacaca agcggcccat gtcatctttc gcttcttccg ccaaagacac agtgtgtgtc
     9061 tccagggtcg ttaccacgcc gagcgaagtc gtctgggtca gttttaagag gaagcgttca
     9121 ttacccgcgc tgtccgctgt tgccgtttcg atggttaact cacgggtagg tgaaatacac
     9181 ggatcaccat catcaacgta gatagcaaag gcttcgccac tatcaagttc aatttcagaa
     9241 ccgtatggca acgcactgta agccggttcg cctgattcat cgaacataat aatcgggaac
     9301 ttcgcatcat ccggaacagc acgaacaaca taacctgacg tttgctgaat agcttcgtat
     9361 acatggcgaa ttggttcgaa ctgtgagccg gaagacggct tcagcggttc gccgagaaca
     9421 tctttgtaat tggactcagt aaccgcaaga acagtaaacg gcttgccacg cgcaaatacg
     9481 ccaataccag cccacaagct gctatttaat gcaacaccgg tagataacgt cgcatcggca
     9541 ttgatcgggc taaccgcgac gccggatgca ttacctaatg actgttgaat tgaatattga
     9601 gacataactt tccctgttat gcgccccgca cgggggcgct atgttaaacg gagaacttcc
     9661 cctgattact cagagtcacc ggcatcaatc gtgtcgccgc ttatgaagtt aagcccgcct
     9721 tttttggcca ttgtcagcgt tacacgagtg aagtaatcag cgccgttgcg tgggtgcata
     9781 tcgttgatag ccgaacccca cagtgtggta cggttgacca gcgccggagt ggtcggatgc
     9841 tggaacggga tagccgggac agcatcacca gtcacgaagc ctgctttacc cggattttca
     9901 tcacggacgt agcacagcac atccatcgag ctgaactgaa tgttctctgt cgttaagttc
     9961 ttacaaatac cagcaggtac ttcgtacact ttcacgttac cgaacagggt accgatgtag
    10021 tgaacatacg gagtctggat atagtcttcg gctggctgga agaaatcctt cggcaactgt
    10081 ttgaagaaag atgctgcatc agcaccagca aacatcccca tcgcaccaga agatttaacg
    10141 cgctcaataa tgtcgcgata tacagtctgg aatttgccac gaatgatggt tgcccataca
    10201 ttaaaggact ggttaaccgg cagagcgatg tcaaaggtgt cggtcgcaag agtacgccag
    10261 atcatgatgc gaagacgcag catatcctgt tcatgagaca ggtattcctt cagggtgcgg
    10321 aactgtaggg aacccaggtc cagaccaaat tcacgctgtg cttcatacgc cgcctgtacc
    10381 gtgtgctcag ccgcgataac gaactgactt gggaacaggg tgtatttctt catttcgtgg
    10441 ttgatcagcg ggatcagctc aggtgcggct tcaatattga tttccgtctc aattgcgatc
    10501 tcagtgcctt tatccggcgc tttggagaac gacagggcaa tctgaccaat gttgtagttc
    10561 agagagcagg taacagtgat ttgctcacca gcagcattag taaacgagtg aagtaggctg
    10621 ccggaaccgt tatcaacaac agacttaata cggttaacgt agatgttagt gcgacctttt
    10681 cggattggta cattctggcc ttcgaagtct tccatcttga aggttgcggt tttgctggtg
    10741 ccatcggagc ttgccaccag cacatagcgg cgacgtaact ggctgtacac accaacggat
    10801 tgcatgtcca gaacatcacc agcagcataa gaaccaaaag aggaacctgc cacgttaaag
    10861 acttcataga tgtcggactg gtcacgcgta accggaatga aggtacacgc atcagcggta
    10921 gctgccccca actgaacagg caggatcatc gcgaggaata aaggcagacg cataacaccg
    10981 tcagaaacgc tcatcatctc tgctgcgacg gattccagca tcgctttatt agtggcatcc
    11041 atgctattgc gggtggactc aatcaggcag ttttccagcg tctggtggca ggaggccaga
    11101 atttccggac gcggcataga tttatgtgct gcggcgtagt cagccagtgc acttgcccac
    11161 gctgtagcga tttgagcggt ggcattatca gagatacccg caaaaaccgg gtctttacgt
    11221 gcagcttcaa ggatagatgc ggcacgcgcg gcatcatctt taatgaattg gttatcagta
    11281 ccgaactgcg cagtgcttgc ccagccaagc acagctttag agcgttttgc gatatctgca
    11341 atacgattct ggtattcgcg taagttactc aatttactct tccttaaaca caaggcactt
    11401 gtgtgaatcc cttttcggaa gagattttat tgaaagtcac ttgttgactt tctcgtgaca
    11461 agcaattttt ttattttttc gggagtaggg gaggaaggta aaatccaagg tgaaatcgtg
    11521 gcgatttcac cttgaaattt tagatggatt tactttaaaa acagtaggtt aatagtgaaa
    11581 tttgaatggc gaaagtttaa ggcttcggct ttttatcgag gctctttcta aggatatgcc
    11641 caatcatcct gtcgagttct tcctgtagct cttttgaaag tcgattaaac tcataagaaa
    11701 atgcacggcc tttcacgcgc ttccttgcaa agcgatcctt gtcctcaaat ttccataatt
    11761 cagtaactac ggacttatct ttagaacctt tatccgtgag tagtgaggct tcctttgtta
    11821 tcaagcgcag gattttattt ttaacttcat cttcggccat ttcttcaatg gataagatgt
    11881 cgtttatttc cggggatatg ttttgaataa gctgatcaaa ctctaaattc ttgttcccca
    11941 tttcgtcgcc aacagcacaa agcgttttgt agtccgaaaa ggttaattcc gactgcacag
    12001 ggaaaagggc gactaattct tccggagcac tcgctgcctg gagagcacgc gtgaccttcg
    12061 cctgagacag cccttctttg gctgcaatat ccttctgact catcccatca tttttcattc
    12121 gcatcaaacg cagacctatt tctcgaatgc tgtgctgcaa tgctgtctga acgtctttcg
    12181 ctaaattttg cgcttcctga acgctgatct cctggtccgt gactaaaacc cgcaacccta
    12241 cgttctctaa gatggcagaa gctcgacgcc gggaaccatc caaaatttca attttccctg
    12301 tagcccgtct aacacctatt gcagggtaaa attgctgatg cttaatagtg cttcggatac
    12361 tttttaatga ttttggcgta agagatgcct ggtcacgccc gttgttatgc tgatcaacaa
    12421 aggtatcgct ttctacctgg ttcggaggta ttacctcttc aataaatgtg gcctggcgac
    12481 cagttgataa cttgaatacc tgctcgactc gatcgccaga ggctgaagaa ctatcaaatc
    12541 cgcttaatat tgaaggatta agggttcgcc caattgttgg tctgtttttc tttgacatgg
    12601 gggtttctta ctcctcagtt agatctgata aattcaatac ggtcaaaaac tgctttagca
    12661 aaatcttccg cggcaattcg cgcgttcttc aatgcatcag cactaccaac atacgttgca
    12721 gggttagctg aaataacagt gtcaaaagac tcgccgcagc gttcaaaacc gtcaaggcga
    12781 gggaggacga catcaagcat atccccaccg aacacttctt tagccaggct atggcaatac
    12841 ttatggtctg ccttgttact caacttggac ataaaaccaa tgttagtcgc aagctggcac
    12901 tcgcagcctt catccgaaat gagtttcacc aactcaggaa ggcgggcaac gtatttaagc
    12961 gatgagtgga aatcaaccgt tgcaggcggc agaggtgtaa acagtatatt ggccgaggcc
    13021 aaagcatttt tcaggaaggc gtcaaggtga ggaccactat caacgaggat aaagtcataa
    13081 tcgctcttca gcttatcaat cacattttct ttcaggacag catggatgtt ctgacccggt
    13141 agatgctcat tgcacagctc tctccaatcg gatgcaataa aggcatcgtc aatcgacgca
    13201 ggcataacgt caaccccagg tacaacagaa ggaacaataa actcctctaa cagctcttca
    13261 cggcttacat tctgcaacat agcctgtgca gatgttgcgt ttacgatacc aatagagtgt
    13321 ttatggctta aaaacatcgt tgctgaagat tgcggatcaa ggtcaataac cagaatcctt
    13381 aaatcttcca tcagaagatg agggtgagca cgcattgcat gcgccagaga aaccgtcgat
    13441 acagtttttg acacaccgcc tttaagattg gagatgaaaa tcacatacgc ttcgctgtag
    13501 cgatcccggt attttggcac tccgcgatgt tcatatatgt caatgatgtt ctgaattgac
    13561 atcgcatatt tcattgaaga gccagcaggg cgtttatcga aaacataacc cttttcttcc
    13621 atttcactta cggcatagtc aacgttcgct cgagtcagta gaggcaattt tgccagtgcc
    13681 gctttcgcat agacctggta aaactcgttc gcgtgtagct catccttttg caactgtact
    13741 tgttcagtca gaacattgag cattctgttt gctctttgag caaccttgtg aagctggctg
    13801 gaatcactca tcgaaagtca tcctttatgc tgtatttttg aatttaatta aaaatgctgc
    13861 ataaaataat aatgtatgcg tagatgcttg tacatggcat tctctgcatg ttttgttcat
    13921 tttgcacgat tgagagttac aagaaaggca caaaaaagcc ccgttcaggg gcatcagtgt
    13981 tatttgctaa gagcagcgaa taatcgttcg aaatcgatag tatctatagc acgcgtaagc
    14041 gccggaagtt cagcctcaaa gtacccgtgt cgatcgtaaa agaagggacc gaagagcgag
    14101 gcatgttgga ttctacttcg ccccagcccg gacacacagt taagcccatt accggctaaa
    14161 aggctaaaaa acttctctgg attatcgtgg taaagctgag aatcaatggt ggcggttaac
    14221 tcttccatag ggaagcacac ccgccctgtc tcccagggat atttagtccg aagcataaac
    14281 attgctttca gcaattcaca ttgagcgcgg atcgcgtccg gttcatagcc agatatggag
    14341 acataagcca cgtccctcat tcctgcgtca tctttgaaag tcacgataga agtaacatcc
    14401 agttcttttt cgaaagagcg agcagcatct actggacgct gaagtaaatc attctactta
    14461 atgcgctcga gaatccctcc ccacatatca tttagatatt cgatatgagc caaaacctta
    14521 tcaagacact ctcgtgtaaa ccattcagta tgcccgccac cggcgctttt ctcccacggc
    14581 gcattccagg ggaaaaaggt tgcgtgtaaa gcccgctcaa gattaaccat tgccaaacgc
    14641 gtaccacgat agacccgtga aagcgcaaaa tcgggactca cttgtagccc tttaaaccgt
    14701 gccaatggac cacatgaaat gccgatttta aaagtatctc cgttctccgg caccagaacg
    14761 tagaggtagt gttgtttctc ttcttgcata tcaataccac tgcttgatga gaaccgcgca
    14821 aatgttgact atgcgcgatg tgactacagt caaaagttga ctgtagtcga tttaactcca
    14881 ccaaagatcg actatgtaag atattgtcgg gagaaacgtt gactatacgc gatgaaatga
    14941 ccctaaaagc catctcaata gcgacttgca gaatattgac gccagcaaaa atccaccagc
    15001 gtcaacgaat gtcgcctata gtcaacttct cgctatcgca tatagtcaat attatggatt
    15061 gcgcttatgg atctgaaagc cgattttcct gccgtttttt atctctgaaa atttaagata
    15121 ttcaatagct tccaaatctt tcatggcttt tctgataacg ctattttgca cgctaacgga
    15181 tgatttgaga ttaagccttg ctctaaggcg ctcaatgctg acaggtgccg ggttggcggg
    15241 tagagcctca aagaatgtat acagtacctt ggccgtctct ttgcgcccga gcttatccag
    15301 catcttcagc ttcaggattc gcttatagtc aacatagtaa agttcagata gctgtttctg
    15361 cggctggatc tcgataacat caagctcggt attcaggctg ctatatgcca acaagttgac
    15421 gttaatgtta ttgagatgac cttttgccgc cgggaagcgg aatttgacaa ctgtctgctg
    15481 aatgcgtgtc agagagtcat caatactttt acggaacgcc tttgaaaggc gcttacgtgg
    15541 atagccgcat cgatcggcaa actcggagaa tggcagggtg attatgccgt catcatcagg
    15601 tgcgtagtca aacaacgcgg aggttatgcc cacccacacc ttaaaatcag tatccatatc
    15661 caggcgtgga ccatgaattt caattccctc atagccttcc tgctcaacaa ttttgaggct
    15721 tgatagttct tcggttgcgt tcgttgtgtt tgtagtaact gacgatccgc gacgtagtgc
    15781 cacattggta gattttaagg ttggcacaaa cacacctaag cgcaacaaag caatgggttg
    15841 tatagtgcta ttgttattgg gtttcaggct gtggatttct cctgtatttc ctgcaacttc
    15901 ttcaacgcta aggaagcctt tattttcttc cagcatcgtg gtttctccat atgtggcgcg
    15961 gcctggcgtc atctggattg cagttatcaa caactgtgaa tagtcaggct ctaaaatcgc
    16021 gtacagtcaa tgtttctgtc gcatatagtc aacaataaat cgcgtgcagt caacaataaa
    16081 tcgtgcacag tcaacataaa atcgcgtata gtcaatgttg atcccatttc aggccagaaa
    16141 tggcgcggct tacagcgatc cgggatcttc tttggatctt cctgggttct ctttaggatc
    16201 tgtttattgg atctatgctg tggataagtt gaataaaccg gccaacatag ccggttggaa
    16261 ggaagggtat tattctacgc tttcgataag aagaccatgt tcataacatt taagctcatc
    16321 gccttcgtac aggaattggt atccaatacc accattttca tggacattag ggaataactc
    16381 ataactcact gaagagcaaa tcacaccaat gcagcgatca acgccttctc gttcttcagt
    16441 gctgaaaaaa tcctcttcgg taagaacatg agtacattgc tcatcagcat aggtcggaaa
    16501 tacatgctcg atgcaatccg ggtgttttaa accaagctga tcggcaagct cgaaagcatg
    16561 acggtattgt tcagatcctg gcttgccaac agtgatgtgc tcaattttgt aaattgaagt
    16621 cgctttgttg atagtttgct ttactgttac tttatcagac ataaaaatcc cttttagtta
    16681 ccgctgatag cgcggttgta atcattaacg ttgcgattct tcctgttaat ccccatcagc
    16741 atcgtttctg tatcgaggat atacgctggc agatcatcaa aatattcact gctaaactct
    16801 ggcatcctgc acataaacgc acttttgggg gcagggtggt taacctttgt cggcgtcggc
    16861 gttaaattcg ctgatcgact cccggagcaa ccgctgagtg tcagcaggaa tgcgctggcg
    16921 aacattaccc gccgcaacca gttgtttctg aacttcagct tttcgttcca tttgcctgtc
    16981 agcatatttg gcttgttctg attcattttt cacttcctgg ctgtgaaaat gttgctctgc
    17041 tttgttcatc gtctcaatgg tctggttaag atccattatt gacttatcac gttccttaac
    17101 agcctgatca agactgccaa ttttctccat ggcttgcttt agctgatgac gttcccatgc
    17161 aaacccagca ccaacaagtg cgcaaatcag aacaagaaca ccagtagcag caagtttctc
    17221 cttcaaagac aaagctgttt ttaacgtaga aaagaatgac atgtcttcct cctgaagaaa
    17281 aattatcaat gaagtccttt gttaccgtgc cgctttgttt aattcatcaa gaacagaatc
    17341 aggaaccaaa gcggcgactg cgctggctgt gctggcctta tttgctgatg cttccgcaag
    17401 cgcggtaccg atagcatggt tataagcagt tatggctacg ttggcgcttt cattcgctcg
    17461 ttcatactgc tgttgtaacg cagttgtggg tgctgttgtc tggttgaaaa caaccccaaa
    17521 ctgttcagtt gctactttca gagattcaat ttgctcttct gttagtgctg gtgggggagt
    17581 ggcagtgccg ccgcctgaac cagagcctga cgagctttct gagccagtgt taagggcctg
    17641 gttaatctcc cccattgcag cgactaaact tgatgtatta agcgcgttaa cagcgtcctc
    17701 aagcgattta gtaatattca catcaccaat ggcaatagag atcggcagtt ctgaaacttc
    17761 tcgctcatta gcacggcagt aaacatccca accaatatcg agttgaagca gcattgacag
    17821 atctgcataa ccagccaaca ggtccgcgtg cttagttgcc agttctccaa tgttcgttaa
    17881 gccggttgtg gttgttctga tcgttgaaac atagctggta atagtgtcgg gatagacaat
    17941 tgtatccaga attaatccgg tcaattcttc tgcaagcagt tttgctgtgt tagcactgtt
    18001 tcgtgccgat gttatggcac caggtgtttt catcccaccg gcggcggcca attttttata
    18061 tgcggataac tggtagtctt tttccagcat gatatctcct aacttacctg aaccaggccg
    18121 tctccggacg ctacggtaga tccgcatgaa acagggtcac caacgcatac gatcccttta
    18181 ccattgacgg taaaccatgc cctggttgat atagcttgcc caccgtgcgt actgtttcca
    18241 tcggtatgct gtgcatattg cttaccatca actaacactt cgactccgtt gactttaagt
    18301 agtggttcac tctctacagg aggcctggat gggaatcctc cgtgccccga acaaatgctg
    18361 tcttttgttg caatgcttgc cacgccatca ccaattattt gctctgattt tcgttatttt
    18421 aacttaggtt atttgtggtc tgtatggcgt ttacttatcg caaaattgct ctaataaata
    18481 ttgtttttta tgtcgtattt tcggtaccat tcagccatcg cccttcaatg ggcatttgtt
    18541 tggagtcgtc agatgcagat ggagctaata agccgcaaag agttcgatag ccgtgtaacc
    18601 agcggtgaac tcgacaactt gcaggctatc aaggtgaaag aaggcttttg cctcattggg
    18661 aatcagagcg gaataaatcg cgtttttatg cttcgccgta cggatttgaa gccatttgtc
    18721 tggaagaacg aaattggtcc cagctcatac gctcaaacga gggggtgcca caacctggcc
    18781 tttttctaca aagacgagct ttctgtggtt gatattcaag ggttacaaca tgtttaagca
    18841 ctggaaaaac attactattt ataaactttc tcgtgaggcg gatctgaccg acttagaaga
    18901 taaaaagaaa atgatccttt tcacgccatg cggtagtcag gatatggcca agttcggttt
    18961 tgtatcgcca tttggtgata attctgaagt tatcgctatg catggaaatg gttttatcct
    19021 tgttgaagca aagcgcgaaa caaaaattct tcccccgccg gttatccagc gagctattca
    19081 agaaaaaatt gaaaaacttg agcaagaaca agcgcgtaaa ctgaagaaaa cagagaagga
    19141 ctccctgaaa gacgaagttc tgcattctct tctgccacgg gctttttcaa agttttctgt
    19201 tatccaggcg atctacgacg gttcaactaa acgtatctat atcaatgcca gcgcgcggca
    19261 ggcagaggat atgctcgcgc ttatgcgtaa gtctctgggt tctcttcctg ctgttcccct
    19321 gagtgttgaa aatcccattg aattaacgct gaccgactgg gtacgtgatg gtagtgctcc
    19381 acagggattt caaatggggg atgcggcaga acttaaggca gtgcttgagg atggcggtat
    19441 tgcccgagtg aaaaagcagg atttgggaag cgatgaaatt tccacacacc tggaagctgg
    19501 caagctcgtc actaagttgg cactcgactg gcagaaccgc attaaattta cactggacca
    19561 taacttcagc cttaccagcg tcaaatttgc ggatgaattg cttgagcaga actctgatat
    19621 tgatagtgaa gatgttgcgc agcgactgga cgcagatttc ttcctgttga ccagtgaaat
    19681 ttcgtgcctg gttgatgctc tggtaaatgc ccttggcgga gaggctaagc agtgaaagag
    19741 ctgtgctatg gatctgtttg cagtggaatt gaagccgcga gtattgcctg ggaaccgttg
    19801 ggtatgcgtc cggcgtggtt tgctgaaatc gagccttttc catcggccgt tcttgcgctc
    19861 cgctggcccc atgttgccaa ccttggcgac atgaaaaaac ttgccaaaaa agtcttggct
    19921 ggggaaatcg aatcccctga cgtgctcgtc gggggaacac catgttttac cgcggggcat
    19981 atggttcttt gtaaaaatgg ctataaacca atagaaaatg tttgccctgg tgattacgta
    20041 gtcagtcatc tcgggcggtt acaacaagta aaaagagttg gttcaaaaat agctaatacg
    20101 gggttactta atgccgttgg gcagccttta ggtataagaa caaccaatga ccatcccttc
    20161 ctggctgttc ggtggaaagc ccaaaacacc cggaaaaatg gcacatattt taagagagag
    20221 ttgttgtctg aaccggaatg gcgagcagca tgtgatatgc cgggatatca atggtgcgct
    20281 ctaactaatt tcaatattgc atttccagat atttgttctc ggttcttgtc tgaagaacag
    20341 gctatgtatc ttgcgggcgc ttatgttggc gatggatata ttaggagatg gagaggtaaa
    20401 tctaagaagg cggttgtttt tggcataaat tgccagaaat tgagaaagtt tcattgccgc
    20461 ataccagaaa acatattttc cgtggcaagc gaaatccgag ggagcatcaa agtaaccttg
    20521 aatgatacgt gttatgccaa ttggcttaat gaacattttg gcgagttaag ccatgctaag
    20581 cgtatccctg catgggtgat gtcgcatcca ttgcgtcatg tgtttttaca aggctatctt
    20641 gatactgatg ggacaccaag tggtaaagcg ggatttagaa ttaatagtgt tagttctgcg
    20701 cttgcttggg gcgttgcggg gttgtcacag acttgtggtt atgtttcttc ggtcagcttt
    20761 attgaagttg agcccaaaaa agtgatcgag gatcgcgtgg taaatcaacg gaattattat
    20821 caggtaacaa tctgcccgca gaaattgtca cgtaaatcaa gattggctca tggaatgctt
    20881 ttacgaacag tcaaagagtt taaatcggta ggcctagata ctgtatacaa catagaagtc
    20941 gaaggtgatc attcctatat cctcaatggt gcggtggtcc ataactgtca ggcattcagt
    21001 atcgcgggat tacgtggtgg gcttgatgat gagcgtggcg cgctaacttt aaagtatgtg
    21061 gagcttgcaa atgcaattga cgacaaacgg gctgagtcat ttctcaaacc agccgttatc
    21121 gtctgggaaa atgtcccagg agtcttgtca tcggcagata acgccttcgg atgtttcctt
    21181 gccggattgg ctggagaaga tgtgccattt gaaccaggtg atcgacctga atcaggaaaa
    21241 agcaacgcgt tctggcggtg ggatggcaaa accggttgcc atgttccaaa gtggccgcag
    21301 tgtggttgta tttatggacc gcagcgaaaa gtggcctgga gaatccttga tgcccaatac
    21361 ttcggagtgg cacaacgacg ccgacgcgtg tttgttgtcg caagtgctcg aacagatctc
    21421 gatcccgcaa cggtactttt tgagttcgaa ggcgtgcgcc ggaatattgc gccgagcaga
    21481 ggcgagggga aggaaactac cagatatact tcaaacatcg ctatcagaac ttgcgatgat
    21541 acaaacataa ttgccatggc acatgggcaa ggaggggctg agataaaaac cgataattcg
    21601 gcaccaactt tgacatgtaa ccatgaagca ccaattgtat tgctcggcga cggtagaatg
    21661 cgccgtctta cccctgtcga atgtgaaagg ctgcaaggtt ttcctgattg gcatacgttg
    21721 atccctacgg aaaagcgtaa aaaagttaat tcagatgaac tggcatatct gcacaatcac
    21781 tatccagatt taagcgaaga agaggccgca atgcttgcag ctgacggacc gtgttacaaa
    21841 gcgatcggca atagtatggc aataccagta atgcgctgga ttggcgatcg gattactaag
    21901 gccgtatgtc ggcagaaaga aggaagtgaa acaaaagagc gaaaagttaa accagcggca
    21961 gaattcgaac ggtccatatt caaatgggct ggtggaaaat ttggtgttct ggaacaaatc
    22021 tttcgctatt tgccagaagg gaagcgcctg attgaacctt tcgttggtgg cggagctgtc
    22081 ttcatgaatg ccggatacca ggaaaatctg ctaaatgatg tgaatgctga cctgattaac
    22141 ttttacaaga ctctgcaacg cgaggcgcat tcacttatca ctctggcaca tcgtttcttc
    22201 caggactaca acacacagga aggatacctg gcagtacgga atgcgtttaa caaacaagtc
    22261 tatgatgatt tacatcgcgc agcggcgttt ttgttcctga accgacattg ttttaacgga
    22321 ttgacgcgtt acaaccaggc cggtgagttc aatgtcggtt atgggaagta taaaactccg
    22381 tatttcccat tacaggagat ggaagccttc ctcggtgcgg aagggcggtc tgagtttgta
    22441 tgcggtgatt ttgcagcggt gattgaagct gccggagaag gagatgtcat cttttgcgat
    22501 ccgccgtatg aaccgcttcc aaatacagag ggattcacga actattccgg tcatgacttt
    22561 aagtttgaag agcaaaaacg cctggtgtct ctgttgacgg atgctcatcg tcgaggtgca
    22621 aaggttctca ttactaacag tggcgcgcca aacatcagag agctttatca tgacagtggc
    22681 ttcagagtgg aacctctttt tgccagacgt tctgtgtctt gtaaggggga cactcgaggt
    22741 gtagctcatg acgttttggg tatattgctc taataaattt attagtgtaa tattacctca
    22801 atgaatcgtg atttatagag cgatttagct gttagccgcg acaggcgcgg cggtaagcat
    22861 ggctgggcct agtcctccca gacaaaccac cgagttgcca ggttgaccat gcgcctaagt
    22921 ggcaacgccg aagtgcgtta cgagcgtcca gtttgcccat cttcgggtgg gcgttttttt
    22981 cagggttttc gtcatggtta gcgactttgc ggcggtttag aaactgacca ttaaagtaaa
    23041 tgcaaacgat gatctgatga tggtagcggc ctaagaagcc agatgccatg gggtatgagt
    23101 cgtcccccgt caaaaaatcg accgcagagt gtccccgtct gtgtattagg gaacggggag
    23161 gcacaacagg taagggcgct ggtgtgatta accagatgaa cgagaagggg ccatctgttg
    23221 gtcagcgtcc tttcctgttg cgttttcttt tcagcgtaac agcggtgctt aacagcactt
    23281 tgggtacagt tccacgaatt tacgggtata tcccgtcatg ctgaaggcgc taatcacgct
    23341 ggaagccagg gttatgcatc ccctgttacc gaattgcagc cagggcgcgg tgcgccgaaa
    23401 agcatacgga ggtggaagcc ctcgccggag acgtacccgg caagtgatgg tgtagctcag
    23461 cggtagagca gttggctgtt aaccaactgg tcggtagttc gaatccaccc accatcgcca
    23521 ctttagggga gttagtccgt aggggtagcg gggtagactg taaatctact gtcattgcga
    23581 ctcgggtggt tcgactccat cactccccac cacattgccg atttagctca gttggtagag
    23641 cagtcgcttt gtaagcgaat ggtcagcggt tcgatcccgt taatcggcac caacataaca
    23701 ggtaagagca ttctccctta tggggcctgg cttaaatgca tcgagtgctc ttaccgttgt
    23761 gatgaagtgc agatcttaga agcaaccaga agataagcat ctggcttcac aacataaacc
    23821 gcaggaacga ccaataaacg gtagtccgta tggagaacac cccgttgagg aagaggcctg
    23881 gccggaaccg taaccggcga ctggtaatgt gtgtaaatgg agcgaaatat gtgggcgttt
    23941 aacgggatac gggttgtaac agcgattatt aacggcgaac ttaagtattc acgtttcgag
    24001 acaaatgaac gtgatgggaa atgtattggt gtgcggttag taagagaata cgcataacaa
    24061 ctacaacgtt gagaacactg gcgtaacggg gtcatatccc aatctacgaa taaatgttgc
    24121 gttgccgcgt gacaaccagt gttctcaaca ttgtggtgaa tgcacaggct gatgtgccgc
    24181 aactacagta gtgcgcgctt tgcggggctt gctacaaccc tgtgtcggag ttcagcaccg
    24241 accatcacag tttgattctc tggcatgagc ataacgctga aataagtcca gtctggtgcg
    24301 gtctgatcac ccgccgttag ctccacgaaa cggagcacgt aacaggtaag agcattctcc
    24361 tgtaacgggt tcatatccca atctacaggt ccaccaagaa tgctctttcc gttgcggtga
    24421 atgcggctaa gcgcacgcgg ggaaatggtt atatctgtcc attatttctc cttgtttcca
    24481 cgtccacggt ggataaccag ccaaaggaca ccgggaggaa cccggcaccg cagctttttt
    24541 attcgttaaa taatggagtg agaggatgca gaacaatccg aacaaatgtc gaacgctatg
    24601 ggtgcggtta tatatttatg ccgtcctctg tttgattgtg tcactggttc tgtatgtttg
    24661 gcttttgcca aatatgatct catctaacag cacaatactt gtattgttgg gagtccttct
    24721 cgcgctcatt tacccggctt tcgcagtagt cttttttcgt gaaaaaacca ggaaattaat
    24781 taatgaaaaa aacgttgatt agtgcagcga ttattttggg ttctttatgt ctgaccggat
    24841 gcgatcgggt agagccaggt aacgtaggga tcaaagtaaa taagctgggg gatgataaag
    24901 gtatcggtga agtagttggc gttggtcgct actggacagg cttgaatact gaagtttata
    24961 tcttcccgac ctttaagcaa atgaagacat acgatgagcc gttcagcttc cagatgagcg
    25021 acggaacaac cattggttac cacatcggcg tagcctacaa ggttgatcca gcaaaagtca
    25081 caacggtatt tcagacctat cgcaaaggtg tagatgatat tactgatacc gatctacgcc
    25141 agaaggttgc agatgctctg aaccgattag ccagcaaaat gaccaccgac aaatttatcg
    25201 acggtggcaa atctgaattg cttgatgcag cccttaaaga cattcaggaa gaaatgacgc
    25261 caatcggcat tcaggtgatg agcctctctt atgtaggtaa gccagaatac ccgccaacag
    25321 ttattgacag tattaacgcc aaagtcacgg caaaccaaaa aaccctgcaa cgcgagcaag
    25381 aggtcaaaca acgtgaagca gaggccaaca tgctgcgcgc ggaagctgcc ggacaggctg
    25441 atgctattcg cacaaaagcc caggccgaag ctgatgccat tcgtttacgt ggtgaagctc
    25501 tgcgccagaa ccccggtgtt atggacttgg aagcgatcaa caaatggaac ggtacattac
    25561 cgcagtatat gaccagtaat accgctgttc cgtttgttcc ggtgaaataa aagcgtaagc
    25621 aaaattggca gtaatccggc cctttagctc agtggttaga gctggcgact cataatcgca
    25681 cggtcaccgg ttcaagtccg gtaggggcca ccatatttgg ttgtaacacg gcgtctggca
    25741 catgcgtcgt tagcggtctg gtgacgttaa aggggtaacc tttcccctag ctcaggcaac
    25801 aaaccaggta gccggaatgt gcaagccccg ttcatagcgt cggactgcgg attcaccatc
    25861 ctggcgattc ggtgtgacag ccgggaagag tccggcgcat taatcctgat tttctggtga
    25921 tgactcatat cgttaggagt gatttgagta tgccgattat atctgacatt cagcacgcct
    25981 gggtggagtg ctaatgtctg catcccctct tgaatccatg ccaaattccc ttagtgcaga
    26041 acaagctgta cttggtggct taatgcttga taactgccgc tgggatgaag ttgcagatcg
    26101 tatagttgct gatgattttt ataccagtgc tcatcgtgaa attttcagtg agatggagag
    26161 gttattaagt catggcaaac cgattgattt gataacactt gctgaagcac ttgaacagaa
    26221 cggtaaatta gaacgcgccg gtggttttgc gtaccttgcg gagatgtcaa agaacacgcc
    26281 cagcgcggca aatatttgtg cttatgcgga tatcgttcgt gaacgcgcgg ttgttcgtga
    26341 aatgatttcc gtcgcaaatg aaatagctga agctggatat gcgcaggatg gcaggggcag
    26401 caatgaattg ctggatatgg ccgagcgccg cgtttttgaa atagctgaaa aacgacaaaa
    26461 gagcggtagt ggtccaaaag atatcgccag cattctcgat gcaacggtat ctcgcataga
    26521 agagttgttt cagcgaccgc atgatggtgt aacggggctt gataccggat ttaccgatct
    26581 caataagaag acggcaggac ttcaggcgtc cgatctcatt attgtcgccg cccgcccatc
    26641 gatggggaag actacgtttg cgatgaatct cgtcgaaaat gccgcagtcc gtaacgataa
    26701 gcccgtattg gtttttagcc ttgagatgcc gagccaccaa ctgatgatgc gctcactggc
    26761 ttctcttgca cgcgttgatc agactcgtat tcgaacaggg caacttaacg acgaggattg
    26821 ggcgcgggtt tctggcgcaa tggggattct gttggacaag cagaatattt ttattgatga
    26881 ctcaagcgcc ctaacaccta cagagcttcg ttcccgcgct cgtcgtgttt ataaagaaaa
    26941 tggtggtttg agcatgatta tgatcgacta cctgcaactt atgcgcgtcc ccgagctgca
    27001 agataaccga acgctggaaa ttgccgagat ttctcgctca ctgaaggctt tggcgaagga
    27061 attacaagta ccggtggtgg cattgtcaca acttaatcga tcgcttgaac agcgtgcgga
    27121 caaacgaccg gtaaattcag atttacgtga atcaggagca attgagcagg acgcagacct
    27181 gatcatgttt ctgtatcgcg acgaagttta tcacccggat agcgaaatga agggcattgc
    27241 cgaggtaatt atcggaaagc aacgaaatgg cccaattggc acggtgagat tggcttttaa
    27301 cggccaatac tcacggtttg ataactatgc tggtgctgac tggcaagagg attattaatg
    27361 caatggaatg aggaaaagcc gatgaacatc ctgatcattg ggcgaaaatt tgaagctatc
    27421 agtgatgtga aaacatatac ggaaatgtgg gcttacaacc tggcctgcgc ctttagtgag
    27481 gcaggggtaa cattgcaatt ccatcgtcca tattcccccg gcgtcgaaag cccggaggat
    27541 tatgttgaag ctgtgttgac cgctgcgacc tcgtgttctg cgaaagccat tttagcgcca
    27601 ggattgcggt attttactac ggtgcccagg gaaataggcg tgcaactgcg tcgtcgattc
    27661 actggatggg tagcccaggt atacgacggt tctatgctgg attcggcacc agtcgatatt
    27721 acttttactg tccgcgatga tacctggcgg tacctggata atcccggcag gttagagcgt
    27781 cataatcgct ttaacaaaca tgttggatgg gcagcgaatc aggatctgtt ccatctggaa
    27841 accaaaacag acgatgttct gcgtattttt gtagaccacg ctgcatttga tgttagtggg
    27901 tttgatcact ccttaagtat ccttatgaac cttcagcgtc tgaccgttcc gtatgaggcc
    27961 agaacgttga ccgatgacgg attgattacc attgatccgg ggaatatatc ggtaacccca
    28021 tacagacgga cgccggtacc agcaaccgaa tttgcagctg aattgcgtaa gagtgacgtg
    28081 tttatcgtta cgcatcccga aagccttgga ttaactgtac ttgaggcggc aatgtgcggg
    28141 gcgttggtat taacgcctcc cgattgcctt ccgccagatc gcctggcttt ggtgaaccat
    28201 atggttatca agtcgcggat tgattgggat gaggttattg ctcgcgttga tcgcgtgaaa
    28261 aatgctgaaa aggtccagtg tcacacctgg tcggcaattg cggaaaagat gcttgagacg
    28321 tttatcacgc agaaaccgtc gcgcggtaac ggataaaaaa ttgaacccgt cataacagaa
    28381 aagcccgaac gccgggcttt tcttaagcct tgtcaacaga gacttgagcg gcttttatgg
    28441 atagattccc gctggcctct atcgccatac ttccccccgc cttcagggcg acatccgcgc
    28501 ctgactttat atcgagattt cctgcggaag agatgaatgc cggaccttga gaaatggcat
    28561 ataactcccc ggcctcgttg aaccctattg ttgttccact tttcaagtgc gtaacggccc
    28621 aggctccgcc cgccgtccgg acctccatta gtccgttccg cgacgaaata aagtcttttt
    28681 tggcgctggt tgatggttgt gctggtgcac cttcgacttc aggcggtaca tagccttcac
    28741 cttgtcctga cgcttcaggc ggcacattgg gagcgccacc ggatgcatcc tgtgcataac
    28801 cgattatcaa tggccatcga gaatccccat tgtagggaaa ttctacccat actttatcgc
    28861 cgggcagaaa tggtgaaaac gtgtttgcat tggacaatat agcttctgcc cacggcaatg
    28921 aggcatctgg taacccatcc atcatgccga caacgcgtat ttgtgtacgc atcagacctt
    28981 tagggtcatc gacgcttacc actacagccc gatacttccc tgtcaaacta cccattcacc
    29041 actcctaact gtgctcggct gacaaaacgg aagcggtctt cgaaatgagt cacggacatc
    29101 actatcattt tgtcagggat agattcatcg agttctccgt cacctgccgt gttatgcacg
    29161 acaattttca gcgtcgtacc cggagttagc gcggcatttc cttccaccag catatcgagg
    29221 cgggggagaa tgaatttgtt gtagttcgcc agcgcggtag gatcgggatt gctcgtaaat
    29281 ttaatggggt cttcctggtt acctgagtaa accacacctt tggtcatgtc ataactggcc
    29341 attctgtaat tgtggcggcg ctggtattca taatcggcat tcaggatgtt gaactgacta
    29401 attgtaaatc cggatgtgtt gggattggcg gactcataag taagcgatgg agcggcgttt
    29461 gccatttttt ccatactttt aaaattgatc gtccccctgg atgcccagca catagaaccg
    29521 gtatcgcggg ctatctcctg caataccttg gtcggttttt ctccaacatt taggtgatat
    29581 gtggatgttt ttctgaatga gtcagcattt accttcagac caggggcaag agaggaaact
    29641 acggctgatg ggggcttatc aacaaaatac tgtgcgctgg tggacggaac ttttaataac
    29701 cgcaccgggt tactaaacgc gtaaatcagt acagtatcgt ccttgcgcgg cgctttaaga
    29761 acgaagaact cttccgagaa caggatgccg ccatgacctt ccggatcacc aagtgaaaca
    29821 gtcagtattg ttccaaattt cacccccagc ttattgacca cgtaagccgt tgaatccctg
    29881 accatgagca taagctgggg accagatagc tccccgggtt cgacataggt acatcctacg
    29941 atcatttcgc gagggatttc gttctgccca attgaaacag attgcaggaa tagctgagtg
    30001 cgttttgaat cagtttccgg ggctgtggtg gtctttgtgg ccatctcatt cctccagaat
    30061 tttcgctttt accgttatgg tgccggtggt ttgctgcata taagccagga taggaagctc
    30121 cgccacaacg gtgaggttca atccaaccgc gaacagcctg ttgtcggcgg tgccggtggt
    30181 cagatcctga aatgcgattg atttttgccc ttctatgtaa caggtaaccg gtatctcata
    30241 accgccgaca ttggcaatgt gagtgaaaga tgcctgcccg aggctggcat acattcgtag
    30301 ccagaatgct aatgcagttg taaccatccc aagagattcc ttctcgtcac tggctatcca
    30361 tagcgaatat tccagtgaga aagggatagt cgataccagg gcttcaatct catcattttc
    30421 attggtgaca tgcccttcat cgtaattatc ccggcacagt tcaccttcat aaattgaaaa
    30481 cgcgggagaa cgagacagat tcacaagcgg cattgccagc ttatttaccg ggccagcaga
    30541 ggcagtatct ttacgcccgg cgcgatcggc ttcaaatgac gacaaccact tcttcacatc
    30601 actaaaagtg ccgagcgtta tgcgatctct tggggtgcgt ttcaggaact ccctgaatga
    30661 ctggttagtg cgatcattaa agctgacaac ttgtgagtcg aacgcttcgt ttaaagcctg
    30721 tgcgagcgcc gaatcaatgc catcaatagt ggcaaattcc agcttaccgg ttggagtaag
    30781 accttttttc ttaaagatgg ccagtagcca ttcctgatta ttcagaatca ccgatgaaat
    30841 tcccttcaaa ggcgcgtgaa ggcacgcaat aaaacaaact gcctaccctg gcagtgccgt
    30901 aattgaatat tttatggatg taccagaagc ggcgaatggt tgtgccgtct gacagctgtt
    30961 ccagccattc gagcatagaa cccactggca cattgacggc ggctaaccga aggattaaag
    31021 cactgtcgct aattcccgta ttatcactgc cgtcgtatag cgcgtagaag gcgtccatct
    31081 catccgggca gtcgagggcc gttatcagtt ctggatcctg atagtcatat atgcgttggt
    31141 tcggttctat tatttcaggt gccgtttcag gtgcattttt gtttctgtaa ggtattgcgc
    31201 gatacagaac tgcatcgaat gagtcagggt ctagcttgat tgctttgagc cagtccatcc
    31261 gcacaaggtt attaaaaact gcatgacctt gataacggtg gcgcacacca gaatcactaa
    31321 gcaggccgtg atccagattg ggaaggtgat tgtcctccac aggatcaaca atattaccaa
    31381 cgttaacacc atcggtttcg atttcagcat caatatcatc ctcttcaatc agttcagaac
    31441 cttcgcctgg aatatccgga tccgattcgg tgtccgggag gttatcacca gtcacttgtt
    31501 gtgatggttc tgtgtcctca aacatgtcat caaagaaacc agccatcgat tatcctttcc
    31561 gtttacgggc ttcattaatt tgtgtctcaa gaatgcttcg cgcctgcgcg gtggcagcgg
    31621 ccttgtccat tccctgactc atgaaaaact ttatgaggtt gttcgcctgc gtttgcaggg
    31681 cttttttgag agcgtcggct tcagcgcgag cctgggcttc cctcacccgc gatgctttta
    31741 gttcggcatt cttcctgttt gccgtggtgc gagctttttt taacaaccgg cgaacgttgt
    31801 ccgtggcgct atctttggcg cgtagttttt tgcctaatgc atcctgagat ttcagataca
    31861 gctcatactc acgcgcagct ttagcctgat ccgtcgttgt tgtccggttg cgcgcgagcg
    31921 atttagccag ttcgcctttg aaataggttg ttgtcttccg cttgtcatcg ccgaaggcta
    31981 cctgttcagc tgctttttcc agggcaataa tgatggcctt gtgccatgtg ggagactgaa
    32041 aacgcgtcat agcgtgcaac acatgtttgc aggctacacc agtcagatca gggttgcgga
    32101 tcttggggaa ggcatactct tttggcggcg cgacagcata gttaccagca gtggccatat
    32161 aacgatacca gtattgatgg cgtccacaat cacagtcgaa agatacccgg cccttgcaga
    32221 tatcggcagc gattcgggct tttttcgcac cgtcttcagc aatatcctca acggctttat
    32281 cccattcctc aaatcgaatt ctgacacggt gatgctggtg gaccgactca tccgaggcat
    32341 taacagatat caatgcaagg ttgtgtttta gcccgaggaa tgtcgcggct ttgatccctg
    32401 tgccatcaga aaccttgttg ttagcgcgtt ttatatcaat gctggtggac tgcgccacca
    32461 gctgagcata ggtaatgccg ggtaccgtgc tcttgaattt ggttttatga gcctgccttg
    32521 aggtgttgaa actgcgtata tcttcgggcg taaagtaggt gccatctttc tttttcccaa
    32581 ggctgaggaa tgcctcaagt tcgcggttac gcatccccat aatccttggg gtgagtgtac
    32641 gtcgcgcgtt tcgccgattc tgacgctgct gtttacggat aagatcgaag accttgttaa
    32701 agtcttttgc acttaatcca tcagtctgat agcgaccaag gttgtcgcga gcatattcag
    32761 ttggcattca tttcccttac gcaatggata atgtccctat tacctggccg tcgtattgga
    32821 aatggcgaat catttcgcgg atccaggtgg caggtgggag ttttaatttt ttgccaacag
    32881 tcataccctg agactcatcc tcaagcccgg cggcgagcgt cacaacccag cgtagctctg
    32941 ctatgcccca catacggtaa gccagcaaat ccgggcgata ttgctcatcg ggaagaacgt
    33001 aataaatcgt cagattcttg tcgttcgatt cacacataag catcacctct ttgcgtagct
    33061 ctgccctgag tattggatcg gctatgttgc ggtcgtcata ccgcgacaga ggatattgcc
    33121 gggtgctttg ggttgtagtg attgatgtag ccatagtcag cctgccagaa atagatgatg
    33181 gtgattctac cgctagtcat ttgttgatta tttaactcaa taaaataaaa ttattagtgc
    33241 aattttgatt gtgaaatgta tcattctgcc cttaagtagg ttcttcacga ggaaacaaaa
    33301 ttggcagaac gtgttgatga tgcagagctg agcatgaatc agttagaagc tctcaaagac
    33361 atggccatcg ataacatcag aaagcaggca caggtcgtga gccaggtatt tacagggaag
    33421 tgtcgttact gcaatgaatc gattgaatca ggcatttatt gtgacgctga atgtgcgcaa
    33481 tggcacaggg aagagcaggc cgcaaaacag cgtaaatatg gcatgcgacc ggcaggattt
    33541 gactgattat gttgcgcttt actgaggaag agtttcaggc ttttagtgag cgtcgaaata
    33601 aggggcggtc caggccaaaa accaaaaagg atccattctt atcgcttgcg ccggtaaaag
    33661 aagtttctcc acatgcgaag gcacttgcag cacttgcaaa gaacccagac ctgcgcgacg
    33721 gaaattgcga gcacttcgag caggttttca tttttgatta cttcgaacgc aagcaccctg
    33781 acatctatga gctgttgcat gcaacgccta acggagggaa acgttcaaaa gcaaccgccg
    33841 ggaaaatgaa ggctgaaggg cagaaaaaag gttatccgga catgagtctc gataaagcat
    33901 gcggtattta tcacggcatg cgaattgagc ttaaagaacc aaatggtaaa gccccgacga
    33961 aagagcagat cgcctggatg cgcaggctta gagaggaagg ttactacgtc gttcttgcgt
    34021 atggtgcaga acaagcgata accgccatcc tggaatacat aagccttaaa aagggtgagg
    34081 ctattgagca tgtattgaac ggcgacaagt ggttgtatgc tgcttaaaat aataaattaa
    34141 ttagtgcatg tacgctcttt gtggtagtgc actttaacat cgggagaata atcgtgtcat
    34201 ccaaggttaa ttatgagtcg ctggcatcgg tcatgccgcg taatgaacag gaaacagatg
    34261 ctgtagtgga ccctgtaatc gctgaaatga atgctcgcct ggaggctgaa tttgcagctg
    34321 agaatgaaca taccacccag ggcgactagg actgtttttt gtgtcggtag cggtccgtca
    34381 ctcactcgtg aggactgtgc tgctatagaa aaaactggct gttcaatcat cgcggttaac
    34441 aattcctggc agatgttcga tgacatttat gccttatacg ccggtgattt gtcatggtgg
    34501 aagcaatacg gatccaccat accgggaggg aaattccgca aagtgacagc caacctggcg
    34561 gcggcgaaat cattttcgtt ggagtacagg cgatattgtg gaccggcgga aggggtaaat
    34621 agcggcgcgc aggctatcag tctggctgct gaatcagggg ctgaagtagt tgtattagtc
    34681 ggctatgact gttctctgca aaacggcctt cattggcatg gcgcgcaccc tcaagcccta
    34741 cggaatccaa cgcaggtgtc tatttcaaaa tggcaacagc agttcctgga tacccgcaaa
    34801 aaacacgcag atttacatat tttgaatgca agtaggagca gtgcaattca atgtttccca
    34861 agaataaatt tagaggcagt gatcgcgtta ttatcgtcgg cagtggccca agcgccgcaa
    34921 actttgttgc gccgcgcgga gtgccgatta tagcggtcaa tggggccatc gactggctga
    34981 accgcgcttc ttattttttc acacttgatc catcgccaga caatatgcgg cgcgttggtc
    35041 gtggccgccg tcgccgtggt gtttgttatt gcatggcact acccgatgtt aaagaacgtg
    35101 aagtcagaga cggcgttctg tgcttccgtc gtgtggctga acgtggcatg gagccaaaaa
    35161 atatgaattc tcccgagtgg tgggcgtggc gctggtccgc acatttcgga ctttgcgaag
    35221 atgagaatga aattgccagc ggcaatagtg catatggcgc tctgaacctg gctttccata
    35281 tcggattcaa acatgtcgcc ctggtgggcg ttgacgctac acaagaacca cgcgttcact
    35341 ccggcggcac gccaaaaaat ctaagtcacc tgcctttgtt attccagtct gcgcgtgaac
    35401 agattgacgt tgtttcatgc gggaaaatgg gaggtattcc gcagatgact cttaaagaat
    35461 ggctgaagaa tacatgatgg cacccacaat ttatcaccgt atcgacggta ccaaatacag
    35521 gaatgtctgg gttgttggtg atctgcatgg ttgctacacc agactgatgt ccgaactcca
    35581 tcgtgtggat tttgacccgg cgcaggattt actgatatcg gtcggcgacc ttatcgatcg
    35641 cggtactgaa aatgtcgaat gtctggaact attgcagatg ccctggttca gggcagtgat
    35701 ggggaaccat gagcggctga tgattgatgc gttaagtcca gatggcaacg tgaataactg
    35761 gctaatgaat ggcggacaat ggttcttcat gctggacact gatcaggaaa tattagcctg
    35821 ggcgctggtg gagctggtaa agcgtctgcc ctatatcatt gagttgaaca ccgggcaaga
    35881 aactatcgtt atagcccatg ccgactatcc ggataatgaa taccaattcg gtaaggaggt
    35941 gccgcttttc aacgttgtct gggcgcgcga gcgtatcagt gattcgatgg atgatattgg
    36001 tggcgaaatt tcgggcgcag atcgttttat ctttggtcac actccggtga aaagcccgaa
    36061 gacattctgg aatcagcagt atatcgacac tggtgccgta ttttgcggaa acctgacatt
    36121 gatgaaagtg aaaggtgatg gtgcagcatg aagattgctt tagtttttcg ctctggtggt
    36181 gactataacg cttccgatgt gcagtggctg gttaatcaac tgccaaaagg ctatgaaatt
    36241 atttgcctga cagacctgaa gcgtttacat gtacctggcg tcaaagttgt cccattgatc
    36301 aaccagtggc aaaagtgccg tggctggtgg gcgaaaatcg agttgttccg accggatata
    36361 acagatgatc tgttctatct ggatttggac acagttattg ccggtgatat acgcccaatc
    36421 cttgagaatc caccaaccag cttcaccatg cttagggatt tttaccatcc acaatatcgt
    36481 ggtagcggtg ccctgtggat accaaatagt gtaaaagcgc atatctggag ttcattctgg
    36541 caagatccgg aaggttggat ttctcgttgt gtcactactg agtgctgggg tgatcagggg
    36601 ttcttacgga aggttatggg cgatgataca ccagcatttc aggatctgta tccaggatgg
    36661 tttgtaagtt acaaggccga tgttgtggaa cctggttcaa aatatgcgag cgcgcgttac
    36721 tccaggggga atggggcatt accaaaggac tgccgaataa tctttttcca cggcaaaccg
    36781 cgacctcgcg aagtgtcaga ggattggctt ccccttatta gctcgttttt tgagcgagaa
    36841 tcagaataat attgctctaa taattccata tttttaaaac gtgatgtaca ctcatcacgt
    36901 tttttattag agcaatccaa aaggtgcact atgtggccat tccgacggaa atatcactac
    36961 tggctgatcg cctttgttac gccgaccggc ggtatcaggc atgtcatcac caggtatcgc
    37021 aacaagagac tcaccttagc cagaatttta caggctgcca taggtgaggg actggataca
    37081 aattgcgtag tccttccacc ttcatactta ggaaaaatga ccgaagcaca agctaatacg
    37141 gaactttgaa atgagcactt cagcacaaaa ccaatcaatc gaaaatgtat ctatccctga
    37201 tgtcctgaat gccggtatcc cggccattat ccagaacatc cgggccgcgc aacgccgcgt
    37261 tagttgtgat gacctcacag cacgtttttt tgataatgcg gttcagtcag cggagatgct
    37321 tcacgcacag cttattgatg tttataacgc agaagctgat agccataact ccctggtaga
    37381 tgcagctgaa aatatgcagt tggatctcgg tctgaagggt aaagaaattg aagagcttca
    37441 gctgcaaatt gaacatttga aacgccagca acaggacgcg atcgacgatg cgacgcatga
    37501 cgccaaccag cgtgctgata atgccgaacg tataagcatt gagctggaaa caaaactcaa
    37561 tgaaatgacc gcgatggttg aactgcggaa ctcacagatt tcaacgctaa aatctcaata
    37621 taaagagatc atgaaacttg atccttttaa ccttgagaaa cgctataaca aagctaaaag
    37681 cgagcgacag gaactgcgta agcaggtcgc cgaccttaac caacagctca aaaaaactat
    37741 taaagatgca agcgaagcgc gcgtggcatt tgctaataaa aaagcagagg ttaccgcgct
    37801 ggttaatgag aatgccaaat ttgcgacgct caagaaggaa atgtatggca ttactgagcg
    37861 ccgtttccct gcaagcaaac ttcatccgac gttagggcaa atctccttct tcccgcgcct
    37921 cctggcttat gggatctcat cgcctaaaga gttcaataac gagcgtcctt atatcgtttc
    37981 taagctggac tttgcttatc agttctgctg cgacatgggc tatgccattg atatccggat
    38041 caacgaatgg ttgatgccaa acttccagcc gttggccatt ttccgcgagt tccagccgga
    38101 aggttgggta gagttcttcc atgaattgat ctgtaaagag atggaaagcc gccgcccgga
    38161 actggtccgt cgagttgagt gggcgcaaga ggttatgttg gcagaggcag agctgccgtt
    38221 cgaaccggaa ttcattgatg aactggccaa taaagggctg catagcctat ttgatgtagt
    38281 tacccgccgt catgagcagt tggttgttga attgggttta gaggaaactg cggcaagaag
    38341 acttctcgat gtttgctatg cacgtagcga tgcatgggaa aaagagaacg gcggcactat
    38401 ttacgttcgc tgatagttac agtgtcacat ttaatgctgg tggagtgcgc ccaccagcat
    38461 ttttttcgtc caatgaggag ggcatttgag tattttcaat aaacacgcac accaggaacg
    38521 tccgtacatc gtcatagtag atattgatgg gacgatatca gaggcaacgg aagacaggct
    38581 gcatttactt ccaccacctg gcaaaggtgc attaacagag cactggaacg agtttaacct
    38641 tgcctgtgac actgatgctc ccatcactcc agttattgat atggtgcgcc agttgtccgg
    38701 catttacacc ctctggtttg taaccgggcg ctgtgagata gccagggata aaacacgagc
    38761 ctggttgcgt aagcacgtaa caaatggggc tgagcctttg ctatctatgc gtcctgccac
    38821 cgatgacaga aatgacggtc cagcaaagat tgatctcctg aagaaaattg gtctaagtaa
    38881 aattgcgttc gcgctggaag ataagattga agtggcgcgt gttttcagga gtcacggcgt
    38941 acttacgtta atggtcaggg agtatgaaaa cgcgcttctc caccaacaat aattgctcta
    39001 ataaatcttg atttttaaaa cagagaaagt gaaaataaaa acatgccgca aggcgcggca
    39061 tgtatccaat caatcacagg agctgaaaat atgaacacgg cattcaaaat cattatggcc
    39121 gcgatctatt tctggctgtt ctctatcact tttggcggca tcgtcgcaca cgggtaaggg
    39181 ggatgcatga aaggcgaagt gaaaaagcgc ggcatgattt ttaacgatga gatggtccgg
    39241 gccatcctcg aaggaaggaa aacgcagacg cggcggataa tgaaaaatca gcctgcggaa
    39301 gttggtccag aagcaccagt gatggttaga aaaattggtg caggttttca gtggtacggg
    39361 gctgatggtg taagcagtgt tttcaattgc ccctttggta tcgtcggcga tcgaatttgg
    39421 gttcgtgaaa catgggcgat attaggcaat gaagatggtt gcagtgtgga ctggaacgac
    39481 aacctttgtc gtggcgatga gaagaacgca gcaaggattt atcgggccag ttgtgagcaa
    39541 aagcctggtg attacggttt atggtcgata cccgatgatg ccgactggaa acctcacact
    39601 gtgaatgaaa agtttgatgg tgggtggcgt ccatcaattc acatgccgcg atgggcatca
    39661 cgcatcctgt tggaaattac caacgtgcgc gttgagcggt tgaacgatat cagcgaatgc
    39721 gatgcaaggg atgagggtgt tccgcctgct ggaagtttgc ttcctgatca cccgggaaca
    39781 ttccttactc ccaaggggga tttcgcaatg gccaaggttg cgttccagcg cctgtgggaa
    39841 tccatctacg gcgaagaaag ctggagcgct aatccctggg tttgggtaat cgagtttgag
    39901 cgtattcagt agggcgagcg tatgcaatca gttatttgtg aggaagtcgg cctgaataaa
    39961 gcatccccag cagagttaag agcaagtctc gaaatggcgc atagccttgc tcaaattggt
    40021 gtcaggtttg taccaattcc agttgaaaca gatgaagaat ttcacaggtt agcggcatcg
    40081 gcagcacaaa agctggaaat catggcagcg aaagtagaga aagctgaagg agcgacaaaa
    40141 tgagcaagcc aaccgacgaa gaaatagttc gggtgttgga agaacacggg cgctgtatga
    40201 cttacgtcgt gaccaactgg cttagggata aatatcgcac actcaaaacg gcatacgttt
    40261 tgcgtcgtct aaagaaactg gagttcgacg ggaaagtaaa gcgcgttaac agttcttata
    40321 taagacaaat ttgctgggag gcatccagtg agtaaccgtt tttacatgat gtgcttgcgt
    40381 gaaactgtgg gtaataacgc ctcattccat tgccataacg gcaatggtta cagttctgat
    40441 atcgatcgcg ctcatgttta cacgctggaa gaagcccaaa aagcctggaa ttgtgggcga
    40501 gatatcgatc agcctgtttg tgccgatagt gtggatgcaa tggctgtgtg gcacgttgat
    40561 tgccagtaca tccctacaga aagcctgatt gagtcagatt gcactgcgta tgtggcctac
    40621 aaaaaaggta gctggaacgg caacgatgtt tactggcttc aacacggtgg attgccaaca
    40681 gatgacttca gtaaagcgac catctttagc gtcgccaaca aaaacgaacc aggaatagtt
    40741 tggttgccat tttccattgc tgatgcagca aagcgtcgga cgttcaatat caataacttt
    40801 aaccgcagaa caatggttca gggcgcaggt ttggtcatgc ctgactggtt gaaagagcag
    40861 aacagaagaa agaagtcgcg aagcgggaag gtgcgttgga attgtccgca ttgcggaaaa
    40921 ataacctggc agtacagccc atatgatttt gaaggctgta gtgattacaa ctgtgaagga
    40981 tggcgagaat gacaattgac tatcaggcac tgcgagatgc ggcagttgca gtagaaacag
    41041 aacctatgca tcaaaatttt gtggcgtttc gtatggcgtt cacgccatcg gtggcactgg
    41101 cactactgga tgaaattaag cggctggaag acacaaatat tgatgctatg tgccgtattg
    41161 cagagcttga gactaatctc gcggcgctgg tggcagagaa cgccggactg aaacacgcaa
    41221 tggccgtaac tcttgagcat gtgtcggtca cggatgcagg gcaggctggt gttgctgcaa
    41281 tgattatcaa cgatgcccta caccacagcg aaactccagc taccgatgct tttctggctg
    41341 aaattcgtgc ggaagcacgc aacgagggga ttaactatac cgccagccgt cttgctgctg
    41401 cgttcaatca cggatttatc aataagtctt tgcgtgaagt tttcgacgtt acacgcatga
    41461 ttttgtcagc gaaagaagag ttggctaatg aagcgcatcc gattgatggc ctgtctggtg
    41521 aatatgcgga gaaatccctt gaagaatggg cggaacagat tcgcaaagga agcagccagt
    41581 gaatatcgac acgacaataa cgatcgatac gctcctaaat accggtctgg cacttctcgg
    41641 ttggcttttc atcatgtccc gtacatggcg atggctgggt tccattttcc taaaacagtg
    41701 gaaaaaacgg cgcaaacagg aactacgcca gaaggcatta gaagcgttct atgacgcatt
    41761 tgagcttagc cgcattgaac caggtacaac agctaggata gcgacaaaag gcgacctgat
    41821 gatagtgatg ttccgacagg agagagcaga gaaaggggaa tcagcatgaa attttccaaa
    41881 ttttctgagt tggtgaatcg tattttgttc aacaaccaca gccatcgtcg cgatatggat
    41941 gtaacaatcg ttgttcattc gcctggcagc attggttcaa caccctcagt tgaggttcag
    42001 tcaattcacg ctggttttga ttgggattcc gggacagtgc tgattttccc agcacagcca
    42061 ctgaccacgc taacaccaga acagattact gatattactg atagtgtgcg caaaggtcag
    42121 tcctggcacg catatcagga atacaagaag cataaagagc agttggaaaa attatcgatt
    42181 gaacttgatg ccgcaaaaca gcgcattgca gagctggagg gtaattgcgc ggcgctggcg
    42241 gcggagaatg cggcaatgca tgaaactatt gaagccgttc ggggtgttgc ggataactcc
    42301 agtggaattg ccggatggca tttgaatggc gatatcgcta catgggaaga gattcttcct
    42361 gaaattaacg atatcgaaac cccagccacc gatgcttttc tggctgaagt acgtgcgcag
    42421 ggcgtggaga tgctggcaaa aaatcatcag agtatcgtca atgcacttaa gggagattct
    42481 ttattttctg atggtgaata ccgacatgca gctattgttt ccgcagctgt atatttcgct
    42541 gcagagcttc gcaaaggagg caaccagtga gcgaaattaa ttaccaggca ctgcgtgagg
    42601 cggcagaacg tgcaactcca gcaatggaac gcctgttaat gttgccagtt gatgatgatt
    42661 tgttaagtga acaggaactt aaagattacg gtgtggatat tgatgcgctc aacgccttca
    42721 aatttctgac cggaccagaa accgtgctgg cactactgga tgaacgggaa agaaaccagc
    42781 aatacatcaa atcacgcgac caggagaacg aggagattgc gcttacggaa gggaagctgc
    42841 taatcgaaaa cggccggctt gttgccgata cgctacgcca cttagctgat aacgaaatcg
    42901 actctgatta ttttgctatc acctcaacga atgagaacgg tactgaaatt gatcatgaga
    42961 tggctattac cgattacgca ctgcaagctg ccggaactgt agacgaattg gttgcggcgt
    43021 tggaatccgc agagaatcgc attgcagaac tggaagcacg ggaaatatcg ctcccagaac
    43081 gtagcagcat gcttcatcga acagattttc acgatgatta ccaaacggta atggcataca
    43141 aagtttctga agtcatcgct gcaatccgcg ccgctggcat tcgcatcaaa ggagaggagc
    43201 atggaaataa aaccagaaga tgagttaagc aatatcgttt tatttccggt aaaagaggat
    43261 gacccacgta atcaggttaa ttttctctat gagccatcgg aaagaccata ttgccatcac
    43321 gcctctgtcc gggttgacga aaaagagcgt caggtccgct gtaaaatctg cggtgcagtt
    43381 gtggagccat ttgactggat gctctctgtg gcgaaaagag aaaccagact ggcagatgat
    43441 gtaaggatct tgcgccagga ggaacgggaa aggcggagaa atatagaaaa gctaattcag
    43501 attgagcgta acgtgaaagc gcggatacgc agggtgacaa aatccagaac tgaataatta
    43561 aatttagctc tgttaaaaat ttaatcctta accggaggta tatcaatgtc aaacgcacag
    43621 aaagttatta acgctgaaaa atataacgag tgggtgaaaa agttctcaga gcagattttt
    43681 aaaattactg gcgacgagaa tgcggcaaaa aatgaattag agccgtggac acctgaaggt
    43741 gtcgacccaa attattgctg gtgggatgtt gatccagttg atgctgcaaa tgaagctatg
    43801 agttatcaca acgattaatg tcaggaggcc gcccgaaagg gcggtaatga aaagtgactg
    43861 aattaaccaa agagaaatta atcgaagaag ccaaattaaa aatagcgatt gcgaaatgct
    43921 accccaattc agggatggca cgggtatagg gcgagttatt caaaattgca ctggcatagc
    43981 tggaagcaga tccagttaaa cgagttaact cagatcagat gcaccgagtc tgcttagaag
    44041 ctaatcgcta tttagataaa tatgacgcga tggcgaaaga ggtaaataag ttgcttggac
    44101 gcatcgcccc gccagcgcca gtatttaacg gcgaatacgg tgacgcatat cagggcgctc
    44161 gtgaagacct gtccatctgg aaacggcgag cgcttgaagc tgatgagcac gttcggcgac
    44221 tggagcaaat caatgaccac atggtgaaag aggcgcaggg agaatcacgc atgggcgagc
    44281 ctgtaatacg tgagccagca ccggtagtgc ctgaagaagc aactccggaa aacgtagaaa
    44341 tgctctctgg ctatgtttca acgtacaaat taaccgatag cgagcgcgat attgctgccg
    44401 aaatatggaa cgcctgccgc gccgccatgc ttcaggctgg aaactttcgg gaaaataagg
    44461 gttcgtcaac caataatttt cgggaaatct cggaaacgtc aaccaactat ccggtaactc
    44521 cggatggttg gataagctgt agtgagcgaa tgcccgctca agatgattgg attttaattt
    44581 attcaaagca cggtgagtat atggcaggac aggtgcaagg ggaatacgtg gagttgagcg
    44641 acggcacttt atcgtggtta gggaacgcct tgttctggat gctgctacca gaacctcccc
    44701 aggaggcagg tcaatgagct ggcctgaagc attcgcaacg gtaggaattg tgatggcggc
    44761 agcactgggt ttgtattcaa tttgtcgctg gtggtaacga tggggaaatt aacttttgta
    44821 gtcgaatttg aggatggtaa agagccacct gttagcgcca atcttgatgt tgctggtggc
    44881 aggcttgttt cggttctatt tggtgactac cgagatgatt tcttccaacc agaagaagtt
    44941 gatgtggtgc gagaggcatt aaacgagtta agtgttgata acgatgatgc tcatgcggaa
    45001 atcatccaaa aaatggaact gctaactcac taaattatca attatggtgc tatcacctac
    45061 gacaccgaga gaaaatttat aatgtcaaaa gtaaatgttt tgattttttc agcaattgtt
    45121 ggctttggtt ttactgccgg agtgcagatt tatattgcgt gggaaaaaat catcaactac
    45181 gcatggagtt gttttattaa gtgaggtaag tatgtggaga ggtaatagtc atggcaaaag
    45241 ccagatgata cttaccgaat ataagctaga ccacaaaacc aataaatcac gttcagtata
    45301 tttgcttcgc cacaatagcc gcgtaaggaa taccgtactg gagcaaaatc tgaccgttga
    45361 aatggataat tacgggggct tcaagccaac aatatcgctt gatgattttc ctcgtggttt
    45421 aagcgaaaga gaagcaatgc tgaaattagc agaatggcta caaagattaa gcattgctat
    45481 tgaagataac tggtctgaac cttaaattta tatgatgaca ctaaaacatt ttcttgaccg
    45541 cccattatgg gcggcagccg caggctatga ctttaattat atggattgca tgtcttatac
    45601 tgccaatgca tacgaccatt cgttcagcct gctgtttaat tctttaagaa tattgccgca
    45661 aacagaagtt ggagagcttc atttatggct attgggcttt atcgcggctg gagttggtat
    45721 tgctgtatgg ccttttattt tctggctggt ggctgttgta gtgtggttta agtgcaagac
    45781 gtaccggaaa aagtatttct taggtgatgg aatgactgat attgccaaaa tgaacattga
    45841 aaaatggact aaggaatgtg aaaagaaatg gcgcaaaaag aaatgactac tctaacgaca
    45901 gcatacttac agcaattggt attttttgca ggcgaggcta cttgtcatcc tgacgcaaac
    45961 tatttattgg aatttgagag gttagcgtca cccggtatcg ttctggaact ggcccaacag
    46021 gtgctggcct taagacaaaa agagcaacat gaaagtaata cgtgtagatt gaattttgag
    46081 cagtggctgg aacagcaacg cggaaaaatc gatgtggact gtggttgtgt gtctactgaa
    46141 acattcatgc actggctgcg ggtagcttac gaggctggca actatccgga tattccggat
    46201 agttcggtgc cagcgccagg aaagggcgtc accggtgaac gtatccgcat taagccgcat
    46261 gtttatcgcg aactggttaa ccgtctccac gatacagcga tcaagtgtgc aggcacccgg
    46321 caattacgag aaaaaattag ccgtgttttg ggcgacgtta ttacgccaga tcatcataaa
    46381 caagccgaga aaagtggcct ggaaaggtgt caccttgagg cggcattaaa cattaagccg
    46441 gggcatacgc ttggcattat tgatgcacta ttggttcata agatggccag ggctttattg
    46501 ccgctggtgg ctgataagca tgaggcgggc catgccaacg aaagctgagt tacaggcgcg
    46561 catagagatt cttgaaaaag agaatgcgag tctaaaagga atgctggcgc gggcggaaag
    46621 ggaattatca ggcaaattat tgccagaaga actgccacca gcagatatac cagatcgagt
    46681 gtcccggtgg atgaagtctt ttggtttacc gtgggaggct ttttggtgct acgagcatcg
    46741 cagatggtgt gatgaacttg atagcagttt cccctacttt gcggaaggga acacctgccc
    46801 tgagtgtagg aggtagcgcg atgtttcgtc ctcgtccaat accgccgaaa ataactaatg
    46861 gctatcagcc gaatactcca gaaatacaag ggagcgaggg gcttaaacca ccaccagcac
    46921 cacgtattcc gcctgtagca aggaacgtat cattacgacg agagttaata ttagccttgt
    46981 cgcccgttct ggctgcccgc caggattggg taagtaatga tgacctggca gaaaaaatca
    47041 catcgctggt ggataaaatt ctgcagattg gcaatgagat tacgctgatc cattggtgat
    47101 gaatagtagc aaagcgcaca aaatcatctg cggtggttgg tgtacataac gagtttgcac
    47161 caaaggtgtc tctttaatgt atactgtata aatgaacagt attgttgagg tgaaaacgct
    47221 atgggcttcc cttctcctgc ggcggattat gttgaaagca gaatttctct tgatcagcaa
    47281 ctaatcaggc atccatcagc gacctacttc atgcgggcgg cagacagcca tcaccgtgag
    47341 ggaatattgc agggtgcttt gctggtggtt gattcctcgc ttactccggt tgatggttcg
    47401 ctgcttgtct gcgctatgga gggtgaatat cgcataaaga gataccgaaa gtatccgcgc
    47461 cagcacctgg aggatttaag caccgggaag aaagaggcgt taccagtaga tgacgatgga
    47521 tacacgggca gtaatgctgt ttttggtgtg atcactcatg tcatcaatga tgcccgaagt
    47581 ggggagtttg atgattgtcc ggttatttaa gctgcaaagg gctggtgctt tatgcctgtt
    47641 aggtttataa ttgtgtacac ataacgagta cacgaggtgt ttatgcaatc cattaacttc
    47701 cgtaccgcgc gcggcaacct ttctgaagtg ctcaacaatg ttgaagccgg ggaagaggtt
    47761 gaaatcaccc gcagaggccg tgagccagca gtaattgtca gcaaggctac tttcgaagcc
    47821 tacaaaaaag cggcgctgga tgctgaattt gcatccctgt ttgacaccct ggactccacc
    47881 aacaaggaac tggttaaccg ataatgaggc atatatcacc ggaagaactt attgcgcttc
    47941 atgatgcgaa tataagccgc tacggcggcc tgcctggcat gtcagatccg ggcagggcag
    48001 aggccattat cgggagagtt caggccagag ttgcctacga agagatcacc gaccttttcg
    48061 aagtctccgc cacctacctg gtggctacag cgagagggca tatattcaat gatgccaata
    48121 agcgtaccgc gctaaacagc gcgctgctat ttctacgccg taacggggtg caggtatttg
    48181 attcacctga actggcagac cttactgtag gcgctgcgac tggcgagata tctgtatctt
    48241 ctgtcgccga cacgttacgt agattgtatg gttccgcgga gtagattaat ggcacgtaaa
    48301 tacaacaaat tgtcccgtga agcgttaaag atgcttcttg atggcgtgag tcgccgcgag
    48361 gtaaagcaat acctggttgg taagcaaatt ggtgccagga ccgctattgc tgtgttatgc
    48421 cgtcaggaaa tggttgtgct taaacagaga atgccgggca gcagataaag cccaatcagt
    48481 gatgaaaggt gtgatgtgaa agccgtaatt actccctttg tacaaaaaga gcttggcgtc
    48541 gccacattca aagtggatca ggaagtcaga aagctggtgg aggctggccg taaatttatt
    48601 atggagccgg tgccgcgtga gttaatcgag cacatggacg acggcctcgt tgtttccgag
    48661 caaactatgg caacaaatga ggcgttgcag ctgtttttta acagcgatga actgtttcgc
    48721 cgtattggtg gaattgacgc gctggtggcg tggttgcgca ggaaagaggg gcaatgccag
    48781 gccgcagatc gtagctggtg tgacaaccat attgtccacg cagaacgaga caatagcgcg
    48841 gtgttgttgt gctggcatca cgataaccat taccggatgc gtggttttaa tgagctgaaa
    48901 gaaacgctgc ataataatcg cgttaactgg atactggatg tcgcccgtca ggaaatgggg
    48961 ctttcagatg gccatgattt aagtattcag gaactgtgct ggtgggcttt catgcgcaac
    49021 atgatgcacc tgatgccgga agaagtttgc cgtatatcaa taaataagat gaaagccgca
    49081 acgcaggata gcggacctct gaaagaggcg gatattcgcc cgtatgacga tcgcgctaca
    49141 gcatatgttc agatgatgga agaacgcgcc gcgccgatgc gtgcaaaagt atgccctgtg
    49201 gatgttgact ccgaccctgg catggcgcat ttcaaaatac caaaactgca atcgctaaaa
    49261 ttacctgagt acatggactt tgttgcttcc cgtccatgct gtgggtgtgg agcggcggga
    49321 gctggcgctc acattacgcc ttatatcgtt cgtcatagtc gattatgcgc gcatgacatt
    49381 tacgcaattc ctctgtgcca gtcatgccag cgtgatattg agcgtgaccg cgataattgg
    49441 gagaagacgc acggtaggct ggcgatgcat caacgattgt tctttgatta cgcgcttgga
    49501 gtcggcgcta tcacaagtca ctcgtcgagc gttagataaa attgctctaa tgtattgcga
    49561 tttctttaat cgagggtatt atattccacg ttgattagtt gacatgggct aatcagtagg
    49621 tgacaggatg ttacttaact ggcagggacg ccacttcatg gaaataaatc actcacgaat
    49681 aacatcgtac gagattgcgg attacatgat ccgcactaaa tctcttctat cagcgaaaga
    49741 actcgcagca attcttgaaa aggaataccc gcatctggat gtcgataagc gcgatgttta
    49801 tctgcgctta aaggctatcg ctgtgtctaa gtattcgtct gttttgattg atgacagtac
    49861 acgcccacgt agatttcaga tccactctct gaatcctgaa ttctttcgcc gtagccgcgc
    49921 tccgcgccgg tttgatgaaa aactccagaa cgaactctat atgacgcagg acgaaaagga
    49981 acgccgggag caccagcctt gggtaatggc gcgtcaactt ttcaataagg tggcccgtca
    50041 gcaccgtcat tacggtaatg ccacatccgc acgtatctga ttgattgctt gcccgttccg
    50101 ggccttttga catgtgactt tcgttaccct cgcgtcaaaa agagttttta cgaaaggaag
    50161 cataagtgac ctgggacgat cacaagaaga attttgctcg cctggcgcga gatggtggtt
    50221 acaccatcgc acagtatgcc gccgagttta atcttaaccc taatactgca cgtcgttatc
    50281 tccgtgcctt caaagaagac accaggacag cggacagccg caagccaaat aagccagtca
    50341 ggaagccact aaaaagcatg atcattgatc actctaatga tcaacatgca ggtaatcaca
    50401 ttgcggctga aatagcggaa aaacaaaggg ttaatgccgt tgtcagtgcc gcagtcgaga
    50461 atgcgaagcg ccaaaataag cgcataaatg atcgttctga tgatcatgac gtgatcaccc
    50521 gcgcccaccg gaccttacgt gatcgcctgg aacgcgacac cctggatgat gatggtgaac
    50581 gctttgaatt cgaagctggc gattacctga tagataacgt tgaagcgcgg aaggccgcgc
    50641 gcgctatgtt gcgtcggtcc ggggccgatg ttctggaaac cactcttctg gaaaagtctc
    50701 tttctcatct ccttatgctg gagaacgcca gggatacgtg tattcgtctg gtgcaggaaa
    50761 tgcgcgatca gcaaaaagac gatgatgaag ggactccgcc tgaatatcgt atcgcgagca
    50821 tgctaaacag ctgttccgcg cagataagca gcctgatcaa caccatttac agcatccgga
    50881 ataactatcg aaaagaaagc cgggaggcgg aaaagcacgc tttatctatg gggcaagctg
    50941 gcattgttaa gctggcatac gaacgaaagc gtgaaaataa ctggtcagtg ctggaagcag
    51001 ctgaattcat cgaggcgcat ggcgggaaag tgccgcccct gatgctggag caaatcaaag
    51061 ccgatctgcg tgctcctaag accaataccg atgatgagga aaggcaaaca gccgtcggtg
    51121 gcccttctct tgaagatctg gacaaagttg cgcgagaacg ggccgccaac cgccgcgcgg
    51181 atgccgcatt gtggattgag cagcgtaggg aagaaatcgc cgatatcgtt gatacaggcg
    51241 gttatggaga tgttgatact gaaggtgcat caaacgaccc atggctggaa caagacctgg
    51301 acgaagacga ggaggaagac gaagaagtta cccgcaagct atacggggat gatgattaat
    51361 ggccagaagt tgcgtaacgg atccacgttg gcgcgagctg gtggcgctat atcgttatga
    51421 ctggattgcg gccgctgatg ttttgttcgg caaaacacct acctggcagc aggatctgat
    51481 tattgagtct gtgcaggaac agggtagcaa gacatctgtt tcgtctggtc acggtaccgg
    51541 gaaatcagac atgacttcta tcatgatcat gttgttcata atcatgtatc ccggtgcccg
    51601 agccattatc gttgcgaaca aaattcagca ggtaatgacc ggtatattca agtacatcaa
    51661 gataaactgg gctactgcca ccagccgttt cccatggctt gctgattatt ttgttctgac
    51721 agaaaccgct ttctatgagg ttactggtaa aggtgtatgg actgtagtac cgaagggctt
    51781 tcgtctggga agtgaagaag ctctcgccgg tgaacacgca gatcatcttc tgtatattat
    51841 cgatgaagcc tccggtgtca gtgatagagc tttcggtatc atcaccggtg ctcttaccgg
    51901 acaggataac cgcatcttat tactgtcaca gcctacacgc ccaagcggct atttctacga
    51961 tactcaccat aaactggcca agcgtcctgg taaccctgat ggcgtttata cggcgatcac
    52021 gcttaacagt gaggaatcac cgctggtaac gccagcattt atcaaaatga agctggcgga
    52081 gtacggcggg cgcgataacc ctatgtacat gattaaggta cgcggcctat tccctaaatc
    52141 acaggatggc ttccttcttg gacgtgatga ggttgaacgt gcaacgcggc ggaaagtcaa
    52201 gattgccaaa ggatggggct ggcttgcatg tgtggacgtt gctggtggta cgggaaggga
    52261 taagtccgtt atcaatatca tgatggtgtc cggccagaga aataaacgcc gtgtaatcaa
    52321 ctatcgaatg ctggaataca cagacgttac agaaacgcag cttgccgcca aaattttcgc
    52381 agaatgtaat cctgagcgat tccccaatat caccatagcg atagacggcg atggcctggg
    52441 taaagcaacg gcggatctga tgtacgagta ttatggtatt accgtacagc gcatacgctg
    52501 gggtaaaaag atgcatagcc gtgaagataa gagcctgtac tttgataagc gtgcttatgc
    52561 caacgttcaa gccgcagagg ccgtaaaatc aggtcgtatg aggctggata agggtaatgc
    52621 aaccattgag gaagcgtcta aaatacctgt aggtattaac tccgcaggtc aatggcgggt
    52681 gatgactaag gaagacatga agaaaaagct caacctgcac tcaccagacc attgggatac
    52741 atattgtttc gctatgctgg cggattatgt tccccaggat gaggtgctta gcgtcgaaga
    52801 cgaagcgcag gttgatgaag ctctggcatg gcttaatgaa tgaatatttg ctctaataaa
    52861 ttgtgttttt taactaccga tgttacattg aacctgacct cttgcgcctt gaggcatttt
    52921 cggtttatgc ttatcaggca cctcattaaa acgggtgccg ggattgagac cccggataat
    52981 gtcaaaggcg acacagacgc cgaaagcgtc tttttttgtg tcatgccatc gcacagccat
    53041 acgtagcgtt tagctccgaa atcaatggta gtgctggctg ggctgccgaa aggcaggccg
    53101 gttccctttg acgccggtag tctcaaccca gtcagtgcta ccgccattga gattgagacc
    53161 tcacgcggta gctccttaac ttagtcaaag gaggctgcca ttatggctac tgtccctacc
    53221 ccaactcatc ctgaattcat ctggcgcttc tattcctgcc agaaacgtca ctatcacttc
    53281 gttattgcac cgacagaaga tgaggcacgt tctcagcttc cggatgctcc ctgtattttc
    53341 tctgcccgct tttccactga ttcgcgcaac tctctcagtt actggtgcct ccctgttaac
    53401 gcttctgctc aggagggact atgagaacgt cattagtcac ccgtgaagaa atgattgagg
    53461 caattgaaca gcacacatcc tgtatcagta ccagggatat accgggcgtt attgccaact
    53521 acttcatgat caccaaacaa ctttaccgga gaaaggacaa gaacgcggtt caccgtatcc
    53581 tgttgtctga tatccgcgaa tacctgctcg aacagggtca tctgaattac gcaaccgtcg
    53641 cagccgaagc acgcaaggag gcacacagaa tgaaagcaaa taacgttaaa ttagaaaaaa
    53701 ttcatgcacc ttcagttcag gaatcggagc tggtggttgt tcagaatcag tctgatgaaa
    53761 ttcccgttct ggaatggcag ggagtgcgtg tagtgacaac cgagactctt gcgagagggt
    53821 atgggacaga aacaatccgt attcgccaaa atcatcatga gaacaaagta cgctttgttg
    53881 aagggaagca ctttttcaaa gttgaaggag aatcattgcg cgagttgaag cacagagtag
    53941 ctttaaacta ctctgtaaaa attgctcgca atgttcgctc actcaccctc tggacagaac
    54001 gcggcgcagc ccgccacgct aaaatgctcg aaaccgatca ggcatgggca ttctttgaaa
    54061 aactggaaga cagctacttc cggcaaaaag aacagcaacc ggttgcaatc ccccagacgc
    54121 ttccagaagc tctgcgcctg gctgccgaac tggctgaaca aaagcaactt ctggaacaga
    54181 aagcccacca gctaaatcag cagctggtgg ccgccgctcc taaggtcgat tttgccgacc
    54241 gggtatcagt ggccaagggg atcctgattg gaaattttgc aaaggttgtt gggcttaagc
    54301 aaaacgcgtt gtttgcctgg ttacgggaga acggcatcct gatagcgtcc ggtggacgta
    54361 aaaatgtgcc gttccagcaa tacatcaacg ccgggtattt cacggtgaaa gaagtggtgc
    54421 tggatgatga agatggttac cagatacggc tgacgcccca attaacgggt aaaggccagc
    54481 agtggttaac tcgcaagcta cttgatgctg gtttgttaaa accagtagcg gctgagtgat
    54541 taaattaaaa aggcggcctt tcggccgcca gtgatttcag ataccccggc agccgccggg
    54601 gttatttttg gtggtcaatt aaacggattg attgaattat taaacgtgat gatgcttgtc
    54661 tcacgcggtg cctggacgtt agccgcttgc ggaacctcct taattttctt ggtgacaggc
    54721 aagttgcgtg cgccaacttt gatcagagat tcgaaaagtg tcgcaacgat ttttgcatca
    54781 ccaggttctt tgaggcggaa tgcgtctttt tgggcggcgg agacgaagat cgggaggtta
    54841 tccagttcgt cttgcattgc tgccagcaca tcgtcgcgga tacccgctgt ttcctccagc
    54901 aaagcaattc gcgcttcagc atctgcgatc ttggccattg cttcaaggtg gcgaccctgg
    54961 ctttcgagta gtgcggtttc cagttcagcc gtacgctctg tcgcctccac catcatttcc
    55021 agttcagcca ttttactgta atgggatata acggcctgca ctgactcgtc ggagtaccca
    55081 tgcgccgcca gggactctgc cagtaaagat ttagaatccg cgctttcaaa cattccggcg
    55141 ctggcaggat gatccagact gatatagttc ggcgttgtca cataatccac accatggaag
    55201 ctggtggtta cagcgatttt cccggactca cgcccgccag tggcccagct ccagccacca
    55261 gcacggcttt cgatcatagc ggcgacaatt ttacccggct ctgtgttaag aatttcctgt
    55321 gtatgggtaa cgatgccgtt gtcgtcaaca gatatagcca ctgttcggca cgctggaaca
    55381 ttgtcgatta cgaccgggcg accttccacc atgatcacgc tggtttctgg tacttccagt
    55441 ttgccggtca gctgtcggcg accgtgaccg taatagccga aaagctcacc aaggcgtaaa
    55501 ccttcctgag tttccttgct ttcaagcatg gcctttaccg cgcttaatac atactgtcgc
    55561 ccgttctggc gaccttttcg agcattgcta tagagacaaa agcggtcagt gaccgttttc
    55621 aaaacatcag ccattattgt ttccctcttt aaagaccgat tcaaggattt gcgccagttc
    55681 ctgtggcggt gttttgatga tggaatccat caggtgatcg tcgtcctcgc ttttagcttt
    55741 cagttcgttc accagggctt cagagatttt ttcgtcaatc tccagcacat cgctgaacag
    55801 gtaacgtttg aatgcatcgg aattggcgag gacgctgtta ttgctgacgg catcgaggat
    55861 ttgcgtaacg atggtggcgt agttcgcctg cgagtcgcgg ttatcgttgt gctcttgttg
    55921 cagagcggta ttaacggagt ggaattcgat tttgtacggg cgatcacctt ccgggtatac
    55981 cttgccgtac ttgaaagcaa gatgaatatc gatagcccgc tgaatgaact cttctacgcc
    56041 ctgctggatc catgaggcgc gcatggcggc ctgaatcgcc gtgcgcagga atccaccttc
    56101 accaagcccg ccggacattt gatctgccca ccccaggagg gtgtaatcga ggccaagtgc
    56161 tgccgccagc tggcgcatat aagtgagaat gtcttcaatg ccgttgatgt ctgcctggat
    56221 ggtctgagta tcaatggtca tctgcccctt gccgtcgccc ataataggca gcagggtatt
    56281 ggtgaccgta ggcatgttat tcgcgccgcg tgcgcgcttt tccatcaggt cagctgctcg
    56341 tttaagcgtc tgagtaatgg tgcgtgaata atcggctgct tttaccggat ccagactatt
    56401 catcgccaga ccgatgattc ggtcaatttt cgacgcatta aaacgcgttg ccttcagcga
    56461 gcggatcgcc gaacgcaggt tcatgtacgg ctcgtaggcg tattcgagca agctggtccc
    56521 gtaattctgg gtttcaatcg gcgtgcgctc ttccggatta tccagcaggc tgtaagcctt
    56581 atggccagtg tgcacaggca taaggtttga cttaggccgc cagtagggga ttttcatagg
    56641 gataatggtc cacggatcgg cgaaaaccat tttccctgac gcgtccttca gataatcgcc
    56701 gctaaatccc gccaggttac cgctgacctc gaactccttg atgaagctcg gaagggtgta
    56761 ataggagcac tcaaaagacg tgatccctat gccttctttg gcgtatggcc tgacataagc
    56821 caccccaaat acagacatga taaatgccca cccggcgacc tctttgttga tggttcgccc
    56881 gatgtcgttc atcagctcgt cacacaaccc ctgcgcggcg tcatagtcac tatcgtttcc
    56941 gttgtgtacc ggcacgatag agaaggtttg tccggtcttc ttatcgaaag agagcgcgtg
    57001 cgtaatatgg atgttcagcg cggtggcgat cgtgctgtaa accgccatct cttcgagtag
    57061 cggatagcgt tgcaagcggt cttccggcag ttgaacttca tcaaagataa agcgacttcc
    57121 gtccaccagc ccatcgccag ccatgccact atcgcccggt ttgccgccta agaagccgga
    57181 cagttgtacc ggtgcccctg cgcgagaaaa caaataccca cttccgccgt gcacagccag
    57241 cgcggacagg aggatgttgt cccgttctcc gttgtcttta aaaacccccg ccagcgcctt
    57301 cctgaccgag gatagcgtga ttttattgtc tgccaagatt gcaccttaat tagaataatt
    57361 cgcatcgtgt ttgaacggaa tttaacacta gtcacttgtt aaggattacc aatgaacaag
    57421 ctatctatgg gggtgtttcg ctgttcaagt gtcagcgaaa tattgaaata tattagtgca
    57481 ataacatttc atcgggcgcc gattagatac ggcgtggaaa aggtggaagg caaaagctat
    57541 gaccgactac gccggacgtg aaataatatg tccacactat ctgatacaat aaaaccgaat
    57601 aggacataat ttcgacgtca aagccttggc acaggctgga gagaaaactt gaaaaagcga
    57661 tactacacag taaagcatgg gacgctacga gcattacaag agtttgctga caagcataac
    57721 gttgaggtgc gaagggaagg gggcagtaaa gctctgcgca tgtaccgtcc ggacgggaaa
    57781 tggcggacag tcgttgattt caaaacaaac agtgttcccc agggggtccg cgatcgggca
    57841 ttcgaagaat gggagcagat catcatagat aacgcattgc tcctgaatgc agattaaact
    57901 ttccgccctt ttgcccggtc tatcgccggg catctttcag agataattac ctttacccgt
    57961 caattcccct tgtggtactt gtttttgcgc cagtgtttgg gttccgtgaa ttatgttaat
    58021 cagaggtctt agtaacgatg gttcctggcg ggcctcaact tctccagcca ttgccctgat
    58081 gtagtcggcg ctggcaacat tgttgtattc cgttgcgaaa cagcataaca atgtcagaac
    58141 atgctctgtt gttatttcgc tccagttgat gttgaaaaat tcatcgcctt ttttatcgtg
    58201 ttcggaatcg aagatgcttt ggtggaggat gtatttgccg gattccttgc gcggtaactt
    58261 gatcgctttc tggcgttcca gctccttgta aatctgcatt gcttcaatta gtaccggcct
    58321 gccgttcatg aagggatcgc gcaaccttac acgctggcca actcgaccgg taataaagct
    58381 gttttcctct tccaccagca cgataaaacc cttttcctct ttttctcgca attcgcgcag
    58441 cagctggagt tccatatcgc ggcggcgttc agggtagctg gtccgctcag ccatgatcag
    58501 ctcattgttg atccatgcag cagtcattga cgccggtttg ccgacgctca ttgaaacaac
    58561 gcatattttc ttatccatag cgcccctaca aaaaagaaaa gccaccagcg gcggcttagc
    58621 aatacaactg aaggtagcgc ccggtactca gactgtgccg tccatggaat atttgaaaag
    58681 ggatccatcc gtaccgggcg tgtgatgatt ctgactgaag tcacttgtca gttgtcaatc
    58741 atttatcatt aaaaataata tatttattag tgcaagatgt ttgccatctc ataggcatca
    58801 gccagtaact ccatctctga cttgttcagc aaggtgaatt ctttcttgcc tccaaccaca
    58861 ccatcggcat gaacagggac cagccatggg tatttttctc ttacttcagc cggagctgca
    58921 tgctggtggt gccatctaca aagtggcaat tgctttttat gacaacccgg cgcggtacga
    58981 ccggcgatat ggtgcagaga cacctcatta gatattactc catgcatata gcaggcaatg
    59041 caggggagag cgccaagagc attggcgatg cgccgttcct ccgccgttgg tgttctcccc
    59101 ttcaagccac gagattttat ttttaccgcg cttttccgcg ttttgctggc tggtgggcgc
    59161 tctttctgtt tagcgatacg gcggtcgata gaatcccgca ttttctgata ttgcgattct
    59221 ctccaggcgg ggtcagccaa cttttcccgt tgccgagcga tcgctcgttc tctggctgcc
    59281 ttctgccact cgcggcgctg ttcaagtttt tgttcgattg ttttcatatg gcaaaaaaaa
    59341 ggcggcctaa tggccgccaa tgatgtcaag gagttaagta atggcaacgt cttcgtagtt
    59401 gacaaaaact gcggctcaat tatagcaatc aattagagca atggtagata ttttgttaat
    59461 cgagaatcac attttttcac ttcagtacct gtgtgctata ctctttcttg attgattgga
    59521 tgcggaatac aaacccgctc ttttgtgcag cctggctcct tgccaggctt ttttatttca
    59581 tcatggaagc tgttaacgct ttggatcttg ctgaactgat tgaaagggca ttgtttacct
    59641 tacccaggag ttcgccaaat tccgccatca ctctagtaag ccctcgccgc gcttcctcct
    59701 ccgttgcatt catcacaaaa tgttcagcac tccgcatgct tttaacgggg aacgcaacag
    59761 atatcgagtc gatatcaggc atcctatcgc tcaattttac ggtgacaatg acagatggtg
    59821 actgaatttg agagcttaca gacagcacca catattttcc gtctattttg aaatccttcc
    59881 gcatgtgtca ccataaatat caaataatta gagcaatcaa gtaaaaatga acggctaatc
    59941 gccatcttcc agcaggcgca ccattgcccc cgtttcacta tccaggttac gaatgtagtt
    60001 catgacaata tttacgttgg tccagccacc agcttgcatg atctccggta ttgaaactcc
    60061 ggcgcgggcc atatctcgcg cggcaccgac acgggcacta tgtccagacc aggccaggta
    60121 tcgctgacca gagtcatctt ttgccccgta aatcaatcgg tgagttgctt caaaaatccc
    60181 ttccagggcg cgagttgata gctggctggt ggatgatggc gcggcaacac cattttttct
    60241 gacgcggcaa aacaggtagt tattcggatc atcagctaca ccagagacag aaatccatcg
    60301 ctcaaccagt ttagttaccc ccaggctaag tgccttctct acaccagcgg tgctaaccag
    60361 cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg agatatcttt
    60421 aaccctgatc ctagaaattt cggctatacg taacagggtg ttataagcaa tccccagaaa
    60481 tgccagatta cgtatatcct ggcagcgatc gctattttcc atgagtgaac gaacctggtc
    60541 gaaatcagtg cgttcgaacg ccagcgcctg ttttgcacgc tcaccggcat caacgttttc
    60601 ttttcggatc cgtcgcatga ccagtgaaac agcattgctg tcacttggtc gtggcagccc
    60661 ggaccgacga tgaagcatgt ttagctggcc caaatgttgc tggatagttt ttactgccag
    60721 accacgcgcc tgaagatata gaaggtaatc gcgaacatct tcaggttctg cgggaaacca
    60781 tttccggtta ttcaacttgc accatgccgc ccacgaccgg caaacggaca gaagcatttt
    60841 ccaggtatga tcagaaaacg cctggcgatc cctgaacatg tccatcaggt tcttgcgaac
    60901 ctcatcactc gttgcatcga ccggcaatgc aggcaaattt tggtgaacgg ttaacgaatt
    60961 agtcatttat tatgcgtgat tttaacagtt aagccttgtt catgaagtat ctcatcaata
    61021 attcgaatca catcccctgg tagatcatcg ttctcgaaat atctgtgatc ggggatttct
    61081 acagtgattg gggttgctgc tttatgcaaa atccgaacaa tctttttttc ttttttgcat
    61141 gttgcatatt cagcctcttc gatatctatc cagctcgcac catcaaattg ctggtaaacc
    61201 cgagtgtata gtaagcggtt tcgctttact tcttctgcta actcccaaat tgcaacagcc
    61261 atagcacgtt cattttcatt cgttccaggg ttagcagctt tttgttttgc gtcgttcagt
    61321 aaggcgttta ccttggcatc tgtcaggcca ttaaacattc atcacctcat gatcaagtta
    61381 aattattgtt ttcagcagag tgtacaggat tggctctgcc tttacctggt tatggttctc
    61441 gtcatagaaa cgccagcgac cgcgcgtgcg ttctattttc tcttcaccgc gagataatga
    61501 cagttggtaa ctatcacgct caaaccattt tgcccgccag taaccacggt ttttctcaag
    61561 ctcaagatga gtggacactt tagcagctga atatcccatt tttcacctct gattgattgg
    61621 tggtgctaag tgcgctacgc gaaatctgga gcactaacac tgccaacatt tcgcagattt
    61681 tacgtagcgc aaccttgatc aaatgatcaa gtgatcacta tttgacctga taaggtattg
    61741 aactgtatgg atttacaggt aaattaatca tgttcaataa cccttaagat aacttcgtat
    61801 aatgtatgct atacgaagtt atcagatccg aagaggagtt tacgtccagc tgcgcacaaa
    61861 aatcaagaat tattagagca ataaatttag agagaaaaat cccactccac cagctaaaaa
    61921 ctggattgtt tttcatagtt gtttgacaat tgctctaata aattatagtt ttgccgtcgt
    61981 tacgtaatac gactttggat tcactattta atgtgtcttc agcgttgtag agcggctcag
    62041 aaggaaatga gcaaacaggg aaaccttata caacggcatt acagctatgc attgctcatc
    62101 ttacacacag cgcaatgttg ttagattacc ccagcatgga tcatgggtga aacagtaggt
    62161 cagagcttca ggctctgtgt tgtcaataca gtgaggcata attatggctt tcattcaacc
    62221 aaccatcgac gacgttagac attgctctaa cgctttatct gtagaccctg ccgaaaccga
    62281 cgctgcccgc gccattgctg aacactactc aaagatatcc aatcaggagt accgcatcac
    62341 ccaagacgac ctggatgacc tcactgacac aatcgaatat ctcatggcaa ctaaccagtt
    62401 agactcacaa taaatgcact aataaatcta ttatttttgt ttgatccctc tataatatag
    62461 gtcagtaatg accggttttc tcagccgggc gttattgacc atgtcaattc tggaggagga
    62521 tcaatgataa attatgtcta cggcgaacaa ctgtaccagg agttcgtcag cttcagggat
    62581 ctctttctaa aaaaagctgt tgcacgcgcc caacacgttg atacagccag cgacggtcgt
    62641 cctgtacgcc cggttgtcgt tctaccgttc aaagaaactg acagcattca ggctgaaatt
    62701 gataaatgga ctttaatggc gcgggaactg gaacagtacc cagacctcaa tatcccaaag
    62761 actattttat atccagtgcc taacatcctt cgcggtgtgc gtaaggttac aacttatcag
    62821 acagaagctg tgaacagcgt caacatgacc gctggccgca ttattcatct gattgataag
    62881 gacattcgca tccaaaaaag cgcggggatc aatgagcaca gtgcgaaata catagagaac
    62941 ctggaagcaa caaaagagct aatgaagcag tacccggagg atgaaaaatt ccgtatgcgc
    63001 gtacacggct ttagcgaaac aatgctgcgc gtccactaca tttccagtag ccctaactac
    63061 aatgatggta aatcagttag ttaccatgtg ccactgtgtg gcgtgtttat ctgcgatgaa
    63121 actctccgtg atggaattat catcaacggt gaattcgaaa aagcaaaatt tagcctttat
    63181 gactccatag aaccgatcat ctgcgaccgc tggccgcaag caaaaatata tcgcctggca
    63241 gatattgaaa atgtaaaaaa acaaattgcc atcactcgcg aagagaaaaa ggttaagtca
    63301 gccgcatcag ttacgcgcag ccgtaaaacc aagaaggggc agccagtaaa cgacaccccc
    63361 gaaagcgcgc aataaattat gcccggcatc aaccgggcat tcttccatta ttcagctgcc
    63421 accggtttta acaagccagc atcgagcagt ttacgcgtca accactgctg gcctttaccc
    63481 gttaattgag gcgtcaaccg tatctggtag ccatcttcat catccagcac cacttctttc
    63541 accgtgaaat accccgcgtt gatgtactgc tggaacggca catttttacg tccaccggac
    63601 gctatcagga tgccgttctc ccgtaaccag gcaaacagcg cgttttgctt aagtccaaca
    63661 acctttgcaa aattcccaat caggatccct ttagctactg atacccggtc ggcaaaatcg
    63721 actttaggag cggcggccac cagctgctga tttagctggt gggctttctg ttccagaagc
    63781 tgcttttgtt cagccagttc ggcagccagg cgcagagctt ctggaagcgt ctgggggatt
    63841 gcaaccggtt gctgttcttt ttgccggaag tagctgtctt ccagtttttc aaagaatgcc
    63901 catgcttcat ttgtgtccac gatcttagac atgcgtgcag cgccgcgctc tgtcca
//
LOCUS       DAZFKM010000010        63026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFKM010000010 DAZFKM010000000
VERSION     DAZFKM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1739
                     /gene="adeD"
                     /locus_tag="ACHKAY_000740"
     CDS             <1..1739
                     /gene="adeD"
                     /locus_tag="ACHKAY_000740"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO5699458.1"
                     /translation="HISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVI
                     KGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGL
                     TTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQ
                     MLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYIT
                     AGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDR
                     NPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIV
                     LLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQF
                     TPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGF
                     GLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPI
                     AGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDG
                     EKFAFTTLEVTE"
     gene            complement(1784..3175)
                     /gene="uhpT"
                     /locus_tag="ACHKAY_000741"
     CDS             complement(1784..3175)
                     /gene="uhpT"
                     /locus_tag="ACHKAY_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO5699459.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3431..4750)
                     /gene="uhpC"
                     /locus_tag="ACHKAY_000742"
     CDS             complement(3431..4750)
                     /gene="uhpC"
                     /locus_tag="ACHKAY_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO5699460.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            complement(4760..6262)
                     /gene="uhpB"
                     /locus_tag="ACHKAY_000743"
     CDS             complement(4760..6262)
                     /gene="uhpB"
                     /locus_tag="ACHKAY_000743"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO5699461.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6262..6852)
                     /gene="uhpA"
                     /locus_tag="ACHKAY_000744"
     CDS             complement(6262..6852)
                     /gene="uhpA"
                     /locus_tag="ACHKAY_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO5699462.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6927..7217)
                     /gene="ilvN"
                     /locus_tag="ACHKAY_000745"
     CDS             complement(6927..7217)
                     /gene="ilvN"
                     /locus_tag="ACHKAY_000745"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5699463.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7221..8909)
                     /gene="ilvB"
                     /locus_tag="ACHKAY_000746"
     CDS             complement(7221..8909)
                     /gene="ilvB"
                     /locus_tag="ACHKAY_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO5699464.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9015..9113)
                     /gene="ivbL"
                     /locus_tag="ACHKAY_000747"
     CDS             complement(9015..9113)
                     /gene="ivbL"
                     /locus_tag="ACHKAY_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO5699465.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9678..9767
                     /gene="tisB"
                     /locus_tag="ACHKAY_000748"
     CDS             9678..9767
                     /gene="tisB"
                     /locus_tag="ACHKAY_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO5699466.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10030..10104)
                     /gene="ysdE"
                     /locus_tag="ACHKAY_000749"
     CDS             complement(10030..10104)
                     /gene="ysdE"
                     /locus_tag="ACHKAY_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO5699467.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10186..11370
                     /gene="emrD"
                     /locus_tag="ACHKAY_000750"
     CDS             10186..11370
                     /gene="emrD"
                     /locus_tag="ACHKAY_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO5699468.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11378..11875)
                     /gene="yidF"
                     /locus_tag="ACHKAY_000751"
     CDS             complement(11378..11875)
                     /gene="yidF"
                     /locus_tag="ACHKAY_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO5699469.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11872..12234)
                     /gene="yidG"
                     /locus_tag="ACHKAY_000752"
     CDS             complement(11872..12234)
                     /gene="yidG"
                     /locus_tag="ACHKAY_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO5699470.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12224..12571)
                     /gene="yidH"
                     /locus_tag="ACHKAY_000753"
     CDS             complement(12224..12571)
                     /gene="yidH"
                     /locus_tag="ACHKAY_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO5699471.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12681..13130
                     /gene="yidI"
                     /locus_tag="ACHKAY_000754"
     CDS             12681..13130
                     /gene="yidI"
                     /locus_tag="ACHKAY_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO5699472.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13177..14670)
                     /gene="yidJ"
                     /locus_tag="ACHKAY_000755"
     CDS             complement(13177..14670)
                     /gene="yidJ"
                     /locus_tag="ACHKAY_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5699473.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14667..16382)
                     /gene="yidK"
                     /locus_tag="ACHKAY_000756"
     CDS             complement(14667..16382)
                     /gene="yidK"
                     /locus_tag="ACHKAY_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO5699474.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16549..17442
                     /gene="yidL"
                     /locus_tag="ACHKAY_000757"
     CDS             16549..17442
                     /gene="yidL"
                     /locus_tag="ACHKAY_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5699475.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17439..18253)
                     /locus_tag="ACHKAY_000758"
                     /pseudo
     CDS             complement(17439..18253)
                     /locus_tag="ACHKAY_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18253..19869)
                     /locus_tag="ACHKAY_000759"
     CDS             complement(18253..19869)
                     /locus_tag="ACHKAY_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5699476.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20165..20881
                     /gene="yidP"
                     /locus_tag="ACHKAY_000760"
     CDS             20165..20881
                     /gene="yidP"
                     /locus_tag="ACHKAY_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5699477.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20878..22539)
                     /gene="yidE"
                     /locus_tag="ACHKAY_000761"
     CDS             complement(20878..22539)
                     /gene="yidE"
                     /locus_tag="ACHKAY_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO5699478.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22736..23164)
                     /gene="ibpB"
                     /locus_tag="ACHKAY_000762"
     CDS             complement(22736..23164)
                     /gene="ibpB"
                     /locus_tag="ACHKAY_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO5699479.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23276..23689)
                     /gene="ibpA"
                     /locus_tag="ACHKAY_000763"
     CDS             complement(23276..23689)
                     /gene="ibpA"
                     /locus_tag="ACHKAY_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO5699480.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23683..23755)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23995..24327
                     /gene="yidQ"
                     /locus_tag="ACHKAY_000764"
     CDS             23995..24327
                     /gene="yidQ"
                     /locus_tag="ACHKAY_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5699481.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24329..25543)
                     /gene="yidR"
                     /locus_tag="ACHKAY_000765"
     CDS             complement(24329..25543)
                     /gene="yidR"
                     /locus_tag="ACHKAY_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO5699482.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25644..26708
                     /gene="cbrA"
                     /locus_tag="ACHKAY_000766"
     CDS             25644..26708
                     /gene="cbrA"
                     /locus_tag="ACHKAY_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO5699483.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            complement(26705..28042)
                     /gene="dgoT"
                     /locus_tag="ACHKAY_000767"
     CDS             complement(26705..28042)
                     /gene="dgoT"
                     /locus_tag="ACHKAY_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5699484.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28117..29265)
                     /gene="dgoD"
                     /locus_tag="ACHKAY_000768"
     CDS             complement(28117..29265)
                     /gene="dgoD"
                     /locus_tag="ACHKAY_000768"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO5699485.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29262..29879)
                     /gene="dgoA"
                     /locus_tag="ACHKAY_000769"
     CDS             complement(29262..29879)
                     /gene="dgoA"
                     /locus_tag="ACHKAY_000769"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO5699486.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29863..30741)
                     /gene="dgoK"
                     /locus_tag="ACHKAY_000770"
     CDS             complement(29863..30741)
                     /gene="dgoK"
                     /locus_tag="ACHKAY_000770"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO5699487.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30738..31427)
                     /gene="dgoR"
                     /locus_tag="ACHKAY_000771"
     CDS             complement(30738..31427)
                     /gene="dgoR"
                     /locus_tag="ACHKAY_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO5699488.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31705..32361
                     /gene="yidX"
                     /locus_tag="ACHKAY_000772"
     CDS             31705..32361
                     /gene="yidX"
                     /locus_tag="ACHKAY_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO5699489.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32407..33219)
                     /gene="yidA"
                     /locus_tag="ACHKAY_000773"
     CDS             complement(32407..33219)
                     /gene="yidA"
                     /locus_tag="ACHKAY_000773"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO5699490.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33334..33732)
                     /gene="yidB"
                     /locus_tag="ACHKAY_000774"
     CDS             complement(33334..33732)
                     /gene="yidB"
                     /locus_tag="ACHKAY_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO5699491.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33972..36386)
                     /gene="gyrB"
                     /locus_tag="ACHKAY_000775"
     CDS             complement(33972..36386)
                     /gene="gyrB"
                     /locus_tag="ACHKAY_000775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO5699492.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36415..37488)
                     /gene="recF"
                     /locus_tag="ACHKAY_000776"
     CDS             complement(36415..37488)
                     /gene="recF"
                     /locus_tag="ACHKAY_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO5699493.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37488..38588)
                     /gene="dnaN"
                     /locus_tag="ACHKAY_000777"
     CDS             complement(37488..38588)
                     /gene="dnaN"
                     /locus_tag="ACHKAY_000777"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO5699494.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38593..39996)
                     /gene="dnaA"
                     /locus_tag="ACHKAY_000778"
     CDS             complement(38593..39996)
                     /gene="dnaA"
                     /locus_tag="ACHKAY_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO5699495.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40293..40373
                     /locus_tag="ACHKAY_000779"
     CDS             40293..40373
                     /locus_tag="ACHKAY_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699496.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40603..40743
                     /gene="rpmH"
                     /locus_tag="ACHKAY_000780"
     CDS             40603..40743
                     /gene="rpmH"
                     /locus_tag="ACHKAY_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO5699497.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40760..41119
                     /gene="rnpA"
                     /locus_tag="ACHKAY_000781"
     CDS             40760..41119
                     /gene="rnpA"
                     /locus_tag="ACHKAY_000781"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO5699498.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41083..41340
                     /gene="yidD"
                     /locus_tag="ACHKAY_000782"
     CDS             41083..41340
                     /gene="yidD"
                     /locus_tag="ACHKAY_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO5699499.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41343..42989
                     /gene="yidC"
                     /locus_tag="ACHKAY_000783"
     CDS             41343..42989
                     /gene="yidC"
                     /locus_tag="ACHKAY_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO5699500.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43095..44459
                     /gene="mnmE"
                     /locus_tag="ACHKAY_000784"
     CDS             43095..44459
                     /gene="mnmE"
                     /locus_tag="ACHKAY_000784"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO5699501.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44702..44776
                     /gene="tnaC"
                     /locus_tag="ACHKAY_000785"
     CDS             44702..44776
                     /gene="tnaC"
                     /locus_tag="ACHKAY_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO5699502.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44997..46412
                     /gene="tnaA"
                     /locus_tag="ACHKAY_000786"
     CDS             44997..46412
                     /gene="tnaA"
                     /locus_tag="ACHKAY_000786"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO5699503.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46503..47750
                     /gene="tnaB"
                     /locus_tag="ACHKAY_000787"
     CDS             46503..47750
                     /gene="tnaB"
                     /locus_tag="ACHKAY_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO5699504.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47882..49057
                     /gene="mdtL"
                     /locus_tag="ACHKAY_000788"
     CDS             47882..49057
                     /gene="mdtL"
                     /locus_tag="ACHKAY_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO5699505.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            49032..49991
                     /gene="yidZ"
                     /locus_tag="ACHKAY_000789"
     CDS             49032..49991
                     /gene="yidZ"
                     /locus_tag="ACHKAY_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO5699506.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            50148..50897
                     /gene="yieE"
                     /locus_tag="ACHKAY_000790"
     CDS             50148..50897
                     /gene="yieE"
                     /locus_tag="ACHKAY_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO5699507.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            50919..51485
                     /gene="yieF"
                     /locus_tag="ACHKAY_000791"
                     /gene_synonym="chrR"
     CDS             50919..51485
                     /gene="yieF"
                     /locus_tag="ACHKAY_000791"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO5699508.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51539..52876)
                     /gene="adeP"
                     /locus_tag="ACHKAY_000792"
     CDS             complement(51539..52876)
                     /gene="adeP"
                     /locus_tag="ACHKAY_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO5699509.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            53043..53708
                     /gene="yieH"
                     /locus_tag="ACHKAY_000793"
     CDS             53043..53708
                     /gene="yieH"
                     /locus_tag="ACHKAY_000793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO5699510.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53775..54248
                     /locus_tag="ACHKAY_000794"
     CDS             53775..54248
                     /locus_tag="ACHKAY_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699511.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            54297..54884
                     /gene="cbrC"
                     /locus_tag="ACHKAY_000795"
     CDS             54297..54884
                     /gene="cbrC"
                     /locus_tag="ACHKAY_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO5699512.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(54946..55668)
                     /gene="yieK"
                     /locus_tag="ACHKAY_000796"
     CDS             complement(54946..55668)
                     /gene="yieK"
                     /locus_tag="ACHKAY_000796"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5699513.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(55683..56852)
                     /gene="yieL"
                     /locus_tag="ACHKAY_000797"
     CDS             complement(55683..56852)
                     /gene="yieL"
                     /locus_tag="ACHKAY_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5699514.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56879..58495)
                     /gene="bglH"
                     /locus_tag="ACHKAY_000798"
     CDS             complement(56879..58495)
                     /gene="bglH"
                     /locus_tag="ACHKAY_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO5699515.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(58581..59975)
                     /gene="bglB"
                     /locus_tag="ACHKAY_000799"
     CDS             complement(58581..59975)
                     /gene="bglB"
                     /locus_tag="ACHKAY_000799"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO5699516.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59994..61871)
                     /gene="bglF"
                     /locus_tag="ACHKAY_000800"
     CDS             complement(59994..61871)
                     /gene="bglF"
                     /locus_tag="ACHKAY_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO5699517.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(62005..62841)
                     /gene="bglG"
                     /locus_tag="ACHKAY_000801"
     CDS             complement(62005..62841)
                     /gene="bglG"
                     /locus_tag="ACHKAY_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO5699518.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT        15416 a        16701 c        15796 g        15113 t
ORIGIN      
        1 atcacattag ccgggctgaa taccaggaat tgttagccgt ttcccgtggc gacgctgttg
       61 ccgattatat tattgataat gtctctattc tcgacctgat caatggcgga gaaatttccg
      121 gcccaattgt gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatgctc
      181 cggctttgca gcggattgat gctcgcggcg caacggcggt gccagggttt attgatgctc
      241 acctgcatat tgaatccagc atgatgacgc cggtcacttt tgaaaccgct accctgccgc
      301 gcggcctgac gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggagaagccg
      361 gattcgcctg gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca
      421 gctcttgcgt acccgccctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac
      481 agatgctcgc ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc
      541 ctggcgtaat tagcgggcag aatgcgctgc tcgataaact ggatgcattt cgccacctga
      601 cgctggacgg tcactgcccg ggtttgggtg gtaaagaact taacgcctat attactgcgg
      661 gtattgaaaa ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg
      721 gcatgtcgtt gatgatccgc gaagggtccg ctgcccgcaa tctcaacgcg ctggcaccgt
      781 tgatcaacga atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg
      841 agatcgccca tgaaggacac atcgatgcct taattcgccg cctgatcgaa caacacaatg
      901 tgccgctgca tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga
      961 atcacctcgg cttactggca cccggcaagc aggccgatat cgtcctgttg agcgatgcgc
     1021 gtaaggtcac ggtgcagcag gtactggtga aaggcgagcc gattgatgcg caaaccttac
     1081 aggcggaaga gtcggcgaga ctggcacaat ccgctccgcc atatggcaac accattgccc
     1141 gccagccagt ttccgccagc gactttgccc tgcaatttac gcccggaaaa cgctatcggg
     1201 tcattgacgt catccataac gaattgatta cgcactccca ctccagcgtc tacagcgaaa
     1261 atggttttga tcgcgatgat gtgagcttta ttgccgtact tgagcgttac gggcaacggc
     1321 tggctccggc ttgtggtttg cttggcggct ttggactgaa tgaaggtgcg ctggctgcga
     1381 cggtcagcca tgacagccat aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc
     1441 tggcggtcaa tcaggtgatt caggatggcg gcgggctgtg cgtggtacgt aacggccagg
     1501 tccaaagtca tctgccgttg cccattgccg gcctgatgag caccgacacg gcgcagtcgc
     1561 tggcggaaca aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg
     1621 agccgtttat tcagatggcg tttctttctc tgccagtgat ccccgcgcta aaactaacca
     1681 gtcaggggct atttgatggc gagaagtttg ccttcactac gctggaagtc acggaataat
     1741 aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc actgtcaact
     1801 gctgaatttt tttctcgcgg cggattttgc gttcttccat caccgccact atcgccatca
     1861 ggcagataca accaatcgcg gcgatatcaa gtgcggcgaa ggtgcctgcc cagccggtaa
     1921 ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca aagctgtcac
     1981 caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct tttttaggta
     2041 caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg aaacccaacg
     2101 caaagagaga agccagatag atatattcgt tgctggcatg ttgatacaca ccgagcgtgg
     2161 cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc gccaggtcag
     2221 agagccagcc ccacagcagc gtaccgacca gtgcaccagc ttcaaacagc gtaaagccct
     2281 gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt gaccactggt
     2341 cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc cagatcactt
     2401 tgtttttcag cacatactca acaaagatct gccacttggt catatcggta gattctgtct
     2461 ctttgtcctc ttcgctgatc tcctcgccga acagttcttc agctttgccg aggccataag
     2521 attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc gcgataatcg
     2581 acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc ccgaacagcg
     2641 ccacacctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa ccgaggaatg
     2701 tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac gaaccgccgg
     2761 tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg ctaaccgagc
     2821 cgctgcccat actggcactg aagcccagca tacaaatggc agacaggatc agcatgaacg
     2881 gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga aaccagcgtt ttacccacgc
     2941 cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc aacccgtagg
     3001 tcgagatcat gtcgttctgc gcgatgttaa agttcttgcg aatcaggtac atcgtcaggt
     3061 agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt ttgcgccgca
     3121 cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taagaaagcc agcatgggtt
     3181 actcctgatt tcccttcata cttcaagccg caggtgcctt agctgcacgc attcctcccg
     3241 gtcacatagt tcactatgct cccgggactc acgcgtttgc tgcgttcctg caacttgaat
     3301 tattttggga aagaattaat gaatgatggc attgtaaaaa tcagcagcaa atcaggcctg
     3361 agatagcgtc caggcaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag
     3421 gtgagatgca tcacgcttcg cgcggtgcct gggcgttcaa aaagggcagt aacagcagtg
     3481 cggaaacccc ggcggcgata gagataacca caaaaaatcc gctccagtgc caggtatcga
     3541 gtactttcgc cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa
     3601 accccgtcgc cgcccctgca gcctctttgt gggaacactc tgccgccgcc atgccgatta
     3661 acatctgcgg gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt
     3721 agctggcaaa tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa
     3781 tcaaattcat cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca
     3841 gcgcaccgat aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat
     3901 cgacgcccag cgtctcggac atatacaaat tgccccagtc gttgattgcc gcccggacca
     3961 catagaccag cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt
     4021 tggtcaggat ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg
     4081 cgtcgtgccg ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc
     4141 gccagcagag aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac
     4201 gccagccgta atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt
     4261 tatgcgccgt gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg
     4321 ttaacagacg cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga
     4381 gcacggcaaa cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg
     4441 cgataagccc tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga
     4501 caaacttcga cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac
     4561 ggctgagcac gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga
     4621 agtaaaacag cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat
     4681 agcgggcatc aatttcatat ttatcagtca ttaatggcgc atcggcaggc gctttcagaa
     4741 acggcaacat catcaaacct tagacatagc gttgaggtag agaaacgctg acacgcgtgc
     4801 cgtgcagaca ggaaatgtgt aatgtgccac ccagcgccgt tacgcgctcg cgcattccgg
     4861 tgaggccaaa accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaataa
     4921 ccagcatcaa ccgttcatcc tgctgccagc cttgcagggt gaccgcgctg gcatcagcat
     4981 gtttcacaat gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt
     5041 cgcttaacgc tgattcatcg attcgccatt cgagatggct gacaataccg cgcccttcca
     5101 gctccatttc ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccaactggc
     5161 gcggacgtaa ccgacccaac aaacggcgca ccgcgtcgta aacgcccagc gatagttgtt
     5221 cgatgagctg cccgctctgc ttcacgctgg cgttatctgc cgccagccgc tgaacaatgc
     5281 ccgcctgagt acgaatggct gtgatggtct gaccgatatc atcatgcagc tcacgcgcca
     5341 catcacggcg cacgctctct tcggtttcca gcaaccgttc agccagatgc tgattgcgcg
     5401 ccagttcctt ttgcagcgac tggttaagtt cacgcaaccg ctggatgcca gcgccaagca
     5461 acaaccctgt cagactttgc accagcagcg agagcaataa atccaccgga tgatcgcgcc
     5521 aggtttgact ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gccccttgcc
     5581 aaccatagtg ccaggccagc gcgataatcg gcagcgccag acaaaatggc gtaaagcgcg
     5641 acagttcgtc cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc
     5701 agaccagatg tcgcccgcgc cagttgatcg gctgagaaac cagtgacggg ccgagcggca
     5761 gccaggtgtt attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcaggc
     5821 cgccagttaa agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca
     5881 gcgactgcaa caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt
     5941 cacgctgatg gcgatagcgc gagatcagcg ccacgggcag taacgtcagt aaactaccga
     6001 tcatcaataa cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc
     6061 actccgcgcc cagcagcacc ggccagtatc cgcgcgggca ttgcagcatt agccccagac
     6121 gcagaccaaa cggaaataac agcaccgcca tatcagggcg ctcaaccagg tgcaggctga
     6181 tactccacag gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta
     6241 agcgggagaa caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc
     6301 gttactgaca cccagttttt ccatcaggtt ggcgcgatgg acgtgcaccg ttttcggcga
     6361 caagcccagt tcggcagcaa tctctttcac cgccattcct tgcgccagtt tttccgccac
     6421 ctgccgttcg cgtttggtta gcgggtcctg acgaccggat gccagtttaa tggcaatatc
     6481 cggcgtcaga taacagccgc ccgtggcaac cgtatgcacc gccgcaatca gttcgtcagg
     6541 gctacagcgt ttggagagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc
     6601 cggactgtca tgaacagaga gcattattgt cgccatacct ttcggcagct ggcttagcag
     6661 ttccagaccg gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc
     6721 cggcagcccc gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg
     6781 ttccagcccc agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc
     6841 aacggtgatc atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag
     6901 cgcgatgttg ttcaagcctt gagcggttac tgaaaaaaca ccgcaatctt gttaaacata
     6961 gtcggatcgg actgattacg ctgcactttc accacatctt ccagcttatc gatttggctt
     7021 atcatctgct ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg
     7081 tcctgaatcg gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca
     7141 caaacgtggg tcattacgcc cggatggttg cgaacggtga gctccagaat cacgttgtca
     7201 tgagttgtgt tttgcatggc ttattccccc accatttcag tattcgccgc acctggcggc
     7261 accatcgggt aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg
     7321 cgattgatga tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt
     7381 tcgaggccga atccggcggc aatctgcata aagttgattt ttcccggata ggtggcggca
     7441 aaaacgcctt gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg
     7501 ttcatcagaa tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata
     7561 ttcatcatca ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc
     7621 gccgcgccaa tcgccgcagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc
     7681 cactggcgtg ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca
     7741 tcggtggtga taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa
     7801 tggcttaatg gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct
     7861 accaactggt gccactctgc acgcggttgc gcttccacct gcgggatcaa ttgcgccagc
     7921 acgtcatcaa catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg
     7981 cgatcgatat cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc
     8041 gcccggtcat caaaacgcgc accgagcact atcaacaaat ccgcctcctg caaaatatag
     8101 ttggtgctgc gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc
     8161 aacatgccca gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca
     8221 cgcacccgtg cgggcgcatt gatcacaccg ccgcccagat aaagcaccgg gcgtttggca
     8281 gcgttaatca tcgccgctgc gtcacgaatg ctttcttcgc taaaggcggg ggcggcggct
     8341 ttttctgcca tagcgggctg tgtttcaatc tcaaaaactg ccgtttgcac atccttagga
     8401 atgtctatcc acaccgggcc tgggcggcct gattgcgcaa tgcggaaggc atcgctcatg
     8461 acctgcggga gttcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag
     8521 atgccgtagg tgtccacttc ctggaagacg tcggtgccga tcatcgaggc gggaacctga
     8581 ccggtgatgc aaatcagcgg gatggagtcc agccgcgcat cggcaatggc ggtcaccagg
     8641 ttagtcgcac ccggtccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc
     8701 atcccctgag cgataaatcc cgcgccctgt tcatgacggg ccagaatatg gcggatttgc
     8761 gtgctttggc ttaaggcatc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca
     8821 atcttaatgc cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta
     8881 cgcgtcgatg ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc
     8941 cgggaggtgc ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg
     9001 tgttgttcca gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact
     9061 gcggcggata gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac
     9121 cttgttggtt ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg
     9181 aaaatttttc agcaagggaa aattgagggg ctgatcacgt tttgtactga attgcaggta
     9241 acaaaaaacc ccgccggagc gaggtttcat cagccgcctg cggctggtaa ccgcaaagca
     9301 cactgtatta tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca
     9361 cgaattttga cgtatttagg gcatagttga gtatcgatca cagtttgcgt tttgtccaaa
     9421 tattactgtt tatttataca gtaaacttct ataatatcac tgtacgcaat gtgttatgcg
     9481 ggggccgcat cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc
     9541 cttaaccccg cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttaagata
     9601 ccgcgtgcac aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg
     9661 aaccacagga gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg
     9721 ttgcagcact gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt
     9781 cgcaccaaag gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagttg
     9841 ggggcttctt ctaccagcag aatgtttgca cctgcgctgc ctgtgcccct caccctagcc
     9901 ctctccccaa aggggcgagg ggaccgtccc gtgcaatgtt tagctcattt tctgaaaggg
     9961 aacggtggtt tccccctcgc ccctttgggg agagggttac agttcgcacg taatccctcc
    10021 cggcaacatt tattgccgct tttgtttaca tattctgccg ctaaacaatt ccccattcct
    10081 ggcgtatatc tggctaacat tcatcaatgt gatagattcc tctcccgcat ttatgggaat
    10141 gcgtagtgac ttattctaat tatttttata aaagcatccg tgataatgaa aaggcaaaga
    10201 aacgtcaatt tgttattgat gttggtatta ctcgtggccg tcggtcagat ggcgcaaacc
    10261 atttatattc cagctattgc cgatatggcg cgcgatctca acgtccgtga aggggcggtg
    10321 cagagcgtaa tgggcgctta tctgctgact tacggtgtct cacagctgtt ttatggcccg
    10381 atttccgacc gcgtgggccg ccgaccggtg atcctcgtcg gaatgtccat ttttatgctg
    10441 gcaacgctgg tcgcggtcac gacctccagt ttgacggtgt tgattgccgc cagcgcgatg
    10501 caggggatgg gcaccggcgt tggcggcgta atggcgcgta ctttgccgcg agatttatat
    10561 gaacggacac agttgcgcca tgctaacagc ctgttaaaca tggggattct cgtcagtccg
    10621 ttgctcgcac cgctaatcgg cggtctgctg gatacgatgt ggaactggcg cgcctgttat
    10681 ctctttttgt tggttctttg tgctggtgtg accttcagta tggcccgctg gatgccggaa
    10741 acgcgtccgg tcgatgcacc gcgcacgcgc ctgcttacca gttataaaac gcttttcggt
    10801 aacagcggtt ttaactgtta tttgctgatg ctgattggcg gtctggccgg gattgccgcc
    10861 tttgaagcct gctccggcgt gctgatgggc gcggtgttag ggctgagcag tatgacggtc
    10921 agtattttgt ttattctgcc gattccggca gcgttttttg gcgcatggtt tgccggacgt
    10981 cccaataaac gcttctccac gttaatgtgg cagtcggtta tctgctgcct gctggctggc
    11041 ttgctgatgt ggatccccga ctggtttggc gtgatgaatg tctggacgct gctcgttccc
    11101 gccgcgctgt tctttttcgg tgccgggatg ctgtttccgc tggcgaccag cggcgcgatg
    11161 gagccgttcc ccttcctggc gggcacggct ggcgcgctgg tcggcggtct gcaaaacatt
    11221 ggttccggcg tgctggcgtc gctctctgcg atgttgccgc aaaccggtca gggcagcctg
    11281 gggttgttga tgaccttaat gggattgttg atcgtgctgt gctggctgcc gctggcgacg
    11341 cggatgtcgc atcaggggca gcccgtttaa gcgcacgtca ccgcagcatc gtcatcagct
    11401 ccatgggaga acgatgctgc tttatcagat cacgcatcac ccgcatatgc ggtgcggagt
    11461 aagaataaaa cgcctgatag cccgcacaaa gcacgctgtc tggcgtctcc tggcgagcgt
    11521 gcgggcacag gcgatgccag tcgcacgcct ggcagtcacg gcgcggttgc gggttttcgc
    11581 tgccacacca tttttgtaag gtggcgtcaa aaagctcaat cgagatacga ctgatatctg
    11641 cccgcaccca acactcgaac agggcaatta aaaatttccc ccacagctct gctgtcaccg
    11701 aagcggccgt cagatcgccc cggtcatcaa attcaagata gggaatgagc tgtacggagc
    11761 ggatgccccg ctgtttgaaa ttgtgataaa aatcggcagg ttgcagagca tccttgtact
    11821 ctacaaccag tttatgcctg tcctcttcag catcaatgac ctgacttcct gtcatgcgac
    11881 tctctcaata aatacgataa gttgatgtat atgcgttaca gcaaacagta ttgcgagaga
    11941 taacaccgcg agggagatca aaaatttgtc acggactacg tgaaattggg aaaaatcgct
    12001 attcgtgaca tccattaaat tgcgattacg ggtgtagtgc cagaggatca gcgccacgat
    12061 ggcgagaatg ccaatcgaaa tccagaataa catgcccgcc tggtgccagt tgtgtttgat
    12121 agccaacgcc atcaacgcgc cgtagcccag catggtacga aaccacgcca gtgatgtgcg
    12181 ctccggctgc agtcctgggt cggcaatgcg tctggctttg cggctatccg gcatacaaca
    12241 ccagtcccat aacgatcacc gccacgacca tcaatattaa gctaatgatt aataagctgt
    12301 tggtgtaggg caggtcctct ttcagacgca tcgctttttc attgcgtaac cagcgcaggt
    12361 agccatacat cgctaacccg ccggaaaaca gacacaacaa caacgccagc agttcacgaa
    12421 tgacgggcgt ggcgaaatct ggcgcaagtt gatcaagccc aacccccgcc gccagaaatc
    12481 ctagtgcggt acggatccac gccagaaaag tacgctcatt agccagcgag aagcggtaat
    12541 cgggcgcttc tccaaggcgg gaaattttca ttgttgctcc tttgcaattt cactaacgct
    12601 ctacctttag cggttttttt ttaagaattt cgtccgcccg tttcctgata tgctttcgcc
    12661 atccgctaca aggaggtcag atgggtatca tagctcaaaa taaaatcagt tcattgggca
    12721 tgctgttcgg tgccatcgct ttaatgatgg ggattatcca cttttcattc ggcccttttt
    12781 ccgcaccacc gccaacgttt gaaagcatcg tggcggacaa aacggctgaa attaaaagag
    12841 gtcttctcgc cggaataaaa ggcgaaaaaa ttaccaccgt ggagaaaaaa gaggacgtag
    12901 atgtcgataa aatcctcgat caaagcggta tcgcactggc cattgctgcg ctactttgcg
    12961 catttattgg cggtatgcgt aaggaaaatc gctggggcat tcgcggcgcg ctggtttttg
    13021 gcggcgggac gctggctttc cataccttgc tttttggtat cggcatcgtg tgcagcattt
    13081 tgctgatatt cctgattttt tccttcctga ccggcggttc actggtttaa gcagcctgaa
    13141 taatgcccgc cgggtgttca tcagcgggca ttatcgtcag aacttctgtt ttttctcttc
    13201 cactttcact ccttgcgtcg gtaggccggt gtcatagtca cgtaccaccg gcgaatagcc
    13261 atcttgtggg cgtggacgaa atgcccccat ccagcgcggc agtgcatctt tacgccacgg
    13321 acgcaggctc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt aatccaataa
    13381 ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca ggttatgcat
    13441 ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt tgagtaccag
    13501 tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc tgtcatgctc
    13561 aatctcgtag cggttaaatt ccaccattac accgcgtggc tctttcacgg caaggatatt
    13621 ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg tcggcagtaa
    13681 atcgatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg aacggatgat
    13741 cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca gcttatgtgc
    13801 gcccatcatt tcgccgtgat cggaagtata aatgacccac gtattttcac gttgctctgg
    13861 cgttaaggca ttgatgaccc gtccgatttg gtcatcaaca aagtcattac aggcaaaata
    13921 gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct gcgcccataa
    13981 gcggtgatgt tccggtttgt tcgccaggcc atcctgagct ttctcgccca gatcgtagta
    14041 aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat gcggctcatc
    14101 atacgaaacc accatcagga agggttcctc ggcgcgcgcg ggctgttgca gaaaatctac
    14161 tgcccgattg ctgatgcggt gcgcccaggt gaaggtttcg tcgatatggt tcgcctgtaa
    14221 atcctcaacg ctgtttaggc cattgcgcca caggctgatc tctttttccg tcagttcgct
    14281 aaggtagttc gccccatcga accagtaatc agcgtcccac tccggcggac actcgccagt
    14341 gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt gatagccggc
    14401 atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt tgttggtcca
    14461 cgggccggac tggttagcgt agataccggt aaatagtccg gcgcgtgcag gcgtacaaac
    14521 cggtgaacag gtgtaggcgg aattaaagcg aataccttcc gccgccagac tatcaatatt
    14581 ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct gggtatcggt
    14641 catgatgaac agaaaattgg ggcgtttcat aggctatcct tcgcgtcggg agtaaaggtt
    14701 acggctgggt cgtgacgatg ccgccagctg tcaaaaatca ggtagacaat cactacggcg
    14761 gcaatgaaat aactgatcat cgctaaccag cgcgtaccgt agccgccgaa ttcagccagc
    14821 ccggcataga cgccaatcat cgcgaacagg atgccaattg acgcgatctt gacgtttctc
    14881 cacggtttca tgtccaccgc aaacgcatct ttgaaggtga acggcgtggc gcgcggtttg
    14941 ataaaaccga tcaccagcat cacgaccacg ttgatgcaga acgtacaggc cagcacatag
    15001 aggaaatgga agtcgaactt caccagatag ttgatggtga tgtagctgat tatgccaatc
    15061 cccatcgcca cttttgccgc cagcgccggg atgcgcggga agaaaaagcc cataatgatg
    15121 atggtaacca gcggcacgtt gtaaatgccg ttgagctgtt tcatccagct atacagcccc
    15181 tgcggcgcgt tggcgatcca cggcgcgacc agcaccgaaa cgatagcgat aaagaaacca
    15241 aatttgcgcc cgacggtgac cagctgctgc ggctcggcat tctggttaat gatgcgacgg
    15301 taaataccca tactgaataa cgtactggcg ctattcagaa agccgttgaa ggtactgatc
    15361 accgcaccaa ataacaccgc gccgaagaaa cccaccattg gcactggcag aacgttattg
    15421 accagcgtcg ggtaggccat gtcggctttc ggtaaatcct gatacagatg aaacgcaatc
    15481 aaccctggca gcaccagtac cagcgggtcg agcattttca gcaccgccgt taacagcgcc
    15541 cccttttgcc cttccgccag gctttttgac gccagcgtgc gttgcacgat gccctgattt
    15601 gtacaccagt aaaaggtgtt caccagaatc aaaccggtaa atgccgcgcc aatcggcaag
    15661 ggatcggtcg ggccaccgat tgagtttaat ttctcggcgt gaacggtggt gagttgctca
    15721 atgccctgca taaagctgcc cttgcccatc gcgatcaggc caaataccgg caccatcaac
    15781 ccgccgataa ccagcccaat accgttgatg gagtccgcca ctgccattgc gcgcagtccg
    15841 ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa ttaccagtag ccagatagcc
    15901 gcaccgtgag aaatctgtag cgattccccg acgtgaaaca ggctgttgag cgccaacgcg
    15961 ccggagtaga gaacaatcgg cagaaagcag acgccggtgg caatgaggaa gcagaagtcg
    16021 atgataatac gcgtcgtttt atcataacgt tcctccagaa aatcggggat ggtggcaatg
    16081 ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga agatcagcgt caccgctgaa
    16141 gtcacttccc agcccatcac cgacatgccg cttttgtagg cctgcccgga aaggccgacc
    16201 agttgttccg tggaaaggtt ggttagcatt aacgaagcgg caataaccgg cgcttttagt
    16261 gaacgtccgg caagaaaata gccttgttgc gatccggtat ctgttttgcg gaccttccac
    16321 caggtgatca ccgccaccag cagcgtaaaa ccgacaaaac tcaagatttg taacgaattc
    16381 atctcatagc ccttatttat gtttatgtcc cggacgcatg aatataggga gaggtaaaat
    16441 gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc gtatccctga acggattgtt
    16501 ttctgttaag ttcaggttgt gagcataatt cgccggaggg atattaaaat gaatggaaaa
    16561 ttgcaaagtt cagatgtaaa aaacgaaact ccgtacaata ttcccttatt gattaacgaa
    16621 aatgtgatct ccagcggaat ttctctgatc tcgctctggc atacctacgc cgacgagcat
    16681 taccgggtga tctggccgcg ggacaagaag aaaccgctta tcgccaactc atgggttgcg
    16741 gtgtataccg tacaaggatg tgggaaaatt cttttaaaga atggcgaaca aataacactg
    16801 catggcaact gtattatatt cttaaagcca atggatattc actcttatca ctgtgaaggt
    16861 ttagtctggg aacagtactg gatggaattt acccccacca gtatgatgga tattcccgtt
    16921 ggtcagcaaa gcgttattta taatggcgaa atttataatc aggaactcac cgaagttgct
    16981 gagttaataa cttcaccaga agcaataaaa aataatctgg cagtcgcttt tctgacgaaa
    17041 attatttatc agtggatttg tcttatgtac gcagatggta aaaaagatcc acaacggcgg
    17101 caaattgaaa aattaattgc cactttacat gccagtctgc aacaacgctg gagcgtagct
    17161 gatatggctg ccacgatccc ctgtagcgaa gcctggttgc gtcgtctgtt tttacgctat
    17221 accggcaaga cgccgaaaga atattacctc gatgcgcgtc tggatctggc gctatcgcta
    17281 ttaaagcaac aaggaaactc ggttggcgaa gtcgctgata cgctcaactt cttcgactcc
    17341 tttcatttca gcaaagcctt taaacataaa tttggttatg cgccgtcagc cgtgctaaag
    17401 aatacggacc agcacccaac ggatgccagt ccacacaatt aacgtaactc tggccagtac
    17461 gctttgttgg cctccagcaa ctcatccaga atagctttgg cgaccgaggc gctcggtacc
    17521 gttttcgaca acgtaatcgc ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc
    17581 acgaccagtt tttccaccgc cacttgctga ctcattaacc ctttctgaaa ctgcgggata
    17641 tcgccgacca ccagtggttc aggccgtagt agcgcacgcg catctgtttg cgatcttgca
    17701 gcccaacaat ttgcgccatc cggctttcaa taccgattgg catgtcacag atgttgagga
    17761 ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac aattgctgcc gggttggagt
    17821 agttgagcat ccaggcgttt ggtgaatatt tttccatata atccaccagt tccaggacgc
    17881 cgccaatgga acgcatgccg tacgctattc cgccgggtcc gcaagtttcc tgaccaacaa
    17941 caccgtggcg cagtgggatt ttttcatcca gttcgcgcat cgggtacttg ccgacgcgga
    18001 tgtgcgccat gacaaaatca acgtcgctga acgctacttc aggatcggtg gtgtaactaa
    18061 acgcaatgtc cggcgctttt tctttaagga tgactttaca ggcttcggca atcacttcct
    18121 gccgcgcgcc atcgttatca taaaatttca gtgcacgaag cgggaaacgg tcctgattcg
    18181 ccaggagcat caacacgatg cctggcgtaa aggtgcttcc accgcctgcg acaaccactg
    18241 agaatttggt cattatactg cctccgtaat ggcaacattt tctgctgatt gatgagaatt
    18301 aattaagcta tcgagctgtt cacgcaattg ggatacatgc agaccaatga tcacctgaat
    18361 ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag
    18421 cgtttgtgac atgtcatgca gtgcaatgcg tagacgcgtc gcgcaattgt taatgctgga
    18481 gatattgccg accccgccca gggcttgcag aataccggca gcctgatcca gctctttttt
    18541 tggctctgcc gcggtggttt ggcctcgcga ggctttgtat tcggcttttg agtagagttt
    18601 cacttccgca tcttcacgtc ccggcgtgca catgttgaac tgcagaatca gggtgcggaa
    18661 aaccacgaag tacagcaggg taaagcacaa cccaatggcg atttgggtca gcatcatatc
    18721 cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc
    18781 gcccatgttg cccaccacac caaagagata catcacggtc gacattgagg ccgccagcac
    18841 ggcgtgtacc gcaaacagca acggtgaaat gaacaggaag gtaaattcca gcggttcggt
    18901 aattcccacc agcatggcgg ttaaggttgc gggaatcagc aagcccgcca cttttacccg
    18961 attttccggt gcggcagtga agtacatcgc taaagaaatg cccacggcac caaagatttt
    19021 tgagttaccg tgcagggcaa aaccgccttc cgggaacaac gatttcagcg gctcggcgct
    19081 cagactgaac tcttgcagat gctgcgccca gtacatctga atgccgcctt caacagctgc
    19141 cggaccaaag ataaacggtc cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg
    19201 ttcgaggaag gtgtaaaccc acaccccaag cgcacccgcc gaacgcagga acgcttgcag
    19261 agattcaatc cccatttgta cttttggcca gccgagcaac gtcagccagg cacaggggat
    19321 catcaccagg aaggcgataa tcaccacata agacgtcccc tggaaaatgc cgagaaaaac
    19381 cggcagtttt ttatcgaaca gacggttatg cagcgccgtc acaatgccgg aaatgataat
    19441 tgcgccgata atgctggtat cgagggtttt aatcccggcc atcattgtca gaccgctacc
    19501 tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc
    19561 gttgatgaaa tagttccagg tcaggaaact caccatcacc gccagacaag cacgcccctg
    19621 cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca tattacggaa
    19681 caccgtccaa ccgccctctt caataatgtg tacgatttgc gcgaataaac tgttcggatc
    19741 ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag atggcaagac ccaccacaat
    19801 cccggcgaag ggaaacagca gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg
    19861 actgagcatt tccacatcct cttttctcaa ttctgtaagt aggaagcctg attggttatg
    19921 cggcaggcct ggagcgagga taggaagatg cgatcggcag aacatgacga cccaatggga
    19981 ttcgtgatcg ctttcatgct tttcgttggg cggtagagat ctacgttttc gcgaaaaagt
    20041 agacatctgt gagcacaggt acacagcatc gaacacatct ttaaaaaaaa gatgtttttt
    20101 caatcgatta agcagaactt gtgggcgcat tacccgtgct tgcaggcaaa aaagagatct
    20161 agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt ccgcagattt
    20221 cacgctaaat agccttcttc ccggtgaaaa aaagttggcg gaagaatttg cggtatcgcg
    20281 gatgaccatc cgtaaagcca ttgacctgct ggtagcgtgg ggattggtgg tccgccgcca
    20341 cggcagcggc acttacctgg tgcgcaaaga tgtactgcat caaaccgcca gcctgaccgg
    20401 gctggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc tgatttttga
    20461 aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca acgagcagat
    20521 ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg aagacagcta
    20581 tatgccggta aaactgttcc gtaatctttc gctgcaacat ctggaagggt cgaagtttga
    20641 atatattgaa caagagtgcg ggattttgat tggcggtaat tatgaaagcc tgacgccagt
    20701 gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg
    20761 gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag tgatgttcag
    20821 aaatgccagc gaataccagg tggagtacca tttacggcga ctccacccgg aaaagagtta
    20881 accgatactc cagaagagca ccgccagtaa ttggggggtg ataatgcgca ggaacatcac
    20941 taacggatag acagtggcgt aagagagcgc cgccgcaccg ctggttggat gaagattatt
    21001 agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc acatggtcag
    21061 gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg gaacggcggt
    21121 gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg tattcacaaa
    21181 atctccacca gatttcagac caacgaccga gaggaacagc acgatcccca gctcccgcag
    21241 cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc cgatacgccc
    21301 gaggatcaac gccataatca gcggaccgcc cgccagcccc agtttcaacg cggccgggaa
    21361 cccaggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc caataaacac
    21421 tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat tggcaacggc
    21481 atcaattgcg gacggacgcc ccaccagatt gaggatatcg ccgaactgca ggctgatatc
    21541 gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa cgtcatagcg
    21601 ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg tgaccaccac
    21661 acgctcgacg cgcaaatcag tgcctttcgt ggacagcgaa gtatcgacct cctgaccaat
    21721 caccagttgc gcattatgta aatccgctgg ctgccccacc agatgcagca aatcgcccag
    21781 ttggataatg gtatctggcg aaggaacttt tagggtttct tcgcgtttca gacgcgagca
    21841 gataattttg tcgccgttga gaatcggtac atctttaatg gctaaatcat gcaggttagg
    21901 gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc gtgaagactc
    21961 gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca tccacatggt
    22021 gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc ccatctgatc
    22081 gaccatttcc attggtgtac ccaagtcgcg caaaatctgc tgccctgccc ccagcgctgg
    22141 cgtattggta accgcaccgg agaaaatccc cagcactacc ggcagtggaa tatcaaacag
    22201 tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag caaacaggtt
    22261 gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct gaatcccgat
    22321 agtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat cgctacttaa
    22381 tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc
    22441 aatacctaat cctatgccgc gaaatttgac attgccgata aacaaaccga cgactgccac
    22501 caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct cttgagataa
    22561 catttttaat tgctacggat tatggctgaa tcggtgaagc agatttgcct tatagcgcac
    22621 attatcacgg gaaagaacgt gccgaaatat cttaaacagt cgaaatttaa cgcattgttg
    22681 agtaagtaag acaaaaaaag ccccaccaga atggcggggc aaagagaata gctagttagc
    22741 tatttaacgc gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat
    22801 tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag acttccatat
    22861 tttcagccag cgtaaagctc aggctaaatg gctgattcat aagcccttga tgcagccatt
    22921 ttttctcttc ttttggctgc tccggcgtgc cttttacgct caggcgcgta ccttccagtt
    22981 gaatctctaa atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat
    23041 cgtcgctttt ctcaatgttg tacggcggga agctctggct ttcaccggcg ttttgcagtg
    23101 cgttggccag tttgtcaaaa ccgatccatt gacgcatcag tggggataaa tcgaagttac
    23161 gcatagtcat ttctccttct aagaagcgag taagtacctg caaatccgaa gattcgcata
    23221 tgctccctaa cggcgagcat ggagatgtca ggccgcgcca ggcggcctta gggaattagt
    23281 tgatttcgat acggcgcggt tttttcgctt ccggaatcac gcgttcgaga tcgatataca
    23341 gcaaaccatt taccaggtta gcgccacgaa catgaatgtt ctcagctaac tggaatttgc
    23401 gttcaaagtt gcgttcagcg atgccctggt acagataggt gcgctctttt tgttcgtcgg
    23461 cgtgagcacc tttcaccact agcagattat cctgggcggt aatttccagt tcgctctcag
    23521 caaaaccagc cacagcgata gcaatgcggt aatggttttc gtctaccagt tcaacgttat
    23581 acggagggta gccgccatta ctctggctct ggttgttttc taagtggtta aacaaacggt
    23641 caaatccaat agcagaacgg taaagcgggg ataaatcaaa gttacgcata atcaatagct
    23701 cctgaaatca gcgagaatgt aagaccttcc acaatggaca ggtcaggtag ccagaacacc
    23761 catcaggcgc gtcctcatcg gctacgatgt aaaaatgggt ctggaaatga acttttcaag
    23821 ccttatcagc ggactttttt ttgcagttta tggtctattg catagactga gggggcagca
    23881 cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa tgatgatgtt attttcagca
    23941 agggatcgct ataactcatc aggccaacac ccacgatcaa cagatgaatt tattatgata
    24001 agaaatgtgt tgttagcgtt catgatatgc agcggaatga cattactcgg aggatgctcc
    24061 agcgtgatgt cccataccgg agggaaagaa ggaacgtatc ccggcacccg cgccagcgca
    24121 accatgattg gcgacgatga aaccaactgg ggcactaagt cgctggcgat tctcgatatg
    24181 ccgtttaccg ctgtgatgga cacgctttta ctgccgtggg atgtgttccg caaagacagc
    24241 tcggtgcgct cacgcgttga aaaaagcgaa gccaacgcgc aagccaccaa tgcggttatt
    24301 ccaccggcca gaatgcctga taactgattt agcgatcagt ttcggtgatc cacagctggc
    24361 caccttccat ccacgccagc cattgaccat ccggcgagaa gacctcggcg tccgcagaag
    24421 gtggattggc gtggttttcg gttaaatact caacctcgcc gctttgcgca tgggcacaag
    24481 caattcgatt atccagcaca aagcccaacc attctcctga cggatgccag ttaaatgcag
    24541 actgaatatc cgttttgtta tgggttaact ggcgcggctc gccgccctgt ggcgagataa
    24601 gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc gtaccctgcg
    24661 gattacagcg cacccagtgg cgcgggacgt tgactaaccc cggataagcc cgatggtggg
    24721 taaaggttaa acgtcgctgc acgacgccgc gcggtggcgc gggcagggtg gtttccgttc
    24781 cacttaacgg cgcatcacct gccgctttcc agccagcttc atcttgcggt aactcaacga
    24841 taaacagctc cggcactttc tcgccctttg gcgaaagtgt gtcgccaata aacgccagcg
    24901 cgtgatttcc tacccatcct tcttcataag cacgattgat ttcatcgctg ccaggctgcg
    24961 gcgtgggcgt ggttttactc accagcacgc accagtggct accgctgtat tcacgcggat
    25021 gctgcttttg tacgttgacc gggccaaacg gcgcagcaac accgacgttt cgcaaatcca
    25081 gcgccggatc gagttcatgc attacatggt cgttataggt aaagctcacc ctttcaccgt
    25141 tcgggctaaa gacatgcaca tggctgccgc cgcgcagcgc tcctggcata tacggcgcgg
    25201 taatatccat tgcatcgaga ttgctcacct tgccgccttc agcaatgact ccgcgtcgat
    25261 gatggaaatc gtaatgccat gtttcatcag gattttccgg gccatggatg aaaacatatt
    25321 tctctgactt cggatgaacg gtcaccacgc cgacgtgtgc gccctgtgac gcgcgataga
    25381 taacctcgac ctcgccggta tggatattca cacgctcaat ggtctcgccg gtaaacgacg
    25441 ccccagaagg acgcacgtca aataccagcc actggctgtc gggcgtccag gtattggtat
    25501 tggtgagcag gtgattacgg ggagcaaagg ttatctgttt catggcgcgg tcctgataca
    25561 aaacgggtcc cgccatcata cttcacaagg acttcacttt cagacaatag tcttttgcca
    25621 tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt ggcctcggcc
    25681 cggcaggatc ggcgttggcg cgaaaattag caggcaaaat gcaggtgatc gcgctggata
    25741 aaaagcacca gtgtggtact gaaggtttca gcaaaccttg cggcggtctg ctggcaccgg
    25801 acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtg atcgccaacc
    25861 cgcagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    25921 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    25981 ccagcgttga gatttatcac gacagcctgt gtcgaaaaat ctggcgtgag gatgataaat
    26041 ggcatgtcat ttttcgtgca gacggctggg agcaacatat tactgcccgc tatctggtcg
    26101 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    26161 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    26221 tctttgataa ttcgataact aactgttatt catggagtat cagcaaagac ggttatttta
    26281 tctttggcgg tgcttatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    26341 aaatgagcgc ctttcagttc cagtttggta aggcggtgaa aagcgaaaaa tgcacggtgc
    26401 tgtttccctc acgctggcag gattttgtct gcggtaagga caacgccttt ctgattggcg
    26461 aagcggcagg atttatcagc gccagttcgc tggaggggat tagctatgcg ctggatagcg
    26521 cagacattct gcgttcggtg ttactgaagc agccagagaa gctcaatacc gcttactggc
    26581 gcgccacccg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    26641 ccgcaccggc tttaagaaag cggattatgc gcagtggtat ggcgcatatt ccacagttga
    26701 aagattagcc aacgcgcttc acatcgccca ccagcaggat gtaagagagc gcgccaatca
    26761 acgcgacggc ggagatataa accagtgcag gtgcgaaacc gtaaccctgc gccaggtacc
    26821 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccgccgg
    26881 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    26941 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    27001 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    27061 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    27121 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    27181 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgtagaag
    27241 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    27301 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    27361 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    27421 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    27481 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    27541 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggga
    27601 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    27661 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccctggaat aaagtcgcca
    27721 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    27781 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    27841 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaggttag
    27901 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    27961 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    28021 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    28081 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    28141 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    28201 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    28261 catactgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    28321 aatgcccata ctttgttcct gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    28381 agctgccagt gcaatcggtc cgagcggaca gtgcggcgca agggtcacat cataggcttc
    28441 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    28501 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    28561 ctcacctgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    28621 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    28681 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    28741 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    28801 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    28861 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    28921 tttaattttg tcgcgaacca ggccgcccat cagttgccag accggcgcat tcagcacttt
    28981 gcctttgata tcccataacg cctggtcgat cccggcaatg gcgctcatca ggattggacc
    29041 gccacgataa aatccggcgc gatacatcac ttgccataag tcattgatgc gcgaaggatc
    29101 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    29161 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    29221 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    29281 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga
    29341 tagagatcgc tgcctaagcc cgccccagca caacctgcgt ctatccactg cgccaggttt
    29401 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg atggcaatac cgcttttaac
    29461 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    29521 gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcagac ggtcatgccg
    29581 tagcccaccg cacggcggat cacttcacta tggatattgg gcgtaacgat gagctgacaa
    29641 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    29701 gccttgtcgc catacgcatc aacgatggcg ggaatgcttt gctcccattg tggggaattc
    29761 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    29821 tcgtcgggct taatgccgcg caaaatggcg atcagcggaa gtttagtttg ccactgcatg
    29881 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg tcacgtcgca
    29941 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    30001 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    30061 accagagaga aattcgctga cctgttcgcg gggaagtgtt cccagcacat gcgaggcgcg
    30121 aacttcaaaa agctgcggca atatggcggg cgcattaagg ccgcgctcaa ggccagccgc
    30181 gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaatcaatg agtgatttaa
    30241 cagtaaatga tgtaattcac cggtcatcac ggtgcgaaaa tcgttgattt gctggctatc
    30301 ggcctgcacc catttgcaat gcgtaccggg cataacataa agagaggaag gagccagagt
    30361 tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat cgtcatgaga
    30421 gacgcataat ccgggaataa tccagatatt gtcgccaaca gacgttaatt gttcgccaat
    30481 agacgaaaaa cgggcaggaa cagataaata cggagcaact ttccagccga cattgctgcc
    30541 gaccattcct gccattacca ctggcgtatt ctcttcacgc cagtcggtcg tgacttctgc
    30601 taacaccgca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg attgcctgct
    30661 ctccaggcag tggtcgccct gataaagcca ggcgcgaaga ttggtcgatc cccagtcaat
    30721 tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct ggcgatcatg
    30781 gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc gtattgcatc gaacagcgcc
    30841 ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt tcgttcaaaa
    30901 accgcccgct gcaacgaact gatcgcaatg ctaagttgct gtaacaccgg gttatgtacc
    30961 gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc gcggtcctga
    31021 ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct ggaagtcgcg
    31081 cgctctgctg cccaacgggc aatcgccggt tccaccagat ttcgcacttc gctcatggca
    31141 ctgataagcc gtgggtcgta gtcgttttcc agcacccatt gcagtacgtc agtgtcgagg
    31201 taattccact ggttacgcgg tgccacaaac gcgccgcgat aacgtttcat ttcaatcagc
    31261 cgtttcgcca tcaacgaacg gaacacctca cggatgatgt tgcgcgaggt tgcaaactct
    31321 tcacagagct ccgcctcagc cggaagtggc gagccaggta cgtatttgcc gtgaacaatc
    31381 tgtttaccca gcgtaatgac aatgcgatcg gttttattga gagtcatgga gagtccttgt
    31441 gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa ctttgtagta
    31501 ccagcgtgat gacgttcgcg tttgccgtgt gcgtaatgta gtacaaactt atattgttgt
    31561 actacaattt agatcacaaa aagaacaatg cataaaaaaa tgacatgcgt cgggcagaaa
    31621 tctgaaaagg gatatcaggc gctaaacagg agggaaagaa gagtatgctt tcaacggctt
    31681 agctactcgt ttaaaggatt aatcatgaag ttgaatttta agggattttt taaggctgcc
    31741 ggtttattcc cactggcgct gatgctttca ggctgtatct cgtatgctct ggtttcccat
    31801 accgcaaagg gtagttcagg aaagtatcaa tcgcagtcag acaccatcac tgggctatcg
    31861 caggcaaaag atagtaatgg aacaaaaggc tatgtttttg taggggaatc gctggattac
    31921 cttatcactg atggtgccga tgacatcgtt aagatgctca atgatccagc acttaaccgg
    31981 cacaatattc aggttgccga tgacgcaaga tttgttttaa atgcggggaa aaagaaattt
    32041 accggcacaa tatcgcttta ctaccactgg aataacgaag aagaaaaggc actggcaacg
    32101 cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt cactggaaaa cctaaaaggc
    32161 acaatccatg agaaaaataa aaacatggat tactcaaagg tgatggcgtt ctatcatccg
    32221 tttaaagtgc gattttatga atactattca cccagaggca ttccggatgg tgtttccgca
    32281 gcattactgc cagtgactgt tacgctggac atcattactg caccgctgca atttctggtt
    32341 gtatatgcag taaaccaata atcagtaagc gggcaaacgc gtttatgctg tttgcccgtc
    32401 cactgattaa ttcagcacat acttctcaat agcaaacgcc acgccatctt caaggttgga
    32461 tttggtgaca aagttcgcca cttctttcac cgaaggaata gcgttatcca tcgccacacc
    32521 gacgcctgca tattcaatca ttgcgatgtc gttttcctga tcgccaatcg ccatgatttc
    32581 ttccggttta atacctaaca cgtcggccag tgatttcacc cccgtacctt tgttaacgcg
    32641 tttatcgagg atttcgagga agtacggcgc acttttcagc acggtatatt tctctttcac
    32701 ttcctgcgga atacgcgcga tagcctggtc gaggatggcg ggttcatcaa tcatcatcac
    32761 tttcaggaac tgggtattgg ggtccatttt ctccgcttcg cagaacacca gcggaattgt
    32821 ggcaacgaag gattcatgca ccgtgtagta gctgatatca cggttggcgg tgtacagcgt
    32881 ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga gagagttttt ccaggaaacg
    32941 gtagtcgtca tagctgagag cggtttgcgc cacggtgcta ccatcagcgg ccttctgtac
    33001 cagcgcgccg ttataagtaa tgcagtagtc gcccggcagt tccatatgca gctctttcag
    33061 gtaattgtga acacctgcat aagggcgacc cgtcgttagc acgacattca cgccacgggc
    33121 gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg gtgtgatcgg gtagcagaag
    33181 ggtgccatcc atatcgatag caatgagttt gatagccatg agttccccag gtagattagt
    33241 tcctgaccca tgctaacgcg attccgctca aaaatcagta caacacccga gggaaaaggg
    33301 ggatgcaacg cgggttgcat gctccccatt tgcttagcgg aagagtttcc ctttcagcag
    33361 ttccatgcct gcggaaagca gatcgttatt ggcttgtgga gacacttcac cttgcggtga
    33421 gagcgcatca ataatcttcg gcaattgttc tgccagtaaa ctggaagctg tactggtatc
    33481 cacgccaagt ttttgtccaa gatcggacac cgcatttgtg ccgagcgccg attccagttg
    33541 ctcgccacta accgattgat tgccctgttg attactcagc caggttgaga gaatggcccc
    33601 taagccgcca ctttgcagtt tttccagcag cacctgaatg ccgccctgct cctcaaccca
    33661 acttaaaata gcctgatatt tccccgcatc gcctttcaga aaggcaccga caacttcatc
    33721 aaaaagcccc atgataatca cctgtaaagc gttacgtgtt gacccaaaaa gtatagattt
    33781 gcgggatgat aattgcggat tgcagaaata aaaagggcgg agatgatctc cgcccttttc
    33841 ttatagcttc ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgaaaat
    33901 tcaatacatt gcaagatttt cgtaggcctg ataagcgtag cgcatcaggc acgctcgcat
    33961 ggttagcgcc attaaatatc gatattcgcc gctttcaggg cgttctcttc aataaacgca
    34021 cggcgcggtt caacggcgtc gcccatcagc gtggtgaaca actggtcagc ggcaatcgca
    34081 tccttaacgg taacgcgcag catacgacgg ctttccgggt ccatggtggt ttcccacagc
    34141 tgttccgggt tcatctcgcc cagaccttta taacgctgga tggagaggcc gcgacgggac
    34201 tctttcacca gccagtccag cgcctgctcg aagctggcta ccggctgacg acgctcgcca
    34261 cgttcgataa acgcatcttc ttccagcaag ccacgcagtt tctcacccag cgtgcagata
    34321 cgacgatatt cgccaccggt gataaactcg tgatccagcg gatagtcagt atccacaccg
    34381 tgggtacgca cgcgaacaat cggctcgaac aggttttgtt cggcattggt gtggacatca
    34441 aacttccact ggctgccgtg ctgttctttg tcgttcagtt cgctgaccag cgcgttcacc
    34501 cagcgggtaa cggtctgctc atcagagagg tcggcttccg tcagcgtcgg ctgatagata
    34561 agctctttca gcattgcttt cggataacga cgctccatgc ggttgatcat tttctgcgtc
    34621 gcgttgtact cagacaccaa tttctctaac gcttcgccag ccaatgccgg tgcgctggcg
    34681 ttggtgtgca gcgttgcgcc atccagcgcg atagagatct ggtactgatc catcgcttcg
    34741 tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt tgtacagcgg cggctgagcg
    34801 atgtagacgt gaccgcgttc aacgatttcc ggcatctgac gatagaagaa ggtcaacagc
    34861 agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca tgatgatgat gctgtgataa
    34921 cgcagtttgt ccgggttgta ctcgtcacga ccgataccac agccaagcgc ggtgataagc
    34981 gtcgccactt cctgagaaga gagcatctta tcgaagcgcg ctttctcgac gttgaggatt
    35041 ttacccttca gcggcagaat cgcctggttc ttgcggttac gcccctgctt cgcagagccg
    35101 cccgcggagt ccccttccac caagtacagt tcggaaagcg ccggatcgcg ttcctggcag
    35161 tctgccagtt tgcccggcag gcccgctaag tcgagcgcac ctttacggcg ggtcatttca
    35221 cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt
    35281 ttcgcgtcgg ttgggttttc cagcaggtat tctgccagca gttcgttcat ctgctgttca
    35341 accgctgatt tcacctcaga agaaaccagt ttgtctttgg tctgggagga gaatttcggg
    35401 tccggcactt tcacggaaac gaccgcaatc aggccttcac gcgcatcgtc accggtggcg
    35461 ctaactttgg cttttttgct gtagccttct ttgtccatgt aggcgttcag ggtacgggtc
    35521 atcgccgcac ggaagcctgc caggtgagta ccgccgtcac gctgcggaat gttgttggta
    35581 aagcagtaga tgttttcctg gaagccatcg ttccactgca acgccacttc gacgccaata
    35641 ccgtcttttt cagtggagaa gtagaagata ttcgggtgga tcggcgtttt gttcttgttc
    35701 agatattcaa cgaacgcctt gatgccgcct tcatagtgga agtggtcttc tttgccgtcg
    35761 cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt
    35821 ttcgccagaa tttcatattc gaactcggtc acattggtga aagtttcgag gctaggccag
    35881 aaacgcacca tggtgccggt tttttcagtc tcgccggtaa ccgccagcgg ggcctgcggt
    35941 acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctggataac cagctccagt
    36001 ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta
    36061 taggagttat cgtcgaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc
    36121 gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag
    36181 acagagttgt cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg
    36241 tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg
    36301 tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata
    36361 ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc
    36421 cgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac
    36481 gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc
    36541 aagcaaccca cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca
    36601 ccgccgcccg ctttcacggg tgaggaactc tccttgcgcc agacgtaagg cgcacatcaa
    36661 cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg
    36721 taagtccgct ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt
    36781 acgttccagc acctcagcat attctgtctc tttctcccag ccgcgctgga aagagaaagt
    36841 cagagaaaac tcagggagaa attgcttaca ggtatcggcc atatcggccg cgataccggc
    36901 gctatactcc gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca
    36961 cgggcgtagc tgttcgtaac gtgtcacctg gcgcagcgcc gcattgcgct gcttgagcaa
    37021 tcgcttgaga ttgctccagg cggtgaaaaa tccgggttcg ttgtgaaagc atccccagtc
    37081 gaggaatgct cttctgtatt tggggccgcc gttgagtaaa gtaaaccctt ctggcgttat
    37141 cagctgcatt ggcatcaggt gcgccagttc cgcgacttta tgcccgtcag taccgtcgat
    37201 gcggactttg ctgtcgccct gtttgtcctt ggttaagcca atcgccgtct cgcgctcttc
    37261 gccctgtaat cgcccatgga gaacaaatgc ctcctgctca tggcgaatga cgcgaccaat
    37321 ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag atggcttcca gcacgctggt
    37381 tttgccactg ccgttggcac ctaccagaaa gttaaagccg ggagataaag cgagatccgc
    37441 ggtttcaatg ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcatt
    37501 ggcatgacaa cataggcagc cgactgtgat gcggcatctt caatctgcac gctggaaacc
    37561 gaatcggtca gcatcatgcg gacgttttcg catttcagcg cgttcagaac atccagcaca
    37621 tagctgacgt tgaagccgat ttccatctcc gcaccgctat aggtaacgtc gaggatctct
    37681 tccgcttctt cctgttccgg gttgttggcg gtgattttca gctggttttc gctgacatac
    37741 aggcgcacgc cgcggaattt ctcgttagag agaattgccg cacgggcaaa cgcctgcttg
    37801 agcagatcgc agccagcttc cagatgtttg tccggattct tcggcagaac gcggcggtaa
    37861 tccgggaagc gaccatccac cagtttggag gtgaagataa agtcgccaac gtgggcgcga
    37921 atattgttgc tgccaatctg cacgcgcagc ggattgtcgc cgccgtcgag catacgcatc
    37981 agttcaatca cgcctttacg cggtacgatc accgaatggc ttggcaaaga ttgaccaatt
    38041 ggcattgaac agaccgccag acggtggccg tcggttgcca cggtgcgcag ttcttcacct
    38101 tcggtttcaa acagcatacc atttaagtaa tagcgaacgt cctgatgcgc catcgaaaac
    38161 tgggtcgctt caatcagacg cttcatcgtt gcctgcggca gggtaaattc gacttcactc
    38221 tgccagtcat cgaggttcgg gaaatccgcc gctggcaggg tagacagcga aaaacggcta
    38281 cgcccggagc gtaccagcat ccgttcacct tccagctgca cggcaatttc cgcgccttca
    38341 ggcagaccac ggcagatatc aaagaatttg cgcgccggaa cggtcgtcgc tcctggctcg
    38401 tgtggctgaa ccagcgcaac acgtgccacc atttccatct cgagatcagt accggtcagc
    38461 gacaacgtac cgtcagcaac ctgtaacagc agattaccga gaatcggtag cgtaggacga
    38521 ccacctaacg gaccgctcac ctgttgtagc ggttttaata aatgctcacg ttctacggta
    38581 aatttcatag gtttacgatg acaatgttct gattaaattt gaaaaatctt ctttgatatc
    38641 gtggctctct tcacgcaact gctcgatctt acggcaggca tgaagcaccg tcgtgtggtc
    38701 acgaccacca aacgcatcgc caatctccgg cagactgtgg ttagtcagct ctttcgccag
    38761 cgccatcgcc atctggcgcg gacgcgccac cgagcgggat cgacgcttgg aaaggagatc
    38821 cgcgactttg atcttgtagt actccgccac cgtcttctga atattgtcga tggtgaccag
    38881 tttttcctgc aatgccagca agtcgcgcag cgcctcacgc acgaagtcga tggtgatcgc
    38941 ccgtccggta aagttggcat tggcaatgac gcggttcagc gccccttcca gctcacgtac
    39001 gttagatcgt agacgcttgg cgataaagaa cgccacttcg cccggcaaac gaatgtcgtt
    39061 ttcgtcggcc tttttcatca ggatcgccac acgggtttcc agctctggcg gttcgattgc
    39121 cacagtcagt ccccaaccga agcgggattt caaacgatcc tcaacgccgt tgatctcttt
    39181 cggatagcga tccgaggtga gaatgatctg ttgattacct tccagcaggg cgttgaaggt
    39241 atggaaaaac tcttcctgag atcgttcttt attagcaaaa aactgaatat cgtcgatcag
    39301 cagtgcatct acggaacggt agtagcgttt aaactcttcg atcgcgttgt tttgcagggc
    39361 tttaaccatg tcctgaacaa agcgctcgga gtgcatataa accactttgg cattcggctt
    39421 gcgcgccata atgccgttac ccaccgcatg cagcaggtga gttttaccca gacccgtgcc
    39481 gccataaagg aacaacgggt tataggcacc gccagggtta tccgccacct ggcgagccgc
    39541 cgcgcgcgcc agttggttag atttaccttc aacgaagtta tcaaacgtgt gtttgacgtt
    39601 tacgttagaa cgataggtcg gttctgccgg ggccgggatg ttatcccaac ctgagcgcgt
    39661 agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt gcaggggccg cgacgttgct
    39721 cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg ccgacttcaa aacgcagctg
    39781 tggggcatcc gctccgcaga aactggttag cagtccattg atattattaa ggtacttgtc
    39841 ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg gccagcgtgt tatcgctcag
    39901 ttccgcctgc aatgggcgta tccacatact gaattctgtg gctggtaact catcctgcaa
    39961 tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg gactccactc gaacaaaagt
    40021 cgataatgac taaggctgaa acattcatga ttgttgacgt acgtcgaaaa gaccctgctt
    40081 gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg atcaagatcc tgcaaaacga
    40141 tcgggaccgc ggatcatagc ctaaactgcg caagagatct tctgtttctc acagattttc
    40201 ccgatttatc cacaggactt tccagacctc gctaagtgta aacgatcctg ccgcgaggcg
    40261 ggcacgattt acgccgcata ttggaaaatt taatgaccat agacaaaaat tggcttaatc
    40321 gatctaataa agatccagga cgatccttgc gctttaccca tcagcccgta taatcctcca
    40381 cccggcgcgc catgctggtt ttcactggtg tgaggtcgta cattttccct gcgaaaaggt
    40441 gcggaaaagc gcggtaaata aggaaagaga attgactccg gagtgtacaa ttattacaat
    40501 ccggcctctt taatcaccca tggcttcggt gtccatcgtt tcatttttcg gcggatatcc
    40561 aataaagcca ttgaatttat tcaagtttag gtagaaatcg ccatgaaacg cacttttcaa
    40621 ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg ctcgtatggc tactaaaaat
    40681 ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg ctcgtctgac cgtttctaag
    40741 taataaagct aacccctgag tggttaagct cgcatttccc agggagttac gcttgttaac
    40801 tcccagtcaa ttcacattcg tcttccagca gccacaacgg gctggcacgc cgcaaattac
    40861 cattctcggc cgcctgaatt cgctggggca tccccgtatc ggtcttacag tcgccaagaa
    40921 aaacgttcga cgcgcccatg aacgcaatcg gattaaacgt ctgacgcgtg aaagcttccg
    40981 tctgcgccaa catgaactcc cggctatgga tttcgtggtg gtggcgaaaa aaggggttgc
    41041 cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa ttatggcgcc gccactgtcg
    41101 cctggctcgc gggtcctgat agccctcatt cgggtctatc aacgcctgat tagtccgcta
    41161 ctcgggccgc attgtcgttt cactccaacc tgttcaagct acggaattga ggcattgcgc
    41221 aggtttggag tgataaaagg cagttggttg acggtgaaac gcgtattaaa atgccaccct
    41281 ttacaccctg gtggtgacga tcccgtcccg cccggaccat ttgataccag agaacactaa
    41341 cgatggattc gcaacgcaat cttttagtca tcgctttgct gttcgtgtct ttcatgatct
    41401 ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc ccaacagacc acgcagacaa
    41461 cgaccaccgc agcgggtagc gccgccgacc agggcgtacc ggccagtggc caggggaaac
    41521 tgatctcggt taagaccgac gtgcttgatc tgaccatcaa cacccgtggt ggtgatgttg
    41581 agcaagctct gctgcctgct tacccgaaag agctgaactc tacccagccg ttccagctgc
    41641 tggaaacttc accgcagttt atttatcagg cacagagcgg tctgaccggt cgtgatggcc
    41701 cggataaccc ggctaacggc ccgcgtccgc tgtataacgt tgaaaaagac gcttatgtgc
    41761 tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta taccgacgcg gcaggcaaca
    41821 cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc tgtcaacgtc aactacaacg
    41881 tgcagaacgc tggcgagaaa ccgctggaaa tctccacctt tggtcagttg aagcaatcca
    41941 tcactctgcc accgcatctc gataccggaa gcagcaactt cgcactgcac accttccgtg
    42001 gcgcggcgta ctccacgcct gacgagaagt atgagaaata caagttcgat accattgccg
    42061 ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt ggcgatgctg caacagtatt
    42121 tcgcgacggc gtggatcccg cataacgacg gtaccaacaa cttctatacc gctaatctgg
    42181 gtaacggcat cgccgctatc ggctataaat ctcagccggt actggttcag cctggtcaga
    42241 ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat ccaggacaaa atggcagctg
    42301 ttgctccgca cctggatctg accgttgatt acggttggtt gtggttcatc tctcagccgc
    42361 tgttcaaact gctgaaatgg atccatagct ttgtgggtaa ctggggcttc tccattatca
    42421 tcatcacctt tatcgttcgt ggcatcatgt acccgctgac caaagcgcag tacacctcca
    42481 tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat gcgtgagcgt ctgggcgatg
    42541 acaaacagcg tatcagccag gaaatgatgg cgctgtacaa agctgagaag gttaacccgc
    42601 tgggcggctg cttcccgctg ctgatccaga tgccaatctt cctggcgttg tactacatgc
    42661 tgatgggttc cgttgaactg cgtcaggcac cgtttgcact gtggatccac gacctgtcgg
    42721 cacaggaccc gtactacatc ctgccgatcc tgatgggcgt aacgatgttc ttcattcaga
    42781 agatgtcgcc gaccacagtg accgacccga tgcagcagaa gatcatgacc tttatgccgg
    42841 tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct ggtgctgtac tatatcgtca
    42901 gcaacctggt aaccattatt cagcagcagc tgatttaccg tggtctggaa aaacgtggcc
    42961 tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt ttcgctaaaa taagggcggt
    43021 cagttgaccg ccttttttct tttcgtaggg cggataagca ccgcgcatcc gccacacaaa
    43081 gcaacaggaa catcatgagc gataatgaca ctatcgtagc ccaggccacg cctccgggac
    43141 gtggcggtgt tggcatcctg cgcatctccg gcctcaaagc ccgtgaagtt gccgaaaccg
    43201 tgctgggtaa actacctaag ccgcgctacg ccgattattt gccatttaaa gacgccgacg
    43261 gcagcgtgct cgatcagggg attgcgctat ggttccctgg cccgaactcg ttcaccggcg
    43321 aagatgtgct tgaactgcag ggtcatggcg gtccggtgat cctcgacctg ctgttaaaac
    43381 gcattctgac cattcccggc ctgcggattg ctcgccctgg tgagttttcc gaacgcgcgt
    43441 ttctttacga taaactcgac ttagcccagg ctgaggcgat tgccgatctt atcgacgcca
    43501 gctcggaaca ggcggcccgt tcggcactta actcgctgca aggcgcattc tccgcacggg
    43561 ttaatcatct ggtagaagcc ctcacccact tgcgcattta cgtcgaagcg gcaattgatt
    43621 tccctgatga agagatcgat ttcctctccg acggcaaaat tgaagcccag ctcaatgacg
    43681 ttattgccga tcttgatgca gtgcgtgctg aagcacgtca gggtagtttg ttgcgcgaag
    43741 ggatgaaagt ggtgattgcc ggacgtccta acgccggtaa atcgagcctg ttaaacgcgc
    43801 tggctggccg tgaagcagca atcgtaaccg atatcgccgg aactacgcgt gacgtgttgc
    43861 gtgagcatat ccacattgac ggaatgccgc tgcatatcat cgacaccgcc gggctacgtg
    43921 aagccagtga cgaagtggaa cgtattggga tcgagcgcgc gtggcaggaa attgaacagg
    43981 ccgaccgggt gctgtttatg gtcgatggca ccacaacaga cgccgtggat ccggcagaga
    44041 tctggccgga atttatcgcc cgtctgccag cgaaactgcc gatcaccgtg gtgcgcaata
    44101 aagccgatat caccggcgaa acgctgggaa tgagtgaagt gaacggtcac gcgttaattc
    44161 gtctctcggc aaggactggt gaaggcgtgg acgtgctgcg taaccatctc aaacagagca
    44221 tgggctttga caccaacatg gaaggcggtt tcctggcgcg ccgtcgccac ttacaggcgc
    44281 tggaacaggc agcggagcat ttgcagcaag gtaaagcgca actgttaggt gcctgggcgg
    44341 gtgaactgct ggcggaagag ttgcgcctgg cacagcagaa cttaagcgaa atcaccgggg
    44401 aatttacttc agacgacctg ctggggcgga ttttctccag cttctgtatt ggtaagtaac
    44461 cgcgcttacg aagccgtatt ctgactgtca gatgcggctt cgcttcattg ttagcactcc
    44521 tgttattcct caaccctttt tttaaacatt aaaattctta cgtaatttat aatcttaaaa
    44581 aaaagcattt aatattgctc cccgaacgat tgtgattcga ttcacattta aacaatttta
    44641 gaatagacaa aattcttgag tattatactg tagcctcgtg tctttcgagg ataagtgcat
    44701 tatgaatatc ttacatatat gtgtgacctc aaaatggttc aatatcgaca ataagattgt
    44761 cgatcaccgc ccttaattta cccttctgta gccatcacca gagccaaacc gattagattc
    44821 aatgtgaacg attagtttgc tatatcttaa ttttgccttt tgcaaaggcc atctctcgtt
    44881 tatttactta ttttagtaaa tgatggtgct tgtatatata catggcgaat taatggatat
    44941 tgcagatgta atattcacag ggatcactgt aattaaaata aatgaaggat tatgtaatgg
    45001 aaaactttaa acatctccct gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta
    45061 ccactcgcgc ttatcgtgaa gaggcaatta ttaaatccgg tatgaaccca ttcctgctgg
    45121 atagcgaaga tgtgtttatc gatttactga ccgacagcgg caccggggcg gtgacgcaga
    45181 gtatgcaggc cgcgatgatg cgcggcgacg aagcctacag cggcagccgc agctactatg
    45241 cgttagccga gtcagtgaaa aatatctttg gttatcaata caccattccg actcaccagg
    45301 gccgtggcgc agagcaaatt tatattccgg tactgattaa aaaacgcgag caggaaaaag
    45361 gcctggatcg cagcaaaatg gtggcattct ctaactattt ctttgatacc acgcagggcc
    45421 atagccagat taacggctgt accgtgcgta acgtctatat caaagaagcc ttcgatactg
    45481 gcgtgcgtta cgactttaaa ggtaactttg accttgaggg attagaacgc ggtattgaag
    45541 aagttggccc gaataacgtg ccgtatatcg ttgcaaccat caccagtaac tctgcaggtg
    45601 gtcagccggt ttcactggca aacttaaaag cgatgtacag catcgcgaag aaatacgata
    45661 ttccggtggt aatggactcc gcacgctttg ctgaaaacgc ctatttcatc aagcagcgtg
    45721 aagcagaata caaagactgg accatcgagc agatcacccg cgaaacctac aaatatgccg
    45781 atatgctggc gatgtccgcc aagaaagatg cgatggtacc gatgggcggc ttgctgtgca
    45841 tgaaagacga cagcttcttt gatgtgtaca ccgagtgcag aaccctttgc gtggtgcagg
    45901 aagggttccc gacatatggc ggcctggaag gcggcgcgat ggagcgtctg gcggtaggtc
    45961 tgtatgacgg catgagtctc gactggctgg cttatcgtat cgcgcaggta cagtatctgg
    46021 tcgatggtct ggaagagatt ggcgttgtct gccagcaggc gggcggtcac gcggcattcg
    46081 ttgatgccgg taaactgctg ccgcatatcc cggcagacca gttcccggca caggcgctgg
    46141 cgtgcgagct gtataaagtc gccggtatcc gtgcggtaga aattggctct ttcctgttag
    46201 gccgcgatcc gaaaaccggt aaacaactgc catgcccggc tgaactgctg cgtttaacca
    46261 ttccgcgcgc aacatatact caaacacata tggacttcat tattgaagcc tttaaacatg
    46321 tgaaagagaa cgcggcgaat attaaaggat taacctttac ctacgaacca aaagtattgc
    46381 gtcacttcac cgcaaaactg aaagaagttt aattaatact acagagtggc tataaggatg
    46441 ttagccactc tcttacccta catcctcaat aacaaaaata gccttcctct aaaggtggca
    46501 tcatgactga tcaagctgaa aaaaagcact ctgcattttg gggtgttatg gttatagcag
    46561 gtacagtaat tggtggaggt atgtttgctt tacctgttga tcttgccggt gcctggtttt
    46621 tctggggtgc ctttatcctt atcattgcct ggttttcaat gcttcattcc gggttattgt
    46681 tattagaagc aaatttaaat tatcctgtcg gctccagttt taacaccatc accaaagatt
    46741 taatcggtaa cacctggaac attatcagcg gtattaccgt tgccttcgtt ctctatatcc
    46801 tcacttatgc ctatatctct gctaatggtg cgatcattag tgaaacgata tcaatgaatt
    46861 tgggctatca cgctaatcca cgtattgtcg ggatctgcac agccattttc gttgccagcg
    46921 tattgtggat aagttcgtta gccgccagtc gcattacctc attgttcctc gggctgaaga
    46981 ttatctcctt tgtgatcgtg tttggttctt tcttcttcca ggtcgattac tccatcctgc
    47041 gcgatgccac cagcaccact gcgggaacgt cttacttccc gtatatcttt atggctttgc
    47101 cggtgtgtct ggcgtcattt ggtttccacg gcaatattcc cagcctgatt atttgctatg
    47161 gaaaacgcaa agataagtta atcaaaagcg tggtatttgg ttcgctgctg gcgctggtga
    47221 tttatctctt ctggctctat tgcacgatgg ggaatattcc gcgcgaaagc tttaaggcga
    47281 taatctcctc aggcggcaac gttgattcgc tggtgaaatc gttcctcggc accaaacagc
    47341 acggcattat cgagttttgc ctgctggtgt tctctaactt agctgttgcc agttcgttct
    47401 ttggtgtcac gctggggttg ttcgattatc tggcggacct gtttaagatt gataactccc
    47461 acggcgggcg tttcaaaacc gtgctgttaa ccttcctgcc acctgcgttg ttgtatctga
    47521 tcttcccgaa cggctttatt tacgggatcg gcggtgccgg gctgtgcgcc accatctggg
    47581 cggtcattat tcccgcagtg cttgcaatca aagctcgcaa gaagtttccc aatcagatgt
    47641 tcacggtctg gggcggcaat cttattccgg cgattgtcat tctctttggt ataaccgtga
    47701 ttttgtgctg gttcggcaac gtctttaacg tgttacctaa atttggctaa atccttcaag
    47761 aagccagcca ttcgctggct tcttgcctct caggaaatca cttatgtcca aatggcaact
    47821 cgcctgattc tccttcacca cgtatgcttt gcgtcacctt actatcagga cgctttagcc
    47881 catgtcccgc tttttaattt gtagttttgc cctggtttta ctttatcccg ccgggattga
    47941 tatgtacctc gttggtttac cgcgcatcgc cgccgatctc aatgccagcg aagcgcagtt
    48001 gcatattgcg ttctccgttt atctggcggg catggcagct gcgatgttat ttgccggtaa
    48061 agtggccgat cgttcaggga gaaagcctgt cgccatcccc ggcgcggcgc tatttattat
    48121 cgcttcggtg ttctgttcac tggctgaaac cagcacgtta tttcttgcag ggcgctttct
    48181 acaggggttg ggcgcaggct gttgttacgt ggtggcgttc gccattttgc gcgacacgct
    48241 ggatgatcga cgtcgggcta aagtgctgtc gttactcaac ggtattacct gcatcattcc
    48301 ggtgttagcg ccggtgctcg gacacctgat tatgcttaaa ttcccttggc agagtctgtt
    48361 ctggacgatg gcaatgatgg gcatcgcggt actgatgtta tctttgttta ttttaaaaga
    48421 aacgcgccca gcgtcccccg ctgcttcgga caaaccacga gaaaatagcg agtcgctgct
    48481 taatcggttt ttcctcagcc gtgttgttat caccaccctc agcgtttcgg tgatcctcac
    48541 tttcgtcaat acatcgccgg tattgctgat ggaaatcatg ggttttgagc gcggagaata
    48601 cgccaccatt atggcgttga ccgctggcgt cagcatgacc gtttcattct ccacgccatt
    48661 tgcgctggga atttttaagc cacgtacgtt gatgatcacc tcgcaggtgt tattccttgc
    48721 ggcggggatc acccttgccg tttcaccttc ccatgcggtt tctctgtttg gtatcacgct
    48781 gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg agtcaggcgt taggaccatt
    48841 ttcattacgc gcgggcgtag ccagctcgac cttaggtatt gcgcaggttt gcggttcgtc
    48901 actgtggatt tggctggcag cggtggttgg tatcggcgca tggaatatgc tgatcgggat
    48961 tctgattgcc tgtagcatag tgagcctgtt actgattatg ttcgtcgcgc ctggacgccc
    49021 cgttgccgct catgaagaaa tccatcacca cgcttgatct caatctgctg ctctgtctgc
    49081 aactgctgat gcaggagcgc agcgtgacca aagcggcgaa gcggatgaac gtgacccctt
    49141 cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt tgacgacccg ctctttgtga
    49201 actcaccgct ggggctgtcg cccacaccgc taatggtcag catggagcaa aatctggcgg
    49261 aatggatgca aatgagcaat ctgctgctgg ataaaccgca ccaccagaca ccgcgcggcc
    49321 tgaagtttga gttggcggcg gaatcgccgc tgatgatgat catgcttaat gcgctgtcga
    49381 aacggatcta ccaacgttac ccgcaggcga ccatcaaact acgtaactgg gattacgatt
    49441 ccttagatgc cattactcgt ggtgaagtgg atattggttt ttccggtcgc gaaagccatc
    49501 ctcgctcgcg ggagctgtta agctcgctac cgttagccat tgattatgaa gtgctgttta
    49561 gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc actgcatgaa acgtggaatc
    49621 tggacacctt tttacgttat ccgcatatca gcatttgctg ggaacagagc gacacctggg
    49681 cgctggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
    49741 aatttgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
    49801 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
    49861 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
    49921 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
    49981 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
    50041 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
    50101 atgattactc ttatattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
    50161 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
    50221 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
    50281 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
    50341 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
    50401 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
    50461 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
    50521 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
    50581 tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
    50641 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
    50701 cgttatgcga cgcggaggac gtgctagtgt ggtccgtggc ggtcacgccc gcgattgaaa
    50761 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
    50821 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
    50881 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
    50941 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
    51001 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
    51061 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
    51121 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
    51181 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
    51241 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
    51301 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
    51361 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
    51421 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
    51481 tctaaataaa aaacccgcca gcaatcatgc atggcgggtt tttaacgcgc tatcgatttt
    51541 agtgagcgtc gataaagaca atcttcagga taaacagcag cgcaacgatg attacgcacg
    51601 ggcttagatc acgcagacgt ccggtaccga ttttcatcac gcagtaggag ataaagccca
    51661 gcgcgatacc ttcggtaatc gagaagctga acggcatcat cacggcggta ataaacgccg
    51721 gaacagattc agtaagatcc tgccagttca cgcgtgccag actggaagtc atcagcacgc
    51781 caacgtaaat cagcgcacca gctgcagcgt agcctggcac catccccgcc agcggcgaca
    51841 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
    51901 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
    51961 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttac
    52021 ccttctcatc cgccaggcct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
    52081 acaggttgac cagcatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
    52141 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
    52201 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
    52261 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa gaagccgagg atacccagaa
    52321 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgggtta gcgacaatca
    52381 cacctgcgtt tttcagcccc atcatgccaa tgaacagacc gataccgctg gtaataccca
    52441 cacgcagact caccggaatg ttggcaatca tccagtagcg aacgcggaaa atcgtcagta
    52501 acagcagacc tatcgcgccc cagaagattg cgcccatccc gacctgccac ggcaagccca
    52561 tcgcctgtac aacgacaaaa gcgaagaacg cattcaggcc catagcgggt gccagtgcaa
    52621 ctggcaggtt agcaaacagt cccatcataa tactgccgaa tgcagcgatc agacaggtag
    52681 tgacgaagac ggcgctggta tccatgccag caacgccaag aatttgcggg ttaacaaaaa
    52741 cgatgtaaac catcgtcagg aaggtggtaa aaccggcgat cacttcggtc cgtgccgtcg
    52801 tgccatgttc gcgcagttta aacacgcgtt ccagcatccc ctggccagaa gcctgggttg
    52861 tgtgttgttg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
    52921 ccaaaatgag acaatatgga cggctgaaga taactttttt tcattgtatt tgcttatacg
    52981 gcaacgattg cgtcatgcga attggcatga cgaaaacgtt aaactaacga taaaggaaag
    53041 acatgtccca gatagaagcg gtatttttcg actgcgacgg tacgctggtc gacagtgaag
    53101 tcatttgctc ccgcgcatat gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg
    53161 aagaggtatt caaacgtttc aaaggtgtaa aactgtacga aattatcgat attgtttccc
    53221 ttgaacatgg tgttacgtta gcgaaaacag aagctgaaca cgtttaccgt gcagaagtcg
    53281 ctcggctgtt cgattcagaa ctggaagcca tcgaaggggc tggagcgctc ctgtcagcga
    53341 tcactgcgcc aatgtgtgtg gtatctaacg gcccaaataa caaaatgcag cattctatgg
    53401 gcaagctgaa tatgttgcac tacttcccgg ataaactgtt cagcggctac gatattcagc
    53461 gctggaagcc agacccggcg ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag
    53521 aaaactgcat tctggttgat gactcagttg ccggtgcgca atctggtatc gacgcaggta
    53581 tggaagtgtt ctacttctgc gccgacccgc acaataagcc gatcgttcac ccgaaagtca
    53641 ccacctttac ccatctttcg cagttaccgg aactgtggaa agcgcgtggt tgggatatta
    53701 cggcatagtt cttcacactc ccttcactta ccccgcttaa attggcgctc aaaggtaagt
    53761 aaagggagtt tgatatgtct gtttcacgtc gggtaataca tcacggactt tattttgcgg
    53821 ttttaggacc gttaattggt gttctgtttc ttgtcctcta catattcttc gcaaaagaac
    53881 cgctggttct tttgcgaaga atacaggtac ttccattgtt tctcttattg tcgattacta
    53941 cgggagttat tcctgcattg ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg
    54001 ggtcacagaa aaattatcgt tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga
    54061 tctattgtgc agttattgta catattaagg gcatggcttc ctcggagttg tatgaaaaca
    54121 ttctttctgg tgacagtctc gttgtccgca tcattcctgc attgctggca ggtgtggtga
    54181 tgagcagaat cattacccgt ctgcccggat tggatatttc atgtcctgaa acagactctt
    54241 taagttaagc gggatacttt atctttgggc tactcaaaag cagacaggat gttactatga
    54301 ctcaaaatat caggccgtta ccccaattca aatatcatcc caagccactg gaaacaggcg
    54361 catttgaaca ggataaaacc gtagagtgcg attgctgtga acaacagacg tcagtttatt
    54421 actcgggtcc cttttattgc gttgatgaag ttgaacatct ctgtccgtgg tgtattgcgg
    54481 acggttctgc tgctgaaaag tttgcaggta gttttcagga tgatgccagc atagaaggtg
    54541 ttgaatttga gtatgatgaa gaggacgaat ttgccggtat taagaacaca tatcctgatg
    54601 aaatgctgaa agagttggtt gaacgcacgc caggttatca tggatggcaa caggaattct
    54661 ggcttgcgca ttgtggcgat ttctgtgctt ttatcggcta tgtgggctgg aatgatataa
    54721 aagatcgcct cgatgaattt gccaaccttg aagaagattg tgagaatttc ggtattagaa
    54781 attctgatct agctaaatgc ctgcaaaagg gtggtgattg tcagggttat ctcttccgct
    54841 gtctccactg cggcaagctg agactgtggg gtgatttttc gtagttattt aaataatgag
    54901 aacaggccgg agcgtaattc atacatccgg ccttatttct tcagcttaat cgagcgctaa
    54961 ttctgcggca gcggctttat cagcgattac catcagcgaa ggatgcagct gcaacacgga
    55021 agctggaaca tcttcggtaa ccggaccttg cagcacattt ttcagtgcct gcgctttacc
    55081 tgcgccactg acaattatca gcaagttttt cgccgccatg atgcttttcg gccccatcgt
    55141 cacgtagctg tcaggcacta acgaaaagtc gccgcccaac tcaccatgcg ccacaatatc
    55201 aaccatctct ccctggatcg ggaactccac ggtttgctca tggaaatggg tcgtattcgg
    55261 caaattgcca caaaaatgac catctgcacc taatcccagc accaccaaat ccaggccgcc
    55321 ttcacgcgcc agtttctgat catgctcgcg gtagttatca atagtgagct tctggatatt
    55381 ctcttctttg atccccgcag gggtgaaaaa cagattacgc agattggtaa tcgttacacc
    55441 ttctccctct ttgccgcgaa agggaatttc atcgaaatta tagaaatagc agttatcata
    55501 ccagggctta cctttaacca gggtggtgag gtattcatac atgcctttgg gcgtgctacc
    55561 ggcggtaatt gccaggttaa cacgacgcgt cttcgacata taaccaagca gatggtgtgc
    55621 cgcgacacgg ctcatttcct ggtaatcttc ggtaatgatt aatttcatct tagtgcctta
    55681 ttttatttaa ataatttctg tacgaaggct gcataggcag gccgccagac atccatttca
    55741 tggttcaggc cgggatattc ctggtaatca aacttaattt ttttctgctc aagctcagtt
    55801 ttcagcccgg cgatatcctt gccggttacg acatctttat caccaacgac cacagtaaaa
    55861 ttacgtagtt gctggttaat agctgccgga tcgttcagcc gggccgcgac accttcatcc
    55921 ggtacggttg tcgtggtaac accactgaat gtggccagcc agccaaagct ttccagatga
    55981 ttcattccgg aaaccagcgc ctggtacccg ccttgtgaaa gccctgccag cgcgcgacca
    56041 tcggcatctt tacggacatt aaaacgcttg ctaatcagcg ggataatatc gttcatcagt
    56101 tcgcgatctg ccgctttagc attcagcgga taaaagactt tacgtctttc ctgaggcacg
    56161 aaatcttcgg gaataatgcc cttcgcatcg gtttcggtat ccgggatcac caccagcatc
    56221 ggtttaattt tcccttcagc cagcaggtta tccatgattt gcgggatacg cccctgatcg
    56281 atagcggaac gtccggtatc accaaaaccg tgatagaaat agagcactgg caaaggctcg
    56341 cccatgccgg tgtaacctgg cggggtccag acatacatct gacgttcaga ttgcaatgcg
    56401 ttggagtggt aagttatggc gatcaaatca ccatgcgcaa cagaacgcgt atccagataa
    56461 ctgcctggca ccagaatcat actggagtta acctggcgct gaggcttagt cattgcggtg
    56521 cctgtatcaa tgctgcgtac accatcaaca ttgaaaaaat actcgtacag gttgcctttc
    56581 aggataggtg tgcgccacga ccagactcct gcttcgtctt tggtcatcgg gtgaatattg
    56641 tccggaaccg gaacacccac cactacagag acattttttg ccccaggggc aaagtagcga
    56701 aaggtcacgc tgttatcggc attgacctga gtcacatact gtttcaccgg gatagtgggt
    56761 gccgggctgg ctggcatatc cgctgctatg gcccactgag cagaaatacc gctggcaata
    56821 gccagcgtta aagcagcaat ttttatattc atcgtaatat tcctttcatt aagttccatt
    56881 accaccagat ttcagcctgg gcaccgacag cgaactggtc gtctttattg tcttcgaagg
    56941 tgaagccatc cagttcgttt tccagggctt tgatatacgt ggcgtagaaa cgaatttccg
    57001 gacgcgaggt caacatactg gtattgactt taaaggtatg gaagagcgtg gttttataac
    57061 cagactcatt aaatttatta ctattcgcat ctttgttttg ctgggtgaaa tagcccagtt
    57121 caacacctgt ctggttatat tggtcccaaa tataggccgg gcgaacaacc gcacgaatag
    57181 attcgaaatc agagtgtgcg cctgtttcgt agctataaat atcgttaccg aaggagtaaa
    57241 caatcgcgtt agccacaata aaatgatcgc cgatataggc ttcgccctgt gaagtcagac
    57301 gaactgctgt tccgccggtg tgatcaccat aataacgacc attaaaggtg gtaaatggac
    57361 ttgcgccagc ataacggcca aagttactgg cgatagagtt attcgcaacc aggaaggaga
    57421 attcgttgaa gccaccttta tcaaatttct gcgttaaaga tgtgccaaac atccaggtat
    57481 ctttccagtc ataatagccg ttattatcct gattatcttt ttccgatgcg ctttcgtttg
    57541 ccgtgacata acgaccactc accattaagg tcgctttatc ccataacggg atatctttat
    57601 agcgtaaatc aatagtattg gtattaatct gctgtttgtt ttgcaggctg cgatcgtaat
    57661 catcgacatc ttcgcgaacc agcgcgatat caattttacc cggaccgact ttccagtttt
    57721 ccagacctac acccgctgcg gcatcagtac gctgcgtttt ccagtccagc atctggattt
    57781 caattttcgg cgcaccgtgt ttacccaccc agaaatcagc ctctggcgca aagggcagga
    57841 aacctttggt ggtaacgtac atatcggaga actgcataaa gttctcgcca ccggcgttat
    57901 cgccaaacca gccggtagag tactgctgac caacgttacc atccatcatc acaacggcat
    57961 caacccgttt gccgttttcg ttgtagacac gttgttttaa ctgcaaatca aaccagccgg
    58021 agtattcgtt accaaagcgg cccagagaac caatcgccca tgatttaggt gaaccatgag
    58081 aggcggtccc ccaaccagaa cggtaatagc cgttgtaact aaagccaatt tcatctttca
    58141 caaacttact gaaatctttc agagtcatgg aagaataaat cgagcctgtc tcactggcat
    58201 ttttctcttc atttttgacc agtacagcat caggttttgc ggcactactg gtcgggaacg
    58261 gattggcggc atacccttga tcattcgtac ttacgctacg attcaccgtc gctggcgtat
    58321 attttttctt ctcttcatct ttatactttt tgagttcgct ttgcgtttct cttaacgctt
    58381 tttctaataa ctcaaggcga tgttccaccg ttaatgattc agccaatgat tgtgcagaaa
    58441 aggctaacgg tgccattaat aagatggcag aggtaataag atttcgtcta aacataatat
    58501 ccctttatgg tgcaaagaaa gaattaacgt atcgcatcca gactgttctg aatgcgacga
    58561 taattaaggt gctttattgg ttattttttt aatgacagcc cccgcgtttt gatcacttct
    58621 gcataccatc cgaagctttt cttacgcgtt cttgtgaggc ttccttcgcc attatcatca
    58681 cgatccacat aaataaagcc gtagcgctta gacatttgtg aatgagatgc actgactaaa
    58741 tcaattggcc cccaactggt gtaccccata atatccacac catcggcaat cgcttcattt
    58801 acctgtacca ggtgatcgtt taaataggca attcgataat cgtcctgtat cgaaccatcc
    58861 gcttcaacgc tgtcttttgc gcctaatccg ttctcgacaa taaataacgg tttttgataa
    58921 cgatcccaaa gcgtatttaa cagaacccgt aatccaaccg aatcaatttg ccacccccac
    58981 tctgaacttt tcagatgcgg attggggatc atattcagta tgttgccctg cgcattttta
    59041 ttaatgcttt cgtcgtggga aacacaacca gtcatgtaat aactaaaaga gatgaaatcg
    59101 acggtatgtt ttaaatcttc tgcgtcactt tcagtcatct caatggtgat attgtggtcg
    59161 cggaagaaac gctgcatata gccgggatac tggccacgcg cctgaacatc accaaagaac
    59221 atccagcgcc ggttctcttc catggcctgc aacatatcct gtggctggca ggtgaggggg
    59281 taaaccagcc caccgagaag catattgccg attttcgctt cggggagcag gctatgacag
    59341 gctttaactg cccgcgcact ggcaaccagt tgatggtgga tagcctgata aacttccgcc
    59401 tcgccactct cttctgccag ccccacgccc gtgaatggct cgtgtaacga catgttgatt
    59461 tcattaaacg tcagccataa cgccacttta tgttggtagc gagtaaagac cgtgcgggcg
    59521 taatgctcga agtgatcgat gaccgctcga ttagcccaac cgccgtagtt tttcaccagc
    59581 ccatatggca tttcgtaatg ggataacgtt accagcggct tgatccccgc ctgcgccatt
    59641 tcatcaaaca gccgatcgta aaacgctaac cccgcttcat tcggttcgac ttcgtcgccc
    59701 tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac aggtgaagcc catctcggca
    59761 aataacgcga tatcttccgg gtaacggtga taaaaatcga tggcgacatc tttgatattc
    59821 tctttcccca ggatgcgcgg ttccattttt cccattacgc catgaggctg taaatctgag
    59881 gtcgagatcc ctttgccatc ttcctgccag gcaccttcca cctgattggc agctgttgcg
    59941 ccaccccaaa gaaatgtttc tggaaatgct ttcataatta actcctttta tcgttagcga
    60001 atgatggata acagcggttc acctgcgctt atctgcgccg tgccgtgggg taatacgtcc
    60061 gtaaaatcat cgctattact gattaatacc ggcgtcgtca gatcaaatcc ggcctcgcga
    60121 atagcaggga tatcaaaaga aatcagccga tcgcctgtat tgaccttgtc acccacgttg
    60181 acgtgagcgg aaaagaattt gccgtccagt tttacggtgt cgataccgac atgaatcagg
    60241 agctccacac catcatctga ctcaatgcca atggcgtgta atgtggcgaa caacgaagca
    60301 attcgacccg caaccggaga acgcacttca ccaaccgagg gcagaatggc gataccttta
    60361 cccaacaggc cactggcaaa cgtggtatca gcgacgtgaa tgagcggcac aatctctccc
    60421 gtcatcggtg aacagatacc gccctgctca ggtggtgtaa taacctctgg tgttttctct
    60481 tgcggggcac cctgcgctgg ctgacgttta gcggtgatga aatgaagcat caccgtaccg
    60541 acaaatgcgc aaccgatggc aatgacaccg ccaataacgc tggcccagac ggtgaaatca
    60601 attcccgttg acgggatggt ttgcatgaag gtgaaaatac ttggcaaacc aaaggagtac
    60661 actttcgttt gcgcgtagcc aatgatggtg gcccccaaag ccccactgat acaggcgata
    60721 acaaaggggt acttacgcgg cagattaacg ccatataccg ctggttcggt gataccaaac
    60781 aggcccgtca acgccgctga tcccgccacc acttttttct gcgcatcgcg ttcgcagagg
    60841 aagacgccga gcgccgcccc gacctgcgcc ataatggcgg gcattaacag cgggatcatg
    60901 gtgtcgtagc ccagcacggt gaagttattg atacacagcg gcaccaggcc ccagtgcagt
    60961 ccgaacatga cgaagatttg ccagaagccg cccattaccg cgcctgcaaa tgcaggaacc
    61021 gcctgataaa gccagagata accggcggca atcagttcgc ttatccaggt tgatagcggc
    61081 cccaccagca gaaaggtgac gggtgtgata accatcagac atagcaatgg tgtgaagaaa
    61141 tttttgattg ccgacggtaa ccacgcatta agtcggcgtt ccagaatgct gcacaaccag
    61201 gcagaaaaaa taatgggaat aaccgatgac gagtaattca acaatgtgac cggaataccc
    61261 aggaaatcca gccccagctc atccgctttt tgcccgttct cgaaagcagt cagaattaat
    61321 ggatgcacta acgctccacc aatcaccatg gcagtaaatg gattaccgcc gaagcgtttc
    61381 cccgcggtgt atcccaggat tatcgggaag aaccaaaaca aggcatcact ggcgctgaat
    61441 aaaattaaat aagtaccact ttgttcggtc gtccactgaa aagtgagcgc cagagccagc
    61501 atacctttca ggatcccggt tgccgccatc aaaccgatca gaggcgtaaa aatacctgaa
    61561 ataacataaa caaagcggtt tagcagatta cctttctcat cattttccgg tgcctgttgc
    61621 gctttttcgc caaggcctgc cacgctgttg accgccagga agacatcggc cacatggtta
    61681 cctatgacca cctgaaactg accaccgctt tccaccacca taataatacc gggggtcttt
    61741 ttcagtacct cagcttgcgc tttgctttca tcctttaact taaaacgtaa tcgcgttgcg
    61801 caatgcatca gactcacaat gttatctgcg cccccgactc ctgcgactat ttttctggct
    61861 aactccgtca taacttgccc tctaccgctt tgcggcaaaa ctccaaaaaa aaacctgaaa
    61921 aaaacggcct gacgtgaatc aagcaatttt tttcaggttt tgcccgctta gtgcggtaac
    61981 aatcctttac tcagtaataa tatttcagtg ttctttgcgc acgcgctcta tatttatggc
    62041 taaaaacata atctctgcgg gtgaaatttt acgttgatac tgcaaaccaa taaaaatggc
    62101 gatccgttcc gcacattgcc atgcttgcgg gtaattttgt tttactgctt gttgtaatga
    62161 ttcatcacta tcgttaattg aagcatgttc aagaatacgc caggataaaa acttcagatg
    62221 tgtaaccagt cgctgataac tcaagctttc ttcctggtaa ttaaggctga actgaaattt
    62281 tattaattgc agcatttcgc gcattaactg cgtgacacct gcaacatcct ccatatttcc
    62341 gctcatttgg gcactgacca gatgcatggc aataaagccc acttcatctt tcggtaactg
    62401 cacgcccaac cgtttatcaa taatggttaa cgcttcttcc cctagctgga actctttcgg
    62461 gtaaagccgc tggatatccc acagcaacgg gttgggcagc aacacgtttt gctgaaagcg
    62521 tttaatcgca aactggcaat ggtcagttag cgagatataa atactgtcct gtaattttcc
    62581 caagcgctcc tgcgctaaag agataatacg atcacaggtt gccatcacct caagaggaat
    62641 atgacttaag agttcgctta atcgcccgtt cagttcatga ctgctcaagg catactcttt
    62701 ttctattcca cttgagttaa ttctttcgcc agcgcgtttt tgaaagccaa ttcctcgccc
    62761 catgacgact ttttcccgct gttgatcatc aataaccacc acaacattat tgttgagaat
    62821 tttggtgatt tgcatgttca tagcaaggac cttttttata aacaaaaaaa cccgacttca
    62881 ccagtattct ctggttatgt caggttttgc ctgcgaatgc agtaacaatc cagtcattta
    62941 ttaatggttt ttataacgaa catccaggtt cggaaattaa tttaattgcg tgcagagaaa
    63001 atagcaatgc gctattgata aaaata
//
LOCUS       DAZFKM010000011        58666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFKM010000011 DAZFKM010000000
VERSION     DAZFKM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..169
                     /gene="yhfL"
                     /locus_tag="ACHKAY_000802"
     CDS             2..169
                     /gene="yhfL"
                     /locus_tag="ACHKAY_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO5699519.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            464..1801
                     /gene="frlA"
                     /locus_tag="ACHKAY_000803"
     CDS             464..1801
                     /gene="frlA"
                     /locus_tag="ACHKAY_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO5699520.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1822..2844
                     /gene="frlB"
                     /locus_tag="ACHKAY_000804"
     CDS             1822..2844
                     /gene="frlB"
                     /locus_tag="ACHKAY_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO5699521.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2894..3724
                     /gene="frlC"
                     /locus_tag="ACHKAY_000805"
     CDS             2894..3724
                     /gene="frlC"
                     /locus_tag="ACHKAY_000805"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO5699522.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3721..4506
                     /gene="frlD"
                     /locus_tag="ACHKAY_000806"
     CDS             3721..4506
                     /gene="frlD"
                     /locus_tag="ACHKAY_000806"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO5699523.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4606..5337
                     /gene="frlR"
                     /locus_tag="ACHKAY_000807"
                     /gene_synonym="yhfR"
     CDS             4606..5337
                     /gene="frlR"
                     /locus_tag="ACHKAY_000807"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO5699524.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5489..6574)
                     /gene="yhfS"
                     /locus_tag="ACHKAY_000808"
     CDS             complement(5489..6574)
                     /gene="yhfS"
                     /locus_tag="ACHKAY_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5699525.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6586..7890)
                     /gene="yhfT"
                     /locus_tag="ACHKAY_000809"
     CDS             complement(6586..7890)
                     /gene="yhfT"
                     /locus_tag="ACHKAY_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO5699526.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7902..8255)
                     /gene="yhfU"
                     /locus_tag="ACHKAY_000810"
     CDS             complement(7902..8255)
                     /gene="yhfU"
                     /locus_tag="ACHKAY_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO5699527.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8266..9144)
                     /gene="php"
                     /locus_tag="ACHKAY_000811"
     CDS             complement(8266..9144)
                     /gene="php"
                     /locus_tag="ACHKAY_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO5699528.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9141..10367)
                     /gene="yhfW"
                     /locus_tag="ACHKAY_000812"
     CDS             complement(9141..10367)
                     /gene="yhfW"
                     /locus_tag="ACHKAY_000812"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5699529.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10367..11530)
                     /gene="yhfX"
                     /locus_tag="ACHKAY_000813"
     CDS             complement(10367..11530)
                     /gene="yhfX"
                     /locus_tag="ACHKAY_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO5699530.1"
                     /translation="MFVEALKRQNPALISAVLSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11614..11976)
                     /gene="yhfY"
                     /locus_tag="ACHKAY_000814"
     CDS             complement(11614..11976)
                     /gene="yhfY"
                     /locus_tag="ACHKAY_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5699531.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11993..12898)
                     /gene="yhfZ"
                     /locus_tag="ACHKAY_000815"
     CDS             complement(11993..12898)
                     /gene="yhfZ"
                     /locus_tag="ACHKAY_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO5699532.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13167..14171)
                     /gene="trpS"
                     /locus_tag="ACHKAY_000816"
     CDS             complement(13167..14171)
                     /gene="trpS"
                     /locus_tag="ACHKAY_000816"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO5699533.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14164..14922)
                     /gene="gph"
                     /locus_tag="ACHKAY_000817"
     CDS             complement(14164..14922)
                     /gene="gph"
                     /locus_tag="ACHKAY_000817"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO5699534.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14915..15592)
                     /gene="rpe"
                     /locus_tag="ACHKAY_000818"
     CDS             complement(14915..15592)
                     /gene="rpe"
                     /locus_tag="ACHKAY_000818"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO5699535.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15610..16446)
                     /gene="dam"
                     /locus_tag="ACHKAY_000819"
     CDS             complement(15610..16446)
                     /gene="dam"
                     /locus_tag="ACHKAY_000819"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5699536.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16553..17839)
                     /gene="damX"
                     /locus_tag="ACHKAY_000820"
     CDS             complement(16553..17839)
                     /gene="damX"
                     /locus_tag="ACHKAY_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO5699537.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17931..19019)
                     /gene="aroB"
                     /locus_tag="ACHKAY_000821"
     CDS             complement(17931..19019)
                     /gene="aroB"
                     /locus_tag="ACHKAY_000821"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO5699538.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(19076..19597)
                     /gene="aroK"
                     /locus_tag="ACHKAY_000822"
     CDS             complement(19076..19597)
                     /gene="aroK"
                     /locus_tag="ACHKAY_000822"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO5699539.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19998..21236)
                     /gene="hofQ"
                     /locus_tag="ACHKAY_000823"
     CDS             complement(19998..21236)
                     /gene="hofQ"
                     /locus_tag="ACHKAY_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO5699540.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21148..21552)
                     /gene="hofP"
                     /locus_tag="ACHKAY_000824"
     CDS             complement(21148..21552)
                     /gene="hofP"
                     /locus_tag="ACHKAY_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO5699541.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21542..21982)
                     /gene="hofO"
                     /locus_tag="ACHKAY_000825"
     CDS             complement(21542..21982)
                     /gene="hofO"
                     /locus_tag="ACHKAY_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO5699542.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21966..22505)
                     /gene="hofN"
                     /locus_tag="ACHKAY_000826"
     CDS             complement(21966..22505)
                     /gene="hofN"
                     /locus_tag="ACHKAY_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO5699543.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22505..23284)
                     /gene="hofM"
                     /locus_tag="ACHKAY_000827"
     CDS             complement(22505..23284)
                     /gene="hofM"
                     /locus_tag="ACHKAY_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO5699544.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23404..25956
                     /gene="mrcA"
                     /locus_tag="ACHKAY_000828"
     CDS             23404..25956
                     /gene="mrcA"
                     /locus_tag="ACHKAY_000828"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO5699545.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26122..26682)
                     /gene="nudE"
                     /locus_tag="ACHKAY_000829"
     CDS             complement(26122..26682)
                     /gene="nudE"
                     /locus_tag="ACHKAY_000829"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO5699546.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27002..29137
                     /gene="igaA"
                     /locus_tag="ACHKAY_000830"
     CDS             27002..29137
                     /gene="igaA"
                     /locus_tag="ACHKAY_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO5699547.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29202..29870
                     /gene="yrfG"
                     /locus_tag="ACHKAY_000831"
     CDS             29202..29870
                     /gene="yrfG"
                     /locus_tag="ACHKAY_000831"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO5699548.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29881..30282
                     /gene="hslR"
                     /locus_tag="ACHKAY_000832"
     CDS             29881..30282
                     /gene="hslR"
                     /locus_tag="ACHKAY_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO5699549.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30307..31185
                     /gene="hslO"
                     /locus_tag="ACHKAY_000833"
     CDS             30307..31185
                     /gene="hslO"
                     /locus_tag="ACHKAY_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO5699550.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31320..33044)
                     /gene="yhgE"
                     /locus_tag="ACHKAY_000834"
     CDS             complement(31320..33044)
                     /gene="yhgE"
                     /locus_tag="ACHKAY_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO5699551.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33423..35045
                     /gene="pckA"
                     /locus_tag="ACHKAY_000835"
     CDS             33423..35045
                     /gene="pckA"
                     /locus_tag="ACHKAY_000835"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO5699552.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35121..36473)
                     /gene="envZ"
                     /locus_tag="ACHKAY_000836"
     CDS             complement(35121..36473)
                     /gene="envZ"
                     /locus_tag="ACHKAY_000836"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO5699553.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36470..37189)
                     /gene="ompR"
                     /locus_tag="ACHKAY_000837"
     CDS             complement(36470..37189)
                     /gene="ompR"
                     /locus_tag="ACHKAY_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO5699554.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37417..37893
                     /gene="greB"
                     /locus_tag="ACHKAY_000838"
     CDS             37417..37893
                     /gene="greB"
                     /locus_tag="ACHKAY_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO5699555.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37990..40311
                     /gene="yhgF"
                     /locus_tag="ACHKAY_000839"
     CDS             37990..40311
                     /gene="yhgF"
                     /locus_tag="ACHKAY_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO5699556.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40749..40976
                     /gene="feoA"
                     /locus_tag="ACHKAY_000840"
     CDS             40749..40976
                     /gene="feoA"
                     /locus_tag="ACHKAY_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO5699557.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40993..43314
                     /gene="feoB"
                     /locus_tag="ACHKAY_000841"
     CDS             40993..43314
                     /gene="feoB"
                     /locus_tag="ACHKAY_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO5699558.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43314..43550
                     /gene="feoC"
                     /locus_tag="ACHKAY_000842"
     CDS             43314..43550
                     /gene="feoC"
                     /locus_tag="ACHKAY_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO5699559.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43753..44631
                     /gene="rpnA"
                     /locus_tag="ACHKAY_000843"
     CDS             43753..44631
                     /gene="rpnA"
                     /locus_tag="ACHKAY_000843"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO5699560.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44660..45430)
                     /gene="bioH"
                     /locus_tag="ACHKAY_000844"
     CDS             complement(44660..45430)
                     /gene="bioH"
                     /locus_tag="ACHKAY_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO5699561.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45468..46151
                     /gene="gntX"
                     /locus_tag="ACHKAY_000845"
     CDS             45468..46151
                     /gene="gntX"
                     /locus_tag="ACHKAY_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO5699562.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46210..46785
                     /gene="nfuA"
                     /locus_tag="ACHKAY_000846"
     CDS             46210..46785
                     /gene="nfuA"
                     /locus_tag="ACHKAY_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO5699563.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47146..48462
                     /gene="gntT"
                     /locus_tag="ACHKAY_000847"
     CDS             47146..48462
                     /gene="gntT"
                     /locus_tag="ACHKAY_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5699564.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKILADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48507..50591)
                     /gene="malQ"
                     /locus_tag="ACHKAY_000848"
     CDS             complement(48507..50591)
                     /gene="malQ"
                     /locus_tag="ACHKAY_000848"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO5699565.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50601..52994)
                     /gene="malP"
                     /locus_tag="ACHKAY_000849"
     CDS             complement(50601..52994)
                     /gene="malP"
                     /locus_tag="ACHKAY_000849"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO5699566.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53606..56311
                     /gene="malT"
                     /locus_tag="ACHKAY_000850"
     CDS             53606..56311
                     /gene="malT"
                     /locus_tag="ACHKAY_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO5699567.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56354..57370)
                     /gene="rtcA"
                     /locus_tag="ACHKAY_000851"
     CDS             complement(56354..57370)
                     /gene="rtcA"
                     /locus_tag="ACHKAY_000851"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO5699568.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57374..58600)
                     /gene="rtcB"
                     /locus_tag="ACHKAY_000852"
     CDS             complement(57374..58600)
                     /gene="rtcB"
                     /locus_tag="ACHKAY_000852"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO5699569.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        13900 a        16174 c        14816 g        13776 t
ORIGIN      
        1 aatgaacaaa tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg
       61 tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc
      121 aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag
      181 aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg
      241 tttctatgtc cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata
      301 actacatcca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta
      361 cataacatca tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc
      421 aaactctccc ctacaacgta ttggacaaga aaaaggtatc gctatgggaa gccaggaact
      481 ccaacgcaag ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa ccgtcggctc
      541 cggtattttt gtatctgtgg gtgaagtggc aaaagcagcg ggcacgccgt ggcttacggt
      601 gctcgcgttt gtcattggcg ggttaattgt gatcccgcaa atgtgcgtct atgcggaact
      661 atccaccgct tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg ccggaagccg
      721 accgctggct ttcctctccg gctgggccag cttctgggcc aacgatgcgc cgtcattgtc
      781 gattatggcg ctggcgattg tcagcaatct tggcttttta acgcctatcg atccgttgct
      841 cggtaaattt atcgccgccg gattaattat cgcctttatg ttgctacacc tgcgctccgt
      901 tgaaggcggc gcagcgtttc agacgctaat taccatcgcc aaaattatcc cgttcactat
      961 cgtcattggc cttgggatct tctggtttaa agcggagaat tttgccgccc ctaccaccac
     1021 tgcgattggc gcaacgggca gctttatggc gctgctggcg gggatctctg ccaccagttg
     1081 gtcgtatacc ggcatggcct ctatctgtta tatgaccggc gaaattaaaa accccggaaa
     1141 aaccatgcca cgagcgctga ttggttcctg tctgctggtt ctggtgctct acaccctgct
     1201 ggcgctggtg atttccggcc tgatgccctt cgacaaactc gccaattctg aaacgccgat
     1261 ttccgacgcc ctgacctgga tccccgcact cggcagcacc gctgggatct ttgttgccat
     1321 cacggcgatg atcgtcattc ttggttcgct ttccagctgc gtgatgtacc agccgcggct
     1381 ggaatacgcg atggcgaaag acaacctgtt ctttaaatgc ttcggccatg tgcatccgaa
     1441 atacaacacg ccggatgtct ccatcatcct gcaaggggcg ctggggatct tcttcatctt
     1501 cgtttccgat ctcaccagcc tgctgggtta tttcaccctg gtgatgtgtt tcaaaaatac
     1561 cctcaccttc ggctccatca tctggtgtcg taaacgcgac gattacaaac cgctgtggcg
     1621 tactccggct ttcgggctga tgaccaccct cgccattgcg tcaagcctca ttctggtcgc
     1681 ctcaaccttt gtctgggcac cgattcccgg ccttatctgc gccgtcatcg ttattgctac
     1741 tggtctgcct gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg ctttgtcgta
     1801 atgttatctg gagaaaataa aatgttggat attgataaaa gcaccgtgga ctttctggtc
     1861 accgaaaata tggttcagga agtggaaaaa gttctcagcc atgacgttcc gctggtgcac
     1921 accatcgtgg aagagatggt gaagcgcgac attgatcgta tttatttcgt tgcctgcgga
     1981 tcgccactca acgcggcgca aacggcgaaa catctggcgg atcgcttttc cgatcttcag
     2041 gtctacgcca tttccggctg ggagttctgc gataacaccc cgtatcgcct cgacgatcgt
     2101 tgcgcggtga ttggcgtttc tgactacggt aaaacggaag aggtaatcaa agcgctggag
     2161 ctgggccggg cctgcggcgc actcactgcg gcgttcacca aacgcgcgga cagcccgatt
     2221 acctcggcgg cggaatttag cattgattat caggccgact gtatctggga aattcacctg
     2281 ctgctctgct acagcgtggt gctggagatg atcacccgcc tcgcgccgaa cgcggaaatc
     2341 ggcaagatca aaaacgatct caagcagttg ccgaatgcgc tcggtcaact ggtacgcacc
     2401 tgggaagaaa aaggccgcca gcttggtgaa ctggccagcc agtggccgat gatttatacc
     2461 gttgctgcgg gtccgctgcg tccgctgggt tacaaagaag gcattgtaac gctgatggaa
     2521 tttacctgga cgcacggctg cgtgattgag agcggagagt tccgccatgg cccgctggag
     2581 attgtcgaac cgggcgttcc gttcctgttc ctgctcggca atgatgaaag tcgccacacc
     2641 accgaacgcg ccattaactt tgttaaacag cgtactgaca acgtgatcgt catcgattac
     2701 gccgaaattt cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt cgtgccaatg
     2761 gagtggctct gctactacct gtctatttac aaagatcaca acccggatga acgccgctat
     2821 tacggtggtc tggtggaata ttaatccctc tcccggcccg gcaacgggcc gaatttacgc
     2881 aaggagttac ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga
     2941 acacgcattt cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt ggggcggtcg
     3001 cccgcacgcg ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc
     3061 gcagacgtat cagatgccga ttatcggcta tacgccagaa accaacggct atccgtataa
     3121 catgatgctg ggcgatgaac atatgcgtcg cgaaagcctc gacatgatca agctggcgat
     3181 ggatatggca aaagagatga acgcgggtta tacgctgatt tccgcggccc acgcgggcta
     3241 tctcacgcca cctaatgtta tctggggacg gctggcagag aacctaagcg aactgtgtga
     3301 gtacgcggaa aacatcggca tggatctaat cctcgaaccg ttaacgccgt atgaatcgaa
     3361 cgtcgtgtgt aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct
     3421 gttcagcatg gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt
     3481 cgacaaactg ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga
     3541 cacgcattac attcctggag aaggcaaaat gccgctgagg gaactgatgc gcgatattat
     3601 cgaccggggc tatgagggtt actgtacggt ggagctggtg acgatgtata tgaacgagcc
     3661 cagactctat gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagag
     3721 atgaaaaccc tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa
     3781 gcgttttctg gcggtaatgc ggtcaatgtg gcggtgtact gcactcgcta cggcatacag
     3841 ccgggatgca ttacctgggt gggtgacgat gactacggca caaagctgaa gcaggatctc
     3901 gcccgcatgg gcgtcgatat cagccatgtc catacgaaac acggcgttac cgcacaaact
     3961 caggtggaac tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg cgtgatggcc
     4021 gactttgccc tgagtgaaga ggattacgcc tggctggcgc agtatgacat tgtgcacgcg
     4081 gcaatctggg gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc
     4141 gctttcgact tctccgacaa gtgggacagc tcgctctggc agacactggt gccgcatctc
     4201 gattttgcct ttgcctccgc accgcaagaa gacgaagcgc tgcgtctgaa gatgaaagcg
     4261 attgttgccc gtggcgcagg cgtggtgatt gtcacgctgg gtgaaaacgg cagcattgcc
     4321 tgggatggcg cgcagttctg gcgtcaggct cctgaaccgg tgacggttat cgacaccatg
     4381 ggtgccggag attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta
     4441 ccgcaggcga tggcgcaggg aacggcgtgc gcggcgaaaa ccattcagta ccacggtgcc
     4501 tggtaggtat aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa
     4561 cgaccctata atccgagtaa ttcattcttt atttcagggt cgattatgtc agctacggac
     4621 cgctactctc atcaactcct ctacgctacc gtccgccagc ggttgctgga tgatatcgcg
     4681 cagggggttt accaggccgg gcaacagatc cctaccgaaa acgagctttg tacgcaatat
     4741 aacgtcagcc gcattactat tcgcaaagct atcagcgact tagtggcaga cggcgtactg
     4801 atccgctggc agggcaaagg cacctttgta caaagccaga aggtcgaaaa cgccctgctg
     4861 accgtcagtg gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg
     4921 atcgagcagg aacgagtcag cgccgcgccg ttttgcgaaa agctgaacat ccctggcaac
     4981 agcgaagtgt tccatctttg ccgggtgatg tatctcgata aagagccgct gtttattgat
     5041 agttcctgga tcccactgtc gcgttatcct gactttgatg agatttacgt tgaaggaagc
     5101 tccacttatc agttatttca ggagcgtttc gacacgcgag tggtcagcga caaaaagacc
     5161 atcgatatct ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga actgggcgaa
     5221 ccgttgtttc gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc
     5281 gaactcttct gccgcgccaa tcgcatcacc ttaacgattg ataataaaag acattaaccg
     5341 taggccggat aagatgcgcc agcatcgcat ccggcgatgc tggcgcgttg aattttacat
     5401 cccgtacgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gttcagtacg
     5461 gaatttagct tgacggtgct atccagcatt aaatactggc aatactctca cgcaaaatcc
     5521 gcagcaccgt ctcttcaccg ctgcgattcg ggttaatgcg aatcgcacaa tgttctgatt
     5581 gtggattcgc ctggcgaaac gttccggaaa ggcgataaaa gagcggcggg atttcatatt
     5641 tcgactctgc acccaccggg taaggcaagg caccgcgctt ttgcgcctct tccagcactc
     5701 tggcggcaat cggctgatga aactcgacaa tcaacacctt cgactgcgca ttagcaatca
     5761 ccgcgctttt cacttccggc accgcaccac cgttaagcaa agccagcaac cgttcagata
     5821 cccctgcctg caccgcgtgc atcactggcg caaacaccag accacgcaat acttccagcg
     5881 cctgtgcgcc ctggatctgg ctaccgccgg agtaaagcgt ggcgcgaata cggttgataa
     5941 catcagcatc gccgaccact gcaccaacac cctctggccc aaatagcttg aagcaggaaa
     6001 atgtcgagac attcgcgccg cattcacagc cgattcgcgc caccttcatc accgcatagt
     6061 tgtcatcggt taacgctgga acacctgccg cgcgcaacgt tgccagcaca tctgccagca
     6121 cgtagctgtc ctgcggctgc tggcgcgtat gctgcaccag cgccgcatcc ggttgttgct
     6181 cgtcgacgac ctgcttcagt gccgacaggt cattgaaatc aacagtaata agcgtcagcc
     6241 ccatctgctc aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac
     6301 gctgccccgg tttgagcaaa gccgccagcc cggcgcgaat cgcgccagtc cccgcgccct
     6361 gcaccagcgc cgcagcctgt gcgtgaaatg catcagccag cacctgctcc acccgttggg
     6421 taatacgcgg ttgattcaga cctggagtta accctaaatc accgccagta agaaactcgc
     6481 tgccggggaa atggcgacaa atgctatcca ccagcgcaaa ctgcttttgc tgcgcctcaa
     6541 taatcgtcag gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa
     6601 caggccaagc cagtaaagaa cattcagcag aataccggtg atcattactg ccacgaccgg
     6661 tgccgccatt ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc
     6721 gatagagaat ccggtataac ccgccatctt aattgctgcg aaaatcgaac cgaccagcag
     6781 cgccacttcc atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga
     6841 cgggtagcgt cccagccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac
     6901 cactgcgcct aataccgccg caaccatcgg atttggcgag agatagccca ccgcataaac
     6961 aaaggtaaag cccgcaactg catacacgcc agtggctaac gcggtggtgg caatcaacgg
     7021 cacaaaaccc agtccgcgca taaattctgc cagagccgcc tgattaatca gcgtttgcga
     7081 ttgttccggc gtcacgcctg cggaatacgc tttctccagt gtgaagatcg aaacttcgct
     7141 accagcaaaa atcttcatac tggcaacggc ggcaatcaat gctcccacga tggcgatata
     7201 aggtaagttt ttgataatcc gcgacgtgcg ctcttcaaac accgaaagcc cgctggcatc
     7261 gatgtcattt tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gcagcatcac
     7321 catgccaata aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgactac
     7381 cacacgggtc atcagaacaa ccacggcggc gaccagactt tgcttccagc caaactggta
     7441 gaaaatcgcc accagcggga acagtgcaaa agcagaaacc accggcgagc ttaattcccc
     7501 gaggctacct aatacatcca ccggcagcgc ggtcagcagc tggtttactg gcaacaggca
     7561 agtaaggatc aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc
     7621 cagtacgccg aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt
     7681 cagggtaaaa gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact
     7741 ccccgcctcg cgacgattca tatagccttc aatcagttgc ggcaggatcg ggcggatgcc
     7801 gtcatgaaaa acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc
     7861 caccacgata atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc
     7921 agcatcggaa tcgcgtgttc gacgtgctca acggaaaggc caaacgccac tttcccttca
     7981 gcgatcattt tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa
     8041 cttttgttat agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta
     8101 cacgcaccga tgtagtaatc cagttgcccg gatttcactt tcattgccgc ttccatgtcg
     8161 ttgtgaatga aaacttcaaa actgccagga gccgtcgctt caatggtttt tttaatctgc
     8221 tcacgctgta agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg
     8281 agagggattt tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa
     8341 ttgcggaata aaggtggtca ataaatagtc atagccataa ccaccattgg cttttaaatg
     8401 ggagcggcgc gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag
     8461 cgcgtggagc atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc
     8521 gaactgcacg tacgcgccga gatcgatcat cttcaaaatg ttgtcgagat tgtctttcag
     8581 atcgcagtga ccaacggtga cgcgcgaaag atcaactccg tgggcttgca acagagccag
     8641 ttgttccagc cccatggtgc tgaacgaagt atgcgtggag atcgggcgtc cggtctggtt
     8701 atgcgccagc gcagcggcaa taaacacctt ctcttccagc ggcgtaatct tcccttcgct
     8761 agagccgatc tccgcgataa tcccggcttt caggtcggtg ccatcgatac cctgttcaat
     8821 ttcatcgacc atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gctccgggaa
     8881 aaaagcgtcc tggtaataac cggtgcaggc caccacgttg atccccgtct cgcgcattac
     8941 atcgagcata aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg
     9001 cacgccccgg gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg
     9061 gcagtccacg ttgtttttaa agccggagag atcaatatgc agatgctcat gggccagggt
     9121 gtaacccgtc ggatcaatac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa
     9181 gagcgaccat tttgtggcgg tggcgcacgg aaaaattcac acacggtagc ccccacatca
     9241 gaaagcgtgg tgcgtacacc gagttgcgtg tggaccagcc cttgctgata aaccagcact
     9301 ggcaccactt cacgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc
     9361 atcacgacca ggcaatcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca
     9421 acgacctgca aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt
     9481 tcctggatat tgatgcaaat aaacgccgtc ggatgcgtgt taaattcgtc gagggtgata
     9541 tccataatcc gctggctatc caccaggttt tgccagctca cgccataagg attgctgacg
     9601 atatctgcca ccttacctac cagcacggtt ggcacgcccg cctcatacag tttttgcggc
     9661 acctgcactt tttcatcaac accgtagccc atatgcacga cctggaagcc gttgtcataa
     9721 gcgccagaac gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg
     9781 agaatacgct gactgtcggt taacaagccg ccaaatgtaa tgacccgacc gacctgtacc
     9841 tgctcacgca cgatacgacc aattttgatc gcgtcgtcaa aagagatcac agagagattg
     9901 gcggtaatgt tatagacctg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc
     9961 tgattgaccc acagaaattg cagatcatcg ccacggcgct ccacctgcca gccagcggaa
    10021 actaatgcct gctcaacacg gccaatcaca tcgcgaaagg gcatccgcag cggcggtaac
    10081 gggcgcgtgc ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc
    10141 tctgccaccc cccaggttgc ggaatctgac ggttgcatat cacctggtgc ataacccaat
    10201 gcgttgatta gccccagctt ctccagcgct ggtagctgca aatgcggcaa ctggctcagg
    10261 atgtgaccac aggtattcgc cccggcatct tgcgggcgca ccagcgtgac atctttcatt
    10321 gcccctacgc caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag
    10381 agtttccaag actgtcataa cgaccgacga ttttcggttc gccacggtga ataccggaca
    10441 ccagcaccac atcgctacga gtgacaaaaa tctgcgtgcg aaaacagagc actactgcac
    10501 tgcttaccgg aaactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag
    10561 ttttgagatt ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg
    10621 catgaccacg acggtagtaa ccgccgccgt agcagtagct gtcgccacgg aaatgatggg
    10681 agatttcact taaccaaagc atcgctatat attcaggctg atcgccctgc tggtttgccg
    10741 gaatagtgcc cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tccgccagca
    10801 tcggcagcga agtgcagctg gtcgcagaag gcgcgttcag ttgctcaatc gcaataccag
    10861 atttcgccag ttgatcccgt gcttgtacca gcgtgtgaag attcggtgtc ggcaaaactt
    10921 tcccggcagc ctcatcccaa agcaggcaag ggaaatgggt aagtccggct aaatgcagac
    10981 ccggcagttt cttaatttca gcaaccacct cgtgcagcga atgctgtaca aaaccgctct
    11041 cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca aactgcacac
    11101 gtccggcctt caccgctgcc gcagaaactt cccgcgcttt gtcgagagta aacacggtga
    11161 tgacgtcggt gccctgttca acggcgtcag caacctgatg acaagggatt tgtaccagat
    11221 gcccctgatg cgccaccgac aaaccagcgc ggcgcatgac tcgcgcctct ttgtaatcca
    11281 ccgccacaat gccgctgtaa cctaatgcca gcaatttttc cgccagccat gggttacggc
    11341 caaactgctt ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc tcaatcagtc
    11401 gcttaccgtt ttccagtacc tgatccacgt cgatcaccca gctgtccggg gcgatcttgc
    11461 cctgctgcca caaacttagt acggcggaaa tcagcgccgg gttctggcgt ttcaatgctt
    11521 ctacaaacat cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttttgaata
    11581 tttaggttgt gcgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca
    11641 taatccataa aggttggcca gcaaatagcc ttcttcacac gggttaactt ccagtgcgaa
    11701 ttccttcaac aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag
    11761 ctctgccagc aattcgttat ccagcggctc tatttcttca ccacggcgac tgcgcatcag
    11821 tgcactcgcc atatgtgtca tcgccatcgt tccttgctca ctgcgcaccg gcagatggca
    11881 ctctgtttcc agtacgttga cgacctgcat catgcctttg cagatgtcct tatcaataac
    11941 gcctgcctcg caaagcaggt tgagtctggt ttccatatca gatgcctttc gtttaataac
    12001 tcggttcctg ttccccactc actacccgtt gttgatgggc gagcagggcg tgtttatcta
    12061 caacggcacg cagcagttgt tgcattgggt aatcatcgac tcgcgtcaga accacggctt
    12121 cggtggcctg taaaaatcgc ggatcgtctg tgagcggcgt cgcctctaat cccagcatgg
    12181 tcagttcgtt ttccgccacc acgttccaga tcaccgcatc gacatcgcct ttaacaatgc
    12241 gttgtaaact ctcgtgataa gagagatcga cacgttccac atcactatcg ccaaaaaaga
    12301 catcggtcat gattttctga tccgccgaac ggttatccag ccccacgcgc ttcacgtttg
    12361 cggactcgcc tttacggcaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact
    12421 ccagcgcgag gcaaaaccct ttttgcgtga gataactttc cgccgccagt cgggaaacca
    12481 ccgccatgtc atacacgccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatat
    12541 gcgcatagta aaaaggaatg ccatcaaact gggctttcaa tccgctcgct aaaccttcgt
    12601 acaagcgggt atagggcaaa ggcatcgcac ataccacgtt gttgatatcc acatgactca
    12661 gcaatgcttt gttatccatc tcgaccagat aactgccatt gcgcccacgg cgttcaatcc
    12721 gtatcgctcc gcttgattcc agcgttttca acgcggcctg cgtcaggcca acggatgaac
    12781 ggcattcagt tgccagctca tctatggttt tcaacctatt accgcatttt tcacccaaca
    12841 aataacgggc cagcgtcgtg ataacgacgc cttctttttt gataaacgtt cgacgcataa
    12901 tatattttca ataaaatgaa tgtttatatc ttcattaaaa tgaagataag tggcaactgt
    12961 gagatgtgga aacggcgagg cgcttcacat tttttcggat tattgcaaat gaggataaaa
    13021 aaaccgggtt tccccggttt cagagtgatg acgaagtgat ttattcgaca atgcgagcaa
    13081 agggtaattc ccactggcgc ttgcctgctc ccctcaccct aaccctctcc ccaaaggggc
    13141 gaggggaccg tccgagcgtt ttgatattac ggcttcgcca caaaaccaat cgcttcgtac
    13201 accgctttca gcgtacggga agcgtgtaca ctggcttttt ctgcgccgtc tttcatcacc
    13261 tgttgcagga aggcttcatc gttgcggaaa cggtgatagc gttcctgcaa ttcagtcagc
    13321 ataccggaaa cggcatcagc cacttcacct ttcagatgac catacatctt gccttcgaac
    13381 tgtttttcca gttctggaat gctctggccc gttaccgcag aaaggatatc cagcaggttg
    13441 gaaacgcccg ctttgttctg cacatcgtag cgaactaccg gcggctcgtc ggagtcagtg
    13501 accgcgcgtt tgattttctt cactaccgat ttcggatctt ccagcaggcc gataacgtta
    13561 ttgcggttat cgtcagactt ggacatcttc ttggtcggct ccagcagcga cattacgcgc
    13621 gcgccagatt tcggaataaa cggctccggc accttaaaga tctcgccata cagcgcgttg
    13681 aaacgctggg cgatatcgcg actcagttcc aggtgctgtt tctggtcttc acccaccggt
    13741 accagattag tttgatacag caggatgtcc gcagccatca gcaccggata gtcaaacaga
    13801 ccagcgttga tgttctcggc ataacgcgca gatttatctt taaactgcgt catgcggctc
    13861 agttcgccga agtaggtata gcagttcagt gcccagccta actgcgcatg ttctggtacg
    13921 tgggactgaa caaaaatggt gcttttctca ggatcgatac cacaagccag atacaaggcc
    13981 agcgtatcca acgtcgcttt acgcagcttc tgtgcatcct ggcgcacggt gatcgcgtgt
    14041 tggtcaacga tacagtaaat gcaatggtag tcatcctgca tgtttaccca ctgacgcagc
    14101 gcacccatgt agttaccaat ggtcaattca cctgagggct gtgcgccact aaaaacgatg
    14161 ggcttagtca tttttcgatt cctgattttc gctatgcgga agccctaatg cgggcagaag
    14221 gtcatttata gactgataaa ttacatcagg ctggctgaga tcgatagcct cgccgtagtt
    14281 atatccgtag gttaagccaa ctgatgggca acctgccgct tttgccgcct gaatatcatt
    14341 gcgtgagtcg ccgacaaaca gcatctgttg tggggcaatt cccatccgct cagccaccag
    14401 taacagcggg tccggatgcg gttttttgtt ttgcacatca tcgccgccga taaccacgct
    14461 aaagtatttg gcaatatcta aggcttcgag cagcggcgcg acgaacggcg tcggtttgtt
    14521 ggtgaccagg cctagcggca ggcctttagc ctgcaacgcg cccaacgtat cggcaacgtg
    14581 cgggaacaaa aacgtcccct cttcggcaac ctcgccatag tagcgatcga acagtttacg
    14641 cagaatacgt acctgttctt ctgccggaat gtcgtcatca acgggcggtt tacccattgt
    14701 tttacgctga gtcgcacgtt cctgacgcgc ccaggtcaat gcgcgctcca tcagaacatc
    14761 tgcgccgtta ccaatccagg taataacgcg ttcttcacct gcgacgggca actccagcgc
    14821 atacagcgcc atatctaccg cagcagcaag accgggagca ctgtcgacca gcgtaccatc
    14881 aagatcaaaa gcgacgccgc gaatatcttc aaacttattc atgacttacc tttgccagtt
    14941 cactgcgcat ttcatcaatg acttttttgt agtctggctg gtcgaagatt gccgaaccgg
    15001 cgacgaacat atccgcgccc gccgcagcga tttcgccaat gttgttcacc ttcacgccac
    15061 cgtctacttc cagacgaatg tcaaagccag actcgtcgat acgacgacgt acttcgcgca
    15121 gtttatccag tgtttgagga atgaacgact gaccgccgaa accaggattg acggacatca
    15181 gcaggatcac atccagctta tccatcacgt aatccagata gctcagcggt gtcgccgggt
    15241 taaataccag tcccgcttta cagccatttt ctttaatcag ttgcagcgtg cggtcaacat
    15301 gctcggaggc ttctggatga aaggtaatga tgctggcacc agcggcagcg aaatcaggca
    15361 caatgcgatc gacaggtttc accatcaggt gtacgtcgat aggggcggta atgccgtagt
    15421 tacgcaagga tttcagcacc attggcccaa tcgtcagatt gggaacatag tggttatcca
    15481 tgacgtcaaa atgcacgaca tcagcgccag ctgccagggc ttttgcggta tcttcaccca
    15541 ggcgggcaaa atcagccgac agaattgagg gggcaatcaa atactgtttc atccgcttct
    15601 ccttgagaat tattttttcg cgggtgaaac gactcctggt ttgtacaaag ccagcagttc
    15661 gtccaccttt ttacgtgtgc cgccgttgct gcttatactg cgtcgaactt tgacgacatg
    15721 caattttgcg cgctgatacc actcacgcgt taacatcgta tcgtgattgg agatcagcac
    15781 tggaatatgg cgctcaacca gaccttcggc gatctccgcc agatgcgctt gttgttcaag
    15841 cgtaaaactg tttgtgtgat acgccgtaaa gttggcggtc gcagacagcg gtgcataagg
    15901 cggatcgcaa tagacgacgg atgcatcatc tgcgcgcgcc atgctatcgg cgtaagactc
    15961 acaatagaaa aaggcattct gcgctttttc agcgaagtga tacaactctg cttccgggaa
    16021 atagggtttt ttgtagcggc caaacggcac gttaaactca ccgcgcagat tgtaacgaca
    16081 caggccgttg taaccgtagc ggttcaaata taaaaacagt accgcccgac ggaacggatc
    16141 ctggcttttg ttgaactctt cgcggaactg atagtagacc tcggcgcaat ttgtttcggg
    16201 aacaaacagc tcgcgtgcgg cctgtacgta ctcatcagta cgcatcttca caatgttata
    16261 gagactgatc aggtcgctat tgatatcggc aaggatataa cgagaaaagt cggtgttgag
    16321 aaacaccgac ccggcaccta caaaaggctc aaccagacat tcgcccttgg gcaaatgccg
    16381 tttaatatca tcaagcaggg gatacttgcc ccctgcccac ttcaaaaaag cgcgattttt
    16441 cttcatgctg actaactaat tacaccttct ccggctgtgg agaaagctcc gacagcatcc
    16501 agcgcttcag gcaactcccg caacgataac ccattgcggg agataacctt gattacttca
    16561 gatcggcctg tacctgacgc agcggtttcg cccacgggtt tttggcctga acatctgctg
    16621 gcaatgtaga taccgctttt ttcgcctctt ctttcgaagc gtacacgcca gaaaccagga
    16681 cataccacgg ctgaccatta cgcgtcgttt catagacaac gtagtttttc agattctctt
    16741 tcttcgccca accgttcagg ttgtcgtagt tagaggaact gctcagctgc agagtgtaat
    16801 ggctggacgg tgccgatttc aacgaaccaa cattacctgc ggtcttccct ccagcggctg
    16861 gtgttgccgt ggtttgcgcc ggggatgccg tctgtactgg agccgtagtc gccgtctctt
    16921 ttggtgctgg cgcagaagtc gcagccggtg ccttcgtgct cgccacagga gcagctggtt
    16981 cagtacgctt cggcgtctgt gctaccggct tcggttccgt tttcacggtt gcttgcggtt
    17041 ttttcggttc aatcaccgcc tgctgacgag ctggacgcgt agtggccgga cgttcagcag
    17101 tttgcgtttt cgccgtgtca cgcgatgcat tgccattgcg tactggcgcg acagttgctg
    17161 gttcagtcgg caatgtagaa ttgaccgcca cattgttcaa ctgttgctga ttttgtggct
    17221 gggtcagcgc attgttcagg tcaccctgca cttcaacacg ttgttgacca tccgttgccg
    17281 ccggggtttg cccttgagtc ggcgtagaag agatcggtgg cagagaaaca tcctgctgag
    17341 tattttccgc agacgtggtt cccggcgctg gctgcacccc attcgcctga tcggtcgcat
    17401 tgcctgcaag atcaatactc ttctcgccag acgcggtttg atcgctggaa gaggtcgagg
    17461 gggcttttag cgcagaaccg ataccgatga tcaacagcag tagaaccaga atgccgacgc
    17521 ccatcatcat atactgacga gaagcgggtt tactggctgc ttttttgcgc ttacgcggac
    17581 gacgctctac gcgctcttca tccacggttt catcttcgga ttcgtcaatt tcttcttcga
    17641 tttccggttc ctcattgcgc tctttttgcg cacgagtcgg acggcgatcg tcagtgtcat
    17701 caagttcaat atcatcaaaa ttgatctgcg gttcgccacg ttcagtacgc tcagaacgtt
    17761 cagaagattg acgagaacga ccagtacgac gatcgctggg atcgggtttc agctcgtctt
    17821 ctggtttgaa ttcatccatt taacacccca ctaaaaggtt aatgcttacc acattgcaat
    17881 taacctgaag ctaatagacc gcttgataag cggcctgacc tttcttgttg ttacgctgat
    17941 tgactatcgg caatggcgtt aagaacaagc tcgtgcgaaa cgccgctgcg aacttcactc
    18001 ttaccaattg ccaacggaag aattaagcgt atctctcccg caaggacttt cttgtcacgc
    18061 agcatatgcg gtaaatacgc ctgcgcggac atttcgcgcg gcccattgac cggtaaccca
    18121 gcccgcttga gcagggttat aatacgctgc gtttcggcag aactaaactg cccgagacgt
    18181 tccgacgtcc gcgccgccat caccataccc gcagcgaccg cttcaccatg taaccaattg
    18241 ccatacccca tttcagcttc aatggcatga ccaaaggtgt gtcccagatt cagtaaagca
    18301 cgtaacccgg tttcgcgctc gtcggcggcg acaacttctg ccttcagttc acaacaacgg
    18361 cgaatacagt acgccattgc cggaccgtcc agacgcaaca acgcatccag attctcttcc
    18421 agccagttaa aaaacgcacc gtcaagaata atgccgtatt tgatgacttc tgccagcccc
    18481 gacgctaact cacgcggggg aagcgttttc agacagtcga gatccaccac cactgaagca
    18541 ggctggtaga acgcgccaat catgttttta ccgaggggat ggttgaccgc agttttgccg
    18601 ccaacggagg aatcgacctg cgacagtaac gtcgtcggga cttgaatgaa acgaacaccg
    18661 cgctgataac tcgccgccgc gaagccggtc agatcgccca ctacgccgcc gccaagcgcc
    18721 accagcgtag tatcgcgacc atgcggcttt tgtaacaacg ccgtaaagac ggtatcgagt
    18781 acagccaggc ttttatactg ctcgccgtca gggaggataa cgctatcgac gttaacaccc
    18841 gcctgttcaa gtacgccgcg gaccttatcg agatacagag gagccagggt ttcgttggtg
    18901 accaacatga cctgctcgcc cgatttcagc ggtaagaatg aagctggttc attaaacaaa
    18961 ccagatgcga tggtaattgg gtaactacgt tccccgagag taacgacaat cctctccata
    19021 acgcgacatc caccttaatt actgtacccg cagacgagtg tatataaagc cagaattagt
    19081 tgctttccag catgtgaata atctggtttg caaccacttt agcgctttga tcatcagtac
    19141 gaatggtcac gtcggcaatc tcttcataca gcggattgcg ttcattggcc aacgcttcca
    19201 gaacttcacg cggcggtgtt tcaacgtgca gcaacgggcg ttttttatca cgctgcgtgc
    19261 gtgcaagttg cttttcgatg gtcgtttcaa gataaacgac aacgccacga gcggaaagac
    19321 ggttacgcgt ttcacgggat ttcacagagc cgccgccagt agccagcaca ataccctgtt
    19381 tctcggtcaa ctcattgatg accttttctt cgcgatcgcg gaagccttct tcgccttcta
    19441 aatcgaaaac ccagcccaca tcagctccgg ttcgtttctc aatctcttga tcggaatcgt
    19501 aaaattccat attgagttgt tgagctaact ggcgcccaat agtgcttttt ccggcaccca
    19561 taggcccaac cagaaagata ttgcgtttct ctgccatttt ttcggtacta ctaagactat
    19621 tcgttaatga taaacccgct tcgctcagag agcgccgcag gacatgaact gaaacctcat
    19681 aagatattgc gagagtcaga ctgaaaatta tctcaatact ccagcgggtt tggcaactga
    19741 ataaatcacc catccctctg catatctggt cgctgcaagc gcgctgcctt gctaccaccg
    19801 ctctggcgat aaatcaccgg gtaagattag cgtaaaaaag acagcaaaat gccgcctgaa
    19861 tgataaatca tcatcatgga acgcaggcag cgaagagcac caactctcaa atcggtactc
    19921 cttgtatgct aaatacctgc gcgcgtcaaa tagatgaaac acgttcagca taaaaacatt
    19981 accgctttta cggctgttta ctcactggaa accagtcgtg gcgtgataaa caccactaac
    20041 tcgcgtcgtt catcttcttt tccgtcatga cgaaataatt gcccgaacca gggaatgtcg
    20101 ccaagcaacg gtacgctatc ctgacccgat ttatttttac gggtaaaaat gccgcccagc
    20161 gccaacgttt ctccgctttt gacctcgacc tgcgtttcga tctcctgctt atcaatcgcc
    20221 agcacttcgc catcggcctg ctgtagcacc tgccccggaa cgttctggct gatgtgtaat
    20281 ttcagccgga tgcgaccttt ttgtaacacc gtgggcgtga cctccatccc caggacggcc
    20341 tctttaaatt ccaccgacgt cgcgccactt tccccgctgg aaacctgata tggaatttcg
    20401 ctcccctgtt taatgctggc aggctgaaga tgtgaggcca gcagacgcgg actggcgata
    20461 atatccagct gctgtttttg ttcgagcgca gaaagctcaa gatccagcaa gcgtccgttg
    20521 atgcgcccaa tgttaaaacc gacatgcgtt gtcgccgtcg ctacggagag gtcgctacca
    20581 agcgtggtga cttgcccaac gccaccagcg tgttgcgcat cggccagcgt ccatttcacg
    20641 cctaactcac gcaaactttt ttcattaatg gtgacaatat gcgccgacag ctcaacctgc
    20701 ccgaccggca gatccatttg cgctacccac tgttcaagcg cgcttaacgc cgttttgtta
    20761 tctcgtagca aaaggcgatt ggtgcgttta tcgacggtca tactcccttt ggcactcagt
    20821 agcttctccc ccgctttcgc cagttctccc gcgtcggcgt attgcagggt tatactgcga
    20881 ttttccagcg gcagatttgc ctgcgcccgc gcctgctccg cctcctggcg ggcgatattg
    20941 ttattctgcc aggcaatgga atgcactgag agaatgttgt cttcctgccg cgttatcagt
    21001 ccggcgcttt tcactacagt ttgtagtgcc tgcttccagg gaacatctgt tagatgtaac
    21061 gacaccgtac cgctgacgtc tggcgacacg accaggttca acttctcctg ttcagccagc
    21121 gcctgcaaca cctgagctac cggaacgtca tccaccatca gcgtcacttt ttgcggcttt
    21181 gccgcctgta cgccgggtat cagcatcaac agtagtgcgg ctatccattg cttcatttgt
    21241 atctccttgc cgttgccaca accattgtgg cggttcgcag tttgtcccgg ttcccagcgt
    21301 tagtgcgtct ggcgtcagct gtaaaattgt ccagccgttt tccagcacat cgttttgctg
    21361 cacccgtcgc catttctttt gcccgtcttt tattacaccg atgatgtgct cgcctcgccc
    21421 taccatcccc tgatagcgcc actggctaag ttcgctaatc cggcatagat cttccggcgg
    21481 tttaaaaggg tcacgcatac cggttaaaag gcacaatgca atacctgcca acaaccagcg
    21541 tttaaccctc atgcggtgtc tccagttgta gcgtgaacaa aagatcatca ccttccacgc
    21601 ttaacgaaaa acggctcacg ctgacgttgc gctctgccag ccgtgtaaat gccgacggca
    21661 ctgcttccca cagcgttttc aacgccaact cacctccctg cgcggatgga tgccaggaaa
    21721 ccagttgcgc gccgggcaac tgaaaatcca gtggcgaaaa gggcagcgtt ttttcttcgc
    21781 tgaagggagt ggtgtcaacc aggcgataca gtgtggccca ctgctggtga tgacttgccc
    21841 gtagtcgaat taatgcgtcg cgctcttcat ggtgtgtcga tgacagaaaa atgagcgtaa
    21901 ctaacatcag caaccagaat gcccagcaaa actggcggag gcggggtgat gtggcgaacc
    21961 accagtcaaa gaacatgttc atcgctaacc ttccttgtta actgatactc aaattgccag
    22021 cgtccctgcg catcctgctg cgtggctccc cgctgattga gatgaaaaga cgcatcctgg
    22081 cggagtgacg tttctagtgc gtttaacgcg gtaatgcttg ttgtcagccc cttgatctcc
    22141 agcgttccct gctgccagct tatcgttgtc agccaggcgt gctcgggtaa aagcgccgcc
    22201 agtgcttcca gcgcagattg ccagtcgcgg gtaaattgtc gctggcgctg ccgttgcgaa
    22261 cgctgttcgc gtaactgctg ttgctccagc aaacgtggct tcgttatctg taagctgcgg
    22321 gcgagttgtt gttccgcctg aagcaaaact gcgtctatgc gcgcttcggc gctgcctgtc
    22381 agacgcagta ttagcgttat cccgacggcc agcagcagag gcgcaacgaa catcagcaac
    22441 cagaaacgca gaaaagcggt ccggcgttgc tgtcgccagg gcaaaaaatt aattggcggg
    22501 ttcatcagta cgccttccca agcgccagcc ccagcgcgat ggcaaagtct ccaccaggcg
    22561 gcggtagcgg cggctgacga acagaaacgg cctcccaggg gtcaaatccg ccttcgccac
    22621 atatcgcgac gctgtcggga tcaacggata acgctgccgc cagctccttc gcgctagtca
    22681 tccccaccgc cagtttgcgc ccccagctat agcgcgtcgc ccacagccac tgttcgttat
    22741 cacgccaggc cagacattgc tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac
    22801 tggcatccgg ggtgatcgca ctaacatgaa cacgcaacct ttctgccaga gtaagcagcg
    22861 ttgccagctc tttgctttgc gcggcagtca cgttataggc gggactgagt gagtcttcgc
    22921 tgtaatcgaa acgcagggag tccggatcca tatccagctc gcgggccatc gtccctgaca
    22981 gccaggccgt ttgctcccgc tcaccaaggg acatcgacgg gcgcggaaat gaccgctgta
    23041 atgtgcgact ggcgggaaac gccaacataa tgtgatgacg ctgcggcagt tcgcgactcc
    23101 acggtaacaa cgttttagcc agctgctgcg catcaacaat ccgcccatct ttgataatgt
    23161 cgttctccag cggcaaccgc caccagcgtt gcaaaaagca ttcttttgct ccccgcacga
    23221 tcgcaaccgc taccgcttct tgctgttgta aatgcaaacc aatttgccag atcttaaatg
    23281 ccattgtgat gctctcctta tcacccgtca ctctgacggg tatatcaatg cgtctggctt
    23341 gcctttatac taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa tgggaaattt
    23401 ccagtgaagt tcgtaaagta ttttttgatc cttgcagtct gttgcattct gctgggagca
    23461 ggctcgattt atggcctata ccgctacatc gagccacaac tgccggatgt ggcgacatta
    23521 aaagatgttc gcctgcaaat tccgatgcag atttacagcg ccgatggcga gctgattgct
    23581 caatacggtg agaaacgtcg tattccggtt acgttggatc aaatcccacc ggagatggtg
    23641 aaagccttta tcgcgacgga agacagccgc ttctacgagc atcacggcgt tgacccggtg
    23701 gggatcttcc gtgcggcaag tgtggcgctg ttctccggcc acgcgtcaca aggggcgagt
    23761 accattaccc agcagctggc gagaaatttc ttcctcagtc cagaacgcac gctgatgcgt
    23821 aagattaagg aagtcttcct cgcgattcgc attgaacagt tgctgacgaa agacgagatc
    23881 ctcgagcttt atctgaacaa gatttacctc ggttaccgcg cctatggtgt cggtgctgcg
    23941 gcacaagtct atttcggtaa aacggtcgac caactgacgc tgaacgaaat ggctgtgatc
    24001 gccgggctgc cgaaagcgcc ttccaccttt aatccgctct actcaatgga tcgtgccgtc
    24061 gcgcggcgta acgtcgtact gtcgcggatg ctggatgaag ggtatatcac ccaacaacag
    24121 ttcgatcaga cacgcactga ggcgattaac gctaactatc acgcgccgga gattgctttc
    24181 tctgcgccgt acctgagcga aatggtgcgc caggagatgt ataaccgtta tggcgaaagt
    24241 gcctatgaag acggttatcg catttacacc accatcaccc gcaaagtgca gcaggccgca
    24301 cagcaggcgg tacgtaataa cgtgctggac tacgacatgc gccacggcta tcgcggcccg
    24361 gcaaatgtgc tgtggaaagt gggcgagtcg gcgtgggata acaacaagat taccgatacg
    24421 ctgaaggcgc tgccaaccta tggtccgctg ctgcctgccg cagtcaccag cgccaatcct
    24481 cagcaagcga cggcgatgct ggcggacggg tcgaccgtcg cattgagtat ggaaggcgtt
    24541 cgctgggcgc gtccttaccg ttcggatact caacaaggac cgacgccgcg taaagtgacc
    24601 gatgttctgc aaacgggtca gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg
    24661 gcacaagtgc cggaagtgaa ctcggcgctg gtgtcgatca atccgcaaaa cggtgccgtt
    24721 atggcgctgg tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc cacccaggca
    24781 ctgcgtcagg tgggttccaa catcaaaccg ttcctctaca ccgcggcgat ggataaaggt
    24841 ctgacgctgg caagtatgtt gaacgatgtg ccaatttctc gctgggatgc aggtgccggt
    24901 tctgactggc agccgaagaa ctcaccaccg cagtatgctg gtccaattcg cttacgtcag
    24961 gggctgggtc agtcgaaaaa cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac
    25021 tacgctgcag aatatctgca acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa
    25081 tcgctggcgc tgggttcagc gtccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc
    25141 atggcgaacg gtggcttcct ggtggacccg tggtttatca gcaaaattga aaacgatcag
    25201 ggcggcgtga ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgcga tattccggtg
    25261 atttacggtg atacgcagaa atcgaacgtg ctggaaaata acgatgttga agatgtcgct
    25321 atctcccgcg agcagcagaa tgtttctgta ccaatgccgc agctggagca ggcaaatcag
    25381 gcgttagtgg cgaagactgg cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg
    25441 gcattcctga ttaagagtgc tttgaacacc aatatctttg gtgaaccagg ctggcagggt
    25501 accggctggc gtgcaggtcg tgatttgcag cgtcgcgata tcggcgggaa aaccggtacc
    25561 actaacagtt cgaaagatgc gtggttctcg ggttacggtc cgggcgttgt gacctcggtc
    25621 tggattggct ttgatgatca ccgtcgtaat ctcggtcata caacggcttc cggagcgatt
    25681 aaagatcaga tctcaggtta cgaaggcggt gccaagagtg cccagcctgc atgggacgct
    25741 tatatgaaag ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc tccgggtatt
    25801 gtgacggtga atatcgatcg cagcaccggg cagttagcta atggtggcaa cagccgcgaa
    25861 gagtatttca tcgaaggtac gcagccgaca caacaggcgg tgcacgaggt gggaacgacc
    25921 attatcgata atggtgaggc acaggaattg ttctgattaa aaaggcgctt cggcgccttt
    25981 tcagttggct gacaaagtgc atttgtttat gccggatgcg ggtgaacgcg ttatccggct
    26041 aacaaaatcg tgaaaaaatc aatatattgc aggaaaccca taagcctgat aagcgttgtg
    26101 catcaggcaa acttcacgca tttacactcg cccctgccct ttcaaccatt cgcgcacgag
    26161 gaacagcgcg ctgacattac gcgcttcgtt gaagtcaggg tcttccagca aatccatcat
    26221 atgcgccagc ggccagcgca cctgtggtag cggctctggc tcatcgcctt ccagcgattc
    26281 cgggtagaga tcttgcgcga ccacgatatt cattttgctg gaaaagtaag acggtgccat
    26341 gctgagcttc ttcaaaaaag tcagatcgtt cgcaccaaat ccaacctctt cttttagctc
    26401 gcggttagcg gcttcgtaga cgctttcacc cggatcaatt aatcctttcg aaaaacctaa
    26461 ttcgtaggat tcagttccca ctgcgtattc gcggatcagg atcaggtgat cgtccacaat
    26521 cggcacaatc atcactgctt cccggttggt tggacgcatt cgttcataaa cacgccgcac
    26581 gccattgctg aactccagat ccacgctctc gacggtaaac agtcgggaac gggctacagt
    26641 ttcaacattc agaatggtgg gtttttgtaa tgatttgctc atcgtgggat ctatgctgtg
    26701 aaatcagcgg ttattgtgcg atatcggaca cgctttcggc aatgtgaatt gcatgttatt
    26761 tacatttatg taacttaata aataatcgtc ctcaaatcaa attaaaagtc aataggttga
    26821 aataactcca ggaatttgct gatattccgc cttcagaggg tttgctatga tcagcggtta
    26881 ctgtgatgtg cttaatgatg ctcaagttaa actccacgct tgccgatagc caaccgcaga
    26941 atcatgtatt gtgtctggtg cgactgacca cgcctgacag actaagtaag atggggaaag
    27001 catgagcacc attgtgattt ttttagctgc tttgctggcc tgctcactac ttgcgggatg
    27061 gctgataaaa gtgcgatcca gacggcgtca gctgccctgg accaacgcct tcgcggatgc
    27121 gcaaacgcgt aaactcacac ctgaagaacg tagcgccgtt gaaaattatc ttgagagcct
    27181 gacgcaggta ttacaggtgc ctggcccaac gggagccagc gcggcaccga tctctctggc
    27241 gctgaatgcc gaaagcaaca acgtcatgat gctgacacac gctatcacgc gttacggcat
    27301 ctctaccgac gatccgaata aatggcgtta ctacctcgat tcggtagaag tccacctgcc
    27361 ccctttctgg gaacaataca tcaacgatga gaataccgtt gaactgattt ataccgattc
    27421 gctgccgctg gttatttcac tcaacggtca tacgctgcag gagtacatgc aggaaacgcg
    27481 cggctatgcc ttgcaacctg ttccgtccac gcaggcgtcg attcgcgggg aagagagtga
    27541 gcaaatcgag ctactcaata ttcgcaaaga aacgcatgaa gaatatgcgc tgagtcgtcc
    27601 gcgcgggctg cgtgaagcgt tgctgatcgt cgcctccttc ctgatgttct ttttctgcct
    27661 gattaccccg gatgtatttg ttccgtggct ggcaggcggc gcattactgc tgctgggcgc
    27721 aggtctgtgg gggctattcg cgcccccggc aaaatcctcc ctgcgagaaa ttcattgtct
    27781 gcgcggtaca ccccgtcgtt ggggattgtt tggcgaaaac gatcaggaac agatcaacaa
    27841 tatttcactc ggtattatcg acctggtcta tcccgcacac tggcagccat acattgctca
    27901 ggatctcggt caacaaaccg atattgatat ctatctcgac cgccacgtag tgcgtcaggg
    27961 gcgatatctt tcgctgcatg atgaagtaaa aaactttccg ttacagcact ggctgcgcag
    28021 tacgattatc gctgcgggct cgctgctggt gctgtttatg ctgttattct gggtcccgct
    28081 ggatatgccg ctgaaattca ctctctcatg gatgaaaggc gcgcagacca ttgaagccac
    28141 cagcgtaaaa caactggctg acgctggcgt gcgggtaggc gatacattgc gtattagcgg
    28201 tacgggaatg tgtaatattc gcacttccgg aacctggagc gcgaaaacca attcaccttt
    28261 tttaccgttt gactgctcgc agatcatctg gaatgacgcc cgctcattac cattaccaga
    28321 atctgaactg gtcaacaaag cgacggcatt gactgaagca gttaatcgcc agctgcaccc
    28381 taaaccggaa gatgaatctc gcgtcagtgc ctcattacgt tcagcgattc aaaaatccgg
    28441 catggtattg cttgatgatt ttggcgacat tgtactgaag acagcggatt tatgttctgc
    28501 caaagatgac tgtgtgcgac tgaaaaatgc gctggtcaat ctcggcaaca gtaaagactg
    28561 ggacgcgctg gtaaaacgcg ccaacgccgg gaagctcgat ggcgtgaatg tgttattacg
    28621 cccggtgagt gcagaatcgc tggataacct ggtggcaacc tccaccgcgc cgtttatcac
    28681 gcatgaaacg gcgcgagcgg cacaatcact aaacagtccg gcccccggcg gattcctgat
    28741 tgtcagcgac gaaggcagcg attttgttga tcagccctgg ccttcggcat cactttacga
    28801 ctacccgccg caagaacagt ggaacgcttt ccagaaactg gcacaaatgc tgatgcatac
    28861 gccgtttaac gccgaaggta tcgtcacaaa aatcttcact gacgccaatg gtacgcagca
    28921 tattggcctt catccgatcc cggatcgttc cggactgtgg cgctatctca gcaccacatt
    28981 gctgctactg acgatgctgg gtagcgccat ttacaatggc gtacaggcct ggcgtcgtta
    29041 ccagcgtcat cgcactcgca tgatggagat tcaggcctat tatgaaagct gcctgaaccc
    29101 gcaactgatc accccttcag aaagccttat cgaataacac gtttgcgcgg caggttatgc
    29161 taccctgtcg cgcaaattgc ttcactctgg agatttccct catgcatatc aacattgcct
    29221 ggcaggacgt agataccgtt ctgctggata tggacggcac gttgctcgac ctcgccttcg
    29281 ataactattt ctggcaaaag ctggtgcctg aaacatgggg cgccaaaaac ggggttacgc
    29341 cacaggaagc gatggaatat atgcgccagc aatatcacga cgtacagcat acgctaaact
    29401 ggtactgtct tgattactgg agtgagcaac tgggtctgga tatctgtgcg atgaccaccg
    29461 agatgggacc gcgtgccgta ctgcgtgaag ataccattcc gtttcttgag gcactgaaag
    29521 ccagcggtaa gcagcgaatt ttgctcacca atgcgcatcc gcacaacctg gcggtaaaac
    29581 ttgagcatac cggtctggac gcacaccttg atttattact ttccacccac acatttggtt
    29641 atccgaaaga ggatcagcgg ttatggcatg cggtggccga agctacgggt ctgaaagctg
    29701 aaagaacgct gtttattgat gacagcgaag cgattctcga tgctgccgcg caatttggta
    29761 ttcgttactg cctcggcgtg actaatcctg attccgggat tgccgagaaa cagtatcaac
    29821 gccatccgtc actgaatgac taccgccgcc tgatcccctc gctaatgtga aggagacgcc
    29881 atgaaagaga aacctgctgt tgaggttcga ctggataaat ggctatgggc tgcccgtttt
    29941 tataaaaccc gcgcgctggc ccgtgaaatg attgaaggcg gtaaggtgca ttacaacggg
    30001 cagcgcagca agccgagcaa aatcgtcgag ctgaatgcca cgctcactct gcgccaggga
    30061 aatgacgaac gcacggtgat tgtaaaggcg attactgaac agcgtcgccc cgccagcgag
    30121 gcagccttgc tgtatgaaga gactgcggaa agtgtagaga aacgcgaaaa aatggcgatg
    30181 gcacgtaaac ttaatgcctt aaccatgccg cacccggacc gacgcccgga caaaaaagag
    30241 cgccgcgacc tgttacgatt taaacacggc gacagtgaat aactgtcacc tgcaagagag
    30301 atgattatgc cgcaacatga ccaattacat cgctatctgt ttgaaaattt tgccgttcgc
    30361 ggcgaactgg taaccgtttc ggaaaccctg caacagatcc ttgagaacca cgattatccg
    30421 cagcccgtta aaaacgtgct ggcagaactg ctggttgcga ccagcctgtt aaccgctacg
    30481 ctgaagtttg atggtgatat caccgtacag ctgcaaggcg acggtccgat gaatctggcg
    30541 gttattaacg gtaacaataa ccagcaaatg cgcggtgtgg cgcgcgtgca gggcgaaatt
    30601 ccagaaaatg ccgacctgaa aacgctggtc ggcaatggtt acgtggtgat caccattacc
    30661 ccgagcgaag gcgaacgcta tcagggcgtg gttggtctgg aaggtgatac cctggcggcc
    30721 tgcctggaag attactttat gcgttctgaa cagctgccga cgcgcctgtt tattcgcacc
    30781 ggcgacgtag acggcaaacc ggctgcaggc ggtatgttgt tgcaggtaat gcctgcgcaa
    30841 aatgcccagc aggacgactt tgaccacctg gcgacgctaa ccgaaaccat caaaaccgaa
    30901 gaactgctga ccttaccggc aaacgaagtg ttgtggcgtt tgtatcacga agaagaggtg
    30961 acggtttacg atccgcagga tgtggagttc aaatgcacct gctcgcgtga acgttgcgcc
    31021 gatgcgctga aaacgctgcc tgatgaagaa gttgatagca tcctgacgga agatggcgaa
    31081 attgacatgc attgtgatta ctgcggtaac cactatctgt tcaatgcgat ggatattgct
    31141 gaaatccgca acaacgcgtc tccggcagat ccgcaagttc attaatgagt atgtccggca
    31201 gagagggctc tgccggattt tacgacgtaa tggcctgatg tgctgcgctt accaggcctg
    31261 tgaaataaat cgcaaattat tgttggtcag atacagcgtc gtatccgtta aaagagttgt
    31321 cacttactta agtttatttc cagcgtttca ccatctaccg tcagatcgcg ccacgctttc
    31381 ggcctcgtcc ccagttttcc atccttcgca gctgtgagta atttttcttt cgttatttcg
    31441 ttcggaagta aactcatatc aagcgcgtcc cattcttttc tgtctgagtc aaagccatag
    31501 acaatgactc tgtcatcatt aaaagcaaac aggatccgct ccgcctgacc atcactattc
    31561 agatcttgct cgaccaggac gcaggcgtct ttttcaatac aggtcatcac gttatagcga
    31621 tcctggatta aggccgacca gaatgtcgca tccggtttaa cagaaccagg ggcaattaac
    31681 acattttcgg ctaatacttt ttccgatacc tgttcctgaa gatgttgctc tccatctaac
    31741 gccatcagca gatcccgcgc gcgtttcggg tctttcataa acccggcatc gcttttcagc
    31801 gactcaagcg ccgcacgtcc atagcgaccg ctctgttcga gcatgtagat agttacctgg
    31861 tctggcgtgt ttttgccgct ctgataacgc gccatatgac tgttcacgct aatacgcata
    31921 ctatccagca ccggcgaatt aagaagcacc agtatcacca acaccaataa cgaaaccgca
    31981 aggttcactt tgccctgaag aacaagggga ttttgcccat tacgccagac gatactgaca
    32041 aaatacccca gcgaccacac cagtaacacc agcaccgcca gcgcgccctg caagcggtcg
    32101 acagtccagc cgtactgagc gacccgcagc cataacgccc aggcggcgac gaacacatac
    32161 agcggagcaa ccagcaaagc ggttttaatc aggcaacgca acggccccgt ccagggaagt
    32221 gacgcttttt gcggatcgcg gacaatagcc attaagatca attgcaaaaa ggccagcgtt
    32281 aacagcaacc cggcggcgga gatgtggcga gaaatcgcgc tcaggcccgt aaacggcaga
    32341 gtgatgataa acatcagggt tagcaatgat actaacggca gcaaccccgt ggcgattaac
    32401 gtgaacaact tttgaataga gtcgattaaa cgtgactgtg tgcgcgcgag aatcaccgcc
    32461 agtgcggtaa ccagacctaa tgtaagatac ataaaccagt cggttgcaaa aaagagcgta
    32521 ttgaaaaact tgatgccaac aagtttaaac aactcacccc acagtaaaag caccacccac
    32581 gtcaggccat tggcgagaaa aatcaccagt aatataagta cgttatgcca tactgacttg
    32641 taaaagtagc tatagcggct actgccattg cggatacgaa ggctttgttg tatccacggt
    32701 agcaacaaca ttgccatcaa cagcagatag cagccaaaat cccaaatcgc cttttcagct
    32761 ctccaggggt tcatgccatc agtttgccac ttcagccaac cgctcatccc tgtcgtggca
    32821 ataaacacca gcgccagcca tccccaaagg cgcttctgtt taaaagagat cacggagaag
    32881 agtaaaacgg atgaaaacgc aacggtcgca ggcacgccat aaacaatcca actgtgattt
    32941 tttccggcaa gccaggcgat caacaggtag caaaccagcc cctgaagtaa tccggtagca
    33001 atcatccccc agcgtgttgc cggtgaaagt tcaacgttat ccatgttggt tatccagaat
    33061 caaaaggtgg gttaattatc gcatccgggc agtagtattt tgcttttttc agaaaataat
    33121 caaaaaaagt tagcgtggtg aatcgatact ttaccgattg aatttgcatc aatttcatta
    33181 aggaatgcga ttccactcac aatattcccg ccatataaac caagatttaa ccttttgaga
    33241 acattttcca cacctaaaat gctatttctg cgataatagc aaccgtttcg tgacaggaat
    33301 cacggagttt tttgtcaaat atgaatttct ccagatacgt aaatctatga gccttgtcgc
    33361 ggttaacacc cccaaaaaga ctttactatt caggcaatac atattggcta aggagcagtg
    33421 aaatgcgcgt taacaatggt ttgaccccgc aagaactcga ggcttatggt atcagtgacg
    33481 tacatgatat cgtttacaac ccaagctacg acctgctgta tcaggaagag ctcgatccga
    33541 gcctgacagg ttatgagcgc ggggtgttaa ctaatctggg tgccgttgcc gtcgataccg
    33601 ggatcttcac cggtcgttca ccaaaagata agtatatcgt ccgtgacgat accactcgcg
    33661 atactttctg gtgggcagac aaaggcaaag gtaagaacga caacaaacct ctctctccgg
    33721 aaacctggca gcatctgaaa ggcctggtga ccaggcagct ttccggcaaa cgtctgttcg
    33781 ttgtcgacgc tttctgtggt gcgaacccgg atactcgtct ttccgtccgt ttcatcaccg
    33841 aagtggcctg gcaggcgcat tttgtcaaaa acatgtttat tcgcccgagc gatgaagagc
    33901 tggcaggttt caaaccagac tttatcgtta tgaacggcgc gaagtgcact aacccgcagt
    33961 ggaaagaaca gggtctgaac tccgaaaact tcgtggcgtt taacctgacc gagcgcatgc
    34021 agctgattgg cggcacctgg tacggcggcg aaatgaagaa agggatgttc tcgatgatga
    34081 actacctgct gccgctgaaa ggtatcgctt ctatgcactg ctccgccaac gttggtgaga
    34141 aaggcgatgt tgcggtgttc ttcggccttt ccggcaccgg taaaaccacc ctttccaccg
    34201 acccgaaacg tcgcctgatt ggtgatgacg agcacggctg ggacgatgac ggcgtgttta
    34261 acttcgaagg cggctgctac gcaaaaacta tcaagctgtc gaaagaagcg gaacctgaaa
    34321 tctacaacgc tatccgtcgc gatgcgctgc tggaaaacgt caccgtgcgt gaagatggca
    34381 ctatcgactt tgatgatggt tcaaaaaccg agaacacccg cgtttcttat ccgatctatc
    34441 acatcgataa cattgttaag ccggtttcca aagcgggcca cgcgactaag gttatcttcc
    34501 tgactgctga tgctttcggc gtgttgccgc cggtttctcg cctgactgcc gatcaaaccc
    34561 agtatcactt cctctctggc ttcaccgcca aactggccgg tactgagcgt ggcatcaccg
    34621 aaccgacgcc aaccttctcc gcttgcttcg gcgcggcgtt cctgtcgctg cacccgactc
    34681 agtacgcaga agtgctggtg aaacgtatgc aggcggcggg cgcgcaggct tatctggtta
    34741 acactggctg gaacggcact ggcaaacgta tctcgattaa agatacccgc gccattatcg
    34801 acgccatcct caacggttcg ctggataatg cagaaacctt cactctgccg atgtttaacc
    34861 tggcgatccc aaccgaactg ccgggcgtag acacgaagat tctcgatccg cgtaacacct
    34921 acgcttctcc ggaacagtgg caggaaaaag ccgaaaccct ggcgaaactg tttatcgaca
    34981 acttcgataa atacaccgac acccctgcgg gtgccgcgct ggtagcggct ggtccgaaac
    35041 tgtaatgatt tgaagctgga gaatatctat ccagtatctt atagaaagca aaacgggagg
    35101 caccttcgcc tcccgtttat ttacccttct tttgtcgtgc cctgcgcccg cgttaccggc
    35161 actggcagcc aggcgcgaat ggaaagcccg ccccgctcgc tggtgccaag ctccagcatc
    35221 ccgttatggt tatccacgat acgctgcaca attgccagcc ctaatcccgt gccgctaatg
    35281 gtgcgcgcac tgtcgccgcg gacaaacggc tggaacaggt gcttacgttg ttccggcgca
    35341 attcccggac cgtcatcttc cacctggaac caggcgcgat tcggctccgt tccgctgctg
    35401 actttgatcc agccattgcc ataacgggcg gcgttgacca ccatattcgc caccgcgcgt
    35461 ttgatcgaca gcgggtgcat tttcacgtca atgctgccgg ggtaaagcgc ggtttcaatt
    35521 tcccgctcat agccactttc ggcagcaatc acctcaccga gtactgcatt aagatccgcc
    35581 atttccatcg gcatctcctg cccggtgcgc aggtagtcga taaactgctc aatgatggcg
    35641 ttgcactctt cgatatcttt attgatcgat tctgccagat agccatcctg ctcgctcatc
    35701 atctcagtcg ccaggcgaat acgcgtcagc ggcgtgcgca agtcgtgact tacccccgcc
    35761 atcagcagcg tgcggtcatc cgccagttgc ttaacaccag ccgccatatg gttaaaggca
    35821 cgggtaacgg aacgcacctc ggacgcgcca tactcacgca gcggcggcgg aataatccct
    35881 ttaccaacct gcaaggctgc gtgttcgaga tcgaccaacg gtcggttctg gatacgaata
    35941 aacagccacg ccccgcctat cgccaatagc ataatcgcca gcgtatagcg gaacagcgga
    36001 gagaaatcgc cctgatgaat ttcggtcagc ggcacgcgta cccagatatt gggcgacagc
    36061 caggttttca gccagacgac aggcgaactt ttgttgacct caacgcgcac ttccgtcggg
    36121 ccgcccagtt gctgtgccat ctgatggctt aagaattcat agtgttgcgc ccaacgcaga
    36181 cctgcctctt cggcagcctc gttggagtag agagagatcc ccagctcacg gtagatctcc
    36241 cgacggaaag cgggaggcac aaccaactgc gtgccgtcct ccagttgcag tttgtcggtc
    36301 atcaacatac gcacttcgta cgcgaggact ttattaaact gctggaggct cggcaaaatc
    36361 gcgaagttca gcaccaccag ataagtcgtc accaggctgg cgaacagcaa ggtgacgatg
    36421 agcaataacg tacgggcaaa tgaacttcgt ggcgagaagc gcaatcgcct catgctttag
    36481 agccgtccgg tacaaagacg tagcccaggc cccaaacggt ctgaatgtaa cgcggatgcg
    36541 ctggatcttc ttctaccatg cggcgcagac gcgaaatctg cacgtcgatg gagcgttcca
    36601 ttgcggaata ttcacgacca cgggcaaggt tcatcagctt atcgcgggag agcggctcac
    36661 gcggatggct gaccagtgcc ttcagtaccg caaactcacc gctggtgagc ggcatcggct
    36721 catcttcgcg gaacatttcg cgcgtaccga ggttaagttt gaacttaccg aaagcaatta
    36781 ccgcctcttc ctgtgacggt gcgcctggca gttcgttcgc ctgacgacgc agcaccgcac
    36841 gaatacgggc cagcagttca cgcgggttaa acggttttgg aatgtagtcg tcagcgccaa
    36901 tctccaggcc tacgatacgg tccacttctt cccctttcgc cgtcaccata atgatcggca
    36961 tcgggttgct ctgactacga agacgtcggc aaatcgacaa gccatcttca ccaggtaaca
    37021 ttaaatccag taccataaga tggaaagatt cacgagtcag caggcgatcc atctgttctg
    37081 cattagcgac gcttcgaacc tggaagcctt gttcggtgag ataacgttcc agcagcgcac
    37141 gcaggcgcat gtcgtcatcg accaccagaa tcttgtagtt ctcttgcatt gtttgtactc
    37201 ccaaaggttc gcaacaattt gtaagcgtgt attcttaaaa aagctcacgt tcgtcaccag
    37261 ctaaatctgg tatgaatttc agcctaaatt gttacaaagc atattaaaca gcagcttaag
    37321 tatacaattt attcggcgaa acattattga ttctgttgat atgatcacgt tatacccaat
    37381 gtgcgcatta tcaaacagac aaagggaatc aacgagatga aaacgcccct ggttacccgg
    37441 gaagggtatg aaaaactcaa acaagagctt aattatctct ggcgtgaaga acgcccggag
    37501 gtcacaaaaa aggtgacctg ggccgcaagt ctgggcgacc gcagcgaaaa tgctgactat
    37561 cagtataata aaaagcgtct gcgtgaaatc gaccgtcgcg tgcgctatct cactaaatgc
    37621 atggaaaatc tcaaaatcgt cgattactcc cctcagcagg aaggcaaggt cttttttggc
    37681 gcatgggtgg agattgaaaa cgacgatggc gtgactcacc gtttccgtat tgtcggctac
    37741 gatgaaattt ttggccgtaa agattacatc tctatcgatt ccccgatggc ccgcgcattg
    37801 ctgaaaaaag aagtcggcga tctggcggtg gtgaataccc ctgccgggga agcgagctgg
    37861 tatgtcaatg ctatcgagta cgtgaaaccg taaggaagag tcttaacctc ctgccgatgg
    37921 ctggcatttt tgccagccag tccgtataac tatcccctga tttttgatcc gaaaagatga
    37981 actcaaacca tgatgaatga ttcgttctgc cgcattattg cgggtgaaat tcaggcgcgc
    38041 ccggaacagg ttgacgctgc cgttcgcctg cttgacgaag ggaataccgt gccgtttatc
    38101 gcacgttatc gtaaggaaat caccggcggt ctggatgaca cgcagctgcg taatctggaa
    38161 acgcgtctga gctatctgcg cgagctggaa gagagacgtc aggcgatcct caaatccatt
    38221 tccgagcaag gcaaactcac cgatgatctg gcgaaggcca tcaacgccac actaagcaaa
    38281 accgaactcg aagatctcta cctgccctac aaacctaaac gccgcacccg cgggcaaatc
    38341 gccattgaag cagggcttga gccgttggct gacctgctgt ggagcgatcc gtcacacacg
    38401 ccagaagtcg ccgctgcaca atatgttgat gcagataaag gcgtggcaga taccaaagcc
    38461 gcgctggacg gcgcgcgcta tatcctgatg gaacggtttg ccgaagatgc cgcgctgctg
    38521 gcgaaagtgc gtgattatct gtggaagaac gcgcatctgg tctcaaccgt gatgagcggt
    38581 aaagaagagg aaggggcaaa attccgcgac tatttcgatc atcacgaacc gctgtccacg
    38641 gtgccttctc accgcgcgct ggcgatgttc cgtgggcgta acgaaggcgt actccagctt
    38701 tcgctgaatg ccgatccgca gttcgaagag ccgccaaaag agagctattg cgagcaaatc
    38761 atcatggatc atctcggcct gcgcctgaac aatgctccgg cggatagctg gcgcaaaggc
    38821 gtggtgagct ggacctggcg catcaaggtg ctgatgcatc tggaaaccga actgatgggc
    38881 accgtgcgcg aacgcgcgga agatgaagca atcaacgtct ttgcccgtaa cctgcacgat
    38941 ctgctgatgg cggcccctgc cggactgcgt gccacgatgg gcctcgatcc gggtctgcgt
    39001 accggggtaa aagtagcggt ggtcgatgcc actggcaaac tggtggcgac cgataccatt
    39061 tacccgcaca ccggacaaac agcaaaagca gcgatgaccg ttgctgcctt gtgtgaaaag
    39121 cataacgttg aactggtggc gatcggcaac ggtacagctt cccgcgaaac cgaacgtttc
    39181 tatctcgacg tgcagaagca gttcccgaaa gtgaccgcgc agaaagtgat cgtcagcgaa
    39241 gctggcgcgt cggtttactc ggcttccgag ctggcagcgc aggagttccc ggatctcgac
    39301 gtttcgctgc gtggtgcggt atctatcgcc cgccgtttgc aggatccgct ggcggagctg
    39361 gtgaaaatcg atccgaaatc tatcggcgta ggtcagtatc agcatgacgt cagccagacg
    39421 caactggccc gcaaactgga tgcagtagta gaagactgcg taaacgccgt tggcgtcgat
    39481 ctcaacaccg cttctgttcc gctattaacc cgcgtggcgg gcctgacgcg catgatggcg
    39541 caaaacatcg tggcctggcg cgatgagaac ggccagttca agaaccgtca gcaactgctg
    39601 aaagtcagcc gtctggggcc gaaagccttc gagcagtgcg cgggcttcct gcgcattaac
    39661 cacggtgata acccgctgga cgcctctacc gttcacccgg aagcctatcc ggtggtggaa
    39721 cgcattctgg cagcaacaca gcaggcactg aaagatctga tgggtaacag cagcgaactg
    39781 cgtaacctga aagcgtctga ctttactgat gaaaaattcg gtgtgccgac agtaactgac
    39841 atcatcaaag agctggaaaa accgggtcgc gatccgcgtc cggaatttaa aaccgctcag
    39901 tttgccgatg gcgtcgagac aatgaacgac ctgcaaccgg gtatgatcct cgaaggcgct
    39961 gtcaccaacg tcaccaactt tggcgcgttt gtcgatattg gcgtgcatca ggacggcctg
    40021 gttcacatct cttcattgtc gaacaagttt gtggaagatc cgcataccgt ggtgaaagcg
    40081 ggcgacattg tgaaggtgaa agtgctggaa gtggatcttc agcgtaaacg tatcgccctg
    40141 accatgcgtc tggacgagca gcctggcgaa accaacgccc gtcgcggcgg cggtaatgaa
    40201 cgcccgcaaa acaaccgccc ggcagccaaa ccacgcggtc gtgaagcgca gcctgccggt
    40261 aatagcgcga tgatggatgc gctggcggcg gcaatgggca aaaaacgtta aacgccctga
    40321 ttgaccccta aacctgcgcg ataaggtgtc cacgccgcat ctggcaacga acgccttgtc
    40381 tgacataaaa gtgccggaga atatctccgg catttttatt ccacagccaa actcacaata
    40441 tattccggca acatttatca cttcattaac agctgaaacc ttaattaaac attagccagt
    40501 cctggtaatt cgctattcga attatatttt cgctgcgata taaccttgag ccacatcaac
    40561 attgagtcag attattattc aaaccaacat tcgcacacat tttaagtatt gctgatagaa
    40621 accattctca ttatcattgt gctgttgatt atttaatctc tccttcgttg gcaaatcatc
    40681 tggtctcatg tcgctgtcaa acgccccatg aggtagttat ccagttaatg agaaacaagt
    40741 aggcacctat gcaatacact ccagatactg cgtggaaaat cactggcttt tcccgtgaaa
    40801 tcagcccggc atatcgccaa aaactgcttt ctcttggcat gttacctggc tcctctttta
    40861 atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc
    40921 tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc tgttaatacg
    40981 gtgataacaa caatgaaaaa attaaccata ggcttaattg gtaatccaaa ttctggcaag
    41041 acaaccttat ttaaccagct cactggcgca cgtcagcgtg taggtaactg ggctggcgtt
    41101 accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac gctggtggac
    41161 ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc
    41221 gcctgtcact acattttgag tggcgacgcc gacctgctga ttaacgtggt ggatgcgtct
    41281 aaccttgagc gtaacctgta cctgacgcta caactgctgg aactcggcat tccctgcatc
    41341 gtggcactga acatgctcga cattgccgag aagcaaaata ttcgtattga aattgatgct
    41401 ctgtcggcgc gtcttggctg cccagtgatc ccgctggttt caacccgtgg tcgcggtatt
    41461 gaagcgctca agctggcgat tgatcgctat aaagctaacg agaatgtgga actggtgcat
    41521 tacgcacagc cgctactcaa cgaagcagat tcactggcaa aagtgatgcc ttccgacatc
    41581 ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat ctacagccgc
    41641 gcctacgccg gtgaagcgtc gcagcatctg gatgccgccc tcgcccgtct gcgtaatgag
    41701 atggacgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc tgccatctgt
    41761 gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc ggtagataaa
    41821 atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta cctgatgttc
    41881 ctgctggcta tcaacatcgg cggggcgtta cagccgctgt ttgacgtcgg ctccgtggcg
    41941 ctatttgtgc atggtattca atggattggc tacacgctcc acttcccgga ctggctgact
    42001 atcttcctcg cccagggcct gggtggcggc attaacaccg tgctgccact ggtgccgcag
    42061 attggcatga tgtacctgtt cctctccttc cttgaggact ccgggtatat ggcgcgtgcg
    42121 gcgtttgtga tggaccgtct gatgcaggcg ctgggcttgc cggggaaatc ctttgtgccg
    42181 ctgatcgtcg gtttcggttg taacgtaccg tcggtaatgg gtgcacgtac gcttgatgca
    42241 ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg
    42301 gctatcttcg cagtattcgc ggctgccttc ttcgggcaga acggtgcgct ggcggtcttc
    42361 tcgctgtata tgctgggtat tgtgatggcg gtgctgactg gcctgatgct caagtacacc
    42421 atcatgcgcg gtgaagcgac gccgtttgtc atggagttgc cggtctatca tgtaccacac
    42481 gttaaaagcc tgattatcca gacctggcag cgtctgaaag gcttcgttct gcgtgctggt
    42541 aaagtgatca tcatcgtcag cattttcctg agcgctttca acagcttctc gctgagcggg
    42601 aaaatcgtcg ataacatcaa cgactcggcg ctggcgtccg tcagccgggt gatcaccccg
    42661 gtcttcaagc caattggcgt gcatgaagat aactggcagg caacggttgg cctgtttaca
    42721 ggtgccatgg cgaaagaagt ggtagtgggt acgctcaaca ccctctacac cgcagaaaat
    42781 attcaggacg aagagttcaa tccggcagag tttaacctcg gtgaagagct gttcagtgcg
    42841 atagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact gatgaacccc
    42901 attgaagcca gcaaaggcga cggcgaaatg ggtaccgggg cgatgggcgt gatggatcag
    42961 aaattcggta gcgcagcagc agcttacagc tacctgattt tcgtcctgct gtatgtacca
    43021 tgtatctcgg tgatgggagc catcgcccgt gaatcaagcc gtggctggat gggcttctcc
    43081 atcctgtggg ggctgaatat cgcttactcg ctggcaacat tgttctatca ggtcgccagc
    43141 tacagtcagc acccaactta cagcctggtg tgcattctgg cggttatcct gtttaacatc
    43201 gtggttatcg gtctgctgcg ccgcgcgcgt agccgggtgg atatcgaact gctggcaacc
    43261 cgcaagtcgg taagcagttg ctgcgcagcc agcaccaccg gtgattgcca ttaatggctt
    43321 cacttattca ggtgcgcgat ttgctggcgt tacggggccg tatggaagcg gcccagataa
    43381 gccagacatt gaacactcca cagccaatga ttaacgccat gctgcaacaa ctggaaagta
    43441 tgggcaaagc cgtgcggatt caggaagaac ctgacggctg cctctctggc agttgtaaaa
    43501 gctgcccgga aggaaaagcc tgtctgcgcg agtggtgggc gctgcgttaa ccttactcca
    43561 tcgcctgttt tggaaagccc ggtatgcgtc tgcatccggg cttttttgcg tgcggctttc
    43621 cataaaaatg caactcttgc agcacggcgt aagttccttt gaaagcatct cgcagggatg
    43681 aaaactcgct aatacacagg tgtggagtgg cgcgtagagt cgcggcattc aaacaacagg
    43741 tgaaggaacg ccatgagcaa aaagcagagt tccaccccac acgatgcgct gttcaaactc
    43801 tttttacgcc aaccggacac ggctcgtgat tttcttgcgt ttcatttacc ggcacccatt
    43861 cacgcgcttt gtgatatgaa aaccctcaag ctggagtcga gcagctttat tgatgacgat
    43921 ctgcgtgaaa gctattccga tgtgctgtgg tcggtgaaaa cggaacaagg accaggatac
    43981 atctattgtc tgattgaaca tcaaagcacc tcaaacaaac tgatcgcatt tcgcatgatg
    44041 cgttacgcta ttgccgcaat gcaaaatcac cttgatgctg gatacaaaac gttgccgatg
    44101 gtggtgccat tgttgtttta ccacggtatt gaaagcccct atccctattc gctgtgttgg
    44161 ctggattgtt tcgccgatcc caaactggca aggcagcttt atgcctccgc atttccgctg
    44221 attgatgtca ccgtcatgcc tgatgatgaa atcatgcagc accgacgtat ggcgctgctg
    44281 gagttaattc aaaaacatat tcgtcaacgc gatctgatgg ggctggtaga gcaaatggcc
    44341 tgcttattaa gtagtggata cgctaatgac agacaaatca aagggctgtt taattacata
    44401 ctgcaaaccg gcgacgcggt acgttttaac gattttatcg acggcgttgc cgaacgttca
    44461 ccgaaacaca aggagagttt aatgacaatt gcggaaagat tgcggcagga gggggaacaa
    44521 tccaaagccc tgcatatagc caaaataatg cttgaatccg gagtccctct tacagacatc
    44581 atgcgcttta ccggggtgtc agaagaagag ttggctgcgg cgagccagta aatttctgtc
    44641 tcgccatttc aaaagttgcc tacaccctct gcttcaacgc caccagcagg tgacaaaact
    44701 cgaccggatg cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt
    44761 gaggccaaag tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc
    44821 catacaatcg caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt
    44881 tcaggatttc cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg
    44941 ttttcttcaa cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca
    45001 ggaaccgctc cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagta
    45061 cgtccggttt tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg
    45121 tgaccagcgc ctgaacacgc tcgggatggg ttaaaacaat ttggcttgcc accagcccgc
    45181 ccagactcca gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca
    45241 tatcagcaag tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa
    45301 ggtgcagcgt aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca
    45361 gtccccatcc gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga
    45421 tgttattcat ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac
    45481 cgggattatg ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg
    45541 tctgttcacg cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca
    45601 cacactccca tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg
    45661 tcacggttgc cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc
    45721 ggcgcagtga aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc
    45781 gtcgcaccac cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc
    45841 gtcactggcg tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt
    45901 tgcattgcca atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt
    45961 ttctcagtgc ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg
    46021 tgcaaggtcg ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag
    46081 agattgcgca gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc
    46141 gaaccttgta gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag
    46201 gccattacta tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca
    46261 aatcaggaag aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct
    46321 gaatgtggtg tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa
    46381 tttgacctgc tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag
    46441 atcgactttg ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa
    46501 atgcgtaaag tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg
    46561 cagatcaacc cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa
    46621 gacggttacg ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg
    46681 acgctgaaag aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg
    46741 cgcgatctca ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt
    46801 gtagattgcc cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga
    46861 cgttggcaca gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca
    46921 acctctcata atttaaattt accccgctct ggtgattctc aaacgccaga tgttacccgt
    46981 atcattcaca tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg
    47041 ggtatcagtc gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt
    47101 cagaaaattg acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt
    47161 gttgctatcg gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc
    47221 atcgctctcg tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa
    47281 gttattggct ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg
    47341 ggttttggcg caatgctggg caaaattctg gcagactgcg gtggcgcaca acgtatcgcc
    47401 accacgctga ttgccaaatt tggtaaaaaa cacatccagt gggcggtggt actgaccggt
    47461 tttaccgttg gttttgccct gttctatgaa gtgggctttg tgctgatgct gccgctggtg
    47521 tttaccatcg cggcttctgc gaatattcca ctgttgtatg ttggtgtacc aatggcagct
    47581 gcactgtctg tgacccacgg cttcctgcca ccgcatccgg gtccgactgc gattgccacc
    47641 attttcaatg ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc
    47701 gtgattctcg ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaattccg
    47761 gaaggtctct acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc
    47821 gtctggacct ctctggtgcc ggtagtactg atggcgatgc gtgcgattgc cgaaatgatc
    47881 ctgccgaaag gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggct
    47941 acgctgattg ccgtgctgat tgccatgttc acctttggtc tgaaccgtgg tcgttcaatg
    48001 gatcagatta acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc
    48061 atcggtggtg gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt
    48121 gcttccatga tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc
    48181 gtactgcgta tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg
    48241 gcaccgctga ttgcaacgac gggtgttagc ccggagctga tggttattgc ggtgggttcc
    48301 ggtagtgtga ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt
    48361 aacctgacta tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg
    48421 tgcggtctgg taggctgtct gctgctgaat atggtgattt gataacactg actgccggat
    48481 gcatcgcatc cggcatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg
    48541 tccaaatcct tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg
    48601 gaaagcttgc gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg
    48661 gccatatcca gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg
    48721 tagcgctgca aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg
    48781 gcacgttttg gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt
    48841 tcgcgatcct gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg
    48901 cccagcgtta gatccccgct ctcccagtaa ccgcgtagcg taggcaggtc atgtgtcgcc
    48961 gcaaccgcca tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg
    49021 ttttcgaaat agagcacttt gtaggagtac acaccgctgc tgcgcagctt accgacaatc
    49081 tctaccggta cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg
    49141 agtgccagaa tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc
    49201 gtctcgccat acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat
    49261 gcgccgcagt tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg
    49321 gtgatgatat gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata
    49381 tccggcggcg cgccaaccga cgctttcagg caatatagtt cacggtcaca ccaggtttcc
    49441 gccccacctt ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag
    49501 ccctggctta tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga
    49561 taaaaatcga cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg
    49621 ttctgataca tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc
    49681 tgggcatgta gcgcatcaaa ggctgcctgc cagaacaggc tgtcgccctg ctctgcaaca
    49741 aactggcgaa acgcggtcat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt
    49801 cgtaatgctg tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc
    49861 gcctgttgca gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta
    49921 agatggaaat cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa
    49981 gacgggctgt atgggctggc actctccgga tttgccggat agagcgcatg aatcgggttc
    50041 aggccgatga acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca
    50101 aaatccccaa taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc
    50161 cacagctttt gtttattcag caacgcctgc ggttcgtaac agcgtttcgg ggcgacaatc
    50221 acccggcaat gcgcgcgctg gtcgtcctgg gtgagtgtca gcgtgtgata accttccggc
    50281 agcttcgtcg gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgtgttcct
    50341 tcttcggtgg tcagcagcca gctatattcg ccgctgccct ccaccaccat cggcattttt
    50401 ttgccgctgg tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta
    50461 cgttgatgca tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta
    50521 ccgtgggcat tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt
    50581 ttgctttcca tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga
    50641 atagagcgat ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc
    50701 caggcctcct ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg
    50761 aaatccgcca tcaccagata cggatcgccg ccttgtttgc cgatactgtg cagcatctgg
    50821 tcgaaggcat gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg
    50881 tccagcacct tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc
    50941 ttcacttgtt ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg
    51001 atttcaacgt tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc
    51061 atattgccgg taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc
    51121 gccgggatca gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac
    51181 ttatcgccaa ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata
    51241 atattcttcg ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt
    51301 acgcgatcag cctgcgggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga
    51361 ttcaggtgct ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt
    51421 ggattgatct caataccggt acgaactttc acaaactccg ccagacggac tttattcgcc
    51481 tgcttgatct cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg
    51541 atcagctgat cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc
    51601 agtgcagggt tgcactgttt gatccagcga cgtggggtaa taccgttggt gacgttatgg
    51661 aatttgttcg gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag
    51721 tgcagcgccg caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc
    51781 acttgtttat cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt
    51841 ttctccacca gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc
    51901 agtaagcctt tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg
    51961 ttggtgtaag cgaaagtttt gctggtaatg gcccaggcgt catcccagct catctggtgc
    52021 tcatcgatca gcacgcgcag cagttctgga atcgcgatag ttgggtgggt atcgttcagc
    52081 tgaataactt cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc
    52141 aaaatatccg ctaccgaaca ggcacactgg aagtactgct gcatcaagcg cagcttttta
    52201 ccggcagtat ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgccc
    52261 tgctgttcgg cacgcaggaa atcaccgtcg ttaaatttag tcagatcaaa cggatgcgcg
    52321 tgcgtcgcct gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg
    52381 agatcccacg cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcgtcact
    52441 ttaccgccaa tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta
    52501 ctgcgatgcc agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac
    52561 aaaccatatt gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag
    52621 tcgaggaagc acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct
    52681 tcttccagca ggtccgtcag attgatgtca taagccttca acgaatcctg tacatcctga
    52741 taccagccga gattcaacag gttgttgccc gtcaggcgac caatcaaaaa ctccattgag
    52801 atgtagttaa catgtcgctg attcgccacc ggcttggcga atggctgagc acgcagcatt
    52861 tcggccagtg cttcactcac tgctagccac cactggcgag gagtcatttc agccgcagaa
    52921 tttaagccat aacgctgcca ctgacgtgaa agcgcttcct gaaattgctt atcgttaaaa
    52981 ataggttgtg acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt
    53041 ttgccaggct cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg
    53101 gggatgagca aggaaatgtg atctcaacca cttaaagcta gtgcaaacca caggattagc
    53161 agcaaatcaa tgcaatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc
    53221 gtattttcaa aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcat
    53281 aagtaaaata tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt
    53341 agatggcact ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat
    53401 aagggaaacc agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt
    53461 tggaattgtg acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa
    53521 aggtttctgg ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc
    53581 attttccaca gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg
    53641 actcgaccat accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt
    53701 ccggctggcg ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg
    53761 ggcggcaggc aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga
    53821 gcgtttcgcc agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat
    53881 atgtgagacg atggcgcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct
    53941 tttcattgag ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct
    54001 gatcactaat ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa
    54061 tctcaccctg gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt
    54121 tcgtgatcaa ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa
    54181 gcagtttttt gattgccgtc tgtcatcgcc gattgaagct gcagaaagca gtcggatttg
    54241 cgatgacgtt tccggttggg cgacggcact acagctaatc gccctctccg cccggcagaa
    54301 tacccactca gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc
    54361 ggattatctg gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt
    54421 gaaaagcgcc attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga
    54481 aaacgggcaa atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga
    54541 tgataccggc gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg
    54601 ccagtgggaa ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat
    54661 ggcccaggga tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat
    54721 gctgcgcgat attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct
    54781 gctggaagag tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt
    54841 gttattgcag gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct
    54901 agcccgtgct gaacatgaaa tcaaggacat cagagaaggc accatgcacg cagaatttaa
    54961 cgctctgcgc gcccaggtgg cgattaacga tggtaatccg gatgaagcgg aacggctggc
    55021 aaaactggca ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc
    55081 ggtgctgggt gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctaatgca
    55141 gcaaaccgaa cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat
    55201 ccagcaaagt gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacacagga
    55261 aaaagcgttc cagctgatca acgagcagca tctggaacag ctgccgatgc atgagtttct
    55321 ggtgcgcatc cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc
    55381 ggcgcgtagc gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct
    55441 ggcaatgttg attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagct
    55501 gaaccgtctg gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc
    55561 caacaaagtc cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg
    55621 gttgcgtcat acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg
    55681 caacattgcc cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga
    55741 agaactcaat gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt
    55801 gctgcttaat caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct
    55861 ggacgcatta aaactggcga atcgcaccgg atttatcagc cattttgtca tcgaaggcga
    55921 agcgatggcg caacaactgc gccagctgat tcaactcaat acgctgccgg aactggaaca
    55981 gcatcgcgca cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca
    56041 tttcgatgag aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactgatccg
    56101 caccagcccg ttgacccaac gtgaatggca ggtcctgggg ctgatttact ccggttacag
    56161 caatgaacaa attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg
    56221 caatctgtac caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa
    56281 actgctgaag atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt
    56341 ttacatcttt acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa
    56401 accgcaccgg caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat
    56461 gggcgacctt aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga
    56521 gatattcccc caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg
    56581 cggctaccac ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat
    56641 tttcactttc gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat
    56701 gaatattctg ttcatgcagg gaaaaactcc ccgccagtgt agcgatttca cgctcagcaa
    56761 catggcgcgg cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct
    56821 cgccaagttg caaggtgtta aacgatgcca cgggcgagac ttccgttgcc accacgccgc
    56881 ctccggcagg ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg
    56941 ccagcagcgg ctccagcacc cggcggataa aatccgcagg cggggccgac gggttatcgg
    57001 tgcctccgct cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg
    57061 tttgcagcac cagcgtacag cttccggcac taccgatagc aaagcggtac tcgccgccgc
    57121 gcacggtgcc aggccggaag accagacgct gcgaccccag ctccgcgcct tccaccgttg
    57181 ccctacaaat ttccgcagcc gcttttaccg cggtcagatg ctggcgcaac agccccggtt
    57241 tcgcccgccc ggcacgaatg ctggtgatgg taaatggctg gccggttatc atcgacaggc
    57301 tcagcgccga gcgcaggatc tgcccgccac cttcgccctg tgcgccatcc agcgcaatca
    57361 tccttttcat catttatcct tttacgcaca ccacctgacg cagggtatag ataacttcca
    57421 ccagatcgct ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt
    57481 cgatcacttc ggcatcttta cggcattcca catgcgcggt ggcacgaatt tgatcttcca
    57541 cgctgaacag ttttttcgct ttagtacggc tcattacccg cccggcaccg tggctgcacg
    57601 aacagaacga ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac
    57661 cgggaataat tccatattga ccagcacgcg cagacaccgc gcctttacgc gtcacgtaga
    57721 tctcttcacc aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca
    57781 tcgccagcgt ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca
    57841 cgttttccat catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc
    57901 ttaggtaatc atcaaagtat tccgtacctt ccataaagta cgccaggtcg cgcgatggca
    57961 acgtttcaag ctggtcctgc atctcttttt gtgccaaatc gataaagtaa gtcccgatgg
    58021 cgttaccaat tccgcgtgaa ccggagtgca gcataatcca cacctggtcc gactcatcaa
    58081 ggcagatttc aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag
    58141 tgttaaggaa acgcggatat ttttgcgtta accactgata accggcttca agctcagccc
    58201 atttcgcatc gacgttaaca ggcgggtttt cccatgcgcc tttatcacgt ttacaacggc
    58261 cagtggtacg tccgtgcggc accgcagttt caatcgcctg acgcagctct gccaggtttt
    58321 caggcaggtc tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca
    58381 cgcccaccgc cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac
    58441 ctttacccag gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg
    58501 ccgtattaat aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt
    58561 ttaccggggc attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt
    58621 gtttgctgtc gtgataattg caaccgtcgt gccagaaaat taaata
//
LOCUS       DAZFKM010000012        58602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFKM010000012 DAZFKM010000000
VERSION     DAZFKM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            318..4301
                     /gene="ehaA"
                     /locus_tag="ACHKAY_000853"
     CDS             318..4301
                     /gene="ehaA"
                     /locus_tag="ACHKAY_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO5699570.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            4443..5531
                     /gene="pdeL"
                     /locus_tag="ACHKAY_000854"
     CDS             4443..5531
                     /gene="pdeL"
                     /locus_tag="ACHKAY_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5699571.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(5573..6505)
                     /gene="yahB"
                     /locus_tag="ACHKAY_000855"
     CDS             complement(5573..6505)
                     /gene="yahB"
                     /locus_tag="ACHKAY_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5699572.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(6597..7094)
                     /gene="yahC"
                     /locus_tag="ACHKAY_000856"
     CDS             complement(6597..7094)
                     /gene="yahC"
                     /locus_tag="ACHKAY_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5699573.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            7352..7957
                     /gene="yahD"
                     /locus_tag="ACHKAY_000857"
     CDS             7352..7957
                     /gene="yahD"
                     /locus_tag="ACHKAY_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO5699574.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            7997..8860
                     /gene="yahE"
                     /locus_tag="ACHKAY_000858"
     CDS             7997..8860
                     /gene="yahE"
                     /locus_tag="ACHKAY_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5699575.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            8850..10397
                     /gene="fdrA"
                     /locus_tag="ACHKAY_000859"
     CDS             8850..10397
                     /gene="fdrA"
                     /locus_tag="ACHKAY_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5699576.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            10397..11815
                     /gene="yahG"
                     /locus_tag="ACHKAY_000860"
     CDS             10397..11815
                     /gene="yahG"
                     /locus_tag="ACHKAY_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5699577.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            11958..12908
                     /gene="yahI"
                     /locus_tag="ACHKAY_000861"
     CDS             11958..12908
                     /gene="yahI"
                     /locus_tag="ACHKAY_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO5699578.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            12918..14300
                     /gene="yahJ"
                     /locus_tag="ACHKAY_000862"
     CDS             12918..14300
                     /gene="yahJ"
                     /locus_tag="ACHKAY_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5699579.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            14599..15009
                     /locus_tag="ACHKAY_000863"
     CDS             14599..15009
                     /locus_tag="ACHKAY_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5699580.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            15263..16246
                     /locus_tag="ACHKAY_000864"
     CDS             15263..16246
                     /locus_tag="ACHKAY_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5699581.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            16295..17779
                     /locus_tag="ACHKAY_000865"
     CDS             16295..17779
                     /locus_tag="ACHKAY_000865"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5699582.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            17772..18743
                     /locus_tag="ACHKAY_000866"
     CDS             17772..18743
                     /locus_tag="ACHKAY_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5699583.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            18740..19696
                     /locus_tag="ACHKAY_000867"
     CDS             18740..19696
                     /locus_tag="ACHKAY_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5699584.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            19783..20832
                     /gene="yahK"
                     /locus_tag="ACHKAY_000868"
     CDS             19783..20832
                     /gene="yahK"
                     /locus_tag="ACHKAY_000868"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO5699585.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            21075..21890
                     /gene="yahL"
                     /locus_tag="ACHKAY_000869"
     CDS             21075..21890
                     /gene="yahL"
                     /locus_tag="ACHKAY_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO5699586.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            22302..22547
                     /gene="yahM"
                     /locus_tag="ACHKAY_000870"
     CDS             22302..22547
                     /gene="yahM"
                     /locus_tag="ACHKAY_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO5699587.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(22564..23235)
                     /gene="yahN"
                     /locus_tag="ACHKAY_000871"
     CDS             complement(22564..23235)
                     /gene="yahN"
                     /locus_tag="ACHKAY_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO5699588.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            23382..23657
                     /gene="yahO"
                     /locus_tag="ACHKAY_000872"
     CDS             23382..23657
                     /gene="yahO"
                     /locus_tag="ACHKAY_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO5699589.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(23755..25341)
                     /gene="prpR"
                     /locus_tag="ACHKAY_000873"
     CDS             complement(23755..25341)
                     /gene="prpR"
                     /locus_tag="ACHKAY_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO5699590.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            25580..26470
                     /gene="prpB"
                     /locus_tag="ACHKAY_000874"
     CDS             25580..26470
                     /gene="prpB"
                     /locus_tag="ACHKAY_000874"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO5699591.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            26515..27684
                     /gene="prpC"
                     /locus_tag="ACHKAY_000875"
     CDS             26515..27684
                     /gene="prpC"
                     /locus_tag="ACHKAY_000875"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO5699592.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            27718..29169
                     /gene="prpD"
                     /locus_tag="ACHKAY_000876"
     CDS             27718..29169
                     /gene="prpD"
                     /locus_tag="ACHKAY_000876"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO5699593.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            29209..31095
                     /gene="prpE"
                     /locus_tag="ACHKAY_000877"
     CDS             29209..31095
                     /gene="prpE"
                     /locus_tag="ACHKAY_000877"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO5699594.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            31434..32693
                     /gene="codB"
                     /locus_tag="ACHKAY_000878"
     CDS             31434..32693
                     /gene="codB"
                     /locus_tag="ACHKAY_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO5699595.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            32683..33966
                     /gene="codA"
                     /locus_tag="ACHKAY_000879"
     CDS             32683..33966
                     /gene="codA"
                     /locus_tag="ACHKAY_000879"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO5699596.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(34006..34905)
                     /gene="cynR"
                     /locus_tag="ACHKAY_000880"
     CDS             complement(34006..34905)
                     /gene="cynR"
                     /locus_tag="ACHKAY_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO5699597.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            35015..35674
                     /gene="cynT"
                     /locus_tag="ACHKAY_000881"
     CDS             35015..35674
                     /gene="cynT"
                     /locus_tag="ACHKAY_000881"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO5699598.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            35705..36175
                     /gene="cynS"
                     /locus_tag="ACHKAY_000882"
     CDS             35705..36175
                     /gene="cynS"
                     /locus_tag="ACHKAY_000882"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO5699599.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            36181..37362
                     /gene="cynX"
                     /locus_tag="ACHKAY_000883"
     CDS             36181..37362
                     /gene="cynX"
                     /locus_tag="ACHKAY_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO5699600.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(37441..38694)
                     /gene="lacY"
                     /locus_tag="ACHKAY_000884"
     CDS             complement(37441..38694)
                     /gene="lacY"
                     /locus_tag="ACHKAY_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO5699601.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(38746..41820)
                     /gene="lacZ"
                     /locus_tag="ACHKAY_000885"
     CDS             complement(38746..41820)
                     /gene="lacZ"
                     /locus_tag="ACHKAY_000885"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO5699602.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(41943..43025)
                     /gene="lacI"
                     /locus_tag="ACHKAY_000886"
     CDS             complement(41943..43025)
                     /gene="lacI"
                     /locus_tag="ACHKAY_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO5699603.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(43102..43935)
                     /gene="mhpR"
                     /locus_tag="ACHKAY_000887"
     CDS             complement(43102..43935)
                     /gene="mhpR"
                     /locus_tag="ACHKAY_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO5699604.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            44126..45790
                     /gene="mhpA"
                     /locus_tag="ACHKAY_000888"
     CDS             44126..45790
                     /gene="mhpA"
                     /locus_tag="ACHKAY_000888"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO5699605.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVTATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            45792..46736
                     /gene="mhpB"
                     /locus_tag="ACHKAY_000889"
     CDS             45792..46736
                     /gene="mhpB"
                     /locus_tag="ACHKAY_000889"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO5699606.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            46754..47620
                     /gene="mhpC"
                     /locus_tag="ACHKAY_000890"
     CDS             46754..47620
                     /gene="mhpC"
                     /locus_tag="ACHKAY_000890"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO5699607.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            47630..48439
                     /gene="mhpD"
                     /locus_tag="ACHKAY_000891"
     CDS             47630..48439
                     /gene="mhpD"
                     /locus_tag="ACHKAY_000891"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO5699608.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            48436..49386
                     /gene="mhpF"
                     /locus_tag="ACHKAY_000892"
     CDS             48436..49386
                     /gene="mhpF"
                     /locus_tag="ACHKAY_000892"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO5699609.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            49383..50396
                     /gene="mhpE"
                     /locus_tag="ACHKAY_000893"
     CDS             49383..50396
                     /gene="mhpE"
                     /locus_tag="ACHKAY_000893"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO5699610.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            50671..51882
                     /gene="mhpT"
                     /locus_tag="ACHKAY_000894"
     CDS             50671..51882
                     /gene="mhpT"
                     /locus_tag="ACHKAY_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO5699611.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            51984..52523
                     /gene="yaiL"
                     /locus_tag="ACHKAY_000895"
     CDS             51984..52523
                     /gene="yaiL"
                     /locus_tag="ACHKAY_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO5699612.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(52648..53481)
                     /gene="fghA"
                     /locus_tag="ACHKAY_000896"
     CDS             complement(52648..53481)
                     /gene="fghA"
                     /locus_tag="ACHKAY_000896"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5699613.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(53574..54683)
                     /gene="frmA"
                     /locus_tag="ACHKAY_000897"
     CDS             complement(53574..54683)
                     /gene="frmA"
                     /locus_tag="ACHKAY_000897"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO5699614.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(54718..54993)
                     /gene="frmR"
                     /locus_tag="ACHKAY_000898"
     CDS             complement(54718..54993)
                     /gene="frmR"
                     /locus_tag="ACHKAY_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO5699615.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(55179..55952)
                     /gene="yaiO"
                     /locus_tag="ACHKAY_000899"
     CDS             complement(55179..55952)
                     /gene="yaiO"
                     /locus_tag="ACHKAY_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO5699616.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            complement(55954..56535)
                     /locus_tag="ACHKAY_000900"
     CDS             complement(55954..56535)
                     /locus_tag="ACHKAY_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5699617.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            complement(56513..57709)
                     /gene="yaiP"
                     /locus_tag="ACHKAY_000901"
     CDS             complement(56513..57709)
                     /gene="yaiP"
                     /locus_tag="ACHKAY_000901"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO5699618.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            complement(57719..58390)
                     /locus_tag="ACHKAY_000902"
     CDS             complement(57719..58390)
                     /locus_tag="ACHKAY_000902"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO5699619.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
BASE COUNT        14152 a        14589 c        15906 g        13955 t
ORIGIN      
        1 tcggctgtat attaaatgta ttcctcttgc ttttgtttat attttgagtt gatttgagtt
       61 cctggataca tataatgcgc aggtattaga gactgggagg aatgtaatga taaagttaag
      121 agtgttaact taaaataagt tttaagtaat aacttgctat tttattaccc tctgaactac
      181 atactcagat atgtggttgc caatgctatg caaaattttt gaatttttat atgcgatgaa
      241 cagtaaccag aatggcattt aatgcgttgc tgtttatgca atcttggttt tatttggatg
      301 tattgctgga gattgtgatg aataaaatat atcggctaaa gtggaacagg tcccgtaact
      361 gttggagcgt ctgctctgag ctggggagca gagtaaaagg aaaaaagtcc cgggctgttt
      421 taattagcgc gataagttta tattcatctc tggtattcgc cgatgatgtc atcgtaaacc
      481 aggataaaac tattgatttt ggcaaagaga accagagcat cgattaccgt attacggtga
      541 ctgacaatgc caatctggta atcaatacga cagatacttc ccgtccgcgt ctgactctcg
      601 cttctggtgg tgggttggat attaccggag gaaaggtaac aatcaatggc ccgcttaact
      661 ttttgctgaa aggtacgggg ttcctgaatg tctccaatgc tggcagcgag ttatatgctg
      721 atgatttgta tgagtcaaac tcaggcatga gacatgatca aggctatttt aatgtctcca
      781 atggcggcaa aattcatgtt aagggcacca gtcgtctgac ttatacgcag gggaatgtaa
      841 gcggcgaagg tagtcaggta aatgcaggga ccttctttat gggcgtttac ggcggatatg
      901 gcggtaatca gttcctgtcg gttaataacg gtggtgaagt taatgctcgt gaacatatta
      961 gcctgggtta ttacgaccaa cgttctgata caacattagt cgtatcagat ggaggtaaaa
     1021 tatccgcacc gaaaattagt ttaagcacca actctgagtt agcgttaggt gcacaggaag
     1081 gaagtgctgc gaaggctgca gggattattg atgccgaaaa aattgagttt gtgtgggcaa
     1141 agacatccga taagaaaatc accttaaacc acacggataa aaacgcgact atttccgcgg
     1201 atattgtcag tggcagcgag ggcctgggct atatcaatgc gctcaatggc acgacttact
     1261 taaccggtga taactctgcc tttagtggta aagtcaaaat tgagcaaaat ggcgctttag
     1321 ggatcaccaa aaatataggt acagcagaga tcaacaaccg cgggaaatta cacctgaagg
     1381 ctgacgatag catgaccttt gccaataaga tttccggcaa tggtacaata agtatcgaca
     1441 gtgggacagt ggcactgacc ggcaataact atgcattcag cggatatatt gatgtcgctt
     1501 ctggtgctgt cgcggttatt tctgaagaca agaacatcgg ccgtgcggac ctggatgttg
     1561 atggcaaact gcaaatcaat gccaataaag attgggtatt tgataacgat cttcaaggta
     1621 gaggtattgt tgaaataaac atggggaatc acgaattctc cttcgatgag tttgcttata
     1681 cagactggtt ccagggttca ctggcgttcc agaacacgac atttaacctg gaaaagaatg
     1741 ctgagtttct gcagagaggc gggatcactg cgggtcaggg aagccaggta acagtgggta
     1801 agggcgctca ctccattagc actttgggat tctccggcgg aaccgttgat tttggtgccc
     1861 tgacagcagg tgcacagatg acagaaggga cggtcaacgt tagtaaaacg ctggatttgc
     1921 gcggcgaggg tgtgattcag gtttctgaca gtgacgttgt ccgctcagta tctcgtgata
     1981 ttgactctgc gttatcgctc actgaagtcg atgatggtaa cagcgccatt aagctggttg
     2041 atgcgcaagg tgcggaagtt ctgggcgatg cgggcaatct gcaattgcag gataaaaatg
     2101 ggcaaatcct ctccagcagc gcccaacgtg atattcagca gaatgggcaa aaagcggccg
     2161 tcggcactta cgactatcgt ctgacgagtg gggtaaacaa tgacggtctg tatatcggtt
     2221 acggcctgac ccagcttgat ttacacgcta ccgacagcga tgctctggtg ctgagctcta
     2281 acggtaaaag cgagaatgcc gccgatctca gcgcaaagat taccggcagt ggtgacctgg
     2341 cattcagcag ccagaagggt cagaccgtat cgctttctaa caaagacaac gactataccg
     2401 gtgttaccga tctgcgcagt gggacgcttt tgttgaataa cgataacgtg ttgggtaata
     2461 cccatgaact gcgtctggcg gcagagactg aactggatat gaatggtcac agccagaccg
     2521 tgggcacgct caatggcagc gccgattcac tgctgagctt aaatggcggc agtctgacgg
     2581 ttaccaacgg gggcacttca accggttcgt taacggggag cggagagctg aatattcagg
     2641 gcggcacgct ggacatcgcg ggcgataaca gcaacctgac ggcgaatgtg aacattgcta
     2701 attcggctaa tgtcctggta agtcatgcgc agggattggg tagcgcaaac gttgagaaca
     2761 acggtaccct ggcgttgaat aatagcactg aaaaaagagc ggctgcgtct gtgaattaca
     2821 ccctgggcgg caatctgacc aacaacggta cgctgatgac cggaatgtca ggacagcaag
     2881 ctggcaatgt gttagtggtg aaggggaact accacggtaa taacggtcaa ctagtaatga
     2941 atacggtact gaatggcgat gactcagtaa ccgataaatt ggttgtcgag ggcgatacta
     3001 gcggcacgac tgccgttacg gtgaataacg ctggcggtac aggtgcgaaa acccttaacg
     3061 gtatcgaact tatccatgta gacggtaagt ctgagggcga atttgttcag gctggtcgta
     3121 tcgttgcggg ggcgtatgac tacactctcg cgcgtggaca aggggcaaat agtggtaact
     3181 ggtatctgac cagcggcagt gattctcctg aactgcagcc ggagccagac ccgatgccga
     3241 atccggagcc aaacccgaat ccagagccga accctaaccc gacacctacg ccgggtccgg
     3301 atctgaatgt ggataatgac ctgcgaccgg aggcgggtag ctacattgcg aaccttgcag
     3361 cagcgaatac aatgttcacc acgcgtctgc atgagcgtct gggtaatacg tactataccg
     3421 acatggtgac gggtgagcag aaacaaacca ctatgtggat gcgccatgaa ggtggtcata
     3481 ataaatggcg tgatggcagc ggccagctga aaacccaaag caatcgctat gttctgcaac
     3541 tgggaggcga tgtcgcgcag tggagccaaa acggcagcga cagctggcat gttggggtca
     3601 tggcgggata tggcaacagc gacagcaaaa ccatttcctc gcgaaccggt tatcgtgcaa
     3661 aagcgagtgt gaacggatat agcaccggcc tctatgccac ctggtatgcc gatgacgagt
     3721 cgcgtaatgg cgcgtatctc gacagttggg cgcagtacag ctggtttgat aacacagtga
     3781 aaggggatga cttacaaagt gaatcctata aatcaaaagg atttaccgct tcactggaag
     3841 ctggatacaa acacaaatta gctgaattta atggcagcca gggaacgcgt aatgaatggt
     3901 atgttcagcc gcaagcacag gttacctgga tgggagtcaa agccgataag caccgcgaaa
     3961 gcaacggaac cctcgttcat agcaacggtg atggcaatgt tcaaacccga cttggcgtaa
     4021 aaacctggct gaagagccac cataaaatgg atgacggtaa atcccgcgag ttccagccgt
     4081 ttgtagaagt gaactggctg cataacagta aggatttcag caccagtatg gatggcgtgt
     4141 ccgtcactca ggatggagcc cgaaatattg ctgagataaa aaccggggtg gaaggacagc
     4201 taaatgccaa cctgaatgtc tgggggaatg tgggcgttca ggttgccgat aggggatata
     4261 atgacacctc tgcaatggtt ggcattaagt ggcaattctg atctgaataa tgagtaaaag
     4321 gagagagagc cgaacgggag ataattcggc tctctgtgtc attttcttgc agccagcttt
     4381 atcagtcaga gtgaagactc gcagatttag ctggcgcaca agatttaatc aaggtgtgtt
     4441 caatggcttc gcatgatttg agtgtttttt tggaagagtt cggggcaacg gtgaatttga
     4501 cgttgcctgg tatcgtgtcc gaaaaagaac ggcttttact caagctgcta atggagggaa
     4561 tgtcggttac agaaatctca cagtacagaa atcgcagtgc aaagacaatt tcacatcaaa
     4621 agaagcagct gtatgaaaaa ctaggcatcc agagtgatat taccttctgg cgggatatct
     4681 tttttcaata tcatccgcaa gtcatcagtg gcacgggaaa taaaaataat ttttatatcc
     4741 ctgataaccg ttgtcaccat atcgtcacgc ctgaagccat cagtctggcg ttggaaaacc
     4801 acgaattcaa accgtggatc caaccggttt tctgcgcgca gactggggta ctgacgggct
     4861 gtgaggtact tgtccgctgg gaacatccac aaacgggaat tatcccaccg gatcagttta
     4921 ttcctctggc ggagtcatcc ggtcttattg tcataatgac ccgccaactg atgaaacaga
     4981 ctgcggatat tctgatgccg gtaaaacatt tgctgccgga caatttccat attggcatca
     5041 acgtctcggc gggttgtttt ttggcagcgg gatttgaaaa agagtgtctg aacctggtta
     5101 agaaattagg taacgataaa atcaagctgg ttctcgagct aacggaacgt aaccctattc
     5161 cggtaacgcc agaagccaga gcgatatttg acagccttca tcagcacaac attacctttg
     5221 cgctggatga ctttggtacg ggttatgcga cctatcgtta cttgcaggcg ttcccggtcg
     5281 attttattaa gatcgataag tcatttgtgc aaatggcgag tgtcgacgaa atctccggtc
     5341 atattgtgga caatattgtc gaactagcgc gtaagcctgg tctgagtatc gtggcggaag
     5401 gggtagaaac ccaggagcag gcggatttaa tgatcggtaa aggcgttcac tttttgcagg
     5461 gctatttgta ctctccgcca gtaccgggta ataaatttat ctctgaatgg gtaatgaaag
     5521 caggtggttg atgtaaaccg ctattcacag cgcatcggga ggttggcagc gattaattct
     5581 cccgatgcag taaatcctga tagatccccg ttaatatgcc atttggcgca aattgttttt
     5641 taatccactg cgtcacctga cccgtagcag aatgctgagt cgccagtaac atgcgcgaat
     5701 cctggcgcgg attatggatt tgtcgggtaa ccagcaggga ttgcgtcatc gcctcacgga
     5761 ccatgtaatc cggcaaaaaa ccaattcctt cacctaaaat ctggcactga catttggtgt
     5821 tgaaatctgg caccagaatt gactcctgcc cgtgcagcaa ccagcccact tttttattaa
     5881 tcgtatgcgc ggtgtcctcc accataatat tagggtagag acgcagttgg ctttcggcga
     5941 tgggttccgg cacaaaggct aacgggtgat ccggggcgat agcaaaagcc cagcgaatcg
     6001 cgccaatttc agtgtaatca ataccgccgc cgtccagtag tgtatcgggt gcgccgatgg
     6061 cgatattggc ctgattatta ataatcgcat cccagacgcc gttatacact tcggtggtga
     6121 cggtaatctg gcaggtagga aactgctttt tcagcacctg taacaagcgt gcggtgtgtt
     6181 tgggcgtata aagcagctga ttaatacaaa tgcgcacacg cgcttctatg ccctgcgaaa
     6241 tagtatcgat actgcgtttg atggcataaa aatcattcag caggtcggta gctttgcgga
     6301 aaaaataacg cccggactcc gttaactcaa tgctgcgggt actgcgagtg aacagcacca
     6361 catccagccc cgtctccata cgcttgatgg tgtagctaat ggcggaagtg gttaaaccca
     6421 actcttctgc ggctttactg aagctgccaa aacgcgcagc ggtagtaaat gccaacagat
     6481 tctcttcggt aaaaattgag ttcattaaac cattctcgcc attatctact atgaatttct
     6541 ccagctatga ctgtagaggt atcggttaaa gatagataat catttttgaa taacttttaa
     6601 tacccgtcgc gtttagtcgt cgcttcgctt aatttttgtc caccatattc tgaaattatc
     6661 cccacgcagt tacccgccag aaaacccagt aacgtcaccg tccagttcat cccgctggca
     6721 aaaaacagcg tcatgccgag aaagccaccg ggaataaagg acagtaacca gaaacgccct
     6781 tgccagacca ctaccgccga gaagggtacg gtagtaatta cgctcgccca gaacgcggac
     6841 agtccactta cagaggccag ccaaccggca gccagcgcgg cgaaaaatgc ccacaccatc
     6901 ccggaataat taaccgccag acttctgata aaccccgatt taccgccgcc tgcggcataa
     6961 aaactgcaaa aggcgacgaa gccaatggtt cctaataatt cccatccctg agagagcccg
     7021 acatgtgaag acactaactg ccaaagccct gcacaaatac cgacggtgac acccgttgcc
     7081 gtgagtccgt tcatcttgtt cccctctggt tagacatgga cgaaagctac tcttacatgt
     7141 gcagactata gagaaataaa acctgattga gtgaaagggt atcgggtcaa agaaacaaat
     7201 attgcactac cgcacactgc gaaaagattg ttgaacatca ttcaacaaaa aggcgaaata
     7261 cagcgaatta aatttaagcg ggttctttta tcagattatc gttgctattc tcaattcatc
     7321 gaaaacagcc acaaaaacca ggggaatgtg aatgagtata aaaaatctac ctgccgacta
     7381 tttattggct gcacaacagg gtgatatcga taaagtaaaa acctgccttg cgctgggtgt
     7441 cgatattaat acctgcgatc gtcaggggaa aacggcaatt acgctggcaa gtttatatca
     7501 gcaatatgct tgcgttcagg cattaattga tgccggagcg gatattaata agcaagatca
     7561 tacctgttta aatccttttt taattagctg tctgaacgat gatttaacgc tactacgaat
     7621 tattttaccg gctaaacccg atcttaattg cgtaacccgt tttggcggtg tcggcctgac
     7681 gcctgcctgt gaaaaaggcc atttaagtat tgtaaaagag cttttggcgc atacggagat
     7741 taacgttaac cagaccaacc atgtaggctg gacgccgcta ctggaagcga ttgtgcttaa
     7801 tgatggtggt attaaacagc aggcgattgt gcagttattg ctggaacacg gtgccagccc
     7861 gcatctgacc gataaatatg gcaaaacgcc actggaactg gcgcgggaac agggctttga
     7921 agagattgcg cagttactga ttgccgcagg tgcataaacc gggaggcttg ctatcaacac
     7981 accagaaaga cggtgtgtgt gggcgctaac tgcggatgcg gattttctgg cgcagcgggg
     8041 gcaaggacag gttgaacagg tctttgccag agcggtaaat atcgcactcc cggctcgcca
     8101 gcagttgctg acgctgcttt gtgaagagta cgacaatgcg ccaaacagtt gtcggttggc
     8161 actcactcac tttgatgatc tgttccggca tggtgataag gttcagtttg acgatcaagg
     8221 tattacggtt ggtcaacatc ttcatataga gatgagtcgt tgtcggcgtt ggctgtcccc
     8281 aaccttgcaa atgaccgctg tgaattttca ccttatcgcc tggctacagt ggcacgacat
     8341 tattcatcag cacctggggg aaaatgaaac cctgtttaat tatcgcggcg ataatccgtt
     8401 ttatcaggcg ttaaataaag aactacatat taaacgacgg gcagttattc aggcagtaaa
     8461 cgaaaaacaa aatatcgccg cagcggtcgc cagtatgatt gggttaggga ttggccttac
     8521 gccatcagcc gacgattatt taacaggtct ggcgcttatt ttatttattc ccgggcatcc
     8581 gtcggaaaaa tacaaagagg aattttatct cggtatgcag cgcggcagaa ataacaccac
     8641 attattaagt gctataacgc tggaagccgc attacaacaa cgttgccggg aaaatattca
     8701 tcgttttatt cacaacatta tttatgacat ccctgggaac gcaactcagg caatagaaaa
     8761 aattaaacat attggctcca gttccggctg cgacatgctg tatggcatgg ccgatggttg
     8821 tgcgctgagc caaacctacg gagggaatta tgtcagttaa aatagtcatt aaaccgaata
     8881 cctattttga ttctgtctcg ctgatgtcta tctccacgcg tgcaaataaa ctcgacggcg
     8941 tcgagcaggc atttgtggcg atggcgaccg aaatgaacaa aggtgtactg aaaaatttag
     9001 gactgctgac gccggagctg gaacaggcga aaaacggcga cctgatgatt gtcatcaatg
     9061 gtaaatcggg tgcggacaac gagcagttgc tggtggagat tgaagaactg ttcaacacca
     9121 aagcgcaaag cggctcgcac gaggcgcgtt acgccactat tgccagcgcc aaaaagcata
     9181 tcccggaaag taacctggcg gtgatttcgg tcaacggtct gtttgccgct cgcgaagcgc
     9241 gtcaggcgct gcaaaacgat ctcaacgtga tgctgttttc cgataacgtc tcagttgaag
     9301 atgaactggc gctcaagcag ttggcccacg aaaaagggct gctgatgatg gggccggact
     9361 gcggcacggc gattattaac ggcgcggcgc tctgttttgg taacgccgtg cgtcgcggca
     9421 atatcggtat tgttggcgca tccggcaccg gcagccagga gctgagcgtc cgcattcatg
     9481 aatttggtgg cggtgtttca caactgattg gcaccggcgg gcgcgacctg agcgagaaaa
     9541 tcggcggcct gatgatgctc gacgccatcg ggatgctgga aaacgatccg caaactgaaa
     9601 ttatcgcact gatttcaaaa cctcccgcgc ctgcggtggc ccgcaaagtg ctggaacgcg
     9661 cacgtgcctg ccgcaagccg gtggtggtct gcttcctcgg tcgcgttgaa acgccggtgg
     9721 atgagcaggg gctacagttt gcccgcggca gcaaagaggc ggcgctaaaa gcggtgatgc
     9781 tctccggcgt gaaacaggaa aatctcgacc tgcatacgct taaccagccg ttgattgctg
     9841 atgtgcgtgc gcgtctgcaa ccgcagcaga aatacattcg tggcctgttc tgcggcggca
     9901 cgctgtgcga cgaaaccatg ttcgcggtga tggaaaaaca tggcgatgtc tacagcaaca
     9961 ttcagcccga tccggaattc cgcctgcaag atatcaaccg cagcatcaaa cacaccttcc
    10021 tcgactttgg cgatgacgac ttcacgaacg gcaagccgca tccaatgatt gaccccacca
    10081 accgcatcag tcgcttgatc gaagaggcgc gcgatccaga agtggcggtg atcgtgatgg
    10141 attttgtgct cggatttgga tcgcatgaag atccggtcgg ctccaccatc gaggcgatca
    10201 aagaagcgaa agcgatcgcc gctgccgaag gacgcgagtt gatcattctc gcctatgtgc
    10261 tgggtaccga tcttgatacg ccatcgttag aacaacaaag ccagatgctg cttgatgccg
    10321 gagtgattct ggcgagcagc agcaccaata ccggattgct ggcgcgtgaa tttatctgca
    10381 aaggggagga agcctgatga gccagtcact gtttagccaa ccattgaacg ttattaacgt
    10441 cggcatcgcc atgtttagcg atgacctgaa aaagcagcat gtagaagtga ctcaactcga
    10501 ctggacgccg ccggggcagg gcaatatgca ggtggtgcag gcgctggata acattgccga
    10561 ttcgccgctg gcagacaaaa tcgccgccgc taaccagcag gcgctggagc gcattatcca
    10621 gtcgcatccg gtgctgattg gttttgatca ggcgattaac gtggtgccgg gcatgacgcc
    10681 gaaaaccatt cttcacgccg ggccgccgat cacctgggaa aaaatgtgcg gcgcgatgaa
    10741 aggcgcggtc accggggcgt tggtgttcga agggctggcg aaagatctcg acgaggcggc
    10801 tgaactggcg gcctccgggg agatcacctt ctcgccgtgc cacgaacacg actgtgtggg
    10861 atcgatggcg ggtgttacct ctgcctcgat gtttatgcac atcgtaaaaa acaaaaccta
    10921 cggcaacatc gcttacacca atatgagcga gcagatggcg aagattttgc gtatgggagc
    10981 caacgatcag agcgtgatcg accgcctcaa ctggatgcgc gatgtgcagg ggccaatgct
    11041 gcgcgacgcg atgaaaatta tcggcgagat tgatctgcgc ttaatgctgg cgcaggcgct
    11101 gcatatgggc gatgagtgcc ataaccgcaa taacgccggg acgacgttgc tgattcaggc
    11161 gctgacgccg ggaattattc aggcaggtta ttccgtggag aaacagcgcg aagtgtttga
    11221 gtttgtcgct agcagcgact acttctccgg cccgacgtgg atggcgatgt gtaaagcggc
    11281 gatggatgcg gcgcacggca tcgaatacag caccgtggtc accaccatgg cgcgtaacgg
    11341 cgtcgagttc ggcctgcggg tcagcggatt accggggcaa tggtttaccg gtccggcgca
    11401 gcaggtcatc ggcccgatgt ttgccggtta taagccggaa gattcggggc tggatatcgg
    11461 cgacagcgcc atcactgaaa cctacggtat tggcggattt gctatggcga ccgcgcctgc
    11521 aatcgttgcg ctggtgggcg gcacggtgga agaggctatc gatttctctc gccagatgcg
    11581 tgaaatcact ctcggtgaaa accccaacgt caccattccg ctgctcggtt ttatgggcgt
    11641 gccgtcggca atcgacatca cccgcgtggg tagcagcggc attctgccgg tgatcaacac
    11701 tgccatcgcc cataaagatg cgggcgtcgg catgattggc gcgggcattg tgcatccacc
    11761 ttttgcctgc ttcgagaaag ccattcttgg ctggtgcgaa cgttacggcg tctgacctac
    11821 atgttcatgc cggatgcggc gtgaacgcct tatccggcct acggatggcc cgagaattgt
    11881 aggcctgata agcgtagcgc atcaggcagt ctggcgttgg tcataaccta atcaccctct
    11941 gtagcggaca taacaacatg aaagagcttg tggtcgttgc cattggtggc aacagcatta
    12001 tcaaagataa cgccagccag tcgattgagc atcaggcgga ggcggtgaaa gccgtggccg
    12061 atacggtgtt ggaaatgctg gcttccgatt acgacattgt gctgacccac ggtaacgggc
    12121 cgcaggtcgg gctggattta cgccgtgcgg agattgccca cgagcgcgaa gggctgccct
    12181 taacgccgct ggcgaactgt gtggcagata cgcagggcgg catcggctat ctgatccaac
    12241 aggcgctgaa taaccggctg gcgcgtcacg gcgagaaaaa agccgtcacc gtggtaactc
    12301 aggtggaagt ggataaaaac gatccaggtt ttgcccatcc caccaagccc attggtgcat
    12361 tcttcagtga aagccagcgt gacaaactac aaaaggcaaa ccctgactgg tgttttgttg
    12421 aagatgccgg acggggctat cgccgcgtgg tcgcctcgcc ggaaccgaaa cgtattgtcg
    12481 aagcacccgc cattaaggcg ctgatccaac aaggttttgt cgtaattggc gcgggcggcg
    12541 gtggaattcc ggtagtgcgt actgaagcgg gggattacca aagcgtggac gcggttattg
    12601 acaaagatct ctctaccgcg ctgctggccc gtgaaattca cgccgacatt ctggtgatca
    12661 ccaccggcgt cgaaaaagtc tgtatccact ttggtaaacc gcagcagcag gcgctggatc
    12721 gggtggatat tgccaccatg actcgctata tgcaggaagg gcattttccg cccggcagca
    12781 tgttgccaaa aatcatcgcc agcctgacgt tcctggagca gggcggcaaa gaagtgatta
    12841 tcaccacgcc ggaatgcctg cctgcggcgc tgcgcggtga aacgggcacc catattatta
    12901 aaacgtaagg acgtaggatg aaagaaagca atagccgccg tgaatttctg agccagagcg
    12961 gtaagatggt caccgccgcc gcgctgtttg gtacctctgt gccgctcgcc catgcggcgg
    13021 tagctggcac cctaaactgc gaagcgaaca acaccatgaa aatcactgac ccgcattact
    13081 atctcgataa cgtgctgctg gaaaccggtt ttgactacga aaacggtgtg gcggtgcaga
    13141 cccgcacggc gcgccagacc gtggagattc aggacggcaa aattgtcgcc ctgcgcgaga
    13201 acaagcagca tccggacgcc acgctgccgc actatgacgc tggcggtaag ttgatgctac
    13261 ccaccactcg cgacatgcat attcatctcg acaaaacctt ctacggcggg ccgtggcgct
    13321 cgctcaatcg tccggcaggc accactatcc aggacatgat caagcttgag cagaaaatgc
    13381 tgccggaact gcaaccgtac acgcaggaac gggcagaaaa actgatcgat ctattgcagt
    13441 cgaaaggcac caccattgcc cgcagccact gcaatatcga accggtttcc ggcctgaaaa
    13501 atctgcagaa tttgcaggcg gtgctggcgc gacgtcaggc gggcttcgag tgtgaaatcg
    13561 tcgccttccc gcagcacggt ttgctgctgt cgaaatcgga acccttaatg cgcgaagcga
    13621 tgcaggcggg tgcgcattac gtcggcgggc tggacccgac cagtgtcgat ggcgcgatgg
    13681 aaaaatccct cgacaccatg tttcagattg cgctggacta cgacaaaggt gtcgatattc
    13741 acctgcacga aaccactccg tcgggcgtgg cagccatcaa ttatatggtt gaaacggtag
    13801 agaaaacgcc gcaactgaaa ggtaagctga ccatcagcca cgcctttgcg ctggctacgc
    13861 tcaacgaaca acaggtcgat gcgctggcga atcggatggc ggcgcagcaa atttctatcg
    13921 cctcgacggt gccgattggc acgctgcata tgccgctcaa acagttgcac gacaaaggcg
    13981 taaaagtgat gactggcact gacagcgtta tcgaccactg gtcgccttat ggtctgggcg
    14041 acatgctgga aaaagccaat ctctacgcgc agctctatat tcgtcctaac gaacagaacc
    14101 tctcccgttc gctgtttcta gccactggcg atgtattgcc gctgaatgaa aaaggcgagc
    14161 gtgtatggcc aaaagcgcag gatgatgcca gctttgtgct ggtggacgcc tcctgttccg
    14221 ccgaggcggt ggcgcgtatc tcgccgagaa ccgcaacgtt ccataaaggg caactggtgt
    14281 gggggagtgt ggcaggttga tgcgcatttg aaacgcctgc aacggtgaat tgtaagagat
    14341 ttaagcccca gggagtgggg cgggaatggt ttgctaatga atgatcctga actttataat
    14401 ctcgcggcat aatttccagg ggattatttg agagaaatac acaaaagtga gttttaacaa
    14461 aggtatttat caggccataa atgtgacctg cttcggataa aaaagattca gtatcgaaaa
    14521 accaatgata ctcttgctga tggttaccaa cagtaattaa gatgtgattt gtttttccgt
    14581 aatcgcaaaa atattgccat gaccataaaa gaaaaattct cacaaaaata tccccatgct
    14641 tccttttgta cttttggcga ttcagcagcg ttggccgatc acctcgcaac gttgattgcg
    14701 actggtgtta aaacagcttc ctgtggttcg ctggcgggat gtattgaaga caacgcattt
    14761 ccgatgattg gtgagtataa gattgtagaa aatagccgtg gtgagcctgt atgtgttatc
    14821 agagtgattg gcctgcattt actgcgcttt tctgatgtca ccgtggagct tgcccgtaag
    14881 gaaggtgaag gtgacctcag tcttgaatat tggcgtaatg aacaccgtcg ttttttccag
    14941 gcagaaggaa gctattcacc agaaatggac gttatatttg aagaatatgc ccttattgac
    15001 gttgtgtaga taactattta tctacgcgaa ataaactcac acacataaaa tatcgctaat
    15061 tttgtttatt ttgctatttt ttacgtgatg aatgtcacac ttattgttgc tgcccgtttt
    15121 gtccatattt ataaccatat ttttaatagc tgcctgaaat agggctgttt attctgcata
    15181 aaaatattcg aaaattctta ttctctccag cgcgctaatt tgggcgaaca cttcctgact
    15241 accctgcaat gaggctgaaa tgatgaataa acgttttgtt atcaatatgg tgtcttcgct
    15301 gttattaggc gctgcgctaa tttctgcgcc gctccaggca gcggaaaaag tcgtggtgaa
    15361 tatctctaaa gtcgatggta tgccgtggtt taatcgcatg ggcgaaggtg tggttgaggc
    15421 gggtaacgcc tttggtgtta atgcgtcaca ggtggggcct tcaagtactg atgctccgca
    15481 acaagttaaa attattgagg atttaatcgc ccgtaaggtt aatgcaatta ctatcgtgcc
    15541 aaacgatgcc aacgtgctgg aaccggtatt caaaaaagcg cgagatgccg gaattgtggt
    15601 tctgaccaac gaatcccccg gtcagccaag tgctaactgg gatatcgaaa tcattgataa
    15661 cgaaaaattt gccgctgaat atgttgagca tatggcgaaa cgcatggggg gcaagggcgg
    15721 ctatgtgatt tacgtgggta gtttgaccgt gccgcagcat aacttatggg ctgacttgct
    15781 ggtgaaatac cagaaagagc attacccgga tatgcatgaa gttacccgcc gcatgccggt
    15841 tgccgagagt gtggatgact cacgtcgtac cacactggac ctgatgaaaa cctatcctga
    15901 cttgaaagcg gttgtctcct ttggttcaaa cggtccgatt ggcgcgggac gtgcggtgaa
    15961 agagaaacgg gcgaaaaata aagtcgcagt ctatggcatg atgatccctt cacaggcggc
    16021 atcactgatt aaaagtggtg atatcaccga agggattact tacgattcgg cctctgcagg
    16081 ttacgcactc gcagccgtcg ccagcacgtt gctgaagggt gaagagatca aacctggatt
    16141 agaaatgcag aatctgggta aggccgacgt agatatggat aagcgaatta tccgtttcca
    16201 taaggtatta ctggtcaaca aagacaatat cgactcactt tattaatcca tttcttcggg
    16261 gcgaaagccc cgattcttat tgccagggga tgttatggaa accttccttt cccttcgtca
    16321 tatcaacaaa acgtttcatg ccacgcgagc gctgcgcgat gtttctctcg actttatgtc
    16381 gggtgaagtg cactgccttg ccgggcaaaa cggctgtggt aaatcgacgt tgattaaaat
    16441 tatgtccggt gtttatcgcc cggatgaggg agcggaaatt acgcttggtg ggaaaaactg
    16501 gtcaaagctg acacccgccg cttcggtggc gcaggggatt caggtgattt atcaggacct
    16561 ctctttattt cctaacctga gtgtctggga gaatatcgcc gtgaatcact atcaccacgg
    16621 tctgtttgtt aaccgccgtc gtctgcgtga ggtggcgcag gcggcaatga ccagtattaa
    16681 cgtcacattg ccactggata cgctcgtttc tgagctttca attgcccgct gtcagctggt
    16741 agcaatttgc cgtgcactgg cgcaggacgc acggttgatc gtgatggatg aaccaaccgc
    16801 ttcgctgacc catcaggaag tgcaagggtt attacaggtg gtgcatcaat tgcgggaacg
    16861 cgggatctgc gtggtcttcg tcagtcatcg tctggaagaa gtgatggaag tttccgaccg
    16921 tatttcagtg ctgaaagatg gtgagctggt cgggactttt ccggctgcag agatgaccac
    16981 aaaacaactc ggtttcctga tgaccggcca ggagttcgaa tatcaggtgc gagagttgtg
    17041 gcagggaaaa tccagcaccc cggtgcttga ggtgcgtaat ttaagtcgtc atggggaata
    17101 tctaaatatt aacttgcggg tggaagcggg cgaagtggta tcgattgttg gcctgcttgg
    17161 tgcggggcgc acggaattat gtctgagtct gtttggtatg acccgaccgg atgctggcga
    17221 gatcctcatc aacggccagc cggttacact gcatagtaac caggacgcta tccgccacgg
    17281 tattggttat gtttcggaag atcgcatgtc gcgcggtttg gtgatggcgc aatctattga
    17341 agacaacatc attagcactg tttttcacaa ggtaaaagat cgcttcggct ttctgagtga
    17401 aactaaagtt cgtgacctgg tggacaggct aatcaaggca ttgaccatca aagtctctga
    17461 tcctcatctt ccggtgaata cgctttctgg tggtaacgcg cagcgagtat cgatcgccaa
    17521 atggctggcg attgggccac gactgttgat tctcgatagc ccgacagtcg gtgttgatat
    17581 cgccaacaaa gcaggaatat acggcattat cagcgatctg gcggctcatg gcattgcggt
    17641 attgatgatc tgcgatgaaa ttgaagaagc gtggtatcaa agccaccgga ttttggtaat
    17701 gcaaaaaggt cagatcaccc atagcttcct gcctgacagc agttctcagg ccaggattgc
    17761 ggaggtggta aatggctgaa ctgaaaaaac gccatgaatt ctggctggca ttactgattg
    17821 tggtgctgtt tgttggcctt gcctggcgca gcgacgagtt tctgacattc ggtaatttgt
    17881 acgatctcgc caataactat gccatgttga ctattctcgc ctgtggtttg tttgtggtgt
    17941 tgatttccgg tggaattgat atttcgtttc cagcaatgac catcattgcg caatacggca
    18001 tggtgctgtt gctgcaaaaa attggtggca acttcgctgt cgcgtttgca ctggcgggct
    18061 gcatcggcat tttacttggc ttaattaacg ccttactggt taatcgccta cgggtgcctt
    18121 ctatcatcat cactatctcg acgctgaata ttttctatgg cctgctgtta tggttgagta
    18181 aaggtgtgtg gctgtacgac tttccgccgt ggtttgagca gggggttatg ttgttcaagt
    18241 acaccgatgc tgatggctat gactatggcc ttggtctgcc gctgatcgcc atgattacgg
    18301 tggtgctgct gacagcgttt atcatgaatt tcaccagtgt agggcgcaaa atttatgccc
    18361 ttggcgggaa ccgcgaatca gccagtcgca tcggctttag cgtgctgaaa ctgcaacttt
    18421 tcgtctacgg ctatatggga ttgatgtctg gcgctgcggg tgtagtgcag tcgtggacgg
    18481 tgatgactgt cgcccccgat tctcttctgg gttatgagct gacagtactg gctgcggtgg
    18541 tgcttggcgg cactagtttg ctcggcgggc gcggcacgtt aaccggtact ttgctcggcg
    18601 tggtgttgtt ggcagtgatg caaaacgggc taaatttatt gggagtctcg tcttactggc
    18661 aaacattgat caccggcatc atcatcgttg ccagcattag tgccacggcg tggagtcagc
    18721 atcagaaccg gagtctgcta tgaaaaaatc gtggcgtaat aacgttgagt tttacttgat
    18781 cggcctgctg gtgctaactg ttgccgcgtt cagcattact atgccggaga ttttctggtc
    18841 gataagtaat ttccagtccg tcgcttcgca aatgcccgtg ctgggcattc tggcgttggc
    18901 tatggcggtg accatgcttt gcggtggcat taacttgtca attatagcca ctgctaacgc
    18961 ctgttcactg gtgatggcgt gggtggcgac tcagtatcca ccgggtattg ctacggttgt
    19021 cgctacgtta ctggcgggcg ctggcgcggc ggtgataatc ggtttatgta acggcgtatt
    19081 gattgctggt attcgagtgt cgccaattct tgcaacgctt gggatgatga cgttgctcaa
    19141 agggataaat attctggtga caggtggtag cgccattgcc aattatccct catgggtgct
    19201 gtggttgaat catgcgcaat ggttcggtat tccgctaccg atgtggttgt ttgccgcagt
    19261 ggcactgggg ctgtggatat tgctggagaa aacgccgctc ggtaaagcca tttacctgat
    19321 tgggtctaac gaacgagcaa cactttactc cggcattaac actcgtcggg tgctgatttg
    19381 ggtttatgtc atttccgcct tgctttgtgc ggtagcggca tttttgatga tgtcgaaact
    19441 caactcggct aaggcctctt atggcgaatc gtacttgctg gtgtcaatcc tcgcggcagt
    19501 actcggcggg gttaatccgg acggcggcag cgggcggata attggtatgg tgctggcgct
    19561 gtttcttctg caaattattg aaagcggctt caatattctc ggtataagcc cctacctgac
    19621 gatggcctta tggggaacgt tgctgctctg tttcattcag gttcgcggca tgttggggct
    19681 ggatcgggtg gtttaaatat tggctatatt caatggacgc gttttgccgc gatgacatat
    19741 caggcatcgc caaatacaca tagctaatca ggagtaaaca caatgaagat caaagctgtt
    19801 ggtgcatatt ccgctaaaca accacttgaa ccgatggata tcacacggcg tgaaccggga
    19861 ccgaatgatg tcaaaatcga aatcgcttac tgtggcgtct gccattccga tctccaccag
    19921 gtccgttccg agtgggcggg gacggtttac ccctgcgtac cgggtcatga aattgtgggg
    19981 cgtgtggtag ccgttggtga tcaggtagaa aaacatgcgc cgggcgatct ggtcggtgtc
    20041 ggctgcattg tcgacagttg taaacattgc gaagagtgtg aagacgggct ggaaaactac
    20101 tgtgatcaca tgaccggcac ctataactcg ccgacgccgg acgaaccggg ccatactctg
    20161 ggcggctact cacaacagat cgtcgttcat gagcgatatg ttctgcgtat tcgtcacccg
    20221 caagagcagc tggcggcggt ggctcctttg ttgtgtgcag ggatcaccac gtattcgccg
    20281 ctacgtcact ggcaggccgg gccgggtaaa aaagtgggcg tggtcggcat cggcggcctg
    20341 ggacatatgg ggattaagct ggcccacgcg atgggggcac atgtggtggc atttaccact
    20401 tctgaggcaa aacgcgaagc ggcaaaagcc ctgggggccg atgaagttgt taactcacgc
    20461 aatgccgatg agatggcggc tcatgtgaag agtttcgatt tcattttgaa tacagtagct
    20521 gcgccacata atctcgacga ttttaccacc ttgctgaagc gtgatggcac catgacgctg
    20581 gttggtgcgc ctgcgacacc gcataaatcg ccggaagttt tcaacctgat catgaaacgc
    20641 cgtgcgatag ccggttctat gattggcggc attccagaaa ctcaggagat gctcgatttt
    20701 tgcgccgaac atggcatcgt ggctgatata gagatgattc gggccgatca aattaatgaa
    20761 gcctatgagc gaatgctgcg tggtgatgtg aaatatcgtt ttgttatcga taatcgcaca
    20821 ctaacagact gaaaaaatta ataaatacgc tgtggtttaa catattaact tcgctctcca
    20881 cttaactttt tagttaagga gagcgaaata atatcaaagt agcagtaaaa cctataacgt
    20941 aaattcaaat tgttaaatta actccctcca gtacacaata cttcacacgt tagttatgag
    21001 cgatttctga tagtgcctgg tttaatgaga gctttattat ctgcgacgtt tatttttatt
    21061 taagagagat cgcgatgata tcattaaaag ctccgcacaa taatttgatg ccatatacgc
    21121 aacaaagcat acttaatacg gttaaaaaca atcagttacc agaggatatc aaaagctccc
    21181 tggtttcctg tgtcgatata ttcaaggttt tgattaaaca atattatgat tatccttatg
    21241 attgtcgtga tgatttagtc gatgacgaca aactcattca tctcatggct gccgtacgag
    21301 actgtgagtg gtcagacgat aacgcactca ccataaatgt gcagtttaat gatttccctg
    21361 gattttatga ctggatggat taccctgatc acccggttaa gtttgttttt cgcatacttg
    21421 aaaatcaaaa agggacagtt tgggtatatg accaggatga tgcgtttctt gatattaaag
    21481 caaatgtgca ggctggacgc tttaccgggc ttaagaaatt agtgcagttt atcgattcag
    21541 tgcgaacaga ttgcaaatgt attcttcttg aacaccatat gcctttgctg agaattttcc
    21601 ctaaagggaa agagtgtatg cgtgtggaaa aatggcttcg cgaaatgtcg tctattccgg
    21661 agacagacgc gcccattaag caggcgcttg cgcatggatt attattacac ttaaaaaata
    21721 tccaccctgt ttttcctgaa tcactggtga tgttattgct ctctgttctg gacgtaaaaa
    21781 catacagaga tgacgcacga ttaaatgaat ggatatcgaa tagagtgcag gaattaggtg
    21841 acagatatta tcctgtaaat aagcacgtta aaatccgcta cactctttag ttcagaaagg
    21901 gttccacctg caagttgtta ttccaccaga gagtgaatcg caaagcaggt aatcatttca
    21961 tctgtatatc cgtatgccaa atggcattcc accgcctttt tcgccgatat tttgttgtga
    22021 tcggcgacgc ttcgcttaaa aaagcaccag cagtggtttc gcagctatgc ggtgtataaa
    22081 aaatgatctc atgcagatgt tttgtgaatg tgtgggttga cattcatata aaaaaaatca
    22141 taattccatc atgtttgtgt ggggtctttt ctgtacctta gcatcgcact caagcctgac
    22201 agaaaatatg ctgtaaggct catatcaaaa accgccatta gctcatcagg aagagcagac
    22261 gacaaccagg attgttgtat ggtacggggt tcgaggcctc gatggcggtc caacttttca
    22321 aaactcttct gaatcagatc cccttattat catccctgca aagtggaaca ttgccattgt
    22381 ttggctattc aggacgggtc agaccaatga aaaaagccca cacaggggag agtgggctga
    22441 aatgggaagc taaagactca agtaaactta tcggaaataa ggaccacgca ttacgggggc
    22501 aatcatcgcc gatggtcggg attcggcaaa tcaggttgat tacgtagaga gtaaattatc
    22561 tgctcaccgc tgcgtcaccc cttcgtaaat caggcgtagc gcgaatacac caataattgc
    22621 accaataacc cgactggcaa cgcgttgcat acgcccataa gcacgacgca cagcgggcaa
    22681 agaaaacgcc tgactaagaa aaactcgcca gataattgat gcgagcacaa tccccgccca
    22741 ggccattaaa cgtgcccatg ttggtgtttc ggcatttaat gttactgaga aaatactgat
    22801 aaaaaataaa acggtttgcg ggttagagag atcggtaatt aatccgcggc gaaaaaagac
    22861 ataccagggg gcgctaatcg gttgttgtag tgtgctcatt tgcggtgttg actggcggcg
    22921 catgctgcac cacgcaaacc ataagagata agcgccgccg acgattctga taagcgaaaa
    22981 aatctcctca cactgcgtaa ttagcgttgc aagaccaaac aaacccaacc cggaataaaa
    23041 tgcatcgccc agcgccacgc ccagcccggt cagcacccct gcgcgtcgac cggaagccag
    23101 gctggtttgt actaccacaa agagattggc tcccggatta aaaaaagtaa tcacgaacag
    23161 tcctacggtc aggtaaacgg cgtgcaaagg atccatagtg atttcatcca taaataagtg
    23221 aagtaacttc atcatatttc taccaaaaat aatcgggtgc gagagagatc acaaagtgtc
    23281 ttatttccgg ttactggcgt ttatgccctg actgaactaa ttattaatca acccaataat
    23341 gtgggtggat gatagtgtga taacaactct ggagccgtaa tatgaaaata atctctaaaa
    23401 tgttagtcgg tgcgttagcg ttagccgtta ccaatgtcta tgccgctgaa ttgatgacca
    23461 aagcggaatt tgaaaaagtt gaatcgcagt atgaaaaaat aggtgatatt tcaactagca
    23521 atgaaatgtc gactgcagac gcgaaagaag atttgatcaa aaaagcggat gaaaaagggg
    23581 ctgatgtgtt ggtactgacc tccggtcaaa ctgacaataa gatccacggc acggcaaata
    23641 tttataagaa gaagtaattc tgaaccctat gtaaacatgc ccgatgcgta agtttatcgg
    23701 tgatcatcta ttgaaattta tgccggataa agcgttcgcg ctgcattcgg cagttcagct
    23761 tttcagccgc cgccagaacg tcgtccggct gatgcctaaa taattcgccg ctgctgtttt
    23821 atcgccatta aatttctcca gtgcctgttg tggtgtcagt aagcgtggag cgggagtttt
    23881 cgccgactcg cgcgccagtt ccggcagtag cagctgcaaa aattgcggcg ttaaatccgg
    23941 cgtcggttcc acacttaaaa atagcgccag tcgctccatc atattgcgca gttcacgaat
    24001 attgcccggc cagtcgtagt gcaccagcac ggtttcgctt gcctgtaatc ccaggcgtaa
    24061 tgcggcagaa aacggggcgg agagcgccgc cagtgacact ttcaaaaagc tttccgccag
    24121 cggcagaata tccgccaccc gctcacgcag tggtggcagt tgcaggcgta aaatgctcag
    24181 ccgataaaac aggtcacggc gaaactgccc ttgccgcata tcttcttcca gcttgcagtg
    24241 agtggcgcta atgacccgca catccaccgg aacaggctga tgcccgccga cgcgggtaac
    24301 ctctttttct tccagcaccc gcagcagccg ggtctgcaaa ggtagcggca tttcgccaat
    24361 ctcatccaaa aacagcgtgc caccgtgggc gatttcaaac agcccggcgc gacctccgcg
    24421 tcgcgagccg gtaaacgccc cttcctcata gccaaacagc tccgcttcca gcagcgattc
    24481 ggcaatcgcc ccgcagttga cggcaacaaa cggatgcgac tttttgccct gtcgcgcatc
    24541 gtggcgggca aagtattccc gatgaattgc ctgggctgcc agttctttgc ccgtccccgt
    24601 ttccccctca atcaacaccg ccgcactgga gcgggcatac agcaaaatag tctgccgcac
    24661 ctgttccatc tgtggtgatt gaccgagcat atcgcccagc acgtaacgag tacgcagggc
    24721 gttgcgagtg gcatcgtgag tgttatggcg taacgacatg cgcgtcatat ccagcgcatc
    24781 gctgaacgcc tggcgcacgg tggcggcgga atagataaaa attccggtca ttccggcttc
    24841 ttctgccaga tcggtaatca gccccgcgcc gaccaccgct tcggtgccgt tagcttttag
    24901 ctcgttaatc tgcccgcgtg cgtcttcttc ggtaatgtag ctacgttgat cgaggcgcaa
    24961 attaaaggtt ttttgaaacg ccaccagtgc cggaatagtt tcctgataag tgacaacgcc
    25021 gatagaagag gtgagttttc cggcttttgc cagtgcctgt aacacatcgt agccgctcgg
    25081 tttaatcaaa ataactggca ctgacaggcg gcttttcagg tacgcgccgt tagatccagc
    25141 cgcgatgatg gcgtcacagc gttcgtttgc cagtttcttg tggatgtagg tcaccgcttt
    25201 ttcaaagcca agctggatag gggtaatgtt cgccaggtga tcaaactcga ggctgatatc
    25261 gcgaaacagc tcgaacaggc gcgttacaga taccgtccag ataaccggtt tgtcgtcatt
    25321 aagccgtggt ggatgtgcca tagcgcaccg caaagttaag aaaccgaata ttgggtttag
    25381 tcttgtttca taattgttgc aatgaaacgc ggtgaaacat tgcctgaaac gttaactgaa
    25441 acgcatattt gcggattagt tcatgacttt atctctaaca aattgaaatt aaacatttaa
    25501 ttttattaag gcaattgtgg cacacccctt gctttgtctt tatcaacgca aataacaagt
    25561 tgataacaaa ggatgggcta tgtctctaca ctctccaggt aaagcgtttc gcgctgcact
    25621 gactaaagaa aatccattgc agattgttgg caccatcaac gctaatcatg cgctgttggc
    25681 gcagcgtgcc ggatatcagg caatttatct ttctggcggt ggcgtggcgg caggttcgct
    25741 ggggctgccc gatctcggta tttctactct tgatgacgtg ctgaccgata ttcgccgtat
    25801 caccgacgtt tgttcgctgc cgctgctggt ggatgcggat atcggttttg gttcttcggc
    25861 ctttaacgtg gcgcgcaccg tgaaatcgat gattaaagcc ggtgcggcag gattgcatat
    25921 tgaagatcag gttggtgcga aacgctgcgg tcatcgtccg aataaagcga tcgtctcgaa
    25981 agaagagatg gtggatcgga tccgcgcggc ggtggatgcg aaaaccgatc ctgattttgt
    26041 gatcatggcg cgcaccgatg ctctggcggt agaggggctg gatgcggcga tcgagcgtgc
    26101 gcaggcctat gttgaagcgg gtgccgagat gttgttcccg gaggcgatta ccgaactcgc
    26161 catgtaccgc cagtttgccg atgcggtgca ggtgccaatc ctcgccaaca ttaccgaatt
    26221 tggtgccacg ccgctgttta ccatcgacga attacgcagc gcccatgtcg caatggcgct
    26281 gtacccgctt tcagcgttcc gcgccatgaa ccgcgccgct gagcatgtct acaacgtcct
    26341 gcgtcaggaa ggcacgcaga aaagcgtcat cgataccatg caaacccgca acgagctgta
    26401 cgaaagcatc aactactacc agtacgaagc gaagctcgac gacctgtttg cccgtagcca
    26461 ggtgaaataa aacacaacga aaaatatctc gaccctacaa atgacgagga caacatgagc
    26521 gacacaacga tcctgcaaaa cagtacccat gtcattaaac cgaaaaaatc tgtggcactt
    26581 tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg gtaaaagtgg caatgacctg
    26641 cattaccgtg gctacgatat tcttgatctg gcggaacatt gcgaatttga agaagtggcg
    26701 cacctgctga tccacggcaa actgccgacc cgtgacgaac tcgccgctta caaaacgaaa
    26761 ctgaaagccc tgcgcggttt accggctaac gtgcgtaccg tgctggaagc cttaccggcg
    26821 gcgtcgtacc cgatggatgt catgcgcacc ggcgtttccg cgctcggctg cacactgcca
    26881 gaaaaagagg ggcacactgt ctctggcgcg cgggatattg ccgacaaact gctggcatca
    26941 cttagctcga ttctccttta ttggtatcac tacagtcaca acggcgaacg catccagccg
    27001 gaaaccgatg acgactccat cggtggtcac ttcctgcatc tgctgcacgg cgaaaagcca
    27061 tcgcaaagct gggaaaaggc gatgcatatt tcgctagtgc tgtacgccga acacgagttt
    27121 aacgcctcca cctttaccag tcgggtgatt gcgggcactg gctctgatat gtattccgcg
    27181 attattggcg cgattggcgc actgcgcggg ccgaaacacg gcggagcgaa tgaagtgtcg
    27241 ctggagatcc agcaacgcta cgaaacgccg gacgaagccg aagccgatat ccgcaagcgg
    27301 gtggaaaaca aagaagtggt cattggtttt ggtcatccgg tttataccat cgccgacccg
    27361 cgccaccagg tgatcaaacg tgtggcgaag cagctctcgc aggaaggcgg ctcgttgaag
    27421 atgtacaaca tcgccaatcg cctggaaacg gtgatgtggg agagcaaaaa gatgttcccc
    27481 aatctcgact ggttctctgc tgtttcctac aacatgatgg gcgttcccac cgagatgttc
    27541 acaccactgt ttgtcatcgc ccgtgtcacc ggctgggcgg cgcacatcat tgaacaacgt
    27601 caggacaaca aaattatccg tccttccgcc aattatgttg gaccggaaga ccgccagttt
    27661 gtcgcgctgg ataagcgcca gtaaacctct acgaataaca ataaggaaac atacccaatg
    27721 tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg aaatcgttga tatcgtcgat
    27781 tacgtgatga actacgaaat cagctctaaa gtggcctacg acaccgcaca ttactgcctg
    27841 ctcgacacgc tcggctgcgg tctggaagct ctcgaatacc cggcctgtaa aaaactgctg
    27901 gggccaattg ttcctggcac cgtcgtaccc aacggcgtgc gcgtccccgg aactcagttt
    27961 cagctcgacc ctgtccaggc ggcatttaac attggcgcga tgatccgctg gctcgatttc
    28021 aacgatacct ggctggcggc ggagtggggg catccttccg acaacctcgg cggcattctg
    28081 gcaacggcag actggctttc gcgcaacgct gtcgccagcg gcaaagcgcc gttgaccatg
    28141 aaacaggtgc tgaccgcaat gatcaaagcc catgaaattc agggctgcat cgcgctggaa
    28201 aactccttta accgcgtcgg cctcgaccac gttctgttag tgaaagtggc ttccaccgcc
    28261 gtggtcgccg aaatgctcgg cctgacccgc gaggaaattc tcaacgccgt ttcgctggcg
    28321 tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc cgaacaccgg cacgcgtaaa
    28381 tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc tggcactgat ggcgaaaacg
    28441 ggcgaaatgg gttacccgtc agccctgacc gcgccggtat ggggcttcta cgacgtctcc
    28501 tttaaaggtg aatcgttccg cttccagcgt ccgtacggtt cttacgtcat ggagaatgtg
    28561 ctgttcaaaa tctccttccc ggcggagttc cactcccaga cggcagttga agcggcgatg
    28621 acgctctatg aacagatgca ggcagcaggc aagacggcgg cggatatcga aaaagtgacc
    28681 attcgcaccc acgaagcctg tattcgcatc atcgacaaaa aggggccgct caataacccg
    28741 gctgaccgcg accactgcat tcagtacatg gtggcgatcc cgctgttatt cgggcgctta
    28801 acggcggcag attacgagga caacgttgcg caagataaac gcatcgacgc cctgcgcgag
    28861 aagatcaatt gctttgaaga tccggcattt accgctgact accacgaccc ggaaaaacgc
    28921 gccatcgcca atgccattac ccttgagttc accgacggca cacgatttga agaagtggtg
    28981 gtggagtacc ccattggtca tgctcgccgc cgtcaggatg gtattccgaa actggtcgat
    29041 aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc aacagcagcg cattctggag
    29101 gtttctctcg acagaactcg cctggaacag atgccggtca atgagtatct cgacctgtac
    29161 gtcatttaag taaactgcgg taaggcgtaa gttcaacagg agagcattat gtcttttagc
    29221 gaattttatc agcgttcgat taacgaaccg gagcagttct gggccgagca ggcccggcgt
    29281 attgactggc agacgccctt tacgcaaacg ctcgaccaca gcaatccgcc gtttgcccgt
    29341 tggttttgtg aaggccgaac caatttgtgc cacaacgcta tcgaccgctg gctggagaaa
    29401 cagccagagg cactggcatt gattgccgtc tcttcggaaa cagaagaaga gcgcaccttt
    29461 acctttcgcc agctgcatga cgaagtgaac gcggtggcct caatgttgcg ctcactgggc
    29521 gtgcagcgcg gcgatcgggt gctggtgtat atgccgatga ttgccgaagc gcatattact
    29581 ctgctggcct gcgcgcgcat tggtgctatt cattcggtgg tatttggtgg ttttgcctcg
    29641 cacagcgtgg cggcgcgaat tgatgacgct aaaccggtgc tgattgtctc ggctgatgcc
    29701 ggagcgcgcg gtggcaaaat cattccctat aaaaaattgc tcgacgatgc gataagtcag
    29761 gcgcagcacc agccacgcca tgttttgctg gtggatcgcg ggctggcgaa aatggcgcgc
    29821 gtcagcgggc gggatgtcga tttcgcgtcg ttgcgccatc aacacatcgg cgcgcgggta
    29881 ccggtggcgt ggctggaatc caacgaaacc tcctgcattc tctacacttc cggcacgacc
    29941 ggcaaaccta aaggcgtgca gcgtgacgtc ggcggatatg cggtggcgct ggcgacctcg
    30001 atggacacca tttttggcgg caaagcgggc agcgtgttct tttgcgcatc ggatatcggc
    30061 tgggtggtgg ggcattcgta tatcgtttac gcgccgctgc tggcggggat ggcgactatc
    30121 gtttacgaag gattgccgac ctggccggac tgcggcgtgt ggtggaaaat cgtcgagaaa
    30181 tatcaggtta gccggatgtt ctcagcgccg accgccattc gcgtgctgaa aaaattccct
    30241 accgctgaaa ttcgcaaaca cgatctctcg tcgctggaag tgctctatct ggctggagaa
    30301 ccgctggacg agccgaccgc cagttgggtg agcaatacgc tggatgtgcc ggtcatcgac
    30361 aactactggc agaccgaatc cggctggccg attatggcga ttgctcgcgg tctggacgac
    30421 aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg gctataacgt gcagttgctt
    30481 aatgaagtca ccggcgaacc gtgtggcgtc aacgagaaag ggatgctggt ggtggaaggg
    30541 ccgctgccgc cgggctgtat tcagaccatc tggggcgacg acgaccgctt tgtgaagact
    30601 tactggtcgc tgttttcccg cccggtgtac gccacctttg actggggcat ccgtgacgct
    30661 gacggttatc actttattct cgggcgcact gacgatgtaa ttaacgttgc cgggcatcgt
    30721 ctgggcacgc gcgagattga agagagtatc tccagccatc cgggcgttgc cgaagtggcg
    30781 gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg tggcgtttgt cattccgaaa
    30841 gagagcgata gtctggaaga tcgtgatgtg gcgcattcgc aagagaaggc gattatggcg
    30901 ctggtggaca gccagattgg caactttggt cgcccggcgc acgtctggtt tgtctcgcaa
    30961 ttgccgaaaa cgcgatccgg aaaaatgctg cgccgcacga tccaggcaat ttgcgaagga
    31021 cgcgatcctg gggatctgac gaccattgat gatcctgcgt cattggatca gatccgccag
    31081 gcgatggaag agtaaggtat tgtcggatgc gtcgcgtggc gcatccgaca ccatgcgccg
    31141 atgcctgatg cgacgcttac cgcgtcttat tatgcctaca ggcctgaacc gtaggtcgga
    31201 taaggcgctc gcgtcgcatc cgacaccccg cgccgatgcc tgatgcgacg ctgacgcgtc
    31261 ttatcagtat ctacccacta cccactgttt ttacaccgat aatttttccc ccaccttttt
    31321 gcactcattc atataaaaaa tatatttccc cacgaaaacg attgcttttt atcttcagat
    31381 gaatagaata cggcggattt tttgggtttc aaacagcaaa aagggggaat ttcgtgtcgc
    31441 aagataacaa ctttagccag gggccagtcc cgcagtcggc gcggaaaggg gtattggcat
    31501 tgacgttcgt catgctggga ttaaccttct tttccgccag tatgtggacc ggcggcactc
    31561 tcggaaccgg tcttagctat catgatttct tcctcgcagt tctcatcggt aatcttctcc
    31621 tcggtattta cacttcattt ctcggttaca ttggcgcaaa aaccggcctg accactcatc
    31681 ttcttgctcg cttctcgttt ggtgttaaag gctcatggct gccttcactg ctactgggcg
    31741 gaactcaggt tggctggttt ggcgtcggtg tggcgatgtt tgccattccg gtgggtaagg
    31801 caaccgggct ggatattaat ttgctgattg ccgtttccgg tttactgatg accgtcaccg
    31861 tcttttttgg catttcggcg ctgacggttc tttcggtgat tgcggttccg gctatcgcct
    31921 gcctgggcgg ttattccgtg tggctggctg ttaacggcat gggcggcctg gacgcattaa
    31981 aagcggtcgt tcccgcacaa ccgttagatt tcaatgtcgc gctggcgctg gttgtggggt
    32041 catttatcag tgcgggtacg ctcaccgctg actttgtccg gtttggtcgc aatgccaaac
    32101 tggcggtgct ggtggcgatg gtggcctttt tcctcggcaa ctcgttgatg tttattttcg
    32161 gtgcagcggg cgctgcggca ctgggcatgg cggatatctc tgatgtgatg attgctcagg
    32221 gcctgctgct gcctgcgatt gtggtgctgg ggctgaatat ctggaccacc aacgataacg
    32281 cactctatgc gtcgggttta ggtttcgcca acattaccgg tatgtcgagc aaaacccttt
    32341 cggtaatcaa cggtattatc ggtacggtct gcgcattatg gctgtataac aattttgtcg
    32401 gctggttgac cttcctttcg gcagctattc ctccagtagg gggcgtgatc atcgccgact
    32461 atctgatgaa ccgtcgccgc tatgagcact ttgcgaccac gcgtatgatg agtgtcaatt
    32521 gggtggcgat tctggcggtc gcgctgggga ttgccgcagg ccactggtta ccgggaattg
    32581 ttccggtcaa cgcggtatta ggtggcgcgc tgagctatct gatccttaac ccgattttga
    32641 atcgtaaaac gacagcagca atgacgcatg tggaggttaa cagtgtcgaa taacgcttta
    32701 caaacaatta ttaacgcccg gttaccaggc aaagaggggc tgtggcagat tcatctgcag
    32761 gacggaaaaa tcagcgccat tgatgcgcaa tccggcgtga tgcccataac tgaaaacagc
    32821 ctggatgccg aacaaggttt agttataccg ccgtttgtgg agccgcatat tcacctggac
    32881 accacgcaaa ccgccggaca accgaactgg aatcagtccg gcacgctgtt tgaaggcatt
    32941 gaacgctggg ccgagcgcaa agcgttatta acccatgacg atgtgaaaca acgcgcctgg
    33001 caaacgctga aatggcagat tgccaacggc attcagcatg tgcgtaccca tgtcgatgtt
    33061 tcggatgcaa cgctaactgc gctgaaagca atgctggaag tgaagcagga agtcgcgccg
    33121 tggattgatc tgcaaattgt cgccttccct caggaaggga ttttgtcgta tcccaacggt
    33181 gaagcgttgc tggaagaggc gttacgctta ggggcagatg tagtgggggc gattccgcat
    33241 tttgaattta cccgtgaata cggcgtggag tcgctgcata aaaccttcgc cctggcgcaa
    33301 aaatacgacc gtctcatcga cgttcactgt gatgagatcg atgacgagca gtcgcgcttt
    33361 gtcgaaaccg ttgctgccct ggcgcaccgt gaaggcatgg gcgcgcgagt caccgccagc
    33421 cacaccacgg caatgcactc ctataacggg gcgtatacct cacgcctgtt ccgcttgctg
    33481 aaaatgtccg gtattaactt tgtcgccaac ccgctggtca atattcatct gcaaggacgt
    33541 ttcgatacgt atccaaaacg tcgcggcatc acgcgcgtta aagagatgct ggagtccggc
    33601 attaacgtct gctttggtca cgatgatgtc ttcgatccgt ggtatccgct gggaacggcg
    33661 aatatgctgc aagtgctgca tatggggctg catgtttgcc agttgatggg ctacgggcag
    33721 attaacgatg gcctgaattt aatcacccac cacagtgcca gaacattgaa tttgcaggat
    33781 tacggcattg ccgccggaaa cagcgcaaac ctgattatcc tgccggctga aaatggattt
    33841 gatgcgctgc gccgtcaggt tccggtacgt tattcggtac gtggcggcaa ggtgattgcc
    33901 agtacacaac cggcacaaac caccgtatat ctggagcagc cagaagccat cgattacaaa
    33961 cggtaatttc ctctgtgttg aagagccact gacctgtgtc agtggctacc gtgattcatt
    34021 ctcgccaaca tccgcacatt cctccaacgc catgtgcaaa aatgccttcg cggcggctgt
    34081 ctgccagcta tttttccgcc gcaacaaaac cgccgttctc tccagtagtg gcggggcaag
    34141 agaaatcgct ttaagcccgt catgttgtgt ggcaatcgct gctggtaaca atgtggaaag
    34201 ggaagtgcgg cgaatcagct ccagaaccgc gctaattgag ttcgcctcaa tgaccacctg
    34261 tggatgtagc cccgctttct cgcagtagtg gtcaatttgc tctctggtgg caaattccgc
    34321 gctgagcagg accagttttt catcatgcaa gcgactcaac gccacctgtt cacaggcagc
    34381 cagcggatga tgttgcgcca cgactaacgc taaactttct gtcagtaaag gaattgcctc
    34441 cagctccggc gaatgcacag gcgcgaaggc aatcccaacg tccagctcgt cgcggcaaag
    34501 cagatcttcg attttctcct gcgacatttc ctgtagctgg agcgtgatgc cggggtagcg
    34561 cgcatagaaa tccgccatta aagggccgat aaagtagctt gtaaaggtgg gggtgacggc
    34621 gatacgcagc gatcctcgcg tcagatcggc aacatcatga atcgcccgtt tacccgctcc
    34681 cagttcctgt aacgcccggc tggcgtactg tcgccagact tctcctgcat cggtgagacg
    34741 aatcgttcgc ccgctacggt caaacagcgg cacgcctaaa ctctcctcta actggcgaat
    34801 ctgctgggaa agcgcaggct gagagacgtg caacgcactg gcggcacggg tgaagctgcc
    34861 atgttcagcc acggcaagaa aataattgat atgtcgagag agcattcgcc aacctataag
    34921 taaatccaat agaactcatc ataaatgaga cttttacctt atgacaatcg gcgagtagtc
    34981 tgcctctcat tccagagaca gacagaggtt aacggtgaaa gagattattg atggattcct
    35041 taaattccag cgcgaggcat ttccgaagcg ggaagccttg tttaaacagc tggcgacaca
    35101 gcaaagcccg cgcacacttt ttatctcctg ctccgacagc cgtctggtcc ctgagctggt
    35161 gacgcaacgt gagcctggcg atctgttcgt tattcgcaac gcgggcaata tcgtcccttc
    35221 ctacgggccg gaacccggtg gcgtttctgc ttcggtggag tatgccgtcg ctgcgcttcg
    35281 ggtatctgac attgtgattt gtggtcattc caactgtggc gcgatgaccg ccattgccag
    35341 ctgtcagtgc atggaccata tgcctgccgt ctcccactgg ctgcgttatg ccgattcagc
    35401 ccgcgtcgtt aatgaggcgc gcccgcattc cgatttaccg tcaaaagctg cggcgatggt
    35461 acgtgaaaac gtcattgctc agttggctaa tttgcaaact catccatcgg tgcgcctggc
    35521 gctcgaagag gggcggatcg ccctgcacgg ctgggtctac gacattgaaa gcggcagcat
    35581 cgcagctttt gacggcgcaa cccgccagtt tgtgccactg gccgctaatc ctcgcgtttg
    35641 tgccataccg ctacgccaac cgaccgcagc gtaaccttat ttttaaacca tcaggagatc
    35701 caccatgatt cagtcacaaa ttaaccgcaa tattcgtctt gatcttgccg atgccatttt
    35761 gctcagcaaa gctaaaaaag atctctcatt tgccgagatt gccgacggta ccggtctggc
    35821 agaagccttc gtaaccgcgg ctttgctggg gcagcaagcg cttcctgccg acgccgcccg
    35881 cctggtcggg gcgaagctgg atctcgacga agacgccatt ctactgttgc agatgattcc
    35941 actgcgtggc tgcattgatg accgtattcc aaccgaccca acgatgtatc gtttctatga
    36001 aatgttgcag gtgtacggca caaccctgaa agcgttggtt catgagaagt ttggcgatgg
    36061 cattatcagc gcgattaact tcaaactcga cgttaagaaa gtggcggacc cggaaggtgg
    36121 cgaacgtgcg gtcattacct tagatggtaa atatctgccg accaagccgt tctgacagcc
    36181 atgcgcaatc atcaaaagac gttcacgatg ctgctggttc tggtgctgat tggtcttaat
    36241 atgcgaccac tgctcacctc cgtcgggcca ctgctaccgc aattgcgcca ggcgagcgga
    36301 atgagcttta gcgtggctgc cctgttgacc gctcttccgg tggtcaccat gggcgggctg
    36361 gcgctggccg gaagctggct tcatcagcat gtcagcgaac gtcgcagtgt cgccatcagt
    36421 ctgttgctga ttgccgtcgg tgcattgatg cgtgagcttt acccccaaag tgtgctgctg
    36481 cttagcagcg cactgcttgg tggggtgggg atcggtatca ttcaggcggt gatgccttca
    36541 gtgattaaac ggcggtttca gcaacgcacg ccactggtga tggggctgtg gtctgcggct
    36601 ctgatgggcg gcggtggact tggtgccgcc ataactccct ggttagttca acatagcgaa
    36661 acctggtatc aaacactcgc ctggtgggcg ctgcctgccg ttgttgcgct ctttgcctgg
    36721 tggtggcaaa gcgcccgcga ggtcgcctct tcccacaaga caacaaccac tccggttcgc
    36781 gtggtattca ctccccgcgc gtggacgctg ggtgtttact tcggtctgat taacggcggt
    36841 tacgccagcc tgattgcctg gttacccgct ttctatattg agattggtgc cagcgcgcag
    36901 tacagcggtt ccttactggc attgatgacg cttgggcaag ccgcaggagc tttgctgatg
    36961 cctgctatgg ctcgccatca ggatcggcgc aaactgttaa tgctggcgct ggtgttacaa
    37021 ctggtggggt tctgcggctt tatctggttg ccgctgcaat tgccggtatc gtgggcgatg
    37081 gtgtgtgggt taggtctggg cggcgcgttt ccgctctgtt tgctgctggc gctcgatcac
    37141 tctgtgcaac cggctattgc tggcaagctg gtggcgttta tgcaggggat cggttttatc
    37201 atcgccgggc ttgccccgtg gttttctggc gtgctgcgta gtatcagcgg caattacctg
    37261 atggactggg cgtttcatgc gctgtgcgtc gttgggctga tgatcataac cctgcgtttt
    37321 gcaccagcac gttttccgca gctgtgggtc aaagaggtat gatgcgacgc ttgtttcgcc
    37381 agatgatgga gctataaata attgtcggat gtgcccagtg gcgcatccga cattaattgc
    37441 ttaagcgact tcattcacct gacgacgcag cagggaaagc gggccggggc cgctaagcgt
    37501 gaacacggaa attaaggtga agcccagcgc caccagaccc agcaccagat aagcgccctg
    37561 gaaaccgatg ctttcataca tattgcccgc cagtacagac ataaaaatca tcgccagttg
    37621 cttaaagaag cagaaacaga ccagataaat cgtcgctgaa aaacgcactt caaactggct
    37681 ggtaatatat ttaaagcagc ccaccagcag gaacggtact tcaaacatat gcagcgtttt
    37741 cagaataacc acttccagcg ctgaggtggc gaacgatgag ccaataatac gtacagacat
    37801 aatagtgcca gccagcagca gggcgttttt cccaccgatg cgattaatga tcagtggcgc
    37861 aaagaacata atcgaggcgt taagtaattc gcccattgtc gttacgtagc caaatacccg
    37921 cgtaccctgt tcaccggtag caaagaacga agtaaagaaa ttagcaaact gttggtcaaa
    37981 aacatcgtag gtgcaggaaa cgccaataac atacagtgac aaaaaccaca gttttggctg
    38041 tctgaacagt tccagtgcca gcttaaggct aaatgccgaa tggttggcac ctaccgcatt
    38101 ggcaaccgtg gcggaagagg gcgcatccgt tttggcgaaa aagagtaaaa cggcgaggat
    38161 gagtgcacag ccagaaccca gccagaaaac aaactgatta ttgatggtga acatgatgcc
    38221 gacaatcgag gcacacaacg cccagccaac acagccaaac atccgcgcgc gaccaaattc
    38281 gaaattactg cgacggctga ctttctcaat aaatgcctct actgctggcg caccggcgtt
    38341 aaaacaaaag cctagataaa taccaccaac aatcgatcct actaaaatgt tgtattgtaa
    38401 cagtggcccg aagataaaaa taaagaacgg cgcaaacatc actaacatgc cggtaataat
    38461 ccacagcagg tatttgcgca gcccgagttt gtcagaaagc agaccaaaca gcggttggaa
    38521 taatagcgag aacagagaaa tagcggcaaa aataataccc gtatcacttt tgctgatatg
    38581 gttgatgtca tgtagccaaa tcgggaaaaa cgggaagtag gctcccatga taaaaaagta
    38641 aaagaaaaag aataaaccga acatccaaaa gtttgtgttt tttaaatagt acataatgga
    38701 tttccttacg cgaaatacgg gcagacatgg cctgcccggt tattattatt tttgacacca
    38761 gaccaactgg taatggtagc gaccggcgct cagctggaat tccgccgaca ctgacgggct
    38821 ccaggagtcg tcgccaccaa tccccatatg gaaaccgtcg atattcagcc atgtgccttc
    38881 ttccgcgtgc agcagatggc gatggctggt ttccatcagt tgctgttgac tgtagcggct
    38941 gatgttgaac tggaagtcgc cgcgccactg gtgtgggcca taattcaatt cgcgcgtccc
    39001 gcagcgcaga ccgttttcgc tcgggaagac gtacggggta tacatgtctg acaatggcag
    39061 atcccagcgg tcaaaacagg ccgcagtaag gcggtcggga tagttttctt gcggccctaa
    39121 tccgagccag tttacccgct ctgcgacctg cgccagctgg caggtcaggc caatccgtgc
    39181 cggatgcggt gtatcgctcg ctacttcaac atcaacggta atcgccattt gaccactacc
    39241 atcaatccgg taggttttcc ggctgataaa taaggttttt ccctgatgct gccacgcgtg
    39301 agcggtcgta atcagcaccg cgtcggcaag tgtatctgcc gagcactgca acaacgctgc
    39361 ttcggcctgg taatggcccg ccgccttcca gcgttcgacc caggcgttag ggtcaatgcg
    39421 ggtcgcttca cttacgccaa tgtcgttatc cagcggtgca cgggtgaact gatcgcgcag
    39481 cggcgtcagc agttgttttt catcgccaat ccacatctgt gaaagaaggc ctgactgacg
    39541 gttaaattgc caacgcttat tacccagctc gatgcaaaaa tccatttcgc tggtggtcag
    39601 atgcgggatg gcgtgggacg cggcggggag tgtcacgctg aggttttccg ccagacgcca
    39661 ctgctgccag gcgctgatgt gtccggcttc tgaccatgcg gtcgcgttcg gttgcactac
    39721 gtgtaccgta agccagagtt gtccggcgct ctccggctgc ggtagtccag gcagttcaat
    39781 caactgttta ccttgtggag cgacatccag aggcacttca ccgcttgcca gcggcttgcc
    39841 atccagcgcc accatccagt gcaggagctc gttatcgcta tgacggaaca ggtattcgct
    39901 ggtcacttcg atggtttgcc cggataaacg gaactggaaa aactgctgct ggtattttgc
    39961 ttccgtcagc gccggatgcg gcgtgcggtc ggcaaagacc agaccgttca tgcagaactg
    40021 gcgatcgttc ggcgtatcgc caaaatcacc gccgtaagcc aaccacgggt tgccgttttc
    40081 atcatattta atcagcgact gatccaccca gtcccagacg aagccgccct gtaaacgggg
    40141 atactgacga aacgcctgcc agtatttagc gaaaccgcca agactgttac ccattgcgtg
    40201 ggcatattcg caaaggatca gtgggcgcag ttctccaggc agcgaaagcc attttttgat
    40261 ggaccatttc ggcaccgccg ggaagggttg gtcttcatcc acgcgcgcgt acatcgggca
    40321 aataatatcg gttgcgaagg tgtcggctcc gccgccttca tactgcaccg ggcgggaagg
    40381 atcgacagat ttgatccagc gatacagcgc gtcgtgatta gcgccgtggc ctgactcatt
    40441 ccccagcgac cagatgatca cactcgggtg attacgatcg cgctgcacca ttcgcgttac
    40501 gcgttcgctc atcgccggta gccagcgcgg atcatcggtc agacgattca ttggcaccat
    40561 gccgtgggtt tcaatattgg cttcatccac cacatacagg ccgtagcggt cgcacagcgt
    40621 gtaccacagc ggatggttcg gataatgcga acagcgcacg gcgttaaagt tgttctgctt
    40681 catcagcagg atatcctgca ccatcgtctg ctcatccatg acctgaccat gcagaggatg
    40741 atgctcgtga cggttaacgc ctcgaatcag caacggcttg ccgttcagca gcagcagacc
    40801 attttcaatc cgcacctcgc ggaaaccgac atcgcaggct tctgcttcaa tcagcgtgcc
    40861 gtcggcggtg tgcagttcaa ccaccgcacg atagagattc gggatttcgg cgctccacag
    40921 tttcgggttt tcgacgttca gacgtagtgt gacgcgatcg gcataactac cacgctcatc
    40981 gataatttca ccgccgaaag gcgcggtgcc gctggcgacc tgcgtttcac cctgccataa
    41041 agaaactgtt acccgtaggt agtcacgcaa ctcgccgcac atctgaactt cagcctccag
    41101 tacagcgcgg ctgaaatcat cattaaagcg agtggcaaca tggaaatcgc tgatttgtgt
    41161 agtcggttta tgcagcaacg agacgtcacg gaaaatgccg ctcatccgcc acatatcctg
    41221 atcttccaga taactgccgt cactccagcg cagcaccatc accgcgaggc ggttttctcc
    41281 ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa cgactgtcct gaccgtaacc
    41341 gacccagcga ccgttgcacc acagatgaaa cgccgagtta acgccatcaa aaataattcg
    41401 cgtctggcct tcctgtagcc agctttcatc aacattaaat gtgagcgagt aacaacccgt
    41461 cggattctcc gtgggaacaa acggcggatt gaccgtaatg ggataggtca cgttggtgta
    41521 gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg acgacgacag tatcggcctc
    41581 aggaagatcg cactccagcc agctttccgg caccgcttct ggtgccggaa accaggcaaa
    41641 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcttcttc
    41701 gctattacgc cagctggcga aagggggatg tgccgcaagg cgattaagtt gtgtaacgcc
    41761 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaatccgtaa tcatggtcat
    41821 agctgtttcc tgtgtgaaat tgttatccgc tcacaatttc acacaacata cgagccggaa
    41881 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    41941 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    42001 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
    42061 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
    42121 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
    42181 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
    42241 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
    42301 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
    42361 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
    42421 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
    42481 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
    42541 gagaaaataa tactgttgat gggagtctgg tcagagacat caagaaataa cgccggaaca
    42601 ttagcgcagg ctgcttccac agcagtggca tcctggtcat ccagcggata gttaatgagc
    42661 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
    42721 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcagcgcg agatttaatc
    42781 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
    42841 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
    42901 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
    42961 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtatag cgttactggt
    43021 ttcacattca ccaccctgaa ttgactctct tccgaacgct atcatgccat accgcgaaag
    43081 gttttgcgcc attcgatggt gtcaacgtaa atgcacgccg cttcgccgtc cggccaccag
    43141 aatagcctgc gattcaaccc cttcttcgat ctgttttgct acccgttgta gcgccggaag
    43201 atgcttttcc gctgcctgtt caatggtcat tgcgctcgcc atatacacca gattcagaca
    43261 gccaatcacc cgttgttcac tgcgcagcgg tacggcgata gaggcgatct tctcctcctg
    43321 atcccagccg cggtagttct gtccgtaacc ctctttgcgc gcgcgcgcca gaatggcttc
    43381 cagctttaac ggttcccgtg ccagttgata gtcatcaccg gggcgggagg ctaacatttc
    43441 gattaattcc ttgcggtctt gttccgggca aaaggccagc caggtcaggc ccgaggcggt
    43501 tttcagaagc ggcaaacgtc gcccgaccat tgcccggtga aaggataagc ggctgaaacg
    43561 gtgagtggtt tcgcgtacca ccattgcatc aacatccagc gtggacacat ctgtcggcca
    43621 taccacttcg cgcaacagat cgcccagcag tggggccgcc agtgcagaaa tccactgttc
    43681 gtcacgaaat ccttcgctta attgccgcac tttgatggtc agtcgaaaac tatcatcgga
    43741 ggggctacgg cggacatatc cctcttcctg cagcgtctcc agcagtcgcc gcacagtggt
    43801 gcgatgcagg ccgctgagtt ccgccagcag cccgacgctg gcaccgccat caagtttatt
    43861 taacatattt aataacatta gaccgcgggt taagccgcgc acggttttgt attccgtctg
    43921 ctcattgttc tgcatattaa ttgatatttc tatagttaaa acaacgttgt gcacctggtg
    43981 cacattcggg catgttttga ttgtagccga aaacaccctt cctatactga gcgcacaata
    44041 aaaaatcatt tacatgtttt taacaaaata agttgcgccg tactgtgcgc gcaacggcat
    44101 tttgtccgag tcgtgaggta ctgaaatggc aatacaacac cctgacatcc agcctgctgt
    44161 taaccatagc gttcaggtgg cgatcgcagg tgccggtccg gtcgggctga tgatggcgaa
    44221 ctatctcggt cagatgggca ttgacgtgct ggtggtggag aaactcgata agttgatcga
    44281 ctacccgcgt gcgattggta ttgatgacga ggcgctgcgc accatgcagt cggttggcct
    44341 ggtcgagaat gttctgccgc acactacgcc gtggcacgcg atgcgttttc tcaccccgaa
    44401 aggccgctgt tttgctgata ttcagccaat gaccgatgaa tttggctggc cgcgccgtaa
    44461 cgcctttatt cagccgcagg tcgatgcggt gatgctggaa ggggtgtcgc gttttcctaa
    44521 tgtgcgctgc ttgttttccc gcgagctgga ggccttcagc cagcaaaatg acgaagtgac
    44581 cttgcacctg aaaacggcag aagggcagcg ggaaatagtc aaagcccagt ggctggtagc
    44641 ctgtgacggt ggagcaagtt ttgtccgtcg cactctgaat gtgccgtttg aaggtaaaac
    44701 tgcgccaaat cagtggattg tggtagatat cgccaacgat ccgttaagta cgccgcatat
    44761 ctatttgtgt tgcgatccgg tgcgcccgta tgtttctgcc gcgctgcctc atgcggtacg
    44821 tcgctttgaa tttatggtga tgccgggaga aaccgaagag cagctgcgtg agccgcaaaa
    44881 tatgcgcaag ctgttaagca aagtgctgcc taatccggac aatgttgaat tgattcgcca
    44941 gcgtgtctac acccataacg cgcgactggc gcaacgtttc cgtattgatc gcgtactgct
    45001 ggcgggcgat gccgcgcaca tcatgccggt gtggcagggg cagggctata acagtggtat
    45061 gcgcgacgcc tttaacctcg cctggaaact ggcgttggtt atccagggga aagcccgcga
    45121 tgtgctgctc gatacctatc aacaagaacg tcgcgatcac gccaaagcga tgattgacct
    45181 gtccgtgacg gcgggcaacg tgctggctcc gccgaaacgc tggcagggta cgttacgtga
    45241 cggcgtttcc tggctactga attatctgcc gccagtaaaa cgctacttcc tcgaaatgcg
    45301 cttcaagccg atgccgcaat attacggcgg tgcgctggta cgtgagggcg aagcgaagca
    45361 ctctccggtc ggcaagatgt ttattcagcc gaaagtcacg ctggaaaacg gcgacgtgac
    45421 gctgctcgat aacgcgattg gcgcgaactt cgcggtaatt ggctggggat gcaatccact
    45481 gtgggggatg agcgacgagc aaatccagca gtggcgcgcg ttgggcacac gcttcattca
    45541 ggtggtgccg gaagtgcaaa ttcataccgc acaggataac cacgacggcg tgctacgcgt
    45601 gggcgatacg caaggtcgtc tgcgtagctg gttcgcgcaa cacaatgctt cgctggtggt
    45661 gatgcgcccg gatcgctttg ttaccgccac cgccattccg caaaccctgg gcaatacgct
    45721 gaataaactg gcgtcggtga tgacgctgac ccgccctgat gccgacgttt ctgtcgaaaa
    45781 ggtagcctga tatgcacgct tatcttcact gtctttccca ctcgccgttg gtggggtatg
    45841 tcgacccggc gcaagaggta ctcgatgagg tcaatggcgt gattgccagc gcccgcgagc
    45901 gtattgcggc tttctcccct gaactggtgg tgctgtttgc gccagatcac tacaacggct
    45961 ttttctatga cgtgatgcca ccgttctgtt taggcgttgg agcgacggca attggtgatt
    46021 tcggcagtgc ggcaggagag ctgcccgtgc ctgtggagct ggcggaggcc tgtgcgcatg
    46081 ctgtcatgaa gagcgggatc gatcttgccg tttcttactg tatgcaggtg gaccacgggt
    46141 tcgcccagcc gctggagttc ctgctcggtg ggctggataa ggtgccagtt ctgcctgtgt
    46201 tcatcaacgg tgtcgccacg ccgctgcccg gtttccagcg tacccgcatg ttgggtgaag
    46261 ccattggacg tttcaccagc actctcaata aacgcgtgct gttcctgggt tccggtgggc
    46321 tttcccatca gccgccggtg cccgaactgg cgaaagccga tgcccatatg cgcgaccgtc
    46381 tgttggggag cgggaaagat ttacccgcca gtgagcgcga attgcgtcag caacgggtga
    46441 ttagcgccgc tgagaagttt gttgaggatc agagaactct gcatccgctc aacccgattt
    46501 gggataacca gttcatgact ttgctggagc agggacgcat acaggaactg gatgccgtca
    46561 gtaacgaaga gctttccgcc attgccggaa agtcgacaca tgaaatcaaa acctgggtcg
    46621 ccgcttttgc cgctatttct acatttggca actggcgtag cgaagggcgt tattaccgcc
    46681 caatcccgga gtggattgcc ggatttggct cgttaagcgc cagaacagag aactgaatat
    46741 gcaggagaag atgatgagtt atcagccaca aaccgaagcc gccaccagcc gttttctgaa
    46801 tgtagaagaa gcgggtaaaa cgctgcgcat ccattttaat gactgcggac aaggcgacga
    46861 aaccgttgtc ctgctgcatg gttccggccc gggtgctact ggctgggcga acttcagccg
    46921 caatatcgat ccgctggtag aggcgggcta tcgggtaatc ctgctggatt gtccgggttg
    46981 gggcaaaagc gattcgatcg ttaatagtgg ttcacgatcg gatcttaatg cacgaatcct
    47041 gaaaagcgtg gtggatcaac tggatatcgc caaaatccac ctgctgggca actcgatggg
    47101 cggccatagt tctgtggcgt tcacccttaa ctggccggag cgcgtcggca aactggtgct
    47161 gatgggcggc ggtacgggcg gcatgagttt gtttacgccg atgccaaccg aaggtattaa
    47221 gcgactgaat cagctttatc gtcagccgac tatcgaaaac ctgaagctga tgatggatat
    47281 cttcgttttc gataccagcg atttgaccga cgccctgttt gaagcgcgcc tgaataatat
    47341 gctgtcgcgc cgcgatcacc aggaaaactt cgttaagagc ctggaagcta atccgaaaca
    47401 gttcccggat tttggcccac gtctggcgga aatcaaagcg caaaccctga ttgtctgggg
    47461 gcgcaacgac cgctttgtgc cgatggatgc gggtctgcgt ctgctgtccg gcattgccgg
    47521 ttctgaactg catatcttcc gcgactgtgg tcactgggcg cagtgggaac atgccgacgc
    47581 tttcaatcaa ttggtgctga atttcctcgc acgcccttaa ggaatggtca tgacgaagca
    47641 tactcttgag caactggcgg cggatttacg ccgcgccgca gagcagggcg aagcgattgc
    47701 gccgctgcgc gatctgattg gtatcgataa cgctgaagcg gcctacgcca ttcagcacat
    47761 aaatgtgcaa tatgacgttg tgcaggggcg tcgcgtggta gggcgtaaag tgggcctgac
    47821 gcatccgaaa gtgcaacaac aactgggcgt tgatcaaccg gattttggga cgttatttgc
    47881 cgacatgtgt tatggcgata acgaaatcat tcctttttcc cgtgttctgc aaccccgcat
    47941 tgaagcggag atcgcactgg tgttgaaccg cgatttaccc gcaactgata tcaccttcga
    48001 cgaactgtat aacgccattg aatgggtact tccggcgctg gaagtggtgg ggagccgcat
    48061 tcgcgactgg tcgattcagt ttgtcgatac cgtggcagat aacgcttcct gcggggtgta
    48121 tgtcatcggc ggtccggcgc aacgtccggc ggggttagac ctgaaaaact gcgccatgaa
    48181 gatgacgcgt aataacgaag aggtttccag cgggcgcggc agcgaatgcc tgggacatcc
    48241 gcttaatgcc gccgtctggc tggcacgcaa aatggccagt ctgggtgaac cgctgcgcgc
    48301 cggagatatc attcttaccg gggcattagg cccgatggtg gcggtgaatg cgggcgatcg
    48361 ttttgaagcc catattgaag gcataggttc agttgctgcg acattttcaa gcgcagcccc
    48421 aaaaggaagt ctgtcatgag taagcgtaaa gtcgccatta tcggttctgg caacattggc
    48481 accgatctga tgattaaaat tttgcgtcac ggtcagcatc tggagatggc ggtgatggtc
    48541 ggcattgatc ctcagtccga cggtctggcg agtgccagac gtatgggcgt cgccaccacc
    48601 catgaagggg tgatcgggct gatgaacatg cctgaatttg ctgatatcga cattgtattt
    48661 gatgcgacca gcgcgggcgc gcatgtgaaa aacgatgccg ctttacgcga agcgaaaccg
    48721 gatattcgct taattgacct gacgcctgct gccatcggcc cttactgcgt gccggtggtt
    48781 aacctcgagg cgaacgtcga tcaactgaac gtcaacatgg tcacctgcgg cggccaggcc
    48841 accattccaa tggtggcggc agtttcacgc gtggcgcgtg ttcattacgc cgaaattatc
    48901 gcttctatcg ccagtaaatc tgccggacct ggcacgcgtg ccaatatcga tgaatttacg
    48961 gaaaccactt cccgagccat tgaagtggtg ggcggcgcgg caaaagggaa ggcgattatt
    49021 gtgcttaacc cagcagagcc accgttgatg atgcgtgaca cggtgtatgt attgagcgac
    49081 gaagcttcac aagatgatat cgaagcctca atcaatgaaa tggctgaggc ggtgcaggct
    49141 tacgtaccgg gttatcgcct gaaacagcgc gtgcagtttg aagttatccc gcaggataaa
    49201 ccggtcaatt taccgggcgt ggggcaattc tccggactga aaacagcggt ctggctggaa
    49261 gtcgaaggcg cagcgcatta tctgcctgcc tatgcgggca acctcgacat tatgacttcc
    49321 agtgcgctgg cgacagcgga aaaaatggcc cagtcactgg cgcgcaaggc aggagaagcg
    49381 gcatgaacgg taaaaaactt tatatctcgg acgtcacgtt gcgtgacggt atgcacgcca
    49441 ttcgtcatca gtattcgctg gaaaacgttc gccagattgc caaagcactg gacgatgccc
    49501 gcgtggattc gattgaagtg gcccatggcg acggtttgca gggttccagc tttaactatg
    49561 gtttcggcgc acatagcgac cttgaatgga ttgaagcagc ggcggatgtg gtgaagcacg
    49621 ccaaaatcgc gacgttgttg ctgccaggaa tcggcactat tcacgatctg aaaaatgcct
    49681 ggcaggctgg cgcgcgggtg gttcgtgtgg caacgcactg taccgaagct gatgtttccg
    49741 cccagcatat tcagtatgcc cgcgagctcg gaatggacac cgttggtttt ctgatgatga
    49801 gccatatgac cacgccggag aatctcgcaa agcaggcaaa gctgatggaa ggctacggtg
    49861 cgacctgtat ttatgtggtg gattctggcg gtgcgatgaa catgagcgat atccgtgacc
    49921 gtttccgcgc cctgaaagca gagctgaaac cagaaacgca aactggcatg cacgctcacc
    49981 ataacctgag tcttggcgtg gcgaactcta tcgcggcggt ggaagagggc tgcgaccgaa
    50041 tcgacgccag cctcgcggga atgggcgcgg gcgcaggtaa cgcgccgctg gaagtattta
    50101 ttgccgccgc ggataaactg ggctggcagc atgggaccga tctctatgcg ttaatggatg
    50161 ccgctgacga cctggtgcgt ccgttgcagg atcgaccggt acgagtcgat cgcgaaacgc
    50221 tggcgctggg atacgctggt gtttactcga gcttcctgcg tcactgtgaa acggcggcgg
    50281 cgcgttatgg cttaagtgcg gtggatattc tcgttgagct gggcaaacgc cggatggttg
    50341 gcggccagga ggatatgatc gttgacgtgg cgctggatct gcgcaacaac aaataatgat
    50401 gactgccgag aacgtgcatt ttgtatgccc atcccagcac gactgccgga tgcggcgtaa
    50461 acaccttatc cggcctacgg gtggcgtgag aatttgtagg cctgataaga cgcgttagcg
    50521 tcgcatcagg catctgcaca cgactgccgg atgcgataaa cgtcttgtcc ggcctacatt
    50581 tcgcccgtag gcagtcatta aatagttctg attacgcggc tggcaatgta tcagtcgcga
    50641 ttcacctgca ctcgcaacga ggttcatctt atgtcgactc gtaccccttc atcatcttca
    50701 tcccgcctga tgctgactat cgggctttgt tttttggtcg ctctgatgga agggctggat
    50761 cttcaggcgg ctggcattgc ggcgggtggc atcgcccagg ctttcgcact cgataaaatg
    50821 caaatgggct ggatattcag cgccggaata ctcggtttgc tacccggcgc gttggttggc
    50881 ggaatgctgg cggaccgtta tggtcgcaag cgcattttga ttggctcagt tgcgctgttt
    50941 ggtttgttct cgctggcaac ggcgattgcc tgggatttcc cctcactggt ctttgcgcgg
    51001 ctgatgaccg gtgtcgggct gggggcggcg ttgccgaatc ttatcgccct gacgtctgaa
    51061 gccgcgggtc cacgttttcg tgggacagca gtgagcctga tgtattgcgg tgttcccatt
    51121 ggcgcggcgc tggcggcgac actgggtttc gcgggggcaa acttagcatg gcaaacggtg
    51181 ttttgggtag gtggtgtggt gccgttgatt ctggtgccgc tattaatgcg ctggctgccg
    51241 gagtcggcgg ttttcgctgg cgataaacag gctgcgccac cactgcgtgc cttatttgcg
    51301 ccagaaacgg caaccgcgac gctgctgctg tggttgtgtt atttcttcac tctgctggtg
    51361 gtctacatgt tgatcaactg gctaccgctg cttttggtgg agcaaggatt ccagccatcg
    51421 caggcggcag gggtgatgtt tgccctgcaa atgggtgcgg caagcgggac gttaatgttg
    51481 ggcgcattga tggataagct gcgtccagtg accatgtcgc tactgattta tagcggcatg
    51541 ttagcttcgc tgctggcact tggaacggtg tcgtcattta acggtatgtt gctggcggga
    51601 tttgtcgcgg ggttgtttgc gacaggtggg caaagcgttt tgtatgccct ggcaccgttg
    51661 ttttacagtt cgcagatccg cgcaacaggt gtgggaacag ccgtggcggt agggcgtctg
    51721 ggggctatga gcggtccgtt actggccggg aaaatgctgg cattaggcac tggcacggtc
    51781 ggcgtaatgg ccgcttctgc accgggtatt cttgttgctg ggttggcggt gtttattttg
    51841 atgagtcgga gatcaagaat gcagccgtgt gccgatgcct gacgcgcctt attaagcagg
    51901 ggcgaaaggg agttgccccc aatacaccca cggcgtttac aggtatactc gctaaaaatt
    51961 attcagcggg tttggaaaca aagatggcaa aacttacctt acaagagcag ttgctcaaag
    52021 caggattagt caccagcaaa aaagcggcga aggtggagag aacggcgaaa aaatcgcgcg
    52081 ttcaggcgcg tgaagctcgg gcggcggtag aagaaaataa aaaggcacag cttgagcgtg
    52141 ataaacagct tagcgagcaa caaaaacaag ctgcgttggc gaaagaatat aaagctcagg
    52201 tgaagcagct cattgaaatg aaccgaatca ccattgccaa tggcgatatt ggttttaact
    52261 tcactgacgg caatctgatt aagaagattt ttgtcgataa gctcacacag gcgcagttga
    52321 ttaatggtcg tctggcgatt gctcgcttgt tggtcgataa caatagcgaa ggtgaatacg
    52381 ccattattcc cgccagcgtt gccgataaaa tagctcagcg tgatgccagc agtatagtgt
    52441 tgcacagcgc gctcagcgcc gaagagcagg atgaagacga cccatatgcc gacttcaaag
    52501 tgcctgatga tttgatgtgg taaacataga taagtcgtgc gcagatgcct gatgcgacgc
    52561 tatgcgcgtc ttatcatgcc tacggcctgc gggcgaaatg taggcggata aggcgttcac
    52621 gccgcatccg gcagtcgtgc actattatca acgcatattc agtttattgg cgtggtaggc
    52681 aatatgctcg ccaataaaac tggagacaaa ataatagctg tggtcgtaac cctcgtgata
    52741 acggattaac gtcttgatat tcatctcctg gcagatcttt tcaagatttg gagtccgcag
    52801 ctgttccgcg taaaaatcat cactcaaccc ctgatcaacc atgatttccg caacgcgttg
    52861 accttgtgaa ataagactca ccggatcgta attcaaccag gcatctttat tttcaccaag
    52921 atatgcggca aaggcttgct gtccccacgg tacttgcgat ggggagacaa tgggcgaaaa
    52981 cgccgagacg ctgacatatt catctgggtt acgtaacgcc agcaccagcg cgcccagccc
    53041 gcccatagaa tgaccagaga tagactttct ggtcgttgcc ggaaaatgtt gcatcactaa
    53101 atccggcagc tcgttgcgga tatagtcata cattttgtaa tgttcattcc acggcgcttg
    53161 cgttgcgttc aggtaaaacc cggcaccttg cccgagatcg taacggtcgg catctgcgac
    53221 atgactgcct cgcggactgg tgtccggcgc aacaacaata atgttgtgtt cagccgcgta
    53281 acgctgcatc cccgatttag taatgaaatt ctgctcgttg caggtcaggc ccgaaagcca
    53341 gtacagcacc ggcaattttt cattcgcggc ttttggtggg agatagacgc cgacattcat
    53401 ttcacatttc agtgattggg aataatgccg atacacattt tgccagccgc caaagctggc
    53461 atgtttttca atgagttcca tcagtcatta tctcacggtt aatcccccgg acggctgccc
    53521 agggagagta ttaagaaccc cgtctgaagt ggacggggta tacctgcggg aaatcagtaa
    53581 cgaattacgg ttcgaatgga tttgccttca tgcatcaggt cgaaggcgtc attaatttca
    53641 tcaaggctca tggtatgcgt gacaaacggt tccagatcga tatcaccttt catcgcatct
    53701 tcaaccatac ccggtaactg ggaacgacct ttcacgccgc caaacgcgga acctttccat
    53761 acgcgaccag tgaccaactg gaatggacgg gtggagattt cctgaccaga acctgccacc
    53821 ccgatgatca ccgactgacc ccagccgcgg tgcgcacttt ccagcgccgc acgcatcacg
    53881 ttgacgttac cgatgcattc aaaggtatgg tcgatacccc atttgttgat atccagcagg
    53941 acatctttaa tcggtttgtc gtagtcattc gggttaatgc agtcggtagc accgaagcga
    54001 cgcgccagat cgaatttctt cgggttggta tcgatagcga taatccgtcc cgctttcgcc
    54061 tgacgcgcgc cctgaaccac tgccagacca atcgcgccaa gaccaaacac ggcaacagaa
    54121 tcacctggct ggactttagc tgtgttgtgc accgcgccaa taccggtggt cacgccacag
    54181 cccagcagac agacgtgttc atggtttgct tctggattaa ttttggccag agacacttcc
    54241 gcgaccacgg tgtattcact gaatgtagag caccccatgt agtgataaag cggctgcccg
    54301 ttgtaagaaa aacgggtggt gccgtctggc atcaagcctt taccctgggt ttcgcgaacc
    54361 gcaacacaga ggttagtttt gccagaacga cagaactcac actcgccgca ctccgcggtg
    54421 taaagcggga tcacatggtc gccaggtttg acgctggtta cgccttcacc gacttcaacc
    54481 acaacgccgg ccccttcgtg accgagaacc accgggaata caccttccgg gtcatccccg
    54541 gagagggtaa atgcgtcggt atggcaaacg ccggtatggg tgactttaat cagcacttca
    54601 ccttttttcg gtggtgcaac gtcaatttca acgatttcca gcggtttacc gggagcaaat
    54661 gcaacggcag cacgtgattt catctctcgc tcttcctcaa tatggtaata gattcagcta
    54721 tttaagatag gctcgaacca gttcaatagt gtcgtcaacg gattggctga cttcgcggct
    54781 gtagcagtca tttcggtcaa acgtttcccg gatatggctt tcaagcactt ctgccatcag
    54841 cccgttagcc gcgccccgaa cggcagcgat ctgttggagt atggcacggc attcggcatc
    54901 accctccatc gaccgttcca gagcatcaat ctgccctcga atacgacgaa ctcgagtaag
    54961 gacctttttc ttctcttccg gagtactggg catttcgcac ctcatcatct gcacgcaata
    55021 tactataggg gggtatacta catgtcaatg catacccccc tatagtatat aacactggag
    55081 aataaaattt atccggtgaa tgtggtcgga aaacaaagag gaaagggggg ggctaatcgg
    55141 cagggaaggc cgccccggat agcgggcggc agaaggaatc agaatttcca ggtcagatgg
    55201 gctgcaagtt gcagaccgtt ataatcatcg gttggggtgt cgtaccacac tttacctgcc
    55261 gtcagcccga gattaagttg ctcagtcagc ggttgaatac gttgcagact gacgctcttc
    55321 atgctgccgt agcgtgtttc tggcgtccag tcataggtgt aagcgcctgt tccgcggctt
    55381 agccatagtt gcgtataacc agtgccgcgc gggtcattca ggcgcacgga aatcatattg
    55441 ctataactac cacctgcatc gctggagtca taatgggtat agcggtagct ggtgatcacc
    55501 gggccagtat agagtgatac gccgccttgc caggcatcga cttcgacatc atcgtaatat
    55561 ttagtgtagc gataaccggt cgtgaaaagc gtttttggca acagggccag gttaatatcc
    55621 tgacgaaaat tctggcgggc aaagaccggc gtattatccg caaaggcaat acccgttcgg
    55681 gttgtcagcc agttattcca tttataccag accgcaccct gaccgcgagt ggcattgaaa
    55741 tgttcagttt cataatcacg acgcccctgg gaaagattaa aaagcagcgt tgcgttttca
    55801 actttcgcca ccagttcagc atacgctaaa ttacggttgc catgatcgcc agaataatcg
    55861 gtaaaatcgt agcccgcggt aatggaagtt aacccggcat aagcgggcaa tgcgctaaaa
    55921 atggccgccg ctaataacgt ccgttttatc attttaatct cctgtacgga taagttcttg
    55981 tcggagtgaa taagttccgg caggtaaatt acgttctaca atcacgcgcg ggccaagttg
    56041 cgtgttcggg gaaataattc gcccaggcaa aattataact tgtacgccaa ggcgtgaacg
    56101 ctgcccgata taacaaccta atttatcgca tccggtagcg ataattccct ctggagttcg
    56161 aacagacaca ggctgttcat ccagacgatg attactggta cgtacttgtg cgcccaaata
    56221 tgcctggttt gcaactaccg agtcggcaat aaaacattgc ggaccaatcg ttgcttccgc
    56281 ttcaataacc gcatttttaa tttcggtggc aaaaccaatt tttacaccat tgctgattat
    56341 tgtgccagga cgaataaacg cataattacc tatcaggcag tttgcgccaa tcactaccgg
    56401 accctgaata acggcaccat gacaaatacg ggtattcgcg ccgataacaa cttcacctgc
    56461 agtttcatca ataattacct ggtctgcaat ctgaatattt ttctctttaa cattaagctt
    56521 cagatagttt ttcagttcct gtgacagaag gttgggaata agcggttgac gcttccacca
    56581 gtgcggaata gcgggtgggt ttgtcgcgct gaggttcgcg tccagtaaaa aaggcaataa
    56641 gtccataaat aatccagatg gcataagcta ataatacata cacaacggaa agcggcgcta
    56701 aaggcaccaa caaccagcaa cgatgaaacc aggcgctaaa agcaccaata acacaaacta
    56761 cgccgatcca gataagcgga aacattgcca acaccacacc atgcggcccg gtggtgatga
    56821 attcattaaa ccaggtagtg agataaatcc caacgccata aagcacaacc aacagcatag
    56881 gaaatatact gaagataccg aagcggctaa ataaaagtct tttatgcagg cgcatacaga
    56941 ccgcgtatcc cacaatccaa cgccgccagc gacgccactc ctcacgcggg ctgttgcatt
    57001 cctgtgggta tacgatgcag cgattcgcct gccgaatacg gtagccgttt gccactaatg
    57061 tccaggtgag atcaaggtct tcgactttag tacgatccga gaaaccgaac ttacgcaata
    57121 catcagtacg gaacattccg caggcaccgc tgataataaa cggtgcgccg cccaggagct
    57181 gctgtagcgt gcgcttcata acaatcatcg gcaactttac ggtcgcgcgg atgtgcggta
    57241 acagccccgc gcctttcaac gcagtagagg gaatgccgcc tacggcatca gcaccgcgct
    57301 caatttctgc cagcatatag cccattccgt cttgatcggg cggaacatag gtgtcggcat
    57361 cacttaaaaa aacctggtcg caggtggcat aattgaggcc attcatcagc gcaccacctt
    57421 taccggtatt tttttgcgtg acggcaacaa agcggtcgcc ccatttgcgt ttgacttccg
    57481 ccatgaccgc ttcggtattg tccgtggagc cgtcgttaac gcaaattacc cggcaaaaat
    57541 aagggttacg cagtagatta tccagtgact gcgccagaca cgggccttcg ttatacgcag
    57601 gtataatggc gtcaatacag cctttctttt gactgggttt acgtcttagc gtacttaaaa
    57661 accatagcaa cattgctata gacatacaga taaatatcca ggttttcatg caatttgctc
    57721 aaagaatcat tttatgaatt acaaagcctt cacccagatc gctattgacc tgttgcccgc
    57781 gaaattgtgc aactgcacgc aggcgatcat gacgcatata atcacgtcgt tcctggcttt
    57841 tatgtttttt taatgcagca agtttgactg tgaaatattc ttctttaacg gattcaaaaa
    57901 cctgaggcat aaatgaaagc caggtacttg gggtttcgta gccgagaatt tgtggaatag
    57961 tgcggcaggc aaccattgaa gcttgataaa cagcgagatg atcctgatgg cggtcggcat
    58021 catgcatggt atatacccgc atgatttcaa catcagaagg aatttgattt ttaatgatgt
    58081 cttccagagc ggaaatcata tcattgagct gtaaatgagc gcgggtgtca gcaaaattaa
    58141 gatgaatagt ttggtggcac cctaatatct ttagggcgtt gcgcgattct tcatggcgat
    58201 cgattattcc atctgtacca gagttgccgg tagtcatcac cacggctgcg atataaattc
    58261 ctttttgcgc aagacgagca agcgatgcgc cacagcctaa ttctatatcg tcaggatgtg
    58321 cgccaatagc aaggataccc tttcttttat ttgccgaaga agggagggct gaatctaaaa
    58381 ccttatccac ttaatgacac tccattttat ttattatact acaagcacaa cgatgcactc
    58441 agagacgtaa tctctggcgc agccagatgt taatttataa atgttaaatg ttgctaataa
    58501 ctgaaaagca agggctttca gacccggagg ttcgactggg taatgttttt atgcaattca
    58561 tatgttaagt gtttgtatgt tttgtatgta tagttatttt gt
//
LOCUS       DAZFKM010000013        58560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFKM010000013 DAZFKM010000000
VERSION     DAZFKM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..476)
                     /gene="folK"
                     /locus_tag="ACHKAY_000903"
     CDS             complement(<1..476)
                     /gene="folK"
                     /locus_tag="ACHKAY_000903"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO5699620.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLAVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSK"
     gene            complement(473..1870)
                     /gene="pcnB"
                     /locus_tag="ACHKAY_000904"
     CDS             complement(473..1870)
                     /gene="pcnB"
                     /locus_tag="ACHKAY_000904"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO5699621.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1930..2826)
                     /gene="gluQRS"
                     /locus_tag="ACHKAY_000905"
     CDS             complement(1930..2826)
                     /gene="gluQRS"
                     /locus_tag="ACHKAY_000905"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO5699622.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2893..3348)
                     /gene="dksA"
                     /locus_tag="ACHKAY_000906"
     CDS             complement(2893..3348)
                     /gene="dksA"
                     /locus_tag="ACHKAY_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO5699623.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3526..4230)
                     /gene="sfsA"
                     /locus_tag="ACHKAY_000907"
     CDS             complement(3526..4230)
                     /gene="sfsA"
                     /locus_tag="ACHKAY_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO5699624.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4245..4775)
                     /gene="thpR"
                     /locus_tag="ACHKAY_000908"
     CDS             complement(4245..4775)
                     /gene="thpR"
                     /locus_tag="ACHKAY_000908"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO5699625.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4849..7278
                     /gene="hrpB"
                     /locus_tag="ACHKAY_000909"
     CDS             4849..7278
                     /gene="hrpB"
                     /locus_tag="ACHKAY_000909"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO5699626.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLSARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7410..9944
                     /gene="mrcB"
                     /locus_tag="ACHKAY_000910"
     CDS             7410..9944
                     /gene="mrcB"
                     /locus_tag="ACHKAY_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO5699627.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10164..12407
                     /gene="fhuA"
                     /locus_tag="ACHKAY_000911"
     CDS             10164..12407
                     /gene="fhuA"
                     /locus_tag="ACHKAY_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO5699628.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TVAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12458..13255
                     /gene="fhuC"
                     /locus_tag="ACHKAY_000912"
     CDS             12458..13255
                     /gene="fhuC"
                     /locus_tag="ACHKAY_000912"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO5699629.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13255..14145
                     /gene="fhuD"
                     /locus_tag="ACHKAY_000913"
     CDS             13255..14145
                     /gene="fhuD"
                     /locus_tag="ACHKAY_000913"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO5699630.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14142..16124
                     /gene="fhuB"
                     /locus_tag="ACHKAY_000914"
     CDS             14142..16124
                     /gene="fhuB"
                     /locus_tag="ACHKAY_000914"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO5699631.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16159..17439)
                     /gene="hemL"
                     /locus_tag="ACHKAY_000915"
     CDS             complement(16159..17439)
                     /gene="hemL"
                     /locus_tag="ACHKAY_000915"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO5699632.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17605..17652
                     /gene="yadX"
                     /locus_tag="ACHKAY_000916"
     CDS             17605..17652
                     /gene="yadX"
                     /locus_tag="ACHKAY_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO5699633.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17664..19085
                     /gene="clcA"
                     /locus_tag="ACHKAY_000917"
     CDS             17664..19085
                     /gene="clcA"
                     /locus_tag="ACHKAY_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO5699634.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19109..19174
                     /gene="yadW"
                     /locus_tag="ACHKAY_000918"
     CDS             19109..19174
                     /gene="yadW"
                     /locus_tag="ACHKAY_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO5699635.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19167..19511
                     /gene="erpA"
                     /locus_tag="ACHKAY_000919"
     CDS             19167..19511
                     /gene="erpA"
                     /locus_tag="ACHKAY_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO5699636.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19558..20181)
                     /gene="yadS"
                     /locus_tag="ACHKAY_000920"
     CDS             complement(19558..20181)
                     /gene="yadS"
                     /locus_tag="ACHKAY_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO5699637.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20219..21019)
                     /gene="btuF"
                     /locus_tag="ACHKAY_000921"
     CDS             complement(20219..21019)
                     /gene="btuF"
                     /locus_tag="ACHKAY_000921"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO5699638.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21012..21710)
                     /gene="mtnN"
                     /locus_tag="ACHKAY_000922"
     CDS             complement(21012..21710)
                     /gene="mtnN"
                     /locus_tag="ACHKAY_000922"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO5699639.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21794..23311
                     /gene="dgt"
                     /locus_tag="ACHKAY_000923"
     CDS             21794..23311
                     /gene="dgt"
                     /locus_tag="ACHKAY_000923"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO5699640.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23441..24865
                     /gene="degP"
                     /locus_tag="ACHKAY_000924"
     CDS             23441..24865
                     /gene="degP"
                     /locus_tag="ACHKAY_000924"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO5699641.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25022..26173
                     /gene="cdaR"
                     /locus_tag="ACHKAY_000925"
                     /pseudo
     CDS             25022..26173
                     /gene="cdaR"
                     /locus_tag="ACHKAY_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26262..26648)
                     /gene="yaeH"
                     /locus_tag="ACHKAY_000926"
     CDS             complement(26262..26648)
                     /gene="yaeH"
                     /locus_tag="ACHKAY_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO5699642.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26810..27622)
                     /gene="yaeI"
                     /locus_tag="ACHKAY_000927"
     CDS             complement(26810..27622)
                     /gene="yaeI"
                     /locus_tag="ACHKAY_000927"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO5699643.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27677..28501)
                     /gene="dapD"
                     /locus_tag="ACHKAY_000928"
     CDS             complement(27677..28501)
                     /gene="dapD"
                     /locus_tag="ACHKAY_000928"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO5699644.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28532..31204)
                     /gene="glnD"
                     /locus_tag="ACHKAY_000929"
     CDS             complement(28532..31204)
                     /gene="glnD"
                     /locus_tag="ACHKAY_000929"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO5699645.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31266..32060)
                     /gene="map"
                     /locus_tag="ACHKAY_000930"
     CDS             complement(31266..32060)
                     /gene="map"
                     /locus_tag="ACHKAY_000930"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO5699646.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32428..33153
                     /gene="rpsB"
                     /locus_tag="ACHKAY_000931"
     CDS             32428..33153
                     /gene="rpsB"
                     /locus_tag="ACHKAY_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO5699647.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33411..34262
                     /gene="tsf"
                     /locus_tag="ACHKAY_000932"
     CDS             33411..34262
                     /gene="tsf"
                     /locus_tag="ACHKAY_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO5699648.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34409..35134
                     /gene="pyrH"
                     /locus_tag="ACHKAY_000933"
     CDS             34409..35134
                     /gene="pyrH"
                     /locus_tag="ACHKAY_000933"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO5699649.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35426..35983
                     /gene="frr"
                     /locus_tag="ACHKAY_000934"
     CDS             35426..35983
                     /gene="frr"
                     /locus_tag="ACHKAY_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO5699650.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36075..37271
                     /gene="ispC"
                     /locus_tag="ACHKAY_000935"
     CDS             36075..37271
                     /gene="ispC"
                     /locus_tag="ACHKAY_000935"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO5699651.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37460..38218
                     /gene="ispU"
                     /locus_tag="ACHKAY_000936"
                     /gene_synonym="uppS"
     CDS             37460..38218
                     /gene="ispU"
                     /locus_tag="ACHKAY_000936"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO5699652.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38231..39088
                     /gene="cdsA"
                     /locus_tag="ACHKAY_000937"
     CDS             38231..39088
                     /gene="cdsA"
                     /locus_tag="ACHKAY_000937"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5699653.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39100..40452
                     /gene="rseP"
                     /locus_tag="ACHKAY_000938"
     CDS             39100..40452
                     /gene="rseP"
                     /locus_tag="ACHKAY_000938"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO5699654.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40482..42914
                     /gene="bamA"
                     /locus_tag="ACHKAY_000939"
     CDS             40482..42914
                     /gene="bamA"
                     /locus_tag="ACHKAY_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO5699655.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43036..43521
                     /gene="skp"
                     /locus_tag="ACHKAY_000940"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43036..43521
                     /gene="skp"
                     /locus_tag="ACHKAY_000940"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO5699656.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43525..44550
                     /gene="lpxD"
                     /locus_tag="ACHKAY_000941"
     CDS             43525..44550
                     /gene="lpxD"
                     /locus_tag="ACHKAY_000941"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5699657.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44655..45110
                     /gene="fabZ"
                     /locus_tag="ACHKAY_000942"
     CDS             44655..45110
                     /gene="fabZ"
                     /locus_tag="ACHKAY_000942"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO5699658.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45114..45902
                     /gene="lpxA"
                     /locus_tag="ACHKAY_000943"
     CDS             45114..45902
                     /gene="lpxA"
                     /locus_tag="ACHKAY_000943"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO5699659.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45902..47050
                     /gene="lpxB"
                     /locus_tag="ACHKAY_000944"
     CDS             45902..47050
                     /gene="lpxB"
                     /locus_tag="ACHKAY_000944"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO5699660.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47047..47643
                     /gene="rnhB"
                     /locus_tag="ACHKAY_000945"
     CDS             47047..47643
                     /gene="rnhB"
                     /locus_tag="ACHKAY_000945"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO5699661.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47680..51162
                     /gene="dnaE"
                     /locus_tag="ACHKAY_000946"
     CDS             47680..51162
                     /gene="dnaE"
                     /locus_tag="ACHKAY_000946"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO5699662.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51175..52134
                     /gene="accA"
                     /locus_tag="ACHKAY_000947"
     CDS             51175..52134
                     /gene="accA"
                     /locus_tag="ACHKAY_000947"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO5699663.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52233..54374
                     /gene="ldcC"
                     /locus_tag="ACHKAY_000948"
     CDS             52233..54374
                     /gene="ldcC"
                     /locus_tag="ACHKAY_000948"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO5699664.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54431..54820
                     /gene="yaeR"
                     /locus_tag="ACHKAY_000949"
     CDS             54431..54820
                     /gene="yaeR"
                     /locus_tag="ACHKAY_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5699665.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54885..56183
                     /gene="tilS"
                     /locus_tag="ACHKAY_000950"
     CDS             54885..56183
                     /gene="tilS"
                     /locus_tag="ACHKAY_000950"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO5699666.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56232..56492)
                     /gene="rof"
                     /locus_tag="ACHKAY_000951"
     CDS             complement(56232..56492)
                     /gene="rof"
                     /locus_tag="ACHKAY_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO5699667.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56479..56679)
                     /gene="yaeP"
                     /locus_tag="ACHKAY_000952"
     CDS             complement(56479..56679)
                     /gene="yaeP"
                     /locus_tag="ACHKAY_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO5699668.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56845..57390
                     /gene="yaeQ"
                     /locus_tag="ACHKAY_000953"
     CDS             56845..57390
                     /gene="yaeQ"
                     /locus_tag="ACHKAY_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO5699669.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSDFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57387..57809
                     /gene="arfB"
                     /locus_tag="ACHKAY_000954"
     CDS             57387..57809
                     /gene="arfB"
                     /locus_tag="ACHKAY_000954"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO5699670.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57823..58533
                     /gene="nlpE"
                     /locus_tag="ACHKAY_000955"
                     /gene_synonym="cutF"
     CDS             57823..58533
                     /gene="nlpE"
                     /locus_tag="ACHKAY_000955"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO5699671.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13743 a        14542 c        16309 g        13966 t
ORIGIN      
        1 catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt ctccccatca
       61 ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc acgattcttc
      121 atatcgtagt gcggaacggc caggcgttcg gtatttatca cttcattacc aaacagcatg
      181 atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg accctgttgc
      241 agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgagcgaagt ttcaagcgcc
      301 acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt gcggtaaaac
      361 gaagaaacgg caagaatgtg gctttcaggg atatcgccta atgctttcag ggcagcattg
      421 acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac tgtcatgcgg
      481 taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acggcgacgc ggtgacggct
      541 cttcatccaa ctcgttaagc atcccttttt gatctggtgg cgcggaaacc tggaactcac
      601 cccaccattt caccagacgc tgcagttcag cgttacgttc aacttcagct cgcaaggcca
      661 acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg cgtttaccct
      721 gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc gtcagacgtt
      781 tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc agcgcgaaag
      841 cgtcgtgata ggtcaggccg ctttcctggg caatcttctg tgccgtctcc agcagtgggt
      901 accagaacat ggcggcaaac aggaacgccg ggttcacgcg catatcgtta tggatacgcg
      961 tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg ccattttccg
     1021 tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca cacaacagct
     1081 tataggtatc gtaaccgtag cccgcttgta gcagtttaag cgattcttca aacaggcgtg
     1141 ccggtgggat atcgttcaac agggtagcga ggcgagggat cggttctgcg gtttccgggc
     1201 tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt accggatctt
     1261 cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc agatccttca
     1321 taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatagagg ctgttgatag
     1381 tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca
     1441 tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg tggtgtccac
     1501 ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga cggaaacggc
     1561 gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg gcgttagtgg
     1621 ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca
     1681 gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca ttttcactga
     1741 tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt ggacgggcga
     1801 ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc
     1861 gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa aatagcggct
     1921 aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg actcgggcac
     1981 ggcggtaagc cgccagtttt tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg
     2041 ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta ccgggcgtgg
     2101 atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt
     2161 aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct ggtacagcga
     2221 gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg taacgccctg
     2281 gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac ggcgatgaat
     2341 gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgccgcgca gctggtcagt
     2401 aaattgcgtg accggatgct gctggcggat acgcactgcg gcattgtctg gtccatgatg
     2461 caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg cacgcgtaca
     2521 ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac gataggcgtg
     2581 gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat gttccagctg
     2641 gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat cttctatgcg
     2701 taccagccag cgaccttgcc gggcgcgagc ctgcaaatag ctgccgagcg cggcgatcag
     2761 agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat actgtgtgtc
     2821 tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg
     2881 gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc
     2941 gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt caacaccgca
     3001 ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct cgatcttttt
     3061 gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt cttcctgggc
     3121 tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg
     3181 atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga agtgcgccag
     3241 ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca ccccagcgat
     3301 ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt tgcttctcct
     3361 taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata aatagcagat
     3421 gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag gaaaagcgta
     3481 tttgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca atgtaaccgg
     3541 cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag ccagaatttc
     3601 tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc
     3661 gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg cacgctggcc
     3721 ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag tgaccgcatc
     3781 gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc
     3841 tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttctgcgc cgtattttac
     3901 ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa ggatagcctc
     3961 tttcgtcaac ctgttagccc aaagcgtgtt gacgcaaata aatgcgccgc tctggctttg
     4021 agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg aataccagac
     4081 ggtatcgcca ggcgttgcac aaccggtcat tgcacccgta ttcgggcagt gtagcgttaa
     4141 ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt
     4201 cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg cgttagcgcc
     4261 cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg
     4321 aactccgtca ccgcatacga ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg
     4381 gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa acaaccgctg
     4441 cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg cggctgacgc
     4501 atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag cgtgagtgtg
     4561 aaaccaggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt ctcggcgctc
     4621 acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg acgtcccgcc
     4681 tcaggtggga agtgtgtggc gcgccagtgg ataatctgtt cgcggatttc tgcaggtaag
     4741 tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt atgaattacc
     4801 gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg
     4861 cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc gcaggtatta
     4921 ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact gctggcgcat
     4981 cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctgtcggc gcgtaacgtc
     5041 gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg ctaccggatg
     5101 cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tagttaccga aggcgtgctg
     5161 acgcggatga tccagcgtga cccggagttg agcggtgttg gattggtgat cctcgatgag
     5221 tttcatgagc gcagcttgca ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa
     5281 ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa cgaccgcttg
     5341 cagcaaatgc tgccagaagc gcccgtcgtc atctcagaag ggcgctcgtt tccggttgaa
     5401 cgccgttatt taccactgcc cacgcatcag cgttttgacg aagccgttgc ggtagccacc
     5461 gctgaaatgc tgcgtcagga aagtggatca ttactgttat ttttacctgg cgtcggagaa
     5521 attcagcgtg tgcaggaaca actggcttcg cgcatcggca gcgatgtatt gctctgcccg
     5581 ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga tcctcccggc accgcaaggg
     5641 atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat cgaaggtatt
     5701 cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg cacggggctt
     5761 acgcgactga ttactcaacg cgttagccag gcatccatga cgcagcgtgc cgggcgcgcc
     5821 gggcgtctgg agccgggtat ctgcctgcat ttaatcgcca aggaacaagc agaacgcgcc
     5881 gcggcgcaaa gtgaaccgga gatattacaa agcgatcttt ccggtttgct gatggaatta
     5941 ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc gccagtagtg
     6001 aatctactgg ccgcgaaacg tctgttacaa atgctggggg cactggaggg tgaacggctt
     6061 agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc ggcaatgctg
     6121 gttagcgcga agaacgacga cgaagctgct accgcggcaa aaattgccgc cattctcgaa
     6181 gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa tcaaccagcc
     6241 tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgtggcgg tgaggcagac
     6301 agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc tcgtcgccgt
     6361 gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga tgccaacgac
     6421 gcgctaagcc gccacgaatg gttgatcgca ccgttattat tgcagggcag cgcctcgccg
     6481 gatgcgcgga ttttactggc gctgccggtc gatattgatg agttagtaca acgctgcccg
     6541 cagctggtac agcagtctga cactgtggag tgggatgacg cgcaaggtac gctgaaagcc
     6601 tggcgtcggc tgcaaatcgg tctgctgacg gtgaaagtgc agccgctggc gaaaccctcg
     6661 gaagacgagt tgcatcaggc gatgcttaat ggcatccgcg ataaagattt aagcgtgctc
     6721 aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg
     6781 ccggaatatg actggccagc ggttgatgat gaaagtttat tggcgacgct ggaaacgtgg
     6841 ctgctgccac atatgactgg cgtacattca ctacgcggcc tgaaatcact cgacatttat
     6901 caggcactac gcggattact tgattgggga atgcagcaac gtctggatag tgaattgcct
     6961 gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga agataacccg
     7021 cccgcgctgg cggtgagaat gcaagagatg tttggcgagg ccaccaatcc gacgatcgcc
     7081 caggggcgcg tgccgctggt gctggagttg ctttcacctg cccaaaggcc attacaaatc
     7141 acacgagatt tgagcgcctt ctggaaagga gcgtaccgtg aggtgcaaaa agagatgaaa
     7201 gggcgttatc ccaaacatgt ctggccggac gacccggcaa atactgcacc gacgcgacgg
     7261 acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg ggaccgatcg
     7321 agcacaaatt ttgagagata tcttcttctg tcttgtaaca gaagaacaga aaatcgggct
     7381 tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag ccaattggac
     7441 gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg
     7501 acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg cgcaaaggta
     7561 agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg ctggctatgg ctgctgttaa
     7621 aactggctat cgtttttgcc gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa
     7681 ttcgtagccg tattgatggc aaggtctggc aactgcctgc ggcagtttat ggccgaatgg
     7741 tcaaccttga gccagatatg accatcagca agaacgagat ggtgaagctg ctggaggcga
     7801 ctcagtatcg tcaggtgtcg aaaatgaccc gtcctggcga atttaccgtg caggccaaca
     7861 gcattgagat gatccgccgt ccgtttgatt tcccggacag taaagaaggg caggtgcgtg
     7921 cgcgtctgac ctttgatggc gatcatctgg cgacgatcgt caatatggag aacaaccgtc
     7981 agttcggttt cttccgtctt gatccgcgtc tgatcaccat gatctcctcg ccaaacggtg
     8041 agcagcgttt gtttgtgccg cgcagtggtt tccctgattt gctggtggat actttgctgg
     8101 cgacagaaga ccgtcatttt tacgagcatg acggcatcag cctctactcc atcggacgtg
     8161 cggtgctggc aaacctgacc gccggacgca cggtgcaggg agcgagtacg ctgacgcaac
     8221 agctggtgaa aaacctgttc ctctccagtg agcgttctta ctggcgtaaa gcgaacgaag
     8281 cttacatggc gctgatcatg gacgcgcgtt acagcaaaga ccgtattctt gagctgtata
     8341 tgaacgaggt gtatctcggt cagagcggcg acaacgaaat ccgcggcttc ccgctggcga
     8401 gcttgtatta ctttggtcgc ccggtagaag agctaagcct cgaccagcag gcgctgttag
     8461 tcggcatggt gaaaggagcg tccatctaca acccgtggcg taacccaaaa ctggcgctgg
     8521 agcgacgtaa tctggtgctg cgtctgctgc aacagcaaca gattattgat caagaactct
     8581 atgacatgtt gagtgcacgt ccgctgggtg ttcagccacg cggtggggtg atctctccac
     8641 agccagcctt tatgcaactg gtgcgtcagg agctgcaggc gaaattaggc gataaggtaa
     8701 aagatctctc cggcgtgaag atcttcacta cctttgactc ggtggcccag gacgcggcag
     8761 aaaaagccgc cgtggaaggc attccggcac tgaagaaaca gcgtaagttg agcgatcttg
     8821 aaactgcgat tgtggtcgtc gaccgcttta gtggtgaagt tcgtgcgatg gtcggtggtt
     8881 ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc
     8941 ttgcaaaacc agcgacttat ctgactgcct taagccagcc gaaaatctat cgtctgaata
     9001 cgtggattgc ggatgcgcca atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc
     9061 agaatgatga ccgtcgttat agcgaaagcg gcagagtgat gctggtggat gcgttgaccc
     9121 gttcgatgaa cgtgccgacg gtaaatctgg ggatggcgct ggggctgcct gcggttacgg
     9181 agacctggat caaactgggc gtaccgaaag atcagttgca tccggttccg gcgatgctgc
     9241 tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc gttccagacc atcgccagcg
     9301 gcggtaaccg tgcaccgctt tctgcactgc gttcggtaat tgcggaagat ggcaaggtgc
     9361 tgtatcagag cttcccgcag gcggaacgcg ctgttccggc gcaggcggcg tatctgacac
     9421 tatggaccat gcagcaggtg gtacaacgcg gtacgggtcg tcagcttggg gcgaaatacc
     9481 cgaacctgca tctggcaggg aaaacaggga ctaccaacaa taacgtagat acctggtttg
     9541 caggcattga cggcagcacg gtgaccatca cctgggtagg ccgtgataac aaccagccga
     9601 ccaaactgta tggagccagc ggggcaatgt cgatttatca gcgttatctg gctaaccaga
     9661 cgccaacgcc gctgaatctt gttccgccag aagatattgc agatatgggc gtggactacg
     9721 acggcaactt tgtttgcagc ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc
     9781 aatcgctgtg ccagcagagc gagatgcagc agcagccgtc aggcaatccg tttgatcagt
     9841 cttctcagcc gcagcaacag ccgcaacagc aacctgctca gcaagagcag aaagacagcg
     9901 acggtgtagc cggttggatc aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat
     9961 accggatggc gagttgccat ccggtaaaat aacatcccat ctaagatatt aaccctttct
    10021 tttcatctgg ttgtttatta acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc
    10081 cgccagcgtt tcgaatatta tcttatcttt ataataatca ttctcgttta cgttatcatt
    10141 cactttacat cagagatata ccaatggcgc gttccaaaac tgctcagcca aaacactcac
    10201 tgcgtaaaat cgcagttgta gtagccacag cggtaagcgg catgtctgtt tatgcacagg
    10261 cagcggttga accgaaagaa gacactatca ccgttaccgt tgcacctgcg ccgcaagaaa
    10321 gcgcatgggg gcctgctgca actattgcgg cgcgacagtc tgctaccggc actaaaaccg
    10381 atacgccgat tcaaaaagtg ccacagtcta tttctgttgt gaccgccgaa gagatggcgc
    10441 tgcatcagcc gaagtcggta aaagaagcgc ttagctacac gccgggtgtc tctgttggta
    10501 cgcgtggcgc atccaacacc tatgaccacc tgatcattcg cggctttgcg gcagaaggcc
    10561 aaagccagaa taactatctg aatggcctga agttgcaggg caacttctat aacgatgcgg
    10621 tcattgaccc gtatatgctg gaacgcgctg aaattatgcg tggcccggtt tccgtgcttt
    10681 acggtaaaag cagtcctggc ggcctgttga atatggtcag caagcgtccg accaccgaac
    10741 cgctgaaaga agttcagttt aaagccggta ctgacagcct gttccagact ggttttgact
    10801 ttagcgattc gttggatgat gacggtgttt actcttatcg cctgaccggt cttgcgcgtt
    10861 ctgccaatgc ccagcagaaa gggtcagaag agcagcgtta tgctattgca ccggcgttca
    10921 cctggcgtcc ggatgataaa accaatttca ccttcctttc ttacttccag aacgagccgg
    10981 aaaccggtta ttacggctgg ttgccgaaag agggaaccgt tgagccgctg ccgaacggta
    11041 agcgtctgcc gacagacttt aatgaagggg cgaagaacaa cacctattct cgtaatgaga
    11101 agatggtcgg ctacagcttc gatcacgaat ttaacgacac ctttactgtg cgtcagaacc
    11161 tgcgctttgc tgaaaacaaa acctcgcaaa acagcgttta tggttacggc gtctgctccg
    11221 atccggcgaa tgcttacagc aaacagtgtg cggcattagc gccagcggat aaaggccatt
    11281 atctggcacg taaatacgtc gttgatgatg agaagctgca aaacttctcc gttgataccc
    11341 agttgcagag caagtttgcc actggcgata tcgaccacac cctgctgtcc ggtgtcgact
    11401 ttatgcgtat gcgtaatgac atcaacgcct ggtttggtta cgacgactct gtgccactgc
    11461 tcaatctgta caatccggtg aataccgatt tcgacttcaa tgccaaagat ccggcaaact
    11521 ccggccctta ccgcattctg aataaacaga aacaaacggg cgtttatgtt caggatcagg
    11581 cgcagtggga taaagtgctg gtcaccctag gcggtcgtta tgactgggca gatcaagaat
    11641 ctcttaaccg cgttgctggg acgaccgata aacgtgatga caaacagttt acctggcgtg
    11701 gtggtgttaa ctacctgttt gataatggtg taacacctta cttcagctat agcgaatcgt
    11761 ttgaaccttc ttcgcaagtt gggaaggatg gtaatatttt cgcaccgtct aaaggtaagc
    11821 agtatgaagt cggcgtgaaa tatgtaccgg aagatcgtcc gattgtagtt actggtgccg
    11881 tgtataatct cactaaaacc aacaacctga tggcggaccc tgagggttcc ttcttctcgg
    11941 ttgaaggtgg cgagatccgc gcacgtggcg tagaaatcga agcgaaagcg gcgctgtcgg
    12001 cgagtgttaa cgtagtcggt tcttatactt acaccgatgc ggaatacacc accgatacta
    12061 cctataaagg caatacgcct gcacaggtgc caaaacacat ggcttcgctg tgggctgact
    12121 ataccttctt tgacggtccg ctttcaggtc tgacgctggg caccggtggt cgttatactg
    12181 gctccagcta tggtgatccg gctaactcct ttaaagtggg aagttatacg gtcgtggatg
    12241 cgttagtgcg ttatgatctg gcgcgagtcg gcatggcggg ctccaacgtg gcgctgcatg
    12301 ttaacaacct gttcgatcgt gaatacgtcg ccagctgctt taacacttat ggctgcttct
    12361 ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt ctcttttggg
    12421 gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca cgaatcattc
    12481 cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca
    12541 tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc acaacggttc
    12601 tggtaaatcc actctgctca aaatgcttgg ccgtcatcag cctccgtcag aaggggagat
    12661 tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc
    12721 ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac tggtggcgat
    12781 tggtcgttac ccgtggcatg gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt
    12841 cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg tcgatagtct
    12901 ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg atagccgttg
    12961 tctgttgctc gacgaaccga cttcggcgct ggatatcgcc caccaggttg atgtgctggc
    13021 gctggtgcac cgtttaagtc aggaacgtgg cctgacggtc attgccgtgc tgcacgatat
    13081 caatatggcg gcacgctact gtgattatct ggtcgccctg cgcggtggtg aaatgattgc
    13141 tcagggaacg cctgcggaaa ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc
    13201 gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt attgatgagc
    13261 ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc tccgttgtta
    13321 tggcagatga ataccgccca cgcggcggct attgacccca atcgtattgt ggcgctggag
    13381 tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc
    13441 atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat tgacgtcggt
    13501 ttgcgtacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt tatggtctgg
    13561 tcggcaggat atggcccttc accagaaatg ctggcacgta ttgcgccggg tcgcggattt
    13621 aacttcagtg acggcaaaca gccgttggcg atggcgcgta aatcgctgac ggaaatggca
    13681 gatttactta acctgcaaag cgcagcagaa acgcatttag cgcactatga agactttatc
    13741 cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttactgct gacgacgctt
    13801 atcgatccgc gccatatgct ggtcttcggt ccaaacagct tgttccagga aattctcgat
    13861 gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag taccgccgtc
    13921 agtatcgatc gtctggcggc gtataaagac gttgatgtgc tctgttttga tcacgacaac
    13981 agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc gtttgtccgt
    14041 acagggcgct ttcagcgcgt acccgcagtc tggttttatg gtgcaacgct ttcggcaatg
    14101 cactttgtgc gtgttctgga taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc
    14161 ttttcccggc gttattgctg gcgctgttag tgattgtcgc tacggcgcta acctggatga
    14221 acttctcaca ggcgctgccg cgcagtcagt gggcgcaggc cgcctggtcg ccggatattg
    14281 acgtcatcga gcagatgatt tttcactaca gcttgttgcc gcgtctggcg atttcgctgc
    14341 tggtgggcgc aggcctgggg ctggtgggcg tgctgtttca gcaagtgctg cgtaatccgc
    14401 tggcggaacc gacgacactt ggcgttgcta caggcgcgca actgggaatt accgtcacca
    14461 cactttgggc gatcccaggt gctatggcga gccagtttgc cgcactggtg ggcgcttgtg
    14521 tcgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg gttacgctta
    14581 ttctcgcggg gctggtggtg agcctttatt gcggggcaat taatcagcta ctggttatct
    14641 tccatcacga ccagctgcaa agcatgttcc tgtggagcac cggaacgctg acgcaaaccg
    14701 actggggcgg cgttgagcgt ttatggccgc agttgctggg cggcgtgatg ttgacgttat
    14761 tgctacttcg cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc
    14821 tggccttgtc gctcgcgcgt ctggcagcgc tgtcgctggc gattgtcatc agtgcgctgc
    14881 tggtgaacgc tgtggggatt atcggcttta tcgggttgtt cgcgccgctg ctggcaaaaa
    14941 tgctgggggc gcggcgtctg ctgccacgac tgatgctggc gtcgctgatt ggggcgctga
    15001 tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg gaagtgtcca
    15061 ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgttg ccgcgtttac
    15121 gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcggctgaa cgccaacatg
    15181 tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg gtggcgctgt
    15241 cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgttgctt gaggatttga
    15301 tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggcgtcatg ctggcggtgg
    15361 cgggctgtat tattcagcgg ctgaccggaa acccgatggc aagcccggaa gtgctgggga
    15421 ttagctctgg cgcagcgttt ggcgtggtgt tgatgctgtt tctggtgccg ggtaatgcct
    15481 ttggctggct gttacctgca ggcagtctcg gcgcggcggt gacgctgttg atcattatga
    15541 tcgccgccgg ccgcggtgga ttttccccac accgcatgtt actggcgggg atggcgttaa
    15601 gcaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc
    15661 aagtgctgac ctggatttcc ggttcgacct acaacgcgac cgatgcgcag gtctggcgca
    15721 cgggaattgt gatggtgatt ttgctggcga ttaccccgct gtgccgccgc tggctgacca
    15781 ttttaccgct gggtggtgat accgcccgag ccgtaggaat ggcgctgacg ccgacgcgaa
    15841 ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgact attggaccgt
    15901 tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt cgacggacga
    15961 tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtgttc gctgactggt
    16021 gtgggcggat ggtgctgttt ccattccaga tcccagcagg gctgctgtcg acctttatcg
    16081 gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaatagaat gcctcgcccg
    16141 gtgggcgagg caaaatcatc acaacttcgc aaacacccgt cgtgcagcat cgatggtgtt
    16201 attgatatct tccatgctgt gcgccacgga cataaagccc gcttcaaacg ctgacggtgc
    16261 caggtaaaca ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt ccacgtcaca
    16321 ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa taccgaacat
    16381 gccgccaacg tggttaacga ccagcggaat tccggcttct tctgccgctt ccagcagacc
    16441 ttctgccaga cgtgttgtca gctcatccag cgtttcgtga acgcccggct gcgcgacttc
    16501 attcagacag gcgaaacccg ctgccatcgc aattgggtta ccggaaagcg tacccgcctg
    16561 atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac cgaatgcgcc
    16621 taccggcatt ccaccgccga tgattttgcc caggcaggtg agatccggtt ccacaccgta
    16681 ataatcctgt gcgccagcta gcgctacgcg gaagccagtc attacttcat cgatgatcag
    16741 caatgcgcca aattcgtcgc acagcgcgcg cagacctggc aggaactctg gcagcggtgg
    16801 aacgcagttc atattgcctg ccaccggctc gacgataata caggcaatct cttgcgggta
    16861 ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta aggtatgttt
    16921 ggcgaaatct gccggaacac ccggcgagtt tggctggcct aacgtgagtg cgccagaacc
    16981 ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt
    17041 gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atcgtcgctt cagtgccgga
    17101 gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt
    17161 cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt caatcacggc
    17221 attgcggata gccggatggt tatggcccag caccatcggc ccccaggaac cgacataatc
    17281 gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt cgataaacag
    17341 tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc cagggatcag
    17401 ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct gattcgtaga
    17461 aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg tcaaacatca
    17521 ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt gcatttgaaa
    17581 cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc atcttatttg
    17641 tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa acaccgcagg
    17701 ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat aaaaccccgt
    17761 tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggca gcggttgctt
    17821 ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta catactgctg
    17881 ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg
    17941 gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc ccggaaattg
    18001 aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg gtgaaattct
    18061 ttggcgggct ggggacactc ggcggaggca tggtgctagg gcgcgaagga ccaaccgtgc
    18121 agatcggcgg caatatcggt cgcatggtgc ttgatatttt ccgcctgaaa ggtgatggag
    18181 ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc tttaacgcgc
    18241 cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc tatacgttaa
    18301 tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac cggattttta
    18361 atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt aatacgctgt
    18421 ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt aataaatggg
    18481 tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc aaatgggtac
    18541 tgatgggtgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt
    18601 cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc atgggaatgc
    18661 tggtgtttat tttcgtcgct cgggtcatta ccaccttact ctgcttctct tccggcgcgc
    18721 cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc gctttcggaa
    18781 tggttgccgt tgagctgttt ccgcaatatc acctggaggc ggggacgttt gctattgccg
    18841 gaatgggggc attactggcg gcatctattc gcgcgccgtt aacggggatc attctggttc
    18901 tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt cttggcgcaa
    18961 cactattagc gcaatttacc ggcgggaaac cgctatactc ggcgattctt gcgcgcacgc
    19021 tggcaaaaca ggaagctatg caactggcgc gaagcaaggc cgcatcagcc agcgagaata
    19081 cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag aatttgccca
    19141 attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg ctggagttta
    19201 ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac ccgaatctga
    19261 aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt ttcacctttg
    19321 atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc ttggtggttg
    19381 atccgatgag cctgcaatat ctggtcggcg gttccgttga ctataccgaa ggactggaag
    19441 gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct
    19501 ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct tctctcttca
    19561 acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa tagccgccag
    19621 ccgaatcaag agcgtcacga ccatgcccat catactggct gtttccagtg gtacggaaaa
    19681 tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg cgtagatttc
    19741 tgtacgtaaa atcatgggga tttcgcgggc cagaacatca cgaatgatcc cgccgccaac
    19801 gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat taaaggcttt
    19861 attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg gcaacatcca
    19921 ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg ttaccattgc
    19981 aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca gcgccatgtc
    20041 gcgaattgtc ccgccgccta cagcggtaac cacgcccagt accagaacac caaaagggtc
    20101 catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg tgccgactat
    20161 atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag gtcaggcact
    20221 aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata cgtgggcttg
    20281 cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt tcaccccagt
    20341 attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc tgtggcgagc
    20401 gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct ttaaagatgt
    20461 tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca ctggtaaatg
    20521 gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca tattgcgctt
    20581 tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggccttg tctggttgcg
    20641 gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga
    20701 cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg gcattacctc
    20761 cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga ttcatcccct
    20821 gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa tagctgctga
    20881 ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga gaaagcgtga
    20941 tgacgcgcgg cgcggcgttg agccacagtg gcgcaagaaa agacagggcg accagcgccc
    21001 tgaacagtga cttagccatg tgcaagtttc tgcaccagtg actcaaccat caggctggac
    21061 tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg atcggccacg
    21121 tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac atgggcgatt
    21181 gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat tttcgccaga
    21241 ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac agcgttaaga
    21301 ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct
    21361 gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc cgcgtcgtga
    21421 taacgtgctt cgtccgagac aacgatatcg cccactttca acgttggtgc caggccaccg
    21481 gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcaa agtggcaccc
    21541 agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt tccattcagt
    21601 tggccggtat agatttcgca accgccgaga ctgatagttt gacgtttttc gattttgtca
    21661 cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat agatttactc
    21721 gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc gtatttctca
    21781 cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc atcgtcgtta
    21841 ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg agagcgatcg
    21901 cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagacgc aggtttttcc
    21961 actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag tccagcaggt
    22021 ggggcgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat tgctggaagc
    22081 atacggcctg gatgaactga ccggaccttt tgaaagcatt gttgagatgt catgcctgat
    22141 gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcagcgataa atgactggtt
    22201 tcgccaacgt ttgcacccgg aagatgccga aagccagcct ctgactgacg atcgctgcag
    22261 cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc ggcgcaagat
    22321 tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt
    22381 gatgcggatg aatctcacct gggcacaggt tggcggtatt ttaaaatata cccgtccggc
    22441 gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc cgggttatta
    22501 tctttctgaa gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg cgctttacag
    22561 tcgttttcca ttaacttgga ttatggaagc tgccgacgac atctcctatt gtgtggcaga
    22621 ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc atcatttgca
    22681 cgaagcgtgg ggccagcatg agaaaggttc gctcttttcg ctggtggttg aaaatgcctg
    22741 ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt
    22801 acgggtaaac accctaaata aactggtacc ctacgcggca caacgattta ttgataatct
    22861 gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag
    22921 cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc atccagatgt
    22981 cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgtcc
    23041 tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg
    23101 tttccctatt gaatcgcgct tattccacaa actctcgacg cgccatcggc tggcctatgt
    23161 cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta
    23221 ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg cgtgggatga
    23281 ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat
    23341 ttttaccttt tgcagaaact ttagttcgga acttcgcgct ataaaacgaa tctgaagaac
    23401 acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa ccacattagc
    23461 actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc
    23521 tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa
    23581 ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg
    23641 tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc aggaaggttc
    23701 accgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg gcggccagca
    23761 acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt
    23821 caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctgg gcgatggtcg
    23881 taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc tgattcagat
    23941 ccagaacccg aaaaacctga ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg
    24001 tgattacacc gtagcgattg gtaacccgtt tggcctggga gagacggtaa cttccgggat
    24061 tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac tacgaaaact tcatccagac
    24121 cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga acggcgaact
    24181 gatcggtatc aacaccgcga tcctcgcacc ggacggcggc aacatcggta tcggttttgc
    24241 tatcccgagc aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa
    24301 acgcggtgag ctgggtatta tggggactga gctgaattcc gaactggcga aagcgatgaa
    24361 agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa
    24421 agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccaa tcagcagctt
    24481 tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga ccctgggctt
    24541 gctgcgcgac ggtaagcagg tcaacgtgaa tctggaactg cagcagagca gccagaatca
    24601 ggttgattcc agctccatct tcaacggcat tgaaggcgct gagatgagca acaaaggcaa
    24661 agatcagggc gtggtagtga acaacgtgaa aacgggcact ccggctgcgc agatcggcct
    24721 gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca tcgctgaact
    24781 gcgtaaagtt cttgacagca aaccgtctgt actggcactg aacattcagc gcggcgacag
    24841 ctccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaaaac gggaaggggt
    24901 tctccttaca atctgtgaac ttcaccacaa ctccatacat cttcatcatc ctttaggcat
    24961 ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataac ggaggactta
    25021 tggctggcat cttgatacca aaatggcgca ggatatcgtg gcacgtacca tgcgcatcat
    25081 cgataccaat atcaacgtaa tggatgcccg tgggcgaatt atcggcagcg gcgatcgtga
    25141 gcgtattggt gaattgcacg aaggtgcatt gctggtactt tcacagggac gagtcgtcga
    25201 tatcgatgac gcggtggcac gtcatctgca cggtgtgcgg caagggatta atctaccgtt
    25261 acggctggaa ggtgaaattg tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg
    25321 taaatatggc gaactggtct gcatgacggc tgaaatgatg ctggaacagt cgcggttgat
    25381 gcacttgttg gctcaggata gccgtttgcg ggaggaactg gtgatgaacc tgattcaggc
    25441 agaggagaat actcccgcac ttactgaatg ggcgcaacgg ctggggatcg atctcaatca
    25501 accgcgagtg gtggctattg ttgaggtcga cagcggtcag cttggtgtgg acagcgcaat
    25561 ggcggagtta caacaactgc aaaacgcgct gactacgccc gagcgtaata atctggtggc
    25621 gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg ttgaactctt ttgggcgctg
    25681 ggatgcagaa gatcatcgta agcgagttga acaactgatt acccgcatga aagagtacgg
    25741 ccagctgcgt tttcgcgttt cactgggcaa ctattttacc ggtcctggca gtattgcccg
    25801 atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa cagcggatgc cagaaagtcg
    25861 ctgctatttt tatcaggatc tgatgttacc tgtgttactc gacagtttgc gtggcgactg
    25921 gcaggccaac gaactggcgc gaccgctggc gcggctgaaa gcgatggaca ataacggctt
    25981 gctgcgacga acgctggcgg cgtggtttcg tcacaatgtg caaccgctgg caacgtcaaa
    26041 ggcgttgttt attcatcgta ataccctgga gtatcggctt aatcgtatat cggaactgac
    26101 cgggcttgat ttgggcaatt ttgatgacag gttgctgctg tatgtggcgt tacaactgga
    26161 tgaagagcgg taggttatgc gttaaggttg aggcggcgct ggctcattcc cctcagcccg
    26221 aaggggagag gggaatgccc gaattaaccg tcaattcggg attatttacg cgttagtttt
    26281 tccagatcgg cttcaatctc gctgatctta ttggttacga ctgactccag gtgacgtaag
    26341 tcgtcgagga tcttacgctt aagatcaact tcgctgcggt cacgctggca gatttgatca
    26401 agctcatcaa tgatataccg tagattcggg ctgatttcct ggacttcttt ataaccctga
    26461 cccacaccat cagcgacgac cgttttacgc tgacgcggat atttaaactt aacgctcttg
    26521 gcgaaaaact cgcctttgtc tttctggaaa tagattttca gaatatcgtt gttggcttcc
    26581 tgccggaggc tgtaacgatc aatttcttca ggattggtaa tacccagact tttcagattg
    26641 tcgtacatag cgttacctca aaatgagtca gtaaattgtg cttattttag catttggcct
    26701 ggcccgcccg gctcgactgt gatcgcagaa agcctttttc ttccagactt ctatcatggc
    26761 gtaaaaatca aaaaattacc tgctttattg tggtgataaa attcacgttc tacaccagtt
    26821 ccagcatcgt tacttccggg cggcagttca gacgcaaacc atacaaactg cccacgccac
    26881 gggttgtgta gatgtgtcgt tcgccaaagg catttaatcc ggcgacgtaa cgtttatctt
    26941 cgacaggggc aaaaggttca ccaaccaacg gtacgcgcag ttgcccgccg tgagtatggc
    27001 cgcacagcat cagatcccag ggttcatcac gcatgacttc tttgctgtcg ggattatgcg
    27061 ccagcaccag tcttggcaga ttggcttcgc tggcgggagg cggtttgcat tgtccggccc
    27121 ataagtcacc ggtgcctacc agttcgaatt gcctgttcgg cgtggcgatc accgtagcct
    27181 ggttaaacaa caccgtgatg cccgctgatt tcaacgtctc gccaattaag tgattttttt
    27241 ctgtaccaac agggcgatcg tggttgccga agcaggcaaa cgtcggcgca cattcggcaa
    27301 ggggggagag gacatcacta aacgccgaaa aattcagcgg catatcaaat aatacgtaat
    27361 cgccgcccag taatatcaaa tcgggctttt gttctatgcc aagagcaatc gcgtcagaaa
    27421 tcaggcttaa aggaacaaaa cgagagtaat ggagatcggc cagaaaaaga attttgaatg
    27481 gtgctgcgtt gtctttaaaa aaggcgaggc ggtgacggat taattcaaac cagcctggct
    27541 cccagtaatg catataaccg aaacctgagc tcgtggctat cgtcgcggca gtagcctgca
    27601 acaatcggcg gcgtgaaatc atcgctcatc cctgcaaaaa aaatcgggca gcgtcgtgct
    27661 gcccgtgtgc atacttttag tcgatggtac gcagcagttc gttaatgccg actttgccgc
    27721 gagttttcgc gtcaactttc ttaacgataa ccgcacagta gaggctgtat ttgccatctt
    27781 ttgacggcag attacctgaa acaaccacag accccgccgg aacgcgaccg tagtggattt
    27841 cgccggtttc acggtcgtaa atacgggtgc tctgaccaat gtatacgccc atggaaatga
    27901 cggaaccttc ttcgacaatc accccttcaa ccacttcaga gcgtgcgcca atgaagcagt
    27961 tatcttcaat gatggttggg ttagcctgca gcggttccag cacgccgccg atgcccacgc
    28021 caccggaaag gtggacgttt ttaccaatct gcgcacaaga accgacggtc gcccaggtat
    28081 caaccatggt gccttcatca acatatgcgc cgatgttgac gtaagacggc atcagcacgg
    28141 tgttacgggc aataaacgca ccctgacgta ccgccgctgg tggcacaacg cggaagcctt
    28201 ctttctggaa acgtgcttcg tcgtagtcgg cgaatttcat cggcactttg tcgaagtagc
    28261 ggctttctgc cccttcgatc acctgattat cattaatacg gaaagagagc agcaccgctt
    28321 ttttcaacca ctgatgcgtc acccactgac cgtcaatttt ttccgctaca cgcagtgcac
    28381 cggaatccag cagggcgatc acctgattta ccgcttcgcg ggtaacggtg tctgcgttgg
    28441 ctggcgtgat ctcggcacgg cgttcaaaag cggtttcaat aatgttctgt aactgctgca
    28501 ttgttaaact cttttcatat cagtaaacac atcacccttt atcgtttgga ttgagggcct
    28561 ctgtcaaccg ctgatgcact tcctgctgca actcgttatt aagtgcacgc cggtcagcgg
    28621 tggcaattat gaataaatct tctactcgct cgccaatggt tgtaattcgg gcaccatgaa
    28681 gcgaaattcc cagatcggca aaaattttcc cgactctcgc cagcagtcct ggctggtcga
    28741 gcgcgataag ctcaaggaac gatttgcggt cggtatgggt cggcaaaaac gttacttcgg
    28801 tttcaacagt aaaatggcgt aatttggcgg gttggcgacg gggctgcggt ggctgccagc
    28861 tactttgcgt cagtacttgc tccagaccaa accgaataac ctcatgacga tctgcggaca
    28921 gcgggctgcc atcgggttcc agcacgataa aggtatccat cgccataccg tcgcgagtgg
    28981 tgaaaatttg tgcgtcgtga acacttaaat tgcggcggtc taattcggca cagacggcgg
    29041 caaacagata ggggcggtcc gggctccaga taaaaatctc ggtgcctcca cgcgtagcct
    29101 gcgggctaag caataccagc ggtttgctta aatcatgctg taataaatgg cgggcatgcc
    29161 aggccagttg atttgggcta tggcggacaa aatagttagc acgacagcgt gaccaaattt
    29221 ggtgcagcgc ctcttcgtcg atgttatcca tgcgcagtag tgccagtgcc tggagttggt
    29281 gatgacgaac ccgttcgcgc atatccggcg tgttttgcat tccgcgtcgt agctgctttt
    29341 cggtggcaaa atagagttcg cgcaacagac tttgcttcca gctattccac agcgtttcgt
    29401 tggtggcgca aatgtcagcc accgtcaggc ataccagata acgaagtcgg ttttctgttt
    29461 gcacttcttc ggcaaactgc ttgatgactt ccgggtcctg aatatcgcgg cgttgggcgg
    29521 tcaccgacat caacaggtgc tggcgaacca gccaggcaac cagctgtgtt tcgcgcgagt
    29581 taagcccgtg gagttcggca aaatgcacta catcctgagc accgagaatg gagtggtcgc
    29641 cgccgcgtcc tttggcgata tcgtgaaaca gcgcggcgat gaaaatcagc tcagttgacg
    29701 gcaggcgcgg ccagacgtcc acacacaacg gatggcgctg gcgcgtttct tcactggcaa
    29761 aactctccag tttcagcatc acgcggatag tatgttcatc caccgtgtag gcgtggaaca
    29821 gatcaaactg catctgcccg acgatatgcg accattgcgg catatacgcg ccgagcacgc
    29881 tatggcgatg cattggcaat agcccgcgcc gcaccgctcc ggggtgacgc agaatgctca
    29941 aaaacagttt tcgtgcttcc ggaatattac acagcggttg ttgcagatgg cgacgggcat
    30001 ggcgtaactg gcgcagcgtg gtggagtaaa tgccggtgat cgcactgttg cgcaccatgg
    30061 tgtagaacat acgcaagatg gcttccggct ggcgcataaa tagtgtttca tcacgcaggt
    30121 cgattagcgt accgcgtagc tgaaactcat cgtcgagtgg acgtggtttt tcgtcggcgg
    30181 gaagggcgag gatggcttca tcgaacagtt gcagcagcat ctggttgagt tcactgacgc
    30241 ggcgtgtaac gcggaagtaa tccttcatca tccgctcgac cggttcgttg ccttcaccac
    30301 tgtaattcag acgctgagcg acgctaagtt ggcgatcgaa taacagacga ttatcgtaac
    30361 ggctgacgac cagatgcagg gcaaagcgaa tacgccacag tatatgcaga cattcgttta
    30421 attccgcccg ctccgctgag gttaagaagc caaacccaac catttcatcc agcgatgttg
    30481 cgccaaaatg acggcgggcc acccattgca gagtgtggat atcgcgcaag ccgccagggc
    30541 tacttttgat gtctggttca agattgtagc tggtgccgtg gtaacgctga tggcgctggt
    30601 tctgttcttc aactttcgcc gcgtagaact tgtcggaagg ccagaatcct tcgctaaaaa
    30661 tatgtttttg cagttcgagg aagagcgcaa catcgccaat taataagcgg gattcgatta
    30721 aattggtggc gacggttaaa tccgataacc cttccagcat gcactcttca agcgtgcgca
    30781 cactatgacc gacttccagc tttacatccc agagcagcgt taacagctcg cccacttttt
    30841 gcgcctgatc gtccgggagc tttttacggc ttaaaatcag taaatcgatg tcagaaagtg
    30901 gatgcagctc gccacgaccg tagccaccga cggcgaccaa tgccaggtcg gcaatctggc
    30961 tgaatcccgc ttcaatccat aatcgttgca ggagctggtc gataaactcg gtgcgcgcct
    31021 caatcaactg ttctgcagag atcccattgt caaaggcatc acccagccaa cgctggaaag
    31081 tatcgatatg ggcttttatc ccaccgacgg ttaattcatc acggggccag acgcatggat
    31141 tttgcggttg accgggcagg gtggggagag cggtgtttgc gtactgttct ggaagggtat
    31201 tcattgtgcg ccacccataa gattaaatta tcgcattaaa aaagccggca ttcgccggct
    31261 tcatcttatt cgtcgtgcga gattatcgcc gggatggtgt catccttgcg tagcgtcaga
    31321 atttcgcagc cgttatcagt caccacaata gtatgctcat attgtgcaga caagctgcga
    31381 tctttggttt ttaccgtcca gccatctttc atggtgcgga tctctttttt acccgcgttg
    31441 accattggct cgatggtgaa cgtcatccca ggtttcagta cgacgttggt ttcacgggag
    31501 tcatagtgca gcacctgcgg ttcttcatgg aagccgcgac caataccgtg tccgcaatat
    31561 tcacgaacga cggagaagcc ttctgcttcg acaaatttct gaatcgccgc accgatttcg
    31621 cgcagattaa tgcctggttt gaccatgcgc agcgccaggt acaggctttc ttgcgtgatg
    31681 cggcacagac gttcgcccat gatggtcggc ttaccgacga taaacatttt cgaggtatcg
    31741 ccgtggaaac catctttgat tacggtgaca tcaatgttaa cgatatcgcc atctttcagc
    31801 agcttcgcat cgtccgggat accgtggcac accacttcat taatagagat gcaaacggat
    31861 ttcggatagc cgtgatagcc gaggcaggca gaaaccgtgt gttgttcatt aacaatgtaa
    31921 tcattacaga tgcgatccag ctcgccggtg ctgacgcccg gtttaacata cggttcgatc
    31981 atctccagca cttcggcagc cagtcggcca gcgacgcgca ttttttcgat atcttctggg
    32041 gtcttgattg agatagccat taattctgtc catcagcgtc ggtgataccg acaatatata
    32101 tgtaagtgcc gtcaatggta tcacacccgg gcaaattgag aatcattctg aatttcgcca
    32161 aacgtgccac tgaacgtttt ctataataga aaattcaacg tctgatgctg tacacagcgc
    32221 caacaattat tggtgtccat gacgtatttg tggtataaag cgcgccggac ttccgatcca
    32281 tttcgtatac acagactgga cggaagcgac aatctcactt tgtgtaacaa cacacacgta
    32341 tcggcacata ttccggggtg ccctttgggg tcggtaatat gggatacgtg gaggcataac
    32401 cccaactttt atatagaggt tttaatcatg gcaactgttt ccatgcgcga catgctcaag
    32461 gctggtgttc acttcggtca ccagacccgt tactggaacc cgaaaatgaa gccgttcatc
    32521 ttcggtgcgc gtaacaaagt tcacatcatc aaccttgaga aaactgtacc gatgttcaac
    32581 gaagctctgg ctgaactgaa caagattgct tctcgcaaag gtaaaatcct tttcgttggt
    32641 actaaacgcg ctgcaagcga agcggtgaaa gacgctgctc tgagctgcga ccagttcttc
    32701 gtgaaccatc gctggctggg cggtatgctg actaactgga aaaccgttcg tcagtccatc
    32761 aaacgtctga aagacctgga aactcagtct caggacggta ctttcgaaaa gctgaccaag
    32821 aaagaagcgc tgatgcgcac tcgtgagctg gagaaactgg aaaacagcct gggcggtatc
    32881 aaagacatgg gcggtctgcc ggacgctctg tttgtaatcg atgctgacca cgaacacatt
    32941 gctatcaaag aagcaaacaa cctgggtatt ccggtatttg ctatcgttga taccaactct
    33001 gatccggacg gtgttgactt cgttatcccg ggtaacgacg acgcaatccg tgctgtgacc
    33061 ctgtacctgg gcgctgttgc tgcaaccgta cgtgaaggcc gttctcagga tctggcttcc
    33121 caggcggaag aaagcttcgt agaagctgag taataaggct tgataactcc cccaaaatag
    33181 ttcgagttgc agaaaggcgg caagctcgag aattcccggg agcttacatc agtaagtgac
    33241 cgggatgagc gagcgaagac aacgcatctg cggcgcgaaa tatgaagggg gagagccctt
    33301 atagaccagg tagtacacgt ttggttaggg ggcctgcata tggccccctt tttcactttt
    33361 atatctgtgc ggtttaatgc cgggcagatc acatctccga ggattttaga atggctgaaa
    33421 ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg atggattgca
    33481 aaaaagcact gactgaagct aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt
    33541 ccggtgctat taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa
    33601 ccaaaatcga cggcaactac ggcatcattc tggaagttaa ctgccagact gacttcgttg
    33661 caaaagacgc tggtttccag gcgttcgcag acaaagttct ggacgcagct gttgctggca
    33721 aaatcactga cgttgaagtt ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag
    33781 cgaaaattgg tgagaacatc aacattcgcc gcgttgctgc gctggaaggc gacgttctgg
    33841 gttcttatca gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag
    33901 agctggttaa acacatcgct atgcacgttg ctgcaagcaa gccagaattc atcaaaccag
    33961 aagacgtatc cgctgaagtg gtagaaaaag aataccaggt acagctggat atcgcgatgc
    34021 agtctggtaa gccgaaagaa atcgcagaga aaatggttga aggccgcatg aagaaattca
    34081 ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga accaagcaaa actgttggtc
    34141 agctgctgaa agagcataac gctgaagtga ctggcttcat ccgcttcgaa gtgggtgaag
    34201 gcatcgagaa agttgagact gactttgcag cagaagttgc tgcgatgtcc aagcagtctt
    34261 aattatcaaa aaggagccgc ctgagggcgg cttctttttg tgcccatctt gtaaattcag
    34321 ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg tcgctagtat taattcattt
    34381 caatcgttga cagtctcagg aaagaaacat ggctaccaat gcaaaacccg tctataaacg
    34441 cattctgctt aagttgagtg gcgaagctct gcagggcact gaaggcttcg gtattgatgc
    34501 aagcatactg gatcgtatgg ctcaggaaat caaagaactg gttgaactgg gtattcaggt
    34561 tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat
    34621 gaaccgcgtt gtgggcgacc acatggggat gctggcgacc gtaatgaacg gcctggcaat
    34681 gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa
    34741 tggcgtgtgc gacagctaca gctgggcaga agctatcagc ctgttgcgca acaaccgtgt
    34801 ggtgatcctc tctgccggta caggtaaccc gttctttacc accgactcag cagcttgcct
    34861 gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca accaaagttg acggcgtgtt
    34921 taccgctgat ccggcgaaag atccaaccgc aaccatgtac gagcaactga cttacagcga
    34981 agtgctggaa aaagagctga aagtcatgga cctagcggcc ttcacgctgg ctcgtgacca
    35041 taaattaccg attcgtgttt tcaatatgaa caaaccgggt gcgctgcgcc gtgtggtaat
    35101 gggtgaaaaa gaagggactt taatcacgga ataattcccg tgatggataa ataagggtaa
    35161 gattccgcgt aagtatcgcg ggggcgttag tctggttata aggcgttatt gttgcaggca
    35221 gtttggtcac ggccagcgcg cagcaaccgg agcgtacaaa agtacgtgag gatggcgagc
    35281 actgctcggg gccaaaatgg caaataaaat agcctaataa tccagacgat tacccgtaat
    35341 atgtttaatc agggctatac ttagcacact tccactgtgt gtgactgtct ggtctgactg
    35401 agacaagttt tcaaggattc gtaacgtgat tagcgatatc agaaaagatg ctgaagtacg
    35461 catggacaaa tgcgtagaag cgttcaaaac ccaaatcagc aaaatacgca cgggtcgtgc
    35521 ttctcccagc ctgctggatg gcattgtcgt ggaatattac ggcacgccga cgccgctgcg
    35581 tcagctggca agcgtaacgg tagaagattc ccgtacactg aaaatcaacg tgtttgatcg
    35641 ttcaatgtct ccggccgttg aaaaagcgat tatggcgtcc gatcttggcc tgaacccgaa
    35701 ctctgcgggt agcgacatcc gtgttccgct gccgccgctg acggaagaac gtcgtaaaga
    35761 tctgaccaaa atcgttcgtg gtgaagcaga acaagcgcgt gttgcagtac gtaacgtgcg
    35821 tcgtgacgcg aacgacaaag tgaaagcact gttgaaagat aaagagatca gcgaagacga
    35881 cgatcgccgt tctcaggacg atgtacagaa actgactgat gctgcaatca agaaaattga
    35941 agcggcgctg gcagacaaag aagcagaact gatgcagttc tgatttcttg aacgacaaaa
    36001 acgccgctca gtagatcctt gcggatcggc tggcggcgtt ttgcttttta ttctgtctca
    36061 actctggatg tttcatgaag caactcacca ttctgggctc gaccggctcg attggttgca
    36121 gcacgctgga cgtggtgcgc cataatcccg aacacttccg cgtagttgcg ctggtggcag
    36181 gcaaaaatgt cactcgcatg gtagaacagt gcctggaatt ctctccccgc tatgccgtaa
    36241 tggacgatga agcgagtgcg aaacttctta aaacgatgct acagcaacag ggtagccgca
    36301 ccgaagtctt aagtgggcaa caagccgctt gcgatatggc agcgcttgag gatgttgatc
    36361 aggtgatggc agccattgtt ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg
    36421 cgggtaaaac cattttgctg gccaataaag aatcactggt tacctgcgga cgtctgttta
    36481 tggacgccgt aaagcagagc aaagcgcaat tgttaccggt cgatagcgaa cataacgcca
    36541 tttttcagag tttaccgcaa cctatccagc ataatctggg atacgctgac cttgagcaaa
    36601 atggcgtggt gtccatttta cttaccgggt ctggtggccc tttccgtgag acgccattgc
    36661 gcgatttggc aacaatgacg ccggatcaag cctgccgtca tccgaactgg tcgatggggc
    36721 gtaaaatttc tgtcgattcg gctaccatga tgaacaaagg tctggaatac attgaagcgc
    36781 gttggctgtt taacgccagc gccagccaga tggaagtgct gattcacccg cagtcagtga
    36841 ttcactcaat ggtgcgctat caggacggca gtgttctggc gcagctgggg gaaccggata
    36901 tgcgtacgcc aattgcccac accatggcat ggccgaatcg cgtgaactct ggcgtgaagc
    36961 cgctcgattt ttgcaaacta agtgcgttga catttgccgc accggattat gatcgttatc
    37021 catgcctgaa actggcgatg gaggcgttcg aacaaggtca ggcagcgacg acagcattga
    37081 atgccgcaaa cgaaatcacc gttgctgctt ttcttgcgca acaaatccgc tttacggata
    37141 tcgctgcgtt gaatttatcc gtactggaaa aaatggatat gcgcgaacca caatgtgtgg
    37201 acgatgtgtt atctgttgat gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc
    37261 tcgcaagctg aggataatcc ggctacagag agtcgcgcta tttgttagcg tagggcttca
    37321 gtgatatagt ctgcgccatc tgatcgtaag tagttggctt tataaggtca ggtatgccgt
    37381 ggttttacac ggcttttttt tgtataggct tcagtattcc tgagtaccgt aaaccctgtc
    37441 agggaataaa aaacgcgtga tgttgtctgc tactcaacca cttagcgaaa aattgccagc
    37501 gcatggctgc cgtcatgttg cgatcattat ggacggcaat ggtcgctggg caaaaaagca
    37561 agggaagatt cgtgcctttg ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc
    37621 ttttgcggcc aacaacggta ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg
    37681 gaaccgacca gcgcaggaag tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag
    37741 cgaagtaaaa agtctgcacc gacataacgt gcgtctgcgt attattggcg ataccagtcg
    37801 ctttaactcg cgtttgcaag aacgtattcg taaatctgaa gcgctaacag ccgggaatag
    37861 cggtctgacg ctgaatattg cggcgaacta cggtggacgt tgggatatag tccagggagt
    37921 caggcaactg gctgaaaagg tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga
    37981 gatgctaaac cagcatgtct gtatgcatga actggcccct gtagatttag taattaggac
    38041 tgggggggag catcgcatta gtaacttttt gctttggcaa attgcctatg ccgaacttta
    38101 ctttacagat gttctctggc ccgatttcga tgaacaagac tttgaagggg cgttaaatgc
    38161 ctttgctaat cgagagcgtc gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg
    38221 ggggtcgctt ttgctgaagt atcgcctgat atctgctttt gtgttaatac ccgtcgtcat
    38281 cgcggcgttg tttctgttgc cgccggtggg gttcgccatc gtaacgctgg tggtctgcat
    38341 gctggcagcg tgggaatggg ggcagcttag cggttttacc actcgttcgc agcgagtatg
    38401 gttggcggtg ttatgcgggt tattgttggc gctgatgctt tttctgttgc cggaatatca
    38461 ccgaaatatt catcaaccgc tggttgaaat ctcactttgg gcttcgttgg gttggtggat
    38521 tgtcgcgcta ttgctggtgc tgttttaccc aggttccgca gcaatctggc gtaactctaa
    38581 aacattgcgc cttatttttg gcgtgctaac cattgttccc ttcttctggg gcatgctggc
    38641 gttacgggcc tggcactacg acgagaatca ttacagtggc gcaatatggc tgctctatgt
    38701 catgatcctg gtatggggcg ctgactctgg cgcatatatg tttggcaaat tgtttggtaa
    38761 acataagctg gcaccgaagg tttctccggg taaaacctgg caaggcttta taggtggact
    38821 agcgaccgca gcggtaatct catggggtta tggcatgtgg gcgaatctcg acgtcgctcc
    38881 cgtcacctta ctcatttgct ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac
    38941 cgagagtatg tttaagcgtg aagcaggaat taaggacagc ggtcatttaa ttccaggaca
    39001 cggtggtatt ttagatcgta ttgatagcct gacggctgcg gtaccggtct ttgcttgctt
    39061 gttgttactg gtattcagga cgctttaacg gaaggtaata tgctgagttt tctctgggat
    39121 ttggcttcgt tcatcgttgc actgggtgta cttatcaccg tgcatgaatt tggtcatttc
    39181 tgggttgccc ggcgttgtgg tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg
    39241 ctctggcggc gaactgataa gctcggcacc gaatatgtta tcgccctgat cccgctgggc
    39301 ggttatgtca aaatgctgga tgagcgcgca gaaccggtcg ttccggaact ccgccaccat
    39361 gccttcaata ataaatctgt cggccaacga gcggcgatta ttgccgcagg tccggttgca
    39421 aacttcattt ttgctatctt tgcctactgg ctggttttta ttattggtgt gcctggcgta
    39481 cgtccggtgg ttggtgaaat agcagccaat tcgatagctg cggaagcaca aattgcacca
    39541 ggtacggaac taaaagccgt agatggtatc gaaacgcctg attgggatgc cgtgcgtttg
    39601 cagttggtcg ataaaattgg cgatgaaagc accaccatta cagtagcgcc atttggcagc
    39661 gaccaacggc gggatgtaaa gctcgattta cgtcactggg cgtttgagcc tgataaagaa
    39721 gatccggtat cttcgctggg gattcgtcct cgtgggccgc aaattgaacc tgtactggaa
    39781 aatgtgcaac caaactcggc ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt
    39841 aaagtcgatg gtcagccctt aacgcagtgg gtgacctttg tgatgcttgt ccgggataac
    39901 ccgggtaaat ccttagcgtt agaaatcgaa aggcagggaa gtcccttgtc tttgacatta
    39961 atcccggaga gtaaaccggg taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa
    40021 gtcattcctt tgccagatga gtataaagtt gtacgccagt atgggccgtt caacgccatc
    40081 gtcgaagcca cggacaaaac gtggcagctg atgaagctga cggtcagtat gctgggaaaa
    40141 ttgatcaccg gtgatgtgaa actgaacaac ctcagtgggc cgatctctat cgccaagggg
    40201 gctgggatga cagcggaact cggggtagtt tattacctgc cgtttcttgc gcttattagc
    40261 gtgaacttag ggataattaa cctgtttccg ttgcccgtac ttgacggggg gcatctgctg
    40321 ttccttgcga tcgaaaagat caagggcgga ccggtatccg agcgggttca agacttttgt
    40381 tatcgcattg gctcgattct gctggtgctg ttaatggggc ttgcactttt caatgatttc
    40441 tctcggttat gagagttagt taggaagaac gcataataac gatggcgatg aaaaagttgc
    40501 tcatagcgtc gctgctgttt agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga
    40561 aagatattca tttcgaaggc cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc
    40621 cggtgcgcac aggcgacacg gttaatgatg aagatatcag taataccatt cgcgctctgt
    40681 ttgctaccgg caactttgag gatgttcgcg tccttcgtga tggtgatacc cttctggttc
    40741 aggtaaaaga acgtccgacc attgccagca ttactttctc cggtaacaaa tcggtgaaag
    40801 atgacatgct gaagcaaaac ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc
    40861 gcaccaccat tgccgatatc gagaaaggtc tggaagactt ctactacagc gtcggtaaat
    40921 atagcgccag cgtaaaagct gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac
    40981 tggtgttcca ggaaggtgtg tcagctgaaa tccagcaaat taacattgtt ggtaaccatg
    41041 ctttcaccac cgacgaactg atctctcatt tccaactgcg tgacgaagtg ccgtggtgga
    41101 acgtggtagg cgatcgtaaa taccagaaac agaaactggc gggcgacctt gaaaccctgc
    41161 gcagctacta tctggatcgc ggttatgccc gtttcaacat cgactctacc caggtcagtc
    41221 tgacgccaga taaaaaaggt atttacgtca cggtgaacat taccgaaggc gatcagtaca
    41281 agctttctgg cgttgaagtg agcggcaacc ttgccgggca ctccgctgaa attgagcagc
    41341 tgactaagat cgagccgggc gagctgtata acggcaccaa agtgaccaag atggaagatg
    41401 acatcaaaaa gcttctcggt cgctatggtt atgcctatcc gcgcgtacag tcgatgcctg
    41461 aaattaacga tgccgacaaa accgttaaat tacgcgtgaa cgttgatgcg ggtaaccgtt
    41521 tctacgtgcg taagatccgt tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc
    41581 gcgaaatgcg tcagatggaa ggtgcatggc tgggaagcga tctggtcgat cagggtaagg
    41641 agcgtctgaa tcgtctgggc ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg
    41701 gtagcccgga ccaggtagat gtcgtctaca aggtaaaaga gcgcaacacc ggtagcttca
    41761 acttcgggat tggttacggt actgaaagtg gcgtgagctt ccaggctggt gtgcagcagg
    41821 ataactggtt aggtacaggt tatgctgttg gtatcaacgg gaccaaaaac gattaccaga
    41881 cctatgctga actgtcggta accaacccgt acttcactgt agatggtgta agcctcggtg
    41941 gtcgtctctt ctataatgac ttccaggcag atgacgccga cctgtccgac tataccaaca
    42001 agagttatgg tacagacgtg acgttgggct tcccgatcaa cgaatataac tcgctgcgtg
    42061 caggtctggg ttatgtacat aactccctgt ccaacatgca gcctcaggtt gcgatgtggc
    42121 gttatctgta ctctatgggt gaacatccga gcacttctga tcaggataat agcttcaaaa
    42181 cggacgactt cacgttcaac tatggttgga cctataacaa gcttgaccgt ggttacttcc
    42241 cgacagatgg ttcacgtgtc aacctgaccg gtaaagtgac cattcctgga tcggataacg
    42301 aatactacaa agtgacgtta gacacggcga cttatgtgcc gatcgatgac gatcacaaat
    42361 gggttgttct ggggcgtacc cgctggggtt atggtgatgg tttaggcggc aaagagatgc
    42421 cgttctacga gaacttctat gccggtggtt ccagcaccgt gcgtggcttc cagtccaata
    42481 caattggtcc gaaagcagtt tacttcccgc atcaggccag taattatgat ccggactatg
    42541 attacgaatg tgcgactcag gacggcgcga aagacctgtg taaatcggat gatgctgtag
    42601 gcggtaacgc catggcggtt gccagcctcg agttcatcac cccgacgccg tttattagcg
    42661 ataagtatgc taactcggtt cgtacttcct tcttctggga tatgggtacc gtttgggata
    42721 caaactggga ttccagccaa tattctggat atccggacta tagtgatcca agcaatatcc
    42781 gtatgtctgc gggtatcgca ttacaatgga tgtccccatt ggggccgttg gtgttctcct
    42841 acgcccagcc gttcaaaaag tacgatggag acaaggcaga acagttccag tttaacatcg
    42901 gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg tagtgtagcg atgactttag
    42961 gcgatcaata taagatcgcc gggccacgca aagaactgca ccctccggtg caaatgggat
    43021 ggtaaggagt ttattgtgaa aaagtggtta ttagcggcag gtctcggttt agcactggca
    43081 acttctgctc aggcggctga caaaattgca atcgtcaaca tgggcagcct gttccagcag
    43141 gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc
    43201 gaactgcagc gtatggaaac cgatctgcag gctaaaatga aaaagctgca gtccatgaaa
    43261 gcgggcagcg atcgcactaa gctggaaaaa gacgtgatgg ctcagcgcca gacttttgct
    43321 cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa
    43381 ctggttactc gtatccagac tgctgtgaaa tccgttgcca acagccagga tatcgatctg
    43441 gttgttgatg caaacgccgt tgcttacaac agcagcgatg taaaagacat cactgccgac
    43501 gtactgaaac aggttaaata agtaatgcct tcaattcgac tggctgattt agcgcagcag
    43561 ttggatgcag aactacacgg tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa
    43621 tctgcacaaa caggtcacat tacgttcatg gttaacccaa aataccgtga gcatttaggc
    43681 ttgtgccagg cgtccgcggt tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc
    43741 gcgctggtag tgaagaatcc ctacctgact tacgcgcgca tggcgcaaat tttagatacc
    43801 acgccgcagc ccgcgcagaa cattgcaccc agtgcggtga tcgacgcgac ggcgaagctg
    43861 ggtaacaacg tatcgattgg cgctaacgcg gtgattgagt ccggcgttga actgggcgat
    43921 aacgtgatta tcggtgccgg ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg
    43981 cgtctctggg cgaacgtaac catttaccat gagatccaga tcggtcagaa ttgcctgatc
    44041 cagtccggaa cagtggtagg cgcagacggc tttggttatg ccaacgatcg tggtaactgg
    44101 gtgaagatcc cacagattgg tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc
    44161 acaaccatcg atcgcggcgc gctggatgac actattattg gcaatggcgt gatcattgat
    44221 aaccagtgcc agattgcaca taacgtcgtg attggcgaca atacggcggt tgccggtggc
    44281 gtcattatgg cgggcagcct gaaaattggt cgttactgca tgatcggcgg agccagcgta
    44341 atcaacgggc atatggaaat atgcgacaaa gtgacggtta cgggcatggg tatggtgatg
    44401 cgtcccatca ctgaaccagg cgtctattcc tcaggcattc cgctgcaacc caacaaagtc
    44461 tggcgcaaaa ccgctgcact ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg
    44521 cttgagcgca aggttaatca acaagactaa cgttccatct tttgttcgcc aaactttacg
    44581 gcctgtctca ttcttacgat tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg
    44641 acaggaagag tatcttgact actaacactc atactctgca gattgaagag attttagaac
    44701 ttctgccgca ccgtttcccg ttcttactgg tggatcgcgt gctggatttt gaagaaggtc
    44761 gttttctgcg cgcagtaaaa aatgtctctg tcaatgagcc attcttccag ggccatttcc
    44821 ctggaaaacc gattttcccg ggtgtgctga ttctggaagc aatggcacag gcaacaggta
    44881 ttctggcgtt taaaagcgta ggaaaactgg aaccgggtga gctgtactac ttcgctggta
    44941 ttgacgaagc gcgcttcaag cgcccggtcg tgcctggcga tcaaatgatc atggaagtca
    45001 ctttcgaaaa aacgcgccgc ggcctgaccc gttttaaagg ggttgctctg gtcgatggta
    45061 aagtagtttg cgaagcaacg atgatgtgtg ctcgtagccg ggaggcctga tacgtgattg
    45121 ataaatccgc ctttgtgcat ccaaccgcca ttgtggaaga gggcgcgtca attggcgcga
    45181 acgcacacat tggtcctttt tgtatcgttg gaccccatgt cgaaattggt gagggtaccg
    45241 tactgaaatc tcacgttgtc gtgaatggtc atactaaaat tggccgcgat aatgagattt
    45301 atcagttcgc ctccatcggc gaagttaacc aggatctgaa atatgctggc gaaccgaccc
    45361 gtgtggaaat cggcgatcgt aaccgcattc gcgaaagcgt caccattcat cgtggcacag
    45421 tccagggcgg tggattgacg aaggtgggca gcgacaactt actgatgatc aacgcgcaca
    45481 ttgcgcacga ttgtacggta ggtaaccgct gtattctcgc caacaacgca acgctggcgg
    45541 gtcacgtatc ggttgacgac ttcgcgatca tcggcggcat gaccgcagtc catcagttct
    45601 gcatcattgg tgcgcacgtg atggttggcg gctgctccgg tgtggcgcag gacgtccctc
    45661 cttatgtcat tgcgcagggt aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga
    45721 agcgccgcgg attcagccgt gaggcgatta ccgctatccg caatgcgtat aagctgattt
    45781 atcgtagcgg taaaacgctc gatgaagtga aaccggaaat tgctgaactg gcggaaacat
    45841 atccggaagt gaaagccttt accgatttct ttgcacgctc aacgcgcggt ctgattcgtt
    45901 aatgactgaa cagcgtccat taacgattgc cctggtcgcc ggagaaacct ccggcgatat
    45961 cctgggggcc ggtttaatcc gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg
    46021 tgttgccggg ccacgaatgc aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact
    46081 ggcggtgatg ggcattgttg aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc
    46141 cgatctgaca aagcgttttg gcgaactgaa gccagatatt tttgttggta ttgatgcgcc
    46201 tgacttcaat attactcttg aaggtaacct caaaaagcag ggtatcaaaa ccattcatta
    46261 cgtcagtcca tccgtctggg cgtggcgaca gaaacgcgtt ttcaaaatag gcagagccac
    46321 cgatctggtg ctcgcatttc tgcctttcga aaaagcgttt tatgacaaat acaatgtacc
    46381 gtgccgcttt atcggtcata ccatggctga tgccatgccg ttagatccag ataaaaatgc
    46441 cgcccgtgat gtgctgggga tccctcacga tgcccactgc ctggcgttgc taccagggag
    46501 ccgtggtgca gaagttgaaa tgcttagtgc cgatttcctg aaaacggccc agcttttgcg
    46561 ccagacatat ccggatctcg aaatcgtggt gccactggtg aatgccaaac gccgcgagca
    46621 gtttgaacgc atcaaagctg aagtcgcgcc agacctttca gttcatttgc tggatgggat
    46681 gggccgtgag gcgatggtcg ccagcgatgc ggcgctactg gcgtcgggta cggcagccct
    46741 ggagtgtatg ctggcgaaat gcccgatggt ggtgggatat cgcatgaagc cttttacctt
    46801 ctggttggcg aagcggctgg tgaaaactga ttatgtctcg ctgccaaatc tgctggcggg
    46861 tagagagtta gtcaaagaat tattgcagga agagtgtgag ccgcaaaaac tggctgcggc
    46921 gctgttaccg ctgttggcga acgggaaaac cagccacgcg atgcacgata ccttccgtga
    46981 actgcatcag cagatccgct gcaatgccga tgagcaggcg gcacaagccg ttctggagtt
    47041 agcacaatga tcgaatttgt ttatccgcac acgcagctgg ttgcgggtgt ggatgaagtc
    47101 ggacgcgggc cgttagttgg cgcggtcgtc accgctgcgg tgatccttga cccggcgcgc
    47161 ccgattgccg ggctgaatga ttccaaaaag ctgagcgaaa aacgccgtct ggcgctctat
    47221 gaagagatca aagagaaagc gttgagctgg agtctgggcc gcgcggaacc ccacgaaatc
    47281 gacgagctga acattcttca tgcgaccatg ctggcgatgc agcgtgccgt cgctgggctg
    47341 catattgcgc cggaatatgt gttgattgat ggtaaccgct gcccgaaatt accgatgcct
    47401 gcgatggctg tggtgaaagg cgatagccgc gtaccggaaa tcagtgccgc gtctatcctg
    47461 gcgaaagtga cgcgtgacgc cgaaatggcg gcgctggata ttgttttccc gcaatatggt
    47521 tttgcccaac acaaagggta cccaaccgct tttcatctgg aaaaactggc tgaacacggc
    47581 gcgaccgaac accatcggcg cagctttggg cctgtcaaac gcgcactggg acttgcgtcc
    47641 tgattcttgt gtcgagatta agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta
    47701 cacctgcggg tgcacagcga ctactcgatg atcgatggcc tggccaaaac cgcaccgttg
    47761 gtaaaaaagg cggcggcgtt gggtatgcca gcactggcga tcaccgattt caccaacctt
    47821 tgtggtctgg tgaagttcta cggagcggga catggcgcag ggattaagcc tatcgtcggg
    47881 gcagatttta acgtccagtg cgacctgctg ggtgatgagt taacccacct gacggtactg
    47941 gcggcgaaca ataccggcta tcagaatctg acgttgctga tctcaaaagc gtatcagcgc
    48001 gggtacggtg ccgccgggcc gatcatcgat cgcgactggc ttatcgaatt aaacgaaggg
    48061 ttgatccttc tttccggcgg acgcatgggc gacgtcggac gcagtctttt gcgtggtaac
    48121 agagcgctgg tagatgagtg tgtcgcgttt tatgaagaac acttcccgga tcgctatttt
    48181 ctcgagctga tccgcaccgg caggccggat gaagaaagct atctgcacgc ggcggtggaa
    48241 ctggcggaag cgcgcggttt gcccgtcgtg gcgaccaacg acgtgcgctt tatcgacagc
    48301 agcgactttg acgcacacga aatccgcgtc gcgatccacg acggctttac cctcgacgat
    48361 cctaaacgcc cgcgtaacta ttcgccgcag caatatatgc gtagcgaaga ggagatgtgt
    48421 gagctgtttg ccgacatccc cgaagccctt gccaacaccg ttgagatcgc caaacgctgt
    48481 aacgtaaccg tgcgtcttgg tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc
    48541 accgaagatt atctggtcaa gcgtgcaaaa gagggcctgg aagagcgtct ggccttttta
    48601 ttccctgacg aggaagaacg tgttaagcgc cgcccggaat atgacgaacg tctggagact
    48661 gaacttcagg ttatcaacca gatgggcttc ccgggctact tcctcatcgt tatggaattt
    48721 atccagtggt cgaaagataa cggcgtaccg gtagggccag gccgtggctc cggtgcgggt
    48781 tcactggtgg cctacgcgct gaaaatcacc gacctcgatc cgctggaatt tgacctgctg
    48841 ttcgaacgtt tccttaaccc ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt
    48901 atggagaaac gcgatcaggt tatcgagcac gtagcggaca tgtacggtcg tgatgcggta
    48961 tcgcagatca tcaccttcgg tacaatggcg gcgaaagcgg tgatccgcga cgtaggccgc
    49021 gtgctggggc atccgtacgg ctttgtcgat cgtatctcga aactgatccc gcccgatccg
    49081 gggatgacgc tggcgaaagc gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg
    49141 gatgaagaag ttaaggcgct gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac
    49201 gccggtaagc acgccggtgg ggtggttatc gcgccgacca aaattaccga ttttgcgccg
    49261 ctttactgcg atgaagaggg caaacatccg gtcacccagt ttgataaaag cgacgttgaa
    49321 tacgccggac tggtgaagtt cgacttcctt ggtttgcgta cgctcaccat catcaactgg
    49381 gcgctggaga tgatcaacaa gcggcgggcg aagaatggcg agccgccgct ggatatcgct
    49441 gcgatcccgc tggatgataa gaaaagcttc gacatgctgc aacgctcgga aaccacggcg
    49501 gtattccagc ttgaatcgcg cggcatgaag gacctgatca agcgtctaca acctgactgc
    49561 ttcgaagata tgatcgccct agtggcactg ttccgccccg gtccgttgca atcagggatg
    49621 gtggataact ttatcgaccg taaacatggt cgtgaagaga tctcctatcc ggacgtacag
    49681 tggcagcatg aaagcctgaa accggtactg gagccaacct acggcattat cctgtatcag
    49741 gaacaggtca tgcagattgc gcaggtgctt tctggttata ccctcggtgg cgcggatatg
    49801 ctgcgtcgtg cgatgggtaa gaaaaagccg gaagagatgg ctaagcaacg ttctgtattt
    49861 gctgaaggtg cagaaaagaa cggaatcaac gctgaactgg cgatgaaaat cttcgacctg
    49921 gtggagaaat tcgctggtta cggatttaac aaatcgcact ctgcggccta tgctttggtg
    49981 tcatatcaaa cgttatggct gaaagcacac tatcctgcgg agtttatggc ggcggtaatg
    50041 accgccgata tggacaacac cgagaaggtg gtgggtctgg tggatgagtg ctggcggatg
    50101 gggctgaaaa tcctgccacc agatataaac tccggtcttt accatttcca cgtcaacgac
    50161 gacggcgaaa tcgtgtatgg tattggcgcg atcaaagggg tcggtgaagg tccgattgag
    50221 gccatcatcg aagcccgtaa taaaggcggc tacttccgcg aactgtttga tctctgcgcc
    50281 cgtaccgaca ccaaaaagtt gaaccgtcgc gtgctggaaa aactgatcat gtccggggcg
    50341 tttgaccgtc ttgggccaca ccgcgcggcg ctgatgaact cgctgggtga tgcgttaaaa
    50401 gcggcagatc aacacgcgaa agcggaagct atcggtcagg ccgatatgtt cggcgtgctg
    50461 gccgaagagc cggaacaaat tgaacaatcc tacgccagct gccaaccgtg gccggagcag
    50521 gtggtattag atggggaacg tgaaacgtta ggcctgtacc tgaccggaca ccctatcaac
    50581 cagtatttaa aagagattga gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg
    50641 acagaacgtg gtaaagtcat cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc
    50701 accaagcgcg gcaatcgtat cggtatctgc acgctggatg accgttccgg gcggctggaa
    50761 gtgatgttgt ttactgacgc cctggataaa taccagcaat tgctggaaaa agaccgcata
    50821 cttatcgtca gcggacaggt cagctttgat gacttcagcg gtgggcttaa aatgaccgct
    50881 cgcgaagtga tggatattga cgaagcccgg gaaaaatatg ctcgcgggct tgctatctcg
    50941 ctgacggaca ggcaaattga tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc
    51001 caccgctctg ggacaattcc agtacatctc tactatcaga gggcggatgc acgcgcgcgg
    51061 ttgcgttttg gcgcgacgtg gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt
    51121 ggcctcattg gttcggagca ggtggaactg gagtttgact aatacaggaa tactatgagt
    51181 ctgaatttcc ttgattttga acagccgatt gcagagctgg aagcgaaaat cgattctctg
    51241 actgcggtta gccgtcagga tgagaaactg gatattaaca tcgatgaaga agtgcatcgt
    51301 ctgcgtgaaa aaagcgtaga actgacgcgt aaaatcttcg ccgatcttgg tgcatggcag
    51361 attgcgcaac tggcacgcca tccacagcgt ccttataccc tggattacgt tcgcctggca
    51421 tttgatgaat ttgacgaact ggctggcgac cgcgcgtatg cagacgataa agctatcgtc
    51481 ggtggtatcg cccgtcttga cggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt
    51541 gaaaccaaag aaaaaattcg ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa
    51601 gcactgcgtc tgatgcaaat ggctgaacgc tttaagatgc ctatcatcac ctttatcgac
    51661 accccggggg cttatccggg cgtgggcgca gaagaacgtg gtcagtctga agccattgca
    51721 cgcaacctgc gtgaaatgtc tcgcctcggt gtaccggtag tttgtacggt tatcggtgaa
    51781 ggtggttctg gcggcgcgct ggcgattggc gtgggcgata aagtgaatat gctgcaatac
    51841 agcacctatt ccgttatctc gccggaaggt tgtgcgtcca ttctgtggaa gagcgctgac
    51901 aaagcgccgc tggcagctga agcgatgggt atcatcgctc cgcgtctgaa agaactaaaa
    51961 ctgatcgact ccatcatccc ggaaccgctg ggtggtgctc accgtaaccc ggaagcgatg
    52021 gcggcatcgt tgaaagcgca actgctggcg gatctggccg atctcgacgt gttaagcact
    52081 gaagatttaa aaaatcgtcg ttatcagcgc ctgatgagct acggttacgc gtaattcgca
    52141 aaagttctga aaaagggtca cttcggtggc ccttttttat cgccacggtt tgagcaggct
    52201 atgattaagg aaggattttc caggaggaac acatgaacat cattgccatt atgggaccgc
    52261 atggcgtctt ttataaagat gagcccatca aagaactgga gtcggcgctg gtggcgcaag
    52321 gctttcagat tatctggcca caaaacagcg ttgatttgct gaaatttatc gaacataacc
    52381 ctcgaatttg cggcgtgatt tttgactggg atgagtacag tctcgattta tgcagcgata
    52441 tcaatcaact taatgaatat ctcccgcttt atgccttcat caacacccac tcgacgatgg
    52501 atgtcagcgt gcaggatatg cggatggcgc tctggttttt tgaatatgcg ctggggcagg
    52561 cggaagatat cgccattcgt atgcgtcagt acaccgacga atatcttgat aacattacac
    52621 cgccgttcac gaaagccttg tttacctacg tcaaagagcg gaagtacacc ttttgtacgc
    52681 cggggcatat gggcggcacc gcatatcaaa aaagcccggt tggctgcctg ttttatgatt
    52741 ttttcggcgg gaataccctc aaggctgatg tctctatttc ggtcaccgag cttggttcgt
    52801 tgctcgacca caccgggcca cacctggaag cggaagagta catcgcgcgg acttttggcg
    52861 cggaacagag ttatatcgtt accaacggaa catcgacgtc gaacaaaatt gtgggtatgt
    52921 acgccgcgcc atccggcagt acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc
    52981 atctgttgat gatgaacgat gtagtgccag tctggctgaa accgacgcgt aatgcgttgg
    53041 ggattcttgg tgggatcccg cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg
    53101 ctgccaccac gcaggcacaa tggccggttc atgcggtgat taccaactcc acctatgatg
    53161 gcttgctcta caacaccgac tggatcaaac agacgctgga tgtcccgtcg atccacttcg
    53221 attccgcctg ggtgccgtac acccattttc atccgatcta ccaggggaaa agtggtatga
    53281 gcggcgagcg tgttgcggga aaagtgatct tcgaaacgca atcgacccac aaaatgctgg
    53341 cggcgttatc gcaggcttcg ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta
    53401 acgaagcctt tatgatgcat accaccacct cgcccagtta tcccattgtt gcttcggttg
    53461 agacggcggc ggcgatgctg cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg
    53521 agcgggcgct gcattttcgc aaagaggtcc agcggttgcg ggaagagtct gacggttggt
    53581 ttttcgatat ctggcaaccg ccgcaggtgg atgaagccga atgctggccc gttgcgctag
    53641 gcgaacagtg gcacggcttt aacgatgcgg atgccgatca tatgtttctc gatccggtta
    53701 aagtcactat tttgacaccg gggatggacg aacagggcaa tatgagcgag gaggggatcc
    53761 cggcggcgct ggtggcaaaa ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc
    53821 cttataacct gctgtttctc tttagtattg gtatcgataa aaccaaagca atgggattat
    53881 tgcgtgggtt gacggaattt aaacgctctt acgatctcaa cctgcggatc aaaaatatgc
    53941 tacccgatct ctatgctgaa gatcccgatt tctaccgcaa tatgcgtatt caggatctgg
    54001 cgcaagggat ccataagctg attcgtaaac acgatcttcc cggtttgatg ttgcgggcat
    54061 tcgatacttt gccggaaatg atcatgacgc cacatcaggc atggcagaga cagattaaag
    54121 gtgaagtaga aaccattgcg ctggaacaac tggtcggtag agtatcggca aatatgatcc
    54181 tgccttatcc accgggcgta ccgctgttga tgccaggaga aatgctgacc aaagagagcc
    54241 gcacggtact cgattttcta ctgatgcttt gttccgtagg gcaacattac cccggttttg
    54301 aaacggatat tcacggcgcg aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa
    54361 aaatggcggg ataacttgcc agagcggctt ccgggcgagt aacgtgctgt taacaaataa
    54421 aggagacgtt atgctgggtt taaaacaggt tcaccatatt gcgattattg cgacggatta
    54481 tgcggtgagc aaagctttct actgcgatat tcttggtttc acgctgcaaa gcgaagtcta
    54541 tcgcgaagcg cgcgactcgt ggaaagggga tttggcgctt aatgggcaat atgtgattga
    54601 gcttttctct tttccgttcc cgccggagcg ccccagccga ccggaagctt gcggcctgcg
    54661 tcatctggct tttagcgttg atgacatcga tgcggcagtg gcgcaccttg aaagccataa
    54721 cgtgaagtgt gaagccatcc gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa
    54781 cgatccggac gggctgccgt tggaactgta tgagcagtaa ggcttgtcat cgccgcattt
    54841 gcccggtaac gtgccgggca ttgctactgt aaaatcgcac catcatgaca ctcacgctca
    54901 atagacaact tctcacctca cgccagattc tggtggcctt tagcggcggg cttgactcca
    54961 ccgttctgct gcatcagttg gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg
    55021 ctattcatgt gcatcacggt ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa
    55081 acgtctgcca acagtggcag gtgccgctgg tggtcgaacg cgtgcaactt gcgcaagaag
    55141 gactgggcat tgaggcccag gcgcggcagg cgcgttatca ggcatttgcc cgcaccttgt
    55201 tgcccggtga agtgctggtc accgcgcaac atctcgacga tcaatgtgaa acttttctgc
    55261 tggcgctaaa acgcggtagc ggccctgccg ggctttcggc tatggcggaa gtttcggagt
    55321 ttgccggaac acggcttatc cgcccgttgc tcgcccgcac gcggggagaa ctggagcagt
    55381 gggcattggc gcatggttta cgctggattg aagacgaaag taatcaggac gactcatacg
    55441 atcgtaactt tctgcgcctg cgcgtagtgc cgttattgca gcagcgttgg ccgcattttg
    55501 cggaagcaac ggcccgcagc gccgcacttt gtgctgaaca agagagtctg ttggatgaac
    55561 tgctggcgga tgatttagca cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa
    55621 tgctggcgat gagtgatgcc cgccgcgcgg cgattatccg tcgctggctg gcagggcaga
    55681 atgcaccaat gccttcccgc gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc
    55741 gggaagatgc ctccccctgt ttacgcttgg gcgcgtttga aatccgccgc tatcagtcgc
    55801 aactgtggtg gattaaatcc gtcaccgggc aaagtgaaac cattgtgccg tggcagacgt
    55861 ggctacaacc gctggaatta ccggcggggc tgggaagcgt acagcttact gcgggaggcg
    55921 atattcgccc tccgcgtgca gacgaagcgg tcagcgtgcg tttcaaagcg gcaggattgc
    55981 tgcatattgt cgggcgcaac ggcggacgta agctgaagaa aatctggcaa gagttaggcg
    56041 tgccgccgtg gctacgtgac accacgccac tgctgtttta tggcgaaacg ctgattgcgg
    56101 cggcaggggt atttgtgacg caagaaggtg tggctgaagg tgagaatggc gtaagttttg
    56161 tctggcagaa aacgcttagt taagtgaaag ccggataaga cgcatcaaac gtcgcatccg
    56221 gcgaaagtca atcaggactc gctcaccacc accgtaccga tttccgggtg gctaaagctg
    56281 gtaattttat ccagacgtag ctcacgggtt tcgccagcgg cctcgacgac cagatactcc
    56341 acatttttgc gggagactaa atcgctggct tttgcctgca atttttcgcc atctttcagc
    56401 tcaagtgtca gcattaaatg atgctggcag gcgagctcaa gattatcgta atcatcacaa
    56461 ttgattggtt gatacgtatc attcattgac ataatcgctc accagtaagt ttgccgcagc
    56521 gtatgctgct ttttccctga cagcctcaga aagggcgtcg tcggcagcca tttcattcag
    56581 cactttcaaa acgcagccca gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc
    56641 cgcgtaccgc ttgcgtatta actcacaata tttttccaca tgccctcctg tcagcactct
    56701 gacttaaccg tggatgcaag tctaagccta cgaagataaa ctctgtttcg caaggtgact
    56761 ataccacact catgtctgca atatcagcgc cgcaactgca cgtattccgt tacaatggcc
    56821 tcctgattcg aaaggagttt tcttatggcg cttaaagcga caatttataa agcgacggtt
    56881 aatgtggccg atctcgaccg caaccagttt ctcgatgcct ctctgacgct ggcgcgccac
    56941 ccttcagaaa cccaggagcg tatgatgctg cgcttgctgg cgtggctgaa atatgctgat
    57001 gaacgtctgc aatttacccg tggtttgtgt gccgatgatg agccggaagc gtggctgcgt
    57061 aacgatcacc tgggcattga tttgtggatt gagctggggc tgccggatga gcggcggatt
    57121 aagaaagcct gcacccaggc cgcagaagtg gcgctgttta cctataatag tcgggcggcg
    57181 caaatctggt ggcagcaaaa tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg
    57241 tatctggacg atgaacaact ggcgaaagta agcgactttg ccgatcgtac catgacgctg
    57301 caggcaacga ttcaggatgg cgtgatctgg ttatcggacg ataagaataa tctggaagtt
    57361 aacttaaccg cctggcaaca accttcatga ttgtgatttc ccgacatgtt gctattcccg
    57421 atggtgagct tgagatcacc gccattcgtg cgcagggcgc gggcgggcag catgttaata
    57481 agacctcaac ggccattcat ctgcgttttg acattcgggc gtccagcctg ccagagtatt
    57541 acaaagagcg tctactcgcc gccagccatc atttgatcag cagtgatggc gtgattgtca
    57601 ttaaggcaca ggaataccgc agtcaggaac tgaaccgcga agccgcgcta gcccggctgg
    57661 tggcagtgat taaagattta acaacagaac aaaaagcccg acgacccacg cggcccaccc
    57721 gtgcatcgaa agagcgcagg ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc
    57781 gcggcaaagt acgcagcggt cgggaataaa aagaaggaat agatggtgaa aaaagcgata
    57841 gtgacagcga tggctgtaat cagcctcttt actctgatgg gatgtaataa ccgggccgaa
    57901 gtcgatacgc tttctccagc acaggctgcc gaactgaaac cgatgccgca aagttggcgc
    57961 ggcgtgctgc cgtgtgctga ttgcgaagga atcgaaacct ctctgttcct cgaaaaagac
    58021 ggaacatggg tgatgaatga gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct
    58081 tcctacggta catgggcgcg aaccgctgac aagctggtat taaccgacag caaaggtgaa
    58141 aagtcatatt atcgggcgaa aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg
    58201 attgaatcgc agttcaacta tacgctggaa ccggcacaat ctagtttacc tatgacgccg
    58261 atgaccctgc gaggcatgta tttttatatg gctgatgcgg cgaccttcac tgattgcgcg
    58321 accggaaaac gtttcatggt agcgaataac gcagagctgg agcgtagcta cctggctgcg
    58381 cgcggtcaca gtgaaaaacc ggtgttactg tcagtagaag gtcactttac gcttgaggct
    58441 aatccggata ctggtgcgcc aaccaaagta ttagcgcccg atacggcggg taaattttac
    58501 ccgaataagg attgcagtag tttggggcag taaacctgct taaggcagaa acaaacgcaa
//
LOCUS       DAZFKM010000014        57578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFKM010000014 DAZFKM010000000
VERSION     DAZFKM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..400
                     /gene="tnpA"
                     /locus_tag="ACHKAY_000956"
                     /pseudo
     CDS             1..400
                     /gene="tnpA"
                     /locus_tag="ACHKAY_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(499..1167)
                     /gene="ydcL"
                     /locus_tag="ACHKAY_000957"
     CDS             complement(499..1167)
                     /gene="ydcL"
                     /locus_tag="ACHKAY_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO5699672.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1470..2063)
                     /gene="tehB"
                     /locus_tag="ACHKAY_000958"
     CDS             complement(1470..2063)
                     /gene="tehB"
                     /locus_tag="ACHKAY_000958"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO5699673.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2060..3052)
                     /gene="tehA"
                     /locus_tag="ACHKAY_000959"
     CDS             complement(2060..3052)
                     /gene="tehA"
                     /locus_tag="ACHKAY_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO5699674.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3176..4156
                     /gene="ydcK"
                     /locus_tag="ACHKAY_000960"
     CDS             3176..4156
                     /gene="ydcK"
                     /locus_tag="ACHKAY_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO5699675.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4151..4687)
                     /gene="rimL"
                     /locus_tag="ACHKAY_000961"
     CDS             complement(4151..4687)
                     /gene="rimL"
                     /locus_tag="ACHKAY_000961"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO5699676.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4750..4974)
                     /gene="ydcH"
                     /locus_tag="ACHKAY_000962"
     CDS             complement(4750..4974)
                     /gene="ydcH"
                     /locus_tag="ACHKAY_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO5699677.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5114..6769)
                     /gene="opgD"
                     /locus_tag="ACHKAY_000963"
                     /gene_synonym="mdoD"
     CDS             complement(5114..6769)
                     /gene="opgD"
                     /locus_tag="ACHKAY_000963"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO5699678.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6994..8337)
                     /gene="ydcJ"
                     /locus_tag="ACHKAY_000964"
     CDS             complement(6994..8337)
                     /gene="ydcJ"
                     /locus_tag="ACHKAY_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5699679.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8554..9477
                     /gene="ydcI"
                     /locus_tag="ACHKAY_000965"
     CDS             8554..9477
                     /gene="ydcI"
                     /locus_tag="ACHKAY_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5699680.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9515..11155)
                     /gene="trg"
                     /locus_tag="ACHKAY_000966"
     CDS             complement(9515..11155)
                     /gene="trg"
                     /locus_tag="ACHKAY_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO5699681.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11554..11703
                     /gene="hokB"
                     /locus_tag="ACHKAY_000967"
     CDS             11554..11703
                     /gene="hokB"
                     /locus_tag="ACHKAY_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO5699682.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11681..11749)
                     /gene="yncP"
                     /locus_tag="ACHKAY_000968"
     CDS             complement(11681..11749)
                     /gene="yncP"
                     /locus_tag="ACHKAY_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO5699683.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(11775..11948)
                     /locus_tag="ACHKAY_000969"
     CDS             complement(11775..11948)
                     /locus_tag="ACHKAY_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699684.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12193..12723)
                     /gene="cybB"
                     /locus_tag="ACHKAY_000970"
     CDS             complement(12193..12723)
                     /gene="cybB"
                     /locus_tag="ACHKAY_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO5699685.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12912..13913
                     /gene="gap"
                     /locus_tag="ACHKAY_000971"
     CDS             12912..13913
                     /gene="gap"
                     /locus_tag="ACHKAY_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5699686.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13955..15394)
                     /gene="aldA"
                     /locus_tag="ACHKAY_000972"
     CDS             complement(13955..15394)
                     /gene="aldA"
                     /locus_tag="ACHKAY_000972"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO5699687.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15591..16391)
                     /gene="ydcF"
                     /locus_tag="ACHKAY_000973"
     CDS             complement(15591..16391)
                     /gene="ydcF"
                     /locus_tag="ACHKAY_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO5699688.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16663..20565)
                     /gene="hrpA"
                     /locus_tag="ACHKAY_000974"
     CDS             complement(16663..20565)
                     /gene="hrpA"
                     /locus_tag="ACHKAY_000974"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO5699689.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20766..21371
                     /gene="azoR"
                     /locus_tag="ACHKAY_000975"
     CDS             20766..21371
                     /gene="azoR"
                     /locus_tag="ACHKAY_000975"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO5699690.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21422..22738)
                     /gene="ynbD"
                     /locus_tag="ACHKAY_000976"
     CDS             complement(21422..22738)
                     /gene="ynbD"
                     /locus_tag="ACHKAY_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO5699691.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(22728..24485)
                     /gene="ynbC"
                     /locus_tag="ACHKAY_000977"
     CDS             complement(22728..24485)
                     /gene="ynbC"
                     /locus_tag="ACHKAY_000977"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO5699692.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGSRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24501..25397)
                     /gene="ynbB"
                     /locus_tag="ACHKAY_000978"
     CDS             complement(24501..25397)
                     /gene="ynbB"
                     /locus_tag="ACHKAY_000978"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO5699693.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25397..26002)
                     /gene="ynbA"
                     /locus_tag="ACHKAY_000979"
     CDS             complement(25397..26002)
                     /gene="ynbA"
                     /locus_tag="ACHKAY_000979"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5699694.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(26173..28479)
                     /gene="ydbD"
                     /locus_tag="ACHKAY_000980"
     CDS             complement(26173..28479)
                     /gene="ydbD"
                     /locus_tag="ACHKAY_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO5699695.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28543..29403)
                     /gene="pdxI"
                     /locus_tag="ACHKAY_000981"
     CDS             complement(28543..29403)
                     /gene="pdxI"
                     /locus_tag="ACHKAY_000981"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO5699696.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29634..30224)
                     /gene="paaY"
                     /locus_tag="ACHKAY_000982"
     CDS             complement(29634..30224)
                     /gene="paaY"
                     /locus_tag="ACHKAY_000982"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO5699697.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(30206..31156)
                     /gene="paaX"
                     /locus_tag="ACHKAY_000983"
     CDS             complement(30206..31156)
                     /gene="paaX"
                     /locus_tag="ACHKAY_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO5699698.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31257..32570)
                     /gene="paaK"
                     /locus_tag="ACHKAY_000984"
     CDS             complement(31257..32570)
                     /gene="paaK"
                     /locus_tag="ACHKAY_000984"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO5699699.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32597..33802)
                     /gene="paaJ"
                     /locus_tag="ACHKAY_000985"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32597..33802)
                     /gene="paaJ"
                     /locus_tag="ACHKAY_000985"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO5699700.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELYRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(33802..34224)
                     /gene="paaI"
                     /locus_tag="ACHKAY_000986"
     CDS             complement(33802..34224)
                     /gene="paaI"
                     /locus_tag="ACHKAY_000986"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO5699701.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34214..35641)
                     /gene="paaH"
                     /locus_tag="ACHKAY_000987"
     CDS             complement(34214..35641)
                     /gene="paaH"
                     /locus_tag="ACHKAY_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO5699702.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35643..36431)
                     /gene="paaG"
                     /locus_tag="ACHKAY_000988"
     CDS             complement(35643..36431)
                     /gene="paaG"
                     /locus_tag="ACHKAY_000988"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO5699703.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36431..37198)
                     /gene="paaF"
                     /locus_tag="ACHKAY_000989"
     CDS             complement(36431..37198)
                     /gene="paaF"
                     /locus_tag="ACHKAY_000989"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO5699704.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37195..38265)
                     /gene="paaE"
                     /locus_tag="ACHKAY_000990"
     CDS             complement(37195..38265)
                     /gene="paaE"
                     /locus_tag="ACHKAY_000990"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO5699705.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38273..38770)
                     /gene="paaD"
                     /locus_tag="ACHKAY_000991"
     CDS             complement(38273..38770)
                     /gene="paaD"
                     /locus_tag="ACHKAY_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO5699706.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(38785..39531)
                     /gene="paaC"
                     /locus_tag="ACHKAY_000992"
     CDS             complement(38785..39531)
                     /gene="paaC"
                     /locus_tag="ACHKAY_000992"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO5699707.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39540..39827)
                     /gene="paaB"
                     /locus_tag="ACHKAY_000993"
     CDS             complement(39540..39827)
                     /gene="paaB"
                     /locus_tag="ACHKAY_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO5699708.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(39839..40768)
                     /gene="paaA"
                     /locus_tag="ACHKAY_000994"
     CDS             complement(39839..40768)
                     /gene="paaA"
                     /locus_tag="ACHKAY_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO5699709.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41053..43098
                     /gene="paaZ"
                     /locus_tag="ACHKAY_000995"
     CDS             41053..43098
                     /gene="paaZ"
                     /locus_tag="ACHKAY_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO5699710.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43346..45619
                     /gene="tynA"
                     /locus_tag="ACHKAY_000996"
     CDS             43346..45619
                     /gene="tynA"
                     /locus_tag="ACHKAY_000996"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO5699711.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45677..47176)
                     /gene="feaB"
                     /locus_tag="ACHKAY_000997"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(45677..47176)
                     /gene="feaB"
                     /locus_tag="ACHKAY_000997"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO5699712.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47412..48317
                     /gene="feaR"
                     /locus_tag="ACHKAY_000998"
     CDS             47412..48317
                     /gene="feaR"
                     /locus_tag="ACHKAY_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO5699713.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48489..48818)
                     /gene="ydbL"
                     /locus_tag="ACHKAY_000999"
     CDS             complement(48489..48818)
                     /gene="ydbL"
                     /locus_tag="ACHKAY_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO5699714.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(48823..49008)
                     /gene="ynbE"
                     /locus_tag="ACHKAY_001000"
     CDS             complement(48823..49008)
                     /gene="ynbE"
                     /locus_tag="ACHKAY_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO5699715.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49005..51644)
                     /gene="ydbH"
                     /locus_tag="ACHKAY_001001"
     CDS             complement(49005..51644)
                     /gene="ydbH"
                     /locus_tag="ACHKAY_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO5699716.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            51852..52841
                     /gene="ldhA"
                     /locus_tag="ACHKAY_001002"
     CDS             51852..52841
                     /gene="ldhA"
                     /locus_tag="ACHKAY_001002"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5699717.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            52952..53374
                     /gene="hslJ"
                     /locus_tag="ACHKAY_001003"
     CDS             52952..53374
                     /gene="hslJ"
                     /locus_tag="ACHKAY_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO5699718.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53371..53637)
                     /gene="ydbJ"
                     /locus_tag="ACHKAY_001004"
     CDS             complement(53371..53637)
                     /gene="ydbJ"
                     /locus_tag="ACHKAY_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO5699719.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            53911..57435
                     /gene="nifJ"
                     /locus_tag="ACHKAY_001005"
     CDS             53911..57435
                     /gene="nifJ"
                     /locus_tag="ACHKAY_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO5699720.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14552 a        15133 c        13717 g        14176 t
ORIGIN      
        1 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
       61 tttgtcacca gataccgacg ccagattttt tgacgcgaca gaaaaactac gcacttactt
      121 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      181 cgtccatttg ttaattaact aacctcccaa actggcgata tccagtctgg taaacagcct
      241 caaaggcgta tcgggtaggt tactgcgacg agatcgacca gatattgcag tcaggtatta
      301 ctacaaaggc gttttgtgga gtcctggcta ttttgccagt agctgcggag gtgcgccaat
      361 atccgtcatc cgccaataca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcac
      421 cttatatccc cgccctgaag gatggggttt tacggcacac cggataaaaa gcgccatcta
      481 acgatggcgc gtctggaact actttttgtt aacgtcaaac atggtggcat cggtcgccat
      541 gtcatcaata acttgtttaa tattttcgaa agccattggc gtactttcgt tattcaggtc
      601 tttaccttcg ccctgacgaa cgactttgat aaccggttta ttagtcgctg catcaatcag
      661 ctcaccttca aaatagaggc gagtatccat ggtacggtgg cctgtagcca tttgcgtccc
      721 cgcaaccact aatgcaacag gaaccacttc atagaattgc agcccttctt tgctggtatc
      781 tacaccggta atggccccac ggaaaatcag actacgcggc ccagcggtgg taaccagtgg
      841 tttacgctgc gctatcgctt ctttcatttc ggtgttggta tagttcaaaa ttttatccag
      901 aactttctgc cctacctggg tagaaggttt tggcaccgga taataagtga ttgggttcca
      961 gacgatgctg tcatatttgc tttgatcaaa actcgggtct acccaacgta aaacaggttt
     1021 acctgttgct gaggttgttt cttttaaatc agagtaattg tttaaaaaac cagaatattt
     1081 atctggctgg gtgattttag aggcacaacc agacagagcc aacaagccgc ataaagctgc
     1141 aactttcgca aatgatgtgg tacgcatgaa tagtttcctg agatgattat attacttttt
     1201 aagttatagc aaaaagcgcc gcttcgtgtt gtggcaaaaa aagtgttggt gaaaataaat
     1261 ttttcagccc cttttcagac gacatagtca atgccgaaaa aggggctaag tcaatcatca
     1321 gtagttaaat ttcagtaagt taagctgcta aaaaacttcg actactgtga ttaatttcac
     1381 atcccttcga taaaaaatat gcttaccgaa aagaagaaca gtaaatcatc tcagcgtggg
     1441 gaattttcac gcgccttatt cttagcgggt cattttttac gtgccagcat cgtggcgaaa
     1501 cgcagtttaa tacgattacc gttggcgtcg gtgcggtgca gctcgccgac gtcttcattg
     1561 tatttcaccc tctcccagcc ttcgtaatat cgacgtaatt ctccctcttt gaaggcaaac
     1621 gggaagccga cggtacatgg ataatcagcg gtatccatcg ccgccacaat caggttgtaa
     1681 ccaccaggtt tagtgcaacg ttgcatattg gcaatcagcc ctgggatggt tttagcctca
     1741 aggaacatca gtaccacagt cgaaagaata aaatcgtact gtctatcaaa tgtgaggtta
     1801 ttcagatcaa cgactcgggt gtgtaaatta tccagatttt caatggattt aatgcgctct
     1861 acattggcga tactcatggc atttttatcc catgcgtcaa catcataacc attggctgcc
     1921 aggtaaagac tgttacgacc attgccacag cccagatcca gcgttttacc cggtttaacc
     1981 actttcaccg cttccagtac ttcagagtgt gtgcgggtta attcatattt atcagtaaaa
     2041 tagttttcgt cacgaatgat cattctttgt cctctgcttt cattaaaacg gcgcgctcgg
     2101 ttctgactaa caattttccc tgcatcagaa gcgcaaaagt acggatgagc agtattgcaa
     2161 taataaaatt ggtaaagata aacagcggta ccgccagcgt atggaaaaat ccattttcgc
     2221 tgccactccc cagatgcaaa ccggtggttg ccagtgcaga tacgccgaac gagaaactcc
     2281 agaatgaagc attaaatggc tgggagagat accatggcat cagacgtagc ataaacagca
     2341 gttgcagcag tccataacca aaaagcattt tcgccagcgt atcaccctcg ccgccgttga
     2401 cgctcaacca ggcactacag gccaccagcg caggagcgag ctgaatgccg agtgatgtcc
     2461 gcagtgccgt gggtaattct cccgaactgc gcagacgctg caagatcaca ggttcaaggc
     2521 ttagccatga gaaaacgcct gcgcctaaaa acaccagacc ggcgtcggtg tagcccaacg
     2581 caccacaggc cattgcgctg ataaagttgt tggcaactgt cggcagatac agtccaggcg
     2641 tggtagcttc ttcagggtga gatccgcgcc ataatcccgc agtttgccag gcggcataag
     2701 ccaactgaac cacgacacca aaactgaaca ggcacaccgc cagtgggcga aaccacggaa
     2761 caaaaccaat cgccaccagc atcgtcgttg ccggaaacaa actcacaaaa ctgctcagca
     2821 ctggatggcg aacttccgcc agcacgctat gcggaaagcg tatgagtcgg gtaataaatg
     2881 cgctagttaa taatccccag atgatcatcg ccagaatcac cagcccatcc cctaaccagt
     2941 ggctgacctg ccaaacctgg ctggcatagc gccaggcaaa tcccatccct atcgtcccca
     3001 acacaatacc aaagtagcct gccggcaaat tgagcacttt atcgctctgc atcttcttac
     3061 tcattttgtt taatttataa agtatatttg aaatgcattt tatggaattt tctcgagcat
     3121 agccagagcc gcagaatttg ctacggttat agtaagtcac ctgtgggaaa acgctatgcg
     3181 caaatatcgt ctaagtgaag aacagcgagc ctttagttat caggaagatg gcactaaaaa
     3241 aaatgtgtta ttacggcaga ttatcgccat tagcgatttt aacgatgtga tagcaggaac
     3301 cgctggcggc tggatcgatc gcgaaacggt gttagcacag gaaggaaatt gctggattta
     3361 cgaccagaat gctattgcgt tcggcggaac ggtgatttcc ggtaatacgc gcatcaccgg
     3421 caccagcgta ctgtggggag aggtttacgc aacggataat gtctggatcg ataatagtga
     3481 aatcagccag ggtgcatata taagtgacgg tgtcaccatt catgactcac tggtttgcgg
     3541 gcaatgtaga atatttggtc acgccttaat taatcaacat tctatgatcg tcgcagcaca
     3601 aggcttaacg cctgaccatc aactcctgct acaaatttat gatcgcgcaa gggttagcgc
     3661 ctcgcgtatt gtccaccagg cacaaattta tggcgatgcc gtcatccggt atgcttttat
     3721 tgaacatcgt gccgaagttt ttgattttgc cagtattgaa ggtaatgaag agaacaacgt
     3781 ctggctatgc gattgcgcta aagtctacgg tcacgcgcag gtgaaagctg gcatagaaga
     3841 agatgccatt ccaacgattc attacagttc gcaggtggcg gaatatgcca ttgtggaagg
     3901 taattgtgtg ttaaaacatc atgtcttgat tggcggaaat gccgtggtac gtggtggacc
     3961 gattctgctc gacgagcacg ttgtaataca aggtgagagc cgtattacag gtgcggtgat
     4021 aattgaaaac catgtggagt taaccgacca cgccgtcgtt gaagcctttg atggcgacac
     4081 ggttcatgtc cgtggtccaa aagtcattaa cggcgaggag cgcattacgc gaacgccgct
     4141 ggcagggtta ttatgaatcg ataatacgcg cgtataagtt cacatcatca taggcatcat
     4201 tcaggaactc agcctgtttc aggcaacctt caaggataaa accattgcgc aaagcgacct
     4261 ggttgctttg cggattgtcc acccgacatt tgatcacgaa gcgtctaagt tcaccagact
     4321 gggcgtaatg atgaatcaat gcctgcagcg cctgagaaat gatcccctgc ccctgatgag
     4381 attcgtccag ccagtagcct atttcagcgg ttttattcag tggttcaata cgattaaacg
     4441 agataacgcc gataagttca tcttctttga aaatcatgaa cattttggca tagccgcgtt
     4501 gatgcaacat cacattaccc tgcaccgttt ttcgcgtgtc ctcttcactc tgaacgaact
     4561 gcggccagtt tagcgactgc tgtaaccagg ttttattttt acagattaac tgataaagag
     4621 gagtgacgtg attttctgca acagcatgta attcaagtga ttcgcttact tttatcgttt
     4681 cagtcatctt atctccagtc aatccatgca gttgttaaaa aagccgcctg gctggcggct
     4741 taggaaagtt tacacctctt tgacgctctc ctgctgcagg attttgagca tctcatcttt
     4801 taactgtagc ttttgttttt tcatgcggac cacttccgca ttgtaccctc gaccgtcgga
     4861 accttccttt ctggcaattt catgatcaag tttattgtgt ttatcgaaca aggacataaa
     4921 gcgaggattt tcgtttttca gacgggatat taaatctcga tattctggaa acatacgcac
     4981 tccctgttag tggctgacaa ggtgtatctg aaatacacac catcagatta ctcatttaac
     5041 ttagggaaaa atgcgagcgg gatcgtgttt aaccagacgg cacctcccga agaaggtgcc
     5101 gaagaaaaaa cgattaactc atcacgcggt cgtcaacata ctgacgttta tccggcgctg
     5161 gcgggaaata ctgatacagc catgtttcac tgatagcgtc gccctgacaa cgcagataca
     5221 tccgcatatc gaccggatca gtggagtccg aagtcggata ccagtcaaac tgaatacgat
     5281 aaccatcaat gggttcaata tagagaattt cgatttgctt cgcttcccca ctggaaagcg
     5341 taatcaccgg ctcaatgcct tttggtgcgg cagctttcag atcaccacca acgaaatcga
     5401 cggcaaaacg acgcgcccat ttttcgggat agtgttcacc tggtgcccaa ccttccggga
     5461 aaccgcccat gccggtacgc gtcgccataa cgcgcgctaa tgggcaatga acaggcggtt
     5521 gcgcactcca gtacagacga tactggaatg caaactcatc acccgccttt acagcttttt
     5581 ctggctgcca gaagcagaca atgttatcca gcgtttcgcc cgttgttggg atttccatca
     5641 ggccgatggt gcccttaccc cacttgttac gcggttccac ccacagactt gggcgtttgt
     5701 tataccagcc cataatgtcc tgataatggg agaaatcacg atccagttgc aataaaccaa
     5761 accctttcgg gttgttgtcg gtgtaagcat tgaactgcaa tttttgcgga ttattcagcg
     5821 gacggcaaat ccactcgccg ttgccccgcc acatggacag acgatcagag tcatgaattt
     5881 gcggatgaat ggtgtcgcac atccgacgtt cattagtacc gcagctgaac atactggtca
     5941 tcggcgcaat gcccagctgt ttaatgtctt tgcgcgcata caggtgattt tccacatcca
     6001 taatcacctg acttttctca caatggatag tgaacttata ggcaccagta atgctggcgc
     6061 tatcgagcaa cgcataaacg gtaaaggtag ttgcccccgg ttttaccgta tcaaaccaga
     6121 aggcggtaaa gtcggggaac tcttctttac tgtcggtgta agtgtcaatc gccaggccgc
     6181 gggccgacaa accgtattga tatgtgtcat caacggcgcg gaaataactc gctccgagga
     6241 atgatactac atcacggcgc gccagttcgg gggctttaaa cacgcgaaaa ccggcaaagc
     6301 cgagatcgct ttgcccttct aattgtttgg tatcaacacc tgcatcgttg tatttgaaca
     6361 actccgggcg aaagtgaatt tcacgcgcca gatgtgttgc tggatctaca gaaaacatac
     6421 gaacgcggcg acggaatccc attcccatat ggaagaactg agcgtccagt tgacggttct
     6481 caacgttatg ccagagcgat ttttcggcgt cgtattgaat actgttataa gcctgcggcg
     6541 tcattgtcgc cagcgtgtca ggtaacggac gaggcgcacc acgccacgct gtttgcgcta
     6601 agtcgtgcgc cattgactgt agaatggaga agtcaaaacg ctgggtttgc ccgtcggcaa
     6661 tatcagaatc tgccgcgaat gccgcctgag aaaaaagaga agcaatgccg ctggtaccgc
     6721 acacggcggc catagccatt gaacctttaa taaatcgtct acgatccata cctgaaagtg
     6781 agtccttctg gtgttgtgaa tggtcccgcg ctggtcgttt tcagcggcaa gaaagtgctt
     6841 atttatgacc gctcactcta gacaatattt attaataatc caattgttga actcagagaa
     6901 tatgcataaa tcggcaattt ttttatgtcg atgggatgag cctgattcgt ttgtaaaatc
     6961 aggcaaattt ttgcaaaggc ggatggtcag aatttaaagc aaaccacagc gacgtttact
     7021 gcgttcttcc gcttcctgat aaagctggaa ctcatccaaa accggacaac ccaacgcctg
     7081 ctcaaatgcc tcgcgactgg cattaccgtg attgcgtgcc tgcgtttcat tacccagatt
     7141 tgactggaag atccccgccg cgctaacggg caagaaatct tcataggtaa tgggttgcgc
     7201 cgcgacccaa ccacgttcaa ttaagggctg tggatcgtct ccgggatgaa tcgcctgacg
     7261 atgcgcctca cctgaaggcg tcagacggta ccggaaccat gccagccctt gctgacgcat
     7321 taaaaactca ctgtcaggaa aagtgcggaa ggtttcctgt aaatgcattt ggtgagtgag
     7381 attatcctgc ccggttccag cgttacgcag aagatcatca tacagttgtc gccctttcgg
     7441 cgttaatgcc acgccacgct gctcaatttc accaaagcgc gcggtatgcg tgccctgttt
     7501 ctgccccgca aacaacaccg tctcttccag tgctttaaag ctggtctggc gtagtaaaat
     7561 cggtacctcg cggcgcggcg gcccctcgat gagaattttg ggttcaattc cgcattcagg
     7621 catcatcgac tgcacccggt caatatccag cgtacgtggc gtcaggtggt tgatatggca
     7681 tccaggaaaa cagaccacat cagcaatcaa ccgatgttcg ttgtgcaagg cgcgataggt
     7741 ttcttcatct accgttgctg actggtgcca gcgaaacgtt tccagggctt cctgcacaaa
     7801 ctcctgtgcc tgtgtttcgt taaaaccgcc ctgctgctca tattcctcta acagttgtcg
     7861 acaacgtggg gtgaagatat cgcgctgacg cagaatctcc gccgctttct ggcgcaaaat
     7921 ttcgttctcg ataagctcaa ggcggagtaa ggaggtaaaa acgcggaagg gattacgcgc
     7981 cagagaagca tcatcaatgg gccgaaatgc tgtcgaatgt accggcaccc ctgcctgcga
     8041 gagatcgtaa tagctcaccg ggtacatccc cataatggca aacatccgcc gaagagtagc
     8101 aagctcttgt gcagtcccaa cgcgaatcgc cccatgacgt tcaacattca gtcgcgccag
     8161 ctcgtctgca tttaccattt tttcgtgcag ttgaggattg ttttccagca cagccagatt
     8221 cacatcagct accagttcca gcagcgtgcc atattgcgga acttcttgct ggtacatggc
     8281 tgacattgcc tgcgaaaact gttcccgaat ctcatccgcc gtgatgctgt tcgccatagt
     8341 gtctgcctcc agtggtcagt aatctggagt gtaggtaacc gcattcactc tggcgggaag
     8401 aatttacaaa ctgtgatctc gccgcgaaaa catcaatatt atccattttg ctgtaacaca
     8461 attgctttaa ttgttaataa tattttgcaa tcaagttatc ataatcaaac aacttcactt
     8521 gtcagcgaca ccgcttcgtt tttaacatcg cttatggaaa aaaatagtct gtttagtcag
     8581 cgcatccgtt tgcgccacct tcatacattc gtagctgtcg cacaacaagg aactttgggg
     8641 cgcgcggctg aaacccttaa tttgagtcaa cctgcgctct ctaagacatt gaatgaactg
     8701 gagcagctga ctggcgctcg cttgtttgag cgtggtcgtc agggggcgca acttacctta
     8761 cccggcgaac aatttttaac gcatgcagtc agagttcttg acgccatcaa cactgccgga
     8821 cagtcgcttc atcgtaaaga aggtcttaat aatgatgtcg tcagggttgg tgcactacct
     8881 actgcggcac tggggatatt accttcggtt ataggtcagt ttcatcagca acaaaaagag
     8941 acgaccttgc aagttgcgac aatgagtaac cctatgattc tggcgggttt aaaaaccggg
     9001 gaaatcgata tcggcattgg tcggatgtca gatcctgaac tgatgaccgg gcttaattac
     9061 gaactgctgt ttcttgaatc gttgaagctg gttgtccgcc ctaatcaccc gctacttcag
     9121 gagaacgtaa cgctaagccg ggtgctggaa tggccggtcg ttgtatcacc agaaggcact
     9181 gcgccacgcc agcattcaga tgcattagtg cagagccagg gctgtaaaat tccttcgggt
     9241 tgtatcgaaa cgctgtctgc ttcgctatct cgtcaactta cggttgaata cgattacgtg
     9301 tggtttgtcc cttctggcgc tgtaaaagac gacctgcgtc atgccacgct ggtggccctg
     9361 cctgttccgg gacatggtgc aggcgaaccg attggaatac tgacccgcgt agatgcgacg
     9421 ttctcttctg gttgccagtt gatgattaac gctattcgaa aatcaatgcc gttctgaaag
     9481 gtgaagggat ctgtcgatcc ctccttgaac attttcacac cgtagcgaaa ctaactggtt
     9541 cacccgctcc gcgaggttct gccgacacag aatgtttgtg cagacggaat acatccaccg
     9601 cctcagttaa tcgtgccgcc tgttcttcaa gggacaccgc cgccgctgag gcctcttcta
     9661 ccagagaagc attctgttgc gtcaccttat ccatttcaga aatcgcctgg ctaacctgcg
     9721 ttatgcctct actttgttca tccgaggcgg cggcgatttc ctgcatgata tgtgtgacac
     9781 tcgcgacggc atcaacaata gtgctcatgg ttttccctgc cgttgccacc tcatccgacc
     9841 ccaggtcaat taacctgact gattcactga tcaagccttc aatctctttc gccgcttggg
     9901 cgctgcggct tgcgagtgtc cgtacttcgc tggcgacaac ggcaaatcca cggccttgct
     9961 cacccgctcg cgcggcttca acggcagcat tcagtgccag aatattcgtc tggaaagcaa
    10021 tactgttgat gacggcggtg atctcggaaa ttttcttcga acttgtagag atagcgccca
    10081 tcgtttttac tacaccggaa accgtctgcc cgccatcgct ggctttaata gaagcctctt
    10141 gcgccagttt gctggcatga tgcgcgttat ccgcattctg tttcaccgtc gcagtgagtt
    10201 gctccatgct agcggcagtt tgttcgatag ccgccgcttg ttcttcggtg cgagatgaca
    10261 ggtccgcatt gccagctgaa atttcgctgg tgccacgata aatctcttcc gcaccctgtc
    10321 gaacagtccc tactgtcatc cccagtgaat gctgcatttg ctgtaaatga cgacttaagc
    10381 gaccgatttc attacgaccc gccggttcat cattcatcgt cagatcgcca ctggcgattt
    10441 tttcaatccg ttgtgcggca tgttgcagtg gacgaatgac gatccgacgt agcaccataa
    10501 atgttatcag cgtcatgacc agggcaagca caaacgcgcc aatcatgaac atcccaccca
    10561 ggcgggtgcg ctgatgggcc agttccgcca gttggttggc tctggtgcta cgtatcttaa
    10621 cggctttgtt caaaatatcg gtataagcat tatccagcgg tcggatctgc tcactttcat
    10681 gattgataat cgcttcaaac atgccatttt tggcatattt caacataggt tgcatacccg
    10741 tgatataagc ctgaaagcgt tgatttaatt cagtgtcgag ggcttcatca gcaggtgttt
    10801 tcaccggtcg attctgataa gcacgataac cttgctgcga ctgtttaatc tccgattcgg
    10861 cttgcgcaat atttcgcttc attgcttcca tttccgcaat acgacttgca gccccggcct
    10921 gaatcatatt gatccgggct gaacgcaaaa aatctgaact gttcgctaac ccggtccgaa
    10981 tctcaatctc cgcagtaaca tcacgctgat cgcgatcggc ctgccagagg aaatagccag
    11041 ccagagcaga acttaatgcg aatagaacta agataccgcc aagaatgcag gcaaataacg
    11101 gaaccagcct gatgtgatgc aaaaaaccta atcgatgtga gggagttgta ttcattaccg
    11161 ttgtctctcg tccaggttta ctcctgatag aaagtcatcg gcacttgacg gtaattactt
    11221 atgaccaaaa acctctttgc gtcacatttt tacaacaatt attgcagaga aaattaatgc
    11281 tattggaatg aagagagtaa cgaatggcta aactggctaa cccgaatgac gaaaaattct
    11341 ggaaaacagc acgcaatttc gtgaagtgat tattttcgtc cctaaggaaa cctcatagga
    11401 tgcctgcggg tgcctgagac tttctgtttt caggctaacc gggaatcaga aagacgaagg
    11461 ccccgaccga tatttctatc aaccgaggcc aacgaatgaa cctagcaatt cagatcttag
    11521 cctcttaccc gccatccggc aaggagaaag gctatgaagc acaaccctct ggtggtgtgt
    11581 ctgctcatta tctgcattac gattctgaca ttcacactcc tgacccgaca aacgctctac
    11641 gaactgcggt tccgggacgg tgataaggag gttgctgcgc tcatggcctg cacgtccagg
    11701 taagggcaag cgcggggatt ttccccgcgc atctgactag cagattcatt ttctcatggc
    11761 acccctttta cacttcagtt agtgcatgtt tttttcgagg cactaataga accatcatta
    11821 cagacaaatt tgccgcctgc cgtacagtgt gagatccccc ctttcgaacc agaacagggt
    11881 ttgcgcccgg catcggcata aaaggaaaca agcgttccca taaacaaaat aagtgccagt
    11941 tttttcatat aacctccatg gtttatcgaa tcttatgttc gatttgaacg ttatacgctg
    12001 gtttattttg ataccgttga tgagatcaaa gatcgggtga attgattaaa aataagccag
    12061 atggacgtat gggcaaggat tatggtttta tttgtcatac aaataaatat aataggcgct
    12121 tccgatgtag acccgtattc ttcgcctgta ccacgggtcg gttttagtac aggcgttttc
    12181 tttaaatatc cttcaggaac gtttacgcgg catcatgcgt agaagtgtgt tgtccttcca
    12241 gaaatagtgg tgtgccagtg cagccgcagc gtgcaacccg atgacaaaat atcccagatt
    12301 cgccagcgtc tcatgccacg actttaagct atcaacccgt tcgaaattgg cctctgaagc
    12361 gtaaggcatc gtcaaaccaa acgcaaacca cgggttgccc cggttataca tcatcaccaa
    12421 accaatcact ggcagcgcaa taaacaggag ataaatcacc aaatgtccca aatgcgccag
    12481 tcccgtcatc atcggttttg gcttaggtat aatcggcggg gttgggtatt tcagtcttaa
    12541 caaaagacga accaccatca gcacgagaat tgagatacca caggaaacat gaaccatgtt
    12601 gatgagtggc cgatcgctac gtgggaagaa accacgaaac tccattgcgc aatacgctgc
    12661 gataaccagc aaaaagacca gccagtgaat gctgatttgt aaccttgagt atttattttc
    12721 cataacattt cctgctttaa cataattttc cgttaacata acgggctttt ctcaaaattt
    12781 cattaaatat tgttcacccg ttttcagtac gttacgtttc actgtaagaa tggagattgc
    12841 ccatcccaca atcctggtct aagcctggaa aggatcaatt ttcatccgaa tgttcctgac
    12901 aggagaaaac catgagtaaa gttggtatta acggttttgg tcgtatcggt cgactggtgt
    12961 tgcgtcgatt acttgaagtc aaaagcaaca tagacgttgt cgctattaat gatctcactt
    13021 ccccaaaaat tctcgcctac ctgctgaaac atgattcaaa ctacggacca ttcctctgga
    13081 gcgttgattt tacggaagat tcacttatcg ttgatgggaa aagtatcgcg gtttacgccg
    13141 aaaaagaggc taaaaatatt ccgtggaaag cgaaaggtgc agaaatcatt gtcgaatgta
    13201 ctggctttta tacctccgcc gagaaatcgc aggcgcatct tgatgctggt gcgaagaagg
    13261 tgttgatttc cgcccctgcc ggtgaaatga aaactatcgt ttataacgtc aatgacgaca
    13321 ctctggatgg caacgacacc attgtttccg tggcgtcatg caccactaac tgtcttgcgc
    13381 cgatggccaa agccttgcat gacagtttcg ggatagaagt cggcacgatg acgaccattc
    13441 atgcctatac tggcacccag tcactggtgg atggcccacg tggtaaagat ttacgtgctt
    13501 cacgcgcagc ggcagaaaat atcattcccc acactacggg ggcggcaaaa gccattggtc
    13561 tggtgatccc ggaactgagc ggcaaactga aaggtcatgc gcaacgcgtg ccggtgaaaa
    13621 caggttcggt cactgagctg gtgtccattc tcggaaaaaa agtgactgcc gaagaggtga
    13681 ataacgcact taaacaagca accaccaata acgagtcatt tggttatacc gatgaagaaa
    13741 tagtctcttc cgatatcatt ggcagccatt tcggttcggt gtttgatgcc acgcaaacgg
    13801 aaattaccgc cgtgggcgat ttacaactgg tgaaaacggt cgcctggtac gataacgaat
    13861 atggcttcgt cacgcagctt attcgcaccc tcgaaaaatt cgctaaactc tgacgcgcac
    13921 aggcggagga aaaaacctcc gcctctttta ctcattacga ctgtaaataa accacctggg
    13981 tctgcagata ttcatgcagg ccatgtttac catctgcgcc gccaataccg gatttacgcc
    14041 atccggcgtg gaagccttgc atagcttcga agttttcacg gttgatgtaa gtttcaccaa
    14101 acttcagccc tttaatggct ttcatcgcga cgttcagatt ttgggtatag attgatgagg
    14161 tcaggccgta atcactgtca ttagccattg agatagcatc ttccagcgtg tcaaatgcga
    14221 ccaccggcag aaccgggcca aaggtttcct catgcataat cgacatttcc tggcgaacat
    14281 ccagcagcaa tgtcggcgga taataatatc ctttcccgtc tactgctttg ccacccaacg
    14341 ccactctcgc cccttcttct actgcgcgcg ccactttttg ctcgaccctt tccagcgccg
    14401 cggcgttaat caacggcccc atcgcaatgt cgttgcgttc agcggggtta ccaaattgaa
    14461 ccgcctgcat cgcttcaccc agccgattga cgaactgatc ataaatgcct ttctgtacat
    14521 aaacacgttc tgcacagtta cacacttgcc cactattaat gacgcgtgaa tcaacgatgg
    14581 ctttgactgc cagttcaaga tcggcatcgt ccattacgat agctggtgct ttaccaccca
    14641 attccaggca cactttggtg atgtttttcg ccgcagtcgc cataatcttc tcacctgcag
    14701 agacgctgcc tgtcatactg accattgcga cctttgggtt acccgccagt tcttgcccaa
    14761 cggtttcacc acgccccagt acaaggttaa acacgccgcg cggaaggcct atttcatcga
    14821 cgattttggc gaatgcaatc gcattgtttg gcgtaaattc actgggttta atgacgatgg
    14881 tattaccggt caaaagagcg ggagccattt tgcgggcaat gaggaagaac gggaagttcc
    14941 acggcagaat gccggtagtc acaccaagcg cacgtttaaa caaaagaata ttttctcctg
    15001 gacgatcgct ttgaataatc tcgccctcgt aacgccgtgc ccactccgcc atgtaatcga
    15061 tatagtcggc agtaaaagcc acttcgactt cagccagctg ctggatcttg cccccttctt
    15121 caacaatcag cgcactgatt tcactggcgc gttcgcggat gccggcggag attttgcgca
    15181 accaactggc gcgttcaata gcaggcaacg cttcccattc tggttgtgca cgttctgctg
    15241 catcgattgc cttacgggca tcctcggcct gaccatcggg tatgcgggaa atgacagcct
    15301 ctgcagcagg gtttaccaca tcaatccatg cgtctccacg ccaggtaaca aactgtccat
    15361 cgatatacat aggatgttga acgggtactg acatgggcga ctcctgtgat ttatatgttt
    15421 gttttcggtg aatgcattgt taattaacat caatcatcag agagacggca tcgtgaaagg
    15481 acaattctgt gaagggcttc ataaaaacaa gtgtaaaagg aataaaaggc agaaataaaa
    15541 gtcaggagtc atgcccgttt gcaaacaagt ggttcacaaa cgggcaattt ttaacgtaac
    15601 gagcgacttt ccatcgcctc gatgagcacc gcatcatgtt tcagcgtttg ccatgcatgg
    15661 atgacttctg ccggaaaatc aacgtgaacg ataaaatctc gcccgcgggg accgtagcca
    15721 tcgctatcat cgcgtaaacg cggcagctcg ccagtgagta gtgagagata acgctcaacc
    15781 ggccataatc cttgtaactg attaataaag attacactgt ctgcgttatt tcctaactga
    15841 ggaacgaatc cgggataact taaccagcgt ggtgcatcgg gattgtcccc agtcatacgg
    15901 cggaacgtcg ccatcgtgcg ccgctgcatg gtggggtcct gaacaacgat agccgtatga
    15961 actcgttcta cggcctgatt caatagcgcg atgctaaagc gtgcgttttc accgcagttt
    16021 gttgactggt cttcaatcca gattttttca tgcggaatgt gccagaactg atgagcgata
    16081 tccgccagga tggtcgcttc tgctctgcca gtggtgcgga tagtgttgta gtgcggatgc
    16141 tgtgcgatgg cgctatacaa aaaagttgtc gagtgaccga taccaccact aatcagtaaa
    16201 ggaatttgct gatcgcgagc aatcttacat gccgcgtcga tagtcggcat aaccgcattg
    16261 cctgcaagga tcacgcaatc ggcctgatac ggcacctcac cggagaaatc atcctgcgcc
    16321 agccactgtc cgataacatt tatggcatct atagttgccg gcgaaagcgt cggaaacggg
    16381 gtaatgttca tggttcctcc ttatcgttcc cttaagaata ctgcgtgaga taatagtgag
    16441 aagcggagca ctctgaaagg aggaagtcgt atgtccttaa ccgccactcc gcaagcctga
    16501 catatctttt aaggaaatgc tgtagataaa cattattcat cttgcagcag agaattgtat
    16561 ttttctgaat tcctgaattt ttacccttac cgttttagaa ttaataaaaa acccggcgct
    16621 aaatgcttac cgggtttttt ctttatcagg caaatagcag ggttaaccgc taatctgctc
    16681 catcgcctgc aaaatacgct tatctgaaat cggataaggc gtaccaagtt gttgagcgaa
    16741 gtaactaacg cgcaactctt ctatcatcca acggatctct ttcacgtctt catcctcacg
    16801 acgtgcgggc ggcagtttgt tgatccattg ctgccacgcc tgctggacgt tttcgacttt
    16861 cagcatctga gcacggtcgc gatgtggatc aaccgccagt ttttccagcc gtttttcaat
    16921 cgcctgcaaa tatcgcagcg tgtcgcccag ccgtttgaag ccgttaccag tgacaaaacc
    16981 gcgatatacc aacccgccca tctgcgcttt aatgtcagaa agccccagtg ccatggtcat
    17041 atccacccgc cctttcagac gtttgttgat attgaacacc gcagtgagga tttgctcgac
    17101 ctgcttcgca atatccacca ccgtgtcatt cagttcggcg cgcacttttt catgcagcgc
    17161 agcaaagcct tcttccgtcc agaccgggcc accattggcg tcgatcaatt gatccacacc
    17221 gcaggagata cagtcgtcga tcagctccag cactttgcca tacgggttaa agtacagtcc
    17281 cagcttggct ttgttcggta acttttcatg taaatatttg attggcgatg gaatattcag
    17341 cagcagtaga cggcgaagac cgttccacat tgcctgcttt tgctccagcg ggttatcaaa
    17401 cagtttgatc gccacactgt cgcgctcatc caccagcgcc ggccacgcct tcactttgta
    17461 gttgccacgc ttctgttcgt agctttccgg cagctgacca aaactccaga tatgtaagcc
    17521 gctctgctcg ataccgtcat ccgccaccgc agatagcgtt tcctgcactt tgcctttcag
    17581 cgcatctttc agatcttgta gcgagcgccc ttcttttagc ttcttgtttt tgtcatccac
    17641 cacgcggaag gtaattttca ggtgatcagg cacctgatcc cagtgccagt cttcgcggtc
    17701 aacggtaacg ccggtcatcc gccgtaactc gcgctcaagg ctgtcgagca acggtaactc
    17761 cagcggtttg acgcggccta aaaacgcttc ggcatagttt ggcgcgggta caaaattacg
    17821 gcgtaccggt ttcggcaacg atttaatcag agcaatcacc agttcgcggc gcagaccggg
    17881 gatctgccat tcaaacccgc tttcctcaac ctggttaagt aacggcagcg gaatatgtac
    17941 ggtcacaccg tcagcatccg cgccgggctc aaactgatag ctcaaacgca gcttgagatt
    18001 gccctgatgc cagaagttcg ggtaatccag cttgctgatt ttttctgcgc cctctttgat
    18061 caacatgctt ttttcaaagt tgagcaaatc aggggtttcg cggctgactt ttttccacca
    18121 gctgtcgaag tgacgagcgg agattacatc gtggctgatg cgctggtcgt agaactcaaa
    18181 caacgtttcg tcatcaacca gaatatcgcg gcgacgtgat ttgtgttcca gctcttccac
    18241 ttcggcccgc agtttcaggt tttcacggaa gaatgcgtga cgcgtctgcc agtcaccttc
    18301 caccagcgca tggcgaataa agagttcacg gcataacgcc ggatcgatct ggctgtagtt
    18361 gaccttgcgc gcggcaacaa tcggcaaacc ataaacagtg actttttccg ttgccatcac
    18421 cgcgccctgc gcccgttccc agtgaggttc gctgtaggtg cgtttaatca aatgctgagc
    18481 gacaggctcc acccattccg ggtcgatacg cgcagcaatg cgcccccaca ggcggctggt
    18541 ttctaccagt tccgccacca ttacccattt cggcggtttt ttgaataaac cagaaccggg
    18601 aaagatggag aaacgcgcgt tacgtgcgcc ggtatattct tgtttatcgg catctttcat
    18661 gccgatatgg gaaagtaaac ccgtcagcaa cgcaatgtga atttcgcgat actccgccgg
    18721 ttcgctgtta accggaatgc caagttcttt caccacctgg cgcaactggg tgtagatatc
    18781 ctgccattcg cgcacgcgca gatagttgag ataatcgata cgacacaggc gacggaaggc
    18841 gttggaagaa agcgcctttt gctgctcgcc aagataattc cacagattca caaacgcgag
    18901 gaagtcagac tctttgtcgt ggaagcgacg atgtttttcg tccgatgcct gctgtttatc
    18961 catcggacgc tcgcgcggat cctgaatgga aagcgcggac gtgataatca tcgcctcacg
    19021 cacgcagcca tgtttttgcg cttccagcac catacgcgcc agacgtggat cgacaggcaa
    19081 ctgcgagagc tggcgaccga gcggcgtcag tttataggcg ctggcctgtt catccgtggt
    19141 gatcgcgccc agttcttcga gcagacgcac gccatcctgg atattgcgtt tatccggtgc
    19201 ttcgacaaac gggaacgcag cgatatcgcc cagccccagc gcggtcatct gcaaaataac
    19261 cgaggccagg ttggtacgca gaatctccgg atcggtaaac tccgggcgcg aaaggaaatc
    19321 gtcttcggaa tagagacgaa tacagatccc ttcggacaca cgcccacagc ggcctttacg
    19381 ctgattggca gacgcctgcg aaatgggttc aatcggcaaa cgctgcactt tggtgcgata
    19441 gctgtagcgg ctgatacgtg ctgtaccggg gtcgataacg tatttaatcc ccggtacggt
    19501 cagcgaggtt tccgcgacgt tggtcgccag cacaatgcgc cgtccgctgt gcgactggaa
    19561 cacgcggttc tgttcgctgt tcgaaagccg cgcataaagc ggcaagattt cggtatgacg
    19621 cagatttagc ttgttcagcg catcggcggt atcgcgaatt tcccgctcac cactcataaa
    19681 gatcagaatg tcgccagggc tttcctgact cagttcgtct acggcgtcaa aaatcgcctg
    19741 caactgatcg cgctcggtgt cgtcggcttc ttcaacaatc ggtcgatagc gcacttccac
    19801 cggataggtc cgaccggaga cttcgataat cggcgcatta ttaaagtggc gcgaaaagcg
    19861 ttccgggtcg atagtcgcgg aagtgataat gatttttagg tcaggacgcc gcggcagcaa
    19921 ctctttcaaa tagccgagca gaaaatcgat attcaggctg cgttcgtgtg cttcgtcaat
    19981 aatgatggtg tcgtactgca tcagcaggcg gtcttgctgg atctccgcca gcaggatacc
    20041 gtcggtcatc agcttgacca tcgtgttatc actaacgtga tcgctgaaac gcactttgta
    20101 accgatgcaa ccgcccggct ccgttttcag ctcttccgca atacggttcg ccaccgttct
    20161 tgccgccagg cgacgcggct gggtatggcc gatcagccct ttaatcccgc gccccagctc
    20221 catacagatt ttcggtaact gagtcgtttt accagaaccc gtttccccgg cgacgatcac
    20281 cacctggtga tcacgaatcg cttcgagaat gtcctgtttt ttctgactaa ccggtaaatt
    20341 gtcaggataa gtaatttccg gtcgtgccgc ttcacgcagc aggactttcc ctgccgcctg
    20401 gtcaatctct ttcgccatct cctggaaaat ggcctgttgt gcatcaggat ttttaacctt
    20461 cttaacaccg tgcagacgcc gagaaaaacg cagtctgtcg cgcagcatca gcgaatccag
    20521 ccgctgctgc aaggccgtaa aggtcaattt ttgttgttct gtcataacgt tagtgggcag
    20581 tagtctgcct gattaatttt cttttaaatg atagatatag agtaccacat cgcggctttc
    20641 tatgcgttgt tcaataaatt cgaacatagg cttcgattta ttgcgctatc tctgtggcag
    20701 gattgtgaat aaagtgtcag caagcaacgg ggcatcgccc attcaaacat ctataaggaa
    20761 acaccatgag caaggtatta gttcttaaat ccagcatcct ggcagggtac tctcagtcta
    20821 atcagttgtc cgattatttt gttgaacaat ggcgcgaaaa gcactccgct gatgaaatca
    20881 ccgttcgcga cctggctgca aatccgattc cggtactgga tggcgaactg gttggtgcac
    20941 tgcgtccgag cgatgcgccg ctgactccgc gtcagcagga agctctggcg ctttccgatg
    21001 agctgattgc cgagctgaaa gcccacgacg ttatcgttat tgcggtaccg atgtataact
    21061 tcaacatctc aactcagttg aaaaactatt ttgacctggt tgcacgcgca ggcgttactt
    21121 tccgctatac cgagaacggt ccggaaggtc tggtaacggg taaaaaagcc atcgttatta
    21181 ccagccgcgg tgggatccac aaagatggac caacggacct ggtgacgccg tatctgtcca
    21241 cgttcctcgg ctttatcggc attaccgatg tgaaatttgt cttcgccgaa gggatcgcat
    21301 acggtccgga aatggcagcg aaagcgcagt ctgacgcgaa agcggccatc gacagcattg
    21361 ttgctgcata accggacacg atcttgatgt ggactgtagt tcagtccaca tctcaatcca
    21421 cttacctgtt ttcccataat ttcagcatcg ctttgtgatc ttccttaagc acaatatgcg
    21481 aacgtctggc tcgaatataa ctaatcgctt catcaacggt tttacagtgt ccgtaacata
    21541 acaaccatgc cgccactacc agcgcactgc gcgataatcc caacgcgcaa tggaccagaa
    21601 cgctgccttg ctcttcgcgt aatgtttcca gcatcgccac ggcctgtcgg agctccccct
    21661 cttccggaac caccagatcc agcatcggta cacagaaata gagtcgatct tttgtcgctc
    21721 gtccccgagg gaattcaaag gtgacgtcca gaaccgcatt ctgtgccgga atatgtcgtg
    21781 gaaacgcccc taaataaaca ccagcagtaa ttttgctcac cggctccagg cgacgacaaa
    21841 accaacgcat agacagccac atcccgatgc gccagggcaa tgtcagccag taaacggcgg
    21901 gcgggagttt cccctgacta tctttccctg ttgttatcgc gccaagccca ccgtacccac
    21961 ggccaataat gagtagcgat aatactggcc aacataacca gactgaccac cataactgaa
    22021 tcatcaccat tagctccatc aacacaatgc acgagcccgc gcctacgaca tatggcagtg
    22081 ctattttgat tcgacgttga tcaggtttct gataattcca acgacgatcg acgggcacca
    22141 tccagtcaat caacatacct accgccagcc ctgtgatgac atcaataaaa tgatgctgcc
    22201 aggtcgtcag agtagaaata gcgatgagta aaaaccatcc gccacagact ttacgccacc
    22261 tctcagccag atgctgacga aagtgacgcc aaagtagcca gcagagaata atatgcagcg
    22321 aaggagactg gttataaggc agatcaaaca gttcaagttg cgaaaatagc catcccgtca
    22381 ccccactcac ttcaggacgg ataaaactaa acttcagcgg atagagcaaa aaaccgcagc
    22441 aggccattac cgttgccaga ataagccggt ggacaagtcg gcgctgttcg aatgtcgtgc
    22501 tacaaacgaa cagcgaaaat ccatataaaa gatccagact ccagtaagga acaatagtcc
    22561 agggaagaaa agggatcgcc gtttcccaac cgaatacctg actggggata tcatggctgt
    22621 taaggtcctg aaccgcggtg aactgattaa gagatccata ggtgaagaag aaaaacgggg
    22681 ccagcaacaa taaccagcca gcgccttgta gcaatacgtt ccgacgttca gttatcacga
    22741 cgcaccgcca tcgaaaccgt aaaaataccc cactcatcaa tccgttgtgt gcatttatca
    22801 aatccggcat cacgcacgag tgaatccatc tccccttgcg aacgcacgcg cattacccac
    22861 ggtttaccat ctttatgact ggttaacacc ccggcaatca tctccagttg agggtgccac
    22921 ggctgcccgg tgtagatgag aatgccgccc ggttcgatgg cattggcaag acctgcgagt
    22981 gagtttttta cctgctcatt ttcgggaaaa agctcataca ggccagagac aatcgccagc
    23041 gtaggccgcg gagttaacgc gctgagttcc tccgggttaa acgcatcgcc ctgttcaaaa
    23101 cgcacccgcc cagacattcc ccgttgggca atcatcgctt gcccctgtgc aacatttaac
    23161 tcactgtaat cacgtaacaa aatatcgctt acggcaggct cgtttgccag cgcatccagt
    23221 acatagcgcc catgccctgc ggcaatgtca accacgcgga cggctaaacc tttggcgtgg
    23281 agatcggcaa cggcttgttt aatcagtatt tgtaaatggg ttttacgctg gcgaataccg
    23341 cgccagccaa cactgttcag gtagattttg tcaattaagc gcccgaatgc gttactgcct
    23401 tgcggttgat tttgatagac ataatccagg gaactgcctg aatcaaagcc ggtgctcatt
    23461 ccgagatgca ggcccgcaga gtgcgccccg aacaatttca tcgcctttcg cataaagcga
    23521 tacgccaaat caaccggcga taatggcacg ggtccacctg aaaggagccg ccagcgatcc
    23581 gctgatggtc cagtgcggtc ttcatgctga taatcaaatt tttgtgactt gttagcatat
    23641 aaacgactaa taaagctttg cattttttca aatgcctgcg ccctgttctc ttcacccaac
    23701 gtgtcgtgat aaaagcctgg cagcagatgc agctctttca gagggctacg taatctctga
    23761 taaaaatcaa tctgcggttg gcgatgcacc acatagtcat cgcctgatat cagaagttgc
    23821 gtggggagcg taatcgccgc cgcatctcta ataatacgtt cagacgtttt gtagagatcg
    23881 agcaagatat taacggcaat cgcccgtgtg atcagcggat cattattgaa actcgccacc
    23941 cgttgccgat cgtgggtcaa atagcgtcct ttcacatagg aattaataaa aaacagacca
    24001 cgcagacgat gccataacgc cagcgcagga cgtgccagcg gtacatacaa tttaacctta
    24061 aaggccggag aagccagcac cagcccgcga attgcaggcg cataatcatg aacccatgtg
    24121 gcaaccagca ctgcgccgac gctttgcgcg atcactacca cctcatccag tccgacctgg
    24181 ctatcgctgg cagcaaaacg aacaaattca tcgacatcct gcactgaacg cgcaagagat
    24241 gggctataac cacgcgaccc cgaagtttgt ccatgccctc gggcatccca ggcataaaaa
    24301 acagtatctg gcatcgccag ttcatcaacg agatgttgta gacgaccaga atgttcatga
    24361 ccgcgatgaa ataagacgat gaccttttta gccccgggct gtaaagcggg ccagtgccga
    24421 taaaacaacg ctgtattatc actgctggta aaaaaatgtt ccccatggat gcgtgaattt
    24481 tccattttct ctattttcct tcagtaacag cagtagcgaa taaaataaaa aaataccgga
    24541 gcggtgaaaa ttaatgagtc aatcctgtca aggagtccgc catgtcctgg taatagtttc
    24601 ccactatctt taacaccaat atctcgtttg atggctgaca tcacgacatc gccgcaaaaa
    24661 ccactaatac caattaacaa acccgctaat aatgcctgta atgtatttag cggtgtcagc
    24721 aacggtccaa taatcagtga cgcaatcatg gtggtgatta cgccccccac cagaccttcc
    24781 agcgtttttc ccgggctgac tttggggacc acttttcttc tgccgcagga tttcccccat
    24841 aaatactgtg caatatcgtt tgactcggtt aatgccaaaa gaaataacac cagtaacgcc
    24901 ccgccctgga tatttgtggt tggcaacatt aataaccagg cggcatgact caaggcgaaa
    24961 acggtcatta tccagcccca aaaaatggcg ctcacggtat gcagaaaacc ggaggggtcc
    25021 cccataaaaa cttgcccggt ggctaatatt agaaacccag ccaaaggtat aaataataga
    25081 tacagctcaa agcaattaaa accgataaac aaatagttaa gaggaataac ccaatataac
    25141 caccgcggaa aatgtacaga tataagcgta caatattctt tcaatgccag aaaacttatc
    25201 agcgcaaaaa acgtcaacgt catccagcgt ggacctgaaa tcaccagtga aaaacaaatg
    25261 acgatgcccc accaggtacg aatacgtaat gtaacttcat cctcttttct ctccggtaac
    25321 cgccatagca gaaaatggtt tatcagcgtt gctaaaatta acaacgcgaa aagtgttgcc
    25381 agagattttt ccagcattat ctttcagcgc tcataagaag cacactccga caacgattaa
    25441 tcgcagtcca gagaagcaga attgaagcaa tgctccataa aagattattc cactgcatcc
    25501 actgtggata aatggcaacg gccagacccc acagaccaaa tattaacgcg cgatcgcttt
    25561 tgccaaatgg accggcataa ctgcgaacac cgttaatcgt ctgcgcgagt aaaccgcaaa
    25621 actcggtcaa tatggtgcaa aataacatga gtatcacgag tgatgcgtta ctttccggta
    25681 aaaataaaaa tggtaaatag agcgcaatat cggaaataac atcaccagtc tcattcaaaa
    25741 ttgccccaag ccgtgtttgc tgattgcact cacgcgccaa catgccatcc agcgcattga
    25801 gcgccatacg gataaaaagc acgatgggca atagcataaa gaggatgggt tgtgccacca
    25861 acatcaacag caatccggta agaagagaaa gcgccagtgc agcaagagtg atgtgattcg
    25921 ctgtaacgtg gtgcttgtaa agccaaaaca tcgtcggcct taacagcgac tgaaagagcg
    25981 gttttatctg gtagagcgtc attatcaatc cttgataatt cggtttgcgc tatggtgcat
    26041 caattcccct gacggagata tcgcaacacg ccgcttcatt aaaaattcag aatatatcaa
    26101 ggtattgggg actcactgtc aggaataatt atccccaatt aatcaaaggg ttaagaaata
    26161 ttacgttgtg gatcaataca actcatcaag ttttctcatc gcttgttccc gtattgcgcg
    26221 cgtcttaccg ttaagcgcca gctggcggac taaatccagt tcagggttaa caagctctaa
    26281 cgccagcaaa ggatcacgtt tcagcctcgc ctttaataca tcaactggaa aacggggatt
    26341 attcatcgcc acaatccacc actcgggatc aatttctgcc gccagtactg caggcggtgt
    26401 gtggcggttg ttaagtgcgc cgaacttgaa aaatgtgaga ttgcttttcg ccagtgtact
    26461 ccaccatata cgatccggtt gttttgccag tacatccagt tgtgctaacg catcggggtc
    26521 gagtgcataa tagacgcgta atgcttcatc aacctgcgca cgggagctgt tttcctgcct
    26581 cagaaaaaac ttcgcccaaa tatcattgcg tggcgtattt tgctgtaatt ccttgaggac
    26641 aacagcgggt aaattatccc aactaactaa tgcactctct aaaaagtccc agtcttccaa
    26701 acgtatcagg gcggtggcat ttaatagcat ctgatcatca acttcaccgt ggctgataag
    26761 ctcagacaaa atcatatcag cgctggtttc atacgcagct gaatctgctt tcggattatt
    26821 tttccgatgc gataaagcac gttgccaaat tttttgtttc atcgacgtgc tcagatactg
    26881 atttttcgcc agattactcc acccctcagg atcgttgaaa cgtgtcaata accgttgtag
    26941 agcagcgtca gagtgaatta cctgcatatc ccgacagcaa aagtaggcag gggatttttt
    27001 ctccatcctt ttaacaaaat taaactgtaa gttatctggc aatgctaaaa ataacacacg
    27061 tattagatca tcgtcattct cgaatttttg gtataaatag gctgcaactt tgtcactctc
    27121 ttgtggcgtc atccgcaata attgaccatg gtgtttcatc tctgccagct tgaacgctaa
    27181 gcttgtgaca acattgtttg atgaatcgtt cgccaactgc attgcctgat tgtaggtgag
    27241 ttgtcctcca gcaccgacct gacgaataga ggaatcatca ctgcggatat agccatcaat
    27301 aatggattca ggcaataacc acgcatcttt ataaagctcc tgaatgatca tatcccaatc
    27361 tcttttcgcc agagtcatta cgatatccag agggagattt tcacgtagcg caagattcaa
    27421 cggaccgaca ccatcaggta gacgactcat tcgcgtcatt atttcgacag agagcggttc
    27481 acggtcgagc aaccccagaa caacaggctg gtaacccaat tgctgatcgg tcaactccag
    27541 aatctcattg tcaggcatat atctcgccat actttcaatc gtttgttcat cattgctatt
    27601 aataagttgt ttgcgtaggt tagctgaaat tcctttaagc cttgggagag ttcgcattat
    27661 ggcaagatca aatgtcgcaa tatctcgtcc ttcaaatgca gcaacgttgc cttcatccag
    27721 catttgcaat tcctgcacta actcaggaga aggcttaggg cgaaacagcg cttctttttt
    27781 catctgatta cagacattcc ctgccatgcg aatattgctt cgcttaaaaa tttctttcag
    27841 agcatcttca tcatttatta gaccccaata atatgcatca ggatttatta tcatcagcgc
    27901 ttttttgaca ttttcagtgt catttttgtc actcgcacgg accatgaatt cactatttgt
    27961 cgaagacgca acaacaggtg caattatgtc attgataaat attccacgcg cattaatact
    28021 ccccttaatc gaagacggat tatcgtaata tgagtcgata aataccagtc cacttgcatt
    28081 tatcgaaccg gtaattaaca gtggtgaatc cgtaactata ttcgttgccg tcacgttacc
    28141 gagaataacc catggattag aaagagagaa agtggaaata ttacctttga ctgtcaaatt
    28201 accaagaaca atcaattttt gattactctc tatattacct tctaccacta aatcgccacg
    28261 atgcactttc cagtccgatg cagataaacc gaaggggaca agattcacgc tactgttgat
    28321 agcttgttca acttgttgtt tatcttcagc ggtagccgca gaaaaaaatg gttcaatatc
    28381 aactgtattt gcaacacatc gaagttccgc ctgtactgga agactcagca taaacagggg
    28441 aagtaaacct atccagccgc aaatcctgga cttaaacata tgcactccat gccattaaaa
    28501 aagaaacaaa agggaaatgc atctttccct tttgtatatt cgttattctc gcgaaatacc
    28561 atccaacgta gacaacactt cctcagaaag atgcaatttt tcagcagcca tattctcccg
    28621 taaatgcgca acagaagacg tccctgggat cagcaaaata ttcggtgaac gttgtaacag
    28681 ccacgccagc gccacctgca tgggtgttgc acccaggctc gcagcaacat ccgacagcgt
    28741 agaggattgc agcggtgtaa agcccccgag cgggaagaac ggcacgtagg caatgccatc
    28801 gtgggccaaa gcatcaatca ttgcatcatc agcacggtgc gcgatgttgt attcgttttg
    28861 cacacagaca atttcggcaa tcttgcgcgc ctctgcaacc tgcgtcggtg tgacgttgct
    28921 caggccaata tgttttacca ggccttgttg ttgcatctct gccagcacgg tcaggctggc
    28981 ctcaatcgat ccttccgctg ggccatgacc atctcccatc ataacgcgca ggttaaccac
    29041 atccagtacg tccaggccga gattacgtag attatcgtgc accgcttttt gcaactctgc
    29101 cggagaaaag gcgggcaacc aggaagcgtc ctctccacgc cgcgcaccaa ttttagtgac
    29161 aattgtcagg tcgtcagagt aaggataaag cgcttcgcgg ataatctgat tggtgacgtg
    29221 cggaccataa aagtcgctgg tatcaatgtg attgacgccc aatgccagcg cctcgcgcag
    29281 cacagttata gcgacgtggc gatctcttgg gggaccaaaa accccaggac ctgccagttg
    29341 catcgcgcca taaccaagac ggttaacgga ttttgtaccg agagtaaatg tattgctgct
    29401 catcgcaccc tgccctgtaa taaaacggat taagaaaaat cagtatagaa acgaaaagag
    29461 ataaataatg cgagggcgta aacaagatta ctggatgagt tgctctattg tcctgaaaaa
    29521 cacaaaacaa aacgcgtaaa gcgccgcatc cggcaataaa aaaaccgcaa cctgtatcag
    29581 cgttgcggtt taggtatcac atgctaaaaa ttagaatgtt cagtatctgg acgttactgt
    29641 ttcggtcgca gattctcatc aaataccaga cgtttcctgt tcggttcaac ttcccgcaat
    29701 ggctcaactt gatgcaacgt ctgcttacaa cgagtcacca gcacctgata ctcatgcgta
    29761 ccctgctttt tccatgccag ctcctgctca ctgagttcac gaatcgcttt cgccgggctg
    29821 ccgacaatca ggtaattggc aggcatttct gctttggctt tcacaaatgc ggatgcgcca
    29881 acaatgctgt tctcgccaat caccgcgccg tccatcacta ccgcgttcat tcccactaat
    29941 gcattgcggc ggataatgca accatgaagg atagcgctat gaccaatatg tccatcttct
    30001 tccacgacag tatcctgctc gggaaaaccg tgcataacgc aattatcctg aatgttcgcg
    30061 ccatctttca ccacgatacg accaaaatcg ccacgcaggc tggcatttgg cccaacgtaa
    30121 acgcccttgc ctagaataac atcgccgatc aataccgctg tcggatggac aaaactctct
    30181 tctggcacaa ccggagtcag accatctatc tggtaaattg gcatatcgcc tcctgttcaa
    30241 tattcaaacc gccaaaacgt tgaaaataca ggcttcccgg cgcaggcagt tcaccgaccg
    30301 aggtttcacc tttttcacta acgaacgcta aagcagcagg cgctacgcgc tgataaatgt
    30361 tgatacacag ctgacgcgcc gtatgccctg cccagtgcgc cggaagcaac tcctctggca
    30421 acaatgggtc tttaaggacg acacggcgat aaaaatggat cagtaaaagc tgaatatgaa
    30481 atgcacgctc cggggttaac tcgtctgctg cctcttttaa cagcggcacc agcgggcgga
    30541 atgactgaat aaaggtttcg tacatggcat tttgttcagt taaatgccag cactcttcca
    30601 ctctggcacg cagtgctgca cgagaaagcg ccagtggtat ttgcgcttca aaacaaatca
    30661 cgttatccgc cacccccgct tcatgcaaaa gtgtctgtac atccgccagt ttttgcgacg
    30721 gcgatgccat caggctgggt gccagtgcgc caaaaccttg ccagatcaac tgctttttga
    30781 catcagccag cgttgattta tctaaacctt ccgagagcaa caggagccat ttaccatccc
    30841 atgcaggttg ctctgcgcga taaattttac tttctgcccg tcgcgtcagg cgcaagcctt
    30901 tatcactgag gctatagaaa ctgcgtcgcc cgatgcggga aacatccagc cagccttctt
    30961 tattaagacg aaacaaagcg gtgcgcacga aacgctcacc aaatcccagc ccttccagca
    31021 aagcagccag actacccagc cagatttcac caccacgatg ggaaagcgaa tcaccataca
    31081 gagaggagat caaagatgtg ccactgaccg gaacagcgtt tacagcatgt tggataaaag
    31141 taacaagttt actcattcga ttcagtctgt tgttattgtt gtgatttatg aatcatagca
    31201 caggtcgtat cgacttctgg ccctggcgct ttacctcacc agggccagac gacacgtcag
    31261 gcaccaacaa tattgcgcag atcaaacacc cgacacgcct tgccttctga acgcgggata
    31321 ctgccacagt taacgatcat cacatcggta gagatcccca ccatcgactt aatccgatgg
    31381 cgcagctggt ggcacacctg gcaacgctgc tcatgcgtca atgtcaggct gctttctttc
    31441 aactccactt tcacagaaag tgaatcaaga tgcccccggc ggttcacctc cagttggtaa
    31501 tggggcgata aatgttcgaa tttgacaatc tcttcttcca gttgtgacgg gaagacattt
    31561 acaccgcgaa tgatcaacat gtcatcgctg cgtccgctga tgcgatccat gcggcgcata
    31621 gtccgcgccg ttcctggtaa cagtcgggtt aaatcacgcg tgcggtaacg aatgaccggc
    31681 aatgcttctt tggtcagcgt ggtgaataac agttcgccat gttcaccatc ggcaagcggt
    31741 gtgccgtcat gaggattaac aatttcagga tagaaatgat cttcccaaat ggttgggccg
    31801 tcggtagttt ccagacactc catcgccacc cccggcccca tcacttctga caggccataa
    31861 atatccagtg cggtgatccc cagacgccgt tcaatctctt tacgcatggc ctgtgtccac
    31921 ggctcagcac caaacactcc aacccgcagc gagcaaccgc tggcatcacc gcccaactgc
    31981 cgctccagct cttcaatcag gttaaggcaa taagatggcg taaccatgat catatctggt
    32041 tgaaaatcgc ggatcagttg cgcttgtttt tccgtctgcc caccagacat cgggatcacc
    32101 gtagcgccca gacgttcggc accatagtgc gcgcccagcc caccagtaaa caggccgtaa
    32161 ccataggcaa catgaatttt gtctttgggc gtgccacctg ccgcacgcaa ggaacgcgct
    32221 acaatattgg cccagttatc aatatcgttt tgcgtatagc cgacaactgt cggtttacct
    32281 gtggttcccg aagaagcatg aatgcgcacc acttgttcca tcggcacggc aaaggtgtcg
    32341 aagggatagt tgtcgcgcag atcctgtttg gtggtacagg gaaatttacg cagatctgaa
    32401 agttccctga aatcatcagg atgtacgcct gctgcgtcaa atttgcgccg atacatcggg
    32461 acattttcat acgcgtgttt gagcgtccat ttcagacgtt gtgtttgcaa cgcctgtaac
    32521 tcatcaacag acgcggtttc aatcgggtca agctttgtat tggttatcat tgtagggtac
    32581 tcgcaggttg atatgctcaa acacgctcca gaatcatggc gatgccctga ccgacaccga
    32641 tgcacatggt gcacaatgcg taacgaccgt tacgccgata cagctcatgg ctggcagcca
    32701 gtgccaggcg ggcaccactc attcccaacg gatggcctaa ggcgatagcg cctccgttgg
    32761 ggttaacatg tggggcatca tcaggcagcc ccaattcgcg tagtacaccc aacgcctggg
    32821 ccgcgaacgc ttcgttcagt tcaatcacgt ccatatcgtg aatactcagc cccgcgcgtt
    32881 ccagcacccg gcgagttgca ggcaccggac caagccccat caggcgcggt tccaccccgg
    32941 cggttgccat ggctacgatt cgcgcccgcg gtgtcagtcc ttgcgctgct gccatctgtt
    33001 cactggcaat aatcaacgca gcggctccgt cattcacccc ggaggcattg cctgcggtaa
    33061 tcaccccatt ggcacgaaat ggtgctttta acccacgtaa ctgttccagc gtcgtttccg
    33121 ggcgcagatg ctcatcatgt tgtatttctg ttacaacgcc tttcttgttt ttcaacacaa
    33181 ccggaacaat ctcctcagcc agaatgcctg aggattgcgc ttttgccgta cgttgctgac
    33241 tgcgtagcgc aaaactatct tgatcttctc gcgatatttt taacagttca gctacattct
    33301 ctgccgtttc cggcatgctg tcagttccaa attgttgagc catgagcggg ttcacaaatc
    33361 gccagccaat agtggtatca aacatctcag cctgacgaga aaatgcactg gttgccttgc
    33421 ccataacaaa cggtgcccga gacattgact ccacgccacc agcgatcagc aaatcgccat
    33481 cgcccgcttt aatcgcccgt gcggcgaacc ccagtgcgtc cagcccggaa ccacacaagc
    33541 ggttaatggt tgtgccggaa acactatgcg gcagccccgc cagtaacgtc gccatccggg
    33601 ctacgttacg gttgtcttct cccgcctgat tagcacagcc gaggatcaca tcatcgatac
    33661 actccgcatc gagacgcggg tttcgcacca gcagctcccg caaagggata gcagccagat
    33721 cgtcagcccg aacacctgat aatgccccac catagcggcc aattggcgta cgaattccgt
    33781 cacagataaa ggcttcacgc atcaggcttc tcctgtaatg gtgccgccga tgcggtgaga
    33841 tttaccgcga aacagcgcaa ccgttttttg ttgttggtta acaatttcga tgtcgtaaac
    33901 accggtttgc ttgccctgat gacgtacctg cgcagtagca gttaaggtgt ctccggcaaa
    33961 gcctggacgc aaaaaatcaa tcgtgcaggc agaagcgacg gctgccagcc cctggctatt
    34021 gcaggcgtag gcaaaggcag tatcagccag tgaaaatagc tgcccgccgt ggcaactttg
    34081 atgaccgtta agcatttgtg cagtgacggt catggtcact acagcaaagc cttcatccat
    34141 tgagataatg tcgatgccaa gcgctttggc gcaggcatcg ttctcataca ttgcatgggc
    34201 attttgccag gccttatgac tcataaccgc tctccagaag cgctcgttgg cgcagcaatg
    34261 aacatgggcg atagcgttct tcgccatagt gatgttgtag attttccagg agccttaata
    34321 ttcgctgcca gccaagttgc gctccccagg caagcgggcc atatggataa ttcaccccaa
    34381 gacgcatggc ggtatcgata tcctgttcag aggccacgcc tttttgcagc gcatcaaggg
    34441 cttcattgat gatcattgct accgttcgcc aaatcagcat tcctgggtaa tctgcaattt
    34501 gcagcactgt tttgccctgc tgttgcaggt aataaatggc cttgcgggtc gctgagtccg
    34561 ggttcaccgc tgccgcagca atggtcacca ccttgcccgc cattttgtcg accactacca
    34621 ccgggcgtgc cagtcgtatt gccagcgcct gtgccgtctc gccttgtgtc tcaatcaata
    34681 aaacatcgtc aatttccgtg acgccgtcac ttttcttttc tactttcatt gggctaaaac
    34741 tgtcgcttac cgcttccagg ccaacaactg cctcacgttc cgcgcgccag tcgtacacgc
    34801 ccagcccact tttcttgccc aaccgtccac caatcaccag ttcctgttgc accagcgaag
    34861 gtaaaaaacg acgctcctgc cagaaagcgt taaacaccga acaggtgaca gcaaaattga
    34921 cgtcctgacc aatcagatcg gttaattcca gcggccccat cgggaaacca gcgccatcgc
    34981 gaagtgcagc gtcaatcact tctggtgcag caacctgctc ttccagtgcc cgccaggcct
    35041 cggaataata aggacgcgcc acacggttaa cgataaatcc aggggtcgaa tgacagcgca
    35101 caggctgctt accccaactc aacgttagtt cacacaactg ctcaacaact tccgctgccg
    35161 ttgccagccc actgaccacc tccaccaact tcatcaccgg tgccgggtta aaaaaatgca
    35221 gccccgcaac acgttcagga ttttttacct ccgcagcaat cgcggttata gagattgacg
    35281 aagtgttagt ggtcaatagc gtttgtggcg ggcaaacttc cgccagctgt gcaaagagcg
    35341 cttttttgac ttccagacgt tcagacgccg cttcaatgac caggtccgca gctgccagcg
    35401 cgtgaatatc ggtcaccggg atcaggcgtt tcaatgtgcg ttcacaggtt tcagcagtca
    35461 gttttccccg cgtcacgcgt gaatttagcc gcgcgtgtat cccgtcgatt gcgcgggtca
    35521 gcgcttcagc agaaatgtca tacagtaaaa cctgatgtcc atgactggcg gcaacttcag
    35581 caatgccagc ccccatggtg ccgctcccaa tcactgccac agtttgcaca tttatcatca
    35641 tgctatttcc ccgtgaactg cggtgagcgt ttagccagga acgcactgac accttcacga
    35701 taatctgcgc tccgtccggc aagtcgctga tagtcgcgtt ccagatccag ttgcgtatcg
    35761 agcgtattgg tttcagcgct atttatcgct tgcttgataa gtccaagacc aaatgtcggt
    35821 tgtgtcgcca gatgccgtgc cagctgttgc gcggtatctg ccagcgtttc atcatcaaca
    35881 acctgccaga tcatccccca ttcgtgcgcc tgttcagcac tcagttgatt ccccagcagt
    35941 gccagcccca tggcgcgcgc tcgcccagca acgcgtggca gtaaccaggt tccaccgcaa
    36001 tcgggtatta agcctaactt actaaacgcc atgacgaatt ttgctgaacg ggcagcaata
    36061 acgatgtcgc accccagtgc cagtgttgcg cctgcccctg ccgccacgcc attgactgca
    36121 cagatcaccg gttttggcag ttttgccagg cgacgtacca gtgggttata gaaacgttca
    36181 actgacatcc ctaaatccgg tgcggggcct gtgggatcaa cgttacgatc gttaagatcc
    36241 tgaccagcac aaaatccgcg cccggcacct gtaagtaaca ggcaacggat agtgtcgtcg
    36301 cgctcgacct gtttcaggca ctctgccagt tgtgcgtgca tctcatcatt aaaactgttc
    36361 aggcgttccg ggcggttgag cgttagtgtc atcacgccct tttctacatg actgaggatg
    36421 aattccatca ttagcgtcct ttaaagtcgg gcgtgcgttt ttgtaagaaa gcggagatgc
    36481 cttcatgacg atcttctgtt gccgccagca aggtgaataa ctgtcgctcc tgggcaagtc
    36541 cggcttgcaa agccacttcc tgcgactggc gcagcgcttg ctttgccgct tgtaaggcca
    36601 acggcgagtg acgtgccatt ttcgatgcca gctgtaaggc gtattcgagg gttaaatcgc
    36661 tggggaagac gtcgctaacc agcccggcct gctgtgcttg ctgggcggtg atactttctc
    36721 cgctcagcac cattttgctg gctaacgatt taccgacact acggattaaa cgttgcgttc
    36781 cgcctgcgcc aggcatgatg ccgagagtga tttccggcaa cccaaaacgc gcgttctctc
    36841 cggcaaccac cacatcgcac aacaatgcca gttcgcaacc cgccccaagc gcgtaaccat
    36901 tgacggctgc gataagtggt ttattaaagg cctgcaatcg cgcccatagc tgcggacgtg
    36961 tatcgtttaa ggtggccgcg agatcttttt ctgccatttc gttgagatcg gccccagcgg
    37021 caaaaaagcg tgcattaccg gtaatcacac agaccgaaat gctggtatcg gtagccgcag
    37081 cttccagttc atttaccagt tgcatcagca gggcattatt tagcgcatta cgtgcggcgg
    37141 gacggttaag ggtcagcaac aatactcgtt gctgacggct gacgatcagt tcgctcatgc
    37201 catccccttc gcgtcaaagt caaccaccac atcgctggtc agcggcagtg cctggcaact
    37261 caacacataa cctgcggcca gttcatccgg ttccagactg taattggttt ccatcgccac
    37321 tttgccacgc agcactttgc atttgcaggt cgcacagacg ccgcctttgc aggcataggg
    37381 cagatcagcc ccctggcgca atgccgcatc gagaatgctt tcatcgtcgg cattaagcac
    37441 gatttcccga tcccgcccat cctgacgtac agtcactttt tgtccgtcac tttgcacgtt
    37501 aacgctacgt ttgacgcgcg tgccaggcgt attaaaccgc tccagatgaa tggttttatc
    37561 tggcattccc agtgctttta aggcggcttc cgtctcatcc atcatcgccg ccggaccaca
    37621 aataaatgcc tcatcataaa gacgaaaatt aatgagcgag gccccaagtg actgtaatct
    37681 ttcaccgtca atacgcccgt gaagcagatc gctgtcgagg gtttcctgac tgaaaatgca
    37741 caacaactgt aaacgctgag gatatttgtc tttcaggtct gccagtgcct ggcgaaacat
    37801 catgctctgg ctggtacggt taccgtagat cagggtgaac tgactttcag gctcggtttg
    37861 taaagtggtg gcgataatcg ccagcattgg cgtaatacct gatcctgctg caatagccag
    37921 atagcggccc tggcgttcgg cctggggctg atagccgaaa tgcccctgcg gcaccatgac
    37981 ctccagcgtc ataccctggc ggatgtgttc gcgggcatag cgggagaaac gtccgccttc
    38041 aatggctttc accgccacac taatttcgcc aggcaggtag ctgcggcaaa tggagtaaca
    38101 acggcgtaat tcttcaccat caagactggc ttttaaggtc aaatgttgac cggggcgaaa
    38161 gcgatacgcc tcctgcaaag gctggggcac cgcaaaggta atggtcaccg catcacgggt
    38221 ttccggctcg acttttgcca ctgttaagga atgaaacgtt gtcatggcat cctcaaatac
    38281 atttgaaata atcgaaaggt tcgcggcaac tatcgcagcg gtacaatgct ttgcaggccg
    38341 tggaaccaaa ttcactgata agtgtggtat ggacgctggc gcagcgcggg caacgtactt
    38401 ctggcggcaa atgggcatgg caactgtgtc cggcgggcgg gctaatgcca tactgtcgca
    38461 gacgctcacg ggcatcgggg gtcatccagt cggtggtcca tgccgggtcg agttgcagca
    38521 caacctgaac gggggtaaac ccgtgggttg tcattgcctc acgtatcgcg ccaatcaaat
    38581 gttccgttgc cggacaaccg gaataagtcg gcgtaaagcc gatcacccat ccttctccca
    38641 tctgtgtcac attacgcacc atgcctaaat cggtaatggt cagcaccggg atctccgggt
    38701 ccgggatctg gctgagtagc gcccatatct catgaacctg tggcggtgca atggtggcca
    38761 gacgttgcat acccatctcc tctgttacca ttgctgaccg ggcaagacac gctggagata
    38821 ctgcatttct gccagcatgg gtccaaggtg ttcggtatgc agtccttttt taccgccagt
    38881 gcgatacgcc tgctcttgcg gtacgttcaa tgtggcttcg ttgattccgg caaaaacttc
    38941 ggcttcccac gctgcgcgta aagtgcgtgg atcaaccgca ataccctctt cactcagtgc
    39001 aatatcaatc tcgtcggcat cgaacagttc ggcagtaaaa cgccacaact tgtcgatggc
    39061 ctgctgcatc ttttgccctg atacgtcagt accattgccc agtcgctcca gccagccacg
    39121 actaaaacgc aggtgataac gcgcttcttt aattgctttg gcagaaatcg ccgccagttg
    39181 cggatcacgg ctttccatca gacgggtaaa gagcgccaca tgccatgcat cgatgaaata
    39241 ctggcgtgca atggtgtcgg caaaattgcc gtttggttgt tcaaccaaca ataagttgct
    39301 gaactggcgc tcgtctcggg tgaaggccag ggtatcttca tcaccttctc ccgctaattc
    39361 agcggcatac gataagaagt tacgtgcctg acctaataaa tcgaggccaa tgtttgccag
    39421 tgcgagatcg atttccagtt ccggtgcgtg accgcaccat tcccccaacc gctgggagag
    39481 caccagatag ttatcgccca ggcgcaaggt gtaagccgtt aactgattca tttccgacct
    39541 cacatgtgct caatgccatc agggatggtg taaaacgttg gatggcgata gaccttgctt
    39601 tcagccggat caaaaaattc accgcgttct tccggttgcg aggcaacaat ttcactcgcc
    39661 ttcaccaccc aaattgaaca tccttcgcta cgacgggtgt aagcatcacg ggcattttcc
    39721 agtgccatcc gctcatcggc agcatgtaaa ctgccaacat ggcggtgtga taagccctgt
    39781 ttgccacgca cgaaaacttc gtataacggc cagtaaacat tactcatttt gaatctcctt
    39841 atgcgacctt acgggcaagt tgtttttgtg catgggccag cgctgcttcc cgtacccagg
    39901 taccttcttc ccaggcttta cgtttggcgt cgagcctctc ctgattacaa attccgcgac
    39961 cgttaattac ttcgttaaac tcctgccagt cgatctcacc aaagcggtag tgaccgcttt
    40021 cagtgtcaaa atgcagatcc gggtcaggaa cggtcatacc gagcatttca acctgtggaa
    40081 cggtgttgtc cacgaaacgc tggcggagtt cgtcgttggt gaaacgtttg attttccagg
    40141 cgagacttct ggcgctgttt ggcgagttat catcgtttgg cccgaacatc attaaggctg
    40201 gccaccagaa acggttaatg gcgtcttgca acatctgctt ttgagcttcg ctgccctgcg
    40261 ccagtgccat gcaggcttca aacccctgac gctggtgaaa actctcttct ttgcagattt
    40321 tcaccattgc tctggcatac gggccataag aagttcggca taacgccacc tgattgacga
    40381 tcgctgcgcc atccaccagc cagccgataa cgccgatatc agcccaactc aatgtcggat
    40441 aattaaagat ggaggaatat ttcatccgtc cgtcgagcat tttttggtag atgtcttctc
    40501 tggcacagcc cagtgtttcg gcggcgctat agagatacag accatgaccg gcttcatcct
    40561 gcactttggc taacagaatg gctttacgcc gcaaggttgg tgcgcgagtg atccagttac
    40621 cttcaggcaa cataccaaca atctcggagt gcgcatgctg cccaatctgg cggatcaatg
    40681 tctttcggta agcatcgggc atccagtcct gcggctcgat agccgtttcc tgggctatcc
    40741 gttgctcaaa gcgttcttct tgggtcacgt catcaccttt acgattccag ttttcgtgaa
    40801 ttaattaaaa acacgaatca cgttgtttta tgaaagttac acaatggtta actaaagtca
    40861 caaattttgt atacttgaac tgtgacagat ttcgcactct attttgataa ctcattattt
    40921 atcagtgcat taatgttttt agtagttgat atacagatca cgcagttaac atttcgttaa
    40981 aagatccctt gctttttatg attcgcgatt taactattag caacagaaat gtgaaacatc
    41041 tggagagtag cgatgcagca gttagccagt ttcttatccg gtacctggca gtctggccgg
    41101 ggccgtagcc gtttgattca ccacgctatt agcggcgagg cgttatggga agtgaccagt
    41161 gaaggtcttg atatggcggc tgcccgccag tttgccattg aaaaaggtgc ccccgccctc
    41221 cgcgcgatga cctttatcga acgtgcggcg atgcttaaag cggtcgctaa acatctgctg
    41281 agtgaaaaag agcgtttcta tgctctttct gcgcaaacag gcgcaacgcg ggcagacagt
    41341 tgggttgata ttgaaggtgg cattgggacg ttatttactt acgccagcct cggtagccgg
    41401 gagctgcctg acgatacgct gtggccggaa gatgaattga tccccttatc gaaagaaggt
    41461 ggatttgccg cgcgccatgt actgacctcg aagtcaggcg tggcagtgca tattaacgcc
    41521 tttaacttcc cctgctgggg aatgctggaa aagctggcac caacgtggct gggcggaatg
    41581 ccagccatca tcaaaccagc taccgcgacg gcccaactga ctcaggcgat ggtgaaatca
    41641 attgtcgata gtggtcttgt tcccgaaggc gcaattagtc tgatctgcgg tagtgctggc
    41701 gacttgttgg atcatctgga cagccaggat gtggtgactt tcacggggtc agcggcgacc
    41761 ggacagatgc tgcgagttca gccaaatatc gtcgccaaat ctatcccctt cactatggaa
    41821 gctgattccc tgaactgctg cgtactgggc gaagatgtca ccccggatca accggagttt
    41881 gcgctgttta ttcgtgaagt tgtgcgtgag atgaccacaa aagccgggca aaaatgtacg
    41941 gcaatccggc ggattattgt gccgcaggca ttggttaatg ctgtcagtga tgctctggtt
    42001 gcgcgattac agaaagtcgt ggtcggtgat cctgctcagg aaggcgtgaa aatgggcgca
    42061 ctggtaaatg ctgagcagcg tgccgatgtg caggaaaaag tgaacatatt gctggctgca
    42121 ggatgcgaga ttcgcctcgg tggtcaggcg gatttatctg ctgcgggtgc cttcttcccg
    42181 ccaaccttat tgtactgtcc gcagccggat gaaacaccgg cggtacatgc aacagaagcc
    42241 tttggccctg tcgcaacgct aatgccagca caaaaccagc aacatgctct gcaactggct
    42301 tgtgcaggcg gcggtagcct tgcgggaacg ctggtgacgg ctgatccgca aattgcgcgt
    42361 cagtttattg ccgacgcggc acgtacgcat gggcgaattc agatcctcaa tgaagagtcg
    42421 gcaaaagaat ccaccgggca tggctcccca ctgccacaac tggtacatgg tgggcctggt
    42481 cgcgcaggag gcggtgaaga attaggtggt ttacgagcgg tgaaacatta catgcagcga
    42541 accgctgttc agggtagtcc gacgatgctt gcagctatca gtaaacagtg ggtgcgcggt
    42601 gcgaaagtcg aagaagatcg tattcatccg ttccgcaaat attttgagga gctacaacca
    42661 ggcgacagcc tgttgactcc ccgccgcaca atgacagagg ccgatattgt taactttgct
    42721 tgcctcagcg gcgatcattt ctatgcacat atggataaga ttgctgctgc cgaatctatt
    42781 ttcggtgagc gggtggtgca tgggtatttt gtgctttctg cggctgcggg tctgtttgtc
    42841 gatgccggtg ttggtccggt cattgctaac tacgggctgg aaagcttgcg ttttatcgaa
    42901 cccgtaaagc caggcgatac catccaggtg cgtctcacct gtaagcgcaa gacgctgaaa
    42961 aaacagcgta gcgcagaaga aaaaccaaca ggtgtggtgg aatgggcggt agaggtattc
    43021 aatcagcatc aaaccccggt ggcgctgtat tcaattctga cgctggtggc caggcagcac
    43081 ggtgattttg tcgattaatc ggtgaatgaa gggcaacggc caatagttgc ccttttattt
    43141 cactaagttt tgtgacgttg tcacattatg catgatgtgt acatctattt tcagggcacc
    43201 cactgtatga aaagctggca cacctgccaa acaacctggc aggtgcaggc aatccccttt
    43261 gcatcagtac tgataatgtg aacctgacta aaccgcccac agagcgcggt tgctaacaag
    43321 aacacaacat ctgacgaggt taataatggg aagcccctct ctgtattctg cccgtaaaac
    43381 aaccctggcg ttggcagtcg ccttaagttt cgcctggcaa gcgccggtat ttgcccacgg
    43441 tggtgaagcg catatggtgc caatggatga aacgcttaaa gaatttggtg ccgatgtgca
    43501 gtgggacgac tacgcccaga tctttaccct gattaaagat ggcgcgtacg tgaaagtgaa
    43561 gcctggtgcg caaacagcaa ttgttaatgg tcaacctctg gcactgcaag tgccggtagt
    43621 gatgaaagac aataaggcct gggtttctga cacctttatt aacgatgttt tccagtccgg
    43681 tctggatcaa acctttcagg tagaaaagcg ccctcaccca cttaatgcgc taactgcgga
    43741 cgaaattaaa caggccgttg aaattgttaa agcttccgcg gacttcaaac ccaatacccg
    43801 ttttactgag atctccctgc taccgccaga taaagaagct gtctgggcgt ttgcgctgga
    43861 aaacaaaccg gttgaccagc cacgcaaagc cgacgtcatt atgctcgacg gcaaacatat
    43921 catcgaagcg gtggtggatc tgcaaaacaa caaactgctc tcctggcaac ccattaaaga
    43981 cgcccacggt atggtgttgc tggatgattt cgccagtgtg cagaacatta ttaacaacag
    44041 tgaagaattt gccgctgccg tgaagaaacg cggtattact gatgcgaaaa aagtgattac
    44101 cacgccgctg accgtaggtt atttcgatgg taaagatggc ctgaaacaag atgcccggtt
    44161 gctcaaagtc atcagctatc ttgatgtcgg tgatggcaac tactgggcac atcccatcga
    44221 aaacctggtg gcggtcgttg atttagaaca gaaaaaaatc gttaagattg aagaaggtcc
    44281 ggtagttccg gtgccaatga ccgcacgccc atttgatggc cgtgaccgcg ttgctccggc
    44341 agttaagcct atgcaaatca ttgagcctga aggtaaaaat tacaccatta ctggcgatat
    44401 gattcactgg cggaactggg attttcacct cagcatgaac tctcgcgtcg ggccgatgat
    44461 ctccaccgtg acttataacg acaatggcac caaacgcaaa gtcatgtacg aaggttctct
    44521 cggcggcatg attgtgcctt acggtgatcc tgatattggc tggtacttta aagcgtatct
    44581 ggactctggt gactacggta tgggcacgct aacctcacca attgctcgtg gtaaagatgc
    44641 cccgtctaac gcagtgctcc ttaatgaaac catcgccgac tacactggcg tgccgatgga
    44701 gatccctcgc gctatcgcgg tatttgaacg ttatgccggg cctgagtata agcatcagga
    44761 aatgggccag cccaacgtca gtaccgaacg ccgggagtta gtggtgcgct ggatcagtac
    44821 agtgggtaac tatgactaca tttttgactg gatcttccat gaaaacggca ctattggcat
    44881 tgatgccggt gctacgggca tcgaagcggt gaaaggtgtt aaagcgaaaa ccatgcacga
    44941 tgagacggcg aaagatgaca cgcgctacgg cacgcttatc gatcacaata tcgtgggtac
    45001 tacacaccaa catatttata atttccgcct cgatctggat gtagatggcg agaataacag
    45061 cctggtggcg atggacccag tggtaaaacc gaatactgcc ggtggcccac gcaccagtac
    45121 catgcaagtt aatcagtaca acatcggcaa tgaacaggat gccgcgcaga agtttgatcc
    45181 gggcacgatt cgtctgttga gtaacccgaa caaagagaac cgcatgggca atccagtttc
    45241 ctatcaaatt attccttatg caggtggtac tcacccggta gcaaaaggtg cccagttcgc
    45301 gccggacgag tggatctatc atcgtttaag ctttatggac aagcagcttt gggtaacgcg
    45361 ttatcatcct ggcgagcgtt tcccggaagg caaatatccg aaccgttcta ctcatgacac
    45421 cggtcttgga caatacagta aagataacga gtcgctggac aacaccgacg ccgttgtctg
    45481 gatgaccacc ggcaccacac atgtggcccg cgccgaagag tggccgatta tgccgaccga
    45541 atgggtacat actctgctta aaccatggaa cttctttgac gaaacgccaa cgctaggggc
    45601 gctgaagaaa gataagtgat tgtttcagac aaaaaaacgg caccaggtgc cgttttttac
    45661 ttatgagcga accagattaa taccgtacac acaccgactt agtttcacac caaccgtcca
    45721 gccagtcggg gccaaaatca cggcccgttc ctgactgctt catcccacca aacggtaagt
    45781 tagcgtcaat taaggtatgg ctgtttaccc acaccgtccc tgcctgtaag cgatcgctat
    45841 attccagagc ctgggagaga ttttgcgtcc agacactggc agttaagcca tattccgtgt
    45901 cgtttgccag ttgtaacgcc tcttctccat ccgctactcg caccaggttt accaccggac
    45961 caaacacctc ttcacgagtt aagcgcaatt tagcatcggg atttaccacc agcgttggcg
    46021 caacataata cccctctccg gctggtccat tcgacccgcg aatcagctct gcttgctgtg
    46081 cctgcgcatc gtcgaggaat gaacacactt tgtcgcagtg cgcacgagaa accaaagggt
    46141 taatctgtgc aacaggtgac atccccggtc ccacttgcaa cgattttacc gcctgctcaa
    46201 atccactaac cagcgtgtca aacaacggcg cttcaatata aattcgcgaa ctggcggcgc
    46261 atacttgccc ttgattcagg aagcttccgg tcatcaagcc ttcaataacc cattgcggat
    46321 cagcatcttt taatacaatt gccgggtttt taccgcccag ttccagcgtt acacgcgtta
    46381 agtgatcagc agcagttctg gcaatacctt ttcccgtcgc ggttgaaccg gtaaaactga
    46441 ttttcgcaac atgaggatgt gacgtcaggg ccgcgccgca tacagcacct gacccggtga
    46501 cgacattaaa aacgccatca gggataccag cctcgctggc cagttccgcc acgcgcaaca
    46561 tcgtcagtgg cgtggtttcc gaaggcttaa tcacgattga acagcctgct gccagtgctg
    46621 gcatcacctt ccacatacca atcatcaacg gaaagttcca tggcacaatt cccgccacta
    46681 cgccaaccgg ctctttacgc gtccaggcct gataacgcgc cccctggggt aagggaatcg
    46741 acaagtccag cgttttaccc gcgattttgg tcgttaaccc ggcggtataa cgcatccagt
    46801 tcagcgtaca gcccacttca aaagcacgag aaatggcaat tgacttgcct tgctccaggg
    46861 tttccagttg cgccagctcc tcactgtgct gctccaccag atcagcaaaa cgtagcagaa
    46921 tacgttcacg ctctgcgggt aatcgcccgg cccagcgacg cgagacaaag gcccgccagg
    46981 cagacatgac tgcgttatct acatccgctt cgttggcatc agcagtagac gcaatttctt
    47041 gcccggtggc cggatcaaag atcgccaatc gtttttcact ttgtgcgggg cctggacgac
    47101 catcaatata aagaccgtgt tgacgatcga gaaactgttg gacctggctt aatactgcta
    47161 catgcggctc tgtcatcact tttccttatt atttacccag tgtgatttca gtgtacgcag
    47221 cgacaagaaa agtcactttt ttcgctgtga cattcgcttt gtcagctgtg acaagctccg
    47281 caaatcgtga caataaccgc ttatgtctct atgcctgaga agacttattt cctatagtca
    47341 ggctcattag cttcgtttac tgcaacacaa atgcaacaat aaaaatacat ttcacagagc
    47401 gaaaacgtgc catgaacccc gcagtggata atgagtttca gcaatggctt tcccaaatca
    47461 atcaggtatg cggaaatttt accggacacc tgctgactga gcgttacacg ggtgtactgg
    47521 atacgcattt tgccaaagga ctaaagctga gtaccgtgac aaccagcggg gtgaatttat
    47581 cccgcacctg gcaggaagta aaaggcagcg acgatgcctg gttttacacc gtttttcagc
    47641 ttagtggtca ggcaataatg gagcaggatg agcgtcaggt gcagattggc gctggcgata
    47701 ttacgttact cgatgcctca cgcccctgtt cgctttactg gcaggagtct tctaaacaga
    47761 tttcattact tttgccacgc actctgctgg aacaatattt tccccatcaa aaacctgtct
    47821 gcgcagaaag actggacgct gacttaccca tggtgcaact cagtcatcgc ctgttacagg
    47881 agagcatgaa taatccggca ctttctgaaa cagaaagtga agctgcgcta caggcgatgg
    47941 tgtgtctgct gcgcccggta cttcatcagc gggaatctgt tcaacctcgt cgtgaacgtc
    48001 agtttcaaaa agtggttacg ttgatagacg ataatattcg cgaagagata ttacgcccgg
    48061 agtggatagc cggagagaca ggtatgtcag tacgtagttt gtaccgaatg tttgccgata
    48121 aaggtttggt agtcgcgcaa tatattcgta accgtcgtct cgatttttgt gcagatgcga
    48181 ttcgccatgc cgcagatgat gaaaaactgg caggcatcgg ctttcattgg ggattttctg
    48241 accagagtca tttttcaacg gtatttaagc aacgctttgg gatgacgcca ggcgagtatc
    48301 gacgtaaatt ccgctaacta attggcgttg tgtgtactgc aacgccaatt agttaaaaca
    48361 gatatttgac tatgcatttt tcagggcgaa aggcaatttg acaggagttt ttgaatattc
    48421 accttgtttc aggaaaatag atcaatcgct ttctcctgta gcgttatccc gatattgcgg
    48481 ttttgctatt aaaattttcg cacccattta ccgtttatcc cttgtacata ttcccccggt
    48541 ttggcccgag ctacgagctt ttgcccggcg agtttcgcga tatcatctac agacacatta
    48601 ttttgctttg ccagttgttg gtaactcgct ttacgttcgg cattaatgtc ggttactaat
    48661 ttctctgttt cagcatccgt tttcagcgca accagataac cataaaatgt ttcacctacc
    48721 cgcccctgag ttctggcttc atccagagtc aatgccatta cattgctgct taccagccca
    48781 acaagaaacg cacaaaaaag taatgttttc ttcatcatcg cctcagaaaa gatcgctacg
    48841 agtttcaagc agctcttcga catctttgtc tgccttgatg atgatctcat gctcaatttt
    48901 aacgttcata ttgatagtga tcggttcctt gggcgcagcg acttcaatac gcggcgtaca
    48961 ggcagccagc ataaaagatg acgtcaacgc agccagtaaa attttcattg tttttcctca
    49021 cactcttttc cttgcggaca gtcatttccc ggcagacgtg cgttctgctc caaccatgcc
    49081 tggagattat cgccaaagcg taaactgcgc cacagcgtaa aaatattctc ttcatgatgg
    49141 taattaaggt ttaccgtacc gcttttacca tcaacgcgac tggtacctgt aatgttggcc
    49201 tgcatggtta ataaaccgag attatctaag ttaatttttg tcgatgaccg gctaatttcc
    49261 atatagcgca accagttaat tgctgaaccc gcagtcatat tgtctttcac caccgcatct
    49321 gcggtgtctt tatccagccg caaggtcatt ggcccgctat ttgccagcca accatctttc
    49381 actatccatt tttcgctgtt tagccataac ggcaatgcac cactaaatgc cccggacatg
    49441 gcgaattgtt tcggattgac ggcgctaacc agttcgctgg atgagagatt attcagccgc
    49501 aacagcgccg ggtcatgttg cggcatacgt aattgtttca tcagtacgtt accgcctaac
    49561 acatcgacgc taacatcagt caacagcaag ggttcttctt cagtccacgg ataacgccct
    49621 tgcaaatcag ccgtaatatt tttcgctgtc accagattaa tcacttcggc aattcgcaac
    49681 gtaacggggc cgcgagtccc cagatgccag gctccatcgg caaaacggaa aggcaggaca
    49741 aaatcgacac cgttaacctg attatctggc atccaggcac taccgccttt caacacgccg
    49801 tgtcctcccg cgcggaatcc ttgttcaggc gcagcagaaa atgcaacctg agcatacagt
    49861 tcaccatcgc gtaagttcat cttccagtcg ggtggcacca gcggctggaa tacagtcagt
    49921 gattgttttg gccaccaggc gttaccgcgc agacgaatac cgtcccagcg accatttacc
    49981 cgaaccgggc caatctcacc agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg
    50041 cgcccatcga cgctaaattt taaggttgat ggcggtaaca cactgccgcc agtgaacagc
    50101 gtttgcccgg cgtccagtga cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc
    50161 cagacgacgg gcttgtcgag aattaatcgc ggcttttcta ccgtcatcgt accgtattgt
    50221 aactggtcga aaccggtgga gaggtcggtc agcgtaatcg tgctgtcatg ccattcacct
    50281 tttcctgcga catcccaggt ggcattcatc ggtgtaaaac tgccttttcc ccagtagcgc
    50341 cactgccagc ggcccgcgtc agggagaaaa tcattcgcca gcccatccat atgcagcacg
    50401 aaatcgccca gttcattttc atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca
    50461 cgttgggtga cttttacacc cgctaaaggc cagcggattt catcgatgtc cagcgaatcg
    50521 atgactcttc ccttcgaacg taataacgcg cctggctcaa aggccagcgt tgggtcagtc
    50581 agacttccac ttagctgcgc gggtaagcgg gcatataaaa tgaggtccgc ctgtttcgct
    50641 tcaccggtca gttgcagagg cagctggctg ttatccatgc ttaattttcc tgggccaaaa
    50701 tttagcaccg cgttgccttt acccgcttgc ccctgggtca gcacactcag tcgtccgctg
    50761 atcagagcgt tctcaagccc tgcctgccag ttgtcgactt tgacacccag tcggccactc
    50821 aaaggaaaac ctgcatacgg ccaactccag cgaccatcgc ttacggtcaa ttgttgacga
    50881 gtaatttgcc acggcaaatc gagcaacgga tcgccgttat cccgtgccag cacaatcaat
    50941 tgcccgctat tttcctgcca gtccagctct gcatccacca gtgacggttc ctgcggcaag
    51001 tttaacgtcg cagtagcatg cccacttaca ggaagtccat ccggcacgag cggcatagta
    51061 aattccccca ccagtttaac cggcggctga ttttcaaacg cgacgacatc cagttcgctg
    51121 actgtaagtt gttgcccttt cagctggcct tgaaatttaa ctttttcgcc ctgataacgc
    51181 agttgctgga tatcagaggt taatgcgaga gagagttttc cctgccattc ctgccaggga
    51241 gaaaaaatca gtttatcgat attgatccag gtgttaggca gcatggactg ccactgcgcg
    51301 agggtttttg gagcagctgg cgattgctcc gtctgcggca atttcgccag acaagcagaa
    51361 tcaagttcta ccgtgccgac gttcaataac cagcggctgg gatgtgaaag gctggcgttg
    51421 gtgatatgcg caagctgaca atctcccacc agataacgga gatcgggaat gattaaacct
    51481 ttacgcgtaa tgcgtgggct ttcatctaat gcaatacgtg tcccgagcgg tagccagatg
    51541 cccgccagag tgggaaccca cagcccgagc gtcatcaaca gcgtcaacgg cacaagaata
    51601 atcagtaata acagcgcgag aacggcttta tatttaccca gcatgggtag ttaatatcct
    51661 gatttagcga aaaattaagc attcaatacg ggtattgtgg catgtttaac cgttcagttg
    51721 aaggttgcgc ctacactaag catagttgtt gatgaatttt tcaatatcgc catagctttc
    51781 aattaaattt gaaattttgt aaaatatttt tagtagctta aatgtgattc aacatcactg
    51841 gagaaagtct tatgaaactc gccgtttata gcacaaaaca gtacgacaag aagtacctgc
    51901 aacaggtgaa cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa
    51961 aaaccgctaa aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca
    52021 gccgcccggt gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg
    52081 ccggtttcaa taacgtcgac cttgacgcgg caaaagaact ggggctgaaa gtagtccgtg
    52141 ttccagccta tgatccagag gccgttgctg aacacgccat cggtatgatg atgacgctga
    52201 accgccgtat tcaccgcgcg tatcagcgta cccgtgacgc taacttctct ctggaaggtc
    52261 tgaccggatt tactatgtat ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg
    52321 tggcgatgct gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc
    52381 caagtgcagc ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg
    52441 aatcagacgt tatctctctg cactgcccgc tgacaccgga aaactaccat ctgttgaacg
    52501 aagccgcctt cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat
    52561 tgattgattc tcaggcagca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta
    52621 tggacgtgta tgagaacgaa cgcgatctgt tctttgaaga caaatccaac gacgtgatcc
    52681 aggatgacgt gttccgtcgt ctgtctgcct gccacaacgt gctgtttacc gggcaccagg
    52741 cattcctgac agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca
    52801 atctggaaaa aggcgaaacc tgcccgaacg aactggttta atcttgccgc tcccctgcaa
    52861 tccaggggag ctgattcaga taatccccaa tgacctttca ttctctattc ttaaaatagt
    52921 cctgagtcag aaactgtaat tgagaaccac aatgaagaaa ctagccgcgt ttgttgcgct
    52981 aagcctgctg atggcgggat gtgtaagtaa tgacaaaatt gctgttacgc cagaacagct
    53041 acagcatcat cgctttgtgc tggaaagcgt aaacggtaag cccgtgacca gcgataaaaa
    53101 tccgccagaa atcagctttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt
    53161 tagcggtgaa ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg
    53221 tatgatgtgc gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa
    53281 agaaggtgca caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac
    53341 attaacttat aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag
    53401 tgactgttcg ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc
    53461 cagttgacgg gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact
    53521 ggacatcgac gcttttaaac ctggcgctac gtgcgccgca gtcgcctgct gaacaggttc
    53581 actactacac gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctc
    53641 ttcttcctct gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc
    53701 ggcgtcgaga gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt
    53761 gccctctgtt tgatcaaaac attcattacg ctgatgtggg ggacacaaaa gcgaaaatgc
    53821 agaagaaagc catttgctaa aattgaagga ttactactgg gcgcgcagca atttcgtgcg
    53881 cccctcattt gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt
    53941 gcttcggtgg cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc
    54001 acgatggcag aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac
    54061 acaccacgcg tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct
    54121 ttgcagacgg gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc
    54181 ccgacgctgt acaaactggc aggcgaactc acaccgtttg tcctgcatgt agcggcacgt
    54241 accgttgcca cacatgcact ctctattttt ggcgatcatt ccgacgtcat ggcggtgcgc
    54301 cagacgggtt gcgcgatgtt gtgtgcggca aacgtccagg aagcgcaaga ctttgctctc
    54361 atttcgcaca tcgcgacgct gaaaagccgc gtgccattta ttcatttctt tgatggtttc
    54421 cgcacgtccc acgaaatcaa caaaattgtc ccgctggccg atgacacgat tcttgatctc
    54481 atgccgcagg ccgaaattga tgctcatcgc gcccgggcac tcaacccgga acatccggtg
    54541 atccgcggta cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaaccca
    54601 tggtacgacg cggtctatga ccatgttgaa caggcgatga atgatttctc tgccgcgaca
    54661 ggtcgtcagt atcagccgtt tgaatattac gggcatccgc aagcggaacg ggtgattatc
    54721 ctgatgggct ctgccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc
    54781 gaaaaagtcg gcgtgctgaa agttcgcctg taccgcccct tctcagccaa acacttgcta
    54841 caaactctac cgggatccgt acgtagcgtg gcggtactgg acagaaccaa agaacccggt
    54901 gcccaggcag aaccgctcta tctggatgtg atgaccgcac tggcagaagc ctttaataat
    54961 ggcaagcgcg aaactctccc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa
    55021 tttggcccgg actgtgtact ggcggtattt gccgagctca acgcggctaa accgaaagcg
    55081 cgctttacgg ttggtattta cgatgatgta accaatctgt ccctgccgtt gccggaaaac
    55141 acccagccaa actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc
    55201 gtttccgcga ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag
    55261 ggctattttg tttacgactc caaaaaggcg ggcggcctga cggtttctca ccttcgagtg
    55321 agcgaacagc cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac
    55381 cagttgcagt ttatcgataa atatcagatg gctgagcgtt taaaacctgg cggcattttc
    55441 ctgctcaaca caccgtacag cgcagatgaa gtgtggtcgc gcttgccgca agaagttcag
    55501 gccgtgttaa accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc
    55561 gaatgtggcc tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg
    55621 caaattctgc ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac
    55681 agtagcaaag gccaggatct ggtggaacgc aattggcagg ctctggcgct ggcgcgtgaa
    55741 tccgtagaag aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg
    55801 gtttccgatg ccgctcctga tttcgtgaaa accgtaaccg ctgcgatgct cgccgggctt
    55861 ggtgacgccc tccccgtttc ggcgctgccg ccagacggct cctggccgat gggcactacg
    55921 cgctgggaaa aacgtaatat tgccgaagag atccccacct ggaaagagga actctgtacc
    55981 caatgtaacc actgcgttgc cgcttgccca cactcagcta ttcgcgcaaa agtggtgccg
    56041 cctgaagcga tggaaaacgc ccctgccagc ctgcattcgc tggatgtgaa atcgcgtgat
    56101 atgcgcgggc agaaatatgt cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg
    56161 tgcgtcgaag tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg
    56221 atgtctcgcc tggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg
    56281 ccagaaatcg accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca
    56341 ccgctgtttg aatattcagg tgcttgctcc ggttgtggcg agacgccgta tattaaattg
    56401 ctgactcagc tctatggcga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt
    56461 tatggcggta acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca
    56521 tgggcgaact ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc
    56581 gatcaacacc gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg
    56641 gaattactga cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca
    56701 gctttacgcc agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat
    56761 gcactggtgg aaaaatcaat ctggctgatt ggtggcgacg gctgggctta cgatatcggc
    56821 tttggcggtc tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat
    56881 acgcaatgct attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta
    56941 actaagttcg gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg
    57001 atgtacggtc atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg
    57061 aaagcgattc aggaagcgga agcgtatccg gggccatcgc tgatcattgc ttatagcccg
    57121 tgtgaagagc atggttacga tctggcactc agccatgacc agatgcgcca actcacagct
    57181 accggcttct ggccgctata tcgcttcgat cctcgtcgtg ccgatgaagg caaactgccg
    57241 ctggcgctgg attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcaa
    57301 cgtttccgtc gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct
    57361 gcagctgatt tgcaaaaacg ctatgacttc ctggcacaaa tggccggaaa agcggaaaaa
    57421 agcaacaccg attaaatgct ctggataagg attatccaat tctaaaaaaa aagcccggac
    57481 gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca
    57541 gaaaataaaa tctgtgacaa taaaggcata taacccgc
//
LOCUS       DAZFKM010000015        57504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFKM010000015 DAZFKM010000000
VERSION     DAZFKM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(181..444)
                     /gene="cedA"
                     /locus_tag="ACHKAY_001006"
     CDS             complement(181..444)
                     /gene="cedA"
                     /locus_tag="ACHKAY_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO5699721.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            627..2888
                     /gene="katE"
                     /locus_tag="ACHKAY_001007"
     CDS             627..2888
                     /gene="katE"
                     /locus_tag="ACHKAY_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO5699722.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3147..3905)
                     /gene="chbG"
                     /locus_tag="ACHKAY_001008"
     CDS             complement(3147..3905)
                     /gene="chbG"
                     /locus_tag="ACHKAY_001008"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO5699723.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3918..5270)
                     /gene="celF"
                     /locus_tag="ACHKAY_001009"
                     /gene_synonym="chbF"
     CDS             complement(3918..5270)
                     /gene="celF"
                     /locus_tag="ACHKAY_001009"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5699724.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5375..6217)
                     /gene="chbR"
                     /locus_tag="ACHKAY_001010"
     CDS             complement(5375..6217)
                     /gene="chbR"
                     /locus_tag="ACHKAY_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO5699725.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6225..6575)
                     /gene="chbA"
                     /locus_tag="ACHKAY_001011"
     CDS             complement(6225..6575)
                     /gene="chbA"
                     /locus_tag="ACHKAY_001011"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO5699726.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6626..7984)
                     /gene="chbC"
                     /locus_tag="ACHKAY_001012"
     CDS             complement(6626..7984)
                     /gene="chbC"
                     /locus_tag="ACHKAY_001012"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO5699727.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8069..8389)
                     /gene="chbB"
                     /locus_tag="ACHKAY_001013"
     CDS             complement(8069..8389)
                     /gene="chbB"
                     /locus_tag="ACHKAY_001013"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO5699728.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8688..9026)
                     /gene="osmE"
                     /locus_tag="ACHKAY_001014"
     CDS             complement(8688..9026)
                     /gene="osmE"
                     /locus_tag="ACHKAY_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO5699729.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9228..10055
                     /gene="nadE"
                     /locus_tag="ACHKAY_001015"
     CDS             9228..10055
                     /gene="nadE"
                     /locus_tag="ACHKAY_001015"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO5699730.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10285..11172
                     /gene="cho"
                     /locus_tag="ACHKAY_001016"
     CDS             10285..11172
                     /gene="cho"
                     /locus_tag="ACHKAY_001016"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO5699731.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11132..11707)
                     /gene="ves"
                     /locus_tag="ACHKAY_001017"
     CDS             complement(11132..11707)
                     /gene="ves"
                     /locus_tag="ACHKAY_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO5699732.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11915..12400)
                     /gene="spy"
                     /locus_tag="ACHKAY_001018"
     CDS             complement(11915..12400)
                     /gene="spy"
                     /locus_tag="ACHKAY_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO5699733.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12730..13698)
                     /gene="astE"
                     /locus_tag="ACHKAY_001019"
     CDS             complement(12730..13698)
                     /gene="astE"
                     /locus_tag="ACHKAY_001019"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO5699734.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13691..15034)
                     /gene="astB"
                     /locus_tag="ACHKAY_001020"
     CDS             complement(13691..15034)
                     /gene="astB"
                     /locus_tag="ACHKAY_001020"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO5699735.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15031..16509)
                     /gene="astD"
                     /locus_tag="ACHKAY_001021"
     CDS             complement(15031..16509)
                     /gene="astD"
                     /locus_tag="ACHKAY_001021"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO5699736.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16506..17540)
                     /gene="astA"
                     /locus_tag="ACHKAY_001022"
     CDS             complement(16506..17540)
                     /gene="astA"
                     /locus_tag="ACHKAY_001022"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO5699737.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17537..18757)
                     /gene="astC"
                     /locus_tag="ACHKAY_001023"
     CDS             complement(17537..18757)
                     /gene="astC"
                     /locus_tag="ACHKAY_001023"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO5699738.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19203..20009
                     /gene="xthA"
                     /locus_tag="ACHKAY_001024"
     CDS             19203..20009
                     /gene="xthA"
                     /locus_tag="ACHKAY_001024"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO5699739.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20176..20886
                     /gene="ydjX"
                     /locus_tag="ACHKAY_001025"
     CDS             20176..20886
                     /gene="ydjX"
                     /locus_tag="ACHKAY_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5699740.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20891..21568
                     /gene="ydjY"
                     /locus_tag="ACHKAY_001026"
     CDS             20891..21568
                     /gene="ydjY"
                     /locus_tag="ACHKAY_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO5699741.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21583..22290
                     /gene="ydjZ"
                     /locus_tag="ACHKAY_001027"
     CDS             21583..22290
                     /gene="ydjZ"
                     /locus_tag="ACHKAY_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO5699742.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22290..22838
                     /gene="ynjA"
                     /locus_tag="ACHKAY_001028"
     CDS             22290..22838
                     /gene="ynjA"
                     /locus_tag="ACHKAY_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO5699743.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22848..24014
                     /gene="ynjB"
                     /locus_tag="ACHKAY_001029"
     CDS             22848..24014
                     /gene="ynjB"
                     /locus_tag="ACHKAY_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5699744.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPEKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23987..25522
                     /gene="ynjC"
                     /locus_tag="ACHKAY_001030"
     CDS             23987..25522
                     /gene="ynjC"
                     /locus_tag="ACHKAY_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
                     /protein_id="HFO5699745.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRTKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25522..26175
                     /gene="ynjD"
                     /locus_tag="ACHKAY_001031"
     CDS             25522..26175
                     /gene="ynjD"
                     /locus_tag="ACHKAY_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5699746.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26242..27549
                     /gene="ynjE"
                     /locus_tag="ACHKAY_001032"
     CDS             26242..27549
                     /gene="ynjE"
                     /locus_tag="ACHKAY_001032"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO5699747.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27558..28178)
                     /gene="ynjF"
                     /locus_tag="ACHKAY_001033"
     CDS             complement(27558..28178)
                     /gene="ynjF"
                     /locus_tag="ACHKAY_001033"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO5699748.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28265..28672
                     /gene="nudG"
                     /locus_tag="ACHKAY_001034"
     CDS             28265..28672
                     /gene="nudG"
                     /locus_tag="ACHKAY_001034"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO5699749.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28638..28910)
                     /gene="ynjH"
                     /locus_tag="ACHKAY_001035"
     CDS             complement(28638..28910)
                     /gene="ynjH"
                     /locus_tag="ACHKAY_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO5699750.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29146..30489
                     /gene="gdhA"
                     /locus_tag="ACHKAY_001036"
     CDS             29146..30489
                     /gene="gdhA"
                     /locus_tag="ACHKAY_001036"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO5699751.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30606..31646)
                     /gene="ynjI"
                     /locus_tag="ACHKAY_001037"
     CDS             complement(30606..31646)
                     /gene="ynjI"
                     /locus_tag="ACHKAY_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5699752.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31774..33735)
                     /gene="topB"
                     /locus_tag="ACHKAY_001038"
     CDS             complement(31774..33735)
                     /gene="topB"
                     /locus_tag="ACHKAY_001038"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO5699753.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(33740..34783)
                     /gene="selD"
                     /locus_tag="ACHKAY_001039"
     CDS             complement(33740..34783)
                     /gene="selD"
                     /locus_tag="ACHKAY_001039"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO5699754.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(34900..35451)
                     /gene="ydjA"
                     /locus_tag="ACHKAY_001040"
     CDS             complement(34900..35451)
                     /gene="ydjA"
                     /locus_tag="ACHKAY_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO5699755.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35612..37468
                     /gene="sppA"
                     /locus_tag="ACHKAY_001041"
     CDS             35612..37468
                     /gene="sppA"
                     /locus_tag="ACHKAY_001041"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO5699756.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37635..38651
                     /gene="ansA"
                     /locus_tag="ACHKAY_001042"
     CDS             37635..38651
                     /gene="ansA"
                     /locus_tag="ACHKAY_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO5699757.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38662..39303
                     /gene="pncA"
                     /locus_tag="ACHKAY_001043"
     CDS             38662..39303
                     /gene="pncA"
                     /locus_tag="ACHKAY_001043"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO5699758.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39464..39736)
                     /gene="yeaC"
                     /locus_tag="ACHKAY_001044"
     CDS             complement(39464..39736)
                     /gene="yeaC"
                     /locus_tag="ACHKAY_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO5699759.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39778..40191)
                     /gene="msrB"
                     /locus_tag="ACHKAY_001045"
     CDS             complement(39778..40191)
                     /gene="msrB"
                     /locus_tag="ACHKAY_001045"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO5699760.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40533..41528
                     /gene="gapA"
                     /locus_tag="ACHKAY_001046"
     CDS             40533..41528
                     /gene="gapA"
                     /locus_tag="ACHKAY_001046"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO5699761.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41612..42496
                     /gene="yeaD"
                     /locus_tag="ACHKAY_001047"
     CDS             41612..42496
                     /gene="yeaD"
                     /locus_tag="ACHKAY_001047"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO5699762.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42547..43401)
                     /gene="yeaE"
                     /locus_tag="ACHKAY_001048"
     CDS             complement(42547..43401)
                     /gene="yeaE"
                     /locus_tag="ACHKAY_001048"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO5699763.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43491..44237)
                     /gene="mipA"
                     /locus_tag="ACHKAY_001049"
     CDS             complement(43491..44237)
                     /gene="mipA"
                     /locus_tag="ACHKAY_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO5699764.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44673..46607
                     /gene="yeaG"
                     /locus_tag="ACHKAY_001050"
     CDS             44673..46607
                     /gene="yeaG"
                     /locus_tag="ACHKAY_001050"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO5699765.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46720..48003
                     /gene="yeaH"
                     /locus_tag="ACHKAY_001051"
     CDS             46720..48003
                     /gene="yeaH"
                     /locus_tag="ACHKAY_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO5699766.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            48150..49625
                     /gene="cdgI"
                     /locus_tag="ACHKAY_001052"
     CDS             48150..49625
                     /gene="cdgI"
                     /locus_tag="ACHKAY_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO5699767.1"
                     /translation="MIQSTRISMGLFFKYCLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            49806..51296
                     /gene="dgcJ"
                     /locus_tag="ACHKAY_001053"
     CDS             49806..51296
                     /gene="dgcJ"
                     /locus_tag="ACHKAY_001053"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO5699768.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            51339..51842
                     /gene="yeaK"
                     /locus_tag="ACHKAY_001054"
     CDS             51339..51842
                     /gene="yeaK"
                     /locus_tag="ACHKAY_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO5699769.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(51843..51947)
                     /gene="yoaI"
                     /locus_tag="ACHKAY_001055"
     CDS             complement(51843..51947)
                     /gene="yoaI"
                     /locus_tag="ACHKAY_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO5699770.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            52117..52563
                     /gene="yeaL"
                     /locus_tag="ACHKAY_001056"
     CDS             52117..52563
                     /gene="yeaL"
                     /locus_tag="ACHKAY_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO5699771.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(52520..53341)
                     /gene="nimR"
                     /locus_tag="ACHKAY_001057"
     CDS             complement(52520..53341)
                     /gene="nimR"
                     /locus_tag="ACHKAY_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO5699772.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            53438..54619
                     /gene="nimT"
                     /locus_tag="ACHKAY_001058"
     CDS             53438..54619
                     /gene="nimT"
                     /locus_tag="ACHKAY_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO5699773.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            54674..55021
                     /gene="yeaO"
                     /locus_tag="ACHKAY_001059"
     CDS             54674..55021
                     /gene="yeaO"
                     /locus_tag="ACHKAY_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO5699774.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(55043..55297)
                     /gene="yoaF"
                     /locus_tag="ACHKAY_001060"
     CDS             complement(55043..55297)
                     /gene="yoaF"
                     /locus_tag="ACHKAY_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO5699775.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            55480..56505
                     /gene="dgcP"
                     /locus_tag="ACHKAY_001061"
     CDS             55480..56505
                     /gene="dgcP"
                     /locus_tag="ACHKAY_001061"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO5699776.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            56538..56636
                     /gene="yoaK"
                     /locus_tag="ACHKAY_001062"
     CDS             56538..56636
                     /gene="yoaK"
                     /locus_tag="ACHKAY_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO5699777.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            56639..56713
                     /gene="yoaJ"
                     /locus_tag="ACHKAY_001063"
     CDS             56639..56713
                     /gene="yoaJ"
                     /locus_tag="ACHKAY_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO5699778.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(56772..57020)
                     /gene="yeaQ"
                     /locus_tag="ACHKAY_001064"
     CDS             complement(56772..57020)
                     /gene="yeaQ"
                     /locus_tag="ACHKAY_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5699779.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(57248..>57504)
                     /locus_tag="ACHKAY_001065"
     CDS             complement(57248..>57504)
                     /locus_tag="ACHKAY_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5699780.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT        14228 a        14841 c        14020 g        14415 t
ORIGIN      
        1 atgattagca aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagatcactt
       61 atttatagca atagatcgtc aaaggcagct ttttgttact ggtggtttga atgaatgtag
      121 caatgaaata cagaatttca ggtcatgtaa ctcccggcaa aaccgggagg tacgtaatct
      181 ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgctggg cggattcagg
      241 cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt
      301 gccacgcagc atccagacat cacgaaaaga atccatcttt atcgcatgtt ctggcggcgc
      361 gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt
      421 cattactggc ttcactaaac gcatattaaa aatcagaaaa actgtagttt agccgattta
      481 gcccctgtac gtcccacttt gcgtgtattt cataacaccg tttccagaat agtctccgaa
      541 gcgggatctg gctgatggtc tatagttaga gggttttttg accaaaacag cggccctttc
      601 agtaataaat taaggagacg agttcaatgt cgcaacataa cgaaaagaac ccacatcagc
      661 accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg
      721 aggacggatc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc
      781 ctgggagcct gaaagcccct gatacacgta acgaaaaact taattctctg gaagacgtac
      841 gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc
      901 aaaactcact gcgtgccggt aaccgtggtc caacgctgct ggaagatttt attctgcgcg
      961 agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat
     1021 cagcagctca cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt
     1081 tcctctcaga tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg
     1141 gcgctggctc tgccgatacc gtgcgtgata tccgtggttt tgccaccaag ttctatactg
     1201 aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc
     1261 ataaattccc cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag
     1321 ggcaaagtgc ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca
     1381 acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct
     1441 tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc
     1501 actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg
     1561 gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc
     1621 cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg
     1681 atcttctcga tccaaccaaa ctgatcccgg aagaactggt gcccgttcag cgtgtcggca
     1741 aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc
     1801 atccaggtca tattgttcct ggtctggact tcaccaacga tccgctgttg cagggacgtt
     1861 tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc
     1921 cgattaaccg tccgacctgc ccttaccaca acttccagcg tgatggcatg catcgtatgg
     1981 ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg
     2041 aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa
     2101 ataaagttcg tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg cgtctgttct
     2161 ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa
     2221 gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc
     2281 tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga
     2341 atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg
     2401 ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga
     2461 gatcggcaga ccttctggcc attctcaagg ccctgaaggc caaaggcgtt catgccaaac
     2521 tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg
     2581 ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata
     2641 tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc
     2701 ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg
     2761 accagggtga agaagggatt gtggaagctg acagcgctga cggtagtttt atggatgaac
     2821 tgctaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc
     2881 ctgcctgatg ggaggcggcg caattgcgcc gcctcaaatg atttacatag tgcgctttgt
     2941 ttatgccgga tgcggcgtga acgccttatc cggcctacaa agtcgtataa attcaatgta
     3001 ttgcagttta ttggtagata ctgataagcg aagcgcatca ggcagttttg cgtttgtcag
     3061 cagtctcaag cggcgcagtt acgccgcctt tgtaggaatt aatcgccgga tgcaaggttc
     3121 acgccgcatc tggcaaacat cctcacttac acatcccgat aactccccaa ccgataacca
     3181 cgctgagcga tagcaccttt caacgacgct gatgtcaaca catccagctc cgttaagcgt
     3241 gggaaacagt aagcactctg acggatagta ttatcgataa acgccggatg acacatcacc
     3301 tccagcgaac gatcaccccg atggcttgcg tcatccagca cttgcaggaa cagtgcctcg
     3361 ctaatctctt caccataaaa cgcgctactg aatccctgag aactgcgcag attagccggt
     3421 aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc ctcttcagcc
     3481 gcaaacctgg caacaatcgg gaaaatctgc gggaacatat gcacatgatg atggctatca
     3541 agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact ggcaagctcc
     3601 tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat ccattttccc
     3661 agcacaccat cgcgggttaa ccccggcata gctgtcaggg gcttgcccat agtaaggaca
     3721 aagtgcatcc ctatggccag actcggttca tcacgactca actgcaccgc atggtcaata
     3781 gcctgcccat tcaccagcgc cgtcgttgat gtgacaatcc cattgcgaca ggcctcgata
     3841 atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat cagtaagcgt
     3901 tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc ggcaaaattt
     3961 ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc atcgcgatcg
     4021 gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc tccgctaagt
     4081 gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc catcacttta
     4141 tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag cttacaggtc
     4201 atttctaccg cccagtctgc cggaatatta tcaatatgcc catgatgcgg gatattaacg
     4261 taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc tgcatcagag
     4321 taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc cggattttta
     4381 tacagctcaa aaagttgttt ctcgactttc tgtactacct gtgctcgtgc gccgcctttg
     4441 tagtattcgc ccatttcaat agccagcatc tctttctgct tgaagtaata cagcagataa
     4501 gaacatggca gcagatttaa cgaacgaatt aagccctcac taaatggcag atcgaaaata
     4561 ttttttacag aggacgcttt taactgccct gacgccacac catcaagcaa ttcggcaaag
     4621 cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag gccgaacaga
     4681 tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa catcttcatg
     4741 ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg ataaacggct
     4801 tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg acaaagttct
     4861 tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt aaacagaccg
     4921 cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg ttcatccagt
     4981 tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc atctttcaat
     5041 gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt atcaatcatc
     5101 cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac atccaccagc
     5161 cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag taactccggg
     5221 gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat aatttctccc
     5281 ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat aatccagcaa
     5341 caggacagat atgtgaattg tcaggtataa cgacttactg attaaattca gtcaatttct
     5401 tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata aacaaactcg
     5461 ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc atttccagtt
     5521 gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt ttgccataat
     5581 atcgttgagt ggctcgcgtc aaatattcct gtgatttggc tgacaacgtc accatattct
     5641 ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta cttttcagcc
     5701 actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg ttaatgacat
     5761 aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc aatgaagaaa
     5821 tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc gaagcgacaa
     5881 agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg atcccaacgt
     5941 tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct aacggaataa
     6001 aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg aaataacgcc
     6061 cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg atactctcag
     6121 ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt tctcgggcag
     6181 tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc cttcagtttt
     6241 tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt cattaaatga
     6301 tcctgagcgt ggaccagcac cagactcact ttcatcttaa cttcgcccgc atcgccttca
     6361 atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg atccatcatg
     6421 gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata cgccaggctg
     6481 cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc ttcagcttct
     6541 gtttgcgtat caggaatatt atcgagatcc atcatacatc gttcctctct cttcttaccg
     6601 gcaagattac ccgtaccggc atcgattaaa atttcagggc gttagcgata tcttcttcgc
     6661 tctcttcttt atcaatcgca ttttgtgctt tgttagccac cacaacaaag ggcagataaa
     6721 ttaaggttgc gatgccaagg ttgaagagtg cgaccagcaa tgcggcgacg ctaccgttgg
     6781 tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg gtcaccggag
     6841 gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt tgtaccagta
     6901 caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga atcggttcgt
     6961 taatctggaa gatgccggac ggcagcgcca gttttgccac ctgacgataa tcagcacggc
     7021 gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc ccaaggaaaa
     7081 taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct gccagcgccg
     7141 cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac ggcgtcataa
     7201 tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac cagagcagtg
     7261 gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt ggggttgaga
     7321 tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc caggcaataa
     7381 tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa cgcgatactg
     7441 aagcaggtac gctgtcgggc agtttaatga cccaattgcg gcggacaata aaggtaaaca
     7501 tttctgccac caccaggcca ataataatcc cggagatgat attcgcccca cctaaccagt
     7561 ttgcgccaac cgcataggcc tcaccgacac tatatggggt gacggtcata aatgctgcaa
     7621 cggataacaa ccccgccgcc agcgcatcga ctttacgctc ttctgccagc gccatgccaa
     7681 taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg ttgccgccaa
     7741 tacctttcag gccattaagt gtttcaatgg ttgaggcgtc gaggcgaata cctaaggaat
     7801 aaaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca aacatggccc
     7861 ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta acgtgtggct
     7921 gctttcctat tttaactgca aaagggagga gtaccttttc aagcgatgca ataacattac
     7981 tcatagaaaa atacccttaa aaaccgcaat ttaaatattg cggtattgat ttatgaaata
     8041 actctttgac gggaaaattt aaaataaatt aatttgctgc ggctttttta atcgctgcaa
     8101 ccgcagcctt aagcacgcct aaaccatcga ctttgccata aagcagcgag tcaattactt
     8161 caaccggttt gttgggtaac aaacgctgga tttcgggcaa catataagca atctgcggcc
     8221 ctaataacac gacatcggca ttctgacctt tttcaccagc cagtgtttcc ggaaatgctt
     8281 caataatgac cggaacttca tatttttctg cctgtgcgcg catttttgat accagtaaag
     8341 aggtagacat gcccgcagaa caaaacagat aaatgtgttt cttttccata aaactgccct
     8401 catcgacgat tatctgtcag ccagacactc cgcaagcctt aacctgcttc catgctctgg
     8461 gtaacttgcg aaaccaacat gacttttttt gtggctgaaa ggagtatacg gtatagacct
     8521 gagcaatagt atttctttga ccttataaat tgtttctcct tgacctgtgg ttatgaccct
     8581 cttcacattt cgggcaaata attcgcgaag ataattaaga actcaggcca aaaaaaaccg
     8641 gcgcaatggc cggtttccgt tgttactcaa gctttcagac gaattgatta cttcgcagcc
     8701 tgtggatcag tgtcgtattc agcacaggtc tgataaccgg agttgatgac atgtccggta
     8761 tcatctaacg cgacaaagta ggtttctgct ttaccatcac gttgacccag gatgtaggtc
     8821 tggcaagtac cgcgagcatg gatcatgctc acttcagacg aaggtttacc cgcaatttgt
     8881 gcaacctgcg cccggctcat gccttttttg acgtctttca ccacaggctg tacaaactgg
     8941 tctttggtac gatcataagc cgtacaaccc gccagcatgg ttaataccgc cgctgcactc
     9001 agaattcctg ccatattctt gttcatattc cgtcctcttg tttatcagcg tgttagataa
     9061 gcctggaata cattgggcgc tttttcaagc ccgtgaacga aacggctccg ctttcagagg
     9121 attcctgtat gacgttttaa ccaccattca gcccgctgtc gcttgtcgtt tcagtagcaa
     9181 cgggttagct ttaaggaagt tttgtctttt ctgtctggag gggttcaatg acattgcaac
     9241 aacaaataat aaaggcgctg ggcgcaaaac cgcagattaa tgctgaagag gaaattcgtc
     9301 gtagtgtcga ttttctgaaa agctacctgc aaacttatcc gttcattaaa tcactggtgc
     9361 tcgggatcag cggcggtcag gactccacgc ttgccggaaa gctgtgccag atggcgatta
     9421 atgagctgcg ccaggaaact ggcaacgaat cactgcaatt tattgccgta cgcctgccct
     9481 atggtgttca ggctgacgaa caagattgcc aggatgccat tgcctttatt caaccggatc
     9541 gcgtattaac cgttaatatc aagggcgcgg tattggctag cgagcaggca ttgcgggaag
     9601 caggcattga actgagcgat tttgtccgtg gcaatgaaaa agcgcgtgag cggatgaaag
     9661 cacaatatag cattgcgggt atgaccagcg gtgtcgtggt gggcaccgat catgcagcag
     9721 aagccattac cggattcttc actaaatatg gtgacggcgg tacggatatt aatccgctgt
     9781 atcgtctcaa caaacgccag ggtaaacagt tgctgacggc attaggttgc ccggaacacc
     9841 tttataagaa agccccaacg gccgatctgg aagatgatcg cccttctctg ccagatgaag
     9901 tggcactcgg cgtgacctat gacaatatcg acgactatct ggaagggaaa aacgtacctg
     9961 aacaggtcgc cagaacaata gagaactggt atctgaaaac cgaacataaa cgccgtccgc
    10021 caattaccgt tttcgatgat ttctggaaaa agtaataatt tgcacataat ggtttgtgcg
    10081 aaaaagagta atttgttcac gccggatgcg gcgtgaacgc cttattcgac ctacaaaact
    10141 atgcaaattc aatatattgc aggagtcgaa taggcctgac aggcgtagca cgtcagacgg
    10201 tgtaaccttt gtcatcgacc cgcctctttt ttaatcgctt cccgcctgtt acactggata
    10261 gataaccagc attcggagtc aacagtggta cggcgtttaa cttctccgcg gctcgaattt
    10321 gaagctgcgg caatttatga atatcccgaa catttacgtt cattccttaa tgacttaccc
    10381 acccgaccag gggtgtatct gtttcatggc gaaagtgaca ccatgccgct ctatatcggc
    10441 aaaagcgtta atatccgcag tcgggtgctt tctcatttac gcaccccgga tgaagccgcc
    10501 atgctacggc aatcccggcg aattagctgg atctgcaccg caggcgaaat cggcgctctg
    10561 ctccttgaag cgcgattaat caaagaacaa cagccgctgt ttaataaacg gttgcgccgc
    10621 aatcgccagc tctgtgccct gcaattaaat gaaaagcgcg tcgatgtggt gtatgccaaa
    10681 gaggtggatt tttcacgagc ccccaacctg tttggcctgt ttgccaatag gcgcgcagct
    10741 ttgcaagcat tgcagagcat cgctgatgaa caaaaacttt gttatggcct gctgggactg
    10801 gaaccgttaa gtcgcggtcg tgcatgtttt cgttcagcgc taaaacgttg cgccggagca
    10861 tgctgcggta aagagagcca tgaggaacat gcgctacgct tgcgccagtc tctggagcgt
    10921 ttgcgggtgg tgtgttggcc ttggcaaggg gcggtggcgc tgaaagaaca gcacccggaa
    10981 atgactcaat atcatattat tcaaaactgg ctgtggctgg gggcggttaa ttcgctggaa
    11041 gacgcgacaa cgttaattcg gacacccgcc gggtttgatc acgacggtta taaaattctt
    11101 tgtaagccgc tgctttccgg taactatgaa attactgaac ttgatccggc gaatgaccag
    11161 cgagccagtt gatttcactg aacaacagtt taccttgcgg ttgtaaaagc cgcagggtgt
    11221 gcctgccatc gaaccagcac gcgccctggt cggtagtgag cagtttgtcg cccagttgcc
    11281 acgccccgtt aatcacaaac actaccccgc cgcgtgagcc aaaggtagta aacgtacgtt
    11341 cggcaattct gactttcgcc ttacagacat ccaggcgcgt cataatattg aaatccatcg
    11401 acatctgccc tgccgtcagt ttcgctttta ccacctggtc cgccgcaaag gcaaaaggct
    11461 gtaacggttt taaggtatgg ttaaagtggt ctgcgctttc aaggaacatc tcgccgcctt
    11521 ccagcaacgt cactatcctt tccataccgg gaaacaaaga aaactcccca ttcgccgcga
    11581 tggatgcaat gctggcacgc cagtaaaaat cacgttttgc cggtgggaaa gtgcaaattt
    11641 cgcgcgtttc accggcagca tttcgccaca gattcaccga cattttacgc atatcaaagt
    11701 attccatact cgctcccggc ccgttctttt cattgttatc gttcaggtaa ccgtggatgg
    11761 ttatcggcaa ctcgggtgat ttacgttagt ggtgatcagg ctttttttac aactgtttga
    11821 gaagagaaaa gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac
    11881 aagaccggcg gtcttaagtt ttttggctga aagattattc agcagttgca ggcattttac
    11941 cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat tgctttttct
    12001 gttccggcgt caggatgttg taaattttgt tctgggtttc catgtgcgcc agcatgttag
    12061 ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat
    12121 cgctggcaac gatgtcatgc attgcgcggc gttcttccag cggcggacgt ttcatctggt
    12181 cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc aggttcaggt
    12241 ctttgaacat catgtcctga tgcggaccga acttgccttt gtggtgcata atcggcttcg
    12301 cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc gcgccaagag
    12361 ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc cttcctttca
    12421 gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac tttactgact
    12481 ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc
    12541 caattatgga gaaaaaaaga aaatttgatg gagagtgatg agagaatatt acaacacgat
    12601 gattttgcag agattatgaa gaactatacc ggatgactgg tgataaataa agcaaataac
    12661 caggattaat ctgtattaat ttataagaaa gcaacttaat acccgcagag tgatttctgc
    12721 gggtaagtat tagcttattt tttcgagcat taatcccgcg cgtaatccca acgctaccaa
    12781 cggattaggg aataacacat actctacatc atgggttacg gtaaaacgtt cctctccgtc
    12841 ctgcgccagc aatgttcctt tctcaaacgg cataaaattc agcgtgtcac ttgccatatg
    12901 catttcgaag gacggcgagt ggcgagtaat ttgcgaaacc acccgataac ggagcggcgg
    12961 tgttctcacg ataccgacac tctcaccaga tagcagcgca gcaattgcgc tggcagttac
    13021 tgcaaactgg cgaagatcgt tttgcccaaa gggcaacgct ttgccaagtt ccagcgtaca
    13081 ggccagcgcg ccaaaatgtc tggcgctgaa atgggtaaac gtaccaccag gttcctgatg
    13141 gaacaccagc gcctccagcc ccgccgcacc cagccacgtc agaaatttct cgtcccaggg
    13201 aatgtcgcgt tgcggtaata caccgaactg cggatgcaag gagccacgaa ttgcggtatg
    13261 tagatcaagg tgccagcgca cagattcttt gccctggtca taaaaatctt ccaggcactg
    13321 ttccagttcg cgcgcccgac aggtttctcc gctttcagca aatagctgcc aacgaccgcc
    13381 aaacattcga ttcatatcgc tatggcaata acgtttccct tgcttcagcg caggaggatt
    13441 cccgaggatc accagcaacc gccaacgtaa cgggatctcg ccgtgagata tcgcgccaag
    13501 caacgcgtcc agcatctcca caggtgccgt ctcattaccg tgtattcccg ctgaaatcac
    13561 cagtgcgcct tgcggtggcg ttaatggcgt cagttccagc acaccatcgc ccagccagcg
    13621 ccagcgaacg ccgttgattt ctcgctcggt gataaccggt tttttacccg ttaaggtcag
    13681 agcaagaaaa ttatccattg ccccctccct cgcgctggaa cggataaacc gaaccgagat
    13741 tcagtaattg gctcaataca tccagtgctt cccgcccttc gcgcagcaat tgcgggtcgg
    13801 ccagatcggc agcagtaagg cgatcgcggt agtaacgatc cacccagtca ttgagcgcat
    13861 taaacagcgt atcgttcatc atcaccgccg gattcaccgc ccggcgttct tcttctgtca
    13921 ataccacccg caaccgcagg cacgccgggc cgccgccatt cgccatgctt tcacgtaaat
    13981 caaagacttt tagttcgcta atcgggttgt cagcggcaag gagttcattg agataacccc
    14041 atactccggc gtgttcccga cactcctgag gcagcaccaa catcatagaa ccatcatcgc
    14101 ggctcaacag ttggctgtta aacagatagg tagacaccgc atccgacacg gaaacctgag
    14161 ttgccggaac ttctatcgcc ataaaaccgt tcacccgcgc acgcaggttt gccagtaact
    14221 gtgactggcg agcgaacgcc tgttggtggc aaaacagcac ctggcggtta ctcacggcaa
    14281 tcacgtcatt atgaaaaacg ccctggtcga taacgtccgg gttttgctgg gcgaaaatca
    14341 cctgttgggg attcacctga ttcagccttg ccaccgcctc gctggcttcg cgagtctgtc
    14401 gcgccggata acgggaaggc cgggtatcat tgccttcttc tcgcccgtag acaaaaagtt
    14461 gcatacccgg ctcaccgtaa tgaccgccga gacgattgtg gtttgccgcc ccctcatcac
    14521 cgagcaacgc tacctgcggc aacgccgaat gaacgctaaa tttctcttcg tcgttaaaaa
    14581 tcgcttttaa aagtgattcg gtgacgtgcg cttccagcga gcggtgaaat ttattgttca
    14641 ggttggcaac ggtgagatgc actttgccat ccagcgtatc ggcagatggc gcgatcgttg
    14701 ccgcattggc tacccacatt ggcgaagcgg aactgacgct ggaaagccag tgcggtgcct
    14761 ggcgtgcaac tttttccagt acctgctcat cgctaccact gaatcccaac tgacgcagca
    14821 ccggaataaa cggacgctcg tgcggcggga tcacggcctg ggggaatccc gcatcggcaa
    14881 gggctttcat tttcagtaag ccctgttttg ccgcctgtcg cgggttagac acctgaaaac
    14941 ggtgacgggt agaggcttca ttaccaaacg acaggccagc gtaatgatgc gtcagcccta
    15001 ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc acctcatcgg aaaaatccag
    15061 cccggggtta agcgttgcgg gcaaagttaa cgagtccgac tccaggctcg ccataggcca
    15121 tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg ttaccggatg caccaatgcc
    15181 gccgaatggc gcggtactgg cagcaccagt aagcggtttg ttccagttaa caatccccgc
    15241 ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg ggggaaacca gaccgcaaga
    15301 gagtccgaag cgagtgttat tcgccattag aatcgcttcc tcgaaagaat cataacgcca
    15361 gacgcgcaat aacggtccga acacctcttc atctggtacg ccagcaacgc ctgtcatttc
    15421 aatgatcccc ggcgtcagca acgatgtctc tgattgtaat aagcgcggcg caagcagggt
    15481 tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc acctgctgtg cggcctgttc
    15541 agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc cagttgcccg gcgttaatcg
    15601 ctggctgacg gcaaccagac gagcaagaaa cgcatcgccc tgcgctccgc ttttgagcaa
    15661 taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca aacgccgact gaatggtcag
    15721 atggacagcc gcgtcgatat ccgccacctc atcgataatt agcggattat taccgcccat
    15781 ctcaagggcg agaattttct ccggctgacc ggagagctgg cgatgcagct ggtagccggt
    15841 attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc agcgcactca gcgcctgacc
    15901 cgtttcacgc ccgccctgca ccaggttcag tacgcccggc ggcaagccag cctgctgcca
    15961 taaacgcatt accgcttcgc cactccacgg tgtcagttcg ctgggtttaa agataatggt
    16021 gttacctgcc agcaatgccg gaacgatatg tccgttcggc aaatgaccag ggaaattata
    16081 cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc aggctcgccg caccgtccgg
    16141 catttcacta cgctgctcgc cggtacgaac gtgatacgcc ttaattgata tcgcgatttt
    16201 attgatcatc gccgtcactt cggttgccgc ttcccagcgc ggcttacccg tttctctggc
    16261 aataatcgcg gttaattcgc ctttattcct ttccagcaat ccggcaaagc gttcgacaac
    16321 gacctgacgt tcagccaatg agagccgcgc ccagcgcgga aacgccgcac gggctgcccg
    16381 acaagcctgt tcgacctgag cggcatcggc atcattgcct tgccataaca cctcgcccga
    16441 taccggatta cgcttcacac gcgatgcgcc ctggcccgtt atccagtcac cgttaatcca
    16501 taaagtcatg ctgttttctc ctctgcgcac aggcgcacca gacgaacgcg atcccctgcg
    16561 tggcatttga gggcatccag ttgtgcggcg gttaaaatca aacgctcggt tgccggatcg
    16621 gtacgcgcca gcaccacgcg gaaatggtga taattttcat tggcgaccag gcaggccgga
    16681 aaatcgccct gcgcaggctg cccttctgcc acttccacca gccgactttt acgaatggcg
    16741 cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt cgaagatgtc gatatagttg
    16801 cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag gcgcggtttg cggatgtacc
    16861 tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg tatagatcgg atgtttcggc
    16921 atcagttctg caataaatgc cttttgcccg gtgccgcaga gaaaatcggc gcggctaaaa
    16981 tccatcgaaa agaagcgttt accgaggctt tgccagaacg gtgaatagcc gtgttcgtca
    17041 atcaccccgc gcatttcagc aaccactttg tcattaaact tgtcgcgaaa agccgccata
    17101 aacataaagc gcgatttcga cagcaaatag ccgttgccct ctttgcgcca gtccgggtcg
    17161 agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt tactgagaaa cagcgtcggc
    17221 aatgcgttat agacattcag ctcttttgag gcgtgaacca acgtgccgac gcgatagttg
    17281 taccagggat cgttcagccc aaccgccacc tcaatggcac aaatccccgc cacggtgcct
    17341 gtctcgctat cttccagcac gaacacatag ccctgctcac ttttgggcag ttcgccttgc
    17401 caggttttga ttgccctttc gatacgcgcc gaaagcgtgg cttcattggc gggaagcgac
    17461 gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg ccgagacatc tgatcgctca
    17521 acgggacgga tgaccatcat gatgaacctc ggctaacaaa gtgttcgcaa gcagctgcaa
    17581 agcgatccag tccggtcgtc acctcttctt cgctgacatt gagcgcaggc gcaaaacgca
    17641 ccacgttgcc acccgcaatc agtaccatca cgcctgcttt cgccgcttcc tgagagatct
    17701 gtttcgcttg cccggcgtaa tcggcattca gtacacagcc aatcagcaaa cctaagccgc
    17761 gaacttcact gaacaaacca tagcggtgat taatagtatt aagacgctca acaaaccagt
    17821 cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt gatgagctcc agcactttgc
    17881 ctgccaccgc cgaggccagc ggattaccgc cataggtggt gccatgagtg ccaacggtca
    17941 tcacgcgggc gcactcttcg gttgccaaca acgcaccgac cgggaaaccg ccgcccagcg
    18001 ctttggcggt agttaacaga tcaggcgtca cgccgtagtg catataggca tacagttccc
    18061 cggtgcgccc gacgccggtt tgtacttcat caaaaatcaa cagcgcattg tggcggttac
    18121 acaattcacg cagaccttgt aaaaacgcgt tgctggctgg caccacaccg ccttccccct
    18181 ggatgggttc gacaatcacc gcacaggtag agtcgtcaat cagcgcgctg gcagagttaa
    18241 tatcgttata tgcagcatga cgaatatccg ccggcagtgg cgcaaaatcc tgtgaatagg
    18301 ctggctgccc acccgcactg acagtaaaca gcgtgcgacc atgaaacgca tttttgaacg
    18361 ccacgatgcc gctcttatgg ctgccgtagc ggtcgtgagc gaatttacgc gccagtttta
    18421 gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa gacgcgatcg gcaaacgtgg
    18481 cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt ggtgtaaccg ttgccggtat
    18541 gccagaactt actcgcctgt tcgttcagcg cttcacgcag ttccggatgc gcatggccca
    18601 gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc tttccccagc tgatcccaca
    18661 agcgcgaacc ttcgccacgt accggtataa agggtgccgg agcgtaaaca ggtatcatcc
    18721 attcatcaaa gttttcacgc gtaattggct gagacatagc gacctctaca gtaaataatt
    18781 cgttatttat atgttaacaa taagtaatgt ttgcgctgta aatgtagatt gcagggttcg
    18841 tgccagccag tgataaaagt gcataaacgg cggaggctaa ctggaaatca aggagttata
    18901 accaaaccat atgcatttaa agtgcatata aagtgaatac gtttgcgatg tgggtgaata
    18961 aaaagaataa aaaacgcaat gttatgcaga agtaaaatat aattctggaa ttgtgatcat
    19021 tgacgaaatt tactggaaat tactgcgcca ttctgacgca gcgcgcacca aaagcgggca
    19081 ttttttgcgc catcgttgac atcattaaca accatcgatc aaatcactta acaacaggcg
    19141 gtaagcaacg cgaaattctg ctaccatcca cgcactcttt atctgaataa atggcagcga
    19201 ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc cagacctcac cagcttgaag
    19261 ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca ggagacaaaa gttcatgacg
    19321 atatgtttcc gctcgaagag gtggcgaagc tcggctacaa cgtgttttat cacgggcaga
    19381 aaggccatta tggcgtggcg ctgctgacca aagagacgcc gattgccgtg cgtcgcggct
    19441 ttcccggtga cgacgaagag gcgcagcggc ggattattat ggcggaaatc ccctcactgc
    19501 tgggtaatgt caccgtgatc aacggttact tcccgcaggg tgaaagccgc gaccatccga
    19561 taaaattccc ggcaaaagcg cagttttatc agaatctgca aaactacctg gaaaccgaac
    19621 tcaaacgtga taatccggta ctgattatgg gcgatatgaa tatcagccct acagatctgg
    19681 atatcggcat tggcgaagaa aaccgtaagc gctggctgcg taccggtaaa tgctctttcc
    19741 tgccggaaga gcgcgaatgg atggacaggc tgatgagctg ggggttggtc gataccttcc
    19801 gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt tgattaccgc tcaaaaggtt
    19861 ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc cagccaaccg ctggcagaat
    19921 gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat ggaaaaaccg tccgatcacg
    19981 cccccgtctg ggcgaccttc cgccgctaat ttagcagctc tcctggctca aactgggtca
    20041 ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa tgatttgttg cctgccgtcc
    20101 tttgttatac cgtcactgcg tttttagttg tctgaccact tctctattat caagtttgat
    20161 ataggaaact ccacgatgaa cgctgagcgt aactttctgt ttgcctgtct tatttttgcg
    20221 ctggtcattt acgctatcca cgctttcggt ttattcgatc tgctcaccga tttaccccac
    20281 ttacagacac tcattcgcca gagcggattg ttcggttata gcctctatat tctgttattc
    20341 atcattgcca ccctctttct gttaccagga agcatactgg tgatcgctgg aggaatagtt
    20401 tttggcccgc tcttagggac actactctca ttaattgccg caacgctggc ctcctcgtgc
    20461 tcattcctgc tggcgcgctg gctgggacgt gatttactgc tgaaatacgt gggccatagc
    20521 cataccttcc aggccattga aaaaggcatt gcgcgtaacg gtattgattt tcttattctg
    20581 acccgcttaa tcccgttgtt tccttacaat attcaaaatt acgcttacgg attaaccacc
    20641 atcgccttct ggccttatac ccttatttcg gcactcacga ccctgctcgg tattgttatt
    20701 tataccgtga tggcaagcga tctcgccaat gaaggcatta cgctgcgctt tattttacaa
    20761 ctctgtctgg cgggcctggc gctgtttatt ctcgtccagc tcgcaaaact ctacgcccga
    20821 cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac ttactcaccc aaaaaatgaa
    20881 ggatagaacg atgtcgcaac attattcagt gtcatggaaa aaaggactgg ctgcactctg
    20941 cttactggct gttgccgggc ttagcggctg cgatcaaaaa gagaatgcgg cggcaaaagt
    21001 ggaatacgac ggactttcga acagccaacc gctgcgcgtc gatgccaata accatacggt
    21061 aaccatgctg gtgcaaatta atggtcgttt cctcaccgac gacactcgtc acggtattgt
    21121 gtttaaagat ggctccaacg gacataaatc gctgtttatg gcttatgcga ccccgaaagc
    21181 attttatgaa gccctgaaag aggcaggtgg tacgccgggc gaaaacatga cgatggataa
    21241 taaagaaacg actcatgtca caggcagcaa actggatatt tcggtcaact ggcaaggggc
    21301 ggcaaaagcg tattccttcg atgaagtgat tgttgacagt aatggcaaga aactggacat
    21361 gcgctttggc ggtaatttaa cggcagcaga agagaagaaa acgggttgcc tggtgtgtct
    21421 ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac acttatggtg cggtggaaaa
    21481 acgcggcgaa gtgaaattca aaggcaatgc ctcagttctc ccggcggata acacgctggc
    21541 aacggttacc tttaaaatca ccgaataaag ccaggataaa ggatgatgaa gatacaatcg
    21601 cgtaaaatct ggtactaccg tataaccctc atcatcctgt tgttcgctat gctgctcgca
    21661 tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca gcgttgcggc gtttgccgcc
    21721 gtggaccaac agggtataga acgctttatt cagtcttacg gcgcactggc ggcagttgtc
    21781 tcgttcttgt tgatgatttt acaggccatt gctgcaccgc tacctgcgtt tttgatcacc
    21841 tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct tgctgtcgtg gaccagttcg
    21901 atggccggcg cggcgctgtg cttttttatc gccagagtga tgggccgcga agtggtggaa
    21961 aaattaaccg gcaaaaccgt gcttgacagt atggacggct ttttcactcg ctacggcaaa
    22021 cacaccatcc tggtctgtcg gttattgcct tttgtccctt tcgatccaat cagctatgct
    22081 gccggtctga cttcaatacg ttttcgctcg ttttttatcg ccaccgggct tggtcaatta
    22141 ccggcgacta ttgtttattc ctgggcgggc agcatgttaa caggcggtac tttctggttt
    22201 gtcaccggac tgtttattct gtttgccctg accgtggtga tttttatggc gaagaaaata
    22261 tggcttgagc gccagaagag gaatgcctga tgggtttacc gccgcttagc aaaattcctt
    22321 tcattttacg tccgcaggcg tggctgcatc gtcgccatta cggcgaggtg ctaagcccga
    22381 ttcgctggtg ggggcggatc ccgtttatct tttatttggt gtcaatgttt gttgggtggc
    22441 tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc gcttgtcagc gcgcgcattg
    22501 cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag tatgaaagtc gccgagcgta
    22561 ccggcagcac cgataaactg ctggcagtgg ctgactggcg gcaaagcccg ctcttcagcg
    22621 atgaagaacg gctggcgctg gagtacgccg aagccgcaag cgtaacgccg ccaacggtcg
    22681 atgatgccct gcgcacccga ctggctgcgc attttgacgc tcaggcgctc accgaactga
    22741 cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa ttctgccatg gacattcccg
    22801 ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg agagaagatg cgccattgtg
    22861 ggtggttgct gggattgtta tcgctgtttt ctctggcaac acatgccagt gactggcagg
    22921 aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa cgcctggggc ggcgataccg
    22981 caattaaccg ctatctcgac tgggttagcg gcgagatgaa aacccattac gctataaacc
    23041 tgaagattgt tcgtctggcg gatgccgcag acgcggtgaa gcgcattcag accgaagctg
    23101 ctgccggacg taaaacgggc ggctcggtgg atctgctctg ggtgaacggc gaaaacttcc
    23161 gcaccttaaa agaggccaat ttattacaaa cgggctgggc ggagactctg cccaactggc
    23221 gctatgtcga cacacagctg ccggtgcggg aagatttttc agtgcctaca gaaggggctg
    23281 aatcgccctg gggcggcgca caactgacgt ttatcgcccg ccgcgatgtt acgccacagc
    23341 caccacaaac gccgcaagcc ttactggagt ttgctaaagc caatcccggc acggttacct
    23401 atccgcgccc accggacttt accggcacgg cgtttcttga acagttgctg attatgctga
    23461 cgcccgatcc cgccgcgtta aaagttgccc cggagaaagc cactttcgcc cgcgtcactg
    23521 cgcccttgtg gcaatatctt gacgcgctac atccctattt atggcgcaaa ggaaaggatt
    23581 tcccgccttc gcccgcgcgg atggatgctc tgctgaaagc cggcacattg cgcctgtcgc
    23641 tgacctttaa ccctgctcat gcccagcaaa aaatcgccag cggcgatttg cctgcaagca
    23701 gttacagttt tggttttaac caggggatga ttggcaacgt gcatttcgtc accattcctg
    23761 ctaacgcaaa tgccagtgct gcggcgaagg tggttgccaa cttcctgctc tcacccaatg
    23821 cgcaactgcg taaagcggat cccgctgtct ggggcgatcc ttctgttctc gatccgcaaa
    23881 aactgcctga tgggcagcgc gaaacattgc aatcaagaat gccgcaagat ctgccgccgg
    23941 tactggctga accgcacgca ggttgggtaa atgcgctgga acaagaatgg ctacaccgtt
    24001 acggtacgca ttaatctttt tgctgtgggc gatggtggcg gtgatttatg caccactgat
    24061 cccggcagct ctcacgctga tatcgcctgc tttgtcgttg acacactggc aggcgttatt
    24121 tgccgatcca cagttaccgc aggcattact ggcaacgctg gtgtcgacaa ccatcgcggc
    24181 ggtcggggcg ttgttgattg ccctgctggt gattgtggcg ctgtggcctt ggccgaaatg
    24241 gcagcgtatg tgtgcccgtc tgccgtggct gctcgccatt ccccatgtgg cttttgccac
    24301 cagcgccctt ctgctctttg ctgacggagg gctgctttat gactatttcc cgtatttcac
    24361 tccgctaatg gacaaattgg gtattgggct gggcctcacc cttgcagtga aagaaagcgc
    24421 atttctgctg tggatcttag cggcagtatt gagcgaaaaa cggctgttgc agcaggtcat
    24481 tgtgctggat tcgctgggct acagccgctg gcaatgcctg aactggctgc tcttgccctc
    24541 cgtcgcacct gcactggcaa tggcgatgtt ggcgattgtt gcctggtcgc tttcggtcgt
    24601 ggatgtggcg attattcttg ggccaggtaa tccaccgacg ctggcagtaa ttagctggca
    24661 gtggttaact cagggcgacg cggatcaaca aacgaaaggc gcactcgcca gtctgctgct
    24721 gatgctgtta ctcgccgcct acgttttgct gagctatctg ctatggcgca gctggcggcg
    24781 cactattccc cgcgtagatg gcattcgcaa gcctgccaca cctttattac caggcactac
    24841 gctggcgagt tttttaccct taaccggtgt gctgtgtgtg gttctgctgg cgatcctcgc
    24901 ggatcagtcg acgatcaata gtgaagcact catcaacagc ctgacaatgg ggctggtggc
    24961 gacattcatc gctttgctcc tgttactgct gtggctggaa tgggggccac agcgtcgcca
    25021 gttgtggctc tggttgccca ctctattgcc cgctctgcca ctggtggcgg gccagtacac
    25081 gctggcgcta tggctgaacc tggatggaag ctggacggcg gtggtctggg ggcatctgct
    25141 gtgggtaatg ccgtggatgc tgtttatcct gcaacccgcc tggcagcgca ttgattcacg
    25201 gttaattttg attgcgcaaa cactgagctg gtcgcggacc aaaatcttct tttacgtgaa
    25261 atgtccgctt atgctacgcc ctacgctgat tgccttcgcg gtgggatttt cagtcagtat
    25321 tgcgcagtat atgccgacgc tgtggctggg cgcggggcgt tttccgacgc tcaccactga
    25381 ggcggtggca ttaagcagcg gcggcagcaa tggtattttc gccgcccagg ctttatggca
    25441 actgctatta ccgcttatta tttttgccct gaccgcgtta gtcgcaaaat gggtaggtta
    25501 tgtcagacaa ggactccgct aatgctctgc gtgaaaaatg tttcgctacg tttaccagaa
    25561 agccgcttgc tgacaaacgt taactttacg gtggataaag gtgacattgt cacgttaatg
    25621 gggccgtctg gctgtggaaa atccactctg ttttcatgga tgattggtgc actggctgga
    25681 cagttttctt gtacaggtga gctatggctc aatgagcaac ggattgacat gctacccacc
    25741 gcacagcgtc agattggcat tcttttccag gatgcactgt tatttgacca gttcagcgtc
    25801 gggcaaaatt tactgctggc gctaccgtcg acgctcaagg ggactgccag gcgcaatgcc
    25861 gtgaaagacg cgcttgatcg agcgggactg gctgaaactt atcaccagga ccctgcgaca
    25921 ttatcaggcg gtcagcgagc gcgggtggcc ctgctgcgcg cccttctcgc tcagccaaaa
    25981 gcgttactcc tggatgagcc attcagccgt cttgatgtgg ctctgcgcga taattttcgc
    26041 cagtgggtgt tcagcgaagt tcgcgaactg gcgatccccg tcgttcaggt aacgcacgat
    26101 ctccaggatg ttcctgctga tagttctgtt ctggatatgg agcagtggtc agaaaattac
    26161 aacaaactgc gataacgcaa agtttttctc aatgcgtcag ttcagaatgg cgcactcaaa
    26221 actacaatgt cgggattttc gatgaaacgt gtttctcaaa tgaccgcgct ggcaatggct
    26281 ttagggctgg cttgcgcttc ttcgtgggcc gctgaactgg cgaagcctct tacacttgac
    26341 cagcttcaac aacaaaatgg caaagcgata gatactcgcc ccagcgcgtt ttataacggc
    26401 tggccacaaa ccttaaatgg cccttctggt catgaacctg ccgccttaaa cctctctgcc
    26461 agctggctcg acaaaatgag caccgaacag ctcaacgcgt ggatcaagca acataacctg
    26521 aaagccgatg ccccggtggc gctgtacggt aatgacaaag atgtcgacgc cgtcaaaacg
    26581 cgactgcaaa aagcaggttt tacgcatatc tccattctga gtgacgcgct aagcgaacct
    26641 tcccgtctac aaaaactgcc gcactttgag cagctggttt atccgcaatg gctgcacgac
    26701 ctgcaacaag gtaaagaggt tacggcgaaa cctgccggtg actggaaagt cattgaagcg
    26761 gcctggggcg ctcctaagct ttaccttatc agccatattc ccggtgctga ctacatcgat
    26821 accaacgaag tggaaagcga accgctgtgg aacaaagttt ctgatgaaca actaaaagcg
    26881 atgctggcaa aacacggcat tcgccatgac accacggtca ttctgtacgg gcgtgacgta
    26941 tacgctggag cgcgtgtggc gcaaattatg ctctacgcag gcgtgaaaga tgtgcgtctg
    27001 ctggatggcg gctggcaaac ctggtccgac gcgggactgc ctgttgagcg aggaacgcca
    27061 ccgaaagtga aagcggaacc ggatttcggc gtgaagatcc cggcacaacc gcagttgatg
    27121 ctcgatatgg aacaggcccg tggactgctg catcgccagg atgcatcgct ggtgagcatt
    27181 cgttcgtggc cagaatttat cggtacgacc agcggttaca gctatattaa accaaaaggt
    27241 gaaatagccg gagcacgttg gggacacgct ggtagcgact cgacgcatat ggaagatttt
    27301 cataacccgg atggcaccat gcgcagcgcc gatgatatta ccgctatgtg gaaagcgtgg
    27361 aatatcaaac cagagcagca agtttcattc tactgcggca ccggctggcg cgcgtccgaa
    27421 acctttatgt acgcacgcgc catgggttgg aagaatgttt ccgtgtatga cggcggctgg
    27481 tacgaatgga gcagcgatcc aaaaaatccg gtagcaaccg gtgaacgcgg cccggacagt
    27541 agcaaataac attgcaatta ctgacgctgg tgcgacttca gcgtcagata cccactccac
    27601 acccgcgtaa aggtcgtcat ccagcacaac gcaccgaaga tccacgcaaa ccacgcaaac
    27661 catgcaggga ataaacatcc cagcacaaac agtaagatcg tttctgtgcc ttcggttaac
    27721 ccgcccagat aataaaacga tttatgcgca taaccgggat tatcaatctg atgtttcgcc
    27781 gccagcgcag caaaagcgag aaaactgctg cctgtgccga taaacgcaaa cagcagccag
    27841 ccgcctgcca gggcgttttg ctgcggtgcg gcgaggataa acccaaaagg taccagcgcg
    27901 taaaaaagaa aatcgagaga aatatcaaga aagccgcccg cgtcggtaag ccctctgcgc
    27961 cgcgccagcg caccgtccag accatcaagc agcctgttca acaaaatgac gacaagcgcc
    28021 gccagatacc agcccagcgc cagaaaaggc agtgccagca cgccgatggc aaatcccact
    28081 aatgttaaac catctggcgt aatgcccggt ttatcaagaa cccgcacgca ctgatgcaac
    28141 aacggtttaa tccggggatg aagatggcgg tctagcacag gcactcctta aatataaagc
    28201 ctttctgatt gagcaacagt gcggatatta tggcattttt cgcttatctg cccgtgtgta
    28261 atttatgaaa atgattgaag ttgttgccgc catcattgaa cgtgatggca aaattttact
    28321 cgcgcaacgc cccgcccaga gcgatcaggc gggattatgg gagtttgccg gtggtaaagt
    28381 cgagccggat gaaagccagc ggcaggcgct ggtgcgtgag ttacgcgaag aactgggcat
    28441 cgaagcaact gtgggtgagt atgttaccag ccatcagcga gaagtttcgg gccggattat
    28501 ccatcttcat gcctggcacg tacccgactt ccacgggacg ttacaggcac atgaacatca
    28561 ggcgctggtc tggtgctcac ctgaagaggc gctgcaatat ccgctggccc ctgctgacat
    28621 tccattatta gaggcgttta tggctttacg cgccgccaga ccagcggatt agtgctaagg
    28681 gttttgtcat cccgctggca ttgcagcagt attccctcgg ctttaattac cgcgccttca
    28741 gaataatttt tatcctgata aacgcagcac tgagtacagg gctgcgctga ctgcccgcct
    28801 gaactgaaca cttctggcgg tacgtttacc tccacgtccg gacgataatg cgggttagcc
    28861 agtgcaatta atggaaatgc taatactacg gcgaacaatg ctcgactcac agggaactcc
    28921 ttaacgttat tgtctctgct actgataacg gtagccgggt ggcaaaactt tagcgtctga
    28981 gttatcgcat ttggttatga gattactctc gttattaatt tgctttcctg ggtcattttt
    29041 ttcttgctta ccgtcacatt cttgatggta tagtcgaaaa ctgcaaaagc acatcacata
    29101 aacaacataa gcacaatcgt attaatatat aagggtttta tatctatgga tcagacatat
    29161 tctctggagt cattcctcaa ccatgtccaa aagcgcgacc cgaatcaaac cgagttcgcg
    29221 caagccgttc gtgaagtaat gaccacactc tggccttttc ttgaacaaaa tccaaaatat
    29281 cgccagatgt cattactgga gcgtctggtt gaaccggagc gcgtgatcca gtttcgcgtg
    29341 gtatgggttg atgatcgcaa tcaggtacag gtcaaccgtg catggcgtgt gcagttcagc
    29401 tctgccatcg gcccgtacaa aggcggtatg cgcttccatc cgtcagttaa cctctccatt
    29461 cttaaattcc tcggcttcga acaaaccttc aaaaatgccc tgactaccct gccgatgggc
    29521 ggtggtaaag gcggcagcga tttcgatccg aaaggaaaaa gcgaaggtga agtgatgcgt
    29581 ttttgccagg cgctgatgac tgaactgtat cgccacctgg gcgcggatac cgacgttccg
    29641 gcaggtgata tcggggttgg tggtcgtgaa gtcggcttta tggcggggat gatgaaaaag
    29701 ctctccaaca ataccgcctg cgtcttcacc ggtaagggcc tttcatttgg cggcagtctt
    29761 attcgcccgg aagctaccgg ctacggtctg gtttatttca cagaagcaat gctaaaacgc
    29821 cacggtatgg gttttgaagg gatgcgcgtt tccgtttctg gctccggcaa cgtcgcccag
    29881 tacgctatcg aaaaagcgat ggaatttggt gctcgtgtga tcactgcgtc agactccagc
    29941 ggcactgtag ttgatgaaag cggattcacg aaagagaaac tggcacgtct tatcgaaatc
    30001 aaagccagcc gcgatggtcg agtggcagat tatgccaaag aatttggtct ggtctatctc
    30061 gaacgccaac agccgtggtc tgtaccggtt gatatcgccc tgccttgcgc cacccagaac
    30121 gaactggatg ttgacgccgc gcatcagctt atcgccaatg gcgttaaagc cgtcgccgaa
    30181 ggggcaaata tgccgaccac catcgaagcg actgaactgt tccagcaggc aggcgtactg
    30241 tttgcaccgg gtaaagcggc taatgctggc ggtgtagcaa cgtcaggcct tgaaatggcg
    30301 caaaacgctg cgcgcctggg ctggaaagcc gagaaagttg acgcacgttt gcatcacatc
    30361 atgctggata tccaccatgc ctgtgttgag catggtggtg aaggtgagca aaccaactac
    30421 gtgcagggcg cgaacattgc cggttttgtg aaggttgccg atgcgatgct ggcgcagggt
    30481 gtgatttaag ttgtaaatgc ctgatggcgc tacgcttatc aggcctacaa atgggcacaa
    30541 ttcattgcag ttacgctcta atgtaggccg ggcaagcgca gcgcccccgg caaaatttca
    30601 ggcgtttatg agtatttaac ggatgatgct ccccacggaa catttcttat gggccaacgg
    30661 catttcttac tgtagtgctc ccaaaactgc ttgtcgtaac gataacacgc ttcaagttca
    30721 gcatccgtta actttctgcg atagcagcag atatgccagt aaagaaaccc cattagacta
    30781 tttttttgat aatcttcttc gctttcgaac aactcgtgcg cctttcgaga agcaagcatt
    30841 atataatgcc aggccagttc ttcttcaatt gtcccgtttt gaaaagctgt gcttgatatc
    30901 gaggtcatcc atgataattc cgccgcccat attagcttcg ccgaggattt accggagcga
    30961 ttattagcgc aatcagagat atagtctgag ggaaaaacag caaatttatt caacaaggcg
    31021 ataacctgct ctggggcttc ctccatgttt gctttaaagg tattggctcc atggtcgcca
    31081 gaaagaaaat actccattaa ggcacaataa ctttcgctat cttcgatacc ccattgatcc
    31141 tctaaagact cgcgtctttt gcttatgata tcgatcgagt caaagggaag cacatgatat
    31201 tggaaggtat ctttgccagg ttcaggcttt cgcggccaga actccagcgt ttcagaccat
    31261 tgcttatgat agaaccgata aggtgcgatc aattgtagcg cctgtaactt ctcgatactg
    31321 agcggctcaa tacctttagc ctgataataa tgcagttgtt ctttttttgc tttaaaaccg
    31381 gcccgaacaa taagccccat cataattaat agataaagaa aagagcatcc cgcggtaatc
    31441 aggcctcttt cattcaaacc gttggatgtt atcgctgcga acacaaacat tacagcgaca
    31501 acacatgtta aataaaaccc ccacttacaa agcagcatgg ccttattttc tttaatcatc
    31561 cgttcaaaat tactattaaa tatttcccag ccattaaaag aatacttctc gctcccagga
    31621 tggttttgta ataaaacttt tttcatcaca ttccctgtta ttacatactt tagaggcgat
    31681 aattttatcc atgcaaaaaa aatatccaac gaattttctt gatccgctgg atatttcaga
    31741 atatgactcg aatagcacga aagattcact cgcttacgct atcgccccgc ttccgacttc
    31801 atctgctggc ggactttttt tcgcactacg tttacgcggt gcagcctttt tcttatcagc
    31861 actgccacca ctgcccggag ccacaatgcc gcgaaactgc cgcaccggcg tacgtttggc
    31921 ttgatcaata agctgatata gcgtccccac cagcggctgc ataaagtcct gatagcgaca
    31981 ctgcttttcg ctgatttgcg tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt
    32041 cgccatttct ggcagcgaat ggaataacgc ttttccggcg tcggtagagt ggatatagcg
    32101 cccttttttg gtcagaaaac cacgcttgaa cagcagttca ataatcccgg cacgggttgc
    32161 ttctgtcccc agaccatcgg tcgcacgaag gatctttttc agatctttgt cctgcacaaa
    32221 gcgcgcgatc ccggtcatcg ccgaaagcag tgttgcatcg gtaaaatggc gcggcggctg
    32281 ggtttgccgc tctaccactt cacctttttc acacagcaac tcatcgcctt tcgccaccac
    32341 aggcaatggc gtaccgtcgt tttcttcatc gcgctctttg ctgcctaaca gcgtgcgcca
    32401 gcctgcttca gcaagaaaac gggctttagc gacaaatttg cctttggcaa tgtccagttc
    32461 gataacacac ttgcggaaca ccgcatccgg gcagaactgc atcaggtatt gacgggcaat
    32521 caggttatag accttcgctt cgttctccgt caggttgatc gcagaactcc gtgcggtcgg
    32581 gatgatggcg tggtgcgcat cgaccttttt gtcatcccaa cagcggttgc gtatatctgg
    32641 atctaccact gactgcggca acagatccgg tgcatgaacg ctgatggcat tcatcaccgc
    32701 gtggcgtccg gcaaaatgtt cttctggcaa atagcgacaa tcggaacgcg gataagtgat
    32761 tagcttgtgc gtttcgtaca gcttctggca gatatcaagc acgttctgcg cgctcagacc
    32821 gaagcgtttt gccgcttcaa tctgtaacgc cgaaagcgaa aacggcagcg gcgcggattc
    32881 tgattcccgt ttatcgttat agctggtgac aatagccggt tgaccactaa tgcggttaac
    32941 cacatgctcc gccagtggac gatgtaacaa gcgcccttct tcatcctggt acggttcaca
    33001 cgcttcgctc ggttgccaga tagcggtaaa ccgctcatcg gcaggtgtca cgatatgcgc
    33061 tttgacttca aagaagtctt tcgccacgaa gttttcaatc tcttcatcgc ggcgcaccac
    33121 cagcccgagc acgggtgtct gcacgcgtcc cacggaaagt acgccctgat aaccggcatt
    33181 gcgaccgaga atggtatacg cacgggtcat attgatgccg tacagccagt cggcacgcgc
    33241 tcgcgccagc gcagaaacgc acagcggtac aaactcactg ttggaacgaa gacggtcgat
    33301 cgcccgctca accgcctgcg ggttcaggtc gtttatcaag caacgctgca cctgctggcg
    33361 cttttccggt gccagttgca gatagtccag cacttcatcc accagcaatt gcccttcacg
    33421 atccgggtcc ccggcgtgaa cgatttcgct ggcttcatgc aggaagcgtt tgatgacgtt
    33481 aagttgtttg gtcacggagg gtcggggctg taattgccac ttttccggga caatcggcaa
    33541 atccgcaaga ttccagcgcg catagcggct gtcgtaggcg tctggctgcg cctgctcaag
    33601 caggtgaccg atacaccagg tcaccacctg accattaccg cactcgataa agccatcgcc
    33661 tttacggtgc ggtttgggca ggacatcagc aatggcgcgc gccagactcg gtttttcggc
    33721 aataaacaac cgcattgaat taatggatct caaccatggc acgaccgccg cgcgctggca
    33781 ccagttcgcc aatcgccgtc agttcaatgc caaactcggc ggctgtagtt ttaacctcat
    33841 tttctgcttc cggcatgacc gccagcagca aaccgccaga agtttgcgga tcgcacagca
    33901 gatcgcgcac ttcacgcggc atttcaccca tcagatgacc gtagctggca aagttacgtt
    33961 cggtgccgcc aggcactgcg cccaacttaa tgtactcttc aacccccggc aattttggga
    34021 tcgcgtcata gtcaacgcgc gcctgcacac ctgcgccctg acacatttcg ctcaaatggc
    34081 ccagcagacc aaaaccagta acgtcggtca tcgcttttac gccttcgatg ttggcaaagg
    34141 acgcacctgc gatgttcatc cggcacatca cttccgtcgc cagtccctga tgttctggtt
    34201 tcaacagtga ttttttctca gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga
    34261 acagtttgca tccggcttgt gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc
    34321 ccgttaccgc cagaccaaaa atcggctccg gcgcatcgat ggagtgaccg ccagccagcg
    34381 caatacccgc ctgacggcat gcatagcgtc caccttcggt tacttcgcgg gcaatttctg
    34441 gggaaagttt gttaatcggc cagccgagga tcgcaatcgc cataatcggt ttgcccccca
    34501 tcgcgaagat atcgctgatg gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat
    34561 caacgatcgg cataaagaag tcggtggtac tgataacgct ggtgccattg cccagatcgt
    34621 acaccgccgc atcgtcgcgg gtctcattac ccacaagcaa attcggatca acaaacttcg
    34681 cctgctcact gtgcaggatg gtttccaaca cttttgggga aattttacag ccgcaaccag
    34741 ctccgtggct gtattgggtc aaacgaatcg agttctcgct catggacatc tcctgtcaat
    34801 gcaatccggg tatggtaacc ctcattccgt gaagtgataa gtgagagtgt ctgaattcct
    34861 gcgcctttgc tcacaatcca gacagtttcg cgacaattat cagaaataag ttacaaacgg
    34921 cgtcgggtcc gggacgttaa tcgacgtaga tgctttcaac tgcggcgtgc cgaggtagag
    34981 aaaaccgaca attttatcct gctcacggca accgaatgct tcacgcacta ccggactttc
    35041 agttaatgcg ccactgcgcc agatgccgcc aaatccttgg gcaacggctg ccatttgcat
    35101 cgccatgacc gcgcatccgg cagacatttc ctgttcccat cgcgggactt tatgattttc
    35161 ttcgcatttc gccaccaccg tgatgatgag cggtgcgcgg aacggcgcat tacgggcttt
    35221 gtcgatagct ttgtcatcac taccggcagc aatcgccccc tgttccagta cggcgctgaa
    35281 acgctcgcgc ccttcccctt caatcacaaa aaaatgccac ggttgcatgg acttatggtc
    35341 cggcgcacgc atacccgcac gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc
    35401 agccaggcgg gaggcgctac ggcgattgat caatagttcg agtgcatcca tttgattaac
    35461 tcctgtcgtg atatttattc acaaaattaa cacgagagtg gattttgtta cagcacagtc
    35521 cgcaattcct gctgacaagt gccggttggg tcattacgat aaccacatct attgcgcctg
    35581 tgacaggtgt gaccttaagt tgggagaata catgcgaacc ctttggcgat ttattgccgg
    35641 attttttaaa tggacgtggc gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt
    35701 ctttattttc ctcgtactgg ttggtgtggg gatctggatg caggtcagtg gtggtgattc
    35761 gaaagaaacg gccagtcgtg gcgcactgct gctggacatt tctggtgtga tcgtcgataa
    35821 acccgacagt tctcagcggt ttagtaaatt aagccgccag ctgcttggtg ccagttccga
    35881 tcgtctgcag gaaaactcac tgtttgatat cgtcaacacc attcgccagg cgaaggacga
    35941 ccgcaatatc accggtatcg tgatggatct gaaaaacttc gcaggcggcg accagccgtc
    36001 tatgcagtac atcggcaaag ccctgaaaga gtttcgtgac agcgggaaac cggtttatgc
    36061 cgttggcgag aactacagcc aggggcaata ttatctcgcc agtttcgcca ataaaatttg
    36121 gctgtcaccg caaggcgtgg ttgatctgca cggttttgct accaacggtc tgtactacaa
    36181 atcgttgctg gataagctga aagtttccac ccatgtgttc cgcgtgggta cgtataaatc
    36241 tgccgttgaa ccgtttattc gtgatgatat gtcaccggca gcccgcgaag ctgacagccg
    36301 ctggattggt gagctgtggc aaaactatct gaatactgtt gccgctaacc ggcagatccc
    36361 tgctcagcag gtattccctg gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg
    36421 cgataccgcg aaatatgcac tggaaaacaa gctggtcgat gcactggcat cgagtgccga
    36481 aatcgaaaaa gctctgacca aagagttcgg ctggagtaag actgataaaa attatcgcgc
    36541 catcagttat tacgattacg cattgaaaac gccggcagat accggtgaca gcatcggtgt
    36601 cgtcttcgct aatggcgcaa ttatggatgg cgaggaaact caggggaatg ttggcggtga
    36661 taccactgcg gcacaaatcc gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct
    36721 gcgtgttaat agcccaggcg gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc
    36781 agcagcccgg gcagcgggta agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg
    36841 tggttactgg atttccacgc cagctaatta cattgtggct aaccccagca ccctgaccgg
    36901 ttctatcggt atcttcggcg tgatcaccac cgtagaaaat agtctggatt cgattggtgt
    36961 tcatactgat ggtgtctcaa cttcaccgct ggcggatgtt tctatcacca gggcactgcc
    37021 gccggaagcg cagcagatga tgcaattaag cattgagaat ggctataaac gctttatcac
    37081 gctggttgct gatgcgcgtc attcgacgcc ggagcaaatt gataaaatcg cccagggcca
    37141 cgtctggacc ggtcaggatg caaaagctaa cgggctggtc gatagtctcg gggatttcga
    37201 tgatgcggtt gccaaagcag cagagctggc aaaagtgaaa cagtggcatc tggaatacta
    37261 cgttgatgaa ccgaccttct tcgacaaagt gatggacaac atgtctggtt ctgtccgggc
    37321 aatgttgcca gatgcgttcc aggccatgtt acctgcaccg cttgcctcgg tagcctctac
    37381 cgttaaaagt gaaagcgaca agctggccgc gtttaacgac ccacaaaacc gttatgcgtt
    37441 ttgcctgacc tgcgccaacg tgcgttaagt cttgtactga gtggccgaca gatcgtcggc
    37501 cacattattt tttacctcga cgaatcctct tcccgctgtc tcgacccata tttccttatc
    37561 cgcatagtat caggtgcgct cctcctgcct cacgtatata cttttgctct ttcgatatca
    37621 ttcatatcaa tatcatgcaa aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg
    37681 ggatgcagcg ttccgagcag ggttatattc cggtgtcagg tcatctacaa cgccaactgg
    37741 cgctgatgcc ggaattccat cgcccggaga tgccagattt caccattcat gaatatacgc
    37801 cgttgatgga ttcttctgat atgacgccgg aagactggca gcatattgct gaagatatta
    37861 aagcgcacta tgacgactat gatggttttg tcattctaca cggtaccgac acgatggcgt
    37921 ataccgcctc tgcgctgtcg ttcatgctcg agaatctcgg taaaccggtc attgtgacag
    37981 ggtcacaaat cccgctggct gagttacgct ctgacggaca aattaatctg ctgaatgcgt
    38041 tgtacgttgc ggcgaattat ccgatcaacg aagtaacgct ctttttcaat aaccgattgt
    38101 atcgcggcaa ccgcactacc aaagcccatg ccgatggttt tgatgcgttt gcctctccaa
    38161 accttcctcc gttactggaa gctggtatcc atattcgtcg tttgaatacg ccacccgccc
    38221 cgcacggtga aggggaatta atcgttcatc caatcacgcc acaaccaatt ggcgtagtga
    38281 cgatttatcc agggatttct gctgacgtcg tgcgcaattt tctgcgccaa ccggtgaaag
    38341 cattgattct gcgctcctat ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg
    38401 aattacaaga agccagcgat cgcggtattg tggtggtcaa cctgacacaa tgtatgtccg
    38461 gtaaagtgaa tatgggtggt tatgccaccg gtaacgccct cgcccatgcc ggcgttattg
    38521 gcggtgcaga tatgactgta gaagccacgc taaccaaact gcattacctg ctgagccagg
    38581 aactggatac tgaaaccatt cgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc
    38641 cggatgatta aggagactgc aatgccccat cgcgccctgt tactggtcga tttacaaaat
    38701 gatttctgtg ctggtggcgc gctcgccgtg ccggaaggtg acagtacggt agatgtcgct
    38761 aaccgcctga ttgactggtg ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg
    38821 cacccggcga atcacggcag ttttgccagt cagcacggtg tagagcctta tacgccaggc
    38881 caactcgacg gtttgccaca aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc
    38941 gcacaattac atccgttact gaaccaaaaa gcgatcgcag cggtgttcca taaaggcgaa
    39001 aatcctttag ttgacagtta cagtgccttt tttgataacg gccgtcggca gaaaacctct
    39061 ctcgatgact ggttacgcga tcatgaaatc gatgaattga tcgttatggg cctggctact
    39121 gactattgcg tgaagtttac cgtgctggac gcgttacagt taggttataa ggtaaacgtg
    39181 attaccgatg gttgtcgtgg cgtgaatatc cagccccagg acagtgcgca cgcgtttatg
    39241 gagatgtcag cagctggggc aacgctatat acgctggcag actgggaaga gacgcagggg
    39301 taatattagt tgaattcatc tctggcctac atttcctcaa tgacattgta ggccagataa
    39361 tacttgtcag tgttgcattt aacctacttg cagctaaaac acgacgtcct aacaagtata
    39421 tttgccctcc cttaataaca ggcttaaata ctagataact cattcactta aacatagcaa
    39481 taggctttgc tgagatacca aaatcttctt ttaactgctg tttgctcttc atgaccatct
    39541 gaccatttgt gtcaattgtc atatgctgtg cttcggtatt atggcgggct tgccacagca
    39601 tcaccagttg caggcaattc tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt
    39661 tccccagttc aacggcggtc gacaaacgct ggtatacctc aggcgtcatg ctgttgataa
    39721 tgtcatcaag attcataatt tttccgctcc tgtggaataa gttgctgaat cgttttttca
    39781 accgttgatt tcttcgccgt tttcgccatc agtgaagcgt aaagaagcag agttaacaca
    39841 ataacgttcg cctgttggtt gcggcccgtc gggaaagaca tgccccagat gggcatcaca
    39901 gttaccgcaa cgaatttcta tgcgctgcat tccatgtgac aagtctttga tataacgaat
    39961 ggattcttca ctcaccggtt cgtagaaact gggccagcca cagccggaat catacttggt
    40021 ttcggaatga aacagcgggg catcgcagat caaacagtga tatacgccgt cacgcttgtt
    40081 atgcagtaaa cgacccgtaa atggcggttc tgtcccatga ttctgcgtca cgtaaaactg
    40141 catctcggac aaattttttt tcagttcttc tgccgaaggt ttattagcca tttgctcaca
    40201 tctcacttta atcgtgctca cattacgtga ctgattctaa caaaacatta acaccaactg
    40261 gcaaaatttt gtcctaaact tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa
    40321 tcacattttt atcgtaattg ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag
    40381 cccaaaggaa gagtgaggcg agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg
    40441 caatgattga cacgattccg cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc
    40501 ttttattcac taacaaatag ctggtggaat atatgactat caaagtaggt atcaacggtt
    40561 ttggccgtat cggtcgcatt gttttccgtg ctgctcagaa acgttctgac atcgagatcg
    40621 ttgcaatcaa cgacctgtta gacgctgatt acatggcata catgctgaaa tatgactcca
    40681 ctcacggccg tttcgacggt accgttgaag tgaaagacgg tcatctgatc gttaacggta
    40741 aaaaaatccg tgttaccgct gaacgtgatc cggctaacct gaaatgggac gaagttggtg
    40801 ttgacgttgt cgctgaagca actggtctgt tcctgactga cgaaactgct cgtaaacaca
    40861 tcaccgctgg tgcgaagaaa gtggttatga ctggtccgtc taaagacaac actccgatgt
    40921 tcgttaaagg cgctaacttc gacaaatatg ctggccagga catcgtttcc aacgcttcct
    40981 gcaccaccaa ctgcctggct ccgctggcta aagttatcaa cgataacttc ggcatcatcg
    41041 aaggtctgat gaccaccgtt cacgctacta ccgctactca gaaaaccgtt gatggcccgt
    41101 ctcacaaaga ctggcgcggc ggccgcggcg cttcccagaa catcatcccg tcctctaccg
    41161 gtgctgctaa agctgtaggt aaagtactgc cagaactgaa tggcaaactg actggtatgg
    41221 cgttccgcgt tccgaccccg aacgtatccg tagttgacct gaccgttcgt ctggaaaaag
    41281 ctgcaactta cgagcagatc aaagctgccg ttaaagctgc tgctgaaggc gaaatgaaag
    41341 gcgttctggg ctacaccgaa gatgacgtag tatctaccga tttcaacggc gaagtttgca
    41401 cttccgtgtt cgatgctaaa gctggtatcg ctctgaacga caacttcgtg aaactggtat
    41461 cttggtacga caacgaaacc ggttactcca acaaagttct ggacctgatc gctcacatct
    41521 ccaaataagt tgagatgaca ctgtgatcta aaaagagcga cttcggtcgc tctttttttt
    41581 acctgataaa atgaagttaa aggactgcgt catgattaag aaaatttttg cccttccggt
    41641 catcgaacaa atctcccctg tcctctcccg tcgtaaactg gatgaactgg acctcattgt
    41701 ggtcgatcat ccccaggtaa aagcctcttt tgcattacag ggcgcacacc ttctctcgtg
    41761 gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg
    41821 cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg tttggtccgg cggcacaaca
    41881 aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga
    41941 agatgctgat ggcgtagcgc tgacttttga attgacgcaa agcgaagaga cgaaaaaatt
    42001 ctggccgcac gactttacgc tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga
    42061 tcttgaatca catggcgaat ttgaaaccac ctctgccctg catacctact ttaacgtggg
    42121 tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc ttcattgata aagtaaatga
    42181 cgcgaaagaa gatgtactga ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt
    42241 gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc
    42301 cgtaggccac cagcatcatc tgaacgttgt cggctggaac ccgggaccgg cgctttcagt
    42361 tagcatgggc gacatgccgg atgatggcta caaaacattt gtttgtgtag aaacggctta
    42421 cgcttcagaa acgcaaaaag tgaccaaaga gaaacctgca catctggcgc aatccattcg
    42481 cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta
    42541 tcaacatcac accatatcca gcgcagtttt tccttttggt gccggatacg ctttatccag
    42601 catggttaat tccgctgacg aaagttcgac ctcaagcaca gccgcattct gttggacatg
    42661 ggcaatcgtg gccgcttttg gaatcgccat cacaccctga tggctgatta cccacgccaa
    42721 caatacttgt gccgcgctga tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt
    42781 taacagtcca ttgcgcaacc gcccggcctg ggctaatgga ctgtaagcca tcaccggcat
    42841 ctgctgttgc tggcaccagg ggagtagatc gtactcaatc cctcgtgaac cgagatggta
    42901 aagcacctga ttagtggcac actgatttcc ccccggcagc tgccagagtt cctgcatatc
    42961 agcatgatca aggttagaaa cgccccagcg gcggattttt ccctgggcga tcaatttttc
    43021 catcgctgcc acagtctctt caaaagcgaa actgccagac cagtgtaata agtagagatc
    43081 gagataatca gtattgagac ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc
    43141 gccagcattc cacggataga ctttagagac gagaaagacg ttatcgcgca gaccggttaa
    43201 tgcttcccca accacctttt cggcaccgcc atcggcatac atttcggcgg tatcaatgag
    43261 ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact tctgttttgc gctgactggc
    43321 atcttcgccc atataccatg ttccctgccc tatggctggc agtgagacat cgccactaaa
    43381 ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg caccaccgta atgcaaaaca
    43441 gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa agatgcatta tcagaatttg
    43501 taggtgatcc cggtagaaat caggccagtc caggatttat ccaccatcgg gctgtcagta
    43561 acttcatcag acagacgggt atagcgcgcg gtaccgtaaa cactccagtc gccgaggaag
    43621 ttgtagctgg cgctcagctc caggtaaggg ctccagctgt cgttcgggtt atagccacgc
    43681 agaccgctgc gagcggactc tttgcgcgat acgccataat agtattcgtt ctggttttcg
    43741 ctgttccact gcacaccgat acccggggtc acggtcaggc cgccgttggt gtaacgatac
    43801 aaccaggcca tatcccagac gatgccgttg ctgttatcca gggtatcgcc agccagggtg
    43861 gtacgcaggt aaccgtactg ggtaaagtga gcataagaca gaccagccat catggtgctc
    43921 ttacggtcat ccaggtgacg catttggtga tcgccactgt ctttcgcttt gaagtaaagc
    43981 ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt cattccacag gtagtaacca
    44041 ccacctaagc cacggaacca gaagttatcg ccttcatagt tgattaccgg tactgggtaa
    44101 acatcggtat cgtaatcttt atatgggtgc tcaacgacac ctacgcctgc gcccagggaa
    44161 aatttacctt cagcgtgcgc tacgcctgca gacgttgcga taagcactcc aagtgccaga
    44221 agtttgagtt tggtcacaat taatcattcc ttaaacaaat gtttagcggg cgacaaagtt
    44281 tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc attttttaaa gtaactgttt
    44341 aattttcctg acgcggatga caccgcgctt acagccaaat gaatttagcg ttactggcta
    44401 ccctggtctt tacattaatt atgcaaaatt tatggatgag ttgttgatat gccattgaaa
    44461 ttaagaaagc cgtgcaggca agttttccat ttgccatcta cgcttaattt tgaaggtgta
    44521 tcaccgggca cgttgttctc atcgtcgata aaatggcatg agagttgctg tgttttagca
    44581 agagacgtcg ttcagtttac ctcttccggg agcctctact attcatatga acggctctta
    44641 acctgtgcta aaaaacgaaa ggacggcata ccatgaatat attcgatcac tatcgccagc
    44701 gatatgaagc tgccaaggac gaagagttca cactgcagga gtttcttacc acttgtcggc
    44761 aagatcgcag tgcttatgcc aacgcggctg agcggctatt gatggctatc ggtgagcctg
    44821 tcatggtcga tacagcccag gaacccagac tttctcgact cttttctaac cgggtcattg
    44881 cacgttatcc ggcatttgaa gagttttacg gcatggaaga cgcgattgaa cagattgtct
    44941 cttatctgaa acacgcggct caggggctgg aagagaagaa acaaatcctg tatctgctgg
    45001 ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact gaaatcatta atgcagctcg
    45061 taccgattta tgtattgagc gccaacggtg agcgtagccc ggtcaacgat catccgttct
    45121 gtcttttcaa tccgcaggaa gatgctcaga ttctggaaaa agagtatggc attcctcgcc
    45181 gttatctcgg caccatcatg tcgccatggg cggcaaaacg cctgcatgaa tttggtggcg
    45241 atatcactaa gttccgggta gtgaaggtct ggccgtcaat tctgcaacaa attgctatcg
    45301 ccaaaacgga acccggtgat gagaacaacc aggacatctc cgcgctggtt gggaaagtcg
    45361 atattcgtaa actcgaacac tacgcgcaga atgacccgga cgcctacggc tattccggtg
    45421 cgctgtgccg cgccaaccaa gggatcatgg aatttgtcga gatgtttaaa gcaccgataa
    45481 aagtgctgca tccgctgtta accgccactc aggaaggtaa ctacaacggg acggaaggta
    45541 tctccgccct gccgttcaac gggattattc tcgcccactc gaacgaatcc gaatgggtca
    45601 ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg tgtttacatc gtgaaggtgc
    45661 cgtattgctt acgcatttcc gaagagatca aaatctacga gaaattgctt aatcacagtg
    45721 aattgactca cgccccctgc gcccctggca cgctggaaac gctgtcacgt ttttccattc
    45781 tttcgcgcct taaagagcca gaaaactcca gcatttattc aaagatgcgg gtttatgatg
    45841 gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta tcaggaatat cgtgactacg
    45901 ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt tgcgtttaag atcctctccc
    45961 gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc ggtccatctg ttctacgtcc
    46021 tggaacagca gatcgagcgc gagcagttcc cacaagagca ggcagaacgc tatctggagt
    46081 tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat cggcaaagag atccagacgg
    46141 cctaccttga atcctattcc gaatatgggc aaaacatttt cgaccgttat gttacctacg
    46201 cggatttctg gattcaggat caggagtatc gcgatccgga taccgggcag ctgtttgacc
    46261 gcgagtctct taacgccgag ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa
    46321 aagatttccg caacgagatt gttaacttcg tactgcgcgc cagagcgaat aacagcggac
    46381 gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct
    46441 ccaataccga ggagctgttg ccggttatct cgtttaacgc caaaacgtca accgacgagc
    46501 agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg
    46561 tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg
    46621 gtacacatgt taccgggcct acaacgacag cgaaccgtgg gcctgagaag cggcaacaca
    46681 ggcgtagcat acagttggca aatgtagtac ggggggcata tgacctggtt tattgaccgg
    46741 cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc gttttttacg ccgttataaa
    46801 gcgcaaatta aacagtcgat ctccgaggcc attaataagc gttcggtgac tgacgtcgac
    46861 agcggcgaat ccgtatccat tcccacggaa gatattagcg aaccgatgtt tcatcagggg
    46921 cgtggcggtc tgcgccaccg cgtgcatccg ggcaatgacc atttcgtcca gaacgaccga
    46981 attgaacgtc cccagggtgg cggcggaggt tccggtagtg gtcagggcca ggccagccag
    47041 gatggtgaag gtcaggatga atttgtcttt cagatttcga aagatgagta tcttgatttg
    47101 ctctttgaag atttggcctt accgaatctg aaacaaaacc aacaacgcca gctgaccgaa
    47161 tataaaacgc atcgggcggg ttataccgct aacggcgttc cggcaaatat cagcgttgtg
    47221 cgctccttgc agaactcact ggcgcgacgc acagccatga cggcaggcaa gcggcgggaa
    47281 cttcatgcac tggaagagaa tttggccatc atcagcaaca gtgaacctgc gcagctgctg
    47341 gaagaggaac gtctgcgcaa agaaattgca gaattacgtg ccaaaattga acgcgtccct
    47401 tttattgaca ccttcgattt acgttacaag aactacgaga agcggcccga tccctccagc
    47461 caggcagtga tgttttgcct gatggacgtt tccggttcaa tggatcaatc cactaaagat
    47521 atggctaagc gtttttatat tctgctgtat ctgttcctca gcagaacgta taagaacgtg
    47581 gaagtcgtat acatccgcca tcatacccag gcgaaagaag tcgatgaaca tgagtttttc
    47641 tactcgcagg aaacaggcgg caccattgtt tccagcgccc tgaaactgat ggatgaggta
    47701 gtgaaagagc gttataaccc ggcacagtgg aatatttacg ctgcacaagc atcggacggc
    47761 gataactggg ccgatgactc tccgctttgc catgaaatcc tggcgaaaaa attattacct
    47821 gttgttcgtt attacagcta tatcgaaatt acccgtcgtg cacatcagac attgtggcga
    47881 gaatatgagc atctgcaatc tactttcgac aactttgcga tgcagcacat ccgcgaccag
    47941 gatgatattt atccggtgtt ccgtgaactg tttcataaac aaaatgcaac agctaaagac
    48001 taaaactatc agccaggtca ttatcgcctg gctgattttt agcttactgt aaattatctc
    48061 atttattaca tacattagct tacaatcgct ttaaatatga cagcgtaacc tttacataat
    48121 ttagttccag aaaacaatca ttcggaaaaa tgattcagtc aacacgtatt tccatggggt
    48181 tattctttaa atattgttta tcgttaacga aaattgatcc tggtcaaaac tatatatctc
    48241 tgccatcaat aaaatccagc actcacattg ctctcctttt tatggtttct atgggtacac
    48301 aaaaattaaa agctcaaagc ttttttattt tcagtttatt gctgacgtta attttatttt
    48361 gcatcaccac attatataac gaaaacacaa atgtaaaact catcccacag atgaattacc
    48421 tgatggttgt tgtggctttg tttttcctta acgccgtcat ttttcttttc atgttaatga
    48481 aatatttcac taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg
    48541 gccttatcta tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac
    48601 tgatccagac aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta
    48661 tttgtttaac ctcgctggca ctcttttgtt atggaaaaga caacatcctt gacaacaata
    48721 agaaaaaaac gggaatcctg ttgctggcgc tgattccttt tttagttttt ccccttctgg
    48781 cacacaatct gagcagttat aacgctgact attctttgta tgtcgtcgac tactgtccgg
    48841 acaaccatac tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat
    48901 ttttactgtt ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag
    48961 cattattatg tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata
    49021 attacaccat ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt
    49081 cttttctgat ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg
    49141 atgtgctgac caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt
    49201 cgcgacctgt tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca
    49261 tcaatgccca atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta
    49321 tccagcaaag catccgccct gatgatattt tagcgcgact ggagggtgag gtgtttggct
    49381 tgctatttac cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa
    49441 atgtcgaact cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca
    49501 gtattggcac ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag
    49561 cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata
    49621 tttaagtgca aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta
    49681 ctattacccc taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg
    49741 tgccaggccg caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat
    49801 ttatcatgaa attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc
    49861 tgacatcttc cttccttatt tttgaacttg tcgccagcga cagagcaatg agtgcctatc
    49921 tgcgctatat cgtgcagaaa gcagactcct cctttcttta tgataagtat cagaatcaga
    49981 gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac
    50041 agcggcgcgc catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga
    50101 ctgcccataa atacccgggc ttacgcggca cactacaaac cgcatccact gactgcgaca
    50161 caattgtgga agctgcagca ctattacccg cttttgatca ggcagtggaa ggcaaccgcc
    50221 accaggatga ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg
    50281 atctcaatga ccgctatgtc tatttttatg agccggttaa tgttgaatac tttgcgatga
    50341 ataactggtc cttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg
    50401 tatttaccgg gcgtaccgta ttgtcgagca tttaccagga tcagcgtact aaacagaacg
    50461 tgatgagttt gctgacgccg gtatatgtcg cagggcagct aaaagggatt gtgctgctgg
    50521 atattaacaa aaacaatttg cggaatatct tttacactca tgaccgccct ctcctctggc
    50581 gttttctcaa tgtcacgcta accgataccg attcggggcg cgacattatc atcaaccaga
    50641 gcgaagataa tctgttccag tacgtcagtt acgtccatga cttaccgggc ggcattcgtg
    50701 tctcgttatc cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct
    50761 ggattttgac ggcgttaatt ttgctgaata tggtccggat gcacttccgt ttataccaaa
    50821 atgtgtcgcg agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa
    50881 cccctgaact ggagcagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta
    50941 ttgcaattga catggacaag ttaaagcaaa taaatgacac cctcggtcat caggaggggg
    51001 atttagcgat tacgttatta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg
    51061 ccatccgact cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg
    51121 cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaaa
    51181 tcggcttctc ttccggtatt tacgcgatga aagaaaacga tacgttacat gatgcgtata
    51241 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
    51301 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
    51361 gcgtgacgca tcagcggtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
    51421 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctctag
    51481 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
    51541 tggcgatcct cgctgccgat caacaggccg atctgagcca actcgccagc catataggcg
    51601 gattacgcgc ttcgttggcc agcccggcgg aagtcgatga actcacaggc tgtgtcttcg
    51661 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
    51721 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
    51781 aaaccacgga ttatttgcgc attgcgcaac cagaactgct caatttccgc cgcactgcgt
    51841 aactagcctg tccgttcaat aagcagaacg gacaaaacca gcacaacagc aatggcaaaa
    51901 aacgatgacg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
    51961 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
    52021 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
    52081 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
    52141 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
    52201 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
    52261 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
    52321 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
    52381 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacctt aatgggcagc
    52441 cagccgcaac tggtcgccgg gctgctggta ggcactgttt taggcgtggc gctgtttcgc
    52501 ggcgtaccgg tcggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
    52561 tagttaatcc cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa
    52621 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
    52681 tcactaatcc ctgaagtgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
    52741 accccgtctc ttttactatc agtcgcgcaa gattgcgttc actcatcgca aaaaagccag
    52801 cccattgccc taatgacccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
    52861 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
    52921 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
    52981 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
    53041 ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
    53101 tcacttgatt gctatgttcc accccgcccg gtatccaaac cgcatattgc ggcggcacca
    53161 tccacaaagc attttccacc gtacaggtaa ttgcaccatg taatgctagt atcagctgtc
    53221 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
    53281 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca
    53341 ttttgtccga atttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
    53401 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
    53461 cggcaaaaac aggattgtcc ttatcgctgg cattctgatg attgccacta cattacgcgt
    53521 caccttcacc ggcgcagcac cgttactcga tacgattcgt tccgcttact cgctgacgac
    53581 agcgcaaacc ggcttattaa ccaccctgcc attattggcc tttgcgctaa tctcaccttt
    53641 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
    53701 gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct tacttattat ttggcggtac
    53761 agcggtcatt ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg
    53821 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
    53881 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
    53941 cgcgttgttc atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg
    54001 gcgaagccaa caacatgcaa atttgagtac ctcgcgcgcc ttacatactc ggggtatctg
    54061 gcgttcgccg cttgcctggc aggtcacatt gtttcttggg atcaactcac tggtctatta
    54121 cgtgattatt ggctggcttc cggcgatcct catcagtcgc ggctatagcg aagcacaggc
    54181 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatccctct
    54241 tttcttacat catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc
    54301 agtgggcgct gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
    54361 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
    54421 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
    54481 agcctgtggg ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc
    54541 actgatgggt gttgccatac tttcactact gatggcgatt ttcggacttt gcgccgggag
    54601 agacaaagaa attcgctaat atccggtgct atagtgacgt gacaaatcat gcgtgaaagg
    54661 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga
    54721 tggttatcgc gtactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc
    54781 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
    54841 cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt gcagaactgg cgcaacacga
    54901 gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctctacgc
    54961 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
    55021 attttagtac agcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg
    55081 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg
    55141 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
    55201 taccaatctg cggcggttta ggtgcttctg gctgagaaga ggttgaacaa ccagccagga
    55261 ccagcaagca aggcaaagca aaactgataa ttttcattta ttgatctcac acatttatct
    55321 acgcttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
    55381 aaacatatta cgtcttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
    55441 aataatcagc atttacccgc cgtatcctgg agttgtttcg tgtcagatca gattatcgcc
    55501 cgcgtctcgc aatcccttgc caaagaacag tcactagaaa gtctggtccg acagcttctg
    55561 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
    55621 gcgcgcctgc agcatattat gtttgcccgt aacagccaga aaatgcacat cccggagaat
    55681 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcta ttgatgaaaa ctgctttttc
    55741 agcgatgaag tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggaatcacc
    55801 acatttctga gtacaccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
    55861 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc
    55921 ggactgattg cacaatatat tcaaaaagag gcgttggttg aacagctgcg cgaagccaat
    55981 gccgcgctga ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg
    56041 atttttgaaa atctgacgac gctgttttct ctcgcccggc atcttaacca taagataatg
    56101 atcgcgttta tcgatctgga taacttcaaa ttaatcaatg atcgttttgg tcataatagt
    56161 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
    56221 gttattggtc gtctcggcgg tgatgagttt ttggttgttt cactaaataa cgagaatgcg
    56281 gatatttcgt cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt
    56341 gatgttgatt tgtattatcc tggtgccagt cttggcattg tagaagtcga tcctgaaacg
    56401 accgatgcag acagtgccct gcatgctgcc gatatcgcga tgtatcagga gaaaaaacac
    56461 aaacagaaaa caccttttgt cgcgcattca gcgccacatt cctgaggcgt attcacatcc
    56521 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc tttattacag
    56581 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
    56641 gaaaaaaaca acgattatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct
    56701 gggatggtgg taacgtcacc tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
    56761 caatttaagc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
    56821 acggcaacca cgaagctgcc aaaattgaag ccatcgactt taccaaagcc aaacagcgtg
    56881 ctgatccatc cgccaactac ggcaccgact atccccagca ggatagtcat aaagaatcca
    56941 ccaccatctt tacctggcat gatccacttc gccagaatac cggcaataag cccaaaaata
    57001 atccatgaca gaatgcccat tgtttcctca cttatctgtt ttgcattagc gggttagtcg
    57061 ctgataaaaa gcatagcaca acatcgggag ggcaagattt gtgacgagca tcacggagga
    57121 tttttttgcg gatggcacag aaattgcgcc atcaacgatc agtgataatt accaaccaca
    57181 aacatcatgt tcgttttccg tgtcttgaca tcctccacgc cctgaatgac gaggatccct
    57241 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gggcggcctg
    57301 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcgc
    57361 cgtttacatc ggcgttcgct gtatatccac atacctggca tctgaattga ctttgtgaca
    57421 ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg
    57481 gaacagcaag cacctgaccg ccac
//
LOCUS       DAZFKM010000016        55197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFKM010000016 DAZFKM010000000
VERSION     DAZFKM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..402
                     /locus_tag="ACHKAY_001066"
     CDS             <1..402
                     /locus_tag="ACHKAY_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO5699781.1"
                     /translation="IIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDS
                     FNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFF
                     RIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            399..891
                     /locus_tag="ACHKAY_001067"
                     /pseudo
     CDS             399..891
                     /locus_tag="ACHKAY_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
     gene            complement(933..1883)
                     /gene="corA"
                     /locus_tag="ACHKAY_001068"
     CDS             complement(933..1883)
                     /gene="corA"
                     /locus_tag="ACHKAY_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO5699782.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2035..2091)
                     /gene="ysgD"
                     /locus_tag="ACHKAY_001069"
     CDS             complement(2035..2091)
                     /gene="ysgD"
                     /locus_tag="ACHKAY_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO5699783.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2253..3017
                     /gene="yigE"
                     /locus_tag="ACHKAY_001070"
     CDS             2253..3017
                     /gene="yigE"
                     /locus_tag="ACHKAY_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO5699784.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3164..5326)
                     /gene="uvrD"
                     /locus_tag="ACHKAY_001071"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3164..5326)
                     /gene="uvrD"
                     /locus_tag="ACHKAY_001071"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO5699785.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5410..6126)
                     /gene="yigB"
                     /locus_tag="ACHKAY_001072"
     CDS             complement(5410..6126)
                     /gene="yigB"
                     /locus_tag="ACHKAY_001072"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO5699786.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6126..7022)
                     /gene="xerC"
                     /locus_tag="ACHKAY_001073"
     CDS             complement(6126..7022)
                     /gene="xerC"
                     /locus_tag="ACHKAY_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO5699787.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(7019..7726)
                     /gene="yigA"
                     /locus_tag="ACHKAY_001074"
     CDS             complement(7019..7726)
                     /gene="yigA"
                     /locus_tag="ACHKAY_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO5699788.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7723..8547)
                     /gene="dapF"
                     /locus_tag="ACHKAY_001075"
     CDS             complement(7723..8547)
                     /gene="dapF"
                     /locus_tag="ACHKAY_001075"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO5699789.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8584..8787)
                     /gene="yifL"
                     /locus_tag="ACHKAY_001076"
     CDS             complement(8584..8787)
                     /gene="yifL"
                     /locus_tag="ACHKAY_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5699790.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9137..9691
                     /locus_tag="ACHKAY_001077"
     CDS             9137..9691
                     /locus_tag="ACHKAY_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO5699791.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            10029..11537
                     /locus_tag="ACHKAY_001078"
     CDS             10029..11537
                     /locus_tag="ACHKAY_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5699792.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11534..12859
                     /locus_tag="ACHKAY_001079"
     CDS             11534..12859
                     /locus_tag="ACHKAY_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO5699793.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            12907..13227
                     /gene="cyaY"
                     /locus_tag="ACHKAY_001080"
     CDS             12907..13227
                     /gene="cyaY"
                     /locus_tag="ACHKAY_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO5699794.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13267..15813)
                     /gene="cyaA"
                     /locus_tag="ACHKAY_001081"
     CDS             complement(13267..15813)
                     /gene="cyaA"
                     /locus_tag="ACHKAY_001081"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO5699795.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            16200..17141
                     /gene="hemC"
                     /locus_tag="ACHKAY_001082"
     CDS             16200..17141
                     /gene="hemC"
                     /locus_tag="ACHKAY_001082"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO5699796.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            17138..17878
                     /gene="hemD"
                     /locus_tag="ACHKAY_001083"
     CDS             17138..17878
                     /gene="hemD"
                     /locus_tag="ACHKAY_001083"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO5699797.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            17900..19099
                     /gene="hemX"
                     /locus_tag="ACHKAY_001084"
     CDS             17900..19099
                     /gene="hemX"
                     /locus_tag="ACHKAY_001084"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO5699798.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            19102..20298
                     /gene="hemY"
                     /locus_tag="ACHKAY_001085"
     CDS             19102..20298
                     /gene="hemY"
                     /locus_tag="ACHKAY_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO5699799.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            20977..21114
                     /locus_tag="ACHKAY_001086"
                     /pseudo
     CDS             20977..21114
                     /locus_tag="ACHKAY_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(21222..22457)
                     /gene="aslB"
                     /locus_tag="ACHKAY_001087"
     CDS             complement(21222..22457)
                     /gene="aslB"
                     /locus_tag="ACHKAY_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5699800.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(22604..22680)
                     /locus_tag="ACHKAY_001088"
     tRNA            complement(22604..22680)
                     /locus_tag="ACHKAY_001088"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22644..22646),aa:Pro,seq:tgg)
     gene            complement(22723..22809)
                     /locus_tag="ACHKAY_001089"
     tRNA            complement(22723..22809)
                     /locus_tag="ACHKAY_001089"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22773..22775),aa:Leu,seq:cag)
     gene            complement(22830..22905)
                     /locus_tag="ACHKAY_001090"
     tRNA            complement(22830..22905)
                     /locus_tag="ACHKAY_001090"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22870..22872),aa:His,seq:gtg)
     gene            complement(22964..23040)
                     /locus_tag="ACHKAY_001091"
     tRNA            complement(22964..23040)
                     /locus_tag="ACHKAY_001091"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23004..23006),aa:Arg,seq:ccg)
     gene            complement(23143..24528)
                     /gene="yifK"
                     /locus_tag="ACHKAY_001092"
     CDS             complement(23143..24528)
                     /gene="yifK"
                     /locus_tag="ACHKAY_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5699801.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24719..25459)
                     /gene="wecG"
                     /locus_tag="ACHKAY_001093"
     CDS             complement(24719..25459)
                     /gene="wecG"
                     /locus_tag="ACHKAY_001093"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO5699802.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25462..26814)
                     /gene="wzyE"
                     /locus_tag="ACHKAY_001094"
     CDS             complement(25462..26814)
                     /gene="wzyE"
                     /locus_tag="ACHKAY_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO5699803.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26811..27890)
                     /gene="rffT"
                     /locus_tag="ACHKAY_001095"
                     /gene_synonym="wecF"
     CDS             complement(26811..27890)
                     /gene="rffT"
                     /locus_tag="ACHKAY_001095"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO5699804.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(27887..29137)
                     /gene="wzxE"
                     /locus_tag="ACHKAY_001096"
     CDS             complement(27887..29137)
                     /gene="wzxE"
                     /locus_tag="ACHKAY_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO5699805.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(29139..30269)
                     /gene="rffA"
                     /locus_tag="ACHKAY_001097"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(29139..30269)
                     /gene="rffA"
                     /locus_tag="ACHKAY_001097"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO5699806.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30274..30948)
                     /gene="rffC"
                     /locus_tag="ACHKAY_001098"
                     /gene_synonym="wecD"
     CDS             complement(30274..30948)
                     /gene="rffC"
                     /locus_tag="ACHKAY_001098"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO5699807.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30926..31807)
                     /gene="rfbA"
                     /locus_tag="ACHKAY_001099"
     CDS             complement(30926..31807)
                     /gene="rfbA"
                     /locus_tag="ACHKAY_001099"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5699808.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31826..32893)
                     /gene="rffG"
                     /locus_tag="ACHKAY_001100"
     CDS             complement(31826..32893)
                     /gene="rffG"
                     /locus_tag="ACHKAY_001100"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5699809.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32893..34155)
                     /gene="wecC"
                     /locus_tag="ACHKAY_001101"
     CDS             complement(32893..34155)
                     /gene="wecC"
                     /locus_tag="ACHKAY_001101"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO5699810.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(34152..35282)
                     /gene="wecB"
                     /locus_tag="ACHKAY_001102"
     CDS             complement(34152..35282)
                     /gene="wecB"
                     /locus_tag="ACHKAY_001102"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5699811.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35338..36384)
                     /gene="wzzE"
                     /locus_tag="ACHKAY_001103"
     CDS             complement(35338..36384)
                     /gene="wzzE"
                     /locus_tag="ACHKAY_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO5699812.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36396..37499)
                     /gene="wecA"
                     /locus_tag="ACHKAY_001104"
     CDS             complement(36396..37499)
                     /gene="wecA"
                     /locus_tag="ACHKAY_001104"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO5699813.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(37739..38998)
                     /gene="rho"
                     /locus_tag="ACHKAY_001105"
     CDS             complement(37739..38998)
                     /gene="rho"
                     /locus_tag="ACHKAY_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO5699814.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(39083..39184)
                     /gene="rhoL"
                     /locus_tag="ACHKAY_001106"
     CDS             complement(39083..39184)
                     /gene="rhoL"
                     /locus_tag="ACHKAY_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO5699815.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39325..39654)
                     /gene="trxA"
                     /locus_tag="ACHKAY_001107"
     CDS             complement(39325..39654)
                     /gene="trxA"
                     /locus_tag="ACHKAY_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO5699816.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39785..41050
                     /gene="rhlB"
                     /locus_tag="ACHKAY_001108"
     CDS             39785..41050
                     /gene="rhlB"
                     /locus_tag="ACHKAY_001108"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO5699817.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            41184..42668
                     /gene="gppA"
                     /locus_tag="ACHKAY_001109"
     CDS             41184..42668
                     /gene="gppA"
                     /locus_tag="ACHKAY_001109"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO5699818.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(42715..44736)
                     /gene="rep"
                     /locus_tag="ACHKAY_001110"
     CDS             complement(42715..44736)
                     /gene="rep"
                     /locus_tag="ACHKAY_001110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO5699819.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            44953..45402
                     /locus_tag="ACHKAY_001111"
     CDS             44953..45402
                     /locus_tag="ACHKAY_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO5699820.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            45601..45882
                     /gene="ppiC"
                     /locus_tag="ACHKAY_001112"
     CDS             45601..45882
                     /gene="ppiC"
                     /locus_tag="ACHKAY_001112"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO5699821.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(45969..47444)
                     /gene="ilvC"
                     /locus_tag="ACHKAY_001113"
     CDS             complement(45969..47444)
                     /gene="ilvC"
                     /locus_tag="ACHKAY_001113"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO5699822.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            47594..48487
                     /gene="ilvY"
                     /locus_tag="ACHKAY_001114"
     CDS             47594..48487
                     /gene="ilvY"
                     /locus_tag="ACHKAY_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO5699823.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48539..50083)
                     /gene="ilvA"
                     /locus_tag="ACHKAY_001115"
     CDS             complement(48539..50083)
                     /gene="ilvA"
                     /locus_tag="ACHKAY_001115"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO5699824.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(50086..51936)
                     /gene="ilvD"
                     /locus_tag="ACHKAY_001116"
     CDS             complement(50086..51936)
                     /gene="ilvD"
                     /locus_tag="ACHKAY_001116"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO5699825.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52001..52930)
                     /gene="ilvE"
                     /locus_tag="ACHKAY_001117"
     CDS             complement(52001..52930)
                     /gene="ilvE"
                     /locus_tag="ACHKAY_001117"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO5699826.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(52950..53213)
                     /gene="ilvM"
                     /locus_tag="ACHKAY_001118"
     CDS             complement(52950..53213)
                     /gene="ilvM"
                     /locus_tag="ACHKAY_001118"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO5699827.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(53210..54856)
                     /gene="ilvG"
                     /locus_tag="ACHKAY_001119"
     CDS             complement(53210..54856)
                     /gene="ilvG"
                     /locus_tag="ACHKAY_001119"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO5699828.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54859..54909)
                     /gene="ilvX"
                     /locus_tag="ACHKAY_001120"
     CDS             complement(54859..54909)
                     /gene="ilvX"
                     /locus_tag="ACHKAY_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO5699829.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(54996..55094)
                     /gene="ilvL"
                     /locus_tag="ACHKAY_001121"
     CDS             complement(54996..55094)
                     /gene="ilvL"
                     /locus_tag="ACHKAY_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO5699830.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
BASE COUNT        13570 a        14823 c        13968 g        12836 t
ORIGIN      
        1 ataatcaaag aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct
       61 cttatcctgc aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac
      121 ttcgaggaca gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata
      181 tctcttttaa gttttcaaaa atacgctata ggatggagtg caacaattca ttttgtagca
      241 caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga
      301 ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc
      361 accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa
      421 ttatattatg tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac
      481 agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc
      541 catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc
      601 aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct
      661 tcactgacta taaaaaaata tatcgaaggg ccgtatgaag atctctactg tttcccgaca
      721 attaagagca atgataattg catcgtgaaa aattacttaa tggttattaa tgaataccct
      781 tatcgtaaca ctcatattgg tattaacgat tttactgagt atcaattaac acaagaaaat
      841 aaaatagagc gagttttcaa tccgcatgac tttaaaaaag acaattttta gaaataagcc
      901 ggataaaaaa atagctttat ccggcactca tattacaacc agttcttccg cttaaagtac
      961 agatacggtg ccaggcccgc gaggatcata aagataatcg cgcctgggta gccgaaactc
     1021 cacttcaact ctggcataaa ttcaaagttc atcccgtagc tggaagccac cagcgtcggt
     1081 ggcaggaaca ccacggatac caccgagaag attttgatga tgcggttctg ctcgatgttg
     1141 ataaagccca tcgccgcctg catcaggaag ttcaccttct ggaacaggga ttcgttatgc
     1201 ggcagcaggg attcgatatc tcgcaggatt tcacgcgcct gctccagttg cccacccggt
     1261 aaacgcgcct tgcgtaccag gaagttgagc gcgcgctggg tatccatcag acacaggcga
     1321 actttccagc cgatatcttc cagttccgcc agcgtggaga gcgcctcgtc gtactcatcg
     1381 ccctgatgcc cttccataat cacccggctc aactgctcca ggtcgctata gatattttca
     1441 atttcatctg ccaactgttc gattttggtt tcgaacagat ccagcagcaa ctcgtaggcg
     1501 ttaccgtcta ccatcgactg gctacgggca cgcatacgat acagacgaaa agcgggcagt
     1561 tcacgctcac gcagagtaaa cagacgacca tcacggatgg taaatgccac agtggagtta
     1621 ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt gaatatgcag gccgtcgtcg
     1681 tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt ccgggcgggt tgccaggctc
     1741 tggccaagtt cagattgtac gcgcagtcgc tcgtcgtcgt ccggttcgac aagatcaatc
     1801 catactgcat ttacaagggg ttgtgactct tcgacttcca gccgggtcag tcggttattt
     1861 tccagttgaa atgcgctcag catgaccggg actcccaatg cttaaaatat cggacagttc
     1921 ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa cagcctgact cagcgcgacg
     1981 ggaaaaattg aggtcgctga caaccgctaa ggctatcagc aaaaagggat agccttagga
     2041 gttgatcctg gatgacagga tagtgagcca gtatctactg ggtgtgtcca aggcgaatgt
     2101 cctcttagag tgatcgtgcg cgcatgttac gccagcaaaa ttttgccgtc aacacgcaac
     2161 agaacaccac agaacaatag ttgaacttcg cgctttttgg tactcgtcaa ggtcgctaac
     2221 aggataaaaa aatccaacat ccaggaccta taatggcgca tcggctactc attggtaaag
     2281 gaatgatcac cttgaatctc aaacggattt ttctcaccct caccttactt cccttgtttg
     2341 ctgttgccgc tgatgattgc gcactctcag atccgacgct gaccgtacag gcgtataccg
     2401 ttaatcctca aacagagcgg gtgaaaatgt actggcaaaa agccaatggc gaagcgtggg
     2461 gaactttaca tgctctgctg gcggatatga atagtcaggg tcaggtgcag atggcgatga
     2521 acggcggcat ctatgatgaa agctatgcgc cgctcggttt gtacatcgaa aacggtcagc
     2581 agaaggtggc gttaaatctc gcttcaggtg aagggaattt ctttatccgt cctggcggcg
     2641 tgttttatgt cgcgggagat aaagtcggca tcgttcgtct ggatgccttc aaaaccagta
     2701 aagagattca gtttgcggtg cagtcagggc caatgttgat ggaaaacagt gtaattaatc
     2761 cgcgtattca tcccaacgtc gcctcacgta aaattcgtaa cggtgttggg attaataaac
     2821 atgggaatgc cgtgtttttg ttgagccagc aggcgacaaa tttttatgat tttgcctgtt
     2881 atgccaaagc gaaactgaac gttgagcagc tgctttatct tgatggcacg atttcacata
     2941 tgtatatgaa aggcggggca atcccgtggc aacgctatcc ttttgtcacc atgatttccg
     3001 tagagcggaa aggttaaaac gatagttttg tgtcagatac tgaagatggc gcagatgccg
     3061 gatgcggcgt aaacgcccta tccggcctac atgacgttgc aatttattga atctgaatga
     3121 ttttttaggc caagtaaggt gcgcagcacc gcatccggca acgttacacc gactccagcc
     3181 gggcataagc cgccaccagc catttaatac cctggccctg aaatgccacc tgcaaacggc
     3241 tatgctcacc gctgccttcc atattgacaa tggtgccttc accaaactta gcgtggcgta
     3301 cgcgctggcc gagcttgtag ccgctgtcgt tctcgaccat cggcgtaccc atccgctgat
     3361 ggctgaccgg gcggcttacc gtggcgcgca ggcgcacctc ttccacacac tcttccggca
     3421 gctcgccaat aaagcgcgac gggcgatggt aaacctcttt gccatacaga cggcgagttt
     3481 ccgcgtaggt cagcgtcagt ttctgcatcg cgcgggttac gccaacgtag gccagacggc
     3541 gttcttcttc cagacgcccg ccttcatcca gcgacatctg gcttgggaac atgccctctt
     3601 ccataccgac gataaacacc tgcgggaact ccagaccttt cgccgagtgc atggtcatca
     3661 gttgtaccgc atcttgccag gtatccgcct gcccttcgcc tgcttccagc gccgcatggg
     3721 ataggaacgc ctgcagcggc attaaatctt cgtcttcttc gttgtagctg aactggcgcg
     3781 ttgccgtcac cagttcctct aagttttcga tacgcgtctg acctttttcg cccttctcct
     3841 gctcgtacat ggtacgcagg ccggagtctt taattacccg gtcagtctgt acatgcagcg
     3901 gcatatcggc agtttcctgc gctaaggcgt cgatcagttc cataaaccgc tgtaaggcgc
     3961 tggcagcacg tccggcgagg gctttttcct gcaacagttc acgacatgcc tgccagagtg
     4021 ttaactggcg atcgcgcgat gtctgacgta ccacgtccag cgtccggtca ccaatacccc
     4081 gcgttggcgt attcaccaca cgctcaaagg ccgcgtcgtc gttacggttg gcaatcaggc
     4141 gcagatacga gagcgcatct ttgatttcct ggcgttcgaa gaagcgcatc ccgccgtaaa
     4201 tacggtacgg catactcgcc tgcaacagtg cttcttccag cacacgcgac tgggcgttac
     4261 tgcggtagag aatggcgcac tcggcaagcg ccccgccgtt gtcctgccag gttttgatgc
     4321 ggttaaccac aaaacgcgct tcatcgagtt cgttaaaagc gcaatagagg gaaataggct
     4381 caccgtccgc gccatcggtc cacagttttt tacccaaacg cccgttgttg ttttcaatca
     4441 gggcgttcgc ggcgctcaga atattgctgg tagagcggta gttctgctcc agacggatag
     4501 tttcagcacc ggggaaatca ttgaggaaac gctgaatatt ctccacctgc gctccacgcc
     4561 agccgtagat tgactgatcg tcatcgccaa cgatcatcac tttgccggta tcgcccgcca
     4621 gcaggcggat ccacgcatac tgaatgttgt tggtatcctg gaattcgtcc accaggatat
     4681 tggtaaaccg ttcgcgatag tgctgaagga tatgcggctt gttaagccac aactcgtgag
     4741 cacgtagcag cagctcggcg aagtccacca ggcccgcgcg gtcacacgct tcctgatacg
     4801 cctgatacac cttctgccag gtctgctcca ccggattacc gtagctttga atatgatgtg
     4861 gacgcaggcc ttcatctttc tggctgttga tgtaccacat tgcctggcgc ggcggccact
     4921 gcttctcgtc gaggttcatc gccttgatta ggcgtttgag caggcgtagc tggtcctcgc
     4981 tgtcgaggat ctgaaaatcc tgcggcagat tggcgtccat atggtgcgca cgcagcagac
     5041 ggtgcgccag cccgtggaag gtgccgaccc acatgccgcc ctggctggtg cccatcagct
     5101 gcccgatacg atgacgcatc tccgccgccg ctttgttggt aaacgtcacc gccataatcg
     5161 agtatggcga gcagttttcc acgctcatca gccaggcgat acgatgcacc agtacgcgcg
     5221 tcttaccact gcccgcgccc gccagcacca gaaggttgct gcgtggcgcg gccaccgctt
     5281 cgcgctgttt gtcattaagg ctgtcgagca ggtaagaaac gtccattggc accgccgcgt
     5341 aaaaataggt tgggcggaga agcgcaacgc cctccgccaa aaagctgggt atatatacag
     5401 atttgctgat tatatcagcg aggtcagaga tgccaaccgg gaaatttcca gatgcggcag
     5461 taaacggctg tcccaggttt gcatcagatc gccattttcc ggtctgatcc aacaggcctg
     5521 cattccgctg cgaattgccc cacccacgtc agtggtgaga tcgtccccaa catgtaagat
     5581 ctcgccgatc ggcacgttga gtttttccgc agccagaaag tacatatcgc tgaacggttt
     5641 tgagcgcccg tgcgggccag cgcgcagcac aaactcaaaa taatccccca aaccaaacag
     5701 ttccggctgg gcgttaccgt tggtgatcgc caccagcggc catttcttcg ccagctgttt
     5761 taaggtgtcg tgagtttgct gcgggacgtc gattcggctg cgccatttgg caaagttgat
     5821 cattgctgcg tgtgcgcctg cactggcttc ttcggcactc agcccggcgt cgagcatcgc
     5881 ttgttcaatc gaacgaaaac gccagcgcgt cacgtcgtga taaatctcgg gttccacttc
     5941 ccgtaccgcc tggcgcaggc gttgcagatc ttcattctgg aagctgcgca gcgccggatg
     6001 ataattttgc acaaaggtaa gcgcctctcg ctcggtgcgc aaaatcaccg gacggttatc
     6061 gtaaagggta tcatccaggt caaaggtgag cgccgagatg cgccccaaag gccggtaaaa
     6121 acgcattatt tcccccgttt ggcgcgtgga tgcgccgcat cgtacaccga ggcaaggtgt
     6181 tgaaaatcaa gatgagtata gatttgcgtg gtggagaggt tggcatgacc cagtagctcc
     6241 tgcacaccac gaagatcgcc gctcgactcc agcatatgcg tagcgaacga gtgacgtaat
     6301 ttatgtgggt gaacgtgatt attcagccct tgttttatgc cccattcggc aaagcgtttc
     6361 tgcacattac gcgcggagat acgcttgccc agtttcgaca gaaaaagcgc gtcgtcttcg
     6421 ctaccaaaca ggtcgcgcaa atcaagccag tgctcaatcc acgccacagc gttgcgacca
     6481 atcggcaggc ggcgctcttt gctgcctttc cccataaccc acacttcacc agactccagg
     6541 tcgaggtgtt taatatccag ccccaccagc tcagaaagac gcagacccgc gccgtacatc
     6601 acttccagca ttgcacggtc gcgtacagcg aggggatcgt tgatatcaat atccagcagc
     6661 cgattcatat cgtcgacgtc gatgtttttc ggcagatgac gcggcgcttt cggtgccgaa
     6721 acacctttcg ccgggttagc tttgagttcg ttctggctga ccagccagtc aaaaaagcta
     6781 cgtagcgcag aaagccgtaa cgccagactt gctgctccca gccctttacg gcgactgcgt
     6841 acagcaaaat tgcgcaccat cgtcacatca cattgctgcc agctttgcag gccgttttcg
     6901 ctggcaaaat tgatgatcgc ctcaagctga cgctggtagt taagcagggt tatcgggcta
     6961 agctggcgct ccacgctcag ataacgtagg tagcgttcta catcggtgtg taaatcggtc
     7021 atacgcgttc aatccaacgc tccagaagct ccggcaacat cagcgcaatt tcatgaagta
     7081 actgcgttcc ttgcccttgt tgatagtgac tggcatcgcg actggtaaac agcacgacac
     7141 ccaaatcagc atcgcttccc agcatcgaca tcgccaccga tcccaccgct ttcgcttccg
     7201 gtagcaccac cagcagctct ggtccgttaa gcggcccaag atagtgctgt tcctgcccca
     7261 aacgctgaat acgcagcggt tcgaaagact gacggcttaa tgccagatga gtgtggttcg
     7321 acggcgcacc taagcgccag cgatccggaa acaggcgcag actcgcacct gccaggccga
     7381 gatcgcgcgc ccagcggtga aagcgcatca gcatatcgtc gagactgctg gcggcggtga
     7441 gactgcgctg caggtagagt aggcgataaa acaggccttc gttggcgata gcctgttcca
     7501 tcaacagcgc catgttctct tccagaacat gaatatgatt acgtgcgcgg gccatgtgcc
     7561 actcgaccaa cgaaacggtg ccgcgtaccg gatgcggcac acgtatcgct tctactgcgc
     7621 gcgcattacg gataaaaaac tcaggatttt taatcagata atcgacaacc gcccggtcat
     7681 caagctccgt gagtgtttcc tgcagttctt cccctggttg cttcatagat gaataaatcc
     7741 gtcgtagaca tgtaccgccg ggccagtcat atataacggg tgacccggac ctttccaggc
     7801 gatatcaaga cgaccgccgg ggagttccac gcgtacttct tcggccagca aaccttgctg
     7861 aatccctact gcaaccgccg cacacgcgcc gctgccgcag gcctgggttt ctcctgcccc
     7921 acgctcataa acgcgtaaac gaatatgctc gcgcttaacc acttgcataa aaccgatatt
     7981 ggcgcgctcc ggaaaacgct cgtggctttc cagaacagga ccaagcgttt ctaccgccgc
     8041 ggtatcgaca tcatcgacct gaatcacgca atgcggattt cccatcgaca ccacgccgca
     8101 taagattgtc tgctcggcgg cgcgcataat ataggtcttt tccgctttgt tagcgcgaaa
     8161 cggcacggcg gaaggttcga agttgggttc gcccatattt acgcggacca gatcatcatc
     8221 ggtgacggtc agaaccatcc gcccgttggc ggtgctgacg cggatatcac gcttattggt
     8281 cagtcctttc agacgcacaa aacgggcaaa gcagcgcgca ccgttgccgc actgcgccac
     8341 ttcactgcca tcagcattga aaatgcgata gtgaaaatcc agttcaggat catacggcgg
     8401 ctcaaccacc agcagttggt caaaccctac ccccaggtgc cgatcagcca ggcgacgaat
     8461 cagctccggt gaaaaaaaga cattctgcgt taccgcgtcg acgaccataa aatcgttgcc
     8521 aaggccatgc attttcgaga actgcatcat ttactccaat cacgcgggta cagaaactga
     8581 cttttagtaa ttcacctggg atggaccatc gccagtggcg cgatcgtttt tatccggcac
     8641 cgtggattgc gtttgcgtct ctaccggttt ggtcggcggc ggtgcgtttt tatctgcagg
     8701 cgggaaatag agcggacctt tcagaccgca gcccgtcagg ctgaagagag taattaatac
     8761 agtgagtgcc ttaaacacgt ttttcattat ggattgcctg taaagttcat cgctttctgc
     8821 tttctatcat cgcaggagaa gacgtaaaag caagcgttta gcgactcttt atgaagtctg
     8881 aaaagtgagg aaatgctgcg agtataacgc ttactcgtgg ggatttatgt tttgctggta
     8941 gtttcgtgat cggcacctcg cgccaggatg gctctatgca ctatcgcaat gtagtttgtt
     9001 tgttggcttg ttcgttgttc ttatcgtcgg catggagctt ttaaaagagt ggcgcaccat
     9061 gcaattttgt tgtctgacct tagtcgccag tgacatacaa ctttgccaac ctggtgataa
     9121 gagggaataa aatctgatgg ggctatttac ccaactggaa aaatttgacc aaaagcttac
     9181 gcgcggttat gcccggtggg ggcgctgggt atggcggacg ttaattgtcg ttccggtact
     9241 ggtcgtgttg tggaatatag gacaggcagt atggggagga ccaagaggtg gagtgattct
     9301 ggaaatccac agtgaaattg accgcccgat actgggattc agtgttaacg gcgtggcggg
     9361 cgcgaatgct tttgcaaatg gtggcggttc aacaacctgt tgtggtgatg ttagtgggga
     9421 tacggcagag gttatctgga cattaagtac tactcgtaca cagtataatg ctggaatgcg
     9481 tctggagaaa cggaatatga cgttacctct ccccaaaaga gaatggggag aagattttct
     9541 gcatgtgcat tttatgcctg gcgataaagt gctgctgggg tggagtaaag attctttttc
     9601 tccgtatgaa gatcttcaca acggggggta caagacccgc gttcggcagg atgtgaaaga
     9661 taaactttat ggtacaggga aaatgaattg atagatttct ataaaaatca cgttcggcag
     9721 aaagcgtaat aatgtgggga atgttagata ccggtggtgg gacctaagta caacacgcga
     9781 aagacaataa agtaggtaac ggtgggcaga gtcgcatgtt gacacccttc ccggcgtcaa
     9841 tccggcacat tttaacgtga tcagtcacac catggcgcag tagctcgtat ttatatcgtg
     9901 aatgaaaacc tcaagtttac cccagcaact atcagaaaat agcgatggcc tttatagatt
     9961 ctgaaaagtg acgatttgcc gcaaaagtta cgcttacgtg cgaggaatta tgttctgctg
    10021 gtagtttcgt gatcggcacc tcgcgccagg atggctttat gcactatcgc aatgtggttt
    10081 gtttgttgtc ttgttcattg tttttatcgt cggcatgggg atgtcggctt gatccgcctg
    10141 aacataatat ttaccagaaa cagggaaaag gcgtggtata cctgcgccct tataaagaaa
    10201 caaatctttc tcttcctgac gtgaattaca aaagtttgcg ccgtttaccc aatttattaa
    10261 tagacccgac aacactagac aaatgggata aagtacctcc tctgactgat ctgacaaccg
    10321 actacctcta tgaaggcgca caggcctggt atcctcatta ctcctggcat agcgatggtc
    10381 gctatatcct ctacgcgggt gaagttgtgc aaaacccgcc agacaaacct cccgtcgatg
    10441 tcgcctcgtt caaggcgtgg ggtgattttg ccgcagataa acacagtctc tatttcgaag
    10501 gcaagcgcac cgataacaat ggcgggggaa atcgcttaga tatcaaaaca ctgcatcagg
    10561 tggaattccg gcctccctgg gatcctgact tactggggct gatattacgt gacgctaatt
    10621 tcttgtatat caatgggcat cgcctcgcag atcccgatag tttccgtgtg ctggcacaaa
    10681 agtcatggga tcagcgagga aagttttcta ccgcgtttaa tccctgtgtg gctatgccat
    10741 ttggcccctg ggataccctg gctcgtacac ggaccaaaat cctgatcaac ggcgaacagc
    10801 ttgatgccga cccggacacc ttttccgtcg tgcgctggat gcccggctca ctcttgacct
    10861 ggcgtgataa aaacgggctg cagcgtaaag tcctcgacaa ggaaaatctg gagtgggatg
    10921 aagatttaac aaagcactgt ctggattttt ctctgctgga aaagaaagtg ttctggcgta
    10981 aaggacctgc ttgtaaacag gaagaattac ccggactcga tccggagcag tttcaaccca
    11041 tcagtgatgc tgtcgcccag tatcaggact cgctttatac catcatcgaa acagagtctg
    11101 gtgaccgcaa gctggaaatc gtgaaacttg atgatcccaa tcttattatc aacaaacgtt
    11161 tcaacgccgg gaaacgccac ggctatttac ttacgcgtgc cgaagggtgg ccataccatt
    11221 ccggtttaca tgtgtttgaa tctgacggac cgctgatctt actggataac cgttctccgg
    11281 atgaacgcga agcccatctt aatgaccatc cctttttgcg cagatggtat gcccgcgata
    11341 accgctacat ttacagcttt gatggcgcgc agctctggcg ataccgcacc gctgatccga
    11401 aacaagttcg cttaatctgg aaggaacaac attcgggata tggctatggc gtaaattaca
    11461 aaacgggata tctggacgga aaaattactg atgacggcga atttattcct gccccgcgca
    11521 atgaggcgac aaaatgaagg gtcaaataat cacagttgca ctcatcctgt tgggacacac
    11581 atttctgacg cctgctgtac aggccatagg gttcgactat tacaatgatc acggtgttat
    11641 gtcttacggt aaaggttatt ggggggataa gaaaatcatt gcccagtttc cggagatgag
    11701 cagggccgat ttgcgccttg tgacaaatat atccggggag cgggagttgg tggaaggcta
    11761 tatacccacc gatgaaataa gcataaaaaa tgaggaatac cactgggtga cagacggccg
    11821 cgttattctc tggcgtggca aaatcgttag caatccaccg ggaacaccca ctgtcgatat
    11881 tgccagcttt caggctatgg gccgcttcgc ggtcgataaa tatagcctct attttgacgg
    11941 acagcgcact gaaagtaata gcggcgcgtc acgtgttgat ctggcgacac taaaagctat
    12001 cgaaggtaac tctaccacgc tggtggatag taaaaatctc tatttgtccg gtcgacgtca
    12061 ggggagcagc agtaatgtta ctgtattaga aaaaagatgg tggggtatta atccacgtct
    12121 tatgagtgta aacagaaatt tgtattccaa tgatctgctt atccgcagtg ggcagaatat
    12181 ttatttaaat ggcgttcacc ttacggcgaa tgcagactca tttgagataa ttcgctggat
    12241 acctcattca ctgctggttt ttcgcgacaa taagggtatg catcgttatc cctttgggca
    12301 attgtctggc aaagcgatac ccgtagatga tgacgtctct tttgaagtag gggaaagtcg
    12361 cgttcgctgg cgtaaacagc tcacgtccga ccgtcagtgg agcaagtgga tagacctacc
    12421 agatattgaa cctgaacaat tccatctgat tactggcaat attgcgcaat ataaagatcg
    12481 gctgtatgta acaaaattat cgacatttgg tgaagaccag cttgagataa tcccgctgga
    12541 tacgccagac ctggtcattg atcgctcatt taatagcggc aaacagcatg cttattttat
    12601 ccgccaatta cggtcaaaga gcgtgcaaat tattccagtt aacggtccgc taactaaaaa
    12661 cgatcgcttc gcttatgacg atcgcaatgt ttatacatgg accgatacag aggtaaggat
    12721 tacgccctcc ccctgcccgg cgaaaacaca cgtcagagaa gagaatgtgc gtgaacttca
    12781 caacagagac attattattc cgttgacgga tgactcatgc cggaacgcag caacagacgg
    12841 gcaaactttg aagccctgac cccataccgc tatactgccg ccatcactta aagacaggat
    12901 acaacaatga acgacagtga atttcatcgc ctggctgatc aactctggct gaccattgaa
    12961 gaacgcctgg acgactggga tggcgacagc gatatcgact gcgaaatcaa cggcggcgta
    13021 ctgaccatta cctttgaaaa cggcagcaaa atcattatta accgccagga gccgctgcac
    13081 caggtgtggc tggcaaccaa acagggcggc taccattttg acctaaaagg cgatgaatgg
    13141 atttgcgatc gcagcggcga aaccttctgg gatttgctgg aacaggcggc gacacagcaa
    13201 gcgggtgaaa cagtcagttt ccgctaagat tgcatgccgg ataagcctcg ctttccggca
    13261 cgctcatcac gaaaaatact gctgtaatag cggcgtatcg ttgtcctgat tggcgggcgg
    13321 catgttaccg atagattttg tgcggaacgg aatcacctgt tcacgaccat caaccttcac
    13381 aatctgatag aactgcggca ggttgaagtt gataaagctt gagccgtagg tgaagcgatc
    13441 gtgcgatgac gagtagaagc gactgacgtc acgtaccagc tcctctttgc tgccttcgca
    13501 gtggtgatac acctcaaccc ggttgctttc gtcgagaatg tagatattaa agccattctc
    13561 gtcttgcgtt tcttcgaaga agaactggat gatcccttcg ctggcaaagc cgtccaccac
    13621 cgccggtaat ttgacgtgat tggtttcaac ctgcactgac aagccgtgca gtttgttatg
    13681 cgaaatagcg ccataaaact cgatggcgtt ttccagtttt tgtaccgata cattcaggcg
    13741 ttcgaagaac aacccccagg tttgaccaga aacgcgcagc gccttgaaac gcccggtttc
    13801 ctggcgggtg ctggaaaggc gcagttcaat acactcagaa accagttgct gcacgcgagt
    13861 acggattaac ccgcgcagat gctggctata acagaagact tccacgctat ctggcggtgc
    13921 ggcgtcctga tgcattttgc cgagaatagt tttcagggct tcgatcatcg attgctcgcc
    13981 gttgaagtgc agcgtacgca cttcgttcca cgagttgcgg tacagcaggt caacgctacc
    14041 taccaggcaa ttttgattct cgccaaagct gaagacatcc agcttacgga aatcaaaatg
    14101 caccacctga ttgcggaacg ccgctgtcgg gtcatattcc aggttaacga taatcgccag
    14161 atggcggatc tcacacgggc tgtagagcgc cttcggtgtc ggtgcaggta agcgcagcgg
    14221 gaaatggtgc gacacgtcgg cgaccatctc ctgcaactta ggcaaatcga caatgccgtt
    14281 acctttaata tacaaacggg tgcgcgaggt cagcaggccg ttaaaccatg cccacgccac
    14341 cagtttgttc aggtaacggt tatattccag cggctgatgg ctgatgatcg actcaatatt
    14401 tggcgcgcgg ttatacagat accaacctga acggttagca cggcccggcg gcacataaat
    14461 aaaggtcaga ttcggttccg agagatcggg tgaaatctgc gggtttacca gcgtcacttt
    14521 acctggtaat gcttcaaacg cggcatacag cttacgcgtc agcacgccga tatcctgcgg
    14581 actggcggag acgctaaggt tattgcgacg cgcaaagcgg atcagattac ggtagctctg
    14641 catcatcgcg tcgagcaact cgttgtgcgc ctcacgcacc tgatcaatct tccagttagc
    14701 gcggttatcg agcattgcca gacgagcttc gtcccaaccc cactcgctca ctaactggct
    14761 caacactgcg cgacgccagc ctacgcaggc gcgttcacgg ctgagctttt cgcacacttt
    14821 taaatagaag cagcgacgta ctaaatccag acgggtaaaa tcttcaatcg ccgtcaggta
    14881 ttcagtaaca cgctccagca tcatgcagta tggatcgaga ccaaacgata caatctcgcc
    14941 gtcgtgcaaa cgctgtttga tatctttcgc cagcagacgt gggttcgggt attcccagga
    15001 ataggcttcc agcagcagtg ttttcagtac cgctttgtat ggggaatcga tactcttgta
    15061 gagctgccaa aggctggcac caaagtactc ttcagcagaa agcgagctta agccaccgag
    15121 atccagccat tcatttggcg tcagcacgcc ctgcgcgtaa agcgtcatca catagtcgtc
    15181 gtaatgctct tcttcgtcgc acggcaccat attccacaga atacgcttac cggcgagacg
    15241 cacggcggta cgataaaatt cgtcaagcag cagtatatgc tgggtggagc cacaatcttc
    15301 gccccccagg ctgccgcttt cattatgacg gaagcggttt tcatcaatca ggaagaagct
    15361 gacttccaca cccagcgagg cggcccagct ttccagcaga ctacatttac gttgtagtaa
    15421 ctggcgctct tcgctatcga gccaggattg atgacagacc cagatatcca ggtcagagga
    15481 acaactttgc cctacggacg aggtgctgcc catggtgtat acaccagtaa ttggaagctc
    15541 acctttcggt gggtcctgta ctgacattcc acgatacagt tcaagctcgt tcaggtagtg
    15601 gcgttgagtt tcatcaggcg tgtaaaggca aatgcctttg ggaacgttac catcaaggta
    15661 acccggcatt agcggatggt gatagtgcaa caatgtcggc agtagactgt agacctgttg
    15721 gaatgcaggc cccatagcag caagcgcgcg atccacacgc aattgattta tggcatccag
    15781 tctctgtttc agagtctcaa tatagaggta caagacgtat cgcctgattt gctacccgtc
    15841 atgactgtga ttccgccaac atcaacggta acacgcggca ttcgggatat ttcgtatgtc
    15901 aaaggtaacc gttaccactt ttcgcgcctg gtttttttag tttcacgacg aaaaaatggt
    15961 ctaaaacgtg atcaatttaa caccttgctg attgaccgta aagaaagatg cgctacatac
    16021 aagtgtagca ccgtttattc tctgtaaatt ccttattacg acggcgtgaa acgcctgtca
    16081 ggatccactg ccagacctca ttttacggtt tgcgcaggcg tctacgtttc accacaacac
    16141 tgacatcact ctggcaagga tgttaggatg gaccacggat gataatgacg gtaacaagca
    16201 tgttagacaa tgttttaaga attgccacac gccaaagccc acttgcactc tggcaggcac
    16261 actatgtcaa agacaagttg atggcgagcc atccgggcct ggtggttgaa ctggtaccga
    16321 tggtgacgcg cggcgatgtg attcttgata cgccgctggc gaaagtaggc ggaaaaggct
    16381 tatttgtaaa agagctggaa gtcgcgctcc tggagaatcg cgccgatatc gccgtacatt
    16441 caatgaaaga tgtgccggtt gaattcccgc aaggtctggg gttggtcact atttgtgagc
    16501 gtgaagatcc tcgcgatgcc tttgtgtcca ataattatga cagtctggat gcgttaccgg
    16561 caggcagtat cgtcgggacg tccagtttac gtcgccagtg ccaactggct gaacgccgcc
    16621 cggatctgat tatccgctcc ctgcgcggca acgtcggcac tcgcctgagc aaactagata
    16681 acggcgaata cgatgccatc attcttgccg tagccggact aaaacgttta ggtctggagt
    16741 cccgcattcg cgccgcgtta ccacccgaga tttctcttcc ggcggtagga caaggtgcag
    16801 tcggtattga atgccgcctt gatgatacgc gcactcgcga gctgcttgcc gcgctgaatc
    16861 accacgaaac tgcactgcgc gttaccgcag aacgcgccat gaatacccgt ctcgaaggtg
    16921 gatgtcaggt gccaattggt agctacgccg agcttattga tggtgaaatc tggctgcgtg
    16981 cgctggtcgg cgcgccggac ggttcgcaga ttattcgcgg tgaacgccgc ggtgcgccgc
    17041 aaaatgccga acaaatgggg atttcgctgg cagaagagct actgaataac ggcgcgcgcg
    17101 agatcctcgc tgaagtctat aacggagacg ccccggcatg agtatcctgg tcacccgccc
    17161 gtctcccgct ggagaagagt tagtgagccg tctgcgcaca ctggggcagg tggcctggca
    17221 ttttccgctg attgagtttt ctccgggtcg acaattaccg caacttgctg atcaactggc
    17281 ggcgctgggg gagagcgatc tgttgtttgc cctctcgcaa cacgcggttg cttttgccca
    17341 atcacagctg catcagcaag atcgtaaatg gccccgacta cctgattatt tcgccattgg
    17401 acgcaccacc gcactggcgc tacataccgt aagcggacag aagattctct acccgcagga
    17461 tcgggaaatc agcgaagtct tgctacaatt acctgaatta caaaatattg cgggcaaacg
    17521 tgcgctgata ttacgtggca atggcggtcg tgagctaatt ggggataccc tgacagcgcg
    17581 cggtgctgag gtcacttttt gtgaatgtta tcaacgatgc gcaatccatt acgatggtgc
    17641 agaagaagcg atgcgctggc aatcccgcga ggtgacgatg gtcgttgtta ccagcggtga
    17701 aatgttgcag caactctggt cgctgatccc acaatggtat cgtgagcact ggttactaca
    17761 ctgtcgacta ttggtcgtca gtgagcgttt ggcgaaactc gcccgggaac tgggctggca
    17821 agacattaag gtcgccgata acgctgacaa cgatgcgctt ttacgggcat tacaataact
    17881 ctcataacag gaagccataa tgacggaaca agaaaaaacc tccgccgtgg ttgaagagac
    17941 cagggaggcc gtggacacca cgtcacaacc tgtcgcaaca gaaaaaaaga gtaagaacaa
    18001 tactgcattg attctcagcg cggtggctat cgctattgct ctggcggcgg gtgtcggttt
    18061 gtatggctgg ggtaaacaac aggccgtcaa tcagaccgcc accagcgatg ccctggctaa
    18121 ccaactgacg gcattgcaaa aagcccagga gagccaaaaa gccgagctgg aaggcattat
    18181 taagcaacaa gctgcacagc ttaagcaggc gaatcgtcag caagaaacgc tggcaaaaca
    18241 gttggatgaa gtccaacaaa aggtcgccac catttccggc agcgatgcta aaacctggct
    18301 gctggctcag gccgattttc tggtgaaact cgccggacgg aagctgtgga gcgatcagga
    18361 cgtcacgacc gctgcagcgt tgctgaaaag tgcagatgcc agcctggcgg atatgaatga
    18421 cccgagtctg attaccgttc gtcgggcaat taccgatgat atcgccagcc tttctgcagt
    18481 ttcgcaggtg gattatgacg gcatcatcct taagcttaat cagctttcaa atcaggtaga
    18541 taacctgcgt ctggccgata atgacagcga tggttcgccg atggattccg acggtgaaga
    18601 gctttccagt tccatcagcg aatggcgtat caatctgcaa aaaagctggc agaactttat
    18661 ggacaacttc attacgattc gccgtcgtga tgacaccgcc gtaccgctgt tagcgccaaa
    18721 tcaggatatc tatctgcgcg aaaatattcg ctctcgcctg ctggtcgcag cacaagctgt
    18781 accgcgtcac caggaagaga cttatcgcca ggcgctggag aacgtctcca cctgggtacg
    18841 agcttactac gatactgatg atgccaccac caaagcgttc ctcgacgagg tggaccagtt
    18901 aagccagcaa aatatctcga tggatcttcc ggaaaccctg caaagccagg cgatgctgga
    18961 aaaactgatg cagacccgcg tgcgtaacct gctggcacaa ccggcagcgg ggacaacgga
    19021 agctaaacct gcacctgcac ctgcacctgc acctgcaccg caaggtgata ctccggcagc
    19081 cgcgccgcaa ggagaataac catgctaaaa gtgttattac tctttgtttt gctgattgcg
    19141 gggatagtgg ttggcccgat gattgccggt catcagggtt acgtgctgat ccagactgac
    19201 aactacaata tcgaaaccag cgtcacgggc ctggcgatta ttttgattct ggcgatggta
    19261 gtgctgtttg ccattgagtg gctactgcgg cggatcttcc gcacaggcgc acacacccgt
    19321 gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc agaccgaaca ggcgctgctg
    19381 aaactggcgg aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac
    19441 gcggaacaac cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat
    19501 gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc tggccggcaa tgacaccatt
    19561 ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc
    19621 cacggcgtgg ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg
    19681 gaacaggcgt atatccgcac aggtgcatgg agttcgctgc tggatatcat cccatcaatg
    19741 gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt
    19801 ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa ctggtggaaa
    19861 aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg tggcaatggc ggaacatctt
    19921 attgaatgtg acgatcatga tactgcccag caaattatca tcgatggcct gaaacgccag
    19981 tacgacgatc gcctactgct gccgattcct cgactgaaaa ccaacaatcc ggaacagctg
    20041 gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca
    20101 ctgggccagt cactgatgaa gcacggagaa tggcaggaag catcgctcgc cttccgcgcg
    20161 gcgctgaaac aacgtccgga cgcctacgat tacgcatggc ttgccgacgc gctggacaga
    20221 ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag
    20281 aacaacccac cacagtagtt ccttctcacc cggaggcaag cacctccggg acctttctga
    20341 tgcataaaaa aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa
    20401 caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct
    20461 gtcagtggac gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta
    20521 aggcgaacat aagagatgga atgagcatct acccgtttat tatgccacag agaatcggga
    20581 aataacatcc cttaacactt gttatgagat aattctgtaa tcctctttgc ttcctgagta
    20641 ataacttcct gagtgaatat ttaacctgag cttgatccta cacatactta ttatgaatga
    20701 taaaattcat tcaattaata acacatatat taattgccgt taaaactaaa aaaagcatca
    20761 ataatcaacg cgatataata aacctgcctt acatatcaac tgcgccagag gtaggattga
    20821 aaacgctctc ctgattttcc aattcatttt ctggataaat aaataattta tttttgtcac
    20881 tattatttat gtaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg
    20941 tcatcatttt gatgacatga aaccggagca ttttgcatgg aattttcgtt ttcacccaaa
    21001 cttcttgttg ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc agataccccg
    21061 tcaactgcca ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatccacag
    21121 aacgctgcaa tttattgaat ttgcacgatc atgtaggccg gataaggcgt ttacgccgca
    21181 tccggcaatc aaccgcaggc ggccacaaaa tggccgcttt tttacttact caccaccagc
    21241 aaatgcgcat gcataatgtc actggccggg cgaccgtgcg ccagcaaatc agccattgct
    21301 ttaagatatg gcggtagatg gcggaaataa cgctgatacc cggcacacaa ataattcagc
    21361 cccggtttgc cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct
    21421 tttaacacgt tacaactgcg acactgcgcc ggtaattgct taaatttatc ttcaccaaac
    21481 acctgctgtt gcggggaatc gatcatttct gcaattgttt gctgatgcat attccccagc
    21541 cgatattgcg gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc
    21601 accgagcgcc cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg
    21661 gcaaacgccc attcgatatt catcacgaaa atcttgccga cgtcgcgttt gatccagtgg
    21721 tcgaatatcg ccaccagaaa ttcaccaaat tcatcggggc gcaccgacca ttccgtcagt
    21781 tccccctgaa tatcaccagg cgcatgtagt ttcagtccat cgctggcagc tgtttcatca
    21841 gccaggcgct cgaccaccgg aataaactgg atgaattcga ctcccgcatc gcacaaaaaa
    21901 tcataaacct gcaacggttg ctgcgcgctg gtgcggttga cgcagaccag cacgttatag
    21961 tcgacatgat gtttttgcag cagcgtcagg gcacgcatca ccagcttatg cgttggtctg
    22021 ccacctttgg tcacgcgata ttgattgtgg atctcagccg gaccgtccag cgataaccca
    22081 acaagaaaat gattttctgc cagaaatgca caccatttat catcgagcag cacgccgtta
    22141 gtctggaagc tgttacttat cttcctgcca gcaccgtatt tcgcctgtaa ctttacggca
    22201 cggcggtaaa aatccagccc gagtaacgtt ggttcgccgc cctgccaggt aaaagccact
    22261 tcgttttgcg gttcgctggc agcgatatag tgacggacat acgcttccag cgtgtcatcg
    22321 tccatatgcg tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag
    22381 tcacagttca gattacaatc ggaaccactc ggtttcgcca tcacatgaaa agcacgcgtt
    22441 ggaacctgtt gcagcatggt cactccttgc ctgaagtcat ccttcaatac gttaagggcg
    22501 aaatcatacc gtggggcaat gagttattac gggcgtttat tgcgaaggga gggtgacgaa
    22561 atgcacagaa aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga
    22621 ttcgaacctc cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg
    22681 aaatactact ttttgaattt ttagttcaat tctttaaagt cgtggtgcga ggggggggac
    22741 ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc
    22801 caccttcgca ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac
    22861 aactggaatc acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt
    22921 tacgcggtat tactaccacc gcagctcaag cgccgggact aaatggcgcg cccgacagga
    22981 ttcgaacctg agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc
    23041 ttagcgccgt tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt
    23101 aaataaattg cgcgtttggt tacggtttgt gcgttttgct gattattcct ccagtttatg
    23161 cgctttcccg tggcgattaa ggccaaaaac tttataaatc gccgtcaccg ccagcataaa
    23221 gatgatgcca acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac
    23281 gcaaatcagg aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg
    23341 atggctggca atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac
    23401 aaaccatggc accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg
    23461 cggattggga atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc
    23521 cacacccgca actggtacgc cgtgacggga aactttcgcc atcgccgccg gtaactgacg
    23581 gtttttcgcc agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga
    23641 gagcgcagcc gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt
    23701 ggcaaaagtc agtacgaacg ggctgccgtt gctgccaatt tcattccacg ggaagatggt
    23761 gacgataacg aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac
    23821 ggcactgcgc agcgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc
    23881 cacgccctgg taggacgcca ccacaataca cagcgcggtc aggaaccctt tccagccacc
    23941 cgcaaagaaa ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc
    24001 aaagccaaag aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt
    24061 gatcatcgcg aaccagaact cgatttcgcc gtacaaccgc accgccgcca gattcgccaa
    24121 cgccaccagc gccactgcga tcaatgcggg tatccactgc gccatctccg ggaaccagaa
    24181 ctggacataa acgccaatgg cggtgatttc tgagatcccc accgccatcc acataaacca
    24241 gtaagaccag gcggtgagat aaccaaagaa cgggctcata taacgatgcg cataaacggc
    24301 gaacgaaccg gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa
    24361 gacgaatagc ccggcgatga tataggccaa caatacggat ggcccggccc atttcaaggt
    24421 actggcggcc cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat
    24481 atgtcgagct tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt
    24541 tgtgtttgca tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa
    24601 tgttttcgta attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta
    24661 ataaaaaagc agacaggcga cggagtgacc actccgtcgc tttacaaaga gaggaaaatc
    24721 agaggttgcc ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc
    24781 ggctcggctg cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg
    24841 gtgcgcgttt tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg
    24901 catctggatg caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg
    24961 caacggtgac gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct
    25021 cgggtttaaa ataaccatcc tgactgccaa cgatattcac attccactgg ttgcgcagtt
    25081 tagcttcagt ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt
    25141 ctttgcctgc gcgcgccatc agctcttccc agagatcagc accggcaacg cgggaaacct
    25201 gcgcctgcgg gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa
    25261 attcggcagc gttaattagc tccctgacct cagcattatc ttcaatggtc agcatttttt
    25321 cggcattaat ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga
    25381 gggcgtgctg catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg
    25441 ccatggtgtt gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg
    25501 tttatgaatg agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca
    25561 tgcgccgaag accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg
    25621 tgccagcacg atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt
    25681 atagcgatta ggctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag
    25741 accaaccacg atcgccccga gcgggatgaa caacgcgccg cccatcacca ccagtgagcc
    25801 tataagcgta ggcgagatcg ccagtccgga gtggttattc agcacttccc aggtaaagta
    25861 gttggctgag ttcagcacca tactcgggcg acccggccac agccaggaag ggataaagac
    25921 atagaaatcg cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa
    25981 cagcaacgcc agattctccc acggcgagaa ggtgtcgcga gtgagataga gaaacgtata
    26041 gaacgcttca tcgccgctca cattcattcc atagcgtttt agcgccagcc agaacatgcc
    26101 cacaatcccc agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat
    26161 gccaataaac aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat
    26221 catataagtc agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct
    26281 atcctggcgc agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt
    26341 taacgccacg ccggagactt cactggagaa gatctgactg taggagttaa gccggaacag
    26401 caaaaagccg ttgtgcatga agaagatgcc gacgcttacc agcgcgatgc ccatcaggat
    26461 cacccacgta agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc
    26521 agcaacgcgt ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc
    26581 cgcagaaagc aacgcctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa
    26641 taccagcacg ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa
    26701 gaagacattg aagttaaagc gcacacggcg aaactcaaac caggtcagcg tggcaataaa
    26761 cagcgtgcag agcagccaga ccacaaacag gccactgaat tgcagcagac tcatgcgccc
    26821 tccccggcgg caatcgccaa cgcccgctgc cagccttgta gatagttagg gctaaagaag
    26881 gcaatggtgt ttttatccac cgacgccaac tggcgctgcg cttcacgcac aatatcctcg
    26941 ttgagatcgt cggtagtaaa cagcactggc aaatgctgtt ccgtcatatc ctgccagaac
    27001 ggattttccc ggttaagcac acaaggaatg cccgcctgaa tcagtaagca cagcgtacca
    27061 atgccctgct ggcgagcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga
    27121 taggcgtcaa attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc
    27181 agccccgcct gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc
    27241 accaccactt ttaccgtatc gccaaattgc tgatgaacgg cacgtaaagc ggcgacatgc
    27301 tcattgctga ggtcgccgga gttccccacc agaatggtca tttttccttc acgttgccga
    27361 tcgttcgcca tcgtattgag agaagggtcc atccgcgtcg ggaagtacag cagttctccc
    27421 cgcacctttg ggtgcgtttt ggcaaaaaag ctcaaatcgc cgcgggtggc aaatacacag
    27481 ccgactcgct tttgcgccag gcgacgtagt gggtaaaaaa gcttatatct caagccactg
    27541 gaaagctcat acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca
    27601 ccactcagca gagccagcca cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc
    27661 tgacggttag cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa
    27721 aattgcacag aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata
    27781 aactcgcgcg cgtgctcgct cgtcgcagcc agcgcgtcat tgaaaaaccg caaaacggtt
    27841 cggttatggt gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac
    27901 gccagagtaa aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg
    27961 cctgcgccgc gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata
    28021 aagtgaactg gctgatttcc gccagaatat aaaaccgcaa tgacgctttg gcgatcacca
    28081 gataaccaaa gacataagcg cccactttta acacatcacc cactaactgc caggcaaaga
    28141 gatcgcgcat agcggtaaat ttattcgaca acagcagcca gatagcaaaa tcacgcagca
    28201 gccagacggt aaaactcgcc gccgccactg ccggtaagac gaatttcagc gatttaacca
    28261 cttcccgggt gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc
    28321 tgaacgatgc cgtaataaat tgcaggtagg catcggaaat actgctcacc ccttgccaga
    28381 tccccacttc atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa
    28441 caggcaaggt caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca
    28501 gaccgttatc ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttgatcaaca
    28561 tgatagtggc aggaattacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt
    28621 cataaccgcc caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa
    28681 tcagcgataa cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt
    28741 tgccccaggc gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg
    28801 tattaccaaa caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca
    28861 gcgtagagaa accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg
    28921 gattatcatg gtactgggca acgtatttgg ttacaccgtt aaagatgcca gctccggcaa
    28981 gcacgccgag cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac
    29041 caaatgacac agccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac
    29101 tggccgccgt ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa
    29161 gtcgcaatta ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc
    29221 aggcgctcgc tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc
    29281 cccgcagggc aaccgtgcag cggaatgtaa tggaacaccg ccatgatttc cgcttctttc
    29341 agaaagttaa tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc
    29401 gcgttctgca cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga
    29461 ggcgctaacg catcgtagta gttttgccac agcgccagac gttgctggtt gatacgatcc
    29521 gctgcttcca gttgcgccca caggtacgca gcttgcagat cggacatcaa atagctggag
    29581 ccaatatcgc gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg
    29641 cccttttcac ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg
    29701 ccgccttcac cacccgccgt atagtttttg gtttcatgga agctaaagca gccaatatga
    29761 ccaatggttc ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttct
    29821 accacaaaca gattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca
    29881 cccgcgtaat ggaccggcac gataacgcgc gttttgtcgg tgatcgccgc ttcaatcagc
    29941 gtttcgtcga tgttcatggt gtccgggcga acatccacaa aaacgatttt tgcgccacgc
    30001 agcacaaagg cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca
    30061 ggctggatat cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt
    30121 aacactttgg cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg
    30181 ccatcgccac acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc
    30241 accaccggcg gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct
    30301 ttctacattc gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt
    30361 cgccacccgc aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag
    30421 ctcagcacct acaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc
    30481 ccgtaaagag acataaccgc gaataccgcc ggacgccgca cgtaaaatca gacattgatg
    30541 atcaaaggtg ccgcgcacgg cattttcaat ccactgtgca taaaagcgtc tgctggcgtc
    30601 aggcgcatac cacggcgcac gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg
    30661 taatgcggga atatcggtct cttgtgccac tacagcacca ctatcactga cattgttcac
    30721 gggtagtgcc aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc
    30781 cagttcaccc gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacac
    30841 gtctggcgtc aggagcggtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa
    30901 gaaggcgttt tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca
    30961 gcagatattg gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt
    31021 catcgagcca gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc
    31081 gtttttctac tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt
    31141 ccagccaggc aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca
    31201 tctggttgat ggaggtaatc tccagttcac cacgctccga cggcttcacc tgctttgcgt
    31261 actccacgac tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct
    31321 gttttggctt ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc accacgccaa
    31381 agcgttccgg gtccatcacc tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa
    31441 catgacgcag cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac
    31501 aagaaggttc accgttgagg aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg
    31561 ggctgggctg ttcggcgtat tccagctgga taccgaactc actaccatcg cccagcaggc
    31621 gctggaaata acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca
    31681 acatcagcac cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct
    31741 tcgatacgcc gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc aggataatac
    31801 ctttcatgtt tgcctccgct gaaaattagc ctttcagacc taaacgctcg ccctgatagc
    31861 tgccgtcctg cacctgcttc caccagcttt cattagccag ataccactgc accgttttac
    31921 gcattccact ttcaaaggtt tcctgtggca gccaaccaag ttcacgggca attttcgaag
    31981 catcaatggc ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaagtcac
    32041 gatagtgcgc cacgccgtgc ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg
    32101 tttccacaac atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga
    32161 ctttcccggt ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt
    32221 cacggatttg ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga
    32281 tcatcagcgg gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg
    32341 tgatcagcgt cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctactgg
    32401 cttttgacgc ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat
    32461 ccgtcgagtg caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg
    32521 attttttatc ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag
    32581 tcccgacaat gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc
    32641 tttcggctgc cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt
    32701 ctgcccgatc gcagatatca actttctcaa aggcaaagtg ctcgctttgc gcgaccggtg
    32761 ccagcgacat caggtttccg gcgtaggtca gcttatcgac cactaccacc gcatcgctcg
    32821 tttcgttgat gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca
    32881 gaatttttct catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt
    32941 gtcgccattg ataactttga actgactatg atcgaccagc atcaccagca cgtctgccgt
    33001 tgccagcgcc tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg
    33061 gtggatgtta ggctcaacaa ccagcgtttc gccgctgtgc cactgggcga tcagttcagc
    33121 gatttccatc gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa
    33181 gcaggcgatt ttcagttcac tggcgcgttt atcggtagca gcaaggcagt cggcaaccgc
    33241 cgctttcacc tgatcgataa cccagaacgg tttgtgatcg ttaacttcgc gcgcggtacg
    33301 gataagccgc gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca
    33361 gtgaccgccc acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag
    33421 gcgaatcagt tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc
    33481 aaaagcgata ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg
    33541 cgagttagtg acgacacact caccttcgag gaaaattttg tacagttcgc tggcgcgggt
    33601 cgaacaaacc ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac
    33661 ctggcccggt aacacgcgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac
    33721 ctgctgtggg aaagtgagat ccggacgcat ctctgctaac cattctgcca tcttctcggt
    33781 tgaccccacc ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc
    33841 aatggagcga gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg
    33901 cgtgggtaca gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa
    33961 accgccttct acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc
    34021 gccacgattg atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg
    34081 ccgtgaggca aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat
    34141 ggtcgcaaaa ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat
    34201 gcctgaccat caccatacgg gttatgggcg cggctcatag tttgatattc gttttcgtct
    34261 tttaaaagac gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc
    34321 gtacccgccg tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc
    34381 cccagcgaag gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg
    34441 ttcatcagcc agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca
    34501 tgccccagaa tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca
    34561 atctggatgt cctggtgcgt ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag
    34621 ccacgaccga aactttcacg cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg
    34681 ataaacgggt aatttgccgc cagttctgaa cgcagcgtgt cgctgctcat cacctgatca
    34741 cgcacccata acagtgcatc aatgactgta ttaccagtaa tgaagattcg gctatccgca
    34801 acgttttcac gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc
    34861 agatgcccgg tcaatgtacg gttagcctct tccggccacg gcgaatagag atcgcccgtg
    34921 cgtaaaccag cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt
    34981 gccagcgtcg ttgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga
    35041 ataggtttta gcccttccag aatccgacag gttatctctg tcaggccctg tcctggctgc
    35101 attatgttga gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg atcgagcatc
    35161 tcccgatgct gcgcagtgac gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca
    35221 tgcaccaacg gagccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc
    35281 acatcgattc tcttcgaata agcggcgagc gcctttgcgc tcgccgcagc agtgttgcta
    35341 tttcgagcaa cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca
    35401 cataatcatc aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg
    35461 caaatagcga taggtctgaa aacgcggatc cagagttgga ccaacattca gggtgtttaa
    35521 catggcccga ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc
    35581 cagtcgagcc tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc
    35641 ggcaggcaca tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg
    35701 ctcaatgctg ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac
    35761 ctgagctttc atctggatgg tgcgtgccgc ccatgcgcct ttcagctcct cattcagatg
    35821 gctggctgca cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg
    35881 cgcggtttcg gcaataagct tcacgctgtc attgaccgcg cgggtaaagt ctccggggat
    35941 aaactggatg ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac
    36001 catccgctgc ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc
    36061 cagttgcata acaaactctt tataggcttc gtccatgacc gatggttggt cggcagaagc
    36121 catgtttgaa cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag
    36181 catattcacc gttggacgat cggtaatcgc cgtcgcgctc cactcttgtc gagcaaaaaa
    36241 agtatacgcc agcgcgatta acgcaaacgc cagccccatg ccaataatcc atagcttccc
    36301 agcccacaag gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg
    36361 tttcccaggc attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca
    36421 ccacgatttc tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca
    36481 cgcttaatgc aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac
    36541 tccgggacaa aatgagaata ttctgccagc acgccaatgg aagcgagcag tgctgcggca
    36601 agggtaatca gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga
    36661 atatgctgac ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc
    36721 gccaccatat ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga
    36781 tgggttttgc cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta
    36841 ctgcccgcat cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg
    36901 atgtatggca ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg
    36961 tcgaaccaca aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag
    37021 ccatcaatgc catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc
    37081 aggaagtaac caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta
    37141 ctgagataaa gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca
    37201 cggattttta cgctgatatc aaaacggtca tccagcgcgc caatgaaaac aagcacaccg
    37261 gcacaagcga gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag
    37321 caaatccctg cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg
    37381 cggaagtttg gtttatccac taaaccgact ttttttgcca ctttacgggc aaaaaacaga
    37441 aacagtgtcg tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca
    37501 atgcatgctc tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa
    37561 gggaaagtcc gttccgaacc caccagtcct gtgcaaaaat taaccatttg atatgcaaat
    37621 tgcataacat gccagcaaat tccaggaatt ttcctaacat cgccagcgcg gcattaagat
    37681 tacagatata aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt
    37741 atgagcgttt catcatttcg aagaaatcgt cattggtctt agtcatggcc agtttattaa
    37801 tgaggaattc cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca
    37861 ttttctgcag ttcttcctga gtcgtgagca gctcttcttt acgggtaccg gaacggttgt
    37921 agtcgatagc cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt
    37981 tgcctgtacc tttaaactct tcgtaaataa cttcgtccat tttagaaccg gtatcgataa
    38041 gcgccgtcgc gataatggtc aggctgccgc cctcttccac gttacgcgcc gcaccgaaga
    38101 agcgtttcgg acgatgaagg gcattggcgt ccacaccacc ggtcaacact ttacctgacg
    38161 ccggaacaac agtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa
    38221 cgtctttctt gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa
    38281 cgtggcgaga tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct
    38341 gcatctcggt tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg
    38401 ggtggttgta agcaatgctc tgagcaatgt tctgcagcag catagtttta ccggctttcg
    38461 gcggtgccac aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc
    38521 gagcggttaa atcttcagta gaaccgttac cacgttccat acgcagacga gagtttgcgt
    38581 gcagcggggt taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt
    38641 taacttcgtt aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct
    38701 taccagagat ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt
    38761 agatgtcatc aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat
    38821 cctgcaatat ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct
    38881 tcaggatggc aaaaataatg tcctgcttac gcatacgagc caggttttcc agccccatat
    38941 tttcgccgag agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag
    39001 tggtgtgagt tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag
    39061 gatcatccat gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag
    39121 agtacgcaga actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc
    39181 gcatttaaga taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt
    39241 ttaatacgaa gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac
    39301 ggggcacccg aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg
    39361 acctttagac agtgcaccca ctttggttgc cgccacttca ccgtttttga acagcagcag
    39421 agtcgggata ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag
    39481 ttttgcaacg gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat
    39541 cattttgcac ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt
    39601 gagtacatcc gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa
    39661 ctccacagga ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa
    39721 ggttgacttt atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca
    39781 cactatgagc aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa
    39841 ggttgtagaa gcccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc
    39901 ccttccgctg acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa
    39961 aacgatggcg tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga
    40021 tcgcaaggtg aatcaaccac gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca
    40081 gatccatgcc gacgcagaac cgctggcgga agctaccggc ctgaagctgg gtctggctta
    40141 cggtggtgat ggctacgaca aacagctgaa agtgctggaa agcggcgttg acattctgat
    40201 tggcactacg gggcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat
    40261 tcaggtggtg gtactggacg aagccgatcg catgtacgat ctgggcttta ttaaagatat
    40321 ccgctggctg ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc
    40381 cacgctttcg taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat
    40441 tgaagtggaa ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc
    40501 taacgaagaa aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc
    40561 gattattttc gccaatacca aataccgttg tgaagagatc tggggccacc tggcggcaga
    40621 tggtcatcgt gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct
    40681 tgatgaattt acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg
    40741 tttgcatatt ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga
    40801 ttacgttcac cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct
    40861 ggcgtgtgaa gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat
    40921 tccggtaagc aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct
    40981 cacgcgccca cgtacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg
    41041 ttcaggttaa ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga
    41101 atgaatcccg atgagcatac tgtagtaagt gattcaggtg aaagaatgca gccaacacag
    41161 agacagattg aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc
    41221 tcggttcgaa tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc
    41281 tgacgcgaat aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt
    41341 ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag
    41401 atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg
    41461 cgggtgattt tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg
    41521 gtgaagagga agcacgtctc atttatcagg gcgttgctca cacaactggc ggtgccgatc
    41581 agcgcctggt ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac
    41641 aaaccacctc gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg
    41701 ccgatcgtaa tctggggcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt
    41761 tacgtccggt tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg
    41821 gcaccgtgca ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc
    41881 tggaaaagtt gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg
    41941 agattgacgg gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga
    42001 tcgccatttt taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg
    42061 aaggcctggt ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc
    42121 tgcgtaatat tcagcgccgc tttatgatcg atattgatca ggcgcagcgc gtagccaaag
    42181 ttgcggctaa cttcttcgat caggtggaaa aggaatggca tcttgaagca ataagccgcg
    42241 atttgctcat cagcgcctgt cagcttcatg aaatcggcct gagcgttgac tttaaacaag
    42301 cgccgcaaca cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac
    42361 agaaaaaatt gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc
    42421 tgcatcagca aaatgccgta cctccgcgcg tcgcagaaca actctgccgt ttactccgcc
    42481 tggccatcat ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg
    42541 ctaaccatga actgttgacc ttgacgcttc cgcaaggttg gctaactcaa catccgctgg
    42601 gtaaagagat tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag
    42661 tgcattaatg agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt
    42721 ccctcgtttt gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat
    42781 ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag
    42841 ctccagcaaa aagcggctcg gctccgggcg caccagctcg ccgtactgac ggcgttcttt
    42901 acacagcgta aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg
    42961 ctcctcatcg atattatctt catcgatgct gctctggtgc ggcaaaaacc cttcttccat
    43021 accgaccatg tagacataag gaaactccag cccttttgac gcgtgcagag tcatcagttg
    43081 cacctgatcc agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg
    43141 cgtcaccacc tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt
    43201 catccagcta aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg
    43261 cgatgtttcg tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc
    43321 aatcggctcc cgctccgcca gacgctggat ttctgccaac cagtgagtaa agcgggtcaa
    43381 tgcttcataa ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa
    43441 catgctttta ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat
    43501 ctctctcttc ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag
    43561 cacgcgcaga taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc
    43621 agaaattttg tacgggatgc ggttttgcat caggaatttt tcaaacactc gcgactgatg
    43681 gttaccgcga taaagaatag cgtaatcttt gtactgcgtt ttattgacga agtgatgcgc
    43741 aatcagttca ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt
    43801 aagctctgtg ccgtaaccca gttcggagaa cagacgcttc tcaaagacgt gcgggttatt
    43861 ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag
    43921 cttaatcacc ttcagcgccg gaaaatcctg actcagcagc accaggtttt gtggacgtgc
    43981 gccgcgccag gagtagatcg actggtcgtc atcgcccacc acggtaaagc gcgcgcggct
    44041 gcccaccagc agtttcacca gttcatactg gctggtgttg gtgtcctgat actcatccac
    44101 cagcagatag cgaattttgt tctgccagcg ctcgcggact tcttcattgc gttgcagcag
    44161 caacgtcggc aataaaatca gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc
    44221 atcatacagc ccataacaat gggcaaaaat acgatcccgc tcgccctttg cctctgccgc
    44281 cgcctgcgcc ggagttttga gatcgttctt ccagttggag atggtcgaaa tcagctgttg
    44341 caggagaact ttgtcatctt caatcagccc ctcggtcaac tctttaagca aagcaagctg
    44401 atcggtatcg tcaaacagcg aaaagttcgc cttcatccca agcgccgcat actcgcgttt
    44461 gatgatatcc aaccccagcg tgtgaaaagt ggagatcatc aacccgcgcg cctctttgcg
    44521 acccagcgtc tgcccgacac gctctttcat ctcgcgcgcc gccttattgg taaaggtcac
    44581 cgccgcgata tgtcgcgcct gataaccgca accgcggatc agatgggcga ttttattggt
    44641 gatcacacga gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa
    44701 ttcgacagct tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac
    44761 ggggggagga ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt
    44821 tggctgcgga tgtttaaatc agagagagaa gaccgaaatc ggagttcatc gtgaatccat
    44881 tagacttaga aaatatcggg tcgtaacgtt gtgttattac tttttcagag tttactaagg
    44941 agaggtttac tcgtggcgat taatttcagc cctaaggttg gtgaaataca ggaatgcaac
    45001 tttggtaact atcctgttat ccagaatgga cagttcagca ccacgtatta cgatggccgc
    45061 attcctcctg aaatgataaa aaacaggtta gttgtcgtgc tcaacggaaa aattaatggc
    45121 aacgcatgta tcgtcgttcc attatcaacc actcgcgatc atgacaagtt aaatcgtgga
    45181 atgcatgttg aaatagccag taatgttatc aatggcctgc aattctttga tcagcagatt
    45241 cgttgggcca aagccgacct ggtacaacaa gtcagtcgaa accgattaaa tagagcaagg
    45301 acttacagag ggtatcttaa tcaatgtttg ccacatgagt tggttgcaga catacagagg
    45361 gcggtcatca agtcaatcaa tgccatatcg ctcataaatt gacaataaaa taaccatccc
    45421 cgataatgac tttgtagccc atagaggccc taagttttct ccccttgagg gacctgcgaa
    45481 gcccggattt tccgggcttt tgccatatat gtaccagccc ctccctgact gacaattacg
    45541 ttagcatctg cggatcatgc taaaatcgcc gcctgacaat tattcactca aggcacgatc
    45601 atggcaaaaa cagcagcagc actgcatatc cttgtaaaag aagagaaact ggctctggat
    45661 cttctcgagc agattaagaa cggtgccgat ttcggcaagc tggcgaagaa acactctatt
    45721 tgcccgtcag gcaaacgcgg cggtgattta ggtgaattcc gccagggtca gatggttccg
    45781 gcgttcgata aagtggtttt ctcttgtccg gtactggagc cgaccggccc gctgcacacc
    45841 cagttcggat atcacatcat taaggtgctg taccgcaact aatagcaagg ccttctccag
    45901 gagaaggcct tgagtgtttt ctccctctcc ctgtgggaga gggtcggggt gagggcatca
    45961 gcgcgcactt aacccgcaac agcaatacgt ttcatatctg tcatatagcc gcgcagtttc
    46021 ttacctacct gctcaatcgc atggctgcga atcgcttcgt tcacatcacg cagttgcgcg
    46081 ttatctaccg cgccttccgg aatagcttta cccaagtcgc ccggttgcag ctctgccata
    46141 aacggtttca gcaacggcac acaagcgtaa gagaacagat agttaccgta ctcagcggta
    46201 tcagagataa ccacgttcat ttcgtacaga cgcttacggg cgatggtgtt ggcaatcagc
    46261 ggcagctcgt gcagtgattc ataatatgca gactcttcaa tgatgccgga atcgaccatg
    46321 gtttcgaacg ccagttcaac gcccgctttt accatcgcga tcatcagtac gcctttatcg
    46381 aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcaaa cgcggttttg
    46441 ccggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc cgccatcata
    46501 ccggaagaga attcgccgga gatgatgtcg tccatatgtt tctggaacag cggtgccatg
    46561 atctctttca gctgttctga cagcgcataa gcacgcagtt tcgccgggtt agagagacgg
    46621 tccatcatca gggtgatgcc gccctgcttc agcgcttcgg tgatggtttc ccaaccgaac
    46681 tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt gtcgaagcac
    46741 agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat caggtcagat
    46801 ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc ggttgctgcc
    46861 gcccatgctt tagcgatcgc cataccttcg cctttcggat cgttttccgg gtgaacggca
    46921 atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt gccagggcat
    46981 ttcggcgcaa ccatgactac ggtgatatct ttacggatct gctcgcccac ttcgacgatg
    47041 ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg tacggtgcgc
    47101 actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc ctgcgggatc
    47161 agttcttcgt aagtaccaac tttaaaaccg ttttcggtcg ctttacgcca ggacgcgcgc
    47221 ttctcggcaa tcgcttcttt acgcagagcg taggagatat ccagaccaga atcacgcatg
    47281 ttcaggccct ggttcagacc ctgtgcgcca cagccgacga tgactacttt tttaccctga
    47341 aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt gcccagctgt
    47401 gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat tcctcgtgat
    47461 gttgtgcttc ttattgttcg gttcgcgttt gcgagattga attcactata tgacaggaaa
    47521 tttattgcgg aaattgatat attcacaacg tcacattgca atttttgcaa cgtcaacatc
    47581 gagggctgcg ccattggatt tacgcgatct gaaaaccttc ctgcatctgg cggaaagccg
    47641 ccattttggc cgcagcgcgc gggcgatgca cgttagccca tccacgctct cacggcagat
    47701 tcagcgcctg gaagaagatc ttggtcagcc gctatttgtg cgcgataacc gcacggtaac
    47761 gctgacggaa gcgggcgaag agctgcgcgt tttcgcccag caaacgctgt tgcagtatca
    47821 gcaattgcgc cacaccatcg atcagcaagg gccgtcgctc tctggcgaat tacatatctt
    47881 ctgctcggtg accgctgcct acagccatct gccgccgatt ctggatcgct tccgcgcgga
    47941 acacccgtcg gtggagatca aactcaccac tggtgatgcg gcagatgcaa tggaaaaagt
    48001 ggtcacggga gaagcggatc tggcgattgc cgggaaaccg gagacgctac ccggcgctgt
    48061 ggcgttttcg atgctggaga atctggcagt agtgctgatt gcgcccgcgc tgccctgccc
    48121 ggtgcgtaac caggtttcgg cagagaagcc ggactggtct acagtgccgt ttatcatggc
    48181 cgatcaggga ccggtgcgtc gccgcattga actgtggttt cgacgcaata aaatcagcaa
    48241 cccgatgatt tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg tggcgctcgg
    48301 ctgtggtgtg gcgctgttgc cggaagtagt gctggaaaac agccccgaac cggtgcgtaa
    48361 ccgcgtgatg attttagagc gcagcgatga aaaaacgccg tttgaactgg gcgtgtgtgc
    48421 acaaaaaaag cggctgcatg agccgctgat tgaggcgttc tggaggattt tgccgaacca
    48481 caaatgacgt tgtcgcgcgg gtaggcctga taagcgaagc gctatcaggc atttttccct
    48541 aacccgccaa aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca
    48601 gctcattcag ccgggtttcg aaatccggtt catggtcgcc aagttcgaac gccgccagta
    48661 cgcgcccgta gtcggtgcca tggctgcgat agtggaacaa agaaatgttc cagtgcgtac
    48721 ccagcgtgtt gaggaagcgc agcagcgcgc ccggtgattc cgggaattcg aagctgtaga
    48781 ggcgttcctg caacggatgt gacggacgcc cgccgaccat atagcgcaca tgcaacttcg
    48841 ccatttcgtc gtcggagaga tccaccacgc tgtagccgcc gtcgttgagc atctgcaaaa
    48901 tttctttgcg ctcttcgagg ccgcggctca ggcgcacgcc gacaaagatg caggcgtttt
    48961 tggcatcggc aaaacggtag ttgaactcgg tgaccgatcg cccgccaagc agttggcaga
    49021 atttgaggaa gctgcctttt tcttccggaa tggtcaccgc caacaacgct tcacgctgtt
    49081 cgcccagttc gcagcgttcc gagacgtagc gcaggccgtg gaagttcacg ttggcaccgg
    49141 aaagaatatg cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc
    49201 ccgccagcgc cagcgcgcca gagggttccg ccaccgcgcg cacatcttcg aataaatcct
    49261 tcatcgccgc acagatcgca tcactatcga cggtgatgat gtcgtcgaga tactcctggc
    49321 ataaacggaa ggtttcgtca ccgatgcgtt ttaccgctac gccttcagca aatagcccta
    49381 cgcgcggcag atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt
    49441 ccgcttctac ggcgatcact ttgatttgtg gcatcagttg tttgatcagc accgcaacgc
    49501 ccgccgccag accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct
    49561 ggagcagttc cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg
    49621 gcacccaggt gaacccctgc tgctgtgaca gttcgatcgc tttagctttc gcttcgtcaa
    49681 agttcgcgcc atgaagcagc acttcgccgc cgaatccacg taccgcatcg actttgatgt
    49741 cggcggtggc ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg
    49801 cgacgccctg cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt
    49861 ccgtcaggcc cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc actggctggc
    49921 gatcttcgcg cttcaccaga atgacgttat caagacgcga cgacagtttt tccatttttt
    49981 gtagcggcgt aacctgcgcc gcctcgtaaa ccggcgcgcg cagcactgct cttaaatatt
    50041 cggcaccttc cggagcaccg gacaggggtt gcgagtcagc cattattaac cccccagttt
    50101 cgatttatcg cgcaccgcac ctttgtcggc gctggttgcc aggctggcgt aggcgcgcag
    50161 ggcaaaggaa acctgacgtt cacggttttt cggcgtccag gctttgtcac ctcgagcgtc
    50221 ctgcgcttca cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt
    50281 cgggatgtct atagcgatca ggtcaccatc ttcaatcagg ccaatgctgc cgccgcttgc
    50341 cgcttccggt gagacgtggc cgatggaaag accagaggtg ccgccagaga aacgaccgtc
    50401 ggtgatcagc gcacaggctt taccgagacc cattgatttc aggaagctgg ttgggtagag
    50461 catttcctgc atgccagggc cgcctttcgg gccttcatag cggatcacca ccacatcgcc
    50521 cgcgacaact ttgccaccga ggatcgcttc taccgcgtcg tcctggcttt cgtacacttt
    50581 cgccgggccg gtgaatttga ggatgctgtc atcgacgccc gcggttttaa cgatgcagcc
    50641 gttttctgcg aaattaccgt agagcaccgc caggccgccg tctttgctgt aggcgtgttc
    50701 cagcgagcgg atacagccat tggcgcgatc gtcatcgaga gaatcccaac ggcaatcctg
    50761 cgagaatgcc tgtgtggtac gaatgcctgc aggacctgcg cggaacatat tttttaccgc
    50821 gtcatcctgg gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg tcaggccaag
    50881 tacgtttttc acatcacggt tcagtaaccc cgcgcgatcc agttcgccga gaataccgat
    50941 aacaccacca gcacggtgaa catcttccat atggtatttc tgggtgctcg gcgcaacttt
    51001 acacagctgt ggaaccttgc gggaaagctt atcgatatca ctcatggtga agtcgatttc
    51061 cgcttcctgc gccgccgcca gcaggtgaag tacggtgtta gtcgatccac ccatcgcgat
    51121 atccagcgtc atggcgtttt caaacgccgc tttgctagca atattgcgcg gcagtgcact
    51181 ttcgtcgttt tgctcgtagt aacgtttggt caattcaaca atgcgtttac cggcattgag
    51241 gaacaactgc ttacggtcgg cgtgggttgc cagcagcgag ccgttgcccg gctgcgacag
    51301 acccagcgct tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggagcc
    51361 gcaggtcgga cacgcggaac gttcaacctg atcgctctgg gagtcagaaa ctttcgggtc
    51421 tgcgccctgg atcatcgcat caaccagatc gagtttgatt atctgatcgg acagtttggt
    51481 tttcccggcc tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcaggga
    51541 agccatcagc atccccgggg tgattttgtc gcagttagag atgcagacca tggcgtcggc
    51601 gcagtgggcg ttgaccatat actcaacgga atcagcgatc agttcgcgag atggcagtga
    51661 ataaagcatc cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc
    51721 tttggcaacg ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag
    51781 atggacgtga cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttgcc
    51841 aaaatcggcg tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg
    51901 accatgagtg gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt
    51961 ctcgtaaatg ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta
    52021 accagcccca tttatcttcg gtttcgccag tgaagaggcc gaagaaggct tgctgaatgc
    52081 gtttggtaac cgggccacaa cggccttcgc caacctgaat accatctaca ctgcgcactg
    52141 gcgtgatttc tgccgcagta ccggacataa acacttcatc cgccaggtac agggattcgc
    52201 gcgacagaac ctgctcacgc acttcaattc ccagctcttt cgccagtttg atgatggcat
    52261 cacgggtaat acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt
    52321 tcacttcaaa caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga
    52381 taccttcctg ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagagatagt
    52441 taccaccggc ttttgccgcg gttgggatgg tgtttggtgc tgcgcggttc caggaggaaa
    52501 ccatcgcatc gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag
    52561 cggcgataat cacatcggtt gagtatcccg ctggcgggtt tacacccatg ccaacatcac
    52621 caacgaagat cagcggacgg atataggcgc tggtgagatt gtttttgcgg atcacgtcac
    52681 gacaagcttc catcagctca tcaatgctct gcgaaaccgg gaagcgatag attttggcgg
    52741 agtcatgcag acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgcgagt
    52801 cgtagcaacg gatgccttca aaaaccgaag tgccatagtg cagcgcgtgc gacatcacat
    52861 gcaccttcgc gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct
    52921 tcgtggtcat ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg
    52981 ctctggcaga tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag
    53041 tcgaccgacc gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg
    53101 gcagccatat tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt
    53161 tctaaggttt ccggattgaa gcgagccgat acattgacct gatgttgcat catgataatt
    53221 tctccaacat ttctgaatta ctggcgccag gcggcaccag cggccagacg ttctcaagtt
    53281 cgtcgattga gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg
    53341 cttcaacctg gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca
    53401 tgaggaaatc ggggttatca gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt
    53461 gctgccattg tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta
    53521 actgcttgcg ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg
    53581 agatacagac gacagtatcg ttcggtcgtg cgacttgtgc gccaaccgcc gctggtaaac
    53641 cgaaacccat ggtgcctaag ccgctggagg taatgaaatt ttccgggcga gtgtgtgcga
    53701 tgtgctgcgc ggcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag
    53761 gtttacgatc cgacagttgt tttaacaaca acggcgcgta gatagcgtca ccgggatggt
    53821 cgtaacgcca ggtatgttca tcacgcagct gcgcgcagta ttgctgccag tcattgatat
    53881 ttaacggctg ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg
    53941 cctgacgcag cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg
    54001 gtgcgaaggt gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca
    54061 gcaagtcgca ctcctgcacc gcgaagttcg ccgctttggt gccatgcatt cccagcatgc
    54121 ccaggtagta cggataatct gcttcaactg cgcccagccc tttcagcgtg caggtggcag
    54181 gcatttttgt ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc atacccacgc
    54241 caccaccaac gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcaa
    54301 cttcggcatg tgggaaagtc acttcgtttt caacggtggt gaaccacggt tccaggtcac
    54361 cgctggctag ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc
    54421 tggcaacgtc gaatgcttca gccataatgc gcggcaactc ttccagcgac tgcaccagaa
    54481 agctgtgctt ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtccg
    54541 tgccgataaa cggtgcggac acttgaccgg tgatggcaac aacagggata gaatctaaca
    54601 gtgcgtccgc aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata
    54661 cgccagtttt gccggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat
    54721 gtcggcacag caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg
    54781 cgccacccgg ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc
    54841 actgtgcgcc attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct
    54901 gttattcatt gtctgctcct cggttatatt tttaaggtca aaaaaaaccc ccggaccttt
    54961 cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg tccaagtgca
    55021 gccccgcacg gtgggataat aatcaccacc acgctaatca tgaccaggct aatcactcgt
    55081 agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac
    55141 catagatttt gcgaatagca caagatattt tttggccgat ttttatgatg cgtttaa
//
LOCUS       DAZFKM010000017        54544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFKM010000017 DAZFKM010000000
VERSION     DAZFKM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            288..374
                     /gene="leuL"
                     /locus_tag="ACHKAY_001122"
     CDS             288..374
                     /gene="leuL"
                     /locus_tag="ACHKAY_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO5699831.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            467..2038
                     /gene="leuA"
                     /locus_tag="ACHKAY_001123"
     CDS             467..2038
                     /gene="leuA"
                     /locus_tag="ACHKAY_001123"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO5699832.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2038..3129
                     /gene="leuB"
                     /locus_tag="ACHKAY_001124"
     CDS             2038..3129
                     /gene="leuB"
                     /locus_tag="ACHKAY_001124"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO5699833.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3132..4532
                     /gene="leuC"
                     /locus_tag="ACHKAY_001125"
     CDS             3132..4532
                     /gene="leuC"
                     /locus_tag="ACHKAY_001125"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO5699834.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4543..5148
                     /gene="leuD"
                     /locus_tag="ACHKAY_001126"
     CDS             4543..5148
                     /gene="leuD"
                     /locus_tag="ACHKAY_001126"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO5699835.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5197..6375)
                     /gene="setA"
                     /locus_tag="ACHKAY_001127"
     CDS             complement(5197..6375)
                     /gene="setA"
                     /locus_tag="ACHKAY_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO5699836.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6477..6608)
                     /gene="sgrT"
                     /locus_tag="ACHKAY_001128"
     CDS             complement(6477..6608)
                     /gene="sgrT"
                     /locus_tag="ACHKAY_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO5699837.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6697..8352
                     /gene="sgrR"
                     /locus_tag="ACHKAY_001129"
     CDS             6697..8352
                     /gene="sgrR"
                     /locus_tag="ACHKAY_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO5699838.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8386..8485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8516..9499
                     /gene="thiB"
                     /locus_tag="ACHKAY_001130"
     CDS             8516..9499
                     /gene="thiB"
                     /locus_tag="ACHKAY_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO5699839.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDLRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9475..11085
                     /gene="thiP"
                     /locus_tag="ACHKAY_001131"
     CDS             9475..11085
                     /gene="thiP"
                     /locus_tag="ACHKAY_001131"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO5699840.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11069..11767
                     /gene="thiQ"
                     /locus_tag="ACHKAY_001132"
     CDS             11069..11767
                     /gene="thiQ"
                     /locus_tag="ACHKAY_001132"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO5699841.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTMPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11881..12645)
                     /gene="yabI"
                     /locus_tag="ACHKAY_001133"
     CDS             complement(11881..12645)
                     /gene="yabI"
                     /locus_tag="ACHKAY_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5699842.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12731..13609)
                     /gene="araC"
                     /locus_tag="ACHKAY_001134"
     CDS             complement(12731..13609)
                     /gene="araC"
                     /locus_tag="ACHKAY_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO5699843.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13948..15648
                     /gene="araB"
                     /locus_tag="ACHKAY_001135"
     CDS             13948..15648
                     /gene="araB"
                     /locus_tag="ACHKAY_001135"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO5699844.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15659..17161
                     /gene="araA"
                     /locus_tag="ACHKAY_001136"
     CDS             15659..17161
                     /gene="araA"
                     /locus_tag="ACHKAY_001136"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO5699845.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17330..18025
                     /gene="araD"
                     /locus_tag="ACHKAY_001137"
     CDS             17330..18025
                     /gene="araD"
                     /locus_tag="ACHKAY_001137"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5699846.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18100..20451
                     /gene="polB"
                     /locus_tag="ACHKAY_001138"
     CDS             18100..20451
                     /gene="polB"
                     /locus_tag="ACHKAY_001138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO5699847.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20616..23522
                     /gene="rapA"
                     /locus_tag="ACHKAY_001139"
     CDS             20616..23522
                     /gene="rapA"
                     /locus_tag="ACHKAY_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO5699848.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23534..24193
                     /gene="rluA"
                     /locus_tag="ACHKAY_001140"
     CDS             23534..24193
                     /gene="rluA"
                     /locus_tag="ACHKAY_001140"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO5699849.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24310..25125)
                     /gene="djlA"
                     /locus_tag="ACHKAY_001141"
     CDS             complement(24310..25125)
                     /gene="djlA"
                     /locus_tag="ACHKAY_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO5699850.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25380..27734
                     /gene="lptD"
                     /locus_tag="ACHKAY_001142"
     CDS             25380..27734
                     /gene="lptD"
                     /locus_tag="ACHKAY_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO5699851.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEPPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKHYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27787..29073
                     /gene="surA"
                     /locus_tag="ACHKAY_001143"
     CDS             27787..29073
                     /gene="surA"
                     /locus_tag="ACHKAY_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO5699852.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29073..30062
                     /gene="pdxA"
                     /locus_tag="ACHKAY_001144"
     CDS             29073..30062
                     /gene="pdxA"
                     /locus_tag="ACHKAY_001144"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO5699853.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30059..30880
                     /gene="rsmA"
                     /locus_tag="ACHKAY_001145"
     CDS             30059..30880
                     /gene="rsmA"
                     /locus_tag="ACHKAY_001145"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO5699854.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30883..31260
                     /gene="apaG"
                     /locus_tag="ACHKAY_001146"
     CDS             30883..31260
                     /gene="apaG"
                     /locus_tag="ACHKAY_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO5699855.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31267..32115
                     /gene="apaH"
                     /locus_tag="ACHKAY_001147"
     CDS             31267..32115
                     /gene="apaH"
                     /locus_tag="ACHKAY_001147"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO5699856.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32140..32739
                     /locus_tag="ACHKAY_001148"
     CDS             32140..32739
                     /locus_tag="ACHKAY_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO5699857.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32775..33242
                     /locus_tag="ACHKAY_001149"
     CDS             32775..33242
                     /locus_tag="ACHKAY_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5699858.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33341..33520)
                     /locus_tag="ACHKAY_001150"
     CDS             complement(33341..33520)
                     /locus_tag="ACHKAY_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO5699859.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33541..34020)
                     /gene="folA"
                     /locus_tag="ACHKAY_001151"
     CDS             complement(33541..34020)
                     /gene="folA"
                     /locus_tag="ACHKAY_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO5699860.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34212..36074)
                     /gene="kefC"
                     /locus_tag="ACHKAY_001152"
     CDS             complement(34212..36074)
                     /gene="kefC"
                     /locus_tag="ACHKAY_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO5699861.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36067..36597)
                     /gene="kefF"
                     /locus_tag="ACHKAY_001153"
     CDS             complement(36067..36597)
                     /gene="kefF"
                     /locus_tag="ACHKAY_001153"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO5699862.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36705..38036)
                     /gene="yaaU"
                     /locus_tag="ACHKAY_001154"
     CDS             complement(36705..38036)
                     /gene="yaaU"
                     /locus_tag="ACHKAY_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5699863.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38095..38382)
                     /gene="fixX"
                     /locus_tag="ACHKAY_001155"
     CDS             complement(38095..38382)
                     /gene="fixX"
                     /locus_tag="ACHKAY_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO5699864.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38379..39665)
                     /gene="fixC"
                     /locus_tag="ACHKAY_001156"
     CDS             complement(38379..39665)
                     /gene="fixC"
                     /locus_tag="ACHKAY_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5699865.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39716..40657)
                     /gene="fixB"
                     /locus_tag="ACHKAY_001157"
     CDS             complement(39716..40657)
                     /gene="fixB"
                     /locus_tag="ACHKAY_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5699866.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40672..41442)
                     /gene="fixA"
                     /locus_tag="ACHKAY_001158"
     CDS             complement(40672..41442)
                     /gene="fixA"
                     /locus_tag="ACHKAY_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO5699867.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41916..43430
                     /gene="caiT"
                     /locus_tag="ACHKAY_001159"
     CDS             41916..43430
                     /gene="caiT"
                     /locus_tag="ACHKAY_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO5699868.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43461..44603
                     /gene="caiA"
                     /locus_tag="ACHKAY_001160"
     CDS             43461..44603
                     /gene="caiA"
                     /locus_tag="ACHKAY_001160"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO5699869.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44732..45949
                     /gene="caiB"
                     /locus_tag="ACHKAY_001161"
     CDS             44732..45949
                     /gene="caiB"
                     /locus_tag="ACHKAY_001161"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO5699870.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46023..47576
                     /gene="caiC"
                     /locus_tag="ACHKAY_001162"
     CDS             46023..47576
                     /gene="caiC"
                     /locus_tag="ACHKAY_001162"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO5699871.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47685..48470
                     /gene="caiD"
                     /locus_tag="ACHKAY_001163"
     CDS             47685..48470
                     /gene="caiD"
                     /locus_tag="ACHKAY_001163"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO5699872.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48476..49066
                     /gene="caiE"
                     /locus_tag="ACHKAY_001164"
     CDS             48476..49066
                     /gene="caiE"
                     /locus_tag="ACHKAY_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO5699873.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49152..49547)
                     /gene="caiF"
                     /locus_tag="ACHKAY_001165"
     CDS             complement(49152..49547)
                     /gene="caiF"
                     /locus_tag="ACHKAY_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO5699874.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49808..53029)
                     /gene="carB"
                     /locus_tag="ACHKAY_001166"
     CDS             complement(49808..53029)
                     /gene="carB"
                     /locus_tag="ACHKAY_001166"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO5699875.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53047..54195)
                     /gene="carA"
                     /locus_tag="ACHKAY_001167"
     CDS             complement(53047..54195)
                     /gene="carA"
                     /locus_tag="ACHKAY_001167"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO5699876.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13154 a        14751 c        14259 g        12380 t
ORIGIN      
        1 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
       61 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
      121 agttaagcag aaattaatat cgcttacttt aaccaccgca gcacaattag ctaattttac
      181 gggtgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
      241 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
      301 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
      361 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
      421 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
      481 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
      541 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
      601 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
      661 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
      721 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
      781 gcacatcgcc accaagctgc gcagcaccct ggacgaggtg atcgaacgcg ctatctatat
      841 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
      901 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
      961 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     1021 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     1081 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     1141 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     1201 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccagga
     1261 aatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     1321 agccattgtt ggcagcggcg cattcgcaca ctcctccggt atccaccagg atggcgtgct
     1381 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     1441 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg acgagatggg
     1501 gtataaagaa agtgaatata atttagacaa tttgtacgac gccttcctga agctggcgga
     1561 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     1621 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     1681 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     1741 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     1801 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     1861 tattgtcgcc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     1921 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     1981 cgaaaaagag ttgcaacgca aagctcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     2041 tcgaagaatt accatattgc cgtattgccg ggggacggta ttggtccgga agtgatgacc
     2101 caggcgctga aagtgctgga tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     2161 cattacgatg taggcggtgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     2221 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg tccgaaatgg
     2281 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgttgccttt gcgtaagcac
     2341 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     2401 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc tgtgcgtgcg cgaactgacc
     2461 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     2521 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     2581 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     2641 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     2701 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     2761 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     2821 ggctcgatgg ggatgttgcc ttccgccagc ctgaacgagc aaggttttgg tctgtatgaa
     2881 ccggcaggcg gctcagcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcgcaa
     2941 attctgtcgc tggcactgct gctgcgctac agcctggatg ccgatgatgc ggcttgcgcc
     3001 attgaacgcg ccattaaccg cgcattagaa gaaggcattc gcaccgggga tttagcccgt
     3061 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     3121 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     3181 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     3241 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     3301 ccttcgccac tatggatcac aacgtctcta cccagacgaa agacattaat gcctgtggtg
     3361 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgaac
     3421 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     3481 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     3541 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacgcaaa
     3601 ccctgaaaca gggccgcgca aaaaccatga aaattgaagt ccagggcaaa gccgcgccgg
     3661 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aaccggtagc gcaggcggca
     3721 ccgggcatgt ggtggagttt tgcggcgaag caatccgtga tttaagcatg gaaggtcgta
     3781 tgaccctgtg caatatggca atcgaaatgg gcgcaaaagc cggtctggtt gcaccggacg
     3841 aaaccacctt taactatgtc aaaggccgtt tgcatgcgcc gaaaggcaaa gatttcgacg
     3901 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     3961 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat ccaggccagg
     4021 tgatttccgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgcg
     4081 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggcattccg ctgaccgaag
     4141 tggctatcga caaagtgttt atcggttcct gtaccaactc gcgcattgaa gatttacgcg
     4201 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     4261 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atctttattg
     4321 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     4381 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     4441 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gcagtgaccg
     4501 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     4561 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     4621 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     4681 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     4741 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     4801 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     4861 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
     4921 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
     4981 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
     5041 ttccgccgcc actgcatgat gaacggtctg gacagtattg ggcttacctt gcagcacgac
     5101 gacgccattg ccgcttatga agcaaaacaa cctgcgttta tgaattaatc cccttgcccg
     5161 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
     5221 taaaaataat gcgacaacag aaataaccgc aattacccag tagacagcaa agtgccccca
     5281 actttgtgca attgctccct gaataacgcc agccagaatt accccggtag aaatactgtt
     5341 agtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
     5401 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
     5461 catacggcta tgaaagaaaa tcaatccggt gtaaaacagt actcctgccg ccactgctat
     5521 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
     5581 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
     5641 ctcgctactg atccacaacg gcatatcaat aatgtacatg gtgttgcagg tccacattaa
     5701 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagccacctt gcattgataa
     5761 agcattttcc gacggcagtt ctacccgcgc cacagacgga agcataaatg caatcaatac
     5821 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc accgtaaagc cgtaattcaa
     5881 cgccagcata aaggccaacg gtggaccgat aacccatgcc agagaaagct gcgcacgcat
     5941 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcatattccc gcgccagagc
     6001 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
     6061 cgtcagataa tgacgattaa atgcaaacaa tagcgcattg ccgatagcca tcaaacagca
     6121 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
     6181 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
     6241 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
     6301 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
     6361 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
     6421 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
     6481 ctttcagaat tgcggtcatc ccactgcatc agttcttcaa gcatggttaa tcgttgtggg
     6541 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
     6601 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
     6661 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
     6721 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
     6781 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
     6841 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
     6901 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
     6961 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
     7021 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
     7081 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
     7141 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
     7201 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
     7261 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
     7321 aatacgctgc cgctctattc gcatattgcg gacattgtgt cgccgacgcc ctggacgctg
     7381 gatatccatc tcacgcaacc ggaccgctgg ttaccgttac tgctgggaca agttccggcg
     7441 atgatcctgc cgcgcgaatg ggaaaccctc agtaactttg ccagccatcc catcggcacc
     7501 ggtccgtatg cggtgatccg caacaccacc aatcaactga aaattcaggc attcgatgac
     7561 ttcttcggtt accgggcatt aatcgatgaa gttaacgtct gggttctgcc ggaaattgcc
     7621 gacgagccag ctggaggtct gatgctaaaa ggtccacagg gtgaggaaaa agagattgaa
     7681 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
     7741 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
     7801 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
     7861 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcat cctgaccttt
     7921 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
     7981 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
     8041 gagagcgata tctggcttaa cagcgccaac tttacgctgc ctctggattt ttcgctgttc
     8101 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
     8161 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
     8221 agcaaagcaa tggtgccgct tatccaccac tggctgatca ttcaggggca acgcagtatg
     8281 cgcggcctgc gcatgaacac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
     8341 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
     8401 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
     8461 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
     8521 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
     8581 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
     8641 agcttttgaa gccgactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
     8701 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
     8761 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
     8821 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
     8881 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
     8941 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatctgc gcaccagtac
     9001 gccggggctg ggcctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
     9061 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
     9121 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
     9181 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
     9241 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
     9301 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
     9361 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
     9421 gctggagttc acaccagctg aagtggctgc acaacgtcag gcatggatta gcgaatggca
     9481 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
     9541 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
     9601 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
     9661 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
     9721 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
     9781 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
     9841 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
     9901 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
     9961 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    10021 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    10081 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    10141 gcaactacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    10201 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    10261 cgactaagta aggccattgc gcccggtacc acgctgctgc aaggctggcg cgatccggac
    10321 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    10381 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    10441 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    10501 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    10561 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    10621 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    10681 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    10741 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    10801 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    10861 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    10921 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    10981 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    11041 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    11101 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    11161 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    11221 agccagcggt tcgctgacta tcgacagcgt tgatcacacc acgatgccgc cgtcacgccg
    11281 cccggtatcg atgctgtttc aggagaacaa cctgttcagc cacctgacgg tcgcgcagaa
    11341 catcgggctg gggcttaatc cgggattgaa gctgaacgcg gcgcagcagg agaaaatgca
    11401 cgctatcgcc cgccagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    11461 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    11521 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    11581 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    11641 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    11701 gggtaagacc aatgagttgt tgagcggtaa ggcgagtgct tcggcactat tggggattac
    11761 gggttagtat gcactgtcgg atgcggcgtg aacgccttat ccgacctacg ggtagtgcgt
    11821 tttgtaggcc tgataagacg cggtcagcgt cgcatcaggc aacccgcaca agactatctc
    11881 ctaaccccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    11941 taacaccacc agcgccacca cgccgatggc agaaattaac ggcgtcagcc acaacaaacg
    12001 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    12061 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    12121 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    12181 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    12241 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    12301 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    12361 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    12421 gaaggagatc cagtcgccca gcaggcaacc gacgatccct gccagccagg cgtgccagaa
    12481 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    12541 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    12601 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    12661 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    12721 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggctc
    12781 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    12841 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    12901 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    12961 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    13021 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
    13081 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    13141 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    13201 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
    13261 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    13321 aaacccactg gtgataccat tcgcgagcct ccggataacg accgtagtga tgaatctctc
    13381 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
    13441 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    13501 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    13561 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    13621 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    13681 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    13741 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    13801 gaaaagtcca cattggttat ttgcacggcg tcacactttg ctatgccata gcatttttat
    13861 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    13921 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    13981 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    14041 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    14101 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    14161 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    14221 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    14281 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    14341 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctacattgg cggtatttat
    14401 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacac cgccgtggcg
    14461 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    14521 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataagtc gctatggcac
    14581 gaaagctggg gtggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    14641 cgccatttac cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    14701 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    14761 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    14821 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    14881 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tacctggatt tatcggtctg
    14941 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    15001 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    15061 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    15121 ccggtggtgc tcgactggtt taacggtcgt cgcacaccga acgctaacca acgcctgaaa
    15181 ggggtgatta ccgatcttaa cctcgctacc gacgctccgc tgctgttcgg cggtttgatt
    15241 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    15301 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    15361 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    15421 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    15481 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    15541 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    15601 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    15661 gacgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    15721 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    15781 agcgaaactg ccctgcaaac tagtgttgaa accgctgggc accacgccgg atgaaatcac
    15841 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    15901 cactttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    15961 gcaattccac acccagttta acgcggcgct gccgtgggac agtatcgata tggactttat
    16021 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    16081 tcagcaacat gccgtggtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    16141 ctcctggatg cgccaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgttt
    16201 tggcgataac atgcgtgaag tagcggtcac cgatggtgat aaagttgccg cacagatcaa
    16261 gttcggtttc tccgtcaata cctgggcggt tggcgatctg gtgcaggtgg tgaactccat
    16321 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    16381 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    16441 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    16501 tttgcacggt ctgaaacagc ttccaggtct ggccgtacag cgtctgatgc agcagggtta
    16561 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    16621 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    16681 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    16741 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    16801 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    16861 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    16921 actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    16981 gtggatcctt gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    17041 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    17101 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    17161 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    17221 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattgttttc cccctcgccc
    17281 ctttggggag agggccgggg tgaggggaaa cgacctctca ggagtcaaca tgttagaaga
    17341 tctcaaacgc caggtattag aagccaacct ggcgctgcca aaacacaacc tggtcacgct
    17401 cacatggggc aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga tcaaaccttc
    17461 cggcgtcgat tacagcgtca tgaccgctga cgatatggtc gtggttagca tcgaaaccgg
    17521 tgaagtggtt gaaggtacga aaaagccctc ctccgacacg ccaactcacc ggctgctcta
    17581 tcaggcattc ccctccattg gcggcattgt gcatacgcac tcgcgccacg ccaccatctg
    17641 ggcgcaggcg ggtcagtcga ttccagcaac cggcaccacc cacgccgact atttctacgg
    17701 caccattccc tgcacccgca aaatgaccga cgcagaaatc aacggcgaat atgagtggga
    17761 aaccggtaac gtcatcgtag aaacctttga aaaacagggt atcgatgcag cgcaaatgcc
    17821 cggcgttctg gtccattccc acggcccgtt tgcatggggc aaaaatgccg aagatgcggt
    17881 gcataacgcc atcgtgctgg aagaggtcgc ttatatgggg atattctgcc gtcagttagc
    17941 gccgcagtta ccggatatgc agcaaacgct gctggataaa cactatctgc gtaagcatgg
    18001 cgcgaaggca tattacgggc agtaatgact gtataaaacc acagccaatc aaacgaaacc
    18061 aggctatact caagcctggt tttttgatgg attttcagcg tggcgcaggc aggttttatc
    18121 ttaacccgac actggcggga caccccgcaa gggacagaag tctccttctg gctggcgacg
    18181 gacaacgggc cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt tattcccgcc
    18241 gatcaggttc cccgcgctca gcatattttg cagggtgaac aaggctttcg cctgacaccg
    18301 ctggcgttaa aggattttca ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc
    18361 caattgatga attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat
    18421 gtgcgcccgc cagaacgcta tctgatggag cggtttatca cctcaccggt gtgggtcgag
    18481 ggtgatatgc acaatggcac tatcgttaat gcccgtctga aaccgcatcc cgactatcgt
    18541 ccgccgctca agtgggtttc tatagatatt gaaaccaccc gccacggtga gctgtactgc
    18601 atcggcctgg aaggctgcgg gcagcgcatc gtttatatgc tggggccgga gaatggcgac
    18661 gcctcctcgc ttgatttcga actggaatac gtcgccagcc gcccgcagtt gctggaaaaa
    18721 ctcaacgcct ggtttgccaa ctacgatcct gatgtgatca tcggttggaa cgtggtgcag
    18781 ttcgatctgc gaatgctgca aaaacatgcc gagcgttacc gtcttccgct gcgtcttggg
    18841 cgcgataata gcgagctgga gtggcgcgag cacggcttta aaaacggcgt cttttttgcc
    18901 caggctaaag gtcggctaat tatcgacggt atcgaggcgc tgaaatccgc gttctggaat
    18961 ttctcttcat tctcgctgga aactgtcgct caggagctat taggcgaagg aaaatctatc
    19021 gataacccgt gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg
    19081 ctggcaactt ataacctgaa agattgcgag ctggtgacgc agatcttcca caaaactgaa
    19141 atcatgccat ttttactcga acgagcaacg gtgaacggcc tgccggtgga tcggcacggc
    19201 ggttcggtgg cagcgtttgg tcatctctat tttccgcgaa tgcatcgcgc tggttatgtc
    19261 gcgcctaatc taggcgaagt gccgccgcac gccagccctg gcggctacgt gatggattca
    19321 cggccagggc tttatgattc agtgctggtg ctggactata aaagcctgta cccgtcgatc
    19381 atccgcacct ttctgattga tcccgtcggg ctggttgaag gcatggcgca gcctgatcca
    19441 gagcacagta ccgaaggttt tctcgatgcc tggttctcac gggagaaaca ttgcctgccg
    19501 gagattgtga ctaacatctg gcacgggcgc gatgaagcca aacgtcaggg taataaacca
    19561 ctttcgcagg cgctgaagat cattatgaat gccttttatg gcgtgcttgg caccaccgcc
    19621 tgccgctttt tcgatccgcg cctggcatcg tcgatcacca tgcgtggtca tcagatcatg
    19681 cggcaaacca aagcattgat tgaagcgcag ggctacgacg ttatctacgg agataccgac
    19741 tcgacttttg tctggctaaa aggtgcacat tcggaagaag aggcggcgaa aatcggtcgt
    19801 gcactggtgc agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca acggctgacc
    19861 agcgcattag aactggagta tgaaactcat ttctgccgtt ttcttatgcc aaccattcgc
    19921 ggagctgata ccggcagtaa aaagcgctac gccgggctga ttcaggaggg cgacaagcag
    19981 cggatggtgt ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct ggcccagcag
    20041 tttcagcagg agctgtacct gcgtatcttc cgcaacgagc catatcagga gtacgtccgc
    20101 caaaccatcg acaaactgat ggcgggtgaa ctggatgcgc ggctggttta ccgtaaacgc
    20161 cttcgccgtc cgctgagcga atatcagcgt aatgttccgc ctcatgtacg cgccgctcgc
    20221 cttgccgatg aagaaaatca aaagcgcggt cgccccttgc aatatcagaa tcgcggcacc
    20281 attaagtacg tatggaccac caacggcccg gagccgctgg actaccaacg ttcaccactg
    20341 gattacgaac actatctgac ccgccagcta caacccgtgg cggagggaat actccctttt
    20401 attgaggata attttgctac acttatgacc gggcaacttg ggctattttg agcaaaaaaa
    20461 agagttcgcc agataccatt ttgatgcgtg acgaatgctt tgccatccag taccatagcg
    20521 ccctttccat tcctggacct gaataacgcc actacctcat aagcacggta gcgggtggtt
    20581 attgcctgca attaaagata tagagccgaa cacatatgcc ttttacactt ggtcaacgct
    20641 ggatcagcga tacagaaagc gaattgggac ttggaaccgt tgtcgcggtg gatgcgcgaa
    20701 ctgtcacttt acttttccca tctactggtg aaaaccgtct gtacgcacgc agtgattccc
    20761 ccgtgacccg cgtgatgttc aaccctggtg ataccattac cagccatgac ggctggcaga
    20821 tgcaagtcga agaagtaaaa gaagaaaatg gcttgctgac ctatatcggt actcgcctgg
    20881 atactgaaga gtccggcgta gccctgcgtg aagttttcct tgatagcaaa ctggtgttca
    20941 gcaaaccgca ggaccgtctg tttgccgggc agattgaccg tatggaccgc tttgcgctgc
    21001 gttatcgcgc gcgtaagtat tccagcgaac agttccgtat gccgtacagc ggcctgcgcg
    21061 gtcagcgtac cagcctgatc ccgcatcagc tcaacatcgc tcatgatgtt ggtcgccgcc
    21121 acgcgccgcg cgttctgctg gctgacgaag tgggtttagg gaaaaccatt gaagccggga
    21181 tgatcctgca tcagcaactg ctctctggcg ctgctgaacg tgtgctgatt atcgtcccgg
    21241 aaaccttaca gcatcagtgg ctggtagaaa tgctgcgccg tttcaacctg cgttttgcgc
    21301 tgtttgatga tgagcgttat gccgaagctc agcacgatgc ttacaacccg ttcgacaccg
    21361 aacagctggt gatttgctcg ctggattttg cccgtcgtag caaacagcgc ctggaacatc
    21421 tctgtgaagc cgaatgggac ctgctggtgg tcgatgaagc gcatcacctg gtgtggagcg
    21481 aagacgcgcc gagccgcgaa tatcaggcta ttgaacaact ggcagagcac gtgccgggcg
    21541 ttctgctgct gaccgcaacc ccggaacagt tgggaatgga aagccacttc gcccgtctgc
    21601 gtctgctgga cccgaaccgt ttccacgatt ttgcgcagtt cgttgaagag cagaaaaatt
    21661 atcgtccggt tgcggacgcc gttgccatgc tgctggcagg taacaaactg agcaatgacg
    21721 aactgaacat gcttggcgag atgatcggcg agcaggatat cgagccgctg ttgcaagcag
    21781 caaacagcga cagcgaagat gcccagagcg cccgtcagga actggtttcg atgctgatgg
    21841 atcgccacgg caccagccgc gtgctgttcc gtaacacccg taacggtgtg aaaggattcc
    21901 cgaaacgcga gctgcacacc attaagctgc cgctaccgac gcagtatcag acggctatta
    21961 aagtctccgg cattatgggc gcacgtaaaa gtgcggaaga tcgtgctcgc gatatgctct
    22021 acccggagcg tatttatcag gaatttgaag gtgataacgc cacctggtgg aacttcgatc
    22081 cgcgcgttga gtggctgatg ggctacctga ccagccatcg ctctcagaaa gtgctggtga
    22141 tctgcgccaa agctgccact gcgctgcaac tggagcaggt actgcgcgaa cgtgaaggta
    22201 ttcgcgctgc ggtgttccac gaaggtatgt cgattatcga acgtgaccgc gctgccgcct
    22261 ggtttgccga agaagacacc ggcgcacagg tactgctgtg ctcagaaatc ggttctgaag
    22321 gacgtaactt ccagttcgcc agccacatgg taatgtttga cctgccattc aacccggatc
    22381 tgctggagca gcgtattggt cgtctggatc gtatcggtca ggcgcacgat attcagatcc
    22441 atgtgcctta tctggaaaaa actgctcagt cggtgctggt gcgctggtat cacgaaggtc
    22501 tggatgcatt cgagcacacc tgcccgaccg gacgcactat ttacgatagc gtctacaacg
    22561 atctgattaa ctatctggct tcaccggatc aaaccgaagg ctttgacgat ctgatcaaaa
    22621 actgccgcga gcaacatgaa gcgctgaaag cacaactgga acaggggcgt gatcgcctgc
    22681 tggaaatcca ctccaacggt ggcgaaaaag cccaggcact ggcagaaagc attgaagagc
    22741 aggatgacga taccaacctg atcgccttcg ccatgaacct gttcgatatt atcggtatca
    22801 atcaggacga tcgcggcgac aacatgatcg tgctgacccc gtccgatcat atgctggtgc
    22861 cggacttccc tggcctgtcg gaagatggca tcaccatcac ctttgatcgt gaagtggcgc
    22921 tggcgcgtga agatgctcag tttattacct gggaacatcc gctgatccgc aacggtctgg
    22981 atctgattct ttctggcgat accggtagca gcacgatttc actgttaaaa aacaaagcgt
    23041 tgccggtagg tacgctgttg gtggaactga tttacgtggt cgaagcccag gcgccgaagc
    23101 agttgcagct caaccgcttc ctgccaccga cgccggtacg tatgctgctg gataaaaacg
    23161 gcaacaacct ggcggcccag gtggagtttg aaacctttaa ccgccagctt aacgcggtta
    23221 accgtcacac cggcagcaaa ctggttaacg ccgtgcagca ggatgttcac gctatccttc
    23281 agctgggtga agcgcagatc gagaaatctg cccgtgcatt gattgatgcc gcacgtaacg
    23341 aagccgacga aaaactgtct gccgagctgt ctcgtctgga agcgctgcgc gcagtgaacc
    23401 cgaacattcg tgacgacgaa ctgaccgcca ttgagagcaa ccgtcagcag gtaatggaaa
    23461 gcctggatca ggcaggctgg cgtctggatg ccctgcgttt gatcgttgta acgcatcagt
    23521 aacggagccg aaaatgggga tggaaaacta caatccaccg caggaaccct ggttggttat
    23581 cctgtatcag gatgaccaca ttatggtggt taacaagccg agcggtttgt tgtctgtgcc
    23641 gggtcgtctg gaagagcaca aagatagcgt gatgacgcgc attcagcgtg attatccgca
    23701 ggcagaatcg gtgcatcgtc tggatatggc taccagcggc gtgattgtag tggcgctgac
    23761 caaagccgcg gagcgggagt taaaacgcca gttccgcgag cgcgagccga aaaagcagta
    23821 tgtggcccgc gtctgggggc atccatcccc cgcagaaggt ctggtggatc tgccgctgat
    23881 ttgcgactgg ccaaaccgcc cgaaacagaa agtctgttac gaaacgggta aacctgcgca
    23941 gacggaatat gaagtggtgg agtatgtggc ggataacacg gcaagagtgg tgttaaaacc
    24001 gattaccggg cgttcgcatc aattgcgtgt gcatatgctg gcgctgggtc atccgattct
    24061 cggcgatcgt ttttatgcat caccagaagc gagagcgatg gcaccacgtt tgttgctaca
    24121 tgcagagatg ttgacgatta cccatccggc gtatggcaat agtatgacgt ttaaagcgcc
    24181 agcggatttt taatcgtcac gctggcgcag ttcggtaggc ggataaggcg ttcacgccgc
    24241 atccggcaat tgtgcgccga ttgcctgatg cgacgcgggc cgcgtcttat caggcctaca
    24301 gtttaaaact tatttaaacc ctttctgctg ctttatcagc tcatatgcct gctgaatttc
    24361 ctgcgctttc tgcttcgcca tctccatcat ctcaggcggc aaacctttcg ccaccagctt
    24421 atcgggatgg tgttcactca tcagcttacg gtaggcacgt ttgatggtgg tcgcatcatc
    24481 cgtcggcttc acgcccagca cattacaggc atcttccagc gtcgggccac gctgcgcttg
    24541 ctgccagtta ccaccgccag attgctgctg ataaccgccg ccaaactgtg caccgccctg
    24601 catcatgcgc aaaaactggt caaactgggc gcgggagatc cctaattctt cagcaatgac
    24661 atacagcacc gcccgttcat tcgggtgcag tgaaccatca gcaaacgccg cctgaatctg
    24721 gatctccaga aacatacgaa ttaagtcaaa acgaccaaag cagacactgc gaaactggcg
    24781 catcttttcg cgcagcgggt aattatctga ttttcccact cggaacgcgt tttgcgccgc
    24841 agtacgggaa gcgccatgaa ggttcattcg gtccatcaac tggctggcga tatgaatatc
    24901 agcctccgtg acgcgacctt tggatttggt taaatgcccc atcacttcaa aagtggtggc
    24961 aaaaaacagc gcctgacgct cacgctggtt ggcgaaccac gccattttac ggctacgggc
    25021 tttatcaaac atatggccaa ttaacaggcc taacactacg ccccaaaagc cgccgcccat
    25081 cagtaaggcc acggccacgc caatgatttt tccccaatac tgcatatatt ccccaaatcg
    25141 acacacggat atcagggcta tctcccacaa tataaaggtg cttttaccgt tttccggctg
    25201 cggtcaattg tgggacatcg cctataattt gcattatcat acctgtcatt caatgccgtg
    25261 cctaacacca ctgacgctat tcggacagga ttaacactag cgtagagatg acgagtacgt
    25321 tagtctctga ccgtttgtca cgcgcaacgt taccgatgat ggaacaataa aatcaacgta
    25381 tgaaaaaacg tatccccact ctcctggcca ccatgattgc caccgccctt tatagtcaac
    25441 agggactggc agccgacctc gcctcacagt gcatgttggg cgtgccaagc tatgaccgtc
    25501 ctctggtaca gggcgatacc aatgacttac ccgtgactat caatgctgac cacgcgaaag
    25561 gggactaccc ggatgacgcc gtgtttactg gcagcgtgga tatcatgcag ggtaacagcc
    25621 gtctgcaggc cgacgaagtg cagctccatc aaaaagaggc accaggacaa ccggagccgg
    25681 tacgtaccgt tgatgcgctc ggtaatgtcc attacgacga taaccaggtg atcctcaaag
    25741 ggccgaaagg ctgggcgaat ctgaacacca aagataccaa cgtctgggaa ggtgattacc
    25801 agatggtggg tcgccagggt cgcggtaaag cggacctgat gaaacaacgt ggcgaaaacc
    25861 gctataccat tctggataac ggtagcttta cctcctgtct gccgggttct gacacctgga
    25921 gcgtggtagg tagcgaaatt attcatgacc gcgaagaaca agttgcggag atctggaacg
    25981 cccgctttaa ggtgggtccg gtaccgatct tttatagccc ctatttgcag ttgccggtgg
    26041 gtgacaaacg tcgctctggt ttcttgatcc cgaacgccaa gtacaccacc accaactact
    26101 ttgagttcta cctgccatat tactggaaca tcgcgccaaa tatggatgcc accatcacgc
    26161 cgcattatat gcatcgtcgt ggcaacatca tgtgggagaa cgaattccgc tacctctccc
    26221 aggcgggcgc tggcttgatg gaactggact atctgccttc agataaagtc tatgaagatg
    26281 aacccccgaa cgatgacagt tcacgtcgtt ggttattcta ctggaaccac tccggggtca
    26341 tggatcaggt gtggcgtttc aacgtcgact acaccaaggt cagcgatcct agctacttca
    26401 atgatttcga taacaagtac ggttccagta ctgacggcta cgcaacgcaa aaattcagcg
    26461 ttggctatgc ggtgcaaaac ttcaatgcca ccgtttcaac caagcagttc caggttttca
    26521 gcgaacagaa caccagtagc tactcggcag agccgcagtt agacgttaat tactaccaga
    26581 atgatgttgg tccatttgat acgcgtattt acggccaggc agtgcacttt gttaacacca
    26641 gagacgacat gcctgaagca acccgtgttc acctggaacc gaccatcaat ttgccgctct
    26701 ctaataactg gggcagcatc aataccgaag cgaagttgct ggcaacccat tatcagcaaa
    26761 ccaatcttga ctggtataac tccagaaaca cgaccaagct ggacgaatcc gttaaccgcg
    26821 taatgccgca attcaaagtt gacggcaaaa tggtctttga acgcgatatg gaaatgctgg
    26881 ctccgggtta tacccaaacg ctggaaccgc gcgcgcagta tttgtacgtg ccgtatcgcg
    26941 atcagagcga catctataac tacgactcgt ctctgctgca atctgactac tctggcctgt
    27001 tccgggaccg gacttacggc ggtcttgacc gtattgcctc cgctaaccag gtgacgaccg
    27061 gtgtcacatc tcgcatatat gatgatgctg ccgttgaacg ttttaatatt tccgttggtc
    27121 aaatctacta tttcacggag tctcgcactg gcgatgacaa cataacatgg gagaatgacg
    27181 acaaaacggg ttcactggtg tgggcaggcg atacttactg gcgtatctcc gagcgttggg
    27241 gattgcgtgg cgggattcag tacgatacac gtctggataa cgtagcgacc agtaactcca
    27301 gcattgaata ccgtcgggat gaagaccgtc tggtacagct gaattaccgt tacgccagcc
    27361 cggaatatat tcaggctacg ctgcctaagc actattccac tgctgagcaa tataagaatg
    27421 gtatttcgca ggtaggtgct gtcgccagct ggccaattgc cgatcgttgg tccattgttg
    27481 gggcctacta ctacgacacc aatgcgaaca agcaagccga ctctatgtta ggtgtgcaat
    27541 acagctcctg ctgctatgca attcgcgtcg gttacgagcg gaagctgaac ggttgggata
    27601 acgataaaca acatgcggta tatgacaacg caatcggctt taacatcgaa cttcgcggcc
    27661 tgagctccaa ctacggtctg ggtacgcaag agatgctgcg ttcgaacatt ctgccgtatc
    27721 aaaacacttt gtgatttgtt gatttaccac gtaatccgca gtgcggttaa ttgaaatgga
    27781 aaaagtatga agaactggaa aacgctgctt ctcggtatcg ccatgatcgc gaataccagt
    27841 ttcgctgccc cccaggtagt cgataaagtc gcagccgtcg tcaataacgg cgtcgtgctg
    27901 gaaagcgacg ttgatggatt aatgcagtcg gtaaaactga acgctgctca ggcaaggcag
    27961 caacttcctg atgacgcgac gctgcgccac caaatcatgg aacgtttgat catggatcaa
    28021 atcatcctgc agatggggca gaaaatggga gtgaaaatct ccgatgagca gctggatcag
    28081 gcgattgcta acatcgcgaa acagaacaac atgacgctgg atcagatgcg cagccgtctg
    28141 gcttacgatg gactgaacta caacacctat cgtaaccaga tccgcaaaga gatgattatc
    28201 tctgaagtgc gtaacaacga ggtgcgtcgt cgcatcacca tcctgccgca ggaagtcgaa
    28261 tccctggcgc agcaggtggg taaccaaaac gacgccagca ctgagctgaa cctgagccac
    28321 atcctgatcc cgctgccgga aaacccgacc tctgatcagg tgaacgaagc ggaaagccag
    28381 gcgcgcgcca ttgtcgatca ggcgcgtaac ggcgctgatt tcggtaagct ggcgattgct
    28441 cattctgccg accagcaggc gctgaacggc ggccagatgg gctggggccg tattcaggag
    28501 ttgcccggga tcttcgccca ggcattaagc accgcgaaga aaggcgacat tgttggcccg
    28561 attcgttccg gcgttggctt ccatattctg aaagttaacg acctgcgcgg cgaaagcaaa
    28621 aatatctcgg tgaccgaagt tcatgctcgc catattctgc tgaaaccgtc gccgatcatg
    28681 actgacgaac aggcccgtgt gaaactggaa cagattgctg ctgatatcaa gagtggtaaa
    28741 acgacttttg ctgccgcagc gaaagagttc tctcaggatc caggctctgc taaccagggc
    28801 ggtgatctcg gctgggctac accagatatt ttcgatccgg ccttccgtga cgccctgact
    28861 cgcctgaaca aaggtcaaat gagtgcaccg gttcactctt cattcggctg gcatttaatc
    28921 gaactgctgg atacccgtaa tgtcgataaa accgacgctg cgcagaaaga tcgtgcatac
    28981 cgcatgctga tgaaccgtaa gttctcggaa gaagcagcaa gctggatgca ggaacaacgt
    29041 gccagcgcct acgttaaaat cctgagcaac taatggttaa aacccaacgt gttgtgatca
    29101 ctcccggcga gcccgccggg attggcccgg acttagttgt ccagcttgca cagcgtgagt
    29161 ggccggtcga actggttgtt tgtgccgatg ccactctcct taccgaccgg gcagcgatgc
    29221 tcggtttgcc gctcaccctc cgcccttatt cccccaactc ccctgcacaa ccgcaaacta
    29281 cgggcacatt aacgctactt cctgtcgcgc tacgtgaatc tgtcactgcg gggcagttag
    29341 cgattgaaaa tggacattac gtggtggaga cgctggcgcg agcgtgtgat ggctgtctga
    29401 acggtgaatt tgctgcgctg atcacaggcc ccgtgcataa aggcgtcatt aacgacgcag
    29461 gcattccgtt taccggtcat accgagtttt tcgaagagcg ttcgcaggcg aaaaaagtgg
    29521 tgatgatgct ggcgaccgaa gaacttcgcg tggcgctggc aacgacgcat ttaccgctgc
    29581 gcgatatcgc agatgctatc acccctgcac ttttgcacga agtgattgct attttgcatc
    29641 acgatttgcg tacaaaattt ggtattgccg aaccgcgcat tctggtctgc gggctgaatc
    29701 cgcacgcggg cgaaggcggt catatgggta cggaagagat agacaccatt attccggtgc
    29761 tcgacgagct gcgggcgcag gggatgaaac tcaacgggcc gctgcctgcc gataccctgt
    29821 ttcagccgaa atatctcgat aacgccgacg ccgtgctggc gatgtaccac gatcagggtc
    29881 ttcccgtgct aaaataccag ggcttcgggc gcggtgtgaa cattacgctg ggcctgccct
    29941 ttattcgcac atcagtggac cacggcaccg cgcttgaact ggcgggacgt ggcgaagccg
    30001 atgtcggcag ttttattacg gcgcttaatc tcgccatcaa aatgattgtt aacacccaat
    30061 gaataatcga gtccaccagg gccacttagc ccgtaaacgc ttcgggcaaa actttctcaa
    30121 cgatcagttc gtgatcgaca gcattgtttc tgccattaac ccgcaaaagg gccaggcaat
    30181 ggtcgaaatc ggccccggtc tggcggcatt gaccgagccg gtcggcgaac gtctggacca
    30241 gctgacggtc atcgaacttg accgcgatct ggcggcacgt ctgcaaacgc atccattctt
    30301 aggcccgaaa ctgacgattt atcagcagga tgcgatgacc tttaactttg gcgaactggc
    30361 cgagaaaatg ggtcagccgc tgcgtgtttt cggcaacctg ccttataaca tctccacgcc
    30421 gttgatgttc catctgttta gctatactga tgccattgcc gacatgcact ttatgttgca
    30481 aaaagaggtg gtgaatcgtc tggttgcagg accgaacagc aaagcgtatg gtcgattaag
    30541 cgtcatggcg caatactatt gcaacgtgat cccggtactg gaagttccgc cgtcagcctt
    30601 tacaccacca cccaaagtgg attccgccgt cgtgcgcctg gttcctcatg caacgatgcc
    30661 tcacccggtt aaagatgttc gtgtgttgag ccgcatcacc accgaagcct ttaaccagcg
    30721 tcgtaaaacc attcgtaaca gcctcggcaa cctgtttagc gtcgaggtgt taacgggaat
    30781 ggggatcgac ccggcgatgc gagcggaaaa tatctctgtc gcgcaatatt gccagatggc
    30841 gaactatctg gcggagaacg cgcctttgca ggagagttaa cgatgatcaa ttcgccccga
    30901 gtgtgtattc aggttcaaag cgtctacatt gaggcacaat cttcacctga taatgaacgt
    30961 tacgtttttg cttataccgt aaccatacgc aatctggggc gagcgccagt gcagttgttg
    31021 gggcgttact ggctgatcac caatggcaat ggccgtgaaa ccgaagtcca gggcgaagga
    31081 gtggttggcg tccagccact tatcgcgcct ggcgaagagt accagtacac cagcggtgca
    31141 atcattgaaa ccccgctggg caccatgcag ggtcactacg aaatgatcga tgaaaatggc
    31201 gtccctttca gcatcgacat tcccgtattc cgactcgccg ttcccacact cattcattaa
    31261 aagaatatgg cgacatacct tattggcgac gttcatggtt gttacgatga actgttcgca
    31321 ttgctgcata aagtagaatt tacccctggg aaagataccc tctggctgac gggcgatctg
    31381 gtcgcgcgcg ggccgggttc gctggatgtt ctgcgctatg tgaaatcctt aggcgacagc
    31441 gtacgtctgg tgctgggcaa tcacgatctg catctgctgg cggtatttgc cgggatcagc
    31501 cgcaataaac cgaaagatcg cctgacaccg ctgctggaag cgccggatgc cgacgagctg
    31561 cttaactggc tgcgtcgcca gcctttgctg caaatcgacg aagagaaaaa gttggtgatg
    31621 gcccacgcag ggatcacgcc gcagtgggat ctacagaccg ccaaagagtg cgcgcgcgat
    31681 gtagaagcgg tgctatcgag tgactcctat cccttctttc tcgacgctat gtacggcgat
    31741 atgccaaata actggtcacc ggaattgcgg gggctgggaa gactgcgttt tatcaccaac
    31801 gcctttaccc gtatgcgttt ttgctttcca aacggtcaac tggatatgta cagcaaagaa
    31861 tcgccggaag aagcccctgc cccactgaaa ccgtggtttg cgattcctgg ccctgtcgct
    31921 gaagaataca gcatcgcctt tggtcactgg gcatcgctgg aaggcaaagg tacgccggaa
    31981 ggtatttacg cgctggatac cggctgctgc tggggtgggt cattaacctg cctgcgctgg
    32041 gaagataagc agtattttgt ccagccgtcg aaccggcata aggatttggg tgagggagag
    32101 gcggtcgcgt cttaacgaat taccattcag ggaggaataa tggctgacta ttttgaaata
    32161 cgtgctgatt acaatcagca caccatcacg atttatcagg cttacaatga tgcaatcgct
    32221 gacgtcgccg tacgtgacgg gcgatttggc gcaccttttt cattcaaccg catgacctgg
    32281 attaaaccct cttttatgtg gatgatggag cgtagcaact ggggactgaa aaaagatcaa
    32341 caacacattc tcgcaatccg aatcaaacgc accttctttg atacattgct ggaacaagcg
    32401 gtgctaacca cgccggaagc ccatgtgtat ccacatgcag gaatttggga aacgttattt
    32461 gcacaagcaa atgtgtatgt tcagtgggac ccagaacgca gcataaatgg taaaaaactg
    32521 gagcaccgat cattacagct tggtatttct cgtaacttga tcagtcaatt taacgaagac
    32581 gcgattgttg ctattgatga tctgacgccg ctggtgcgaa agtgtcataa ccttttgata
    32641 aacggcaaaa caacacaggc gaaatccttc ctgccgccag aaaaaattta cccagttagt
    32701 gcagcagcga gaaaggcttt gggaatgaaa aattcctgag acaaatacgc acctattaag
    32761 cggaaaatat ctaagtgcga caaatcaaca ccgggcttaa taccagagaa attattctgg
    32821 aaatgattga cgaagcagat gcccccccgt ttgctgagca gcggacctgg catcgcaggc
    32881 gaatcgcttt tctatgtgat tgcatcagcg ttgctgtcga ctacggcttc ttctgttctg
    32941 tctctcagca gtcaataatg aacataacca agatggctga agatgtatgg ataacagaaa
    33001 gtaaaacgac ccaactcatt gatatttggc aacaacttaa acagctttat ccgccagaaa
    33061 ctattcccta cgcccagaag ttacccacta aggaattgct tgaactgcta ctttctagtc
    33121 ctgacgatct tcgaataatt gaaattcctg acatcatcgg tgatgttttt gatgaccaaa
    33181 cctggattcg attattcaaa aaacactttg ctgatgttat tcagctacga tgtgatgtgt
    33241 aacaatccaa aatatatcgc cagacgatag accaaaaaga aagtatttag cagatactca
    33301 acattctgac ttctgtacag aaatagcaga gtttaaaaac ttaaaacttc cggtactcat
    33361 cagagaaaca tccagtttct tctgcaaatt catttaatgc cgagatggca tcctcgttta
    33421 cttcattcca ctgtagagtg gcctgaaggt ttagacctac gcaatgagcg cgactcacaa
    33481 aatcgcgctc actggtaaaa tcaaacgtct tatcgttcaa tttgtagtcc tggcaaaaaa
    33541 ttaccgccgc tccagaatct caaagcaata gctgtgagag ttctgcgcat cagcatcgtg
    33601 gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc
    33661 ttccacttct gcgtcgatat gcgtcagata cagtttctgc gcttttggca ggaactgctc
    33721 ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc
    33781 ttcatccacc gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat
    33841 aatatttttg cgtcctggca acggacgacc gattgattcc caggtatggc ggcccataat
    33901 cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg
    33961 catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat
    34021 tgagatttcc cgataaaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac
    34081 tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctctcc
    34141 ctggactcgc cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag tgtgatttac
    34201 gtcactcttt attaggatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt
    34261 ttaccttctt cggttccctg ccagccatgt cgttgaatta atgacagatg ttcgcggtcc
    34321 tcggtaatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg
    34381 gtgtcgttct caaccattgc catctcttcc accatctgaa tattaaagcg gcggaacaca
    34441 tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagtgc cagacgcccg
    34501 gttttcagcg caccttcgaa ggtttcacgc tccggctttt caacgcctgc ctgacgcaaa
    34561 cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaaatgttct
    34621 ttcaccatct ccgtcagttg caggttggtt tgcggatcat caatagcatt aatcagcact
    34681 tccgctttcg ccgctccggc agattccagt aaatccatcc gcgttgcatc gccataaaac
    34741 actttcatac caaatttacg cagcgtttcg atatggtccg gatcgtgatc gagcaccacc
    34801 attttcaccc cgctggagag cagtaaacgt ccggtaatct gcccaaaacg accgaatccg
    34861 gcgataatca cgcgcggctg ttcttcgtcg atctcatcgg cttcacgcgc ttcctcagta
    34921 gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc catcgacagc
    34981 gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc
    35041 gcgccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc aaaccaacga
    35101 cgctgtttat ttggcacttg caacggtcgg gcaatcagcc acagcatggc gattttgatg
    35161 atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag cgtgccaaag
    35221 tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt aaatggttcg
    35281 atatcgctct ccagcgcatg acggtattcc gagcttgcca gcagtacgcc cgccagaaac
    35341 gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc aaacacgagg
    35401 aataacgcca cggcactaaa cacttcccgc aagccagagc gggctacaaa acgcagcgcc
    35461 ggacgcgtga catagcgccc cagcaatacc accagcacca gcgcacccgc cacttttaac
    35521 gccgagagag caaatgcgcc catcgtcgtc gaggcactgc tcgttgccag tagcggaatc
    35581 atcgccacca gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcga
    35641 cccatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat cgccgtagag
    35701 gagagcgcta gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc aagtaacatg
    35761 cagaacagcc ccagcaggcc gccgcaaatc accatctgca atgcgccacc gccgaacact
    35821 gccgcacgca gcttccacag cctttgtgga tcgagttcga ggccgataat aaacagcatc
    35881 agcaccaccc caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc
    35941 ccccacgggc caataatgca gccggcgatc aggtagccaa gtaccgatcc cagaccaaga
    36001 cgtaccgcaa tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc ctgaatcagc
    36061 gtatggctat ccatgatggg cctcctgcca ttccagcaga cgttgcttat agtgacgcgc
    36121 ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag
    36181 ccagttcagt ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc
    36241 cggatgcgca ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg cccacagcaa
    36301 atgtttgcca tgcagcgccg tgccgccatg accgtaagcc cagccgtggg agaaaacttt
    36361 atcgatccaa agtttgagga gcggaggaat gctgtaccac tgcatcgggt gctgccagac
    36421 gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca atatcgatat tgaagtcagg
    36481 atagagttga taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat
    36541 ccgtttattc gcatgggaat gatgcggata cggatgcgca taaattataa gaatcatagg
    36601 tagcccgcgt gattacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    36661 atagtcaata atattgattc agaaaagaga gaaatctgaa caatttaccc cattctctgc
    36721 ccgcggatcg tcatattgct ggtttgcgcc agtgacatcc ctcgagtctc cggggcaaac
    36781 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    36841 ataccgtaat tattgataaa gatcggcagt gcccaggtcg aaacaatggt gccaatacgg
    36901 cttaaggaca taatcacgcc cacggcagag gcgcggatat ctgtcgggaa gagttcatta
    36961 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    37021 atcactacca gccagatccc catatccggg atcagcccca aaaccgccag cgccagcgtc
    37081 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttaa ccacagcatc
    37141 ggcggaatac agccgagcat aaagaacagg ctaatcacca cgttccccag cgccgcgttt
    37201 ttgccaacac ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac
    37261 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    37321 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    37381 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    37441 cagcggggag attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgcc
    37501 gcgctaccca gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccaga
    37561 tcggcgcagg tcgcgccgac ataccacatg gcggcgataa agctgatgga aaacgcccgc
    37621 tgacgggtac tggagaactc ggtgatcatt gaggtggcga tgggataatc tgcaccgatg
    37681 acaatgccga taagtacccg catcaccaac agttcgacgg gggatgaaac aaacatcgtc
    37741 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    37801 ttatcggaaa tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    37861 cccaacaagc caatccagtc agcgtccagt tttagcgccg gagtcagttg ctccagcgcc
    37921 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    37981 atgcggcggt gaattgagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    38041 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    38101 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    38161 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    38221 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagggc
    38281 ctgtttatcg gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    38341 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    38401 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    38461 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    38521 agttccgggt agccgctaaa catgcgtggg ttatcaagga aggccggtag tttctggtac
    38581 atacgcatat cgcgcagcgg gccactctca agatgctgac gatattccgc gagtttttgc
    38641 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    38701 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    38761 tcaccggcaa tcaatacgcc gtcaccaacc aactccggca gcatgttgat gcctgcttcc
    38821 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgccactgcc
    38881 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt tttttgcgtc atgcagatga
    38941 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    39001 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    39061 aactgaaaac ggtcttcgat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    39121 gccacatccg tcggtttaac gcgttttgcc atccccagct tttcggcaag gatggagttc
    39181 actccatcag caaggatcac cgttttcgct tcaatcacat cgccatcggc ttctacaccg
    39241 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    39301 cccgcttctt cggcctgctc catcagccag gcatcaaatt tactgcgcaa aacggagtaa
    39361 gaacgctggg atggcgaggt ttcgtcacca ttgcagtagt ccatagtcat cgctgacttt
    39421 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggagtcggcg
    39481 aaaccaggaa taatgtgttc caggctgtgg gcatagagac gcccgccggt gacgttcttg
    39541 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    39601 agtgcggcaa ccgaaccggc aagcccagca ccgacgatga tggcgtcaaa gatatcttcg
    39661 gacataagag ctccctgtca gcggcagggc caaaatgcag ccctgccaga gtggatcaac
    39721 gcgctaaagc tgcggtcagc gccggaagga tcttcacggc gtcaccaaca atgccgtaat
    39781 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgatg
    39841 cattagcacc aaccatgtgc tggatctgcc cggagatccc caccgccagg tacagttcag
    39901 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    39961 ccgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    40021 gcgcaatgtt ctctttgctg ccaataccgc gaccgacgct gaccaccaga cgggctttgt
    40081 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    40141 gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    40201 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    40261 tcgccagacc accgtaaacc atgtgtttca ctgtcgcttt accgtcctgt acgctgacgg
    40321 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    40381 tgccgcgacg ggtgtttggc agcagcacca ggccgtctgc gccgtgctgg cgaatagtgt
    40441 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    40501 agacatgatt agcgccgagc tggattgcct gtacgccgtc ggcatcattg aggacaaagg
    40561 tgttgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaagggg
    40621 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    40681 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    40741 gcgctgacgt tcgcgctgtt tcggcgcagc aacctgttgt tcagaccagg cgtccacggc
    40801 gttaaaaccg atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    40861 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    40921 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    40981 tgcagtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    41041 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcaa
    41101 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    41161 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    41221 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggccg tcacctgcgc
    41281 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttcagat cgtattggct
    41341 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    41401 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    41461 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    41521 aataaaactt taatcaccaa tattgaaaat gtcacacgca gataaatcca actttcaata
    41581 ttgttaagtt cctcaccaat attgaaaaca tcgcgtagca aaaagaaatt ttcaatattg
    41641 ctttatggat caccaatatt gaaagtcaga tgctattttt ttactcacaa cacaacataa
    41701 caaactgatt aataatcaaa attaacgaga aaagcgccct ctattcaccc gaaacaaaaa
    41761 tgtgataaca atcacagaat gcagcttatt gaatacccat tatgagttag ccattaacgc
    41821 gtccacgagg ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc
    41881 aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg
    41941 gaatagaacc gaaggttttc tttccgccgt taataatcgt cggcatactt tgttggctta
    42001 cagtcagaga tctggatgca gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca
    42061 atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc
    42121 tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accacctgaa tttagtaccg
    42181 ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat
    42241 cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag
    42301 gggcgaaaga gttggggctg gcttacagct tgttccactg gggacctctg ccgtgggcca
    42361 cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga
    42421 ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa acacgccaaa gggttgttcg
    42481 gcactatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg
    42541 gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc
    42601 tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgctt
    42661 gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga
    42721 tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat catgaactac ttcaccgatt
    42781 cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta
    42841 aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttatgcta
    42901 tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct
    42961 tcggcatggt gctggggctg acagcgtcaa cctggatcct gtggactgta ctcggtagta
    43021 acactctgct gttgatagat aaaaacatca tcaacattcc aaatctgatc gaacagtacg
    43081 gtgtggcgcg cgccatcatt gaaacctggg ccgctctgcc actcagcacc gccaccatgt
    43141 ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata
    43201 ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaaccacct ctgctggtgc
    43261 gtatcggttg gtcaattctg gttggcatta tcggtattgt tctgctggcg ctcggcggcc
    43321 tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta
    43381 tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca
    43441 aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg
    43501 ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc
    43561 gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc
    43621 tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt
    43681 ggatggagct gggacgtctg ggggcaccaa cctatgtgct gtaccagttg ccgggcgggt
    43741 tcaacacctt cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg
    43801 gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg
    43861 gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt
    43921 gttttattac cagcagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt
    43981 ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag
    44041 tgaccaaact tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg
    44101 acgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca
    44161 aagaagagtt cgaccatgaa cgtttcctgg tagccctcac caactacggt acggcgatgt
    44221 gcgcctttga agatgcggcg cgctacgcca atcagcgcgt gcagtttggc gaggctattg
    44281 gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga
    44341 aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg
    44401 cagcgatgtg caaatacttc tgcgccaatg cggcatttga agttgtggat agcgcaatgc
    44461 aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgatc
    44521 tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca gatcctgacg ctgggtcgcg
    44581 cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgcta atgcgtctta
    44641 tcaggcctac gggagcacga atgtaggccg gataaagcgt ttacgccgca tccggcagtc
    44701 atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg
    44761 gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccgggc cgttcgccgg
    44821 gcaaatgttc gcagaatggg gtgcggaagt tatctggatc gagaacgtcg cctgggccga
    44881 caccattcgc gttcaaccga actacccgca gctctcccgc cgcaatttgc acgcgctgtc
    44941 gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga
    45001 tatcttcatc gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt
    45061 actgtggcag cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg
    45121 caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct tcagtggtta
    45181 cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta
    45241 cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag tgcgtgaaac
    45301 cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta
    45361 cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga
    45421 tccctactac gccggttgcg gtctgtataa atgcgccgac ggctacatcg tgatggaact
    45481 ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac atctgctcgg
    45541 tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc
    45601 actggtagaa gagaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga
    45661 acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag
    45721 caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac
    45781 ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg
    45841 aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa
    45901 cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt
    45961 cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg
    46021 caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg gcggacgttt
    46081 acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt
    46141 atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc
    46201 gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt
    46261 tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg
    46321 aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaattct
    46381 atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga
    46441 cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg aaaaatcaac
    46501 aacctgccac cttgtgctat gcaccgccgc tattgactga cgatacggcg gaaattctct
    46561 tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct
    46621 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg
    46681 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
    46741 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtgcaga
    46801 agtaccgcgc caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc
    46861 cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt
    46921 cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg
    46981 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cgacgctggc
    47041 cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc
    47101 cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
    47161 tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc
    47221 tacataccgg cgataccgga tactgcgacg aagagggctt tttttatttc gtcgatcgcc
    47281 gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca
    47341 tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg
    47401 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
    47461 ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca
    47521 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
    47581 aacagcaggg aactaccctg ccagctaata atcacgctat aaaacaatat gcattttttg
    47641 cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
    47701 atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa gcgaatgcta
    47761 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
    47821 aattacgtgt cgccattatt accggtgccg gagagaagtt cttttccgcg ggctgggatt
    47881 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggatttgcgg
    47941 gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac ggctatgcct
    48001 ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca
    48061 gcttcgccct gccggaagcc aagctgggca tcgttcctga cagcggcggc gtgctgcgtc
    48121 taccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg
    48181 gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga
    48241 tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgccccgctg gcgattgcgg
    48301 cgctgaaaga gatcttccgc accaccagcg aaatgccggt agaagaagcg tatcgctata
    48361 ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca ttcggaggat gccattgaag
    48421 ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag
    48481 ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag
    48541 tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
    48601 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
    48661 cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca
    48721 cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt
    48781 gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa
    48841 agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg
    48901 cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
    48961 tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa
    49021 tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg
    49081 acctggttgc tggataagat gctacaacat cgcatctggc aagcaggacg cccggtatca
    49141 acgagataaa attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
    49201 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
    49261 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
    49321 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aacttcgcag
    49381 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
    49441 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
    49501 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
    49561 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
    49621 attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct
    49681 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc
    49741 tcatcagaga accgtattac ttattcgatc aaattagcgg atgaaaaata tccgccatga
    49801 cacgctatta tttgatctgc gcgtgcattt cctgcaccga aattactttt tcggtcgcgt
    49861 cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt
    49921 tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcgcga cggcctgaag
    49981 tggtgttgat gatgtaggta tattcgccat tcttgatgcg gtcctgaatg tgcggacggc
    50041 cttcatgcac cttgttcacc agacgcggat taatgcccgc ttcgcccagc acaatcgccg
    50101 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
    50161 cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg accgtgtttc ttcatggtgg
    50221 agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatca
    50281 cttccccggt ggagcgcatt tctggcccta acagcgggtc aacacccggg aatttattga
    50341 acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct
    50401 gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg
    50461 tggctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga
    50521 cttcgttgtt tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg
    50581 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg
    50641 gcagagaaca tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccatgatgc
    50701 cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct accgcgtcat
    50761 caaggaagtg atccagcaac actggcgcat cgttagacac gctgaccgcc gtctggaagt
    50821 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
    50881 acggacgtac caccagcggg tagccaatct ctttcgcctt ctctaccgcc atttcaatag
    50941 cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc
    51001 gttcacggtc ttctgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag
    51061 cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc
    51121 ctttcggctt ctcaatacgc acgatttcta gcacatcttc cagtgttacc ggctcgaagt
    51181 agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc cgggttacag ttaaccataa
    51241 tggtttcgta accgtcttcg cgcagtgcca gcgaggcgtg tacacagcag tagtcgaatt
    51301 cgataccctg accgatacgg ttcgggccgc cgccgagaac catgattttt tcacggtcgg
    51361 tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg
    51421 cgaactctgc cgcacaggta tccacgcgct tataaaccgg gtgcaggtca tactggtcgc
    51481 gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt tgccagacgc gcatcggcaa
    51541 agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg
    51601 ctactttctc ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatat
    51661 tggtcaggtt gaacacgccg tccacagaca ggcccgcacg gaaggcatcg gcgatgtacc
    51721 agatacgctc tgcgccagca tctttcagtt cgcgacggat tttggttaac gcttccgggt
    51781 catcgaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt
    51841 tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct
    51901 gagtggtcag acggtcgtta gcaccagcga atttttcgaa gttgaagcga ggaattttag
    51961 taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt
    52021 tcatcagttc gtcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga
    52081 aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgcgggttc atttcgataa
    52141 caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa
    52201 cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg
    52261 tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt atggatgccc atcgcatcaa
    52321 agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct
    52381 cgtactcttt ccagccaatc agcgactcat caatcagcaa ctctttggtc ggagagagat
    52441 ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata ccgccgccgc
    52501 taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg
    52561 ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct
    52621 tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcggtcgcac
    52681 caatcatagt cacgccgaac tcttccaaca cgccctgacg ctccagctcc agcgcgcagt
    52741 tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct ttttcaataa
    52801 tcttgcgtac cacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt
    52861 cggtcatgat ggtcgccggg ttggagttca ccagaatgac ccggtaaccc tcttcgcgca
    52921 gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg
    52981 ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac
    53041 tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa
    53101 cggcgcggcg tcgtgtggac cagggctggc ttcagggtgc ccctggaagc tgaacgccgg
    53161 tttatcggtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg
    53221 caggtttgct ggtaatgttg cttcgtccac cgcaaaaccg tggttctggg cggtgatcat
    53281 caccacgttt ttctcaacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat
    53341 tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc agatgccgaa
    53401 gaccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc
    53461 cgggtcgcca ggaccgttgg agaggaagat accgtctgga ttcattttca gcacatcttc
    53521 cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctgtccacca acatgcgcag
    53581 gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt
    53641 tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc
    53701 tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt
    53761 ttctaacgcc aacgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc
    53821 tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt
    53881 atggcgcttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg
    53941 caggtcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt
    54001 agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg
    54061 atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc
    54121 cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag
    54181 cgctgactta atcaaaacac cctccagaga atattcactc actttatttg catattaatt
    54241 cacaatgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg
    54301 cattctggag atatgggcgc taaaagtcaa cttaatggcg ggatttttta tattattttg
    54361 tggctcatgc atgttttaca ggcattaagc ggcaaaaaga tcatataacc tgttctggaa
    54421 aacgcttgcg cagcaagaag attgcatgaa ataaaagcga gtagggaaaa gtggaccaaa
    54481 tggtcaaaat ttacattaaa acaacaaaaa ccgaagacaa aacatgtaat ttttggcact
    54541 tata
//
LOCUS       DAZFKM010000018        48741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFKM010000018 DAZFKM010000000
VERSION     DAZFKM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            398..1615
                     /gene="alaA"
                     /locus_tag="ACHKAY_001168"
     CDS             398..1615
                     /gene="alaA"
                     /locus_tag="ACHKAY_001168"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO5699877.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1699..2298
                     /gene="yfbR"
                     /locus_tag="ACHKAY_001169"
     CDS             1699..2298
                     /gene="yfbR"
                     /locus_tag="ACHKAY_001169"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5699878.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2357..4189)
                     /locus_tag="ACHKAY_001170"
     CDS             complement(2357..4189)
                     /locus_tag="ACHKAY_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO5699879.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4276..4926)
                     /gene="hxpA"
                     /locus_tag="ACHKAY_001171"
     CDS             complement(4276..4926)
                     /gene="hxpA"
                     /locus_tag="ACHKAY_001171"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO5699880.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4937..5431)
                     /locus_tag="ACHKAY_001172"
     CDS             complement(4937..5431)
                     /locus_tag="ACHKAY_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO5699881.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5514..5969)
                     /gene="yfbV"
                     /locus_tag="ACHKAY_001173"
     CDS             complement(5514..5969)
                     /gene="yfbV"
                     /locus_tag="ACHKAY_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO5699882.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6307..7509
                     /gene="ackA"
                     /locus_tag="ACHKAY_001174"
     CDS             6307..7509
                     /gene="ackA"
                     /locus_tag="ACHKAY_001174"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO5699883.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7584..9728
                     /gene="pta"
                     /locus_tag="ACHKAY_001175"
     CDS             7584..9728
                     /gene="pta"
                     /locus_tag="ACHKAY_001175"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5699884.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9918..11438
                     /gene="yfcC"
                     /locus_tag="ACHKAY_001176"
     CDS             9918..11438
                     /gene="yfcC"
                     /locus_tag="ACHKAY_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO5699885.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11471..12013)
                     /gene="yfcD"
                     /locus_tag="ACHKAY_001177"
     CDS             complement(11471..12013)
                     /gene="yfcD"
                     /locus_tag="ACHKAY_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO5699886.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12071..12622)
                     /gene="yfcE"
                     /locus_tag="ACHKAY_001178"
     CDS             complement(12071..12622)
                     /gene="yfcE"
                     /locus_tag="ACHKAY_001178"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO5699887.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSLPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12678..13322)
                     /gene="yfcF"
                     /locus_tag="ACHKAY_001179"
     CDS             complement(12678..13322)
                     /gene="yfcF"
                     /locus_tag="ACHKAY_001179"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO5699888.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13458..14105
                     /gene="yfcG"
                     /locus_tag="ACHKAY_001180"
     CDS             13458..14105
                     /gene="yfcG"
                     /locus_tag="ACHKAY_001180"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO5699889.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14162..14524
                     /gene="folX"
                     /locus_tag="ACHKAY_001181"
     CDS             14162..14524
                     /gene="folX"
                     /locus_tag="ACHKAY_001181"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO5699890.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14545..15438
                     /locus_tag="ACHKAY_001182"
     CDS             14545..15438
                     /locus_tag="ACHKAY_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO5699891.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15486..16376)
                     /gene="rpnB"
                     /locus_tag="ACHKAY_001183"
     CDS             complement(15486..16376)
                     /gene="rpnB"
                     /locus_tag="ACHKAY_001183"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO5699892.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16573..17346)
                     /gene="hisP"
                     /locus_tag="ACHKAY_001184"
     CDS             complement(16573..17346)
                     /gene="hisP"
                     /locus_tag="ACHKAY_001184"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO5699893.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17354..18070)
                     /locus_tag="ACHKAY_001185"
     CDS             complement(17354..18070)
                     /locus_tag="ACHKAY_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5699894.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18067..18753)
                     /gene="hisQ"
                     /locus_tag="ACHKAY_001186"
     CDS             complement(18067..18753)
                     /gene="hisQ"
                     /locus_tag="ACHKAY_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO5699895.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18843..19625)
                     /gene="hisJ"
                     /locus_tag="ACHKAY_001187"
     CDS             complement(18843..19625)
                     /gene="hisJ"
                     /locus_tag="ACHKAY_001187"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO5699896.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19846..20628)
                     /gene="argT"
                     /locus_tag="ACHKAY_001188"
     CDS             complement(19846..20628)
                     /gene="argT"
                     /locus_tag="ACHKAY_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO5699897.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20894..21463)
                     /gene="ubiX"
                     /locus_tag="ACHKAY_001189"
     CDS             complement(20894..21463)
                     /gene="ubiX"
                     /locus_tag="ACHKAY_001189"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO5699898.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21558..23075)
                     /gene="purF"
                     /locus_tag="ACHKAY_001190"
     CDS             complement(21558..23075)
                     /gene="purF"
                     /locus_tag="ACHKAY_001190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO5699899.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23112..23600)
                     /gene="cvpA"
                     /locus_tag="ACHKAY_001191"
     CDS             complement(23112..23600)
                     /gene="cvpA"
                     /locus_tag="ACHKAY_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO5699900.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23859..24521)
                     /gene="dedD"
                     /locus_tag="ACHKAY_001192"
     CDS             complement(23859..24521)
                     /gene="dedD"
                     /locus_tag="ACHKAY_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO5699901.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24511..25779)
                     /gene="folC"
                     /locus_tag="ACHKAY_001193"
     CDS             complement(24511..25779)
                     /gene="folC"
                     /locus_tag="ACHKAY_001193"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO5699902.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25849..26763)
                     /gene="accD"
                     /locus_tag="ACHKAY_001194"
     CDS             complement(25849..26763)
                     /gene="accD"
                     /locus_tag="ACHKAY_001194"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO5699903.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26919..27578)
                     /locus_tag="ACHKAY_001195"
     CDS             complement(26919..27578)
                     /locus_tag="ACHKAY_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5699904.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27661..28473)
                     /gene="truA"
                     /locus_tag="ACHKAY_001196"
     CDS             complement(27661..28473)
                     /gene="truA"
                     /locus_tag="ACHKAY_001196"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO5699905.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28473..29486)
                     /locus_tag="ACHKAY_001197"
     CDS             complement(28473..29486)
                     /locus_tag="ACHKAY_001197"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5699906.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29552..30688)
                     /gene="pdxB"
                     /locus_tag="ACHKAY_001198"
     CDS             complement(29552..30688)
                     /gene="pdxB"
                     /locus_tag="ACHKAY_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO5699907.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30787..31782
                     /gene="flk"
                     /locus_tag="ACHKAY_001199"
     CDS             30787..31782
                     /gene="flk"
                     /locus_tag="ACHKAY_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO5699908.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31779..32957)
                     /locus_tag="ACHKAY_001200"
     CDS             complement(31779..32957)
                     /locus_tag="ACHKAY_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5699909.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33241..34461)
                     /gene="fabB"
                     /locus_tag="ACHKAY_001201"
     CDS             complement(33241..34461)
                     /gene="fabB"
                     /locus_tag="ACHKAY_001201"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO5699910.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34620..36626
                     /gene="mnmC"
                     /locus_tag="ACHKAY_001202"
     CDS             34620..36626
                     /gene="mnmC"
                     /locus_tag="ACHKAY_001202"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO5699911.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36747..37025)
                     /locus_tag="ACHKAY_001203"
     CDS             complement(36747..37025)
                     /locus_tag="ACHKAY_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO5699912.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37059..37607)
                     /gene="epmC"
                     /locus_tag="ACHKAY_001204"
     CDS             complement(37059..37607)
                     /gene="epmC"
                     /locus_tag="ACHKAY_001204"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO5699913.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37607..38416)
                     /locus_tag="ACHKAY_001205"
     CDS             complement(37607..38416)
                     /locus_tag="ACHKAY_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO5699914.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38416..39240)
                     /gene="mepA"
                     /locus_tag="ACHKAY_001206"
     CDS             complement(38416..39240)
                     /gene="mepA"
                     /locus_tag="ACHKAY_001206"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO5699915.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39244..40329)
                     /gene="aroC"
                     /locus_tag="ACHKAY_001207"
     CDS             complement(39244..40329)
                     /gene="aroC"
                     /locus_tag="ACHKAY_001207"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO5699916.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40364..41296)
                     /gene="prmB"
                     /locus_tag="ACHKAY_001208"
     CDS             complement(40364..41296)
                     /gene="prmB"
                     /locus_tag="ACHKAY_001208"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5699917.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41462..42013
                     /gene="smrB"
                     /locus_tag="ACHKAY_001209"
     CDS             41462..42013
                     /gene="smrB"
                     /locus_tag="ACHKAY_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO5699918.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42086..42937)
                     /locus_tag="ACHKAY_001210"
     CDS             complement(42086..42937)
                     /locus_tag="ACHKAY_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO5699919.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(42939..43478)
                     /locus_tag="ACHKAY_001211"
     CDS             complement(42939..43478)
                     /locus_tag="ACHKAY_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5699920.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43475..43963)
                     /locus_tag="ACHKAY_001212"
     CDS             complement(43475..43963)
                     /locus_tag="ACHKAY_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5699921.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(43960..44469)
                     /locus_tag="ACHKAY_001213"
     CDS             complement(43960..44469)
                     /locus_tag="ACHKAY_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5699922.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44485..45237)
                     /locus_tag="ACHKAY_001214"
     CDS             complement(44485..45237)
                     /locus_tag="ACHKAY_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5699923.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45257..47902)
                     /gene="yfcU"
                     /locus_tag="ACHKAY_001215"
     CDS             complement(45257..47902)
                     /gene="yfcU"
                     /locus_tag="ACHKAY_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO5699924.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47984..48547)
                     /locus_tag="ACHKAY_001216"
     CDS             complement(47984..48547)
                     /locus_tag="ACHKAY_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5699925.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11918 a        13204 c        12163 g        11456 t
ORIGIN      
        1 cctcattcat taattccgct taacaatagt ttcacacctt gcccccatta cgacacctcg
       61 gaaatcatca cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag
      121 tgaattctta aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttac
      181 aattaagaat cagaacaatt cactatataa cattgcatgt aaagcatata cacctcatta
      241 ttttgtcatt attaagttat taacagcaca atcaagcctt cccctctggc aaaatcttat
      301 tctgcagacc ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg
      361 cagggagtgg cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag
      421 caaattagag aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct
      481 ggaagaagaa ggtaacaagg tactgaaact gaacatcggc aacccagccc cgttcggttt
      541 tgacgcgcca gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta
      601 ttgcgattcc aaaggtcttt actccgcgcg taaagccatc atgcagcact accaggctcg
      661 tggcatgcgt gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat
      721 cgttcaggca atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga
      781 ttacccacta tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg
      841 cgatgaatcc tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg
      901 tacgcgtggg atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga
      961 gcttttaatg gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga
     1021 aatttatgac aaaattcttt acgacgacgc tgagcatcac tcaattgcgc cgctggcacc
     1081 tgacctgctg accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg
     1141 tcaggggtgg atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct
     1201 ggaaatgctg gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac
     1261 cgcgctgggt ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga
     1321 gcagcgtaac cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc
     1381 tcgtggtgcg ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga
     1441 tcagaaaatg gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc
     1501 attcaactgg ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat
     1561 cgagctgtct ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta
     1621 atttcactgc cggagattgc atccggcagc gttatcccgc cacaatgacc tgatgatgtc
     1681 atcatacgta aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa
     1741 ctcattaacc gctggccgct catgcgcaac gtgcggacgg aaaatgtgtc cgaacacagt
     1801 ttgcaggtag cgatggtcgc ccatgcgctg gcagctatca aaaatcggaa atttggcggt
     1861 aatgtcaacg ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc
     1921 accggcgatc tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag
     1981 gctattgaaa aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat
     2041 atctttgcgc cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa
     2101 caggcagatg cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat
     2161 gaattcttgc tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag
     2221 atggactact tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc
     2281 caggattcac cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg
     2341 cgacatcagg ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca
     2401 tcacgataat ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg
     2461 gacctaaaac cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg gcggatgctg
     2521 ccatcgccac gaccatcgcg aatggataag gcgacacccc catcgttttg gcagcagcca
     2581 gcgcaatcgg agccatcaac accgccgtcg cggtattaga gataaatagc ccaataaccg
     2641 ccgacaagac aaacaaacac cccagcatca tatgtggccc gtaaccaccg ccaatgtcca
     2701 tcagcccttt caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca
     2761 tcatcccaac gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat
     2821 ctatacagcg gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa
     2881 tttcatctgt cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct
     2941 ggctgtgcgc gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc
     3001 ggccctgttt ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc
     3061 ccagcagcag aggctcatcc gccagcgaac cttccagcgc cacgccattg cgctttagcc
     3121 ccaccacatt cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca
     3181 gttctgactc aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa
     3241 aatactcgcc gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat
     3301 cgacatcagc cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc
     3361 cattaacgtt cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag
     3421 cgccataacg ctcacgtaat ttgagatcat ccagccgttg acctatcatt ggcgatccgg
     3481 ggcgaatagc cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa
     3541 aggttcgacg cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac
     3601 gcatcactaa catatacaaa atacccagca ccagcacgac caggccaata ggtgttacgc
     3661 taaagaaact gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg
     3721 gcggcgtcgc caccagcgtc atcatgccgc taatcagccc ggcaaaactt aacggcatca
     3781 tcagacgtga cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga
     3841 tagcgacaac gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca
     3901 aaaccaacat tttgatttca ctgttgcccg ccactttgac cagccatgtt cccattacgg
     3961 tggcaacacc ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa
     4021 cgttaggatc agaaaagccg gaaaatactt cggggaccgt cagcgttccg cttaatgcaa
     4081 acgcgacaat aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga
     4141 cgatggcaac ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt
     4201 gttaattgag ggggatgact tgattctgcc ataaaaaagc cccgacgaga cggggctaaa
     4261 tcatgatcag gtgtttcact gaataataac atcgccattt ggctgtttgg tcacagtaat
     4321 ttgctccaga ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc
     4381 gttaaccgca atgacatggc aacccgccgc caggccagaa agcacgccag cgggagcatc
     4441 ttccaccacc acacactcct gcggcgcaag ccccagcagc tgcgcaccta acagatacgc
     4501 atcaggttct ggttttccgc gcttcactcg ctcagcggtt acaaacacct ctggtgcggg
     4561 aagcccagct attttatggc gcgctcgcgc taccggcatg gagccagaag tcacaatggc
     4621 ccacggaata cctgctttat tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt
     4681 aataccttcg gtttccgtgg cctcgatgtg ctccagacgc gtaaactcgg cggcaatatc
     4741 agcctcggat ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt taccgtgaat
     4801 gaaagccagc acctcttccg gcgctaaccc atgacgtctg gcccagttgc tccacgcccg
     4861 ttctaccgca ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa aacctttgca
     4921 ccgcacgcgg gcctcctcag gcgttgataa tttgattaat ttcgttggcg ctcaaatggt
     4981 actgacgcgg gcaggcatgc cacacattaa gcatgcgctg atatttttcc cacattggcg
     5041 tctgggcgtt aaaaccgtga gttccggcgt caaaatgggt atagcgccct tccacattaa
     5101 ccataaagcg gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg
     5161 tgacacgacg ttcatcgatg gattgctggt cctgcaaatt agaccaggaa acatgcaacg
     5221 catgatacat ctccataatg tcgatgatag tgcggcaggt ttcttctttc agctcgccaa
     5281 actcgcgatc caattcacgc atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc
     5341 gacggtaacg ttcagcattt gccggatcga gcatagtcat catcttgtac tgattagaca
     5401 aaataagacg ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact atactacggt
     5461 gaaaaaaaag aaggctgagt atgccttctt ttatatgcgt aatcaggggt caattacaaa
     5521 tcatcaagga aagttttatc cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc
     5581 tggtaatcag gcttgccttc aacgggtgcc aacacctgtc cagactcctg caatttaccg
     5641 cgaacttcat aaaaccagtt aaggattgca gggggtaatg gcgttacaga acgcttgccc
     5701 agccaccaca atccctgcat gggtaaactt aaggcgaaca gcgcagtggc aactgccggc
     5761 ccaagctgac cgcccagggc aatctgccag cagagagtaa atacggcgat cggcggcata
     5821 aaacggatcg cataacgcgt catcttgata acgcgatttt cgacaaagac cggggcaagg
     5881 cgtttttcca gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa
     5941 ttaacagaac gattatccgg cgttgacatg cttcacctca acttcacata taaagattca
     6001 aaaatttgtg caaattcaca actcagcggg acaacgttca aaacattttg tcttccatac
     6061 ccactatcag gtatccttta gcagcctgaa ggcctaagta gtacatattc attgagtcgt
     6121 caaattcata tacattatgc cattggctga aaattacgca aaatggcata gactcaagat
     6181 atttcttcca tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt
     6241 catcatgcgc tacgctctat ggctccctga cgttttttta gccacgtatc aattataggt
     6301 acttccatgt cgagtaagtt agtactggtt ctgaactgcg gtagttcttc actgaaattt
     6361 gccatcatcg atgcagtaaa tggtgaagag tacctttctg gtttagccga atgtttccac
     6421 ctgcccgaag cacgtatcaa atggaaaatg gacggcaata aacaggaagc ggctttaggt
     6481 gcaggcgccg ctcacagcga agcgctcaac tttatcgtta atactattct ggcacaaaaa
     6541 ccagaactgt ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag
     6601 tataccagct ccgtagtgat cgatgagtct gttattcagg gtatcaaaga tgcagcttct
     6661 tttgcaccgc tgcacaaccc ggctcacctg atcggtatcg aagaagctct gaaatctttc
     6721 ccacagctga aagacaaaaa cgttgctgta tttgacaccg cgttccacca gactatgccg
     6781 gaagagtctt acctctacgc cctgccgtac aacctgtaca aagagcacgg catccgtcgt
     6841 tacggcgcgc acggcaccag ccacttctat gtaacccagg aagcggcaaa aatgctgaac
     6901 aaaccggtag aagaactgaa catcatcacc tgccacctgg gcaacggtgg ttccgtttct
     6961 gctatccgca acggtaaatg cgttgacacc tctatgggcc tgaccccgct ggaaggtctg
     7021 gtcatgggta cccgttctgg tgatatcgat ccggcgatca tcttccacct gcacgacacc
     7081 ctgggcatga gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg cctgctgggt
     7141 ctgaccgaag tgaccagcga ctgccgctat gttgaagaca actacgcgac gaaagaagac
     7201 gcgaagcgcg caatggacgt ttactgccac cgcctggcga aatacatcgg tgcctacact
     7261 gcgctgatgg atggtcgtct ggacgctgtt gtattcactg gtggtatcgg tgaaaatgcc
     7321 gcgatggttc gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga agttgatcat
     7381 gaacgcaacc tggctgcacg tttcggcaaa tctggtttca tcaacaaaga aggtacccgt
     7441 cctgcggtgg ttatcccaac caacgaagaa ctggttatcg cgcaagacgc gagccgcctg
     7501 actgcctgat ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg
     7561 gcggtaacga aagaggataa accgtgtccc gtattattat gctgatccct accggaacca
     7621 gcgtcggtct gaccagcgtc agccttggcg tgatccgtgc aatggaacgc aaaggcgttc
     7681 gtctgagcgt tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga
     7741 ctacgactat cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg ctgaaaatga
     7801 gctacgttga aggtctgctt tccagcaatc agaaagatgt gctgatggaa gagatcgtcg
     7861 caaactacca cgctaacacc aaagacgctg aagtcgttct ggttgaaggt ctggtcccga
     7921 cacgtaagca ccagtttgcc cagtctctga actacgaaat cgctaaaacg ctgaatgcgg
     7981 aaatcgtctt cgttatgtct cagggcactg acaccccgga acagctgaaa gagcgtatcg
     8041 aactgacccg caacagcttc ggcggtgcca aaaacaccaa catcaccggc gttatcgtca
     8101 acaaactgaa cgcaccggtt gatgaacagg gtcgtactcg cccagatctg tccgagattt
     8161 tcgacgactc ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg caagaatcca
     8221 gcccgctgcc ggttctcggc gctgtgccgt ggagctttga cctgatcgcg actcgtgcga
     8281 tcgatatggc tcgccacctg aatgcgacca tcatcaacga aggcgacatc aatactcgcc
     8341 gcgttaaatc cgtcactttc tgcgcacgca gcattccgca catgctggag cacttccgtg
     8401 ccggttctct gctggtgact tccgcagacc gtcctgacgt gctggtggcc gcttgcctgg
     8461 cagccatgaa cggcgtagaa atcggtgccc tgctgctgac tggcggctac gaaatggacg
     8521 cgcgcatttc taaactgtgc gaacgtgctt tcgctaccgg cctgccggta tttatggtga
     8581 acaccaacac ctggcagacc tctctgagcc tgcagagctt caacctggaa gttccggttg
     8641 acgatcacga gcgtatcgag aaagttcagg aatacgttgc taactacatc aacgctgact
     8701 ggatcgaatc tctgactgcc acttctgagc gcagccgtcg tctgtctccg cctgcgttcc
     8761 gttatcagct gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg ccggaaggtg
     8821 acgaaccgcg taccgttaaa gcagccgcta tctgtgctga acgtggtatc gcaacttgcg
     8881 tactgctggg taatccggca gagatcaacc gtgttgcagc gtctcagggt gtagaactgg
     8941 gtgcagggat tgaaatcgtt gatccagaag tggttcgcga aagctatgtt ggtcgtctgg
     9001 tcgaactgcg taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca
     9061 acgtggtgct cggtacgctg atgctggaac aggatgaagt tgatggtctg gtttccggtg
     9121 ctgttcacac taccgcaaac accatccgtc cgccgctgca gctgatcaaa actgcaccgg
     9181 gcagctccct ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg
     9241 gtgactgtgc gatcaacccg gatccgaccg ctgaacagct ggcagaaatc gcgattcagt
     9301 ccgctgattc cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca
     9361 ccggtacttc tggtgcaggt agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc
     9421 aggaaaaacg tcctgacctg atgatcgacg gtccgctgca gtacgacgct gcggtaatgg
     9481 ctgacgttgc gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct accgtgttca
     9541 tcttcccgga tctgaacacc ggtaacacca cctacaaagc ggtacagcgt tctgccgacc
     9601 tgatctccat cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg
     9661 gcgcactggt tgacgatatc gtctacacca tcgcgctgac tgcgattcag tctgcacagc
     9721 agcagtaatc tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc ggaaataatc
     9781 actatttccg gttttttatt ctcttaatct gcattaatcc tttctgatta tcttgcttaa
     9841 ctgcgctgca tcaatgaatt gcgccatttc actttgcata cttaccactt tgttttgtgc
     9901 aagggaatat ttgcgctatg tccgcaacca ctgaatccaa accaacaaga agatgggcaa
     9961 tgcccgatac gttggtgatt atcttttttg ttgctatttt aaccagcctt gccacctggg
    10021 tagttccggt ggggatgttt gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa
    10081 aaacacgcaa agtcgtagat ccacactcat ttcgcattct gactaacgaa gcaggcgaac
    10141 ctgagtatca ccgcgtacag ctgttcacga cgggcgatga acgcccgggc ctgatgaact
    10201 tcccgtttga aggattaacc tcaggatcga aatacgggac agctgttggc atcatcatgt
    10261 ttatgctggt gattggcggc gcgtttggca ttgtgatgcg tacaggaacc attgataacg
    10321 gtatcctggc gcttattcgc catacccgcg ggaatgaaat tctctttatt cctgcgctgt
    10381 ttattctgtt ttcacttggc ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg
    10441 ccattatcat cgcaccgcta atggtccggc tgggctatga cagtattacc accgtcctgg
    10501 tgacctatat tgccacgcaa atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg
    10561 tcgttgctca ggggattgcc ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg
    10621 tgtgggttat cgccactctg attggcctga tctttaccat ggtgtacgcc tcacgagtga
    10681 aaaagaatcc tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg
    10741 ctgatgttga acaacgtccg tttacctttg gtgactggct ggtattgatt gtcctgaccg
    10801 ccgtaatggt ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt ccagaaattg
    10861 ccagccagtt cttcaccatg ggtctggtga ttggcatcat cggcgttgtt ttccgcctta
    10921 acggcatgac ggttaatacc atggcttcat cctttaccga aggggcgcga atgatgatcg
    10981 cccctgccct gctggtgggt ttcgccaaag ggattttgct gctggtcggt aatggtgaag
    11041 caggtgatgc cagcgtgtta aataccatcc tcaacagcat tgccaatgcc attagcggtc
    11101 tggacaacgc tgtcgcggcc tggtttatgt tgctcttcca ggcggtattt aatttctttg
    11161 tgacatccgg ttctggtcag gcggcgttaa ccatgccgtt actggcaccg cttggcgatc
    11221 tggtcggtgt taaccgtcag gttaccgtgc tggcattcca gtttggtgat ggcttcagcc
    11281 acatcattta cccaacctca gcttcgttaa tggcgacgct cggtgtttgc cgggtggact
    11341 tccgtaactg gctgaaggtg ggcgcgacac tgcttggact gctgtttatt atgtccagcg
    11401 tcgtggtgat cggcgctcag ttgatgggct accactaaaa atgttaagag ccgcaattgc
    11461 ggctcttttt tcattctgcc gtttcagtct ctacggcttc atttttggca ttgcgcttca
    11521 tccacaacgc cagcgctttc agcgagtctg gagtgaactc atcgcagcgt gcggtgattt
    11581 cttccggcgt cagccagcaa acttcactga cttcatcttc ctgtagggcg aagggaccgt
    11641 gagagacgca gctgaacaat gcgccccaga cacggcaatt tttatcttcg aaatagaact
    11701 gcccgtgctc agcaaagggg acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg
    11761 cggattccag cagttgctca tcggcctgga ccacaccgcc tgcggtcgca tctaacatgc
    11821 cgggtaaaaa gtcttttgtc tcggtacgac gctggaccag aattttgccc atgccatcat
    11881 gcacgacgat gtaagttgca cgatgacgca gacactgtgc ccgcatttgt tcccggctgg
    11941 cttgtgcaat gacttcgttc tcttcattga caatatccac ccattcagta cttgccaaac
    12001 gacgctgttc cattatcagg aaaccttctt tttctggcgc tcttacggcg cattttgagt
    12061 tgtgggtaaa ttacggatta atcgcgacct gcgcaatgat actttgatca ttgagtgcga
    12121 ttacactaag tacgtcatta tccagcatgc cataactcgc cggattaccg cctttcggaa
    12181 gactcaccga gccggggttg aagtggaaaa tctccccccg ctgttctgcc actggcagat
    12241 gggtatggcc gtacaccagc acatcgttct ggtttaaagc aggcagattt tccgggccaa
    12301 aaagatgacc atgcgtcaaa aacagacgtt gtttttccag taatacctgt tgccacggcg
    12361 cggttatcgg gaaatgcagc agcatttgat ccacttcgct gtcgcagttg ccgcgcacag
    12421 cgataacctt atgtgccact tcattaagcc gttcagcgac tttggctggc gcgtaaccct
    12481 ccggtaaagc attacgcggg ccgtgattca acacgtcgcc aaggatcacc agccactggg
    12541 caccgctttg ggcaaacaac tccagaacac gttccgtcgc cggtaacgac ccatgaatgt
    12601 ccgatgcaaa catcagtttc atcactcact cctcgtcgaa aaaacgtaag ctatgatact
    12661 ggattctgtc gttgctatca gccagattgc ttcgccgaaa gtgcaataaa acgctggaca
    12721 gacgctcgct gccactggaa tgtcgcataa tccaccaggc gttctggcac ctcatcgcca
    12781 tgtagtacca ggcggttaat cattagcgcc agatcagtat cagcaatgca ccattcacca
    12841 aataaattcg gctgacccag tactaacaaa tgttctgcca tcgcaaacag tttctctgca
    12901 ctggcttttc cctcggccgt tagtggcgct tttttcgccc ccgcaaagac aacatccgtc
    12961 ggacgctctt cgcggatggg catcagatcg ctgcgcagcc aggcctgaat ctgtcgtgca
    13021 cgcgcacgat tttctaaatc aagcggataa atacgttccc aggtcggtgg cgcaaatcga
    13081 tcttccagat actccgcaat ggcagaagat tcactcaact caaaatcatc gatttgtaat
    13141 aacggcacac ggcgtgtctg accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg
    13201 tcgaggtcga tggtcttgat atgaaacgac aggccttttt cctgcaacgc cacccaggcg
    13261 gataacacat aaggggagaa aaagtgggca tctgaccaaa gcgtgatagc gggtttactc
    13321 attatgtcct cggcaataaa gggggctggc tatcaaacta tagagccttt ttgtcactgt
    13381 cacgtgacga atactcacgt aagtcgccgc gctttctata cttgtggtgt tcagatgatt
    13441 aaacaaccgg agctgcaatg atcgatctct atttcgcccc gacacccaat ggtcacaaaa
    13501 ttacgctgtt tctcgaagaa gcagagcttg attatcgctt gattaaggta gacctgggga
    13561 aaggcggtca gtttcgtccg gaatttttgc gcatttcgcc caacaacaaa attccggcaa
    13621 ttgttgatca ttctcctgcc gatggcggcg aaccgttaag cctctttgag tctggtgcca
    13681 ttttgttgta tctggctgag aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg
    13741 ccgccacatt acagtggtta ttctggcagg taggcggact ggggccgatg cttgggcaaa
    13801 atcatcattt taatcacgca gccccccaaa ccattcctta cgctattgaa cgttatcagg
    13861 ttgaaactca gcgtctttac catgtactga acaagcggct ggaaaactcg ccctggctgg
    13921 gaggcgagaa ctacagcatt gcggatattg cctgctggcc gtgggttaat gcctggactc
    13981 gccagcgaat tgacctcgca atgtatccgg cagtcaagaa ctggcatgag cggatccgtt
    14041 cgcgccctgc caccgggcag gcactgctaa aagcacaact cggtgatgag cgttcggata
    14101 gttaacagaa acaggttctc gtgtattatt tcatcctaag taaaacaacg gagaacctgc
    14161 aatggcacaa cctgccgcta ttattcgtat aaagaacctt cgtttgcgta cgtttatcgg
    14221 aattaaggaa gaagaaatta acaaccgtca ggatattgtt atcaatgtga cgatccacta
    14281 ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat gcgctgaatt atcgcaccgt
    14341 aacgaaaaac attattcagc atgtagagaa taaccgtttc tctttgctgg aaaaattaac
    14401 tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg acgtatgctg aagtggagat
    14461 cgataaactg cacgcgctgc gctacgccga ttcggtatcc atgaccttaa gctggcagcg
    14521 ttaaccgcca tatcgggagg ctgcatgaac atagtgatca ccggagggac gggattaatt
    14581 ggtcgccatt tgattccgcg tttgctggag ctgggccatc aaatcacggt agtgacgcgt
    14641 aacccacaga aagccagttc cgttctcggc cctcgggtga cgctatggca agggctagcc
    14701 gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc tggccggaga accgattgct
    14761 gataaacgct ggactcacga gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg
    14821 caaaaactgg tcgatttgat taatgccagc gacacgccgc cgtcggtact catttccggc
    14881 tcggcaacgg gctattatgg cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg
    14941 cataacgaat ttactcataa actctgcgcc cgctgggaag agattgcctg ccgggcgcaa
    15001 agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg tactggcacc ggacggcggt
    15061 attctcggta aaatgctgcc accgtttcgt cttggcctgg gcgggccgat tggttccggt
    15121 cggcagtatc tggcctggat tcatattgat gatatggtca acggcattct ctggctgctg
    15181 gataacgacc tgcgcgggcc atttaatatg gtttcgccct acccggtacg caatgaacaa
    15241 tttgcccatg cgctcggtca agcgctgcat cgcccggcca ttttgcgcgt ccccgcaacc
    15301 gccattcggc tgttaatggg cgaatcttca atactggtat taggtggaca acgcgcgctg
    15361 cctaaacggc tggaagaagc gggttttgcg tttcgctggt acgatttaga agaggcgctg
    15421 gcggatgtcg ttcgctgatg tggtttacag caaacatccg ccagttaact cccggtgtta
    15481 caggattagt ggctttgcgc gataagatcg tctggtgaaa gtcgggtcac catcataact
    15541 aactctctgt ctaaacctct atccagcatc tcctgagcaa tacgcagggc ttcttcgtgt
    15601 ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa tggtcatcag tttctccttt
    15661 tcttgtggtg cgcgttctgt tatctcacca ataaatgcac ggaaacgctg ggcatcccct
    15721 gtttgtaata cgtaattaaa cagggctttt agctgtctgt cattagtgtt ccctgtaact
    15781 agcagcgaaa caatttggtc gactaatccc aacagatcgc gctgacgaat atgtttctga
    15841 attaactcca acagcgccat tttgcggtgt tgcataatct cgtcatccgg caccacggta
    15901 atatccacca acggaaacgc tgatgaatat attttgcggg ctatagcagg ctcggcaaat
    15961 tcatcaagcc agcagagtga ataaggataa ggacttctgc aaccatgata aaacagcatc
    16021 gggatcacca atggaagctc tttatagccc gcatcaagat ggttttgcat tgccgcaatg
    16081 gaataacgca tcatgcgaaa agccattaat tcttccggct tactttggtg ctctatcact
    16141 acataaatat aacccactcc ctcctgcgtt ttcacagacc acaagaggtc ggaataatat
    16201 tgccgcaggt cgtcatcaat aaaactgttt ggttccagtt taagcgtcgt taaatcacac
    16261 agtttgcgca gcggcgcggg aagatgaata tcaataaaat cccgcgcggt gtctggatgg
    16321 cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag ttgtcgatat tgtcatggcg
    16381 ttccgtcacc cctcaaattt aagatgacgc gacaataacc acgattaaca cccaatgcag
    16441 caggcagttt ttatctttgg acagcgaatt cagaagtatt tttagcggac tgcaactttt
    16501 acatttcagc ggaagctgac tcacaaattc cccatgaaaa tactccggca gcgttagcca
    16561 ccggagagga aattatttca gcgatccctt aaggaactgt tgcagacgag ggctttgcgg
    16621 gttgccaaat aactgctccg gcgcgccctc ttcttctatt tttccctgat ggaggaaaat
    16681 gacgtgagta gaaacatggc gagcaaagcc catttcgtga gtcactacca ccatggtttt
    16741 cccctcttct gccagttgct gcataatacg taacacttcg cctaccagtt caggatcgag
    16801 cgccgaggta ggttcatcaa acagtaaaac ttccggttcc atcgccagcg cccgcgcgat
    16861 agaaacacgc tgttgctgac cgccggaaag atgcaccgga tatttcccct gcgcacgttc
    16921 gtctatcccg actttcgcca gatacttcac cgcccgctcg cgcgcttcct gcttgctcag
    16981 gcccaacacc tgaatcggcg cttccatgac gttttccagc accgtcatat ggctccagag
    17041 attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt aagcgcagtt gatttttatc
    17101 ggcgactttg agttgaccat ctttgtcgcg caccagattg atcgtctggc cattgaccac
    17161 gatcgaccct tcactcggtt tttcgaggaa gttaatgcag cgcagaaagg tacttttccc
    17221 cgatcccgac gatccgatga tgcttattac atctccggca ttcgcttgca gtgagacccc
    17281 tttcagcact tcatgttcgc cgtagcgttt gtgcaaatcg ataacgttta atttattctc
    17341 ggacatcgta ttctcagtgc gttgaagaag gtttcacatg ctgcaaccag cgtttttccg
    17401 ctctgcgaaa gaggctgatc aggacataag agatgattaa atagagcacc gcggcaatgc
    17461 cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc gcgggctatt ttcagcagat
    17521 ccggcacagt ggccgtaaac gccagcgcag tagagtgcag catcaggatc acttcgttgc
    17581 tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat aatgcaacga tacattttaa
    17641 aagtcgagaa gccataggct ctggcggctt caatttcccc atgcggaacc gaacggattg
    17701 ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag cgtcagcgcc agcacggtac
    17761 agttcaggcc actgcggaag aaagcgttaa ggaattcggt tcccttaaca atctcaagcg
    17821 tgtacatgcc ggaatagaac accagcaact gaacatacag cggcgtaccg cgaaaaatat
    17881 aggtaaataa ccagattgga aactggatgt atttattact ggagacacga ccaatcgcca
    17941 gaaacagcgc caggactccg cctatcacta ccgacaaaat aagcagccac agagtgatcg
    18001 ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg tttccagtat tcatgtaaga
    18061 tttcgatcac aggtcagccc tcttcacacc cacggagtag cggcgctcaa ggaacagcag
    18121 cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg ccacagacga tggcgaaata
    18181 gaacggttcc caggtacttt tgcctgccag ttgggtggct ttgaccacat cttccaggcc
    18241 gagtaacgaa accagtgcgg tagatttgag gatcacctgc cagttgttgc caatgcctgg
    18301 cagggcgtaa cgcatcatcg ccggaaacat gatccgccga aacacttgcc cacgagtaaa
    18361 accgaacgcc gtcgccgcct ctatatgccc tttcggcact gccataaacg cgccacggaa
    18421 cgtttcggta aaataagcac cgtagataaa accgagagta ataataccag cgaccatcgg
    18481 atcgatatca atctgcccga cgcccatcgc ctccgtcacc gtgtttagtg caatctgcaa
    18541 accgtagaaa atcagcagca tcaacaccaa gtccggcacg cctcgaatca gcgtggtgta
    18601 cccttcgaaa atcagccccg aaagccgatt ttgcgagagc ttaccgccag cgccaattaa
    18661 accgatgatt acagcgagca ctacagaact gatagccagc tccagcgtga cgagcgcacc
    18721 ctgtaaaata acacctgaaa acccatacaa catgctgcct gtcctgtcgt gtgtggtgaa
    18781 ttaccgcctc ttgtctccct ccggttaccc gaagggagcg ggctttcttt caccgatggc
    18841 gattaaccgc cataaacatc aaaatcgaag tactttttcg ctaatttctc gtaagtaccg
    18901 tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc gcagttcgtt atcttcttta
    18961 cgcaggccca tgccggtccc tacgccaaac agtttttcat ctttaacaga cgggccaccg
    19021 aatttgtaat ctttaccgac aggttgtttg aggaaacctt cgctggcagc gacctcatcc
    19081 tggaacgcgg catcaatacg tccggcagtc aggtcagaat aaatgttgtc ctgcccctga
    19141 tacgagacga tttcaatgcc ttttggtgcc caatgttcat taccgaacgt ctcctgggtg
    19201 gtgccctgca atacgcctac ccgtttgcct ttcagcgact cgactgtcgg ctgaatgtcg
    19261 gaatttttcg ccaccaccaa acgagaatcg gcagcgtaca gtttgtcggt gaaggctatt
    19321 tcttgctgac gtttttccgt aatggaaagc gatgacatga tggcgtcaat cttcttcgct
    19381 tttaaggacg ggattaacgc atccagcgga ttttcgacaa acgtacattg cgtattgatg
    19441 cgtttgcata attcctttgc cagatcgata tcgaagccaa ccagttcgcc ttgtgaattt
    19501 tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc ggatgttttg cggaatcgca
    19561 gcaaacgccg cagttgcgct ggagaaggcc agaaccagag agagcgatag caccagtttt
    19621 ttcataatgt cctcaactga cagtcttttt atagggaata tttacaggtt tgttacactt
    19681 atcgtgccat aaaattgacc atcaaggcaa aatatcccgc cccgttagac gtattatttt
    19741 tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga tagtgcaaca aacctggcgt
    19801 gcaccatgat gatcattcca tcaggtacag cttcccagcg acgtatcagt caccgtagac
    19861 attaaagtcg aaatactttt tcgccatctt gtcgtaggtg ccgtcctgac gcagctcgcc
    19921 aagcgcctta ttgaaggcag ccgtcagttc agcatcatct ttacgtagcc ctacaccggt
    19981 gccatcaccg aagtattttt tgtcttttac tgatgagcca gcaaaggcga aatctttacc
    20041 agcaggttgc ttgaggaatc cttcgctggc agcaacttca tcttgtaacg cagcatccag
    20101 acgtcctgca gccagatcgg aatagaccaa atcctggttg gcataggcca ccacatccac
    20161 gcctttgcta cgccaggtct cgttagcgta agcttcctgg gttgatccct gcagcacacc
    20221 aacatgttta cctttcagtg aatccagcgt tggctgaatc ggtgaacctt tggcagcaat
    20281 caaacgagaa tctgcggcgt acagcttgtc ggagaaggca atctcctgct gacgtttatc
    20341 ggtaatggaa agcgacgaaa taatggcgtc gatttttttc gctttcagtg aggggatcag
    20401 cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc atccgtttgc acatctcgtt
    20461 accgagatcg atatcaaagc caacaaaatc acctttagca tctttcgacg agaacggcgc
    20521 ataggtggta tcggttccga tacgtaccgt ctccggtagc gccgcatagc tggaagccgc
    20581 tgcggagaga ccgactaaca aagacagagc gagaatcgac ttcttcatac ataaccctca
    20641 agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg tttgcaggct ctttcatgca
    20701 ggtcttatgc catcttgacg acaacagtaa cattcaacgt taaatatgtt aataagacgt
    20761 tgcattattg tcctgaagtt gaagatagca ggtatagcgg ttgaatcgca gcgtttcgat
    20821 tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt gcaaaattgc cctgaaacag
    20881 ggcaacagcg gagttatgcg ccctgccagc gggcaaagag atcttcagga agggttattg
    20941 caaactggtc aagaacacga ttaaccgtct gatttatcac atcatcaagg gattgcgggc
    21001 gatgataaaa cgccggaacg ggaggcataa tcaccgcacc gatttctgcc gcctgagtca
    21061 ttaaacgcag atggcctaag tgcaatggtg tttcacgcac gcagagcacc aacgggcgac
    21121 gctctttcag caccacatct gccgcacggg tcagtaagcc atcagtatag ctatggacaa
    21181 tgccggaaag ggttttgatt gaacagggta aaatcaccat ccccagcgtc tggaaagaac
    21241 cggaagagat gctggcggaa atatcgcgcg catcgtgcgt gacatcagct aatgcctgca
    21301 cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg gcgcgccgcc tggctcatca
    21361 ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg taataagcgc acgccataaa
    21421 tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt cataaatagt tgccctgtca
    21481 gacttgcagg cagactttgc agtatttcgg cgggagttgc aagtcagggt gccagaccgg
    21541 caccctcagc gaaggcatca tccttcgtta tgcatttcga gattttccac ttcgttctga
    21601 cgttgcactg ctttggcgtc atcattacgt aacgtatcga ggaaatcgag gtagccctga
    21661 tcaacatctt tggtgacgta gacgccgttg aacaccgagc attcaaactg ctggatatcc
    21721 ggattttcag cgcgaacggc gtcgatcaga tcgttcagat cctggaaaat caacccgtca
    21781 gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt gagcgatcag ttccgtggcg
    21841 ctcggcatat caataccata aacgttcggg aagcgaattt ccggtgccgc agaagcgagg
    21901 tacactttct tcgctccggc ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca
    21961 cggacgatgg agtcgtcgac cagcaggacg tttttatcgc ggaactcggc gcggttggca
    22021 ttcagtttac ggcgcacgga cttacgacgc agctgctggc ccggcatgat aaaggtgcgg
    22081 ccaacatagc ggtttttaac gaagccctgg cggtacggtt tgcccagaat acgagcaatt
    22141 tccagcgcga tatcacacga ggtttctggg atcgggatca ccacgtcgat atccagatct
    22201 tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca tattcacacg cgcgctgtaa
    22261 acggaaattt tgtcgatgaa cgagtccggg cgagcaaagt atacatactc aaacaggcac
    22321 ggattgctga ccggattgtc agcacattga cgggtaaaca actgcccttc ttcagtgatg
    22381 taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa agcccagcgt atcgagcgct
    22441 acgctttcgg aagcgaccat atattctgta cggttctcgt caatatcacg ttttcccagt
    22501 accagcggac gaatcccgtt tggatcgcgg aaagcaacca taccgtggcc gataatcatc
    22561 gccacacagg catatgcgcc gcggattaag cggtttgtgg cagcaatggc agcgaaaata
    22621 ttgtcggctt ccagcgggta gtggcggaag ttgtccagct cgctagcgaa gatattaagc
    22681 agaatttccg agtcggaagt ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta
    22741 cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa gcgtaatgcc atacggagag
    22801 ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca
    22861 tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc
    22921 accagcccgt tcgctttacg caaacggaag cagttattgg catctatggt gatgatgccg
    22981 gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat cataaatcga ctggttaacc
    23041 ggcataacac cggcgatacc gacaataccg cacatacgtc tttttcctcg ttaagccaca
    23101 tctcagagca cttacgctct gggcaagaaa cttgacgagc tttgcagata atcaaaaaac
    23161 catctgatga taaaactgaa ttgcgggatc agctgtgatt tgctccagtc ttcgcttttc
    23221 gacaccccgg taaaggagtc gagaaagaag agaatggcgg caacaatcaa cacaccgcgc
    23281 aacgcaccga aacagacgcc cagcacccga tcggtgcctg acaaccccgt tttctccacc
    23341 aactggccta tcacgaagtt cacgatagca ccaacgatca gggtagcgat aaacagtacc
    23401 gcgatggcaa ttccatttcg aaccagttcg tcttcaaagc ccgtaaacca gactgacagg
    23461 taagtgtagt aatgactggc aacaaagaaa gcacaacccc atgtcaccag cgataacgct
    23521 tcacgaacaa agccgcggat caggctaacc agagaggaaa aagcaatcac cgcgattatg
    23581 gcgtaatcaa tccagaccat atgtgtccca cgattttacg ccctgtcatc ctgttcgggg
    23641 cgcattctaa cagaaaaaga aaacgtttgc gtagggattt ccttcccgcg catcaataaa
    23701 aatggcgctg aaaaaatatt caacgccatc gactttttat gcctttgcgg catcgtgcaa
    23761 tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg gttctaacac tgcccaggcc
    23821 tgataagacg cgccagcgtc gcatcaggca agactgtatt aattcggcgt atagcccatt
    23881 accacgccac ttaagccaga aagttgcttc aactcaccca gcgaaccttt cagcttatct
    23941 ttcgaggcat ccggcccaac cagaatacgg gtaattttac cctgcactgg cgtggatggc
    24001 gacgtataaa cccgataacc ggcaccgcgc agcttaccga caatctcatt cactttatcg
    24061 gcatttttca gcgcacccag ttgcacaaca taggctttac ccgttggcgc tgctttttct
    24121 tccacgaccg gcttcggttc tggcttcggc gcaggtggtg cttcaacctt cggtttaggc
    24181 ggctccaccg gtttcgactt cggtggggcg accggtgcag gttccggttc aaactcggtg
    24241 ttattggctg caatagtggt cggatcgagc gacggtgctg cggcatcacc tgcccgcacc
    24301 tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct gggtggcggc tggcatcata
    24361 tcaggctcat cgcgatcgcc cgctttcggc accagcggga tagccgcgaa ctcatcctga
    24421 taatgttttt tctgcccgtc cagcagccct ggaagtacaa tcacccccag cgccaccagc
    24481 acgatcgtgc ccactaaccg attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat
    24541 cacttccatg acatgtgcga cagtgtggaa agaaccacac accagcacgg tgtcttccgc
    24601 tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt
    24661 acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca
    24721 ataccagtca tcaaccacgc ttttcaacca ggccagagtt ccggcaatat ctttatcatg
    24781 tagcataccg ataaccgcca gcacgcgccc gtttttcggt agcgctttca tacgcccggt
    24841 gagatattcc gccgcatgtg gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct
    24901 cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc
    24961 actgacttcc agcccgctgg cacgcagtgc cgccagcgcc gttgcggcat tcggttgcgg
    25021 gacaagcggc aacggcagat tttccagcgt gccgtgagca tcgctaaacg cccagtcatg
    25081 atcggtgacg gaatagttcc actcaacgcc ccgacgttgt aacagtgcac ctttttcctg
    25141 tgccacatca gcaatggtag aaggcatttc cggctcaccg acaattgccg gtttttcgct
    25201 gcggaagatg cctgctttct cgcgaccaat actttcgcga tctggaccca gccagtcggt
    25261 atgatccagc gcaatactgg ttactaccgc gacatcggca tcgacaatat tggttgcgtc
    25321 cagacgaccg cccagcccta cttccagaat caccacgtca agttgtgcct gcttgaacag
    25381 ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga
    25441 ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc aattcctggc cctgcacacg
    25501 tacgcgctcg gtataacgca ccagatgcgg tgaactgtag acgcccactt tgtaccctgc
    25561 cgccatcaga atcgactcca gcgtacggca ggtggtgcct ttgccattcg tacccgcaac
    25621 ggtaaacaca aatggcgctg gtttcagaac gccaagacgc gccgcgacct ggctcacgcg
    25681 ctcaaggccg agatcgatag ttttactgtg caggttttcc agataagaaa gccacgaagc
    25741 cagaggcgac gcggcttgag gagtgcgttt gataatcatg gtatccgctg attcgttacg
    25801 gtgagaatag caaaagggca gagccagtgg ccctgccctt atcagttatc aggcctcagg
    25861 ttcctgatcc ggtaccgggg gtaccactac gccttcacgc ggcgcttcag gattcggcgc
    25921 tggcagattc atcaacttcg ccagaatgct cgccagtttc aggcgcattt ccggacgacg
    25981 gacgatcatg tcgatcgcgc ctttctcgat caggaattca ctgcgctgga atcctggcgg
    26041 cagtttttcg cgaacggtct gttcgataac acgcggaccg gcaaagccga ttaacgcttt
    26101 cggttcagcg atgttgagat cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt
    26161 cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc
    26221 agaggttttc gccatctgca tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga
    26281 ggcggagaag cagatcagcg ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa
    26341 acgtgcaccc acaacagacc ccattgaacc gcccataaag gcgaactcga atgccgcagc
    26401 gacaaccggc attccataca gagtgccttt catcaccacc agcgcatctt tttcgccagt
    26461 ttctttctgc gcagatgcca gacggtcttt atacttcttg gagtcacgaa acttcagcac
    26521 atctttcggc tcaagctcgc tacccagctc cacaaggctt ccttcatcta acaggctatg
    26581 caggcgatta cgcgctgtca tacgcatgtg atggtcacac ttcggacaga cctcaagatt
    26641 acgttccagc tcagcgcggt ataaaacctg accgcagcta tcacacttag tccacacccc
    26701 ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt ttaattcgtt caatccagct
    26761 cattagggac ctttctgtct gaacctggtt cgatgccagt tttatctttg gggacgcata
    26821 atgccatttt tgcccccaac agaccatgaa tgttgcacat taaaacataa cagcccgaaa
    26881 ctttggataa aaaagtggtc gaaccgcgga gttacttttt attttgcggc gcgtgccgca
    26941 gcgcgtttgt gacgaataaa ttcgaaaacc ccaggcaata ttgaaacaaa aataatgcca
    27001 acaatcatca gcttaaggtt gctctgaatg aaggggagcg tgccgaagaa ataacccgcg
    27061 taggtaaaaa tcagtaccca caacagtgcg ccgatcacgt tataagcggc gaaatgacgg
    27121 tacgacatgt ggcccattcc cgcaacaaac ggcgcgaacg ttctgacgat cggtacaaaa
    27181 cgggcgagaa taatcgtttt gccgccatgt ctctcgtaaa actgatgagt cttatcaaga
    27241 taactgcgac gaaagatttt cgaatccggg ttactgaaca gtttgtcacc aaataaccgt
    27301 ccaatggtat agttcactgc atcaccgaca atagcggcaa tcaacataag caccaccatc
    27361 atatggacat tgagatcgtt agtttccagc gacgccaatg caccagcaac aaacagcagc
    27421 gaatcaccag gcaggaatgg cgttaccacc agccccgttt cgcagaacag aatcaaaaac
    27481 agaatggcat aaacccagac gccgtactcc gcgaccagct ctgccagatg cacgtcaatg
    27541 tgcaggataa aatcgatgag gaaataaatc aggtccatat ttgcctatgc cttgtactcg
    27601 tcatctttca ggctgtaact acgttggcta cagcctgaaa tgttccgagc attattctcg
    27661 ttagtccgcc agaaatagcg ggcccattgg cggttttgga agatcatacc ggtcagggta
    27721 atccaccgcg accagataca gcccttccgc ttttgccgtt gctgccgccc gcgttctgtc
    27781 ctttgccgcc agcaactctg ctatccagct ctccggctgg ttgtgggcac cgacttccat
    27841 caggctgccg acaatattcc tgaccatatg atgtacaaag gcattcgctt tgatatctac
    27901 caccacataa ggaccgtgac gcatgacatt aatatgcata acgttgcgcc acggggttcg
    27961 ggactggcac tgcaccgcac ggaacgaggt gaaatcattc tcgcccagca agcattgcgc
    28021 agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag
    28081 taccgccggg cgcagccgat gattgtagat gatgtagcga taacggcgag ccgtggcgct
    28141 aaatcgggca tgaaaatcat caggtacagc tttaacccaa cgcacggcga tgtcaccagg
    28201 taaattcgca tttacgccca aggtccacgc cgcgtctttg cgctgcgcgg ttgtttcgaa
    28261 atgcaccact tgcccggtac cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt
    28321 gatgggttcg ttcgccacct gggagagcgc cttttccagc ttctcctgca cactgcggac
    28381 ttcattctgc cgttgccagc cgtaatactt actgccgtcg tactcaatgc ccagcgcaat
    28441 tttataaact ggcagttgtt gctggtcgga cattagtaca gatactcctg caccagtttc
    28501 tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag
    28561 aactggactt gctccggcat accgtagtca ttacgcacgc agccaacaga aagatgcggc
    28621 gtacccgaag catcccctac ctgagtcggg aattcgttct cttcagagag cacaatatct
    28681 tcgccctgag caaacgcatc acgcgcttct tctgccgcca gcggacgcag tgcttcaaag
    28741 ttgaccatct gggcatgacc gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc
    28801 agcccttcgt cctgcaggat tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg
    28861 ctaccttcgc tatccggcaa caacggcagc atattgaacg ccagttgacg accgaagaaa
    28921 tcttcttcgt caatcggaat gccgttgagc aatttcgcac tctgccccgc taacgcatcg
    28981 accgcttttt tgccctgggc ggaggctgaa atcaggctgg taacactgat acgcgacaaa
    29041 ccgccctgat cgattaacgg tttcagtgcc gccagcagct ggctggtcag actgtctggt
    29101 acggcgatga cattccggtt gcggtaatcg gtcagtacaa acgggtttac ttccggcacc
    29161 accagcggta cgtcgggttc gagagcaaac agtccgctgc tgtcgatcac caggcaacct
    29221 gagttggtcg cttcttcaac ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc
    29281 agctgcgcct gcgtccagtc gaattcagcg gcatcctgca cggtgattgt tttaccacca
    29341 aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg cataaatttc cccaaccggg
    29401 aactgacgtt cagccagcgt ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg
    29461 acggcaatgt tccagccttc agacatggtg gtttactcca gaaatagcaa agctccctgc
    29521 caatgttaca gcagagagca tgaagaagag attaacgtgc cggatgatga acggcgttaa
    29581 aacccagttt acacagcaat gatgccgcac tggcgtcatc acaaattaca tacagagatg
    29641 accattcacg gcgctcaaga tagtttttgc gcagtttatc gaactcaccc ggtatcccgg
    29701 cgactttacg cagcggtgcg tcatcgcggc gcacatcata caccaaatgc accagccttt
    29761 tcagcgtcgg ttgatcgagc gggccatgca gcgtaatgcg accaaactct ggcgcgggca
    29821 gtaatgtatc cagcgcaacg tgctgttcat gcccaataaa cttgctatag gcttcaaaca
    29881 cttgcgtagt accgcgtgct ttaccttcca gggtataacc tgcgatatgc ggcgtgccga
    29941 tatccacttt tgtcagcaac tccacgttaa gttccggttc gccttcccag acatccagca
    30001 ctacgcttaa cttctggcct tcattcaggc aggtcagcaa cgcagtatta tcgacgactg
    30061 cgccacggca ggcgttaatc agaatcgctc ccggcttcag gctacggatc agtttttcat
    30121 ccgccagatg cagcgttttg tacggcccat ctttgaagag tggcgtatgg aaagtcagaa
    30181 tatccgcatg ctgaactaat tcatccagcg agcggaaatc accctcatcc ccacggtcag
    30241 cgcgaggcgg atcgcaaagc aatgttttaa tccctaacgc ttccagtcgc gcctgcaatc
    30301 gacgtccaac gttaccaacg cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat
    30361 cgcgttcggc aagcatcagt agtgaggaga aaacatattc caccaccgca atcgcattac
    30421 agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa ccatgcttca tcgacatggt
    30481 cggtccccgc agtggcagtg ccaacaaatt taatgggttt tcctgccagc aaagattcat
    30541 tcactttcgt gaccgaacgc accatcagcg catccgcgtc tgccagttga gcaacgggga
    30601 ttggacgccc gggaaccgcg gtcacctcac ccaaacggct aaataagtcg cgggcataag
    30661 gcatattttc atcaacaagg attttcacgt ttgtgttacc tgtatgagac gagagttaac
    30721 cggacaagtg tgccataatc tcgcggccag gcatacttgc gaagatttca ggtataagga
    30781 tacgtaatga tacaacctat ttccggccct cctcctgggc aaccaccagg tcagggagat
    30841 aacctgccgt ctggcgcggg caatcagcct ttatccagtc agcaacgtac ttcgctggaa
    30901 agcttaatga cgaaagtgac ctcactgacg caacaacaaa gagcagaact gtgggcgggt
    30961 atcaggcacg atattggtct gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc
    31021 gctgagcata atctggcgca acgtctgctg gccgcgcaaa aaagccattc tgcccgccag
    31081 cttttagcgc aattagggga gtatttacgt ctggggaata atcgtcaggc ggtcacggat
    31141 tatatccgtc ataactttgg tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa
    31201 accattctca ccctgttgca ggaagggaag atggttattc cgcaaccaca gcagcgcgag
    31261 gcgaccgacc gtcctttatt accggcggag cacaatgcgc taaaacagct ggtgaccaaa
    31321 cttgcggcgg caacggggga acccagcaaa cagatctggc aatcgatgct ggaactttcc
    31381 ggggtgaaag atggcgagtt aattccagcg aaactgttta accatctggt gacctggttg
    31441 caggcgcgcc agacgttaag ccagcaaaat acgccgacgc tggaatcact acagatggcg
    31501 ctaaaacaac ctttagatgc cagtgaactg gcggcgttat cggcatatat ccagcaaaaa
    31561 tatggccttt ctgcgcaatc atcgctttct tctgcccagg ccgaggatat tcttaatcag
    31621 ctttatcaac ggcgggttaa agggattgat ccgcgtgata tgcaaccgct gcttaatcct
    31681 tttccaccga tgatggacac gttgcaaaat atggcaacgc gtcccgcgct gtggatactg
    31741 ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt agccccgacg aaatgacagt
    31801 atcgtgacaa taattcccag caccgcagag atcgccccgg caagaaatac cgaagagtaa
    31861 ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga cgccgagggc gatatcctga
    31921 aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc
    31981 tccacgccca gcgcaggaaa gataagcgaa catccggctc cggttaacgc cgcgcccgct
    32041 aatgcgaccc acgcacctgg ggcttgccag agcagcaaca agcccaccgt ttctacaagc
    32101 agagagacaa tcgccacttt cacgccgcca aaacggtccg gcatccagcc aaacatgacg
    32161 cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa agcccgccat cgcccatcct
    32221 ttgctggcaa agtagagcga aacgaaagtc ccgataaccg caaaaccaac gccttgtagt
    32281 gccagcccta accctggttt ccagataaac ccgacaacgc tccacagcga tggacgttct
    32341 cccgccaggg ccggtacttt gcgcactgtg ccgttacagg cccacgccag taagggtaat
    32401 accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc tatgaatcaa caggccaagc
    32461 ggagcaccaa cagcgagggc accgtaaatc gccattccat tccatgacat cactttgcca
    32521 gagtgttttg gccctacgat gcctaacccc caggtcagag cgcctgtcag taactggctt
    32581 tcaccaaacc caagaatcaa acgcccgatg accaacaggg caaatttgaa cggtgcggag
    32641 acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca gaccacaagc taacatcccc
    32701 tgaagcgccg aacgttttgc accatattga tcggccagtc gcccggcata gccacgcgtc
    32761 agcaccgtag ccagaaactg aatcccgacg gcaatcccga ccatggtatt gccatagccc
    32821 agatcatgat gaacaaacag cgggataacc ggcaacggca gccctacggt catgtaggtg
    32881 agaaaaaccg caaaagcgat gcggaagagc gaaaaattgg cagaagatcg tgtttcggtt
    32941 tggcttacag cagtcatgca ttactccaga atgcagcgca aggcgaggag tatccccgtc
    33001 tcatctctct ggtttcaggg ttacggtgcg ttggcaggat ttaacgcgta cgtcttttca
    33061 gaaggaaatc gacaaagcgg gaagtttgcc tggaacaggc ggcgattgtc aatgatgtga
    33121 aaaagggaac catcaggttc ccttttgcgt tagtgccgga ggccgcatta gcgcgtaacg
    33181 tcggatgcga cgctggcgcg tcttatccga cctactccga ctactccggc ctactgcgaa
    33241 ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt
    33301 agacataacg gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca gacctgcagc
    33361 ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt ccagcatcag
    33421 cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg tcatggcttt
    33481 ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg ccagttcttt
    33541 cacgtcgcca accggagtcg aagtaccatg agagttcagg taatcgattg gggtatcaac
    33601 gccgtgcatc gccatcttca tgcagcgtac tgcgccttcg ccagacggag caaccatgtc
    33661 tgcaccatca gaggttgcgc cgtagccaac gatttcagca tagatgtgag caccacgcgc
    33721 cagcgcgtgt tccagctctt caaccactac cataccgccg ccgccagcga taacgaaacc
    33781 gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt atttagtaga
    33841 cagcgcaccc atcgcgtcga attcgcaggc catttcccag cacagctctt cgccgccgcc
    33901 agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac cgatacagtg
    33961 tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt taaacggcgt
    34021 ggcgaggcag gcagaaacgc cggatgccat cgctttggtg accacatacg ggccaaccgc
    34081 tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac gcggggaacc
    34141 gccgccagaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt ccggagaaag
    34201 gcccgcatct gcgattgcct gctccataga aaggaatgca taaatggatg cgtcgctcat
    34261 aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt tgccccagac
    34321 gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg aacgtccttc
    34381 acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa tgcccaggcc
    34441 agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa
    34501 tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccatttaa tagagaaatt
    34561 tgcgcagcct tacacacatc gctaagatcg agccaccgcc tgtaagacga gtaacttacg
    34621 tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt acacctgttt
    34681 cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag acgcgttatg
    34741 tttttctggg aggcaaccaa ttagaggtac gctttcctga gcatccacat cctctgtttg
    34801 tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg caggcattta
    34861 atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg cttacatttc attagttttg
    34921 agaaatttcc cctcacccgt gcggatttag ccttagcgca tcaacactgg ccggaactgg
    34981 ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt
    35041 tattgctcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt aacgaactga
    35101 ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg gacggctttg
    35161 cgccagcgaa aaacccggat atgtggacgc aaaatctgtt taacgccatg gcaaggttgg
    35221 cgcgtccggg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc
    35281 aggacgccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt
    35341 gcggggtgat ggaacagaca ttaccgctcc cctgctccgc gccgtggttt aaccgcacgg
    35401 gcagcagcaa acgggaagcg gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc
    35461 tggcgctatt acggcgcggc tggcaggtaa cgctttattg cgcggatgag gcccccgcac
    35521 tgggtgcttc cggcaatcgc cagggggcgc tgtatccgtt attaagcaaa cacgatgagg
    35581 cgctaaaccg ctttttctct aatgcgttta cttttgctcg tcggttttac gaccaattac
    35641 ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc
    35701 agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct gtagccgttg
    35761 aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc attacttatc
    35821 cgcaaggtgg ttggctgtgc ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac
    35881 agcagggttt gcagatttat tatcaatatc agttacagaa tttatcccgt aaggatgact
    35941 gttggttgtt gaattttgca ggagatcagc aagcaacaca cagcgtagtg gtactggcga
    36001 acgggcatca aatcagccga ttcagccaaa cgtcgactct cccggtgtat tcggttgccg
    36061 ggcaggtcag ccatattccg acaacgccgg aactggcaga gctgaagcag gtgctgtgct
    36121 atgacggtta tctcacgcca caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt
    36181 atcatcgcgg cagcgaagat acggcgtaca gtgaggacga tcagcagcag aatcgccagc
    36241 ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatgtc agtgataaag
    36301 aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta ggcaatgttc
    36361 ccgattatga ggcaacactc gtggaatatg cgtcgttggc ggagcagaaa gatgaggcgg
    36421 taagcgcgcc ggtttttgac gatctcttta tgtttgcggc tttaggttct cgcggtttgt
    36481 gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa ccgattccga
    36541 tggatgccag tacgctggcg gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga
    36601 agggtaaagc ggttaaggcg gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga
    36661 acgccttatc cgacctacgt gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc
    36721 aggcatggtg ctccagacgc cgcaacttac tgctgtgacg cctgctgaaa caggttttcc
    36781 cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccggcacc aatggctttt
    36841 tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc accactttcc
    36901 agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa caactcatca
    36961 tcactggcat ggtcaaccat accgtcgatt aatgccagaa tgcgtgattc aaactccgcg
    37021 atcatcttct ttcctcattg taaaacgtgg caccggcttc agttgagcgc ttccggccac
    37081 gggaactgtt ccgccgtaag ttccggcgtg tgataataat tttgtagtgc tttaatgaag
    37141 cgtgccgggc gttcaggaat gccgtttgct aggtaatcca tcacctgcgc atgtacccgc
    37201 cgttgaaaca ccacacgatc cggttcgaag tcgccttcca gattgtcgca actgacgtta
    37261 aacggatatc ccgccgccac acagaacaac cagtcgaacg cctgcggctt cacttcaaca
    37321 tcttcaaact ggctttgcgt ttgggcatcg cgtccgtccg ggcaatacca gtagccgaaa
    37381 tcaaccagct cacggcgcgc tttcccagca atacaccagt gcgaaatctc atgaattgcg
    37441 ctggcataaa agccatgagc aaagacgatt cggttatacg gtacttccgc atcagcagga
    37501 agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaattcatc ggcaaagcag
    37561 ctattaaaaa tttcaatcaa ctgctcatag tggtgtgtac tgttcattag ttcatcccca
    37621 accagtggag gatctcctgt ccgtggctat caaaaagtaa tttggcactc atcaccgctg
    37681 agacaataac aatcatcggg cggatcagct tttgtccttt gctcaacacc agtcgtgaac
    37741 ccatgcgcgc gccgaggaac tgccccacca gcatgacaaa gcccgtcgcc caaatcactt
    37801 tgccaccgag aataaacagc agtaaaccgc cgatgtttga cgttgcgttg agtaatttgg
    37861 cgtgcgccgt ggctttggcg aggttgaatc cgcacagcgt aacaaaggcc agtgcgtaaa
    37921 acgatccggc tgccggacca aagaatccgt cataaaaacc gacgcagcca ccggcaatta
    37981 acgcgaaggg taaaccgtac atccgacgct ggcgatcttc ttcacccagc tttggcatca
    38041 gcaagaaata gaggccgata caaattacca gaatgggcaa aatctgccgt aagacatcag
    38101 cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc gacaaaggtc atggcgatat
    38161 tgagtttctg atcgcttaaa ctaaccactt tgcggcgaat aaagtagata gtagcggaaa
    38221 tagagccgcc gcaggcttgc agtttattgg ttgccagcgc attagcggga gacatccccg
    38281 ctgccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag tcgataaatc
    38341 ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggggaaacc ataaacaggc
    38401 tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg aaggcggcaa
    38461 cggaggcggt gtcttcttct caggctttgt tgttcccggt tttggaggtt caaaccagct
    38521 ttgcagttct gccccgcaac catcgcctgg tggcggtaaa ggttgatctt cacactccag
    38581 actatcggca ggacaacgta atcgtacatg catatgcgca cgatgctgga accagggtcg
    38641 cactttgcgc aaccagtcgc gatcggtgcc cgcatctagg caaagttgtt gtttaatcgc
    38701 cggattaaca aaaatgcgcg tgacgttttt gtcctgggcg gcgagtttga tcaagctgaa
    38761 aatttctggc ttccacagcg aggagacaac gtgtttaccg tcgcgggaaa ctaagtccag
    38821 tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt gcagaaagat
    38881 atcgacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc caccagcagg
    38941 catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcacct ggctactcag
    39001 acgctggata aacatcacca gatccgggtg accgaaatag cgacgctgat cggtacgcat
    39061 gacctgataa tgttcggact gtatcggcag cgtgtcagcg ccgacaatac agccattaga
    39121 aaaactgcct atcgattgtg cgctacccgg cacaggttgg gttatttttt gccacggcgt
    39181 cgctgccagg ctagcgctac tggcaagcag agccagcagc gcaatcgcgg ttttgttcat
    39241 tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct gccgtaacag
    39301 gtgatccatt aaaacgatcg ccagcatcgc ttctgcgatc ggcactgcac ggatcccgac
    39361 acagggatcg tgacggcctt tggtgatcat ctcaacttct tcgccaaagc ggttaatagt
    39421 gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc atatgggcaa tgatttgctg
    39481 cccgctgctg ataccgccga gaatgccgcc cgcatggttg ctctggaaac cgtctttggt
    39541 gatttcgtcg cggttctggc tgccgcgcag cgccactacg tcaaaaccgt cgccaatttc
    39601 cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcggcat ccaggcggtc
    39661 aaagaccggc tcgccaagtc cggcggggac accactggca acaacggtga ctttcgcgcc
    39721 gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt cgattttgtc
    39781 cgggtccggg cagaagaacg gattttgttc aaccagcgac cagtctttga tttccagcgg
    39841 aatgtcgccc atctgggtca agcagccgcg gatttcaata ccaaatttct cggcgagata
    39901 ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg aagaacgtcc
    39961 accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcag catggcctgg
    40021 gcggaaaacg tctttaattg cgctgtaatc ctgagagcgc tggtcagtgt tttcgatcaa
    40081 caagccaatg ctggtgccag tagtaacgcc ttcaaaaaca ccggagagaa ttttgacctg
    40141 atccggctcg cggcgctggg tggtatagcg cgatgtccca gggcgacgac ggtcgaggtc
    40201 atgttgcagg tccgcttccg tcagcggaat gcctggcgga acaccatcga cgatgcagcc
    40261 gagcgccagc ccatgcgatt cgccgaaggt ggttacgcga aagagttgtc caattgtgtt
    40321 tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct ttataaatcg
    40381 cgaaatattc acgggccgcc agcagctgct ctttggtgag cataaacaca ccatcgccgc
    40441 cgttatcaaa ctccagccag gtgaacggaa catccggata ttgttccata agatgtacca
    40501 tgctgttgcc gacttcacaa atcagcacac catcatcagc aaggtaatct gccgcgttac
    40561 cgagaatgcg acgcgtcagt ttcaggccgt cggtgccaga tgccaggccc agttccggct
    40621 cgtgacggta ttcgtttggc aggtcggaca tatcttccgc gtcgacatac ggcgggttag
    40681 tgacaatcag atcgtattgc actttcggca aatcacggaa cagatcggaa cgaatcggaa
    40741 tgacgttgtg gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc agcgcgtcgg
    40801 gagagatatc taccgcgtcg acttccgctt ccgggaaggc ataagcacag gcaatggcga
    40861 tgcagccgct accggtacac atatctaaaa tatgctgcgg ttgcttgctg atgagtccgg
    40921 caaatttatt gttgatcagt tcaccaatcg gtgagcgcgg caccagcacg cgttcatcga
    40981 cgtaaaattc atgaccgcag aaccaggctt tgttggtcag gtaagccacc ggaatgcgct
    41041 cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt ttcgctggag gtcagacgcg
    41101 cggtgcgcat atcttccggg atatccagcg gcaggtagag cgatggtaac accagctgta
    41161 cggcttcatc ccacgggtta tcggtgccgt gaccgtacca gatatttgcc gcgctgaagc
    41221 ggcttaccga ccagcgcaac atgtcctgaa tggtttgcag ctcatttact gcttcatcaa
    41281 cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg cggctagttt
    41341 gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa atacgtttaa
    41401 aacgatcgat tgtgctacga gtcgggtaaa ctgtaggaaa attagaaata gagacagata
    41461 aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc agttgatggc
    41521 ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta aaaaaatcag
    41581 cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt atttctccga
    41641 tgagtttcag ccgttattaa ataccgaagg tccggtgaaa tatgttcgcc cggatgtcag
    41701 ccattttgag gcgaagaaac tgcgccgcgg cgattattca ccggaattgt ttttggattt
    41761 acacggtctg acgcagctgc aggccaagca ggaactgggc gcgttgattg ccgcctgtcg
    41821 ccgcgaacat gtgttttgtg cctgcgtgat gcatgggcac gggaagcata ttttgaagca
    41881 acaaacaccg ctgtggctgg cacaacatcc gcatgtgatg gcatttcacc aggcaccgaa
    41941 agagtacggc ggtgatgcgg ctttgttggt gttgattgaa gtggaagagt ggttgccgcc
    42001 ggagttgccc tgaggagttg agcgtgttgt gccaggagcg cggaatggca ggggcgaatg
    42061 accctgccag gtgagaaaag gtgcgctaat acatattcag tatgtaagtg aatcggcagg
    42121 tagaatctat gggctgtcga gatgtcgtgt ccacgcgtag cttcatatca tgcagttgcc
    42181 tggacgtgat cggttgcggc tctggcgtca ccgtcacttt ggcattatca cagccggaag
    42241 tcgagaaggt catctgctgt gcgacaaaac ggcagtaggc tccacgctcg tcaaaattac
    42301 tgccatcaca cagtttgccg ttttccatca ggaaagtggg cagtgtttgc tcaataacac
    42361 cacctgttga ctgcaacctg acgactttgt tatgtacaac ataattttca ttccaggaaa
    42421 catcatacgg tgccagggac tctgtcgggc agtcagagct aacttttgtt ggtcctataa
    42481 aaattccggc cccgaaacgc tgatccatag tactggcgct ttcaacataa gacgacacaa
    42541 tccagggaca accgtgtaaa tctccatcgt aataaacgcc aagtactaat tcattacatg
    42601 accaggccgt tactggagag agagcatcat actcttctaa tccggggcag gtagaatccg
    42661 gtaaggttgt attattgcgc tccatcctga gttcagggcc gggaggactc ccggtccaac
    42721 taactaaagg caaacttgct gccgcagttg aattagattc atacgtgcca tattcaactg
    42781 attgaggggt caggactgaa agatgaaaac gatactttgc aacaacgctg gtcgtccccg
    42841 cgggtacata gagtgtggcg atctttgtcg cagcctgtat cccagaagcg cacagagcca
    42901 gcaccaataa acatataaca tgacgtattt tatgcatttt atatatactc aaacgtagct
    42961 gtcaggctgg cggtgaaatc accgatggtt acatcacgac cgcttttcgc ctggatccag
    43021 gcccggaaat taatatcatt gttgccgttg gtaagcgcaa aagtcgcccc ggtggtgttg
    43081 ttaatatcca cccgcttgcc gtcgagcgtt tccatcccta tccccacgcc ctgcgcggtg
    43141 gaggtgtcgt cgactgccag aaaacccggc tgaccgctgt cttccgttcc ggtgagggtg
    43201 actttgacgt tgtatcccgc cggccctttg cagtctttca gcttaaacac aaccggcaga
    43261 tgcgcggact gcccggcgac catcaaatcc cggttgctga cggtgggaaa atgaatttcc
    43321 gccagattgg caccatccac caccagcgtg caggatttac tcagcaggtt gccgtaaaaa
    43381 tgcaggttgt tatcgactgc ccgtgccccc gtgctggcgg tcagcagcag gcctgctaac
    43441 agcgcataac cgggtgaata aatgtgtgct gttttcattg gtaatccacc cgtaaggttg
    43501 cgtaagcaga aaaattcgtg tccgtcagca ccgcatcagc cgctttgacc ggtaccgcct
    43561 ccagctttgg cttctggcta aaatcggtgg cgttaatgga aagcggctcg ttgagcttaa
    43621 aacgctgacc gtcatgctga agctcaattc cgaagcccgg tacatccgtc tcaatagcag
    43681 agtcgttgta ggaggtggca gtccccgtcc aggagagtgt catattccac tcgggatcgc
    43741 ggacatccga gtcgcagctc agttcgtagt ccactttctg acgaccataa ttgccatcaa
    43801 tgctgtcgat aataagatcg cgaaattgca cttcgatggt ttgcccgccg ctgatgctac
    43861 aggcgggcgg cgacatcagc gtaccgtgga atgtgatgtc atcttccgcc gccagcacag
    43921 ccccgcaacc agcgcacagt aaaaggagca ggaatttttt cattggtaat ccaccaccat
    43981 ggtcatcgtg gcgttaaact ccgacggcgt cagttgcacc ccgctctgtt ttaccggcac
    44041 cgctttcagc tgaggctgag tgttgacatt aaaaggcgtc cagttatttt caccaactgc
    44101 aaacagggag ttgtcggcgg cgttttctat ccgcaccccc aggccggcga tatccgtgct
    44161 gagaaccgtt tctccgttga tcacggcggt attgcctttg agctgcatcc gtaagtcatc
    44221 gctgacttta ttaccgcagt tgagggtgta tttcgcatcc tgttggtaat tggtaccatc
    44281 gatcctgttg attatcacat tgccaaattt cacagcgctt cccttcacgg agcaaggcgg
    44341 cggtccatcg accaggattc gcagcgtcag atccacctgt ttgatatcac tgccagcaac
    44401 agccaaaccg ctgatgcccc atccggccag caccagcaac cctgcgcccg ctacaaactt
    44461 gtgactcata agtccctccc ggcatcagcc tttgcggctc tgctcttcat ccacctggca
    44521 gctgttgccg gtgcatatga agtacagcgg catccgcgcg ccgtagtcgt tgatgtaagt
    44581 cagcaccggc acgctgtcca tcttcgcatt cagcggtgcg gaggttttcg gctccatcat
    44641 cagcggtgtg aatcccgccg gcgggttgcc gcctttttta gcgctggcgt tgctgatgat
    44701 cacgtagtac ggcgtcgggt tattgacggt gaagtcctgg ccgttacgga tgagcgtcac
    44761 cttatgctgc cacggacttt tcatgctggt gttctccagc gccttcggtc gccagaacag
    44821 tttgatacgc gtctgcagcg caatctgcag tgtgttcggt ttatttgatt tcggcggaat
    44881 ttcgcgcacg ttgaaataga acacgctctc gcggtcggcc ggcagtttgt tgatatccgg
    44941 cagcccctgc accttcacct ggccgttcat catcgcatcg atacgctgca ctggcggaag
    45001 caccgcaagc ggcgagctga ttttgttgcc ctttgcatcc tctatccagc tctgcgccag
    45061 ataaggcagt gtgggatcgt tgttgcgcag cgtgacgctg atggacttgt cgctttcgtt
    45121 aaacaccagc cgtgtcctgt cgggcgtcac cgaagcgtgg ctggagaaac tgaccactgt
    45181 cgccagcagc gccagtgccg ggacaacgat ttttgcagtg ttagtcagat taaacatgga
    45241 tcattcctta atcacgttac tggtttactg aaattgaagt cggaggttcc gttggcgtca
    45301 cccgctgtgt ctgttcctga atcgccggtt tgaccgccgg cgaagtgata ggtgctacat
    45361 tgcctttatg ctggcatggc agtaacaagc cgttaaacag atcggcaggc agcgggtccg
    45421 gcaggttgat atcgcaatgc gcaacaccac tccagaacac actcatgtgt tcgccaggtt
    45481 tcaccccagc cagataaaca ttgccgtcat catcgacaag gcccactgtc tgctcgttat
    45541 cattttttac ttctgcgcca aacggaggat ggctgccgtc gctcaggcgc agcaccgcca
    45601 tcgctttttg tccgctaatg acagcaaatt tacggtagcc aatggcacct tcagttagcg
    45661 tggcttgcac caccgactgg gttgcttcag cgttttcagg caatctgttg aggtcgatat
    45721 acgcctgatt gcggtaatag ttattgacgt cagaaacgac ggctttacca aacatattgg
    45781 tataaacagc agccccgtta ccttccaccg gaacatcggc aacgccatcg gcatcaatca
    45841 acaatcgtgt cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg gcagtaagcg
    45901 ttgcgccgcc ctgtaacgac aagcccgcag aggtgtattg cccttcatgg tagttcgcac
    45961 tgaggtcaac ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg ctggtgtgtt
    46021 tgtcactggt gccgacgttc aactgatagt gagtcgcgtc atcgacacgg ctgaaataac
    46081 cgacctgact gctgtccgtc ccactgccat agttaccgtt atagctaacg gtactgttgt
    46141 cgccccacgg catactgagc gaaatgtaca tgcctttgtc ggcctggttg tcatactcgt
    46201 aacggtagcc agtcatcgag atgctgacat tacgaatact gcccatattg aaatagtgcg
    46261 agagcatgat gttgtagttt gtctgctcct cgcgatccca gtaggtatgg cgggtatagt
    46321 tgagataaac cgaaacaccc gcatcgcgga agttctggtt ataagtggcg gtgtacatct
    46381 ctttgtcgtt gcccgtgcgt accattccgc tgtcactggc atccagatac tcgctcatgg
    46441 tcataaagtt ctcttccgag aagcgatatc cagcaaaagt aacgcggctg ttgagctggt
    46501 caaagtcttt ggaataactc acacggaagg agttaccgtc cagcgaacct ttgccataag
    46561 cggtgtcttt atccagtttg gtgtgcgagt gagtaacatc aaacgcgacc gcgccgaatg
    46621 tagacaaatc gcgaccgacg ccaagcgccg cagactgata gttttcatct cccagtgcgc
    46681 cgccatataa cgaccagccg ttagcgatcc cccaggaagc ttcagcacca gaaaaaaatt
    46741 caccctcgat atggtgtccc cactcttgcg gacggcccat catgatctta tagcgaacct
    46801 gacctgggcg agtgaggtat ggcatcgagg cggtgctgat gtcatattcc tgcacctggc
    46861 cgttctgttc ttcaatgcga atatgcaacg taccggagac agaatcacca agatcctgaa
    46921 tacgaaacgg tccagccgga acctgcgttt cgtaaatcac acgccccatc tggctgacgg
    46981 tcacttttgc tgtggtgtgt gccacgccgg aaatgtctgg cgcgtagccg cgcagattgg
    47041 gaggcaacat ttgatcgtca gtactgacac tgccaccaac atagttaaag ccatcaaaaa
    47101 tatcggatct gaggtaatcc tcgcccagcg ccagtttggc ttttaatgat ggtaacgccc
    47161 gccaggcata gtagcgactc cactcccatt ttttttgagt ttcatcgccg ctgaattcat
    47221 cgtcatcatc attactgcga gtatgttgat agttagtctg ccagtcagca cgcatacgcc
    47281 acggccccag gttaaccccg accgtcccgt tgccgctgat ctcgttacta tcatcaccgc
    47341 cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc ccggagatgc
    47401 cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg taagcctgtg
    47461 gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg ccttccagtt
    47521 gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc acgtcttctt
    47581 ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg ctcgcgtcat
    47641 cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta tttagttgaa
    47701 cctgtaaatt gtattttccg ggctcaacat atccctggct ggaaaaacgc ttcagatcaa
    47761 tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg tcttcagccc
    47821 agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga atccgtaatc
    47881 gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt tcggtcaaaa
    47941 tccatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg taagtaatct
    48001 ggaaggtggt atttgcttcg aaattaccca gatctggcgc atcagctgcg ccgaccagcc
    48061 aggctttaaa gttcagtgtc tgcttcgctt tgcctttatt ggtagaagtg tctttcacta
    48121 ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca ccgatcgcca
    48181 ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa taattgccgc
    48241 tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga gtatcaaata
    48301 cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta tttttcagtt
    48361 tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtccgga acgatagaac
    48421 acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc gcagcgattg
    48481 ttgccgtaga ggtgaaaacg cccatcacca ttgccgcagc aatagctgtt ttaacaaact
    48541 tactcattat ttgtccttaa actataaata attaaaaata taaacattac aatacattga
    48601 ttcagtcaat acccaatgtt tatacccagc cattgaatac ggcatgcgat ccaaaaagaa
    48661 caggtcgtag aaaattgtat tctttggtgt ggggccgcat agtgatgttt tcacccccct
    48721 gatgcaattt aattttacta a
//
LOCUS       DAZFKM010000019        47848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFKM010000019 DAZFKM010000000
VERSION     DAZFKM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..1142
                     /gene="phoE"
                     /locus_tag="ACHKAY_001217"
     CDS             87..1142
                     /gene="phoE"
                     /locus_tag="ACHKAY_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5699926.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1181..1582)
                     /gene="crl"
                     /locus_tag="ACHKAY_001218"
     CDS             complement(1181..1582)
                     /gene="crl"
                     /locus_tag="ACHKAY_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5699927.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1640..2884)
                     /gene="frsA"
                     /locus_tag="ACHKAY_001219"
     CDS             complement(1640..2884)
                     /gene="frsA"
                     /locus_tag="ACHKAY_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5699928.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2976..3434)
                     /gene="gpt"
                     /locus_tag="ACHKAY_001220"
     CDS             complement(2976..3434)
                     /gene="gpt"
                     /locus_tag="ACHKAY_001220"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5699929.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3695..5152
                     /gene="pepD"
                     /locus_tag="ACHKAY_001221"
     CDS             3695..5152
                     /gene="pepD"
                     /locus_tag="ACHKAY_001221"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5699930.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5209..5823)
                     /gene="prfH"
                     /locus_tag="ACHKAY_001222"
     CDS             complement(5209..5823)
                     /gene="prfH"
                     /locus_tag="ACHKAY_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5699931.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5820..6959)
                     /locus_tag="ACHKAY_001223"
     CDS             complement(5820..6959)
                     /locus_tag="ACHKAY_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5699932.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7205..7657)
                     /gene="yafP"
                     /locus_tag="ACHKAY_001224"
     CDS             complement(7205..7657)
                     /gene="yafP"
                     /locus_tag="ACHKAY_001224"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5699933.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7654..8709)
                     /gene="dinB"
                     /locus_tag="ACHKAY_001225"
     CDS             complement(7654..8709)
                     /gene="dinB"
                     /locus_tag="ACHKAY_001225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO5699934.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8780..9703)
                     /gene="lafU"
                     /locus_tag="ACHKAY_001226"
     CDS             complement(8780..9703)
                     /gene="lafU"
                     /locus_tag="ACHKAY_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO5699935.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9706..10569)
                     /gene="motA"
                     /locus_tag="ACHKAY_001227"
     CDS             complement(9706..10569)
                     /gene="motA"
                     /locus_tag="ACHKAY_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5699936.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10582..11298)
                     /locus_tag="ACHKAY_001228"
     CDS             complement(10582..11298)
                     /locus_tag="ACHKAY_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO5699937.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11318..11785)
                     /locus_tag="ACHKAY_001229"
     CDS             complement(11318..11785)
                     /locus_tag="ACHKAY_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO5699938.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11793..12854)
                     /locus_tag="ACHKAY_001230"
     CDS             complement(11793..12854)
                     /locus_tag="ACHKAY_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5699939.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12851..13162)
                     /locus_tag="ACHKAY_001231"
     CDS             complement(12851..13162)
                     /locus_tag="ACHKAY_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO5699940.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13167..13559)
                     /gene="fliS"
                     /locus_tag="ACHKAY_001232"
     CDS             complement(13167..13559)
                     /gene="fliS"
                     /locus_tag="ACHKAY_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5699941.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13582..14898)
                     /gene="fliD"
                     /locus_tag="ACHKAY_001233"
     CDS             complement(13582..14898)
                     /gene="fliD"
                     /locus_tag="ACHKAY_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5699942.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15105..16019)
                     /gene="lafA"
                     /locus_tag="ACHKAY_001234"
     CDS             complement(15105..16019)
                     /gene="lafA"
                     /locus_tag="ACHKAY_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO5699943.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            16505..17347
                     /locus_tag="ACHKAY_001235"
     CDS             16505..17347
                     /locus_tag="ACHKAY_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO5699944.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(17402..18379)
                     /locus_tag="ACHKAY_001236"
     CDS             complement(17402..18379)
                     /locus_tag="ACHKAY_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO5699945.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(18396..19325)
                     /gene="flgL"
                     /locus_tag="ACHKAY_001237"
     CDS             complement(18396..19325)
                     /gene="flgL"
                     /locus_tag="ACHKAY_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5699946.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(19340..20716)
                     /gene="flgK"
                     /locus_tag="ACHKAY_001238"
     CDS             complement(19340..20716)
                     /gene="flgK"
                     /locus_tag="ACHKAY_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5699947.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGISMEEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(20898..21197)
                     /locus_tag="ACHKAY_001239"
     CDS             complement(20898..21197)
                     /locus_tag="ACHKAY_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO5699948.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(21197..22297)
                     /locus_tag="ACHKAY_001240"
     CDS             complement(21197..22297)
                     /locus_tag="ACHKAY_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5699949.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(22312..22977)
                     /gene="flgH"
                     /locus_tag="ACHKAY_001241"
     CDS             complement(22312..22977)
                     /gene="flgH"
                     /locus_tag="ACHKAY_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5699950.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(23060..23845)
                     /gene="flgG"
                     /locus_tag="ACHKAY_001242"
     CDS             complement(23060..23845)
                     /gene="flgG"
                     /locus_tag="ACHKAY_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5699951.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(23924..24661)
                     /locus_tag="ACHKAY_001243"
     CDS             complement(23924..24661)
                     /locus_tag="ACHKAY_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO5699952.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(24661..25863)
                     /gene="flgE"
                     /locus_tag="ACHKAY_001244"
     CDS             complement(24661..25863)
                     /gene="flgE"
                     /locus_tag="ACHKAY_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5699953.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(25887..26600)
                     /locus_tag="ACHKAY_001245"
     CDS             complement(25887..26600)
                     /locus_tag="ACHKAY_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO5699954.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(26600..27031)
                     /gene="flgC"
                     /locus_tag="ACHKAY_001246"
     CDS             complement(26600..27031)
                     /gene="flgC"
                     /locus_tag="ACHKAY_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5699955.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(27034..27369)
                     /locus_tag="ACHKAY_001247"
     CDS             complement(27034..27369)
                     /locus_tag="ACHKAY_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO5699956.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            27451..28188
                     /gene="flgA"
                     /locus_tag="ACHKAY_001248"
     CDS             27451..28188
                     /gene="flgA"
                     /locus_tag="ACHKAY_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5699957.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            28269..28547
                     /gene="flgM"
                     /locus_tag="ACHKAY_001249"
     CDS             28269..28547
                     /gene="flgM"
                     /locus_tag="ACHKAY_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5699958.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            28560..28988
                     /gene="flgN"
                     /locus_tag="ACHKAY_001250"
     CDS             28560..28988
                     /gene="flgN"
                     /locus_tag="ACHKAY_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO5699959.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            29115..30044
                     /locus_tag="ACHKAY_001251"
     CDS             29115..30044
                     /locus_tag="ACHKAY_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO5699960.1"
                     /translation="MSFIECYEPEYVRKFLARPPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            30223..32667
                     /locus_tag="ACHKAY_001252"
     CDS             30223..32667
                     /locus_tag="ACHKAY_001252"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5699961.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(32785..33180)
                     /locus_tag="ACHKAY_001253"
     CDS             complement(32785..33180)
                     /locus_tag="ACHKAY_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO5699962.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(33183..33617)
                     /gene="fliJ"
                     /locus_tag="ACHKAY_001254"
     CDS             complement(33183..33617)
                     /gene="fliJ"
                     /locus_tag="ACHKAY_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5699963.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YFVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(33620..34957)
                     /gene="fliI"
                     /locus_tag="ACHKAY_001255"
     CDS             complement(33620..34957)
                     /gene="fliI"
                     /locus_tag="ACHKAY_001255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5699964.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(34950..35660)
                     /gene="fliH"
                     /locus_tag="ACHKAY_001256"
     CDS             complement(34950..35660)
                     /gene="fliH"
                     /locus_tag="ACHKAY_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5699965.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(35663..36673)
                     /locus_tag="ACHKAY_001257"
     CDS             complement(35663..36673)
                     /locus_tag="ACHKAY_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5699966.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(36651..38297)
                     /gene="fliF"
                     /locus_tag="ACHKAY_001258"
     CDS             complement(36651..38297)
                     /gene="fliF"
                     /locus_tag="ACHKAY_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5699967.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLERELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(38302..38643)
                     /locus_tag="ACHKAY_001259"
     CDS             complement(38302..38643)
                     /locus_tag="ACHKAY_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5699968.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(38658..39644)
                     /locus_tag="ACHKAY_001260"
     CDS             complement(38658..39644)
                     /locus_tag="ACHKAY_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO5699969.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            40031..40882
                     /locus_tag="ACHKAY_001261"
     CDS             40031..40882
                     /locus_tag="ACHKAY_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5699970.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            40875..41246
                     /locus_tag="ACHKAY_001262"
     CDS             40875..41246
                     /locus_tag="ACHKAY_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO5699971.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            41243..41995
                     /gene="fliP"
                     /locus_tag="ACHKAY_001263"
     CDS             41243..41995
                     /gene="fliP"
                     /locus_tag="ACHKAY_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5699972.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            41998..42270
                     /locus_tag="ACHKAY_001264"
     CDS             41998..42270
                     /locus_tag="ACHKAY_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO5699973.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            42272..43054
                     /gene="fliR"
                     /locus_tag="ACHKAY_001265"
     CDS             42272..43054
                     /gene="fliR"
                     /locus_tag="ACHKAY_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5699974.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLQALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            43044..44183
                     /gene="flhB"
                     /locus_tag="ACHKAY_001266"
     CDS             43044..44183
                     /gene="flhB"
                     /locus_tag="ACHKAY_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO5699975.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            44167..46260
                     /gene="flhA"
                     /locus_tag="ACHKAY_001267"
     CDS             44167..46260
                     /gene="flhA"
                     /locus_tag="ACHKAY_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5699976.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            46365..46643
                     /locus_tag="ACHKAY_001268"
     CDS             46365..46643
                     /locus_tag="ACHKAY_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO5699977.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            46636..46992
                     /locus_tag="ACHKAY_001269"
     CDS             46636..46992
                     /locus_tag="ACHKAY_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO5699978.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(47049..47753)
                     /gene="yafL"
                     /locus_tag="ACHKAY_001270"
     CDS             complement(47049..47753)
                     /gene="yafL"
                     /locus_tag="ACHKAY_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5699979.1"
                     /translation="MLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQ
                     ASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHN
                     ITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRR
                     ATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSQLAEPFWQ
                     DHFLGARRILTEETIL"
BASE COUNT        10442 a        13210 c        12699 g        11497 t
ORIGIN      
        1 ttaacaatct caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg
       61 cgcgttaatt aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg
      121 gcattgtggc atctgcatct gtacaggctg cagaaatata taataaagac ggtaataaac
      181 tggatgtcta tggcaaagtt aaagccatgc attatatgag tgataacgcc agtaaagatg
      241 gcgaccagag ttatatccgt tttggtttta aaggtgaaac acaaattaac gatcaactga
      301 ctggttatgg tcggtgggaa gcggagtttg ccggaaataa agcggagagt gataccgcac
      361 agcaaaaaac gcgtctcgct tttgccgggt tgaaatataa agatttgggt tctttcgatt
      421 atggtcgtaa cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat
      481 ttggtggcga ttcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg
      541 cgacgtatcg gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat
      601 atcaagggaa aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt
      661 cattgacata tgactttggc ggcagcgatt tcgccattag tggggcctat accaactcag
      721 atcgcaccaa cgagcagaac ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg
      781 ctacaggtct gaaatacgat gccaataatt tttatctggc aactttttat tctgaaacac
      841 gcaaaatgac gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg
      901 ctcaatacca gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga
      961 aagatattga aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggggctacat
     1021 attatttcaa caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg
     1081 ataacaaatt gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt
     1141 aatgattatt gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg
     1201 gctcgtcacg aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac
     1261 gcagcttctc gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt
     1321 ttaccggaac actcttccag tcgcctgctt tatcgaacag accaaactgg taactgtagg
     1381 taaaacggga ttcctgcgct tcaagctcca tccaccagcc ccagaactca cgcacttccg
     1441 gtgccggttt cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc
     1501 acttaccttc acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg
     1561 ggtgtccact cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt
     1621 ggcaaaattt agcaaatttt taacacaagc gtttttcgat ccagccggtg atttcctgaa
     1681 gacctttgtc aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag
     1741 cagatgatga ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt
     1801 agcctgataa cattggcgtt gggcagcgac gccccagcaa tccttgcact tttaatgaat
     1861 agcgattcag ctccacgcgt aacgcgtcat cggaagcatc atgcatcccc aaacgactcg
     1921 ccagaacgtc aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg
     1981 tatgaactac cggaccaaga caggcaaccg ctttcagacg cggcgattca agatatgcca
     2041 gacgcacggc aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca
     2101 cccacggtac gttaggcagc gcctttaaga cgtgctgatg caacaggctg gagtcctggg
     2161 tgagcttcca ttttgaagaa aagcccaccg atggcatatc aatagtcagc atcgcaatgc
     2221 cgcgcggcgc aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac
     2281 caccacacat taatactgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg
     2341 tgatgggcgc accgccgggt acggtaaact ccatctgccg catcgtgccc ggcagacgct
     2401 gggcggcctc ttcataggcg cggtttgata aagcctgcgc ttgctcggcc aggtcatctc
     2461 ctttcagatg aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac
     2521 cactgagttg cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg
     2581 cccactcata aatccagttg ccaccacggt agccaatcac agtatcgtat aaatcatcgt
     2641 cggtacgttc agcatcgctc atcacaatac gtgcctggac gtcgaggatt tcacgtgggt
     2701 caataccgcg ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt
     2761 taccatcaag agccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg
     2821 tcgaggtttc aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg
     2881 tcatggatgc agcctccaga atacttactg gaaactattg taacctgcct gaagttaaaa
     2941 agaacaacgc ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg
     3001 aatacgacgc ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca
     3061 tagtcatcaa ccagcggacg accagccggt tttgcgaaga tggtgacaaa gtgcgctttt
     3121 ggatacattt cacgaatcgc aacagcagta ccaccggtat ccaccaggtc atcaataacg
     3181 atgaagcctt cgccatcgcc ttctgcgcgt ttcagcactt taagctcgcg ctggttgtcg
     3241 tgatcgtagc tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt
     3301 aacgcacccg gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa
     3361 ggcatcagtc ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg
     3421 tatttttcgc tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag
     3481 gggaaaatag gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat
     3541 gagtccgcgt ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa
     3601 catatctcgc aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat
     3661 actgttttgc gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc
     3721 acagccgctg tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga
     3781 agagcaactc gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg
     3841 cgatcaggta ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa
     3901 accggtcgtc ttacaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca
     3961 tgacttcacg aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg
     4021 caccacgctg ggtgcagata acggcattgg tatggcgtct gcgctggcgg ttctggctga
     4081 cgaaaacgtg gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat
     4141 ggacggtgcg ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga
     4201 ctccgaagaa gaaggtgaaa tctacatggg ttgtgcgggg ggtatcgact tcacctccaa
     4261 cctgcattta gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa
     4321 aggtctgaaa ggcggtcact ccggcggtga aatccacgtt ggcctgggta atgccaacaa
     4381 actactggtg cgcttcctgg cgggtcatgc ggaagaactg gatctgcgcc ttatcgattt
     4441 caacggcggc acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc
     4501 agctgataaa gtcgacgtcc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa
     4561 cgagctggca gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa
     4621 agctgccctg attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa
     4681 cggtgtgatc cgcaattccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg
     4741 tgtggtgacc atgactgaca ataacgtaga aattcactgc ctgatccgtt cactgatcga
     4801 cagcggtaaa gactacgtgg tgagcatgct ggattcgctg ggtaaactag ctggcgcgaa
     4861 aaccgaagcg aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca
     4921 tctggtacgt gaaacctatc agcgtctgtt caacaaaaca ccgaacatcc agattatcca
     4981 cgcgggcctg gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat
     5041 cgggccaact atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg
     5101 tcattactgg acgctgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga
     5161 tttgctgccg gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat
     5221 aaaagccatc cctgtaaatg tccgtctcgg gttgccacgt tcaatctggt gatggaacat
     5281 tcgccgcagc gatttcagca ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc
     5341 aatcagcaat cgtgccagcc gcttgttagc atgctgacta cgctctgact gaaccttcac
     5401 gctaataccg gatgccaaat gcgtggcgcg taccgccgag tcggttttat tgacatgttg
     5461 accgcccggc cccgacgaac gcaacgtctc ataacggatt gcatccgatt gttcctgctc
     5521 atcagcggta aaacgcccaa tgcccagaaa ccagtttttg cgcccatgat gaggccgata
     5581 cggactcgga caaatccact gaatagtgcc acaccagctt tcgcttaatg cccatgcgtt
     5641 atcgccatcc agagaaatca gcgccgaacg cagcgtgtca gagtagtggc ccgtttctgt
     5701 ttccagcacc gttaccgcga cgtcttgtcg ggcagcttct ttaattagcc tgtccagcgc
     5761 ctttttcact gcgagacaac attcttccgg cccctgcgct gaggagagtt gtagcaagat
     5821 catccttttt tccctccact gtttttgagc gttagcaccg gacgcagccg cgcgacagga
     5881 ataattaaac cggcctgcac cagacattgc accacgcttt cagccgattt ataggcttgt
     5941 ggcgcttctt caaagatgag ttgtttatcg cgacaaatta cccgactgcc aagttcagta
     6001 cgcgagagtt gcgtcgccgt gtatttcgct gccagacgcc ctttacactc ggtgcgcccc
     6061 catttacgcc ctgccccatg cgccagcgag tggagcgttt tttcgttcgc aacaggctgt
     6121 acaatccagg agtaatcacc acgtgaaccg ggaataatca ccagaccgtt gtcatccggc
     6181 gtggcacctt tacgatgcaa ccagccctgt tgatcgccga tttgacacgc gctaacaaag
     6241 ttatgcgcca catccagaac cggacttccc gtggccttaa cttgttgcat tatgcgcaaa
     6301 gcgatcatct ggcgattaat acacgcaaac gccagcgcat cgtcatgttc cgcaatatac
     6361 cgtagcgcgt cgtcactgcc ttgaggcaaa ccatgatgcg agaacgaggc aatatgccgc
     6421 tgtaaaatag actgccccac cccccgcgag ccgctatgaa ccaacagttg cagatgttgt
     6481 gcatccagac ctgccagtgc aaacagttca gcgttgatga tttgatcaac ttgttgcagt
     6541 tcagcgaaat gattgccgcc accgatagag ccaagcgagc tgcgccacgg atgctgtgca
     6601 aatgctgacg gcaggttttc ctccagccag ctttcttcag caacgtcatc cagatctgat
     6661 aatcgctttt caaacttatc ggcgttgtat ttgcgagcga gaatatctgt ttgccatagc
     6721 gccataccgc agccgatatc attgccgacc agtgccggat aaaaacgacc aacagagaag
     6781 aacgctgcgc caatcggata gccgcgtccg ggatgtaaat ctggcatccc cactacgcgc
     6841 tgcatgttgg gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac
     6901 aggtcatcag atgcgatggt aaataccgca tcggataagg gacgaatata attgcccata
     6961 taccattccg taaatagact cgtcatactt caagttgcat gtgttgcgac ttgaattatt
     7021 gagagtaaca attaaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat
     7081 taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt
     7141 gcagtttgtg gaataagaaa acgcgcacat actaggcttg cgttattttc attgcaagct
     7201 ggatttaatg ttgctgttta taacgcatat aaaaattaat aaaccactct ccccgacatt
     7261 caacgcgttg ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta
     7321 tgcttgcgtc caccgtaagt tcggattcag acttaatcaa aggttttaac aaagcgctgg
     7381 caaccccacg gcgggtgtat tcagggtcaa caaataacat atcgatatag tgttcaacgc
     7441 aggtaataaa gccgaccagt tttgcattaa tgactgcaac ccgcacttgt gatttcgcga
     7501 gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat
     7561 aatgctgact ggcagtcatc gtaaccgctc taatgaaaat agcgcatagt tgctgaaaat
     7621 cgccaggctg atagtctctt atttgtatgt tattcataat cccagcacca gttgtctttc
     7681 catttgcggg tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg
     7741 gcgttcatcc caggttttac gcgcggtggc gattaagtca gctttattca gccgcggcca
     7801 gacgtgctcc tgagtggttt gctgaaaatc atcaaacttt aatttcactc cctgacgggc
     7861 gatcagtaaa tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc
     7921 gataatcgct tcacattcag accagtgatg aatatcttcc gccatcgtgc gttccacgcc
     7981 gacggatttt cgtaaccgtt cgctgttaac gtcgcgctcg tcaatcccct gactacgctc
     8041 ccacaaaatg cggccaaatt tgccaaagcg tttaagcagc atcaccagat cacacttttg
     8101 tacatcaccg caggtccgca gccccatcgc ttccagtttt gccgccgaga ctttgccgac
     8161 gccggggatt tttgccagcg gtaaggtttg taaaaatgcc ggaacttctg ccggcgtaat
     8221 cacaaactga ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa actttactgg
     8281 tgccacgccc gcagacgctg tcagttgcag ctcgctgaaa atcgtctggc ggatttcctg
     8341 ggcgatgagg gtcgcagaac cgtggcaatg gacgctatcg gtgacgtcga gataagcctc
     8401 atccagtgac aacggttcaa tacgcgaggt gtagcgcgag aagatttcac ggatatgatt
     8461 tgaggcttct ttgtaggcgt caaagcgccc cggaagcaag gtgagatgcg ggcataattt
     8521 gagcgccatc cctgtcggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc
     8581 ggtgctgatc accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag
     8641 ggcgggattg tcgcgcatct ccaccgctgc gaaaaagcag tccatatcca catgaatgat
     8701 tttacgcatt actcacctct caacactggt aaagtataca gtgatttcag ggtttgagaa
     8761 atgcgtaaag attcagcatt taacgcgttc gctgcgaaag cacctgctgc ttatccagct
     8821 tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat
     8881 cggacgcact tttggtcagc accataatct caatgcgccg gttgcccgcg ctttgcggat
     8941 ttttggaatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg
     9001 gcattccggc ctcttccagc acccgacgag ccgaaagcgc gcggtcaccc gaaaggttcc
     9061 agttgttgta gatattgttt ttgtaagcca tcgcatcggt atgcccggta ataataattt
     9121 tgttatcgag cgagtcgaaa actggcgcga gctccaccag cagcgtttta aagaacggca
     9181 taatctgcgc gctgccgcgt tcgaacatat tgcgattctg gtcgtcttta atcagtacgc
     9241 gtaatccctg gggaacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa
     9301 tggtgttgat actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg
     9361 ccgttttctc attcacatca cgcgcttttt tctccgtctc ttccggcacc gccacggtgg
     9421 caggttttgg cggatgagac gggctgattt tatttaacgg cgacagcccg ccaccgttaa
     9481 aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt
     9541 tgacaatcca cagcgtcata aacagcgcca tcatcgccag ggtaaagtcg gcgaacgcca
     9601 ccttccacgc cccgccatga tgcccggcat gattcttttt gatctgccgc cggacgatgg
     9661 tggtttttgc cccacgctcg cggcggttgc ccgtctttct catgcttatt cttgctccag
     9721 catcgcattc acccacgcgt cgaggttggc gaaggtcggt ttggacgcca gatgcagcag
     9781 cttacgcccg gcatccaccg ccagcagcgt cggtttgccg cctgcctgcg ccaccagcac
     9841 agtgcgcacg cattccagca aagaatgttc tgcccgcgcc tgttgttcca tggcgttggc
     9901 gagcgggtcc atcaggcagt agcagataaa gacgcctaaa aacgtcccca ccagcgctgc
     9961 cgccactttc aggccgatca gggcaataga gccgtcaatc gactgcatgg tgatgatgat
    10021 ccccagcact gctgcacaaa taccaaatcc cggcatcgct tcggcggtac gttgcagcga
    10081 gcgcgacggc gtgagcagcg actcttccgc agtgtccagt tcctgatcga gaatgccttc
    10141 cagttcatgg gcgtcgattt tacccatcgc catcaggcga aagttatcgg caataaaggt
    10201 caccagccgc ttctgggtta acaccagcgg gtatttctgg aaaatggtgc tctcttccgg
    10261 ttgctcaata tgttgatcca gcatgcgcag accaccgttt tgcaccattt cgagcaactc
    10321 atacaggcac attaatagct gacgctgaaa ctccggcccg agctgttttt tactgatcac
    10381 acctttaatt tgatgggcaa tctctttcag cacatgtttc gggttaccaa taatcatcgc
    10441 cccgaaaccg gcacccaaaa taatgatcat ctccgccggt tgccagatag ccactaattg
    10501 cccgcccgct tcaaaataac cgccaaatac gcagccgagc accaccagta aacctaatat
    10561 tttttgcatg atttcttcac tttaattcgg atagacaaaa tcacgaattt tctgcgcgat
    10621 ctgtttattc agttgacaaa ttcgggcctc ggttagccct aataccagcg caatctcttt
    10681 gagattcatc tcatgctggt aatacatgga gaggatcagt tgctcttttt cgcttaattg
    10741 tgaaagcgcc tgttgcagca tttgctgagt gacaaactga tcttccagct cccggccaac
    10801 aagcggaatg cctggccctt cgccgttcag cagttcgtcc aggctggcga gcgtttccgc
    10861 cccttccagt tgctgatatt cgagataatc ttcatgtgag atcccttcca cggccagttc
    10921 tgaccactcc ggttcgtgcc ccagtttttt gcgcgtttcg cgaatcagat ctttcatctg
    10981 atgatatttc tgtcgcagct ggcgcggtcg ccagtcgaga gcgcgcagct catccaggat
    11041 cgcgccgcga atacggtgca cggcatagcc cgcaaaacct tcgtccggca gcccgtagcg
    11101 gcggatggca ttaagcagcc ccattaacgc cgtttgttcc atatcctgac gatcaataat
    11161 gcaattacac tgcggggcaa gttgccgcac cactttacgc accaacggta aataggcctg
    11221 caagtagtgg ctctcctgct gcggggttaa taccggcgcg gcagcaaatt cttctgactc
    11281 gaatacatcc tgtagcatgt ttatatccgc ctggtgatta ctggaagacc acttttttca
    11341 ccagcaaatc cttatacggt gcgctcattt tgcgacgctg aagatctttc tttaacgcgt
    11401 cagaaatgag tttgcggatg gcagatattt tcatgccgcg caaatcttca aatttcattt
    11461 ccgtcaggca ttcaaccgca atactttgat acagcggctc ctgatgtttt attttttccg
    11521 cttctttgtc actggcgacc accatcacta attccgccga caaataatgg tccgcgccgt
    11581 tgttgtcatg cagcgtgaca atggtttccg gcaacgtcac aaacacgctt ttgctttcat
    11641 ccagggtttc caccgtttcc gtctgcgcgg cggcagtcgg ttttcctttt ccggcataat
    11701 gatgccagac accccatcca gctccggccc ccaccaccaa cgccagaacg gcgctgacca
    11761 cgcccgccac cacgattttc ttcatattgc ctttacactt tgatcaaaac ggattcgttc
    11821 tgttgaaatt gaggcgcatc atcaagctgt aaggcggcac tcacctcttc ctgctgatgg
    11881 cccgactgtt gttgttgctg cgactgccct tcggaggaaa cctgtacgtt cacctccata
    11941 aaattttgcg ccgtcagatg ctggcggaga tcgtcgctaa actgctgtaa cgcgcggcaa
    12001 acttcacttt ggttcgcgcc gatgtgcacc atcagtttgc cgttgtcgag ttgtacggcg
    12061 atctcgagct tgccaagcga cggtggatcg agacggatgg tggagatctg ctgttgtttg
    12121 ttgagctgaa agtggatctg atctttcaaa agcgtcgtca gtttttcgcc cagttcgtcc
    12181 atcgaaagcg tggctggttt ggcgacaatc tcctgatgat cgctgagcag ctccggcgtc
    12241 ttaacctgca acaccgcacg cgcaataacc ggttcactgt ccggcacttt ttccgtttta
    12301 cgctcgacct gcgggcgcgc ggtcacacgg gttagttcag atttcgggcg agctggctgt
    12361 gttgagacgc gcggctgcgt cggcgtaatg gcatgtaaat cttcactagc gtaagtcgcc
    12421 agtctggcct gctgttcggg cttcgcctgc ggcaactgac tgatcagttc ctgcaactgc
    12481 ggcggcaacg gcgtgagttc ctgctgttgc gtctggcgtt gcggcgcgtt ttgcaccacc
    12541 gctttcgtca attgctggat aaccggcgat gttgcgacgt ttcgcggctg cggcgtgtcc
    12601 tgatgcggcg cggcaggttg cggaagtaac agcgccatta gcgcctgcat cgccaccgga
    12661 tcgttctcct gcgggcgcgt cgcctgttgc tgactggttt ttgcgctatg aacgcgctcc
    12721 gccaccgccg cgaccgccat tttcggcagg gtaaacgcgg gcgcgttttc accgctcacc
    12781 ggcggtaaaa tatccgcctt caacgaagcg gtttccgcaa gcgttccgag cgtggcaagc
    12841 aatgtcggat tcatgacttc tccgaaagta aattaacaat tgagtaggcc agtacgcctt
    12901 cctgatgccg ctgatgctgt tccatacgcg ctttcaacac ttctgcgcgc tgctcgcgcc
    12961 gtttaatcag ggcgttaaac gcgtcataca gtcgcgcttt cactgcatcc aactgcgccg
    13021 catcggtttc cgccgccgcg taatcgtgaa actggcgtat caactgctga tgcagtgccg
    13081 gaaggcgtcg ccagcgttgc ttgtcgaggg cttcattcat cgcctcgacc agtgcgaaaa
    13141 gtttcctgcg ttgtttttcc atcgtcttaa cccagcttgc ccgaaagccc ttcccagcct
    13201 tcgcgcaggt tgcttaagat cagcatcact tcatcgattt tctctgccga cagttcgccg
    13261 ctggcctcat acaatcggta aacgcagtgg tcatagagtc gcgaaaggtt gaccaccagc
    13321 tcgccgccag tttcaaactc cagcgaactg gtgagcgcat tgagaatgtc gatgcattta
    13381 ttgatgctct gggctttctt ctcgaaacgc cgcccttcaa tatggctttt ggcgcgttcc
    13441 agttcgtcca tcaggccaga aaacagcacc agcaccagtt ccagaggcga tgccgccgca
    13501 gtacgcgccg cgagatcgat ctctttatat tgcgaatagc cgtcctgtac ttggtacatc
    13561 tttagggata ttccttctga gttacgcgaa tgccgccatg ctggctttca tggtgtacac
    13621 ctcgaccagc gtgttggtgt actcctcgag ataacggtcg tagttggcgt tataggtatc
    13681 agtaagctga tcgccttcgt cctgaatttt gctcatctga tcgtcaatgt tctgctggcg
    13741 cagggtgatg atgccgttgc tggagtccgt gtaggtgtta ataaggtcgt ccatctgcgc
    13801 caccatgctg ttgtcgccaa cgaaaataga ggtcagaccg tcaggatttt tcgccatttc
    13861 gtcgttgaac tggtcggagt caatctgtaa gtggccgtga gaatcgaggg tgatgccata
    13921 gtcaacaatc gacacgccgt tgtagctggc atgggcgatg tcatcgagct ggttcgccag
    13981 tgaactgagt ccggcgtcgc ctgcaaatac cccggcgctg gtactgtcat caccgtgggt
    14041 ggttagcgaa tcaaccgtat caatcaaggt gttataggca tcgacaaagg tctgtacttt
    14101 ctcctggctg gcgctggaat cttcgctgat gttaaaggta gtgagatcgc tgtcagaatc
    14161 gctgacttcg gtgaaagtca tggtgacacc gggaatcaca tcatcaaacg tattgctgct
    14221 gttggtgatc tcaggccccg tggcgctgcc aagatgaata atcgcgtcct gagcggagga
    14281 aacatccgtg gcaacgggcg cgctggtgct atcgttgctg gcatcatgcc ccgttacgct
    14341 caccgaaaac gcgctctgcg ccccggtgct atccgaggtg agcataatcg ttgtggtgcc
    14401 gtcggttttt accagcgccg ccgacacgcc cggattatca tcggagtcgt taatcgcatt
    14461 caccagttca ctggcgtcga taaaaccgtc gccgtcacca ttctgatctg ccgccgacag
    14521 atcgatatcc atggtgctgt cgcccatcgt cagttcaaaa gtgccggtgg cggaaaaggc
    14581 gtcgtcgccc atactgaacg tggtttgctg cccctgtgcc agttgctcaa cgaaaaatga
    14641 gtagcttccc gcctgcgcct gggaattggc ggaaacgctc gccgagtcat tattgctggt
    14701 ggcggcaaac gtcaccgggc cgtcggtatc gctgttcagc gcgtcaacgg cgctctgaaa
    14761 atcgctcaac gctgaactga gcgaatccag gccgctgctt tcagcatcca gctctgtttg
    14821 cttctcctgc aagtttgccg cctgagtggc gacatccgca tacgcgattt cttgcgcaat
    14881 ggttcttggg ttgatcattg atgactccgg ataacgatgt caggttattg gcaaaaggcg
    14941 tgccaatatt ttttatgtga ataatcaata gattaaaaaa caggcggaaa tggcgtttcc
    15001 ggtctggcgg aaaaaagcct gccgcagaaa gcacaacacc gcccgcaggc ggtgttgttc
    15061 gtagtccgaa taaggcgttt acgccgcatc cggcaagtcc gccattactg cagcaggctg
    15121 gaaaccatgc tggacatgct gttggactgt ttcagcatgg tgatgccagt ctgcatcagc
    15181 acctgctgtt tggtcatatt ggacgcttcc gtcgcgtagt cggtatccat aatgttaccg
    15241 atagcgactt cggtgttgtc ctgcatactg gtcaggttgt tggcggtgtc gttcagacgg
    15301 ttgatagacg cacccagctt ggactggatc tgcgaaacgt catccatcgc ggtagagatg
    15361 ctggtgatca tctggttagc cgagccagac gcggtcagct cagtaccgcc agaaacacct
    15421 gcagtacccg tggtgtctgc ctgatccgcg ctaaaggagt tgctgatagc agaaagatcg
    15481 gtcaccagcg tgttcagttg gctggagatg ttcacggtca tggtgtcgga agattccgcg
    15541 cccacctgga aagttaccgc gctctggaac agaccgtcag taccggtgtt gctggtaccg
    15601 gaaacaccga acaggttggt gccgccgtaa gtggtgtttt gcagcatgtc agacatctgc
    15661 tgacccagtt cgtcgtattc gtcctgcatc gcctgcagat cgtcgtcgct gtaggtgccg
    15721 ttcgctgcct gagttgacag gtctttcata cgacccagca catcagacat ttcatcgaac
    15781 atactatcgg cggtttgcag catcgcggtt gcgtcattga tgttgctcag cgccacgccc
    15841 ataccgctgg actgtgctgt cagacggtta gcaatttgtt tgcctgcggc gtcatcggca
    15901 gaagagttaa tccggttacc tgtcgccagg cgttccatag atgtagacaa agaagagctg
    15961 cttttgctga ttgcattaac ggcggccatt gaggcgttat tggtgttaat agataacatg
    16021 atactgctcc ttgggattaa tcgttttcgt ttcgataccc tgtaaaaacg acacctaagg
    16081 agcaaactta aaaccgccgt taaaaaaatt tcactgcgat ttatctgatg ccacttttag
    16141 ctaaaaaaag cagagtttaa attcagataa aaagattatt agcaatatct gccacatggc
    16201 caggtatcaa aatatgtctt tatcaaacgc ttgatatagt taagattacc atcaattaac
    16261 gcgttttctt ctcaatgtac tgatgccaaa atttacattg cgttgcaaat agtacacatt
    16321 tggctattgt accagtcctg tcatttcgtt acatttccat gccatatagt gattaagaat
    16381 aatcctatta ccataaaata ttagttatca cgcagaaaaa tcatcttctc agggacgtat
    16441 aagactgtgc tttatgcaaa gcactatcag gaaacattta tcccttgcta aggtaaactc
    16501 aactatgagt attattatca acaactggcg gatggacccg tcgcttaatg ccttaataca
    16561 ttgcgaaacg ggtgaaacac gtcgtctcgg cgaataccat tttattttgc tggaaaaatt
    16621 agcaaaaaat gccgatgtgg ttttatcgcg atcttattta tgtgccgaag tatggaagaa
    16681 ccgcattgtc ggcggaaaca gcctgccgac ggcgatccat gcgttgcgcg ttgctatcga
    16741 tgatgatggc aaacaacaga acattattaa aacaattccg aagaaaggtt acttatgcaa
    16801 taaagagtat gtgtctttgc cggactcatc gcctgcaaaa aaactgatta ttaccgacca
    16861 gatacaggaa acagtaccag aagagatatc ttctacaaca ccgcctgtgc ctgttagaaa
    16921 aaaacataaa gtaataatgg ggctagcgtt agccgcggct gttatattta ttggctcgac
    16981 cgtggggtat tcacacttga aaagtacacc tgatgccccg caactggtaa aagagtcaat
    17041 taacagtcca agaataaaaa tatttcacct tagttctgga aaagaaaata attccgtacc
    17101 gttactgtca caaaccctcg caccaggaaa agacaaactc gataatttat tgtcagcgca
    17161 taatatgaca atgacaacct attataaata tgttcgtaac cgactggaaa gtgatattgt
    17221 tctgcgcaac cagtgcaatg gtagctggca attaactttt aatgtggaag gctggcagaa
    17281 cagcgatatt aatagcgcga tgtatcagaa tttagagaag ttgttaaata ctttgcagaa
    17341 atgctgatga gtgccaatat atgccggatg cgacgctaaa cgcgtcttat ccggctgata
    17401 attaccccaa taaattcctc accgtctggc tggaaattcg cgcctgacca ttcaccgcct
    17461 gcaataaaac ctgatagtta tgattcgcgc tatccagcac gccgccgagg ttttccgacg
    17521 cctgcaccgc gttttccgtt tgcgggaagc gcgacatgcc gtcgatcaac tgccgcgcct
    17581 tttcctgctg taccgccatc tgcgcacgct gctgattaat gctttctaac gtctgagaaa
    17641 ttcccgcacc gccctgttgc aggctttgag ctaaacggtc tgcctcagac ggctcggcaa
    17701 aagttttcag cggcataaac gccgacttat cgccatcggt gcgcacgctt aaggtacgtt
    17761 cgatatgggg ccactgtttt tcggtggtgg agaaactgat gccgtccgct tgctgatgta
    17821 gctgcacgcc cacgcggcgc agggcgttgg tcagccgcgt ctgatactgc gcggcgctgt
    17881 catcctcaga cagcatcacc gccgaaagct gagtctgccg cccgtcagag acgctgaaca
    17941 tgcgcgttcc cggcgtggga ttgtgtacca gattcgccag atccggcgag tgaaaggtca
    18001 cccgcgcttc gccctgtaac accggctgca actggcggtc caccgcaccg ccggaaagcg
    18061 cggtgcgttt gtccagcatc tgcatcagcg ccgtattctg cgcctgcccg cctttacgct
    18121 ggctgtgacg atagtcgagc aactgctgct ccagttgccc gagataatga tccgcctgct
    18181 gcaacgtggt gagctggtcg ttaagctgca cgctgtaacg ctgcgggcga gtggtaatca
    18241 gcggcgaggc cggatattca ctcgccgtcg ccgggagttt ttgccgcacc gcctgagtgg
    18301 gcgcaacggt tgacgacgat accgcagcgc tatgcgcgcc gccggtggcg agtgatgaag
    18361 atgtaagtcc gacctgcata ctgttcctga aagagttaga gaacgctgaa caaattcaac
    18421 tcactcactt tcgagtaagt cttgagggta acttccattg ccacttccag tccggtaaag
    18481 gtgatcgacg ccgggccgta atcgagatcc tgtaatgaac caatcagctc atcattagag
    18541 gtggagatat cggtctgcgc atcgctcagc atcgacatgg tattttgcgt ctcgccgagt
    18601 gacgcaatcg aggcgttgag gtcgtccgag gcggtgtcca ccacatcaac ggcattctga
    18661 atatcgctct gcacctgcgg atctgccggg tcgacgtccg ggttttgcaa ctcctgggat
    18721 agggaattca gggtgttgag cacgtctaaa ttgctgccaa agaaatcact cgccgccacg
    18781 ttggtgtcca cttccacgcc gttcgacacc gtggtctggc ggtggtcgct gttaccctga
    18841 tagctgtagc tgtcggtgac gccatcgccg tcatcgtcca ctgccacaat cggcggctga
    18901 tcgttgatgg tgccgccaaa cacgtagtgc ccgtcttcgt cctgataatt gagcgccgcc
    18961 accattgatt cggtgagcga ctcaatatcc tgcccgaggc ttgcgagtga atcggcggta
    19021 ttggtgccat tcgccgcttc cagcaggtcg tcgcgcaccg ccagcaggct gttgttgacg
    19081 ccatcaagga tcgactcctg ctggcttaag cccgccgacg cggagtcgat attgctctga
    19141 tactgctcaa tcgccgactg ctggcggttt aactgggtga ttcgcgtggc ggcgatcggg
    19201 tcatccgacg gctgcaaaat ctgcttgccg gttgccatct gctcgacaac atgcgccaga
    19261 tcgccagaga gattattaaa actttgcgtc atcgagacgt aggtttgttg ggtagttact
    19321 cgcattttcg cgctcccgct tagctgcaca tttccagcac cgaatcgaaa atctcggccc
    19381 cggcggaaat gactttcaga ttggcttcat aaatttgttg ataggtgatc aggttcaccg
    19441 cctcttcctc catgctgatg ccgctgacgc tgctctgctg gttttgcgct gcggaataga
    19501 cattggacgc ggcatcgact tccgtctggt tttgctgact gtaaatgccg atattgctga
    19561 tgattgacga gcacgcctgc ccgaccgtca cgctgccgag gttggctatt tccagcggtt
    19621 cggtggagat attgatcagc gcctgcaggt tgtcgctgtt gccgctttcg tccggtgaac
    19681 tggaaaacgc cagctcatcg gcggtgatat tcgggttaac ggtcagcggg ccatcggcat
    19741 tgctggcgtc ataaataaac agcggctcgc cggggttacc gttgagatcg tagccctgcg
    19801 ccagctggtt gttgaccgca tcggcgaact gcgacgccat gctgttgatg gtgtcggtca
    19861 gcggcgtcag cacatcgttt tgataatcaa acagtgcgcc taatgaaccg ccggtgtcgg
    19921 tggtcatcgt cgaggtggtg ccagcgaagg tcagcgacat ggtcgacgtg ccatcggcgt
    19981 tggtttccag cgcgatggtc gaactttgct gcccactcac cagcggctga ccgtttttca
    20041 gggtaacgtt gtagttgccc tggtcgtcga tattgacctg cacatccatc atcccgctca
    20101 gttcttccac catctggtcg cgggcgtcgt acagcgcgga ggcgttatcg ccattggctt
    20161 cagcctgagc gatttgctgg ttatagctgg cgatgccgct ggtgagcgta ttgatttgcg
    20221 acaccatcgc ctgctgctgg ctgatgattt ccgtgctttg cgagtcgatg taatccagcg
    20281 tgttatcaat acgcaacgac agcgccccgg cttcactgat cacctgctcg cgcagggcgg
    20341 aatcatcggg gctggtggtt gcttcgttaa gggcggcgaa gaagttatcg aagccgccgc
    20401 tcaggctgct gttatcgtcg ctcagtaccg cttccagttg gctgagatcc ccctgctggg
    20461 tgctgtaata gccgtaatcg ctggcggcat accacacctg attcacctga tactggttgg
    20521 agacgcggcg aatgctgtcg acctgtacgc cgttaccggc gctgttcggc gaaccgccgc
    20581 tggcaccgat ggtgctgatc tccgccacct gacgggtgta gcccgtggtc atggcgttgg
    20641 cggtgttttg cgccgtgacg ttaagctcca cctgcgcggt tgatgcaccg ctgtagccga
    20701 tgttaatcat gtccatgttg gatccttacc ggtaaatcgt gccggttaaa agcggcgtaa
    20761 agttggggat tcttaagttc cgtaaaccgg gtgccgaacg ccggatgcgg cgtggacgcc
    20821 ttatccggcc tacatacggg cgactcgtag gcctgataag acgcgacagc gtcgcatcgt
    20881 gcaccgaacg ccgaagctca tttatgcttc ggcgacaact gcttcacaat catcgccgca
    20941 atgccaatcc cgtgctgctg cgccagggtg ttacacaact catcgtcata aaacccctgc
    21001 atcatgcgca ccgaatcgct gttaaacggg ttatcttttg aatcaaacaa ctcgttggtt
    21061 ttccgcatct ctttcagcat gttgcgtaaa aaaatcgcct caaactgttc ggcggcctgc
    21121 ttgatatcat tggcctgaac cttcggcccc attaacgcgt cgctgccgtc aatcccgccg
    21181 ctggcgttca ctttcatcag atcacctcca ggtcggcatc cagcgcgccc gcctcatgca
    21241 gcgcctgcaa aatcgacata atgtcgtccg gcgtcgcgcc taaactgttc agcgcattga
    21301 cgagcgtttt caggctgctc gactccggca aactcaccac gcccggacgg gcatgattca
    21361 ccgcgatatt gctctgcggc gtaaccgccg tgcgcccacc cgcaaaggca ttcggctggc
    21421 tgacgttgct ggtttcgtta atcgaaacgg tcaggctgcc gtgcgacacc gccgccgcgt
    21481 gtagcgcaac gccatcgccc attaccaccg tgccggtacg cgagttaaac accacgcgag
    21541 cgcggatttt ctccgcctgc acctgcacat cgtccagttg cgacataaat gccacccgcg
    21601 cgccggggct ggtgggcgcg cgcacggtaa cgttggtgga actctgcgcg gtggcaatac
    21661 cgccaaacga ggcgttgatc gccgccgcaa tgttgttggc gtctttaaac gacgggcgtt
    21721 tcaggttgag ggtgatggta tcgcccatct gaaaatcgct ggggatctcg cgctccaccg
    21781 ttgcgccgtt aggaattaac ccggcggttg gcgtattaac cgttacgctg gaaccgctgg
    21841 ccccggtggc gttcatcccg cccaccacca cgctgccctg tgccagggcg taaacttccc
    21901 catcagcgcc gtgcagttgg gtcagcagca aagtgccgcc gcgaatactt ttggcatcgc
    21961 caatcgacga aacggtgaca tcaatggtct gcccgcgcga gtacatcggc ggcagcgtgg
    22021 cgctcaccgc caccgcggcg acgtttttca ccttcgggtc gattttattc ggcagttgca
    22081 cgccaaactg gcgcagcata ttggtgatgg tctggttggt gaatttgacc tggtttttat
    22141 caccggtgcc gtcgaggccg accaccaggc tatagcccac cagttggttt tcgcgcacgc
    22201 cctgtacgtt gaccagcgac tccagcgatt gcgccagcgc cacaccaggc agcgccaggc
    22261 tcacggcgat aactgcggcc tttatccagt tttgcattgc actctccggc attaaatcgg
    22321 gaacagagga tggttaaaca gccgcgtcag ccagcctgcg gcgttggcgt cgcttaacgc
    22381 gccgcgtccg gcataggaga tgcaggcatc ggcgatccgc tgggaggaaa ccgagttgtc
    22441 gttctggatg tcgtcggcgc gcaccaggcc gcttaagcgg atgtactcat cgccctggtt
    22501 gagggtgagc catttctcgc cgcgaatttc caggatgccg ttgggctgca ccgcatggac
    22561 ggaaacggtg atctccccgc gcaggctgtt ctgctgctgg gaagtggcgc tgccgtcaaa
    22621 gtcgcggttg gcgtcgatag agccggaaag cttatcttta gtgtgactaa aaatggtcgg
    22681 tgcgccgata tcgacggtgt tgcttttgcc gaagttggtt ttcgcctgtt tgctcgactg
    22741 ggtggattct tcgaggatca ccgtcaggat atcgcccacg cgataagccc gccgatcggc
    22801 ggtcagcgac cagttgtagc cagtttcaaa tacaccgcca gcacgcccgt ctgccggagg
    22861 cggtgcttcg gttttcggtg gcgcaaagta tgcgtcgtct tttttgacca gaatggcctg
    22921 cgattcacac ccgctaagca ggggaagcag cgccaccagc cagagataat ttttcacgtt
    22981 ctgcctgttt aaatacagtg agttatgcgc ttttgtaggt cggataagac gcgtcagcgt
    23041 cgcatccgac atttcggcct tacagcgtct gactgatgta ctgcaacata tcgtccgcag
    23101 ccgacaccat tttggcgttc atctcgtaag cccgttgcac tgtaatcatc gccaccattt
    23161 cgttgacgat atctacgttc gagccttcga gggcattgtc ctgcaaagtg ccaagcccat
    23221 cttcgcccgg cacaccttcg gtcggctgac cgctggcggc ggtttccaga tacaggttgt
    23281 caccctcagc ggaaagccca gccgggttag cgaagttaac cagcgtcagt tgccctaact
    23341 cggtcacgtc gctgtcgcca ggaagaatcg ccgtcaccgt gccgtcctca ccaaacgcaa
    23401 cattcgtcgc ccccgccgga acgtcgattt ccggctgcaa tggcaagccc tgtgagttgg
    23461 tcagtacgcc atcggcgttc acctgcaaat tgccgtcgcg ggtataggcg atatcgccgt
    23521 tggccttttg cacctgcaaa aatccctgcc ccataatggc gacattcatc gcgttgtcgg
    23581 tggtttcgac gttgccttcg gtgaaggttt tctgggtgcc gacaatgcgc acgccgctac
    23641 cgaattgcag cccggtcggc atgatgttgt tctggtcgag catcgcgccc ggcgcttcct
    23701 gggtctggta gaacagatcc tgaaacatca cccggtcacg cttgaagccg gtggtgttaa
    23761 cgttggcgat gttgttggca atcgcgctca tttcagcatc ctgcgccgag aggccggttt
    23821 tgctgatcca taatgctgcg ttcatggtgg taaattccgt gttatttttg ggccgcggtt
    23881 gccggatgcg gcgtgaacgc cttatccggc ctacgatgtg cggttacgag ccgcgcaata
    23941 agcggttgcc cgcgtcgctg atatcttcgg cggttttcat catcttgatc tgtgcttcga
    24001 actggcggtt catggcgatg gacgacatca tttcgcttac cgccgagacg ttgctgccct
    24061 cgagaaaacc gccgctgact ttgatgtttt catcgcgctg ggccggaacg ccgtcggcag
    24121 tcaccagcat cccttcagca tttttctcca ggttagccac cgggatatcc accagcttca
    24181 ggcgatcgat atccatcgtc gcggtcacat cgccgtcatc gggcgtcacc gacagcgtgc
    24241 cgtcgctgcc aaacgaagca atggcgttcg gcggcagaat gatcggcccg ttatccccca
    24301 gcaccagatt gccgtcgatg gtcagttgcc cctggtcgtc ctgctgaatg ttgccgttgc
    24361 gggtatagac ttcgttaccg tttttatcct gcaccgcaat gtaacccgcg ccctgaatcg
    24421 ccacgtccag cgggcgttcg gttttctccg caacacccgt gctgtcgttc acgccgctgg
    24481 ccccttcctg cgccatatag cgcgtgtcga agccgccgcc tttcaccttg tcgttggttg
    24541 ccattgccat atcggcgcga aaaccattgg tgttgacgtt cgccaggttg ttggcgctaa
    24601 tttgctgctc atacagggtc tgagaagcac cgctcagggc ggtataaatc aggcggtcca
    24661 ttacattgcc tggaacagcg cgtcatcgag ctgcgtgctg gtggcgataa ctttggtgtt
    24721 ggcctgatag ttacgctggg cggtcatcag gttgaccagt tcgctggtga tatcgacgtt
    24781 agagctttcg agcacgcccg acgacagcgt accgcaggtg ccggagccgg gaacgccaat
    24841 cagcggggtg ccggattcgc ccgtttgtac ccatgcggtg ccgctcactg cctccaggcc
    24901 gttttcattc gggaaagtcg ccagcaccac ctgcccctgc aacatgcgct cgccgttgct
    24961 gtaggtggcg taaactttgc catcgtcatc aacctgaaca ccgttttgcg tggcggaggc
    25021 gtagccgttg gcggcgttgg tggtgaccga aaattcagag ccgtattgcg tacaggtgga
    25081 gtaatcgacg gttaagtcga cgggcgcggc ggcgtcggtt tgaaattcaa tggtctgcgg
    25141 cgtggttgga gaggtcagct tcccggtgtt cggatcgaag gttaacgtcg tcgccggaac
    25201 gccggtctgc tgctgaccgt cgaaggtgta ctgcacttcc caggtgttgt cgctggtttt
    25261 ggtgaagtac tggcataccg aatgttcgtt gcctaatgag tcatacaccg tggtggtgta
    25321 gctgtcggtg tacgaactgc tgttagtcgc gtcgaacgga acggtggtgc gatcgatagc
    25381 ttcatcgctg gcgttgaagt tagcggtaaa ggtcaggctg ctgctggcct gcgccgggat
    25441 attgccggtt ttgatctgga tatcggtgac cgtaccggtt tgcagagtgc cagtgtcatc
    25501 gaccggatag ccctgcaaat aagcgcccga ggcgttgacg atatagccgt ttttgtcggt
    25561 ttcaaacgat ccggcgcggg tataggagat attgcctgcg ctgtcacaca tgacaaaaaa
    25621 tccgtcgtcg ttaatcgcta aatcgagcgc attgccggtg gagaccagcg aaccgccacg
    25681 cgaaatactt tgcgcggtgc ccgccacgct gacgcccatc gcctggcttc cggcatacat
    25741 ggcggaaaac tgggtggtca tcgacttata gcccaccgtt ccggcgttag cgatgttgtt
    25801 actgatcccg tccagctgtt cgttaacggc attcagcccc gttgcggcaa tttcataact
    25861 cattgtgtct tcctgttaat tgattcttac gccacatcac tgtcggcatc gggtacgcca
    25921 aactggctaa tcatggtgaa cggcacttcg cccacgcctg caacgttaag caccggggaa
    25981 ctgccgtcga gcgggatgcg cacatcttcc acttcgccgg agacttcaat cggcacctct
    26041 tcttccccgg tattggtcac caccgagacg tcgtaatcgc ccggcgggat gccgtaatcc
    26101 gcgggattca ccgagaaatc aacctggcct ggtccgacgg acgaactgcc ttctgggatc
    26161 agcgagacgg tgtagtcatc gcctgcggca tcggtaaagt ggagatcggc ggtggtacag
    26221 gcgtcgtcca gcgtagcgcg accgttaatg gtctggtcgc tcacctgcaa ggcggtggtc
    26281 tgcaccataa tgtcgtcgcc caccaggtta ccgagcgcca tcacctgaat gttgctcatc
    26341 agcaccgcgt tggtgttggc ctgcacgctc atctcttcca tcatcgccac ctgcgccatc
    26401 gacgacagct ggtcgatgta ttcggtagcg tcggtcgggt cggtcgggtc ctggttttgg
    26461 atctcggcga caaacagcgt caggaaggta tcgacaggcg agctatcgct gctgctttgc
    26521 gtgtcgctgg ccgccacatt attgctttcc gccgtggcgg tcggctgcgg cgattgcgca
    26581 ttcagcgcca gggtattcat caggcttctc ccagtttcag caaactttgc tgcatacttt
    26641 tcacgttgtt gagcacatcg acgttggttt cgaaatcgcg cgaggccgac atcatgtccg
    26701 ccatctgctc caccacgttg acgtcgggat accagacgtc accgttggcg tcggcgagcg
    26761 gagaatgcgg ttcatagcgt ttcaccgccc cgccggtttg cagcacgtcc tgtacctgca
    26821 cgctggcacc gtcaatggca tgacgatgtg tacccgccag caggctgtgg tgatacaccg
    26881 cggcaaacac cgggctgcgc gctttatacg cctgcgcctc gctgctggcg ggcgcattgg
    26941 cattcgccag gttactggca acggtattca gacgtagcgt ttgcgccgtc atcgccgagc
    27001 cggaaatctg atagatatca gtaaatgaca tgcttatttc ccttcaatgg cctctctgat
    27061 gccgctgatt tgcagattca gaaaggtcaa actgctttga taatccatac tattttgtga
    27121 aaaccgcgcc tgctcgctgt tcagttccac ggtgttgcca tcttccgacg gctgcatcgg
    27181 cacgcggtac tggacatcca ccgccgcgtt atttgcccgt tgcatctccc tggcaaaatc
    27241 aatatcttta gccttgaaat ttggcgtatc gacgttcgcc agattcgcag tcagtagctc
    27301 ggtcctctca agacgtaact tcactgcctg cggatgtact cccaatgcct gctgaaaact
    27361 gatccccatt tcctgcatca ctcctgcttc aatccgcata cgctaagcaa tcgccatgcc
    27421 attttttaag tcactgatga gaaagggttt atgagttttc aattttgcag gcggaagttt
    27481 tacttcctcc tggcgctggc cttaccgggc tacgccgccg tccatcccgt gcaacacagc
    27541 gcgcgggaac aagtcaacgc ccaggtgctg aatgccgcga gccaaaaaat tgaatcgctg
    27601 gcgcagcagc ggcagtggca tgactatcgc tacaccttta agatctacat tccctcgcag
    27661 attgcgaccg ccgcgccctg tactaaaaca cccggcgtca cgctgacttc gcctgcggaa
    27721 atcgccctca accggatgaa cttcaccgtt agttgcccgc aaagctggca gatgaacgtg
    27781 gcggtgcgcc cggatgtact ggtgccggtg gtaatgacga agtcactggt tgcccgcgat
    27841 atgccgctca ccgcgaatga tgtggagctt aagccctaca acgtcagcgc ccagcgccgc
    27901 gatgtgttga tggagcttga tgacgcgatt ggtttaagca gtaagcacgc attgcaacca
    27961 ggcagaccca tcaccaaaga agaactggtg tctccggtgc tggtcgggcg cgatcagccg
    28021 gtgatgattg tttaccagag cgcggggatc accgcctcga tgcccggcgt ggcgctgaaa
    28081 aatggtcgta agggcgagat ggtgaaagtg cgcaatgcca gcagccagcg ggtcatcagc
    28141 gcgatggtgg cggaaagcgg cgtggtgaca acggtaagcg cggagtaatt tgcgcaggcg
    28201 attaaagttt tgccgcctgt taaccgcttt accgcagtaa cccacccaaa taccgagacg
    28261 aggttgcgat gaaaatcacc ccgactatgc ctggcaatcg cccgacagcc acaacggcag
    28321 gctccagcca gccgcgcaaa accacggtga ccaccaccac gacgctgagc gccgacgaca
    28381 ttactcaggc gggattgcag tctgcccagc aaacgctgaa cgaccagcaa gagagcgata
    28441 tcgacagcaa caaagtggcg caagtgcagg cgatgctggc gtccggcagc ccgcaagtgg
    28501 acactgaaca gttagccagc gacatgctga gttttttcca gaactaagag gccgatgaag
    28561 tgagtatccg gttacaacag gtgaaggcgt tgttgcaggg cattcgtgac gatgacgcgc
    28621 tgtatgattc cctgcgcgaa cttctccagc gtcagcggat ctgcatgatt cgccgcgcca
    28681 gcgaagagtt gctggcggta aacgatgaga taacccatca ctatgagcag ttgcatggac
    28741 atagccatca gcggcatagc ctgctgaaaa tgctcggtgt tagcgtaaat cgtgacggtc
    28801 tggcgcaggt tttcacctgg cttccggctg ttcaaaaagc ggctgcacaa cagttgtggc
    28861 aacgccttga gcaaaaagcg gaacgctgca aagcctacaa cgacaaaaac ggcgagctgt
    28921 tgattcgcca gtatgaattt attcagtcgt ttttaggcag cgaagcagat tttctctatc
    28981 aggaataacg cgaatacctt ccccatagcg gaaatggatt ttccgcctga tttccaatcc
    29041 cttgttttta attacaaaaa tccccggcac gcttattgca tatcctgcac agcattaaag
    29101 actggaagcc acctatgagt tttattgaat gttacgaacc ggaatacgtg cgcaaatttt
    29161 tagcgcgtcc ccccggttcg cctttatacg tccgcaaggt gtggaaaaac gagattcgcc
    29221 tgagcctgtc attgaccgat attgccagct gcgaagcggt gctcaacgat gtcagggcct
    29281 ttgatttaca gctcacctac cgccctgtgg aagataattc cgcagagctt tccgcgcgcg
    29341 atgcgatagt aaaccaggtg attttgagta ccctcacgct ccgtgattta accccggagc
    29401 tttccctgta tgctgtcggg attttgctct cctgcgccag caaaatgccg ggcagagacg
    29461 gagacatcct ggcccgtttg acgacgctgc cacaggcgct ggccgatcat gcgaaaaaag
    29521 gtacgctaca ggcacagtac gcccagctcc cgcccgtgcc acagctggcg cgccagttga
    29581 tgacgctgct cggcggttgt gcgtttgact ggtcgatttt gccggaaagc ccgcgcaaag
    29641 cctcgctgcc gttgcaggtt actttactga cgctgcatga cgccaacagc gaagccctgt
    29701 tacaacagca gcttcagacg cagtggcaaa ccacctggca acagcatttt gccaccgcgc
    29761 cctggatgat gcgtaactgg ctgatttacc gcgtttacca cgatatgatt gggcagactg
    29821 acggggcgga ttatttcccg ctggtgtgcg atttttatct cctccgcacg ttaatcagtt
    29881 tatggacgct ggatggttct ccgctgcggc aggaggatat tttcgccctc tttgccatgt
    29941 ttgaacgctg gcgagccagt gaaaatgcgc tgttggttcg ccagcaaatt cagtcgttat
    30001 gtgccgccga cccgctgctt tcagcgtttt cgctgctaac gtgagtcgct ctgcgcgcgc
    30061 tttgcccctc accctaaccc tctccccaaa ggggcgaggg gaccgatcga gcacggtgtt
    30121 aaaatcggcg ctgattttct ccctctccct gtgggagagg gtcggggtga gggcatcagc
    30181 gcgcacgttc atcacattca cacattaact cacccaaaca ccatgccaac accaaccatc
    30241 atcaaaacac tcgccaccca ccgcgaaaaa ccgtttgtca gcgtcgtcac gccaacctgg
    30301 aaccgcggcg cattcctgcc gtacctgctc tatatgtacc gctatcagga ttacccggca
    30361 gaccgccgcg aactcatcat cctcgacgat tccccacaaa gccatcaaca catcatcgac
    30421 cgcctgacca atggcacgcc ggaagccttt aacatccgct acatccatca cccggaaaaa
    30481 ctgccgctgg gcaaaaaacg caacatgctc aacgaactgg cgcgcggcga atacatcctc
    30541 tgcatggatg atgacgacta ctatccggca gataaaatct cttacaccat cgctatgatg
    30601 caaaaacacc gggcgctcat ctccggttcc gaccagatcc ccatctggta cagccatatc
    30661 aaccgtatct ttcaaagccg cagctttggc ccacataata tcctcaacgg caccttttgc
    30721 tatcaccgta actacctgaa aaagcaccgt tacgacgatg attgcaattt aggcgaagag
    30781 aaaagtttca ccgacaattt cagcgttaac ccgctgcaac tgcccggcga gcgcacgatt
    30841 ttgtgtatct cgcatagcca caacaccttt gataaagact ttattatcgg ggccagcacg
    30901 ccggtaaaca cggcgcttac ggatatcgtc cgcgatccgc tgctgcgcaa tgcttatctg
    30961 agtctgcata acgccactca tcaccaggcg atcaaccatc aggcgatcga tcaggtggtc
    31021 gtcgtgaatc tggataaacg cccggatcgc ttacaacaga tccgtgaaga actggcattg
    31081 ctgcatattc cacccgagaa gatcacccgc cttgccgcca gtgaagatga aaacggtcaa
    31141 cggggccgcc gtcagtcgca cttgcaggcg ctgcgtctgg cgcaacagca cggctggcaa
    31201 aactatctgt tactggaaga tgacgcggtt attctcaagc aggagaaaca tattcaggta
    31261 ctgaacacgc tgctggcgag cctggcgaaa attccctggc aggtgatgat cctcggcggc
    31321 gaaatttcgc aaggaacgat gctaaaaagt ttgcctgggc tggtgcacgc gcgggactgc
    31381 cgcaaagtgt gcgcttatct ggtgaacagc cgctactacc cgcaactggc gcagcagatg
    31441 agcaacgatg agcactcgct ggaagactgc tggcaaccgc tgctacgcgc cgacaaatgg
    31501 ctggcctgtt acccgagcct ttgttatcag cgaccgggct ttagcgatat ccagaaaaaa
    31561 acgactgata atattgctcg ctatttcaat aagttacccg taaccaccaa gccatccgcg
    31621 ttacccatcg ccgacaccat cggctttttt atggaaacct cattccacta cgcgctgtac
    31681 cgcccaatca tcaccgccct acaggcgcaa gggctgagct gcacgctggt gatcaacgac
    31741 cgcgtgttta aaccgtttct ggatgagatg ctggaaacac tgaaaaacat cgacgatccg
    31801 cagcttaaag gtatgcgtct gtcagaaatg cagacacatg gtcagcgggt gaaatgcctg
    31861 gtcagcccct accacacgcc tgcgctcaat ggcctggcgg cggtgaacat ccgcgctatg
    31921 tacggcttag ccaaagagac gtggaaccac gccgactgga atcgttttta ccaaagcatt
    31981 ttgtgttata gccactacag tcagcaggcg cttgcgcatt tcggcagcgc aaaagtggtc
    32041 ggcaacccgc gcttcgacgc ctggcataac ggcacgttcg atcgcgcgtt gccggaaaac
    32101 atccaatcag attaccgtaa acctacggtg ctctatgccc caacgtttgg cgcattaagc
    32161 tccctgcccc actgggctga aaaattgggc cgcttgagcg gtgatgtgaa cctgatttgc
    32221 aaactgcacc atggcacctg ctcacgcccg gaagaagcag catcgctggc cctggtgcgc
    32281 aggcatttaa aacagcgcac cgactccgcc cgccacacgc tggcgctgct ggcaaaagcc
    32341 gattacgtgc tgaccgataa cagcgggttt attttcgatg ctatccacgt tgataagcgg
    32401 gtgatcctgc tcgactttcc gggcatgaat gatttgctcg acggtgagaa aagttattcc
    32461 acggcagaga gcgccgacca gcgaatccgc gaaatattgc cggttgctca tgatgttgca
    32521 gaactgcgtt atttgttgtc agaggcgttt gactgggggt cagtgcaggc gcggcttacg
    32581 gagattcgcc atcactattg cgatgcgttt atggatggca aggcgggaga acgggcggcg
    32641 attgtgattg tggaggcgct ggggtaaacc gcgcaactgc ctgatgcgct tctcttatca
    32701 ggcctacgga tcgatcacgt cctgtaggcc ggataaggcg tttacgccgc atccggcaag
    32761 aaaatacatc tctgttttag tactttataa aagccctcta atcacctcaa taatccccgt
    32821 cgtcgacacc gacggcgtgc gggggaaata gaccacctca caaatatacg caaagctgtc
    32881 aaacttccct gcccagtcat cccccatcac taaaatatcc gcactgtacc cgcgcaaata
    32941 ctccgctttc tgctctagcg attcttccag aaatacgcca tccacgcacg ccagcccggc
    33001 gatgatcgcc atgcggtcat cctgatggta aaccggtgcc ctgccctttt tggcgatgtt
    33061 cagggcgtcg gacgatacgc cgaccagcag ccgctcgccc aatgcgcggg cgcgttgtaa
    33121 aaggcgcaga tgaccgacat gaaacacatc gaaggtgccg aaggtgatga ccgttttcat
    33181 ggctactccg ctaatttgtg ccgcagccag cactgcacgg cgatgtcgtc caccgctttt
    33241 tgcgctttcg ccgtggcgct ttcttgttgc tcgcggcgtt tgtcgtccag cgtcagggcg
    33301 actactttct cttcgcacgc cgccgccgtg aggtttttct gcattcgcgt ggctttgata
    33361 ttggccagcg ttttttcctg ctcctgccag gcgattacct tgcgcagggt gcctttgtag
    33421 ccgctgacgt ttttcagcga ttccaccgac accgccgcac ccgcagatgt tttctcgaca
    33481 aagtagccta acgccttgat attgttgtca taacccgcgc aaatctgttt ttgctttgcc
    33541 agctcgacgg ttttgtcttt gaccgattta tcccgcaggc gctgcaactg cgtcagggtg
    33601 tcgatgattt gtcgcatagt tactgttctg cctttttgac accaggaaag agaatttgca
    33661 ggcggctttg caccagttcg agggaggcgg gttcgcgcat ctcctggcgc aaaaatcgct
    33721 cgatggccgg gaacattttt accgctttgt cgacgctggc gtcggcacct gccacataac
    33781 cgccgagcgg gatcagaggt ttgatctcca tataggcggc atagttttgc tttaacgcgc
    33841 gggcggattg ctgatgttcc aggctggtga cctggttcat gcagcggctg atcgactggc
    33901 cgatatcaat ggccggatag tgccccgctt ccgccagttt gcgggttaat acgatatgcc
    33961 cgtccagcac cgcgcgcgcg cagtcgacaa tcggatcttg ctggtcgtca ccttccgcca
    34021 gcacggtgta gatggcggtc atcgatcccg cgctttcgct gttgcctgcg ctttcgacca
    34081 gtttggggat catgccgaat gccgatggcg gataaccttt ggtggcgggc ggttcgccga
    34141 gcgagagggc gatttcccgc tgcgccatcg cgtagcgggt cagggaatcc accagcagta
    34201 agacgtgatg cccgcggtcg cgaaaccagg cggcgatgga gtggcacagt tcggtggctt
    34261 tcagacgcat cagcggcgat tcatcggcag gcgcgacgac gacgatagac tttgccagcc
    34321 cgtccgcgcc cagcgagtgg tcgataaact ctttcacttc gcgcccgcgt tccccaatca
    34381 gcccaaccac cacgatatcg gctttggtct ggcgggtgat catccccagc agcacgcttt
    34441 tcccgacgcc gctgcccgcc attagtccga cgcgctggcc tttgccgatg gtcagcaggc
    34501 cgttgatcgc tttcacgccc acgtccagcg gttcgtctac cgaacgacga gttaagggat
    34561 taaccgaggg cggcaacggc ggcagaaggt cgttgccgtt caggcggcct ttgccatcca
    34621 gcggttcgcc gaggccattc accacgcggc ctaaccagct ttcgcctatc aggatgtcgt
    34681 gggctttttc ttccgggaag acgcgtgcgc ctgccatcag gccgaccggc tgtttaaacg
    34741 gcattaaata ggtgatgtcg cgattaaagc ccacggcctg cgcgtcgatc agcgtgtgat
    34801 tggcactttc gatgcggcac agctgcccgg tcattagcgg gcagccgaca ctctccagca
    34861 ggatgccgtt aaggcgcacc agccgcccgg cgacgcgggc cagcgggatc gactccagcg
    34921 agcgcagggc gttatcgaac gcagaaaaat cactcaccct gcgactccgg cgtgagtgtt
    34981 tctttcagcg cggtcatgca ttgttcaagg cgatgctcgc agccgatatc cagttcggat
    35041 ttgtcggtga tcacccggca ttcgcccggt ggcaaatccg gcgaaggcgt cagcccccat
    35101 tcgctgacct tttccggcac cgcgtcgcgc aggcggttaa actcttccgt gctcagcatt
    35161 acttgcaggg tttccggcat cgccgggaag gccgccagcg cctcttccac cagcgccagc
    35221 aactgcgtgg gttgcagcgc cagttcacaa cggatcacct ggcgggtgac tttttccacc
    35281 agttgcagca aatcttcccg ctgtttacgt tcgatatgag cgaggaagtc gttcacttta
    35341 ccggtgatgg cttccagcgg ctgcgccgcc tcaacaaact gtttgcgccc ttcctgctgc
    35401 ccggcgaggc tgccttcggt gtaaccctgg cgtcgcccct cggcaaaacc ttgctgatga
    35461 ccttcgctaa agccctgctc ctggccttcg gtcattcctt gagcaaaccc tttttgcagc
    35521 ccttcctgaa atccgtccat caactggcgc tggtaatcgg cggcgctaat actggtagtg
    35581 agcggttctc cggtcaccgc acgctggcgc ggcgggaaac ggtgcaggcg atagcgcccg
    35641 cgcagtgttt caatggtcat ggttactccg ccgtctgttc cgcaaagagc tgtagctgaa
    35701 tttcgcccgc ctcttccagt tcacgggcga tgcccatgat ttcgcggcgg atctgttcga
    35761 tacggctgac cggcaccggc cccagacgcg cggtgatggc ttccagttgc tgcgcctgcc
    35821 gttttggcat taccgcgtaa atcgaacggc gcagcagggc ttcggtgcct ttcagcgcca
    35881 ccgcccagtc ttccatcggc acttcgtcaa gcagacgtcg acgcacctct tcgttctggc
    35941 ggctgaggat aaagaagtcg tacatctcat cctgaagctg ctccagcacc tcttcgtcgc
    36001 gctcgcgcat ctggtcgagg atcacctgct ggttgccctg gaagcggttg acgatatccg
    36061 ccgcctgttt gatgcctttc acttttgagc cgtgttccga aagcaccgac aggccgcgtt
    36121 cgatcagcct gtccagttca tccaccacgt cgcggttgac gtcgttgagt ttcgccaccc
    36181 ggtagagaat ttcgttttgc accgattcgt tgagatacga gagcaccgct gccgagattt
    36241 ccggcgtcag aaacgcgagg aacaccgcct gtaattgcag atgttcttcg gagatcagca
    36301 tcgccagctg gcgtggttcc acccattgca ggcgcgccat ccgcgagcgg atctcatcgc
    36361 cgtagatgcc gttgatcaca ctgctggcga tctcggtgcc gagggctttg ttgagaatac
    36421 cctgcaacat ggatcgcgat gcgccgttga tcccgctctg ttcgcggaac tcatcaaaga
    36481 agttattgat cactttgcgc gccatgctgg ttttcacgcc ggaaagccgt gccatgtttt
    36541 cgctcaggcg caccacttct tcgcggctga gtttttgcat caccgtcgcc gccgcctctt
    36601 cgcccagaca cagtagcagg atcgccgcct gttccaggta gctgttgctc ttatttgttg
    36661 tcaattcgct cattgctggt gatccattgt ctgagaactt cggctacgcg atcggtgtcg
    36721 ctcatggcca gtttttgcag gaactcgagt tttacttcca ggccggaact ctgagacggc
    36781 aggctgtcat cgccgggcag cgacggcaga tcgacgtttt tgcgctcctc ttccggcatg
    36841 gcaatccacg gttcgctgac ggcttccgat tctggtgtga taatcggtgc acgtaccgcc
    36901 gtcagccgtt tcattaccgg gcgcaccacg aagaacagca acaacagcgc cagcaggccg
    36961 cagccaatca ggcgcaccca ggccagcacg ctgtcatctt tccacagcgg aataattggt
    37021 tcgaccggca ccgcctgcgg cacaaagtta agcagtgaaa gcgtcaggtt atccccacgt
    37081 tgcgcgtcaa taccggcggc attgttcagc aacgcggtaa gctgcgcggt ctgctccggc
    37141 ttccagtttt tcagcgccgg agcgttttga ttgagcacca ccgccacatt gaggcgtttc
    37201 accgcaaagc cgggatgctg aatatgctgg acgctacggt cgtaagagta atcgcgcttg
    37261 ctttcgctgt gtttggataa cgcttcaggc gagcgatttt cttccgtacc gttagtcatt
    37321 tgattcgccg caaccggcgg acggttgctg agagagccgg gcacacccat cgccacctga
    37381 ttggtgtcgc tgtccagcac gctctcttcg cggtttattt tcggcgcgtc gccgtagtgt
    37441 tcctgagttt cgtcgatggt gctgaggtcg agatccggca tcacgctgac acggtaattc
    37501 ccgctgccga ccagcgaatc cagcacgttc gccacgctgg cgtgggtttt gtcctgaatg
    37561 tctttgagga tctgatcgcg tttacgggta gcggcagaga ccgcctcgcc cgcgccgatg
    37621 ccgtcggtca gcagatttcc cgcctgatca accacgctaa ctttgctggc gtgcagcccc
    37681 ggaatgctgc cggaaaccag atgcacaatg gcgttcacct ggtccatatt cagcttcgcg
    37741 ccgtagtgca gacgcaccac caccgaggcg ctgttttgcg gctcatcgct gaccacaaac
    37801 gagctttctt cgttgagcgc cagatgcacc cgcgcgctct ccaccgcgtc cagcgacata
    37861 atgctctgcg ccagctccct ttcgaggctg cgtttatagc gcacgttctg cacgaactgg
    37921 ctgctgccaa gcacttcgtc tttgtccatc agctcgtagc cgctgggcaa aatcgcctgc
    37981 acgcctttgg cggcaagggt catgcgcgtt ttcgacagcg cctcttccgg caccagtatc
    38041 tgcccgcttt gcgggtcaat gcggtaggtg agcttttcgc cgtccagcac ggtgacaatc
    38101 tgcgacacgg gcagattttc ctggcgtccg tagagcgaga cgtagccgcg attgccggtc
    38161 cacagcacgc taacgataat cgccgtcgcc gccacggcaa gccccacacc cgccatcagc
    38221 gcccagcgtt tgttatctgc gagacgcagt cgaaacgccg gaaacgccag ggttaacttc
    38281 tttatttgtg cattcatagg gttagatcga catactcatc agatcgttaa atccggtggc
    38341 gattttgttg cgcacctgca ccagcgcgga gaaagagagc gacgcctgct ggctggcgat
    38401 catcgcacct gccagatcat cgcttttgcc catgtcgata gccgtttgct gctgctccgc
    38461 cacctgctgg aactggtcaa cgtgaccgag cgcgccctgc ataatgcggt gaaacgaaac
    38521 gtccgggtcg gtggaactga aggtcatggc gggcagcacc ggcgcctggg cgttggcctg
    38581 catccgctgc acgtcctgca tgatctgcgc ctgcatggtg ctgttgattg tggtaatgct
    38641 catttttaac ttccgggtca ggagaaaact tcgataccgt gcttgcgcat ggaggccagt
    38701 cgatagcgca gtgcgcgcgg ggtaatacct aacaggtcgg cgattttgct gcggttgccc
    38761 tgatatttac gcatcaggtc agcgatatat tgatactggg cgcttcgtcc gtgctggcct
    38821 aaatcgctgg tttcggcaat gtgtacggcg ggctgagcag gccattgcag ttcgctgtgg
    38881 tcggcaatgt cattttccgg caggcctaag gcgtccgggt aaatcacgcc gtcgcgattt
    38941 aaaatcattc cccgctgaat ggcgttctcc agctggcgca cattgccggg ccagcggtaa
    39001 ttcagcatcg cacggcgggt agattctgaa agggtgatat ttttcaccag caccgtggag
    39061 tattttttta taaatgactc tgccagcgga ataatatcgt tcagacgttc acgcagcggc
    39121 ggcatggtaa taggaataac cgccagacga taatagagat cttcacgaaa acgcccggcg
    39181 gcgacttcct gctcaaggtt tttattggtg caggcaatca ggcgaaaatt gagtttaatt
    39241 tgtcggttgc tgcctaatcg ctcaacctgt tgttcctgca atacgcgtaa tattttggcc
    39301 tgtaacgcca gcggcatatc gccaatttca tcgagtaata aggtgccgtt attcgccagt
    39361 tccatttttc ctggcacact ggcaattgcg ccggtaaatg cgcctttgtc gtagccaaat
    39421 aaggttgctt ccagcatgtt ttccgggatc gcagcgcagt tcacgccgat atagggcgcg
    39481 ttttcatttt caccaaaggc gactgtgtga atatatttcg ccacgcactc tttgcccgcg
    39541 ccggtttcgc cctgaatgag caccggcacg ttaaatgcgg cgacacgctt tgccagagta
    39601 aaagcgttaa tgctggaagc ggcagttgca ataagttcag acatagggtg aacgatataa
    39661 gtagcgacaa taatcaggat gttctgaagc gtatcaccgg cgggattaac aagttcgaat
    39721 taaacgataa taaaaacatt atttgtttat aaacaatgat ttatccatgt aggccggata
    39781 aggcgtttac gccgcatccg gcaattgtgc accggtgcct gatgcgatgc ggcgcgtctt
    39841 atcaggccta cggttcgtgc aatgtgttat gacgatgcct gatatctcgc cctttaataa
    39901 tttaattatt ttttcaccct attaataatt tgcccctttc acgccccctc tatttaatac
    39961 ctcaccttgt gtcactgccg gatttgcgtg cacaaaatcg cccataccat tacagcagag
    40021 aaagtaagcc atgctgaagt acagtaaaac gccaggcatc tttaaattag aaggtaatcg
    40081 gctcggacgc ccctaccatc gcctgccgac cctgtttaca gggaattttg acgtgattga
    40141 ttcgcatctg ggcagctact ttctcaaaaa gcatcgcagc aatatcaccc taaaaaagat
    40201 tggctgcgaa atggatatta ttaataaaaa cgctgaactg atggtttccc aggtcgggca
    40261 tctggcgttt gatattgatc gctctttgct gttaatgctg ctgggcaatt tttatggtct
    40321 ggagtcctct cttgaagagg cgaaagcgca taacgggctg ccgaccaaaa ccgaaacgcg
    40381 gttaaaaaac cgtctggcgc tggatatctg cacgctgatt tttaatctgc aaacctccgg
    40441 tattgcgctg aaattaaagc tggacagcag cacggtaatt acccactggg cgtatcaatt
    40501 aacctttacc cttgcgggcg atgaagagag ctgttttcgt attttgctcg acgacgccca
    40561 caccgatttt attttaaatc tgatccgcca cagcgagcac agtaaaccgc agcaggtggc
    40621 gaaatcggtc aataaaccgg ccctgattaa agagatcatc cgctccctgc cgctgaccct
    40681 gaacgtcaaa atcgccgagc tatcgatgaa tgtcgcggat ctcacgcaga taaaagccgg
    40741 ggacatttta cccatctcgc tgggagaaac gttcccggtg gcgattgggc aatctgaatt
    40801 atttagcgcc ctgattgtgg aagacaaaga caaactgttt ttgtcggagc tggcgggcaa
    40861 aaatgagaat agccatgagt aagcaagaag atattttagc cggggatttt ggcctcaccg
    40921 atgaagttgc gcctgtcgca aaatccgccg acgctgaaac gctggtcacc cgcctggaag
    40981 accgtttttc cgactcgatg acgctgctaa aacgcattcc ggtgacctta acgctggaag
    41041 tgtcatcagt agaaataatg ttagccgacc tccttaacat cgacgacgac acggtgattg
    41101 agctggataa actcgccggg gagccgctgg atatcaaagt gaacaatatc ctgctgggca
    41161 aagcggaagt ggtggtggtc aacgagaagt acggcctgcg cgtgctggaa tttaacaccc
    41221 gcgatatcaa cgacctggca ccatgaaacg ccgcactcaa ctgacgctgg gccttgggct
    41281 gctggcgctt gcgccggtgg cgttggccca gggcggcgat attgcgctgc ttaacgtggt
    41341 cacccacggc aacactcagg agtacagcgt caaaatccag gtgctgattc tgatgaccct
    41401 cgtcggtttg ctgcctacga tggtgctgat gatgacctgc tttacgcgct ttattatcgt
    41461 gctgtcgctg ctgcgccagg cgctagggct acaacaaacg ccgccaaacc gcattctgat
    41521 tggcattgcg ctctccctca ccatgctggt gatgcgcccc atctggctca atatttacga
    41581 ccacgcagta gtgccgtttg aaaatgatca gattaccctg accgatgcgt taagcaccgc
    41641 cgcgacgcca ctcaaacgct ttatgctggc gcagactgac aaaaaagcga tggcgcaaat
    41701 catgactatc ggcggcgcga agggccatgc cgccgaccag gatctctcga ttgtggtgcc
    41761 cgcctatgtg ttgagcgagc tgaaaaccgc cttccagatt ggctttatga tttacatccc
    41821 gtttctggtt atcgacctga ttgtcgccag cgtactgatg gcgatgggga tgatgatgct
    41881 gtcgccgctg atcgtttcgc taccctttaa gctgatgctg tttgtgctga tcgacggctg
    41941 gtcgctgacc atcggtacac tcaccaccag cattcgcggg ctggggctgg gctgatgatg
    42001 ctgaccatag acgtagccgc cgacattgtc gccagcggca taaaagtggt gattttgctg
    42061 gtttcggtgc tggtagtgcc gagcctgctg gtcggcctgc tggtgagcgt gttccaggcg
    42121 gtaacgcaga ttaacgaaca gaccctaagc tttttgccga ggctgattgt cacgctggtg
    42181 gtgctgggcg tgtgcggtaa atggatgatt atccagttgc atgatctctg cattcacctg
    42241 tttacccagg ccgcgttgct ggttcattga gatgcgcaca agcgacgtta cgcaactgat
    42301 ggatctggcg ctggggctgt ggtttccctt tgtgcgcatt atggcgtttc tgcgctatgt
    42361 gcctgtgctg gataacagcg ccctgaccgt gcgcgtgcgg attattttgt cgctggcgct
    42421 ggcaatcatc atcacgccgt tgatccccca tcctgttccc cacgacctga tatcgctcaa
    42481 cagcctgatc ctgacggtgg agcaaatttt atggggcatg ttattcgggc tgatgtttca
    42541 gtttttgttt ctggcgctac agctggccgg gcaaattctc tcgttcaata tggggatgag
    42601 catggcggtg atgaacgacc cgagcagtgg cgcatcgacc accgtgctgg cggagctcat
    42661 caacgtctac gcgatcctgc tgttttttgc catggacggg catctgctgc tggtgagtgt
    42721 gctgtataag ggctttacct actggccgat tggcaacgcg ctgcatccgc aaaccctgcg
    42781 caccatcgcg ctggccttta gctgggtgct ggcgtccgca tcgctgcagg cgctgccaac
    42841 cacttttatc atgctgattg tgcagggctg ctttggcctg ctcaaccgca ttgcgccgcc
    42901 gctgaacctt ttttcactcg gttttcccat caacatgctg gcggggctgg tctgctttgc
    42961 caccctgctc tacaacctgc cggatcacta tctgcatctg gcgaattttg tgttgcagca
    43021 actcgacgcg ctgaaaggtc actatggcgg atagcagcag cgaagaaaaa acagagaaac
    43081 cctcggcgca aaagctgcgt aaagcgcgcg aagaggggca actgccgcgc tcgaaggata
    43141 tggggctggc ggcgagcctg tttgcggcat ttgtggtgat ctccagcagc ttcccgtggt
    43201 atgccgattt tgtgcgcgag agttttatca gcgtgcacca gtatgcacag gagattaata
    43261 acccagaggt gatcggccag tttctgcgcc atcacctgtt aattctcggc aagtttattc
    43321 tcaccttgct gccgatgccc gccgccgcgc tactctcctc actagtgccc ggcggctggc
    43381 tgtttctgcc taaaaaaatc ctcccggatt tcagcaaaat cagcccgtta aaaggcattg
    43441 agcggctgtt ttccagtgaa catctggcgg aaacgggaaa gatgacggtg aagtcggtgg
    43501 tggtgctggt gatgctgtgg gtaagcctgc gcaataactt tgcctccttt ctcggcctgc
    43561 aggcgctacc gttcaaactg gcgatgaatg agggtttatc gctctacgcc tgcgtgatgc
    43621 gcaactttgt catcctgttt atcttctttg cgttaatcga cgtgccgctg gcgaaagccc
    43681 tgtttaccaa agggctgaag atgaccaaac aggagctgaa agaagagtat aaaaaccagg
    43741 aaggcaagcc ggaagtgaag gcgcgcgtgc ggcgtctgca acggcagttg gcgatggggc
    43801 aaattcgcaa agtggtgccg aaagccaacg tggtgatcac caacccgacc cactacgccg
    43861 tggcgttgca gtatgactcg tcgcgcgccg ccgcgccgtt tgtggtggca aaaggcaccg
    43921 atgaaatcgc cctctatatt cgccaggtcg ccgctgagaa tcaggtggaa gtggtcgagt
    43981 tcccgcgact ggcgcgctcg gtttactaca ccacccaggt gaaccagcaa attccgtttc
    44041 agctttaccg ggccatcgcc cacgttctga cctacgtcct gcaaatgaaa cactggcgcg
    44101 agggtgcaca gccgcgcccg gccctgaaca gacatatttc cattcccaaa gaggtgctta
    44161 aactggatgg cgaaaacaac taaatcgttc ctcgcgctgc tgcgcggcgg caatcttggc
    44221 gtgccgctgg tgatcctgtg tattctggcg atggttattt tgccgctgcc gcctgcgctg
    44281 ctggatattc tgttcacctt caacattgtg ctggcggtga tggtgctgct ggtggcggtg
    44341 tcggcgaaac gaccgctgga attcagcctg ttcccgacca ttttgctgat caccacctta
    44401 atgcgcctga cgctcaacgt cgcctctacg cgcgtggtgc tgctgcacgg gcatctcggc
    44461 gcggacgcgg cgggtaaggt gattgagtcg tttggtcagg tggtgatcgg cggcaacttt
    44521 gttgtcggct ttgtggtgtt tatcatcctg atgatcatca actttattgt cgtcaccaaa
    44581 ggggccgagc gtatttccga ggtttcagcc cgctttaccc tggatgcgat gcccggtaag
    44641 cagatggcga ttgacgccga tcttaatgcc gggttgatca atcaggcgca ggcacaaacc
    44701 cgacgtaaag atgttgccag cgaggccgat ttctacggcg cgatggacgg ggcatcgaaa
    44761 tttgtgcgcg gggatgctat cgccgggatg atgattctgg cgatcaacct gatcggcggc
    44821 gtctgtatcg ggatcttcaa atacaacctg agcgccgacg ccgccttcca gcagtatgtg
    44881 ctgatgacca tcggcgacgg gctggtggcg caaatccctt ccctgctact ctccaccgcg
    44941 gcggcgatta tcgtcacccg cgtcagcgac aacggcgata tcgcccatga cgtgcgtcac
    45001 cagttgctgg caagcccatc ggtgctctac accgctaccg gaattatgtt cgtactggcg
    45061 gtagtgcctg gaatgccaca tctgccgttt ttgctgttca gcgccctgct tggctttacc
    45121 ggctggcgga tgagcaaacg cccgcaggcg gcggaagcgg aagagaaaag tctcgaaacg
    45181 ctgacccgca ccatcaccga aaccagcgag caacaggtca gttgggaaac cattccgctg
    45241 atcgagccca tcagcttaag cctcggctac aagctggtgg cgctggtgga caaagcccag
    45301 ggcaacccgc tcacccagcg tattcgcggc gtgcgacagg tgatatccga cggcaacggc
    45361 gtgctgctgc cggagatccg cattcgggaa aacttccgcc ttaagcccag ccagtacgct
    45421 attttcatca acggcattaa ggctgatgaa gcggatattc cggcggataa actgatggcg
    45481 ctgccctcca gcgaaaccta cggcgagata gacggcgtgc tggggaacga cccggcgtac
    45541 gggatgccgg tgacctggat ccagcctgcg cagaaagcga aggcgctgaa tatggggtat
    45601 caggtgatcg acagcgccag cgtgattgct acccatgtaa ataagattgt gcgcagctat
    45661 attcctgatt tatttaacta tgacgatatt acgcagttgc ataaccgttt ggcgtcgatg
    45721 gcaccgcgcc tggcggagga tttaagcgcg gtgctcaatt acagccagtt gctgaaagtg
    45781 taccgggcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat cgccaccgtg
    45841 ctggtcgcca gtagcgcggt gaccaaagat catattctgc tggcagccga tgtgcgcctg
    45901 gcgctgcggc gcagcattac ccatccgttc gttcgcaagc aggagctgac ggtgtatacc
    45961 ctgaataatg agctggaaaa tctgctgact aacctagtga atcaggcgca acagggcggt
    46021 aaagtgatgc tcgacagcgt gccagtggac ccgaatatgc tcaaccagtt ccagagtacg
    46081 atgccacagg tgaaagagca gatgaaagcg gcggggaaag acccggtgct gctggtgcca
    46141 ccgcagctgc gccctttgct ggcgcgttat gcaaggttgt ttgcgccggg gctgcatgtg
    46201 ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga ttatgggggc gttgagttga
    46261 tgggggattc ttttccatgc tttgcagcac ctgacaccat taattgacgt cacactgcaa
    46321 agagactacg atgacaccat acaatgacgt catggattga tagtatgatt aaggtcagcg
    46381 cgctaggttc tcgtcataga aaaacattcg acgatgtaat gacggagccg ccaaaatctg
    46441 gaattaaatg ggatgatgtt gttgcactca ttaaagctgt gggtggaaca ataaaaaata
    46501 acaacggctc cagacgaaaa tttcagattg gaacgaccaa attcagcaca catgagccac
    46561 atccaaaaaa tgtgatggat aaaggcgcag tagcaggtct taaggagtgg tttgttaact
    46621 gtgtaggggt agattatgaa taaagcatcg aacattatca caattaaagg tcaaccagcc
    46681 gcagtaacat ttgaagccga tatcaacgca tttcgcggca agttcctcaa tgtaaacggt
    46741 tattgtgact ttgtagccac aagtattgag gcattatatc gtgaaggcga aagcgcgctg
    46801 gatgagtgga tggcggattg tgaagaggac ggtattgccc cttttcgtga agaagaagag
    46861 caaaaacgcc tgacgcttcg tgttcctcat cacattgatt cgcggctgac catcgtcgcc
    46921 agacaacatt ccatttcaaa aaaccagctc attgttgatg tcttagaacg tgagttttca
    46981 gcccagatgt aaacaggtcg gatgccgcgt ggcatccgac cttttgccag ttcgtcaata
    47041 ttacccattt acaaaatcgt ctcctccgtc aaaatccgcc gcgcgcctaa aaagtgatcc
    47101 tgccagaaag gttcagctaa ctgacttacc cgaatggttt ccccggtgcg cggtgactca
    47161 ataaactgac cgtcgcccag atacaccccc atatggtcgg caatctcgcg gctgtggata
    47221 tggaaaaata gcaaatctcc ccggcgcagg tcgttattcg ccacaatggt tgcccggcga
    47281 tagtgataca tctcattagc cgtgcgtggg agtttcgcct caaggatctt gttgtaggca
    47341 taaaaaacta acccgctaca gtcgaagcct tgatcgggcc gcgtaccgcc ccagacgtaa
    47401 ggcttgccaa gctgttgctc cagacggtga atcgccactt cggtgatgtt atgcagatgg
    47461 tcgctgctta agaaatggtt gttttccgcc agcgcctgcc agcgtctgtc actcgccttg
    47521 agcggcgcag gaaaccattc tggatgctgt tttatctgtt cgcggttgtg ctggcgtagc
    47581 gcccttctgc tttcggcatt gccttccaca atatagctgg cctgtttttt cagatgggtt
    47641 tggtactgtt ttaataaacg cgcacgattt tgcatccgct ggcgggcggc gtagctgaaa
    47701 gtgatatgtg atgtggtggc gctggcaaat gaagaaaacg gcaagcaaag caacagtaat
    47761 cccgacaaca caaaagaggg aatcgacggc aaggacatac tcaataatgg ctgatcatgc
    47821 attgatgttg cagtgtatcc cggcccgt
//
LOCUS       DAZFKM010000020        47703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFKM010000020 DAZFKM010000000
VERSION     DAZFKM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            64..696
                     /gene="comR"
                     /locus_tag="ACHKAY_001271"
     CDS             64..696
                     /gene="comR"
                     /locus_tag="ACHKAY_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO5699980.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(758..1297)
                     /locus_tag="ACHKAY_001272"
     CDS             complement(758..1297)
                     /locus_tag="ACHKAY_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO5699981.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1524..2828)
                     /gene="ndh"
                     /locus_tag="ACHKAY_001273"
     CDS             complement(1524..2828)
                     /gene="ndh"
                     /locus_tag="ACHKAY_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO5699982.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3228..3770)
                     /gene="ycfP"
                     /locus_tag="ACHKAY_001274"
     CDS             complement(3228..3770)
                     /gene="ycfP"
                     /locus_tag="ACHKAY_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO5699983.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3793..4818)
                     /gene="nagZ"
                     /locus_tag="ACHKAY_001275"
     CDS             complement(3793..4818)
                     /gene="nagZ"
                     /locus_tag="ACHKAY_001275"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO5699984.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4829..5653)
                     /gene="thiK"
                     /locus_tag="ACHKAY_001276"
     CDS             complement(4829..5653)
                     /gene="thiK"
                     /locus_tag="ACHKAY_001276"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO5699985.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5634..6275)
                     /gene="lpoB"
                     /locus_tag="ACHKAY_001277"
     CDS             complement(5634..6275)
                     /gene="lpoB"
                     /locus_tag="ACHKAY_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO5699986.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6289..6666)
                     /locus_tag="ACHKAY_001278"
     CDS             complement(6289..6666)
                     /locus_tag="ACHKAY_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO5699987.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6669..7028)
                     /gene="hinT"
                     /locus_tag="ACHKAY_001279"
     CDS             complement(6669..7028)
                     /gene="hinT"
                     /locus_tag="ACHKAY_001279"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO5699988.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7362..9551
                     /gene="fhuE"
                     /locus_tag="ACHKAY_001280"
     CDS             7362..9551
                     /gene="fhuE"
                     /locus_tag="ACHKAY_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO5699989.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9611..11044)
                     /gene="ptsG"
                     /locus_tag="ACHKAY_001281"
     CDS             complement(9611..11044)
                     /gene="ptsG"
                     /locus_tag="ACHKAY_001281"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO5699990.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11339..12136)
                     /locus_tag="ACHKAY_001282"
     CDS             complement(11339..12136)
                     /locus_tag="ACHKAY_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5699991.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12147..13151)
                     /gene="holB"
                     /locus_tag="ACHKAY_001283"
     CDS             complement(12147..13151)
                     /gene="holB"
                     /locus_tag="ACHKAY_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO5699992.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13148..13789)
                     /gene="tmk"
                     /locus_tag="ACHKAY_001284"
     CDS             complement(13148..13789)
                     /gene="tmk"
                     /locus_tag="ACHKAY_001284"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5699993.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13779..14801)
                     /gene="yceG"
                     /locus_tag="ACHKAY_001285"
     CDS             complement(13779..14801)
                     /gene="yceG"
                     /locus_tag="ACHKAY_001285"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO5699994.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14804..15613)
                     /gene="pabC"
                     /locus_tag="ACHKAY_001286"
     CDS             complement(14804..15613)
                     /gene="pabC"
                     /locus_tag="ACHKAY_001286"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO5699995.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15733..16974)
                     /gene="fabF"
                     /locus_tag="ACHKAY_001287"
     CDS             complement(15733..16974)
                     /gene="fabF"
                     /locus_tag="ACHKAY_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO5699996.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17062..17298)
                     /gene="acpP"
                     /locus_tag="ACHKAY_001288"
     CDS             complement(17062..17298)
                     /gene="acpP"
                     /locus_tag="ACHKAY_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5699997.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17509..18243)
                     /gene="fabG"
                     /locus_tag="ACHKAY_001289"
     CDS             complement(17509..18243)
                     /gene="fabG"
                     /locus_tag="ACHKAY_001289"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO5699998.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18256..19185)
                     /gene="fabD"
                     /locus_tag="ACHKAY_001290"
     CDS             complement(18256..19185)
                     /gene="fabD"
                     /locus_tag="ACHKAY_001290"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO5699999.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19201..20154)
                     /gene="fabH"
                     /locus_tag="ACHKAY_001291"
     CDS             complement(19201..20154)
                     /gene="fabH"
                     /locus_tag="ACHKAY_001291"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO5700000.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20222..21292)
                     /gene="plsX"
                     /locus_tag="ACHKAY_001292"
     CDS             complement(20222..21292)
                     /gene="plsX"
                     /locus_tag="ACHKAY_001292"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO5700001.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21373..21546)
                     /gene="rpmF"
                     /locus_tag="ACHKAY_001293"
     CDS             complement(21373..21546)
                     /gene="rpmF"
                     /locus_tag="ACHKAY_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO5700002.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21598..22119)
                     /gene="yceD"
                     /locus_tag="ACHKAY_001294"
     CDS             complement(21598..22119)
                     /gene="yceD"
                     /locus_tag="ACHKAY_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO5700003.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22318..22902
                     /gene="yceF"
                     /locus_tag="ACHKAY_001295"
     CDS             22318..22902
                     /gene="yceF"
                     /locus_tag="ACHKAY_001295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO5700004.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23014..23973)
                     /gene="rluC"
                     /locus_tag="ACHKAY_001296"
     CDS             complement(23014..23973)
                     /gene="rluC"
                     /locus_tag="ACHKAY_001296"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO5700005.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24546..27731
                     /gene="rne"
                     /locus_tag="ACHKAY_001297"
     CDS             24546..27731
                     /gene="rne"
                     /locus_tag="ACHKAY_001297"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO5700006.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27927..28880)
                     /gene="flgL"
                     /locus_tag="ACHKAY_001298"
     CDS             complement(27927..28880)
                     /gene="flgL"
                     /locus_tag="ACHKAY_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO5700007.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28892..30535)
                     /gene="flgK"
                     /locus_tag="ACHKAY_001299"
     CDS             complement(28892..30535)
                     /gene="flgK"
                     /locus_tag="ACHKAY_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO5700008.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30601..31542)
                     /gene="flgJ"
                     /locus_tag="ACHKAY_001300"
     CDS             complement(30601..31542)
                     /gene="flgJ"
                     /locus_tag="ACHKAY_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO5700009.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31542..32639)
                     /gene="flgI"
                     /locus_tag="ACHKAY_001301"
     CDS             complement(31542..32639)
                     /gene="flgI"
                     /locus_tag="ACHKAY_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO5700010.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32651..33349)
                     /gene="flgH"
                     /locus_tag="ACHKAY_001302"
     CDS             complement(32651..33349)
                     /gene="flgH"
                     /locus_tag="ACHKAY_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO5700011.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33402..34184)
                     /gene="flgG"
                     /locus_tag="ACHKAY_001303"
     CDS             complement(33402..34184)
                     /gene="flgG"
                     /locus_tag="ACHKAY_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO5700012.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34322..35077)
                     /gene="flgF"
                     /locus_tag="ACHKAY_001304"
     CDS             complement(34322..35077)
                     /gene="flgF"
                     /locus_tag="ACHKAY_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO5700013.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35097..36302)
                     /gene="flgE"
                     /locus_tag="ACHKAY_001305"
     CDS             complement(35097..36302)
                     /gene="flgE"
                     /locus_tag="ACHKAY_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO5700014.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36327..37022)
                     /gene="flgD"
                     /locus_tag="ACHKAY_001306"
     CDS             complement(36327..37022)
                     /gene="flgD"
                     /locus_tag="ACHKAY_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO5700015.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37034..37438)
                     /gene="flgC"
                     /locus_tag="ACHKAY_001307"
     CDS             complement(37034..37438)
                     /gene="flgC"
                     /locus_tag="ACHKAY_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO5700016.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37442..37858)
                     /gene="flgB"
                     /locus_tag="ACHKAY_001308"
     CDS             complement(37442..37858)
                     /gene="flgB"
                     /locus_tag="ACHKAY_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO5700017.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38013..38672
                     /gene="flgA"
                     /locus_tag="ACHKAY_001309"
     CDS             38013..38672
                     /gene="flgA"
                     /locus_tag="ACHKAY_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO5700018.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38748..39041
                     /gene="flgM"
                     /locus_tag="ACHKAY_001310"
     CDS             38748..39041
                     /gene="flgM"
                     /locus_tag="ACHKAY_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO5700019.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39046..39462
                     /gene="flgN"
                     /locus_tag="ACHKAY_001311"
     CDS             39046..39462
                     /gene="flgN"
                     /locus_tag="ACHKAY_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO5700020.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39502..41037)
                     /gene="murJ"
                     /locus_tag="ACHKAY_001312"
     CDS             complement(39502..41037)
                     /gene="murJ"
                     /locus_tag="ACHKAY_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO5700021.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41147..42069)
                     /locus_tag="ACHKAY_001313"
                     /pseudo
     CDS             complement(41147..42069)
                     /locus_tag="ACHKAY_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(42071..42718)
                     /locus_tag="ACHKAY_001314"
     CDS             complement(42071..42718)
                     /locus_tag="ACHKAY_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO5700022.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42729..43313)
                     /gene="rimJ"
                     /locus_tag="ACHKAY_001315"
     CDS             complement(42729..43313)
                     /gene="rimJ"
                     /locus_tag="ACHKAY_001315"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5700023.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43549..44757
                     /gene="mdtH"
                     /locus_tag="ACHKAY_001316"
     CDS             43549..44757
                     /gene="mdtH"
                     /locus_tag="ACHKAY_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO5700024.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44821..45468
                     /gene="grxB"
                     /locus_tag="ACHKAY_001317"
     CDS             44821..45468
                     /gene="grxB"
                     /locus_tag="ACHKAY_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO5700025.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45602..46162
                     /locus_tag="ACHKAY_001318"
     CDS             45602..46162
                     /locus_tag="ACHKAY_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5700026.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46268..47314
                     /gene="pyrC"
                     /locus_tag="ACHKAY_001319"
     CDS             46268..47314
                     /gene="pyrC"
                     /locus_tag="ACHKAY_001319"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO5700027.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47388..47633
                     /gene="dinI"
                     /locus_tag="ACHKAY_001320"
     CDS             47388..47633
                     /gene="dinI"
                     /locus_tag="ACHKAY_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO5700028.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11533 a        12911 c        11863 g        11396 t
ORIGIN      
        1 gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc
       61 atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc
      121 gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg
      181 acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa
      241 tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc
      301 gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac
      361 tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg
      421 atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca
      481 cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg
      541 gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag
      601 gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactaatgca gattgccgga
      661 acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct
      721 gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca
      781 gttgcccatt actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc
      841 cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat
      901 acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct
      961 ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac
     1021 cgcgaccacc gccaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc
     1081 cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact
     1141 cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt
     1201 gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga
     1261 caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt
     1321 tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg
     1381 gaatggctgg aaatacttaa tgattcggag gagtctgaag gaatgcctga tgcgacgctt
     1441 aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg
     1501 ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta
     1561 atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc
     1621 attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg
     1681 cgcgtcaggt tacccatcag gctaccaacg gtggagaagt tcgacagcga taccagcgaa
     1741 ccatgatctt tatactgata atttttcagc ggcttaccat tcatctgcgc cagaatgttg
     1801 ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct
     1861 tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt
     1921 tgcagcgtcg gttccaccac cagctggttg atacggttag tttcaagacc accgatatct
     1981 ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat
     2041 tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc
     2101 acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat
     2161 gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg
     2221 cctttgtaac cgtagctgtg cagttgcttg accgcgttgt gcaattcagc ggagagttct
     2281 acacccgtcg cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg
     2341 gcggagtatt tcaggaagag attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg
     2401 ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta
     2461 cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct
     2521 ttctcatcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg
     2581 gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg
     2641 acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca cagatggctg
     2701 tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc
     2761 tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatctt tttcaatggc
     2821 gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata
     2881 gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg
     2941 aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg
     3001 cgactttacc gggtgagatc aaaaaacaga gtttattgtt tattatttga gcaagtccgg
     3061 aagggcgata tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat aagaggcgca
     3121 tcaggcattg atgctcgaat gtcggatgcg gcgtgaacgc cttatccgac ccacagaatc
     3181 cggagcgcat caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt
     3241 ttgaacgctt taatgcgctg taaatgcggg gagatattct tgaatttgtg cgtctgctct
     3301 tcgtcccaga caatctcgta ataatgatgc agctcttcag atgtccgctg gctgttaagc
     3361 gcttcatcat tacgcgacaa aatcaccagg caacgatcgc gattcttctc acggaagttg
     3421 gtcacacact tagtggcaat atcggcatac tcttccgggc gatcaatctt gccttccatg
     3481 ttctcataag ggaacaaatt agggttgaag atcacctggc ggatatcgca aagaaaacca
     3541 atccgttccg cccagtatcc gcccagacca acgccgcaaa tcagcggacg ctcgtcgacg
     3601 ttcagttgca acattttgtc cacttctttg agcagatgct gcatatcatg tttcggatgc
     3661 cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa actgcaattg taagactttc
     3721 tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat agataatcat cgcttcctca
     3781 cataagccag ggttagtgac ctgctttctc ttcctgccag cgttcatgta actgattcag
     3841 acgggtgctg atcgctttcc agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc
     3901 tttatgatac aaacgtgtaa cacgttctgc cttgatcggt gacagattat ctaacacgct
     3961 gacggcccct ttacgattat tgcagaccag gatcatatcg caacccgcat ccagtgatgc
     4021 ctgcccgcgt tcggcataac tgcccataat cgcggcacct tccatcgata aatcgtcaga
     4081 gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg gttttcagcc agtagggaga
     4141 accgctcgcc ggacgcggat caacatcact gtagatcaca tgcgcaggca taatggcgtc
     4201 gagtttattt tcgcggatta aggaactgaa gaccgacata tctttagcgc gaatctccgc
     4261 ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct gccgttactg caccgtgtcc
     4321 tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc ataccatcaa taaaccggct
     4381 ggcaattgcc agggcttttt gtggatcggc atgataagaa cgctcgccaa tcgccgcgct
     4441 gatatgcccg acatccagca ctggcgcaaa gctgatatca atatccatag cgatcatttc
     4501 gctggccatc agccaacccg cctcttgcgc cagtttgccg ccctcttcca ttcctgacag
     4561 cgcagcgaat gattgcgccg ctggcaagcg ggtaaaacct tcacgaaaac gctgcacgcg
     4621 tccaccttcc tgatctaccg ccaccaccag atgattgcgc gaagctgcgc ggatctggcg
     4681 caccagttca cgcaactggg caggatcatg atagttacgc gtaaagagaa tcagccctcc
     4741 caccagcgga tgcgcaagta tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc
     4801 caacattact ggacccacac tgctctcctt attgttttat taatagctgc cgccaggtgt
     4861 catcggccag cctgataaat tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc
     4921 ctgctttgag catcagcagc cagggaaacc atcgcctgac ctgacgccat aattgcgccg
     4981 gataaatctt cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgt tcagtatttt
     5041 ccacccacac tgccgccagt tccagcgcga tatcaccatc tccggcatac tcccagtcga
     5101 tgagttttaa ccctgacgcg ctatgcacta aatttccggc gtggacatcc atatgcaatg
     5161 gacttaagcg taaaggccgt ggttcccgcg ctttgcgcag acgttttaac attcgcagcc
     5221 aacccactgt ccgccgcgcc ggatcgcttt gctgccagta cagttccagt aacggcaaca
     5281 gcgttattcg ccagccaaaa cgtggttgtt gatgtagata atacagcaag cctgccagtt
     5341 cgttggtatc cggcaaatac gtttttacct cgccgggcag atagtcgact accatccagt
     5401 cacggagata taaatgcggc ttcggtgcaa tgcttgcggg tagttgtgat aaagcccgat
     5461 actggcgcaa gaacgcggac tgcggcgcat caggatcgtg cggctgacgc acaacaaaac
     5521 gctggccctg atgttcaatg agaaaactcc cgccactaag cccactatta aacgtcgtga
     5581 cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc gcgcatgatg ggattattgc
     5641 tgcgaaacgg cacctttacc tgaccagata atttcgcccg tctgcaccag catcagctgc
     5701 atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg agtacagcac gtaatgagcg
     5761 ccgacattgc gggcaatgcc tatggcttta ctacgggtac ctaaactgtc ctgcggcgac
     5821 aaacctaact gttgcttagc catcgacagc tgctgggcgg aaaccagggt aaatttcccg
     5881 ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta
     5941 gtacggttgt taacgctatc aaccagcagg acgctacccg cagtcacccc gtcagcccca
     6001 agcatcttac tgaccatcgg ctgcattgcg ccattccagt cataatggcg aatatgcggc
     6061 gcaggcggtg ccgtttgatc ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc
     6121 accgagggca ctgtggggac aggctgttgt ggctcggctg gttgttccgg cgctggtttc
     6181 acttcttcta ccggtgcagg ttcacgttgc cccacacacc cggcgagaaa catcgccagc
     6241 gcggtaatca aggcgtagcg actcattttt gtcatcaaga ttcacccctt acaaatatag
     6301 ataaagtctg actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga
     6361 atgtgcggga atggtaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc
     6421 gtcataccag taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt
     6481 tgaggatgct gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc
     6541 cgcagccaat aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg
     6601 atgtgaacga cagcccacca gcagtaacaa cgccagagac accagcccaa agcatccttt
     6661 tctcatcgtt acagaccttt atgtgccagc attggtccca gcggacggcc acccaacaag
     6721 tgcatatgga tgtggtaaac ctcttgtccg ccatggcggt tggtattcat gatcagacga
     6781 tagccatctt cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt
     6841 cccagcgcct gctcatgctc agctgacacg tcgttcacag tcggaatgag gatattcgga
     6901 atgatcagaa tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca
     6961 tcctggtaga cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct
     7021 tctgccacga cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcgcca
     7081 cactgcgagt atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt
     7141 cttaagcaaa aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg
     7201 aatgcgctga aaagacattc tctgtttggt ttacacgtca aatatggatg cgtatatttc
     7261 tcatttgcat ttacatacaa aattattcgc acactaaata taaccgctca tcaccctcct
     7321 ccggcatgag cctgacgaca acataaacca agagatttca aatgctttca acacaattta
     7381 acagggataa tcaatatcaa gccagcacca aaccatcact acttgccggt tgcatagcat
     7441 tggcactatt accttctgcc gcttttgccg ctccagccac tgaagaaacg gtgattgttg
     7501 agggttcagc cacagctcca gatgatggcg aaaatgatta cagcgtaacg tctacctctg
     7561 cgggtaccaa aatgcagatg actcaacgtg atattcctca gtcggtcact attgttagcc
     7621 agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa aacacgctgg
     7681 ggatcagcaa aagtcaggcg gattccgatc gtgctcttta ttattcccgc ggattccaga
     7741 tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg aatctgggcg
     7801 acgcactttc tgatatggca ctctttgaac gcgtagaagt agtgcgtggc gcgacaggac
     7861 tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa cacgcgacca
     7921 gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa gaacggtatg
     7981 tggcggattt acaaagccca ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg
     8041 gctaccagaa taacgactca tggctggacc gctacaacag tgaaaagacc ttcttctccg
     8101 gcattgtcga tgctgattta ggcgatctta cgacgctttc agccggttac gaatatcagc
     8161 acattgatgt taatagccca acctggggcg gtttaccgcg ctggaatact gatggcagca
     8221 gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtacaac gataaagaga
     8281 tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga tacctggcaa gcgacgctga
     8341 atgccaccca ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa
     8401 acaaagcgga tggtatgctg attgggccat acagtaatta tggacctggc tttgattatg
     8461 tcggcggcac cggttggaac agtggcaaac gtaaagttga tgcgctggat ttgttcgctg
     8521 acggtagtta tgaattgttt ggtcgtcagc acaatctaat gtttggtggc agttacagca
     8581 aacaaaacaa tcgttacttc agttcatggg ccaacatctt cccggatgaa attggcagtt
     8641 tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc ctggcgcagg
     8701 acgataccac acatattaaa tcgttatatg ctgccactcg tgtcaccctt gccgatccgc
     8761 tgcatctgat cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca
     8821 tggagaaaaa ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt
     8881 cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca
     8941 aatatctggc tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga
     9001 atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt
     9061 ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagca gtggatggga
     9121 cagtcagtaa aggggtggaa tttgaactca acggcgcaat taccgacaac tggcagctga
     9181 catttggcgc aacgcgctat attgcagagg ataacgaagg aaacgccgtt aatcctaatc
     9241 tgccacgcac cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga
     9301 cagtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg
     9361 gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc cgctaccagg
     9421 tgacgaaaaa cttctcgtta caggggaacg tcaataacct gttcgacaaa acctacgata
     9481 ccaacgtgga aggttctatc gtctacggcg caccgcgtaa tttcagcatt accggcacgt
     9541 atcaattctg aatagcacct gtaaaaaagg cagccatctg gctgccttag tctccccaac
     9601 gtcttacaga ttagtggtta cggatgtact catccatctc ggttttcagg ttgtcggatt
     9661 tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt
     9721 tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa
     9781 tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg
     9841 ccatttcgct ggtacctgtc gcttttgcat cttcagtcgc gtcttcacga cccggcgttt
     9901 tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatc gcataaccga
     9961 taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg atgaagtcga
    10021 tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg
    10081 ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaaggaga
    10141 actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag atcataatac
    10201 cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg
    10261 gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc gggtcacccg
    10321 ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa
    10381 tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt
    10441 cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga
    10501 aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga
    10561 tggcagccag gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact
    10621 caggcagctt aatacggtag aaacggttaa acatgtacgc tgcgatcgca ccggagataa
    10681 tccctccgag tacgccagta tccgccaggt gtttagaggc gatttcttca gcaggtaaat
    10741 gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg
    10801 cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa
    10861 tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg
    10921 cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggcagta
    10981 cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa
    11041 acataattga gagtgctcct gagtatgggt gcttttttta cgttctcacg cgtggcaagg
    11101 ggggagagcc tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa
    11161 ataattcagt gccaatttgt ttgacggcta tcacgtttca acttcagatc attggagaag
    11221 ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc accgcccaat
    11281 tttcggcggt gaactttact cgttttagcc attaattacg agctttaaaa aaactcattc
    11341 aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg
    11401 ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt
    11461 cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg
    11521 agtcagtttc caccagtaat cgatccaggg ggacataacg cgcagcatcg cgcagttgct
    11581 ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt
    11641 tacccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt
    11701 tttcttcgcg cagaattgcc agcgtatcgg cacgggcgtc acgggtatgg acgataaccg
    11761 gcttgttcag ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt
    11821 tagtttccgg tgtgtaataa taatccagcc cggtttcacc cagcgctaca acaccctctt
    11881 ctgccgccag acggcgtaaa tcttctacat cgtagggatc attctggttg agtggatgca
    11941 cgccacaaga aaatacgacg ttgtcacgtt cgcctaccag atcccgcata tgtaaataac
    12001 ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca
    12061 gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg
    12121 agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac aacgcccggt
    12181 tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct
    12241 gtaacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc
    12301 gagggagaaa gatggtttgc cagttcggcg accaggcccg gcacatcaac attggtcacc
    12361 tgcgcagcac catgatggcg ttttagcgca tccatcagca acgttgccag ccagtgtaaa
    12421 cgcgccggag cttgttcatg attaagggcc gctagcagcg aataccagtc gcccgatggc
    12481 acgctatatg ccaacgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac
    12541 aacgccagtg ccgcgccagg cgaaccggcg cttaagcgca atgcggcaag taatgcatcc
    12601 tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg cggcggcgca
    12661 aggtaatgta accgacaacg actacgtaat gttgccagta aacgttcagg ctcgcgggta
    12721 gccaggaaaa accaggtttc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc
    12781 gcggcgtcgg tcagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaggcgt
    12841 gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag catatttttc
    12901 cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat caactgacat
    12961 ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa ataacggctc
    13021 agggcgtaga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg
    13081 tgacctcttc cggcctgata gctggctacc agtttttcga aatcaggtcg taaccatgga
    13141 taccatctca tgcgtccaac tccttaaccc agtgggtcac ggtagtgcgg attgcatcca
    13201 tcacggcctc cagcggctgg gtggcatcaa tggtatgaat acttttatct tgtgcagcca
    13261 gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa agactcttgc tcaatacgat
    13321 ccagctcgcc gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga
    13381 gcgttaagtc ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt
    13441 ggtcaatacc acgtcctccg ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa
    13501 tcacccaggt gccgttagcc agagctggtt tgatgaccgt ttctaccagt tgaacgcgcg
    13561 cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct accgatttga
    13621 tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt tcccgagtga
    13681 aaaccatgtc gcggataccc agttgctcaa gcgtctcaac caccacatta cgcgcggtag
    13741 ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt tttttcctta
    13801 agcactttca gataatcctg cacagacttg ttatggctgg caagattggt attaaacgtg
    13861 tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca
    13921 gccttcagcg aatccgcccc cggcgtagct atcgcacccg gcggcaaacc ggtaatggta
    13981 taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc
    14041 tctcccatcc cgtaaatcac ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg
    14101 ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc tttttcgata
    14161 attgatgcca tcgtcaccag ctgattttta tctttataag gcagaccgtc cgcacgccct
    14221 tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca
    14281 tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc aatccactcc
    14341 gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc
    14401 ttgatatacg gagcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc
    14461 agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca atttcagcat ctcgcgcacg
    14521 gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata
    14581 cgcagcagcc attgaaaaac ccgtgggcga ttgatgatct tatcagcata aagctgttca
    14641 ccgagcgcca gacgtccggt ccctggcttc agggtaaata tcgtctcttc tttgataagc
    14701 aatttgctgt cggcaagatg gcgaaccttc cagacgccca caccagcggc gatacccagt
    14761 accaccagca ataacaagat aatcaataac acttttttca tgactaattc gggcgctcac
    14821 aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg ccacaggcac
    14881 gtacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct
    14941 cttccagaga ggcttgcact tcgacaagct gataagagga ttgtgccagc aaacggatac
    15001 agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacat
    15061 tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct tcgctgtcaa
    15121 ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcggatcaat acttgctcaa
    15181 gccgattaag atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta
    15241 gcgccaacgt catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa accgtaacgg
    15301 agagaatccg cgttgccggt ccactgttca atgtgctgta ccctcgcccg ccactaccgc
    15361 gactgatcac gactttcagc acaccatttt gctgttctgc tgccagcgtt ttcatctctt
    15421 gttcaagctg aggccagaag tcacagaaaa tcatcaaccg ctgacaagca tcctgtagtc
    15481 gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac
    15541 aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc ttataaccgt
    15601 taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac
    15661 ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg
    15721 gaaaatgaca acttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa
    15781 ggagttacac agcgtgtatt ccattccgct gacctgacgt gcttcatgcg gtacgaaatc
    15841 cagatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag cctgatcgcg
    15901 cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca ggtgaccggt
    15961 catagatttc gtggagctta ccaacacacg gcttgcagct tcaccgaaga tggttttcac
    16021 cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt taacgtagcc
    16081 aatctgactc gcttcaatgc ctgcatcacg cagagcattt gccattgcca gagctgcgcc
    16141 tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac
    16201 gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt caagtaccag
    16261 cataccggca ccatcgccca gtacgaagcc atcacgctct ttatcccacg ggcggctcgc
    16321 cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacgcc
    16381 cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc
    16441 gataatacgc gcagcatgac caatgttgtg cacgccggaa gtacaggcgg tcgcgataga
    16501 gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat
    16561 cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg
    16621 gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt
    16681 tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat
    16741 tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca
    16801 gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc
    16861 gattaggctg atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt
    16921 gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt
    16981 cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca ggcggtcgaa
    17041 cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa tggcagcctg
    17101 aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact cttcttccag
    17161 agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc
    17221 attgttggta acttcttcct gcttaacgcc cagctgttcg ccgataattt tcttaacgcg
    17281 ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt
    17341 cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt accacgattt
    17401 tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta attattactc
    17461 ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc agaccatgta
    17521 catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag
    17581 gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc
    17641 caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt caataaagcc
    17701 cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca gtgatttact
    17761 gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat ttcccatggt
    17821 accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca tagcgcgcat
    17881 taccgctttt gacagacgga aaacagatga aaggttggtt tcgataattt cgttccactc
    17941 ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat tgaccaggat
    18001 atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag atgccgggtc
    18061 ggtcacattc aacatcagac ctttgccgtt ggcacctaaa taatcgctga tcgcctgagc
    18121 gccattttca ctggtcgcag tgccaataac tttcgcgcca cgggctgcga gcgtttcagc
    18181 aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt
    18241 catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc
    18301 cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcacct tgcccgggcc
    18361 gacttcatag agatgttcta cgccttgcgc tgccatgtac tcaacagact tcgtccactg
    18421 aaccgggtta tacaactgac gtaccagtgc gtcacggatg gcatcaccat tggtttcgca
    18481 tttcacatca acgttattca caacagaaac tgttggtgcg ttaaaggtga ttttcgctaa
    18541 ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg gtacgctcac
    18601 tggtaacggc agcgcgcgtt ttgcgccagc agctttacag gcagcgccag cacgctcaac
    18661 cgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta ccggagaaac
    18721 gacctgacct tctgcagctt cttcacacgc tttcgcaata gacgcatcat ccagaccgat
    18781 gattgccgcc atagcgccag tgccttccgg tacggcttct tgcatgaact taccgcgcat
    18841 ctcaaccaga cgcaccgcat cagcgaaatt aatcacgcca gcgcaaacca gggcggagta
    18901 ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta ccgccctgct gctgccatac
    18961 gcgatacagc gcaacagatg caatcaacag cgctggctgg gtttgccagg ttttattcag
    19021 ttcttcagct ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc
    19081 ttcagcaaac gtttcttcga caattggata gctcgccgcc atatcagcca gcattccaac
    19141 ggtttgagaa ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc
    19201 ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt caagcagaac
    19261 caactgcccc ggcttaatgc gcccgtcgcg tacagcttca tccagcgcgc acgggacaga
    19321 ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca tagacatacc
    19381 gagttttttc gccgttgcac tgataatacg caggttagcc tgatgcggaa ccagccagtc
    19441 cagttgagaa cggtcaagat tatttgccgc cagcgtctca tcaacgatgt gcgccagttc
    19501 cgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg aattctctgg
    19561 attcacacgg tcagcattag gcagcgtcag caactcacca tagctaccgt cggcatgcag
    19621 atgggtggag atgattcccg gctcttcaga ggcagccagc accgcagcgc ccgcgccatc
    19681 gccaaaaata ataatagtcc cacgatcggt tggatcgcag gtgcgcgcca gtacatcgga
    19741 accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg ctacgcttaa
    19801 tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc
    19861 caacatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc
    19921 cacaacgatc aggccaatct ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc
    19981 cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat
    20041 accggtacga gtgacaatcc actcgtcaga ggtgtccacc attttttcca aatcggcgtt
    20101 tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat acatgtacgc
    20161 tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg gcagcgtcct
    20221 gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagct gggtatacag
    20281 attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt
    20341 caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc accgtgccgc
    20401 gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg aatcgcctcg
    20461 tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt tttttccctt
    20521 caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc gtctttaacg
    20581 tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt aacaactcat
    20641 tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc gctgaggcat
    20701 cccgaatact gtcgagaccc tttacttctt cttcaccaat attgagcaac gccacgcgag
    20761 gattgggaat ttccaccacc tcttcagcca gaactgagcc cataatggca aattgcacca
    20821 acattgtgct gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt ttgccctttt
    20881 gctgatgtgg taataccgtc accagcgccg gacgctcaat cccctccagg ggcttgagta
    20941 ataattttgc cagccccatc agtgccccgg tattaccggc actgacacag gcttgcgctc
    21001 gaccttcttt caccagctcc agggccatac gcattgaact cccacgactg gcgcggatag
    21061 cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc tgcagacgcg
    21121 aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga ttgccgacta
    21181 aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg
    21241 aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc aaggtatcgt
    21301 cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa gcttcatcac
    21361 gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat
    21421 gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg tgactgcggt
    21481 cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat tctgttgtac
    21541 ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga gcaccaatta
    21601 cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt cttcaggcag
    21661 ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat gcaccggaac
    21721 taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga tttcaccgaa
    21781 ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt cgtctgaacg
    21841 cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg
    21901 acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac gttggttatc
    21961 gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg attcggcgac
    22021 gcgctcaacc tgatcagggg tatagatacc ctggtaatca aggcgttttt gagccgtacg
    22081 aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat attaactttg
    22141 taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa taattcgcac
    22201 acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca acctgcaaaa
    22261 cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga aaaaaatatg
    22321 cctaaactta ttttagcctc cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa
    22381 atctcttttg aatgtgcagc accagaggtc gacgaaaccc cacgcagcga cgaatcaccg
    22441 cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg
    22501 gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat caccggtaaa
    22561 ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc
    22621 ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctacaaac agaagtggag
    22681 ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta cgtgcgtaag
    22741 gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat tacgctgttt
    22801 gagcgtttag agggacgtga ccctaacacg ctggttggtt taccgctaat cgcgctgtgt
    22861 cagatgttac gtcgggaagg gaaaaacccg ctgatgggat agttgtttgc gccggatgcg
    22921 gttacgtcgc atccggcaat cggtgcaggc ggcctgatgc ggcgtaaacg ccttatcagg
    22981 ccaaccgccc attatcaggc ttatatgctt gtattagcgc gcgttacgca gcttttgcaa
    23041 acaacgcttc aaaccttcat ccatcggcgc ttcgatacgc atcacctcac cggtccccgg
    23101 atgggtaaac ttcaacgctg cggcgtgcag gaacagacga tttaatcccg tgcctgcttc
    23161 agtgagctgt ctgtcaaatt cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc
    23221 cgcatactgt gtatgcacac ggatctgatg agtacgtccg gttaccgggc tacaacgcac
    23281 cagggtggca aatgcatagc gttcttccac tttaaagcgt gtttccgacg gtttgccttc
    23341 ctgactgaca cgcacgatac gttcgccgct ttgcagaata tttttcaata acggcgcttg
    23401 aacgctcttc acatgcgact gccactgacc gcgcaccagc gccaggtaat ctttttgcat
    23461 tcctttttca cgtaattgct catgcagaga acgcaacgcc gagcgttttt tcgctaccag
    23521 caacacacct gaagtgtccc ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg
    23581 gcgcaacgcc cgcaaacctt cgataacgcc gaagcttaaa ccactgccgc catgtaccgc
    23641 cgtaccggaa ggtttattca gcaccaggat atgatcatct tcatataaga tgacgtccgc
    23701 cagcgccgcc accttttgca gatgtggcga aaccgcctct tcttcccgct cagcaacgcg
    23761 aaccggtggg atacgcacct catcacccgc ttcgagttta tattcaggct taatacgttt
    23821 tttgttcacc cgcacttcac ctttacgcaa aatacggtaa atcatacttt ttggtacgcc
    23881 tttcaattgg gtacgcaaaa agttatcgat acgttgcccc gcttcgtcag cggtgatagc
    23941 aacaattttt acggatggag tctctgtttt catggtgggc gattctaatt agccaacagg
    24001 attcgcgcca ctcatttttc tatgcttata tttagtttta caccttatta cttcactacg
    24061 tgatcacttt attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt
    24121 aagcgggtga taaatggtaa aagtcatctt gctataacaa ggcttgcagt ggaataatga
    24181 ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt acggaataac ccattttgcc
    24241 cgaccgatca tccacgcagc aatggcgtaa gacgtattga tctttcaggc agttagcggg
    24301 ctgcgggttg cagtccttac cggtagatgg aaatatttct ggagagtaat acccagtctg
    24361 tttctttgat aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct
    24421 ctttgagctg acgataaccg tgaggttggc gacgcgacta gacacgaggc catcggttca
    24481 cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag
    24541 ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga agagttgcgc gttgcccttg
    24601 tagatgggca gcgtctgtat gacctggata tcgaaagtcc agggcacgag cagaaaaagg
    24661 caaacatcta caaaggtaaa atcacccgca ttgaaccgag tctggaagct gcttttgttg
    24721 attacggcgc tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc gaatatttcc
    24781 ctgctaacta cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg
    24841 aagtcattgt tcagatcgat aaagaagagc gcggcaacaa aggcgcggca ttaaccacct
    24901 ttatcagtct ggcgggtagc tatctggttc tgatgccgaa caacccgcgc gcgggtggca
    24961 tttctcgccg tatcgaaggc gacgaccgta ccgaattaaa agaagcactg gcgagccttg
    25021 aactgccgga aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg
    25081 cgctgcaatg ggatttaagc ttccgtctga aacactggga agccatcaaa aaagccgctg
    25141 aaagccgccc ggctccgttc ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc
    25201 gcgattactt acgtcaggac atcggcgaaa tccttatcga taacccgaaa gtgctcgaac
    25261 tggcacgtca gcatatcgct gcattaggtc gcccggattt cagcagcaaa atcaaactgt
    25321 acaccggcga gatcccgctg ttcagccact accagatcga gtcgcagatc gaatccgcct
    25381 tccagcgtga agtccgtctg ccgtccggtg gttccattgt tatcgacagc accgaagcgt
    25441 taaccgccat cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg
    25501 cgtttaacac caacctcgaa gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc
    25561 tcggcggcct gattgttatc gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg
    25621 tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata
    25681 tttctcgctt tggcctgctg gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat
    25741 ccagtcatca cgtttgtccg cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc
    25801 tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct gaaagagaac acccaggaag
    25861 ttcacgccat tgttcctgtg ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg
    25921 taaatgccat tgaaactcgt caggacggtg tgcgctgtgt aattgtgcca aacgatcaga
    25981 tggaaacccc gcactaccac gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa
    26041 gctacatgct gccgaagctg catgaagaag cgatggcgct gccgtctgaa gaagagttcg
    26101 ctgaacgtaa gcgtccggaa caacctgcgc tggcaacctt tgccatgccg gatgtgccgc
    26161 cagcgccaac gccagctgaa cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac
    26221 cggcaacacc agcaactcct gcacaacctg ggctgttgag ccgcttcttc ggcgcactga
    26281 aagcgctgtt cagcggtagt gaagaaacca aaccgaccga gcaaccagca ccgaaagcag
    26341 aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc
    26401 gtaatgagcg ccgcgacacc cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa
    26461 accgtcgtaa tcgtcgccag gcacagcagc agactgccga gacgcgtgag agccgtcagc
    26521 aagctgaggt aacggaaaaa gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac
    26581 gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg
    26641 ttgaagagca atctgttcag gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc
    26701 gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta gccgaagaag
    26761 cggtagtcgc accggtggtt gaagaaactg ccgctgccga accaattgtt caggaagctc
    26821 cagctccacg cacagaactg gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag
    26881 aacagcaaga agagaacaat gctgataacc gtgacaacgg tggcatgccg cgtcgttctc
    26941 gccgctcgcc tcgtcacctg cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc
    27001 gttatccaac ccagtcgcca atgccgttga ccgtagcgtg cgcgtctccg gaactggcct
    27061 ctggcaaagt ctggatccgc tatccaattg tacgtccgca agatgtacaa gttgaagagc
    27121 agcgcgaaca ggaagaagta caagtgcagc cgatggtgac tgaggtccct gtcgccgccg
    27181 ctgtcgaacc tgttgttagc gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc
    27241 ccgtaccggt tgccgaaccg caaccggaag tggttgaaac gacgcatcct gaagtgattg
    27301 ccgccgcggt aactgaacag ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg
    27361 aagtcgcaga acacgcagaa ccggtggttg aaccgcagga agagacggca gatattgaag
    27421 aagttgccga aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg
    27481 cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt agccgcggtt gaaccagaga
    27541 tcaccgttga gcataaccac gctaccgcgc caatgacgcg cgctccggca ccggaatatg
    27601 ttccggaggc accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag
    27661 gtgccgcagg tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac
    27721 ctgttgagta ataattagct caaagtaatc aagccctggt aactgccagg gcttttttat
    27781 ttcatctttg aatcaacata ttaaaattca tcaaatggca tccttgctaa ccaacaatgc
    27841 aaaataggca aaaaaatgcc cgccccggcg atcccagagc aggcagacaa aacataccca
    27901 gtttcatgat atgttttaaa gcgagcttat ttgttgagct ggaagagcga caatccctgc
    27961 atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa
    28021 atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg
    28081 cgatcgctac ctaatgaatc cagcgactcc agttcgttca gctgcgtgcc taattccgcg
    28141 cgaactgtaa ggacattatt cagcgagttt ttcagtccac ggttggtttt atccaatgcc
    28201 gcagcggcga tttctttatc cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg
    28261 gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg ttccgctacc
    28321 gcgttactgg taatactgtc gaaaattttg tcgcccgtat gtcctatcac catcgaacgc
    28381 gaagcatcga cctgttgcct aatactttct gctccaccga cgtatttccc tttctcttcg
    28441 ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt accgtcagtg
    28501 gtgttcgcca gattcagcaa ctggtcacga agcccctgga tatccgtagc cagcgaggcc
    28561 cggtcatcgt cactcaaagt gccattgctg gcgtagacaa ttttttcctg agcattctgg
    28621 atagcagtgg tgacctggct aagtacactc tcttccagtg acactttttg agtggcgaaa
    28681 gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac aaccgcttgt
    28741 gatgctgcaa tgggatcgtc agaagggtta acgactcgct tacctgtcga catctgctcg
    28801 ccgtacttca tccattccgc ctgagaattg gtgatgccac gcatgttttg ctggtacatc
    28861 atctgggtac tgaaacgcat cttatctccc cttagcgaat gttaatcagc gcatcaaaaa
    28921 tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga aaacgttgca
    28981 gatttccgta ctcctcatcg agattgacac cggaaatcga ctgctgctga ttggaaagct
    29041 gcgtcaccac attaccttgc gtggcgctac tggttttcaa cgtcgcggtt ttattaccga
    29101 tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc accgttttac
    29161 tgttgctttg cagatccagc agggcctgac cgttgcggtt atcgctatca cccgcatctt
    29221 cttcgctcgc catcgctatt ttcgcttcgt cggtgattaa tacatccatg ttgacgatag
    29281 cgtcacttac tggtttcagc gtgaagctgt cgttaacagc aggcgttcct gtaaacgtca
    29341 actccagacc atcaaatgcc actttaccgt tggcatccgg cgtcaccgta aaagtggtat
    29401 tactggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg taatcagtcg
    29461 ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc aacgtcacct gtgtttttcg
    29521 tgttttgcag aaccgcgggc ttaccgatag caaagaaatc ttcaccagca tcgccgttgg
    29581 catcaaatcc ggctttgtgt tggctgttga aagcctcggc aaatgccagc gccagttgtc
    29641 caagcgtatt acgcgtctgg tccagatcct gagaacggaa tgtcagaatg ccgcccagcg
    29701 acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca tcaacgtaag
    29761 cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc cgccagttgc cgcgccgtac
    29821 ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt ataagtaccg ccatcctgaa
    29881 cgctgacttc tacgccaaca atctggttta attcgctcac cagttgatcg cgttgatcca
    29941 gcagattgtt aggtgacgcc cctgccccca cgcctgtcag gcgcgagatt tgatcgttca
    30001 ggctggcaat ttgtttagcg taattgttga tctgatcaac gctggcacct atcgcgatat
    30061 tgacctgttt gtcctggtcg cgcagatatt gatcggtggt tttaaactga ttcaccaacc
    30121 cttctgattt cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta ctcaccagcg
    30181 tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta ctggtggaga
    30241 gcatattgtc gattttcgac atctgctcat agcgggcagt caggccgcta ctttgcgtct
    30301 gcgccgcacg taactggttg gtaataaaag catcatactc acgctgcaca ccagaaacgt
    30361 agacaccatt gccaacccag ccgccagcgc ccaacgtgct attggcctgc gccataatag
    30421 tggtttggcg ggtatatccg gcaacgttat agctggagat attattactt gccgtattta
    30481 acgccgcctg ggccgcgttc agtccgctca tggcgttatt gatcaagctg gacatggagg
    30541 ttccttataa gccttcaatt acagagtatt atcggcagcg acccgccgga cttgagttat
    30601 tcagaacaga ttatcaatgt tcatgctgta ggttttgctc accttgtcgc ttatcgattt
    30661 catctgctgg atcatgttgg tgagtttacg ggcatagtga ggatcggtag cataccccgc
    30721 gtcctgtagg gcctgcgccc cctgctccgc actcgcggcg gtcgtcacgg cggcgtagcg
    30781 cgggttacgt gttaacagcc caacgtaatc cgacaatgct tccagatacg agctgtagac
    30841 ccgaaacttc gcttttactt tcttcgcttc gccgttttca tattcagtcg tggtgatttc
    30901 agtaactggc cctttccagt tgccagaggc tttgacacca aacaggttat agctcggctc
    30961 gccgttttcg cggcggattt gccgttgccc ccaaccagat tccagtgccg cctgagcgag
    31021 gatcaaatga tgtggcacac cgctttgctg gcttgccagt tgggcgggca gcgagagttg
    31081 cgcgaggaat gctttactgt cacccggcag cgaatcatcg tagttacgtg gcacggcctt
    31141 ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc acagtttcga gcgggaattt
    31201 catcggtgct gctggcgtgg actcctctgg caatggttgt tctggcgtca tctgtttaac
    31261 catcatctct gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa tctgctggtc
    31321 atacatactg gtatacaggc gagtgtgctc gctgctgaac aggccatctt ttggtaaagc
    31381 gtcgcgcatg cttttcaaca tcatctgcac gaacatccct tccacctgac gggccaccgg
    31441 acggatattt gccgccggat cttcgcccgc tttcgccttt agttcgttga gtgattgcgc
    31501 atcccaggcc gcacttgcca gtagtttgct gtcgctgatc atcagatgat ttccagtttt
    31561 gcccgcagac atcccgcact ttgcattgat tgcagtatgg acatcagatc catcggcgta
    31621 gcgcccagcg cattgagcgc gcgcaccacg ttattgaggc tggcgctgga acgtacgctt
    31681 tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac cacagtctgt
    31741 ccaccaccaa acggtgtatc tggctggctg acattggcct gacgattgac tgttactgag
    31801 agatttccct gcgctaccgc gcagctgtcg agggtcactt cgcgattcat caccaccgaa
    31861 ccggtgcgcg agttaatcac tactttagcg tcctgcgggg tgacattaac ctgcatattc
    31921 tggatatctg caaggaaacg gacctgggaa ctgttgccac ttggtacgcg cacctgaata
    31981 gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca cgcggttgat ggtgtcagcg
    32041 atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca aattaagggt attcccgacg
    32101 ccaaactggc tgggcaattc acgttcaata accgcaccat tggtgatccg tccaccgttc
    32161 agttggttca cctgaacact gctaccgcca gcggaggctc ctgcgccgcc aaccagaata
    32221 ttgccctgcg ccagcgcata cacctgactg tcaacgccct taagcggcgt catcaacaac
    32281 gtacctccac gcaagctttt ggcatttccc atggaagaaa ccaccacatc gatggtttgc
    32341 ccctgacgtc caaacggagg aagtgacgct gtcaccatta ccgcagcgac gttttttagc
    32401 tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt attaagcgtt
    32461 tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc catccagccc caccaccaga
    32521 ccatagccaa tcagtgagtt ttgccttacc ccctgaacac tggtgagatc gcgaatacgc
    32581 tcagcctgag ccgccgtcgt aaccagtaga agaattaatg cagagagaaa tttaatcacc
    32641 acagcctcac ttacattggc gacaggttaa ggaagaaacg ctgcaaccag cccatatttt
    32701 gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg cgcatccgcc acctgagtag
    32761 acggtacggt attgctgccg ctgatagtgc gtggattaac cacgccagag aagcgaataa
    32821 attcggtacc ctgattaatg gcaatctgtt tttcacccac cacatgcagg ttgccgttga
    32881 ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg gcattggccc
    32941 cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac atcggcacga gcgttaccaa
    33001 acagcccctg caaatagcgc ggcacagtat caaagccaaa attagtttta ccgtcacggc
    33061 tggcattcgc agaggagctt ttgctggcgc tgacgttctc ctgcaacacg atggtcagcg
    33121 tatcgccaat attgcgtggt cgacgatctt caaacagcgg ttgatagcca tagttaatcg
    33181 gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt cggcccggga accggttgtg
    33241 cactggtcgc cccctgcacc agcggcgtgg agggtatcca ggcgcagccg gttagtgaaa
    33301 gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt tttttgcatg gctatcatct
    33361 tcaaaaatca gtaaaccggt gaacctgcca ccggttaagc cttagagttg cgtcagtttt
    33421 tgcagcatct gatcggtggt ggacaccgct ttactgttga tttcgtaagc gcgttgcacc
    33481 tgaatcatat tgaccagttc ttccgccacg ttgacgttag acgtttcaac atacccttga
    33541 tacagcagtc ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc agaggattgc
    33601 gtttcggtgt agaggttttc gccaatgctc tccagcccgg tgtcattcat aaaggtggtg
    33661 agattgagct gcccaacctg aaccggagct gcctggcctt gttgggttac gctgaccacg
    33721 ccatcacgac cgatggtgat acttaacgca ttcgccggaa tggtgatcgc tggctgcacc
    33781 tgaaaaccac cagccgtcac cagctgcccg ttctgatcca cctggaaaga gccgtcacgg
    33841 gtataggctg atgaaccatc tggcaacatc acctggaaaa agccctgccc tttaatcgcg
    33901 acatctttgc tgttgttggt ctgcgacagg tttccctggc tgtgtaagcg ttcagtggcg
    33961 accgggcgta cccccgtgcc gatttgtaat ccggagggta aggtggtttg ttcggaagac
    34021 tgtgcccccg gctggcgaat ggtttgataa agcagatctt caaacaccgc gcgctgacgc
    34081 ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa tgacgtccat attggtttgc
    34141 tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga tcataaaatg tcctgtagtg
    34201 gattagtgac aagcagagca gaagcacgcg acagtgtgaa tttcccctca ccctaaccct
    34261 ctccccaaag gggcgaggga atagtccgcg cgcttttatt gcaatgtcat caatcgttca
    34321 attaactcat cgacagcagt tggttggcac ggcctgcgtt atcatcgacg ctgctgatca
    34381 ccttcatctg catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg
    34441 cattgacgtt actgccttcc agaacccccg acatcacacg caaggttgga tctgcctgca
    34501 gtatcggccc acgcgtggcc tggctttctg cgcttaaacg aaaaatgccg tcgtcaccgc
    34561 gttgcacttc gctgcccgtg gctttcacca gtttcagacg ccccactggc gcaaccgtat
    34621 ttgccggatc gcccggattg agcgccgaga ttgtgccatc ggcagcaata gtgatttccg
    34681 ccccttccgg cacagcaatt ggcccagcct cgcctatcac cggatgcccc tgaattgtca
    34741 gttgcccggt gggatcaacc tgaatgctgc cattacgcgt atacccttcg ctgccgtcag
    34801 cggactgcac ggccagccag ccatcctgct gcaatgcaac gtccagcggg cgcgaggtgt
    34861 aatccatctt gccgggcgtc atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg
    34921 tgggcagtga aagcccttcc accggcactg cacgcaaggc gtttaactgc gcgcgaaaac
    34981 cgggcgtaga ggcattggcc agattactgg cggttaccgc ctgttgattc agcgtctggc
    35041 tggctgctcc catcgcggta taaattgcgt gatccattga gctatcccgt cagcgattag
    35101 cgtaagttaa ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg ggcgttagac
    35161 tgatagttac gctgggcaac gatcatattg accagttctt tactgagatc gacgttggac
    35221 gcttccaacg caccgttggt cagggtgcca aagttgcccg tcccggctgt ccccaacagc
    35281 gccacgccag aagattgcgt cgcagaccag acgttgtcgc cttcggatgc cagaccttcg
    35341 ttgttggcaa agttcgccag tacaatctgc cccagcagtt gtgtttgttc gttggaatag
    35401 ttgccgacaa ccgtaccgtc atcattgatt tgataactca ccagatcgcc cggtttgtag
    35461 ccattctggg tggttgccac aatattgtta gcgccggtat tttgctgcat ggagttgagg
    35521 aagctcaggc taaacgtggc gggatctgcg ccgttaattg cgccagtggt aatattctct
    35581 gttggatttg aggtcagaac gccattggca ttaaacacca gcttcattgc aggctcggct
    35641 gtacctgttg gatcactgct atcctgggtg tagacctgcc agttattatc cccggtcttc
    35701 acaaagtaga cgctcatgtc atgagcatta ccctgactgt cgaaaacagt caccgaacct
    35761 tttttgttat agctatccgc attgctggcg ctgaatggcg taacagtagg aagcggatca
    35821 ctggaattca ggttgatctg catcgatgcc gtggtggtag ttttcgctgc catcagggta
    35881 ttcgggatcg aaatattggt cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt
    35941 gccgggtaac ccgtcagctg taaaccttgc atattcacca ggttacggtt ttcatccagc
    36001 ttaaattgtc cgttacggct gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa
    36061 ccgttctggc tgatagcaac gtccagacct cgcccggtgt tggtggtcgt gccatcggta
    36121 aagtcctgag tgataccggc aacttttacc cccagtccca ctttcgaacc ggcaaacata
    36181 tcggcaaaag aggccgtgcc tgatttaaag ccgtaggtgg cggagttggc gatattgttg
    36241 ccaataacat cgaggttggt ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc
    36301 atgactgact cctgaaatgt gaaggcttaa attatctgcc gtacttcgtc gagggtggtg
    36361 gtgccgtaag tgccgagatc cagcgtatta ccgctgttgc cgcggatcac accctgcacc
    36421 agagcaaact gcagcggctg ggcaaccagt tgtgtaccac cgttactggc gctaatcgct
    36481 acattgtaag aaccgttcgg cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa
    36541 ctgtgaactc cggcggtcag ttcaccaata tcaatggtgc gcacaaccgc accattttta
    36601 tcggtgatgg tggccgtaac tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg
    36661 gtcgtggtca cagctccttc ttcactgccg gttcccgcaa gaacagtggt gccggggatc
    36721 atcacgccgt gaccgatcag gttactggcc tgtaacgact ggctgttatc aatctgtccg
    36781 gaaatagatc cgagtgtggt attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc
    36841 aattgcgacg tcagctcgtt gttttccatt ggattggtcg ggtcctggtt tttcagctgc
    36901 gccaccagca aagtcagaaa actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa
    36961 ctactgctgg tggtactgac gccggtattt gtcggatcgg tggtggttac cgcaatggac
    37021 atagctttct cctttattga ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt
    37081 tgagcacttc aacgttggcc tgatagctgc gtgacgccga catggtgtta accatctccc
    37141 cgacgacatc aacgttcggc atttttacgt agccctttgc atctgccagc ggattacccg
    37201 gttcataaac cagtttgtcc ggggcctgac tttctataac atcggcaacc tttacgccgc
    37261 ctgtcgcagc acctggtgca gcgttaacct ggaataccac ctgttttgcc cgatatggct
    37321 gtccatcagg accggtcacg ctatcagcat ttgccagatt actggccgcc acgttcaggc
    37381 gctgggactg ggcagttaac gccgacccgg cgatatcaaa aatattcagc agtgccatcc
    37441 gttaatttcc gctctgtaaa acgttcatca tgcctttgat ttgcccgctc aacgcgctaa
    37501 ggctcatctg gtattgcagg ctgttatcgg caaactgggt gcgttcgcga tccatatcga
    37561 cggtattacc gtcaagcgaa ggctggtccg gaatacggta ttgcagttct gcggtaggag
    37621 gcgtcagcgc ctgcgccggg atgtgttgcg ttgaggtcat cgtcagtgca accacactgg
    37681 ttgcatcccg tccacgttgc atgacttttt taagttcact ggcaaaatcg atatcgcgcg
    37741 cctgataacc aggggtatcg gcattggcga tgtttgctgc cagcacttcc tgacgctggg
    37801 cgcgcagatt gagcgcctct tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat
    37861 ctcctccgca ggtatcaaaa ttctgccatc agcttaaatg cctttacgcg cgcgttatcg
    37921 gcggaataaa cgcaaaatgg gtcgctattt atgccgttga tggtcattgc ggacaggtac
    37981 aattcacgtt gtagaaatgg ctgggggcga aaatgctgac aataaaacgt agcgtggcga
    38041 tcatcgcgat actgttcagt ccgttaagta cggcgagcaa tctcacatcg caattgcaca
    38101 acttttttag cgcccaactc gcgggggtaa gtgatgaggt tcgtgtttct attcgtacag
    38161 cgcccaatct actaccgcca tgcgagcagc cattgctttc gatgagcaat aattcccgcc
    38221 tgtggggcaa tgtgaatgtg ttggcacgct gcggtaacga caaacgatat ttacaggtta
    38281 atgtgcaggc cacaggaaat tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc
    38341 tggaagctgg caatgtcaaa ctaaaacgcg gacggctgga taccctgcca ccgcgtacgg
    38401 tgctggatat caatcaactt gttgatgcca ttagcctgcg cgatctatca cccgatcaac
    38461 ctatccagtt aacccagttt cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg
    38521 tgatcgccag cggtgatggg tttagcgcca acgcagaagg tcaggcgctg aacaatgcag
    38581 ccgtcgcaca gaatgcgcgg gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg
    38641 atgcagatgg gaatattctt ataaacctgt aagctgttaa agattacccg tcccttgccg
    38701 ataaataagc aacacatgat aaaagcgccc tcaatgagga ataaaccatg agtattgatc
    38761 gcacttcgcc tctgaagcct gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg
    38821 taacgaacag ccgggcggca aaaacaaccg cctccaccag caccagtgtg acgttaagcg
    38881 acgcgcaagc aaaactgatg caacccggca gcagtgatat caatcttgaa cgtgtcgaag
    38941 cgttaaaact ggcgattcgt aacggtgaac taaaaatgga caccggcaaa attgccgatg
    39001 cgctgatcaa cgaagcgcag caagacttgc agagtaactg atcgtatgac acgtcttgca
    39061 gagatcctcg accagatgtc cgctgtgctt aacgatctca aaacggtaat ggatcaagag
    39121 cagcaacatc tctctatggg gcagatcaac ggcagccagt tgcaatggat tacagaacaa
    39181 aaaagctcac tgctggcgac gctggattac ctcgaacagt tacgcaagaa agaacccaat
    39241 acagcaaata gcgttgatat tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa
    39301 ctacgccaaa tgaatcaaca taacggctgg ttactggaag gacagattga gcgcaatcaa
    39361 caggcgctgg aaatgttgaa accgcatcag gaaccgacgc tatatggggc gaacggtcag
    39421 acctcaacaa cccatcgcgg cggtaaaaag atttcgatct gaatattatc tccggcctgc
    39481 actgcaggcc ggaatgcatt gttacaccgt ccggcgggca aattctttaa ctttgaagcc
    39541 cagtaccgcc agtgcagcga agtacgcggc aatccccgcc agcacgaccg ccattaaacg
    39601 cagtaagcgc cagggcatgg tacccaatga ccactccggc atgatatgta acatacctaa
    39661 aagcacgcca gacatcacca gtaccgccac caccagacgc aacagaaacg ccatccagcc
    39721 gggttgcgga gtaaagattt tctgcttacg caactgccag taaagcagcg aagcattcag
    39781 acacgccgcc agaccaatag aaagtgacag cccggcatgt ttcaacggac caataaacgc
    39841 caggttcatc aattgcgtta aaatcagcgt aacgatggca attttcactg gcgttttaat
    39901 gtcctggcgg gaataaaagc caggagccaa cactttcact acaatcaggc caatcaaacc
    39961 caccgagtag gcaattaacg cccgctgggt catcagcgca tcaaacgcgg taaatttacc
    40021 gtactggaac agcgaaacgg ttaacggacc agaaagaatg cccaacgcaa ccgcactcgg
    40081 cagcgccaac aggaaacaaa gacgcaaccc ccagtccatc aaacggttgt attcatcatg
    40141 attgccactg gcaaaacttt tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc
    40201 cagcacaccg gacggaaact ccattaagcg gtcggcgtaa tacatccaag acaccgaacc
    40261 ggaagcaaga aacgaggcaa aaatggtgtt gatgattaag gagatctggc tcacagagac
    40321 gccaaggatc gccggtccca tctgtttcac cacgcgcatt gttccggcat cgtggaagtt
    40381 aatgcgcggc aggaccagca tgccgatctt cttcaggtgc ggtagctgat acaccagctg
    40441 caggatgccg ccgaccgtaa cagcccacgc cagcgccagc accggtgggt taaagtacgg
    40501 tgcggcaaac agcgcgaaac caatcatgct gatgttaagc agtgttggag caaacgccgg
    40561 aatcgagaag cggttccacg tattcagaat cgctcccacc agcgacgcca gggagatcag
    40621 caagatatag ggaaaggtaa tctttagtag ctggctggtc agggcaaatt tgtcagctgt
    40681 gtcagcgaag cctggcgcgg tcaccatgat cacccacggt gcggcgagca tgccagcgac
    40741 cgtcacaacc gccagcgcga gtgtcagcag gccagaaaca taagagacaa agacccgcgt
    40801 ggcgtcttca ccctgcttac ttttatattc cgccagaatc ggtacaaatg cctgggaaaa
    40861 tgccccttcg gcaaagatac ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc
    40921 gtcggttgcc atccctgcgc caaagattct ggcgacaatt gcgtctcgtg cgaagccaag
    40981 cacacgcgaa aacatggtca tcgagctgac ggcggccagc gattttaata aattcatcgg
    41041 tgttctaatc cagacccacg ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata
    41101 gtctacctgg attatggtga attgctaccg ccagatgtta cagggtttat tcactcatcg
    41161 catcgcgcca gatcttgtca acgatacgtt gcgccagcac ggcctgttcg cggcggtttg
    41221 cggaactgtc tgattttgca cacattcaat gaagtgccgc gcacagccga caaatccacg
    41281 ttgttcaaga gtgctctgcc aaccaggaat cggtttatgc actacgccct gcccgcgctc
    41341 ctcacgccat tcgcgcatat ccgtaatgtc gatgagcgca ccgtcagtca cggcctgcac
    41401 ggtttcacgc tgacttccgg cacggcgatg catacaggtg gtgatctgca aaggaccggc
    41461 cgaaaaatgg tgctcggcaa acagcatttc gccagcgtcg ttagtcagta gcgtaccgcc
    41521 atccagagag gctttgccgc ccgacaacca cagcacggta tccaccacat gcagataatc
    41581 atccagcaac gtgaaataaa gatcgtgtgg cccgacacta ttgctacgat gtttatccat
    41641 tcttagcgaa gctgcggtag cgagttgcgt ttttaactca ccgtagagtg gtgcgaaacg
    41701 acggttaaaa ccgaccatca acgtcagttt tttacgcgcc gccagttcca ccagccgttc
    41761 agcatcgcgc agattttctg ccagcggttt atcgacacag acatgtacac ccgcattgag
    41821 taacgtactg accacgtcaa agtggctggc ggtgctggaa tgcacaaaaa ccgcatcgca
    41881 actggcggca aggctggata acgaatcggc ataaggaatg cgccagcttt cacaaattgg
    41941 cagggctttc gcgcgcgtag gcgaccaggc tccttgtaac gtccagtcag acgctgccgc
    42001 cagcaccggt aaccacgctt tttgcgcaat gccacctaat cccactacgc cgatacgtaa
    42061 ttttttcact ttaatctccc agatgggcca gcaacgaatc aagacgctgt ttcagttctg
    42121 ccacttcgat ttccagggct tcgacgcggg cctgtaaatc accatcaacc gcgttcgaca
    42181 tatccgtcac cgccggctga tcttcaacct caccactgaa aagatgcatg tagcggcttt
    42241 cgcgtttacc cggttcgcgg gccagacgca ccacaaaagg accatcttcg cgatttgcca
    42301 gttgttccag cgtcgactcc acttccgcca tatcgctgaa ttcatacatt cgcgcggcgc
    42361 ggctgcgcag ttcacctggc gtctgggcac cacgcaataa caacgtggtg attaacgcca
    42421 cttccgctgc gctcagtttc agatcgccaa attctgaatt acaaaaacgt tgctcatatt
    42481 tggtgacccg attaccaaaa ccgctcactg tgcgtagata atgacgtttg accagattat
    42541 ccagctgttc ctgcacttcg gattcactca gattcatgac cggttcacgg ttcgttttct
    42601 gattacaggc cgtgactaca ccattgactg agagcgggta ttgctccggc gtggtcacct
    42661 gcttttccag caagcaacca atcacgcgag cttcaagggc agtaagttga tatttcatga
    42721 gattctcctt agcggccggg cgtccagtct ggggtagtta atgccgtcag tacgtgatcg
    42781 cgccattgtc catcaatcaa cagatagtct ttcgcatagc cttctttttc aaaacccagt
    42841 cgcgccagta aatcaccgct gcgtttattg tgcggcatat aattagccat aatgcgatga
    42901 atatgttggg tgcgctgcat ataacgaatg gctgcggtca gggcttcaaa catgagtcct
    42961 ttgccctgcc atttttgccc aatcgaataa ccgagatagc aggcatgaaa agagccacga
    43021 acaacattgg aaaaattggc aacgccaata atctcttttt cgtccgggtc gaataagcca
    43081 aagtagaaag ctgaaccttg tttatgaaat tcgttaatca tccccagcct ggcctgccag
    43141 cctgatggat aacagtggct ttcgtcgcgc actggctccc agggcttgag gaaatggcga
    43201 ttctctgcgt aataatccgc aagacgccag gcatcacgat catgcaccag acgcacgacc
    43261 agtcggtctg tggttaagcg cacttttggc acgttactgc gatagccaaa cattctatac
    43321 ctactccttc ccgtaacgtc tttaatacgc tgtaagtatt actatacctg cgcaaccaga
    43381 ccctgtgaaa agtccaaaat gccattttct gcattattca catatttgat aaaaactctc
    43441 tatcaaagcg acgttttgat aaaaaaatat tgtccccgaa gtgatgggaa aagtcttagc
    43501 ggcaatgcag aatagaaagt gctcatcttt tatttcccgg gaggggaaat gtcccgcgta
    43561 tcgcaggcga ggaacctggg taaatatttc ctgctcatcg ataatatgct ggtcgtgctg
    43621 gggttctttg ttgtcttccc gctgatctct atccgcttcg ttgatcaaat gggctgggcc
    43681 gccgtcatgg tcggtattgc tctcggtcta cgccagttta ttcagcaagg tctgggtatt
    43741 ttcggcggcg caattgccga ccgctttggt gccaaaccga tgattgttac cggtatgctg
    43801 atgcgcgccg ccggattcgc cacaatgggt atcgcccacg aaccgtggct attgtggttt
    43861 tcatgcctgc tctcgggact cggcggcacg ttgtttgatc cgccgcgttc ggcgctggtg
    43921 gtgaaactaa tccgtccaca acaacgtggt cgttttttct cgctgttgat gatgcaggac
    43981 agtgccggtg cggtcattgg cgcattgttg gggagctggc tgttgcaata cgactttcgc
    44041 ctggtctgcg ccacaggggc cgttttgttt gtgctgtgtg cggcgttcaa tgcgtggttg
    44101 ttaccggcat ggaaactctc caccgtacgc acgcccgttc gcgaaggcat gacccgcgtg
    44161 atgcgtgaca agcgttttgt cacctatgtc ctgacgctgg cgggttacta catgctggct
    44221 gtacaagtga tgctgatgct gccaattatg gtcaacgacg tggctggcgc accctctgcc
    44281 gttaaatgga tgtatgccat tgaagcgtgt ctgtcgttaa cgttgctcta ccctatcgcc
    44341 cgctggagtg aaaagcattt tcgtctggaa caccggttga tggctgggct gttgataatg
    44401 tcattaagca tgatgccggt gggcatggtc agcggcctgc aacaactttt caccctgatt
    44461 tgtctgtttt atatcgggtc gatcattgcc gagcctgcgc gtgaaacctt aagtgcttcg
    44521 ctggcggacg caagagctcg cggcagctat atggggttta gccgtctggg tctggcgatt
    44581 ggcggcgcta ttggttatat cggtggtggc tggctgtttg acctgggcaa atcggcgcac
    44641 cagccagagc ttccgtggat gatgctgggc attattggca tcttcacttt ccttgcgctg
    44701 ggttggcagt ttagccagaa acgcgccgcg cgtcgtttgc ttgaacgcga cgcctgattt
    44761 gatactcccc cctgactcct ccatactgaa agtgaactga cagccaaatg gaggagtcac
    44821 gtgaagctat acatttacga tcactgccct tactgcctca aagcccgcat gattttcggc
    44881 ctgaaaaata tccccgtcga attacatgtt ctgctcaacg acgacgcaga aacacccacc
    44941 cggatggtcg gtcaaaaaca ggttcccatt ctgcaaaaag atgacagccg ctatatgcca
    45001 gaaagcatgg acatcgttca ctatgtcgat aaactcgacg gcaaaccgtt actgaccggc
    45061 aaacgttccc ctgccattga agagtggctg cgcaaggtca atggctacgc aaacaaactg
    45121 ctgttgccgc gttttgccaa atcggcattt gatgagtttt ctactcccgc cgcgcgcaaa
    45181 tatttcgtcg acaagaaaga ggccagcgcg ggtaatttag ccgacctgct ggcccactct
    45241 gacggtctga ttaagaatat cagcgatgat ttgcgtgcgc tggacaaact gatcgtcaaa
    45301 ccgaacgccg tgaatggcga actttcggaa gatgatattc agctatttcc gctactgcgt
    45361 aatctgacgc tggtagccgg aatcaactgg ccaagccgcg ttgctgatta ccgcgataat
    45421 atggcgaaac agacacaaat caatttgtta tcatcaatgg cgatttaatc ctgacccgcc
    45481 gcggcagcct gtattcgcgg gttgccgccg ttttcagttt tttctgctgg cgagtttatc
    45541 gctggtgaca catgctgcgt tgtgtcagga tattgacgca gatttcatgt cgaggactac
    45601 catgaacaag tttttatttg ccgctgcatt gatcgtcagt ggcctgctcg ttggctgtaa
    45661 tcaactcacc caatacacca tcaccgaaca agaaattaac cagtcgcttg cgaaacataa
    45721 taatttctca aaagatatcg gtttacccgg cgtggctgac gcccatattg ttctgacaaa
    45781 cttgaccagc caaattggtc gcgaagagcc gaataaggtt accctaaccg gagacgccaa
    45841 tctggacatg aactccctgt tcggtagtca gaaagcgacc atgaaactga agctgaaagc
    45901 gctgcccgtg tttgataaag aaaaaggtgc gatcttcctg aaagagatgg aagtggtcga
    45961 tgcgacggta caaccggaaa aaatgcaaac ggtgatgcaa acgttgcttc cctatttgaa
    46021 ccaggcatta cgcaattact ttaaccagca acctgcttac gtcctgcgcg aagatggcag
    46081 tcagggcgaa gcaatggcga aaaaactggc gaaaggcatt gaagtgaagc caggcgaaat
    46141 tgtcattccg tttacggatt aatgacgagg gcgcattcgc gccctttatt tttcgtgcaa
    46201 aggaaaacgt ttccgcttat cctttgtgtc cggcaaaaac atcccttcag ccggagcata
    46261 gagattaatg actgcaccat cccaggtatt aaagatccgc cgcccagacg actggcacct
    46321 tcacctccgc gatggcgaca tgttaaaaac tgtcgtgccg tataccagcg aaatttatgg
    46381 acgggctatt gtaatgccca atctggctcc gcccgtgacc actgttgagg ctgccgtggc
    46441 gtatcgccag cgtattcttg atgccgtacc tgccgggcac aatttcaccc cattgatgac
    46501 ctgttattta acagattcgc tggatcctaa tgagctggag cgcggattta acgaaggcgt
    46561 gttcaccgct gcaaaacttt atccggcaaa cgcaaccact aactccagcc acggcgtgac
    46621 gtcaattgac gcaatcatgc cggtacttga gcgcatggaa aaaatcggta tgccgctact
    46681 ggtgcatggt gaagtgacac atgcagatat cgacattttt gatcgtgaag cgcgctttat
    46741 agaaagcgtg atggaacctc tgcgccagcg cctgactgcg ctgaaagtcg tttttgagca
    46801 catcaccacc aaagatgctg ccgactatgt ccgtgacgga aatgaacggc tggctgccac
    46861 catcactccg cagcatctga tgtttaaccg caaccatatg ctggttggtg gcgtgcgtcc
    46921 gcacctgtat tgtttaccca tcctcaaacg caatattcac caacaggcat tgcgtgaact
    46981 ggtcgccagc ggttttaatc gagtattcct cggtacggat tctgcgccac atgcacgtca
    47041 tcgcaaagag agcagttgcg gctgcgcggg ctgcttcaac gccccaaccg cgctgggcag
    47101 ttacgctacc gtctttgaag agatgaatgc tctgcagcac tttgaagcat tctgttctgt
    47161 aaacggcccg cagttctatg gcttgccggt caacgacaca ttcatcgaac tggtacgtga
    47221 agagcaacag gttgctgaaa gcatcgcact gactgatgac accctggtgc cattcctcgc
    47281 cggggaaacg gtacgctggt ccgttaaaca ataaaaatcg tagcttcctg ttgtcattag
    47341 gttattttac ctgtataaat aaccagtata ttcaacaggg ggctattatg cgaattgaag
    47401 tcaccatagc gaaaacttct ccattgccag ctggggctat tgacgccctg gctggcgaac
    47461 tttcccgccg tattcagtat gcgtttcctg ataatgaagg ccacgtatcg gtacgttatg
    47521 ccgcagcgaa taatttatcg gttattggcg caacaaaaga agataaacag cgcattagcg
    47581 aaattctcca ggaaacgtgg gaaagcgccg atgactggtt tgtcagcgaa taatatgcag
    47641 tgatttttgt ttttgccggg tcgccccggc ttttttattt tacttacaaa ttttgagtac
    47701 aaa
//
LOCUS       DAZFKM010000021        47149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFKM010000021 DAZFKM010000000
VERSION     DAZFKM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..922
                     /gene="ygcG"
                     /locus_tag="ACHKAY_001321"
     CDS             50..922
                     /gene="ygcG"
                     /locus_tag="ACHKAY_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5700029.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(982..2280)
                     /gene="eno"
                     /locus_tag="ACHKAY_001322"
     CDS             complement(982..2280)
                     /gene="eno"
                     /locus_tag="ACHKAY_001322"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO5700030.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2368..4005)
                     /gene="pyrG"
                     /locus_tag="ACHKAY_001323"
     CDS             complement(2368..4005)
                     /gene="pyrG"
                     /locus_tag="ACHKAY_001323"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO5700031.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4233..5024)
                     /gene="mazG"
                     /locus_tag="ACHKAY_001324"
     CDS             complement(4233..5024)
                     /gene="mazG"
                     /locus_tag="ACHKAY_001324"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO5700032.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5095..5430)
                     /gene="mazF"
                     /locus_tag="ACHKAY_001325"
     CDS             complement(5095..5430)
                     /gene="mazF"
                     /locus_tag="ACHKAY_001325"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO5700033.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5430..5678)
                     /gene="mazE"
                     /locus_tag="ACHKAY_001326"
     CDS             complement(5430..5678)
                     /gene="mazE"
                     /locus_tag="ACHKAY_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO5700034.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5756..7990)
                     /gene="relA"
                     /locus_tag="ACHKAY_001327"
     CDS             complement(5756..7990)
                     /gene="relA"
                     /locus_tag="ACHKAY_001327"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO5700035.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8038..9339)
                     /gene="rlmD"
                     /locus_tag="ACHKAY_001328"
     CDS             complement(8038..9339)
                     /gene="rlmD"
                     /locus_tag="ACHKAY_001328"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO5700036.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9396..12152
                     /gene="barA"
                     /locus_tag="ACHKAY_001329"
     CDS             9396..12152
                     /gene="barA"
                     /locus_tag="ACHKAY_001329"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO5700037.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12384..13724)
                     /gene="gudD"
                     /locus_tag="ACHKAY_001330"
     CDS             complement(12384..13724)
                     /gene="gudD"
                     /locus_tag="ACHKAY_001330"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO5700038.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13745..15085)
                     /gene="gudX"
                     /locus_tag="ACHKAY_001331"
     CDS             complement(13745..15085)
                     /gene="gudX"
                     /locus_tag="ACHKAY_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO5700039.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15087..16439)
                     /gene="gudP"
                     /locus_tag="ACHKAY_001332"
     CDS             complement(15087..16439)
                     /gene="gudP"
                     /locus_tag="ACHKAY_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO5700040.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16874..17323)
                     /gene="yqcA"
                     /locus_tag="ACHKAY_001333"
     CDS             complement(16874..17323)
                     /gene="yqcA"
                     /locus_tag="ACHKAY_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO5700041.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17341..18123)
                     /gene="truC"
                     /locus_tag="ACHKAY_001334"
     CDS             complement(17341..18123)
                     /gene="truC"
                     /locus_tag="ACHKAY_001334"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO5700042.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18123..18452)
                     /gene="yqcC"
                     /locus_tag="ACHKAY_001335"
     CDS             complement(18123..18452)
                     /gene="yqcC"
                     /locus_tag="ACHKAY_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO5700043.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19074..19619)
                     /gene="syd"
                     /locus_tag="ACHKAY_001336"
     CDS             complement(19074..19619)
                     /gene="syd"
                     /locus_tag="ACHKAY_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO5700044.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19687..20535
                     /gene="queF"
                     /locus_tag="ACHKAY_001337"
     CDS             19687..20535
                     /gene="queF"
                     /locus_tag="ACHKAY_001337"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO5700045.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20647..22011
                     /gene="ppnN"
                     /locus_tag="ACHKAY_001338"
     CDS             20647..22011
                     /gene="ppnN"
                     /locus_tag="ACHKAY_001338"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO5700046.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22568..23857
                     /gene="sdaC"
                     /locus_tag="ACHKAY_001339"
     CDS             22568..23857
                     /gene="sdaC"
                     /locus_tag="ACHKAY_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO5700047.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23915..25282
                     /gene="sdaB"
                     /locus_tag="ACHKAY_001340"
     CDS             23915..25282
                     /gene="sdaB"
                     /locus_tag="ACHKAY_001340"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO5700048.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25394..26149
                     /gene="xni"
                     /locus_tag="ACHKAY_001341"
     CDS             25394..26149
                     /gene="xni"
                     /locus_tag="ACHKAY_001341"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO5700049.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26204..27352)
                     /gene="fucO"
                     /locus_tag="ACHKAY_001342"
     CDS             complement(26204..27352)
                     /gene="fucO"
                     /locus_tag="ACHKAY_001342"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5700050.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27380..28027)
                     /gene="fucA"
                     /locus_tag="ACHKAY_001343"
     CDS             complement(27380..28027)
                     /gene="fucA"
                     /locus_tag="ACHKAY_001343"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO5700051.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28574..29890
                     /gene="fucP"
                     /locus_tag="ACHKAY_001344"
     CDS             28574..29890
                     /gene="fucP"
                     /locus_tag="ACHKAY_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO5700052.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29923..31698
                     /gene="fucI"
                     /locus_tag="ACHKAY_001345"
     CDS             29923..31698
                     /gene="fucI"
                     /locus_tag="ACHKAY_001345"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO5700053.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31807..33225
                     /gene="fucK"
                     /locus_tag="ACHKAY_001346"
     CDS             31807..33225
                     /gene="fucK"
                     /locus_tag="ACHKAY_001346"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO5700054.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33227..33649
                     /gene="fucU"
                     /locus_tag="ACHKAY_001347"
     CDS             33227..33649
                     /gene="fucU"
                     /locus_tag="ACHKAY_001347"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO5700055.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33707..34438
                     /gene="fucR"
                     /locus_tag="ACHKAY_001348"
     CDS             33707..34438
                     /gene="fucR"
                     /locus_tag="ACHKAY_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO5700056.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34482..35582)
                     /gene="rlmM"
                     /locus_tag="ACHKAY_001349"
     CDS             complement(34482..35582)
                     /gene="rlmM"
                     /locus_tag="ACHKAY_001349"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO5700057.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35575..35970)
                     /gene="ygdD"
                     /locus_tag="ACHKAY_001350"
     CDS             complement(35575..35970)
                     /gene="ygdD"
                     /locus_tag="ACHKAY_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO5700058.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35989..36906)
                     /gene="gcvA"
                     /locus_tag="ACHKAY_001351"
     CDS             complement(35989..36906)
                     /gene="gcvA"
                     /locus_tag="ACHKAY_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO5700059.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(37257..37484)
                     /gene="ygdI"
                     /locus_tag="ACHKAY_001352"
     CDS             complement(37257..37484)
                     /gene="ygdI"
                     /locus_tag="ACHKAY_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO5700060.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            37676..38881
                     /gene="csdA"
                     /locus_tag="ACHKAY_001353"
     CDS             37676..38881
                     /gene="csdA"
                     /locus_tag="ACHKAY_001353"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO5700061.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38881..39324
                     /gene="csdE"
                     /locus_tag="ACHKAY_001354"
     CDS             38881..39324
                     /gene="csdE"
                     /locus_tag="ACHKAY_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO5700062.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(39375..40181)
                     /gene="tcdA"
                     /locus_tag="ACHKAY_001355"
     CDS             complement(39375..40181)
                     /gene="tcdA"
                     /locus_tag="ACHKAY_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO5700063.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(40258..41355)
                     /gene="mltA"
                     /locus_tag="ACHKAY_001356"
     CDS             complement(40258..41355)
                     /gene="mltA"
                     /locus_tag="ACHKAY_001356"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO5700064.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41564..41640
                     /locus_tag="ACHKAY_001357"
     tRNA            41564..41640
                     /locus_tag="ACHKAY_001357"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41598..41600,aa:Met,seq:cat)
     gene            complement(41720..42667)
                     /locus_tag="ACHKAY_001358"
     CDS             complement(41720..42667)
                     /locus_tag="ACHKAY_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO5700065.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(42739..43335)
                     /locus_tag="ACHKAY_001359"
     CDS             complement(42739..43335)
                     /locus_tag="ACHKAY_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO5700066.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            complement(43304..44512)
                     /locus_tag="ACHKAY_001360"
     CDS             complement(43304..44512)
                     /locus_tag="ACHKAY_001360"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO5700067.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(44512..46092)
                     /locus_tag="ACHKAY_001361"
     CDS             complement(44512..46092)
                     /locus_tag="ACHKAY_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO5700068.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            complement(46124..46948)
                     /locus_tag="ACHKAY_001362"
     CDS             complement(46124..46948)
                     /locus_tag="ACHKAY_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO5700069.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
BASE COUNT        11887 a        12296 c        11044 g        11922 t
ORIGIN      
        1 accgatgccg ccgtcaattt atttatccag atttaatgag aaatttataa tgcgctattt
       61 catactaatg ttcactttcg tgtgttcctt tgttgcagcc caaccaacta ttgtcccaca
      121 attacagcaa caagttacgg atcttacgag tagcttaaac tcacaagaaa agaaagaact
      181 gacgcacaag ttagaatcta ttttcaataa tacccaagtg caaattgctg tattaattgt
      241 tcccacaacc aaagacgaga caattgaaca atatgctaca agagtttttg acaattggcg
      301 tttaggagat gccaaacgta atgatgggat actgatcatt gttgcctggt cagatcgtac
      361 tgtccgcatc caggtaggtt atgggctgga agaaaaggta accgatgctc tggctggaga
      421 tatcatccgt agcaacatga tacccgcatt taaacaacaa aaatttgctc agggattaga
      481 gctagctata aacgctttga ataaccaact cacttcacaa caccaatatc cgactaatcc
      541 ttcagaaagt gaatcagcgt cttccagtga tcattattac tttgctattt tttgggtatt
      601 tgcagtgatg ttcttccctt tctggttttt tcatcaaggc agtaattttt gtcgcgcatg
      661 taaaagtgga gtctatattt cagcaatcta tcttttagat ttattcctgt tctccgataa
      721 aattttttcc attgctgtat tttccttctt ttttactttc accatattta tggtctttac
      781 ctgtttatgt gtacttcaga aaagagcatc aggtaaaagt tatcattcag acaacagcgg
      841 ttccggtgga ggctcaagtg gcggcggatt tagcggcggt ggcggttctt ctggaggtgg
      901 cggcgcatcc ggccgctggt aactcaacca taaaaaatgc cagcccggag gctggcattt
      961 ttaaatcaga taaagtcagt cttatgcctg gcctttgatc tctttacgac cgttgtacgg
     1021 tgctttttcg cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc
     1081 agaacggctc atagaaccag ttttgatctg gcctgcagca gtaccaacag ccaggtcagc
     1141 gatggtagcg tcttcagttt cgccagaacg gtgagagata actgcagtgt agccagcatc
     1201 tttcgccatc ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat
     1261 caggatggag ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac
     1321 gaacaggtcg tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa
     1381 accgtcccag tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt
     1441 cagttcttcc aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc
     1501 cagaacgtat ttaccgtctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat
     1561 gtctttgccc agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc
     1621 agcgttggaa cccaggttcg gcgcatagcc accttcgtca ccaactgcag tgttcatgcc
     1681 tttcgctttc agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc
     1741 tttcacagtt ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc
     1801 agcgtgctca ccaccgttga tgatgttcat catcggaacc ggcatagagt atttgcccgg
     1861 agtaccgttc agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc
     1921 tttggcgtta gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc
     1981 ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc
     2041 aatcagcgcc tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc
     2101 caggaaacgg gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc
     2161 acctgacgga gcagctgcca taccgacgaa accaccctcc agatgtactt cggcttcaac
     2221 agtcgggtta ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat
     2281 taggttttcc tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaggg
     2341 tacgcgttgc cgttctaact tttttactta cttcgcctga cgtttctgga actcgctggc
     2401 ggctttcaca aagcctgcaa acagcgggtg accatcacgt ggagtagaag taaactccgg
     2461 atggaactgg caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg
     2521 atcatccccg gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat
     2581 gttgttgact tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg
     2641 gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc
     2701 gagatcgctc ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat
     2761 cagcgccaca accgggtact tacagtctgg cacaaattcc gtagagttgg cgttctccat
     2821 gttggcaaca tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag
     2881 ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg
     2941 atagccgaaa ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg
     3001 cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc
     3061 accgtgtttc agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc
     3121 gaccataccg atggtgactt cacttaccgg gttcgcttct tcgaagataa cctgttccca
     3181 ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag
     3241 cccctgagat ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat
     3301 aaccgctttt tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac
     3361 agcgcgatct gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt
     3421 tacagagtgc tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag
     3481 cgtcaggtgc ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatcgc
     3541 ttcgaggaac ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac
     3601 gtcatgacct tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat
     3661 aacctgcacg gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta
     3721 gatacgaccc gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta
     3781 gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg
     3841 gattgggctc atagtacctg gatcgacgtt gatgtacgga tccagtttca tgatggtcac
     3901 attgaggcca cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag
     3961 agaggatacg accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag
     4021 ttaggttgaa aagacgatgg aataaccagg acgggaaagc agtatacccg aacatgacct
     4081 gtgccacaaa ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag
     4141 aaaatagccc ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa
     4201 tgacacaaat ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc
     4261 tgttgccaga cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg
     4321 gcaacaatac gctccacttc gcgaaaacga cgctcaaatt tttcgttcgc tttttgcaat
     4381 gcgatttccg cttttgtccc taaatgacga gccagattga ccgtggcaaa cagcaggtcc
     4441 cccatttcct cctccagttt agcctggtcg acaacagcct gccgcgcttc gtacatcacc
     4501 tcgtcgatct cttcgtagac tttatcgact accggaccaa gcgtcgtcca atcgaagcca
     4561 acgttggcgc aacgtttctg gattttttgc gcacgcatta aagccggtaa actacgagga
     4621 atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc
     4681 caacgggcaa gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg
     4741 cgacgctcta atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct
     4801 tcctgagcca tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga
     4861 agatcgtcaa aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg
     4921 taaggcgcaa tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg
     4981 cgcaggcgct gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa
     5041 ttgcgggtct gtcaggtgga aacctgtgac cagaatagaa gtgagttagt aacactaccc
     5101 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt
     5161 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc
     5221 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtata
     5281 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc
     5341 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc
     5401 gcccatatcg ggtacgtatc ggcttaccat taccagactt ccttatcttt cggctctccc
     5461 cagtcgatat tctcgtggag gttttccggc gtgatgtcgt tgaccagttc agcaagcgta
     5521 aatacgggct ctttacgcac tggctcaata attaatttgc catccaccag gtcaatcttc
     5581 acttcatcat caatattcag attgagcgcc tgcattaacg tagccgggat ccgcaccgcc
     5641 ggtgaatttc cccaacgctt tacgctactg tggatcataa ccctttcctc aaaccgctat
     5701 catatgtaga tacagtatat atcaatctac attgtagata cgagcaaatt tcggcctaac
     5761 tcccgtgcaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc
     5821 gccccagcac ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt
     5881 tggtgtcgct acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg
     5941 tgatatcacg taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc
     6001 cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca
     6061 gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgcccc tgggtaatga
     6121 agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt
     6181 tgccaacacc ttcgaccacc acgcgaccgt tatctttact gcggttttgc ggcgtgtagc
     6241 ttttttgctg aagttgcttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa
     6301 attgcgattg caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg
     6361 ccagcaactc gtcgacatca ttaaagttgt aacgcggcag cagatgtttt tctgcttctt
     6421 tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt
     6481 ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg
     6541 tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga
     6601 taatttcaat ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa
     6661 tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg
     6721 gcgttgatcc cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca tacacccggt
     6781 cgtcaaagac ctggctgcgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct
     6841 gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc
     6901 cagctgccgc gccctcttta tatttccagt gcgcagcaac acccaactct gcatcttcat
     6961 gcatctgttt ggtgcggatt tggatctcaa cggttttgcc acctggcccc agaaccacgg
     7021 tatgaataga ctgataaccg tttggtttcg ggttagcgac ataatcgtca aactcatccg
     7081 gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct
     7141 cggcgacaat acgtaccgca cgcacatcaa acagctcatc gaaggcgagg ttctttttct
     7201 gcattttgcg ccagatactg tagatgtgtt tcggacgacc atacacttca gctttaacgc
     7261 cttcagcttt catctcagcg cgcagatggc cgacaaactc ttcgatatag tgttcgcggt
     7321 cgagacggcg ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg tggagataac
     7381 ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg ttcgccaacg
     7441 gcgcgtagat attggtgcac tcttttgccg ccagtacgcg ttcatcttcc ggcgcatctt
     7501 ttacttcgcg cagatgagca atacgctccg ccagtttgat gaccacgcag cgaaaatcat
     7561 cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag
     7621 tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa
     7681 cgaccgactt accgacgctc tcacgcagca catcttcgct gactacgttg gcatcagcca
     7741 gagggaacag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct
     7801 ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt
     7861 gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg gtaataccca
     7921 gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta
     7981 ccgcaaccat tgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgtaacta
     8041 tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag
     8101 catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag
     8161 cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta taatttgctg
     8221 cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt
     8281 cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg
     8341 taaaccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac
     8401 accgaccaca ctggctgctt gtttcgccaa tggcagtgta aagttgccca taccgcagaa
     8461 cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat
     8521 tttttggttc acacccgcat tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa
     8581 cccgtttgag tcataccagg gcatctcacc agagacggtt tcgagtatct cactatcggg
     8641 ggcgagatac agatccaggc cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc
     8701 cgaacttagc ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag
     8761 ttcaacatga ccaagatggc gcataacttg taagctgccc aggcatgccc tgactttggg
     8821 cagcaatgct tcaagttggg gcactaaaat ggggcattgt ttaacgtcga caatgtcact
     8881 ggagcccgct ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt agttcagact
     8941 taaacgcgcg cggcggcgat agccccaggg aacatcggcg atcacttcag agacatcgtg
     9001 tttcattaat cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg
     9061 ttgttgctga cagccgccgc atacgccaaa atgaggacag cgtggtgttt cgcgttccgg
     9121 gctatcgctt aaccggcgta cgactttagc gcgggcatac tgttttttat cttcagtaac
     9181 agcaacttcc gcgttttcct gcggcaataa tccggggata aataacgttt tgccgttatg
     9241 tcgcgccacg ccctgaccaa aagagtcgag gtcgttgact gaaacggtta tgatctgacg
     9301 cgtcgtcgtg cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt tctcaattta
     9361 acagtgtgac cttaattgtc ccataacgga actccatgac caactacagc ctgcgcgcac
     9421 gcatgatgat tctgatcctg gcaccgaccg tccttattgg tttattgctg agtatctttt
     9481 tcgtcgtgca tcgctataac gacttgcagc gtcaactgga agatgccggt gccagcatta
     9541 ttgagccgct tgcagtttct actgaatatg gcatgagcct gcaaaatcgc gaatctatcg
     9601 gtcagttaat aagcgtactg catcgtcgcc attccgatat tgttcgcgcg atttcggttt
     9661 atgatgaaaa taaccgactc tttgtcacct ccaattttca tcttgatccc tcatcaatgc
     9721 agctcggcag caacgtgccg tttcctcgcc agctcactgt cactcgtgac ggcgatatta
     9781 tgatcctccg cacgccgatt atttctgaga gttactcccc cgacgaatcg cccagtagcg
     9841 atgccaaaaa tagtcaaaat atgttgggat atattgcgct ggagctggat cttaaatcgg
     9901 ttcgcttgca gcaatataaa gagatcttta tttccagcgt gatgatgctg ttttgtatcg
     9961 gtattgcgct tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca
    10021 tggtgaatac cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg gaaggattta
    10081 tgctcggcga gctggatatg ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg
    10141 cttatcacga agagatgcag cacaatatcg accaggcgac gtccgatctg cgtgaaacgc
    10201 tggagcagat ggaaattcag aacgttgagt tagatctggc gaaaaagcgc gcccaggaag
    10261 cggcgcgtat taaatccgag tttctggcaa atatgtcaca cgagctgcgt acaccactga
    10321 atggtgttat tggctttacc cgcctgacgc tgaaaacaga attaacacca acgcagcgcg
    10381 atcacctgaa tacgattgaa cgttcggcaa ataatttgct ggcaattatt aatgatgttc
    10441 tcgacttctc gaaactggaa gcaggtaagc tgattctgga aagtattcca ttcccactac
    10501 gcagcacgct ggatgaagtc gttactctgc tggcacattc ttctcacgat aaagggttgg
    10561 aactgacgct caatattaaa agcgacgtgc ctgataacgt gatcggcgac ccactgcgat
    10621 tacagcaaat catcactaac ctggtgggga atgcaattaa attcaccgag aatggcaaca
    10681 ttgatattct ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga
    10741 ttcgggatac cggcattggt attcctgaac gcgatcaatc gcgcttattc caggccttcc
    10801 gacaggctga tgccagtatt tcccgccgtc atggtggcac cggtctgggg ctggtgatta
    10861 cacaaaaact ggttaatgaa atgggcggcg atatttcgtt ccatagccag ccgaatcgcg
    10921 gttcaacttt ctggttccac attaatctcg atctgaaccc gaacattatt atcgaagggc
    10981 catccaccca gtgcctcgcc ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag
    11041 cgcaatgcac gctggatatt ttaagtgaaa cgccgctgga agtggtttat agcccaacgt
    11101 tctccgcgct gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc
    11161 gcgagccgct aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg accgatttcc
    11221 tgatgctggc acttccttgc catgcacaag tcaatgcaga aaaactcaag caagatggta
    11281 tcggcgcgtg tctgctgaaa ccattaacac ctacgcgcct gttgcctgcc ctgacggaat
    11341 tttgtcatca caaacaaaac acgcttttgc ctgtaaccga tgaaagcaag ctggcaatga
    11401 cagtcatggc agttgatgac aaccccgcta acctgaaact tatcggcgca ttgctggaag
    11461 atatggtgca acatgtggaa ctttgcgata gcgggcatca ggcggttgaa cgggcgaaac
    11521 agatgccgtt cgatttgatc ttaatggata ttcaaatgcc tgacatggat ggcattcggg
    11581 cctgcgagct catccaccag ctcccccatc agcaacaaac gccggttatc gcggtaacgg
    11641 cgcatgcaat ggccgggcaa aaagagaagc tgcttggcgc agggatgagc gattatctgg
    11701 cgaaaccgat tgaagaagag cgattgcata atttgttgtt gcgctacaag cctggcagcg
    11761 gtatttcctc tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga
    11821 ccctcgactg gcaactggca ctacgccagg cagcaggaaa aaccgattta gcgcgcgata
    11881 tgctgcaaat gttactcgat ttcctgcctg aagttcgcaa caaagttgag gaacagctgg
    11941 ttggagaaaa cccggaaggc ctggttgatt tgattcataa actgcatggc agttgcggct
    12001 atagcggtgt gccgcgtatg aagaatctct gccaactgat cgaacaacag ctacgtagcg
    12061 gtactaaaga agaagatttg gagccggagc tgctggaact gttggacgag atggataatg
    12121 tagcgcgcga agccagcaaa attctcgggt aacggacgtc ttgtcgagta gcaatcggct
    12181 ttttatgacg ctggtttcag attggtgaca aagtgcctgt gtttatgccg gatgcggcgt
    12241 taacgcctta tccggcctac aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac
    12301 ctgatacata gcacaacagg ctattttgcg tttagcatca gtctcaagcc ggctccagat
    12361 agagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc
    12421 cagccaggaa tcagatactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc
    12481 tgatacagct catgggcttt catcacttga tccatatcga tttctacacc cagccccggt
    12541 ttttccggca cctgaaccag accgcctttg atctcaaacg gttctttggt caggcgctga
    12601 ttaccttcct gccagatcca gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg
    12661 gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgtttgagtg tgaaccccag
    12721 gtcaggccaa attcatggca catttgcgcc acacgtaccg aaccttgcat cgtccagaaa
    12781 tgcggatccg ccagcgggat atcaacggat tgcagggaga gcgtatggcc catctgccgc
    12841 cagtcggtgg cgatcatatt ggttgcggtc ggcaggccag ttgcgcgacg gaactcagcc
    12901 atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagc
    12961 gaccctttca ggtatttacc aattttaatc gcttcgttca gcgaccaggc accgttagga
    13021 tcgagcgtaa tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga ctcggcctct
    13081 tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt tgaagccata tttttcatac
    13141 gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga
    13201 taccagtcgc atgagtcatc cggctggctt tgatacggca gcggtgtggc tttgcgatca
    13261 ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc atcgcccagc
    13321 agcgatgcca cgtttacccc cagatgctgc cccagcagat ccagcattgc cgcttctatc
    13381 ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccagca
    13441 tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt ttttgtattt acccagcgtt
    13501 ttacctacca ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga
    13561 atttccccta cgccagtgtg accagaatta tctttgataa tcacaatatt acgcgtaaag
    13621 aacggtgcgt gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg
    13681 acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc
    13741 agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg gggatcaggt
    13801 actgcatcgg acctgcgtcg ttacgcgcac cgccaggcag acgtttatag gcctcatgtg
    13861 ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa
    13921 tttttccgtt tttaatctcc agcggatttt tggtcaggcg acaatcgccc tcctgccaaa
    13981 tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca
    14041 tcgccagaga gatatcgaaa tggttattag aatggcagcc ccaggtcagc ccccagtcgt
    14101 cgcaaagctg cgccacacgg actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg
    14161 gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcgatca
    14221 tgttagtcgc gacgggcaag ccggtcgccc gccgaaactc cgccatcact tcacgaccgg
    14281 agaaaccctg ttctgcgccg catggatctt cggcataggt aagaacatca ttcagccctt
    14341 tgcatagaga aatggcttca tcaagtagcc aggcaccgtt gggatcaacg gtaatccgcg
    14401 catcagggaa gcgtttcttc aatgcccgaa cagtgtcgat ttcttgctcg ccaggtaaca
    14461 ccccgccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac
    14521 gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac tcatggttgc
    14581 ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcaccgata taaaacagat
    14641 aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc caacagttcg cagaccggaa
    14701 cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc acggcattaa
    14761 cgcgcaattc aaaagtccag gcacctttgc cgaaggtatc aaaatcggct gcctgattac
    14821 ctttatgcac ctgctgaacc actttattca ggcgcgcaac ttcctggccc agaaccatcg
    14881 gaatagcatc gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg
    14941 tatgcccggc gttatcggtg agtaccacaa tattgcgggt gaaatatgcg ttatgtgccc
    15001 caccaatatt aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc atatcagtaa
    15061 taacaggact ggattgtgtc gtcatcttat tgccccgcga caggtttcag ctcgatacgc
    15121 ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttaaggcatg aacaccaaca
    15181 taaatcagcg ccccattaaa ggagccagtc gtgccaacga tataaccaat tgcgattggc
    15241 gtgacgatgc cagaaatgtt accgaacatg ttgaacaggc caccggaaag accgctgatc
    15301 tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc cttaccgaag
    15361 aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc
    15421 atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat gttcagcgat
    15481 cccgtgcggc gcatcagcca gtcggaaata atcccaccca gcacaccgcc gataaaaccg
    15541 caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc acgcgcctgc
    15601 accagataaa ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat acagtactga
    15661 ccgatataaa cgccgatcat catccgtgac ccgagcagtt gtttgatctg cccccacttc
    15721 acgctgaacg gcactttaac tttggtgttt tgctgatcca tattgatcag cgcgccaccc
    15781 gcggcgatgt actccagctc tttctgattt acccccggat gttgatttgg ctcgtggatg
    15841 actttcaacc agataaagct gatgacaatc cccagaccgc ccataaagaa gaagacgtgt
    15901 gaccagccca cttcatgcgt cagccagccc ataatcggcg cgaagatcac cgttgcgaag
    15961 tattgagcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc
    16021 gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac caggaagcgc
    16081 agcgtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc
    16141 aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacga
    16201 tccagcaacc aaccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac
    16261 acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga accagcaata
    16321 gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc
    16381 cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt
    16441 gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca tcgccaggaa
    16501 atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg aatacggcgc
    16561 gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac cgtgcaacaa
    16621 cacaacatta atgcgttcaa tgaggcccga ttttggcatt agccctggac ggtggaatcc
    16681 acttcacgga aatgaaaaca agaacaagaa aggaagggta aaaacgaaga aataaagaag
    16741 agtatgaaat ggatcgcttg actccaggca aacgccagta aaaatccgcg ctatgaagca
    16801 gtttttactg gcatttgcct gaaaagattc gattcagcac cgctaaaacg acatttaccg
    16861 ctcgctgaac atatcaggac aacagcgtgc cccactgttc gacccacgga tttgattccg
    16921 tttccggttc cgggttctca ctggcgtcaa tcaacagtat ttcgccaacc cgctgagcgc
    16981 tctgttcctg caacaaggca tcgaactgtt tgccgccatt gcagaaattt acataactgc
    17041 tgtcgccgag cgcaatcacg ccataacgca gattcggctg gaagcccaga ctatctttga
    17101 ttccctgaaa gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc gtggacgtaa
    17161 ccaccagaac atacttatcc tgatagggca gccagtcgct taattcagga tcttcaaata
    17221 ccgttgcttt gtggccctgt gcggtcagaa ttgcttctgc ttcttccgcc actaacagcg
    17281 aatttccgta catggtgccg acaaaaatac caatttccgc cataccgttt tctccctgga
    17341 ttaggaactt atctctccat cctgacccga tggcgcactg aactcaaccc tttcattttc
    17401 aggaagcaga ccgcgccagc caaattgtga taacgcctgc atccaggtgt cgtccaggcc
    17461 cgcgtgaata gtcagcggct cgccagtaaa aggatgcgtc aatgacaact gactggcatg
    17521 tagcattaac cgctggaggc caaaatgctc agcaccgctg cgattctggc gtaaatcgcc
    17581 atgtttgcta tcgccaataa tcggatgacg caaatgggca agatgtcggc gaagctgatg
    17641 tttgcgtccg gttttcggct ccagttccac caggccgtag cgcgtggtcg ggtaacgtcc
    17701 ggtcgctacc ggcatttcta cggtcgccag accgcgataa tgcgtcactg ctggctgcga
    17761 gcctttatct tcgcgggcaa atttatcagc gattttgtcc agttcttcca ccagtggata
    17821 atccagaact gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac gtttctggat
    17881 ttggtgctgt tcaaactgtt gtgccagcag ccgtccggcc tcgctggata atcccatcaa
    17941 caacacacca gaagtgggtc ggtccagacg atgagcggta aaaacgtgct ggccaatctg
    18001 atcacgcacg gtttgcatga ccactacttt ctcgtcgcga tccagccagc tgcggtgaac
    18061 cagccagccg gagggtttat ttaccgcaac cagccattca tcctgataga gtatttccag
    18121 cattagctcg catcatccgc aaaaagagca tccagttttt ccagctcagc cagaataagc
    18181 gcgcgttgcg gatggtccgt cgccagcgcc atttcataat agggtgcaac ggcaaaagcg
    18241 cccggtaacg gctgtttgtt atctaacaaa tcgtgcattc gcgggatcag cacccactgc
    18301 aaccactcca gtggttccat ggtgtccata aagaacggtt gggtactatt aaattgatgc
    18361 ggctggggtt catcgtttcg ccagtgctga tgttcacgca gtaacgcttc aagcgcctgc
    18421 aactggagac gaacgcggtc atgagtggtc ataaagcaac ctcaataaga aaaactgccg
    18481 cgaaggatag caggaataaa aaaagggagc actgtattca cagcgctccc ggttcgtttc
    18541 gcagcattcc agctactttt gttgctccct gctcatcctt gacaactttt cctctggcct
    18601 tgcggccaat cgttcatcct gaactattgc ttcctgctca caccaccccg atgtgatact
    18661 tcatcctgaa gtgtccctgg ccttcctgac ccaccgaatc atcctgaccg gttctcattc
    18721 tccatcctgg aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc atccagaagc
    18781 ctttccctga aacaccatcc tggtgtgtcc tgcagaagtg tcatcatcct gatgttcact
    18841 tcgttgtctg actccctgtc gacgaagata gaatcgtctt tttcgcgaag tcttacaagg
    18901 cgcttacaga caatgcctta aggaaaattt catatgaaag ttaagaatta acaatcaaca
    18961 ttttgatttt aaatgaaaat aacgattctt atctttttaa gtattcgctt atttcctggc
    19021 gatctcctac agagtctgta agagatctct cacaaccaga tggggtattt ggattacaga
    19081 agaggcttaa gttgattaag gaattccgca agatttgagg ctaaatgcgt ccgtttacgt
    19141 gttcccagcg tctctttgca cacttcaccg gagagattac acaccgagat gacctctaac
    19201 tcttcttcca gcgtcgcgat aaatagcgtg ggcggcagtt ttagcctttt ctgagtcacc
    19261 agatgaccaa tcagattttc ctgcacccga cggaagtcgt cttcactcca ggtttgcagc
    19321 aatgtcagtt tgatatcgcc aaactgcgcg tgcatatccc cggcaaactg agtcgtataa
    19381 aaggtatgaa ttgtgggttg tatcacaata tcaaaagcgc gttcaaccgc gtttacattt
    19441 tgttccccgg taaacggctg gggttgccag tatacggcat cttcggtagt tgaaataatg
    19501 cacggtgaag gaacaccgta cagttcctca cttaacggcc agcttttatg ctcttcatgc
    19561 cacgcatcgc agtagcgtgc agtaaaatct ttcagggctt gtgcggtcaa atcgtccact
    19621 aatttctctc ttcacttaaa ccagatacac ttgtcgttta gtttatctgg tttatgacgg
    19681 tgaaacatgt cttcttatgc aaaccatcag gcacttgcgg gcctgactct tggaaaatca
    19741 accgattacc gggataccta tgacgccaac ctactgcaag gcgttccacg cagcctgaat
    19801 cgcgacccgc tgggtctgaa agcggataac ctgccttttc acggtacgga tatctggacg
    19861 ctgtatgaac tttcctggct gaatgcgaaa ggtttgccgc aggttgccgt cggtcacgtt
    19921 gaacttgatt acaccagcgc aaatctgatt gagtcgaaga gttttaagct ctatctcaac
    19981 agttttaacc agacgcgttt taataactgg gatgaggtgc gccagacgct ggagcgcgac
    20041 ttaagcactt gcgcccaggg taaggttagc gtggcgttat atcgtcttga tgaactggaa
    20101 ggccagccga taggtcattt caatggcacc tgcattgatg accaggatat cactatcgat
    20161 aactatgaat tcactactga ctatctggag aatgccacca gcggtgaaaa agtagtggaa
    20221 gagacgcttg tcagccacct gctgaaatca aactgcctga tcacccatca accagattgg
    20281 ggttcgatcc aaattcagta tcgtggacgc caaattgaca gagaaaaact gctgcgttac
    20341 ctggtctcat tccgtcatca caacgagttc cacgaacagt gcgtggaacg catctttaat
    20401 gacctgttac gcttctgcca gccagagaaa ttaagcgttt acgcacgtta tacccgtcgt
    20461 ggcggtctgg acattaaccc gtggcgcagt aatagcgatt ttgtcccatc gaccacaaga
    20521 ctggttcggc aataaatttt ttctcaattt tgcgtgctgg attcacgcag aaggttgtga
    20581 aaggtcatca ggcagggcta ttgtaatcaa agggaatgac gatattcgtc ccataaggag
    20641 tttttcttga ttacacatat tagcccgctt ggctccatgg atatgttgtc gcagctggaa
    20701 gtggatatgc ttaaacgcac cgccagcagc gacctctatc aactgtttcg caactgttca
    20761 cttgccgtac tgaactccgg tagtttgacc gataacagca aagaattgct gtctcgtttt
    20821 gaaaatttcg atattaacgt cctgcgccgt gaacgcggcg taaagctgga actgattaat
    20881 cccccggaag aggcttttgt cgatgggcga attattcgcg ctttgcaggc caacttgttc
    20941 gcggttctgc gagacattct cttcgtttac gggcaaatcc ataataccgt tcgttttccc
    21001 aacctgaatc tcgacaactc cgtccacatc actaacctgg tcttttccat cttgcgtaac
    21061 gctcgcgcgc tgcatgtggg tgaagcgcca aatatggtgg tctgctgggg cggtcactca
    21121 attaacgaaa acgagtattt gtatgcccgt cgcgtcggaa accagctggg cctgcgtgag
    21181 ctgaatatct gcaccggctg tggtccggga gcgatggaag cgccgatgaa aggtgctgcg
    21241 gtcggacacg cgcagcagcg ttacaaagac agtcgtttta ttggtatgac cgagccgtcg
    21301 attatcgccg ctgaaccgcc taacccgctg gtcaacgaat tgatcatcat gccagatatc
    21361 gaaaaacgtc tggaagcgtt tgtccgtatc gctcacggta tcattatctt ccctggcggt
    21421 gtgggtacgg cagaagagtt gctgtatttg ctgggaattt taatgaaccc ggccaacaaa
    21481 gatcaggttt taccattgat cctcaccggc ccgaaagaga gcgccgacta cttccgcgta
    21541 ctggacgagt ttgtcgtaca tacgctgggc gaaaacgcgc gccgccatta ccgcatcatc
    21601 attgatgacg ccgctgaagt cgctcgtcag atgaaaaaat cgatgccgct ggtgaaagaa
    21661 aatcgccgcg acactggtga cgcttacagc tttaactggt caatgcgcat tgcaccagat
    21721 ttgcaaatgc cgtttgagcc gtctcacgag aatatggcta atctgaagct ttacccggat
    21781 caacctgttg aagtgctggc tgccgacctg cgccgtgcgt tctccggtat tgtggcgggt
    21841 aacgtaaaag aagtcggtat tcgcgccatt gaagagtttg gtccttacaa aatcaacggc
    21901 gataaagaga ttatgcgtcg tatggacgac ctgctacagg gttttgttgc ccagcatcgt
    21961 atgaagttgc caggctcagc ctacatccct tgctacgaaa tctgcacgta atctcccctc
    22021 ccccggtgac gtttgttgcc gggttttctc gtttttggtc acttactcat caactcattt
    22081 catttgttat atgaatgttt cttaccaccc tcacgcgaca aatatcatca cagttaatat
    22141 gtcatacaat ttatattgca acgcaaacgt ttccctattt tcataaaccg tattttatcg
    22201 ctatgaaaaa gaaattatcg ccatgattaa ctaaaagtat tgattttttc agttcaacct
    22261 acatatattg cgcgccccgg aagaagtcag atgtcgttta atgggcaaat attgccctta
    22321 aattctcttt tacttttgat ttacagagta aagcgttggg ataatctatc ttccaagtag
    22381 attattgtat ttgggatcaa gatcactgat agatacataa cttgtgtgta tctttccgcc
    22441 ctcaaattat tacggcggta aatgattaag ccatcgccga tagacagatt tcatttttac
    22501 ggtcaggcac cttcccgggc tgaactggct aaaagctgaa ttatttgcat tcctccagga
    22561 gaaatagatg gaaacgactc aaaccagcac gattgcgtcg aaagactctc gtagtgcctg
    22621 gcgcaagaca gacaccatgt ggatgctggg cctttacggc acggcaatcg gcgcgggcgt
    22681 gctgttcctg ccaatcaacg ccggtgttgg cggtatgatc ccgctgatca tcatggctat
    22741 ccttgcgttc ccgatgacgt tttttgctca ccgcggcctg actcgcttcg tactgtctgg
    22801 taaaaacccg ggcgaagaca tcaccgaggt tgtagaagaa cactttggta ttggcgcagg
    22861 taaactgatt accctgctct acttcttcgc tatctacccg atcctgctgg tttatagcgt
    22921 ggcaatcacc aatacagttg aaagcttcat gtctcaccag ctgggtatga cgccaccgcc
    22981 gcgtgcgatt ctgtcgctga tcctgatcgt gggtatgatg accatcgttc gctttggtga
    23041 gcagatgatc gttaaagcga tgagtattct ggtattcccg tttgttggcg tactgatgct
    23101 gctggctctg tacctgatcc cgcagtggaa cggcgcagca ctggaaacgc tgtctctgga
    23161 cactgcatct gcaaccggaa acggtctgtg gatgaccctg tggctggcaa ttccggtaat
    23221 ggtgttctcg ttcaaccact ctccgatcat ctcttctttc gccgttgcga agcgtgaaga
    23281 gtacggcgat atggcagaac agaaatgctc gaagatcctg gcattcgcac acatcatgat
    23341 ggtgctgacc gtaatgttct tcgtcttcag ctgcgtactg agcctgactc cggcagacct
    23401 ggctgcggct aaagagcaga acatctcgat tctgtcttac ctagctaacc actttaacgc
    23461 accggttatc gcgtggatgg ctccgattat cgcgattatc gctatcacca aatccttcct
    23521 cggccactac ctgggcgcac gtgaaggctt caacggtatg gtgattaaat ctctgcgtgg
    23581 taaaggtaag tctatcgaaa tcaacaagct gaaccgtatc actgcgctgt tcatgctggt
    23641 aacgacctgg attgttgcca ccctgaaccc gagcatcctg ggtatgattg aaaccctggg
    23701 cggcccaatc atcgcgatga tcctgttcct gatgccgatg tacgcaattc agaaagtacc
    23761 ggcaatgcgt aagtacagcg gtcacatcag caacgtattc gttgtcgtga tgggtctgat
    23821 tgcaatctcc gcaatcttct actctctgtt cagctaagtc ctttcgcgcc gctttcgggc
    23881 ggcgcttcct ccgttttaac gcgatgtatt tcctatgatt agcgtattcg atattttcaa
    23941 aatcggcatt ggcccttcca gttctcatac cgttggacca atgaaagcgg gtaaacaatt
    24001 taccgacgat ctgattgccc gtaacctgct taaagacgtg acccgcgtgg tggttgacgt
    24061 gtacggctcg ctctctctga ctggtaaagg ccaccacact gatatcgcca ttattatggg
    24121 cctggcgggt aacctgccgg ataccgtgga tatcgattcc atccctggtt ttattcagga
    24181 tgtgaatact catggtcgcc tgatgctggc aaacggtcag catgaagtgg agttcccggt
    24241 tgatcagtgc atgaacttcc atgcagacaa cctttctctg catgaaaacg gtatgcgcat
    24301 taccgcgctg gcgggcgata aagtcgttta cagccagact tactactcta ttggcggtgg
    24361 ctttatcgtt gatgaagagc attttggcca gcagaatagc gcaccggttg aagttcctta
    24421 tccgtacagt tcagcagccg atctgcaaaa acattgtcag gaaaccgggc tgtcactctc
    24481 tggcctgatg atgaaaaatg agctggcgct gcacagcaaa gaagagctgg aacagcacct
    24541 ggcgaacgta tgggaagtca tgcgtggcgg tattgagcgc ggtatttcca ccgaaggcgt
    24601 gttgcctggc aaactgcgcg ttccacgccg tgctgcggca ctacgccgga tgctggtcag
    24661 ccaggataaa accaccactg acccgatggc ggttgttgac tggatcaaca tgtttgcatt
    24721 ggcagtgaac gaagagaacg ctgctggcgg acgcgtggtg actgcgccga ctaacggtgc
    24781 gtgcgggatt atcccggcag tgctggcgta ctacgacaag tttatccgcg aagtgaacgc
    24841 taactcactg gctcgttacc tgctggtagc cagtgccatt ggttctcttt ataagatgaa
    24901 cgcgtcaatt tctggtgctg aagtcggctg ccagggtgaa gttggtgtgg cgtgctcaat
    24961 ggcggcggct ggtctggcag aactgttagg tgcaagcccg gcgcaggtgt gcatcgcggc
    25021 ggaaatcgcc atggagcaca acctcggtct gacgtgtgac ccggtcgccg gacaggtcca
    25081 ggtgccatgc atcgagcgta acgccattgc ggcagtaaaa gcggtgaacg ccgcacgtat
    25141 ggcgctacgc cgtaccagcg agccgcgcgt ctgcctcgat aaagttatcg aaaccatgta
    25201 cgaaacaggt aaagatatga acgccaagta ccgcgaaacc tctcgcggcg gcctggcaat
    25261 gaagatcgtt gcctgcgatt aatcgctctc cagaggcctc gttttgcgag gcctcttccc
    25321 gatttctcat ccagccgtag cctgttccgg catcgaatgt tacccttatc gcctgatctt
    25381 taagggggtt atcgtggctg ttcatttgct tattgtcgat gcactgaatc ttattcgtcg
    25441 cattcatgcc gttcagggat cgccctgtgt cgaaacctgc cagcatgcgc tcgatcagct
    25501 cattatgcac agccagccaa cccacgcggt cgccgttttt gatgatgaaa accgcagtag
    25561 cggctggcgt catcagcgtt taccagatta caaagcgggt cgaccgccaa tgccggaaga
    25621 gttgcacgac gagatgcctg cattacgcgc cgcctttgag caacgcggcg tcccgtgctg
    25681 gtcaaccagc ggcaacgaag ccgatgactt agccgccacg ctggcggtca aagtgacaca
    25741 ggccgggcat caggcaacga ttgtttcgac agataaaggc tactgtcagt tactttcacc
    25801 gacattacgt attcgtgatt acttccagaa acgttggctg gatgcgccat ttatcgataa
    25861 agaatttggc gttcaaccgc agcagttgcc cgattactgg ggacttgcgg ggatcagcag
    25921 ttcaaaggta ccgggtgttg cgggaatcgg accaaaaagc gccacgcagc tgctggtcga
    25981 gtttcagagt ctggaaggga tatatgagaa tctggatgcg gttgccgaaa agtggcgcaa
    26041 aaaattagaa acccataaag agatggcgtt tctgtgccgc gatattgccc gcttacaaac
    26101 cgatttgcat atcgacggca atttacagca attgcggttg gtacggtaac ggcgagccgg
    26161 atacgccgca aacgtcgtat ccggcattat cacatcagcg catttaccag gcggtatggt
    26221 aaagctctac aatatcctca agcgttgctt cacgcgggtt gccaccggta caaacatcat
    26281 ccagtgccgc ctgcgccagt gccggaatgt cttccttgcg taccccaaca tcacgcaaat
    26341 gtggcggaat accgacatca cggttgagag caaacaccgc ttcaacagcg gcattacgcg
    26401 cctcttccag gctcatacct tccactttca cgcccataac gcgcgcgata tcgcggtact
    26461 tctcaccggt aaagtcagcg ttatagcgca tgacatgcgg tagcaggatg gcgtttgcaa
    26521 caccgtgtgg agtgttataa aacgcgccca gtggatgcgc cataccatgc accaacccta
    26581 acccaacatt cgagaagccc atacccgcaa catactgccc gagcgccatt tcttctccgg
    26641 catccttatc accagcaacc gatcctcgca gcgccccagc aatgatttca atcgctttaa
    26701 tgtgcagtgc atcggttagc gcccacgcgc cacgggtaat atacccctca atagcatgag
    26761 tgagcgcatc gacacccgtc gcagctttca gcgctggagg cataccatcc atcatgtcag
    26821 cgtcaataaa cgccacctgc gggatatcat gcggatcaac gcaaacaaac ttgcgccgtt
    26881 tttcttcgtc agtgatcacg tagttaatgg tcacttctgc cgcagtgcct gctgtggtgg
    26941 ggattgccag aatcggtaca ctgggtttat tggtcgggga aagtccttcc aggctacgca
    27001 catcggcaaa ctccgggttg ttgctgataa tgccaatcgc tttacaagta tcctgtggag
    27061 aaccaccacc aatagcgatc aggtaatccg cgccgctatt ctggaataca ccgagccctt
    27121 ctttgacgac agtaattgtt gggttgggca ctacgccgtc gtaaatcgcc catgccagcc
    27181 ctgcagcatc catcttatcg gtcactttcg ccaccacgcc gcattgcacc agcgttttat
    27241 cggtgacgat cagcgccttc tgataaccac ggcgtttcac ctcatcggtt aaagccccaa
    27301 cagcaccccg accaaaccat gccgtttcgt tcagaatcat tctgttagcc atcatccttc
    27361 tccttgttgc tttacgaaat tactcttcaa ttcgtaaccc ataggttttg aatttctcca
    27421 gcactacggc aatctcttca tcgctcagca ctggcaccgg gtccgtaatc gccagggtcg
    27481 tcaggtaaag ttgcgccagc acttcaactt catgcgccag ccataacgct ttttccagat
    27541 tcacctcaca agcgataagc ccatgatgtt gtaacaaagt tgccttacga tttttgagag
    27601 cctgcgcaac atgttcagaa agttcacgtg ttccaaaggt cgcataaggc gcgcaaggaa
    27661 tagaattacc gccagccgcc gcaatcatgt agtgaatagc ggggatcgat cggttaagaa
    27721 tggaaactgc cgtgcaatga acggcatgat tgtgaacaac cgcgttggca tccggtctgc
    27781 tttgataggc cgccatatgg aaacgccatt cgcttgaggg gagctttcct tcctcatgtt
    27841 taccgttgcc atcaataaag acaatatgcg actccgtcag tttttcatat ggaatgcctg
    27901 taggcgtaat cagcatccca tcctgataac gtacactgac gttccccgct gtcccctggt
    27961 tcagtcccag gcgggtcatt tccaggcaag tgtcaataat ctgacgagca agtttatttc
    28021 gttccattag ctacctctct ctgattcaaa acagggcaat aatgttgttc ctttcacact
    28081 attgaattag ccgtttaatt acccaccatc ttcttcctga ttaaccagaa agaaattcac
    28141 aagcttatat tttgtgacct ggttcaacta atcacagtaa ataactgcaa gttctctttt
    28201 tataacccca ttaaaaatga ccgctcttaa aaatatttat caaaacggtc atttttctat
    28261 tcactcaagc caggaatgcc cgttttcggc ataaacaatt aatacggtca tctggtttgt
    28321 gttttttatg atttattttc tgaaacgggc atgaaatttc gattattaaa gtgatggtag
    28381 tcacataaag tcaccttcca gctaataagt gtgaccgccg tcatattaca gagcgttttt
    28441 tatttgaaaa tgaatccatg agttcatttc agacaggcaa atattcactg atatgaagcc
    28501 cgaactcgct ggttttgcac ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac
    28561 ctaagaggat gctatgggaa acacatcaat acaaacgcag agttaccgtg cggtagataa
    28621 agatgcaggg caaagcagaa gttacattat tccattcgcg ctgctgtgct cactgttttt
    28681 tctttgggcg gtagccaata accttaacga cattttatta cctcaattcc agcaggcttt
    28741 tacgctgaca aatttccagg ctggcctgat ccaatcggcc ttttactttg gttatttcat
    28801 tatcccaatc cctgctggga tattgatgaa aaaactcagt tataaagcag ggattattac
    28861 cggattattt ttatatgcct tgggcgctgc attattctgg cccgctgcag aaataatgaa
    28921 ctacacctta tttttagttg gcctatttat tattgcagcc ggattaggtt gtctggaaac
    28981 tgccgcaaac ccttttgtta cggtattagg gccggaaagc agtggtcact tccgcttaaa
    29041 tcttgcgcaa acatttaact cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct
    29101 tattttgtct aacgtgccac atcaatcgca agacgttctc gataaaatgt ctccagagca
    29161 attgagtgcg tataaacaca gcctggtatt atcggtacag acaccttata tgatcatcgt
    29221 ggctatcgtg ttactggtcg ccctgctgat catgctgacg aaattcccgg cattgcagag
    29281 tgataatcac agtgacgcca aacaaggatc gttctccgca tcgctttctc gcctggcgcg
    29341 tattcgccac tggcgctggg cagtattagc gcaattctgc tacgttggcg cacaaacggc
    29401 ctgctggagc tatttgattc gctacgctgt agaagaaatt ccaggtatga ctgcaggctt
    29461 tgccgctaac tatttaaccg gaaccatggt gtgcttcttt attggtcgtt tcaccggtac
    29521 ctggctcatc agtcgcttcg caccacacaa agtcctggcc gcctacgcat taatcgctat
    29581 ggcactgtgc ctgatctcag ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt
    29641 atgcagcgcc tttatgtcga ttcagtaccc aacaatcttc tcgctgggca ttaagaatct
    29701 cggccaggac accaaatacg gttcgtcctt catcgttatg accatcattg gcggcggtat
    29761 tgtcactccg gtcatgggtt ttgtcagtga cgcggcgggc aacatcccca ctgctgaact
    29821 gatccccgca ctctgcttcg cggtcatctt tatctttgcc cgtttccgct ctcaaacggc
    29881 aactaactga acatattttc cgaataaagt gaggaatctg taatgaaaaa aatcagctta
    29941 ccgaaaattg gtatccgccc ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt
    30001 gaagaacaaa caatgaatat ggcgaaagct acggccgcac tgctgaccga gaaactgcgc
    30061 catgcctgcg gagctgccgt cgagtgtgtc atttccgata cctgtatcgc gggtatggct
    30121 gaagccgctg cttgcgaaga aaaattcagc agtcagaatg taggcctcac cattacggta
    30181 acgccttgct ggtgctatgg cagtgaaacc atcgacatgg atccaacccg cccgaaagcc
    30241 atttggggct ttaacggcac tgaacgtccc ggcgctgttt acctggcagc ggctctggca
    30301 gctcacagcc agaaaggcat cccggcattc tccatttacg gtcatgacgt tcaggatgcc
    30361 gatgacacat cgattcctgc cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt
    30421 ttggccgtcg ccagcatgaa aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt
    30481 atcgccggtt ccattgttga tcacaacttc tttgaatcct ggctgggaat gaaagtccag
    30541 gcggtggata tgaccgaact gcgtcgccgt atcgatcaga agatttacga cgaagccgaa
    30601 ctggaaatgg cactggcctg ggctgataaa aacttccgct atggcgaaga tgaaaataac
    30661 aaacagtatc aacgcaatgc cgagcaacgc cgcgcagttc tgcgcgaaag tttactgatg
    30721 gcgatgtgta tccgcgacat gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg
    30781 gaagaatcac ttggctacaa cgccatcgct gcgggcttcc aggggcaacg tcactggacc
    30841 gatcaatatc ccaatggtga caccgccgaa gcgatcctta acagttcatt tgactggaat
    30901 ggcgtgcgcg aaccctttgt cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg
    30961 ctaatgggtc accagctcac cggcaccgct caggtatttg ccgatgtgcg tacctactgg
    31021 tcaccagaag caattgagcg tgtaacgggg cataaactcg atggactggc agaacacggc
    31081 atcatccatt tgatcaactc cggttctgct gcgctggacg gttcctgtaa acaacgcgac
    31141 agcgaaggta acccgacgat gaagccgcac tgggaaatct ctcagcaaga ggctgacgct
    31201 tgcctcgccg ctaccgaatg gtgcccggcg atccacgaat acttccgtgg cggcggttac
    31261 tcttcccgct tccttaccga aggcggcgtc ccgttcacca tgacccgtgt caacatcatc
    31321 aaaggcctgg gaccggtact gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat
    31381 gtgcatgaca tcctcaacaa acgcaccaac tcaacctggc caaccacctg gtttgcaccg
    31441 cgcctcaccg gtaaagggcc gtttacggat gtgtactcgg taatggcgaa ctggggcgct
    31501 aaccatgggg ttctgaccat cggccacgtt ggcgcagact ttatcactct cgcctccatg
    31561 ctgcgtatcc cggtatgtat gcacaacgtt gaagagacca aagtgtatcg tccttctgcc
    31621 tgggctgcgc acggcatgga tattgaaggc caggattacc gcgcttgcca gaactacggc
    31681 ccgttgtaca agcgttaata cattactccc tgatgtgatg cccggtcgct ccggctaccg
    31741 ggcctgaaca agcaagagtg gttagccgga taagcaatgt tatccggcta tattgcagga
    31801 gcgattatga aacaagaagt tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc
    31861 atcgcggtta atcggcaggg aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat
    31921 atcgcgatgg aaaacaacac ctggcaccag tggtctttag acgccatttt gcaacgcttt
    31981 gctgattgct gtcggcaaat caatagtgaa ctgactgaat gccacatccg cggtatcgcc
    32041 gtcaccacct ttggtgtgga tggcgctctg gtagataagc aaggcaatct gctctatccg
    32101 atcattagct ggaaatgtcc gcgaacagca gcggttatgg acaatattga acagttgatc
    32161 tccgcacagc ggttgcaggc tatttctggc gtcggagcct ttagtttcaa tacgttatat
    32221 aagttggtgt ggttgaaaga aaatcatcca caactgctgg aacgcgcgca cgcctggctc
    32281 tttatttcgt cgctgattaa ccaccgttta accggcgaat tcactactga tatcacgatg
    32341 gccggaacca gccagatgct ggatatccag caacgcgatt tcagtccgca aattttacaa
    32401 gccaccggta ttccacgccg actcttccct cgtctggtgg aagcgggtga acagattggt
    32461 acgctacaga acagcgccgc agcaatgctc ggcttacccg ttggcatacc ggtgatttcc
    32521 gcaggtcacg atacccagtt cgcccttttt ggcgctggtg ccgaccaaaa tgaacccgtg
    32581 ctctcttccg gtacatggga aattttaatg gtccgtagcg cccaggttga tacttcgctg
    32641 ttaagtcagt acgccggttc cacctgcgaa ctggatagcc aggcagggtt gtataaccca
    32701 ggtatgcaat ggctggcatc cggcgtgctg gaatgggtga gaaaactgtt ctggacggct
    32761 gaaacaccct ggcaaatgtt gattgaagaa gctcgtctga tcgcgcctgg cgcggatggc
    32821 gtaaaaatgc agtgtgattt attgtcgtgt cagaacgctg gctggcaagg agtgacgctt
    32881 aataccacgc gggggcattt ctatcgcgcg gcgctggaag ggttaactgc gcaattacag
    32941 cgcaatctac agatgctgga aaaaatcggg cactttaagg cctctgaatt attgttagtc
    33001 ggtggaggaa gtcgcaacac attgtggaat cagattaaag ccaatatgct tgatattccg
    33061 gtaaaagttc tcgacgatgc cgaaacgacc gtcgcaggag ctgcgctgtt cggttggtat
    33121 ggcgtagggg aattcaacag cccggaagaa gcccgtgcac agattcatta tcagtaccgt
    33181 tatttctacc cgcaaactga acctgaattt atagaggaag tgtgaaatgc tgaaaacaat
    33241 ttcgccgtta atttctcccg aactattgaa agtgctggca gagatgggac atggagatga
    33301 aattattttt tccgatgctc actttcccgc ccattcgatg ggaccgcagg tgatccgcgc
    33361 tgatggcctg ttggtgagcg acttgctcca ggcgattatc ccgttatttg agctggacag
    33421 ttatgccccg ccgctggtga tgatggcagc ggtagaaggt gacactctcg atcctgaagt
    33481 agaacgacgt taccgtaatg cgctttcact acaagccccg tgtcctgaca tcatccgcat
    33541 caatcgtttt gcgttttatg aacgggcgca aaaagccttt gcgatcgtta tcacaggcga
    33601 acgagcgaag tacgggaata ttcttttaaa aaaaggggta acaccgtaat ctcataccgg
    33661 tacgcccgca tgacgcgggc ggttatcgaa tgatggggtg aaaaatatga aagcggcacg
    33721 ccagcaagcg atagtcgacc tgctgctgaa ccataccagc ctgaccacgg aagctctctc
    33781 tgaacagcta aaggtcagta aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca
    33841 gggtaaaatt ctgcgcaatc atggacgcgc taaatatatc caccgtcaaa atcaagacag
    33901 tggcgatccc tttcacatcc ggctgaaaag ccattatgcg cataaagcag atatcgcgcg
    33961 cgaggcgctc gcgtggattg aagaagggat ggtgatagcc ttagacgcca gttcaacttg
    34021 ctggtatctg gcacgccagt tgcctgacat caacattcag gtcttcacca atagccatcc
    34081 gatttgccat gaactcggta aacgcgaacg cattcaactg atcagttccg gcggcacact
    34141 tgagcgcaaa tatggctgtt acgtcaatcc ctcgctgatt tcccaactta aatcgctgga
    34201 aatcgatctg tttatttttt cttgtgaagg gatcgatagc agcggcgcac tgtgggactc
    34261 caatgccatc aacgctgatt acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt
    34321 attgattgat aaaagtaaat ttaatcgttc aggggaagcc cgcatcgggc atctggatga
    34381 ggtaacgcac attatttctg atgagcgcca ggttgcaact tctttggtaa cagcctgacg
    34441 gaaagggtag caggccggag acgacgcccc ggccttgcct gttatcgctc gtcgcgacga
    34501 ccacccaccg cagcccagat gcggcggacg tgcaccgtca cttcttcgcg atcgtgatac
    34561 aactgccgcg cctgaatctg agcatttatg ccatgttcat caagctgtgc ctgaatatac
    34621 gccagattgt gtgatacttc ttcgtagcgt tttttcatcg gcagtttgag attgaaaatg
    34681 gtttcacggc accagccatt aaccagccac tgcgccatca atgccgcaac tttagccggt
    34741 ttttcaacca tatcgcatac catccaggag atattgctgc gtgtcggacg gaatttgaaa
    34801 ccgtcttccc gcagccacgt cacctgcccg gtatccatca gactttgcgc catcgggccg
    34861 ttgtcgacgg aataaaccca catgttgcgc ttcaccagtt ggtaggtcca gccgcctggg
    34921 caagcgccca aatccaccgc ccacatcccg ttcgccaggc gttcatccca ctcatccgca
    34981 ggaataaaca catgaaatgc ctcttccagt ttgagtgtgg aacgactcgg cgcatctgcc
    35041 ggaaatttca ggcgcggaat gcccatatag aacggcgaat tattgttgct gtatgagtaa
    35101 ccggtatagc agcaacctgg tgcaatgaag aatacatgca caaccggacg cttcggcgtt
    35161 tcatagttcg ccagcacccc ggcatcgcgc aaggcagcgc gtagcggcac ggtgaattta
    35221 cggcagaatt tcagcaactc tttgctttcg ttggtatcgg caacttcaac acgcagttca
    35281 ccgcccttct ctactacgcc ctgtaacatg ccgacaatgg gggtaatacg atcttctggc
    35341 ggcaaatgct gcaggagttc ccccaccaca aaccactggc gggcaaaaat taatgaactg
    35401 aacggcagct cacggattaa cttatcgcca tcatcaggtt gataacattc ataaatgaca
    35461 taacccgcat tctctttcac gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg
    35521 gtaatttctg cggcgcactc tttttcaaag cccggacggc acagcaatac aaccttattc
    35581 atgacttacg cccttgcgct ttaaacggat agcaccaact aacattaacg cccagcccgc
    35641 aaggaagctc acgccgccaa ccggagtgac aaacgcccac aaacgcagat gggacagtgc
    35701 caggcaataa aggctgccgc tgaacagcac cgtgcctaac gcgaggaaaa cgctactcca
    35761 gtaaaaccag atactgatgc gacgctgcat tgccaccgcc agacctaaga tcgccagcgt
    35821 atgaaacgcc tggtattcga ggccggtctg gatccagccc atctcaacgg cccccatggt
    35881 tttacttaac acatgcgcgc caaaagcgcc cagagccaca aaaatgaagc cgctaatggc
    35941 ggcgaaaatc agcataaaac ggctggtcat ggtcgtaccc tacgtaaatt attgttcata
    36001 acgaaagcgg aatttttctt gttcagcagc ggctttcgcc aggatccatt ggcgaaaggc
    36061 ggctatttta cccagttctg cctggctgtc atgacaaacc agataaaaag cgtttttact
    36121 gaccagaaca tcattaaacg ggcaaacaag acgtccggct tcaatttcag attgcgccat
    36181 cacgttattt gccagcgcca ctccctgccc gtgaatagcc gcttgcagca ccatggcgct
    36241 atgactaaaa attggccctt gctgaacgtt gatatgattt aaccccaact gtcgggtata
    36301 tgtctgccag tcacggcgtg acgcatcatg taataacgta tgtttagcca gatcttccgg
    36361 ggtcttcaag ggtttttcgc ctgtcagcag tagcggcgaa cacaccggca ataaatattc
    36421 ggcgtacagt ttttccaccc gtagccccgg ccagttgccc cgaccataaa atatcgccac
    36481 atcaacatca tccgccagct tatcttcctg acgatcaacc gcctggattc gaacgtcaat
    36541 tcccggataa gctgaattaa agctggaaag tcgcggaacc aaccaatgaa tggcgaaact
    36601 ggggagtaaa ctgaccgtca acgccccctt ggcgctacgg gcctggagtt tacgcgtcgc
    36661 ttcggttaat tgcgaaaata tctctttgat atcgaggaaa tagctttgcc cttcctcggt
    36721 cagcaggagt gaacgattac ggcggcggaa cagttttagc cccaaaaaat cctcaagaga
    36781 cttgatttga tgacttactg cggcctgggt cacaaaaagc tcttctgctg cgcgagtgaa
    36841 acttaaatgg cgtgctgcgg catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt
    36901 agacatggct tttaaacttt gatgttaaat gaatttaaca attagatcgc actgtgtaac
    36961 ctattagttt ttttaatctg agccattata aattgtccgt tgagcttcta ccagcaaata
    37021 cctatagtgg cggcacttcc tgagccggaa cgaaaagttt tatcggaatg cgtgttctgg
    37081 tgaacttttg gcttacggtt gtgatgttgt gttgttgtgt ttgcaattgg tctgcgattc
    37141 agaccatggt agcaaagcta ccttttttca cttcctgtac atttaccctg tctgtccata
    37201 gtgattaatg tagcaccgcc taattgcggt gctttttttt accttgcgat cgtgaattac
    37261 tgatccagtt cgaccatctc tttcacgtca gtacggttga tctgctgttt gttgccatta
    37321 gcgtctttat acgaaatcat accggtatcg ttatcagtct gtggtttgcc gtcagagacg
    37381 atggtacgtc cgtcattggt gtgcatcaca tagttcgaac cggaacaggc gctcagggca
    37441 aaagtcagca tacaggcaga aataattgcg gcagtctttt tcatcatctt ctccttaaag
    37501 cgattaataa atcttaatag tattgattaa caggctaaaa ttaacgccta acactattca
    37561 gcatatgtta cttacgcggc ttcgccagga tatccagata attctgatgg ttagcactct
    37621 ccttgtatca aagtgaattt tgcgtcacga tcggtgcatc aagccgagga gtaccatgaa
    37681 cgtttttaat cccgcgcagt ttcgcgccca gtttcccgct ctacaggatg cgggcgtcta
    37741 tctcgacagc gccgcgaccg cgcttaaacc tgaagccgtg gttgaagcca cccgacagtt
    37801 ttacagcctg agcgccggaa acgtccatcg cagccagttt gccgaagccc aacgcctgac
    37861 cgcgcgttat gaagctgcac gagagaaagt ggcgcaatta ctgaatgcac cggatgataa
    37921 aactatcgtc tggacgcgcg gcaccactga atccatcaac atggttgcac aatgctatgc
    37981 gcgtccgcgt ctgcaactgg gcgatgagat tattgtcagc gtggcagaac accacgccaa
    38041 cctcgtcccc tggctgatgg tcgcccaaca aactggagcc aaagtggtga aattgccgct
    38101 taatgcgcag cgactgccgg atgtcgattt gttgccagaa ctgattactc cccgtagtcg
    38161 gattctggcg ttgggtcaga tgtcgaacgt tactggcggt tgcccggatc tggcgcgagc
    38221 gattaccttt gctcattcag ccgggatggt ggtgatggtt gatggtgctc agggggcggt
    38281 gcatttcccc gcggatgttc agcaactgaa tattgatttc tatgcttttt caggtcacaa
    38341 actgtatggc ccgacaggta tcggcgtgct gtatggcaaa tcagaactgc tggaagcgat
    38401 gtcgccctgg ctgggcggcg gcaaaatggt tcacgaagtg agttttgacg gcttcacgac
    38461 tcaatctgcg ccgtggaaac tggaagcagg aacgccaaat gtggctggtg tcataggatt
    38521 aagcgcggcg ctggaatggc tggcagatta cgatatcaac caggccgaaa actggagccg
    38581 tagcttagca acactggcgg aagatgcgct ggcgaaacgt ccaggtttcc gttcattccg
    38641 ctgccaggat tccagcctgc tggcctttga ttttgctggc gttcatcaca gcgatatggt
    38701 gacgctgctg gcggagtacg gtattgcctt gcgggccggg caacattgcg ctcagccgct
    38761 actggcagaa ttaggcgtaa ccggcacact gcgcgcctct tttgcgccat ataatacaaa
    38821 gagtgatgtg gatgcgctgg tgaatgccgt tgaccgcgcg ctggaattat tggtggatta
    38881 atgacgaacc cgcaattcgc cggacatccg ttcggcacaa ccgtaaccgc agaaacgtta
    38941 cgcaatacct tcgcaccgtt gacgcaatgg gaagataaat atcgccagtt gatcatgctg
    39001 gggaaacagc ttccggcttt gccagacgag ttaaaagcgc aggctaaaga gattgccgga
    39061 tgcgaaaacc gcgtctggct gggatatacc gtggctgaaa acggtaaaat gcacttcttt
    39121 ggcgacagcg aaggacgtat agtgcgcggc ctgctggcgg tgctgttgac tgccgttgag
    39181 gggaaaaccg ccgccgaatt gcaggcacag tcgccgctgg cattgtttga tgagctggga
    39241 ttgcgtgggc agcttagcgc ctcacgcagc caggggttga atgcgttaag cgaggcgatt
    39301 atcgctgcga cgaagcaggt ttaagcacag aaccaggtcg gataagacgt tcgcgtcgca
    39361 tccgacctga ttgtttaacc ctgacgcgcc gctttcgcca tcatcttctt cagcgcatga
    39421 gaaaccgcaa caaacccaaa ggtggcggtc accatcgttg ccgcgccaaa tccagatgca
    39481 caatccatcc gcttcggccc ttctgccgtg gctttcatcg cacacacagt accgtctgac
    39541 tgcgggtaca ccagcgcttc agtagaaaac acgcaatcga cgccgagctt acctttactg
    39601 tttttcacta cgccaaaatc gcttttcagg cgctcgcgca acttcgccgc cagcggatcc
    39661 tgaatcgttt tcgccagatc ggtaacctga atctgcgtcg ggtcaatctg cccacccgcg
    39721 ccaccggtcg tgaccagcgg gattttattg cgccgacaat aagcaatcag cgccgctttg
    39781 ggccgtacac tatcaatggc atcaatcacg tacgaatacc ccacgctcat atactgcgct
    39841 acgttatccg gcgtcacgaa atcatccacc accgttacac ggcactccgg gttaatctgg
    39901 cgaatacgct ccgccatgac ttccgctttt gccagcccga cgttatcgcg cagagcgtga
    39961 atttgccgat tggtattggt gacgcacaca tcatccatat cgataagcgt gattgcgcca
    40021 atccccgtgc gcgccagcgc ttccgccgcc caggaaccga caccaccgat accaaccaca
    40081 caaatatgcg cgtcagcaaa cagttgcaac gctttttcac catacagacg cgctgtgcca
    40141 ccaaaacgct gacgccatgc atcactaatt accacagaca tagcacctca gaattaaaaa
    40201 gggtgaggtt atccctcacc ctgtcgtatc cgtaaaaacg gtatacagaa taacacatca
    40261 gccgctaaag acgttacctg cgcccggggc ggttttcagc acccagacac gtccatagtg
    40321 gttgtaccaa cctgcgcgat gtccggcttc cggcccgatc ccttgataga tatcgaagtg
    40381 ttggcctttg attgcgccac cgacatccag cgccaccatc agacgcaatt cgtactgacc
    40441 gttaaatttg ccgttattat ccagcagcgg cacttctgcc agcaaggtag tacctggcgg
    40501 aataattgaa cgatcggagg caacagaagc acggccaacc agcggcaccg cacttgcccc
    40561 tttcaccggt gcaaaagatt gcggtttaaa gaaaacgaaa gacgggttct gttccagcag
    40621 ctcgcgaacc tcggcttcac tgtgtgtttc gccccaatga cgaatcgcct gcatcgacat
    40681 atcttctttt ttcacttcgc cacggtcgat cagcacctta ccaatgctgc gataggcatg
    40741 accgtttttc cctgcatagc tgaaaaagtt aagcggactg ccatcaccaa agtcgatata
    40801 cccgctaccc tgcacatcca taatgaagtt atccatcagg gagttgctgt aagcgagaat
    40861 atatttatca ctcagcgccc ctgcgtagat ctccgcacga gacggcagac gaccacgttt
    40921 tggcggcata cggtaaatag gatactggaa ctcgccctgg cgggtatggc gcgcctgaat
    40981 taccggcgtg taatagcctg taaactgcac gttaccatag ttgtcggcac cttccatctg
    41041 ccaggcatca atgccgaact ggcgcatatt gcgggtatca ccgcctgcgc gcagccactc
    41101 ttgcaccgcg ttataaacat tactctggtt gccatacaga cgcggtgacg aattacggat
    41161 atggttaatt tgctcggcaa aatcaccggc gttaatcggc gcgccaacgg catctggctg
    41221 gttcaccaga gagaaaggct gggtaaattt cccgtcttta tattgctgtc cgcgatcggt
    41281 tggtttggaa gagcaggcgg caagcattgc cacaaccgtg cccataagaa ggtactttac
    41341 ccaacgtcct ttcattgttc tcttcttcag gttaaaaaat aaggcgcaac gaagataaca
    41401 aaccgccgcg tttaatgaaa tgagcagcct ctccctgacg caaattttgc acaaaaaata
    41461 ggctttagtg gtttgttttt gttcaaaatc atgctaaatc cgtgatcggg gtaaaaaaaa
    41521 ggttgcatga aaacgcgagc ggagtatagt gcgcatccac ggacgcgggg tggagcagcc
    41581 tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca
    41641 atacttagaa tgctgataaa ccgcaatgtg ttccacaaat tgcggttttt tttgtattta
    41701 aaaacttcgt tcgctgaact cacaacacgt tttttatctc ccttcctgcg aagtaatcaa
    41761 ttatcgactg ggcaacgtgt gtaccggttt gctggtatga ctcaacggtg gcggccgcaa
    41821 tatgcggcgt tgtaatgata tttttggcat gtagcagtgg gctaaattgc gccaacggtt
    41881 cagagctaaa cacatccagc gccgcaaaca catttcgttc ttcaatcacc tgtagtaaag
    41941 cgccctcatc cagcaacttt ccccttgcgg cattgatgat tagagcacca ttgcgcatca
    42001 tcgacaaata tttatgacta atgaagttct ccagctcagg agaaaaaggc aaaagaacaa
    42061 tgatgacatg cgactgcata aagatatctt caattgatac aaactgaact ccaactttgt
    42121 cagcaacctg ataatcgggt tgtttgtcat aagctaaaag ctcaacatca aaaccggata
    42181 acctttttgc cagggtttta cctatattgc cgtagccaac catgccaatc cgtttgcctt
    42241 gtaattcata gccatgcgac tcaccccagt agccattttg catcgcatga tagctgcctg
    42301 gaatatttct catgctcgcg aagataagtc caatgataaa ttccgcgaca gcatttgaat
    42361 taatcccaac cgcattattg actacaatac cggattgttg tgctgcacga agatcaatat
    42421 tatctatccc cgtcccgaat ctcgatatta ttttcacacc aggcagcaat gatagcgtgt
    42481 ccgcctgata acaatctttt ccggcgatga ccgcgcaaat agattctcgt aatgcagtgg
    42541 attctgattt aagcagatgc tccacattat tcttaataat aagattaaaa ttattctctc
    42601 ttagcaactt gctggcggaa acacagcgtg ctgaaaagct ggggacggtg accagaacat
    42661 ttttcatagc tgacactcac ttcaatgaac gccaccccaa aagatggcgt gaccggttac
    42721 tgtatttata ctttgaaatt atgaatttgc tttaatgccg agtgcacctc ctggatgata
    42781 taagccgaaa tcagcacgag taaatttttt caattcagag agtgcaagcg ccaccgcatc
    42841 accaacaacc agaacaacag tagacgagca tgacggtgct aaacctaaat tatccgcctc
    42901 acttttgacg gggatttcaa cagataaatc acaacttatc gcaagcgatg agtgatgact
    42961 gcgggtaaaa gagatcaaat aattacccat ttttttcaaa ctaggcagtg tcgccaggat
    43021 ttccgccgtc tcaccactgt ttgaaataag aataacaaca tcgtctttcg ccaccatccc
    43081 aaggtcgccg tgtaccgctt ccgtaccgtg aacaaaaaac gagggagttc cggtgctggc
    43141 aaatgttgca gcaagttttc tggcaataat gccagatttc ccaacaccaa taaaaacaac
    43201 ttttccctgg caagattgta atattttaat taaattcgcg acttccgaaa ggggggaatc
    43261 aataacctct tctactgctt tatgttcgtt acgcataaaa cgtttaataa gatggataag
    43321 atccgtattg ttcattttct ttttcctgat taatattttt aattacactc gcaaggcgcg
    43381 caagccccat atataatttt tctcgactgc aggccacatt aagacgcaga tagccctctc
    43441 cttcagtacc ataaacatgc cccgccataa tacctactct cccttcgtta attaatcgac
    43501 ggtaaaattt atcaaaggga taaccaaggg ccgagcaatc aacccacgaa aaatagcagc
    43561 cctcagctgg ccatacgatc aattccggaa tattttcatt taaatactgc aaggtaaatt
    43621 gaatgttaga ggcaatatgt acctttaacg actttaacca ttcttcacat tcattatagg
    43681 caacaatggt cgcaattaca ttcagtactg atggggagtt tacgaaatcg cgatacctcg
    43741 atatcgttct gaacttatga ttaaaattat cgtcagttga aatcatatat gatccggtta
    43801 gcgcaggaac attaaatgtt ttcgaaatcg aattaaacac caccactttg tcatctttta
    43861 ttgttagcgc tgacgtgaaa ctatgattaa aaacaaaatc actatgtatt tcatctgaac
    43921 ataaccaaat attatgcctt gcacagcatg ctgcgattct gtgaagatcg tcttccgacc
    43981 agaccgttcc tgtcgggtta tgaggattac ataataacac catcttcgcg cttttaattt
    44041 taagctcaaa gtcttgccag ttgataacgt agcctctccc attttctttc atcaaagagg
    44101 ttgttagcat gtttcgacca ttatgcttaa tcacatcaaa aaatgcgtta taaacaggcg
    44161 tgaaaatgag aatattatct ccattcaatg tcaatatttc tattgcctta gatattgaat
    44221 acatcacgct tgggctatag gtgatccatt ctggttgaat ctctgtttta tattgttgat
    44281 gataccaatt gactatcgaa ttcagatagt catcatgctg ccaacgactg taccctaaaa
    44341 tcgggtgttc cagacgtttt tttaatgttc tgatgatagc ttccggtatc ggaagatcca
    44401 tatctgaaat agagaatggt aatacatctt ttacaccaaa tctgtcttca caaaagtccc
    44461 attgtgtgca atatgttcct ttacgagaaa ttgaggtatt aaaaatatcc atcaacatat
    44521 ccccatgtaa ttatccatat cccgacgtag ggcgatgact tttgtaccga taatgacttg
    44581 taaggtattt tgatcaagtt tcacaaccgc aattcctccg gcccttttta ttgcagcctc
    44641 atctatcaat cccatatccg cgatagttaa acgcaaacga gttacacaat tatccagtga
    44701 tttaatattt tctttaccac ccagagcctt aaccatttgc tccgctgcct gcaactctga
    44761 tttatcatga actgggatgt tagctattgc agtttcttcg tgagcgttca gcgtgacagg
    44821 actggactct tcacggccta cagtcatcag gttaaatttg agaatggtcc aacggaacac
    44881 aaagtaataa atgaagaaat aagccacttc aacacatacc gctaatggcc atcccgttct
    44941 tgtgccacgt aagacaccaa agctaataaa ttgaatcaga tcgccagtaa agcccatctt
    45001 cacgcccata tatggcagaa ccatgttagc caatccattc ataaaggcat ggaagacaaa
    45061 caacagcggg ctgacaaata agaacagaaa ttcaatcggc tcgctgacgc cgccaataat
    45121 taccgtgagc acaccggaaa tcagcaatgc tttgatagcc cttctgtttt gagcatgagc
    45181 agtgcgatac atcgccagcg cggctccagc cagaccatac tgaatcatca gtttgccctg
    45241 ctccattttc cccgccaaat ccagcggaat gaccgcattc tctttgacgt atgccataaa
    45301 catgttcagg gtgccgtaat actcttcacc gccaatcact gccgatccgc cgattggggt
    45361 aaagcggaaa actgatgtca ccaagtgatt taagccaaaa ggtatcgtca cgcgttcggc
    45421 aacagcatag aagaaataac caacaggacc ggaagttgaa atccagtgac caatgagcat
    45481 gaaaagattg aaaaatggcg gccagataaa aggaatgaac agaccaaatc cactcatcac
    45541 aatcagtgta atgataggca caaggcgtgg gccactataa aaccctaatg attcaggaat
    45601 acgtaagtta acgatctttt tatacatgct ggcgactaat aaccctgcca caatcccacc
    45661 caacacgctg gtattgtagg actggatccc gagtatgatg gtttgcccat gtgtcgacat
    45721 ttggtcagca gctaccaata agtcgtgctg tttaagataa aagttcgttc caagatgcat
    45781 cgccataaag ccaattaagc cagaaaaagc gccataggct ttatcatctt tctcttttaa
    45841 taatcccaaa ggtatagcta tcgcaaacaa tacaggtaaa ttaacgaagg caaacaaacc
    45901 aaggctaaca ataaaatcaa gtatggtttt aattaacgga atagccaaca ctggaatgaa
    45961 ttttcccata tcatcgctgg ctaaaccact tcccagccct aacatcatac cacatacact
    46021 taacagagcg atgggataca taaatgcctt ccccaggctc tgaaaaaaac tccaggcttt
    46081 cttttgtttc atactggtta tctcatgaaa atgttatgta taattagtcc attaatactt
    46141 tgatgcgaat agaaagatat agtttctctt ccagaagaga atcatattta aaagttgcgt
    46201 acagatatct ttcaatttca tgaatggcac gttgtaactg aaaatctttt tcaagaaact
    46261 gctcatagat atcaccaata tttttctgtt ttattttttc attacataac acacgactag
    46321 aaaaatattt aaggtgcatc ataaatctgg agtagttaaa tgaacttctg tccacccttc
    46381 tgttataaac aacttcaata agctccgtga ttttttgaga cagctcaata atccttactg
    46441 attcataatc aactgaattt tcataattat ttaaaatatg caatgctata aaaccaatct
    46501 catcatccgg tagttgaacg ctaaattgct cattaatgat tgttaatgca tcacgactta
    46561 ttgcatactc tgctttgtac aacgctttga tttcctcaag gaaaacatta ttaatttgta
    46621 tgcctgagac gactcgggat attgcattcg aaatgtgatc agcaagaatc agtagtagcg
    46681 atggattgag ttttttttcg agattactct ccgcataatg cactatcttt tcggcaacaa
    46741 aaatatactc aatacccaca tgctcaatca atttgtagag tttatttttt tgctcatttc
    46801 tgacataaaa aatccggttt gcaggatgct cagggaccga cataccatat tttttattgt
    46861 aaccaacgcc aggcccagaa atgataacct cctgtccgtt caccgatgcc tgtacacaat
    46921 tattgttcat gactttttcg atgatcattc catggctccc aaaaaaaaag caaggcatgt
    46981 cagaagtaat aattacttca aacattgcct tgcctgattt aacagttaca agataattaa
    47041 ctaatgcgta taccttacac ggaattgtgc ttaataaaag agatgacagg cttatttttt
    47101 aagccgatat tcaaatattc gacagcagtc acaaaaccat cgttcaaca
//
LOCUS       DAZFKM010000022        46761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFKM010000022 DAZFKM010000000
VERSION     DAZFKM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2132)
                     /locus_tag="ACHKAY_001363"
     CDS             complement(<1..2132)
                     /locus_tag="ACHKAY_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5700070.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQESDRQKGKKSWQAERYARGNLRKIFERLERTDHRWL
                     TPGYRSLTGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDSFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDSIVAITRDIAIQEDRHELGNDITPRFKVEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEPEDNAHENGFDLQGGP
                     AASWTRGNNCPRVQETDNNGTKQPEERPA"
     gene            complement(2129..2518)
                     /locus_tag="ACHKAY_001364"
     CDS             complement(2129..2518)
                     /locus_tag="ACHKAY_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO5700071.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPILRGLFDLYIRGRDNRARTTKPEN
                     VDTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCVS
                     LSEYTAEFNRLSTIFLQEWQRVTGYQP"
     gene            complement(2842..3045)
                     /locus_tag="ACHKAY_001365"
     CDS             complement(2842..3045)
                     /locus_tag="ACHKAY_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700072.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQDPRK"
     gene            complement(3133..3246)
                     /locus_tag="ACHKAY_001366"
     CDS             complement(3133..3246)
                     /locus_tag="ACHKAY_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700073.1"
                     /translation="MSIFISWLVLIISVVCAIGIMQIIHSVKKIERIFTGE"
     gene            complement(3243..3485)
                     /locus_tag="ACHKAY_001367"
     CDS             complement(3243..3485)
                     /locus_tag="ACHKAY_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5700074.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(3497..3775)
                     /locus_tag="ACHKAY_001368"
     CDS             complement(3497..3775)
                     /locus_tag="ACHKAY_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700075.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHGIISEAEKARKINGDEPYIEIICMDSEAEIQKAD"
     gene            complement(3786..4124)
                     /locus_tag="ACHKAY_001369"
     CDS             complement(3786..4124)
                     /locus_tag="ACHKAY_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5700076.1"
                     /translation="MLKQRRNFRTETERQANRFATSASRSNIRYSLSETHATPDGHTV
                     KQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDTQFGQDFTLYEALRTVDRLLR
                     GQSFIKQTDL"
     gene            complement(4139..4417)
                     /locus_tag="ACHKAY_001370"
     CDS             complement(4139..4417)
                     /locus_tag="ACHKAY_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5700077.1"
                     /translation="MTKLLNTYEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     TVNAVKRQADRAALPINQEEKNSKRTVNLFAIFLKTIRNAEKYVQMTK"
     gene            4509..4820
                     /locus_tag="ACHKAY_001371"
     CDS             4509..4820
                     /locus_tag="ACHKAY_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5700078.1"
                     /translation="MSSGYEKKLKEIRKSEGLTQAEFADVTGINLGTIKNYESGKREV
                     GLSVVDRVINSKDFEKYTIWLMTGKTNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            4909..5844
                     /locus_tag="ACHKAY_001372"
     CDS             4909..5844
                     /locus_tag="ACHKAY_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5700079.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYPLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPIAG"
     gene            5855..6250
                     /locus_tag="ACHKAY_001373"
     CDS             5855..6250
                     /locus_tag="ACHKAY_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700080.1"
                     /translation="MEKTVNSDLHLGQYWRIDIVCDPELRDEVEQYFSLHDVGFSKIE
                     VFSVENPYKLALFFDFAKKGVEVAKAIMGLLDRNDIEITMYRATDSSPQSVKSIKLRK
                     SEDVEKCKDLLSTCVAIGVQRNKEKEGSE"
     gene            6440..7420
                     /gene="btuC"
                     /locus_tag="ACHKAY_001374"
     CDS             6440..7420
                     /gene="btuC"
                     /locus_tag="ACHKAY_001374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO5700081.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            7483..8034
                     /gene="btuE"
                     /locus_tag="ACHKAY_001375"
     CDS             7483..8034
                     /gene="btuE"
                     /locus_tag="ACHKAY_001375"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO5700082.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            8034..8783
                     /gene="btuD"
                     /locus_tag="ACHKAY_001376"
     CDS             8034..8783
                     /gene="btuD"
                     /locus_tag="ACHKAY_001376"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO5700083.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            8861..9325
                     /gene="nlpC"
                     /locus_tag="ACHKAY_001377"
     CDS             8861..9325
                     /gene="nlpC"
                     /locus_tag="ACHKAY_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5700084.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            9572..10285
                     /gene="ydiV"
                     /locus_tag="ACHKAY_001378"
     CDS             9572..10285
                     /gene="ydiV"
                     /locus_tag="ACHKAY_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO5700085.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            10348..11784
                     /gene="selO"
                     /locus_tag="ACHKAY_001379"
     CDS             10348..11784
                     /gene="selO"
                     /locus_tag="ACHKAY_001379"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO5700086.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(11788..11979)
                     /gene="hemP"
                     /locus_tag="ACHKAY_001380"
     CDS             complement(11788..11979)
                     /gene="hemP"
                     /locus_tag="ACHKAY_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO5700087.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(12111..13157)
                     /gene="aroH"
                     /locus_tag="ACHKAY_001381"
     CDS             complement(12111..13157)
                     /gene="aroH"
                     /locus_tag="ACHKAY_001381"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO5700088.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(13314..14147)
                     /gene="ppsR"
                     /locus_tag="ACHKAY_001382"
     CDS             complement(13314..14147)
                     /gene="ppsR"
                     /locus_tag="ACHKAY_001382"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO5700089.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            14480..16858
                     /gene="ppsA"
                     /locus_tag="ACHKAY_001383"
     CDS             14480..16858
                     /gene="ppsA"
                     /locus_tag="ACHKAY_001383"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO5700090.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(16915..18561)
                     /gene="fadK"
                     /locus_tag="ACHKAY_001384"
     CDS             complement(16915..18561)
                     /gene="fadK"
                     /locus_tag="ACHKAY_001384"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO5700091.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(18618..18911)
                     /gene="ydiT"
                     /locus_tag="ACHKAY_001385"
     CDS             complement(18618..18911)
                     /gene="ydiT"
                     /locus_tag="ACHKAY_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5700092.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(18908..20197)
                     /gene="ydiS"
                     /locus_tag="ACHKAY_001386"
     CDS             complement(18908..20197)
                     /gene="ydiS"
                     /locus_tag="ACHKAY_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5700093.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(20253..21191)
                     /gene="ydiR"
                     /locus_tag="ACHKAY_001387"
     CDS             complement(20253..21191)
                     /gene="ydiR"
                     /locus_tag="ACHKAY_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO5700094.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(21211..21975)
                     /gene="ydiQ"
                     /locus_tag="ACHKAY_001388"
     CDS             complement(21211..21975)
                     /gene="ydiQ"
                     /locus_tag="ACHKAY_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO5700095.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLTKALN"
     gene            22291..23202
                     /gene="ydiP"
                     /locus_tag="ACHKAY_001389"
     CDS             22291..23202
                     /gene="ydiP"
                     /locus_tag="ACHKAY_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5700096.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(23245..24396)
                     /gene="ydiO"
                     /locus_tag="ACHKAY_001390"
     CDS             complement(23245..24396)
                     /gene="ydiO"
                     /locus_tag="ACHKAY_001390"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO5700097.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITSAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(24410..26004)
                     /gene="ydiF"
                     /locus_tag="ACHKAY_001391"
                     /pseudo
     CDS             complement(24410..26004)
                     /gene="ydiF"
                     /locus_tag="ACHKAY_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(26148..26906)
                     /gene="aroD"
                     /locus_tag="ACHKAY_001392"
     CDS             complement(26148..26906)
                     /gene="aroD"
                     /locus_tag="ACHKAY_001392"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO5700098.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(26937..27803)
                     /gene="ydiB"
                     /locus_tag="ACHKAY_001393"
     CDS             complement(26937..27803)
                     /gene="ydiB"
                     /locus_tag="ACHKAY_001393"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO5700099.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(27815..29080)
                     /gene="ydiN"
                     /locus_tag="ACHKAY_001394"
     CDS             complement(27815..29080)
                     /gene="ydiN"
                     /locus_tag="ACHKAY_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700100.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(29307..30521)
                     /gene="ydiM"
                     /locus_tag="ACHKAY_001395"
     CDS             complement(29307..30521)
                     /gene="ydiM"
                     /locus_tag="ACHKAY_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700101.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(30621..30977)
                     /gene="ydiL"
                     /locus_tag="ACHKAY_001396"
     CDS             complement(30621..30977)
                     /gene="ydiL"
                     /locus_tag="ACHKAY_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO5700102.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(31113..31220)
                     /gene="rprA"
                     /locus_tag="ACHKAY_001397"
     ncRNA           complement(31113..31220)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKAY_001397"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(31406..32518)
                     /gene="ydiK"
                     /locus_tag="ACHKAY_001398"
     CDS             complement(31406..32518)
                     /gene="ydiK"
                     /locus_tag="ACHKAY_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO5700103.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            32907..35963
                     /gene="ydiJ"
                     /locus_tag="ACHKAY_001399"
     CDS             32907..35963
                     /gene="ydiJ"
                     /locus_tag="ACHKAY_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO5700104.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            35960..36370
                     /gene="menI"
                     /locus_tag="ACHKAY_001400"
     CDS             35960..36370
                     /gene="menI"
                     /locus_tag="ACHKAY_001400"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO5700105.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            36470..36658
                     /gene="ydiH"
                     /locus_tag="ACHKAY_001401"
     CDS             36470..36658
                     /gene="ydiH"
                     /locus_tag="ACHKAY_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO5700106.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            37206..37574
                     /gene="sufA"
                     /locus_tag="ACHKAY_001402"
     CDS             37206..37574
                     /gene="sufA"
                     /locus_tag="ACHKAY_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO5700107.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            37583..39070
                     /gene="sufB"
                     /locus_tag="ACHKAY_001403"
     CDS             37583..39070
                     /gene="sufB"
                     /locus_tag="ACHKAY_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO5700108.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            39080..39826
                     /gene="sufC"
                     /locus_tag="ACHKAY_001404"
     CDS             39080..39826
                     /gene="sufC"
                     /locus_tag="ACHKAY_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO5700109.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            39801..41072
                     /gene="sufD"
                     /locus_tag="ACHKAY_001405"
     CDS             39801..41072
                     /gene="sufD"
                     /locus_tag="ACHKAY_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO5700110.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            41069..42289
                     /gene="sufS"
                     /locus_tag="ACHKAY_001406"
     CDS             41069..42289
                     /gene="sufS"
                     /locus_tag="ACHKAY_001406"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO5700111.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            42302..42718
                     /gene="sufE"
                     /locus_tag="ACHKAY_001407"
     CDS             42302..42718
                     /gene="sufE"
                     /locus_tag="ACHKAY_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO5700112.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            42867..43871
                     /gene="ldtE"
                     /locus_tag="ACHKAY_001408"
     CDS             42867..43871
                     /gene="ldtE"
                     /locus_tag="ACHKAY_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO5700113.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(43935..44171)
                     /gene="lpp"
                     /locus_tag="ACHKAY_001409"
     CDS             complement(43935..44171)
                     /gene="lpp"
                     /locus_tag="ACHKAY_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO5700114.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(44482..45894)
                     /gene="pykF"
                     /locus_tag="ACHKAY_001410"
     CDS             complement(44482..45894)
                     /gene="pykF"
                     /locus_tag="ACHKAY_001410"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO5700115.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(45902..46096)
                     /locus_tag="ACHKAY_001411"
     CDS             complement(45902..46096)
                     /locus_tag="ACHKAY_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700116.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            46451..46660
                     /gene="fumD"
                     /locus_tag="ACHKAY_001412"
     CDS             46451..46660
                     /gene="fumD"
                     /locus_tag="ACHKAY_001412"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO5700117.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT        11658 a        11298 c        12000 g        11805 t
ORIGIN      
        1 gctggccgtt cttccggctg ttttgtcccg ttgttgtccg tttcctgtac acggggacag
       61 ttattgccac gagtccaaga ggcggcaggg ccgccctgaa ggtcaaaacc attttcgtgg
      121 gcgttgtctt ccggttctgg tttacgtctt accagcttcc agttatccgg atgcgtgcac
      181 acacgggagg attccccgat gagtggtgac cagatcccgt aaatctgtac gctctgttcg
      241 ccgtaatcgt tcagctcatc tgcgaggtcg taggcggtgc gaatcaggta gtccttgcgt
      301 ggaacaagca cgccaccctg tttttcaatg taggtggcaa aacatccggc atcagcggca
      361 gcgagtaccg catccattgc gtcatccttc agccgttgcg ggccttccgg gttgcgtgcc
      421 atctggctgg caaggcggcg cagttcacgc cacacctgac gggaggggat accaaagaac
      481 tggaactggc gaacccggtg aaggcgcgcc cagccgatgg cgcgttccac gctctcggcc
      541 attgattttc cggtttcgtg gtcaacgcgt ggcttgcccg ttttcgggtc gatgccatcc
      601 acggcgcggc tgtccaggtt ttttccgatg taggtcgcga tatagctggt tggtgtgcct
      661 tttgagccgt cgacatactc caccttaaag cgcggagtaa tatcattgcc cagctcgtgg
      721 cggtcttcct gaatggcaat atcgcgggtg atggccacga tgctgtcgat ttcttccgga
      781 tgagcaaaga ccatcatatg ccagtgcacg gtgccgtcat ggtgaggctc caccgtgcgg
      841 atgccatacc agcgcagacc gtcgcggttc agttttttgc ggactgccgc aaaaaacgtg
      901 ttaaccaggt aatcgctgga gtcgcgcatg gtggccccgt tccatttggg attcggatga
      961 ccgttctctg ttgtggcgtg gtattttgac gggcaggtga cagtcagaaa caccgctctg
     1021 tcgccacggg cttcggccag aagttccagc cccttcatgg tggccatcat ttctgcctta
     1081 cggtgaaccg ggttacttac tcccgcgtaa tacactgtct cgagatcaat cgtgaacccg
     1141 tcttcgtttt ccagcatgaa acttttcagg aaatcgcgtg ttttctcgcg ctgcgcgcga
     1201 aactcgctta atgcgtcctg actcagatag ggcgatgttt ttctggaaac cagacaggcg
     1261 gcgcggagtt gttcttcccg ccactcgcaa cgtaacagcc acagtttgcg tttccaccat
     1321 tccgcacagg tcaggcgaag gattgcgccc ggcagcagct ccgtgtccgg ttcgttcctc
     1381 cggtctttgt ctgttgtcag tgcgtcataa tgtggaggca tggcgtgcag gtgtaacgcc
     1441 atgcgggcca gcatctgata cgccttcagc gttacatcca tggtcagctc gccatcagtc
     1501 gcgccaaagc tatcgcagag tttttcgaag gtgctgctga acatcgccgc cgtcatggtg
     1561 gccagcgtct gtatctggtg tttgttgagc tgcggcaggt aaagcaaatc gtccaggcgt
     1621 tcgcgtccgg taagggagcg ataacccggt gtcagccagc ggtgatcggt gcgttccagg
     1681 cgttcgaata ttttgcgcag gtttccgcgc gcgtagcgtt cagcctgcca gctctttttg
     1741 cctttctgac gatcggattc ctgttttttg cgcaggaaag agaggtggcg gctcagaggt
     1801 tcacgcagat aaacgggaag cactttcagt gtggcaaaag cacgggccac cgggtcttgt
     1861 tctgttgcct gacgcttgct gatgatgctt tgtgccagct tttcacgctg tccggcttcc
     1921 tcaagggatg ccatgagttt tttacccacg gtggattgtg cgaaaaaggc ttcctccttc
     1981 gcttcctgtt cttccagggc ctttttgtct gcctcaaggt agtaacggat ggcgcgttgc
     2041 aggtcggttt cagtttcctg cctgtgctcc gtaaatctgg ccggatcaat ggctggtcgt
     2101 ggttcattcc agctccatgc aaactcactc atggctggta tcccgtcacg cgctgccact
     2161 cctgcaagaa gatggtggaa aggcggttaa attcagcggt gtattcactc agcgagacac
     2221 acccgccagc agtgcgatgc gccagcattg ccgcaaatac ggaggccggg gagtcgtaat
     2281 acgccagcag tgattcgccg tgcggtgtca ggcagtgcaa cgccagtccg tgtggtgtga
     2341 agtccacgcg gtagcagtcg tctactgtga aataaagggt atccacattc tccggttttg
     2401 tggtgcgtgc tctgttgtca cgaccacgaa tgtagagatc aaataatccc cgaagaatgg
     2461 gagccagacg ggtgtcctgt gtgcgcaccc atcttgtgaa gtcatgagcg tcaatcatgc
     2521 tgcaattctc tctactaagg atgtgcgaag gcttcctgcc aaaaaactca gaagcggtcg
     2581 gttacagaaa ttggcgttct acgagtagtt attgctggct tgaggttcat ttctgtttaa
     2641 ttccgtgcat gctgtagaag aaatcaaaag tgttatctat gcgtatcatg agctcacgct
     2701 gcatcgcttc tggtgtttct ggttcaccta gtgagccaac tcccgcgaag aaatcaccga
     2761 tctcgctttg aataagtgcc tttagcgttg ctgaggaatt caggtgtgtg cggtgatgtc
     2821 tgcgagtgat tcttctcctg ctcatttgcg aggatcctgt acctgtcgga taagattcac
     2881 ccgcgccaca ttagtggcgc agaagtaagt accgtcagtg agatagatgt ggtgtgcatc
     2941 cttttctgag cggtgtttgt cgattgtggt aatcaggcgt tcgtcgactt cgtattcacg
     3001 tcctctggaa gtgaaacgaa cgacaggaaa atgcttaatt gccattacgc ctcctgggcg
     3061 tgtgcgaata cctccgcgaa tgcggattgt ttttacattt tcttatttaa cctagggttt
     3121 ttatttgcgc tgttattcgc cagtgaaaat gcgttcaatc ttttttactg aatgaataat
     3181 ttgcataatc ccaatggcgc agaccaccga aataatcagg acaagccatg agataaatat
     3241 actcatgcaa tattccccag cttatacggt tcaatatgct ccccgcattc tgcggcacag
     3301 atcagctcgg aaagttcgtt aagtgcatcc agatcatcag cgtaaaaagc cacgtcatac
     3361 agactccgga ttgccctggt caatgagtca cgggctgcac gttcagcatg agcacctgat
     3421 gcacttaagc gaaaataaaa acgctcaagt gctttgttaa tgagagtttt atattctttg
     3481 cccatcgcaa cgccctttaa tctgctttct gaatttcagc ttctgaatcc atacaaataa
     3541 tttcgatata gggttcatcg ccattaatct tacgtgcttt ttcagcttcg ctaatgattc
     3601 cgtgtacagt ctggtacgga agttctacgg tcaggcgcgt gccgttcaga taaacgtaag
     3661 tagctgcatt tttttctgat gggacaactc catcaatagc tgatgcgcgc aataacagtt
     3721 caccgcgaaa atcaataaaa cggataaata caccttgtgc atgctctttg gtcataacgc
     3781 acctgttata aatcagtctg tttaataaaa ctttgcccgc gaagcagacg atcaaccgtg
     3841 cgaagtgctt cgtacaatgt gaaatcctgt ccaaactgag tgtcgccgtt gctcagagca
     3901 aaaatgcggt ttccggtaaa tgggttgcgt tggcatctgt gaaccacgat tccagctttt
     3961 tcaatcagcc aggtgtgttc gccaatttgt tttactgtat ggccatctgg cgttgcgtgc
     4021 gtctcgctca ggctgtagcg gatgttgctg cgtgatgcgc tggtagcgaa acggttagct
     4081 tggcgttccg tttcggtgcg aaaattacgg cgttgcttca gcataaaatg acacctcgtt
     4141 attttgtcat ctgcacatat ttctctgcgt ttctgatggt tttcaggaaa attgcgaaga
     4201 gatttactgt acgttttgag tttttttctt cttggttgat gggaagggct gctctgtcag
     4261 cctgcctttt cactgcatta acagtttgat tggtacgctt cgcgtaatct ttcaggcttt
     4321 cttcgagtac cggtaatcca tgctcatcgc ggtatgggta gaacgccgcc aaacgctcaa
     4381 aatccgcttg ctcgtatgtg ttcaagagct ttgtcatgat gtgataacct gttcagtctg
     4441 tggttatttg ttgctaaaag tcgtctctag gcgactttta gggttaattt agtcgtctgg
     4501 agaccaccat gtcaagtggg tacgaaaaaa aactgaaaga gatacggaaa agtgaagggt
     4561 taactcaagc tgagtttgca gatgttactg ggataaatct cggaactata aagaattatg
     4621 agagcggtaa aagagaggtt ggtttaagcg ttgttgatcg cgtaattaat tctaaggatt
     4681 tcgaaaaata cactatatgg cttatgacgg gaaaaacaaa tgaggctgct gggcagatca
     4741 gtccctctct ctcccctgat gggccagaaa acacatcgtc ttctcaaaaa tcccgcaaga
     4801 ctggtacaca gcccggctaa tcatggaacg ctgggggcat ggtggtcttg taacgctggg
     4861 gtttcacgaa tgagcataaa atcaattccg ggagggtatc ttcttgacat gcgccctgag
     4921 gggcgtaaag gcaaacgcat tcgcaaaaaa tttaaaacga aatcggatgc agttttatat
     4981 gagcggtggg tgctggcgca acagcataac aatgagtgga aaggaaactc cattgatcgc
     5041 cgtccgctgt cggtgcttat tgacttgtgg tggaaatacc acggccagct aatgaagtca
     5101 gggcataaca cgcgccttaa attgctgcgc ttgagtgagg caatggatga cccgtgtgtg
     5161 cataaactta atacaacgat gctcaccgag ctacgtgtgt ccaggataga gcaggggata
     5221 cagcccagca ccattaatcg agagattggg gcgttaagcg cgatgtttac cgcactcatc
     5281 tcatccggcc attttcttaa cgataacccc gttcaaggcc ttaaaggaat gaaggttaac
     5341 gagcgcgaaa tgggatatct gagtaagtct gaatgtgttc agttgctgga tgcactggct
     5401 gaaaatcccg atgaacggct ggctgtcgaa atccttctgt cgaccggggc gcgatggggc
     5461 gaggtagcgg cactggagca gcgccgtgtt cttcattgtc gaatcacttt ttcaaaaacg
     5521 aagaacagca aaaaccgtac cgttcctatt tctgaaagcc tgtttgaaaa gatcaaaaaa
     5581 cggggcggga aactggtgtt tccgacgctg gattatccat tggttcgcga tgtcatcaaa
     5641 acggtcgcac ctgatgttcc tgacggccag gctgttcatg cgctgcgcca caccttcgcc
     5701 agtcatttca tgatgaacgg cggcaatatt ctgacgctcc agaaaattct ggggcacgca
     5761 aagattcaga caacgatgat ttatgcccat cttgcgccgg attacttgca ggatgcggtg
     5821 agatttaatc ctattgctgg gtaaggagga tgttgtggaa aaaacagtta atagcgattt
     5881 gcatttaggt cagtactgga gaatagacat tgtatgcgat ccagaacttc gagatgaggt
     5941 tgagcaatat ttttctctac acgatgttgg ttttagcaaa attgaagttt tttccgtcga
     6001 aaatccatat aaacttgctc tgttctttga tttcgcgaag aagggtgtgg aggttgcaaa
     6061 agccattatg ggactcttag accgaaatga tattgaaata acgatgtatc gtgctacgga
     6121 cagtagcccg cagtctgtaa aaagcatcaa attgcgtaag tctgaagacg tagagaaatg
     6181 taaagatttg cttagtacgt gtgtcgcaat tggcgttcaa cgaaataagg aaaaagaggg
     6241 gagcgaatga tccacaaagt gaccacatcc ctgttatttg ttgtggttgg ctgtgttttt
     6301 gtgtgtctgt aagtctttga taattatcta acttattgat ttttgtttgt gtttatggcc
     6361 gctctgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca
     6421 cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac aaaatattcg
     6481 ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg
     6541 tgaacaatgg atctcgccag gtgactggtt tactcctcgt ggcgaactgt tcgtctggca
     6601 aattcgcctg ccacgtacgc tggctgtatt gctggttggt gcggcgctgg ctatatccgg
     6661 cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac ttggcgtctc
     6721 taacggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc aactccccaa
     6781 ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc atcactttaa tactcttacg
     6841 tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg cattagggat
     6901 tatctgtagc gcactaatga cgtgggctat ctacttttcc acctctgttg atttacgtca
     6961 gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa gctggctgat
     7021 gctggcattg atccccgtgt tgttgtggat ctgttgtcag tccaggccga tgaatatgtt
     7081 agcacttggc gagatctcgg cgcggcaact gggtttaccc ctgtggttct ggcgcaatgt
     7141 gctggtggcc gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg gtgctatcgg
     7201 ctttattggt ctggtgatcc cacatattct tcggttgtgt ggtttaaccg atcatcgcgt
     7261 attacttccc ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg atattgtagc
     7321 gcgcctggca ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc
     7381 gccggtgttt atctggttat tgttaaaagc aagacgttag ccgcaaaaag acggtctatg
     7441 attaaaagct aagattttta ctgaccacac ccaggagaaa cgatgcaaga ttccattctg
     7501 acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta cgctggtaat
     7561 gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg
     7621 gagaatattc agaaagcctg ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac
     7681 cagtttctgg aacaagaacc gggcagcgat gaagagatta aaacttactg taccaccaca
     7741 tggggggtga cgttcccgat gttcagtaag attgaagtta atggcgaagg acgccatccg
     7801 ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg cgccggaaga gagcggattc
     7861 tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat tttatggaat
     7921 tttgagaaat ttttagttgg cagggacggg ttggtcatcc agcgtttttc cccggatatg
     7981 acgcctgaag atcccattgt gatggaaagc attaaactgg cgttggcaaa ataatgtcta
     8041 ttgtgatgca gttacaggat gttgcggaaa ctacccgcct ggggccgctt tctggcgagg
     8101 ttcgggctgg ggagatcctg cacctggtgg ggccgaatgg cgcgggtaag agtaccttgc
     8161 ttgcacgaat ggccggaatg accagcggta agggaagcat tcagttcgcg gggcaaccac
     8221 tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc ctacctttca caacagcaga
     8281 cgccgccgtt tgcaatgccg gtctggcact acctgacact gcatcagcac gataaaacgc
     8341 gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa ctcggacgta
     8401 gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg gtggtgctgc
     8461 aaatcacacc acaagccaat cccgcaggtc aattgctgct tcttgatgag ccgatgaaca
     8521 gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct gagcgctctg tgtcagcaag
     8581 gactggcgat tgtgatgagc agtcacgatc tcaaccacac attgcgtcat gcgcatcggg
     8641 cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag gtgctcacgc
     8701 cgccaaatct ggcgcaggcc tatgggatga attttcgccg tctggatatc gaaggtcaca
     8761 gaatgctgat ttcgaccatc tgatttcatt agtatcttga aaatcgggta attacgacgc
     8821 taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct gccttatttt
     8881 gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc caaatgccag
     8941 actttctgat tcgattaccg ttattgccgg tttgaacgac cagctacaaa gctggcatgg
     9001 cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt
     9061 tgtaacgatg cgcgatcgtt tcgatttgca gctgccccga gaaaccaaag aacaagcctc
     9121 tatcggcacg caaattgata aagacgagtt gctgcctggt gacctggtct ttttcaaaac
     9181 gggttccgga caaaatggtt tgcatgtagg tatttacgat accaacaacc aatttatcca
     9241 cgcctctacc agcaagggag tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa
     9301 tttctggcag gcgagacgaa tttagtatgt cgaaaaatgg acggcgatga tacatcgccg
     9361 tccatttact cctgaataca gtagtatttt gcagacaaaa attccgtaaa tggattattc
     9421 gtttataatc aggataatct aaaaaaagga cccctgaaaa agtggttaac atggaacaaa
     9481 tgaaagttac tccttatgta attgttatgt cttatggcct catccctttt agccggatac
     9541 tgaaaaacat ccttcgagag ggacggttac catgaagatt tttttggaga atctttatca
     9601 ttctgattgc tatttcctgc cgataagaga taaccagcaa gaccttgtcg gcgttgagtt
     9661 aattacccat ttctccagtg aagatggtac ggttcgtatc ccgacgagtc gggttatagc
     9721 gcaacttacc gaggagcaac attggcaatt attttcagag cagttggaat tactaaaatc
     9781 atgccaacat ttttttattc aacataaact ctttgcctgg cttaatttaa cgccacaagt
     9841 ggctacatta ttattagaga gggataatta cgcaggcgaa ctattaaaat atccgtttat
     9901 tgaactgttg attaatgaga attacccgca tctcaatgaa ggaaaagata atcgcgatct
     9961 tttatcctta tcgcaaatgt atccccttgt gttaggcaat ttgggagcag gtaatagcac
    10021 aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg ctggataaaa gttttattca
    10081 gcaacaaatc acgcatcgct ccttcgaacc gtttattcgt gcgatccagg cgcaaatttc
    10141 cccttgttgc aattgcatca tcgcgggggg cattgatacg acagaaatat tggcacagat
    10201 tacccccttc gatttccatg ctctccaggg atgcctgtgg cctgccgttc ctataaatca
    10261 gataacgacg ttggttcagc gataaccctc ctgtttgctg gtgtttaaga cgagagtaac
    10321 cgtctacact attaaacagg aggatctatg accctgtctt ttattacccg ctggcgcgat
    10381 gaattgccag caacctatac agcactttcc cctacgcctt taaataatgc ccggctgatt
    10441 tggcataata ccgaactcgc taacacgctg agtattccat cgtcgctgtt taaaaatggc
    10501 gcaggtgtct ggggcggcga aaccctactg cctggtatgt caccactggc gcaggtttac
    10561 agtggtcatc agttcggcgt ctgggcgggc caactgggtg atgggcgcgg tattttactc
    10621 ggcgaacaac tgcttgctga tggcactaca atggactggc atctgaaagg tgctggcctg
    10681 acgccttatt cgcgaatggg tgatggacgg gcggttttac gttcgacgat acgagaaagt
    10741 ctcgccagtg aggcgatgca ttatctgggc attccgacga cccgcgcgtt aagtatcgtc
    10801 accagcgatt cgccagtgta tcgggaaacg gtggagccag gcgcgatgct gatgcgtgtg
    10861 gcaccaagtc atctgcgctt tggtcatttc gaacattttt actatcgccg cgagccggaa
    10921 aaggttcgtc agttagctga ctttgccatt cgtcattact ggtcatatct tgaagatgat
    10981 gaggacaaat accgcctctg gtttagcgat gttgtcgcac gtaccgcatc gttaattgcc
    11041 caatggcaga cggtcggctt tgctcatggg gtgatgaata ccgataacat gtcgctgctg
    11101 gggctgacgc ttgattacgg gccgtttggt tttctggatg attacgaacc cggttttatt
    11161 tgtaatcact cggatcatca agggcgttat agctttgata atcaacctgc tgtcgcgttg
    11221 tggaatttac agcgtctggc gcagacattg tcaccatttg ttgccgtaga tgccctgaat
    11281 gaggccctgg acagctatca gcaggttttg ttgacgcatt atggacaacg gatgcggcag
    11341 aaactgggct tcatgacgga gcaaaaagag gataacgcgc tactgaatga attattcagt
    11401 ctgatggcgc gagagcgcag cgattatacc cgcacattcc gcatgctgag tctgaccgag
    11461 cagcacagcg cggcgtcacc gctacgtgat gagtttattg atcgtgcggc atttgatgac
    11521 tggtttgccc gttatcgggg gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag
    11581 cagctgatgc aaagcgttaa cccagctctg gtgttgcgca actggttggc gcaacgggcg
    11641 attgaagcgg cagaaaaggg tgatatgacg gaattgcacc gcctgcatga ggcgttgcga
    11701 aatcctttca gcgacagagc tgatgactat gtcagtcgtc cacctgactg gggtaaacgg
    11761 ctggaagtca gctgctcgag ttaaaggcta cttggtcaac agcagcttgc cagcctgagt
    11821 tttacggagc agatattctt gcccgtcatg atcgataatc agtttgccat ccggacctaa
    11881 cagtgtctgg ctggaaattc gccgaatagg ctgcggggaa gcggtcttgt gattggcatc
    11941 cgtttcaggc gtgagttttc tgctatccgt ataacgcata aaaattaata ataattgata
    12001 taacaatgat tcttattatc aatattatgg ctgaccagag caagcagtaa tgtgctgaga
    12061 agacaacaag ttgcgcagaa atgcccatcc catcaggaat gggcacgcat tcagaagcgg
    12121 gtatctaccg cagaggcaag tttttcgacc aggcgttcgg tatcttccca gcccagacac
    12181 gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct
    12241 tcgcgcagga aactttccgc cataattcca gcaatcgccg tagagccatt gcggatttgc
    12301 tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca gttaccgtgg
    12361 ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg
    12421 gcgatatcat cggcatgata attcggtttt ttgccgccac gcataataat gtggccatac
    12481 gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga gagaaacata
    12541 tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgagttgcc atcggtacca
    12601 tttttaaaac ctaccggaca ggagagtgcc gaagccattt cacggtggat ctgactttcg
    12661 gtagtacgtg cgccaatcgc gccccaactg attaaatcag caataaactg accggtcacc
    12721 atatcgagga actcagtcgc ggtagggaca cccagctcat ttacctgtaa aagtaatttg
    12781 cgcgccatct ccagaccgtg atttacccga tagctgccgt ttaaatccgg atcggagatt
    12841 agccctttcc agccgacaac agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc
    12901 agccgtgact ggtactggtt gcgcagcgac tgcagacggg tggcgtactc cattgcagcg
    12961 gtgagatcgt ggatcgagca ggggccaata atgaccaaca gtcgcttatc ttcaccattc
    13021 agtatttttt caattctgcg gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg
    13081 ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc agtacggagt
    13141 tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca ggactaccgg
    13201 gaagtgatgc gcatcacgat aaaccaatcg cagctgattt caagtgcgag gtgtgtcaca
    13261 cctcgccggg tggttagcat gataacaaaa aaataagcta atgcactagt tctctagtac
    13321 attcggcggc taagccccat gatatcgagg agcttggtgg caatctcttc taccgaataa
    13381 ttggtactgt taatccacgg gatctgattt ttacggtaca acgcttctac ttccgcgact
    13441 tccatcctgc actgacgaag cgaggcatag cggctgttct cccgacgttc ctcgcgaatc
    13501 gccgccagac gttccgggtc gatagtcagg ccgaacaatt tatgctgaag cggtttgagc
    13561 gacgcgggta gcaccagatt gtccatatcg tcggcaataa aggggtagtt tgccgcgcgg
    13621 ataccaaact gcattgccag atacagactg gtgggggttt taccacagcg agaaacaccg
    13681 agcaggatca cctgagcctg gtccagattg cgcaacgaaa tgccgtcatc gtgggcgagg
    13741 gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg gttaaggcca
    13801 tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag cggggcaacc
    13861 agcgcctgaa caatatcctg gcaaaagcct tcactttgca agatgatggc gcgaatctcc
    13921 ggcaacacga tggagtagaa gaccagcggg cgcacacctg tctggtgata aattgcgtca
    13981 atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag cgtaatgctg
    14041 ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc agttattgcc
    14101 gtaccatcag aaatataaaa aacgtggcga tcaacagcat tatccatttt gttcttcccg
    14161 tgatgcagac ataattctat gaaagcataa attaaaaacg cacagaagcg taggacgtaa
    14221 tgtctggttt ataaaatgaa ccttcaattt tattttttat gaaaacagca tttcattttt
    14281 atggtttcgt ttataccgat ggtttatgtg gaaattgtcg aatagagcag gtttgcgcaa
    14341 cgctgggatc agtcttaaaa agtaaaaaaa tatatttgct tgaacgattc accgtttttt
    14401 tcatccggtt aaatatgcaa agataaatgc gcagaaatgt gtttctcaaa ccgttcattt
    14461 atcacaaaag gattgttcga tgtccaacaa tggctcgtca ccgctggtgc tttggtataa
    14521 ccaactcggc atgaatgatg tagacagggt tgggggcaaa aatgcctccc tgggtgaaat
    14581 gattactaac ctttccggaa tgggtgtttc cgttccgaat ggtttcgcca caaccgccga
    14641 cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag cgcatttatg aactgctgga
    14701 taaaacggat attgacgatg ttacccagct tgcgaaagcg ggcgcgcaaa tccgccagtg
    14761 gattatcgac actcccttcc agcctgagct ggaaaatgcc atccgcgaag cttatgcaca
    14821 gctttccgcc gatgacgaaa acgcctcttt tgcggtgcgc tcctccgcta ctgcagaaga
    14881 tatgccggac gcttcctttg ccggtcagca ggaaaccttc ctcaacgttc agggttttga
    14941 cgccgttctc gtggcagtga aacatgtatt tgcttctctg tttaacgatc gcgccatctc
    15001 ttatcgtgtg caccagggtt acgatcaccg tggcgtggcg ctctccgccg gtgttcaacg
    15061 gatggtgcgc tctgacctcg catcttctgg cgtgatgttc tccattgata ccgaatccgg
    15121 ctttgaccag gtggtgttta tcacttccgc atggggcctt ggtgaaatgg tcgtgcaggg
    15181 cgcggttaac ccggatgagt tttacgtcca taaaccgaca cttgcagcga atcgcccggc
    15241 tatcgtgcgt cgcaccatgg ggtcgaaaaa aatccgcatg gtttacgcgc cgacccagga
    15301 gcacggcaag caggttaaaa tcgaagacgt accgcaggaa cagcgtgaca tcttctcgct
    15361 gaccaacgaa gaagtgcagg aactggcaaa acaggccgta caaattgaga aacactacgg
    15421 tcgcccgatg gatattgagt gggcgaaaga tggccacacc ggcaaactgt tcattgtgca
    15481 ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg gagcgttata cgctgcattc
    15541 acagggtaag attatcgccg aaggccgtgc tatcggtcat cgcatcggcg cgggtccggt
    15601 gaaagtcatc catgacatca gcgaaatgaa ccgcatcgaa cctggcgacg tgctggtcac
    15661 tgacatgacc gacccggact gggaaccgat catgaagaaa gcatctgcca tcgtcaccaa
    15721 ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt gaactgggca ttccggcggt
    15781 agtgggctgt ggtgatgcaa cagaacggat gaaagacggt gagaacgtca ctgtttcttg
    15841 tgccgaaggt gataccggtt acgtctatgc ggagttgctg gaatttagcg tgaaaagctc
    15901 cagcgtagaa acgatgccgg atctgccgtt gaaagtgatg atgaacgtcg gtaacccgga
    15961 ccgtgctttc gacttcgcct gcctgccgaa cgaaggcgtg ggccttgcgc gtctggaatt
    16021 tatcatcaac cgtatgattg gcgtccaccc acgcgcactg cttgagtttg acgatcagga
    16081 accgcagttg caaaacgaaa tccgcgagat gatgaaaggt tttgattctc cgcgtgaatt
    16141 ttacgttggt cgtctgactg aagggatcgc gacgctgggt gccgcgtttt atccgaagcg
    16201 cgtcattgtc cgtctctctg attttaaatc gaacgaatat gccaacctgg tcggtggtga
    16261 gcgttacgag ccagatgaag agaacccgat gctcggcttc cgtggcgcgg gccgctatgt
    16321 ttccgacagc ttccgcgact gcttcgcgct ggagtgtgaa gcagtgaaac gagtgcgcaa
    16381 cgacatgggg ctgaccaacg ttgagatcat gatcccgttc gtgcgtaccg tagatcaggc
    16441 gaaagcggtg gttgaagaac tggcgcgtca ggggctgaaa cgtggcgaga acgggctgaa
    16501 aatcatcatg atgtgtgaaa ttccgtccaa cgccttgctg gcagagcagt tcctcgaata
    16561 tttcgacggc ttctcaattg gctcaaacga tatgacacag ctggcgctcg gtctggatcg
    16621 tgactccggc gtggtgtctg aattgttcga tgagcgcaac gatgcggtga aagcattgct
    16681 gtcgatggct atccgtgccg cgaagaaaca gggcaaatat gtcgggattt gcggtcaggg
    16741 tccgtccgac catgaagact ttgccgcatg gttgatggaa gaggggatcg atagcctgtc
    16801 tctgaacccg gacaccgtgg tgcaaacctg gttaagcctg gctgaactga agaaataaaa
    16861 taaatccccg gcggcgttca gtcgccgggt ttatgtgatc cccgaagata aaacttattc
    16921 aatctcttca cagacatcct gcgttaatcg ccgcataata tcttttctta acaaaaactt
    16981 ttgtatttta cctgaggcag tgcgcggtag tttttcgatt accacgatat gttcaggata
    17041 tttatatttt gcgacccgtt tacggctaaa aaaagccacc acctcttcca gcgataatga
    17101 atgatgcggc gctttcagca cgacataagc gcatgatcgt tcacctaaac gttcatcggg
    17161 cattgcaacc acacaggcat cgtgaatttt aggatgctgc aataaaatat cttccacttc
    17221 acggctgcta atattttcgc cgccgcggac aataatatct tttttgcgtc cggtaatttt
    17281 tatatagcca gcctcatcca tacggcagag atcgccgctg taataccagc cttcttcatc
    17341 cagcgcacgg gcggttaatt caggttcatc aaaatatccc ataaacacat tggggccacg
    17401 cgaggcttct tcaccttcgc aacctggcgg taaggtcttg cgtgcgtcat cgaccacttt
    17461 aatctctaca cctgcggcag cgtaaccatc ggtgtgcata aagcgcgaca aaggatcatc
    17521 gagattcacc accgcatgcg gcgaactttc tgtggaacca taaacactta ataatttaat
    17581 gccgagctgc tggcattcac gcgccacttt tttggggatt gtggttccgc cgcaaagaaa
    17641 gaaacgcagc gctgacaggt ccgcgggttg tttctctagt acattcaaaa gatcatagac
    17701 aaacggcgtt gcgcctagca tacaggtgca acgctgctgc tcaagcagcg cgagacacgc
    17761 atcaggagtg aaaatatcta acaacacgct gcgagcgcca attaagaatg gtgccgttac
    17821 gccatgcaga aagcccgttg cgtggccgag tggcgcaggc atcataaaga catcctgcca
    17881 ggtcagattc agccgcgcgc aataagcccg ctcactggcg agaatattgt tatgcgttag
    17941 catcacgccc tttggcagac cctcggttcc ggaggtaaac agcaccgcag ctaattcatc
    18001 gccgtgggtc gttatcgccg tggtcagtgg ggtattgtcg gcgataatct gacttaatga
    18061 gagggaagag gtggcgggag ccagtttgtc cacgccgaca atttgttgta gttgtggaag
    18121 ctgattttgc agcggcagga ttaaatctac cgggcgcgtt tgtttaaaca acgtcggtgc
    18181 aaagaacatt tttgcctgac acttattgag cacccacacc agttctgctt cccgccagga
    18241 aggcaacagc ggcacggaaa ccgcaccgat tttcaggcag gcaagataga taacggtaaa
    18301 ttcacaccag ccaggcagtt gaaatgcgat gcgatcgcct gattcaatac ccttcgctaa
    18361 catccagttt gccagacagc tcgcggcgtg atcgagcgcg ctataggtgt acgatgcacc
    18421 atgattatcg accacggcaa ttttgtctgg catcgcacga gcggtctgct gccagtaatc
    18481 ggccagcgaa gcatcgcccc atagcccttg ctgacgatac gccgcacgac gttgttcgtt
    18541 gaacgttaat gtcactttca tgttggactc tcgaaacaga acatcaggcc cgagatgcgg
    18601 gcctgtggga tgcatgatta gccgtagcga aaatcaatac cgaaggtgcc tgcgggatat
    18661 tgccactgtt cgagaatagt gttaccgcac agcacccgac aggtgccgca ctccagacaa
    18721 ccggcagaat caaaatgaat gttgccggcg tcatcctgct tataaagtcc ggcagggcag
    18781 gctttcatta atttacggaa ttcattgata tcgggatttg ccgccagaat gatatgcggg
    18841 tggccctcat caacatggaa tttattgacg cctaatttga tgtcgacgtt aaccgtagcg
    18901 ttctggctca tagcgcggtt gctcccttaa tgccatcttt cagcaagttg atcagcccaa
    18961 ttttcttcgc atgtcccatg atcattttgc gtaccggctg gtttggtttg ccgtcaatgg
    19021 tgaacatctc gttcatgatg tcggcgacca ttcgtgggta ttggctaaac aggcgcgggt
    19081 tttccatcag cgccgggatc ttgcggaaat gctgcatatc gcgcatgatg cagctttgtt
    19141 ccagctcacg tttgtattgc gccagactgc tggcggagaa atcctcgcgt tctttggcgg
    19201 cgatcaccgt tgtggcggca gcctgagccg atgcaatggc taaatccatg ccacgaactg
    19261 taaaacccaa attcaggcag aagcctgcgg cgtcaccaac gatcatcacg ccatcgttaa
    19321 ccatctgcgg caccattgcc agaccgcctt ctggcaccat atgcgcggaa tattcaagca
    19381 gtttgccgcc gctaattagt gggcgaatgg cggggtgttg tttaaaatct tccagcattt
    19441 gcggcacgct tttttgcgca tgggcgatat cacccaatcc acaaaccagc cccaaggata
    19501 tggaatcctt gttggtatag agaaatcccc cgcccatcag gccgtcagaa ggggaaccgg
    19561 caaacagcca ggcggcacct tcctcgcccg taatattaaa gcgatcgttg atctgttctg
    19621 gtgtgaggcc aataacctct ttaacaccaa cagcgtaatg atgcggatcg gaagcgggaa
    19681 ccattcccag cgagcggcca agcatcgagt taacgccatc agccagaatc accacattcg
    19741 cttcgagaat atcatcccca gcctgcacgc cagtgacctt gtttccttca cgaaccagcg
    19801 catcgacgcg aacgcctggg ataaactgcg cgcccgcctg ctcggcttgt tccatcaacc
    19861 acgggtccag acgattacgc aatacggtat aagatgcgtg ttgcggaaca tctggttgct
    19921 cgcggtgaaa atcgagggta acggcgcttt cttcggttaa gaaggagatt ttctcgcgtg
    19981 tgaccttgcg ttctaccggc gctgatactg caaagcctgg aatgattgct tcaagtgtgt
    20041 gggcataaag acgcccgccg gtcatgtttt tacatccggc actgtcgccg cgttctatca
    20101 ccaggacatc cagcccggct cgcgccatga ccagtgcggc aacgctacca gcaacgcccg
    20161 caccgaccac aatggcatca aatttgtctt ccgacatgcg ttatgctccc ttcccgctgc
    20221 gtttacagcg ggagatgtgg ttgaaagggg aattagcggc tcaactggct aatcagggca
    20281 gggacgactt tgtagatatc gcccaccaga ccgtagtcgg cataattgaa gattggcgca
    20341 tttttatctt tattgatggc gacaatcact tttgcgccgt tgccgccaac catatgctgg
    20401 atctgcccgg agatccccag cgtcaggtag agatcggatt tcagcaacac gccggagaca
    20461 ccgatataac gttcacgctc catccagttc tcgccttcgg caattggacg tgaacagccg
    20521 acttcagcat tcagcaccgc cgccagttcg tggaccattt tcaggtcatc ctgcgccgcc
    20581 agaccacgac cgacgccaac cacacgtttt gctttgctca ggtccacgct gcttgcggct
    20641 ttcgcacggc gttcgcgaca gagaatttca tgacgcggag caacataagg taccgtttct
    20701 gtcgggcact gatgagaggt atcactggtg cacggttcct gaacaccggg tgcaagggta
    20761 atgatcgcca gcgggctgtt gatcttttcc tgagcgaacg ctaacccgcc atacatccgg
    20821 tgttcggcgc aaatgtgacc atcgacaata tccaccgccg tggcatcgtt caccagcgcc
    20881 gcattcagtt gcacacttaa ccgtgctgcc agagctttac cacgtttcgt cgcggccaac
    20941 agcaacatag cggggtgttt atctttcagc agggcagcaa tgctttcggc gtaattttca
    21001 gtgcgttgca gcgcgtcgtt ttgctcaaga acataaatac attttggacc ataaggcata
    21061 actgcctgcg cctggtcggt attttgtaca atggcataca cctgttggcc ccattgctga
    21121 gcgccgccaa acagttcagc ataacgttca ggattatcgc taaagaccca gacgctgttt
    21181 aattgactca tcgtttcttc tccgtaggct tcagttcagg gctttcgtca gatgttccgc
    21241 cagctcggca atggcctccg gcgaatcgtt atcgataatg atgtgcttac gttctgtttg
    21301 cggcggtacg cgaatgccga ccagttcagc aagtggcgcg ctctggctcc agtcaatatc
    21361 acttgcctgc cactgattta ccggtttttt acccgcgccg agaatggctt tcatcgaagg
    21421 aatgcgtggc acgttaatat cggaggtgac gcagagcacg gctggaacag agagttcaat
    21481 aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt gtattgccct gacgctgaat
    21541 agcactcact gcattaatca ccggaagttg cagaatttct ccgaccagca agccaacctg
    21601 ctgggcataa aggtcgccgg aaccttcacc aaagatcagt aaatcgaagc cgatcttttc
    21661 aattgctgcc gccagcgctt ttgcggtatc gagaggcagt gcatgttcaa gttgcgcatc
    21721 ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg tctttgcgca ctttcgagtt
    21781 ctgcaacaat gagccgccaa cggtcagcgc ggctatctca tcgtcatctg ttgcgagctg
    21841 gcttgcagct tcaatggcat tgagatcgaa ctggctgatt ttggcgtcgg cattgtcgaa
    21901 attcagggtg tattctggag tgacaacaat gtcctgttct tcaggcacca gcttaaagca
    21961 ggttattatt ttcatgattt ctcctgtgag tcgcatacgg aaccatctcc gatggaaggt
    22021 aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt tttcgacgga gcaagctgtt
    22081 ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa aacaaaagtg caatttgcgg
    22141 caatatagtt attccatgac cgtacaatct tgcattcagt gtaaataatc ttgtttagct
    22201 ctcgctaaac gcggttgtat aaccgcaata cacgccgtat tttatccggc atatattgtt
    22261 tttgcttgct catggaatag ggaggttgtc atgtatcaac gctgttttga taacgccagt
    22321 gaaacgctgt ttgtcgccgg taaaacgcca cggctttcac gttttgcatt tagcgatgat
    22381 ccaaaatggg agtccggaca tcacgttcat gacaatgaaa ccgagttgat ttacgtcaag
    22441 aaaggggttg caaggtttac catcgattct tcgttatatg tcgcacatgc ggatgacatt
    22501 gtggtgatag aacgcggcag gctacatgcg gtggcctctg acgttaacga tccggcaacg
    22561 acgtgtacct gtgcgctgta cggctttcag tttcaggggg ctgaggaaaa tcagctactg
    22621 caaccgcatt cttgtccggt aattgccgcg gggcagggaa aagaagtcat aaaaacctta
    22681 tttaatgagc taagtgtgat tttgccgcaa agtaaaaata gccaaacatc ttcgttatgg
    22741 gatgcatttg cctatacgtt agccattctt tactacgaaa attttaaaaa tgcttatcgt
    22801 tcggagcagg ggtatattaa aaaagatgtt ctgataaaag atattctttt ctatctgaat
    22861 aataattatc gcgaaaaaat cactttagaa cagttatcga aaaaatttcg tgccagcgtc
    22921 agttatattt gccatgaatt taccaaagag tatcgtattt cccctattaa ctatgttatt
    22981 caacggcgta tgacggaagc aaaatggtca ctcactaata ctgaattatc acaggcagag
    23041 atctcctggc gtgtgggtta tgaaaatgtc gatcactttg ccaaactgtt tttgcgccat
    23101 gtcggttgtt cgcccagcga ttaccgtaga caatttaaaa actgttttgc ggaacaagaa
    23161 atcctatctg aatttcctca accggtaagt cttgttggat aaatctggcg gcgcagtttg
    23221 ttaagaagct gcgccgcctg cagattattt gttctgatag tctttcagga tctgccgacc
    23281 tgctacgtaa atcataattt cgtctgtacc gccgccgata cgttcacaac ggacatcacg
    23341 ccagaagcgg gagacgcgcg cttcatcggt atagcccaga ccgcccatga tttgaatcgc
    23401 atcatcaatg acttccattg cggtacgtgc gcaatacagt tttgccagcg ccgcgctggt
    23461 gcgcagtgac tgatgctgat cggcttgcca tgccactttc agcaccatgt tgcgcatgtt
    23521 gtcgatctta atcgccatca gcgccagttt ttcctggatc atctggttat gaccaatggg
    23581 cttaccaaaa gcgatacgtt ggttggcata gcgggcggca tcttcaaagg cgcattcggc
    23641 aaagccggtg ctgcgcgcgg cgttgatcag gcgctccatc tcaaagttgt acatcacatt
    23701 gaggaaaccc attccttctt cgcccaccat gtcgctctct tcaacttcca cattgtcgag
    23761 atagacttcg caggtgctga gcatatgcca gccgatttta tgcagcgggt taatcttaat
    23821 gccgggctta ctggagtcga cccaccacag ggtgaaggct tttttgggat ctttcggttg
    23881 cggatcgcgc gccagcacca gcatatacgg gtactctttc gcactggtaa taaaggtttt
    23941 ttgtccgttg atgtagacct tgccgttttt acgcgtgtaa gtggtagtgg cactgttgtt
    24001 atctgagcca gcacctggtt ccgtcaacgc cagggcatag gcgggatcac ccgtttccag
    24061 ggtgctctct gccgttttac gtagctgctc tgcagaaccg aaacggcgca tactgtgaat
    24121 acattgaccg ttggtaatta aaaatgccgg agcaccgcat tttgacactt ccatcagcgc
    24181 cagcatttgg gtgacgtaat ccgcagggat accaccaaat tcttccggca cgccaagcat
    24241 ggaaataccg ttatccgcca gcgcccgcat aaactcacgc ggatatgtcc cgttttgatc
    24301 gcaggtgcgg aaatactctt ccggaaagtt agtcgtaatc agttcgcgaa tactggccag
    24361 cagcagttct tgttcttcag ttaaagaaaa atccatcatt atactcctgt taatgagccg
    24421 cttcaggcag gacaaaaccc atcgccgcat cgataaataa tctttcgtcc atcagtttga
    24481 gttctggcga aatcactggg gtgaagtcca ttttgtcgag aatatctttt tgtaaatcga
    24541 cgccaggggc gatttcgatt aaatgcaggc cgtcttcttt cagcgtgaat acagcacgtt
    24601 cggtgatgta acgaacatcc agtccccgtt cgagcgcgat ttttccgcta aaagtaattt
    24661 ccggtagttc ccgaataaat ttatttaccc gtccttcctg gacgatatgc aatttgccat
    24721 cggcaatttc tgtttttaaa ctgcccgcag ttaacgtgcc gcagaaaata attttcttcg
    24781 aggtggcact gatatcaata aatccaccgg tacccatgat tttgccattg aatttatgca
    24841 cgccgacgtt accgtgctgg tcgacttcag caaaactcaa ataacaaaca tccagaccac
    24901 caccgtgata aaaatcaaac tgggacgtca tatccagaat ggcgcgggta ttcacgttcg
    24961 cgccaaaggc gataccctgt gaagtaattc cgccaatcgg acctgtttct accgtcagaa
    25021 taaagtcatc agcacaacct tcttcccggg cgaccaggcc aataccgtca gcaataccga
    25081 cgccgacatt ccccaccgcg cctttacgca tttcgaataa tgcacgccgc gcaactaatt
    25141 tacgttggtt taggggcagg ctaagtttgt gctgtcatca agggtgaagt caccagaaat
    25201 aaaacggtta accggcgcac cgccatacag ttggctttga tccggatcga ccaccacaat
    25261 atccaccaga taacccggaa tacggacaga tttaggatgc agcgtggctt tcttaaccat
    25321 tttctgcacc tgcatcatca caataccgcc gttattgtgc accgcctggg caataaccaa
    25381 tgcgtcgaga tacatcacct catcttcaaa agtggcgtag ccttcactgt cgcaggtggt
    25441 agcgcgaatg aaggcgatat ctggcgcaat cgctttgtaa tagagatatt ctttgttatc
    25501 aaactcgacc agtttaatca ggtcttcttt cgtgacttca ttcagtttgc cgccttgctg
    25561 gcgtggatcg acaaatgtcc cgatgccaat atcactaata ataccaggct ggtgggctgc
    25621 ggcggcgcgt aaggtttgtg taagtacacc ttgtggatag ttataagcaa taattttatt
    25681 ttgttctgcg agttcagaaa tacgcggcga ttgtccccag tgaccacata atgcccattt
    25741 caccagacct tcttgcgcca gaggactaat accacggtcg gcgcgatcgc caagccctgt
    25801 cggactaata atcgataaat tacgtggcgt ttgagtctgt ttatatttat cagcaagagc
    25861 tgtaattaac gtggtggctt ccagaatacc gccgccagcg cctaacacac aaagtgttgc
    25921 ttcgtcggga atataattca ccgcttcctg tgccgacagg accggcactc gtccattaat
    25981 acgaggtggt tttacaggtt tcattgttaa ttcctgattc agatgtgatt ttccctacgc
    26041 cagatggaga attagcgcac agagactcac gaaatatttt tattaaatac ggttaaatat
    26101 attttttaag ttcggcgggg agggtgttcc cgccgaaata ttattgctta tgcctggtgt
    26161 aaaatagtta ataccgtgcg taaatcattt accgagattt gccctggtgc agaggctttt
    26221 tttaccgcac caaaagttgc cgccgagcca aatacttcac cagccagacg agaaattacg
    26281 ccagtttttg ccatcgacat cgtgatgatt ggacgatcgg catactgctc ctgcatctcc
    26341 agggtcgcgg taagcaacgt cagcacatcg ctggtacttt gcggcatcag cgcaatctta
    26401 ggaatatcgg cgtcgaagga ctgcattttg cgcagacggg caatgatttc ttcggcttcc
    26461 ggcgttttat ggaagtcatg gttggacatg acaactttca catcatgcgc gtgggcgtag
    26521 gcgacggttt ctttaacctg atcatcgccg gtaaataact ccagatcgat catatcaacc
    26581 aggccgctgt cgatggctgc acgattgaga gcaatataag cctcggtgga aatcgcctgc
    26641 tcgccgcctt ctttggcact gcggaaggta aacagcagcg gtttttctgg catggtttca
    26701 cggagaattt ttgccgccgc catgacagac tccacattgg agaggtcggc aaagtggtcc
    26761 acacgccatt ccagaatatc aaagtccgct tcacgatagg cgagagcttc ggatttcacg
    26821 cgggcgatat ctttcgccat cagcgagacg atgattttag gtgcgcccgt accaatgacg
    26881 agatctttta cagttacggt tttcattttt taccctttct gcacgcggtc agcctgtcag
    26941 gcaccgaacc ccatgacctg tttaacatat tccagaggga aatctttgcc agtccataac
    27001 gtgaactgtt cagccccttg ccacaacaac atgccgtatc catcaatcgt tttgcaacca
    27061 gcttgttgcg cctgctgcaa taacttcgtc atatgcgggt tatacacgca ttcagtgacc
    27121 agcagtcccg gatgtaacag actgatatca ttaactaatg attcattctc aaggggtttc
    27181 atacccactt ttgtgccatt ggttaaaatg tcggcggaag ccagggcttc agcaaaggct
    27241 tgctgatcgg cgagatcggt aaccgtgacg acacaatcgg tgttttcgtt aacccgctgc
    27301 gcgaaggcga gggctttatc gaagaactca tcccgacggt taaagagttt aatttctttt
    27361 aaaccttcaa ttgccccctg cgcgccaatc gccgttgagg cacctccggc ccctaacagc
    27421 accattgttt tgcctttgat atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg
    27481 ccgtcggtgt tatagccgcg caggtagcca tcatcattaa cgatggtgtt gatggcaccc
    27541 accagtttgg ctgctggcgt taattcatca acatattcac acgccagttg tttgttcggc
    27601 attgatatac cagttccgcg cattttgagg gcttttaatc cttcaattgc tccgggaaaa
    27661 ctatcgttat ccacttcaaa ggccatatag gtaaatggca atcccgcttt ttctaaggct
    27721 ttattctgca tttcaggcga taaactgtgg cggataggat aggccatcag cccaatcaat
    27781 tcgtattttg cggtaacatc cataattaac tcctttaacc tctatgctta attgtgttta
    27841 accgggtgct aaatttacgc tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc
    27901 ggtaatagcg gataaaaaca ataattgcgg taatcagggc cagcagcgcg aaagtaaaat
    27961 cgagaacaat gatatattga aggccgatgt tcgacagata accggtaatc agcggaataa
    28021 caaagttagc cagtccaccc atcatcatat aaatactggt gactttggct ttgctttttg
    28081 ggaaaaactc tgacatgacc gaaacaccga gctgtaaaat gccgccagct gctgagaaac
    28141 cgataacaaa ggcgccggca ttacacacca gtggagaagg gtacaggtag ataatggctg
    28201 ctgttattgt tgccagtgca gagttaaata cattagccca gatgggccgg accatttttt
    28261 tcagtagtgc ggcaaaaata aagacacaga ccaacgagcc catactgtaa taagagatgg
    28321 tttttaatgc ctcagcttct gacataccag caaaagccat cgcatatttg ggcatccaca
    28381 ccacaatcac ataaaaggtc gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt
    28441 ccagccagac taacggtttg ctgttcattt gcggtaattc attagctacg ctggcgtcca
    28501 ccaactggct ggggaatttg cttttcaaca gcatcagcgt gatcagtaca aacagaatac
    28561 ccggaataat cagcccatag ccgtaccaga tattattgag caacatatag ctcaccagca
    28621 ttgggtagaa catttgccca aatgacacca tcgctttaac cagtatgacc gccgaaccag
    28681 aggctttcgg aaagcattcc atgagcgcgg ggtagccacc cgtatccagc gctgagttag
    28741 cgatacctac gcacactgcc agaccgtagg cgagagttaa attcgggcaa gcgggaatac
    28801 caaagaagaa tagcagatac attattactg ccattaatat caccgcccga cgaccaaact
    28861 tatcggagat cacaccgaag aataaaatac tgatcaatcg ccccaaacca ataccggaaa
    28921 ttaagtaggc aatgcccgcg ttgtcagtgg aaaacttttc cgccagagat gacatgtttt
    28981 gggcaagcgt aataacacta atgccgtgca ggaagtagct gaagtaaata caaagaacag
    29041 ccaggataaa tggcgtgctg aaagccttat tttgagacat tttcacagct ccttgctgaa
    29101 agataatttc gttaccgtac gtattgttct tcgacggttt acataattac tatactgaag
    29161 atgattttcc ctgggtacat ggcataaagt ttatgtttcc aggaatattg catgtcatag
    29221 tttgtttttg atcgcgattt tccttagtta gcgcagaaag agttatttat ccgtaagatc
    29281 ttgcatgacc tgaaaagaat attgcattac ccacccggag cgacattttc ctttagcgag
    29341 tgatgccgcg tttttttgcg gctgcgtaag ccgataaaca gtgccagtaa aaaaccgatg
    29401 gcagcgatgg cggtatcgaa ccacataata tcggcaatac ttctttggga cagatgagcc
    29461 gtaatcaacg gaatagtaaa ggtcgcaata ctgcccgcac tgtaatagat ccctgtagct
    29521 ttacctttag cgtaagggaa acgttcagcc attaacgtca ggccaatttg cacaacacct
    29581 ccagcagagg taaaaccaat gacaaaagca aatattatca ccacataaaa tgtgggatgc
    29641 aggcagacgg taaacagagc aataaatgag ataaaggtgt acagcatcag taatgttgtt
    29701 gggcgaacgg tattacgaat gagtggagcg gtaataaata cacaaagcag cgaatccacg
    29761 gtgtagatac tgagtagttt aatcgacata gtgtatgaca tgcctgcaac aaattgtccg
    29821 tactgtgcca gccactggct aaccagataa aacgttgcca ttgagatata gccatataag
    29881 gtataactgg ctaaatcgat aattgaacag cgatgttccg tagagctggt ggttttcttt
    29941 atgacaggta agcgatgacc cggatggggt gggaacgtac aacggtataa aaacagagcg
    30001 ttaataaaca taatgcctgc agcaatcata aaggaccaac cgaaccacag ttcagcccac
    30061 actaacaggc taatgattag cggtaataaa aattgtccgc tggaaacaaa tgctttaatt
    30121 aaaatattgg ctgtcccagg tgagcgtgga aaagcttcca tcaaactggg ataagtgcct
    30181 gcatcgagaa aactgtttgc cattcctgcc agaaagccaa aaacataagc gataatgatg
    30241 ttattggtgt gcaggatgcc aaaaaagaag gccatatagc agcacatccc gagcatgata
    30301 aaagggcggc gaccaaagcg atcggataat aatcctgcaa aaagcaagac acttaatcga
    30361 ccaatgccca gcgatgagat aactatcgag acacccgcgg cattagtctg ccaaagtgtc
    30421 tccagcgagg ccatattcag gctcatcaaa atgacgccca taccatgcac taggtaatta
    30481 aaatacaacc caagtgcggt agggtaatag ggatttttca taggttctac ttagcaaagg
    30541 tgctttaaaa agtgtagcga tgttgtgtca gtatccgttc attattgttg taacaacgac
    30601 aattcctttt tgtggagaaa ttaacaaagc cgctccagat cgtgtgcata taattcagcg
    30661 gctaccgatt gatagatctt ccagtcaata aaattgccat cagggtagac ttgttgaaac
    30721 gcggttaagt cggggaaaaa acgcatggtg ttgttgccga tgcggttatt tattttctct
    30781 atgatagcat gaagatgttt ttttcgttgc tgacgcatag ccaacagttg ctcgactaca
    30841 cgatcaggaa tggcgcattt gccattttcc cactgacgcc agctttcact gtcaccggtc
    30901 tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa aaatatggcg aagtgcctgg
    30961 agttcataag cgttcatcgt gtaatggctg catgaaataa acaataaaat ataaataaca
    31021 ttatcagcct gctgacggct tgaagagagt cacagtatct tgtgcaacat tattggaaag
    31081 agtgaggggc gaggtagcga agcggaaaaa tgttaaaaaa aagcccatcg tgggagatgg
    31141 gcaaagacta cacacagcaa ttcgttgttt cactcagggg atttccatgc ttataaatca
    31201 atatgttgat ttataaccgt aagctaatag taggcatacg gactttttgc gtcgatcaga
    31261 ttcgtctcat tagtttaaaa actgtaaaga atttgcgaca ggtactacca gctgaatggc
    31321 ggtaagcagg aaaatatggg gaagagcaag caaatatcaa ttagcggggg ttaacaatca
    31381 ggcggcagag ccgcctgatg agaaattact tattcggttt ctcgatttcg ccaagttctt
    31441 cgagaatttc ttccggttgg tccgtcggcg gcgggacttc ttccacccac gcggcaaaca
    31501 gacgccagga aacggctaac agaaccggac caataaacag accgatcatc ccgaaagcaa
    31561 tcaaaccacc aataacacca gagaggatca ggatcagcgg taaatcggca cccatgcgaa
    31621 ttaacattgg gcggatgacg ttatccagcg tgccaaccac accgctccac actaacaata
    31681 ccgttcccca ggtggtatcg ccagtccagt agagccagat aatcgccgga atcagtaccg
    31741 gcaacgggcc aagctggaca aggcaggaga ggatcattaa caccgttagc aacgttgcat
    31801 aaggtacgcc ggatacggcg aggccgatac cgccaagcac tgcctgtact aacgccgtca
    31861 ccaccacacc cagcgccacc gcgcggatag cctgtgccgc cagcagtacg gcggcatctc
    31921 cgcgaacgcc tgccagacgg gttgcaaaat ggcgaatgcc ttgtgccacc tgttcaccgc
    31981 gccagtacag cagggcactg aagagaagca tcagcgcaca atgcaccata aagcgcccga
    32041 tatgcgccgc ctgcccaacg aaccaggtgg tggtggtgcc aatataaggg cggactttcg
    32101 ccatgatcgc cgtccccccc atatccagca agttgtgcca gcctgcatac agcttcgcgc
    32161 caatcaccgg aatggtatta agccacgcca gatcgggtaa cgtcatgtcg ccgctggaaa
    32221 tggctttaat tagcgggccg ctgccgtcga cgatactgtt aaccagcaaa gcaataggga
    32281 tgataaacac catcactaat aacagcgtca tcaccagaac ggcgagagag cggcggccaa
    32341 acatgatctt ttgcaaacgt aacaataccg gccaggtggc gataaccacc gtaccggccc
    32401 atgcaaagcc gagaataaag ggttgaacaa tccacagaca tgccacaatc atgatggcta
    32461 aaaacagcac cgaaagcaga atttgtgcga catccctggg ctgacgaaca tttaccataa
    32521 ctacttttca ccttttttgt gcgtcaaatc gtcgacgcga cgtgaacacg cgaaactcac
    32581 ctgcataatg atgagcaatt tcagcggttt ttaacaggcc gattctgccc ttaattctga
    32641 tgggcgtgtt agaaaaaaat gtggtacaac aactcaggca acacgcaaac gatttactcg
    32701 tcgtatttca agttgcatgt gctgcggcta cgttcgttca ccccagtcac ttactattgt
    32761 aagctcctgg agttcactcg cttgccgcct tcctgcaact cgaaatccat agagtaaata
    32821 aacatcacct ttcagtgcgc tattgcagcc gcctgaaaga atagtgtatc gacctacatc
    32881 acagaccgca ggaaagggtc aatataatga ttccacagat ttcccaggca cccggcgtcg
    32941 ttcaactggt gcttaatttt ttgcaagagc tggagcaaca aggttttacc ggcgatacgg
    33001 cgacaagtta tgccgatcgt ctgacaatgt cgaccgacaa cagtatttac caacttctcc
    33061 ccgatgcggt ggtatttccg cgttcaaccg cagatgtggc gctgatcgcc cgtcttgccg
    33121 cgcaggaacg ctattcatcg ctgatcttta ccccccgtgg cggcggcacc ggcactaacg
    33181 gtcaggcgct caaccagggg attattgttg atatgtcccg ccatatgaac cgcatcatcg
    33241 aaattaaccc tgaagagggc tgggtgcgcg ttgaggccgg ggtgataaaa gatcaactca
    33301 atcagtacct gaaaccgttc ggctactttt ttgcgccgga actttcgacc agcaaccggg
    33361 caacgctcgg tgggatgatc aataccgatg cttctggtca gggatcgctg gtctatggca
    33421 aaacgtcgga tcacgtactt ggcgtacgtg cggtgttgtt ggggggcgat attctcgata
    33481 cgcaaccttt acccgtcgaa ctggcggaaa cgctgggtaa atccaatacc acaatcgggc
    33541 gaatttataa cacggtttat caacgttgcc gtcagcaacg ccagttaatc atcgacaact
    33601 tccccaaact taaccgcttt cttaccggtt acgatctgcg tcatgtcttt aacgatgaga
    33661 tgaccgagtt cgacctgacg cgcattctga cgggttcaga agggacgctg gcctttatta
    33721 ccgaagcgcg gctggatatt acgcccttgc ctaaagtgcg ccgtctggtg aacgtcaaat
    33781 atgactcttt tgactccgcg ctgcgtaacg cgccgtttat ggttgaggcg cgggcgcttt
    33841 cggtagagac ggtggactca aaagtgctga atctggcgcg ggaagatatt gtctggcatt
    33901 ccgtcagcga gttgattacc gatgtgcctg acaaagagat gctcgggctg aacattgtgg
    33961 agtttgctgg tgatgatgag gcgctgattg acgagcgagt taatgctctc tgtgtgcggc
    34021 ttgatgagct gattgccagc caacaggcag gtgtgattgg ctggcaggtg tgccgcgagc
    34081 tggcgggtgt tgaacgtatc tatgcgatgc gtaaaaaagc cgttggtctg cttggcaatg
    34141 ccaaaggtgc cgctaagcca attccgtttg ctgaggatac ctgcgtaccg ccggaacatc
    34201 tggccgatta tattgctgaa tttcgcgcgc tgctcgacag ccacggctta agctacggta
    34261 tgttcggtca cgtcgacgca ggtgtcttgc acgtccgtcc agcgctggat atgtgcgatc
    34321 ctcaacaaga gattttgatg aagcaaatct ctgatgacgt agtggcgctg actgcgaaat
    34381 acggtggttt gttgtggggc gagcacggca aaggttttcg cgctgaatac agcccggcgt
    34441 ttttcggtga ggaacttttt gcagaactgc gcaaagtgaa agcggcattt gacccgcata
    34501 accgactcaa cccagggaag atttgcccgc cagaaggtct cgatgcgccg atgatgaaag
    34561 tggacgcggt gaagcgcggt acattcgatc ggcagatccc cattgcggta cgccagcagt
    34621 ggcgcggtgc gatggagtgt aacggcaacg gtttatgctt caactttgat gcccgtagtc
    34681 cgatgtgtcc gtcgatgaag atcacccaga accggattca ttcaccgaaa gggcgcgcaa
    34741 cgctggtgcg tgaatggctg cgtttgttgg cggatcgcgg cgttgatcca ctcaaactgg
    34801 aacaagaact gcctgaatcc ggcgtcagtt tgcggacgtt aattgcccgc acgcgcaata
    34861 gctggcatgc gaataaaggc gaatatgact tctcacacga agtcaaagag gcgatgtcgg
    34921 gctgtctggc ctgtaaagcg tgttcgaccc agtgccccat caaaattgat gtgccggagt
    34981 ttcgctctcg ttttctgcag ctctatcaca cccgttattt acgcccgctg cgcgaccacc
    35041 tcgtcgctac ggtcgagagc tacgcgccgc tgatggcacg cgcgccgaaa acctttaact
    35101 tcttcattaa ccagccgctg gtgcgcaaac tctcggaaaa acatatcggc atggttgatt
    35161 tgccgctgct gtcggtcccc tcgctacaac aacaaatggt ggggcatcgc tcggcaaaca
    35221 tgacgctgga acagcttgaa tctctcaatg cagagcagaa agcgcgcaca gtgttggtgg
    35281 tgcaggaccc ctttaccagc tattacgatg cgcaagtggt ggcggatttt gtccgtctgg
    35341 tcgaaaaatt aggtttccag cctgtgttac tgccattttc gccaaatggc aaagcccagc
    35401 atattaaagg ttttcttaat cgttttgcga agacggcgaa aaagacggcg gatttcctca
    35461 accgtatggc gaagctgggt atgccaatgg tgggcgtcga tccggcgctg gtactttgtt
    35521 atcgcgatga atataaactg gcgctgggcg aggaacgtgg cgagtttaac gtcttactgg
    35581 cgaatgaatg gctggcaagc gcacttgagt cacagccagt ggctacagtc agcggtgaat
    35641 catggtattt ctttggtcac tgtaccgaag ttaccgcctt gccgggtgcg ccagcacaat
    35701 gggccgcgat atttgcccgt tttggcgcga aactggaaaa tgtcagcgtg ggttgctgcg
    35761 gcatggcagg gacttacgga catgaagcga aaaaccataa aaattcgctc gggatctatg
    35821 agttatcctg gcatcaggct atgcagcgac tgccgcgtaa ccgctgtctg gcgaccggat
    35881 attcctgccg tagccaggta aaacgggttg aaggcacggg ggtacgccat cctgtgcagg
    35941 ctttactgga gattattaaa tgatatggaa acggaaaatc accctggaag cactgaatgc
    36001 tatgggtgaa ggaaacatgg tggggttcct ggatattcgc tttgaacata ttggtgatga
    36061 cacccttgaa gcgacaatgc cagtagactc gcggacaaag cagcctttcg ggttgctgca
    36121 tggaggagca tccgtggtac tggccgaaag tatcggttcc gttgccggtt atttatgtac
    36181 cgaaggtgag caaaaagtgg ttggtctgga aatcaatgct aaccacgtcc gctcggcacg
    36241 agaagggcgg gtgcgcgggg tatgcaaacc gttgcatctc ggttcgcgtc accaggtctg
    36301 gcagattgaa atcttcgatg agaaagggcg tttgtgctgt tcgtcacgat tgacgaccgc
    36361 cattttgtga cgttgtcacc agaaaagtgt gacgcatatt gcctttggtc attatctgca
    36421 cagtaaacgg tggtatactg tgcaggtctt gtctaaaagc gaggatgcta tgtctactca
    36481 acttgatccc acccagctgg ccattgaatt tttacgccgt gaccagagca atctttcacc
    36541 tgcccaatat ctcaaacgtc tgaaacaact agaacttgaa tttgctgacc tgcttactct
    36601 ctcttctgct gaattaaaag aagaaatcta ctttgcctgg cgtctgggcg tgcattgatc
    36661 ctgacttaat gccgcaagtt cgccaaatcg acctggatta acctccgtcc aggttcgatg
    36721 accttcttaa tactgcttat ctaccgcctg atattgttca aaaataatac taatcgcagt
    36781 ttgtgttaaa acggcgggtt agctttatga gattattctt atcgcccctt caagagctaa
    36841 gccactgaga gtgccggaga taagcgccgg atggggtaga aacccttaag cctgtgttgc
    36901 acagacttaa gggttttctt atttctgaac gctgcacttt tttacctatc tcttaaatag
    36961 tcattttcaa tacaactttt tctttttcat cacagcctta atgctcctta gataataacc
    37021 attatctaac aatgagatac ctaattctta gcgtgcctgt taacccacac atcagggtct
    37081 atgcttatta aaagcaggca aaaagcatgt tttacggtaa agcccctgcg tttgctgggt
    37141 tgtactgata atcattatca ctaacatgct gttatacgct gaaagcgatg aagtgaggta
    37201 aatcgatgga catgcattca ggaaccttta acccacaaga tttcgcctgg caaggcttaa
    37261 cgctgacacc cgcagcggcg atacacatcc gtgagctggt ggcaaagcag ccgggtatgg
    37321 tcggcgtgcg cttaggcgtg aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca
    37381 gtgttagcga gccggacaaa gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg
    37441 tcccgctgca agcgatgccg tttattgatg gcacggaagt cgatttcgtt cgtgaaggac
    37501 ttaatcagat attcaaattt cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa
    37561 gctttggggt ataggcggta ctatgtctcg taatactgaa gcaactgacg atgtcaaaac
    37621 ctggaccggc ggcccgctga attataaaga aggattcttc acccagttag ccaccgatga
    37681 gctggcaaag gggataaacg aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc
    37741 ggagtggatg ctgaagtttc gtctaaacgc ctatcgcgca tggctggaga tggaagaacc
    37801 gcactggttg aaagcgcact acgacaagct gaattatcag gattacagct actactcagc
    37861 accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa cctggcgcgg tgcagcaaac
    37921 tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg tttgagcagt tgggcgttcc
    37981 cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc gactcagttt cggttgccac
    38041 tacttatcgc gaaaaactgg cggagcaggg aattattttc tgttcctttg gtgaggcgat
    38101 ccacgatcac ccggaactgg tgcgtaaata tctcggcacc gtggtgccgg ggaatgacaa
    38161 cttctttgcc gcgcttaatg cggcggtagc ctctgacggt acgtttattt atgtgcctaa
    38221 aggcgtgcgc tgcccgatgg aactttccac ctattttcgc attaacgcgg aaaaaaccgg
    38281 gcagtttgag cgcaccattc tggtggccga cgaagacagc tacgtcagct acattgaagg
    38341 ctgttccgct ccggtgcgtg acagctatca gttacacgcg gcagtggtgg aagtcatcat
    38401 ccataaaaac gccgaggtga aatattccac ggtacaaaac tggttccctg gcgataacaa
    38461 caccggcggt attctcaact tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa
    38521 aatgtcatgg acgcaatcag aaaccgggtc cgcgattacg tggaaatatc ccagctgcat
    38581 tttgcgcggc gataactcca ttggtgagtt ttactcagtg gcgctgacca gcggtcatca
    38641 gcaagcggat accggcacca agatgatcca catcggtaaa aacaccaaat cgaccattat
    38701 ctcgaaaggg atctctgccg gacatagtca gaacagttat cgcggcttag tgaaaatcat
    38761 gccgacggca accaatgcgc gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa
    38821 ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat aacagcgcac aactggagca
    38881 cgaggcaacg acatcacgta ttggtgaaga tcaactgttt tactgcctgc aacgcgggat
    38941 cagcgaagaa gacgccatct cgatgattgt taacggtttc tgcaaagacg tgttctcaga
    39001 actgccgctg gaatttgccg ttgaagcaca aaaactcctc gccatcagtc ttgaacacag
    39061 cgtcggataa ggaataaaca tgttaagtat taaagattta cacgtcagcg tggaagataa
    39121 agctatcctg cgtggattaa gcctcgccgt tcgtcccggc gaagttcacg ccattatggg
    39181 gccaaacggt tcgggcaaaa gtaccttatc ggcaacgctt gccgggcgcg aagattatga
    39241 agtgacgggc ggcacggttg agttcaaagg caaagatttg cttgcactgt cgccggaaga
    39301 tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg gtggagattc caggcgtcag
    39361 taaccagttt ttcctgcaaa cggcacttaa tgcagtgcgc agctatcgcg gccaggaaac
    39421 gctcgaccgc tttgattttc aggatttgat ggaagagaaa atcgctctcc tgaagatgcc
    39481 ggaagattta ttaacccgtt cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa
    39541 cgatattttg caaatggcgg tgctggaacc ggagttatgc attcttgatg agtcggactc
    39601 cgggctggat attgacgcat taaaagtggt cgccgatggc gtgaactcgc tgcgtgatgg
    39661 caagcgctca ttcatcattg ttacgcacta ccaacgcatt ctcgactaca tcaagcctga
    39721 ttacgtccat gtgctatatc ggggacgaat tgtgaaatcc ggcgatttca cgttggtcaa
    39781 acaactggag gagcagggtt atggctggct taccgaacag cagtaacgcg ctgcaacagt
    39841 ggcatcactt gtttgaagct gaagggacaa aacgctcacc gcaagcacag cagcatttac
    39901 aacaattgct gcgtaccgga ctgccgacac gtaaacatga aaactggaaa tatacgccgc
    39961 tggaagggct gaccaatagc cagtttgtca gcattgcggg agagatatcc ccacagcagc
    40021 gagatgcctt agcgttaacg ttagacgctg tgcggctggt atttgtcgat ggacgttacg
    40081 tgtcagcact gagcgatgcg actgaaggca gcgggtatga agtgagcatt aacgacgacc
    40141 gtcagggggt acccgacgct attcaggcgg aagtgtttct gcatttgacg gaaagcctgg
    40201 cacaaagcgt gacgcatatc gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt
    40261 taatgcatat tacccagggc gtggcaggtg aagaggtcaa cactgcccat taccgacatc
    40321 atctggatct ggcggaaggt gccgaagcaa cggtgatcga acattttgtc agccttaatg
    40381 atgctcgtca cttcaccggc gcacggttca ctatcaatgt cgcagcgaac gcccacttgc
    40441 agcatatcaa gctggcgttt gaaaacccgg tcagtcacca ctttgcccat aacgatttgt
    40501 tgctggctga cgatgccacc gcatttagcc atagtttcct gctgggtggc gcagtgttac
    40561 gacacaacac cagtacgcaa ctcaatggcg aaaacagcac gctgcggatc aatagcctgg
    40621 cgatgccggt gaaaaacgag gtgtgtgata cccgtacctg gctggaacac aataaaggtt
    40681 tttgtaacag ccgacagttg cacaaaacta tcgtcagcga caaaggccgc gcggtattta
    40741 acggtttgat caacgtcgcg cagcacgcca tcaaaacgga tggtcagatg accaacaata
    40801 atctgctgat gggcaaactg gcagaagtgg atacgaaacc gcagctggaa atctatgcag
    40861 atgatgtgaa atgcagccac ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct
    40921 atctgcgctc gcgcgggatc aatcagcagg atgcccagca gatgatcatt tacgcctttg
    40981 ctgccgaact gacggaagca ctgcgtgatg aggggcttaa acagcaggtg ctggcccgaa
    41041 tcggtcaacg gctgccagga ggtgcaagat gactttttcc gtcgacaaag tgcgggccga
    41101 ctttccggtg ctttctcgtg aggtaaacgg tttgccgctg gcttatctcg acagcgccgc
    41161 cagtgcgcag aaaccgagcc aggtgattga cgccgaggcc gagttttatc gtcatggcta
    41221 cgcggcggtg catcgcggta ttcatacctt aagcgcccaa gcgaccgaga aaatggagaa
    41281 cgtgcgcaag cgggcatcgc tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt
    41341 ccgcggcacg acggaaggga tcaatctggt cgccaatagc tggggtaaca gcaatgtgcg
    41401 ggcgggcgat aacatcatca tcagtcagat ggagcaccac gctaacattg ttccctggca
    41461 gatgctttgc gcacgcgttg gcgcagagct gcgtgtgatc ccgctcaatc ccgacggtac
    41521 gttgcaactg gagacgctgc ctacgctgtt tgatgagaaa actcgcctgc tggcaattac
    41581 tcatgtctcc aacgtgcttg gcacagaaaa tccactggcg gaaatgatca cgcttgcgca
    41641 ccagcatggc gcaaaagtgc tggtggatgg cgctcaggcg gtgatgcatc atccggtgga
    41701 tgttcaggcg ctggattgcg acttttacgt gttctccggg cataaactgt atggccccac
    41761 cggaattggc attctttatg tgaaagaagc cttgttgcag gagatgccgc cgtgggaagg
    41821 gggcggttct atgatcgcca ccgtcagcct gagtgaaggc acaacctgga ccaaagcacc
    41881 ttggcggttt gaagccggta cacccaatac cgggggcatc attggtcttg gcgcggcgct
    41941 ggagtatgtt tcggcgctgg ggcttaataa catagccgag tatgaacaga atctgatgca
    42001 ctacgcgcta tcacagctgg aatctgtacc ggatctcact ctctatggtc cgcagaacag
    42061 gcttggcgtt attgctttta atcttggtaa acaccacgcc tatgatgttg gcagttttct
    42121 cgataactac ggcattgctg tgcgtaccgg acatcactgc gctatgccat taatggccta
    42181 ttacaacgtc cctgctatgt gtcgggcgtc gctggccatg tataacaccc atgaagaagt
    42241 ggatcgtctg gtgaccggac tgcaacgtat tcaccgtctg ctgggataac agggaggcac
    42301 tatggcttta ttgccggata aagaaaaatt gctgcgtaat tttttacgct gcgccaattg
    42361 ggaagagaaa tatctctaca ttattgagct gggccagcgt ctgccagaat tacgcgacga
    42421 agacagaagc ccacaaaata gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg
    42481 ccagaatgcg cagggaatta ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg
    42541 gcttattgcg gtcgtcttta ttctctacga tcagatgacg ccgcaggata ttgtcaattt
    42601 cgatgtgcgt ccgtggtttg aaaaaatggc gctcacccaa catctcaccc catctcgttc
    42661 acaaggtctg gaagcgatga ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac
    42721 taaagggaca gctttcatcc ggttggctca cattgccgga tggtcaatgt ttacactgac
    42781 ctgcacagga gtgtttcggt ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag
    42841 tctccggcaa atacaggagg tttacaatga aacgcgcgtc tttgcttaca ctcacgctta
    42901 tcggcgcttt tagcgccatc caggctgcct gggcggttga ttatccgcta ccaccaaccg
    42961 gaagccgact ggttgggcaa aatcaaacgt atacggtgca ggaaggggat aaaaaccttc
    43021 aggccatcgc ccgacgtttt gatactgcgg caatgttgat ccttgaagcc aataacacta
    43081 tcgccccggt gccaaaaccc ggtacgacga taactattcc ttcgcaactg ttattacctg
    43141 atgcgccgcg tcaggggatt atcgttaacc ttgcagagtt gcgcctttat tattatccgc
    43201 cgggagaaaa tatcgtgcag gtttatccga taggtattgg cttgcagggg ctggaaacgc
    43261 cggtgatgga aacgcgtgtg gggcagaaaa tccctaaccc aacctggacg cctactgcag
    43321 gcattcgtca acgttcgctg gagcgtggta ttaaattacc gccagtcgtt cctgccggac
    43381 caaataaccc gctaggacgt tacgcactgc gcctcgcgca tggtaatggc gaatacctca
    43441 ttcatggcac cagtgcgccg gacagcgtcg gtttgcgcgt cagttcaggg tgtattcgca
    43501 tgaatgcacc ggatattaaa gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag
    43561 tgatcaacga acctgtgaaa tattccgtgg agcctaacgg gatgcgttat gttgaagtac
    43621 atcgaccact atcggcagaa gaacagcaga acgttcagac aatgccatac acactgccag
    43681 caggctttac gcaatttaaa gacaataagg ctgtagatca gaagttagtc gataaagcgt
    43741 tgtatcgtcg ggcagggtat ccggtttcgg tgagcagtgg agcaactccc gcagccagca
    43801 atgcgccttc agtagagtca gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg
    43861 cgacgcagta gcggtaaacg gcagacaaaa aaaatggcgc acaatgtgcg ccatttttca
    43921 cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg ctggttagca
    43981 cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac gtcgttgctc
    44041 agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc gattttagcg
    44101 ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag taccagttta
    44161 gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg ttacaagtat
    44221 tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta tttttgatcg
    44281 ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaattgagag aaaacaggat
    44341 gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg atttgatttt
    44401 ttattgcttc tggttatcga ttaaataaaa aaagcgccca tcagggcgct tcgatataca
    44461 aattaattca caaaagcaat attacaggac gtgaacagat gcggtgttag tagtgccgct
    44521 cggtaccagt gcaccagaaa ccataactac aacgtcacct ttgtgtgcca gaccgctctg
    44581 cagagccagt tctttaccca gacggtagaa atcatcagta gaagtgatct ctttaaccag
    44641 ctgcggcaca acgcctttgc tcagtaccaa ctgatgagcc gttttttcgt tggtggtcag
    44701 tgccaggatg gtggcatccg ggaagtattt acgtactgcg cgagcagatt taccgccctg
    44761 agtagcaacc acgatcagcg gagcatccag tttttcagca gtttcaacgg caccacggca
    44821 taccgcttcg gtaatgcgca gtttacggtt gtcattgttg aactcgagac ggctgttcat
    44881 cacgcggtcg gtacgttcgc aaatggtcgc catgatagaa accgcttcca gcgggtattt
    44941 accttttgcg gattcaccag acagcatcac tgcatcagta ccgtcgagga tggcgtttgc
    45001 aacgtcaccg gcttctgcgc gagtcgggcg tgggtttttg atcatggaat ccagcatctg
    45061 ggtcgcagtg ataacgactt tacgtgcacg gatacatttt tcgatcatca tcttctgggc
    45121 gaagataact tcttctaccg ggatttctac acccaggtcg ccacgcgcaa ccatgatgcc
    45181 gtcagaggct tcgaggattt cgtcgaagtt gttgaggcct tcctggtttt cgattttgga
    45241 gatgatgtgg atgttttcgc cgccgtgcgc tttcaggtgc tcacggattt cgataacgtc
    45301 agaacgctta cgaataaagg aagcagcaac aaagtctacg ccttgttcgc aaccaaagat
    45361 caggtcctgt ttgtcttttt cagccagtgc tggcagagca atggaaacgc caggcaggtt
    45421 cacacctttg ttttcgccca ggtcaccgtt gttcagcact ttacagataa ctttgttacc
    45481 ttcaatggcg gtaacttcca taccgatcag accatcgtca accagtacgg tgttgccaac
    45541 tgacaggtca gtagtgaaac cttcatacgt taccgcaacc atttcgctgt tgccgataac
    45601 agatttatca gtggtgaaag taaaggtctg accagctttc agagaaacgt cgttaccgcc
    45661 ttccagtttc atggtgcgga tttccggacc tttggtatca agcaggatag cggcggtttt
    45721 accagttttg ctcatcacgt tgcgcagatt ctgaatgcgc tgaccgtgtt ctgcatagtc
    45781 accatgagag aagttcagac gcataacgtt catgccagcg tccagcattt tagctaacat
    45841 ctcttcagat tcggttttcg gtccgatggt gcaaacaatt ttggtctttt tcatgacagt
    45901 cttagtcttt aagttgagaa ggatgggaga aacttgcttt ctgggcgcac tgccggaaag
    45961 cgaatccggt attacgaaag tggtggtgcc tcgctctaag gataggtgac atcgaaaaag
    46021 cgtgcagagg aatgtgtgct tgtgtctcag cccaacgtga gtgcctgttt ttgagtcgtt
    46081 gagttctaca gtccaaagtg ctgaaaccat tcaagagtca attggcgcgc attatacgct
    46141 gaaattaatc caaaaggaaa acaaaaacag cgtttcaaaa aatatacaac ttgttagaca
    46201 ataaattgtt atcaaagatt taacgttcca gggaaaaggt tacgcaaaaa cttcgctgtt
    46261 tgcgcaacgt ctggtgccaa agttgtcagt gtgaggataa gtgaaagttc atccggtgta
    46321 taaatatatc atcgctcaat tgctatagtt aaaataattt atcgcgttat ttgtctgatt
    46381 ttgtttgatt tttgtttaat tggttagatt gatatatgct gatagttggc atgataaata
    46441 ggtataaaga atgggaaatc ggacaaaaga ggatgaactg taccgagaaa tgtgcagagt
    46501 tgtcggtaaa gttgtgctgg aaatgcgcga tctggggcag gaaccaaagc atattgttat
    46561 cgcaggcgta ttacgtacag cattagccaa taaacgcatt cagcgcagtg agctggaaaa
    46621 gcaggcgatg gaaaccgtca ttaacgcact ggtaaaatga ccattcagga tgaggcggta
    46681 agcttcatcc tgatattctt gcttcagttg cggtccagtt aatcattcta aataacaact
    46741 gctgaaatta tacgaatcgc g
//
LOCUS       DAZFKM010000023        45714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFKM010000023 DAZFKM010000000
VERSION     DAZFKM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            236..862
                     /gene="ydhY"
                     /locus_tag="ACHKAY_001413"
     CDS             236..862
                     /gene="ydhY"
                     /locus_tag="ACHKAY_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO5700118.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            883..2985
                     /gene="ydhV"
                     /locus_tag="ACHKAY_001414"
     CDS             883..2985
                     /gene="ydhV"
                     /locus_tag="ACHKAY_001414"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO5700119.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2998..3636
                     /gene="ydhW"
                     /locus_tag="ACHKAY_001415"
     CDS             2998..3636
                     /gene="ydhW"
                     /locus_tag="ACHKAY_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO5700120.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3700..4368
                     /gene="ydhX"
                     /locus_tag="ACHKAY_001416"
     CDS             3700..4368
                     /gene="ydhX"
                     /locus_tag="ACHKAY_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO5700121.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4365..5150
                     /gene="phsC"
                     /locus_tag="ACHKAY_001417"
     CDS             4365..5150
                     /gene="phsC"
                     /locus_tag="ACHKAY_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO5700122.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5154..5966
                     /gene="ydhT"
                     /locus_tag="ACHKAY_001418"
     CDS             5154..5966
                     /gene="ydhT"
                     /locus_tag="ACHKAY_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO5700123.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5978..7582)
                     /gene="ydhS"
                     /locus_tag="ACHKAY_001419"
     CDS             complement(5978..7582)
                     /gene="ydhS"
                     /locus_tag="ACHKAY_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO5700124.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7708..8013)
                     /gene="ydhR"
                     /locus_tag="ACHKAY_001420"
     CDS             complement(7708..8013)
                     /gene="ydhR"
                     /locus_tag="ACHKAY_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO5700125.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8121..8197)
                     /locus_tag="ACHKAY_001421"
     tRNA            complement(8121..8197)
                     /locus_tag="ACHKAY_001421"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8161..8163),aa:Val,seq:gac)
     gene            complement(8202..8278)
                     /locus_tag="ACHKAY_001422"
     tRNA            complement(8202..8278)
                     /locus_tag="ACHKAY_001422"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8242..8244),aa:Val,seq:gac)
     gene            8586..9842
                     /gene="ydhQ"
                     /locus_tag="ACHKAY_001423"
     CDS             8586..9842
                     /gene="ydhQ"
                     /locus_tag="ACHKAY_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO5700126.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9883..11256)
                     /gene="mdtK"
                     /locus_tag="ACHKAY_001424"
     CDS             complement(9883..11256)
                     /gene="mdtK"
                     /locus_tag="ACHKAY_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO5700127.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11471..12112
                     /gene="ribE"
                     /locus_tag="ACHKAY_001425"
     CDS             11471..12112
                     /gene="ribE"
                     /locus_tag="ACHKAY_001425"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO5700128.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12152..13300)
                     /gene="cfa"
                     /locus_tag="ACHKAY_001426"
     CDS             complement(12152..13300)
                     /gene="cfa"
                     /locus_tag="ACHKAY_001426"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO5700129.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13591..14802)
                     /gene="punC"
                     /locus_tag="ACHKAY_001427"
     CDS             complement(13591..14802)
                     /gene="punC"
                     /locus_tag="ACHKAY_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO5700130.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14915..15847
                     /gene="punR"
                     /locus_tag="ACHKAY_001428"
     CDS             14915..15847
                     /gene="punR"
                     /locus_tag="ACHKAY_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO5700131.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15844..16869)
                     /gene="purR"
                     /locus_tag="ACHKAY_001429"
     CDS             complement(15844..16869)
                     /gene="purR"
                     /locus_tag="ACHKAY_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO5700132.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17168..17257
                     /gene="cydH"
                     /locus_tag="ACHKAY_001430"
                     /gene_synonym="ynhF"
     CDS             17168..17257
                     /gene="cydH"
                     /locus_tag="ACHKAY_001430"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO5700133.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17423..18592
                     /gene="ydhP"
                     /locus_tag="ACHKAY_001431"
     CDS             17423..18592
                     /gene="ydhP"
                     /locus_tag="ACHKAY_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700134.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18738..19319)
                     /gene="sodB"
                     /locus_tag="ACHKAY_001432"
     CDS             complement(18738..19319)
                     /gene="sodB"
                     /locus_tag="ACHKAY_001432"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO5700135.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19447..20274)
                     /gene="mepH"
                     /locus_tag="ACHKAY_001433"
     CDS             complement(19447..20274)
                     /gene="mepH"
                     /locus_tag="ACHKAY_001433"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO5700136.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20609..20956
                     /gene="grxD"
                     /locus_tag="ACHKAY_001434"
     CDS             20609..20956
                     /gene="grxD"
                     /locus_tag="ACHKAY_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO5700137.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21007..25623)
                     /gene="lhr"
                     /locus_tag="ACHKAY_001435"
     CDS             complement(21007..25623)
                     /gene="lhr"
                     /locus_tag="ACHKAY_001435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO5700138.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPDGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25716..26363)
                     /gene="rnt"
                     /locus_tag="ACHKAY_001436"
     CDS             complement(25716..26363)
                     /gene="rnt"
                     /locus_tag="ACHKAY_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO5700139.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26466..26873)
                     /gene="gloA"
                     /locus_tag="ACHKAY_001437"
     CDS             complement(26466..26873)
                     /gene="gloA"
                     /locus_tag="ACHKAY_001437"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO5700140.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26954..28051)
                     /gene="nemA"
                     /locus_tag="ACHKAY_001438"
     CDS             complement(26954..28051)
                     /gene="nemA"
                     /locus_tag="ACHKAY_001438"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO5700141.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28088..28687)
                     /gene="nemR"
                     /locus_tag="ACHKAY_001439"
     CDS             complement(28088..28687)
                     /gene="nemR"
                     /locus_tag="ACHKAY_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO5700142.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28790..29029
                     /gene="ydhL"
                     /locus_tag="ACHKAY_001440"
     CDS             28790..29029
                     /gene="ydhL"
                     /locus_tag="ACHKAY_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO5700143.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29078..29974
                     /gene="ydhF"
                     /locus_tag="ACHKAY_001441"
     CDS             29078..29974
                     /gene="ydhF"
                     /locus_tag="ACHKAY_001441"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO5700144.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30055..30576
                     /gene="sodC"
                     /locus_tag="ACHKAY_001442"
     CDS             30055..30576
                     /gene="sodC"
                     /locus_tag="ACHKAY_001442"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO5700145.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30577..32589)
                     /gene="ydhK"
                     /locus_tag="ACHKAY_001443"
     CDS             complement(30577..32589)
                     /gene="ydhK"
                     /locus_tag="ACHKAY_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5700146.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32589..33446)
                     /gene="ydhJ"
                     /locus_tag="ACHKAY_001444"
     CDS             complement(32589..33446)
                     /gene="ydhJ"
                     /locus_tag="ACHKAY_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO5700147.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33449..33685)
                     /gene="ydhI"
                     /locus_tag="ACHKAY_001445"
     CDS             complement(33449..33685)
                     /gene="ydhI"
                     /locus_tag="ACHKAY_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO5700148.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33886..34320
                     /gene="slyA"
                     /locus_tag="ACHKAY_001446"
     CDS             33886..34320
                     /gene="slyA"
                     /locus_tag="ACHKAY_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO5700149.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34367..34834)
                     /gene="slyB"
                     /locus_tag="ACHKAY_001447"
     CDS             complement(34367..34834)
                     /gene="slyB"
                     /locus_tag="ACHKAY_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO5700150.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35108..36217
                     /gene="anmK"
                     /locus_tag="ACHKAY_001448"
     CDS             35108..36217
                     /gene="anmK"
                     /locus_tag="ACHKAY_001448"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO5700151.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36315..36644
                     /gene="mliC"
                     /locus_tag="ACHKAY_001449"
     CDS             36315..36644
                     /gene="mliC"
                     /locus_tag="ACHKAY_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO5700152.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36703..37359
                     /gene="pdxH"
                     /locus_tag="ACHKAY_001450"
     CDS             36703..37359
                     /gene="pdxH"
                     /locus_tag="ACHKAY_001450"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO5700153.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37488..38762
                     /gene="tyrS"
                     /locus_tag="ACHKAY_001451"
     CDS             37488..38762
                     /gene="tyrS"
                     /locus_tag="ACHKAY_001451"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO5700154.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38821..39684
                     /gene="pdxY"
                     /locus_tag="ACHKAY_001452"
     CDS             38821..39684
                     /gene="pdxY"
                     /locus_tag="ACHKAY_001452"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO5700155.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39728..40333)
                     /gene="gstA"
                     /locus_tag="ACHKAY_001453"
     CDS             complement(39728..40333)
                     /gene="gstA"
                     /locus_tag="ACHKAY_001453"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO5700156.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40439..41941)
                     /gene="dtpA"
                     /locus_tag="ACHKAY_001454"
     CDS             complement(40439..41941)
                     /gene="dtpA"
                     /locus_tag="ACHKAY_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO5700157.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42552..43187)
                     /gene="nth"
                     /locus_tag="ACHKAY_001455"
     CDS             complement(42552..43187)
                     /gene="nth"
                     /locus_tag="ACHKAY_001455"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO5700158.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43187..43882)
                     /gene="rsxE"
                     /locus_tag="ACHKAY_001456"
     CDS             complement(43187..43882)
                     /gene="rsxE"
                     /locus_tag="ACHKAY_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO5700159.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43886..44506)
                     /gene="rsxG"
                     /locus_tag="ACHKAY_001457"
     CDS             complement(43886..44506)
                     /gene="rsxG"
                     /locus_tag="ACHKAY_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO5700160.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44510..45568)
                     /gene="rsxD"
                     /locus_tag="ACHKAY_001458"
     CDS             complement(44510..45568)
                     /gene="rsxD"
                     /locus_tag="ACHKAY_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO5700161.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45569..>45714)
                     /locus_tag="ACHKAY_001459"
     CDS             complement(45569..>45714)
                     /locus_tag="ACHKAY_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFO5700162.1"
                     /translation="AKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVV
                     NED"
BASE COUNT        11523 a        11298 c        11911 g        10982 t
ORIGIN      
        1 ttcttgcttt gtaattttaa ttgataacga tcaatgttaa gggctcagaa taatcacatt
       61 attttttctc ctaaccataa aggattagtt tatcggcgtt aaatcttttc tcatacacta
      121 tcgggttagg gaaaatatct ggtgttgggg acagagtcag cagcgtatta gttgaatgct
      181 taatatcagg ctgctgaatt gtcagcactg tgtttgcgaa gaggatgcga aataaatgaa
      241 cccggttgat cgtccactat tagatattgg cttaacgcga ttagaattct tgcgtatatc
      301 aggaaaaggc cttgctggtc taacaattgc ccctgcgtta ctgtcacttt tgggttgcaa
      361 acaggaagat attgatagcg gcacagtagg gttgataaat acacccaaag gggtgctggt
      421 gacccagcga gcgcgatgca cgggttgcca ccgctgtgaa atctcttgta ccaacttcaa
      481 cgatggctca gtagggacat tcttctcccg tatcaaaatc caccgcaatt atttctttgg
      541 cgacaacggg gttggctctg gcggcggcct gtatggcgat ctcaactata ccgcggacac
      601 ctgccgtcaa tgcaaagagc cgcaatgcat gaacgtctgc ccgattggtg cgattacctg
      661 gcagcagaaa gaaggctgta ttaccgtcga tcataaacgc tgtattggct gtagcgcctg
      721 tactacggca tgcccgtgga tgatggccac cgtaaatacc gaaagtaaaa aatcctcgaa
      781 atgtgtgtta tgcggtgaat gcgcaaacgc ctgcccaaca ggggcgttaa aaattatcga
      841 gtggaaagat attactgtgt gaatcttgca aaggaaaaaa tcatggctaa cggttggaca
      901 ggtaatatat taagagtcaa tctcacgaca ggaaatatta ccctcgaaga ttccagtaag
      961 tttaaaagtt ttgtcggtgg catgggcttc ggctacaaaa ttatgtatga cgaagtaccg
     1021 ccaggcacga aacctttcga tgaagcgaat aaattagtct ttgctaccgg cccattaact
     1081 ggatctggtg ccccctgtag ttctcgcgta aatatcacct cactttctac ttttaccaaa
     1141 ggaaatttag ttgtcgatgc ccatatgggt ggcttttttg cagcgcaaat gaaattcgct
     1201 ggatacgacg tcattattat cgaagggaag gcgaaatcac cggtatggct gaagattaaa
     1261 gatgacaaag ttagcctgga aaaagccgat ttcttatggg gaaaagggac gcgcgcaacg
     1321 acggaagaaa tttgtcgatt gaccagtccg gaaacctgtg tggcggctat tggtcaggct
     1381 ggggaaaacc ttgttcctct ctctggcatg ttgaatagcc gtaaccacag cggcggtgcg
     1441 ggaactggcg caattatggg ttcgaaaaac ctgaaagcga ttgcggttga agggacgaaa
     1501 ggggtcaaca ttgccgatcg tcaggagatg aagcgtctca acgattacat gatgactgaa
     1561 cttattggtg cgaataacaa ccatgtcgtg ccaagtacgc cgcaatcgtg ggcagagtat
     1621 tcagatccca agtcacgttg gacagcacgt aaagggctgt tttggggcgc ggctgaaggt
     1681 ggtccgattg aaacggggga aattccgcca ggcaatcaga acacggtcgg ctttcgtacc
     1741 tataaatccg tttttgactt aggtccggcg gcagagaaat acacagtaaa aatgagtggc
     1801 tgccactctt gcccgatccg ttgtatgacc caaatgaata ttcctcgggt gaaagagttt
     1861 ggcgtgccca gcacaggtgg taacacttgt gtagctaact ttgtccatac caccatcttc
     1921 ccgaacgggc cgaaagattt tgaagataaa gacgatggtc gtgtgattgg taacctggtt
     1981 ggtctgaatt tgttcgatga ctacggccta tggtgtaact acgggcagct gcatcgcgac
     2041 tttacttatt gttacagcaa aggggtgttc aagcgtgttc tgccagctga agagtatgcg
     2101 gaaattcact gggatcaact ggaagcgggt gacgttaatt tcattaaaga tttttactac
     2161 cgtctggcgc atcgtgtggg tgagctgagt cacctggctg atggttcata tgccatcgca
     2221 gaacgctgga atttgggtga agagtactgg ggctacgcaa aaaataaact ctggtcgccg
     2281 tttggctatc cggttcacca tgccaatgaa gcgtcagcgc aggtcggttc cattgttaac
     2341 tgtatgttca accgtgactg catgacgcat acccatatca attttattgg ttcgggtttg
     2401 ccattgaaac tgcagcgtga agtggcgaaa gagctttttg gttctgaaga tgcctacgat
     2461 gaaaccaaaa actacacgcc aatcaatgac gcaaaaatta aatatgcaaa gtggtcgctg
     2521 ttgcgggtct gtttgcataa cgccgtcacc ctgtgcaact gggtctggcc aatgaccgtt
     2581 tcgccgctga aaagccgtaa ttaccggggc gatctggcgc ttgaagccaa attcttcaaa
     2641 gcgatcaccg gcgaagagat gactcaggaa aaattagatt tagctgcaga gcgtatcttt
     2701 acgttgcatc gtgcctacac ggtaaaactg atgcaaacca aagatatgcg taatgaacac
     2761 gatcttatct gttcctgggt attcgacaag gatccgcaga tcccggtctt tactgaaggt
     2821 actgacaaaa tggatcgtga cgatatgcat gcttcgctca cgatgttcta caaagaaatg
     2881 ggctgggacc cacagcttgg ttgtccgacc cgcgaaacat tgcagcgtct ggggctggaa
     2941 gatattgccg ccgatctggc agcacacaat ctactgcctg tgtaaggaat gggtgagatg
     3001 aaccatcggg atgaattgcc gttagcgaaa gtcagcgaag ttgacgaggc gaagcggcaa
     3061 tggttacagg ggatgcgtca ccccgttgat acggtaaccg aaccagaacc ggctgaaatc
     3121 ctggcagagt tcattcgcca gcattctgcg gctgggcagc tggtggcgcg cgccgtgttt
     3181 ctctcgccac cctattcggt tgcagaagag gagctgtcag ttttgctgga aagcattaag
     3241 cagaacggag actacgcgga tatcgcttgt atgacgggaa gccaggatga ttactattac
     3301 tcaacgcagg ccatgagtga aaactacgcg gcgatgtctt tgcaagttgt ggagcaggac
     3361 atttgtcgcg ccattgcaca cgccgttcgt tttgagtgcc agacatatcc tcgtccttac
     3421 aaagtcgcca tgctgatgca agcgccatat tacttccagg aagcgcagat tgaagccgcg
     3481 atcgccgcga tggatgttgc acctgaatat gccgatatac ggcaggtgga gtcatcgacg
     3541 gcggtgttgt acttgtttag cgagcgattt atgacctatg gcaaggctta tggcttatgc
     3601 gaatggttcg aggtagagca gtttcagaac ccctgaaatt tcagggagat gtcatggata
     3661 gggtggacag ttgcagccac tgcattaggg gataatcaga tgtccttcac tcgacgcaaa
     3721 tttgttctgg ggatgggaac agtaatcttt tttactggtt ctgcgtcttc tctgttagcg
     3781 aacacgaggc aagaaaagga agttcggtat gccatgattc atgacgagtc acggtgcaat
     3841 ggctgtaata tttgcgcccg cgcatgccgt aaaactaacc acgtccccgc tcagggaagc
     3901 cgtttatcga tagcacacat tccagtcacc gataacgaca acgaaacgca gtatcacttt
     3961 tttcgccagt catgtcagca ctgcgaagat gctccgtgca tcgacgtatg cccgacaggg
     4021 gcgtcgtggc gtgatgagca ggggatcgtg cgggtagaaa aatcgcagtg catcggctgc
     4081 agttattgca ttggcgcttg tccgtaccag gttcgctacc ttaaccctgt cactaaagtg
     4141 gcagataaat gtgatttctg cgctgagtcc cgactggcaa aaggctttcc gcccatttgc
     4201 gtcagcgcct gcccggaaca cgcattgatc tttggtcgcg aagatagccc agaaattcag
     4261 gcgtggttgc agcaaaataa atactatcaa tatcagctac ctggtgccgg gaaaccgcat
     4321 ctgtatcgcc ggttcggtca acatttgatt aaaaaggaaa atgtatgaac ccgtcgcaac
     4381 atgctgaaca gtttcagagc cagttagcga actatgtgcc acagttcact cccgaatttt
     4441 ggccggtgtg gttgatcatt gccggagtat tgctggttgg gatgtggttg gtgctggggc
     4501 tgcatgcctt gcttcgtgct cgtggcgtga agaaatcagt caccgattat ggtgagaaga
     4561 tttatcttta ctgcaaagcg gtcagattat ggcactggtc gaatgcgtta ctctttgtat
     4621 tgttgctggc aagtgggctg ataaatcact ttgcgctggt gggcgcaact gcggttaaaa
     4681 gtctggttgc ggtgcatgaa gtttgcggat ttttgttact cgcatgctgg ctcggctttg
     4741 tgctgatcaa tgccgttggg ggtaatggtc accactatcg cattcgtcgt caggggtggc
     4801 tggagcgagc ggcaaaacaa acgcgatttt atttgtttgg cattatgcag ggggaagaac
     4861 atcctttccc ggcaacaacc cagtctaaat ttaatccctt acagcaggtc gcctatgttg
     4921 gtgttatgta tggattgctg ccgttgttac tattgacggg gctgctgtgt ctctatccgc
     4981 aagccgtggg agatgtgttt cctggcgtaa gatactggtt attgcaggcg cattttgctc
     5041 tggcatttat aagcctcttt tttatcttcg gtcatcttta tctttgcacc acggggcgta
     5101 cgccacacga aacctttaaa agcatggtcg atggctatca ccggcactaa cgaatgatca
     5161 ttacacgagc agacctgcga gaatggcgta tcggagcggt gatgtatcgc tggttcctgc
     5221 gccatttccc acgcggcggt agttatgccg atattcatca tgcactgatt gaggaaggat
     5281 atacggactg ggcggagagt ctggttgagt atgcgtggaa aaaatggctg gcggatgaaa
     5341 acttcgcgca tcaggaagtt tcctctatgc aaaaactggc cacagacccc ggagagatac
     5401 cattttgcag tcagtttgcc cgcagcgacg atcatgctcg catcggctgt tgtgaggata
     5461 acgcccgaat cgcaaccgct gggtatgccg cgcaaattgc cagtatgggt tatagcgtgc
     5521 ggattggaag tgtcggcttt aacagtcaca ttggcagctc cggtgaacgc gcacgggttg
     5581 ctgtaaccgg taattcttcg cggataagca gcgctggaga tagtagtcgg attgcgaata
     5641 ccggaatgcg agtacgcgtt tgtacattgg gtgaacggtg tcatgtcgcc agtaatggtg
     5701 acctggtgca aatcgccagc tttggcgcaa acgccagaat tgccaacagc ggcgacaatg
     5761 tacacatcat tgccagcggt gaaaattcaa cggtagtgag cacgggcgtg gtcgactcaa
     5821 ttattctcgg gccaggggga agcgccgcac tggcatatca tgacggcgaa agagttcgct
     5881 tcgccgtcgc cattgaaggt gaaaacaata ttcgaaccgg agtccgctat cgtcttaatg
     5941 aacagcatca gtttgttgag tgctgatagc tttaccttta atcaaacgaa agacgccgac
     6001 gagcacggga tgcaggcttc acgaccgccc gagccatcgc ctcaccaatt tctgcacatg
     6061 ccgtaagtcc ctgaacgaaa ggctggtcgt gcatcaacca gggcaacgcg ccgaacgcta
     6121 cgcgtccacg aatatcttcg ggttgctgta acgtataatc ttcgccaata tcagggattt
     6181 catcccctgt tttctgtaat tgctcgcgta gcccagggaa aggaatatct ttcactttaa
     6241 gcggacgctg tccgcgggca tcaataaaaa cgtcaaacga gtagctgttg tcttccgttt
     6301 tcaggacggt gcgcgactca ttaatttcca ttttgtagtc ttcaccgagg gcgagaatat
     6361 gaatgattcc cgcttcacgt aaggcaagta ggcgacgaat agactctgaa gggattgccg
     6421 cataattatc gatgaaaacc ctggcaaggc ctttactgaa ccgtttatgg tcgtgttcat
     6481 tcagatgagg aacaatttcc tgtacggctt catgcaggcg cagaatgaca taacgccagg
     6541 gaacagtatg tttttctcgt ttattgcgtt cgacttcctg gagatttttt tctgcccagt
     6601 caaatgggtc gcgttgtttg cgctcggcaa accaggcttg agcaaaggaa tcgacattca
     6661 ggctctctaa ggctatgcgt tggctccagt ctggatcagc aaacttgatt tcctctacta
     6721 tcaatctaaa tacccgatcc aaaaggccct cttcaccttt ttgaatctca gcatttaatg
     6781 cctgatcggt gacgatgtgt aagggctcgt agggaatagg gcaatagaaa tcggcttcgg
     6841 gtaaaatacc cgtgcgcgac atcaacgtaa tatttagctt ttcacttgcg ttatcgcggt
     6901 taaaaacgac gtgttgttta tcatcttcaa tgaacgaacc atgctgaata gccactgcca
     6961 ttgccgcatc cagtccgctc aaggatgttc ccataatacc cacgttacac gcatcgacct
     7021 ttgcttccat caggcctgac cacggactgg gaaaatacgt tctggttgct tcttcttcat
     7081 caggccagac gtgacccgtg gcaatcaccg ctaaatcaaa cgtctcgctg ggtaaatcct
     7141 gatttgtagc gagcatgacg ccagcatttg taatttgcag atcggtaacc tggcatgatt
     7201 cataaacagc cactgcaaat ttttgctgtc gtgcctggtc tactaatcgt aaaaattgat
     7261 cgcggaaata ttcgcccagc agaattcgcg gtaaaaactg acgatcgtgc aaggtttctt
     7321 ttttaacgcc ataacgctgg agatggctgg cttcttgctt ttgtagccat tcgagatacg
     7381 tacaattaat cggcggtatt tcaatactgg caatatttgc cagcatcatt tttgagtttt
     7441 cctcatcact gtatggcatc ccgacaccgg cctcgtcagc ctgctcgaag atagaaatag
     7501 aaagtggagt ttgttgctgt agaagcgaga ataaggtgta gatccccgta ggcccggcac
     7561 ccacaatagc aatttttttc attgttactt acctgtacgc attctttcga atttagtgtt
     7621 atcagataag tttggttgag atttttagaa tttgagaaat ttcagacagg tatgaaaagg
     7681 gcgctaaccg cccttgatta aattctgtca ggcgagtttt gcctgattga tttgactcag
     7741 tggctcattg acatcgaaaa ctttggcgac aacttcctca acgccgagat ttttaagtcg
     7801 ggcagtatgt ttttccagat aggcaagggc gcttttttca tcagtaaaaa gatagatccc
     7861 accggcttcg tggttctttt cactttctgt ccataccttc cacagaaaac caggttcctg
     7921 attaatcgac tcagcaagtg gtttaagctg ctcagccatt gcgtcgccaa atgggccatt
     7981 aaaagcaaaa tgaagttgta acaaagttgc catagcaatt ctccttctga taacgcatac
     8041 gaagacagtg taaatctttt aacgtattgg atacagagaa gaaacagagg aaaaaaggag
     8101 aagaccagat taagaaaatc tggtgcgtcc gagtggactc gaaccaccga cccccaccat
     8161 gtcaaggtgg tgctctaacc aactgagcta cggacgcagg atggtgcgtt caattggact
     8221 cgaaccaacg acccccacca tgtcaaggtg gtgctctaac caactgagct atgaacgcaa
     8281 cgttgtaggt gacaacgggg acgaatatta gcggcagagt gggaaggtgg caagaggcaa
     8341 aacgtaattt tctgcgctat ttcgaccgtt tgcagagctt ttaagcaaat tggctatatt
     8401 ttgttgattt gcaagggcga tttttattca ggatcgcatt tacatctgat acaacccaat
     8461 aattcgtgta tcagagttta aacagagttg tttgccacca tctcaaatct gtcgtggata
     8521 aatcctccgt agcctttaca cttaggtagc aataaaggca cacagatgca gcaaaggaat
     8581 aaatcatggg atctgatgcg aaaaacttga tgagcgacgg gaatgtgcaa attgttaaga
     8641 ccggcgaggt cattggcgcg acgcaactta ctgaaggcga gttgattgtt gaagctggcg
     8701 gaagagccga aaataccgtg gtcacggggg ctggctggtt gaaagtggca accggtggga
     8761 tcgccaaatg cacacagtac ggtaacaatg gcacgctatc ggtcagcgac ggtgccattg
     8821 ccacagatat tgttcagtcc gagggaggcg caattagtct ctctacgctc gctacggtta
     8881 atggccgcca tcccgaaggt gaattcagcg ttgatcaggg ttatgcctgc ggtttgttgc
     8941 tggaaaatgg cggtaacctg cgtgtactgg aaggacatcg cgcggaaaaa atcattctcg
     9001 atcaagaggg cggcctgttg gtcaatggga caacctcagc tgtcgtggta gatgaaggtg
     9061 gtgaattgtt ggtgtatcca ggtggggaag ccagcaattg tgaaattaat cagggcggcg
     9121 tttttatgct ggccgggaaa gccagtgata cgttgcttgc tggtggcacc atgaataatc
     9181 tcggtggtga agactctgac actattgttg agaatggatc catttatcgt ctggggacgg
     9241 atggccttca gctctacagt tccggtaaga cgcaaaacct gtccgtgaat gtgggtggtc
     9301 gggctgaagt gcatgccggt acgctggaaa atgcggtaat acaaggtgga acagtgatcc
     9361 tgttgtcacc caccagcgcg gacgaaaatt ttgtcgtaga ggaagatcgc gcaccggttg
     9421 aactgaccgg gagtgttgca ttactggacg gcgcttcaat gattattggc tatggtgcag
     9481 atctgcaaca atcaacgatt actgtacagc agggcggtgt gttgattctc gacggcagta
     9541 cggtaaaagg tgacagtgtc acttttagtg ttggtaacat caatctgaat ggcggaaaac
     9601 tgtggctgat cactggtgcg gcaacgcatg tgcaactgaa agtgaaacgc ctgcgcggag
     9661 agggagcgat ttgcctgcaa accagtgcga aagaaatctc acctgacttc atcaatgtga
     9721 aaggggaagt taccggggat atacgcgttg agataacaga tgccagtcgg caaactctgt
     9781 gtaacgcact gaaactacag ccagacgaag acgggattgg cgcaacgctc cagcctgcgt
     9841 aaaaacacag gcgcaacccg tgaaggttgc gccttgtctt tattagcggg acgctcgttg
     9901 cagaatgatg gctgacggca gacgttgcag gaaccgcata cgcagcatca tcataatggc
     9961 tgccgacgtc aggccaataa taaagcctat ccagaagcct gctggcccca taggttcaac
    10021 gaccagatcg gtcagtgcca gaatatagcc gcttggcaag cccagcaccc agtaagccgt
    10081 aaaggtaata tagaaaatgg aacgcgtatc tttataacca cgcaaaatcc cactgccaat
    10141 cacctggatc gagtcagaaa tctgatatac cgccgccagc aacatcaaat gcgcagccag
    10201 cgttacaacc tcgggattgt cgttgtacaa cagggcgatt tgctcccgca gtgaaaccgt
    10261 gaatatggcc gtcagggttg ccatacagac acccaccata agcccggtcc gcgcagcggt
    10321 ttgcgcatcc agcgttgagc cctgacccag acgataacct acgcggatag ttaccgctgc
    10381 cgccagcgac atgggaagca cgaacattag tgaactaaag ttcagggcaa tctggtgtcc
    10441 tgcgacatca acaataccga gcggagacac taacagagcc acgacagcaa acagtgtcac
    10501 ttcaaagaac agtgccagcg caatcggcaa accgagttga atcagtcgtt tcataaccgc
    10561 aggatcgggt ttcgcggtgc ctttttcgtt acgaatatcg cgcatggagc gggcgcgttt
    10621 aatgtaagaa accatggcaa ggaacataac ccagtacacc gccgcagtag ccacgccaca
    10681 accaacgcca ccgagctcag gcataccgaa atgaccataa ataaagatat agttcaccgg
    10741 aatgttcacc agcaggccga taaagcccat taccataccc ggcttggttt ttgccagacc
    10801 ttcacactgg ttacgggcaa cctggaagaa cagatagccc ggcgcgcccc acaacaacgc
    10861 acgcagataa cccacggctt tgtccgccag agccggatcg atgttttcca tggagcggat
    10921 aatgtaacct gcattccaca gcaccagcat aatgagaacg gaaacaaaac ctgccagcca
    10981 gaaaccttgt cgcacctgat gcgcaatgcg ctcacgtcga ccggaaccat ttaattgcgc
    11041 gataaccggc gttaatgcca gcagcagtcc gtgaccaaag aggatcgccg gaagccagat
    11101 agaagtaccg atagcgaccg ccgccatgtc ggtggcacta tagccgcccg ccatcacggt
    11161 atcgacaaaa cccatcgcag tttgggcgat ttgcgcgaga atcaccggga ttgctaatgc
    11221 taataacaga cgcgcttcac tgatatactt ctgcacgtga acacctttta tttgtagtta
    11281 tatgagagac taaaaagccg cctgaatggg cagcaaaaag aagaagcagg ggaaattcag
    11341 tctattgtag cgaggattta ttaattctcc agtgaaaaaa tagtcattct taccccttca
    11401 ctggtaaggg cgtttgccac ctgctattgt gctgaacaga atgtcttaac tgatttcagg
    11461 agttgtaagt atgtttacgg ggattgtaca gggcaccgca aaactggtgt cgattgacga
    11521 gaaaccaaat tttcgtacgc atgtggtgga gttacccgac cacatgctgg acggcctgga
    11581 aaccggtgcg tccgtggcgc ataacggttg ctgcctgacc gtgacggaaa ttaacggcaa
    11641 ccatgtcagt tttgacctga tgaaagaaac gttacgcatt accaatcttg gcgatttaaa
    11701 agtgggggat tgggtaaacg ttgagcgtgc ggcgaaattc agtgatgaaa ttggcggaca
    11761 cttaatgtca ggtcatatta tgaccactgc tgaagtggcg aaaatattaa cctcagaaaa
    11821 taatcgccag atctggttta aagtccagga tagtcagttg atgaaatata ttctgtacaa
    11881 aggatttatt ggcatcgacg gtattagcct gaccgtcggc gaagtcacgc caacgcgttt
    11941 ttgcgtccat ttaattccgg aaacactgga acgcacgact cttgggaaga aaaaactcgg
    12001 cgcacgcgtc aacattgaaa ttgatccaca aactcaggca gtggtagata cggtagaacg
    12061 tgtgctggcg gcacgagaaa atgccatgaa tcaaccaggt acagaagcct gatctgaact
    12121 cgcccccgaa gagggggcga tagaatagcc tttagcgagc gactcgaagg ccgttttcaa
    12181 caccgcgtga gaacaccacc tgccagagct gaatatcacg ggcgcggaaa gcacctgcac
    12241 aagcattcag ataataggta aacattcgtt taaagcgttc actgtagtta tctgcaattt
    12301 ctggccatgc ggcgaggaat cgttcatacc acgccatcaa cgtagtatcg taatcagcac
    12361 cgaagttatg ccagtcttcc atcacaaagt ggggttcgct ggactgagca atctggcgta
    12421 cagagggcag gcaaccgttc ggaaaaatat atttattaat ccagggatca acattcagat
    12481 cggttttttt cgaaccgata gtatggagca ggaatatacc ttccggtttc aaattacgat
    12541 ccaccaccgc aaaataggta tcgtaatttt tcggtccgac gtgctcgaac atccccacag
    12601 aaacaatacg atcaaactgg tcgttcaggt cacgataatc ttgcagcaaa atggtgacat
    12661 ccaggccttc acagcgttcc tgagccattt tttgctgttc ggcagaaatg gtgacgccga
    12721 ccacgcttac gtcatagtta gatgccatgt agtgcgccag tccgccccag ccgcagccaa
    12781 tatccagtac gcgcatccct ggttttaact gcaatttttc acaaatcatt ttgagcttcg
    12841 cctgctgggc agattccaga ttatcggcat ctttccagta agcgcaggaa tattgcatga
    12901 agggatcaag catgcgactg aacaagtcat tacccaaatc gtaatgctct ttgccgacta
    12961 tccaggcacg ttttttactc tgcagattga agagacgggc gcctgcaata cgcagcgtgt
    13021 ctttgaaatg atgggggagt tggttctcga gacctgcgcg taagactttg ctaaaaaaca
    13081 tatccagtcg gtcacattcc caccagccat ccatataact ttcgcctaac cccaaagagc
    13141 cttcttgcag aacgcgttta aaaaaatcgg ggtttttcac acgaatatcc gccggggcag
    13201 aaccgttaat ggctataccg gcacggctaa gtaattcgtt ggcgatacgg taccagttgt
    13261 catccggtac actgacttct tctatacacg atgaactcat agtttctcca tcactggtcg
    13321 gtgatcagaa ccttaaaaca gcgtagacgc tttttgggct ttgtgagaaa tctcgcagaa
    13381 aaaaccgcga gtctataatc cgacgtagaa cagagggaaa ggaggaaccc ttgccgaaat
    13441 gaccatccat agtgagtcgg gaacgatcct gtccccgtaa aaacataata tttatcgtag
    13501 ctttaacaac tcgaaattag tataggccgc taatttcaat catacaacaa aattttgtta
    13561 gcagcctaag tataagtata tcgatataga tcagtgtgat tcgctatgag cgacttcggc
    13621 attgccatga ttctggcagc caacttcttc acaacgttgc atcatgtaac ccagcgcgac
    13681 cagcactact gttgataaca tcacgctggt ggtggtgagc aatggcgtgc tgatactgat
    13741 cagccaggaa actaccagac ttgcgaggaa gcacagaccc agttgcagag tgttctgcaa
    13801 cgctgcggcg cgaccggttg catgtgggaa gggacgcagc gcctgggcga caacaatagg
    13861 gtagatcgcg ccattggcaa tcgccatcac acagaatggg atcaggattt cgaccagcga
    13921 cacatggcta atgaagcccg cagcccaggt cgcaatgacg ctgacagcaa acagcaccag
    13981 caaccacggt aataactgct tgccttgcca tttctgcagt gcggcgcgac agccataacc
    14041 accaatcaga aacgcgatag tttgcgggac ataacttaaa ccaataactg ccgggctgta
    14101 gcccatttca ctaaggatga acggtgaacc ggtcagccat gcaaaaaaac tggctgaaca
    14161 ggctgcatat atcagcacgt tgccgcgata ggttttagaa cgtagcaggt cggtaaaggt
    14221 cagaccatcc tgactattgt tacgggcctt cgtcgtgggt ttgagccaga aaataggcag
    14281 aatcagcacc acggtaatgg caaacagggt ggcgaaaatc gcctgccagg aaaaatggac
    14341 cagcagccag cttcctaaca ggggagccag tgccggagac agacccacca gcggcatgat
    14401 ggtcgcaaaa atacggttga ctttctgtga aggataataa tctgtcacta atgcttgcca
    14461 gataaccgcc gcggcgcaga cccccacagc ctgtacaaaa cgcaatacca gcagcgtagc
    14521 ggcgttttct acccacagca tccccagact acctaacgca aaaattgtca ggccgattaa
    14581 taacaccggt ttacgaccat aacggtcgga gagtggcccc cacagaagct gggctgcggc
    14641 aaaaccggca aggaacagac taaggctggc actgacagca gacgcaggcg tttgcaggtc
    14701 ggcctgtatg gcggcgaaag caggcagata catatcggtt gccagaaaac cgagtacgct
    14761 caaacccgcc agccagacta aaaatctttt cccaggttgc atgtatattc tctaaatctc
    14821 tcaattaaac accgccgcaa agtgtaggga gtgaaattag tgttgtgaaa cggtaatatt
    14881 tgatgactgg tttcaaaaat tttgcaggca gactatgtgg tcagaatatt cactcgaagt
    14941 tgttgatgcg gtagcgcgta atggtagttt tagcgctgcg gcacaggagc tgcatcgcgt
    15001 tccttctgcg gtcagctata ccgtgcgtca gctggaagag tggctggcgg taccgctctt
    15061 tgaacgacgg caccgtgatg tggagctgac cgctgctgga gcgtggtttc tcaaagaagg
    15121 gcgctctgtt gtcaaaaaaa tgcagatcac ccgccagcaa tgtcagcaga tagcgaacgg
    15181 ctggcgcggt cagttagcta tcgcagtgga taatattgtc aggccagaac gtacacggca
    15241 gatgatcgtt gatttttatc gccattttga cgacgtcgaa cttctggtct ttcaggaagt
    15301 gtttaacggt gtctgggatg cgctttccga tgggcgcgtg gaactggcga ttggcgcaac
    15361 acgcgcgatt ccggtaggcg gtcgttatgc cttccgggat atggggatgc taagctggag
    15421 ttgcgttgtt gccagccacc acccgctggc gttgatggat ggcccgttca gcgatgatac
    15481 gttgcgcaac tggccgtcgt tggtgcgcga agacacctcg cgaacgctac ctaaacgtat
    15541 tacctggttg ctggataacc aaaaaagagt tgtcgtgcct gactgggaat catcggcaac
    15601 ctgtatctcg gcaggattat gcatagggat ggtgccaaca catttcgcca aaccgtggct
    15661 taatgagggg aagtgggtgg cattagaact ggaaaacccc tttccggatt cggcatgttg
    15721 tctgacatgg cagcaaaatg atatgtcgcc tgcgttaacc tggctgcttg agtatctcgg
    15781 cgatagtgag acgctgaata aggagtggct gcgggagccg gaagagactc ccgcaacggg
    15841 tgattaacga cgatagtcgc ggaacgggcc gtcagccacg gagcggcgtt caatcaagcg
    15901 cggatgcact tcaatggact gcggttcttc acgtttgttg acgatacgat ccaacagcat
    15961 gttgaacgct gtttcaccca gcgaatcttt tggctggtgg atcgtggtca gcgccggcgt
    16021 aaaatagcgg gcgttgcgca cgttatcata accgatcagc gaaacatcct gcgggacgcg
    16081 cagacccatt tcatcagcag cacaaagtgc gcccattgcc atgatatcgc caccacagaa
    16141 gacggcagta gggcgatgcg gctgcgacag tatttgctgc atggcgcgat aaccagattc
    16201 gggttcaaag tcaccctgaa caatccagct ttccggcacc ttgatcatcg cttcttccat
    16261 cgccttcata aaaccggcaa ggcggcctgc gccggtgtta cgttccagcg ggccggggat
    16321 gacgccgatt tcgcggtgac cgcgttcaat cagataacgc ccggccatgt agccgccttc
    16381 gaacgcgtta tcaatgaccg catcggtgaa gtcagctttt gcttcacccc agtccatcac
    16441 caccattggg atatggcgat actcttccag catcgccagc aacggctctg ggtactcaga
    16501 acacatcacc agcagaccat cgacgcgttt ttgcgccatc atcgacagat aagcccgctg
    16561 tttctcaaga ttgttccacg cattacccag aatcagggtg taacctttct ggaagcaatt
    16621 tttttcaacc gcttcaatga tctcggcaaa ataggccgct tcgctgctgg tcgccagcaa
    16681 accgatagac ttggtgtggt taaccttcag gctacgcgcc accgcgctag gggagtagtg
    16741 taattcttta atcgctgccc acacggcgtt gcgcgtttct tcagcgacga aacgtgtttt
    16801 gttgattacg tgtgacacag ttgtagtgga aacgtttgct cgtttcgcta catcttttat
    16861 tgttgccatt ccatttcact ccagacccta acttcagctc ctgcaaaatc gcaaggtaaa
    16921 cgtttgcctt tacacacctt acgcacaaca aaagtgttgc ggtacgccgg aaaaacgaca
    16981 cggagcgtca ggaaggggtc aatggccagt acgcaaatag tgtaagcgtg gaattttggc
    17041 cgatcttgac gaaaagggga agtggtaaaa acacttatca ttataaatga cgggagattt
    17101 tttcatcaca gtgtgtaaaa atgcgtaata tcactatttg tgtggtaata aaaaaggaga
    17161 aaacttgatg agcaccgacc ttaaattttc actggtaaca acgattatcg tcctcggttt
    17221 gatcgtggcc gtgggtttga ctgccgcgct gcactgattt cctctcgagg gagcgcgttc
    17281 tccctcgcat ctccttcctg tttgttagca aaatggcaaa agtgttttct ctttttgtta
    17341 ttttcatttt tttgtgattg ctgtcatgct ttcgccactt taatgaatat cgcgtaatga
    17401 cgcgaagagg tggagataag gcatgaaaat taactatccg ttgctggcgc tggcgattgg
    17461 cgcgtttggt atcgggacaa cggagttctc tccaatgggt ttgttgcccg tcattgcgcg
    17521 cggtgtggat gtctcgattc ccgctgccgg aatgttaatc agtgcctatg cagttggcgt
    17581 aatggttggc gcgccgctga tgacgcttct actttctcat cgtgcccgcc gcagtgcgtt
    17641 gattttcctg atggcaattt tcacgctcgg caacgtactt tccgccatcg cgccggatta
    17701 tatgaccctg atgctttcac gcattttgac cagcctgaat cacggagcat tttttggttt
    17761 gggttcagtc gtggccgcaa gcgtggtgcc aaaacataaa caggccagcg cagttgccac
    17821 tatgtttatg gggttaaccc tggcaaatat cggtggcgtg ccggcggcga cctggttggg
    17881 tgaaaccatc ggctggcgga tgtcatttct ggcaacggcg gggctgggag tgatttcaat
    17941 ggtaagtctg ttcttctcat tacctaaagg tggtgcaggg gcacgacctg aagtgaaaaa
    18001 agagctggcg gtattaatgc gtccgcaggt gctgtctgca ttgctgacga cggtactggg
    18061 agctggtgca atgtttactc tctacaccta tatctctccg gtactacaaa gtattaccca
    18121 cgcaacacca gtgttcgtca cggcaatgct ggtgctgatt ggtgtcggat tctctatcgg
    18181 taactatctc ggcggcaaac tggcagatcg ttcagttaac ggcacgttga aaggcttttt
    18241 gttgttgttg atggtgatta tgctggcaat cccgttcctg gcccgcaatg agttcggcgc
    18301 agctattagc atggtggtgt ggggcgcagc aacctttgcg gtcgtaccgc cgttacagat
    18361 gcgcgtgatg cgtgtcgcca gtgaagcgcc aggtctgtct tcatcagtca atattggtgc
    18421 ctttaatctt ggaaatgcgc tgggagcagc tgctggtggt gcggtaattt ccgctgggct
    18481 gggatacagc tttgtgccgg tgatgggagc gattgtcgcg ggactggcat tattgctggt
    18541 gtttatgtca gccagaaaac aacctgaaac agtttgcgtt gctaacagct aattaaacaa
    18601 aaacgcagaa ggataacctt ctgcgttaca gttgctgaca acgtgcgatt tatcttgccg
    18661 aatgcgctgt taacgcctca tccgcgttgg catggtaagc gaagcgcatc aggcaatgct
    18721 gcatttgcca tcagttatta tgcagcgaga tttttcgcta cgaattccca gttcaccagc
    18781 gcccagaagt gctccagata gccaggacgt gcattgcgat agtcgatgta ataagcgtgt
    18841 tcccagacat caacggtcag cagcggagtc gcatcggtgg tcagcggagt acccgcgtta
    18901 gaggttgaaa cgatagccag tttgccatcg ctgtttttca ccagccaggt ccagccagaa
    18961 ccaaagtttt tgatcgctgc atcagtaaac tgcgctttga aatcggcaaa gctaccaaaa
    19021 gatgcggcga tagcttcagc gacttttcca gtcggttcgc caccggcgtt cggtgccagg
    19081 cagttccagt agaaagtatg gttccagacc tgagctgcgt tgttgaatac gccaccttca
    19141 gagctgcgaa taatctcttc cagtgattta ccttcaaacg cggtaccttt aatcaggttg
    19201 ttcaggttag tgacataagt ctgatgatgc ttgccgtagt gatactcgat agtttccgca
    19261 gaaatgtgcg gtgccagagc atctttagca tatggtagtg caggtaattc gaatgacatt
    19321 gctactctcc tttattatta atttgcatac gtacaatagc cttattgtgc gtatggatag
    19381 ggtagcaaat taaaaggtga gacaaaagat gaacttaccc tgttgccgta acaacagggt
    19441 aaagttttag cgaagtgttt ttggggtcat tacccgacga gcgccaacat agtggcgctg
    19501 ccagtagtct tcactgagag aagtgatttg aatttcctga cctgtgcgcg gtgactgaat
    19561 aaatttgccg ttgccgacat acacgccgac atgatcggct gtgccgcgtc cctgagtacg
    19621 gaaaaagacc aggtcgccgt ttttcagttc actacgttcg attggcgctg catcacgcag
    19681 gtgatacatt tcattcgccg tacgcggaat acgaattttc accaaatctt tataagcgta
    19741 ataaaccagg ccgctgcaat caaaaccggt acgcggtgag ctgccacccc aacgatatgg
    19801 cttaccaatt tgctgcatca gtttattcat tgccactttc gtagcttttt gcaccttcgc
    19861 tttgtgcgcg tcggcaagag ttcctgacgc ggcatttttg actttcacac aatgcgattt
    19921 atagccctta cgtttggtac acttttcggt aaagctggct gaggccgttt tattgacggc
    19981 gtgtttgctg cgagttttgg cgtttttaga agcggtaatg gaagattttt taacggaaga
    20041 agcggttttg cttttggtcg tacttttact ggcggctttt ttcgctgttt tctggctggc
    20101 tttttgggtc tttttggtgg ttgccgtgct tttcttttta tctgcttttt tggtgatatg
    20161 agtggtagca gaactctccc tggcttgctt tgaagcatgg gccataggcg ttaaaggcag
    20221 ggtggtaaaa agtaaagcac agagcgtgat cgaaatacgg tttatccgcg ccactgagcg
    20281 tccctctgtt aaggcaggtc tatcatgcct gcgtctgatt tacaaaactt gatgattcta
    20341 atgcatagaa gaagagaaag ttaatagctt ttattgttaa aaaatacgtt tcgttggcga
    20401 gcgcaaaaaa atgcagcagt gaaagctaaa aatggttata aatacgccag tttgccgagc
    20461 atctgggaag aaacgaatat ttaaaatgca acaaatagag caaagggata aaatagcaaa
    20521 agcgtgatga aaatgttatt ttccgatgcc gcactgaccc gctacaatgt cagattactg
    20581 ccgtaaagaa gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa tccaacgcca
    20641 gattgctgaa aacccgatcc tgctgtacat gaaaggttca ccgaaactgc cgagctgcgg
    20701 tttctctgcc caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg cctatgttga
    20761 tattctgcag aacccggaca ttcgtgcgga actgcctaaa tatgctaact ggccgacctt
    20821 cccgcaactg tgggttgacg gcgagctggt aggcggttgt gatatcgtga tcgaaatgta
    20881 tcagcgtggc gaactgcagc agctgatcaa agaaactgcc gctaaataca agtctgaaga
    20941 gccggacgcg gaataatccg tcctgcaata ctgacggatg cgaacgggca tccgtcagtc
    21001 ctttctctat ccccaatcca gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag
    21061 cgtaaacatc ggcgtttgcc ggaccggtag atcattcact tctgttagcg taaagcgcag
    21121 ccgtggttcg cgacgcagtg cggtagtcag cgcgtggaaa acctctgggg cgagtaattc
    21181 ctctttttcc tgccagacca gcattttttt gccaccttgc gccagataga gtaacaattt
    21241 gccgccagaa acgaccacca gcgcacccgc ccgacgcgtt ggaaccagcg ttgccggatg
    21301 tgcaggccag ggcagaagat ttccccacac attagccgga tcgttggcag agagcgccac
    21361 tggtgtatag tggcgcgttt gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt
    21421 cagacgttca gcgaattgcg cgccacccag accttctaca aaacgacctc gcataattcg
    21481 cccggagtct tccatacttc gacaaagcgt ttgcatcgat ggaaacccgt cagggatatt
    21541 ttcggctatc accgcctgac gactgatgat gccgtagcgg tcgagcatat tttccgccag
    21601 cgccagcatc ctttcggtat cgtttagtgg ctccacctgc aataacgacc agcgtccagc
    21661 cagatttggt gtgttgtaag ataccagcgg cgagacgggt tgcgcataga caggacgtcc
    21721 acgacgagcc cggtgactgc ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag
    21781 ggcgcgtaac ggtgcccaaa tgtcgctggt gatgacgcct tgccagacca gcgcccataa
    21841 cgcctcttgc agggcagaga gatcaaccga ttcgccgatt ttgtcgcgta tccgttggct
    21901 gatttgttgt gcaaaccagg ctcctccgtc agccagaaca gcgattatcg cttgctgcag
    21961 cgccgaacga ttcgcctgat ccgcttcggc gggagtgaac gattctgcgg catattcctg
    22021 tagatgcagt gccaccaggc cgtcatcttc acccagcttt ttttgtcccg accagataac
    22081 cgctccggtt gccagtaatt catcgagcat ttctggcgaa tagtcgcgta cgcgggcagg
    22141 caggatctgg ctttcccaga gtgaggcggg taaaccgact ccggcaagct gttcgatcac
    22201 ccgcatcacg ccatctacgc cctcataaac gcctggcgat gttgaggcaa agagcgccgg
    22261 gctaccatcg gtggcgggta atacgccctg acgttccagc agcaatcgcg cataggtcgt
    22321 ggctgcaacg ggacgcgtcg cttctctggc ggcttgcagc gagcgcaaac gcagacgacg
    22381 aaatacttca tcgctcaccc agatgtcttg ttgcagattc atcaccagac cctgttcacg
    22441 cagttgctga agctgctctt cgacaatggc aataccgaga ctaaactcat gagccagttg
    22501 ttcagccgtg accagagcat gagcccggag atagcgcaga aagaggtcgc gaagcgggta
    22561 actgactcta tgtaaataaa tctctggcaa tgactctggt agtcgtacgc cgagggcatc
    22621 acgcagcctg gcggcatcat ccatacaggc aagacgctcc tgtccgctaa tcatcgctgg
    22681 gaagattcgt tttactgcaa gaagattttc cagatacgac gcaacctctt cactgctgcc
    22741 tgtatgccgt tgcgccaggt cttcaacggt cattggcccc agttcgcgca gcaggtcgaa
    22801 caatccttct tcacctttcg ctcttctgcc aggagccagt cgttgcaact cttcttccac
    22861 ctggcgaatg acctgcgggt cgagtaattc ccccggatcg acctgtccga gtagattgcg
    22921 cagtaactcg ctgtccagcg acagtacgga tgcccggcgc tctgccagcg gggcgtcgct
    22981 ctggtacata aattccgcga catagccgaa caataaactt gtggcgaaag gcgagggcgt
    23041 agtggtcgtt acatcggata tttgaatttc gccaccgttc aggcgacgca tcaaacgttc
    23101 cagtgcggga agatcataaa catcttgcag acattcgcgt agggtttcga gaatgaccgg
    23161 aaaatccgga tatccctgag cgatttccag caactgactg gcgcgcagcc gttgttgcca
    23221 aagcggggtg cgatggcccg gagtgcgccc cggcattaat aatgcccgcg cggtgcattc
    23281 gcgaaaacgg gcggcgaaaa gtgccgagct gcctaccgcc tcgcggacaa tttgcagcaa
    23341 cttttctggt tcaaacaaaa aaatcgcggc gtcgggcaat ttaccatcag tgtcaggaat
    23401 gcgcgcaaca atgccgtcat cactggcgac cacagacgcg tcagcgcccc atagggcatg
    23461 tattcgcccg gcaatcgcca gcgcccaggg ttcatgcacc cgtcttccat agggagagtg
    23521 caaaataata cgccagtcgc caatttcatc acggcaccgt tcgaggacca gatggcgact
    23581 ccccggaacg atgcccgtcg cgttgcgttg ctcgtcaatc aacccctgaa tattggcgtt
    23641 cgtattttct tctgccagcc acgggggaat agtcccggaa aagaacgcgc catccgccag
    23701 taaatgaaga aaatcgccga tcatctcgcc taattcagcc ggacgtccgt taccttcacc
    23761 acgccagaag gggagccggg cagaacgacc cggagcagga gtcacaatca cctgatcgcg
    23821 ggtgatttgc tggatccgcc atgaggtagc gccgagagtg ataatgtcgc tcacccgcga
    23881 ctcatatacc atctcctcat ccagttcacc cacccgccgc gaaccggcct gttcttcacc
    23941 ttcgggtaat aacacgctat acatgccacg atccggaatg gtgccgccgc tggtaaccgc
    24001 caacaattga gcgccaggtc gggcggtcaa tatcccggtc tccctgttcc agaccagttt
    24061 ggggcgaaaa gcagaaaaat cgccagaggg atagcgcccg gaaagcatat ccagcgtggc
    24121 gtcaaaaaca cgtcttggca gatctttcca cggtgcggca cggcgtacgc gggagtacca
    24181 ttcgtctacc tgtaatgcat ccatcgccgc ggcggcaacg gtttgctgcg caaggacgtc
    24241 gagtggatta tgcggcggtg tcaggttttc cagcctgcct gcgaacatac actctacaat
    24301 gactgcggaa tcgactaagt cacgccgggt acgagggaaa aacagccctt tagatacacc
    24361 gccaacctga tgtccggcgc gaccaatgcg ttgtaaccca ctggcaacag aaagcggcgt
    24421 tgccacctga atcaccagat ccaccgcgcc catatcaatc cccagttcga gactggaggt
    24481 tgcgaccacg cagcgtaatt cacccgattt cagcgcctgt tcggtgattg ctcgttgttc
    24541 tttagagacg gagccgtggt gtgagcgggc aataaaaacg tcgctacttt gtacatggtt
    24601 agaggttgcg ccggaggtcg actcgaaatg ggccgtatca acggcgatag acggggaacg
    24661 ctgtaagcgt gcggcgtaaa gctcatttaa tcgtgccgtc agtttttccg ccagcccacg
    24721 tgaattagta aagacaatgg tcgagcgatg gcgcaacact tcatcaagga tacccgtttc
    24781 aatatatggc cagatggagc cttcccggcc ggcatggctg tcttcgccgg tgccgctggc
    24841 gaccgatgag acatcatcca tattggcgac cggtacgaca attcgtatct gcggatggcg
    24901 cattgcgggc gggttgacta ccgtaaccgg gcgatcgcca ccaagaaatg ctgccacatc
    24961 gctggctgag cgcacagtgg cagaaaggcc aattcgctgt gctgaggtgt ggagcagcgc
    25021 atcgagccgc tccagactta acgccagatg cgcaccacgt ttactgcccg ctaccgcgtg
    25081 gacttcatca ataattaccg tttcgacgcc gcgtagcgtt tcgcgcgcgc gggaggtcag
    25141 catcagatag agtgattcgg gtgtggtgat cagaatatcc ggcggattac gggtgagttt
    25201 gctgcattcc tgtgcaggcg tatcgccagt acggatccct acgcgaagat tgacttccgt
    25261 ttcgccgcgc cgccgccgtt catcggcaat acccttcaac gggagctgca agttgcgctg
    25321 aacgtcggtg cccagagctt ttatcggtga aatatagagg atgcgtgagg ttttacgctt
    25381 atgcgcctcg cgggtatctt cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa
    25441 tgctgccagc gttttcccgg agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc
    25501 cgccacatgc caggtttgcg actggacagc ggtcggctgt ttaaaggcgc gaagaaacca
    25561 gtcgcgggtc gccggtgaaa acacgtccgg caggagcgat gaagggtctg gattatctgc
    25621 cattaacgct attccccgtg aatatcatgc ttgatgcgtg gaatagcctc actatagatg
    25681 cctgaatcat tgcatgtcat caggcatcga ctcgattaca cctcttcggc ggcaggtagc
    25741 ggccagcctc ccagacgttt ccagcggttg acgatttcac aaaacagcac agcagtgcgt
    25801 tcggtgtcgt acagcgcgga gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg
    25861 caagcctttg acaatacggt ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa
    25921 gtggcgaaag ggtggaacgg gttacgtttc agtgaggcgc gttctgcggc ggccatcata
    25981 aagctgtgat caaaattggc attgtgcgcc accataatgg cgcggttaca gccgctcgct
    26041 ttaatacctt tacgtacaac tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg
    26101 ccgcgatcgg gatcgttcgg gtcaatgccg ttgaaggcga gggcctctgg ctgcaaattt
    26161 gcgccgacaa atggttcgac gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt
    26221 tcatccattt tcagggtgat ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat
    26281 ccggctgttt caacatcgat cacaacagga taaaaaccac gaaaacggtc gcacagaccg
    26341 gtaagttgag cgttatcgga catctgattc tcttaatacg gataaattgc agcgcgcatt
    26401 atgacaaata ttacttgtcg atgcagtaaa gatgcgggcg cgatgagttc acgcccggca
    26461 ggagattagt tgcccagacc gcgaccggcg tctttctctt cgattaactc aattttgtaa
    26521 ccgtccggat cttccacaaa cgcgataacc gtagtaccgc cttttaccgg acccgcttca
    26581 cgggtcacgt tacccccgtt ttgacggatt ttttcgcacg cttcagcggc gttatctacg
    26641 ctaagcgcga tgtgaccata agcagtgccg agttcgtatt tatccacgcc ccagttgtag
    26701 gtcagttcaa tcaccgcttc ttcggtttcc gggccgtagc caacaaacgc cagtgagtat
    26761 ttgtattccg ggttttcgct ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa
    26821 tcgatggagc gttgcagatc gccaacgcgc agcatggtat gaagaagacg catcttttta
    26881 tcctcaaaat ggctaacaga ttcaaaatgt tgttttagta tagcggcggc tttacgccgc
    26941 tcgcaatgtt ggattacaac gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga
    27001 aactttcggc acgctgtggg ttaagctcag ctttgcgctg caagcgggcg accagatccg
    27061 ggttcgcaat ccagtcacga ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg
    27121 tttcagcttt ttctactgtg tatgcacctg cgccgataat cggaccgtgg aaacgggcgc
    27181 gtactttttc gcggaacgca tcagtatacg gttcaccccc cgcccaatct ggttctgaca
    27241 tatgcagata agcaatgccg cgtttaccca gttgttcaat cagatacagt gcatcggctt
    27301 cttcattcgc gccgttatcc gtgttctgga aagtaccgat tggcgaaacg cgaatgccaa
    27361 tgcgatcggc accccattct tcaatcccgg catcgaccac ttccagtacc agacgtgcgc
    27421 gattttccac gctgccgccg tactgatcgg tacgatggtt tgaagaagga gaaaggaact
    27481 gatgcagcaa ataaccgtga gcagagtgga gctctaccag atcaaaaccg gcttcacgcg
    27541 cgttagcaat ggcctgacgg aaatcattga cgatacctgg aatctcttcc agttcaagcg
    27601 cacgcggcat ggatgtttca acacggatcg cctgaccatt ttcatcgcgc agagaagtac
    27661 gtgttcccgc gctaagtgct gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg
    27721 cgtgagaaat gcgtccggtg tgccacagct gcacggccat atgaccattt tcagcatgaa
    27781 cgccagcggt gatttttttc catgcggcaa tttgctccgg actatggatg ccaggcgcac
    27841 ctgcatatcc ttttgcctgg gcagaaattt gcgtggcttc actaataatc aaaccggcac
    27901 tggcacgttg gcgatagtat tccgccatca acggggtagg aatgtcaccc ggttcaatac
    27961 tgcgcagacg cgtcagcggt gccataaaaa tacggtttgc cgccgtgatc gcgcccactt
    28021 tcagtgggga atacagtttt tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa
    28081 atgcctgcta aacggcaggc gtcgcaataa tgttttttac atgggccagc gcgttttcca
    28141 gtggctcgaa actgcgcgaa attttggcct gcagattcgc acccagccat agtgcgtaaa
    28201 gcacttgtgc ctgttgtagc ggttcgccac aaaaggttaa acaatggttc tcacggccat
    28261 tttccagcgc ctgagagagc agggcgatca cgccgcgagc acctttatcc atcgcgctgc
    28321 gcatatcttc tgacagatcg cacacttcgg cagagagttt tactgtcagg caaccactga
    28381 tggttccatg ttggcaaaac tggttcagtg tttgctggta ataagccagt atgcggtcgc
    28441 ggtagttacc ttcgccggat tgcagcaact cagtcagtcg ctggtgatat gcggcgtaat
    28501 gacgctcaag catggcaacg ccaaacgctt ctttagagcg aaagtagtga tagaaggacc
    28561 ctttcggcac ttcagcggtt tttagtaatt cgcttagccc catcccggtg aatccacgtt
    28621 gcaggcaaag ttgctcgccc gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt
    28681 tgttcatgcg ttggagtgta gtagaccagt cggtctagtg caagcaaagt tgctactttg
    28741 gacattaaca gcttcaataa tggtaataat cttatctgag gagggcgaag tggcggagca
    28801 attagagttc tttcccgtcc agagcccgtg ccggggaatt tgccagtctg acgaacgcgg
    28861 gttttgtcgc ggctgttttc gtagtcgtga tgagcgtttt aactggaata aaatgagcga
    28921 tggcgaaaaa caagaggtgc tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc
    28981 aaataagcca gcgtcatcag acgaaccgga gcaaccatca ctcttttgaa cctaatcttg
    29041 cgtatactcc tctcatatct gataagagga agtgattatg gttcagcgta ttactattgc
    29101 gccgcaaggc ccggagtttt cccgttttgt gatgggctac tggcgattga tggactggaa
    29161 tatgtccgcc cgccagctgg tcagttttat tgaagagcat ctggatctcg gcgtgaccac
    29221 cgtggaccat gctgatattt atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact
    29281 gaaactggca cctcacctgc gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac
    29341 gaccgcgcgt gaagaaaacg tcattggtca ttacatcact gaccgcgatc acatcattaa
    29401 gagcgccgaa cagtcgctaa ttaatctcgc gaccgatcat ctggatctgc tgttaatcca
    29461 ccgaccagac ccgttaatgg atgccgatga agtggcggac gcgttcaaac atctgcatca
    29521 gagcggcaaa gtgcgtcatt ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt
    29581 gcaatcacgt ctgccgttta cccttgccac taatcaggtg gaaatatccc cggtgcatca
    29641 gccgttactg ctggatggca cgctcgacca actacaacaa ctgcgtgttc gtccgatggc
    29701 gtggtcctgc cttggtggtg gtcgtctgtt taatgatgat tatttccagc agctgcgtga
    29761 tgaactggct gtggtggcag aggagttaaa cgcgggctcg attgaacagg tggtttacgc
    29821 ctgggtatta cgtttaccat cgcagccgct gccaattatc ggttcaggta aaattgagcg
    29881 cgtacgggca gctgtcgaag cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat
    29941 ccgtaaagcg gcactggggt acgacgtacc gtaagcactt ttaggaatag ccgccgttca
    30001 aaaatgtgtc actggtttac acttattcag gaatgcacaa tgaacggagg tcctatgaaa
    30061 cgttttagtc tggctattct ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa
    30121 aaagtcgaga tgaacctcgt cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc
    30181 attactgaaa ccgataaagg tctggagttt tcgcccgatc tgaaagcatt accccccggt
    30241 gaacatggct tccatattca tgccaaagga agctgccagc cagccaccaa agatggcaaa
    30301 gccagcgccg cggaatccgc aggcgggcat cttgatccac aaaataccgg taaacatgaa
    30361 gggccagaag gtgccgggca tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc
    30421 aaagctaccg atgccgtcat cgcgcctcgt ctgaaatcac tggatgaaat caaagacaaa
    30481 gcgctgatgg tccacgttgg cggcgataat atgtccgatc aacctaaacc gctgggcggt
    30541 ggcggtgaac gctatgcctg tggtgtaatt aagtaattaa gaggccagcg taccttgcgg
    30601 tggtgcttgc tcaagttgcg aaagcgagca gtacagccgc cagacaattg ccgccagctc
    30661 acgggcggca ggttgatgat gacgggcaag gctgtcgcaa atccgctgta attcttcaag
    30721 tgtggccgcc agtgattttt gctgaacgcc acgctcactc atcactcccc gcaacagtga
    30781 aatacagtta tcccgtactc gcgataacgg atcggaacgc gattcccagt cgcgcaattg
    30841 ccagacaaca tgagaacagt tcagcagcac tacaccccag cgtaataacc aacggcgagc
    30901 cagcgcatcc tggctgttac tcaactgact gacgtgatga taagtgagcg attcaaattc
    30961 gctttcactc agtgttggat ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg
    31021 aatatggcgg cggcttttac gagcatccga tcctggacgc agaatggcga acgctagcca
    31081 agccagcgca acgccaacga tttttgccag attatcgtta agaaaatcag caaaatcata
    31141 caccggggga ttagtgacag cgataaacga acccataaaa acaatcagtt gcccccacag
    31201 tgcggcaaat ttgggcatct gcaatttaag aagctgcatt gtcgccagca gtggaaagag
    31261 aaacagtaaa aattgccaca gatcgctaat ctggaccatc aggccaaatt tgaccacaaa
    31321 gctgaatagc gaaagtaaca ccagcgtgcg catcagcagt gacaacgact taaacggtgc
    31381 tgcgacggcg gagtagagta cgcagctaat tgctgccagc gttaatgcat tggcaccggc
    31441 atcccattgc gatgcaatac tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag
    31501 cccgctccac atagcttcgg cgttgtcggt atgacgagcc agagcggatg tccgtgcgag
    31561 tctggcatga tcatctacac cgctttgcag acgatgtaat tcctgactac tttgcagata
    31621 aaggcggcaa aaataacgta gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt
    31681 ggtcgggcgt agcggggcga taatacgtgc gacggtgtaa gcatctgttt gcgaactggc
    31741 gagcgccgtc agcaattgtt cgagaatttc tcgcgtggca cctggcggtg agggccagtt
    31801 gagcaacata cgtcgcaggc tggagatgac actggtcata cgtaattgct ggtggagtag
    31861 cgcattaagg cgcgcgtttt gctggcgaaa acgatagtgg ctccagaaag cctggatacg
    31921 cagcaaattc atggtcagta tctgcccaat caccccctca tgtgctgcac gaatggcatc
    31981 gtttgtttca ggctgccaga gtaaactggc atgttccagt aatcgggcgt gcatgttttt
    32041 caatgcggtt aaaagggctg tagcatcgga actgctaggc aggatcatca tcatcatgcc
    32101 gccgcacaaa ataccgacta tcacctcgca aacgcgcgcc tgagcgatat cccacagctg
    32161 gctggcctca gtaatattaa ccatcggaaa ggcgatgatg gcagccgtgt agcccgccag
    32221 ttgaaatgca tacgcgacgt tattcgtgaa gtgcgcacag gcccaggtac aaaagccaag
    32281 ccacgccgac atgctcaata gaaaaaacca cggctcattg agcgtatgcc cggcaagaag
    32341 cagtgccgca atggctccca gtaaactgcc agcgatgcgt ccgaggcttt tgctgatgac
    32401 accgccaacg gtgggaaagc taaccactgc agccgaggtc atcgcccaat agggttcatc
    32461 cagattcaaa taataggcaa ccgtcagcgc cagacacatg gcaatggtat tgcgtaacgc
    32521 ataacgccat tgcgccagcg tggccttgaa ccagggcaaa ttgcgcaagg accatgacga
    32581 tgcgttcatt attgacctac ggcaatgcta caggtggtgc ccgacaccag cgtgacatcg
    32641 cctggaactt tatccagcgc aaagcggacg ggaacgcgtt gggcgagacg aacccagggg
    32701 acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctttg atcataaatc
    32761 gcccgaccga tactggaaac gtgaccccgt aacgttttgt tatcactata gagggtaatt
    32821 tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata accaatgacg
    32881 taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc gtaatcgccg
    32941 atgcgggtag tgaggttagt cacccaacca ctcaccgggg cgcgaatttc cgtttgcgcc
    33001 agttgccatt gtgtctgttt cagcgtggcc tgtgcggcat taacactggc ctgcatcgct
    33061 ttaacattga ggttagcggt atcgagttct tcggcagaaa taaaattttg tgagagatga
    33121 cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt cgccagctga
    33181 gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag cagaagatcc
    33241 cccgcgttaa ccagttggtt gtcttttata ttcagctcaa caatacgtcc cgacacctgt
    33301 ggggtgatag aaaccttttc tgcgcgtatt ttcccgtcgc gtgtccacgg agattgcatg
    33361 taataattcc acagccacca tccggcaaga accgcaacta tcgctacaat gattgttgag
    33421 aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc agagcaaggc
    33481 aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg tcaccggcgt
    33541 agatccagcc acgaagcagg cggtgtacga caagccagat gacgaatcca aacgcgaagg
    33601 ctttgaaaaa cggaggaaaa tagacggacg caccgaacac cagatcttgt aagggcaatc
    33661 ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat atattgcgtt
    33721 agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa aaatatgtct
    33781 gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc acactattct
    33841 aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga atcgccacta
    33901 ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca tcgcctgaaa
    33961 ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca gttacctcca
    34021 gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc actggtccgt
    34081 actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc cagcgatcgt
    34141 cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga aatggaagct
    34201 gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga actggagcaa
    34261 ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc caaagggtga
    34321 aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag cgcggagaaa
    34381 cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag aaacgagtgt
    34441 tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt tccagctcga
    34501 caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca cctgcaacag
    34561 cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc cccaggaaac
    34621 caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg ccctgaatct
    34681 gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt ttcgcttcag
    34741 aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca agagacagac
    34801 ctaccattga aacaaccaat acgcgtttaa tcattgaaaa atctcctgtt caccatgaaa
    34861 cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca tggttacatg
    34921 ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata aacaaaacat
    34981 tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa tcggtcattc
    35041 cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc aaacgtcaga ttgaaaaagg
    35101 atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc ttgatggtgt
    35161 tgatgttgtg ttggcgacaa tagatgaaca ccgggtcgca cagctggcaa gcttgagctg
    35221 gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc agcagcttac
    35281 actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg atgcggtcaa
    35341 tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg gctgtcacgg
    35401 tcaaaccgtc tggcatgaac cgacgggcgt ggaaccacac actttacaga ttggcgataa
    35461 caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc gtcgcgatat
    35521 tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct tgctggctca
    35581 cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt cactgctcat
    35641 tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga tggatgcctg
    35701 gatctggcgt caggccggta aaccttacga taaagatgcc gagtgggcac gggcgggtaa
    35761 agttattctc ccactgctgc aaaatatgct cagcgaccca tatttctcgc aacctgcacc
    35821 gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt tacgccattt
    35881 tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg ccgtgaccat
    35941 ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg gtggaggtag
    36001 tcgtaatccg ctactcatgg cgcgtctggc ggcattactg ccaggcacag aagtcaccac
    36061 caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg cctggcttgc
    36121 ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg caagccagga
    36181 gacggtactg ggggctattt tccctgctaa cccgtgatat cagagttaac tgaatttttc
    36241 ttaccgcgtc tgccgttaca cttggaacgt tagggagggc gtattgccct ccagaccagg
    36301 aaagtcttcg ggaaatgtct atgaaaaaac tgttactgat ctgtttgccg gtgcttctca
    36361 ccggctgtag cgcgttcaat caactggttg agcacatgca aaccgctacg ctggaatacc
    36421 agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa gtcagttttg
    36481 tttacgataa tcaactactg catctcaaac agggcatttc agcgtctggt gcacgttaca
    36541 ctgacggaat ctatgttttc tggtcgaaag gcgatgaagc gactgtctat aaacgcgacc
    36601 gcatcgtatt gaataactgt cagttacaaa atccacagcg ttgagatttt tccaggggcg
    36661 gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga taacgacgaa
    36721 ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg ccgtcgcgat
    36781 cttcccgccg atccattaac cctttttgaa cgttggctct ctcaggcgtg tgaagccaaa
    36841 ctggcagacc ctaccgcgat ggtggtcgct accgtggatg aacatgctca gccttatcaa
    36901 cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac caacctcggt
    36961 agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt cccgtggcat
    37021 acccttgaac gccaggtgat ggtgatcggt aaagcagaac gactttcgac tctcgaagtg
    37081 atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt ttcgaagcag
    37141 tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct gaagcagaag
    37201 tttcaacagg gcgaagtgcc attgcccagc ttttggggcg gttttcgcgt cagccttgaa
    37261 cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt gtaccagcga
    37321 gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa aatcttgctt
    37381 taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca tctggcgaaa
    37441 agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg gcaagcagta
    37501 acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac gaggaagcgt
    37561 tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat cctaccgctg
    37621 acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc cagcaggcgg
    37681 gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac ccgagcttca
    37741 aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg gacaaaatcc
    37801 gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct atcgcggcga
    37861 acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat attggcaaac
    37921 acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc aaccgtgaag
    37981 atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat gacttcgcct
    38041 gtctgaacaa acagtacggt gtggtgctgc aaattggtgg ttctgaccag tggggtaaca
    38101 tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt ggcctgaccg
    38161 ttccgctgat cactaaagca gatggcacca aatttggtaa aactgaaggc ggcgcagtct
    38221 ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc aacactgcgg
    38281 atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa gagatcaacg
    38341 ccctggaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat gtactggcgg
    38401 agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa cgtattaccg
    38461 aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa cagctggcgc
    38521 aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag gcgctggtcg
    38581 attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc aatgccatca
    38641 ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa gatcgtctgt
    38701 ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt tgctggaaat
    38761 aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta tacagggaac
    38821 atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc gggtaacagt
    38881 gcggcagagt ttcctatgcg ccgcctgggc gcgaacgtct ggccgctgaa caccgttcaa
    38941 ttttctaatc acacccaata cggcaaatgg actggctgcg tgatgccgcc cagccattta
    39001 accgaaattg tgcaaggcat tgccgccatt gataaattac acacctgtga tgccgtgtta
    39061 agtggctatc tgggatcggc ggagcagggt gaacatatcc tcggtatcgt ccgtcaggtg
    39121 aaagccgcga atccgcaggc gaaatatttt tgcgatccgg taatgggtca tccggaaaaa
    39181 ggctgtatcg ttgcaccggg tgtcgcagag tttcatgtgc ggcacggttt gcctgccagc
    39241 gatatcattg cgccgaatct ggttgagctg gaaatactct gtgagcatgc ggtaaataac
    39301 gtcgaagaag cggttctggc agcgcgcgaa ctcattgcgc aagggccaca aattgtgttg
    39361 gttaaacacc tggcgcgagc tggctacagc cgtgaccgtt ttgaaatgct gctggtcacc
    39421 gccgatgaag cctggcatat cagccgtccg ctggtggatt ttggtatgcg ccagccggta
    39481 ggtgttggtg atgtgacgag cggtttactg ctggtgaaac tgcttcaggg ggcaacgctg
    39541 caggaggcgc tggaacatgt gaccgctgca gtctacgaaa tcatggtgac caccaaagca
    39601 atgcaggaat atgagctgca agtggtggct gctcaggatc gtattgccaa accagaacat
    39661 tacttcagcg caacaaagct ctgaaatatc tcatttcggc ccacaacgac ctgtgggccg
    39721 atggatatta ctttaagcct tccgctgaca gcgcgtcttg tacttccgga cgttcagcca
    39781 tacgttgcat aaatgctgca atgtgctcta acccttccag attcagtttc accgcgtatg
    39841 cccagcgcag aaccgtaaac agataggcat cagcaattgt aaatctttgc ccgcagatcc
    39901 agtgctcatc cttcagtgcc tcgttcacat attgcagctt cttctccagc tgcgcgcgaa
    39961 ctgtcggttt gtactcttcc ggtgtatcag ggcgaaacag aggtgtgaaa cctttatgca
    40021 gctcggtggc gatgtaattc agccattcga tggttttata gcgggaaata ctgtttaccg
    40081 gtgccagcaa ctggcggtcg gggacgctgt cggcaagata ctgcataatc gctacgcctt
    40141 ccgtcagcaa agtaccgtca tccagcagca atgcaggcac ctgcccctta gggttaacgg
    40201 caaagtaatc gtcaccgttt tcgagacgtt ttttcattaa atccacactg acgagggtaa
    40261 aatcctttcc gctctcacgc agggtgatat gggaagcgag agagcaggca cccggtttgt
    40321 agaacaattt catcggtaac tccttttgtc tgtggtttaa agtatggtag tgcgttgctg
    40381 aacaaaaaaa aagccgctaa cgaaaagtta gcggcttgta aaagagtttc cctgaatttt
    40441 acgctacagc agctttcgcc gctttgtctg cagcgtcatc ctgcgtcata cggtgcagtt
    40501 taggcgcggt cagcagcatc agtactgcaa taatggcagt agcgacacca atctgcaaga
    40561 atacgcgacc ataaacttcc agtgacatca gcgggtcggt aacgttatcc ggcacagcca
    40621 tcatacccgc aacataacca ccaatcaggt ttgcaccggc agtggtcagg aaccagctac
    40681 ccataatgaa gcccatcaga cgctgcggaa cgagttgtgc aaccattgcc agacccagac
    40741 cagagatcat cagttccccg atgctctgca ggccatagct tgcgaccagc cagcttacag
    40801 acacgatacc agcgtcagac gcgaatttcg cacccagcgg cagaatcagg aacgcaccag
    40861 aacacatcac catgccgatt gcaaacttgg ttggcatcgg cagggtatcg cccatcttgt
    40921 tatagatagc ggccagaatc ggactaccga tgatgatcca gaacgggttc agtgcctgat
    40981 actgctcagg ttctacggcc agacccagaa tggagtgctc aacgttacga atcgcaaaga
    41041 agttcagtga tgtcggcatc tggctgtaca gcacgaagaa gatgattgct tcgagcatca
    41101 ggatgaaggc aacgatcatt ttacgacgcg cagcaccttt catcgcgaag gcttctttac
    41161 cgaagataac cacgataccg aaggcaacaa cgcccagcgc catacgcgca acttcctgat
    41221 tgtgcagcag ccaggtggcg atagcgatca gtgccacaac accaataatg gttagcagca
    41281 ggttacggta gttgataggc tcgaagtctg gttttgaacc gtactgttta acccagcgtt
    41341 ggcagaaggc gaagttaacg atagtgatca gcaggcctac aacgctcaat gcaaacgcaa
    41401 cgctccagcc gtatttcgcg gccagccacg gcgtagcaat catagagaag aaagagccga
    41461 tgttgacgga catgtagtac atggtgaatg caccgtccag acgcgggtcg tttttctcat
    41521 agcatgtaga aagcagagaa gacgggttag ccttaaacag gccgttaccg accgcaatag
    41581 ccgccatacc catataaaca ataccggcgt cgtgaccaga ccaggcaacc agagcataac
    41641 caatcgccag cacaatagcg ccgagcataa ttacgcgttt agtacccagt accttgtcac
    41701 ctaaccagcc gccgatagcg accagaccat aaaccagggc actaaaggaa gagaaaaggg
    41761 tgattgaatc cgcttcagac atacccagtt gtttaaccag gtaaacagcc ataattcctt
    41821 gtaggccgta ataaccaaaa cgttcccata actcaatcga gaagatgaga tagaacgcct
    41881 tcggttgttt gaaagcgttc aaactgacgc tttcagttgg tttttggttt gcagtggaca
    41941 cttttacctc tttttttaca tcccatatta acgggggtgc tttctcgcgt tatgtctaaa
    42001 aaacatacgc gcaattgtta tagtgggagg ggaaacggca ggtaatgttc actatcttgc
    42061 actttcagac aagtcctttg taataatctg ttacatataa ctgaggcggg gaattcaacc
    42121 attccgggaa atgttatcca gcgttaaatt tttcaaaaaa cgtaacggca gattttttca
    42181 ctgcactgag aggggggtga tggcttaacg gcgatatgtt ctgctattta gcgtttttac
    42241 tagtgagata gtccagtttc tgaaaaatag ccagtgtaat gttttgtagg tcaatatttg
    42301 ttgcgtgtgt aaatttaaat gattttaacc tgatttttgc aatgttttta cattgccaca
    42361 gcatcgtgat cttgatcacg aatgaataga aaatttcgct attaaaaaaa caattaacct
    42421 gcatttttgg ttatccgcaa acggattacc tgatgataac gctaagcaat ggcatcattc
    42481 acgttaaaaa gatgttttaa aagaggataa agaaaggtta tcaatggggt aatcggtgtt
    42541 accccttttc ttcagatgtc aactttctct ttgtattcac aaagatcttc aataatacaa
    42601 gagccacagc ggggcttgcg ggcaatgcag gtataacgcc cgtgcaggat caaccaatgg
    42661 tggcagtcga ctttaaactc tgccggaacc acttttagta gcttttcttc tacctgttcg
    42721 acatttttcc ccggcgcaaa ttgagtacga ttacaaacgc ggaaaatgtg cgtgtcgaca
    42781 gcaatagtcg gccagccgaa tgcagtgttt aatacgacgt tggctgtttt acgacctacg
    42841 ccgggcaggg cttcaagcgc agcacgatct tccggaacca agccattatg ctgctccagt
    42901 aagatacggc aggttttgat gatattttct gctttgctgt tataaagccc aatcgttttg
    42961 atataggttt taaccccttc aacgcccagt tcaagcatcg ctgcaggcgt attcgccacc
    43021 gggtagagtt tcgccgtcgc cttattaaca ctgacatcgg tcgcctgagc ggaaagcagt
    43081 acggcaatca gcaattcaaa aggcgaactg aaattaagct cggtggtggg atgaggattg
    43141 ttctcacgca ggcgagtgag gatctctagg cgttttgctt tgttcatcag acattccctg
    43201 tttcaccgtt tggcaatgca cgttcggcag ctgcttcagt acggcgcttt ttcattcttt
    43261 catcaatcag gtattttcct gccagcatca gtcccaggcc aataaatgca cctggaggca
    43321 gcatcgccag caggaaaggg gagtcggtgt ggaaaatttc cacgcgtaat acttttgccc
    43381 agctacctaa cagcgcatct gcaccgtcaa acaatgtgcc attgccgata atttcgcgta
    43441 gtgaacccag cacgaacatg gcgcaggttg cgcccatacc aattgaaaag ccgtccagtg
    43501 ccgaaagcgc cggacctttt ttggcggcga aggcttcagc gcggcccaca acgatacagt
    43561 tagtgacaat cagcggaata aaaatcccta atgattgata caggccaaag gcgtaggcgt
    43621 tgatcagcat ctgtacagcg ctgaccaccg aggcgatgat catcacgtaa atgggaatgc
    43681 ggatctcggc tggcgtccag tgacgcagcg tcgaaatggt caggttggtc agcgtcagta
    43741 ccagcgtagt cgcaagtcct aaacccagag cgttagtggc agtggacgtg accgccaaca
    43801 gaggacaaag gccgagcaac tggaccagcg cagagttgtt tttccacaac ccctgaacaa
    43861 taacgtcttt aatttcgctc acgctttatt ctccacaggc aggaagttga gaaagttgtg
    43921 ccggtaacgt ctgagcgtac aatccggcgc gttttaccgc attaaccacc gcgcggggag
    43981 taatcgtcgc gccggtgaac tggtcgaaat caccaccatc tttcttcacc gcccagtgcg
    44041 catcatctgc accactgatt tttttacccg caaaatgggt gatccagtca gaaaggcgca
    44101 gttcgatttt atcgccaagc cctggcgttt cgtggtgctc tgtcacgcgc gtgccaagta
    44161 ccgtgccgtt aaaatcggct cccaccagca gctgaatcgc accggaatag ccatctggcg
    44221 cggttgcttc cagaacggcg gctaccggtt tgtcatcctg tttggcgatg taaacccgat
    44281 gctcaccttt acctaactct ggcgcagtta ccagatagca actctgtgcc agcgcattgt
    44341 tatagcgttc ggctggcagc acctgatcaa ataatgcctt ttgttgcaga ctggcctgtt
    44401 cagcaatcgt cgttttggtc atctggttga tggccgcagt taaccctgtt gaacccgctg
    44461 caaatagcgc taacgtaatg ccgtgttttc ggatagtttt cagcatggtt taccctttgc
    44521 gatggccgta gacgcgcgga cgcgtgtagt aatcgatcag aggaaccgtg atgttcgcca
    44581 gcaggacggc aaaagccacg ccgtcaggat agccgccgaa actgcggatc aaccagacta
    44641 ataagcccgc cagcgcgccg aaaataagac gaccacgatt ggtcgtagaa gcggtaaccg
    44701 ggtcagtcaa aataaagaat gcgccgagca tggtcgcacc agacagcaga tgaatttgcg
    44761 gtgctgccag tgtttctggt gagaacaacc agcccaacgt tgcgcataac gccagcgtta
    44821 ctaagaagct gaggggaata tgccagcgaa tcgctttctg ccatagcaac cacaggccgc
    44881 cagccagcca ggcgagattt acccattgcc aaccagcgcc cgccagaata ccgctgtaga
    44941 tcggatattg cataatctgt tcaaccgaat gaccggcacg gacagaggtt ttaaatgtat
    45001 ccagcggtgt cgcctgacta atgccatcaa tacctaagcg tagtgtgttc atatcaccac
    45061 cactggcggt atgtccgctg aaaataacct gtatggcgtc gataaaacca gggatgttga
    45121 ccgcaatttc atgtggcggt aaccagctgg tcatctgcac cgggaaggag atcagtaaga
    45181 ccacataacc aatcattgcc ggattaaacg ggttttgccc cagaccgcca tacaactgtt
    45241 tagcgatgat caccgcaaac accgtaccca gcacgaccat ccaccatggc gcgagggggg
    45301 gaatacttac cgccagcaat aagcctgtca gcaatgctga gttatctttc aacgttgcgg
    45361 ctaccgactg cttgcgtagt ttgagtacga gagcttcggc taacagagca ctaaccgatg
    45421 ccaacaggat ctgaacgaga gtaccccaac caaaaaacca cagttgcgct gcgattcctg
    45481 gcacggctgc gagcaacacc aacagcataa tgcgcgatgt ctggcgctgg ttatgggtat
    45541 aaggggagct agctattctg aataccattt agtcctcgtt tacaaccttc tgctgggcgg
    45601 cttttttggc ctgagcgcgg gcaatagccg cggcaactgc cgctttgcgc ggatcaacct
    45661 gttcttctgg ttctgcgtta gtctgttgct gttccagctt gcgcgctttg gcac
//
LOCUS       DAZFKM010000024        43747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFKM010000024 DAZFKM010000000
VERSION     DAZFKM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..329
                     /locus_tag="ACHKAY_001460"
     CDS             81..329
                     /locus_tag="ACHKAY_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5700163.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHKAY_001461"
     CDS             307..1437
                     /locus_tag="ACHKAY_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5700164.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKAY_001462"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHKAY_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO5700165.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHKAY_001463"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHKAY_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKAY_001464"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHKAY_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO5700166.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKAY_001465"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHKAY_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO5700167.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKAY_001466"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHKAY_001466"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO5700168.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKAY_001467"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHKAY_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO5700169.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKAY_001468"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHKAY_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO5700170.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..7124
                     /gene="kch"
                     /locus_tag="ACHKAY_001469"
     CDS             5871..7124
                     /gene="kch"
                     /locus_tag="ACHKAY_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO5700171.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHKAY_001470"
     CDS             complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHKAY_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO5700172.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7495..8955
                     /gene="cls"
                     /locus_tag="ACHKAY_001471"
     CDS             7495..8955
                     /gene="cls"
                     /locus_tag="ACHKAY_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO5700173.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8990..9319
                     /gene="yciU"
                     /locus_tag="ACHKAY_001472"
     CDS             8990..9319
                     /gene="yciU"
                     /locus_tag="ACHKAY_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO5700174.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHKAY_001473"
     CDS             complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHKAY_001473"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO5700175.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHKAY_001474"
     CDS             complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHKAY_001474"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO5700176.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHKAY_001475"
     CDS             complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHKAY_001475"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO5700177.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHKAY_001476"
     CDS             complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHKAY_001476"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO5700178.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHKAY_001477"
     CDS             complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHKAY_001477"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO5700179.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15242..15538
                     /locus_tag="ACHKAY_001478"
     CDS             15242..15538
                     /locus_tag="ACHKAY_001478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700180.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(15696..16343)
                     /gene="ychE"
                     /locus_tag="ACHKAY_001479"
     CDS             complement(15696..16343)
                     /gene="ychE"
                     /locus_tag="ACHKAY_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO5700181.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16374..16427)
                     /gene="ychE"
                     /locus_tag="ACHKAY_001480"
     CDS             complement(16374..16427)
                     /gene="ychE"
                     /locus_tag="ACHKAY_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO5700182.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16820..19495
                     /gene="adhE"
                     /locus_tag="ACHKAY_001481"
                     /gene_synonym="adhC"
     CDS             16820..19495
                     /gene="adhE"
                     /locus_tag="ACHKAY_001481"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO5700183.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            19619..20515
                     /locus_tag="ACHKAY_001482"
                     /pseudo
     CDS             19619..20515
                     /locus_tag="ACHKAY_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(20797..21414)
                     /gene="tdk"
                     /locus_tag="ACHKAY_001483"
     CDS             complement(20797..21414)
                     /gene="tdk"
                     /locus_tag="ACHKAY_001483"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO5700184.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            22019..22432
                     /gene="hns"
                     /locus_tag="ACHKAY_001484"
     CDS             22019..22432
                     /gene="hns"
                     /locus_tag="ACHKAY_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO5700185.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(22576..23484)
                     /gene="galU"
                     /locus_tag="ACHKAY_001485"
     CDS             complement(22576..23484)
                     /gene="galU"
                     /locus_tag="ACHKAY_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO5700186.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(23686..24699)
                     /gene="rssB"
                     /locus_tag="ACHKAY_001486"
     CDS             complement(23686..24699)
                     /gene="rssB"
                     /locus_tag="ACHKAY_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO5700187.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(24791..25696)
                     /gene="rssA"
                     /locus_tag="ACHKAY_001487"
     CDS             complement(24791..25696)
                     /gene="rssA"
                     /locus_tag="ACHKAY_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO5700188.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            25809..26267
                     /gene="ychJ"
                     /locus_tag="ACHKAY_001488"
     CDS             25809..26267
                     /gene="ychJ"
                     /locus_tag="ACHKAY_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO5700189.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            26317..27159
                     /gene="purU"
                     /locus_tag="ACHKAY_001489"
     CDS             26317..27159
                     /gene="purU"
                     /locus_tag="ACHKAY_001489"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO5700190.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            27319..27403
                     /locus_tag="ACHKAY_001490"
     tRNA            27319..27403
                     /locus_tag="ACHKAY_001490"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27353..27355,aa:Tyr,seq:gta)
     gene            27451..27582
                     /locus_tag="ACHKAY_001491"
     ncRNA           27451..27582
                     /ncRNA_class="other"
                     /locus_tag="ACHKAY_001491"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(27766..28443)
                     /gene="narI"
                     /locus_tag="ACHKAY_001492"
     CDS             complement(27766..28443)
                     /gene="narI"
                     /locus_tag="ACHKAY_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5700191.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(28443..29153)
                     /gene="narJ"
                     /locus_tag="ACHKAY_001493"
     CDS             complement(28443..29153)
                     /gene="narJ"
                     /locus_tag="ACHKAY_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5700192.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(29150..30688)
                     /gene="narH"
                     /locus_tag="ACHKAY_001494"
     CDS             complement(29150..30688)
                     /gene="narH"
                     /locus_tag="ACHKAY_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5700193.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(30685..34428)
                     /gene="narG"
                     /locus_tag="ACHKAY_001495"
     CDS             complement(30685..34428)
                     /gene="narG"
                     /locus_tag="ACHKAY_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO5700194.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKISNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(34943..36334)
                     /gene="narK"
                     /locus_tag="ACHKAY_001496"
     CDS             complement(34943..36334)
                     /gene="narK"
                     /locus_tag="ACHKAY_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO5700195.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            36673..38469
                     /gene="narX"
                     /locus_tag="ACHKAY_001497"
     CDS             36673..38469
                     /gene="narX"
                     /locus_tag="ACHKAY_001497"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO5700196.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            38462..39112
                     /gene="narL"
                     /locus_tag="ACHKAY_001498"
     CDS             38462..39112
                     /gene="narL"
                     /locus_tag="ACHKAY_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO5700197.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(39113..40507)
                     /gene="ychO"
                     /locus_tag="ACHKAY_001499"
                     /gene_synonym="iatA"
     CDS             complement(39113..40507)
                     /gene="ychO"
                     /locus_tag="ACHKAY_001499"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO5700198.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            40692..41045
                     /gene="ychN"
                     /locus_tag="ACHKAY_001500"
     CDS             40692..41045
                     /gene="ychN"
                     /locus_tag="ACHKAY_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO5700199.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(41089..41784)
                     /gene="chaC"
                     /locus_tag="ACHKAY_001501"
     CDS             complement(41089..41784)
                     /gene="chaC"
                     /locus_tag="ACHKAY_001501"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO5700200.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(41942..42172)
                     /gene="chaB"
                     /locus_tag="ACHKAY_001502"
     CDS             complement(41942..42172)
                     /gene="chaB"
                     /locus_tag="ACHKAY_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO5700201.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            42442..43542
                     /gene="chaA"
                     /locus_tag="ACHKAY_001503"
     CDS             42442..43542
                     /gene="chaA"
                     /locus_tag="ACHKAY_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO5700202.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT        11146 a        11102 c        10447 g        11052 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
     6001 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
     6061 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
     6121 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
     6181 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
     6241 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
     6301 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
     6361 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
     6421 caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt
     6481 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
     6541 ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag
     6601 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     6661 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     6721 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     6781 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     6841 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     6901 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     6961 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     7021 atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg
     7081 aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag
     7141 agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac
     7201 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     7261 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     7321 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     7381 gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     7441 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     7501 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     7561 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     7621 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     7681 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     7741 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     7801 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa
     7861 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     7921 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     7981 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     8041 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     8101 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     8161 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     8221 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     8281 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     8341 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     8401 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     8461 caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc
     8521 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     8581 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     8641 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     8701 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     8761 gatatgcgga gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt
     8821 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     8881 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     8941 agtccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     9001 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     9061 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     9121 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     9181 tttgaaccca gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     9241 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     9301 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     9361 ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     9421 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     9481 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     9541 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     9601 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga
     9661 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa
     9721 attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga
     9781 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     9841 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     9901 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     9961 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
    10021 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
    10081 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
    10141 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
    10201 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
    10261 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
    10321 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
    10381 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
    10441 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
    10501 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
    10561 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
    10621 catcgcgcgc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
    10681 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc
    10741 gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag
    10801 tgggttcatc cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc
    10861 gctgacgcat gccgccagaa aattcgtgcg ggtacatttt catgcgttta cgcgcttccg
    10921 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
    10981 tcttatgcag catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg
    11041 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat
    11101 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt
    11161 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
    11221 ctacaatacc cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga
    11281 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
    11341 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
    11401 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
    11461 cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt
    11521 cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag
    11581 gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac
    11641 caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc
    11701 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
    11761 aatacgagcc atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt
    11821 ttgaccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
    11881 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
    11941 ataaagtgtc ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat
    12001 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
    12061 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
    12121 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
    12181 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
    12241 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
    12301 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
    12361 tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact
    12421 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca
    12481 taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa
    12541 gagagcacgg gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt
    12601 aaccctttcg cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca
    12661 cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc
    12721 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
    12781 aatatcatga ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc
    12841 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
    12901 ctaaccccca gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga
    12961 aaactggatg caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa
    13021 tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga
    13081 tgatatttct cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa
    13141 gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc
    13201 ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc ttctggcctg
    13261 tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta
    13321 ttgccattag tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt
    13381 gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg
    13441 aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg
    13501 cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata
    13561 atgcgcggta ttcatcgagc tgttcgaaag catggtgttc aggaaggaag ttggttcgtt
    13621 gtagtcagca caccagcctg cacgggccac atcaaaagta ccctggtgac gggtgtcgag
    13681 gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt tcttccacaa
    13741 tgaagaggca gcaatcgcca gctttttatg cagatcggag gtgttataca acaggttgat
    13801 ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt
    13861 acgtttttcc tggctccagc caaaccattc cggctgagtc aattttgcgc catcagtata
    13921 cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat
    13981 atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt
    14041 ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg
    14101 gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttataag tcatgtcgat
    14161 ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac
    14221 ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg
    14281 ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg
    14341 ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag ttgatgggtg
    14401 aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc
    14461 atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat
    14521 accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac
    14581 agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt
    14641 cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc
    14701 gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg
    14761 gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga
    14821 accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc
    14881 tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct
    14941 cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag
    15001 catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct gtcaggttac
    15061 tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct
    15121 tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt
    15181 aattgttaat tattctcatt accaataaat aatcgtctgg cggttgcccg gaaagtacca
    15241 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga
    15301 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa
    15361 tagagattgg ctaagcatgt ctatcttttc tgattacagt tctagtagtg aaatgcacaa
    15421 taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg gaattactaa
    15481 tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa taacatgatg
    15541 gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa gaatattttt
    15601 acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat gacgaagtaa
    15661 acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc ggggaaaatc
    15721 cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag ccccataata
    15781 cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg
    15841 aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc aaacagatag
    15901 ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc tggccccgcc
    15961 atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt ttctgatttt
    16021 tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc
    16081 accaccagga tgcccccggc gatacggaac gaatcaattg atataccaaa aagttgcaga
    16141 atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag gttggctgta
    16201 aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat aaagacggga
    16261 ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa gtaaacggga
    16321 aaatcaaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata atgttagcca
    16381 taaataaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt tcacctccta
    16441 actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta
    16501 gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa gcgatgctga
    16561 aaggtgtcag ctttgcaaaa atttgatttg gatcacgtaa tcggtaccca gaagtgagta
    16621 atcttgctta cgccacctgg aagtgacgca ttagagataa taactctaat gtttaaactc
    16681 ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat aggttaagca
    16741 aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta aaaaagttta
    16801 acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg cactcgtaga
    16861 gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag tagacaaaat
    16921 cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt
    16981 tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga
    17041 atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg aagacgacac
    17101 ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg ttccgaccac
    17161 taacccgact tcaactgcta tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat
    17221 tatcttctcc ccgcacccgc gtgcaaaaga tgccaccaac aaagcggctg atatcgttct
    17281 gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg atcaaccttc
    17341 tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc tcgcgactgg
    17401 tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc
    17461 gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt
    17521 actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt ctgttgttgt
    17581 tgttgactct gtttatgacg ctgtacgtga acgttttgca acccacggcg gctatctgtt
    17641 gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc
    17701 ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct ctgtaccaga
    17761 aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca
    17821 tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga
    17881 aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc tgtacactga
    17941 ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat
    18001 cctgattaac accccagcgt ctcagggtgg tatcggtgac ctgtataact tcaaactcgc
    18061 accttccctg actctgggtt gtggttcttg gggtggtaac tccatctctg aaaacgttgg
    18121 tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca
    18181 caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc tggatgaagt
    18241 gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg
    18301 ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg aagtcttctt
    18361 cgaagtagaa gcggacccga ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc
    18421 cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat
    18481 catgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc tgcgctttat
    18541 ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc
    18601 tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg taactgacga
    18661 cgctactggt cagaaatatc cgctggcaga ctatgcgctg actccggata tggcgattgt
    18721 cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg gtctggacgc
    18781 agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct ctgatggtca
    18841 ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc acgaagggtc
    18901 taaaaatccg gtagcgcgtg aacgtgttca cagtgcagcg actatcgcgg gtatcgcgtt
    18961 tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt cccagttcca
    19021 tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct acaatgcgaa
    19081 cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg
    19141 ttatgctgaa attgccgacc acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa
    19201 gatcgagaaa ctgctggcat ggctggaaac gctgaaggct gaactgggta ttccgaaatc
    19261 tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata aactgtctga
    19321 agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga tctccgagct
    19381 gaaacagatt ctgctggata cctactacgg tcgtgattat gtagaaggtg aaactgcagc
    19441 gaagaaagaa gctgctccgg ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt
    19501 agcgctgtct ggcaacataa acggcccctt ctgggcaatg ccgatcagtt aaggattagt
    19561 tgaccgatcc ttaaactgag gcactataac ggcttccaca acagggagcc gttttcttat
    19621 gccacttctt aatgatctgc tcgatttcag tgaccatccg cttatgcctc cgccctctgc
    19681 acaactattt gcagaacacc ttcccaccga gtggatacaa cactgcctga cgctttctgc
    19741 tcatgcgacc gttcgccgcc gtcgtttacc gggggacatg gttatctgga tggtggtgca
    19801 atgagccaat taccgatgtt gttcgccgtc tgaacctgag cgcggatggc gaagcgggga
    19861 tgaacctgct ggcccgcagc gctgtcaccc aggcgcgtca gcgcgtgggg gccgccccag
    19921 tggaatggct cttccgccag accgcacaga caggggcgcg gaacgttacc tgaaggatga
    19981 ctggcacggc ctgcaacttt ttgccattga tggcgcacag ttcaggacac ctgataaacc
    20041 cgagctgcgt gaatattatg ggtctgccaa cacatccact aagcggcaga acgcctaccc
    20101 ggttatgcgt ctggtggcct taatgaacct gggaagtcac attctgctga atgccgtgac
    20161 cgcaccttac cggcagagcg aaactgtgct ggcccactcc atgctcgcca ccatcccgga
    20221 taactctatt acgttgtttg ataaactttt ttacagcgaa gacctgctgc tgacgctgaa
    20281 ccagaagggt tgtaaccgcc actggctact gcctgcgtgg aagaatatcg catcagaaat
    20341 gatagagctg ggaaacacgg cgtcaacagg gaccatcccg aaacgactgg agcatcttcg
    20401 gggtgctctg gaagtggtgt tcataacaaa acgcccccga ccatcaaggc cgaggtcggt
    20461 taagatatca aaaacccgtt atcctgtgaa acatagtgcc gctcctctta agtgaacggc
    20521 attgcccttc tggggccgta ttttttgttt acccaaagca acttttccat aaaccgacag
    20581 cattagcctt catcatattt gcgacgatgt ataacgccta aacacaggga tattgtactt
    20641 tacaggtcac aagtcaacgt cggtgcttaa gagccctgtg aggcgtatag cggcgttaaa
    20701 aaactgccga gaagggtata tagcccggaa gaagtgcgta aaacgaactg acaggataaa
    20761 agtgccctgc tcaccctgtc agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg
    20821 aatagccgtt aatgagccga cttgtaacgc ctctttatag tgtttacggc atacagaaac
    20881 gtatcgttca ttaccaccaa ttaccacctg ctcaccttcg ttataaggtc tgcctgcttg
    20941 atcaagacgc agcaccatgc ttgctttacg gccacaaaaa cagatggttt ttaattcaac
    21001 cagtttgtcg gaccatgcca gtaagtattg gctgccaata aataattcac ctcgaaaatc
    21061 ggtacgtaaa ccataacaaa gtacgggtat atcgagttga tcgacaacct ccgataattc
    21121 atatacttgt tgtctggtta aaaactggca ttcatcaacc agtacgcaat gaattgcctg
    21181 ctgttcatgt tccgcacgaa tctcatcaaa taatgatgaa ttttggttaa ataattttgc
    21241 aggcgatgac aaacctatac gcgaactgac tttcccggca ccaaagcgat catcaatttc
    21301 tgccgtatat acgacagtgc gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa
    21361 tgctgtagac ttacccgcat tcattgcgga atagtagaaa tatagctgtg ccatcagcca
    21421 caggccctca atgatatgaa ataaagttgg ctggagttta tcataattcg tcagtttttc
    21481 agctcaacag tatgcacaga agtgggtata tgcgttctcc cttacgaagc cttgcataat
    21541 ccttctgagc tatcattaca actgcctcgc ttgttataag cgggtaaatg actgctggta
    21601 actattcaca atctttaacc tgttgcgcaa gtaatagccc tctgttgacc tccaggagat
    21661 agtgcaatac taagtccatg ctcttattgc gactgttcta cttttcatca ttcgcttaat
    21721 agggaattct cgtaaacaca actaatacag aagactgaaa ggtcgtcagc ctacgataat
    21781 ctccccataa aatgtgacat gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg
    21841 gtgtaaatag ggctatatgc cgcgtctttt ctggctaatt ttatgaaaag atatttattg
    21901 gcggcacaaa ataaagaaca attttgaatt ccttacattc ctggctattg cacaactgaa
    21961 tttaaggctc tattattacc tcaacaaacc accccaatat aagtttgaga ttactacaat
    22021 gagcgaagca cttaaaattc tgaacaacat ccgtactctt cgtgcgcagg caagagaatg
    22081 tacacttgaa acgctggaag aaatgctgga aaaattagaa gttgttgtta acgaacgtcg
    22141 cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata
    22201 tcgcgaaatg ctgatcgctg acggtattga cccgaacgaa ctgctgaata gccttgcttc
    22261 cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg gcaaaatata gctacgttga
    22321 cgaaaacggc gaaactaaaa cctggactgg ccaaggccgt actccagctg taatcaaaaa
    22381 agcaatggat gagcaaggta aatccctcga cgatttcctg atcaagcaat aatcttttgt
    22441 agattgcact tgcttaaaat cccgccagcg gcgggatttt ttattgtccg gtttaagaca
    22501 atttaataag aataagctat aaaaaaacgg cgtcgattgc tcaacgccgt ttcgtggata
    22561 acaccgatac ggatgttact tcttaatgcc catctcttct tcaagccagg ctttaaattc
    22621 cgtgccaagg gtgttatgac gaataccgta ttcaacgaag gcctgcatgt aacctaattt
    22681 attaccgcag tcatggctct tccctttcat atgataggct tccaccgttt ctttttcaat
    22741 cagcatatca attgcgtcgg tgagctgaat ttcatcacca gctcccggag gggtttttgc
    22801 cagcaacggc caaatatccg cgctaagtac gtaacgaccc acaatagcga gattagacgg
    22861 cgcaacatcc gctttcggtt tttctaccac accaaccatc ggtacgcttt cacccggcgc
    22921 taattcaacg cctttgcaat ccacaacgcc atatgcggtc acatcagcaa ccggttcaac
    22981 catgatctgg ctatgacccg tttcatcaaa gcggcggatc atctctgcca ggttatcctg
    23041 tgacaaatcg gattcatatt catccagaat aacatcaggc aaaataacag ctaccggttc
    23101 atcacccact accgggtgag cacacaatac cgcgtgtccc aggcctttcg ccagaccctg
    23161 acgaacttgc ataatagtca cgtgcggtgg acaaatagac tgcacttcat caagcagttg
    23221 acgttttaca cgtttttcca gcattgcttc cagttcaaaa ctggtatcaa agtggttttc
    23281 aatagagttt ttagatgagt gtgtaaccag cacaatttca gtaatgccag ccgcaataca
    23341 ttcattcacg acgtattgaa ttaatggctt atcgacaagt ggcagcatct ctttcgggat
    23401 ggctttcgtc gccggcaaca tcctggttcc taatcccgca acggggataa cggctttttt
    23461 gactttcgta ttaatggcag ccatttaaat tctcctggac tgttcgtgtt ttgaacgtgt
    23521 tcatatttct gtatcgcatc ccagtatatc agcaccccga aggggctaag ttctgaaaag
    23581 gaggcgagtt agcataatta agacaattac ttatacgaat ggcgacgata gtaccaccag
    23641 ccgacattag caggtaatgc aaatttagcc cgcgttatcg tttgctcatt ctgcagacaa
    23701 catcaagcgc agtcgaccac cggttcccca tatttggcat tgccaggcat cgcatcgctg
    23761 gctcaattga ttcaaataag cgttacctaa agtgcctaac ggaacaccat tactgatttg
    23821 cacctggtgt tcgccggtat ttaacgtcgc attcagaccc gcagaaacca gaatgagatt
    23881 tttcagttcg cgatgataat agccaactaa tagcggaaac tgccccggca gattggcctg
    23941 acgaagtaaa tggtttacct gcttcaataa cgcgcccaac tctggcaacc gttgattttg
    24001 gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg cgtaataaca aggcagcaag
    24061 tacgccatta tgtccagctc gggtgacatc aaggcaataa aatgccagat cgttttccga
    24121 aagtgcggca atatcaagca ccaggccggg tttgtccgcg gcaaccaatt gacgataatt
    24181 aacccggcaa tgggaaatca cctgctgaac cggcggttgt agttcctgta ataatttcgc
    24241 cgctgcggca gggttatcaa ccattgcatc ccagtcgcga aaaagccttt cctcttcctc
    24301 aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa accatctcgc gcaagcgatt
    24361 caaatcttta actggtttca gcaaaacatc ttcaacgccc agacgtaacg ctttggcaat
    24421 atctgccata ttttcggtgg cagatatcac cagaaccggt gtctggtcac ctctgttgcg
    24481 tatatgttcc agcagtttaa gcccgttcat tcgtggcatc gcgatatcac atatcatcag
    24541 gtctggagtg aaacctccca gcaactcaag ggcatccacc ccatcagccg ccagtaccgt
    24601 tgtcgctccc aatgaggaaa accacgaatc cagaagcgag cgaaataccg gctcatcttc
    24661 aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg ttctctcccc tgactggctt
    24721 tactcaatag tggcatgcta ttgccacctg cgcctgtcag aattaactta agtgtagata
    24781 aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag ttcgtccatt ttcttttcca
    24841 ctgccagctg tccggccgca atggcagcgt gcgcgcgatg gaaatcaagc gtagatattt
    24901 gcgggcaaac aggttgaatc agaatatcgg gcggatcacc tgccatgcgg ttccttttaa
    24961 ggcggttctc cagcacctgg atagaagtgg tcataatctc tgttgccgtt ggcgctgtca
    25021 ccgcccgacg tgtcgttatg ctgcctaacc tttctttcag acgcgcatgc cacggcagag
    25081 aatcaccatt ctcgctattt tcttcactga cattaaagga gagcaaatct tgttgcatca
    25141 aatgagcatc gtgctgcagg tcaaccgcta tcacaatatc agcccccaat gcacgcgtga
    25201 gggaaatagg aattgggtta acgactgctc catcaaccag ccagtagccg ttatgtgcaa
    25261 caggtgccat gagtcctgga atactgcatg atgcgcgaat agcaagatgg agatcgcctt
    25321 cagtaaacca taattcacgt cccgtactta aattggtggc aacagccgca aagcgacggg
    25381 aacaattttc gatctctgtt tccggcatta tttcgcggta ttgattgaag acacgctcgc
    25441 cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag gcgtaaaaca tcccaataac
    25501 tgaaagaggt cacccaatct tccagcgcag ataatcgatc gcatgcatag gcagcgccca
    25561 ccagcgaacc aattgaacat cctgcaacga tatcaatttc aatacccact ttttttagcg
    25621 cattaataac gccaatatgc gaccaacctc tcgccgcgcc agatcccagc gccagcccta
    25681 tttttatctt tctcattatt cccgcaagat tcccctggaa tgcaatcgta gccacatcgc
    25741 tactgctcgg ttaacatagt gctactcagg cggttaatac gccgttattg tttccaggga
    25801 gattatttgt gtctcagctt tgtccctgtg gtagtgctgt cgagtatagc ctatgttgcc
    25861 acccttatgt gtctggtgaa aaggttgcac ctgatccaga acatctcatg cgttcgcgtt
    25921 actgcgcttt tgtgatgcaa gacgcagatt atttaataaa gacctggcat ccctcttgtg
    25981 gagcagcggc gttacgtgcc gaattgatgg ccggatttgc gcatactgaa tggttgggat
    26041 tgaccgtatt tgaacattgt tggcaggacg ccgacaatat cggatttgtc agttttgttg
    26101 ctcgatttac cgaaggtggt aaaacaggtg caataattga acgttcgcga tttttgaaag
    26161 aaaacggtca atggtactat attgacggta cacgtccgca gtttggtcgc aacgatccct
    26221 gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg ccaataatgg ttgacggtac
    26281 ggtttagcaa acactctcaa caaggttttt ccagcaatgc attcactcca acgtaaagtt
    26341 ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac gtattaccaa tatttgctac
    26401 aagcacgagt taaatatcgt acagaacaat gaatttgttg atcaccgtac cgggcgcttt
    26461 tttatgcgca cggaactgga agggattttt aatgattcca ccctgctggc ggatctcgat
    26521 agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg ccggtcgtcg ccggatagtg
    26581 attctggtca ctaaagaagc gcattgcctt ggcgatttgt tgatgaaagc caattacggc
    26641 ggcctggatg tcgaaatcgc ggcagttatt ggtaaccacg atactttacg ttctctggtt
    26701 gagcgttttg atattccgtt tgagctggta agccatgaag ggttaagccg caacgagcac
    26761 gatcaaaaga tggcggacgc cattgatgct tatcaatctg actatgtggt gctggcgaag
    26821 tatatgcggg tattaacgcc ggaatttgtg gcacgcttcc cgaataaaat catcaatatt
    26881 caccattcat tcctgcctgc gtttatcggc gcacgtcctt atcaccaggc ctatgaacgt
    26941 ggtgtgaaga ttattggcgc aaccgctcac tatgtgaatg acaatctgga cgaaggccca
    27001 atcatcatgc aggacgttat tcatgtcgat catacctaca cagctgaaga tatgatgcgc
    27061 gcaggtcgtg acgtcgagaa aaacgtctta agtcgtgcgc tatacaaagt actggcacag
    27121 cgcgtctttg tttacggtaa tcggacgatt attctttaat cgccagcaaa aataactggt
    27181 tacctttaat ccgttacgga tgaaaattac gcaaccagtt catttttctc aacgtaacac
    27241 tttacagcgg cgcgtcattt gatatgatgc gccccgcttc ccgataaggg agcaggccag
    27301 taaaaagcat taccccgtgg tggggttccc gagcggccaa agggagcaga ctgtaaatct
    27361 gccgtcatcg acttcgaagg ttcgaatcct tcccccacca ccatcacttt tcaaaagtcc
    27421 gtaaactctc aaacgaatcc gcaattaaat attctgccca agcggggaag gatgagaagc
    27481 ttcgaccaag gttcgacttg agcgccagcg agagagcgtt gccgcaggca acgacccgaa
    27541 gggcgaagcg cgcagcgttg agtaatcctt cccccaccac catcacttcc ttaaataatc
    27601 aaaacctgtt actccgagct aattttgccc gatggcacca cgtttaccgg gcctacaata
    27661 tcctcccctg taggtcggat aagcgaagcg catccgacaa ctcaccccgc accgtgccca
    27721 taaaaaaacc ccgccgaagc agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg
    27781 caccagctgg tacttacgtg tcagatactc caccggtacg ctccagatgt gtaccagacg
    27841 cgagaacggg aacagcagga ataacgtcat ccccagcacc aggtgcagac ggaagataaa
    27901 cgccacacca tcgaggtgtt gagaagcgcc accgtggaag gtcaccaccg actgcgccca
    27961 gccaaccagt ttcatcatct cgctaccgtc catatgctga gcggagaacg gaatggtcaa
    28021 caggcccagc gcgcactgga taacgagcag cgacaggatc aggatatccg ctccggtagt
    28081 ggttgcacgc acgcgtgggc tgaacagacg acgcttcagc aacagcacgc cgccaatcag
    28141 acacagcacg ccgctggcac caccagcaaa catcgccatt ttctgtttca cttcaatcgg
    28201 cagccaggct tcatacatcc agtgcggcgt cagcataccg aagaagtgac caacaaaaat
    28261 ccccagaata ccgatatgga acaggttcga cgccaggttc atccctttgc gatccagcat
    28321 ctggctggac gccgcgcgcc aggtgtactg cccgtagtca taacgcagcc agctaccaat
    28381 caggaagacc gccccggcta tgtacgggta gatatcaaag aagaacatat tcaggaattg
    28441 cattagtgct gtcctccggt ggtgatattc agatattgcg gcgcgacggc accggcaaag
    28501 cgacgctgat gagcagtgat tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc
    28561 tgctcttctt cccagacagc atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca
    28621 attttttccg ccactttgtc gctgtcgata gcggtattgg ccaatttcag cagcagatca
    28681 aacatcacgg cataacggct ttcacgctgt tgcagacgcg cgctcagcaa tgccagaatc
    28741 ggcgcgatat ctttcaaacc ttccacggct tcgctttgcg gcagctgcga caggtactcc
    28801 agatacagcg gcagatggtc cggtaattcg cggctgttta actgcaagcc gtgctgctcg
    28861 tactgcgcca gcaggtccac catcgcctgg ccgcggtcac gggattcacc gtgaacatgt
    28921 tcaaatagca acagtgacgt ggcgcggcca cggtcgaaca gttcgctgta ctgggcctgg
    28981 gcatcgagcg gatccatcgc cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatct
    29041 tctttcgaca gatttttcga cgcagcaatc gcctcaaaca tctcttgttg atgctgccat
    29101 aaggcagcat ccggatattc aaggagacgc gatacaatca cgagttcgat catggatgcg
    29161 gctccgtttt gctggtcaca tcgatggcat cgatacgacg gctgttgaac agattgaatt
    29221 tggtatctga accgtggcag ccatcaccaa aggtaaagcc gcagccattt ttctccggga
    29281 aggcttcccg tgccagttca cgatgactac tcggcaccac aaagcgatct tcgtagttag
    29341 caatcgccag ataacggtac atctcctgtg cctgggcttc ggtcagaccg acctcttcca
    29401 gcgcacgggt atcaacttta ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca
    29461 acatacgttt cagtgcgcgc agtaccggtt tggtatcacc ggcggtcagc agattcgcca
    29521 gatactgtac cggaatacgc aagctttcga cgtctggcag aatgccgttg ctacccagct
    29581 cacccgcgtc tgctgcagac tgaatcggag acagaggcgg cacgtaccag accatcggca
    29641 gtgtgcgata ttctggatgc agcggcagcg ccagtttcca ttccattgcc attttataaa
    29701 ccggcgactg ctgtgcggct tcaataacgc tcagcggaat accgtcttta atcgcctgct
    29761 cgatgacttt cggatcgttc ggatcgagga acacctccag ctgacgctgg taaagatctt
    29821 tctcgttctc ggtgctggct gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa
    29881 gataacggat acgaccgaca caggtttctg agcacacggt cggctgaccc gcttcaatac
    29941 gcggatagca gaagatgcac ttctcagact taccgctctt ccagttgaag tagatttttt
    30001 tgtacgggca tccggtgatg cacatacgcc agccacggca tttatcctgg tcgatcagga
    30061 cgatgccatc ttcttcacgc ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg
    30121 ggttcaggca gtgttcgcac aggcgcggca aatacatcat aaaggtgttt tcgaactggc
    30181 tatacatcgc cttctggatg ttgtcgaagt tcttgtcttt cgccagtttg tcaaactcac
    30241 cacccaggtc atcttcccag ttcggccctt tttcgatttt cgccatccgt tccccggtaa
    30301 tcagcgaacg cggacgggca atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt
    30361 tctgatagtc aaaatcgaac ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag
    30421 cgaagatttt acccagcaac atggcacggt tacccatgcg cggctgcagt ttgccgttga
    30481 ttttacggat ccagccgcct ttgtattttt cctggttttc ccagtcagtc gggaagccct
    30541 gacccggctt ggtttccacg ttgttgaacc acgcgtattc cacgccttca cggctggtcc
    30601 agacgttttt acaggtaact gaacaggtgt ggcagccgat gcacttatcg agattcagca
    30661 ccatgccgac ttgtgaacga attttcattt tacgctctcc tgtacctggt cattgccttc
    30721 gccatctaac cagtcaatgt tcttcatctt acgcactaca acaaactcat cgcggttaga
    30781 acctacggtg ccgtagtagt taaagccgta tgccagatgg gcatagccgc cgatcatatg
    30841 cgtcggtttc ggcgtgatac gggtgaccga gttatggata ccaccacgct gttgggtaat
    30901 ttccgaacca ggcaggttaa cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc
    30961 cggaacacgc tggctgacaa ccgcacgggc agtcagagca ccgttgctgt tgaagacttc
    31021 aatccagtcg ttatcggcga tacccagatc tttggcatcg gcttcactca accagaccac
    31081 cggaccaccg cgacctaaag tcagcatcag caggttgtcg ctgtaggtgg agtggatacc
    31141 ccacttctgg tgcggcgtca ggaagttgag cgctttttcc gggttgccat tggatttctg
    31201 gcccatcact tctttcaccg aacgggtgtc gatcggcgga cgataaacca gcaggctttc
    31261 accgaaatca cgcatccatt ggtgatcctg atacagctgc tgacggccag agagcgtacg
    31321 ccacgggatc agctcgtgaa cgttggtgta accggcgttg taagaaacgt gttcatcttc
    31381 aaggccagac caggtcgggc tggagatgat tttgcgcggc tgtgcctgaa tatcgcggaa
    31441 gcgaatcttc tcgtcttctt tattcagcgc cagatgcgta tggtcacgac cggtaaactc
    31501 gctcagggca gcccaggctt tcacggctac ctgaccgttg gtttccggtg ccagcgtcag
    31561 gatcatctct gccgcatcaa ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc
    31621 cgctttggtg tagttgagct tacgcagcag atccatctcg ctctgggtgt tccaggcaat
    31681 ccctttaccg ccattactga ttttctccat cagcgggccg atagaggtaa agcgttcgta
    31741 agtcgccgga taatcgcgct ctacgaccat aatgtgtggc gcggttttac cagggatcag
    31801 gtcgcactcg ccttttttcc agtctttcac atccagcggc tgcgccagtt cagcggcaga
    31861 gtcgtgctgg ataggcagcg tgacgatgtc ggtttcttta cccagatgac cgacgcacac
    31921 ttcggagaat ttcttcgcga tggctttgta aatttcccag tcgcttttcg cttcccaggc
    31981 cggatcgacc gccgcagaca gcgggtgaat aaacggatgc atatccgaag tattcatgtc
    32041 gtctttttcg taccaggtcg ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga
    32101 cagacggaag tccagcgtaa ccaccagatc cagcttgcct tccagaccat tgtcctgcca
    32161 gtccacttct tccggcttca cgccgccctg ttgccccaga tctttaccct ggataccgtg
    32221 ctccgtcccc agcaggtact tgagcataaa ctcatgacct ttaccggaag aaccgagcag
    32281 gttagaacgc cagatgaaca ggttgcgcgg gtggttttta ccgttttctg gttgttctgc
    32341 cgcaaaacgg atggaaccct ctttcaggga tttcaccgta tagtccaccg gattcatccc
    32401 ggctttttcc gcttcgccag cgatagtcag cgggttagtg cctaactgcg gtgcagacgg
    32461 cagccagccc atgcgttccg cacggacgtt aaagtcgatc aagtgtccgg tatagcggga
    32521 tttgtccgcc atcggtgaca gcaactcttc cgccgtgacg gtttcataac gccactggct
    32581 ggagtggtta tagaaataag aagtgctgtt catgtgacgc gccggacgct gccagtcaag
    32641 ggcaaacgcc agcggctgcc agccggtttg cggacgcagt ttttcctgac ctacatagtg
    32701 cgcccagccg cccccgctct gaccgacaca gccgcagaaa atcagcatgt tgatcagacc
    32761 acgatagttc atatcgaggt gataccagtg gttcagcccc gcaccgacga taatcatcga
    32821 acgaccgtgc gttttatcag cgttatcggc aaattcacgg gcgatgcgaa taatctggct
    32881 gcgagaaacg ccggtaatct gctcggccca ggccggggta taagctttca catcgtcata
    32941 gctggttgca cagttaacgt cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag
    33001 atcataaacg gtggtcacca gggcggtgct gccatcagcc agttgcaggc gtttcaccgg
    33061 cagtttgtgc agcagcacgt tttccagttc cactttgttg aagtgttccg tgccgtcgcc
    33121 accaaagtac gggaagccta cctcagcgat ctcatcctgg ctacccagca ggctcagttg
    33181 cagctcggtt tcttcgccag ttttgccgtc gcgctgctca agattccatt tgcccttctc
    33241 gccccagcgg aagccaatag aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc
    33301 gacagttttc cattccggat tgttttcctg gcccagcgca tcaaccagat cagcagcgcg
    33361 cagcatacga cctgcagcgt agtaaccgtc gcgttcttcc agcatcacca gcatcggcat
    33421 gtcggtgtag cgacgcacat agtcggtgaa atactggctt gggttgtcga ggtggaattc
    33481 acgcagcatt acgtggccca tcgccagcgc cattgccgca tcggtgccct gtttcggtgc
    33541 cagccacaga tcgcacagtt tggcgatttc agcgtagtct ggtgtgacgg caacagtttt
    33601 ggtccctttg taacgcactt cagtaaagaa gtgagcatcc ggggtacgcg tctgcggtac
    33661 gtttgacccc caggcgatga tgtagctgga gttgtaccag tcagcagatt ccggtacgtc
    33721 agtttgctcg ccccaggttt gcggagacgc aggaggcaag tcgcagtacc agtcgtagaa
    33781 gcttaagcaa gtaccgccaa tcagcgagag atagcgtgca cccgatgcgt aagaaaccat
    33841 cgacattgcc ggaattggcg agaaaccagc aacacggtcc gggccgtagt ttttgatggt
    33901 gtaaacgtta gatgcggcga tcagttcgtt cacctcctgc caggaagaac gaacaaatcc
    33961 accgcgtcca cgcgcctgct taaagctttt cgctttatcg gcgtcttcaa tgatagaagc
    34021 ccatgcctca accggatcgc tatgcagcgc cttcgcttca cgccacattt tcatcaggcg
    34081 tttgcgcatc atcgggtatt tcaggcggtt ggcactgtaa agataccagg agtagctggc
    34141 accgcgcggg cagccgcgag gttcatggtt tggcagatcc ggacgggtac gcggatagtc
    34201 agtctgctgg gtttcccagg tgaccagacc gtttttgacg tagattttcc agctgcagga
    34261 gccggtgcag tttaccccgt gggtagagcg gacgattttg tcatgctgcc aacgctggcg
    34321 atatccatcc tcccagtcac ggttggtatt gagaagctgg ccatgcccat cggcaaaggt
    34381 ttcacccttc tgtttgaagt agcgaaaccg gtccaggaat ttactcatcg gttttctcct
    34441 gtgggagcct gtcggccctc tgataaatcg acattgctaa tgtttaaggg caaggtaacg
    34501 ctctgaaaca gcgtgggaat tgataacgat caagtgtgat ggggatgaaa aataaagtaa
    34561 attcctaaga taccccttta ggataattaa cagttaaagc ataacatgct gtaaattaag
    34621 acgttttata cctgtagtta tttaaagcat gaagattttt atgcagatgg gtattaagga
    34681 gtattcccca tgggtaacat attaatggct cttaatgaaa tccggcgaat aagccatata
    34741 acattagtat gttgtaacta aataacgcat aaaaaaagcg cgacatcatg ccgcgcaaag
    34801 gataatcaaa tattttactc tatataattc ggtttacagg aaggcggcaa gtgaatgaat
    34861 cccaggagct tactaaagta agtgactggg gtgaatgaac gcagccgcag cacatgtaac
    34921 ccgaagtatg acgagtaacg gtttattttt tagaatgccg accatatacc gcccaggtaa
    34981 tcactacgca ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg
    35041 ttaatgccag cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg
    35101 cagagatgaa acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt
    35161 cagaaccccc ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca
    35221 tctggaaagt ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga
    35281 agaacgccat gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc
    35341 tgaaaatcgc catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag
    35401 ataatgcacc acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt
    35461 gcagaatctg aacatccggg aactgcgttt ttgacagcat tgcaaagccc gcggagaagc
    35521 cgatgaagga gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt
    35581 tgagtaccgg caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa
    35641 accacgccgc aatagtgaag atggcaagga acggcaccca tatccaggac gcattcgcca
    35701 gatacagctc agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg
    35761 acagtgatac caccagcgga gcaaccaact gcatgacgct gacgcccatg ttgcccagac
    35821 caccattcag acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg
    35881 ccatactgga ggcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga
    35941 cgctataagg cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca
    36001 gaataccggt gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt
    36061 atggaacacg taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat
    36121 cggtcgtaaa attaaagccg actttcggta ggttcaccgc aacagcgctg aacaacatcc
    36181 atacgcaaaa cgccagcagc agacagggaa cggaaatcca caggttgcgg ctggcaatac
    36241 gttgaccgcg ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc
    36301 cagtagccct ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg
    36361 tctgccacct tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac
    36421 tttatctatc attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta
    36481 tgagattttt cacggtgctg tagaatccct acccttaacg atgtaaagcg actaaccaca
    36541 cggcaaataa ggagtaactc tttccaggta tgggtatact tcagccaata gccgagaata
    36601 ctgccattcc agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag
    36661 gaagaggttt acatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg
    36721 cttattgtgt tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg
    36781 gtgcaaggcg ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa
    36841 agttaccgtc tgttggcagc agtgccatta agcgagaaag acaagccctt aattaaagag
    36901 atggaacaaa cggcatttag cgccgagttg actcgagcag cagaacgaga cggacaactg
    36961 gcgcaattac agggtttaca agattactgg cgtaatgaac tgatccctgc gttgatgcgt
    37021 gcacaaaacc gagaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcaa
    37081 ctggtatctg gttttgaccg caccacggaa atgcgcatcg agacagtggt actggtccat
    37141 cgggtaatgg cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg
    37201 cgactgctac aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat
    37261 tttacccaac gcgcaaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcgttg
    37321 aacaatatgt ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag
    37381 aaaaccgccg ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc
    37441 cgtttgcatt cccaagcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag
    37501 aatttaaccc tgctacgtga tatcgaactg cgggtttatg atactgatga tgaagagaat
    37561 catcaggagt ttacctgcca accagatatg acttgtgatg ataaaggctg ccagctctgc
    37621 ccgcgcggcg tattacccgt tggtgatcgc ggcacaaccc tgaagtggcg gctggctgac
    37681 tctcatacgc agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat
    37741 gatcaacaac aactggtgga taccctggtt gaacaactca ccgccacgct ggcgctggat
    37801 cgccatcagg aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc
    37861 gaactgcatg attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta
    37921 cagatgcagg gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac
    37981 gaactgaatg catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc
    38041 accgagcctg gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt
    38101 ggcttcccgg tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca
    38161 atccacttgt tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ctcgcaagcg
    38221 agtgaagtcg tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat
    38281 aacggctgcg gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg
    38341 cgcgaccgtg cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc
    38401 accgaagtgg tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc
    38461 catgagtaat caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac
    38521 tggcgtaaaa cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa
    38581 tggcgaacag ggtattgaac tggcggagtc tcttgatccc gatctgatcc tgttagatct
    38641 caatatgccc ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc
    38701 agggcgcatt gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa
    38761 acgcggcgcg gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt
    38821 gcatcaggca gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc
    38881 cagcttgcgc gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga
    38941 gcgcgatatt ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct
    39001 ggatatcacc gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct
    39061 caagtctcgc gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt
    39121 cccagcgcag ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct
    39181 cattggagga gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat
    39241 tgcttgcccc ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt
    39301 tggcttgtgc gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc
    39361 gaataccgta acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat
    39421 cccacggcgg tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg
    39481 gtagattatt tcgctgcgga ttgtcatagc gactaccacg taacgactga ctttcggcaa
    39541 cctcacccgc agaaagttgt tttttgagcg gtacaccaaa gcggtaatta agattcagcc
    39601 cgaggttatt ttgattctcg ccactttcac cctgtttatg ctgggccgtc acagtgacta
    39661 atggcacagg ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac
    39721 cagagttaaa caaatcaaca cgatcaccaa aatactgttc tacgctgaca ctggtattga
    39781 ggtgttgata gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt
    39841 gttcctgcgt ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata
    39901 gtcgcaaata ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca
    39961 gcaagttgtc ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc
    40021 caacgcccac attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg
    40081 tgagataacg atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat
    40141 gtccttcgtt atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact
    40201 ctacgtgctg attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt
    40261 ttgcctgctc gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca
    40321 caatttcagc aaagtgtttt tccccatcat gattttcggg agccatgcct aaatccggca
    40381 gaccatcatt gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt
    40441 tagctgtacc gcctgctaca agcaagagta aataaaacgg gataatgcga ggaacgaaac
    40501 ggctcaaaac gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga
    40561 ttactggcaa cttttgtcgt attttctcgc gctttcagga aaatccttaa aaatgtgacc
    40621 gattggtcag acgctgcaga agcagtaaga tagaagatga atgtctaatt ccctgaatac
    40681 aggagtaaat aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat
    40741 ccttgtttaa cagcttgcgg ctagccattg cgttacgaga gcaggagagc aatctggatc
    40801 tgcgtctgtt cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg
    40861 aaggctacaa cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat
    40921 tgtgcaaaac ctgtaccgac gggcgcggga ttagtacact tcctctgatt gatggggtgg
    40981 aaatcggtac tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat
    41041 tttaatgttt gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc
    41101 cggccgtaac acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa
    41161 atcattcagc ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag
    41221 gtattgtgcg ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg
    41281 agcgcgagtg tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt
    41341 tactgtgcat ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat
    41401 catctcgcgc ttccacaaca gggttagttc ctgctccagc gtctcttctg gcagtcgata
    41461 ggcgacgcct gtagtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc
    41521 agtcccgcgc ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc
    41581 ggtgcacgac tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat
    41641 ccacaccggt ccctcatcag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg
    41701 ctctgctgac cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt
    41761 catcaagaaa tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa
    41821 ctttttggtg tcttccggac atttttactg ctcattttga aaccactaac gggacaatat
    41881 gcaatccacg gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt
    41941 tttacgattt tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag
    42001 cccaggccac tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcgtctttat
    42061 ctttatattg atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg
    42121 gtagaacgtg ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact
    42181 ccttttacaa agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag
    42241 aattttcatg tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac
    42301 ttatttttat gtcccaatga gtaaaaaatc gacagtgggg taaaaattcg taaaatgtta
    42361 ccgatatcca gacactttct gttatttttc ctacctattt tttacatttt cgataaattc
    42421 atctgcaaag ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag
    42481 gagacttcgc ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc
    42541 cagacactac cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc
    42601 gcctttagtg ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt
    42661 tcgcttattc ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg
    42721 gcaaccggcg acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt
    42781 gttaccggtg ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa
    42841 tatatgaatc tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc
    42901 gtactggtat ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg
    42961 gtagcattaa tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat
    43021 caaagtttgt ttgtctacga gcacgaagat gacagtgatg acgatgaccc gcatcacggt
    43081 aaaccgtctg cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc
    43141 gttattgcag tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat
    43201 gcccctgtcg cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta
    43261 ggtgcgttaa aagcagtgtt gaacaaccag gtccagcgcg cgatgaatct gttctttggt
    43321 tcagtgttag caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg
    43381 ggtaacgaat tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta
    43441 gtgctgtgcc atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat
    43501 ctggcactgt ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga
    43561 tgacggaaat cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg
    43621 gacagctcgt taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca
    43681 gctgttgaaa agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg
    43741 aattgcg
//
LOCUS       DAZFKM010000025        42974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFKM010000025 DAZFKM010000000
VERSION     DAZFKM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..807
                     /gene="artJ"
                     /locus_tag="ACHKAY_001504"
     CDS             76..807
                     /gene="artJ"
                     /locus_tag="ACHKAY_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO5700203.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(982..2109)
                     /gene="rlmC"
                     /locus_tag="ACHKAY_001505"
     CDS             complement(982..2109)
                     /gene="rlmC"
                     /locus_tag="ACHKAY_001505"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO5700204.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2150..2638)
                     /gene="ybjO"
                     /locus_tag="ACHKAY_001506"
     CDS             complement(2150..2638)
                     /gene="ybjO"
                     /locus_tag="ACHKAY_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO5700205.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2698..3543)
                     /gene="potI"
                     /locus_tag="ACHKAY_001507"
     CDS             complement(2698..3543)
                     /gene="potI"
                     /locus_tag="ACHKAY_001507"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO5700206.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3540..4493)
                     /gene="potH"
                     /locus_tag="ACHKAY_001508"
     CDS             complement(3540..4493)
                     /gene="potH"
                     /locus_tag="ACHKAY_001508"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO5700207.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4503..5636)
                     /gene="potG"
                     /locus_tag="ACHKAY_001509"
     CDS             complement(4503..5636)
                     /gene="potG"
                     /locus_tag="ACHKAY_001509"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO5700208.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5731..6843)
                     /gene="potF"
                     /locus_tag="ACHKAY_001510"
     CDS             complement(5731..6843)
                     /gene="potF"
                     /locus_tag="ACHKAY_001510"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO5700209.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7194..7670)
                     /gene="ybjN"
                     /locus_tag="ACHKAY_001511"
     CDS             complement(7194..7670)
                     /gene="ybjN"
                     /locus_tag="ACHKAY_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO5700210.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7758..8660)
                     /gene="rimK"
                     /locus_tag="ACHKAY_001512"
     CDS             complement(7758..8660)
                     /gene="rimK"
                     /locus_tag="ACHKAY_001512"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO5700211.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8721..9443)
                     /gene="nfsA"
                     /locus_tag="ACHKAY_001513"
     CDS             complement(8721..9443)
                     /gene="nfsA"
                     /locus_tag="ACHKAY_001513"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO5700212.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9427..9714)
                     /gene="ybjC"
                     /locus_tag="ACHKAY_001514"
     CDS             complement(9427..9714)
                     /gene="ybjC"
                     /locus_tag="ACHKAY_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO5700213.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9874..10131
                     /gene="grxA"
                     /locus_tag="ACHKAY_001515"
     CDS             9874..10131
                     /gene="grxA"
                     /locus_tag="ACHKAY_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO5700214.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10161..10538)
                     /gene="ybjM"
                     /locus_tag="ACHKAY_001516"
     CDS             complement(10161..10538)
                     /gene="ybjM"
                     /locus_tag="ACHKAY_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO5700215.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10808..12493
                     /gene="ybjL"
                     /locus_tag="ACHKAY_001517"
     CDS             10808..12493
                     /gene="ybjL"
                     /locus_tag="ACHKAY_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO5700216.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12668..13204)
                     /gene="rcdA"
                     /locus_tag="ACHKAY_001518"
     CDS             complement(12668..13204)
                     /gene="rcdA"
                     /locus_tag="ACHKAY_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO5700217.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13288..14496
                     /gene="ybjJ"
                     /locus_tag="ACHKAY_001519"
     CDS             13288..14496
                     /gene="ybjJ"
                     /locus_tag="ACHKAY_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700218.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14496..15311
                     /gene="ybjI"
                     /locus_tag="ACHKAY_001520"
     CDS             14496..15311
                     /gene="ybjI"
                     /locus_tag="ACHKAY_001520"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO5700219.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15397..15681
                     /gene="ybjH"
                     /locus_tag="ACHKAY_001521"
     CDS             15397..15681
                     /gene="ybjH"
                     /locus_tag="ACHKAY_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO5700220.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15722..16954)
                     /gene="mdfA"
                     /locus_tag="ACHKAY_001522"
     CDS             complement(15722..16954)
                     /gene="mdfA"
                     /locus_tag="ACHKAY_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO5700221.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17239..17835
                     /gene="ybjG"
                     /locus_tag="ACHKAY_001523"
     CDS             17239..17835
                     /gene="ybjG"
                     /locus_tag="ACHKAY_001523"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO5700222.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17893..18651
                     /gene="deoR"
                     /locus_tag="ACHKAY_001524"
     CDS             17893..18651
                     /gene="deoR"
                     /locus_tag="ACHKAY_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO5700223.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(18698..19900)
                     /gene="dacC"
                     /locus_tag="ACHKAY_001525"
     CDS             complement(18698..19900)
                     /gene="dacC"
                     /locus_tag="ACHKAY_001525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO5700224.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20147..20773
                     /gene="gstB"
                     /locus_tag="ACHKAY_001526"
     CDS             20147..20773
                     /gene="gstB"
                     /locus_tag="ACHKAY_001526"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO5700225.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20770..21885)
                     /gene="yliI"
                     /locus_tag="ACHKAY_001527"
     CDS             complement(20770..21885)
                     /gene="yliI"
                     /locus_tag="ACHKAY_001527"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO5700226.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21996..22379)
                     /gene="bssR"
                     /locus_tag="ACHKAY_001528"
     CDS             complement(21996..22379)
                     /gene="bssR"
                     /locus_tag="ACHKAY_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO5700227.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22592..23917
                     /gene="rimO"
                     /locus_tag="ACHKAY_001529"
     CDS             22592..23917
                     /gene="rimO"
                     /locus_tag="ACHKAY_001529"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO5700228.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23964..25292)
                     /gene="dgcI"
                     /locus_tag="ACHKAY_001530"
     CDS             complement(23964..25292)
                     /gene="dgcI"
                     /locus_tag="ACHKAY_001530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5700229.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25300..27648)
                     /gene="pdeI"
                     /locus_tag="ACHKAY_001531"
     CDS             complement(25300..27648)
                     /gene="pdeI"
                     /locus_tag="ACHKAY_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5700230.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVLERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAVRGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(27826..28737)
                     /gene="gsiD"
                     /locus_tag="ACHKAY_001532"
     CDS             complement(27826..28737)
                     /gene="gsiD"
                     /locus_tag="ACHKAY_001532"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO5700231.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(28740..29660)
                     /gene="gsiC"
                     /locus_tag="ACHKAY_001533"
     CDS             complement(28740..29660)
                     /gene="gsiC"
                     /locus_tag="ACHKAY_001533"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO5700232.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(29678..31216)
                     /gene="gsiB"
                     /locus_tag="ACHKAY_001534"
     CDS             complement(29678..31216)
                     /gene="gsiB"
                     /locus_tag="ACHKAY_001534"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO5700233.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(31236..33107)
                     /gene="gsiA"
                     /locus_tag="ACHKAY_001535"
     CDS             complement(31236..33107)
                     /gene="gsiA"
                     /locus_tag="ACHKAY_001535"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO5700234.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(33094..34059)
                     /gene="iaaA"
                     /locus_tag="ACHKAY_001536"
     CDS             complement(33094..34059)
                     /gene="iaaA"
                     /locus_tag="ACHKAY_001536"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5700235.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            34263..35498
                     /gene="moeA"
                     /locus_tag="ACHKAY_001537"
     CDS             34263..35498
                     /gene="moeA"
                     /locus_tag="ACHKAY_001537"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO5700236.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            35498..36247
                     /gene="moeB"
                     /locus_tag="ACHKAY_001538"
     CDS             35498..36247
                     /gene="moeB"
                     /locus_tag="ACHKAY_001538"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO5700237.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(36323..36985)
                     /gene="fsa"
                     /locus_tag="ACHKAY_001539"
     CDS             complement(36323..36985)
                     /gene="fsa"
                     /locus_tag="ACHKAY_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5700238.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGMAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            37116..38015
                     /gene="ybiY"
                     /locus_tag="ACHKAY_001540"
     CDS             37116..38015
                     /gene="ybiY"
                     /locus_tag="ACHKAY_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO5700239.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            38021..40453
                     /gene="ybiW"
                     /locus_tag="ACHKAY_001541"
     CDS             38021..40453
                     /gene="ybiW"
                     /locus_tag="ACHKAY_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO5700240.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            40599..41414
                     /gene="ybiV"
                     /locus_tag="ACHKAY_001542"
     CDS             40599..41414
                     /gene="ybiV"
                     /locus_tag="ACHKAY_001542"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO5700241.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            41566..42831
                     /gene="ybiU"
                     /locus_tag="ACHKAY_001543"
     CDS             41566..42831
                     /gene="ybiU"
                     /locus_tag="ACHKAY_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO5700242.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT        10768 a        11357 c        10522 g        10327 t
ORIGIN      
        1 ttcacttgat ggcattgtta tcccatgccg cagacacggc caaaaatcat aagattgaca
       61 gacgggagtt ccatcatgaa aaagttagtt cttgccgctt tacttgcttc ctttactttc
      121 ggtgcttctg ccgcagagaa aatcaatttt ggcgtttcag ccacctatcc accctttgaa
      181 tctataggtg ctaataatga gattgtcggc tttgatatcg atctggcaaa agccttgtgc
      241 aaacaaatgc aggcagaatg tacttttact aatcacgcgt tcgacagcct gatcccgtca
      301 ctgaaattca gaaaatatga cgccgtaatc tccggtatgg atatcacgcc cgagcgtagc
      361 aagcaggtat cgtttaccac gccctactat gaaaactcag ccgtcgtgat tgccaaaaaa
      421 gatacctaca aaacgtttgc cgatctgaaa ggcaaacgta ttgggatgga aaacggcacc
      481 acgcaccaga aatatattca ggatcagcac cccgaagtga aaactgtctc ctatgacagt
      541 tatcagaatg cctttatcga tctgaaaaat ggtcgtattg atggggtatt tggtgacaca
      601 gcggtggtaa acgaatggct gaaaaccaat ccgcaactgg gcgttgctac tgaaaaagtg
      661 accgatccgc aatacttcgg caccggtctg ggcatcgctg tacgtccgga taacaaagct
      721 ctgctggaaa aactgaataa cgcgctggca gcaattaaag ctgacggtac atatcagaaa
      781 atcaatgacc agtggtttcc gcagtaattg agactgatga caaaagtaag attgcctgat
      841 gcgctgcgct tatccggccc acatcaactc tgcaatgtat tgatttagca tgattttgta
      901 ggccggataa ggcgtacacg ccgcatccgg catgaacaaa gcgcacctga tcagcaatct
      961 gaagccgcac ctgcggcttt tttattgctt caccagcagc gtcagcactt catagtgcgc
     1021 ggtgtgcggg aacatatcaa aaagctgtac ccgttcgatg cggtaacctg gcagttcgcg
     1081 gatatctttc gccatggttt gggcgttaca gctggagtag ataataaaac gcggtgccat
     1141 tgttgagaga taatcacata gcggtttacc aatgccgcgg cgcggcggat taaccagcac
     1201 cagctccggc acctccccct gagcggtggc aaactgagtg gagtccagcg cctgaaattg
     1261 caaacgcgtt aagcccagtt cagcggctga ctgctttgca caggcaatgg cctctgatgc
     1321 aatttcgatc ccggttaact gcatgtcagg cgtcgcgcag tgtaaaccaa agccacccac
     1381 accgcagaac agatcccaca tatgtttaac tggcagctgc cgtacccagt cgcgcgcggt
     1441 agcgtacagc tggctggcga ccgccggatt agtctggaag aaactttgcg gacggatcca
     1501 cagcggtacg tcattaaaac gctctgccag cgcctgttgt tcagtaaggt agatctccgt
     1561 ctccccttcc ataatcgcca tatgtaccgg ctgaatattg acggtaataa ctttcagttg
     1621 cggtagttgt tcctgtaacc acggcagcgc cttacgcagt tgtgccagtt tggtttcaga
     1681 acgcagtaca aagcgcagca tcatgccgcc atcgctctgg ctttcagtca gcagaatgta
     1741 tttcagttcg ccacgcttac gcgccacgtt gtagggtgtc agccccgcac gggcgataaa
     1801 cggttttagc gccgcaaaaa cgggcgcaaa tgaggcagga taaagcgggc agtcacaaag
     1861 gtcttctggt gtgccgtcgc gatgcagcat accgagcagt ggtttttcaa cgctaccact
     1921 caccaccatt ttggctttat tacgaaaccc ttgttccggg cctgacaccg gcgcgcacca
     1981 ttcctcaacc ggaaaatcgg cgagcagatt tttaagatcg gcggttttag cggagagttg
     2041 ctctggaatc ggctgcgtta tccactgaca ggaacgacag cgacccgcgt cgtaaagtgc
     2101 gcactgcata cattgacctt cacatcatca ggggcgacga ttatacacat tattgcaact
     2161 ggaagaaccg ccgactggtc gagggaacga acagcagcat caggatgagc atatccggca
     2221 gcttctgcag catcaggcta tggaagattt cacgttttga ttcaccggga atgctgaaca
     2281 gctccggata accgtacccc agcgaagccg cccacaagta acttgcggcg gtgatttgtg
     2341 tcagcagata tagccagcgc gcccagcgac gccctttcac cagtgaaaac gcacaccaga
     2401 tctcaatgaa aaccagcacc agactgctta aaaagaccag cgttaaactc caggtttgta
     2461 cgctgcgatg aatgaactcg ccaataccgc gcacgcccag cgtattgaaa atcatcagca
     2521 cgtcgaggcc acggatcata ataatggcga gcgccgccac ctgcaccagc gcaggcacat
     2581 tcaggcgagc atgagatgaa gatgttttct taaaaaatcc caacgtttcg tcttccatga
     2641 aaacaatgcc gcggcatgcg cggcattatg tagccaggtt ggcaaatttt agtgtcttca
     2701 gccatgtctt gcacgctgga tatcgcgtat ccgctgtttt tctgcgcgag ccatcagata
     2761 ccaggcgata aatccgacaa ttccgaccgc accgagaatt aacgttgcaa gggcgttgat
     2821 ttccggattc acccccatcc gcacgctgga aaagaccagc atcggtaacg tggtggctcc
     2881 cggcccggaa acaaagctgg cgatcaccag atcatcaagc gacaaagtaa aagccagtaa
     2941 ccagccagaa atgatcgcgg gcatgatcat cggtagcgta atgacgaaaa acactttcag
     3001 cggcgtcgca ccgagatcca tcgctgcttc ttctatcgag cgatccagtt cccgcaatct
     3061 tgaagaaata acgaccgcca cataagccgt acaaaaagtg acatgcgcca gccagatggt
     3121 gagcataccg cggtccgcag gccagccaat ggcatgagca agcgcgacga ataataacaa
     3181 cagcgacaag cccgtgatga catctggcat caccagcggc gcggtgatca taaaggcaaa
     3241 gccatttgat ccgcgaaacc tgccaaagcg caccagcacc accgccgcaa tagtcccgag
     3301 gatcgccgcc gccgttgccg cacaggcagc aattgtcagg cttaaaccaa ccgcactcat
     3361 catcgcgtca tcgcgcaata actcaccata ccagcgcgtt gaccagccgg cccacaccgt
     3421 caccagcttc gagctgttaa acgaatagat aaccagcatc agcattggcg cgtagagaaa
     3481 ggtaaagccc agcagcaaaa tcacaatccg ccagggcgaa cgaactaccg gtaaattatt
     3541 catccgtgtt ctcccacgct tttttgctgg tgtttgtgga accacattat cggcacaatt
     3601 agcagcagca acatgatgat cgctaccgcc gaggccaccg gccagtcgcg gttattgaaa
     3661 aactcttgcc atagcacgcg cccgatcatg atgctgtccg ggccaccgag cagttccggg
     3721 atcacaaact cgcccacagc cgggataaac accagcatcg atccggcaat aatcccacct
     3781 ttagtcagcg gcacgatcac agtaaagaac gttttcagcg gtcgtgcacc gagatccagc
     3841 gctgcttcca ccagcgaata atcaatacga atcaacgcgg tataaatcgg cagtaccata
     3901 aacggcacgt aggcgtaaac aatgccaata taaactgcca gattggtatg aagaatggtc
     3961 agcggttgat cgataacccc cagccacagc agaaaattat tcagcacacc gttgtttttt
     4021 aatattccca tccaggcata aacgcggatc agaaacgagg tccacgacgg caggatcacc
     4081 agtagtaata aaatattacg ggtcgaaggc ttactgtgcg ccaccgccca cgccagcgga
     4141 tagccgatca gtaaacagca aaatgtcgaa atcgcagcca cctgtagcga ctggagataa
     4201 gcatcgaaat agagcggatc gtcggtcagt tgcagaaaat taccgagatt aagagtgatg
     4261 gacagttgcc cgtcagccca ctccatcagt tcggtataag gtggaatagc gcgcgccatc
     4321 tccgccaggc ttattttaaa gacaatcaga aatggcagca gaaacagcaa gatcaaccag
     4381 atatatggca acgcaatgac cagtttgcgc ccatgcttca tttgcagctg cgacaaccac
     4441 agcttaaatc cgcccggcgg tttcgactgg gcagcaggtt caagtgtatt catctttgct
     4501 ccttaaaccg tcagcaccac acagctgtcc acttcccagc acaaacgcac ttcgtcgccc
     4561 caggtcggta accctttacg atgacgatgg gcgttttgta gctgggcgct aatcatctgc
     4621 ccacttttca gacgaacgtg atacaccgaa agatcgccga gataggcaat gtgtatcacc
     4681 tcccccaccg cgaagttaca accattggcg ggcggctctt cgcaaagcat gattttttcc
     4741 gggcgcagcg ctacgtgtac cggcacgtta tcgaccactg aagcatccgc atcgactttc
     4801 agtggatgca ccagccccgg cgaatcaagc accaggccat cttcctgacg ctctttgagt
     4861 acaccttcaa agacattaac cgaaccgata aattcagcgc tgtagcgggt agttggatgc
     4921 tcgtagatct cttccggctc gccaatctgg acaaatttcc cgcgattcat aatggcgata
     4981 cgccccgcca tggtcatcgc ctcttcctga tcgtgggtga ccatcacaca agtcacaccg
     5041 acgcgctcca gaatatccac cacttcaagc tgcatccggt cacgcagctt tttatccagc
     5101 gcgcccatcg gctcatcgag cagtaatagt ttcgggcgtt tggcaaggct tcgggccagg
     5161 gccacacgct gtcgctgacc accggaaagc tgatgcggtt tgcgtttggc gaactcctgc
     5221 atgtgcacca gcccaagcat ctcgttgacc cgggtggcaa tttccgcttt cggtagtttg
     5281 tcctgtttca ggccaaaggc gatgttctgc tccacagtca tatggggaaa cagcgcgtaa
     5341 gactgaaaca tcatattgat ggggcgcagg taaggcggca cctgtgacaa atcgacgcca
     5401 tcaagcatta tctgtccggc agaaggttgt tcgaaacctg ccagcatacg cagcagcgtg
     5461 gacttgccac agccggatgc gcccagcagc gcgaagattt cacctttgta gatggtcagg
     5521 ctgacatcat ccaccgcatg ttgaccatcg taggatttgg tgaggttgcg gatttctaat
     5581 agcggcgtca gcgccttacg ggttttcgcc tgcgggcgag ggatagcgtc attcacggct
     5641 gctctccggc attgaatgtg tgcaagcgca ccatgatggt gcgaattgcc ggcgggtgtg
     5701 gtgcgcccgc ccggcatcta aggctgcgga ttattttccg ctcttcactt tggtccacgc
     5761 gcgggtgcgc acacggtcga ttttcgggtc ctgcactttc agagtgaaca gcttcgcacg
     5821 aacatccgca ggcggataaa tacccgggtt ctcacggact tccgcactca ccagcggcgt
     5881 ggctgcttta ttggcgttgg catagaacac attgtcggaa atatgcgcta ctacatccgg
     5941 gcgcagcagg taattcagga actgataggc ttcgtctttg tttttggcat ccgcaggcat
     6001 ggcgaataca tcaaagaacg ccatcgcccc ttcttttgga atcgagaacg agacattcac
     6061 tccattcttc gcttccttcg cgcggtttga cgcctgccag acatcacctg cccagccgat
     6121 agcgacgcaa atatcgccgt ttgccaggtc gttaatgtat tgagatgaat ggaaataacg
     6181 aatgttcggg cgcagcttta acagcagatc ggttgccggt ccggtgtaat catccgcttt
     6241 agtgctgttg ggatctttgc cgagataatt caacacggta gcaaaaactt cttctggcgc
     6301 atccaggaaa gagacaccgc agcttttcag tttttccaga ttttcaggtt tgaggatcaa
     6361 gtcccagcta tcgacgggcg cgttttcgcc cagcacagct ttaactttat caacgttata
     6421 gccaatcccg gtcgtcgccc acatataggg catagcaaat ttattgtcgg gatcgtgttt
     6481 ggcgaccagc ttcagcagct ccgggtcgag attcttccac tccggcaatt tgcttttgtc
     6541 cagcggctgg aaaactcccg cagtcaactg gcgttcaaga aagctggcag atggaaccac
     6601 caagtcaaag ccggtgctac ccgccattaa tttgccttcc agtacttcgt tagagtcgaa
     6661 aacatcgtag acgactttaa taccggtttc tttttcaaaa ttggccaccg tgtccggggc
     6721 gatataatca gaccagttat aaacgtggag tgttttttgt tcagccgcga gcgtgccgac
     6781 agagacggcc atcagagcac ccgcagccag acccgaaagc cattttttat ttaaggcggt
     6841 catatctctt tccttctgaa agttcgttaa caaacgaaca aaagttgtca cactaaaaga
     6901 tatgcaataa acgtgcatat tttttatcgg cggggtgtaa gaaaagagaa ctctctcccg
     6961 gccagcactg ctcgctatat aaaagcatcg caagaataac tttagccagg gtttgagact
     7021 atagctcgga ttaaaaaacg gtatttaaca aatttttatg cgtatttgct ctatgtgata
     7081 aagccaaatc gacacaagag aagtgaaata atctttaaga aaaggttaaa aacagcagat
     7141 aattttgcag cgcgcagcca ctgcaccagc gactgcgtgc tcgaaaggag ttatcagtgg
     7201 aggaaatagt tttcggtaac gttgttttgc ccttcatcgt cagtcggtaa cagcagttga
     7261 tgggcattag cttcaagaat gaccatcgaa atctgctctt cgctctgacg cacgaaccat
     7321 gcaaactgct cataggttac gccctgcata acggataaag actggcaaac caccagcttt
     7381 ggcagattat catcctgcat atcaagaaat gctttcacgg tcagcgaact ggcattgatg
     7441 gcagataaat ccgccgccag cggcaatacg gctgaaggtc ggacttccgc catggcagaa
     7501 aacaggatcg tgttatcgat cagatcgatt ttggcatcaa agacaccgtc gaaattctgc
     7561 atatggggca gatgcagagc ctgacagtta tcacattcaa aaaaactcat ccccaggtca
     7621 tcgagccatt gacgcagcgt atccagacca ggaacgacca gcgatgtcat aattttgtac
     7681 aacctctttc gataaaaaga ccggcacagc ttacgcaaaa agcgcaggca aaaccatgat
     7741 cagtaatgtg attgcgacta accacccgtt ttcaggcaat attctgtcgt agcgtggcgt
     7801 tcgatccagc ggatcatttt acccgcgatg tcgataccgg tggttttttc tattccttcc
     7861 agccccggcg acgcattcac ctccatcacc aacggcccgc gattagcacg cagaatatcc
     7921 acaccagcaa cgtccagcgc catcgttcgc gcggctttta tcgcgatttc acgctcctgt
     7981 ggtgtgatac tggcgacgct tgccgcgccg ccacgatgca aattggaacg aaaatcgccc
     8041 tctttcgccc gccgttcaat cgcagcgacc acttcatcgc caacaaccag acagcggata
     8101 tcgcaccctt gcgcctcttt gatatattcc tgcaccagaa tatgcgcgtt cagaccgcgg
     8161 aaagcgtcaa tcacgctttc cgccgcctga cgcgtctccg ccagcacgac gccaattccc
     8221 tgcgtgcctt caaccaactt gaccaccagc ggcgcaccac cgaccatgtc gattaaatcg
     8281 ctggtatcat ccggcgaatg cgcaatgccc gtgacaggca ggtcgatgcc ctgacgcgcc
     8341 agcagttgca tggaacgcaa tttgtcacgc gcccgggcaa tggcgaccga ctcattgagc
     8401 ggatagctcc ccagcatctc gaactggcgc agtgccgccg tcccataaaa ggtaatggcg
     8461 gtgccaatac gcgggatcac tgcgtcaaaa tggggtaact tgcggccttt gtagtgaata
     8521 gaagacgccg caggatttat gttcatgtag caagaaagcg gatcaagaat ttcaaccagg
     8581 tgaccgcgct gtatagcggc ttcacgcagc cgcttacacg aatagagcgt tccatcccgg
     8641 gacaatatgg caattttcac cctgcacctc tctgtcagac ctggtcaaaa gcccgcgtat
     8701 catacatcga cgtggcggtt ttagcgcgtc gcccaaccct gtttgtgcaa ataatccaga
     8761 ataaatgggc ggctttcttt aatgattgtt cggcggatat gatcgctcca ggtatcccgg
     8821 cgattattgc tgccacgggt gaggtaatat tccgccagtt gctcgtcata ctgcgccagt
     8881 gcgcctttat ccagcggttg atagctgttt tcatgcacca aaatggaggc cggtaaacgc
     8941 ggcttaagat ccggattatc cgcaggccag ccaaggcaca gcccaaacag cggcagaaca
     9001 tgctgcggta atttaagcag tttcgtcacc gcttcaatat tattgcgcag gccgccgata
     9061 tataccccgc ccaatcccag cgattccgct gcgattaatg cattctgcgc catcattgcc
     9121 gtatcaacga caccgagcaa cagttgttcc gccaggccga gctgagcatc cggacagatc
     9181 tgtaaatggc ggttaaagtc ggcacagaac acccagaact ccgccgcttg cgctacgtgt
     9241 ttttgcccgc cggtcagcgt caccagttct tcacgtaacg ctttgtcggt aatgcgaata
     9301 atgctactgc actgcaaaaa actggaactg gacgtcgcac gggcgctgtt aataatcgcc
     9361 tcacgctgcg cttcggaaat gggttcatca gtgaaatggc gaatggagcg atggccacaa
     9421 ataagttcaa tggttggcgt cattatcttt ttctctttct gaacgtgaat attgcggtgg
     9481 acggttcatc agctgtgggg caagacgttt tgccacctga agaataacca ccaccgcagc
     9541 gggaagcatg agcaaaacac cgagaaaaat catcagaatc tgcacttctg gccgagaaaa
     9601 tggctcaggc agcgacaggg agtcgcttac cgacagcagc gccaccgcca gtagcatcat
     9661 tccgataaat tccagtatca acacgccttt aggcaattta ccgatcgcgc gcatacgctt
     9721 ccctctgcaa agtgagcctt cagtctaaaa cttttcactg tattgtgttt aacagttata
     9781 gcttttagca attaatgcaa caggttaaac ctactttcag cgaatacatt ttagcgtgat
     9841 cattacaggc ataaatctat gaggagagaa ataatgcaaa ccgttatttt tggtcgttcg
     9901 ggttgccctt actgtgtgcg tgcaaaagat ctggctgaga aattgagcaa tgaacgcgat
     9961 gattttcagt atcagtatgt agatattcgt gcggaaggga tcactaaaga agatctacaa
    10021 caaaaggcag gtaaacccgt agaaaccgtg ccgcagattt ttgtcgatca gcaacatatc
    10081 ggcggctata ccgattttgc tgcatgggtg aaagaaaatc tggacgcctg atcgtctgac
    10141 aagccctcgc gttgagggct ttactgattt tttctgtgct gtggtttaaa caaactactg
    10201 ataaataaga aacacagtgc ccccagcgca caccagaaca ccgcgcttag taaccatgcc
    10261 agctcttgcc agaatgagcg cgtcggtgaa aaaaacagcc gcataatgag cattgaacag
    10321 ggtgccgcca gcattgcgcc aaatagaggt ttcaggactt ctctacgctg tgaaaagaag
    10381 ctggcgactg ctccaggaag aatgaaaaat agcaagccga tttcaggatg cccggcagcc
    10441 cgaaaagcgc ctttcatgtg cgtcgccaga aaaaggcaca ccacaatgaa gaggacaaaa
    10501 cagcagattg cccccgccca acgttgttta tgtttcactc gttcctcctg acactgcgtc
    10561 tatcgaacac atttttcgcc agtgtggcgt tcagtaagat aaagccgctt cgcattccat
    10621 gctaatatag gccaacgcaa ttcatatagc cgttgatacc taatgtgatt acactagtaa
    10681 aatatattgt tactttacta tcgtttaggt gcgctgaatg aatctgcgcc ctgaattctg
    10741 gtaaaaaaca ttatcgtaaa ttaccatttc tttcaacagc ttactagtaa acaagaagtt
    10801 agcctccgtg aatataaacg tcgccgaatt gttaaatggg aattacattc tgttattatt
    10861 tgtggtcctc gcgcttgggc tatgtctcgg aaagttacga cttggttcga tccaactggg
    10921 taattccatt ggcgttttag tcgtatcgct gttattaggc caacaacatt tcagcattaa
    10981 caccgatgcg cttaatcttg gctttatgct gtttattttc tgcgtcgggg tcgaagccgg
    11041 accgaacttt ttttccattt tttttcgcga tgggaaaaat tacctaatgt tagcactggt
    11101 gatggttggc agtgcgctgg tgatcgcctt agggttaggt aagctgtttg gctgggatat
    11161 tggcctgacg gccggtatgt tagcaggctc tatgacgtcg acaccggttc tggtcggtgc
    11221 tggcgataca ctgcgtcatt ccggcatgga aagcaggcag ctctcactgg cactggataa
    11281 tctgagcctc gggtatgcct taacctattt aatcggtctg gtgagtttga ttgttggtgc
    11341 gcgttacttg ccgaaattgc agcatcagga cttacagacc agcgcccagc aaatcgcccg
    11401 cgaacgtggc ctggacactg atgccaaccg taaggtttat ttaccggtga tccgcgccta
    11461 tcgcgtcggc ccggagctgg tggcctggac cgacggcaaa aatctgcgtg aactgggtat
    11521 ttatcgacaa accggctgct acattgaacg tattcgacgt aacgggattc tggcaaatcc
    11581 agacggtgat gccgtgctac aaatgggcga tgaaatagcg ttggtaggct atcccgacgc
    11641 ccatgcccga ctcgatccca gcttccgtaa cggtaaagaa gttttcgatc gtgaccttct
    11701 cgacatgcgt atcgtcactg aagaagtggt cgttaaaaac cataacgctg taggtaaacg
    11761 tctcgcacaa ctgaagttga ccgatcacgg ttgcttcctt aaccgcgtca ttcgtagcca
    11821 gattgagatg ccgatagatg acaacgtcgt gcttaacaaa ggtgacgttt tacaagtcag
    11881 cggtgatgcc cgtcgcgtaa aaaccatcgc cgatcgcatc ggctttatct cgattcacag
    11941 ccaggtcact gacctgctgg cattttgcgc cttctttgtt attgggctga tgatcgggat
    12001 gatcaccttc cagttcagca cattcagttt cggcatgggg aacgctgccg ggttgttatt
    12061 cgccggaatt atgctgggct ttatgcgtgc taaccacccg accttcggtt acattccgca
    12121 aggtgcatta agcatggtga aagagttcgg cttgatggtg tttatggcag gcgttggtct
    12181 gagcgccggt agcggtatta ataacggcct gggcgcgatt ggcggtcaga tgttgattgc
    12241 cggattaatt gtcagtcttg tgcccgtggt tatctgtttc ttgttcggtg cttatgtatt
    12301 gcgaatgaac cgcgcactgt tgttcggcgc aatgatgggc gcacgcacct gcgcgccggc
    12361 aatggagatc atcagtgata cagctcgcag taacatccct gcgctgggct atgcgggcac
    12421 ctacgcaatc gccaacgtcc tgctgacgct ggcagggaca atcatcgtca tggtatggcc
    12481 aggattagga taaaactgaa gttgccctga aaatgaaatt tttttgcaca accgcagaac
    12541 ttttccgcag ggcatcagtc ttaattagtg ccactgcttt tctttgatgt ccccattttg
    12601 tggagcccat caaccccgcc atttcggttc aaggttgatg ggttttttgt tatctaaaat
    12661 ttatctacta ccctgcaacc ctctcaacca tcctcaaaat ctcctcgcgc gatagcggct
    12721 tacggtcggt gacaaaatgc agcgtcatcc cctcaataaa cgcatcaagc gcgcgggcgg
    12781 ttccgggttc aaaccattgt tcgagcgttt gctgactgcg ctgcatccag ttttgcatta
    12841 ccgtttttaa tagcggtttt cggctagcca gcgcgtagag ctggtacatc agctccatgt
    12901 tatccggcgt tgcaacctgt gagctgtaga tcatatcggt gatagcctgg catgcgcctt
    12961 gagcatcact aacatcgcta aaaaatgcct gatattgccg ggacataatc tcagtaaaac
    13021 tgctgaacgc ctccagcaac aactcatcaa ttcctgaaaa atagtaggtc atcgatccca
    13081 acggtacccc ggcaagggta gcaattttgc ggtgcgtaac agcatgtatt ccgtaaagtt
    13141 tcaccgcctc cagcgtggcc tggataattt tttctcgccg ttgcggatcg ttagcgcgac
    13201 gcatgtcatt tcctctgtct gcaattgtgt acaaatgtac acaaccttgc taatgttgtc
    13261 ttccctcttc ttattttgac gtgatctatg accgtaaatt cttcacgtaa tgcattgaaa
    13321 cgccgaacct gggcgctgtt tatgttcttc tttttgccgg gcctgttaat ggcgtcctgg
    13381 gcaacccgta cgcctgctat ccgcgatatt ctctctgtct cgatcgctga aatgggcggt
    13441 gttctctttg gtctgtcgat cggttcgatg agcggtattc tctgctcggc gtggttagtg
    13501 aaacgctttg ggacacgtaa tgtcatcctg gtcacgatgt cctgcgcatt gatcgggatg
    13561 atgatattaa gtctggcact ctggctgaca tcacccctgc tcttcgcggt tggcctcggc
    13621 gtctttgggg caagttttgg ttctgcggaa gtggcgataa acgtcgaagg tgccgccgtt
    13681 gagcgagaaa tgaataaaac ggttttgccg atgatgcacg gtttttatag cctgggcacg
    13741 ctggcaggcg ctggtgtcgg gatggcactg acggcctttg gcgttccggc aacggtgcac
    13801 attttattgg cggcgctggt aggaatcgcg cctatttata tcgccattca ggcaatccct
    13861 gacggtacgg gcaaaaatgc tgccgatggc acccagcatg gtgaaaaagg cgtacctttt
    13921 tatcgcgata tccagttgct gctgattggt gttgtggtgc tggcgatggc ctttgccgaa
    13981 ggttctgcca acgactggtt acccttatta atggttgacg gtcacggttt tagccctact
    14041 tccggctcgc tgatttatgc cggttttacc ctggggatga ctgttggacg ctttaccggc
    14101 ggttggttca tcgaccgtta cagtcgcgtt gccgtggttc gggccagtgc actaatggga
    14161 gcgttgggta ttgggctgat tatttttgtc gatagcgcct gggtcgctgg ggtgtctgtt
    14221 gtactctggg gactgggtgc ctcgctgggc ttcccgctga ccatttctgc cgccagcgat
    14281 accggccccg atgcaccaac ccgcgtcagt gtggtagcaa cgaccggtta tctggctttc
    14341 ctcgtcgggc cgccgctgct gggctatctc ggcgaacatt atggattacg tagtgcaatg
    14401 ctggttgtac tggcgctggt tattctcgcg gctattgtcg cgaaagccgt cgccaaaccc
    14461 gataccaaaa cgcagacggc gatggagaat agttgatgag cattaaatta attgcggtag
    14521 acatggatgg tactttctta agcgatcaaa aaacctataa ccgtgagcgg tttatggctc
    14581 agtatcagca aatgaaagca caaggcattc gctttgtggt cgccagcggg aatcaatatt
    14641 atcagttgat ctctttcttc cctgaaattg cgaatgaaat tgcctttgtg gctgaaaacg
    14701 gcggctgggt agtgagtgaa ggcaaagatg tttttaatgg cgagctgtcg aaggatgcgt
    14761 ttgctactgt cgtggaacat ttgctgacgc gcccggaagt ggaaattatt gcctgcggaa
    14821 aaaatagcgc ctatacactc aaaaaatatg acgatgccat gaaaacggtg gcggaaatgt
    14881 attatcaccg tctggaatac gtcgataact ttgacaactt agaagatatt ttctttaagt
    14941 ttggcctgaa tctttccgat gaactgattc cacaagtaca aaaagcatta catgaggcca
    15001 tcggcgatat tatggtgcca gtccacaccg gcaacggcag catcgatctg attatccccg
    15061 gcgtacataa agccaatggc cttcgccaac tgcagaaatt atggggaata gacgacagcg
    15121 aagtggtggt ctttggcgat ggcggtaacg atattgagat gctgcgtcag gcaggcttta
    15181 gttttgcaat ggaaaatgcc ggcagcgcgg tcgtcgcagc ggcaaaatac cgcgcaggct
    15241 ccaataaccg tgaaggcgta ctggatgtga tcgataaagt tcttaaacac gaagcgccat
    15301 ttgaccaata aaaacggcag cacgcccggc aacacaatgc ctgaatgaag gctgcataat
    15361 tcgcggaaat taacccgctg cattatttca ggcgaaatga ttatgaaaaa ttgtctactg
    15421 ttgggcgcgc ttttaatggg ctttactggt gtggcgatgg cgcaaagtgt caccgtcgat
    15481 gtgccgagtg gatacaaagt ggttgtggtt cctgattcag tcagcgttcc acaagcggtc
    15541 agcgtcgcga ctgtgccgca aaccgtctat gtggctcctg cgccagctcc ggcttaccgc
    15601 ccgcatcctt atgttcgcca tcttgccagc gtaggtgaag ggatggttat cgaacaccag
    15661 attgacgacc atcaccatta attcgagaat gcctgatcgc acaaagcaat caggcatttt
    15721 tttacccttc gtgagaattt cccatctgtt tatcttttaa aaagataacc atcagcgaca
    15781 gccacaaaat tccgttgaca aggttgaaga gattaaacag tccgttgccc ccgttcagcc
    15841 aggcatgttt gctgatttca ataccgacgg taaagatcag catttgcagc attcccatcg
    15901 ccgcagaaac cgtaccttta ctcatatcgc tggcaaacag ggttaatcgc accagtcccg
    15961 cattcgccag accaatgccg aaagcataaa tacttaaccc ggcggtcatc cataaatacg
    16021 cgtgcgatga gataaccgtt gccgcagcag cgaccaacag accaatcata atcggccagc
    16081 cgcccataat tatcagcgaa cgtacggtgc ggcgcgaggt caaacgcgct aacaacaagt
    16141 tacccgcaat taacgccccg aaaataggca cttgcagcaa gccatattca tagctgctca
    16201 actgctcgcc ggtaatgatg ataatcggcg actgggcgat ccacgccagc aatggcaggc
    16261 taacgaatcc cagcgccagc gcccccgcca caaagcggcc gttcttcagc accagcttat
    16321 agtcacgacc gagttctttc agtgacagtt tctcgcctat acgcgtggcg gtttcaggca
    16381 tggcttgttg cagaccgaaa aaggagatcg ctgccaatgc ggcaaacaag acaaacatcc
    16441 cttcccaggg cagcacatgg atccacgccg cgcccaccag cggaccaagt agcggagcaa
    16501 tcagcgccac gttcgccatc agcgcggtga tcttgataca aaccgcctct tcgaaggatt
    16561 cctgaattgc ggcgtatccc acagcgccaa tgaaacagag gcttatgccc tgcaagaagc
    16621 gcaacagagt gaattgttca atgttttgcg ccagtaatat tgccagacag gtgatgataa
    16681 accacaccac ccccgccagc atcaccggac ggcgaccaat acgatccgac agcggcccca
    16741 ggagccattg taaaaacatc ccgcccgcca gatatgcggt cattgaagta ggaacccaat
    16801 caatgcccgc ctgatattgt tccaccacgg ccaacatacc gggttgaatc atatcgttgc
    16861 cgatataggt tgaaaattcg taaagcacca gacagagagg gaaaagtaac gcctgacgtc
    16921 caagcctggc accggaagct aatttatttt gcatgcaatt tcttcgccaa taataatcgc
    16981 gcagagttta ataaaagcac agctaacgag aaagcgaatt ttgtagctga aaccacggtt
    17041 aagcacattc ttacattatt gcgagtatag ctacgctttc tttaagtttt atttaaccta
    17101 tgcccgttac aattacccac cgtaaacagg ccgcttgagg gaaataagac gatgccgctt
    17161 tacccagttt aacctgcact ttattctcaa cgacttgcct gtattggctc ccttttaatc
    17221 actttgcgtc gggaagttat gctggaaaat ttgaatctct ctctattctc tcttattaac
    17281 gcgacgccag actcggctcc gtggatgatc tcgttggcga tttttattgc taaagatttg
    17341 attaccgtgg taccgctgct ggccgcggtg ctttggttgt gggggcttac agcacaacgg
    17401 caactggtga taaaaatcgc tatcgcgctg gcggtcagcc tgtttgtgtc ctggacgatg
    17461 ggacatcttt ttccgcacga ccgacccttt gtcgaaaata tcggctataa cttcctgcat
    17521 catgcggcgg atgactcatt cccaagcgac cacggtacgg tgattttcac ctttgcactg
    17581 gcatttttat gctggcatcg cctgtggtcc ggctcacttt taatggtgct ggccgtcgtc
    17641 attgcctggt cgcgcgttta tcttggcgtg cactggccgc tggatatgct cggtggattg
    17701 ctggctggta tgattggctg ccttagcgct cagattatct ggcaagcgat ggggcataaa
    17761 ctctatcaac gtctgcaatc gtggtatcgc ttctgttttg cattaccgat ccgcaaaggc
    17821 tgggtgcgtg actgatctct gctaaaaagt gtagtattga gcggctcgct tcaataacta
    17881 ttcagaggga ttatggaaac acgtcgcgaa gagcgcatcg ggcagctgct gcaagaatta
    17941 aaacgcagcg ataagttaca tcttaaagac gccgccgccc tgcttggggt ttcggagatg
    18001 acgattcgtc gcgatctgaa caaccacagt gcgcccgtcg ttttgctcgg cggctatatt
    18061 gttctggaac cgcgcagtgc cagccattac ctgttaagcg atcaaaaatc ccgcctggtg
    18121 gaagaaaaac gccgggcggc aaaaatggct gcgacactgg tagaacccga tcagactctc
    18181 ttttttgact gtggcaccac cacgccgtgg attattgaag cgattgataa tgaaatccct
    18241 tttaccgccg tttgttattc gctaaatacc tttctggcgc tgaaagagaa accccattgc
    18301 cgcgcatttc tgtgcggtgg tgaatttcac gccagcaacg ccattttcaa acccatcgat
    18361 tttcagcaaa cgttgaataa tttttgcccg gatatcgctt tttattccgc ggcgggcgtg
    18421 catgtcagta aaggcgctac ctgttttaat cttgaagagt tgccggtaaa acactgggcc
    18481 atgtcgatgg cgcaaaagca tgtgctggtt gtcgaccaca gtaaatttgg caaggtgcgt
    18541 ccggcgcgca tgggtgacct gaaacgcttt gatattgtgg tgagtgattg ttgcccggaa
    18601 gatgagtatg tgaagtacgc gcagacgcac cgtattaagt tgatgtatta atgacgtata
    18661 accggatgac gtttcgcgcc atccggttat cagaagatta agagaaccag ctgccgaacc
    18721 actgatggaa tttcatcatc acgaaatccc acatccgacc aaagaatccg ccctcttcca
    18781 cattttccat cacgatcagc ggacgctgct caatggattt accgttaagc tggaaatcaa
    18841 tggtcccgac aacctgacct tttttcagcg gtgcggtaag ctgcggttcc gttaacgtgt
    18901 agctcgcctt caggtttttc agctgcccac gcggtatggt cactgagccc gcttcgcctg
    18961 ccccaagatt cacttcgctc ttatcaccaa accagacgcg ctgagtcaca aaggtggcat
    19021 caggtttaat tggcgtcacg gtttcaaaga agcggaaacc ccaggtcaat aatttctcag
    19081 actcattaaa acggatacgg tcagttttcg cccccagcac tacggagatt aaacgcatat
    19141 cgccctgggt agccgaagca accagattat atcccgcgcc tgccgtggtt cctgtcttca
    19201 tgccatcaac attcagattg ctgctccaca gcagacggtt acggttaggc tgacgaattt
    19261 tgttgaaggt gaactctttc tctttatgaa tggcgtactc ttccggcaca tcgtggatca
    19321 atgctttacc cagcaatgcc atatcgcgcg cggtactgaa ctgccccggc gcatccagac
    19381 cgtgcaccgt ctggaaggta gtgttggtca gacccagttt tttggcataa ccattcatca
    19441 aaccaatgaa tgactcctgg ctcccggcaa cgtaatcggc cagcgcaata caggcgtcat
    19501 taccggactg gataatcaca cctttgttca agtctgccac cgaaacctga tcgcccggtt
    19561 tgaggaacat taccgatgaa ccacgcagtg ccggatttcc cgtcgcccag gcatctttac
    19621 cgaccgtcac catatcggtg agtttaatct tatcggcctt aagcgcctgc ccaaccacat
    19681 agctggtcat gattttagtc aggctcgcgg gatccagttt ctcatccgcg ttgccttctg
    19741 ccagcacttt accgctggcg taatccatta aaatccatgc acgcgcatcc acgctcggcg
    19801 cttcaacggt ttgttccgcc gcgaatgccg ttggggcaaa aaggaataaa aatgcagaac
    19861 ccgctgcaag accacgaagg agagaggagt attgcgtcat aaatgccacc cgagtatcca
    19921 ttcaaaaaaa atacgttata tcgccgtatg ataagttcgg tgattaataa cgcacacaca
    19981 aacttaaaga aaccgtaagt tggtaaagtt tttaaagttt atgcaatatc ctgccgcttc
    20041 gctgcttctg gtgtcatttt tttgccagct gttgcttaaa agttaacttt acttcaccat
    20101 cggagtcctg ttcagcctgt ggctgcctgt cggaaaaggg gtgagtatga ttacgctgtg
    20161 gggtcggaat aattcaacca acgtaaaaaa agtattgctg acgctcgaag aactggaact
    20221 accttatgag caaattctcg cgggccgtga gtttgggata aatcacgatg ctgattttct
    20281 ggcgatgaac cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg atctcattct
    20341 ttgggaatca aacgccattg tccgctatct ggcggcacag tacgggcaaa aacgtctgtg
    20401 gatcgactca ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg caaaccagac
    20461 gctcagtaat gctcatcgcg ggatcctgat gggattagtc agaacaccac cggaagagcg
    20521 cgatcaggcc gccattgatg ccagttgcaa agagtgcgac gccctgtttg ccctgctcga
    20581 tgcggaactg gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg gcgatatcgc
    20641 tatcgcaccg ttcatctaca atttgtttaa cgttggcctg acctggacac cgcgtccaaa
    20701 tctgcaacgc tggtatcagc aactcactga acgccccgca gtgcgcaaag tggtgatgat
    20761 tcccgttagc taattgcgtg ggctaacttt aagtaattcc ccactggact cgtcggtgag
    20821 aacgtataaa taaccgtcgg gtccagtgcg aacatcacga attcgctgcc ctctgtccgt
    20881 taaaatacgg ccatcttctg tcactttgtc gccgttgacg ctcatcacaa tgacatcttt
    20941 atctttcagc gcgccaataa ataatttttg ctgccactgg gggaatttat cgctgttata
    21001 gaaggccatg ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt gctcggtccc
    21061 ggcgacgatc tcccctttcg cttccggtat cttaaagcct gaatagttga ttccccaggt
    21121 tgccagcggc cagccgtagt ttttgccttt ttgcgggata ttaatttcat caccaccgcg
    21181 cgggccatgt tcattcagcc acagtgcatt actccacgga ttcattgcca ttccttgcgg
    21241 attacgaatg ccataagacc agatctcggc gcgcacaccg gattccttta taaaaggatt
    21301 atcatccggg atttcgccct ggtcggtcag acgcaccagt ttgccctgta atttatccag
    21361 atcctgcgcc gtcgggcgct gattgttttc gcccagagca ataaaaagat aacctttacc
    21421 gtcgaatacc agccgcccgc caaaatggtt gccggtagac agttttggca tctggcggaa
    21481 gacggtgcgg aagtcggtca cttttgagag atcatcactt aagcggccat aacccacagc
    21541 agttccggct ttgccatcat cgccaacttc ggaataactt aaccagatgc ggcgagactg
    21601 agcaaaatca ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg cccaaacgtc
    21661 cggaactccg gaaagcggcg cagataatcc ttttcctgct tgccagtgac gcaactcgcc
    21721 gccgcgcaga gtgattaaca taccgtgatt atcgggtaaa aaggccagtg cccagggatg
    21781 gtcgagtttg tcttgcagta cttcgacatt taccgttgca ggagccgccc agagagcgga
    21841 agaaagacaa ataaggggca caaggaaaaa ggattgtcga tgcataacgc ctccggattg
    21901 actctggctt aaagcgtagt cagtggagga gataacaaat tcatttttac aaaaacttaa
    21961 acatgaaggg ggagacgctt tctccccctt agttttcagg ccttctcaag catggcgtgc
    22021 ttctgcaggc tctggatact cagcgttaag ctcatcagac aattttcaag cttatcggcg
    22081 ttgacggtaa taacagtcgg gcaatcatgg tgcccactca tcaaacatac tgcggctgtc
    22141 gctaatgctt cttcagcatg atgaagagca ctccactctt cctgatccag atgaagattc
    22201 aaccgcagcg atttatcgtg cagttcgcga ttcagtttaa aaaagttatc tcgtagatga
    22261 ttgctttcgc tgacggacat gtatcctttt gccttcctca gttgcacgta tgcagcgagg
    22321 tcaacgcgtg cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc aacgaacata
    22381 cttcgatcct cctctttcca gccttgttgc aattaaatag tatggctatt ttttgagcaa
    22441 aatgctgtcc aggatcaatt cttcacgccg aattaacaaa atttacattt ccttcgcctc
    22501 cgccagcgaa tgagattgcg atacaaattt tatccagaac tctgtagaat ccctgccctg
    22561 acctttcctg taactgaata ttttttaagc tatgagcaaa gtaactcccc agccgaaaat
    22621 cggctttgtt tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta ttctcaccga
    22681 actacgcact gaaggttatg acgtggtacc gagctatgac gatgcggaca tggtgatcgt
    22741 caacacctgc ggctttattg acagcgcggt acaagaatca ctggaagcca ttggtgaagc
    22801 gttgaatgaa aacggcaagg taattgtgac cggttgtctg ggggcaaaag aagatcagat
    22861 ccgcgaagtc cacccgaaag tgctggaaat caccgggcct catagctatg agcaggttct
    22921 ggagcacgtt catcactacg tgccaaaacc gaaacacaac ccattcctga gcctggtgcc
    22981 agaacaaggt gtgaaactga cgccgcgtca ttatgcctat ctgaaaattt cggaaggctg
    23041 taatcaccgc tgcaccttct gcattattcc gtctatgcgc ggcgacctgg tgagccgtcc
    23101 gattggcgaa gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta aagagattct
    23161 ggtgatctcg caggatactt ctgcctatgg cgttgatgtt aaacatcgta ctggcttcca
    23221 caacggcgag ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat cgaaactggg
    23281 gatctggaca cgtctgcact acgtttaccc ttatccgcat gtggacgacg tcatcccact
    23341 gatggcagaa ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg ccagcccgcg
    23401 cattctcaaa ctgatgaagc gtccgggttc tgtagatcgc caactggcgc gcatcaaaca
    23461 gtggcgcgaa atctgcccgg aactgaccct acgctcaacc tttattgtcg gcttccctgg
    23521 cgagacagaa gaagatttcc agatgctact cgacttcctg aaagaagcgc gtctggatcg
    23581 cgttggctgc tttaaataca gcccggttga aggtgcagac gccaatgccc tgcctgacca
    23641 ggttccggaa gaagttaaag aagaacgctg gaaccgtttc atgcagctgc agcagcagat
    23701 ttccgccgag cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta tcgacgaagt
    23761 ggacgaagaa ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg acggcgcggt
    23821 ttatctcaat ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga aagtcgagca
    23881 cgccgatgag tacgatttgt ggggtagccg ggtttaatta attcaggccg ggcaaaatca
    23941 tttgtccggc ctgcgaataa tgattactgc cagctaatct gagccctgcc gtttttctta
    24001 ctcttataca gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt acattcattt
    24061 gaatgcgcta caccgatact ggtacttaaa ttaatgcttc ccacctggaa atgaacgatt
    24121 tcttttagaa tcgatagcaa ctcttccagc gttccacccg tataaatcac cgcaaattcg
    24181 tcaccgccga agcggtagat tttaccttta tctaccggga tctgtgattt aaggattttc
    24241 accactttca tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt aatgtttttg
    24301 aatttatcga cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc ttttaaatct
    24361 tcgtcaaagg attttctgcc cattgctttg gtcaggtcgt ccaggaacgc cgagttatac
    24421 atactgtaga gttttttgac atcggcagcc gaaaagaaaa gcccgccatc cagtacgcgt
    24481 tcaaaatggt tattaatgat ggcttctttc tccacaatac cacgattaat cactctgcgt
    24541 accagataga ggcaactggt acccgatatc actgccacca atgcggcaaa gaaaataatc
    24601 aatgtacaga cccgcaaata aacatctttt ttatacgcaa atacgtacaa tccggtcggc
    24661 aaataggcga agtgctccat cgcttccgaa acggtgaaga tggcgttttt agcataatat
    24721 tcatcaccgg tcaccggttt accgttctta aatagagaga acatcaggtt ctcgctgtcc
    24781 atcgccagcg attttgacag taaagaaaaa cgctccagac caagcaaaaa tatcagacta
    24841 cgattgtgag ttaccggata gaggtaatag acgaaggttt taccgtcaaa agagataaac
    24901 cctgtatttt tgacatgtcg atacttcaca acctgcataa tatctgcaca ataatttttc
    24961 tcgaactcct ttacctgaat acattcgtca ctggaaatca gataaggacc gacatcgtac
    25021 aaataagagg gggaaaccga gtcggcgtaa gctgtgcgca aaccaaccaa cagctgttcc
    25081 tccgtgggat ttagcgtgtt gataagatca attgccacgc tacgccgata ttcagcaata
    25141 tttgccaccg caaaagcagt atcaataacg gactgtttca ggctatacac ccgttccttt
    25201 accatttgag tgtagatccc gcaggcaact gctgaaatca tgataaaaat cagcagactg
    25261 actgtaagta cgaacttatt gattctggac atacgtaaac tactctttta ctaattttcc
    25321 acttttatcc caggcggaga atggcatggg tttctggtac agatacccct gagcctgaac
    25381 acatcctaat ttaattaatt cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac
    25441 cgggatccgg ttatagcgtg ccagatcgaa aataatgcgc acaatttttc ccgcagtacc
    25501 gaaaatatcg ccggtaaatt gcgagtccac ctttatgctg tctggattaa tttcataaaa
    25561 acgtttcagg tttgacaaac cggtgccaaa gtcatcaatc gatatccgta ccccttgttt
    25621 acgcagttgt tcaatattga gataaaaatg ttcattaatt cgctcgatca cggtttcagt
    25681 gatctcaaca cagaggctgt tcgccttcag gccattttct ttcatgatgg tggtaaaacg
    25741 ctcatgaaag tgcggttcgt ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc
    25801 atccttcagc gacgcacgct gactaaaatg tctgaattca cccattgccg ttttaataat
    25861 ctgataaccc agctcattga taaacccgat atcttcagca atggtaatga acttcagcgg
    25921 tgaaatgatc ccccgcaccg cagaaaccca acggcacaac gcctccgcgc ccagaatggc
    25981 gcgggtgtta atatcaacga tcggttgata gacgaggaat agttcgccaa tttccattgc
    26041 atggcgaata ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt tcgcgtcatc
    26101 acagaaaata aactcggaga acatatggtc acggatactg gaaatcgcca gtttcagttt
    26161 ttctgcgtaa ctccaggcgt tttccccctg caaaggttca caaatggcag acttatggat
    26221 gcgcagattg cggtagccaa agctgctgtc aaaatcgttt accatcgcta ttttacgggt
    26281 aaatgtctgt acattttccc gacacagaag taaatagaga tcggcattga atgtcacgat
    26341 atcgcagcat tgcgcatatt tctcggcaat tcgctgacac aatttcctga cgattttatt
    26401 gcgttcatca acaccaaaca ccgcttccac ggcttccaga ccacacagtt tgatgatccc
    26461 cacagccaga tactgattat tgttggactc aataatcgcc atatttttaa taaaaccgct
    26521 ttcctgatcg taagagatca gtgagagcaa tttatttgac cgttcaattt cggcatcttt
    26581 catttccgcg atcgaatttt tgatgctgat aatctctttc gcatcgcctt cgcgaatttt
    26641 cagagcctcg atctgatccg ttgagtcggg caaaaacgcc agcgtattaa ccagctcggt
    26701 catttcagaa aattgccgac ggaacacacg agtggtcatg aaatatacca ccagcaacac
    26761 gataataatg aacgcaaacg gtagataaaa aagtttatct gatgcgttca ataaattacc
    26821 gccaggataa taaaaataga tttcgaacaa tgcattattt ggcactgctg atgtctttgc
    26881 aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat tataaataat
    26941 cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt tcaaaaatag
    27001 aacttcatca ttagcgctga tttgatcaag atactcgttc agataccgcc agtttaaatc
    27061 gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac gtagcgtaaa
    27121 agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc agctgttgtt
    27181 agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg caaaaataac
    27241 ccgccctgac tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa caacgatttt
    27301 atcattcgcc ctccgtgaaa agagataata tttattatca tcaagtacca gtcccaccga
    27361 gtctaaaaat ggcatcattt ccattttcag aatcacggcg cgttgaatct cctctgtatt
    27421 ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat ctttaaagaa
    27481 atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc
    27541 caataccagt tgatagttaa tgatgaaaaa aagaccaata aatgacagtg ctgccaataa
    27601 aaataaaatt atcagcctta tctttatctt ttcgtataaa ctcagcatgt taatacattt
    27661 aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt
    27721 aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct tagggcaata
    27781 atttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc ctttaatttt
    27841 cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta cggtcagaaa
    27901 aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg ctcgtgcctc
    27961 attgagcatt gctccccact ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag
    28021 gctggcggca gagataatcg aggtgccaat gcgcatggtg aaaaacacca cgatagacga
    28081 gacggtccca ggcaagatat gacgcaataa aatggtcata tcgctggcac caatactgcg
    28141 tgctgactca ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca ccagtcgggc
    28201 aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc cgcttcccaa
    28261 caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacagca catcgcaaat
    28321 gcgcatgatc aaccgatccc accagccttc ataatatcca gccaacaagc ccagcaacgt
    28381 cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc
    28441 aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa
    28501 agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat agggagcgat
    28561 ccagcgtgca aaaatggcca ccacaatcaa taaaataacg aataatgcgg cggtcatcgc
    28621 catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca cctggtcagg
    28681 tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct
    28741 tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac taagttgata
    28801 agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt
    28861 tctacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg
    28921 atagaaccac cgagcaaaaa gccaaactgt aagcccatca tggtcactac cgggatcatc
    28981 gcgttacgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt cgccctcgcg
    29041 gtacgcatat aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa gcgcgccatc
    29101 acggctgcca ctgccgcgcc gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct
    29161 gctcccacgg taggcagcca gcccagttca acggagaata cctgaattaa aagcatcccc
    29221 agcgcaaatg ccggaaacga gatccccgac accgcaatgg tcatactcaa tcgatccggc
    29281 caacgattac gccagacggc ggcgataatt cccgccgcca taccaaatat caccgcccag
    29341 accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat ctcatcggca
    29401 accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt gctgatatag
    29461 tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac cagttctata
    29521 acctgcgcat cagcttcggg cccggcaatc aatcgcgccg gatcgccggg cagcatatgc
    29581 acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc cagtaagcgt
    29641 ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa
    29701 agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg tgtgccgaca
    29761 ccagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata tcctgcgccg
    29821 ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga gccaggaagt
    29881 cgtccacctg tttattgctg taaaatgcgg tattaaacag cgtcggtggc cagttttgcg
    29941 aggcgaacag tggcgatagc gcccagtccg cttcgccggt tgaagccgac cagccagtgt
    30001 agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc gcccgctgtc
    30061 cggcatccat cgcggtcacc tgggctttaa tcccgacctg cgctaactgc tgctgggtaa
    30121 attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac
    30181 cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg taaggccacg
    30241 gtttataact ttgcgcgtag gcgatacttg gcggtaccac accagtagct ggcgttgcat
    30301 agcccgcaaa ggcaactttc accagcgccg gacggttaat ggcgtaattc agcgcctcac
    30361 ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa cgctgcataa
    30421 ttgacggact ggccatcaac tcgatatttt tgtttttctc cagcagtgtg gcctgctcgt
    30481 agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg cgggtgttgt
    30541 tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc tgccagtaac
    30601 ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt tcatacggtc
    30661 cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct gccggtgaaa
    30721 tcatcgcggt cgccggatgg gcaagaatat taataaacgc tgagaacggc tgtttgaggg
    30781 taatctttac cgttgtcgga tcgatcgcct ccgttttggc aatattctta tacaggttat
    30841 agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttt accgccactg
    30901 cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg caatttcacg atgtaagtaa
    30961 tgccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc atctctttat
    31021 ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac gtgtcatttg
    31081 cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc caccaccaca tctttggcag
    31141 cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac ccactacggt
    31201 gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa tgcgtattct
    31261 gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac ggctgccacc
    31321 tcttcgccac gcagatgaat attgctggga agatcgtccg acagcagtac acgctgcggt
    31381 cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt ataaggatgc
    31441 tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc gagatacatc
    31501 accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat aaacagatac
    31561 gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc gcgaatagaa
    31621 acatccagcg ccgaaacggc ttcgtcggca atgatcactt ttggattcaa tgccaacgcg
    31681 cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgcgggta acgccaggca
    31741 tgttcaggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc cgcgtcttta
    31801 cctggcaata aaccgtgtac acgcagcggt tcgataatcg aatcaccgat ggtctgacgt
    31861 gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg caatgcctga
    31921 agtttgccgg gtgacaaggt atcgattctc tgaccgttaa agataatttc gccgccctgc
    31981 gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc agactcgccc
    32041 accagcgata gcgtttcgcc aggccagaga tcaaaactaa ctttctcaac ggcatgcact
    32101 tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac aagattacgc
    32161 actcgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg ggcctgtttc
    32221 gctggatgtt caagcgatat caacgggaaa cgtcggggat aatctaaccc tttcatcgca
    32281 ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa
    32341 atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg
    32401 gcaatctctg ccaccacgcc catatcgtga gtgataaaga taacgcccat cgacatctct
    32461 ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg
    32521 gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact
    32581 cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt ttgtgcctca
    32641 ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg
    32701 ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac cgggttcagc
    32761 gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg gcgcatttgt
    32821 gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa cagcatttta
    32881 tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa cgcagtcact
    32941 gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg cagactaaaa
    33001 gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat attcagattt
    33061 tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt
    33121 ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc
    33181 ttcggtgtta aacggtagcg cgacattccc ttcatggtcg atagcgatta agccaccgct
    33241 accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa
    33301 tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc
    33361 gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac
    33421 taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc
    33481 tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt
    33541 ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc gcgctgccag
    33601 tagttgttca taacgcaaag gcgtggagaa aatctccggc gagacgcgct ccatgccacg
    33661 agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat
    33721 caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc
    33781 tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa
    33841 gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt
    33901 caccacatcc agcgcacttt cgcccgcttc caacattttc tgcccggttt caacaatggc
    33961 agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc
    34021 acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagggtg
    34081 gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat
    34141 atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac
    34201 agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt
    34261 tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc
    34321 gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt ggtcgtattc
    34381 tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc gcaatggacg
    34441 gctacgcggt gcgtttagcc gatattgcct ccaggcaacc gctgcccgtt gccggtaaat
    34501 cctttgccgg tcagccgtat catggtgaat ggcctgcggg cacctgcatt cgtattatga
    34561 ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa
    34621 cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg
    34681 gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgcag
    34741 agctgccagt aattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg
    34801 tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg ggcgacggcc
    34861 aaatctacga caccaaccgt ctcgccgtac acctgatgtt agaacagctg ggttgcgagg
    34921 taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca tttattgaag
    34981 ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt
    35041 acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac
    35101 caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca
    35161 acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa
    35221 gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga
    35281 aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg
    35341 aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg
    35401 gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggaagtgggc gaatgggtgg
    35461 aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg acggaactca gcgatcagga
    35521 gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc
    35581 gctaaaagat tctcgtgtgc tggtagtcgg cctcggtgga ctcggctgcg cagcttcgca
    35641 gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgact cggtttcgct
    35701 ctcgaatctg caacgccaga cgctgcacag tgatgctacg gtcgggcaac cgaaggtaga
    35761 atccgcccgt gacgccatga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc
    35821 actgcttgat gacgctgaac ttgctgcgat gattgcaaaa cacgatctgg tactcgactg
    35881 tacggataac gttgcggtgc gtaatcaact gaacgctggc tgttttgccg cgaaggtgcc
    35941 gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga
    36001 cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcat taacctgcgt
    36061 agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg caatggaagc
    36121 gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc
    36181 aatgacctgt cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg
    36241 gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc atcagcaatt
    36301 tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc agccagtcct
    36361 gttcaaactt cgccacagcg gcttccaccg ccggatagct aatcatctgt tgtgccacat
    36421 ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc tgacgcgggg
    36481 ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta
    36541 agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg
    36601 caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca tataccgcgg
    36661 ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccatc ccctcggcgg
    36721 tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt gcgtcattca
    36781 ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg
    36841 cttcatgaag ttgcggaagc acaacatcca gcggtttgtt acccgcggcg ataatgcttg
    36901 ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcacc gcaacaacgt
    36961 ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta
    37021 gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatctaactt tcattcgaaa
    37081 gcattttaat gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact
    37141 cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct
    37201 gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg tatgacgcac
    37261 gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc gccggaagtg attgagcgcg
    37321 cgctgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg acggcgttaa
    37381 ccgactgctg tccgacacag gcattaaccg tgtgtggtga agtgaaaagc gttgaggaga
    37441 tcatgacgac cgttctgcgc gataaaccgt tttatgatcg cagcggcggc ggtttaacgc
    37501 tttcgggtgg tgagcccttt atgcagccgg aaatggcgat ggcgctactg caagccagcc
    37561 acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatcg
    37621 ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacgtt gccgacgcgc
    37681 cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct ggataacctg aaaaaactcg
    37741 ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg
    37801 aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc
    37861 attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg
    37921 acgccccgga aaaaccgctt gatgcgccag aactgctcga ctttgcccag cagtatgcct
    37981 gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga
    38041 cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc
    38101 agtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc
    38161 gatcccggta cgtcgcgcgc tggcactggc gcatcacctg gcgaatcgca ccatctggat
    38221 caaacacgat gagttgatca ttggcaacca ggcaagcgaa gttcgcgccg cgccgatctt
    38281 cccggaatat actgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg
    38341 tgctggcttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt gcccgtggtg
    38401 gcgcggtcag accgtacagg atcgctgcta cggcatgttt accgatgagc aaaaaggtct
    38461 gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc
    38521 ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgtgaga aagtagcgga
    38581 acgtcgctcg cgcatcaatc tgacggtgct ggaagattta catggcgaac aattcctgaa
    38641 agcgattgat atcgtgctgg tggcagtcag tgaacacatt gaacgtttcg ctgccctggc
    38701 gcgtgaaatg gctgctaccg aaactcgcga aagccgtcgc gatgaactgc tgacgatggc
    38761 agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact
    38821 gtgttacttc atccaattga ttttgcagat cgaatctaac ggtcactcgg tatcgtttgg
    38881 tcgtatggac cagtatctct acccgtacta tcgccgcgac gttgaactca accagacgct
    38941 ggatcgcgaa cacgccatcg agatgctgca tagctgctgg ctgaaactgc tggaagtgaa
    39001 caagatccgc tccggctcac actcaaaagc ctctgcggga agtccgctgt atcagaacgt
    39061 taccattggc ggacaaaatc tggttgatgg tcaaccaatg gatgcagtga atcccctctc
    39121 ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg
    39181 ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga tccgttgcgg
    39241 cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta ttaaactcgg
    39301 tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa ccgccgtcgg
    39361 tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct
    39421 ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa gtgttcctgc cacaagaaaa
    39481 agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt gggatacgca
    39541 aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga
    39601 agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa
    39661 aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg ttggcattgc
    39721 caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg gcgcgattgg
    39781 tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact
    39841 gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ctgtcgatac
    39901 gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc ataatccgcg
    39961 ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta tctccgctaa
    40021 cgtaccgttt ggcgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct
    40081 ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg
    40141 ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact
    40201 gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc tgctgcgcac
    40261 cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct
    40321 gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg tcgcgggtta
    40381 ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg ctcgtactga
    40441 acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca
    40501 tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta
    40561 cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca
    40621 cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg
    40681 cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc ggtaatcagt
    40741 attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa
    40801 acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg
    40861 aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg
    40921 gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa
    40981 aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac gtactgttca
    41041 agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa ctgcacatag
    41101 cgctcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc
    41161 ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc
    41221 aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt
    41281 attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg
    41341 atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat aacacatccc
    41401 cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt ctttttacat
    41461 cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt atattgtcac
    41521 atgcaaatca gaatggtttc gaatgaaagt taatgagagg tggttatggc ttctactttt
    41581 accagcgaca cattgcctgc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg
    41641 cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga cattgccacg
    41701 cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct
    41761 tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc
    41821 ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg
    41881 atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat
    41941 ttcttcggga cgctcagcgc ttcacgtccc gagatttacc ccatctactg gtcgcaggcg
    42001 caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg
    42061 tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct
    42121 gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc gcataccgac
    42181 tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc caacgtcttt
    42241 aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga agttgaagag
    42301 tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggacagcg
    42361 ctctctgata tgctgcctgg tcaggggctg ctgcacgttg tgcccattcc tgaagccatg
    42421 gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta
    42481 gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta
    42541 accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat
    42601 tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca
    42661 ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc
    42721 gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa actgggaagg acgctttacg
    42781 cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg
    42841 gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt
    42901 gcgctttctt aatgcctgat gcgctacgct tatctggcct acaaagtcct gcagattcaa
    42961 tatattacag gtac
//
LOCUS       DAZFKM010000026        41407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFKM010000026 DAZFKM010000000
VERSION     DAZFKM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHKAY_001544"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHKAY_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO5700243.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="ACHKAY_001545"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="ACHKAY_001545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO5700244.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="ACHKAY_001546"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="ACHKAY_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO5700245.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHKAY_001547"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHKAY_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO5700246.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQPPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="ACHKAY_001548"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="ACHKAY_001548"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO5700247.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="ACHKAY_001549"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="ACHKAY_001549"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO5700248.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="ACHKAY_001550"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="ACHKAY_001550"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO5700249.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="ACHKAY_001551"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="ACHKAY_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO5700250.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHKAY_001552"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHKAY_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO5700251.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9447..10292
                     /gene="glpF"
                     /locus_tag="ACHKAY_001553"
     CDS             9447..10292
                     /gene="glpF"
                     /locus_tag="ACHKAY_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO5700252.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10315..11823
                     /gene="glpK"
                     /locus_tag="ACHKAY_001554"
     CDS             10315..11823
                     /gene="glpK"
                     /locus_tag="ACHKAY_001554"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO5700253.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11958..12968
                     /gene="glpX"
                     /locus_tag="ACHKAY_001555"
                     /pseudo
     CDS             11958..12968
                     /gene="glpX"
                     /locus_tag="ACHKAY_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            13065..13811
                     /gene="fpr"
                     /locus_tag="ACHKAY_001556"
     CDS             13065..13811
                     /gene="fpr"
                     /locus_tag="ACHKAY_001556"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO5700254.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13816..14244)
                     /gene="uspD"
                     /locus_tag="ACHKAY_001557"
     CDS             complement(13816..14244)
                     /gene="uspD"
                     /locus_tag="ACHKAY_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO5700255.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14271..14570)
                     /gene="yiiS"
                     /locus_tag="ACHKAY_001558"
     CDS             complement(14271..14570)
                     /gene="yiiS"
                     /locus_tag="ACHKAY_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO5700256.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14782..15222)
                     /gene="yiiR"
                     /locus_tag="ACHKAY_001559"
     CDS             complement(14782..15222)
                     /gene="yiiR"
                     /locus_tag="ACHKAY_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5700257.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15323..15922
                     /gene="yiiQ"
                     /locus_tag="ACHKAY_001560"
     CDS             15323..15922
                     /gene="yiiQ"
                     /locus_tag="ACHKAY_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO5700258.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16030..16797
                     /gene="tpiA"
                     /locus_tag="ACHKAY_001561"
     CDS             16030..16797
                     /gene="tpiA"
                     /locus_tag="ACHKAY_001561"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO5700259.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16852..17607)
                     /gene="cdh"
                     /locus_tag="ACHKAY_001562"
     CDS             complement(16852..17607)
                     /gene="cdh"
                     /locus_tag="ACHKAY_001562"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO5700260.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17714..18703)
                     /gene="sbp"
                     /locus_tag="ACHKAY_001563"
     CDS             complement(17714..18703)
                     /gene="sbp"
                     /locus_tag="ACHKAY_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO5700261.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19022..19984)
                     /gene="pfkA"
                     /locus_tag="ACHKAY_001564"
     CDS             complement(19022..19984)
                     /gene="pfkA"
                     /locus_tag="ACHKAY_001564"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO5700262.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20165..21067)
                     /gene="fieF"
                     /locus_tag="ACHKAY_001565"
     CDS             complement(20165..21067)
                     /gene="fieF"
                     /locus_tag="ACHKAY_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO5700263.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21216..21716)
                     /gene="cpxP"
                     /locus_tag="ACHKAY_001566"
     CDS             complement(21216..21716)
                     /gene="cpxP"
                     /locus_tag="ACHKAY_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO5700264.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21866..22564
                     /gene="cpxR"
                     /locus_tag="ACHKAY_001567"
     CDS             21866..22564
                     /gene="cpxR"
                     /locus_tag="ACHKAY_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO5700265.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22561..23934
                     /gene="cpxA"
                     /locus_tag="ACHKAY_001568"
     CDS             22561..23934
                     /gene="cpxA"
                     /locus_tag="ACHKAY_001568"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO5700266.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24076..24750)
                     /gene="yiiM"
                     /locus_tag="ACHKAY_001569"
     CDS             complement(24076..24750)
                     /gene="yiiM"
                     /locus_tag="ACHKAY_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO5700267.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24899..25882)
                     /gene="kdgT"
                     /locus_tag="ACHKAY_001570"
     CDS             complement(24899..25882)
                     /gene="kdgT"
                     /locus_tag="ACHKAY_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO5700268.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26142..26762)
                     /gene="sodA"
                     /locus_tag="ACHKAY_001571"
     CDS             complement(26142..26762)
                     /gene="sodA"
                     /locus_tag="ACHKAY_001571"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO5700269.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27047..28081
                     /gene="rhaT"
                     /locus_tag="ACHKAY_001572"
     CDS             27047..28081
                     /gene="rhaT"
                     /locus_tag="ACHKAY_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO5700270.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28078..28926)
                     /gene="rhaR"
                     /locus_tag="ACHKAY_001573"
     CDS             complement(28078..28926)
                     /gene="rhaR"
                     /locus_tag="ACHKAY_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO5700271.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29000..29837)
                     /gene="rhaS"
                     /locus_tag="ACHKAY_001574"
                     /pseudo
     CDS             complement(29000..29837)
                     /gene="rhaS"
                     /locus_tag="ACHKAY_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            30125..31594
                     /gene="rhaB"
                     /locus_tag="ACHKAY_001575"
     CDS             30125..31594
                     /gene="rhaB"
                     /locus_tag="ACHKAY_001575"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO5700272.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31591..32850
                     /gene="rhaA"
                     /locus_tag="ACHKAY_001576"
     CDS             31591..32850
                     /gene="rhaA"
                     /locus_tag="ACHKAY_001576"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO5700273.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32933..33757
                     /gene="rhaD"
                     /locus_tag="ACHKAY_001577"
     CDS             32933..33757
                     /gene="rhaD"
                     /locus_tag="ACHKAY_001577"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO5700274.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33829..34977
                     /gene="fucO"
                     /locus_tag="ACHKAY_001578"
     CDS             33829..34977
                     /gene="fucO"
                     /locus_tag="ACHKAY_001578"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO5700275.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            34974..35288
                     /gene="rhaM"
                     /locus_tag="ACHKAY_001579"
     CDS             34974..35288
                     /gene="rhaM"
                     /locus_tag="ACHKAY_001579"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO5700276.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35601..36047
                     /gene="frvA"
                     /locus_tag="ACHKAY_001580"
     CDS             35601..36047
                     /gene="frvA"
                     /locus_tag="ACHKAY_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO5700277.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36058..37509
                     /gene="frvB"
                     /locus_tag="ACHKAY_001581"
     CDS             36058..37509
                     /gene="frvB"
                     /locus_tag="ACHKAY_001581"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO5700278.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37499..38569
                     /gene="frvX"
                     /locus_tag="ACHKAY_001582"
     CDS             37499..38569
                     /gene="frvX"
                     /locus_tag="ACHKAY_001582"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO5700279.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38569..40317
                     /gene="frvR"
                     /locus_tag="ACHKAY_001583"
     CDS             38569..40317
                     /gene="frvR"
                     /locus_tag="ACHKAY_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO5700280.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISDSGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40367..41407)
                     /gene="yiiG"
                     /locus_tag="ACHKAY_001584"
     CDS             complement(40367..41407)
                     /gene="yiiG"
                     /locus_tag="ACHKAY_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO5700281.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT        10060 a        10707 c        10711 g         9929 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tggacaatgc tactgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgtagccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagct gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 cagcaggagc gcatcgggat tgtggtatca gttagcgatg ccagcgaact gccgctcaat
      901 gagctaaaag cggtagtcga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggtca ggcggtggat cttaacagcc tgaaacgttc gccaaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgcggaactt
     1201 gaatttaatg atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaagccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttcggcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc
     1861 cgtcgggcag ggaatgcgcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac
     2041 tgcggctgga tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgatgt tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcgctgg aacagcaact ggcggaagtg catcgcggcg gcgcgaggat cttgattggc
     2341 acgcaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac
     2461 acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg
     2521 caccatccgg aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatccttt ccagcccgct ggcagacgac aaactgtggg ttctcggccc ggttccggct
     2761 ctggcaccta agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc
     2881 cgcaaggtga aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtgccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aattcgcacc ggaactggag ctacctacag ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatctatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc gctgtgtcac taccgcctgc aaggttacgt tcaggcgctg cgtcgctgcg
     3721 gcattatggt tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcgatgca gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggtaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct
     4801 tctactcagc agccgtacca ggatctgctg caaccccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gagacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggtcaa agccgccgtt gagctagcga aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgtgcactg ttagaaaata ctgaactgag tgctcgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtaggct
     6061 acgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa gcgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcacttc gatccgctgg
     7321 tcgccctgct ggcactaatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggttg gcactggttg cagtggcatg ccatacgctg gccgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt
     7681 tgagtgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata
     7801 tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctcgctggt gtgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac
     8041 gtactgtcaa gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttctga agatccgctg gatattgaat gatagaaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 ggaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc
     9061 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
     9121 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
     9181 tttcatcctt ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc
     9241 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
     9301 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgcttt tgctcgttaa
     9361 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
     9421 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
     9481 ttgctgaatt cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac
     9541 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
     9601 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
     9661 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
     9721 cacaagttgc cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat
     9781 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
     9841 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
     9901 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggat gatggcaacg
     9961 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    10021 catctatggg cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
    10081 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    10141 acttcctggt gccgcttttc ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc
    10201 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca
    10261 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    10321 gaaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    10381 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    10441 ccaggttggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    10501 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    10561 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    10621 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    10681 gattatatcc gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg
    10741 aagtggatcc tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg
    10801 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    10861 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    10921 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    10981 gaagtatacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    11041 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    11101 aagaacacct atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa
    11161 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    11221 ttggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    11281 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    11341 gtgtatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    11401 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    11461 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    11521 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    11581 cagtccgata ttctcggcac ccgcgttgag cgcccggaag tgcgcgaagt caccgcattg
    11641 ggtgcggcct atctcgcagg cctggcggtt ggcttctggc agaacctcga cgagctgcaa
    11701 gagaaagcgg tgattgagcg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    11761 cgttacgcag gctggaaaaa agcggttaaa cgcgcgatgg cgtgggaaga acacgacgaa
    11821 taatgtaaat gccgaatgaa gcgtttatgc cgcatccggt agtcccgaaa cgtgcggggg
    11881 caaccccgca cacatcaata atccctccct tcccctgtgc tacacttcgc gccattcctt
    11941 actgcttaga gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat
    12001 cagcggcgct ggctggctac aaatggttag gacgcggcga taaaaacacc gcggacggcg
    12061 cggcggtaaa cgccatgcgt attatgctca accaggtcaa cattgacggc accatcgtca
    12121 ttggtgaagg tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg
    12181 gtcgcggcga cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga
    12241 tgggccaggc taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaatg
    12301 cgccggatat gtatatggag aagctgattg tcgggccggg agccaaaggc accattgatc
    12361 tgaacctgcc gctggcggat aacctgcgca atgtagcggc ggcgctcggc aaaccgttga
    12421 gcgaactgac ggtaacgatt ctggctaaac cacgccacga tgccgttatc gctgaaatgc
    12481 agcaactcgg cgtacgcgta tttgctattc cggatggcga cgttgcggcc tcaattctca
    12541 cctgtatgcc agacagcgaa gttgacgtgc tgtacggtat tggtggcgcg ccggaaggcg
    12601 tagtttctgc ggcggtgatc cgcgcattag atggcgacat gaacggtcgt ctgctggcgc
    12661 gtcatgacgt caaaggcgac aactaagaga atcgtcgcat tggcgagcag gagctggcac
    12721 gctgcaaagc gatgggcatc gaagccggta aagtattgcg cctgggcgat atggcgcgca
    12781 gcgataacgt catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggcatta
    12841 gccgcaaagg caatatcgcg actaccgaaa cgctgctgat ccgcggcaag tcacgcacca
    12901 ttcgccgcat tcagtccatc cactatctgg atcgcaaaga cccggaaatg caggtgcaca
    12961 tcctctgatt gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt
    13021 cggagaacga agataaggca agtcaatcaa aacaggagaa aaacatggct gattgggtaa
    13081 caggcaaagt cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg
    13141 cccccgtgct tccgtttacc gccgggcaat tcaccaagct tggccttgaa atcgacggcg
    13201 aacgcgtcca gcgcgcctac tcctatgtta actcgcccga taatcccgat ctggagtttt
    13261 acctggtcac cgtccccgat ggcaaattaa gcccacgatt ggcggcactg aaaccaggcg
    13321 atgaagtgca ggtggttagc gaagcagctg gcttctttgt tctggatgaa gtaccggact
    13381 gcgaaacgct atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc
    13441 aactaggcaa agatttagat cgcttcaaaa atctggtcct ggttcacgcc gcacgttatg
    13501 ccgccgactt aagctatttg ccactgatgc aagaactgga aaaacgctac gaagggaaac
    13561 tgcgcattca gacggtggtc agtcgggaaa cggcagcggg gtcgctcacc gggcggatac
    13621 cggcgttaat tgaaagtggg gaactggaaa gcgcgattgg cctgccgatg aataaagaaa
    13681 ccagccatgt gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga
    13741 aagagacccg gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc
    13801 attactggta agcggttact tatcgataaa cggcacgatg agcaaatccg cactcatctt
    13861 attgatcatc ccgcgatatg ccggcatcaa acggttgata aatgagtgat gatgaccaca
    13921 gacaaggagg tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc
    13981 gcgttcaata cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt
    14041 gttatccgac ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc
    14101 cggatacaac tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg
    14161 tctggcgagc tccagagctt tattcaccag taaggcatct tcttcattcc cggaaattgc
    14221 cacgccaatg tgtttataag ccatgtttaa ctccttctaa agcaactcca tcaagctaac
    14281 gaacgcagtg ataactcaaa aataatcatc tcggcctggc atgagaaagt gaaggccgca
    14341 tcaagctcaa cttgcccctc cagatcctta atcttgtagg ccacgctggc cccttcatct
    14401 gcagaggatg cagcggcggc aagctctttc agtttggtca taaaagactc tgcttcttcg
    14461 cgggtggcaa aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg
    14521 atatcaatcg cacatccttt agtactgcat ttatctacga catctttcat agggcacctc
    14581 tgtatattcg cctctctgtc gattacgact taagaataat gcgccgttaa gggaagaaca
    14641 aacaagtaat gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca
    14701 gaaggatttt ccgggtattg tcatttgcat cgtggaaata acaatcccgg aaaaaaatag
    14761 cagtaagaga tatattaaag gttaattact tgatttattg tcggctttat acttcacatc
    14821 ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat
    14881 aagcatcatc accagaatca acgtcgggac aaaacgcccc accgcccatt gccagacacc
    14941 cggtaaaatc gcccagttac ccgccagcag catccacgcc acaatcatca gaaatgccca
    15001 tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt actgccgctg ttggccaaag
    15061 caagcacaca aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga
    15121 gaaaagcacc agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc
    15181 aatacgccct ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt
    15241 gtcttttcca ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca
    15301 gtcataacaa aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc
    15361 tgcattaacc gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc
    15421 ttatctgctg gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt
    15481 taacagccag aatcctagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga
    15541 caaagccaat ctcactcgtg ctgcaagtaa aattaacgag aacttgtatg cttctacagc
    15601 gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg acctggctac ccatccaggg
    15661 gccagagcaa aaagcagcga aagcgaaagc tcaggaatac atggcagcag tgatccgcac
    15721 actcacccca ttaatgacca aaacacaaag ccagaaaaaa ctgcagtcgc tactaacggc
    15781 ggggaaaaac aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga
    15841 caacggcgaa aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc
    15901 gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact
    15961 gtttcacaga cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg
    16021 agaattaaaa tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac
    16081 atggttcacg agctggtttc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg
    16141 gttgcaatcg caccaccgga aatgtacatc gatatggcga agcgcgaagc tgaaggcagc
    16201 cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa
    16261 acttctgccg ctatgctgaa agacatcggc gctcagtaca tcatcatcgg ccactctgaa
    16321 cgtcgtactt accacaaaga gtctgacgaa ctgattgcga aaaaattcgc ggtgctgaaa
    16381 gagcagggcc tgactccggt tctgtgcatc ggtgaaaccg aagctgaaaa cgaagcgggc
    16441 aaaactgaag aagtttgcgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg
    16501 gcattcgaag gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct
    16561 gcaactccgg ctcaggcaca ggctgttcac aaattcatcc gtgaccacat cgctaaagtt
    16621 gacgctaaca tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac
    16681 gctgcagaac tgtttgctca gccagatatc gacggcgcgc tggttggcgg tgcttctctg
    16741 aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc
    16801 tgacaggtgc cggatttcat atccggcact tactttcctt aactcctcgc cttaacgcaa
    16861 aatctcacac tcatgatcct ggatttcctc ggctgaagca cggttcagcg tcagtagatt
    16921 tcgttgtgtc gccagcaaca caaacgaatt atcactctgc cgtaccatcg ccagcccgta
    16981 gcttcccata tgttcccgcg cctcaggcac ttcttctgca agcatcataa atgggctgcg
    17041 ttgtaccagt tcgctttccg ttacccgacg cgccaggtat tcatgcccgc gcaaaccacc
    17101 tggcagtggc aaccagcggc tgctgatgtt cgccagattt ttatccagtt gttcgcgcac
    17161 atcaggacga atacaagaga tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga
    17221 gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg ccgtattttt tgctcataaa
    17281 atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt gaaggatcgg tcaacaaagg
    17341 actttcagta ccgttaatac gatacgttgg catcaacaga tattgcagtg gaccattaag
    17401 atcttttaaa accacgtatc cggcattggg tttgacttcc gcacatggcg aaggattttg
    17461 attttgctgc tgattgggca aacattcctc aaggacaatc ttacgtaatg tatccgactc
    17521 ttcaccggtt aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac
    17581 caaaaaaaga agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg
    17641 gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag aaaccaccgg gtttgataat
    17701 tatcctgggg aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct
    17761 ctttttgcgc tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcagctttg
    17821 gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg cggacggtag tagtttttcg
    17881 cggcaatttc ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt
    17941 tggtgccttt tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag
    18001 actcactcgg cgtgacgatt tcgaatttat ctttccccag ttcattcgct accagcagag
    18061 cttcgttttc ccaggcgatc agtacatcgc caatcccgcg ctcgacaaag gtgttagtgg
    18121 agccgcgcgc gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct
    18181 gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta gccccaggca gccaggtagt
    18241 tccagcgtgc gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa
    18301 tcagatcgtt ccagtcatgg atctgcttcg gattgccctt acgcaccagg aaaacaatgg
    18361 tggaagtgta cggtgcggag ttatccggca gacgtttgat ccactcttta tcaatccgcc
    18421 cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa
    18481 taccgttgat taccgacgtc gcttgtttac ctgaaccacc gtgtgactga cgaatcacca
    18541 cgttatcacc agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca
    18601 attcgcgcgt tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgttgg
    18661 ttgccgccag caaaaatgtt aaccctacgc cccacttgtt catcgcccga ctctcttatg
    18721 ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa acattaaaga ataaaaaaag
    18781 attggctata acttgcgggt atatgttggg ggttaaaaag gcggaagaaa tccgcctcat
    18841 attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg cgaacgcctt atccggccta
    18901 caaaagtttg caaattcaat aaattgcaga attcatgtag gcctgataag cgaagcgcat
    18961 caggcatttt tgcttctgtc atcggtttca gggtaaagga atctgccttt ttccgaaatc
    19021 attaatacag ttttttcgcg cagtccagcc agtcaccttt gaacggacgc ttcatgtttt
    19081 cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt ctggatacct acacaacgac
    19141 cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc catacgggaa gccagaatac
    19201 ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc cagcacagtt gcgcgggttt
    19261 cacgaccggt ttctttctcg atgaaatgcg ccagttcgtc aacatcacac atatgttcgg
    19321 taatcgccac gatcgcgtgt tttttacctt tcgcgatacc cgctttgatt tcgtttacca
    19381 ggtcttcacg gctgaattca acttccggaa ccacaacgaa ttcacagcca ccggcaatgg
    19441 ccgcagccaa cgtcagatcg ccacaataac ggcccatcac ttccaccacg gaaatacgct
    19501 ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc tacaacggtg ctcagcgcag
    19561 tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt gtcgatagtg cccggcagac
    19621 cgatgcacgg gaagcccatt tcggtcagac gcattgcacc catgtaggaa ccgtcaccgc
    19681 cgataaccac cagcgcgtcg atcccacgtt ttttcaggtt ttcgatagcc acggcgcgga
    19741 tgttctcgtc gcggaattcc gggaagcgcg cagaaccgag gaacgtaccg ccacggttga
    19801 tcatgtcaga aacgctgtaa cggtctagct gtaccatacg gtcttcatac agacccagat
    19861 agccgtcata aatgcccatt acttccagac cttctgtcag cgcagaacga acaaccccgc
    19921 gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt caacacaccg attttcttaa
    19981 tcatgactac ctctgaactt tggaatgcaa aataaaatct gttgccggaa gtcttcttgc
    20041 acatcgaagt gatccaacga atgtgcaaat agtataacaa tcacttcctg ctgaattgat
    20101 tcaggtcagg ccaaatggcg gtattttata cacaaaatgc gggtctggct ctcttttata
    20161 ctgattatga aagcatagac cgtttaccct ccctgggtac gacggaacag gggtcctgat
    20221 ggataattac atccgatccc ggaaaacgcc gtaaaatagc ctgctctacc tgatccgcca
    20281 ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc caaatgaatc tgaataaagc
    20341 gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc gctaacaccc ggccaggaag
    20401 tcacgatatc aataatttct tgccgttcct catcaggcaa tgcgcgatcc agtaatgact
    20461 gtaccgcctc atatcccatg cgtaacgcgc tatataaaat atagatgccg attcccaatg
    20521 caaacagagc atcggcgcga tgccagccgt accaggacaa ccccagcgcc agcagaattg
    20581 cgccgttcat cataacatca gactggtaat gtagcatatc agcccgcacc gcctggcttt
    20641 gcgtccggcg caccacccaa cgctgaaacg agacaaggat aatcgtacaa attagcgcca
    20701 caattgtcac gataaccccg acgcctggat ctgtcatcgg tgttggagat atcagatgtt
    20761 gaatacccgt caaaaacagg aatagtgccg aaccggagat aaacatactt tgcgccagcg
    20821 ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt atcgtcggca ggttgcaggg
    20881 aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat atccaccagc gaatccacca
    20941 gcgcggcgag aatactcacc gacccggtat accaccatgc aaaaatttta atcagcaata
    21001 gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact gaccagccgt ccataagatt
    21061 gattcataaa tactcccgct atcaactgac gctagtataa cggaagcaaa tcatctgcaa
    21121 tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc cacatcatgt gggggaagac
    21181 agggatggtg tctatggcaa ggaaaacagg gtttactact gggaacgtga gttgctacta
    21241 ctcaatagct tcaacgatga acttttttgc cattgcgtca cgtcacgcaa ctgctccatt
    21301 cgttgttgat gtttctcgtt taaaaccgct tgctgctccg gcgttaacag gcgatacatt
    21361 tggttgcgga cttttgccat ctcaacctga cgagcaattt gctcattcgc cattttttct
    21421 gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg tgacaaggcg atgcattgtc
    21481 tccagttcgc taacattaac aggaggctgt tcgtgccggg cctgttgcat aagatctcgc
    21541 atctgctgac gctgatgttc ggttaaactt atgccgtcga acatatggct ctgcgtactg
    21601 cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg aaccgacttc agcagcgtgg
    21661 cttaatgaac tgactgccag cgttgaggcc atgacggcag cggtaactat gcgcatcatt
    21721 tgctcccaaa atctttctgt cgcgattcaa cgatagagag tttacgattc aggctgcaaa
    21781 catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt agcgacgtct gatgacgtaa
    21841 tttctgcctc ggaggtattt aaacaatgaa taaaatcctg ttagttgatg atgaccgaga
    21901 gctgacttcc ctattaaagg agctgctcga gatggaaggc ttcaacgtga ttgttgccca
    21961 cgatggggaa caggcgcttg atcttctgga cgacagcatt gatttacttt tgcttgacgt
    22021 aatgatgccg aagaaaaatg gtatcgacac attaaaagca cttcgccaga cacaccagac
    22081 gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat cgcgttctcg gccttgagct
    22141 gggcgcagat gactatctcc cgaaaccgtt taatgatcgt gagctggtgg cacgtattcg
    22201 cgcgatcctg cgccgttcgc actggagcga gcaacagcaa aacaacgaca acggttcacc
    22261 gacactggaa gttgatgcct tagtgctgaa tccaggccgt caggaagcca gcttcgacgg
    22321 gcaaacgctg gagttaaccg gtactgagtt taccctgctc tatttgctgg cacagcatct
    22381 gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg ttgggcaaac gcctgacgcc
    22441 tttcgaccgc gctattgata tgcacatttc caacctgcgt cgtaaactgc cggatcgtaa
    22501 agatggtcac ccgtggttta aaaccttgcg tggtcgcggc tatctgatgg tttctgcttc
    22561 atgataggca gcttaaccgc gcgcatcttc gccatcttct ggctgacgct ggcgctggtg
    22621 ttgatgttgg ttttgatgtt acccaagctc gattcacgcc agatgaccga gcttctggat
    22681 agcgaacagc gtcaggggct gatgattgag cagcatgtcg aagcggagct ggcgaacgat
    22741 ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg cgattgataa gtgggcaccg
    22801 ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg tgatcggcgc tgaacgcagc
    22861 gaaatgcaga tcattcgtaa ctttattggt caggccgata acgccgatca tccgcagaag
    22921 aaaaagtatg gccgcgtgga actggtcggt ccgttctccg tgcgtgatgg cgaagataat
    22981 taccaacttt atctgattcg tccggccagc agttctcaat ccgatttcat taacttactg
    23041 tttgaccgcc cgctattact gctgattgtc accatgttgg tcagtacgcc gctgctgttg
    23101 tggttggcct ggagtctggc aaaaccggcg cgtaagctga aaaacgctgc cgatgaagtt
    23161 gcccagggaa acttacgcca gcacccggaa ctggaagcgg ggccacagga attccttgcc
    23221 gcaggtgcca gttttaacca gatggtcacc gcgctggagc gcatgatgac ctctcagcag
    23281 cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc tgacgcgtct gcaactgggt
    23341 acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac tcgagcgtat tgaaaccgaa
    23401 gcgcaacgtc tggacagcat gatcaacgat ctgttggtga tgtcacgtaa tcagcaaaaa
    23461 aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt ggagtgaagt gctggataac
    23521 gcggcgttcg aagccgagca aatgggcaag tcgttgacag ttaacttccc gcctgggccg
    23581 tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc tggaaaacat tgttcgtaat
    23641 gctctgcgtt attcccatac gaagattgaa gtgggctttg cggtagataa agacggtatc
    23701 accattacgg tggacgacga tggtcctggc gttagcccgg aagatcgcga acagattttc
    23761 cgtccgttct atcggaccga tgaagcacgc gatcgtgaat ctggcggtac aggattgggg
    23821 ctggcgattg ttgaaaccgc cattcagcag catcgtggct gggtgaaggc agaagacagc
    23881 ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt ataagcggag ttaaactccg
    23941 catttgtagg caggataagg cgtttacgcc gcatccggca tttgagcagg ataaggcgtt
    24001 tacgccgcat ccggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg
    24061 cctacactcc gggggttatt ttccccacaa ccggcgggaa aaatcttcga tcttgccgct
    24121 cagtcggcgc ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa
    24181 acggtgatac tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac
    24241 ggtcacatcg ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg
    24301 cgctatcacg ctatacaacc accccacctt acctgtgttt tgcatcaact gcgctatatc
    24361 gctgatatca aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat
    24421 taatgcctct ccccagcgga aaatatcgcc catatgaaca ttactttccg tgagaccgtc
    24481 ggttgagaga ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc
    24541 ccgcacccag tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccgcc
    24601 gtgaaccttt ttctccgcct gctcgtcacc ttccagcccc agctccgtca gcatcaactc
    24661 accatcaacc tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt
    24721 gcctgtgtat acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat
    24781 tagaccatat ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac
    24841 tcaatagcaa accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt
    24901 atttcaccgt acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga
    24961 tagaggtaag aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag
    25021 ttgctgccgg agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg
    25081 ctaccgcggc ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa
    25141 tcaatttatc ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca
    25201 ggatccccag caaaccagtc tgggcaatca cagtcaaatc aatggtattg cccagcgcga
    25261 aggcaaagaa tggaatcagc gtttgcaccg ctttgctgaa aaattctcgt aattcagggt
    25321 caaggttccc cagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg
    25381 gttcaaacga ggcaatccct gcagtgccca gaataatcat cgtcatgagc ggcccggact
    25441 ccaacgacat caacacaaat gccccagctt cttcttttgt gccgtactgc tgcatgatgg
    25501 aagcgtaaag tccgccattg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc
    25561 cggcaaagaa tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa
    25621 ccacccacgc gacggcaatt tttgttacca ccagcgtacc ggatttacgc aataccgttc
    25681 ccgtcgcgct taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca
    25741 ccgtaccggt aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga
    25801 aggtgtggca cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct
    25861 caatcgagcg ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg
    25921 tcattcctca atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat
    25981 aaaagtgatc aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt
    26041 tattttgaaa ttgatcacaa aaaaaccacc gccgttggcg atggttcatt ttttaagctg
    26101 atatgcggcc tatacgcctc attgcagcag gcggcaaatg attatttttt cgccgcaaaa
    26161 cgtgccgctg cttcgtccca gttcaccacg ttccagaact ctttaatgta gtccggacgg
    26221 cggttctgga atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag
    26281 ccggaagcgc cagaaatagc ttcacccatc agcggagaat cctggttagc agtagaaacc
    26341 accgccagtt tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct
    26401 gccgcttttt caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc
    26461 gctttcaggt caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg
    26521 ttagcgtgac cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc
    26581 agtttggtga tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc
    26641 gcgttggcgt tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc
    26701 ttatcgaagt gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc
    26761 atattcatct ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca
    26821 ttatagttaa ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat
    26881 ggttttgcag gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc
    26941 agtaaatcga caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc
    27001 atctcgtcgg agatgtgaag cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat
    27061 tacgatgggg atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc
    27121 gttcaaaaaa gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc
    27181 gtggattatt ctgccgtggg ccatcagcgc cctgttacta ccggatttct gggcgtatta
    27241 cagctcgttt agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat
    27301 cggtaatatc aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat
    27361 cgccattggc attacgttga ttgtcggtac actgatgacg ccaattatca acggcaattt
    27421 cgatgtgctg attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct
    27481 gattggcgta gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa
    27541 agccgaagag ttcaatctga aaaaagggct ggtgctggcg gttatgtgcg gcattttctc
    27601 tgccgggatg tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact
    27661 tggcgtcgat ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc
    27721 gatcattaac ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tgtcgctaaa
    27781 agccgacttc tcgctggcaa aaccgctgat cattcacaat gtgttactct cggcactggg
    27841 cgggttgatg tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc
    27901 gcagtatgac tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat
    27961 cgtcggactg gtgctgaaag agtggagcaa tgcaggacgc cgtccggtaa cggtgttgag
    28021 cctcggttgt gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta
    28081 atctttctgc gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa
    28141 caccaccgaa aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa
    28201 caggcggcta tgctggagaa gatattgcgc atgacacact ctgacctgtc gcagatattg
    28261 attgatggtc attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga
    28321 tgcctcatca caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt
    28381 aatcagctta tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg
    28441 atggcgattc agcaacatcg ccaactgccc gaacagcaac tctgccattt cgttagcaaa
    28501 cggcacatgc tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat
    28561 ccccacgcta cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc
    28621 cccctgccag tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac
    28681 cagatcgtta acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc
    28741 acgggtaatg cgataagggc gatcgttgag tacatgcagg ccattaccgc gccagacaat
    28801 caccagctca caaaaatcat gtgtatgttc agcaaagaca tgttgcggat aacggtcagc
    28861 cacagcgact gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg
    28921 cgccaccgtg gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa
    28981 tacgttgaga agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg
    29041 gtgaccagtt aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc
    29101 aatgataggc gatgtcagta acgctggcct cactgtggcg tagcagatgt cgggccttca
    29161 tcagtcgcag gcggttcagg taacgctgag gcgtcagtcc cgtttgctgc ttaagctgcc
    29221 gatgtagcgt tcgcagtgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat
    29281 cggcaaaatg gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca
    29341 ggttcttctg caaactgctt ttacgcagca agagcagtaa ttgcataaac aaagatctcg
    29401 cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc
    29461 tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc
    29521 agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga
    29581 tacagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg
    29641 cgtacgaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata
    29701 cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc
    29761 gcctgaggga gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca
    29821 ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg
    29881 aggtcaggtt cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca
    29941 agatacagcg tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagcaaat
    30001 tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa
    30061 cgagaaggtc acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca
    30121 cattatgacc tttcgcaatt gtgtcgccgt caatctcggc gcatccagtg ggcgcgtgat
    30181 gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa
    30241 aaatgggctg catagccaga acggttatgt cacctgggat gtggatagcc tggaaagtgc
    30301 cattcgcctt ggcttaaaca aagtgtgtga ggaagggatt cgtatcgata gcattgggat
    30361 tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc
    30421 cgttgcttat cgcgatagcc gcaccaatgg cctaatggcg caggcacagc aacaactcgg
    30481 caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca
    30541 gttgcgtgcg ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct
    30601 gatgccggat tacttcagtt atcgcctgac cggcaagatg aactgggagt acaccaatgc
    30661 caccaccaca caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg
    30721 gagcggggcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca
    30781 ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc
    30841 cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac
    30901 ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc
    30961 caacatcacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg
    31021 cttatggctg cttcagcgag tgcttcagga acggcaaatc aacgatctcc cggcgcttat
    31081 cgccgcgaca caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt
    31141 tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc
    31201 gatcccggaa agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta
    31261 tgccgatgtg ttgcatgagc tggcgcagct gcgcggtgaa gatttctcgc aactgcatat
    31321 tgtcggcggc ggctgccaga acacgctgct caaccagcta tgcgccgatg cctgcggtat
    31381 tcgggtgatc gccgggcctg ttgaagcctc aacgctcggc aatatcggca tccagttaat
    31441 gacgctggat gaactcaaca atgtggataa tttccgtcag gtcgtcagca ccaccgcgaa
    31501 tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg cgcagattca
    31561 ctctacacga cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag
    31621 ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt
    31681 gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac
    31741 ccggaaggtt cgctgaccgg tgggattcaa gctactggta attatccggg caaagcgcgt
    31801 aatgccagtg agctacgtac cgatctggaa caggctatgc ggctgattcc ggggccgaaa
    31861 cggcttaatt tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc
    31921 aaacctgagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat
    31981 tttaacccct cctgtttttc gcatccgcta agcgccgatg gctttacgct ttcccatgcc
    32041 gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc
    32101 tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg
    32161 aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag
    32221 gtgatcagcg agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt
    32281 ggcattggcg cggagagcta cacggttggc tccaatgagt tttacctggg gtatgccacc
    32341 agccgccaga cggcgctgtg cctggacgcc gggcacttcc acccgactga agtgatttcc
    32401 gacaagattt ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt cagtcgtccg
    32461 gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc aattgccagt
    32521 gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg gccttgactt cttcgatgcc
    32581 tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg
    32641 cgtgcgttgc tggaacctac cgccgagctg cgcaagttgg aagcggcggg cgattacact
    32701 gcgcgtcagg cactgctgga agagcagaaa tcgttgccgt ggcaggcggt atgggaaatg
    32761 tattgtcagc gtcacgatac cccagcgggt agcgaatggc tggagagcgt gcgggcttat
    32821 gagaaagaaa ttttgagtca gcgtgggtaa acactgccag atgcggcacg agcgccttat
    32881 ccggcctaca gatcgtgcat ctgacaacac cccatttaca ggaacaccga acatgcaaaa
    32941 cattactcag tcctggtttg tccagggaat gatcaaagcc accaccgacg cctggctgaa
    33001 aggctgggac gagcgcaacg gcggcaacct gacgctacgc ctggatgacg ctgacatcgc
    33061 accgtatcac gacaatttcc accaacaacc gcgttatatc ccgctcagcc aacccatgcc
    33121 tttactggca aatacaccgt ttatcgtcac cggctcgggc aaattcttcc gtaacgtcca
    33181 gcttgatcct gcggctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca
    33241 tattctctgg gggttaacca acgaggccgt tccaacttct gaacttccgg cacactttct
    33301 ctcccattgc gaacgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca
    33361 tgccaccaac ctgatcgccc tcacctatgt actggaaaac gacacggcgg tcttcactcg
    33421 ccaactgtgg gaaggcagca ccgagtgcct ggtggtattc ccggatggcg ttggcattct
    33481 gccgtggatg gtgcccggca cggacgaaat cggtcaggcg accgcacaag aaatgcaaaa
    33541 acattcgctg gtgttgtggc ccttccacgg tgtgttcggt tccggaccga cactggatga
    33601 agcctttggt ttgatagata ccgcagagaa atcagcggag gtgttagtca aaatctactc
    33661 gatgggcggt atgaagcaga ccattacccg tgaggagctg attgcactcg gtgagcgttt
    33721 tggcgttacg ccactggcca gtgcgctggc actttaatat cccaacagat atccggcggt
    33781 gtgggaaacg tcgccggaca gatggatttg aacttaagga gagtcattat gagttttatg
    33841 ttggcattac cgaaaattag cctgcatggt gctggggcaa ttggcgatat ggtaaatctg
    33901 gttgcgcata aacagtgggg aaaagcactg atcgttaccg atgggcaact ggttaagttg
    33961 ggattactgg acagcctgtt tatcgcactg gatcaatacg aaatgtctta tcacttattt
    34021 gatgaggtat ttcctaaccc cactgaagaa ctggtgcaaa aaggctacgc tgcatttcag
    34081 gatgccgcat gtgactacat tattgctttc ggtggcggta gcccaattga tactgcaaaa
    34141 gccgtaaaaa tccttaccgc taatcctgcg ccttctacgg cgtactctgg tgttggtaaa
    34201 gtgacaaatc ccggcgtacc gctggtggca atcaatacga ctgccggaac agctgctgag
    34261 atgaccagca atgcggtgat catcgacacc gcgcggcagg taaaagaggt cattatcgac
    34321 ccgaatatta ttcccgatat tgcggtggat gatgccagtg taatgctgga gatcccggcc
    34381 tcggttaccg ccgccacggg tatggatgca ctgactcacg cagttgaagc ttatgtttct
    34441 gttggtgcac atccattaac cgacgctaac gcactggaag ctatccgttt aattacgctg
    34501 tggctgccaa aagcggtaga tgatggtcat aatctggaag ctcgtgaaca aatggcattc
    34561 gcccagtacc tggcgggtat ggcatttaac agtgctggtc tgggccttgt tcatgcactt
    34621 gcgcatcagc ccggcgccac ccacaacctg ccgcatggtg tgtgcaatgc tatcttgttg
    34681 ccagttattg aaaactttaa ccgcccgaat gccatcgcac gctttgcccg cattgctcag
    34741 gcaatgggtg tcgatacgcg tgatatgagt gaagaagcgg caagtactga agcaattaac
    34801 gctatccgcg cattaagcca tcgcgtgggt attccggcag gattcagcca gcttggcatc
    34861 accaaagaag atattgaagg ctggttagat aaagccctgg ctgacccgtg tgtgccatgt
    34921 aacccgcgta ccgccagccg cgacgaggtg cgcgagctgt atctggaggc attatgatcc
    34981 gtaaggcatt tgtgatgcag gttaaccccg acgctcacga agagtatcaa cgtcggcata
    35041 accccatctg gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca
    35101 tttacctcga caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagagc
    35161 gctggaatgc ggttgccagt actgatgttt gccaacgttg gtggaaatat atgaccgacg
    35221 tcatgcccgc gaacccggat aacagcccgg tgagtagcga actgcaagaa gtgttttacc
    35281 tgccgtaatt gcctaatgtg ccggagacgc ctccggtaca atatttttaa tttcgatttg
    35341 aaaaataaac atattacacc ttcagttaat atatctcggt aacttatatt tccccgatgc
    35401 attgtgagta aatcacaaaa ataatgaata actcattaat gattcatgtg gtttatttaa
    35461 ataaccatta tgtgcattac tccgcaaatc tggcctttca ctctgtctca aattgtcatt
    35521 gtttaccgac atatcacctc tcatcactta atcgctccac cattacaatt gattaaaata
    35581 tattttatgg agtccgatta atggcagctc ttactgcaag ctgtattgac ctgaatattc
    35641 agggcaatgg cgcttattcc attctgaagc agttggcgac aatggcgtta caaaacggtt
    35701 ttatcaccga ctcacaccag ttcctgcaaa ccctgctgct gcgcgaaaaa atgcactcta
    35761 ctggatttgg ttccggcatc gccgtgccgc acggtaaaag cgcctgcgtt aaacaaccgt
    35821 tcgtgttatt cgcccgcaaa gcgcaggcca ttgactggaa agccagcgac ggcgaagacg
    35881 tcaattgctg gatctgcctc ggcgtgccgc aaagtggcga agaggatcag gtcaaaatca
    35941 tcggcacact gtgtcgcaaa attatccatc aggattttat caaccaactg cgacagggcg
    36001 acgccgacca ggtacttgct ttgttaaatc aaaccctcag ctcataagga agtggcgatg
    36061 gagtcatcct tacgtattgt cgcgatcacc aactgccccg ccgggatcgc tcacacctac
    36121 atggtggcgg aagccctgga acagaaagcc cgttctctcg gccataccat aaaagtggaa
    36181 actcaagggt ccagcggcgt tgaaaaccgc ttatccagcg aagagattgc cgctgccgat
    36241 tacgtcattc tagctaccgg gcgtggcctg agcggtgatg accgggcgcg gtttgccggg
    36301 aagaaagttt atgagattgc catctcccag gcgctgaaaa atatcgacca gattttcagc
    36361 gaattaccaa caaactcgca gctttttgcc gcagatagcg gcgtgaagct cggcaaacag
    36421 gaagtgcaaa gcggcagcgt gatgagccac ctgatggcgg gcgtctctgc ggcgctgcca
    36481 tttgtcatcg gcggcggtat tctggtggcg cttgccaaca tgctggtgca gttcggtttg
    36541 ccatataccg atatgtcgaa aggtgcgccg tcgtttacct gggtagttga atccatcggt
    36601 tacctcggct tcacctttat gatccccatc atgggcgctt atattgcctc gtcgattgcc
    36661 gataaacccg ctttcgctcc ggcatttctg gtttgctatc tggcgaacga taaagccctg
    36721 ctcggcaccc agtcaggcgc gggcttcctc ggcgcggtgg tgctggggct ggcgattggc
    36781 tatttcgtct tctggttccg taaagtccgt cttggcaaag cattacaacc gctgctcggc
    36841 tccatgctta tcccattcgt tactctgctg gtttttggcg tgctcactta ctacgttatc
    36901 gggccggtga tgtccgacct catgggcggg ctgctccact tcctgaatac cattcctcca
    36961 tcaatgaagt ttgcggcagc gtttctggtc ggtgcgatgc tggcgttcga tatgggcggc
    37021 cccatcaata aaaccgcctg gttcttctgc ttctcactgc tggaaaaaca catttatgac
    37081 tggtacgcca tcgtcggcgt tgtcgcgctg atgccgcccg tcgcggcggg tcttgcgacg
    37141 ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg ctgccagtag tgccattgtg
    37201 gtcggtgcca cagtcgcaac cgaaccggct attccttacg ctctggcagc tccgttgccg
    37261 atgattaccg ccaatacgct ggcgggcggt attaccggcg tgctggtgat cgctttcgga
    37321 atcaaacgtc tggcacctgg tttgggtatc tttgacccgc tgatcggcct gatgtcaccg
    37381 gtaggttcgt tttatctggt gctggcgatt ggcctcgcgc tgaacatttc attcattatc
    37441 gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg cgcaacagga gcttgtccat
    37501 gaacattgag ttactgcaac agttgtgcga agccagcgcc gttagcggcg atgaacagga
    37561 agttcgcgac attctgataa acacgctgga accttgcgtg aatgaaatca cctttgatgg
    37621 gctgggcagc tttgtcgccc gtaagggaaa taaagggccg aaagttgccg ttgtcgggca
    37681 tatggatgaa gtcggcttta tggtcaccca catcgacgag agcggttttc tgcgtttcac
    37741 caccattggt ggctggtgga atcagtcgat gctcaaccac cgggtaacca tacgcacaca
    37801 caagggagtg aaaatccctg gtgtgattgg ttccgtcgcg cctcatgcgt taacggaaaa
    37861 gcaaaagcaa cagccgcagt catttgatga gatgttcatt gatattggcg caaacagtcg
    37921 cgatgaggtg gaaaaacgcg gtgtggcgat gggcgatttt attagcccgg aagccaattt
    37981 tgcctgctgg ggcgaagata aagtggtcgg caaggcgttg gataaccgca tcggctgcgc
    38041 aatgatggct gagctactac agacggtgaa taatcccgaa attacgctgt atggcgttgg
    38101 cagtgtggaa gaagaagttg ggctacgcgg ggcgcaaacc tcggctgaac acattaaacc
    38161 ggatgtggtg atcgtgctgg acaccgctgt cgcgggcgat gttccaggca ttgataacat
    38221 taaatatccg ctgaaactgg gcaacgggcc ggggttgatg ctgtttgaca agcgctactt
    38281 ccccaaccag aaactggtag cagcgttaaa aagctgtgcc acgcagaatg atttaccgct
    38341 gcaattttcc accatgaaaa ccggtgcgac ggatggcggg cgctacaacg tgatgggcgg
    38401 tgggcgtccg gttgtcgcgc tgtgtttgcc aacgcgttat ctgcacgcca acagcggcat
    38461 gatttcaaaa gccgattacg atgctctgct cacgctgata cggggttttc tgaccacctt
    38521 aaccgcggaa aaagtcagcg cgtttagcca gttccgtcag gtggattaat gctaaacgaa
    38581 cgccagttaa agattgtcga tctgctggag caacagccgc gcacgcctgg cgagctggcg
    38641 caacagactg gcgtttctgg caggaccata ctgcgtgata ttgactatct caactttacc
    38701 cttaacggca aagcccgcat ttctgacagt ggtagtgcgg gctatcagct ggaaatcttc
    38761 gagcgccgca gcttttttca gttgctgcaa aagcacgata acgacgatcg gctgctggca
    38821 ctgttattac tgaatacctt tactccccgt gcgcaactcg cctcggcgct taatttgccg
    38881 gaaacgtggg tcgcagagcg tctgccccgg ttaaaacagc gttatgaacg cacttgctgc
    38941 ctggccagtc gccccggttt gggccatttc attgatgaga cagaagaaaa acgcgttata
    39001 ttgctggcga acttgctgcg caaagatccg tttttaattc cgctggcggg cgtaacacga
    39061 gacaaccttc agcatttatc cacggcctgc gacaaccaac accgctggcc gctcatgcag
    39121 ggtgattatc tctccagcct gattctggcg atttacgccc tgcgtaatca actgaccgat
    39181 gagtggccgc aatatcccgg tgacgagata aaacaaatcg ttgaacatag cggtctgttt
    39241 cttggtgata acgctgtaag aaccctgaca ggtttgatag agaaacagca tcagcaagcg
    39301 cagataattt ctgccgataa tgtgcagcgg ttgctgcaaa gggtgccggg catcgcgtca
    39361 ttgaatatta ttgatacgca gctggttgag aacattaccg ggcatttatt acgttgcctt
    39421 gccgcaccgg tgtggattgc tgagcaccgc caaagcagca tgaataacct gaaagccgcc
    39481 tggcctgcgg cctttgatat gagtctgcac tttattacgc tactgcgtga acagctcgat
    39541 attccccttt tcgacagcga tctgatcggt ttgtattttg cctgtgcgct ggaacggcat
    39601 caaaacgaac gccagccgat cattttgctc tcggaccaga acgcgattgc cactattaat
    39661 cagctcgcca ttgagcgcga tgttttacat tgtcgggtga ttattgcccg tagcttaagc
    39721 gaacttgttg ccattcgcga agagattgag ccgttattga tcattaacaa cagccattat
    39781 ttactggatg acgcggtaaa taattacatc accgtaaaaa atatcattac ggctgccggt
    39841 atcgaacaaa taaaacattt tctggcgacg gcatttattc gccaacagcc ggagcgtttt
    39901 ttctctgctc ccggaagttt tcattattcg aatgtacgcg gtgaaagctg gcaacatatt
    39961 acccggcaaa tttgtgcgca attagtcgca caacaccata ttaccgccga tgaagcacaa
    40021 cgcatcatcg cccgcgaagg cgaaggtgaa aacctgattg ttaatcgcct cgccatccca
    40081 cattgctgga gcgaacagga gcgacgtttt cgtggatttt ttattaccct cgcccaacca
    40141 gttgaggtga ataacgaagt cattaaccat gtcttgatcg cctgcgccgc cgccgatgcg
    40201 cgtcatgagc taaaaatatt tagctatctg gcaagcatat tgtgtcagca tccggcggag
    40261 attattgccg ggttaacagg atatgaggca tttatggagt tacttcacaa ggggtgaact
    40321 gtgtcggatg cgccgcatcc gacactgcat ttatcatcaa aaacgtttaa cgcaggctat
    40381 tatagtcatc aaccatttcg ttgtactcgc gtaatgctct cgggaaagag tcttcgacca
    40441 tccagcttga gtttgcgtcc tgaagttgct ctttatccca gtcagagtac gggactttat
    40501 ctctgatgcg gcgaacgtat tttttagcct cgagttgata ctggcctgcc gaattaataa
    40561 acgagaaatt catgccgcct ttatccgctt ctttggcctg cgccactagc gtttccagtt
    40621 cagacacttt cttcattgcc gcatcaacat caaacttctc ttgcgatatc aggttattaa
    40681 tttgtttcgc tgaaatcatg actgccagag agtagtagtg gaatgttttc ccttctcttt
    40741 cttcaatatt tttcagttcc gcaagctggc gcttatcgtt aatttcctga atcgccgcat
    40801 gataagattc tgctacaggt tcaaaggctt ccagattttt taagaatgtc tggtgcagcg
    40861 ttttcccttt cgcaaacgca tcgtctttat aattttcctg ggtgtaatat ttatccattt
    40921 cgttaatggt attacccaac gccacggcag catcgatata actcaccgcc acgccatcaa
    40981 ttggttgcag cgcaggcgtt aacgccacag cactttttac gcctttttca cactcagcga
    41041 ggttggattc actaatgccg tagatgccat aaacagtacg ctcttcaccg gtaggcccct
    41101 gtttaaaatc tttcagccag tcagcataac gcgccagact gctctgtaca gggatctgta
    41161 acttgttata gcacttgata tagacatcca ttttttcgtc gaaaaccgct tgttcatcga
    41221 ccaccggttg cgcaggtgtt tcaggtttag cttctgcttt tgcaacggga gcttcaggag
    41281 ctggtgcggc aggttgacta ttggcaggcg ggggcgtttc tgtttcggct tttttatcat
    41341 cacaacccgt cagaccgagg gccatgatgg cgactataat agcggaagat aataaattcc
    41401 ttttcat
//
LOCUS       DAZFKM010000027        41329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFKM010000027 DAZFKM010000000
VERSION     DAZFKM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..116)
                     /locus_tag="ACHKAY_001585"
     CDS             complement(<1..116)
                     /locus_tag="ACHKAY_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5700282.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQV"
     gene            complement(127..537)
                     /locus_tag="ACHKAY_001586"
     CDS             complement(127..537)
                     /locus_tag="ACHKAY_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5700283.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAALPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            complement(534..812)
                     /locus_tag="ACHKAY_001587"
     CDS             complement(534..812)
                     /locus_tag="ACHKAY_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700284.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(1101..2855)
                     /locus_tag="ACHKAY_001588"
     CDS             complement(1101..2855)
                     /locus_tag="ACHKAY_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO5700285.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLARERQNLSDGLVIELPVKQKAQLSQMADSERAQLLADRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            complement(2852..3151)
                     /locus_tag="ACHKAY_001589"
     CDS             complement(2852..3151)
                     /locus_tag="ACHKAY_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5700286.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSRKKARANIQEIKKMLNVFRGKK"
     gene            complement(3157..3390)
                     /locus_tag="ACHKAY_001590"
     CDS             complement(3157..3390)
                     /locus_tag="ACHKAY_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700287.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(3383..3616)
                     /locus_tag="ACHKAY_001591"
     CDS             complement(3383..3616)
                     /locus_tag="ACHKAY_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700288.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEAHYRRKFPEDVSHA"
     gene            complement(3609..3848)
                     /locus_tag="ACHKAY_001592"
     CDS             complement(3609..3848)
                     /locus_tag="ACHKAY_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700289.1"
                     /translation="MCNNTRPDAAAEAIITLMHALIDISVIADRAHKHDTSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGYA"
     gene            complement(3838..4044)
                     /locus_tag="ACHKAY_001593"
     CDS             complement(3838..4044)
                     /locus_tag="ACHKAY_001593"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5700290.1"
                     /translation="MRNHTTHPQGRDSHDLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQEVCHVQ"
     gene            4244..4423
                     /locus_tag="ACHKAY_001594"
     CDS             4244..4423
                     /locus_tag="ACHKAY_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5700291.1"
                     /translation="MATGTTNAKSQALKARVPHEIVNAMESVKESGESTSQFIIASMQ
                     GEIKRRQRRKAKESE"
     gene            complement(4426..4866)
                     /locus_tag="ACHKAY_001595"
                     /pseudo
     CDS             complement(4426..4866)
                     /locus_tag="ACHKAY_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4924..5112)
                     /locus_tag="ACHKAY_001596"
     CDS             complement(4924..5112)
                     /locus_tag="ACHKAY_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5700292.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(5487..6716)
                     /locus_tag="ACHKAY_001597"
     CDS             complement(5487..6716)
                     /locus_tag="ACHKAY_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5700293.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEAKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            6704..6886
                     /locus_tag="ACHKAY_001598"
     CDS             6704..6886
                     /locus_tag="ACHKAY_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700294.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            6893..7798
                     /gene="tqsA"
                     /locus_tag="ACHKAY_001599"
                     /pseudo
     CDS             6893..7798
                     /gene="tqsA"
                     /locus_tag="ACHKAY_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(7823..9211)
                     /gene="pntB"
                     /locus_tag="ACHKAY_001600"
     CDS             complement(7823..9211)
                     /gene="pntB"
                     /locus_tag="ACHKAY_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO5700295.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(9222..10754)
                     /gene="pntA"
                     /locus_tag="ACHKAY_001601"
     CDS             complement(9222..10754)
                     /gene="pntA"
                     /locus_tag="ACHKAY_001601"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO5700296.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            11278..12222
                     /gene="ydgH"
                     /locus_tag="ACHKAY_001602"
     CDS             11278..12222
                     /gene="ydgH"
                     /locus_tag="ACHKAY_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO5700297.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            12408..13790
                     /gene="ydgI"
                     /locus_tag="ACHKAY_001603"
     CDS             12408..13790
                     /gene="ydgI"
                     /locus_tag="ACHKAY_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5700298.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            13827..14549
                     /gene="folM"
                     /locus_tag="ACHKAY_001604"
     CDS             13827..14549
                     /gene="folM"
                     /locus_tag="ACHKAY_001604"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO5700299.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(14546..14881)
                     /gene="ydgC"
                     /locus_tag="ACHKAY_001605"
     CDS             complement(14546..14881)
                     /gene="ydgC"
                     /locus_tag="ACHKAY_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO5700300.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            15010..15729
                     /gene="rstA"
                     /locus_tag="ACHKAY_001606"
     CDS             15010..15729
                     /gene="rstA"
                     /locus_tag="ACHKAY_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO5700301.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            15733..17034
                     /gene="rstB"
                     /locus_tag="ACHKAY_001607"
     CDS             15733..17034
                     /gene="rstB"
                     /locus_tag="ACHKAY_001607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO5700302.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            17110..18039
                     /gene="tus"
                     /locus_tag="ACHKAY_001608"
     CDS             17110..18039
                     /gene="tus"
                     /locus_tag="ACHKAY_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO5700303.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(18036..19439)
                     /gene="fumC"
                     /locus_tag="ACHKAY_001609"
     CDS             complement(18036..19439)
                     /gene="fumC"
                     /locus_tag="ACHKAY_001609"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO5700304.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(19582..21228)
                     /gene="fumB"
                     /locus_tag="ACHKAY_001610"
     CDS             complement(19582..21228)
                     /gene="fumB"
                     /locus_tag="ACHKAY_001610"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5700305.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21427..22602
                     /gene="manA"
                     /locus_tag="ACHKAY_001611"
     CDS             21427..22602
                     /gene="manA"
                     /locus_tag="ACHKAY_001611"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO5700306.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            22703..24211
                     /gene="ydgA"
                     /locus_tag="ACHKAY_001612"
     CDS             22703..24211
                     /gene="ydgA"
                     /locus_tag="ACHKAY_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5700307.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(24256..25521)
                     /gene="uidC"
                     /locus_tag="ACHKAY_001613"
     CDS             complement(24256..25521)
                     /gene="uidC"
                     /locus_tag="ACHKAY_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO5700308.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(25560..26933)
                     /gene="uidB"
                     /locus_tag="ACHKAY_001614"
     CDS             complement(25560..26933)
                     /gene="uidB"
                     /locus_tag="ACHKAY_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO5700309.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(26930..28741)
                     /gene="uidA"
                     /locus_tag="ACHKAY_001615"
     CDS             complement(26930..28741)
                     /gene="uidA"
                     /locus_tag="ACHKAY_001615"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO5700310.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(29130..29720)
                     /gene="uidR"
                     /locus_tag="ACHKAY_001616"
     CDS             complement(29130..29720)
                     /gene="uidR"
                     /locus_tag="ACHKAY_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO5700311.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(29941..30708)
                     /gene="hdhA"
                     /locus_tag="ACHKAY_001617"
     CDS             complement(29941..30708)
                     /gene="hdhA"
                     /locus_tag="ACHKAY_001617"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO5700312.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(30820..31848)
                     /gene="malI"
                     /locus_tag="ACHKAY_001618"
     CDS             complement(30820..31848)
                     /gene="malI"
                     /locus_tag="ACHKAY_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO5700313.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            32023..33615
                     /gene="malX"
                     /locus_tag="ACHKAY_001619"
     CDS             32023..33615
                     /gene="malX"
                     /locus_tag="ACHKAY_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO5700314.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            33625..34797
                     /gene="malY"
                     /locus_tag="ACHKAY_001620"
     CDS             33625..34797
                     /gene="malY"
                     /locus_tag="ACHKAY_001620"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO5700315.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            34901..35902
                     /gene="add"
                     /locus_tag="ACHKAY_001621"
     CDS             34901..35902
                     /gene="add"
                     /locus_tag="ACHKAY_001621"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO5700316.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(35937..36977)
                     /gene="ydgJ"
                     /locus_tag="ACHKAY_001622"
     CDS             complement(35937..36977)
                     /gene="ydgJ"
                     /locus_tag="ACHKAY_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5700317.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            37220..37345
                     /gene="blr"
                     /locus_tag="ACHKAY_001623"
     CDS             37220..37345
                     /gene="blr"
                     /locus_tag="ACHKAY_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO5700318.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            37618..37833
                     /gene="ydgT"
                     /locus_tag="ACHKAY_001624"
     CDS             37618..37833
                     /gene="ydgT"
                     /locus_tag="ACHKAY_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO5700319.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            37919..38359
                     /gene="ydgK"
                     /locus_tag="ACHKAY_001625"
     CDS             37919..38359
                     /gene="ydgK"
                     /locus_tag="ACHKAY_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO5700320.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            38436..39017
                     /gene="rsxA"
                     /locus_tag="ACHKAY_001626"
     CDS             38436..39017
                     /gene="rsxA"
                     /locus_tag="ACHKAY_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO5700321.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            39017..39595
                     /gene="rsxB"
                     /locus_tag="ACHKAY_001627"
     CDS             39017..39595
                     /gene="rsxB"
                     /locus_tag="ACHKAY_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO5700322.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            39588..>41329
                     /gene="rsxC"
                     /locus_tag="ACHKAY_001628"
     CDS             39588..>41329
                     /gene="rsxC"
                     /locus_tag="ACHKAY_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO5700323.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEP"
BASE COUNT         9914 a        10789 c        10007 g        10619 t
ORIGIN      
        1 acctgatgcc gttccttgtc tgtctccacc agcagcatga cttcacccca tcgcgccgcc
       61 gccctccggt aaaagccttt cgcctcgagt tcttccgcta tgcggtcatg taccatcgcc
      121 accccctcag aacggaatac cgtcaccgta agggtcatcg cctcccgctg gtggctgatt
      181 accctgtgtg cctgtggttt tgcgtctgtt cccgccagga agtgccgcac gggcactgat
      241 tacgctgtct gcaataacct gataaccctg ccgcgtttcc ccattctgtc cggtccactg
      301 gctgacctgc atcgtgccgg atacgctggc aacgtcgcct ttttgatgtt tagccaggaa
      361 gtcggcctgc ttaccaaatg cgatgaccga tagccataac gtcgcctgcc catcctgcgc
      421 ctgactacat ggcagcgata ccgccatacg cgccagcgtc atcggtgtac ccttgctggt
      481 ctgttttacc tgcgggtcgt ccaccagccg cccgtaagct gctatctgtg ctgtcatgat
      541 tccacctctc cggttttaac gttgatggtt gttacctgtt ccgcttcggc aatctcccgc
      601 tctgtcagcg tggcaaaatt tgccgccgtc gtggtcatga atgcgcttat cagttcggga
      661 tgtgctttcg cgtatccttc ccccgcgtgg cggtctatcg ttctgattgc cacctttaaa
      721 gcgtgctctg tcatgtctaa cgcgcgatat ttcggttctg ttctgtctct gcgttttttg
      781 agtgatttat taaatttccc tgaagtgtgc atatttattt ttaccccctc gtttaaaaag
      841 ttttgagttg tgcctcccct tgtctacctt atctacctta gtggccctca taccagtaat
      901 ggcgcggctt tcagctgggt agagtgcttt tatccactat ctaccccgtg tctacctccc
      961 tgtctgattc aggtaaaatc aggtagagag ggtagatagt gggtagacag taaaaaaagg
     1021 ctatctacct aacttaatgc actgaattaa atgtattttt ctttactcag gtagacaggg
     1081 tagacagcaa ttacaaaaaa ttataaaaac gcgtcgcact cgtctgttgt tattgcgtta
     1141 gtctgcgtta ctcccttaac tttccgcgta atatattcat gtccgtaaac tttcgcggct
     1201 ggcttcatag ccttgccaaa gtcatttacg tttagcggtt tgctcctgcc tgcgtacgcc
     1261 ataaacgcca gatagacgcg gtaaaggctg tttctggtcg tgtacttcac tgaatcgcca
     1321 ccgccgccca tcatcaggcc gcgtgcttcc tccagaaaat tcaggaactg gcaaaactca
     1381 ataaccggat ccgtctgttg ctttattgcc agagcttcat caccgtcacg ctgttccagt
     1441 agtaaagccc gtgccttctc agggtcggta aagttcgcca gcaatcggcg gataatgaca
     1501 gggatttcag ccgcgatctt ttccggtagc tccctgtctt tttcggcctc actgacgata
     1561 ttgtcgaaac ggaaaatcac gcgacgacgt gccacacctc cggcccgttc ggtgaatatc
     1621 atcgggttat tgttggtcgc cagcaccacc gccctgatta cagccgtgaa acgcttttcg
     1681 tatttcgggt taatttccac ggggtcaccg cccgtgattt tcttgatgcc cgttccttcg
     1741 cctgtatatt tcggctggtc agccaggacg ataagacgac tcccgacaac ctgcgcacgt
     1801 ccaccagcat catcaagcga tgtcatttca gcgcttaccg tgttctgttt ccctgccaga
     1861 aggctggcta tgtgcgtgaa tgtactttta ccgctcccgc cgtctccggt ggcctcaata
     1921 aacatctgcc agtcgtaccg gttcgccata atcatgtaca gcgcggcaca tatacgcatc
     1981 atcttgcgcg ggtcttttcc ggctgcgtgc tcaagccatt tatgaaagtt tggcgcgtta
     2041 tcgcggatgt tctcccctgg tgctggtggc gtgtactcaa tgccgttgtg cgtggtgatc
     2101 cagttctccg gcgtgtgcgg ggaaaattcc cccgttttca ggtcaagcgc accattggcg
     2161 aacggcagca aatcgccgga cggctcgccc attggttcgg caataacttt taacgcttcc
     2221 acggcgttat tgattacgcg cttgctgaaa gtggccctgt gctctgaata gatcgccacc
     2281 atttcgcggc taagttccat tgtgctgacc ggacaccata ccccgccgcg ccatacgtga
     2341 acgatttcac tttcaggatg tacgcaaacg ccatcaaagc gatcggcaag cagctgcgcg
     2401 cgctcactgt ccgccatctg cgaaagttgc gccttttgct ttaccggaag ctcaatgacc
     2461 agaccatcag aaagattctg gcgctcacga gccagatatt cgcgccagtt ctccaccttc
     2521 tgaccgtgca taccatcagg gtaaaaattt gcatcctgta cgcctgccgc cgccagcttc
     2581 tgaccaatcg cctttatcat tactggcgca agatatccgg ccctgaatat gcgcacggat
     2641 tttctgcctt ccggcacaat ttgcagctta tccagttcgg ataactgctg ctccccaagc
     2701 cacacaggag gctcattatc tccggccata cgcgcgtcat gttcctgcca ttgttttgcg
     2761 tgtgaccagg catcactacc cgcaaaaata atgacttctg tttctttgtg ttttatgccg
     2821 cgtgactgct gttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
     2881 tcttttttat ttcctgaata ttggcgcgtg ctttcttcct gctggttggt ttactgcggg
     2941 gcgctgcctg taccagagaa aaatcacgcc gaaactgata aacaggcatc acgcaatcat
     3001 attcgtagcc ttcacggcgg taggttacgc gccgttcttc cacgccctta atcattaccg
     3061 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
     3121 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gctgttcttt
     3181 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
     3241 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
     3301 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
     3361 gtatattgtt tcgtggtaaa tatcacgcat ggcttacatc ctcaggaaat tttcttctgt
     3421 aatgcgcctc tgccacatat tccgcataat cggcggcgat attcagtaca tcaagccccg
     3481 ttgatttata ttctctcgtg gaaagtaaga aaaaagccgc tctaataagc tctggcattg
     3541 acgaaagcgc atcagccgca tcatcaggaa cgccggaaaa ttcctgtttc agggaattaa
     3601 aacgatcatc acgcataacc acccccattt tcacaatcag caatcaggat ggctttagcc
     3661 tcattcagcg ccatatcagc actaagttgc ataaccgcca gcgaatgagg aacgaaagcc
     3721 ccggcatatt ctgtttcact ggtgtcgtgc ttatgcgccc tgtcagcaat aacagaaata
     3781 tcaatcagcg catgcattag cgttatgatg gcttcggcgg ctgcgtccgg acgggtgtta
     3841 ttgcacatgg cacacctcct gacgaatacg ggcagcgaat accatcacgc agccagccgg
     3901 agattgctgg cgtgcttcct gttcgctggt ggcctcaatg gtaatcacgc gcggttgtgc
     3961 cgtgctcagg gcgataaaac gccagatgta tttattcagg tcgtgcgagt cccgcccttg
     4021 cgggtgtgtg gtatgatttc tcatagctac ctcgatactt tcgctatcgt tggtggttag
     4081 aagccctgcg agtggtaacg acacttgcgg ggctttgcat ttatgcacct tgataatctc
     4141 aaggtgtggc ccactatatg ctttaggtgt ggcccacatc aagggtttta tttgtgcttt
     4201 ttctgtgtat actgtccccc accaatccaa cagaggaata gaaatggcaa cgggtacaac
     4261 aaacgcaaaa tcacaagctc taaaggctcg tgtaccacac gaaatagtaa acgccatgga
     4321 atcagtgaaa gaatcaggcg aaagcacatc acaattcatc attgcgtcaa tgcaaggcga
     4381 gatcaaacgc cgccagcgcc gcaaggccaa agaatcagaa taatcactat cagcgccgtg
     4441 gtgtgaggta ttacggcgca ttgctatgca ggacaacaca atgaccgata aagaattgac
     4501 caaaacatta tcaccggcac ggaaaagacg gcgcagaaag atagaacatg aatcagaaag
     4561 attcgcgcca tgtgcttttg cccttgagca attccttaaa gagtacaggg aaaagcgctc
     4621 attgcaggta tggcaacgaa ctgaaccaga ctgattgcat tgcccaccag cctgatagcg
     4681 gctatcatcc ccgtgcttat gtttgggatc acacacacaa acggcgcagc gggttatctg
     4741 ttcagaaagg cggctccatt tcggggccgc tttttttatg cctgaaaaac cccaattttg
     4801 tggttttcca gtctcaccag ggcgaacgaa tccccgccca cgttctggcg tatattcaat
     4861 cttcatggtt atagctctgt gttcagatga ttgatgtgtg gcggctgtgt gccgccagcg
     4921 tgattaatga actgccttgc agctatcctt ccaggccaga acctcggata aagaccagcc
     4981 aacggaacga ccgccaagtt tacgacgtga tgggaattgt ccggcctttt ccaggcggta
     5041 gcggcatgag cggctaaggc ctgttagctt ttcgcattct ttttcacgta taaaccgatc
     5101 agtgcttaac actattgccc cctttcgttt cttaaagagt tatttcgtgt tctattgcgt
     5161 tgggatgtgt ctgattgtgt caggatgatt cagagttggc aaatgttgag gtcgtatggt
     5221 ttacagaaac aggaataatc aggataaaat catttaaatt catgttaata caaaggcata
     5281 aaatattgtt tcatgccttt tttctcgcca tttaaagagt gattcgctag tgtataaaaa
     5341 accagtaaca cattaaaaat cagccactta taaatccgta cactttttcg cctcttgttc
     5401 gtagttgttc cgcattgttg ctcattgttg catgttgtat ctgttcgcat atccagtatg
     5461 cgcatactga aaaaacacga aaaaaattat tttcttctgg ctactggtag cgtggttacg
     5521 ttttcatgtg ttcccgccag tatccctaac cgctccgtcc acatatccag cgcattgcgt
     5581 ttagcatcca gataacggga atgattataa actcgctgca ttcctggcat ctgatgacct
     5641 gtaagctgct ccacgacatg cggatcaacg cctaaatcgt tcagcatggt tgtaaaggtg
     5701 cgccggatgt catgcagtga ccagtgagga tgattaagcc tcctgtgcgc taatcttcca
     5761 tactgcgata cgctggcctc ctgtttcgct tcccccagca ataagcccgt gtgcctgttc
     5821 tgctccacca gctgcgtgac gaacggcagg atcgcttccg gtatgggccg gaatattgcg
     5881 accttcgttt tgctgtgctc cttcggaacg gtccatagca tttccgtaaa atcccactcc
     5941 ccgatctccg atagcctcag ttctaccgtc ctggctccga agacaatcag gaggcggatt
     6001 aacgcgacgt agtaagggga aaatattttt ttgtccagtg cctgcaataa ttcgccaagt
     6061 tctttgttac ttaagacacg ttcgcttata tccggttttt tcccaacgtc cgccacgttc
     6121 agatcgtcca gaacgttgct gattgcatag cgccgcctac ggcagaactt aagcgcctgt
     6181 ttgcacgcct gtagcacgaa tccggcagta acaggcgttc gctttgccac ctggtcaaaa
     6241 caggccagcc agtgccgtag ctcgcattta tccagcggca tagcaccaat ctgctgtatt
     6301 acgtgattat taagtcgcct tttcagggcg atataatcca cgcggttttc ctttacgtaa
     6361 tactcaagcc agtaggtgag cgcatcgcca accgttacgg gctttaacgc ttcctgtacg
     6421 gtgtaattca tctcatgacg tggatttttc ccctcagcca gccatgtgcg acactgtgcg
     6481 gctttttccc tggctgcttt caggctcaga tcaggataac ttcccagctt aatgcgttcg
     6541 ggtcgtgtct cccttcccgt tccggccctg tatgtgaaat accaggtcaa tttccctgat
     6601 gttaaatatt tcacgctcag gtttccgcca tcactataaa acgtgttttt ctccgctggc
     6661 ttaccatgaa gtttccttag caaggtatcg ctcagtttgt tcattgctct gctcacgttt
     6721 taccccctct ttacgaaaaa gtgactacac cagtgactac accgatcgtt gcacaaaggg
     6781 ctacaacgtg aaacaagctg gaacaaggaa aatcacaaat ctgttgataa tcaaaaacat
     6841 aatgcacagc ctgaaacatt atgaaacagc aaaaaacact aaatgataaa atgctgttat
     6901 tcttaacccg ctggtgcaac acatggtccg ctggcgagtg ccgcgtgtac tggcggtgtc
     6961 gattttgatg accatcatcg tgatggcgat ggtgttgcta ttagcttatc tgggttccgc
     7021 gctcaacgag ttgacgcgga cgttaccgca atatcgcaac tctattatga cgccgctgca
     7081 agctcttgag ccgttgttgc aacgcgtagg gattgacgtc tcagttgacc agctggcgca
     7141 ttacattgat ccgaacgcgg cgatgacgtt gctcaccaac ttattgacgc agttatctaa
     7201 tgccatgtca tcaatatttt tattgctgct gacggtgctg tttatgctgc tcgaagtgcc
     7261 acaattgccc ggaaaatttc agcaaatgat ggcgcgtccg gttgaaggga tggcggcgat
     7321 tcaacgtgca attgacagtg tttctcatta tctggtgctg aaaacagcca tcagcatcat
     7381 caccggcctg gtcgcctggg cgatgctcgc cgcactcgat gttcgcttcg cttttgtctg
     7441 gggattgctg gcctttgcgc ttaattacat ccctaatatt ggttcagttc tcgcggcaat
     7501 cccccctatc gctcaggtac tggtgtttaa tggcttctac gaagcgttgc tggtgctggc
     7561 gggatatctg ctgattaatc tggtcttcgg caatattctg gagccgcgca tcatgggacg
     7621 tgggctgggg ctttccacat tggtggtatt tttgtcgttg attttttggg gatggttgtt
     7681 aggaccggtg ggtatgctgc tttccgtgcc gttaacaatt attgtcaaaa ttgcgcttga
     7741 acaaacagcg ggaggtcaaa gcatcgccgt tctgttaagc gatctcaata aagagtgacg
     7801 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
     7861 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     7921 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     7981 caggcatacc agcaatagga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     8041 tagcaccaat caccagtacg gtatcggtat cagcaaagtc atcgttgatc tcgtccattt
     8101 ccagcacgat gtcatacggt acttttgctt cagccagcaa tacgttcata tgtccaggca
     8161 aacgccccgc aaccgggtgg ataccgaaac gcacgttgat accacgagcg cgcagtttct
     8221 cggtaatttc agcgacagga tattgcgcct gcgcgactgc catgccgtac cccggagtaa
     8281 tgatcactga atgggagttt ttcagcagtt ccgctgtctc ttctgcggtg atttcgcggt
     8341 gctcgcccac ttcctgatca tcgccagtag aagagccgtc ggtgccgaaa ccacccgcaa
     8401 taacgctgat aaaggaacgg ttcatcgcct tacacataat gtaagaaagg atagcgcccg
     8461 aagaaccgac cagcgcaccg gtcacaatca gcaggtcgtt gctgagcata aagcccgcag
     8521 ccgcagccgc ccagccggag tacgagttca gcatcgacac caccactggc atatctgcac
     8581 caccgatgga ggcgactaaa tgccagccga ataccagcgc aatggcggtc attatcagca
     8641 atgccagcac ttgcaggccg acgctatccg tgcgaacaaa tacaatcagc agcaggaagg
     8701 aaacgaccag agccgccagg ttcattttgt gacggtttgg cagcatcaac ggtttagacg
     8761 aaatcttgcc acacagtttg ccgaacgcca ccaccgaacc cgtgaacgtt accgccccga
     8821 tgaagatacc gaggaacact tccgtcaggt gaatattgac cagaatcggt gccattcccg
     8881 cgtcatgatg cagataactg ttaaagccaa ccagcactgc cgccagaccc acgaagctat
     8941 gcaggatcgc caccagttct ggcatttcgg tcatttcaac tttcttcgcc agacggatac
     9001 caattgcccc accaatgacc atcgccagca agatccagcc aacattaccc gtatccggtc
     9061 caaaaatggt tgcgattaac gcaatcgcca tcccggcgat accgaagttg ttaccctggc
     9121 gagacgtttc atgtttcgaa agaccggcca gactgaagat aaacaggatc gcggcaacaa
     9181 tgtatgcagc tgtaactaat cctccagaca tatgttaccc cttaattttt gcggaacatt
     9241 ttcagcatgc gctgagtcac ggtgaagcca ccgaaaatat taatgctggc tataagcacc
     9301 gcgataaaac taaggaagct aacccagccg ccctggccaa tctgcaacag tgctccgaca
     9361 acaataatcc ctgaaatcgc gttggtgacc gacatcaacg gtgtatgcag cgcatgcgat
     9421 acattccaca ccacgtaata accgacaacg caggccagcg cgaaaacggt gaagtgccca
     9481 agaaactctt tcggcgcaac gcttgccagc caaccgaaaa ggatgattgc cagcgccatc
     9541 aatgcgtatt tacgccacgg tgagcaggca catttttcct cagttttcac ttccggtgcc
     9601 gctttttgtg ccgcctgcgg ctgagctgat acctgaatcg gcggtgccgg ccaggtaatt
     9661 tcgcccgcac ggatcacggt cacgccgcga atcaccacat catcaaaatc aacagtgata
     9721 ttgccgtctt tctctttgca caacagtttc agcagattaa cgaggttagt accgtaaagc
     9781 tgtgaggatt gcgtcggcag acggcccgga agatcggtat aaccaatcac tttgacacca
     9841 ttttccgtag tgaagatttc acccggcacg gtgtattcac agttgccacc gttttgggct
     9901 gccaggtcga caatcacact gcccgccttc atggagtcaa ccatttcacg ggtaattagc
     9961 ttcggcgctg gtttgcctgg aataagcgcg gtggtgacaa tgatatcgac ctcttttgcc
    10021 tgggcggcaa agagttccat ttccgctttg atgaacgcgt ccgacatcac tttggcatag
    10081 ccatcgccgc tgcccgcttc ctctttaaaa tccagctcga ggaattccgc gcccatactt
    10141 tgaacttgtt ctttcacttc cgggcgggtg tcgaatgcac gcacaatcgc gccgagactg
    10201 tttgctgcgc caatggcggc cagacctgca acacccgcac caatcaccat cacttttgcc
    10261 ggtggcactt tcccggccgc agtaatttgc ccggtaaaga agcgcccaaa ttcatgtgcc
    10321 gcttcaacaa tggcgcgata accggcgatg ttcgccatcg agcttagtgc gtccagcgat
    10381 tgtgcgcgtg agatacgcgg cacagaatcc atcgccatca cggtcacgtt acgttccgca
    10441 agtttttgca ttaattccgg attctgcgca ggccagataa aactcaccag cgttgtccca
    10501 ggattcagta acgcaatttc atcatctaac ggcgcattga ccttcagaat gatctctgac
    10561 tgccagacgc tattcccttc tacaatttca gcgcccgctt gcacaaacgc tttatcgtca
    10621 aaacttgcca gttgacccgc gccgctctct accgcgacgg taaaacccag tttcagcagc
    10681 tgttccactg tttttggcgt tgctgcaaca cgggtttcat tggttaaccg ttctcttggt
    10741 atgccaattc gcatgatatt cccttccatc ggttttattg atgatggttt gcctgtgtca
    10801 ggagccacac aagccgctca tgtacgagct aaatgttact ccgttaaaat aaattagtaa
    10861 caaacgccct ataacgtact gaaaattatg cctgtgatct agcgccaaaa atcagtattt
    10921 cggcgtgaac tcgcaaaata ttaacgattc agccgtgata gtgggataaa caccttagaa
    10981 cgccggataa agactgataa ttgtcttcga cggtcgggta aaacgagaca tcgccccggc
    11041 acgaatcact acttaacatt aaattaactt atacaattca gttgcttcag tagtaatgat
    11101 gctgatacgg ctgtttttta agcatagacg gtcatttgag caggattaaa attggcttaa
    11161 ggaatgtgat atgaaaaatg acgcagacag ttacaccgtt taaatgcaat aatcagccac
    11221 gtttctcgtt aataacaata ccagtacctg gtttgcgcaa ggcgaaggat tatttttatg
    11281 aagcttaaga acaccctcct ggcgtcggca ctgctttctg ctacggcatt ctccgttaac
    11341 gcagcaacag aactgacacc ggagcaagcg gcagcggtta aaccttttga ccgtgtagtg
    11401 gttaccggtc gttttaatgc tattggcgaa gcggtgaaag ccgtttctcg tcgcgcagat
    11461 aaagaaggtg ccgcctcttt ttatgttgtc gacacttctg attttggtaa cagcggtaac
    11521 tggcgtgtgg tcgctgacct ctataaagcc gatgctgaaa aagcagaaga aacaagtaat
    11581 cgcgtaatta acggtgttgt cgaactgccg aaagatcagg ctgttctgat tgaaccgttt
    11641 gacacggtca ccgtccaggg cttctatcgt agccagccag aagtcaatga tgccatcacc
    11701 aaagcagcaa aagcgaaagg tgcctactct ttctacattg ttcgtcaaat cgatgccaac
    11761 cagggcggca accagcgtat tactgcattc atctataaaa aagatgctaa gaaacgtatc
    11821 gtccagagcc cggatgtgat cccggcagat tccgaagcag gacgtgcagc tctggctgcc
    11881 ggtggcgaag ccgcgaagaa agttgagatc ccaggtgttg cgactaccgc atcaccaagt
    11941 tctgaagtcg gtcgtttctt tgaaacccag tcatcaaaag gcggacgtta cactgtcacg
    12001 cttcctgacg gcactaaagt cgaagaactg aacaaagcga cagcagcgat gatggtcccg
    12061 ttcgacagca tcaaattctc tggcaactac ggcaacatga ccgaagtctc ctatcaggtt
    12121 gcgaaacgtg ccgcgaagaa aggtgctaag tattaccaca tcacccgcca gtggcaggaa
    12181 cgtggtaata acctgaccgt cagcgcagat ctgtataaat aactattcag ttactcctat
    12241 aaggcggctt gatgccgcct ttcgcatttt tagtcacaaa tttccccgcg accattgcat
    12301 gcagacacat ctcttcgtaa aatcccgcgc ttcagtggca aactccattt ttatgcgttt
    12361 tgccctaata tttattcttc ttatcacgtt ttaatcactg gatatcgatg gaaaagaaac
    12421 tgggactgag cgcactcacc gcgctggtat taagctcaat gctgggcgcg ggtgttttca
    12481 gtctgccgca aaatatggcg gcagttgcca gcccggcagc actgctcatc ggctggggta
    12541 ttactggcgc tggcatttta ttgctggcct ttgccatgct gatcctcacg cgcattcgtc
    12601 ccgaacttga cggcggtatc tttacctatg cccgcgaagg gtttggcgag ctaatcggct
    12661 tttgttccgc atggggatac tggctgtgcg cagtcatcgc caacgtctcc tatctggtga
    12721 tcgttttttc cgcgttaagc ttttttacgg acacgccgga attgcgcctg ttcggcgatg
    12781 gtaatacctg gcaatcgata gtcggtgcat cggctttatt gtggattgtt cactttttga
    12841 tcctgcgcgg ggtgcaaacc gctgccagca ttaacctggt ggcgacactg gcaaaattgt
    12901 tgccgctggg tctgtttgtt gtgctggcga tgatgatgtt caaactggat accttcaagc
    12961 tcgacttcac cggacttgca cttggcgtac ccgtgtggga acaggtgaaa aacaccatgc
    13021 tgatcaccct gtgggttttc attggtgtgg aaggtgccgt ggttgtttct gcgcgtgcgc
    13081 gtaataaacg tgatgttggc aaagcgacac tgctggcggt tctctccgct ctgggcgttt
    13141 acttgctggt aacgctgctt tcgctgggcg tggtggcacg tcctgaactg gctgaaattc
    13201 gtaacccgtc aatggccggt ctgatggtgg aaatgatggg accatggggc gaaatcatca
    13261 tcgctgccgg tttgatcgtt tccgtttgcg gcgcgtactt aagctggacc atcatggcgg
    13321 cagaagttcc gttcctggct gccactcata aagcattccc acgcattttt gcgcgccaga
    13381 acgcacaagc tgcgccatcc gcctcgctgt ggttgaccaa catctgcgtg caaatttgtc
    13441 tggtactcat ctggctgacc ggttccgatt acaacacgtt gctgaccatc gcctcagaaa
    13501 tgattctggt gccctatttc ctggtcggcg cgttcttgct gaaaatcgca acccgtccac
    13561 tgcataaagc ggtaggtgtc ggtgcctgca tttatggctt atggttattg tatgcctctg
    13621 gtccaatgca cttgctgtta tccgttgttc tgtatgcgcc ggggctgctg gttttcctgt
    13681 atgcgcgcaa aacgcataca catgataatg tactgaaccg tcaggaaatg gtgctgattg
    13741 gcatgctgtt aattgcctca gttccggcga cctggatgct ggtgggataa cgttcatacc
    13801 atcgttggat agcaaaggag ataacgatgg gtaaagccca gcccttgcca atattaatta
    13861 ctggcggagg tcgtcgcatc ggcctcgccc tcgcctggca tttcattaat caaaagcaac
    13921 cggtgattgt cagctatcgg acgcactatc cagccattga tggactgatt aaggcaggtg
    13981 cgcagtgtat tcaggctgat ttttcgacca acgacggtgt gatggcgttt gccgatgaag
    14041 tactaaaaag cacccatggt ctgcgtgcta ttttgcataa cgccagtgcg tggatggcgg
    14101 aaaaaccagg tacaccactg gccgacgtac tggcttgcat gatgcagatc cacgttaata
    14161 ccccatacct gctcaaccat gcgctggaaa gattactgcg tgggcacgga cacgccgcca
    14221 gcgatatcat tcactttacc gattatgtgg tggagcgcgg tagcgacaaa catgttgcgt
    14281 atgctgcaag caaagcggca ctggataata tgacccgctc gtttgcccgc aagctggcac
    14341 cggaagtgaa agtgaattct attgcgccat cgctgatcct gtttaatgaa catgatgatg
    14401 ccgaatatcg acaacaggcg ctgaataaat cactgatgaa aaccgcgcct ggcgagaaag
    14461 aagtgatcga cctggtcgat tacttactta ccagctgctt tgtcaccgga cgcagtttcc
    14521 cacttgatgg cggtcgtcat ctgcgttaat gcagctttat ccagcatata atcaacaccc
    14581 atgcgcttat cccccaacac gcaacagaac caacaaaagc ggcgggcaag cgcatcattc
    14641 cggtgaaata ccacagcgac accaggtaaa caaaataggg aataatcgac cacatactaa
    14701 aaatgatggt tgcgcgtaag gcttcaatgc cgcgttcgct ggcaacaata taatgcgcga
    14761 taagcgcaaa ggtcgggaaa agtggaatca gcccggcgat ataataattt ttcgtttttg
    14821 ctaaaacacc aatcaacagc actaccagcg caccgagcgc ggctttgatt accagcccca
    14881 tctttttacc ttaacacttc cataacaagt catcagtaga atacctgatg aaaacttgtt
    14941 tagaaacgat tgatagtaag taaaaacagc gcggtgtatt gtgacgtttt tatatctacc
    15001 gtgaatgtta tgaacactat cgtatttgtg gaagatgatg cggaagtcgg ttcactgatt
    15061 gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag aaccgcgcgg cgaccaggcc
    15121 gaagaaacca ttttgcgaga aagtccggat ttggtgttac tcgacatcat gctaccaggc
    15181 aaggacggca tgaccatttg tcgtgattta cgcgcaaagt ggtctggacc gattgttctt
    15241 ctaacctctc tcgatagcga tatgaaccac atcctggcac tggaaatggg tgcctgcgac
    15301 tatattctca aaacgacgcc ccctgctgtt ttgctagcgc gtttacgttt gcatttgcgt
    15361 cagaatgagc aagccacact gaccaaaggt cttcaggaaa cgtctctgac tccctacaaa
    15421 gccctgcatt tcggcacgtt gaccatcgat cccatcaacc gcgtagtcac cctggctaac
    15481 actgaaatct cgctctcgac agctgatttc gaattattgt gggaattagc tacccatgcc
    15541 gggcaaatca tggaccgcga tgcattgctg aaaaatttac gcggcgtcag ttatgacgga
    15601 ctggatcgta gcgtggacgt ggctatttcg cggttaagaa aaaaactgct cgataacgcc
    15661 gcagaacctt atcgcattaa aactgtgcgt aacaaaggct atctttttgc gcctcatgca
    15721 tgggaataag cgatgaaaaa actgtttatc cagttttacc tgttattgtt tgtctgcttc
    15781 cttgtgatgt ctctgctggt tgggttggtg tacaaattta ccgccgaacg cgcgggcaaa
    15841 cagtcgctgg atgatttgat gaacagttcg ctgtatctga tgcgcagcga attgcgtgag
    15901 atccccccac acgactgggg taagacgctg aaagagatgg atttaaatct ctctttcgat
    15961 ctgcgtgtcg agccactgag taaataccat cttgatgata tttccatgca ccgactgcgt
    16021 ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt tgcagcgtat cccgcgcagc
    16081 cactacgtgc tggcagttgg tcctgttcct tatctttatt atctccatca gatgcgattg
    16141 ctggatatcg ccctgatcgc ttttattgct atttccctcg cctttccggt gtttatctgg
    16201 atgcgtccgc actggcagga tatgttaaaa ctggaagcag cggcgcaacg atttggcgat
    16261 gggcatctca atgagcgtat ccactttgat gagggttcga gctttgaacg acttggtatc
    16321 gcatttaacc agatggcgga caatatcaac gccttgattg ccagcaaaaa acagcttatt
    16381 gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc tgcgttatcg actggagatg
    16441 agcgataacc tgagcgccgc cgaatcccag gcgttgaatc gtgatatcag tcaacttgaa
    16501 gctttaattg aagagctgct gacttatgcc cgactcgatc gcccacaaaa cgagcttcat
    16561 cttagcgaac cagacctgcc gttgtggctg tcaacgcatc tggcagatat tcaggcagta
    16621 acgcccgata aaacggtacg gataaaaacg ctcgtgcaag gccattatgc ggcgttggat
    16681 atgcgcttaa tggagcgcgt gctggataat ttgctcaata acgccctgcg gtactgccat
    16741 tcaacggttg aaaccagcct gctactgtcg gggaatagag cgacattaat tgttgaggat
    16801 gatggcccag ggattgcccc agaaaaccgc gaacatatct ttgaaccttt tgttcgcctc
    16861 gatcccagcc gggatcgctc aaccggcggc tgcgggctgg ggctggcaat tgtccactct
    16921 atagcactgg caatgggcgg tacggttaat tgtgacacca gcgaactggg tggtgctcgc
    16981 ttctcgttta gctggccgtt atggcataac atcccgcaat ttacctctgc ctgacactac
    17041 gcgcacgatg gtcaagtcac cacgactgtg ctataaaata agtatgttgt aactaaagtg
    17101 gttaatatta tggcgcgtta cgatctcgta gaccgactca acactacctt tcgccagatg
    17161 gaacaagagc tggctacatt tgccgctcat cttgagcaac acaagctatt ggttgcccgc
    17221 gtgttctctt tgccggaggt aaaaaaagag gatgagcata atccgcttaa tcgtattgag
    17281 gtaaaacaac atctcggcaa cgacgcgcag tcgctggcgt tgcgtcattt ccgccattta
    17341 tttattcaac aacagtccga aaatcgcagc agcaaggccg ctgtccgtct gcctggcgtg
    17401 ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg tcagtcatat tcagcacatc
    17461 aataaactca agaccacgtt cgagcatatc gtcacggttg aatcagaact ccccaccgcg
    17521 gcacgttttg aatgggtgca tcgtcatttg ccggggctga tcacccttaa tgcttaccgc
    17581 acgctcaccg ttctgcacga ccccgccact ttacgctttg gttgggctaa taaacatatc
    17641 attaagaatt tgcatcgtga tgaagtcctg gcacagctgg aaaaaagcct gaaatcacca
    17701 cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa aactggagcg agagtatcag
    17761 gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca aacgtccggt gaaggtgcag
    17821 ccgattgccc gcgtctggta caaaggagat caaaaacaag tccaacacgc ctgccctaca
    17881 ccactgattg cactgattaa tcgggataat ggcgcgggcg tgccggacgt tggtgagttg
    17941 ttaaattacg atgccgacaa tgtgcagcac cgttataaac cacaggcgca gccgcttcgt
    18001 ttgatcattc cacggctgca cctgtatgtt gcagattaac gcccggcttt catactgccg
    18061 accatctgtt ctggccgtac ccagctgtca aactcggctt cgctaagata ccccagcgca
    18121 agggccgcag cttttaaggt cagcccttct ttatgcgctt ttttggcgat ctccgcggct
    18181 ttgtcataac caatgtgggt gttaagcgca gtcaccagca tcagcgattc attgagtaat
    18241 tgattgattc gctcacgatt cggttcaata cccactgcgc agtgtttgtt aaaactttcc
    18301 atgccatctg ccagcaagcg caccgattgc aggaaattgt ggatcaccat tggacggaag
    18361 acgttcagtt caaagttacc ggaagcgccc cccatgttga tcgccacgtc gttccccatc
    18421 acctgacagc agagcatggt taatgcctca cactgtgttg gattcacttt ccccggcatg
    18481 attgagctgc ccggctcatt ttccgggatt gagatttcac caattccgca gcgcgggcca
    18541 gaggccagcc agcggacatc attggcgatt ttcatcagtg acacagccaa ccctttcaac
    18601 gcgccgtgcg cctgaaccag ggcatcacag gtcgccagcg cttcaaattt gttcggcgcg
    18661 gtaacaaacg gtgcacaggt aatgactgcc agttcatctg ctacgcgacg cgcatactcc
    18721 ggatgggtat ttagtccagt acccaccgct gtaccgccaa gagccagttc cgctacgtga
    18781 ggcaggctgt attcgatatg tttgagatta tgctcgagca tcgctaccca gccggaaatc
    18841 tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg ttcgaccgat tttgacgata
    18901 tcggcaaatg cacgcgattt ttcactcagt gtctgtgtca gggttttaag ctgcggaatg
    18961 agttgcttgc gcagcgccag cagcgccgca acgtgcatcg ccgtcggaaa gacatcgttg
    19021 gaactttggc ttttgttcac gtcgtcgtta gggtgaactt tacgttccat cccgcgcacg
    19081 ccgccgagta attcactggc ccggttagcc agcacttcgt tcatgttcat gttactttgc
    19141 gtgccggagc cggtctgcca gatagccagc gggaattcgt cgtcatgctg tcctgccagt
    19201 acttcatccg ccgcctgccg aatggcgctc gctttctctt cagacaacaa gcctaaatct
    19261 tcattaactt ttgccgctgc gcgcttggtt agcgccagcg catgaatcag tgaggtgggc
    19321 attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt gagtttgtgc gccccacagc
    19381 ttatctgccg ggacatcaat cgcccccatc gaatcttttt cgctgcgtac tgtattcatg
    19441 gcctgctcct cacctgttta attttttctt tctgttttgc tttcgttaag caacttttag
    19501 ctcacttatt atttactatt tgataacaaa tgtttggtct ttcgtgccat gtaaaaaaac
    19561 cgccccgaag ggcggctctg tttatttcac acagcgagtg cactgtgtga gttgtatctg
    19621 ctggaagaag tcatttcctt tatcatccac aaggataaac gccgggaaat cttccacttc
    19681 aattttccag atggcttcca ttcccagttc cggatattca acacattcca ggctcttaat
    19741 acttccctgc gccaatacag cggccggacc accgatactg ccaaggtaga agccgccgtg
    19801 ttttttacag gcatccgtca cctgctggct gcggttgcct ttcgccagca tgatcatact
    19861 tccgccctgc gcttgcagtt gatcgacata agaatccatc cgtccggcgg tcgttgggcc
    19921 aagagaaccg gaggcataac cttccggcgt tttggccgga cccgcgtagt aaatcggatg
    19981 atctttaatg tactgcggca gcccttcacc gttatccatc cgctctttca gtttggcgtg
    20041 agcaatatca cgaccgacga taatcgtgcc gttaagcgat aagcgtgtag aaacgggata
    20101 ctgcgacaac tgtgcgagga tctctttcat cggacggtta aggtcaacgc gcaccgcttc
    20161 gccttctccc gctttacgca gctcttcagg gatatattta cctggattat gttccagttt
    20221 ttcgatccag atcccctgac ggttgatctt cgctttgata ttacggtcag cagagcagga
    20281 aacacccata ccaaccggac aggatgcgcc gtgacgtggc aggcgaatca cgcgtatgtc
    20341 gtgagcgaag tatttaccac caaactgcgc acccagaccg agattttgcg cttcgatcag
    20401 caattctttt tccagttcca catcgcggaa tgcctgaccg tgctcattcc cttccgttgg
    20461 cagttcgtca tagtatttag cggaagccag tttcactgtt ttaaggttag tttctgcaga
    20521 agttccacca attacgaacg caatatgata cggaggacag gccgccgtac ccagcgtgcg
    20581 catcttctca accaggtaat ttttcagttt ccccggcgtt agtaacgctt tggtttcctg
    20641 atagagatac gtcttgtttg ccgaaccacc acctttggcg atacagagga atttgtactc
    20701 gtcgccatca acggcataaa gatcgatctg cgctggcaga ttggtgccgg tattcacctc
    20761 tttatacata tccagcggcg cgttttgcga gtagcgcaaa ttatcttcga tataagtgtt
    20821 atagacaccg cgcgccagcg ccgcttcatc acagccaccg gtccatacac gctgcccttt
    20881 tttaccaaca ataatcgcag tgccggtatc ctgacaggtt ggcagaacgc ctttcgccgc
    20941 gatgtcggag ttacgcagga attgcagcgc cacatattta tcattttcgc tggcctccgg
    21001 gtcacgcaga atgtcggcca cctgttgttg gtgagccgga cgcagcataa acgacgcatc
    21061 atgaaacgcc tgacgcgcca acagagttaa cgcttcgggt gcgactttca aaatctcctg
    21121 cccttcaaat tcagatacgc taacgtgttc gctggttagc aggtaatact cagtatcatc
    21181 ttttttgagt ggaaaaggag cctgataatg aaagggtttg tttgacattg ttctctcact
    21241 tactgcctgg tttggttatg ctctgggcgg gtgttccgtt gccctgttaa aagcgagtaa
    21301 caatatccta cacacttttt taacaaaaac tgagactagt acgacttttt gcggctccag
    21361 gttacttccc gtaggattct tgctttaata gtgggattaa tttccacatt aaaacaggga
    21421 ttgatcatgc aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg
    21481 ttgactgaac tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg
    21541 ggcgcacatc cgaaaagcag ttcacgagtg cagaatgccg ccggagatat cgtttcactg
    21601 cgtgatgtga ttgagagtga taaatcgact ctgctcggag aggccgttgc caaacgcttt
    21661 ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac agccgctctc cattcaggtt
    21721 catccaaaca aacgcaattc tgaaatcggt tttgccaaag aaaatgccgc aggtatcccg
    21781 atggatgccg ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg
    21841 ctgacgcctt tccttgcgat gaacgcgttt cgtgagtttt ccgagattgt ctccctactc
    21901 cagccagtcg caggtgcaca tccggcaatt gctcactttt tacaacagcc taatgccgaa
    21961 cgtttaagcg aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg
    22021 ctggcgattt taaaatcggc gctcgacagt cagcagggtg aaccgtggca aacgattcgt
    22081 ttaatttctg aattttaccc ggaagacagc ggcctgttct ccccgctgtt actgaatgtg
    22141 gtgaaattga atcctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg
    22201 caaggcgtgg cgctggaagt gatggcgaac tccgataacg tgctgcgtgc gggtctgacg
    22261 cctaagtaca ttgatattcc ggaactggtt gccaatgtga aattcgaagc caaaccggct
    22321 aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattccagtg
    22381 gatgattttg ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag
    22441 agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag
    22501 ttacagctta aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc
    22561 aaaggccacg gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt
    22621 aacatctctt gctaagctta ttaaaggctt ataacacctt caggcggcca gtccgcctga
    22681 tttcatttta tggataatca ttatgaataa atcgctggta gcggtaggcg tcattgttgc
    22741 gctaggcgta gtctggacag gcggcgcatg gtatacaggc aagaagattg aaacccatct
    22801 cgaagacatg gtcgcgcagg cgaacgcgca actcaaactg acagctcctg aatccaacct
    22861 ggaagtgagt tatcaaaact atcatcgcgg cgtattcagc agccagttgc aactgttggt
    22921 gaaacccatt gccgggaaag aaaatccgtg gattaaaagc ggtcagagcg tcatcttcaa
    22981 cgaatcggtt gatcatggtc ccttcccgct tgcccagctt aaaaaactga acctgatccc
    23041 gtcgatggca tcaattcaaa ccacgctggt taataacgaa gtaagcaaac cactgtttga
    23101 tatggcaaaa ggtgaaacgc cttttgagat taactcgcgc attggttaca gcggtgattc
    23161 cagttccgat atttcgctca agccactgaa ttacgagcaa aaggatgaaa aagtcgcctt
    23221 tagcggcggc gagttccagt taaatgctga cagagacggc aaagccatct ccctttccgg
    23281 ggaggcgcaa agtggtcgga tagacgcagt taacgaatac aaccagaaag tgcagttgac
    23341 ctttaataat ctgaaaaccg acggttccag cacgctggca agttttggtg agcgcgtagg
    23401 aaaccaaaaa ctgtcactgg aaaaaatgac catttcagtg gaaggcaaag aactggcact
    23461 gctggaaggc atggagatca gcggtaaatc ggatctggtc aatgacggta aaacgatcaa
    23521 tagccaactg gattactcgc taaacagcct gaaggtacag aatcaggatc tgggcagcgg
    23581 caagctgact ttaaaagtcg gccagattga tggtgaagcc tggcatcagt ttagccagca
    23641 atataacgcg caaactcagg cgctgctggc acagccagaa attgccaaca atcccgaact
    23701 ttatcaggag aaagtgacgg aagccttctt tagcgccctg ccgctgatgt tgaaaggcga
    23761 tccggtgatt actatcgcgc cgctaagctg gaaaaacagt cagggtgaaa gtgcgctgaa
    23821 tctgtcgctg ttcctgaaag atccggcaac gactaaagaa gcgccgcaaa cgctggcgca
    23881 ggaagtagat cgttcggtta aatctctgga tgcgaaactg accattccgg tggatatggc
    23941 aactgagttt atgactcagg tagcgaagct ggaaggttat caggaagatc aagcgaaaaa
    24001 actggcgaaa cagcaagttg aaggtgcatc agcaatgggg cagatgttcc gtctgaccac
    24061 cttgcaggac aataccatca ccaccagcct gcaatatact aacggtcaga taacgttaaa
    24121 cgggcagaaa atgccactgg aagatttcgt tggtatgttt gcaatgccgg cattaaatgt
    24181 tccggtcgta cccgctattc cgcagcagta attcacagac aaaacgccgg agatttttct
    24241 ctccggcgtt attttttact tcagcataaa gtcatactga atactcattt tcacatccca
    24301 gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt tcccgccctt tatcggcagc
    24361 aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc cgatagttca taccacctaa
    24421 gatacccgtg ccttcaatcc gcacatctcg tttactggat tcatagccgc gagtcacaat
    24481 cggtgcaaat gtcagcgtaa attgcggcgt taaacgatag ttgacgccca attgccagga
    24541 ctgcattcct tcacggttac gatcaagact taatgaaaag ggatagccaa tgtcggtatc
    24601 caccacggca ccattcattg catggcgcaa ataaccacca ctgccaaata cgcttcccca
    24661 cggcgcattc caggtcaatt gtccactaac caacgccgtt tcattgggct ggctggtatt
    24721 acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac tcccctttca acgtataatt
    24781 cgggtcgagc ggtatgcttt tctcgagaat actttgtatc tgattggaaa catctttctg
    24841 taccgatgcc cccagagaat agcgactggt atcatcatgc caactggcgg ctaaaaaaga
    24901 acgtgcgcga tgtttatcgt attttcccgt ctggctgtta tacacccctt catcacgaat
    24961 accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc cacattcccc acagatcaat
    25021 atttggtgtt aaagcccaat aacctaatgc tccttcagaa actgtcggta atgtacgttg
    25081 cgaactgcca gccattaacg gaatacgata aggtaagcta ccataactat aatccgtccc
    25141 gaagcgacct agtttaagca ggctattttc actgggtttt atttttactg cccccagggc
    25201 aaagccaaaa cttttatcac catcaagata ccaccgggta ctcatatcag cacgagcacc
    25261 taatttataa acataatatg ccccgccttc aacgccaata atattggaat aataaccact
    25321 attgaaatcg agcaatcctc cctgtaccca tgcgtaaata tctcttcccg cgccagcact
    25381 gggcttatcc gccctgcgca attcgttttt cagtctgatg cgtagtcccg cttcatcgtc
    25441 agccagttgc gccgcataag cagaggtaag gccagaggca gccgtcaggc aaataacggc
    25501 catggccact atttttctca tagttgcacc tttgatgttc tgattatttt tattgaatat
    25561 taattagtga tatcgctgat taattgctgc tgcacttttt tacgattatc aatttcaacc
    25621 acgatttctt tgaatttttt atccgtgagc ggataaaacc agataataac gaatgccagt
    25681 agcataaatc cgcaaggtac taaggcaatt gatgtgcgga tgcccataat aacttccggc
    25741 gtttgcacct gattggcgat atatccgctt aatcccaaaa taaaggcagg aattgaacct
    25801 ccgattgcct gaccgcattt acgggtaaat gagaatagtg aataggtgag cccttcaatt
    25861 cgcacgccgg tcaggtattc accgtattct acggtatcag cttccagtgc ccacatcaca
    25921 gtcatagtaa cgccctgacc aattgaagcg atggccaacg caaccaacgc caccggcagt
    25981 gaccagacgg aaacccagaa gaatagcaga taaccgcagg ttcccagcaa agcgccaatc
    26041 aggaaggtat tctttttacc gatcctcgcg accatccccg gcaccagcgg tgccgatgcc
    26101 acagtaccaa ccaggttttg caccagtacc agcacagtga acagcccggt atcatttaac
    26161 acatagcgca cgtagaacaa cgacgaggcg ctgaccgcga aggtcgaaat cagcacacac
    26221 agcgcaccga tgcacaacat aaacagcggg cgattccgtt tcagggtttg cagactgatc
    26281 ttcaatgacg gctgcgccac gatacgtacc acattctcac gcgtcgattt gaagcagatg
    26341 aagtaaagca ccattccggc aatcgccagc acaatcgtcc agaaatggta taccgacacc
    26401 atctcttccg ggctggagtt cttaatgctc ggtcctatca gaaatgccag gcagacaaag
    26461 gttaatgaag cggcaatccc gcgagccgcg cccagacggg cgcgggattg tggttgttgg
    26521 gtcatcgcgg tagcaagtga accataagga atattcacca ggctgtagca aagcccgagg
    26581 cccatgtagg tcaaatatgc atacaccact ttgctaccat ggctccagtc ggtcggcacc
    26641 cagaatacca gcacgctgaa gatcattaac ggcgcagtac cgaagagtaa aaacgggcgg
    26701 aattttcccc agcgggtatt cacactgtcc accactcgtc cggcaaagac gtcggcgaag
    26761 gcatcgaata cccgcaccag taacagcatg gtgcccgccg cagcggcacc gacgccagcg
    26821 acgtcggtgt agtaactcaa taggaagagc gcccccattg cgaaggcgaa gttattggcg
    26881 acgtcaccga ggctgtagcc gacgatggtg cgccaggaga gttgttgatt cattgtttgc
    26941 ctccctgctg cggtttttca ccgaagttca tgccagtcca gcgtttttgc agcagaaaag
    27001 ccgccgactt cggtttgcgg tcgcgagtga agatcccttt cttgttaccg ccaacgcgca
    27061 atatgccttg cgaggtcgca aaatcggcga aattccatac ctgttcaccg acgacggcgc
    27121 tgacgcgatc aaagacgcgg tgatacatat ccagccatgc acactgatac tcttcactcc
    27181 acatgtcggt gtacattgag tgcagcccgg ctaacgtatc cacgccgtat tcggtgatga
    27241 taatcggctg atgcagtttc tcctgccagg ccagaagttc tttttccagt accttctctg
    27301 ccgtttccaa atcgccgctt tggacatacc atccgtaata acggttcagg cacagcacat
    27361 caaagagatc gctgatggta tcggtgtgag cgtcgcagaa cattacattg acgcaggtga
    27421 tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag tggcgcgaaa tattcccgtg
    27481 caccttgcgg acgggtatcc ggttcgttgg caatactcca catcaccacg cttgggtggt
    27541 ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg cgcttgctga gtttccccgt
    27601 tgactgcctc ttcgctgtac agttctttcg gcttgttgcc cgcttcgaaa ccaatgccta
    27661 aagagaggtt aaagccgaca gcagcagttt catcaatcac cacgatgcca tgttcatctg
    27721 cccagtcgag catctcttca gcgtaagggt aatgcgaggt acggtaggag ttggccccaa
    27781 tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt atcgaatcct ttgccacgca
    27841 agtccgcatc ttcatgacga ccaaagccag taaagtagaa cggtttgtgg ttaatcagga
    27901 actgttcgcc cttcactgcc actgaccgga tgccgacgcg aagcgggtag atatcacact
    27961 ctgtctggct tttggctgtg acgcacagtt catagagata accttcaccc ggttgccaga
    28021 ggtgcggatt caccacttgc aaagtcccgc tagtgccttg tccagttgca accacctgtt
    28081 gctccgcatc acgcagttca acgctgacat caccattggc caccacctgc cagtcaacag
    28141 acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt gatatcgtcc acccaggtgt
    28201 tcggcgtggt gtagagcatt acgctgcgat ggatcccggc atagttaaag aaatcatgga
    28261 agtaagactg ctttttcttg ccgttttcgt cggtaatcac cattcccggc gggatagtct
    28321 gccagttcag ttcgttgttc acacaaacgg tgatacgtac acttttcccg gcaataacat
    28381 acggcgtgac atcggcttca aatggcgtat agccgccctg atgctccatc acttcctgat
    28441 tattgaccca cactttgccg taatgagtga ccgcatcgaa acgcagcacg atacgctggc
    28501 ctgcccaacc tttcggtata aagacttcgc gctgatacca gacgttgccc gcataattac
    28561 gaatatctgc atcggcgaac tgatcgttaa aactgcctgg cacagcaatt gcccggcttt
    28621 cttgtaacgc gctttcccac caacgctgat caattccaca gttttcgcga tccagactga
    28681 atgcccacag gccgtcgagt tttttgattt cacgggttgg ggtttctaca ggacgtaaca
    28741 taagggactc ctcattaaga taaaaatact ggtcaacctt taatctgatt agttaaccaa
    28801 ttctgtgttg cacacagtta tctgtgagaa acccatggaa gagaaatgga aataaaaaga
    28861 actgacaccg cgatcaaaaa accaaaaact gcaataccca cagcgtgata ttgatcgcat
    28921 taatctttaa aacggcgttg acagaagcca taaaataagc aaccgaacgt tcgttcgagc
    28981 atattgggaa cgataaactc tcatgacata tggctacggt gaatattttt gggggggaat
    29041 tctccgatcc cggctagatt gtattttgta cagggaaatt aaagtatgaa acgcgggcac
    29101 agttatcacc agcccgcgaa atgagaggat caggatgcgg ttaagatacc gccaatcatc
    29161 gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat cttccgcttc gatatcggcc
    29221 agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca ttgacgtgtt gatgtctggg
    29281 cttatctcgc ctttttgctg ggcatcacgc atccgctcgg caacaaattc agtgatcgtc
    29341 atatgcttat ttttaagcat ggcggcgacc tgtgggttac gcatcccttc cgccataatt
    29401 tcgaccacca gcgcccgttg cccaaaggct tcatgggtga gagagacaat agactcgacc
    29461 atatagtcaa caaaatgaat cccttcaatc ggttcccgga aacgggccag cgccctctcc
    29521 tggtcctgta agataatcgc ctgaatcaag gcttctttgg agatgaaatg gtgatagagc
    29581 gtcccgggac taatggcgca ggatttacag atggctttca tcgaggcact gtgaaatcca
    29641 ttttctgaaa aaatctctct ggcagcattg aggatccggg tccgtgtcgg ttgtgcttca
    29701 gtctgcatgt tatccatcat aaaaccatta tagttagcgt gaataactag tttaccgcgt
    29761 tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa ggcacactct attcgtatac
    29821 ttttcactct gaaattggtt aaccaatagc cgtgatttgg ctgccagtta tttagcagag
    29881 acgttgatga gtttatcccg gtaaacaaca cgcacggttg tttaccggtc cgttagtgta
    29941 ttaattgagc tcctgtaccc caccaccgga gacggtgaga atttgtccgc ttacccagct
    30001 cgcagcaggc gagcaaagga acagcgctgc gttagcaata tcttgcggtt ggcccagacg
    30061 tctgatcggc gtgtgctgta acattttttg ttcaatttct ggtgtaataa cggatttcag
    30121 ggcatcggtt aatattgccc ccggcgcaat gccatttacc cgaatatttt tttcacccag
    30181 gtcaaacgcc atatttctga ccagatgact ggctgcagct ttagatgatg cataggaagt
    30241 catgtttata tttttatttt ctgccgccat agaagtgatg gtcagaataa cgccaccgcc
    30301 atttttttcc atttctggcg caacaagttg tgacagatgg aaaaaagaaa acacattcag
    30361 ttcataagca cggcgaaaat ccgccattgg catatcaaac ggtttaggtc caccgccacc
    30421 ggcgttgtta accagaatat caactttacc cagcttactg atagcaaagt ctgccagtgc
    30481 agagagttcc tgttcggaag taatatcaca acggcaggca aatgcctgac cacccagttg
    30541 ttgaatttcg tctacaacat ggttagctgc gtcggcgtta atatcactga ccaccacaga
    30601 tgcgccagct gtcgcgaatg taatggcgat ttctttacca atacctgcac ccgcacctgt
    30661 gatgatggcg cattttccgt cgagtctcag gttgtcagaa ttaaacacat aaacctcctg
    30721 tgaacttcag taaaaataaa aggcaccgcc tgtgtgccac attttttatc attaaccttg
    30781 aggaaaggat actaccaggt atagcagata aagagaggat tatttcgccg caatgagccg
    30841 ggcgggaata ataagattgc gtgaatgcgt ctcttcatgg gtgatttttt gcatcatgcg
    30901 atccgcaagt gtgataccaa gttcccgcgc tggcgtgctg gcccaggtaa caggtatatc
    30961 atcaagtgtg gttggtgtcg catcggtaaa tgccgccagc gaaacctgtt gctcaaagta
    31021 acgatcgact ccgctttccc cgctttgccg ccctgctttg agcaaaccaa accatgcccc
    31081 catcgcaata gtttcgttat agcaaaccac ggcactgatg gtcgggttat gacgtaaaag
    31141 cgccgtgata gcttccgcgg cttgcttctg gctggaagtg cactccaaca cccaatcgct
    31201 atgaaacggc aggccaaatt ttagtagagt tgcacaatag ccccccaccc gttctgcacg
    31261 ggttaatgag gaactttgcc ctcccagcca ggcgatccgc tgatgcccat tgcgaatgag
    31321 atgctccgtc aacaactgtg cagcctgcat gttgtccggg cgaaccgtat caacatcatc
    31381 aagataactg gcacgggaag cgaaaatcac cgggatagct ttttcttctg ccatccgtcg
    31441 caggtcatcg ctgcttcctg cagccccggc aattaccaca ccatccacac cctgattcag
    31501 taacagtgaa aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac
    31561 catccgtccc tgcgcttcca gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg
    31621 cgcagataaa tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga
    31681 cgcctggcga ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt
    31741 ggcggtagag attcgccctt tgccactaag caccagcgaa acggtactta ccgacacgcc
    31801 cgcagccagc gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta
    31861 ctccctgatt atgtgacaga taaaacgttt taccttttat ttatcttata ctcgctatta
    31921 tcgttgcgta atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg
    31981 caatttactg aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca
    32041 ccgaaagtca cgctgtggga gttcttccag cagttaggca aaaccttcat gttacccgtg
    32101 gcattattgt cgttctgcgg cattatgctc ggcattggta gttctcttag tagccacgat
    32161 gtcatcaccc tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg
    32221 agtaagattg gctcgtttgc ttttagtttc ctgccagtca tgttctgtat cgccatcccg
    32281 ctgggtctgg cacgcgaaaa caaaggcgta gcggcattcg ctggcttcgt cggttatgcg
    32341 gtaatgaacc tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggac
    32401 gccgcggttc tgaaagccaa taacatccag agcattcttg ggatccagtc gatcgacacc
    32461 ggtatcctcg gtgcggtgat cgccggtatt atcgtctgga tgctgcatga gcgattccat
    32521 aatatccgcc tgccggatgc gcttgcattc ttcggcggta cgcgcttcgt accaatcatc
    32581 tcctcgctgg tgatgggcct tgtcggcctg gtgattccat tagtctggcc gattttcgcg
    32641 atgggtatta gcggcttggg ccatatgata aacagcgcgg gtgatttcgg accgatgctg
    32701 tttggtaccg gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta
    32761 attcgcttta ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca
    32821 ttgaccatct tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc
    32881 acgcgtttcc tttcgcaagg taaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg
    32941 ttagctatgt atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt
    33001 tctggcctga tcgcctgcgt cgttggcggc actaccgaac cgctggaatt cctgttcctg
    33061 ttcgtagcgc cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc
    33121 atgtctgtgc tcggcgtcac catcggtaat accgacggca atatcatcga cttcgtggtg
    33181 ttcggtattt tgcatggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc
    33241 tggtttgtcg tttactacgt catcttccgt ttcgctatca cccgcttcaa tctgaaaacc
    33301 ccggggcgcg atagcgaagt tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt
    33361 aaatcaggtt acaacgttcc tgcaatcctc gaagcattag gcggtgccga caatattgtc
    33421 agcctcgata actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat
    33481 gtgctggcac tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg
    33541 caggttgtta tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat
    33601 actgtccagg cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc
    33661 acatggtgta cacagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg
    33721 ttcacgattt cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag
    33781 cgcctgatgc acggcgtatt tggctacagc cgctggaaaa acgatgagtt tctcgcggct
    33841 attgcccact ggttttccac ccagcattac accgccatcg attctcagac ggtggtgtat
    33901 ggcccttctg tcatctatat ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa
    33961 ggcgtggtga tccacacacc cgcctatgac gcattttaca aggccattga aggtaaccag
    34021 cgcacagtaa tgcccgttgc tttagagaag caggctgatg gttggttttg cgatatgggc
    34081 aagttggaag ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag
    34141 aatcctaccg ggaaagtgtg gacgtgcgat gagctggaga tcatggctga cctatgcgag
    34201 cgtcatggtg tgcgggttat ttccgatgaa atccatatgg atatggtctg gggcgagcag
    34261 ccgcatattc cctggagtaa tgtggcgcgg ggagactggg cgttgctgac gtcgggctcg
    34321 aaaagtttca atattcccgc gctgaccggt gcttacggga ttatagaaaa tagcagcagc
    34381 cgtgatgcct atttatcggc actgaaagga cgtgatgggc tttcttcccc ttcggtactg
    34441 gcgttaactg cccatatcgc cgcctatcag caaggtgcac cgtggctgga tgccttacgc
    34501 gtctatctga aagataacct gacgtatatc tcagataaaa tgaacgccgc gtttcctgaa
    34561 ctcaactggc agatcccgca atccacttat ctggcctggc ttgatctacg tctgttgaat
    34621 attgacgaca acgcgttgca aaaagcgctt atcgagcaag aaaaagtcgc gatcatgccg
    34681 gggtatacct atggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt
    34741 tcgaaactgg aaaaaggtgt ggctggatta ataaacgcca tccgcgctgt tcgctaaccc
    34801 caattgcgca acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc tttgttttta
    34861 taatggtgcg cacttttata ttcagaaaaa gagtgcgacc atgattgata ccaccctgcc
    34921 attaactgat atccatcgcc accttgatgg caacattcgt ccccagacca ttcttgaact
    34981 tggccgccag tataatatct cgcttcctgc acaatccctg gaaacactga ttccccacgt
    35041 tcaggtcatt gccaacgaac ccgatctggt gagctttctg accaaacttg actggggcgt
    35101 taaagttctc gcctctcttg atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc
    35161 agcccgtcac ggcctgcact atgtcgagct gcgtttttca ccaggctaca tggcaatggc
    35221 acatcagctg cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg
    35281 ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg agccggacct tcggcgaagc
    35341 cgcctgtcag caagagctgg aggccttttt agcccaccgt gaccagatta ccgcacttga
    35401 tttagccggt gatgaacttg gtttcccggg aagtctgttc ctttctcact tcaaccgcgc
    35461 gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat
    35521 ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga catggcgtaa aagccattga
    35581 agatcgggcg ctgatggatt ttctcgccga gcaacaaatt ggtattgaat cctgtctgac
    35641 ctccaatatt cagaccagca ccgtagcaga gctggctgca catccgctga aaacgttcct
    35701 tgagcatggc attcgtgcca gcattaacac tgacgatccc ggcgtacagg gagtggatat
    35761 cattcacgaa tataccgttg ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca
    35821 ggcacagatt aatggtctgg aaatggcttt cctcagcgct gaggaaaaac gcgcactgcg
    35881 agaaaaagtc gccgcgaagt aacaaaatgg atggtgcaaa ttgcaccatc catttttcat
    35941 gcaaggcaca aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat caactccatt
    36001 acctggattg cctggcttgc cggaaccgga ttttcaccat cgccatttaa cgcatcacga
    36061 atagccgcat agtaagccgg ataattccca ggtaccgtca acagcgtttc ttcgacacgt
    36121 tcctcacctt ccacgcgggt aagtacgcca tcacgcatat cgtagcccca gtcttcctgc
    36181 ggcagacgct cgccattttt cagacgttct tcctgtggat cgaggccata tttcacataa
    36241 ctgcctcggg atccatgcac gatataccgt gctgactcag cggctgccag catggtaccg
    36301 tgtaaaatga ctcgccgctg tggataggac aagatggcgt ggaaataatc ggtcgactgc
    36361 gctccgggcc gtaactgtgc caaatctacc gtcatgctga ccggtaaacc aaaaagcgta
    36421 atggcctgat caagaagatg tggtgctaaa tcgtaccaga taccgctgcc tgggccgccc
    36481 tgttcacgcc aacgatcgcg cacctgcgga cggaagcggt caaaatgaga ctcaaagtaa
    36541 gcaacttcac ccagcacgcc ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc
    36601 cagcgacggt tatggaatac agacagcaca cgccccaggc tttttgccag cgcatccagc
    36661 tctcgcgctt gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg tttacccgcc
    36721 tcaagcgccg ctttggctaa cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg
    36781 tctatgttgg gatcgttaaa cagatgcttc ggctcagaga caaccgtaac cgttggccag
    36841 tcggctttta cttttgtttc atcactgctg gagattaccg ccagttccag cccgggcgtg
    36901 cccgcaatca ggggcgcatg gaaggttttg ctcgcataac catacccaat caacccaaca
    36961 cggatgttgt cgctcatgtc attgcctctc attgaagtat gatggctatt tgacactatc
    37021 ctttacccac gctcaacagt ttaataacct gccagcaata agggatgttg tttaacttaa
    37081 gtcaaaaaaa tagcgaattt tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa
    37141 aatcttgctg gagctattat tgctaagtaa catttacccc ctgaagttaa tggatcaatc
    37201 aagagagatg tgggctgtaa tgaatcgtct tattgaatta acaggttgga tcgttcttgt
    37261 cgtttcagtc attcttcttg gcgtggcgag tcacattgac aactatcagc cacctgaaca
    37321 gagtgcttcg gtacaacaca agtaagctct gcacttgtgg agcgacatgc tgcccgtccg
    37381 ggtgcatgtt ttcacttgtc ggatattaaa ccaggaattt attatcttgt tcgatgttgt
    37441 tggtgattgt cagggatagt aaagcgcgtt actcttttca gagcagtttt tcatccctga
    37501 atatttgcca tcagcgcatt cttattattg taataacaca agcgttcact tttggtgaaa
    37561 ttttatcatc actaataata cctacccctt tggtattaat tattttatgg accttttatg
    37621 actgttcagg actacttatt aaaatttcgc aaaatcagtt cactcgaaag tctggaaaaa
    37681 ctctacgacc atcttaatta caccctgacg gacgatcagg aactgatcaa tatgtatcgt
    37741 gctgccgatc accgtcgcgc agagctggtt tctggcgggc gtttgtttga cctcggccag
    37801 gtaccgaagt ccgtctggca ctatgtccaa taaagaaagt agcgatcggt tataaaagcc
    37861 ttataataac gcccctaaaa ttagatttgc ctgtttgcgt cgctattctg gagaacctat
    37921 gaccaccaca acgccacaac gtattggagg ctggttgctt ggcccgttgg cctggctttt
    37981 agtcgctctg ttgagtacga cgctggcgtt gttgctgtat accgctgcgt tatcttctcc
    38041 gcaaacattt caaacgctcg gcggacaagc actgacgaca caaatcttat ggggcgtttc
    38101 ttttattacc gctatcgcca tgtggtatta cacactgtgg ttgaccatcg ctttctttaa
    38161 acgtcggcgt tgtgttccta aacactatat tatctggctg cttatttccg tattgctggc
    38221 agtaaaagca ttcgcttttt cacccgtgga agatggcata gccgttcgcc agttgctgtt
    38281 tactttgttg gcaaccgcat tgatcgtccc ctatttcaaa cgctcgtcgc gggtaaaagc
    38341 gacgtttgtg aatccgtaat aaccttacag ttaacctgtt gtcgcctgct ctggattaac
    38401 ggataatagg cggctttttt atttcaggcc gaaaaatgac tgactacctg ttactgtttg
    38461 tcggaactgt actggtcaat aactttgtac tggtcaagtt tctcggtctc tgtccgttta
    38521 tgggggtttc caaaaaactg gaaaccgcga tgggcatggg gctggcaaca acgtttgtga
    38581 tgacgctggc gtctatttgc gcctggctta tcgatacgtg gattttgatc ccacttaatc
    38641 tgatttacct gcgcaccctg gcatttattc tggtgattgc tgtggtcgtg cagttcaccg
    38701 agatggtggt gcgcaaaacc agcccggtgc tttaccgtct gctggggatt tttttgccgc
    38761 ttatcaccac caactgtgca gtgctcggcg tggcgttgct gaatatcaat ctcgggcaca
    38821 atttcttgca gtcggcgctg tacggttttt ccgccgctat cggtttctcg ctggtgatgg
    38881 tgctcttcgc cgccatccgc gaacgccttg ctgtggctga tgtcccggca ccttttcgcg
    38941 gtcatgccat tgcgttaatt accgcaggtc ttatgtctct ggcctttatg ggctttagtg
    39001 gtttggtgaa gttgtaatga atgctatctg gattgccgtt gccgccgtga gcctgctggg
    39061 cctggcgttt ggcgccattc tgggttatgc ctcccgccgt tttgcggtgg aagacgatcc
    39121 ggtcgttgag aaaattgacg aaatcttacc gcagagccag tgtggtcagt gcggttatcc
    39181 cggctgtcgc ccctacgcgg aagccatcag ctgtaacggt gaaaaaatca accgttgcgc
    39241 cccaggtggc gaagctgtga tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc
    39301 gctggatggc gaagcgcaag agataacgcc tgcgcggatg gtggcggtta ttgatgaaaa
    39361 taactgtatt ggctgcacta aatgtattca ggcgtgtccg gtagacgcca tcgttggcgc
    39421 tacccgtgcc atgcatacgg taatgagtga tctctgtacg ggctgcaatt tatgtgttga
    39481 tccgtgcccg acgcactgca tctcgttgca accggtcgca gaaacacctg actcctggaa
    39541 atgggatctg aacaccattc ccgtgcgtat cattcccgtg gaacaccatg cttaagttat
    39601 tctctgcatt cagaaaaaat aaaatctggg atttcaacgg cggcatccat ccaccggaga
    39661 tgaaaaccca gtccaacggt acacccctgc gccaggtacc actggcgcag cgttttgtta
    39721 ttccactgaa acagcatatt ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag
    39781 tattgcgcgg ccagccgctt acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca
    39841 cctcgggtac cgttacggct attgcgcccc actctacggc tcatccttca gctttagctg
    39901 aattaagcgt gattattgat gccgatggtg aagactgctg gatcccgcgc gacggctggg
    39961 ccgattatcg cactcgcagt cgcgaagagt taatcgagcg catacatcag tttggtgttg
    40021 ccgggctggg cggtgcagga ttcccgacag gcgttaaatt gcagggtggc ggagataaga
    40081 ttgaaacgtt gattatcaac gcggctgagt gcgagccgta cattaccgcc gatgaccgtt
    40141 tgatgcagga ttgcgcggct caggtcgtag agggtattcg cattcttgcg catattctgc
    40201 agccacgcga aattcttatc ggcattgaag ataacaaacc gcaggcgatt tccatgctgc
    40261 gcgcggtgct ggcggactct aacgatattt ctctgcgggt gattccaacc aaatatcctt
    40321 ctggcggtgc taaacaatta acctacattc tgaccgggaa gcaggttcca catggcgggc
    40381 gttcatccga tatcggcgta ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg
    40441 ccgttattga tggcgagccg attaccgagc gtgttgtaac cctgactggc gaagcaatcg
    40501 ctcgcccggg caacgtctgg gcacggctgg ggacgccagt gcgtcattta ttgaatgatg
    40561 ccggattctg cccctctgcc gatcaaatgg tgattatggg tggcccgcta atgggcttta
    40621 ccttgccatg gctggatgtc ccggtcgtaa agattaccaa ctgtctgttg gctccctctg
    40681 ccaatgaact tggcgaacca caggaagaac aaagctgcat ccggtgtagc gcctgtgctg
    40741 acgcctgccc tgcggatctt ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac
    40801 acgataaagc taccacgcat aacattgctg attgcattga atgtggggct tgcgcgtggg
    40861 tttgcccgag caatattccc ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg
    40921 ctattcgtca ggaagaaaag cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg
    40981 ctcgtctgga gcgcgaaaaa gcggctcgcc ttgaacgaca taagagcgca gccgttcaac
    41041 ctgcagccaa agataaagat gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg
    41101 cccaggctac acagcctatt gtgattaaag cgggcgaacg cccggataac agtgcaatta
    41161 ttgcagcacg ggaagcccgt aaagcgcaag ccagagcgaa acaggcagaa ctgcagcaaa
    41221 ctaacgacgc agcaaccgtt gctgatccac gtaaaactgc cgttgaagca gctatcgccc
    41281 gcgccaaagc gcgcaagctg gaacagcaac aggctaatgc ggaaccaga
//
LOCUS       DAZFKM010000028        39453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFKM010000028 DAZFKM010000000
VERSION     DAZFKM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            246..665
                     /gene="umuD"
                     /locus_tag="ACHKAY_001629"
     CDS             246..665
                     /gene="umuD"
                     /locus_tag="ACHKAY_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO5700324.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            665..1932
                     /gene="umuC"
                     /locus_tag="ACHKAY_001630"
                     /pseudo
     CDS             665..1932
                     /gene="umuC"
                     /locus_tag="ACHKAY_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(1978..2508)
                     /gene="dsbB"
                     /locus_tag="ACHKAY_001631"
     CDS             complement(1978..2508)
                     /gene="dsbB"
                     /locus_tag="ACHKAY_001631"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO5700325.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2654..4195)
                     /gene="nhaB"
                     /locus_tag="ACHKAY_001632"
     CDS             complement(2654..4195)
                     /gene="nhaB"
                     /locus_tag="ACHKAY_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO5700326.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4417..5136
                     /gene="fadR"
                     /locus_tag="ACHKAY_001633"
     CDS             4417..5136
                     /gene="fadR"
                     /locus_tag="ACHKAY_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO5700327.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5188..6720)
                     /gene="ycgB"
                     /locus_tag="ACHKAY_001634"
     CDS             complement(5188..6720)
                     /gene="ycgB"
                     /locus_tag="ACHKAY_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO5700328.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7050..8348
                     /gene="dadA"
                     /locus_tag="ACHKAY_001635"
     CDS             7050..8348
                     /gene="dadA"
                     /locus_tag="ACHKAY_001635"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO5700329.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8358..9428
                     /gene="dadX"
                     /locus_tag="ACHKAY_001636"
     CDS             8358..9428
                     /gene="dadX"
                     /locus_tag="ACHKAY_001636"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO5700330.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9814..11550)
                     /gene="cvrA"
                     /locus_tag="ACHKAY_001637"
     CDS             complement(9814..11550)
                     /gene="cvrA"
                     /locus_tag="ACHKAY_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO5700331.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11645..12559)
                     /gene="ldcA"
                     /locus_tag="ACHKAY_001638"
     CDS             complement(11645..12559)
                     /gene="ldcA"
                     /locus_tag="ACHKAY_001638"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO5700332.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12659..13270
                     /gene="emtA"
                     /locus_tag="ACHKAY_001639"
     CDS             12659..13270
                     /gene="emtA"
                     /locus_tag="ACHKAY_001639"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO5700333.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13272..14006)
                     /gene="ycgR"
                     /locus_tag="ACHKAY_001640"
     CDS             complement(13272..14006)
                     /gene="ycgR"
                     /locus_tag="ACHKAY_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO5700334.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14207..14461
                     /gene="ymgE"
                     /locus_tag="ACHKAY_001641"
     CDS             14207..14461
                     /gene="ymgE"
                     /locus_tag="ACHKAY_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO5700335.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14639..15079
                     /gene="ycgY"
                     /locus_tag="ACHKAY_001642"
     CDS             14639..15079
                     /gene="ycgY"
                     /locus_tag="ACHKAY_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO5700336.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15158..16855)
                     /gene="treA"
                     /locus_tag="ACHKAY_001643"
     CDS             complement(15158..16855)
                     /gene="treA"
                     /locus_tag="ACHKAY_001643"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5700337.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17175..18593)
                     /gene="dhaM"
                     /locus_tag="ACHKAY_001644"
     CDS             complement(17175..18593)
                     /gene="dhaM"
                     /locus_tag="ACHKAY_001644"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO5700338.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18604..19236)
                     /gene="dhaL"
                     /locus_tag="ACHKAY_001645"
     CDS             complement(18604..19236)
                     /gene="dhaL"
                     /locus_tag="ACHKAY_001645"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO5700339.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19247..20317)
                     /gene="dhaK"
                     /locus_tag="ACHKAY_001646"
     CDS             complement(19247..20317)
                     /gene="dhaK"
                     /locus_tag="ACHKAY_001646"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO5700340.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20545..22464
                     /gene="dhaR"
                     /locus_tag="ACHKAY_001647"
     CDS             20545..22464
                     /gene="dhaR"
                     /locus_tag="ACHKAY_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO5700341.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22564..25449)
                     /gene="ycgV"
                     /locus_tag="ACHKAY_001648"
     CDS             complement(22564..25449)
                     /gene="ycgV"
                     /locus_tag="ACHKAY_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5700342.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26290..27381)
                     /gene="ychF"
                     /locus_tag="ACHKAY_001649"
     CDS             complement(26290..27381)
                     /gene="ychF"
                     /locus_tag="ACHKAY_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO5700343.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27498..28082)
                     /gene="pth"
                     /locus_tag="ACHKAY_001650"
     CDS             complement(27498..28082)
                     /gene="pth"
                     /locus_tag="ACHKAY_001650"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO5700344.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28360..28638
                     /gene="ychH"
                     /locus_tag="ACHKAY_001651"
     CDS             28360..28638
                     /gene="ychH"
                     /locus_tag="ACHKAY_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO5700345.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28693..30372)
                     /gene="dauA"
                     /locus_tag="ACHKAY_001652"
     CDS             complement(28693..30372)
                     /gene="dauA"
                     /locus_tag="ACHKAY_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO5700346.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30497..31444)
                     /gene="prs"
                     /locus_tag="ACHKAY_001653"
     CDS             complement(30497..31444)
                     /gene="prs"
                     /locus_tag="ACHKAY_001653"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO5700347.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31595..32446)
                     /gene="ispE"
                     /locus_tag="ACHKAY_001654"
     CDS             complement(31595..32446)
                     /gene="ispE"
                     /locus_tag="ACHKAY_001654"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO5700348.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32446..33069)
                     /gene="lolB"
                     /locus_tag="ACHKAY_001655"
     CDS             complement(32446..33069)
                     /gene="lolB"
                     /locus_tag="ACHKAY_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO5700349.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33283..34539
                     /gene="hemA"
                     /locus_tag="ACHKAY_001656"
     CDS             33283..34539
                     /gene="hemA"
                     /locus_tag="ACHKAY_001656"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO5700350.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34581..35663
                     /gene="prfA"
                     /locus_tag="ACHKAY_001657"
     CDS             34581..35663
                     /gene="prfA"
                     /locus_tag="ACHKAY_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO5700351.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35663..36496
                     /gene="prmC"
                     /locus_tag="ACHKAY_001658"
     CDS             35663..36496
                     /gene="prmC"
                     /locus_tag="ACHKAY_001658"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO5700352.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36493..36885
                     /gene="sirB2"
                     /locus_tag="ACHKAY_001659"
     CDS             36493..36885
                     /gene="sirB2"
                     /locus_tag="ACHKAY_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO5700353.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36889..37698
                     /gene="sirB1"
                     /locus_tag="ACHKAY_001660"
     CDS             36889..37698
                     /gene="sirB1"
                     /locus_tag="ACHKAY_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO5700354.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37734..38588
                     /gene="kdsA"
                     /locus_tag="ACHKAY_001661"
     CDS             37734..38588
                     /gene="kdsA"
                     /locus_tag="ACHKAY_001661"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO5700355.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38737..38844)
                     /gene="ldrA"
                     /locus_tag="ACHKAY_001662"
     CDS             complement(38737..38844)
                     /gene="ldrA"
                     /locus_tag="ACHKAY_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5700356.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39272..39379)
                     /locus_tag="ACHKAY_001663"
     CDS             complement(39272..39379)
                     /locus_tag="ACHKAY_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5700357.1"
                     /translation="MTLAQFAMIFWHDLAASILAGIITAAIVGWWRNRK"
BASE COUNT         9781 a         9842 c        10157 g         9673 t
ORIGIN      
        1 tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc tattttttag
       61 ccgggtaata tttttcattt ctgctggatg agcgtagtcg ccagaaggcc gcgtgagcac
      121 aggataagag aacgaaaaat cagcagtcta tgcagcgaca aatattgata gcctgaatca
      181 gtattgatct gctggcaaga acagactact gtatataaaa acagtatagc ttcaggcaga
      241 ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta
      301 gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgttgaa cagcgcatcg
      361 atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg
      421 attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta
      481 ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat
      541 tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg cccattacca
      601 tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc
      661 gctgatgttt gctctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg
      721 ccctgattta tggggtaaac cggtggttgt gctatcgaat aatgacggtt gcgttatcgc
      781 ccgaaacgct gaggcaaagg cgctgggtgt taaaatgggc gatccctggt tcaaacaaaa
      841 agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc tttacgcaga
      901 catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag
      961 tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc tgactgattt
     1021 tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat
     1081 cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca
     1141 gacggggggg tggtggattt atcaaatctg gaacgccagc gtaaattaat gtctgctctc
     1201 cccgtggatg aagtctgggg gattggacgg cggatcagca aaaaactgga cgcgatgggg
     1261 atcaaaaccg ttcttgattt ggcggataca gatatccggt ttatccgtaa acattttaat
     1321 gtcgtgctcg aaagaacggt gcgtgaactg cgcggcgaac cctgtttgca actggaagag
     1381 tttgcaccga cgaagcagga aattatctgt tcccgctcgt ttggtgaacg catcacggat
     1441 tatccatcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga aaaacttcgc
     1501 agcgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc atttgcgctc
     1561 aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac tcaggacagc
     1621 agggatatca ttaacgctgc tacgcgatct ctggatgcca tctggcaagc gggccatcgc
     1681 taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc gcagctcaat
     1741 ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatgacggt aatggataca
     1801 ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat ccagcaacaa
     1861 tggcagatga agcgtgccat gctttcacca cgttatacaa cgcgaagttc tgatttactg
     1921 cgggtcaaat aaatatagcg gcaggaaaaa agcgctcccg caggagcgcc gaatggatta
     1981 gcgaccgaac aggtcacgtt ttttcgcttt aaacggctgg gaaatcacca ccagcaccgc
     2041 gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca tacctaaaaa
     2101 atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca cccatttatc
     2161 cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaacg gcgaaggata
     2221 gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac tatacaacca
     2281 gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa tcagcgcagc
     2341 acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag gtttcagtaa
     2401 catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa acgccatcaa
     2461 caaccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa tcatttccct
     2521 gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc aggaggcagc
     2581 aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca tttatatgcc
     2641 cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat tcggttacag
     2701 gggcaagcgt aaactcgacg cagagcaagc cgacgagtgt cagtacgagg gtgtaaggca
     2761 gggccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt gcagaggtca
     2821 gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac ggagggcaga ttggtaccgg
     2881 tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca ctttccattg
     2941 ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa atggatgaca
     3001 gcagaccgtt gaaaatatag aacagcgaca gctgagcatg ttccgatgcc tgcaacacaa
     3061 actgaataat tggcgaaaac agttgttggt cgataatcac cgcgactacc gagaaaaaca
     3121 ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata gcatgttcat
     3181 cggtgacacc ggtcaatgat gtcgccagaa taatgactga caaaccaatc aagccaactt
     3241 ccgccagatg caacgccagc gcagtcacca gccagacgcc aataatcgcc tggacaatca
     3301 gacggatttt atcctgacga gtacgctggt ggcggctttg atcgtcaaac tgttgcaaca
     3361 cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc ttctctacca
     3421 gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac atgcgcagga
     3481 agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt ggttcgccca
     3541 ccatggtcat cacgccgcct aatgcagtgc cgacaccggc atgcatcatc aggctacgca
     3601 gaaagccacg gaactgttcc agaaccactt tgtaatgctt gtcgatatga ctatcgtctt
     3661 gcaggtcggt atcttcagta cgggaagagg ctacgcggtg ataaatacca taaaaaccga
     3721 ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg gagaggaacg
     3781 ccgccgccac gcaaaaagag agcgacagca gcattttgga gcgaatgctt aacagcaaac
     3841 gggtaaatat gaacagcaac agctgtttca taaaatagat acccgccacc ataaacatca
     3901 ataacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc gcgctggtca
     3961 tgccgatgaa taccgcttcg atagccaaca gaccaccggg gagcagcggg tagcatttca
     4021 gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagcccgcg acgaaagggc
     4081 tgatgaggaa aattaacggg tttacgatta agaaaatgat gagggcgagt ttgtaccagt
     4141 cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc tccatgatgg
     4201 tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa gcttaacggt
     4261 caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa aatgaagcct
     4321 tgatcccttt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg gtatgatgag
     4381 tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc gcaaagcccg
     4441 gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt ccctcccggg
     4501 actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg tactacgtta
     4561 cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca tggcaagccg
     4621 acgaaggtga ataatttctg ggaaacctct ggtttaaata tccttgaaac actggcgcga
     4681 ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg taccaatatt
     4741 tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca ggaagtgctg
     4801 gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga ttacaacata
     4861 ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct taacgggatg
     4921 aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc gcgcagtctg
     4981 gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca cgatcaggtg
     5041 tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg gatgcagaaa
     5101 aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa agagcaaacc
     5161 cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg ggcaacgttc
     5221 cagcaactcg atgctgccgt cttcgttttg ctgttcgagc atcacatcaa atccccacag
     5281 gcgatgcaca tgcttcagca cttctttgcg cccccgatcc agcggtgcgc gattatgtgg
     5341 aatgtaacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga tctgaatatt
     5401 cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct cccgataacc
     5461 ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcatcat ccagcacggt
     5521 gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa aactctcatc
     5581 tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg cgatatccgg
     5641 gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat cctggaacat
     5701 ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat aggggggctg
     5761 gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg ttactttccc
     5821 ttcatcatac agatggttaa ggatggtata gtgccagaag gtcgcccagc cttcgttcat
     5881 cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa tacgcaggat
     5941 ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga gcaggttttc
     6001 ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct cgcgcttcgg
     6061 cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgcgac ttttctgccg
     6121 ggctttctct tcttgcagtg agattttttg cgggcgtttg taccggtcca cgccgtagtt
     6181 catcagcgcg tggcacgagt ccagaagccg ttctacttca tcaacgccat aacgttcttc
     6241 gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac tggcgtcggt
     6301 ccaactgcgg aataagtaat tgtttttaaa gaaagagtta tgcccatagc aggcgtgtgc
     6361 catcaccagc gcctgcatgg taatggtgtt ctcttccatc aggtaagcga tacacgggtt
     6421 agagttaatg acgatttcat aggccagtcc ttgctgaccg tgcttataca gccgttcagt
     6481 ttcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc cgacgctgga
     6541 gtaggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt aggtatccag
     6601 ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca ggtcgaacgt
     6661 ccagtcgggt ccatcgctca aacgtgtggt gtccttattc atagaatcga tcgtcgccat
     6721 acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca agcatagaac
     6781 acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta ttatcagagt
     6841 gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt cagcaggaga
     6901 tactaaagac gccatattgc cgcagagtca gggagatgtg agccagctca ccataaaaaa
     6961 gccgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat tattattctt
     7021 ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga agtggtgtgg
     7081 taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc gtcattgatc
     7141 gggagccggg ggccgccctg gaaaccagcg ccgcgaatgc cgggcaaatc tcccccggat
     7201 atgctgcacc gtgggcggca ccaggtgtgc ctttaaaagc gatcaaatgg atgttccagc
     7261 gccatgcgcc gctggcggtc cgtctcgacg gtacgcagtt tcagttgaaa tggatgtggc
     7321 aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg atggtgcgtc
     7381 tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat attcagtatg
     7441 aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat gaaaatgcga
     7501 cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg gaatccagcc
     7561 gcctggcgga agtggagccc gcgctggcag aagtagcgca caaactgacg ggtggtctgc
     7621 aactgcccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg gcgcggatgg
     7681 cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg ctttgcgacg
     7741 gcgagcaaat ctacggcgtg aagtgtggcg atgaagtgat taaggccgat gcgtatgtga
     7801 tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt ccggtgtatc
     7861 cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg ccagtatcca
     7921 ctattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc attcgtgttg
     7981 gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt cgtgaaacgc
     8041 tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag gcgactttct
     8101 ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggcgt acacgcttta
     8161 aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct tgcggttccg
     8221 gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat gaggatctaa
     8281 gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta catggcgcac
     8341 acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg caggcattaa
     8401 aacagaatct gtccattgtc cgccaggccg cgccgcacgc gcgcgtctgg tcggtggtaa
     8461 aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg gccaccgatg
     8521 gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc tggaaggggc
     8581 cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc
     8641 gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa
     8701 aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc
     8761 ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt ggcgagatga
     8821 ccctgatgtc gcattttgcc gaagcggaac atcctgatgg aatttccagc gcgatggcgc
     8881 gtattgagca ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga
     8941 ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg
     9001 cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc
     9061 taagcagtga gattattggt gtccagacgc taaaagcggg tgagcgtgtg ggctacggcg
     9121 gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcaggg tacgccgacg
     9181 gttatccgcg ccacgcgcct accggtaccc ctgttttagt ggacggcgtg cgcaccatga
     9241 cggtggggac cgtctcgatg gatatgctag cggtcgattt aacgccttgc ccgcaggcgg
     9301 ggattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg
     9361 ctgccggaac ggtgggctat gagttgatgt gcgcgctggc gttacgcgtc ccggttgtga
     9421 cagtgtaact tgttgtaagc cggatcggag gcagcgtctt ctggttgcga aaaaatcatc
     9481 catccggctg gtcagcaact gtagttgtta atgtgacaga gccattgccc atgatagtgt
     9541 ccattaaaag aatggacact atttccccgg aacctgaact caccgcacag gcgttctaca
     9601 taaaacgctt acgcttcatt gttgactcga actcgacttc agataaatca cgctttaccc
     9661 ttgatggagc ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caattcaaca
     9721 tcaaatgaag gttaaattat ggacgacaaa caattgcagg ctcaggctgc gttcagcaaa
     9781 gcatcgcaac cggcgataga tgcttcatta aatttaagat tcagcttcct cttcagccac
     9841 ccgtacgcca atcttcaaca cttcattatc ttctttctcg gccaccgtcc agatcatccc
     9901 ggcaaactct acctggtcac cgacaaccgg tgccgcgcct aacaactgct ggacaatttc
     9961 acccagcgtt tgctgcttat cacgatattc tcgcccgtct tccaggccat atatcagcgc
    10021 aacatcagca tatttggcgc tggcttcgag aatgaagtca ccaaagaagc gttgatccag
    10081 cgcgaccggc ggcgactggc tgaacagttt accgagcgcc ggaagatcgc gttcccgacc
    10141 aattacacac aacacatcgc cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg
    10201 gttatcacga aacagtgccg caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc
    10261 cgcgcccacg caccatttat cggcactcaa ttgataaaca aactgctccc acggattttc
    10321 cggatgaata tccaggccaa cgcgtgacac cggacgtccc actggcggaa ccaccacttt
    10381 ggctttttta gccgcccacg agagtgatgt tccctgcaag agcagtgaaa ccagaaccac
    10441 aaagaaggcg acattaaaga acagacgtgc attctccagc cccgccatca tcgggaacac
    10501 agccaggatg attggcaccg cgccgcgtaa tcctacccag ctgataaaca cgcgctcacg
    10561 cagattgaaa ccgcggaagg ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa
    10621 tatcatccat gccgacaaaa tgagcgccgg aatggcaata ggcagcagat cgcttgggtt
    10681 aaccaacagc cccagcacca ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa
    10741 attttgcagg atgccgtagc ggttgcgaat cggccgatta cccagcagaa aaccgcacag
    10801 ataaacagcc agaataccgc tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc
    10861 gccgcttaat gccagcaatg gatataatcc ggcgggcagg gcgatgcgat taatcatttg
    10921 cagcagtaaa taaccaccac caagtccgat gacaatcccg aggccaaatt gctgcagaat
    10981 atcgaccacg aacatccaac tgacactgct ttcatgttgc tggatcatcg caatcagggt
    11041 aatcgtcaga aagaccgcca ttggatcatt actgccggat tcaatttcca gcgtcgagcc
    11101 aacacgttcg ttaagcccct taccacccag cagagaaaag accgctgcag catcggtaga
    11161 gccgacgata gcgccgatta ataagccttc aatcaaatca agattaaaca gccacgccgc
    11221 catcatgccg gttaaaccag aggtgataag cacgcccagc gtcgccagcg acagtgccgg
    11281 tcctaacgcc acacgaaagg aactggcctg agtgcgcatc ccgccgtcga gcaaaataat
    11341 cgccagtgcc aggttactta ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc
    11401 gacgccatcg actcctgcca gcatgccgat cgccagaaaa ataaccagaa taggaatgcc
    11461 aagacgggaa gaaaatgaac taagtaatat actgctggtg acaaggatgg atcctaagat
    11521 gaaaaggcta attattgttg tggcatccaa cggtcggtta ctcctgatta cgctgtctct
    11581 tatataaacc ctaccatatt agcggcagag acagcgtttt acttagccct gaagcgtgat
    11641 ttttttacat tttaagaaca ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt
    11701 tattcagaat ggcatgtgcg cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat
    11761 cgaggccggt aatgagtgga atcgacaggc gggaacgcaa aaacgcgtac actgactcca
    11821 ggttgtaacc tgcgtcataa tcattgggcg tgctgccgct aaagctaccg agaataatcg
    11881 ccttctgacg tggcaaaatt cccgcatgat agagctgcaa cagcatacgt tcgacgcgga
    11941 aagggtgctc gttaatatct tccagcacca gaataccgtt ctcaattttt ggcatccacg
    12001 gtgtaccaat cagtgaaatc agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct
    12061 gacatgtcgg gccttcccct tgccattcaa tggtgaaggt ttcattgcgt aacgccagcc
    12121 agaaatggtg ctcggtaaag gcgttcagtt catccgcgcc aaagtttgct accagcatgg
    12181 ggccgctaaa ggtgatgaca ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat
    12241 cgctatgccc gcatatgagc aacgggtcat attgttggcg agcaaccagt gcctgccagt
    12301 caatatctgc cagtaaacga ctggcaccgt aaccgccgcg tacagccagc acgatggtgt
    12361 tgggggtggt cagtctggca agggaattga gatcttccag gcgctccgtt tccgtacccg
    12421 caaaacgctc acaacgacgg gcaataacct cgacgttatt gacctgatgc ccctcgtcgg
    12481 taaggcgctg gataccacgc agcgcggcgt gctgtttaat gcagtaaccc gatggggcaa
    12541 ttaagtgaaa cagagacatg gcaattcctt gctgacaaca gaaacgaaat gtatatcatg
    12601 ccgcttaggt gtgccgttgt cacctcaacg gcgattccag gctataagga tagaagaagt
    12661 gaaattgaga tggtttgcct ttttgattgt gttattagcg ggttgttcat caaagcatga
    12721 ctatacgaac ccgccgtgga acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc
    12781 aataagccag aaagccggtg cagcctgggg cgtcgatcca caattgatca cggcgattat
    12841 cgctatcgaa tcgggtggta atccgaatgc ggtgagtaaa tcgaatgcca ttggtttgat
    12901 gcagttaaaa gcttcaacct ccggacgtga tgtttatcgc cgtatgggct ggagtggtga
    12961 gccgacgacc agcgagctga aaaatccaga gcgtaatatt tcaatggggg cggcttacct
    13021 gaatattctg gaaaccggcc cgctggcagg cattgaagat ccgaaggtac tgcaatatgc
    13081 gctggtggtg tcatacgcta acggggcagg tgcgctgcta cggactttct cgtcagatcg
    13141 gaaaaaggcg atcagcaaaa tcaacgattt ggatgctgac gagttcctcg atcacgtagc
    13201 gcgaaatcat cctgcgccgc aggctccgcg ctatatctac aaacttgagc aggcactgga
    13261 cgcgatgtaa atcagtcgcg cactttgtcc gctttttccc gggcttctcg ctcgagagag
    13321 aaaataatcc gctgtaattg ccgctccacc gtcgggctga cgttaagaaa acggaagctc
    13381 agacggggag tggtgatggt ttcattcttg ccatcaatca ctttgcgctc gctgatggag
    13441 attaactggg cgtcaaagtg aaaaacaccc cattgcccca tgttgacttc aatctgagcg
    13501 aagcgcatgc cttcttgtaa ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg
    13561 cctaacgaca aatcatacag gcggaaacgt aacgtactgt tatccgccag tttggtctgg
    13621 caaaaataag gcggatggag tggggcggag atgcggaaat atcggcgtcg ttgtacaaac
    13681 cataaggtgg gaggcggtac ggtgataaat gccggaagct gcaagtattc actctgctgt
    13741 agttgttcaa cagtaaactc gactttcgca ccctgagttt cggcggtaat ggtaatgtgc
    13801 tgtgccttta gcacggcgat gttgtcttcg gcttgactgc cgaaatccag caccagttta
    13861 tccggggtta ttgccagtat tttgctgatc agctgcccgc cattccaact gagacgcaaa
    13921 ggaattgcgg ctttgtgcaa atcgcgtaac acgcccagga cggctaacgg attttgtttc
    13981 aggaactgct catggtaatg actcacgcgg aaaaactcct gactgacaaa ctgtctttgg
    14041 tttatcggtc acagttaaca aaacttaata caaatgcgtg aatatttttt acatgttgtt
    14101 cttaaatcag ccgacatacg cccagcatcg atcccgccct tgcctatact tagagcgttg
    14161 acgtaagcat ttcttgcgtc cgattcatcg aaacgagggc ctgaacatgg gaattattgc
    14221 ctggattatt tttggcctga tagccggcat tatcgccaag ctaatcatgc cggggcgtga
    14281 tggtggtgga tttttcctga cctgtattct cgggatagtc ggtgcggtgg tcggcggctg
    14341 gctggcgacc atgtttggca ttggcggctc catcagtggt tttaatctgc acagcttcct
    14401 ggtggcggtg gtgggagcta ttctcgttct gggcgtattc cgcctcctgc gaagagaata
    14461 agattttcat aaggcggata gcgatacaga tgccgctatc cgctttcaca tcagaacgta
    14521 tactcgacac ctgctttacg ggtggaacaa atcaatatag cacttagatg cccgataaag
    14581 tcggttcccg gtaacaacaa cagaatcctt ttttaattat tgtttcgttg tttttggtgt
    14641 gattcagaaa ataaatattt tagacagtag ccagtcaggc gttaaaaatg gggcggatag
    14701 tgaggcggtt ttggctgcgc ttattgaaca tttagaattg atcaatcctt ctggcagatt
    14761 aagccaaaat acccgttcgg caatgcttca gttacgcgaa gaggaatggt ctgaattctt
    14821 tttttggttg ctaaattctc tggagtgtct ggattatgtg attattaatt tagctccaga
    14881 aagcaaaaaa acgttgatga gtgaacaccg taacaatatt caggtagcaa tcgatgcttt
    14941 atataggcaa cgtcggtgta aatcaccggg tgatgaaagt gaaacactta cccgtagaaa
    15001 tgacgctatc ttcggcaacc acgtatggca aacatttgcg caatacttcc cgccaggact
    15061 tgaaaaaccg tctgtgtaga aaacaccata gctatggtgt tatctgccga caacgccatc
    15121 cggagaagcc gaatgacgtt gtcggagtaa gcgctggtta aggtgtgggt tgtgcctctt
    15181 tggttgaggg ttgcgtcgtt gctgacttaa cggtcggacg cgtcgccgga acattgtcac
    15241 acggttgctc tttcgggcag atcaaatcca gcattttcag cgtcacgcca ttggtccagc
    15301 caaagccatc ctgtaatgga tattcgccac cgccgccccc cgttccggtg gtgctgacat
    15361 catatttttc caccagcttt ttctcccggt cataggtgtg ctgaacattg gtcaggaagt
    15421 gccagctaat gtccatcgcc acctcttttt gcccgtagtt ttgtaatcct tctgtcgcga
    15481 cccactgtaa cggtgcccag ccatttggcg catcccattg ttgcccactt ttcaccgacg
    15541 tggtgttcag gccgccgggt tgcagcagat gtgttttcgt cgccgtcgcc attttgttgg
    15601 cgcgatcttt cgctgccgca ttgacgtaca gcgggaacag ggcggccgcg gttaactgat
    15661 tgcgcacttt atgacttttc aggtcgtaat cggcatacca gccttgttga tcgttccaca
    15721 tgtatttttc gatccctttt tgacgggcat ttgccagcgt ttcgtactgg tttgccatcg
    15781 cgttatctcc ggcagctttg ctggcgcggg cgaggatttt ttccatttta aacatcaggc
    15841 tgttcagatc gaccggtacg atgctggtgg tgcgtaaggt atttagttga tgcgggttgt
    15901 ccatccagcg cgagctgaaa tcccagccag acgcagcggc agagcgcagg tcgcggtaaa
    15961 tttcagtggc aggtcgattc ggattgcttt tggcggtggc aatatcttcc acccatgact
    16021 ctggtcgtgg cgtatcgcga tcgtcccagt agcggttgag aagggtacca tcctgaagtt
    16081 ttacaacgcg tttttcctgt tgtccggctt gcaggttttc aacaccgtcc atccagtaag
    16141 catattcttt ttgcatttgc ggcaggtatt gcttcaacgc ggcatcgcct tcatgctgcg
    16201 ccagtaactc taccatcagg gcaaagaagg gcggttgcga gcggcttaaa tagtaactgc
    16261 ggttgccgtt gggaatatga ccgtaagtgt ctatttcatg agcaaaattg gccaccatat
    16321 ccgcgacttt atcccagtga ccgctttcgg caagtcctaa catggtgaag taactgtccc
    16381 agtaatatac ctcgcgaaaa cgtccgcccg gcacgacata aggttccggc agcggtaaca
    16441 gagaatccca tttttcggtg ttttcggtag aacgcgttaa taccggccaa agtccgtcaa
    16501 tatgttcgcg cagtgactgc ccctctggcg gaacatattt ctcgccctct ttcggcaggg
    16561 tgaaattgac gttaacgaaa tggcgcagat caaatccgct ctggttttgc tgcatccgat
    16621 aatcagcaag gatcatcagc ggatcgctgt tcggcacggc atcggcaaag gttttttggt
    16681 ccggaaaaag tttggcgttt tgcacatcat taaacagcgg ccctaataaa atatcaggcg
    16741 gctgtggtgt taccggtgtt tcttctgcct gcaccgatag cgcagcgaaa cacaaaaaga
    16801 tacaggctgg aattaacgcc attttttgcg ggcgagaagg tgcgggggat ttcatcaatc
    16861 attctccttt ggcgaaaccg aataaaacgg tattcaagta tcagaaaacc ttagttcagg
    16921 atcgcactag ctgcatgatc gaaatctcat tctgcgaact gtcaggcatt ttagaaaagg
    16981 gaattaaagg gataaataac ggtgaaatag agtgatcaaa taacgtctgt ttattagcca
    17041 gtcagcgacg ttgggttcgc gattagcgaa gaaaaatcat cataaattgg taaaaaatat
    17101 aacaggtagt taaaaaacat cagtgctggg taaattgccg gatgacatca gaacgatgcc
    17161 atccgaacgg tggcttaacc ctgacggttg aaacgttgcg ttttaacgtc cagcgttagc
    17221 gtttcttctg gttgtatcgc atacagtttc tcaccctgct ggcaaatcca gccaatcccc
    17281 agttcacggg cgattagggc gctgtgggat accggacttc cggcgctaag gcagatacct
    17341 tttacaaccg ccggatccag ttgcagtact gtggaaggat aaatgttctc cgccagtaga
    17401 atagttggcg agttaaactg cgggagttct tctttcgttt gggtcaggtg gaccagggtg
    17461 cgatgcagaa gatcgtccac atcaatatag cgagcttgta gatattcatc atccagttgc
    17521 tggtattgct ggctaagttc tttaagaact tgctgccagg catattctgc cgtgcaatgt
    17581 tcatgctgaa ggagttcgct tgccgccgcc agcagttccg gatcatctaa cagtgtatgg
    17641 tgaccagaaa agattgcggc aatatcgtca agcccgctgg cttctgcttt cgctgttaac
    17701 gtcatcagat ctaataacgt gaagtcaata gcctggcgta atcgatcttg ttcttcttcc
    17761 acggtcaggg ttgattttgc ctgtaccgta cataaaactg gttgataata aaaggcttta
    17821 cccgaaacag gcggaacggg acgcagagta ggtggagcga cttcctccgt ttcaccaaag
    17881 ttatcttcag ccagctgacg gaaagcgatc agtgcctctt cagcttctgg ccctttcgca
    17941 atcaggcgca gcgtatcgtt atagcgaact tgtagtaacg caatctggtt aatactctct
    18001 ggtgtgacgc atttgccgtt tttttccagc aacatatcgg cattaaatgt cgataaggta
    18061 taaaccagcc gggaggccgg acgtacatgc aggccgttac ggttttttat gaccaccgcc
    18121 agagaacggg cttcttcatc gtacgcagga catgtgtcag agatttcagt gtcggaggac
    18181 ggtaaaccca gttgttcacg tttggcttcc agcgcatgca tagcgtcaaa gataactttg
    18241 tcgatatccg ctcccgaggc cgcgctgacc gttgctgcca gtgtaccttc gaccaacggc
    18301 gcagcacaca aacgtacttt tgcggcgatc ttgggagcca gcaattccag cgcagtttca
    18361 gcactcaata atgcgctacc catatccatc atgaccagca catggtcggc atcagcaaca
    18421 gattcgatgg cctccatcac tttgacggca tcggtaccaa taggattttg tggatcgtca
    18481 attcccgcgg caatggcgat tttacaacta tcactcatta acatctgacg ggctaattca
    18541 ccgacacctt ctcccagtcg gctgctatgt gaaactatga ccaggtttac catcaccaat
    18601 tccttactct tttgcggcta acgccaacat ttgcatcata aacatcaccg aggtcgcgcc
    18661 gggatcctgg tgaccaatac tgcgttcacc gagataactg gcgcggcctt tgcgggcttg
    18721 catcgtaatc gtactttgtg cagcggattc ggcgattcta ctggcagctt cgagcgccac
    18781 cggaacagag agattttgct cgctggactg acgtaacgat tccaccaccg gcacccacac
    18841 atcacacatg gttttatcgc caggttcggc tttcccgcga ctgattacgc cgtccgcgcc
    18901 atcgcggaac atctgataaa gctcttccag tgtcaggctt tgccgtgcct gggtcgcctg
    18961 tgcggcgcgg ataaagaagg taccgaacag cggaccactg gcaccaccga cgctggaaag
    19021 cagcgtcata ccggtattct tgagaatgaa accgatatct ttatctgcga tagcagggag
    19081 tttttccacc actttgctaa agcctcggtt catatttagc ccgtggtcag cgtcgccaat
    19141 ttcgcgatcc agtccggtaa gatactcgct ctcggtgctg aaaatatcgc cacaacgagt
    19201 gagccagtta acaatttgag ttctgctcag tgacattgct ttctccttat ttaccccagt
    19261 taagggccgg ggtgtggacc ggggcgtccc agagtgccag cgtttcgtca tcaactttca
    19321 gtaaggtgat tgagaaaccg gtcatatcca gtgaggtgca gtacgcgcca attaaattac
    19381 gttcgatagt caatcccgct tgctggcaac gtgtggtcag gcggttatag acgccgtaca
    19441 gctcagaaag cggagttgcg ccaagattgt taaccagcgc aatcacccga tcgccagact
    19501 ggagcggttg tttggtttgt tgttcttcct gccaactgcc ttgttgataa tcccagaaac
    19561 gcaaagtgcg atggtatgag ccattttcca gcagggtgtc gaacatttca tcgacggttt
    19621 gatcaaggga agagaaggag cggcggtcaa tacccggctc accatgaatg ccgacgccaa
    19681 actccatctc attatccgcc agggtaaaag aaggtttgcc cgcggcagga acggtacagg
    19741 caccgagagc gatacctatt gagtggcctt gattattcaa cttacgcccc agttccgcac
    19801 aggcgtccag tgagtcgcca cgctccgccg ctgcgcctac gagtttttca attaatacgg
    19861 tgttggcaac gccgcgtcgc ccggcggtat aaagactgtc ttttaccgca acgtcgtcat
    19921 caatgaccac agtggtcact tttacgccgc tatcgtgcag taactcggtc gctgtttcaa
    19981 agttaagaat atcgccggtg taatttttga taatcaacag tacaccttcg ccgccatcaa
    20041 tttgcatgga gcattcaaag attttatcgg gcgtcggtga ggtgaaaatt tcgcccggac
    20101 aggcccccga aagcatcccc tgaccgatat aaccacagtg catcggctcg tgtccgctgc
    20161 cgccacccga cagcagggcg acttttcctg caacaggggc atcagctcgg gtgacataca
    20221 ccggatcctg atgcagtgtc agcgatggat gcgctttcgc cagtcctgcc agttgttcgt
    20281 ccagtacgtc ttgcacatca ttgatcaatt ttttcattat tttgctccag caattacggt
    20341 agggcatgga tgatgttcaa cgacacggcg accactgact gccgatgaat ccattgtgca
    20401 tcaggcaagg gaaaagaaaa ttagcccaaa ttatgtttca tagtgaaaca tatgctttaa
    20461 tgaatgttcc atattgaaac ttttacgtgt attaatactt aaaattgcga gccggaacac
    20521 cttttgtcat aagggatgcg ggatatgagt ggcgctttta acaacgatgg tcggggcata
    20581 tctcccttaa ttgcaacctc ctgggagcga tgcaataagc tgatgaaacg ggagacatgg
    20641 aacgtaccac atcaggccca gggcgtgaca tttgcttcta tttatcggcg taagaaagcg
    20701 atgctgacgc tcgggcaggc tgcgctggaa gatgcctggg aatatatggc accgcgagag
    20761 tgtgcgctgt ttatcctcga tgaaaccgcc tgcattctca gccgtaatgg cgatccgcaa
    20821 accttgcagc agctaagtgc actgggattc aatgacggca cgtattgcgc cgagggaatt
    20881 attggtactt gtgcgctatc gttagcggct atctctggtc aggccgtgaa aacgatggcc
    20941 gatcaacatt tcaaacaggc actctggaac tgggcctttt gtgcaacgcc gttgtttgac
    21001 agcaagggcc gattgacggg aacaatagcg ctggcgtgtc cggttgagca aactaccgca
    21061 gctgatttgc cgttgacgtt ggcaatcgcc cgcgaggtcg gaaatttact gctgacggac
    21121 agtttgctcg ctgaaactaa ccgtcattta aatcaactta atgccctgtt agaaagtatg
    21181 gatgatggcg tgattagctg ggacgagcag ggtaatttgc aatttattaa tgcccaggcg
    21241 gcgcgggtct tgcgccttga cgcgacggca agtcagggaa gggcaatcac tgaactctta
    21301 acgttacccg ccgtattgca acaagcaata aaacaggctc atccgctcaa acacgtagaa
    21361 gcaacctttg aaagtcagca tcagtttatt gatgcggtga taacccttaa accgataata
    21421 gaaacgcagg gaaccagctt tattttgttg ctccatcctg tggaacagat gcggcagttg
    21481 atgaccagtc aattaggaaa agtcagccat accttcgctc atatgccaca ggacgatccg
    21541 caaacccgcc gcttgattca ttttggtcgc caggcggcgc gcagtagctt tcctgtcctg
    21601 ctttgtggag aagagggcgt gggaaaggca ctgctaagtc aggcaattca taatgaaagc
    21661 gagcgtgctg caggtcctta tatcgccgtc aattgtgagt tatatggtga tgcggcgctg
    21721 gcggaagaat ttattggtgg cgatcgcacg gacaatgaaa atggccgtct gagtcggctg
    21781 gaactggcac acggcggcac gctgtttctt gaaaagattg aatatctggc ggtggagtta
    21841 cagtctgctt tgcttcaggt tatcaagcag ggtgttatca cgcgactgga tgcgcggcgt
    21901 ttaataccag ttgatgtcaa agtgattgcc acaacgaccg cggacctcgc aatgctggtg
    21961 gaacaaaatc gttttagtcg ccagctgtat tacgcgttgc atgcatttga aattaccatc
    22021 ccgcctctgc gtatgcggcg tggcagcatt ccggcgctgg tgaataacaa attacgcagt
    22081 cttgaaaaac gcttctctac gcggctgaaa attgatgacg atgccctcgc tcgcctggtt
    22141 tcctgtgcat ggcctggcaa cgattttgaa ctttacagcg tcatcgagaa tcttgctctg
    22201 agtagtgata atgggcgcat tcgcgtcagt gatttgccgg aacatctgtt taccgagcag
    22261 gcaacagatg atgtcagcgc cacacgcctt tccaccagtc tgtcatttgc ggaagttgaa
    22321 aaagaggcaa ttattaacgc agcccaggtc acaggcggtc gcattcagga aatgtcggct
    22381 ttacttggga tcggccgcac tacgctgtgg cggaaaatga agcaacatgg cattgatgca
    22441 gggcagttta agcgccgggt atgaaagaca gagacgattt ctgatacatc agagtgatct
    22501 atatttcatt ccggcgcacg ctaacatttt tcagcatcgt ttaagggctg gtctatcccg
    22561 cacttaaaag ctgaagcgat atcctccgtt gacttgcttc tgatcaaaga ggttactctg
    22621 cgtgtaatcc gcttcgaggt agaatgtgtg ctgtttgtta tactgtgcac tgactcccac
    22681 gccgttattc cagccattac ctttgaaact gtacttctcc cgggagttgt tcaacagata
    22741 gtctgtatcc cctgaaaact cgcggatagc tccagtcttc acatacatat tcagctggct
    22801 gttgcctgcg gtgatgtcat atcccagtat catgctggca cgccccagca gcgattcgta
    22861 gtgattcaga tgtatattga gaccattact cgccttcata gccatctcat tctggtggct
    22921 gtatgtgagc tgggtttgcg gctctatata gaacccataa ccagtagggg tcaggttgaa
    22981 cctctgcccg gcttccaggg agatgctcaa tccattcgca gtgccgttgg cgttaacgcc
    23041 gttgttctga ctgtccagta cgtgaaaact atttttctgg cgcgatgctt ttacaacgag
    23101 atcgctgtaa aaaccgttgt gtgccatgta actggcatac attcccatgt agtctgaacg
    23161 tgcggtaccg tcgcctccgc tatagtccgg cgatgcatgt gttgagccaa tatacagacc
    23221 gacatacaac ggcattacat cagagagacg tttatcccca ccaaactgga taccgctgta
    23281 acccatgtca aagccgctca gtttgccact ggcgaaggag tccaggcttc ccccataact
    23341 gcgcagccag atattaccgt ctttactctg atttcgcagg tcacccattc gttgcatcag
    23401 cgtacggttt tcaacatagt ttaataagta accgacattg agataattgc cgccagcatc
    23461 tgcggtcgtg gtgggtttag gtgtgggagt ggggttaggc gtaggatcgg gttcaggcgt
    23521 aggatcgggg ttgactatgg gaggctgagc gggagccggt gttggttcag gattaggagt
    23581 cggttccgga actgtcccgg aagcgtaaag ctcccagtta gtgccatttt tacgcacatc
    23641 gtacagatat ccccccaact caacctgaga agacgcgctg aacgaggccg caccgtcagt
    23701 ggtttttacc actgtcagaa cttcatttcc cgtagtggcc tcgctgccct ggttgcggat
    23761 agccaatacg tgattaccag cactacttcc gctgatattc aataaatccc ctttattatt
    23821 aacgccatta ccctcgccaa caacatcagc acgcataata aaggtactgt taccgctcag
    23881 gttctctacg tttaatgtgg caaatgtgcc ggcagttgac ccgtggctgg caaaatcgac
    23941 agttgaatgg ctcagcgcca gcgtgtcgag attagaatta cttgttacgt tccagacgct
    24001 gttattcatt gcaacgtcca gtttcccgcc attgacatta tcgctgaggg aggaacctgt
    24061 ccaaaccgac cctgggtgca tatctagatt gattagccca cctttggaaa gaacgctacc
    24121 gttgataagc atacgacccg aggcgttgat gcggctggcg gcataaccat cgtcatgttg
    24181 cgttgcaatg gccatctggt cgggtgtact catatcaatg accagatcgc tcgtgaggtc
    24241 gatctggctg ttggtcatgg cataaattcc cctggctcct gccgcgccgg tgatagccaa
    24301 actgtctccg gttattctac cgccgctgag cgcccacaaa cccagcgcca gactgccatt
    24361 acgatcaact gtaatatcgg tattttgcat gttgacaaca gccgttgccg tctgggccga
    24421 ggcaccataa gaaccgccgg aaaagatgct gtttcgttgc gctgccgtgc cagaaaaatt
    24481 gattgtcgcg tctgaaccac tggtgacgag gccaccgccc tgcgcggaag aaatatggct
    24541 atctgcaccg atagtggttg taccaccacg aacttcgacg ccattggccg cggctccagt
    24601 tacatcaaca gtgagtgcat tcgcgctaac ctggccaaag ctccagaggc cgtgtgcatt
    24661 atcgccattg gttttaatgg aactgtttgt tccgagatcg acaacagagt ttttctgtac
    24721 gtttatcccc atggcgctgt agccctgaac atcgattgtc aggtctgtag ccgtaaaacg
    24781 cgcagcacca ttggcgttat tgccgttgag accaccgata taaacacctg tacttccatc
    24841 ggtcgtgatt ttacttccgc ttccaagatc gacactggtg ccatagtcat tgatggttag
    24901 gcctataccg ttcgagtttt caatggtgaa ttgagtggct gttagtgttg agctatgccc
    24961 aataatgatg ccgtcatcgt tactcttaat ggtgctgcct gtgcctaagt cagcatgggt
    25021 atagtcacca attaagttaa tgccgatggc agaggtttga ccaacaacat ctactgtgag
    25081 tcggttagct gtcagggagc tgtttttgcc ctgaataatg attccttttg catatccaga
    25141 ggcgtcgttg acattgacgg tgacatcatt acccaggttg atattgccag gcgtgtttcc
    25201 tgctggggtc attacaccat agaggtttcc tgatggatcg gcggtatctc ctgtaattct
    25261 gtcaccttca ttaatagtaa tattggtgcc atattgactt cctgtatcag cagctatcag
    25321 caggggggaa aagataattg cattcaaacc aatagcgcca aattttatca gttgaattga
    25381 gggcgaacgg atttttaatt cagcgagacg atctgctttg gtattcccat tgtgttgttt
    25441 gatgcccata ccactcctat atagtaccca gactatgggt ctatggattt tgtcattttg
    25501 tttaaatcaa tgaataatat cctcttatca tcatgatatt tgtatggtgc aatcatgatg
    25561 agcttctcag agaataattc tctattaccg ggcaaaatca ggaacacagg aattaatata
    25621 tatttaacat cccccttaca aaggtgagtt tatatcgagt tattccctaa gttgtcgttg
    25681 cttattccaa ttcgttacta taaatatggc aaaaatatta caacaaacag cggttaaaaa
    25741 atttggtgaa gattttttgt aataatccta attagttatg ggaatttcgc ttgatgtatc
    25801 tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag caaaaaacat
    25861 cgatattcat ttttggtcaa tgggttttct ggcctatcgt tgttattgtg ctactggtta
    25921 tcattttcat cttgccagga tcgcacgtaa tcacgttatt cggggacttt atctgcggga
    25981 tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtgatc aatattgatc
    26041 agctgtgaag ggggtgatgt gtagtggacc aaaacttacg ttatatcact actaatgaga
    26101 tatccgtcat ctgacactga tgtctttaat tattctcgtg gttcactgta ggcaataagc
    26161 acaaaagtgt aggatgttac aagaatgatt aggactcggt gaaatgaaaa atccacgcaa
    26221 ttgcgtggat tatatatact tttgcgctct tcatgagatt tagcgaaacc tcatgagaca
    26281 acaaattaat tagacgttga aaaggaagtt catcacatcg ccatctttaa cgatgtaatc
    26341 tttaccttct gcacgcattt tgcctgcttc tttcgcgcct tgttcacctt tgtaagtgat
    26401 gaaatcttca aacgagatgg tttgtgcacg gataaagcct ttttcaaaat cagtatggat
    26461 tttgcccgct gcctgcggcg cggttgctcc aaccggaatg gtccatgcac gcacttcttt
    26521 caccccagcg gtgaagtaag tttgcaggtt cagcagctta taaccggcac ggatcacacg
    26581 gttcaggccc ggctcttcca gcccaagctc ctgcataaac tcgtcacgtt cttcgtcgtc
    26641 cagttcggca atgtctgctt caacagcagc acaaaccgga accacaacag aaccttcttt
    26701 cgccgcgatt tcacgcacct ggtcaagata tgggttgttt tcaaaaccgt cttcgttgac
    26761 gttggcgatg tacattgttg gtttcagcgt caggaagctc aggtaacgaa tagccgcttt
    26821 ctcttcagcg cttaaatcca gcgcgcgcag catacctgca ttttccaact ggggcaggca
    26881 tttttccagg accgccagct cagctttcgc gtctttatcg ccacctttgg ctttcttctg
    26941 tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc agcgccagtt cggtgttgat
    27001 aacttcaata tcgtcagccg ggttaacttt gccggaaacg tgaatgatgt tgtcattttc
    27061 aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg atgttggtca ggaactggtt
    27121 acccagacct tcgcctttcg atgcgccttt taccagaccg gcgatatcga caaattccat
    27181 ggtcgtggga agcgtacgtt gcggttttac gatttcagcc agttgatcca ggcgagggtc
    27241 aggcattggt acgacgcctg tgttcggctc aatggtgcag aatggaaagt tggccgcttc
    27301 aataccggct ttggtcagcg cgttgaacag ggtagatttc ccgacgttgg gcaaaccgac
    27361 gataccgcat ttgaatccca tgatttaaat caccttaata tcttaataat caacctgtta
    27421 ttgctaacag attgcagaaa tggaaataac tttgcctatt atacacggca ctcggcaaaa
    27481 atgccgcaga caacgactta ttgcgcttta aaggcgtgca atcggttcgt tgctttggtc
    27541 aagccatctg taaaccacat ttcagtacaa cgcgccgctt cgtcgatggc ttcatcaatt
    27601 aacttctgtt cactaacagg cggtttgcct aacacaaaac cgacaacttt atttttatcg
    27661 cccggatgac cgattccgat gcgtaaacgg tgaaagttag ggttattacc caatttactg
    27721 atgatgtctt tcagtccatt gtgaccacca tggccaccgc ccaatttaaa tttggcgacg
    27781 ccaggaggca gatccagttc gtcgtgggcc accagaattt cgtccggatt aatgcggaaa
    27841 aaactggcca tcgccgcaac ggctttgccg ctgagattca taaatgtagt cgggactaac
    27901 aggcggacat cttcgcctcc aagagtgact cgcgaagtat aaccaaagaa tttagcctct
    27961 tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa cgaaccaggc accagcatta
    28021 tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc cgacaatcaa tttaatcgtc
    28081 acgttttttt gtcctgagtg tgtacataac tggcgcgtag tttactggtt gcggccccgc
    28141 ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa ttgcatgagt ggactattag
    28201 aaagtcaagg tgttcaggcg tttatttgta aagttttgtt gaaataaggg ttgtaattgt
    28261 gatcacgccc gcacataacc cacagggtgt tgtctatact ttacacataa ggaagagggg
    28321 tattccctgt tacaacccag aaagttccgg aggtgacata tgaaacgcaa aaacgcttcg
    28381 ttactcggta acgtgctcat ggggttgggt ctggtggtaa tggtggtcgg cgtggggtat
    28441 tcaatcctca accagttacc acagtttaat atgccccagt atttcgcaca tggtgcagtg
    28501 ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg cgggcatgaa
    28561 caggtgtgcg accgttactg gtgggttcgc cactatgaca aacgttgccg ccgtagcgat
    28621 aatcgccgtc atagctaaca aataatgcag tttgctgacc agtcagtttg cgctgactgg
    28681 tcaatctcgt acttataaat ccgccatcgc cgcgcgacga ttcgggaaga acgccaggcg
    28741 tcccgggatc ggttgaatgc cagcgcgagc catagtgcgc agtggctgga attccacgtt
    28801 gcacacgcgc agttcacatc cctcgggcag acgcttcaca aaacgctgga acgcatcaag
    28861 accaccagca tcaagtaccg gaaccgcatc ccacttcaga atcacaatcc gtttgccttc
    28921 aagacgtgac tccaggtccg tgaacaagcc ttcagcagca gcaaaaaaca gcgggccaat
    28981 aacgcgcaga accaggacat cgtctggaac atctacgact accggtgcca ggcgagtcat
    29041 acgtgcgata cgacgcataa acagcagcga tgccagcacg atccccacgc tgatggcaat
    29101 aaccatatca aacagcacgg tcagcgacat gcacagcagc atgacaatga tgtcatcttt
    29161 cggcgcatga cgcagcaagt cgaccacttt gtgcgcttca ctcatgttcc acgccaccat
    29221 caacagcagg gcagccatgg cggaaagcgg cagccaggag agcagcggtg ccagtaccag
    29281 cagggcaaga ataaccagaa tagagtggat caccgccgag ataggggaag ttgccccggc
    29341 acggacgtta gcggcagaac gcgcgatggc agctgtagcg gtaatgccac caaagaacgg
    29401 agcgatgata ttccccagtc cctgtccaac cagttcgcta ttcgccttgt gtttcgtccc
    29461 ggtcatacca tccagcacca cggcgcagag cagagattcg attgcgccga gcattgccat
    29521 tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag gttagcgtga attctgaatt
    29581 aggcagatcc cacggcagca ccagttgcgg cagcagttgc ggaataccgt taccctgaga
    29641 accatcggcc aggacgtagt ggaattgcga accgatggta gcaacatgtc cgccgagcag
    29701 gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac cgggtaaacg
    29761 aatgcccaga cgcggccaga aaacaagaat acctagcgtc acaatgccaa tggcagcatc
    29821 acccacatta atggtcggca gcgccataaa taatgcgccg actttttgta gataatgttc
    29881 cgggacatgg gccatttgca gaccgagaaa atctttaatc tgcatggtac cgatggtgat
    29941 cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa tcaggcgacc
    30001 aaagcgtgcc agacccatca gaatcaaaaa aatccccgac agcaaggtcg caaccagcag
    30061 tcctgccagt ccaaactgtt gcgacacggg atagagaatt accacaaatg ccgcagtcgg
    30121 accggaaacg ctaaagcgtg acccacccgt cagagcaatg acaatccccg caacagctgc
    30181 ggtatataaa ccgtactggg gtgccacacc actaccaata gccaacgcca tcgccagcgg
    30241 gatagcaata atcccgacgg ttatcccggc aatcaggtca cgggtaaacc gtgcggcagt
    30301 atatttttct ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat gtgaggaaaa
    30361 tattttgttc acaataatgt ttcatccgtg agcgcatcat ctgtcaacta aatggcaggt
    30421 gaaggaggca taggtcatac aaatggatat tacagacaaa aaaacccgcc gcagcgggtc
    30481 tttgagccgg gttcgattag tgttcgaaca tggcagagat cgattcttcg ttgctgatac
    30541 gacgaatcgc ttcggccagc atacctgaca gggtcagagt acgcacgttc ggcagtgatt
    30601 tgatttcatc gctcagcgga atggtatcgc agacaacgac ttcatcaatt acagagttac
    30661 gcaggttatt cgccgcgttg ccagagaaga tcgggtgagt cgcgtacgca aatacacgtt
    30721 tagcaccacg ttctttcaga gcttcagcag ctttacacag cgtaccgcca gtgtcgatca
    30781 tatcatcgac cagtacgcag tcacgacctg caacgtcacc gatgatatgc atcacctggg
    30841 aaacgttcgc acgcggacga cgtttgtcga tgattgccat atcggtatcg ttcagcagct
    30901 tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg agaaaccaca attgggttat
    30961 ccagattcag ctgcagcatg tcttccagca ggatcgggct accaaatacg ttatcaaccg
    31021 gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc cactgtcagc acacggtcaa
    31081 caccgacgct ggagaggaag tctgcaacca ctttcgcagt gattggtaca cgagcggaac
    31141 ggacgcgacg gtcctggcgc gcatagccaa agtaggggat aacagcggtg atacgacctg
    31201 cggaagcacg acgcagggca tcaaccataa cgactaattc catcaggttg tcgttagtag
    31261 gggcacaagt ggactggatg atgaaaatat caccaccgcg tacattttcg ttgatttgta
    31321 cactgacttc gccgtcgcta aagcgaccta cagcggcgtc gccgagtgaa gtgtacaggc
    31381 ggttggcaat acgttgtgct agttccgggg tggcgttacc agcaaaaagc ttcatatcag
    31441 gcacgagaag aacctcaggc atgcgtccat tggtggaaag aatctgccga aaactgtgcg
    31501 ggccaggcat gatcctttcc aggcggtgta ttaaagagcg cgatgcaacg tctggaacaa
    31561 ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag catggctctg tgcaatgggg
    31621 aaagattaac gcctttcgcc acaaagccat tgagccattc cggggcttgc tctagcacct
    31681 ggcgggcttc agactctgta tcaaattcag caaagacaca ggcccctgtc ccagtcaggc
    31741 gcgacggggc gtattctaac agccaggaaa gcaccgcatc aacctcgcga aaacgttttc
    31801 ttgcgataac ctcgcaatca ttgctgaatt cacattttag caacgtttct attgaccttt
    31861 ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac cggagtcgga atacttacac
    31921 cagggtgcgc caccagatac cacttctctg gcggatccac cggcgttagt atttcaccaa
    31981 cgccttcggc aaacgaggca tgcccccgaa caaagacagg aacatctgcg cccagcgtca
    32041 gccccatttc cgccagctca tccatgctta gcccgcattg ccagagatga tttaatgcca
    32101 ccaggaccgt cgcggcattg gatgaaccac cgccgagacc gccgcccatc ggcaaacgct
    32161 tgtcaatgct gatattcgca ccgcttcccg tcggaagacg cccgctgtct gccgcagttt
    32221 tcatcaacaa tcgcgctgcg cgaacgatca ggttatcttc atgttccacg ccttcaacgg
    32281 gcgttaacag acgaatatcc ccatcgtcac gaagctcaat gctgatggtg tcgccgtaat
    32341 caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc cgcacgctga ccggtaatgt
    32401 ataaaaacag attaagtttt gccggagagg gccactgtgt ccgcattatt tcactatcca
    32461 gttatccatt tttaacttga tgcgttgacc accgtcggtg agttccatat tggctggcat
    32521 cgcaggttgc gttttggtgt cataaccacc ataaacaacc ttccagtttt tgccattctg
    32581 gctgtaggta atttcgctca ggcggtactg gtcgtccagt ttgtagtcgg ttgcatcacc
    32641 cggtaaacct aaaatccact ggcgcaagct gttgagcgga attggcattc cggtcaattt
    32701 gccaatcatc tcttcggcgt catcggcggt ataacgctga cctttattgt cgactaactg
    32761 cacgttaccc ggttgagcat tcagctccag ttccgtgctg cccaatgggt tagtgagcag
    32821 cagacggtag cgatcctggc cggtttgctg ccagaaaaag cgggcgtaca ctttttgttg
    32881 gtcagaaata taagcgaacg cgccgcgagt ctgatactga ttaagattgc gcacgtcttg
    32941 ctgatgctga cgccattgtg gcgaatccgg gcttttgcca ggacctttgg gcgtggtaac
    33001 ggaacaggca gtgagcacaa gagcagccag cggtagcagg cggataagac gaaaatcggg
    33061 caggggcata gtgatgacaa gtccttgaga tacgttgcag ttataaccct taatgctagc
    33121 gttaccgtcc gctatcgtct atgttcaagt tgtcttaatt gccagaatct aacggctttc
    33181 ggcaattact ccaaaagggg gcgctctctt ttattgatct tacgtatcct gtatgatgca
    33241 agcagactaa ccctatcaac gttggtatta tttcccgcag acatgaccct tttagcactc
    33301 ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac gtgtatcgtt ttcgccggat
    33361 aagctcgatc aggcgcttga cagcctgctt gcgcagccga tggtgcaggg cggcgtggtg
    33421 ctgtcgacgt gcaaccgcac ggaactttat cttagcgttg aagagcagga taacctgcaa
    33481 gaggcgttaa tccgctggct ttgcgattat cacaatctta atgaagaaga tctgcgtaaa
    33541 agcctctact ggcatcagga taacgacgcg gttagccatt taatgcgtgt tgccagcggc
    33601 ctagattcac tggttcttgg ggagccgcag atcctcggtc aggttaaaaa agcgtttgcc
    33661 gattcgcaaa aaggtcatat gaaggccagc gaactggaac gcatgttcca gaaatctttc
    33721 tctgtagcga aacgcgttcg cactgaaaca gatatcggtg ccagtgctgt gtctgtcgct
    33781 tttgcggctt gtacgctggc gcggcagatc tttgaatcgc tctctacggt cacagtgttg
    33841 ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc atctgcgcga acataaagta
    33901 cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc aaattctggc agatgaagtt
    33961 ggcgcggaag tgattgccct gagtgagatc gacgaacgtc tgcgcgaagc cgatatcatc
    34021 atcagttcca ccgccagccc gttaccgatt atcgggaaag gcatggtgga gcgcgcatta
    34081 aaaagccgtc gcaaccaacc aatgctgttg gtggatattg ccgttccgcg cgatgttgag
    34141 ccggaagttg gcaaactggc gaatgcttat ctttatagtg tggacgatct gcaaagcatc
    34201 atttcgcaca acctggcgca gcgtaaagcc gcagcggttg aggcggaaac tattgtcgct
    34261 caggaaacca gcgaatttat ggcgtggctg cgagcacaaa gcgccagcga aaccattcgc
    34321 gagtatcgca gccaggcaga gcacgttcgc gatgagttaa ccgccaaagc gttagcggcc
    34381 cttgagcagg gcggcgacgc gcaagccatt atgcaggatc tggcatggaa actgactaac
    34441 cgcttgatcc atgcgccaac gaaatcactt caacaggccg cccgtgacgg ggataacgaa
    34501 cgcctgaata ttctgcgcga cagcctcggg ctggagtagc agtacatcat tttctttttt
    34561 tacagggtgc atttacgcct atgaagcctt ctatcgttgc caaactggaa gccctgcatg
    34621 aacgccatga agaagttcag gcgttgctgg gtgacgcgca aactatcgcc gaccaggaac
    34681 gttttcgcgc attatcacgc gaatatgcgc agttaagtga tgtttcgcgc tgttttaccg
    34741 actggcaaca ggttcaggaa gatatcgaaa ccgcacagat gatgctcgat gatcctgaaa
    34801 tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga aaaaagcgag caactggaac
    34861 agcaattaca ggttctgtta ctgccaaaag atcctgatga cgaacgtaac gccttcctcg
    34921 aagtccgagc cggaaccggc ggcgacgaag cggcgctgtt cgcaggcgat ctgttccgta
    34981 tgtacagccg ttacgccgaa gcccgccgct ggcgggtaga aatcatgagc gccagcgagg
    35041 gtgaacatgg tggttataaa gagatcatcg ccaaaattag cggtgatggt gtgtatggtc
    35101 gtctgaaatt tgaatctggc ggtcatcgcg tgcagcgtgt tcctgctacg gaatcgcagg
    35161 gtcgtattca tacttctgct tgtaccgttg cggtaatgcc agaactgcct gacgcagaac
    35221 tgccggacat caacccagca gatttacgca ttgatacttt ccgctcgtca ggggcgggtg
    35281 gtcagcacgt taacaccacc gattcggcaa ttcgtattac tcacttgccg accgggattg
    35341 ttgttgaatg tcaggatgaa cgttcacaac ataaaaacaa agcgaaggcg ctttcagtgc
    35401 tcggtgctcg catccacgct gctgaaatgg caaaacgcca acaggccgaa gcgtctaccc
    35461 gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa ccgtacttac aatttcccgc
    35521 aggggcgcgt taccgatcac cgcatcaacc tgacgctcta ccgcctggat gaagtgatgg
    35581 aaggtaagct ggatatgctg attgaaccga ttatccagga acatcaggcc gaccaactgg
    35641 cggcgttgtc cgagcaggaa taatggaata tcaacactgg ttacgtgaag caataagcca
    35701 acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc ctgctggagc atgttaccgg
    35761 cagagggcgt acttttattc tcgcctttgg tgaaacgcag ctgactgacg aacaatgtca
    35821 gcaacttgat gcgctactga cacgtcgtcg cgatggtgaa cccattgctc atttaaccgg
    35881 ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca gcgaccttaa ttccgcgccc
    35941 ggatacggag tgtctggtgg agcaggcact ggcgcggttg cctgaacaac cttgccgtat
    36001 tcttgatctc gggacgggta ccggggcgat tgcgcttgcg ctggctagcg agcgcccgga
    36061 ctgcgaaatt atcgctgtag atcgtatgcc tgatgctgtc tccctggcac aacgtaatgc
    36121 ccagcatctg gcgatcaaaa atatccacat tctgcaaagc gactggttta gcgcgctagc
    36181 cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat attgacgagc aggacccaca
    36241 tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg ctggttgcgg cagacagtgg
    36301 aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac gcgctggtat ccggcggctt
    36361 tctgcttctg gaacatggct ggcagcaggg cgaagcggtg cgacaagcat ttatcctcgc
    36421 ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat aacgagcgcg taacgctcgg
    36481 ccgctattat caatgacaag tttttctaca ctgcttagtg ttcatcttat tagtatcgcg
    36541 ctttctgttg ggctattaac cttacgtttc tggctacgtt atcagaagca tccacaggca
    36601 tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata cggtgttatt gttaagcggc
    36661 attgcgttga tggctaaagc gcacatcctg ccattttccg ggcaggcaca gtggctgact
    36721 gaaaagctgt ttggagttat catttatatc gttttgggtt ttattgcact cgattatcgt
    36781 cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc tggcgttggt ggtgctgtac
    36841 atcatcatta aactcgccac cacaaaagta ccgttactgg ggtaagtcat gagatcgtta
    36901 gctgatttcg aatttaataa agcgccattg tgcgaaggca tgatcctggc ttgcgaagca
    36961 atccgccgcg attttccctc gcaagatgtt tacgacgaac tggagcgtct cgttagtctg
    37021 gcgaaggaag aaatcagcca gcttctgcct ttagaagagc agctggaaaa actgatcgcg
    37081 ctgttttacg gcgactgggg atttaaagcc tcacgcggcg tttatcgtct ttccgatgca
    37141 ttatggctgg accaggtgtt aaagaatcga cagggcagtg cggtatcatt aggtgcggtt
    37201 ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc cggtgatttt ccctacgcag
    37261 ctgatattgc gcattgaatg tccggatggc gaaatttggc tgattaatcc ttttaacggt
    37321 gaatcgttaa gcgaacatat gctggacgta tggttaaagg gaaatatcag cccgtcggcg
    37381 gaactgtttt atgaagacct tgatgaagct gataacattg aggtaatccg caaattgctg
    37441 gatacactca aagcctcgtt gatggaagaa aatcagatgg agctggcgtt acgcaccagc
    37501 gaagctttat tacaattcaa ccctgaagat ccctatgaaa ttcgcgatcg cgggttgatt
    37561 tatgtgcaac tggattgcga acacgttgcg ttgaacgatt taagttattt cgttgaacag
    37621 tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa taaataacat cgcgcataaa
    37681 catattgtgc tgcattaatt aatcgacatt ttactcaaga ttaaggcgat cctatgaaac
    37741 aaaaagtggt tagcattggc gacatcaacg tagcaaatga cctgccgttc gtactgtttg
    37801 gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg catttgcgag cactacgtaa
    37861 ctgtgaccca gaaactgggt atcccttacg tgttcaaagc ctcttttgac aaagccaacc
    37921 gctcctccat ccactcttat cgtggaccgg gcctggaaga agggatgaaa atcttccagg
    37981 agttgaagca gacttttggc gtgaaaatta tcaccgacgt tcacgaacca agtcaggcac
    38041 agcccgttgc tgatgtcgtg gatgtgattc agttgccggc gtttcttgct cgccagactg
    38101 acctggttga agccatggcg aaaaccggtg cggtaattaa cgtcaagaaa ccacagtttg
    38161 tcagcccggg acagatgggt aatatcgttg ataaattcaa agaaggcggc aacgaaaaag
    38221 tgattctttg cgatcgtggt gctaacttcg gctatgacaa cctggttgtc gatatgctgg
    38281 gcttcagcat tatgaagaaa gtgtctggta actcgccggt gattttcgac gtgacccacg
    38341 cactgcaatg ccgcgatccg tttggcgcag cttccggtgg tcgtcgtgct caggtggctg
    38401 agctggcacg agccggtatg gcggtaggtc tggcggggct gtttattgaa gcgcatccgg
    38461 atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc gctggctaaa ctggaaccgt
    38521 tcctcaagca gatgaaagcg attgatgatc tggtgaaagg tttcgaagaa ctggatacca
    38581 gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt tctgcgttaa gacttttttc
    38641 atgggtgcca gatacaaaaa aggccgcagg ctgttacccc tgcggccggt ttcgggcgca
    38701 tattgccatc acggcagcct gacgcccgtt ttcaccttac ttccggttac gccaccagcc
    38761 gacaatcgct gcggtaataa ttcccgccag gatcggtgct gccaggtcgt gccagaaaat
    38821 catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca ttttgcggct
    38881 attcttgaat tgttctggtt caagattagc ccccgttctg ttgtcaggtt aaacctctca
    38941 acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca
    39001 ttgcgcctca ccggataacg ccggcttgat gtggatacta cgtctcaatt catcttcact
    39061 tcatccctga aatgtttgca atgaagagtg cattccggtt tttcaacagc tgttacagtc
    39121 gtttcatgag ggctctggat gatgcttcca gctcgggttg ccaatgttta ctggcggaag
    39181 agataaagac aaaaaaggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg
    39241 ccatcacggc agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa
    39301 tcgctgcggt aataattccc gccaggatcg atgctgccag gtcgtgccag aaaatcatgg
    39361 caaactgcgc gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct
    39421 tgaattgttg atggttcaag attagccccc gtt
//
LOCUS       DAZFKM010000029        39206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFKM010000029 DAZFKM010000000
VERSION     DAZFKM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(141..332)
                     /gene="yfgG"
                     /locus_tag="ACHKAY_001664"
     CDS             complement(141..332)
                     /gene="yfgG"
                     /locus_tag="ACHKAY_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO5700358.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            350..502
                     /locus_tag="ACHKAY_001665"
     CDS             350..502
                     /locus_tag="ACHKAY_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700359.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            684..2927
                     /gene="pdeF"
                     /locus_tag="ACHKAY_001666"
     CDS             684..2927
                     /gene="pdeF"
                     /locus_tag="ACHKAY_001666"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO5700360.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2966..4507)
                     /gene="ppx"
                     /locus_tag="ACHKAY_001667"
     CDS             complement(2966..4507)
                     /gene="ppx"
                     /locus_tag="ACHKAY_001667"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO5700361.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4512..6578)
                     /gene="ppk1"
                     /locus_tag="ACHKAY_001668"
     CDS             complement(4512..6578)
                     /gene="ppk1"
                     /locus_tag="ACHKAY_001668"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO5700362.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6749..7387)
                     /gene="purN"
                     /locus_tag="ACHKAY_001669"
     CDS             complement(6749..7387)
                     /gene="purN"
                     /locus_tag="ACHKAY_001669"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO5700363.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7387..8424)
                     /gene="purM"
                     /locus_tag="ACHKAY_001670"
     CDS             complement(7387..8424)
                     /gene="purM"
                     /locus_tag="ACHKAY_001670"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO5700364.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8749..9375
                     /gene="upp"
                     /locus_tag="ACHKAY_001671"
     CDS             8749..9375
                     /gene="upp"
                     /locus_tag="ACHKAY_001671"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO5700365.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9461..10750
                     /gene="uraA"
                     /locus_tag="ACHKAY_001672"
     CDS             9461..10750
                     /gene="uraA"
                     /locus_tag="ACHKAY_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO5700366.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10845..11546
                     /gene="hda"
                     /locus_tag="ACHKAY_001673"
     CDS             10845..11546
                     /gene="hda"
                     /locus_tag="ACHKAY_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO5700367.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11684..12043)
                     /gene="arsC"
                     /locus_tag="ACHKAY_001674"
     CDS             complement(11684..12043)
                     /gene="arsC"
                     /locus_tag="ACHKAY_001674"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO5700368.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12064..13527)
                     /gene="bepA"
                     /locus_tag="ACHKAY_001675"
     CDS             complement(12064..13527)
                     /gene="bepA"
                     /locus_tag="ACHKAY_001675"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO5700369.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13740..14801
                     /gene="yfgO"
                     /locus_tag="ACHKAY_001676"
     CDS             13740..14801
                     /gene="yfgO"
                     /locus_tag="ACHKAY_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5700370.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14839..15687)
                     /gene="focB"
                     /locus_tag="ACHKAY_001677"
     CDS             complement(14839..15687)
                     /gene="focB"
                     /locus_tag="ACHKAY_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO5700371.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15709..17721)
                     /gene="hyfR"
                     /locus_tag="ACHKAY_001678"
     CDS             complement(15709..17721)
                     /gene="hyfR"
                     /locus_tag="ACHKAY_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO5700372.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17751..18164)
                     /gene="hyfJ"
                     /locus_tag="ACHKAY_001679"
     CDS             complement(17751..18164)
                     /gene="hyfJ"
                     /locus_tag="ACHKAY_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO5700373.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18157..18914)
                     /gene="hyfI"
                     /locus_tag="ACHKAY_001680"
                     /pseudo
     CDS             complement(18157..18914)
                     /gene="hyfI"
                     /locus_tag="ACHKAY_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18911..19456)
                     /gene="hyfH"
                     /locus_tag="ACHKAY_001681"
     CDS             complement(18911..19456)
                     /gene="hyfH"
                     /locus_tag="ACHKAY_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO5700374.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19466..21133)
                     /gene="hyfG"
                     /locus_tag="ACHKAY_001682"
     CDS             complement(19466..21133)
                     /gene="hyfG"
                     /locus_tag="ACHKAY_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO5700375.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21123..22703)
                     /gene="hyfF"
                     /locus_tag="ACHKAY_001683"
     CDS             complement(21123..22703)
                     /gene="hyfF"
                     /locus_tag="ACHKAY_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO5700376.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22708..23358)
                     /gene="hyfE"
                     /locus_tag="ACHKAY_001684"
     CDS             complement(22708..23358)
                     /gene="hyfE"
                     /locus_tag="ACHKAY_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO5700377.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23370..24809)
                     /gene="hyfD"
                     /locus_tag="ACHKAY_001685"
     CDS             complement(23370..24809)
                     /gene="hyfD"
                     /locus_tag="ACHKAY_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO5700378.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24826..25773)
                     /gene="hyfC"
                     /locus_tag="ACHKAY_001686"
     CDS             complement(24826..25773)
                     /gene="hyfC"
                     /locus_tag="ACHKAY_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO5700379.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25784..27802)
                     /gene="hyfB"
                     /locus_tag="ACHKAY_001687"
     CDS             complement(25784..27802)
                     /gene="hyfB"
                     /locus_tag="ACHKAY_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO5700380.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27802..28419)
                     /gene="hyfA"
                     /locus_tag="ACHKAY_001688"
     CDS             complement(27802..28419)
                     /gene="hyfA"
                     /locus_tag="ACHKAY_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO5700381.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28672..29142)
                     /gene="bcp"
                     /locus_tag="ACHKAY_001689"
     CDS             complement(28672..29142)
                     /gene="bcp"
                     /locus_tag="ACHKAY_001689"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO5700382.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29142..29714)
                     /gene="gcvR"
                     /locus_tag="ACHKAY_001690"
     CDS             complement(29142..29714)
                     /gene="gcvR"
                     /locus_tag="ACHKAY_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO5700383.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29860..30738
                     /gene="dapA"
                     /locus_tag="ACHKAY_001691"
     CDS             29860..30738
                     /gene="dapA"
                     /locus_tag="ACHKAY_001691"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO5700384.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30755..31789
                     /gene="bamC"
                     /locus_tag="ACHKAY_001692"
     CDS             30755..31789
                     /gene="bamC"
                     /locus_tag="ACHKAY_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO5700385.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            32002..32715
                     /gene="purC"
                     /locus_tag="ACHKAY_001693"
     CDS             32002..32715
                     /gene="purC"
                     /locus_tag="ACHKAY_001693"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO5700386.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32869..33096
                     /gene="vapB"
                     /locus_tag="ACHKAY_001694"
     CDS             32869..33096
                     /gene="vapB"
                     /locus_tag="ACHKAY_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO5700387.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33096..33494
                     /locus_tag="ACHKAY_001695"
     CDS             33096..33494
                     /locus_tag="ACHKAY_001695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO5700388.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33641..34504
                     /gene="ypfJ"
                     /locus_tag="ACHKAY_001696"
     CDS             33641..34504
                     /gene="ypfJ"
                     /locus_tag="ACHKAY_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO5700389.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34519..36534
                     /gene="tmcA"
                     /locus_tag="ACHKAY_001697"
     CDS             34519..36534
                     /gene="tmcA"
                     /locus_tag="ACHKAY_001697"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO5700390.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36608..37306
                     /gene="ypfH"
                     /locus_tag="ACHKAY_001698"
     CDS             36608..37306
                     /gene="ypfH"
                     /locus_tag="ACHKAY_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5700391.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37416..37616)
                     /gene="ypfN"
                     /locus_tag="ACHKAY_001699"
     CDS             complement(37416..37616)
                     /gene="ypfN"
                     /locus_tag="ACHKAY_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO5700392.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37644..38771)
                     /gene="dapE"
                     /locus_tag="ACHKAY_001700"
     CDS             complement(37644..38771)
                     /gene="dapE"
                     /locus_tag="ACHKAY_001700"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO5700393.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(38775..39131)
                     /gene="yffB"
                     /locus_tag="ACHKAY_001701"
     CDS             complement(38775..39131)
                     /gene="yffB"
                     /locus_tag="ACHKAY_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO5700394.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9596 a        10036 c        10297 g         9277 t
ORIGIN      
        1 gccaaacagg ataaacatca gtcaggacaa agacgtaaaa tttaagatga aagaatatct
       61 gccgctttga taattccaaa gagcaaaagc atccgacagc gaagtgccgt taggttctgt
      121 gaaagtggag aaggtaaccg ctaacgttgt accggtgatt cgacggagag tgtggattgc
      181 tgagcttctt ttttgctctg atggtgctgc caggcaccga cggacgagta gataaaacgg
      241 ccaaagaaga agataaagct gatgagcagt acgatacggg tcatgcgact gttaaatcgg
      301 tgtcgttttc gcatactggt tgcctgactc acaaaaggtt ccttgaagta tgtcccacgc
      361 tgtggacggt acttacatta aggcacaaca ggacaaaatg gtcaattctt cgttatgtaa
      421 aaaagcgtca gtggatacat attttaatgt tatggaagtt aatttaatta tttacaatga
      481 tgatgtaata atgatgaatt aacatgataa acgtaataat tcattaatca gggttatttt
      541 gtttgatata tatcaattgg aactttcata tgacacttag aatcaatgct ctctatagtg
      601 atgaataatc atcattcgaa gtcaggtggg atgcctgtct gaatacacct ccttcaggat
      661 gtgggggatt ctgctgagca tctatgaaac tgaatgcaac ttatataaaa atacgtgata
      721 aatggtgggg gcttccgctg ttcctgcctt ctttaatctt gcccattttc gcccacatta
      781 atactttcgc gcatatttct tccggtgagg tttttctctt ttatctgcct ttggcactga
      841 tgatcagcat gatgatgttt ttcagctggg cggcattgcc agggatcacc ttagggattt
      901 ttgtccgcaa atatgcagag ctgggttttt acgaaacgct ctcattaacg gctaatttta
      961 ttatcattat cattctctgt tggggcggtt acagggtctt tactccccgg cgtaacaacg
     1021 tttcacatgg tgatacccgt ttaatttccc agcgtatatt ctggcagatt gtgtttcctg
     1081 caacgctgtt tctgatactt ttccagtttg ctgcatttgt aggattactg gcgagcagag
     1141 aaaatctggt cggtgtcatg ccttttaacc tcgggacttt aatcaattat caggccttac
     1201 tggtgggtaa tctgatcggt gtcccgctgt gctacttcat cattcgggta gtgcgaaatc
     1261 cattttattt acgtagctat tattcgcaat taaaacagca ggttgatgcc aaagtcacca
     1321 aaaaagagtt cgcgctctgg ctactggcat taggtgcttt attgttgctg ttatgcatgc
     1381 cgttaaatga aaaaagcaca atttttagca ccaattatac cttgtcatta ttgctgcccc
     1441 tgatgatgtg gggagcgatg cgctatggtt ataagctgat ttcgctgctc tgggcggtcg
     1501 tgttgatgat cagcatccac agctatcaaa attacattcc catttatcct ggctatacca
     1561 cgcagctgac cataacctcc tccagttatc tggtattctc ttttattgtc aattatatgg
     1621 ctgtactggc aactcgtcag cgagcggtag tcagacgcat tcagcggctt gcgtatgtgg
     1681 acccggtggt tcatctgcca aatgttcgcg ccctgaatcg cgcgttgcgt gatgccccct
     1741 ggtctgcgct ttgttattta cgcatccctg gcatggaaat gctggttaag aactatggca
     1801 tcatgctgcg gattcaatac aagcaaaaac tttctcactg gctgtcaccc ttgctggaac
     1861 cgggtgaaga tgtttatcag ctttcgggta acgatctcgc gctgcggctg aatacagaat
     1921 cgcaccagga gcgcattacc gcactggata gccatctcaa gcaatttcgt ttcttttggg
     1981 atggcatgcc gatgcaaccg cagattggcg tcagttactg ctatgtgcgc tcgccagtga
     2041 atcatatcta cctgctgctg ggagagctaa atacggtcgc cgaactttcc atcgtgacca
     2101 acgccccgga aaatatgcag cgtcgcgggg caatgtattt gcaacgcgaa ttgaaagata
     2161 aagtcgcgat gatgaatcga ctacagcagg cgctggaaca caaccatttt ttcctgatgg
     2221 cccagccgat taccggtatg cgtggtgatg tttaccatga aattcttctg cgcatgaaag
     2281 gtgagaatga tgaactgatc ggccccgata gcttcttgcc ggtcgcgcac gaatttggtt
     2341 tatcgtcgag tatcgacatg tgggtcattg agcatacgct gcaatttatg gctgaaaaca
     2401 gagcgaagat gcccgctcac cgttttgcta ttaatctgtc tccaacctcg gtatgtcagg
     2461 ctcgttttcc tgttgaagtc agtcagttgc tggataaata tcagattgaa gcgtggcaac
     2521 ttatttttga agtcaccgaa agtaatgctc tgaccaatgt taagcaggcg caaatcacct
     2581 tgcagcatct tcaggaatta ggctgccaga ttgcgattga tgatttcggc accggctacg
     2641 ccagctatgc gcggcttaaa aatgtgaatg ccgatctgct taaaattgac ggcagtttta
     2701 tccgcaatat tgtgtcaaat agtctggatt atcagatagt ggcatcgatt tgccacctgg
     2761 cgcgaatgaa gaaaatgcgg gtagtggcag agtacgttga aaacgaagag atccgcgagg
     2821 cggtgctctc tttggggatc gattatatgc agggttatct tattggtaag ccgcaaccgt
     2881 taattgatac gctgaatgaa atcgaaccca ttcgcgaaag tgcctgaata atgcgggccg
     2941 acatttctcg tcggcccgca aagcattaag cggcgatttc aggtgtactt tcttcttcaa
     3001 ttttcaaccg ccagccagcc acgccttccc agtattcttg ctccttttcc agatcaagca
     3061 gtaccagcgc attctgacta aaccagtcat gcgggaaacg cagtgtccag tgactgtcat
     3121 cagtaatcag tgtcaatgtt ggcggtgtgg tggttgcctg acgttgattg tttagtaata
     3181 cgccaaggcg caatagctgt atcagtggca ggaactgttt cttcttaaac aaggtaaagc
     3241 gcggcagatc gtcgagctta atcgctttac ggtgatagcg caccagtgtc gccatcatca
     3301 gctgctgttc ctgattaaaa cccggcaagt cactgttttg cagaatataa gcggagtggc
     3361 gatgcaaacc gctgtggttg atattcaacc cgacctcatg cagcatggcg gcccatcgca
     3421 gtagcgcctc cagttgcgga tgcgccagct tcggttgctg ttcccgccac tgttcgtaca
     3481 tttgcatagt ggtatccagc actcgtcggg cctgttcgct gtcgatgtga tactggttgg
     3541 cgaggctgct ggcggtgcga ctacgcacat cctgatgacg gaaacgtcct tccatttcat
     3601 acagtacgcc ttcgcgtaac gccccgtcag aaaggcgcag ttcacggatg gctaaagcat
     3661 caaacacacc acataaaatc gccagtccag gaacgaagac tgttttccgc tcttcggaaa
     3721 gacccggtaa actcagcgat gcgaaattac ggtgacgtaa aacttctttt accagttttt
     3781 ccagacgttc cggggtaatt atcccgtctt tctcgcccat ttccattaat acttcatggg
     3841 cggcttttat ggtaccggaa gcgcccattg ccacgttcca gccctgaata cggaattgcc
     3901 aggttaaagt ttccagtttt tgtgctgccg ccatgcgagc gcgctgaaaa ttctctttat
     3961 tgatgacccc gccagggaaa taaagctggg caaagctgac acaacccatc cggcggcttt
     4021 caacgagaat aggttcgaaa ttttcaccaa tcaccagttc cgtagatccg ccgccaatat
     4081 caataaccag tttgcgacct ttttccggtt gggtatgttc cacgcccata aaaatcagac
     4141 gggcttcttc attaccggaa ataatttcaa tcgggtaggg aatgaccttt tccgcgcgtt
     4201 tcagaaagtc agtggcgttc agcgcctgac gcagtgtatg ggtgccaact atacagacgc
     4261 tggcaggaga aaacccttgt agccgttcgg caaacagcga cagacagttc aaaccgcgcg
     4321 tcattgcctc ttcactcaac atattatctg gccccaggcc gtccgccaga tgcacccgct
     4381 gtttcaggcg gccaataatc tgcatggcac catctaccac acgggctatg accatgtgaa
     4441 aactgtttga accaagatcg accgcagcaa actcctgcgg acgaggggat ttatcgtgta
     4501 ttggcatagg gttattcagg ttgttcgagt gatttgatgt agtcataaat cgccaactgc
     4561 gcccgtactt tgcggcgatt gccgcgggga acgtagcgat tactgagttc tttatcgata
     4621 taacgtgctt tgaccgtatc gctgaacaat atgtcgatga tgtccagcac ccgctgcttc
     4681 aggcgcggat cgagcagcgg tgtcgccact tcaatacgat aatcaatatt gcgcgtcatc
     4741 cagtcggcgg aagaaaggta gaccttttta tcgccgccat tttcaaaaat ataaacccgg
     4801 tcatgttcaa ggtaacggtc aacaatactg atggcacgaa tgttgtcgct aatgccttcc
     4861 agattgggga tcagcgaaca cattccgcga accagcagat taaccggtac gccggagctg
     4921 gaggccgcat acagacgatc aaccaggcct ttatcgacaa ggttatttag cttcagggtg
     4981 ataccactgg gcagcccttg ctgcacgttg gcgatttcgc ggtctaccat ttcatataac
     5041 agacggcgag agttttgcgg tgacaccatt aaataatcaa atgtcactgg gcggtatggg
     5101 ttttcaataa agttaaatac ccgccgtact tcgttggtga tacgcgcatc tgcggtcagc
     5161 aacgaatagt cagtataaag acgcgcggtt ttttcgttaa agttcccggt cccgatgtgt
     5221 gcgtaacgga ccacttcacc gttttcttta cgtgaaatca ggaacagttt ggcgtgaatt
     5281 ttcagccccg gcgcagagaa gataacgtgc acgcctgctt cggtcaggcg cttcgcccag
     5341 tgaatgttgg cttcttcgtc gaaacgcgcc tgtaactcaa ccaccacagt gactttctta
     5401 ccgttatgtg cggcgtggat catcgagtcg atgatgcgtg aatctttcgc cacgcggtaa
     5461 atgttaattt taatcgccag tacgctcggg tcgaacgaag cctgacgcag cagttccagc
     5521 acatgctcaa aggtgtgata aggataatag agcaacacat cgcgttcgcg aatggcatca
     5581 aaaccattgc ggaactgggc tttatcaaac caaatgtggc gtaaacgcgg cagtggtttg
     5641 ttcaccagat tggctttgcc gacattgggg aaattaataa agtctttaaa attatgataa
     5701 cgaccgccgg ggacgatgga gtcgtagcgg gaaatagtca gtttttcgcg taacacttca
     5761 accagcgcat tgggcatatc gcgctgataa acaaaacgca ccggctcagc agttaaacgc
     5821 tgcttgagac tggaagacat caactccatc aggctggctt ccatctcatg cactaaatcg
     5881 tattcggcat cgcgtgtcat cttcattgaa taggcattca gcgcgtcata atcaaagaag
     5941 cctttgaaaa tatcatcaag gcagtaacgc agaatgttat ccagaagaat catcggcttg
     6001 cgtcgacgcg gcgcttctgg cggtaaattc acaaagcgcg gcactttatc tgatgggatc
     6061 tccagcagcg cgtaacggat ggtatcacca cggataattt ccaccgccag ataggtgtaa
     6121 tcatccttca ggaactgcac taagtcagtg tcaggattga ttaaaatcgg cgtaatgtgc
     6181 tgacgcagat actgcttaaa ataatgacga agccagtttt gttgattgac ggagagctgg
     6241 cgttcattaa tcaggaagat ctggttgcgc gccatctcca gcaatagctc gttgtagagg
     6301 ccgtcgaatt cctgatcggc tttcagcacc cgggactgaa ttttgcccag taaatggcgg
     6361 gaatgagagt tggagccttg ttcttcgcta ataatgatgc gtcgcttcag ttcagcgaag
     6421 cggactttat agaactcatc aaggttattg gaatagatcc ccaggaaacg catcctttca
     6481 atcagcgggt tagatttgtc cgccgcttcc tgaagcacgc gttcattgaa cgataaccaa
     6541 ctgagctctt tttcgatgta tagcttttcc tgacccatta ccacttttac tccgttttat
     6601 tcacgggaca ctgcctggat attatggcga gcaatattgg cagatgtcca actatgccag
     6661 aaaaggggga aaaaaactgc tggagcttgc gatttacagg tgaattgaaa aacgccagcg
     6721 gcagagctgg cgctttaact acggggcatt actcgtcggc agcgtagccc tgcggcggca
     6781 gacgttgacc atccagccac gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc
     6841 agctaatcac cagtggataa atagcgtgtt cctgggtttg cacgcgggcg gtgatgtcat
     6901 cttccgtatc accagcaaat accgggactt tcgcctgtaa aataaccggg ccaccgtcca
     6961 gttcatcggt gacgaaatgc accgatgtac cgtgctcttc atcaccattt tccagcgctt
     7021 gacgatgggt gtgtaatccg ggatatttcg gcagcagaga agggtgaatg ttcagcaaac
     7081 gcccggcata gtgggagaca aacgccgggc tgagaatgcg cataaaacca gccagcacga
     7141 ccacatcggg tgcgtacatg tcgatttcat gaatcaactc ccggtcatag gcttcacgac
     7201 tgtcaaacgc gctggcgatg agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa
     7261 ggccgaacgc gtcggcctta ttgctgaaaa ctgcccgtac ggtgccttta attttgttgg
     7321 ttttacaggc gtcaataatt gcctgtaaat tacttccgtt accggaaata agcaccacaa
     7381 tattcattat tcgataacca cgcgttgttc ggaatcagag gctttgatga taccgatttt
     7441 ccacgcgttt tcaccgttgg cattgagcag ggcgagggct ttgtccactt ccggagcagg
     7501 cagggcaata atcatcccga cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac
     7561 gttacctgcc gtttgcagcc agttgaacac ttccggccac tgccaggaag attcatcaat
     7621 cactgcctga gtattatctg gcaatacgcg tggaatgttt tcccagaagc cgccgccggt
     7681 caggtgcgca atggcatgca catcgacctt ttcaatcaac tccagcactg acttcacgta
     7741 aatgcgggtc ggtgccagca gataatcggc taatggctta ccatcaagtt gggtggtttg
     7801 cggatcacaa ccgctgactt caagaatttt gcgcaccagc gaatagccgt tcgagtgcgg
     7861 accgctggaa ccgagtgcaa tcagcacatc gccgtcgctg actttagagc cgtcgatgat
     7921 ttctgatttt tctaccacgc caacgcagaa acccgcgaca tcgtaatcct caccgtgata
     7981 catccccggc atttctgccg tttcgccacc caccagtgaa cagcccgatt gcagacatcc
     8041 ttccgcgata ccgctgatca ccgctgaagc ggtatcaaca tccagttttc cggttgcgta
     8101 atagtcgagg aaaaacagcg gctctgcacc ttgcaccacc aggtcattaa cgcacatggc
     8161 gaccagatca ataccaatgg tgtcgtgacg ttttaagtcc attgccagac gcagcttggt
     8221 acctacgccg tcagtgccgg aaaccagcac gggttcacga tatttttgcg gcaatgcaca
     8281 cagcgcaccg aagccgccca gaccgcccat cacttccgga cgacgcgttt tcttcactac
     8341 gcctttgatt cttccaacca gagcattacc cgcgtcaata tcaacaccgg catctttgta
     8401 gctaagagag gttttatcgg tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa
     8461 taaaattcgg cgcaattcta acagggaaag caaacgtttg cgagactgct ttacacaacc
     8521 tttttgcacg tcttttgccc aggcgcgcgg cgaaagaaga cttgtaccag ggtaaaggtt
     8581 agttttcgga tggaataatc ttctttcata accatttgaa tataaaataa ctttatctca
     8641 agccgttatc attttgacta aagtcaacga aaagaatatt gccgccttga agaaaggagg
     8701 tataatccgt cgattttttt tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg
     8761 gaagtcaaac acccactcgt caaacacaag ctgggactga tgcgtgagca agatatcagc
     8821 accaagcgct ttcgcgaact cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc
     8881 gccgacctcg aaacggaaaa agtaactatc gaaggctgga acggcccggt agaaatcgac
     8941 cagatcaaag gtaagaaaat taccgttgtg ccaattctgc gtgccggtct ggggatgatg
     9001 gacggtgtgc tggaaaatgt tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat
     9061 gaagaaacgc tggagccggt accgtacttc cagaaactgg tttctaacat cgatgagcgt
     9121 atggcgctga tcgttgaccc aatgctggca accggtggtt ccgttatcgc gaccatcgac
     9181 ctgctgaaaa aagcgggctg cagcagcatc aaagttctgg tgctggtagc tgcgccagaa
     9241 ggtatcgctg cgctggaaaa agcgcacccg gacgtcgaac tgtataccgc atcgattgat
     9301 cagggactga acgagcacgg atacattatt ccgggcctcg gcgatgccgg tgacaaaatc
     9361 tttggtacga aataaagaat aaaaataatt aaagccgact ttaagagtcg gctttttttt
     9421 gagtaaagcg cctataacac ataatacaga ggataatact atgacgcgcc gtgctatcgg
     9481 ggtgagtgaa agaccgccac ttttacagac aatcccgctt agtttgcaac atttgttcgc
     9541 catgtttggt gcaaccgtcc tggtgcccgt cttatttcat attaacccgg cgactgtact
     9601 gttatttaac ggtattggaa cgctgctgta tctcttcatc tgtaaaggga aaattccggc
     9661 ttatcttggt tccagctttg cctttatttc accggtattg ttactgttgc cgttagggta
     9721 tgaagtcgcg ctgggcggct ttattatgtg cggcgtgctg ttctgcctgg tttcttttat
     9781 cgtgaagaaa gcggggaccg gctggctgga cgtgctgttt ccacctgcgg caatgggcgc
     9841 aatcgttgcc gtcatcggtc tggagctggc gggcgtagct gccggtatgg cgggtttact
     9901 cccggctgaa gggcaaacgc cagactccaa aaccatcatc atctctatta ccaccctggc
     9961 ggtcacggtt ttaggttccg tgctgtttcg tggtttcctg gcaattatcc cgattttaat
    10021 tggcgtgctg gtggggtacg cgctctcttt cgcaatggga attgtcgata ccacgccgat
    10081 tattaatgct cactggtttg cgctgccaac cctctatacg ccgcgcttcg agtggtttgc
    10141 cattctgact attctgccag cggcgttagt ggttattgcc gaacacgtag ggcacctggt
    10201 agtaacggct aatatcgtca aaaaagatct gctgcgcgat ccaggtctgc accgttcgat
    10261 gtttgctaat ggcttgtcga ccgtgatttc cggcttcttt ggctctacgc caaataccac
    10321 ttacggagaa aacattggcg tgatggcgat cacccgtgtt tacagtacct gggttatcgg
    10381 cggggcggcg attttcgcta tcctgctttc ctgcgtcggt aaactggctg ccgctatcca
    10441 gatgatccca ttgccggtga tgggcggcgt ttcgctgctg ctttatggtg tcatcggtgc
    10501 ttccggtatt cgtgttttga tcgaatcgaa agtggattac aacaaagcac agaacctgat
    10561 cctgacttcc gtgattttga tcatcggcgt cagtggcgcg aaggtaaaca tcggcgcggc
    10621 ggagttaaaa ggtatggcgc tggcgaccat cgtcggtatc ggcttaagtt tgatcttcaa
    10681 actgatcagt gtgctgcgcc ctgaagaagt ggtgctggac gcagaagacg cggatataac
    10741 agacaaataa tgtaccgacc gggcagtgtt gctgcccggt tcaaacatca tgggattctg
    10801 tggtaaactt ctcgcgattt tgtgaaatcc tggttgaggt atctctgaac acaccggcac
    10861 agctctcttt gccactttat cttcctgacg acgaaacctt tgcaagtttc tggccggggg
    10921 ataactcctc tttactggct gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca
    10981 tctatctctg ggcacgcgaa ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg
    11041 aattgtcgca gcgtggcgat gcggtgggct atgtcccgct ggataaacgc acctggtttg
    11101 ttccggaagt gctcgacggt atggagcatt tgtcgctggt ctgtatcgac aacattgagt
    11161 gtattgcagg cgatgagttg tgggagatgg cgattttcga tctctacaat cgaattctgg
    11221 aatcgggcaa aacacgattg ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg
    11281 gattaccgga tctcgcgtcg cgactcgact gggggcagat ctacaaattg cagccacttt
    11341 ctgatgaaga taagttgcag gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc
    11401 cggaagatgt ggggcgtttc ttgctgaagc ggctcgacag agaaatgcgc acgctattta
    11461 tgacgttgga tcagttggat cgtgcgtcga ttaccgcgca acgtaagctg accattccgt
    11521 ttgtgaaaga aattctgaag ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc
    11581 cttatccgaa ctacgcgaag ttaatccata gtattcgtag gccggataag acgtggcaag
    11641 cgtcgcatcc ggcaatgtgc ttcaactagc gatggagcac atcttaaccc acgatttcca
    11701 gtacctgctc cggtggacga ccaatccgcg ctttgccatt cgcgaccaca atcgggcgct
    11761 ccattagctt cggattatcc accattgcct gaatcagcgc ctcttcgcta agcgaactgt
    11821 cagccaggtt cagttcttta taaagatcct ctttctggcg catcaattca cgggcgctat
    11881 tcatccccag cattttcagc aaatcgcgca gcgttgccgc atcggcgggt gtctcaaggt
    11941 agagtaccac ttctggctcc acgccgtttt ctttcagcag gttcagcgtt tcccggctct
    12001 ttgaacagcg tgggttatgg tagattttta cctgtttggt cattgttctt cctttaatgc
    12061 gaattacatc ttggtataag gcttaaagcg ttcctgcagc tggcgcaact ggtcgatgcg
    12121 cgcatcgtaa cgcgcttgtt gcaggctgcc taatttcacc tgcgaactgg cgctactcaa
    12181 cagcgaaatg gcctgatcga gtcgtccggc gagcgcataa ccttctgctc gcgcagccag
    12241 ctcctgatcg cggttattta gcgcggcttc cgcctgtgcc agtaaatccc agccgttgct
    12301 gtcatcttta ttattaaagg tgtagcgatt cagaatattg gccgcttctt gtggttgacc
    12361 gccttgtaga taagcgttcg ccaggttgag ctgcaacacc ggattggtgc gcaaatcacg
    12421 ggcatttttc aggcgattga ttgcctcatt ggctttgttt tgcccaagat cgatatcggt
    12481 agccagatcg agataccatg cgttgccagg ttctgccgcc agtaacggtt gcagcgtttt
    12541 acgcgcctcg tcgtatttat tggcttccat cgcctgtaaa gcacgaccat attgcgccgc
    12601 tcgttgctga cgaacgtttc ctttcgccca ttcatccagc aaatcactgg tgagctggtt
    12661 acgtccggaa ttatacatcc ccagtgtgcg cgctttcgcc agatagaaat cttccgacga
    12721 ctgcaccacc atcggtcgca tctgattagc acggttgcgg gcatctgcca gacgactttc
    12781 cggcaacggg tgagtcaata aaatttccgg cgggcgcgag gagtaacgcg cctgatcgag
    12841 taatttttcg aggaaggttg gcatcgcctg cggatcgaat cccgagcgtt gcagcacctg
    12901 aataccaatg cggtccgctt cctgttcatt ttgctgggtg aaactgatca ttccctgacg
    12961 tgttcccgcc agtgtaccgg tcagcgccgc catccccgcc tgcggactgg ccatcgccag
    13021 taaaatagaa cctaacgcgc cgacccaggt cagcggcgcg ctgcgttgtt gatcttccat
    13081 cgctcgcgcc aggtgacgtt gggtgacgtg ggagatttcg tgcgccataa ctgaagccag
    13141 ttgactttcg ttatcggaat aacggaacag ggcagagtgc agcaccacgt tgccgccaaa
    13201 gaaagcaaag gcgttaattt cgtcgttgtt gatcagaaaa aaatgaaacg gtgtcttaac
    13261 cgaattggca tgcgaaacca gacgcatccc cagcgaatta atatattgcg ttaacagcgg
    13321 gtcattaatt aacggcgcgc tgccgcgtag ctggcggaca taatagtcgc ccatctgcat
    13381 ttcctgacca atggaaagcg tgcttcctgc ggaggttccc atatccggca aggtgtctgc
    13441 gctgtcggca aacgccgggg ctacctgacc aatggtcata gcagcaatga gggttgcaac
    13501 caggtttttt ttcaactgcc tgaacataac ctctatcctg tatttctgaa gatagtcatt
    13561 tgaccgatac tgttaaatat cgttcccacc gcgccgcctt tgagtgtaac ggcgaagatt
    13621 agcccactca aatgactttt cagtttcctg aatccgtcat aaaaagcgaa gtcttttttg
    13681 tgacatttcg atacaattgc agatcgcaat cccgctcttg aggttcaggg aaggttttta
    13741 tgctcgaaat gttgatgcaa tggtatcgcc gccgttttag cgacccggaa gcgattgcct
    13801 tgctggttat tttagttgcc ggatttggca ttatcttttt ctttagtggc ctgcttgctc
    13861 cgttgctggt ggctattgtg ctggcctatt tgctggaatg gccaaccgtg cgcctgcaat
    13921 ctattggctg ctcccgccgc tgggcgacgt cgattgtatt ggtggttttt gtcggtatat
    13981 tgctactgat ggcgttcgtg gtactgccta tcgcctggca acagggcatc tacttaatcc
    14041 gcgatatgcc ggggatgctc aataagcttt ctgactttgc cgccacgttg ccgcgccgct
    14101 atccggcgtt aatggacgcg ggcattattg atgcaatggc cgaaaatatg cgcagtcgga
    14161 tgctgaccat gggcgattcg gtggtgaaaa tttccctcgc ctcgctggtc ggtttgctga
    14221 ccattgccgt ctatctggtg ctggtgccat tgatggtctt cttcctgctg aaagacaaag
    14281 agcagatgct gaacgccgtt cgtcgggtgc tgccgcgtaa ccgtgggctg gcaggacagg
    14341 tgtggaagga gatgaatcaa caaatcacca actatatccg cggcaaagtg ctggagatga
    14401 tcgtggtagg gatcgccacc tggctggggt tcttgctctt cgggctgaac tattcgctgc
    14461 tgctggcggt gctggtcggc ttctcggttc ttattccgta cattggcgca tttgtggtga
    14521 ccattccggt ggttggcgtg gcgctattcc agtttggcgc aggcacggaa ttctggagct
    14581 gcttcgcggt gtatctgatt attcaggcgc tggacggcaa cctgctagta ccggtgttgt
    14641 tctccgaagc ggttaacctg catccgctgg tgattatttt atcggtggtg atcttcggtg
    14701 gtttgtgggg attctggggc gtattcttcg ccattccatt ggcgacgctg atcaaagccg
    14761 tgatccacgc ctggcccgat gggcaaatcg cgcaagaata atcttcagcc cgacgaactg
    14821 tttcgccggg ctgatttttc aggattcctg acgtaaataa atagcccgat aacacatact
    14881 caccagcact gcaccgccga taatattacc cagcatcacc gggagcagat tggcggtaat
    14941 aaaatggctg accgtcagtg ccggaaaatt gtctgcgcta ctgtgcgcca gttgccagaa
    15001 ggaggtaggg gcgaaatggc gaatggcaat ggcgaacgga atcacaaaca aattggcgat
    15061 acagtgctca aagccactgg cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt
    15121 gtcgcataac gaacgcccgc aataactcat ccacagcgcc aggcaaacca tcagattgca
    15181 cataatgccg aggctgacag attcagtaaa tgtatgatgc attttgccct cggcgcagtg
    15241 taaaaccgcg actccccaca tcgcgttttc actcatcacc agcccggaaa accagattaa
    15301 caaactgaaa caggcaatac ctgccagatt accgcaggcc accagaagtg cgttaatcag
    15361 ccaagttcgc caactaataa cgcccctact ttttgccatt accgtcatta ccgacgaggt
    15421 gaacaggttg gtgccgcaaa ccgccagcaa aataaagcca agtgtaaagc aaaggccgcc
    15481 caccagatgg gttaatgcct gtgaagacgg ggcatcggcg ataactgaaa ggtaaaaagt
    15541 aaatccgatc gccataaaca cgccagcgga catcgccatt aaaaagaccg acactttact
    15601 gcgggcaatt ttatgggcgg caatccgttc agcgattgcc gcttttctgg cgctcaactg
    15661 caagtcgaaa gagagtttgt ttctcatgca tactcctgca aaagcagatt acaacacctc
    15721 gcgaaccgag attcccagac gctgcattcg tgacagcagc gtggtgcgct tcatccctaa
    15781 tcgtgtcgcc gcgccacggg gcccggcaac aatgccattg gtttctcgca atacctgaac
    15841 aatgcgctga cgttcttctt cgtcattctc tggcgtcgtc gggtgcagca actgcgccat
    15901 tgaactgcga agcgctgaat cttcatttag cgtcggcagt aaacggcttt gtcggacatt
    15961 aagatgtaaa ttcagactgt taccgcgagt cagcagtacc gctcgctcaa tcacgttttc
    16021 cagctcgcgc acgttgcccg gccagtccca cgacatcaac tggcgtagcg cctcggtcgg
    16081 gatggcgtca attgaacgat tcatatggcg cgccattttt tgcgtgaaat gctttgctaa
    16141 aagagggata tcttccggac ggtcgcgcag cggcggcaat tccagtggga agacattcag
    16201 gcgataaaag agatcgctgc gaaactggcg atcttcaacc atttgccaca aatcacggtt
    16261 ggtggcggca atgactctga catttaccgg gatcgttcta ctcccgccga gacgctcaat
    16321 ctcccgttcc tgcaatacgc gcagcagttt aggctgaagt tctaacggca gatcgccaat
    16381 ttcatcgaga aacaacgtgc cgccatcggc aatttcaaaa cgaccacgat gggtattaat
    16441 cgcaccagta aacgcccctt tgtcatgacc gaataactca ctttccagaa gactggcggg
    16501 gatggcagcg cagttgattt tgaccagcgg cttgtcgcgt cgcgggctaa gttgatggat
    16561 cgctctggcg atcacctctt tgccggttcc ggtttcaccg caaatcaaca ccgtactgtc
    16621 gctcttcgcc acaatatcta cctgctggag taggtcttcc attgcctggc tttgatagat
    16681 aatgtcaccg atgcccagat tcgaaaggag ctgctcgcta agctggtggt tttcttgctg
    16741 caaactttcc tgcaaatcgg tcatgctacg ccaggcatcg gcattgccaa cggcaatagc
    16801 gatgcgatcg gctatgtgtt gtagtagctg gcagttttcc tcactaaaga gagtggaaga
    16861 ggtatgcgcc agcaacaatg cacccggtgt atggttgcca aaggtaagcg gtataaggag
    16921 cgcagattcg cagccgttag atgcaagtaa gagtaataac gggtcgcgtt gccagagaaa
    16981 cagatcgtct gcacggtgcg tcaaggtcgg ttgtcgggtt tgtagcgttt gtgtcagcaa
    17041 tacactgtcg ccaggcatat tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct
    17101 gcaccacagg ctaaacttct cgttctttcg atgatcgccc agtaccatac tgactgaagc
    17161 cagaccgaaa aaatgatgga tctcacgagc gacgtcagcg atcagatcgt cgagatcaag
    17221 atgtgacagc actgcattag taatatcaac cagtacccga tgatgatcgc gatctttctc
    17281 cagcaaacgc agcgccacct gctgttcttc atcattgagc catgattgca gaaccggggt
    17341 gattatccgc gccagccagt taaacagtgt gcaatcctgg ggagtaaaac ggctgccgtg
    17401 ggtacgaata aagcgtagct ccgccattcc actactgccg agaaggacgg cgtgacgcga
    17461 ataatcgacc atctcgccct gatactctgt tgttgccgtc acatcgctgc catcatcatg
    17521 gtagcggtaa tagaagttgc ttttgagaac atgcagttca ataagttgta ccgggctgaa
    17581 cggcggcggc atcgggatga aggcgcgtaa taaagacgcc ttaccgtgtt tctgtgctaa
    17641 ccgctccgcg agcattccgt ttaccgcttc aattgttatt ccttgtggcg gggcaaacat
    17701 cgcctcgtct gacatagcca tcaacagtcc ttattaatga gaaatccttc tcacgcaatt
    17761 tttctggcca tcagatagat ggccggttcc tgcacaattt catccaacag atgaatgaat
    17821 tgaatgctac gcgcctgcca cacagacgtc gcgtcatccg ccagtggcgc gaatgcccgg
    17881 gtcaacaggg cggtgtggct ggcgtcaata acaatctcac caaaagtcat cacccccagc
    17941 atcttacgtc gggcttgctc ctcttcgacc tgggcaagcc aatcttcgta ttccgtcagt
    18001 gggcagggga aggcgacatt cagacaatca atcacgccaa cgtgatgacc gatagccagc
    18061 gagtaataca ttacctgaga gctgtgttcc ggcatctcgt cgctactggc gacaaacttt
    18121 tttcgcagcg tccagaaaac aatttccccg cactcttcag tcatgtaacc cccggagtac
    18181 gcgaaagagt tgctgaacga tactattgag acgtggatcg tcggcatcgc gcaaccaggt
    18241 gttaacccga tttcctgtag gatcgtcgct taaatggcgc aggagccgat cgcaaatttc
    18301 tcgcccctga cgatagcccg ccagccgccg cgcttctcgc tcaatggcga tacgctgtga
    18361 tggcgggatc tgcggccaca acggttgcat agtcaccccg gtgggatcgc gataatccac
    18421 cgcgtgaatc ttctgttgca gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc
    18481 cggtgttggc gggcagccgg ggatccaaac atcaatgggg acaatggtgt cgctgccgcc
    18541 ccagacgctg tagagatcgt ggaaaatccg ccgccggcac cgcacgcgcc gtaggaaaca
    18601 cagattttat gatcgggggc agactcatac gcccgaagtg caggcatacg catcgcccgg
    18661 gtgactgcgc ctgtaaataa caaaatatcg gcgtgacgcg gtgatgaaat aaccttaatg
    18721 ccaaaacgtt ctgcgtcgaa tactggtgtg atggcggcaa aaatttcgat ttcacaggcg
    18781 ttgcagccgc cgcaatcgac gcgataaacg taagccgagc gacggatatc ctgtagcaaa
    18841 tgctgcttca tcttttgcgt ttgctcgtcc agcgtgatgg gctggctgac atgttgtgta
    18901 agcactggac tcatagctgc tccttagcca ccagcggtac atctgtatcg tcgttgagca
    18961 gcgtcgcgcg ttgtttgcac tccgggcaga cgctcgcttg cgcccataac atttcgcggt
    19021 tttgtggcgc attttgttgc tgtgccaaca attcagcagc cagtgcgacg gttttttgcg
    19081 gggcaaacgg gcgttcgcaa cggctgcaac gttgtagatg gaacgtcgcg cgggtataga
    19141 gatcggcttt attggtgacg gtcagttcaa agttattggt aagctggatg gctctggtcg
    19201 ggcacacttc ttcacaacgt ccgcagtaaa tacaacgcct cagatagagc tgccaggtgc
    19261 gcgaattttg ctggtcgtcg gtctggatag tcagcgcatt tgccggacaa gcacaggcgc
    19321 aggcaccgca ggcaatacat tggctgggca tcaggtccgg tttcccgcga aagcctgggc
    19381 tgacctccag tggcgcgaag ggatatttca ccgtcgcggt tccggcgcgc ataatagttt
    19441 tcagtaactt cagcatcttc tgtccttact tcagcggcga acggttacga tcaataccgt
    19501 agcgttcgat ctctttatac ggcacggttt ttgactggcg cttgcgcaca tctaccagcg
    19561 tcacacggtc ggtacaggag tagcagggat caaggctacc gataatcagc ggtgcgtcag
    19621 aaacggtatt gccgcgcagc atgtaacgca acaccggcca gttggcgtag gtggcggcac
    19681 ggcagcgcca gcggaacaat ttttggttat caccgagcat gctccagtgc acgtcttcac
    19741 cgcgtggcgc ttcaacaaag cccagcgcga atgcgtgagg tttatagcta aagccttcgg
    19801 tcagcagtgg ggtatccggc atgttgtcga gggcaaattc cagcattgcc agcgaatcaa
    19861 acgtctcttt gacacggacc atcacgcggg agaaaacatc gccgccggta aaggtaaaca
    19921 gtgtttttgg aatattgccg tagtcggcgt aggggtgatc aaagcgcaaa tcacgggcga
    19981 tttgtcggtc gagaatgcca atgccctgag tgcgttgttc catattcggc gtagcaagca
    20041 gcatctctac cagctccgac acgtcggcgc gcatctcgcg caccagtttc agcgtttgca
    20101 gacgttgctc tttgagaata tcgcggcgaa caccaccaat cagattcaga ccgtaggttt
    20161 tacgcgaccc gatcagcaat tccgccatcg tcatcgactt ttcccgcacg cggaaaaatt
    20221 gcataaagcc ggtatcaaaa ccaacgaaat ggcaggagag gccaaggtta agcaaatgac
    20281 tgtgtagccg ttcgacttcc agcagaatcg agcgaatagt atgtgctcgt tgcggcacct
    20341 caatccccag tgcattttca accgagttgg tataggccac actgtgggca aaaccgcaaa
    20401 tcccacacac gcggtccgat aagaaggtca cttcgttgta gcccatccgc gtttctgcca
    20461 gtttctccat gccgcgatgg acataaaaca ggcggtaatc agcatcgaca atttgctcgc
    20521 catccacaaa caagcggaag tgacccagtt catcggaggt gatatgcagc gggccgacag
    20581 ggatcacctg cgcatcgctg ttgccctcat tgataaacgg atacgtttcg gaatcagtcg
    20641 tcggttcagg gcgcagtcga taatccatcg catctttgcg cagcggatgc atatcttccg
    20701 gccagtcatc gggcaacacc aggcgacgat gatccggcag gccaaccgga atcagcccgt
    20761 acatatcgcg aatttctcgc tcgccccaga ccgcggcagg gacgcgcggt gtgactgacg
    20821 gaaactcccg actgtcggca tcgaccagtg ccttcaccac gatccagcat ttttcggccc
    20881 cttccattga aagggcataa taaaccgcgt aatgaccgtt aagtgtccgc tcgtcgttgc
    20941 caaacaggac tggaagccag ccatcatgtt gataataaag ataatgtaca acgtcaggca
    21001 gcagattgat tttcaccgta atggtgacct gttcaggcgt ttgtcgctct tcatccagca
    21061 ccgcgccggg gaactgcgtt ttcagggcgg cgagaatcgc ttcgccacga tttgacgatg
    21121 aattaacgtt cactgtgttt ctccgaaaca gatgcggtgc ctgaaggcaa aaaatcatgc
    21181 caggtgctac gttgtgcagg caggtcgtgc gtccctgaga ggactatagt ggaagcgccc
    21241 gccaggatcc tgatgacagg ttgtggaata tgcgttccca tcgccagcat catgaccagc
    21301 agaatcacca ttggcgaggt ggtcaaccag ccgagatcac cccggttaac ggcctgcggc
    21361 ggtttcgcca ttaacacccg cgctgccatc cgtaccaggc ccgccagcac cagcgttaac
    21421 agcaataaca gcaggacgat aatcagcagg tgattacgtg ccagtccggc ggtaatggtc
    21481 ataaattcgc taagaaaaat gttgaagggc ggcatccctg ccagcgccag cgcaccgccg
    21541 ccaaacagca cggcggtaaa tggcatgatt ttgagcatcc cacagacgac gttgagatcg
    21601 cgcgtgccgt acttgagcag tacattgccg gaaccgcaga acagcagcgt ttttgccaga
    21661 ctgtggttta aggtgtgcag cagcgcggca aaaattccca gtggcccgcc aatgcctagc
    21721 gccaccgcga ccagtcccat gttctccacg ctggagtacg ccagcagacg cttaatgtcc
    21781 cgctgtacca gaatgaaaaa tgccgccacg gcaaccgaca acatgccgaa gatgagcaac
    21841 aaccggttgg ggaaatcgct gccgatggcc tggcagataa tgatgtaata gcgaattagc
    21901 accaacagcg cgcagttcag caatacggcg gagagcaggg cgctgaccgg actcggtgct
    21961 tcactgtgag catccggcag ccaggcgtgc atgggaaata gcccggtttt ggtgccaaag
    22021 ccaattagca caaacacaaa ggccaacagc attaatgttg ggtcaagcaa ggacgattgc
    22081 ttaagaacct cgctccagaa tatcgccatt tctgcctgcg gcataacgct ggcgacgttg
    22141 gcgtatacca gcacggtacc gaacagacca aaagcgacac caacagtaca aataatgatg
    22201 tacttccatg ctgcttccag cgatgaacgc tgaccgtaaa tgcctaccag aaacgccgag
    22261 cttaaggtgg tggcttcgat cgccgcccac atcacaatca ggttattgct ggtaacaacc
    22321 agcagcatgg taaacaaaaa cagatggaag aaaccgtagt aatcgcacag cgtaacgggg
    22381 gaaagctcgc cgtgtgccac ttcatgacgc atgtagccaa tcgagtaaac accggtgaga
    22441 aagccaatca caccaagaat ggcgaggaac aaaccgccca gaccatcaat atgaagccac
    22501 agtcccgcag cgaatatttc tcctgcatca gcggcagttt ggaccaccca gagtgccaga
    22561 atcagcagca gtgtgatccc taagctatgt aatacagtca ccgtacaagt cgcagagagc
    22621 ccccgtttcc ggcaggcaaa acagagcagc gaaaaaagca gcggcgtgag caggagtaaa
    22681 gcgaacatca cagaataact catctcatta tcccttcagc gcggtcaagt tgttcacgtc
    22741 cagcgtgccg tgggtacgcc atatttttct tgccagtaac accatcacaa tgacggcgaa
    22801 gatggcgtcg gtagcgatac ctatttccac cagttccggt gctcgccagg caagaagcgc
    22861 cagcaccaga tgggagccgt tttccatcag gcagtaacca aaaatttgcc gcaggatatt
    22921 gcgctggctg acaatgcaca gcaggccaag cagaaaatga cctaacgcta ccgccagcgc
    22981 cggtttcagc ccggtagcca tcggtagctt cacgggctga acgacaaatg cgcaaagcag
    23041 gacaattaac gccgcgagca gtgccatcat tgccggaccg aataacgctt tttccgggat
    23101 gttctgggga atatttcgtg cagcgtaagt catgattaac ggtaccagca gcactttggt
    23161 gataaaggcg ctggcggacc agatcagcag ttgctctgcg gcgaacaggc acgagagagt
    23221 ggcgaaaata gacaccagca ccagcgactg gcaggcgtaa aatccgcatg acaggcgata
    23281 gcttttgacg ctaatcacaa acagcgatgt cagcatcatc agtcccgcca gattatttac
    23341 gatcatagaa ccggtcattc atcatctcct tactgcaacc aggtcgcggc gattacgctg
    23401 gaaatcagcg acatcacaat cagtacaatc aacaccaggc gcattgaccc tggcagcggt
    23461 gcggcatcgg cgacggcctc gctcggtttg ccaggcacaa cgcgaccaaa ccagcgaata
    23521 aaccaggcga aactggcgac cgattcaatc atcagcagaa tcatggcggg cagcaggatc
    23581 cagtactcca ctgacaacgc aaaaccggca gcaaacagcg ggaatttact gaagaagccg
    23641 ttgaacggcg gcacgccggt aatcgccagc gctgccacgc agaaacccac gcctggcaac
    23701 ggcagggtgt gcaatacgcc acgcagacgc ggcaacaagc gcgtgccgca gctgtaactc
    23761 agcgcacctg ctacaaggaa aaacaggctt ttagcgaacg cgtggttgac gatgtaggcg
    23821 atgctgccct ccagcgccag ccgcgagccg aagatggaga gcgacaaacc gaagaacatc
    23881 cagccaagtt gagtgatggt cgaccaggcc agcaaccgct tcatatcctg ctgtggcaaa
    23941 tacatcagaa agccataaag aatggtgacc agcgccatga ccatgccaac gccgccaatc
    24001 acatgcggga tattgccgcc gtcgataatg gcgcgggcaa aaatgtaaac gcccactttc
    24061 accttcgatg cggcgtggag ataggcactg atcggtgttg gcgcttccat tgcatccggt
    24121 agccacgctt gcatcggtag ctgggccgat ttcccccacg cggcaaacag gatgccgcca
    24181 taaaccagat aacgtgcgtc gccgtgtaac tcgctcatcg cgctaagcgc aaacgttccg
    24241 gtctgcaaaa acagcgtggc ggcggcaaga tacaacccca acgagccgat atgagtgata
    24301 agtaacgctt ttagtgctga acgctgtgct ttatcgctct ggtaataact gatcaacgcc
    24361 caggagcagc cgcccgtaat ttcaaaaaac aacaactgac cgagcagcgt cgaggagagt
    24421 accagtcccg ccatcgcgcc gataaacaac agcaaaaatg cgtaataacg attcgtgccg
    24481 ttatgcgggt gttcgcgatt tttatccgtc agataacccg tggagtagat cgtgaccagc
    24541 aaaccgagaa acaccaccac aaacagaatc agcgtactca cgcgatcaat gaccaggcca
    24601 aacaatgcca catcccccac gttgaccaat gtcaacgtga cggcaacttt atcggcctga
    24661 taaaacgcgg agatcagtga caacatgcac agcgtggtca gcgcggcgaa caaaaccccc
    24721 cattcggcgg cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc
    24781 aataacgtcg tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa
    24841 ccagaagacc catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag
    24901 taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa
    24961 aaccttgagc agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc
    25021 gcgcccaaag gacagaaaca gggccacaaa cagtgatgcc atcacgacct gtttcagccc
    25081 cagcccccat ttcgctagcg ccagcccggc accggaatat tcggtcagcg ggccttcctg
    25141 taattcctgt tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc
    25201 aaaaccacag gccagtaacg ccagtacggt ggttagcggc gagttccagc ccatcgccag
    25261 cgtattgctg atcatctcga tatgcgtgga acctgctatc agcgccaata ccagcagtga
    25321 gagaataagc attggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc
    25381 cgcaaacgga cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag
    25441 aagatagata agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaacag
    25501 tggtaaggcc atcgccagca ccagcatgct gctgattaat acccacggca tcaggcggaa
    25561 catcagacct gaagatgtcg gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg
    25621 ataatcctgc cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta tctgccggga
    25681 aatacccgta aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat
    25741 aaccagatat ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga
    25801 ttagcagcac aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt
    25861 gcagatgctg tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc
    25921 gctcatccca gaagggttct gtgctctgag ccctggcggt gacgtgcgca aaacctttat
    25981 ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa taacgcagag aagaccacac
    26041 gcagcggctg catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc
    26101 atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt
    26161 aaaggccagg cagcaaaggc atcgccagta gtaacagcaa cagtaatgag ggggtgacct
    26221 gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc gacagtgacg ggaggggtat
    26281 tggtaaacgc atgggcaata tgcattatct tcggtgccag ccaactggca ctaagcgcaa
    26341 ttaatacgca gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct
    26401 gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa agtaataccg tacattttta
    26461 cgaagcacat tactgccagc ccaccagtga ctgccagcat taccatagca ataggacccg
    26521 caagttgtag cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc
    26581 attcgctgat aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc
    26641 atgctgcggc tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg
    26701 tatgcaaacg cgaaataatc gcgcccgcgc cgagaaatag cagccctttg aacagcgcat
    26761 ggttgagcag atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgca
    26821 gtgacagacc gaccatcgcc acaccgactg ccagcaaaat aatgccgacg ttttcgacgg
    26881 tactccaggc cagcagccgt ttgatatcct gttccgccag cgcatacagc acgcccagga
    26941 gcgcggagat tgcgccgatc gccatcacca gaatgcccca ccacagcggc aaacccgttt
    27001 gcgccagcag gtccatcgct actttcagga tgccgaaaat accgattttg accattacgc
    27061 cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc aaccagctgt
    27121 gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa ggccagcagg aacaccgccg
    27181 acgccagccc cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg ctgccgcttt
    27241 cgcgccccat cagcaagaag gcgatcataa tcagcaccga tccggcgtgg gcgataaaaa
    27301 agtagagcat gccagcgttg atcgacgttt tatcctgcct ggcaatgacc agaaaccagg
    27361 aagacagcga catcatttca aacagcacga tgaaccaaaa agcgttgtcc atcaccagca
    27421 gggcaaccat cgatgcgatg aaaagattca taaagaagcc catcgccgcc gcgcctttgc
    27481 cctcgtattc gcgcatataa gtcaatgaat agagcgaaca aaccaccacc agcaaggaga
    27541 taaccagcac cataaatgcc gagaggctat ccattcgcag ggtaaaatcg gcaaagtcaa
    27601 aaggggtggc aaaacgggcg actaaagtta caccagcatg taattgcgta attccgctga
    27661 tgatgccaat cacgccgccc accagcgatg tgatgccgga aagcttaata gccagtctgt
    27721 ccagaccgag taaaaacagc gaagccagac tggcaaacag atagaggatc agcgaccagg
    27781 ttaataattg cagggcatcc attagcgttg ctcctgagtg agggaaaggg gatcctcccc
    27841 gccattggca aaccagcttg cggcaaggcg ctgtttttct ttcatctgtc gttgcaggct
    27901 atcgtcggtg atcagccgta acgcctgatt cgggcaagcg cgaacgcagg ccggaccttc
    27961 tggcaagaaa tcacacaggt cgcatttcac cgcgacggtc tggacacccg gttcccagcg
    28021 cagcaatgca tgttggggcg gcacgttttc ttcgccgtct ttgagtgagc cttcagcctg
    28081 aaaaacatat tgcgcagggg cattcaccgg acggcttcct gaagcgctga ttgcgccaaa
    28141 tgggcagacc acggcgcaaa gcttgcagcc aatacagagg ctttcgttga gttggatcgc
    28201 atcatccctc tgggagatgg cattaaccgg gcagacctgc aggcaagggg cttcctcaca
    28261 gtgatgacac acgacagggg cagtgagtgt tgacgtcttc gccagggcca ggcgcgggtg
    28321 ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg tattacatcc
    28381 tgtacaccac agtggttcgg ccaccacaaa gcggttcatg tcggctcctt gaaaatgata
    28441 tgaaacattg catttcatat tgcataaagg atgccattct gattaactca atatattcaa
    28501 taagttatgt attgactgga ttctgaacgg tacaaaaatg atggtcatta acgttcagca
    28561 aaaaatggat tcgacacccg tgtcgcaggg gaaagcgaga atgtgatctt gatcacgaga
    28621 agtgaggtat gctggccgca agcgcagcca gcacggaatg gagcaaagta atcaggcgtg
    28681 ttctttcagc cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac
    28741 atgttcgatt ttgccatcag cgtcaatcag gaagctgatg cgatgaatgc catcgtaggt
    28801 tttgcccatg aaggactttt caccccagac gccgaattgt tcgcacacct ggtggtcctc
    28861 atcagacagg agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg
    28921 tttatcggtg ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc
    28981 gcgtaagccg caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac
    29041 cagaacacgc tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa
    29101 gctaaatttc ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt
    29161 tcatcatgtt gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat
    29221 agggctttga acgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcggtt
    29281 atctgaatat gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg cgacaccagc
    29341 tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg
    29401 gagtctgcca catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc
    29461 gtcgtgcgct tcatcacgat taaaagatcc agttcggcac ctttcaacgg taacgttgat
    29521 tcaatcagag tgatggcatt ccatgaaccg gaaagcagca tgataaacgt gaactcttct
    29581 cccagcatcg ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg
    29641 atggtgttca caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc
    29701 gatgacagtg tcaaactggt tattccttta aggggtgagt tgttcttaag gaaagcataa
    29761 aaaaaacatg catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa
    29821 acgtaccatt gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc
    29881 gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg
    29941 attgattatc atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag
    30001 tccgctacct taaatcatga cgaacatgct gatgtggtga tgatgacgct ggatctggct
    30061 gatgggcgca ttccggtaat tgccgggacc ggcgctaacg ctactgcgga agccattagc
    30121 ctgacgcagc gcttcaatga cagtggtatc gtcggctgcc tgacggtaac cccttactac
    30181 aatcgtccgt cgcaagaagg tttgtatcag catttcaaag ccatcgctga gcatactgac
    30241 ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg
    30301 gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta
    30361 acgcgtgtaa accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat
    30421 gatgcgagcg cgctggactt catgcaattg ggcggtcatg gggttatttc cgttacggct
    30481 aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc
    30541 gaggcacgcg ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc
    30601 aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg
    30661 cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag
    30721 catgccggtt tgctgtaaag tttagggaga tttgatggct tactctgttc aaaagtcgcg
    30781 cctggcaaag gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc
    30841 acgctataag cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga
    30901 gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc ggtgattatg caatcccggt
    30961 gaccaacggt agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgct
    31021 ggcactggtt tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga
    31081 aaatggtcgt ggcaatactc tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta
    31141 caccatcacc caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa
    31201 ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg
    31261 ttatcagcag gcggttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc
    31321 agacgcggct tccatgcagc gttacagcac ggagatgatg aacgttattt ccgccggtct
    31381 ggataaatct gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga
    31441 cgtacaaagt gcagctgatg acaccggttt accaatgctg gtcgtacgcg ggccgttcaa
    31501 tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag
    31561 cacccgttcg cagggcaaca tggccgtaac ttataagccg ctgtctgaca gcgactggca
    31621 ggaactgggc gcgagcgatc caggcctggc atccggtgac tataaactgc aggttggcga
    31681 tttagataac cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag
    31741 tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc
    31801 tggaatcatc cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg gcaggaaaac
    31861 gcgattttag cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg ataaggcgtt
    31921 cacgccgcat ccgacaaaac atccggcaca ccagacagca aaagatttta aaacgttaat
    31981 tcacacccag gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa
    32041 accgtataca gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca
    32101 gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc
    32161 aattacttca ttatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg
    32221 ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg
    32281 cgtaaccgtg ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg
    32341 aacccgccgc tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac
    32401 gaatcttact gcgaaacctt tggctgggtg agcaaagaga acctggcgcg tatgaaagag
    32461 ctgacctaca aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctgattctg
    32521 gtcgacttca agctggagtt tggcctgtac aaaggtgaag tggtactggg cgatgagttc
    32581 tccccggacg gcagccgcct gtgggacaaa gaaacactgg agaaaatgga caaagaccgt
    32641 ttccgccaga gcctcggtgg cctgatcgaa gcctatgaag ccgtcgcccg ccgcctgggt
    32701 gtacagctgg actgattttt ctggttgctt gtttctttag ccggagggta ctccggctaa
    32761 atgtctttga tacccccgct acaatacttc tcattatctc cctttgacag gaaagtcata
    32821 ccaggccaat atgtatatac ataacgtata tcccaaaaag ggggggctat ggaaactact
    32881 gtatttttaa gtaatcgcag tcaggccgtc agattaccga aagcggtcgc tctgccagaa
    32941 gacgttaaga aagtagaaat tattgcgatt ggtcgtacaa gaatcatcac tcccgccggt
    33001 gagtcctggg acagttggtt tgatggcgaa aatgtgagtg cggattttat ggatatccgt
    33061 gatcaacccg caatgcagga aagggaatca ttttgatgct gaaattcatg ctggatacca
    33121 atatctgtat tttcactatt aagaataaac cagtacatgt cagagagcgg tttaacctga
    33181 attctggccg gatgtgtata agttctgtaa cattgatgga gcttatctat ggggcagaaa
    33241 aaagtcagat gccagaacgc aatttggcgg ttattgaagg tttcgtatct cgtttggtag
    33301 tgcttgatta tgatagtgct gctgcgacgc ataccgggca aatcagggcc gagcttgccc
    33361 ggcagggaag gcctgttgga ccttttgacc agatgatagc gggacatgct cgcagtcgcg
    33421 gtttgattgt ggttaccaac aatacccgag agtttgagcg agtggcggga atcagactag
    33481 aagactggag ctaacattct cttttacttc taacattcgt aaattatata acgtggcatg
    33541 aagccttaat tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac
    33601 ctacgatcat ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg
    33661 tgaaagtgac aatgttgaag acaggcgcaa cagctccggt ggtccgtcta tgggcggtcc
    33721 cggttttcgc ctgccaagcg gtaaaggcgg gttgatttta ctgttagtcg tgctggttgc
    33781 aggctactat ggtgttgatt taaccgggtt aatgactgga cagccggttt cccaacaaca
    33841 atcaacgcgg tccattagcc ctaatgaaga cgaagctgca aaattcacct cggtgattct
    33901 ggcaaccacg gaagacacct ggggacaaca gttcgagaag atgggtaaga cctatcagca
    33961 accgaagctg gtcatgtacc gtggaatgac gcgcaccggc tgcggggctg gtcagtccat
    34021 tatggggccg ttctattgcc cggcggatgg cacagtttat atcgatctct ccttctatga
    34081 tgacatgaaa gacaaacttg gcgcggatgg cgattttgcc caggggtacg ttatcgccca
    34141 tgaagtcggt catcatgtgc agaaactgtt aggcatcgag ccgaaagttc gtcaactgca
    34201 acaaaacgcg acgcaggcgg aagtgaaccg cttatctgtg cgtatggaac tccaggccga
    34261 ctgttttgcc ggtgtctggg ggcatagtat gcagcagcaa ggcgttctgg aaaccggcga
    34321 tctggaagag gcgctgaacg cggcgcaggc catcggcgat gaccgtttac aacagcaaag
    34381 tcaggggcga gtagtaccag atagtttcac acatggcact tctcagcaac gctacagctg
    34441 gtttaaacgt ggtttcgaca gcggcgatcc ggcacaatgc aatacttttg gtaaaagcat
    34501 ttaacttccg gggcagggat ggctgaactg actgcgcttc acacattaac agcgcaaatg
    34561 aaacgtgaag ggatccgccg cttgctggtg ttgagcgggg aagagggttg gtgttttgat
    34621 catgcgctta agttgcgtga tgccttacct ggcgactggc tgtggatttc gccgcagcca
    34681 gatgctgaaa accactgttc accctcggcg ctacaaactt tacttgggcg cgagttccgg
    34741 catgcggtat tcgacgcccg ccacggcttt gatgccgctg cctttgcagc acttagcgga
    34801 acgttgaaag cgggaagctg gctggttttg ttactccctg tatgggaaga gtgggaaaac
    34861 caacctgatg ccgactcgct gcgctggagt gattgccctg accctattgc gacgccgcat
    34921 tttgtccagc atctcaaacg cgtacttacg gcggataacg acgctatcct ctggcggcaa
    34981 aaccagccgt tctcgttggc gcattttact ccccgtactg actggcaccc cgcgaccggc
    35041 gcaccacaac cagaacaaca gcaactctta cagcagctac tgaccatgcc tccgggcgtg
    35101 gcagcggtaa cggctgcgcg tgggcgcggt aagtcggcgt tggcagggca actcatttct
    35161 cgtattgcgg gcagtgcgat tgtcaccgcg cccgcaaaag cggcaacgga tgtactggca
    35221 caatttgcgg gcgagaagtt tcgctttatt gcaccggatg ccttgttagc cagcgatgag
    35281 caagccgact ggctggtggt cgacgaagcc gcagccatac ctgcgccgtt gttgcatcaa
    35341 ctggtatcgc gttttcctcg aacgttgtta accactacgg tgcagggcta cgaaggcacc
    35401 ggacgtggtt ttttgctgaa attttgcgct cgctttccgc atttacaccg ttttgaactg
    35461 caacagccga tccgctgggc acagggatgc ccgctggaaa aaatggttag tgaggcactg
    35521 gtttttgacg atgaaaactt cacccataca ccacaaggca atatcgtcat ttccgcattt
    35581 gaacaaacgt tatggcgaag cgagccagaa acgccgttaa aggtttatca gttattgtct
    35641 ggtgcgcact accggacttc gccgctggat ttacgccgga tgatggatgc accagggcaa
    35701 cattttttac aggcggctgg cgaaaacgag attgccggag cgctgtggct ggtggatgag
    35761 gggggattat ctcaacaact cagtcaggcg gtatgggcag gttttcgtcg cccgcggggt
    35821 aatctggtgg cccagtcgct ggcggcgcac ggtagcaatc cactggcagc gacattgcgt
    35881 ggacggcggg tcagccggat agcagttcat ccggcgcgtc agcgggaagg cacagggcag
    35941 caacttattg ctggtgcttt gcaatatacg cgtgacctcg actatctttc ggtgagtttt
    36001 ggttacaccg gggagttatg gcgtttctgg caccgctgcg gttttgtgct ggtgcggatg
    36061 ggtaatcatc gtgaagccag cagcggttgc tatacggcga tggcgctgtt accgatgagt
    36121 aatgcgggta aacaactggc tgaacgtgag cattaccgtt tacgtcgcga tgcgcaagct
    36181 ctcgcaaagt ggaatggcga aacgcttcct gttgatccac taaacgatgc cgtcctttct
    36241 gacgacgact ggcttgaact ggccggtttt gctttcgctc atcgtccgct attaacatcg
    36301 ttaggttgct tattgcgtct gctacaaacc agtgaactgg cattaccggc gctgcgtggg
    36361 cgtttacaga aaaacgtcag cgacgcgcag ttatgtacca cacttaaact ttcaggccgc
    36421 aagatgttac tggtccgtca gcgggaagag gccgcgcagg cgctgttcgc acttaatgat
    36481 gttcgcactg agcgtctgcg cgatcgcata acgcaatggc aattttttca ctgactcctt
    36541 caattttctc ccatggtcat tgtgagtcag cggcgtaaaa ttaatgacat cagttaagga
    36601 gttcgccatg aaacatgacc attttgttgt tcaaagcccg gataaacctg cgcaacagtt
    36661 gttactgctt ttccacggtg tcggggataa cccggtggca atgggggaaa taggtagctg
    36721 gtttgcaccg ctgtttcctg atgcgctggt ggtgagtgtc ggtggcgcgg agccgagcgg
    36781 taatccggcg gggcgtcagt ggttttcagt gcagggtatt acggaagata atcgccaggc
    36841 gcgtgtggat gccatcatgc cgacgtttat tgaaacggta cgctactggc agaaacagag
    36901 cggagtaggg gctaacgcca cggcgctaat tggtttctca caaggcgcga ttatggcgct
    36961 ggagagcatt aaagccgaac cgggccttgc ttcgcgcgtc attgccttta acggacgcta
    37021 tgcgagcctg ccggagaccg cgtctacagc cacgacaatt cacttgattc atggtggtga
    37081 agatccggtt atcgatctgg cgcatgcagt tgcggcgcag gaagcgttaa tcagtgccgg
    37141 tggtgacgtg acgctggata tcgtggagga tttaggccat gcgatcgaca atcgcagtat
    37201 gcaatttgcc ctggatcact tgcgttatac cattccgaaa cattatttcg acgaagcgct
    37261 aagtggcggt aagcctggcg atgatgatgt gattgaaatg atgtaaaaga cagattactt
    37321 ggcctgataa gacgcgtgag cgtcgcatca ggcatcgttc accattgccg gatgcggcgt
    37381 taacgcctta tccggcctac ggttgagcac tcagcttact tcttcggttg atcctttttc
    37441 ggccagtcgt cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg aagctccggc
    37501 ttgttggcga gaaatttctt atggtcgacg cgcttgagat ctttaatcac gttcagcagg
    37561 acgcctacca aaaagacaat caccagaatc caccaatatt tagccagcca gtccatgctt
    37621 atttcctctt gcagaaccac tcatcaggcg acgagctgtt ccatgatacg ttgatacata
    37681 cgggcaagta gctgcaggtt ggcagcgttc acacattcat taattttatg aatagtggca
    37741 ttgaccggcc cgagttccac cacctgcgcc cccatgcgag caataaagcg cccgtcggac
    37801 gttccgcctg tggtcagtag ctgcggttta atttcattat agtgctcaac cgcgttaacg
    37861 accgcatcca ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag ccaccagtcc
    37921 accgtataac gcagttgatg tttttcaagc agggcaagca cctgtgcttt gatcatctca
    37981 tcagtcagtt cggtgctgaa gcggaagtta aactgcacaa acagttcacc cggaataacg
    38041 ttgttactgc ccgttcccgc ctgaatattg gcaatctgca tactggtcgc cgggaagaat
    38101 tcattgccct gatcccactc aatagccact aattcattaa ggaaaggtgc tgcgcgatgt
    38161 accggattgt cagccagatg cgggtaggca acatgcccct gaacgccatg aatggtgagg
    38221 ttgcaggtta acgatccgcg acgaccattt ttcaccacat cacctaccac ttcgatactc
    38281 gacggttcgc cgaccaggca gtaatcgaga cgctcattac gtgccattaa cgcttcgacg
    38341 acttttaccg taccgttgtg tgcactggct tcttcatcag aggtgatcag aaatgccagt
    38401 cgccccgtat ggttgggatg ttgtgcgaca aagcgttctg ccgctaccac catcgccgcc
    38461 agcgagcctt tcatatctgc cgcaccgcgc ccgaataaca tgccatcacg aatggtgggt
    38521 tcaaacggcg gattgatcca acgatcggcg tcgccaggcg gcaccacgtc ggtatgcccg
    38581 gcaaaggcta acgtttcacc ttgcccacgc catgcccaaa aattctgcgt atcggcaaag
    38641 tccatgcgtt caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa agcctggcat
    38701 cctgcgtcat caggactcag ggaagggcgg cgaataagct gttgtgtcag ctcaataacc
    38761 gggcacgaca tagactacac ctcatggaaa aattgctgat aactggaatc actgaaaccc
    38821 agcagcatag gcttaccagg cgcgcagagc aatggacgtt tgataattgc aggcatttca
    38881 gtcattaatg ccgccgcaga ggccgcatcg gtgattttat tgcgggtggt ttcgtccagt
    38941 ttacgccagg ttgtaccacg ggtgttgagt aacgcttccc agcctaattc gttgataaaa
    39001 ccgttcaata attcgctgtc cagcccatcg acgcggtaat catgaaaacg atagtcgatg
    39061 ttattggctt ccagccaacg gcgagccttt ttaatggtgt cacaattttt gatgccgtaa
    39121 agtgtaacca taatgaatcc tttttcgatt tatgagaata aatactcatt taagggtgaa
    39181 aacattttgc ctgcaaatga ttatca
//
LOCUS       DAZFKM010000030        38838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFKM010000030 DAZFKM010000000
VERSION     DAZFKM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            29..1078
                     /gene="yeiH"
                     /locus_tag="ACHKAY_001702"
     CDS             29..1078
                     /gene="yeiH"
                     /locus_tag="ACHKAY_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO5700395.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1152..2009
                     /gene="nfo"
                     /locus_tag="ACHKAY_001703"
     CDS             1152..2009
                     /gene="nfo"
                     /locus_tag="ACHKAY_001703"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO5700396.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2013..3101
                     /locus_tag="ACHKAY_001704"
     CDS             2013..3101
                     /locus_tag="ACHKAY_001704"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5700397.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3157..4407)
                     /gene="nupX"
                     /locus_tag="ACHKAY_001705"
     CDS             complement(3157..4407)
                     /gene="nupX"
                     /locus_tag="ACHKAY_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5700398.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4507..5448)
                     /gene="rihB"
                     /locus_tag="ACHKAY_001706"
     CDS             complement(4507..5448)
                     /gene="rihB"
                     /locus_tag="ACHKAY_001706"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO5700399.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5578..6276
                     /gene="yeiL"
                     /locus_tag="ACHKAY_001707"
     CDS             5578..6276
                     /gene="yeiL"
                     /locus_tag="ACHKAY_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO5700400.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6344..7594)
                     /gene="psuT"
                     /locus_tag="ACHKAY_001708"
     CDS             complement(6344..7594)
                     /gene="psuT"
                     /locus_tag="ACHKAY_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO5700401.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7688..8626)
                     /gene="psuG"
                     /locus_tag="ACHKAY_001709"
     CDS             complement(7688..8626)
                     /gene="psuG"
                     /locus_tag="ACHKAY_001709"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO5700402.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(8614..9555)
                     /gene="psuK"
                     /locus_tag="ACHKAY_001710"
     CDS             complement(8614..9555)
                     /gene="psuK"
                     /locus_tag="ACHKAY_001710"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO5700403.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            9740..9886
                     /locus_tag="ACHKAY_001711"
     CDS             9740..9886
                     /locus_tag="ACHKAY_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700404.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            complement(9979..11670)
                     /gene="fruA"
                     /locus_tag="ACHKAY_001712"
     CDS             complement(9979..11670)
                     /gene="fruA"
                     /locus_tag="ACHKAY_001712"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO5700405.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(11687..12625)
                     /gene="fruK"
                     /locus_tag="ACHKAY_001713"
     CDS             complement(11687..12625)
                     /gene="fruK"
                     /locus_tag="ACHKAY_001713"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO5700406.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12625..13755)
                     /gene="fruB"
                     /locus_tag="ACHKAY_001714"
     CDS             complement(12625..13755)
                     /gene="fruB"
                     /locus_tag="ACHKAY_001714"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO5700407.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14123..15304
                     /gene="setB"
                     /locus_tag="ACHKAY_001715"
     CDS             14123..15304
                     /gene="setB"
                     /locus_tag="ACHKAY_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO5700408.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15301..15555)
                     /gene="yeiW"
                     /locus_tag="ACHKAY_001716"
     CDS             complement(15301..15555)
                     /gene="yeiW"
                     /locus_tag="ACHKAY_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5700409.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15710..16282
                     /gene="yeiP"
                     /locus_tag="ACHKAY_001717"
     CDS             15710..16282
                     /gene="yeiP"
                     /locus_tag="ACHKAY_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO5700410.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16505..17971
                     /gene="yeiQ"
                     /locus_tag="ACHKAY_001718"
     CDS             16505..17971
                     /gene="yeiQ"
                     /locus_tag="ACHKAY_001718"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5700411.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18089..19075
                     /gene="yeiR"
                     /locus_tag="ACHKAY_001719"
     CDS             18089..19075
                     /gene="yeiR"
                     /locus_tag="ACHKAY_001719"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO5700412.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19114..19827
                     /gene="lpxT"
                     /locus_tag="ACHKAY_001720"
     CDS             19114..19827
                     /gene="lpxT"
                     /locus_tag="ACHKAY_001720"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO5700413.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20239..20805
                     /gene="mepS"
                     /locus_tag="ACHKAY_001721"
     CDS             20239..20805
                     /gene="mepS"
                     /locus_tag="ACHKAY_001721"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO5700414.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20986..22542
                     /gene="pdeN"
                     /locus_tag="ACHKAY_001722"
     CDS             20986..22542
                     /gene="pdeN"
                     /locus_tag="ACHKAY_001722"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO5700415.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSYLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22624..24438
                     /gene="yejA"
                     /locus_tag="ACHKAY_001723"
     CDS             22624..24438
                     /gene="yejA"
                     /locus_tag="ACHKAY_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO5700416.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            24439..25533
                     /gene="yejB"
                     /locus_tag="ACHKAY_001724"
     CDS             24439..25533
                     /gene="yejB"
                     /locus_tag="ACHKAY_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO5700417.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25533..26558
                     /gene="yejE"
                     /locus_tag="ACHKAY_001725"
     CDS             25533..26558
                     /gene="yejE"
                     /locus_tag="ACHKAY_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5700418.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26560..28149
                     /gene="yejF"
                     /locus_tag="ACHKAY_001726"
     CDS             26560..28149
                     /gene="yejF"
                     /locus_tag="ACHKAY_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO5700419.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28153..28497)
                     /gene="yejG"
                     /locus_tag="ACHKAY_001727"
     CDS             complement(28153..28497)
                     /gene="yejG"
                     /locus_tag="ACHKAY_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO5700420.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28830..30020)
                     /gene="bcr"
                     /locus_tag="ACHKAY_001728"
     CDS             complement(28830..30020)
                     /gene="bcr"
                     /locus_tag="ACHKAY_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO5700421.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(30048..30743)
                     /gene="rsuA"
                     /locus_tag="ACHKAY_001729"
     CDS             complement(30048..30743)
                     /gene="rsuA"
                     /locus_tag="ACHKAY_001729"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO5700422.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30892..32652
                     /gene="radD"
                     /locus_tag="ACHKAY_001730"
     CDS             30892..32652
                     /gene="radD"
                     /locus_tag="ACHKAY_001730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5700423.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32777..33061
                     /gene="rplY"
                     /locus_tag="ACHKAY_001731"
     CDS             32777..33061
                     /gene="rplY"
                     /locus_tag="ACHKAY_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO5700424.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33200..34207)
                     /gene="yejK"
                     /locus_tag="ACHKAY_001732"
     CDS             complement(33200..34207)
                     /gene="yejK"
                     /locus_tag="ACHKAY_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO5700425.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34389..34616
                     /gene="yejL"
                     /locus_tag="ACHKAY_001733"
     CDS             34389..34616
                     /gene="yejL"
                     /locus_tag="ACHKAY_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO5700426.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34636..36396
                     /gene="yejM"
                     /locus_tag="ACHKAY_001734"
     CDS             34636..36396
                     /gene="yejM"
                     /locus_tag="ACHKAY_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO5700427.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKQDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36471..36547
                     /locus_tag="ACHKAY_001735"
     tRNA            36471..36547
                     /locus_tag="ACHKAY_001735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36505..36507,aa:Pro,seq:ggg)
     gene            36766..38007
                     /locus_tag="ACHKAY_001736"
     CDS             36766..38007
                     /locus_tag="ACHKAY_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5700428.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            38106..38336
                     /locus_tag="ACHKAY_001737"
     CDS             38106..38336
                     /locus_tag="ACHKAY_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700429.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            38504..38709
                     /locus_tag="ACHKAY_001738"
                     /pseudo
     CDS             38504..38709
                     /locus_tag="ACHKAY_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            38715..>38838
                     /locus_tag="ACHKAY_001739"
     CDS             38715..>38838
                     /locus_tag="ACHKAY_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700430.1"
                     /translation="MTDMTNTKKAAPIWSRLSEQLTRCALCVCDPKHKHGDDSRY"
BASE COUNT         9629 a        10072 c         9575 g         9562 t
ORIGIN      
        1 accttatgcc cggttaaagg agagggttat gacgaatatc accttacaga aacaacatcg
       61 tacactgtgg cattttattc cggggttagc cctgagtgca gttatcaccg gggtcgccct
      121 gtggggtggt tccattcccg cggttgcggg tgccgggttt agtgccctca ccctcgcaat
      181 cttgttgggg atggttttag gcaacaccat ctatccgcac atctggaaaa gctgtgacgg
      241 tggtgtgctg tttgccaagc aatatttatt acgtctgggt atcattcttt atggcttccg
      301 tctgacgttc tcgcaaattg ccgatgtcgg tatcagtggg atcatcattg acgtgttgac
      361 gctgtccagt accttcctgc tggcttgctt cctggggcag aaagtgtttg gtctggataa
      421 gcacaccagc tggttgatcg gtgccggtag cagtatctgt ggtgctgccg cggtactggc
      481 gactgagccg gtagtgaaag cggaagccag taaagtaacc gtggctgttg caaccgttgt
      541 tatcttcggg accgtcgcta ttttcctcta cccggcgata tatccgctga tgtctcaatg
      601 gtttagtccg gaaactttcg gtatctacat tggttctact gtgcacgaag tggcgcaggt
      661 ggtggcggca ggtcatgcca tcagcccgga tgcggaaaac gcagcagtta tttccaaaat
      721 gctgcgcgtg atgatgctgg ctccgttcct catcctgctg gcggcgcgtg ttaaacagct
      781 gtctggggcg aacagcggcg agaaaagcaa aatcactatt ccgtggtttg ccatcttgtt
      841 catcgtagtt gccatcttta actcgttcca cctgttaccg cagagcgtgg tgaacatgct
      901 ggtaacgctg gataccttcc tgctggcaat ggcgatggcg gcgctgggtc tgaccactca
      961 cgtcagcgcg ctgaaaaaag ccggggcgaa acctctgctg atggcactgg tactgtttgc
     1021 ctggctgatt gttggtggtg gtgcgattaa ctatgtgatt caaagcgtca tcgcataaac
     1081 cactacatct tgctcctgtt aacccgctat cattaccgtt ttcctccagc gggtttaaca
     1141 ggagtcctcg catgaaatac attggagcgc acgttagtgc tgctggcggt ctggcaaatg
     1201 ccgcaattcg cgccgccgaa atcgacgcaa ccgcgtttgc cttgttcacc aaaaaccaac
     1261 gtcagtggcg tgccgcaccg ctcacgacgc aaaccatcga tgaattcaaa gcggcctgtg
     1321 aaaaatatca ctacacatcg gcgcaaattc ttccccacga cagctatctg attaacctcg
     1381 gacatccggt cactgaagct ctggaaaaat cgcgcgatgc ctttatagat gaaatgcagc
     1441 gttgcgaaca actggggctt tctttgctca acttccaccc tggcagccat ctgatgcaga
     1501 tttcagaaga ggattgcctt gcgcgtattg ccgaatccat caacattgcg ctggataaaa
     1561 ctcaaggtgt aacagcggtg attgaaaaca ccgccggtca gggcagtaac ttagggttta
     1621 aattcgaaca tctcgcggcg attatcgacg gcgtggaaga taaatcccgt gtcggcgtct
     1681 gcattgatac ctgccatgct ttcgctgccg ggtatgattt gcgtactcca gccgaatgcg
     1741 aaaaaacgtt cgctgatttt gcccgtattg ttggctttaa gtatctgcgc gggatgcacc
     1801 ttaacgatgc gaaaagcacc tttggcagcc gcgttgaccg ccatcatagc ctcggtgaag
     1861 gcaatatcgg tcatgatgcg ttccgctgga tcatgcagga cgaccgtttc gacggcattc
     1921 cgctgatcct tgaaaccatc aacccggata tctgggcaga agagatcgcc tggctgaaag
     1981 cgcaacaaac tgaaaaagcg gtggcctgat cgatgaacga cagggaaaag cagattctta
     2041 aaattttacg gcgtaacccg ctgattcaac agaatgaaat cgccgatatt ctgcaaatca
     2101 gccgctcacg tgttgccgct catattatgg atttgatgcg caagggtttg attaaaggca
     2161 aaggatatat ccttaccgag caggattact gtgtggttgt gggtgcaatc aatatggata
     2221 ttcgcggaat ggcggatatc cgttacccgc aagcagcctc tcatccaggt tctgttcact
     2281 gttccgcagg cggcgtcgga cgtaatatcg cgcataacct ggctctgctt ggtcgggatg
     2341 tgcatttaat atcagctatt ggcaacgatt tttacggcga gacattactg gaagaaacgc
     2401 gccgcgcggg cgtgaacgtt tcaaactgca ttcgtctgca tggacacagc actgcaactt
     2461 atctcgctat cgctaataag caggaagaaa ctatcctggc aatcaatgac acacatattc
     2521 tgcaacagct aacgcctcaa ctgctcaata cttcacggga cttgattcgc catgctggtg
     2581 tggtgctggc tgattgtaat ctcacacctg aagccctgga gtgggtcttt accattgctg
     2641 atgaaattcc gatgtttgtc gataccgttt ccgaatttaa agcgaacaaa gtaaaaaact
     2701 ggtacagccg gatccacact ctgaaaccaa cacaaaatga actggaaatt ttgtgggggc
     2761 aaccgattaa agacgacaac gatcgcatca gagctgtaaa ttcgctgcat caacaaggtg
     2821 ttaaacgcat ttttgtttat ctgaaagatg aatctgtatt ttgcagcgat aaggatggag
     2881 aacaattttt gctgaccgcg ccagcgcata cgacagtaga cagttttggt gctgacgatg
     2941 gttttatggc gggcctggta tatagctttc ttgaaggata cagtttccgc gacagcgccc
     3001 gttttgcggt agcctgcgcg gcaatttcgc gcgccagcgg cagcttaaac aaccctaccc
     3061 tgtctgccga taacgcgctt tcattagtgc caatggtgta acaatgttgc cggattcgac
     3121 gcttgaccgc atcttatccg gctcataaat tgcgcactac gctaaaccaa taaagaatcc
     3181 tgcaatagta gcactcatca ggttagaaag tgtcgccgcc gccagcgcgc gtaaaccaag
     3241 ttgggcgatt tccggcgcac ggtgtggcgc aaccgcagaa aacgccccca ccaccacccc
     3301 gatagaacca aagttagcga aaccgcacaa cgcgaaagaa ataatcgcca cggttttagc
     3361 atccagagtg ccacccgttt gcagatatgg cgagaaattg agataagcga caaattcatt
     3421 gatcgccagc ttctgcccaa tcaaactccc ggcaagattt gcatcactcc agtcaacccc
     3481 catcacccac gccaatgggg ccaacaggta acctaaaatg gactccagcg aggcatgtgc
     3541 aaaaccaaac cagccgccaa cgccgccgat aataccatta attaacgcaa tgatggcgac
     3601 aaatgccata acaactgtcg ctacaccggc ggcgattttc agcccggtca ttgcccctgt
     3661 cgcagcggct tcaataatgc tttttggcgg tgtttcggtg aaagagaggt tattaaagga
     3721 aacctgcgaa gattccgtag ccgggcttaa caggcgggca aacaagatcc cgcctgggat
     3781 cgccattaac gatgccgcca gcaaatattc cacaggtacg cccagggcgg cgtaaccaat
     3841 cattgtcgaa ccagcaatcg aggccatgcc actacaaatc gctgtaaata attcattgcg
     3901 attcagacga tcgataaagg gtttgacgat tgccggaatt tcgttttgcc cgaggaaaat
     3961 ggtagtgacc gcgacgaatg actcgatctt gctgatattt aacgctttct ggaagatacc
     4021 gccgagaatg cgaattaaaa tccccatcac accgatgtag tagagaatac tcaccagtgc
     4081 tgtaacgaag ataattgccg gtaatactct gaaaccaaag ataaatcctg cgccatcaaa
     4141 cagcgtgtcc attttcggcc cgaccagcga accgaagata aatgcgctac ccgcgtcgct
     4201 gtacgccatc actttatgca cgccaaaagc gactttttca gcgacccaac gccctggcgg
     4261 taaccaaagc ataatgccgc caatcacaac ctgtaacact aacgccgcgc caacggtacg
     4321 caggctgatc ttcttcttgt ttactgacag caaaaatgca attgccagca ataccaccat
     4381 tcccagaaca cttctcatga catccatagt gattatctct tcatgtcata ttgggcgacg
     4441 gctttctgcc catcagaaat agccgggcct gcatcttgcg ctatcggccc ggcttgttat
     4501 caacgcttaa tgggttttga tgtagccgcg cacgcactct tcgactaagc cccagaacca
     4561 gtcggtatca atagtgatgc cgactttggt attggcgggc ttgccaagaa cgcccagctc
     4621 gtcgcagacg gtgcgcccat agcaagggcc actgttgacg tccacttcga cgtacatctc
     4681 ctgggtttta atgccatcag ggttaatcag ataaccgatg caggtggcgt cgtgcaccgg
     4741 gccgccagcc aggccgtagt tttcgaactg cgttttgaga gtgaagttca tgatgtcgct
     4801 gaacagctct ccggcagggc cgcctgccct ttccatccga gcaatcacgt ccggggtgca
     4861 aacggtctgg ttggtgagat cgaggcccat catcactaat ggaacgccag aggtgaacac
     4921 cacgcgggct gcttccgggt cagcaaagat gttgaactcg gcagatggcg tgaagttgcc
     4981 tgtaccgtaa gcgccgccca ttagcacaat ttcacggatt ttgggcagga tcgcgggttg
     5041 catacgcatt gccaccgcga tatttgaaag cggaccaacc ggcaccagag tgatatcgcc
     5101 atcgcttgcc atcagggtat caatgatgta tttcaccgcg tgggtacttt ctgcctggcg
     5161 ggttagcggc tcgaataccg ggccatccag tccggtttca ccgtgaatat tatcggcaac
     5221 gatttgttga cgcataatgg gttgcggcat ccccgcgtaa accggaacat taatctccag
     5281 tttctggcaa acattcaggc cattaattaa tgttttatca agcgtctgat tacccgctac
     5341 aatagtgatg cctaataaat ctattgccgg atgtttcgct gccatcatca ttgcaatagc
     5401 atcatcatga cccggatcac aatccagaat aatttttctc ttttccattg tttatttcct
     5461 ctgtttccag ttgcgttatt ttttctacag caaagaaaat tcgaccgggc cgatgattga
     5521 atcttaccaa ccgcgtacgt atgctaaata tgagaaatct catagcggat aaacatcgtg
     5581 aaagaaatcc acaataatga tcttaagcag caattgatga gtgaatccgc gtttaaggat
     5641 tgctttttaa cggatgtttc agccgatacg cggctgtttc attttttagc gcgtgactac
     5701 attgtgcagg aagggcaaca gccgtcctgg ctgttttacc tgacgcgagg ccgcgccagg
     5761 ctttacgcca cgctagctaa tggtcgcgtg tcgctgatcg atttctttgc cgccccctgt
     5821 tttattggcg agattgagtt aatcgacaaa gaccatgaac cgcgtgcggt gcaggctatt
     5881 gaagagtgtt ggtgccttgc gctccctatg aaacattacc gcccgcggtt attaaacgac
     5941 acgctatttt tacgaaaact ctgcgtcacc ttaagtcata aaaattatcg taatattgtt
     6001 tctttaactc agaatcaatc atttccgtta gtgaatcgcc tggcagcatt tatattactc
     6061 tcgcaggaag gtgatcttta tcacgaaaag catacgcaag cggcagagta tttaggcgtt
     6121 tcttatcgac atcttttata tgttctcgcg caattcattc acgacggttt attaattaaa
     6181 agcaagaaag ggtatctaat taaaaacaga aagcagttgt caggactggc gctggagatg
     6241 gacccggaga ataaattctc cgggatgatg cagtaaaaat tatttgcaat agcgcgattg
     6301 ccggatgcaa cacttaataa gcgcgtacat ccggcaatga aactcaagct aaaccaataa
     6361 agaacccggc aatggtcgca ctcatcaggt tggaaagcgt tgctgctgcc agcgcccgta
     6421 aaccaagctg ggcgatttcc ggcgcgcgtt ttggcgaaat agccgaaaat gcgccaacga
     6481 caacaccgat agaaccaaag ttagcaaaac cacaaagcgc aaaggagata atcgcaatgg
     6541 ttttcacttc cagcgtgccg cccgtttgca ggtatgggga gaaactcagg taagcgacga
     6601 attcgttaat cgccagtttc tgcccaatca ggctacccgc aagattggca tcactccagt
     6661 ccacacccat gatccacgcc agcggtgcca gcacatagcc aaaaatactt tccagagagg
     6721 cattggcgaa accaaaccag ccaccaattc cgccgataat gccgttgatc agcgcaataa
     6781 ttgcgacaaa cgccattacc accgtcgcta caccagcggc gattttcagc ccggtcatcg
     6841 caccgctcgc cgccgcttcg ataaagcttt ttggcggcgt ttcgctgaac gacagatttt
     6901 caaatgtgac ctgtgaaggc tcggtggccg ggctaagaat acgtgcaaac aaaataccac
     6961 ctgggatcgc catcagcgat gccgctaaca ggtagtcaat tggtacgccc attccggcat
     7021 aaccaatcat catcgaacca gcaatggacg ccatcccgct acaaattgcg gtaaacaact
     7081 cgttgcgatt catgcgatcg ataaacggtt taacgatcgc cgggatctca ttttgcccga
     7141 ggaaaatagt tgtgactgcg acaaaagatt cgattttgct gatgttaaga gctttctgga
     7201 aaatgctgcc aaggatgcga atcagcagcc ccatcacgcc aatgtagtac agcagactga
     7261 tgagcgcagt aacgaaaata atcgccggaa gtacgcgaaa ggcgaagata aaacccgcac
     7321 cgtcaaacag gacatccatt ttcggcccaa ccagcgaacc aaaaatgaag gcgctaccgg
     7381 catcactgta agacatcact ttatgaacgc ctaatgccgc ctgttctact gcccactttc
     7441 ccggtgggaa gtagagcatg atgccaccaa tagcgatttg cagcagcagt gcggctccaa
     7501 ccgtgcgcaa actgatgctc tttttattca ctgacaacag aaatgctatt gccagtaaca
     7561 ccaccatccc cacaacactt ctcattatat ccataatgat tttcccttca tgccagtaaa
     7621 cccggcgtca gcgccaggtt ttggtatgct tgatgagtac gggcgacggc tttctgcccg
     7681 tcagaaatta acccgcgaga cgctggtatt ctttggcaat ttcactcgcc agaatggcgt
     7741 tgttgaacac cagctggata ttggatttca ggctgtcacc gccggtcagc tcagcaacgc
     7801 gcgccagcag gaacggtgta ctttctttac caataacgcc ctgctcttcg gcttcggcga
     7861 cggcttgatc tatggctgca ttgatagatt cctccggcat cgcaaactgt tccgggatcg
     7921 gattcgccac taccaggccg ccgttcaaac cggtttgcca tttcactgcc attgcacgag
     7981 cgatttccgt ggcactatca aggcgaatac tgacttcaaa tgggctggta cggcagaaaa
     8041 acgcaggcag cgcttttgtc tgatagccaa ttaacggcac accgaaggtt tctaaatatt
     8101 ctgttgttaa tcctaaatcg agaatagatt tcgccccggc acaaacaacg gtgacattag
     8161 tatttgccag ttcttgcaaa tcggcagaaa tatcgaaggt atgttccgcc ccgcgatgca
     8221 caccaccaat tcccccggtg gcaaatactt taattccggc aagcgccgca ataatcatcg
     8281 ttgaagccac agtggttgcg ccattttttc cggcggcaac aacaaaaggt aaatcgcgac
     8341 gactaacttt ggtcacgtta tgcccttcac gacccagtaa ttcaatttct tctttgctta
     8401 aacccacttt catcacgccg ccaataatgg cgatagtggc aggtactgcg ccctgtttac
     8461 gaatagtttc ttctacttca attgcggtct gggcattttg tgggaacggc atcccgtgag
     8521 aaataatggt cgattccagc gccacaaccg gttttttgtt ttttaaagcg tcctgcactt
     8581 ccggggaaat ttgtaataat tcaggggaaa tttttaattc agacattctg cgttctccac
     8641 taacgatata acgttggcaa tcgataaatc ggggttattg gtgtattcac aggagagcgc
     8701 cattgacgaa catccctgtg cgaaacgaac agattcggca aacggcattc cgtctaccca
     8761 acacgaagca agtcccgcca tcatggcatc gcccgctccg gtaacattaa taacattggt
     8821 tttgatcggg gcagaccagc cactttcacc gctgatatca ctgtaataaa cgccatcgcc
     8881 gcccatgctc aataccagtc ggttcaggcc atgttgatgg aaccaggcag caacttttgc
     8941 cacatctcca cgccctgaca gcgcaatccc actcagtgtt tccgcttcaa ggcggtttgg
     9001 cttcagagtg tggatctgat ttagacggtc gcgcactttg acacatttcc acgctgaaac
     9061 cggatcgaca aatacgggta cgttggcagc attatccaga atccatgcca gtgcctcttc
     9121 actgatatta cagtccgcga caatgacctt tgccctctga ataaattcac ggtgctgtgc
     9181 gagatattca gctgtaatag cgttgctaat attcatgtca tttatagcaa ccagcatttc
     9241 accggtatta tcgagtaatg ataaataact cgacgtattt tctcccggca caatcaggca
     9301 tttatcgaca taaacgccag attgattggt ttgcgttagc agcgattgac cataaaaatc
     9361 actacctact gcgctcagta accaggcttt gttacccagc aacgccaggt tttgtgcaat
     9421 atttcgccct actccaccag gtgtaaattt tattttacct ggatttgaat ccgcataatt
     9481 taatgattca tgtgaatatc cggcgacatc aatattcgcc gaacctataa ttacgacata
     9541 atccttttcg cgcatagcac gtcctctggt aaaaaattac caaccctgtc aatccacaag
     9601 aatatagata ctgcaaagac agtttaaaca tgtgttcata tttaaacatg tgctcatagt
     9661 atgtatgttt tacgtaaagt aaattgccgc cagagaggca aagcgggcag atttgagcag
     9721 gctcacaaaa tgcgattaaa tgccagaatt acaaaagcga ggaatttgtg acgggatgca
     9781 cagaacagga acactaaaaa agggcagaaa agaatctgcc cttgaggaaa taaccttagt
     9841 gtttgatgaa cgtttcaggt ttctactgaa agataaaacc atttaaattc ataaaattac
     9901 attcacaaac cggtcattct ctttgcgctc atccaatgat acagggcagt aaatttctgc
     9961 cctgtaacac accttttatt acgcggcttt cgctactgcg tccacttccg gacgtttcag
    10021 gaaggcatag gccaaacccg ccaccagcgt accggcaata attgctacca ggtaacccag
    10081 taccggcgta atagcgccag ggatcagcag aacaaacaga ccaccgtgcg gtgccatcag
    10141 tttcgcacca atcgccattg agattgcgcc agtcagcgcc ccaccaacga tacaacacgg
    10201 cagcacacgc atcggatcac gagcagcaaa cggaattgca ccttccgaaa tgaagcacag
    10261 tcccaatacc agagcggctt tgccaccttc ctgctgcgct ttgtcgaatt tgcgacgcgc
    10321 caccattgtt gccagaccca ttgccagcgg tggcaccata cctgccgcca taatcgccgc
    10381 catcgggcca taggtttgag tactcagcag acccacaccg aatgcgtacg ctgctttgtt
    10441 taccggaccg cccatgtcag tacacatcat gccaccgagg atcgccccca gcagaaccgc
    10501 attcgcagtc cccatggtct gcagccagtg agtcaaccct tcgagaatgc cagcaactgg
    10561 tttaccgatc aggtagatca tcgccagacc gaccaccaga ctggaaatta gcggaatgat
    10621 caggatcggt ttcagcgcct ccatactctg tggcagtttc agttgcgtac tgattaactt
    10681 cgcaatgtaa ccagccagga agcccgcaat aataccgcca atgaagccag aaccggtgct
    10741 gaccgccagc ataccgccaa tcagacccgg cgtgagaccc ggacgatcgg caatggagaa
    10801 ggcaatgtaa cctgccagta ccggcaccat cagcgcaaag gctgaaccac caccaatctg
    10861 catcagcgcc gctgccaacg tacccggctc tttaaacgct tcgataccaa aagcaaaaga
    10921 aagcgcgata cacagaccac ctgcaacgac catcggcagc atgtaagaga cgcccgtcag
    10981 caagtgacgg tatgcgcctg cactctcttt cttaccttca gaggtcgccg tttgagcttt
    11041 gcccgccggt tcatacggcg ttgcttcagc aaccgcttta tccagttcct gcgcggtttt
    11101 cttcagcgcc agaccggtag aggtacgata catcggttta ccagcaaatt tcgccagatc
    11161 cacttcgata tctgccgcca caatcaccag atccgctgcg gctacttctt cgggggtgat
    11221 tgcattaccc gcaccaacag aaccacgggt ttcaactttc acccaccagc cacgtttttt
    11281 cgcttcggtt tcaatggctt cagccgccat aaaggtgtgt gctacgccag tcgggcaagc
    11341 agtcaccgca actacgcgtt tcggaccgct ggcggcaacc ggtgctgtcg cagcgaccgg
    11401 cgcagtgtaa ggtttcgcat ggcctttggc ttcactcagg aacagctcag ggtgcgcaac
    11461 tgcccgggaa atatcaccca gccagacatt tttaccgttc agcgcgctgt cattcgggat
    11521 ggaatcaccg agaacaatcg ccatttctgc gtcgttcgga ttgtcgatga tttccagttt
    11581 tgcttttcgc gccgccgcgc ccagcagggt cttcgccata taggcgcgtg cctgaccgag
    11641 attagcgtca ataatcagca gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt
    11701 gtaagtcgac gcgcgccatc attgcggcca actgcggacg atcggtaata cccacattgc
    11761 tttgacttac cgccagggct gcaacagcag tcgccagacg cagtgtgtgt tcactggatt
    11821 cacgcatcag caagccataa atcaggccac caaccataga atcccctgcg ccaacggtgc
    11881 ttacgacatc gactgacggt ggtttggcga tccattcgcc ggaggcatta acccaaagcg
    11941 cgccttcggc acccagtgaa ataacaacat gcgcaatacc ttgttcacgt agcgcatgtg
    12001 cagcttcaat cacatctttc atttcaggca gtttacggcc tgcccagatt tccaactcgc
    12061 ggcggttagg tttcaccagc cacggtgccg ctttcaaacc tgctactaac gcttcacggc
    12121 tactatcaaa gataatgcaa ggacactgac tacgcaggcg agtcatccag tcggtgaacg
    12181 cttccgggct gacgcctgac ggtaagcttc cgctgacaca gaccatatcg aactgaccga
    12241 gccagctcag agaatcagtc acaaagcgtt cccagtcggc gggggtgact tcaaaacccg
    12301 agaagttgaa gtcggtcact tcgccgtctt tttccgtcag cttaacgtta attcgggtgc
    12361 gcccctgtac aacctggaaa cggttggcaa tgcccagctc gctgaacagt tgctgaaaac
    12421 catcctgatt gtctttaccc aggaagccgc caacggtgac atcaattccc aggtctttta
    12481 atactttggc cacgttgatg cctttacccg ccgcatgcag accggtggtt ttcaccaggt
    12541 tcacttcgcc gcgttcaatt tccgggcaga aaccaacaag gtcataagcc ggattaaggg
    12601 tgatagtagc aacacgtctg ctcattatgc gccctcccca agaccagcag cgatagcgtc
    12661 gccgattgct ttcagcgcct gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg
    12721 acctttctta acgccaagtg ccacaacttt catcagacta cgtccgtttg ccggtttgcc
    12781 ggtgccatca aggtttgtca cggtaatatc actgttaaat tgtttaatgg tgttgaccag
    12841 catggtacct ggacgagcat gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa
    12901 cacgtcgtcg gtcggcgcat catcgctggt cagcagcgcc agcaacgttg ccgcatccgc
    12961 tttcagcaag cggtcagctt tattgtcgag caacaaatca gcgagacgct taagaaccgc
    13021 aatgggctga tcgtcattca tcgccacact caccagcatg gctgccgttt cgccgtccac
    13081 atcaaaagca tttgccgcac ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct
    13141 atcgctcagc cagataccct gtccgaggtt cagcggttgt tcattgatgg ctttggtgac
    13201 gaaagtggcg tcaactgccc tcgcctcttt cagacgcgca gcgttcagcg cctgaagagt
    13261 cagcagatcg ctggcggcga tatccagcgt cagcatttcg ttgtcgagct tcagctgctc
    13321 actctgcttt tcgcccatca gcaatgcgcg aagttcttct gctgttgttg ctgacttcag
    13381 ttgttcagca acggaatcat cgctcagtac gtgggtcagc tggcgtagca ggcccagatg
    13441 ttcatccgag ctggcagcaa taccgatcgc tacgtacgct acctgaccgt caccccaggt
    13501 gacgccttcc gtgaactgaa atacctgaac gccggttttc agcacctgat cgcgggtgtc
    13561 ggtagtgccg tgtggaatag caataccatt gccgaggaac gttgaggttt gctgttcgcg
    13621 cgccagcatg ccattgacgt agccttctgc tacgttaccg gcctgcacca gcgccgcagc
    13681 gagctggcga atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac
    13741 ggataactgg aacatagttc tcctctcttg ctgaattgaa acgattcagc ttctatgaga
    13801 aaaaaagcgc caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaagaaa
    13861 gcataatact tctgtttcag cacgcaagga aagccgtaaa gttatgagca aaagtttgat
    13921 gtgctgcaca attttttgtc atttttccta attgctgatg ggaaaatccg ttatcagcaa
    13981 tttcatttca gcagcgtcag cagccaatca gcaacagtga aatggcattt tgattattcg
    14041 tggcgctttt gatttcgctg tctgtttatt ttctgacaag cagcgtaaac tccgcgtctt
    14101 cctcttccag tgatcgacca gcatgcataa ctcccccgca gtctccagcg cgaaatcgtt
    14161 tgacctgacc tcgacggcgt ttttaatcgt tgcctttctc accggtattg cgggcgctct
    14221 gcaaaccccg acactcagta tttttcttac cgatgaagta catgcccgtc cggcgatggt
    14281 gggattcttc tttaccggca gcgctgtcat tgggattctg gtaagtcagt ttctcgccgg
    14341 gcgctctgat aagcgcggcg atcgcaaatc gctgattgtc ttttgctgcc tgttaggcgt
    14401 gctggcctgc accctttttg cctggaatcg caactacttt gttttgctat tcgttggcgt
    14461 ctttcttagc agctttggct cgaccgctaa cccgcaaatg tttgcccttg cccgtgaaca
    14521 tgccgacaaa accggacgtg aggcggtgat gttcagctct tttttacgcg ctcaggtttc
    14581 actggcatgg gtcattggcc caccgctggc ttatgcgtta gcgatgggtt tcagctttac
    14641 ggtaatgtat ctgagcgcag cggtagcgtt tattgtttgc ggcgtgatgg tgtggctgtt
    14701 tttaccgtcg atgcaaaaag agcttccgct ggcgaccggc acggttgaag cgccgcgccg
    14761 taaccgtcgc gatacgctac tgctgtttgt catttgtaca ttaatgtggg gctcgaacag
    14821 cctgtacatc atcaacatgc cgctatttat tatcaacgaa ctgcatcttc ccgagaaact
    14881 ggccggtgtg atgatgggga ccgccgccgg gctggaaatc ccgacgatgt tgattgccgg
    14941 atatttcgcc aaacgtctgg gtaagcgttt cttaatgcgc gttgctgccg tgggtggcgt
    15001 ctgtttttac gcaggaatgc tgatggcgca ttcacctgtc attctgttgg gcttgcagct
    15061 gctaaatgct atttttattg gcattctggg cggcatcggg atgctctatt ttcaggatct
    15121 gatgcccggt caggcgggtt cagccaccac gctctatacc aacacttcgc gcgtgggctg
    15181 gatcatcgca ggatcagtgg cgggcatcgt cgccgagatc tggaattatc acgctgtgtt
    15241 ctggtttgcg atggtgatga ttatcgccac tctgttttgc ttactgcgga ttaaagatgt
    15301 ttaaggcgcg gtcagcattt ccagatcgat aagccaggtc atggcctgct gacgcgagtt
    15361 accgcacatt tcagcgctgg cttgtaagcc tgcacagact ttcggtcgca gtggcgaggt
    15421 gaaaatttta cagcgttgct gttcatcaag ctgaatgcag ggcgtattgg cgggcttgcc
    15481 atctggcata ccgggaatag ggctggaaat tgaaggggca gtgcaacacg ccccgcaacc
    15541 cggacggcat tccatacgtt gttctcttaa ggttaaccac cggattgatg agcgcgcaca
    15601 gtaccacctt ttttgcacca gcaaaagtgc gaataccact tgcccgaaag gcccgtcgcg
    15661 agtactttgt cgcgatattt ttgacatttt cgactacagg aatttttcga tgccaagagc
    15721 gaacgaaatt aaaaaaggta tggtactgaa ttacaacggc aaactgctgc tggttaagga
    15781 tattgatatt cagtcgccca ctgcccgcgg tgccgctacg ctgtacaaaa tgcgtttttc
    15841 tgatgtccgt accgggctga aagtagaaga gcgtttcaaa ggtgatgata tcgttgacac
    15901 cgtgacgctg acccgccgtt acgttgactt ctcctatgtc gatggcaacg aatatgtctt
    15961 tatggataaa gaagactata ccccgtatac cttcaccaaa gatcagattg aagaagagtt
    16021 gctgtttatg ccagagggcg gcatgccgga catgcaggtg ctgacctggg acggtcaact
    16081 gctggcgctt gagctgccgc agaccgttga tctggaaatc gttgaaaccg caccaggcat
    16141 caaaggggca tctgccagcg cccgtaacaa accggcgaca ttgagcactg gtctggtgat
    16201 tcaggtacca gaatacttaa gcccgggcga aaaaattcgt atccatatcg aagaacgccg
    16261 ttatatgggg cgtgctgact aacttcagcc gcatgcagaa aagggatagc tcaggctgtc
    16321 ccttttttaa tttattatac caatcttcta ttttgcgctt cctgaacacc ttacgccctc
    16381 aattttcact cgttgattga tcgccctcac acttcatcgc attaacaatc cagaccaatt
    16441 tcaattgctg tcatataact ttacactgtc attgttaatt tatcgttact aagacgtgac
    16501 tcctatgaag acaattgcct ccgttacgct cccgcatcat gtacacgctc cacgctacga
    16561 tcgccagcag ttgcaatcac gtatcgttca ttttggcttt ggcgcctttc accgcgctca
    16621 tcaggcgtta ctgaccgatc gtgtgctgaa tgcccagggc ggcgactggg ggatctgtga
    16681 aatcagcttg ttcagcggtg atcaactgat gagccagctc cgcgcacaga accatttata
    16741 taccgtgctg gagaaaggtg cggacggcaa tcaggtgata attgtcggtg ccgttcacga
    16801 atgccttaat gcaaaactgg attccttagc ggcaattatt gagaaatttt gcgagccaca
    16861 ggtggcaatt gtttccctga cgattaccga aaaaggctat tgtattgacc cggccaccgg
    16921 tgcactcgac accagtaatc cgcggattat tcacgatcta caaacccctg aagaacctca
    16981 ctccgcaccg ggtattctcg tcgaagcact gaaacgccgc cgtgagcgcg gccttacacc
    17041 gtttaccgtg ctctcctgcg acaatattcc cgacaatggt catgtggtga aaaacgcggt
    17101 gctgggaatg gcagaaaaac gttcgccaga actcgccggg tggataaaag agcacgtcag
    17161 ttttccggga accatggtcg accgcattgt tccggctgca accgacgaat cactggtgga
    17221 aatcagccag catctggggg tgaatgatcc ctgcgcgatt agctgcgaac cgtttatcca
    17281 gtgggtggtg gaagataact tcgtcgctgg gcgtcctgcc tgggaagtcg caggtgtaca
    17341 aatggtgaat gatgtcctgc catgggaaga gatgaaactg cggatgctta atggcagcca
    17401 ctcttttctc gcttatctgg gttacctctc aggattcgcc catatcagtg attgcatgca
    17461 ggatcgcgca tttcgccatg ccgccagaac attaatgctg gatgagcaag cgccgacact
    17521 gcaaattaaa gatgtcgatt taacacaata tgcggataag ttaattgcac gttttgctaa
    17581 tccggcgctg aaacataaga cctggcaaat cgcgatggat ggcagccaga aattaccgca
    17641 acgcatgctg gcaggtattc gcatacatca ggggcgcgaa acggactggt cgttgctggc
    17701 attaggcgtt gcaggctgga tgcgttacgt cagcggcgtt gatgatgccg gaaatgccat
    17761 tgatgttcgc gatccgctta gcgataaaat tcgcgaactt gttgcgggca gcagcagtga
    17821 acaacgcgta accgccctgc tttccctgcg tgaagttttc ggtgatgatc tgccagataa
    17881 cccgcatttt gtgcaggcca tcgaacaagc ctggcaacaa atcgtacaat tcggcgcaca
    17941 tcaggcgcta ttaaacaccc tcaaaattta acgatttctg cggttaaagc ggatgaagct
    18001 caccttcgtc cgctctcccc ttctcttttc tgcttttttt agccaggatt aacgctcagt
    18061 taacttacca gagtattgcg gagccgttgt gaccaggacc aacctcatca ccggttttct
    18121 cggcagcggg aaaaccacgt cgattcttca tctgttagcc cataaagatc ccaacgaaaa
    18181 atgggcggta ctggttaatg aatttgggga agtcggaatt gatggtgctt tgctcgccga
    18241 tagcggcgca ttgctgaaag agatccccgg cggctgcatg tgctgcgtta atggtttacc
    18301 catgcaggta gggttgaata ccttactgcg tcagggaaaa ccagaccgct tgttgataga
    18361 gccgaccggg ctgggccatc cgaaacagat cctcgatctg ttaaccgcac cagtctatga
    18421 accgtggata gatctgcgcg ccaccttgtg cattctcgat ccgcgcttgc tgctggacga
    18481 aaaaagcgcc agcaatgaaa acttccgtga ccagctggct gccgcagaca tcattgtcgc
    18541 caataaatcc gaccgtgcga cgcccgaaag tgagcaagcg ctacagcgtt ggtggcagca
    18601 aaatggtggc gatcgacaat taattcacag tgagcatggg aaagttgacg gtcatcttct
    18661 ggatttgccg cgtcgcaatt tagccgagtt gcccgccagc gccgcgcatt ctcatcagca
    18721 tgtcgtgaaa aaagggttag cagcgttaag cctgccagag catcaacgct ggcgtcgcag
    18781 tctgaacagc gggcaaggat atcaggcctg cggctggata ttcgacgctg atacggtatt
    18841 cgacaccatt ggcattctgg aatgggcgcg acttgcaccg gtggaacgcg tcaaaggcgt
    18901 gctgcgtatt cccgaagggc tggtacgaat caaccgtcag ggcgatgacc tgcacattga
    18961 aacgcaaaac gttgcgccac cggacagccg tattgagctg atttccagca gcgaagctga
    19021 ctggaatgct ttgcagagcg cgctgttgaa gcttcgttta gcgactaccg cgtaaggttg
    19081 cctgcgtttt tcagtaagat aattagagaa aatatgatta aaaatttgcc gcaaatagtg
    19141 ttgttgaata ttgtcggcct cgcgctgttt ctttcctggt atattcccgt taatcatgga
    19201 ttctggttgc cgattgatgc ggatattttt tatttcttta atcagaaact ggtcgaaagt
    19261 aaggcctttt tgtggctggt tgcattgacc aacaatcgcg ctttcgacgg ttgttctctg
    19321 ctggcgatgg gaatgttgat gctgagtttc tggctgaaag aaaacgcccc tggcagacga
    19381 cgtatcgtga ttatgggtct ggtcatgcta ttaactgcag tggtattaaa ccagctgggt
    19441 caggcattaa ttcctgtaaa acgggccagc ccaacattga cttttaccga tattaaccgc
    19501 gtcagcgaac tgctctctgt tcccacgaaa gatgcctcac gagatagctt tcccggcgat
    19561 cacggcatga tgctgcttat tttttcggca ttcatgtggc gttatttcgg caaagttgca
    19621 ggccttatcg cccttattat ttttgtggtt tttgcatttc ccagagtaat gattggcgca
    19681 cactggttta ctgacatcat tgtcggttcg atgaccgtga tattgatcgg tttgccctgg
    19741 gtgttgctga cgccattaag tgatcgatta atcacctttt ttgacaaatc actaccggga
    19801 aaaaacaaac atttccaaaa caaataactc acagtaatta acatcatcag gcttattttt
    19861 atagtgagga taatcctgat gatgcgcacc gtgctttcat ccatcgcgcg caaaaatcat
    19921 tctctaagta aatgaatgga ttgcatgcgt ttcactcaat cgtactttaa ttgaccaatc
    19981 ccgcttatta actttctgta tcactttttc ttataaaaaa tcatgtaaaa ccgctcgcca
    20041 agaccgcacc aatcgggtaa tctcgaactc gttttgcctc ggcggtagat tatcctcaca
    20101 gcatataatt ttgtgcgtta gtccacagat ttggccttaa ggaattgttt caacatgccc
    20161 aggtaattag tctcgtgtcg cttggcattt ttttataacg atatttgtcg ttaaggactt
    20221 caagggaaaa caaacaacat ggtcaaatct caaccgattt tgagatatat cttgcgcggg
    20281 atccccgcga ttgcagtagc ggttctgctt tctgcatgta gtgcaaataa caccgcaaag
    20341 aatatgcatc ctgagacacg tgcagtgggt agtgaaacat catcactgca agcttctcag
    20401 gatgaatttg aaaacctggt tcgtaatgtc gacgtaaaat cgcgaattat ggatcagtat
    20461 gctgactgga aaggcgtacg ttatcgtctg ggcggcagca ctaaaaaagg tatcgattgt
    20521 tctggttttg tacaacgtac attccgtgag caatttggct tagaacttcc gcgttcgact
    20581 tacgaacagc aggaaatggg taaatctgtt tcccgcagta atttgcgtac gggtgattta
    20641 gttctgttcc gtgccggttc aacgggacgc catgtcggta tttatatcgg caacaatcag
    20701 tttgtccatg cttccaccag cagtggtgtt attatttcca gcatgaatga accgtactgg
    20761 aagaagcgtt acaacgaagc acgccgggtt ctcagccgca gctaataaac cgtttggatg
    20821 caatcccttg gctatcctga cgagttaact gaaagcactg cttaggcagt gcttttttgt
    20881 tttcattcat cagagaaaat gatgtttccg cgtcttgatc caggctatag tccggtcatt
    20941 gttatctttt aaatgttgtc gtaatttcag gaaattaacg gaatcatgtt catacgcgct
    21001 cccaattctg gacgtaagct cctgcttacc tgcattgttg caggcgtgat gattgcgata
    21061 ctggtgagtt accttcaatt tttagtggcc tggcataagc acgaagtcaa atacgacaca
    21121 ctgattatcg acgtacaaaa gtatctcgat acctattttg ccgacctgaa atccactact
    21181 gaccggcttc agccgctgac cttagatacc tgccagcagg ctaaccccga actgaccgct
    21241 cgcgcggcgt ttagcatgaa tgtccgtacg tttgtgctgg tgaaagataa aaaaacattc
    21301 tgttcatctg cgactggtga gatggacatt ccactcaatg aattgattcc ggcgctcgac
    21361 attaataaaa acgtcgatat ggcgatctta cccggcacgc cgatggtgcc gaacaaaccc
    21421 gcaatcgtca tctggtatcg caaccctttg ctgaaaaata gcggtgtgtt tgccgctctg
    21481 aatctcaacc tgacgccttc tctcttttat agttcacggc aggaagatta cgatggcgtc
    21541 gccctcatta ttggcaatac tgcgctatct accttttctt cacgtttgat gaacgttaat
    21601 gaattaaccg acatgccggt ccgtgaaact aaaattgcgg gcattcctct gaccgttcgg
    21661 ctttatgcgg atgactggac atggaacgat gtgtggtacg catttttact gggcggcatg
    21721 agtggaactg tcgttggcct gctctgctat tacctgatga gcgtacgtat gcgccccggc
    21781 agagaaatca tgaccgccat caagcgcgaa caattttacg tggcgtatca accggtggtg
    21841 gatacacaag ctttgcgagt aacgggcctg gaagtactgc tacgctggcg gcatcctgtc
    21901 gcgggagaaa ttcccccgga tgccttcatt aactttgccg aatcgcaaaa gatgattgtg
    21961 ccgctgactc agcacctgtt tgagttaatt gcccgcgatg ccgcagaatt agaaaaagtg
    22021 ctgccagtag gcgtcaaatt tggtattaac attgcgccgg accatctgca cagcgaaagc
    22081 tttaaagcag atatccagaa actgctcact tccctacccg cacaccattt ccagattgtg
    22141 ctggaaatta ccgagcgcga tatgctgaaa gagcaagaag ccacacaact cttcgcctgg
    22201 ctgcactcgg tcggcgtaga aattgctatt gatgacttcg gcaccgggca cagcgcgctt
    22261 atctatcttg agcgttttac gctcgattat ctgaaaattg accgtggatt tatcaacgcc
    22321 atcggtacgg aaacgatcac ttcacccgta cttgacgcgg tgctgacgct ggcgaaacgt
    22381 ctcaatatgc tgacagttgc tgaaggggtc gaaacgccgg aacaagcgcg atggctaagc
    22441 gaacgcggcg ttaatttcat gcaaggctac tggattagtc gcccgttacc gctggacgat
    22501 tttgtccgct ggctgaagaa accgtatacg ccgcagtggt aaggtgtgct tacgtccctt
    22561 attattcata gtgaaagcat gccggattgc ggctaatgat gagtaaaagg aaatccgttg
    22621 cagatgattg tgcgcatact gctgctgttt atcgctctgt tcacctttgg tgtgcaggcg
    22681 caggctatca aggaaagcta tgcctttgcc gtgctgggcg aaccccggta cgcgtttaat
    22741 ttcaaccatt ttgattatgt gaaccccgcc gcgccaaaag gtgggcagat aacgttgtca
    22801 gccctcggca ccttcgatac tttcaaccgc tatgcactgc gcggcaaccc gggcgcacgc
    22861 accgagcagc tgtacgacac gctatttacg acttccgatg acgaaccagg cagttattac
    22921 ccgctgattg ctgaaagcgc acgctatgct gacgattatt cctgggtgga ggtcgctatt
    22981 aatccgcgcg cccgttttca tgatggttcg cccattactg cccgcgatgt agagtttact
    23041 tttcaaaaat ttatgaccga aggcgtgccg caatttcgtc tggtctacaa aggcaccacc
    23101 gtcaaagcca ttgcgccgtt aaccgtgcgc attgagttag ctaaacccgg caaagaagat
    23161 atgttgagtc tgttttcgct gccggtattt ccagaaaagt actggaagga tcacaaactt
    23221 agcgacccgc tcgccacgcc tccgcttgcc agtggtccgt accgcattac gtcctggaaa
    23281 atggggcaaa atattgtcta ttcccgcgtg aaagattact gggcagcaaa cttaccggta
    23341 aaccgcggac gctggaattt cgacaccatt cgctacgatt attacctcga tgataatgtc
    23401 gcctttgaag cgtttaaagc aggtgccttt gatttgcgta tggaaaacga cgccaaaaac
    23461 tgggccacgc gttataccgg taaaaatttc gataaaaaat acatcatcaa agatgagcaa
    23521 aagaacgaat cagcccagga tacgcgctgg ctggcgttta atatccaacg tccagtattc
    23581 agcgatcgcc gggtgcggga agcaatcact ctcgcctttg actttgaatg gatgaacaag
    23641 gcgttgtttt acaatgcctg gagtcgcacg aacagttatt ttcagaatac cgaatacgcg
    23701 gccagaaatt accccgacgc cgcggagctg gtgcttctgg caccaatgaa aaaagatcta
    23761 ccgccagaag tcttcacaca aatctaccag ccgccggtat ccaaaggcga tggctacgat
    23821 cgtgacaacc tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt gctgaagggt
    23881 cagcaacgcg ttaatgccac aacgggtcag ccactcagct ttgaattatt gcttcccgca
    23941 agcagcaata gtcagtgggt attgccgttc cagcacagcc tgcaacggct gggtatcaac
    24001 atggacattc gcaaggtgga taactcgcaa atcactaacc gcatgcgcag tcgcgactat
    24061 gacatgatgc cgcgcgtatg gcgggcgatg ccgtggccca gttccgattt acagatttcc
    24121 tggtcatcgg aatatatcaa ttccacttat aatgcccccg gcgtgcaaag cccggttatc
    24181 gactcgctga tcaaccaaat tattgccgcg cagggaaata aagaaaaatt actgccgttg
    24241 gggcgagcac tggatcgcgt attaacgtgg aattattaca tgctgccaat gtggtacatg
    24301 gcggaagacc gtctcgcctg gtgggataaa ttctcccagc cggccgtgcg ccccatctat
    24361 agcctcggta tcgatacctg gtggtatgac gtcaataaag cggccaaact gccgtccgcc
    24421 agcaaacagg gagagtagat gggcgcttac ctgattcgcc gtctgttgct ggtgatccca
    24481 acattatggg cgattatcac catcaacttt ttcatcgtgc aaattgcgcc tggcggtccg
    24541 gtcgaccagg ccatcgccgc cattgagttt ggtaatgccg gagtattacc cggcgcaggc
    24601 ggtgaaggtg ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag cgacagtaag
    24661 taccgtggcg gacgcggatt agatccagaa gtgatcgctg agatcactca tcgctacggt
    24721 tttgataagc cgatccacga acgttacttc aaaatgctct gggactacat ccgctttgat
    24781 tttggcgata gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga cagtctgccg
    24841 gtttccatca ccctcggatt gtggagcacg ctgattatct atctggtgtc gattccgtta
    24901 ggcattcgca aagctgttta taatgggagc cgctttgacg tctggagtag cgcatttatc
    24961 atcatcggct acgccattcc ggcctttttg tttgccatcc tgctgattgt cttcttcgcg
    25021 ggcggcagct atttcgacct gttccctcta cgcggcctgg tttccgctaa ctttgattcg
    25081 ctgccgtggt atcagaaaat caccgattat ctgtggcata tcacgctgcc ggtgctggcg
    25141 acagtgattg gtggctttgc ggcgctgacc atgctgacaa aaaactcatt ccttgatgaa
    25201 gtgcgcaagc aatacgtggt gaccgcgcgt gcgaaagggg taagtgaaaa aaatattctc
    25261 tggaaacatg tgttccgcaa cgccatgctg ctggtgattg ccggttttcc ggcgacgttt
    25321 atcagcatgt tttttaccgg ctcgctgctg attgaggtga tgttttcact caatggtctg
    25381 ggcttactgg gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt tggtaccttg
    25441 tatattttca ccctgattgg cctgctgctg aatattgtca gtgatatcag ctatacgctg
    25501 gtcgatccgc gtatagattt tgagggacgt taatgtcgcg actcagcccc gtcaatcagg
    25561 cccgttgggc gcgttttcgc cataaccgtc gcggctactg gtcgttatgg attttcctcg
    25621 ttttgtttgg tttgagtttg tgttctgaac ttatcgccaa cgataaaccg ttgctggtac
    25681 gttatgacgg cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc gattttggcg
    25741 gcccgctggc aagtcaggct gattatcagg acccgtggct gaaacaacgg ctggaaaata
    25801 acggctgggt actgtgggca ccgattcgct ttggtgctac cagtatcaac tttgctaccg
    25861 ataagccctt cccttctcca ccctcccggc aaaactggct gggaacggat gccaacggcg
    25921 gcgatgtgct ggcacgtatt ctctatggca cgcggatctc ggttctgttt ggcctgatgc
    25981 tgactctctg ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc tattacggcg
    26041 gtaaagtcga tctctggggg caacgcttta ttgaagtatg gtcggggatg ccgacgctgt
    26101 ttttgattat tttactttcc agcgtcgtac agcctaactt ctggtggctg ctggcaatta
    26161 ctgtcttgtt tggctggatg agtctggtcg gcgtggtgcg ggcggagttt ttacgcaccc
    26221 gtaatttcga ctacattcgt gcggcgcagg cgcttggcgt cagcgatcgc agtatcatcc
    26281 tgcgtcatat gttgcctaat gccatggtcg cgaccctcac ctttttaccg tttattttat
    26341 gtagttcgat aaccaccctg acctcactcg atttcctcgg cttcggtctg ccgctcggtt
    26401 caccgtcact cggtgaactg ctgttacaag ggaaaaataa ccttcaggct ccgtggcttg
    26461 ggatcaccgc cttcttgtcg gtggcgatat tattgtcttt gctgatcttt attggtgaag
    26521 ccgtccgcga cgcatttgat cctaataagg cggtgtagca tgacgcaaac tctgttagcg
    26581 attgaagatt tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt agtcaatgat
    26641 gtttcactac agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc aggttcaggc
    26701 aaaagcgtta ccgcgctgtc aattttacgc ctgctccctt ccccgccggt tgaatatctc
    26761 tccggcgata ttcgttttcg tggcgaatcg ctgcttcatg ccagcgatca aacgttgcgc
    26821 ggtgtacgcg gtaataagat cgccatgatt tttcaagagc cgatggtgtc gttaaatcca
    26881 ttgcataccc tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg gatgcgtcgg
    26941 gaagcggctc gaggcgaaat tcttaactgc cttgatcgcg ttggtatccg ccaggcggca
    27001 aaacggctga cagattatcc gcatcagctc tccggcggcg aacgccagcg ggtgatgatt
    27061 gcgatggcgc tgttaacgcg accggaatta ttaattgccg atgaaccgac caccgcactg
    27121 gacgtctctg tccaggcgca gattttacag ctgttgcgcg aactgcaagg cgagctgaat
    27181 atgggcatgc tgtttattac tcataacctc agcattgtca gaaaactggc ccaccgcgtg
    27241 gcggtaatgc aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct atttgcctca
    27301 cccactcatc cttacacaca aaagctactc aacagtgaac cgtcaggcga cccggtgccg
    27361 ttgccagaac ctgcctcaac gttgctggat gttgaacagc ttcaggttgc cttccccatt
    27421 cgcaaaggga ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa catcagtttt
    27481 acgctacgag cgggtgaaac actgggttta gtgggcgagt ccggttccgg gaaaagtacg
    27541 acgggactgg cgctgctgcg actgattaat tctcagggca gcatcatctt tgacggtcag
    27601 ccactgcaaa atttaaatcg ccgccagctg ttacctattc gtcatcgcat tcaggtggta
    27661 tttcaggatc caaactcctc gctcaaccca cgactcaacg ttttgcagat tattgaggaa
    27721 ggcttacggg ttcaccagcc gacgctttct gccgcacaac gcgaacaaca agtgatagcc
    27781 gtgatgcatg aagtgggatt agatcctgaa acacgccacc gttatccggc ggagttctct
    27841 ggtggtcagc gacaacgtat tgcgattgcc agggcattaa ttcttaagcc ctcgctgatc
    27901 atacttgatg aaccgacatc atcactcgac aaaacggtac aggcgcaaat attgacgcta
    27961 ttgaaatcat tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca cgatttgcac
    28021 gttgtccgcg cgttatgtca tcaggttatc gtactgcgac aaggggaagt agtggaacaa
    28081 ggaccgtgcg cgcgcgtgtt tgccacaccg cagcaggagt atacgcgtca gctactggcg
    28141 ttgagctgac gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg ccacaccaaa
    28201 atttttcaat cggcatgttg cagcaaactc gtcctggcga tttacaaaca ggcacggctc
    28261 accttcacac tccagcaccg aacatggaac ttcgatgtcg cttaacgcct ggcttagttt
    28321 atacatcacc gaccatgcat tatcaccatc cgggctaagc aatttaagag ccaccaggct
    28381 gttgtcaccg cacggtccat tttgcggaat cggttcaacc ttcgaacctg cgaaaccagc
    28441 agaccagcga tagttctgcg gcagtcgatg gacgattgag agttgtaatg aagtcactta
    28501 ttttccccgg aagcacattc acttcacaat ttgtttacat caattttaac acatcatcaa
    28561 caatccgtct ttatacagat cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc
    28621 tgatttaagt aatatctgcg ggcgtggtca cgtttgcggg gctatatgta cccgtttctg
    28681 cgatctaact caaccttttt aactacaatg atgtgacttt ttacataaat tgattttaca
    28741 taaaataaac atatatcggg ggaatgatag atttgtggtt gacatgcatc aacaaaggaa
    28801 gccttttagc ttcctcgttg tgcaatagat caccgttttt tcggtcgact ggcgtacaga
    28861 cagaagagaa tggagctgct tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag
    28921 ttaaaggtcg caagagaaag caatgcgcca acaattgccc ctatgccaaa acggaaggtt
    28981 cctgccagcg aagatgccgt tcccgccata tggggaaact catcaagaat gaccgccatc
    29041 gcattggatg acaccatcga cacgcagccc acaaacgccg caacgccaac caccagcgac
    29101 cagaatccca gccccagcag cgcactgatg accatccacg ctgccataat aaattgtatc
    29161 cacaaccccg agcggaacat atttaacgcg ccaatgcggc gaacgaagcg gctgttaaaa
    29221 atggtcatca cgaacaggaa aacaatgttt agtgcaaagt aataaccaaa gttttccggc
    29281 gcgacgtggt taatttcaat gtagacaaac ggtccggcgc ttaagaacga gaacatcccg
    29341 gcaaagctga aaccactggc aagcatataa ctcaggacac gcttatgacg gaacagcgcc
    29401 gcaaagttac caatagtggt acgaatgtga aaaggctggc gacgctccgg tggtaaggtt
    29461 tctttaatca ggaagaaaat cattgccgaa gccagaatcg ccgctaatgc caggatccag
    29521 aagatgtaat gccagctcag ccacaccagc acccaaccgc cgacaatcgg tgccatcagc
    29581 ggtgcaatag tggtcaccag catgacaaac gacatcatcc gcgagaactc ttctttcggg
    29641 taaatatcgc gcatcagggc gttaatgacc acgctggccg cagccgccgc cagcccgtgg
    29701 aagaaacgca tcacaatcag ctgatcgatg gtgtttgcca acgcacacgc caccgcggcg
    29761 gcggcaaaca ccagcgtacc gccgagcacc accggcttac gcccgaagct gtctgccatc
    29821 ggcccgtaga ttaactgccc caacgcaaag cccagaatat aagtactgag ggtcatctgc
    29881 gtactgcccg caggtacgcc aaactgcgct gaaattaccg gtagcgcggg cagatacata
    29941 tcaatcgaca gcggcatcaa catggccagc aggccaagga taaaaacaat agcaaacgac
    30001 gaatgctgtc gggtggtcac aacgggctcc tgaaagtcat ggaaaagtta gacgacgctg
    30061 gcaatttctt cttcagttaa cggacgatat tcaccggggg ctaaatcagc atccagcgta
    30121 ataccgccaa tacgttcacg atgcagctca accacgtggt tacccacggc ggcgaacatg
    30181 cgtttcacct gatgataacg cccttcgctg atggtcagac gaacctgcgt tggggtaatc
    30241 acttccagca ccgcaggctt agtgagatct ttttcgttat gcagctgcac gcctttagca
    30301 aattgctctg ccgtatcgtc agctacaggt gattccagtg tcaccagata ggtcttctcg
    30361 caatgatggc gcggagaagt aatgcggtgc gaccactgac catcatcagt catcagcacc
    30421 agaccggtgg tatcgatatc cagacgtcct gcggcgtgca gtttccaggc taccggttca
    30481 tccagaaaat agagcaccgt tgggtgatca gggtcgtccg tggagcaaac atagccctga
    30541 ggcttattga gcatgaagta acgtggacca tgttgctgcg ccagcgggtt gccatcgtaa
    30601 gcgacatcat gttcaggaag cagtttgaac gctgcattac gaacaatttc gccatcgacg
    30661 gtgacacgat tgccgcggat ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt
    30721 gcgataaatt tatcaagtcg catgtgtgtg attttgcctg taaaaacgga ggtcgggcat
    30781 tgacccgaaa atctgaactg ttgtctgccc agtatagcgg tctaataaca tccctcaagg
    30841 gaaaaagatc catggcatac tattagcaga ataatctacc tacgcgagac catgattttt
    30901 acactccgcc catatcagca agaagccgtg gatgccacgc tcaaccattt tcgtcgtcat
    30961 aaaacccctg ccgttatcgt gctgcccacc ggcgcaggta aaagcctggt gattgccgaa
    31021 ctggcacggc tggcgcgtgg tcgtgttctg gtgctggcac acgtcaaaga actggtggcg
    31081 caaaaccatg caaagtatca ggcgctgggg ctggaagccg atatttttgc cgctgggtta
    31141 aagcgcaaag agagccacgg taaagtggta tttggcagcg tgcagtcggt cgctcgtaat
    31201 cttgatgcct ttcagggtga attttcgctg ttgattgtcg atgaatgcca ccgtattggt
    31261 gacgatgaag agagccagta tcagcaaatc ctcactcacc tgactaaagt gaatccccac
    31321 ttacgcctgc tggggctgac tgccacgcct tttcgattgg gcaaaggctg gatctaccag
    31381 ttccattatc acggcatggt acgcggcgat gagaaagccc ttttccgtga ctgcatttat
    31441 gagctgccgc tgcgttatat gattaaacac ggctatctga cgccgccaga acgactggat
    31501 atgccagtag tgcaatacga tttcagccgc ttgcaggcac agagtaacgg gctgttcagc
    31561 gaagctgatc tcaaccgtga gctgaaaaaa cagcaacgca ttacgccaca tatcatcagc
    31621 cagatcatgg agttcgccga aaagcgcaaa ggggtaatga ttttcgccgc caccgttgaa
    31681 cacgcaaaag agattgtggg attactgcct gccgaagatg ctgcactgat tactggcgac
    31741 acccccggcg ctgagcgcga tgtgttaatt gaagatttta aagcccagcg ttttcgctat
    31801 ctggtcaacg tcgcagtact gaccaccgga tttgacgccc cgcacgtcga tcttatcgcc
    31861 attctgcgcc ctaccgaatc agtgagtctt taccaacaaa ttgtcgggcg cggtctgcgt
    31921 ctcgctccgg gcaagactga ttgtttaatt cttgattatg cgggtaatcc tcacgatctc
    31981 tacgcgccgg aagttggtac accgaaaggc aaaagtgaca acgttccggt acaggttttc
    32041 tgccctgcct gcggttttgc caacaccttt tgggggaaaa cgaccgccga cgggacattg
    32101 attgaacact ttggtcgccg ctgtcaggga tggtttgaag atgacgacgg tcatcgcgaa
    32161 cagtgtgact tccgtttccg ttttaaaaat tgcccgcaat gtaacgcaga aaatgatatt
    32221 gccgctcgcc gctgccgcga atgtgacacg gtactggttg atccggatga tatgttaaaa
    32281 gcggcgctac gactgaaaga tgcgctggta ttacgctgta gcggcatgtc tttgcagcat
    32341 gggcacgacg agaaaggcga atggttgaaa atcacctatt acgatgaaga cggcgcggat
    32401 gtgagtgagc gtttccgtct gcaaacgccc gcccagcgaa ctgccttcga gcagcttttt
    32461 atccgcccgc atacgcgcac accgggcatc cctctgcgct ggatcaccgc cgccgatatc
    32521 ctcgcccagc aagccttact gcgacatccg gattttgtcg tcgcccgcat gaaaggtcag
    32581 tactggcagg tgcgtgaaaa agtgttcgat tacgaaggtc gttttcgtcg ggcgcacgaa
    32641 ttacgcggtt aatggtagtt ttgattgatg tataaggcga ttgagtatag aatctcgccc
    32701 gcttttgcat acgcaaagca gatcacttac ctgttgctgg gtcgcctgta gcaggattaa
    32761 tttaagagag aaagaaatgt ttactatcaa cgcagaagta cgtaaagagc agggtaaggg
    32821 tgcgagccgc cgcctgcgtg ccgctaacaa gttcccggca atcatctacg gtggcaaaga
    32881 agcaccgctg gctatcgagc tggatcacga caaagtcatg aacatgcaag ctaaagctga
    32941 attctacagc gaagttctga ccatcgttgt tgacggtaaa gaaatcaaag ttaaagctca
    33001 ggacgtacag cgtcacccgt acaaaccgaa gctgcagcac atcgacttcg ttcgcgctta
    33061 attgctgaat aagttgtaaa aaaccccgct ccggcggggt tttttgtatc tgcagattat
    33121 gcctgattac ggtattgcta ttttttgcag gccagataag gcgttcacgc cgcatctggc
    33181 attcagaaca caaaaccgat taattgccgc cagacgtccg gcgctgcaat tggtcgcgca
    33241 aattcggcgg cgtgccttta atggtcaagg tatcggttgc tggatcccag aagatgcgtt
    33301 cacctaacag catcgcatca aagttaatag tcaagccacc gccgctaccg gcaaacttcg
    33361 tcaactgacg cagtgtgcta cggtctgccg ggaagctctc ttccagttcg tagccttttt
    33421 cagccgcaaa ctccgtgaag ctcacttcgc ttacgcctgc cagctctttc gacagcgatt
    33481 ccagttcgat ctcttcgcct gcctgcagct gctcattaca gtagctataa acctgctggc
    33541 gcacgttctg tcgctctgct ttatccagtt gtgcttcggc agtgaaatca tcaaccgctt
    33601 gcaacagccc acggttttgc gctttcgcgt tcaatccttc gctggccccg aggaaatcca
    33661 taaagaaatc cgccactttg cgccctactc gccctttcag gaaagtgaga tagcgggtgg
    33721 actctggatt ggtttcccat tcggttaaat cgatacgcgc aacaatatcc gcatgattga
    33781 tatcaagata atgggttggg ttgatgtcga gattttcgtt aacgcgcata ctgctcaggt
    33841 tgctcagaac tgccaccagc agatactcca ccgccagata acggtagtgg caaaacagca
    33901 cgaatccacc gtcagcaaaa ggatatttcg ctaattcgtc acgcaggcgc ccggttgccg
    33961 cgcggctaaa tgccagaaag tcctcttcgc cctgacgctg taagcgcagc gtctgcgcca
    34021 gttcactctc ttcgctaaac aagccgtaag ctttattttt ggcgctatag acccgatgca
    34081 gttctgccac catctcaacg acggtttctg tcggttccag caatgaatcg cgcaacacca
    34141 gctcaaggtt ctgctcatca cgcttgataa gctggtgcag ggcaatctgg ttgatatcca
    34201 gactcatgat aaactctcct ttaagaccgg gcggtattca accaccgccg gttaagcgac
    34261 gcaataaaag atggcggtca ttttacagaa ggcagcgtaa cgagaatgat ttaacgcata
    34321 aaaaagcaga aaaaaaaccg ttgctacggt aatatgttgc cctttcatca actaactgat
    34381 ttcgatttat gccacaaatt tcccgctaca gtgatgaaca ggttgaacaa ttgctcgcag
    34441 agctgctcaa cgtactggaa aaacataagg ctccgaccga cctttccctg atggtgttag
    34501 gcaacatggt cactaacctt atcaacacca gcattgcccc ggcccaacgc caggcaattg
    34561 ccaactcttt tgcccgcgcc ttacagtcct ctatcaacga agacaaagcg cactaaggga
    34621 aacagataac tggttatggt aactcatcgt cagcgctacc gtgaaaaagt ctcccagatg
    34681 gtcagttggg ggcactggtt tgcactgttc aatattctgc tttcgctcgt cattggcagc
    34741 cgttacctgt ttatcgccga ctggccgaca acgcttgctg gtcgcattta ttcctacgta
    34801 agcattatcg gccatttcag cttcctggtg ttcgccacct acttgctgat cctcttcccg
    34861 ctgaccttta tcgtcggctc ccagaggctg atgaggtttt tgtccgtcat tctggcaacg
    34921 gcgggaatga cgctattact gatcgatagc gaagtcttta ctcgtttcca tctccatctt
    34981 aatcccatcg tctggcaact ggttatcaac ccagacgaaa atgagatggc gcgcgactgg
    35041 cagctgatgt tcatcagcgt gccggttatt ttattgcttg aactggtgtt tgcgacgtgg
    35101 agctggcaaa agctgcgcag cctgacgcgt cgtcgacgct tcgcgcgccc gctggccgca
    35161 ttcttattta tcgcctttat cgcctcgcat gtggtgtata tctgggccga tgccaacttc
    35221 tatcgcccga tcaccatgca gcgcgctaac ctgccgcttt cgtacccgat gacggcgcga
    35281 cgttttcttg agaagcatgg tctgcttgat gcgcaggagt atcaacgccg tcttattgag
    35341 caaggtaatc cagacgccgt ttccgtacag tatccgttaa gcgaactgcg ctatcgcgat
    35401 atgggcaccg ggcagaatgt gctgttgatt actgtcgatg gcctgaacta ctcacgcttc
    35461 gagaagcaga tgcctgcgct ggcaggtttt gctgagcaaa atatttcgtt cacgcgccat
    35521 atgagctccg gcaacactac agacaacggc atctttggcc tgttctatgg catctcgccg
    35581 agctatatgg acggcattct gtcgacccgt acgcctgcgg cattaattac tgcgcttaat
    35641 cagcaaggct atcagctggg gttattctcg tcagatggct ttaccagccc gctgtatcgc
    35701 caggcattgt tgtcagattt ctcgatgccg agcgtacgca cccaatccga cgagcagacc
    35761 gccacgcagt ggatcaactg gctgggccgc tacgcacaag aagataaccg ctggttctcg
    35821 tgggtctctt tcaatggcac taacattgac gacagcaatc agcaggcatt tgcacggaaa
    35881 tatagccggg cggcaggcaa tgtcgatgac cagatcaacc gcgtgctcaa tgcactgcgt
    35941 gattctggca aacaggacaa tacggttgtg attatcactg ccggtcgggg tattccgctg
    36001 agcgaagacg aagaaacctt tgactggtcc cacggtcatc tgcaggtgcc attagtgatt
    36061 cactggccag gcacgccggc gcagcgtatt aatgcgctga ctgatcatac cgatctgatg
    36121 acgacgctga tgcaacgcct gctacatgtc agcacacctg ccagcgaata ttcgcaaggt
    36181 caggatttgt tcaaccctca acgccgtcat tactgggtta ctgcagcgga taacgatacg
    36241 ctggcaatta ccaccccgaa aaagacgctg gtgctgaaca ataacggtaa ataccgcact
    36301 tacaacttac gtggtgaaag agtgaaagat gaaaaaccac agttaagttt gttattgcaa
    36361 gtgctgacag acgagaagcg ttttatcgct aactgattaa ttataaatca gttagcgaaa
    36421 tatcttactt gcaatcggtg tggaaaacgg tagtattagc agccacgagt cggcacgtag
    36481 cgcagcctgg tagcgcaccg tcatggggtg tcgggggtcg gaggttcaaa tcctctcgtg
    36541 ccgaccaaat cgcccaccgg atatcaccgg aaagcaccgg ataagacagg gataacccgc
    36601 taatcacttg attggcgggt ttttttatgt tttttagtca ccgaacatca ccggatttat
    36661 aagctatcag ccggattttt tagttatacg tttagttata cgggctggcg tataactaaa
    36721 gccgtataac taaaaccctg ttttttcgtg tggaatcata gacatatggc gagaaaaaca
    36781 cacccattaa ccacagtgca gatcaaagca gccagaccag cgcaaaagga gtacaccctg
    36841 caggacggcg gagggctttt tctcctggtc aaaccgtctg gatcaaaact ctggagattt
    36901 tcctactacc gaccatcgga caaaaaaaga atattgctga gttttggatc gcttgaagat
    36961 gtttccctgg ctgatgccag aaaacgccgt agcgagtaca ggacgttaat cagtgccgga
    37021 actgacccgc aggaccacga gaggcaaaaa agagagacag aggcccgaag acaagggaac
    37081 acgttcgaaa atgtggcggc ggcatggtac caggtgaaaa tcagccagaa tctggccccc
    37141 aacacgatta aagacatctg gcgttcgctg gataaatatg tattcccgtt catcggcaac
    37201 acgccaatag ataccctcac cgcccgaagg ttcgttgaag tgcttacccc catcaaggag
    37261 cgcggcaacc tggaaacact caagcgggtt ttacagcgcg ttaatgaggt aatggattac
    37321 gccgccaaca gtgggctgat tgatgccaat ccggctatga atgtgcgtaa ggcgtttccc
    37381 tcacctgtaa aaaaacatat gccaacaatc cgccccgaac agctgcccga gcttatgcag
    37441 gctttatcag tatcggcaac agaacggcag accagattac tgattgaatg gcagttactg
    37501 accgtaaccc gtcccgccga agcgtcatca acgcggtggg atgaaatcaa cctggacgcg
    37561 aagcaatgga cgatacctgc cggacgcatg aagatgcgca gggatcacgt tatcccgctt
    37621 tccggtcagg ctatggcggt gctggaggcc atgaaaccaa tcagccacca ccgcaattac
    37681 gttttcccaa gtctgaaaga cccacagcag ccgatgaaca gccagacagc taacgcagca
    37741 ttgcggcgta tgggattcgc tggcgtgctg gtgtctcatg gattacgcgc catattcagc
    37801 acagcagcga acgaggaagg attcgagccg gacgtaatag aggcggcact tgcccacgtc
    37861 gacaccaacg aagttagacg ggcatacaac cggagcaact acatagaaaa acgcatcgtg
    37921 ctgatgcgct ggtggggcga atttgtcgag gctgcggcga cgggcgtaac cctcgccagt
    37981 ggtaaaaggg gtatccgagc cgtgtagctg tacagaaaac cagtaaaaac tacgaaaacc
    38041 atgtaaaacc gtcgtataat tgcatcaaat ttaacgacaa ggccgtgaaa catgaaaccg
    38101 ttaagatgca aaaaaatatc agatgcaatt gcgacgggct gcaactggcc ctgatggttc
    38161 agcatgaatt ttggtcaacc tacgatccgg aggacagaac gacggcccca tcaaaaaaac
    38221 aggtagtaga ttttctggta tcccgtggcg cttccagaaa tctggcggta agtattgata
    38281 aggtcgtacg tccggcatct atgaagatcg gaggcaggcc caaaaaatgg cggtaacaat
    38341 cctggaagcg gcagaaatgc cgcttttttt ataattccat ttcaaatcat caatataaaa
    38401 aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg atgatttacc
    38461 gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag caatcagaaa
    38521 aattatcttt cgccaggaag taaaaaaatt atccacatca aggcagacag cacgctgcaa
    38581 agcatgatca acgccggaga atttccgcag ggttttcgcg ttggtttacg ccgtcgcgga
    38641 tggtatgagg atgatgtgtt gtcctggcag aaagaacgcg aagaggaagc acgcggaacg
    38701 gctgcttaac gggtatcaca gatatgacaa acacgaaaaa agcggccccg atatggagcc
    38761 gcctttctga acaattaacc cgctgcgcct tatgtgtatg tgatcccaaa cataagcacg
    38821 gggatgatag ccgctatc
//
LOCUS       DAZFKM010000031        38159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFKM010000031 DAZFKM010000000
VERSION     DAZFKM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(190..2211)
                     /gene="fyuA"
                     /locus_tag="ACHKAY_001740"
     CDS             complement(190..2211)
                     /gene="fyuA"
                     /locus_tag="ACHKAY_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO5700431.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            complement(2342..3907)
                     /gene="ybtE"
                     /locus_tag="ACHKAY_001741"
     CDS             complement(2342..3907)
                     /gene="ybtE"
                     /locus_tag="ACHKAY_001741"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO5700432.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            complement(3911..4714)
                     /gene="ybtT"
                     /locus_tag="ACHKAY_001742"
     CDS             complement(3911..4714)
                     /gene="ybtT"
                     /locus_tag="ACHKAY_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO5700433.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            complement(4711..5811)
                     /gene="ybtU"
                     /locus_tag="ACHKAY_001743"
     CDS             complement(4711..5811)
                     /gene="ybtU"
                     /locus_tag="ACHKAY_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO5700434.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            complement(5808..15290)
                     /gene="irp1"
                     /locus_tag="ACHKAY_001744"
     CDS             complement(5808..15290)
                     /gene="irp1"
                     /locus_tag="ACHKAY_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFO5700435.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGSVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWHTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELASYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     SLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLASHVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALSCGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAVS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRKTSAWHDAWDYWQEKLPQLPLAPELPMVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFTAHP
                     EAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGFIEG
                     LSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPGVNR
                     PDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNSALEK
                     QVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFNHSTLSD
                     FCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQAKS
                     PQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRMVLI
                     DPVCRQDFCCENRAALLRLLAEGETPLALPEHFDQQTPDSQLADFISLAKTAGMVSQN
                     LTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETELQGWINN
                     ADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            complement(15378..21485)
                     /gene="irp2"
                     /locus_tag="ACHKAY_001745"
     CDS             complement(15378..21485)
                     /gene="irp2"
                     /locus_tag="ACHKAY_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO5700436.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            complement(21676..22635)
                     /gene="ybtA"
                     /locus_tag="ACHKAY_001746"
     CDS             complement(21676..22635)
                     /gene="ybtA"
                     /locus_tag="ACHKAY_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO5700437.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGIAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            22892..24604
                     /gene="ybtP"
                     /locus_tag="ACHKAY_001747"
     CDS             22892..24604
                     /gene="ybtP"
                     /locus_tag="ACHKAY_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO5700438.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            24591..26393
                     /gene="ybtQ"
                     /locus_tag="ACHKAY_001748"
     CDS             24591..26393
                     /gene="ybtQ"
                     /locus_tag="ACHKAY_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO5700439.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAACAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            26386..27666
                     /gene="ybtX"
                     /locus_tag="ACHKAY_001749"
     CDS             26386..27666
                     /gene="ybtX"
                     /locus_tag="ACHKAY_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO5700440.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            27694..28998
                     /gene="ybtS"
                     /locus_tag="ACHKAY_001750"
     CDS             27694..28998
                     /gene="ybtS"
                     /locus_tag="ACHKAY_001750"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO5700441.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(29192..30107)
                     /locus_tag="ACHKAY_001751"
                     /pseudo
     CDS             complement(29192..30107)
                     /locus_tag="ACHKAY_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(30269..30344)
                     /locus_tag="ACHKAY_001752"
     tRNA            complement(30269..30344)
                     /locus_tag="ACHKAY_001752"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30309..30311),aa:Asn,seq:gtt)
     gene            complement(30445..31242)
                     /gene="mtfA"
                     /locus_tag="ACHKAY_001753"
     CDS             complement(30445..31242)
                     /gene="mtfA"
                     /locus_tag="ACHKAY_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO5700442.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            31336..31425
                     /locus_tag="ACHKAY_001754"
     tRNA            31336..31425
                     /locus_tag="ACHKAY_001754"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31370..31372,aa:Ser,seq:cga)
     gene            complement(32093..32743)
                     /gene="zinT"
                     /locus_tag="ACHKAY_001755"
     CDS             complement(32093..32743)
                     /gene="zinT"
                     /locus_tag="ACHKAY_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO5700443.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(33000..33635)
                     /gene="msrQ"
                     /locus_tag="ACHKAY_001756"
     CDS             complement(33000..33635)
                     /gene="msrQ"
                     /locus_tag="ACHKAY_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO5700444.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(33636..34640)
                     /gene="msrP"
                     /locus_tag="ACHKAY_001757"
     CDS             complement(33636..34640)
                     /gene="msrP"
                     /locus_tag="ACHKAY_001757"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO5700445.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(34749..35162)
                     /gene="hiuH"
                     /locus_tag="ACHKAY_001758"
     CDS             complement(34749..35162)
                     /gene="hiuH"
                     /locus_tag="ACHKAY_001758"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO5700446.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            35295..35966
                     /gene="hprR"
                     /locus_tag="ACHKAY_001759"
     CDS             35295..35966
                     /gene="hprR"
                     /locus_tag="ACHKAY_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO5700447.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            35966..37324
                     /gene="hprS"
                     /locus_tag="ACHKAY_001760"
     CDS             35966..37324
                     /gene="hprS"
                     /locus_tag="ACHKAY_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO5700448.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(37432..>38159)
                     /gene="hchA"
                     /locus_tag="ACHKAY_001761"
     CDS             complement(37432..>38159)
                     /gene="hchA"
                     /locus_tag="ACHKAY_001761"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO5700449.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT         8388 a        10138 c        11001 g         8632 t
ORIGIN      
        1 ttattttgat atgattattt ctttagtata aggagagaaa ggaacgagaa agttaaatct
       61 atttatccta atcgcaattt cattttttaa ataatcacta tggattaata ttttttgcga
      121 ctctgctgag ttactggttt taactagtgg tgtaaaaggg ataccttttc ggtatccctt
      181 ttacaataat cagaagaaat caattcgcgt attgataccg acggtgcgac ccatattgac
      241 ctgcgcgacg gcgctgctgc cgttcatgta gccataggta cggtaacgac ggtcgaacag
      301 gttatcgaca tagacggaaa tgttcatccg ttcagtcgcc tgccagccca ggctgctgtc
      361 cagggtggca taggtgcctt gccgcaactg gttgtcgcca tcgaaataat gcggcccgac
      421 cagattaacc gccagtcggg gcatcagtgc gccatagcgc gtatcaatca cgccgttcac
      481 gctgcttccc gcgccataac gtggtacgaa cggcacccgg ttaccgtgat acaactcact
      541 gtcattggtg aattcggaac ggatcacgtt gccattgata tcccatgacc agcctggcgc
      601 aaaccgccac ttcgcttcaa gctcaacgcc ggtggcgtcg gctttacccg cattgcttaa
      661 tgtctgcatc ccgtccgggc cagagtaaag ctgcatgtct ttggtgtggg tataaaacgt
      721 cgcggcttgc agcgtgacgt cagcggtttc gtagcgggtg ccaagttcat agttgatgga
      781 tttctcggcg acgaacggtt tggcatcaag acccgcagta ggcacgatgt tgtacccgga
      841 aggtttatat ccctgggcta cacgggtata cactctccag tcatcggtca gcatatagcc
      901 tgcggatagc tgcccgagca cctgatcgtc attgctctta ccctggtcgc caaacgggtt
      961 gccgagcatg ctgccgtgat attgtgtact ggatttatca tgcgagaagc gcacgccgcc
     1021 gccgatatca aaacgatcgg ttaaatgcca ggtcaggtca ctgtatgcgg ccagcgtttc
     1081 agcggtggta tagccggtac tgcttaaata aggcattgtc ggcatgtcgt aggctgaatt
     1141 taacttctcg cgggtgttct gccggtacag cccaaacacc atatcaacgg tacgcgcatc
     1201 gcccagggtt gcggcgcgca gctcctgcac atcctgattc cagcgctgag gcatattgac
     1261 gattaacgaa ccggaaggga aggtgcgcga ataatgctgc tgctgccagg cgctgatcag
     1321 gttgaaaacc cagtcatcgg tggtgtattt cccactcagg gtttggctgt cagtgcagcg
     1381 ccgcatgtac gggtctggtg aaccatcgct gatcgacagc ttacggccct taatatcatt
     1441 ccatcccaca taggcatcct gggtggcgcg agtacattcg cgtgaggcgg caaagcccat
     1501 ttcccagggc tgatcgtccg gcgccagacg cagtttcaca ttccctatgc tggcgcgggt
     1561 gccgcctaag tcatcgcttc ccgtcgcggg gttaatcatg tcgccgtcat caacctggcg
     1621 taacagggtg acgctgccgt acagcaggcc atcctcaatg gggccgctca ggttgaactt
     1681 acttcgataa ctgtcgcggc tactgacgcc gccttcaata tagccgcgcg gcgtgctgtc
     1741 cggctgctgg gtgacgatgt tgatgatccc gccctgagcg cttttgccat ataacgttcc
     1801 ctgtgggcct cgcagcaact ccacgctttg cacatcggta agcgcctgga tggtgttggt
     1861 ggaaagctga gggacgccat cgacatacag ggtgacggcg gggttataga agtcctgcgc
     1921 tgaagagacg ccgcgtagcg agatcgtcga aaaaagcatg ttgccgctat tttcaatatt
     1981 gagcccgggc aagactctgg ggagtttgtc gctggcggtg acgccggcgt cgcttaattc
     2041 cggcgcgctg acaacagtag acgagacgtt gttggctgat gccgagcggg aagattgttt
     2101 actggcggta accaccagcg tgctttcgtc ttgctgtgaa gtctgggcat tagcaatggc
     2161 ggggagcaat aatcccccca aggccagagg ataaagccgt gtcattttca ttgttgtgag
     2221 tccctgtaag acggcgaaac gccaaaaata ccgacaatgg tgaattgaaa atgataacta
     2281 tttctaatag tggtgctacc cgaaacgggt ttatttcaac ctgtttcggg tcggtttgcg
     2341 cttattgggc agaatggcga taacggtcga cggcgagagc cgtcaggcgt tttttgtcta
     2401 tcttgccgac ggcggtgagc ggccagtggt ccagaaactc gatttggtca ggaattttcc
     2461 acgcgctgag ccccatacgg gtcagttgtt gacgcaactg ctgatagtca gttggcacct
     2521 gctgcgcgat gataaacgcg caaatccgct cgccaagcag cgtgtccggc gcggcgacca
     2581 ccgcgcaatc ttgcacttcc gctaaacgca gcagtgccga ttccacttca gccgcggcta
     2641 ttttttctcc ggcgcggttg atctgctctt ttatgcgtcc ctcaacgtgc aggttcccca
     2701 cctcatccag cctgacattg tcgcctgtgc ggtaaaaccc ttgcgcggta aaggcctgcg
     2761 cgttgtgggc aggggcgcgg taatagcccg aaatggtata agggccgcgc gttaacaatt
     2821 gcccggtttc gcccggcgcg acgtcgttct cgtcttgatc aacgatgcag atttcatcca
     2881 gaggggacaa cgggcgcccc tggctgtgga gaatggtggc atgcggatcg tccagccggg
     2941 taaaacagag caggccttcc gccataccga aaacctgttg cagggtacag tcaaaggtgg
     3001 cgataacctg ctcagcaagc gtcgggtcga gccgagcgcc gcctgcctga atgacgcgca
     3061 gcgacgaaag gtcgctgtct tcccactccc tggcctgcac ccataattgc gccagcgccg
     3121 gaaccagggc gacgtgagtc actctttcct gcgcgattaa aggcatcacc tcatcacagc
     3181 tggcgctgtc ggtcagcacc acttttccgc cgcaggcaag cgttcccaga ataccggggc
     3241 aggccagcgg aaagttatgc gccaccggga ggacggcgag atacacgctc tgttggctga
     3301 tgccgcacag ttcagcagaa gcgctgaagt tatagctata gtcggcatgt cggcgcggga
     3361 tgagtttggg cgtgccggtt gtgccgcctg agagcaacaa caacgcggtg gtggaaacat
     3421 caggctgtcg ctcaccgtga agggagaaga gcggcgtaaa atcgtcgctc atggtctctc
     3481 cagcgaccag aacatgacgc aagcaggcgt gtttgtgtgc catctgtcgg gccagctctg
     3541 cgtggttttc cccgtgaata acgtaagcga cgggttgcgc cagctcaatc agcgcgtcga
     3601 tatccagcgc ccgttgcgag ggcatcgcca gcacggggat aacgcccagt cttaacaagg
     3661 cgaacagcag ggtaacaaac gcgttgtcgt tgggaagctg cacaattaca tgctcccccg
     3721 aacgcaaacc cagtgatgac agacctgcgg caagttcgtc aacttgtgca tcaagcgcgc
     3781 tgtaggttaa cgaccccttt gcatcaacga cggcaattcg cgaggcataa cgggccgccc
     3841 agtggcgcaa ctgttcggca atgggtaagg gatactgttc aatcagagat tcaaaggaag
     3901 aattcatgcg tcacctttct gaagtactgg gctgtttttg ccacgcggtc attgcagaaa
     3961 atgcgtgggg aaaatggcgg acaatctgcg taaaaaagga ccgggcttgt tgaatgggat
     4021 agaagtgatc gccgtcaatc accaccgggc ctgtaacgtg gctcagccac tgacgccagg
     4081 catcgacctg ctgccaggag gcttcgcgat cgtggctgcc gcacaataac agcgcaggcg
     4141 tgcgcagcgg cggacagacg tcgggcgagt cgtagtgata gctctcggtg gcgtaaaaat
     4201 cagcgcgcag aagaggaaga aacagcgaca ttaattcctg gttttcccgc agttcaggag
     4261 aacatccgcc aatgtctatc agctcagcga taaagtcggc atcatcgcga tggctgagct
     4321 ggcgttcaga atgcagatgc ggggcatggc acccggaaat aatcagtcct tgtggcgcaa
     4381 gcccccgttg ctccagaagt cggcaggttt caaacgccac ctgcgccccc atgctgtgcc
     4441 cggcgagtaa aagcggcgtg tcaggcgata cggatgcttc cagctcgttc gccagcagtg
     4501 ccgccagttg tgtaatgctt ctgagcggtt ccagatggcg aaggcgatcg cgccccggcc
     4561 aggtcaccag agaaagcgcg caatcagcca gttgctcagc ttgccagtgg cgaaacgcgc
     4621 tactgctgcc gccagcgaaa gggcacatga ccagatgcgc agtattgccg ttccgggcgg
     4681 gccacagcgg gatgcacatt gcagattgcg tcacagcgcc tccttatcat catcgttgta
     4741 gaaaccggct aaccggtcgt ggtgcggcgg cgtcagacgt ctgatctccg cattgccggt
     4801 cttacgtaaa atctgctgcc ataaacgtga taacgcgatc tgatgtacgc tttggcaggc
     4861 ctctggtgga tgttcgcccg ccagattgct gcgcaactgg tgtagcaacc agctgacgcc
     4921 ctccggcccg acggtttcgc agcagtcgcg ccagctcagg ggcgccgcgc tgcgcgtcag
     4981 acccggcaga tcgcgcagga tctccggtct gcgccagaga ctgtgggggt tctgctgatg
     5041 gtctgcgaca tacagactgg atgaccagat gacggggccg tagctcgcct ccagcgaaag
     5101 atgaccttcc ggccagccca gcagcaggcg gtgcatgata aggctatgca tatctggatc
     5161 gtcaggatcg agatagcgct gaagcagcag gcaggcttct ccctccggcc agaagagtct
     5221 cagacagtga aaatcgctaa agctgccaac catgtcgcac tccacggcgg cggcatcaac
     5281 gcccagagcc agcaacaaca aatccagagt ggagtagagc aactgtcggc tggtggtggc
     5341 gtggactacc ggcggcgtct tagccaggca gcgacgcagc tgctgcgcat cgcgaagcca
     5401 tgtgcgtccc gcgcgcgtat gcggataaaa cgtgtttacc caatagcagc agccctgttc
     5461 ctgcgccagc gtttgtaagg aactgatgtc atccgggtgg aggggatgct cctgaatgac
     5521 gtgtacgccg cgcgtcagaa agtgtctggc aagctgcgta ccggttcctc cggcgacggt
     5581 cgaacgcacg acaatacagg cgatatccgg catcctggtt atctgttccg gcgaggtata
     5641 cagcggaatg ccaaacgcat gagccagctc tcttgaacgg gcgcttccct gcgccagcag
     5701 gccgaccagt tccagcccct ccgggggctg cataaaggca ttcaggtaca tttcgccaaa
     5761 tttggcgccc acaatcagta cgcgttgttt tggggaggcg gacggcatca taacgtgttc
     5821 tccggttgcg ttgaggttgc gcaaagccag cgcgtaatgt gttgcgcaca agcctgaacg
     5881 tggggggctt ccatcatgat ttgccagtgg ctggcttcaa tcacagcgtc gtcggcgttg
     5941 tttatccagc cctgcaactc ggtttctgct ggcgtccagc gcgcgggtct cccggcggca
     6001 tacaccatga gacaggggac cggaacgctt tcgcccggcg tatgctcagt cagtaaacgc
     6061 agcagatgcg cgatgttgtc gagccacgtt tctgccgctt gcagcgtcag gttttgcgac
     6121 accataccgg ccgttttagc gaggctgata aagtcggcaa gctggctgtc gggcgtctgc
     6181 tggtcgaaat gttcgggcag tgccagaggc gtttctcctt cggctaacag gcgcagcagg
     6241 gccgcccggt tttcgcaaca gaaatcctgt cggcacacgg gatcgattaa caccatccga
     6301 acctgcttgc ctttggcgta caggcgctgt gcggcgcccg ccgcaagaaa cgcgccatac
     6361 gaccaacccg ccagcacata agggccgtga ggctgctgac gacggatgca cccgacatac
     6421 tcatcgatca tctggtcgag cgtagcaaag cgctgcggcg attttgcttg caggccgaaa
     6481 acgcgcctgt tcaacgcgct agcgagcggc agccaggcgc tgatatcgcc gtctgaagcg
     6541 tggaagacaa acagggtttc ctcaccgtcg ccctggcaaa ggggtatcgg gttgtcctct
     6601 cctgacgtag ccgcctgtag atgggcgcag aagtcgctca gcgtcgaatg gttgaacaga
     6661 tcctgaaggt tagccctggc aatccctctc cggtttagct gcgcgaccat ccttgtcgcc
     6721 atcaggctgt cgccgcccag ttcgaaaaag tcgtgatgcc ttgccacggg gcgagacagc
     6781 agcgattgcc agagttcagc cacctgtttt tccagcgccg aattcccccc ggcaactggc
     6841 gtgggtttgg ctggctcagt cggcgcatca gacggggcat gcagcggcgt aaataacgct
     6901 tctctttgcc ggatctgtaa aatgggcaga ccggtgccaa agcgctgctg taaatagcgg
     6961 ctcacggctt ttttatccgg gcgatttacg ccaggcgaac gcgctaccag cagatgctgg
     7021 cgcagcggcg acgattcctg cgcgggccac gccagctcgt ttgcaaaccc ggcctgaacg
     7081 agaacctcct gccatgcgga gcgggtgagc atcggtttct catcccggcg gcggaaatcg
     7141 cggtatccgc ttaatccctc aataaagccc acgctcgcca gctggaatac gctgtttcgc
     7201 tccgtcgctt caacgatcag caaacgtccg ccggctttca gcaacagttt taatctgcgc
     7261 aacgtctgga cgacatggct ggcgtcgtgg agcacattga cggcaacgat caggtcgtaa
     7321 cccgcctccg ggtgggcggt gaaatccagc ggctggttga tgtcgaacaa ggcataagac
     7381 acctgcgatt catcatggaa acgggctctg gcgtcattga ggaactgcgc ggagacgtcc
     7441 gtgaagtggt acgactgccg cgtggccttc agcaccggcg cggtagtggc tgcggttccg
     7501 gcgccaacct ccagaatccg ttctgcgctg cacaaggcgg caatctgcgc ggtatagcga
     7561 ttcagacagg cgctggcggg gttgtcgcga tacagcgcgt cggtaacgcg atgctgctcg
     7621 ttgaacagca attccagcgg agaacactgc ccggagaaga gggcgtcgtg ccgggcgatg
     7681 caggtttcca gatactgcgc cagcgcctgg ctccattggc tttgcgggca cgcttcctga
     7741 ggctcaggaa tctcgctgag cggaatgcgg cagcgccagc tttcaccttc gcggattaac
     7801 cactctcttt ccgtcagaca ctgtagccac tggcgcagca gacgctggtg agacgcttgc
     7861 gcggacagcg cctgtactat tgcgctaaag cgatgggcga tctccggcgt ggtaaacagg
     7921 ccgtgacgat ttagcgtagc cgcgatcccc tgtagcgcac gcgcctccag ccagcgccag
     7981 gtcgcctcca gctcatcaag ctgcgcctgc tcaggcaacg gcagcgccag ttgggtaagt
     8041 tcgtgctcat caggcatcgc cgctgatggc gaaggatcgt cggccatcac gatatccagc
     8101 gtcaacgcgc cctgttcggt tatttccgct ctggcctgcc ggatgccggg gtattcgagc
     8161 gtcgcctgct caatatcccg caggtcgtag tccgcctgcg cgttcagcgg ccagcgtcgg
     8221 cggggaatgt gcccggcgat gccgctggcg agagtcttca ggctttcagg ggcggcgatg
     8281 acggcttgca ggatggcgca atagtcatta aacatcgcct cggcagcgcc gggttccagc
     8341 acgttgtcca tgcagtacca gctaaacatc aactcgccgt cgctctccat gacctgatga
     8401 tccagccaga cctgcggcgt ttgcgtgaat acatagcagg gttcgccgaa cagatggctc
     8461 atcgcctgat cgatagtcat gccttccagc gtcatcccca gcatactggt aaacactacc
     8521 ggcatcagcg gttgacgttg tgatccgcgc aggcggccca gctcacggat cacctcaaca
     8581 ccgttcattt cactgtgcgc catattttgc cagaggcgct gttgggtctg ttgcatctgc
     8641 tcttgcaacg tcactggcgc tgagaagtta aaatcaacca gcgtgacgga ggtaaaatca
     8701 ccaatcaact ggttgatttg cggatggatc ggctggcgat tgaagaacgt caggttcagc
     8761 gtaaatgttg tggtacggct ccagcgctca agggtggcgg caaacagcgt gagcagcgcg
     8821 gcagacggtg tgacgccttg ctgctgccag cgctgtttca cggcctgcca ttctgtcttg
     8881 ccgatcgtcg atttgaaggt ggtgaagtgt ggcgtttccg ggggcgtctc aaccatcggc
     8941 agctctggcg ctaagggcag ttgcggcagt ttttcctgcc agtaatccca ggcatcgtgc
     9001 catgccgatg tctttcggcg cgcctgttca gccatcacat aatcacggaa ggtaatagcg
     9061 agcggcgcca gcgtttcacc gcgccagacc tgcgccaggt cgtccatcat gactttaaaa
     9121 ctctgcacat caaactgcaa aaggtcgagg ttcatatgca gacggtaatg gcaatcgtcg
     9181 atttcgctga ccaccagctc aaaaagaggc cactggtcgg caggcaaaac gcgatagctc
     9241 agttcatgcc gccgtttttc cagcgcgatg cgctgttctt ccggggaaag cgcgcgcaga
     9301 tcgtcacgcg ggatgtgata ctccggcgtt gtcgccagga ttcgctgctg cccgtcggca
     9361 tcaaccacca tacgcaacat atcgtggcgt gcgatgagct ggttccatgc tttctccagt
     9421 atggctagat cgaactcatc gtggcgttta tcccactcaa acaggacgtg acaggcgacg
     9481 ccgccatagc caatgaggtg ggttcgcccc agccagtagg cgtgctgaat gggcgtcaaa
     9541 gggaagggcg cataacgctc gtcggcgtcg tgccgcaaca cttccggctg cgaaacggca
     9601 ggcgtcgcct ctggcttaga acagataagc tgcgtgagtc catgaggaga cagatcctgc
     9661 cacgcccgtt ccgcattgat gcgtacgccc aggtagtgct gaatatcgct actgagttca
     9721 aggaagagca gcgagtccat gccgagttgc aacagatcct ggtttggatg cagtgacgcc
     9781 ggatcgctca gccttagctg aaccgcaatc cgctttttca gccatgccat caccgctgtt
     9841 tcatcgctca ggctgccgtt aaaggcgttg tcatcagcag gaggaatgac cggcgttgcg
     9901 gctttttctg tggcgctgat gttaaacaga gcctgttgta acgggggcat tttgtcggta
     9961 aaaacgcgca tcgccagtcg ccacggggcg ccgcgcatca ccgcctgttc caggtgccag
    10021 cagccttcgg catcgcttaa cgcgcccata cctcggctgg cgagcgtcgc cagcatttcc
    10081 ggcgtggccg cccgaccgct ttctccccat gcgccccagg cgacagagag cgttttcggc
    10141 gcatcaaggg tggaaaactg ctgggccagc ccgtccaggt agccgcagga cagcgcatgg
    10201 gcgctctgac ccggcgcgcc gagggtggcg gcagcggaag agtagagaat aagatagcgt
    10261 ccgtcgtggt tgcgcagggt ttgcaacagc tggcttgccg cctgcgcttt taccgcgaaa
    10321 acggcagcca gctggtggtc atcaagctcc tgcaagggcg cgtcagccaa tacgccagcg
    10381 gcatgaatcg ctccggcaat gccgccgttg gccgccagat cgtcaagaac cgtggccagt
    10441 tgcccggcat cgcccacatc acaacggcag acgcgcgtct gcccgccctc cacgtcgcgt
    10501 agccatgact catccacgtg cgatgccagc agggcgatgc gtcgcgcccc tttttctctg
    10561 agccagttca cggcaaggcg gcctaatccg ccaaacgctc cggtcaccag atgccagcgg
    10621 ctatcgccgg taaacacgtt tgccggtaat tcagctgcgc atcccgtatt gggcgccagt
    10681 gaagggagcc aaagggtgtc accccgtgcg gcgagccagc gctgtgatag tgagactgcg
    10741 ctcaaccctt gatgcagcgt ttcccacggg gtgttttcgg cgagatcgat ggcggcaagc
    10801 aaccgttccg gctgttcgtt ggccgcgacg cgaagcaagg cccatagcgc gtgatgggat
    10861 gcagagagtg cttcattttc ctcgactcgc cacgcgcggc gagtaaccac aatcaatccg
    10921 gctgcgctgg cggtgagcgc tgctatcact ttctccgcta aggccagcgt atcctcgctc
    10981 tcctcaacaa tcatcagtcg tgaagaggcg tgcggatcgt gaatgatgcc gtattgcgcc
    11041 agctcgtcgc tgcgcgcaag cgtaccggcg ctgaagctaa aggtaagcgg atggtcaatg
    11101 ctggcgacgt tgaggggcgt ccagcgccat tgataatgcg tttgcggcgc aggaagaggc
    11161 gcggcgggcg atagcggcag ccactcgcca gcaggatgac gggcttcaac gcgcatctga
    11221 ccgcgggaaa aggcttcgtt ttgccagctt aagcgaacct tccccagcca ctccggggcg
    11281 gcgacggggg gcggttccac aggcagagcg tcgccatgac gctgtgaaag cagacgccag
    11341 gcctcctgcc agcgggcgtt aaactgttcg ggctgacctg cgcaatcaga ccagtcggcg
    11401 agataatcac cgttatccgt cagcgcctgc cccaacacgg gttctgatgg cgcggtgaat
    11461 gttacggcgc taaccgcctg accgggcagc gtggcgagaa tgatatgttc gctcatcccg
    11521 gcggtcgggc tgccatcctg cggtagccac gccactttgc tgaatccggc gtggcggcac
    11581 tgttgttgcc actgagcggt ggtgaggaat aactcacctt cgcgggcgtc gagatcctgt
    11641 agcggaagaa ccagcgggcc gaaaacgaag tcaaacagac gcattggctg ggtgatttcg
    11701 cgcatcagca ggcgcccgcc cggcttgagc agggggcgca gattatcgag cgtgcggcca
    11761 atatggcggg tggcgtgaat cacgttcgct gccacgataa gatcgtaaga ctgtgcctgg
    11821 aaaccctgag actgcgcctc tttttcgaga tccagctcgc tatacttcac aaaatcatag
    11881 tcggcgaatt tctgctgggc gcgacgggtg aacagcgccg agatatcggt gaaatggtac
    11941 tccagtgccg gaacgccgtt gagttccggc agcagccacg cggtggtgcc gccggttccg
    12001 ccgccaactt caagaatacg caacgactgg cggggctgac gcgtctggac aatgccgcgt
    12061 aataccccgg cggcgatttg gttgaaatag cggccaaagc tgaattcctg atacagcact
    12121 tccacgccgt cggaggcgct ttgcgggaag ataatcgcca ccggttcttc cgcgccgctc
    12181 atcatttcat ataaccgatc gccggcacgg gcgatggtgt cgggaatagc ctgaaaacct
    12241 tcacaataac tggcaagttc cgtcagcagt gattcccgct gttgatgttc aatggggcgg
    12301 gcgcggacgt atcgcccgtc ggtgcagcgg taatcgccgt cgaccacgca gttattcagc
    12361 aggcgctgga gtagctgctg gtagcggggc agcagacgtc cacggcgcat gatggtcatg
    12421 gcgtccacgc cgttctcaat ggcatcgccg gtacagcgtt gtaccagttg atcgacgtag
    12481 atggcgtgca gtcgcgtggc gcactgttta agcgcttcca ggcgagggag atcgagcgct
    12541 gtcgcggcgc gactcgccac ctccagcccg gcagacagcg ctgcgtcggc aggctcgcag
    12601 gccggggaga cgcgctcttt ccagtaacgt tcagtatcaa acggataaca tggcgcagcg
    12661 atacgttgtc catcgcccgc cagcaggtcg gcccacggta gggcgacgcc ggcagcgtaa
    12721 agctggagca gggcctgatt gaggacatcg ctcgcctctt tgttacgccg ggcgctggct
    12781 atccagtatg cgttatcgcg gtattcgcgc tgcccgcaag caaccaactg ggcatcgggc
    12841 cccatctcca gaaaaacgcg ggcgccgagc tgatgcgcca cctgaatact ctggataaaa
    12901 cgcaccggct ggcgcatgtg tcggcgccag taatccgcct ggttgagcgt tgactcatca
    12961 atgacgtcgg cggtgagcgt ggaaataatc ggtatttgcc ccggctccgc gtgcagtccc
    13021 gcgcaggcgt cctggaaccg atcgagtatc ggctccagta aagcggagtg cgccgcaccg
    13081 gttacgctca ggcgacgata gttaatgtca tgctgcgaga gcgtggcgca aaataccgcg
    13141 agacgggctt ccggcccgga aaataccgta tgttgcgtac cgttgttggc ggcgagatcc
    13201 agctcaaact ggcgagccag cggcatcagc gtgtcttcgt ctgcaaatac cgccaccatc
    13261 gcgccgcttg cgcactgctg cattagcgcg ccgcgccgac aaaccagtgg catgacctgt
    13321 tcaatcgtat agtgtccgca gacaacggca gcggcaaatt caccgacgga atgcccaatg
    13381 gcgaagtctg gcttcagtcc ttcagcacgc cagtgcgccg ccatcgcgat ttcaaacgcg
    13441 acaatcgccg gctgcgccca ggccatattg tccagctgcg ccgaatcggg gttaaacatc
    13501 gcttcgcgca gtgatggcgt gagcatttcg ctacaggcgg aaaaacagcg atccagcgtg
    13561 tcggcaaacg ccgttgagtg ctggtacatc gtttggccca tagtgtgcca gtgcgagccc
    13621 tggccggtaa acagccacac ctgcttgccg ctggcgccgt ggccgctgta aaccagcgcc
    13681 cccgatttct caccggccca ggcgctgagc gcctcggcgg tttcacggtt taatggcgcc
    13741 gccaggcgga aggggagatc gagacggcgc gcgtgcaggg ctgtgaaggc cagagagctg
    13801 gcatccgcat tctctctcag cgccccggca taatccgtcg ccagccgccg caacgcgctg
    13861 tcgctggcgg cgctgagcag cagcgcggta ctttttctgc cgctatccgt attggggagg
    13921 cgcgcgttga gcgcgtcggg cagcgaggcg acgatcatat ggcagttggt gccgccaata
    13981 ccaaaggagg agacgcccgc atagcgcatt tcgtcctgcc atgcctgtgc cgacaccggt
    14041 atggtaaagg ggctctcttc aagtttcagc gccgggttgg gggtgtgaaa attcagtaag
    14101 ggaggaattt gcccgcgact gactgccaga acggttttca gcagtccggc aatgcccgcc
    14161 gcggtatcca gatggcccat gttacttttc acggaaccga gcgcacagcg ctgatcctgc
    14221 gggcgaggcg catagacgtt gcgtaacgct tcaatttcaa tcgcgtcgcc cagcggtgtg
    14281 ccggtgccgt gggtttcaat gtaacctacc tgcctgtcgt cgatggccgc cagcattaac
    14341 gcctcttcga tgactgcctg ttgccctgcg acggaagggg cggtatagcc gacctttctg
    14401 ttgccgtcgt tgttgaccgc gctggagagg atcaccgaga taatcggatc gcctgacagc
    14461 agcgcgtctc tcaggcgacg cagcaccacg cagccgagac cgttaccggc ccaggtgccc
    14521 tcagccgagg cgtcaaaggg acgacagtga ccatcaggag agaaaatcat tccgggctgg
    14581 tagcggtagc ctgcctgctg ggggaaagag agcgccacgc cgccggcaac cgccatatcg
    14641 gattcgccag cgcgcaggct ttcacaggcc agatgcacgg caaccagcga gctggagcag
    14701 gcggtctgta ccgataacgc cgggccgtgc aggttgagtt tgtacgcggc gcgggtggca
    14761 atatagtctt tatcattgcc catcagagat tgcagacctt ttacctgcgc gacttctgtc
    14821 acgttcaatg cttcgcgacc ggggtaggta ctcatccggg aagaggcgaa aacgccggtc
    14881 ttatggggga cggcgccggg ggcataaccg gcatgttcca gcgcatgcca gaccgcctgc
    14941 aaaaacaggc gctgctgcgg gtccatcgac tccgcttcct gtcgcgaata gccaaacagg
    15001 gtggcgtcga agcagtcggc gttgtctaac accgaaccga tattgacata atgaggatcg
    15061 tcaatgatgg cggcatccag accgacggcc agaagctctt cgcgcgtaaa gcgtcggctg
    15121 cattcacgac cttccagcag attttgccag aacgtttcgc catccggcga ttcaggaaaa
    15181 tggcaggcgt agccgataac cgcgacaggt tcgcagtccg ggtagtgttg agcgatcgat
    15241 gcatcaatgg aatctgctgt cggcgcagaa gagaagcgca agttatccat aatcggtccc
    15301 gtttcctaag attcacaaag tcaatggaac gccatgcgtt cgcgatggcg ttccggggaa
    15361 aatcagtttg cttcgcgcta tatccgccgc tgacgacggc gaacagggcg aggtcgttgt
    15421 gttttctcca ccgtggcgac gccggccaga taatcggcca gggcagcagg agaagggtgg
    15481 gtgaagaggt caagcaacgt cagggtggaa aattcgtgac gttgtaacaa aacgtgcagt
    15541 tgcaccagat tcagcgacgt tgcgcccgcc tcaaagaagt tttctgcggg cgtgacagac
    15601 tcgccaacca cctcgcggaa ggcgtcgcaa atcaggctga cggtgctctc gtccgccgca
    15661 atgtgtggct gagagtgctc agggcatacg cgcggtgagc gctctgcgca ttgccctgcg
    15721 tgtttaacgg gaggcaccag cgcggcgagc ggcagttgcc agccgctttc atcctcggcc
    15781 attcggttta gcaatgcgca atactgttca aactggcgct ccacctgccc ggcaggaaac
    15841 agcgcggcga caaaatccca gttaaagcgc agttcgcctt cggattcata aatctggtga
    15901 tcgagccaga cctgcggcgt ctgggagatg ccccagaccg gtttgagcag attacgccgg
    15961 gcgaggaagt tatcctgctc aaagcccagc gcgctggtaa agacgacggg catcggaacg
    16021 gctggcacgt tttgccgttg cgccagttga cgcatcacgc ggatggctga cacatcgcgg
    16081 tggttgaggt tctggctcag ccgctgctgt agtgactgcg cgctgtgcag ccagctttcg
    16141 ccgggatgcc agctcagcag catcagcgag gtgaaatcgc ccagaatctg gttgatttgc
    16201 gggtgcagcg gtcgcctgtc gaaaagcgta aggttaagcg tgaagtcagg ctgtgcactc
    16261 catgcagaga gaaccgttga ccacaccgac aacagtacgg ccgacggggt gagatgggcg
    16321 tcagccgccc gttttttcag ccgatgccag cgcgtgctgt ccagtgcgcc gttcaggcgg
    16381 gcgaagcgcg gtgtttcaac ctcctgaggc aagcagcgca gcggcaacgc aggcgccgga
    16441 ggaatatcat caagctgcgc ctgccaccat gccagagaat ctggattggg cgactgtagc
    16501 gagggttgtt gcagataatc cctgaaggtg acgggcagcg gcggaagcag ctgttgcggg
    16561 tagcggtagc cgtgctccag ctccgccagc aggatctgca tgctcagacc gtcaagcaac
    16621 aggttatcca gacacagcca caggcgggca ggcatcccgt ccacgtatcc gacctggaga
    16681 tcgaataccg gccatacttc ggggttgagt acctgatgcg ccagtttttc gcgcacccgc
    16741 aacgcctctt caggcgtatg gagggtgtgt gcgggtatca cccagggggg cgtctgttcg
    16801 agcacctgtt gctgtccatc acgcacgatg gcgcgcagca tatcgtggcg ggcgattaat
    16861 cggttccaga ccgtctccag ccgggtgagg tccagatcgg caatttcaaa ttcaacaaag
    16921 aaatgtgagc cgacgccgcc cagggcaaag cccggctgac gccccaccag gtaagcctgc
    16981 tgcacgtcgg taagcgcaaa gggctggtag cgatcttcag gggagtggac gaatggttgt
    17041 tcgaccggga cgtcggtttt ccgcagcgtg gcggcaaaat ccgccagccg gggatgatta
    17101 aacaggtcgc ttaattgcgc ttcataacct gcctgatgaa gttgcccggt cagacgggtc
    17161 gccagcaggc tatcgccgcc ttgctggaag aagtcggttt ctctggtgac attgccagtt
    17221 gataagagtt gctgccagag ggcggcaacc tgtttttcaa tgtcgccctg gggtaaatcc
    17281 gcttcggccg ggttttccgc ttcaggggta tgacgacgct tcagcgcctg gtagtcaatt
    17341 ttcccgttag ctgtcagcgg taaggcgtcg aggaagacga tccgttgcgg caccatccac
    17401 cccggcagac gcccggcgag ggcggcctga agttgacggg gatcgcggcg cacctgcctg
    17461 tcaggacatt gtacgaggaa gcgttgtaac cccgcgacct catcgccatg ctgacagttg
    17521 aagccggccg cgctaaataa tgccgcccag tcggcgctgt tatgcagcaa ggcctccggc
    17581 tgcccattgg ttaacaggag cgtgctgagt agggcggacg gcgttaactg gcgaaactcc
    17641 atcacgtaga gcagcgcgcc gggaacggca agctgttgta atgtcgcaag gagcccggga
    17701 tcttccggca gcagacgatg cagggcgtta ttaagccaga taatgtccgc cgagtgagcg
    17761 tgcgccgcca gcgtgtctgc attccagagg gacagacggg cgccaggcca gggggcgagc
    17821 ctctgccggg cgctcagcag catctcctgg ctctgctcaa gcccgacata ctcaatctgt
    17881 ccggcgttga gctgtgctaa cagcgattct gcggcgcggc cagtgcgggt tcccacctca
    17941 agcaggcgta ccgggcgctg taagcgctgt gcaagctgtt ggcaaattgt cgccagttcc
    18001 tgaatatacg gggcgctggc cggatggtta aaggccaggc tttccggcga ccactgcgcg
    18061 tcgttcagta acgacagttc gttacgggca ccgcgaagaa tttgcgataa cgttaaatca
    18121 tgtccgctga agtgcgggtg ctcatcctcg cgttcttcac cagcgcagac ctgataacct
    18181 tcagcagcgg gcttcagtcg ccgttgtgtc accagaaatg ctaaccagcg ttccaccacg
    18241 gcctgccagc gcggctggat agcgagtgag ttcatcaggg ggagaggatc ggcgccagcg
    18301 gtgtgacccg gcttcagttt tagcaggcga tgctgaagga aatcggctac ctgttctgcg
    18361 gagatctctg gcgagatggc gcaacagggc aacgttcccg caagcgtgtg ccacgcctgc
    18421 ggcagtgccg ggttccgatg atcggtaacg caaaaagcct cgccctgagg aacaacgtat
    18481 gccgccagcg ttttttcttt ttcgccgatc gccagaacgg ttgcttgttt cacccccacc
    18541 agctggctga gcgcgctttc gatttcgccc agctcgatgc gatatcctcc gactttgacc
    18601 tgcttgtcgc gacgaccgag gaactcgatt gtgccatctg gccagtagca gccgagatcg
    18661 ccggtgcgat accagcgctc gtccgggagc gtcaaaaatt gctgctcgct acgcagggga
    18721 tcgttgaaat agccttccgc gaccccaatg ccgccaatcc ataattcacc cggcacccag
    18781 tcagggcagt cccggccctg ttcatccacc acccggtagc gttggttggt tagcggaaaa
    18841 ccgtaaggga tggagcgcca gtaggcgggg acgtcgtgaa tttcgcaggc gttagaccag
    18901 atagacgcct cggtggcgcc gcccatcgcg ataaattgtc cttgtggccg gaaggcccga
    18961 taacgggcgg ggaggtcaag cccgatccag tcgccggaaa gcatcactgc gcgcaggttt
    19021 tccggcgtgg cgtcggcgaa accttcacac caggtcagca gcatatcgaa cagcgccggg
    19081 acgctgttcc agagcgtgac ctgatggcgc tggatcagct cacaccatgc gtgaggatcg
    19141 cgccgttgat tttccatcac catcaccagc gcgccgcccg cgcgcagtac gccaaaaatg
    19201 tcgtaaaccg ataaatcaaa atgtagggcg gagagggcca gcaccctgtc atgcgggcca
    19261 acctgatagc gggtattgat atcgcaacag gtgttaagcg ctccccggtg agaaatgact
    19321 acccctttcg gcgtaccggt agagccggag gtgtagataa tgtaggccgg ttgcgtgggg
    19381 gcgcgtacta ccgggttggc gatcggctcc gcctcaatgg cctgctgcca ggcaagggcg
    19441 ggaatatcgt ctgacccggc gctggcgtcg tgctgacaaa tgagcaccag ccggacgctg
    19501 gcgtcagcgt agattttctc gcgccgtgcg gcaggctgat ccagcgaaac cggaacgtaa
    19561 accgccccgg ccagcaggac ggccagaacc gcaacaagtt gtcctgcgcc tttcgacatc
    19621 gtgatagcca cattatcgcc gggctgaacc ccgcactcga ttaacctgcc cgcgcaacgg
    19681 cgggcatagt ctgtcagctc atgataattc cactgataac gcatgtccgt taccgccagc
    19741 gcctgcggct gttgcagagc gatacggaaa atgccttcat gcagcaagcc ttcgggaatg
    19801 ggggcgccgg tggcgttgac ccgttcgcgt atcgcgcgct ggctggcggg catcatatct
    19861 gcgaacggct tttgccaggc gctttcgtca tcgcagagtt ggttaatcaa ctggcagtag
    19921 gcgtcgaaca atgtttcgac taacgccgga gggaacagcg cgtcgttgct gtcccattgt
    19981 agccagacct cgccgtgatg ctcgaacgcc agatgatcta tccagacctg cggcgtttgc
    20041 gagatgcccc attccggctc gcccaacggc gattctgcgc ggctgctgta gagggaacgc
    20101 cccagattgc tggtaaatac caccggggcg ccgtgggggt agcgctgctg gcgtttgagt
    20161 tcacggagta attcgacgcc ggaccagtgg cgatgctccc agtcctccgt aaacgtgagc
    20221 tggtttttac gcgccaggtt gctgacggta tcgccatcgc aggcggtatc cagcagaaga
    20281 atattggtga agtcggcaag catcgcgcca accgccggtt gcagcggctg gcggtcgaat
    20341 aaggtgatgt taagcagcag acgcgtcagg ccgccccagc gagccagcac ggcagaaaaa
    20401 caggtcgcca gtgccattgt cggcgtcacg ccatactcgc cggcccggtt gctaaaggcg
    20461 tgccagcgtg ttgccgggac aatcatgcgg cgtcgggtat tacggacttc acgtagcgtg
    20521 gcgggttcgc aggccagcgg caagacgggc gcggggggaa gcgtcgatgc tttcgccagc
    20581 cagtaagcgc gcgcgacgtc tctcagtggt tgattgatct tctgctggtg cagcaaatac
    20641 gagcggaaat catagcgggt gtcgatagcc ggcagcgatt ctccggccag cagggcgtta
    20701 agctcatcga agaaaagcgt aaagctggag gcatccataa tcagcaggtc aatattgaca
    20761 tggaggcggt ggcgattgtc cggcaagagc gtcagctgaa aatcaaacgt ttcgccgatt
    20821 tccacgcgta aaagacggtg gctcaggcgc tggcgcagtg cgtccagata ggcctggcgg
    20881 ctttcagcgt cgttatggcg taaatcatga acggtgacgc cgttccagta aggttgcggt
    20941 agccagacct gctgcccgtc ggggcgaaag gcgatatgca gcattgggtg gcgttgcagc
    21001 aaggtcgtga tggcctgctc cagctgcgac gccgtcagac aatggccttc aaactcctga
    21061 tacaggtggc aacccacgcc gccaagcgtc tgccccggca tgcggcccgt caggtaggcg
    21121 tgctgtactg gcgtcaatgg gaagggggta ctttcggtca tgttcggcca ggatgattcg
    21181 tcgggcggcg tttcttcttc cgcgttctcc ggcgaccggc tgagcattaa ctggttccat
    21241 gccgccagcg tgggggcggc atacagctcg cgaagggtaa ggcggtagcc atttttacga
    21301 aaccagtgta accatctcat caatcttatg gaatccagac cggcctggat caggttgctc
    21361 tcttcatgta actgctgcgg cgttaaattc agttcctgta tgagccgctc gcgtaattgt
    21421 tggtaatcag ccgcgtggcg gttgtccggt aacagcgaat cctgagatgg tgcgccagaa
    21481 atcattcttc ctcctgatgg cacgtcttac cgaaaagcat tactcatatg agaataataa
    21541 ttattaacaa ttggatgcta cccgaaacgg gtttatttat gcccgtttcg ggttggtaac
    21601 aggcggaaag aacagaggcc cggatggggt atccgggcct ctgtcaggga ggagtttagg
    21661 ggggcgcgac cccggttaca tcccgcgttt aaaggtcgaa ggagttacgc caaactgttt
    21721 ctggaaggcg gcagagaaat ggctggcgtt ggcatagccc agatcggccg ccaccgtcat
    21781 gacggaggta ttgccgttgg ccaggcgtct tctggcttcc tgcattcgtt cagcctgaaa
    21841 caggccatag acgctgttgt tgaacagaac gcggaagcct cgcttaattt tgagaacgct
    21901 cagaccggac tctctggcca gctccgcgat gcccggcgct tgcgtcagat cagccagcag
    21961 cagatccttc gcccgcagca gtttctggcg ttccatcggg ttgaagtggc aggagaggca
    22021 actggcgtct tcgcgatgtt catccagcac cagactgagc atgaccagac tttgcccctg
    22081 caaccatagc gtcgacggct tttgttcatg attgaagccg tcgtgcatgc gcatcagcgc
    22141 gtgacgcaag gcctgagccg ttaagtgggt ttcagcatta caacgctgct gctggcaaca
    22201 gcagtgggag tcgatcttat tcttaatgac cgcgctaata tccggcaccc acagcgcgag
    22261 ggtttcaggg ctgaaggaga gggtaatgct ctcgaatcgg ccacaatagg aggcgctgcc
    22321 gcgacagtct ggcgtatggg taatgtaatg gcgattgcgc gcaagctccc agtcggtgtg
    22381 gcgccggagt ccctgaatcg caaagcgcga tgtgccttcc aggatgcacg agaagttaat
    22441 cctgcccaga tcgtcgtgta gctgcaacgt ctcaggccgc gcaaaatggc ccctccagag
    22501 caaaatatcc ataccttcct gcaacgaaca gcgcagcagt tcacactgag caggaatatt
    22561 gccatcgttt gcaggtttcc cgaccaccaa ctggtgaata gagatttcag attgcgtttg
    22621 cggtgactcc gtcatgacct ggttatctcc ctgtggctta gaccaatgcg ggtataaata
    22681 gaaccgaatg gggtagatga gttattgata atggttatta ttcacattaa ttctcagaac
    22741 gccaccctga attgtcgttt ttggcgtccg taaaaatgca tcaggaaatt cagttactcc
    22801 catgtcatct caatcatcca atactgaatc gctgtcccga tttcctctct ggcaggtgat
    22861 tacaccggtt cgcaggaagg ttattttagc tatggcgctg gcggggctgg cggcattaac
    22921 cagcctgggg gcgctccttt ttctggtgtg gagcctgcgt gacattcgcg cgacgcctga
    22981 cgctattccg gcctggccgc tgggtggcgt gatcggctgc gtggtcttaa cttttgttct
    23041 gcgcttacag gcgttcaaca cctctcatta cgcggctttt catctggaga acattctgcg
    23101 cagcaggtta gcccgtaaag cattgcagct tccgcctggc gtgttacagc aaatgggcag
    23161 cgggtcagtg gcgaaagtga tgctggatga cgtgaagtcg ttacatattt ttgtggccga
    23221 cagcacgccg ctctatgccc gcgcgatcat catgccgctg gcgacaatcg ttatcttgtt
    23281 ctggctggac tggcggctgg caatcgcgac gctgggggta ctggcgtttg gatcggttgt
    23341 tctcgtgctc gcccgccagc gttcggaaga tatggctcag cgttatcata aggcgcgcga
    23401 gcaggttagc gcggcggtta ttgagttcgt gcaggccatg cccgtggtga gaacgtttga
    23461 tagcggcagc accagttttt tgcgctatca acgcgccctt gaagagtggg tcgatgtgct
    23521 ccaaacctgg tatcgcaaag ccggtttttc agcgcgtttt tccttctcga ttctgaatcc
    23581 tctcccgacc ctgtttgtcc tgatctggtc gggatacggc ctgttgcact atggcagttt
    23641 cgattttatc gcgtgggtgg ccgttttact gctgggcagc ggaatggccg aagccgtaat
    23701 gccaatgatg atgctcaata acctggtcgc gcaaacgcgt ttaagcattc agcgtattta
    23761 tcaggttctc gcgatgccgg agttatcgct gccgcagtct gagcagcagc cgcaagaggc
    23821 gagcattacc tttgagcagg tgagctttca ttatccgcaa gcgcgtactg gcgccgcgtt
    23881 gcaggaggtg agctttcatg tgcctgccgg gcaaattgtg gcgctggtcg ggccaagcgg
    23941 cgccggaaaa agcaccgtgg cgcgcttgct gctgcgttac gccgacccgg acaaaggcca
    24001 tatccgtatt ggcggcgtgg atctgcgtga tatgcagacg gacaccctga tgaagcaact
    24061 ctcgtttgtg tttcaggaca acttcctttt tgtcgacacg atagccaata acattcgtct
    24121 gggcgcgccg gatacgccgc tggaggcggt aatagcggcg gccagagtgg cgcaggccca
    24181 tgattttatt agcgctctgc cagaaggtta caacactcga gtcggggaac gtggggtatt
    24241 tctctccggc ggccagcggc agcgcattac tatcgcccgg gcgcttttgc aggatcgccc
    24301 catcctggtg ctcgatgagg cgacggcgtt tgctgacccg gaaaacgaag cggcgcttat
    24361 caaggcgctc gcggcggcca tgcgtggccg gacggtcatc atggtcgcgc atcgtctctc
    24421 aatggtgact caggccgatg tgatattgct gttttccgac ggacagctca gggaaatggg
    24481 gaaccatacg caactgttgg cgcagggcgg cctgtatcaa cggctctggc aacactatca
    24541 gcaggcgcag cattgggtgc cgggtggaac acaggaagag gtggtggaaa atgaaagaca
    24601 ataatcctgc ggataacctg gcctggcgcg tcatctggcg ccagcttatc tccagcgttg
    24661 gcagtcaggc caggatgctg cgacgcagta tgctggcgct gttgctggcg gcattcatgc
    24721 aggggatcgc ctttgcctgt ctttatccga tcattgatgc gctgttacgg ggagacgcgc
    24781 cgcaacttct taactgggct atggccttca gcgtcgccgc aattgtgacg ctggtgctac
    24841 gctggtatgg cctgggcttt gaataccgtg gtcatctggc gcaggctacc catgagttgc
    24901 gcctgcgact tggcgagcag ttacgccgcg tgccgctgga gaagctccag cgcggcaggg
    24961 cgggtgaaat gaacgccttg ctgctgggca gcgtggatga aaacctcaat tatgttattg
    25021 cgatagccaa tattttgctg ctcaccattg tcacgccgct gacggcgtcg ctggcgacat
    25081 tgtggataga ctggcggctg gggctggtga tgttgctgat cttccctctg ctggtgccgt
    25141 tttattactg gcgccgcccg gcgatgcggc gacaaatgca gacgctgggg gaagcgcacc
    25201 agcgtctgag cggcgatatc gttgaatttg ctcaggggat gatggtattg cgcacctgcg
    25261 gcagcgatgc cgataaaagc cgggcgctgc tggcgcattt caatgcgctg gaaaacttac
    25321 agacccgcac tcaccgtcag ggcgctggcg cgacgatgct gatcgccagc gtcgtggagt
    25381 tgggcctaca ggtggtggtg ttatccggga tcgtctgggt agtgacgggc actctgaacc
    25441 tcgccttttt gattgccgcc gtcgcgatga ttatgcgctt cgcagaaccg atggcgatgt
    25501 ttatcagcta cacctcggtt gtggaactga tcgccagcgc cctgcaacgg attgagcagt
    25561 ttatggcgat agcaccgctt cccgtcgcag agcaaagcga gatgccggaa cgttacgata
    25621 tccgctttga caacgtcagc tatcgctacg aagaaggcga cggccacgcg cttaatcatg
    25681 tttctttgac gttcccggca gccagtatga gcgcgctggt gggtgcctcc ggcgcaggca
    25741 aaactaccgt caccaaactg ttaatgcgct atgccgatcc gcagcaaggg cagatttcta
    25801 ttggcggcgt cgatattcgc cgcctgacgc cggaacagct caatagcctg atttctgttg
    25861 ttttccagga tgtctggctg tttgatgaca cgctgctggc gaatatccgt atcgcgtgcc
    25921 cacaggcgac gcggcaggag gtagaagagg ccgcctgcgc ggcgcagtgc cttgagttta
    25981 tttcccgtct tccgcaaggc tggctgacgc caatgggaga gatgggcggc cagctatcgg
    26041 gcggcgagcg ccagcggatt tccattgcca gagcgttatt gaaaaacgcg ccagtcgtca
    26101 ttctcgatga accgactgcc gcgctggata ttgaaagcga gctggcggtg caaaaagcga
    26161 tcgataacct ggtttacaac cggacggtga ttatcatcgc tcaccgttta tccaccatcg
    26221 ccggggccgg aaacattctg gtgatggaag agggacaggt ggttgagcag ggtactcatg
    26281 cgcaattgct ctcacatcat gggcgttatc aggcgctgtg gcaggcgcaa atggccgcgc
    26341 gcgtgtggcg cgacgacggg gtttccgcgt ctggagagtg ggtgcatgag tgatgttcag
    26401 tcgaatgtga aaccgctgac gttgacgacc gggcgggtga tttttgctat tgccggcgtc
    26461 tatgtgacgc agagtctggt atcggcgctg tctatgcagt ccttacccgc gctggtgcgc
    26521 gctgctggcg gttcgctggc gcttgccggt gcgacaaccc tgtttatgct gccctgggcg
    26581 ctgaagttta tttgggcgcc gtggatcgag cgctggcggc ttccgcccgg tagccaggaa
    26641 cgccgttccc gcatgttaat cctgcgtggt caggtcgcgc tagcggcgat cctgactatt
    26701 gccgcagcga ttggctggtt taggcgagaa gggggattcc ccgatacgca aatcgtcgcg
    26761 ttatttgttc tgtttatggt ggcaggcacg gtcgcctcca ccattgatat cgccagcgac
    26821 ggcttttgtg tcgatcaact gacccgcacg ggttacggct ggggaaacag cgtgcaggtc
    26881 ggcggcagct atctgggaat gatgtgcggc ggcggggtgt tcatgatgct gtcggcggca
    26941 tccggctggc ctgtcgccat gctgatgatg gcggtgctga ttatggcgct gtcactcccg
    27001 ctgtggcgca ttacggagcc gacgcgaaca gcgactatcc tgcatgttcc ggcgttaggt
    27061 tatgcgctaa ggaggaagca ggcgcgcctg ggcttactgc tggtattgat gctgaattca
    27121 ggcatgcggt ttgtgctgcc tcttctggca ccgctgttgt tggatcatgg gttgagcatg
    27181 tccgcattgg gcgcgttgtt cagcggcggc aatattgcag cgggcatagc aggaacgctg
    27241 gctggcggat tgctgatgaa atacacctca cccggcagag cgctgttgac ggcttatggc
    27301 gtccagggga tcgcgctgct ggcggtggtg atgacgctca tgatggcgcc gggtcatctg
    27361 ctgctgcaga ttctccagtg tctggtcatt gtccagtcca tttcgctggc ctgcgcgctg
    27421 gtctgtcttt acgccacgct gatgtcgctt tcatcgcctt tgcaggccgg tgtcgacttc
    27481 accctctttc aatgtactga tgcggcaatc gccattctgg caggcgttat cggtggcgtt
    27541 gttgctcaac attttggcta tgcggcctgc ttcctgtttg ccggggcatt cacgttgctg
    27601 gcggcgtggg ttgcttatat ccggctgcat tcggcaagag aactgatgac aagcgcaatt
    27661 gattgatccg cgaataacac agagaggtaa gggatgaaaa tcagtgaatt tctacatctg
    27721 gcgttaccag aggaacaatg gctaccgacg atttctggcg ttttacgcca gttcgcagaa
    27781 gaagagtgtt atgtctatga gcgtcaaccc tgttggtatt taggcaaagg gtgccaggca
    27841 cggctgcaca ttaatgccga cggaacgcag gcgacattta ttgatgatgc cggggagcaa
    27901 aaatgggcgg tggattccat tgccgactgc gcgcgtcgtt ttatggcgca tcctcaggtg
    27961 aaaggacgtc gggtatatgg acaggttggg ttcaactttg cggcgcatgc gcgggggatt
    28021 gcctttaacg ccggggagtg gccgctgctg acgttaaccg ttccccgtga agaacttatt
    28081 tttgaaaagg gaaatgtcac cgtttatgcg gactccgccg acgggtgccg acgtttgtgc
    28141 gagtgggtaa aagaggccgg tacaacgacg cagaacgcac cactggcggt ggataccgcc
    28201 ctcaatggtg aggcatataa acaacaggtt gcgcgcgccg ttgcggagat ccgccgtggc
    28261 gagtatgtca aagtgattgt ctcgcgcgcc attcccctgc catcgcggat tgatatgccc
    28321 gccacgctgt tatacgggcg gcaggcaaac acgcctgtgc gctcgtttat gttccgtcag
    28381 gaaggacgcg aagcgctggg ctttagcccg gaactggtga tgtcagtgat gggcaataaa
    28441 gtggtcactg aaccgcttgc gggcacccgc gatcgcatgg gaaacccgga gcataataag
    28501 gcgaaagagg cagaactgct gcacgacagt aaagaggtgc ttgagcatat cctttctgtc
    28561 aaagaagcta ttgctgaact ggaggctgtt tgccagccgg gcagcgtggt ggttgaagat
    28621 ttaatgtcgg ttcgccagcg cggcagcgtt cagcatctgg ggtctggcgt gagcggtcag
    28681 ctttcggaaa acaaggatgc ctgggatgcg tttaccgtgc tgtttccgtc gattaccgcc
    28741 tcaggtatcc ctaaaaatgc tgctctgaac gcgattatgc aaattgagaa gacgccgcga
    28801 gagctttatt ccggcgcaat cctgctgctg gacgatacgc gctttgatgc ggcgctagtt
    28861 ctgcgttccg tatttcagga tagccagcgc tgctggatac aggcgggggc gggaatcatc
    28921 gcgcaatcta cgccggaacg cgaactgaca gaaacccggg agaaattagc gagcattgcg
    28981 ccctatttaa tggtgtagga gtaactgttt tctaacctca caaccagact gtccaaagac
    29041 aattgagtcg ctctttgatt cctctgtgac tctattgtct tcttttggga taaaataggt
    29101 gtttttcgtc atgaagttga taaaagtcta tatagaacca cctcctgtgg aggtggtggt
    29161 aatttgccag agtgagggat tcgttagtat actatgggat aaacttattt ttaccaattg
    29221 tataaggcgg cgcataagat tctcggcatg cttccagata atccgaccac cactgcatca
    29281 tcgctttgcg ggcttctagg tgctctgcct tatgaatata agccatgcgc acggtattgc
    29341 gctcctgatg actcatctgg cgttctactg cgtcctttgc ccataaaccc gattccatca
    29401 gcgcactgca tgccattgcc cggaagccgt gaccgcagat atcctttttc gtgtcgtaac
    29461 ccatcacccg cagtgcctta ttgaccgtgt tttcacacat tggcttatac gggttatggt
    29521 cgccggggaa gatcagttca ttattaccgg tgatatcctt aatctgtttc agaatggcga
    29581 tggactgttc tgagaggggg acgatatgcg gcattcgcat ttttgccccg cggccggaat
    29641 aacgcacgcc aataatgggt tctcgcgtcg cgggtatcgt ccagactcgg tttgtgaaat
    29701 caatctctga ccagcgggcg aaacgcagtt cactggagcg aatgaacaca tgcagcatca
    29761 gcagaacggc atgccgggtc agttcacggc cctgatgata tgccccaata cgttcaagca
    29821 gttcaggcag ccgctccagc ggcagggcag gatagtgccg tctgacagga ggtgtggtca
    29881 cgccgccaag gtttgctgca gggttcgtat cgattaactc ctgatggacc gcatggcgca
    29941 ttatgttact caggtgctgc cgtgtgcggg acgcaacctc cagctttgag ataccagtgg
    30001 cgggagccac ccggcttgac cagcagatac agaccgtgag aatcggagac tttaaagggt
    30061 ttatcagaag gcttgagggt gcggattttt gcgtcggtaa ggaacatatg ggggtcactc
    30121 cattatcgaa ctaacctgat cccagatttg accaccaatt tttcccgatg catagggtaa
    30181 aatcaaaatg caccgagaag agttttcaca ctaacctgtt gaatcaacat cagataaaga
    30241 tttgcaagga tgcatgaaaa cagaaaattg gctcctctga ctggactcga accagtgaca
    30301 tacggattaa cagtccgccg ttctaccgac tgaactacag aggaatcgtg tgaacggagc
    30361 gcatattact tagcggtacc ttgtctgtca acactaaatt tcataagcca tttcaattgg
    30421 ttaattaatc tgcaaagttg ttaattaatg aacattcgtc gccgaaaacg agtctgtatc
    30481 attagcgtga tgcagtctct gcaaaggatc ttgttgataa aattgacaga aacgttgcca
    30541 caatgaaggg aaacgaggag caaaaagttc tggggcgcta aagaaatatt cagaaagtac
    30601 ggcaaaacat tcagcaggat cactggcagc ataagcatca atgctcgctg cattctcacc
    30661 aactaattcg atttcttcct gaatgttgtt cattgcagca tgaagatcgt gttcccagcc
    30721 agcaacctca cgcaacgaaa taaagggaac tccgctggcg cgatcgccgt tacgggtgtc
    30781 cagcttatga gcgacttcat gaataatcag gttaaaacca gatgcatcaa aagaatcttg
    30841 tatatccaac cagttcaaaa cgataggccc ttgctgccag ctctgacctg actgaacaat
    30901 acgttggtta tgcaccagac cgatatcgtc ttcccattca tcatcgacca caaatggcgc
    30961 aggataaatt aagacttcat gaaaaccatc cagccattcc agtcctaact ccagaacggg
    31021 taggcaaaat agaagtgcta tccggcagct tcttaatgaa tccagttcaa agccctgtaa
    31081 aggaacaagc cgcttttgct gtaaaaaacg ttcggcaaga gtgactaatt tgctttgttc
    31141 ctgttccgtc agacccgtta aaagggggat cgatagtgct tcctgccagg gaagggcagt
    31201 ttgatgtgct gattcttgta ctttccaggg ccacttaatc atcgttttgc tcgcaaactc
    31261 gtcacttgaa caaaattgca cggacaggga ctgttaaaat gccaaatttc ctggcatcat
    31321 ggcaaccatc tgaacggaga gatgccggag cggctgaacg gaccggtctc gaaaaccgga
    31381 gtaggggcaa ctctaccggg ggttcaaatc cccctctctc cgccacttta tcaatgactt
    31441 atctcctgac ttcccgcctt gcttttccta aacagaacaa tcgtagaata ttcttgaagg
    31501 gttagatcgt cactgttttc tgttcgatac tgtgacattc agcacttgat tcgctatgga
    31561 gctgacagga aggtttcgag cgaaaatcta cagttattca gtcgttttct tatcggtcac
    31621 cattattctt ttagacattg atcctatgaa actgccgcaa agttgctggc gggaactgaa
    31681 gttgcgtaga gaaggggtca ataattgagt caaaaaagga acgcggccgt cagattaaag
    31741 ccgcgggaca aagtccataa tttccaagag ttgttgaatt tcccagcttt tttgaaagcc
    31801 tttgtatcat actaacaagt aaaaaaggtt taaacaccaa aattaaccta tatttctggt
    31861 tagctttacc atgacagatg acttgctcct ggaaacattt gcgctgccag tctgtatggc
    31921 tgagttgtgt atttattgtt cgcctttttc gcattttttc tacagacata ataaaaaaga
    31981 cacgactcaa gtatgagcgt gtctgagttt gtcttactta tttgtcttgg caaattattg
    32041 ttgttactac caggcactgt tctgttaaat aactgaagtc agcgcaaaaa aatcaatgag
    32101 acatcatctc ctcgaccact tcttcgctac tcaactgata gggataatac gttggccagt
    32161 tttccatttc attcaataaa gactgttggg aatcattacc cataaaaatg tgaaaatgag
    32221 atgattttcg tggtgcaatt atatgatcgc taaattgtat atatttaggg gctttacttt
    32281 caggatcttt acattcgaat aagtaacgaa cgcctttctt gcctgattta taggtgagta
    32341 ttttgtatcc atcgtaatca tatttacagg atgttgtttc attatttcta tggaattcga
    32401 caatgccgtc ctcaatgcca atcatctcga tgtctgtcac ataacctttg cgataataat
    32461 ctttaatttc agcaaatgtt ttggttttat ctgcatccgc tttcttctga aagatggggt
    32521 caagtttgcc actttgcagt aaaggataaa cggattgcca gactccatcc cagtcactga
    32581 gcgttcggtt ttgtacattg gcatcatcaa aaacaccatt agcagctttt tgttcgacct
    32641 ctgttaaggg tttgccgtgt gagtgatgac catgcgaaaa ggcaggagcg ctaacaataa
    32701 agacacctaa agcaacagcc agtttatgaa gacgaatcgc caaaacagtt tcctccagag
    32761 ttaatgtata tgatgtgtaa tgttatattg taacatataa caaaatgcaa caactcttag
    32821 gagacaagag aatagttctc tcaggtgtgc ttatctatct tattgaaata aaataaatta
    32881 tgtcgatgat ttgaggttga tgacaaacga aacataactt gatgtgatct gcgtaggccg
    32941 gatagggcgc tcgcgccgca tccggcaggg ataacgcgta ctttgtcggc aatatgaagt
    33001 tatacagata atttgttgtg aacctgcttg cgtaaccggt taaaaaggga aagcaacttc
    33061 ttataccgta aggctaaaag cagtaccgcc agcccagcgt agatgagtgg ctgcggtgaa
    33121 ataatcttca ctgaccacaa ataatgtatt ggggccagga tcgcgacaag atagacgaag
    33181 ttatgcaact gttgccagtg tttaccgagt tttcgctgca tcgcctgggt tgaagtgaac
    33241 gctaacgcaa gcaaaattac ccagctgata atacctaacg ttaaataagg ccgggtaatt
    33301 aactccttac ctaataacgc cagattattc acgcccagct ccagcaatgc gtaactggtt
    33361 aaatgcagtg tcgcccaggc aaagcaccat aagcctaaca ggcggcgagt gcgtatcaat
    33421 aacggctgtt ttgcgtagcg tgccagaggt gtgattaaca aggtcgccag caaaaatttc
    33481 agtgcagtgc gaccagtaaa atgctgaata tctttcaccg gatcggcacc caatccaccg
    33541 tgattaatcg cccagaccag ccagagaaac ggcaacaatc cggcaagatg caggcaaact
    33601 ttcagccatg tcacctgttt tgctgtcaga cgcatttaga aattctcccg caaatccagg
    33661 ccacgataca gcgatgccac ctgctcggcg taaccattaa acagtagcgt tggctggcgc
    33721 tgtacatcga ggatgccgcc tgaaccaata aatcgttcgg tagcctgtga ccagcgcggg
    33781 tgatcaacat gcggattaac gttggcgtaa aaaccgtatt cgtcaggcgc tgccagattc
    33841 caggtggttg gcggacgctc gcgggtcagc ttaatactga ctatcgattt aatcccttta
    33901 aagccatatt tccacggcac aatcagtcgc accggcgcgc cattttgtgg cggtaacgcc
    33961 ttgccataaa cacccacggt catcagtgtg agcggatgca ttgcttcgtc gagacgcaat
    34021 ccttcgacat aaggatattt cagcccgcca ccgataaagc ggtcctgctg gcctggcatt
    34081 tgttccggtg cataaattgt ttcgaaagcg acatacttcg cattgctggt gggttcggca
    34141 agcgccagca atttgtgcag cggaaaacca atccacggca ccaccatcga ccatgcttcc
    34201 acgcagcgca tacgataaat acgctcttcc agcggaaagc gacgggttaa atcatcgtga
    34261 tcgagggtca atggttttgc cacttcgccg ctgattttca gtgtccatgg atcggttttc
    34321 aggctaccag cattagcggc gggatcggct ttatccagcc cgaattcata gaagttgtta
    34381 taaccggaga ctttatctgc tggcgtcagt ggcaggtcat tttgccaggc ggcaggcttg
    34441 ctgaactcca acggttttcc ggcgggggcg ggtgggcgat cattcccttt gaaccagcta
    34501 agcagatcgg catgcgcagc gtgaggcaaa gaaagtgcag ccgcgctgat gcccagtgct
    34561 tttaacacct gccgacgcgt cataaagaat accgactcgg ccgtaacatc tgattctttt
    34621 aaaaattgat tctttttcat aagccatcac acttttttgt ctttagccag caagagccgt
    34681 ttactgttaa tctgtcagga agtgtcctcc ctaacagtag cagagatctg cttttacata
    34741 tttatgcatt aactgccacg ataggttgaa tacccatatt ggctaagtaa taaaggcaca
    34801 tgataatgct cgttcacttt attaatatga aattcaacgg ggatctcagg gaagaaactt
    34861 tcaagatttt gtttcttgaa atagtccccg gttttaaata cgacacggta atcgcccgta
    34921 gttgcagttt gctcgggcca cagtgcctta attcgtccat ccttatctgt tttggcggta
    34981 ttaagttgta accagccgtt gtccgctttc ttttcaagag tgactgtcac gtcggcagcg
    35041 ggttttcctg tttgctggtt caaaatgtgc acactaagaa tgttttgttg tgcagcatag
    35101 accaaagaag gtaatgaaaa tgcagccgtt actacggaga gtactaaata acgctttaac
    35161 atgtttatat ccttgtcatg tgaatgagtt ttgaggatag cgaaaaagcg tccacggtaa
    35221 ccttacagca gcattacaat tttgtaatga accaggctgt ttctataaca tatgatttat
    35281 ggcatattat tttcatgaag attttactta ttgaagataa tcaaaggacc caggagtggg
    35341 taacgcaggg gctttccgaa gcgggttatg tcattgatgc cgtttctgat ggcagagatg
    35401 ggctttatct tgcgcttaag gatgattatg cattgatcat tctggatatt atgcttccgg
    35461 gtatggatgg ctggcagatc ttacaaacgt taagaacagc aaagcaaact cctgttattt
    35521 gccttactgc aagagattct gtcgatgaca gagtcagagg gctggacagt ggggcaaatg
    35581 attatctggt aaaacctttt tcattttctg agttgctggc aagggttcgg gcacaattaa
    35641 ggcaacatca cgctttgaat tcaacattag aaatcagtgg cttaagaatg gactctgtta
    35701 gtcaaagtgt gagcagggac aatatcagta ttacactgac gcgcaaggag tttcagttac
    35761 tttggctact ggcctccaga gctggcgaga ttatccccag aacggttatt gcgagtgaaa
    35821 tttggggaat caactttgat agtgatacca atacggtgga cgtcgccatt cgcaggctcc
    35881 gcgcaaaagt tgatgatcct tttcctgaaa agctaatcgc cacaatccgg gggatgggct
    35941 attcattcgt agcggtaaaa aaataatgaa aagattatct ataaccgtcc gtttaacctt
    36001 gctttttata ttgctgctat ctgttgctgg cgctggaatt gtatggactc tctataatgg
    36061 cctggcaagt gagttgaaat ggcgcgatga tacaacactc attaaccgga cagcgcagat
    36121 caagcagttg ttaattgatg gggtaaatcc agatacgtta cctgtgtact ttaaccggat
    36181 gatggatgtt agtcaggata tcttgatcat tcatggtgat agcatcaaca aaattgttaa
    36241 ccggacgaat gtcagtgatg acatgttaaa taacatacct gctactgaga caatcagcgc
    36301 agctggcatt tacagaagca ttattaatga tacagagata gatgctttac gaattaatat
    36361 tgatgaagtt tcgccatcat taacggttac tgtggctaaa ttggcttcag ccagacataa
    36421 catgcttgaa cagtataaaa ttaatagcat tataatttgc attgtcgcca ttgtactttg
    36481 ctcagtatta agtccgctgt taatcagaac gggattacga gagatcaaaa agttgagtgg
    36541 tgtaacggaa gcgctgaatt ataacgatag ccgggagcct gttgaggtta gcgcattacc
    36601 gagagaacta aaacctcttg ggcaggcgtt gaataaaatg catcatgctt tagtcaaaga
    36661 ttttgagcgt ctaagtcagt ttgctgacga tctcgctcat gaacttagaa cgcccattaa
    36721 tgcattactg ggtcagaatc aggttacgct cagtcaaacc agaagtatcg ctgaatatca
    36781 aaaaacaatt gccggtaaca ttgaagagct ggaaaatatt tcgcggttaa cagagaacat
    36841 actgtttctt gcccgggcag ataaaaacaa tgttttggtg aaactggact cactttttct
    36901 caataaggaa gtcgaaaatt tgttggatta tcttgaatac ctttcagacg agaaagagat
    36961 ttgctttaag gtcgagtgca atcagcaaat ctttgcggat aaaattttac tgcaacgaat
    37021 gttatcgaat cttattgtta atgccattag atattcacca gaaaaatcgc gcattcatat
    37081 aaccagtttt cttgatacca acggctatct taatattgat atcgccagtc ctggaacgaa
    37141 aattaatgag cctgaaaaac tcttccgtag attttggcgg ggagataatt cgcgtcattc
    37201 cgaaggtcag ggacttggtc tttctttagt caaagcgatt gccgaattgc atgggggaag
    37261 tgctacgtat cactatttta ataagcataa tgtgtttcga attacgttac cgcaaagaaa
    37321 ttaacatagg gcttcagtac gccataaatc aaccatgaaa aaacgcagcc acgtcgtgag
    37381 tattaacgtg gctgcgattg attatgcgct tacattcata cgtaacaggg attaacccgc
    37441 ataagctgcc agcatttcct gcgccgccag tttacccaac gcattcgctg cgaaaggagt
    37501 gtcgccggtg agaactttac ggtccttatg tactcgcccg gtgatgtcgt cattaataat
    37561 gttcatgccc attttcttca gctcttcgcc gaagtaccag gtgagatgac ccggcatata
    37621 gcctatctct ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg aataaccatt
    37681 cagtgggtta tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc aaagggagat
    37741 aacaaaacgg tcatttttga ttgcccactg caaagcagca gccacgtcct ggctttctgg
    37801 taaaccaata agtgcgccat gaccaccagg aacaaagatt gctgcatatt cgctgtctgc
    37861 gttgaggctg acgacaacat ccgcgatttt tttcggattg cggaacaacg atttatgctg
    37921 ctcaaagaat ggcattactt tttcatcttt gtgcggcata gcccagtatt caaacttggt
    37981 catcagaccg gaaatggtcg ccacttcgaa ttcgaaacct gcagcatgga gatgatacaa
    38041 cggcagcaac gtttcaatcg gatggttacc ggtcgagaac agttttccgt tatcggtcgg
    38101 caaataacgt tcgtccgcgg cgatcaccag aattttatgt ttaccgcgat acggttttg
//
LOCUS       DAZFKM010000032        37462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFKM010000032 DAZFKM010000000
VERSION     DAZFKM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..309)
                     /gene="yiiF"
                     /locus_tag="ACHKAY_001762"
     CDS             complement(67..309)
                     /gene="yiiF"
                     /locus_tag="ACHKAY_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO5700450.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(528..746)
                     /gene="yiiE"
                     /locus_tag="ACHKAY_001763"
     CDS             complement(528..746)
                     /gene="yiiE"
                     /locus_tag="ACHKAY_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO5700451.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1351..1641)
                     /gene="nadS"
                     /locus_tag="ACHKAY_001764"
     CDS             complement(1351..1641)
                     /gene="nadS"
                     /locus_tag="ACHKAY_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO5700452.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1642..1953)
                     /locus_tag="ACHKAY_001765"
     CDS             complement(1642..1953)
                     /locus_tag="ACHKAY_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5700453.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2182..3090
                     /locus_tag="ACHKAY_001766"
     CDS             2182..3090
                     /locus_tag="ACHKAY_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5700454.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3154..4095)
                     /gene="fabY"
                     /locus_tag="ACHKAY_001767"
                     /gene_synonym="yiiD"
     CDS             complement(3154..4095)
                     /gene="fabY"
                     /locus_tag="ACHKAY_001767"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO5700455.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4140..4577)
                     /gene="dtd"
                     /locus_tag="ACHKAY_001768"
     CDS             complement(4140..4577)
                     /gene="dtd"
                     /locus_tag="ACHKAY_001768"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO5700456.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4574..5446)
                     /gene="yihY"
                     /locus_tag="ACHKAY_001769"
     CDS             complement(4574..5446)
                     /gene="yihY"
                     /locus_tag="ACHKAY_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO5700457.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEDDDEP"
     gene            complement(5440..6039)
                     /gene="yihX"
                     /locus_tag="ACHKAY_001770"
     CDS             complement(5440..6039)
                     /gene="yihX"
                     /locus_tag="ACHKAY_001770"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO5700458.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEVF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6138..6923)
                     /gene="csqR"
                     /locus_tag="ACHKAY_001771"
     CDS             complement(6138..6923)
                     /gene="csqR"
                     /locus_tag="ACHKAY_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5700459.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6957..7853)
                     /gene="yihV"
                     /locus_tag="ACHKAY_001772"
     CDS             complement(6957..7853)
                     /gene="yihV"
                     /locus_tag="ACHKAY_001772"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO5700460.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            8021..8917
                     /gene="yihU"
                     /locus_tag="ACHKAY_001773"
     CDS             8021..8917
                     /gene="yihU"
                     /locus_tag="ACHKAY_001773"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO5700461.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8941..9819
                     /gene="yihT"
                     /locus_tag="ACHKAY_001774"
     CDS             8941..9819
                     /gene="yihT"
                     /locus_tag="ACHKAY_001774"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO5700462.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9836..11077
                     /gene="yihS"
                     /locus_tag="ACHKAY_001775"
     CDS             9836..11077
                     /gene="yihS"
                     /locus_tag="ACHKAY_001775"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO5700463.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            11215..12117
                     /gene="yihR"
                     /locus_tag="ACHKAY_001776"
     CDS             11215..12117
                     /gene="yihR"
                     /locus_tag="ACHKAY_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO5700464.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            12316..14352
                     /gene="yihQ"
                     /locus_tag="ACHKAY_001777"
     CDS             12316..14352
                     /gene="yihQ"
                     /locus_tag="ACHKAY_001777"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO5700465.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14398..15783
                     /gene="yihP"
                     /locus_tag="ACHKAY_001778"
     CDS             14398..15783
                     /gene="yihP"
                     /locus_tag="ACHKAY_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700466.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15826..17229
                     /gene="yihO"
                     /locus_tag="ACHKAY_001779"
     CDS             15826..17229
                     /gene="yihO"
                     /locus_tag="ACHKAY_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700467.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            17297..17989
                     /gene="ompL"
                     /locus_tag="ACHKAY_001780"
     CDS             17297..17989
                     /gene="ompL"
                     /locus_tag="ACHKAY_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO5700468.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18080..19345)
                     /gene="yihN"
                     /locus_tag="ACHKAY_001781"
     CDS             complement(18080..19345)
                     /gene="yihN"
                     /locus_tag="ACHKAY_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700469.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(19447..20427)
                     /gene="yihM"
                     /locus_tag="ACHKAY_001782"
     CDS             complement(19447..20427)
                     /gene="yihM"
                     /locus_tag="ACHKAY_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO5700470.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(20435..21145)
                     /gene="yihL"
                     /locus_tag="ACHKAY_001783"
     CDS             complement(20435..21145)
                     /gene="yihL"
                     /locus_tag="ACHKAY_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5700471.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(21362..23185)
                     /gene="typA"
                     /locus_tag="ACHKAY_001784"
     CDS             complement(21362..23185)
                     /gene="typA"
                     /locus_tag="ACHKAY_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO5700472.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23558..24967
                     /gene="glnA"
                     /locus_tag="ACHKAY_001785"
     CDS             23558..24967
                     /gene="glnA"
                     /locus_tag="ACHKAY_001785"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO5700473.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            25286..26335
                     /gene="glnL"
                     /locus_tag="ACHKAY_001786"
     CDS             25286..26335
                     /gene="glnL"
                     /locus_tag="ACHKAY_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO5700474.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            26347..27756
                     /gene="glnG"
                     /locus_tag="ACHKAY_001787"
     CDS             26347..27756
                     /gene="glnG"
                     /locus_tag="ACHKAY_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO5700475.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27868..27978
                     /gene="yshB"
                     /locus_tag="ACHKAY_001788"
     CDS             27868..27978
                     /gene="yshB"
                     /locus_tag="ACHKAY_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO5700476.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(28168..29541)
                     /gene="hemN"
                     /locus_tag="ACHKAY_001789"
     CDS             complement(28168..29541)
                     /gene="hemN"
                     /locus_tag="ACHKAY_001789"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO5700477.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29730..30239)
                     /gene="yihI"
                     /locus_tag="ACHKAY_001790"
     CDS             complement(29730..30239)
                     /gene="yihI"
                     /locus_tag="ACHKAY_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO5700478.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(30358..30524)
                     /locus_tag="ACHKAY_001791"
                     /pseudo
     CDS             complement(30358..30524)
                     /locus_tag="ACHKAY_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30821..31453
                     /gene="yihA"
                     /locus_tag="ACHKAY_001792"
     CDS             30821..31453
                     /gene="yihA"
                     /locus_tag="ACHKAY_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO5700479.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(31835..34621)
                     /gene="polA"
                     /locus_tag="ACHKAY_001793"
     CDS             complement(31835..34621)
                     /gene="polA"
                     /locus_tag="ACHKAY_001793"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO5700480.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            34985..35917
                     /gene="yihG"
                     /locus_tag="ACHKAY_001794"
     CDS             34985..35917
                     /gene="yihG"
                     /locus_tag="ACHKAY_001794"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO5700481.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(35958..37388)
                     /gene="yihF"
                     /locus_tag="ACHKAY_001795"
     CDS             complement(35958..37388)
                     /gene="yihF"
                     /locus_tag="ACHKAY_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO5700482.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         9190 a         9552 c         9059 g         9661 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca cgttgtcagc aatctgattg acatcatttt
       61 ttcccattac cattcctcgc gcagcttacg ctgatattcg acaccatctt cctcacaccc
      121 ttgccagatg ccaggaacac tggttcttgc tgtttgcgat tgatttatca ggtattgatc
      181 taccgcttcc cttaataggt ccgcacgagg aagattacgc tgcacctcga gatcatcaag
      241 ttgcttaatc acctcattcg ataaattgag taaaattctg cccatatcta tacctgccag
      301 agagttcata aatatctcgc caatatcatt tttgaatcta tggagagaaa agtacccttg
      361 ccgaatcttt aaagaaagct catttacgca tcatttttta tttttgcgag gcgttttcca
      421 gatacgatgt aacagttgca gttattataa gttcaagtaa aacagccttg tattaatacc
      481 agaataaggg catctgtttt tttatattca agaatgaaaa atttttttca ttccttatgc
      541 tccttacact gatcatcaac accaactacc tccccctttt gcctgcctcc ctttgcttta
      601 cgcaccggaa attgctgccg ctccagatat tgctcaaccg cttcgcgtaa aattgcagat
      661 cgtgaaacct tacgccagcc ccgaagtttg ttcagccgtt taattgcctc ttctgataaa
      721 tgaagaaaaa ccgtattcat cgccattacc accaccttac ctatattact cattaatgta
      781 tgtgcgaaaa ttacgccgga aaatcattta aaaaaagcgg atttttgcat gttgttttgt
      841 ttttgcgagg cgttttccag aaataataag agtgttgcag ttgtttcatt tattactcaa
      901 aaatggttta gttatcgccc aggatgagtt atggatgcta tgaatatatt atattcaggg
      961 atgtttattt tttgattgga tctttatttg cataatctga atggtgtacc cgaaaaatgt
     1021 cagcatccta acagcacccg gatttaaggt gaaattaatc atccatcaca gaccggtcag
     1081 tactgccacc aaccggtctt tacttgttca tcgttataat tttgttggta acagttcggg
     1141 aaacaaggaa ttccttccgg aaccccccgg tatcatccga tagcattgac gtcaatcatc
     1201 agcgcgacga tgagtttttt taatcgcatc atagggtggc gacgttcagc acgattacgg
     1261 gtcatagcaa aactcctggg caggcgacca ctatgccgct ttcacaggag ttctgaatgc
     1321 tagtgctttc tgtatggcat aaccatgcct ctaacggtta agtgcttgca gcacatgttg
     1381 cggatcattg gcaatcgctc gcagcaaagc ctttgcaggc ccggtcggtg aacgtcgccc
     1441 ctgctcccag ttacgcaacg tgtctacgct tacagaaata agatcggcaa atttagattg
     1501 tgacaaccct gaagcgcggc gtatttcttt gattttcata gcatcaactt caaaagtccg
     1561 cgaaggctgt cgcgttcctt caataatttc attcatctgg gccatgctgt gagtcaaccg
     1621 ctcaaaaagt gccttatcca tttaccacct cgtatttaac aaccggagca ttgctttttc
     1681 ctggggagac agatcatctt taataccctt acgataaata agcagtaatc tgatctcata
     1741 atgtttaacc tgatgaaagt aaataaccct gacaccagca cgtttcccct ttcctcctga
     1801 cacccatcga acctttctta acccaccagt ttcaggtatt acctcaccat agtcaggatt
     1861 caaggccagg aaaaactgaa aacgactgaa ttcatcgtcg gtcagcagtt tttgaacatc
     1921 ttcagtaaaa atttccgttt cgatgaagag cataattata cgccattggc ttagagtaac
     1981 aatccttttt gtacgtcatt gacgtaccct tgaaaccact agcttactga tagtgctcca
     2041 gcaaaaccac gtctctgccg ggtttgtctt tagatttgcg aggcgctttc cagatttttc
     2101 acgcccgtga atttccctgt atccatccag caaaaacggg tgtataagac tgtacattca
     2161 tacagcaaaa taagttctac tatggcactg gaaaaaggaa ttgcagagtt agtcgaaaag
     2221 tttatcgctg cgggtcgccc ctcatcgcgt caacaaagta ttgcgcagcg cagggagggc
     2281 tatatcgcca gcgctgtcct tgcgggtgag acagaaacgc gggtagatat tcagactatc
     2341 gaacttgaag ggatgacctt acgaatagtg tcgccgctga atgccccgac attactcccg
     2401 acgataattt attactacgg tggctgtttt gttagcggcg ggttcgcaac tcacgacaac
     2461 cagctgcgcc agttagccta ttacgggcaa tgtcgggtta ttgccgtcca gtatcggctg
     2521 gcaccggaac atactttccc cgctgcccat gatgatgcgc aacgaggtgc agaaatagtc
     2581 tggcaacacg ccgaacgact cggcgttgat aaacaacgta taactcttgc cggtgacagt
     2641 gcgggcggac atctggcgct ggtaactgct ttgcgattaa agcgtgctgg cgagtggcaa
     2701 ccggcacaac tcatcttaat ttatcctatg cttgatgcga ctgcgcattt cgaaagctac
     2761 atacgcaatg gtcacgatta catcatcaca cgcgacactt tactgagcgg ctttgagatg
     2821 tatctgccag gcattgaacg caggcatcct gaagccagcc ctctttggcg cgatgatttc
     2881 aatggcttgc cacctgtaca tatcattacc gctgaatacg acccactttg cgatgaaggt
     2941 gaagcgttgt atcaccgcat gacagggcaa ggagtgcaat gcacctgcca acgctacctt
     3001 ggcgttatcc acggattttt tcagttaggt ggtatcagtg aggccgcgcg atcggcacta
     3061 cgggatgttg cctggagagc gggaaggtaa cttcggacct gggccctgaa aaggttttca
     3121 gattgaaaac cttttcagtc aagtaaaacg cccctactct tcttcgttcc cgccctcttc
     3181 atacgggcca aatggcttcg cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg
     3241 cgtctcgtcg ccaaagattt caacttgcat ctgcacccgt gcttttcgtc cgcgcgccag
     3301 acggtcgaga tcgccgctta aggcaccaag gtcggctacc gcatgaggtt taccgctaat
     3361 cggcttgctg tagcggatat gcgcatccgc cagaataatc gttccgccga gatggcgttc
     3421 gcgcagcatc agccagataa gcccccaacc ggtgagcgtt gccagtgaga ataaactccc
     3481 ggcaaacagc gtatggtgcg gattctgatt accggtttcc ggcatggtgg tgataaattt
     3541 ttgcccggta tattgttgaa tacgcacgcc cattttttca ctaagcggga tatgttcgta
     3601 ccacgcctgt tgcagctgcg cgcaccagtc gccgcgatgc aaaatatcat ccagagtggc
     3661 gacgggctta atcatcaaaa aatggcgaat cggcgtggtg gttggcgtgg tgatttctcc
     3721 ctgattaaca aaccccagct tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt
     3781 cacgcgctta acgccttcct gacgcgccac cgactccagg gtcatcgcca tcagcgttcc
     3841 taaccctttg tcctgcacgt ccgggtgaac ggccataaag cgaatggacg cttcattgtc
     3901 ggcattaata tacaatcggc ctaccgccac cagattaccc tgctcgtcga cgaccatctg
     3961 gtgatgcgcc atcgcatccc acgcgtcgcg ttccgaacct tttggttgat gcaggggctt
     4021 acgcaacatt tcccagcgaa actgatagta acgctctaac tcttcttctg tttgtggaac
     4081 ccgaaggtga tacatagcga tactctctct tgtttcccgt gacaaccctg gaagctggct
     4141 catacctgca accagaatgt cacggggcca tcattgacca gcgatacctg catatcggca
     4201 gcgaagcgtc ctgtttgcgt gttcatctct tgctggcggc agcgttcgac gaaataatca
     4261 tataacgcct ctgcgcgatc cggtgatgcg cctttagaga aacttgggcg catcccccgt
     4321 tcggtatctg cggcgagggt aaactgggaa accaccagta cactgccgcc cgcctgttgc
     4381 acgttgagat tcatcttgcc ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg
     4441 cacagacggt ttgctttctg ttcgtcgtca tccttttcga cacccaataa caccaaaagt
     4501 cccgcgccaa tttcgcccgt cacttctccc tccacggtga cgctggcacg ggttacgcgt
     4561 tgaattaatg caatcatggt tcgtcgtcgt cttcttgttc agctgcttgt tttagtttgc
     4621 ggtattcccc gagagtgaca gtaatttccg cgccaagcaa gacgatacac catgtccagt
     4681 agacccaaac aaagagaatg gggatcaccg ccaacacgcc gtaaatgagc tgatatgacg
     4741 ggaacatggt gatataaagc gcgaaacctt tctttcctgc ttcgaacagg agcgcggcga
     4801 caaacgcgcc gacaatcgcg tcgcggttag gtacgcggat ggtaggaaca atgctgtaca
     4861 gcaaccagaa ggagatccac gacaacagca gcggaaaaat acgcagcacg ttatcgatga
     4921 cagtattgag atcgctcgcc cagcgcaggg agagcaaata ggaactgatc gccagactgg
     4981 cccctgccag cagcggcccc agcgttaaaa tcatccagta cacggcgaac gagtaaattt
     5041 ttggtcgcgc tcgtttactg cgccagatgg tattcaacgc gctatcgatg gagtacatca
     5101 gcaataacgc ggtgacgatc agcccgcacg ccccaacggc ggtcatcttg ttggaattgg
     5161 caacaaattg ttcgatatac cgctgaataa catcgccagt agcaggcaga aagttggcaa
     5221 aaataaagtg acgtaactga atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg
     5281 caaaaacaac ggcaaccagc ggcactaatg agagcaacga cacataggca aggttacctg
     5341 ccagggttgt catgttgtcc tcatcaatac gttgccagag tagttttagc caggcccata
     5401 gtggacgggt acggtgcctg gctttgtcct gaatggtttt tagcataaca ccttcgcgaa
     5461 atagtccggg atggtggttt tatctttcac cagaatactg gtaatgccca gctgattggc
     5521 tccttctata ttatcggcgt tatcgtcgaa aaagaccgta tcgctgggtg aaaaaccttc
     5581 cgcctgcaaa acatgctggt aaattcgtgc ttcaggtttg cgcatcccca gatcttgcga
     5641 cagatagata tggtcagcag catcacgaat ttccgggtat tcttccggcc agaaggtggt
     5701 atgcaggcgg ttagtattgg aaagcaccac cacgcgatgc ccctgctcac gcagtttatg
     5761 catgatggcg atcacttccg ggcgcagcgc aacaaacacc gcctgccagc cgtgagagaa
     5821 ctgctcgtag cttagcggca gggccatctc atgacacagc gcctctgcga acgcttcgtc
     5881 gctaatttcc ccacgctcat gctgatgaaa cacctccccc atgtggaaac tcttcttaag
     5941 cgtagccagt ggaacacgcg ttaaatcgct ccaggctccc agcacacggt taaagtcgat
     6001 atcgacaatc acattaccta aatcaaagat atagagcatt tttgcctcct ttcgcgccac
     6061 aagaaattaa ctgtagcggg aaaggatggg tttggctatg tggcaaggta tgaagccagg
     6121 ttaacggtca caaaaatcta cgcgtcttcc tggggaagaa tgatttcaat ttggctgtct
     6181 tgtaagcgtg attttagcgc agcgggcggc agctcgtcgg taaagagcgc agtgacctgt
     6241 gccacgttac caatttcaac cgccgccgaa gcatgatact tagtgtgatc ggcgaccagc
     6301 agaatatttc tcgcgtgcgc catcatcgtt ttcaccacgt tagcttcgtt tacatcaaac
     6361 tccatcaacg cgccatcgct ctcaatcgcc ccaacgcttg ttaccagata atcagcacga
     6421 aaatcagcca caaaggacgc cgctgaaggg ccaatgatcc cgctattatg agagcgcaac
     6481 gtaccgccgg gcaccatcac ttcaaagcgc gggttgtggt aaagaatatg cgccacacgc
     6541 aggctgttgg tgattatccg caaatgatta tggttaagta acgcccgggc aacatgctca
     6601 acagtcgtac caatggtgat aaatattgtt gaaccatcag gaatatagtc tgccaccgct
     6661 tcggcaatcg cttttttttc ctcggtttgc gaaacctcac gctgctcgaa cgccgtatta
     6721 acgacgctgg aagcccgacc cgcgccaccg tgatggcgcg taatcaggcc ttgctcgctt
     6781 aatttacgaa tatcccggcg gaccgtctgc gtggagacat ccagcagatt tgccagctca
     6841 tcaatattca tatacccacg ctcggcgatc agcatgagga gttggtcgtg ccgcgggtta
     6901 ccggttagtt cggtaaggct catgaaaatt cctcgaaaaa ccatcaccct ggcattttat
     6961 acaaaaagtg acaaaaaaga tcgggtttga tcacagtcag ggatcccggc tcgtccaccg
     7021 ggacgtgtgc attttaacgc cgctacaccg ctggcgaagc ggactgactc cgctaaatcc
     7081 ccacttgttg ccagcgccac cgccaaagcg ccgtgaaaaa catcacccgc acctgtggta
     7141 tctaccacat caactttgaa ggccggttga tgctgacgcc caccattttc cagccagtcg
     7201 cagcctgcgc taccctgggt cacatagaca tgaccatttg tgagcgtttg tgcctgtttt
     7261 agcgcactgg ccatctcttt cacgcccgtt aagcgcgcca gacccggttc tgaaaaggcc
     7321 gcgtgatcgc ttaatgccac cagctcactg atatcctgcg gcgtaatgtc cccgtccaga
     7381 acggtcatca cacccgcctg acgggccagg gtgaaggctt ttttagcgcc gtcgtgccag
     7441 cgtacatctg ccagcacaac atcccactga gagaaatcaa tttcctccaa ccactctgcg
     7501 tcaggcagca gatccgggct ggggtagtta atgattatcc gctcgccttt ggtatccacc
     7561 atgatggcgg attgcgaaga tttcgcctgg ttataccgtt tggtgtaacg ggtgttaacg
     7621 ccccaggatt ccagctctgc cagcaggctg ttgcccgtgt cgtcatcacc tacgcgacca
     7681 ataaaatcga cctgcgcccc cagccttgcc gccgcaaccg ctgccgtcgc cgctggcccg
     7741 ccaccaactt ccgtataatt tctcgccacg tatttaccgc tctccgtcgg taacccttcc
     7801 acgtaataga tgcgatccat cacggttata cctacacaag caacacgaat catggtcatt
     7861 ccttaaacat tttgggatgc gttcatttta atttcaccaa ttgtttaaaa agtgacggct
     7921 gtcaattttt tgacgataaa ttacaaatac gatcaaaaac agacaagcaa agcatgtcat
     7981 aaatgacaaa aagtgacatc actgtattca ggagagggtt atggcagcaa tcgcgtttat
     8041 cggtttagga caaatgggtt cgccaatggc gagcaattta ttgcagcaag ggcatcaact
     8101 tcgcgtcttt gatgtgaacg ccgaagctgt gcggcatctg gtagacaaag gtgcgactcc
     8161 cgccgccaac ccggcgcagg ccgctaaaga tgccgaattt atcattacca tgttgccgaa
     8221 tggcgatctg gtgcgcaacg tgttgttcgg tgaaaacggc gtttgcgaag gcttatctac
     8281 cgatgcgctg gtcattgata tgtccaccat ccatccgctg caaaccgata aattgattgc
     8341 cgatatgcaa gccaaaggct tcagcatgat ggatgttccg gtaggccgta cttctgcaaa
     8401 tgccattacc ggtactctgt tactgctggc tggcggcacc gctgaacaag ttgaacgtgc
     8461 cacaccgatc ctgatggcga tgggcagtga gttgatcaac gctggcggtc cgggcatggg
     8521 gatccgcgtt aagctcatca acaactacat gagcatcgcg ctcaatgcgc tttcggcaga
     8581 agccgccgtt ttgtgcgaag ccctgaatct tcccttcgat gttgccgtca aagtgatgag
     8641 cggtaccgcc gccggtaaag gtcacttcac cacttcctgg ccaaacaaag tcctcagcgg
     8701 agatctttct cccgccttca tgatcgatct tgcccataag gatcttggca tcgcccttga
     8761 tgtcgccaac cagctgcatg tgccaatgcc gctgggggcc gcctcacggg aggtttatag
     8821 ccaggcgcgc gcagcgggtc gcggtcgcca ggactggtcc gccattctgg aacaggtccg
     8881 tgtcagtgcc gggatgactg ccaaagtaaa aatgtaacga ctggataaag gaataaatga
     8941 atgaataagt acaccatcaa cgacattacg cgcgcatcgg gcggttttgc catgctggcg
     9001 gtcgatcagc gcgaagccat gcgcatgatg tttgccgcgg ctggggcacc cgctccggta
     9061 gccgatagcg ttttaactga tttcaaagtt aacgctgcaa aggccctctc gccttatgcc
     9121 tcggcgattc tggtagatca acaattctgc taccgccagg tggttgagca aaacgcgatt
     9181 gccaaaagtt gcgccatgat tgtcgccgcc gatgagttca ttcctggcaa cggtattccg
     9241 gtcgatagcg tggttattga ccgcaaaatc aatccgctac agatcaaaca agacggtggc
     9301 aaagccttaa aactgctggt gctgtggcgt agtgatgaag atgcgcagca acgtctggat
     9361 atggtgaaag agttcaacga actgtgccac tcacacggtc tggtaagcat cattgagcca
     9421 gtcgtccgcc caccgcgtcg tggcgataaa ttcgatcgcg aacaagcgat catcgatgcc
     9481 gccaaagagc tgggcgacag tggcgctgac ctctacaaag ttgaaatgcc gctttatggc
     9541 aaaggtccgc aacaagagct tctctgtgct tcacaacgtc tgaatgacca tatcaatatg
     9601 ccatgggtga tcctctcttc cggtgtcgac gaaaaactgt tcccgcgtgc cgtacgcgtg
     9661 gcaatgacgg cgggcgcatc gggattcctg gcaggccgtg cagtctgggc atcggtcgtc
     9721 ggtttaccag acaacgagct gatgctgcgt gacgtttgcg caccgaaatt acaacaactt
     9781 ggcgatatcg tcgacgaaat gatggctaaa cgccgctaag atgaggataa aaggaatgaa
     9841 atggtttaac accctaagcc acaaccgttg gctggaacag gaaaccgacc gcatctttga
     9901 ttttggtaaa aattcagtag tgccgactgg ttttggctgg ttaggcaata aagggcaaat
     9961 caaagaagag atgggcaccc atctgtggat caccgctcgt atgttacacg tttattccgt
    10021 tgctgcggcg atgggtcgac ctggcgctta ctcgttggtt gatcacggta tcaaagccat
    10081 gaacggcgca ctgcgtgata aaaaatatgg cggctggtat gcctgcgtaa atgacgaggg
    10141 cgtggtggat gcctccaaac agggctatca acacttcttt gctctgctgg gtgccgccag
    10201 cgccgtcaca acgggtcacc cggaagcgcg caagctgctc gattacacca ttgaaattat
    10261 cgagaaatat ttctggagcg aagaagagca gatgtgcctg gaatcctggg acgaagcctt
    10321 cagcaaaacc gaagagtacc gcggcggcaa tgccaatatg cacgcggtgg aagctttctt
    10381 gattgtttat gacgtcactc acgacaaaaa atggctggat cgcgcgattc gcgtggcttc
    10441 cgtgattatc cacgacgtcg ccagaaataa tcattatcgc gttaacgaac acttcgatac
    10501 ccagtggaat ccgctgccgg attacaacaa agataacccg gcgcatcgct tccgcgcgtt
    10561 cggaggtaca ccaggccact ggatcgaatg gggccgttta atgctgcaca tccatgctgc
    10621 cctggaagcc cgttgcgaac aaccaccagc atggctgcta gaagatgcca aaggtctgtt
    10681 taacgccacc gtgcgcgatg cctgggcacc cgatggcgcg gacgggattg tttataccgt
    10741 tgactgggaa ggaaaaccgg tggtccgcga acgtgtacgt tggcctatcg tcgaagcaat
    10801 gggtacggcc tacgcgctct acaccgtcac tggcgatcgc cagtatgaaa cctggtatca
    10861 aacatggtgg gagtactgca ttaagtacct gatggactat gaaaatggtt cctggtggca
    10921 ggagctggat gcggacaata aggtcaccac caaagtctgg gacggcaaac aggatattta
    10981 tcacctgctg cattgcctgg tgatcccgcg tatcccgtta gcgcccggca tggctccagc
    11041 ggttgcagcg ggtctgctgg atattaatgc gaaataactg atctgaaaat agggtgcggg
    11101 ttttcccctc accctaaccc tctccccaaa ggggcgaggg gaccgtccgg tgcagttttt
    11161 gggtttgaca tcagcctcac gggaggctgt atgtcgttaa ttaaggtgcc ttgtatgcaa
    11221 atcaccaata tgcattgcag tggacagacc gtttcacttg ccgcggggga ttatcacgcg
    11281 accattgtga ccgtcggtgc tggtctggct gaattaacct ttcagggttg tcatttagtc
    11341 atcccgcaca aacccgaaga gatgccgctg gcgcatctgg gtaaagtgct tattccgtgg
    11401 ccgaaccgga tcgccaacgg ctgttaccgc tatcaggggc aggaatatca gttacccatt
    11461 aatgaacata gttcaaaagc cgctattcac gggctgcttg cctggcgaga ctggcaaatt
    11521 agtgaattaa ccgccaccag cgttaccctg acagcttttc taccgcccag ttacggctat
    11581 ccgtttatgc tggcttcaca agttgtttat tcgctcaacg cacacacggg tctttccgtt
    11641 gagatcgcaa gccagaatat cggcactgtc gcagcgcctt atggcgtggg tattcatact
    11701 tatttaacct gcaacttaac atctgttgat gaatatctgt tccagcttcc ggcaaaccag
    11761 gtttatgccg ttgatgaaca cgtaaatccg acaacgcttc accacgttga tgagctggat
    11821 ttaaatttca cgcaggcgaa aacaatagcc gcgacaaaaa tcgaccacac ctttaaaacc
    11881 gctaatgatc tgtgggaaat aaccattacc catccacaac aggcgctttc ggttagcctt
    11941 tgttgcgatc aaccctgggt acaaatttat agcggtgaaa aattacagcg tcagggactg
    12001 gcagttgaac ctatgagttg cccgcctaac gcctttaatt caggtatcga cttattatta
    12061 ctggaaccag gaaaaccgca ccgcctgttt tttaacattt acggacagag aaaataactt
    12121 gtattgatta gcagtcaaaa tcattgaaga acatttcact cgctaaaggc aacattatgt
    12181 atcttcaatt tattaagtta aaaacagctc agcttctgta aagtttttat atatcagttt
    12241 tatgagaatc attttacata agcctgtaat aacaggaagg caacaatctc gttctcaact
    12301 ctatggagat tagttatgga tacgccacgt ccacagttat tagattttca atttcatcag
    12361 aataacgaca gttttaccct acattttcaa caacgtctta ttttaaccca tagcaaagat
    12421 aatccttgtt tatggattgg ctcaggtata gcggatatcg atatgttccg cggtaatttc
    12481 agcattaaag ataaactaca ggagaaaatt gcgcttaccg acgccatcgt cagccagtca
    12541 ccggatggtt ggttaattca tttcagccgt ggttctgaca ttagcgccac gctgaatatc
    12601 tctgccgacg atcagggccg tttattgctg gaactacaaa acgacaacct taaccacaac
    12661 cgtatctggc tgcgccttgc cgctcaacca gaggaccata tctacggctg cggcgaacag
    12721 ttttcctact tcgatctgcg tggcaaaccg ttcccgctat ggaccagtga acaaggcgtt
    12781 ggtcgcaaca aacaaaccta tgtcacctgg caggccgact gcaaagaaaa tgcgggcggc
    12841 gactattact ggactttctt cccacagcct acgtttgtca gcacgcagaa gtattactgc
    12901 catgttgata acagttgcta tatgaacttc gactttagtg ccccggaata ccatgaactg
    12961 gcgctgtggg aagacaaagc aacgctgcgt tttgaatgtg ctgacacata catttccctg
    13021 ctggaaaaat taaccgccct gctgggacgc cagccagaac tgcccgactg gatttatgac
    13081 ggagtaacgc tcggcattca gggcgggacg gaagtgtgcc agaagaaact ggacaccatg
    13141 cgtaacgcgg gcgtgaaggt caacggcatc tgggcgcagg actggtccgg tattcgtatg
    13201 acctcttttg gcaaacgcgt gatgtggaac tggaagtgga acagcgaaaa ctacccgcaa
    13261 ctggattcac gcattaagca gtggaatcag gagggcgtgc agttcctggc ctatatcaac
    13321 ccgtatgttg ccagcgataa tgatctctgc gaagaagcgg cacaacacgg ctatctggca
    13381 aaagatgcct ctggcggtga ctatctggtg gagtttggcg agttttacgg cggcgttgtc
    13441 gatctcacta atccagaagc ctacgcctgg ttcaaggaag tgatcaaaaa gaacatgatt
    13501 gaactcggct gcggcggctg gatggctgac ttcggcgagt atctgcccac cgacacttac
    13561 ctgcataacg gcatcagcgc cgaaattatg cataacgcct ggcccgcgct gtgggcgaaa
    13621 tgtaactacg aagcccttga agaaacgggc aagctcggcg agatcctttt ctttatgcgc
    13681 gccggttcta ccggtagcca gaaatactcc accatgatgt gggcgggcga ccagaacgtc
    13741 gactggagtc tcgacgatgg cctggcgtcg gttgtcccgg cggcgctgtc gctggcaatg
    13801 accggacatg gcctgcacca cagcgacatt ggcggttaca ccaccctgtt tgagatgaag
    13861 cgcagcaaag agctgctgct gcgctggtgc gatttcagcg ccttcacgcc gatgatgcgc
    13921 acccacgaag gtaaccgtcc tggcgacaac tggcagtttg acggcgacgc agaaactatc
    13981 gcccattttg cccgtatgac ctccgtcttc accaccctga aaccttacct gaaagaggct
    14041 gtcgcgctga atgcgaagtc cggcctgccg gttatgcgcc cgctgttcct gcattacgaa
    14101 gacgatgcgc acacttacac cctgaaatat cagtacctgt taggtcgcga cattctggtc
    14161 gctccggtgc atgaagaagg ccgtagcgac tggacgctct atctgccgga ggataactgg
    14221 gtccacgcct ggacgggtga agcgttccgg ggcggggaag ttaccgttaa tgcgcccatc
    14281 ggcaagccgc cggtctttta tcgcgccgat agcgaatggg cggcactgtt cgcgtcgtta
    14341 aaaagcatct aatcacatcc gcccgtgggg aaactcatgg gcaaagggag aacatcgatg
    14401 agtcacatca caacggaaga tccagcaact ttacgcctgc cctttaaaga gaaactctct
    14461 tacggtattg gcgacctggc ctctaacatc ctgctggata tcggtacgct ttatcttttg
    14521 aagttttata ccgacgttct ggggctgcca ggcacctatg gcggcattat ctttttgatt
    14581 tcaaaattct ttactgcgtt taccgatatg ggtaccggca ttatgttgga ttcccgacgc
    14641 aagatcggtc caaaaggtaa gttccgtcct tttattctgt atgcgtcatt cccggtcacc
    14701 ttactggcga tcgccaactt tgtcggcaca ccgtttgatg tcaccggtaa aacggtgatg
    14761 gccactattc tgtttatgct ttacggactg tttttcagca tgatgaactg ctcctacggc
    14821 gcaatggttc ccgctatcac caaaaacccc aacgaacgcg cctcactggc ggcatggcgt
    14881 cagggcggcg ctacgctggg cctgctgctg tgcacggtgg gattcgtgcc agtgatgaat
    14941 cttatcgagg gtaatcagca acttggctat atcttcgccg ccacgctgtt ttcactgttc
    15001 ggcctgctgt ttatgtggat ctgctactca ggcgtgaaag agcgttatgt cgaaacccaa
    15061 ccaaccaatc cggcgcaaaa gcctggcctg ttgcaatctt tccgcgcaat tgccggtaac
    15121 cgcccactgt tcattctgtg tattgccaac ctctgcacct taggggcgtt taacgtcaag
    15181 ctcgccattc aggtctatta cacccagtac gtacttaacg atcccatcct gttgtcgtat
    15241 atgggatttt tcagcatggg ctgtattttc atcggcgtgt tcctgatgcc tggcgcagtc
    15301 agacgttttg gtaagaagaa ggtctatatc ggcggcctgc tgatttgggt gctgggcgat
    15361 ctgctcaact atttcttcgg cggcggctcg gtcagctttg tggcgttctc ctgcctggcg
    15421 ttcttcggct cagcgtttgt taacagcctg aactgggcgc tggtttccga caccgtcgag
    15481 tacggcgagt ggcgcaccgg cgtgcgttcg gaaggcacgg tctacaccgg cttcaccttc
    15541 tttcgcaaag tgtctcaggc gctggctggt ttcttccccg gctggatgct gacgcaaatt
    15601 ggctatgtgc cgaacgtcgc acaggctgac cacaccattg aagggttacg ccagttgatc
    15661 ttcatctacc caagcgcact ggcggtagtc accattgtgg cgatgggttg cttctacagc
    15721 ctgaacgaga agatgtatgt ccgcattgtg gaagagatag aagcccgtaa acgcacggcg
    15781 taataataat aaacgacgcc ctgcggggcg ttataaggag tgattatgtc tgaccataat
    15841 ccactgacat taaaactgaa tctgcgggaa aaaatcgcct atggtatggg cgacgtcggt
    15901 tcgaatttaa tgctctgcat cggtactctg tatctcctca aattttatac cgatgaatta
    15961 gggatgcctg cttactatgg cgggattatc tttctggtgg cgaagttttt taccgcgttt
    16021 accgatatgc tcaccggatt tttactcgac tcgcggaaaa atattgggcc taaaggcaaa
    16081 tttcgtccct ttattttata tgcagcagtt ccggcggcgt taattgcaac gctccagttt
    16141 atcgccacca ccttttgttt gccggttaaa acgacgattg ccaccgcgct gtttatgatg
    16201 tttggacttt catacagtct gatgaactgc tcgtatggtg cgatgatccc ggcaattacc
    16261 aaaaacccga atgagcgcgc gcagctcgcg gcttaccgcc aggggggtgc aaccataggg
    16321 ctattgattt gtaccgtggc gtttattccg ttgcagtcgc ttttttctga ctcaaccgtc
    16381 ggttatgcct gtgcggcact tatgttctcc attggcggct ttatttttat gatgctgtgc
    16441 tacagaggcg tcaaagagca ttatgtggac acagcgccaa ccggacataa agccagtatt
    16501 ctcaaatctt tttgcgcgat atttcgtaat ccaccattgc tggttttatg cattgctaac
    16561 ctgtgtactc tggcggcatt taatatcaaa ctggcgattc aggtctatta cacccagtat
    16621 gtgctgaatg atattaattt attgtcgtgg atgggattct tcagtatggg atgcatcctc
    16681 gtcggcgtat tactggtgcc agtaactgta aaatgttttg gtaaaaaaca ggtttattta
    16741 gctggcatgg tgctatgggc ggtgggtgat atactgaatt atttctgggg aagtaactct
    16801 ttcactttcg tcatgttctc ttgtgtcgcc ttttttggca cggcctttgt aaacagcctg
    16861 aactgggcac tggtaccaga taccgtagat tacggtgaat ggaaaaccgg tattcgtgcc
    16921 gaaggttctg tttataccgg ttataccttc tttcgtaaaa tttctgccgc acttgctggc
    16981 ttcttgccag gcattatgct gacgcaaatt ggttatgttc ccaacatcgc gcaaagcgat
    17041 gcgacattac agggtttgcg tcagctcatt tttatctggc cttgtgcact ggcaattatt
    17101 gctgcattga cgatgggatt cttttacaca ctcaatgaaa aacggtttgc attgattatt
    17161 gaggaaatca accaacgcaa aaataaagag atggcgacag aagaaaaaac ggcttccgta
    17221 acgttataaa tgtcaatagc cgctatttcc atcctggtgg gtggcggcct ccctacgttt
    17281 aaaaaatgga cttattatga aaaagattaa tgcaataatt ttattatctt ctctgacctc
    17341 tgcctcggta tttgctggcg catacgtcga aaacagggag gcatataatc ttgcctccga
    17401 tcagggcgag gttatgctac gtgtgggtta taacttcgat atgggcgcgg gtattatgtt
    17461 aaccaatacc tacacctttc agcgagaaga tgaactaaaa catggctata atgaaattga
    17521 aggttggtat cctttattca aaccaaccga taaattaacc atccagcccg gtggcttaat
    17581 taatgataag agtatcggtt cgggtggtgc agtgtatctg gacgtcaact ataaatttgt
    17641 accatggttt aatctgacag tacgaaatcg ctataaccat aataactata gttcaacaga
    17701 tttgagcggg gaactggata ataatgacac ctatgaaatt ggcacctact ggaattttaa
    17761 aatcaccgat aaattttcct atacatttga gccacattat ttcatacgcg taaatgactt
    17821 taatagcagc aatggaaaag atcatcactg ggaaatcact aataccttcc gttatcgcat
    17881 taatgaacac tggctgcctt atttcgaatt acgttggtta gatcgtaatg tcgaaccgta
    17941 ccaccgcgag caaaaccaga ttcgtatcgg gacgaagtat ttcttctgat gtgaaactgc
    18001 cggatgcggc gtaaacgcct tatccggcct acggttgata cggttttcgt tggccggatt
    18061 aagcacatcc ggccattaat caagcttcag ccgccagcat ctctttctta cgttggctaa
    18121 ttcgcttaac cagcattacg gaaaccaccg caccacagaa tgcgaagcag gccataatgc
    18181 caaacacgat tttgtagcca ataatccccg gatttaaatc cagaatgtag ccatacagac
    18241 tgaagcagaa catcgccgga gcgtaaccaa taaagctacc caacgccatc gccgcgcctg
    18301 ttttattttc agcaattttt gcttcgccga taggtgcaaa aaatacggct cgctgtgtaa
    18361 agactatcgc gccaaagccc agcgtacatg ccatccctaa atagaccggc atactttcgt
    18421 gcggcagcat aatcaacagt acgagcgcag cggtactgat gataaaggtg tagcatagat
    18481 atttactcgg cgatttcagg atcttatctg aaatcatacc accaatcggt ccaccaatca
    18541 ttttcagaca gtattggtta atgatgccgt aagcccccac cagcgcaacg ggcaatagat
    18601 agatgttttt caggaatgga atgaagaatg tcaggccaca gtaaaccgcg taaacgaaga
    18661 agacgttaaa agcgataagc cagatggttt tgtctctcag caccgaggtc atactggtgt
    18721 tttgcgatgc tccgtcttct tttttcacct caacggacgg tgcctcttct ttgtcattca
    18781 ggacaaagaa aataataatg cctacggcaa tcacaataag ggaatagaac cagatgcctg
    18841 ctttaaaacc taataagcca ctgccaaacc aggtaaatac tgccaacgca gaaaatgcca
    18901 ccacggtatc gacaatgcca cgccctgttt cgaagaagcc aaacaaccga ccttgttgtt
    18961 cgctattgcc caatcgactt accgacttga gcaagaccgg ccagttcatc atgtcgcaag
    19021 taacgccaaa tagcgcccag acaaagagga tgccccaata ccccggcatt gtcgtcaaat
    19081 aaacacccag taatcctgtc gcaatgagtg agaatgacat ggtgtatctg cgcggtagtt
    19141 tatcggcaaa ataaatagac agaaaaaagc ccactgtggt gacaaatgag tttaccgaca
    19201 tagcattacc aatttgacca ttggtcaaat ggaaatattc ctgcatcggg atataaaacg
    19261 catctttcag cgacggtaat ttataaattg taccgccaca caatgttaat agactaaata
    19321 acgcccattt ctttttcgtg agcataacat cccctaagcg atttttaaaa tcactgtttt
    19381 aaaggcaata agataccgac cattcatatg gaatggtttt attaactgcg tatgagttaa
    19441 aacttattag tggttcagaa gatggtttgc ctcttgtttg atttgttgta gtacgttacg
    19501 cacatgattt atctgattaa ttgcatcttc tttttcttta cgtaaccgcg catccagtaa
    19561 atgattttct ggcaatggtg tttcactcat ctggcgataa gggatccacg gattatcatc
    19621 ttcgtcttca aagcggaacg ccggcgcata gtaatcaacc tcttcttcca ggccatatgc
    19681 cgtcacctgc ggctcatcat cacccaggca tataaggtgc gttaataacg ctttgaacgg
    19741 catatcaccc tgacctgaaa tacaggcttt atgacccagg ccacccggtt ctttaacgat
    19801 caaggcatct ttgatatgga cctgggtaat atgcggcgcc atcgttttta aagcgtcgat
    19861 gggatgctca tttgcattga tcatgttcgc aaaatcaaac agtaaggaaa gagattccat
    19921 ctcactttct ttgaccagcg acaccagctc atggcttttt aaatcttcat gctgttcgat
    19981 cgtaaaagtc aggccgctgt cctgatacgt ttcccgtaca taggcaatgt cgttagcgat
    20041 gatcgataat acgtcgcgca aattaccttc ataacgtgga taaaaacgta cggacgatgc
    20101 cccagttttc aacgcaatgg cgacggcttc gtcaatagat gccttatctg aagcgctggt
    20161 ttcaatatga atatcaaggt tcagacggcg ggctttatcc ccaaaggcag agagttcttt
    20221 atcgtccata ttaccaagag aaaaacgctc gccatccaga acatggattt tgacgccacg
    20281 tagattattt tcacttgcga tatcaagtaa atcagcaggc aacactcttt ccagccgcat
    20341 atttaaatga aaggcataag catgtaaata aagaggaaga gtgtcgacac gttgtagaat
    20401 ctttcttgcg ttatttattg tcaccataaa acacctacag attaatgatg aggtctacgg
    20461 cctcaaatcg ccaatattca atatctatct ggacaatatt gccttgctgg tcggcgcgat
    20521 gcttttcaat taagatcgct ggcatacccg cagaaccgcc aagatacttg ctgatatcgc
    20581 ccggcattct gacgggtttg aaaatcaatt tttttactac cgtctctttt tggtagcact
    20641 tttcccagac tgcagaaaat gagtggtttt caagttgttc aataaaaccc ggagcaactt
    20701 caggattaat atatgtttcg tgatagaaaa ctttatgtcc ttccagcgcc ccccagccgg
    20761 tgatgcgata gagttcagtt gatggtgtca ctgcaatcag tggcgcgagc gtcttgggaa
    20821 tatcgctggt acggtttttc tcggtaaacc cccaggaagg ctctcgtcct tgctcaattg
    20881 cggcccgctg aaagctcgcc gacaactccg gactgtaatt aaatcgcggc tgggtcacga
    20941 accacccctt acgatcctta cggaagattt tggattctgc ctcgaggttc aacaacgcct
    21001 ggcgcagcgt catacgttta atgcccagca gttctcccag ctctctttct gaagggagtt
    21061 ttccacccgg cgtggtgatg ccatctttca accaccgatc cagtttctct tttgcatttt
    21121 ctacggtgga ttgattctcc gccattgttt aatccttttg gtttaaacca gatgaagcat
    21181 cttacgctta cgccaaaaaa gaaagcaatt aacggcgtat gactttgcag ataattgtga
    21241 caaagctctc tattgacgta atcccagact tataagggag atactcgcat tggtgtgcgc
    21301 tcacgacagg taaaaaaaaa cctgccagcg atggcaggtt ttttataact aaaaaacgaa
    21361 attaatcgtc tttcggtgcg cggttggcgc ggcgacgatc gttttccgtc aggtgacgtt
    21421 tacgaatacg gatagaggtc ggagtcactt ctaccagttc gtcatcatcg atgaactcca
    21481 gagcttgttc cagagtcatg cggataggcg gaaccagaac aacggcttcg tcagtaccgg
    21541 aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt taccgtcagg tcgttagagc
    21601 ggctatgaat accgataatc tgaccttcgt aaacttctgc accgtgaccg aggaacagct
    21661 taccgcgatc ctgcagaccg aacagcgcga acgcgaccgc tttaccctga ccgttagaga
    21721 tcagtacgcc gttctgacgc tgacccactt cacccggacg tacgtcgtcg tagtggctga
    21781 aggtggagta cagcagacca gtaccggaag tcatggtcat gaactcagaa cggaagccaa
    21841 tcagaccacg gcttgggatc acgtagtcga gacgtacgcg gcctttaccg tctggattca
    21901 tgtttttcag gtcgccttta cgttcgccca gcgcctgcat tacagaaccc tgatgctgtt
    21961 cttcaacgtc cagagtcacg ttttcatacg gctcttgttt acgaccgtcg atttcacgga
    22021 agataacttt cggacgggat accgccagtt cgaaaccttc acgacgcatg ttttcgatca
    22081 gaacagacag gtgcagttcg ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt
    22141 cttctacgcg cagcgcaacg ttgtgtacca gttctttgtt cagacgatcc aggatctgac
    22201 gagacgttac gaacttacct tctttaccgc agaacggcga ggtgttaacg cagaagaaca
    22261 tagaaacggt cggctcatca acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc
    22321 aaacggtgtc agaaatgttc agttcgccaa ggcccgtgat cgcaacgata tcgccagctt
    22381 ccgccagatc ggtttcgata cgttccagac cgaggtggcc cagcacttta ccgactttcg
    22441 cgttgcgggt tttgccttcg ctatcgatga tagtgacctg ctggttcggc ttcactttac
    22501 cgcgcttgat gcggccaatg ccgataacgc caacatagct gttgtaatcg agctgagaaa
    22561 tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg
    22621 cctggtacag cggggtcatg tcttccgcca tatcttcgtg gtccagaccc gcgataccgt
    22681 tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa
    22741 cgaacagatc gaatacctga tccacaaccc aatcaggacg cgcgccaggg cggtcaactt
    22801 tgttgataac aacaatcggc ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg
    22861 tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag cactgagtct accatggaca
    22921 ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac
    22981 ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat ggtaatcccc cgctctttct
    23041 ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc acgagagtcg aacgtaccgg
    23101 attgttggag cagcttgtct accagggtgg ttttaccatg gtctacgtgc gcgatgatgg
    23161 cgatattacg caatttttcg atcacaactt tgcctcaggc attagaaata gcgcgttatt
    23221 gtacacggat taatcgcact acaaaacagg atcacaaaca tcctccgcaa acaagtattg
    23281 cagagtccct ttgtgatcgc tttcacggag cataaaaagg gttatccaaa ggtcattgca
    23341 ccaacatggt gcttaatgtt tccattgaag cactatattg gtgcaacatt cacatcgtgg
    23401 tgcagccctt ttgcacgatg gtgcgcatga taacgccttt taggggcaat ttaaaagttg
    23461 gcacagattt cgctttatct tttttacggc gacacggcca aaataattgc agatttcgtt
    23521 accacgacga ccatgaccaa tccaggagag ttaaagtatg tccgctgaac acgtactgac
    23581 gatgctgaac gagcacgaag tgaagtttgt tgatttgcgc ttcaccgata ctaaaggtaa
    23641 agaacagcac gtcactatcc ctgctcatca ggtgaatgct gaattcttcg aagaaggcaa
    23701 aatgtttgac ggctcctcga ttggcggctg gaaaggcatt aacgagtccg acatggtgct
    23761 gatgccagac gcatccaccg cagtgattga cccgttcttc gccgactcca ccctgattat
    23821 ccgttgcgac atccttgaac ctggcaccct gcaaggctat gaccgtgacc cgcgctccat
    23881 tgcgaagcgc gccgaagatt acctgcgttc cactggcatt gccgacaccg tactgttcgg
    23941 gccagaacct gaattcttcc tgttcgatga catccgtttc ggatcatcta tctccggttc
    24001 ccacgttgct atcgacgata tcgaaggcgc atggaactcc tccacccaat acgaaggtgg
    24061 taacaaaggt caccgtccgg cagtgaaagg cggttacttc ccggttccac cggtagactc
    24121 ggctcaggat attcgttctg aaatgtgtct ggtgatggaa cagatgggtc tggtggttga
    24181 agcccatcac cacgaagtag cgactgctgg tcagaacgaa gtggctaccc gcttcaatac
    24241 catgaccaaa aaagctgacg aaattcagat ctacaaatat gttgtgcaca acgtagcgca
    24301 ccgcttcggt aaaaccgcga cctttatgcc aaaaccgatg ttcggtgata acggctccgg
    24361 tatgcactgc cacatgtctc tgtctaaaaa cggcgttaac ctgttcgcag gcgacaaata
    24421 cgcaggtctg tctgagcagg cgctgtacta cattggcggc gtaatcaaac acgctaaagc
    24481 gattaacgcc ctggcaaacc cgaccaccaa ctcttataag cgtctggtcc cgggctatga
    24541 agcaccggta atgctggctt actctgcgcg taaccgttct gcgtctatcc gtattccggt
    24601 ggtttcttct ccgaaagcac gtcgtatcga agtacgtttc ccggatccgg cagctaaccc
    24661 gtacctgtgc tttgctgccc tgctgatggc cggtcttgat ggtatcaaga acaagatcca
    24721 tccgggcgaa gccatggaca aaaacctgta tgacctgccg ccagaagaag cgaaagagat
    24781 cccacaggtt gcaggctctc tggaagaagc actgaacgaa ctggatctgg accgcgagtt
    24841 cctgaaagcc ggtggcgtgt tcactgacga agcaattgat gcgtacatcg ctctgcgtcg
    24901 cgaagaagat gaccgcgtgc gtatgactcc gcatccggta gagtttgagc tgtactacag
    24961 cgtctaatag ttgaagttgt actacccggc gcaacaacgc cggaggttca aaccaggccc
    25021 atctggcgta attgttgcag ccagtttgaa cacggacagc gcgcagaacc cggagcgtac
    25081 actggtacgt gaggagttcg agcactgccc aggttcaaaa tggcaaataa aatagcctga
    25141 tgggactggt ttttagttgc cgtggaaatt tcagcccatc tctgcatggg cttttttctc
    25201 cgtcaattct ctgatgcttc gcgcttttta tccgtaaaaa gctataatgc actaaaatgg
    25261 tgcaacctgt tcaggagact gctttatggc aacaggcacg cagcccgatg ctgggcagat
    25321 cctcaactcg ctgattaaca gtattttgtt aatcgatgac aacctggcga tccattacgc
    25381 caaccctgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg gtacaccgtt
    25441 accggaactg ttgagctact tctcattaaa tatcgagctg atgcaagaaa gtctggaggc
    25501 ggggcaaggt tttaccgata acgaagtgac gctggtcatc gacgggcgct cgcatatcct
    25561 ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg ctccgatgga
    25621 taaccagcgc cgcttaagtc aggaacagct acagcacgcc cagcaggttg ctgcccgtga
    25681 tttagtgcgc ggcctggcac atgagattaa aaatccgctt ggcggtttac gtggcgcggc
    25741 gcagctgctc agcaaagcgt tacctgaccc atcactactc gaatatacca aagtgattat
    25801 cgaacaggcg gaccggctgc gaaatctggt cgaccgtctg ttggggccgc agctgcccgg
    25861 tacgcgcgtt accgaaagta ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat
    25921 ggaactgccg gacaacgtgc ggttgattcg tgattacgat cccagcctgc cggaactggc
    25981 gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc
    26041 gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc aactgacctt
    26101 acacggcgag cgctaccggc tggcggcgcg gattgatgtg gaagataacg ggccgggcat
    26161 tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg aaggtggcac
    26221 cgggcttggc ttatccatcg ctcgtaattt gattgatcag cattcaggca aaattgaatt
    26281 taccagttgg ccagggcata ccgagttctc ggtttacctg cctatcagga aataaaggtg
    26341 acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat ccgttgggtg
    26401 cttgaacgtg cgctcgctgg ggcaggttta acctgtacga cgtttgagaa cggcgcagaa
    26461 gtgctggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat ccgtatgccg
    26521 ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat gcttccggtc
    26581 atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca acaaggggcg
    26641 tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt tgagcgcgct
    26701 atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg cccaacgacc
    26761 gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg tcggctttcg
    26821 cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga actggtcgct
    26881 catgccctgc atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct gaatatggca
    26941 gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact
    27001 ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggtac attattcctc
    27061 gacgaaattg gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac
    27121 ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg gattatcgct
    27181 gccactcacc agaatctcga acagcgagtg caggaaggta agttccgtga ggatctgttc
    27241 caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt
    27301 ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag
    27361 ttactgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg caacgtgcgc
    27421 cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga agtgttgatt
    27481 caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc gcaaatgcaa
    27541 ccggacagct gggcgacgct tcttgcgcag tgggcagaca gagcgctgcg ttccggtcat
    27601 caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac gaccgcgttg
    27661 cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg ccgcaacacc
    27721 ctgacgcgta agttaaaaga gctggggatg gagtgattca cagcttgtgt gtaaagattg
    27781 attattgagc gcaaattgct ggtattttac gctttactgt tccgataagt tcagtatgat
    27841 cttgcccgga aaacggggag agtcattatg ctggaatcaa taattaatct ggtatcgagt
    27901 ggcgcagttg acagccacac accgcaaact gccgttgctg ccgtgctgtg tgccgcaatg
    27961 attgggctgt ttagttgagt ttcagattgc tgatgaagtg ctctttgttc atgccggatg
    28021 cggcgtgaac gccttatccg gcctacaaaa gcaatatttt caacaggttt tgtaggcctg
    28081 ataagcgtag cgcatcaggc aatcttgttc tcagcacatt gatggcgctt tcgttttcta
    28141 ctttgtaaac gaagcgccat tcactattta aatcacccgt gagaactgct gcatccgcgc
    28201 tttctggcgc agataggtat caaagcacat gcaaatgttg cggatcagca agcgaccttt
    28261 cgccgtcacc tgtattccct tctcatccac atccaccagc ccatcttttg ctaacggggc
    28321 gagcagcttg agatcttccg caaagtaatc agcgaagtgc aaatcccaca gtttctcaat
    28381 aggggcgtaa tccagacgga agttgcagat gagcgactta atcacatcgc ggcgaataca
    28441 gtcatcacgc gttagcgcaa taccacgcca cagcgcattg ccttgttcat ccacttgctg
    28501 atagtactgc ttcaactctt tctggttctg cgcgtagcag tcgccaatca tgctgatggc
    28561 ggaaacgccc atccccagca gatcggtatc gccctgagtg gtgtagccct ggaagttacg
    28621 atgcagcacg ccttcacgct gggccaccgc cagctcgtca tccggacggg caaagtgatc
    28681 cataccgata aactgatagc ccgattgcgt caggaaggcg atggtttcct gcaggatatc
    28741 gagtttttgc tgcggactcg gcaggtcagc atctttgatt ttgcgctgag cagcaaaaat
    28801 ggtcggcaga tgcgcgtagt taaagacact cagacgatcg gggttcagct ccgccacacg
    28861 tttcagggta aaggcgaaac tctccggcgt ctgtttcggc aggccgtaaa tcaggtcgat
    28921 gttggtggag gtaaaaccaa tctcacgcgc atggttaagc agtgcaaaga tgaactcttc
    28981 atcctgctcg cggttaacca gacgttgcac ttctttgttg aagtcctgca cgcccatgct
    29041 caggcgatta aagccttcgg cgcgtaaatg atcgagtaca tccagttcga tttcccgcgg
    29101 atcgacttcg atcgaaatct ccgcatcggc attgaactgg aagttttcgc gcagcagctt
    29161 catcaggcgg ctgatttgcg ctttattcag atacgtcggc gttccgccgc cccagtgcaa
    29221 ttggctgacg tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt gctccagcgc
    29281 gtccagatac tgatcggcct tgtgctgctg gcgagtaaca atcttattgc aaccgcagaa
    29341 gtaacaaagc ttatggcaga acgggatatg tacgtagaga gataatggac gctcaggata
    29401 gcgcgccacg gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact ccagcgcggt
    29461 cgggtacgag gtgtatcgtg gcccggaata gttatatttc tggatcaggg ccagatccca
    29521 gtcgatttgc tgtacagaca tgctcactcc ttccgatggc gtctctggcg gctacggcgt
    29581 accggcggat gcccgtttcg ggtcatcagc cggttgcgca gccacttttg tcgccgcgac
    29641 aaccgctgta gtttaacgaa taaccacacc agataacata taaccggaag ggttatcagc
    29701 agaacgggaa ggcccacggc ggcaatccgt tagttgcccc gcagcagacg catcatgtct
    29761 tcttgcttct cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc gagtttctgc
    29821 atcaactcat caatacgatc cagtttagca tccacccagg attgctcttc ggcgctcagg
    29881 gtttcgcctg cttccagacg ttccagcagc gcatccagac gctcatccgt ttccagtaat
    29941 tccaactccg cctgcggtga aagcataggt ttctcactct tcggtttgtg ctgtttggtg
    30001 actttttcag tcacgcccaa tggaataggg gttttactgc caatacgtgg atcttttggt
    30061 gcgttctggc ctttgctgcc tgacgtggtg tttccgcccg ctgcgcggct gcccggcgca
    30121 tgaccacgac gttttttctg acgcttgcga tcacgagctt cctgatccag ctcctcgcgt
    30181 gtttttcgac gcgcttttgc atgacctttg ctgcgtgagt ttgaagatga tggtttcata
    30241 atggttcgtc ttttagccgt tttattcagt atagatttgc ggcggaatct aacagaaagc
    30301 aagcaaagaa aaaaggcgac agattactct gtcgcctttt ttcctgactc ataaccctta
    30361 acgggtctta caatccttgc aggccgccaa cacaccctgt ttttccttta gccggaacaa
    30421 tccgtgttga ttccatttcc gtttaattac gtctccggac gtttgtcttc ctgacaatcc
    30481 tctgtcttcg cctcctggcg ctcctgaccc tcatcctgag tcattgttcc tgttagcgtc
    30541 ctcgctctat gggtgctact ttacgccttt gctttaaaca aacaatcaac cgccatcagt
    30601 atttgcacat tttcagagtt ttatataaaa gcaattcact gatttatata gcttatttga
    30661 catgcatcgc agaattttct ctgctatttc gcataaagac tatcggcaat gtctcacaaa
    30721 gcgtatggct tttacttaca acgctcacgc cgaaaaatcc gccttttatt atggttcagg
    30781 tataatcgcc acaaagcttt gactgacgga gacgaccgct ttgactaatt tgaattatca
    30841 acagacgcat tttgtgatga gtgcgcctga tattcgccac ctaccttccg ataccggaat
    30901 tgaagtggct tttgcaggcc gttccaacgc aggtaaatcc agcgcgctga acacgctgac
    30961 taaccagaaa agcctggctc gtacctcaaa aaccccaggg cgcacccagc ttatcaacct
    31021 gtttgaagtg gctgacggca agcgtctggt tgacttgcct gggtacggtt atgcggaagt
    31081 cccggaagag atgaagcgca aatggcagcg tgcgctcggc gaatacctcg aaaaacgtca
    31141 gagcctgcaa ggtctggtgg tgctaatgga tattcgccat ccgctgaaag atttggatca
    31201 gcagatgatt gagtgggcgg tagacagcaa tatcgccgtt ctggtgctgc tgaccaaagc
    31261 ggacaaactg gcaagcggcg cacgtaaagc gcaattgaat atggtgcgtg aagctgtact
    31321 ggcgtttaac ggtgatgtgc aggttgaaac gttttcttcg ttgaagaaac aaggcgtgga
    31381 caagctgcgg cagaaactgg atacctggtt tagcgagatg cagcctgtag aagaaacgca
    31441 ggacggcgaa taattttctt gccttaatgc ttgtgccgga tgtggcgtat ccggcccgta
    31501 aattcaatag attacaattc taccgttggc ctgataaaca tagcgcatca ggcattacgg
    31561 atcttttctt tcgcccaata aaaaacgccc cagtcattac tgactggggc ggctaaaata
    31621 ttcagccaaa tccgattacg tgaagtaaaa ggtctgaaag atagaacatc ttacctctgt
    31681 accctacgcg actaacttta ctcttttttg ttcagttcag aaagcacttt ttgtaatttt
    31741 ttttcagttg ttgcatagga atttctaata gccatcacaa aacgtgacag cttatgttgc
    31801 ttacttacga aaaaagggca tgttcaggcg aatcttagtg cgcctgatcc cagttttcgc
    31861 cactccccac ttccaccagc aacggcacat ccagacgggt acagttttcc atcagttgat
    31921 gaatctgctt cgcgacggca tcaacatcat ctttatgaac ttcaaatacc agttcatcgt
    31981 gtacctgcat gatcatacgt acacgcggtt gctcagcctg taaccacgca tcaacggcaa
    32041 tcatcgcccg tttgataatg tcggcggcgg ttccctgcat tggcgcgtta atggctgcac
    32101 gttcagccgc tgcacgacga gcaccattgc tggatttgat atccggcaga tacagacggc
    32161 gtccgtccag cgtttcaacg tagccctgct ctttcgcctg agcacgggtg cgttccatat
    32221 actccagcac gccagggtag cgttcgaagt aaaggtccat gtacttctgc gcttctttac
    32281 gtggaatgtt caattgccgc gccagaccga aagcactcat gccataaatc agaccaaagt
    32341 tgatcgcttt cgcgctacgg cgttgctcgc tggtgacggt ttccagtggc aaactaaaca
    32401 cttctgccgc cgttgcccgg tggatatctt ttccttccgc gaatgcggtc agcaagcctt
    32461 tgtcacgcga aagatgcgcc ataatgcgca gttcaatctg cgagtagtcc gctgagacaa
    32521 tcacataatc ctctggcgca ataaacgcct ggcggatacg acgaccttct tcgttacgca
    32581 ccggaatgtt ttgcaggtta ggatcggttg acgataaacg tcccgttgca gttactgcct
    32641 ggtgataaga ggtatgcaca cgcccggttt tcgggttgat catcagcggc agcttgtcgg
    32701 tgtaggtcga tttcagcttc gccagaccac gatactccag aatcactttt ggcaacggat
    32761 agtccagcgc cagttcttcc agtacctctt ccgacgttga cggcgcgcca cccggcgttt
    32821 tcttcagcgg tttaataccc tgtttttcaa agagaatggt ttgcaactgc ttggtggaag
    32881 aaaggttaaa ttcttcacct gcaatttcat gcgctttctt ttccagctca gccagacgca
    32941 aggtgagctc ttcagaatga ttgtgcagca ctttcggatc gatcttcaca ccgttacgtt
    33001 caatgcgtga aagcaccggc accagcggca tttcgatatt ctcgaagacg ttcaacggcc
    33061 ctttgtgttt ttgcagatct ggccacattt tcagatgcaa ctgcaaggtg acatcagcat
    33121 cttcggcggc gtaacgtccg gcctcttcga gggcaatctg gttaaaggtc agttgatttt
    33181 tgcctttacc cgcaatctct tcaaaagtga tggttttgtg cttcaaccaa cgttccgcga
    33241 ggctgtccat atcgtgacgc ccggcaacgc tattgagaat gtaggactcc agcatggtat
    33301 caaacgcaat cccacgcagc tcaatgccgt aattcgccag aataccgcga tcgtatttca
    33361 ggttttgccc gaccttcagc gccttttcat cttccagcag cggttttagc aactcgagtg
    33421 cacgctcgcg agagatttga tcgggcgcgt caagataatc atgagcaacc ggaatatatg
    33481 ccgctacgcc tggctcgata gcaaaagaaa gcccgaccag gttagcagag atgttatcaa
    33541 ggctgtcggt ttcggtatca aatgcaaata ccggcgcttt ttccagcttc gcaatccacg
    33601 ctttcagtgt ttcttcatca aggatggtga cgtagttgtc ataagaaatc accgttgccg
    33661 tcacttctgg cgcttcgtct gcaacactgg tttcctgcgg cttcgcggct ggttttgccc
    33721 ctttggcctg taaccatttg cccgcttcga catcagcagt ccagcgtttg aactcatact
    33781 ttttgaacag ccccaacaac tcttccgctg ccggttgctg cacttccagt tgttcacagg
    33841 tcagctccag ttcaacgtcg gttttaatcg tcgccagctg gtatgagaga taagcaactt
    33901 ctttgttttg ctcgagcttc gctgccattg ttttcgcgcc acggaagctc aacccagcaa
    33961 ttttttctgg ctcggcatac agcgtatcca gcccgccaag accttgcagc aatgcctgcg
    34021 cggttttttc accgacgccc ggtacgccag gaatgttatc ggaggagtca cccatcagcg
    34081 ccaggaaatc gatgatcagt tctggcggca cgccgtactt attcaccacc tcttccggtc
    34141 cgaggatggt attcgtcatg gtattgataa gcgtaatatt tggcgtcacc agctgcgcca
    34201 tatctttatc gccagtgctg atcagcaccg gacgcccggc tttttcggct tcgcgcgcca
    34261 gagtaccgat aacgtcgtcc gcttctacgc cagaaaccgc cagcagcggc agtcccatcg
    34321 ctttaaccat cgcgtgcagg ggttcgattt gtgctcgcag atcgttcggc attggcgggc
    34381 gatgtgattt gtaatgttca aacagttcat cacgaaaggt ttttcccttg gcgtcaaaga
    34441 ccaccgctgc atgcgtcggt ttatattgca tgatcagact gcgcagcatg ttgaggacac
    34501 catacatcgc accggtcggc tcgcctgcgc tgttagtcag cgggggaaac gcgtgatatg
    34561 cgcgataaag ataagatgaa ccatctacaa ggataagtgg attttggggg atctgaacca
    34621 taatgtccgt gcctgtttat cagattatgg gtaaaggatg ccacagacag gatgaaaaca
    34681 tgagtttttc accgcttttc gtgaaaatct tttgtagatc ttctggatcg ctcgcaaatc
    34741 gtcatgtgga taactttgtg cataaattat ttacatcgct tttaatttat tatcatgctt
    34801 tttacagcat gatatttgtc tttgtttttc atgcagttaa aaataaagcc atccctaata
    34861 attgagataa tgggattttt tctaatgtgg atagatatga attatttttc tccttaagga
    34921 tcatccgtta tttgggtcgt tttctgctaa aatctgcgcc ttgcgcttct ttagcgcact
    34981 gtctatggct aaccttttga ataaattcat tatgacgaga atactcgctg cgataaccct
    35041 tttgctgagt atcgtattga ccattttggt cactattttt tgttccgtcc cgatcatcat
    35101 tgccgggatt gtaaaactat tgctacctgt gccagtcatt tggcgaaagg tttcgcgttt
    35161 ttgtgatttt atgatgtatt gctggtgtga aggtctggcg gtattactgc atctgaaccc
    35221 acacctgcaa tgggaagttc acgggctgga agggttaagt aagaagaact ggtatctgct
    35281 tatttgtaac caccgtagtt gggcagatat tgtcgtactg tgcgtgctgt ttcgtaagca
    35341 cattccgatg aataaatatt tcctcaagca acagctggcc tgggtgccat ttcttggcct
    35401 ggcgtgctgg gcgctggata tgccgtttat gaagcgttat tcccgcgctt atttattacg
    35461 tcatccggag cgacgtggta aagatgttga aaccactcgg cgttcttgtg aaaagtttcg
    35521 cctgcatccc accaccattg tcaatttcgt tgaaggctcc cgtttcacgc aagaaaaaca
    35581 tcagcaaacc cactccactt ttcaaaactt gttgccacca aaggcggcag gcattgcgat
    35641 ggcgctaaat gtactgggta aacaattcga taaactgctt aatgttacgc tgtgttatcc
    35701 ggacaataac cgtcagccgt tcttcgatat gttaagcggt aaactgacgc gaattgtcgt
    35761 tcatgtggat ttacagccta ttgccgatga gttacatggc gactacatca atgacaaaag
    35821 ctttaagcgc cattttcagc aatggctgaa ctcactgtgg caggaaaaag accaacttct
    35881 gacgtcgttg atgtcatcac agcggcagga caagtaaatt ttgctcgcct ctgaatgagc
    35941 agaggcgagt gagtatttta atgaacaaaa cgtccggcac gagacataaa ttcttcttcg
    36001 ctcatctcct gtccgttaaa aacaacttta cccggtgtat aacgcagttg cagcgaggcg
    36061 gtattgttgt caatcgtgat taactgaaac atctgaccta aggtcatcat cccgctgatt
    36121 tgtttatcag cccgcttttg cgctttttcc gcatccattc cttcagataa attaagctgt
    36181 tttgcggttt ctgtgacgac attaagcggt aacttcacat caaaattgag cgatttaata
    36241 tctttgtttg tggatgatga agatttggct gggtcggcaa tactgatatc cagattggca
    36301 tttagttcgc cgagtgcgtt cttccatttt accggttgtt taatggtcgg ctcacttttt
    36361 tgtaataacg gtaaatactc tttgaacaat gcagcattca cttcttgcag agcaacttca
    36421 tcgtttgcta actcagggtg tgcagcaagc tgcttctgca ttgcaatgtt atactggatg
    36481 ataaattggc gcaccgctga gggatcaata gattcagcaa tcaatgacca ctcaccacta
    36541 ccgagttgct gattcccttt tgttaactta tcaagttcgt aggtcagatt gacattgata
    36601 taatctttgt ctttaacgcg atccagcatg gtttttgcaa cgaaagcatc gatttgggca
    36661 acgtcttccc cgtgattgat aatgttaatc tgattaaatt tcgactcgct ttcaccaacc
    36721 ggaaatttct tttcttctaa tcgcgccaga gaatcaatgg taaaactttt tgccgttact
    36781 ttgttcattg gtgataattg cagaacaata tcttcgactt tgccttcaat atccaggtta
    36841 gacaatgaac taccatcacc attaaaagta aactggccct caccaaaaga aaactttcca
    36901 tactctgcag gggcaacatc taatgtcgta gagaatgagc caccaaacgc gaatcgcaat
    36961 gtcgcttcca cgggcgattt atttttcgcg gcgataaata gcggttgtgt cagttcatta
    37021 ttcactaagt tcacttttgc cgctgccagt gctgggatga cattaccatg cattaacatt
    37081 gtgatgggca acggaccgtg ctcaacatcc acatcaaata caactttctg gcctggcttg
    37141 atattgagat cgggtgcacc gttatcgaat gtcatttgca tttgaaaacg ggaattaaaa
    37201 aagcctttgt caaagttttt ataacttaac gtcatatcgt aggcatgttc acctttcttt
    37261 ttcgcatcgt taaaatcttt aataaacttt tcgataccag gctgaatttg cgtacctgtg
    37321 taccatgtgc caccacccca gattacggct aacgcaacaa taacacctgt agctgactta
    37381 cgtatcatat aaatattccc tgataaaaca gcaaaacttt gaatatccac ttatgctgaa
    37441 ataatgtcca gcggcaggat gc
//
LOCUS       DAZFKM010000033        36964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFKM010000033 DAZFKM010000000
VERSION     DAZFKM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..794)
                     /gene="rlmB"
                     /locus_tag="ACHKAY_001796"
     CDS             complement(63..794)
                     /gene="rlmB"
                     /locus_tag="ACHKAY_001796"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO5700483.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(974..3415)
                     /gene="rnr"
                     /locus_tag="ACHKAY_001797"
     CDS             complement(974..3415)
                     /gene="rnr"
                     /locus_tag="ACHKAY_001797"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO5700484.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3454..3879)
                     /gene="nsrR"
                     /locus_tag="ACHKAY_001798"
     CDS             complement(3454..3879)
                     /gene="nsrR"
                     /locus_tag="ACHKAY_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO5700485.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4084..5382)
                     /gene="purA"
                     /locus_tag="ACHKAY_001799"
     CDS             complement(4084..5382)
                     /gene="purA"
                     /locus_tag="ACHKAY_001799"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO5700486.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5486..5683)
                     /gene="yjeT"
                     /locus_tag="ACHKAY_001800"
     CDS             complement(5486..5683)
                     /gene="yjeT"
                     /locus_tag="ACHKAY_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO5700487.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5765..6769)
                     /gene="hflC"
                     /locus_tag="ACHKAY_001801"
     CDS             complement(5765..6769)
                     /gene="hflC"
                     /locus_tag="ACHKAY_001801"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO5700488.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6772..8031)
                     /gene="hflK"
                     /locus_tag="ACHKAY_001802"
     CDS             complement(6772..8031)
                     /gene="hflK"
                     /locus_tag="ACHKAY_001802"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO5700489.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8117..9397)
                     /gene="hflX"
                     /locus_tag="ACHKAY_001803"
     CDS             complement(8117..9397)
                     /gene="hflX"
                     /locus_tag="ACHKAY_001803"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO5700490.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9474..9782)
                     /gene="hfq"
                     /locus_tag="ACHKAY_001804"
     CDS             complement(9474..9782)
                     /gene="hfq"
                     /locus_tag="ACHKAY_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO5700491.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9868..10818)
                     /gene="miaA"
                     /locus_tag="ACHKAY_001805"
     CDS             complement(9868..10818)
                     /gene="miaA"
                     /locus_tag="ACHKAY_001805"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO5700492.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10811..12658)
                     /gene="mutL"
                     /locus_tag="ACHKAY_001806"
     CDS             complement(10811..12658)
                     /gene="mutL"
                     /locus_tag="ACHKAY_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO5700493.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12668..14005)
                     /gene="amiB"
                     /locus_tag="ACHKAY_001807"
     CDS             complement(12668..14005)
                     /gene="amiB"
                     /locus_tag="ACHKAY_001807"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO5700494.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14024..14485)
                     /gene="tsaE"
                     /locus_tag="ACHKAY_001808"
     CDS             complement(14024..14485)
                     /gene="tsaE"
                     /locus_tag="ACHKAY_001808"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO5700495.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14457..16004)
                     /gene="nnr"
                     /locus_tag="ACHKAY_001809"
     CDS             complement(14457..16004)
                     /gene="nnr"
                     /locus_tag="ACHKAY_001809"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO5700496.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16003..17142
                     /gene="queG"
                     /locus_tag="ACHKAY_001810"
     CDS             16003..17142
                     /gene="queG"
                     /locus_tag="ACHKAY_001810"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO5700497.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17125..17178)
                     /locus_tag="ACHKAY_001811"
     CDS             complement(17125..17178)
                     /locus_tag="ACHKAY_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700498.1"
                     /translation="MRFYFYSQAVDESGVTW"
     gene            complement(17412..17487)
                     /locus_tag="ACHKAY_001812"
     tRNA            complement(17412..17487)
                     /locus_tag="ACHKAY_001812"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17452..17454),aa:Gly,seq:gcc)
     gene            complement(17523..17598)
                     /locus_tag="ACHKAY_001813"
     tRNA            complement(17523..17598)
                     /locus_tag="ACHKAY_001813"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17563..17565),aa:Gly,seq:gcc)
     gene            complement(17756..17831)
                     /locus_tag="ACHKAY_001814"
     tRNA            complement(17756..17831)
                     /locus_tag="ACHKAY_001814"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17796..17798),aa:Gly,seq:gcc)
     gene            complement(18042..18587)
                     /gene="orn"
                     /locus_tag="ACHKAY_001815"
     CDS             complement(18042..18587)
                     /gene="orn"
                     /locus_tag="ACHKAY_001815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5700499.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18682..19734
                     /gene="rsgA"
                     /locus_tag="ACHKAY_001816"
     CDS             18682..19734
                     /gene="rsgA"
                     /locus_tag="ACHKAY_001816"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5700500.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19831..20799
                     /gene="asd"
                     /locus_tag="ACHKAY_001817"
     CDS             19831..20799
                     /gene="asd"
                     /locus_tag="ACHKAY_001817"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5700501.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20821..24144
                     /gene="mscM"
                     /locus_tag="ACHKAY_001818"
     CDS             20821..24144
                     /gene="mscM"
                     /locus_tag="ACHKAY_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5700502.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24173..24487)
                     /gene="yjeO"
                     /locus_tag="ACHKAY_001819"
     CDS             complement(24173..24487)
                     /gene="yjeO"
                     /locus_tag="ACHKAY_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5700503.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24484..24798)
                     /gene="yjeN"
                     /locus_tag="ACHKAY_001820"
     CDS             complement(24484..24798)
                     /gene="yjeN"
                     /locus_tag="ACHKAY_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5700504.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24850..26352)
                     /gene="yjeM"
                     /locus_tag="ACHKAY_001821"
     CDS             complement(24850..26352)
                     /gene="yjeM"
                     /locus_tag="ACHKAY_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5700505.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26571..27548)
                     /gene="epmA"
                     /locus_tag="ACHKAY_001822"
     CDS             complement(26571..27548)
                     /gene="epmA"
                     /locus_tag="ACHKAY_001822"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5700506.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27873..29681
                     /gene="frdA"
                     /locus_tag="ACHKAY_001823"
     CDS             27873..29681
                     /gene="frdA"
                     /locus_tag="ACHKAY_001823"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5700507.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29674..30408
                     /gene="frdB"
                     /locus_tag="ACHKAY_001824"
     CDS             29674..30408
                     /gene="frdB"
                     /locus_tag="ACHKAY_001824"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5700508.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30419..30814
                     /gene="frdC"
                     /locus_tag="ACHKAY_001825"
     CDS             30419..30814
                     /gene="frdC"
                     /locus_tag="ACHKAY_001825"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5700509.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30825..31184
                     /gene="frdD"
                     /locus_tag="ACHKAY_001826"
     CDS             30825..31184
                     /gene="frdD"
                     /locus_tag="ACHKAY_001826"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5700510.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            31247..32380
                     /gene="ampC"
                     /locus_tag="ACHKAY_001827"
     CDS             31247..32380
                     /gene="ampC"
                     /locus_tag="ACHKAY_001827"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5700511.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32469..33002
                     /gene="blc"
                     /locus_tag="ACHKAY_001828"
     CDS             32469..33002
                     /gene="blc"
                     /locus_tag="ACHKAY_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5700512.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32999..33316)
                     /gene="sugE"
                     /locus_tag="ACHKAY_001829"
     CDS             complement(32999..33316)
                     /gene="sugE"
                     /locus_tag="ACHKAY_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5700513.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33498..33644)
                     /gene="ecnB"
                     /locus_tag="ACHKAY_001830"
     CDS             complement(33498..33644)
                     /gene="ecnB"
                     /locus_tag="ACHKAY_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5700514.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33755..33880)
                     /gene="ecnA"
                     /locus_tag="ACHKAY_001831"
     CDS             complement(33755..33880)
                     /gene="ecnA"
                     /locus_tag="ACHKAY_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5700515.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33932..34498)
                     /gene="efp"
                     /locus_tag="ACHKAY_001832"
     CDS             complement(33932..34498)
                     /gene="efp"
                     /locus_tag="ACHKAY_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5700516.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34540..35568
                     /gene="epmB"
                     /locus_tag="ACHKAY_001833"
     CDS             34540..35568
                     /gene="epmB"
                     /locus_tag="ACHKAY_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5700517.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35958..36827
                     /gene="yjeJ"
                     /locus_tag="ACHKAY_001834"
     CDS             35958..36827
                     /gene="yjeJ"
                     /locus_tag="ACHKAY_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO5700518.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         8860 a        10101 c         9355 g         8648 t
ORIGIN      
        1 ttaccagcta tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagacg
       61 aactagctgc gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc
      121 agggaagaaa cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc
      181 aggcgacgca taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg
      241 ctctgataga gtgtatgatc cgcctcgcct gccgtaccga cgatccagat attctcttcc
      301 tgcaacatac gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc
      361 gcgccgcagg ctactttttt cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca
      421 atcaccgcgt gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga
      481 tcggttacac cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc
      541 agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg
      601 gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga
      661 gactcaaggg cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag
      721 acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa
      781 atcatttcgc tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc
      841 ggcctacatg atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt
      901 tcacgccgca tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta
      961 aagagggtat tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg
     1021 cggctatttt ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg
     1081 ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt
     1141 taccgacctg acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc
     1201 gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt
     1261 tgcgctcgtc catattaacc gcttcgactc gaacttccac gcgatcgccc aggcgataag
     1321 tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat
     1381 tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga
     1441 agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca
     1501 tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg
     1561 ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg
     1621 tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt
     1681 gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg
     1741 ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca
     1801 gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt
     1861 agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg
     1921 aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt
     1981 ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa
     2041 ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt
     2101 tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt
     2161 tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact
     2221 gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg
     2281 cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa
     2341 ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt
     2401 tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac
     2461 ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa
     2521 tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa
     2581 agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaga tcaacacggc
     2641 ccgcttttgc ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct
     2701 gcggccagat gtacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc
     2761 ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac
     2821 gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg
     2881 ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt
     2941 cagtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg
     3001 cttcacgacg acctttacgg tcagcgccca gcggctgcgc cagcacctga tcgccatgaa
     3061 tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca
     3121 gaaagccgta gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg
     3181 gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca
     3241 ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct
     3301 catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag
     3361 ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg
     3421 ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag
     3481 cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact
     3541 ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc
     3601 actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat
     3661 cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta
     3721 gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac
     3781 gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag
     3841 cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc
     3901 gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt
     3961 cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa
     4021 ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca
     4081 gaattacgcg tcgaacggat cgcgcagaat catggtttca gtacgatccg gaccggtaga
     4141 gatgatatcg atcggcacac cagtcagctc ttcaatacgc ttgatgtagt tcagcgccgc
     4201 ctgcggcaga ccgctacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt
     4261 ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg tcgtcacttc
     4321 acgaccatcc ggcatacggt aagccacaca gagtttcacc tctttcaggc catccagaac
     4381 gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc
     4441 aacggtgtcc agccagccgg tacgacgacg acgccccgta gttgcgccga attcgttacc
     4501 ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gtcgggaacg gacctgcacc
     4561 tacacgagtt gagtaagctt tgaggatacc cagaacataa tcaacataac gcgggcccag
     4621 gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt
     4681 accgtggtcg atatccagca gcgtaccctg cgcaccttca aacatgacga aatcgccacg
     4741 ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc
     4801 aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt
     4861 aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt
     4921 gtcgaaaagg tcgccaacac gcagaccgcg acgtgctact ttatcttcat aagcaggccc
     4981 gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc
     5041 cagcgcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg
     5101 aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag
     5161 cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag
     5221 atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg
     5281 gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc
     5341 gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt
     5401 gcttaaaaat ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa
     5461 tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca
     5521 caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg
     5581 tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca
     5641 gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa
     5701 aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt
     5761 tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc
     5821 tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga
     5881 tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg
     5941 catcgccttc accacgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca
     6001 cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac
     6061 gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg
     6121 tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac
     6181 tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt
     6241 cggcaatggc gttatctgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg
     6301 agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcagtg acgatatctt
     6361 tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca
     6421 cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga
     6481 tgcgccattt gatgtaagaa tcgacgatca ggtctttctt ctctttggtc acaaagcggt
     6541 cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg
     6601 gtatcttgaa atgcagaccc ggctcataaa ccagaggttt gttgtcatcg tcacgcagta
     6661 ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt
     6721 aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc
     6781 ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg
     6841 actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg
     6901 cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat
     6961 ctggtctaac ggcagaacca tcaggtttcc acctttgtcg ttaaccagca ctttgcgggt
     7021 gttgcccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc
     7081 ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat
     7141 ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg
     7201 cggctgaact tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg
     7261 cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa
     7321 gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg
     7381 ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt
     7441 accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt
     7501 cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg
     7561 cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc
     7621 cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac
     7681 cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata
     7741 gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc
     7801 gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc
     7861 gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg
     7921 cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct
     7981 tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct
     8041 ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc
     8101 ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc
     8161 gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc
     8221 actctttttc tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggtg
     8281 gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg ccccgtcaaa gcctgaaaaa
     8341 gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt
     8401 cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca
     8461 gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt
     8521 cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct
     8581 cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta
     8641 cggtatctgc aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt
     8701 cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa
     8761 gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga
     8821 tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgt gactgtatct
     8881 gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac
     8941 gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag
     9001 ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt
     9061 gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct
     9121 ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg
     9181 tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt
     9241 gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca
     9301 gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca
     9361 gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc
     9421 agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa acagcccgaa accttattcg
     9481 gtttcttcgc tgtcctgttg cgcggaagta ttctgcgcgc tgctaccatg atggtagtta
     9541 ctgctggtac cgccaccggc gttgttactg tgatgagaaa ccgggcgaga cgggacaaca
     9601 gtagaaatcg cgtgcttgta aaccatctgg ctgaccgtgt ttttcaacag gatcacgaat
     9661 tgatcaaaag actcgatttg cccttgcagc ttaataccat tcaccaaata aatagaaact
     9721 ggaacacgtt cccgacgcag tgcgttcagg aacggatctt gtaaagattg ccccttagcc
     9781 attctctctt ttccttatat gcttatttgt actttgaacc tttcgattct gaaaaaattg
     9841 cgcacgatac gtctcaattg tacacattca gcctgcgata gcaccaacaa cctgtaatac
     9901 ttcgtcgcgc gcctgttctg gtttttcact gtcaagccag tgaacccctt cccaaccacg
     9961 cagccaggtt atctgccgct tcgccaactg tctcgtggcg caaacacctc gataaaccat
    10021 ttcatcgtat gagatttcgc cttcaaggta agaccacatc tggcgataac ccacgcaacg
    10081 aatggaaggc aagtccgtat gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc
    10141 aaaacctgaa gccaacatct gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg
    10201 gctcgccggg gcgatggcga actgatgcac ctgatacggt agagcgtctc ctgacgtttg
    10261 cgtcagttcc gttaaagttt tacccgaaat gaaaaaaact tccagtgccc gggaaagcct
    10321 ttgtggatca tttggatgaa tccttgctgc cgcaaccgga tctacctcct gaagttgacg
    10381 atgcaatgac tcccaacctt gctctaccgc ctgttgctca attctggccc gcacttccgg
    10441 gtctgccgac ggtagcggcg acaacccttc cagcaatgcc ttgaaataca acatcgtacc
    10501 gcccactaac agtgggatcc gccccgccgc ggtgatatcg gccatttccg ccagcgcatc
    10561 gcggcgaaaa tcagcagccg agtaagcctg cgacggatcg cgaatatcca gcaatcggtg
    10621 cggcgcggcg agtaactctt cagcgttcgg cttcgccgtc ccgatatcca tccctttgta
    10681 aataagggca gaatcaacgc ttatcaactc tactggtaaa attttacgca gctcaatggc
    10741 taacgccgtt ttaccggagg ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc
    10801 cttactgata tcactcatct ttcagggctt ttatcgccgg atgtaaatca acagattgta
    10861 acagaccacc cggcggcgtt ttcacaagtt gcggacataa ccgttccacg tccgccagca
    10921 gggttatggc ctgtgccatt gaccactgcg catgttcgct catcagattt cgtgcaatcc
    10981 actgcgcaat attgccaggt tcgaatacgg actgcttcgc caggtagcct atcagttcag
    11041 gaatcaagat ttgtaaattt tgttggcgta agggtaaagg cacggccctg atggtcacat
    11101 gctgtgcatc tgactggaaa tcaataccca attccgccag ggcagactgc gctttttcta
    11161 atgccgattt ttcttcgcca gaaactttta gccgcaacgg aatcagcaac ggctgggcgc
    11221 aaactggcgc ttcacccggc gtcagttgcg cctgacgcag ccaacgttct gccactggca
    11281 aggctaaaag tgaaatgttg ccatcgcgct ccagcaacgc acagtcggaa tggacgatag
    11341 tcagtacccg accaaaactc tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg
    11401 cttttggttt ttgcatcggc gcgggcgttt gcaaaagctg gcgatacact tcaccttgct
    11461 gtttctggta gcctggctgc gcattcggcc agggggcagc cggacgactg cctgaggcgg
    11521 gcgcaggaga gtagcgcgga gctaccggct cacgagctgc cggttctgca aagtgattgc
    11581 gccccgccgc cacgcggttt tccggaatgg gacgcggtgc aggttggggt tcatcgtcca
    11641 gcggtagcgg cgtttccagt tgctgttgta gcacgctcag cacgccctga tagataaaat
    11701 catgcaccag acgcgactga tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt
    11761 ccacctgatg cgggtcgatc tccagataca acacaaacgc cggttgttga tcggccccca
    11821 gtttgtcttc gcaggcctgg cggatcgcat gattgatcag gcgatcgcgc atcatgcgac
    11881 cattcacgta gcaatactga atttccgcca gtgcgggcgt agtgtgattt ggatcggcca
    11941 cccagccgcg cagcgtgaga tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa
    12001 aagcggtgcc acaaatcgcg cctaagcgcc gttctttttg cccgccttcc ggcactgcgc
    12061 ggtactgacg cacaatttta ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca
    12121 gcgcaatgcg gcggatgatt tcatcaatat ggctaaattc ggttttctcg gtgcgcagga
    12181 atttgcgccg cgccggggtg ttgtagaaca gatccagcac ctccagcgtc gtccccacag
    12241 gatgcgccgc cggttttacc gtcacgttca tatcgcgccc ttcggcatag gcctgccagg
    12301 cttcctgctg ttctgcggtg cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg
    12361 ccagcgcctc accgcgaaag cccaggctga taatggcttc gagatcgtcc agagaggcga
    12421 ttttactggt ggcatgacga gccagcgcca gcgccagctc atcttttttg ataccgcagc
    12481 cgttatcacg aatgcggata agtttcgccc caccgcgttc gatatcaata tcgatacgcg
    12541 tcgcacctgc atcgaggctg ttttccacca gttctttgac taccgacgca ggtcgctcga
    12601 ccacctcacc tgcggcaatc tggttcgcca gttgtggcgg taagacctga attggcatca
    12661 atcgtcctta gtttggcagc gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg
    12721 ttgccccctg cggcgcagat tgcatcggat gcgcaaggaa ataattgcgc aggcctttgt
    12781 aaatggcttc tgccagctgt tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt
    12841 tgttgctgat aaaaccggtt tcgaccagta ctgacgggat atccggcgaa cgcagaacgc
    12901 cgagactcgc gtgttctggt cggcgtttat gtatttcgcc aatgcgttgc aactgactga
    12961 tcatactggt cgctacatca taccctaccc gttgggaatg accgaactgt aaatccagca
    13021 ccgcctggct taaatagggg tcagactgac tgttcgccag cacatcaccc gccccaccca
    13081 gcagctccga ctgtttctcg tgctgctcca gccagctggc catttcgctg ttggcgcgac
    13141 ggttagagag cacccatacg gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt
    13201 gaatcgacac gaggaaattg gcgttttgct tacgtgccac atcactgcgc cccatcaccg
    13261 agataaagta atccccgtca cgggttaaaa cgcccttaaa catcggatcg tcattgagca
    13321 aagtacgcaa tttacgcgcg atggcgatgg tgacattttt ctcccgcgta ccaccggggc
    13381 cgatagcgcc agggtcctga ccgccgtgtc cggcatcaat agcgatgata attttatcgc
    13441 cagtgttagc cgtcgcgcgc gctgccggac gcgttaccgt attactgctg ataacacccg
    13501 tagtgcggtt actttccgtt ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga
    13561 caaccgcagg cgtttcaacg cgtttcgcaa ccacgggcgg cggaggagga ggtggcggcg
    13621 catcggcgtt aatcgtaaag acgacagtgt aattgctgcc attctgccgc ttcaccgctt
    13681 cggttttacc gttttcggta agatcgacca ccagccgtag cgtttgtgca tctttaggcg
    13741 ttccagagcg aatcgccttc accagattat tgccgctgaa caacaacggc agtccctgaa
    13801 tcacgcccgt ttgtttgata tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat
    13861 aatcaggatc gccaataaaa ctcaacgtta tccgcgcctg ttgattaccg ttagaaacct
    13921 gaatatcaga gagcgtcgcg gcacccaccg gcgtgcacag cagcagcagc gtcgctacca
    13981 accaatttct gatgcgatac atcatcccgc cacctttcaa aggttaaccg gctaaacgcg
    14041 ccagcaacaa ttcacccgca gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct
    14101 gataatcaat gtgtatttcg acatccgggt caggaagaac acctgtacct tgttgtggcc
    14161 actccaccag gcagatggca tcgttggcaa aataatcgcg gatccccata aactccagct
    14221 cctcgggatc ggcaaggcgg tacaaatcaa agtgataaac cattaaattg tcgagcgtat
    14281 agggttcgac cagcgtataa gtggggcttt tgacattacc ctgatgaccc agagcttgta
    14341 aaaagccccg gctaaaggtg gttttacctg cgcctaaatc gccatacaga tagattacgg
    14401 ttgcgccatc gcaggctttc gctacccgct cgcccaggtc taatgttgcc tgctcatcag
    14461 ggagcggaat tactcgattc atcatggttt ttatcagtca cttccgggtt aacaatacgc
    14521 tgtagcgtgg aaaagagatc ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt
    14581 acgtcagctg cagcaccgtg cgcgacacag cccgcacagg cggcatcata cggcgacagt
    14641 ttttgcccaa gcaatgcgcc aataatacca gagagcacat cgcccatgcc gccgctcgcc
    14701 atgcctgcat ttccggcatc aataatgcct aaagcgtcag gatgggcggc gaccacggtt
    14761 ccggcacctt tcagcaccgc tacgccgcca taacgttgta ccagacgttt ggcgcaatgt
    14821 aagcggtcac tttcaatttc agcgacggaa cagcctaaca accgtgcggc ctcgccagga
    14881 tgcggcgtga tcacgcgatt gtgacgctta tcgggattga ttgccagcag gttcaatgca
    14941 tcggcatccc acaacatcgg tttgcgaaaa ttctcaactt tttgcagtgc ttttttcccc
    15001 cactcttgct ggcccagacc gggaccaatc accaccacat cggcccattc caggctttcg
    15061 gtaagagagt ccatcgtcag ttcatgcacc atcaattccg gtcgtgcagt cagcagcggc
    15121 gcaatgtttt cactgcgggt cagtactcgg actaaaccag caccagcacg tagcgccgct
    15181 tcccccgtca tacgaatagc ccccgccgtg ccgtgatcgc caccgataat taccagccgc
    15241 ccgtgatcgc ctttatgcga agtcgggcga cgcggtttta gccagtgaga aagttgttct
    15301 gccgaaaacc gctgaatttt cgtctcctga cctgccagcc aactatccag ccccagtgag
    15361 tcaaaatgca gttgtccggt aacatcccgc gcttttccag tgagcaagcc tggtttcagc
    15421 gcaataaaag tgatggtgtg atcggcgttg atcactgcgc ctggcgtagc gccggtttca
    15481 gccagcaggc cggaagggat atcaaccgcc gcaatcggcg caggatggga attagcgtgg
    15541 tcgattaact ggctaatgga ttcgcggggc gcttgctgca agccggtacc gagcagcgca
    15601 tcaacaatca gatctaccga ttcgggccag acaatattcg aagcatggat ctcgcctccc
    15661 gcgtttaacc atgcttcgcg tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc
    15721 tgggccaaca acgtgacctc aatgccgacc gctgtggcca gtcgcgcgac cacgtagcca
    15781 tcgccgccgt tattaccatg accgcacagc accagccagt ggcgggcgtc aggatacgcc
    15841 gaacgacaca cctggaatgc ggcttcgcca gcgcgaagca tcagctcata gagtgtaagc
    15901 cccagcgcat ctgccgcctc acgttctccg cggcggatat cgtcggcgtg ccagacggtg
    15961 tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga gcccctcgat
    16021 ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg aactgggctt tcagcaggta
    16081 ggtattaccg ataccgatct cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa
    16141 caataccacg gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc tcgcccccat
    16201 gagttattac ccggtacgct gcgcgtgatc agcgtgcgga tgaattacct tcctgctaac
    16261 gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg ttatgcgctg
    16321 ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga gatgattcag
    16381 caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc
    16441 ccgttagctg caaaagctgg gctcggctgg acaggtaagc actcacttat cctcaatcgc
    16501 gaggccggtt ctttcttctt tttaggcgaa ttgctggtcg atattccgct gcccgtggat
    16561 caaccagtcg aggaaggatg cggtaaatgc atagcctgta tgacgatttg cccgaccggt
    16621 gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct caccatcgaa
    16681 ctggaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat ttacggttgc
    16741 gatgactgcc agcttatctg cccgtggaat cgctattcgc aactcactac agaagacgat
    16801 ttcagcccgc gtaagccgct acacgcaccg gaactcattg agttattcgc ctggagcgaa
    16861 gagaagtttt taaaagtcac ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg
    16921 ctgcgtaata tcgccgtggc attaggcaat gccccctggg atgaaacgat tctggcggcg
    16981 ctggagagtc gcaaaggtga gcacccactt cttgatgagc acatagcgtg ggcgatggcg
    17041 cagcaaatcg agaggcgaaa tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt
    17101 ctggttcggg tgattgaaaa agggttacca cgtgacgcct gattcatcca cagcttgtga
    17161 ataaaaataa aaacgcattg caattcaaga cgaataaaat cgtcaagtga tcgactcgac
    17221 aaaacgaaat cttaattttt aacttatcta tcaataagtt aaaaaaatac tattcaccgt
    17281 gcgaagtttc tttgaaatta agactacagc ctgaggctgt ggataactct gtttacaaga
    17341 gtttgtcaga gacaacaaaa accagcaata acgcatcgcg tcgctgtgga tattttattg
    17401 agagaagaat ttggagcggg aaacgagact cgaactcgcg accccgacct tggcaaggtc
    17461 gtgctctacc aactgagcta ttcccgcttg ggtggtctgt gccttacagc actttcaaat
    17521 tttggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac
    17581 caactgagct attcccgctt aatcttcgtc tttcgaaccg ctactgcctt ggctgccttc
    17641 actcacctta gtcacatact tttgtaagtt cccaaggatt cgttcagttg ccgccttgtc
    17701 gcaattcgaa atactgcgat tgggtggtct gtgctttgca gcacttttca aattttggag
    17761 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
    17821 gctattcccg cgtactactt aattttgctt tcgtaatttt cgcatttctg cgtcgttacg
    17881 ggaggcgcat tatacgagaa atctttccct ctgcaacccc ccaaaaaagt tttttttgcg
    17941 tttttatctc aagtgctgaa attatcagca agacgttcaa tttagcgtca aaatcacgcc
    18001 gccgcaagtg gattccggcg cttacgtgac caggaaaaat tttacagctt gataaaatgc
    18061 tcgcggtagt aagccagctc cgccaccgat tcacggatat catccatcgc ctgatgcgtc
    18121 ccctgcttgg taaaaccatc cagaatttcc ggcttccagc ggcgcgccag ctctttcagg
    18181 gtgctgacat cgagataacg gtagtggaag taggcttcca gctccggcat gtatttaaac
    18241 aggaaacgac ggtcctgacc gatgctgtta ccgcaaatcg gcgattttcc cgcaggcacc
    18301 cactgtttta aaaattcgag cgttgccagt tcagcttccc gatcgcccat cgtgctcgct
    18361 ttcacgcgct ctaccagccc gctggcggta tgggtgcgca cgttccagtc atccatcagc
    18421 gccagctgtt catcagactg gtgtactgca atggtcggcc cttctgccag aatattcagg
    18481 ttggcatcgg tcaccagcgt ggcaatctca ataatgcgat cgcgctcggg atccagaccg
    18541 gtcatctcaa gatcgatcca aatcaggttg ttttcattgg cactcatgct attttccacc
    18601 cattcacagt gactatattg atccacaatt gcgtgtatca tagatgtttt gcccatcagg
    18661 ggcgaccagg agtctgtacg attgagtaaa aataaactct ccaaaggcca gcagcgccgc
    18721 gtgaacgcca atcaccagcg tcgtcttaaa acgtctaagg agaagcccga ctacgacgac
    18781 aatctgtttg gcgagcctga tgaaggtatc gtcatcagcc gctttggtat gcacgctgat
    18841 gtggaatccg ccgatggcga cgttcaccgc tgcaatattc gccgtaccat ccgttcgctg
    18901 gtaaccggcg accgcgtagt ctggcgtccg ggtaaaccgg cggcggaagg cgtaaatgtc
    18961 aaagggatcg tggaagcggt gcatgagcgt acctcggtgt tgacgcgtcc ggatttctac
    19021 gacggcgtaa aacctattgc cgccaacatc gaccagattg tcattgtctc cgccattttg
    19081 ccggagctgt cgctcaatat tatcgaccgt tacctggtgg cctgcgaaac cttgcagatt
    19141 gagccgatta ttgtgctcaa caagatagac ctgctggacg acgaaggcat ggcgttcgtc
    19201 aacgagcaga tggatatcta ccgcaatatc ggttatcgcg tattgatggt ttccagccat
    19261 actcaggatg ggctaaaacc gctggaagag gcgttgaccg ggcgcatcag catttttgcc
    19321 gggcagtctg gcgtcggcaa atccagcctg ctgaatgcgt tgctggggct gcaaaaagag
    19381 atcctgacca acgatgtctc tgacaactcg gggctcggtc agcacaccac gaccgccgct
    19441 cggctgtatc acttcccgca cggcggtgat gtgattgatt cccctggtgt gcgtgagttc
    19501 ggcctctggc acctggagcc ggaacaaatc actcagggct ttgtcgaatt ccatgactat
    19561 ttaggtctgt gtaaatatcg cgattgcaaa cacgataccg atccgggctg cgctatccgg
    19621 gaagcggttg aggaagggaa aatcgcggaa acccgtttcg aaaactatca ccgtattctg
    19681 gaaagcatgg cgcaggtaaa aacgcgtaaa aacttttctg atacggatga ctgacaatta
    19741 agctaaccgt cgttagaatc gtcccctttt tttcaggatc cggcatgttg gccggatcag
    19801 gaacgacaaa acaatggcct ggaggctacc ttgttaaatt catttaaact ttcgctacag
    19861 tacattctgc cgaaactatg gcttactcgc ctggcgggtt ggggcgcaag caagcgggca
    19921 ggatggctga caaaactggt tatcgatctg ttcgttaaat actacaaggt cgacatgaaa
    19981 gaggcgcaaa agccggacac cgccagctac cgcaccttta acgaattctt tgtccgtccg
    20041 ctgcgtgacg aagtacgccc aatcgatacc gatccgaatg tcctggtcat gcctgccgat
    20101 ggcgttatca gccagttggg taaaatcgaa gaagataaaa tcctgcaagc caaaggccac
    20161 aactacagcc tcgaagccct gctggcaggc aactatctga tggcggacct gttccgcaac
    20221 ggtacgtttg tgaccactta cctctccccg cgtgactacc atcgcgtaca catgccgtgc
    20281 aacggtattc tgcgtgagat gatctacgtg ccgggcgatc tcttctccgt taaccatctc
    20341 accgctcaga acgtgccgaa tctgtttgcc cgtaacgaac gcgtgatttg cctgttcgat
    20401 accgaatttg gcccgatggc gcagattctg gtcggggcga cgattgtagg cagcattgag
    20461 acggtctggg cgggcaccat tacgccgccg cgcgaaggta tcatcaagcg ctggacctgg
    20521 cctgccgggg aaaacgacgg ttctgtggca ctgctgaaag gccaggaaat gggccgcttt
    20581 aaactcggct ctaccgttat caacctgttt gcaccgggta aagtgaatct ggttgagcaa
    20641 ctggaaagcc tgtctgtcac gaaaattggc cagccgctgg cagtatctac cgaaaccttt
    20701 gttacgccag acgctgaacc cgccccactt cctgctgaag agatcgaagc agaacacgac
    20761 gccagcccat tggttgacga taaaaaagac caggtctaat ccatcaaagg aaacgctgac
    20821 gtgcgcctga ttatcacttt tctgatggcc tggtgcctca gttggggggc gtacgccgcg
    20881 acggcccccg atagcaaaca aatcactcag gaactggagc aggcaaaagc ggcgaaaccc
    20941 gcacagccgg aagtcgtaga ggcgctccag tctgccttaa atgcgcttga ggaacgaaaa
    21001 ggttcccttg agcgcatcaa acaatatcag caagttatcg ataattatcc gaaactctcc
    21061 gctactctgc gcgcacaatt aaacaacatg cgtgacgagc cgcgcagcgt gtcgccggga
    21121 atgtctaccg acgcgctgaa tcaggaaatt ctccaggtca gcagccagtt gctggataaa
    21181 agccgtcagg cccagcaaga gcaggagcgc gcccgcgaga ttgccgattc gctgaatcaa
    21241 ctgccgcaac agcaaaccga cgcccgccgt cagttaaatg agatcgagcg ccgcctggga
    21301 acgcttactg gcaatacccc gctcaatcag gcacaaaatt tcgcgttgca gtctgactcc
    21361 gcacgcctga aagcgctcgt tgatgaactg gaactggcgc agctgtctgc caataaccgt
    21421 caggaattag cgcgcttacg ctcagagctg gcggaaaaag agagccagca actggatgcg
    21481 tatttgcagg ccttacgtaa tcagttgaac agccaacgtc agcttgaggc ggagcgggcg
    21541 ctggaaagta ccgaattgct ggcagaaaac agcgccgatt tgccgaaaga catcgtcgcg
    21601 caattcaaaa ttaaccgcga actatcggcg gcactgaatc aacaggcgca gcggatggat
    21661 ctcgttgcct cgcaacagcg ccaggccacc agccagacgt tacaggttcg gcaggcgctg
    21721 aatacgctgc gtgaacagtc gcaatggctg ggatcgtcca acctgctcgg cgaagcgttg
    21781 cgggcgcagg tggcacggct gccggaaatg ccgaaaccac aacagcttga taccgaaatg
    21841 gcgcagttgc gtgtgcaacg gttacgttat gaggatctgc ttaataaaca gccgctgcta
    21901 cggcaaattc atcaggccga cggtcagccg ctgaccgccg agcaaaaccg tattctggaa
    21961 gcacagctac gcactcagcg tgagttgctg aactcattgt tgcagggtgg cgacacgcta
    22021 ctgctggaac tgaccaagct gaaagtctcc aacgggcaac ttgaggatgc gctgaaagag
    22081 gtgaatgaag cgacgcatcg ctatctgttc tggacctctg acgtgcgccc gatgaccatc
    22141 gcctggccac tggaaatcgc ccaggatctg cgtcgtctca tttcgctgga caccttcagt
    22201 cagttgggca aagccagtgt gatgatgctg accagcaaag agacgatttt gccgctgttt
    22261 ggcgcgttga ttctggtcgg ttgcagtatt tactcgcgcc gctatttcac ccgttttctt
    22321 gaacgttcgg cggcgaaagt cggcaaagtg actcaggatc acttctggct gacgttgcgc
    22381 actcttttct ggtcgattct cgtcgcgtca ccgctgccgg tgctgtggat gacgctgggt
    22441 tacggcttgc gcgaggcgtg gccttatccg ctggcggtcg cgattggcga tggcgtaacg
    22501 gccaccgtgc cgctgctgtg ggtagtgatg atttgcgcca cctttgcccg cccgaacggc
    22561 ttgtttatcg ctcattttgg ctggccgcgc gaacgtgttt cccgtgggat gcgctactac
    22621 ctgatgagca tcgggcttat tgtgccgctg attatggcgc tgatgatgtt cgataacctc
    22681 gacgaccgtg aattctccgg ttcgctggga cggctttgct ttatcctcat ttgcggtgcg
    22741 ctggcggtgg tcaccctcag cctgaaaaag gccgggatcc cgctgtacct caacaaagag
    22801 ggcagcggcg acaacattac caaccatatg ctgtggaaca tgatgattgg cgcaccactg
    22861 gttgccattc tggcgtcagc ggtgggttat ctggcaacgg cgcaggcact gttagcgagg
    22921 cttgaaacct cggttgccat ctggttcctg ctactggtgg tttatcacgt tatccgccgc
    22981 tggatgctga tccagcgtcg caggctggcg tttgaccggg cgaagcatcg ccgggcagag
    23041 atgttagcgc aacgcgcgcg tggcgaagag gaagcgcatc atcacagtag cccggaaggg
    23101 gcaattgaag tcgatgaaag cgaagtcgat ctcgatgcca tcagtgcgca atccttgcgg
    23161 ctggtgcgct caattttgat gttgatcgcc ctgctttctg tcattgtgct gtggtcagaa
    23221 atccattccg ctttcggctt cctcgaaaat atttcgctgt gggatgtcac ctccacggta
    23281 cagggcgtag aaagtctgga gccaattacc ctcggtgcgg tgctgattgc cattctggtg
    23341 tttatcatca ccacgcagct ggtgcgcaac ctgcccgcgc tgctggaact ggcgattttg
    23401 cagcacctgg atttaacgcc gggtacgggc tacgccatca ccaccatcac caaatatctg
    23461 ctgatgctga ttggcgggct ggtcggcttc tcgatgattg gtattgagtg gtcgaaattg
    23521 cagtggctgg ttgccgcgct cggtgttggt ctcggttttg gtttgcagga gattttcgcc
    23581 aactttatct ctggcctgat tatcctgttc gaaaaaccga ttcgcattgg cgatacggta
    23641 acgatccgcg atcttaccgg tagcgtgacg aaaattaaca cccgcgccac caccatcagc
    23701 gactgggacc gtaaagagat aatcgtgccg aacaaggcgt ttattaccga gcagtttatc
    23761 aactggtcgc tctctgactc ggtcacgcgc gtggtgttga cgataccggc ccctgccgat
    23821 gccaatagcg aagaagtgac ggaaatcctg ctcaccgcag cgcgtcgctg ctcgctggtg
    23881 atcgacaacc cggcaccgga agtcttcctg gtggatctgc aacaggggat tcagattttc
    23941 gagctgcgta tttacgccgc tgagatgggt caccgtatgc cgctacgcca tgagatccac
    24001 cagctgattc tggcaggctt ccacgcccac ggtatcgata tgccattccc gcccttccag
    24061 atgcgtctgg aaagcctcaa cggtaaacaa acggggagaa cgctgacgtc tgcgggcaaa
    24121 ggtcgtcagg cgggaagttt gtaagtttgt gtgccggatg aaccaatccg gctcacaaac
    24181 aaaactgata gcgcagaaaa aactgccaca gccagtagcc gaaaatagcc agcgcggtgg
    24241 cgtacataaa ccagccgcgt ttttttcgcc acaaagcgaa ccagagaaaa gggaaaaaga
    24301 gcggtaacgt cattatcaca ccaacatcac gggtatcgtc ttcttcgaga tacatcaaca
    24361 catcgcagac gttgttaatc ccgcgtccat caatcatcca ctctttgtcc agcgcggaag
    24421 tgatggttat ccagaggaac gccacaataa accagatgca gcataagaca aacatgcgcg
    24481 cactcataag gtaaccggtc gcttaatttc gctatagcgc atacgaaaca aatcgccgta
    24541 tcccactaat tccccgcgaa aaaccagaca tcggataaga taagcgcaat aaatatccgg
    24601 caggtttccc caccgtttac catcgcgatc tttgagggtg ttaatggtgc tacccaccac
    24661 catcgccact ttacgggtgt ggcaggcatc gacaaaagag agaatcgtct gctctatttc
    24721 cgctacatcc ccggcagaaa attgcagcgc cctgatttca tcttcggtta ttgccgggtc
    24781 ccggctggtg tcatccataa catttcctgt ctttacgccc tatcgtcagg tagacgatag
    24841 ggcatcgggt tattccaccg cccactgcgg attttttgcc actctgctgc aatagttctg
    24901 gtaaatcgcc atcgctaaca gcgagaagaa gacagggccg ccaatcatcc acaatgtgct
    24961 gtcccagtct ccggcttcaa ccacaggttg aatgatggtg aagacgttcg caaatgtcac
    25021 caccagtacg acaaccactg ttgcaatcat tgccgacata tgcgttttga aaatcacaaa
    25081 tggtctgtcg agatcctgac gtgctttaaa aaatgggaaa gccagcgcga ggaacaggta
    25141 aggaagcgtc atagacacgt tcgccatcag cgtcagcttg ttaaagaacg ccgatgcggt
    25201 accgccacca aacgaaacca gcaggatgaa gacagtaacc aacccgcact gcatccacat
    25261 ggcgatagac ggcatcccca tcgcattcag gcgcgtcatc ggttccggcc acaacgcttt
    25321 cggcgtcccc tggatgatgg ctttcagcgg tgaatagcaa agcgtaaaga acgcaccggt
    25381 ataagcgagg aacatcgaaa gtccggtaat acgcgcaaac catacgccca gcgacaatga
    25441 cgcttcaggt gacaaatgca gtgcgttacc cagcgtcacc ccgaggctct tcatcagcac
    25501 ataggtaata ttgccaaggt taacggaacc attacttaat acctgctgcc agtttgtgct
    25561 gacgccccat aaaaatattg ccagcgaata accgattgaa ataacaatag cggcaaaaac
    25621 aatacctttg gcaaagttct tttctggatt ttccgtttta tcgaccagac caccaaccgc
    25681 ttcaattccg ccataggcaa aaatagcaaa taccacaaac gatagcattg ccagaccgga
    25741 ctgataacct gggttcggtg atgcaaggaa attaatatcc tgcgcgaaat gcccaccatt
    25801 taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca ttactgcaat
    25861 accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa cgacggtgac
    25921 cagaatcatc catgccactg ccagcagacc aaccacctgc gtaggctcca gtccagcaat
    25981 acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta cccaaacttt
    26041 cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg taccaatgaa
    26101 ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccgcctt cttctttgcg
    26161 ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata acaatgcaga
    26221 aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg ggctattggc
    26281 aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca tcttttttat
    26341 cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc ccactgaact
    26401 gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata cctggatgat
    26461 atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat atgctacaga
    26521 atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa ttatgcccgg
    26581 tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa catcaccaga
    26641 cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa ggcttcaatc
    26701 agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg gttatcttgt
    26761 tcaaagcgtt gttgctgctc acgggcatcc gtcaattcat ggaaaccatt cgccagctca
    26821 atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt actgatttgc
    26881 gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc tttgccaata
    26941 tttggctcta cgccaaaggt aaacagcaat tgtagcagcg tgtcgcggtc ttcttcggta
    27001 tcagcaacat tgctcaaatc cagtttcgct gcgacttccc gcagttgcgt tttgtcggca
    27061 gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag gctttctgct
    27121 gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa ccggtacata
    27181 tcatagtgcg gtcgatacca ctccagcata gtgaactcag ggttgtgata acgccccatc
    27241 tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc aaccagcagg
    27301 cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg cgaatgcccg
    27361 gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt cgcctggctc
    27421 atacaaggcg tctccacctc cagcactcca cgatcggcaa agaaacgacg gatctccgcc
    27481 ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca ggatgccgtt
    27541 tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg caacgcgacg
    27601 gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa aaagcacgat
    27661 ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc ttgcgcacta
    27721 aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt gtacctataa
    27781 aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct gaagtacggg
    27841 gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc gatcttgcca
    27901 ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag gcaaatccaa
    27961 atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc gttgctgcag
    28021 aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac tttcacgata
    28081 cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc gtccaccact
    28141 gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt cgcccggatg
    28201 gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg ttcgccgccg
    28261 ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa ttcccgcaga
    28321 tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt catgttcgcg
    28381 gcgtggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct aacgcggtcg
    28441 ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc ggcatcgtta
    28501 ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac atggaattcg
    28561 ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa ggctgccgcg
    28621 gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat tacggcatgg
    28681 gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt ccacgcgaca
    28741 aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc acgccgcgtg
    28801 gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa aaaactgcat gaacgtctgc
    28861 cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa gaaccgattc
    28921 cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag aactgtgaaa
    28981 cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg cacggtgcaa
    29041 accgtctggg ttctaactcc ctggcggaac tggtggtctt cggccgtctg gccggtgaac
    29101 aagcgacaga gcgtgcagca actgccggta atggcaacga agcggcaatt gaagcgcagg
    29161 cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc gaaaactggg
    29221 cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg ttgcggtatc taccgtacgc
    29281 cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc ttcaagcgcg
    29341 tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc attgaactgg
    29401 gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt aaagagtccc
    29461 gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc aacttcctca
    29521 aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac agcgacgtga
    29581 agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat gcagccgata
    29641 aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa cctgaaaatt
    29701 gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt ctatgaagtg
    29761 ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga caacctggca
    29821 ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg cggcatgatg
    29881 gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac cgacggtatg
    29941 aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga tatgacccac
    30001 ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg caccgcggat
    30061 cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt ctccggttgc
    30121 atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc agagttcatc
    30181 ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga ccacggtaag
    30241 aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac tttcgtgggc
    30301 tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca gcagggcaaa
    30361 gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg agtgcaacat
    30421 gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga aaaaattgcc
    30481 gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt ggttcagcat
    30541 tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gaagcctggg cgggattcgt
    30601 cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg cggcagctct
    30661 gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca ttgtaaaaga
    30721 cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg tggttgccac
    30781 catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt aatccaaatc
    30841 caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt atgtggagcg
    30901 caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg gggctgtttc
    30961 cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc attggtcgcg
    31021 tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt atgcaccacg
    31081 cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac ggtctggctg
    31141 ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc gccaatgtaa
    31201 atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt tcaaaacgac
    31261 gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc aacaaatcaa
    31321 cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc ccggtatggc
    31381 ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg cggacatcgc
    31441 caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca gcaaaacatt
    31501 tacgggcgtg cttggtggcg acgctattgc tcgaggggaa atcaagttaa gcgatcccac
    31561 aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac tattacatct
    31621 cgcaacctac actgctggcg gcctgccatt gcaggtgccg gatgaggtga aatcctcaag
    31681 cgacttgctg cgcttctatc aaaactggca gcctgcatgg gcgccaggaa cacaacgtct
    31741 gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt ctggtttgag
    31801 ttttgagcag gcgatgcaaa cacgtgtctt ccagccactc aaactcaacc atacgtggat
    31861 taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag gcaaggcagt
    31921 tcatgtttcg ccaggggcgt tagatgctga aacttatggt gtgaagtcga ccattgaaga
    31981 tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat atcaacgaca aaacacttca
    32041 gcaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt atcagggcct
    32101 gggctgggaa atgctggact ggccggtaaa tcctgacagc atcattaacg gcagtggcaa
    32161 taaaattgca ctggcagcac accccgtaaa agcgattacg cccccaactc ctgcagtacg
    32221 cgcatcatgg gtacataaaa caggggcaac cggcggattt ggtagctatg tcgcgtttat
    32281 tccagaaaaa gagctgggta tagtgatgct ggctaacaaa aactatccca atccagcgag
    32341 agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa aatttcatcg ggtccgaatt
    32401 ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa accagtaagg
    32461 aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt ctggtcgttg
    32521 cctgcagttc tcctacgccg ccgcgtggcg taacagtagt gaataatttc gacgccaaac
    32581 gttatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa cgtggactgg
    32641 aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg cctgaatgtc attaataaag
    32701 gctataaccc tgacagagga atgtggcagc agagtgaagg gaaagcgtac tttaccggcg
    32761 caccaactcg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc ggttataacg
    32821 ttattgcact cgatcgggaa taccgccatg cgctggtttg cggcccggac cgcgactacc
    32881 tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag atgctggcag
    32941 tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag cccggtagtt
    33001 agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga ctcgccaggc
    33061 gcatcgggtt agcggactca ccgagcagca caatgccggt gatggccgcg ccgactgcgc
    33121 caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc gcccaggcaa
    33181 gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc aaacgactaa
    33241 agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta
    33301 agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg aaaagaggcg
    33361 tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa
    33421 tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca tacctgtcgt
    33481 accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
    33541 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
    33601 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
    33661 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgac
    33721 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt
    33781 tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc gtgagcagcg
    33841 tgctggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat
    33901 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
    33961 agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc ttaaccacag
    34021 cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
    34081 gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
    34141 cattccacag agttacgata cactctgcct gatccagcag ccatttagcg ttgtcgccaa
    34201 ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
    34261 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct tcagcggaat
    34321 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
    34381 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
    34441 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
    34501 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
    34561 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
    34621 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
    34681 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
    34741 ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt cgtcgcgtcc
    34801 ggattctcca ccgacccgct ggaagaacag cacagcgtag tgcctggttt gttgcataaa
    34861 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
    34921 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagactgct
    34981 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
    35041 ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc catcccgcat
    35101 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg tatcaccgag
    35161 gcgctggttg aacgctttgc ccgttctacg ctgcaaatct tgctggtgaa tcacatcaat
    35221 catgccaatg aggtagatga aacgttccgt caggcgatgg ctaaattgcg ccgtgtcggt
    35281 gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga acgataacgc acaaacgctg
    35341 gcaaacctga gtaatgcgtt gttcgatgcc ggcgtaatgc cgtattacct gcatgtgctc
    35401 gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg gcagattatg
    35461 cgtgagttgc tgacactggt gtcgggttat ctggtgccga aactggcgcg agaaatcggc
    35521 ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagtaact gattaaacag
    35581 ggaagttaaa cattagcctg atgcgctacg cttatcaggc ctacattgtg cattgaaatt
    35641 gaatggattt gcaatgaact gtaggccgga taaggcgttt acgccgcatc cggcaagagc
    35701 aacaaacgca agtacagcaa cctgtcatta aactgggaaa tttaatcttt gcccgttgca
    35761 taacctttaa ttattcatat acattgctgt tgttactctt tatatagcca acaccaataa
    35821 ttaacctttc ttttctgttt atatagagtg agtacggcta tttatgttat tttaacaact
    35881 attactacca agcccattta tctggcttat aaattaaaat taattattta attgagaaat
    35941 ggttagggag aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca
    36001 agagtaataa tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt
    36061 tgttctttat gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtatgc
    36121 atcaaaagca taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag
    36181 tcattaagaa aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg
    36241 atattaatcg tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct
    36301 tcgtattaac gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg
    36361 aaatgctggc gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat
    36421 tacgtattac ctcactgtta gatttcctgc cgttatatga cgttgactgc caggaaaatg
    36481 gcaatctgga atatgacacc tattcccaac cggaatggaa acataacctg ttcgaccatt
    36541 atctggcggt gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag
    36601 tggtgaaaac gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc
    36661 ttgatttcag ccaacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca
    36721 ccgtagcggc gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc
    36781 acttacgtgt tcaatccacc agcaaaactg caccacaggc gaaataatct catctttgct
    36841 aatgcgaggc tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg
    36901 gatgcggcgt aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg
    36961 cctg
//
LOCUS       DAZFKM010000034        36038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFKM010000034 DAZFKM010000000
VERSION     DAZFKM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHKAY_001835"
     CDS             <1..159
                     /locus_tag="ACHKAY_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO5700519.1"
                     /translation="SLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELMSDPNSS
                     MYEEEYDG"
     gene            152..757
                     /locus_tag="ACHKAY_001836"
     CDS             152..757
                     /locus_tag="ACHKAY_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5700520.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            754..960
                     /locus_tag="ACHKAY_001837"
     CDS             754..960
                     /locus_tag="ACHKAY_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5700521.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            938..1603
                     /locus_tag="ACHKAY_001838"
     CDS             938..1603
                     /locus_tag="ACHKAY_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO5700522.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQG
                     EGA"
     gene            1600..2223
                     /locus_tag="ACHKAY_001839"
     CDS             1600..2223
                     /locus_tag="ACHKAY_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5700523.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            3296..3619
                     /locus_tag="ACHKAY_001840"
     CDS             3296..3619
                     /locus_tag="ACHKAY_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO5700524.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            3603..4079
                     /locus_tag="ACHKAY_001841"
     CDS             3603..4079
                     /locus_tag="ACHKAY_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO5700525.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            4076..4513
                     /locus_tag="ACHKAY_001842"
     CDS             4076..4513
                     /locus_tag="ACHKAY_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5700526.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            4719..5237
                     /locus_tag="ACHKAY_001843"
     CDS             4719..5237
                     /locus_tag="ACHKAY_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5700527.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            5521..5892
                     /locus_tag="ACHKAY_001844"
     CDS             5521..5892
                     /locus_tag="ACHKAY_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO5700528.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            5996..6238
                     /locus_tag="ACHKAY_001845"
     CDS             5996..6238
                     /locus_tag="ACHKAY_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO5700529.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            6318..6743
                     /locus_tag="ACHKAY_001846"
     CDS             6318..6743
                     /locus_tag="ACHKAY_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO5700530.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            6740..8152
                     /locus_tag="ACHKAY_001847"
     CDS             6740..8152
                     /locus_tag="ACHKAY_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO5700531.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            8155..10281
                     /locus_tag="ACHKAY_001848"
     CDS             8155..10281
                     /locus_tag="ACHKAY_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5700532.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDPMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYRKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVVVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            10295..11179
                     /locus_tag="ACHKAY_001849"
     CDS             10295..11179
                     /locus_tag="ACHKAY_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO5700533.1"
                     /translation="MENELIIDGQVIDLSETQENAEETIIQTESQPENESQDDNGKEM
                     ATDPEKTEETPEDYALRIGDEEIQLNADDNDHIDGQPAPQWVKDLRKGFKETQKENRE
                     LRRQLEEALAKHAEHQQPQPDAIPPKPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQ
                     QKLRQQQEYQQRFQQRVEAHKQRAAKLPVKDYQEMEAIVLSELPPIQQEIIIHCADEG
                     SELLAYGLGKSQQLRQRVAAETDPIRAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGG
                     ADAKQDEFNKLCPGAKIE"
     gene            11191..12462
                     /locus_tag="ACHKAY_001850"
     CDS             11191..12462
                     /locus_tag="ACHKAY_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO5700534.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            12505..12690
                     /locus_tag="ACHKAY_001851"
     CDS             12505..12690
                     /locus_tag="ACHKAY_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700535.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            12665..13147
                     /locus_tag="ACHKAY_001852"
     CDS             12665..13147
                     /locus_tag="ACHKAY_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO5700536.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            13156..14574
                     /locus_tag="ACHKAY_001853"
     CDS             13156..14574
                     /locus_tag="ACHKAY_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO5700537.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            14574..15422
                     /locus_tag="ACHKAY_001854"
     CDS             14574..15422
                     /locus_tag="ACHKAY_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO5700538.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            15422..15877
                     /locus_tag="ACHKAY_001855"
     CDS             15422..15877
                     /locus_tag="ACHKAY_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO5700539.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            15880..16572
                     /locus_tag="ACHKAY_001856"
     CDS             15880..16572
                     /locus_tag="ACHKAY_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO5700540.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            16582..17988
                     /locus_tag="ACHKAY_001857"
                     /pseudo
     CDS             16582..17988
                     /locus_tag="ACHKAY_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
     gene            17988..19832
                     /locus_tag="ACHKAY_001858"
     CDS             17988..19832
                     /locus_tag="ACHKAY_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO5700541.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(19847..20332)
                     /locus_tag="ACHKAY_001859"
     CDS             complement(19847..20332)
                     /locus_tag="ACHKAY_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700542.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(20403..20669)
                     /locus_tag="ACHKAY_001860"
     CDS             complement(20403..20669)
                     /locus_tag="ACHKAY_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5700543.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            20791..22914
                     /locus_tag="ACHKAY_001861"
     CDS             20791..22914
                     /locus_tag="ACHKAY_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO5700544.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHLPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIINGAGQTDSTAPLAGNGKWSYVSCGVYIYETDTAINV
                     GIRNYGSGNVLINSPSLCAYGMPSNALQVEKTTFYLSSVPTSGFWDIGERVINSAPAS
                     GQPKAWTCNIPGGPGTYSFLSEGNF"
     gene            22985..24127
                     /locus_tag="ACHKAY_001862"
     CDS             22985..24127
                     /locus_tag="ACHKAY_001862"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5700545.1"
                     /translation="MERKTNKLDSIQVLRGIAVLLVIAFHFRVYLNGVYAQKDLGDIL
                     FGIGEVGVDIFFVISGFIITYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNY
                     EAIYTSSQIIKSYLLIPIDYNFIGPWSGYSIIIPAWTLTYEIWFYVVFFFSLCISHKY
                     RTAIASFLLIIICVLGQLYFHGDVFLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMI
                     IAEAYIRLPSRLINKMPVVQFLNITLAIALLFLCLGLRNGPGIVAWGAYSFMIIAPIV
                     LISKCRDVYRNKILTYVGEISYSLYINHMVVYSLAVLYLKPYGVLDSKGFGVFLAMIC
                     ATFIISIISYNFIEKPSVSIGHAIARRMQKKSDVSSIKTESVREAP"
     gene            complement(24372..25529)
                     /gene="intS"
                     /locus_tag="ACHKAY_001863"
     CDS             complement(24372..25529)
                     /gene="intS"
                     /locus_tag="ACHKAY_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO5700546.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(25691..25765)
                     /locus_tag="ACHKAY_001864"
     tRNA            complement(25691..25765)
                     /locus_tag="ACHKAY_001864"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25730..25732),aa:Arg,seq:cct)
     gene            complement(25841..26773)
                     /gene="yfdC"
                     /locus_tag="ACHKAY_001865"
     CDS             complement(25841..26773)
                     /gene="yfdC"
                     /locus_tag="ACHKAY_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO5700547.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            27067..27822
                     /gene="mlaA"
                     /locus_tag="ACHKAY_001866"
     CDS             27067..27822
                     /gene="mlaA"
                     /locus_tag="ACHKAY_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO5700548.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(28004..29062)
                     /gene="yfdF"
                     /locus_tag="ACHKAY_001867"
     CDS             complement(28004..29062)
                     /gene="yfdF"
                     /locus_tag="ACHKAY_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO5700549.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAK
                     YQNEINQLSAPGVNFDDI"
     gene            complement(29427..30767)
                     /gene="fadL"
                     /locus_tag="ACHKAY_001868"
     CDS             complement(29427..30767)
                     /gene="fadL"
                     /locus_tag="ACHKAY_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO5700550.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            31139..31423
                     /gene="yfcZ"
                     /locus_tag="ACHKAY_001869"
     CDS             31139..31423
                     /gene="yfcZ"
                     /locus_tag="ACHKAY_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO5700551.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            31603..32913
                     /gene="fadI"
                     /locus_tag="ACHKAY_001870"
     CDS             31603..32913
                     /gene="fadI"
                     /locus_tag="ACHKAY_001870"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO5700552.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            32913..35057
                     /gene="fadJ"
                     /locus_tag="ACHKAY_001871"
     CDS             32913..35057
                     /gene="fadJ"
                     /locus_tag="ACHKAY_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO5700553.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            35260..35745
                     /gene="sixA"
                     /locus_tag="ACHKAY_001872"
     CDS             35260..35745
                     /gene="sixA"
                     /locus_tag="ACHKAY_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO5700554.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         9868 a         8175 c         8890 g         9105 t
ORIGIN      
        1 tcccttcaat accagaaaga aagcgtcgag cgagctttaa cgtgcgctaa ctgcggtcag
       61 aagctgcatg tgctggaagt tcacgtgtgt gagcactgct gtgcagaact gatgagcgat
      121 ccgaatagct caatgtacga ggaagaatac gatggctaaa ccagcgagaa ggaaatgcaa
      181 aatatgcaag gaatggtttc acccggcatt ctcaaatcag tggtggtgct gcccggaaca
      241 cggaactcaa ttagcactcg aacgacgaag taaagaacgc gaaaaagcgg aaaaagcagc
      301 agagaagaaa cgacgacgag aggagcagaa acagaaagat aaacttaaga ttcgaaaact
      361 cgccttaaag ccccgcagtt actggattaa acaagcccaa caagctgtaa acgccttcat
      421 cagagaaaga gaccgcgact taccatgtat ctcgtgcgga acgctcacgt ctgctcagtg
      481 ggatgccgga cattaccgga caactgctgc ggcacctcaa ctccgatttg atgaacgcaa
      541 tattcacaag caatgcgtgg tgtgcaacca gcacaaaagc ggaaatctcg ttccgtatcg
      601 cgtcgaactg attaaccgca tagggcagga agcagtagac gaaatcgaat caaaccatag
      661 ccgccatcgc tggactatcg aagagtgcaa ggcgatcaag gcagagtacc aacagaaact
      721 caaagacctg cgaaatagca gaagtgaggc cgcatgacgt tctcagtaaa aaccattcca
      781 gacatgctcg ttgaagcata cggaaaccag acagaagtag cacgcagact gaaatgtagt
      841 cgcggcacgg taagaaaata cgttgatgat aaagacggga aaatgcacgc catcgtcaac
      901 gacgttctta tggttcatcg cggatggagt gaaagagatg cgctattacg aaagaattga
      961 tggcagcaaa taccgaaata tttgggtagt tggcgatctg cacggatgct acacgaacct
     1021 gatgaaaaaa ctggagacga taggattcga caccaaaaaa gacctgctta tctcggttgg
     1081 cgatttggtc gatcgcggta cagagaacgt cgaatgtctg gaattaatca cattcccctg
     1141 gttcagagct gtacgtggaa accatgagca aatgatgatt gatggcttat cagagcgtgg
     1201 aaacgtcaat cactggctgc ttaatggcgg tggctggttc tttaatctcg attacgacaa
     1261 agaaattctg gctaaagctc ttgcccataa agcaggtgaa cttccgttaa tcatcgaact
     1321 ggtgagcaaa gataaaaaat atgtcatctg ccacgccgat tatccttgtg acgaatacga
     1381 gtttggaaag ccagttgatc atcagcagtt aatttggaac cgcgaacgaa tcagcaactc
     1441 acaagacggg atcgtgaaag aaatcaaagg agcggacatg ttcatctttg gtcatacgcc
     1501 agcagtgaaa ccactcaagt ttgccaacca gatgtatatc gataccggcg cagtgttctg
     1561 cggaaacctc acattgattc atgtacaggg agaaggcgca tgagactcga aagcgtagct
     1621 aaatttcatt cgccaaaaag cccgatgatg agcgactcac cacgggctac ggcttctgac
     1681 tctctttccg gtactgatgt gatggctgct atggggatgg cgcaatcaca agccggattc
     1741 ggaatggctg cattctgcgg taagcatgaa ctcagccaga acgacaaaca aaaggctatc
     1801 aactatctga tgcaatttgc acacaaggta tcggggaaat accgtggcgt ggcaaagctt
     1861 gaaggaaata ctaaggcaaa ggtactgcaa gtgctcgcaa cattcgctta tgcggattat
     1921 tgccgtagtg ccgcgacgcc gggcgcaaga tgcagagatt gtcacggtac aggccgtgcg
     1981 gttgatatag caaaaacaga gcagtggggg agagttgttg agaaagagtg cggaagatgc
     2041 aaaggcgtcg gctattcaag gatgccagca agtgccgcat atcgcgctgt aacgatgcta
     2101 atcccaaacc ttactcaacc cacctggtca cgcactgtta agccgctgta tgacgctctg
     2161 gtggtgcaat gccacaagga agagtcaatc gcagacaaca ttttgaacgc ggtcacacgt
     2221 tagcagcatg attgccacgg atggcaacat attaacggca tgatattgac ttattgaata
     2281 aagttgggta aatttgaccc aacgatgggt taattcgctc gttgtggtat tgagataaaa
     2341 agaggcggcg cttactaccg attccgccca gttggtcact tcgacgtatc gtctggaact
     2401 ccaaccatcg caggctgaga ggtctgcaaa atgcaatccc gaaacagttc gcaggtaata
     2461 gttagagcct gcataacggt ttcgggattt tttatatctg tgtaacaggt aagagcattc
     2521 tcccttatgg ggcttggctt aaatgcaccg agtgctctta tcgttgtggc agcacaacga
     2581 tagttttcgt cagagttggc gactttgcgg ttttttagaa actgaccaca aagataaatg
     2641 caaacgatga tgttgttctg atggcggcgt aatagcctgt aagtcagcaa ggtcttccga
     2701 ctccttgtaa acaaattcgg cgcactggcc cggtgtgatt aataatgggc acacaacagg
     2761 taagagcatt aaagaactgg caaagagctt aacggtctgc gaaagcattt cttagtggca
     2821 caactggccg gtacaactga gtgctctttc cggtgtggtg aatgcgcagg ctgatgcgcg
     2881 caggagagct tcggaagaac aaggtgcctg tatacaagcc ggagatcagc gccggccacc
     2941 acagccaaat ccacccagag caaaaccgtt gttcatcctt accattcact cagtattttg
     3001 ggctacacca tcagcccatt ttttaaagcg tacttccacc aagaaccaga cctaaccaac
     3061 tcattgctga cactctgtgg atacggttgt ctagtacgct ttaaaaaaga aaacccagca
     3121 tcaatggctg ggcttcgtga tatgagcggc atgtattgtt ggcgcaatcc acgcctgatt
     3181 tgctcatgaa tgcggtcacg aacaaacccg ttacaaatca accgtaaccc ggatttgttc
     3241 aagcgaccat atccataatt cctaatttga acagatcccc ttctgggggt aagacatgaa
     3301 gatgccagaa aaacatgacc tgttagccgc tattctcgcg gcaaaggaac aaggcatcgg
     3361 ggcaatcctt gcgtttgcaa tggcgtacct tcgcggcaga tataatggcg gtgcgtttac
     3421 aaaaacagta atcgacgcaa cgatgtgtgc cattatcgcc tggttcattc gtgaccttct
     3481 cgacttcgcc ggactaagta gcaatctcgc ttatataacg agcgtgttca tcggctacat
     3541 cggtactgac tcgattggtt cgcttatcaa acgcttcgct gctaaaaaag ccggagtaga
     3601 agatggtgga aatcaataat caacgcaagg cgttcctcga tatgctggcg tggtcagagg
     3661 gaacagataa tggacggcag aaaaccagaa atcatggtta tgacgtcatt gttggcggag
     3721 agctattcac tgattactcc gatcatcctc gcaaacttgt cacgctaaac ccaaaactca
     3781 aatcaacagc cgccggacgt taccagcttc tttcccgttg gtgggatgcc tatcgtaagc
     3841 agcttggcct gaaagacttc tctcccaaaa gccaggacgc tgttgcgctg cagcagatta
     3901 aagagcgtgg cgctttgccg atgattgatc gcggtgatat tcgtcaggca atcgaccgtt
     3961 gcagcaatat ctgggcttca ctgccgggcg ctggttatgg tcagttcgag cataaggctg
     4021 acagcctgat tgcaaaattc aaagaagcgg gcggaacggt cagagagatt gaggtatgag
     4081 cagagtcacc gcgattatct ccgctctggt tatctgcatc atcgtctgcc tgtcatgggc
     4141 tgttaatcat taccgtgata acgccatgac ctacaaagag cagcgcgata aagccacatc
     4201 catcatcgct gatatgcaga agcgtcaacg tgatgtagca gaactcgacg ccagatacac
     4261 aaaggagctt gctgatgcta acgctactat cgaaagcctt cgtgctgatg tttctgctgg
     4321 tcgtaagcgc ctgcaagtcg ccgccacctg tgcaaagtca acgaccggag ccagcagcat
     4381 gggcgatgga gaaagcccaa gacttacagc agatgctgaa ctcaattatt accgtctccg
     4441 aagtggaatc gacaggataa ccgcgcaggt taactacctg caggagtaca tcaggactca
     4501 gtgcctgaaa taattttttt gcaaatcaca aagtccattt aatgagcctc gcgatgcggg
     4561 gcttttttta catctgaatt tcacagcgca tctcacgcgc atattacatc acccgagcct
     4621 ttcagaaagt tgagcctgag aactgccgta tatggtggcg accatctcgg ggcggctttt
     4681 ctgtgagaca ggctcacttt ctaaaaggta aagacgctat gaatcatcaa ttggctaatc
     4741 tcgatttccg ggacatggtg gttgtttctg gtgatcgcgt gatcacaacc tcccgcaagg
     4801 tagcagctta cttcgacaag cagcatcacc acatcattca gaaaatcgaa aagctagact
     4861 gttcggatga atttctaacc agcaactttt cgcgggttac ctatgaacac aagggtaatc
     4921 agtatgttga atatgaaatt tccaaagacg gtgcgatgta catcatcatg tcgtttaccg
     4981 gcaaaaaagc tgccgccatc aaagaggcgt ttatcaaagc atttaattgg atgcgtgaca
     5041 ggctgatgga gatggctcac tcataccaaa gagagcacaa cgagttaatg ctggagttca
     5101 tgaaggaaaa ggatgttgcc agtatgtcag gacgcttgct gaaccgctgg ggcaggatca
     5161 aaaaaccgca actcatagca agaatcaaaa ggcttgagca gcaggcgcaa atatcgatcc
     5221 ccggactgcc aaagtgacca ttccaaagcc catctacggg tgggcttgat aatgaaaccg
     5281 tgatttacat ccccacaatc cgggtatgta aaagatagtt caggcgagaa cagatttaac
     5341 taaatctgtg caccaccagt tacggcagta ccgcgaaaca acccaagcca gtaagtgggg
     5401 aaataacact ggcagccact gaaagatgaa cctccagcct tatggcaaaa aagattcttt
     5461 gtggtggcga actgatggaa agacatccta atcaagcaac cactctacag ggtcataatt
     5521 atgaacgacc agcaaatcga aaaagaaatc gttgagaaag gcaaaacggc accgcgagtt
     5581 acgccagacc atatcgaagg aattattgct caggaggcat atttcacagc agaggatggt
     5641 gcctttggca aagccataaa agcgaaacat actggcggag aggtaaacta ccagtcgcac
     5701 gaatcacttt ctctgctgac gttctgcgtc ctggtgctgc gcaacggctt caccgtcacc
     5761 ggagagagtg cctgtgcaag tcaggaaaat tttgatgcag aaattggtcg gaagattgcc
     5821 cggcagaatg ctgtaaacaa aatctggatg ctcgaaggtt acttgctgaa gcagaagcta
     5881 agcgaacagt agttattaca aaagccattc cttactgagt ggctttgata atggcttata
     5941 ccctacacgg gataacttaa ctgatatccc ttttaacgga taaacggagc caacaatggc
     6001 agagattatt cccatgactg aagaacagaa attccagtta gagatttaca aactggtcat
     6061 gaaccagaac gcagccgcgg aggaagcatt tcagttcatt ggcactgacg agctgaagct
     6121 tgagctattc aaaattcact tccagtcagg tggcgctaat tcagatatca cgacccgcac
     6181 aatcgaagcg gtgcgtaaat cgaaggaagc gttagacctg ttcactaccg gagcgtaaac
     6241 atggcaactc aaggtttcga caacccatcc aaattccgcg atgaatggga taagcaagca
     6301 gaagggaaat aatcaatatg gcgactgaga aaaagaaagg tggtcgcccc tctgattata
     6361 tgccggaggt ggctaatgac atttgcgcat tgctttcctc cggtgagagt ctgcgcaaag
     6421 tttgcgaacg cccaggaatg ccgagcaaaa catcagtttt tcgctggctg gctgaacatc
     6481 aggagtttcg tgaccagtac gcgaaggcaa cagagactcg ggccgactct attttcgaag
     6541 agatattcga aattgctgac gacgtaatcc ctgatgccgc cgaggtggca aaggcaagac
     6601 ttcgcgttga tacccgcaaa tgggcgctgg cccgaatgaa tccccgtaag tatggcgaca
     6661 aggtaactaa cgagcttgtc ggcaaagacg gcggcgcaat ccagattgaa acatcaccga
     6721 tgagcactct attcggaaaa tgacctcgat taatcctatc tttgaaccgt tcattgaggc
     6781 gcatcgctac aaagtcgcca aaggcggtcg aggtagcggt aagtcatggg caattgcgag
     6841 gcttcttgtt gaagcggcgc gtcggcagcc tgtgcgtatt ctttgcgctc gtgagctgca
     6901 aaacagtatc agcgattcgg taatcaggtt gcttgaagac accatcgagc gtgaagggta
     6961 ttcggctgag tttgaaattc agcgttcaat gattcgtcat ctcggaacga atgctgaatt
     7021 catgttctac ggcatcaaaa acaacccgac gaagattaaa tcgctcgaag gcattgatat
     7081 ctgctgggtg gaagaagcgg aagcggtaac gaaggaatcg tgggatatcc tgattccaac
     7141 catccgtaag ccgttctctg aaatatgggt aagtttcaac ccgaaaaaca tcctcgacga
     7201 tacctatcag cgattcgtcg taaatcctcc cgatgatatt tgcctgctga cggtgaacta
     7261 caccgacaac ccgcattttc ctgaagtcct ccgtctggag atggaagagt gtaaacgcag
     7321 aaatccgaca ctgtatcgtc acatctggct tggtgagccg gtaagcgcaa gtgatatggc
     7381 aatcatcaaa cgtgaatggc ttgaagccgc aaccgatgcg cacacgaaac tcgggtggaa
     7441 agcgaaaggt gcggttgttt ctgcgcatga cccctcagat acagggccag atgctaaagg
     7501 ttatgcatcg cgtcacggtt cggtagttaa gcgcattgcc gaaggtctgc tgatggacat
     7561 caacgagggt gctgactggg ctacttcgct ggcgattgaa gacggcgctg accattacct
     7621 gtgggacggt gatggcgtcg gtgcgggcct acgcagacag acaacggaag cattctccgg
     7681 caagaaaatt accgccacga tgttcaaggg cagcgaatcg ccattcgatg aagatgggcc
     7741 ttatcaggcc ggagcatggg ctgatgaagt cgtacagggc gacaacgttc gcactattgg
     7801 cgatgtattc cgcaataagc gagcgcaatt ctattacgcg ctggctgaca ggctgtatct
     7861 gacatatcgg gcggttgtcc acggtgagta tgcagacccc gacgacatgc tgagcttcga
     7921 caaagaagcg ataggcgaga agatgctgga gaagctgttt gcagaactga cgcagattca
     7981 gcgcaaattc aataataacg ggaagctgga gcttatgact aaggtcgaaa tgaagcagaa
     8041 gctcggtatc ccatctccta acctggctga tgcgctgatg atgtgtatgc attgccctgc
     8101 attggtccgc gaagaaacag aaatatacgt tccctcatcc tccggttggt aaacatggca
     8161 gagacattag agaaaaaaca tgagcggatc atgctcaggt ttgatcgcgc ctattctcca
     8221 cagcaggaag tgcgcgaaaa gtgcattgaa gctacgaggt ttgctcgtgt ccccggaggt
     8281 caatgggaag gagcaacggc ggctggaact aagcttgatg agcagttcga gaagtatcct
     8341 aagtttgaaa tcaataaggt agcaactgaa cttaaccgca tcattgcaga ataccgcaat
     8401 aacagaatca ctgttaagtt tcgtcctggt gacagagagg caagcgaaga gttagccaat
     8461 aaattaaatg gtctgttccg tgctgactac gaagaaaccg atggcggtga ggcttgcgat
     8521 aatgcatttg acgacgctgc tactggtggt ttcggttgct tccgtttgac gtcgatgctg
     8581 gtcaatgaat acgaccccat ggacgatcgt cagcgtattg ctattgaacc aatatacgac
     8641 ccgtcgcgct ctgtgtggtt tgaccctgac gctaagaagt acgacaaatc tgacgcgttg
     8701 tgggcgttct gcatgtattc gttgtcacct gaaaaatatg aggctgaata cagaaagaaa
     8761 cctcctgctt ctctggatgt aacgtctatg accagttggg aatatgactg gtttgatgaa
     8821 gatgttattt acatagcgaa gtattacgaa gttcgtaaag agtctgttgt cgtcatcagt
     8881 tatcgacatc caatcactgg agagattgca acatacgaca gtgatcaggt tgaagatatt
     8941 gaagatgaac tggcaatagc tggatttcag gaagtggcaa ggcgctcagt gaagcgccgt
     9001 cgtgtgtatg tatccgtagt ggatggtgat ggtttccttg agaaacctcg acgtattcct
     9061 ggtgagcata tccccctcat cccggtttat ggaaaacgct ggttcattga tgacattgag
     9121 cgtgtcgaag gacatattgc aaaagcaatg gatccacagc gtttgtataa ccttcaggta
     9181 tcaatgctgg ctgatactgc agcgcaagac cccggtcaga tccctatagt tggcatggag
     9241 caaattcgtg gacttgagaa gcactgggag gctcgcaaca agaaacgccc agcgttcttg
     9301 ccgttgcgcg aagtgagaga taaatctggc aacattatcg ctggagctac cccggcagga
     9361 tatacacagc ctgcggttat gaatcaggca ttggctgcat tactacagca aaccagtgct
     9421 gatattcagg aggttacagg cggcagtcag gccatgcagc agatgccaag taatattgct
     9481 caggaaacgg ttaacaactt gatgaacaga gcagatatgg cttcgtttat ctatctggac
     9541 aatatggcga aaagtcttaa acgcgctggt gaagtatggc tgtcaatggc gcgtgaagtg
     9601 tacggttcag agcgtgaagt gcgcatcgtt aacgaagatg gaagtgatga tatcgctgtc
     9661 ctgagcgcac aggttgttga caggcaaaca ggggctgttg ttgcgttaaa tgacctttct
     9721 gtcggtcgat acgatgtgac ggttgatgtt ggaccaagct acacagcacg acgtgatgca
     9781 acggtttctg tactgacaaa tgtccttagc tctatgcttc caacagaccc aatgcgcccg
     9841 gcaattcagg gtattattct ggacaatatc gatggcgaag gccttgatga cttcaaagag
     9901 tacaaccgaa accaactgct gatatctggt attgcaaaac cacgcaatga gaaagagcag
     9961 cagattgttc aacaggcgca aatggcagca caaagccagc caaatcctga aatggttctc
    10021 gctcaggcgc aaatggtagc agcgcaggca gaagcgcaaa aagcaactaa cgaaactgct
    10081 caaactcaaa tcaaagcatt tactgcccag caggatgcga tggagagtca ggcaaacact
    10141 gtctataaac tggctcaagc cagaaacatc gatgacaaag cagtgatgga ggcaatacgc
    10201 cttctgaaag atgtcgccga gtcacaacaa cagcaattcc agtcaccacc acagtcaccg
    10261 gcagacttaa tgccgagtta accaggagta atcaatggaa aacgaactga tcatcgacgg
    10321 tcaggttatt gacctgtctg aaacacagga aaatgcagaa gaaaccatca tccaaacaga
    10381 gtcacagcct gagaatgaaa gccaggatga caacggaaaa gagatggcaa ctgatcctga
    10441 aaaaaccgaa gagacaccag aagattacgc cttgcgtatt ggtgatgaag aaattcagct
    10501 taacgctgac gataatgatc acattgacgg gcaacctgca ccgcaatggg tgaaagatct
    10561 tcgcaaaggc ttcaaagaaa cacagaaaga aaaccgtgag ttgcgacgcc agcttgagga
    10621 agcattagcc aagcatgcgg aacatcagca accacaacca gacgctattc caccaaaacc
    10681 gactcttgag tcgtgtgatt atgacgaaca ggcgtttgaa caggcattga ctgattggca
    10741 tgagaaaaaa ggccgtgtcg aacagcagca gcaacaaaaa ctacgtcagc aacaggaata
    10801 ccagcagcgt ttccagcaaa gggtagaagc gcataaacaa cgggcagcca aacttcctgt
    10861 gaaagattat caggaaatgg aagccattgt tcttagtgag ctaccaccaa ttcagcagga
    10921 aatcatcatt cactgtgcag acgaaggctc tgaactactc gcctatggct taggtaagag
    10981 ccagcaatta cgccagcgtg tagccgctga gacagatcca attcgcgcag cattcctctt
    11041 ggggcagatt agcaaacagg taagccttgc tccaaaacca aagaaagcca tcaagccaga
    11101 gccggaagta cgtggtggcg gtgctgatgc gaaacaagac gaattcaaca aattatgccc
    11161 cggcgcaaaa atcgaataag gaaaagataa atgcctaaca atctcgacag taacgtcagt
    11221 caaatcgttc tgaaaaaatt ccttccgggt tttatgtcag atttagttct ggcgaaaacc
    11281 gtagaccgtc agttgctggc aggtgaaatc aactccagca ctggcgatag cgttagcttt
    11341 aaacgtccgc atcaattctc atccctccgt actcccactg gtgatatttc agggcaaaat
    11401 aaaaacaacc tgatctcagg taaagctacg gggcgtgtag gtaactacat cactgttgct
    11461 gttgaatatc agcaactgga ggaagcgatc aagcttaacc agctggaaga aattctcgcg
    11521 ccggttcgcc agcgaatcgt taccgacctt gaaacagagc ttgctcactt catgatgaat
    11581 aacggtgcgt tgtcacttgg tagccccaat actccaatca ccaaatggtc tgatgttgcg
    11641 cagacggcat ctttcctgaa agacctcggc gttaatgaag gtgaaaacta tgctgtaatg
    11701 gatccatggt ctgcacagcg acttgctgat gcgcagactg gtttgcatgc ttcagatcaa
    11761 ttggttcgta ctgcatggga gaacgcacag atcccaacca attttggcgg cattcgcgca
    11821 ctgatgtcta atgggcttgc ctctcgtacg cagggggcat ttggcggaac actgacagtc
    11881 aaaacacagc caactgttac ctataacgca gttaaagact cataccagtt cactgtaaca
    11941 ttgaccggag cgacagccag cgttacaggt tttctgaaag ctggtgatca ggtcaaattc
    12001 accaatacct actggctgca acagcagacc aaacaggcgt tgtataacgg agccacacca
    12061 attagcttca ctgcaacggt tactgctgat gctaattcag acagcagtgg cgatgtgacg
    12121 gttacgcttt ctggtgttcc gatttatgac actacaaacc cgcagtacaa ctctgtaagt
    12181 cgtcaggtag aggcaggcga tgccgtatct gtagtaggca ctgctagcca gacaatgaag
    12241 ccaaacctgt tctataacaa gttcttctgt ggacttggat ctatcccact gccgaaactg
    12301 cacagtattg attctgctgt tgcaacatat gaaggtttct ccatccgcgt acataaatac
    12361 gcagatggcg atgccaacgt gcaaaaaatg cgcttcgact tactgcctgc atatgtgtgc
    12421 tttaacccac acatgggcgg tcagttcttc ggtaatccgt aataacaagg ggcttacgcc
    12481 ccttttatgt tttaaggaaa caatatggat cggatgagtg tattccttgc cgcagataac
    12541 gaatccgggc atgtacaggc cgttatcgca gaaaaagact tccagttttt cgaaaggttg
    12601 ggctttgttg cctcagttga tgaattgaaa ccgaccagta agcgaggtcg taaggcggcg
    12661 gacaatggca acagtactga caaagggtga gatcgtcctt tttgcgcttc gtaagtttgc
    12721 tattgcttct aatgcatcgc ttactgatgt tgagccgcaa tcaattgaag atggtgtaaa
    12781 tgatctggaa gatatgatgt ccgagtggat gattaacccc ggcgacattg gttacgcttt
    12841 cgcaactgga gatgagcagc cattaccaga tgatgagtca ggtcttccaa gaaaatacaa
    12901 acacgcagta ggctatcagt tattgctgag aatgctatct gattacagcc ttgaaccaac
    12961 tccgcaagtt ctcagtaacg cccaacgctc atatgatgcc ttgatgaccg acactctggt
    13021 tgttccttca atgcgacgac gtggagattt tcctgtaggg cagggtaata aatatgacgt
    13081 gttcacatct gaccgatatt atccaggcga tctccctctg attgatggcg atatcccaaa
    13141 cgcataggtg aataaatgcc tattcagcaa cttccgctta tgaaaggtgt cggcaaagac
    13201 tttagaaacg ccgactatat cgactatctg ccagtgaata tgttggctac acccaaagaa
    13261 atcctgaaca gcagcggata tcttcgctca ttcccgggca ttgccaaacg ttctgatgtg
    13321 aacggcgtat cgcgaggcgt cgagtacaac atggcacaga atgctgttta tcgcgtgtgt
    13381 ggtggcaaac tgtataaggg cgaaagtgag gtcggtgatg ttgccggaag tggtcgcgta
    13441 tcaatggcgc atggtcgaac atcacaggcg gtaggcgtta acgggcaact ggtcgaatac
    13501 cgctatgatg gcacggttaa aaccgtctca aactggcctg cagacagcgg gtttatgcag
    13561 tatgagttag gttctgttcg cgacattacg cgtttacgtg ggcgttatgc gtggtcaaaa
    13621 gacggcactg attcatggtt tatcactgac cctgaagacg aatcgcatcc tgaccgctac
    13681 agcgcacaat atcgagccga gtctcagcct gacggcatca tcggcatcgg aacatggcga
    13741 gacttcatcg tctgctttgg ttcatcgacg attgaatatt tctccctgac gggggcaacc
    13801 accgttggtg ccgctttgta tgtcgcacag ccatcgctga tggtgcagaa aggcattgcc
    13861 gggacttact gcaaaacgcc gttcgccgat tcgtatgcgt tcatcagcaa tccggcaaca
    13921 ggtgcgccgt ctgtgtatat catcggctcc ggtcaggtat caccaatcgc cagcgcgagc
    13981 attgagaaaa ttcttcgctc ctacactgct gatgaactgg ctgatggtgt gatggaatcg
    14041 ctgcgatttg atgcgcatga actgctgatt atccaccttc cgcgccacgt cctcgtgtac
    14101 gacgcatctt caagcgccaa tggtccgcaa tggtgtgtgt tgaaaacagg cctgtatgac
    14161 gatgtgtacc gcgctatcga cttcatttac gaaggaaatc agataacgtg cggcgataag
    14221 ctggaatcgg ttaccgggaa attgcagttc gatatcagca gccagtatgg gctacagcaa
    14281 gaacacctgc tgtttactcc gttgttcaaa gcggataacg ccagatgctt tgatctggag
    14341 gtggaatcat cgacgggtgt tgctcagtac gctgaccgcc tgttcctctc tgcaaccact
    14401 gacggcataa attacgggcg tgagcagatg attgagcaga atgaaccgtt cgtttacgac
    14461 aaacgcgttt tgtggaagcg agtagggcgc atcaggaaaa atgttggctt caaactgcgc
    14521 gttatcacta agtcgcctgt cactctgtct ggcgctcaga taaggattga gtaatggcgg
    14581 attcgaatct caatgtgccg gtaatcattc aggctacacg gctcgacaca tcagtccttc
    14641 cacgcaatat cttctcgcag tcgtatctgc tttacgttat cgcacagggt actgatgttg
    14701 gtaatgtggc taacaaggcc aacgaggccg gacagggcgc ttatgacgca caagtcagga
    14761 acgatgagca ggatgtgatt ctcgctgacc atgagcagcg aatttctgct gcggaagcaa
    14821 cgcttgttaa tcatgaggag cgaatcagcc aggcagaatc aactcttcag gaacatgaaa
    14881 cgcgaatcgc tcagaatgaa agcgatattg cgtcgcttga taccagagtt cagtcgctgg
    14941 aatcgcaggt ttcagaccat gaatcgcgca ttgatgctct ggagtatgcc actactcgca
    15001 agaagtcaga ggttgtttac tctggcgtat ctgtaaccat cccgacagcg ccgaccaacc
    15061 ttgttagcct gctgaaaacg ctcacgccgt catcaggctc gttggcacca ttcttcgaca
    15121 ccgttaacaa caagatggtt gtgttcaacg agaacaaaac cttgttcttc aagctgtcga
    15181 tcgtcgggac gtggcccagc ggaaccgcca acaggtcaat gcaactaaca ttttccggct
    15241 ctgttcctga cacactggta agcagtcgca actcggcgac aacgaccgat aacatcctgt
    15301 tagctacgtt cttcagcgtg gataaagacg gctttcttgc cacaaatggc agcacgttaa
    15361 ccattcagtc aaatggtgcg gcgtttactg ccacaaccat caagataatc gcggagcagt
    15421 aatgattcag ttcaaaccaa cgcgaaacat cgacctgata gaagcagtcg gaaatcaccc
    15481 tgacattatt gccggaagca acaacggtga tggatacgac tacaagcctg aatgccgtta
    15541 ctttgaggtt aacgtgtacg gtcagtttgg cggcattgtt tactatcagg aaattcagcc
    15601 gcttacattc gattgccacg ccatgtacct gccagaggtt cgtggattca gcaaggaaat
    15661 cgggctggcg ttctggcgat acattctgac taacaccacc gttcagtgcg tcacatcgtt
    15721 cgctgcgcgc aaattccgcc acggtcagat gtactgcgca atgattggcc ttaagcgtgt
    15781 aggaaccatc aagaaatact tcaaaggcgt ggatgacgtg acgttttaca gcgccacacg
    15841 cgaagaacta atcgacttcc tgaatcacgg gagatagcta tgttatatgc atttaagctg
    15901 ggcagaaaac tgcgcggcga ggaaccttat tgccctgaaa aaggcgggaa aggtggcagc
    15961 tctgataaaa gcgcaaagta tgcagcagaa gctcagaagt atgccgcaga cctgcaaaat
    16021 cagcagtggc agacgatcat gaaaaacctt gctccgttca cgccgcttgc ggagcagtat
    16081 gttaaccagc ttcagaatct ttccagttta gaaggtcagg ggcaggcact taatcagtat
    16141 tacaactctc agcagtataa agaccttgca ggtcaggctc gttaccagag tcttgctgct
    16201 gcggaggcga cgggtggact tggttcgaca gccacaagca atcaactggc tacgatcgcg
    16261 ccgacactcg gtcagtcttg gttatcaaat cagatgagca attacaacaa tctggcaaac
    16321 gttgggcttg gtgcactgca aggtcaggca aacgccgggc agacgtacgc caacaacatg
    16381 agcagcattg cacagcaaag cgcagcactt gccgctgcta atgccaataa accatcaagt
    16441 cttcagactg caattagcgg cggcacatct ggtgcgattg ccggtgcagg tcttgccagc
    16501 cttttgggaa catcaacgcc ttggggcgct ggcattggtg ctggtatcgg attgcttggc
    16561 tcgttgtttt aaggggtaat catggctact tggcaaggaa caaacggcgg attgttggct
    16621 ggtatcggcg gcgtcaactc aaacgctccg agcgtaaatg acatcggcaa tacgcttcag
    16681 cttatcaggc agaacaatga tattgatcgt tcaggcgcta acaatgttgg gctgactgct
    16741 ttgcaaggcc tttcaggtat tgcaggggtg tttcagcagg aaaagtaggc tcagcggcag
    16801 aaagaatttc agcaggcgta cgctaatgct tatgcgtctg gtgatcgcgg tgctttgcgt
    16861 cagttggcta ctcaatatcc agaccagatt gaatccgttc gtaaaggcat gggattcatt
    16921 gatgaagacc agcgtaattc tatcggcacc ttagcggctg gcgcacgcct tgcgtcatcg
    16981 tctccagaag caatgcaatc atggctgcaa aacaacgcca aggaactgac tcgcgtcggt
    17041 gttgacccta acagcgttgc tcagatgtat cagcagaacc cttcaggatt tggtgagttt
    17101 gttgatcacc ttggaatggc tgctcttggt ccaattgatt acttcaatgt tcaggacaag
    17161 atggctggtc gtgaaattga ccgaggcaga ctggcagaga caatccgcag caatcaggct
    17221 ggagaagcac ttcaggcgag agggcaaaac ctttcctatc agtcagcaat gactgggcac
    17281 aatatcgcag cacaacgctt ggctctggat cagcaagagt tcgggtttaa gatgcagcaa
    17341 gcgcaggaaa aggctcagca gttgattagc gaagcaccta agctgtcagt aaacatggaa
    17401 aaaggcatcg agacggctgt aaacaatgct acagcatcat caaactcagc caattctatg
    17461 agtgcgcttg ctcaacagtt cagagcagaa aaaccaacga caggtttgtt cggtaacgca
    17521 cagaacatgt tcgcaaaact taccggaagc gatacaacat tgcgtgattt gcgcattcgc
    17581 caaaatgccc ttgttaacag tcaggttctt aaattcctac ctcccggccc agcaacggat
    17641 aaagacgttg agatcgttcg acagggtgcg ccaactgaca tggataaccc tgagacggtc
    17701 gcaagatggc ttgatgcaat ggcaaacctt gagcgacgaa acgcgcagtt taatgagttt
    17761 aaagccgagt ggatgagcgc gaatggcaac cctggacaat cgcgtaatgg cggtcagata
    17821 ttggggttgg atgttaaaaa aggtgaatca ttggggagtg ccgttaagcg gtatatgtca
    17881 atgaatactg acgcagcgcc agcacaagat tcgacacctt caggagaacc acggaatcag
    17941 gttggatcat atacctcaaa atcaggcatt caatttacgg tggaatgatg aaagtaactg
    18001 caaacggtaa gacatttacc tttcctgatg gtacgagcac cgaagatatt ggcaccgcca
    18061 ttgatgagta ttttgctggt caggctgttc agcaacaaac agttaatcag gccaataatg
    18121 caccaacacg ggacgaacca tcattgatgc aacaagctgg cgattggctc actggtggtc
    18181 aaagtgcagg gcaaattgca gaacaggctg gtcgtggtct ggtaaacata ccatttgacg
    18241 tattgcaggg tggcgcaagt ctgattaatg caatcagtca ggggcttggt gggccaaaag
    18301 ttttggatga tgtttatcgt ccagtagaca aaccgacaga cccatacgca caagccggtg
    18361 aaacaattgg tgggtattta gttccaggag ttggaacggc aggaagcatg gctattggat
    18421 cgctggcaga ggccgcaaat cagaaaggcg atttcgcaca aaatgcagct aaaaatgccg
    18481 gagttaacct tgccgctcag ggtgttcttt ccgcagcagc aaagggaata gggcgtggaa
    18541 taacggctat aaaaggtgat attgcgccag aagtggcgaa gaaaattgcc acatcagaat
    18601 cgatgggcgt gacaccaatg acatctgatg ttatcccgcc gaaaaatgct ttcactcgcg
    18661 gccttactca ggatgccgag ggggctttgc tcgggacggg ctcaaagcga gcagagcaat
    18721 atgcaacgcg tagtaagctg gtaagcaatt attttgaccg ttttggtgag tacaaccctg
    18781 atgatgtggt gaaatctctg accaccacgt taagggggcg gaaggatgct gctggcgctg
    18841 ttatcaatga cgtcaccaat aaaatgggta atgccgcagt tgatactaca aataccatga
    18901 atgctctgaa tacagcgatc gcaagacagg aacggcttgg gacttcagcc aatcaaagcc
    18961 tgcttacatc cttgcgtaac ctgcgtgaag aattagcaaa ccctgcaact gatttggatg
    19021 ttacgtttga tctcttgcgt cagcacagaa cagcatttag atctaatgtt cagggagatg
    19081 ctatggtctt ccccaaccag gcaaaagcag ctaccaatat ggtagagaat gcaatgtcaa
    19141 aagaccttcg taacgcagtt gcaaaaaacc tcggtgcgtc agacgcagca aaatacctta
    19201 aagcaaattc cgattatgca aacgtttata ataaggtgct taataaaaac attgctaaca
    19261 agctcaacaa ggcaagcagt gaagccagtc ctgaacttat aaatactgtt gtattaagca
    19321 gaaaaccatc tgacgtgaaa cgaatctgga gcgcattgga tgataaaggg aaagatgcta
    19381 tgcgtgcagc ttacgtcagc aaaatagcgg aaaaggccgg tgactctcca gccaagttca
    19441 tcactgaagt taataagctg aaatctcagt caggtggtga aatttacaac actatttttt
    19501 ctggaaagca catgaaagag cttgattctc ttcatgaagt tctacagaaa acagcaaggt
    19561 cagacaccgc aaatgtagta actcagactg ggcaatcgca agccaacagg ataaggacga
    19621 ttggcgcaac tgcgactctt ggcgtatcaa tggggcttga ggctggtttc ggtgcaatga
    19681 tgcgcttgta tgagtccaaa gcagcaagga atgctctctt acgtttggca aacaccaaag
    19741 caggaacacc agcctatgaa agagcgctaa ataatgctgc aaatgcgata cgccctatac
    19801 tctcaagcca aattacagca gaacagcaat aaaagataag atgtaactac ctgatattac
    19861 tgctactgtt gcatgttacc gtgtttccaa atcctgaatt gcagtttgta tatgtgtcaa
    19921 cgcgtgttgg gtaaggttga gttataacag gctggcgcgc tttttgctcg atcgcttgca
    19981 ttgtgtttac agcctgataa ttcaataaag cctgctggaa tgcttggctt tgtgctattt
    20041 gttgggcttg ttcttggctt tgtaattgaa cataaagatt ctgaagctca agtcttgcct
    20101 gtgcgtcact tatcttgcct tcatcgacac ctttcccgag catctttgca gcaaggacat
    20161 acagcttagg tgttggtgct gatgccatgc gtgagtcgtt cttcacactg gcatcaaggc
    20221 aattagccat atcgctaagc ttttgatagc gttgttcgca acttgcttga tagtcactta
    20281 cttttgcgca tccaaccagc agaagcggga taattaacag tgattttttc atatggttaa
    20341 ctctccttag tttttcacag gatatcatga aggcaatgcc attttagccg gaaactagat
    20401 ttctatgttt cctttttatt attgctatac atggtcttaa gcgtttcaaa aaccattttc
    20461 ttaaccatat cagattgttg ttctgccata cgctctgcat catcaatgta aacggatgca
    20521 gagctttgtt tagccaacga ttcttcaatc gctgcaatta tctctgagtt cagcgacctg
    20581 ttattcatct tcgcgcgctg tttaattttc gcgtggagtt catgcggaag tctcaagtga
    20641 aactgcgcct cgtcgtattt gctgtacatc cttgatgcct caccagttgg gtggaatggc
    20701 atcgtaacct actggataaa tactcaatag taccatttcg gtatgcaatc acatcatggt
    20761 tgcatcatat cattcttctg gagcaatgaa atgtcagata tcaccgcaaa tgttgtggtc
    20821 agcatgcctt cgcaactctt cactatgtct cgctctttta aagccgtagc caatggaaaa
    20881 atttatatcg gtaaaattga cactgacccg gtaaatccag aaaaccagat tcaggtttat
    20941 gtggaaaacg aagacggttc tcaccttcct gtttcgcaac caatcatcat taacgctgct
    21001 ggttaccctg tatataacgg acagattgcc aagttcgtaa ctgtgcaagg ccattctatg
    21061 gctgtttatg atgcatacgg tgcgcagcag ttctattttc cgaatgtgct aaagtatgat
    21121 ccagatcagt ttaagggcaa tctcctgagc gaagatcact cacttggcga ctctctagtc
    21181 atgcataacc cacagtttgt cgatagcaca tcgaccattc tctcaaaaaa gctaactatc
    21241 gacctgagga cgcttacaga ctacgggttt gctgagggaa acaccggtgc tcagaacaaa
    21301 gctgcatttc agaaggctat tgatgatgct acgttgccaa ctgagattgt tataccagag
    21361 ggggcgttca ttgttgaccc tggtattacg attaagaata ctgtcaccat gatacgaggt
    21421 gctggcgcat atcagtccag aattttctct acgggtacag cggcaccgat aatcacgcaa
    21481 caagatgggg taattacgtt ctgcgagttc agggactttg gtcttgacgg aaatggctac
    21541 gcagctaacg gcatcagtct tactgaagcc aaccacatta aaatagaaaa tattgacgtg
    21601 gttaatacta ataacaacgc aatattagtt aacggttact ccattgacat cattgggtgc
    21661 cggttgtttc aaaacgccgg taacggtatt aatgttggtg gttactgcaa taatattaat
    21721 ataatcaaca gccgtatcta tggtaacgga gccggtggag ttttacttac gccagcttac
    21781 gctgagggcg gtatgagcgt cagagttaat ggcaacaata tagagcaaaa taagttctac
    21841 ggacttttgg cttacggggt taaaggcctt aaccttgatg ctaactattg ggaacgcaac
    21901 ggtgagattg gttacccgta tagtgtacct gagtctatta cagtacgtgc ggatatccac
    21961 cttatcgcga acaattttac attaattcct gacctttcaa aaattaatga taccgtatct
    22021 attcgcggga atcagcagac cgcgattggc tacgccagcg cgctacctaa ccaagacgga
    22081 tttattttca cgaactatgc gaaaaacctg acgattgaaa ataatcagtt attagacgca
    22141 tcgaaagtga ataatttatt agcaatgtac cacaataatc tttcgtccaa agttactgac
    22201 aggctatatc ttgccaacaa cacagtgaac tccatcggat acgttggtag ttatgacccg
    22261 gcgacacaaa accctgacac tgcgcattta atagatattg cgaacagaga gttaactgct
    22321 aactatcttg atcgaaatat gttgctctgg actgcggcat ctggaactac aggaacattg
    22381 ataaaaactc aaaacatcta tgctggtaat tattcattct tagttacgac aggagataga
    22441 gtttggggac gaacgataga cctcaacaaa tcaccagaac taaaagggaa atttgtatgg
    22501 tttggtgcgt gggtcaatga ccagggatca gcgtcaaaac ttatgtttat aattaatggt
    22561 gcaggacaaa cagactctac agcaccttta gctggtaatg ggaaatggag ctatgttagt
    22621 tgtggtgtgt atatatacga aactgataca gctataaatg ttggtataag aaattatggt
    22681 tcaggaaatg tactcataaa ctcaccttct ctatgtgcat atggaatgcc atcaaacgcg
    22741 ttgcaagttg aaaaaacaac attttatctt tcgtcagtac ctacatccgg tttctgggat
    22801 ataggtgagc gtgttatcaa tagcgcacct gcttcaggac aaccaaaggc atggacatgc
    22861 aatattccag gtgggccagg aacctattct ttcttatcag agggaaactt ttaaaagacc
    22921 tatgtattga aatgattact ttgatctggt atgtttaaaa aaaatcagta aaacacattt
    22981 tgatatggaa agaaaaacaa ataagttaga ctctattcaa gtccttagag ggatcgcggt
    23041 acttctagtc attgcgtttc actttagagt atatttaaat ggagtgtatg cccaaaagga
    23101 cttgggcgac attctttttg gtataggtga ggttggtgtt gatatattct ttgttataag
    23161 tggatttatc attacttatt cttcaatgaa taaggcaaaa aaccggccaa tggtgtttgc
    23221 tgctaagcgt ttttttaggt tgtaccctgt ttactttata atccttactc tggctatata
    23281 cctcaactat gaggcaatat atacatcatc acaaataata aagagctact tgctcatacc
    23341 aattgactat aattttatag ggccatggtc aggttatagt ataataatac ctgcatggac
    23401 actaacgtat gaaatttggt tctatgtggt tttctttttt tcattatgca tcagccataa
    23461 atatagaact gcgatcgcat catttcttct catcattatt tgcgttcttg gtcaactgta
    23521 ctttcacgga gatgtttttc taaatcctat cgcgcaaccg ccatctggtg atggaattat
    23581 tcgtaatctg gtatttgtat caaatccaat aatatatgac tttattttgg ggatgattat
    23641 tgcagaggcc tacattaggc taccttctag acttataaat aaaatgcctg ttgttcaatt
    23701 tcttaatatt acattagcga ttgccttgct ttttttatgt ctagggttaa ggaatggacc
    23761 tggaattgta gcatgggggg cttattcatt tatgattata gccccaatag tccttatttc
    23821 taagtgtagg gatgtatata gaaataaaat tctaacgtat gttggtgaaa tatcctattc
    23881 actttacatt aatcatatgg ttgtatacag tcttgctgtt ttatatctca aaccgtacgg
    23941 ggtattggat agtaaaggat ttggtgtttt tctagctatg atttgcgcta cttttattat
    24001 atcaatcata tcttataact ttattgaaaa accatcagta tccatagggc atgccatcgc
    24061 tcgtagaatg caaaaaaaat cagatgtatc ttcaattaaa actgaatccg ttagagaagc
    24121 accttgataa atagagcaat agccagatag cacctaaccg atttcgtact catagggttg
    24181 tcactttacc aaactgagag gaaatacact gataacagtt ggttatgaag tactgggtga
    24241 ggtggatgtg aatggtattt tgatgcattt cattaacctg tacgcctaag aaagcaaccc
    24301 tgaaatctaa gacacacaag ctttgcactg gattgcaagg ctttgtgcac ttcgatagtg
    24361 gttaaggtgg ttcactccac cttttcatca agccagtccg cccaccattg catcatttct
    24421 ctgcgcttat cgagatactg agcatggttg taaataccgc gcacagatcc gccgttggca
    24481 tgtgccagtt gcacttcaat agcgtcagca ggccattcgt gctcgttcat aatcgtgctg
    24541 aattcatgcc tgaatccgtg accgctttcc agaccctcat agccgatttg tttgatcaca
    24601 agtagcaccg cgttctcgca gattggcttc ttcttatcgt tgcgcccggc aaaaacaaat
    24661 tctgatactg gtttggtgat ggagcttaac gtagtgagaa gttcaaccac ctggtccgac
    24721 atcgggacaa catgaatttt gcgtcccttc atcacattgg cgtcaatggt gataatcctg
    24781 ttttcaaaat cgacgttctt ccattgcatg gaacgaagct cttttgttct tagggctgtg
    24841 tagcgtaaaa ccttggtggc aatgagcgat acgatacttc ctgaaaatgt tgccagtgct
    24901 ttattgaatg ccgggatctg gtctgcagga agaaacggga agttcttctt gcggtatccc
    24961 ctcatggcgt cagcaaggtc aggtgccggg ttatatttag ccctgccggt gacaatagcg
    25021 taacggaaaa cctcgccgca tcttctgcgg gctttatttg ctcgctccat tgcaccgcga
    25081 tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg aatttcaagg
    25141 ccgccgatga taggcaaaat gtcgtcatca aacatttttg caagttcagt cgcataccct
    25201 actgaccata cttgcttctt gtgctcgtac cattccttgt aaatcgcact aaaggaattg
    25261 ttgttagacg aagccttttt cgcctttacc ggatcgatgc caaccgagat gtctttcctc
    25321 gcagtccatg ccttatccct tgcctcctgc aaagtcatta gcggatattt tccgacggtc
    25381 aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt cccggataca
    25441 ggaacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc tttcggcttt
    25501 gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggagggtaa aattatttta
    25561 tccacttttt acccgtcatg gggtgcggct gtcaacgatc tgacgcgaac catgacgaac
    25621 tgtgaatcta cggaaggctt gatattcagg ggattttgcg gactggtacg gatgggagcg
    25681 aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta ggaggggctc
    25741 gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa tggactgcgg
    25801 ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg tttttatcat
    25861 ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta cgcttgttgc
    25921 tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg ccgccgcaga
    25981 tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag tgcagcgtgc
    26041 cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgggtg gtgtcaccca
    26101 gtgcaataag ccaggtcatc aaaataatca ccacaatctt tgccgcaccc gctgcaggaa
    26161 acatccaaac catagtggcg atcagccagc cggaaatgat cgcgttggca aacatctcgc
    26221 tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga gtttcttcat
    26281 tgaagatagg catatattca aatgcccatg cagcaatacc tgtcccgaga atattaccca
    26341 gcagcacgac gccccataat cgcataagta agccgacgtt gctcattgtc ggtttttgca
    26401 tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg gccataatga
    26461 cgataataaa accaaaggta taaccgagat tctccagtaa gaagctgcct ggcactcctt
    26521 ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac agacccgccg
    26581 caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca ccatcctggc
    26641 ggatatgctc atgaattgcc atcgcccggg aggggagtcg gtcttcatct atttctattt
    26701 ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt tgatcaattt
    26761 tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg tagcggcttt
    26821 tttgctcgca actggcggga gttactctga aaatgtagaa aaggctgcgt ttgccttttt
    26881 ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg atcaaatcag
    26941 caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca cctaaacagg
    27001 cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa aaacagggag
    27061 acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct ggtggggtgt
    27121 gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt caaccgcacc
    27181 atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt cgctgtcgcc
    27241 tggcgtgatt atgttccgca accggcgcgt aatggtttga gcaactttac tggcaacctt
    27301 gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca ggggatggtc
    27361 cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat tgatgttgca
    27421 gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag tacgcttggt
    27481 cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag cttcacgctg
    27541 cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg gctgacctgg
    27601 ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca gctgctggat
    27661 tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga agcgtacttc
    27721 cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa tccgaacgca
    27781 caagcgattc aggatgattt aaaagatatt gattctgaat aagaaacaaa taaaaaaggt
    27841 gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta aaaggagata
    27901 aaccgacaat gacccggacg taatgatgta aaaatactga tgttcgtcac tgactatttc
    27961 cgatattcag gcgttgaaaa atcaaaaaac tggaaaaata attttaaata tcatcaaaat
    28021 taacacctgg cgcagatagt tgattaattt cattttgata tttcgcctgg cgattttgaa
    28081 gtcttttttc aacgttatct tcgagatcct taaagtcttt gtgtttattt tcaggatggt
    28141 caacgtcaac tttgttagcg aataaattag aaattatttc ttttacattg tcgcgtctaa
    28201 attttgcatc gtccacttga aaattatatc ttattgctcg acctaaacgt ttaaaaactt
    28261 cgttaggttc tctcagtctt tccccgttga gctcgtttac aacctgatga tcggaacctg
    28321 ttgcaaatga ttctgccatg atagctttac tgctttcaaa ccacgctgaa gcattcctgg
    28381 ctttttcact acaatattgg gtgagactat caccattaat acgaattcct ctttgaatgt
    28441 ggtagtcgtc ctgatcgtag taaccgagag ataagtaatc atttccgttt ttatttttaa
    28501 ttacaatagc gagagagtcg ccttttttat ctttaccgta ccaggtaaat aagtccgttg
    28561 gttcgctatt cccgctctca tcaaactccg ttaccgtatg tctgagtttg tgtgatatct
    28621 tatcgaagat aaaaccagga tctccatcat atatttcttt taatgcgctg aagatcattt
    28681 ccttgttatt gatatcatga gtcttctcaa ctatgttgtc attaatgata gtacggacga
    28741 gttctttgtc tttatagttc gcaggtaatt ctattggttc ttggttgtcg ataccaatag
    28801 taatatggtc tgtatcattg gagccaatat caatataagt aactgaggaa ttattaatat
    28861 taaatctata aggatcgtcg tttactcgta tggcgttttt tataagctca agtagatgtt
    28921 taatacaggt gcttattcct tccttaccat taaaaaggtt tttgagctct tgtactaatc
    28981 cattaacttc atcattgtta ttttcattaa tggattctgg gtaagttgcg ctggtattgt
    29041 tgattgaaat agatggtagc atgattttat tccattttga ttgatttgtt acaaatatat
    29101 attacctaat gaaatagact atcaaaaaac gctcatttat ttagaaccta tctttaattt
    29161 gagtgtatga gcgcacaata gccagggggg tgagtatcct ggaaattcag aaaagtaaac
    29221 tctttgagaa ggacgagcag aacgcctgaa aatatgcctt tcccttcaga tttgtctgtg
    29281 gataccgctt attgatttga aagggcaagc tgccatcagg ctgtttgcaa aactcaggag
    29341 gtaagcaatg aggacactac tttcggtgaa gtgggggagt taaagtcacc tgctatgcag
    29401 gtgactttat ccaggcgaac gcgttatcag aacgcgtagt taaagttagt accgaacagc
    29461 caggctttac cttcagactc gaactggtat gggccttcgt taattttcac gctctgaccg
    29521 tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta agtcgtacct
    29581 gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg agccggaact
    29641 gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta gtaataagtg
    29701 gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa cagcgtgtcg
    29761 ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta agccaagcta
    29821 tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca catttcaggc
    29881 aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg aattggcaaa
    29941 ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa gtcaattttc
    30001 acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata caggattccg
    30061 gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt attactgtcg
    30121 ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc aggagattgc
    30181 ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat tttcgcgcga
    30241 gcgtagacgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg atacgcaccg
    30301 cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc gcctgcataa
    30361 gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc ccaaccaaat
    30421 tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg cgcgatgtta
    30481 tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc cgggtcaata
    30541 taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc ggggttacgg
    30601 ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg gcccaggcca
    30661 gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga gataagtgcc
    30721 actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac cataacctca
    30781 atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa gtgtagggtc
    30841 tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca gctggaatgt
    30901 tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc agctggcaaa
    30961 atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat ttttaagtgt
    31021 gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact ttccggaagc
    31081 gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga aatcaacgat
    31141 gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg gcaccattat
    31201 ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaagccgcg cagaagcaga
    31261 acaaacgctg gcggcgctga ctgaaaaagc ccgtagcgtg gaatccgaac cgtgtaaaat
    31321 caccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt tcacttttgc
    31381 ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct cgggtacgta
    31441 cgcccctgtc agttgctggc aggggcgttt tcttttcttt ctcctgtctt cactgtgtca
    31501 tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatggtt aagactatga
    31561 tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca ggttttaccg
    31621 ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac gccttttgcc
    31681 cgccaggcga cggcttttca tggcattccc gcggttgatt tagggaagat ggtggtaggc
    31741 gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt ctttggtcag
    31801 gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg taccggaatg
    31861 aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt ccaggcggtt
    31921 gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc cggtggggca
    31981 gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgcgcgt gctggttgat
    32041 gtcaacaaag ctcgaaccat gagccagcga ctgaaactct tctctcgcct gcgtttgcgc
    32101 gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg gatgggcgac
    32161 accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga tgcattagcg
    32221 caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa agaagaggtg
    32281 atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa tattcgcggt
    32341 aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa acacggaacg
    32401 gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat cctgatgact
    32461 gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag ctacgcattt
    32521 actgcgattg atgtctggca ggacatgttg ctcggtccgg cctggtcaac accgctggcg
    32581 ctggaacgtg ccggtttgac aatgagcgat ctgacattga tcgatatgca cgaagccttt
    32641 gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc tcgtgaagta
    32701 ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt gcttggcggt
    32761 tcgattgctt acggacatcc cttcgcggcg accggcgcac ggatgattac ccagacattg
    32821 catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc tgccggtggg
    32881 cttggcgcgg caatggttct ggaggcggaa taatggaaat gacatcagcg tttaccctta
    32941 atgttcgtct ggacaacatt gccgttatca ccatcgacgt accgggtgag aaaatgaata
    33001 ccctgaaggc ggagtttgcc tcgcaggtgc gcgccattat taagcaactc cgtgaaaaca
    33061 aagagttgcg aggcgtggtg tttgtctccg ctaaaccgga caacttcatt gctggcgcag
    33121 acatcaacat gatcggcaac tgcaaaacgg cgcaagaagc ggaagcactg gcgcggcagg
    33181 gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg gctattcatg
    33241 gcgcttgcct gggtggtggg ctggagttgg cgctggcgtg ccacggtcgc gtttgtactg
    33301 acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta cccggttcag
    33361 gcggcaccca gcgtttaccg cgtctgatag gcgtcagcac agcattagag atgatcctca
    33421 ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat gacgttgttc
    33481 cgcactccat tttgctggaa gtcgctgttg agctggcgaa gaaggatcgc ccatcttccc
    33541 gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg ctgctgttca
    33601 aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg acagaacgca
    33661 tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggttat gacgccgaag
    33721 ctcgggcgtt tggcgaactg gcgatgacgc cacaatcgca agctttgcgt agtatctttt
    33781 ttgccagtac ggaagtgaag aaagatcccg gtagtgatgc accgcctgcg ccattaaaca
    33841 gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc actgcttgta
    33901 aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat catgcgctga
    33961 agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa gccagcgaac
    34021 gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt gcccatcgcg
    34081 atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg gtggcggaag
    34141 tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa tacgtcatct ttaccgattg
    34201 gtgatatcgc agctcacgcc acgcgacctg agcaagttat tggcctgcat ttcttcagtc
    34261 cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg gcgcaaacca
    34321 tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc gtgcgtgaca
    34381 aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct atccgcatgc
    34441 tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt ggttttccgg
    34501 taggtccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa attattcctg
    34561 tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt tcttcaattt
    34621 tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat ggtcagaaag
    34681 ggcgtaaaag caaaaaacag gtcgatcccg ccatttaccc gctcattggc gcacaagggc
    34741 aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg ctgaatgaag
    34801 cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat attggtgcgg
    34861 tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc gattctctcg
    34921 gcgcgggcga agtggttgca ataatgcaac gacttgccac gcagtatggt tcccgtttta
    34981 ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg aaaacaactg
    35041 caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg gcggagtgtt
    35101 ggtcaaaata taaacgcata ttgactatac ttacgccatt gaggtaaaaa acagcgtttc
    35161 attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg tttcagatga
    35221 aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt tatcatgcgt
    35281 cacggcgacg cagccctcga tgccgccagt gattccgttc gtcctctgac cactaatggt
    35341 tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga aatcgaacgc
    35401 gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg ggattgtctg
    35461 aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga tgttggtctg
    35521 gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact ggtgatctct
    35581 cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac gccgcctatg
    35641 tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg cacattcaac
    35701 tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgagt tccaaattat
    35761 gcccaatgag gtagattcat tgggcataat tgctcatcag gcagcggata tctgtcaacc
    35821 tcactactat cgaagactct ccccatctat aaataaaaca ctttctactt tcattcggaa
    35881 attattcttc tgcgcgtatc ttgattttgc ccaaagttac atgttgaaat gaataatgtc
    35941 tgtttgacac tcattgtcat ttattaatac agataataga gaaaatagag ttgaataatc
    36001 aggcaggatg taattgaaac ttgattaaat tctggcta
//
LOCUS       DAZFKM010000035        34576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFKM010000035 DAZFKM010000000
VERSION     DAZFKM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..1257)
                     /gene="amiC"
                     /locus_tag="ACHKAY_001873"
     CDS             complement(4..1257)
                     /gene="amiC"
                     /locus_tag="ACHKAY_001873"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO5700555.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1489..2820
                     /gene="argA"
                     /locus_tag="ACHKAY_001874"
     CDS             1489..2820
                     /gene="argA"
                     /locus_tag="ACHKAY_001874"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO5700556.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2882..4708)
                     /gene="recD"
                     /locus_tag="ACHKAY_001875"
     CDS             complement(2882..4708)
                     /gene="recD"
                     /locus_tag="ACHKAY_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO5700557.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4708..8250)
                     /gene="recB"
                     /locus_tag="ACHKAY_001876"
     CDS             complement(4708..8250)
                     /gene="recB"
                     /locus_tag="ACHKAY_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO5700558.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8243..11131)
                     /gene="ptrA"
                     /locus_tag="ACHKAY_001877"
     CDS             complement(8243..11131)
                     /gene="ptrA"
                     /locus_tag="ACHKAY_001877"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO5700559.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHLAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11307..14675)
                     /gene="recC"
                     /locus_tag="ACHKAY_001878"
     CDS             complement(11307..14675)
                     /gene="recC"
                     /locus_tag="ACHKAY_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO5700560.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14688..15011)
                     /locus_tag="ACHKAY_001879"
     CDS             complement(14688..15011)
                     /locus_tag="ACHKAY_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO5700561.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(14996..15403)
                     /locus_tag="ACHKAY_001880"
     CDS             complement(14996..15403)
                     /locus_tag="ACHKAY_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO5700562.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15400..15963)
                     /locus_tag="ACHKAY_001881"
     CDS             complement(15400..15963)
                     /locus_tag="ACHKAY_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5700563.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(15954..16424)
                     /locus_tag="ACHKAY_001882"
     CDS             complement(15954..16424)
                     /locus_tag="ACHKAY_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO5700564.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16608..17402)
                     /gene="thyA"
                     /locus_tag="ACHKAY_001883"
     CDS             complement(16608..17402)
                     /gene="thyA"
                     /locus_tag="ACHKAY_001883"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO5700565.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17409..18284)
                     /gene="lgt"
                     /locus_tag="ACHKAY_001884"
     CDS             complement(17409..18284)
                     /gene="lgt"
                     /locus_tag="ACHKAY_001884"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO5700566.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18435..20681)
                     /gene="ptsP"
                     /locus_tag="ACHKAY_001885"
     CDS             complement(18435..20681)
                     /gene="ptsP"
                     /locus_tag="ACHKAY_001885"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO5700567.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20694..21224)
                     /gene="rppH"
                     /locus_tag="ACHKAY_001886"
     CDS             complement(20694..21224)
                     /gene="rppH"
                     /locus_tag="ACHKAY_001886"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO5700568.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21577..21723)
                     /gene="ygdT"
                     /locus_tag="ACHKAY_001887"
     CDS             complement(21577..21723)
                     /gene="ygdT"
                     /locus_tag="ACHKAY_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO5700569.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21909..22598
                     /gene="mutH"
                     /locus_tag="ACHKAY_001888"
     CDS             21909..22598
                     /gene="mutH"
                     /locus_tag="ACHKAY_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO5700570.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22667..23380
                     /locus_tag="ACHKAY_001889"
     CDS             22667..23380
                     /locus_tag="ACHKAY_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5700571.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23518..23736
                     /gene="ygdR"
                     /locus_tag="ACHKAY_001890"
     CDS             23518..23736
                     /gene="ygdR"
                     /locus_tag="ACHKAY_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO5700572.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23844..24884
                     /locus_tag="ACHKAY_001891"
     CDS             23844..24884
                     /locus_tag="ACHKAY_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO5700573.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLAPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24916..26109)
                     /gene="lplT"
                     /locus_tag="ACHKAY_001892"
     CDS             complement(24916..26109)
                     /gene="lplT"
                     /locus_tag="ACHKAY_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO5700574.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26102..28261)
                     /gene="aas"
                     /locus_tag="ACHKAY_001893"
     CDS             complement(26102..28261)
                     /gene="aas"
                     /locus_tag="ACHKAY_001893"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO5700575.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28847..29878
                     /gene="galR"
                     /locus_tag="ACHKAY_001894"
     CDS             28847..29878
                     /gene="galR"
                     /locus_tag="ACHKAY_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO5700576.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29885..31147)
                     /gene="lysA"
                     /locus_tag="ACHKAY_001895"
     CDS             complement(29885..31147)
                     /gene="lysA"
                     /locus_tag="ACHKAY_001895"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO5700577.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31269..32204
                     /gene="lysR"
                     /locus_tag="ACHKAY_001896"
     CDS             31269..32204
                     /gene="lysR"
                     /locus_tag="ACHKAY_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO5700578.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32191..32883)
                     /gene="ygeA"
                     /locus_tag="ACHKAY_001897"
     CDS             complement(32191..32883)
                     /gene="ygeA"
                     /locus_tag="ACHKAY_001897"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO5700579.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33012..34430)
                     /gene="araE"
                     /locus_tag="ACHKAY_001898"
     CDS             complement(33012..34430)
                     /gene="araE"
                     /locus_tag="ACHKAY_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO5700580.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8446 a         9515 c         8567 g         8048 t
ORIGIN      
        1 acatcatccc cttctcgcca gcgtcgcccc atcggcaaaa tacgctttaa tccccgcaag
       61 aatagactcc gcaacttcct gctgaaaggt cgccgttttc agtttacgct cttcctcaac
      121 gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct ttagtacggc
      181 aaacccggcc tgttcaacct gatttttatg cagcttgttg attttaccga gcttattcag
      241 taccgcttta ccaaacttaa ggctgtcagc aatggtcagc gactgcacca tatcgaacat
      301 ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt ccgaggcgtt
      361 ctgggtttgt gccagatatt ttgccgcagt actggttgca cctttggttg agagcgcaaa
      421 cacagaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag agacaaacag
      481 gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga agatgtcttc
      541 attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc gcaaacggcg
      601 agctatttgc aataccacat ctttttctcg cgttttgtat ttccccaccg caccggagtc
      661 ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc ctgctttacc
      721 cggttgtgga ccactttgtg ctggcggcac ctgcttttcg aggtcgcctt tgttgtaatc
      781 ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat agaggtccat
      841 caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg gttttacgtt
      901 ttgctttaat tcaaaaacca tccgtacggt ctgcgggtca aattgcccga cgcgcgccga
      961 cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta ccgagttcag
     1021 gtttacatct tcgatatcca ccaccacgcg ttccgggtta ctcaacgcga actgcttata
     1081 tttcagctga cgatttgatt ctaccgtcac acgggtgtag ctggacgcag gccagacgcg
     1141 caccgccacg acctggctga ccgcagccag actgacctga cttacgctca atagccacat
     1201 ggcacccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc ctgacatgcc
     1261 tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt gaccggaaac
     1321 tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt ttatattcac
     1381 ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc tgcgaaaaaa
     1441 cagaataaaa atacactaat ttcgaataat catgcaaagg ggtgtgccgt ggtaaaggaa
     1501 cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa tacccaccgg
     1561 ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa tttctccagt
     1621 atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt ggtctatggc
     1681 gcacgtccgc agatcgacgc aaatctggcc gcgcatcacc acgaaccgct gtatcacaag
     1741 aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc gggaacattg
     1801 caactggaca tcactgctcg cctgtcgatg agtctcaata acacgccgct acagggcgcg
     1861 catatcaacg tcgtcagtgg caattttatt attgcccagc cgctgggcgt cgatgacggc
     1921 gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat ccatcgtcaa
     1981 ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac tggcgagagc
     2041 tttaatctga cctcggaaga gattgccact caactggcca tcaaactgaa agctgaaaag
     2101 atgattggtt tttgctcttc ccaaggcgtc actaatgacg acggtgatat tgtctccgaa
     2161 cttttcccta acgaagcgca agcacgggta gaagcccagg aagagaaagg cgattacaac
     2221 tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg cgtgcgtcgc
     2281 tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt ctcacgcgac
     2341 ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc aacaatcaac
     2401 gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat tctggtgcgc
     2461 cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca gcgcgataac
     2521 acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg ggaaatggcc
     2581 tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct gctggaacgc
     2641 attgccgctc aggcgaagca gagcggctta agcaaattat ttgtgctgac cacgcgcagt
     2701 attcactggt tccaggaacg tggatttacc ccagtggata ttgatttact gcccgagagc
     2761 aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga tttggggtaa
     2821 gcgatgaaaa tcgccggatg cgacatgcgt aacactcgtg cgtcgcatca ggcaattacg
     2881 tttattcccg cgaactaaac aatgccgcca gaccactgcg ccgctcagtg cgcgtggcga
     2941 ttgccgcact taatatgcgc tcatcggcat acagcgacag acggcgacgc gcgcgggtca
     3001 ctgcggtgta aaccagctct cgcgttacta ccggcgtgcg ttggctcggt aaaatcaacg
     3061 ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtcgtttcgt
     3121 gctctggcag gcggctcggt tgcacagact taatattgcc gtccggcatc gcaaaccaaa
     3181 cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac aacccaagcg
     3241 cgctgtcatt acgggcaatc atcaccggtc gaccttcgta ccaacgagag tgcggatgac
     3301 gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt ccagccacgc
     3361 caaacggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc tgaatgatta
     3421 aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc gcaagagctt
     3481 cctcaagcat cgcaatataa tcttctccgc tctgtaaaag tcgtttttcg atatcagtaa
     3541 aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc gccgcagcta
     3601 actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg cagagactgt
     3661 cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagtcccc gtcaggcggc
     3721 ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag atatcgccca
     3781 gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg
     3841 catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc attgacgctt
     3901 catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc
     3961 tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga atgcgtttct
     4021 tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg
     4081 cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg tcggccattt
     4141 gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct gggccgccgg
     4201 aaaccaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc cagttaattt
     4261 catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca tcaacctcaa
     4321 tggcatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac cacatgcgat
     4381 tcaaataaag acgatcgcca cagaggacca tcggcgtggg ttcatctccc ctgctgaccg
     4441 cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg
     4501 tcgccaacag cggatgcgac gcctcgttat tttccagtcg tgaaagcggc aaacagacgt
     4561 gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca ggatgttcat
     4621 ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg tgctccacag
     4681 cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat
     4741 ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc cttgttgcgg
     4801 atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt gacgatcata
     4861 gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat aaagctgata
     4921 ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg tgtaagccga
     4981 actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg
     5041 gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact ccagcggcgg
     5101 gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc tggcgatgag
     5161 cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt cggaaagctg
     5221 actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga tccactcggt
     5281 caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct gtacccagtt
     5341 cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccccgg
     5401 tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga cgctgaccac
     5461 gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt gaccacgctg
     5521 ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt gtagcgtcct
     5581 cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat caccagtttg
     5641 tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc gaagccctgc
     5701 cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt
     5761 gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca
     5821 aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac gttcgacctc
     5881 cgcaagctcg acgctttctg gcgcagcgtt aagatccaga acagcctcaa acgagtggcg
     5941 atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca accagaccag
     6001 cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat gtttatcact
     6061 ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa
     6121 ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgta gcagttcgct
     6181 gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca agttttctgc
     6241 aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccagat
     6301 ttgccgataa ccatcgaact cttcaactac cgcatcccac gcatgttcgt cattattcag
     6361 cgtttcgata tccagcgcgt tcagccccat cattgacgtt gccagcgcac tgcgcagggt
     6421 gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct gcgcttccag
     6481 agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcaacgttaa
     6541 ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacactga tgtccgaagc
     6601 acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg
     6661 tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat aatcgccaac
     6721 gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt cacctttaaa
     6781 cacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg gtatttcgcg
     6841 aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acactgttca ccattccagg
     6901 tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac gcgccttcat
     6961 ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt cgccaatcag
     7021 caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg
     7081 gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga tcgccgctgc
     7141 caacacctca ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa
     7201 acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc
     7261 gcgggtgatc accagatcgc ggatcgacaa tggttctgca agcagttgat cgatcgcctc
     7321 aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta agaaacgctg
     7381 ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt ctgcccaggc
     7441 gctgatcttt tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc
     7501 agaagattcg atcagcgcat caagttcacc cactgcgtcg cgccactgct gttttaccgt
     7561 atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat cgggcggcgg
     7621 tgctttgata accggtgctt cgccttgcag ataacgatta atatcgcgca gcaacgcctg
     7681 cggccctttc caggtttcaa agaccaccag ggcaatttca cgcggcagcg ggtagcagtg
     7741 gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt caatcagctg
     7801 ctgctcaaac agcataccgg attcaaaggc attcaggttg agcatgcgct ggcaaaaacc
     7861 gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca accactgcgc
     7921 ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat tgtcggtggt
     7981 ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac cacgcaattc
     8041 tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca gcgggcgggg
     8101 aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa
     8161 ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa
     8221 aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct
     8281 gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc
     8341 cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg accaccgcca
     8401 gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc acgattttat
     8461 cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt
     8521 cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa
     8581 actcttctgg cttcatcgcc cgcaattttg cctctgcggt tgggaaaaac gccttgtaac
     8641 gctcccacaa gaaggaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact
     8701 gacgccccac gctcattgga aatgcaaaca cggcatagcc caattgttct tcggtacgca
     8761 actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag gctgagctgg
     8821 tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac
     8881 cagctttttc aaagatgacg gattgttttt tatcgaccac gacatctttg ttacgacacc
     8941 actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt gttgcctggt
     9001 cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt aaggcgtcgc
     9061 gataggccag cacctctttc aacgtaatgg agggcaaaat tttgcgccgt tcatctcgcg
     9121 agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctcg aacgctttgc
     9181 ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca agctgatctt
     9241 ccgtggcggt atagctaaag tagccttcca gcaatgcctg gaacagctgc ggcagatgct
     9301 gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa aaacttatgc
     9361 caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga
     9421 gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc
     9481 tgacatcggc ttttggctcg ctggcaaaat aacggcttgg cgcatacacc acgcgcagat
     9541 tcgactcatc aacaatcagc tctggatggt catatttctt gtctgactta atcagcgaga
     9601 aatcgtcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg gcggcttttt
     9661 tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc gcatcgacaa
     9721 agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc gcattctgcg
     9781 gcgtcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg gcaatattga
     9841 ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc cattcgacgt
     9901 aatccatatc acgggtgatt gacggataac ggaagtcgat atccagcaca ttcgccagtt
     9961 catcgaagta ttgtttatcg atcccttttt cacgtaacaa attgaggtag ctaaaaatag
    10021 ccgccacaac ctgatcgcga ttcgccagac ctttatcggt taaagacgca gagatcgcta
    10081 atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc tcaactaatc
    10141 cctgcttttg cagccagtcg gaaagtgtac ctgggctgcg attaccaatc agataggtaa
    10201 tcaattcatc ggttttgcta cggaatttcg ccgagttgtt atcaatacga aactctacgc
    10261 gcaacacttt acgcggcagc gccgggacgt aatgaatgat aatgcccttt tgcgcgtcgg
    10321 tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg
    10381 tgtccgccgc catttttgcc aactccggca gcggtttatt gctgtaaata accgccttca
    10441 tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc accggattac
    10501 caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg
    10561 ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta cgcgccatgg
    10621 ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg agcaagggtt
    10681 ctgcaatagc atcagccagg cggtctaccg caccaggcaa ggcgtcgttc tcaacttcca
    10741 gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg tgcattttga
    10801 gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga ctcatatgtt
    10861 caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca acgggcacca
    10921 ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc accataccgt
    10981 tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct
    11041 gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca actaacaaca
    11101 ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa cttaattttc
    11161 tgtccagatt caacacgtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga
    11221 gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa agtaagcgga
    11281 tagattgcgc aatttttata cagcactcat gactgattaa agcgaaacag cggtaacagg
    11341 aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg ccgccagagc
    11401 ctttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga
    11461 aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc
    11521 gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg
    11581 gacatccctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct
    11641 gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta
    11701 ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg cgccacactt
    11761 aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa ccagccagtt
    11821 atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg gcgacaggca
    11881 atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa aatttcacca
    11941 aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca
    12001 tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct aagcccttcc
    12061 agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tacggaagtt cacctgcaaa
    12121 cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc
    12181 acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc
    12241 tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg ctggtagttt
    12301 tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac ccttgcctcg
    12361 ctttcatcac agttgagcgc ttcatcgtcc ggtagataat gactttgccc gatgtagtcg
    12421 atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg
    12481 atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa caggtagcgg
    12541 tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc
    12601 aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg
    12661 aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc
    12721 tggctgatac gttcttgatc cagacgctgc gccagttcat cacgcaatag tgacagcggc
    12781 accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg
    12841 atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg
    12901 cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg acgccagatg
    12961 tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat
    13021 tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccagc
    13081 aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag ttccagctcg
    13141 cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca ctggcgtaaa
    13201 taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagtaat
    13261 gccagcacgt cttccgacac aaagcggctg tctggcaacg ataacaggct gataaacgct
    13321 tccagtaccg gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg caggtaacga
    13381 tccgcaggtg cgctaccaaa cacagcctga ataaacggac tgtagctgtc gatatcagcc
    13441 accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cttccagcat cgccagcagg
    13501 cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg
    13561 ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca
    13621 ccagcaacgg cacggttttc cagttccaga atgtcagact gaatgttatg cagcaggtta
    13681 tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc agaaaggaga
    13741 taaatgtagt cgcgcccaag cttaccccat gaagccagca gcgggttgcc gacatcctgt
    13801 tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cacgaaatag cggcaattcg
    13861 cgatcttcaa cactgtgtcg acgctgacgg gtcagcagtt tcgccagata agcaggatct
    13921 ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt
    13981 ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat accgcatata
    14041 aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag
    14101 cgctgataga gattggcgcg gtgccaacgc ggttgtccga gttcatgggt atattccacc
    14161 agcgccttcc acaacggcgc ttgccaggcc tgtgcttctc ccagcccttc aaccaaatgt
    14221 cctgtttccc actgtgccag ccactccggg cgatagacca gatactggtc aaacaggtcc
    14281 gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagc cagataatgc
    14341 cgcaacaggg taaagtcttc gcgatccagc aattgcggca gcagagtcat cagtttccag
    14401 ctcatgctct gtttgttaaa ggcgctctct ttggggatct ccggtaacac ccggacgaac
    14461 atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat accaaacttt
    14521 tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag aatcatctct
    14581 ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc
    14641 acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc
    14701 gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg
    14761 atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc
    14821 gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg gtactggttt
    14881 ctgctggcga gactgttcat taatgttcgc tggatacccg ataacgcagt gacaatcatc
    14941 accatcagca ccatcgcaac cattacctcc ggcaggctaa agccttgttg attcttcagg
    15001 gaagctgaca taacgccctc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa
    15061 aaacaatgtt tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg
    15121 caaccaataa ggcttcatta tctgccagta aacgcaaaca aacccgggca tcggttccag
    15181 cgtactgcga gcactgaact gcgggctgcg tctgccagga gtgcattttc ccccacgcca
    15241 gcgccgactg aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg
    15301 caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca
    15361 tcaggaccag agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac
    15421 gctatagctg gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac
    15481 cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat
    15541 gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg
    15601 tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcagact cttttaccgg
    15661 ttcgcgatcc cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg
    15721 tccgacaatt tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag
    15781 atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca gcttacgggt
    15841 gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac
    15901 gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg
    15961 catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga aaccaccagc
    16021 caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt attgcgcagg
    16081 ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca
    16141 aagaccaatg gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac
    16201 cataacgtcc cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct
    16261 tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccatag ccgttgcgac
    16321 tgctgccagt attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc
    16381 gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc
    16441 gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta
    16501 cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta acggagggta
    16561 aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    16621 ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc gaaacggtag
    16681 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    16741 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtgtc gccccccgtc
    16801 cagacaaaat cgcccacttc cagatcacac tgctgcgcca tcatatgcac cagtaacgcg
    16861 tagctggcaa tgttaaacgg caggccgagg aaaacgtcac aggagcgctg ataaagctgg
    16921 caagagagtt tgccgtctgc cacatagaac tggaagaacg catggcacgg tgccagcgcc
    16981 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg
    17041 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc cggcgttggc
    17101 caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc ggcccattcg
    17161 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    17221 cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    17281 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    17341 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    17401 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    17461 acacccgcga caatcatcgg gatggaaaga atttgtccca tgctgatgta ctgcacccag
    17521 gcaccagtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    17581 taaccaatca ggaacaaacc tgagacagct cccattgggc gcggtttacg aatatacagg
    17641 ttgaggataa taaacagcac tacaccttcc agcaacagct cgtaaagctg tgatgggtgg
    17701 cgcggcagca caccgtaagt atcgaaaatg gattgccact gcgggttggt ttgcagcagc
    17761 aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt cgggtcaacg
    17821 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    17881 atgagtggtg cgataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag
    17941 ataatcatca ccacgataac gccaatcagg ccgccgtgga aggacatgcc gccgtcccag
    18001 acacggaaca gatacagcgg atcggccata aactgcggga agttgtagaa cagaacataa
    18061 ccaatacgtc caccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    18121 tttttggtcc agccactgcc cggacgattc gcccgtcgtg ttgccagcca cattgcaaaa
    18181 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    18241 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    18301 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    18361 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    18421 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccacg acgctccata
    18481 aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact acgctgcgca
    18541 agattttctg cttcggcata atcaatgcgc cgcagcaggt attttacccg cgctacagaa
    18601 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    18661 tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc ttcccgggcg
    18721 atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt cgccacccgg
    18781 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    18841 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac ttccagcatg
    18901 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca
    18961 cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc ttgtgaccat cggcaacaga
    19021 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    19081 atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg
    19141 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    19201 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    19261 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    19321 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    19381 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    19441 tcaatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc cggatcgacc
    19501 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    19561 atatccgcgc ccatcacggt agggatcccc agcgcacgta ccatgatcgc agcatgggag
    19621 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    19681 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    19741 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc
    19801 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    19861 gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    19921 agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    19981 gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    20041 ccggtcagtc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    20101 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    20161 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaggg cagtcaactg cgactgggaa
    20221 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    20281 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    20341 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    20401 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    20461 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    20521 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    20581 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    20641 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    20701 cttttacgtc gataagcaga tgcgttttgt ggttttggcg tattttcctg cagtgacatc
    20761 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    20821 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    20881 gtttgcatat tgatttctgc atcgccgctt accagctgca agagaaacca tttttgtttt
    20941 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    21001 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    21061 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga
    21121 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    21181 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat
    21241 aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca
    21301 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    21361 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    21421 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatagac attttaaaat
    21481 attcattatt agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac
    21541 ccagctcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    21601 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    21661 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    21721 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgttg
    21781 cgcaactcga ttaccggcag cctaaaaagc aatataaatc agagaattga acaacacata
    21841 tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt taaccaaggt
    21901 atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt
    21961 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    22021 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    22081 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    22141 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    22201 ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccatgtgcgc cacaagctca
    22261 aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg
    22321 taggatcacc gttgctgtgg agcccgaatg aagaggaaga ccgacagcta cgcgaagact
    22381 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg
    22441 gggaatattt acagataaga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    22501 gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca
    22561 ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    22621 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    22681 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    22741 tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca cagcacaacg
    22801 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    22861 atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat
    22921 ttctgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    22981 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    23041 cctcggcgct atcgtgcaga ttatgctgct ggatattatc tttagcctcg actcggtgat
    23101 tacggctgtg ggtctgtcag atcacctgtt tattatgatg gcagccgtgg tgattgccgt
    23161 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt
    23221 aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt
    23281 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    23341 aagcctcaac ttgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc
    23401 gggagggcgt actcactcct tttaccctca cagtttaaga ttttcctgct ttcaagatat
    23461 ataacgtcgg tttataaaca gactattatc ataggtaggc caaatgagga taaccggatg
    23521 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    23581 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    23641 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    23701 aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgg
    23761 ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc
    23821 gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag
    23881 tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc
    23941 acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt
    24001 acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc
    24061 tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc
    24121 gcaataatga caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag
    24181 cgctgcatga cagcctcaag cgcctacaga ctgattacct cgatctttat caggtgcact
    24241 ggccgcagcg cccgaccaac tgcttcggca aactcggtta tagctggacg gattctgcgc
    24301 ctgcggtttc gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa
    24361 ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac ctgcatctgg
    24421 cggacaaaca cgatctgccg cgtattgtca ccattcagaa cccttacagt ctgttaaacc
    24481 gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct
    24541 attcgtgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca aaacccgctg
    24601 gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag
    24661 ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggcccctgct cagatggcgc
    24721 tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg
    24781 atcagctgaa aactaacatc gaaagtttgc atctggagtt aagcgaagac gtattagctg
    24841 aaattgaagc ggtgcatcag gtttatactt atccggcacc ataagattaa cgccggttaa
    24901 aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta ttgccagcgc
    24961 aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctatca ttaccgccag
    25021 cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt gtactgcaat
    25081 cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac
    25141 gacaaaaaag ccccccatca cgccaatcag catcaacaag gcataggctg gcagcagctc
    25201 gtgttgcagg gaaaaaatca gtaccaccac gccaatcaaa atcccggctg gcatacagcg
    25261 tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcgccaacca cgataccaat
    25321 cgctaccatc gcgttgagat aggtgggcgt agcgttatcg gtaatgccca gcgccaccgg
    25381 tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc
    25441 caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat tcaggaaact
    25501 gcgggtcatg ttgatgagat tccaggactg ccccggacgc gccgccgcca gtttgggaat
    25561 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gggcgacgag
    25621 gacatgccag tcagccagca caccaccggc tacggaaccg agcaaaatcg ccgctatggt
    25681 agaagcttcc attaaaccgt tagctttcac taacttacta cccgtggtta attcgccgag
    25741 aataccgtat ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag
    25801 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    25861 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgcaaaaag
    25921 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    25981 cgctttcagt aacgccagag tggcaaacag tagggcatta tcgccaaacg cagagagaaa
    26041 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    26101 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    26161 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    26221 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg
    26281 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    26341 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    26401 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    26461 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    26521 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    26581 gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac aggcgcgcat
    26641 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    26701 aaacgacagg cgcgcactcg gttacaccat agccttcaag gatgcgcagg ccaaatttat
    26761 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    26821 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    26881 cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg taatgcagcg
    26941 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    27001 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    27061 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    27121 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    27181 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    27241 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    27301 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    27361 ccgccgtaat agcactggtc agccctttta ccccagcagt gtagttcatc attgcgggaa
    27421 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    27481 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    27541 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    27601 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    27661 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    27721 ggtcacgggc acgcggcgca tccggcatcg ccacctgcgt tggtggcaaa atatgcagag
    27781 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    27841 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    27901 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    27961 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    28021 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    28081 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    28141 caataaaaga gacgtgatta ggcgtaatta gaacgcgctc gcccttcagt gcctgggtgt
    28201 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    28261 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    28321 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    28381 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct
    28441 tgattgtggt ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa
    28501 tgcaacgagg tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac
    28561 ctgcgcatct gcgcaggctg gtgtaattca tgtgctcaac ccgaagttga cttcacctat
    28621 caatacctct gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg
    28681 caacagcgtt tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt
    28741 tgcgtttggc tcgcattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt
    28801 ccgtaacact gaaagaatgt aagcgtttac ctactaaggt attttcatgg cgaccataaa
    28861 ggatgtagcc cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc
    28921 acctaaagcc agtgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta
    28981 tcacccgaac gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat
    29041 cgttggtgat gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc
    29101 ttatcacacc ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg
    29161 tcaggctatt gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat
    29221 gatcccggat gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa
    29281 ccgtatcctg cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg
    29341 gctggcaacg cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa
    29401 ccactctatt tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag
    29461 tggtattgcg gccaatgacc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga
    29521 acaggcaatg accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa
    29581 cgattcaatg gcggcgggtg cgatgggcgt tctcaatgat aatggtattg atgtaccggg
    29641 tgagatttcg ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct
    29701 gaccaccgtg cgttacccaa tcgtgacgat ggcgacccag gctgccgaac tggctttggc
    29761 gctggcggat aatcgccctc tcccggaaat cactaatgtc tttagtccga cgctggtacg
    29821 tcgtcattca gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc
    29881 gcagttaaag caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc
    29941 gcgcctgacc attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg
    30001 acattgatgc gccatatgcc cctgtatcat gcagtaccag ataatcaccc gccttcactt
    30061 ccggcaaggc gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt
    30121 cacataatgg cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat
    30181 cagctgccag ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt
    30241 tgaacccggc atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct
    30301 gagtaattaa cacgccagac tgcgctacca gaaagcgacc cggttcaatt tccagtttca
    30361 cagggtggcc caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat
    30421 gttcggtatc aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag
    30481 aaatagcctg taaatcctga ccgaattcga tgacctgacg taccatagca ccacacacct
    30541 gttccagatg ggcataatca acgccagaac caatgtgcat gtgaatgccg accagctgca
    30601 aatgatgacg ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct
    30661 tgctgttttc gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccgggttaa
    30721 cgcgcagcca tacccgatgc cctggcgaaa cctggcccag ttggtcgagc atatcaacag
    30781 aacccgcatt caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa
    30841 catctgccgt aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg
    30901 cacgctctat ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaatc
    30961 gcaaaatatg aatattggaa caggctttct gtgcaaagcg caccacatca aactgtttca
    31021 gcgctgcaat ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt
    31081 cagcgggcaa acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat
    31141 gtggcataac aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat
    31201 aaaaaatatc gatttatgtc gagtctatgc aaaaatgata tggattaccg gattgcgaga
    31261 gagcgctaat ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg
    31321 ccggaagcct gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg
    31381 aactggcgcg ctttgagaag gtgatcgggc taaaattgtt tgagcgcgta cgtggacgat
    31441 tacatcctac cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac
    31501 tggatcgcat tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta
    31561 ttgcctgcct gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg
    31621 cacgttatcc cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt
    31681 ggctctcggc ccagcgtcat gatttaggac tcactgagac actccatacg cctgcgggaa
    31741 cagaacgtac cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc
    31801 tggcggtaaa aaaggtatta acgccggatg attttcaggg tgagaactac atcagccttt
    31861 cccgtactga cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac
    31921 gacgcatgat cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg
    31981 taggtgtttc ggtggttaac ccgctcaccg cactggatta tgcggcaagc ggtttagtgg
    32041 tacggcggtt cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc
    32101 cgtcatcagc gctggttcag gcgtttagtg agcatttaca agcggggtta ccgaaactgg
    32161 tcacttctct tgacgcgatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc
    32221 ggcatggatc gccgcagtat caaacacagg caaaacactg cgctcttctg gaaccaataa
    32281 accaatttct gtgcagccaa aaatgacacc ctgcgccccc tgttctgcaa ggcgagcaat
    32341 cacttgcgca taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa
    32401 aataatctga ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa
    32461 ttgttccgtc agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag
    32521 cgccacacga gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa
    32581 gaaaggcaga gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca
    32641 taacacaata ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc
    32701 cccggtttta tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct
    32761 atgtagcagc acttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat
    32821 cagacgatag taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt
    32881 cattcctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc
    32941 ctcaacggcc aggtgcccga tctctattaa cgaaaaaagg gccggatgta cagcacatcc
    33001 ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg
    33061 atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat gcccacaaac
    33121 gcaatgttca gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt
    33181 gtcaggaagg tcgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta
    33241 ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc
    33301 gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac
    33361 aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg
    33421 cctaacgcca tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc
    33481 gtaaagaccg caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc
    33541 atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc atagtacatg
    33601 atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca
    33661 cgacggacgt tacggttgat cttaaacagt gcccaaccgc cttgttttaa cttcaggctt
    33721 tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac
    33781 acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg
    33841 aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa cattgcgcgc
    33901 cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg
    33961 accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa
    34021 agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg
    34081 gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg
    34141 atggcccccg ccatcaggct gtatttacga cccaggcgga acgacagcca accattaaac
    34201 agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg
    34261 gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga
    34321 ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta
    34381 tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc
    34441 cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacgcaggt gtcaggtcgg
    34501 aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg atattccaat
    34561 tttcaaatta agttga
//
LOCUS       DAZFKM010000036        34134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFKM010000036 DAZFKM010000000
VERSION     DAZFKM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            27..767
                     /gene="yadV"
                     /locus_tag="ACHKAY_001899"
     CDS             27..767
                     /gene="yadV"
                     /locus_tag="ACHKAY_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5700581.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            802..3402
                     /gene="htrE"
                     /locus_tag="ACHKAY_001900"
     CDS             802..3402
                     /gene="htrE"
                     /locus_tag="ACHKAY_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO5700582.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3419..3988
                     /gene="yadM"
                     /locus_tag="ACHKAY_001901"
     CDS             3419..3988
                     /gene="yadM"
                     /locus_tag="ACHKAY_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5700583.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4003..4605
                     /gene="yadL"
                     /locus_tag="ACHKAY_001902"
     CDS             4003..4605
                     /gene="yadL"
                     /locus_tag="ACHKAY_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO5700584.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4632..5228
                     /locus_tag="ACHKAY_001903"
     CDS             4632..5228
                     /locus_tag="ACHKAY_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO5700585.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5212..6555
                     /locus_tag="ACHKAY_001904"
                     /pseudo
     CDS             5212..6555
                     /locus_tag="ACHKAY_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            6668..7462
                     /gene="panB"
                     /locus_tag="ACHKAY_001905"
     CDS             6668..7462
                     /gene="panB"
                     /locus_tag="ACHKAY_001905"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO5700586.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7474..8325
                     /gene="panC"
                     /locus_tag="ACHKAY_001906"
     CDS             7474..8325
                     /gene="panC"
                     /locus_tag="ACHKAY_001906"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO5700587.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8399..9301)
                     /gene="rpnC"
                     /locus_tag="ACHKAY_001907"
     CDS             complement(8399..9301)
                     /gene="rpnC"
                     /locus_tag="ACHKAY_001907"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO5700588.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            9575..9955
                     /gene="panD"
                     /locus_tag="ACHKAY_001908"
     CDS             9575..9955
                     /gene="panD"
                     /locus_tag="ACHKAY_001908"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO5700589.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(9959..11188)
                     /gene="yadE"
                     /locus_tag="ACHKAY_001909"
     CDS             complement(9959..11188)
                     /gene="yadE"
                     /locus_tag="ACHKAY_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5700590.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11252..11692)
                     /gene="yadI"
                     /locus_tag="ACHKAY_001910"
     CDS             complement(11252..11692)
                     /gene="yadI"
                     /locus_tag="ACHKAY_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5700591.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11797..12567)
                     /gene="yadH"
                     /locus_tag="ACHKAY_001911"
     CDS             complement(11797..12567)
                     /gene="yadH"
                     /locus_tag="ACHKAY_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5700592.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12564..13490)
                     /gene="yadG"
                     /locus_tag="ACHKAY_001912"
     CDS             complement(12564..13490)
                     /gene="yadG"
                     /locus_tag="ACHKAY_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5700593.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13599..14261
                     /gene="can"
                     /locus_tag="ACHKAY_001913"
     CDS             13599..14261
                     /gene="can"
                     /locus_tag="ACHKAY_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO5700594.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14302..14838)
                     /gene="hpt"
                     /locus_tag="ACHKAY_001914"
     CDS             complement(14302..14838)
                     /gene="hpt"
                     /locus_tag="ACHKAY_001914"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO5700595.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15044..17434
                     /gene="gcd"
                     /locus_tag="ACHKAY_001915"
     CDS             15044..17434
                     /gene="gcd"
                     /locus_tag="ACHKAY_001915"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO5700596.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17481..19031)
                     /gene="cueO"
                     /locus_tag="ACHKAY_001916"
     CDS             complement(17481..19031)
                     /gene="cueO"
                     /locus_tag="ACHKAY_001916"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO5700597.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19197..19544
                     /gene="yacC"
                     /locus_tag="ACHKAY_001917"
     CDS             19197..19544
                     /gene="yacC"
                     /locus_tag="ACHKAY_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO5700598.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19650..20516
                     /gene="speE"
                     /locus_tag="ACHKAY_001918"
     CDS             19650..20516
                     /gene="speE"
                     /locus_tag="ACHKAY_001918"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO5700599.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            20532..21326
                     /gene="speD"
                     /locus_tag="ACHKAY_001919"
     CDS             20532..21326
                     /gene="speD"
                     /locus_tag="ACHKAY_001919"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO5700600.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21364..21726)
                     /gene="yacL"
                     /locus_tag="ACHKAY_001920"
     CDS             complement(21364..21726)
                     /gene="yacL"
                     /locus_tag="ACHKAY_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO5700601.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(21901..24498)
                     /gene="acnB"
                     /locus_tag="ACHKAY_001921"
     CDS             complement(21901..24498)
                     /gene="acnB"
                     /locus_tag="ACHKAY_001921"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO5700602.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            24853..26610
                     /gene="yacH"
                     /locus_tag="ACHKAY_001922"
     CDS             24853..26610
                     /gene="yacH"
                     /locus_tag="ACHKAY_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO5700603.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(26681..28105)
                     /gene="lpdA"
                     /locus_tag="ACHKAY_001923"
     CDS             complement(26681..28105)
                     /gene="lpdA"
                     /locus_tag="ACHKAY_001923"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO5700604.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28430..30322)
                     /gene="aceF"
                     /locus_tag="ACHKAY_001924"
     CDS             complement(28430..30322)
                     /gene="aceF"
                     /locus_tag="ACHKAY_001924"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO5700605.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30337..33000)
                     /gene="aceE"
                     /locus_tag="ACHKAY_001925"
     CDS             complement(30337..33000)
                     /gene="aceE"
                     /locus_tag="ACHKAY_001925"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO5700606.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33161..33925)
                     /gene="pdhR"
                     /locus_tag="ACHKAY_001926"
     CDS             complement(33161..33925)
                     /gene="pdhR"
                     /locus_tag="ACHKAY_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO5700607.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8541 a         8887 c         8231 g         8475 t
ORIGIN      
        1 ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca acagctttat
       61 gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc atttcgggta
      121 ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga
      181 ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct gagcctggca
      241 gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc
      301 aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga gagagcgtgt
      361 tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa
      421 gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg gatggattga
      481 agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta
      541 aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc agtggtgatt
      601 tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca tttagtgatg
      661 aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta catttttacg
      721 ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca ggcaaggata
      781 taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca taatcatctg
      841 acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg tgctgaactt
      901 gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga tctcagccgg
      961 tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta tgtcaacgat
     1021 caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa aaacgcacaa
     1081 gcctgtatca cacttaagaa tttattgcag tttcatataa attctcccga tataaataac
     1141 gaaaaagccg ttctgcttgc cagggatgaa acactcggca attgccttaa tttgacggaa
     1201 attatccctc aggcttctgt tcgttatgac gttaacgagc aacgtctgga tatagacgtt
     1261 cctcaggcat gggtaatgaa aaattaccaa aactatgttg atccatcgtt atgggaaaat
     1321 ggcattaatg cggccatgtt gtcatacaac ctcaacggat atcatagtga aactcctggt
     1381 cgaagaaatg acagcattta tgctgctttt aacggtggga tgaatttagg tgcatggcga
     1441 ctgcgtgcct caggcaacta caactggatg accgattctg gcagtaatta tgattttaag
     1501 aatcggtata tacagcgtga tatcgcctcg ctgcgttctc aactcattct tggtgagtct
     1561 tatacaacgg gcgaaacctt tgattctgtc agtatccgcg gcattcgttt atacagcgac
     1621 agccgcatgt tgcctccgac attagcgagt tttgcgccta tcattcatgg cgttgctaat
     1681 accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac gactgtgccg
     1741 ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt acggcagcga tcttattgtt
     1801 actgtcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc atcggtcgtt
     1861 caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt cttaaaagat
     1921 gatattcagg atgagcctaa tttatttcag gccagctact actacggtct gaataactat
     1981 ctaacgggtt atactggtat tcagattacc gataataact ataccgctgg tttgttaggt
     2041 cttggtctga atacttcagt cggtgcattt tctttcgatg tgactcactc caatgttcgt
     2101 ataccggatg ataaaacata tcagggacaa agttatcgtg tttcctggaa caagttattc
     2161 gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca gaattacctt
     2221 ggtcttaatg atgcactaac tctaattgat gaagtgaaac atcccgaaca agatcttgaa
     2281 ccgaaatcca tgcgtaatta ctcgcgcatg aaaaatcagg ttacggtcag tattaaccaa
     2341 ccgctaaaat ttgagaaaaa agattacggt tcattttatc tttccggaag ttggtccgat
     2401 tactgggctt ccggacaaaa tcgtagcaat tactctattg gctacagtaa cagtgcatcc
     2461 tggggcagct acagtgtcag tgcccagcgt tcatggaatg aagacggcga cactgacgat
     2521 agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga acaacggact
     2581 tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa taaccaactc
     2641 aacgtcagta gcagtggcta tagtgataac gctcgcgtca gttatagcgt aaatactggc
     2701 tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc cagctatgaa
     2761 tcaccgtggg gaaccctggc aggttcagtt tctgcaaata gcgataacag tcgtcaagtg
     2821 tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt cagtaatgac
     2881 agttttagcg actccgatac actggcggta gttcaggctc caggtgctca aggagcgcga
     2941 ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag cgctctttct
     3001 ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga tgttgaatta
     3061 aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc tgattttgaa
     3121 accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa aaatattcca
     3181 tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg acagggtgga
     3241 caagcatttg ttcgtggtat tgagcagcag ggaaatatca gcattaaatg gctcgaagaa
     3301 agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga aaaaatagcg
     3361 caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg gacgtcaaat
     3421 gataaaaata acgccacata aaatagcgat actgatggga atattattat caccatcagt
     3481 atttgcaacg gatattaatg tagagtttac agccactgtc aaagcgacaa cctgtaacat
     3541 cacacttact ggtaataacg tcacgaatga tggcaataat aactacacat tgagaatccc
     3601 taagatgggt ctggataaga tcgcgaataa aacgacagaa tctcaggctg attttaaact
     3661 ggttgctagt gggtgcagca gtggcatcag ttggattgat accactctga gcggaaatgc
     3721 atcatcaagc tcacctaagc ttattatacc gcagtctggt gattcatctt cgacgacaag
     3781 taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc ttaaacctaa
     3841 cagtgcggaa aagatacgct ggagcacaga cgagatgcag cccgataagg gtcttgaaat
     3901 gaccgttgcg ctacgtgaaa cagatgcagg gcaaggcgta ccggggaatt ttcgtgcact
     3961 ggcaacgttt aatttcatct atcaataaga aggataatta agatgacatt taaaaattta
     4021 cgttatggat tatccagcag tgtcgttttg gctgcctcat tgttcagcgt accctcttat
     4081 gcggcaacag acagcattgg gctgaccgtg attactaccg tagaaatagg tacttgtacc
     4141 gctacattag taaatgactc tgatcaggac atttctgttg ttgactttgg cgatgtatat
     4201 atttctgaaa tcaatgccaa gaccaaagtt aaaacattca aactcaaatt caaagactgt
     4261 gcgggtatcc ccaataaaaa agcacaaata aaattaacca agcgagccac atgcgaaggt
     4321 actgctaatg acggtgcggg gttcgcaaat ggatccacag ccgcagataa agcaagtgcc
     4381 gtcgccgttg aagtctggag caccgaaact ccggcaacag ggagcgcaac acaatttagt
     4441 tgtgtgacac cagcatctca agaggtcact atctccaatg cagccaacgc ggttgtttat
     4501 tatccgatga gtgctcgact ggtcgtcgaa aaaaataaaa ccgtaagcaa tgtcactgcg
     4561 ggtaaatttt ctgcacctgc cacatttaca gtaacctata actaacagac tgacagatcc
     4621 aggaactata tatgcatccc actcaacgta agctgatgaa gagaataatt ctgtttctgt
     4681 cactacagtt tcccattacc tatccagcca tcgctgggca ggatattgac cttgttgcca
     4741 atgtaaaaaa cagcacttgc aaaagcggaa tcagtaacca gggtaatatt gatcttggcg
     4801 tcgttggggt gggatatttt tcagataatg ttactcctga aagctatcaa ccaggtggaa
     4861 aagagttcac tgtcacagta tcagactgtg cattacaggg aactggcgat gtgcttaatc
     4921 agttacatat tgattttaga gcccttagcg gtgtcatggc tgcaggttca aggcaaatat
     4981 ttgccaatga agtcgcaacc ggggctaaaa atgtcggtgt cgttctcttt tccattcagg
     5041 acccgaccaa tatattcaat gttattagtt cagcaggaaa ttctcgttcc gtgtatccag
     5101 taatgacttc tgccctgcat aattcttcat ggaaatttta tgccagaatg caaaaaatcg
     5161 atcccgcgct ggatgtcata tctggccagg taatgagtca tatattagtt gatgtgtact
     5221 acgaataata gatcaaaaca aataactgcg actgttacag cagtagatgg tggagtgata
     5281 atgaagaaat tcttcagaca ttttcttttt ttaattctgt gcttgagttg ttataccgcg
     5341 agtgctggca ctgatgataa cgtaggatat attgttggga atagttacgg cgttggtccg
     5401 tcagatcaaa aatggagaga aacggggccg aatggcgatg ctactgtcat attcagatat
     5461 gcaacaagta caaataattt agttttctac aaaccgacgc agcttggacc tacaggtgta
     5521 aaattacagt ggagtcagtt agataccgct tctggtggtg gttttcttta ttgcaacaga
     5581 agtgatagca caagtggtag cgcaatgcgt attgaaaatg caatggttga ctcaggtaaa
     5641 atgtatggct cccataaatt atttaataca tcagttcctg gtttgtatta cacattatta
     5701 atttcaaaca tgtggtcagc ttacggtacc gtaactaacg ttagttcacc tgggatatac
     5761 attggtgact ctgcagaaca atatttttcg tggtataatc caagcgaaga cgtgttatat
     5821 tagagttgca ataatgcgaa tagcacccgt aaatactggg ctgtaggtgg tatttatcag
     5881 acccttacaa ttgaattcta tacagataca aactttgatc caacagtaac tcaacagatc
     5941 aaattatcca gctcatcaaa ttatctgtat tcatttaaag cctatggcgc aggccaaggt
     6001 ataaatgagc atagttattt tatcaaaatc gatttcgatc tgctcaatgt caagttgact
     6061 aatcccactt gttttaccgc tatgcttagt ggaacttctg tgactggttc tacggtaaaa
     6121 atgggtgaat atagtgcaga acaaatcaga aacggtgcca caccggttcc ttttgatatt
     6181 tcacttcaaa actgcgttcg tgtgactaat attgagacaa aattagtttc aacaaaggtt
     6241 ggtactgaaa acgggcaact ccttggtaat actctcacag gtaatgacgc agcaaaagga
     6301 gtcggtgtac tcatagaagg tttagcaact agtaaaaatc ctctaatgac attgaaacct
     6361 aatgattcaa attctgttta taaagattac gacccgagag gcaaagacga tacaacagga
     6421 ggagtttacc cggatcaaga tacaggtata acataccctc tccatttcca ggccacgcta
     6481 caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag tactttccag
     6541 gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcag taattgataa
     6601 atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc atttatcagg
     6661 atacgttatg aaaccaacca ccattgcttc acttcagaaa tgtaagcagg ataaaaagcg
     6721 tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg atgaaggact
     6781 taacgtcatg ctggtaggcg actcgctggg catgacggtt cagggtcacg actccaccct
     6841 gccggtgacc gttgctgata tcgcctacca cactgccgcc gtacgtcgcg gcgcgccaaa
     6901 ctgcctgctg ctggctgatc tgccgtttat ggcgtatgcc acgccggaac aagcctttga
     6961 aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg gcggtgagtg
     7021 gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg gtcacttagg
     7081 tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc gcggcgatga
     7141 agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg cacagctgct
     7201 ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgatgcac tggcgatccc
     7261 ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga tgcacgacgc
     7321 cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg ccgaaacggg
     7381 cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg tttatccggg
     7441 cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac cctgccgctg
     7501 ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct ggtgcctact
     7561 atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc ccgcgccgat
     7621 gtggtcgtcg tcagtatttt cgttaaccca atgcagtttg accgcccgga agatctggct
     7681 cgttatccac ggaccttgca agaggactgc gaaaagctaa acaaacgtaa agtggattta
     7741 gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca cacttacgtt
     7801 gacgttcctg gcctttcgac catgctggaa ggtgccagcc gtccgggaca ttttcgcggc
     7861 gtttcgacta ttgtcagcaa gctgttcaac ctggtccagc cggacatcgc ctgcttcggt
     7921 gaaaaggact ttcagcaact ggcgctaatc cgcaaaatgg ttgccgatat gggcttcgat
     7981 attgaaattg tcggcgtccc gattatacgc gccaaagatg gccttgcgct cagttcccgt
     8041 aatggttatc tgacggcgga acaacgcaaa attgcgcccg gtctgtacaa agttttaagt
     8101 tcaattgccg acaaattgca ggctggggaa cgggatctcg atgaaattat taccattgcg
     8161 gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg cgatgccgac
     8221 acattgctgg aagtttctga aaccagcaaa cgggcagtaa ttctggtagc cgcctggctt
     8281 ggcgatgctc gcctgatcga caacaaaatg gtcgagctgg cgtaatactt aactggcgct
     8341 acggctgatg gcgccagtta ttaatttacc ccacgtcatc tgtcataaca caggtaactt
     8401 aaataaggtt aattacctta tcaatctcag caagaggtaa atttgccatc tctgcaacgt
     8461 cttcccgaga cattccttta cttagaagac gcagggcgaa ttcttgcctt tcctcctgtc
     8521 ttccctgctg aatccccttc tcaatccctt tctcttcaaa ccactgcgcc agcgtcatca
     8581 tagactcccc tcccgtttcc ctgtctctca gcacaccata aaacaaatcc gcttgttcag
     8641 tatgaccgcg ttgcagcata tagttttgca tggcaactaa ctgacttccg ctagtgtacc
     8701 cttcgtcaat cagcgtgacc agttgctcca gcaataacat taagtcgcgc tggcgaatat
     8761 gtttttgcag tagttcgaga atcgcaatcc gccgatgttg catgatttcg tcatccggtg
     8821 tgatggtgat atccaccagc gggaaaggac tgttatagac gcgtcgtgcc agctccggcg
     8881 agtaaaatat atcaaaccag cacattgaga gcggataagg tgtggcctcg ccctgataaa
     8941 acagtatcgg caccaccagc ggcagcttat cgtgatccgc ctccagatgc cggtgcatgg
     9001 cagcaataga ataacgcatc atgcgaaagg ccattttctt atccggcttg ctttggtgtt
     9061 caatcacaac atgcagataa ccgggattgc cctgcatttg cacggaatag agcacgtccg
     9121 tgctgtgtcc tttcaggctc tcttcaatga aactccccga ctctaaatga agcgtgttga
     9181 ggtcacaaag ttctcgtaat tccactggca aatgtatctc cagaaagtcg cgagccgtct
     9241 ccgcgtgcat taaaaattgt ttaaacactg catcatgcgg tgtggtactc ggtgcatcca
     9301 tcttccctgc cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgtgccatcg
     9361 tcagccacct ctttagtgtt taccgagcag cgttcagaga aatttcaatt ggttacagcc
     9421 gttacatggc acgcttcggc acggtacgca aactgctgat gtaaatgccg tcagatgagt
     9481 agacactaaa caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcatcgcct
     9541 gcttcgttaa cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa
     9601 actccaccgc gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga
     9661 ccaggatttt cttgacgcag ccggtattct cgaaaacgaa gccattgata tctggaatgt
     9721 caccaacggc aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat
     9781 ttctgttaac ggtgcggcgg cccactgcgc cagtgtcggc gatattgtca tcatcgccag
     9841 cttcgttacc atgccagatg aagaagctcg cacctggcga cccaacgtcg cctattttga
     9901 aggcgacaat gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cttgattgtt
     9961 atccctgcgg ctggttactc accagccgcg acatcgtctc cagcgaatcc gttcttaaga
    10021 tataaagtcg ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg
    10081 tcaccgccag gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca ttaaatccgc
    10141 caaacggata cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac
    10201 gtgcaaaatc aaacagaata ttgtgctcac tacggctcag taatatgggt cggcgatagc
    10261 catccacccg atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg cgaatttcgt
    10321 taagctcaga aacgctcata aattgcagcg attttgggtt ccacttctgc gggtgacgtt
    10381 tgatgcgcga ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc aatacaggat
    10441 acgcatagcg gctcaccgac ttgaggccat catcaaaggt aatcaccacc gctcgcgcag
    10501 ggagattgat cttattcttc acgtaacctt ccagctgcgc catgctcagt gtcgcgtatc
    10561 ccctgtcgcg cagccaggcc atttggttat tgaaagcgcg taccgaggtg gtcgtcgaag
    10621 tatggcgaaa acgggtgttt tcttcatcgc gcagaatatg gtgataggtt agcaccggca
    10681 ggccattatc gggttgggca tccagcgcgc tgatataggc gagtcgatcg ccaatacgga
    10741 tctgatacca ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat
    10801 tgtccgccag taccccaaat ggcgcactgc ccgcactcgg cgcgttatag accggcgtat
    10861 ctttccaggt aattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt
    10921 caactttttg tcgcccctga actggctcga gatgcccttt atcgataaac cctttaccaa
    10981 agccaaaatt aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgcccgg
    11041 cccgaatatt ccccacggtc accatcttgt cgccaatttg cgcccagatc gcagcatttt
    11101 cgatggtttg catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca
    11161 gcaggagaat aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt
    11221 attgtagcaa aagcgctata agaatcgtgg gttaatactc atacaaatta tgtttgagga
    11281 cgaaaggcgg atttttttgt tgttggtgcc aaagactact cacctccggc ccacccagtt
    11341 cgacaatacg ctcgcgaaac tctgaactgg tcatggtttc acggcaagcc atcatctgtt
    11401 caattaacgg taacgtgaca ccagaaatca cttcacaacg ggagtgttta tggcttaata
    11461 atgaagccac gcggtacggt ggcgctccgg ctaaatcggt taagaagatg acaccctcac
    11521 cagagtccgc ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaatc
    11581 cgcgccagaa attcacggcc cggcactgaa gaagtgcccc atgttttttc tccagcgcat
    11641 ccagtatctc ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc
    11701 tccttagccg aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa
    11761 ctcctctccc ccgaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg
    11821 ttggatcagc gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa
    11881 ggtagtgacc agcggcacat catttatacc gaggaagccg tagcggaaac cactgatcat
    11941 ataaacgatt gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcaggga
    12001 gtaaaacacc ccgcccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat
    12061 gtcatcgaac gttttggcaa acacgccgtt cagcaaaccc gcaagggaga atagcaccgc
    12121 cgtgagcacc agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa
    12181 cagtgaaatt gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac
    12241 atatccggca ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg
    12301 ctggaacttg gcaccaaaaa atgatgacgc gacgttagcg taggcattgg tgatcaccga
    12361 catcatgatc agccccggta cgatgaactg catatagctg aagccatgca tatcgccaat
    12421 acgcgaacct atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg
    12481 caccagcgtc tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt
    12541 tagcgccacc cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa
    12601 acaaacagct cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc
    12661 tcacttaact gcgtaaatac gctgttgatc ccctgctcac gcagcacttc aacttccagc
    12721 gtcgcggtat cgaccagtcg atactgatag ccatcgagct tcggtaacgg acttttcggt
    12781 gcgagatcga gaataaaggt ttccgatttc agcttagcca gcagcgcctt catcgaggta
    12841 ttttccacca gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct
    12901 tccaggtagt gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag
    12961 ccccacattg agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt
    13021 agtttaggtt catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat
    13081 aacatacgcg cacgttcgtt gcgttttccc catagatcga gctgcttaag atatttttcg
    13141 ctgcggatgt acgcctcttt gcgctccaca ccgtagtacc ctgcctgatt caccacaatt
    13201 tgctgcacgg tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt
    13261 ttagcgttca cgacatcctt ctcgagatcg taaccaaata cgctgacccg cccggaggtt
    13321 ttatttacca gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg
    13381 agaagcgcat aaaaatcacc cgcttcgacc tgcaaatcta tcccacgaag cgcctgaacg
    13441 ccgcctggat aggttttttt aagctgttga agttccagtg caatggtcat aaatttttac
    13501 ttaccttacg ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta
    13561 gccaaacgta attatttggt tacaggtcgt taacctccat gaaagacata gatacactca
    13621 tcagcaacaa tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga
    13681 aactggcaca agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc
    13741 ctgcagaacg tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta
    13801 acctggtcat tcacactgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac
    13861 tcgaagttga acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag
    13921 ttgaaaaccc ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt
    13981 tcaaacatag ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg
    14041 aactgaacgt catggaacag gtgtataacc tgggccactc caccattatg caatcagcgt
    14101 ggaaacgcgg gcagaaagtt accattcacg gctgggccta cggcattcac gacggcttgc
    14161 tgcgtgatct ggatgttacc gccaccaacc gcgaaaccct tgagcaacgt taccgtcacg
    14221 ggatttccaa cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca
    14281 acacatccgg caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag
    14341 atgacggtaa cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga
    14401 gaaaccgata aattctaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt
    14461 acaaatcgcc agcgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt
    14521 attccccgag tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc
    14581 cagatctttg aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc
    14641 ggtcataaag tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat
    14701 aaatgagcca cgcagcagac ccaccagcac catatcgctg ccgctgtctt tgtaacgctc
    14761 agtaatctga cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac
    14821 ttctacagta tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg
    14881 caggttttgg caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc
    14941 gcggaatctg ttaataaagc tcgaattgtg atgacgatca cacatgttaa aacctataat
    15001 taattgctac taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac
    15061 aggctcgcga cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta
    15121 tctactcatt ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc
    15181 tggccttgtg atgctcggcg tcgcctggat gctgtggcgc agtaaacgtg ccgcgctttg
    15241 gctatacgcc gccctgctgc tcggcaccat gatttggggc gtctgggaag ttggtttcga
    15301 cttctgggcg ctgactccgc gcagcgatat tctggtcttc ttcggcatct ggctgatcct
    15361 gccgtttgtc tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt
    15421 ggcactgctg attagcggta gtatcctgac ctgggccgga tttaacgatc cgcaggaaat
    15481 caacggcacc ttaagcgccg atgccacacc tgcggaagct atctcacccg ttgccgatca
    15541 ggactggcct gcctatggtc gcaatcagga aggtcaacgc ttttcacctc tgaaacaaat
    15601 taacgccgat aacgtccata atctgaaaga agcctgggtg ttccgtactg gcgatgtgaa
    15661 gcagccgaac gatccgggcg aaatcaccaa tgaagtgacg ccgattaaag tgggcgacac
    15721 cctttacctg tgtaccgctc accagcgcct gtttgcgctc gatgccgcca gcggcaaaga
    15781 gaaatggcat tacgatcctg agctgaaaac taacgagtct ttccagcacg taacctgccg
    15841 tggcgtctct tatcatgaag ccaaagcaga aacggcttcg ccggaagtga tggcggattg
    15901 cccgcgtcgt atcattcttc cggtcaatga tggtcgactg attgcgatta acgctgaaaa
    15961 cggcaaactg tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc
    16021 agacaccaaa ccgggtctgt atgaaccgac ttcgccaccg attatcaccg ataaaaccat
    16081 cgtgatggca ggttcagtta ccgataactt ctcaacccgc gaaacgtctg gcgtgatccg
    16141 tggttttgat gtcaacaccg gggagctgct gtgggctttt gatcccggcg cgaaagatcc
    16201 gaacgcaatc ccgtctgacg aacacacctt tacctttaac tcgccaaact cgtgggcgcc
    16261 agcggcctat gacgcgaagc tggatctggt ctatctgccg atgggcgtga ccacgccgga
    16321 tatctggggc ggtaaccgca caccggaaca ggaacgttat gccagctcga ttctggcgct
    16381 gaatgccact accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga
    16441 catggatctt ccggcacagc cgacgctggc ggacatcacc gttaatggtc agaaagtgcc
    16501 agttatttac gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gcaatggcga
    16561 actggtggtt ccggccccgg aaaaaccggt gccgcaaggt gctgcgaaag gcgattacgt
    16621 tactccgacc cagccgttct ctgaactgag cttccgtccg aagaaagatt tgagcggtgc
    16681 ggatatgtgg ggagccacca tgtttgacca actggtgtgc cgcgtgatgt tccaccagat
    16741 gcgctatgaa ggcattttca cgccgccatc tgaacagggc acgctggtct tcccgggtaa
    16801 cctgggaatg tttgaatggg gcgggatttc cgttgatcca aatcgtgaag tggcaattgc
    16861 caacccaatg gcactgccgt ttgtttcgaa actgatccca cgcggtccgg gtaacccgat
    16921 ggagcagccg aaagatgcca aaggcacggg tacggaatcc ggcattcagc cacagtacgg
    16981 tgtaccgtat ggtgtcacgc tcaacccgtt cctctcaccg tttggtctgc cgtgtaaaca
    17041 gccagcatgg ggttatatct cggcgctgga tctgaaaacc aatgaagtgg tgtggaagaa
    17101 acgtattggt acgccgcaag acagtatgcc gttcccgatg cctgtaccgg tgccgttcaa
    17161 tatgggtatg ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc
    17221 tgctacggca gataactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca
    17281 gggccgttta ccagcgggtg gtcaggcgac gccaatgacc tatgaagtga atggcaagca
    17341 gtatgtggtg atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat
    17401 tgtggcttat gcgctgccgg atgatgtgaa ataaccataa tgcccggaga gatccgggca
    17461 tatttccgaa tacggtcttt ttataccgta aaccctaaca tcatccccgt atcttcatgc
    17521 tccagcagat ggcagtgcgc catataagca tgttctttcg gtgcgttgtg attaaacttc
    17581 accagcactt cactgacatt gccttctacc ttaacggtat ctttccagcc cgcgcgatga
    17641 gccgctggcg gtttgccatt ttctgacaag atacggaact gggtgccgtg aatatggaac
    17701 ggatgcagca tcatgtcgcc cacgccagag ataacccaac gttcgtactg ccctttcgcc
    17761 gccgcaaaca tcggcttatt catatcaaac gcctgaccgt tgattttatt ggcatggtgg
    17821 aaatcgaact tcccgccatg gttcatatga ttcatattgc cgtgccccat atggcccatc
    17881 atctggctat gatccatccc ggccatcgcc tgatcgccat atttctccat cagcatctgc
    17941 atccccatca tatcgagcat cgggtccata gaaagttgca gcttgcgtac cgtcagccct
    18001 tccagcgaag gtaacgcagg caggctactt aatgtgtctg gcaaagcacc ggaggcacta
    18061 atagcaatcg gctgaatccg cattaccgga tgcggcttgt caaacggcgc aatcgccatc
    18121 cccatctggc tgaccggtag cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc
    18181 agcacttcaa aacgctcgcc catcagcacc ggcagctcgc tcaccttcac cggttcaggt
    18241 agcagaccac cgtcgctggc aatcacatac agcgggcgat tgtcgctggt ggcgaaattg
    18301 agcgagcggg cattacagcc attaagcaaa cgcaggcgca gccaaccacg cggggcagcg
    18361 tgttgtgggt agattgcacc gttggtcagc agcgtatcgc caaaccagcc cacggcggcg
    18421 gtcatcacat ccagttgata atcaatctgc ccatcagcgt taaatttttt atcctgaaca
    18481 atcaccggaa catcatcgat gccccactgt tttggcagca ttaatttcag gatctcgtca
    18541 tcttcaatta ccaccagccc agccaacccc atcgccacct gtcgcccggt cttgccatgt
    18601 tgatgtggat ggaaccagca agtagcggca ggttgatcaa cgttcaacgt caccgagcgc
    18661 ttgccacctg gcagaataat tccctgcggg ccgccgtcga cttcacccgg tacttccagc
    18721 ccgtgccagt gcaacgtcgt ctcttccgtc agttggttat agatatcaac cgttaccgct
    18781 ttgccgcgct gtaatttcac cgccggcccc agcagattgc cgttatagcc ccaggtagtt
    18841 gcagttttct cgccaaaggt agactgacct gcgccaatag ttaactgaat gcgattacgg
    18901 gcatcggtcg tgagcaaatc agggatcggt aacgttgggc gttccgccgc aaagactgcg
    18961 cggctccaca gcggcaaggc tgaagccaca cccagcgcga cggaatattt taagaaatca
    19021 cgacgttgca tagttatttc cttattctta agcaggcgaa acaaaatcaa acgttgagca
    19081 tagtccttcc ccttacggga aggtcaagcc gcagacacaa tttaatcgac aataatggtc
    19141 gtcgcctcgc tcgcagtgtg ctaacgttta tcttctttaa gcccctggta gaagcaatga
    19201 agacgttttt cagaacagtg ttattcggca gcctgatggc cgtttgcgca aacagttacg
    19261 cgctcagcga gtctgaagcc gaagatatgg ccgatttaac ggcagttttt gtctttctga
    19321 agaacgattg tggttaccag aacttaccta acgggcaaat tcgtcgcgca ctggtctttt
    19381 tcgcccagca aaaccagtgg gatctcagta attacgacac cttcgacatg aaagccctcg
    19441 gtgaagacag ctaccgcgat ctcagcggca ttggcattcc cgtcgctaaa aagtgcaaag
    19501 ccctggcccg cgattcctta agcctgcttg cctacgtcaa ataatccctg ataccttttt
    19561 gtagaaatat tgaccgtgca tccgcggtca atgttagcta ttatgttgcg cccttttttt
    19621 acgggtgtta acaaaggagg tatcaaccca tggccgaaaa aaaacagtgg catgaaacgc
    19681 tacacgacca gtttgggcag tactttgcgg tagataacgt tctgtatcat gaaaagaccg
    19741 atcaccagga tctgatcatt tttgagaacg ctgcatttgg tcgcgtaatg gcgctggatg
    19801 gcgtagtaca aaccaccgag cgcgacgagt ttatctatca tgagatgatg acccatgttc
    19861 cgctactggc ccacggtcac gcgaaacatg tgctgattat cggcggcggc gacggtgcca
    19921 tgctgcgtga agtaacccga cataaaaacg ttgagtcaat cacgatggtg gaaatcgatg
    19981 cgggtgtcgt gtcgttctgc cgtcagtatc tgcccaacca taacgccggt agctacgacg
    20041 atccgcgctt taagctggtg atcgacgatg gcgtcaattt cgttaatcaa accagccaga
    20101 cctttgatgt cattatctcc gactgcaccg atcctatcgg tcccggcgaa agtctgttca
    20161 cctcagcatt ttatgaaggc tgcaaacgtt gcctgaatcc tggcggtatc ttcgtcgcac
    20221 aaaacggcgt ctgcttttta cagcaggaag aagccatcga cagccatcgc aaactcagcc
    20281 attacttcag cgacgttggc ttttatcagg cggcgatccc gacctattac ggcggtatca
    20341 tgacttttgc atgggcgaca gataacgacg ccttacgcca tctctcaacc gaaattattc
    20401 aggcgcgttt tctcgcttct ggcctgaaat gccgttatta caatccggca atccatacgg
    20461 cagcttttgc cttacctcag tatctgcaag acgcactggc ttcacagccg tcctaaggag
    20521 aagataagaa attgaaaaaa ctgaaactgc atggctttaa taatctgacc aaaagtctga
    20581 gtttttgtat ttacgatatc tgctacgcca aaactgccga agagcgcgac ggttatattg
    20641 cttatatcga tgaactctat aatgccaacc gtctgaccga aatcctgtca gaaacctgtt
    20701 ccattatcgg ggccaatatt cttaacatcg cccgccagga ttacgaacca cagggtgcca
    20761 gcgtcactat tctggtgagt gaagaaccgg ttgacccgaa actcatcgac aaaacagaac
    20821 accccggccc actgccagaa acggtcgttg cccatctcga taaaagtcat atttgcgtac
    20881 atacctaccc ggaaagtcat cctgaaggcg gtttatgtac cttccgcgcc gatattgaag
    20941 tctctacctg cggcgtgatt tctccgctga aggcgctgaa ttacctgatc caccagcttg
    21001 agtccgatat cgtaaccatt gattatcgcg tgcgcggttt tacccgcgac attaacggta
    21061 tgaagcactt tatcgaccat gagattaatt cgattcagaa ctttatgtct gacgatatga
    21121 aggcgctgta tgacatggtg gatgtgaacg tctatcagga aaatatcttc cataccaaga
    21181 tgttgcttaa agagttcgac cttaagcact acatgttcca caccaaaccg gaagatttaa
    21241 ccgacagcga gcgccaggaa attaccgctg cgctgtggaa agaaatgcgc gagatttatt
    21301 acgggcgcaa tatgccagct gtttaacggc tctggcggag ctcccaggct ccgccagatt
    21361 tatttacttc tgctgcacga aattgcggta agccgccacg acctgcagaa aatcctcaac
    21421 gccgcatagc gacaggcttt cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc
    21481 agcgaattcc agttgattgg cgcgaaccat cacctcttca ccgtccatcc acagggtgta
    21541 ttcatgccct gcccgttgcc aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc
    21601 cacttcatca agcaaggcca ggttttcttt cacctcttca ttaaaccagt gcccgaccac
    21661 ttcatgcccc atggacatac gcacctttac cactccggta atatcgcgca gaaattcgta
    21721 atccatagtg tgttcctctg ctcccggcaa tgcgccgaaa tcgcattgcg ccattgatgt
    21781 aattatcgca gccttaccgg agaaaaacag cggaacggcg atggtttaga aataaaaaat
    21841 gcccgggaat ctcattcacc gggcattgtg tcgtttatgc gcagcgcgtg cgcttactta
    21901 ttacaccgca gtctggaaaa tcaccccatc ggctttttcg gtgtactgag aaagctggtt
    21961 gaagttcaga taacgataag tatcaacggc agttttatct acttgcgcaa cgtaggtctg
    22021 gtactcttcc ggcgtcggca gtttgccaat cagcgccgca acagccgcca attctgcaga
    22081 agccaggaag acattcgcgc cagtccccag acggttcggg aagttacggg tagaggtgga
    22141 aaccaccgtc gcaccgtctg ctacacgcgc ctggttaccc atacacaggg aacagccagg
    22201 aatctcgata cgcgcaccgc tcttaccgaa gacgctatag tagccttctt cggtcaactg
    22261 cgcggcatcc atacgggttg gcggtgccac ccacaggcgc gtcggcaact ggcctttgtg
    22321 cgcatccagc agtttacccg cagcacggaa gtgaccgatg ttggtcatgc aggaaccgat
    22381 aaacacttcg tcgatcttct cgccctgtac cgcagacagc ggacgtgcgt cgtccgggtc
    22441 gttcggtgca cagaggattg gctctttaat atccgccaga tcgatgtcga tcactgccgc
    22501 gtattccgca tctgcatcgg cttccagcag ctcaggattc gccagccatt tttccatgcc
    22561 ctgaatacga cgttccaggg tacgacgatc gccgtaacct tccgcgatca tccacttcag
    22621 caggacgatg ttagagttca ggtattcgat gatcggttct ttgttcagct tgatggtaca
    22681 accagcggca gaacgctcgg cggacgcatc ggtcagttca aatgcctgct caactttcag
    22741 atccggcaga ccttcaattt ccagaatgcg accagagaag atgtttttct tgcctttctt
    22801 ctcaacggtc agcagacctt gtttgatcgc atacagcggg atcgcgtgta ccagatcgcg
    22861 cagggtgatg cccggctgca ttttgccttt gaagcgcacc agaacggatt ccggcatatc
    22921 cagcggcatt acgcctgttg cggctgcaaa tgccaccaga ccagaacccg ccgggaaaga
    22981 gataccgatc gggaaacggg tatgggagtc accaccggta ccgacggtat ccggcagcag
    23041 catacggttc agccaggagt ggattacgcc gtcacccgga cgcagcgaca caccaccacg
    23101 gttcataatg aagtccggca gcgtgtggtg cgtgttcacg tcaactggct tcggatacgc
    23161 cgcggtatgg cagaaagact gcatcaccag gtcagccgag aagcctaggc acgccaggtc
    23221 tttcagttca tcacgggtca tcgggccggt ggtgtcctgt gaacctacag aagtcatttt
    23281 cggttcgcag tacgcgcccg gacgaatacc tttcacaccg caggcacgac ctaccatttt
    23341 ctgcgccagc gagaagccgc gatcgctctc agcgacatct ttcgcctgac ggaacacatc
    23401 actgtgcggc agaccaagtg cttcacgcgc tttggtggtc aggccacgcc cgataatcag
    23461 cgggatacgg ccgccagcac gcacttcatc aatcagcacg tcggttttca gttcgaaggt
    23521 cgccagcagt tcgccggttt cgtggttacg cacttcacct ttgtacgggt aaacgtcaat
    23581 cacgtcgccc atgttcaggt tagagacgtc gacttcgatt ggcagtgcac ccgcgtcttc
    23641 catcgtgtta aagaagatgg gtgcaatttt accgccgagg cacaaaccac cgccgcgttt
    23701 gttcggcaca tgtggaatat catcgcccat aaaccacaga acggagttcg tggcggattt
    23761 acgcgatgaa cccgtaccca caacgtcacc gacgtacgcc agcgggaaac ctttctgttg
    23821 cagagcttca atctgtttga tcgggccaac aacacctggc tggtctggct caataccttc
    23881 acgggcgttt ttcagcatcg ccagcgcgtg cagtgggata tccgggcgcg accacgcatc
    23941 cggtgccgga gaaaggtcat cggtgttagt ttcgccagtg actttgaaga cagtaacggt
    24001 cagtttttca gccagcgccg ggcgattcag gaaccattcg gcatccgccc aggactgcat
    24061 aacctgcttc gcatattcgt tgcctgcttt cgctttctct tctacgtcat agaagttatc
    24121 gaacatcagc agcgtgtgag aaagtgcttt ggcagcaata ggtgccagtt tggcatcatc
    24181 cagcgcgtcg atcagcgaat gaatgttgta accaccctgc atggtgccca gcagttcgat
    24241 ggctttttcc ggagtcagca gaggggattt ggcttcgcct ttcgcgatag cagccaggaa
    24301 gcctgctttg acataggcgg cttcatcgac gcctggggga acacggttgg ttaacagatc
    24361 taacaggaat tcttcttcgc ccgcgggcgg gtttttcagc agctctacaa gtgcggccat
    24421 ttggtttgca tccaggggtt tgggcgcaat cccctcagcg gcacgctcag ctacgtgctt
    24481 acggtattct tctagcacga cggttctcct cgctctcatt gtcatagtgc ggcaggcgat
    24541 tctcttcacg ctcctgtgag acagcagttt gtagggtaaa tgcccagtac cgcaacgggc
    24601 agaatagcag gattttgacg atgtgttaat ctgtttacaa aaaagcaaca taaaaaagtg
    24661 gctgaatcgt taaggatggt ctagagattg tttccctccg taaaaacttc gttaattcgc
    24721 atggtgataa tcacctttct caacgaatcc caagattagt caaaatttaa actaccgcct
    24781 ctttatactc ggattcacag cacctgcggg tggcagttcg ccgaccattg cgatttcctt
    24841 gagatccgaa ttatgaaaat gactttgccg tttaaacccc atgtgctggc gctaatttgc
    24901 agtgccgggc tttgtgccgc ctctaccggg ctatatataa aaagccgcac agtggaagcg
    24961 cctgtggaac cgcaatcgac acaacagact gcgcctgaca tcaccgcagt tacgcttcct
    25021 gcaacggttt ccgcaccgcc cgtaacgccc gccgtcgtca aatccgcatt cagcactgca
    25081 caaatcgatc aatgggttgc gcctgtcgcg ctgtaccccg attctctgct ttcacaagtg
    25141 ttaatggcat caacctatcc ggcaaacgtt gctcaagcag tgcaatggtc gcacgataat
    25201 ccacttaaac aaggcgatgc tgctattcag gcggtatctg accagccgtg ggacgccagc
    25261 gttaaatcac tggtggcctt tccacaattg atggcattga tgggcgaaaa cccgcaatgg
    25321 gtgcaaaacc tgggcgatgc ttttctggcg cagccgcagg acgtgatgga ctcggtacag
    25381 cgattgcggc aactggcgca acaaaccggt tcgctgaagt catcaaccga acaaaaagtt
    25441 attaccacaa cgaagaaagc tgtaccggta aaacagacag tcacggcacc cgtcatacca
    25501 tccaataccg ttttaactgc cagccccgtc attacagagc ctgcaacaac cgtcatttcc
    25561 attgagcccg ccaatcctga tgtggtctat attcccaact acaacccaac cgtggtttac
    25621 gggaactggg ccaatactgc gtatccgccg gtttatctgc caccaccagc cggagaaccg
    25681 tttattgaca gctttgtgcg cggattcggc tatagcatgg gtgttgctac cacgtacgca
    25741 ctattcagca gcatcgactg ggatgacgac gatcatgacc atcatcatca tgacgatgat
    25801 gattatcatc accacgatgg cggtcatcgt gacggtaatg gctggcaaca caacggcgac
    25861 aacatcaata tcgacgtcaa caatttcaac cgtatcaccg gtgagcatct tactgataag
    25921 aatatggcat ggcggcacaa tccaaactac cgtaatggtg tgccctatca tgatcaggat
    25981 atggcaaagc ggtttcatca aaccgatgtc aacggcggaa tgagcgccac gcagctacct
    26041 gctccaacac gcgacagcca gcgtcaggcg gcagcaagtc agtttcagca acgaacacac
    26101 gccgcccccg tcattacacg agatacccaa cgtcaggcag cggcacagcg gtttaatgaa
    26161 gctgaaaact atgggagcta tgacgacttc cgcgacttca gccgtcgcca accactgacc
    26221 cagcaacaaa aggacgccgc tcgtcagcgt tatcagtcgg cctcgcctga gcagcgccag
    26281 gcagtccgcg agaaaatgca gactaaccca cagatccagc agcgaagaga cgcagcgcgt
    26341 gagcgtattc agtcagcctc gcctgagcag cgccaggtgt ttaaggaaaa agtacagcag
    26401 cgcccactga accaacagca acgtgataac gcccgccagc gtgttcaatc agcatcacct
    26461 gaacaacgtc aggtttttcg ggaaaaagtt caggagagcc gcccacaacg tctaaacgac
    26521 agtaaccata ctgtcaggct gaataacgag caacggtcag cagtacgcga acgtctctct
    26581 gagcgcggag caaggcgaca ggaaaggtaa attgcagacg taaaaaaagc ggcgtggtta
    26641 gccgcttttt taattgccgg atgttccggc aaacgaacaa ttacttcttc ttcgctttcg
    26701 ggttcggcag gtcggtaatg ctaccttcga acacttctgc cgccaggccc acagactcgt
    26761 gcagagtcgg gtgcgcgtgg atggtcagtg cgatgtcttc agcatcacaa cccatttcga
    26821 ttgccaggcc gatttcaccc agcagctcgc cgccgttggt accgacaatc gcaccaccga
    26881 tcacacggtg agattctttg tcgaaaatca gcttggtcat accgtctgcg cagtcggaag
    26941 cgatagcacg accagaagca gcccacggga aggtggcggt ttcatagctg atgcctttct
    27001 ctttcgcttc tttctcagtc agacctaccc atgcaacttc tggttcggta taggcgatgg
    27061 acgggataac tttcggatcg aagtagtgtt tcttaccggc gataacttca gcggcaacgt
    27121 gaccttcgtg aacacctttg tgtgccagca tcggctgacc gacgatatcg ccgatagcaa
    27181 agatgtgcgg tacgttggta cgcagctgtt tgtcaacgcg gatgaaacca cggtcgtcca
    27241 cttccacgcc agctttgcct gcgtcgaggt ttttaccgtt cggcacacga ccaatcgcta
    27301 ccagcacggc gtcgtaacgc tgcggttcag cgggtgcttt tttgccttcc atcgtcacat
    27361 aaataccgtc ttctttcgct tcaacggcgg taactttggt ttccagcatc aggttgaatt
    27421 tcttgctgat acgcttggtg aagactttaa cgatgtcttt gtcagctgcc gggataacct
    27481 ggtcgaacat ttcaaccacg tcaatctgtg aacccagcgc gtggtatacg gtgcccattt
    27541 ccagaccgat gataccgcca cccattacca gcaggcgttc tggtacttct ttcagttcca
    27601 gcgcgtcagt ggagtcccag atacgcggat cttcatgcgg aataaacggc agttggatcg
    27661 ggcgagaacc cgctgcaatg atcgcattgt cgaagttgat cacggttttg ccgttctcac
    27721 cttcaacttc cagggtgtta gccccggtga atttacccag accgttgacc actttgactt
    27781 tgcggccttt cgccatacca gccagaccac cggtcagctg attgatcact ttctctttcc
    27841 aggtacgaat cttgtcaata tcggttttcg gttcgccgaa gacgataccg tgttcagcca
    27901 gcgctttggc ttcttcgata acttttgcta cgtgcagcag tgctttagaa gggatacagc
    27961 cgacgttcag gcaaacaccg ccaagggtgt tgtaacgttc tacgattacg gtttccagac
    28021 ctaaatcagc gcaacggaag gcagcggagt aacctgcggg gcctgcccca agtaccacga
    28081 cctgagtttt gatttcagta ctcatcatga cctctatata tttatctccg gcggtcatac
    28141 ccgtcgtctt tcaggccgca gcggcgttag cttcacgccc tcaccccagt cacttactta
    28201 tgtaagctcc tggggattct ggcgcttgct accttgctgc acccagaaat ccatagggta
    28261 tgtgaagtaa taccctaacc accaccgggt cgttctatcc gtcggtattt tacaaaattg
    28321 ttaacaattt ttaaacaaca aacggcaacc gatttgtcta ttcgctaata acctcaatac
    28381 attcatgaga ttaccagaaa aaagccggcc gttgggccgg ctcttttact tacatcacca
    28441 gacggcgaat gtcagacagc gtgttgttaa tgatggtaat gaaacgggca ccatcagcac
    28501 cgtcgatcac gcggtggtcg aaggagagag aaatcggcag catcagacgc ggcacgaact
    28561 ctttaccatt ccacaccggc tccatcgcgg acttggaaac gccgaggata gccacttccg
    28621 gcgcgttcac aatcggcgcg aagtgggtag tacccaggcc gccgatgctg gagatggtga
    28681 agcaaccgcc ctgcatttcg cccgcagtca acttaccgtc acgcgctttc ttagaaatag
    28741 tcatcagctc gcgagacagc tcgatgatgc ctttcttgtt gacgtctttg aataccggaa
    28801 caaccagacc gttcggggta tccaccgcca caccgatgtt gatgtatttc ttcagggtca
    28861 gacgctgacc gtcttccgac aacgaactgt tgaagcgagg catctgctca agagctgcag
    28921 caacggcttt catgatgaag acaaccgggg tgatcttcac atccagctta cgtttcgccg
    28981 cttcttcgtt ctgctgttta cggaacgctt ccagctcggt gatatcggtt ttgtcgaagt
    29041 gagtaacatg cgggatcatc acccagttac ggctcaggtt cgcaccagaa attttctgga
    29101 tacggcccag ttccacttct tcgatttcac caaacttgct gaagtccacc ttcggccacg
    29161 gcagcatgcc tgggataccg ccgccagtcg ccgccggagc tgcttctgca cgtttgatag
    29221 cttctttcac gtaagcctga acgtcttcgc gcaggatacg acctttacgg ccagtgccct
    29281 tcactttcgc caggttaacg ccaaactcgc gtgccagacg gcggatcagc ggagtcgcgt
    29341 gaacgtaagc gtcgttttct gcaaattcag atttgccttc cgctttcgca gccggtgctg
    29401 ctgccggagc ttcagctttt gctgccggag ccggcgctgc cgcttcctgt ttcgcaggag
    29461 ccgccgcagg cgctgcgcct tcaacttcga agatcataat cagcgagcca gttttcactt
    29521 tatcgccaac gttgactttc agttccttca cgacgcctgc aaacggagcc ggaacttcca
    29581 tagaagcttt gtcgccttct acggtaatca gtgactgttc agcggcaact ttgtcgccca
    29641 ctttcaccat cacttcggtc acttcaactt cgtcaccgcc gatatccgga acgttaactt
    29701 ctttcacgcc agccgctggt gcaggggccg ctgccggagc cgcttcctgt ttagccgccg
    29761 gagctgccgc gcctgcttca cccgcgactt cgaagaccat aatcagcgag ccggtagaca
    29821 ctttgtcacc cacgttcact ttgatctctt tcacggtgcc agcaaacgga gccggaactt
    29881 ccatagacgc tttatcgcct tctacggtga tcagcgactg ttcagcttca actttatcgc
    29941 caactttcac caggatttcg gtcacttcaa cttcgtcgct gccgatatcc ggaacgttaa
    30001 cgtcttttgc cgctgcagcc gctggtgctg ctgccggagc tgcttctttc ttctcttctg
    30061 cctgagcagg tgcagcgtct gctgcaccgt cggcggaatc gaaaatcata atcagtgcgc
    30121 cggtctgggt tttatcgcca acagagactt tgatctcttt aacgataccc gcctgcggag
    30181 acggaacttc catagaggct ttgtcgcctt ctacggtgat cagcgactgt tcggcttcaa
    30241 ctttgtcgcc cactttgacc aggatctcgg tgatttcaac ttcatcagcc ccgatgtccg
    30301 gtactttgat ttcgatagcc attattcttt tacctcttac gccagacgcg ggttaacttt
    30361 atctgcatcg atgttgaatt tggcgattgc gtcagcaacc actttcttat cgatttcgcc
    30421 acgtttagcc agttcgccca gcgccgctac aaccacgtag gaagcatcaa cttcgaagtg
    30481 gtgacgcagg ttctcacggc tgtcggaacg accgaagcca tcagtaccca gtacgcggta
    30541 gtcgtcagcc ggtacgtaag tacggacctg ctcagcgaac agtttcatat agtcggtaga
    30601 tgccactgcc ggagcgtcgt tcatcacctg agcgatatac ggaacgcgcg gagtttccag
    30661 cgggtgcagc atgttccagc gttcacaatc ctgaccatct cgcgccagtt cggtgaagga
    30721 ggtcacgcta taaacgtcag aacctacgcc gtaatctttc gccaggatct ctgccgcttc
    30781 acggacgtga cgcaggatag aaccggagcc cagcagctga actttacctt tgctaccttc
    30841 aatggtttcg agtttgtaga tacctttacg gataccttcc tcagcacctt ccggcattgc
    30901 cggcatgtgg tagttttcgt tcagcgtggt gatgtagtag taaacgttct cttgtttttc
    30961 accgtacata cgctccagac cgtcatgcat aatgacagca acttcgtaag cgtaagccgg
    31021 gtcgtaagag atacagttcg gaatagtcag cgactgaatg tggctgtgac catcttcgtg
    31081 ctgcagacct tcgccgttca gggtggtacg accggaagta ccgccgatca ggaagccacg
    31141 cgcttgctgg tcgccagccg cccagcacag atcgccaata cgctggaagc cgaacatcga
    31201 gtaatagatg tagaacggga tcatcggcag attgttggtg ctgtaagagg tcgccgctgc
    31261 cagccaggaa caacctgcgc ccagctcgtt gatcccttcc tgcagaatct gacctttctc
    31321 gtcttcttta tagtaagcaa cctgctcgcg gtcctgcggg gtgtactgct gaccgttcgg
    31381 gctgtaaata ccaatctgac ggaacagacc ttccataccg aaagtacgcg cttcgtcggc
    31441 gatgatcggt accagacgat ctttgatcga cttgttcttc agcattacgt tcagggcacg
    31501 aacgaaagcg atagtggtag agatctcttt gctctgctct tccagcagcg cgccgaagtc
    31561 ttgcaggctc ggcagctcaa gcttctcggt gaagttcggc tgacggcttg gcagataacc
    31621 gtgcagtttc tgacgctgag cgtgcagata ggtatgctct tcagaacctt ccgggaaggt
    31681 gatgtacggc agtttttcga tatctgcatc agacaccggc acattgaaac ggtcgcggat
    31741 gtgacgcacg ccgtccatgt tcattttctt aacctggtgc gcgatgtttt taccttcagc
    31801 cgcgtcgccc atgccgtaac ctttaatggt atgagcaagg attactgtcg ctttgccttt
    31861 ggtttcctgc gctttcttga atgcagcgta gattttcttc ggatcgtgac caccacggtt
    31921 cagtgcccag atctgctcgt cagtccagtc tgcaaccagt gctgcggttt caggatattt
    31981 accgaagaag tgttcacgaa cgtacgcacc atctttcgat ttgaaggtct ggtagtcgcc
    32041 gtcaacggtt tcgttcatca gctggatcag tttaccgctg gtatctttac gcagcagttc
    32101 atcccaacgg ctaccccaca tcactttgat cacgttccag ccagcacctt cgaagatgcc
    32161 ttccagttcg ttgatgatct tgccgttacc ggtgaccggg ccgtcaagac gctgcaggtt
    32221 acagttgata acgaagacta ggttatccag tttttcacgg gtagcgatgg tgatcgcacc
    32281 tttggattcc ggttcgtcca tttcaccgtc gccgaggaac gcgtaaacgg tctgtttaga
    32341 ggtatctttc aggccacggt gttccagata tttcaggaat ttagcctggt aaatagcacc
    32401 aatcggcccc agacccatag atacggtcgg gaactgccag aattccggca tcagtttcgg
    32461 gtgcggatag gaagagaggc cattgccgtg aacttcctga cggaagttat ccagctgctc
    32521 ctgagtcaga cgaccttcta ggaaagcacg agcgtacacg cccggggaga tgtggccctg
    32581 gaagtaaacc aggtcgccgc catcctgctc gttgcgtgca cggaagaagt ggttaaagca
    32641 cacatcataa atggttgcgg aagactggaa ggacgccata tggccgccca gttcgaggtc
    32701 ttttttcgac gcacgcagca ccgtcatgat ggcgttccag cggatagctg aacgaatacg
    32761 gcgttccagt tccagattac ccggatactc cggttgttct tcaacgggga tggtgttgat
    32821 gtagttgctg atacctgtgc ctgcggctac gtttacaccg cctttgcggg cttcagcaag
    32881 cagttggtcg atcagatact gagcacgctc aacaccttct tcacggatga ccgattcgat
    32941 cgcctggagc cagtcgcgag tttcgatcgg atccacgtca tttgggaaac gttctgacat
    33001 gggttattcc ttatctatct aataacgttg agttttctgg aacctgtccc attgaactct
    33061 cgccggaaag ctcaataaga caggttctac gtttagttgc cgcgctttta tatgcgcttg
    33121 atttacaaca tcttctggat aatttttacc agaaaaatca ctaattcttt cgttgctcca
    33181 gacgacgcag agaacgctca cggcggctct cttcacgact tctgtcgagc aaaatttctt
    33241 cgataaaggc cagatggcga tgcgatgctt cgcgcgcttc ttccggctta ccggccataa
    33301 tcgcttcaaa tatgcgggtg cggtgactac tcaccagcgg cagcatctcg cgacgcgaat
    33361 agagcaattc gaagttctgg cggacattct gggccaacat cggctccata caccttagca
    33421 gatgaagcag aaccacattg tgggccgctt cagtgacagc aatctgatac tggagtacgg
    33481 cgtttgattc cgcgtccaga tcgccagact gctgcgccag ctctatggcg tggtggagtt
    33541 cacggatgcg ttccttgtct tcatcggtac tacgcagcgc ggcgtaataa gcggcgatac
    33601 cttccagggc gtgtcgtgtt tcgagcaagt catactgtga ctcaggatgg tcggagagca
    33661 gctccaccag cggatcgctg aagctttgcc ataggctgct ctggacaaaa gtgccgccac
    33721 cctggcgacg aagcaacaag cccttcgctt cgagacgttg aatcgcctca cgcaaggagg
    33781 gacgggagac gtcaaactgt tttgccagtt cgcgttccgg tgggagtttt tcgcccgggc
    33841 ggagagtgcc ttcgaggatc aaaaactcca gttgctgctc aatcacatcg gagagttttg
    33901 gttggcggat tttgctgtag gccatgagtt cctgtcttaa gccacttgcc gaagtcaatt
    33961 ggtcttacca atttcatgtc tgtgacgcta aagtaacaaa gtattcacct tatgtccata
    34021 caggttttga ttgaaatcat gaaactgtgc acattttaac aacttgacat atataacgtt
    34081 tcaaagttgt aactatgcac aaatgtagac tttacgtagg aaaggagttt ttta
//
LOCUS       DAZFKM010000037        33946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFKM010000037 DAZFKM010000000
VERSION     DAZFKM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(142..894)
                     /gene="tcyN"
                     /locus_tag="ACHKAY_001927"
     CDS             complement(142..894)
                     /gene="tcyN"
                     /locus_tag="ACHKAY_001927"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO5700608.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(891..1559)
                     /gene="tcyL"
                     /locus_tag="ACHKAY_001928"
     CDS             complement(891..1559)
                     /gene="tcyL"
                     /locus_tag="ACHKAY_001928"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO5700609.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1574..2560)
                     /gene="dcyD"
                     /locus_tag="ACHKAY_001929"
     CDS             complement(1574..2560)
                     /gene="dcyD"
                     /locus_tag="ACHKAY_001929"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO5700610.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2665..3465)
                     /gene="tcyJ"
                     /locus_tag="ACHKAY_001930"
     CDS             complement(2665..3465)
                     /gene="tcyJ"
                     /locus_tag="ACHKAY_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5700611.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3553..4104)
                     /gene="fliZ"
                     /locus_tag="ACHKAY_001931"
     CDS             complement(3553..4104)
                     /gene="fliZ"
                     /locus_tag="ACHKAY_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO5700612.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4150..4869)
                     /gene="fliA"
                     /locus_tag="ACHKAY_001932"
     CDS             complement(4150..4869)
                     /gene="fliA"
                     /locus_tag="ACHKAY_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO5700613.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5034..6497)
                     /locus_tag="ACHKAY_001933"
     CDS             complement(5034..6497)
                     /locus_tag="ACHKAY_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO5700614.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6752..8164
                     /gene="fliD"
                     /locus_tag="ACHKAY_001934"
     CDS             6752..8164
                     /gene="fliD"
                     /locus_tag="ACHKAY_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO5700615.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8179..8589
                     /gene="fliS"
                     /locus_tag="ACHKAY_001935"
     CDS             8179..8589
                     /gene="fliS"
                     /locus_tag="ACHKAY_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO5700616.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8589..8954
                     /gene="fliT"
                     /locus_tag="ACHKAY_001936"
     CDS             8589..8954
                     /gene="fliT"
                     /locus_tag="ACHKAY_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO5700617.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9032..10519
                     /gene="amyA"
                     /locus_tag="ACHKAY_001937"
     CDS             9032..10519
                     /gene="amyA"
                     /locus_tag="ACHKAY_001937"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5700618.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10553..10966)
                     /gene="yedD"
                     /locus_tag="ACHKAY_001938"
     CDS             complement(10553..10966)
                     /gene="yedD"
                     /locus_tag="ACHKAY_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO5700619.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11153..12358
                     /gene="yedE"
                     /locus_tag="ACHKAY_001939"
     CDS             11153..12358
                     /gene="yedE"
                     /locus_tag="ACHKAY_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO5700620.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12355..12588
                     /gene="yedF"
                     /locus_tag="ACHKAY_001940"
     CDS             12355..12588
                     /gene="yedF"
                     /locus_tag="ACHKAY_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO5700621.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12695..13363
                     /gene="yedK"
                     /locus_tag="ACHKAY_001941"
     CDS             12695..13363
                     /gene="yedK"
                     /locus_tag="ACHKAY_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO5700622.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(13758..14072)
                     /gene="fliE"
                     /locus_tag="ACHKAY_001942"
     CDS             complement(13758..14072)
                     /gene="fliE"
                     /locus_tag="ACHKAY_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO5700623.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14287..15945
                     /gene="fliF"
                     /locus_tag="ACHKAY_001943"
     CDS             14287..15945
                     /gene="fliF"
                     /locus_tag="ACHKAY_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO5700624.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15938..16933
                     /gene="fliG"
                     /locus_tag="ACHKAY_001944"
     CDS             15938..16933
                     /gene="fliG"
                     /locus_tag="ACHKAY_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO5700625.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16926..17612
                     /gene="fliH"
                     /locus_tag="ACHKAY_001945"
     CDS             16926..17612
                     /gene="fliH"
                     /locus_tag="ACHKAY_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO5700626.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17612..18985
                     /gene="fliI"
                     /locus_tag="ACHKAY_001946"
     CDS             17612..18985
                     /gene="fliI"
                     /locus_tag="ACHKAY_001946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO5700627.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            19004..19447
                     /gene="fliJ"
                     /locus_tag="ACHKAY_001947"
     CDS             19004..19447
                     /gene="fliJ"
                     /locus_tag="ACHKAY_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO5700628.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19444..20571
                     /gene="fliK"
                     /locus_tag="ACHKAY_001948"
     CDS             19444..20571
                     /gene="fliK"
                     /locus_tag="ACHKAY_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO5700629.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            20676..21140
                     /gene="fliL"
                     /locus_tag="ACHKAY_001949"
     CDS             20676..21140
                     /gene="fliL"
                     /locus_tag="ACHKAY_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO5700630.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21145..22149
                     /gene="fliM"
                     /locus_tag="ACHKAY_001950"
     CDS             21145..22149
                     /gene="fliM"
                     /locus_tag="ACHKAY_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO5700631.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22146..22559
                     /gene="fliN"
                     /locus_tag="ACHKAY_001951"
     CDS             22146..22559
                     /gene="fliN"
                     /locus_tag="ACHKAY_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO5700632.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22562..22927
                     /gene="fliO"
                     /locus_tag="ACHKAY_001952"
     CDS             22562..22927
                     /gene="fliO"
                     /locus_tag="ACHKAY_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO5700633.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22927..23664
                     /gene="fliP"
                     /locus_tag="ACHKAY_001953"
     CDS             22927..23664
                     /gene="fliP"
                     /locus_tag="ACHKAY_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO5700634.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23674..23943
                     /gene="fliQ"
                     /locus_tag="ACHKAY_001954"
     CDS             23674..23943
                     /gene="fliQ"
                     /locus_tag="ACHKAY_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO5700635.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23952..24737
                     /gene="fliR"
                     /locus_tag="ACHKAY_001955"
     CDS             23952..24737
                     /gene="fliR"
                     /locus_tag="ACHKAY_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO5700636.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25027..25650
                     /gene="rcsA"
                     /locus_tag="ACHKAY_001956"
     CDS             25027..25650
                     /gene="rcsA"
                     /locus_tag="ACHKAY_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO5700637.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25694..25882)
                     /gene="dsrB"
                     /locus_tag="ACHKAY_001957"
     CDS             complement(25694..25882)
                     /gene="dsrB"
                     /locus_tag="ACHKAY_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO5700638.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26045..26272
                     /gene="yodD"
                     /locus_tag="ACHKAY_001958"
     CDS             26045..26272
                     /gene="yodD"
                     /locus_tag="ACHKAY_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO5700639.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26570..27385
                     /gene="yedP"
                     /locus_tag="ACHKAY_001959"
     CDS             26570..27385
                     /gene="yedP"
                     /locus_tag="ACHKAY_001959"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO5700640.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(27382..29076)
                     /gene="dgcQ"
                     /locus_tag="ACHKAY_001960"
     CDS             complement(27382..29076)
                     /gene="dgcQ"
                     /locus_tag="ACHKAY_001960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO5700641.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(29314..29496)
                     /gene="yodC"
                     /locus_tag="ACHKAY_001961"
     CDS             complement(29314..29496)
                     /gene="yodC"
                     /locus_tag="ACHKAY_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO5700642.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29575..30492)
                     /gene="yedI"
                     /locus_tag="ACHKAY_001962"
     CDS             complement(29575..30492)
                     /gene="yedI"
                     /locus_tag="ACHKAY_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO5700643.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            30665..31585
                     /gene="yedA"
                     /locus_tag="ACHKAY_001963"
     CDS             30665..31585
                     /gene="yedA"
                     /locus_tag="ACHKAY_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO5700644.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(31574..32044)
                     /gene="vsr"
                     /locus_tag="ACHKAY_001964"
     CDS             complement(31574..32044)
                     /gene="vsr"
                     /locus_tag="ACHKAY_001964"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO5700645.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(32025..33443)
                     /gene="dcm"
                     /locus_tag="ACHKAY_001965"
     CDS             complement(32025..33443)
                     /gene="dcm"
                     /locus_tag="ACHKAY_001965"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO5700646.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33510..>33946)
                     /locus_tag="ACHKAY_001966"
     CDS             complement(33510..>33946)
                     /locus_tag="ACHKAY_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO5700647.1"
                     /translation="EFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEA
                     LGAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTAR
                     GKQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT         8287 a         8808 c         8609 g         8242 t
ORIGIN      
        1 attcaggcaa tgaattactt ttgcaagcca tcacattctc ttatgttatt aatgagttat
       61 gctgatttgt taagcagttt tatcacgctt gaaatagcgt cccgccccga caggtgaatc
      121 gtcggggctg attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg
      181 aggctgctgg ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgcccctg
      241 gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat
      301 caccatcgtg cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag
      361 ctctggatcc agcgccgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag
      421 cgcacgtgca atcgcaacac gttgctgttg accgccagac aaacgacgtg gatagctggt
      481 ttctttaccg gccagcccaa ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc
      541 tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg
      601 cggaaacaaa ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag
      661 cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt
      721 tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc
      781 actcggaccg ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag
      841 caccgtctga ccgtgaaatt ttttcaccaa gttcttaact tcaatggcac tcattttggt
      901 tctctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc
      961 gccatgatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc
     1021 gaggtaatca actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac
     1081 gtatccttta ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc
     1141 tgtggcaaaa tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct
     1201 tcccactgac ctttatcaat agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg
     1261 gcagtgttca gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac
     1321 tgcggcaggc cgtaatagat cataaacagt tgtgcgatga gcggtgtacc acgaaaaata
     1381 gagatataaa aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg
     1441 agaataaacc cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac
     1501 ccagcccctt tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg
     1561 agcgttcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat
     1621 gaataaacag aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagcc
     1681 ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca
     1741 atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat
     1801 cccagagtat aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta
     1861 ggttaaccac tttcggcaac tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt
     1921 cgctttcagg catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc
     1981 ccgatgcgac taccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca
     2041 gcgcactctc cacataacct agcgcgccaa gagcattaga accgccaacc ggaatgacat
     2101 acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat
     2161 cggtcagtgc ttcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc
     2221 cgttggttaa atagttttct gcggttgtgc caataggatt ttccagcagc gccacacagt
     2281 gcagaccgag tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgccc
     2341 cggcagtaat cagcgtatcg gcaccttcgc gcagagcatc tgctgcgaga aattccagct
     2401 tacgtaattt attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt
     2461 cccgtccaag ataatcagaa aagcgcggca gatattcgag cggtgttggc gcgccgataa
     2521 actccaaccg tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt
     2581 gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctggtgaaag
     2641 cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga
     2701 aagggcttgc agagtgccat ctttttgcat ttccgcaatt gcatcgttca ctgctttcag
     2761 caggtcttca tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc
     2821 ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac
     2881 gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata
     2941 ggtacgcaca tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag
     3001 accgacaccc acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc
     3061 ttttttcacc agcgcctgaa taccagaaat ggtgtacggg gtagagaaat cgtatttttt
     3121 cttgcgctca tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagagtccag
     3181 cgacgccagc ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt
     3241 tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa
     3301 actgaacggc ggataagtgc cttccagccc taccagcagc gtgccgcgct ctttaacttt
     3361 attaagcaga ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgctacggc
     3421 catcacactc atcaatgcct gacgtcccag atgtgctaat ttcatattta ccccgaatgt
     3481 tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca
     3541 tcttattctt atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc
     3601 ggtacacgtt tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt
     3661 gctggtggtg ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagcggatc
     3721 gagggaaata ttttgctcgt gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc
     3781 acgcaccgta cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat
     3841 atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc
     3901 ccatgatttt tgttccgttt gccagccatt ttcatcaagc agcgcgtcca ggcgggaaat
     3961 ctcgatttta ttcactattt tgccgtcgcg aacgagggta atgcgatcga gtaatttacg
     4021 gcaatgcgcg caatgagtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg
     4081 tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat
     4141 ttactgacgt tataacttac ccagtttagt gcgtaaccgt ttaatagcct ggctgtgtaa
     4201 ctgactgacc cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc
     4261 ttcctggtaa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatggcttc
     4321 catcacccgc tgacgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg
     4381 atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa
     4441 gagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa
     4501 acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat
     4561 tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg
     4621 aagttcatcc agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc
     4681 ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag
     4741 atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac
     4801 cagcgggaca taacgctgcc atagcgagtg tttatccatt acaccttcag cggtatagag
     4861 tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc
     4921 agggttttta atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa
     4981 attgcgggca gaaaaaaccc cgccggtggc gggggttgag cgataagtgt aaattaacgc
     5041 agcagagaca gtacgttctg cgtggtctgg ttagcctgcg ccagaacaga ggtacccgct
     5101 tgttgcagga tctgcgcacg agacatgtta gacacttcgg tcgcgtagtc agcatcttcg
     5161 atacggctac gggcagaaga caggttgttt acggtgttgc caaggttggt gatagcagag
     5221 tcgaaacggt tttgtactgc accgaggtca gaacgcagat tgtcaacttt agccaatgct
     5281 ttgtcgatag tttcgagcgg gttggtggta gattgcaacg attttgctgc atcttcgttt
     5341 accagaatcg ggctaccacc ttctgatttg ctcagataca tgactttatt gttaccagaa
     5401 gcagtctccg ttatcgtttt accatctgca ctaacatcgt aagttgcacc gttaacaact
     5461 aacgtgcttc ctgttttctt agctgcattg agatcaagat cggaaagtgt ggcagccttg
     5521 ttatcaactt tggttgtcgt ataaaggcca gcgctatttt tgtaaactgt ctcactcgta
     5581 atcaaagaag cttttttgtc agcatctgtc acagtaaatg tcactgactt accatcaatt
     5641 tctgcagaat atacaccatt cccattgcca tccgctgcag ttgttgttgt gaattcaact
     5701 ccattaaacg tgaatttatc attctgtgct ttattcttag ccgctgctgc gagtgctgta
     5761 gcatcaatcc ctgtaccagc tatgtttgtg tcagatttag ttgtcagtga accatctgct
     5821 gcactaacaa aaatatcatt ccctgtagta tctttaacag ctccgctatc tacgttaaca
     5881 gtataagcat caccgccaac atcatagtta tcagtacctg tcgctttaaa tttagaaatc
     5941 aggtcactgc cagttgcttt ctgcgcgcca tcgatattaa aaccgtccag gccgagagtt
     6001 ttcgcatcaa tttttgccag attgatagtg atggtttcac catcattagc accaacctga
     6061 attttcattt cattattttc agcaaggact ttcacgccgt taaactgagt ttgctcagat
     6121 acacggtcaa tttcttccag acgttgagta atttcagcct ggatagaaga aagatcgctg
     6181 tcagagttag taccgttagt tgcctgaaca gtcagttcac gtacacgctg caggttgttg
     6241 ttaatttcat tcagcgcacc ttcagtggtc tgcgcaacag aaataccatc attcgcgtta
     6301 cgggaagcct gagtcagacc tttaatattt gctgtaaaac ggttagcaat cgcctgacct
     6361 gctgcgtcat ctttagcact gttaatacgc aggccagaag agagacgttc aatggcggag
     6421 ctcagagaag actgagattt gttcaggtta ttctgggtca acagcgacag gctgtttgta
     6481 ttaatgactt gtgccatgat cttttcctta tcaattacaa cttgatgtta ttgggctgtt
     6541 gcccacggtt tctcaccgta ccccttgtat cggcacctga atttcgaact ttagaaaatt
     6601 ttagtttctc tcctgttttt tcttaacggc tgaaatatcc gtaatataca actctactct
     6661 gcgcatcatc ttcgtgaatc aactattaaa ctttgcgcac aaattgccga taacccgctt
     6721 atctactgtt tgcaatcaaa aggaattagg tatggcttca tttacatctc ttggcgttgg
     6781 ttcgaatctg ccgctggata cattgctaaa caacctgacc atcgctgaaa aaaaacgctt
     6841 aaacccaatt actcagcaac agagtgacaa taccgcacgt ctgacagcct atggcacatt
     6901 aaaaagtgca ttagaaaaat tccagactgc aaatactgcg ttaaataaag ccgatctgtt
     6961 caaaagcact accgttacca gtagcaatga agaccttaaa gtttccaccg aagcaggtgc
     7021 tgcccccggt atctatacca tcagcgtaac ccaactggcg caggcgcaat ctttacgaac
     7081 tgattccccg acaatcattg catcaacaaa agatgcactg ggtgatgagt catcagatac
     7141 acgcacaata aaaattaccc aagacggtcg taaagagccg ctggaaatca agctcaataa
     7201 agatcagacg tcattggatg agatcagcaa agccattaac gatgccgata gtggaatttc
     7261 cgctagtatc gttaaagtca aagatggtaa ctatcaattg gtattgaccg ccagtgaagg
     7321 tctggccaat aaaatgacca tatctgttga gggtgacagt aaattaaatg atctgctggc
     7381 ttatgacagc aagaccaaca ccgggaatat gaaggaactg gttaacgcgc aaaatgcaca
     7441 gctaaacgtt aacggtatcg atattgaacg ttcaagtaac aaaattaccg atgctcctca
     7501 gggagtgact ctcgatttga ccaaaaaagt cactgacgtc cgggtcacgg tgacaaaaag
     7561 caacgataaa gcaaccgaag caattaaagg ctgggtggat tcatataact ccctcattga
     7621 cacctttaac accctgacaa aatataaaga agtagaccca ggtgctgaag cacaagataa
     7681 aaataatggt gcacttctcg gagacagcgt tgtacgtaca attcaaagcg gcatccgcgc
     7741 acaatttgca aacggtgcca gcgacagtgc cttcaaaaca ttaaacgaga tcgggattaa
     7801 gcaggacggc acgacgggta agctgaaaat tgatgatgat aaactcaaaa aagtcctgaa
     7861 tgaaaataca gcatccgtac gtgaattgct ggtaggcgat ggtaaagaaa caggaattac
     7921 caccaaaatt gccaccgagg taaaaggtta ccttgccgat gatggcatta ttgatagcgc
     7981 acaggacagt attaacgcca cactgaaaaa actgaccaaa cagtatctat ccgtcagtgc
     8041 cagcattgat gacaccgtgg cgcgttacac tgcacagttt acgcaattag ataccatgat
     8101 gagcaaactg aataacacca gtacctatct cagccaacaa tttactgcaa tgagtaattc
     8161 ctgatcacgg aggtcagcat gtataccgcg agcggctcaa aagcctatgc acaaattggc
     8221 gtcgaaagcg ccgtaatgag cgccagccag cagcagctgg tcaccatgct atttgatgga
     8281 gtgctgagcg cactggttag agcacgcctg tttatgcagg acaacaatca gcaaggcaaa
     8341 ggcgtctctt tgtcaaaagc gatcaacatc attgagaacg gactgcgggt gagtcttgat
     8401 gaagagagca aagacgaatt aacccaaaac ttgattgctc tttatagcta tatggtcagg
     8461 cgcttgctgc aagccaattt acgcaacgat gtctccgcag tcgaagaagt ggaagcatta
     8521 atgcgcaata ttgccgatgc ctggaaagag tcgttactct ccccttcttt gattcaggac
     8581 ccagtctgat gaaccacgca ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa
     8641 gccagctcat gttacgcctg gcaacagaag aacaatggga cgaactcatc gccagcgaga
     8701 tggcgtatgt gaatgcggtg caggagattg cacatctgac tgaagagatt gacccgtcca
     8761 ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg
     8821 taaagcagtt attacagatt cggatggatg aactggcgaa actggtcggt cagtcatcgg
     8881 tgcaaaaatc ggtgttaagt gcctatggtg atcagggcgg ctttgtgctg gctccgcagg
     8941 ataacctctt ttgaatctga acgagtcgat ggctcgcgaa taatccgatt acggctacgc
     9001 ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt
     9061 tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc gcgccgacgg
     9121 ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag gcgcatcggg
     9181 cgggtattcg gtcggctacg actcctatga tttatttgat ttaggcgagt ttgatcagaa
     9241 aggcagcatc cctactaaat atggcgataa agcacaactg ctggccgcca ttgacgccct
     9301 gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga
     9361 tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga
     9421 agaaatcatt gagtgtgaag gctggacgcg ttacaccttc cccgcccgtg ccgggcaata
     9481 ctcgcagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg aaaaccctga
     9541 tgaagatggc atatttaaaa ttgttaacga ctacaccggc gaaggctgga acgatcaggt
     9601 tgatgatgaa ttaggtaatt tcgattatct gatgggcgag aatatcgatt ttcgcaatca
     9661 tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa cgcaatgcga
     9721 cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg ttttataaag agtggatcga
     9781 acacgtacag gaagttgcgc caaagccgct gtttattgtg gcagagtact ggtcgcatga
     9841 agtcgataaa ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc tgttcgatgc
     9901 gccgctgcag atgaaattcc atgaagcatc gcgcatgggg cgcgactacg acatgacgca
     9961 gattttcacg ggtacattag tggaagccga tcctttccac gccgtgacgc tcgttgccaa
    10021 tcacgacacc caaccattgc aagccctcga agcaccggtc gaaccgtggt ttaaaccgct
    10081 ggcgtatgct ctaattttgt tgcgggaaaa tggcgttcct tcggtgttct atccggacct
    10141 ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa acctatccga tagatatgcc
    10201 aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc acggtgtaca
    10261 gacgttattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca ctgacgaata
    10321 tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg
    10381 agagaattac ggcaataaaa cctggcgtga ttttctgggg aatcggcaag agagcgtagt
    10441 gaccgacgaa aacggcgaag cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt
    10501 tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc ggggagattt cattacggca
    10561 gtggcgtcgg taacggcgct ttatccagcg cggcggcaca ctctgccgtc gggcgatcca
    10621 cacgctccat cgtcatacca tcgtactcaa tggtgttacc gtcacgctca acctcgtaca
    10681 gctcacgttt tacggtaacg ttagtgagat catcagacat caatgtcaac ttgcccggca
    10741 cggcaatcac ccgctgccac tgacggcaat ccagggtatc gccctcttta gtgacaatca
    10801 ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc
    10861 ccgctagcca gtccggcgcg ggggttttca cgacattgtt atagttttcg acttcggcac
    10921 agcccgccag caacatgagc gcgcttgcaa ttgcgagttt tttcatcatt cgtcctgcat
    10981 gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg ccagcctttc caggttcaga
    11041 gagaaatttg atctaactat tatttttgtg aattattacc ccgaaaataa atttgcatga
    11101 tgtatgattc gcgattcttg tttcaaacct tctgagttca gaggctacat tcatgtcatg
    11161 gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg cggtcatcgc
    11221 ggcgggtatt ctctccactt actattttgg cattactggc accttttggg ctgtcacggg
    11281 tgaatttacc cgttggggcg gccagctcct gcagctgttt ggcgtccatg ctgaagagtg
    11341 gggttatttt aaaattatcc atctggaagg atcgccatta acccgcatcg acgggatgat
    11401 gatcctcggc atgtttggcg gctgctttgc cgcagcgctg tgggccaaca atgtcaaact
    11461 gcgcatgcca cgcagccgta tccgcattat gcaggccatt attggcggta ttatcgctgg
    11521 ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg gtattcctca
    11581 gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat ggtttggcgc
    11641 acgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc
    11701 ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt
    11761 ctttttcggt atgctgggct gggcgctgct cacggcgatg aaccaaccaa aactggggct
    11821 ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa tctgctttac
    11881 ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag caatcattat
    11941 cggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg ttgaacccaa
    12001 aatcatgtgg gcgggaccaa acgcggtaat tggtggttta ctgtttggtt ttggcatcgt
    12061 gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta
    12121 ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt ctggcgtatt actgggatga
    12181 tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa cctttggccc
    12241 gatgggtggc ctgctggtga catatttgct gttgtttact gcgctgatgt tgattatcgg
    12301 ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact gctaaggaga tcgcatgaaa
    12361 aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta tccggcagtc
    12421 gcaactcttg aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac
    12481 tgtccacagt cgatcaataa tattccactg gatgcgcgta atcacggcta tacggtgctg
    12541 gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc cctcattccc
    12601 gctgtggccc acagcgggaa catcgttgcc tctgacacat agattttacc cgccccctct
    12661 cctattcttt tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg cccaatccca
    12721 aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctacga
    12781 tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga
    12841 acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga
    12901 taaaccgccg ctgattaacg cccgcgtaga aactgcggcc accagtcgta tgtttaaacc
    12961 gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga
    13021 aggcgacaaa aaacagcctt attttatcta tcgcgctgat ggacaacctg tttttatggc
    13081 cgcgataggt agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac
    13141 tgcagcagca gaccagggtc tggtagatat tcatgaccgc cgcccgctgg ttctgtcgcc
    13201 agaagctgca caggaatgga tgcggcaaga gattggcggt aaagaagcat cagaaattgc
    13261 taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc gcgcggtggg
    13321 taatatcaaa aaccagggag cggagttaat tcaaccggtt tgataagcca ggagactatt
    13381 gatcaataat actgcgtcat aagaaatctc tattagacaa tagtagacgc tccttccaca
    13441 tgcgccacaa gaaacctcta ttccagtgac acaattacgc ctgattattt ccctagcccg
    13501 gaatatgaat aaaaacatca acaaagatta aattattcaa acgttactgt tgcgtctgcg
    13561 gtcgtagcaa catccgacgt ggtgatgagt gttaaaagag acactctgat gttcagaggg
    13621 gttatcgcgt ttacggatcg tggatggcag aaaactttca ggatcaggta tccattttaa
    13681 accagaaatt gagtgagttt gccccatcca tgccccaagc ggtgagattg gaagtaatta
    13741 acaacaggtt acaaaaccta cacctgcatg gacatcactt cctgatacgc cgccaccagc
    13801 ttattacgca cctgaatccc catttgcata gaaactgagg ctttttgcat atcggtcatc
    13861 acatcgttta acgccacgcc gggttcaccg agggtgaatt tttctgcctg cgtgcgggca
    13921 gctgtttgtg tatcgctaat gcgatcgagc gtggcgtgca gctgcccggc aaaactaatg
    13981 gtcggttgcg gcagtgaatc ctgcgcacgc gcactcatcg ccgtagcctg taactggctg
    14041 ataacccctt caatcccctg tatcgctgac attttcgtct cctggataat ttctggtagc
    14101 aaagcctacc agtaagtcaa taagataaag gcgctaaata acaacaaaaa aacgggttta
    14161 ttggcggata gaaaaaaacg aaagcacaaa taatggcagc gtcaattttt cgagtttgct
    14221 gacccgggag tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg
    14281 tgcgcgatga atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt
    14341 gcgaatccga aaattccatt gattgttgcc ggttccgcgg cagtggcggt catggtcgca
    14401 ctgatcctgt gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag
    14461 gatggtggcg caattgtcag ccaactgacg caaatgaata ttccttaccg cttcagcgaa
    14521 gccagcggcg ctattgaagt tccggcagat aaagttcacg aactgcgtct gcgcctggca
    14581 caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac tactcgatca ggaaaagttt
    14641 ggtatcagcc agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct
    14701 cgtaccatcg aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa
    14761 ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta
    14821 cccggccgcg cactcgatga agggcaaatt agcgccattg tgcatctggt ttccagcgcc
    14881 gttgctggtc tgccgccggg aaacgtcacg ctggtggatc agggcggaca tctgttaacc
    14941 cagtccaata ccagcgggcg cgatcttaat gatgctcagt tgaaatatgc cagcgatgtc
    15001 gaaggccgta ttcagcggcg tattgaagca atcctgtcgc ctattgttgg taacggtaat
    15061 attcacgccc aggtcacggc gcagctggac ttcgccagta aagaacaaac ggaagaacag
    15121 tatcgcccta acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc
    15181 gagcaaagcg gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca
    15241 cctgcgaata acgcgccaat cagcacgcct ccggcaaatc aaaataaccg ccagcagcag
    15301 gcgagcacca ccagcaatag cgggccgcgt agcacacagc ggaatgaaac cagtaactac
    15361 gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca
    15421 gtcgcggtag tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac
    15481 gaacagatga agcaaattga agatctgacc cgcgaggcga tgggcttttc tgaaaaacgc
    15541 ggtgactcgc tcaatgtcgt taactcgccg ttcaatagca gtgacgaaag cggcggagaa
    15601 ctgccgttct ggcaacagca agcgtttatc gatcagttgc tggctgccgg tcgctggttg
    15661 ctggtactgc tggtggcgtg gctgctgtgg cggaaagcgg tacgcccgca gttaacacgt
    15721 cgcgccgagg cgatgaaagc tgtacagcaa caggcgcagg cccgtgagga agtggaagat
    15781 gcggtggaag tccgcctgag caaagacgaa caactccaac aacgacgcgc taaccaacgt
    15841 ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga tccgcgcgtg
    15901 gtggcgctgg tcattcgcca gtggataaat aacgatcatg agtaacctga caggcaccga
    15961 taaaagcgtc atcctgctga tgaccattgg cgaagaccgg gcggcagagg tgttcaagca
    16021 cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg gcgaacgtca cgcagatctc
    16081 caacaagcag ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac agtttgccgc
    16141 actgaatatc aacgccaacg attatctgcg ttcggtattg gtcaaagctc tgggtgagga
    16201 acgtgccgcc agcctgctgg aagatattct cgaaactcgc gataccgcca gcggtattga
    16261 aacgctcaac tttatggagc cgcagagtgc cgccgatctg attcgcgatg agcatccgca
    16321 aattatcgcc accattctgg tgcatctgaa gcgcgcccag gccgccgata ttctggcgtt
    16381 gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg gcggcgtgca
    16441 gccagccgcg ctggcggaac tgaccgaagt actgaatggc ttgctcgacg gtcagaatct
    16501 caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc tgatgaaaac
    16561 tcagcaggaa gaagccgtta ttaccgccgt gcgcgaattc gacggcgagc tggcgcaaaa
    16621 aattatcgac gagatgttcc tgttcgaaaa tctggtggat gtcgacgatc gcagcattca
    16681 gcgtctgttg caggaagtgg attccgaatc gctgttgatc gcactgaaag gggccgagca
    16741 gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata ttctgcgcga
    16801 cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac agaaagcgat
    16861 tctgctgatt gtgcgccgcc ttgccgaaac tggcgagatg gtaattggca gcggcgagga
    16921 tacctatgtc tgataatctg ccgtggaaaa cctggacgcc ggacgatctc gcgccaccac
    16981 aggcagagtt tgtgcccatg gtcgagccgg aagaaaccat cattgaagag gccgaaccca
    17041 gccttgagca gcaactggcg caactgcaaa tgcaggccca tgagcaaggt tatcaggcgg
    17101 gtattgccga aggtcgccag caaggtcata agcagggcta tcaggaagga ctggcccagg
    17161 ggctggagca aggtctggca gaggcgaagt ctcaacaagc gccaattcat gcccggatgc
    17221 agcaactggt cagcgaattt caaactaccc ttgatgcact tgatagtgtg attgcttcgc
    17281 gcctgatgca gatggcgctg gaggcggcac gtcaggtcat cggtcagacg ccaacagtgg
    17341 ataactcggc actgatcaaa cagatccaac agttgttgca gcaagaaccg ttattcagcg
    17401 gcaaaccgca actacgcgtg caccccgatg atctgcaacg tgtggatgat atgctcggcg
    17461 caaccttaag tttgcatggc tggcgcttgc ggggcgatcc cacactccat cctggcggct
    17521 gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc tggcaagaac
    17581 tctgccgtct ggcagcacca ggagtggtgt aatgaccacg cgcctgactc gctggctaac
    17641 cacgctggat aactttgaag ccaaaatggc gcagttgcct gcggtacgtc gctacgggcg
    17701 attaactcgc gctaccgggc tggtgctgga agccaccgga ttacaattgc cgctcggcgc
    17761 aacctgtgtc attgagcgcc agaacggcag cgaaacgcac gaagtagaaa gcgaagtcgt
    17821 tggctttaac ggtcaacggc tgtttttaat gccgctggag gaagtcgaag gtgtcctgcc
    17881 cggcgcgcgt gtttatgcca aaaacatttc ggcagaaggg ctgcaaagcg gcaagcagtt
    17941 gccgctcggt ccggcgttat taggtcgtgt tctggacggc agcggtaaac cgctcgatgg
    18001 cctcccctcc cccgatacga cggaaaccgg tgcgctgatt accccaccat ttaacccgtt
    18061 gcaacgtaca ccgattgaac atgtgctgga caccggcgtg cgcccaatca atgccctgct
    18121 taccgttggg cgtgggcagc gtatggggct gtttgccggg tccggcgttg gtaaaagtgt
    18181 gctgctgggg atgatggcac gttacacccg cgccgatgtc attgtcgtgg gtttgattgg
    18241 tgaacgcgga cgcgaagtaa aagattttat tgagaatatc ctcggtgccg aagggcgtgc
    18301 acgctccgtg gtgattgccg ctccggcgga tgtctctccg cttctgcgaa tgcaaggtgc
    18361 cgcctatgcc acgcgaattg ccgaagattt tcgcgatcgt ggtcagcatg tgttgctgat
    18421 tatggactcc ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg cgattggcga
    18481 accacctgcc actaaaggtt atccaccgtc ggtgtttgcc aaattaccgg cactggtcga
    18541 gcgtgccgga aatggaatta gcggcggcgg ctcgattacc gcgttttata ccgtgctcac
    18601 cgaaggcgat gaccagcagg accccattgc cgactctgcg cgggcaatcc tcgacggtca
    18661 cattgtgctg tctcgccgac tggcggaagc cgggcactat ccggctatcg atattgaagc
    18721 gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc gagtgcgcac
    18781 cttcaaacag ctgttgtcga gttttcagcg taaccgcgat ctggttagcg tcggcgcgta
    18841 tgccaaaggc agcgatccga tgctcgacaa agccatcgcc ctgtggccgc agctggaggg
    18901 ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa gcgtctctcc aggggctgga
    18961 gcgtattttc ccgacagtgt cataacccag gagataacgg cagatggcag aacatggcgc
    19021 gctggcgacc ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc gcctgctggg
    19081 tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc aaaatgctga tcgattatca
    19141 gaatgaatat cgcaataacc tcaacagcga tatgagtgcc gggatgacca gcaaccgctg
    19201 gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca
    19261 acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca
    19321 acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga
    19381 aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc
    19441 tgaatgattc gcttagcgcc cttaattacc gccgacgttg ataacgccac attgcctggc
    19501 ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc attagcaggc
    19561 gagacaacta ccgacaaagc ggccccccag ttgctggtgg caacagataa gcccacgaca
    19621 aaaggcgagc cgctggtcag cgagattctt gccgatgcac aacaagcgga tttactgatc
    19681 cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat ccacatcaac accattaacc
    19741 accgctcaaa cgatgacgtt ggctgcggtg gctgacaaca atacggcaaa agacgaaaaa
    19801 gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg ccctttttgc gatgttgccg
    19861 ggttttgaca atacgcccaa agtgactgat gtgccgtcaa ccgtgttacc ggcagagaaa
    19921 ccaacgctct tcacaaaact gacttctgcg caactcacaa cagcacagcc tgatgatgcc
    19981 cccggcacgc cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa
    20041 gtcatcagca caccttcacc ggtgaccgct gccgccagcc cgctaatcac tccacaccag
    20101 acacagccac tgcccaccgt cgccgtgcct gtgttgagtg caccgctggg ttctcacgaa
    20161 tggcaacaat cattaagcca gcatatttcg ctgttcaccc gccagaggca acaaagtgca
    20221 gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa tctccctcaa agtggatgat
    20281 aaccaggcac aaatccagat ggtttcaccg catcagcatg tacgcgccgc gctggaagca
    20341 gcgcttccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg gcaaagcaac
    20401 atcagtggcg aaagctttag tggtcagcag caggccgctt cccaacaaca gcaaagccaa
    20461 cgcacagtaa accatgaacc tctggcgggg gaagacgacg atacgcttct ggttcccgtc
    20521 tctatacaag ggcgcgtaac aggcaacagc ggcgttgata ttttcgccta acgtcagagg
    20581 tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc
    20641 cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga
    20701 aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg gcctgtgcca
    20761 gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac
    20821 gcgtcgtacc ctccccggtc ttctacgcgc tggatacctt cacggtcaat ctgggcgatg
    20881 cggatcgcgt gctttatatc ggcataaccc tgcgcctgaa agatgaagct acccgctcgc
    20941 ggctgagtga gtatttgccg gaagtccgta gccgtttgct gttactgttt tcgcgtcagg
    21001 atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca
    21061 cgctttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgctgtata
    21121 ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat
    21181 gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa
    21241 agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag
    21301 gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg
    21361 ctgcgtcgta gcccggatat aaccgtcggg gccatccgca ttcagccgta ccatgaattt
    21421 gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc atctgaaacc gctgcgcggc
    21481 acagggctgg tggtgttttc accgagtctg gtgtttatcg ccgtggataa cctgtttggc
    21541 ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac cgaacagcgc
    21601 gtcatcaacc gcatgttaaa gctggcgctt gaaggctata gcgacgcctg gaaggcgatt
    21661 aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tgaaatttac caatatcacc
    21721 acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc
    21781 ggcgaattta atatctgcct gcctttcagc atgatcgagc cgctacggga attgttggtt
    21841 aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc
    21901 caggtgcagc attcacagct ggagctggtc gccaactttg ccgatatctc gctacgcctg
    21961 tcgcagattt taaaactgaa acccggtgac gtcctgccga tagaaaaacc cgaccgcatc
    22021 atcgcccatg ttgacggcgt cccggtgctg accagtcagt acggcactct caacggtcag
    22081 tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag
    22141 cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt
    22201 gggctgaagc tttgagcgaa caaaaatcaa ccagcagcaa aagcgctgcc gacgcggtgt
    22261 tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgattatgg
    22321 atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt
    22381 tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct ggcgggcgaa ccactggata
    22441 ttctgatcaa cggttattta attgcccagg gcgaagtggt ggtcgttgcc gataaatatg
    22501 gcgtgcggat caccgatatc attactccgt ctgagcgaat gcgccgcctg agccgttagt
    22561 gatgaataac cacgctactg tgcaatcttc cgcgccggtt tctgctgcgc cactgctgca
    22621 ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa
    22681 acggttggga tttgtcccta aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc
    22741 ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg gaagatgcac ggctggtgct
    22801 cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacgga
    22861 agagataccg cagcccgatt ttcagtcggt tatgaaaaac ttgcttaagc gtagcgggag
    22921 atcctgatgc gtcgtttgtt gtctgtcgca cctgtccttc tctggctggt tacgcccctc
    22981 gccttcgcgc aactgccggg tatcaccagc cagccgctgc ctggcggtgg acaaagctgg
    23041 tcgctcccgg tgcaaacgct ggtgttcatc acctcgttga cgtttattcc ggcaatttta
    23101 ctgatgatga ccagtttcac ccgcatcatc attgtttttg gtttattgcg taacgcgttg
    23161 ggaacaccct ccgcgccacc taaccaggta ttgctgggac tggcgctgtt tttgaccttt
    23221 tttattatgt cgccggtgat cgataaaatt tatgtcgatg cgtaccagcc attcagcgaa
    23281 gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg
    23341 ctgcgtcaga cccgcgaggc ggatttaggg ttgtttgcca gactggcgaa tactggcccg
    23401 ttgcagggac ctgaagctgt gccgatgcgc attttgctcc cggcctacgt gaccagcgag
    23461 ttgaaaaccg catttcagat aggcttcacg attttcattc cttttttgat tatcgacctg
    23521 gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttcccccagc cacaattgct
    23581 ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg
    23641 ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg
    23701 gggactgaag cgatgaaagt cgcgctggca ctggctgccc cactgttgct ggtggcgctg
    23761 gtcacgggtc ttatcatcag tattttgcag gccgccaccc agattaacga aatgacgctg
    23821 tcgtttattc cgaaaatcat cgccgtattt atcgccatta ttattgccgg accgtggatg
    23881 ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg
    23941 taggccgtac aatgatgcag gtaacaagcg atcaatggct ttcctggtta agcctgtact
    24001 tctggccgtt actgcgcgtg ctggcgctca tttctaccgc accaattctg agcgaacgca
    24061 gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgct attgccccat
    24121 cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtgcagc
    24181 agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa
    24241 ccgctggtga aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt gtcgatccgg
    24301 ccagccatct caatatgccc gttttagcgc gtatcatgga tatgctggcg ttactgctgt
    24361 tcctgacatt taacggtcat ttatggttga tttcactgct ggccgatacc tttcacaccc
    24421 tgccaattgg tggcgaaccg ttgaacagca atgcgtttct ggcactcacc aaagcaggga
    24481 gtttgatttt ccttaacggg ctgatgctgg cattaccgct cattactctg ctgctgaccc
    24541 tgaatctggc attaggctta cttaatcgta tggccccgca attatccatt tttgttattg
    24601 gatttccatt aattctgact gtcggtatct ctttaatggc ggcattaatg ccgttaattg
    24661 cgcctttttg cgaacattta ttcagtgaga tgtttaattt gctggctgat attattagtg
    24721 aattgccatt aatataattc cgtaacgttt atcatgttat cctaaggatt atccgaaaaa
    24781 taatacctac gaacatcttc caggatactc ctgcggcgaa atatttgttt taagctcact
    24841 cacatatcgc aacatttact ttactttaag acaattccag gcaaattata caacacttta
    24901 cgggatagta agtccgcctg aaaaatcgcg agagtggcgc attaggtgac ccatgttgtt
    24961 ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg
    25021 tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg
    25081 tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac
    25141 cttgccatag cttgtgattc acagcgccct tcagtggtgt ttattaatga ggactgtttc
    25201 atccacgatg cttctaacag tcagcgtatc aagctcatca ttaatcaaca tcccaatacg
    25261 ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct attggtcaga
    25321 aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgacga tatccttggc
    25381 gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac gttatcattg
    25441 agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc
    25501 tctgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaaacgt
    25561 aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg
    25621 actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc
    25681 aggccgaatt agattactct gctttctcca caaagatacc gtcctgatgc cctgcttcat
    25741 taaagaacca gatgccgagc gggtagtctt ccagcgaaac caggtacatt gtgccttcac
    25801 taaactcctc aactgccagt accacgccag gacgacgcgg accgccatcc gttttgactg
    25861 ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat
    25921 ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg gtctatactt
    25981 aagagatgcc aggcggactt aacgactggc cgcaacaaca gagtaacggt tgcgaggaaa
    26041 gatgatgaaa accgcaaaag agtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt
    26101 cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa
    26161 cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga
    26221 tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa
    26281 taaaaaaatc ccgaccctga gggggtcggg atgaaacttg cttaagcaag aagcacttaa
    26341 aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatccgcaat ttttttcgct
    26401 gacaagaaaa tattcgtgat atgaatgatt aacgctactt atgaatagaa atgtgacgcg
    26461 cgtcacccct tggtggctct ggcgtcaacg ggatcgctcc cgaaaaaatc atctttgata
    26521 atttgtgctg ttttgagaat catcctggta aggggagtac tgcgcaacca tgctttcaat
    26581 tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta
    26641 tgactggcaa ccagctgccc cctggctcag ccgtttacgc gaagcgaatg ttcccgtcat
    26701 tctctgtagc agtaagacat cagccgaaat gctgtacttg caaaaaacgt tggggctaca
    26761 aggtttaccg ctgattgccg aaaatggcgc agtgatccag cttgctgagc aatggcagga
    26821 tatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatca gccaggtttt
    26881 aaatacgcta cgcgagaaag aacattttaa attcacaact tttgatgatg tcgacgatgc
    26941 aaccatcgcc gaatggacgg gattaagccg tagccaggcg gcgctgacgc agctacatga
    27001 ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttaccgctcg
    27061 tctgaacgaa ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tcctggatgc
    27121 ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac aatcgtcagg
    27181 caaacgccca accacacttg gcctgggcga tgggccaaac gatgcgccct tactggaggt
    27241 aatggattac gcggtgattg tgaaagggtt aaaccgtgaa ggggtgcatc tgcatgatga
    27301 ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct
    27361 ggaccatttt ttctccgccc attaagcgtt atcgctcgcg aatacccgat tgcgcccggc
    27421 ctgtttagcg agataaagtc gacggtcggc cagtgactgg agttgttcaa aatcataatc
    27481 accggtttcc tcgctgctgc ttacccccag cgaggcactg atgcgtatcg tcgtactctt
    27541 ggcgatcaac atctcttttt catttagctt caggcgaata cgttctgcaa cctccgcagc
    27601 ctgcgtcagg ctcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc
    27661 aacatcctgc gctcgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc
    27721 gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaaggt cgacctgaat
    27781 gacagaaaaa ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa
    27841 cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc agtgcaacga
    27901 gctttgcaaa acatacatgt tgctgaccat ccggcgaatc acataccagg agatgagtaa
    27961 catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc
    28021 gcgcacgcct tcgcttagcg tatggacgcg caccagcaca ccgtcgaaat ggtccagacg
    28081 ttcccagcta acatagcgac tgttcatacg aatgccgccc cgcgtgtcat gttccattgc
    28141 ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt
    28201 ggaagaggtc aataatctca gcttactgtc atagagctga tactcaccat cgaggttttt
    28261 atcgatggcg tttcttaaaa attgctgcat ggtacgcacg ggaatactca tccccagcac
    28321 gccataccag tagttttcac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt
    28381 gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg
    28441 cgaatggccg acaaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg
    28501 cgtaaacaag gtcggctgcg tcgaaacgta aaatccggca cgcgagacat acatcgcctg
    28561 ttcaaccatc gacgaggagt tgtgtgccag tcgcagcaag taaccaactt ccagtgcagc
    28621 ggtaatttca ttgtcgaggc tttcattttc gcgagacagg agattcccct cgctgactaa
    28681 tgcatccgac acaccattga ctggcagggt gcgtcgtcgg ttgagttcga tttgccaggc
    28741 gtgctcgtcg cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag
    28801 tggcgctacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata
    28861 ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct ggctggagat
    28921 ataggcatct tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac
    28981 cacgataaag cagagattaa cgacatgacc aggccctagg cggcgtgcga gttttttcaa
    29041 ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt
    29101 ttatgattct ggcataacct ggcgatagcg taccgcaaaa cagcgataat tttataatgg
    29161 tggcatgata agacgcgaca gacatcgata gttttataac gtaggcatga taagacgcga
    29221 tagcgtcgca tcaggcaatt cagtgcagac actggcacac ccgaccggat gcgacgtgag
    29281 cgccacatcc ggtcgggtgt taatgatacc ctttcaaact tcttccgcag aacgactccc
    29341 ctcccccgga acaagctcgg tttcatgaaa agcttcccgc ttgaccccgt aaccgtcata
    29401 ccaacgacac tcaaccatac cgctggagta tccggtgaca atcatccgcg ggccgccctc
    29461 tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt ttcgagtgaa
    29521 acttgttcac cttagttgaa gatggcgaat tttgcataat agcctgtgcg agtattaatg
    29581 tgcctgaccg cgtattttcg ctacggcttt cactcccagc accacgatgc cgccgatgat
    29641 aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg cactttgctg
    29701 cccggcgaaa tgttcaatgg cgtgatgcag cggcgcaatt ccatgtacca caatcccacc
    29761 gccgacgagg aacatcgcca gcgtgccgac aatcgataac gccttcatca accagggcgc
    29821 aatgatcaat aaccctttgc ctaatgcctg catcagcgcg ctggattttt ccgccagcca
    29881 ataccccagg tcatcaatct taacgataac ccctaccaga ccgtagacgc ccacggtcac
    29941 taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgctt cggccacaat
    30001 ccccagcgtg atggcgacga tttccgcaga caatataaaa tcggtacgaa tcgccccttt
    30061 tattttgtct ttttcaaact tcagcggatc ctgcgccgcc agcttctcca gacgctgctg
    30121 actctgcgcc ggatcttctt tatgtttacg cgcctccagc atatgcagca ctttctctac
    30181 cccttcaaag cagagaaacg cgccgccaat catcaacagt ggcgtaatcg cccacgggat
    30241 aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca gcgatccttt
    30301 cgccacgccc cagaccacgg gaagttcccg gttggcgcgc acgcctgcaa cttgttgcgc
    30361 attgagcgat aagtcatccc ctaatacacc ggcggttttc ttcgccgcca gtttgcccat
    30421 cacggagata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta aactactccc
    30481 ggccaaaagc actccctctc atttttattg ttgaagggtg taagtatgaa gcaaaaacgg
    30541 cagctctgat acttgcttca caggtcaaca tttatttaac aatcaagtgc aattatatct
    30601 ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc agccgtgagg
    30661 gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt tgtatatcat
    30721 ttggggctca acctattttg tcattcggat tggcgtggaa agctggcctc cgttaatgat
    30781 ggcgggcgtt cgattcctgg cagccggtat tttattgctg gcatttttgc tactgcgcgg
    30841 acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc tgttattgct
    30901 ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt ccggcatcgc
    30961 cgccgttgtg gttgcaaccg tgcccctctt taccctgtgc ttcagccgcc tgtttggcat
    31021 taaaacgcgc aaactggaat gggtgggtat cgccattggg cttgccggaa tcatcatgct
    31081 caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt taatcggctc
    31141 gattagctgg gcgtttggct cggtttatgg ctcgcgcatt accttacctg tagggatgat
    31201 ggcgggtgcg attgagatgc tggcggctgg cgtggtgtta atgattgcgt cgatgattgc
    31261 gggtgaaaaa ctgacggcgc tcccttccct ttcaggcttc cttgcggtcg gctatctggc
    31321 gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgca acgtcagtcc
    31381 ggctctcgcc accagctacg cctacgttaa cccagtggtc gcggtcttgc tgggtacggg
    31441 gctgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa ttgttttcgc
    31501 ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aaacccgttg tcgcgccagt
    31561 tattccggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc gcgctggcac
    31621 cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgtc agcttctcgc
    31681 gcccgcgtaa cgcacattcc cagacaatca atacgcgcca accgagttcc tgcaaatggc
    31741 tgatatcacg acgatcgcgc tcgacatttt tacctatctt ctccagccaa aaatctgttc
    31801 gcgtggcggg gactttaaac aggtagcaat ggtgatggtg ccagaagcaa ccgtgggcaa
    31861 atatcacaca gcgaaattca tcaaggacaa aatccgggcg tccgggcaga ctggcgtcct
    31921 gaacgcgaaa cgccaggccc tgcccagtta acaaactggc gaggcgcttc tcaatcgccg
    31981 tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg tgaacgtcga
    32041 ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg ctcaagcagt
    32101 tttgccaccg cggcaaagac cggcacgacc accgagttac cgaactggcg ataagcctga
    32161 gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc cattaagcgc
    32221 gcacattccc gaggcgttaa ccgacgtggg cgatgttgct gattcagcgg atcgtcaaag
    32281 tctttctcac ccgttgccat atcccagccg cgatcgatta aaatttccgc gccatctttg
    32341 taataacgcg cagacagcgt acgcgtgacg ctttgcggat tgttcggata aaccattcca
    32401 taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat atcgatagag gtacttccac
    32461 agtaccggcg tcaggatata tttcgcctcg accatcgggt ccagcagctg cgccagcgtc
    32521 actcgctgtg cagggaaaca tttgctgata tcacgcagag taaaatcggc tttcagattc
    32581 agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag aaaatgtttg
    32641 ccgtcgatga ttttcggatc gtccggcccg ttatcttctg catcagccac gtcatagccc
    32701 agttcgtcca gcgtctgcat gatgatgcgg aacgttttac cctggtcgtg acttttcagg
    32761 tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat gcgcaccaca
    32821 tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc gagcgagttc
    32881 tttttcgata cgccagccag cgaaaatggc tgacaaggga aaccggccag taaaacatcg
    32941 tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac gccttcttga
    33001 tggctgaggg tgatgtcgcg gatatcctca ttaaaatgat gcgtcgccgg gtcgcaataa
    33061 tggttggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa cacgcactgt
    33121 ccaccaatcg attcaaaacc gcgacggatg ccgccaattc cggcaaatag atcgataaag
    33181 cgaaacgcat aatgcggatg atgtgccggt ggtttgggta acaacgtttg cagatgggcg
    33241 aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg ttttaaaatt
    33301 gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcgt tttaacatca
    33361 taaatttgca gcagtttctc cagcattgcc tgtgcggcat tcccggtgct gtatgaatcg
    33421 gttactgata tattttcctg catagattta accggccatc tgagatggcc gacaggttaa
    33481 cataattact gccgtttagg gccacgcgct cagccagcag gtgaaaacgc atctatcacc
    33541 ttgtgatcga taccttcatt ctcccccgcc agttcggcac cgagcttcgc cataaactcg
    33601 actaaaaatt gcgcgttgtg ctgtgccagc tgcttgcctc ttgcggtttg catggtttgc
    33661 ggtaatttca gtaactttgt ctggaaatga tccagcgcgt agcgtttatc atcaagcggg
    33721 cgatgctgtg cgaacggatc ttcgccatca aacagcgcca agcccaatgc ccctgaaacg
    33781 gcaaatacgc gcgccaggcc aatcgccccc agggcttcca gccgatccgc atcctggacg
    33841 attttagctt ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc ggcaatggca
    33901 tgacaaacgg cctcaatttt ctccgccgga aattgcacga actctt
//
LOCUS       DAZFKM010000038        33827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFKM010000038 DAZFKM010000000
VERSION     DAZFKM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..1231
                     /gene="citC"
                     /locus_tag="ACHKAY_001967"
     CDS             173..1231
                     /gene="citC"
                     /locus_tag="ACHKAY_001967"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO5700648.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1246..1542
                     /gene="citD"
                     /locus_tag="ACHKAY_001968"
     CDS             1246..1542
                     /gene="citD"
                     /locus_tag="ACHKAY_001968"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO5700649.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1539..2447
                     /gene="citE"
                     /locus_tag="ACHKAY_001969"
     CDS             1539..2447
                     /gene="citE"
                     /locus_tag="ACHKAY_001969"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO5700650.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2458..3990
                     /gene="citF"
                     /locus_tag="ACHKAY_001970"
     CDS             2458..3990
                     /gene="citF"
                     /locus_tag="ACHKAY_001970"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO5700651.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3994..4545
                     /gene="citX"
                     /locus_tag="ACHKAY_001971"
     CDS             3994..4545
                     /gene="citX"
                     /locus_tag="ACHKAY_001971"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO5700652.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4520..5398
                     /gene="citG"
                     /locus_tag="ACHKAY_001972"
     CDS             4520..5398
                     /gene="citG"
                     /locus_tag="ACHKAY_001972"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO5700653.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5449..6912
                     /gene="citT"
                     /locus_tag="ACHKAY_001973"
     CDS             5449..6912
                     /gene="citT"
                     /locus_tag="ACHKAY_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO5700654.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7026..7832
                     /gene="rna"
                     /locus_tag="ACHKAY_001974"
     CDS             7026..7832
                     /gene="rna"
                     /locus_tag="ACHKAY_001974"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO5700655.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8062..8472
                     /gene="rnk"
                     /locus_tag="ACHKAY_001975"
     CDS             8062..8472
                     /gene="rnk"
                     /locus_tag="ACHKAY_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO5700656.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8610..8699
                     /gene="yldA"
                     /locus_tag="ACHKAY_001976"
     CDS             8610..8699
                     /gene="yldA"
                     /locus_tag="ACHKAY_001976"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO5700657.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8703..9941)
                     /gene="ybdR"
                     /locus_tag="ACHKAY_001977"
     CDS             complement(8703..9941)
                     /gene="ybdR"
                     /locus_tag="ACHKAY_001977"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO5700658.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10162..10590
                     /gene="uspG"
                     /locus_tag="ACHKAY_001978"
     CDS             10162..10590
                     /gene="uspG"
                     /locus_tag="ACHKAY_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO5700659.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10711..12276)
                     /gene="ahpF"
                     /locus_tag="ACHKAY_001979"
     CDS             complement(10711..12276)
                     /gene="ahpF"
                     /locus_tag="ACHKAY_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO5700660.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12447..13010)
                     /gene="ahpC"
                     /locus_tag="ACHKAY_001980"
     CDS             complement(12447..13010)
                     /gene="ahpC"
                     /locus_tag="ACHKAY_001980"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO5700661.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13382..14128
                     /gene="dsbG"
                     /locus_tag="ACHKAY_001981"
     CDS             13382..14128
                     /gene="dsbG"
                     /locus_tag="ACHKAY_001981"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO5700662.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14337..15239
                     /gene="ybdO"
                     /locus_tag="ACHKAY_001982"
     CDS             14337..15239
                     /gene="ybdO"
                     /locus_tag="ACHKAY_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5700663.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15386..16606
                     /gene="ybdN"
                     /locus_tag="ACHKAY_001983"
     CDS             15386..16606
                     /gene="ybdN"
                     /locus_tag="ACHKAY_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5700664.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVTLPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16579..17208
                     /gene="ybdM"
                     /locus_tag="ACHKAY_001984"
     CDS             16579..17208
                     /gene="ybdM"
                     /locus_tag="ACHKAY_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO5700665.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17209..18369)
                     /gene="ybdL"
                     /locus_tag="ACHKAY_001985"
     CDS             complement(17209..18369)
                     /gene="ybdL"
                     /locus_tag="ACHKAY_001985"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO5700666.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18478..19566
                     /gene="hcxA"
                     /locus_tag="ACHKAY_001986"
     CDS             18478..19566
                     /gene="hcxA"
                     /locus_tag="ACHKAY_001986"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO5700667.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19576..19773)
                     /gene="ybdD"
                     /locus_tag="ACHKAY_001987"
     CDS             complement(19576..19773)
                     /gene="ybdD"
                     /locus_tag="ACHKAY_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5700668.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(19955..22060)
                     /gene="cstA"
                     /locus_tag="ACHKAY_001988"
     CDS             complement(19955..22060)
                     /gene="cstA"
                     /locus_tag="ACHKAY_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO5700669.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22241..22654)
                     /gene="entH"
                     /locus_tag="ACHKAY_001989"
     CDS             complement(22241..22654)
                     /gene="entH"
                     /locus_tag="ACHKAY_001989"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO5700670.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22657..23403)
                     /gene="entA"
                     /locus_tag="ACHKAY_001990"
     CDS             complement(22657..23403)
                     /gene="entA"
                     /locus_tag="ACHKAY_001990"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO5700671.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23403..24260)
                     /gene="entB"
                     /locus_tag="ACHKAY_001991"
     CDS             complement(23403..24260)
                     /gene="entB"
                     /locus_tag="ACHKAY_001991"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO5700672.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24274..25884)
                     /gene="entE"
                     /locus_tag="ACHKAY_001992"
     CDS             complement(24274..25884)
                     /gene="entE"
                     /locus_tag="ACHKAY_001992"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO5700673.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25894..27069)
                     /gene="entC"
                     /locus_tag="ACHKAY_001993"
     CDS             complement(25894..27069)
                     /gene="entC"
                     /locus_tag="ACHKAY_001993"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO5700674.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27438..28394
                     /gene="fepB"
                     /locus_tag="ACHKAY_001994"
     CDS             27438..28394
                     /gene="fepB"
                     /locus_tag="ACHKAY_001994"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5700675.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(28398..29648)
                     /gene="entS"
                     /locus_tag="ACHKAY_001995"
     CDS             complement(28398..29648)
                     /gene="entS"
                     /locus_tag="ACHKAY_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO5700676.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            29759..30763
                     /gene="fepD"
                     /locus_tag="ACHKAY_001996"
     CDS             29759..30763
                     /gene="fepD"
                     /locus_tag="ACHKAY_001996"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO5700677.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30760..31752
                     /gene="fepG"
                     /locus_tag="ACHKAY_001997"
     CDS             30760..31752
                     /gene="fepG"
                     /locus_tag="ACHKAY_001997"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO5700678.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31749..32564
                     /gene="fepC"
                     /locus_tag="ACHKAY_001998"
     CDS             31749..32564
                     /gene="fepC"
                     /locus_tag="ACHKAY_001998"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5700679.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32561..33694)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKAY_001999"
     CDS             complement(32561..33694)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKAY_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO5700680.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         8100 a         9225 c         8545 g         7957 t
ORIGIN      
        1 aatcaaaatt tcacgccgaa atactcctta ggatgtatag cgaaaggaga aaaagatata
       61 cctcgatcac ccccctttct cccaagtgaa aataaaaggt tatcagtttg caacattgaa
      121 caacattcgt tgcaaatcga taacaacatg caccttcagg atactattta ttatgttcgg
      181 caatgatatt ttcacccgcg taaaacgttc agaaaataaa aaaatggcgg aaatcgccca
      241 attcctgcat gaaaatgatt tgagcgttga caccacagtc gaagtattta ttaccgtaac
      301 ccgcgatgaa aagcttatcg cgtgcggtgg aattgccgga aatattatta aatgcgttgc
      361 tatcagtgaa tccgttcgcg gtgaaggact ggcgctgaca ttagccactg aactgataaa
      421 cctcgcctat gagcggcaca gcacgcatct gtttatttat accaaaaccg aatacgaggc
      481 gctgttccgc cagtgcggtt tttccacgct gaccagcgta cccggcgtga tggtgctgat
      541 ggaaaacagc gccacgcggc tgaaacgcta tgccgaatcg ctgaaaaaat ttcgtcatcc
      601 agggaacaag attggctgca ttgtgatgaa cgccaatccc ttcacgaatg gtcaccgtta
      661 tctgattcaa caagctgcgg cacagtgcga ctggttgcat ctgtttttag tcaaagaaga
      721 ttcttcacgc ttcccctatg aagaccggtt ggatctggtg ttaaaaggca ccgccgatat
      781 tccacgcctg actgtgcatc gcggctccga atacatcatc tcccgcgcta cgttcccttg
      841 ctacttcatt aaagaacaga gcgtcattaa ccattgttac accgaaattg atctgaagat
      901 tttccgtcag tacctcgctc ccgcgctggg tgtaactcac cgctttgtcg gtactgaacc
      961 cttttgtcgc gttaccgccc agtacaacca ggatatgcgc tactggctgg aaacgccgac
     1021 tatctccgca ccgcccatcg aactggttga aattgagcgg ctgcgttacc aggagatgcc
     1081 gatatccgct tcccgggtac gtcaactgct ggcgaaaaac gatctcacgg ctatcgcgcc
     1141 gctggtccct gcagtcacgc tgcattattt gcagaacctg cttgagcact cccgccagga
     1201 cgcggcagct cgtcaaaaga cccccgcatg agaaacaggt gaaaaatgaa aataaaccag
     1261 cccgccgttg caggcaccct tgagtctggg gatgtgatga tacgcatcgc cccactcgat
     1321 acgcaggata tcgacctgca aatcaatagc agcgttgaga aacagtttgg cgatgcaatt
     1381 cgcaccacca ttctggacgt tctcgcccgc tacaacgtgc gcggcgtaca gctgaatgtc
     1441 gatgacaaag gcgcactgga ctgcatttta cgtgcacgac tggaagccct gctggcacgc
     1501 gccagcggta tcccggctct gccatgggag gattgccaat gatttccgct tcgctgcaac
     1561 aacgtaaaac tcgcacccgc cgcagcatgt tgtttgtgcc tggtgccaat gccgcgatgg
     1621 tcagcaactc cttcatctac ccggctgatg ccctgatgtt tgacctcgaa gactccgtgg
     1681 cattacgtga aaaagacacc gcccgccgca tggtttatca cgcgctgcaa catccgctgt
     1741 atcgcgatat tgaaaccatt gtgcgtgtca acgcgctgga ttccgaatgg ggtgttaacg
     1801 acctggaagc cgtcgttcgc ggtggtgcgg acgttgtgcg tctgccgaaa accgataccg
     1861 ctcaggatgt tctggatatt gaaaaagaga tcctgcgtat cgaaaaagcc tgtggtcgtg
     1921 aacccggcag caccggcctg ctggcggcga ttgaatctcc gctgggcatt acccgcgcag
     1981 tggaaatcgc tcacgcttcc gagcgtttga tcggtatcgc cctcggtgca gaagactatg
     2041 tgcgcaacct gcgtacagaa cgctccccgg aaggaactga actgctgttc gcacgctgtt
     2101 ccattttgca ggccgcgcgc tctgcgggta ttcaggcgtt cgataccgtc tattccgacg
     2161 ctaacaacga agccggattc ctgcaagaag ccgcccacat caaacagctg ggctttgacg
     2221 gcaaatcgct gatcaacccg cgtcagattg atctgttgca caacctctac gcaccaaccc
     2281 agaaagaagt ggatcacgcc cgccgcgtcg tagaagccgc tgaagccgcc gctcgcgaag
     2341 gcctcggcgt ggtttccctg aacggcaaga tggtggacgg tccggttatc gatcgcgccc
     2401 gtctggtgct ctctcgtgca gaactttccg gcatccgcga agaataaggc aatcaaaatg
     2461 acgcagaaaa ttgaacaatc tcaacgacaa gaacgggtag cggcctggaa tcgtcgcgct
     2521 gaatgcgatc ttgccgcttt ccagaactcg ccaaagcaaa cctaccaggc tgaaaaagcg
     2581 cgcgatcgca aactgtgcgc caacctggaa gaagcgattc gtcgctctgg tttacaggac
     2641 ggcatgacgg tttccttcca tcacgctttc cgtggcggtg acctgaccgt caatatggtg
     2701 atggacgtca tcgcgaagat gggctttaaa aacctgaccc tggcgtccag ctccctgagt
     2761 gattgccatg cgccgttggt agaacacatt cgccagggcg tggttacccg catttatacc
     2821 tccggcctgc gcggtccact ggcggaagag atctcccgtg gtctgctggc agaaccggtg
     2881 caaatccact ctcacggcgg tcgcgtgcat ctggtacaga gcggcgaact gaatatcgac
     2941 gtggctttcc tcggcgtccc gtcctgcgat gaattcggta atgccaacgg ctacaccggt
     3001 aaagcctgct gcggctccct cggctatgca atggttgatg ccgacaacgc aaagcaggtc
     3061 gtgatgctta ccgaagaact gctgccttat ccgcataatc cggcaagcat tgagcaagat
     3121 caggttgatt tgatcgtcaa agttgaccgc gttggcgatg ctgcaaaaat cggcgctggc
     3181 gcgacccgta tgaccactaa cccgcgcgaa ctgcttattg cccgtagcgc tgcggatgtg
     3241 attgtcaact caggctactt caaagaaggt ttctccatgc aaaccggcac cggcggcgca
     3301 tcgctggcgg taacccgttt cctggaagac aaaatgcgta gccgcgatat tcgcgccgac
     3361 ttcgcccttg gcggtattac cgcgacgatg gttgacctgc acgaaaaagg tctgatccgc
     3421 aaactgctgg atgtgcagag ctttgacagc catgctgcgc aatcgctggc ccgtaacccc
     3481 aatcacatcg aaatcagcgc caaccagtac gctaactggg gttcgaaagg cgcatcggtt
     3541 gatcgtctcg acgtggtggt actgagcgcg ctggaaattg acacccagtt caacgttaac
     3601 gtgctgaccg gctctgacgg cgtactgcgt ggtgcttccg gtggtcactg cgataccgcg
     3661 attgcctctg cgctttccat catcgtcgcg ccgctggtac gcggtcgtat tccgactctg
     3721 gtggataacg tgctgacctg catcaccccg ggctccagtg tcgatattct ggtcacagac
     3781 cacggtatcg cagttaaccc ggcacgtccg gaactggcag aacgtctgca ggaagcgggc
     3841 attaaagtgg tttccattga gtggctgcgc gaacgtgcgc gtctgctgac cggtgaacca
     3901 cagccgattg aattcaccga ccgcgtcgtt gccgttgtgc gttaccgcga tggctcggtg
     3961 atcgatgttg tgcatcaggt gaaggaataa gccatgcacc tgcttcctga actcgccagc
     4021 caccatgcgg tgtcaattcc cgagctgctc gtcagccggg atgaaaggca agcacggcaa
     4081 cacgtctggc tcaagcgcca tcctgttcca ctggtctcct ttaccgtggt tgcgcctggg
     4141 ccgattaaag acagcgaggt cacacgccga atttttaatc atggcgtgac agccttgcgt
     4201 gccttagccg caaaacaggg ctggcaaatt caggagcagg ctgcactggt ttccgccagc
     4261 gggccggagg gcatgttgag cattgccgcc ccggctcgcg acctcaagct cgccaccatt
     4321 gagcttgaac atagtcatcc tctcgggcgg ttatgggata tcgatgtcct gacgcccgaa
     4381 ggcgaaattc tctcccgccg cgactattca ctgccgcctc gccgctgcct gttgtgcgag
     4441 caaagcgcag ccgtctgcgc gcgtggaaaa acccatcaac tgaccgattt actcaaccgc
     4501 atggaggcac tgctgaacga tgtcgatgcc tgcaacgtca actaaaacca caaagcttgc
     4561 gacgtcatta atcgatgagt acgccctgct gggctggcgc gccatgctga ctgaagtcaa
     4621 tctgtcaccg aaaccaggcc tcgtggatcg cattaactgc ggtgcgcaca aagatatggc
     4681 gctggaagat ttccaccgca gcgcgctggc gattcagggc tggttacccc gtttcattga
     4741 atttggtgcc tgtagtgcag aaatggcacc ggaagcggta ctccacggat tacgcccaat
     4801 tggtatggct tgcgaaggtg atatgttccg cgccactgcg ggcgtaaaca cgcataaagg
     4861 cagcattttt tctttagggc tgctatgtgc ggcaattggc cgtttgcttc agctcaacca
     4921 gccggtaacg ccaacaaccg tttgttctac ggcggcaagt ttctgccgtg gtctgaccga
     4981 tcgcgaactg cgtaccaata attcacaact gacggcaggt caacggttgt accagcagct
     5041 tggccttacc ggcgcacgcg gtgaagccga agcgggttat ccactggtga tcaaccacgc
     5101 cttgccgcat tacctcactc tgctggatca ggggttagat cctgaactgg cattgctcga
     5161 taccttgctc ctactgatgg cgatcaacgg cgataccaac gttgcttcgc gcggtggcga
     5221 ggggggcctg cgctggctac agcgcgaggc gcaaacatta ttgcaaaaag ggggcattcg
     5281 aacccccgcc gatctcgatt atctccggca gttcgacagg gagtgtatcg aacgaaatct
     5341 cagtccaggc ggcagtgccg acctgctgat ccttacctgg tttttagcac agatttaatt
     5401 atttaagcac ttgataaatt tgaaaatatt aattttcgga gaacccgtat gtctttagca
     5461 aaagataata tatggaaact attggcccca ctggtggtga tgggtgtcat gtttcttatc
     5521 cctgtccccg acggtatgcc accacaggca tggcattact tcgctgtgtt tgtggcaatg
     5581 attgtcggca tgatcctcga gccaattccg gcaacagcga tcagttttat tgcggttact
     5641 atttgcgtta ttggcagtaa ttacctgctc tttgatgcca aagaattagc tgacccagcg
     5701 tttaatgcgc aaaaacaggc gctgaaatgg ggtctggctg gtttttccag caccacggta
     5761 tggctggtat ttggcgcatt tatttttgca ttagggtatg aagtttccgg gttaggtcgt
     5821 cgcattgccc ttttcctggt gaaattcatg ggcaaacgca cgctgacgtt gggttatgcg
     5881 attgtcatta tcgacattct gctggcaccg tttacaccgt ccaacaccgc gcgtaccggg
     5941 ggtacggttt ttccggtcat taaaaacctg ccgccgttgt ttaaatcatt ccccaacgat
     6001 ccgtccgcgc gtcgtattgg cggctatttg atgtggatga tggtcattag taccagtctg
     6061 agttcgtcca tgtttgtcac cggtgcggca ccaaacgtgc tgggtctgga gttcgtcagc
     6121 aaaattgccg gtatccagat tagctggttg cagtggttcc tctgcttcct gccggttggg
     6181 gttatcttgc ttatcattgc gccgtggctt tcctacgtgc tgtacaaacc ggaaatcaca
     6241 cacagtgaag aagtggcaac ctgggcgggt gatgaactga aaaccatggg tgcgctgaca
     6301 cgcagagagt ggacactgat tggccttgta ttgctcagct taggtttgtg ggtttttggc
     6361 agcgaagtca ttaatgctac tgcagttggt ctgctggcag tttcgctaat gctggccctg
     6421 cacgttgtgc cttggaaaga tattacccgc tataacagcg catggaacac gctggtcaac
     6481 ctggcaactc tggttgtgat ggctaacggc ctgactcgtt ctggctttat tgactggttc
     6541 gccggtacca tgagtacgca cctggaagga ttctcaccaa acgcaacggt gattgtactg
     6601 gttctggtgt tctactttgc acactacctg tttgccagcc tgtctgcgca caccgcaacc
     6661 atgctgccgg ttattctggc cgtcggtaaa ggtattccgg gcgtaccaat ggaacaactg
     6721 tgtatcctgc tggtgctgtc tatcggtatc atgggctgtc tgacgccgta tgcaaccggt
     6781 cctggggtga ttatttacgg ctgtggctat gtgaaatcaa aagattactg gcgtcttggc
     6841 gcaatcttcg gggtgattta catctctatg ttgctgttgg ttggctggcc gattctcgcc
     6901 atgtggaact aatacgttcg ccaaacgctg aataaatcat cacgcccgcc aggtaagccc
     6961 acctggcggg cttttttatg atttaataga tagtattcag tgtatgtatg agttccacac
     7021 ccattatgaa agcattctgg cgtaacgccg cgttgctcgc ggtttctctg cttcccttct
     7081 cttctgccaa cgccttagcg ttgcaggcaa aacagtatgg cgattttgat cgctatgtcc
     7141 tggccctctc ctggcaaacc ggattttgcc agagtcaaca cgatcgaaat cgtaacgaac
     7201 gagatgaatg tcgcctgcaa accgaaacga ccaacaaagc tgattttctg accgtacatg
     7261 gtctgtggcc aggattgcct aaatcggttg ctgcccgtgg tgttgatgaa cgccgctgga
     7321 tgcgcttcgg ttgcgctact cgcccaatcc cgaatctacc agaagcgcac gccagccgga
     7381 tgtgttcatc gccggaaacc ggattatcac tggaaacggc cgctaaacta agtgaagtca
     7441 tgccaggagc tggcggacgt tcctgcctgg aacgctacga atatgccaaa cacggtgcct
     7501 gctttggttt tgatccggac gcatacttcg gtacgatggt acgcctgaat caagaaatta
     7561 aagagagcga agccggaaaa ttccttgcgg ataattacgg taaaacagtg agccgccgtg
     7621 actttgacgc cgcctttgcc aaaagctggg gaaaagagaa cgtgaaagca gttaagctaa
     7681 cgtgccaggg taaccctgcg tatttgactg aaattcagat ctcgatcaaa gctgacgcaa
     7741 tcaacgctcc gctttctgca aactcattct tgccacaacc acacccaggc aactgtggca
     7801 aaacctttgt gattgataaa gtgggttatt aatcatctga tttacaaaac acgctaattc
     7861 gtcttccata aaccgtgacg attaagaatt atacataatg cgctgcattg aggaattccc
     7921 tgaatgtgac gcaaatcact tcaaagagtg agcaacagtc agtatcatca tgtgagttaa
     7981 accctcgccg cctgacggtg agggttttct tttgggtatg tttatctgcg acactcgcag
     8041 taccgacgac atggagtaaa aatgtccaga ccaactatca tcattaacga cctggatgcc
     8101 gaacgcatcg atattctgct ggagcaaccc gcctatgctg gtttgccaat cgccgacgcg
     8161 ttaaacgcag agttggatcg cgcccaaatg tgttcgccag aagagatgcc acacgacgtg
     8221 gtgacaatga acagccgggt taaattccgc aatcttagcg atggcgaagt gcgtgtgcgc
     8281 acgctggtgt atccggcaaa aatgaccgat agcaatactc agctttccgt tatggctccg
     8341 gtaggtgccg cactgctggg gctgcgcgtt ggcgattcca ttcactggga acttccgggc
     8401 ggcgttgcaa cccaccttga agtgctggaa ctcgaatacc agccagaagc tgctggcgac
     8461 tacctgcttt aatcccatct gcacaagcga taactcgctt tgcccagagg atgcactcgc
     8521 atcctcttta cgcaaaatca gcaatttttc ctggttctca ctgttcactt ttgctgcaaa
     8581 attattccgt ttcgtttgtg ggaataacca tggcagaagc attttacatc ctgataggat
     8641 ttctgattat ggcggcgatt atcgtcatgg ctgtccttta cctcgaaaac cactcctgac
     8701 gatcatattg ttccccccgg catcgcattc accagacctg aaaccgcctt ctgcgccgcc
     8761 tctgcgcttt gtgcaccggg taccagaatc accttacggc actcctcttc acgtttttcg
     8821 aaaatctcat atccccgggc ggcctcttca aacggcatat agtgggtaac aatttcttct
     8881 ggtttcagta atcctttctc aattaacggt aataattctc ccagccatgc gtgaacgtgg
     8941 gtctgtccca ttttaaacgt caacccttta tcaaaggcgt cgccaaacag gaaaccgtga
     9001 ataaatccag cgtagacgcc cggtacgcta acaatgccgc cacgcctgac cgccgcaata
     9061 cactggcgca acgctttacc gctgctgccc tccagtttca ggttagtcag caccgtttcc
     9121 gtggtgctgc ctttcgcttc aaaaccgacg gcgtctatta ctgcatccac gccccggtga
     9181 cctgccgttt gttcaataat tgactgtgcc ggatcgctat cttcatcgaa attaatcggg
     9241 atcgcgccgt agcggtcagc ggcgaaacgc aagcggtagg gatgatgatc aacaacaaaa
     9301 atctgttccg caccgagcaa ccgtgcacag gcgattgtca acaatcccac tggaccggca
     9361 ccatagactg caacgcttga accttgttgg atctgcgcat tttttgctgc ctgccatgcc
     9421 gttggcagaa tatcagaaag gaaaagggct ttatcatctg aaagcaaagg cggtacttta
     9481 aacggcccca cattcccttt agggacgcgg acatattccg cctgtccacc aggaacgcca
     9541 ccatacaggt gactataact aaacaatgcc gcgggagctg gtatctgttt tttattgagc
     9601 gcagcgcctt tacccgcatt ggtattttcg caggcggcat attgctgcat tcgacagaaa
     9661 aaacagtcgc cacaagcaat gacaaacgga attaccaccc ggtcgccttt ttgcaaattt
     9721 tttacgtcct ttccggtttc aacgacttcc cccataaatt catgaccaaa aatatcgcca
     9781 tgtttaacct ggggtatttt gcctcgataa agatggaggt cagagccaca gatcgccgtt
     9841 gccgtaatac gcagaataat atcatctgcc tgttcaatgc ccggatcggg aacattttct
     9901 acctgaacgt gatgtgggcc gtgataagtc aatgctttca tgcaacctcc agagacagtt
     9961 caaaaaagag tcagtcttta agggtagcta tcacagcggg aaaacgggcg gtaccgcagg
    10021 ttttgggaga attcctggtg ttcagtgaga atatattaat cacagaaacc gttcgtaagg
    10081 aaaaagaacc agacaaatat cagggataaa tattataatc atggcgaatg atttgttcat
    10141 gattaacagg gagaaagggt tatgtataag acaatcatta tgccagttga tgtatttgaa
    10201 atggaattga gcgacaaagc tgttcgccac gctgaattcc tcgcccagga tgacggagtt
    10261 attcatctac ttcacgtact acccgggtca gccagcctga gcctgcaccg ttttgccgct
    10321 gatgtgcgtc gttttgaaga gcatctgcaa catgaagcag aagaacgtct gcaaacgatg
    10381 gtcagccact tcaccatcga tccttcccgc attaaacaac atgtccgttt tggtagcgtg
    10441 cgggatgaag tcaatgagtt ggcagaagaa ctgggggctg atgttgtagt tattggttct
    10501 cgcaacccat cgatttcgac ccatctgtta ggttctaacg cctcgagcgt gatccgccat
    10561 gccaatctgc cggtgctggt tgtgcgttaa ttgccagaat aaatatcccg ccctgcccgg
    10621 agccatcccg gcagggcttt ttaatgccat aaaaaagccg ccaggtttga ccccggcggc
    10681 taagcaattg caggtgaatc ttacttcttc ttatgcagtt ttggtgcgaa tcaggtagtc
    10741 aaaagcactc agagaggctt tggcaccttc gcctgtggcg atgataatct gcttgtacgg
    10801 aaccgtcgta cagtcacccg ctgcgaacac gcctttgacg ttggtttcgc atttcgcatc
    10861 aatgataatc tcacccatgc ggttacgttc gactgcgcct tccagccagt tggtgttcgg
    10921 cagcagacca atctggacga aaataccggc cagttcgatg ttgtgaatat cgccgctgac
    10981 acgatctcga tattccagac caacgacttt gctgccgtcg cctttcactt ccgtggtttg
    11041 cgcattcaga ataatgtcga cgtttttcag gctgcgcagt ttgtcctgca gaacctggtc
    11101 ggctttcatt tctggcgcaa attcgagcag cgttacgtgc tcaacgatac ccgccaggtc
    11161 aattgccgct tccacgccgg agttaccgcc gccgataacc gctacgcgtt tgcctttaaa
    11221 cagcgggccg tcgcagtgcg ggcagtaggt cacgcctttg gtgcgatact gatcttcgcc
    11281 aggaacgttc atgttgcgcc attttgcacc ggtcgccacg ataatgctgc gtgctttcag
    11341 taccgcgcca gaagctgttt caatctgatg cagaccacct tcaactgctg ctgggatcag
    11401 tttgctggcg ctctggctgt cgatcacatc aacgtcgtat tcatcaacgt gaactttcag
    11461 tgcgcctgcc agcttctgcc cttcagtctt cggtacagaa atgtagtttt cgatatcaac
    11521 ggtatcgagg atctgaccac caaaacgttc gcccatcaga ccggtacgga tgcctttacg
    11581 tgcggagtaa attgctgcag ctgcacccgc cgggccggaa ccaacgatta atacgtcata
    11641 agcatcacgc ttgttcagct cttctgccgc acgtttttcc gcgcccgtat caattttggc
    11701 aacgatttca gtcaacgtca tgcggccctg accaaactct ttcccgttta cgaacactgc
    11761 cggaacgccc atcacgttgc gatcggtgat ttcgttctgg aaggtgccgc cgtcaattgc
    11821 agtgtgcttg atgcgcgggt tcagtacgct catcaggttc agcgcctgca ccacgtccgg
    11881 gcagttatgg caagagagcg agtaataggt ttcgaattca aaatcaccgt caatatggcg
    11941 aatctgctcc agcagagact gcgcttcttt cgacggatga ccaccggtcc atagcaacgc
    12001 cagtaccagc gaggtgaact cgtggcccag cggggagcct gcaaaacgtg gcccctggtt
    12061 ggaacctggg ttggtgatca ggaaagacgg cttacgcacc ggcaagctgt tatcttcttt
    12121 aaaggtgact ttgtctgaca gttctgcgat ttcagccaac agttccttga tttctgccga
    12181 tttagcgctg tcatccagcg tggcaattaa ctcaacaggc ttggtcaatt tctcaaggta
    12241 agccttgagt tgagttttca tatttgtgtc gagcatgttt atctcctggg ctttaaacat
    12301 catcatgcaa gcggcctctg cggctacctg gatgcagctt gcatcatggt gcggggaaga
    12361 aaatcgggtg ccgcagcacc cgaagaatta ggtgaatttc cgaagtattt cgcgccggag
    12421 ttggtagcgc gaaagacgaa ggaaatttag attttaccaa ccaggtccag agacggagcc
    12481 agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg agaagctacg
    12541 tactgtgctg ctttgatttt acgcagcagg tcagacgcgt cacggccaat gccttcagcg
    12601 gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc
    12661 agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg
    12721 atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg
    12781 aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag ttcttcgtag
    12841 tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc cgggtagaag
    12901 aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt
    12961 ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat ctatacttcc
    13021 tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta atgcgtttcc
    13081 attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt gaactttgca
    13141 ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt aatctgaact
    13201 accctttggt atcttcagtg cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca
    13261 acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta agctcttacc
    13321 tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa aggacaaatt
    13381 aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg cagaggaact
    13441 tcctgctcca gtaaaagcga ttgaaaaaca gggcattaca atcatcaaaa cattcgatgc
    13501 ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca ccatctacct
    13561 gactccagat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag gtgaaaacct
    13621 gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg
    13681 gatggaacaa tcccactggc tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt
    13741 gttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc gcccgtgggt
    13801 agattctggc aaagtgcaat taagaacatt gttggttggg gttatcaagc cagaaagccc
    13861 ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc aagaatatga
    13921 atcctctggt ggcaagctta agctaaacgt gcctgcaaac gtaagtacag aacaaatgaa
    13981 agtgttaagc gccaacgaga aactcatgga cgatctgggg gcaaatgtca caccagcgat
    14041 ctattacatg agtaaggaaa atacgctgca acaggccgtg gggttgcccg atcagaaaac
    14101 gcttaatatc attatgggga ataaataaaa gataattcat ggatggttga aatacatccc
    14161 tgcttttcaa ccatcctaaa aaagcatgaa ttgctcgctc tgccctaagt ggctatgcta
    14221 ttttagaaaa acttaatcct gagttaagca aaaaaatata atcaatgaaa atatatacgc
    14281 aaaaaacatt gattaagtga atataccatg gaagaaaaat ataaccggag tagtgtatgg
    14341 ccaatctcta cgacttgaaa aagttcgact taaaccttct tgtcatattt gagtgtattt
    14401 accagcattt aagcattagc aaagctgctg aatcactata tatcacccca tcagccgtga
    14461 gccagtcgtt acaacgctta cgtgcgcaat ttaatgaccc tttattcatt cgctcaggaa
    14521 aaggtatcgc gccgacgaca acagggttga atctgcatca ccatcttgag aaaaacctca
    14581 aaggccttga acaaacaatt aatatagtca ataagtccga actaaagaaa aactttatta
    14641 tttacggccc tcagctcatt tcttgctcta ataatagtat gctgatccgc tgcctacggc
    14701 aagatgcctc tgtcgagatt gagtgccatg atatcctcat gtcagcagag aatgctgaag
    14761 aactgttggt tcaccgcaaa gccgatctgg tgatcaccca aatgccagtg atcagccgtt
    14821 ccgtcatttg catgccattg catacaattc gcaatactct aatttgtagt aataagcatc
    14881 cgcgaattac tgacaatagc acttatgaac aaattatggc agaagagttc acccaattaa
    14941 tatccaaatc agcaggagtt gatgatattc aaatggaaat agacgagaag tttatgaatc
    15001 gcaaaatttc atttcgtggt tcctctttgc tgacaatcat taatagcatt gcagttacag
    15061 atttactggg aatagtcccc tatgagcttt ataattccta ccgtgacttt cttaatttaa
    15121 aagagataaa actagaacat ccgttacctt caataaagct ttacatttcc tataataaat
    15181 cttctttaaa taatttggtg ttctcccgat ttattgaccg tctaaatgag agtttttgat
    15241 atataactac agacagctac tataactttc atctatttat tcacagcgcg aatgaaaaat
    15301 aaaacactct gctttgcagt gtttacttta taattaacag acttatctca ctgatcaccc
    15361 tgtaacgttc agagagcgtc ttcagatgtc tatttataaa attcctcttc cgctcaatat
    15421 tctggaagct gcacgagagc gtattacctg gacactcaac accctacccc gcgtttgtgt
    15481 ctcattttct ggcggcaaag actctggttt aatgctgcat ctgacagccg aacttgcccg
    15541 acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat tctcttgcac
    15601 cattaactat gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag agttttactg
    15661 ggttacgctc ccgcttacga cgcaaaattc cctttcacaa taccaacccg aatggcagtg
    15721 ctgggaacct gatgtcgaat gggtgcgtca gcccccgcaa gatgcgataa ccgatcctga
    15781 ctttttctgc ttttaccagc caggcatgac cttcgaacaa tttgtacgtg agtttgccga
    15841 atggttttca caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg atgagtccta
    15901 caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg ataaaccatg
    15961 gactacggcc gcaccaggcg gtcatagctg gtacatttac cccatttacg actggaaagt
    16021 ggctgatatc tggacctggt atgcaaatca tcaaaatctc tgcaacccac tgtataacct
    16081 aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt ttggcccgga
    16141 gcaacgacaa ggattatggc tctatcacgt tatcgaaccg gatcgctggg ctgctatgtg
    16201 cgcacgagtc agcggggtaa aaagtggcgg catttacgcc ggacatgata atcatttcta
    16261 tgggcatcgg aaaatcctca agccagaaca tttagactgg caagaatatg cattattgct
    16321 gctcaatagc atgccggaaa aaacagctga gcattaccgc aataaaatcg ccatttattt
    16381 gcactggtat cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg acattggcgc
    16441 gaaagatatc ccctcctggc ggcggatatg caaagtttta ctcaataacg attactggtg
    16501 tcgggcatta tcatttagtc ctacgaaagc gaagaactat cagcgttata acgaacggat
    16561 aaaaggaaaa cgtcaggaat gggggatact atgcaacaac gattaacgca ggatttaacg
    16621 caatttctcg ccagcttgcc ggaagatgat cgcatcaaag cgattaatga aatccgcatg
    16681 gcgatccatc aggttagccc ctttcgtgaa gagccagtgg attgcgttct gtgggttaaa
    16741 aacagccaac ttatgcctaa tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa
    16801 ctgctgaaaa aatccattga aatagatggt tttacccaac ccattgtggt cacgcatacg
    16861 gataaaaatg ccctggaaat cgtggatggt tttcaccgcc atgaaattgg taaaggctca
    16921 tcatcattaa aattacgctt aaaaggttat ttgccggtga cctgtctgga aggtacgcgt
    16981 aatcaacgca tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc
    17041 gccatgtcgg aaatcgtccg tgaacttagc cagttgggat gggacgataa taaaattggc
    17101 aaagagctag gaatggacag cgacgaagtg ctgcgcctga agcaaattaa cggcctgcaa
    17161 gagctgtttg ccgaccgtca atattcccgc gcctggacgg ttaaataact aaagctggcg
    17221 caggcgttca gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag
    17281 tttatgtggg aagggatcgg cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc
    17341 ctgcgtcagc cactggcaaa actcaacatc atccagggta gaaacctcgc tgtaatccac
    17401 cagcaaaaag tatgtacctt cacacggtaa aatctccagc cggctttcat ttaaagcatt
    17461 caccagaata tcgcgcttct ggcgataaaa gtccggtaac gcaagataat gctcaggttc
    17521 tgcacgtagc atatcagcaa gcgccagctg tgccggggta ttcaccgaaa aggtcagata
    17581 ctgatgtacc ttgcgaattt cggcgctgat gggcgctggc gcaacacaat aacccacttt
    17641 ccagccggtc atatgatagg tcttgccaaa tgaagaaacc gccactgccc gctcacgcag
    17701 ctgcggatgc gccagcacac tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac
    17761 ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc
    17821 agcctgctgc cagacagttg cactggggtt atgcggagtg ttgaggatca ccagtctggt
    17881 gcgctcgctt aataatgcgg caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag
    17941 tgccatacgc ttcactattc ccccagaaag cgcgatggcg ggggagtaac tgtcatagct
    18001 gggatcaaaa caaatcactt catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa
    18061 cgcttccgtc gcccctgccg ttacggtgat atcgctatcg gcatctggtt gatagccata
    18121 caaacgttcc gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc
    18181 gtattggttt gccccctggg caacgtggta cgccagccgc tcctgtaaat agcgcggacc
    18241 atcaaaatca ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct
    18301 catctgggtg aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa tcagagggtt
    18361 atttgtcatt tttattgtac ctgacgaagt tgcgtgttga aggcactata acacgatgct
    18421 agtatttggc aatcaagacg tttagatgtc taaataaaac aataaggaca acacaacatg
    18481 cctcacaatc ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt
    18541 ttcaatcacc tgcacgattt tttcactgat gaacaacttt ctcgcgcggt gtggatctac
    18601 ggcaaacgcg ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg
    18661 gcaaagcata ttttgtttcg tggtcattgc agcgaaagcg atgtacagca actggcggct
    18721 gagtccggtg acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc
    18781 gcgaaagccc tcgcccgccg tctcggtctg ccgtttgttg ccgttccgac gatcgccgcc
    18841 acctgcgccg cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat
    18901 tatgagattt tcgacgacgc caattttatg gtgctggtgg aaccggagat tatcctcaat
    18961 gcaccgcaac aatatctgct ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg
    19021 gtggtgctgg ctccgcaacc agaaacgttg ccgctaaccg tgcgactggg gatcaataat
    19081 gcgcaggcca ttcgcgacgt attgttaaac agtagcgaac aggcgctgag cgatcagcaa
    19141 aatcaacagt taacgcaatc attttgcgat gtggtggatg ctattattgc tggtggtggg
    19201 atggtcggtg gtctgggcga tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac
    19261 ggtctgactg tcctgccgca aaccgagaaa tttctccacg gcaccaaagt cgcttatggc
    19321 attctggtac aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg
    19381 tatcagcgtt ttcatctgcc gaccacgctg gcggagctgg aagtggatat caataatcat
    19441 gcggagatcg acaaagtgat tacccacacc ctgcgtccgg tggaatccat tcattacctg
    19501 ccagtcacgc tgacaccaga tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa
    19561 gcctgacgtg caaatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg
    19621 ccgctcacgg aaaaactctt cgtaggtcat cggcgtttga tcgggatggt taacccgcat
    19681 atgttcgaca tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa
    19741 atattttccg gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga
    19801 aagagtgccg gatgcggcgt gaacgcctta tccggcctac aaatgctatc aacgcgctcg
    19861 gtcagtcccc tcgccccatc ggggagaggg ttagggtgag gaggaaccct cggcactatg
    19921 ccaacattcg ccaacatccc ccctcactct gactttagtg tgcgcctttt gcctgtgcca
    19981 cgatctcctc gacattttcc ggcattggct catacggcgt ttctttcgcc gttggcttcg
    20041 gatctttcaa tgccgccagt gccgtcttaa tcgagaacag cgccagaacc accacgacca
    20101 ccataaagaa gatggttaac ccggcatcca gacggttgtt gaacaccagt tgcgccagct
    20161 gtgactcagt atactgcgac ggaatattgc cgctgtcgat cattgcctgg aacttattag
    20221 caatggccag gaagccgact ttcgcatccg ggctaaacgc tttctgccag cctgcggtca
    20281 gggtacaaat cagcagccag gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt
    20341 tcatcttgaa caacaccacg gcacagagca tcagcgccat ccctgccagc atctggttgg
    20401 caataccaaa cagcggccac agagtgttaa tgccgcccag cggatcgacc acgccctgat
    20461 ggaggaagta gccccacgcc agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg
    20521 aatcggtccg cttcaggccc ggagaaacca cgcccagcag atcctgcaac ataaagcgcg
    20581 cagcacgcgt acccgcatct accgccgtca gaataaacag agcttcaaac agaatggcga
    20641 agtgatacca gaacgccaca tccatcatac cacccagcgc accgtgcagg atgtaagcca
    20701 tccccaccgc cagcgtcggc gcaccgcccg cacgggaaat gatcgactgt tcacccactt
    20761 cgctggcaat ctggttgagc gtatctggtg taatgctaaa gccccagcta ctcaccacct
    20821 gcgcggcaga agcgactaca tccgccgtcc ctgccggagc cagcaccgcc attgggctgt
    20881 tcatggcaaa atacacgccc ggatcgatga tacaggcaga aaccagcgcc ataatcgcca
    20941 cgaaggattc catcaacatc ccaccgtagc cgataaagca cgcctgccct tcgttcgcca
    21001 gcatcttcgg cgtggtgccg gaagagatca gcgcatggaa gccagacacc gcaccacagg
    21061 cgatggtgat aaacaggaac gggaacaggt taccggtcca taccgggcca gtgccatcga
    21121 caaatttggt cagcgcaggc atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca
    21181 gaccgacgat agtcccgatt ttcaggaagg tcgagagata gtcacgcggg gccagcagca
    21241 accacaccgg cagcaccgcc gccacaaaac cgtagcccac cagcatccag gtcagctgca
    21301 cgccggtaaa gtcaaagtac ggtgcccagg tcggactttc tgccacccag ccgccagaga
    21361 taatggcgaa aatcaggaac accaggccaa tcactgacac ttcgccaatg cggcccggac
    21421 gcagatagcg caggtagatt cccataaaaa tcgccagcgg aatggtgaac gcaacggtat
    21481 acgttcccca cgggctatga gtcagggctt tcaccacgat catcgccagt actgcaagga
    21541 taatgaccat gatcataaag caggctacca gcgcaatcac cccggcagtt ggccccatct
    21601 cttctttgac cagctcaccc agcgatcgac cgtcacggcg cgtagaaaca aacagcacca
    21661 tgaaatcctg caccgcacca gcgagaacca ccccggcgag cagccagatc atccctggca
    21721 ggtaacccat ttgcgccgcc agtaccggcc caaccaacgg acctgctccg gcaatggccg
    21781 caaaatggtg accgaacagc actttcttgt ccgtcggcac atagtccagc ccgtcgttat
    21841 ggcgcaccgc tggcgtcata cgcgtcgggt caaccgccag cacatttttg gcgatataca
    21901 gcccataaaa acggtaagcg atcagataga tacagaccga cgccaccaca atccacagcg
    21961 cgttgatctg ttccccacga tttaaagcaa tgtatcccag agcaaatgct cccattacag
    22021 agagcactgt ccagacgagg tatttccctg atttgttcat agttgttatc cgtgtgcgtg
    22081 tccatagaga tgttacattt tgtttctata acatctttgt aagatttaac aacacggtaa
    22141 ccatgtaaat cggagttgaa accaatattt aacttaacaa tgttaactcg atcactccgt
    22201 taaccgagtt ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa actgccgtac
    22261 ccagccgaca agtgcagcaa cgccgcccct gttcatcgaa aacgacgatt tcccagctct
    22321 gattttgccg cccaagatgc agcggctggc agacgccgcg taccttgcct tcagacaccg
    22381 ggcgatgatg ggtggcgtta agttccgtac ccaccacaca ctgcccgtca cgggtcatca
    22441 taaatccggc catcgatccc agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca
    22501 ggccaaacgg ctgatgagta cgggtatcaa ccggcatttc ggcttccagc acatcatctc
    22561 ccagacgggt atacacaatt cccagatgcg ccaccattgt gttatcgctg gtggcgttca
    22621 gttcgtcgag cgttaaatgg cgtttccaga tcatgcttat gcccccagcg ttgagccgcc
    22681 atcgaccaca atatcctgta gggtgatatg gctggcgagg tcagaggcga ggaacaaaat
    22741 cgtgttggcg atctcttgtg gacgggcgat tttccccagc ggaatgccga gtttaaactg
    22801 ctcgccaaag ccgcgaatac gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg
    22861 catatcggtg tcggtggagc caggcgaaac cacattacag cgcacgccgc tacccgccag
    22921 ttccagcccg acgctcaacg ccaggctttt cagcgccgct ttcgatgcgc cataagcact
    22981 cataccaata cgcggcgtgt gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg
    23041 ctgacggcga aactggttca tggtttgctg gaacaggtta aacgcaccgc cgacgttaac
    23101 cgcaaaagtc tgctgccagt cctctttact gagctgatcg gtcgcgccca tgcgcaaaat
    23161 tcccgccgca ttgatcagca cgtccagtcg ctccgtttca gctaacagtc gctgacatac
    23221 ctgcgcgacc tgcgcagcat cggcaacatc catcacttcg gtcgcaaagg gatattgctc
    23281 ctgagcgaac gcttgatcaa aaccggtaac tttcgctccc gcctcaacaa acgccagcgc
    23341 cgtggcgtag ccgatacctt tacctgcgcc ggttacccag acatttttac cgctgaaatc
    23401 cattatttca cctcgcggga gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc
    23461 agcatgacaa agtcgatatc accatgcact ttgcgccagc gcgccgccag cgccatcatg
    23521 cgcaccgaat ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg
    23581 tccagcaacg gcaggataac ctcacgcagc gccgctttgc tggcggggac aggtgctggc
    23641 agtaattctt cagtcatcac cacccggcca gaccgcccgg ccacatattt cagcgacatc
    23701 aaatgctcgt cacggctgaa atcggccagc gcatccgcca ccataaacgg tttaatatcg
    23761 cgcataaatg cgtcggttgc ggtggtcata cagccaatgt gggcatatac cccggtaata
    23821 atcagctggt tacgtccact ctctttgagc atttgctcca gcggagaacg atgaaacgcg
    23881 ctgtagcgcc acttcaccag caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc
    23941 ttttgctgtt ccggcgaacg ggtcaggccc ggcccccaca tatcattcaa cagcgcccga
    24001 tcttcatcgc tctgctcttt cggctgggcg gtgtaataaa ccggaatatt gtgctgtttg
    24061 cagtagtcgc gcagcgcagc aatattcgcg atcacctgct ccatcatcgg gcagttctcg
    24121 ccccagaagc tgacaaaata gtcctgcata tcatggatta acaacgcggc acgttgcggt
    24181 tcaaaggccc agtcaacttt attcttaggg atatcgtgag actccggcag tgcgtaagcc
    24241 tgtaattttg gaatagccat cgtgttctct ccttcaggct gatgcgcgtg acgccagcca
    24301 ctgacgtaat tgttttttat cgactttccc gaccgccgtc agcggaagtg aatctacaca
    24361 ctccacgcga tccggtaatt taaattcggc aataccctgt tcacgcagga aacgacgcac
    24421 ctgcaccgcg cgcagcggct cttttaccac cagataagcg cagctttttt cgcccatcag
    24481 ctcatcttcc atgctcacca gtgcggcgta gatcaccgcc gggtggcgca gcagcaggtt
    24541 ttcgatctct tcggcagcga tcttctcacc gccacggttg atctgatctt tctcgcgccc
    24601 ctgcacggtg atgtacccct ctggatcaat agagatcaga tcgccggaac agtaaaaacc
    24661 gttggcatca aaggcgctgg cattgtgctg cgggcttttg taatagccac ggaaggtgta
    24721 cggaccgcgg gtcatcaggc gtccaacttc cccttgcggc agtggatttc cttcggcatc
    24781 ggcaacccac acttcatcgt ccggacacat tgggtaaccc tgggtatgga taattttctc
    24841 cgcgctatca tcaagacggg tgtagttcac cagcccttcc gccatgccaa acacctgctg
    24901 caactggcag ccaatctcag cgggaatacg cgccgcaagg gtggcagaaa gacgtgcgcc
    24961 gccgacctgt aacagtttca gcgaggcaag ctgcgcccgg ctttcacctt cggtcagcgc
    25021 ctgcaaccac aggctgactg ccggtggcac cagcgcggtg acgttcacct gatgtttttc
    25081 aatcaatggg aagcaaagcg tggcgctggg atcggccgcc agaacaaccg ttcctccggc
    25141 aagaaagacg cccagcgatc ccggcgaact catggcgtag ttatgagccg ccgggatcgc
    25201 gcacaggtag cgtgtctgtt gtgtgaactg acaaatctcg acgctacgac gcacgctgta
    25261 gtagtagtcg ttatgagtgc gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag
    25321 ctggaaatag gccacttcat cagcaggtga tggcgtggca gtaaaatcct cagccggatg
    25381 gttaatcgca tcctgcaagt tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat
    25441 ggaggaatgt tctgtgacga aagtattgag gaaatcatcc ccgctaaaca gcgcatgttg
    25501 gcgatcggca atcagcaatg cgggttcaat ctggctggca taggcgttca gttcactacg
    25561 ctgatggctg aacagcgcca gcaccggcgc aacgcccagt ttcagcagcg cgaaaaaggt
    25621 aatatacaat tcagcgacgt tacccagttg taccagcgcg gtttcaccag gtttaatgcc
    25681 ctgacggcgt aaactacacg cgaggttatc cgccgcctga ttcagctccc ggtaactcaa
    25741 ctgtcgctcg ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc
    25801 ggtcagcggc aaatcctgcc agtagccttt ttcccgatag cgacgggcaa actcttccgg
    25861 ccagcgggtg aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat
    25921 ggtagaaagt ttgacgcctg tttcgcgcca ctcacccaac ggtgacgacg caggcacaat
    25981 ccccgctccg gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac
    26041 cacccattcg ccgttacctt cgctgtcaca ccaacccaca atgccgccaa acagttcgcg
    26101 gtcgaatggc tccagttcag caataacctg ggtcgcggcc tgatgcggga aaccgctcag
    26161 cgcgggggtt ggatgcagca gacaggccag agtcagtgcg ttttcttgcg aattcgcttt
    26221 accttcaaag ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt gtggagagga
    26281 gggaacgtgt aactcactac tgcgttcgcg cagtacctct ttcatcgcct gagtcaccag
    26341 ttcatgttca tggcgatctt tttctgacgc cagcagacga ttacccgctt cgcgatcgag
    26401 cacttcatcc ggctgacgac gcgcggaacc ggctaacgga atggagctaa aacgctcgcc
    26461 gtctttacgt agcagcagtt ccgggctggc ccccagcagg acgccaccat cagccagcgg
    26521 gacatggaag ttgtaactaa ccgggttttg cgcaatcaac cgttccagca atacgccact
    26581 atcaatggcg gcgtcagtgg tgatatcaat caatcgtgac aacaccactt tgtcgacctg
    26641 cggcgtggcg gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg tttgttcagg
    26701 aattgcctgg cgttccacca cgttcagcga ctggctgcgg gtaaaacggc gggctgaggt
    26761 ctgtttttcc tggcgggaga acgactgcca ggattcggga atatacagcg acgaaggctg
    26821 acgtggatcg aagggaatcg ccccgaccat caccggattt ttgatgccct gcgctttggc
    26881 atcggcaaac agcgcggcga gtttttgctg gaagggactg tcgggcgaat ccccgttcac
    26941 agccggttca tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt acggcgacat
    27001 aaagaaaaag cgattgggcg caagtgttgc catggtctgc tgtacttcct cagccagtga
    27061 cgtatccata tcatcctcca caaaatgata aaggctttaa taatgattat catttatatt
    27121 ttcggtcgct aacctaaaag caaacgcgca ctatgtcaac tcttgaggta acgcaatctg
    27181 caactcgggg ttgcatctgg ttgtggttac aatgaaaatg agaagcatta ttgatggatt
    27241 cgcataagcg caatgtgatg tcctgcgccg ttctgccccc tctcccttcc agggtgaggg
    27301 ctggggtgag ggttaatgtt cgcaccagtg ctggctgttc ccctcaccct ctccccaaag
    27361 gggcgagggg acggattgtg cgctttgtcg aatttgtcat tacgcccata accttattaa
    27421 taacaggaag ctgatttgtg agactcgccc cgctctaccg caacgccctt ctattaacag
    27481 gacttttgct ttcaggaata gccgcagttc aggccgctga ctggccgcgt cagattactg
    27541 acagccgtgg cacacataca ctggaaagcc agccgcagcg tattgtttcc accagcgtca
    27601 ccctgaccgg ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc
    27661 cgaataaccg cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag
    27721 aacgcaaact gcaacggctc tatatcggcg aaccgagcac cgaagccgtt gccgcgcaaa
    27781 tgccggatct gattttaatt agcgcaaccg gcggggattc ggcgctggca ctgtatgatc
    27841 agctttccac catcgccccg acattaatca tcaattacga cgacaaaagc tggcagtcgc
    27901 tgttaacgca gcttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcac
    27961 agtttgataa gcaactggca gcggcgaaag agcaaatcaa attaccgccg cagccggtca
    28021 ctgccattgt ctataccgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac
    28081 aagggcagat gctggaacaa ctcggcttta cgctggcgaa gttgcccgca gggttaaacg
    28141 ccagccaaag ccagggcaaa cgccatgaca tcattcagct tggtggggaa aatctggctg
    28201 ctgggttaaa tggcgagtca ctattcctgt tcgccggtga tcagaaagat gccgatgcga
    28261 tttatgccaa tccactgctc gcgcaccttc ccgcagtaca aaacaagcag gtttatgcgc
    28321 tgggaaccga gacgttccgt ctggattact acagcgccac gcaagtgctg gaacgactta
    28381 aggcgctgtt ttaagcatta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    28441 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    28501 cttgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccag cagcgccgcg
    28561 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    28621 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    28681 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    28741 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    28801 tgtgccagct tcccgctggt taacgcacca atagccgcgc cgagcgggat cgccgcgtag
    28861 agaaaaccaa tctgtgccgc tgacatctgc cagttgtcag ccagcgccgg atacagtacc
    28921 cgcaccgcgc tcgccatcgt caataaacca cccagcagcg caatcccgcc caccagcggg
    28981 ctggcaagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc acgcggctgc
    29041 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    29101 gccgccagcc cgtagttcca ggctacgcca ccggtcgcca gcaataaacc gccaatcatg
    29161 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    29221 ttttcacgcc ctaccagtgc tggtgtcgcc gccaatagcg ccgtaacgcc aagcgatgcg
    29281 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagt
    29341 gcattaaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    29401 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    29461 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    29521 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    29581 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    29641 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    29701 ctaacatatc caaataagat agataacgat aattaatttc attatcatgg aagttcgtat
    29761 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    29821 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct
    29881 ggaggccctc tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    29941 gccgcgcacc cttgccggtt tacttgcagg cggcgcgctt ggccttgccg gggcgttgat
    30001 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga atgccggagc
    30061 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    30121 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta ccggcagtca
    30181 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    30241 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gatgtctacg atcaactgcg
    30301 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    30361 cccggtgctg atcgccggag caaccgcgct attactgagc cgcgcgctga acagtttaag
    30421 cctcggcagc gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttaattgg
    30481 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    30541 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    30601 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    30661 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    30721 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggcgca tgatttacgt ctctcgccga
    30781 ttaatcatca cctgtttgct gttggtttcc gcctgtgtgg ttgcaggtat ctggggatta
    30841 cgcagcggtg ccgtcacgct ggaaacctcg caggtattcg ccgcgctgat gggcgatgcg
    30901 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    30961 ttgattggcg cggcactggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    31021 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtagcg
    31081 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    31141 atcacttcgc tgttggtctg gctgctcgcc tggcgtaacg gcatcgacac ctttcggttg
    31201 attattatcg gtatcggcgt tcgtgccatg ctggtggcct ttaatacctg gctgttgctg
    31261 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgcaggatc gctcaacggc
    31321 ctgacgtggg caaaaacctc gccttccgca cccatcatta tattgatgct cattgccgcc
    31381 gccttactgg tacgccggat gcgcttgctg gaaatgggcg atgataccgc gtgtgcgctg
    31441 ggcgtcagcg tcgaacgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    31501 gcggcaaccg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    31561 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggggcgctg
    31621 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    31681 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    31741 cgcaaaaaat gaacgaatct gtagcccgtt tgcgcggcga acagttaacc ctcggatatg
    31801 gcaaatatac cgttgcggaa aatctgactg tagaaatacc tgatggtcac ttcacggcaa
    31861 ttatcgggcc aaatggctgc ggtaaatcca ctttactgcg tactttaagc cgcctgatga
    31921 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    31981 aggttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccaggc gatatcaccg
    32041 tgcaggagct ggtggcgcgt gggcgttacc cgcatcaacc gctatttacc cgctggcgca
    32101 aagaagatga agaagcggta acgaaagcga tgcaggccac gggaataact catctggcag
    32161 atcaaagcgt ggataccctt tctggcggac aacgccagcg cgcgtggatc gcgatggtgc
    32221 tggcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    32281 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    32341 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    32401 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    32461 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    32521 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    32581 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    32641 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    32701 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgatat
    32761 ttgcttttgt taactgctca acaagatact gacgattacg taattcaccg ttcagttccg
    32821 caacgtcagt gaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    32881 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    32941 ttcccgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    33001 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgattt
    33061 ccagtttatt acggacgttt tctagtgact ctttcaccac caacgcagag atataatcga
    33121 tatacccgct caaaacggtc tgcgcctctt cactggttgg cgcggtaaaa cttagcgtcc
    33181 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    33241 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    33301 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaaccgact
    33361 gaaacttctt gataaacagg ttaaatgctt ctgtacgatc aattttgata tccagatcca
    33421 gcactcgaag cttagtgaat gttttctcca gctcttgcca ctgaacaggt tctggaggcg
    33481 tgacaaccgc cgcgctggtc catttttgcg gcaggatgaa agagatcagc aagcctgcgc
    33541 aggcaaacgc aaaaatgacc gccatgaccg tttttttggc ccgccataaa actgcgatta
    33601 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca ggaaaatgag
    33661 cgtcacttcc ctgtttaata ttcagtgatg acatggggta taacctgaaa atgagtccaa
    33721 taggggaaat ctccacggcg ctcactttac cagttggtga taattatccg aagctgaagt
    33781 ttgtaaattc cttccactga atgcaacatt cactctattt aagagaa
//
LOCUS       DAZFKM010000039        33461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFKM010000039 DAZFKM010000000
VERSION     DAZFKM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..203)
                     /locus_tag="ACHKAY_002000"
     CDS             complement(<1..203)
                     /locus_tag="ACHKAY_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO5700681.1"
                     /translation="MSYFFDTPVNIEGNKKLRSPQIEAYIKIKEFFSDEENKEALVVL
                     PTGTGKSGLISIAPFGVARKKVL"
     gene            complement(223..1392)
                     /locus_tag="ACHKAY_002001"
     CDS             complement(223..1392)
                     /locus_tag="ACHKAY_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5700682.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKAQKLAQKLSTKNSFES
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKPLELLEVLKRIEKRGA
                     LEKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFVRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWQELDLEKGLWEIPAERMKMRRPHIVP
                     LSRQVIRLFQQLKPITGHYPYIFIGRNNRKKPISKESINQVIELLGFKGRITGHGFRH
                     TMSTILHEAGFNSAWIEMQLAHVDKNSIRGVYNHAQYLNGRSRMLQFYADKLYPN"
     gene            complement(1555..1649)
                     /locus_tag="ACHKAY_002002"
     tRNA            complement(1555..1649)
                     /locus_tag="ACHKAY_002002"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1613..1615),aa:Sec,seq:tca)
     gene            1942..3324
                     /locus_tag="ACHKAY_002003"
     CDS             1942..3324
                     /locus_tag="ACHKAY_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO5700683.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQSSITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3334..5652
                     /gene="yicI"
                     /locus_tag="ACHKAY_002004"
     CDS             3334..5652
                     /gene="yicI"
                     /locus_tag="ACHKAY_002004"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO5700684.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5705..7414)
                     /locus_tag="ACHKAY_002005"
     CDS             complement(5705..7414)
                     /locus_tag="ACHKAY_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5700685.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(7535..8926)
                     /gene="xanP"
                     /locus_tag="ACHKAY_002006"
     CDS             complement(7535..8926)
                     /gene="xanP"
                     /locus_tag="ACHKAY_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO5700686.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9206..10411
                     /gene="gltS"
                     /locus_tag="ACHKAY_002007"
     CDS             9206..10411
                     /gene="gltS"
                     /locus_tag="ACHKAY_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO5700687.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10414..11280
                     /locus_tag="ACHKAY_002008"
     CDS             10414..11280
                     /locus_tag="ACHKAY_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700688.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11265..13346)
                     /gene="recG"
                     /locus_tag="ACHKAY_002009"
     CDS             complement(11265..13346)
                     /gene="recG"
                     /locus_tag="ACHKAY_002009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO5700689.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13352..14041)
                     /gene="trmH"
                     /locus_tag="ACHKAY_002010"
     CDS             complement(13352..14041)
                     /gene="trmH"
                     /locus_tag="ACHKAY_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO5700690.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(14048..16156)
                     /gene="spoT"
                     /locus_tag="ACHKAY_002011"
     CDS             complement(14048..16156)
                     /gene="spoT"
                     /locus_tag="ACHKAY_002011"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO5700691.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16175..16450)
                     /gene="rpoZ"
                     /locus_tag="ACHKAY_002012"
     CDS             complement(16175..16450)
                     /gene="rpoZ"
                     /locus_tag="ACHKAY_002012"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO5700692.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16505..17128)
                     /gene="gmk"
                     /locus_tag="ACHKAY_002013"
     CDS             complement(16505..17128)
                     /gene="gmk"
                     /locus_tag="ACHKAY_002013"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO5700693.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17386..19068
                     /gene="ligB"
                     /locus_tag="ACHKAY_002014"
     CDS             17386..19068
                     /gene="ligB"
                     /locus_tag="ACHKAY_002014"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO5700694.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19065..19682)
                     /locus_tag="ACHKAY_002015"
     CDS             complement(19065..19682)
                     /locus_tag="ACHKAY_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO5700695.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19974..20798)
                     /gene="dinD"
                     /locus_tag="ACHKAY_002016"
     CDS             complement(19974..20798)
                     /gene="dinD"
                     /locus_tag="ACHKAY_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO5700696.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(21019..21882)
                     /locus_tag="ACHKAY_002017"
     CDS             complement(21019..21882)
                     /locus_tag="ACHKAY_002017"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO5700697.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            22009..22725
                     /gene="rph"
                     /locus_tag="ACHKAY_002018"
     CDS             22009..22725
                     /gene="rph"
                     /locus_tag="ACHKAY_002018"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO5700698.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22791..23432
                     /gene="pyrE"
                     /locus_tag="ACHKAY_002019"
     CDS             22791..23432
                     /gene="pyrE"
                     /locus_tag="ACHKAY_002019"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO5700699.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23469..24065)
                     /gene="slmA"
                     /locus_tag="ACHKAY_002020"
     CDS             complement(23469..24065)
                     /gene="slmA"
                     /locus_tag="ACHKAY_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO5700700.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24172..24630)
                     /gene="dut"
                     /locus_tag="ACHKAY_002021"
     CDS             complement(24172..24630)
                     /gene="dut"
                     /locus_tag="ACHKAY_002021"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO5700701.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24608..25828)
                     /gene="coaBC"
                     /locus_tag="ACHKAY_002022"
     CDS             complement(24608..25828)
                     /gene="coaBC"
                     /locus_tag="ACHKAY_002022"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO5700702.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            26000..26668
                     /gene="radC"
                     /locus_tag="ACHKAY_002023"
     CDS             26000..26668
                     /gene="radC"
                     /locus_tag="ACHKAY_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5700703.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26885..27121
                     /gene="rpmB"
                     /locus_tag="ACHKAY_002024"
     CDS             26885..27121
                     /gene="rpmB"
                     /locus_tag="ACHKAY_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO5700704.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27142..27309
                     /gene="rpmG"
                     /locus_tag="ACHKAY_002025"
     CDS             27142..27309
                     /gene="rpmG"
                     /locus_tag="ACHKAY_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO5700705.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27407..28216
                     /gene="mutM"
                     /locus_tag="ACHKAY_002026"
     CDS             27407..28216
                     /gene="mutM"
                     /locus_tag="ACHKAY_002026"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO5700706.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28255..28734)
                     /gene="coaD"
                     /locus_tag="ACHKAY_002027"
     CDS             complement(28255..28734)
                     /gene="coaD"
                     /locus_tag="ACHKAY_002027"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO5700707.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28742..30019)
                     /gene="waaA"
                     /locus_tag="ACHKAY_002028"
     CDS             complement(28742..30019)
                     /gene="waaA"
                     /locus_tag="ACHKAY_002028"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO5700708.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30432..31490
                     /gene="rfaQ"
                     /locus_tag="ACHKAY_002029"
     CDS             30432..31490
                     /gene="rfaQ"
                     /locus_tag="ACHKAY_002029"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO5700709.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31487..32611
                     /locus_tag="ACHKAY_002030"
     CDS             31487..32611
                     /locus_tag="ACHKAY_002030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5700710.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            32604..33401
                     /gene="rfaP"
                     /locus_tag="ACHKAY_002031"
     CDS             32604..33401
                     /gene="rfaP"
                     /locus_tag="ACHKAY_002031"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO5700711.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7908 a         8267 c         8883 g         8403 t
ORIGIN      
        1 attaatactt ttttacgtgc aacaccaaat ggtgcaatag atattaaacc acttttacct
       61 gttcctgttg gtaaaactac gagagcctct ttattctctt cgtcactgaa gaattctttt
      121 atttttatat atgcctcaat ttgaggagat ctgagttttt tatttccttc aatatttact
      181 ggcgtgtcaa agaaataaga cattttattt tatccttatt tatcagtttg ggtatagttt
      241 atctgcataa aactgaagca tacgagatcg accattaaga tattgtgcat gattgtatac
      301 accgcgaatg ctgttcttat caacgtgggc aagttgcatt tcaatccatg cactattaaa
      361 accggcttcg tgtagtatgg ttgacatagt gtgtctgaaa ccatggccag tgattctccc
      421 cttaaatccc aataactcaa tcacttggtt tatgctctcc ttacttatag gcttttttcg
      481 gttatttcgt ccaatgaaaa tgtaaggata gtgaccagtg atcggtttta gttgttgaaa
      541 tagccttata acctgccttg aaagcgggac tatgtgtggc ctacgcatct tcatacgctc
      601 tgctggtatc tcccatagcc ctttttctaa atcaagctct tgccatgtag caaagcgcat
      661 ctcttgtgtt cgaacacctg taagcatcac aatcttggtt gcatttttag tgattatgct
      721 gccagtgtat gcctcaagat ctctgacaaa atgtgggagt tcctctgctg atagaaaagg
      781 atgatgtttt tgcttcggca ctgctaatgc gatagctaaa tcaggtgctg gattgtattc
      841 agcgcggcca gtgatgatcg cgtagcgaaa cacttctccg caccgttggc gtacttttct
      901 tgttttctcc aacgctccac gtttttctat gcgtttaagt acttcaagca gttctagggg
      961 tttaatttca gctattggtc gtttaccaat gaaaggaaaa acatcctgtt caaatgtttt
     1021 aataatttct tctcgataag ctaaggtcca acgatctgct tttgctgcat gccattctct
     1081 gctaacgctc tcaaaagagt ttttagtgga taatttctga gcaagtttct gagctttacg
     1141 ttcttctacc gggtcaatgc cattagcaac ctgcttacgg gcgatatcac gcttttcacg
     1201 tgcttctgcg aggcttacta aatcgtagct gccaaatgac attaaacgcg ctttccctgc
     1261 aaaccggaaa cggaaacgcc agcctttcga gccatcagga ttgataagca atgacagacc
     1321 ttgtccatcg ttcaatgtgt atggtttgtc ttgtggtttt gcacgtttga tctgtatatc
     1381 ggttagagcc atgtgtataa ctgaaaactg cgtataaaaa atctatacac attactatac
     1441 atatttttcg cggattcagg gagatgatct cggactatta cggataagct gattattcat
     1501 cgcattgaat ataaaggatt ttatggagtg cctcggaact ggctggatga ggtttggcgg
     1561 aagatcacag gagtcgaacc tgcccgggac cgctggcggc cccaactgga tttgaagtcc
     1621 agccgcctca ccggagacga cgatcttccg cgcctcgatt gctacatgga ggcggggcgc
     1681 attatagcta cttccttgag tttctacatc ccccagatca gttttttccg cctaatacac
     1741 gcagcgtatt atcacgaaag cattatagtt catataaaac ctatatttta caatgatata
     1801 tgagcgaaat taacgctacc acgatcacat aaaacaagaa tcataaatta ataaccagat
     1861 atcggaatat tcgctctcac agggatggtt tacaaaatgc gttatcggtc tcagcacccc
     1921 tatagcatca aggaaaagca gatgaagagt gaagtgttgt ccgttaaaga gaaaattggt
     1981 tatggcatgg gagacgccgc cagccacatt attttcgata acgtaatgtt atatatgatg
     2041 ttcttttata ccgatatttt tggcattcct gccggttttg tcggaaccat gtttttggtc
     2101 gctcgtgcac tggatgcgat ttccgatcct tgcatggggt tgttggccga tcgaacgcgc
     2161 tctcgctggg gtaaatttcg tccgtgggta ctgtttggcg cactgccatt cgggatcgtc
     2221 tgtgtactgg cctatagcac gccagatctc agtatgaacg gcaaaatgat ctatgcagca
     2281 attacttaca ccctacttac cttactttat accgtcgtca atatccctta ctgcgcattg
     2341 ggtggtgtaa tcaccaatga cccgactcag cgtatctcgc tgcaatcctg gcgttttgtg
     2401 ctggcgaccg cgggaggcat gctttctact gttctgatga tgccactggt taatttaatt
     2461 ggcggtgata ataaaccact cggtttccag ggcggtatcg cggtcctttc cgtggtggca
     2521 ttcatgatgc tggcattctg tttcttcacc actaaagaac gcgttgaagc accacctaca
     2581 acaacgtcta tgcgggaaga tttacgtgat atctggcaaa acgaccagtg gcggattgtc
     2641 ggtttactaa ccattttcaa tatcctggcg gtgtgcgtac gcggtggggc gatgatgtat
     2701 tacgtcacat ggattttggg cacgccggaa gtgtttgtcg cttttctcac cacttattgc
     2761 gtgggtaacc tgattggttc cgcactggca aaacctctga ccgactggaa atgtaaagtc
     2821 actatcttct ggtggacgaa cgccctgctg gcagtgatta gcctcgcgat gttctttgtt
     2881 cccatgcagt ccagcatcac tatgtttgtc ttcatcttcg tgattggtgt gttgcatcaa
     2941 ctggtgacac ctatccagtg ggtaatgatg tccgataccg tcgactacgg cgagtggtgc
     3001 aatggtaaac gcctgaccgg gatcagtttt gctggcacgc tgtttgtgct caaactgggg
     3061 ttggccttcg gcggcgctct tatcggctgg atgctggctt atggcggata tgatgcggca
     3121 gaaaaagcgc agaacagcgc cacgattagc atcattattg cgctattcac gattgttccg
     3181 gcgatctgtt atttgctgag cgcgattatc gctaaacgct actactcact cacgacgcac
     3241 aatctgaaaa ccgttatgga acagctggct cagggtaaac gccgttgcca gcaacaattc
     3301 acctctcaag aagtgcagaa ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg
     3361 attcaacctg gcctcaattt gattcacccg cttcaggtat tcgaggttga acagcagggt
     3421 aatgaaatgg tggtctatgc tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat
     3481 acgcctttat ttacgctgcg ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt
     3541 gagcattttc agggggcgct gaataacggt cctcattatc cgctcaatat tttgcaggac
     3601 gtgaaggtca caatcgaaaa cacagaacgt tatgctgagt ttaaaagtgg caacttaagc
     3661 gcgcgtgtca gcaaaggtga gttctggtca ctggattttc tgcgcaacgg cgaacgtatt
     3721 accggtagtc aggtgaaaaa taatggctac gtgcaggaca cgaataatca acgcaattat
     3781 atgtttgagc ggcttgatct tggcgttggc gaaacagttt acggtctggg agagcgcttt
     3841 actgccctgg tgcgcaatgg ccagacggta gagacctgga accgggacgg cggcacaagt
     3901 actgaacagg cgtataaaaa tatcccgttc tacatgacta accgtggtta tggggtactg
     3961 gtcaatcatc cccagtgtgt ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag
     4021 ttcagcgttg agagtgaata tctcgaatac tttgttatcg acggcccgac gccgaaagcg
     4081 gtacttgatc gttatacccg ctttactggt cgtccggcgc tgccgcccgc gtggtccttc
     4141 ggcctgtggc taaccacttc atttaccacc aactacgacg aagcgacggt aaacagcttt
     4201 atcgatggta tggcggaacg caatctgccg ctgcatgttt tccactttga ctgtttctgg
     4261 atgaaagcct tccagtggtg cgattttgag tgggacccgc tgactttccc tgacccggaa
     4321 gggatgatcc gccgcctgaa agcgaaaggg ctgaaaatct gcgtctggat taacccctat
     4381 atcggtcaaa aatcccccgt ctttaaagag ttacaagaga aaggctattt actcaaacgc
     4441 ccggacggtt cgctatggca gtgggataaa tggcagccag gtctggcgat ttatgacttt
     4501 accaatccgg atgcctgcaa atggtacgcc gacaaactga aaggtctggt cgcgatgggc
     4561 gttgattgct ttaagaccga ctttggcgaa cgtatcccaa ctgatgttca gtggtttgac
     4621 ggttccgatc cgcagaaaat gcataaccat tatgcataca tctacaacga actggtgtgg
     4681 aacgtgctca aggacaccgt tggtgaggaa gaagctgtct tgtttgcccg ctcggcctcc
     4741 gtcggtgcgc agaaattccc ggtacactgg ggtggcgatt gttacgctaa ctacgaatca
     4801 atggcggaaa gcctgcgcgg tggtttgtct attggccttt caggttttgg cttctggagc
     4861 cacgatatcg gcggctttga aaataccgct ccggcgcacg tttacaaacg ctggtgcgcg
     4921 tttggtttgc tctccagcca tagccgttta cacggtagca aatcttatcg tgtgccgtgg
     4981 gcctacgatg atgagtcctg tgatgtagtg cgcttcttca cgcaactgaa atgccgcatg
     5041 atgccgtatc tgtatcgtga agctgcgcgt gcgaacgcgc ggggtacgcc gatgatgcgg
     5101 gccatgatga tggagttccc ggacgatccg gcttgtgatt accttgaccg tcaatacatg
     5161 ttaggcaaca acgtgatggt tgcgccggtg ttcactgaag cgggcgatgt gcagttctac
     5221 ctgccggaag gtcgctggac acacctgtgg cacaacgatg aactcgacgg tagtcgctgg
     5281 cataaacagc agcacggctt cctgagtctg cccgtttatg tgcgtgataa cactctactg
     5341 gcgctgggca acaacgatca acgtcccgat tacgtgtggc acgaaggcac ggcattccac
     5401 ctctttaatc tgcaagacgg gcatgaagcc gtctgtgaag tgcccgctgc tgacggatcg
     5461 gtgatcttta ctttaaaagc agcacgtact ggcaacacga ttactgtgac tggtgcgggc
     5521 gaggcgaaga actggacact gtgcctgcgc aatgttgtga aagtaaatgg tctgcaagac
     5581 ggttcgcagg ctgaaagtga gcaggggctg gtggtgaagc ctcaagggaa tgcgctgaca
     5641 attacgttgt aatctattga tggaatgccg ggtacgtctg agtcgtaccc ggcgagattg
     5701 atgtttactg caccagctct gccgacgtaa cttcaccgct ggaaacgatg ccagcattca
     5761 tggtttgagt cacttgctgc tttgcggcgt tcacggcatg caattgcccg ctaaccgtag
     5821 gtttcagcgg ggcattggcc tgaatatctc cactggcggt aagctgaata ttgccgtcgc
     5881 cagtcaacgg taattcaggc catccccatt gttgcaaaat attcaccggc acaccgcgtc
     5941 cattcaggct gatatgggtc tgacgttgtg gcgtttgtga aacactggca gtggcttcca
     6001 gaatgccttt ttcagtaaat gcactcagtt cgctgatatt caccgtgctg ctgttggcgg
     6061 tcagcgccag cgacgggcga cgaacatcaa cacgattgaa tgtcgcggcg gcggcattca
     6121 gattcgccga gccactccag acgccccatt tatgatcggt aaccagcgtc aggttggcac
     6181 cgtaaccatc gagcgcggtg agctgccacg ggaagtcagg gtcgatatca atgatcagat
     6241 tgcggctggc gctaaatctc ttcagttgca ggctgtttaa ccaaccgggt gtcgtttcca
     6301 tccacaactg ttgccagttt ttcggcaagg tatattccag cccggcaatt gccacatcat
     6361 caaggatcaa cgttttcccg tcacgcagcc agttccctga cgttctgacc ataccccctt
     6421 cccagcggct ggtgaactgg cgcagcgcta cgccctgcgg ggaaaattcc gcgtttataa
     6481 tcgggtcaaa taaatgcagc gaaccataaa tgaactcgct agcgttcatc gacagtttgc
     6541 cttcctgtgt ctgccagtca tctttactga aggtcatgtt gcgcaagctg agatcgaggt
     6601 cggtcaccgc ccagtccgga ccttgcaaac gagcatcgat cacttccagg cgaccaatct
     6661 gcaacgacgg gacagagcgt aatggcgcaa agaagtcggt tagcgatttt tcgctttgta
     6721 gacgtatatc cgccatgcgc aggttttcca cttgccagct gccgtcggcg ttacgctgcg
     6781 cgtttccggt taatgtcccg cgggcgatgt cggcacccag gttagtcagc gtaacgcgat
     6841 cgttatcaat actgccttca atcagtacat tggtggcagg aacatcattg agcgaaagcg
     6901 atccggcact aaactgaatc tgcgccttcg tacccagcac tttaccggct tttggtgacc
     6961 acggaactac gccgccattt atccgctgcg cgctcagttt ccattcgcta ttcgggctat
     7021 taaacgccat atcacgcagt tgcagacgat cggctttgaa cggtagcggc gcggtctggt
     7081 cggtgagatt cagcgtcccg ttttccagca ggatggtatc gacatggcgt ggttcggtca
     7141 gttgccgact gcttagcgca atgtcgacac tttttgccac cagggtcgcg ggctggccat
     7201 cacggccaaa cgtgacgttc tccagcacga tatgagatgg cgcggaaaaa cggtgatcca
     7261 tcgccccgaa ggccagatga tagtcgctat tctcggaaac ccatgcgctg atatgttctg
     7321 ctccccagcg ggtttgcaga agaaaataga ggccagcgat caccactaac agagcgatga
     7381 gaatgtagag aagcagcttc ccaataaatt tcatggtctt ccatcccgtg aaatgcacat
     7441 aagggagtta tgcacgattt acgcgcaatc ctcaaggcgg gaatggtgaa agaggtgaga
     7501 ctgcggcggt aattatctta ccgccgccag tgaattactg tttttctggt gggaaaatca
     7561 gattcagcac gatggcagta ataccgcccg cggcgatccc ggaggagagc aggtttttca
     7621 gccattcagg ggcaaactgc aaaatcagcg gctgctgaga cacgcccaga ccaaccgcca
     7681 gcgacagcgc gataatcaga atcgcccgac ggttcagcgg ttcacgagaa acgatacgca
     7741 caccggaggc ggcgatagtg ccaaacatta caagcgttgc gccgcccaga accggttctg
     7801 gaatgtgttg tacaaaaccg ctcactgccg ggaacagacc cagcacgatc aacatcagcg
     7861 cgacgacaaa accgacatag cggctggcaa caccagtcaa ctggatcact ccgttgtttt
     7921 gcccgaagca ggagttcggg aaggtgttaa acaccgccga aacaaacgag ttcaggccgt
     7981 ttgccagcac gccgcctttc aggcgtttca tgtacagcgg accggacact ggctgttcgg
     8041 aaacgtcaga ggtcgccgtg atatcgccaa tggtttccag cgaagtgatc ataaagacca
     8101 gcatcagcgg cagcagcaga ctccattcaa tgccaagacc gtaatagagc ggcgttggca
     8161 ccataatcag ttcttgcgtc atcggttcgt tgctttctgg caacatgccc ataaaccacg
     8221 ccagcgcata tccggccgcc atcgcaatca ccagtgaggc cacgcgtaag taagggttac
     8281 gttgacggtt aagcaggata attaaggcta agaccacgcc tgccagcagc agatttttcg
     8341 gtgcgccgaa ggtgttatcg ctcatggctg cgtaaccgcc gccaatggac gttaacccaa
     8401 cctgaattag cgacaggccg ataatcatca ccacaacgcc agaaaccagc ggcgtaataa
     8461 tgcggcgcgc cagatgcaga acgcgggaga tcaccatctc ggtgcaactt gccagcatca
     8521 acgtgccgaa caaagccgcc atcatggtag gaacatcagc accaccggtt ttcagcgcgg
     8581 taccgcccat aatcagcggg gcaacaaagt tgaagctggt gccctgaata gacaacagcc
     8641 cggagccaac cggaccccag gccttaattt gaataatcga tgccacaccg gaggcaaaca
     8701 gcgacatact aataatgtgt tgcgtgtctt gtgccggtaa acccagcgcc tggcagatta
     8761 atagcgctgg cgtgatcacc gcaacgaaca tcgccagcag atgctgacag gcggcaaaca
     8821 gggtttgagg aagcggcgga cgatcttcaa gacggtaaat cagttcgctg ttttgagtct
     8881 gcgcaaccgg ttgcgcattt tctgactcga gggtggaaac agacatcgaa aacaatcccg
     8941 tggtagcaaa gcgggcattt tagctgaccg aaagagaaaa gcaaacgttt gccacatcct
     9001 catctaaata tttaaatgag ataaatgcac ttttttatat aacttttggt atttttctgc
     9061 ttaaaatcca tgccatttgt tcattaacag ctgagaattt gacccaggaa agcataactc
     9121 gatacccgtc atagttcatg aagttgccac tgcgcgatga agtatgacga gtatgaaaga
     9181 gtgatgcgga tacaaaggag taactatgtt tcatctcgat actttagcaa cgcttgttgc
     9241 cgcaacgctg acgttgctgc tcgggcgtaa gttggtccat tccgtctcct ttttgaagaa
     9301 atacaccata ccggaacctg ttgcgggtgg tttgttggtg gcgctggcgc tactagtact
     9361 gaaaaaaagc atgggctggg aagtcaactt tgatatgtcc ctgcgcgatc cgttaatgct
     9421 ggctttcttc gccaccattg gcctgaacgc caacattgcc agtttgcgtg ccggtgggcg
     9481 tgtggttggc atcttcttga ttgtggttgt tggtctgttg gtgatgcaaa atgccattgg
     9541 cattggtatg gctagcctgt tagggcttga tccgctgatg gggctgttgg ccggttctat
     9601 tacgctttcc ggcggtcacg gtacgggcgc tgcgtggagt aaattgttca ttgaacgtta
     9661 tggcttcacc aatgcgacgg aagtggcgat ggcctgtgca acgttcggtc tggtgctggg
     9721 cggcttgatt ggcggtccgg tggcgcgcta tctggtgaaa cactccacca cgccgaacgg
     9781 tattccggat gaccaggaag tcccgacggc gtttgaaaag ccggatgtgg gacgcatgat
     9841 cacctcgctg gtgctgattg aaactatcgc gctgattgct atctgcctga cggtggggaa
     9901 aattgttgcg caacttttgg ctggcactgc ttttgaactg ccgaccttcg tctgtgtact
     9961 gtttgttggc gtgattctga gcaacggtct gtcaatgatg ggcttttacc gcgtctttga
    10021 gcgtgcggta tccgtgctgg gtaacgtaag cctgtcgttg ttcctggcga tggcgctgat
    10081 ggggctgaaa ctgtgggagc tggcttcgct ggcgctgccg atgctggcga ttctggtggt
    10141 acagaccatc ttcatggcgt tgtatgccat cttcgttacc tggcgcatga tgggcaaaaa
    10201 ctacgatgcg gcagtgctgg ctgcgggtca ctgtggtttt ggcctcggtg caacgccaac
    10261 ggcaatcgcc aacatgcagg cgatcactga acgctttggc ccgtcgcaca tggcgttttt
    10321 ggtggtgccg atggtcggtg cgttctttat cgatatcgtc aatgcgctgg tgattaagct
    10381 gtatttaatg ttgccgattt ttgccggtta aaaatgaaaa agcgtcggaa ggaaccggaa
    10441 acgctacgtg aacactgtcg ccatattttt ggtgatgagc cgccagtgct ctgtgtatgg
    10501 gaaacggaat ttgattacgc agatgccgaa ctcaaggcgc tggcggctaa agagtggcag
    10561 cagatcagcg aatgggattt gagtgcttac tatgtactca atctcgttta taacgaaccg
    10621 atgcaaatag agttgtttcg ctatctgttt ccgctctgcc tggcgcaatg gcatgaaacc
    10681 gttttagctg gcggttatgg tgatcacttc gaagaatcct taatgaaggc attgtgtcgg
    10741 ccctatctct ggcaggagat gatgaatgct tcccaacgcc agcaggtacg gcaattttta
    10801 ctggacaccg cgttgcagcg tatggataac gagcgtggtt tcaataacgt tctttgctgg
    10861 ctggcggtgt tcaatactct gggtggggcc gcgccgttaa ttcgttctct ctggtcgcgc
    10921 tggtgggcgc tggatacgcc aggaaaagcg gtgtgtgcta ttcagtatgc cgcgcatcta
    10981 atttatccca tagaagccaa tccgttatgg tcgcaagaat ggataggctg ggggcatcca
    11041 ttaggccata aagatggctg gtcctctgat aatcgcgctt ttcttcgcca aatgttaaca
    11101 cctgaaatga ttgtggctgg cgtacaggca gcggctgaaa ttctgcgtgg tgaaccggaa
    11161 ggcgcaatgg cggcgcgaat tgcacaggat gcgtatgagg caatggatat cctcactatc
    11221 cagattgaag atttactgcg tgacttgtct tgtgatgagt ctggtcatgc gttggagtaa
    11281 cgttccgtct ccggcatcca gcgttctatc agggcttttg cttgttgtgg gtaacgttcg
    11341 tgaatatgtc gcgccaggcg ctgaacttcc gggatcatcg cctgatcgcg cagtaaatcc
    11401 gccactttaa actcagcatt acccgtctga cgcgtaccta acagttcacc cggtccgcga
    11461 atctctaagt ctttctgtgc aatcacaaaa ccgtcgttgc tgtcgcgcag cacttgcagc
    11521 cgaatttgcg cggttttgga gagcggcgtt ttgtagagca gcacgcagtg agaagccacc
    11581 gcaccacgac ctacacgccc gcgaagctgg tgtaactgcg ccagacccag acgctccggg
    11641 ttttcgataa tcatcagact ggcgttaggc acatcaacgc cgacttcaat aacggttgtg
    11701 gcaaccagca ggtgtagctc accttgttta aacgacgcca tcaccgcctg tttctcggca
    11761 ggtttcatcc gcccgtgtac caggccaacg ttcaactctg gtagcgccag tttcaactct
    11821 tcccaggtag cttccgccgc ctgcgcttcc agcaattccg actcttcaat caacgtacaa
    11881 acccagtatg cctgacgacc ttcagttatg caggcgtggt gcacgcggtc aatgatgtcg
    11941 gtacggcggg tatcaggaat agcgaccgta gtcactggcg tgcggcctgg cggcagctca
    12001 tctatcaccg aggtatcgag atcggcatac gcagtcattg ccagcgtgcg ggggatcggc
    12061 gtggcggtca tgatcaactg atgcggatgg aagccctgct gctggccttt ctcccacaat
    12121 gccagacgct gatgcacgcc aaaacgatgc tgttcgtcga taatcaccag cgccaggccg
    12181 ttaaactgca cctgttcctg gaagatggcg tgtgtaccga caatcatctg cacctgaccg
    12241 ctggcgatgg cttcctgctg tgccagccgt gctttacctt tctgcttacc ggcgagccag
    12301 cccacttcga taccgagcgg tgcaaaccag ttgcggaagt tattggcgtg ctgctcggcg
    12361 agtaattcgg ttggtgccat caatgctacc tgtttgccgt gggcaatcgc acgcaacgcg
    12421 gcgagggcgg cgaccagcgt tttaccggaa cctacatcgc cctgcaccag acgcatcatc
    12481 ggcacatcca gcgccatatc gcgctcgatc tccgccacta cgcgtgcctg tgcgcccgtt
    12541 ggcttgaacg gtaaggcggc gaggagttta tttttcagcg tgtcattggc gctcagcggc
    12601 tgggcatgaa aacgctgtgc tccggcacgt aaggctaaca tgctgaggtt gtgcgccagc
    12661 agttcttcca gaatcagacg acgttgcgcc ggatgctgcc cggtttccag atcgctaagc
    12721 tgtagcgtcg gtggcgggcg gtgcaaagtg cgcaacgctt ccggtagcgt catcattcct
    12781 tgtgacagtt ccggcggcag gagttcttca atggcgcagg tgtcgagcag atccagcgcc
    12841 tggtcggtta atttacgcag cgtggcctgc tttacgcctt ccgttgttgg ataaaccggc
    12901 gtgagcgttt cctgtaattc tggcgtgctg agatcgccct gcacgcggta ttccgggtgg
    12961 atcatctccg caccatattt accgcgcttt gcttcgccat aagccagtac acggcggccc
    13021 gccgccaggc tatttttcat tgccgcgctg aaattgaaaa agcgcatggt gaggatgccg
    13081 gaaccgtcgc tgatctggca ggtcatcatc cgccgaccgc cgaaggagat attgcagttc
    13141 agcacttcgc cttccaccgt ggcataaacg cccggcagta gttctccgat ggggtagaga
    13201 tgggtgcgat cttcgtagcg caggggaagg tgtaagagta gatcctgcac ggtatgcagg
    13261 ttgattttcg ccagtttgtt actaagtgct gcgccaacgc ccgttaggga actgagtggg
    13321 acagcatcta acaggcgacc tttcatggca cttaccctgc agcctgcata gtagcccacc
    13381 agtcggcatc agcttcgatc tcgccttgct gattgacgtg gggataaggc aggcctttgc
    13441 gttttgcgac tttcgccagc accggatagc cgccttcaaa caacaggcgt tgttgctctg
    13501 cttccggcaa catgctgttt tcacgcaggt acatgcctgc attttgccgc tgacgctggg
    13561 cttcgtaaag aatgagggct gaggcaacgg acacattcag cgactgcacc atgccgatca
    13621 tcggaatgat gatgtcctga tccgccaggg ccaatgcttc ctgcgtgatg cccgttttct
    13681 cctgtcccat caaaatgcag gtcgggcgag tgtaatcaat ttcgcggaaa tcgacagcgt
    13741 tatcagaaag atgggttgcc agaatctgca tgccctggcc tttgagatga gcgacggcat
    13801 cgccaatggt gcggtgtgtt ttcacctgta cccagctgtt actacccgcc gctgccgaag
    13861 ccatggtgcg catgcggcta ccaggccaga cggcgtgaac ttcatgtacg ccaacggcat
    13921 ctgcggtacg aataatcgca gaaacgttat gaggtttgtg gacctgctcc atgcagacgg
    13981 tcagatcagg ctgccgcctg gcgagcattt cgcagatgcg tgcataacgt gttgggttca
    14041 taaaacatta atttcggttt cgggtgactt taatcacgtc tggcatcacg cggattttgc
    14101 gcatgatatt cgccagatgc acacggtcac gagcggtcag acgaataaag gcgctgtaga
    14161 cgcgaccatc tttctcttcc gtattcaaac tttgaatatt cgaggtcgtg gtgttaattg
    14221 ccgccgtcag gtttgccagc gcaccctgat gattgaacat ctccaccttg atttcggtga
    14281 tgaactcctg cgccgtctct ttatcccatt ccacagccat aaacttctct ggctctttct
    14341 ggtagccacg gatgttacgg caggattcat ggtggatcac cagaccttta ccggggctga
    14401 cgtgggcgat aatcgggtcg ccaggaatgg ggcggcagca tttcgcaaag gtgatcagca
    14461 cgccatcagc gcctttgatc ggcagatgcc cgtggctttg agtcgccggt ggaatggagg
    14521 cgtccccatg ttgtagattt ttcgcgacca ccacgctcat tgcgttacca aggccgattt
    14581 ctgccagcag atcgtcaagc gttgccagct tcatgcgatc cagctcgcgc tgaatatttt
    14641 cctgcgggat ttcattgagc ttacggctac cacccaaagc atggttgagc agacgacggc
    14701 ccaggcttac agaatcatca cgcttgaggt ttttcagcaa ctgacgaatt ttggcgcgcg
    14761 ctttcgagct aacgacaaag ttcagccaag cggcattcgg gcgagcgccc ggagcggtaa
    14821 tgatttcaac ggtctgaccg ctagtaagcg gctgcgacag cgggtaaggc tggcggtcga
    14881 cgcgtgcgcc cacgcaggca tggccgatat cggtatgcac tgcataagcg aagtcgacgg
    14941 gcgttgcacc ggcaggcagc tcgacaatgc gcccttccgg tgtgaaaacg taaatctcat
    15001 ccgggaagag atcggattta acgctctcga taaattcaaa cgaactaccg gcgctctgtt
    15061 gcagctccag caggctttgc atccagcgct gggcgcggat ttgcgcggtg gtgctggttt
    15121 cgccgtgctc tttataagcc cagtgcgcgg caacacccat ctccgccatc tgatccatat
    15181 cttcggtacg gatctggacc tcaaccggca caccgtgcgg gccgatcatc gaggtgtgca
    15241 aagactgata gccgttcgct tttggaatgg cgatgtagtc tttcacgcga cccggacgcg
    15301 gcttgtacag gctatgcatt tgcccgagca cgcgataaca ggtgtcggaa tcattgacga
    15361 tcacgcggaa agcgtagatg tccatgattg agtgaaaacg ctgctctttg agcaccattt
    15421 tgcagtaaat cgaataaaga tgcttctcgc gaccactgac gcggcacgga attcccgctt
    15481 cctgcaaacg cccttcgatt tcagagagga ttttttggat catctcttta cggttgccgc
    15541 gcgcggcttt caccacttct ttaattacgc ggtaacgatt gggatacagc gcctcaaaac
    15601 ccagctcttc gagttcggtt ttaatgtggt ggatacctaa acggtgcgcc agcgggctgt
    15661 aaatttcgag agtttcacgg gcgatgcggc gacgtttgtc cgggcgaagt gagcccagcg
    15721 tgcgcatgtt gtgggtacgg tcggcaagtt tgatgaggat gacgcggata tcctgcacca
    15781 ttgccataat catcttgcga aagttttcgg cctgcgcctc tttcttatca cggaacttga
    15841 gtttatcaag tttcgacacc ccctctacca gctcggcgac gcttttacca aaaagctgtt
    15901 ccatatcctg gtaggtggcg ggagtatctt caatcacgtc atgcagcagc gccgccatca
    15961 gcgtttcata gtcgagtttc atctcggcca gaatgcaggc aaccgctacc gggtgcgtga
    16021 tatagggttc accgcttgaa cgtgtttgcc cctcgtgagc atcacgtgca acgagatacg
    16081 cctgccgcag acgcttgatt tggtcttccg gcaggtaggt ttgaatcagt tgattcaggc
    16141 tttcaaacag atacaagggc gacccgcttt gtgattaacg acgaccttca gcaatagcgg
    16201 taacggcttg taattcagcg gcttcctgct cttgctgttc ctggcgttcg cgaacgtcga
    16261 ggatctggtt gttgatcaga ccttcttcga tttcgcgcag cgcgattaca gtggttttat
    16321 cgttttcttc cggtaccagc ggatcctttc cgcctacctg catctgacga gcgcgacgcg
    16381 cggcgaccag taccaggtca aaacggttac caattttctc tacagcgtcc tgaacagtta
    16441 cgcgtgccat acttaaaaag ctccacaggt gaagaaatga ctgggcatga tactgaaatc
    16501 aggttcagtc tgccaacaat ttgctgatta aagcgtcatg acgctgcttt tggcggctca
    16561 tgcgcagacg ttcggcgcga ataatggtct tcaaatcggt caacgcggta tcgaagtcat
    16621 cattcacaat cagataatca tattcggcgt aatggctcat ttctgcaaca gcttgcgcca
    16681 tacgctttgc aatgacctct tcgctgtcct gaccgcgacc gcgtagacgg cggtccagtt
    16741 caattttgga cggcggtaaa ataaagatac tccgcgcgtg cggcatcttc tggcgaattt
    16801 gctgcgcgcc ctgccagtcg atatcgagaa aaacatcgac accggtcgcc agtacttgct
    16861 caatggcctc acgcgaagtg ccatagtaat taccaaaaac ttctgcgtgt tcgaggaacg
    16921 catctctgct aatcatttct ttaaattcat catgattaac aaagaaataa tgttcaccgt
    16981 ggacttcacc aggacgcggt tggcgtgtgg tgtgtgaaac agaaacctgg gtgtcataca
    17041 acggttgggt ttttaataaa gcctgaatca ggctggattt acccgcgcca ctgggggcag
    17101 aaacaatata aagcgtgcct tgagccatga gtatctttcg tatgtgatta gcgaaataaa
    17161 gcctacatac gagcttatta tacacggcgc ggccatgtga cgtagccctt gctcacactt
    17221 tttgcgcaaa gttgttgcgt tttgcgtagc tgtttccgca tttgcctgtg atttactgca
    17281 ataaagacga ttctcaggaa gcgtcctcgc agactccgtt tttgccactc ggctttgcct
    17341 tcaccacgtt gtatatctgc cagcctgact acagcggagg tgaagatgaa agtatggatg
    17401 gcgatattaa taagtatctt gtgctggcaa tcatctgtgt gggcggtctg tccggcctgg
    17461 tcgccagcca gagcacagga agaaatttcc cgcctgcaac agcaaataaa acagtgggac
    17521 gatgactact ggaaggaagg aaaaagtgag gtggaagacg gtgtttacga tcagttaagc
    17581 gcccgtctta cgcagtggca acgctgcttt gtgagcgagc cccgcgacgt gatgatgccg
    17641 cctttaaatg gtgcggttat gcatccggtt gcccatacgg gtgtacgcaa aatggcggat
    17701 aaaaacgcat taagtctgtg gatgcgagaa cgtagcgatc tttgggtgca gccaaaagtt
    17761 gatggcgttg cggtaaccct ggtttatcgg gacgggaaac tgaacaaagc aatcagtcgc
    17821 ggtaatggcc tgaaaggcga ggactggacg cagaaagtta gcttaatttc cgctgtgccg
    17881 caaaccgtta gcgggccttt agccaacagt acgcttcagg gggaaatatt tctccagcgc
    17941 gaggggcata tccaacaaca aatgggggga ataaatgccc gcgcaaaagt tgctggcctg
    18001 atgatgcgcc aggacgatag cgacacgctg aattctctgg gcgtttttgt ctgggcatgg
    18061 ccggatggac cgcagttaat gaccgatcgt ttaaaggagc tggctaccgc aggttttact
    18121 ctaacgcaga ggtatacccg tgcggttaaa aatgctgatg aggttgcgcg cgtacgcaat
    18181 gagtggtgga aagcgaaatt acccttcgtc accgatggcg tggttgtgcg aggggcgaaa
    18241 gaacccgaat cccgccattg gctaccaggc caggcagaat ggctggtggc ctggaaatat
    18301 caacctgtag ctcaggttgc cgaagtgaag gcgattcagt ttgcggtggg taagagcggt
    18361 aaaatatcgg tggttgcgtc actcgcacct gtcatgctgg atgataaaaa agtccagcgg
    18421 gtgaatattg gttccgtcag gcgctggcag gagtgggata ttgcgcctgg tgatcagatc
    18481 ctcgtcagcc ttgccggtca gggtattcct cgcattgatg atgtggtctg gcgcggtgca
    18541 gaacgtacaa aaccgacacc accagaaaac cgctttaact cgttgacctg ctactttgct
    18601 tctgatgttt gtcaggaaca gttcatttca cgcttagtct ggctggggtc aaaacaggtt
    18661 ctggggctgg atggtattgg tgaggccggc tggcgcgcgc tgcatcagac tcatcgcttt
    18721 gagcatatct tttcctggct tttattaacg ccagagcaat tacagaacac gccggggatc
    18781 gcgaaaagta aaagtgcgca gctatggcat cggtttaatc tggctcgtaa gcagcctttt
    18841 actcgctggg tgatggcaat gggaataccg ctaacccggg cggcgcttaa tgccagtgat
    18901 gaacggtcct ggtcgcaact tttatttagc acggagcagt tctggcagca gctgccgggg
    18961 actggatcgg ggcgcgccag acaggttatt gaatggaagg aaaatgcgca aatcaagaag
    19021 ctgggcagtt ggctggctgc ccagcagatc acaggttttg aaccttagtg tccttcgtgg
    19081 ctgtagtaaa agaccggtaa tcccagctta aggcgtagcg ccagtaagcg ggcaaaaaag
    19141 ccgaatacca gggtggagat aattaccaca tcatggttag aaacatagtg ttgcagcgcg
    19201 atgtacagca cggcggaggc aaatgagaca ccggcataca actctttctg gaataccagt
    19261 gggatacgtt tacagaacat atcgcgcaaa acgccgccaa acacgccggt ggttaccgcc
    19321 gcgacaacgg caataattgg gccgtgcccc atatccagtg caacctgtgc gccgatgata
    19381 gaaaagacca ccagtccaag cgcatcgagc accagaaaca ctttgcgcag gtaaggcatt
    19441 acaggagcaa cgatagtggt gagtactgcg gcggtagcga cgataatcac atattccggg
    19501 tgtttgaccc agccgagcgg atagtggccc agcagaatat cgcgcactga ccctccgcca
    19561 attgcggtgg cggtcgcaat aataattacg ccaaatgtat ccatgcggcg tcgcccggcc
    19621 gccagagccc ccgtcatggc ttcggcagta atgccaacca aatacaaaat gtgtaacagc
    19681 atggttctct ccctaaaaat ctgtctggca gggtagcgat ttgtgcgtat tgtcacgatt
    19741 gagatttttt aaggtgagag gaaattgata acttgtagtt atgtgaaatc gctattttct
    19801 gtagcgtaga tgcattcttc tgacttcttg tctgagaaaa tgcataagaa aaaataggtt
    19861 aaaattgagg ggtggaaaag aaaggtgcaa tgtttagcca ttgtgttcac cgcacctgga
    19921 ctttggctta cgcatcgggt atctcaatca acaaaaagag tttttagttt ctgctatttc
    19981 ttctctgtaa tttttacact gttttccaac tgcttgatgc tgacctgcgg ggtcggcaac
    20041 tcctcaggca tggttccgcc aagttcctga atggtttgcc tcaccttgct acccacgtca
    20101 aagtgtgtgg tattagcttg ctgttttgaa ttcacgccat cccgcttgag tttttcttct
    20161 gtttgggtag ctcgaaagag attagccgcc agttctgttg aacccatatg atccaggatc
    20221 ttctgactct ttttcagccc cttccgctga tggatggctt tctgatctaa tccaccatac
    20281 agcccccggt aaccatgatt ctgaaatatg gcgaaatcga tagctgtcgc taccccagct
    20341 tgctgcgctg cctcaaccaa ttgtttgtta tgttctttca attcatttct cagaaaaaga
    20401 cgcttttcgt cttcacgaag ttgcctgaaa gcttcatcgt cagccagctc ctgccgtcgg
    20461 gtctggatag caaaataagt ttgccctgcc gcaataaccg gtttcgcagg gtcgccgttt
    20521 tgtactacca ggtaacaggc atagcgggag agatgaacat cttcaagttc tcgttgagcc
    20581 cctgaaccta atacaaccat tttggtggtt tcaacgaaat ggtcagatgc agcttgattg
    20641 ctggcttcgc acgcttgagt cgcgcgggcc agtacctttt gaaaattgcg ccagtcccga
    20701 taatccagta acggtgccaa ttctctagct gaccaaaatt cttgcccttc agtgccataa
    20761 tgccgtattt cttcaaaagg ttgatgatgt tcgttcattg cccactccat gtgatatcga
    20821 ttgcaaagaa agtaaatctg taactgtatt tatatacagt tgtgagtatc tcacgttctc
    20881 agtgtgctgg gcaaaaaaac atcgcaaaat ctggagcagg cacgcaaaac agaattgaag
    20941 ttgcggggaa gaaaaatgcc gctctgtttg agtgggcggc atttgtgaag gctgaaaagt
    21001 aagaattagt gttacgagtt actcaatatt ctgaatctgc tcgcgcatct gctcaatcaa
    21061 cactttcagc tcaatcgcgg agtttgtcac ttcggcattg atagacttcg acgcaagagt
    21121 gttcgactcg cggttgaatt cctgcatcat aaagtccaga cgacgaccaa cggcttcttt
    21181 tttcttcaga atgttgtagg tctctttgac atgcgcttcc aggcggtcca gttcttcggc
    21241 aacgtcaatt cgttgtgcca gcagaaccaa ctcctgctcc aggcggttgt tttccagttg
    21301 cacttgagca tcttccagct tcgcgaccag acgctcacgc tgccattgca ggatttccgg
    21361 catatgggcg cggactttga ccacttcggc ggtgacgcct tccagacgct gctcgatcaa
    21421 tgctttcagc gcctgacctt cggtttcacg tgcgacgata aagtcgtcca gggtaccatc
    21481 caacgccgcg agaatttcag cggcaatcgc gtcgagatcc tgctcctggg ctgccattac
    21541 gcccggccag cgcagaatat caaccgggtt gatttcccct tcgtcactct gcatttttac
    21601 ccagttcgcg gcagttacca gctgtttagc cagtttttcg ttgaggatca gctcaccttg
    21661 cgcgctaaca tctggctcat agcgcagggt acattccact ttaccgcgcg tcaggcgaga
    21721 acgaatacgc tcgcgaacga caggttcaag gctacggaac tgctccggca gacgaaagta
    21781 agtttccaga taacgctggt ttaccgagcg catttcccag gttgcgctcc cccattcacc
    21841 cttgatttca cgccgggcgt aggcggtcat actgcggatc atagacgttc ctgtttataa
    21901 aaggagaggt ggaaggatta tagccatcga tgccttgtca ggataggaat aaccgccgga
    21961 agtccgtata atgcgcagcc acatttgttt caagccggag atttcaatat gcgtccagca
    22021 ggccgtagca ataatcaggt gcgtcccgtt accctgactc gtaactatac aaaacatgca
    22081 gaaggctcgg tgctggtcga atttggcgat accaaagtgt tgtgtaccgc ctctattgaa
    22141 gaaggcgtgc cgcgcttcct gaaaggtcag ggccagggct ggatcaccgc agagtacggc
    22201 atgctgccac gttctaccca cacccgtaac gctcgtgaag cggcgaaagg taagcagggt
    22261 ggacgcacaa tggaaatcca gcgtctgatc gcccgtgctc ttcgcgcggc agtagatttg
    22321 aaagcgctgg gtgagttcac cattacgctg gactgcgacg tgcttcaggc tgatggtggc
    22381 acgcgtaccg cgtcgattac gggtgcctgc gtggcgctgg cagatgcgct acagaagctg
    22441 gtggaaaacg gcaagctgaa aaccaacccg atgaaaggga tggtagccgc agtttctgtc
    22501 ggtattgtaa acggtgaagc aatttgcgat ctggaatacg ttgaagactc agccgcagag
    22561 accgacatga acgtagtgat gaccgaagac gggcgcatca ttgaagtgca ggggacggca
    22621 gaaggcgagc cgttcaccca tgaagagcta ctcaccttgt tggcgctggc ccgaggggga
    22681 atcgaatcca ttgtagcgac gcagaaggcg gcgctggcaa actgattttt aaggcgactg
    22741 atgagtcgcc ttttttttgt ctgtagaaaa gtaagatgag gagcgaaggc atgaaaccat
    22801 atcagcgcca gtttattgaa tttgcgcttg gcaagcaggt gttaaagttt ggcgagttta
    22861 cgttgaagtc cgggcgcaaa agcccctatt tcttcaacgc cgggctgttt aataccgggc
    22921 gcgatctggc actgttaggc cgtttttacg ctgaagcgtt ggtggattcc ggccttgagt
    22981 tcgatctgct gtttggccct gcttacaaag ggatcccgat tgccaccact accgccgtgg
    23041 cgctggcgga gcatcatgac cttgacctgc cgtactgctt taaccgcaaa gaggcaaaag
    23101 accacggtga aggcggcaat ctggttggta gcgcgttaca aggacgcgta atgctggtag
    23161 atgatgtgat caccgccgga acggcgattc gcgaatcgat ggagattatt caggctaatg
    23221 gcgcgacgct tgctggcgtg ttgatttcgc ttgatcgtca ggaacggggg cgcggcgaga
    23281 tttcggcgat tcaggaagtt gagcgtgatt acaactgcaa agtgatctct atcatcaccc
    23341 tgaaagacct gattgcttac ctggaagaga agccggaaat ggcggaacat ctggcggcgg
    23401 ttaaggccta tcgcgaagag tttggcgttt aaagaaactc gccggatgaa aagtcatccg
    23461 gcgtcatatt actgcaactg tgccgcaatt agcggccagc gggcgtcaaa atcatccgtc
    23521 gggcggtatt taaattcgct gcggacaaaa cgtgacagca taccttcaca gaaggccagg
    23581 atctggcttg ccagcagggt ttcatcggtg gtgtaacctt caccctcacg cattctcttt
    23641 tcacgcaata cctggcgcag ctgcgcttca atacgctcga acagctggtt gatgcgccct
    23701 tgcaggcgat cctgttcaaa cattagcgca tgaccagtga ggatgcgggt caggccagga
    23761 ttacgctcac caaaaccgag aagcagcaac acaatcagac gcaggcgcgc tgtggtgtct
    23821 ttctcatctt tcagaatcag gttgatgcga gtaatcaggc tatcttcgat aaactcaatc
    23881 aggctatcga acatgcgggt cttactgggg aagtggcgat acagtgccgc ttcggaaacg
    23941 ccgacagagg cggccagttt tgccgtcgtg atacgttggc ttccatcgct ggattccagc
    24001 atcagcgcca gagactgaag tatttcctcg cgacggttcc ttttcgcagt ttgtttttct
    24061 gccatgttac aaaatacccc tgaaaataag cacttgccag gcggcaccca cgctatgacc
    24121 gcaaacgaaa tgtttgcggc tatgttatga cgttattcgg atgcgtatgt gttactgacg
    24181 accagagtga ccaaagccgc cttcaccgcg gtcggtggcg tcgaaatctt ccaccagatt
    24241 aaattcagcc tgtactaccg gaacaaaaat catctgggcg atgcgttcgc caggttgaat
    24301 ggtgaagctg tcctgaccac ggttccacac ggaaatcatc aactggccct gatagtcaga
    24361 atcgatcaat cctaccaggt taccaagcac gataccgtgc ttatgtccca atccggagcg
    24421 cggcagcatc attgccgcca gtgaaggatc ggcaatatga atcgccagcc cggtcggaac
    24481 cagcgtagtg tcacccggag ccagttctac ggcgtcgtcg agacaggcac gcaggtcaag
    24541 tccggcagag ccagaggtgg cataagtcgg gagcggaaat tccttcccaa cgcgcgggtc
    24601 cagaatctta acgtcgattt ttttcatcat aacgggtcac gatctcgtcg agtaataatt
    24661 ggccaaggag ctctttgcgc tcaagcggta agactttatc tccgtcctgc cagaaaaggt
    24721 gtaatgcgtt gttgtcgctg ttaaatcctt gagttggctg ggaaacatcg ttcgcgcaga
    24781 tcagatcaag gtttttacgg atacgttttt gccgggcgta ttcttccaca ttatttgttt
    24841 cggcggcaaa tccaacgacg tagggtcgat ggtcttttag tgcggcaacg cctgcgacga
    24901 tatcggggtt tttaaccatt tttattgtta attcatcacc ctgcgtggcc tgctttttga
    24961 ttttctctgg ggccacggta gctgcgcgat aatccgccac ggcggcgcag ccgataaaaa
    25021 tattttgctg ctgtacagaa gcattcacgg cggcttccat ttccagcgca gtcatcacat
    25081 caacacgttt aacaaacggt ggcgtcggta gtgaaaccgg acctgatacc agcgtgacgt
    25141 tcgcgccacg acgggcagcg gcggcggcga tagcaaaacc catcttgccg gagctgtgat
    25201 tagagatata acgcaccgga tcgagcggtt cacgcgtcgg accggcggta atcataatgt
    25261 tcagatgttt caggtcgttg acgggcgaaa aatgcgccac cgccatatcc acaatggtta
    25321 acggatcgag cattcgccca ggaccgatat caccacaagc ctgactgcca ctgtctggcc
    25381 cccagatgag caaaccacgg gaagcaagca cctctaaatt atgctgcgtg gcagcggcac
    25441 ggtacatctg ctggttcatg gcggggagca cggctacagg cgcaggtgta gccagacaaa
    25501 tcgtcgatac caggtcattc gccattccgg cagcaacacg ggcaatcaaa tctgccgtgg
    25561 caggggcgag aatcactaaa tcagcccatt tacccagctc aatatggccc atagcggctt
    25621 cggctgccgg gtccagcaaa ctgtcggaaa cgggataacc agaaaccgcc tgcaagctaa
    25681 gtggggtgat aaaggctttt gccgcttcgg tcatggctac gcggacgtcg gccccgcgat
    25741 cgcgcaaacg acgcaccagt tcaggggttt tataggcagc aataccgccg ctaacgccga
    25801 gaacgatttt tttaccggcc aggctcatga tgatttttcc tgttgggtga caccagaagt
    25861 tggcgatttt atcacaatac ttttgttgtc gtgccttcac ctgagaatca ctttgcgagg
    25921 cgctttccag gattgaaaac tggccgtcga tttaacggaa cggctatgac aggatgcgag
    25981 caccacaaag gaggtgaagg tgaaaaacaa tgcacagctg ttgatgccgc gcgaaaaaat
    26041 gctgaagttt ggtattagcg ccttaacgga tgtcgagctg ctggcgttat ttctgcgtac
    26101 cggaacgcgc ggtaaagatg tattaaccct ggcaaaagag atgctggaga atttcggctc
    26161 tctttatggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
    26221 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
    26281 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
    26341 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
    26401 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
    26461 aattatccgc gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
    26521 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
    26581 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
    26641 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
    26701 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
    26761 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
    26821 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
    26881 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
    26941 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
    27001 ggttgagagc gagaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
    27061 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
    27121 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
    27181 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
    27241 ctggaactga aaaaattcga tccagttgtt cgtcagcacg tgatctacaa agaagcgaaa
    27301 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
    27361 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
    27421 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
    27481 tgcagtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
    27541 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ttgctgctgg agctgcctga
    27601 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
    27661 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggtaaag tgctgcgcta
    27721 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcataatgt
    27781 gctggcccat cttggaccgg agccgctcag cgacgatttc aatggtgagt atctgcatca
    27841 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
    27901 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
    27961 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
    28021 gttgctgcgt tcgattgagc agggcggtac aacgctgaaa gattttctgc aaagtgatgg
    28081 taaaccgggc tatttcgcac aggaattgca ggtttacggg cgaaaaggcg agccgtgtcg
    28141 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
    28201 gcagtgccag aagtaattca tgcgcgccgg atggcatacc atccggcata aacgctacgc
    28261 taacttcgcc atcagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
    28321 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
    28381 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
    28441 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
    28501 ggcgaagttc gccatcaaat cactaaaccc gaccacttcc acgttcccca gatgcgcggt
    28561 tgcctgctgt gccagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
    28621 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
    28681 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
    28741 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
    28801 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
    28861 cggtgagtaa agaggacacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
    28921 cgcttgcctg ctccagccgc gcgcaaatgt ctttaaagtt aaaagtatgc ggccccatca
    28981 ataccggaat agcgtgtgcg gcagcttcca gcggattatg cccaccacgt tcaaccagtg
    29041 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
    29101 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
    29161 ttagtccagc ctggcggaca agattaatcg catccgggaa gcgttctgga tgacggggta
    29221 ccaggatgag caataaattc gggaattgct gtaacaatgc ctgatgtgcg gcgatcacca
    29281 cactctcttc gccttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
    29341 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
    29401 ggctaccggt aacggtcacc tgattatttt ttgcgcccag cgccacaaaa cgtgcaccat
    29461 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag caagcgacgg acgaatttac
    29521 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
    29581 gaattttacg tttgtgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
    29641 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
    29701 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
    29761 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
    29821 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
    29881 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
    29941 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
    30001 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
    30061 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
    30121 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
    30181 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
    30241 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa atcattcggg tcggattgcc
    30301 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
    30361 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
    30421 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
    30481 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
    30541 aaatcgatgt gctgctttat caggacacca tcccgatcct gtctgaaaat ccagagatta
    30601 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
    30661 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat ctcaccgatc
    30721 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
    30781 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttggtg ccgttgcagg
    30841 gtggaaatgt ggtggaaagt aacttatccg tgctgacccc attgggagtt gattcgttgg
    30901 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
    30961 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
    31021 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
    31081 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
    31141 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
    31201 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
    31261 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagatcat attttctggc
    31321 gtccctggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
    31381 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
    31441 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
    31501 ttgtttatat aaatattttc cctttggcgg tttgcagcgt gattttatgc gtattgctca
    31561 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
    31621 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
    31681 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
    31741 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
    31801 gaaagtagcg caggaaaaag gctttttcta tcgcctgacg tcacgttatc gccattatgc
    31861 cgcctttgag cgggcaacct tcgaacaggg taagccgaca cagctgctga tgctgacaga
    31921 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
    31981 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagccaata gccgtgaaat
    32041 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
    32101 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
    32161 gcgccacaac acattgctat atgttgttgg gcaggataaa ccgcgaaaat ttgaggcgct
    32221 ggcagaaaaa cgcggcgtgt gcagtaatgt tcactttttc tcggggcgca atgatgtctc
    32281 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cggcgggaat
    32341 tgtgctgctg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
    32401 tgcgccttat attgtcgagg ctaattgcgg cgaggctatt gctgagccat tccgccagga
    32461 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
    32521 ggaaaatgcg cgatattatg ctgatacaca agatttatac agtctgccag agaaagcggc
    32581 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
    32641 gcggcaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggtattt cgtgaactgg
    32701 aaactcgccg tactctgcgc tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
    32761 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
    32821 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgc
    32881 atggggtggc atttggcgaa aaaggcatga atccgctgac cagaacttca tttattatta
    32941 ccgaagatct gacaccaacc ataagtctgg aagattactg tgctgactgg gcgactaacc
    33001 ctccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
    33061 tgcatgctgc gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
    33121 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
    33181 ttcgcacgcg cgttccacgt cgttggcggg ataaagatct tattgggctt tatttttctt
    33241 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgccg
    33301 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
    33361 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta acccgtaact tatttttgcc
    33421 aaaattttgg atacagaata aatatgtctc aactcaatga t
//
LOCUS       DAZFKM010000040        33414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFKM010000040 DAZFKM010000000
VERSION     DAZFKM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..690)
                     /locus_tag="ACHKAY_002032"
     CDS             complement(<1..690)
                     /locus_tag="ACHKAY_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5700712.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(701..1702)
                     /gene="ltaE"
                     /locus_tag="ACHKAY_002033"
     CDS             complement(701..1702)
                     /gene="ltaE"
                     /locus_tag="ACHKAY_002033"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO5700713.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1739..3457)
                     /gene="poxB"
                     /locus_tag="ACHKAY_002034"
     CDS             complement(1739..3457)
                     /gene="poxB"
                     /locus_tag="ACHKAY_002034"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO5700714.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3590..4558)
                     /gene="hcr"
                     /locus_tag="ACHKAY_002035"
     CDS             complement(3590..4558)
                     /gene="hcr"
                     /locus_tag="ACHKAY_002035"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO5700715.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4570..6222)
                     /gene="hcp"
                     /locus_tag="ACHKAY_002036"
                     /gene_synonym="priS"
     CDS             complement(4570..6222)
                     /gene="hcp"
                     /locus_tag="ACHKAY_002036"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO5700716.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6366..7265)
                     /gene="lysO"
                     /locus_tag="ACHKAY_002037"
     CDS             complement(6366..7265)
                     /gene="lysO"
                     /locus_tag="ACHKAY_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO5700717.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7760..8455)
                     /gene="aqpZ"
                     /locus_tag="ACHKAY_002038"
     CDS             complement(7760..8455)
                     /gene="aqpZ"
                     /locus_tag="ACHKAY_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO5700718.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8881..10539
                     /gene="ybjD"
                     /locus_tag="ACHKAY_002039"
     CDS             8881..10539
                     /gene="ybjD"
                     /locus_tag="ACHKAY_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO5700719.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10536..11492)
                     /gene="ybjX"
                     /locus_tag="ACHKAY_002040"
     CDS             complement(10536..11492)
                     /gene="ybjX"
                     /locus_tag="ACHKAY_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO5700720.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11643..12758
                     /gene="macA"
                     /locus_tag="ACHKAY_002041"
     CDS             11643..12758
                     /gene="macA"
                     /locus_tag="ACHKAY_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO5700721.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12755..14701
                     /gene="macB"
                     /locus_tag="ACHKAY_002042"
     CDS             12755..14701
                     /gene="macB"
                     /locus_tag="ACHKAY_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO5700722.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14774..14998)
                     /gene="cspD"
                     /locus_tag="ACHKAY_002043"
     CDS             complement(14774..14998)
                     /gene="cspD"
                     /locus_tag="ACHKAY_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO5700723.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15148..15222
                     /gene="yljB"
                     /locus_tag="ACHKAY_002044"
     CDS             15148..15222
                     /gene="yljB"
                     /locus_tag="ACHKAY_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO5700724.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15321..15641
                     /gene="clpS"
                     /locus_tag="ACHKAY_002045"
     CDS             15321..15641
                     /gene="clpS"
                     /locus_tag="ACHKAY_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO5700725.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15672..17948
                     /gene="clpA"
                     /locus_tag="ACHKAY_002046"
     CDS             15672..17948
                     /gene="clpA"
                     /locus_tag="ACHKAY_002046"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO5700726.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18291..18378)
                     /locus_tag="ACHKAY_002047"
     tRNA            complement(18291..18378)
                     /locus_tag="ACHKAY_002047"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18342..18344),aa:Ser,seq:gga)
     gene            complement(18632..18850)
                     /gene="infA"
                     /locus_tag="ACHKAY_002048"
     CDS             complement(18632..18850)
                     /gene="infA"
                     /locus_tag="ACHKAY_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO5700727.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(19135..19839)
                     /gene="aat"
                     /locus_tag="ACHKAY_002049"
     CDS             complement(19135..19839)
                     /gene="aat"
                     /locus_tag="ACHKAY_002049"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO5700728.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(19881..21602)
                     /gene="cydC"
                     /locus_tag="ACHKAY_002050"
     CDS             complement(19881..21602)
                     /gene="cydC"
                     /locus_tag="ACHKAY_002050"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO5700729.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(21603..23369)
                     /gene="cydD"
                     /locus_tag="ACHKAY_002051"
     CDS             complement(21603..23369)
                     /gene="cydD"
                     /locus_tag="ACHKAY_002051"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO5700730.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(23492..24457)
                     /gene="trxB"
                     /locus_tag="ACHKAY_002052"
     CDS             complement(23492..24457)
                     /gene="trxB"
                     /locus_tag="ACHKAY_002052"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO5700731.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            25001..25495
                     /gene="lrp"
                     /locus_tag="ACHKAY_002053"
     CDS             25001..25495
                     /gene="lrp"
                     /locus_tag="ACHKAY_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO5700732.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            25630..29736
                     /gene="ftsK"
                     /locus_tag="ACHKAY_002054"
     CDS             25630..29736
                     /gene="ftsK"
                     /locus_tag="ACHKAY_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO5700733.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            29891..30502
                     /gene="lolA"
                     /locus_tag="ACHKAY_002055"
     CDS             29891..30502
                     /gene="lolA"
                     /locus_tag="ACHKAY_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO5700734.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            30513..31856
                     /gene="rarA"
                     /locus_tag="ACHKAY_002056"
                     /gene_synonym="mgsA"
     CDS             30513..31856
                     /gene="rarA"
                     /locus_tag="ACHKAY_002056"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO5700735.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            31947..33239
                     /gene="serS"
                     /locus_tag="ACHKAY_002057"
     CDS             31947..33239
                     /gene="serS"
                     /locus_tag="ACHKAY_002057"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO5700736.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
BASE COUNT         8323 a         8482 c         8929 g         7680 t
ORIGIN      
        1 gcggcgctta ccgctcaccg ccataaaatg ttcaaactgt tgctgataac tgagcacctc
       61 tggtccggcg gcttcgaaga tgcggtgttc gctggccgga tgatctaaca acgccaccag
      121 atagtgcagc aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc agcgtggcgg
      181 cgttaacact ggcaggttgt agaccatatc gcgcatgact tcgaacgccg ctgaacctgc
      241 gccaacgata attccggccc gaagttcggt cacaggtaca ttcgcttcac gaagaatgtc
      301 cgccgtagcc tgacgagcac gcagatgatc cgactgctca tgtggcgggg cctgcaacga
      361 actgagaaag attaattgct taactggtac ttcacgtagc gcatcgcgga cgttgagagc
      421 cacctggcgc tcctgagcga taaaatcgcc gccttcgccc atgctgtgca ccagaaaata
      481 gacggtatcg atatcctgca acagggccgg aaggttatcc ggccagctga gatcgacttt
      541 atggcaactg acgtttgcca gttgcagctt tgcaagcctg tcgacatgac gtgccgccgc
      601 caggatctga tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa tgtagccact
      661 ggcaccgaga actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc aggaatgcgc
      721 gccagtgggc ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc gtcaccaggc
      781 ggacaatcgg cgaggcgtta atcagcacgt ttctcgcttt catgtattcg cctaacgcgg
      841 cagcattttc ttccccgacg cgaacaaaca gcatattagt gtcctgacgc atcacatccg
      901 cgcctgcttc acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct tcctgcaacc
      961 gtgcgacgtt atttttcagg gcatacatcc cggcggcagc cagaatcccg gactgacgca
     1021 tcccgccacc ggccattttc cgccagcgga tggcacgttt aatgtaatca cgattaccga
     1081 cgagtaatga accgactggc gtcccaagac ctttcgacag gcaaatggtg aacgaatcac
     1141 aatattgcgt gatctctttc agttcgcagc cgtaagccac cacggcatta aagatgcgcg
     1201 caccgtcaac atgcagcgcc agattgcgct cgcgggtaaa ttcccatgct tctttcaggt
     1261 attcacgcgg cagcactttg ccgttgtggg tgttttccag actgagtaat ttggtgcggg
     1321 cgaaatggat atcgtcgggt ttgattttca tcgccacttt atccagcggt agcgtgccgt
     1381 cggcagccgc gtcgatgggt tgcggctgaa tactgcccag caccgctgcg ccaccggctt
     1441 caaacagata gttatgcgcg gcctgaccga caatatactc ttcgccacgt tcgcagtgac
     1501 tgagcagagc gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg gcttctttac
     1561 cggaaagctc tgctgcatag tcctgcagag cattaacggt agggtcgtct ccgtaaacgt
     1621 cgtccccaac cggggcggcc atcatcgctt cgagcatggc gcggctcggt cgggtaacgg
     1681 tatcactgcg taaatcaatc atggcatgtc cttattatga cgggaaatgc cacccttttt
     1741 accttagcca gttcgttttc gccagttcga tcacttcatc gccgcgtccg ctgataattg
     1801 cgcgcaacat atacaggcta aaacctttgg cctgttcgag tttgatctgc ggtggaatgg
     1861 ctaactcttc tttggcgacc accacatcca ccaacaccgg accgtcgata gagaaggcgc
     1921 gttgcagggc ttcatcaact tcagacgctt tttctacacg gatacccgta atgccgcacg
     1981 cttcggcaat gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc aaatagccac
     2041 cagctttcat ctccatcgcc acaaagccca gcacgctgtt gttaaagacg acaattttca
     2101 ctggcagttt catctgcact actgagagga aatcgcccat caacatgcta aaaccgccat
     2161 cgccgcacat ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc agcgcctgcg
     2221 gcatggcgtt agccatcgaa ccgtggttaa acgaacctaa caggcgacgc ttgccgttca
     2281 tttttagata acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg aaaatagcgt
     2341 catcggcggc aaaatgacta atttgctgcg ccagatattg cgggtgaatg gctttctcgc
     2401 tcggtttagc taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc agcgctttat
     2461 ccagaaactt gcgatcggct ttttcttcca ccaatggaag caatgcacgc agagtcgact
     2521 tgatatcgcc gaccagtgcc atatccacct tgctgtgagc gccgatgctg gctgggttga
     2581 tatcaatctg aatgattttg gcatcggtcg ggtagaaggc gcggtaggga aattgcgtgc
     2641 cgagtagcac taacgtgtcg gcgttcatca tggtatggaa acctgacgag aagccgatta
     2701 acccggtcat tccaacatca tacggattat cgtattcgac atgttcttta ccgcgcaggg
     2761 catgaacaat aggcgcttta attttcccgg caaactcaac taactcttta tgcgcccccg
     2821 cgcagccgct gccacacatc agggcgatat tgctggaata acgcagcagt tgcgccagtt
     2881 tgcgtaactc ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac cagtgcgtgg
     2941 ttgccccttc tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca accgaaacgc
     3001 cacggttaag caccgctttg cgcatggcaa tcgccagtac ttgtgggatc tgctccgggc
     3061 tggaaaccag ctcgcaatag tgactacatt cgcggaatag ctcttgtggg tgggtttcct
     3121 ggaaatagcc gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc agtaccggaa
     3181 cgtgattgcg gtggcaatcg aacaggccgt tgattaagtg caggttgccg gggccgcacg
     3241 atccggcgca gaccgccagt tctccgctaa gttgtgcttc agcgccagcg gcaaaggccg
     3301 ccacttcttc gtggcgggtg gacatccact cgatggtgcc catgcgatta agactgtcac
     3361 taagaccgtt cagagagtcg cctgtgactc cccagatgcg tttcacccct gccgattcga
     3421 gtgttttggc gatataagct gcaaccgttt gtttcatggt tctccatctc ctgaatgtga
     3481 taacggtaac aagtttagtt catctgacgg agggggaagg gatgggagag aaaggaggca
     3541 ctaacggtta aatagcccga tgaaaggaat atcatcgggc ataaggcgat tatgcgagaa
     3601 ccaaatcccc ctgcggatgg caggagcagg ccagtacgta accttcagcg atttcggcgt
     3661 cggtcagcgt cattgtactg ctcaccgtat attcaccgga aaccactttt gtcttacagc
     3721 agccgcaaac acccgcacgg caggcagcga caaccggaac gttattgctt tccagcgcct
     3781 ccagtagcgt ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt ttggtgaatt
     3841 tcagaccgct ggtcgccgct tctgctactg gggtgaagaa tttctcttta aagaaacgcg
     3901 tcacgccgag cgctttcact tcctgctcta cccaatccat atacggagcc gggccgcagg
     3961 tcatcacggt acgtgaagct aagtcaggta cacctgccag cagttcgcga gtgagacgac
     4021 cagcgataaa gccttcggta acgttatttt ctgccaccag cgttaccgga tagttacgcc
     4081 actcatcggc gaaaataaca tcctgtggcg tacgcacgtt gtagatcacc cgcacatcgg
     4141 cctgtggacg gttcttcgca agccagcgac gcatcgacat aatcggcgtg acgccgcagc
     4201 ctgccgccag caacaggaat ttatcttctg ctttatcgtc gcaggtaaat tcccccatcg
     4261 cgtccgaaag ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac tgggagccga
     4321 caccgtcatc aatccgccgc acggtcaggg tgatatattc actcacgcct ggcgtggagg
     4381 aaatggtgta agcacgcagc gtttccgctg agtttcgcac gctgaccagt gcatattgcc
     4441 cggcgcgata tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca tccggcgttt
     4501 cttgcgtaat gtgatgaacc tgcatccgcc acgggcattg attcgttggc atcgtcatcg
     4561 acaaactcct tacgcgctca acagttgctt catgtcttct tcaacagtgg tgatagaacg
     4621 caggccaaat ttctcgttca gcaccgccag caggtctggt gtcaggaaac caggtgcagt
     4681 cgggccggtg acgatatttt tcacgcccag agaaagcagc gtcagcagaa tgacgatcgc
     4741 tttctgttca aaccaggaga gcaccagcga cagcggcaga tcgttgacac cgcagcccag
     4801 tttctctgcc agagtgacag ccagaataat cgctgagtaa gcatcgttac attgacctgc
     4861 atctaccaga cgcggcagac cttcgatatc gccaaactca agtttgttaa agcgatattt
     4921 accacaggcg agggtcagga tcaggcagtc atccggcacg ctggtggcga aatcggtgaa
     4981 gtagtggcgc tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat gacgcagttt
     5041 ttcacggctc accagatcaa tcagcgtatc agcagcgcca agcagcgtct ggcgaccaaa
     5101 acccacggtg ataaggtgcg gaatttcgct gtacgggaag cctgccatct gttgcgcctg
     5161 ggtgataacc gcagagaaat catcaccgtc cagatgacgc acgccaggcc agccaacaat
     5221 gctgcgggtc cagatacgat cgtcataagc gcctacggtt gggtcgatga tgcagttcga
     5281 ggtcatcacg atggggccag ggaaacgagc gaactccact tgctgattct gccagccact
     5341 gccgtagtta ccgaccagat gcttgaattt acgcagctcc gggtagccat gcgcaggcag
     5401 catttcgccg tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca gcaggttgta
     5461 gagatctttg agatcgtgac cggaaatcag aatgcattta cccgccgtcg ctttgacgtt
     5521 gacctgggtt ggcgtcgggt gaccgtattt accggtttcg cctgcatcca gaatgctcat
     5581 cactttgaag ttcatctggc cgatttccat tgaacactca agaagcgcgt tcatatcggc
     5641 aggccaggtc cccagccacg ccatgatttt atgatactgg gcataaatat cgttgtcgta
     5701 ttgaccgaga acatgcgcgt gttccatata ggccgccgca cctttcaggc catacaggca
     5761 cagcagacgc aggccgagaa tgttttcgcc aatcgccgct ttatctttgt taggggtaaa
     5821 ttctgcagcc tgacgttgca gctcgccgag atcgtcgctc accagttgca ggtcagccat
     5881 cgggttatcg acgcgcgcgt tggcatctac agccaggcat tgcgctttca gcgcctcgcg
     5941 cagggcaatc gcttcacgag cgtagccgac aatacgcgga gaatcgaagt taacgttggt
     6001 cagagttgag aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga tgatgccgta
     6061 ttcacgcgct tttaccgccc aggcagaaag cccttgcagc gccgcgatga gtaaatcctg
     6121 aaggtcagaa gtttccgccg ttttaccaca catcccctgc gcgtatgagc agccgtttcc
     6181 tgccggagta cggatagttt gttcacattg cacacaaaac atgatcacac cttttaaagt
     6241 tatatttaat atacatgttt aaggttaaga cgcttaacgc ggggataaaa gggatttttc
     6301 atgcaacttt aagggagatt gatttagcgc aattttggcg gcaggaatct accgccagag
     6361 aggtattacg cagagaaaaa ggcgatgagg atcggcacta acaggctaag aataaaaccg
     6421 tgaacaattg ccgccgggac catatccagc ccgccagtac gttgaagaac gggcagggtg
     6481 aaatccattg atgtggcacc gcataagccc agtgcagtag agcggctgcg gcgaatcagc
     6541 ccagggatca acataatagc aatcagttca cgggccagat cattaaaaaa cgccgcgctc
     6601 ccgattaccg gaccaaaaga ttcggtcaat aaaataccgg aaagagaata ccagccgaaa
     6661 ccggaggcca ttgccagcga ggtattgatg gggagatcaa gaataaaggc gttaattaaa
     6721 ccaccaatta atgaactgac aaccaccacc acggcgacaa tcattccccg gcgattaagg
     6781 acaatctgct ttaaggtcat gccattattg cgcaactgaa taccaacgag gaaaagtagc
     6841 aaaattaacg tgtattcact ggcttcggtc gcgtgttgta agaaagccag tccactcaga
     6901 ccaatggcaa aaccaatcac tactacgccg cacagtttta gcgactccag cgccatcgca
     6961 atacgcgacg ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc tcgctccagc
     7021 cacatcaggg cggcaatatt acacagtaaa ataacggtaa tactgacggc agaataatgc
     7081 agaatcgcca acaggttact ggcgaggtta tcgaggaacg ccagacttat acccataaaa
     7141 aagagaataa ggtaaaccat ccagcttaat agctgattaa taacttttaa cgcagcttgt
     7201 tggcgaagcg gaatgaggta acccacaatc aggggaacca gaatgattaa cagcccagaa
     7261 aacatgaaaa cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca cactactgaa
     7321 agcggaagga cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg agcagtagag
     7381 tattcgtttt catttaaaga tattcttgcg ctttaattac aaactgcacc gatgttggtg
     7441 gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga
     7501 gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagtcg
     7561 agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggtgcatgg ggcggcggcg
     7621 attggccgcc ccctgcgcgc tccttgcgcc agtggcaata tgtggcttag ctcatgaaag
     7681 gagcgcaaca agatgatgaa tcaacatata acaacatctt aaaaaaaggc ctgacattac
     7741 gccaggcctt ctgcgttaat taatcacgct tttccagcag ggtccggtaa atcagaccac
     7801 cgataatgcc gccgacaatt ggcaccaccc agaagaacca cagttgttct aatgcccagc
     7861 cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg ttagtcaccg
     7921 gaatactaat taagtgaatc agggttaagg ccagaccaat agcgatcggc gcaaaacctg
     7981 ccggcgcgaa tttgtcggtt gcgccgtgga tcaccaacag gaaacctgca ctcaatacca
     8041 gttcaactac cagcgcggaa agcatggaat aaccgcctgg tgaatgctcg ccataaccgt
     8101 tagaagcaaa accgctggct gccgcgtcaa aacccgtttt accactggca attaaataca
     8161 gcagcgccgc tgcaacaata ccgccgacaa cctgggcaat tacgtagcca acgacttctt
     8221 ttgccggaaa acgtccgcca gcccataaac caatagtgac cgccgggtta aaatgaccac
     8281 cagaaatatg accaacagca aaggccatcg tcagaacggt cagaccgaac gccaacgcca
     8341 cgccggcaaa accaatgcct aattccggga agcctgcggc cagtacagca ctaccacagc
     8401 caccaaaaac aagccagaaa gtaccaaaac attcagctgc taattttctg aacatatcca
     8461 cctcaattaa aaattgaccc tgtgaaaaat atggtcgttt tatagggtcg tcgtaaaaaa
     8521 gtgacgacgg aaataatgcg cggctatttt aaaaacgaag gcgagtcatt caccagataa
     8581 ataaatccag taaatttgat ttagggcaac agcgggttgc cccatatagt catttgtctg
     8641 attgacagtg tagtgcaagc aaaagattta atcctttagg cgtaataaaa aataatttat
     8701 catgctaatt atttgatttt gttgtttttg cagacttatc agcaagaggg agtataacgc
     8761 gattattcgc tcatttttca gacatttgcc atgcttaaat gtgatgtcat cacgtattag
     8821 caaggccttt cccgttatac tgccagcgta aaggataagt cacatatttc tggaggggat
     8881 atgattcttg agcgcgttga aattgtgggt tttcgcggta tcaaccgttt gtcgttgatg
     8941 ctcgaacaaa acaacgtcct gattggggag aacgcgtggg gtaaatccag cttgctggac
     9001 gccttaaccc tgctgctatc gccagaatca gatctctacc attttgagcg cgacgatttc
     9061 tggttcccgc cgggagatat caacgggcga gaacatcatc tgcatattat tttgaccttc
     9121 cgcgaatcgc tgccaggccg acatcgggtt cgccgttatc ggccgctgga agcgtgctgg
     9181 acgccatgca ccgatggcta tcaccgtatt ttttatcgtc tggaagggga gagtgcggaa
     9241 gacggcagcg tgatgacact gcgcagtttt ctcgataaag acggacatcc gattgatgtc
     9301 gaggatatta acgatcaggc acgccatctg gtgcgtttaa tgccggtgct gcgcttgcgt
     9361 gatgcccgtt ttatgcgccg tattcgtaac ggcacggtgc caaatgttcc caatgtggaa
     9421 gtcaccgcgc gccagctcga tttcctcgcc cgtgagttgt cctcacatcc gcaaaatctc
     9481 tctgatggac agattcgtca gggactttcc gcaatggtgc agttgcttga gcattatttc
     9541 tctgagcagg gggctggaca ggcgcgatat cgtttaatgc ggcggcgggc cagcaatgag
     9601 caacgaagct ggcgctatct ggacatcatc aaccggatga ttgaccgacc tggagggcgc
     9661 tcgtatcggg ttattttgct cggcctgttt gctactttat tgcaggcaaa aggcacattg
     9721 agactggata aagacgcgcg tccgttattg ctaattgaag atccagaaac ccgtttacac
     9781 cccattatgc tttcagttgc ctggcatctg ttgaatcttc tgccattgca gcgcattgcc
     9841 accaccaact cgggtgagtt gctttcgtta acgccggtag agcatgtttg ccgactggta
     9901 cgtgagtcct cgcgcgttgc cgcctggcgt ctggggccga gtggcttgag taccgaagat
     9961 agccgacgca tatcctttca cattcgtttt aaccgtccgt catcgctgtt tgctcgctgc
    10021 tggttgctgg tggaagggga aacggaaacc tgggttatca atgaactggc gcgccagtgt
    10081 ggacaccatt tcgatgccga agggatcaag gtcattgagt ttgcccagtc cgggctaaag
    10141 ccactggtta aatttgcccg ccgaatgggg attgaatggc atgtactggt cgatggcgat
    10201 gaagcaggga agaaatatgc cgctacggta cgcagcctgt tgaataacga tcgggaagcc
    10261 gaacgagaac atttaacggc gttaccggcg ctggatatgg aacattttat gtatcgccag
    10321 ggattttccg atgtgttcca ccgcgtggcg caaatcccgg aaaatgtacc gatgaatctg
    10381 cgcaaaatta tctcgaaagc gatccatagc tcttccaaac ccgatcttgc cattgaagtg
    10441 gcaatggagg ccggacgtcg tggtgtggac tccgtaccga cgctgctgaa aaaaatgttc
    10501 tcacgcgtgc tgtggctggc gcgcggtcgc gcggattaac cgcgaaacat cgtggccatt
    10561 tgtggctgaa tagcgtcgag catctcatag cgccgacggt attcagcccg ttttttactg
    10621 gcgatttcgg caatctcttt tcgtgctatc tgtgctggaa ggcggtaatg gcgttcagca
    10681 tcacatacgc cgccaaccga ttcccagaaa gcgttgtaat cggcatggat cttgccttct
    10741 ttatcacgat aacgcaggct gcggtaaata tgcgtttcat tgctgacggc aataatctgc
    10801 tctacctgca aacgttgggc aaacagacag gccgcttcca tcacgaggcg tttgggaaat
    10861 agcccgtggc aggctttcgt cgcattctgg atttcctgat gtgggatttc ccattttgcg
    10921 ccttgcagtc cgccaataaa catcgttctt ttcccctgat attcacacag ggtaaaagtg
    10981 agctctgcca gaggaatacc ttcgctgttg cggaacagga ttgtgctgtc accttcttta
    11041 tccattgaga tcatcatggt cagctcaagc gtgaactgct cgccgttttt gccttccagt
    11101 ttagccagtt gcagcccggg ggtattcaaa tataagctga attcttccgc cgacatacat
    11161 ccgcggagta acgcataatg gtaacgtaac gcctccagca attgcttacg gctaagattc
    11221 gccgcaaggt aagggcgatg cagacgcaca ggcagtcgcg gttggcgcgt taacaacaca
    11281 ttgagattag gccagtggga aagttcgttc atccactcaa cgcttaaacg cggcataatc
    11341 aacgagcgca gcaaaaattt ctggcgaaaa ctacggcgat gccagaattt acccggccga
    11401 cactgtccac gtgccagact aagaaaaagt gacaggctgc tgagagattc agatggcgta
    11461 aaggtccgtt cagttagctg cgacatattc atgaaatcaa tggttataca tgacgtcgat
    11521 ttcaccattg cgtatcttaa ccaaacatca atagtgtgat tactaacgta aattttaggg
    11581 ttttgttgat atttcgttga agttaatgac ccggattggc atatggagta ttcagaatat
    11641 ttatgaaaaa gcggaaaacc gtgaagaagc gttacgttat tgcgctggtg atagtcatcg
    11701 ccggactgat tacgttatgg agaattctta acgcacccgt gccgacttat cagacactga
    11761 ttgtgcgccc cggtgattta cagcaaagcg tgctggcgac cggaaagctg gacgcgctgc
    11821 gtaaggttga cgtgggcgcg caggtcagcg gtcagttgaa aactctgtcg gtggcgattg
    11881 gcgataaagt aaaaaaagac cagcttttag gggttattga tcctgaacag gctgaaaacc
    11941 agattaagga ggtcgaagca acgctgatgg agctacgtgc gcagcggcag caggcggaag
    12001 cggagctgaa actggcgcgg gtgacgtatt cccgtcagca acgtctggca caaacgcagg
    12061 cggtttcaca gcaggatctc gacaccgccg cgacggagat ggctgtgaaa caggcgcaaa
    12121 ttggcaccat tgacgcgcaa atcaagcgca atcaggcctc tctcgatacg gctaaaacca
    12181 atctcgatta cactcgcatc gttgccccga tggccgggga agtcacgcaa atcaccactc
    12241 tgcaaggcca gacggtgatt gccgcacaac aagcaccgaa cattctgacg ctggcagata
    12301 tgagcaccat gctggtaaaa gcgcaggttt ctgaagcgga tgtaatccac ctgaagccgg
    12361 ggcaaaaagc ctggtttacg gtacttggcg atccactgac gcgctacgag gggcaaatca
    12421 aggatgtact accgacgccg gaaaaggtta acgacgctat tttctattac gcccgttttg
    12481 aagtccccaa ccccaatggt ttgctgcggc tggatatgac tgcgcaagtg catattcagc
    12541 tcaccgatgt gaaaaatgtg ctgacgatcc ctctgtcggc gttaggcgat ccggttggcg
    12601 ataatcgtta taaagtcaaa ttgttgcgta atggtgaaac acgcgagcgt gaagtgacga
    12661 ttggcgcacg taacgatacc gatgttgaga ttgtcaaagg gcttgaagcg ggcgatgaag
    12721 tggtgattgg tgaggccaaa ccaggagctg cacaatgacg cctttgctcg aattaaagga
    12781 tattcgtcgc agctatcctg ccggtgatga gcaggttgag gtgctgaagg gcatcaccct
    12841 cgatatttat gccggtgaga tggtagcgat tgttggcgct tcaggttccg gtaaatcgac
    12901 cctgatgaat attctcggct gtctggataa ggccaccagc ggcacctatc gcgtcgccgg
    12961 tcaggatgtt gccacactgg acgccgatgc gctggcgcaa ctgcgccgcg agcatttcgg
    13021 ctttattttc cagcgttacc atttactttc gcatttaacc gccgagcaga acgttgaagt
    13081 acccgccgtc tatgctggtc ttgagcggaa acagcgactg cttcgtgccc aggagttgct
    13141 gcaacggctg gggctggaag accgtacaga gtattatccg gcacagcttt cgggtggtca
    13201 gcaacagcgc gtcagcatcg cgcgggcatt gatgaacggt ggtcaggtaa ttcttgccga
    13261 tgaaccaacc ggcgcactgg acagccattc tggcgaagag gtgatggcga tcctgcatca
    13321 gctgcgcgat cgtgggcata cggtgattat cgtcacccac gatccgcagg tcgctgctca
    13381 ggccgagcgg gtgatcgaaa ttcgcgacgg cgaaattgtg cgcaatcctc ccgccattga
    13441 aaaagtgaat gttgctggcg ggacggagcc tgttgtcaac acggtgtctg gctggcggca
    13501 gtttgtcagc ggttttaacg aggcgctgac gatggcatgg cgggcgctgg cagcgaataa
    13561 aatgcgtact ttactgacca tgctggggat tattatcggt attgcgtcgg tggtttccat
    13621 tgtcgtggtg ggtgacgccg ccaaacaaat ggtgctggcg gatattcgtt ctattggtac
    13681 gaatactatt gatgtctatc ccgggaaaga ttttggcgat gacgatccgc aatatcagca
    13741 ggcgctgaag tacgacgact taatcgccat ccaaaaacaa ccgtgggtcg cctcagccac
    13801 acctgccgtc tcgcaaaacc tgcgcctgcg ttataacaat gttgatgttg ctgccagtgc
    13861 caatggcgtg agcggcgatt attttaatgt ctatggcatg accttcagtg aaggaaacac
    13921 ctttaatcag gagcagctga acggtcgtgc gcaggtcgtg gttctcgaca gtaatactcg
    13981 ccgccagctt ttcccccata aagcagatgt ggttggcgag gtgattctgg tcggcaatat
    14041 gcccgccaga gtcattggtg tggcggaaga aaaacagtcg atgtttggta gcagtaaagt
    14101 gctgcgtgtc tggctacctt acagcacgat gtccgggcga gttatgggcc agtcgtggct
    14161 gaactccatt actgtcaggg taaaagaagg atttgacagc gccgaggcgg aacagcaact
    14221 cacgcgttta ctttcactgc gccacggaaa gaaggatttc tttacctgga acatggacgg
    14281 cgtcttgaaa actgttgaaa agaccacacg tactttacaa ctgtttctga cgctggtggc
    14341 ggtgatttcg ctggtggtgg gcggtattgg tgtaatgaat attatgctgg tgtcagtgac
    14401 cgagcggacg cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt
    14461 gcaacagttc ctgatcgaag ccgtactggt ttgcctggtc ggtggcgcgt tgggaataac
    14521 actgtcactg ttaattgctt tcaccttgca gcttttctta cccggctggg agattggttt
    14581 ttcaccgttg gcactgctgc tggcgtttct ctgctcgacg gtcaccggga ttttatttgg
    14641 atggttaccc gcacgaaatg cggcacgact ggatccagta gatgctctgg cacgagagta
    14701 atttttgaga taaaaatgcc agccgatcgg gctggcattt tgcctttagg atgtacacaa
    14761 tgagacagaa gagctatgcg actgccgctt ctacttcgac gggcacaata acactggcgt
    14821 gattgccttt tggcccctgg tggacatcaa actgaacgga ttgtccagct tttagcgttc
    14881 tgtaaccatc catctgaatg gtggaataat gagcgaaaat atcttcgccg ccgccttcag
    14941 ggcagatgaa accaaaccct ttggcattgt tgaaccactt aacagtaccc ttttccatgc
    15001 ttcgacatcc ttcgcaaatc ttatacaagt aagatggaat aaaccggggt cagagagggg
    15061 gctgttcaaa acctcgccaa ctctagaaat acaatttaga gaattagggc gagccgtcaa
    15121 gcgtttgaca ggggccaggg ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt
    15181 gacaggaatg tgacagatgt cgctgatgcc aacgatagat gatagttatc tatcatgtgg
    15241 agtagattgg tcaggcaaat aagctcttgt cagcggcagg gcgttctacc gataaccgta
    15301 accgaagatg ataactgaca atgggtaaaa cgaacgactg gctggacttt gatcaactgg
    15361 cggaagaaaa agttcgcgac gcgctaaaac cgccatctat gtataaagtg atattagtca
    15421 atgatgatta cactccgatg gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg
    15481 atgtagaacg tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag gccatttgcg
    15541 gagtctttac cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg
    15601 agaatgagca tccattgctg tgtacgctag aaaaagcctg aatgcaggca taaaaattgg
    15661 gggaggtgcc tatgctcaat caagaactgg aactcagttt aaatatggct ttcgccagag
    15721 cgcgcgagca ccgtcatgag tttatgaccg tcgagcactt gttactggcg ctgctcagta
    15781 acccatctgc ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg ctccgtcagg
    15841 aactggaagc ctttattgaa caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg
    15901 acacacagcc gacgctgagt tttcagcgtg tactgcaacg tgcggtcttc catgtccagt
    15961 cctccggtcg caatgaggta accggtgcaa acgttctggt cgctatcttt agcgaacagg
    16021 agtcgcaggc ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat gtggtgaact
    16081 ttatctctca tggcacgcgt aaagacgagc cgacacagtc ttctgatcct ggcagccagc
    16141 caaacagcga agaacaagct ggtggggagg aacgtatgga gaatttcacg acgaacctga
    16201 atcagcttgc gcgcgtgggc ggaatcgacc cactgattgg tcgtgagaag gagctggagc
    16261 gtgctattca ggttctctgc cgtcgccgta aaaacaaccc gctgctggtg ggggaatctg
    16321 gtgtcggtaa aaccgcgatt gcggaaggtc ttgcctggcg aattgttcag ggcgatgtgc
    16381 cggaagtgat ggctgactgt acgatttact ctctcgatat cggttctctg ttagcgggca
    16441 ctaaatatcg cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcagg
    16501 acactaacag catcctgttt attgatgaga tccacaccat tatcggtgcg ggtgcagcgt
    16561 ctggtggcca ggtcgatgcg gctaacctga tcaaaccgtt gctctccagc ggtaaaattc
    16621 gcgtaattgg ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa gaccgtgctc
    16681 tggcgcgtcg cttccagaaa attgatatta ctgaaccgtc gatcgaagaa actgttcaaa
    16741 tcatcaatgg cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat accgcaaaag
    16801 cggtgcgtgc agcggtagag ctggcggtga aatacattaa cgatcgtcat ctgccggata
    16861 aagccattga cgttatcgac gaagcgggcg ctcgcgcacg cctgatgccg gtaagcaaac
    16921 gcaagaaaac cgttaatgtg gcggatattg agtccgtggt ggcccgtatt gcgcgcattc
    16981 cagagaagag tgtttctcag agtgaccgcg ataccctgaa aaacctcggc gatcgcctga
    17041 aaatgctggt cttcggtcag gataaagcca ttgaggcgct gactgaagcc attaagatgg
    17101 cgcgtgcagg tttaggtcac gaacataaac cggttggttc gttcctgttt gccggcccta
    17161 ccggggtcgg gaaaacagag gtgacggtac agctttcgaa agcgttgggc attgagctgc
    17221 tgcgctttga tatgtccgag tatatggaac gccataccgt cagccgtctg attggtgcgc
    17281 ctccgggata cgttggtttt gatcagggag gtttgctgac tgatgcggtc atcaagcatc
    17341 cacatgcggt gctgttgctg gacgaaatcg agaaagcgca tccggacgtg ttcaatattc
    17401 tgttgcaggt gatggacaac ggtacgctga ccgataacaa cggacgcaaa gcggacttcc
    17461 gtaacgtggt gctggtgatg accaccaacg ccggggtacg tgaaactgag cgtaaatcca
    17521 ttggtcttat ccaccaggat aacagcaccg atgcgatgga ggagatcaag aagatcttta
    17581 caccggaatt ccgtaaccgt ctcgacaaca ttatctggtt tgatcatctg tcaaccgacg
    17641 tgatccatca ggtggtggat aaattcatcg tcgagttgca ggttcagctg gatcagaaag
    17701 gtgtttctct ggaagtgagc caggaagcgc gtaactggct ggccgagaaa ggttacgacc
    17761 gggcaatggg cgcacgtccg atggcgcgtg tcatccagga caacctgaaa aaaccgctcg
    17821 ccaacgaact gctgtttggt tcgctggtgg acggcggtca ggtcaccgtc gcgctggata
    17881 aagagaaaaa tgagctgact tacggattcc agagtgcaca aaagcacaag gcggaagcag
    17941 cgcattaatc tgattgttag gtaggttggt caagtccgta atctcgaaag aggttacgga
    18001 ctttttgttt atggggtgga ggaggttcag accctttttt aatgatgatg gtaagttgtt
    18061 gataattagt gctgcgggaa ggtaaggata aaaaagggtg ctgcaggaga atgggatggt
    18121 tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc
    18181 ttagaaatcg acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg tacagacaga
    18241 taaaatgcga aaaaaaagct cgcactttcg tacgagctct tctttaaata tggcggtgag
    18301 ggggggattc gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag
    18361 ccactcggac acctcaccaa attgttttgc taccaaacct catgggtggc aacggggcgc
    18421 tactataggg agttggagta aaacggtcaa gaagaatttt aatgataatt attgtttgct
    18481 catactgtaa acaagttgtg cagtatatct acatcgagac aagttacgga cttatacttc
    18541 caaagtactt catacatatc acaaaataaa aaggccggtt aaaccgacct tttactcgtt
    18601 ctttctcttc gcccatcagg cggtaaaaca atcagcgact acggaagaca atgcggcctt
    18661 tgctcaggtc gtacggggtc agttcaacag tcactttgtc gcccgtcagg atgcggatgt
    18721 agtttttgcg cattttaccg gagatgtgtg cagtaaccac gtgaccgttt tctaactcta
    18781 cgcggaacat ggtattaggc aacgtttcaa gaacggtacc ttgcatttca atattgtctt
    18841 ctttggccat ctaatcctct ggggtatcac taccgtaatt tgaaccggca agataatgcc
    18901 gaagttctgt aaataagtaa agatttgcgc gctaaatcgc aacaaacagg ttcggcacat
    18961 tactccgaaa acacacggct aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga
    19021 taaacgatgg gcgttacctg acgcgaaaaa atccttatcg gcagcggggt aatgagcgta
    19081 accaactctg cgaccgcaat tataacactc tggggagaaa tgtgccgaaa acattcattc
    19141 ttgtggtgaa aacaagcatc gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat
    19201 ttgattgaga taattaaggt aatcccggcg gggaatttcg caggcaccaa gcgatgctgt
    19261 gtgatcgtta aggacctggc agtcgataag cttaccgcca tgaccgataa attcctcaca
    19321 gaataccaga agcgccgttt tagacgcatt ttccatccgg ctgaacatgg actcgccaca
    19381 aaatagcgtt ccctgggcca cgccgtacat accgccgaca agctcatctt cacgccagac
    19441 ttcaatggag tgggcatggc cgagttcgtg aaggcgatgg taggcttcga ccacgccacg
    19501 cgtgatccag gttccttctt cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc
    19561 gtaattcatc gtgacacgat agggcgagcg tttatgaaat cgcttcatac tacggctgat
    19621 atgcagtgat tctggccata gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc
    19681 gcctggagaa aaccacggaa aaataccacg ctggtaagcc attaacaggc gcgcagggct
    19741 aagatcgccc ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg
    19801 gaaggctatt gaatggcgag aaagctgaac caggcgcatg accgcaaaac tccacgcaag
    19861 tcggatcgtt caataatagc ttacaaaccc tgcttgaact ggtaataacg cccctgtctg
    19921 gcaagcagct ctgcgtgagt accttgctca ataatttgcc cgttgtccat cactattatt
    19981 tgttggaaac gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt tttctcacgc
    20041 atcatttctg caagcaattc aaggatctgg ctttcggttg tggcatctaa gccttcggta
    20101 ggttcatcca gcaacaccag tggcgcatca tgtaacagcg cacgggcgat agccagacgg
    20161 cgcagttcac caccggagag ctggcgtccg ccttcaccta accaactgtt gagacctgca
    20221 tcctcgagca gcttctccag accaacgcga cgcaagctct ccgccagagc ctcatcacta
    20281 ctgccaggcg aggcgagtaa cagattatca cgcagcgtgg cgctaaacag atgcactcgc
    20341 tgaggaacaa cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc tatggggcta
    20401 tcgttaagca aaatctctcc ctgttgcggg tcccaagcgc gggtcagcag ttgtaacagt
    20461 gttgatttgc cgcatccggt tcgcccgaga atcgcaatat gttccccggc gtttacctga
    20521 agagaaatcc ctttaagtgc ctgttgggat tgctccggat aagtgaactg aacatcccgt
    20581 aatgtcagcg aaacgcgatc ggcaacacga gtttgggtat cagggaaggt gacttccggt
    20641 ttttgatccg ttaaatcagt gatccgaacg gcagaggcaa tgacttgccc cagatgctga
    20701 aatgcacccg ttactggtgc cagtgcttca aacgcggcta acgcgcagaa gacaaacagg
    20761 gcaattaacg cgccgggttg agcattgccg ccaacgccgc cagacgccat ccacagcatc
    20821 aggatcaccg ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc ggtcagttca
    20881 gattgacggc gttgcgcttc cagccattga atttctgtat tctctagttg cgtgcgataa
    20941 cgatcgctgg cgccaaaaat ggtcagctca gcttgccctt gcagccaggc cgtcagttgt
    21001 tggcgatact gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc cgcacgataa
    21061 aacagcggtg gcatcaggaa aagcgtcagt aacataatgc cgcccagcct aaaggcgagg
    21121 gtgaaatcaa ggaaacttaa cccgattgtc accaccataa tcaccacaaa agcgcccacc
    21181 agcggcgaga taacgcgcag ataaagatga tcgagcgtat caacatccgc caccacgcga
    21241 ttgagcaatt cgccctgacg atagcgcgcc agtccggcag gggagagggg cagcaatttg
    21301 ctgaaggtgt aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga
    21361 cgttcaaaat aacgcccggc agtacgggtg attgctgcgc cacgcacgcc cgcagcgggt
    21421 agcatatagt tgaagctgta cagtccggca acccccgcaa ccgctgaggc cgagaggaac
    21481 cagccggaaa gtgtcaacag accgatactg gcgagcagcg tgacaattgc cagcacgata
    21541 ccaagactta acatccattt atgacgttta tacagtgcca gatagggtag caaagcgcgc
    21601 atttaaatct cctcctgacg atgggccagt aatgtggcga atgggccacc agccacactt
    21661 aattccgcgt aacgtccttg ctcaataatc cggccatcct gcataaccca aatgacatcc
    21721 cagtcagcaa gatcttctaa ctggtgggtg accattaacg ttgtctggcg cagagaggcg
    21781 gcattcagcg cctccattac gcgctgttca ctgtgagcat caaggctggc agcgggttca
    21841 tccaacagta atagcgaaca gggatttagt aacgcacggg ccaccgccac gcgctgcgcc
    21901 tgccccacgg aaaggcgggc agcctggtcg ccaacaggtg tatcaacgcc ttgtggcagg
    21961 agcggtagaa actcgctgac ccaggcgtta tccagcgctg cttgtaattc ttgttcgctg
    22021 gcatcaggtc gcgccagtag tacgttatcc cgcaatgttg ctgccggtaa ttgtgggttt
    22081 tgcccaaccc aggagagatg tttacgccat gattctggtg ataaatcgcg taattctatc
    22141 ccgttgattc gtaatgatcc ctgatatgag agaaaaccag aaagcgcgtt cagcagtgag
    22201 cttttacctg aaccgctgcg accaaccaac actgcacgtt ggcctgctgg caaagtaaag
    22261 ttcagcggtc cggccagcgt tttaccttcc ggcgacgtga taaacagatc ctcggcctca
    22321 atggtcaccg gatcggtcga tgctaattcc acctcgccac gctgcggatg ggcgagcggg
    22381 gtttccataa acgttttcag actgtcagct gcaccaacag cctgggcttt agcatgataa
    22441 aacgtaccga gatcgcgcaa tggctggaaa aactctggcg caaggatcag ggccagaaaa
    22501 cccgcagcca gcgtcacacc ggtatcgtag tgaccaaaat ccagctcgcc gagataggaa
    22561 aaaccaaagt agaccgccac cagagcaatt gacagcgagg taaaaaattc gagaatgccg
    22621 gaggataaaa acgccagccg tagcacttcc attgtccgtt ggcggaaatc ttccgaagca
    22681 gaacgaatac tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg
    22741 cgcaggcgat cgaggaaatg cccacttaag cgagcaagag cgagaaagtt acgccggtta
    22801 gcatcggcag cccccatccc aaccagcgcc ataaacaacg gaattaacgg tgctgtgccc
    22861 agcagaatga gcgccgcagc ccagttagag gggaagatcg ccaccacaat cagcaacggc
    22921 accgacactg ccagcgccat ttgcggcagg tagcgtgcat agtaatcatg catatcgtca
    22981 atttgctcga gtaccaacgt cgcccagctc cccgcaggtt taccctgaat ccacgctggc
    23041 cctgcttgtt gcagacggtc gagaacctga cggcggatgg caaagcggat atgctgcccg
    23101 gcgtgataac ccacccgttc gcgtaaccag accacccatg cgcgcagtac aaaggtcaga
    23161 accagtaacg taaagggaag cagcagggct tcacggggaa tattctccat aatcatatgt
    23221 tgcagaattc gcgccatgaa ccaggcctgg gcaatgatca atatgccgct tacaaagccc
    23281 agcagacgag aaatattcag ccaacgttgg gagatgacgc tttgctgttt taaccagcgg
    23341 gttaactctt tttgacggga tttattcatt gcacgcttag caggtgagtt atcagaatta
    23401 tttgcagagc aatgttacaa cggggaaaaa ataaaggcga cccatagtcg catggtgtcg
    23461 ccttctttac ttttgttact gatttgtaaa attattttgc gtcagctaaa ccatcgaggt
    23521 agcgttccgc atcaagtgct gccatgcagc ctgtaccggc cgaagtaatg gcctgacgat
    23581 aaatatgatc catcacgtca cccgcggcaa agacgccagg aatgctggtc tgggtggcat
    23641 taccatgaat acccgactgc actttgatgt agccgttttc cagttccagc tgcccttcga
    23701 aaatggcagt attcgggctg tgaccgatag caacaaacag accagcaacg tcgagtgact
    23761 cgatgttatc gctgttttgc gtatcgcgca gacgaacgcc agtgacaccc atttgatcgc
    23821 cggtcacttc ttccagcgta cggttggtgt gcagaatgat gttgccgttc tccactttat
    23881 ccatcagacg cttaataagg attttttccg cgcggaaacc gtcacggcgg tgaataagat
    23941 gcacttccga agcgatgtta gacagataca gcgcctcttc aaccgcggta ttgccgccgc
    24001 cgatgaccgc aactttctgg ttgcgataga agaaaccgtc gcaggttgca caagcagaaa
    24061 ccccactgcc tttaaaggct tcttcagagg gcaggccgag atagcgtgca gaagctccgg
    24121 tggcaataat cagcgcgtcg caagtgtatt cgccgttatc gccattcaga cggaacggac
    24181 ggttttgcag atccaccttg ttgatatgat caaaaatgat ctcagtttca aacttggtgg
    24241 catgttcgtg catgcgctcc attaataacg gaccggtcag atcgtttgga tcgccaggcc
    24301 agttttccac ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg gtaatcagca
    24361 caggttgcag gttggcgcgc gccgcgtaga cagcagcggt gtatcccgcc gggcctgaac
    24421 ccaggataag cagtttactg tgtttggtcg tgcccatgag atccccatag ttgttggcag
    24481 acaatgggca ggattgtagg gaatttacag acgtaaaaaa agagtatgac gattttgtta
    24541 acaatttgtg caatcggcag catcgataag caggtcaaat tctcccgtca ttatcacctc
    24601 tgctacttaa atttcccgct ttataagccg attaaatgat gaataaacgc ccctgttaat
    24661 gaatatctgg catgttgtac taaaaatcga tgttttgctt tgacaatccc ctggtgtttt
    24721 gcgaaaacat tcgaggaaga aaaaaacagt attcttatat gcgcataacc atgcatgtaa
    24781 ataccatgtt taccgtgcta gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat
    24841 gtcgatagct gccacaaggc aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg
    24901 aatcttcatg atttcggtct atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg
    24961 tcagacagga gtagggaagg aatacagaga gacaataata atggtagata gcaagaagcg
    25021 ccctggcaaa gatctcgacc gtatcgatcg taacattctt aatgagttgc aaaaggatgg
    25081 gcgtatttct aacgtcgagc tttctaaacg tgtgggactt tccccaacgc cgtgccttga
    25141 gcgtgtgcgt cggctggaaa gacaagggtt tattcagggc tatacggcgc tgctgaaccc
    25201 ccattatctg gatgcatcac ttctggtatt cgttgagatt actctgaatc gtggcgcacc
    25261 ggatgtgttt gaacaattca ataccgctgt acaaaaactt gaagaaattc aggagtgtca
    25321 tttagtatct ggtgatttcg actacctgtt gaaaacacgc gtgccggata tgtcagctta
    25381 ccgtaagttg ctgggggaaa ccctgctgcg tctgcctggc gtcaatgaca cacggacata
    25441 cgttgttatg gaagaagtca agcagagtaa tcgtctggtt attaagacgc gctaacacgg
    25501 aacaggtgca aaatcggcgt attttgatta cactcctgtt aatccataca gcaacagtac
    25561 tggggtaacc tggtactgtt gtccgtttta gcatcgggca ggaaaagcct gtaacctgga
    25621 gagcctttct tgagccagga atacactgaa gacaaagaag tcacattgac aaagttaagt
    25681 agcggccgcc gccttctgga ggcgttgctg atccttattg tcctgtttgc cgtctggttg
    25741 atggctgcct tactaagctt taacccttcg gaccccagct ggtcgcaaac ggcctggcat
    25801 gaacctatcc ataatttagg tgggatgccc ggtgcgtggt tggcagatac gctgttcttt
    25861 atttttggcg tgatggctta caccattccc gtcattattg tcggcggttg ttggtttgcc
    25921 tggcgtcatc agtccagcga cgaatacatt gattattttg ccgtttcgct acgcatcatt
    25981 ggcgttttgg cgctcatcct tacctcctgt ggtctggcgg caatcaacgc tgacgatatc
    26041 tggtattttg cctccggtgg cgtcattggc agcttactaa gtactacgct acaaccactg
    26101 ctacacagta gcgggggaac tattgcgctg ctctgcgttt gggctgcggg cctgacgctg
    26161 ttcaccggtt ggtcatgggt gaccattgct gaaaaactcg gcggctggat tttaaacatt
    26221 ctcaccttcg ccagtaatcg tacccgtcgc gatgatacct gggtcgatga agatgaatat
    26281 gaagatgacg aagagtatga agatgaaaac cacggcaaac agcatgaatc acgccgtgcc
    26341 cgtattcttc gcggcgcgct agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg
    26401 atggggcggc aaacagacgc tgcgttgttc tccggtaagc ggatggatga tgacgaagag
    26461 attacctaca ctgcacgcgg tgtggctgcc gacccggacg acgtcctatt ttcgggcaat
    26521 cgtgcaacgc agccagaata tgacgaatac gatccattat taaacggtgc gccaattacc
    26581 gaacctgtcg ctgtagcagc tgctgctacc acggcgacac aaagctgggc tgcgccggtt
    26641 gaacctgtga ctcagacgcc gcctgttgcc tctgttgatg ttccaccctc gcaacctaca
    26701 gtagcctggc agcctgtacc gggtccacaa acgggagagc cggttattgc tcctgcaccg
    26761 gaaggttacc cacagcagtc acaatatgcg cagcctgcag tgcaatataa tgagccgctg
    26821 caacaaccag tacagccgca gcagtcgtat tatgcacctg cagctgaaca acctgcgcaa
    26881 caaccttact acgctcctgc ggcggaacaa cctgttcagc aaccgtatta cgcaactgcg
    26941 ccggaacagc ctgcacaaca gccgtattac gcccctgcgc cagaacaacc ggtggcaggt
    27001 aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact
    27061 gagcaaactt atcagcaacc agccgctcag gagccgttgt accaacagcc gcaatccgtt
    27121 gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg
    27181 ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg
    27241 gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgttg
    27301 aaagcgcctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg
    27361 gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca
    27421 gtcttcagtc tggcaaatag cggtggaccg cgtccgcaag tcaaagaggg gattggcccg
    27481 cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaactggc gtcttacggt
    27541 attaagctgc cctcacagcg tgcggcggaa gagaaagccc gtgaagccca gcgcaatcag
    27601 tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca ggatgaactg
    27661 gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgtg
    27721 cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg
    27781 caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg
    27841 gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgttg
    27901 tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc gcagcagcag
    27961 tatcagcagc cgcaacaacc agttccgcca cagcagcaat atcagcagcc gcaacagccg
    28021 gttgcgccgc agccgcagta tcagcagcca caacagcagg ttgcgccgca gccacaatat
    28081 cagcagccgc aacaaccggt tgcgccacag ccgcaatatc agcagccgca acaaccagtt
    28141 gctccgcagc cgcagtatca acagccgcaa caaccggttg caccgcagca gcaggatacc
    28201 ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcataa accgacgacg
    28261 ccgctgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc
    28321 tttgcgcttg aacaaatggc tcgcctggtg gaagcgcgtc tggctgattt ccgtattaaa
    28381 gccgatgtcg tcaattactc tccggggccg gttatcactc gctttgaact taacctggct
    28441 cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg
    28501 acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta
    28561 ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc
    28621 gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt
    28681 gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct
    28741 gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt
    28801 ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcatctg
    28861 ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac
    28921 gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat
    28981 aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag
    29041 ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtggtg
    29101 ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata
    29161 gcacgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt
    29221 ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt
    29281 accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg
    29341 ctggggatgg gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat
    29401 ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt
    29461 cgcccacagt atgttgatgg catcacttcc gacagtgaaa gcgaaggtgg cgcgggcggt
    29521 ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa
    29581 aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca
    29641 gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt
    29701 aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg gataaggcgc
    29761 ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag
    29821 ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg
    29881 aggaataatg atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag
    29941 cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt
    30001 ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca
    30061 gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga
    30121 aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc
    30181 tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa
    30241 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac
    30301 gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac
    30361 aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc aactgaaatc
    30421 ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac
    30481 ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg
    30541 ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg
    30601 gccagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatt
    30661 tacattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga
    30721 ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag
    30781 agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc
    30841 tttttgttga cgaagtgcac cgattcaaca aaagccagca ggatgcattt ctgccacata
    30901 ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta
    30961 attcggcact gctttcccgt gcccgtgtct atctgttgaa atccctgggt acagaggata
    31021 ttgagcaagt actaactcag gcgatggaag acaagacccg tggttatggt ggtcaggata
    31081 ttgttctgcc agatgaaaca cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc
    31141 gggcgttaaa tacgctggaa atgatggcgg atatggccga agtcaatgat agcggtaagc
    31201 gggtcctgaa gcctgaatta ttgaccgaaa tcgccggtga gcgaagcgcc cgctttgata
    31261 acaaaggcga tcgcttttac gatctcattt ccgcactgca taagtcggta cgtggtagcg
    31321 cacccgatgc ggcgctctac tggtatgcgc gaattattac cgctggtggc gatccgttat
    31381 atgtcgcgcg tcgttgcctg gcaattgctt ccgaagatgt cggcaatgcc gacccacggg
    31441 cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg
    31501 aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct
    31561 acactgcgtt taaagctgcg ctggcggatg ctcgcgaacg tccggattat gacgtgccgg
    31621 ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc
    31681 gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa
    31741 tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa
    31801 agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt
    31861 atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca
    31921 tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag
    31981 acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg
    32041 ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta
    32101 actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac
    32161 gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt
    32221 tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc
    32281 cggtaggtaa agacgaaaat gacaacgtcg aagtcagccg ctggggcacc ccgcgtgagt
    32341 ttgactttga agttcgtgac catgtgacgc tgggtgaaat gcactctggc ctcgactttg
    32401 cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca
    32461 tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg
    32521 agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga
    32581 aatttgctgg cgatctgttt catactcgtc cgctggaaga agaagcagac accagtaact
    32641 atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg
    32701 atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg
    32761 gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa
    32821 tggtgcagat cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg
    32881 cagaaaaagt cctgcagttg ttgggcctgc cgtaccgtaa aatcatcctt tgcactggcg
    32941 acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca
    33001 cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcgcgt cgtatgcagg
    33061 cacgctgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg
    33121 gactggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc
    33181 gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat
    33241 acccaatttt tctgaatcta aaaagcgcct gcgggcgctt taagtagaag tttaattatt
    33301 tgaaaataaa tggattttat ttttaagtgt ccatataacg tccatctttg aatgcaaaaa
    33361 gcccacagca ttctatgcta tgggctttgt ttttggtgtc ctcatgagtg aggg
//
LOCUS       DAZFKM010000041        33356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFKM010000041 DAZFKM010000000
VERSION     DAZFKM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(44..1903)
                     /gene="gss"
                     /locus_tag="ACHKAY_002058"
     CDS             complement(44..1903)
                     /gene="gss"
                     /locus_tag="ACHKAY_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO5700737.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2108..2974
                     /gene="yghU"
                     /locus_tag="ACHKAY_002059"
     CDS             2108..2974
                     /gene="yghU"
                     /locus_tag="ACHKAY_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO5700738.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3098..3346)
                     /gene="hybG"
                     /locus_tag="ACHKAY_002060"
     CDS             complement(3098..3346)
                     /gene="hybG"
                     /locus_tag="ACHKAY_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO5700739.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3359..3700)
                     /gene="hypA"
                     /locus_tag="ACHKAY_002061"
     CDS             complement(3359..3700)
                     /gene="hypA"
                     /locus_tag="ACHKAY_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO5700740.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3693..4181)
                     /gene="hybE"
                     /locus_tag="ACHKAY_002062"
     CDS             complement(3693..4181)
                     /gene="hybE"
                     /locus_tag="ACHKAY_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO5700741.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4174..4668)
                     /locus_tag="ACHKAY_002063"
     CDS             complement(4174..4668)
                     /locus_tag="ACHKAY_002063"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO5700742.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4668..6371)
                     /gene="hybC"
                     /locus_tag="ACHKAY_002064"
     CDS             complement(4668..6371)
                     /gene="hybC"
                     /locus_tag="ACHKAY_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO5700743.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6368..7546)
                     /gene="hybB"
                     /locus_tag="ACHKAY_002065"
     CDS             complement(6368..7546)
                     /gene="hybB"
                     /locus_tag="ACHKAY_002065"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO5700744.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7536..8522)
                     /gene="hybA"
                     /locus_tag="ACHKAY_002066"
     CDS             complement(7536..8522)
                     /gene="hybA"
                     /locus_tag="ACHKAY_002066"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO5700745.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8525..9643)
                     /gene="hybO"
                     /locus_tag="ACHKAY_002067"
     CDS             complement(8525..9643)
                     /gene="hybO"
                     /locus_tag="ACHKAY_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO5700746.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9832..10119)
                     /gene="yghW"
                     /locus_tag="ACHKAY_002068"
     CDS             complement(9832..10119)
                     /gene="yghW"
                     /locus_tag="ACHKAY_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO5700747.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10238..11125)
                     /gene="yghX"
                     /locus_tag="ACHKAY_002069"
     CDS             complement(10238..11125)
                     /gene="yghX"
                     /locus_tag="ACHKAY_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO5700748.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11282..12322
                     /gene="gpr"
                     /locus_tag="ACHKAY_002070"
     CDS             11282..12322
                     /gene="gpr"
                     /locus_tag="ACHKAY_002070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO5700749.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12362..12856)
                     /locus_tag="ACHKAY_002071"
     CDS             complement(12362..12856)
                     /locus_tag="ACHKAY_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO5700750.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13047..13931
                     /gene="yghA"
                     /locus_tag="ACHKAY_002072"
     CDS             13047..13931
                     /gene="yghA"
                     /locus_tag="ACHKAY_002072"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5700751.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14203..14628)
                     /gene="exbD"
                     /locus_tag="ACHKAY_002073"
     CDS             complement(14203..14628)
                     /gene="exbD"
                     /locus_tag="ACHKAY_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO5700752.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14635..15369)
                     /gene="exbB"
                     /locus_tag="ACHKAY_002074"
     CDS             complement(14635..15369)
                     /gene="exbB"
                     /locus_tag="ACHKAY_002074"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5700753.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15621..16808
                     /gene="metC"
                     /locus_tag="ACHKAY_002075"
     CDS             15621..16808
                     /gene="metC"
                     /locus_tag="ACHKAY_002075"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO5700754.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16948..17607
                     /gene="yghB"
                     /locus_tag="ACHKAY_002076"
     CDS             16948..17607
                     /gene="yghB"
                     /locus_tag="ACHKAY_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO5700755.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17647..18546)
                     /gene="yqhC"
                     /locus_tag="ACHKAY_002077"
     CDS             complement(17647..18546)
                     /gene="yqhC"
                     /locus_tag="ACHKAY_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO5700756.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18740..19903
                     /gene="yqhD"
                     /locus_tag="ACHKAY_002078"
     CDS             18740..19903
                     /gene="yqhD"
                     /locus_tag="ACHKAY_002078"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO5700757.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20008..20835
                     /gene="dkgA"
                     /locus_tag="ACHKAY_002079"
     CDS             20008..20835
                     /gene="dkgA"
                     /locus_tag="ACHKAY_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO5700758.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21035..21961
                     /locus_tag="ACHKAY_002080"
     CDS             21035..21961
                     /locus_tag="ACHKAY_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO5700759.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22012..22269
                     /gene="yqhH"
                     /locus_tag="ACHKAY_002081"
     CDS             22012..22269
                     /gene="yqhH"
                     /locus_tag="ACHKAY_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO5700760.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22312..24531)
                     /locus_tag="ACHKAY_002082"
     CDS             complement(22312..24531)
                     /locus_tag="ACHKAY_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO5700761.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24642..26054)
                     /gene="ftsP"
                     /locus_tag="ACHKAY_002083"
     CDS             complement(24642..26054)
                     /gene="ftsP"
                     /locus_tag="ACHKAY_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO5700762.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26129..26866)
                     /gene="plsC"
                     /locus_tag="ACHKAY_002084"
                     /gene_synonym="parF"
     CDS             complement(26129..26866)
                     /gene="plsC"
                     /locus_tag="ACHKAY_002084"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO5700763.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27099..29357)
                     /gene="parC"
                     /locus_tag="ACHKAY_002085"
     CDS             complement(27099..29357)
                     /gene="parC"
                     /locus_tag="ACHKAY_002085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO5700764.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29903..30385)
                     /locus_tag="ACHKAY_002086"
     CDS             complement(29903..30385)
                     /locus_tag="ACHKAY_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO5700765.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30438..30830)
                     /gene="ygiW"
                     /locus_tag="ACHKAY_002087"
     CDS             complement(30438..30830)
                     /gene="ygiW"
                     /locus_tag="ACHKAY_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO5700766.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30982..31641
                     /gene="qseB"
                     /locus_tag="ACHKAY_002088"
     CDS             30982..31641
                     /gene="qseB"
                     /locus_tag="ACHKAY_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO5700767.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31638..32987
                     /gene="qseC"
                     /locus_tag="ACHKAY_002089"
     CDS             31638..32987
                     /gene="qseC"
                     /locus_tag="ACHKAY_002089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO5700768.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33033..>33356)
                     /locus_tag="ACHKAY_002090"
     CDS             complement(33033..>33356)
                     /locus_tag="ACHKAY_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO5700769.1"
                     /translation="HKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICSIPS
                     VSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGR
                     FIFCY"
BASE COUNT         8204 a         8912 c         8417 g         7823 t
ORIGIN      
        1 ctaattattc ttcataatca gtacggggtt gctacaacat gtattacttt ttcaccacaa
       61 ttaacggttc aatatcactc tcttttttga tgaccagtga ttcatcaccg cgcaaacacg
      121 tcccaccgta gttgccgcca acggtgaagg tacatacctg aatgtattta ccgtccactt
      181 tcggcaaaca ccacagttgc tgatagatgt ttttctgctc ggcaaattta ccgctggttt
      241 tgtccagcac ctcttcatga tggctgacca ggttgatatt gctaccacag cgaccggcaa
      301 tcggtttcac tgcgtaacct gttttcacca attcatcatt aacagtgaaa tcggtatcca
      361 gcaggtaacg atggtgcggg aacagcgacc agaggatcgg cagaatcgct ttgttgccgg
      421 ggatcaccgt ccacagcggc tcaaagacta gcacttccgg gcgcagcaat acgtcgataa
      481 gacgcacttc gttttgcgga tgaccggtac ggattggcac cgcagcaaac tcacggtcgc
      541 taacttcacg aatctgatca aacgcggttt cccacgccca ggttttccac acgcagttaa
      601 ccagccgtcc ttccccatca atcagttgcc cggcagcatc ccagcccagt tcatccagcc
      661 cgcgcaagat acgcgtttca aagccctcct ggtgcagcgc ctgctccata aactgcgcgt
      721 gatagttttc ctcgatatct ttgtcctgca tgatatggac aaacggacgt gcacgactgt
      781 gtttccaggc accagccagt tcgttaatca gcccttccgc cggattgaag ccgttgcctt
      841 tatagccctg ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga catgaggcag
      901 agtcggcgtt gtattcgtaa accttcaggc cacgctcatc catgcagaaa tccatacgac
      961 cagtgatcat atggtgacgg cgacgctgcc aggagagacg caaacgtggc cagaggattt
     1021 tcgggatgtc gaacagcgcc agcaggttgt catctttcag caccttgtcg gttgcgtgaa
     1081 gatacatcag gtgcagctcg ttggtggctt taattagctc ctgctcggca ctctcggtaa
     1141 tggtgtagta atgataagga tcctgattga tcacctgacc gttggcctgc acataggcgt
     1201 tttgcagcgg atctttttca tccagccatt taccgtcaaa ctggcctttg ttttccaggc
     1261 gcgctccgct gattttcagc agctcgcctg caatttccgg ctgcggtaag ctgtattcag
     1321 tatcttccgt ctggatcatc cagcccagaa tggtggtgtc atcaaaagtg tctttcaggg
     1381 tatagcagcc gttttcgacc accatctcca gctcacgcgt ccactgttgc ccttgcggca
     1441 acggggaatg aatcacgttc tgttccgcaa tacggacttt gttgccatgc aattgggtaa
     1501 tgatggcgac atggccagtg tctttaaatt caccgccttt atcccagata agaagcgcac
     1561 ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg caatggcagg atgttgtcat
     1621 taaccacttc tcgcaggaag cgcagcgaga aaatctccca cgccataccc acgtcagtaa
     1681 agaccacacc gtaattcaga aagagaaaac ggcgagcaaa ttcaacgcat tgccacttgt
     1741 ggcccatata ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg tattcctgcg
     1801 gatcgagaga actgtaatct gaagagtaga ttgctacccc acctggggcg tagcccaata
     1861 atgtcccgaa cggggcatcc tggctggtcg ttcctttgct catcacttta cctttaacaa
     1921 tacaacctaa gcagttggcg taattgttgt gctctgatta cctgctcagc gacattaacc
     1981 ggacagaggt cagactaatc atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa
     2041 ttcacattct gtacagcaag tgacctgcta cactgcttca acactaccac tcagaaggca
     2101 actcactatg acagacaata cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc
     2161 tggcggcgcg ttcgccaata tcaatcgccc ggtttctggt ccgacgcatg aaaaaacgct
     2221 gcccgttggc aaacacccat tgcaacttta ttcgctggga acgccgaacg gtcagaaagt
     2281 aacgattatg cttgaggagc tgctggcgct gggcgttact ggtgcagagt acgacgcctg
     2341 gctgattcgt attggcgatg gcgatcaatt ctccagcggc tttgtcgaag tgaacccaaa
     2401 ctcgaagatc ccggcgctgc gcgatcatac gcataatccg ccgatccgcg tgtttgaatc
     2461 tggttcgatc ctgctttatc tggcggagaa atttggctac ttcctgccgc aggatttggc
     2521 aaagcgtact gaaacgatga actggctgtt ctggttacag ggcgcggcac cgttcctcgg
     2581 cggtggtttt ggtcactttt accattacgc gccgatgaaa attgagtacg ccatcaaccg
     2641 ctttaccatg gaagccaaac gtctgctcga cgtgctggat aagcaactgg cgcagcataa
     2701 gtttattgcg ggcgatgagt acaccattgc ggatatggcg atttggccgt ggtttggcaa
     2761 tgtggtgtta ggcggcgtgt atgatgccgc agagtttctt gatgcgggca gttataagca
     2821 tgtacaacgc tgggcgaaag aagtaggcga acgtccggcg gtgaaacgtg ggcgtattgt
     2881 taaccgcacc aacggaccgc tgaatgagca gttgcatgag cgccatgacg ccagtgattt
     2941 cgagacgaat acggaagata agcgtcaggg gtaagggttg gtgttcgtcg cagcaagcca
     3001 tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg tgcacaatgc ctgatgcgac
     3061 gctgccgcgt cttatcagta tctacgaaag ctaatcatca cgcgctggta atgtcgtaat
     3121 ccatttggcg cagtgcgtct aatgtggctt tggcttcatc ttcgtcgatg atgctcatgg
     3181 caaatccgac gtgtaccagc acccactggc ccagtagatc ggcagggtta ccttcacaaa
     3241 tcagggcgat attcacgtcg cgcttgatac cacatacttc gacctgcgca agctggtgaa
     3301 tatcttcacc gacagccagc acctggcctg gaacgccaat acacattatt aactccggtt
     3361 attcaacttc aatacttttg acgatcagcg aatcgccggt atcgacacgc aaccgctcgc
     3421 cgtgacagag cggacactgc gcatcgtgct gatgaatctc caccacctgg ctgcaatccc
     3481 agcaccaagc ctgggcgggt ttatagacga tatgtaaatc gcacccttgc gccaccgttc
     3541 cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc ctcaacgcag gagagcgcgc
     3601 caatttccag ccacacggcg gtgacgcgct taacatcgtg ctgctccgcc tgccgttgga
     3661 taatttcaac ggcgctctgg caaagagaca actcatgcat tttcgccact cctgcgacca
     3721 aacagcaggg cgcgacgccc tgcgtgtggt acatccggat tcgtgactga cagcgaaagg
     3781 atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt caatcgacat gctgtgcgaa
     3841 agcggcgaca tcagcgaaca ggagagatat tgcgaaacac cgtccagttc gccaacggta
     3901 aaggtcatag tgccatacgg cagttgcaga ccaatttttt cactcacttt gcgcagcggc
     3961 cagagttgat ccgggccggg gaagataact gcactcagca tccacggggt gatcacacac
     4021 cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat acaccggcat tgaaggatgc
     4081 agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt caaacgctgc ctgtacttgc
     4141 gccttcgggg aggtctggaa acctgctatc tcttcagtca tgaatcgcct cccgtgggat
     4201 ggcttccacg ccagattcac gcagcgcagc cagaacctgc tcaagcgcag gttcaatcat
     4261 tgcttcaacc gtcggcgtta agccgatgtg tggctccagc gattccggga tcacgccgac
     4321 cagggtcagc tttttcggaa actcaccggt gaagcgcagg gccgacaata cgtcggccag
     4381 gccaagctga tgcggagaga ttttgttggt aaacaacgcc ggaacttctt catcccgcag
     4441 gatcatcatc gttcccggcg tgttcttttt cgacacaatg gcatccgcaa taatcagatg
     4501 atcgcgattt gccatgtcgc caagcagctc cattcccgcc gtgccgccat cgaggatctc
     4561 aacataatcc ggcagaatgt atcgttgctc taacgcttcg acaatccgca caccgatggc
     4621 ttcatcggtc agcaaaatat tgccgacccc taagactaaa atacgcatta cagaaccttc
     4681 actgaaacca cttcgttgcc gtcggcatcc actacgtgta ccgcacaggc catgcacggg
     4741 tcgaaggagt gaatggtacg caccacttcc agcggtttat tcggatcagc aaccggtgta
     4801 cccaccagcg actgctcgta aggaccgacg tcatcattga agttacgcgg accagagttc
     4861 caggttgatg gaacaactgc ctggtagttg ctgatgatac cgtctttaat caccatccag
     4921 tgagagagca taccgcgcgg cgcttcgagg aagccaacac ctttgaactc acccgttgcc
     4981 ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc cgatattggt gatcagtgca
     5041 ctgtattggt tttgcaggat atcctgcaat tcacagcagt gaacggtacg accaataata
     5101 cggcccagcg tggagtgcag ctgtgccact tccagcgtgt tgccagtcag tttctgataa
     5161 atcgcaacga tttcattcag tttgttctgg gtagattctc gacctgccgc cagtttcacc
     5221 agcatattag ccagcggacc cacttctacc gttttgccgt agaaagtcgg tgatttcacc
     5281 caggaatatt tcccgtcgtc agaccaacca tcataagccg gaatggtggt accttcccac
     5341 ggcgcctgtg gcgcttcgtc tttataccag gagtgcttcg cgctttcctg aatgcctttg
     5401 atcaggtatt catcggaatg agaagtgatc ggacgatacg aggacagatc cgcattctca
     5461 atgtagccgc ccgggaacag gaagctgccg tttttgctgt cggtcgggaa ttccggcacg
     5521 ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt ccgggtagaa cgcggcgata
     5581 accgcggtat caaccttata aacctgctca acaaagtcgc tcagtttgtc gatgaaagac
     5641 ttgatgtaca tcaggcgctc aaggttcaac acgcccaacc cgtcgaggtt gattgggttc
     5701 gcgacaccac ccactgccag gttctgaatg tgcggcgttt taccgcccag cagcgccacg
     5761 acgcggttag cgtcacgctg gcactccagc gcctgcaggt agtgcgctac cgcaatcagg
     5821 ttcacttccg gcggcagttt catcgctggg tgaccccagt agccattagc gaaaataccc
     5881 aactgaccgc tggcaaccag atctttgatc ttgttctgaa ctttagtgaa ctcttccgga
     5941 ctgttcagat gccaggtcga aacgcctttc agcatttcgg aggctttggt tgggtcagct
     6001 tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa gctgatagaa atgaacgata
     6061 tggtcatgcg tggtgtgcgc agccagaatg atgttacgga tgtattgcgc gttaaccgga
     6121 acgtcgatat tcagcgcact ttctgccgca cgaacggaag acagcgcgtg agtggtagta
     6181 catacgccac agatacgttg cacaatcatc catgcatcgc gcggatcgcg gtttttcacg
     6241 atctcttcca tgccgcgcca catggtaccg gaagcccatg ctttcgaaac gacgccattt
     6301 tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg ttaccggatc aatagtaatt
     6361 ctctggctca tgctttgctc gcctcatgac gattatgatc gttttgtttt aaaggaggaa
     6421 gtatcggcag tagacgaatg agtacgatgt aagcgcaaat ctcaatagcc acaaaaccaa
     6481 tagaaatcaa cagttcttcc caggtcggga agtaggcgta accgccgccc gggttgaatg
     6541 ccaccagcga ataggtcaga cgccaggttg cacaacctaa cagtgcgctc agtgctgaca
     6601 ggaacagcat gcgggaatca ttacgcagct tcgccacacg cagaaccacc agcgggaaga
     6661 gcatcagcag gacttcaatc cagaacatca cggagtagaa gtcaccggca aacgctaacg
     6721 acagcttgtc gcgatagata agctcgccaa agcgcagcac gatgaaaatc gccagcaaca
     6781 cactgatggt gttggtcagc ttgacgaaca gactcttttc atccggaccg ttgccacgca
     6841 gacccgcctg caccagcgaa ccttcaaaga tgacaatcga gaagcccatg atgaacgccg
     6901 tcagcagcga gaacagcggc aacatttcat agctctgcca caacggatgc accttgtagc
     6961 ccgccgagat catcagtgac cccattgaag actggtgcat ggtcggcaac agcgcgccaa
     7021 gtgcaatgat gaagaacatc accttgttca gacgcttgag agaaaccttc cagcccagac
     7081 gttcaaacag tgccggagca aactccagtg ccatcacgcc gatatagatg gtcatacaga
     7141 ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc cggaatgtag aagtacggca
     7201 ggttccagta gcgacccacg tcgatagtga tcgacaagcc ccccagtgag taaccaaaca
     7261 gactcgccaa cagcgccgga cgcaccagcg gatggtattg cccacggtta aagacgtata
     7321 ccgcccacgc cagcgcccag ccgccacagg caaagccggt accaatcagc aggtcaaacg
     7381 cgatccacac gccccacggg aagccgccgt tcaggtcaga gacagagccc agaccgaaca
     7441 ccagacgctt cacaatcagg agcatacaga tgacgattaa cggtccaaaa atcatgaccg
     7501 gtttactgat gattttgccg cccagcggtt gtggatcatg actcatgatc gtctcctccg
     7561 tcgtgatggt cgtttttggt gttgcgacga accagcacgg ttaagcccgc cagcacagcc
     7621 agtggtagca tcatgccttt atacagggtg tgttgaatat tttcggaacg cgcaccggta
     7681 gaaagatcgt ccagtttcgg caggtcgaga ttttcataag gcacacccgt cagtaccaga
     7741 acctgagtac cgccgccctc tttctcgccg tacagatgcg gataatattt cggcaccgta
     7801 tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat agtggtattc gctgccaggc
     7861 ttcagcgcca gacgtttttt cgcctccgcc atcagctctt cacgcgtacc aaaaattacc
     7921 gcgcccgccg ggcacacttc tacgcagcca ggcaaaccgc ctttatcgag acgttccaca
     7981 cctttctggt tgcacaactc gcacttatgc agcgcaccaa acgggttgtt gtagtcgtac
     8041 ttcggcacgt tgtatgggca ggcgaccatg cagtaacggc aaccggtgca cacatctttg
     8101 tcgtaatgga caatgccggt tttcggatct tttttcagcg cagagaccgg gcacacagag
     8161 acacagttcg gatcgacgca gtgcatacac tgtttcttaa tgtacgcgta gccgttctcc
     8221 tcctggtctt tgttgacccc tgtgccgctg gtccacacct gaatgatgtt attggtatac
     8281 ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt gcgggttacg ttcagggaag
     8341 ttgatatcct gacacttggt gacgcaagcc tggcagccta cgcacaaggt cgagtcgtac
     8401 aacatcccca gcgatcccgg aattggcggg cggttttcag cagccgcatg actgacagac
     8461 ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt taataaaatt acgtctgttc
     8521 acggttattc tccccgtgag tcagcgttat ctttcttttg ctgacgaccc agttcacgca
     8581 ccgccatcac gctgacaccg gcaaccagcc caaccacacc gccgagcaaa ccaatagcgc
     8641 ctgcagagac gttgccgccc tctttagcgt taacatccgg tttctgtgaa cgcggagtct
     8701 gattttcgac gttggcaagc tgatggatgc ctttatggaa gccgatacct tcttcgttac
     8761 agccatagca tgggtgacca atcgctaccg gccacacgcc gccaacatcg cagaattgca
     8821 gcgttgagca gttgccgtaa gtttctggcc ctttacagcc gaggtggtac aggcaccagc
     8881 cttcacggtg gccttcatca ccgaactctt tggcaaaacg accagcatcg aagtgcgggc
     8941 gacgttcgca gtgttcgtga atcagacggc cataggcgaa ggtcggacga tttttgtcat
     9001 ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac ggtcgcgagg aagttgtgcg
     9061 ggttcggcgg gcagcccgga atattgataa cggttttgcc tggcagaact tcttgcaggc
     9121 tgactgcgcc agttgggtta actccagctg cggcaacacc gccccacgca gagcaggaac
     9181 cgatagcgat aatcgctgct gcgccttctg ccgctttgcg gatgtgatcc acaatcggct
     9241 caccagcaac catgcaataa ataccgttat cttttaatgg gatggaacca tccacaacca
     9301 acacatactg ccctttgtac ttctcaagtg cgttatgttt gttctcttcg acctgatgac
     9361 cgaaggcggc ggaaagcact tcgtgatact ccagagagat agtctccagc acgaggtttt
     9421 ctaccgttgg atgcgttgca cgaagcagag attccgtaca accggtgcac tcctgcgcgc
     9481 caatccagat aactggcgga cgctgcgggt tagtaaccga ttcggccatc tctgcagcgg
     9541 ctttgctact taaccccatg gtggcggcta atgctgcaca aagcttcatg aaatcacgac
     9601 ggttaatgcc gtgagaatgg atgagggtgt tatctccagt catttatagt tattccgttg
     9661 cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa taatgcgatc gatgcgccat
     9721 ttaccacaca tttattatgg ttatcgatat catgatactg cggcggggcg ctaaaacgaa
     9781 gggaagaagc attagtgtaa ttaattaaag caagataata cgtatgtttg atcaattttc
     9841 gaggcgataa ccggccataa aaaagcgcaa cgtagaacag gaattattct cacgaaagaa
     9901 atccattacc atctctttat ccagtccata gcggcgcgtc agaatacccg cttcccaggc
     9961 gctaagctgg cgatctccgg tcttttcgta catccggtgt gagtagccta atacaaaacc
    10021 gcgtttataa tcggcacaga attttgtcac attaaccgca ctgtcggcat gcgttgcttt
    10081 taatcccgcc attaagcctt taccaaaatg gttattcatc gttcacctca ttcgctatat
    10141 atcgccatat atagcgatat tttaggcaaa gaacgacttt tgcgcacgct attttttcat
    10201 gcttgtttaa gacgtaatcc agtaagagat aaacctatca ggagagatat ttatcgaacc
    10261 atttcagtgt cctttgccag gcaagatcgg cggcagattt gtcataacgg ggcgtggaat
    10321 cattatggaa tccgtgatta acccccggat agatatacgc ctcataaacc ttattattgg
    10381 ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat tcgggtgtcc agttccgcga
    10441 agtggagtag taaaggcgct tcaatcttcg ccacatcggc agtgggtgcc tgacgaccat
    10501 aaaacggcac cgcgcaggcc agttccggat acgcgacagc cgccgcgttc gatacgccac
    10561 cgccatagca aaatccggta atacccactt tgcctgccgc ttgcggatag cgttgcataa
    10621 actcaattgc ggcaaagaaa tcattcatca gtttggttgg atcaacctgc tgttgcagct
    10681 cacgaccttt atcatcattt cccggataac ctccaacgga acttaagccg tcaggtgcca
    10741 gggcgatata ccccgccttc gccactcgcc gtgccacatc ttcgatatac ggattcagtc
    10801 cacgattctc atgcaccact accacggctg gcgttttgcc gctcattttt gcgggtttca
    10861 ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg atacgtgatg tactcagcaa
    10921 caatctccgg gtcggtaaac tctacctgag tcgccagcgc ataatttggc ttgagcaaat
    10981 caaacaacgc taatgccgtc atcccgccca ccgcatactt cgccgcaagg ttgaggaact
    11041 cacgtttcga gattttcccg tgagcgtaat agtcgtagta atccaacaac tcttgtggga
    11101 aatctttggc ggttaaacgc ggcatcgttg cactcctcaa ctggtgtttt tttaagcaaa
    11161 gcataagcac gtgtttttgc ccagtttttc gtcactctgt gagccagact acgggatacg
    11221 cgctggcgaa tcgctaaact agaaacattg tttcgaaatt gaacggtgga aaggagaggt
    11281 catggtctgg ttagcgaatc ccgaacgtta cgggcagatg caataccgct attgcggaaa
    11341 aagcggttta cgcctgcccg cgttatcgct cggtttatgg cacaatttcg gtcacgttaa
    11401 cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt gatttgggca ttacgcactt
    11461 tgatttagcc aacaattacg ggccgcctcc aggaagcgca gaagagaact ttggtcgcct
    11521 gctgcgggag gattttgccg cttatcgcga tgaactgatt atctctacca aggctggcta
    11581 cgatatgtgg cccggccctt acggctctgg cggttcacgt aaatacctgc tcgccagcct
    11641 cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat atcttttact ctcatcgcgt
    11701 cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg
    11761 taaggcgctg tatgtcggga tctcctctta ctcgccagag cggacgcaaa aaatggtcga
    11821 gttgctgcac gagtggaaaa ttccgctttt aattcatcaa ccttcgtaca atttactgaa
    11881 ccgctgggtg gataaaagcg gcctgctgga taccctgcaa aataacggcg tgggctgtat
    11941 tgcctttact cctctggctc agggattgct gaccggaaaa tatctcaacg gcattccgca
    12001 agattcacgg atgtatcgtg aagggaataa agttcgtggt ctgacaccga aaatgcttac
    12061 cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg gcacagcagc gtggacaatc
    12121 aatggcgcaa atggcgttaa gctggttgtt gaaagatgat cgcgtgacgt cggtattgat
    12181 tggtgccagc cgcgcggagc aactagagga gaacgtgcag gcgctgaata atctgacatt
    12241 tagcaccgag gagctggcgc agattgatca gcatatcgcc gatggcgagc tgaatctgtg
    12301 gcaggcgtcg tccgataaat gacctgttaa taacgggcgc gcgaaccgcg cccgaatcag
    12361 atcagtgatt atgacgagtc agtcggtcaa gatagcccat cacaaatgca gaaagcacaa
    12421 acgtcagatg gataatgacg taccacatca gtttgttatc cgggacattt ttcgcatcca
    12481 taaagacgcg cagtaggtga atggaagaaa ttgccacaat cgacgctgct actttgtttt
    12541 tcagcgacgt tgcgtccatt ttccccagcc agttcagctt ctctttgttc tcggagatat
    12601 ccagctgtga gacgaaattc tcataaccgg aaaacatcac catcaccagc aaaccgccaa
    12661 ccagcgtcat atccaccagc gacagcaaca cgaggatcaa atctgattcc gccatcgaga
    12721 agatattcgg cagtacgtta ataatctcct ggaagaactt cagcgccagg gcaactaacg
    12781 ccagcgaaag gccaaagtac acgggggcaa gcagccagcg agaagcatac attgcatttt
    12841 caagaaaacg ttccatacat tcctgtcgtg tagttaaaac gggcaacagt atatcgcaat
    12901 tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta aacctttgtc gcataattct
    12961 gaggtttagg gatcttcctg gttcccttat caacgcatcg agagcgtcta cccttaaagg
    13021 acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa agacccgacc acgcagtatt
    13081 acactggtga atatcccaaa cagaaacaac cgacgccagg catccaggcg aagatgacac
    13141 cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag
    13201 cactggtgac agggggcgat tccggaatag gtcgcgctgc cgccatcgct tacgcgcgtg
    13261 aaggggctga cgtggcgatc agttatcttc ccgtggaaga agaagacgct caggatgtga
    13321 aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg
    13381 agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc gttaggcggg ctggatatta
    13441 tggcgctggt cgccgggaaa caggttgcca ttccggatat tgcagacctc accagcgaac
    13501 agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt ctggctaacc caggaagcga
    13561 tccccctgct accgaaaggt gcaagtatca tcaccacttc gtcaatccag gcataccagc
    13621 caagtccgca tttactggac tatgcggcta cgaaggcggc gattctgaac tacagccgtg
    13681 gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccta
    13741 tctggacggc actgcaaatt tccggcggac aaacgcagga taagatcccg cagtttggtc
    13801 agaaaacgcc gatgaagcgt gcggggcaac cggcggaact ggcccctgta tatgtttatc
    13861 tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca cggtgtgtgc ggcggcgagc
    13921 atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg gcatcattgt gaattcactc
    13981 ttacctgatg tggcgaaaac gccttatccg cctacaaacg catgaagatt caacatgttg
    14041 caatagattt tgtaggcctg ataagcgtag cgcatcaggc attgagcacc taatgccgga
    14101 tgcggcgtga acgccttatc cggcctacaa acgcacgaag attcaacatg ttgcaataga
    14161 tattgtaggc ctgatgagcg tagcgcatca ggcaattcta ctttacttcg ctttggcggt
    14221 ttcttcgccg accagaccta tcttcaggta acccgcctga tgcagcgtat ccattacctt
    14281 catcaacgtc tcgtaatcga cggttttatc cgctcggaag aagatggtgg tgtctttctt
    14341 gccttcggtt aacgcattca acgccgtaat cattgtttca tcggtgaccg gatcgttacc
    14401 gataaacatc gagttgtctg ccttcaccga cagataaacc ggtttttccg ggcgcggctg
    14461 cggcgtgctg gtagaagcag gcaagttcac cttcacatct accgtcgcta acggtgccgc
    14521 caccataaag ataatcagca gaaccaacat cacgtcgata aacggcgtca cgttgatatc
    14581 atgcatttcg ccgttatcgt cgaggttttc gttaagatgc attgccatcg gatattatcc
    14641 tgcgcgtaat ttttgtgcga cacgaaccgg atgcgcagcg gcgctggctt ccagatccag
    14701 gtcacggctt tgcagcaaca atacctgcgc tgcaacatca cccagcatcg ctttaaagcc
    14761 gccaatctgg cgtgcaaata cgttatagat aacgaccgca ggaatcgctg ccacgaggcc
    14821 gattgccgtt gctaacagag cttctgcgat acccggcgca acgactgcca ggttagtggt
    14881 ctgcgtttgc gcgataccaa taaagctgtt catgatgccc cagaccgtac caaacagacc
    14941 aacgaacgga gaaatcgcgc cgatggttgc cagatagccg ttaccgcggc ccatttgacg
    15001 acccactgcg gcgacccgac gctccagacg gaagctggta cgttctttaa taccttcgtt
    15061 atcgtcgctg ccttctgaca gttccagctc attctgcgct tcattgagca aatgcaggct
    15121 taagcttttg ctaccaaaat cagcggcgat atcgttggcc tggtttaagg aacgcgcttc
    15181 agccagcagt tgctgctcgc gcttaaggcg acgcttctga ttgaagaact ctacgctctt
    15241 actgaagaag attgcccaag tgactacgga ggccaaaata agcccaatca tcacgcactt
    15301 aacgacaata tcggcgtgct gatacatacc ccagacggaa aggtccgtct gcattaaatt
    15361 attacccact gtgtatctcc aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa
    15421 tgacgtcgaa ttgatagtcg ttctcattac tatttgcata ctgccgtacc tttgctttct
    15481 tttccttgcg tttacgcagt aaaaaagtca ccagcacgcc atttgcgaaa attttctgct
    15541 ttatgccaat tcttcaggat gcgcccgcga atattcatgc tagtttagac atccagacgt
    15601 ataaaaacag gaatcccgac atggcggaca aaaagcttga tactcaactg gtgaatgcag
    15661 gacgcagcaa aaaatacact ctcggcgcgg taaatagcgt gattcagcgc gcttcttcgc
    15721 tggtctttga cagtgtggaa gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt
    15781 tgttctatgg acggcgcgga acgttaaccc atttctcctt acaacaagcg atgtgtgaac
    15841 tggaaggtgg cgcaggctgc gcgctatttc cctgcggggc ggcagcggtt gctaattcca
    15901 ttcttgcttt tgtcgaacag ggcgatcatg tgttgatgac caacaccgcc tatgaaccga
    15961 gtcaggattt ctgtagcaaa atcctcagca aactgggcgt aacgacatcg tggtttgatc
    16021 cgctgattgg tgccgatatc gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg
    16081 aatcgccagg ctccatcacc atggaagtcc acgacgttcc ggcgattgtt gccgccgtac
    16141 gcagtgtggt gccggatgcc atcattatga tcgacaacac ctgggcagcc ggtgtgctgt
    16201 ttaaggcgct ggattttggc atcgatgttt ctattcaagc cgccaccaaa tatctggttg
    16261 ggcattcaga tgcgatgatt ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac
    16321 gggaaaacgc ctatctgatg ggccagatgg tcgatgccga taccgcctat ataaccagcc
    16381 gtggcctgcg cacattaggt gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg
    16441 ctgaatggct ggcagaacat ccgcaagttg cgcgagttaa ccaccctgct ctgcctggca
    16501 gtaaaggcca cgaattctgg aaacgagact ttacaggcag cagcgggcta ttttcctttg
    16561 tgcttaagaa aaaactcaat aatgaagagc tggcgaacta tctggataac ttcagtttat
    16621 tcagcatggc ctactcgtgg ggcgggtatg aatcgttgat cctggcgaat caaccagaac
    16681 atatcgccgc cattcgcccc caaggcgaga tcgattttag cgggaccttg attcgcctgc
    16741 atattggtct ggaagatgtc gacgatctga ttgccgatct ggacgccggt tttgcgcgaa
    16801 ttgtataaca ttgccacttt tggacaattt tgcagacatt taattgtgaa aagtcttaaa
    16861 ttgttgcgtc cgggatcaag gcgtcccgga cgaatcagga gtacaatagg cagataaagg
    16921 cttaaacgct gttccacagg aaagtccatg gctgttattc aagatatcat cgctgcgctc
    16981 tggcaacacg actttgccgc gctggcggat cctcatattg ttagcgttgt ttactttgtc
    17041 atgtttgcca cgctgttttt agaaaacggc ctgctgcccg cctcattttt gccaggcgac
    17101 agcttgttga tactggcagg cgcattgatt gcccaagggg ttatggattt tctgcctacg
    17161 attgcgattc tgaccgccgc agcaagtctg ggctgctggc taagttatat tcaggggcgc
    17221 tggttaggga ataccaaaac ggtgaaaggc tggctggcac agcttcctgc taaatatcac
    17281 cagcgcgcca cctgcatgtt tgaccgccac ggtctgctgg cgctgctggc tggacgtttt
    17341 cttgcatttg tccgtacgct gctgccaacc atggcgggaa tttccggtct gccaaaccgc
    17401 cgcttccagt ttttcaactg gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt
    17461 ggctatgcct taagtatgat tccgttcgtt aaacgccatg aagatcaggt aatgacgttc
    17521 ctgatgatcc tgccaattgc cttgttaacc gctggcttgt taggcacgct gtttgtggtg
    17581 attaaaaaaa aatactgtaa cgcctgacga ttttccccgt tcccggctgc tgtaccggga
    17641 acgtatttaa ttcccctgca tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc
    17701 gaagtaacgt ttaaactcac ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc
    17761 tgctgcgctg gccttcatgc cgtcatggat gatcatcatc cgcgccttat gcagacggta
    17821 attcttcaaa tactgcaacg gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc
    17881 cgatacgctc atgttggctt ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt
    17941 attctcaatc cgtttcagca cgcggctaat cagactgaag tgagtctggc gactgaccag
    18001 cgccagtaac gcgccgccgc aaggtccggt cagcacgtag tacagaattt cgcggatgat
    18061 ctgtttgccg agaatacgcg catccagtgg tcgctccatc acgtcgagta accgctccgc
    18121 cgcgcataaa atctcttctg ataacgtggc ggagttaatc ccgctggctg ccatcgacgg
    18181 ctggaaatgc tcatcttcgc caatgtccat caacagttcc tgtaactgca aaatatcgac
    18241 attgagacgc aaccctgcca gcggcacctc tgacgtggca taggtttcgc actcaaacgg
    18301 caacggcacc gtcagcagca ggtattcatt ggcatcataa cgaaacacgc gttcattgat
    18361 ataaccgatt ttatgcccgg aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt
    18421 acgtgcgaaa ggcgtctcgc catacaacaa acgtacatcg ggcaacagtt ctgacaaact
    18481 attttcttta attttcagtt tattaacttt atccgccagc aagcggcaaa tctcttcacg
    18541 tttcatatcg cgtaatttct taggaataat gcggcaattt gattgtgcgc aattttgtag
    18601 catttctcca gcactctgga ggaataggca agacattggc agaaatgagc attgagagcc
    18661 agggcgctgg cgatcacaat gaaaaacatc aggcagatcg ttctctgccc tcatattggc
    18721 ccagcaaagg gagcaagtaa tgaacaactt taatctgcac accccaaccc gcattctgtt
    18781 tggtaaaggc gcaatcgctg gtttacgcga acaaattcct cacgatgctc gcgtattgat
    18841 tacctacggt ggcggcagcg tgaaaaaaac cggcgttctc gatcaagttc tggatgccct
    18901 gaaaggcatg gacgtgctgg aatttggcgg tattgagcca aacccggctt atgaaacgct
    18961 gatgaacgcc gtgaaactgg ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg
    19021 cggttctgta ctggacggca ccaaatttat cgccgcagcg gctaactatc cggaaaatat
    19081 cgatccgtgg cacattctgc aaacgggcgg taaagagatt aaaagcgcca tcccgatggg
    19141 ctgtgtgctg acgctgccag caaccggttc agaatccaac gcaggcgcgg tgatctcccg
    19201 taaaaccaca ggcgacaagc aggcgttcca ttctgcccat gttcagccgg tatttgccgt
    19261 gctcgatccg gtttatacct acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga
    19321 cgcctttgta cacaccgtgg aacagtatgt taccaaaccg gttgatgcca aaattcagga
    19381 ccgtttcgca gaaggcattt tgctgacgct gatcgaagat ggtccgaaag ccctgaaaga
    19441 gccagaaaac tacgatgtgc gcgccaacgt catgtgggcg gcgacgcagg cgctgaacgg
    19501 tttgattggc gctggcgtac cgcaggactg ggcaacgcat atgctgggcc acgaactgac
    19561 tgcgatgcac ggtctggatc acgcgcaaac actggctatc gtcctgcctg cactgtggaa
    19621 tgaaaaacgc gagaccaagc gcgctaagct gctgcaatat gctgaacgcg tctggaacat
    19681 cactgaaggt tccgatgatg agcgtattga cgccgcgatt gccgcaaccc gcaatttctt
    19741 tgagcaatta ggcgtgccga cccacctctc cgactacggt ctggacggca gctccatccc
    19801 ggctttgctg aaaaaactgg aagagcacgg catgacccaa ctgggcgaaa atcatgacat
    19861 tacgttggat gtcagccgcc gtatatacga agccgcccgc taagcttttt acgcctcaaa
    19921 ctttcgtttt cgggcatttc gtccagactt aagttcacaa cacctcaccg gagcctgctc
    19981 cggtgagttc atataaagga ggaacgtatg gctaatccaa ccgttattaa gctacaggat
    20041 ggcaatgtca tgccccagct gggactgggc gtctggcaag caagtaatga ggaagtaatc
    20101 accgccattc aaaaagcgtt agaagtgggt tatcgctcga ttgataccgc cgcggcctac
    20161 aagaacgaag aaggtgtcgg caaagccctg aaaaatgcct cagtcaacag agaagaactg
    20221 ttcatcacca ctaagctgtg gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac
    20281 agcctgaaaa aactccagct tgattatatc gacctctact taatgcactg gcccgttccc
    20341 gctatcgacc attatgtcga agcatggaaa ggcatgatcg aattgcaaaa agagggatta
    20401 atcaaaagca tcggcgtgtg caacttccag atccatcacc tgcaacgcct gattgatgaa
    20461 actggcgtga cgcctgtgat aaaccagatc gaacttcatc cgctgatgca acaacgccag
    20521 ctacacgcct ggaacgcgac acacaaaatc cagaccgaat cctggagccc attagcgcaa
    20581 ggagggaaag gcgttttcga tcagaaagtc attcgcgatc tggcagataa atacggcaaa
    20641 accccggcgc agattgttat ccgctggcat ctggatagcg gcctggtggt gatcccgaaa
    20701 tcggtcacac cttcacgtat tgccgaaaac tttgatgtct gggatttccg tctcgacaaa
    20761 gacgaactcg gcgaaattgc aaaactcgat cagggcaagc gtctcggtcc cgatcctgac
    20821 cagttcggcg gctaacatgc aaattctccc ggtggcggta atgttccgct accggacttt
    20881 tcagaaatca tttattcccc tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg
    20941 ccaaatataa ggacatcatc atgcagagcc ggaagctctt aaaagaacaa ctcatctata
    21001 tccgggataa acgcaacgga gaggtgaaaa acagatgaaa ataatacttc tgtttttagc
    21061 agccctggca agttttaccg tacacgcaca gcccccctca ctgaccgtag aacaaacagt
    21121 ccggcatatt tatcagaact ataaatcaga tgccactgcc ccttattttg gtgaaaccgg
    21181 agagcgggcg ataacttctg cgcgtattca acaggcgctt accctgaacg acaatcttac
    21241 gctgccgggc aatattggct ggctggatta tgatccggtt tgtgattgtc aggattttgg
    21301 cgatctggtg ctagaaagcg ttgcgataac ccaaactgac gccgatcatg ccgatgccgt
    21361 tgtgcgcttt cgtatcttta aagatgataa agaaaagacc acgcagacac tgaaaatggt
    21421 ggcggaaaat ggtcgttggg tcattgacga tattgtcagc aatcatggca gcgtcttaca
    21481 agcagttaat agcgagaatg aaaaaacgct ggccgcttta gcttcgttgc aaaaagaaca
    21541 gccggaagcc tttgttgccg aactctttga acatattgct gattatagct ggccgtggac
    21601 gtgggtggtt tccgactctt accgccaggc ggttaatgcc ttctataaaa ccaccttcaa
    21661 gacggccaat aatcccgatg aagatatgca aatagaacgg caatttattt acgacaatcc
    21721 gatctgtttt ggcgaagagt cgctattttc acgcgttgat gaaattcgag tcctggagaa
    21781 aaccgccgat tccgcccgta ttcatgttcg ttttacgctg accaatggca acaacgaaga
    21841 gcaagaactg gttttacagc ggcgcgaagg caagtgggaa atcgctgatt ttatccgccc
    21901 gaacagcggc agcctactta agcagattga ggcaaaaacc gccgccagat taaagcaatg
    21961 agctgaatta aataacaatt agccggaaca ataaataaaa gggaacacta tatgaaaacg
    22021 attttcaccg tgggagctgt tgttctggca acctgcttgc tcagtggctg cgtcaatgag
    22081 caaaaggtca atcagctggc gagcaatgtg caaacattaa atgccaaaat cgcccggctt
    22141 gagcaggata tgaaagcact acgcccacaa atctatgctg ccaaatccga agctaacaga
    22201 gccaatacgc gtcttgatgc tcaggactat tttgattgcc tgcgctgctt gcgtatgtac
    22261 gcagaatgat aaaaaaatcc ccggcagcat gtcagttgcc ggggattttt tttaacgtcc
    22321 aaccgccgct ttagggcgtt tcttcgcacc agcattcacc ggacgagatt gcgtagacga
    22381 cgcttttttt gccgtagcag gcgtctgacg ctgggtcgcc atcggcgtat gtttcgtcaa
    22441 cgccggacgg gtattgcggt tctggcgacg agcttcacgc atctcttcaa tggttggcgc
    22501 aggcactaag caatcgcgac ggctgccaat cagatgcttt ttgcccatcg cttccagcgc
    22561 ctggcggatt aacggccagt ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg
    22621 acgctgtttg tcgcccttcg gtacgaagac gtcttcgctc ttataaccaa tcttcgccag
    22681 cgggtttttc ccggtgtaat acatggtggt tgagttcgcc agcggcgatg ggtagaagtt
    22741 ctgtacctgg tcgagacgga aacgatgctt tttcagccac agcgccagat tcaccatatc
    22801 ttcatcacgc gtaccggggt gcgcggagat gaaatagggg atcagatact gctctttacc
    22861 tgcctgtttt gagtaagtat cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg
    22921 cttcatcatc ttcgataacg gcccttcttc ggtatgttcc ggggcaatct tcagataacc
    22981 gccgacgtga tgggtcgcca actctttgat atagcgcgga tcttccacgg cgatgtcata
    23041 acgtacgcct gaggcgatga ggattttttt aatgcctttc agatcacgcg cacggcgata
    23101 gaggttgatc gtcggttcgt ggttagtgtc catgtgcgga caaatatccg gataaacgca
    23161 cgacaaacgg cgacaagttt gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt
    23221 ggcagttggc ccaccgagat cggaaattac gcccgtaaaa ccaggaacgg tgtcgcggat
    23281 cgcttcgatc tcattaatga tcgaatcttc cgaacggctc tgaataatgc gcccttcgtg
    23341 ctcggtgata gaacagaaag agcagccgcc aaagcagccg cgcataatgt tgaccgagaa
    23401 acggatcatt tcgtaagccg gaatacgggc attgccatag gccggatgcg gcacgcgctt
    23461 gtacggcagc gcaaaaacgc tgtccatctc ttcggtagaa agcgggatag caggcgggtt
    23521 gatccacaca tagcggtcac cgtgtttttg catcaatgcg cgggcgcagc ctgggttggt
    23581 ttcgtggtgc agaatgcgcg aagcatgagc gtacaggact ttatcgccct tcactttctc
    23641 gaaagaaggc agcaatacgt aggttttttc ccacggtttc gggcgcggcg gctgcacggt
    23701 tacagctttc gcttcctgct ttttcggcgc aacaggtttg ttatccgcgc acggcagatc
    23761 ttcgccatac ggatgcggga ttgggtcgat tttccctggg gtatcaagac gagtagaatc
    23821 cacaccgctc cagccaggca gcgcctcttt tacgataatt gcggtattac gcacatcgcg
    23881 gatttcgcta attggctcgc ccatcgccag acgatgcgct acttccacca gcggacgttc
    23941 accgttacca aacatcagca tgtcggcttt cgaatccacc agcacggaac ggcgcacggt
    24001 atcggaccag taatcataat gcgcggtacg gcgcagacta gcctcaatac cgccaaggat
    24061 caccggcaca tctttccacg cctctttaca acgctgggta taaaccagtg tggcgcgatc
    24121 cggtcgctta cccgcgacgt tatccggtgt gtaggcatcg tcatgacgta aacggcgatc
    24181 ggcggtatag cggttgatca tcgaatccat gttgccagca gtaacaccaa aaaacagatt
    24241 cggtttaccc agacgcataa agtcgtcttt gctgctccag tccggctggg cgatgatccc
    24301 gacgcgaaag ccctgcgctt ccagcatacg accgcaaatc gccatcccga agcttgggtg
    24361 atcgacatac gcgtcgccag taaccaaaat gatgtcgcag ctatcccagc caagttgatc
    24421 catctcttca cgagacatcg gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg
    24481 cggccaggag aacaggtcgc gatccggttg gatcagggag atagagctca ttttgcttcc
    24541 agaaatgata aaaaaataat caaaggccgg ggattataag ccggaacgaa agagaaatcg
    24601 aaaggtattc catactcgcc ctcctcgggc gagtatgaag attacggtac cggattgacc
    24661 aacagttgcc caatcgagcc acggtccgcc atttccagcg tctgactgtt gaagtagaac
    24721 gggaagtgcg cccaggaagg ctgaccgaaa taaacaagca gctccacttg tccgtcaacc
    24781 caaacggtat ctttccagcc tctgtcttcc gggaacggca tcgcgccatt cacgttacgg
    24841 atctggaaca tcacgccttc aatatggaac gcttgcggct cgtccgcgcg taccgtccag
    24901 cgttcccacg ttccttgctg cgcggtgaca tcaatacggt tgacgtccca cagctgtcca
    24961 ttaatacccg ggtcatcacc cagactaata tcgcgactgc gaatcggcga accggccatg
    25021 atttcagttg gcagcaagcg catcggaaga ctgtctgtga ccagcggcag aaggccggtt
    25081 gggcgcagcg ttagcaccag ggtagaaacc aaaatgctgg atggctcaaa gaagccacga
    25141 atacgatcaa caatgctcgc cgcttcgcca caggtgatcg acacttcatc accgttgctc
    25201 atatccacca gaatctcgcg gcgctcgccc ggtgccagcg aaagttgctt caccgataca
    25261 ggagcaggca ggaatccctg atcgccagaa atcacatgta acgggcgacc atcgctcatc
    25321 tgtagttgat agcgacgaga gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc
    25381 gagacttcaa cgtacgggct ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg
    25441 ccgcttcccg gttcgttgta ttctggcgta ccaaagttat ccagccgttt atcctggata
    25501 atgaccggaa aatcatccac accataatgg ttggggatag gcagcgactt gctgacttca
    25561 tcttccacca gccacattcc ggcaaggccg ttatagacct gctgagccgt gcggttggga
    25621 gtattggcgt gataccacag agtagctgcg ttctggcgaa tgggcagtac gggtgcccag
    25681 tcagcgtttg gcgacatcat ccgtgccgga ccgcccatca gcggacctgg tacctgtagc
    25741 ccggccaccg tcattgagac attttctgtc aggcggttgc tgtaaataag cttaacatcg
    25801 tcgcccttcc agacgcggat agtcggcccc aggtaacgac cattgattcc ccagaccgac
    25861 gcgcgtgtcc ctggcgtaaa tgaccagtgc gcacgttgta cagtcataaa cagcggttgc
    25921 ccacggcgag attcaagtag cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg
    25981 gccttcaggg gaacagcgcc tgcacaaagt gcaatccccg atgcctgaat gaactgacgc
    26041 cgactgagtg acatatttgc tccatgtaaa actggctaaa gtaataccgt gcaggaaagt
    26101 gttccccgca aaaatacaga ttgccgactt aaacttttcc ggcggcttcg cgttctgcga
    26161 cttctttatc gagctcggcg attttttgtt ccattatcga acgacaatgg gcagccagct
    26221 cacgaacctg atctttgcca tactgactga cgtcaattgg cggcagcatt tcgacaatca
    26281 ccagaccgtt gtgcagtcga ttaagattaa tcttattcga agttgtagag acgcacacgg
    26341 gaataatcgg gacgcccgcc gcaattgccg cgtgaaatgc tccagtcttg aacggtagca
    26401 ggccgcgacc acggctgcgg gttccttccg ggaacatcca gatggaaata cggcgttttt
    26461 tgaaatgatt cacgacttcc gcaatggtgc cgtgagcttt agtgcgattg tttctgtcga
    26521 tcaataagtt gccggttaac cagtacaact gcccgaagaa ggggatccac agcaagctct
    26581 ttttacctac cgtcaccgtc ggcggttgca caatgttcga tgctgtcacc atgtcatagt
    26641 tgttctggtg gttagcgata tagatagcat tgccgtagct ttcagcatct gcaggtttgc
    26701 ggcactcaac tttcaggccg aacagcggcg caagacggcc aaacatatgc ccaaaggtgg
    26761 ccacatgttt cgggttacgc gggctgaaaa ggcagtaaat ggagccgaat acacagacta
    26821 agatgctgta aatcacggta ataataagac gaaagatata tagcataaca ccctctgaag
    26881 gtctgagaga ctggcattgt acgtcacctg aaatcaggtt agggcattgt taaaagccgc
    26941 tctctggaaa agcgtattgt taatcgcaac gcatgaataa acaacggttt caagggaatt
    27001 tttatcgttt ccgggggttg aatacgctgc cggatgacga cttaacgttt catccggcgt
    27061 tccttgcaag cgggaggaaa caggccctcc ccggcatatt actcttcgct atcaccgctg
    27121 ctggcacggc gaggagagtc gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac
    27181 gtaccgcggc gtccacgttc gccagtgact ttctgtaact cttccgggcg cagtttaatt
    27241 ttgcgtttcc caacatgaat ggtcagcgtg ctttgcggcg gcagaacgta caattgcgcc
    27301 agaccatctt ctccacgcgc ggcttctgcc gatggaatgt tgataatctt gttgcctttg
    27361 cccttcgaca gctgcggcag atcgcttacc gggaacatca acatacggcc tgcctgagtg
    27421 attgccagca gcatatcgga agcatcttca atcaccaccg gcggcataac atgggcattt
    27481 tccggtaagg tgatcaaagc cttacctgca cggttacgcg ccaccagatc gttaaaggtg
    27541 cagacgaaac cgtaacccgc atcggaagcc atcagcagtt tctgatcgtc gctttccatc
    27601 agcatatggt caacggtcgc cccaggcggc aacgttaatt tgccggtgag tggttcgccc
    27661 tgaccacgcg ccgacggcag cgtaatcggg tcgatggcat agctacgacc ggtggaatca
    27721 acaaacacta ccggttggtt gctcttacct ttcaccgccg ctttgaagct atcacccgct
    27781 ttataattca ggcccggcgc gtcgatatca tggcctttag cgctgcgtac ccagcccatc
    27841 tgcgacagca caatggtgac aggttcagac ggcagcatgt cgtgctcgct catcgctttc
    27901 gcttcttcgc gttcctgcaa cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc
    27961 tgcagttctt tcttcagcag gttattcatt ttacgctcgg aagccaaaat gccctgcaac
    28021 tggtcgcgct ctttttccag ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg
    28081 gcaagatgac gcagtttcag ttcgaggatc gcttccgcct gggtttccgt aaggccaaac
    28141 cgcgacatca gcgccggttt cggttcatct tcgttacgaa tgatctcaat cacttcgtcg
    28201 atgttgagaa acgccaccag caaaccttcc aggatatgca ggcgcttgag gactttctcc
    28261 agacgatagt tcagtcggcg gcgcacggta tcgcgacgga acaccagcca ttcggagagg
    28321 atttccagca ggtttttcac cgccggacga ccatccagac cgatcatatt gaggttaatg
    28381 cgatagctct tttccagatc ggtggtagcg aagaggtggt tcatcacctg atccatatcc
    28441 acgcggttgg aacgcggcac aatcaccaga cgggtcgggt tctcgtggtc agattcatcg
    28501 cgcagatcgt caaccatcgg cagctttttg ttgcgcattt gcgcagcaat ttgctccagt
    28561 acgcgcgcac ctgaaacctg atgcggtaat gcgctgataa ccaccgcgcc atcttctttc
    28621 ttccacaccg cgcgcatacg cactgaacca cgtccgttct cgtagatttt acggatctcg
    28681 gcgcgcgaag tgataatttc cgcttcagtc ggataatccg gcccctgcac gatatccagc
    28741 agctgctcga gcgtggtttt cggctggtcg attaatgcga ttgccgcctg agctacttca
    28801 cgcaggttat gcggtggaat atcggtcgcc atgccgacgg caataccggt ggtgccgtta
    28861 agcaaaatgt ttggcagacg agcaggcagc attttcggct cctgcaaagt gccgtcgaag
    28921 tttggcaccc agtcagccgt cccctgcccc agctcgctca atagcagctc ggaatatttc
    28981 gacaaccggg attcggtgta acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc
    29041 cagttcccct gaccatcaac cagcggataa cggtaagaga acggctgcgc catcaggacc
    29101 atcgcttcat aacaggcgct atcgccgtgc ggatggtatt tacccagtac gtcaccgacg
    29161 gtacgggccg attttttaaa tttggcgctg gcattcaggc ccagttcaga catcgcatac
    29221 acaatgcggc gctgaacagg tttaagacca tcgccaataa acggcaacgc acggtccatg
    29281 atcacgtaca tggagtagtt taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc
    29341 tctgccatat cgctcattaa ttctgattcc tcaacttatt cgccagcctt caaaggggca
    29401 atattgccgg agatactaca ttatctgccg cgtcgagtca caggagatca gcgtgaatag
    29461 cgccgccgga cgtgctccga cggcgataaa ggcatcaatg tgccttgata tacaactctt
    29521 tgctgtagac ttgatcggca tagagggcag cacaaggttg cgcccatgcc acaccactcg
    29581 gcatgggggt ccgctccggg cgattcgaga agttgaggtt atgcgagtcc agggaagcct
    29641 aaagcatcaa agtagtcgac tgtaagtata cggttcagcc cagtttagct gtgtctccac
    29701 cagtgccccg cgcctcctgc cataagtgcg gcttgctcat ggataccccc gagacggctt
    29761 acggcgcacc ggataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa
    29821 gcccaacaac aatacgcctg tggcattgtt cgctcaaact tatcgcgagt gatttggctc
    29881 acactccggt aaagattccc catcacgcca acggcacata aacatccgtt tccagtcgct
    29941 gctctgtcac cccttcggca agattggtgt agtgaaacat aatcggtgct ttacgcattt
    30001 tctcgccgct tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt
    30061 ccagctcgcc aacgtggcgg gccacggcat aacgtccacc ggtaagttca ccattgctca
    30121 caccataacg attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg
    30181 cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa
    30241 caggagaaag ccccgtctct ttacgccaca tgataaattt cgccacactg tagttgagca
    30301 actcagggct acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt
    30361 ttacatccag tgtcaggttt gtcattttgc ctccctgcga aattattccc gggaacgcgt
    30421 cccgggagcg gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca
    30481 gaattccagt ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc
    30541 acaccgttcc agcgtttgtg gtcgatatca acattgatag taccgctggc atctttaaac
    30601 acgtagagat cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg
    30661 tcacgcaggg attttgcgct ttctacggtc gttacgctgc cgttcggccc ctggaatcct
    30721 ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc
    30781 ggtgcgctgc acagggccat tactgcgatt actgctgcga attttttcat gtttattact
    30841 ccctttaatg tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa
    30901 aaaaataaaa tttagtgctg tacagagcgc gttacaacac ggtttactgg cagcaaatac
    30961 ggttatcgca gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggc
    31021 gacggcatca aaacgggcct tagtaaaatg ggttttagcg tcgactggtt tacacaaggt
    31081 cgtcagggaa aagaggcgct ttatagcgcg ccttatgatg cggtgatcct ggatttaacc
    31141 ttaccaggca tggatggtcg cgatattttg cgcgaatggc gagaaaaagg tcagcgtgag
    31201 ccggtactga tcctgaccgc gcgcgatgcg ctggaggaac gtgtagaagg gctgcgtctg
    31261 ggagctgacg attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggaa
    31321 gctctgatgc gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgtcatg
    31381 ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa
    31441 gaatttgccc tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg
    31501 attgaagaga aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat
    31561 gtgcatcatc tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc
    31621 tacacattag gtgagaaatg aaatttaccc aacgtcttag tctgcgcgtc aggctgacgc
    31681 taatcttttt aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac
    31741 aaacaacgga taacgtcgat gaattgttcg acacccaact gatgctgttt gccaagcggt
    31801 taagtacgct cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata
    31861 aattaaaaca cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg
    31921 gcagaatggt ccttaacgat ggcgataacg gagaagatat tccttatagc tatcaacggg
    31981 aaggttttgc tgacgggcaa ctggtgggcg ataaagatca atggcgtttt gtctggatga
    32041 cctcacctga tggcaaatat cgcatcgttg ttggtcagga atgggaatac cgtgaagaca
    32101 tggcgctggc gattgttgcc gggcaattga tcccgtggct ggtcgcactg ccgattatgt
    32161 taatcatcat gatggtacta ttgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg
    32221 cgctgcgtat gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg
    32281 aagtgcgtcc gttggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg
    32341 ttcgtgaacg acgctttacc tccgacgcag ctcacgaact acgtagcccg ttaacggcgc
    32401 tgaaagtgca aaccgaagtt gcgcagcttt ctgacgatga tccgcaagcg cggaaaaaag
    32461 cgctgcttca attacattac gggatcgatc gcgctactcg tctggttgat caactgctca
    32521 ctctatcgcg gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag
    32581 atctcctgca atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg
    32641 tgcgactgac actcaatgcc cacggcatca aacgcaccgg acaaccgcta ttgctaagtt
    32701 tgttggtgcg aaatttgctg gataacgccg tgcgctacag tccacagggc agcgtgatag
    32761 acgtcacgct gaatgctgat aatttcatcg tgagggataa cggccccggt gtgacgccag
    32821 aggctctggc gcgaattggc gaacgcttct atcgcccccc cggacaaacc gctaccggca
    32881 gtggacttgg gctatcgatt gtccagcgaa tcgccaaatt gcatggcatg aatgttgagt
    32941 ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag ctggtaactt ctctgtctat
    33001 gagagccgtt aaaacgactc tcatagattt tactaatagc aaaatataaa ccgtccccaa
    33061 aaaagccacc aaccacaaac cagacaaata ccgagcgaga atacatatac ccaaaactcg
    33121 aacatttccc gcataaagag ttttcttaag ataagaataa taagtggcgt aagaagaaaa
    33181 aatgctgcaa tcattgcttg caatatacga tcttcgcctg aaacggaagg tatactgcaa
    33241 atattgtctc ccgactcaag catccagtca tacttattga aagcaaaaaa agcatctatt
    33301 aaccaataag taagcattat gtataacaga gcttcaaata ttgtttgaat tttatg
//
LOCUS       DAZFKM010000042        33197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFKM010000042 DAZFKM010000000
VERSION     DAZFKM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..860
                     /gene="yccA"
                     /locus_tag="ACHKAY_002091"
     CDS             201..860
                     /gene="yccA"
                     /locus_tag="ACHKAY_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO5700770.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            951..1280
                     /gene="tusE"
                     /locus_tag="ACHKAY_002092"
     CDS             951..1280
                     /gene="tusE"
                     /locus_tag="ACHKAY_002092"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO5700771.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(1277..1555)
                     /gene="yccX"
                     /locus_tag="ACHKAY_002093"
     CDS             complement(1277..1555)
                     /gene="yccX"
                     /locus_tag="ACHKAY_002093"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO5700772.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            1650..2840
                     /gene="rlmI"
                     /locus_tag="ACHKAY_002094"
     CDS             1650..2840
                     /gene="rlmI"
                     /locus_tag="ACHKAY_002094"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO5700773.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            2898..3215
                     /gene="hspQ"
                     /locus_tag="ACHKAY_002095"
     CDS             2898..3215
                     /gene="hspQ"
                     /locus_tag="ACHKAY_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO5700774.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(3260..3673)
                     /gene="yccU"
                     /locus_tag="ACHKAY_002096"
     CDS             complement(3260..3673)
                     /gene="yccU"
                     /locus_tag="ACHKAY_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO5700775.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            3846..4508
                     /gene="yccT"
                     /locus_tag="ACHKAY_002097"
     CDS             3846..4508
                     /gene="yccT"
                     /locus_tag="ACHKAY_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO5700776.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            4604..5062
                     /gene="mgsA"
                     /locus_tag="ACHKAY_002098"
     CDS             4604..5062
                     /gene="mgsA"
                     /locus_tag="ACHKAY_002098"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO5700777.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5094..7148)
                     /gene="helD"
                     /locus_tag="ACHKAY_002099"
     CDS             complement(5094..7148)
                     /gene="helD"
                     /locus_tag="ACHKAY_002099"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO5700778.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            7271..7717
                     /gene="yccF"
                     /locus_tag="ACHKAY_002100"
     CDS             7271..7717
                     /gene="yccF"
                     /locus_tag="ACHKAY_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO5700779.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            7727..9889
                     /gene="yccS"
                     /locus_tag="ACHKAY_002101"
     CDS             7727..9889
                     /gene="yccS"
                     /locus_tag="ACHKAY_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO5700780.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(9852..10481)
                     /gene="sxy"
                     /locus_tag="ACHKAY_002102"
     CDS             complement(9852..10481)
                     /gene="sxy"
                     /locus_tag="ACHKAY_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO5700781.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            10700..11209
                     /gene="sulA"
                     /locus_tag="ACHKAY_002103"
     CDS             10700..11209
                     /gene="sulA"
                     /locus_tag="ACHKAY_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO5700782.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            11566..12606
                     /gene="ompA"
                     /locus_tag="ACHKAY_002104"
     CDS             11566..12606
                     /gene="ompA"
                     /locus_tag="ACHKAY_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO5700783.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(12681..13133)
                     /gene="matP"
                     /locus_tag="ACHKAY_002105"
     CDS             complement(12681..13133)
                     /gene="matP"
                     /locus_tag="ACHKAY_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO5700784.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            13319..15079
                     /gene="ycbZ"
                     /locus_tag="ACHKAY_002106"
     CDS             13319..15079
                     /gene="ycbZ"
                     /locus_tag="ACHKAY_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5700785.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            15148..15666
                     /gene="fabA"
                     /locus_tag="ACHKAY_002107"
     CDS             15148..15666
                     /gene="fabA"
                     /locus_tag="ACHKAY_002107"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO5700786.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(15736..15903)
                     /gene="rmf"
                     /locus_tag="ACHKAY_002108"
     CDS             complement(15736..15903)
                     /gene="rmf"
                     /locus_tag="ACHKAY_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO5700787.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16159..16722)
                     /gene="pqiC"
                     /locus_tag="ACHKAY_002109"
     CDS             complement(16159..16722)
                     /gene="pqiC"
                     /locus_tag="ACHKAY_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO5700788.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            complement(16719..18359)
                     /gene="pqiB"
                     /locus_tag="ACHKAY_002110"
     CDS             complement(16719..18359)
                     /gene="pqiB"
                     /locus_tag="ACHKAY_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO5700789.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(18364..19617)
                     /gene="pqiA"
                     /locus_tag="ACHKAY_002111"
     CDS             complement(18364..19617)
                     /gene="pqiA"
                     /locus_tag="ACHKAY_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO5700790.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(19747..21654)
                     /gene="uup"
                     /locus_tag="ACHKAY_002112"
     CDS             complement(19747..21654)
                     /gene="uup"
                     /locus_tag="ACHKAY_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5700791.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(21666..23774)
                     /gene="rlmKL"
                     /locus_tag="ACHKAY_002113"
     CDS             complement(21666..23774)
                     /gene="rlmKL"
                     /locus_tag="ACHKAY_002113"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO5700792.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            24018..25127
                     /gene="ycbX"
                     /locus_tag="ACHKAY_002114"
     CDS             24018..25127
                     /gene="ycbX"
                     /locus_tag="ACHKAY_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO5700793.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(25124..25666)
                     /gene="zapC"
                     /locus_tag="ACHKAY_002115"
     CDS             complement(25124..25666)
                     /gene="zapC"
                     /locus_tag="ACHKAY_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO5700794.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(25840..26850)
                     /gene="pyrD"
                     /locus_tag="ACHKAY_002116"
     CDS             complement(25840..26850)
                     /gene="pyrD"
                     /locus_tag="ACHKAY_002116"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO5700795.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(26961..27698)
                     /gene="ycbF"
                     /locus_tag="ACHKAY_002117"
     CDS             complement(26961..27698)
                     /gene="ycbF"
                     /locus_tag="ACHKAY_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO5700796.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(27664..28179)
                     /gene="ycbV"
                     /locus_tag="ACHKAY_002118"
     CDS             complement(27664..28179)
                     /gene="ycbV"
                     /locus_tag="ACHKAY_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5700797.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(28187..28729)
                     /gene="ycbU"
                     /locus_tag="ACHKAY_002119"
     CDS             complement(28187..28729)
                     /gene="ycbU"
                     /locus_tag="ACHKAY_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5700798.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(28741..29811)
                     /gene="elfG"
                     /locus_tag="ACHKAY_002120"
     CDS             complement(28741..29811)
                     /gene="elfG"
                     /locus_tag="ACHKAY_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5700799.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(29802..32402)
                     /gene="elfC"
                     /locus_tag="ACHKAY_002121"
     CDS             complement(29802..32402)
                     /gene="elfC"
                     /locus_tag="ACHKAY_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5700800.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(32427..33128)
                     /gene="elfD"
                     /locus_tag="ACHKAY_002122"
     CDS             complement(32427..33128)
                     /gene="elfD"
                     /locus_tag="ACHKAY_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5700801.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         8008 a         8695 c         7941 g         8553 t
ORIGIN      
        1 ataaggggtt agctaaatgc taaccccttt ttcttttgcc tgtcgaaatt ctcagggcgt
       61 tatatttgct taatgacctg ataatccgct gttaaacctg ttccagatca aatgcgtaaa
      121 gatgggtaaa acttctgggt gcccttacgc attatcatta tgctgcttaa ttaattacat
      181 ctgtcataga gagtgactca atggatcgta ttgttagttc ttcacatgac cgtacatcac
      241 tgcttagcac ccataaggtg ctgcgtaata cctattttct gctgagcctg acgctggcct
      301 tttcggcaat taccgcaact gccagtacgg tgctgatgct gccatctccg ggtctgattc
      361 tgacgctggt gggtatgtat ggtttgatgt tcctgaccta taaaacggcg aataagccga
      421 ccgggattat ctccgcattc gcctttaccg gttttctggg ttatatcctc gggcctattc
      481 tgaacaccta tctgtctgcc ggaatgggtg acgtaatcgc tatggcactg ggcggaacgg
      541 cgttagtgtt cttctgctgc tctgcatatg tgctgaccac ccgcaaagat atgtcgttcc
      601 tcggcggtat gctgatggcg ggtattgtgg tggtgctgat tggtatggtt gctaatatct
      661 tcctgcagct gcctgctctg catctggcga tcagcgcggt cttcattttg atctcctctg
      721 gcgctatttt gtttgaaacc agcaacatcg ttcatggcgg tgaaacgaac tatattcgtg
      781 ccacggttag cctgtatgtt tcgctgtaca acatcttcgt cagcctgctg agcattctgg
      841 gcttcgccag ccgcgattaa tctcacccgc taacactttc atagcctcgc tttatgcggg
      901 gctttgtttt ttgttacact gccgccagtc tgattaaggt agtaataatt atgctgatct
      961 tcgaaggtaa agagatagaa acggataccg aaggctatct caaagaaagc agccagtgga
     1021 gtgagccgct ggcagtggtg attgcagaga acgaagggat ttcgctgtca ccggaacact
     1081 gggaagtggt gcgttttgtg cgtgatttct atctggaatt caatacttct ccggcgattc
     1141 gtatgctggt aaaagcgatg gcgaataaat ttggcgaaga aaaaggtaac agccgctatt
     1201 tataccgact gtttccgaaa ggtccggcta agcaagccac caaaattgct ggcctgccaa
     1261 aaccggtaaa atgtatttaa cggatacgaa aatcggttaa ttcccccgag ggatgatgcg
     1321 gctcgctgag cacccgttct acacgcgcag aacgcgggcc gccactcttt aaccacagca
     1381 ttaatttttc cacctgccct tcttcaccgc aggcaaccac ttccacgctg ccatcgtcaa
     1441 gatttttggc gtacccggtt aaacccagtc ttttcgcttc gtactgtgtg gtgtagcgaa
     1501 atcctacgcc ctgaactcgc ccgtaaaccc aggcaattat gcagactttc gacattgctg
     1561 ctctccttat ccttaatgga cgcgttgcaa atccatggaa aactccggac aatgacgccc
     1621 atttctttgc atcgacagaa aagtaaatta tgagtgtacg tttagtgtta gccaaagggc
     1681 gcgaaaaatc attacttcgt cgccatccgt gggtcttttc cggggccgtt gcccgcatgg
     1741 aaggtaaagc cagcctcggt gaaaccatcg atattgttga tcatcaggga aaatggttag
     1801 cacgcggcgc ttattcacca gcttcgcaaa tccgggcgcg cgtctggacg tttgacccgt
     1861 ctgagtctat cgacattgct tttttttccc gccgtttgca acaagcacaa aaatggcgtg
     1921 actggctggc gcaaaaagat ggcctcgaca gctatcgttt aatcgccgga gaatctgatg
     1981 gcctgccggg tattactatc gatcgtttcg gtaattttct ggtgctgcaa ctgctgagtg
     2041 ctggggcaga atatcagcgc gcggcattag ttagtgccct gcaaacgctg tacccggaat
     2101 gtgcgattta cgatcgcagc gatgttgcgg tacgtaaaaa agaagggatg gagctgaccc
     2161 agggcctcgt caccggcgag ttgccgcctg ccctgctgcc gattgaagaa cacggcatga
     2221 agctgctggt ggatattcag cacggacaca aaacgggcta ctacctggac cagcgtgata
     2281 gccgcctggc tacccgccgc tacgttgaaa ataaacgtgt gctgaactgt ttctcctata
     2341 ccggtggttt cgccgtatcg gcactgatgg gcggttgcag ccaggttgtc agcgttgata
     2401 cctcccagga agcgctggat attgcacggc agaacgttga gctgaacaaa ctggatctga
     2461 gcaaggctga gtttgtccgt gatgatgtct ttaaattgct gcgtacttat cgcgatcgcg
     2521 gtgaaatatt tgacgttatc gtgatggacc cgccgaagtt tgttgagaat aaaagccagt
     2581 tgatgggcgc gtgtcgtggc tataaagata tcaacatgct ggcgattcag ttgctgaatg
     2641 aaggcggtat tctcctgact ttctcctgtt ccggtctgat gaccagcgat ttatttcaga
     2701 aaatcatcgc ggatgccgca attgatgccg gccgtgatgt acaatttata gagcagttcc
     2761 gtcaggcagc cgatcatccg gtgatcgcta cctatccgga agggctatat ctgaaagggt
     2821 ttgcctgtcg cgtcatgtaa cttgaaatgt ggaattttac ccttatatca aaggtaaaga
     2881 tttcccggga ggtgactatg attgccagca aattcggtat cggccagcag gtccgccatt
     2941 ccctgttagg ttatctcgga gtggtcgtgg atatcgaccc ggtttattcg ctctctgaac
     3001 cgtcgcctga tgagctggcg gtgaatgacg agcttcgcgc cgctccgtgg tatcacgtgg
     3061 tgatggagga cgataacggc ctaccggttc atacctacct ggctgaagcc cagctaagta
     3121 gcgaattgca ggatgaacat cctgaacagc catccatgga tgagctggcg cagaccatcc
     3181 gcaaacaact ccaggccccg cgtctgcgta actgatccct gaacgaatcc ccggaaggtt
     3241 tttgccttcc ggggattttt tatttggcca ggcccaggcg agggatctca atagccgggc
     3301 agcgatccat caccacattc aacccagcat cccgtgccag tactgccgct tgctcattaa
     3361 tcacccccag ttgcatccag agggttttcg cgccaatggc aatagcttcc tgtgccacgc
     3421 cccacgccgc ttctgaatta cggaaaacat cgaccatatc gaccttttcc ggaacgtctg
     3481 ccagcgtgcc atacccctgc tggcccagca gcgttttgcc ggcaactttt ggcgaaaccg
     3541 gaatgacgtg atacccctgg tcgagtagat atttcatcac gcgatagctg ggacgatcgg
     3601 gtttgtcgct tgcccccacc agcgcaatgg tgtgagtgga cgttaaaatg cctgcaatat
     3661 cggtttcttt cattatttcc tccagactgt ttaacaaagt gtacgacaaa gccgacatgc
     3721 caaccatgca gctaatacga atgaatgaga gcaaaagcgg gaaatatgtc tatatgttag
     3781 taagtatgat taaagaaaaa ttttgacaca ttattattcg ggcggtcttt tgacaggagc
     3841 tacctatgaa aaccggcatc gtgaccacct tgattgcatt atgtttgccg gtatccgttt
     3901 ttgccaccac gctgcggctt tcaaccgatg tcgatcttct ggtactcgac ggcaagaagg
     3961 tttccagctc tctgctgcgc ggtgcggaca gcattgaact ggataatgga ccgcatcagt
     4021 tagtgtttcg cgttgagaag acgattcatc tttccaatag tgaagaacgg ctgtatatct
     4081 ccccaccgct ggtggtcagt tttaataccc agctcatcaa ccaggtcaat tttcgcctgc
     4141 ctcgcctgga gaatgagcgg gaagctaacc attttgatgc cgcgccgcgc cttgaattgt
     4201 tggatggcga tgcgacgccg attccggtaa agctggatat tctcgccatt acctcaacgg
     4261 caaaaacgat tgattatgag gtcgaggttg aacgctataa caaatccgcg aaacgcgctt
     4321 cactaccgca atttgccacg atgatggcag atgacagtac gctgctttcg ggtgtttccg
     4381 agctggatgc tattccaccg cagtctcagg tgctcacaga acaacggctg aagtattggt
     4441 ttaaactggc tgacccacaa acgcgaaata ctttcctgca gtgggcggaa aaacaaccat
     4501 cttcctgaga tttttgtgcc tgtgcgcagg ctttttcggt ctttatcttg cagcgataag
     4561 tgcttacagt aatctgtagg aaagttaact acggatgtac attatggaac tgacgactcg
     4621 cactttacct gcgcggaaac atattgcgct ggtggcacac gatcactgca aacaaatgct
     4681 gatgagctgg gtggaacggc atcaaccgtt actggaacaa cacgtactgt atgcaacagg
     4741 cactaccggt aacttgattt cccgtgcaac cggcatgaac gtcaacgcga tgttgagtgg
     4801 cccaatgggg ggtgaccagc aggttggcgc attgatctca gaagggaaaa ttgatgtatt
     4861 gattttcttc tgggatccac taaatgccgt gccgcacgat cctgacgtga aagccttgct
     4921 gcgtctggcg acggtatgga acattccggt cgccaccaac gtggcaacgg cagacttcat
     4981 tatccagtcg ccgcatttca acgacgcggt cgatattctg atccccgatt atcagcgtta
     5041 tctcgcggac cgtctgaagt aatattgcac aggtggcaaa cgccacctgt ttcttacggt
     5101 tttctcgcca ccggcacatc cagatttttc agtatttcca caaagggaga gggattctct
     5161 ttgttaaaca gtgcccatac ccgatggcgt gcccgggtca gcgccacgta cattaaccgc
     5221 cgttcttcag cgtccgggaa atcctcaacc ggtggcagta gcgcctcttc cataatcgac
     5281 tcccgcgccg cagccggaaa accatcactc ccctcctgca agccaacgat gatgacgtaa
     5341 tccgcctgtt gccctttgct ggcatgaatg gtcataaagt cgatttgcaa cttcggccag
     5401 cgtgttgccg ctttttccag gctggcaggc ctcatgtgat ggtaacgcgc caggataaga
     5461 atgcgctctt ccggtttggc ataaccagag agcttatcca gcaaagcgtc aagttgactc
     5521 tcatccaata acgtgacggc tttttcgtct ccattggtta agctgtttag cggctttttc
     5581 aactggcctg ggttctgctg aataaaccgg tttgccacct caccgatacg actgttaaaa
     5641 cggtaagtcg tgtctaaatc acagcgatcg ccttcaccaa agttttcatg gaaagcggtg
     5701 gtgagcgaca tttgcgcgcc gctgaatcgg taaatcgcct gccagtcatc accaacggcg
     5761 aacaacgtcg tctgactgtt ttgcttgcgt aatgccgcta acaacgctgc ccgctgcggc
     5821 gagatatcct gaaattcatc aaccagaata tgcttccacg ggctgataaa gcgacctttc
     5881 tccagaatca caatcgcctg atgaataagg cccgaaaaat cgacagcgtt ttctgccttc
     5941 agcgcacctt tccaggcttt taataacggg gccatcaact tgatacgttt actgaacaga
     6001 tcgcgaatct cttcgggtgc actggcaatc atttctgcct gtgcaccacc gtgcatccgc
     6061 atcagactta cccagcgatc gaggcgagag gcaaggcgac gctgtaattt ttcatcatcc
     6121 cagaagttac cttctggcac tgaccactgc atttcttccg tcagccattg ccgccagccc
     6181 ttcgcctgcg ctttcttttc gctgcattgc ttgcgccact cagcaataaa gagttcatga
     6241 cgggcagcgg tatcattttc cagtttgctg actatcggaa cttttttgct gccctgctga
     6301 ataatatgca gcgccagcgc atgaaacgtg cgtgcggtaa tgtcttcggt atgtagccgt
     6361 tcgcgaatcc gctcgtccat ctcttcagcg gctttgcgac caaacgccag caataaaatt
     6421 tgctcagggg acgcttcacc acgcgccagc aaccagcctg cacgggccac cagcaccgac
     6481 gtttttccgc ttcctgcacc tgccagcact aacagagaat gctcgccatt aacgactgcc
     6541 cgggcctgcg ccggattcag cggtgaagac tcgacctggc ggaaaaaatc cgcatactcg
     6601 gtaagcatgg cttcggtata cgcctggtta tgctgcaacc gagcgctttc aatatctttc
     6661 aaccaggcct gacatttacg ccacgcctca cggcagttat cgaattcttc cagtcggtta
     6721 accggcaacg gcaacgccga caaagcctgg cggatttgtt gctgcacgcc agaggtttgc
     6781 tcacgcgtca gccatttatt ttccccagtg cgcgtggcaa tcaaatccag ttgctggcgt
     6841 aaaacaccag acgcaatttc gctcatctcg ccactccacc gccgccagtg agcatcaaga
     6901 tgatggtaaa aacgctgcgt ctcgccccat tcggtgccgt gcagacgcac cactttttcg
     6961 tccggcagta caaattccag ctcgccccat accagaccgc gcttacagtg aatcgccagt
     7021 aattgattga aaggaataag gtattcgtgg cgatcgccgg agactttaat cccggcattg
     7081 aggatcaccg cccgatcgta agggtgctgt gccagacgtt ttccaagcgt tgtcgctttc
     7141 agttccatga ctctgtagat ccacacgaaa ttatttctta tcagtgtaac cgtcagagaa
     7201 aacgtgctcc agcccaaaaa aacgttacaa ttgccgccca ttactgaaaa ccacagtaaa
     7261 gcgaggtttt atgcgtaccg ttttgaacat tctgaacttt gtgcttggcg gatttgccac
     7321 cactctgggc tggctgttgg cgactctggt cagtattgtg ctgattttta ccttaccgct
     7381 gacacgatcc tgctgggaga tcactaaact gtctctggtg ccttatggca atgaagctat
     7441 tcatgtcgat gaactgaacc cggctggcaa aaatgtactg ctgaatactg gcggtacggt
     7501 attgaatatt ttctggctga ttttctttgg ctggtggtta tgcctgatgc acattgcaac
     7561 gggcatcgca caatgtattt caatcattgg cattcctgtc ggcattgcga actttaaaat
     7621 tgccgctatt gcactatggc cggttggtcg tcgcgtggta tcggtagaaa cagcgcaagc
     7681 tgcgcgtgaa gccaatgcac gtcgtcgttt tgaataatcc actaatatgg cctttatgct
     7741 aagtcctttg ctcaaacgct atacctggaa cagcgcctgg ctgtattacg cgcgtatttt
     7801 tattgcgctt tgtggaacca cagcgtttcc gtggtggctg ggtgatgtaa aactgacgat
     7861 tccgctaacg ctggggatgg tggcagcggc gctgaccgat ctcgatgacc gactggcggg
     7921 acgtttgcgt aacctcatca ttacgctgtt ctgctttttt atcgcctcgg cctcagtaga
     7981 attgctgttt ccctggccct ggctatttgc gattggctta acgctctcta ccagcggctt
     8041 cattttgctc ggcggtctgg gtcaacgcta tgcaacaatt gccttcggtg cattgctgat
     8101 cgccatttac actatgttgg gaacatcact gtatgagcac tggtatcagc agccgatgta
     8161 tctgctggcc ggtgccgtct ggtacaacgt cctgacactt attggtcatc tgctgttccc
     8221 ggtccgcccg ctgcaggaca acctggcgcg ttgctatgaa caactggcgc gttatcttga
     8281 gctcaagtcg cgcatgtttg atcctgatat tgaagatgaa agccaggcac cgctgtacga
     8341 tttggctctc gccaacggtc tgctgatggc gacattgaat cagacgaaac tctcgctgct
     8401 gacccgctta cgtggcgatc gtggtcaacg gggaacgcgt cgcacgctgc attattactt
     8461 tgtcgcacag gatattcacg agcgtgccag ctcttctcat attcagtatc aaacattgcg
     8521 tgaacatttt cgccacagcg acgtgctgtt ccgttttcag cggctgatgt cgatgcaggg
     8581 ccaggcgtgc cagcaactgt cacgctgtat tttgttgcgt cagccttatc aacatgatcc
     8641 gcattttgag cgcgctttta cgcatattga tgctgcgctg gagcggatgc gcgataacgg
     8701 cgcacccgcc gatttactca aaacactggg atttttgctg aacaatttac gcgccattga
     8761 tgcccaactg gcaacaattg aatcagaaca ggcccaggca ctaccccata ataatgacga
     8821 aaatgagctc gctgatgaca gcccgcacgg gttgagtgat atctggctgc gtcttagccg
     8881 tcacttcacg ccggaatccg ccctcttccg tcatgcggta agaatgtcgc tggtgttgtg
     8941 cttcggctac gccatcattc aaataaccgg aatgcatcac gggtattgga tcttgctgac
     9001 aagtttgttt gtctgccagc caaactataa cgccacgcgc caccgcctga agttaaggat
     9061 tattggtacg ctggtaggta tcgccattgg cattcctgtg ctgtggtttg tgccatcact
     9121 ggaagggcag ctggtgctgc tggttattac cggcgtgctc ttttttgcct tccgtaacgt
     9181 gcaatacgct catgcaacga tgttcatcac acttttggtg ctactgtgtt ttaacttact
     9241 gggtgaaggt tttgaagtag cgttacctcg cgtaatcgat acgctgattg gttgtgccat
     9301 tgcgtgggcg gcagtgagct acatctggcc tgactggcag tttcgcaatc tgccgcgcat
     9361 gctcgaacgc gccacagagg ccaactgtcg gtatctcgat gccatactgg agcaatacca
     9421 tcaggggcgt gataaccgtc tggcgtatcg tattgcccgc cgcgatgcac acaaccgtga
     9481 tgctgagctg gcgtcggtgg tatcaaatat gtccagcgag ccgaacgtta ccccgcaaat
     9541 tcgcgaagcc gcgtttcggt tgctgtgcct taaccatacg tttaccagct atatctcagc
     9601 cctcggtgct caccgggagc agttaactaa tcctgaaatt ctggcgtttc ttgatgacgc
     9661 agtttgctat gttgatgacg cgttacatca tcaacctgct gatgaagaac gcgtcaatga
     9721 ggcattagct agcctgaaac agcggatgca gcaacttgaa ccacgggcag acagcaaaga
     9781 acctctggtc gtacaacaag ttggattatt gattgcatta ctgcctgaga ttggtcgtct
     9841 gcaacgccag attactcaag ttccgcagga aactcctgtt tcggcgtaag agagtcagcc
     9901 cattctgcaa gctcctggcg gcgtgccacc gggagcgcag cttcatgaat gccgataatg
     9961 gcaccttcaa gcataaacag aatcttttct gtcaccagac tgttttgctg ccgcagtcgc
    10021 aaccaacaca tttttgcccc aagtatacgt aacgcccgta catccttaat ccccacctcc
    10081 ccgagaatcg cttccagatg aaaagacata ttgggcaaat ctttcagtct ttcccgggta
    10141 ttgcgcgtgc ttttctcttt cagcgctgca tcgagagaat atttcgacag acgcaccagc
    10201 ttcagttgat ttcgccatag actttcatca acccgatagt aattgagggt aacggatcgg
    10261 ccacactttt tatatgtcag ccagacaggc ggatgtttta cacagtactg tgcactttgc
    10321 tcacaagccc gaagatacaa ctcaccatca gaaaccatcg caaacaccgt gtcgtcaacg
    10381 gtcaggctgt aactgccaaa caatgatcgg tattcaattg tgcccaacgt tgccaggtat
    10441 tcttgtgatt tatagatccg cttataggag aggcttttca taaaattcct tttaaaatca
    10501 taacataaaa gaatgattca cattaacgga tccgttaact acgaaaatag gcaacttatt
    10561 cttaaggggc aagattaatt tatgttttcc cgtcaccaac gacaaaattt gcgaggctct
    10621 ttccgaaaat agggttgatc tttgttgtca ctggatgtac tgtacatcca tacagtaact
    10681 cacaggggct ggattgatta tgtacacttc aggctatgca catcgttctt cgtcgttctc
    10741 atccgcagca agtaaaattg cgcgtgtctc tacggaaaac actacagccg ggcttatcag
    10801 tgaagttgtc tatcgcgaag atcagcccat gatgacgcaa cttctactgt tgccattgtt
    10861 acagcaactc ggtcagcaat cgcgctggca actctggtta acaccgcaac aaaaactgag
    10921 tcgggaatgg gttcaggcat ctgggctacc cttaacgaaa gtaatgcaga ttagccagct
    10981 ctccccttgc cacactgtgg agtcaatggt tcgcgcttta cgcacgggca attacagtgt
    11041 ggtgatcggt tggttggcag atgatttgac tgaagaagag catgctgaac ttgttgatgc
    11101 ggcaaatgaa ggtaacgcta tggggtttat tatgcgtccg gtaagcgcat cctctcacgc
    11161 cacgagacaa ctttccgggc taaaaattca ctctaatttg tatcattaag taaatttagg
    11221 attaatcctg gaactttttt tgtcgcccag ccaatgcttt cagtcgtgac taattttcct
    11281 tgcggaggct tgtctgaagc ggtttccgcg attttcttct gtaaattgtc gctgacaaaa
    11341 aagattaaac ataccttata caagactttt ttttcatatg cctgacggag ttcacacttg
    11401 taagttttca actacgttgt agactttaca tcgccagggg tgctcggcat aagccgaaga
    11461 tatcggtaga gttaatattg agcagatccc ccggtgaagg atttaaccgt gttatctcgt
    11521 tggagatatt catggcgtat tttggatgat aacgaggcgc aaaaaatgaa aaagacagct
    11581 atcgcgattg cagtggcact ggctggtttc gctaccgtag cgcaggccgc tccgaaagat
    11641 aacacctggt acactggtgc taaactgggc tggtcccagt accatgacac tggtttcatc
    11701 aacaacaatg gcccgaccca tgaaaaccaa ctgggcgctg gtgcttttgg tggttaccag
    11761 gttaacccgt atgttggctt tgaaatgggt tacgactggt taggtcgtat gccgtacaaa
    11821 ggcagcgttg aaaacggtgc atacaaagct cagggcgttc aactgaccgc taaactgggt
    11881 tacccaatca ctgacgacct ggacatctac actcgtctgg gtggtatggt atggcgtgca
    11941 gacactaaat ccaacgttta tggtaaaaac cacgacaccg gcgtttctcc ggtcttcgct
    12001 ggcggtgttg agtacgcgat cactcctgaa atcgctaccc gtctggaata ccagtggacc
    12061 aacaacatcg gtgacgcaca caccatcggc actcgtccgg acaacggcat gctgagcctg
    12121 ggtgtttcct accgtttcgg tcagggcgaa gcagctccag tagttgctcc ggctccagct
    12181 ccggcaccgg aagtacagac caagcacttc actctgaagt ctgacgttct gttcaacttc
    12241 aacaaagcaa ccctgaaacc ggaaggtcag gctgctctgg atcagctgta cagccagctg
    12301 agcaacctgg atccgaaaga cggttccgta gttgttctgg gttacaccga ccgcatcggt
    12361 tctgacgctt acaaccaggg tctgtccgag cgccgtgctc agtctgttgt tgattacctg
    12421 atctccaaag gtatcccggc agacaagatc tccgcacgtg gtatgggcga atccaacccg
    12481 gttactggca acacctgtga caacgtgaaa cagcgtgctg cactgatcga ctgcctggct
    12541 ccggatcgtc gcgtagagat cgaagttaaa ggtatcaaag acgttgtaac tcagccgcaa
    12601 gcttaagttc tcgtctggta gaaaaacccc gctgctgcgg ggtttttttg cctttagtaa
    12661 attgaactga ctttcgtcag ttattcctta cccagcaatg cctgcagatc ctgcttcaga
    12721 gaagacattt tattcgcgta tttctctttg ttttccgcat cttcaatcag ctgaacaatc
    12781 gtttcagaaa gcgttttacc gcgacgctgc gcaagaccag ccagacgttg ccagacaata
    12841 aattccagat cgatcgattt tttgcgcgta tgctggtgtt ccgcattaaa atggcgcttt
    12901 cgtcttgccc gaatggtttg cttcatgcga ttgaccagtt ccggattcat atgcttgtca
    12961 atccagccat ttaccagcac gggttcattt tcgagcgaga gcaacacatc gacggcttcc
    13021 tgggcggcac tggcttctat gtaacgggtg attaactccc cttcgcgatg cttcttcacc
    13081 agatacttcc atttccaacc gctttcaaga ttttcaagtt gttgatattt catagcgatc
    13141 tcaatgttac cgtgtaactc tttacagaat atcagctttt taggccagtg aagaaaagaa
    13201 tctccatcct gtgagcttta acgcccatgc caggagtatt gtcggtgctt tctacgtgtg
    13261 ctgtagtgcc ggttacggta taatcgcggc tttgacaaca gactaaaaaa catcaacttt
    13321 gaccattacg aaacttgcat ggcgtgacct ggttcctgat accgatagct atcaggaaat
    13381 atttgctcag ccacatttga ttgacgaaaa cgatccttta ttcagtgata ctcaaccgcg
    13441 actgcaattc gcactggagc agttgctgca tacgcgagca tcctcctctt ttatgctggc
    13501 gaaggccccg gaagagtctg agtatctgaa tcttattgcc aatgccgcgc gtacgctaca
    13561 aagcgatgca ggccaactgg tgggcggtca ctatgaggtt tccggccact ccatccgctt
    13621 acgtcacgca gtgagtgcag atgataattt tgcgacttta acgcaagttg tcgctgccga
    13681 ctgggtagaa gcggagcaac tctttggctg cctgcgccag tttaatggcg acattaccct
    13741 gcagcctggt ctggtgcatc aggcaaatgg cggtattctc atcatctctt tgcgtacact
    13801 gctggcgcaa cctctgctgt ggatgcggct gaaaaatatc gttaaccgcg agcgttttga
    13861 ctgggttgcg tttgatgagt cgcgccctct ccccgtctct gtgccttcga tgccattgaa
    13921 gctgaaagtc attctggtag gcgaacgcga atcattgtct gatttccagg agatggagcc
    13981 agagctttca gagcaggcta tttatagcga atttgaagat actctgcaga ttgtcgatgc
    14041 agagtcagta acccagtggt gtcgctgggt gacatttacc gccagacata atcacttacc
    14101 tgcaccagga gcggatgcct ggccggtact tatccgcgaa gcagcacgct acaccggtga
    14161 acaagaaaca cttccgctta gcccgcagtg gatcctccgc cagtgtaaag aggtcgcctc
    14221 cctgtgtgat ggcgacacct tctccggcga gcagctaaac ttaatgctgc agcagcgtga
    14281 atggcgcgaa ggtttcctcg ctgaacgtat gcaggatgag atccttcagg agcaaatcct
    14341 gattgaaacc gaaggcgaac gcatcgggca aattaacgcc ctttcggtca ttgaatttcc
    14401 gggtcatcca cgcgcttttg gcgaaccttc tcgcattagc tgcgttgtgc atattggcga
    14461 tggtgaattc accgacatcg aacgcaaagc ggagcttggc ggcaatatcc atgcgaaagg
    14521 gatgatgatc atgcaagcgt tcctgatgtc ggaactacag cttgagcaac agatcccctt
    14581 ctcagcatcg ctgacatttg agcagtcata cagtgaagtt gatggagata gtgcctcgat
    14641 ggctgaactc tgcgccctga taagcgccct cgccgatgtg ccggtgaatc agagtatcgc
    14701 tatcacaggt tcagtcgatc agttcggtcg cgcccagccg gtcggtggtt taaatgagaa
    14761 aatcgaaggc ttctttgcta tttgccagca acgtgagtta accgggaaac aaggtgtcat
    14821 tatccccaca gctaacgttc gccatttaag tcttcacagt gaactggtga aagcggtaga
    14881 agaaggcaaa ttcaccatct gggcagtaga cgatgtgact gacgcactgc cgttattatt
    14941 aaatctggtg tgggatggcg aaggccaaac gacgctgatg caaaccatcc aggaacgtat
    15001 cgcgcaagca tcgcaacagg aaggacgtca ccgttttcca tggccattac gttggctgaa
    15061 ctggtttatt ccgaactgat cggacttgtt cagcgtacac gtgttagcta tcctgcgtgc
    15121 ttcaataaaa taaggcttac agagaacatg gtagataaac gcgaatccta tacaaaagaa
    15181 gaccttcttg cctctggtcg cggtgaactg tttggcgcta aaggcccgca attgccagca
    15241 ccgaacatgc tgatgatgga ccgtgtggtc aaaatgaccg aaacgggtgg taacttcgac
    15301 aaagggtatg ttgaagcaga actggatatc aatccggatc tgtggttctt cggatgccac
    15361 tttattggcg atccggttat gccgggatgc ctgggcctgg acgcaatgtg gcagctggta
    15421 gggttctacc tcggctggct gggcggcgaa ggtaaaggcc gcgcgctggg cgttggcgaa
    15481 gtgaaattca ctggtcaggt actgccgaca gcgaaaaaag tgacctaccg tattcacttt
    15541 aaacgcattg ttaaccgtcg tctgattatg ggcctggcgg atggcgaagt gctggttgat
    15601 ggtcgtctga tctataccgc cagcgacctg aaagtcggtc tgttccagga tacgtctgcc
    15661 ttctgattat aatcagacag agtatcaaaa ggcgaaacct ccgcaatgcg gaggtttctt
    15721 tttaaagaga cagaatcagg ccattactac cctgtccgcc atggcttctc gccagcctcc
    15781 cagccattgt gacctttgat tcagcgtctg atagggacac atttcttttg agcgtccggc
    15841 gatgccggcc tgataaccac gttgatgtgc ccgttccagg cgatctcgtt tttgtctctt
    15901 catgcctcgt ttccctcata ctggtttctg gtggaaaaga aaacagtgat tactaaatgt
    15961 gcaatcacag ttaagcaata ccgcgaatca taactgccgt caatgcgcaa aattcacgcc
    16021 actgtcatat ttgtgagcta cacgggtatg ccggttgtac aaaaaatgtg aagaggaaca
    16081 actttttccg cacgttagag acaaaaaaac cgcctcaggc aacataccgg aggcggttat
    16141 atttacaaat ctttataatt aaggtagacg ctttatctct tgcgcaatag aagcggcttc
    16201 ctgactccag gcactggcca gtactttaac catctcatcg taaccatcct gagtttgcac
    16261 cccttccaga cggaacggac gtttgatcag ttgtccctgg tggttcaaca gccactcacc
    16321 actgacaatg accttgccat catagcgacc gttaaactcc gtgacggtaa cattgagcgt
    16381 gtcctgggcg cttcccagag gctgggaggc aaccacccag ccgggcagtt gcgtactcag
    16441 gttggcaacc agggtgttgc gcaactgttg atccaacggg ctggcccaca agttgttatt
    16501 ggcaatcaca tacttcacat cactggtttg ataaaccaca ccattccccg ccagatagtc
    16561 aggaacagtg acctgctcta cccataacag acgattgccc tggctggcgg tactttgtgt
    16621 accgctctgc accacaggta actggtaata gtttttatta atttcgccgg agctgcatcc
    16681 ggccagccac agtgctgcaa tcgtcactag ccactttttc attgtttcgc cctcttcggc
    16741 tctggatctt ttttgtcctt cgcttcaaat accagcgcgt tactcttctc attgagcgtt
    16801 ttcagcaccg gttgcagttc tcgcaacacc tgatcaaggc gctgcatatc cgccaccatc
    16861 ttgttgtagg ctgcggagcc aggctggaag ccctgcatgc tgcgattcaa ttcacgcaac
    16921 gttgactgca tatccgtcgg caactgctgc atcgactggc tagcgaggat cttgttcatg
    16981 ctatccagcg tcgtttgcag gtttttcatt gtgcgctgac tttcagaaag cgtactggtt
    17041 gcctgttcaa tcatcggatt caatggcagt ttgttgatct tatccaacgc ttccatcagt
    17101 cgttgctgga tttgcgccag gccgccgcta acggtcggga taatctgata accattaaat
    17161 tcacgaatac cggttattgc aggcgtattt ggatagaaat cgagatcaac atacagtgca
    17221 ccagtgacca ggtttccggt tttcagcgat ccgcgtaaac cacgtttcaa caattcgcca
    17281 aggtgctcaa caacatccgc attttcgcca agctgcattt tcagccgctc tggctcgata
    17341 cgaatcagta ccggaatacg gtaatcatcg ttaaatgtct gacgcatatt cggcgcaaag
    17401 aatggcactt tgcttacggt acccaggcga ataccccgga actctaccgg agctcccggt
    17461 tgcagaccgc gtaccgaatc tttaaagaac atcagataat caatgtgatc ggtgtacaac
    17521 gaatcctgaa tgctcttctg atcatcatac aaaacgaaag ctgttttcgg tgccactggc
    17581 tgccctaaat ccagaccttc cggcacatca aagctgacac cgccactcag cagcgttgtc
    17641 aatgagccca tctccacacg catccctgct gacgtcagat caaccgcaat gccactatct
    17701 ttccagaagc gaacattgtt ggtcaccagt cggtcataag gtgcattgat gaacagttga
    17761 tagctgatat tgcgtttttg tgtatcgaag gtgctggttt caaccgaacc tacccgatag
    17821 ccacggaaca gcaccggatc tcctggcgag agctgcccgg cttttttgct atcgagaatc
    17881 acacggatac ctttcgcatc aggcggggcc aacggtggcg agtccagcaa atcgtattta
    17941 tccattttgc tgcctttcgc gcctggctgc agttcgatat aaaccccaga cagcagggtt
    18001 cccagaccgc taatcccttc tcgaccaatc tgcggtttca ccacccaaaa gacggtgtct
    18061 ttatgcagca atttttccat accggaattc agccgcgctt tgatttcaac gtgcgtcaaa
    18121 tcatcagcca gtgtggcgct ttcaaccacg ccgacgtcaa cgctacggct tttaatggtg
    18181 gttttgccac cttcaattcc ttccgcattc gcggtgatca gggtcacttc cggtccctga
    18241 tggctgtaat gataaaaaag aacccaggcc ccaatgagcg ccgtgacgat aggaaatatc
    18301 cacacgggag accagttctt cactttctgg attttggctt ccccattatt agattccata
    18361 ctgtcaggac tcctcatgct ctgattcagg ttgacgatcc cacgacaaac gcgggtcaaa
    18421 cgtcatagca gaaaacattg tcattatgac gactaaagca aacattaatg cacccattgc
    18481 cggataaata ctcattaaac ctcccatacg caccagcgcc gagagcaccg cgataacgaa
    18541 aacgtcaatc atcgaccagc ggcctacaaa ctcaacaact tcataaatca aatgcattct
    18601 ttcactgtcg cgcttgccat gccctttggc atcccaacac agccacgcga tggcgatcat
    18661 ctttaacgtt ggcaccataa tactggccag aaagatcacc gcagcgacgg gataagagcc
    18721 ttcgctccat aacagaatga ccccagcgag aatcgtcgac ggcatcttcg agcctaataa
    18781 atccgtcacc atgatgggca aaatattagc cggaagatac agcatgatgg acgttacaag
    18841 cagcgcgagt gtccactgca ggctgtttct acgccgaacg taccctttgg tactacaacg
    18901 cgggcacacg ggttcatcag caggaaggat tgccgtacaa caggagcacg aacgcagccc
    18961 ctgacgtatc cccgtgacgc ctggttttag cggctggcgc agttctggca tcggggcgat
    19021 gtcgtcccat aaccagcgac gatcaacgca ctgaaaagcg cgcagttgca ggacacaaaa
    19081 taaacaccag gggagaaagc tgctgcctac cccaatgctg ccgtaagcca tcagtttaac
    19141 gaaactgacc agcacaccag cgaggaaaat ctccgccatt ccccaggttt tgagttgaaa
    19201 aagcacccgt gccagttgct cttttaaacg gaccggtaat tccgcgcgat tcaccagtaa
    19261 cagaatggtt atcagacaaa acgcgggaac cagttgcaca aacaacagga aaaaggtgcc
    19321 gaggctggcg tagtcctcag aaaaaagcac gccgggaatt tccagtaatg taatttcact
    19381 ggtaactcct gcaacgttca tattcacaaa aggaaacaag ttggacagca acagcatgaa
    19441 cagtgcagcc aacgcatagg cggtgggacg ctgccgaggg gcatcccacg ccacggttaa
    19501 cgttgtgcca caccggggac atgccgcttt ctgaccatgc tcaaggcgcg gtaacgccac
    19561 cagcatgtca cactgcgagc acaggatgtg cttcgcggca tgatgatgtt cgcacattgg
    19621 tactccttaa tgatgcgcta ttttacagat gactaacaaa gtgttggttt tcatgcctga
    19681 taaacccagt tcatcaggca attttaccgt tgtcagctgt ctctgtttaa atcgactatt
    19741 ttgcgatcag ccaccatttt ttaacgcttc aagatactcc cagcgttcaa aggcttgctc
    19801 cagctcctgc tctgcagcag ccatatcggc gagcactttt tgtgtctgct catgtggctg
    19861 actgaagaag gaagcatccg ccacctgcgt ctgtagggct tccagcttcg cctccagatc
    19921 ttcgagcaat tgcggtagct gctccagttc gcgctgcaat ttatagctta gtttgctact
    19981 gctgcgtttt acagtttctg cttttgccgc agcggcttct tcggtttttt tcaccgcagg
    20041 ctgtttgagc gccacatact gctcttgctg accacgggca tcatgataac cgccgacata
    20101 acgaccaatt ttaccgccgc cttcgaagat ccaacattct gtaacggtgt tatcgacaaa
    20161 ctgacgatcg tggctaacca gcaataccgt gccctgatag ctgtcgatca gttcttccag
    20221 cagttccagc gtttcgacat caagatcgtt ggtcggttcg tcaagaatca ataagttgct
    20281 tggtttgagg aacaaacgcg ccagcagcaa gcggttccgc tcaccgccag aaagcgcacg
    20341 taccggcgtc atcgcccgtt tcggatggaa cagaaagtcc tgcaaatagc ccaatacgtg
    20401 gcgtggcttg ccgttaacca tcacctcttg cttaccttcg gcaaggttat ccatcaccgt
    20461 tttatcggga tccagttccg cgcggtgctg atcgaaataa gccacttcca gtttggtgcc
    20521 aacgtgaata cgcccgctgt ccgcttgaag ctgaccgagc atcagtttca gcagcgtggt
    20581 tttgccgcac ccattcggac caatcagggc aattttgtcg ccacgtagaa cctgggccga
    20641 aaaatctttc accagttgct taccgtcaac ctggtagcaa acgtcttcca tttcgaaaac
    20701 gatcttaccg gagcggctgg cctcttccac ctgcatcttt gcggtaccca tcacttcgcg
    20761 acgttcacca cgctcgcgac gcattgcttt cagggcgcgt acgcggcctt cattacgggt
    20821 acggcgtgct ttgatcccct ggcgaatcca cacttcttcc tgcgccagtt tgcgatcgaa
    20881 ctcggcattt tgtagttctt ccacgcgcag ggcttcttct ttttcctgca ggtactggtc
    20941 gtaattcccc ggataggtca ccagcttgcc gcgatcgaga tcaacaatgc gcgtcgccat
    21001 attgcggata aacgaacggt cgtgggagat gaaaataatc gtcccattga aggttttaag
    21061 gaacccttcc agccagtcga tggtttcgat atccagatgg ttagtcggtt cgtccagcag
    21121 caatacgcgc ggattgctca ccagcgcgcg tcctaatgcc gctttacgca accagccgcc
    21181 ggaaagcgac gacagcgcaa cattaggatc taaccccagt tgcgccagca cttcgttgat
    21241 gcggttttcc agctgccaca agttgtggtg atccagctgt tcctgaacct tcgccagttc
    21301 gttgagattt ttctcgctcg ggtcgttcat caccaggcgc gaaatatcgt gatagcgttt
    21361 cagatattcc gcttgttctt caatgccttc ggcaacgaaa tcataaacgc taccctcaac
    21421 gttacgcggc ggatcctgtt gcagacgagc tacaatcaaa tcttgctcgt aaataatgcg
    21481 accgtcatcc agcccttgtt cacggttgag gattttcatt aacgtcgatt tgcctgcgcc
    21541 gttgcggcct accagacaaa cacgttcatt atcttcgata tgcagttctg cgttatcgag
    21601 aagcggcgcg tcgctgaacg acagccaggc gccatgcata ctgattaatg acattactat
    21661 tcctttcagg ctgcggtaat cagccagcag ttgtggatct ggcggttacg ggcgaaatcc
    21721 tgggagagcg ttttttgcgt aatttcttgt gctttcagtc ccagttttgc caggccgccg
    21781 agatccatac ggaagccacg tttgttgttc gagaacatga tcgtcccacc tgcacgcagc
    21841 agacgtttca aatctttcat cagcgccaga tgatcgcgct gaacatcaaa cgcatcttcc
    21901 attcgttttg agttagagaa ggttggcgga tcgataaaga tcagatcgaa ctgttcattt
    21961 gcctcacgca gccacgccag gcaatcggcc tgaatcaggc gatgcgcacg cccggtcagg
    22021 ccattcagac gcaggttgcg ttctgcccac tccagataag tacgcgacat atccacggtg
    22081 gtggtgctgc gtgcaccgcc taatcccgca tgcacggtgg cgctgccggt ataagagaac
    22141 aggttgagga aatctttgcc tttgctcatc tggccgagca tacgacgggc gatacggtga
    22201 tcgagaaaca gaccggtatc gaggtaatcc gtcaggttca ccaacaagtg agcgttatat
    22261 tcggtaactt caagaaactc gcccttctcg cccagtttct ggtactgatt tttgcccttc
    22321 tggcgttcac gggttttcag caccagtttg tttggcgcaa tccccagtac cgaaatggtt
    22381 gcagcgataa tatcgaacag acgctgacgc gctttgtgag catcaatagt ttttggcggc
    22441 gcatactcct gcaccaccac ccagtcggca taacggtcaa cggcaacgtt atattccggt
    22501 agatcggcgt catacaggcg gtaacattca attccttcct ggcgagccca cttctcgaat
    22561 tttttgaggt tcttacgcag gcggttggcg tagtcttccg ctaccatcgc cggtttgctg
    22621 tctggagtgc tttcggcaac gtggtaattt ttctgtacgc agtccagcgg gccgtttttc
    22681 gccttgtact gtttgtctgc acgcagctgc aagcagctta gcagatccgg cgaggcacta
    22741 aacaaagaga gattccagcc accaaactgg tttttcatga tccgacccag caggctatgc
    22801 agcgcaatca gcgccggttc gctgtccaga cgttccccgt atggcgggtt gctcaacact
    22861 gtgccgtacg gccctttcgg cagcggattg gtcagttgcg cgacatcttt cacctcaaag
    22921 gtgatcagtt caccaatccc cgcaagacgg gcgttagtgc gtgcacgttg aatcacccgt
    22981 gcgtcgctgt ccgaaccgta aaaatgagag ctatactcag ccaggccttt acgggcgcga
    23041 gtttgcgctt ccgctttcac ttcctgccag atagcttcat catgctgcgc ccagccgcta
    23101 aagccccaac gcccacggtg caagcctggt gcgcgatcgg tcgccagcat cgccgcttca
    23161 atcagcaacg taccggaacc acacatcgga tcgagcagcg gtgttcctgg ctgccagccg
    23221 gatcgcatca caatagcggc tgccagggtt tctttgatcg gcgcaatacc agcacgatcg
    23281 cgatagccac gcagatgtaa accatcacca ctcagatcaa gagcgatact ggcggtttct
    23341 ttatgcagcc agacgttaac gcggatatcc ggcgcatcgc gatcaacatt tggacgcggc
    23401 agatttttcc gcgtgaaagc atcgacgatc gcgtctttca ctttcatcgc gccgtactga
    23461 ctgttgcgga tggtgtcatt caaaccactg aagtggacag cgaaggtcgc gccaggatta
    23521 aacatctctg tccagttgat cgcctgaaca ccgagataga ggtctaaatc gctgtaaacc
    23581 ttacactcgc ccagcggcaa cataatacgc gaggccaggc ggctccacat caggctctgg
    23641 taaacaagcc gtgtgtcgcc cttgaaatgg accccaccct gaaccacctg gcattcaacg
    23701 gcccccaggt tttccagttc agtttttaac agctcttcca gcccacgggc cgtactggca
    23761 aacagagaat tcatatcgtc acttttactc taagagccta ccgcagtagg cgtaattgtt
    23821 gcagtcagtc tggacatgga cagcgcggag aaaccggagc gtacacaagt acgtgaggat
    23881 ttcgagcact tccctgggcc aaactgacaa ataaaatagc ctaacgggac aggcgctaag
    23941 aaaattgttg cgcattatag ctaataagcg cgtcatgtca taaagttgag ggcttatttt
    24001 catttgagga ccgcaccgtg gcgacattaa cccggctttt tattcatcct gttaaatcga
    24061 tgcgcggcat tggtcttaca cataccctgg cagatgtgag tggtctggcc ttcgatcgca
    24121 tctttatgat cacggaacct gatggtacgt ttattactgc tcgccagttt ccgcaaatgg
    24181 tacggtttac tccttcacct gtgcatgatg gcttgcatct caccgcacca gatggcagta
    24241 gcgcatatgt tcgttttgct gatttcgcca cacaagacgc accaaccgaa gtttggggca
    24301 cacattttac cgcgcgaatt gcgccaggcg cgatcaacaa atggctaagt ggatttttct
    24361 cccgcgaagt gcaattacgc tgggtggggc cacaaatgac ccggcgcgtg aaacgccaca
    24421 acactgtacc tctgtcattt gctgatggct atccttacct tcttgctaac gaagcctcgt
    24481 tacgtgatct ccaacaacgt tgtccggcca gtgtaaaaat ggagcaattc cgccccaatc
    24541 tggtggtttc cggcgcgtca gcctgggaag aagatcgctg gaaagtgatt cgcattggtg
    24601 atgtggtgtt tgatgtggtt aaaccttgta gccgctgtat tttcaccacc gtcagcccag
    24661 aaaaagggca aaaacatccg gcaggcgaac cattaaaaac attgcaatct ttccgcactg
    24721 cccaggataa cggcgatgtc gattttggtc agaatttaat tgcccgtaat agcggcgtga
    24781 ttcgcgttgg cgatgaggtg gaaattctgg caacggctcc ggcaaaaatt tacggcgcag
    24841 ctgccgctga tgataccgcc aacatcacgc aacaaccgga cgcaaatgta gatattgact
    24901 ggcagggaca ggcatttcgt ggaaataacc aacaggtgtt gctggagcaa ttagaaaatc
    24961 agggaattcg tatcccttat tcttgccgcg cgggcatttg tggaagttgc cgtgttcagc
    25021 ttttagaagg cgaagtcacg ccgctaaaaa aatcagcaat gggcgatgat ggcaccattc
    25081 tttgctgtag ctgtgtaccg aagactgcac ttaagttggc gcgttagact gcctgttcga
    25141 ggctgaagct gtcaacatta acctgcggtt tcagcctgtc gttcatgatt ttaatggcgt
    25201 cgcccaactg catcgcgcga cccgcaataa caacgcaggg ttgtgccagc aggcatagtg
    25261 cggcattttc gccagattca acgaccagca aattgacctg ctcgtgagta tcgctcaacc
    25321 aaacgcaggc cgcatcgccc ggcatgggca cccacatttc accatgggaa acaaaatgcc
    25381 agcttttcgg catttgcggt ttgagatagc gaattgctac cagtgcattc agcaccagtt
    25441 cggctttttg ttctttagat aaattaaaat cgcggcactt ttcttcaaaa gagaaataga
    25501 gcgcggcgtc gtcaacgcaa aagcctgcgg gagaaaaggc atctggagtc aacatcttgc
    25561 gcgcaaaacg tgagcgaaat agcataccat tggctaaatc gagcatcatt cgatcgtgct
    25621 cttcatcgta ataccaacgc cagttatcgt ctggtttaat tcgcatctgc ctctccccta
    25681 ccctataaaa ccgcagcaac acaatccatt gtcgcttcgc ttaataacct caacaataaa
    25741 agtacaaaat gtctaaataa gcaacagcga tgaatataaa acaaccaggg ctgaaaataa
    25801 agccctgatt atcttataaa gaggaattcg aagaaataat tagatatggg taacgatttc
    25861 tttaatcagc ggcggacctt taaaaataaa accagaataa atttgcacca gtgaggcacc
    25921 cgcagcaatc ttttcacgcg cagcgataac cgagtcgatg ccgccaacac cgatgatcgg
    25981 taagcgaccg tttaattcca gtgacaagcg gcgaataatt tcggtgcttt ttaactgaag
    26041 cggacgacca cttaagccac cggtttgatc gcaatttttc attccctgaa caagagaacg
    26101 atcgagtgtg gtattggttg caataacgcc atcaatatta tggcgaacta aactatcggc
    26161 aacctggatc aattcttctt cagaaagatc cggcgcgatc ttcactgcga tcggcacata
    26221 tttatggtgc atcgcttgca aatcattttg cttattttta atcgcggtta agagatcatc
    26281 cagcgcttca ccatattgca gcgtgcgtaa tcctggggta ttcggcgatg aaatattgat
    26341 ggcgatatat cccgcatagg catagatttt ttccatacaa atcagatagt catctttgcc
    26401 ctgctccact ggcgtatctt tatttttgcc gatgttaata cccaggacgc cgtcataatg
    26461 ggcctttttt acgttctcta cgaggttatc aacgccaaga ttattaaagc ccatacggtt
    26521 gatcaaacct tcggcatcta ccagacgaaa gagacgcggc ttgtcattac ctggctgtgg
    26581 acgtggcgtg acggtaccga tctcgatcga tccaaatccc atcgcgccta acgcgtcaat
    26641 gcactccccg tctttatcaa gaccggctgc cagaccaagc ggatttttaa acgtcaggcc
    26701 catgcagtta acaggtttcg caggcacttt ctgccgcacc agtgcttcaa acggcgttcc
    26761 tgtaatacgg cgtaattgct gaaaagtaaa ctcatgagcg cgctctggat cgagctggaa
    26821 aagggcttta cgaacgaagg ggtagtacat gaactctcct ggattcccgg tgtgcaaacc
    26881 gggggcgtat tatgtgcgag ttcggaccaa aagggaattg acctgcggca aaaaaaacgc
    26941 aaacgttttc cgaacctgtt tcagggcaga ttttgctcct tctttgcact caacccgccg
    27001 taatcattta tcgcccgcca ggcgacatga cacggtgcga ttgcctgaca ccagcttgtc
    27061 tgacgctggc ttttaggcgg cacaactccc gcattactta acgctttacc atttacgctc
    27121 aactgaatca ggttaatgta gtaaggcgtt gggttagtga gttgtacacc ctgcgaattc
    27181 cgtgtccagc gtaattgttg ataagcttcc agcgggtcgc ccggcaaacc ttcgggtcgc
    27241 cagaacagtt taaataccga gcgcatcgcc acttttacgc tctgattttc aactttgccg
    27301 gatggcacgc tggcaatgct taactcaaat agcgtttcgc gatccacagg caagatgtcg
    27361 ctttccgttc tcagcaagcg caacgtgcct gtagtaccgg gcttcaggag aataagtggc
    27421 ggagcggcca gtaatggtgc tggcactgtc gacgcttccc ccccggccca acgcgttggg
    27481 cggttgattt tactgttaat aagccaggat tcctgactgg tattagtcag taaaatagat
    27541 atcgattctc tgtctgccgg aaagataaat cgcgttccgc cgaccaccac tcctgcccgc
    27601 aattcacccg ccaccagcag cgataaaacg gcgaaaatca agagcgccaa tctgttccac
    27661 gtattagtca taatgaagta caaatgtcgc gctggcattc actttacccg actgcacatc
    27721 gctattgaca ggtcgcaatt gcgcataaaa agttaatggc accttaccgc tatgtaggct
    27781 gtagtcttta ctcgcctggt taagcggaat caatatcttc gcatcatcca gaatcgcaat
    27841 tgccagcccg ctggctgcat ccataccact ttcaatggac agcatttcgc tctgcactcc
    27901 gtcgggggtt cctgaaaagg tgacgtcaac cgtgctgaca tctgttccgc aatcctgcaa
    27961 attaatgatg aaagatttct ccgggccata attcccgtga gaaaaacgtg tcagcgagac
    28021 atcgcctaac tgcacattga tttgcttgct atcctgtgcc agcacgcatg tattacgacg
    28081 aaacgatccg ctaatgttga ccgtgccatc atgagcaaat aactcactac tccccgttaa
    28141 tcccagtaag cacagaatcc agattatccg tttcaacatc actccttcac tggtaaacca
    28201 gatcaaaata gagaaccgcc gtcgcattac cgcccgttgc tcccgccttt gtggacttat
    28261 aacgaagttg atatttgagt gtgttatccc cggcttttaa gggcgtaaga ggctggacct
    28321 gattgagcgg gatttcttgc tgactttgcg catcaagaat ttgcacggca atccccgttg
    28381 ccataccacc gcttccgcct gtgtcagtta gtttaagcag tgtcggcgca agggtgtcag
    28441 cctcgccgct aaaaaggacg tccgcgccag taataccagc ggcacaaccg gtgatatcga
    28501 tattcaaatc agctgcagtg cttacggtat tcgcggcctg aaaatcactg atattaaaat
    28561 cgccaatatg aatattttgt aatgcacttt tggtggcgac atcacagccc tgatcgacca
    28621 cattcccata aatatgcata ctgacttgtt caggcccagc catcccgaca tggatgttaa
    28681 gaaggctaaa gaacaaaata acgtactgat atatcgtttt tttcttcata cgttcacgcg
    28741 ttaatcaaaa tttattctca ccgttgccgt ggcttcaaat cgcccggttt ctggtggtac
    28801 gcctgtcgtg ctgactggat agacattcat tcgcgcgata ctgtctgcat catcaggaat
    28861 agagagcggt agtgtcccac cagcagggga aataatgttg ttctggctat cggtcaccac
    28921 cacgcccacg ccaggacgtg acgtcttaat cgcctgggga tagctggggt ctgaggttgc
    28981 cgtcagactc aggttgacgt taactaaccc ttcgtttccc gtgttacacc tgactggaat
    29041 tgcgagttct gccgttctgg ccccaagggg tttatgccct acaacacgaa agttagccgc
    29101 aaatacgtcg cccagatcga cctcgatagt ctctccggca ttgacggaac atgagccaag
    29161 tgaattgatc gtaccagaca ggtaatacac taaaaccggt gtacctgtag tcgtgcagga
    29221 atcacctgcg ggtatgttgt agcattcaaa taatcgcgcc agcgccatag gaggaataac
    29281 gaccgaacca acgaacggct gcaatatttt cagggaaaag ctaccgctat taccgctagc
    29341 cccctggcca cctaaacgtt gaccgccggg atcgcaaata tgccatcgtg tatctgcccc
    29401 tgtgcccacg ttttcgaaag gaaccgcagt aggagaactg cctttaacct gcaataaaac
    29461 tttggcggca agatagtcat taattttgta ccagttgtcg cctaaataga ctaacggtga
    29521 atccgcggcg aatgcccatc ggccacttct gacattgtct ttatcgcaat cacaggaaac
    29581 ataataatca ttcgattcgt tccactgata aaatttgtct ttaactactc cagccgtatt
    29641 ttcttcttcc tccagggttt taacgccaaa attgacgcca atatacggat tcccctgagt
    29701 ggtatgacac tgcccgctat aagcagcagt ttgagcgcac catagcatta agacggcggc
    29761 aaaaaatagt cgtagtaata acacgcattt ttctccaaag attatctgca tatggcatcc
    29821 attcgcgtaa tcgcagagtt aatttgttgt tcggtaagct ggtaactgat atgacaaatt
    29881 tgatcgggag cttcgcccca gcgtacagtt atcgctccct gagcgggcaa accggacata
    29941 tagagcgagc catcatcatc aataatgccg ctattagtct gtatgccgtc cagcgtcgct
    30001 atcgcgccaa aacgcagcgg tataccctgc tttgatgttt tcattaatac ccttgctccc
    30061 gcatgggcat tgaactccgc cagaaccaac gcccctttgg tgggaaccac gttgactact
    30121 gcattttcaa gatccacgtt acgttttaac gatgccgcat caagtgcgat tcgattttcc
    30181 cgataagaag tggcataggg gacaacggta tatccgcgcc agtcagtttt cagcccggtg
    30241 ctgttcgcca cacgagtatt ctctgcgcct ggtgctgcaa tcagcacgtt agtttccccc
    30301 agcgattgcc ccagggtaat gccctgtgaa tgggcaacta aactgcctga aagagcgtag
    30361 ttcagttgtt gttgactgcc gttatcgctg tagttgtaac ccactcgggc atctgcaaac
    30421 gcccctttgt agtccatgct ggcgctacca ttagccgttt tcccctcgct gttatatccc
    30481 tgttgcacgc tgtagctcag gttaccgtca tcgagtagtg tctccgttaa acccaggttt
    30541 tgtgtaatgt gcccttcgtt atcgatactg gtgttagagg tcatccaggc gtaatttttt
    30601 ttcctggtat agctatcatt acttctgggg agcaacagat tgagcggcag ggaaaaattt
    30661 agtgcaaaca cctgatcagc atcaggttga ccacgggact tactgtagtt ccaggaaacc
    30721 cccagcgaga gatctttaat ttgcgtgttg tagccaaact gtaatagccg atcctgttga
    30781 tcggtatgcc agtaagtttg ctggctacca cttaaataaa acgaaccgta ctcgcctaat
    30841 tgctgggaga tattgacctg cagtttgcca cgtttggtgt aaaacaaatt gtaataacgc
    30901 gaccacatcg gcgtatcttc atcatcaccg tcattaaatt cgtagccatc catatgttta
    30961 tacatggtgt cggaaagggt gtagaaaccc gacgtcgaat agcgatagcc catcaattga
    31021 aaattagtgc ccaattggtt gagcgttttg gcatacagaa aacgatatga ctgaccttta
    31081 tgttcgcttt ggtctgccag cgtactcttc gcctgggtcg catcgaacga aattgctccg
    31141 aaatcgccaa ggttaaatcc cagaccaaac atggcggcac ggtaatattc agcatattgt
    31201 ccaccaccat accatgtcgt tccccacggt ccgccccact gcaacgtggc ctgggcaaat
    31261 ttgctctcct gctgttcatt actattggtt ctgtatttcg ccagtgtcac cgcgtatttg
    31321 attcgcccct gacgctggag taatggcacg ctggaaaacg gtacgctgta gctattgacg
    31381 ctgccgtccg cttctttgat ttcaactaac aaatcaccgc tcgacgacgt ggaatagaga
    31441 tcactaattt caaaagcacc aggcgataca taagtttggt aaatggtgta accattttgc
    31501 ttaatcgtta tctgggcatt gcttttggcg ataccacgca ccacaggcgc aaaccctttc
    31561 aggctgtctg gcagcatgtt gtcatctgac gccagctgca caccacgaaa gctgacacta
    31621 tcgaaaaaat cgcctgcagt ataatcatca cctacggtca gttcgccttt tagtggaata
    31681 accgcccgct gcaaatagct gctgagattc ttccattctg cggtttggcc actactgcga
    31741 ctccatgttg aattgttgcg caatctccat gggcctaaat taacgccact gtttaaattc
    31801 agaaaatagc tgtcgccaga atcactgtct gcgctgctat gaatactgtt agccccgcta
    31861 aacgaatatc ccagtaatag cgcgttgatc ccttcatccc aacgttcagg ggggacataa
    31921 ccacgggcat tacttttgat ggcgatttgc ggtactgaaa tttccagacg gagtttattc
    31981 acattaaacg ttacgctggc atccggaata atctccgcca atggcacaca cgcttgtttg
    32041 tccagttgtt taaaagccgg aaacgcatca gtattaacac ccattgcatc caggctttca
    32101 gtcgtcaaac aggcggcaat ccccttctct ggcgttatct caacaaaatt gacgttgcgc
    32161 gtatctacaa ttgtctgatt cagaacaata tcgacacgat aaacccctgg cgttattttt
    32221 tgtccttttt cgaaacgaga caaatcggcc accattgagg gatcatcttt gaaaaaagca
    32281 ggatcgaacc aggtttcagc ttgtgcattg aacgctgccg acacaaacac caccagccca
    32341 ccaataaacg atggcactcc accagagctt aacaggctgt gttgtcgatg agttctatac
    32401 atctcgctct cttaactgaa gacggcttac gatttacagt tgagttgcgg cgtttccgcc
    32461 ccgtagtcat tgatggttcg atacgttacg ctgctatttt tggccccctt tggcagagaa
    32521 aattggtatt tatcaaaggg ggaaatcatt tgatttttaa ctacttttcc acctattttc
    32581 aggtctgtca gggtaatgaa ataaggtgtt gggttactga ccgtaaccat gtcagctgac
    32641 gaagtgcaac gcagtttcgc cggagcctcg cctaagggat acgctaaacc ttttggtcgc
    32701 cagaaaagtt tgatgacgct ttgtgtggca atcttcaggg tatttccctc ttcccgcttc
    32761 gttgttgaag ggactgcccg tacgctagtg aagaaaaggg tttctctgtc tttcgccaac
    32821 ggcgcaccaa tgtacataat gcgtaacaga ttctcattag ccggattcag cacaaatagc
    32881 ggtggtgtaa taataaaatc tgctgatttt ttattatctg gggtcatcac ccatgactga
    32941 ataagataag gaacatccgg actggtgtta cgtacaccca gtgaaatttg ctcttcacct
    33001 tctgaataaa caagacgtgt tgctgcaagt ccaatcccgc ctttacctgc cgcccatgcg
    33061 gatgagcagc tgagtaatat tgccgttaaa cttacggtca caattccctt tgttatgcaa
    33121 gttttcataa atagtcactc ttaaaaaata tgaatatccc ctccagaaca ctagaggggt
    33181 actaattaat aaaacaa
//
LOCUS       DAZFKM010000043        32775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFKM010000043 DAZFKM010000000
VERSION     DAZFKM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(53..214)
                     /gene="rsmS"
                     /locus_tag="ACHKAY_002123"
     CDS             complement(53..214)
                     /gene="rsmS"
                     /locus_tag="ACHKAY_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO5700802.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(228..755)
                     /gene="priC"
                     /locus_tag="ACHKAY_002124"
     CDS             complement(228..755)
                     /gene="priC"
                     /locus_tag="ACHKAY_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO5700803.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            825..1202
                     /gene="ybaN"
                     /locus_tag="ACHKAY_002125"
     CDS             825..1202
                     /gene="ybaN"
                     /locus_tag="ACHKAY_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO5700804.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1355..1906
                     /gene="apt"
                     /locus_tag="ACHKAY_002126"
     CDS             1355..1906
                     /gene="apt"
                     /locus_tag="ACHKAY_002126"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO5700805.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2035..3966
                     /gene="dnaX"
                     /locus_tag="ACHKAY_002127"
     CDS             2035..3966
                     /gene="dnaX"
                     /locus_tag="ACHKAY_002127"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO5700806.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3299..3363
                     /gene="dnaX"
                     /locus_tag="ACHKAY_002127"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4019..4348
                     /gene="ybaB"
                     /locus_tag="ACHKAY_002128"
     CDS             4019..4348
                     /gene="ybaB"
                     /locus_tag="ACHKAY_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO5700807.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4348..4953
                     /gene="recR"
                     /locus_tag="ACHKAY_002129"
     CDS             4348..4953
                     /gene="recR"
                     /locus_tag="ACHKAY_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO5700808.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5063..6937
                     /gene="htpG"
                     /locus_tag="ACHKAY_002130"
     CDS             5063..6937
                     /gene="htpG"
                     /locus_tag="ACHKAY_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO5700809.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7118..7762
                     /gene="adk"
                     /locus_tag="ACHKAY_002131"
     CDS             7118..7762
                     /gene="adk"
                     /locus_tag="ACHKAY_002131"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO5700810.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7998..8960
                     /gene="hemH"
                     /locus_tag="ACHKAY_002132"
     CDS             7998..8960
                     /gene="hemH"
                     /locus_tag="ACHKAY_002132"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO5700811.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8957..9916)
                     /gene="aes"
                     /locus_tag="ACHKAY_002133"
     CDS             complement(8957..9916)
                     /gene="aes"
                     /locus_tag="ACHKAY_002133"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO5700812.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10068..11372
                     /gene="gsk"
                     /locus_tag="ACHKAY_002134"
     CDS             10068..11372
                     /gene="gsk"
                     /locus_tag="ACHKAY_002134"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO5700813.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11505..13181)
                     /gene="ybaL"
                     /locus_tag="ACHKAY_002135"
     CDS             complement(11505..13181)
                     /gene="ybaL"
                     /locus_tag="ACHKAY_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO5700814.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13419..14639)
                     /gene="fsr"
                     /locus_tag="ACHKAY_002136"
     CDS             complement(13419..14639)
                     /gene="fsr"
                     /locus_tag="ACHKAY_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO5700815.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14857..16509
                     /gene="ushA"
                     /locus_tag="ACHKAY_002137"
     CDS             14857..16509
                     /gene="ushA"
                     /locus_tag="ACHKAY_002137"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO5700816.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16546..17025)
                     /gene="ybaK"
                     /locus_tag="ACHKAY_002138"
     CDS             complement(16546..17025)
                     /gene="ybaK"
                     /locus_tag="ACHKAY_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO5700817.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17229..18023)
                     /gene="ybaP"
                     /locus_tag="ACHKAY_002139"
     CDS             complement(17229..18023)
                     /gene="ybaP"
                     /locus_tag="ACHKAY_002139"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO5700818.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18161..18502
                     /gene="ybaQ"
                     /locus_tag="ACHKAY_002140"
     CDS             18161..18502
                     /gene="ybaQ"
                     /locus_tag="ACHKAY_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5700819.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18616..21120)
                     /gene="copA"
                     /locus_tag="ACHKAY_002141"
     CDS             complement(18616..21120)
                     /gene="copA"
                     /locus_tag="ACHKAY_002141"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO5700820.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21382..22314
                     /gene="glsA"
                     /locus_tag="ACHKAY_002142"
     CDS             21382..22314
                     /gene="glsA"
                     /locus_tag="ACHKAY_002142"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO5700821.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            22317..23609
                     /gene="ybaT"
                     /locus_tag="ACHKAY_002143"
     CDS             22317..23609
                     /gene="ybaT"
                     /locus_tag="ACHKAY_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5700822.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            23640..23737
                     /locus_tag="ACHKAY_002144"
                     /pseudo
     CDS             23640..23737
                     /locus_tag="ACHKAY_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23734..24141
                     /gene="cueR"
                     /locus_tag="ACHKAY_002145"
     CDS             23734..24141
                     /gene="cueR"
                     /locus_tag="ACHKAY_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO5700823.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24142..24600)
                     /gene="ybbJ"
                     /locus_tag="ACHKAY_002146"
     CDS             complement(24142..24600)
                     /gene="ybbJ"
                     /locus_tag="ACHKAY_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO5700824.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(24597..25514)
                     /gene="qmcA"
                     /locus_tag="ACHKAY_002147"
     CDS             complement(24597..25514)
                     /gene="qmcA"
                     /locus_tag="ACHKAY_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5700825.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            25660..26337
                     /gene="fetA"
                     /locus_tag="ACHKAY_002148"
     CDS             25660..26337
                     /gene="fetA"
                     /locus_tag="ACHKAY_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO5700826.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            26324..27103
                     /gene="fetB"
                     /locus_tag="ACHKAY_002149"
     CDS             26324..27103
                     /gene="fetB"
                     /locus_tag="ACHKAY_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO5700827.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(27166..28020)
                     /gene="cnoX"
                     /locus_tag="ACHKAY_002150"
     CDS             complement(27166..28020)
                     /gene="cnoX"
                     /locus_tag="ACHKAY_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO5700828.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(28081..28890)
                     /gene="ybbO"
                     /locus_tag="ACHKAY_002151"
     CDS             complement(28081..28890)
                     /gene="ybbO"
                     /locus_tag="ACHKAY_002151"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO5700829.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(28880..29503)
                     /gene="tesA"
                     /locus_tag="ACHKAY_002152"
     CDS             complement(28880..29503)
                     /gene="tesA"
                     /locus_tag="ACHKAY_002152"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO5700830.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            29474..30160
                     /gene="ybbA"
                     /locus_tag="ACHKAY_002153"
     CDS             29474..30160
                     /gene="ybbA"
                     /locus_tag="ACHKAY_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO5700831.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            30157..32571
                     /gene="ybbP"
                     /locus_tag="ACHKAY_002154"
     CDS             30157..32571
                     /gene="ybbP"
                     /locus_tag="ACHKAY_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO5700832.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7790 a         8639 c         8808 g         7538 t
ORIGIN      
        1 gttttgcgtt tgcccacaat cttaggggac atttagcggc cccatttatt tctcactttt
       61 ccgcctcatc atcgcgcgtt aatttctttt cataatcgcg ttttacaata tccagcgcgc
      121 gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacagcca
      181 gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgcta gcgggttaag
      241 cgcgctagcc tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca
      301 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg
      361 cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
      421 cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag
      481 gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc
      541 acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc
      601 tcatcgagac aagcctgcag tgttgtcgca cgggtttgaa aaaggtgtct gtcaaaacgg
      661 gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt agcgagctgt
      721 ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
      781 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
      841 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
      901 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
      961 ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
     1021 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
     1081 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
     1141 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
     1201 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
     1261 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt taaaacgcgc
     1321 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
     1381 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
     1441 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
     1501 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
     1561 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
     1621 caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcagct
     1681 ggagatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
     1741 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
     1801 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
     1861 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt
     1921 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
     1981 tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
     2041 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
     2101 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
     2161 ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
     2221 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
     2281 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
     2341 gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
     2401 tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
     2461 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
     2521 ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa
     2581 attcgccatc aacttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg
     2641 ttgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
     2701 caggcgattg ccagcggtga cggccaggtt tcaacccagg cagtcagtgc gatgctgggt
     2761 acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc caacggcgag
     2821 cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
     2881 gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
     2941 ggcaacgaca tggccgccat tgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
     3001 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
     3061 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccacgt
     3121 atgcctctgc ctgagccaga agtgccacgc cagtcctttg cgcccgtcgc gccaacggca
     3181 gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
     3241 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
     3301 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
     3361 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg
     3421 ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa ggctaccact
     3481 ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa
     3541 catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacgcg
     3601 tgggcggcac aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggccttaaat
     3661 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
     3721 ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca
     3781 acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg
     3841 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
     3901 aatattcaga ccctgcgtcg gttcttcgat gcggagctgg atgaagaaag tatccgcccc
     3961 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
     4021 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
     4081 gcagaaaatg caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct
     4141 ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct
     4201 gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc
     4261 acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct
     4321 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
     4381 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
     4441 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
     4501 tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt ctgtaacatc
     4561 tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag tccggcggac
     4621 atctacgcca ttgagcagac ggggcagttt tcaggccgtt attttgtgtt gatggggcac
     4681 ctgtcaccgc tggacggcat cggtccggat gatatcggcc ttgatcgtct ggaacagcgt
     4741 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa
     4801 gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc
     4861 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
     4921 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
     4981 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
     5041 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
     5101 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
     5161 tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa
     5221 cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa
     5281 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
     5341 tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca
     5401 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
     5461 ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt
     5521 ctgggaatcg gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg
     5581 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt
     5641 gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg
     5701 cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct
     5761 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
     5821 cgcccacgac tttaatgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga
     5881 gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga accgtgatca
     5941 taaacacggc ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt
     6001 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
     6061 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
     6121 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaca aataccagac
     6181 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca
     6241 ggaagcgatc gccaaactgc tgcgttttgc ttctacttat accgattctt ctgcgcagac
     6301 cgtgtctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat
     6361 cactgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
     6421 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
     6481 tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact
     6541 ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga
     6601 ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
     6661 cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggcgaaact
     6721 gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga
     6781 tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt
     6841 agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct
     6901 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg
     6961 tcatccggca tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg
     7021 tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt
     7081 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
     7141 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
     7201 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
     7261 agcaaaagac attatggatg ctggcaaatt ggtcaccgac gaactggtga tcgcgctggt
     7321 taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg
     7381 taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga
     7441 attcgacgta ccggacgaac tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc
     7501 gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt
     7561 taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct
     7621 ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc
     7681 gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgcgctga
     7741 tctggaaaaa atcctcggct aattcgaaag cgcgcacgga cagtcccctc gccccctcgg
     7801 ggagagggtt agggtgaggg gaacaggccc gcacaagcaa acttatcagc aatctcaggc
     7861 cggatattca ttcggccttt tacaaaaacc tcaatctgcg ctattccttt ttctgatttg
     7921 acctctcaca acaattagtt cttcttcctc acttttccgc tacaattatc aacaagttga
     7981 atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca aacctgggta
     8041 cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt ttaagcgaca
     8101 gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc gtgattttgc
     8161 cgctgcgctc gccgcgtgtg gcgaagatgt acgcctcggt ctggatggaa gacggctcgc
     8221 cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta ccggatacgc
     8281 ccgtagcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc gatgaactcc
     8341 tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc tcctgttcta
     8401 cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt agcattccgg
     8461 ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca ctggcgaaca
     8521 gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc tcttatcatg
     8581 gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc cgcacaacga
     8641 ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg acctttcagt
     8701 cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg aaaatgctcg
     8761 gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg gattgtctgg
     8821 agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc ggcgggaaaa
     8881 aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg atggctaatc
     8941 ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg cgaagagcct
     9001 cgtcggcggt tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga
     9061 gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat
     9121 ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg cgagtgagat
     9181 cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt aaatacgcct
     9241 cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc aacagacgac
     9301 gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc gcaattttac
     9361 cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc atggcacctg
     9421 cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc gcctgctggt
     9481 ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg
     9541 aaagggggta atcaatacca atcaccgtac attggctgta gcttgccagc aggcgcatga
     9601 tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg
     9661 tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca tattttgttg
     9721 gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat cggcgctcaa
     9781 gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac ggtaaatccg
     9841 gctgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga acaggtagtt
     9901 tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg aaccactgcc
     9961 atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc gctcccgtca
    10021 gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg aaatttcccg
    10081 gtaaacgtaa atccaaacat tacttccctg taaatgcacg cgatccgctg cttcagcaat
    10141 tccagccaga aaacgaaacc agcgccgcct gggtcgtagg tattgaccag acgctggtcg
    10201 atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc gccgggcatt
    10261 cactggtgat tgaggatgac gtagccgaag cgctttatca ggagctaaaa cagaaaaacc
    10321 tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcacaac tactcggtgc
    10381 tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa attggcagtt
    10441 atgcctatcg ttacctgtgt aacacttcca gccgtaccga tcttaactat ctacaaggcg
    10501 tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa cgtacctttg
    10561 ctatcagtcc aggccacatg aaccagctgc gggctgaaag cattccggaa gatgtgattg
    10621 ccggagcctc ggcactggtt ctcacctcat atctggtgcg ttgcaagccg ggtgaaccca
    10681 tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta ccggtggtgc
    10741 tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag caattcctca
    10801 aagaccacgt ttctatcctt gcgatgaacg aagatgaagc cgaagcgttg accggagaaa
    10861 gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg ctgtgcaccg
    10921 ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa cgtaaaaccc
    10981 agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt agccgcgcca
    11041 tgcgccacaa ggattgccag aatccgctgc gtgtatattc gcacattgcg ccgtacatgg
    11101 gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg gcagcgttgc
    11161 tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc agcaaacata
    11221 aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga
    11281 gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg gagcgtgaag
    11341 acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag gccagataag
    11401 gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg gcgcgtctta
    11461 tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc cgtcaccacc
    11521 tcacccgccg gtggcgtttc cagcagttcc agcatggtac gggcgatttc acgctcgccc
    11581 atcactacct gattcgcacc acgttcggtg atatacgcca cttcatcgtc ataatgggcg
    11641 cgggcaataa tctcaatatc cggatttttc gcgcgggcag atgccacaat ctcacccgct
    11701 tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg cgccagttgc
    11761 ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc tcgcagctca
    11821 tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc
    11881 tcccccagca ggctgcctac acgaccgtaa cccaccagta gcgcatggtt gcaaatatcc
    11941 actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg
    12001 gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat aatcgacagg
    12061 atcgcccctg ccagtactag gttttgtccg gcctgcggca gtaaattcaa tgccattccc
    12121 agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc gatggttaat
    12181 gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa atgcggctaa cgacttacca
    12241 aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag aattaacgga
    12301 tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc
    12361 gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac
    12421 gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat ccctaacgcc
    12481 agcgccagca ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct gcgtgccata
    12541 atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc gatcactttg
    12601 ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc
    12661 attcctgcca ctgcgggcag caacaccagc gtcagaacca ttaccaggtc ttccacaatc
    12721 aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc ttcaagtgcg
    12781 cgcagtaaca ccacggtact ggcggtggaa agacataaac cgaacacgat accggtcatt
    12841 aacgaccagc ccagcacggc ggagagcgcc atacccagca gcgtcgccac ggctatctgg
    12901 gcgatcgcgc cgggaatggc gatggacttt accgccatca aatccttgag cgaaaaatgc
    12961 aaaccgacgc cgaacatcag caaaataacg cccagttcag ccagttccgg cgcaagcttg
    13021 gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc
    13081 accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg
    13141 ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt
    13201 ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt atgacaattt
    13261 cattgatgat gttcatgaat aattattgaa ttttgcagaa aaatggaatt agctgcaaaa
    13321 aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg gcgactgtaa
    13381 aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg ccggttatca
    13441 ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt atagactaac
    13501 tcgatactgg tgtgatcggc gataagcccc agaactgccg ctcccagacc tcccatacca
    13561 aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag cagctcctga
    13621 gcgtaaacca gaatggcaga gaatgccgaa gcgaggataa atccaataat caccgttaaa
    13681 acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg
    13741 atagagcccc aaatcacata tttccgccca attttatccc ctacaggccc gccgatcacc
    13801 gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgagcatt ctggatagat
    13861 aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc catatagaaa
    13921 tatttcgaga aaatgaggat taacagaatg ctgaccgcca gtaccacttt gttgcgcggc
    13981 agtggattga taatcgtcgc tttgggtttt cctttattca ttcggtgctg tgccgagtac
    14041 caacggctga tttgcgccaa caccacgatc gccagcagtg ccgcaagcac aaaccaggca
    14101 acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc cagggaactg
    14161 ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc gccggaagcc
    14221 atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc gaccagcgcc
    14281 gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag accacttaag
    14341 gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg
    14401 accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc
    14461 ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat cagcgattgg
    14521 atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg
    14581 gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg gttgttcact cattgccata
    14641 ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga ttattacccg
    14701 actaacatac ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca gaatttctaa
    14761 tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc gtcatactat
    14821 ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg
    14881 gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc gtatgagcag
    14941 gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca tttttggcgc
    15001 aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa
    15061 gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc
    15121 gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc
    15181 tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt attacgccag
    15241 caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa aagtactggc
    15301 gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat tgccgtcatt
    15361 gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac tgatgtcgaa
    15421 tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag
    15481 ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga gcacggctct
    15541 aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc
    15601 gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa acaggtcgat
    15661 tacgtgccgg gtacgccatg caagccagat caacaaaacg gcatctggat tgtgcaggcg
    15721 catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg caaaatgaaa
    15781 atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg
    15841 aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat gatctcgctg
    15901 ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg cgaaaccaat
    15961 ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg
    16021 attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag cggaggcgga
    16081 attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtgctgaa agtgcagcca
    16141 ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga ttacctgacc
    16201 gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt
    16261 gtggcgaaag acggcagact gaacgacctt aaaatcaaag gcgaaccggt cgatccggcg
    16321 aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg atatccgcgc
    16381 cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg
    16441 tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc
    16501 tggcagtaat ccgaaagtgc cggatgtttg catccggcac aatgcttaat cgcggcgggc
    16561 gatatcagca aatttggcat cgaggatctt tgccagatcg cctgccgcca gttcgatatc
    16621 cagtccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat
    16681 aatcgttggc agacgttttt tctgccccag tgggctaatc cccccaacca ggtatcccgt
    16741 cgaacgctgc gcgaccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc
    16801 tactttttta agatccagtt gaccggcgac cggcgtaacg gccacggcaa ggtgtttcat
    16861 atcaccgttc actgccacca gcagcgtttt gtagacctga tccggattca aacctaattt
    16921 tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg tatggatttg
    16981 aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc
    17041 ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt
    17101 gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa
    17161 agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg
    17221 ccattttttt agcgcaacat ttgcggtaaa ttcccttctc catacaggtg tagtgcaccg
    17281 accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt
    17341 cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag cgttatttca
    17401 ttattttgcg gcggtgcatt cagccaccag ctcatcattt gttgcagcaa ccgtgcattg
    17461 gtgtgccagt gggtcagtgt atcgtccagc agcgccagtc ctttgtcagg gagctggagc
    17521 aacatggcaa tctggttttc agccccttcc agttcaatca cgggtttatg ttgttgcttc
    17581 gccgcctgca atagctggta atcgataccg tattctgccc gcagccccag tttttgtgcc
    17641 tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gggtagaaaa gagtgaggga
    17701 gaaatgccca tctcctggct aatgtgctgc agattttgga gttgctcctc gctaatgcgc
    17761 tcttccagcg cctcgcaggc aggcaaattg gcaaaaggcg tatcgctggt ggaaacatcc
    17821 gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggcagggga
    17881 gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccggga
    17941 agggtgatat cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt
    18001 tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg
    18061 ggagggatgg tgcaaatgga gttggggact tgtttaatgt gtttgtatga ttcagtatat
    18121 tcttgtatcg ctattcacag ggaagcaaca gttaaaaact atgaaacagg caacaagaaa
    18181 accgacgaca cccggagata ttcttctcta tgaatatctg gtaccgctcg atttgaaaat
    18241 caatgagtta gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa
    18301 caatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt
    18361 cgatttttgg ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg
    18421 cacccaggaa gaattgggac ggattgaaac agtggctgaa tacttggcac gccgtgaaga
    18481 gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc cggataaggc
    18541 gtttacgccg catccggcag tcatgcgtcg atgccaaatg tgccacccta aagcagcgca
    18601 tccgcaatga catacttatt ccttcggttt aaaccgcagc aaccggttgg cgttactcac
    18661 tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa gcagtgttcc
    18721 agtaaacggc cacaaaatac cggcggcgac cggaataccg atactgttgt agataaaggc
    18781 accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaatcgcga gcgcatccgc
    18841 aacgcccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg caacatcact
    18901 gccgcccccc atcgcaatac cgacatccgc ctgggccagc gctggcgcat cgttaatgcc
    18961 gtcgcccacc attgccacct gacgtccttc actttgcagg cgtttaatcg cttcggcttt
    19021 accgtccggc agcaccccgg cgatcacttc atcaatcccg gcttctttgg cgatcgcatt
    19081 ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt tatgcaggcg
    19141 ttgcagcgcc gccacgctat cactacgcaa tggatcgcgt actgccagca gggctaccgc
    19201 tttcccgtca accgccagca gcacaggcgt tgccccttgc gatgcctgag cagtaatctc
    19261 cgcttcgata gctttggtac caacctgttg ctcatttaac agcgcctgat tgcccagcaa
    19321 taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc ggaaaccgtt
    19381 gacctgcggc agctgcatat cacctgcttt atcgaggatc gcccgtgcca gcggatggct
    19441 ggaaccttgc tccagtgccg ccgccagacg caatgcctgc gcttcatcaa catcagcaaa
    19501 cgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt tatcgaacac
    19561 gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa
    19621 ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagcc ccagcgcaca
    19681 cggacaggcg ataatcagta ccgtggtggc aatcaccagg gtataaacaa tttgcggtgc
    19741 cggaccaaag aagtaccaga ttgccgcact gaccagcgca atcaccacca ccacaggtac
    19801 aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc tctgggcctg
    19861 gcgcaccatg cgaatgatcc gtgacagcgt ggtatggctg ccaaccgcac tggcacgaaa
    19921 cagcacgcta ccgtcctgta ccactgtccc ggcatggacg ctttcgcctt cgcctttttg
    19981 ctgcgggatt ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc cctgggtaat
    20041 ctcgccatct accggcacgc gatcgccggt cgtcaggcgc agcaacatac ctggctgcac
    20101 ttccgccaga ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg tcggtggggt
    20161 taaatcgagt aatttctcca gcgccttaga agaacgctga cgtgcgcgtg cttccagcat
    20221 atggccgaga ttgatcagac cgataatcat cgcgctggct tcgtaataaa gatgtcgcgc
    20281 ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc acgccacgcc
    20341 agtacccagc gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc ttttccatgc
    20401 actgcggtaa aaatggccgc cggcgaaaac catcaccgcc agggttatca ggccgataac
    20461 caaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccaatca tcccccagac
    20521 catcaccggg atacccaccg ccagtgcgac aattgcctgc cagcggaagc gcttcatcgt
    20581 agcaacggcg gtttcctgct ggcgctcacg gcgtttcgcg tcatcttcaa tcgcttccgc
    20641 gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac tgcccatcac
    20701 cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta cgctttgcag
    20761 cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca gcaactgctg
    20821 gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc
    20881 cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag atgcgtcata
    20941 acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc
    21001 ttcagtgata gacacatccg cttgttccac atccgggcgc tgttcgagac tttctttcac
    21061 gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgataa tttgtgacat
    21121 aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct tccagcaagg
    21181 ggaaggtcaa gaaattaata aaccaggcga gtaaaagtcc gtaaagatta aaaaattggc
    21241 tcaatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa gttgcgggtg
    21301 ctaagttaaa tcaagatgcc tgaaaatcgg caccggggtg aggaattacc tcccgcatct
    21361 ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc agtggatcag
    21421 gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgactacat tccctttctg
    21481 gcgaatgtac ctagtcaact ggcagcagtg gctattgtga cctgcgatgg caacatctat
    21541 cgcgcgggtg atagtgatta ccgctttgcg ctggaatcca tctcgaaagt ctgtacgtta
    21601 gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaaattgg cgctgacccg
    21661 accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa accactgtcg
    21721 ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga aaatattgaa
    21781 caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagca ggtagcgctc
    21841 tctgatgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc cattgcctgg
    21901 ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga cgtgtacacc
    21961 cgtcagtgct ccacgctcat caatactgtt gaactggcaa cgcttggcgc gacgctggcg
    22021 gcgggtggtg tgaatccgct gacgcataaa cgcgttcttc aggccgacaa cgtgccgtac
    22081 attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc
    22141 gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt ccctggcgtg
    22201 atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaacagtgt tcgcggtcaa
    22261 aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg ctgatcatga
    22321 tgaacacgga aggtaataac ggtaacaaac ctctcggtct atggaacgtc gtttccatcg
    22381 gcattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct gcattgctaa
    22441 tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg ttttccggtt
    22501 atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggcattatc gacttctttc
    22561 gtcgcggatt aggcaacggc gtcttttcgc tggcgctctc gttactgtac ctgttgacgc
    22621 tggcggtgag catcgccatg gtcgcccgtg cttttggcgc ttatgccgtg cagtttttgc
    22681 atgaaggcag ccaggaggag caccttattt tgctctacgc gttggggatc attgcggtga
    22741 tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg atcctcgtcg
    22801 gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg ctgcaaccgg
    22861 cgcatatttc cgtctctgcg ccccccagct ccggtgcgtt cttctcctgt atcgggataa
    22921 ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa gtgaaagatc
    22981 cgcaggtcat tatgccgcgg gcgtttctgg tggcgattgg cgttaccacg ttgctttata
    23041 tctcgctggc actggttttg cttagcgatg tatcggcatt agagttagaa aaatatgccg
    23101 ataccgccgt agcgcaggct gcttctccgc tgctcggaca tgtgggttat gtgatcgtcg
    23161 tcatcggcgc tttactggcg acggcttcag ccattaacgc gaacctgttc gccgtgttta
    23221 acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat aaatccctgt
    23281 ggcggcagag tacctggggc aacatcattg tcgtggtgtt gattatgctg atgacggcgg
    23341 cactaaattt aggctcactc gccagcgttg ccagcgccac ctttttgatt tgctatctgg
    23401 cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg ccgattctta
    23461 tcgttggtac gttggtgatg ttgttggtga tcgttggctt tatctacagt ctgtggtccc
    23521 agggtagccg tgcgttgata tggattattg gctcactctt actcagcctt attgtggcaa
    23581 tggtcatgaa gcgcaataaa accgtataac atctctctgt gcgcagtact tcctgtatta
    23641 ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc
    23701 ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca tcagcgatgt agcaaaaatt
    23761 accggcctga ccagcaaagc cattcgcttc tatgaagaga aggggctggt gacgccgccg
    23821 atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga actgacctta
    23881 ctgcgccagg cacggcaggt gggctttaac ctggaagaga gcggcgagct ggtgaatctg
    23941 tttaacgacc cgcagcggca cagcgccgac gtcaaacggc gcacgctgga gaaggtggcg
    24001 gagatcgaac gacacattga ggagctgcaa tccatgcgcg accagctgct ggcactggcg
    24061 aatgcctgcc ctggcgatga cagcgccgac tgcccgatta tcgaaaatct ctccggctgc
    24121 tgtcatcatc gggcagggtg attaagacga gacggctcgg atatgtaggg ttatcccttc
    24181 tatcgcaatg acttcaacat gcgtacctgc accgagatcc tcgctggcgc tgacaggcca
    24241 tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc agcggagatt ccagcacaaa
    24301 acgtcggcca atcagctgct gcccgcgctg gtttaaatga ctatcgctgt gcttttgttc
    24361 gcgcacccgc cgcgacaacc atttccacca cagccaggcg gcgagcagcg tcaggatggc
    24421 aaacattacc ccttgccact cccaacccag cggcaccagc cagaccacca ggccggtaat
    24481 caccgctgcc acgccgctcc acaacaaata accatttccg cccagcatct cggctgccag
    24541 cagcaaaccg ccgagactga gccagaaaat atgtggatga acgaccatta actccatcat
    24601 ggctgagtcc gtttgttggc gctgtctttc accagctcgg caatcccggc aatcgacccc
    24661 atcaggctgc tggcctctaa tggcatcatc actactttgc tgttactgga ggaaccgatc
    24721 tgctgtaacg cttcggtgta tttctgcgct acgaagtagt tcaccgcctg aatatcaccg
    24781 gaggcgatag cttcagacac cattttggtg gcgcgggctt ctgcttcggc ggaacgttca
    24841 cgcgcttcag cctgtaaaaa cgccgactga cgttcgcctt ccgctttcag gatttgcgac
    24901 tgtttttcac cttcggcttt gaggatttcc gcctgacgga tcccttccgc ttcaagaatg
    24961 taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca ttgaagagat aagctctgcc
    25021 ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa tcccccacgg gttggtggct
    25081 tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc gctgagagag catttcgtca
    25141 agttccattg aacccaacac ggtacggatg ttagtcatgg tcaggttgat gatcgccagc
    25201 tccagattgc tgacttcata agccgcgcgc ggcgcgtcaa tcacctgaat aaaacagact
    25261 gcgtcgatgg taacgttggc gttatctttc gagataactt cctgggaagg gatatcgagc
    25321 acttgctcca tcatattgat cttgcgacca atgcgatcca taaacggcac caccagactg
    25381 agccccggct gtaacgtttt ggtatagcga ccaaaacgtt ctaccgtcca ttgatagccc
    25441 tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg
    25501 ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca aaattgctgc
    25561 ttgattaaac aaattatacc tgattactga aagagagttc ccccttattc ctgcgaagga
    25621 taaactgttt ttagtaaaaa tcagaaaaag ggaacagtga tgcaggaaaa tagtccttta
    25681 cttcagctac aaaacgtagg atatctggcg ggtgatgcga agattcttaa taacatcaat
    25741 ttttcgctgc gtgctggcga atttaagtta attaccggtc cttccggttg tggcaaaagt
    25801 acgctgctaa aaatagttgc ttcattgatc agcccaacca acggaacgtt actgtttgaa
    25861 ggtgaggatg tcagcacact aaagccagaa atctaccgcc agcaagtctc ttactgcgcc
    25921 cagacgccga cgctgtttgg cgatacggta tacgataatc tgatctttcc ctggcagatc
    25981 cgtaaccagc agcctgaccc ggccattttt ctcgattttc tcgaacgctt cgctttgccg
    26041 gacagcattt tgacgaaaaa tatcgccgag ctatctggtg gtgaaaaaca acgcatctca
    26101 ctgattcgta acctgcaatt tatgccgaag gttttattgc tggatgaaat caccagtgcg
    26161 ctggatgaaa gtaataaaca taacgttaat gagatgatcc atcgttatgt gcgcgagcaa
    26221 aatattgccg tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc ggataaagtg
    26281 attacactgc aaccgcatgc cggagaaatg caggaagcac gctatgaact cgcataatat
    26341 tactaacgaa tcattggcac tggcattaat gctggtggtg gtggcaatct taattagcca
    26401 taaagaaaaa ctggcgctgg agaaagatat tttctggagc gtcgggcgag cgataattca
    26461 gctgattatt gtcggctatg tgctgaagta tattttcagc gtggatgatg ccagcctgac
    26521 attattgatg gtgttattta tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa
    26581 atatattgct aaagctttta tctcatcgtt tattgccatt acggtcgggg cgggaattac
    26641 cctggcggtg ctgattctct ctgggtcgat tgaatttatc ccgatgcagg tgatccctat
    26701 cgccgggatg attgccggta acgccatggt agcggtgggg ttgtgttaca acaatttagg
    26761 gcaacgggtc attagcgaac agcaacagat tcaggagaaa ctgagtcttg gtgcgacgcc
    26821 gaagcaggct tcagcgatat tgattcgcga cagtattcgc gcggctttaa ttccgacggt
    26881 cgattcagca aaaacggttg gcttagtgag tttacctgga atgatgtccg ggctgatatt
    26941 tgccgggatt gatccggtga aggcgattaa atatcagatt atggtgacct ttatgctgct
    27001 ctcaaccgcc agcttgtcga ccattattgc ctgctattta acctatcgta agttttataa
    27061 ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa tgatgcgatg ccggatgcgc
    27121 agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg gaatatcaat acaacaatgc
    27181 atacagctgg cggcgatact tcgacgccag tgcatcaccc gtacccagcg cagcgaggat
    27241 ctcctgaaac gttttacgcg tctgaccgtc tgcggcggtg agatctttac gcagatgccc
    27301 gaacagcaac tccagcgcct cttcattgcg cccaacctgg tgcagttgca gagccagttg
    27361 cgtcgccagt gcggcatctt ctggattctc cgccacctgc tgttgcaact gttgaatttc
    27421 cggcgtatca gccgcctgct tcagcagttc gatttgcgcc accagcccct ggtagcgggt
    27481 gtcctgatcc tgcaacggaa tggttttcag caccgtttcc gcatcttcag aacggttcag
    27541 cgcaatcagc gtttctgcca gcagcaggcc gatctccccg ttctgattcg acaactgcca
    27601 ggcgtctttc agcaatggca gggcatcggt gtaattgcct tcctgcatca gttgcatcgc
    27661 ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc
    27721 ctcttccggt tgcggcccct ggaagccatc taccggttgc ccgttctgga acagatacac
    27781 ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt cgcagtccag
    27841 cttcgccaga ataaattgcc cgttgtactg cgccgcgagg ctttccagga tcggggttaa
    27901 ctgcaaacag tgctggctac gttcagacca aaaatagaac agcaccggag tggtcatcga
    27961 ctgttcaaga acctgttgca ggttagattc gttaatgttg acaatatttt ctacggacat
    28021 ggagtcgctc tctgttgtcg atattttctt tgacatgggg gcttaagcgc gcgcttcaac
    28081 tcacccctgc aatattttgt ccatcacgcg ccccggcagc aggcgcttga gcaccattac
    28141 tgcccaggtc accagcgtta ccggatagcg catcttcggc ttctcgctaa taaaagcatg
    28201 gcgtactttg tccaccaccg cttccggtcc caacgtaaag cgggcggcga tgccgggatt
    28261 ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg
    28321 accgggttcg atcaggctga ctttaattcc gctgtggcgc agctccatgc gcagtgcgtc
    28381 tgaccacgcc tccagcgcat atttactggc cgcgtaagcg ccacgacccg gcgtggagat
    28441 taatcccatc accgatgatg tcatcacaat acgcccttca ccgtgcggta acatcgcggg
    28501 taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat
    28561 ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg aatccggcat tgttaaagat
    28621 cccatacaga caattatcgg tcagggcgat cacctcgtct gctgcgcgat caacactttc
    28681 tggtgaatcc agatcgatca acacgccggt aaatcccatg ttgttcatac gctcaacatc
    28741 atccggtttt cggcaacctg ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct
    28801 ttccaggcca attccactgg aacatccggt aattaagacc gatttttgca taactttacc
    28861 tgtcaggatc tccgttgctt tatgagtcat gatttactaa aggctgcaac tgcttcgcca
    28921 tccagtcggc aataaacggc tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc
    28981 attgtggctt gaggtagacc tcttccataa aaaagggcag cagcggaaca tcaaactctt
    29041 tggcgagttt ggggtaaatg gcgctaaagg cttcattata acggcgacca tagtttgcag
    29101 gcagacgtat ttgcattaac aatggttcag cgttggcggc tttgacatcc tgcaaaatct
    29161 ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg caaaccgtca ttgccgccca
    29221 gttcaaccag cacccagcgc ggctgatgct gtttcagcag agccggaagg cgcgccagtc
    29281 cttgttgcga ggtgtcgccg ctgatgctgg cattaactac cgacgtttta ctctgccact
    29341 tatcattcaa caaggcaggc caggccgcgc tggcagacat tcgatacccg gcgctcaggc
    29401 tatcacccag aatcaataac gtgtccgctg cggcggcacg gaaggttaac aggaccagga
    29461 acaggaaggg caaatgccag cggaaaacat tgttgaagtt catcatctta agaagtccgt
    29521 cggtcagggg gagcatgaac tctccatcct caccggagtt gagctggttg tcaaacgtgg
    29581 cgagaccatc gcactggtgg gcgaatcggg atcgggtaag tcaaccttgc tggcgatcct
    29641 cgccgggctt gatgacggca gcagtggcga agtgagtctg gtgggacaac cgctacataa
    29701 tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac gtcggctttg tttttcagtc
    29761 atttatgtta attcctaccc ttaacgcgct ggaaaatgtc gagcttccgg ctctgctgcg
    29821 cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg ttgctcgaac agttagggct
    29881 gggtaaacgt ctggatcatc ttccggcaca gctttccggc ggtgaacagc aacgagtggc
    29941 gctggcacga gcctttaatg gtcgacctga tgtgctgttt gccgacgaac ccaccggcaa
    30001 ccttgaccgc cagacgggcg ataaaattgc cgacctgctg ttttccctca accgtgaaca
    30061 tggcaccacg ttgattatgg tgactcacga cctgcaactg gcggcacgct gcgaccgctg
    30121 cttacggctg gtgaacgggc agttgcagga ggaagcatga ttgcacgttg gttctggcgc
    30181 gaatggcgct cgccgtcgct attaattgtc tggctggcgc taagcctggc ggtggcctgc
    30241 gtgctggcgc tgggcaatat cagcgatcgc atggagaagg gcttaagcca gcaaagccgt
    30301 gagtttatgg cgggcgatcg ggcgttgcgc agttcgcgcg aagtgccgca agcatggctg
    30361 gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc caccatgaca
    30421 tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg tggatgatat ctacccgatg
    30481 tatggcgatc tgcaaactaa tccccctggc ctgaaaccgc aggcgggcag cgtattgctg
    30541 gccccacgcc tgatggcact gcttaacctg aaaacgggcg acaccattga cgtgggcgat
    30601 gccaccttgc ggattgccgg agaagtgatt caggaaccgg attccggttt taaccccttc
    30661 cagatggctc cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg agccgtgcag
    30721 ccggggagtc gggtcacctg gcgttataaa ttcggcggca acgagaacca gctcgacggc
    30781 tatgagaaat ggttgttacc tcagcttaaa cccgaacaac gctggtacgg tctggaacag
    30841 gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac agttcctgct gctttcggcg
    30901 cttctgacct tgctgctggc agtggcagcg gtggcggtag cgatgaatca ttactgtcgc
    30961 agtcgctacg atctggtggc gatcctcaaa acgctggggg cagggcgagc gcaactgcgt
    31021 aagctaatcg tcggtcagtg gttgatggtg ctggcgcttt cagccgttac cggtggggcg
    31081 ataggcctgt tgttcgaaaa cgtgttgatg gtgctgctca agcccgttct gcctgctgca
    31141 ctaccgccag ccagcctctg gccgtggctg tgggcgctgg gtaccatgac ggtcatctcg
    31201 ctgctggtgg ggctacgacc gtaccgcctg ttgctggcaa cgcagccttt acgcgtatta
    31261 cgtaatgatg tggtagcgaa cgtctggccg ctgaagtttt atctgccgat tgtcagtgtg
    31321 gtggttgtgc tgctgctcgc cggattaatg ggtggcagca tgctgctttg ggcggtgctg
    31381 gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg gctggatgct gctgaatgta
    31441 cttcgccgca tgacgctgaa atcgctacct ctgcgcctgg cggttagccg cctgttacgt
    31501 cagccgtggt caacattaag ccagctttcg gcattttcgc tctcctttat gctgctggcg
    31561 ctgttgctgg tattgcgtgg cgatctgctt gaccgctggc agcagcagct acctccagaa
    31621 agcccgaact actttttaat taacattgcc acagaacagg tagaaccgct aaaagcgttc
    31681 ctcgcggaac atcagatagt cccggaatca ttttatccgg tagtgcgggc gcggctgacg
    31741 gcgattaacg ataagccgac agaaggtaat gaagatgagg cgcttaaccg cgaactcaat
    31801 cttacctggc aaaatacgcg acccgatcat aatccgattg tcgccggtaa ctggccgcca
    31861 aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt tgccctcggt
    31921 gataccgtga cttttatggg cgatacccag gagttccgcg ctaaagtgac cagcctgcgc
    31981 aaagtggact gggaaagtct gcggcctaat ttctatttta ttttccctga aggggcatta
    32041 gacgggcaac cgcagagctg gcttaccagt ttccgctggg agaatggcaa cggcatgttg
    32101 acacaactca accgccagtt cccgaccatt agcctgttag atattggcgc gattttaaaa
    32161 caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg aggtgatggt ggtgctggtc
    32221 accgcctgcg gtatgttgct gttgctggca caggtgcagg tgggaatgcg ccagcgtcat
    32281 caggagctgg tggtatggcg cacactcggt gcggggaaaa aactgctgcg cacgacattg
    32341 tggtgtgagt tcgccatgct tgggtttgtt tccggcctgg tggccgcaat tggtgcggaa
    32401 acggcactgg cagtgttgca ggcgaaagtg tttgatttcc cgtgggagcc agactggcga
    32461 ttgtggattg ttctgccgtg cagcggtgcg ctgctgctgt cgcttttcgg cggctggctg
    32521 ggtgcgcgac tggttaaggg taaggcgctg ttcaggcagt ttgcggggtg atgagagtaa
    32581 ataaggggcg ttctggttga atggaacgcc ttgtttaaat taattctaca atatgtttca
    32641 ttgatgatgt tattgaattg gtgatttcct atctttctat tgcgatatta atctgaatcg
    32701 gtggagttta ataattttaa atatagccct acaaaaataa ctcagactaa taaaatcata
    32761 aatcatatgc gttga
//
LOCUS       DAZFKM010000044        32630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFKM010000044 DAZFKM010000000
VERSION     DAZFKM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..177
                     /locus_tag="ACHKAY_002155"
     CDS             <1..177
                     /locus_tag="ACHKAY_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5700833.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHKAY_002156"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHKAY_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHKAY_002157"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHKAY_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO5700834.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHKAY_002158"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHKAY_002158"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5700835.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHKAY_002159"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHKAY_002159"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO5700836.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHKAY_002160"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHKAY_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHKAY_002161"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHKAY_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..5368
                     /locus_tag="ACHKAY_002162"
     CDS             4301..5368
                     /locus_tag="ACHKAY_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5700837.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHKAY_002163"
     CDS             complement(5615..6277)
                     /gene="ytfE"
                     /locus_tag="ACHKAY_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO5700838.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHKAY_002164"
     CDS             complement(6385..7350)
                     /gene="ytfF"
                     /locus_tag="ACHKAY_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5700839.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHKAY_002165"
     CDS             complement(7458..8318)
                     /gene="qorB"
                     /locus_tag="ACHKAY_002165"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5700840.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHKAY_002166"
     CDS             8407..8787
                     /gene="ytfH"
                     /locus_tag="ACHKAY_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO5700841.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHKAY_002167"
     CDS             complement(8916..10859)
                     /gene="cpdB"
                     /locus_tag="ACHKAY_002167"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO5700842.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHKAY_002168"
     CDS             11049..11789
                     /gene="cysQ"
                     /locus_tag="ACHKAY_002168"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO5700843.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHKAY_002169"
     CDS             complement(11779..12336)
                     /gene="ytfJ"
                     /locus_tag="ACHKAY_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5700844.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHKAY_002170"
     CDS             12661..12867
                     /gene="ytfK"
                     /locus_tag="ACHKAY_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO5700845.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHKAY_002171"
     CDS             complement(12929..14272)
                     /gene="paeA"
                     /locus_tag="ACHKAY_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO5700846.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHKAY_002172"
     CDS             complement(14595..15233)
                     /gene="msrA"
                     /locus_tag="ACHKAY_002172"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO5700847.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15439..17172
                     /gene="tamA"
                     /locus_tag="ACHKAY_002173"
     CDS             15439..17172
                     /gene="tamA"
                     /locus_tag="ACHKAY_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO5700848.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17169..20948
                     /gene="tamB"
                     /locus_tag="ACHKAY_002174"
     CDS             17169..20948
                     /gene="tamB"
                     /locus_tag="ACHKAY_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO5700849.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHKAY_002175"
     CDS             20951..21292
                     /gene="ytfP"
                     /locus_tag="ACHKAY_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO5700850.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21504..21755
                     /gene="chpS"
                     /locus_tag="ACHKAY_002176"
     CDS             21504..21755
                     /gene="chpS"
                     /locus_tag="ACHKAY_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO5700851.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21749..22099
                     /gene="chpB"
                     /locus_tag="ACHKAY_002177"
     CDS             21749..22099
                     /gene="chpB"
                     /locus_tag="ACHKAY_002177"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO5700852.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHKAY_002178"
     CDS             complement(22179..22709)
                     /gene="ppa"
                     /locus_tag="ACHKAY_002178"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO5700853.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHKAY_002179"
     CDS             23019..23975
                     /gene="ytfQ"
                     /locus_tag="ACHKAY_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO5700854.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHKAY_002180"
     CDS             24115..25617
                     /gene="ytfR"
                     /locus_tag="ACHKAY_002180"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO5700855.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHKAY_002181"
     CDS             25631..26653
                     /gene="ytfT"
                     /locus_tag="ACHKAY_002181"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO5700856.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHKAY_002182"
     CDS             26640..27635
                     /gene="yjfF"
                     /locus_tag="ACHKAY_002182"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO5700857.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHKAY_002183"
     CDS             complement(27668..28666)
                     /gene="fbp"
                     /locus_tag="ACHKAY_002183"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO5700858.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28842..30215
                     /gene="mpl"
                     /locus_tag="ACHKAY_002184"
     CDS             28842..30215
                     /gene="mpl"
                     /locus_tag="ACHKAY_002184"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO5700859.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHKAY_002185"
     CDS             complement(30371..30922)
                     /gene="yjgA"
                     /locus_tag="ACHKAY_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO5700860.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHKAY_002186"
     CDS             31016..32368
                     /gene="pmbA"
                     /locus_tag="ACHKAY_002186"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO5700861.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32551..>32630
                     /locus_tag="ACHKAY_002187"
     CDS             32551..>32630
                     /locus_tag="ACHKAY_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5700862.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         7729 a         8134 c         8909 g         7858 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg acactggagc agggagagca gatgccagaa tggacggaca ggaaaatgga
     5281 gggggagtat aaaagaggcg agaaattcca tgtgctgtgg tcgctgatga accgccatga
     5341 tggcgggtcg gtgtgggtgg aagcgtagcg gtagctggat gtacacgtaa aaaccaggga
     5401 atgggtgatt ttattctggc ttatacagga gcgatgaata agtaattata ttaatgaaaa
     5461 aaataagtta gtaaccgttg aaggaactta ttattggctg aataatgaat tttcccagtt
     5521 cagtagatac aaaagattgt cccggagtgg tgaataactc aaagcatttg aaaacgcacc
     5581 tgattgtgta aaccaggtgc gttgatcgaa caaattactc acccgccagc gcgcgtggga
     5641 acagtacatt gttttccagg ctgatgtgat ccatcaggtc atcaatcagt tcattaatac
     5701 cgttatacat cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg
     5761 tgtgtttaat cacttccagc agttcgcccg cttcatcgtg ctcgctttcc atcacgctga
     5821 ttggtcccat tgcctggctg cccatgcctt gtttgatcat cgggaagagg atctgctctt
     5881 ctttcatcat gtggctggaa agctcttcat gcagcatagt caggtatttt gtcagccctt
     5941 ttggcacgct cggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct
     6001 ccggcagctg ctcgcggtga cgatcgtggt agcgcacgat gatatggtca atgatttctg
     6061 ccagcggggc gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcag
     6121 cttcaatgac ctcaacatcc agttctttac gtgccgccgc gcgcgccagc gtctgcttac
     6181 cgccacagca gtaatccata tcatatttac gaaacagagc cgaagcgcga gggatagaga
     6241 gcgccagttc acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa
     6301 gataagatgt attttaaatg catctttaag gcaaaagcta taacccttac gtagcagagg
     6361 cttatttaac tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc
     6421 tggttttgct ctgaccgcaa tgaccacgcc aatcactaac agcgcgatac cgctcagcgt
     6481 cattagcggc ggcatttgct ggcggagtaa aaaggtgtac agcaaacctg ccagcgtttc
     6541 gaaaacaatc agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca
     6601 gagtgcgcca acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag
     6661 cggacggggg ccaaaaggta aggagaagtc cggcgtttgt atattcagcc agtaacaggc
     6721 gacgagatag ccgatgagag aaaccggcag cgtgaccagc gcctgcgccg tcgcccacat
     6781 catcggatgt ttgtcgggat tttcccgcag ccagcgggcg ttgcgcaggg cataccatgc
     6841 ccagcagacc acggaaacta acgctagcac gatgccagag gtataacgtg cccagtcaaa
     6901 atcggggagt ccgtggttta actcagcaat attcacactc gccaggccga tgccaataca
     6961 aatcagtgcc ggggcgagtt ttccccacgc gagtttgccg tcgcgctggc tataaagcag
     7021 attggcaaag acaggaatga ccaccggcag ggtgccgata atcatcgtgg aaacaggcgc
     7081 gccagtacgt tgaatggcac tggcaaggca gaagtaataa atgaggttgc ccatcatagt
     7141 gagcatcaag gcggtaagcc agtcccgacg cgccaactga cgcagacgca cgcgtcccag
     7201 ccaggcaatg ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat
     7261 cgccgggtat tccggtacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc
     7321 taacagggcg tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa
     7381 gttcaggaag cagacatgat tgctcccggc atcccgtcat tattagagag gatgcgggag
     7441 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     7501 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     7561 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     7621 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ctgtttgccg
     7681 ctctgtttgg tcagctctgc cgctaactgt gtcaatgtcc aggcactatc gcccgccagt
     7741 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     7801 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     7861 tccagtgctg ccggggcgct ggcgaggtag ttttcgctgt accagccgtt gcgcagcagg
     7921 gtgtaaacga tgccagaatc agccaacatt ttctccgtct cgatgtgctc atcggcgagg
     7981 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacaccagcc
     8041 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     8101 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     8161 tagtcagcct gacgcacggt aatgccttgt gctgccagag cctgggcttt tgccggatta
     8221 cgaacgatag ccactatttg gctgacagga accgttttct tcaaggattc aataacatag
     8281 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     8341 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgcaca aaaaggtaag
     8401 tatgaaatga gtcaggttag cctgtcgcaa caactgaaag agggtaatct ctttgcggaa
     8461 cagtgcccgt cgcgcgaggt gttgaaacac gtcaccagcc gttggggggt gttgattctg
     8521 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat cggcggggtg
     8581 agcgaaaaga tgcttgcgca gtcgttacag gcgttagaac aggatggttt tcttaaccgt
     8641 atcgcgtatc cggtggtgcc gccgcatgtg gaatatagcc tcacgccgct gggcgagcag
     8701 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     8761 gcggtgcggg atgaacgtgc ggcataactg ccggatgcgc tgagcttatc tggtctacgg
     8821 tgcatggctt gtagggcgga taagatgcgc cagcatcgca tccgccaata aatgccggat
     8881 gcggttgatg atttataggc cgaaaaagaa gtgttttact tactcaaatc cacctgataa
     8941 atcgcaaacc cgatatcatc ggtcgcgact ttattcatcg gatattgccc tttctcttta
     9001 ataaacgctg cggctttatc tgacggagaa gtttcgaaac ggatatccag tttcttatcg
     9061 ccagctatcg gtgctaaacg ccagttgtta tctgccgccg ggtgaatttc ccccgcacgt
     9121 ttcgactcat cagcaatcca cgctgccagc accgagcggt tctcatccgg tgaagcaaaa
     9181 gcgatatggc tgtcgcccgt accggcaaat ttgccgccgt aagcgcgata gttattggtg
     9241 gcaacgagga acatggcgtt cggatcaatc ggcttgccat taaaggtcag gttcttaatc
     9301 ctttccgcat tggcattaat catctggcac tcgccgtcat aacgggcagg ctgggtaaca
     9361 tcaatctgat aattcacacc atcaatcaca tcaaagttat aggtgcggaa accatcccag
     9421 ttgatgagtg attgcggttt cgtgctgtta ggatcaatct ggttaaactg tccggcggag
     9481 cactccagcc actctttcac ctctttaccg ctggctttca ccacaatcag cgtattgggg
     9541 tagagataaa gatcggcggc attacggaag gtcaactggc ctttttccac ctccacatag
     9601 cttgccgggt catttttgcg accaccaact ttaaacggtg cggcagctga aagcaccggc
     9661 agttttgcca gatccggatc gccctgaatg taatgttcga catacgcttt ttgcgcgttg
     9721 ttcactactt gcacggtcgg atcgtcctgc accagcgcca gatagctata catattgtcg
     9781 gcagatttac cgattggctt gctgacgaac tggcgtgtgg catcgtgatc ggctttgagt
     9841 gtttctacca gcttgctgtc ttccgccgcg agggattttt tattggcgat gtcgtaaatc
     9901 ggccgagctt ccgctttcgc ctgcgtcacc tgccatttac cgctgtcatt actgagttgt
     9961 aagtcgacca ccccaagatg atcgccccac atgcccggca ttaccgccgg aacaccattc
    10021 agcgtgcctt tggtgatatc agccccttcg atatcagcaa aatctttacc cgggaaaacg
    10081 gcgtgagcat gaccaaacat aatggcgtta acgcccggaa tttcactgag gtaataaact
    10141 gagttttccg ccattacttt atacggatcg gcagacagcc cggaatgcgc cagaacgaca
    10201 acgacatcgg cacctttctc gcgcatttca ggcacgtatt tgcgcacggt ttcggtaata
    10261 tcattcaccg tcacttttcc ggataaatta gctttatccc agcccatgat ttgcggcggc
    10321 acgacgccaa tatagccaat cttcagcgtc tgtttttttc cgtctttatc gaccacttcg
    10381 gtatctttaa ttaaatacgg tgtaaacatt ggctgtttgg ttctggcgtc aatgacgttg
    10441 gcatttacat aagggaattt cgctcccgcc agcgcatttt tcaggtaatc cagaccgtag
    10501 ttaaattcat gattgccgag tgtaccgacc gtataatcca gcgtattcag cgccttataa
    10561 accggatgaa catcacctgc ttttaatcct ttcgccgaca tgtaatcggc cagcggactc
    10621 ccctgaatca aatcgccgtt atcgaccagt acgctgtttt tcacttcatt gcgggcatcg
    10681 ttaatcaggc ttgccgtacg taccagtccg aatttttccg tggcggtgtc tttgtaataa
    10741 tcgaaatcca tcatgttgct atgcagatca gtggtttcca tgatacgcag atcgaccgtc
    10801 gctgcattca cactggcggc aatcagcgtg gccaggagcg ttgcgctaaa cttaatcatc
    10861 agggacatcc ttttatcatc gggaatacga aagaaaaggg agaataaacg tcttacttat
    10921 agaacagtga agaatgccac aattttacgc tttgaaaatg atgacactat cacagttggc
    10981 gcattcatta acgatagggt ataagtaaaa caataagtta acaccgctca cagagacgag
    11041 gtggagaaat gttagatcaa gtatgccagc ttgcacggaa tgcaggcgat gccattatgc
    11101 aggtctacga cgggacgaaa ccgatggacg tcgtcagcaa agcggacaat tctccggtaa
    11161 cggcagcgga tattgccgct cacaccgtta tcatggacgg tttacgtacg ctgacaccgg
    11221 atattccggt cctttctgaa gaagatcctc ccggttggga agtccgtcag cactggcagc
    11281 gttactggct ggtagacccg ctggatggta ctaaagagtt tattaaacgc aatggcgaat
    11341 tcaccgttaa cattgcgctc attgaccatg gcaaaccgat tttaggtgtg gtgtatgcgc
    11401 cggtaatgaa cgtaatgtac agcgcggcag aaggcaaggc ctggaaagaa gagtgcggtg
    11461 tgcgcaagca gattcaggtc cgcgatgcgc gcccgccgct ggtggttatc agccgttccc
    11521 atgcagatgc ggagctgaaa gagtatctgc aacagcttgg cgaacatcag accacgtcca
    11581 tcggctcttc gctgaaattc tgcctggtgg cggaaggaca ggcgcagctg tacccgcgct
    11641 tcggaccaac gaatatttgg gacaccgccg ctggacatgc tgtggccgca gctgccggag
    11701 cgcacgttca cgactggcag ggtaaaccgc tggattacac tccgcgcgag tcgttcctga
    11761 atcctgggtt cagagtgtct atttattaat taatttatgc agcaggtcca tcacttgctg
    11821 cacctcttcc tgagtaagcg ccccgtcttt ggcccattgc acgcgaccgt ctttatccag
    11881 caccactaca gcggaacttt tttcatccag ctgccaggca ccgcgtgcga cgccattgct
    11941 atcaacaata aactgcgacc agggataaag ctttttatta ctctccagac tgctgcgcac
    12001 aaacatgccg gaacccggaa ttgcgtcgtc ggtgttaaca atggtggtgg tctggtaacg
    12061 atcgtgcggt aacttcgctg atttaatcgc ttcaatcagc gtcgcgtttt tctcttttgc
    12121 agaggtgcga ccagcaatat gttgcagtac tcgcactttt cccactaact gcgcgctgtt
    12181 ccaggttttg tagctaaact gatctttatc aagcaccaac tcgccacgat cggtgatgcc
    12241 aatcggcggc actcgctgac cggtttcgaa ctgatgtgcg gaagccatca tcggcaacag
    12301 caggcaggtg agtgccagaa tcttgcgtag ggtcatggtg tttccttctt atgatatgca
    12361 ggtgatccga ccacttgggt ctgagtttta atcatatgtg ctatttatcg aatttcccgc
    12421 aagtgtgatg ccagtttgcg gtcaagcgca caaatcatat gaaaaatgaa tgcttatact
    12481 gaagaccgcg cttcggtaaa aagataattc tgaataattg taacctttag gtaaaaaaag
    12541 ttatacgcgg tggaaacatt gcccggatag tctatagtca ctaggcatta aaatttgcgc
    12601 ctcataatag ttgggccgat tgtggcaccg cacaggcgta atactcagca ggagataaca
    12661 atgaaaattt tccaacggta caacccactt caggtggcga agtacgtaaa gatcctgttc
    12721 cgtggacggt tatacatcaa ggacgttggc gcttttgaat ttgataaggg taagattctt
    12781 atcccaaaag tgaaggataa gctgcatttg tcagtgatgt ccgaagttaa ccgtcaggtt
    12841 atgcgtctgc aaacagagat ggcttaacca aagtgctatg cagtaataag acggctcctg
    12901 attcaggagc cgttgatgtt tctgggggtt acgcgacgct ttcttcttta tctttagcgt
    12961 caggcagttt tggcgaaagg gcggtggcct tgctgtcgat ccgggtcacc agcagctggt
    13021 cgatgcggta gttatcgata tccaccactt caaatttgta gccggcgaat ttcaccgaat
    13081 cggtgcgttt cgggatctta cgcagcataa acatcataaa gcctccgatg gtttcgtagt
    13141 tgcccgactg cgggaactcg tcaatatcca gcacgcgcat gacgtcgtca attggggtgc
    13201 cgccgtcaat cagccatgag ttctcatcac gggcgacaat ctgctcttcc agcccctgac
    13261 cgaccagatc gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc cccaccacca
    13321 gcgcgtactc gttcatgatc accgcgaagt cttcacctgc ggttttaaaa ctttccaacg
    13381 cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt gcgaatttgt acgccgctgt
    13441 tcagtgccag gctttggtta gccagcacgc ggttcagcag gtctttagaa tcgacataac
    13501 cgatgatgtg gtcaatatct tcattacaga cgaggaactt agagtgcgga tgttccgcca
    13561 ccttattctt caggctttgc tcatcttcgt ggagatcaaa ccaaatcacg ttttcacgcg
    13621 gtgtcattga agacggaacg gtacgggatt ccagctcaaa gacgttttca atcagctcgt
    13681 gttcctgttt acgtaacacg cccgccagcg caccggcttc cactaccgcg tagatgtcat
    13741 cagaagtgat gtcatcttta cgtaccattg gcagtttgaa aatacggaag atcatgttcg
    13801 ccaggccgtt gaagaaccac accagcgggg tgcaaacgta caggcagaag cgcatcgggt
    13861 tgatgatacg caaagccacc gcttctggcg caatcatacc gatgcgtttc ggggttaaat
    13921 ccgcaaacag gataaacatg ccagtcacta acgagaaaga gagaataaag ctcagttgct
    13981 cagagagttc tgccgacata tagcgggaga acaggctgtg aaaagctgga gaaaatgccg
    14041 catcaccgac gataccgccg agaatcgcaa ctgcgttcag accgatttgg accaccgtaa
    14101 agaacatgcc gggattttcc tgcatattca gaacgcgttg ggcatttata ttgccttcat
    14161 cagccagcag tttaagtttg attttgcgtg aggcggcaag tgagatctca gacatcgaga
    14221 agaacgcact tacagcgatc aagcagagta tgactaaaat actgtttaac atatcttatc
    14281 cgacctttca ggccggatcc tcggaagggg aagttgatta atttgtgtga aatacacatt
    14341 gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc gtaaggtact
    14401 gtaaagtcgc cagagggtta aaattcagac tgctgataaa tgcaatactg cctgatgcgc
    14461 tatgccgatc aggcctacgc agtccctgca atatattgaa tttggacgat tttgtaggcc
    14521 tggtaaggca ttcacgccgc attcggcata aacaaaacgc actttatcaa caatctacat
    14581 ttgtacccgt aacgctatgc ttccggtggc agacagacgc caattccgcc aattccacag
    14641 taaccatacg ggtttttatg cagatattgc tggtggtcat cttcggcata ataaaacggt
    14701 gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat cggcggcaag catcgccgcc
    14761 tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt ctggggtcag cggataaatc
    14821 gctgaacgat actgcgtgcc gtggtcattg ccctgacgca ttccctgggc gggatcgtga
    14881 ttctcccaaa atacctgtag caactgctca tagctgatga cagaaggatc gtaaacaatg
    14941 cgtaccgctt cggcatgacc cgtatcaccg gagcacactt cccgataagt cggatttggc
    15001 gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg ccagaacaga
    15061 cgctccacac cccagaaaca acccatcgca aaaatggcaa tctccattcc gtcaggtaca
    15121 ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta cgggcatcgg ggtgttacgt
    15181 ccaggcaggg catcggcggg ggaaaccaga tgctttttat caaataaact catggtgtcg
    15241 ctctcccgaa aatcggtcat tggggttaag gttgtaacaa gaggcgtatt tgcacacaat
    15301 aaccaccgtg aataggtcta aagtaaaaca taagaaatat ttggggttta gtctgctttt
    15361 taatccatat tactggattt ttgttaagcc gtttaacggc gttccagggg caggaaaaaa
    15421 ggatattcag gagaaaatgt gcgctatatc cgacagttat gctgtgtaag cttactctgc
    15481 ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt atcgggacag
    15541 ctggaaaaga acgttcgtgc gcagctttct acgattgaaa gtgatgaagt gacgccagac
    15601 cgtcgctttc gcgcacgcgt cgatgatgcc atccgcgaag gtctgaaagc gctgggttat
    15661 taccagccga ccattgaatt tgatctccgt ccaccgccaa agaaagggcg gcaggtattg
    15721 atcgccaaag tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt ggtattgcgc
    15781 ggcggcgcgc ggaccgataa agactatttg aaattgctcg atactcgccc ggctattggc
    15841 acggtactga accagggcga ttatgaaaat ttcaaaaagt ccttaaccag cattgcgttg
    15901 cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc tgggcattgc gctcggcctg
    15961 cataaagcct tctgggatat tgattataac agtggcgaac gttaccgctt tgggcatgtg
    16021 acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc gtttaaagag
    16081 ggcgatgagt acgaatcgaa agatctggcg gaacttaacc gtcgactttc tgctaccggc
    16141 tggtttaact cggtcgtagt ggctccacaa tttgataaag cgcgcgaaac gaaagtatta
    16201 cccttgacgg gcgtggtttc gccgcgaact gaaaacacca tcgaaaccgg ggtcggttac
    16261 tctacggacg tgggaccgcg cgtgaaagct acgtggaaaa agccgtggat gaactcttat
    16321 ggtcacagtc tgaccaccag taccagtatt tccgcgccgg aacagaccct cgacttcagc
    16381 tataaaatgc cgctgctgaa gaatccactg gaacaatatt atttggtgca gggcggtttt
    16441 aagcgcactg acctgaacga taccgaatct gactccacta cgctggtggc ttctcgctac
    16501 tgggatctct ccagcggctg gcagcgtgcc attaacctgc gctggagtct cgaccacttt
    16561 actcagggtg aaattaccaa caccacgatg ctgttttatc ctggggtgat gattagccgc
    16621 acgcgttctc gtggtggcct gatgccaacc tggggcgact cgcaacgcta ctctatcgac
    16681 tactccaaca cggcctgggg ctcagatgtc gatttctccg ttttccaggc gcagaacgtc
    16741 tggatccgca cactgtacga tcgccatcgt ttcgttacac gcggcacgct gggctggatt
    16801 gaaaccggtg atttcgacaa agtaccgccg gatctgcgtt ttttcgccgg gggcgatcgc
    16861 agtattcgcg gctacaaata caaatctatc gccccgaaat acgccaacgg tgacctgaaa
    16921 ggggcctcga agttgataac cggatcgctg gaataccagt acaacgtgac cggaaaatgg
    16981 tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg atattcgccg cagcgacttt
    17041 aaaaccggta ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat caaactcgat
    17101 tttgccgtac cggtcgcgga taaagacgaa cacgggttac agttttacat cggtctgggg
    17161 ccagaattat gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt atcttactgt
    17221 tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg cctgcatctt gtatttaaag
    17281 cggcggatcg ctgggtgcca ggactggata ttggcaaggt caccggcggc tggcgcgatc
    17341 tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc ggtaaaagcg ggtaatctgc
    17401 atctggcggt cggtcttgag tgcctgtgga acagtagcgt ttgtattaat gacctggcgc
    17461 tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa caggttgaag
    17521 aagaggaaga tagcggtccg ctggatctct ccacgccgta tcccatcacc ctgacacggg
    17581 tggcgctgga caacgtcaac atcaagattg atgacaccac ggtgtcggtg atggacttca
    17641 cctccggcct gaactggcag gagaaaaccc tgaccctgaa accgacgtcg ctgaaaggcc
    17701 tgctgattgc tctgccgaaa gtggcggaag tggcgcagga agaagtggtc gaaccgaaaa
    17761 ttgaaaatcc gcagccggag gaaaagccgc tcggcgaaac gctgaaagat ctcttttctc
    17821 gcccggtatt gccggaaatg accgacttgc atttgccgct taacctgaac attgaagagt
    17881 ttaaaggcga gcagttacgc gtgacgggcg acacggacat caccgtgcgc accatgctgc
    17941 tgaaagtgag cagcattgac ggcaatacta aactggacgc cctggatatc gattccagtc
    18001 aagggatcgt caacgccagc ggcacggcgc agctgtcaga caactggccg gtggatatca
    18061 ccctcaacag cacactgaac gtggagccgt tgaaaggtga aaaagtgaag ctaaaagtgg
    18121 gcggcgcgct gcgcgaacag ctggagattg gcgttaatct ttccggtccg gtggatatgg
    18181 atttacgcgc ccagacgcga ctggcggaag ccggattgcc actcaacgtg gaagtgaaca
    18241 gcaaacagct ttactggccg ttcaccggtg agaagcagta tcaggcggat gatctgaaac
    18301 tgaaacttac cggcaaaatg accgattaca cgctctctat gcgtacggca gtgaagggac
    18361 tggagatccc gccagccacc attacccttg atgccaaagg taatgaacag caggtcaatc
    18421 tcgacaaact caccgtcgcg gcgctggaag ggaaaactga actcaaggcg ttgctcgact
    18481 ggcagcaggc cattagttgg cgcggtgagc taacgcttaa cggcattaac accgccaaag
    18541 agatcccaga gtggccgtcg aaactcaatg gcttgattaa aacccgcggt agcctgtacg
    18601 gcggcacctg gcagatggag gtgccagaac tgaagctgac cggtaacgtc aaacagaaca
    18661 aagtgaacgt tgacggcacg ctgaaaggca acagttatat gcagtggatg atcccagggc
    18721 ttcatctgga actggggcca aacagtgccg aagtgaaagg cgagctgggg gtaaaagatc
    18781 tcaatcttga tgccaccatc aacgcgccgg ggctggataa cgcgctgccg gggcttggcg
    18841 gtacagcgaa agggctggtg aaagttcgcg gcacggtgga agcgccacaa ctactggcgg
    18901 acatcaccgc gcgcggcctg cgctggcagg agctaaccgt ggcgcaggtt cgcgtggaag
    18961 gcgatatcaa atccaccgtt cagattgcgg gtaaactcga cgtgcgcgtt gagcaaattt
    19021 cacagccgga tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc gaaaaacagc
    19081 acgagttaca gttgcggatt cagggcgagc cggtttccgg gcagcttaat ctggcaggaa
    19141 gttttgatcg caaagaagaa cgctggaagg gaactcttag caatacccgc ttccagacgc
    19201 cggtcggccc gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat aaggagcaaa
    19261 aaatcagcat cgggccacac tgctggctta acccgaatgc ggaactgtgc gtgccgcaaa
    19321 ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct caaccgcttc gacctcgcca
    19381 tgctgaaacc gtttatgcca gaaaccactc aggccagcgg tatcttcacg ggtaaagcgg
    19441 atgttgcctg ggacaccacg aaagaggggc tgccgcaggg cagtatcacc ctttcggggc
    19501 gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct gcccgtggcg tttcagacac
    19561 tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt gggctggacc atccgcctga
    19621 ccaataacgg ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc cgccgtaatc
    19681 ttggtggcaa cgtcaatatc cgtaacttca accttgcgat gataaacccc atctttaacc
    19741 gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg tctgggtggt gatgtgcaaa
    19801 gcccgcagtt gtttggtcag cttcaggtta cgggtgtgga tatcgacggc aactttatgc
    19861 cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa tggtatgcgc tcgacgcttg
    19921 ccggtacagt acggacccag cagggtgaaa tctacctgaa cggtgatgcc gactggagcc
    19981 aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg atcaccgtgc
    20041 cgccgatggt acgaatggat gtatcgccag atgttgtctt cgaggctaca ccaaacctgt
    20101 ttaccctcga tggtcgcgtg gatgtcccgt gggcgcgcat cgtggtgcac gatctgccgg
    20161 aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa cgataacctg caacctgaag
    20221 agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat tgtccacgtt ggcaacaatg
    20281 tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg cgatcttaac gtcgttcagg
    20341 acaaacaagg gctgggcctg aacgggcaga tcaacatccc tgaagggcgc ttccatgcct
    20401 atggtcagga tctgattgtg cgtaaaggtg agttactgtt ctctggtccg ccggatcaac
    20461 cgtatcttaa tattgaagct attcgtaacc cggatgctac agaagacgac gtaatcgccg
    20521 gagttcgcgt cactggtctg gcggacgaac cgaaagcgga gatcttctct gacccggcga
    20581 tgtcgcaaca agctgcattg tcttatttgc tacgtggaca agggctggag agcgatcaga
    20641 gcgacagtgc ggcaatgacc tcgatgctga ttggtctggg ggttgcgcaa agtggccaga
    20701 ttgtgggtaa aatcggcgag acgtttggcg taagcaattt agcgctcgac acccagggag
    20761 taggcgactc ctcccaggta gtggtcagcg gctatgtatt gccaggtctg caagtgaaat
    20821 atggcgtggg tatatttgac tctatagcaa cactcacgtt acgttatcgc ctgatgccta
    20881 agctatatct ggaagccgtg tctggtgtag accaggcact ggatttgctc tatcagttcg
    20941 agttttagca atgcgaatat ttgtctacgg cagtttacgc cacaaacaag gcaacagtca
    21001 ctggatgacc aatgcccagt tactgggcga tttcagtatc gataactacc agttgtatag
    21061 cctgggccac tatccaggcg cagttccggg gaacggaaca gtacacggtg aagtttatcg
    21121 tattgacaac gccacgctgg ccgaacttga tgccttgcgc accaggggcg gtgaatacgc
    21181 gcgccagttg attcagacgc cgtacgggag tgcatggatg tacgtttatc aacgacccgt
    21241 cgatggatta aagctaattg aaagcggcga ctggttagac agggataagt aaccatatgc
    21301 atacgccacc ttcgggtggc gttgtttttt gcgagacgac tcgcattctg ttttgtaatt
    21361 ccctcaccct ttgcttttct ctccgagccg ctttccatat ctgttaacgc ataaaaaact
    21421 ctgctggcgt tcacaaatgc gcaggggtaa aacgtttcct gtagcaccgt gagttatact
    21481 ttgtataact taaggaggtg cagatgcgta ttaccataaa aagatggggg aacagtgcag
    21541 gtatggtcat tcccaatatc gtaatgaaag aacttaactt acagccgggg cagagcgtgg
    21601 aagcgcaggt gagcaacaac caactgattc tgacacccat ctccaggcgc tactcgcttg
    21661 atgaactgct ggcacagtgt gacatgaacg ccgcggaact tagcgagcag gatgtctggg
    21721 gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa agtgaatttg aacggggaga
    21781 cattgtgctg gttggctttg atccagcaag cggccatgaa cagcaaggtg ctggtcgacc
    21841 tgcgcttgtg ctctccgttc aagcctttaa tcaactggga atgacgctgg tggcccccat
    21901 tacgcagggc ggaaattttg cccgttatgc cggatttagc gttcctttac attgcgaaga
    21961 aggcgatgtg cacggcgtgg tgctggtgaa tcaggtgcgg atgatggatc tacgcgcccg
    22021 gctggcaaag cgtattggtc tggctgcgga tgaggtggtg gaagaggcgt tattacgctt
    22081 gcaggcggtg gtggaataag gtgtgtttat ttatcgcggg cataaaaaaa cccttactaa
    22141 ccgaagctcg gcgttcaggg ttattgcgct agaagaactt atttattctt tgcgcgctcg
    22201 aaggaagcaa cgatttcagc tttagcggct tctgcgtttt cccaaccttc aactttcacc
    22261 cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt gagcgatctg cgctttcagc
    22321 agctctggca gatcgttaac gtctttaatg tgatcgtatt ctttgctcag cttgctgtgc
    22381 ggaaccgcaa ccagtttcgc atcttcaccg gcttcgtcgg tcattttcag aacgccaacc
    22441 ggacggcaac ggatcacaga acccggctgc agcgggtacg gagtcgggac cagtacgtca
    22501 accgggtcac cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca cggatagaac
    22561 atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc gatttcgtat
    22621 ttgatcggat ctgcgttagc cgggatctca ataacaacgt agatgtcttc cggcagatct
    22681 ttacccgcag ggacgttgag taagctcatg tctgtttcct ttaaaaatat gtggcaaaca
    22741 agtgccgagt attatagcca actcgcgccg aatgtctttg cttgttttcg tcttcatttt
    22801 cctttttcgc cagttttaag acggtataca gagcaggaaa atccataacc gtagccgcat
    22861 ttttcatagt gagatgaaag cgattacaaa cttgtgatta acgttttatt tacttttttg
    22921 aagtgtgatg taacgcaatc cgttacataa cgaattgtct atagtttttt cgcgaacatc
    22981 ttttcaccaa taataattac cccgacgagg acaaccctat gtggaaacgc ttacttgtag
    23041 tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc cgctccatta accgtaggat
    23101 tttcgcaggt cggatcggaa tccggctggc gcgctgcaga aaccaatgtg gcgaaaagtg
    23161 aggccgaaaa acgcggaatt acgttgaaaa ttgccgatgg tcagcaaaag caggaaaacc
    23221 agattaaagc ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt atcgctccag
    23281 tggttgcgac gggttgggaa ccggtattaa aagaggcgaa agatgccgaa atcccggtct
    23341 tcttgcttga ccgttccatc gatgtgaaag acaaatctct ctatatgacc accgtcactg
    23401 ccgacaacat cctcgaaggc aagttgattg gtgactggct ggtaaaagaa gtgaatggca
    23461 aaccatgcaa cgtggtggaa ctgcagggca ccgttggggc cagcgtcgcc attgaccgta
    23521 agaaaggctt tgccgaagcc attaagaatg cgccaaatat caaaatcatc cgctcgcagt
    23581 caggtgactt cacccgcagt aaaggcaaag aagttatgga gagctttatc aaagcggaaa
    23641 acaacggcaa aaacatctgc atggtttacg cccataacga tgacatggtg attggtgcaa
    23701 ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga tatcctcacg ggttccattg
    23761 acggcgtacc ggatatctat aaagcgatga ttgatggcga agcgaacgcc agcgttgaac
    23821 tgacgccgaa catggcaggc cccgcttttg acgcgctgga gaaatacaaa aaagacggca
    23881 ccatgcctga aaagctgacc ctgaccaaat ccacccttta tctgcctgat accgcaaaag
    23941 aagagttaga gaagaagaaa aatatggggt attgagggtt gctatgcctg atgccgattc
    24001 gtaggccgga taaggcgctc gcgccgcatc cggcgatggt gcactgaagc ctgatgcgac
    24061 gcttaccgcg tcttatcatg cctactggga gcacgcttta caccggggga aaccatgacg
    24121 accgaccaac accaggagat cctccgcacc gaaggattaa gtaaattttt ccccggcgtc
    24181 aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc gctgcttggt
    24241 gaaaacgggg cgggaaaatc aacgctaatc aaagcattaa ctggtgtata ccacgccgat
    24301 cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc ccatgcacaa
    24361 caactcggta tcggcaccgt ctatcaggaa gtcaacctgc tacccaatat gtcggttgct
    24421 gataatctat ttataggccg cgaacccaaa cgtttcggcc ttctacgccg taaagagatg
    24481 gaaaagcgcg ccaccgaact gatggcatct tacggtttct ccctcgacgt gcgcgaaccg
    24541 cttaaccgtt tttcggtggc gatgcagcaa atcgtcgcca tttgccgggc tatcgatctc
    24601 tccgccaaag tgctgatcct cgatgaaccc accgccagcc tcgacaccca ggaagtggag
    24661 ctattatttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat ctttgtcact
    24721 cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgtcttacg caacggcagt
    24781 ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa aatgatgctg
    24841 gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt gagcgacaaa
    24901 cccgttgccg cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt tgatctcgaa
    24961 gtgcgccccg gcgagatcgt cggtctggct ggcttgctgg gatccggacg taccgaaacc
    25021 gccgaagtga tcttcggtat caaacctgct gacagcggca cggcgttgat caaaggcaaa
    25081 ccgcaaaccc tgcgatcgcc acatcaggct tcagtactgg gcatcggatt ctgcccggaa
    25141 gacaggaaaa ccgatggcat catcgctgcc gcctcggtgc gggaaaatat catcctcgct
    25201 ctccaggccc agcgcggctg gctacgaccc atttcccgca aagagcagca agagattgcc
    25261 gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc gattgaattt
    25321 ctctctggcg gcaatcagca aaaagtgttg ctttcacgtt ggctactgac acgaccgcaa
    25381 tttctgatcc tcgacgagcc aacccgcggc attgatgttg gtgcccacgc cgagatcatc
    25441 cgcctgattg aaacgctgtg tgctgacggt ctggcgctgt tggtgatctc ctccgaactg
    25501 gaagagctgg tgggctatgc cgatcgggtg attatcatgc gcgatcgcaa acaggtggcg
    25561 gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc ggcgtaagga
    25621 gaacagtgtg atgcctcaat ctctcccgga cactacgccg ccgaaaaggc gctttcgctg
    25681 gccaacggga atgccgcagc tggcggcgct attgctggtg ctgctggtcg atagcctggt
    25741 agccccgcat ttctggcagg tggtgcttca ggatgggcgt ttgttcggta gccctatcga
    25801 cattcttaac cgtgcagctc ccgttgcgtt actggcgatt ggtatgacgc tggtgatcgc
    25861 caccggtggg attgatctct ccgtaggggc ggtgatggct atcgccggag ccacaacggc
    25921 tgcgatgacg gtcgcgggat tcagcctgcc gattgttttg ttaagcgccc tgggcaccgg
    25981 catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc agccgtttgt
    26041 cgccaccctg atcctgatgg tcgccgggcg cggcgtggcg caactgatca ccgccgggca
    26101 gatcgtcacg tttaactcgc cggatctctc atggtttggc agcggatcgc tgttgttcct
    26161 gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt tgacccgcaa
    26221 aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg caaaaaatgc
    26281 cggggtaaac acgcggatca tcgtcatgct cacctacgtg ttgagcgggc tgtgtgcggc
    26341 gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaata acgccgggtt
    26401 atggctggag ctggacgcca ttcttgcggt ggtgattggc ggcggatcgc tgatgggcgg
    26461 gcgctttaac ctgctgctct cagtcgtggg ggcgctgatt attcagggga tgaacaccgg
    26521 aattttactt tctggctttc cgccggagat gaaccaggtc gtaaaagcgg tggtggtgct
    26581 ttgtgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag tacgtagcca
    26641 tgataaaacg taacttgccg ctgatgatca ccatcggcgt ctttgtgctg ggctatctct
    26701 actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat atcctgaccg
    26761 ataacgcctt tcttgggata atcgccgttg gcatgacctt tgtgatcctc tctggcggta
    26821 tcgatctctc cgtcggttcg gtgatcgcct ttactggcgt gtttctggca aaagtgattg
    26881 gcgatttcgg cctctcgccg ctgctggcgt ttccgctagt gctggtgatg ggctgtgcct
    26941 ttggcgcatt tatggggctt ttgatcgacg ccctgaagat cccggcattt atcattaccc
    27001 tggcaggaat gttctttttg cgcggcgtca gctatctcgt ttcagaagag tcgatcccga
    27061 taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct ggcggcggtc
    27121 gcttaagtgc gatgggactg ctgatgctgg cggtggtggt tatcggcata ttcctcgcgc
    27181 atcgtacccg ttttggtaat caggtatacg ccattggcgg caacgcaacg tcggcaaacc
    27241 tgatggggat ttctacccgc agcaccacta ttcgcattta tatgctctcc accggactgg
    27301 caacgctggc ggggattgtc ttctcgattt atacccaggc cggatatgcg ctggcgggcg
    27361 taggtgtgga actggatgct atcgcctcag tggtaattgg cggtacgctt ttgagcggtg
    27421 gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcaggggcta attcagactt
    27481 atatcaactt tgacggtacg ctgagttcct ggtggacgaa aatcgccatc ggcattttgc
    27541 tgttcatttt tattgcctta cagcgtggat taacggtgct gtgggagaat cgtcagagtt
    27601 cgccagtgac aagagtcaat attgcgcagc gataaaaacg cctctccgtg tggagaggcg
    27661 caggagatta cgcgtccggg aattcacgga taaagcgttc gacatcttca accatatggt
    27721 cgttgccgac aaagaacgaa cggcgctggt gcagagtttc cgggatgata tccagaatac
    27781 gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc atcgggttgc
    27841 actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg tagagataaa
    27901 taccgccttt cagcaggtta cggtggaaat ccgcgaccag tgaaccgata taacgtgagg
    27961 tatacgggcg gttggtggat ttatcttctt cctggcagaa tttaatgtac ttcttcaccc
    28021 cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct ttctccggga
    28081 agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt
    28141 gaacaccgca tccggtggtg taaaccagca tggtagagga gccgtatacc acgtaacctg
    28201 ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccaacag
    28261 gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg
    28321 acgagccatc cagggggtcc atcagcacca cgtattttgc gtgttcacag ccttcaaaga
    28381 cgacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt gctttcagtg
    28441 cggctttcag tttttcatta gcgaacaagt cgagtttctg ctgaacctcg ccctgcacgt
    28501 tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga
    28561 taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag
    28621 aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc
    28681 aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat gttgcgctca
    28741 ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact tgcccgacat
    28801 atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt catattttag
    28861 gaatttgtgg cacatttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag
    28921 taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca
    28981 ttgagctgat tcagggttac gatgccagcc agctcgatcc gcagccggat ctggtgatta
    29041 ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc
    29101 cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg
    29161 ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg attctggaac
    29221 agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg
    29281 cgcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac tgcgccttct
    29341 tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc aacaaccttg
    29401 agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag ttccaccatc
    29461 tggtgcgtat cgttccgggg cagggccgta ttatctggcc ggaaaatgac atcaacctga
    29521 aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggtgag cagggtcact
    29581 ggcaggcgaa aaagctgacc actgacgctt ctgaatggga agtcttgctg gatggcgaaa
    29641 aagtgggcga agtgaaatgg tcgctggtgg gcgaacataa tatgcacaat ggcctgatgg
    29701 cgattgcggc ggctcgccat gttggtgtag cgcctgcaga tgccgctaac gcgctgggtt
    29761 cgtttattaa cgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc gtgacggtat
    29821 atgacgattt tgcccatcac ccgacggcaa ttctggcaac gcttgcggcg ctgcgtggca
    29881 aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat accatgaaaa
    29941 tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa gtcttcctgc
    30001 tgcaaccggc gcatattccg tggcaggtgg cagaagtggc agaagcctgc gttcagcctg
    30061 cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc gctcagcctg
    30121 gcgaccatat tctggtgatg agcaacggcg gttttggtgg gatccatcag aaactgctgg
    30181 atggtctggc gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg atatagtgtg
    30241 cgtaggccgg ataaggcgtt cacgccgcat ccggcatttg agcatagtgc ctgatgcgac
    30301 gcttgatgcg tcttatcagg cctacaggtg caccgcatcc ggcaaaccat actcacatca
    30361 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
    30421 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agagtacgca
    30481 gctgctgacg atcggcatcg tgccacagat ttaatacctc agcgatggcg tcatcacctt
    30541 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
    30601 gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc tggcgcagca
    30661 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
    30721 caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
    30781 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
    30841 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
    30901 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
    30961 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
    31021 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
    31081 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
    31141 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
    31201 cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg catcatccac
    31261 cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata ttgcccgtta
    31321 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
    31381 agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
    31441 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatt accaataccg aaggtggcag
    31501 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
    31561 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
    31621 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
    31681 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
    31741 gaaagcgcca gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
    31801 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
    31861 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
    31921 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
    31981 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
    32041 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
    32101 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
    32161 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
    32221 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
    32281 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
    32341 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
    32401 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
    32461 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
    32521 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
    32581 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgaaga
//
LOCUS       DAZFKM010000045        32305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFKM010000045 DAZFKM010000000
VERSION     DAZFKM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHKAY_002188"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHKAY_002188"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO5700863.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHKAY_002189"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHKAY_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO5700864.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHKAY_002190"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHKAY_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO5700865.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHKAY_002191"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHKAY_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO5700866.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="ACHKAY_002192"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="ACHKAY_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO5700867.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..7380
                     /gene="bcsA"
                     /locus_tag="ACHKAY_002193"
     CDS             4756..7380
                     /gene="bcsA"
                     /locus_tag="ACHKAY_002193"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO5700868.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPRPLDPVRYLIQTCWLLIGASRKETPK
                     PRRRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTF
                     SLILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWW
                     RYTSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPS
                     VDIFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIA
                     RTTHEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPH
                     HFFSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGG
                     IAVETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFR
                     LDNPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIAL
                     FVLPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAK
                     GGLVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLI
                     VLGGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQ
                     ILEGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTW
                     ALWQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPR
                     RPERSETAQPSDQALAQQ"
     gene            7391..9730
                     /gene="bcsB"
                     /locus_tag="ACHKAY_002194"
     CDS             7391..9730
                     /gene="bcsB"
                     /locus_tag="ACHKAY_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO5700869.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9737..10843
                     /gene="bcsZ"
                     /locus_tag="ACHKAY_002195"
     CDS             9737..10843
                     /gene="bcsZ"
                     /locus_tag="ACHKAY_002195"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO5700870.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10825..14298
                     /gene="bcsC"
                     /locus_tag="ACHKAY_002196"
     CDS             10825..14298
                     /gene="bcsC"
                     /locus_tag="ACHKAY_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO5700871.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14380..16368
                     /gene="hmsP"
                     /locus_tag="ACHKAY_002197"
     CDS             14380..16368
                     /gene="hmsP"
                     /locus_tag="ACHKAY_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO5700872.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16551..17837
                     /gene="dctA"
                     /locus_tag="ACHKAY_002198"
     CDS             16551..17837
                     /gene="dctA"
                     /locus_tag="ACHKAY_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO5700873.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18057..19553
                     /locus_tag="ACHKAY_002199"
     CDS             18057..19553
                     /locus_tag="ACHKAY_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5700874.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(19649..20578)
                     /gene="kdgK"
                     /locus_tag="ACHKAY_002200"
     CDS             complement(19649..20578)
                     /gene="kdgK"
                     /locus_tag="ACHKAY_002200"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO5700875.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20810..21577
                     /gene="pdeH"
                     /locus_tag="ACHKAY_002201"
     CDS             20810..21577
                     /gene="pdeH"
                     /locus_tag="ACHKAY_002201"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO5700876.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21647..23707
                     /locus_tag="ACHKAY_002202"
     CDS             21647..23707
                     /locus_tag="ACHKAY_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO5700877.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23941..25263)
                     /locus_tag="ACHKAY_002203"
     CDS             complement(23941..25263)
                     /locus_tag="ACHKAY_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5700878.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25674..26687)
                     /gene="yhjD"
                     /locus_tag="ACHKAY_002204"
     CDS             complement(25674..26687)
                     /gene="yhjD"
                     /locus_tag="ACHKAY_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO5700879.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26736..27635)
                     /locus_tag="ACHKAY_002205"
     CDS             complement(26736..27635)
                     /locus_tag="ACHKAY_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5700880.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            28155..28757
                     /locus_tag="ACHKAY_002206"
     CDS             28155..28757
                     /locus_tag="ACHKAY_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO5700881.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28808..30457)
                     /locus_tag="ACHKAY_002207"
     CDS             complement(28808..30457)
                     /locus_tag="ACHKAY_002207"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO5700882.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30862..32259
                     /gene="ccp"
                     /locus_tag="ACHKAY_002208"
     CDS             30862..32259
                     /gene="ccp"
                     /locus_tag="ACHKAY_002208"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO5700883.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         7686 a         8156 c         8872 g         7591 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggcta tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gttttgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcactcacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ctagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcattattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgttctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc actcgctggc
     4441 tatcgtcaat gtggatgcca actgccatat ccgcctgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaata acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattgct gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt cggtgaatac cgcagtgatg cgctggcggc
     4681 agaagagata ctgacgctgg cgaactggtg cctgttgaac tattccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgccacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatccgcata tcaacgcctc gcgtccgcgt
     4981 ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca
     5041 tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca aaatattcgt
     5101 ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa aacacagcat
     5161 ctggatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt gatcctcggt
     5221 atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcagcc gtttaacccg
     5281 ctggcgcagt ttatcttcct gatgctgctg tggggggtag cgctgatcgt acggcggatg
     5341 ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc ttgccgttat
     5401 atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct ggtgtgcggg
     5461 cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg ctacttccag
     5521 gtagtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc gctgtggccg
     5581 tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa aaatactatt
     5641 tacgcctcgc tgggtatcga ctggccgaaa gacaagctga acatctggat cctcgatgac
     5701 ggcggtaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata tatcgcccgt
     5761 accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata tgccaaaggc
     5821 gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc gatcgttcct gcaaatgacc
     5881 atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agacgccgca ccacttcttc
     5941 tcgccggacc cgtttgaacg caacctgggg cgttttcgta aaacgccgaa cgaaggcacg
     6001 ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt cttctgcggt
     6061 tcctgtgcag taattcgtcg caaaccattg gatgaaattg gcggtattgc tgtcgaaacg
     6121 gtaaccgaag atgcgcacac gtctctgcgc ctgcaccgtc gtggctacac ctctgcatat
     6181 atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca tatcggtcag
     6241 cgtattcgct gggcacgcgg gatggtgcaa atcttccgtc tcgataaccc gcttaccggt
     6301 aaagggctga aattcgccca gcggctgtgc tacgtcaacg ccatgttcca cttcttgtcg
     6361 ggcattccac gactgatctt cctgactgcg ccgctggcgt tcctgctgct tcatgcctac
     6421 atcatctatg cgccagcgtt gatgatcgcc ctgttcgtgc tgccgcatat gatccatgcc
     6481 agcctgacca actccaagat ccagggcaaa tatcgccact ctttctggag tgaaatctac
     6541 gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat taacccgcac
     6601 aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta cgtcgactgg
     6661 gtgatctcgc ggccctacat cttccttgtc ctgctcaacc tggtgggcgt tgcggtaggc
     6721 atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt cagtatggtg
     6781 tgggtattct acaacctgat tgttcttggc ggcgcagttg cagtatcggt agaaagcaaa
     6841 caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc ccgcgaagat
     6901 ggtcacctct tctcgtgtac cgttcaggat ttctccgacg gtggtttggg gatcaagatc
     6961 aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa acgcggtcag
     7021 caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt tgggctgaaa
     7081 ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt tgcccgtgcg
     7141 gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga aagtctgctg
     7201 gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc gccttcttcg
     7261 gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc gtttattccg
     7321 cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc tcaacaatga
     7381 tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg ggatgagtgc
     7441 gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca atgctgagcc
     7501 agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgc cgggcgtgca
     7561 gggcgctgat gcgccagtcg tggcgcagaa cggtccttcg cgtgatgtga agctgacctt
     7621 tgcgcaaatt gcgccgccgc cgggcagcat ggtgctacgt ggcattaacc cgaacggcag
     7681 cattgagttt ggcatgcgca gcgatgaagt ggtgacgaag gcgatgctca acctcgaata
     7741 cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta aaggtttatc tcaatgatga
     7801 gctgatgggc gtgctgccag tgaccaaaga acagttgggt aaaaaaacgc tggcgcaaat
     7861 gcccattaac ccactgttta ttaccgactt caaccgtgtg cggctggagt ttgtcggcca
     7921 ttatcaggac gtgtgcgaaa acccggccag caccacgctt tggctggatg ttgggcggag
     7981 cagtggactg gatctgacct atcagaccct gaatgtgaag aatgacctgt cacacttccc
     8041 ggtgccattc tttgacccgc gcgataaccg tactaacacc ttgccgatgg tctttgcggg
     8101 tgcgccggat gttgggctgc aacaagcctc tgccattgtc gcctcgtggt ttggttcgcg
     8161 ttctggctgg cgtgggcaga acttcccggt gctctataac caactgccgg atcgtaatgc
     8221 cattgtcttt gcaaccaacg acaaacggcc ggacttcctg cgcgatcatc cggcggtaaa
     8281 agcccctgtg attgagatga ttaaccatcc gcagaatcct tacgtcaaac tgctggtggt
     8341 gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa ggtatcgctc agggtaacat
     8401 tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa ccgctgctac cgcgtaagcc
     8461 gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc acatttggcg aactgaaaac
     8521 ctatgaagaa cagttacaat ccagcggtct tgagccagca gcgattaacg tttcgctaaa
     8581 cctgccgccg gatctctacc tgatgcgcag taccggcatt gatatggata ttaattaccg
     8641 ctacaccatg ccgccggtga aagacagttc gcggatggat atcagcctga ataaccagtt
     8701 cctgcaatcc ttcaatctga gcagcaaaca ggaggcgaac cgcctgctgc tgcggattcc
     8761 ggtattacaa ggtttgctgg atggcaaaac agatgtctct attccggcgc tgaaactggg
     8821 cgcgaccaac cagctgcgct tcgactttga gtatatgaac ccgatgccgg gcggttcggt
     8881 ggataactgt attaccttcc agccggtgca gaatcatgtg gtgattggtg acgactccac
     8941 catcgacttc tcgaagtatt accacttcat cccgatgccg gatctacgcg cctttgctaa
     9001 cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa accatcaccg tgatgccgaa
     9061 agcgcctaac gaagcacaga tggaaacgtt gctgaatact gttggtttta tcggcgcaca
     9121 gacgggcttc ccggcgatta atctgacggt gaccgatgat ggcagcacca ttcagggcaa
     9181 agatgccgac atcatgatca tcggtggtat cccggacaaa ctgaaagacg ataagcagat
     9241 cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca ccgatgcgcc agaccccgtt
     9301 ccccggcatt gtgccggacg agagcgatcg cgcggcagaa acccggtcaa cgctgacctc
     9361 ttccggtgcg atggcggcgg tgattggctt ccagtcgccg tataacgacc agcgcagcgt
     9421 gattgcgctg ttggcagata gcccacgcgg ttatgaaatg cttaacgatg cggtgaacga
     9481 tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg atccgcgagt ccggtatcaa
     9541 cagcctacgt gttggcgacg tttattacgt aggtcatctg ccgtggttcg agcgcgtgtg
     9601 gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg gcggctatca gtgtgatatt
     9661 gctggcatgg gtactgtggc gtctgctgcg aattattagt cgtcgtcgtc ttaacccgga
     9721 taacgagtaa ttgaagatga atgtgttgcg tagtggaatc gtgacgatgc tgctgctggc
     9781 tgcctttagt gttcaggcag cctgtacctg gcctgcctgg gagcagttta aaaaggatta
     9841 catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca ccacctccga
     9901 agggcaaagt tacggcatgt tctttgccct ggcggctaac gaccgtgcag ctttcgataa
     9961 tattctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac gtttgcccgc
    10021 ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca attcggcctc
    10081 cgatggtgat atctggatgg cctggtcgtt gctggaggcg gggcgtttgt ggaaagagca
    10141 gcgttatacc gacatcggca gcgcgttgct aaaacgtatc gcgcgggagg aagtggtgac
    10201 ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg ggttttgctg aggataacag
    10261 ctggcgtttt aaccccagct acctgccgcc gacgctggcg cagtatttca cccgctttgg
    10321 cgcgccgtgg accacgctgc gcgaaaccaa tcaacgttta ttgctggaaa ccgccccgaa
    10381 aggtttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc taaaagccga
    10441 aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag gcatgatgcc
    10501 tgacagcgat ccgcaaaaag cgcggatgct caaccggttt aaaccgatgg cgacattcac
    10561 tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag cgcagggtaa
    10621 aggaccggtc ggtttttctg ccgccatgct gcccttttta caaaaccgcg atgcgcaggc
    10681 cgttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatgcctatt acaactatgt
    10741 gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga caaaaggtga
    10801 gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt cacgctttcc
    10861 ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccgctcagca acagttgctg
    10921 gagcaagttc ggttaggcga agcgacccat cgtgaagatc tggtgcaaca gtcgttatat
    10981 cggctggaac ttattgatcc gaataacccg gacgtcgttg ccgcccgttt ccgttctttg
    11041 ttacgtcagg gcgatattga tggcgcgcaa aaacagctcg atcggctgtc gcagttagcg
    11101 ccgagttcaa atgcgtataa atcgtcgcgg actacgatgc tactttccac gccggatggt
    11161 cgtcaggcac tgcaacaggc acgattgcag gcgacgaccg gtcatgcaga agaagctgtg
    11221 gcgagttaca acaaactgtt caacggtgcg ccgccggaag gtgacattgc tgtcgagtac
    11281 tggagtacgg tggcgaaaat tccggctcgc cgtggcgaag cgattaatca gttaaaacgc
    11341 atcaatgcgg atgcaccggg caatacgggc ctgcaaaaca atctggcgct attgctgttt
    11401 agtagcgatc gccgtgacga aggttttgcc gtcctggaac agatggcaaa atcgaacgcc
    11461 gggcgcgaag gggcctctaa aatctggtac gggcagatta aagacatgcc cgtcagtgat
    11521 gccagtgtgt cggcgctgaa aaaatatctc tcgatcttta gtgatggcga tagcgtggcg
    11581 gctgcgcaat cgcaactggc agaacagcaa aaacagctgg ccgatcctgc tttccgcgct
    11641 cgtgcgcaag gtttagcggc ggtggactct ggtatggcgg gtaaagccat tcccgaacta
    11701 caacaggcgg tgcgggcgaa cccgaaagac agtgaagctc tgggggcgct gggccaggcg
    11761 tattctcaga aaggcgatcg cgccaatgca gtggcgaatc tggaaaaagc cctcgcactg
    11821 gacccgcaca gcagcaacaa cgacaaatgg aacagtctgc tgaaagtaaa ccgctactgg
    11881 ctggcgatcc agcagggcga tgctgcgctg aaagccaata atcctgaccg ggcagaacgc
    11941 ctgttccagc aggcgcgtaa tgtcgataac accgacagtt atgcagtgct ggggctgggc
    12001 gatgtggcga tggcgcgaaa agattatccc gccgccgaac gttattatca gcagaccttg
    12061 cgtatggaca gcggcaacac taacgccgtg cgcgggctgg caaatattta ccgccagcaa
    12121 tcgccagaaa aagctgaagc gtttatcgcc tcgctctctg ccagtcagcg gcgtagcatt
    12181 gatgatatcg aacgcagcct gcaaaacgac cgtctggcac agcaggcaga ggcactggaa
    12241 aaccagggca aatgggcgca ggcggcagca cttcagcggc aacgactggc gctggacccc
    12301 ggcagcgtat ggattactta ccgactttcg caggatctct ggcaggccgg acaacgcagc
    12361 caggccgata cgttaatgcg caatctggcg cagcagaagc cgaacgaccc ggagcaggtt
    12421 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
    12481 aacagcctgc cgcgcgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
    12541 agcgatcagg tgctggaaac cgctaatcgc ctgcgagaaa gcggcaaaga agctgaagcg
    12601 gaagcgatgc tgcgtcagca accaccttcc acgcgtattg acctcacgct ggctgactgg
    12661 gcgcagcagc gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgctg
    12721 gagccaacta acgccgatgc cattctgggg ctgacggaag tggatattgc tgccggtgac
    12781 acagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
    12841 acacagcggc gcgtggcgct ggcgcaggcg cagcttggag ataccgcagc ggcgcagcag
    12901 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
    12961 gtgctgcgcg acggtgcgaa gtttgaagcg caggcgggcg atccaaagca ggcgctggaa
    13021 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
    13081 acattcaccc gactgacccg taacgacgag aaagatgact ggctgaaacg cggcgtgcgc
    13141 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcaccctcga gcacgattac
    13201 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
    13261 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
    13321 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
    13381 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
    13441 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
    13501 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
    13561 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    13621 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    13681 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    13741 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    13801 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    13861 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    13921 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    13981 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    14041 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    14101 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    14161 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    14221 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    14281 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    14341 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    14401 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    14461 accgttttgc tgttccatct ggtccaacag aatcgctata acacggctac gcaactggaa
    14521 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    14581 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    14641 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    14701 gtaatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    14761 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    14821 atgtataagt tcgtgatgag caccctctca atgttagtga ccatttactt acttttgtcg
    14881 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    14941 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    15001 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    15061 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    15121 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    15181 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    15241 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    15301 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    15361 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcactat catgagcgag
    15421 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    15481 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    15541 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    15601 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    15661 tggttacagc cacaggtcga gatgaccagc ggaaaactgg tcagtgcgga agtgttactg
    15721 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    15781 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    15841 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    15901 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    15961 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    16021 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    16081 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    16141 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    16201 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    16261 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcgc
    16321 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    16381 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    16441 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    16501 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    16561 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    16621 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    16681 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    16741 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    16801 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    16861 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    16921 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    16981 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    17041 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    17101 tcatcttcgg catcatcaat atgatcatgc gtctggcgcc tattggtgcg ttcggggcaa
    17161 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    17221 tctgtttcta cattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    17281 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    17341 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    17401 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    17461 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    17521 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    17581 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    17641 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    17701 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    17761 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    17821 acgaattatc ctcttaatct catcacttat gcccgcactc ccttccccga gtgcgggtgt
    17881 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    17941 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    18001 agactgaccg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    18061 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    18121 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    18181 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    18241 tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    18301 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    18361 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    18421 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    18481 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    18541 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    18601 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    18661 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    18721 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat aaacaaaacg tttggcgaac
    18781 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    18841 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    18901 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    18961 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    19021 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    19081 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    19141 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    19201 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    19261 tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    19321 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    19381 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    19441 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    19501 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    19561 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    19621 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    19681 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    19741 cactgccgcc cgtcaaacgt accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    19801 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    19861 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    19921 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    19981 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    20041 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    20101 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    20161 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    20221 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    20281 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    20341 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    20401 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    20461 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    20521 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    20581 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    20641 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    20701 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    20761 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    20821 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    20881 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    20941 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    21001 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    21061 gattgatttg ctggcgcaaa aagccgactt ctttatagag cacggcctgc tggcatcggt
    21121 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat
    21181 tgagcgtctt ccctggctgc gtttcgaact ggtggagcat atccgtctgc cgaaagattc
    21241 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gtaccgggat
    21301 ggcaaatttc tctgcactaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact
    21361 gtttgtgatg ctgcgtcagt cgccggaagg acgcacactc ttttctcagc ttttacatct
    21421 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacgccgg aagagtggcg
    21481 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    21541 gatagaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgcctggct
    21601 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    21661 caaaataacc gctgcgattt cagtggcttt cttgttgttg attgtcgtgg cgatcatttt
    21721 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    21781 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    21841 agaaactggc tggcgcagct gggtgccgtg gccccatgta cacgcggaag acatcattct
    21901 tggcaatcca ccggatattc ccgaagtcac gatggtgcat ttgccacgcg tagaggcaac
    21961 gctggccccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa
    22021 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatctcgc
    22081 caacgatgat aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    22141 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    22201 gatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    22261 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    22321 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    22381 agggacgccg tttccggtac aggctgattt ccgctctggt aacacccgtg ttgcttttga
    22441 tggcgtcgtg aatgatccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    22501 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    22561 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    22621 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    22681 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    22741 cctgggaccg ttaattggcg ttgactccgg gaaaggggca gaaaagtcga aacggtctga
    22801 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    22861 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    22921 gcatggcagt agcctgccga ttagtgatct ttctactcat atcatcctca aaaatgctga
    22981 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcgg cgaatattca
    23041 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    23101 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    23161 tgacgcggaa ctacgcggta gcggtaactc ggtggcggca cttttaggca acagtaacgg
    23221 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    23281 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gatgatgagg tgcgggtgaa
    23341 ctgcgcggcg gcgaatctgg atattgccaa cggcgtggcg cgcccgcaga tctttgcttt
    23401 cgatactgag aacgcgttga ttaacgttac cggcacggca agttttgctt ccgaacagct
    23461 ggatttgact attgatccgg agagtaaagg aattcggatt atcacactgc gttcgccgct
    23521 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccgggc cgttgattgc
    23581 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccggcggcgg cgttactggc
    23641 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    23701 gaagtgatga ggttggattg tgtttgctgg tgagttcggg agcagcgtgt tcccctcacc
    23761 ctaaccctct ccccaaaggg gcgaggggac tgttttgtgc gtgttgaaac aacgagtacc
    23821 gcttttccgt tcactttctg ggggagggct gttatgttcg cgatataaca tctcgctccg
    23881 cttttctccc tctccctttc agggagaggg ctggggtgag ggtggctgcc cacacgatac
    23941 ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg tcaagccagc
    24001 catcgccgcc agatataacc ccaccgcacc taacccgtag ttagcctgca accaggctgc
    24061 gatatatggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga acgatgctcc
    24121 ggtgtaacgc acttctgtcg ggaacagctc tggtaacagc gcgcccatcg gcccgaaggt
    24181 cagtcccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg ggttgccaga
    24241 accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg tgatgattac
    24301 catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca ccatcacgcc
    24361 aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca ggccaagccc
    24421 aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt aaaacagcgt
    24481 ataggttgcc agcataatga acgtacccag tacggttacg cgaacatgtt tggtcagcag
    24541 cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa acaccggcga
    24601 ctcatgcagc gacacgcgaa catacaggcc gataatgacc agcaccgccg agaagataaa
    24661 tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg aaagcagcaa
    24721 gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga aggagccata
    24781 cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc cgccccattc
    24841 accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg gggcgaaaat
    24901 accaatcgtg gcatagcccg gcagcagacc aatcaccacg gtcgaaatcc ccatcgttag
    24961 caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac caaaaacggc
    25021 agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact gtagcgttgc
    25081 tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg ccgcagtggc
    25141 gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga caaggacttt
    25201 attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg ctgttgcttg
    25261 cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc aaaagtgata
    25321 tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg gataatgttt
    25381 aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag tggataaaac
    25441 tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta tgagttggaa
    25501 tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt ttcaagaggt
    25561 tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta cggggagcca
    25621 ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct attttaaggc
    25681 tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat ccacgccgcg
    25741 caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag cacggaacca
    25801 aatgctgcgc cagaggggga tttcatcagc gatggcaagg tgtaggtcat cacgatttta
    25861 atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt tttgcgcgga
    25921 cggtggcgcg gcaaccgcca gaagatccag aagaaaagca gatagttggc gaagatggaa
    25981 atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt caggtgcagg
    26041 gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgacag cgtcacaatc
    26101 agcgcaatta acaaaccaat cagtgaaata aaatcacgca gatattttac ccagaacttc
    26161 tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcacgaat cgcttcacgc
    26221 aggttaccca tccagttgat gccggaataa agtgccaccg ccaggccgac aagccctaca
    26281 gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc tagcgtcgga
    26341 tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg atgggaggcc
    26401 agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga caaaaatgag
    26461 aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg ttctgtcgca
    26521 cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt ggtgacggtt
    26581 tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgttt aatcggctcg
    26641 tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg ctcttttgtc
    26701 cttctgtcgt ggtcattctt aaaaagtata gtcggtcagt ccaggtgctc tttcattacg
    26761 ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg ctgcgggtaa
    26821 accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat aagccgcccg
    26881 gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag accggcaaga
    26941 ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga cttaaaccac
    27001 tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag gtgcggtgta
    27061 taacgcacta ttgcgtgtga agtcagatca tcggggcttt gcggataacc aaagcgcgtc
    27121 agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg ggcgataacg
    27181 ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg aagaatatct
    27241 accggacggt catggctact cagttccagc tcaattcccg gatgcagata gagaaattcc
    27301 gacaggcgcg gcagtaacag gcttttcgcg attcctggcg ggatgtcgat gcgtaattta
    27361 ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag attactcaac
    27421 acatcttttg ctcgttgata ataggtcatg ccttctggcg tgagtttgac ccgtcgcgtg
    27481 gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg gcgcgaaaca
    27541 cttccttttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag ctccgcgact
    27601 ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg ttgttcacag
    27661 cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta atgccgggtt
    27721 tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg gaatagcttt
    27781 ctgagaaagg tgaatccccc taaacagagc gtatttttca gcgattccct ttaaagtgaa
    27841 tagaggacgt gttggctatt tgtgcttatt tttgtctcat tgccgtttgt tatttttgct
    27901 tgtggtcgcg gagtttcatt tttaattgca tgcgtactaa ccaccaatat aaggaaatcc
    27961 tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc tgtaactatt
    28021 tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc aaatggcgaa
    28081 ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta acgtattcag
    28141 gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt attcatggaa
    28201 tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc agtcaggcag
    28261 acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc gatggcgatc
    28321 aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt cctgaggtta
    28381 aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt atccatttta
    28441 atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg gcggtaaata
    28501 gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg gaaaaggaca
    28561 ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg ttagcggcgg
    28621 gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg gtaaaagcgc
    28681 atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg gcaatgatgt
    28741 taaatatctc ctcctgaaga ggataatttt acagccagca ggaagttagc ggctggctgt
    28801 aaaaatatta tggttcgccg tacaaaccaa ttaaacggcg taccacaccg ttagtccagc
    28861 caaacccatc ctgcaacgga tactcgccac cgccgccttc gcggggaaca ccatcggcaa
    28921 tatggtattt ctcgatcatt ttgtgctgtt ccagatagaa ctgattcacc gttttcagcc
    28981 agctgcgcgc gatttcatca cccagaaggt catcgccata cattttaaat ccctgaattg
    29041 ccatccattg taacggtgcc cagccattgg gtttatccca ctgttcaccg gtttcgtact
    29101 cgcttgccag aatcccgcca ggtgtcagta accggctgcg tacggcgttt gccagacgat
    29161 cggcctgttc atggttcgcc atgccgacat agagcggcac aatggcggca gcggaaaaca
    29221 gcgccagttg ttcgcgtcgc cagtcgtaat cgcggtagat gccgttttca tcatcccaga
    29281 ggtaacggtt taccgcatcg cgacgggcac tggccttctg gcggaacagc gcttctgtct
    29341 ctttctcgcc tttcagcgcc gagatgttgg cgatggcgct ctccagttta aacaggaagg
    29401 cattcagatc gatggggatg aactgggtgg tacgaatgct cgccagacga ccagtatcac
    29461 gcagccaacg ggaagagtaa tcccaacctg aggccgctcc cgcgcgtaaa tcgcggtaca
    29521 cctcgttggg cgggcgacca gaatgtttcg cggtttcaac gtcctcaagc caggattcgt
    29581 cacgcggcgt gtcgcgatcg tcccaataac ggttgagcag cgatccgtcc ggcatccgca
    29641 caacatggcg ataggcctga ttagggatca acgattctgc accgtccatc cagaaggcat
    29701 attccatttt aaggtggtcg agatagcggc gcgcaccgcg tacaccatct tcttcaaaca
    29761 actccaccat cagcgcaaaa accggtggtt gcgatcggct caaataatag gtgcggttgc
    29821 cgttggggat gtgaccatag ttttcgatca tccaggcgaa gttatcggcc atgcatttca
    29881 gcaaatcttc ccgaccactt tccgccagcc ccagcatggt gaaataggaa tcccaatagt
    29941 aggtttcgct aaaacggccg cccgggacaa tatatgactg cggtagcgcc agcagagaag
    30001 accacggaat gtgatcctgt ggttcgcggg ttagcaccgg ccacagctgg tcgatatgct
    30061 ctttcaggga attttgcggg tccgatacat actcgctgga gtagacctcc ggcagccaga
    30121 agtgattttc aacaaacttg cgcaagtcaa aatcacgatg acggcgcact ttacggtagc
    30181 ggattaaaat atccagcggg tccattttgg gtgcgcagtc gggaaaggtt ttactgtcgg
    30241 ggaaaatttt cgccgactga acatgctcga acagttcgag atagcgatcg gcaggcgtca
    30301 gcgcatcaga ggcgggcagc ccttcaatca tctcgggttc cggttctgcc tcgatcatct
    30361 catccagttt taattcatac gggtccagtt catagcagag atcgacctcg atcatcagtt
    30421 cgtctggatt agggttttga attttctgat tgagcataag gatccaacct ccgaaattcg
    30481 tcgctaatta tcgggctttt ctttaagggt agtcattcgc tccgcctggt gggcgacaaa
    30541 gcgtgcggtg gatcacgttt attaacttgt caaaaaatat aacgcactaa tatcaatgga
    30601 atttataaaa atacggattt cgtgttgttt ttcattttca gaacattcaa tcctgctctc
    30661 gccgcaatca tgatatgcca ttcctgatct gggtaatagg cacaggctat cttattgata
    30721 gtttatattc atgtaattga ttgattccta tcaaaaagcg tatctccctg ttttttatga
    30781 tggctttgtc tcgcagagac gtttgcagca ttctggaaaa ttttctgggt cacaataact
    30841 caccaccgtg agggaagaat aatgaaaatg gtctcacgta ttaccgcgat cggcctggct
    30901 ggcgtcgcga tttgctattt agggttatct ggttatgtgt ggtaccacga taataaacgc
    30961 agtaaacagg ccgatgttca ggcatctgct gtcagtgaaa ataataaggt tttaggcttt
    31021 ctccgcgaaa aaggatgcga ctattgccac acgccttcgg cagaattacc cgcctattat
    31081 tatattcctg gcgcgaaaca gttgatggat tacgacatta agcttggata taaatctttt
    31141 aacctcgagg ccgtgcgtgc ggcactgctg gctgataaac ccgtttcgca aagcgatctg
    31201 aataagattg aatgggtgat gcagtatgaa actatgccac caacgcgtta taccgcgcta
    31261 cactgggcgg gtaaggtgag tgatgaagag cgggcggaaa tactggcctg gattgcaaaa
    31321 cagcgcgcgg aatattacgc cagcaatgat actgctccgg agcatcgcaa tgaaccggtg
    31381 cagcccatcc cgcaaaaact gcctaccgat gcgcaaaaag tggcgttggg ctttgcgctg
    31441 tatcacgatc cccgtttatc ggctgatagc accatttcat gcgctcattg ccatgcgttg
    31501 aatgcggggg gcgtcgatgg cagaaaaaca tcgattggtg ttggtggcgc agttgggccg
    31561 attaacgcgc cgacggtatt taactcagta tttaacgttg agcagttctg ggatggtcgt
    31621 gcggcaacat tgcaggatca ggcaggtgga ccgccgttga acccgattga aatggcgtcg
    31681 aaatcctggg acgaaattat tgctaagctg gaaaaagatc cgcagcttaa agcgcagttc
    31741 ctcgaagttt atccgcaagg tttcagtggc gaaaatatta ctgatgccat tgctgaattt
    31801 gagaaaacat taattacgcc ggattcccca tttgataaat ggttgcgtgg agatgaaaat
    31861 gcgctgacgg cgcaacagaa aaaaggctat caattattta aagataataa atgtgcaact
    31921 tgtcatggtg gtattattct cggcggacgt tcctttgaac cgttggggct gaaaaaagac
    31981 tttaactttg gggaaattac ggcggcggat attggtcgta tgaatgtgac taaagaagag
    32041 cgtgataaat tgcgtcagaa agtacccggt ttacgtaacg ttgctttaac ggcaccgtac
    32101 ttccatcgcg gtgacgtgcc gacgctggac ggggcggtga aactgatgct gcgctatcag
    32161 gtaggcaaag agctgccgca ggaggatgtg gatgatatcg tagctttcct gcacagtctg
    32221 aacggggtgt acacgccgta tatgcaggat aaacaataat taatttgatc gcccgaacag
    32281 gaatgtttgg gcgattttta ttacg
//
LOCUS       DAZFKM010000046        31780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFKM010000046 DAZFKM010000000
VERSION     DAZFKM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..920)
                     /gene="rarD"
                     /locus_tag="ACHKAY_002209"
     CDS             complement(33..920)
                     /gene="rarD"
                     /locus_tag="ACHKAY_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO5700884.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(972..1439)
                     /gene="yigI"
                     /locus_tag="ACHKAY_002210"
     CDS             complement(972..1439)
                     /gene="yigI"
                     /locus_tag="ACHKAY_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO5700885.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1604..2473
                     /gene="pldA"
                     /locus_tag="ACHKAY_002211"
     CDS             1604..2473
                     /gene="pldA"
                     /locus_tag="ACHKAY_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO5700886.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2606..4435
                     /gene="recQ"
                     /locus_tag="ACHKAY_002212"
     CDS             2606..4435
                     /gene="recQ"
                     /locus_tag="ACHKAY_002212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO5700887.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4499..5119
                     /gene="rhtC"
                     /locus_tag="ACHKAY_002213"
     CDS             4499..5119
                     /gene="rhtC"
                     /locus_tag="ACHKAY_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO5700888.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5181..5801)
                     /gene="rhtB"
                     /locus_tag="ACHKAY_002214"
     CDS             complement(5181..5801)
                     /gene="rhtB"
                     /locus_tag="ACHKAY_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO5700889.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5912..6934
                     /gene="pldB"
                     /locus_tag="ACHKAY_002215"
     CDS             5912..6934
                     /gene="pldB"
                     /locus_tag="ACHKAY_002215"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO5700890.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6942..7742
                     /gene="yigL"
                     /locus_tag="ACHKAY_002216"
     CDS             6942..7742
                     /gene="yigL"
                     /locus_tag="ACHKAY_002216"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO5700891.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7818..8717
                     /gene="bioP"
                     /locus_tag="ACHKAY_002217"
     CDS             7818..8717
                     /gene="bioP"
                     /locus_tag="ACHKAY_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO5700892.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8605..9558)
                     /gene="metR"
                     /locus_tag="ACHKAY_002218"
     CDS             complement(8605..9558)
                     /gene="metR"
                     /locus_tag="ACHKAY_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO5700893.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9676..11937
                     /gene="metE"
                     /locus_tag="ACHKAY_002219"
     CDS             9676..11937
                     /gene="metE"
                     /locus_tag="ACHKAY_002219"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO5700894.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(11976..12791)
                     /gene="ysgA"
                     /locus_tag="ACHKAY_002220"
     CDS             complement(11976..12791)
                     /gene="ysgA"
                     /locus_tag="ACHKAY_002220"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO5700895.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13053..13814
                     /gene="udp"
                     /locus_tag="ACHKAY_002221"
     CDS             13053..13814
                     /gene="udp"
                     /locus_tag="ACHKAY_002221"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO5700896.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            13955..15382
                     /gene="rmuC"
                     /locus_tag="ACHKAY_002222"
     CDS             13955..15382
                     /gene="rmuC"
                     /locus_tag="ACHKAY_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO5700897.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15477..16232
                     /gene="ubiE"
                     /locus_tag="ACHKAY_002223"
     CDS             15477..16232
                     /gene="ubiE"
                     /locus_tag="ACHKAY_002223"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO5700898.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16246..16851
                     /gene="ubiJ"
                     /locus_tag="ACHKAY_002224"
     CDS             16246..16851
                     /gene="ubiJ"
                     /locus_tag="ACHKAY_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO5700899.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16848..18488
                     /gene="ubiB"
                     /locus_tag="ACHKAY_002225"
     CDS             16848..18488
                     /gene="ubiB"
                     /locus_tag="ACHKAY_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO5700900.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18567..18836
                     /gene="tatA"
                     /locus_tag="ACHKAY_002226"
     CDS             18567..18836
                     /gene="tatA"
                     /locus_tag="ACHKAY_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO5700901.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18840..19355
                     /gene="tatB"
                     /locus_tag="ACHKAY_002227"
     CDS             18840..19355
                     /gene="tatB"
                     /locus_tag="ACHKAY_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO5700902.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19358..20134
                     /gene="tatC"
                     /locus_tag="ACHKAY_002228"
     CDS             19358..20134
                     /gene="tatC"
                     /locus_tag="ACHKAY_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO5700903.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20176..20958
                     /gene="tatD"
                     /locus_tag="ACHKAY_002229"
     CDS             20176..20958
                     /gene="tatD"
                     /locus_tag="ACHKAY_002229"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO5700904.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20955..21443)
                     /gene="rfaH"
                     /locus_tag="ACHKAY_002230"
     CDS             complement(20955..21443)
                     /gene="rfaH"
                     /locus_tag="ACHKAY_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO5700905.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21610..23103
                     /gene="ubiD"
                     /locus_tag="ACHKAY_002231"
     CDS             21610..23103
                     /gene="ubiD"
                     /locus_tag="ACHKAY_002231"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO5700906.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23149..23850
                     /gene="fre"
                     /locus_tag="ACHKAY_002232"
     CDS             23149..23850
                     /gene="fre"
                     /locus_tag="ACHKAY_002232"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO5700907.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24042..25205)
                     /gene="fadA"
                     /locus_tag="ACHKAY_002233"
     CDS             complement(24042..25205)
                     /gene="fadA"
                     /locus_tag="ACHKAY_002233"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO5700908.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25215..27404)
                     /gene="fadB"
                     /locus_tag="ACHKAY_002234"
     CDS             complement(25215..27404)
                     /gene="fadB"
                     /locus_tag="ACHKAY_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO5700909.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27594..28925
                     /gene="pepQ"
                     /locus_tag="ACHKAY_002235"
     CDS             27594..28925
                     /gene="pepQ"
                     /locus_tag="ACHKAY_002235"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO5700910.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28925..29539
                     /gene="yigZ"
                     /locus_tag="ACHKAY_002236"
     CDS             28925..29539
                     /gene="yigZ"
                     /locus_tag="ACHKAY_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO5700911.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29578..31029
                     /gene="trkH"
                     /locus_tag="ACHKAY_002237"
     CDS             29578..31029
                     /gene="trkH"
                     /locus_tag="ACHKAY_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO5700912.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31041..31586
                     /gene="hemG"
                     /locus_tag="ACHKAY_002238"
     CDS             31041..31586
                     /gene="hemG"
                     /locus_tag="ACHKAY_002238"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO5700913.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7355 a         8124 c         8716 g         7585 t
ORIGIN      
        1 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
       61 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
      121 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
      181 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
      241 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      301 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
      361 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
      421 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
      481 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
      541 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      601 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      661 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
      721 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
      781 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcag gcacgtagta aatcaacttg
      841 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
      901 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
      961 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     1021 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     1081 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     1141 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1201 gtacttccca cgcacaccag acctgcggcg acatccagcg ctgacgcaat aaccccgccg
     1261 tgcaaaatgc tttgcgccca gttacccacc atcattggct gatttttaaa ggccagctgt
     1321 gcgaactctt tttcgtaacg ctccagctcc atccctaacg cgcggttaaa tggcatgtga
     1381 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     1441 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     1501 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     1561 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     1621 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1681 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     1741 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     1801 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     1861 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     1921 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     1981 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     2041 ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     2101 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     2161 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     2221 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2281 ggtgctcagt gcgaaaggac agtacaactg gaacaccggc tacggcggcg cggagttagg
     2341 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     2401 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtggggg ttatgctaaa
     2461 cgatttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     2521 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     2581 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     2641 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     2701 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     2761 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     2821 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     2881 ggcgtgcctt aactcgacgc aaacccgcga acagcaactt gaagtgatga caggctgccg
     2941 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     3001 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     3061 ccaatggggc cacgatttcc gcccggaata tgccgcgctc ggtcagttgc gccagcggtt
     3121 cccgacgctg ccgtttatgg cgctgaccgc cacagccgac gacaccacgc gccaggatat
     3181 cgtgcgcctg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     3241 tattcgctac atgctgatgg agaagttcaa accgctcgat cagttgatgc gctacgtgca
     3301 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     3361 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     3421 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     3481 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     3541 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     3601 gcccgcggaa gcgatgctgt tttacgatcc ggccgatatg gcgtggctgc gccgttgtct
     3661 ggaagagaag ccgcaggggc agttgcagga tatcgaacgt cataaactca atgcgatggg
     3721 cgcttttgcc gaagcgcaaa cttgccgtcg tctggtactg ctgaactatt ttggcgaagg
     3781 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     3841 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc gttttggtat
     3901 gggctatgtg gtggaagtga ttcgcggcgc taataaccag cgtatccgcg actatggtca
     3961 tgacaaacta aaagtctacg gtatgggccg tgataaaagt catgaacatt gggtgagcgt
     4021 gatccgccag ctgattcacc tcggtctggt gacgcaaaat attgcccagc attctgccct
     4081 acaactgaca gaggctgcgc gtccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     4141 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     4201 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg atagccgatg aaagtaatgt
     4261 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     4321 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     4381 caaaccgttt atggcgctga ttcgtgcgca tgttgatggc gatgacgaag agtagtcagc
     4441 agcataaaaa agtgccagta tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     4501 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     4561 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     4621 cgtgctgggc attacctgcg gcgtaatggt ttgggctggg attgcgctgc ttggcctgca
     4681 tttgattatc gaaaaaatgg cctggctgca tacgctgatt atggtgggcg gtggcctgta
     4741 tctctgctgg atgggttacc agatgctacg cggtgcactg aaaaaagagg cggtttctgc
     4801 acctgcgcca caggtcgagc tggcgaaaag tgggcgcagt ttcctgaaag gtttactgac
     4861 caatctcgct aatccgaaag cgattatcta ctttggctcg gtgttctcat tgtttgtcgg
     4921 tgataacgtt ggcactaccg cgcgctgggg catttttgcg ctgatcattg tcgaaacgct
     4981 ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg caaatgcgcc gtggttatca
     5041 acgtctggcg aagtggattg atggttttgc cggggcgtta tttgccggat ttggcattca
     5101 tttgattatt tcgcggtgat gccagacgcg tcttcagagt aagtcggata aggcgtttac
     5161 gccgcatccg acattatttt tcacgcatgc ctcgccgatg ctaacagcgc tcccaccagc
     5221 ataaacaacg agccgaaaat cttattcagc gccttcatct gctttggtcc tttaatccat
     5281 agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca caataatatc gaccacaata
     5341 gtggtgacgc cgagcacgat atactgcatc agttgcggct gttgcggcat gatgaattgc
     5401 ggaaatagcg ccgccagaaa cacaatactt ttgggattgg tgagattcac aaaaactgcg
     5461 cgctggaaca aatgtcgacg cgattgagta gaggccagcg atttaaggtc aattgcacca
     5521 gcggcgcgcc actgctggat tcccagccaa atcaagtaag ccgcgcctgc ccacttcaac
     5581 acttcaaacg caatcactga gcgggaaaat agcgtcccca accccacgcc aaccagcaca
     5641 atatgaatcg ccagtccggt ctgaagccca gcaatagacg ccaccgcgcc gcgataaccg
     5701 tggttgagcg aggtggtcat agtgttgatt gcaccagagc ctggcgacag gcttaaaatg
     5761 atcgatgtca gcaggtaggc aaaccaccat tctaaggtca tgatgaactc ccggtgtgtc
     5821 tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt caggcacgct aaaaaaaacg
     5881 attttacgtg gtttaagagg cagattaccc gatgtttcag cagcaaaaag actgggaaac
     5941 aagagaaaac gcgtttgctg cttttaccat gggaccgctg actgatttct ggcgtcagcg
     6001 tgatgaagca gagtttactg gtgtggatga cattccggtg cgctttgtcc gttttcgcgc
     6061 acagcaccat gaccgggtgg tagtcatctg cccggggcgt attgagagct acgtaaaata
     6121 tgcggaactg gcctatgacc tgttccattt ggggtttgat gtcttaatca tcgaccatcg
     6181 cgggcaggga cgttccggtc gcctgttagc cgatccgcat ctcgggcatg ttaatcgctt
     6241 taatgattat gttgatgatc tggcggcatt ctggcagcag gaggttcagc ccggtccgtg
     6301 gcgtaaacgc tatatactgg cacattcgat gggcggtgcg atctccacat tatttctgca
     6361 acgccatcca ggtgtatgtg acgccattgc gctaactgcg ccaatgtttg ggatcgtgat
     6421 tcgtatgccg tcatttatgg cacggcagat cctcaactgg gccgaagcgc atccacgttt
     6481 ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg ttgccgtttg ctatcaacgt
     6541 actgacccac agcagacagc gatatcgacg taacttacgc ttctatgctg atgacccaac
     6601 gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa agtattctgg ctggcgaaca
     6661 ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt ctcttacaag ctgaagagga
     6721 acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa ctccgcaccg ccgcgggcca
     6781 tcctgtcgaa ggaggacggc cgttggtaat taaaggtgct taccatgaga tcctttttga
     6841 aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt gattttttca acaggcataa
     6901 ctcacccagc ggaaaccgct ctacagaggt ttaaatttct tatgtaccag gttgttgcgt
     6961 ctgatttaga tggcacgtta ctttctcccg accatacgtt atccccttac gccaaagaaa
     7021 ctctgaagct gctcaccgcg cgcggcatca actttgtgtt tgcgaccggt cgtcaccacg
     7081 ttgatgtggg gcaaattcgc gataatctgg agattaagtc ttacatgatt acctccaatg
     7141 gtgcgcgcgt tcacgatctg gatggtaatc tgatttttgc tcataacctg gatcgcgaca
     7201 ttgccagcga tctgtttggc gtagtcaacg acaatccgga catcattact aacgtttatc
     7261 gcgacgacga atggtttatg aatcgccatc gcccggaaga gatgcgcttt tttaaagaag
     7321 cggtgttcaa atatgcgctg tatgagcctg gattactgga gccggaaggc gtcagcaaag
     7381 tgtttttcac ctgcgattcc catgaacaac tgctgccgct ggagcaggcg attaacgctc
     7441 gctggggcga tcgcgtcaac gtcagtttct ctaccttaac ctgtctggaa gtgatggcgg
     7501 gcggcgtgtc gaaaggccat gcgctggaag cagtggcgaa gaaactgggc tacagcctga
     7561 aggattgtat tgcgtttggt gacgggatga acgacgccga aatgctgtcg atggcgggga
     7621 aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga ccttcatccc gagctggaag
     7681 tgattggtac taatgccgac gacgcggtgc cgcattatct gcgtaaactc tatttatcgt
     7741 aatcgttctt tatttggtca gttgtcaacc tgatacttcg ctacaatgga tactcgttaa
     7801 tcaaagagtt ttccattgtg gcgctactta tcatcaccac gattctgtgg gccttctcct
     7861 ttagctttta tggcgagtac cttgcggggc acgtcgatag ctattttgcg gtgctggtgc
     7921 gcgttggcct ggcggcactc gtttttctgc cgtttctgcg tacccgtggc aatagcctga
     7981 aaacggtcgg cctgtatatg ttggtgggcg cgatgcagct cggcgtgatg tatatgctga
     8041 gtttccgcgc gtatctctac ctgacggtct ccgaactgct gctgttcacc gtactgacgc
     8101 cgctctacat cacgctgatt tatgacatca tgagtaagcg ccgtctgcgc tggggctatg
     8161 cctttagcgc cttgttggcg gtgattggtg ccgggattat tcgctatgat caggtcaccg
     8221 accatttctg gactggcttg ctgctggtgc aactctccaa tatcactttt gccatcggca
     8281 tggtgggtta caaacgcctg atggaaactc gcccgatgcc acagcataac gcctttgcgt
     8341 ggttctatct tggcgcgttt ctggtggcgg taattgcatg gttcttgctg ggaaatgcgc
     8401 agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt gtttcttggc gtggtggctt
     8461 ccgggattgg ctactttatg tggaactacg gcgcgacgca ggtggacgcc ggaacgctgg
     8521 gcattatgaa taatatgcac gttccggcag ggctgctggt aaacctggct atctggcacc
     8581 aacagccgca ctggccaacg ttcattacag gcgcgctggt gatcctggcc tcactgtggg
     8641 tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc agatgatcgc aggcgtgatt
     8701 gcgcgctgag cgaataaatg cttctgtaat cggctgacgc tgctcgccat cgcgcacggc
     8761 ggcgtacagt cggctccaca agccttcgcc cagggttttg gtcaccacca gaccctggcg
     8821 ctcaaaactc tctactaccc aatgcggtag cgcggcaata cccatccgcg cggcaaccat
     8881 ctgaatcaac aataaggtgt tatcgacgct tttcagtgac gggctgacgc ctgccggctg
     8941 aagaaaatgc cgccagacat ccagtcgact acgctgcacc ggataaatca acagcgtctc
     9001 gctggcgaga tcttccggtg taattcgcgt tttcgccgcc agtggatggt caggtgctaa
     9061 caccagacgc acttcatagt cgaacatcgg cgaataatgc aggccactgc gcggcaggat
     9121 atcggaggtc atcaccagat ccagctcgcc ctgttgcaac gcgggctgcg ggtcaaacgt
     9181 cacgcccgat ttaaaatcca tctctacctg cggccagttc ttatggaaat tttctaacgc
     9241 gggcgtcagc cactgaatac agctatggca ctcaatggca atgcgcagac gcgtctgctg
     9301 cggttcattg caggcttgca gtgcctggct aatttgcggc agcacctggt ttgccagttg
     9361 caacaggatt tctccctgcg gtgtaaagcg tagcggctgg ctcttacgca caaatagccg
     9421 gaagccaagg cgttgttcca gatcgctaaa ctggtgagac agggcggatt gcgtctgatg
     9481 caacgtcgcc gcagcggctg cgagagagcc gcagttccgc aacgcttgta gcgttttcag
     9541 gtgttttact tcgatcatga aagtccttca cttcggcatg aataatttgc gcttgaggaa
     9601 tatacagtaa ccgccaatta tggatgtgta aacatctgga cggctaaata ctaaaaattc
     9661 cataaggggc atataatgac aattcttaat cacaccctcg gtttccctcg cgttggcctg
     9721 cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac gcgtgaagaa
     9781 ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa gcaagcgggt
     9841 atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgttctgac caccagtctg
     9901 ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga tatcgacacc
     9961 ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc agcggaaatg
    10021 accaaatggt ttaacaccaa ctatcactac atggtgccgg aattcgttaa aggccaacag
    10081 ttcaaactga cctggacgca gctgctggag gaagtggacg aggcgctggc gctgggccac
    10141 aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa agtgaaaggt
    10201 gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca gcaagtgctg
    10261 gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc gctggtactg
    10321 gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct ccagggacag
    10381 gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga cacgattact
    10441 gcgctgcctg ttcagggtct gcatgttgac ctcgtacatg gtaaagatga cgttgctgaa
    10501 ctgcacaagc gcctgccttc tgactggttg ctgtctgcgg gtctgatcaa tggtcgtaac
    10561 gtctggcgcg ccgatcttac cgagaaatat gcgcaaatta aggacattgt cggcaaacgt
    10621 gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct gagcgtggaa
    10681 acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa atgccatgaa
    10741 ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc agagtggagc
    10801 gccccgattc aggcacgtcg tcactctacc cgcgtacata atccggcggt agaaaagcgt
    10861 ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt gcgtgccgaa
    10921 gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg ttctttcccg
    10981 caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct cgacgccaat
    11041 aactaccgca cgggcattgc ggaacatatc aggcaggcca ttgttgaaca ggaacgtttg
    11101 ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga atactttggc
    11161 gagcacctcg acgggtttgt ctttacgcaa aacggttggg tacagagcta cggttcccgc
    11221 tgcgtgaagc caccgattgt tattggtgac gttagtcgcc cggcaccgat taccgtggag
    11281 tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct gacggggccg
    11341 gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac catcgccaaa
    11401 cagattgcgc tggcgctgcg tgatgaggtg gccgatctgg aagcggcagg gattggtatc
    11461 atccagattg acgaaccggc gctgcgcgaa ggtttaccac tgcgtcgtag cgactgggac
    11521 gcctatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc gaaagatgac
    11581 acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga ttccattgcg
    11641 gcgctggatg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga gttgctggag
    11701 tcgtttgaag agtttgatta tccgaatgaa atcggtccag gcgtctatga cattcactcg
    11761 ccaaacgtac cgagcgtgga atggattgaa gccctgctga agaaagcggc aaaacgcatt
    11821 ccggcagagc gcctgtgggt caacccagac tgtggcctga aaacgcgcgg ctggccagaa
    11881 acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtct ggggtaaatc
    11941 caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt cccaccatac
    12001 tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc atgatagctc
    12061 gggcgataat cagcgttgaa tgcatgcccg gcatccgggt agacgataat ctctgctttc
    12121 gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg aatgctgtta
    12181 tcctgaccgc catataagcc gagaaccggc gcgttaagat cggttgcgat atcaacaggt
    12241 tgtttcggtg aattcagcga cttatcgccc gtcagtttgc cgtaccacgc cactgcggct
    12301 tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca gcagaatccg
    12361 gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc gacatgatcg
    12421 agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag caacgtgggg
    12481 atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc gatagccaga
    12541 tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt
    12601 tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct ggcatggtaa
    12661 gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc
    12721 ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc agattgttgt
    12781 gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc
    12841 agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga
    12901 agagtgattt atgtgatttg catcactttt ggtgggtaaa tttatgcaac gcatttgcgt
    12961 catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct ttttgcttct
    13021 tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt tttcatctcg
    13081 gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc gacccggatc
    13141 gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct caccgcgaat
    13201 tcaccacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct accggtatcg
    13261 gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt cgcaccttcc
    13321 tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat gtcctggtta
    13381 ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg gaattcccgg
    13441 ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatcc attggcgcga
    13501 caactcacgt tggcgtgaca gcttcttctg ataccttcta cccaggtcag gaacgttacg
    13561 atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag tggcaggcga
    13621 tgggcgtaat gaactatgaa atggaatctg caaccctgct gaccatgtgt gcaagtcagg
    13681 gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa gagatcccga
    13741 atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg gaagcggcgc
    13801 gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc ttttgtattt
    13861 ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc acaattctat
    13921 tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca tggtttacgc
    13981 agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc aacatgcgca
    14041 gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa
    14101 acaacaaatt acccaaagcg agcactggcg tgcagagtgc gagttactca ataacgaagt
    14161 gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag taaccacgcg
    14221 gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta acagcgagca
    14281 gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg
    14341 ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac gtgaacaact
    14401 ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag aacgccatac
    14461 cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc aggaagcgat
    14521 caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg gcgaggtagt
    14581 attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg aaacccaggt
    14641 cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc ggctgccgca
    14701 gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac gctattttaa
    14761 cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa
    14821 ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc gaactctgga
    14881 ttacgtgctg atgtttattc ccgttgaacc cgctttttca ctggcgcttg accgccagcc
    14941 ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga ctacgctgct
    15001 ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc gcaacgccca
    15061 gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca tcgatgacat
    15121 gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg caatgaaaaa
    15181 actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt
    15241 agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc aggatgaaga
    15301 gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac gcgatgatga
    15361 atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga aatctagggc
    15421 atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc attgagatgg
    15481 tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag gaacaaaaag
    15541 cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc atgaatgatt
    15601 tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc agcggcgtac
    15661 gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca gcgaaattct
    15721 cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa tccatgctca
    15781 aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag tatgttcagg
    15841 cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc atttcgtttg
    15901 gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc gtgctgaaac
    15961 ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg ctgagcaaag
    16021 cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg
    16081 ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag gataccctga
    16141 aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg acggcggggg
    16201 ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc ttttaaacct
    16261 ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc acccgcgctg
    16321 aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa aggcttttcg
    16381 acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga atgggcaggc
    16441 gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag
    16501 cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat tcaggtggtg
    16561 caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact gctggcccct
    16621 tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc
    16681 ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac tgaagagtgg
    16741 cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag
    16801 cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg acgccaggtg
    16861 aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt gatgaactga
    16921 tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta ttctggatgc
    16981 caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg caagaactgg
    17041 ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt tttccaccgc
    17101 atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat ggcaagctgg
    17161 cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt
    17221 ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg cgattgaaat
    17281 cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg gtcattaaag
    17341 cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc
    17401 gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt gatgaactga
    17461 atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa gacagcccga
    17521 tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg gtgatggagc
    17581 gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc actaacatga
    17641 aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc gacagctttt
    17701 tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg gaaaacccga
    17761 aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat aaacgctatc
    17821 tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca gagctacacg
    17881 tcgattcagg ctgggtgcca ccagatacca acgttgaaga gttcgaattt gccattcgta
    17941 cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga catgtactgt
    18001 taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa ctggtgttac
    18061 tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg cagctcgatt
    18121 tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc ggtattcctg
    18181 cgctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc
    18241 ctgaattggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt gttgataaga
    18301 ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat tttctcggaa
    18361 ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct gagtgggggc
    18421 tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa
    18481 cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg ctttgtttaa
    18541 tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag ttattgatta
    18601 ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc ggttccgatc
    18661 ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca aagcaggata
    18721 aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag gcggatacga
    18781 atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag gtgtaatccg
    18841 tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc gtcgttctgg
    18901 ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt
    18961 cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag gagtttcagg
    19021 acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa ctgaaagcgt
    19081 cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc
    19141 ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa gataatgaag
    19201 ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg ccggaacaga
    19261 agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg
    19321 ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag atactcaacc
    19381 gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat
    19441 cgtgatattc ctgtgtctgg tctatttcgc caatgatatc tatcacctgg tatccgcgcc
    19501 attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt
    19561 ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc cggtgattct
    19621 ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc gcctggtggt
    19681 gccgctgctg gtttccagct ctctgctgtt ttatatcggt atggcattcg cctactttgt
    19741 ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg tgcaggtatc
    19801 caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt ttggtgtctc
    19861 ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct cgccagaaga
    19921 cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga tgttgctgac
    19981 gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc tgtttgaaat
    20041 cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag aggaaaacga
    20101 cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca gggcggttgt
    20161 catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca atttgcgaaa
    20221 gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct actcatcacc
    20281 ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt
    20341 tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc gactgaagaa
    20401 gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg tggtctcgac
    20461 tttaaccgca acttttcgac gccggaagag caggaacgcg cttttgttgc tcagctacgc
    20521 attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca cgagcggttt
    20581 atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca ttgctttacc
    20641 ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg cattaccggt
    20701 tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt gattccggcg
    20761 gagaaattgc tgatcgaaac tgatgcgccg tatctgctcc cgcgcgatct cacgccaaag
    20821 ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg tattgcgcac
    20881 tggcgtggag aagatgccgc atggctggct gccaccacgg atgctaatgt caaaacactg
    20941 tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg cttaatctct
    21001 ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc ggtgaaaatg
    21061 gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg ataaggggtt
    21121 gccggatcga caatgtcttt cggtttatat accgatagct gatgaatcac cgccgatggg
    21181 actatcgctg gcgacgcgcc aaagcgcacg aagtggctga caccgcgggt cgcgttgata
    21241 gtcgtggtat gaatcacttc tggatcaaat tcgacaaaca ggtagttggg aaacaatggc
    21301 tcactgactg cagtacgttt tccacgcacg attttttcca gggtgatcat cggtgccagg
    21361 caattcacag cctgtctttc gaggtgttcc tgggcacgtt gaagttgccc gcgcttgcag
    21421 tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc gcaaggatag
    21481 caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt cagagttcac
    21541 gctaatttaa caaatttaca gcatcgcaaa gatgaacgcc gtataatggg cgtagattaa
    21601 gaggctacaa tggacgccat gaaatataac gatttacgcg acttcttgac gctgcttgaa
    21661 cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga aatcactgaa
    21721 attgctgacc gcactttgcg tgccggtggg cctgcgctgt tgttcgaaaa ccctaaaggc
    21781 tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc gatgggcatg
    21841 gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag
    21901 ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt taagcaagta
    21961 ttgaacatgc cgacaaagcg gctgcgtggt gcgccctgcc aacaaaaaat cgtctctggc
    22021 gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga tgccgcgccg
    22081 ctgattacct gggggctgac agtgacgcgc ggcccgcata aagagcggca gaatctgggc
    22141 atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc
    22201 ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg tttcccggtt
    22261 tctgtggcgc tgggtgccga tcccgccacg attctcggtg cagtcacccc cgttccggat
    22321 acactttcag agtatgcgtt tgccggattg ctgcgcggta ccaagaccga agtggtgaag
    22381 tgtatctcca atgaccttga agtgcccgcc agtgcggaga ttgtgctgga agggtatatc
    22441 gaacaaggtg aaacggcgcc ggaagggccg tatggcgacc acaccggtta ctataacgaa
    22501 gtcgatagtt tcccggtatt taccgtgacg catattaccc agcgtgaaga tgcgatttac
    22561 cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt ggcgttgaac
    22621 gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg
    22681 ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta cgccggacac
    22741 gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta cactaaattt
    22801 gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat ttgggcgatt
    22861 accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc tattgattat
    22921 ctggattttg cctcgcctgt ctccgggctg ggttcaaaaa tggggctgga tgccacgaat
    22981 aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt
    23041 gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc
    23101 tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat gacaacctta
    23161 agctgtaaag tgacctcggt agaagctatc acggataccg tatatcgtgt ccgcatcgtg
    23221 ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat ggatgagcgc
    23281 gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat cgagctgcat
    23341 attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat cctcaaagat
    23401 catcaaatcg tggtcgatat tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt
    23461 ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat tttgctgaca
    23521 gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg tgaagagcag
    23581 catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccctgg tctgcaagtg
    23641 gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac cgtgttaacg
    23701 gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc cggacgtttt
    23761 gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg ggaagatcgc
    23821 ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc tgacaggcgg
    23881 gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca tccggcaatc
    23941 ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg gtgcaggatc
    24001 ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc
    24061 gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg aacgtctttg
    24121 cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacagcc cagcggatga
    24181 cccagcgcga tcgcaccacc gttgaggttg atcttctcgt caatctgctc aatcagtccc
    24241 agatctttaa tacatggcag gatctgcgcg gcaaaggctt cgttcatctc aaacacgccg
    24301 atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg aaccgggccg
    24361 taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg agcgcgcggc
    24421 ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg
    24481 gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa cgccggacgc
    24541 agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc
    24601 ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt aaatgcggcc
    24661 gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca
    24721 cggctgatac cgtgcatacg cgccagcatt tctgctgtta agcccatcat gcccgccgct
    24781 ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat cggcacatgg
    24841 cccatatgct ccacgccgcc aaccagacat gcctgcgcat cgccggtcat aatcatccgt
    24901 gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt aaccgccggg
    24961 acagagtgtg gtacttctgc cagcagcgcc gcgttacggg cgatgttaaa accctgctcc
    25021 agcgtctgct gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg
    25081 ttacgcgcca gcaggctacg cattaaatga gcggagagat cttctgcacg cacgttacga
    25141 aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt
    25201 tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccgga
    25261 ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcatacagc
    25321 gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc gagggtgtcc
    25381 agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac cagcgccata
    25441 tccgcttccg ccggagtggc gataatgcct tcctccagac agcgcaccac ttcgttgacc
    25501 atcgggatca tcatgcgggc gataatctcc tcttcgctga aatcgcgctt cggctggctc
    25561 acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt acctttgctg
    25621 tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt ggcatcaaac
    25681 agcgcgtcga tggcatcgcg gtaatctttc tgcatcctct gcgggaagcc tgctgccatg
    25741 acagcctggg catgatgcgc ggtgtcaatg cccacgacat ccagcagata tgccggaccc
    25801 atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg
    25861 cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac aaagaagccg
    25921 gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc gacaactttc
    25981 gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg cattcggtgg
    26041 accgggttaa agaagtgcat cccgcagaag ttttccgggc gtttcagcgc gttggccagt
    26101 tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg tactttttgt
    26161 tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc ttctaccaca
    26221 atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggattgtgga gatcacgcca
    26281 gccagtttca gaccatcgat cttgccgcgc tcaagctgct tgttcagcag tttcgcggct
    26341 tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac cggcacgcct
    26401 ttccacgcag actggtaagc gatgccgccg cccataatgc ctgcacccag caccgcggcc
    26461 tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac atattgatcg
    26521 ttaaggaaaa tgccgaccag tgcgcgggct tcgttggtat gcgccagcgg gacaaaactt
    26581 ttgttttcca ggtttaaggc ttcttcacga ccaaaacggg ccgcagcttc aatggttttt
    26641 actgcggtga tgggggccgg ataatgtttc cccgctgttt gtgcgaccat ccctttagcg
    26701 atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gtggttccag cttcggctga
    26761 cgttttgctt tccagtcgag gtcgccgtta atggcctggc gtaaaaccgc ctttgcgcct
    26821 tcaaccagtt tttctgcttt gactacgcca tccaccagac cgattttcag cgcctgatcc
    26881 gcgccgacat ctttaccggc ggcaatgatt tccagcgcac tgtctgcgcc cagcatacgt
    26941 ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc cggcagaccg
    27001 atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc gcagccaccg
    27061 cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc caggcgatta
    27121 aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac gaggaacagg
    27181 gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga acgcagcagc
    27241 agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag gctggcgacg
    27301 gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc ggcaatgcca
    27361 tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc
    27421 cgcaaggtga tctggtcgta ccagatgcat cgaagtgtgt attttgtgtt aaaaatatgc
    27481 aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac gctctggatg
    27541 aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa aaaatggaat
    27601 cactggcctc actctataaa aatcatatag ctaccttaca ggaacggact cgcgatgcgc
    27661 tggcgcgctt caagctggat gcgttactta ttcactccgg cgaactgttc aacgtttttc
    27721 tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg gtgccggtaa
    27781 ctcaggtgcc aaactgctgg ctgctggtgg atggcgtgaa caagccgaaa ctgtggttct
    27841 atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctctttc tggactgaag
    27901 acgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg cctgctgcgc
    27961 gcgggaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg ggtattgagg
    28021 ccagcaatat caacccgaaa ggggtgatcg actacctgca ttactaccgc tccttcaaaa
    28081 ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaac ggtcatcgcg
    28141 cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaacatt gcctatctga
    28201 ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg ctcaacgaac
    28261 acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag atgcgcagct
    28321 tcctgctgga tgccggggcc gaatataacg gctatgccgc tgacctgacc cgtacctggt
    28381 cggcaaaaag tgacaacgat tacgcacagc tggtgaaaga cgtaaatgat gaacaactgg
    28441 cgctgatcgc gaccatgaaa gctggcgtta gctatgtgga ttaccacatc cagttccatc
    28501 agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt gaagaagcga
    28561 tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tatcggccat ccgctgggcc
    28621 tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacgcac ctcgcggcac
    28681 cggcaaaata tccgtacctg cgctgcaccc gtattctcca gccgggcatg gtgttaacca
    28741 tcgaaccggg tatctacttc attgaatcgc tactggcacc gtggcgtgaa gggcagttca
    28801 gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc attcgtatcg
    28861 aagacaacgt ggtgatccac gaaaataacg tggaaaacat gacccgggat ctgaaactgg
    28921 cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag agatcaaaaa
    28981 gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt
    29041 tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt gggtggcggg
    29101 tgcgccggat gattcacaac agctgggttt ctctgacgac ggggagccgg cgggaacggc
    29161 aggtaaaccg atgctcgccc agctaatggg cagcggcgtt ggggaaatta ccgctgtggt
    29221 ggtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag cgtatggcgg
    29281 cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat taaccgaata
    29341 tactttgcaa tgtgaatatc atcagttaac cggcattgaa gcgttgctgg ggcagtgtga
    29401 cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg cgcttccggc
    29461 ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt cattgcaatt
    29521 gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg
    29581 cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc agggaccatg
    29641 attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc ttttacccag
    29701 accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg caaagagaaa
    29761 ggcgaactta aatcccgtga ggggtttctg atagtggtgc tgttctggac cgtgctgggt
    29821 agcgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat taccgatgcg
    29881 ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt ggggctggat
    29941 tcgctccctc acgccatcct cttttatcgc cagatgctgc aatggtttgg cgggatgggg
    30001 atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat gcagctctat
    30061 cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat tgcggaaacg
    30121 gcgaaaaccc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg
    30181 tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat cgctattggc
    30241 ggcttctcga cacatgatgc cagtatcggt tatttcgata gcccgactat taacactatc
    30301 attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc actgttaagt
    30361 gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat cggcgtacag
    30421 tttacgctgg tggttatttg taccctcgta ctgtggtttc ataatgtcta cagttcggcg
    30481 ctgatgacaa ttaaccaggc gtttttccag gtggtgtcga tggcgacaac cgccgggttt
    30541 acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt atgttcggct
    30601 tttatcggcg gttgtgccgg gtcaacgggc ggtggcctga aagtgatccg catcctgctg
    30661 ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc cgtctatagc
    30721 attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg gggatttttc
    30781 tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac gggcgtggat
    30841 gacttttctg cctttgcctc agttgttgcg acattgaata acctggggcc agggcttggc
    30901 gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct gattgccaac
    30961 atgctgtttg gtcgtctcga ggtctttaca ttgctggtgc tctttacccc gactttctgg
    31021 cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg gacggacaaa
    31081 cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc cagacggatg
    31141 tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ttatgaccgt gtggtcattg
    31201 gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc aaaaaacatg
    31261 cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg gcgcgcaaac
    31321 cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg aactcgcaat
    31381 ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt
    31441 acgaccgttt tatgatcaag ctgattatga agatgtcagg cggtgaaacg gatacgcgca
    31501 aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa atcgcccatt
    31561 taaccgacaa accgacgctg aaataagcac aaagaataaa aaatgcgcgg tcagaaaatt
    31621 attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg
    31681 gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaattcc gacagagaaa
    31741 gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt
//
LOCUS       DAZFKM010000047        30980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFKM010000047 DAZFKM010000000
VERSION     DAZFKM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(283..1873)
                     /gene="lsrK"
                     /locus_tag="ACHKAY_002239"
                     /pseudo
     CDS             complement(283..1873)
                     /gene="lsrK"
                     /locus_tag="ACHKAY_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1952..2905)
                     /gene="lsrR"
                     /locus_tag="ACHKAY_002240"
     CDS             complement(1952..2905)
                     /gene="lsrR"
                     /locus_tag="ACHKAY_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO5700914.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3154..4689
                     /gene="lsrA"
                     /locus_tag="ACHKAY_002241"
     CDS             3154..4689
                     /gene="lsrA"
                     /locus_tag="ACHKAY_002241"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO5700915.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4683..5711
                     /gene="lsrC"
                     /locus_tag="ACHKAY_002242"
     CDS             4683..5711
                     /gene="lsrC"
                     /locus_tag="ACHKAY_002242"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO5700916.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5711..6703
                     /gene="lsrD"
                     /locus_tag="ACHKAY_002243"
     CDS             5711..6703
                     /gene="lsrD"
                     /locus_tag="ACHKAY_002243"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO5700917.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6715..7737
                     /gene="lsrB"
                     /locus_tag="ACHKAY_002244"
     CDS             6715..7737
                     /gene="lsrB"
                     /locus_tag="ACHKAY_002244"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO5700918.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7764..8600
                     /gene="lsrF"
                     /locus_tag="ACHKAY_002245"
                     /pseudo
     CDS             7764..8600
                     /gene="lsrF"
                     /locus_tag="ACHKAY_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8599..9093
                     /locus_tag="ACHKAY_002246"
                     /pseudo
     CDS             8599..9093
                     /locus_tag="ACHKAY_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="catalyzes the formation of
                     (E)-3-(methoxycarbonyl)pent-2-enedioate and
                     S-adenosyl-L-homocysteine from S-adenosyl-L-methionine and
                     trans-aconitate; internal stop; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
     gene            complement(9097..10011)
                     /gene="yneE"
                     /locus_tag="ACHKAY_002247"
     CDS             complement(9097..10011)
                     /gene="yneE"
                     /locus_tag="ACHKAY_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO5700919.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10218..11669)
                     /gene="uxaB"
                     /locus_tag="ACHKAY_002248"
     CDS             complement(10218..11669)
                     /gene="uxaB"
                     /locus_tag="ACHKAY_002248"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO5700920.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11896..13314)
                     /locus_tag="ACHKAY_002249"
     CDS             complement(11896..13314)
                     /locus_tag="ACHKAY_002249"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO5700921.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13453..13812)
                     /gene="yneG"
                     /locus_tag="ACHKAY_002250"
     CDS             complement(13453..13812)
                     /gene="yneG"
                     /locus_tag="ACHKAY_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO5700922.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13812..14738)
                     /gene="glsB"
                     /locus_tag="ACHKAY_002251"
     CDS             complement(13812..14738)
                     /gene="glsB"
                     /locus_tag="ACHKAY_002251"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO5700923.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14802..16190)
                     /gene="sad"
                     /locus_tag="ACHKAY_002252"
     CDS             complement(14802..16190)
                     /gene="sad"
                     /locus_tag="ACHKAY_002252"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO5700924.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16291..17172
                     /gene="yneJ"
                     /locus_tag="ACHKAY_002253"
     CDS             16291..17172
                     /gene="yneJ"
                     /locus_tag="ACHKAY_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5700925.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17250..18364
                     /gene="yneK"
                     /locus_tag="ACHKAY_002254"
                     /pseudo
     CDS             17250..18364
                     /gene="yneK"
                     /locus_tag="ACHKAY_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18514..19704
                     /gene="ydeA"
                     /locus_tag="ACHKAY_002255"
     CDS             18514..19704
                     /gene="ydeA"
                     /locus_tag="ACHKAY_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO5700926.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(19729..20394)
                     /gene="marC"
                     /locus_tag="ACHKAY_002256"
     CDS             complement(19729..20394)
                     /gene="marC"
                     /locus_tag="ACHKAY_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO5700927.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            20606..21040
                     /gene="marR"
                     /locus_tag="ACHKAY_002257"
     CDS             20606..21040
                     /gene="marR"
                     /locus_tag="ACHKAY_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO5700928.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21060..21443
                     /gene="marA"
                     /locus_tag="ACHKAY_002258"
     CDS             21060..21443
                     /gene="marA"
                     /locus_tag="ACHKAY_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO5700929.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21475..21693
                     /gene="marB"
                     /locus_tag="ACHKAY_002259"
     CDS             21475..21693
                     /gene="marB"
                     /locus_tag="ACHKAY_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO5700930.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(21750..23189)
                     /locus_tag="ACHKAY_002260"
     CDS             complement(21750..23189)
                     /locus_tag="ACHKAY_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO5700931.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23214..24887)
                     /locus_tag="ACHKAY_002261"
     CDS             complement(23214..24887)
                     /locus_tag="ACHKAY_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO5700932.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(24943..25254)
                     /locus_tag="ACHKAY_002262"
     CDS             complement(24943..25254)
                     /locus_tag="ACHKAY_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO5700933.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25282..26604)
                     /locus_tag="ACHKAY_002263"
     CDS             complement(25282..26604)
                     /locus_tag="ACHKAY_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO5700934.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(26719..27030)
                     /locus_tag="ACHKAY_002264"
     CDS             complement(26719..27030)
                     /locus_tag="ACHKAY_002264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO5700935.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27229..27927
                     /locus_tag="ACHKAY_002265"
     CDS             27229..27927
                     /locus_tag="ACHKAY_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5700936.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(27972..28871)
                     /gene="eamA"
                     /locus_tag="ACHKAY_002266"
     CDS             complement(27972..28871)
                     /gene="eamA"
                     /locus_tag="ACHKAY_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO5700937.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29066..30253
                     /gene="ydeE"
                     /locus_tag="ACHKAY_002267"
     CDS             29066..30253
                     /gene="ydeE"
                     /locus_tag="ACHKAY_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO5700938.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            30380..30475
                     /gene="mgtS"
                     /locus_tag="ACHKAY_002268"
     CDS             30380..30475
                     /gene="mgtS"
                     /locus_tag="ACHKAY_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO5700939.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            30475..30579
                     /gene="mgtT"
                     /locus_tag="ACHKAY_002269"
     CDS             30475..30579
                     /gene="mgtT"
                     /locus_tag="ACHKAY_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO5700940.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(30694..>30980)
                     /gene="dgcZ"
                     /locus_tag="ACHKAY_002270"
     CDS             complement(30694..>30980)
                     /gene="dgcZ"
                     /locus_tag="ACHKAY_002270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5700941.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         8086 a         7678 c         7196 g         8020 t
ORIGIN      
        1 atcatcatca tatttaacaa agaatagcac taattgctaa aaatcgaagt ttattaaacc
       61 ccttttgcat ccattaatgt ctttaggaat attcgctata aaataagggt tatgcttaat
      121 gctttaagaa aaatagcaat tttcccttga atatagtact ggtgatggaa cgatgaatct
      181 gcaagctggc atttaacaag ccagctctaa aagaagggaa ataagaaaaa ctatactcaa
      241 aaactaacag ccacggtcat catgatgtgg ccgtcaatga aactataacc caggcgcttt
      301 ccataacgac gtcgtcagtc catgatcaac cagccccagc tgatcctgat aaactgcctg
      361 ccacttatcg cgtgaatcct gataaagttc atgcttttcc gggtctggtg tgtgcgtccg
      421 ttcccagcga accaggcgtt ctccggtttc tgccattgat gaaaaaattc cggcaccgac
      481 gccagctgca atggcacatc ctaatgcggt ggcttctttg actaccggaa tattgacggg
      541 taatcccgag acatcagcga gaatttgact ccataatttc ccttttgaac ctccgccagc
      601 aaaaaccagc gaggtgggat gaatattcga gaaatcagca atttgctgca agttacacgc
      661 tgatacaatc gccgcatttt cttccagcgc acggaacaat gtcgctttgt tacatttatc
      721 cgggtcaatg gacaagttaa taaaggaagg cgcagcgtga taccaggttt taaagcgcat
      781 tctgtcggag aagatcggca ttacgcccca cgacccaggc ggcacccgac tggccatctc
      841 ttccagcagc gtataggtgt cgatgcctaa acgttcggca atcagttttt cttcggcaca
      901 gaaagcatcg cggaaccagc gcatggtgag tccggtaaaa aagcttatag attcagcttg
      961 taccatgcca ggaataacat gaggattaac gcgcacgttc atttctgggt ctgtcaccgg
     1021 cgcggctaaa tttacaactt gctgccagaa tgtgccgcca agaaccgcgg tttgtgccgg
     1081 acgcacaacg cctaacccaa ggcaaccaag ctgcacgtcg cctcctccaa cgaccaccgg
     1141 agtgcccgcc ttcagaccgc agagttccgc cgcttgtgaa cttaccacgc ccagcaatgt
     1201 gccggtttct ttgacaggag aaagaatatc ggcacgcagg ccagccatat ccagcaatgc
     1261 aggtttccag tcacgggtgg ttagatcaaa gtcccgtggt gccagcgtta gagggatcca
     1321 ccgccagttc gccgctgagc atataggcca gccagtcgct gatcatggtg atggttgatg
     1381 cctgacggta aatatcggaa cgatggtgcg ccagccaaag taatctgggg atggcactta
     1441 aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc aaaggtattg ttgtgcagtt
     1501 ctttaagttc gctaacttcg cgcgctgctc tggcatccac attggcgcag gcccagatcg
     1561 gtgttccttc attgttatat aaaacaatgc cttcacgcat agaacatgcc gaaacggcag
     1621 cgatatactc cgggtctatg ccggcgttgt gcagcgcctg gcgcatacac tcacacgcca
     1681 gttgccagtt tttgttgaga tcaaattcca tagaaccagg aacgtccggt actgccagat
     1741 gccgccactc cgcctgtccc actgctattt gattgccttt caggtcgaaa atcacagccc
     1801 gaatacttcc ggtgcctgca tccagcgcca acaggtaata ctttgattct gaaggggtaa
     1861 agagtcgagc cattagatta tcctcggctt atgcgttctg tctgttcctc tatacgttct
     1921 ccatcattcc cggtaataag gtcatgcaaa tttaactacg taaaatcgcc gctgctgtat
     1981 cctgatcggt aaccagtgcg ttgatataac cgcctttcat tgcagcggca attgcttcgg
     2041 ctttattttc tccccctgcc acgccaaccc ggacgggtat ggtcttcagc gcgcttaaag
     2101 gtaagccaat cagttcgtta tgtattttaa tatccgtgac aacgtcacct tttgcatcaa
     2161 aaaagtagcc taaaatgtcg ccaaccgccc cttttcgacc aatcattaac tgttcgccct
     2221 ggctgatata accggagcga atgatcgtcg cgtcgtcctg ttgactgacg gccccaatgc
     2281 cgacaatcgc cacatccgct gcttgcgcgg ctaacagaac atctttgacg caattttcat
     2341 tttttagcgt acgggcaatg tcagcggagg atgcccgcaa cggagccgga ataatattca
     2401 cactgcacgc cgcgttaagc tgcccgatac ccgtcatata agaaccgacg ccaccggaga
     2461 gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact taagcgttgc agcgtattca
     2521 tggttgcctc gccaaaacca atcgccagca tctgttgtgg ttgaagtaaa ctcatcaaca
     2581 tatgcgccgc gcctatccct agtcgcccgc cgacatcagc atccgcaagc ccagggatca
     2641 cccggacatg ttgcagcgaa aactgacgac gtaattgagt ttcatattcc agacagcctt
     2701 caaagcgaga attaatctgt acgcgaataa tgccggactg atgccctttc tccagcaagc
     2761 gcgacacttt caaacgggtc aggccgagac gatcgctgat ctcgctctgg gtcagcccgt
     2821 cgtgatagta aaaccacgcg atccgcgcga cctgttcttc ttcacacatt ccctgttctg
     2881 aaattgccga atcgttgatt gtcataattc attcttcact ttgaacatat ttaaatcttt
     2941 aatgcaattg ttcagttctt gcgcatttat atctgtgatg gcagccacag tttgactcta
     3001 cgagcatgaa caaacgcaac cgtgaaaatc aaaatagcat aaattgtgat ctattcgtcg
     3061 gaaatatgtg caatgtccac ctaaggttat gaacaaatta aaagcagaaa tacatttgtt
     3121 caaaactcac ctgcaaaact gaacggggga aatatgcaaa cgagtgatac ccgcgcgtta
     3181 ccgctacttt gcgcccgctc ggtttataaa cagtattcag gggtcaatgt cctgaaaggc
     3241 atcgatttta cgttgcatca gggggaggtc cacgccctgc tcggcggcaa tggtgccggt
     3301 aaatcgacgt taatgaagat tattgccggt attacccctg ctgatagcgg tacgctggag
     3361 attgggggca acaactacgc cagattaacg ccagttcatg ctcatcagct gggcatttat
     3421 ctcgttcccc aggaaccgct gcttttccca agcctgtcga taaaagaaaa catcctgttt
     3481 gggatggcaa aaaaacagct ctccatgcaa aaaatgaaga acttgctggc ggcgctgggc
     3541 tgccagtttg atctgcatag tctggcagga tcgctggatg tcgccgatcg ccaaatggtg
     3601 gaaatcctcc gcgggctaat gcgcgactcg cggattctga tcctcgatga acctaccgcc
     3661 tcgcttaccc ctgcggaaac cgaacgcttg tttactcgct tgcaagagct gcttgctact
     3721 ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa ttcgccagat tgccgatcga
     3781 attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga actgtctacc
     3841 gacgacatta ttcaggccat cacgccagtg gtacgggaaa aatcgctctc tgccagccaa
     3901 aaattatggc tggagttacc tggtaaccgc ccacaacatg ctgccggaac gccggtgctg
     3961 acactggaaa atctgaccgg cgaaggtttc aggaatgtca gcatgacgct caatgccgga
     4021 gaaattctgg gcctggctgg gctggtgggg gccggacgca cagaactggc cgagacgttc
     4081 tatggtctgc gtactttgcg tagcggacgc attatgctga atggtaaaga gatcaataaa
     4141 ttatccactg gagaacgttt actgcgcggt ctggtttatc tgccggaaga tcgccagtca
     4201 tccggactga atctcgatgc ttcgctggcc tggaacgtct gcgcccttac tcataacctt
     4261 cgtggattct gggcgaaaac cgcgaaagat aatgccaccc tggaacgtta tcgtcgggcg
     4321 ctgaatatta aatttaacca accggaacaa gctgcacgga cattatccgg tggcaaccag
     4381 caaaaaatcc tcattgccaa atgcctggaa gcttcgccgc aagcattgat tgtcgatgag
     4441 ccgacgcgcg gcgtggatgt ctcggcccgt aatgatatct accagctgtt gcgcagcatc
     4501 tccgcacaga atgtggctgt gctgcttatc tcctctgatc tggaagagat cgaactgatg
     4561 acagatcgtg tgtatgtgat gcatcagggc gaaattaccc actctgcact gaccgggcgc
     4621 gatattaatg tcgagactat tatgcgcgtt gccttcggcg atagtcagcg tcaggaggca
     4681 tcatgctgaa gtttattcag aacaaccgtg aaatcacggc attgctggcg gtggtgctgc
     4741 tgtttgtatt acccggtttt ctcgaccgtc agtatttaag tgtgcaaacg ctgaccatgg
     4801 tttatagcag cgcgcaaatc ctgatcctgc tggcaatggg cgcgacactg gtaatgctta
     4861 cgcgcaatat tgatgtttca gtgggttcga ttaccggaat gtgcgcggtg ctgttgggga
     4921 tgttactgaa cgcaggatat tcactacctg ttgcctgtgt cgcgacttta ctgcttggtt
     4981 tgctcgcggg atttttcaac ggtgtcctgg tcgcgtggct aaagatccct gccattgttg
     5041 ccacccttgg cacgttaggg ttgtacagag gcatcatgtt gctgtggact ggcggtaaat
     5101 ggattgaagg gttacccgcc gaactgaaac agctctccgc accgctgctg tttggcgttt
     5161 ccgcgattgg ttggttgacg ataattctgg tggcatttat ggcctggctg ctggcaaaga
     5221 cggcgtttgg acgcagtttt tatgccacgg gcgataattt acagggcgct cgtcaactgg
     5281 gtgttcgtac ggaagccatt cgcattgtgg cattttcgtt gaacggctgc atggcggcac
     5341 tggcgggaat tgtgtttgct tcgcagattg gttttatccc caaccagacc ggtaccgggc
     5401 tggagatgaa agcaattgca gcctgcgtgc tgggcggcat tagtttgctc ggcggttccg
     5461 gtgcgatcat cggtgcggta ctcggcgcat ggttcctgac gcagatcgat agcgtactgg
     5521 tgctgttgcg cattccggca tggtggaacg attttatcgc gggcctcgtg ctgctggcgg
     5581 tgctggtgtt tgatggacgc ctgcgttgtg cgctggaacg taatctacgg cggcaaaaat
     5641 atgcccgctt tatgacgcca ccgccatccg ttaaacccgc ttcgtcaggt aaaaaacggg
     5701 aggccgcata atgcgtattc gctacggttg ggaactggct cttgccgcac tgctcgttat
     5761 tgagattgtc gcatttggtg caattaaccc gcgaatgtta gatctcaata tgttgctgtt
     5821 cagcaccagt gactttatct gcattggcat tgtcgcccta ccgctgacga tggtgattgt
     5881 cagtggcggg atcgatattt cgtttggttc gaccatcggc ctctgcgcca ttgcattggg
     5941 cgtactgttt caaagtggtg tgccgatgcc gctggcgata ctcctgacct tactgctcgg
     6001 cgcattgtgc gggctgatca acgccggatt aattatctat accaaagtta acccgctggt
     6061 gattacgctt ggcacgctgt atctgtttgc cggaagcgct ctgctgcttt ccggtatggc
     6121 cggagcgacg gggtacgaag gtattggtgg attcccgatg gcgtttacag atttcgctaa
     6181 cctggatgtg ctgggactcc ccgttccgct gattatcttc ctgatatgtc tcctcgtttt
     6241 ctggctctgg ctgcataaaa cccatgccgg acgtaatgtg tttttgattg ggcaaagccc
     6301 gcgcgtggcg ctttatagcg cgattccagt taaccgcacc ttatgtgcgc tctatgccat
     6361 gacggggctg gcgtctgcgg tcgccgctgt gctgctggta tcgtattttg gttcagcacg
     6421 ttccgatctc ggtgcgtcgt ttctgatgcc cgccatcacc gccgtggtgc ttggcggggc
     6481 caatatttat ggtggttccg gttccattat cggcaccgcc attgcggttt tattagtggg
     6541 atatttgcag caaggtttgc aaatggcagg agtgccaaat caggtgtcca gcgccctttc
     6601 cggtgcgcta cttatcgtcg ttgtcgtagg tcgttccgtt agcctgcatc gccagcaaat
     6661 taaagagtgg ctggcgcgtc gggccaataa cccattgcca taaaggatat cttcatgaca
     6721 cttcatcgct ttaagaaaat cgccttactt agcgctcttg gcattgccgc aatctctatg
     6781 aatgtgcagg ccgcagagcg tattgcattt attcccaaac tggttggcgt gggatttttt
     6841 accagcggtg gcaacggcgc acaacaagcg ggtaaagagc tgggcgttga tgtgacctac
     6901 gacgggccga cagaacccag tgtttctggt caggtacagt tgattaataa cttcgtcaat
     6961 caaggttata acgccattat cgtttctgcg gtttcgcctg atggcttgtg tccggcactg
     7021 aaacgcgcca tgcaacgtgg tgtgagagtg ctgacctggg actctgatac taaaccggag
     7081 tgccgctctt actacattaa tcagggaacg cccgcccagt tgggaggtat gttggtggat
     7141 atggcggcgc gtcaggtgaa taaagacaaa gccaaagtcg cgtttttcta ctcaagcccc
     7201 accgttacgg accaaaacca gtgggtgaaa gaagcgaaag cgaaaatcgc caaagagcat
     7261 cctggctggg aaattgtcac tacgcagttt ggctataacg atgccactaa atcattacaa
     7321 accgcagaag gaatattaaa agcgtatagc gatctcgacg ccattatcgc ccccgacgcc
     7381 aacgccctgc ccgctgccgc acaagccgca gaaaacctga aaaatgacaa agttgcgatt
     7441 gtcggattca gtacgccaaa cgtgatgcgt ccatatgtgg aacgcggcac ggtgaaagaa
     7501 tttggcctgt gggatgtggt tcagcaaggc aaaatttcag tgtatgtcgc ggatgcatta
     7561 ttgaaaaaag gatcaatgaa aacgggcgac aagctggata tccagggcgt aggtcaggtg
     7621 gaagtctcgc caaacagcgt tcagggctat gactacgaag cggatggtaa tggcatcgta
     7681 ctgttaccgg agcgcgtgat attcaacaaa gagaatatcg gcaaatacga tttctgatgt
     7741 gcattactta accggagtaa gttatggcag atttagacga tattaaagat ggtaaagatt
     7801 ttcgtaccga tcaatcgcaa caaaatatcc cttttatcct gaaaggttgc ggtgcgctgg
     7861 attggggaat gcagtcacgc ttatcgcgga tatttaatcc gaaaacgggt aaaaccgtga
     7921 tgctggcctt tgaccatggt tattttcagg ggccgactac cggacttgaa cgcattgata
     7981 taaatatcgc cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc
     8041 gcagcgtagt tcccccagcg accaataagc cggtggtact gcgggcgtca ggagcgaact
     8101 ctattctggc ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc
     8161 tgaacagttg cgcggtggcg gcgcaggttt atatcggcag cgaatatgaa catcagtcga
     8221 tcaaaaatat tattcagctg gttgatgccg gaatgaaagt gggaatgccg accatggccg
     8281 tgactggcgt gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa
     8341 tcgccgctga aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac
     8401 ggattgttgc cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc
     8461 gcgaggcgct ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg
     8521 ggcgtaatat tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg
     8581 ttcaccataa cgaaacggca accggaacag gcactcgatc tgatttttgc taatgcctca
     8641 ctgcaatggc tgcccgacca ctacgaattg tttcctcatc tggtttcttt acttaatccg
     8701 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     8761 cgcgaagttg cctgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     8821 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     8881 tactatcacc agatgccgtc gcactaggcg attatcgatt gggtgaccgc cactggatta
     8941 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     9001 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     9061 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     9121 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     9181 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     9241 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     9301 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     9361 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     9421 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa actggatatc
     9481 tgaaagctgc ccattgcggc gctgaaccgc caaccactcc cccattatta acaagatacg
     9541 gttagctgga gagttcgaag ccagtacagg ctgaagatct tcagtcttga gataatgagc
     9601 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     9661 ttgcaaccgg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctcgcg
     9721 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     9781 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     9841 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaaaataac
     9901 agcgatagaa aagagaaaat tgagtagtaa gcgcgatgat atcttggata atactgagcc
     9961 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
    10021 caggcgtgaa aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
    10081 acaaattacc tacaagctta caacattaac aggcgctgca tgtggcatct gttaatgata
    10141 aaggcacata ggatgttgta actaatatag tgaaataaga aacccggtcg aaaccgggtt
    10201 cagaagtaac ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga
    10261 atcgcatcca ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc
    10321 tcccagtggt ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg
    10381 tcacgatgct ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc
    10441 ggatatgttt ctccgttacg ctcaccacga tagaaggcaa tcaatgccgc taatgcgaaa
    10501 gtaaggcgcg ccggaagtgt gccttttgcc ttctgccgtg ccagcagctg cggcaggatg
    10561 cgagtgcgga acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac
    10621 gggttacgga aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa
    10681 tccagtaccg gaataatttc ttcgtagata gctttttcaa cgaatgcgca aatttcagca
    10741 tcgttcatcg cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg
    10801 gtgtgcgcac cgttgaggat tgccactttg cgttctttgt acggtttaat atcgtcaaca
    10861 atcagcacgt tgagcggata tttgtccaga cgcagttcag tcgctaagga tttcggtccc
    10921 tgaatcacaa acaggtaaaa gtgttcagcg gtgtcgagaa aaccatcgtg ataacccagc
    10981 tcttcttcca gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc
    11041 gtagaacaga agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac
    11101 tcttgcgcat agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc gatcaactca
    11161 cacgggatga tgatccaacc tttatccagc gcaccgttga aatggctaaa acgttcgaac
    11221 agcagacggg tcagttttgc cggataactt accgctggcg cgtcatcgaa tttatcgccc
    11281 gcatggtagc tgatacccgc ttcggtggta ttagagaaaa caaagcgcat ttccgggttg
    11341 tgagccagtt tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag
    11401 cgaatcagac gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg
    11461 gtatacagac catcctgcgt gctcagtgac ggtgggaatg aagtttcaat cggacgaaca
    11521 acgaccacac cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca
    11581 aaggcgcgca ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg
    11641 ggaaaatcgc gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc
    11701 tgtttgaatt ggtacgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa
    11761 ctcctgatga ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg
    11821 aggaaatacg ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg
    11881 gaatgaataa agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg
    11941 cccatctttc ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc
    12001 atcggtcagc gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata
    12061 aagcgttttt tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc
    12121 cattagcaga ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc
    12181 catgcgcgcc accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct
    12241 taacacttta tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat
    12301 atcaagcagc atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc
    12361 ataaagaccg gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac
    12421 ttgcttcatt agcgaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc
    12481 catcgtggca ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac
    12541 cgagatatca atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt
    12601 caacagacat gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg
    12661 gaacgccaga ctccccgctc cgccaatgac taccgaggca ataaccgaca caataagcgc
    12721 gatggcgggc atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt
    12781 cagcatacaa ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag
    12841 caaaggccag aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca
    12901 taataatgcg gcaatcagac agtaattaaa tagccgtaag gcacttactg gctcatactt
    12961 atttttcccc agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat
    13021 attggagaaa ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac
    13081 cagcatcgcc acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc
    13141 aaacaccccc gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg
    13201 taactctgca ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg
    13261 tagcagatga ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta
    13321 tctgtccata caattgcggg tggcaattat ctcaacgtaa taagtaaatt ctcataaagg
    13381 atagcatgtt cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt
    13441 tttccgcaat tctcatggct gattcatctg tacaaccagc cagtgataga ttaccgccac
    13501 gatataacgt tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc
    13561 cagacaaccg cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg
    13621 catgggggtt tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa
    13681 atccgccgca tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt
    13741 catgcccaga cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc
    13801 gagagactgc attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggca
    13861 atacccgcaa gcgagttacc tgcatcatcc agttccggac tccagacagc gatggccatt
    13921 tcatgcggaa caatcgccac aataccgcca ccaacgccag atttcgccgg tagccccacc
    13981 cgccaggcaa actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt
    14041 tgccgcgcct gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta
    14101 gccagaaaga caaacgtcct ggccagctct acacagctca ttttcagagc gcagtaatga
    14161 aagtagtttt gcagaacggt tgtcacatca tgatggaaat tgccaaacga cttcatcagc
    14221 caggcgatag ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg
    14281 tatgaaatat cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt
    14341 gcgcttaatc gcccttgcaa catatcgcaa accaccagcg caccagcatt aataaacgga
    14401 ttacgcggaa taccctgctc catttccagt tgcactaaag aattgaacgg tgatccagac
    14461 ggatctttgc cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg
    14521 agactcagca ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga
    14581 aaaagctgtc cgtcaacggt acagatagca atgcccaatc gggaaccgtc tactgtagcc
    14641 agcgccggaa tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag
    14701 atgttttcta aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt
    14761 ctgaaaagac ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac
    14821 accgtctgga tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca
    14881 ctctttttca cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac
    14941 accccaccgc attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa
    15001 atggtcgctg aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct
    15061 ttcgcaacgg tgattgccgc aacagggcca aacatttctt cccgaaacgc ggtcatttct
    15121 ggggtaacat tcgccagaac cgttggcgga tagtagttac ctgccccagc catcttttcc
    15181 ccgcccagta acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca
    15241 tcacgtaaat caaaacgagc cattggtccg agagcattct cttcatcacg gggatcgccc
    15301 attttcaagg ctgccgcagc tgccacaaaa cgttcggtaa atgcagaagc aattccctct
    15361 tcgataataa agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct
    15421 accgccgctt taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa
    15481 ccgcccagtt ccagtacgca ttttttcagt gccgctccag cctgtgcgcc aatagccgct
    15541 cccgcacgaa cacttccggt caccgtgaca gcagcaatgc gcgaatcttt aatcatttga
    15601 ctgacaccgt cgttgtcggc attcagccag ccatatacgc cttgcgggat tcccgcatct
    15661 ttaaacacct gggcaatgag ctgagcacag cccatcacat tcggcgcatg tttaagtaag
    15721 tagccgttac ctgcaagaat gatgggaacc gcgccacgca tcacctgcca taacggaaag
    15781 ttccacggca taatcgccag aatcgtcccc aacggtcgat actcaataac tgcctgctga
    15841 ttttccacca gcgtaggttc cgccttcagc attgccggac catgttctgc ataccagtca
    15901 cacaaattcg ccgatttcgc cacttcagcg cgcgcctgat tgattggttt gcccatttca
    15961 cgggtgatca tttgcgccat ttcttcgcta cgggcgcgca gagccttacc gataccacgc
    16021 agtttttcag cacgataatc tatatttgtc tcgcgccagt cgcgaaagcc tgctgccgcc
    16081 agctgaagtg cgttttcgat atcgttagcg ccagcccacg gcagcacaga aagttgttca
    16141 cccgtggcag gatttattga aattgcatga gttgccggag taatggtcat cggggtatct
    16201 cctttatgag tcatggtatg aagatacgca gatttactct tgctttaaaa tgaataatat
    16261 taagccactt attcacgaat cgagaatgtt atggatctga cccaactgga gatgttcaac
    16321 gcggttgccg aagctggcag cattacccag gctgcagcaa aagtgcatcg cgtaccgtcg
    16381 aatctcacta cccgtctacg ccagctggaa acagaactgg gggtagatct gtttattcgc
    16441 gagaatcagc gtttacgtct ctctcctgcc gggcataact ttttacgcta tagccaacaa
    16501 attctcgcgt tagtggatga agcgcggagc gttgtcgctg gcgatgagcc gcaaggttta
    16561 ttttctcttg gttcgctgga aagcaccgca gcagtgcgca ttccggccac gctggcggaa
    16621 ttcaaccatc gttatcccaa aattcagttt tcactttcca ccggcccttc cggcactatg
    16681 ctggaaggag tgctggaggg aaaactgaat gcggcattta ttgatgggcc cattaaccat
    16741 actgccattg acgggatacc ggtataccgc gaggagctga tgatcgtcac gccacaaggt
    16801 catgcgccag taatccgcgc cagtcaggtt aacggcagta acatttatgc cttccgcgcc
    16861 aattgttcgt atcgtcgcca cttcgagagc tggtttcatg ctgacggtgc cgctccggga
    16921 actattcacg agatggagtc gtatcacggg atgttggcct gtgtggtcgc aggagcaggc
    16981 attgcgctta ttccacgctc tatgctggaa agtatgccgg ggcatcacca ggttgaagcg
    17041 tggccgttag ctgagcaatg gcgttggtta acaacctggc tggtctggcg tcgcggtgcg
    17101 aaaacgcgtc cgcttgaggc atttattcaa ctactggatg tgcctgactc ggcaaaacag
    17161 ggttaccaat gagctatttt tgatagttct tgcagctcaa atgccctata tagtgatgtt
    17221 ccgatgactt atgactatat ggggcaaata tggttacgcc agtaagcatc agtaattaca
    17281 tatctcttcc tgatgatttt cccgtacgta atattgcacc tcaggtaaaa gaagttttaa
    17341 aggactttat tgatgcgcta agtacaataa tatgtgatga agagtggcgc acgtctttaa
    17401 acatcaactc agccacaaaa aagatattta ataaccttga taacttatca tatattcaga
    17461 ggacttcttt tcggggtaac gacacgctat acaatgaaaa ggttcagttt aaactcactt
    17521 atcccgtcaa gaatggaaga cacaaagaaa atattgaatt tcaggtagta ataaatttaa
    17581 gccctattta tttagataat tttcgccatg atggagaaat taatattttt gcgctcccaa
    17641 cccaaagcct gtcactatgg ggcgcgtatt tcagaccggc gtcgagcgtg tgctatttct
    17701 gtttctgaat gattttattg aacaatttcc aatgatcaac cttggcgttc ccatcaaaag
    17761 agcgcataca ccacatattg aacccctgcc tccggatcgc cataccgctg cagattactt
    17821 acgccagttt gatttgcttg tcctgaattt tatctctcgc ggtaattttg tcatactccc
    17881 ccgattatgg agtaactctg aggttcacag atggttcgtc aataaagatc ctaacttgat
    17941 caccgccatt ctcgacataa cagacggcga attaaaagag gatttattgc aaagcctgat
    18001 ggattcatta ggttctaaca aacatgtact acccgaagtc tgcatctgct ttttatccct
    18061 tttagcagaa caagaatctc ctcattttca ggactttttt ttatttttcg ccaatatgtt
    18121 attgcactat caccaattta tgaatcccaa tgaaagtgat ctgaatgacg tgttgatgcc
    18181 agcatcatta agtgatgata aaattatcaa acatatggcg cgcaggaccc tcaaactgtt
    18241 tgtaaaaaat gaaacgccgc caaaagttac tcacgaagac ctggtgaaaa acaggcctcg
    18301 ctcccctgtc agaccaccta tacccgcaac cgccaaaacg ccagacctcc ctgaacgtca
    18361 ttaaaccgtg atgttaccga ctctctgacg cggaacagaa tcagcgtcag agaaacggaa
    18421 aacgcgatcc agatcacaaa tgcattgtat tcacatcatt aaccgtttta agatcatttc
    18481 atcacttttt cgcaactcac ccgataatct gttatgacaa caaacactgt ttcccgcaaa
    18541 gtggcgtggc tacgggtcgt tacgctggca gtcgccgcct tcatcttcaa caccaccgaa
    18601 tttgtccctg ttggcctgct ctctgacatt gcgcaaagtt ttcacatgca aaccgctcag
    18661 gtcggcatta tgttgaccat ttatgcatgg gtagtggcgc taatgtcatt gccttttatg
    18721 ttaatgacca gccaggttga acggcgcaaa ttactgatct gcctgtttgt ggtgtttatt
    18781 gccagccacg tactgtcgtt tttatcgtgg agctttaccg ttctggtgat cagtcgcatt
    18841 ggtgtggctt ttgcacatgc gattttctgg tcgattacgg cgtctctggc gatccgtatg
    18901 gctccggccg ggaagcgagc acaggcattg agtttaattg ccaccggtac agcactggcg
    18961 atggtcttag gtttacctct cgggcgcatt gtgggccagt atttcggttg gcgaatgacc
    19021 ttcttcgcga ttggtattgg ggcgcttatc acccttttgt gcctgattaa gttacttccc
    19081 ttactgccca gtgagcattc cggttcactg aaaagcctcc cgctattgtt ccgccgcccg
    19141 gcattgatga gcatttattt gttaactgtg gtggttgtca ccgcccatta cacggcatac
    19201 agctatatcg agccttttgt acaaaacatt gcgggattca gcgccaactt tgccacggca
    19261 ttactgttat tactcggtgg tgcgggcatt attggcagcg tgattttcgg taaactgggt
    19321 aatcagtatg cgtctgcgtt ggtgagtacg gcgattgcgc tgttgctggt gtgcctggca
    19381 ttgctgttac ctgcggcgaa cagtgaaata cacctcgggg tgctgagtat tttctggggg
    19441 atcgcgatga tgatcatcgg gcttggtatg caggttaaag tgctggcgct ggcaccagat
    19501 gctaccgacg tcgcgatggc gctattctcc gggatattta atattggaat cggggcgggt
    19561 gcgttggtag gtaatcaggt gagtttgcac tggtcaatgt cgatgattgg ttatgtgggc
    19621 gcggtgcctg cttttgccgc gttaatttgg tcaatcatta tatttcgccg ctggccagtg
    19681 acactcgaag aacagacgca atagttgaaa ggcccattcg ggcctttttt aatggtacgt
    19741 tttaatgatt tccaggatgc cgttaataat aaactgcaca cccatacata ccagcaggaa
    19801 tcccatcaga cgggagatcg cttcaatgcc acccttgccc accagccgca taattgcgcc
    19861 ggagctgcgt aggcttcccc acaaaataac cgccaccagg aaaaagatca gcggcggcgc
    19921 aaccatcagt acccaatcag cgaaggttga actctgacgc actgtggacg ccgagctaat
    19981 aatcatcgct atggttcccg gaccagcggt acttggcatt gctagcggca caaaggcaat
    20041 attggcactg ggttcatctt ccagctcttc cgacttgctt ttcgcctccg gtgaatcaat
    20101 cgctttctgt tgcggaaaga gcatccgaaa accgataaac gcgacgatta agccgcctgc
    20161 aattcgcaga ccgggaatcg aaatgccaaa tgtatccatc accagttgcc cggcgtaata
    20221 cgccaccatc atgatggcaa atacgtacac cgaggccatc aacgactgac gattacgttc
    20281 ggcactgttc atgttgcctg ccaggccaag aaataacgcg acagttgtta atgggttagc
    20341 taacggcagt aacaccacca gccccaggcc aattgcttta aacaaatcta acattggtgg
    20401 ttgttatcct gtgtatctgg gttatcagcg aaaagtataa ggggtaaaca aggataaagt
    20461 gtcactcttt agctaacctt gcatcgcatt gaccaaaact tgaaccgatt tagcaaaacg
    20521 tggcatcggt caattcattt atttgactta tacttgcctg ggcaatatta tcccctgcaa
    20581 ctaattactt gccagggcaa ctaatgtgaa aagtaccagc gatctgttca atgaaattat
    20641 tccattgggt cgcttaatcc atatggttaa tcagaagaaa gatcgtctgc ttaacgagta
    20701 tctgtctccg ctggatatta ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc
    20761 ggcgtgtatt actccggttg aactgaaaaa agtgttgtct gtcgaccttg gagcactgac
    20821 ccgtatgctg gatcgcctgg tctgtaaagg ctgggttgaa aggttgccga acccgaatga
    20881 taagcgcggc gtactggtaa aacttaccac cagcggcgcg gcaatatgcg aacaatgcca
    20941 tcaattagtt ggccaggacc tgcatcaaga attaacaaaa aacctgacgg cggacgaagt
    21001 ggcaacactt gagcatttgc ttaagaaagt cctgccgtaa acaaaaaaga ggtatgacga
    21061 tgtccagacg caatactgac gctattacca ttcatagcat tttggactgg atcgaggaca
    21121 acctggaatc gccattgtca ctggagaaag tgtcagagcg ttcaggttac tctaaatggc
    21181 acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt aggccaatac atccgcagcc
    21241 gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa cgagccgata ctctatctgg
    21301 cagaacgata tggcttcgag tcgcaacaaa ctctgacccg aaccttcaaa aattactttg
    21361 atgttccgcc gcataaatac cggatgacca atatgcaggg cgaatcgcgc tttttacatc
    21421 cattaaatca ttacaacagc tagttgataa cgtgacaacg tcactgaggc aatcatgaaa
    21481 ccactttcat ccgcaatagc agctgcgctt attctctttt ccgcgcaggg cgttgcggaa
    21541 caaaccacgc agccagttgt tacttcttgt gccaatgtcg tggttgttcc cccatcgcag
    21601 gaacaaccac cgtttgattt aaatcacatg ggtactggca gtgataagtc ggatgcgctc
    21661 ggcgtgccct attataatca acacgctatg tagttattgt caggctctga tcacagagcc
    21721 tgacatctcc gtaatatctt cctcaagtct caaatatttt cgccattgtt ggcgatgaga
    21781 tcccgatacc agtaaaacga cgctttacgt atccgttcca gcgttccctt cccttcgttg
    21841 tctttatcga cataaatcat tccgtagcgc tttttcattt cccctgttcc ggcagaaacc
    21901 aggtcaatgc agccccacgg ggtgtagcca attaagtcaa caccatcttc aactacggct
    21961 tttttcattt cccgaatatg ggaagcaaag taatcaatgc gatagtgatc gttcaccgtg
    22021 ccgtcagctt gtctctggtc aaccgcacca aatccatttt cgacaataaa cagcggcaac
    22081 tggaaatgat cccagaacca gttgagtgaa taacgtagcc ctgccggatc aatttgccat
    22141 ccccattcgg atgtatcgat ataagggttg ctgaccaaat ctcgtggttc aacataatcc
    22201 agttgcgggt tatcgggcga aaattgtgtc gtaaaagaca tgtagtagct aaagccgata
    22261 aagtcgacac aaccactggc aagaatcgcg ttatcttctg gtgtgatatc gaggttgaat
    22321 cctttcctgg caaagtaatt cagcatatgt tgcggataat atccacgagc atgaacatca
    22381 gtaaaccagt aacgacgatg cattgctttc gtggccatca tcatatcgtt gggtgcacac
    22441 gtcagaggat agatgggaca catggcgatc atacagccaa tgttaaattc aggattgatc
    22501 gattttccag tctgtaccgc cagggcactg gcaactaact cgtaatgtac cgcctggtac
    22561 attatttgtt cgcgctcatt gatatcttcc tctggcgaat acaagatacc ggaattagta
    22621 aatggacaca ggctttcgct gaaattcacc tgattattga tttcgttaaa cgtcatccag
    22681 tactttactt tttctttata gcgcgtgaag accgttgatg cgaagcggat aaaaaagtcg
    22741 atcagtttac ggtttcgcca gccaccatat tttgtcacca gatgataagg catctcaaaa
    22801 tgtgaaagcg tcaccacagg ttccattccc tgcttaaggc attcatcgaa cagatcatca
    22861 taaaattgta aaccctcttc attcggctcc tgttcgtcac cttgcggaaa gattcgtgtc
    22921 caggcaatgg aagttcgaaa gcatttgaat cccatctcgg caaataactg aatatctgtt
    22981 ttatagcgat gataaaaatc aattgcttca tgattgggat aattaagccc gtcgataacg
    23041 ccttctgtca cttcacgcgg caccccgtga gcgccagcag tcatcacatc agcgatgctg
    23101 atgccttttc ctccttcatt ccagccacct tccaactgat gcgcggctac cgcaccgccc
    23161 cataaaaaac cttttttaaa tcctgacata cacaactcct taactaaaat agattaccac
    23221 cagacttccg cctggatgcc ggccataaac tcgtctttgc tgttctcatt aaacttaaaa
    23281 ttcgataatt cgttatccag aatattgata tacgttccat agaagcgaat ttctggtcgt
    23341 gaacctaaaa tgctttcacc cactttcaat gcatgccaga gtgtcgtttt ataagccgat
    23401 tcatttagtg ttacaccctg ctgagttttg ttctgttgct taaaccagcc taattcaagc
    23461 cccgtctgat tccatgtatt ccagatccag gccggtctta ttacagtgcg aatactgtca
    23521 aaatcactat gagcaccact ttcataacta taaacatctt cgccgtgaga atagacaagc
    23581 gcgttagcca taataatatt gtcagtaaga tacatctcgc cctgagagat taaacgccag
    23641 gcgatcccat tggtatgatc accatagtaa tagcgaccat gcgccatcgt attactggca
    23701 tctgagaaac tggcaaaact gctggcatag gaattattgg caacctgtaa cgtaaattca
    23761 ttaaacgtat cgcgttgcag gttttgccgt aaaacagaag tcagcatcca gctatctttc
    23821 atttcaaaat aactgttgtc attttcgtta tcttgttgtt gatccgtttt attaggtgcg
    23881 gaatatttag ccattaatga taatgtcgca tcatcccata acgggatatt gcgataacga
    23941 acatccacag aattggtatt catctggctg gtacgcgaaa aatcacgaga gtaaacatcg
    24001 acatcatcac ggcttaagga catatcaaac agccctacac caagtgccca gttttcaatc
    24061 cccacacccg cggcgacatc cgtggttaag gttttccagt ccagcatctg gatctcatat
    24121 tgcgggagtt tatgtttgcc gacccagaag tctgcctctg gtgcgaaggg taaaaaacct
    24181 cgcgttgtca ggtaaatatc gctgaactgc atgatatttt cattggcact gtcaccaaac
    24241 caggcatcgt tatactgctc acccacgttg ccatcatagg taacgaccgc atttgccgtt
    24301 ttaccgtcct gattataaac acgctgattt aaggtcaggt caaaccaacc gctcatctca
    24361 ttaccaaaac gtcccagaga acccgctgca taagtttgtg gtgagccgtg attaccagta
    24421 ccccagcctg agcggaagta cccctgatag ctgaacccaa tatcatcctt tatatattta
    24481 ctgatatctt tcagggtcac gctttcgata gtggttttgt cgcctttact ttcaataacg
    24541 gcaacttgct gcgatccatt tatagtatgc gctgcggtaa acgagttctg ttcaccgttt
    24601 tcatttttga tgttatcagc gacgggagat gtcgctgata tttcagcaag cgaggctgat
    24661 ttatatttgt tttccgtgat gcttttttgt aaggtcgaaa gtcgggattt atatactccc
    24721 agttcattct gtgttgcttt cagctcttgt ttattttgcg acaattcatt ttcaagcaat
    24781 tcaaggcgct gctctatggt taattttgtt gccatcgcgt tcaatggaaa caatgctgta
    24841 ataatagaaa aagacaaaag gctgacgtta agcgtcttta tattcatgac ttatccttaa
    24901 gagtataata atttcctcca gctatcgcac tggaggaaaa aatcaggcat ggagttcttt
    24961 tcttaatgaa ataatttccc tggctaaatc ctggcataat attgcattca tcaaatgatc
    25021 ctgtgcatga atcagaatta aattaacttt tattttgcca cagccttcat cctgactaat
    25081 taatgcggtc tgaatcttat gtgcctgaag acaggcagat tcagaactgg ccattagctg
    25141 agttgcgccc tgccagtctt tttgcccggc acaacgtatg gcttccatcg cgtcagaacg
    25201 tgcctgcccc gcgttgacca gtaactccat aacaagttct tcatctgcaa acatcacaat
    25261 ctccttcctg ggtaaatgga attatccgtt agttgtcatg ttattgactt tgttggcggc
    25321 aataacaaaa ggtaaataga tcattgtcgc cacacctaaa cataaaaaac caaccagtaa
    25381 agccagccag ttccccccgg ttccgaggaa agcaattaaa ggtccgggcg tagtccaggg
    25441 cacggcataa gcaaccggtg gaataatttc catcgaaaca aagaagtaac caatggcaca
    25501 gttaacaatg ggaaccataa taaacggcac catcatgatg gggttaagta caataggaag
    25561 gtcaaacatt atcggttcat tgatattgaa aataccgggg ataaatgaca tttttgccag
    25621 atcacggtat tccgcacgct tagaagcgat aaaaatagcc aacaataacc ccaacgtcat
    25681 acctgaaccg ccgcagttgg cgaatgcatc attaatagaa gtccaggtaa tcggatatgg
    25741 cgcgccccag gtagtgccat gttgagcggc ccaggagaga ttctccagat tagcctcaga
    25801 aaaaacggtt tctcgaattg ccgatgtggt attaggtccg tggatcccca gcacccacag
    25861 gaaattaccc acagccccca ggataattac cgcaaagata ttcgttccca tatgtttgag
    25921 cggcgtctgg attaatgtgt aaataagatc gtttagtcct gccggggata tcagtgtcag
    25981 gcaataatta agtgcggaaa agaacaccat gacaaaaaat attggcaata aaactttaaa
    26041 tgaacgcgct accgcaggcg gtacagctgc cggcatcgtt atggtgattt ttgggtttcg
    26101 agctaagcga caaaatattt cactggtgat caatgcaacg ataacagcaa caaacaaccc
    26161 ctgcgcgccg agatatttgg tcgtcaggaa tgcttgccca tctatcatct gatatggcgt
    26221 ataaacaata aaaaatgctc ctattgccgt taatccgcat aaaagatcat cttgctcata
    26281 gctaatcgcc atattcctgg cgaccaggaa agcaatcata attgacatga tatttacaga
    26341 accattcatt accggagtaa aaatagcttg tgctttttca aggttgggga aaatgctatt
    26401 cagatgcagt aatccggcaa taaaaccgtc tggcgataat atggcaaagt taattaatat
    26461 aattaatgaa cttgccatga taattggaaa cgtaaaaacg aatccatctc tcaccgctgc
    26521 aacatgtttt tgtgagttta acttgatagc aactggaaca agaaaacgtt ccattccacg
    26581 ttcgaatgag gccattaatc ccattttttt atacctgttg ttttggatat ttatacatcc
    26641 caataaagca tgtatgccat atccatttta tgatgtgtga aaagttagtt caggtaatag
    26701 tttaataata ttagattctt actcttgcag tagtttcatc gcaaattcaa gaactgcctg
    26761 tccgttcatg gtgccgtagt ctttcatctc gataacggct acaggcattt tcccctgtgc
    26821 aacggcttca atctctggtt tctgaaagcg aacttgcggg ccaagtaaaa caacgtcaac
    26881 ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc aatgcggaaa tattaacttc
    26941 aagtgaaata gcgttagcat gatcaatcat acgtttaacc agcatactgg ttgacatgcc
    27001 cgcagcgcaa actaacaata tttttttcat attttccctt actggtatat aacagattac
    27061 attgtttaaa tatcatatcc cgcagtagct atacgtcctg gtacattgat cacagtaata
    27121 cagtgataaa gttgttcaac atcagggtga ttattaaacg actcggccat acgataaacc
    27181 aatcaggact caactccttg ttttcaatgg ctcttaaaag gtgcaaaagt gatttttcaa
    27241 aagattgccc gcttacttaa atccgaaatc aatggcaatt catggcatgt tggtgatttg
    27301 ttgccgtcag aagcggaact cgctgttcgt tataatgttt cgcgtaatac cctgcgtaag
    27361 gcattatcct tgctggaagg cgaaggaatt attcacagaa agcatggttc aggaagatac
    27421 attcaaaaaa agaattttgt tgcacacatt gatcacatga acagtttcag tgaaattgcg
    27481 cataaaagcg gcaaagaggc agggagtcag attatgaaat ttgaagtgca ggatgcttca
    27541 cccactattg cgactgagct gaatttagtg actggtgagc aggtttatta cataaagaga
    27601 ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa cgtggatgtc agtagcacgt
    27661 tttcctgatt taaccgtgtc acatatgcaa aaatccaaat tttcatatat tgagaatgaa
    27721 tgcgggatca aaatcattgg cacctttgaa actttctccc ctacttttcc taccccagaa
    27781 atcgccagta ttttacggat cagcccacgg gatcccatac ttaaaattca gacccaggct
    27841 gtggacagta actctattcc gctggattat tcgttacttt acagcaatat tttcgagttc
    27901 caggtaaagt actttttccc gcgataaaag cagacgcaca gagtgctgac atcacattct
    27961 gtgcgtatgg tttagcctct aacctttacc gctttacgcc accgcaagcc aaatacattg
    28021 atatacagcc cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca
    28081 tccaacaata gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca
    28141 acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc
    28201 gccacaaacg ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg
    28261 tgaatcatgg atgcggaacc atcgagaatc agcgaggcaa caaagaaggg aatgattggg
    28321 attaaagcgc tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc
    28381 tttttattga agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag
    28441 ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa
    28501 atcgctaagg cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg
    28561 ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc
    28621 ataccaaagt taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca
    28681 taccccaaga gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa
    28741 gcgaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact
    28801 ttgatgacca caaaatttag cccccatacg accactacca gtagcgccaa caccccatct
    28861 tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat
    28921 ttacttttct ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat
    28981 aagtcgctat acttcggatt tttgccatgc tatttcttta catctctaaa acaaaacata
    29041 acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta ccagcgccct
    29101 tcttgcctcg tcgttgttat taaccatcgg acgcggcgct acgctgccat ttatgaccat
    29161 ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc
    29221 gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa
    29281 gaaacgctat atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt
    29341 agtgaataac gtgacgctgg tggtgctctt ttttgccctt attaactgcg cctattccgt
    29401 ttttgctacc gtgttgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa
    29461 aatcttctca atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg
    29521 cacgctgttg gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc
    29581 gtttcccatg cttttcattc aaatttgggt aaagcgcagc gagaaaatca tcgccacgga
    29641 aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac
    29701 ctgctctggt tttctggctt cctttgttag cggcgcattt gcttcatgca tttcacaata
    29761 tgtgatggtg attgctgatg gagattttgc cgaaaaggtg gtcgcggttg ttcttccggt
    29821 taatgctgcc atggtagtta cgttgcaata ttccgtgggc cgccgcctta acccggttaa
    29881 catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcggtctgg ttggttttat
    29941 tttttccggc aacagcctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga
    30001 aatcatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa
    30061 agccagctat ttttctgccc agtctttagg ctggcttggt gctgcaatta acccattagt
    30121 gagtggcgta gtgctaacca gcctgccgcc ttcctcgctg tttatcatct tagcgttggt
    30181 gatcattgct gcgtgggtgc tgatgttaaa agggattcgt gcaaaaccgt gggggcagcc
    30241 cgcgctttgt tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta
    30301 cctctgctaa tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca
    30361 ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat
    30421 aattttattt tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac
    30481 ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt
    30541 tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg gatagatttg
    30601 tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt tgacacagta
    30661 gcatcagttt tctcaatgaa tgttaaacgg agcttaaact cggttaatca cattttgttc
    30721 gtcaataaac atgcagcgat ttcttccggt ttgcttaccc tcatacattg cccggtctgc
    30781 tcttccaatg accacatcca gaggctcttc aggaaatgcg cgactcacac ctgctgtcac
    30841 ggtaatgttg atatgccctt cagaatgtgt gatggcatgg ttatcgacta actggcaaat
    30901 tctgacacct gcacgacatg cttcttcatc attagccgct ttgacaataa tgataaattc
    30961 ttcgcccccg tagcgataaa
//
LOCUS       DAZFKM010000048        30930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFKM010000048 DAZFKM010000000
VERSION     DAZFKM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHKAY_002271"
     CDS             <1..249
                     /locus_tag="ACHKAY_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5700942.1"
                     /translation="LHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNP
                     RHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK"
     gene            339..716
                     /locus_tag="ACHKAY_002272"
     CDS             339..716
                     /locus_tag="ACHKAY_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5700943.1"
                     /translation="MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            713..862
                     /locus_tag="ACHKAY_002273"
                     /pseudo
     CDS             713..862
                     /locus_tag="ACHKAY_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            962..1042
                     /locus_tag="ACHKAY_002274"
                     /pseudo
     CDS             962..1042
                     /locus_tag="ACHKAY_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1858..2229
                     /locus_tag="ACHKAY_002275"
     CDS             1858..2229
                     /locus_tag="ACHKAY_002275"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5700944.1"
                     /translation="MLFLPPLQLSAPGSIGFKNRRQLAAWLGLVPRQCFSVGKNTLGS
                     ISKRDDVYIRTLLFHGAKTVSNARKGRQVQESWLGRPVSRRNKNVATVALNNKNARVL
                     WVLLTNDKEISPEEVMVAAYM"
     gene            2500..2865
                     /locus_tag="ACHKAY_002276"
     CDS             2500..2865
                     /locus_tag="ACHKAY_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO5700945.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(2966..3295)
                     /gene="tmaR"
                     /locus_tag="ACHKAY_002277"
     CDS             complement(2966..3295)
                     /gene="tmaR"
                     /locus_tag="ACHKAY_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO5700946.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(3467..4525)
                     /gene="yeeA"
                     /locus_tag="ACHKAY_002278"
     CDS             complement(3467..4525)
                     /gene="yeeA"
                     /locus_tag="ACHKAY_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO5700947.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRHLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(4723..5196)
                     /gene="sbmC"
                     /locus_tag="ACHKAY_002279"
     CDS             complement(4723..5196)
                     /gene="sbmC"
                     /locus_tag="ACHKAY_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO5700948.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(5314..6480)
                     /gene="dacD"
                     /locus_tag="ACHKAY_002280"
     CDS             complement(5314..6480)
                     /gene="dacD"
                     /locus_tag="ACHKAY_002280"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO5700949.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            6689..8116
                     /gene="sbcB"
                     /locus_tag="ACHKAY_002281"
     CDS             6689..8116
                     /gene="sbcB"
                     /locus_tag="ACHKAY_002281"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO5700950.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(8159..8386)
                     /gene="tsuB"
                     /locus_tag="ACHKAY_002282"
     CDS             complement(8159..8386)
                     /gene="tsuB"
                     /locus_tag="ACHKAY_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO5700951.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(8400..9458)
                     /gene="tsuA"
                     /locus_tag="ACHKAY_002283"
     CDS             complement(8400..9458)
                     /gene="tsuA"
                     /locus_tag="ACHKAY_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO5700952.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(9637..10995)
                     /gene="plaP"
                     /locus_tag="ACHKAY_002284"
     CDS             complement(9637..10995)
                     /gene="plaP"
                     /locus_tag="ACHKAY_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO5700953.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(10985..11047)
                     /gene="yoeI"
                     /locus_tag="ACHKAY_002285"
     CDS             complement(10985..11047)
                     /gene="yoeI"
                     /locus_tag="ACHKAY_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO5700954.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(11262..12191)
                     /gene="yeeY"
                     /locus_tag="ACHKAY_002286"
     CDS             complement(11262..12191)
                     /gene="yeeY"
                     /locus_tag="ACHKAY_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO5700955.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(12237..13061)
                     /gene="yeeZ"
                     /locus_tag="ACHKAY_002287"
     CDS             complement(12237..13061)
                     /gene="yeeZ"
                     /locus_tag="ACHKAY_002287"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5700956.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(13144..13398)
                     /gene="yoeB"
                     /locus_tag="ACHKAY_002288"
     CDS             complement(13144..13398)
                     /gene="yoeB"
                     /locus_tag="ACHKAY_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO5700957.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(13395..13646)
                     /gene="yefM"
                     /locus_tag="ACHKAY_002289"
     CDS             complement(13395..13646)
                     /gene="yefM"
                     /locus_tag="ACHKAY_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO5700958.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            13929..13976
                     /gene="hisL"
                     /locus_tag="ACHKAY_002290"
     CDS             13929..13976
                     /gene="hisL"
                     /locus_tag="ACHKAY_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO5700959.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    13952..14075
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            14122..15021
                     /gene="hisG"
                     /locus_tag="ACHKAY_002291"
     CDS             14122..15021
                     /gene="hisG"
                     /locus_tag="ACHKAY_002291"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO5700960.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            15027..16331
                     /gene="hisD"
                     /locus_tag="ACHKAY_002292"
     CDS             15027..16331
                     /gene="hisD"
                     /locus_tag="ACHKAY_002292"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO5700961.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            16328..17398
                     /gene="hisC"
                     /locus_tag="ACHKAY_002293"
     CDS             16328..17398
                     /gene="hisC"
                     /locus_tag="ACHKAY_002293"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO5700962.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            17398..18465
                     /gene="hisB"
                     /locus_tag="ACHKAY_002294"
     CDS             17398..18465
                     /gene="hisB"
                     /locus_tag="ACHKAY_002294"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO5700963.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            18465..19055
                     /gene="hisH"
                     /locus_tag="ACHKAY_002295"
     CDS             18465..19055
                     /gene="hisH"
                     /locus_tag="ACHKAY_002295"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO5700964.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            19055..19792
                     /gene="hisA"
                     /locus_tag="ACHKAY_002296"
     CDS             19055..19792
                     /gene="hisA"
                     /locus_tag="ACHKAY_002296"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO5700965.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            19774..20550
                     /gene="hisF"
                     /locus_tag="ACHKAY_002297"
     CDS             19774..20550
                     /gene="hisF"
                     /locus_tag="ACHKAY_002297"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO5700966.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            20544..21155
                     /gene="hisIE"
                     /locus_tag="ACHKAY_002298"
     CDS             20544..21155
                     /gene="hisIE"
                     /locus_tag="ACHKAY_002298"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO5700967.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(21252..22229)
                     /gene="wzzB"
                     /locus_tag="ACHKAY_002299"
     CDS             complement(21252..22229)
                     /gene="wzzB"
                     /locus_tag="ACHKAY_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO5700968.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(22376..23542)
                     /gene="ugd"
                     /locus_tag="ACHKAY_002300"
     CDS             complement(22376..23542)
                     /gene="ugd"
                     /locus_tag="ACHKAY_002300"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO5700969.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(23791..25197)
                     /gene="gndA"
                     /locus_tag="ACHKAY_002301"
     CDS             complement(23791..25197)
                     /gene="gndA"
                     /locus_tag="ACHKAY_002301"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO5700970.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(25308..25715)
                     /locus_tag="ACHKAY_002302"
     CDS             complement(25308..25715)
                     /locus_tag="ACHKAY_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO5700971.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(25706..26914)
                     /locus_tag="ACHKAY_002303"
     CDS             complement(25706..26914)
                     /locus_tag="ACHKAY_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5700972.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(26914..28044)
                     /gene="wecB"
                     /locus_tag="ACHKAY_002304"
     CDS             complement(26914..28044)
                     /gene="wecB"
                     /locus_tag="ACHKAY_002304"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO5700973.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(28041..29147)
                     /locus_tag="ACHKAY_002305"
     CDS             complement(28041..29147)
                     /locus_tag="ACHKAY_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFO5700974.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(29149..30183)
                     /gene="fnlA"
                     /locus_tag="ACHKAY_002306"
     CDS             complement(29149..30183)
                     /gene="fnlA"
                     /locus_tag="ACHKAY_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     4,6-dehydratase/5-epimerase"
                     /protein_id="HFO5700975.1"
                     /translation="MFKNKTLVITGGTGSFGNAVLKRFLDTDITEIRIFSRDEKKQDD
                     MRKKYNNSKLKFYIGDVRDYNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVL
                     GTENVLEAAIANGIKRVVCLSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVIC
                     GTRYGNVMASRGSVIPLFVDLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNN
                     GDIFVQKAPAATIQTLAIALKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDM
                     GDYYRVPPDLRDLNYGKYVEHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRAL
                     RSGEDYELDS"
     gene            complement(30195..>30930)
                     /locus_tag="ACHKAY_002307"
     CDS             complement(30195..>30930)
                     /locus_tag="ACHKAY_002307"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5700976.1"
                     /translation="SVINQEKDSFVIVVDNCSNEQELKKLNDIESKYNEKVRLIKSDV
                     NLGYFGGLNLGLKGLPRNHPIVVGNNDLVYESNFTQIISQTTYPDDALVIAPNVITKD
                     GYHQNPHCRKRVSKFRKFLYDIYFSNYIAALVLTWGSRFLNYLKGGRNNSFDQGRGYI
                     HMGIGACYVLMPSFFKYFDELDNKVFLYGEEAYLAGQLMEVNGKIFYEPDAIVHHEES
                     ATLAKVASKTKYGYMKSSYYDYKKYL"
BASE COUNT         8356 a         7676 c         7127 g         7771 t
ORIGIN      
        1 ttgcattacc ttgccgaccg tgccggtatc agaggccggt tcagagatgc ggatgcgtac
       61 cctctggacc aggcctttcc gctgctgatg aaacaactga aattaatgct caccagtggt
      121 gaactgaatc cccgccatca gcataccgtg acactgtatg cgaaggggct gacctgtgaa
      181 gccgacaccc tcggctcctg tggttacgtt tatctggctg tttatccgac acctgaaacg
      241 aaaaagtaaa ccaccggaat aaccctctac tccggcctgg tgttttcacc acgccactgt
      301 cccatttttt atctctgcat atcaggaaaa tcttcagtat gaaaacatta cccgacacgc
      361 atgtacggga ggtatcgtgc tgcccgtctc tcgtcaccat ctggcagaca ctgctcaccc
      421 gactgctgga ccagcactac ggccttacgc tgaatgacac accgttcgct gatgaacgtg
      481 tgattgaaca gcatattgag gcaggcattt cactgtgtga cgcggtgaac tttctcgttg
      541 aaaaatacgc gctggtacgt accgaccagt cgggattcag cgcctgtacc cactctcagt
      601 taataaacag catcgatatc ctccgggctc gccgggcaac aggcctgatg acccgcgaca
      661 attacagaac ggtaaataac attaccctgg ggaagcatcc ggaggcgaaa cgatgaaact
      721 ggccctgacg ctggaagccg acagcgttaa cgtgcaggcg ctgaacatgg ggcgcattgt
      781 cgttgacgtc gatggtgtta atctcgctga actgattaac aaggtcgctg aaaacggtga
      841 ttcactccgc gtggtggatg aacgtgactc taccgaaaca ccggcaactt atgccagccc
      901 tcaccagttg ctgtaagaca atgcagtgat acagggtata gtgccattaa ccacagaaac
      961 agcactgttg tcctgtatta agcaggcaag agaagatgtc cttaccctgc gccatctgca
     1021 gcttctgcac cagaaccggt gaatctcact caacaggctt catttcccct gacgccagcc
     1081 tgaatacagc tggcgttttc atttataaac agaaaggaaa accgataata atggaatgga
     1141 cgcccctacc atgaagcaac ggtaggatat tactgataaa ccttccagtg gaggtgcgtt
     1201 atggccgttt taacgattag cattgacctg acaaaaaatg aattccaaat ccacggtctg
     1261 ggtaggaaca gaaaaatcta agctcagaaa acggattaaa cgttccctga tgcccgtatt
     1321 cttcgcgaat atgtcttcct gtcggattgg gatggaggtc tgcgccggag cgcgtcatta
     1381 gtgagaatgc tgtctactta ggggcataat gtcagactaa tgcccgcgat ttgtaaagcc
     1441 atatgtgaaa acaaacaaga atgatatggc tgctgccgag gccatctgtg aagccgttac
     1501 ccgacctaat atgcggtttg tcagcatcaa gacgcctgag cagtagtcat tactgtcttt
     1561 tcatcgtgcc caaacgggat ttgttaaatc aagggctgcg cagataaacc agatcagaga
     1621 attactggct gaatttggca tcgttttccc gcccgacatt gtagcattca gtcgtcatgt
     1681 ccctgccctg cctgaagacg cggaaaactg tttaacaatg ccgttcagac agctgtcgtc
     1741 ctatctctaa tgaaaatgtg aaacaactga gcgtacacat tgaaaccata gaagccgtga
     1801 gcaacgaaca cgtcgggaac gacgaactgt gtaggaaaat tgccaccatt tcggggattg
     1861 ctattcttac caccactgca attgtcagca ccgggtagta tcggcttcaa aaaccggcgt
     1921 cagcttgctg catggcttgg actggttccc cgacagtgct tcagcgtagg taaaaacacg
     1981 ctggggtcta tcagtaaacg ggacgatgtc tatatcagaa cgctactgtt ccatggtgcg
     2041 aagacagtgt caaacgccag aaagggcaga caggtccagg aaagttggct gggcagacca
     2101 gtctcgcggc ggaataaaaa tgttgccacc gtagcgctga acaacaaaaa tgccagagtg
     2161 ctctgggtat tgctaaccaa cgataaagag atttcaccag aagaagtgat ggtagctgca
     2221 tatatgtaaa tgaaactact tctgtctgtt gaaaaataaa accaacctac tgattgctaa
     2281 ggataaacaa gtattaaagg ccagactgat aagaccgcga ctaagtgaac ctgtatctga
     2341 catggcatag cttcgctaat gtgtcatcat gattaggact aaatctgtga attcctttag
     2401 gaagcacgga cacaggccaa tctgagtcca tatgtatgat tggaatctta cacagatgac
     2461 tttttgacac ggggacttgg caaacagggg cgaccatgta tgtcagatat gacaatctca
     2521 cttccgaaag aagtcaacgg gcacgttatc tgttcaacca gaatccgaca cattctggct
     2581 atacgaaaga gcatacttat gcaaatctat atgcttttcc ggcaacaagc aggaatctca
     2641 gcgctgaggc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca cccggtcatt
     2701 ggagtagtaa ccaaagcaga tttagccagc atggaacaca tatctttggt gaaatgttgg
     2761 ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cggttaataa caatggagtt
     2821 acagaacttt ttgccttgct gcatacagaa gaaggctgtt gctaatttcg ataggttctc
     2881 agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag ggttgccggg
     2941 caaccctcat tgaataaaac aggaattact tcgcttcgcc gtttttcatt tcgcccatag
     3001 ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt ttgatgatgt
     3061 aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc tggattgctt
     3121 caacgctcat cccgggcttg atgtaatcgc tcaggttgtc cagcaacagg acgcgcttct
     3181 ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg ttcttacgac
     3241 ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt tccattttta
     3301 tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt agggccaaca
     3361 ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca taccctttat
     3421 ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat ttgcgcaagg
     3481 cccggcaaat cagactcgat agcaactcaa gctgatgtgc cgtttccatg ttcagccaca
     3541 cataaccata gattggtgtt tccacaacct tcaggtcatg gtggttattc agcaactgac
     3601 gcagctcttc cacagcatcg atcaattttt ccgtattggc aaaaaccggc tgcggattac
     3661 cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc tgggtatcac
     3721 gaagtttttg cgcgttcaat aacacgaaat ggctggggcg cgtggcccag tatgcattga
     3781 tttgcaactc cagcatacaa accagatggc ggttaatggt ctggatacct tcatatatcg
     3841 attttggaat acgagtttct ttgctggcgg gcgcaatcag cccacgcatt ttcacggcat
     3901 cggtcaggag tttttgtaga tggctttcca gacgtgggcg ttcgagtaag ttcggtgaga
     3961 atgcagattg ataaacccga ttatactcgg tcagactttt cgccagttga atgcgccagt
     4021 ggatgaacgc ccgttgtggc cagataccgg taaacaacat tgccagtaaa gagccgagga
     4081 tcacatcgcc gcttcgccat aacgccgtat caatttcacc tgtcggggaa cccacaacaa
     4141 ttgccaacgt caccccaatc aataatcctt gatacggttt cttgcccagc gccagccaac
     4201 cgcaaagaaa catagccgcc gcgcaccaga ctaacatcag cggtaacgaa attaactcca
     4261 gttgcagagc gataagacct aaaatcgaac ccaacaccgt accgccaata cgttcgaagg
     4321 cgcgagggac aacgttaccc cagaacgaga ttggccccat aatcaccacc atggtgacca
     4381 gcggccaggt gctttccggg atagtaaaca ggcggataat gagaaaagtg agcaggaatg
     4441 ccagcgcgac ccgagtacca tgcacaatgc ggtaatggcg gtatacccgg atttcaaacg
     4501 ggcttaatga cttatcggca cgcacgcaga aactccgtaa tgaagattaa aagtgctcat
     4561 aactcatctg tcggccagaa ggcccgcctc tgaattatcc gacggacaaa ccgggaacag
     4621 tgtaatttcc cggcaggagc gaaaaaattg aaaggcgcat cttactcttt tcgctttcaa
     4681 aaaaagagtg gtcatcgcgt taacacaccg ccctgagatg aattagtgat gttttggctg
     4741 caccgcaaca tacatttcga tatcccagta cccatcttcc gcgccattgt tcaaataaac
     4801 ctcgaagcag ggctttggta acatttcata agcactgtcc tgcaagaggc tattaaagaa
     4861 ctgataccag ggtttagcaa aatcatcacc gactacacga gccaccgcaa ccgcatactg
     4921 accacctgaa atttctgtca gaatgacgcc ctcactgttt tcggggagcg taaagttatt
     4981 cggcaccgtc acgacgatgt cgcagcgtaa tttttcggcg ggtgtttcat ctggattgtc
     5041 gtaaaagaca gcaacccact ccttcggcac aatatttttg ctatctaccc acatcatcaa
     5101 ctgctcaaag cctttcttta ccgtctgttc ccacgggcca acgagatgga aacctgcaac
     5161 ggtacgttta tcttcctgct taatctcgta gttcatgacg tcccattaac actgttttta
     5221 tatacagtat aattgcgata ttaaaaccac aaggaatgag tgttgattat gcgagcagac
     5281 tcgcactcct gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact
     5341 caggcgagaa aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc
     5401 cacctgttta tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg
     5461 cgcggtgagc tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt
     5521 cggtagcacc atccagaact cttgttccgt tcccaggtcg atattttctt tatcgccata
     5581 ccagatgcgt tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt
     5641 ttgttgcccc caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc
     5701 ccccattacc actgcaatga gacgacgctg cccatcgaca gccgaagcaa tgagattaaa
     5761 cccggcacca gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa
     5821 taacccgtta cggttttgct gggtgatacc gttccaggtg agacttttct cactgtacat
     5881 atgataaaac tcgggctcgt cgtggatgat agcgcgagaa agcacagcta aatcataagc
     5941 cgagctatgc tgacctggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag
     6001 atgcagcttc tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat
     6061 atagtcagcc agtgcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa
     6121 atcacgtacc gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac
     6181 tggattatct ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg
     6241 atgactatcg atagtgcgat ccacgacata gcccgtcatc agctttgtca ggctggcggg
     6301 attgcgctgt tgatgctcat tacccgcggt gaggatctga ccggtggtgt aatccatcag
     6361 tacccaggag ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc
     6421 aaaaccagac gataagttaa aaatgaacaa agaagcagca ataataagac ggcgtttcaa
     6481 cagcaaaccc tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc
     6541 tggaataaag tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatctgttgt
     6601 cactcgctgt caaattgtcg cgctaaagct gattagcacg gtgatatttg atactctggc
     6661 agacagcaga aataacggat ttaacctaat gatgaatgac ggtaagcaac aatctacctt
     6721 tttgtttcac gattacgaaa cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt
     6781 cgcagccatt cgcaccgatg acgaattcaa tgtcatcggc gaacccgaag tcttttactg
     6841 caagcccgcg gatgactatt taccccagcc tggagcagta ttaattaccg gtattacccc
     6901 gcaggagaca cgagcgaaag gagaaaacga agccgcgttt gccgcccgta ttcactcgct
     6961 ttttaccgta ccgaagacct gtattctggg ctacaacaat gtgcgtttcg acgacgaagt
     7021 cacacgcaac gttttttatc gtaatttcta cgatccttac gcctggagct ggcagcatga
     7081 taactcgcgc tgggatttac tggatgttat gcgtgcctgt tatgccctgc gcccggaagg
     7141 aataaattgg cctgaaaatg atgacggtct accgagcttt cgccttgagc atttaaccaa
     7201 agcgaatggt attgaacata gcaacgccca cgatgcgatg gctgatgtgt acgccactat
     7261 tgcgatggca aaactggtaa aaacgcgtca gccacgcctg tttgattatc tctttaccca
     7321 tcgtaataaa cacaaactaa tggcgttgat tgatgttcct cagatgaaac ccctggttca
     7381 cgtttccgga atgtttggtg catggcgcgg caatacaagc tgggtggcac cgctggcgtg
     7441 gcatcctgaa aatcgcaatg ccgtaattat ggtggatttg gcaggagaca tttcgccatt
     7501 actggagctg gatagcgaca cattgcgcga gcgtttatat accgcaaaag ccgatcttgg
     7561 cgataacgcc gccgttccgg ttaagctggt acatatcaat aaatgtccgg tactggccca
     7621 ggcgaatacg ctacgcccgg aagatgccga ccgactggga attaatcgtc agcattgcct
     7681 cgataaccta aaaattctgc gtgaaaatcc gcaagtgcgt gaaaaagtgg tagcgatatt
     7741 cgtggaagcc gaaccgttta caccttcaga taatgtggat gcacagcttt ataacggctt
     7801 tttcagtgac gccgatcgtg cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt
     7861 accggcactg gatatcactt ttgttgataa acgaattgaa aagctgttgt tcaattatcg
     7921 ggcgcgcaac ttcccgggga cgctggatta tgccgagcag caacgctggc tggagcaccg
     7981 ccgccaggtc ttcacgccag agtttttaca aggctatgca gaagagattc agatgctggc
     8041 gcagcagtat gccgacgata aagaaaaagt ggcgctgtta aaagcgcttt ggcagtacgc
     8101 ggaagagatt gtctaatgtg aagcctccgc cgctggtacg gcggaggaca attttgagtc
     8161 aggctttttg aacggtgatg ctccacgctg catcgccaat ctgctgataa tcggtgatgg
     8221 catgtccttc ttctgccgcc cactgtggga ttgcttcagt tgcctgggtg caatcaaact
     8281 caattaccaa ctcatcgccg ctggccattt ctgccagtgc tgcttttgct tctatgagtg
     8341 gaaacggaca gacctgcgtc accacatcca gctttttaat cgccatcttt acaccttact
     8401 taatttgccg cagcagttgc cagtcgtgcc ttacgctgcg gtcgaacata cacaagccag
     8461 gacgcagtcc agactccgag aatcataaat acaaggccaa tccagccctg ccaggtcatc
     8521 attgcagtca tgaccagtcc attaccgata gagcaaccac ctgcaatgct ggcaccgaag
     8581 cccatcagta caccacctaa cccgctacgt aatgttgttt gtgcatcagc agcgcgaacg
     8641 cggaactcac ggctcgcttt ggctgcaata aatgacccca cgaagatccc taacaccagg
     8701 aaaacgcccc agttaatgta tttcacgtca cccgcgacca gaaattgcag aatattggcc
     8761 gttggggaag tgattcctaa cccaaacatg cgtccggttg cttcactcag gggccaagct
     8821 aaaagcgcaa tcaaaccgat gagtacagca gtgacaaagg gatgccagcg tttttcaaac
     8881 agaatatgag cgatcccggt tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc
     8941 ttcaactctt tcatcaccac ccagagcgtt atcaccagca aaacggcaac caacggccac
     9001 acagacaaat taaaagtatc agcaatagag ttatgttcag tagtgtagtg ctgcaaggtt
     9061 tgatttaaac cactggcgtg tggagaacgc atcaccgcac tcatcaccat ataagtgaaa
     9121 agcgcgatcc aactgccgat caatccctca cccgcgcgat accaggtccc ggtggcacat
     9181 ccacccgcca gaacaattcc cagcccgaaa agatacccac ctataacagt accaagccac
     9241 gggaacgccc cggcttcgta agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg
     9301 ctttgtaccg aaattgcaat cagcaaggcg taaaacatgc gattattttt cgcgatatac
     9361 atatcgcgaa aaccacctgt caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga
     9421 gcaccacaaa ttagcccgct taatatcatt gaaaacataa attaattaac cagatgaatg
     9481 ttaatgggga aattattcat gactggtgga atgcagacca ataaccaaat tctctaaatt
     9541 agaacaaatg gttattaatg aggatttcga taaaaaagaa taaaaaaacc ggagccaaat
     9601 gttcattcga ctccggttta ttattagaaa agatgattat gctacgtctt cgtactgcgg
     9661 aaccggatta cggaaactct tggttacgca agccaggtaa atcagaccga tagcagccca
     9721 gatcagaccc agaaccattg agctttcttc caggttaacc cacagcgcac caacggtcag
     9781 cgcaccacac atcggcagga acagatactg gaagtgatct ttcagcgtct tgttacgctt
     9841 ctcacggatc cagaactgcg aaataaccga caggttaacg aaggtgaacg ccaccagcgc
     9901 accaaagtta atcagcgccg tagccattac caggtcgaag ttgattgcca gcaaggcaat
     9961 cgcgccaacc aggatgatgt tcattgccgg agtacgccat ttcgggtgta cataaccgaa
    10021 gaagcttttc gggaacacac cgtcacgccc cattacgtac atcagacgcg ctacgcctgc
    10081 atgcgccgcc ataccggacg ccagtacggt aatggtggag aagatcagcg cgccaacctg
    10141 gaacgcttta cccgcaacgt acagcatgat ttcaggctgt gacgcatccg gatctttaaa
    10201 gcgagagata tccgggaagt acagctgcag gaagtaagtt gcaaagatga agatcatgcc
    10261 gccaatcagt gcggtcagga aaatcgcacg cgggatcacg cgctctgcat ctttggtttc
    10321 ttccgacagg ttgctgatgc cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc
    10381 ggtaatcatc gggataacat gcgcatcgcc agaccagaac ggacgagtgc tcgccagcgt
    10441 acccgcgcct tcaccttcaa atacgccata aacaaccatg cccagaatca ccgcgatcag
    10501 cactacctgc aacacgacga ttacagtgtt gaagttcgct acggatttca gactacgcag
    10561 gttaaaggcg gtcataaagg ccaccagcgc caccacaaac atccacgatg ggatggaagg
    10621 caccagagct tcaaaataga ttttcgccag cagaatgttg atcatcggcg cgaacagata
    10681 gtcgagcaga gaagaccaac ccaccataaa gccgacagtc gggctaatgg atttctgggc
    10741 gtaagtgtat gcagagccag cagaaggata gcggcgaacc agcttcccgt agctcagagc
    10801 cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc ggcacatgac catccgtgag
    10861 gcctgaaacg ataccaaatg tatcaaacag cgtcatcggc tgcatatagg caagacccat
    10921 cattacaacc ggaactaacg taagcgtttt acgcaattcc acgcgagagg tgtttggagt
    10981 aacgttatgc gacatggtca ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt
    11041 gccccatttt tttggattcc tcagcgacaa caactgtcga tttttagtaa atatctatcc
    11101 ggtacgaagc ccggcctctt ggtatgaatg attggtttga agcaaaaaat aaccgacgct
    11161 aattaaacgt cggtttttag tcattttttg acagcggcgc attgtgccta aatggggggg
    11221 aaatgacaag agaatgagag gcttgtcata ataatttttc tttaaatggc tgattttccg
    11281 tcatcagatt ttgcataaac accggcgatc gcactatttg ctaaaatttc gtcccgccac
    11341 caaccactgg caagccccgc ggtctgttca ttccagccaa tccagacggg ttcaaagagg
    11401 gtttgggcga ccactttctt ctcgattaac gcaccactat cgagaaaacg ttgcgccaga
    11461 taacggggta aatagccgca gccgaggccg ctaatttgca gctccagctt ggttttaaaa
    11521 tcgaaaacgg taatagcttc ttgttcgtcg agaagttgtg aggccgtaga agcgccggcc
    11581 tgtgcagtgt cccccaccac aatcgcccga tatcgtttaa tgatacgacg gtttaacggt
    11641 tcttcttcca gggctaacgg atgatgaggc gcgaccgcaa agacttgttc cagatcgccc
    11701 aaccgagaga aaccaaactc actggaagat ggtggctcat gcatcgcgcc aacaataata
    11761 tccgctcgcc cctgagtcag ggcatcccat gaaccaccga gtacaccatt gataaacttc
    11821 aggcgcgtca cgctatgatg ttgataaaaa gcctcaatga gtggcgcaag cagagaaaaa
    11881 gggaaggtgt cgtccacgcc aatcaccagc tcgttttccc agccttcatg cagtttgatc
    11941 gcctgctttt ccagttctcg cacggtatgc agaacttccc gccctttctc taataacatt
    12001 tttccggtgc gggtgaattt agcgcggtgt ccgctgcgat caagcaattg aatattaagg
    12061 tcgctttcca gcttgtgaac ggtgtaactc agagcggacg gcgtcttgta gagtttggcc
    12121 gacgccgccg caaaactgcc ttctttttct aaggcatcga ggatcatcaa cacatccagc
    12181 agtggtttca tacacgcccc cttgcagtgt atgcggcgtc ctgctggcgt gatcgtttac
    12241 tccagcggca ttaccagcgg atcgggatac tggtattcaa atcccagttc attacaaatc
    12301 cgactgccat caataatctt acctttgccg ctgtccagac tatttctgaa ctgcggaggt
    12361 tccagcccca ataaacgggc catctgcgga tagaaaacat tacgcgcagg gtgagctggc
    12421 gcacatatat tatagatgtg tccgccttta ggtgcctgta acaacagagt gatagcgcca
    12481 ataacatctt ctaaatggac taaattaaca ccatgttcac catcaggggc ggtttttccg
    12541 gcaaagaagc gtccgggatg acgtcccggt ccgaccaggc ccgcaagacg cagaatatcg
    12601 accgaagtac cgggtaaatt gtgcagccag tcttcgagtt cttctaatac tcgtccactg
    12661 ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca
    12721 gatgtcgagc tggtaaaaat aatgcgggga atacgatggg ccagcgcgct atccactaac
    12781 tcttgtaccg cttgtaaata gaactcatcg ccggggccgc tacgacgtgc cggaagcgta
    12841 atgaccagcg catcggcatc catcagggca tccagatcgt cagaatcgca aactaactca
    12901 ggctccatgc gaagcagata gctatcaatg ccactcattc gggccgcttc gacaccatct
    12961 tgtgtggttt tactcccggt gacttgccag cctcgcgctg aaagtgacat cgccagcggc
    13021 atgcctaacc accctaaccc gacaattgcg acctttttca tccgttatct cctgatacac
    13081 cttagatcta taaggctacg ctagcgtatc aaaactgaca attcattcta tgaatgaatc
    13141 tgttcaataa tgataacgac atgctgcaat gagcagtgaa tcatcagtaa ccgcgtatac
    13201 cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct gacaaattat gtttcagggg
    13261 ttctggtttc cccttacctt caaatggtgt tctgcgggta tctttgataa gttcattgat
    13321 ctttttaaca attcgcttat ctgtttcctg ccagtacaga taatcatccc atgattcctc
    13381 agaccagatt agtttcactc aataatgtcc ttttccgttc ctttgcctga tttcaggcta
    13441 tcgattgagt ccatcaatct ccgggcgtta gcgggggaac gcagtagata agccgtctct
    13501 tccagcgagt tgtattcttc gagtgacatc agaacacaag cctctccatt ctgacgagta
    13561 atgaggatcg gggcatgatc ttcaacggct ttcatcattg ttgccgacaa attctgacgc
    13621 gcttcgctgt agctaattgt acgcatgtaa atctcctctt ttgtacagtt cattgtacaa
    13681 tgatgggtgt taattcacta tttattaatt agtttgtaga tcaagacatt gccagcgaga
    13741 cgaaaaatca gactttgctc tttttggtgc tgtcaagtta gaggacagtc ctcttagccc
    13801 cctccttttc ccgctcattc attaaacaaa tccatcgcca taaaatatat aaaaaagccc
    13861 ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc agttgaataa acattcacag
    13921 agacttttat gacacgcgtt caatttaaac accatcatca ccatcatcct gactagtctt
    13981 tcaggcgatg tgtgctggaa gacattcaga tcttccagtg gtgcatgaac gcatgagaaa
    14041 gcccccggaa gatcaccttc cgggggcttt tttattgcgc ggttgttaac ggttcagaca
    14101 ggtttaaaga ggaataagaa aatgacagac aacactcgtt tacgcatagc tatgcagaaa
    14161 tccggccgtt taagtgatga ctcacgcgaa ttactggcgc gctgtggcat taaaatcaat
    14221 cttcacaccc agcgcctgat cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg
    14281 cgtgacgatg acattcccgg tctggtaatg gacggcgtgg tagaccttgg gattatcggc
    14341 gaaaacgtgc tggaagaaga gctgcttaac cgccgcgccc agggtgaaga tccgcgctac
    14401 tttaccttgc gtcgtctgga tttcggcggc tgccgccttt cgctggcaac gccggttgat
    14461 gaagcctggg acggtccgct ctccttaaat ggtaaacgta tcgccacctc ttatcctcac
    14521 ctgctcaagc gttatctcga ccagaaaggt atctctttta aatcctgctt actgaacggc
    14581 tctgtcgaag ttgcgccgcg tgccggactg gcggatgcga tttgcgatct ggtttccacc
    14641 ggtgccacgc tggaagctaa cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc
    14701 tgcctgatcc agcgcgatgg cgaaatggaa gaatccaaac agcaactgat cgacaaactg
    14761 ctgacccgta ttcagggtgt gatccaggcg cgcgaatcaa aatacatcat gatgcacgca
    14821 ccgaccgaac gtctggatga agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt
    14881 ctgccgctgg cgggtgacca acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg
    14941 ttctgggaaa ccatggaaaa actgaaagcg ctgggtgcca gttcaatcct ggtcctgccg
    15001 attgagaaga tgatggagtg atcgccatga gctttaacac aatcattgac tggaatagct
    15061 gtactgcgga gcaacaacgc cagctgttaa tgcgcccggc gatctccgct tctgaaagca
    15121 ttacccgcac cgttaacgat attctcgata acgtgaaagc acgcggcgat gacgccctgc
    15181 gggaatacag cgcgaagttt gataaaacca cggttaccgc gctgaaggtg tctgctgagg
    15241 agatcgccgc cgccagcgaa cgccttagcg acgagctaaa acaggcgatg gcggtggcag
    15301 taaaaaatat tgaaaccttc cacactgcgc aaaaactgcc gccggtagat gttgaaacac
    15361 agccaggcgt gcgttgccag caagtcacgc gcccggtagc ttcagttggg ttgtatattc
    15421 ctggcggctc cgccccactc ttctcaacgg tattaatgct ggcaactcct gcgcgtattg
    15481 cgggctgtaa aaaagtggtg ttgtgctcac cgccgccgat tgccgatgag atcctttatg
    15541 cggcgcagct gtgcggtgtg caggacgtgt ttaacgtcgg cggcgcacag gccattgccg
    15601 ctctggcgtt tggtacggaa tctgtgccga aagtggacaa aatcttcggg ccgggtaacg
    15661 cctttgtcac cgaagcgaaa cgtcaggtga gccagcgtca ggacggtgcg gcgatcgata
    15721 tgcccgcagg cccgtcggaa gtgctggtga ttgctgacag cggcgctacg ccagatttcg
    15781 tggcttctga tttgctttct caggctgaac acggcccgga ctcacaggtg attttactga
    15841 cgcccgctgc tgatatggcg cgtcgcgttg ccgaggccgt cgaacgccag ttggcagaac
    15901 tgccgcgtgc cgaaaccgcc cgccaggcac tgaacgccag ccgcctaatc gtgaccaacg
    15961 atttagcaca gtgcgtggag atctccaacc agtacggccc ggagcacctg atcattcaga
    16021 cccgcaacgc tcgcgatctg gtcgatagca tcaccagcgc cggttcggta tttcttggtg
    16081 actggtcacc ggaatcggca ggtgattacg cctccggcac caaccacgtt ctgccgactt
    16141 acggttacac cgccacctgt tccagcctcg ggctggcaga tttccagaag cgcatgaccg
    16201 tgcaggaact gtcgaaagaa ggcttttctg cgctggcttc aaccatagaa acactggccg
    16261 ccgccgagcg cctgaccgcc cataaaaatg ccgttacttt gcgtgttaac gcccttaagg
    16321 agcaagcatg agcaccgtga ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac
    16381 gccgtatcag tcggcgcgtc gtctgggcgg taacggcgac gtctggctga acgccaacga
    16441 atatcccgca gccgtggagt ttcagcttac tcaacaaacg ctcaaccgct acccggaatg
    16501 ccagccgaaa gcggtgattg aaaattacgc gcaatatgca ggcgtaaaac cggagcaggt
    16561 gctggtcagc cgtggcgcgg acgaaggtat tgaactgctg attcgcgctt tttgcgaacc
    16621 gggtaaagac gccatcctct actgcccgcc aacgtacggc atgtacagcg tcagcgccga
    16681 aacgattggc gtcgagtgtc gcacagtgcc gacgcaggac aactggcaac tggacttaca
    16741 gggcatttcc gacaagctgg acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc
    16801 gaccgggcaa ctgatcaatc cgcaggattt tcgcactctg ctggagctaa cccgcggaaa
    16861 agccattgtg gttgccgatg aagcctatat agagttttgt ccacaggcat cgctggctgg
    16921 ctggctggcg gaatatccgc acctggctat tttgcgcaca ctatcgaaag cttttgctct
    16981 ggcggggctt cgttgcggat ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa
    17041 agtgatcgcc ccctacccgc tctcgacgcc ggttgccgac attgcggccc aggcgttaag
    17101 cccgcagggg atcgtcgcca tgcgcgaacg ggtggcgcaa attattgcag aacgcgaata
    17161 cctgattgcc gcactgaaag agatcccctg cgtggagcag gtttttgact ctgaaaccaa
    17221 ctacattctg gcgcgcttta aagcctccag tgcagtgttt aaatctttgt gggatcaggg
    17281 cattatctta cgtgatcaga ataaacaacc ctctttaagc ggctgcctgc gaattaccgt
    17341 cggaacccgt gaagaaagcc agcgcgtcat tgacgcctta cgtgcggagc aagtttaatg
    17401 agtcagaagt atctttttat cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat
    17461 tttcaggtgg accgttttga caaactcgcc tttgaaccgg gcgtgatccc ggagctgctg
    17521 aagttgcaaa aagcgggcta caaactggtg atgatcacta atcaggatgg tctgggaaca
    17581 caaagtttcc cgcaggcgga tttcgatggc ccgcacaacc tgatgatgca gatcttcacc
    17641 tcgcaaggcg tacagtttga tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc
    17701 gactgccgta agccgaaagt aaaactggtg gagcgttatc tcgctgagca agcgatggat
    17761 cgtgccaata gttatgtgat tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg
    17821 ggtattaatg gtttacgcta cgaccgcgaa accctgaact ggccaatgat tggcgagcaa
    17881 ctcactaaac gagaccgtta cgcccatgta gtgcgtaaca ccaaagagac gcaaattgac
    17941 gtccaggtgt ggctggatcg cgaaggtgac agcaagatta acaccggcgt tggcttcttt
    18001 gatcacatgc tggatcagat cgccacccac ggcggtttcc gtatggaaat caacgtcaaa
    18061 ggcgacctct atatcgacga tcaccacaca gtcgaagata ccggcctggc gctgggtgaa
    18121 gcgttaaaaa ttgcccttgg cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg
    18181 atggacgaat gccttgcccg ctgcgcgctg gatatctctg gtcgcccgca cctggaatat
    18241 aaagccgagt ttacctacca gcgcgtgggc gatctcagca ccgaaatgat cgagcacttc
    18301 ttccgttcgc tctcttacac catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac
    18361 gatcatcacc gtgtagagag cctgttcaaa gcctttggtc gcaccctgcg ccaggccatc
    18421 cgcgtggaag gcgacaccct gccctcgtca aaaggagtgc tgtaatgaac gtggtgatcc
    18481 ttgataccgg ctgcgccaat ctgaactcgg tgaagtctgc cattgcgcgc cacggttatg
    18541 aacccaaagt cagccgtgac ccggacgtcg tgttgctggc cgataaactg tttttacccg
    18601 gcgtaggcac cgcacaagca gcgatggatc aggtgcgtga gcgcgaactg tttgatctca
    18661 tcaaagcctg tacccaaccg gtgctgggaa tctgcttagg gatgcaactg ctggggcggc
    18721 gcagcgaaga gagcaacggc gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa
    18781 tgaccgactt tgctctgcca ctgccacata tgggctggaa ccgcgtttat ccgcaggcag
    18841 gcaaccgcct gtttcaggga attgaagacg gcgcgtactt ttactttgtt cacagttacg
    18901 caatgccggt caatccgtgg accatcgccc agtgtaatta cggcgaaccg ttcaccgcgg
    18961 cggtacaaaa agataacttc tacggcgtgc agttccaccc ggagcgttct ggtgccgctg
    19021 gcgctaagtt gctgaaaaac ttcctggaga tgtgatgatt attccggcat tagatttaat
    19081 cgacggcact gtggtgcgtc tccatcaggg cgattacggc aaacagcgcg attatggtaa
    19141 cgacccgctg ccgcgattgc aggattacgc cgcgcagggt gcagaattgt tgcatctggt
    19201 ggatctgacc ggggcaaaag atccggctaa acgtcaaatc ccgctgatta aaaccctggt
    19261 ggcgggcgtt aacgtgccgg tgcaggttgg cggtggcgtg cgtagcgaag aagacgtggc
    19321 ggcgttactg gaagcaggcg ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc
    19381 agagatggtg aaaggctggt ttgaacgttt cggtgccgat gccttagtgc tggcgctgga
    19441 tgtccgtatt gacgagcaag gcaacaagca ggtggcagtc agcggctggc aagagaactc
    19501 gggcgtttca ctggaacaac tggtggaaag ctatctgccc gtcggcctga aacatgtgct
    19561 gtgtaccgat atctcgcgcg acggcacgct ggcaggttct aacgtctctt tatatgaaga
    19621 agtgtgcgcc agatatccgc aggtggcgtt tcagtcctcc ggcggtattg gcgacattga
    19681 tgatgtggcg gccctgcgtg gtactggcgt gcgcggcgta atagttggtc gtgcattact
    19741 ggaaggtaaa ttcaccgtga aggaggccat cgcatgctgg caaaacgcat aataccgtgt
    19801 ctcgacgtcc gtgatggtca ggtggtgaaa ggcgtacagt ttcgcaacca tgaaatcatt
    19861 ggcgatatcg tgccgctggc aaaacgctac gctgaagaag gtgcagacga actggtgttc
    19921 tacgatatca ccgcttccag cgatggccgt gtggtagata aaagctgggt atctcgcgtg
    19981 gcggaagtga tcgacattcc gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc
    20041 gcgaaaattc tttcctttgg cgcggataaa atttccatca actcccctgc gctggcagac
    20101 ccaacattaa ttactcgcct ggcggatcgt tttggcgtgc agtgtattgt ggtgggtatt
    20161 gatacctggt acgacgctga aaccggtaaa tatcatgtga atcaatatac cggcgatgaa
    20221 agccgcaccc gcgtcactca gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgc
    20281 ggtgccggag aaatcgtcct caacatgatg aatcaggacg gcgtgcgtaa cggttacgac
    20341 ctcgagcagc tgaaaaaagt gcgagaagtt tgccgcgtcc cgctgattgc ctccggtggc
    20401 gcgggcacca tggaacactt cctcgaagcc ttccgtgatg ccgacgttga cggcgcgctg
    20461 gcggcttccg tattccacaa acaaataatc aatattggtg aattaaaagc gtacctggca
    20521 acacagggcg tggagatcag gatatgttaa cagaacaaca acgtcgcgaa ctggactggg
    20581 aaaaaaccga cggactgatg ccggtgattg tgcaacacgc ggtatctggc gaagtgctaa
    20641 tgctgggcta tatgaacccg gaagccttag acaaaaccat cgaaagcggc aaagtcactt
    20701 tcttctcgcg cactaaacag cgactgtgga ccaaaggcga aacgtcgggc aatttcctca
    20761 acgtagtaaa tattgcgccg gactgcgaca acgacacgct actggtactg gcgaatccca
    20821 tcgggccaac ctgccacaaa ggcaccagca gctgcttcgg cgacaccgct caccagtggc
    20881 tgttcctgta tcaactggaa caattgctcg ccgaacgcaa atctgccgat ccggaaacct
    20941 cttacacagc caaactgtat gccagcggca ccaaacgcat tgcacagaaa gtgggcgaag
    21001 agggcgtgga aaccgcacta gcagcaacgg tacatgaccg ctttgaactc accaacgagg
    21061 cgtctgactt gatgtatcac ctgttggtgt tgttgcagga tcaggatctg gatttaacaa
    21121 cggtgattga gaacctgcgt aaacggcatc agtgagttgc ggggtaagcg gatgcgatgt
    21181 tgttgtcgca tccggcaaaa aaaacgggca aggtgtcacc accctgcccg ttttctttaa
    21241 aaccaaaaag attacttcgc gttgtaatta cgcagagcgt tacgccccag cacaatcccc
    21301 gcgccaacca tgccgcccag cagcaccgcc agaatcaaag tgattgcctt tttcgggcta
    21361 tcgcgacgaa taggtaacgt cggtttcatc acatagcggt aagcatgaat atcaagatca
    21421 tcaactttta agctttcgat atccagcagg ttttgacgtg tctgatagta gtttggtgag
    21481 aacaccaacg gacgagtcgc ttcatgctta atcatcgact ccagcgcttc gctccccaaa
    21541 aggaacaacg tatcttgtgt cacatcttca gtctgctgaa cctgtggctt tgtcacctgc
    21601 gcctgattcg catactgcaa cgcttcctga atctgacgga tacgcagatc tttctgctcc
    21661 tgcgcaacta cttcctgagt tttcagagaa tcctgcaagt ttttcatacg cagcgcgatg
    21721 ttgtctttca ggtcttttcc cagttcttca tttacctggt cgtcaacttg ctggatatac
    21781 ttcgccagct gcttctgagc accttcaggt gtttcaccaa cataagacac cgctaaaggt
    21841 aatgactgat ttttgacggt aggctcaatg gtcagctttt ctgcttcttc ctggttatcc
    21901 agcgtttccg ctaatgctga gaatgcagtg ctgtaacgtc cgataagtga agcctgaatg
    21961 tcggagactt tcggtacagc cgatccgtag atcacattca tcgcgttggt ataaccagcg
    22021 atttgcccca catcaggttg agtaacgata gctgtggaag tccacttttc tttggcaacg
    22081 gccagatacc ctatagccag ggcaatagca acaatgacag agatgataat tgtcatcttg
    22141 ccacgccaca actgcaccag taaatcaatc aaatcaatct gttccgggtc atggttttgc
    22201 ccagaaacat tattattttc tactctcatc attaccctaa cggacgaaat aattttaagt
    22261 cgccatagtt tagcgataat cgccatgaat gctataggat aaatgataaa aataatgaat
    22321 tattacaaag ggaacataag agaatcaggg cgaaaatcgc cctgataaca ggatgttagt
    22381 cactgccaaa gagatcgcgg gtgtagactt tgtctgccac atccttaagc tcttctgcca
    22441 tacggttgga gataattacg tcggcttgtt gtttgaaagt ggcgagatca cgttccagcc
    22501 gagagttgaa gaatgagtct tctttcatca ctggctcgta gatgattact tcaacacctt
    22561 tcgccttgat acgcttcata atcccctgaa tagaagacgc acggaagtta tctgaaccgc
    22621 tcttcataat caggcgataa atacccacca catgcggttt gcgtgacaaa atggcatcgg
    22681 caataaaatc tttacgcgtg cggtttgcat cgacaattgc cgagatcagg ttattcggca
    22741 cagactgata gttcgccagt aactgcttgg tatctttcgg cagacaataa ccaccataac
    22801 caaacgacgg attgttgtaa tggttgccaa tacgcggatc gagacaaacg ccttcgataa
    22861 tctggcgagt attcagacct aaactttctg cgtagctatc cagttcatta aagtacgcta
    22921 cgcgcatcgc cagataggta ttagcgaaaa gtttaatcgc ttctgcttcg gttgagtcgg
    22981 taaacagggt cgggatattt tgcttaatcg cgccttcctg taacagagca gcaaaacgtt
    23041 ctgcgcgttc tgaacgctca ccgatgacaa tacgtgaagg atggagatta tcgtaaaggg
    23101 ctttaccctc acggagaaat tccggagaga aaataatatt tttagtacga tatttcttat
    23161 gcatcgctgc ggtaaagcca acgggtaccg ttgatttgat gaccataacc gcataaggat
    23221 ttatctctac tacgtcctta attactgatt ctacactcga tgtattgaaa taattagtgt
    23281 taggatcata gtcggttgga gtggcgatga tgacataatc agcatcccgg taggcttcat
    23341 ttttatctaa tgtggcatta aagtgtattt tatctgattg caaaaactgc tgaatttcct
    23401 tatcaacaat aggagatatc cgatcattca gcatagcaac gcgtgacggt aaaatatcta
    23461 atgccacaac ctcatgattt tgtgcgatta gaagcccgtt tgacaagcct acatagccgg
    23521 taccggaaat ggtgattttc atttcatgct ctcagaatta acttaactgt gaatcatgat
    23581 gtttttagca tcctgataag agctaaaagt tttaacgcta cgttgttcga tggtcaacgc
    23641 aacgaaaaac aattcagatt ttctttataa gaatagctga tatttattaa gttaatatta
    23701 agcagtgaaa tttagtctat aagataattg gcaaaaaaaa agcccggcgt aatatacgcc
    23761 gggcctcaat ttattgttgg ttaaatcaga ttaatccagc cattcagtat ggaacacacc
    23821 ttctttatca atgcgcttat aagtatgcgc accaaaatag tcacgctgag cctggatcag
    23881 gttcgcaggc agaacagcgg cacggtagct gtcataatag gcaaccgcag cggcgaaggt
    23941 cggaaccggg ataccgttct gtactgcata agcaacgaca tcacgcagcg cctgctggta
    24001 gtcatcggca atttgcttga agtacggagc caacagcagg ttagcgatct gaggattttc
    24061 ggcataagca tcagttattt tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa
    24121 aatcttcgcg atttcgccgt agttcagatc ccagttgtac tcttcagacg ctgcgcgcag
    24181 ctgagagaag ccctgagcgt aagaaacgat tttgcccaga tacagcgcac ggcgaacttt
    24241 ttcgatgaac tcagccttgt cgccagctgg ctgcgcttgc gggccagaga gaactttaga
    24301 tgcggcaaca cgctgatctt tcagagaaga gatataacgt gcaaacacag actcggtaat
    24361 cagcgacagc ggttcaccga gatccagcgc gctctggctg gtccatttac cggtaccttt
    24421 gttagccgct tcatccagga tcacatcaac caggtagtta ccgtcttcat cttttttggt
    24481 gaagatgtct ttggtgatgt cgatcaggta gctactcagt tcaccgttat tccactcggt
    24541 aaaggtctgt gccagttctt cgttagagag attcaggccg cctttcagga gagaataggc
    24601 ttcagcaatc agttgcatat caccgtattc aataccgttg tgaaccatct tcacatagtg
    24661 acccgcgcca tcagcaccaa tataggtaac gcacggttcg ccatcttcag caacggcggc
    24721 gattttagtc aggatcggtg caaccagttc ataggcttct ttctgcccac caggcataat
    24781 ggaaggacct ttcagtgcgc cttcttcacc accggagaca ccggtaccaa tgaagttaaa
    24841 accttctgca gaaagctcac gattacgacg aatggtgtcc tggaagaagg tattaccacc
    24901 atcaatgatg atgtcacctt tatcgaggta tggcttaagg gaatcaatag cagcatccgt
    24961 gcctgcacct gctttcacca ttaacaggat gcgacgaggc gtttccagag attcaacaaa
    25021 ctctttcacc gtatagtaag gaaccagttt cttgcctgga ttttcggcaa tcacttcttc
    25081 cgtcttttca cgggaacggt tgaaaataga gacggtataa ccacggcttt cgatgttgag
    25141 cgcaaggttg cgccccatca ctgccatacc gactacgccg atctgttgct ttgacattgt
    25201 ttactcctgt caggggtggt cacgcggggt tacgcggctt gaagaagcta agatggtaac
    25261 ccagcatgta tagaataaga tagaagaata aacggtggtg tatcgcccta taaccactta
    25321 gaaaaagcct tagctttcag tgggtcaaat ggcccctcct ttatctctaa cataagggct
    25381 tttggtgaca ggcatttgac actatgtata tctcctgggg aaaattccac gacacttatt
    25441 cccgtaccgt ctccaacaac aaactttttt tggagctcac cattttgctc atacaaacaa
    25501 acttctaatt gcccttgcat gacgacaaac atttcccact gatgcggtaa ctcatgccaa
    25561 tggggttcaa catagctgtc gcgtacaaat gcaataagta aacgttgaac cttctcctga
    25621 tgagatgcgt gcaataaata atgagcgcgc aaacgttcag aatttgctgc agtgcgaaaa
    25681 agttcatcca taatattgtc atcaactaaa cgcattctcc tgcctccagt cggacttcga
    25741 tagtatgtgc cgccgattca accgaaaatt gagactttaa caacacattg gcgttctgac
    25801 ctaactgttt tcttaaaact gaatcactaa gaagcaattg tgcagattca aacagtttat
    25861 catcttcacc attaacatga atgaacccag ccctgtgctt attaattaca tccattagat
    25921 cattaccgcc attcacactc ccaaggatcg ggattgattg aaccatatac cctagtaatt
    25981 ttccggggaa attatgtgaa gaatggcgag acgaaaggga gaacaggcca acatcaactt
    26041 cagataaaat taatttaaac tcttcctggt tcactgaagg tagatgagtg aaattagtta
    26101 aattccattc tgcagaaaga gatttaatca ggtcaacttc atcaccctgc cctataaaca
    26161 ggaaatgagc atcatgatga cgcatcatat tacgcgcaag gcgcattaag tttgccatat
    26221 cctgagcatg cccaatatta ccgccataga aaaaaatgat tttatctttt agagcgtatt
    26281 tttgacgaag tgaatgataa tcatcgccgg cagacacagg agtcattgag gcccaattac
    26341 gtaaaacttc acacggataa cctttattgg cctgacgaaa tatctcaaga ttcttatcag
    26401 acattaaccc aatccagtca gcctgttgat atgatttttt ttcaaaatac ctgaaatatt
    26461 tttcaattgg cgagccggct tttatcatac cagcatcaat gacccactgt gggaacatat
    26521 cccttaggac cagatagctt gggcactggc atcgctcttt aattttttta accaagcctc
    26581 cccaaaaaat agagggggaa taataaacga taccatcaaa tgtatcatgt tggatgagat
    26641 gctttaatgc gtgccatgca cgaaaagata aaagagtttc atttatggca cgtttagcct
    26701 tacctatatc ctttaaaggt ccacttttga aacgccaaac ctttatacca tcagtcatgc
    26761 taacagaata gattgcctgt aatgtgttgt caggcgtaat tacagttaca tcatggcctc
    26821 tgctcagcaa ttccaggcct aactcatgaa acattttagc cccaactcgt gtgctatggg
    26881 gcaaataatc atcaatgatt aatgcaagtt tcattagtat tgcttccaga caacccgttt
    26941 aacgtagtca gtatatgaat ggataatacg cacaacttta tctgaaacat ttggcatact
    27001 atagtcactg acctgacgaa gtaagcgtac ttctccacga ggctgtgttg caataatttc
    27061 taatgcctgt aacacacggg cagactcaag accaaccatc attacagccc cttcttcgaa
    27121 gccttcaggg cgttcgtgcg cttctcgtat attgagcgca gggaagttca taatggagga
    27181 ctcctctgta atagtcccgc tatccgataa aacagcacgt gcattttttt gcagatggtt
    27241 gtaatcgtga aatcctaatg gcttaagtag caagatattt ttatgaaatt gaataccgtt
    27301 ttcgttgatg cggttacgag tgcgcggatg agtagaaaca actaccggga tatcatattt
    27361 ttcagccacg gtattaagta tatttgccag tttcgcaagc tgtttagggg tatcaacatt
    27421 ttcttctctg tgggcactta ccacaaagaa attcccaggt gttaaattca ggcgagaaag
    27481 tacatcggaa ccatcaatct gcgacatata atgagtgagt acttcaaaca ttgggctacc
    27541 ggttttaata atcctatcgg ctggtacacc ttcagccaaa agatattcac gcgcgatatc
    27601 actatatgtc atattgatat cagcggtgtg gtcaactatt tttctgttag tttcttccgg
    27661 tacgcgttga tcaaaacaac gattcccagc ctccatatgg aaaatcggaa ttcttcgacg
    27721 ctttgctggt attgctgaaa tacaggagtt agtatcgcca agaactaaca tggcttctgg
    27781 tttttcctgt tcaaagacct catcaacttt aatgataact tgtccaatag tctctgctgc
    27841 atttttacct gcggcattaa gaaaataatc aggtttgcga acacccaaat ccttgaaaaa
    27901 aacctcattc aattcataat cgtaattttg cccagtatga acaataaggt gatcacaata
    27961 ttcatctaat tttgcaagga cacgcgagag tcgaataatt tctggacgag tcccaacaac
    28021 cgacatgact ttcaattttt tcacgataaa actctcgcta tagtatctgg ttcagaacga
    28081 ttaaatattt catttgccca aagcataaca actagctcat ccgagccatt attagtaatg
    28141 tcatgcgtcc atcccggaac tgtttcaaca attttaaaat catctgatga aacattaagt
    28201 tcatatcgtt cacccgtgac aatattttca aatttgaaac aagcacttcc tcggatgata
    28261 ataaatttct catttttgga atgatgataa tgcccccccc gagtaattcc tggatgcgca
    28321 gtaaaaaacg aaaactggcc cgcgtttttc gttttcaata cttcacagaa tacccctctg
    28381 tcatcactat aagaaggaac cgtatacgca aactgtttag gagaaaggta acttaaccat
    28441 gttgagtaca atgctcgagt gaatccatta cctacatctt cgatgattaa tgtttggcga
    28501 ctttctttaa atcgataaat taattgtgcc acctcaccaa cagtaacaga ataaattgga
    28561 ccaaatgtcc tgtaaccagt ttcgttcgct ccttctaata gctttaatat gtcagaacaa
    28621 aagtcatcta tatacaccaa atttacaact gctgaaggat cattaattgt aatagcttca
    28681 tcatttgcaa tgcgatggca gaaagttgct ataaaggagt tataatttgg ttgacaccac
    28741 ttaccgaata cattcggcaa gcgataaata taatatttag cgtttgtcgt ttccccatac
    28801 tgctgaatga ttttttccgc aactgcttta ctctttccat aagcgttatc acattcagct
    28861 tggatggatg aactaagcat gataggagta tctttattat tttttttcag aatatcaaca
    28921 atctgttgag ttagatcgca attcccctct tcaaactcac tctccttcac aggacgattt
    28981 actccagcaa gatgaaaaat gaaatctgcc tgcttaagtc cctgctctaa atccgtcaaa
    29041 gaagagttac gatctatcgc aataagttcg ttatatccag cttccttaag gcggaaaacc
    29101 aaatttcgac cgataaaccc tgcagcgcca gtaactaaaa ttttcatatt atgaatccaa
    29161 ctcataatct tcaccagaac gaagtgcccg gataaaagga agttttagca gtaattcttt
    29221 cattccctca acatctaatc tctcagtatt atgagaatta taatcttcca cttccgagat
    29281 acgacggtca ccatgttcca catattttcc atagttcaaa tcgcggagat ctggtggaac
    29341 acgataataa tcacccatat cttccgctgc aatcatttcc tctcggctca ataacgcttc
    29401 gtaaagtttt tccccgtgtc gagttccaat aatattgatt ggatgctcat gggcatttag
    29461 caattcctta agtgcaatgg ctaatgtttg aattgttgcc gcaggtgctt tctgaacgaa
    29521 aatgtcacca ttatttccat gttcgaaagc ataaaggacc agatcgacag catcctcaag
    29581 cgtcatcatg aaacgagtca tattgggatc ggttatggtc aatggtttac cagctttgat
    29641 tagatcaaca aataatggga tgaccgatcc acgtgaagcc attacatttc cataacgagt
    29701 tccacagata actgtttttg aactgtcaag attacgtgat tttgcaacaa taactttttc
    29761 catcattgcc ttagatatgc ccattgcatt gataggataa acggctttat cggtactcaa
    29821 gcacaccacg cgtttaatcc cattcgcaat agcagcctcc agaacatttt ccgtacccag
    29881 aacatttgtc ttaaccgcct ccatagggtg aaattcacat gaaggaactt gtttaagggc
    29941 tgctgcatga tacagaaaat cggcaccacg cgttgcattt agaacggaat tatagtctcg
    30001 cacatcacct atataaaatt ttaattttga gttattatat tttttccgca tatcatcttg
    30061 ttttttttca tccctgctaa atattcgtat ttcagtaata tctgtatcta gaaaacgctt
    30121 aagtacggca ttaccaaaag aaccagtgcc accagtgata acgagtgttt tattcttaaa
    30181 catattttca ccttttacag atatttttta tagtcgtaat atgagctttt catatagcca
    30241 tattttgttt tagaagcaac tttcgccaaa gttgcacttt cttcatgatg gacaatagca
    30301 tcaggctcat agaaaatctt cccatttact tccattaatt ggccagccaa atatgcttcc
    30361 tcaccatata aaaaaacttt gttatctaat tcatcaaaat atttgaagaa ggaaggcata
    30421 aggacataac aggctccaat acccatatgt atataacctc taccctgatc aaaggaatta
    30481 tttcttccac ctttcaaata atttaaaaac ctgcttcccc acgttaaaac taaagcagcg
    30541 atataatttg aaaaatatat atcatacaaa aatttcctaa acttacttac tcgcttacgg
    30601 cagtgtgggt tctggtgata accatccttt gtaatcacat ttggagcaat aactaaagca
    30661 tcgtccggat atgtagtctg tgatattatt tgcgtaaaat tactctcata aaccaaatca
    30721 ttattcccaa caactattgg atgatttctt ggtagtcctt ttaggccaag attcaatccg
    30781 ccaaaatatc ccaaattcac atcactttta attaatctga ccttctcgtt atatttactc
    30841 tcaatatcat ttaacttttt tagctcttgc tcattagaac agttatctac aacaattaca
    30901 aagctatctt tttcttgatt aataacgctc
//
LOCUS       DAZFKM010000049        30857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFKM010000049 DAZFKM010000000
VERSION     DAZFKM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(91..2124)
                     /gene="betT"
                     /locus_tag="ACHKAY_002308"
     CDS             complement(91..2124)
                     /gene="betT"
                     /locus_tag="ACHKAY_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO5700977.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2253..2840
                     /gene="betI"
                     /locus_tag="ACHKAY_002309"
     CDS             2253..2840
                     /gene="betI"
                     /locus_tag="ACHKAY_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO5700978.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2854..4326
                     /gene="betB"
                     /locus_tag="ACHKAY_002310"
     CDS             2854..4326
                     /gene="betB"
                     /locus_tag="ACHKAY_002310"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO5700979.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4340..6010
                     /gene="betA"
                     /locus_tag="ACHKAY_002311"
     CDS             4340..6010
                     /gene="betA"
                     /locus_tag="ACHKAY_002311"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO5700980.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6223..6888
                     /gene="ykgH"
                     /locus_tag="ACHKAY_002312"
     CDS             6223..6888
                     /gene="ykgH"
                     /locus_tag="ACHKAY_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO5700981.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVILFIIAICMAMMLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(7134..7829)
                     /gene="ykgG"
                     /locus_tag="ACHKAY_002313"
     CDS             complement(7134..7829)
                     /gene="ykgG"
                     /locus_tag="ACHKAY_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO5700982.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7822..9249)
                     /gene="ykgF"
                     /locus_tag="ACHKAY_002314"
     CDS             complement(7822..9249)
                     /gene="ykgF"
                     /locus_tag="ACHKAY_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO5700983.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9260..9979)
                     /gene="ykgE"
                     /locus_tag="ACHKAY_002315"
     CDS             complement(9260..9979)
                     /gene="ykgE"
                     /locus_tag="ACHKAY_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO5700984.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10506..11360)
                     /gene="rclR"
                     /locus_tag="ACHKAY_002316"
     CDS             complement(10506..11360)
                     /gene="rclR"
                     /locus_tag="ACHKAY_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO5700985.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11586..12911
                     /gene="rclA"
                     /locus_tag="ACHKAY_002317"
     CDS             11586..12911
                     /gene="rclA"
                     /locus_tag="ACHKAY_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO5700986.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13020..13256
                     /gene="rclB"
                     /locus_tag="ACHKAY_002318"
     CDS             13020..13256
                     /gene="rclB"
                     /locus_tag="ACHKAY_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO5700987.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13268..13861
                     /gene="rclC"
                     /locus_tag="ACHKAY_002319"
     CDS             13268..13861
                     /gene="rclC"
                     /locus_tag="ACHKAY_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO5700988.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14021..14890)
                     /locus_tag="ACHKAY_002320"
     CDS             complement(14021..14890)
                     /locus_tag="ACHKAY_002320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO5700989.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            15139..15996
                     /locus_tag="ACHKAY_002321"
     CDS             15139..15996
                     /locus_tag="ACHKAY_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5700990.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(16117..20370)
                     /gene="fdeC"
                     /locus_tag="ACHKAY_002322"
     CDS             complement(16117..20370)
                     /gene="fdeC"
                     /locus_tag="ACHKAY_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO5700991.1"
                     /translation="MSHYKTGHKQPRFRYSVLTRCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTKLTFTV
                     KDAYGNLVTGLKPDAPQFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     NADASTGQANLQVDTAAQKVANGKDAFTLTANVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            20922..21011
                     /locus_tag="ACHKAY_002323"
                     /pseudo
     CDS             20922..21011
                     /locus_tag="ACHKAY_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21485..21586
                     /gene="ykgR"
                     /locus_tag="ACHKAY_002324"
     CDS             21485..21586
                     /gene="ykgR"
                     /locus_tag="ACHKAY_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO5700992.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            21950..22213
                     /gene="ykgM"
                     /locus_tag="ACHKAY_002325"
     CDS             21950..22213
                     /gene="ykgM"
                     /locus_tag="ACHKAY_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5700993.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            22213..22353
                     /gene="ykgO"
                     /locus_tag="ACHKAY_002326"
     CDS             22213..22353
                     /gene="ykgO"
                     /locus_tag="ACHKAY_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO5700994.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(22388..22615)
                     /gene="ykgL"
                     /locus_tag="ACHKAY_002327"
     CDS             complement(22388..22615)
                     /gene="ykgL"
                     /locus_tag="ACHKAY_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO5700995.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            23437..23979
                     /gene="ecpR"
                     /locus_tag="ACHKAY_002328"
                     /gene_synonym="matA"
     CDS             23437..23979
                     /gene="ecpR"
                     /locus_tag="ACHKAY_002328"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO5700996.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            24053..24640
                     /gene="ecpA"
                     /locus_tag="ACHKAY_002329"
                     /gene_synonym="matB"
     CDS             24053..24640
                     /gene="ecpA"
                     /locus_tag="ACHKAY_002329"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO5700997.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            24698..25366
                     /gene="ecpB"
                     /locus_tag="ACHKAY_002330"
                     /gene_synonym="yagY"
     CDS             24698..25366
                     /gene="ecpB"
                     /locus_tag="ACHKAY_002330"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO5700998.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            25392..27917
                     /gene="ecpC"
                     /locus_tag="ACHKAY_002331"
                     /gene_synonym="yagX"
     CDS             25392..27917
                     /gene="ecpC"
                     /locus_tag="ACHKAY_002331"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO5700999.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            27907..29550
                     /gene="ecpD"
                     /locus_tag="ACHKAY_002332"
     CDS             27907..29550
                     /gene="ecpD"
                     /locus_tag="ACHKAY_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO5701000.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            29519..30229
                     /gene="ecpE"
                     /locus_tag="ACHKAY_002333"
     CDS             29519..30229
                     /gene="ecpE"
                     /locus_tag="ACHKAY_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO5701001.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            30542..>30857
                     /gene="ykgJ"
                     /locus_tag="ACHKAY_002334"
     CDS             30542..>30857
                     /gene="ykgJ"
                     /locus_tag="ACHKAY_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO5701002.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYG"
BASE COUNT         8032 a         7842 c         7329 g         7654 t
ORIGIN      
        1 caatcatata taatatatgc ataatttttt acagcgagtc aatgattccc gcgccatatg
       61 cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca cgctatggcc
      121 cggcgcttca cgatggagat gaataaagtt aaggtgccgc tcgtactggt caagaatatc
      181 ggtgatcacc tgctctttgc tgtagtccat caggtcgttg ccctggctgc cttctaacag
      241 gaaggtttcc agccggtagt aggtcgattt accgctacgt gcgcggtagg taaagcccgg
      301 caccgaatat tgctgcggcc aaatctgata gacaaagttt tgttcttcgc ccatatgcac
      361 caacaaatcc agatgcccca actgttgtcc ctcttccggc ggcaggcttt ttagctccac
      421 gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc attgccgggt aacagaccgt
      481 ctccatcatc tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg agagacgttt
      541 tttccagctc aggcgatcct gaagccccag cggtcgcggt gcggtatcgc ggttggcact
      601 ttcacggcgg taatcttcta ccttcagaga tttatacaac cccgccatca cgaagaagat
      661 cacaaagctg aacggcagcc ccataatcac cgtggtgttt tgcagcgcgg atatcccgtt
      721 ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc gaccagaaga cgcgcagcca
      781 gccgggggcg tcgctgttga tatctttaag ctgcgaggtg aaattcccca gcaccagcgc
      841 accggagtcc gccgaggtca cataaaacag caggccagta atggtggcga cggaggcgct
      901 aaaggtaaac gccggatact gcgccagcag gctgtagaag ccgcgctccg gatggaccat
      961 cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc tattgccgaa
     1021 caccgagagc cataacagcg tgaaggtaaa cggaataatc aacgtgccca gcacgaactg
     1081 gcgaatggta cgcccacgcg agatacgcgc caggaacaag ccgacaaacg gcgaccatgc
     1141 cacccaccat gcccagaaga agagcgtcca gttattcatc cactcaactg gacggtcgaa
     1201 ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa cattcagcac
     1261 cagcgcatta agcaggaacg aggtgtcgcc cataaacaat acgaacagga tcaatcccag
     1321 cgccagcgca acgttaagtt ccgataacac acgaatcccc ttatcgacac cggaggtgac
     1381 agagatcgtg gcgattatca ccgacaaggc gatcagtgcc gcttttgccg ccatcgaatc
     1441 gggaatatca aacagtacgc tcaagccata gttaagctgc accacaccga taccgagcgt
     1501 agtggcaata ccgaagatgg tgccgatcac cgctgcaata tccactgagt gccctatcgg
     1561 cccgttaatc cgtttaccga agatcgggta cagcgccgag cggatggtga gcggcaaatt
     1621 ataacgatag ctaaagtatc cgagcgccat gcccatcagc gcatacatcg accagccggt
     1681 taagccgtag tgaaacagcg tccagaccat cgcctgacgc gcggcctcaa tcgtctgtcc
     1741 cgcgccttcc ggcggctgca tatactgcgt taccggttcg gctacggaga agaacatcag
     1801 gtcgataccg atcccggcag caaacagcat cgccgcccaa ctcagcaggc tgaattccgg
     1861 tttggattgt tctggcccga gcttcaccga accaaaacgc gaacaagcga tacagaccac
     1921 aaagacaata tagagcgttg ccgccagtag atagtaccaa ccgaaggttt tagaaaccca
     1981 gtccagcgtg cggccaatcc acagggccga gaagtcgcga aacaggatcg ttgtcaggga
     2041 aaacaacaaa atcagtccgg cggaggtgta aaacaccacc ggattgattt tgtccttttc
     2101 cctgctgtgt gaaaggtctg tcatccagta tccccactgt tattgttacc atttaaaatc
     2161 aaattcgtaa caattaagac acattttata ttgaacgtcc aatcaataac cgctttaata
     2221 gataaacaac gctgatgaat ggagtggcga aaatgcccaa attggggatg cagtcgatcc
     2281 ggcgcagaca actgatcgac gccacactgg aagcaataaa tgaagtgggc atgcacgatg
     2341 caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc agccactatt
     2401 tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga tatcaccagt cagctgcgtg
     2461 acgcggtttt gaatcgatta catgcacttc cgcagggcag tgcagagcag cgattacagg
     2521 cgattgttgg cggaaacttc gatgaaacgc aggtgagcag tgcggcgatg aaagcctggc
     2581 tggcgttctg ggccagcagt atgcatcagc cgatgctcta tcgtttacag caggtcagta
     2641 gccgccgctt attgtcgaat ctggtgagcg aatttcgccg cgagttaccg cgccaacagg
     2701 cacaggaagc gggctacggc cttgccgcgc tgattgacgg attatggctg cgcgcggcac
     2761 tgagcggcaa accgctggat aaacctctcg cacactcgct gacccgtcac tttattactc
     2821 agcatttacc caccgattaa ccgaggagac gtgatgtccc gaatggcaga acagcagctt
     2881 tatatacatg gtggttatac ctccgccacc agcggtcgca ccttcgagac cattaacccg
     2941 gccaacggta acgtgctggc gaccgtgcag gccgccgggc gtgaggatgt cgatcgcgcc
     3001 gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat ggagcgctcg
     3061 cgtattctgc gtcgggccgt tgatattctg cgtgaacgca atgacgaact cgcaaaactg
     3121 gaaaccctcg ataccggaaa agcatattcg gaaacctcaa ccgtcgatat cgttaccggt
     3181 gcggacgtgc tggagtacta cgccgggctg atcccggcgc tggaaggcag ccagatcccg
     3241 ttgcgtgaga cgtcatttgt ttatacccgc cgcgaaccgc tgggtgtggt ggcggggatt
     3301 ggcgcatgga actacccgat ccagattgcc ctgtggaaat ccgccccggc gctggcggca
     3361 ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt aaagctggct
     3421 gaaatttaca gcgaagcggg cctgccggac ggcgtattta acgtgttgcc gggggggggc
     3481 gcggagaccg ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc atttaccggc
     3541 ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg cctcttccct gaaagaagtg
     3601 accatggaac tgggcggtaa atcaccgctg atcgttttcg acgatgcgga tctcgatctc
     3661 gccgccgata tcgccatgat ggcgaacttc ttcagctccg gtcaggtgtg taccaacggc
     3721 acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat tctggcgcgc
     3781 gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcag cccgctggtc
     3841 agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga ggaaggcgcg
     3901 cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct tcgataacgg cgcatgggtt
     3961 gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga agagatcttc
     4021 ggtccggtga tgtcgcttct cacttatgaa tctgaggacg aagtgattcg ccgcgccaat
     4081 gataccgact acggcctggc ggcgggcatc gtgacggcgg acctgaaccg cgcgcatcgc
     4141 gtcattcatc agctggaagc gggtatttgc tggatcaaca cctggggcga atccccggca
     4201 gagatgcccg ttggcggcta caaacactcc ggcattggtc gcgagaacgg cgtgatgacg
     4261 ctccagagtt acacccaggt gaagtccatc caggttgaga tggctaaatt ccagtccata
     4321 ttctaaccag gaggtttatt tgcaatttga ctacatcatt attggtgccg gctcagccgg
     4381 caacgttctc gctacccgtc tgactgaaga tccgaatacc tccgtgctgc tgcttgaagc
     4441 gggcggcccg gactatcgct ttgacttccg cacccagatg cccgcagcgc tggcgttccc
     4501 actacagggc aaacgctaca actgggccta tgaaacagag cctgaaccgt ttatgaataa
     4561 ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga tcaacggcat
     4621 gtgctacatc cgtggcaatg cgctggatct cgataactgg gcgcaagaac ccggtctgga
     4681 gaactggagt tatctcgact gcctgcccta ctaccgcaag gccgagactc gcgatgtggg
     4741 cgagaacgac taccacggtg gcgatggccc ggtgagcgtc actacctcca aacccggcgt
     4801 caatccgctg tttgaagcga tgattgaagc gggcgtgcag gcgggctacc cgcgcacgga
     4861 cgatctcaac ggttatcagc aggaaggttt tggtccgatg gatcgcaccg tcacgccgca
     4921 gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc gccctaacct
     4981 gaccattcgt attcacgcta tgaccgatca catcattttt gacggcaaac gcgcggtggg
     5041 cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc gcaacggcca acaaagaagt
     5101 gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg caacgctccg gcgtcggcaa
     5161 cgctgaactg ctggcggagt ttgatattcc gctggtgcat gaattacccg gcgtcggcga
     5221 aaatcttcag gatcatctgg agatgtatct gcaatatgag tgcaaagaac cggtttccct
     5281 ctaccctgcc ctgcagtggt ggaaccagcc gaaaatcggt gcggagtggc tgtttggcgg
     5341 cactggcgtt ggtgccagca accactttga agcaggtgga tttattcgca gccgtgagga
     5401 atttgcgtgg ccgaatattc agtaccattt cctgccagta gcgattaact ataacggctc
     5461 gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc tcaatgcgct cgccaagccg
     5521 tgggcatgtg cggattaaat cccgcgaccc gcaccagcat ccggcgattc tgtttaacta
     5581 catgtcgcac gagcaggact ggcaggagtt ccgcgacgca attcgcatca cccgcgagat
     5641 catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa atcagccccg gcacggaatg
     5701 tcagacggat gaacagctcg atgagttcgt gcgtaaccac gccgaaaccg ccttccatcc
     5761 gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg aaggccgcgt
     5821 acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt atgccgcaga ttatcaccgg
     5881 gaatttgaac gccacgacaa ttatgattgg cgagaaaata gcggatatga ttcgtggaca
     5941 ggaagcgctg ccgaggagca cggcgggata ttttgtggca aatgggatgc cggtgagagc
     6001 gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat cagattgaga gttaccgttc
     6061 cagagagggg gaccgaatcc ttatataaac accgaggtaa ctctcatgct tcatatccag
     6121 tatccatgat gctggctttt tgctgatatt taacccgaac attcctgata cagactatta
     6181 atacaacctt atctgcgaca gattttattt tctggatata ttatgcgtga acaaatcaaa
     6241 caggatatcg atctgattga gattttattt tatctgaaga aaaaggttcg tgttatcctt
     6301 tttattatag ctatctgtat ggcaatgatg cttttatttc tgtatatcaa taaagataat
     6361 ataaaagtga gttacagcct aaaaataaac cagacaacac caggcatact tgttaactgt
     6421 gatagcaata ataactttgc ctgtcagacc acaatgactg aagatgttat acagcgaatt
     6481 actacatttt ttcacaccag cccagatgtc aagaacagag aaataaagct ggaatggtca
     6541 ggagataaga gagatttacc aactgctgaa gcggaaatat ctcgcgtgca ggcgtctatt
     6601 atcaaatggt atgcgtcaga ataccataat ggcaggcaag ttctcgatga gatacaaaca
     6661 ccttcagcaa ttaacagtga gctttataca aaaatgatat acctgaccag gaactggtca
     6721 ttgtatccga acggtgatgg ctgtgtaact ataagctcac cagaaataaa aaataaatac
     6781 cctgctgcca tttgcctggc tctgggattt tttctaagca ttgtaatttc tgtaatgttt
     6841 tgccttgtca aaaaaatggt agatgaatac caacaaaact ctgggtagta ataaacagca
     6901 ttctgataga gagtgcctgt ataaagctga agtgacaatg accgggaagg gaacccgttt
     6961 tcaggaatgg atgcctgtct ttttcattgt aaatatcctg gcattactaa tgccaggata
     7021 tttacagaag agcagcataa ccatgacgga acggggttaa gtcaggttgt ccttaacgct
     7081 attttcaacc tcgtatgcct tcttcaggtt tatgttcaga tctcatatct ctctcagcaa
     7141 tcctcaataa tcagatacac cgctttcacc ggaccatgaa ccccgacgac tttgataagc
     7201 tcaatatccg ccgttgaact agggccgcta atgatgttaa tgcaggacgg cattcgttca
     7261 ccggcctgcg ctttctgatg caatttttct gcgagttgcg ctacacgcgg caagatagtg
     7321 cttttacgca ggataaaaag agaagattcc gggagcaggc tcaatgaacg cccgcgcccg
     7381 gcggcggaaa aaagaaccac gcctcccgat tcggttaaac catattcagc atacacgaca
     7441 cccactttag cctgctcggc ctgcgagata ttctcggcac ctttcgccgg atcccaaaca
     7501 acggcattgc attcctgctg caaacgttca ctaatcccca attcctccag cctcgtgtca
     7561 ccgctaatca cgaccgactg atctcccagc tctttacaca gacgtattgc agcttctgcc
     7621 gccttcgcct cgctggtcag ctcacagcgc gtcaacataa catcgctggc aaactgaata
     7681 aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc gctcgttagc atagttgtta
     7741 agcggcgcat cttctgcttg cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg
     7801 ttattcaaaa attcgcctcg attatccatt ctttttctcc tgcgcctgat gtttcttaaa
     7861 ccaactacgg aaactctctc cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct
     7921 aatcgcgcca aatttgagtg gtgttttgcc gccattgata aaccagcttg ccgcatgagc
     7981 gccggccatc atcccgactt tccacaatcc tggatgacta ttggcataag cgaacatttt
     8041 tatcgcccgt tgctctgctt ttgcggtgat acctttttca gccatcaccc gacgatgacg
     8101 caaaatcagt tttgacagcg gaatacgcac cggacacacg ctgtcacaag cagtgcataa
     8161 agagcaggcg tagggtaaat ctttaaaatc tttatagccg ccaagtagcg gagaaatcac
     8221 cgcaccaatt ggccctggat aaatagagcc atatccatga ccgccaatat ggcgatatgc
     8281 cggacaagta ttcatacaag ccccgcagcg aatacagcgc agcacatccc gaaattcaga
     8341 ggccagcacc tcagaacgcc cgttatcgac aataaccaga tgaaactctt caggaccatc
     8401 aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg tatcccgtca aacgtgcacc
     8461 aacggcactg cgcgccagca tggtgatcaa tacatctacc tcggcaaacg tgggggcaat
     8521 acgctccatt cccatcactg caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt
     8581 accttcattg gtcaccaggc ataccgaacc tgtctctgcc accgcgaaat tacagccggt
     8641 aatacctatt tcagcactga ggaaatcttc gcggattttt tgccggatga ataaggtcat
     8701 cgcttcaggc gtttccggcc cctcatagcc cagacgttcg tgtagcactc gacggatctg
     8761 atggcgatct ttatgaattg ccgggaccac aacatgagat ggcggatctt gatccagctg
     8821 gagaatgtat tcacccagat cggtttcaat cacctgaatg ccagcatcct gcaacacatg
     8881 attgacacca atctcttcgg tcaccatcga tttagatttc accaccttcc gggcattttt
     8941 gcgttgggca acctgtaaaa tgtagcgagt ggcgtcttct ttagtttttg caaaatagac
     9001 gtgaccgccg ttttgcgtca ctttttctga gagctggtac agataagcgt cgagattact
     9061 tagaacatga tcacgtatct gggcggcccg atcgcgccac tcctcccagt gccccaattc
     9121 atcgaccatt ttttgccgat ttgccccaat acgctgctgc gcgtttgcca ccgctttgcg
     9181 catgatcgga tcttcaattt gctgacggat gcgtgtctta aaatctgtat tactggtttt
     9241 gatcgacatc tttatatcct cagcggctca tcaacacttc agcaatatgc atcactttga
     9301 ctttctgccc ttcccgttgt aatcgcccac tgatgttcag caggcaactc acgtcagcac
     9361 caattaaata ctcagggcgg acttccatca ggtgcacaac cttttctttc accatctcgc
     9421 cggatatttc ggccattttg accgagaacg tgccgccaaa tccgcagcag gtatcctgtt
     9481 cagcaaaggt caacagctcc agtccacgca catttttcag cagcgtaagt ggctcgtcct
     9541 tcactcccag cttacgggcc aggctacaag atgggtgata cacagctctc ccttgcaaac
     9601 tggcacctac atcgactacc cctaatttat taacaataaa agaggtgaga tcctgcatac
     9661 gcgcggcaac cttttcggca cgtgatgccc attcaggttc atccgccaga tacgtcgggt
     9721 aactttttac ggcataggtg caagagccag ccggtgaaat aatgggatcg tcgttatcct
     9781 ccagtgcggc gatcagattt ttcatccctg gaatcgcttc tttgatataa ccgctattga
     9841 tcgcaggctg accgcagcat ccctgtttct ccgggaaatt tacgcgacag ccgagttttt
     9901 ccagtagcag cacggagtct cgtgccattc ttgatttcag ggcgtcacca atacaggtga
     9961 caaagaaatt gacattcaca actaatactc cattacttca tgcccattta tgcccgatgt
    10021 ttccccccca ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc
    10081 aacataccac ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa
    10141 taactaattt tatttctttc ggcatcgctc acattttatg gggaaatcga cgttcatttt
    10201 tggcataaga aatccattta aaaaggctac atcccttata cctattcaga gctacttatt
    10261 caggggttat tgagatatac actgatggtg tacaaaaaaa tcaccgacaa taatcacgta
    10321 tcaagtaata gtcggggttt tatttaattg aagcaattta gtttgcattt ttatttaaaa
    10381 actgagctag agtccatcag aaaaaaatat ataaagccca aaccaccgtt ggaaatagaa
    10441 atagttgaaa ggctcacaac tcccggaatg cggattatct ttgccctgat ttctggcggt
    10501 tttattcagg gtgcaagctg tctgaccctt tcccgatatt ctcccggagt acaaccaaac
    10561 tcgcggacaa acgccttgtg aaaagatgat tcactggcat agcctactga ctcagcgatc
    10621 accacaacag ggagcatttc ccgggaaaac atctgggccg ctatttgtag acgcaacttt
    10681 gttaatacag ccagcggcgt ggttccggaa acatcacgga aaagctgggc aaaacttgcc
    10741 cgggacatgt gagcgatgct ggccagcgat tcgacggtcc aggcgtgtcc tggcatttcc
    10801 agcatttgct gtattaccgc accaagacgt ggatgcagaa gcaaactgag aatgtttttc
    10861 tcagtattaa cctgtgcaat ccactcacgc accgcgaggg taaagaatgt agcgcagatc
    10921 tggctacaca gtgcatccac tcccggcatc gccgatctgg attcctgttg taaaaacggg
    10981 atcgcctccc tcagccagtt atattccacg ctgtggttaa ccggtgctaa aaacagcgtt
    11041 tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca accgaagagt gccgcagaca
    11101 atacaggtcg attcattatc gacatgactc aggcgatgag ctgaattttg tggtagcagg
    11161 accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg gcatttccag cttcgccgct
    11221 ccttgcgtta acgcatgcca acgaataacc gataattccc cggcaccatg tggaagctgc
    11281 cagtcacttc ctaacacgca attcttatcg atcgttcctt gtggagcgtt aagcatcaac
    11341 aaacggctaa gggcatccat atcagactcc tgagtggttt ttgattacaa acaccaacaa
    11401 tcttagtaaa taactaatct aataccatca aatggttaat catgattttt gttttgtgat
    11461 tctgattaag aaaaagaaaa taccagacga taagacaaaa tatcaagcgt tgtgaagaaa
    11521 tggtatttgc tcttttgcgt ctatagtcat gatgtcaaat gaacgcgttt cgacaggaaa
    11581 tcatcatgaa taaatatcag gcagtgatta ttggttttgg caaggctgga aaaacattag
    11641 ccgtcacgct ggcaaaagca ggttggcgag tggctctcat cgaacaatca aatgcaatgt
    11701 atggcgggac ctgtattaat atcggctgca tcccaaccaa aacattggtt catgacgcac
    11761 agcagcacac agattttgtc cgtgccatac agcgtaaaaa tgaagtggtt aattttttac
    11821 gtaataagaa ttttcataat cttgcggata tgcccaatat cgacgtgatc gacggccagg
    11881 cggagtttat caataatcat agcctgcgtg ttcatcggcc tgaggcaaat ctggaaattc
    11941 atggcgagaa aatttttatt aataccggtg cacaaaccgt ggttccgcca attcctggaa
    12001 ttaccaccac gccaggagta tatgacagca ccggattact taatctaaaa gaattgcctg
    12061 ggcatttagg tattttgggc ggcggatata ttggcgttga gttcgcctct atgttcgcta
    12121 attttggcag caaggtaacc attttagaag cagcttcgct gtttttgcct cgggaagatc
    12181 gggatattgc tgataatatc gcgacgattt tacgcgatca gggcgtcgat attatcctca
    12241 atgcccatgt ggagcgaatc agtcaccatg aaaatcaagt gcaagtgcat agcgagcacg
    12301 cccaactggc ggtggatgca ctgttaatag cttccggtcg tcaaccggct accgcttcgt
    12361 tacatccaga aaatgccggt atcgcagtaa acgagcgcgg ggcaattgtc gttgacaagc
    12421 aattacatac caccgcagac aatatttggg cgatgggaga tgttaccggc gggctgcaat
    12481 ttacttatat ttcactggat gattaccgca ttgtgcgtga tgagttgctg ggagaaggca
    12541 gacgtagtac tgatgatcgg aaaaatgtgc cttattccgt attcatgaca ccgccactgt
    12601 ccagggttgg tatgacagaa gaacaggcca gagagagtgg tgctgatatt caggtggtga
    12661 cattgcctgt agctgcaatt ccgcgtgcca gagtgatgaa tgatactcgt ggggtattaa
    12721 aagcgattgt tgataataaa acccaacgta tattaggggc atcactgctg tgtgttgact
    12781 cccacgagat gatcaatata gtgaaaatgg tgatggatgc cgggctgcct tatagcatat
    12841 tacgcgatca gatatttact catccgtcga tgagcgaatc actcaatgat ctattttcat
    12901 tagtcaaata aactcaaaat cagacgccag aacaaatatt ctggcgtctc agagaaaaga
    12961 atcttattaa ttccctgtta ctctataact tcttaatcac ttcattgatg gtattttata
    13021 tgtttaaaaa atctgtttta tttgcaacac tattatctgg cgttatggca ttttccacca
    13081 atgcagatga taaaataatt ctgaaacata tcagcgtctc gtcagtatca gcatcaccga
    13141 cagttctgga ggataccatt gctgatatag ccagaaaata taatgcttca tcctggaaag
    13201 tcacatcgat gcgaattgat aataattcaa ccgcaacagc agtattgtat aaataaggat
    13261 gttcacaatg gaaaaatacc tgcacctgtt aagtcgggga gataaaattg gcctgacatt
    13321 gattcgtctg agtattgcaa ttgtttttat gtggattggg ttattaaagt ttgtccctta
    13381 cgaggcagac agcattacac cattcgtcgc aaacagtcca ctaatgtcgt tcttttatga
    13441 acacccggaa gactataaac agtatctgac tcacgaaggc gaatacaaac cagaagcaag
    13501 ggcatggcaa acggccaata atacctatgg tttttccaac ggtcttggcg tcgtggaggt
    13561 gattattgct ctgctggttt tggctaatcc tgtcaatcgc tggttaggtt tattgggagg
    13621 actgatggca tttaccacgc cgctggtaac actctcattt ttaatcacca ccccggaggc
    13681 atgggtaccc gcattgggtg acgctcatca tggtttccct tatttatccg gtgctggtcg
    13741 cctggtattg aaagatactc tgatgctggc aggtgctgtc atgataatgg cagattcggc
    13801 gcgggaaatt cttaaacaac gcagtaatga atccagttca acgttaaaaa ctgaatattg
    13861 ataatccgaa cactgtctgt tgttcacttt ttcaggcgtc aggccccctt tctgatgatg
    13921 aagcctgacg ccatattacc ggagatatga cagagtgaag atcaatatca ctcccctgtt
    13981 aaaagattct tgtcgtctca ggtaaaatat atgattaata tcaatcatgt atttttacag
    14041 caagacaacc acgaacaaat tcaggattaa aatgttttac cgactcccca acataaccca
    14101 aatcaagcga actaatttgt gccatttctt tctctgtcag tgagaaatcc cagatagcaa
    14161 aattttcttc gatacgctgt tgatgtgtcg atttcggaat aacgaccact ccccgttgaa
    14221 tattccaacg cagaatgact tgagaaatcg atttttgatg ggcatctgca atactctgaa
    14281 gcagattatt ttcaaagggt ttatgtcgtc caccacctaa cggagcccat gcttcaggct
    14341 gcacgttata atacttcatg gttgccagcg cctctggttg ggcaaaatag ggatgcaact
    14401 cgacctggtt gaccatcggt ttaactctta ccgtttcaca aaagttcgcc agaacatgag
    14461 gatagaaatt ggaaacccca attgctttta atttgccctc ttcataggca tcttcgagtg
    14521 cacgccacgc actgaaataa tcgcgcatag cctggtgcaa taaatagagg tcgaagtact
    14581 ctagtcccga tttttttaat gatgcttcaa tacctgctgc tgccgtatcc tgattcaaca
    14641 tatcctgcac ccacaattta gacgttataa ataactcatc tcgggtacat aaaccttcag
    14701 atatagcttc acgaacggct tcaccgacgg catcttcatt accgtatacc gctgcagtgt
    14761 cgatgagtcg ataacctgtg cggatagcgt tcaacactga ctgtttgcac acgtttttat
    14821 cggtaacctg aaaaacacca aatcccatca tcggcatttt cagattgtta cttaatacgg
    14881 aaaattccac tgtcagtccc tccattaggc atgaacaatg agtctacgtt aaaacgtaac
    14941 ctcaaattag tacgtggatt ttgatatcac ttatgcaaaa aattcattaa taatgcagga
    15001 ctgaaacctc tctattttcg gggacaacga agcagacgct accagtgctt ttgccttcgc
    15061 ccttgctgtt tttgatacac ttagggccca gggcataacg aaaatgtgcg atatgacgaa
    15121 gaatcaatgg agaatgtgat gatcaggcag aagattctac agcagctcct ggagtggatt
    15181 gagtgcaatc ttgagcaccc tatttcaatc gaagatatcg cacagaaatc tggctacagc
    15241 agacgcaaca tccagcttct gttccgaaat ttcatgcatg tgcctttggg agaatacatt
    15301 cgcaaacgaa ggctttgtcg tgccgccatt cttgtccggc tcaccgcgaa atctatgctt
    15361 gatattgcac tctctttgca ttttgattca cagcagtcat tcagccgcga atttaaaaag
    15421 ttattcggct gctctccccg tgtataccgc caccgtgatt attgggatct cgcaaatatc
    15481 ttcccttctt ttttaatacg tcaacagcaa aagaccgagt gtagattagt caactttcct
    15541 gagacaccta tttttggcaa ttcatttaaa tatgatattg aagtgtcgaa taaatcaccg
    15601 gatgaagaag tcaaactgcg acgtcatcat ttagccagat gtatgaataa ttttaagacg
    15661 gatatctatt tcgtctccac gtttgaaccg tcaacaaaat cggtcgattt gctcacggtt
    15721 gaaacttttg ccggtacggt atgcaaacaa accgattcag agatgccgaa agagtggaca
    15781 attaaccgag gactgtatgc ctctttccgc tatgaaggtg agtgggagca ctaccctgaa
    15841 tgggcgcgaa acctctatct gatggaatta cccgccaggg ggttagccag agtgaacggc
    15901 agtgatattg agcggtttta ttacaatgaa aatttcgtcg aaattgatag caataacatt
    15961 gtttgcgaaa tttttatccc cgttcgtccg gtttagtttt cgctatatct aaatgaattt
    16021 ataacgagac tactgtcagg aaattcaata aataatggcg ttgaagatgt caacgccatt
    16081 attgtttatt agaatgttac tttcatattc ttaaaattat ttcatcgcct cctcgtcctt
    16141 tgccgtatcg gcatccaccg cggtaatggt caccgtcact ggcttcattt cgtacccgcc
    16201 ataggtcagg gcgttaaatg tgaacgtata gacaccaggc ttgctggtac taaatttacg
    16261 cgtgattttt ccgctcacga aatggtcatt tttcgacggc aggaactgat aatccttttc
    16321 cgtcacccct tccggcgtct gaatgtcgac ccacatattc ccgccaacgg ggttgtttgc
    16381 cgacatcagt gtcacctcca gcttcgcagt cgatttccca tccgccacca gagattgcat
    16441 atcagatgat gcggtgagta ccgcgttttt atcatccgca acgaatttag attcggcagt
    16501 tttcgttgat acctgaccgt tggtctgact cacttgcgcc ttgagtgtgt atgtcgccgc
    16561 aacagtagtg gtagcggtga aaatagcctg cccttgctca ttcgttgtcg cagtgccatt
    16621 cggttccagg tttaacgaag ccgggctggc agtcagcgtc acttcgctat ctttcaccaa
    16681 attgttattg gcatcagtca ccgtaacttt atacgtttgt ttggctttgc cgtccgccag
    16741 cgcatagttg cccatcacct caataccgga gacggttgcg gtagccttat cggctacaaa
    16801 cgttatagtc tgcgtattcg aaggctggct attccctgcc gatgccgtga tctcatacgt
    16861 tccggcagtc actgaaacca cctgcaactc ggctttcccc tcatcgttgg ctttcaccca
    16921 gacattatcg cctgtaagcg gcttgacacc ccggggcaga ttaaaggtca ccaggctccc
    16981 tggaacaggg ttaccatttt tatcctcaac gttcgccgtc agcgtaaagg catctttgcc
    17041 gtttgccact ttttgagcag cggtgtctac ctgcaggttt gcctgaccgg tgctggcatc
    17101 cgcgttgaat ttcaccgtga ccgtcttctg agcattattc actgaggccg tgatgctgtg
    17161 ttccccggca accgttgaca tcagctcaat gtcggcttta cctgccgcat cggttgttac
    17221 cgtattcccc gtcttgctgg tcacaccttt cggcagagtc agcgtcacat tttgtccctg
    17281 caacgggtta ccataggtgt ccacaacggt cagggttacc tggttagtcg attgtccatt
    17341 agccagctgg ttatcaacct tcaccgtcat atcacgaatc tcagccttag atgcgtcacc
    17401 agcaacattc agcaccagtg gctgagcaac ggcattttgg ccgttcactc gcggcatcac
    17461 agacaactgg cccgcggcag atcccagcgt taaggtcgcc acgtagaccc cattacttgt
    17521 ttctgtccag tctcctgttg aaggtcgctc tgtccccgtg ctggcggcac cactaaactg
    17581 cggtgcatct ggcttcagcc cggtgacaag gttcccgtac gcatccttca cggtgaaggt
    17641 gagtttcgtc gtcgttttga cggtcggagc cttattgtca gcaacaagcg tagagttcgc
    17701 tgatgacatc tcccccgcaa tgacagtcaa ctgcgcggct tcggtggctg ctggctggcc
    17761 gttgaatagc ggcatgacga gaagctcacc cgtctttcca cctgttgtca gcgtagcgac
    17821 ataggaacca tcaccttttt cggtccaacc agaaacagtc gccccttcag acgcagcacc
    17881 cgtcaggctt gccgacaggg aaagaccact gatagcgttg ccatgtgcat cttttgcaat
    17941 aagcgtcacg gttgtgcttt cgcctgcttt tacgcgatct gcggctacag acactgtcga
    18001 ctggcctgaa gataacgcat cagccaccac ggttacttta gccgcattcg ctgccgcatc
    18061 ctgtccattt agcttcggca taacctctaa ttcacccgcc gtagtgccga gagaaatctg
    18121 cgcagtccag gtcccgtcgc cattatccgt ccagcctgat gccgtagaac ctacagcagc
    18181 tgcgcccgat aatgacggtg catccggatt gagaccagtt acagggttgt cattagcatc
    18241 ttttgccgtc cagattgccg taactgtacc gccaaccacc ggtttatcag gattcagtgt
    18301 gatggacgaa tgtgctgcat caagcggccc ggcaacaaac ttcagcgttt gttgtaatgt
    18361 gcccagctga tactgctcgc tgaaagcacg cacaacaacg tttccggcac gggttgaaga
    18421 aaccgtggcg ctgtaaacgc ctggttttat ttccgaaatg gcaccaacgg ttacaccatt
    18481 agtgtcttgc ggaacaaatc gcaagcggct ggcttctccc gtcaccggat taccctcgga
    18541 gtccaccgca gtcaacgtca acgtataggc ttgctgacca tcagcaacca catcacctga
    18601 cggctcgtta gcgctcaggg tggagtttgc cacatccatc atcgtggccc gcagttctgc
    18661 agtgacggtt ttgctcatgc catcaacgct aacagtaatc gttgcctcac ctgactgcgt
    18721 tccggtagta aagacagact gatacacccc tgcttcagtt tcggtgaact cacccagtgt
    18781 tggctttgcc tgtgatttag tggcctttag ggaacgagtc acaatatttc cagccggttt
    18841 gaaggctagt tcagtcttga tctgatcttt catgcccgtg actggctgcc cctcggcgtc
    18901 gcgcagagac agcaccagcg gtctttgctc attaccgtta gcaagcattt gaatacggct
    18961 ctgaccgtca agcgttaacg ccgtgcgatc ggcgctcata cctgctccgg taatgaccac
    19021 ctctgtctgc acgcgttttg aggcattgcc tttgttatcg taggcaaccg cagaaatcgc
    19081 ataataattg tctttgcctg gacgataagc cgggagcgtt acttgccact gactaccctg
    19141 accggtaatt ttgccaccct cagccagtaa tgacggcgct tcccactgca catttttcag
    19201 tccgtgagtt gctttgctga ccacaagccc cagggaaagt gtctgaccac ccttaccttc
    19261 aatacgttca ggcagagcaa tacggatcac ttcagatttg cggtactcaa gaacgatgtt
    19321 gttattacgc tcaaccaggt catagcggct gcctgccagt acccgacgct cgcgaatgct
    19381 gtctgtatcg agttgtttcg ccagaggttc gccaatccga taattaactt ccaggccaaa
    19441 gcgagtgtca ttctcaccac tcttgccctg cttatgcccg gcgctcagtg tcagaagagg
    19501 cactggcgta taggtcacct cggcagaaat agcatgcggg tctttctggc gcttatcttt
    19561 accaaacagc ccgacttcat cgccataata ctgttcatac atcaggcttg cgccaagctg
    19621 cggccaggcg ggtaaatagc cctcagcacg aatatcccag ccattcgccg ggcgttcctg
    19681 ataatcctca acatccggcg attttttcca gccagaagcc cggatataac cattggcgct
    19741 cagtttcaga taatcgcgcc agtattccgc accaacacca atgcgggtat gactacggga
    19801 taaatcatgg tcgataaagg tgttcacccc cgccatccag tcatttcctg aaaaatgacg
    19861 ccagccaaaa ccaatatttg actgagtacg atcgtcggta cgatgtattg ctccctgagt
    19921 gaacaacata tttgttggcg tatcataaat cggataaagc atttccagcg aagaatcctt
    19981 cagcgagaaa tctttatcga cattcagttt gacgcgcgca gtaccatatt tcccgagcca
    20041 ctcctgtatt tcctggttag ctttagctgt ggccattccg gtaataaagt tacgtgtcgc
    20101 atcgctatct ggctgactgc ttaaaaatgt cccggcattt gcggcaaacg acgcgacatt
    20161 tttctccacg ttattatcag cagttaccgt agtatttccc atgctcaacc gtggctgaac
    20221 cgcatgctgc gcacgtgccg ccattactgg ggtaaaggtg acagcgagtg gaaaaagaac
    20281 ctgaacagag atatttgccc acgccacgca gcgggtcaga actgaataac gaaatcgtgg
    20341 ttgtttatga cctgttttat aatgtgacat tgagataatt tcctgatgaa cgaattaacg
    20401 aacataggca gacacaaata atggcgtctg cttgtcagaa aaaccgggga tttcccgatt
    20461 tccttttccg cagaaaagga aataccttta tgtaagaaag tgcaccgcca cctaatatat
    20521 ccagtgcctg tagccactca tcaggtggtt gggcgcggga ttatattttt atttatgggg
    20581 gaaaatttag ctttttgtgc aagtcattaa atagatacct aagttatatt tcttactctg
    20641 cggacagtga ataattcact attcagagtg aataacaatg acctgccttt aattaaaact
    20701 attgaaaata cgtcgaaata ggtatatatg ccaggataaa ccaatatatt ttttatggat
    20761 attcattttc cttcatcgga ctgtaaattg cggagatatc gaacttgcac tacccatcgc
    20821 aacactcaaa cagatttatg aatttcgttc ataacccctg acaaattctg ccgtctaatc
    20881 gagcttttcc ctttacgtta tgcttctcat gaacgataac acaacttgtt catgaattaa
    20941 ccattccgga taaactatgg gatcaaattc aggcatcgaa aggaactggc tggtttccgt
    21001 taatggatta aatatctatt attctcccga gagtaaaact atcaaatcaa tgagtaaccg
    21061 atacagcctc acaaattctt tcagataatt aacccgtcac tgcggcacgt tctctcataa
    21121 ggcgtgcctg cagtgtaatc cagtatgtat ccggcatagt tgacggttta atagaactca
    21181 attcctttcc caggttacca acaacgatat cttgtataac accccaggag atacgacatt
    21241 atgatgaaat gaggtatgct cctcagcgcc aatcaacgcc cggactgggc cagtcttgag
    21301 atcagactca ttcccggaac tgacttcagt cgacatatat caatgacgtt ggtgcaataa
    21361 aagatagctg atgtaataca tctattcaaa tttgtgatac atgtcaaaaa attggttgac
    21421 caaactcgtt atttatataa gggcacttac gaagtgcact cttttttaaa gcgaggaagt
    21481 accaatgaaa gagaataaag tacaacaaat cagtcataaa ctgattaata tcgttgtttt
    21541 tgtcgcaatt gtagaatacg cctatttatt tctccatttc tattaataac ggcaataaac
    21601 tgttcacttc agtgatattt aaaatatgca tcctctccct ttttgtaagt aattattata
    21661 tccgtgggag aggaatacac gctgtcaggt aatcaatcat actgcgataa atcatcggcc
    21721 agtaaagtgg agataacctc cattctcgaa aaatccatac tctcagcaaa accatcatca
    21781 atcactcatc caggcgttta tgggagcgtc gccaacggct gctaacaatg ccagacttcc
    21841 ccgttgggga aattccacat accacaaatg ggcacaggga ttgggtgttg aaatgatccg
    21901 gatgagcatg tatctttacg gttatgttat aacataacag gtaaaaatga tgaagcccaa
    21961 tatccatcct gagtatcgta ctgtggtgtt ccacgacacc agtgttgatg agtactttaa
    22021 aatcggctcg actatcaaaa cagaccgtga gattgagctg gatggcgtaa cgtatccgta
    22081 cgtgacaatt gatgtctctt ctaaatcgca cccgttctat acagggaagc tgagaacagt
    22141 gacatcagaa ggaaatgttg cacgattcac ccaacgtttt ggtcgttttg ttagcacgaa
    22201 aaagggggcg tgatgaaagt tcttaactct ctgcgtaccg caaaagaacg ccatccagac
    22261 tgtcagattg tgaagcgaaa aggacggttg tatgtgattt gtaaatctaa tccacgtttt
    22321 aaggccgttc agggtcgtaa gaaaaaacgt tgattcaaaa ttcgacgaat taacgatatt
    22381 tgtctgatta ataatcagat cggattaatg ttggtgtgtt tataacacca acattaattt
    22441 tcctggggat atattcttcc tgttcatttg aggccaactg cctgacgttt ctctccgaat
    22501 attccattat cttaatgttg acttgttgac cagcttcgcc cctgtatgct ggcatcaacc
    22561 ctcttttaga ctgaacacgc cactcagtct cctccctttg cggcgcagcc tgcattttca
    22621 ctcaaactgt taagatgata aatgtggtaa atctgttggt actaacataa aaacgtttac
    22681 gccacaggaa cagtctgatc caccggtaac ccgtagccga cgttcgagtg ccagttagag
    22741 taacgcgcac agataactgc actcagtgcc ctgacaaaga ggccatcgtt cctgtgacag
    22801 ctggcagcct tcgtttaact tcacttaatc tggctcttgg gggcttaccg aacagatgac
    22861 gtacatacgc ccgttcaatt ttccatcact tattgtaatg aacacctata accattttgt
    22921 gcggcatgtt aatccattaa aatttcttac tgattggcaa atcatcttca atgacagctc
    22981 attatagttt tatattctat cccttaccct taaaacttgt ttttttacta gtccatcaca
    23041 cagcgcatta agactattcc taacacttca gggcaaagtt cctgaccaat ataaaatgca
    23101 agtaagaatt gaacgttata ttgccaataa ccttatgaaa ccaaatgtct ttttcttctt
    23161 atcaaaaaag caatattttc agtttttcta aatattgact taaccattga attccttttc
    23221 cgttcacata ttgacactca tcgggaaaaa aacataaatt taagcccaat cgaaaataat
    23281 taaacttaat ctcgtttaac ctttattgat atgtactacg tatcttattt acttccggtt
    23341 tactaacgaa actgaatgca cctgtaaaaa ttacaggttt ggaaagtagt gacatggcaa
    23401 agtgattaca gcagggacta tgaggttaaa aaccatatgg aatgtcaaaa ccgttctgat
    23461 aaatacatct ggtctcccca tgacgcctac ttctataaag gactatctga actgattgtg
    23521 gatatcgaca gattaattta tctatcgttg gagaaaatta gaaaagattt cgtgtttatc
    23581 aatctcaata cggattcttt atctgaattt ataaaccgtg ataatgaatg gttatccgcg
    23641 gtaaagggga aacaggtcgt attgattgcg gccagaaagt cagaagcctt agcaaattat
    23701 tggtattaca acagcaatat taggggcgtg gtatacgcag gactgagtcg tgatattaga
    23761 aaagaactgg cctatgtgat taatggcagg ttcctgagaa aagatattaa gaaagataaa
    23821 atcacggacc gggaaatgga aattatccgc atgacggccc agggaatgca acctaaatcg
    23881 attgccagaa ttgaaaattg tagtgtgaag acagtgtata cccatcggcg taatgctgag
    23941 gccaagctgt actcaaaaat atataagttg gttcagtaaa ctccaggcta gttagtttta
    24001 aaaatgactc actgggacat cacgtcctca attcaactcg ggaagaaatg caatgaaaaa
    24061 aaaggttctg gcaatagctc tggtaacggt gtttaccggt ataggtgtgg cgcaggctgc
    24121 tgacgtaaca gctcaggctg tagcgacctg gtcagcaaca gccaaaaaag acaccaccag
    24181 taagctggtt gtgacgccac tcggtagcct agcgttccag tatgccgaag gcattaaagg
    24241 ttttaactca cagaaaggtc tatttgacgt ggctatcgag ggtgactcaa cggctaccgc
    24301 ctttaaactg acctcacgtc ttatcaccaa cacattaacc cagttggata cctcaggttc
    24361 cacactgaat gtgggcgtgg attataacgg cgcggcagtc gaaaaaactg gcgataccgt
    24421 gatgatcgat accgccaacg gcgtactggg cggcaacctt agcccgctgg caaacggtta
    24481 caatgccagc aatcgtacca ccgcacagga tggtttcact ttctccatca tcagcggcac
    24541 caccaatggt accaccgcag taaccgatta cagcactcta ccggaaggca tctggagcgg
    24601 cgacgttagc gtacagttcg acgcgacctg gaccagttaa tctctctgat gtaccagcag
    24661 gggtagcccc cctgctttat tcctggacgg aattgttatg aaaaagcacc ttctgcctct
    24721 cgctctgctg ttttccggaa tatctccggc ccaggcgctg gatgtcggcg atatatcatc
    24781 gtttatgaac agtgacagca gcacgctgag caaaacgatc aaaaacagta ccgacagtgg
    24841 tcgccttatc aatatccgtc tcgaacggct ctcttcaccg cttgacgacg ggcaggttat
    24901 ctcaatggac aagccggatg agttgctact cactcccgcc agcttgctgc tacccgccca
    24961 agccagcgaa gtgatccgct tcttctataa gggacccgca gatgaaaaag agcgctacta
    25021 ccgcattgtc tggtttgatc aggccctcag tgatgcgcag cgcgataatg ccaaccgcag
    25081 cgctgtggcc actgcttccg cccgcatcgg caccattctg gtcgtcgccc ctcgtcaggc
    25141 gaactaccac tttcagtacg ccaacggctc cctgacaaat acaggaaatg cgacgctgcg
    25201 gatcctcgct tacggcccct gcctgaaagc cgccaatggt aaggagtgta aagagaatta
    25261 ctacctgatg ccaggcaagt cgcgtcgttt tacccgcgtg gacacggcgg ataacaaagg
    25321 acgggttgca ctttggcagg gtgataagtt cattcccgtg aaatagatag ctgtgcagat
    25381 ggataacgac aatgccttta cgacggttct ccccaggact gaaagcccag tttgccttcg
    25441 gcatggtctt tttgttcgtt caacccgatg ccagcgctgc tgacataagt gcgcagcaaa
    25501 taggtggggt gattattccg caggccttca gtcaggcgct tcaggacggc atgagcgtcc
    25561 cgctctatat tcatctcgcc ggtagccagg gtcgccagga cgatcagcga atcggcagcg
    25621 cttttatctg gctggatgat ggacagctac gcatccggaa aatacagctg gaagagagtg
    25681 aagataacgc cagtgtcagc gaacaaactc gacagcagct gatggctctg gcgaacgccc
    25741 cgttcaatga ggcccttacc atccccctga ctgacaacgc gcagctggat ctcagcttgc
    25801 gccaactgct gctgcagctg gtggtcaagc gcgaagcgct gggcaccgta ctacgctcac
    25861 gtagcgaaga catcgggcag tccagtgtta acaccctcag cagtaatctg agctataact
    25921 tgggcgtcta taacaaccag ttgcgtaacg gcgggagcaa cacatccagc tatctgtcgc
    25981 tgaataacgt tactgcgctg cgcgaacatc atgtggtgct cgacggctcg ctgtacggga
    26041 tcggtagcgg tcaacaggac agtgaattat ataaagcgat gtatgaacgc gattttgccg
    26101 gtcaccgatt tgccggtgga atgctcgaca cctggaactt gcagtcctta gggccgatga
    26161 ccgccatttc agcagggaag atttacggcc tttcctgggg aaaccaggcc agctccacca
    26221 tcttcgacag cagccagtca gccacgccag tgatcgcctt tttaccggcg gcgggcgaag
    26281 tacatctcac ccgtgatggg cggctactaa gcgttcagaa cttcactatg ggcaatcatg
    26341 aagtggatac ccggggtcta ccgtacggga tttacgatgt ggaagttgag gtgatcgtta
    26401 acggtcgcgt gatcagcaaa cgcccccagc gggtcaataa gctgtttagc cgggggcgcg
    26461 gcgtcggtgc accactggcg tggcaggtat ggggcggtag ctttcatatg gatcgctggt
    26521 cggaaaacgg gaaaaagacg cgaccagcta aagagagttg gctagcaggt gcctcgacct
    26581 ccggctcatt gagtacgctt agctgggcgg caacgggata tggatacgat aatcaggcgg
    26641 tgggtgaaac ccgtctgacg ctgccgcttg ggggggcgat caacgttaac ctgcaaaata
    26701 tgctggccag tgacagctca tggagcagca tcggcagcat cagcgccact ctaccgggag
    26761 gctttagttc gctgtgggtt aaccaggaaa aaacccgcat tggcaatcaa ttgcgacgta
    26821 gcgatgccga caaccgtgca atcggcggca cactcaacct gaactcactg tggtcgaagc
    26881 tgggcacatt cagcatcagc tacaatgatg accgccgtta caacagccat tattacacgg
    26941 cagattacta tcaaaatgtc tacagcggta cctttggttc gcttggcctg cgggccggta
    27001 ttcagcgcta taacaacggc gacagcaacg ccaatacagg gaaatatatc gctctcgatc
    27061 tctcgctacc actgggcaac tggtttagcg cagggatgac tcatcaaaac ggctacacca
    27121 tggcaaacct gtcagcacgc aaacagtttg atgaaggaac cattcgcact gttggtgcca
    27181 atctgtcacg tgccatctcc ggcgataccg gtgatgacaa aaccctcagc ggtggggcgt
    27241 atgcacagtt cgacgctcgt tacgccagcg gaacgctgaa cgtcaatagc gcggcggacg
    27301 gctacatcaa taccaacttg accgccaatg gcagcgtcgg ctggcagggt aaaaacatcg
    27361 ctgccagcgg gcggactgat ggcaacgctg gggtgatatt caacaccggg ctggaggacg
    27421 acggtcagat cagcgccaaa atcaacgggc ggattttccc gcttaacggc aagcgtaact
    27481 atctcccgct ctctccctat ggaagatatg aggtggagtt acagaacagc aaaaactcac
    27541 tcgacagcta cgatatcgtc agcggccgca aaagccatct gactctctat ccaggcaatg
    27601 tcgctgtcat tgagccagag gtgaagcaga tggttaccgt ctccggtcgt atccgtgcgg
    27661 aagacggcac actgctggct aacgcacgga ttaacaacca tatcggccga acccgaaccg
    27721 atgaaaacgg cgagtttgtc atggacgtgg ataagaaata tcccactatc gattttcgct
    27781 acagtggcaa taaaacctgc gaagtggcac tggaactcaa ccaggcgcgc ggtgccgtct
    27841 gggtcggtga tgtggtctgc agcggcctct catcgtgggc ggaggtgacg cagacaggag
    27901 aagagaatga gagttaacct actaataacg atgataattt tcgcgctaat ctggccagta
    27961 actgagctca gagcggcagt gagcaaaaca acctgggcgg atgcaccggc acgcgagttt
    28021 gtgtttgtcg aaaacaactc agacgacaac tttttcgtca ctcctggcgg ggcgctggat
    28081 ccgcgcctga ccggtgccaa ccgctggacc ggtttaaaat acactggttc aggaaccatc
    28141 tatcagcaaa gcctcggcta cattgataac ggttacaaca ccggccttta taccaactgg
    28201 aagtttgata tgtggctgga aaattcacca gtttcatctc ctttaactgg cttgcgctgc
    28261 atcaactggt acgctgggtg taatatgacc accagtctta tcctgccgca aaccaccgac
    28321 gccagtggat tttatggcgc gacggtgacc agcggcggcg cgaagtggat gcacggcatg
    28381 ttgtcagacg cgttttacca gtatctgcaa caaatgcccg tcggcagcag ctttacaatg
    28441 accatcaatg cctgccagac ctctgtgaac tatgacgcca gcagcggcgc acgctgtaag
    28501 gatcaggcct ccggcaactg gtatgttcgc aacgtcaccc atacgaaagc agcaaatctg
    28561 cggttaataa atacccactc gctggcggag gtatttatca acagcgacgg agtaccgact
    28621 ctgggcgaag ggaacgccga ctgccggaca cagaccatcg gcagcctttc aggattaagt
    28681 tgtaagatgg tcaactatac cctgcaaaca aacggactca gcaacacctc aatccatata
    28741 ttcccggcga tcgccaactc gtcgttagcc tcggccgtcg gggcgtacga tatgcagttc
    28801 agtctgaatg gcagttcatg gaaaccggtg agcaataccg cctattacta caccttcaac
    28861 gagatgaaga gcgcagactc gatctatgtt ttcttctcga gcaacttctt taagcagatg
    28921 gtgaacctcg ggatcagcga tatcaacacc aaagatctat tcaactttcg ctttcagaac
    28981 accacatcac cggagtctgg ctggtatgaa ttttctacct ccaacacgct gattatcaaa
    29041 ccccgtgatt tcagcatcag tattatctcc gatgaatata ctcagacacc gtcgcgggag
    29101 ggatatgttg gcagcggcga gtcggcactc gatttcggct atatcgtaac caccagcggt
    29161 aaaacagctg ccgacgaagt gctgatcaag gtgaccggac ccgcgcaggt gattggcggg
    29221 cgctcctatt gtgtcttcag ctccgatgac ggtaaggcga aagtaccgtt cccggcgaca
    29281 ctttccttta ttacccgcaa cggagctaca aaaacctacg atgccgggtg cgatgatagc
    29341 tggcgggata tgaccgatgc gctgtggttg accacaccgt ggactgatat ctctggcgaa
    29401 gtggggcaga tggataagac cacagtcaaa ttttcgattc caatggataa cgccatttcg
    29461 ctgcgtacgg tagatgataa cggctggttt ggcgaagtca gcgcttcagg agagattcat
    29521 gttcaggcga cgtggcgtaa cattaactaa ggccctgctg acagcggtct gtatgctggc
    29581 ggcacctttg acacaggcga tttcggtcgg caatctgaca ttttcgctgc cgtccgagac
    29641 tgactttgtc agcaaacgtg tagtgaataa caacaaaagc gcacggatat accgtattgc
    29701 catcagtgct attgatagcc cgggcagcag tgaattgcgc acccgaccgg tggatggtga
    29761 actgcttttt gccccccgcc aactggcgtt gcaggctggt gagagcgagt attttaaatt
    29821 ttactatcat ggtccacggg ataaccgcga gcgctactac cgggtctcat ttcgcgaagt
    29881 ccccactcgt aaccagacaa gacgtagccc aaccggcggc gaggtcagca cggagccggt
    29941 ggtggtgatg gataccattc tggtagtacg accacgtcag gttcagttta aatggtcctt
    30001 cgacaaggtg acaggaacgg taagtaacac tggcaacaca tggtttaagc tactgattaa
    30061 gccaggatgt gattcgaccg aagaggaagg cgatgcctgg tatctacgtc cgggagacgt
    30121 ggttcatcag cctgagttac gtcagccggg gaatcattat ctggtctata acgacaaatt
    30181 cattaagatt agcgattcct gtccggctaa gcccccttcg gcggactaag acttatccat
    30241 cggtcgaggg aagaattcca cctcagagct ccaaaaatcg tttaaatgat gtggaagcga
    30301 tcgtcaatgt cacctgtaac gatgggcttt ctcatatgca caccatagtg ttcaggagac
    30361 acggaaaggt atcaggcagc ctgctaatga gtggtttaat cacgaattag tacgtaaaat
    30421 cggtaacggc tggaaatcat tcaatactca cactatcgaa agttccccag ccaaccgcgg
    30481 tacgttctta catacgatgt accgctgttc tctttacgat ttttagctgt actggtgaat
    30541 tatgagcaat ctgaatccat gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc
    30601 tttttactgg gccgaagccg acgatgctgg cggtactatt cccgccaggc tcactgaaca
    30661 aatatcccct tttcaccgat gcatgagcgg taccaatcag aaaaaccccc gatgtattgc
    30721 ccttgcagga accccgggca aaaatgcctg ctgcacgata tataaaaatc gatcgtccac
    30781 atgcagagaa tttgccatgt ctggtgagaa cggagtcgtc aatgaggctt gcaatcgtgc
    30841 aagggctaaa tacgggc
//
LOCUS       DAZFKM010000050        30784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFKM010000050 DAZFKM010000000
VERSION     DAZFKM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHKAY_002335"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHKAY_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO5701003.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHKAY_002336"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHKAY_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO5701004.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKAY_002337"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKAY_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO5701005.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKAY_002338"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKAY_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO5701006.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESMDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKAY_002339"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKAY_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO5701007.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKAY_002340"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKAY_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO5701008.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKAY_002341"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKAY_002341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO5701009.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKAY_002342"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKAY_002342"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO5701010.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHKAY_002343"
     CDS             12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHKAY_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO5701011.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHKAY_002344"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHKAY_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO5701012.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHKAY_002345"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHKAY_002345"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO5701013.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHKAY_002346"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHKAY_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO5701014.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHKAY_002347"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHKAY_002347"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO5701015.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHKAY_002348"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHKAY_002348"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO5701016.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHKAY_002349"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHKAY_002349"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO5701017.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHKAY_002350"
     CDS             complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHKAY_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO5701018.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19793..20566
                     /gene="nudC"
                     /locus_tag="ACHKAY_002351"
     CDS             19793..20566
                     /gene="nudC"
                     /locus_tag="ACHKAY_002351"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO5701019.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20606..21670
                     /gene="hemE"
                     /locus_tag="ACHKAY_002352"
     CDS             20606..21670
                     /gene="hemE"
                     /locus_tag="ACHKAY_002352"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO5701020.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21680..22351
                     /gene="nfi"
                     /locus_tag="ACHKAY_002353"
     CDS             21680..22351
                     /gene="nfi"
                     /locus_tag="ACHKAY_002353"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO5701021.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHKAY_002354"
     CDS             22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHKAY_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO5701022.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23171..23443
                     /gene="hupA"
                     /locus_tag="ACHKAY_002355"
     CDS             23171..23443
                     /gene="hupA"
                     /locus_tag="ACHKAY_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO5701023.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHKAY_002356"
     CDS             23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHKAY_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO5701024.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHKAY_002357"
     CDS             complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHKAY_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO5701025.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24816..26192
                     /gene="zraS"
                     /locus_tag="ACHKAY_002358"
     CDS             24816..26192
                     /gene="zraS"
                     /locus_tag="ACHKAY_002358"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO5701026.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26189..27514
                     /gene="zraR"
                     /locus_tag="ACHKAY_002359"
     CDS             26189..27514
                     /gene="zraR"
                     /locus_tag="ACHKAY_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO5701027.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHKAY_002360"
     CDS             complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHKAY_002360"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO5701028.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHKAY_002361"
     CDS             complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHKAY_002361"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO5701029.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7258 a         7983 c         8421 g         7122 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag catggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aagtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattacg cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgattc cgggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgttactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gtttttttcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga cggcaattgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggcac
    15001 tgtggcctgc tggataataa tctccagcat ggcgcgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttaaccag tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt acagcgcagc aggtgtaccc agcccgccca gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcaaaatt tcggtccaca catggagcaa aaatgggcga ctttgccgga ctcttggggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc agagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac ggatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cgcggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcagggg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agccccgcaa atggggcatt gaatgtaaat
    19201 tacgcgttaa cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag
    19261 cagaatcagc ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa
    19321 aacctgttga aactcaaggt aattatcatg atcgatggcg gtttccagac tggaatcgta
    19381 gtaatccata atctgttgag tattggcttc gagttgcggc caaatcttcg cggcgcgcgc
    19441 gagttgcccg ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc
    19501 agacaagtaa tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat
    19561 gtacgattct ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt
    19621 acgtacatgt agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt
    19681 atcgagctgg ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga
    19741 aatgtaaaaa ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg
    19801 tataattgaa aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt
    19861 gccgaaggga gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc
    19921 actacaaatc ggcgaatggc agggggaacc tgtttggtta gtacaacagc agcggcgtca
    19981 cgatatgggg tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg
    20041 acgaggcgta caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca
    20101 tgaaatgtat ccgagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta
    20161 ctacccgcaa atcgccccct gcattattgt tgctatccgt cgcgatgatt cgatcctcct
    20221 cgcccagcat acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt
    20281 gggcgaaacc ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt
    20341 taaaaacttg cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc
    20401 gtttatggcg gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc
    20461 gaactggtat cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg
    20521 tctgatagaa gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg
    20581 cctgacgcac taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc
    20641 gctgctgcgc cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta
    20701 tctaccggaa tataaagcca cgcgcgccca ggcaggcgat tttatgtcgc tgtgcaaaaa
    20761 cgccgagctg gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc
    20821 gatcctcttt tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga
    20881 agccggagaa ggtccgcgtt ttacctcgcc agtcacctgc aaagctgacg tcgataaact
    20941 gccaattccg gacccggaag atgaactggg ttacgtgatg aacgcggtgc gtaccattcg
    21001 tcgcgaactg aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc
    21061 gacctacatg gtggaaggcg gcagcagcaa agccttcacc gtgatcaaaa aaatgatgta
    21121 tgccgatccg caggcgctgc acgctctgct cgataaactg gcgaaaagcg tcactttgta
    21181 tctgaatgcg cagattaaag ccggtgctca ggctgtgatg attttcgaca cctggggcgg
    21241 cgtgcttacc gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga
    21301 tggtttactg cgtgaaaacg acggtcgccg cgtgccagtc acgctgttta ccaaaggcgg
    21361 cggacagtgg ctggaagcga tggcagaaac cggttgcgat gcgctgggcc tcgactggac
    21421 aacggatatc gccgatgcgc gccgccgcgt gggcaataaa gtcgcgttgc agggcaatat
    21481 ggatccgtcg atgctgtacg cgccgccagc ccgcattgaa gaagaagtag cgactatact
    21541 tgcaggtttc ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga
    21601 tgtgccgcca gaacatgctg gcgtgttcgt ggaggcagtg catcgactgt ctgaacagta
    21661 tcaccgctaa ggagtgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21721 ttcttctgtg atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21781 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21841 tccctcgctt gagctggtgg agtataaagt ggcccgcatc gccaccacca tgccttacat
    21901 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21961 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcccc gccgtcttgg
    22021 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    22081 gctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cactgatgga
    22141 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    22201 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    22261 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    22321 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    22381 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    22441 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    22501 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgcattctcg atctcatctg
    22561 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    22621 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    22681 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22741 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22801 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22861 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22921 aggccttagg gcagacctgc gtgaggcggg tgatagcaat attggtataa tttttcagca
    22981 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    23041 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    23101 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    23161 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    23221 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    23281 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    23341 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    23401 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    23461 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    23521 ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc cctttaccgc
    23581 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    23641 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    23701 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23761 gaacattcgc gcgcgttttg acgatcgcgg tgatctgagc tttatgcaac gtgaatccga
    23821 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgctca
    23881 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23941 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    24001 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    24061 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    24121 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    24181 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    24241 gccatcgcga tatcccgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    24301 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    24361 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    24421 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    24481 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    24541 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    24601 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    24661 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24721 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24781 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24841 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24901 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24961 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    25021 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    25081 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    25141 gcatggtggg aaaacagctt tattccccgc aggaaatgca gcagttacat ccgggagatg
    25201 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    25261 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    25321 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    25381 gtgagcagag aaacaccctg attatcctct tcgccctggc gacggtcttg ctggcaagcg
    25441 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    25501 tgaagcgcaa agagaagcta gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25561 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    25621 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    25681 gcgtggtaag cgagctgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25741 atctcaacac gctgattaac cactcattac agctggtagg ccaggatgca aacagccggg
    25801 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25861 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25921 gcgtgattag cgtgacggtc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25981 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    26041 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    26101 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    26161 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    26221 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    26281 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggagc gggtttttga
    26341 tcttgtgctt tgcgatgtgc gaatggcaga gatggacggc attgccacgc taaaagagat
    26401 caaagcgtta aatccggcga ttccggtact gattatgacc gcatactcca gcgtcgagac
    26461 ggcggtagag gcactgaaaa ccggggcgct ggattatctc atcaagccgc tggatttcga
    26521 caccctgcag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26581 gcctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    26641 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    26701 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26761 accgctggta acgctcaact gtgtggcact caacgaatcc ttgctggaat ctgaattgtt
    26821 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26881 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26941 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    27001 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    27061 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    27121 gctgcggcaa cggcgggagg atatccctct gctggctagc cattttcttc agcgttttgc
    27181 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    27241 ctacgactgg ccgggaaaca ttcgtgaact ggaaaacgcg gtggaaaggt cggtggtgct
    27301 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    27361 cccgctgggg caaagtcagg atattcagcc gctggtggaa gtcgaaaaag aagtgattct
    27421 ggcggcactg gagaaaacgt gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    27481 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tagcgcgcca
    27541 gccgatatct ttccggcaga agcagtcgtc ccagtgaata tcggtcatta aggcataagc
    27601 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27661 gctggtcact acctgctcgt tatccgccag tttcgtgccc gcgtggaaca ctttgccgcc
    27721 tgccacttct tccagcggca ggccgtggat cacgtcaccg gtgcggtaat cacccggata
    27781 tccacccgca gccatcacca cgccgagaga agcgcgttca tcccactctg acgttttctc
    27841 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27901 cataatcggc tgggtttctg gatcgccaaa gcggcagtta aattcgataa ccttcggatt
    27961 gccctgtttg tcgatcatca ggcccgcgta gagaaaaccg gtgtaggtgt tgccttccgc
    28021 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    28081 ggttactacc ggcgcggggg agtaagcgcc catcccgccc gtgtttggtc cggtatcttt
    28141 atcgcctacg cgtttgtgat cctggctggt ggccatcggc agcacatgct cgccgtctac
    28201 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    28261 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    28321 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    28381 cgcgcctttc tcacgcagat acgccagcgc aggttctacc tcggtgaagt tctggtattc
    28441 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28501 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28561 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28621 tgcgaaatcc aacagcgccg ggatatcggt cacgccaata gcaacgtttt gcagcgcggg
    28681 ttccagtgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28741 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaataacta atactttcat
    28801 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28861 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28921 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28981 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    29041 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    29101 tagacgatag cattggattt cacgaacttc gccactttcc agcagaacag cgcatcgcgc
    29161 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    29221 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    29281 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    29341 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    29401 atggcctgtg cggtttccgc atctagctcg cggttaaagg caatgatgcc gccgaatgcg
    29461 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    29521 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29581 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29641 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29701 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29761 cgtgggaagc gaccagcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29821 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29881 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29941 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    30001 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcaaccttca
    30061 cgggccacgg tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    30121 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    30181 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    30241 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    30301 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    30361 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctggatttg
    30421 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30481 ggcatcctta tttttcgccc acattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30541 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30601 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30661 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30721 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30781 aaga
//
LOCUS       DAZFKM010000051        30552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFKM010000051 DAZFKM010000000
VERSION     DAZFKM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(349..1137)
                     /gene="phoH"
                     /locus_tag="ACHKAY_002362"
     CDS             complement(349..1137)
                     /gene="phoH"
                     /locus_tag="ACHKAY_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO5701030.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1756..3027)
                     /gene="efeB"
                     /locus_tag="ACHKAY_002363"
     CDS             complement(1756..3027)
                     /gene="efeB"
                     /locus_tag="ACHKAY_002363"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO5701031.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(3033..4160)
                     /gene="efeO"
                     /locus_tag="ACHKAY_002364"
     CDS             complement(3033..4160)
                     /gene="efeO"
                     /locus_tag="ACHKAY_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO5701032.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4218..5048)
                     /gene="efeU"
                     /locus_tag="ACHKAY_002365"
     CDS             complement(4218..5048)
                     /gene="efeU"
                     /locus_tag="ACHKAY_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO5701033.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5714..7222)
                     /gene="putP"
                     /locus_tag="ACHKAY_002366"
     CDS             complement(5714..7222)
                     /gene="putP"
                     /locus_tag="ACHKAY_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO5701034.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7381..7590)
                     /locus_tag="ACHKAY_002367"
     CDS             complement(7381..7590)
                     /locus_tag="ACHKAY_002367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701035.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7645..11607
                     /gene="putA"
                     /locus_tag="ACHKAY_002368"
     CDS             7645..11607
                     /gene="putA"
                     /locus_tag="ACHKAY_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5701036.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11647..12285)
                     /gene="rutR"
                     /locus_tag="ACHKAY_002369"
     CDS             complement(11647..12285)
                     /gene="rutR"
                     /locus_tag="ACHKAY_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO5701037.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            12573..13664
                     /gene="rutA"
                     /locus_tag="ACHKAY_002370"
     CDS             12573..13664
                     /gene="rutA"
                     /locus_tag="ACHKAY_002370"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO5701038.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            13664..14356
                     /gene="rutB"
                     /locus_tag="ACHKAY_002371"
     CDS             13664..14356
                     /gene="rutB"
                     /locus_tag="ACHKAY_002371"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO5701039.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            14368..14754
                     /gene="rutC"
                     /locus_tag="ACHKAY_002372"
     CDS             14368..14754
                     /gene="rutC"
                     /locus_tag="ACHKAY_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO5701040.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14762..15562
                     /gene="rutD"
                     /locus_tag="ACHKAY_002373"
     CDS             14762..15562
                     /gene="rutD"
                     /locus_tag="ACHKAY_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO5701041.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            15572..16162
                     /gene="rutE"
                     /locus_tag="ACHKAY_002374"
     CDS             15572..16162
                     /gene="rutE"
                     /locus_tag="ACHKAY_002374"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO5701042.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            16173..16667
                     /gene="rutF"
                     /locus_tag="ACHKAY_002375"
     CDS             16173..16667
                     /gene="rutF"
                     /locus_tag="ACHKAY_002375"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO5701043.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            16688..18016
                     /gene="rutG"
                     /locus_tag="ACHKAY_002376"
     CDS             16688..18016
                     /gene="rutG"
                     /locus_tag="ACHKAY_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO5701044.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(18099..18272)
                     /gene="ymdF"
                     /locus_tag="ACHKAY_002377"
     CDS             complement(18099..18272)
                     /gene="ymdF"
                     /locus_tag="ACHKAY_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5701045.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18645..19241
                     /gene="wrbA"
                     /locus_tag="ACHKAY_002378"
     CDS             18645..19241
                     /gene="wrbA"
                     /locus_tag="ACHKAY_002378"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO5701046.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            19262..19489
                     /gene="yccJ"
                     /locus_tag="ACHKAY_002379"
     CDS             19262..19489
                     /gene="yccJ"
                     /locus_tag="ACHKAY_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO5701047.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19527..20768)
                     /gene="agp"
                     /locus_tag="ACHKAY_002380"
     CDS             complement(19527..20768)
                     /gene="agp"
                     /locus_tag="ACHKAY_002380"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO5701048.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(21060..22319)
                     /locus_tag="ACHKAY_002381"
     CDS             complement(21060..22319)
                     /locus_tag="ACHKAY_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFO5701049.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNGNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            22579..23499
                     /gene="cbpA"
                     /locus_tag="ACHKAY_002382"
     CDS             22579..23499
                     /gene="cbpA"
                     /locus_tag="ACHKAY_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO5701050.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            23499..23804
                     /gene="cbpM"
                     /locus_tag="ACHKAY_002383"
     CDS             23499..23804
                     /gene="cbpM"
                     /locus_tag="ACHKAY_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO5701051.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(23897..24496)
                     /gene="torD"
                     /locus_tag="ACHKAY_002384"
     CDS             complement(23897..24496)
                     /gene="torD"
                     /locus_tag="ACHKAY_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO5701052.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(24493..27039)
                     /gene="torA"
                     /locus_tag="ACHKAY_002385"
     CDS             complement(24493..27039)
                     /gene="torA"
                     /locus_tag="ACHKAY_002385"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO5701053.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(27039..28211)
                     /gene="torC"
                     /locus_tag="ACHKAY_002386"
     CDS             complement(27039..28211)
                     /gene="torC"
                     /locus_tag="ACHKAY_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO5701054.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28351..29043
                     /gene="torR"
                     /locus_tag="ACHKAY_002387"
     CDS             28351..29043
                     /gene="torR"
                     /locus_tag="ACHKAY_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO5701055.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(29016..30044)
                     /gene="torT"
                     /locus_tag="ACHKAY_002388"
     CDS             complement(29016..30044)
                     /gene="torT"
                     /locus_tag="ACHKAY_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO5701056.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
BASE COUNT         7334 a         8165 c         7674 g         7379 t
ORIGIN      
        1 ataaatcaga ggaatatatt cggtgttaaa cagtcagtca aagtatagcc cattgttgat
       61 taaacctcat aagcaaaatt ggcaccagat ctcctgtaca ggttgaatgg ccgaaaaaag
      121 ataggcagat gctcattttt ttggcttctg gaagaaaaac tctccgcgct ggcagtatta
      181 cactggtctg atgctatgac gaaaaatgcc tggtaagagt atctaaacca gactccagtt
      241 cagcttatca acacgctcaa agtcgccccc ctgaacgcta acctgaagtg aaaatctgtt
      301 gaaataaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtaggc
      361 atgcagcgta cgttggcaaa gtgccgaacg tacgcagtcc tctttaccga accggacgat
      421 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
      481 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
      541 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
      601 gactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcaattt ccaccttccc
      661 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcacgtc
      721 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tctccaggta agaagccaag
      781 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
      841 cagggcttct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
      901 ggcgaatatc agctgcttac tctcaatagc cttcagatag tgcaattgcg cttcatttcg
      961 cgcgaggatg ggcgaagtat cgcgactgtc gcgggccata ccaatggctt ctacgccgcc
     1021 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
     1081 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
     1141 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
     1201 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
     1261 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagagaggaa gtgagaaaaa
     1321 tacagagtga cgaagttgct accggaggag aaatctccgc aagtggtgga gcgtagctta
     1381 acaagacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     1441 ctgccgggaa cttccgctgc tacttaataa ttacaacaga tctcgcattc attgcaacaa
     1501 tatattcact tatatttaac cataaaacac catttcagtg acattagttt ctactggaaa
     1561 gatgacagag tgatgacaga tatgaaaaaa gctgtgtgct ttcagcagga ttcgcatcag
     1621 gcatctgcgc acgaacgccg gatgcggcgt gaacgcctta tccgcctacc gaacccgcac
     1681 cgattgtagg cataagaaga cgcgcaagca tcgcatcagg catctgcgca agaacgcctt
     1741 atccgtctat aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt
     1801 cactcccggc agcgcaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc
     1861 ttcgccattg agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtaaca
     1921 gacaaacagt agccccatat ccagttgccc ggagttggtg acgcccagtg aataactgta
     1981 gccacgacgc agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat
     2041 atggctgtcc agcgcaatca ccttcccttc cggatcgctg gcgtaatcgg gaacatcatg
     2101 ctcatgctgc attcccagcg gcgcaccggt ttgcttatca cgaccaaaaa tcgtctgctg
     2161 ttctttcagc ggagttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg
     2221 atagctgcca ccgattgtcc acgctggctc ctgctgatct gccgttaccc acaccacttt
     2281 ttgcatcaac ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa
     2341 attaatcggc gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg
     2401 cttccagcgc acgctgagca aatccggcgt atgtttgatg atatcgcgca gtgcatggat
     2461 caccgtgtcc tgggtgttgg cgcaaatttg tagcaacaca tcaccatgac ataacgccgc
     2521 atccagcgag tcgttgggaa aacgcgtcat cttctgcaac tttttcggca tctgtggcgc
     2581 aaggccaaag cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc
     2641 gggcgcaatg tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt
     2701 ttctggtgcc gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa
     2761 ccgttcaaga tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc
     2821 ctgttgtggc gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg
     2881 tgcatccggt gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca
     2941 acttcccgcc agcgccagcg caccaatgcc tttcagtaaa cgtcggcgtg acggttcatt
     3001 cacgccgttt ttatcttcat actgcataat gcttaatcca atcccagcac accgcgaagt
     3061 tgcgccagat cttccgccag cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg
     3121 gtcaatttgt cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg
     3181 acctttttaa agttggcatc gacttttgcc aacagttccg ggttggcttt ttgcagttgt
     3241 ggacgcagca gatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga
     3301 tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc tggctgccac ttcctcaatc
     3361 agtccggctg caccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt
     3421 tgcaaatcga ccacatcggt ataaagctgg tcagcgtact gatccatgcc tttggtggtg
     3481 ttgtcgccaa acaatgcttt ttccagacgg tggaaaccgg tgaattttgg atcagcggct
     3541 ttttgctcgt aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca
     3601 gcaatcggtt caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt
     3661 tcgatatcgc ctgctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg
     3721 gtttccgcca tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca
     3781 tcactttgcg ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta
     3841 gtcagcagac cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg
     3901 ctaaaaccag gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc
     3961 cactccagcg ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc
     4021 gtaatggtca tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cgtcacatca
     4081 gcggcgttag ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta
     4141 cggcggaagt taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag
     4201 agtttgcgtc gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcaga
     4261 gcaaatgcca ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc
     4321 ggcgcttcct gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag
     4381 agcaccgcac tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga
     4441 aatgcgcgaa tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc
     4501 catttaaaaa atgcaccaag attgaggcga ataccgcccc agtagagcaa gaagcctagc
     4561 accacggcag tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt
     4621 tgaaatgccg ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag
     4681 accatcatca ccagcgccca gccatgatga tttccacgct gcaatgcgct atcgactgcc
     4741 tgctccagtt gtactttgac gttgcgcgac actttgcgca tccagaaaac catccaggta
     4801 aggatcacca cggcgatcac cgccacgata ccttcaaaca gttcctgctc tttttgcgga
     4861 aattcgccgg tggtttcgtt aatgaagatc cccaagccca ggcacaacgc agcggcaagc
     4921 aacacgccaa tccacatcac accaatccat cggcctcgct gggtacgctt aagatagctg
     4981 gcaatcaaac tgacaatcag cgcggcttca agtccttcgc gcaacataat gagaaacgga
     5041 acaaacatgc cacccatcct taaacgtttt tctgagtcaa tctatgcaaa gaaaagtaaa
     5101 tcgcagtgat agcgattatc attacggtaa agaaaaatac aagcggaatg tttataaaat
     5161 gatgttgaaa tgtaaacaaa gagaggtaat gccaggggat ataaacgatt attccaggta
     5221 caaagatggg attgtttaca acgagccatg tgttcttgcc ggatgccgcg taaacgccgt
     5281 atccagccga caaaaccgtc ctgactcaat acatcaaaac ttccatgtaa aactgataag
     5341 cgggtgtatc aagcaatttc gtccatcctc gattatcaaa tctaactttt tgcccggaac
     5401 ataagaacag cgcgggtgtt ggtggcgtca aaatcgctga aacgttcccc gaaggccggg
     5461 gcagcccaca tggatgtggg ctgagggcgc gtcttacagg acgttacctc gcgcccgacc
     5521 cggtagccga aggggataag ctgaaggtac cacgcagcgg cgattttgtt cgccagagcc
     5581 cgggggtgca gggggccgcg gcgattggcc gccccctgcg cgctccctgc accattcaat
     5641 ttatatggcg aggcacatta agggagcgca acccgctgct gaagccaaac aaaaccgccg
     5701 caggctaagt cccttagctt tcctgcaacc gtgacggcgg agccgaatga tagtgcgcat
     5761 cggcctcggc aaagcgtttt tgcatcgccg ctgacggcgc tttacccagc aaactaaaca
     5821 ctacaatccc gatactgccg aagatgaagc ccggaataat ttcgtagagt cccagccagc
     5881 cgaactgctt ccagacgata accgtcagcg caccgatgat catccccgcc agcgcaccgt
     5941 tgcgcgtcat gcgtgaccac atcaccgaga acagcaccac cggaccaaac gccgcgccaa
     6001 agcctgccca cgcgtagctc actaagccca gcacgcggtt ttccgggttt gccgccagcg
     6061 caatcgccac cagcgccacc accagcacca tcacacgtcc tacccacacc agctctttct
     6121 ggctggcctg tttacgcaga aacgctttgt ataaatcttc ggtaattgca ctggaacata
     6181 ccagcagctg gcaacttaag gttgacatta ccgccgccag aatcgccgac agcagaatcc
     6241 cggcaatcca cgggttaaac agaatttgcg ccagttcgat aaacacgcgc tcggcgttct
     6301 ggtttaccgc gcctgctacc gccgggtgct cattaaagta agcaatccca aagaagccaa
     6361 cagccaccgc ccctgccagg cagaggatca tccaggtcat actgatacga cgcgcatgga
     6421 caatgctgtg gtgagagtct gccgccataa aacgcgccag gatgtgcggc tgcccgaagt
     6481 aacccagccc ccaacccatc agtgagataa tggcgacaaa gttcagccct ttgagcatat
     6541 caacgttttc gatgctcttt tgtttgatca cttccagcga gtcaccaaag ccaccgacac
     6601 tgataatgac gataaccggc gtcaggatca gggcaaaaat catcaggctg gcctgtacag
     6661 tgtcagtcca gctcaccgcg aggaaaccgc caataaaggt gtaaaggatc gtcgccgcag
     6721 ctcccgccca cagagccgtt tcgtagctca tgccaaaggt actttcaaac agacgcgcgc
     6781 ctgccacaat gcccgaagcg caataaatgg tgaagaacag caaaatcacc agcgcggaga
     6841 taatgcgcaa aatgcggctt ttatcttcaa agcgcccggt gaaataatcc ggcaatgtta
     6901 aggcgttatt gttgtattcg gtatgcacac gcaaccgccc cgccaccagc ttccagttga
     6961 tccacgcgcc taatgtcagg ccaatggcga tccaactttc ggaaatcccg gaaagaaaaa
     7021 ccgcgcccgg caaccccatt aacagccagc cgctcatatc cgacgcaccc gccgataatg
     7081 ccgtcacgaa tggcccaagg ctacgaccgc ccagaatata gtcgtcaaag tttttcgttg
     7141 atcgccaggc gataaaccca atcaatatca tgccaaagat atagacacaa aatgtcacca
     7201 acatcggtgt gctaatagcc atctaaagtc tccaaaaaat tattatcggc aatgtcgaaa
     7261 cttgccgtta tatctgccac cggaacgggg taacagagtt tatgttttac cagggcgacc
     7321 gtatcctgcc ggaagcgctg gttattcaca atcgatttaa cacaccattt acattaaatt
     7381 ttagtgctca gcgacactat ttttcatcag gttgcactct ctcacatttt ttgcggttgc
     7441 acctttcaaa aatgttaact gccgcagaga aaaagtctga gttatttttt taatccctgt
     7501 catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc atgtcacatt
     7561 taacatggtt gcacaaagtt gcaacatcat ggatatttca cgataacgtt aagttgcacc
     7621 tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa gctggacgac
     7681 gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacgcc acactggtta
     7741 attaagcagg cgattttttc ttatctcgaa caactggaaa acagcgatac tctgccggag
     7801 ctacctgcgc tgctttctgg cgctgccaat gagagcgatg aagcaccgac tccggcagag
     7861 gaaccacacc aaccattcct cgactttgcc gagcaaattt tgcccctgtc ggtttcccgc
     7921 gccgcgatca cagcggccta tcgccgcccg gaaaccgaag cggtttctat gctgctggaa
     7981 caagcccgcc tgccgcagcc agttgctgaa caggcgcaca aactggcgta tcaactggcc
     8041 gataaactgc gtaatcaaaa aaacgccagt ggtcgcgcag gtatggttca ggggttattg
     8101 caggagtttt cgttgtcatc gcaggaaggc gtggcgctga tgtgtctggc ggaagcgttg
     8161 ttgcgtattc ccgacaaagc cacccgtgat gcgttaattc gcgacaaaat cagcaacggt
     8221 aactggcaat cacacattgg tcgtagcccg tctttgtttg ttaatgccgc cacctggggg
     8281 ctgctgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc ccgctcgctg
     8341 aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg
     8401 cgcctgatgg gcgagcagtt cgtcactggc gaaaccatcg cggaagcgtt agccaatgcc
     8461 cgcaagctgg aagagaaagg tttccgttac tcttacgata tgcttggcga agctgcactg
     8521 actgccgcag atgcacaagc gtatatggtt tcctatcagc aggcgattca cgccattggt
     8581 aaagcgtcca atggtcgtgg catctatgaa ggtccgggca tttcaatcaa gctgtcggcg
     8641 ctgcatccgc gttacagccg cgcccaatat gaccgggtaa tggaagagct ttacccacgt
     8701 ctgaaatcac tcaccctgct ggcgcgtcag tacgatattg gtatcaacat tgacgccgaa
     8761 gaggccgatc gtctggagat ctccctcgat ctgctggaaa aactctgttt cgagcctgag
     8821 ctggcaggct ggaacggtat cggttttgtt attcaggctt atcaaaaacg ctgcccactg
     8881 gtgattgatt acctgattga tctcgccacc cgcagccgtc gccgtctgat gattcgcctg
     8941 gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc
     9001 tatccggttt atacccgcaa ggtgtatacc gacgtttcat atctcgcctg tgcgaaaaag
     9061 ctgctggcgg tgccgaatct aatctacccg cagttcgcga cgcacaacgc ccatacgctg
     9121 gcagcgattt atcaactggc aggtcagaac tactatccgg gtcagtacga gttccagtgc
     9181 ctgcatggta tgggcgagcc actgtatgag caggtcaccg ggaaagttgc cgacggcaaa
     9241 cttaaccgtc cgtgtcgtat ttatgctccg gtcggtaccc atgaaacgct gttggcgtat
     9301 ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg tattgccgac
     9361 acctctttgc cactggatga actggtcgcc gatccggtca ctgctgtaga aaaactggcg
     9421 caacaggaag ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg cgatctttac
     9481 ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg gctggcctcg
     9541 ctctcttctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc aatgctggaa
     9601 caaccggtag cggcaggtga gatgtcgccc gttattaacc ctgcggaacc gaaagatatt
     9661 gtgggctttg tgcgtgaagc cacgccgcgt gaggtagaac aggcgctgga aagtgcggtt
     9721 aataacgcgc caatctggtt tgccactcct ccggttgaac gcgcggcgat tttgcatcgc
     9781 gctgccgtgc taatggaaag ccagatgcag caactgattg gtattctggt gcgtgaagcc
     9841 ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt tctccactac
     9901 tatgccggac aggtgcggga tgatttcgct aacgaaaccc accgtccatt agggcctgtg
     9961 gtgtgtatca gtccgtggaa cttcccgctg gctattttca ccgggcagat tgctgccgca
    10021 ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct gattgccgcg
    10081 caagggatcg ccattttgct ggaagcgggt gtaccgccag gcgtggtgca gttgctgcca
    10141 ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtacg cggggtgatg
    10201 tttaccggtt caaccgaagt cgctacgtta ctgcagcgca atatcgccag ccgcctggac
    10261 gctcagggtc gccctattcc gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc
    10321 gattcttcag cactgaccga acaggtcgtc gtggatgtac tggcctcggc gttcgacagt
    10381 gcgggtcagc gttgttcggc gctgcgcgtg ctgtgcctgc aagatgagat tgccgaccac
    10441 acgttgaaaa tgctgcgcgg cgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg
    10501 accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga gcgccatatt
    10561 cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat
    10621 gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact ggatgacttt
    10681 gccgaattgc aaaaagaggt ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac
    10741 cagctaccag agctgatcga gcagattaac gcttccggct atggtctgac gcttggtgtc
    10801 catacgcgta ttgatgaaac catcgcccag gtcactggct cggcccatgt tggtaacctg
    10861 tatgttaacc gtaatatggt gggcgcagtg gtgggtgtgc agccattcgg cggcgaaggg
    10921 ttgtccggta ccgggccgaa agcaggcggt ccgctctatc tctaccgtct gctggcgaat
    10981 cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg atgcagagta tccggtcgat
    11041 gcacagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat
    11101 cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa
    11161 cgattgctgc cggggccgac gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc
    11221 gtgttgtgta ttgccgatga tgagcaggat gcgctgactc agctcgccgc cgtgctggcg
    11281 gtgggcagcc aggtactgtg gccggatgac gcgctgcatc gtcagctagt aaaggcattg
    11341 ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg aaaatataac cgctcaaccc
    11401 tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt
    11461 gctgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgtggcga aagcaatatc
    11521 cttctggaac ggctgtatat cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt
    11581 aacgccagtt taatgactat aggttaaaca ggtagccgga ggatgttaca acctcctccg
    11641 gcatctttaa cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt caaccgtttg
    11701 attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa
    11761 atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc aaatgctgcg gatcaatcgg
    11821 cgcgagtttg ccgcttttga cccaaccggc aatcagcgcc gatttctcat caattaatgc
    11881 cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca tctccataca
    11941 gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc agcttcagac ggatgtactc
    12001 ttttatcgcc gccagcggag cgaaatcttc acgaaacgct tttaacggtg ccagccagat
    12061 atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa agtaatacag
    12121 cagattggtt tttgaaacac ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa
    12181 accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct tcgcgcttac
    12241 tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt
    12301 gtagattggc aacaggatag caaaaccccc gcccggcctc gaccttctgc actctcatcg
    12361 cgcacagatg catgttttat gtgcaactgt tttgaccgtt tagtccactt tttaccagat
    12421 attttagaca acacaatgtt aacacattaa taacattcac tttttaaaaa ctggcatccg
    12481 ctttgcaaac aagccattac gcggctgaaa aggatatgca ggatgcagcg ccccgtctga
    12541 ctttcacttt acgagacgaa gagaggttga tgatgaaaat tggcgtattc gtacctattg
    12601 gcaacaacgg ctggctcatt tcgacccacg cgccgcagta catgccgacc tttgaactga
    12661 ataaagccat tgtgcaaaaa gcggagcact accatttcga tttcgccctg tcgatgatca
    12721 aactgcgtgg ctttggcggc aaaactgagt tctgggatca caaccttgag tcgttcacct
    12781 tgatggcggg gctggcggcc gtcacctcgc gcattcagat ttacgccact gctgccacct
    12841 taacgttacc tccggcaatc gtcgcccgta tggccgcaac catcgactcc atctctggcg
    12901 ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc cgagtatgag cagatgggta
    12961 tctggcctgg cgatgactat ttctcccgtc gttacgacta tctcaccgaa tatgttcagg
    13021 tgctgcgcga cctgtggggc acggggaaaa gcgattttaa aggcgatttt ttcaccatga
    13081 atgattgtcg cgtcagtccg caaccgagtg tccccatgaa agtgatctgc gccgggcaaa
    13141 gcgacgctgg catggcgttc tccgcccagt atgccgattt caacttctgt ttcggcaaag
    13201 gcgtaaatac gcccacggct ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc
    13261 aaaccgggcg cgacgttggt tcttatgtgt tgtttatggt gattgccgat gaaaccgacg
    13321 atgccgctcg cgccaaatgg gaacactaca aagcgggcgc ggatgaagag gcgttaagct
    13381 ggctaaccga acaaagtcag aaagataccc gctccggtac tgataccaac gtccgtcaga
    13441 tggccgatcc cacttcggcg gtaaacatca atatggggac gttagtcggt tcttacgcca
    13501 gtgtcgcgcg catgttagat gaagtcgcaa gcgtgcctgg tgccgaaggc gtgctgttga
    13561 ccttcgatga ttttctgtcg ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc
    13621 agtgccgcgc ccatctacct gcgctgactc aggaggtggc atgatgacaa ccttaaccgc
    13681 tcgaccggaa gccattacct tcgatccgca gcaaagtgcg ctgatcgtgg tggatatgca
    13741 aaacgcttat gccacgccag gcggctactt agatctcgcc gggtttgatg tctcaaccac
    13801 tcgcccggtc attgccaaca ttcaaaccgc cgtgaccgca gcgcgagcgg cagggatgct
    13861 gatcatctgg tttcaaaatg gctgggatga acagtatgtc gaagctggcg gccccggctc
    13921 accgaatttt cataaatcga acgccctgaa aaccatgcgt aagcagccgc agctgcaggg
    13981 gaaattgctg gcgaaaggct cctgggatta tcaactggtg gatgaactgg tgccgcagcc
    14041 tggcgatatt gtgctgccga agccgcgcta cagcggtttc ttcaatacac cgctggacag
    14101 cattttgcgc agccgtggaa tacgccatct ggttttcacc ggtatcgcta ccaacgtctg
    14161 cgtcgaatcg acgctacgcg atggcttttt tctggagtat ttcggcgtgg tgctggaaga
    14221 cgcaacacac caggcggggc cggaatttgc acagaaagcc gcgttgttca atatcgaaac
    14281 cttttttggc tgggtcagcg acgtcgaaac gttctgcgac gcgctttctc ccacatcctt
    14341 tgctcgtatc gcttaaggag tttaacgatg ccaaaatccg taattattcc cgctggcagc
    14401 agcgcaccgc tggccccctt cgttcccggc acgctggctg atggcgtggt gtatgtctcc
    14461 ggtacgctgg cttttgatca acataataac gtgctgtttg ccgatgaccc aaaggcgcaa
    14521 acccgccacg ttctggaaat tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg
    14581 gatgtgacct tcaacagcat ctttattacc gactggaaaa attacgccgc gattaacgaa
    14641 atctacgccg agttttttcc gggtgataaa ccggcgcgat tctgcattca gtgcggactg
    14701 gtaaaacctg acgcgctggt ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc
    14761 gatgaaactt tcactctcac ctccccctta tgctgatacg cccgtagtgg tgttgatttc
    14821 gggtcttggc ggtagcggca gttactggtt accgcaactg gcggtgctgg agcaggagta
    14881 tcaggtagtc tgttacgacc agcgcggcac cggcaataat cccgacacgc tggcagaaga
    14941 ttacagtatc gcccagatgg cagcggaact gcatcaggcg ctggtagccg cagggattga
    15001 gcattacgcg gtggtcggcc atgcgctcgg tgcgctggtg ggaatgcagc tggcgctgga
    15061 ttatcccgcg tcggtaactg tgctgatcag cgttaacggc tggctacgaa taaacgccca
    15121 tacgcgccgc tgttttcagg ttcgcgaacg attactgtat agcggcggcg cgcaggcatg
    15181 ggtggaagcg cagccgttgt tcctctatcc cgccgactgg atggcggccc gcgcacctcg
    15241 cctggaggca gaagacgcgc tggcactggc gcattttcag ggcaaaaata atttactgcg
    15301 tcgacttaac gccctcaaac gcgctgactt tagtcaccat gcggatcgca tccgctgccc
    15361 ggtgcaaatc atctgcgcca gtgatgatct gctggtgcca tcagcatgtt ccagtgagct
    15421 tcatgccgcc ctgcccgata gccagaaaat ggtgatgccc tatggcggac acgcctgcaa
    15481 cgtgaccgat cccgaaacgt ttaatgctct gttactcaac gggcttgcca gcctgttaca
    15541 tcaccgtgaa gccgccctgt aaggaattgc tatgaacgaa gccgttagcc caggtgcgct
    15601 tagcaccttg ttcaccgatg cccgcactca caacggctgg cgggagacac ccgtcagcga
    15661 tgagacgtta cgggagattt atgccctgat gaaatggggg ccgacatcag ctaactgttc
    15721 tccggcacgg atcgtgttta tccgcacggc agaaggaaaa gaacgtctgc gcccggcact
    15781 ttccagcggc aatctgcaaa aaaccctgac cgcgcccgtc accgctatcg tcgcctggga
    15841 cagtgaattt tatgaacggt taccgcaact gtttcctcac ggcgatgccc gcagttggtt
    15901 tacctccagc ccacaacttg ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc
    15961 ctatctgatc gtcgcctgcc gtgcgctggg actggatacc ggcccgatgt cgggttttga
    16021 ccgtcaacac gttgacgacg ccttttttgc gggcagcacg ctgaagagca atctgctgat
    16081 taatatcggc tatggcgata gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga
    16141 agaagcctgc gggctgttgt aaggagcaaa ccatgaacat tgtcgatcaa caaacctttc
    16201 gcgatgcgat gtcctgtatg ggcgcggcag tcaatatcat caccacggat ggtccagccg
    16261 ggcgcgccgg gttcaccgcc agcgcggtct gcagcgtgac cgacacgccg cctacattac
    16321 tggtgtgcct gaatcgtggg gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt
    16381 gtgtaaatac actgagcgcc gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc
    16441 ccatggaaca ccgctttgcc gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac
    16501 tggaagaggc tctggtttcg tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc
    16561 acgacattct gttttgcgcc atcgaagcga ttcatcgtca cgccacaccc tacgggctgg
    16621 tgtggttcga tcgcagttat cacgcgctga tgcgccctgc ttgttaacct cctaaaggag
    16681 acagctgatg gcaatgttcg gttttcctca ctggcagtta aaatcgacct ctacagagag
    16741 cggcgtggtc gcgccgtatg aacgattgcc gtttgctcag acggcagtaa tgggcgttca
    16801 acacgcggtg gcgatgtttg gcgcgacggt gttaatgccg attctgatgg ggctggatcc
    16861 caatctttcc attttaatgt cggggatcgg cacgttactg tttttcttta ttaccggcgg
    16921 gcgcgtcccc agttatctcg gttccagcgc ggcttttgtc ggcgtggtga ttgccgcgac
    16981 gggctttaac ggtcagggca ttaacccgaa tatcagcatt gcgctgggcg ggattatcgc
    17041 ctgtgggctg gtttataccg tcattggcct ggtggtgatg aaaattggta cacgctggat
    17101 tgaacgacta atgccgccgg tggtgacggg tgcggtggtg atggcgattg gcctgaactt
    17161 agcgcctatc gcagtgaaaa gtgtatcggc ttcggccttt gatagctgga tggcggtaat
    17221 gacggtgctg tgtattgggc tggtggcggt gtttacccgt ggcatgatcc agcggttatt
    17281 gattctcgta ggtttaattg tcgcctgtct gctgtatggc gtgatgacca accttttagg
    17341 gctgggaaaa gccgtcgatt ttactctcgt cagccatgct gcctggttcg gtttgccaca
    17401 tttttccact cctgctttta atagtcaggc aatgatgctg attgcgcccg tggcggtgat
    17461 tctggtggcg gaaaacttag gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat
    17521 ggacccgtat atggggcggg cattcgtcgg tgatgggctg gcgacgatgc tttctggttc
    17581 tgtcggtggc agcggcgtaa ccacctacgc cgagaatatc ggtgtaatgg ccgtgacaaa
    17641 agtctactcg acgctggtgt ttgtcgctgc tgccgtcatc gccatgctgt tgggcttttc
    17701 accgaaattt ggcgcgctga ttcataccat tccggccgcg gtgattggtg gtgcatcaat
    17761 tgttgtgttc gggctgattg ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga
    17821 tttaagccag aacggtaatt tgattatggt cgcagtaacc ttagtgctgg gcgcgggtga
    17881 ttttgctctc acgctgggcg gttttacgtt gggagggatt ggtacagcaa cctttggcgc
    17941 gattttactc aatgcgttgt taagcagaaa gttggttgac gttccaccgc cagaagttgt
    18001 tcatcaggag ccataaacag aaaaccgccg ggattaccgg cggtgatgac tttttgacga
    18061 gcctaagaaa aaaatgcatt cgtcattgat tagcccggct agttgtcgct tttgccgtga
    18121 ctgctcttgc cacctttttt acctgcttca gatgcgcgct gcgggtcatt cttgaaatta
    18181 cccccgctgt gctgtccacc ttttttacct gcttctgatg ctctttcgcg gtcttctgca
    18241 aaattgccgg aaccgcctcg atggtttgcc attactgacc tccgtctggg tatagacatc
    18301 atattgcata gaattaacgc ggcatatggc ggctgccata ttttcgctac gtgggactaa
    18361 gcttagtgaa ctgacgcggg cagacccgcg tttggtaata tcctgcaaca gcttcttagc
    18421 gagaatagat atcatctcaa ataattatcc ataagccgct cttatgatta aaaaaacgcg
    18481 atgagataaa aatgtgtcat tttgcgacaa aattacgtgc ttgtgaaagt tgttataaat
    18541 caaataagtg gttgtgaaat ttgcactctg aaaaggacgt cttatcttta aataagtggt
    18601 agcgaatcgc tacggaatag agataacacg aggagtggtt agaaatggct aaagttctgg
    18661 tgctttatta ttccatgtac ggacatattg aaacgatggc acgcgcagtc gctgagggtg
    18721 caagcaaagt cgatggcgca gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc
    18781 aattatttga aaaagcaggc ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac
    18841 tggccgatta cgacgccatt atttttggta cacctacccg ctttggcaac atgtccggtc
    18901 aaatgcgtac cttcctcgac cagacgggcg gcctgtgggc ttccggcgca ctatacggaa
    18961 aactggcgag cgtctttagt tccaccggta ctggcggcgg tcaggaacaa actattactt
    19021 caacctggac gacccttgcg catcacggca tggtaattgt ccccattggc tacgcagcgc
    19081 aggaattatt tgacgtttca caggttcgcg gcggtacgcc gtacggcgca accaccatcg
    19141 caggcggtga cggctcacgc cagccaagcc aggaagaact gtctattgct cgttatcaag
    19201 gggaatatgt cgcaggtctg gcagttaaac ttaacggcta atcttcaaca ggaggatacg
    19261 catgccaact caagaagcga aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac
    19321 gtcgccggaa atagccgagg ccattttcga agtcgccggg tacgacgaaa aaatggcgga
    19381 aaagatttgg gaagaaggta gcgatgaagt gttagtcaaa gcctttgcta agaccgataa
    19441 agattcgctt ttttggggcg aacagaccat cgaacgtaaa aacgtttaac cagcgactcc
    19501 cccacttctc gcgggggagt tttctgttat ttcaccgcct cattcaacac gctatcaaac
    19561 ttatccattg ggcagaaacc gttggcatct atcgggcaac cgcttaattc cagcgtcaca
    19621 cgctgagcag gtgcctgcag ggttaacgca tcggcattac gtaactgttc cgcactctga
    19681 tacacatatt caattttcat caaatcgcga ttggctttgc tgtcatgcca acgctggaaa
    19741 acgattttgc cgccaatcgg cgtgcgttcg ttctggtcat gcaactgata cggtttgaaa
    19801 tccagcgccg ttaacagaga ggcaatgttg gagtcgtgcc caaccaacac tgtaattttc
    19861 ggtgcgctgg tgcgatcggt gaccagagct ttgtcgatat aactgaccag cggtttcgca
    19921 acattgcgcg ccacttccgg tgaggtaaac aggctgtcct ggtagccgtt tttaagcttc
    19981 gacaacacct tccactgctg gtcagatttg atttctcccc aggccacctg atccatcgga
    20041 aaaccttcgt aatattgcaa agtaaacgca tctaccagcg agttgccgac tttcagcggc
    20101 ccggaaacac ctggttcttg ttgatacttg gcgctaaagg tatttttgcc atccaccagc
    20161 gaacactgtt gtttctcttt acaggcaggg gaatctttat agttaacgat tttttccagt
    20221 agttggtagc tgtcggtaag ctggagtttg ctgagctctt tctccattgc cgccaccgcc
    20281 tgttcactga atgcggcgga atcatcggtg atcaccgggt taaaggttgg gtccatagtg
    20341 cccatttttt cctggtgatg cacaggaata tcacaccccg ggaatgcgcc ggtaataaag
    20401 aactgtgcgg tcgcaacggt acgttgcaga ctattggcat aggcataaac ggtgtccggc
    20461 ggcgggcatt cccccgattt caccatcccc tgctgtgcca gccattcacg catgtaatgg
    20521 cccatataca cttcgagcac gccacctttg gtggtgagtt gcccaccggg gacgtcccat
    20581 tcgggccatt tattcggcgt agactgctcc agcacactgc cattgttcgc cagcggcgca
    20641 cgtaagttat ggcggctcat catgagcact tgctgtagct gatagccttc cggtacggtt
    20701 tgtgcctgag catttgaagc gagtaaaact atccctgcca cagttgcggc gattagcgtt
    20761 ttgttcattc ctggcacctc ttttgttatc aatcactaaa gagtgtgaca gaaatatgac
    20821 atttttccgg gaacaggttc gcaaaatggc ttgttgaatg cctgtttttc tatatacatc
    20881 tttaaaaaca ggcaaggttt gacgcaaact atgcctgatt agctccctgc ctcgcgtacg
    20941 ccgctgatga ttacacctgt ggctaaacag aatatcagcg gcgttactaa tacagaggca
    21001 atagtaccaa taataagaga agtgaggcta ataacaatgg tttttagcac atgcctttgt
    21061 cagtaaattg cataacgagt taaaaaatca atttgcgcat tcctttcctt attgtttaac
    21121 tcgtaagcct gagcaaaaag ttcaggaggg aggtaaacgt cataatgatt gccagtgttt
    21181 attaaatacc catactcaag gtcgtcatct gttgaaaagg attcattccc taaggagatt
    21241 gatgccacat taccattaac aataaataat gagcaaatgc tatctgaaag agcagtgagg
    21301 aaatttttca cctcattaac cttctgctct gggtcggtgt ctaaaacaag gcgtgcctta
    21361 ttatcattaa atataaactc gggtaaatgc agtatgcatt ttttgtcatt caagcaacta
    21421 tcaataaatt cttttttaaa agacaaaaaa ctccatgata aaaaaccacc ctgcccctga
    21481 attttgttgt cagattgtag attaacgccg aataatgtct ttacatcttc cctgagatca
    21541 ttgaatgccg tatcaacaag agcctcgatc gtttgtttat caacaatttt aataaactct
    21601 tcgtatactt gcttttgcgt cttatggctg cccagcgccc atgaatactc atgttctttt
    21661 aaaaccagta tggcccttaa cagacaatag ccatcgccat ctactggagc gtattttaat
    21721 ccataagttt tattaatttc actctgaata aaagatatac cagacaaatc aataccagta
    21781 tctaaatcag atattcccgg cgtatttatc cattctggct ggatactcat attttcatca
    21841 tctaaacaat actcagaaaa aacatcatca cacgcattaa tgttttcaat atattccata
    21901 aagccatacc aataataacc tttaaaatct gtgatagact ctgaacctct gcccggcaag
    21961 gacactctaa caatatcaat atctcgaaca tattgtatag tatatgtaat accttctgaa
    22021 acccctgaat aagtttctgt atcattagta cgcaatgttc tcaaaaataa tgataaatca
    22081 ttatcattct gcatttcctc aaattctttt tctagtgaat cgcattccag gcatggaatg
    22141 tagtcttcag ctttttgacg tctgatttct ttatacaacc gcataaaaca caccagtgtg
    22201 ttttcaaaaa gcgtgattga atatttttga tttttttcgt gctcattttt tatattgttc
    22261 caggcttgtt tcaaataatt atttgcagtg caactaattc catgtatatt actactcata
    22321 tatagcgcct ataaaattta ataaataatg acgccccagt taaacttaaa gtgcctggtt
    22381 ccactatcaa aaatcgctca ccctttttca cctgtttaaa atatgttcag caccccacct
    22441 tgaggcgacc tcctctccgc gatgatttca ataacatatt ctgtgttggc atatgaaatt
    22501 ttgaggatta ccctacactt ataggagtta ccttacaggg gttccttcaa tttgtgttga
    22561 tttacgcgag ataacgctat ggaattaaag gattattacg ccatcatggg cgtgaaaccg
    22621 acggacgatc tcaagacaat caagaccgcc tatcgtcgac tggcccgcaa ataccatcct
    22681 gatgtcagca aagaaccgga tgccgaagcc cgcttcaaag aggtcgctga agcctgggaa
    22741 gtgttaagtg atgaacaacg tcgcgctgag tatgatcaga tgtggcaaca tcgcaacgat
    22801 ccgcaattta accgtcagtt ccatcatagc gacggtcaga gttttaacgc cgaagatttt
    22861 gacgatatct tctcgtcaat tttcggtcag catgcccgcc agagccgtca acgccccgcc
    22921 acacgcggcc acgatattga aatcgaagtg gcggtattcc tcgaagaaac gcttactgag
    22981 cataagcgta ccatcagcta taacctgccg gtttataacg cctttggcat gatcgaacag
    23041 gaaattccga aaacgctgaa tgtcaagatc ccggcgggcg tcggcaatgg tcaacgcatc
    23101 cgtctgaaag gccaggggac gccgggcgaa aacggcggtc caaatggcga tttgtggctg
    23161 gtgattcata ttgcgccaca tccgctgttc gatattgtcg gccatgatct ggaaattgta
    23221 gtgccggtta gcccgtggga agcggcgctg ggtgctaaag tcaccgttcc aacactgaaa
    23281 gaaagcattt tgctgactat cccgccaggc agccaggccg ggcaacgatt gcgcgttaaa
    23341 ggcaaaggtc tggtgagcaa aaaacagacc ggcgatctgt atgcggtact gaaaatcgtg
    23401 atgccgccga aaccggatga aaacactgcc gcgctgtggc agcaactggc agacgcccag
    23461 tcgtcttttg atccacgtaa agattggggg aaagcataat ggctaatgtt acggtgactt
    23521 ttactattac cgaattttgc ctgcataccg gcatctctga agaggagttg aatgaaattg
    23581 tcggtttggg ggtggttgaa ccgcgtgaga tccaggaaac aacctgggta tttgacgacc
    23641 atgccgccat cgtggtgcaa cgcgcagtac gcctgcgtca tgaactggct ctggactggc
    23701 ctgggatcgc ggtggcgctg acgttaatgg atgatattgc gcacctgaag caggaaaacc
    23761 gcctgctgcg ccagcgactt tcccggtttg taactcatcc gtgatgtgtg tttgccggat
    23821 gtgatgacgc atccggcaac tgccccataa tgcctgatgc gacgctaaac gcgtcttttc
    23881 aggcgcacaa acgacgttat ctgttttggt ggtcgctctc cactaacacc agcaataact
    23941 ggcttagtgc cgcgtaaaaa ccaaagctgt catactgacg acaacgcaca acaaactctg
    24001 gtaaccattg ccacagcgcc gtcagtgttt tttgccgcaa actgtcgatt cttcgcgcag
    24061 gaacggtccc ctctcccagc gaaaaatgca gatggctgag caattcgaga tagatcgcca
    24121 gatgatctgc cggttcgttg aaattgccgc tggtttccat ccctgcctca actaacaagc
    24181 gtttaatctc ttgctcgtcc tgtttgtagg ccgatgcata cggcagcgcc gcttgtttgt
    24241 cggtcatcag aaacaggccg caaaagtcag cggccagttc cagacgggca tcgtcacgta
    24301 ctgtcagcgt ggcaatacag ttttccagct cattcaccgc cgcagccagc ggcggttcgc
    24361 ttttcagcaa cgaaaaccat tcagccatct gcgcactggc gatttgcgtc agttgttcat
    24421 cgtccagttc acgggagaac aactgcgcca gccaggcgta aacacaggca atctgttgtg
    24481 ctgtcagcgt ggtcatgatt tcacctgcga cgcgggaaca tattcgcact gcgccaccat
    24541 ctccacaggg ccgttaaacg ccgtcacctg ctccactgct ccattgtact tctcaatttc
    24601 caccagcgta gtgtgcgcac tggtcgcctg cgccagctgc gaagtaccga tgtcgatggt
    24661 cagcacgttg gggttaccgt atttgcacag cgcaccaggc tcgccgcctt tatctggatc
    24721 gtaccatgcc ccttcgtgaa ttcgtgccac gccgggtgca tagcggtcag aaactactgc
    24781 ccctgccaac acctgaccgc gagcgttaaa gacgcgtacc acatcaccgt tacgaatacc
    24841 gcgcgcgctg gcatcctgcg ggttaatgaa cactggctct ttacccgcta ccgtatattg
    24901 ctgacgcaga gtttccgact cacataactg cgagtgaagt cggaaatccg gatgcacaga
    24961 ttgcagatgc aacggatact tctgcgagcc gggcccaccg tgggagcgtt cgattttctc
    25021 aaaccacatc ggatgcccct gacaatcgtc gtagttcata tcggcgatgg ttttcgagta
    25081 gatctcaatc aggccactcg gcgtgcccag cggttcgaga tccggatctt cgcggaatgc
    25141 ctggtggcga acaaacatct gcggatggtc aaactcgacg tactctttgt tattccagaa
    25201 gtcatcaaac gctggcagat gaacgccgcg tcctttgcct tgttgtacac cttcctgcca
    25261 gatgcgtttc agccagccca tttcgtccag cccttcggta aaggcttctt cgcgattaaa
    25321 gcgacggcac agctcgcgga aaatatcaaa gtcgttgcgc gcctcgaact gcggcggcac
    25381 cacctgtttc atggcgataa tgccacggtt ggagtggttg ccgtactggt cgagatcgtt
    25441 acgctcaaac tgcgtggtcg caggcagcac gatatcggca aagcggcagg ttgaggtcca
    25501 ctggttatct atggcgataa ccgtttccag cttgcgccag ccttcaataa tgcggttgat
    25561 ctgctgatgg cgatggaatg ggttagttcc ggcaaaaata cacattttca gcggcggcag
    25621 ttttaccgat ttaccgttcc agttgatcac tttccccggt tcgaggatcg catcgataaa
    25681 acgggcaatc ggaatggtgc tgctgtagcc tttgtaatca ctgttgtcgt gaacaggcgg
    25741 aatcgacgta gagccggaga aaccactcag aataacgcct ttacgccccg gcgtgcctgc
    25801 gccgttatag tgccagccaa aaccaaagcc accacctggc aggccaattt gccccagcat
    25861 cgccgccaga accacaatca tccacgccca ctgttcaccg tgctgcatac gctgcacgca
    25921 ccagccagca ataatttgcg ttctgttcgc cgccatctgc cgcgccagcc cacgaatggt
    25981 ttcggcatca atgccggtca gtttttcagc ccatgcggca tctttcggct gaccgtcttt
    26041 ctcacccagc aggtacggca ggaactgctc aaaacccaca cagtagttag cgaggaagtt
    26101 tttgtcgtac aggttttcac tgtacagcgt atacgccagc gccagttgca gcggcacatc
    26161 agtttgcggg ttaaccgcaa tgtgcttcac atgctcgcgc cccagatact catgggtgga
    26221 tgtgacaacc ggatcgatgc tgatgacctc aatttcaccg gcggcgactt tcgcttttag
    26281 ctgcgcgtaa tattcataaa catcgtgatc cgggcaccac cagttcgctt gctggttttt
    26341 cagcaaatca gagccccaca gcacaatggt tttgctgttc tgcaatacca gcggccagga
    26401 ggtttgctgt tcatacactt ccattgagcc aaccacgcgc ggcaggatca cctgcgcagc
    26461 accggtagag taatctccgc ccgtaccaac gctattacca tgcaaggcaa tagctttcgc
    26521 cagcatcccc gaagcgttat ggaacatccc cgtcgattgc caaccactgg cggtcagcaa
    26581 ggcactcggc ccgtgagttt tctgcacgcg ttccagttct tcatagaaca tgtcgagggc
    26641 ttcatcccag ctcacgcgca caaaacggtt atcaccgcgc tgggaggtat cgctgagatg
    26701 gcgcttacgc agccagtcca cgcgtaccat cggataacga atacgcgccg cgttgtgtac
    26761 gtgatccggc aatccggcaa tcattttcga cggatattta tccagttcga acggttttgc
    26821 cgccacaaag cgaccatcct tcaccgtcgc gcggatagcc ccccagtgcg acccggtaag
    26881 aatgccctct ttcgagatga cagcgtcagt cgccgcttgc gccgcagtcg cacggcgcgg
    26941 cgttaacaat gacggcccca gcatcccggc gacggttaag ccgccgagtt gtgccagaaa
    27001 acgccgacgt gatgcctgaa agagatcgtt attgttcatt atttttcttc cttcttatcg
    27061 ccgtgagcct tacctgcggt gtcagacgca ttcatttgca gatatttcaa caaggtgcgt
    27121 tcttcacgtt tatcgagact ggtaaagcca atcatgccgt tgagcgtgcc gatccaaccg
    27181 ttagcgtcaa agtgggcgat ttccggtgca ccgtggcact ggttacaggt gccgttgtac
    27241 aacgaatccg cataagccca gatcggtttg atatcgttca ccatgtcgcc tttcttcatc
    27301 cacgcagtgg cctgcaactt gctccactcg gtattggtgt cggcaacggt ggttttctcc
    27361 agcgttttta cctgctgctg cacatcacca cgaatcgagg caacaaagat gcgtttacct
    27421 gggaattggg tgagtacacg ctgacgtccg gcgctttccg tccagccggt aatttcaatt
    27481 tgcagccagt cgccgtcacg tttaaggact ttcacttccg aagcaggcag cagagaacca
    27541 gaggcttctt tatcgccttt cgccgcataa attggcttaa tatcaataga gtacagcgtg
    27601 tcaccactgt cattagcact ggcgcgcagc tcatcgaact gcttacggaa gccgctactc
    27661 atatccggta actggtgggc aataccttta tgacagtcga tgcaggattg attatctttc
    27721 gctgccacct tcatctgacg tgccgcttca ggatgctgct tcgcatgatc catcgcatcg
    27781 tagttatggc aggagcggca ggttgccgag ttgttttctt tcattcgcgc ccattcacgc
    27841 tcggcaagtt ccgcgcgttt ggcttcgaat ttttcaggtg tatcaatgga gtgagcaata
    27901 aaggtctggt agatatcatt gctcgcttcc agtttgcgct tcaccatgcc tggaatatcc
    27961 ggcgggatat gacagtcatg gcattcagct cgcacgccgg aggcgttctg gaaatgcacc
    28021 gactgtttat attcttcata caccggttgc atactgtggc aactgacaca aaattcggtt
    28081 gtgctggtga ctttgatccc aacgtgtggc aatacaatca gcgcaatgcc aatcacaatc
    28141 ccaattgcga ccagcgccag taccgaccaa cgagcactgg gtcggcgtag cgcgttccag
    28201 agtttccgca taatagcccc tgtaaaatta tggtttagtg aagcgatctt aatgagcaaa
    28261 tatgaacagc ggcactggtc aggatgaacg gcttacggca gaatatgaac agatatgaac
    28321 agatatgaac agaatgagta aaaccctctg atgccacatc acattgttat tgttgaagat
    28381 gagccggtta cccaggcgcg attacaatct tacttcactc aggaggggta taccgtttcc
    28441 gttacagcga gcggtgccgg gctgcgggaa attatgcaga atcagccggt agatttaatt
    28501 ctgctggata tcaacttacc cgatgaaaat ggcctgatgt taacccgcgc cctgcgagaa
    28561 cgctcaacgg tggggattat tctggttacc ggacgcagcg atcggattga ccgtattgtt
    28621 gggctggaaa tgggcgcaga cgattacgtc accaaaccgc tggaactgcg cgaactggta
    28681 gtacgggtga aaaatctgct ctggcgaatc gacctcgcgc gacaagctca accgcacact
    28741 caggacaact gctatcgctt tgccggttat tgcctgaatg tgtcgcgcca tacgctggag
    28801 cgggatggcg agccgattaa actgacccgc gcagagtatg aaatgttggt ggcatttgtg
    28861 acgaatccgg gcgaaattct cagccgtgaa cgtctgctac gtatgctttc tgcgcgtcgg
    28921 gtggaaaacc ctgacctgcg caccgtcgat gtgttaattc gtcgtttacg tcataaactc
    28981 agcgcggatt tactggtgac gcaacatggt gaaggttatt tcttagccgc tgatgtgtgc
    29041 tgataaaaat agaccggacg aaatccccct ggtgacagcg agcggcgaat atgttcacgg
    29101 tcggcatttt tcggcgtcag aactaaaatc ggtgggctga cattatcaga caccgattgc
    29161 ccctgtaatt gcctgatggc ctgctcaacc gccagttccc cctgccagac catttgatcg
    29221 ctggcagcca taatcactct tccccgcttc agcccgcgat acacctgatg tgaaagataa
    29281 aacgacacca cggtaagcgg cgttttcagg ttacgccctt cacccattgc cgcctctgcc
    29341 gcaatggccg ttccggcaac gacgtcgatt tctgggtggc gttccagcat ctcctgcaac
    29401 aggttacgct ggatttcaat atcgttatca ccaagcgcaa tatcaacaat acgcaccggg
    29461 cttccggcaa tggctgcgcg aaaaccctcg accatctctt tactgccccc ggcattatcg
    29521 ggtccgggca tcaacagcac gttcagtggt ttaccgtgcg cccattgcac caaatatcgc
    29581 ccaggttgat agcccatctg aaaccagggt acaccaacgc ggcttttcac caggggatca
    29641 tcaagagcat ttaccagttc gatcaccggc agatttgcta cctgcttttg caggtcggga
    29701 aatgacgtag tgctactacc gagcaaaatg gcctctgcgc cccactgttt acactggtcg
    29761 atttgtgctt gctgggtagc caactggctg tagccgcctg cctccagcac ttttaaatcc
    29821 acaccatagc ggcgagctgc ctcctgcata ccatagttca acgataacca gtatgaatct
    29881 ttcaggctgg gataaagcgc gcacagtttc catgcgcgtt tggctttaag cggcgtagag
    29941 gcttgcaccg tgtaatgctg cgcatcatgc cagcgcaaca ggttatcagc cgaaaatgcc
    30001 gataacatga aaagggaaag aagtaaaaat agcagtacgc gcatgatagc ctcatcaata
    30061 ataaggcttt atgctagatg cattccgctt tgcgactcaa cctttttcac ctaaaggatg
    30121 acaaatacac acaaatattt taaaaatcat attgttacat gaattcatga ttaaataaca
    30181 gactgcaaaa tacgataaaa cactacatct agagtcacta ttaatcaatg agttagatgt
    30241 ggttagtgac ctacaacaga gcattagcgc aaggtgattt tttgtcttct tgcgctaatt
    30301 ttttgtcata aaaacatgtg gcagactttg tatatctctg ctttgccacg ccacaccatc
    30361 atgcattatg gctatgccta aacagaatca ataagaatag aacataaaaa atgacaactc
    30421 tactagacaa ttaactacaa aaatgatagc aattagttta tataatataa gacttcctat
    30481 caaaagttca tccatatgaa gacaaggaat ttattatgcc aatcataaag aactgcttat
    30541 catcaattag ta
//
LOCUS       DAZFKM010000052        30348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFKM010000052 DAZFKM010000000
VERSION     DAZFKM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..559)
                     /gene="ybiA"
                     /locus_tag="ACHKAY_002389"
     CDS             complement(77..559)
                     /gene="ybiA"
                     /locus_tag="ACHKAY_002389"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO5701057.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            679..2829
                     /gene="dinG"
                     /locus_tag="ACHKAY_002390"
     CDS             679..2829
                     /gene="dinG"
                     /locus_tag="ACHKAY_002390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO5701058.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2857..3819
                     /gene="ybiB"
                     /locus_tag="ACHKAY_002391"
     CDS             2857..3819
                     /gene="ybiB"
                     /locus_tag="ACHKAY_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO5701059.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3960..5045
                     /gene="hcxB"
                     /locus_tag="ACHKAY_002392"
     CDS             3960..5045
                     /gene="hcxB"
                     /locus_tag="ACHKAY_002392"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO5701060.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5112..5171
                     /gene="ybiE"
                     /locus_tag="ACHKAY_002393"
     CDS             5112..5171
                     /gene="ybiE"
                     /locus_tag="ACHKAY_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO5701061.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5276..6640)
                     /gene="rhlE"
                     /locus_tag="ACHKAY_002394"
     CDS             complement(5276..6640)
                     /gene="rhlE"
                     /locus_tag="ACHKAY_002394"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO5701062.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            6869..7540
                     /gene="cecR"
                     /locus_tag="ACHKAY_002395"
     CDS             6869..7540
                     /gene="cecR"
                     /locus_tag="ACHKAY_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO5701063.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            7543..8538
                     /gene="hlyD"
                     /locus_tag="ACHKAY_002396"
     CDS             7543..8538
                     /gene="hlyD"
                     /locus_tag="ACHKAY_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO5701064.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            8531..10267
                     /gene="ybhF"
                     /locus_tag="ACHKAY_002397"
     CDS             8531..10267
                     /gene="ybhF"
                     /locus_tag="ACHKAY_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO5701065.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10260..11393
                     /gene="ybhS"
                     /locus_tag="ACHKAY_002398"
     CDS             10260..11393
                     /gene="ybhS"
                     /locus_tag="ACHKAY_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5701066.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11404..12510
                     /gene="ybhR"
                     /locus_tag="ACHKAY_002399"
     CDS             11404..12510
                     /gene="ybhR"
                     /locus_tag="ACHKAY_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5701067.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12472..12882)
                     /gene="ybhQ"
                     /locus_tag="ACHKAY_002400"
     CDS             complement(12472..12882)
                     /gene="ybhQ"
                     /locus_tag="ACHKAY_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO5701068.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13015..13776
                     /gene="ybhP"
                     /locus_tag="ACHKAY_002401"
     CDS             13015..13776
                     /gene="ybhP"
                     /locus_tag="ACHKAY_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5701069.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            13773..15014
                     /gene="clsB"
                     /locus_tag="ACHKAY_002402"
     CDS             13773..15014
                     /gene="clsB"
                     /locus_tag="ACHKAY_002402"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO5701070.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15014..15970
                     /gene="ybhN"
                     /locus_tag="ACHKAY_002403"
     CDS             15014..15970
                     /gene="ybhN"
                     /locus_tag="ACHKAY_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO5701071.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16006..16719)
                     /gene="ybhM"
                     /locus_tag="ACHKAY_002404"
     CDS             complement(16006..16719)
                     /gene="ybhM"
                     /locus_tag="ACHKAY_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5701072.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(16924..17628)
                     /gene="ybhL"
                     /locus_tag="ACHKAY_002405"
     CDS             complement(16924..17628)
                     /gene="ybhL"
                     /locus_tag="ACHKAY_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO5701073.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(17765..18217)
                     /gene="moaE"
                     /locus_tag="ACHKAY_002406"
     CDS             complement(17765..18217)
                     /gene="moaE"
                     /locus_tag="ACHKAY_002406"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO5701074.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(18219..18464)
                     /gene="moaD"
                     /locus_tag="ACHKAY_002407"
     CDS             complement(18219..18464)
                     /gene="moaD"
                     /locus_tag="ACHKAY_002407"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO5701075.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(18457..18942)
                     /gene="moaC"
                     /locus_tag="ACHKAY_002408"
     CDS             complement(18457..18942)
                     /gene="moaC"
                     /locus_tag="ACHKAY_002408"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO5701076.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(18945..19457)
                     /gene="moaB"
                     /locus_tag="ACHKAY_002409"
     CDS             complement(18945..19457)
                     /gene="moaB"
                     /locus_tag="ACHKAY_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO5701077.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(19479..20468)
                     /gene="moaA"
                     /locus_tag="ACHKAY_002410"
     CDS             complement(19479..20468)
                     /gene="moaA"
                     /locus_tag="ACHKAY_002410"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO5701078.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(20456..20600)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            20865..21773
                     /gene="yvcK"
                     /locus_tag="ACHKAY_002411"
     CDS             20865..21773
                     /gene="yvcK"
                     /locus_tag="ACHKAY_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO5701079.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(21965..23986)
                     /gene="uvrB"
                     /locus_tag="ACHKAY_002412"
     CDS             complement(21965..23986)
                     /gene="uvrB"
                     /locus_tag="ACHKAY_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO5701080.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(24565..25242)
                     /gene="bioD"
                     /locus_tag="ACHKAY_002413"
     CDS             complement(24565..25242)
                     /gene="bioD"
                     /locus_tag="ACHKAY_002413"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5701081.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(25235..25990)
                     /gene="bioC"
                     /locus_tag="ACHKAY_002414"
     CDS             complement(25235..25990)
                     /gene="bioC"
                     /locus_tag="ACHKAY_002414"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO5701082.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(25977..27131)
                     /gene="bioF"
                     /locus_tag="ACHKAY_002415"
     CDS             complement(25977..27131)
                     /gene="bioF"
                     /locus_tag="ACHKAY_002415"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO5701083.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(27128..28168)
                     /gene="bioB"
                     /locus_tag="ACHKAY_002416"
     CDS             complement(27128..28168)
                     /gene="bioB"
                     /locus_tag="ACHKAY_002416"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO5701084.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            28255..29544
                     /gene="bioA"
                     /locus_tag="ACHKAY_002417"
     CDS             28255..29544
                     /gene="bioA"
                     /locus_tag="ACHKAY_002417"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO5701085.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            29603..30079
                     /gene="ybhB"
                     /locus_tag="ACHKAY_002418"
     CDS             29603..30079
                     /gene="ybhB"
                     /locus_tag="ACHKAY_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO5701086.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         7073 a         8056 c         8065 g         7154 t
ORIGIN      
        1 acaacaagca cttgtcagca atttgagaac acgggaaaaa atttatctgt tttaccgccc
       61 ataagccacc gagacgttac ttctctatag ccaattgttc gcgcaactcc attaaaaggt
      121 agcccagtcg attcttgccc ttaccattac caccgtctcc ccagtaagca tcgttttccg
      181 tatgctcaac cagttttgcg ggcgcggttg ccagcaagag cgcacgcagt tctggatgct
      241 gttcgaattt agcgcgaagt gcttttcgca tcacctgttc tttgaccgac tcccagtttt
      301 tacgcagagg cttagaacga tcgcgcccca tgcgtgcggc gaccataggg gaagaaaccc
      361 gacggatctc ttcgcggtat ttttcatcaa ggaatttttg tgcctgaaaa tagtgttctg
      421 aggtgggcca ggttttcccg tcaaccttga tgggccaggc ggcaaagtta gaaaaatcac
      481 cgtagtcatc gctggtgctg tagaaattta tgatggtgtc ttgcatgacg tgttggatcc
      541 tttgtgctcg aacgggcatt aaaccgcatt atgttggtgg ttattgcgag ccgctttcca
      601 gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc acaatattgg ctgtttatac
      661 agtatttcag gttttctcat ggcattaacc gccgcgctta aagcgcaaat tgccgcctgg
      721 tataaggcgc ttcaggaaca gatccccgac tttattcccc gtgcgccgca gcggcagatg
      781 attgcggacg tcgccaaaac gctggccgga gaagaagggc gacatctggc gattgaagcc
      841 cccaccggcg ttgggaaaac gctctcctat ttgatccccg gcatcgccat tgcccgcgaa
      901 gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcat tgcaggatca gatttacagc
      961 aaagatttac cgctgctgaa aaagatcatt cccgatctta aattcactgc cgcttttggg
     1021 cgtgggcgct acgtttgccc gcgtaatctg acggcgctcg ccagcactga gcccacgcaa
     1081 caggatctgc tggcgtttct tgacgacgaa ctgacgccaa ataatcagga agagcaaaaa
     1141 cgttgtgcga agctgaaggg cgatctcgac acttataaat gggatggtct gcgcgatcat
     1201 actgatatcg ctatagatga cgatctctgg cgtcgtttaa gtaccgacaa agccagctgc
     1261 ctcaaccgca actgttacta ctatcgtgaa tgcccgtttt ttgttgctcg tcgggagatt
     1321 caggaagcgg aagtggtggt ggcaaaccat gcgctggtga tggcggcgat ggaaagcgaa
     1381 gccgtactac ctgacccgaa aaatctgctg ctggtgctgg acgaaggtca tcacctgcca
     1441 gatgtggccc gggatgcgct ggagatgagt gccgaaatca ccgcgccctg gtatcgacta
     1501 cagctggact tgttcacgaa actggtcgct acctgcatgg agcagtttcg cccgaagacc
     1561 atcccaccgc tggcgatccc tgaacgtttg aatgcgcatt gtgaagagtt gtatgagctt
     1621 atcgcctcgt taaacaacat tctcaatctc tacatgcctg ccgggcagga ggcagagcac
     1681 cgttttgcga tgggcgaact gcctgatgaa gtgctggaga tctgccagcg gctggcaaaa
     1741 ctcaccgaga tgctgcgtgg tctggcggag ttatttctta acgatttaag tgagaaaacc
     1801 ggcagccacg atattgtgcg tctgcatcgg ttgattttgc agatgaaccg cgcgttgggg
     1861 atgttcgagg cgcaaagcaa actctggcgg ctggcgtctc tggcgcaatc ttccggtgcg
     1921 ccggtgacca aatgggcgac gcgggaagag cgcgaagggc agctgcatct ctggtttcac
     1981 tgcgtgggaa tacgtgtcag cgatcagctg gaaaggctgc tgtggcgcag tattccgcac
     2041 attattgtca cctccgcaac cttgcgttcg ctgaacagtt tttcgcgttt gcaggagatg
     2101 agtggtctga aagagaaagc gggcgaccgt tttgtggcgc tggattcccc ctttaaccac
     2161 tgcgaacagg gcaaaattgt tattccccgg atgcgctttg agccttccat cgacaacgaa
     2221 gagcagcata ttgccgaaat ggcggccttt ttccgtaagc aggtggagag caaaaaacat
     2281 ctcggtatgt tggtactgtt tgccagcgga cgggcgatgc agcgctttct cgactatgtg
     2341 acggatttac gcctgatgct gctggtgcag ggcgatcagc cgcgttatcg cttagttgaa
     2401 ctgcaccgca aacgcgttgc caacggtgag cgtagtgtgc tggtgggctt acagtcattt
     2461 gccgaagggc ttgatttgaa aggtgatctg ctcagccagg tgcatatcca caaaatcgct
     2521 tttccgccca tcgacagccc ggtggtgatc accgaagggg aatggctgaa aagcctcaac
     2581 cgctatccgt ttgaggtgca aagcctgccg agcgcctcgt ttaacctgat tcagcaggtt
     2641 gggcgactga ttcgaagcca cggttgctgg ggcgaagtgg ttatctacga caaacgcttg
     2701 ctgaccaaaa attatggcaa gcgactactg gatgcattac cggtatttcc gatagagcaa
     2761 ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa cgaaatcccc acgccgtcgg
     2821 cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg actatcgcaa aatcattaaa
     2881 gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc gggatactgc gcgcggtctg
     2941 tatgcccata tgctcaatgg tgaagtccct gaccttgagt tgggcggcgt attgattgcg
     3001 ctgcgtatca aaggggaagg ggaagcagag atgctcggct tttacgaagc catgcaaaat
     3061 cacaccatca agctgacgcc gcctgcaggc aagccgatgc cgattgtcat ccccagttac
     3121 aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg cgattctcct gcacaaactc
     3181 ggttttcctg tggtggttca cggggttagc gaagatccaa cccgcgtgct gactgaaacc
     3241 atttttgaat tgatgggtat tacgccaacg ttgcacggcg gacaggcgca ggcgaagctc
     3301 gacgaacatc aaccggtgtt tatgccagtc ggcgcgtttt gcccgccgct ggaaaaacaa
     3361 ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac ataccctggc gaaactggca
     3421 acgccatttg ccgaaggtga ggcgctgcgt ctttccagcg tttcgcatcc ggaatacatt
     3481 ggacgcgtcg cgaagttctt tagcgatatc ggcgggcggg cgctactaat gcatggtact
     3541 gaaggtgaag tgtatgctaa tccgcagcgc tgcccgcaga tcaatctcat tgaccgtgaa
     3601 gggatgcggg tgctgtatga aaaacaggac actgctggta gcgagttact gccacaagca
     3661 aaagatccgg aaaccacggc gcagtggatt gagcgttgcc ttgctggcag cgaaccgatt
     3721 cccgaatcgc tgaaaatcca gatggcttgc tgcctggtgg ctacgggtga agcggcaact
     3781 atcagcgacg gcctggcgcg cgttaatcag gcattttaat tcttttctca gccggatgag
     3841 ccatgtcatc cggcttttac cccaccgcaa tatgaaattc ctgcatcttt attgaccttc
     3901 ccacgcccgg cgtgcagcat aaaaatacaa caaacacata acataaacag gagttaacca
     3961 tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag ttttattcag gctgtatttc
     4021 gtcagatggg tagcgaggaa caagaagcga aattagttgc cgatcattta atcgcggcaa
     4081 acctggcagg gcatgattca catggtattg gcatgatccc aagctatgta cgctcctgga
     4141 gtcaggggca cctgcaaatt aaccatcatg ccaaaaccgt taaagagtcg ggggcggcgg
     4201 tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc acatgaagcg atggcgctgg
     4261 ggattgagaa agcgcataag cacggtattg ccgccgtggc gctacataac tcgcatcata
     4321 tcggccgtat cggttactgg gcggagcagt gtgcagcggc ggggtttgtc tctatccact
     4381 ttgttagcgt ggtcggtatt ccaatggtcg cgccgttcca cggtcgcgac agccgctttg
     4441 gcaccaatcc gttctgtgtg gttttccctc gtaaagataa tttcccgctg ttgcttgatt
     4501 acgccaccag cgccattgca tttggtaaaa ctcgcgtcgc ctggcataaa ggcgtccccg
     4561 tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac gaccaatccg gcggtaatgc
     4621 aggagtcgcc gttgggggcg ctgttgacct ttgccgaaca taaaggctac gcccttgcag
     4681 cgatgtgtga aattcttggc ggggcgcttt ctggcggtaa aacgacgcat caggaaacgt
     4741 tacaaaccag tcccgatgcc attcttaact gcatgaccac tatcatcatc aacccggaac
     4801 tcttcggcgc gccggattgt aacgcgcaga ccgaagcctt tgccgagtgg gtgaaagcct
     4861 cgccgcatga tgatgataag ccgattttgc taccgggcga gtgggaagtg aacacgcgtc
     4921 gcgaacggca gaagcagggg attccactgg atgcgggaag ctggcaggcc atttgtgatg
     4981 cagcgcggca gattggtatg ccggaagaga cgttgcaggc tttctgtcag cagttagcca
     5041 gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag ggtaacatag gatcaatgag
     5101 ggttagagca tatgcgtctg tcggcaaaca gacagggaaa tacttgtgct ggacgtagcg
     5161 taaacgcctg atccgtccaa cggttcggat tttgtagata ctgataagcg tagcgcatca
     5221 ggcagttttg cgtttgttca tcagcctgat gccgggcata gcccggcata aaagattact
     5281 gcgcagcggt aggtttacgc ggacggcggc gacgttgttg ttcgcctgcc ggtttggcat
     5341 cgccgaggcg gcgagacggt ttttctgcag gtttcgcgct tgcagatttt gcgccacctt
     5401 ccccacggcg tggttgctgt tgaccgcgac caccaccttg cccacgaccg ccgtcgccac
     5461 gttgctggcg accgttctgg atcggttcgg ctttgattga cgggtccggc tcatagcccg
     5521 gaatcgcaat gcgcgggatc tcttttttca gcagtttttc gatatcacgc agcagtttgt
     5581 gttcatcaac acacaccagc gacaacgctt caccggtagc agccgcacga ccggtacgcc
     5641 cgatacggtg gacataatct tccggtacgt ttggcagttc atagttgacg acgtgcggca
     5701 actcttcgat atccagaccg cgcgcagcga tatcggtcgc caccagtacg cgaatgtcac
     5761 ccgatttaaa atcagccagc gcacgggtac gcgcaccttg cgatttattc ccgtggatcg
     5821 ccgcactacg gatgccatct ttattgagct gctcagccag atggttagcg ccgtgtttgg
     5881 tacgggtaaa caccagcacc tgctgccagt tccctttacc aatcatgtgc gacagcaatt
     5941 cgcgtttgcg tttcttatca acaaagtgaa cgtgctgggt cacctgatca gacgcggtat
     6001 tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag tttttccgcc agggctttaa
     6061 tatcgtcaga gaaggtcgca gagaacagca ggttctggcg cttcgctggc agttttgtta
     6121 acacgcgacg gatatcgtgg ataaagccca tgtcgagcat gcggtcagct tcatcgagga
     6181 cgaggatttc aacctgatcc agcttcactg cattttgatg ttccaggtcc agcaaacgtc
     6241 ccggtgttgc caccagaaca tcaacgccgc cgcgcagctt catcatttgc gggttaatgc
     6301 tgacaccgcc aaacaccacc agcgaacgaa tgttcaaata tttgctgtaa tcacggacgt
     6361 tttcgccaat ctgcgcagcc agttcacggg tcggggtaag aatgagcgca cgtaccggac
     6421 gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg caacagcggc agcgtaaagc
     6481 ccgctgtttt accggtaccg gtctgggcgc tagccatcag gtcgcggcct tccagcaccg
     6541 cagggatcgc ctgctgctga ataggggtgg gttcacggta accctgctcg gcaacggcgc
     6601 gcaggatatc agggcttaaa cccaaagaat cgaaagacat aactactccg aaccgccccg
     6661 accgttaccg gtgtagtttt cagggagata ctgaaaagga aaatgacaaa aaccacaatg
     6721 tcatgaggga gcggagtgta gcagtttttg tgacgcagcg cataaattat ccctgtgact
     6781 ggcgtagcta aaattcttag ccatactgga caactcccat atggagtcat aatcttaatc
     6841 aatcatttga ttaagaatgg actgtgcgat gaataatcct gccatgacaa tcaagggtga
     6901 acaggcgaaa aaacagctga ttgctgccgc actggcgcag tttggtgaat atggaatgaa
     6961 cgccaccacc cgcgagatag ccgcccaggc cgggcagaat atcgctgcca tcacctacta
     7021 cttcggttcg aaagaagatt tgtacctcgc ctgcgcccag tggattgccg attttattgg
     7081 cgagcagttc cgtccgcacg ccgaagaggc cgaacgcttg ttcgcacaac cacagcctga
     7141 tcgcgcagcc atccgtgaac tgatccttcg cgcctgcagg aacatgatta agctgctcac
     7201 ccaggatgac accgtcaacc tcagcaagtt tatctcccgt gagcagctct ctcccacggc
     7261 agcctatcac ctggtgcacg aacaggtgat tagcccgcta cacagccacc tgacacgcct
     7321 gattgccgcc tggaccggct gcgacgccaa cgatacccgc atgatcctcc atactcatgc
     7381 gttgattggt gagattctgg cgttccgtct tggcaaagaa acaattctgt tacgtaccgg
     7441 ctggaccgcg ttcgatgaag aaaagaccga actgatcaac cagacggtga cctgtcatat
     7501 cgacctgatt ttgcaaggat tatcgcaaag gagtttgtag tgatgaaaaa acctgtcgtg
     7561 atcggactgg cggtagtggt acttgccgcc gtggttgccg gaggctactg gtggtatcaa
     7621 agccgccagg ataacggcct gacgctgtat ggcaacgtgg atattcgtac ggtgaatctt
     7681 agtttccgtg ttggggggcg cgttgaatcg ctggcggtgg acgaaggtga tgctatcaaa
     7741 gcgggccagg tgctgggcga actggatcac aagccgtatg agattgccct gatgcaggcg
     7801 aaagcgggtg tttcggtggc acaggcgcag tatgacctga tgcttgccgg gtatcgcgat
     7861 gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc aagccgccta tgactatgcg
     7921 cagaacttct ataaccgcca gcaagggttg tggaaaagcc gcaccatttc ggcaaacgac
     7981 ctggaaaatg cccgctcctc gcgcgaccag gcgcaggcaa cgctgaaatc agcacaggat
     8041 aaattgcgtc agtaccgttc cggtaaccgt gaacaggaca tcgcccaggc gaaagccagc
     8101 ctcgaacagg cgcaggcgca actggcgcag gcggagttga atttgcagga ctcaacgttg
     8161 atagccccgt ctgatggcac gctgttaacg cgcgcggtgg agccaggcac ggtcctcaat
     8221 gaaggtggca cggtgtttac cgtttcacta acgcgtccgg tgtgggtgcg cgcttatgtt
     8281 gatgaacgga atcttgacca ggcccagccg gggcgcaaag tgctgcttta taccgatggt
     8341 cgcccggaca agccgtatca cgggcagatt ggtttcgttt cgccgactgc tgaatttacc
     8401 ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg tctatcgcct gcgtattgtg
     8461 gtgaccgacg ccgatgatgc gttacgccag ggaatgccgg tgacggtgca attcggtaac
     8521 gaggcaggac atgaatgatg ccgttatcac gctgaacggc ctggaaaaac gctttccggg
     8581 catggacaag cccgccgtcg cgccgctcga ttgtaccatt cacgccggtt atgtgacggg
     8641 gctggtgggg ccggatggtg caggtaaaac cacgctaatg cggatgctgg cgggattact
     8701 gaaacccgac agcggcaatg ccacggtgat tggctttgat ccaatcaaaa acgacggcgc
     8761 gctgcacgcc gtgctcggct atatgccgca gaaatttggt ctgtatgaag atctcacggt
     8821 gatggagaac ctcaatctgt acgcggattt gcgcagcgtc accggcgagg cacgtaagca
     8881 aacttttgct cgcctgctgg agtttacgtc tcttgggccg tttaccggac gcctggcggg
     8941 caagctctcc ggtgggatga aacaaaaact cggtctggcc tgtaccctgg tgggcgaacc
     9001 gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac cctatctcac ggcgcgaact
     9061 gtggcagatg gtgcatgagc tggcaggcga agggatgtta atcctctgga gtacctcgta
     9121 tctcgacgaa gccgagcagt gccgtgacgt gttactgatg aacgaaggcg agttgctgta
     9181 tcagggggaa ccaaaagccc tgacacaaac catggccgga cgcagttttc tgatgaccag
     9241 tccacacgag ggcaaccgca aactattgca acgcgccttg aaactgccac aggtcagcga
     9301 cggcatgatt caggggaaat cggtacgtct gatcctcaaa aaagaggcca caccagacga
     9361 tattcgccat gccgacggga tgccggaaat caacatcaac gaaactacgc cgcgttttga
     9421 agatgcattt attgatttgc tgggtggtgc cggaacctca gaatcgccgc tgggcgcaat
     9481 attacatacg gtagaaggca cacccggcga gacggtgatc gaagcgaaag aactgaccaa
     9541 gaaatttggt gattttgccg ccaccgatca cgtcaacttt gccgttaaac gtggggagat
     9601 ttttggtttg ctggggccaa acggcgcggg taaatcgacc acctttaaga tgatgtgcgg
     9661 tttgctggtg ccgacttccg gccaggcgct ggtgctgggg atggatctga aagagagttc
     9721 tggtaaagcg cgccagcatc tcggctatat ggcgcaaaag ttttcgctct acggtaacct
     9781 gacggtcgaa cagaatttac gctttttctc tggtgtgtat ggcttacgcg gtcgggcgca
     9841 gaacgaaaaa atctcccgta tgagcgaggc gttcggcctg aaaagtatcg cctcccacgc
     9901 caccgatgaa ctgccattag gttttaaaca gcggctggca ctggcctgct cgctgatgca
     9961 tgaaccggac attctgtttc tcgacgaacc gacgtccggc gttgatccga ttactcgccg
    10021 cgaattctgg ctacatatca acagcatggt agagaaaggc gtcacggtga tggtcaccac
    10081 ccactttatg gatgaagcag aatattgcga ccgcatcggc ctggtgtacc gtgggaaatt
    10141 aattgccagc ggcacgccgg atgatttgaa agcgcagtcg gccaacgacg agcaacccga
    10201 tcccaccatg gagcaagcct ttattcagtt gatccacgac tgggataagg agcatagcaa
    10261 tgagtaaccc gatcctgtcc tggcgtcgcg tacgggcgct gtgcgttaaa gagacgcggc
    10321 agatcgttcg cgatccgagt agctggctga ttgcggtagt gatcccgctg ctactgctgt
    10381 ttatttttgg ttacggcatt aacctcgact ccagcaagct gcgggtcggg attttactgg
    10441 aacagcgtag cgaagcggcg ctggatttca cccacaccat gaccggttcg ccctacatcg
    10501 acgccaccat cagcgataac cgtcaggaac tgatcgccaa aatgcaggcg gggaaaattc
    10561 gcggtctggt ggttattccg gtggattttg cggaacagat ggagcgcgcc aacgccaccg
    10621 caccgattca ggtgatcacc gacggcagtg agccgaatac cgctaacttt gtacaggggt
    10681 atgtcgaagg gatctggcag atctggcaaa tgcagcgagc ggaggacaac gggcagactt
    10741 ttgaaccgct tattgatgta caaacccgct actggtttaa cccggcggcg attagccagc
    10801 acttcattat ccccggtgcg gtgaccatta tcatgacggt catcggcgcg attctcacct
    10861 cgctggtggt ggcgcgagaa tgggaacgcg gcaccatgga ggctctgctc tctacggaga
    10921 ttacccgcac ggaactgctg ctgtgtaagc tgatccctta ttactttctc gggatgctgg
    10981 cgatgttgct gtgtatgctg gtgtcagtgt ttattctcgg cgtgccgtat cgcgggtcgc
    11041 tgctgattct gttttttatc tccagcctgt ttttactcag taccctgggg atggggctgc
    11101 tgatttccac gattacccgc aaccagttca atgccgctca ggtcgccctg aacgccgctt
    11161 ttctgccgtc gattatgctt tccggcttta tttttcagat cgacagtatg cccgcggtga
    11221 tccgcgcggt gacgtacatt attcccgctc gttatttcgt cagcaccctg caaagcctgt
    11281 tcctcgccgg gaatattcca gtggtgctgg tggtaaacgt gctgtttttg atcgcttcgg
    11341 cggtgatgtt tatcggcctg acgtggctga aaaccaaacg tcggctggat tagggagaag
    11401 agcatgtttc atcgcttatg gacgttaatc cgcaaagagt tgcagtcgct gctgcgcgaa
    11461 ccgcaaaccc gcgcgattct gattttacct gtgcttattc aggtgattct gttcccgttc
    11521 gccgccacgc tggaagtgac taacgccacc atcgccatct acgatgaaga taacggcgag
    11581 cattcggtgg agctgaccca acgttttgcc cgcgccagcg cctttactca tgtgctgctg
    11641 ctgaaaagcc cacaggagat ccgcccaacc atcgacacac aaaaggcgtt actactggtg
    11701 cgtttcccgg ctgacttctc gcgcaaactg gataccttcc agaccgcgcc gttgcagttg
    11761 atcctcgacg ggcgtaactc caacagtgcg caaattgccg ccaactacct gcaacagatc
    11821 gtcaaaaatt atcagcagga gctgctggaa ggaaaaccga aacctaacaa cagcgagctg
    11881 gtggtacgca actggtataa cccgaatctt gactacaaat ggtttgtggt gccgtcgctg
    11941 atcgccatga tcaccactat cggcgtaatg atcgtcactt cactttccgt cgcccgcgaa
    12001 cgtgaacaag gtacgctcga tcagctactg gtttcgccgc tcaccacctg gcagatcttc
    12061 atcggcaaag ccgtaccggc gttaattgtc gccaccttcc aggccaccat tgtgctggcg
    12121 attggtatct gggcgtatca aatccccttc gccggatcgc tggcgctgtt ctactttacg
    12181 atggtgattt acggtttatc gctggtggga ttcggtctgt tgatttcatc actctgctcg
    12241 acacaacagc aggcgtttat cggcgtgttt gtttttatga tgcccgccat tctcctttcc
    12301 ggttacgttt ctccggtgga aaacatgcca gtatggctgc aaaacctgac gtggattaac
    12361 cctattcgcc actttacgga cattaccaag cagatttatt tgaaggatgc gagtctggat
    12421 attgtgtgga atagtttgtg gccgctactg gtgataacgg ccacgacagg gtcagcggcg
    12481 tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct ttcgccagca aagacactac
    12541 cgccgggccg gcaaggattg ccagccctgc aattgccagc aaaaagttgt tttccagtac
    12601 acgggacaac agccacagca caatcagcgc tgcgccaaaa taccaggtcg tggtcagctc
    12661 ttccagcacg tctgccacct cacgccagcg ttgctcgggg tggcgctgaa acaccaacat
    12721 catcactacc gtgacacaat acagtgcgca taatcccacg ccgacgtgaa ccagagtctt
    12781 actcatccag cccaccacca gcagcgccac taccagtaaa tgcaacataa tctgccagca
    12841 acttagacca gttgcgacac gaacacgttg ttgccacttc atggcttctc tcctgaagaa
    12901 tttatctcta cttactcaga gtaccgaact ttacggaaaa ttccgtaaga ccgcaccttt
    12961 ttgtgacgcc gactacacta tttcaatgag ataagaggca aagacaggaa aattatgccc
    13021 gatcaaacac aacaattttc gttcaaggtg ctcaccatca acattcacaa aggttttacc
    13081 gcgtttaacc gacgcttcat tttgccggaa cttcgcgacg cggtgcgtac cgtcagcgcc
    13141 gatattgttt gcctgcagga agtgatgggc gcgcacgaag ttcatccgct gcatgtggaa
    13201 aactggcccg atacctcgca ctacgagttt ctcgccgaca cgatgtggag cgattttgcc
    13261 tacggtcgca atgccgtata cccgaaaggg catcacggca acgccgtact gtcgcgttat
    13321 cccattgaac attatgagaa tcgcgatgtt tcggtcgatg gtgcggaaaa gcgcggcgtg
    13381 ctctactgcc gcattgtgcc accgatgacc ggaaaagcga ttcatgtgat gtgcgtacat
    13441 ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga tgctcgccga atgggtgaat
    13501 gagctaccgg acggcgaacc ggtattggtg gcgggtgatt tcaatgactg gcggcaaaaa
    13561 gctaatcatc cgttaaaagt gcaggccgga ctggatgaga tttttacccg cgcccacgga
    13621 cgcccggcgc gcacgtttcc ggtgcaattt cctctactac gactggacag gatctacgtc
    13681 aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc ggacatggcg acacctttct
    13741 gatcatgccc ctttaagtgc ggagattcat ttatgaaatg tagctggcgc gaaggcaata
    13801 agatccagtt gctggaaaac ggcgagcaat attatcccgc ggtgtttaag gcgattggcg
    13861 aggcacaaga acgcatcatt cttgaaacgt ttatctggtt tgaggatgac gtcggcaaac
    13921 agctgcatgc ggcactactg gcagcagcgc aacgcggggt taaagcggaa gtcttgctgg
    13981 atggctacgg ttcgccggat ctcagcgatg agtttgtcaa tgaactgacg gcagctggcg
    14041 tggtatttcg ctactacgat ccccgccctc gcctttttgg tatgcgcacc aatgtgtttc
    14101 gccggatgca tcgcaaaatt gtggtgatcg acgcgcgtat cgcctttatt ggcgggctga
    14161 attactccgc cgagcatatg tccagctacg gtccagaggc taaacaggat tacgcggtac
    14221 gccttgaagg gccgattgtc gaagatattc tccagtttga gctggaaaac ctgcctggac
    14281 agagcgcggc acgacgctgg tggcgacgtc atcacaaagc ggaagagaac cgccagccgg
    14341 gagaagcgca ggtattgctg gtctggcgcg ataacgaaga acatcgcgat gatattgaac
    14401 gtcactatct gaaaatgctc actcaggcgc ggcgagaagt cattatcgcc aacgcctact
    14461 tcttccccgg ctatcgattt ttacacgcct tgcgtaaagc ggcacggcgc ggggtgcgga
    14521 tcaaactgat cattcagggc gaaccggata tgccgattgt cagagtcggt gcgcgcttgc
    14581 tgtataacta tctggttaaa ggcggcgttc aggtatttga gtaccgccgc cgcccgctcc
    14641 acggcaaagt ggcattgatg gacgatcact gggcgacagt agggtccagt aatctcgatc
    14701 cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat ccacgatcgt cattttaacc
    14761 agacgctgcg cgataatctg aacggcatta ttgccgcaga ttgtcagcag gtcgatgaga
    14821 ccatgctgcc gaaacgcacc tggtggaacc tgaccaaaag cgtgctggcg ttccactttt
    14881 tacgccactt cccggcgctg gttggctggc ttccggcaca cacaccacgt ctggcgcagg
    14941 ttgatccgcc cgcacaaccg acaatggaaa cgcaggatcg ggtagaaact gaaaacacgg
    15001 gggtaaaacc ctgatgagta aatcacaccc gcgctggcgc ttagcaaaga agatcctcac
    15061 ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc tacgccaaaa aagtggactg
    15121 ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt gcgctgctta gtgcggtcgg
    15181 gctggtggtc gtcagctatc tgatttacgg ctgctatgac ctgctcgccc gcttttactg
    15241 cggtcacaaa ctggcgaagc gccaggtgat gctggtgtcg tttatctgct acgccttcaa
    15301 cctgacgctc agtacctggg tcggcggcat tggtatgcgc tatcgtttgt actcccgact
    15361 agggttaccg ggcagcacta ttacgcggat tttctcgctc agtattacca ccaactggct
    15421 gggctatatt ttactggcag ggattatctt taccgcaggc gtggtggagt tgccggatca
    15481 ctggtatgtc gatcaaacta cgctgcgtat tctcggcatt ggcttactga tgattatcgc
    15541 ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat atgaccatca aagggcaaaa
    15601 gctggtgctg ccttcatgga aattcgccct cgctcagatg ctgatttcca gcgttaactg
    15661 gatggtaatg ggggcgatta tctggctgtt acttggtcaa agcgtgaact atttctttgt
    15721 actgggcgtg ttactggtta gtagtattgc tggcgtcatc gtgcatattc cggtggggat
    15781 cggtgtgctg gaagcggtgt ttatcgcact actggctggg gagcatacct cgaaagggag
    15841 cattatcgcc gccctgctcg cttatcgcgt cctgtattac tttatcccgc tgctgctggc
    15901 gctgatctgt tacctgctac tggaaagcca ggcgaagaag ctacgggcga aaaatgaagc
    15961 ggcgatgtga agtttagtta agttctttag tatctgcatt tacgactaat gaaaaaaacg
    16021 cgtatgcctt tgccagacaa gcgttatagc tatcgcggca agtatcccaa agaagccgat
    16081 aaaatagtaa tagagtgtta atgcacctgt aacagcgcaa cgaaccggac tccacaactc
    16141 ttcactatgg catttccccg ccaattcaag gagcttattt cgtccatgcg agataatgcc
    16201 tgaccataac acaatcatta agcaacttat tacccaaacg ggtcgttcac tcattaatgc
    16261 ggcattcatg atgatcacca gggccaaccc tgtcaacgtc atcataatga taaaacggtg
    16321 agaaccagga tcaacattaa ataataaacc ggccagcata ctgattaaaa acatcgcgcc
    16381 cgcagtgctg aacacgacga taatggcagg tacaggagct aaacttgcaa cgacaatcgc
    16441 caccaaagag agtaaaaaca gatatattac gcctgcgatg acggcattta tcttgttgat
    16501 atccttactt aagccataca tacaaactaa taataaccct atggcgacat acattgttgg
    16561 tgaatcatat ttggttgccg taatttcgag atacttagct ccccaggcca gtcctgcggt
    16621 tgccaccaaa cctaaagcgg taatgagcca tataccgttt aatatcctgg tctcgtgctg
    16681 aaaatctaat ttattgctgt tttgtgagta tgattccatt cttttagttg tccttagaaa
    16741 aaatattaat attcaccatc aataaattta aaaccacgca tacaaaatac ttttgtattt
    16801 atggatacgg aaaacgcttc cgctcgccat aaggcgtatt ttatacttgg tacttgttaa
    16861 gtgtcgtttt tagtaagaaa aaaaatcccg acacatggcc gggatattga tggtgaaaaa
    16921 gaattaacgg cggttgccaa aaattcgcag caacatcagg aacaggttga tgaagtccag
    16981 atacaaggtt aacgcgccaa gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc
    17041 aatctgctca cccatatttt tcaatttctg agtgtcatac gccgtcaatc cgacaaagac
    17101 aatcacgccg atgtaggtaa ctgcccacat caatgcttcg cttttcagcc agaagttgac
    17161 cagcgatgcc agcacaatgc cgattaacgc cataaacagc atattgccaa agccacttaa
    17221 atcgcgcttc gtggtgtaac cgtacaggct cattgcgccg aacatcccgg cagtaacgac
    17281 gaaagtactg gcgatagaag cagcggtata gacaatgaat atactggaaa gcgtaagacc
    17341 cgtcagcgcc gaataaagca taaagagcat cgtcgttaca cctgcgctca gcttttgaat
    17401 catcgctgat aacacaataa ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac
    17461 acggttagta aacaacagct ccatcacggc cgcggaatta gccgcatacc aggcaacaaa
    17521 tgcggtcagc aacaagccaa cggtcatcca gccatagact tgcgccatat aagtttgcaa
    17581 gccagcccgg ggttgtacga ttgaatcaga acgtgggaat ctgtccatga cgatctcctg
    17641 aagatataag gaatatctta aggatactgc aaaatgatga ggctgtgcat cgacgcagcg
    17701 taaacgcatg tactgagcgg tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa
    17761 aaaactacca gcgttttgcc gcctgctgat cgctctcccg agcttcaacc cagcggtcgc
    17821 cttccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa
    17881 actgcccggc ttcaaacgca ctgctgcgat gcgcactggt gacaccgaca aaaacgattt
    17941 catcgcccgg ccataattcc ccgatgcggt gaatcacagt gacgcgcccc agcggccagc
    18001 ggttacgcgc ttcatcaaca atttctgcca gtgctttttc agtcatcccc ggatagtgtt
    18061 cgagggttaa tgctttgacg ctgtcgccca ggttatggtt gcgcacctta ccagtaaagg
    18121 tgactaccgc accgtcttcg tcacgctccg ccagccacgg gtactcttct cctacgctga
    18181 acggctgcgg accaacaaca attttggttt ctgccatctt aacctccggt taccggcggg
    18241 aagaaagcta cttcgtcgcc gtcagtcagc ggatggtcaa aactcaccag cgtctggttg
    18301 acggcagcca gtaatttgcc atcttccagc gccagcgccc agcgatcgct ctgcgcagcc
    18361 atgtgctggc gtaacgcttc aacagttggg aaatccgcag ccacttcggt tgcatctgtt
    18421 cccaccaact cgcgcacctg ggcgaaaaaa agaactttaa tcatccgctt ccaccttaaa
    18481 gtcacccgac ttgccgccgc tcttcgccag caaacgtacc ggaccaatca ccatatcttt
    18541 ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaatgc
    18601 ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac gcacccgatt
    18661 gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg gatgacagag
    18721 cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa
    18781 tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt
    18841 gacaaaggct tccgcccgcg cttcacgcac ggtttccgct ttggcggaga catccaccat
    18901 gtgcgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt
    18961 ggatggaaat tacacggacg cgtacgggca tccagctgcg gcgcgatgat attttcccat
    19021 gcggtacggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttggcgacg
    19081 cccgctaccg cacgagattg caacgtggaa gtgccaatct cttcaaacga caacatacgg
    19141 aacacttcac caaaaccttc aatttcacgg tcgaacaacg gcagcaatgc ttcgggagcc
    19201 tgatcacctt ccgtcaggcc agtaccaccc gtaatcaata ccacttgtac atcgtcgctg
    19261 gcgatccacg cagatacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta
    19321 tcgacaacgt gatggcccgc ttcttgcgcc gaatcgcgca gatagtgacc ggaggtatcg
    19381 tcttcttcac cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca
    19441 gtgcttacct gactcatctg atctctcctt ttgacgtttt agccgccaat gtacgataag
    19501 ttttgcgtaa taccggtgtt gttttgatgc aggaaatggg tctgtttctt ctcccgcagc
    19561 gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc
    19621 aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc
    19681 aggcggttgc aagtggcgca gaagtctttt tcatacggca tgataaggcc aatctctccg
    19741 gcgtaatctg gatggcaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg
    19801 atccagccgc gacgcagtag ctcgtcacgc agaacctgac cagagatgtg atgcttacgg
    19861 aagagctcgc tgccctcgcc cgtttccatc agttcgatga aacgcagctg gatagggcga
    19921 tgctggatcc agttcagaaa ggtgtcgagc tggtgatgat taacatcacg catcagcacg
    19981 gtattgactt tgaccttctc aaaaccggcc tcaaatgcag catcaatccc cgccatgacc
    20041 tggttgaatt tatcctgccc ggtaatagcg tgaaactggc gggcgtccag actgtcgaca
    20101 ctgacgttaa tgccagtaag tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg
    20161 taaccattgg tggtgaccgc aatctggcgg atagcgtcgt tttcccgcac agcggcgatg
    20221 atatcggtaa agtcgcggcg taaagacggc tcaccgcctg tcaggcgcac tttttcggtg
    20281 cccagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtaag aaagccttta
    20341 ttggtgacgc cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg aaagttacac
    20401 acatcggtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt
    20461 gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg
    20521 gcaatcttta cgagcattgt cacggccaaa gcaccgtatc agttgaccca ggtgcagagg
    20581 ctagagtgtt tagtggttat gccgatacta gcgtgtaaat gagattttta ccatccacac
    20641 tttcgctatg taatcatgta tatagcgtca tgatcgtgct atttcgctac agaataaggg
    20701 aaaatggcgg ttttactttg atatacatca tcagggaggc atagcgtgca tcgtccttta
    20761 gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttccccgca
    20821 atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc
    20881 tggctgatct tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct
    20941 catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacc gataatggtg
    21001 gctcgacggg gcgtattcgc cgttcagaag gcggcattgc ctggggcgat atgcgcaact
    21061 gcctcaacca gctgataacg gaaccgagcg tcgcctccgc gatgtttgaa taccgttttg
    21121 gtggcaatgg cgaactttcc ggtcataatc tcggaaactt gatgttaaag gcgctggatc
    21181 accttagcgt gcggcctctg gaagccatca atttaattcg taatctgctg aaagtggata
    21241 cgcatttgat tccaatgtca gagcatcctg ttgatctgat ggcgattgac gatcaggggc
    21301 atgaagttta cggcgaggtc aatatcgacc agttaactac gccgattcaa gagttattgt
    21361 taacgcctaa tgtacccgca acgcgtgagg cggttcacgc tatcaatgaa gcggatctca
    21421 tcattattgg gcctggcagt ttttatacca gcctgatgcc aattctgctg ctgaaggaaa
    21481 tcgcccaggc attacgccgc acgccagcgc cgatggttta tatcggcaat ctggggcgtg
    21541 agttgagttt acctgcggct aatttgaagc tggaaagcaa gctggcaatt atggagcagt
    21601 atgttggtaa aaaggtcatt gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg
    21661 tgaaagagcg gattgtgatc caggaggtac tggaggccag cgatattccg tatcgtcatg
    21721 accgccagtt gttacataac gcgctggaaa aggcgttaca ggctttaggt taacggaaat
    21781 ccgaaggaaa attccggctt cctattgaag acaaagtgcg cgttgtttat gccggatgcg
    21841 gcgtgaacgc cttatccggc ctacaaaccg cgcaaattca atatattgcg gagaaaatgt
    21901 aggcctgata agcgtagcgc atcaggctgt tttccgtttg tcatcagtct tcttcgctat
    21961 cctgttacga tgccgcgata aacagctcac gcagctgatg caactggtca cgaatttgcg
    22021 ccgcttcttc gaactccaga ttctgcgcgt gttgcatcat caacccttcc agctcatgga
    22081 ttttctgctg caacgcttta ggcgacatat ccatcggcac attatccggc tcaacaatcg
    22141 gtcgcgattt tcctctgccc ttcgctttgg ttttagcaat gttctgcccc aacgccagga
    22201 tatcgaccac tttcttgttc aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct
    22261 gctgtttctc gcggcgacgt tcggtttcgc caatcgcttt cgccattgat ggggtgatct
    22321 tatcgccgta gagaatcgct ttaccgttaa cgttacgtgc cgcacgacca atggtctgga
    22381 tcaacgaacg ttcggaacgc aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg
    22441 aaacttccgg catatccaga ccttcacgca gtaagttgat ccctaccaat acgtcgaact
    22501 cacccagacg caagtcgcgg ataatctcca tacgctcgac ggtgtcgata tctgagtgaa
    22561 gataacgcac gcgctcaccg tgttcttcga gatattcggt aagatcttcc gccattcgct
    22621 tggtcagtgt ggtgaccagt acgcgttcgt taattgccgc tcgctgacga atctccgaaa
    22681 gaagatcatc aacctgtgtc gccaccggcc gcacttcgat aatcgggtca agcaatccgg
    22741 ttggacgcac cacctgatcc accacatcgc cgccggattt ttccagctcg taattacccg
    22801 gcgtcgccga aacatagatg gtttgcggcg ctaatgcttc gaactcttca aacttaagcg
    22861 gacggttatc cagcgctgat ggcaggcgga agccgtactc caccagtgtc tctttacgcg
    22921 cccggtcacc gcgatacatg ccgccaattt gtggaatggt gacgtgagat tcatcgacca
    22981 ccagcagccc atcagcaggg aggtaatcaa acagcgtcgg cggtggctca cccggtccac
    23041 gaccggagag gaagcgcgag tagttttcaa tccccgaaca gtagcccagc tcgttcatca
    23101 tctccagatc aaactgggta cgctgggtca gccgctgctc ttccagcagt ttgttgtttt
    23161 ccaacagcac tttgcgtctg gcggccagct cttctttgat ctcctccatc gcctgtacga
    23221 tgcgctcgcg cggtgtgacg tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg
    23281 aaacaatctg cccggtcagc gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca
    23341 gttccacgcg aagtgcaatg tcatccgatt ctgccgggaa gatatctatc acctcgccac
    23401 gaacgcggaa agtaccacgc tggaatgctt gatcattacg agcgtattgc agctccgcca
    23461 gtcggcgcag aatcgcgcgc tgatcgataa tcataccgac cgtgagatgg agcatcatct
    23521 tgagatataa atcaggatcg cccagaccat aaatcgcgga aacagacgcc accacaacca
    23581 catcacgccg ctccagcatc gctttggtgg cggacaaacg catctgctca atatgttcgt
    23641 taaccgaggc atctttctca atgaaagtgt cggaactcgg tacataggct tccggctgat
    23701 agtagtcgta gtaggaaacg aaatattcca ccgcgttttc cgggaagaac tctttcattt
    23761 cgccatacag ctgggccgcc agcgttttgt tgggcgcaag taccatggtt gggcgctgaa
    23821 ggtcagcaat gacattggca atggtgaatg ttttcccgga gccagtcacg ccaagtaacg
    23881 tctggtgcgc caggccatct tccagcccct cttcgagacg tcgaatcgcc tctggctgat
    23941 cgccagaagg tttaaaagcg gaattcagtt tgaacggttt actcatgagt cgctacctga
    24001 aggagttggg cgggcaggta tgtaatttta ctcgtcgtac ttaattatgc caacaaatta
    24061 tactggataa aaaaacagtt catcaccata atatttctgt tacagcgtaa actccgctgt
    24121 caatcatgag caaaatttac tctgtggcga gataaaactc cggccttacc gggttatccc
    24181 caaagcaacg gcttttttaa catttgtcaa gatgagtgat gacagtttta tggcaagaga
    24241 tgcctgttca gtgactgcca ttgcatttat ctaaccagtt aaaaaataaa agatatttct
    24301 ttgagccgtc tttaacgcca attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt
    24361 tttctaactc taatacacat ggttatccac aggaatagtg gataactgtc tccagcccct
    24421 atcccccgcc gcctgggcaa ttcccacatt cgccgcatga ggcggaagta aaattttttt
    24481 cgtgattatt ttagaattta attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc
    24541 gcaatttaac caaatacagg atagctacaa caaggcaagg tttatgtact ttccggttgc
    24601 cgcattttct ggattttctg caagccaggg gatctctccc agcagcggcg cgggaatcat
    24661 gcgggtgagc gtggtcatat attcagcgtg acgttttccc ggaggcgtaa catcgttcgc
    24721 cacccaaccc gccagagtca gtccggcgtg ttgtattgcc tgcgcagtca acatcgcgtg
    24781 attaatacag ccgagtttca caccaactac cagtatcacc ggcagttgtt cctgtgttac
    24841 ccaatctgca aaagtgaaag tgtcagaaag cggcgtaaac cagccgccag caccttccac
    24901 taacacccag tcagcctgtt gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc
    24961 tatcggcctg ccctcttgcg cgctgatgat gtgcggcgag gtgggttctg cgaaggtgta
    25021 aggatttacc gttgcgtaat ccagctgcag gctgctgttg tgctgtaacg ccagcgcgtc
    25081 actattacgt aagccttccg gggtcttttc gctgccagag gcgaccggtt tataacctgc
    25141 cgtccgataa cctactgcct tcgcggcttg taaaagtgca caactggcga cggttttccc
    25201 cacttcggta tccgttccgg tgacaaaata acgtttactc acgagcaatc actcccaaaa
    25261 aaagatgata cgtcagagga tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct
    25321 gcaattgcga acgcgttaat attcgcgggt cgcgcccttc atgaagatgc gtagcaccga
    25381 tgcctttcag cgaacgcatg gcactgagcg catcatcaaa ccatagtgtg atgggctgaa
    25441 tatgatgttg ataatgcacg ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc
    25501 gattagcatg cggacgctcg tccaccgcct gccacgcctg atgcagttcg ggtaacgatc
    25561 cctgcaccag cgtggtaaac gccaccacgc ctccggggcg caccacccga tacagctcgc
    25621 ggagtgccgt ggataaatta ccgcaccact gcactgcgag attgctccat gcaagatcga
    25681 acgtcgcagt cgctaacggc agggattcga tatctcccgc cagataatgg tctgcggcat
    25741 ccttctggcg tgcctgaaca agcattggcg gcgagagatc taaggccgtc acctgcgcgt
    25801 gacgttcccg ccagtggcgg ctcatccagc caggtccaca acccgcgtcc agtacgtggg
    25861 tgtatttacg ctgtgggagc attgccagta agacgtcagc actctggcgc tgtagatctg
    25921 cgtgttgctc atagagtgcg gctgcccgac caaatgccgc tgcaatggct tgtttattaa
    25981 ccgttgccat gcagcacctc cagcagacgg tcgatatcct gcatttcatg cgctgcggtt
    26041 agcgttaagc gcagtcgcgc agtaccagcg ggtacggttg gcgggcgaat cgccgtgacc
    26101 cagcagcctt gctggcgcag tttttctgcc agttgtaacg cacggctgtt atcaccgacg
    26161 atcaatggct ggatggcgct gcatgaatca gccagcgtaa acggcaaatc ctgtactccg
    26221 gcacgaaaac gcgcaatgag tgacaccagt ttttcgcgcc gtgcatcccc ctcatcacgg
    26281 cgaatgaccg ccagtgacgc acgtaatgcc tgcgcctgag cgggcggcat actggtgctg
    26341 tagataaggt ggcgggcgaa ttgcagcaga taatccgcca ccgtactgga gcaaagcacc
    26401 gcagccccac tgacgccaaa tcctttgcca aaagtcacca ccagcaattc tggttttacc
    26461 ttttgcagcc agcagctgcc gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca
    26521 tcgaccatca accagccatt gtgctgttgc gttacctgct ggatttccgc cagtggcgca
    26581 atatcgccgt ccatgctgaa cacgccttct gtcaccacca gttgctgccc cggacagggg
    26641 gaagcaagca gtcgcgccag atgagtgaca tcgttatgaa caaaacggcg aagctgcgac
    26701 gggcttaaac tggcagcctc cagcaatgag gcatggctaa gccggtcagc gacaatgcgg
    26761 tcctcttttg ccatcatcgc ggcaatgacc gcctgattag cggcaaaacc agagataaat
    26821 agcagtgccc gcgaatagcc cagccactcg gccagttctt cttccagcgc ctgatgcgca
    26881 acgctataac cgctgacgtg accggaaccg ccgctaccga cgccaaattg ctccgcaccc
    26941 tgctgccagg cacggataat ttgcggatga tggcttaaac ctaaataatc gttactggaa
    27001 aagttcagat actggcgatc atccgccacc agccagcgtc cggctccttg cgccactgga
    27061 taacggcgac gcagggcatc ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc
    27121 tgccagctca taatgctgcc gcgttgtaat attcgtcggt gtccggggtc atcagcgcct
    27181 gttcaagacg ttgctgttgt tcgttatccc ctgccagcac ggcagtttgc tgcggattta
    27241 gccccagttt gcggaacagt tgcaggtctt tatcttcttc cggattcggc gtggtcagca
    27301 gtttgcaacc gtagaaaatc gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt
    27361 cgttcatctg ctcgcgtccg gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg
    27421 cgaccgcaat ggtgcgaata aaatcaaagg catcgacatc atcgttatcc gccagcggcg
    27481 tgcctttcac cttcaccagc atgttgattg gtacgctttc cggcggcgtc ggcaggtttg
    27541 ccagttgcag caataatccg gcgcgatctt ttaccgtttc gcctaagccc acaatgccgc
    27601 cagaacagac tttgatcccg gcatcgcgca ctttttccag cgtatcgagg cgttcctgat
    27661 aagtgcgggt ggtgatgata ttgccgtaaa actccggcga ggtgtcgagg ttgtggttgt
    27721 agtaatccag cccggcattc gcaaggcgct gcgcctgaga ttcactcaac gtgcccagcg
    27781 tcatacacgc ctccagcccc atcgctttta ccccctgcac catttgttcc aggtacggca
    27841 tatcgcgttc gtggggattc ttccacgccg cgcccataca gaaacgcgtc gatcccgcag
    27901 ctttcgcttt gcgcgccgac tccagcacct gttcaacttc catcaaccgc tcggcttcca
    27961 gcccggtttt gtagcgcgag ctttgcgggc agtatttgca atcttccgga caagctccgg
    28021 tcttaatcga cagcaaagtg ctgacctgca cctgacgagg atcgaaatgc tgtcgatgca
    28081 cctgctgcgc ttcaaacagc agatccagca acggtttttc aaataattct gtgacttgcg
    28141 acaatgtcca gcgtgggcgg tgagccatgg ggcttctcca aaacgtgttt tttgttgtta
    28201 attcggtgta gaattgtaaa cctaaatctt ttcaatttgg tttacaagtc gattatgaca
    28261 acggacgatc ttgcctttga ccaacgccat atctggcacc catacacatc catgacctcc
    28321 cctctgccgg tttatccggt cgcgagcgcc gaaggttgcg agctaatttt gtctgacggc
    28381 agacgcctgg ttgacggtat gtcgtcctgg tgggcggcga tccacggcta taatcacccg
    28441 cagcttaatg cggcgatgaa gtcgcaaatt gatgctatgt cgcatgtgat gtttggcggt
    28501 atcacccatg cgcccgccat cgagctatgc cgcaaactgg tggcaatgac gccgcaaccg
    28561 ctggaatgcg tttttctcgc ggactccggc tccgtagcgg tggaagtggc gatgaaaatg
    28621 gcgttgcagt actggcaggc caaaggcgaa gcgcgccagc gttttctgac cttccgcaat
    28681 ggttatcatg gcgatacctt tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac
    28741 agcctgtgga aaggctatct gccagaaaac ttgtttgctc ccgccccgca aagccgcatg
    28801 gatggtgaat gggatgaacg tgatatggtt ggctttgccc gcctgatggc ggcgcatcgt
    28861 catgaaatcg cggcggtgat cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg
    28921 taccatccgg aatggttaaa acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg
    28981 attgccgacg agatcgccac cggatttggt cgtaccggca aactgtttgc ctgtgaacat
    29041 gcagaaatcg cgccggacat tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc
    29101 ctttccgcca cgctcaccac gcgcgaggtt gcggaaacca tcagtaacgg cgaagccggt
    29161 tgctttatgc atgggccaac ttttatgggc aatccgctgg cctgcgcggc agcaaacgcc
    29221 agcctggcga ttctcgaatc tggcgactgg cagcaacagg tggcggctat tgaagtacag
    29281 ctgcgcgagc aacttgcccc cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg
    29341 ggggcaatcg gcgtagtcga aaccactcat ccggtgaata tggcggcgct gcaaaaattc
    29401 tttgtcgaac agggtgtctg gatccggcct tttggcaaac tgatttacct gatgccgccc
    29461 tatattattc tcccgcaaca gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag
    29521 gatgaaacat ttttttgcca ataacgagaa gtccgcgtga gggtttctgg ctacactttc
    29581 tgcaaacaag aaaggagggt tcatgaaact catcagtaac gatctgcgcg atggcgataa
    29641 attgccgcat cgtcatgtct ttaacggcat gggttacgat ggcgataata tttcaccgca
    29701 tctggcgtgg gatgatgttc ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc
    29761 ggatgcgcca accggctccg gctggtggca ctgggtagtt gttaacttac ccgctgatac
    29821 ccgcgtatta ccgcaagggt ttggctctgg tctggtagca atgccagacg gcgttttgca
    29881 gacgcgtacc gactttggta aaaccgggta cgatggcgca gcaccgccga aaggcgaaac
    29941 tcatcgctac atttttaccg ttcacgcgct ggatatagaa cgtattgatg tcgatgaagg
    30001 tgccagcggc gcgatggtcg ggtttaacgt tcatttccac tctctggcaa gcgcctcgat
    30061 tactgcgatg tttagttaat cactctgcca gatggcgcaa tgccatctgg tatcacttaa
    30121 aggtattaaa aacaactttt tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa
    30181 aagctgaacg cgaaacgcta aagaccatta atatcaatgt gttacaatat atttagtcta
    30241 aaaaatagac tgcataatac tacaaaacac aacatatcta gtaactatga atcaactact
    30301 tagatagtat tagtgacctg agacagagca ttagcgcaag gtgatttt
//
LOCUS       DAZFKM010000053        30237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFKM010000053 DAZFKM010000000
VERSION     DAZFKM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..1726
                     /gene="yegH"
                     /locus_tag="ACHKAY_002419"
     CDS             143..1726
                     /gene="yegH"
                     /locus_tag="ACHKAY_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO5701087.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(2000..3853)
                     /gene="asmA"
                     /locus_tag="ACHKAY_002420"
     CDS             complement(2000..3853)
                     /gene="asmA"
                     /locus_tag="ACHKAY_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO5701088.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3875..4456)
                     /gene="dcd"
                     /locus_tag="ACHKAY_002421"
     CDS             complement(3875..4456)
                     /gene="dcd"
                     /locus_tag="ACHKAY_002421"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO5701089.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4548..5189)
                     /gene="udk"
                     /locus_tag="ACHKAY_002422"
     CDS             complement(4548..5189)
                     /gene="udk"
                     /locus_tag="ACHKAY_002422"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO5701090.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5507..8817
                     /gene="dgcE"
                     /locus_tag="ACHKAY_002423"
                     /pseudo
     CDS             5507..8817
                     /gene="dgcE"
                     /locus_tag="ACHKAY_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(8856..9713)
                     /gene="alkA"
                     /locus_tag="ACHKAY_002424"
     CDS             complement(8856..9713)
                     /gene="alkA"
                     /locus_tag="ACHKAY_002424"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO5701091.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9846..11198
                     /gene="yegD"
                     /locus_tag="ACHKAY_002425"
     CDS             9846..11198
                     /gene="yegD"
                     /locus_tag="ACHKAY_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5701092.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11231..13150)
                     /gene="yegI"
                     /locus_tag="ACHKAY_002426"
     CDS             complement(11231..13150)
                     /gene="yegI"
                     /locus_tag="ACHKAY_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO5701093.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(13147..13908)
                     /gene="pphC"
                     /locus_tag="ACHKAY_002427"
     CDS             complement(13147..13908)
                     /gene="pphC"
                     /locus_tag="ACHKAY_002427"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO5701094.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13905..14564)
                     /gene="yegL"
                     /locus_tag="ACHKAY_002428"
     CDS             complement(13905..14564)
                     /gene="yegL"
                     /locus_tag="ACHKAY_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5701095.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14785..14844)
                     /gene="ibsA"
                     /locus_tag="ACHKAY_002429"
     CDS             complement(14785..14844)
                     /gene="ibsA"
                     /locus_tag="ACHKAY_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO5701096.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15117..15173)
                     /locus_tag="ACHKAY_002430"
     CDS             complement(15117..15173)
                     /locus_tag="ACHKAY_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5701097.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15445..15501)
                     /locus_tag="ACHKAY_002431"
     CDS             complement(15445..15501)
                     /locus_tag="ACHKAY_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO5701098.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15780..17027
                     /gene="mdtA"
                     /locus_tag="ACHKAY_002432"
     CDS             15780..17027
                     /gene="mdtA"
                     /locus_tag="ACHKAY_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO5701099.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17027..20149
                     /gene="mdtB"
                     /locus_tag="ACHKAY_002433"
     CDS             17027..20149
                     /gene="mdtB"
                     /locus_tag="ACHKAY_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO5701100.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20150..23227
                     /gene="mdtC"
                     /locus_tag="ACHKAY_002434"
     CDS             20150..23227
                     /gene="mdtC"
                     /locus_tag="ACHKAY_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO5701101.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23228..24643
                     /gene="mdtD"
                     /locus_tag="ACHKAY_002435"
     CDS             23228..24643
                     /gene="mdtD"
                     /locus_tag="ACHKAY_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5701102.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24640..26043
                     /gene="baeS"
                     /locus_tag="ACHKAY_002436"
     CDS             24640..26043
                     /gene="baeS"
                     /locus_tag="ACHKAY_002436"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO5701103.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26040..26762
                     /gene="baeR"
                     /locus_tag="ACHKAY_002437"
     CDS             26040..26762
                     /gene="baeR"
                     /locus_tag="ACHKAY_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO5701104.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26953..27285
                     /gene="yegP"
                     /locus_tag="ACHKAY_002438"
     CDS             26953..27285
                     /gene="yegP"
                     /locus_tag="ACHKAY_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO5701105.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27494..27790
                     /locus_tag="ACHKAY_002439"
     CDS             27494..27790
                     /locus_tag="ACHKAY_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5701106.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27792..28088
                     /locus_tag="ACHKAY_002440"
     CDS             27792..28088
                     /locus_tag="ACHKAY_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5701107.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28191..29552
                     /gene="yegQ"
                     /locus_tag="ACHKAY_002441"
     CDS             28191..29552
                     /gene="yegQ"
                     /locus_tag="ACHKAY_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO5701108.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29882..30199)
                     /gene="yegR"
                     /locus_tag="ACHKAY_002442"
     CDS             complement(29882..30199)
                     /gene="yegR"
                     /locus_tag="ACHKAY_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO5701109.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6992 a         8063 c         7978 g         7204 t
ORIGIN      
        1 cacttttaat tcagggataa tgtgaggtta ttaccctcaa ataatatgag ataaatagtg
       61 ctgcaacatt gcatttttgc cccgatttat ccatgatcga attatgacat ttgtcataca
      121 acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct gggccgggtt
      181 aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct ttattgccat
      241 cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg ggctgctgct
      301 ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca ccctgactca
      361 accgctgttc agcttctgct cgtttacctt tagcgcccgc gacttaatca tgctgtttgg
      421 tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg aagggaaaga
      481 cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggcgtggtga cgcaaattgt
      541 ggtactggac gccatcttct cactcgactc agtgattacc gccgtcggga tggtcgacca
      601 tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga tggccagcaa
      661 gccgttaacg caatttgtta acagtcaccc gaccatcgtt attctctgct taagcttcct
      721 gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc cgaaaggcta
      781 cctgtacgct gccattggtt tctcggtgat gatcgaggcg ctcaatcagt tggctatctt
      841 taaccggcga cgttttcttt ccgctaacca gacgctgcgc cagcggacta ccgaagcggt
      901 aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa ccgcgtccat
      961 gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga tgattgagcg
     1021 ggtacttaat cttaaccagc gtactgtcag cagcattatg acgtcgcgcc acgatattga
     1081 gcatatcgat ctcaacgcgc cggaagaaga aatccgccag ttgctggagc gaaatcagca
     1141 tacgcggctg gtggttaccg acggtgatga cgcagaagat ttgctcggtg ttgttcacgt
     1201 tatcgacctg ctacaacagt cactgcgcgg cgaaccgctc aacctgcggg tgttgattcg
     1261 ccagccgctg gtgttcccgg aaaccttgcc gttgttacct gccctggagc agttccgtaa
     1321 tgcccgcacg cactttgctt ttgtagtgga tgagtttggc tcggtggaag ggattgtgac
     1381 attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg aagagatcga
     1441 cgcccgccat ggtattcaga agaatgcgga cggttcctgg acggcgaatg gtcatatgcc
     1501 gctggaagat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg aatatcacac
     1561 cattgccggg ctgttgatgg aatatttaca gcgtattcca aaacctggcg aagaagttca
     1621 ggtgggggat tatttgctta aaacgttgca ggtggaaagc catcgcgtgc agaaggtgca
     1681 gattattccg ctgcataagg atggcgagtt ggagtacgag gtgtgatggt atcggtcatt
     1741 ttacccgata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat ttaccgccgg
     1801 gagcccgggt tgccagggtg gtggcgattg agccaccctg gcacgttcac cggcttgagc
     1861 gttccatagt agtgaggaac atcaagtgaa cggaacagcc ttcgatctta catattcaga
     1921 atataatggt gccgccgcga acgcctgatg cgacgcttta cgcgtcttat caggcctaca
     1981 ggacaggtca cctggtaacc tacatcttct ccagcaactt cttcacatct ttgccattgc
     2041 gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg tcctgtagat
     2101 gtttgcgcag taactgatcc acttgcagac tgtaattgag ttgctgccag ttgccataaa
     2161 cccgcagcgg tactggcgtt tctttgagga aatcaatcag tttgctttcc ccgttccagc
     2221 cacccacgac ccgaatatca aactgggtgt cgcaggtttg atctgccaga ttcaacatgc
     2281 cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac gtcacgacgc
     2341 catccttcaa cgtcaaatcg gtggtaaagc ggtcaagacg cgttacgtta tcgaagtttt
     2401 cagcggcctt cacatcacca ccattacgtt ctaccgcttg ctgaatcatc tgctggaagt
     2461 tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc cagttgtggc
     2521 ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac atttttccgg
     2581 tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg ttttccagcc
     2641 gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc ggcagtgaaa
     2701 cctgtccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc gacttgttgg
     2761 tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta ctggcctgca
     2821 acaaaatatc agccgtaaag ccttgcagtc cctgatatgc cggttcatca atacgcgaag
     2881 aaatgaccgg gcgcggcaat gttgattggc tctgcccctg ctgcgcggca ccgttttcac
     2941 cattcgctgt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg aattgcagtc
     3001 tcagctgcca ttcaggtttc tctgtcagcg tgacctgtgc ttgcccgctc agcgtgctgt
     3061 cattggcggt caagctgatt tggttaaatg aaaggcgttt atgtgactcc tgccactggg
     3121 cctggaaact cccctgcccc tgaatacctt gttttggtaa atctgcaccc tgcaactgcc
     3181 agttaatttg ttcaatagcc gccgttaaat catgcggata atcagaagca tctaccgtac
     3241 cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag aactcaaatg
     3301 agccacgatg ttggggatct tgttccattt gcaggcggat attgcggatt gtcacctgct
     3361 cgtcatcttc atgctggaac accagcacgc tgtccgccac cttaagactg gatatatcaa
     3421 acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt gcaaccggag
     3481 cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc ccttttagca
     3541 tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg tccagacgca
     3601 tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc ggtgagagac attcgcccgg
     3661 agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc tgcaattgat
     3721 aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc ggattcacca
     3781 gcaacactaa cgcagataac ccggccacca gcacgaccag gagtatcatc agcgtcgtca
     3841 gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt cggctggcta
     3901 ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt cgcgccgccg
     3961 ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc agcgccagcg
     4021 gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc ggatcgatac
     4081 ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc ccgtccagcc
     4141 agcccaccag atcggctggc agcgtcaccg actccagcgt caccgccagc gccagctctc
     4201 ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc atcacgcggt
     4261 caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct gccgtgtgac
     4321 cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg ttaatacgct
     4381 ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct tcaatatctc
     4441 ggtcacacag acgcatggca ttttctcctt tcgcgcatca ctcccttaac gccaattacg
     4501 tcaagggcat accaggttat cactgaacgg tacacaattt atcaagctta ttcaaagaac
     4561 tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc gcgcggcacg
     4621 ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg gcgcacggtt
     4681 ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac gtcacgcttg
     4741 atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa gttaagttcg
     4801 tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac cttcttcggc
     4861 tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac cggcaggtca
     4921 attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca acaggctgtg atccatcgcg
     4981 ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct ttgatctttg
     5041 taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc acgtaattca
     5101 cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc gataccgata
     5161 atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac ctggatgtta
     5221 ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg cctgatacca
     5281 gtcgaatagc gctgccgcgc gctcagagtt aattgttgac gaagaattcc cggtggcaaa
     5341 ttacgttgat cagttttata caaggtaaaa aatgttatac gcagttgcgc aaattatccg
     5401 cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt actagcataa
     5461 acacagaaac gaatactggc gacctggtct tgcggataaa gcggtaatga gcaaacaatc
     5521 acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt taggtttagt
     5581 ctcgtttatc tttacccttt tctcgcttga gctttcgcag tttggcaccc aactcgcccc
     5641 actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg ggcgcatgtg
     5701 gccgggaatt gcgctgagct gctcgctggg aaatatcgcc gcatccatcc tgcttttttc
     5761 caccagctcg ctgaacatga cctggacgac catcaatatt gttgaagccg tggtcggggc
     5821 agtgctgcta cgtaaattgc tgccgtggta taaccccttg caaaatctgg ctgactggct
     5881 gcgtctggca ttcggcagcg ccattgttcc gcctctgcta gggggtgttc tggttgtcct
     5941 gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt cagaatccat
     6001 cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc tgctgcgcca
     6061 tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac tgacgttaag
     6121 ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc tgttgatgtg
     6181 gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca cggtgatgat
     6241 ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta catacctgat
     6301 gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca tcatgacgat
     6361 ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa cccgttttcg
     6421 gaacgccatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg gacaatggct
     6481 gcaaaccaac aaagcgctct gccagtttct cgggtacagt caggaagagc tgcgcggact
     6541 cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac aggttgaaaa
     6601 gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca accgcaatgg
     6661 cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg gcacgccgct
     6721 ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac aggtgaatca
     6781 gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca tctgggagtg
     6841 ggagttgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt atgaaattcc
     6901 tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg aagatcgcca
     6961 gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaaactgga atttcgcatt
     7021 accgtaaaag acggtattcg ccatatccgc gccctcgcca accgggtact gaataaagaa
     7081 ggcgaagtcg aacgcctgct cggcattaat atggatatga ctgaggttaa acagcttaac
     7141 gaggcattgt ttcaggaaaa agagcgcctg cacataacgc tggactccat cggcgaagcc
     7201 gtggtctgta ttgatatggc aatgaaaatt acctttatga atccagtggc ggagaagatg
     7261 agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt gcatattacc
     7321 tttggcgaca atggtcccct gatggagaac atttacagtg ccgacacctc acgttccgcg
     7381 attgaacaag atgtggtgtt gcactgccgg agcggcggca gctacgacgt gcattacagt
     7441 attacgccgt taagtactct ggacggcagc aatattggtt cggttctggt gattcaggac
     7501 gttaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca tgatgcactg
     7561 acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatgctgct gcaaacggta
     7621 aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt taaagcggtg
     7681 aatgacagcg ccgggcatgc ggcgggcgac gctttgctgc gcgaactggc gtcgttgatg
     7741 ctgagtatgc tgcgttccag cgacgtgctg gcgcgactcg gtggggatga atttggtctg
     7801 ctgctaccag actgtaatgt tgaaagcgcg cgttttatcg ctacacgcat tatcagtgcc
     7861 gtgaatgact atcactttat atgggaaggc cgtgtgcatc gggtaggtgc cagtgccggg
     7921 attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca ggctgatatc
     7981 gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga accacagcaa
     8041 gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg gatgattaaa
     8101 gagaatcagt tgatgatgat ggcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt
     8161 aatttgtggc tgatttcgct taaactctgg agttgcgaag gcgagattat tgatgaacaa
     8221 aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg acgggtattc
     8281 cacgaatttt tccagctggc cgcaaaagcg gttgccagta aaggcataag catcgccctc
     8341 cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct tgagcagctg
     8401 gaaaatagcc ctctaccacc acggttatta catctgatta ttccggcaga cgcgatttta
     8461 gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt gctcagccag
     8521 gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga ttacctgcta
     8581 cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat gctgattacg
     8641 attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc agtcgtttta
     8701 cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt
     8761 gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat taactgaggg
     8821 agcgtttgcc cattgcctga tgcgacgcat caggcctaca attcgtccgt tccgtctggt
     8881 tgccagcctt ccgtatacca gatatgcaac agcgcataag aacgccaggg cttccagcgc
     8941 tcggcataac ggcggatttg tgccggtgtc atccccggaa atcgctgttt aatcagataa
     9001 tcatccggca gaaaaacatc tttcgcctgc cagccacgca aagcgaaata attcgccgtc
     9061 cagcgcccga tacccggaaa agtttgcagc gttttcatcg cctgctccac atcgcccgga
     9121 attgtcattg gtaagctgcc ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc
     9181 cgtttcaacg gcatgcctaa cgcttttaat gcctgcgggt cggctgctgc cagccgctga
     9241 ggcgtgggga agcagatata ctccggaaaa tcatccagcc gttcgccata aagctgtaca
     9301 actttggcag tcaattttgc cgccatcgcc acgctcacca gttggcctaa aatcgcccgc
     9361 acaccctgct caaaagcatc aatacagccg ggtaaacgca atccgggccg cgccgcgcct
     9421 aacttgccca acgcaccgtt aacaatctgc gggttacatt gcagatcaaa caggcggctc
     9481 attttcgcca gacactcagc ggcaacgggt tctaaacctg cacttaaatt tatgtgcaga
     9541 gtatggcggg ctatatccgg aatagcagtc accacgccgc gatattcgcc caccgccaga
     9601 ctacgggcat aataatcctc agcgaccgtt tccacaccac tcactgcacg ggcggcgaga
     9661 aatcccaaca tccacgacca gtcatacggc ggctgccagt tcagggtata catcgcatct
     9721 ccttattcat ccgctttcag cataaacgtt attcagacgc tacgctttgc tttcatattc
     9781 cggttgtcgc gacagcaaca tttcgctaaa gtcacgccct tcttcaccgg catggggatt
     9841 aattcgtgtt tattggtttt gattacggta cagcaaactg ttcagtggcg gtcatgcgtg
     9901 acggtaaacc gcaattactg aaaatggaaa acgacagcac gctgctgcct tcaatgcttt
     9961 gcgcgccaac gcgtgaatcg gtaagcgaat ggctgtaccg ccatcatgat gttccggcag
    10021 acgacgatga aacgcaggcg ctgttacgtc gggcgattcg ttataaccgc gaagaagata
    10081 tcgatgttac ggcgaaaagc gtgcagttcg gtctttcctc actggcacag tacattgatg
    10141 atccagaaga agtgtggttt gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa
    10201 aaccgcagca ggtagcgctg tttgaggatc tggtctgcgc aatgatgttg cacattcgcc
    10261 agcaggcgca ggcgcagctg ccagaagcga ttactcaggc ggtgattggt cgcccgatca
    10321 acttccaggg gctgggcggc gatgaagcaa acgcccaggc gcaagggatt ctggaacgcg
    10381 cggcgaagcg tgccggattc agggacgtgg tattccagta cgagccggtc gcggctgggc
    10441 tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat atcggcggtg
    10501 gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg
    10561 aagccagcct gctgggtcac agtggttgcc gtattggcgg taacgatctg gacatcgcac
    10621 tggcgtttaa aaacctgatg ccgttactgg gcatgggtgg cgaaaccgaa aaaggcatcg
    10681 ccctgccgat tctgccgtgg tggaatgcgg ttgccatcaa cgacgtacct gcgcagagtg
    10741 atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac
    10801 cggaaaaagt ggccctgcta cagaaagtct ggcgtgagcg tttaagctat cgcctggtgc
    10861 gtagcgcaga agagtgcaaa attgctcttt caagcgtagc ggaaacccgc gcctcactgc
    10921 cgtttatcag cgatgaactg gctacgctga ttagccagca agggctggaa agcgccctca
    10981 gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac gcccaggaaa
    11041 aaccggacgt tatctacctg accggcggta gcgcccgttc gccgctgatt aaaaaagcgc
    11101 tggcagaaca gttgccggga attccgattg caggcggcga tgactttggc tccgtcaccg
    11161 ccgggctggc acgctgggcg gaagtggtgt ttcgttaaaa gaaaatggct cagcagcggc
    11221 ttaaatctgc ctacgcctga gccagttttt ctgccgcctg acgtaaaggt tccatcaacg
    11281 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca
    11341 acgtcgattc cagccgatgt cgtttggcgg tcattttcgc cattaccgct tgtctgtctg
    11401 ctggcgttat cgcttcattc ggcctgaaca caaaacggcg ttcacagctc gctttccagt
    11461 cgataaccgc ctgggtcaga tggtcgccaa accctttcac ttgcttaacg ccacgacggg
    11521 taacatctgc ggctgtttca ataccaaaag accgtaacgc cgctttacgc gcagggccaa
    11581 cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc ttctgccgtt
    11641 cccttgcagt gtcatgaagt gctgccagag cgcgtttctc ttcttccggt aacccgagaa
    11701 tttcgtcctt cattttttcg agcatcgccc gtttggcgat aaaaccttcc agcccgccca
    11761 actgttggat ctggctgact aaatgctcat agtccatttt cgcgcgatta aatgcctcac
    11821 ggcgttgctg gatttctgct ttgtacgctt tgcttgtcag actgccaata atccagatag
    11881 ccgacagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcaaca
    11941 gcgataacgc tgacagtgcg atctcaatca gaatgatgta ttcgcgccgt aacaggccta
    12001 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcaattgc aatgctggcg
    12061 gtgctactga cgccatcacc atcgcccaga ctttcgccag cacaaaatca ccgccggtgg
    12121 taatgacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgcacacc
    12181 acgggcagtc ggtcaaatga gcgggataaa catgcattgc cgaaacggga catttcttta
    12241 actgttggcg cagagaatca agtgccgcta cccacgcttt agccgtcgga cgcccggttg
    12301 ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcgccc ggtaacatcg
    12361 atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
    12421 cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
    12481 caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
    12541 aattatcgtg attttctgtg cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg
    12601 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
    12661 gaaaggagtc gctatcaatc agtaccactt tgctgtcgcg acctaccata aaactgttct
    12721 ggtttacgtc acccacgacg tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
    12781 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga cgacgatgtg
    12841 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
    12901 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
    12961 attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
    13021 gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa accgcgcctt
    13081 caccgccttt gcccagttca cggcctggcg taacgcactc accagtagca gtatataaag
    13141 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg tacgctcgtt
    13201 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gttgttcctg
    13261 cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggtacatgag gagaattatc
    13321 cagcatattt aacgccagtc gctggatacc atccgtaaat gctgcaactt tatgcacacg
    13381 ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa tgaagtgcgt
    13441 catattggca tattcaccca ccatcggcgt aagcggcaat tgcagaccgt gaccaaaatc
    13501 caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccattagccg atgaaatcaa
    13561 tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg cctcggcaaa
    13621 aagtcgctga cgaacggtca gcacaatatc cgtggcgagg atatcattaa gccccaattc
    13681 cccaccctgc actttttgcg acatataagc catcgcttca ttgacggcga gcgtcgcgcc
    13741 ttctcctccc tgcgaaacgc tacctgcgcc atcagcaagg aacatcacca gtaatggctg
    13801 ctggtcattt agccaggcaa cctgcatctg gcaggcatcc tggcaaggca agtccgcgct
    13861 gatgtgcgat gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
    13921 ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc
    13981 gaactggata accagctaaa cagttcacgg aattgtaatc cttgcagagg caaaggctga
    14041 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaaa
    14101 gcaaaccttt tatcttcttc cccccgaaac actttgttgg ctgcagcctg ccactcatcg
    14161 gttggtgcac catcggtaat caggaaaatc cacgggcgat aatacgaaat accatttgca
    14221 cgatactcac gtttccgctc ctctaccata tccagggctt tggtaatggc cgcacccatt
    14281 ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt
    14341 tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc
    14401 ggatcggcaa gcagttcatc acgaaaggtc accaatccgg cgttaagttc attaataggt
    14461 ctgccgctca tcgagccaga aacatccagc agcaaaatgc aggggcaacg tggttccggg
    14521 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta
    14581 gagaattatc gaggtccggt atcataccga ctgcaataat gaaataagag caaacgaaga
    14641 aactttcaga attaggcccg gcggcgcaga aaattttgct attcgcctcg caagaacgga
    14701 gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga
    14761 ggtttccccc tccccctggt gttcttagta agcctggaag ctaatcacta agagtatcac
    14821 cagtatgatg acgtgcttca tcataaccct ttccttatta aaagccctct tctccgggag
    14881 aggctttccc gtttcagcgt cccgctgaaa tcatcggctt acctcctttc gccctgaatg
    14941 cagtctatcg ctaacgcgtg aataaaatag tttcctctgt gattattgcg taacgactcg
    15001 caaagaatat ttgctggcag gatcgcagac tacaaagcct gcggattgac aatcttatcg
    15061 tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg gtagtcttag
    15121 taagcgggga agcttatgac taagagcacc acgatgatga gtagcttcat catgaccctt
    15181 tccttattta aggccccttc ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc
    15241 gtcggcttac ctcctttcgc catgcactta gtctatcgct gacttgcaga taaaatagtt
    15301 tccagtgtga ttattgcgta acgtctcgca aagaattttt gctggcagga tcgcagacgg
    15361 caaagcctgc ggattgacaa tcttatcgtg aaggcatact ttcaggagtg agggtagagc
    15421 ggggtttccc ccgccctggt agtcttagta tgctgggtag ctaatcacta agagcaccac
    15481 gatgatgagt agcttcatca tgaccctttc cttatttaag gccccttcct cgggaggggc
    15541 tttcccgttt cagcatccgg ctgaaatctg cggcttacct cctttcgccc tgtatgcagt
    15601 ctatcgctaa cgcgtagata aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa
    15661 tgtgcccgcc attcagacga ttcccgatag tgtttcataa ttcctccatt tttctccctt
    15721 attggctggc tacactagta tcattccgcg aaacgtttca ggaagagaaa ctcttaacga
    15781 tgaaaggcag ttataaatcc cgttgggtaa tcgtaatcgt ggtggttatc gccgccatcg
    15841 ccgcattctg gttctggcaa ggccgcaatg actcccggag tgcagcccca ggggcgacga
    15901 aacaagcgca gcaatcgcca gcgggtggtc gccgtggtat gcgttccggc ccattagccc
    15961 cggttcaggc ggcgaccgcc gtagaacagg cagttccgcg ttacctcacc gggcttggca
    16021 ccattatcgc cgctaatact gttacggtgc gcagccgcgt ggacggtcaa ctgatggcgt
    16081 tacatttcca ggaaggccag caggtcaaag caggcgattt actggcagaa attgacccca
    16141 gccagttcaa agttgcatta gcacaaaccc agggccaact ggcaaaagat aaagccacgc
    16201 ttgctaacgc ccgccgtgat ctggcgcgtt atcagcaact ggcaaaaacc aatctcgtct
    16261 cccgtcagga gctggatgcc caacaggcgc tggtcagtga aaccgaaggc accattaagg
    16321 ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga ctggagccgc atcaccgcac
    16381 cagtcgatgg tcgcgttggt ctcaagcagg ttgatgttgg taaccaaatc tccagtggtg
    16441 ataccaccgg aattgtggtg atcacccaga cgcatcctat cgatttagtc tttaccctgc
    16501 cggaaagcga tatcgctact gtagtgcagg cgcaaaaagc cggaaaaccg ctggtggtag
    16561 aagcctggga tcgcaccaat tcgaagaaat taagtgaagg cacgctgtta agcctcgata
    16621 accaaatcga tgccacaacc ggtacgatta aagtgaaagc acgctttaat aatcaggatg
    16681 atgcgctgtt tcccaatcag tttgttaacg cgcgcatgtt agtcgacacc gaacaaaacg
    16741 ccgtagtgat cccaacagcc gccctgcaaa tgggcaatga aggccatttt gtctgggtgc
    16801 tgaatagcga aaacaaggtc agcaaacatc tggtgacgcc gggcattcag gacagtcaga
    16861 aagtggtgat ccgtgcaggt atttctgcgg gcgatcgcgt ggtgacagac ggcattgatc
    16921 gcctgaccga aggggcgaaa gtggaagtgg tggaagccca gagcgccacc actccggaag
    16981 agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg ctcctgatgc aggtgttacc
    17041 cccgagcagc acaggcggcc cgtcgcgcct gtttattatg cgtcctgtct ccaccacgct
    17101 gctgatggtg gcgatcttac tcgccgggat tatcggttat cgcgccctgc ccgtttcggc
    17161 gctgccggaa gtggactatc cgaccattca ggtggtcacg ctctacccag gtgccagccc
    17221 ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc cagttcgggc agatgtctgg
    17281 cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca gttatcactt tgcagttcca
    17341 gctaacatta ccgctcgatg tcgccgagca ggaagtgcag gccgcgatta acgctgcgac
    17401 caacttgttg ccgagcgatc tgcctaaccc gccggtttac agcaaagtga acccggcaga
    17461 tccgccgatc atgacgctcg ccgtcacctc aaccgccatg ccgatgacgc aagtggaaga
    17521 tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt tccggcgtcg gcctggtgac
    17581 gctttccggc ggtcagcgtc cggctgttcg cgtcaaactt aacgctcagg cgattgccgc
    17641 cctcggcctg accagcgaaa ccgtgcgcac cgccattacc ggcgctaacg ttaactcggc
    17701 aaaaggtagc ctcgacggcc cttcccgtgc ggtcacgctt tccgcgaacg accagatgca
    17761 atccgccgaa gagtatcgcc agctaatcat cgcctaccag aacggcgcgc caattcgtct
    17821 gggcgatgtc gcaactgtag agcaaggtgc agaaaacagc tggctcggcg cgtgggcgaa
    17881 caaagaacag gccattgtga tgaatgttca gcgccagccc ggtgctaaca ttatctccac
    17941 cgccgacagc attcggcaga tgctgccaca gctcactgag agtctgccga aatcggtgaa
    18001 ggtgacagtg ctttccgatc gcaccaccaa tatccgcgca tccgtcgatg atactcagtt
    18061 tgaattgatg atggctatcg cgctggtagt catgattatc tacctgtttt tgcgcaatat
    18121 tccggcgacc atcattcccg gtgttgctgt accgctgtcg ttaatcggca ctttcgcggt
    18181 tatggtgttt ctcgattttt caatcaataa cctgacactg atggcgttaa ctatcgccac
    18241 cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac atttcccgct atatcgaaaa
    18301 aggcgaaaaa ccgttggcgg cggcgctcaa gggcgcaggt gaaatcggct ttaccattat
    18361 ctcgctgacc ttctcactga ttgcggtgtt gatcccactg ctgtttatgg gcgatatcgt
    18421 cgggcgactg ttccgcgaat ttgctattac gctggcggta gcgattttga tctcagcggt
    18481 ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg ctcagccagg agtcgttgcg
    18541 taaacagaac cgcttctccc gtgcctcgga aaaaatgttc gacaggataa tcgccgccta
    18601 tggtcgtgga ctggcgaagg tgctgaatca tccgtggctg accttaagcg tggcgctcag
    18661 cacgctgctg cttagcgttc tcttatgggt gttcattccg aaaggtttct tcccggtaca
    18721 ggacaacggc attattcagg gcactttgca ggcaccgcaa tccagctcct ttgccaatat
    18781 ggcccagcga caacgccagg tcgcggacgt gattttgcag gatccggcag tgcaaagcct
    18841 gacctcattt gttggcgttg atggcactaa cccgtcgctg aatagtgcac gtttacaaat
    18901 caacctcaaa ccgctggatg aacgtgatga ccgagtgcaa aaagtcattg cccgtctgca
    18961 aacggcggtg gataaagtgc cgggcgtcga tctcttcctg caaccaacgc aggatctgac
    19021 tattgatact caggtcagcc gcacccagta ccagtttacc ttgcaggcca cctcactgga
    19081 tgcgctcagt acctgggtgc cacagttgat ggaaaaactc cagcaactgc cacagctttc
    19141 tgatgtctcc agcgactggc aggacaaagg gctggtggcg tatgtcaatg ttgatcgcga
    19201 cagcgccagc cgtctgggga tcagcatggc ggatgtcgat aacgccctgt acaacgcgtt
    19261 tggtcagcga ctgatttcca ctatttatac tcaggctaac cagtatcgcg tagtgctgga
    19321 gcacaacacc gaaattaccc cgggcctcgc ggcgctggat accattcgcc tgaccagtag
    19381 cgacggcggc gtggtgccgt taagctcaat tgccaaagtt gaacagcgtt ttgcgccgct
    19441 ttccatcaac catctggatc agttcccggt aacgaccatc tcctttaacg tgccggataa
    19501 ctattcgctg ggcgatgcgg tgcaggcgat tatggacacc gaaaagacgc tgaatctgcc
    19561 ggtggatatc accacgcaat ttcagggcag caccctcgcc ttccagtcgg cgctgggcag
    19621 cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat atcgtgctcg gcattctgta
    19681 cgagagcttt attcacccga tcaccattct ctcgacgcta cccaccgcag gggtcggcgc
    19741 gctgctggca ttaatgattg ccggtagcga actggatgtg attgcgatta tcggcattat
    19801 tttgctgatc ggtattgtga agaagaacgc catcatgatg atcgacttcg cgctggctgc
    19861 tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac caggcttgtc tgttgcgttt
    19921 tcgtccgatc ctgatgacca ccctggcggc tctgcttggc gcgctgccgc tgatgttgag
    19981 taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc ggcatggtcg gcggtctgat
    20041 tgtcagccag gtgctgacgc tgttcaccac gccggtgatt tatctgctgt tcgaccgcct
    20101 ggcattgtgg accaaaagtc gctttgctcg ccatgaagag gaggcgtaag tgaagttttt
    20161 tgccctcttc atttaccgcc cggtggcgac gattttactg tcggtcgcca ttaccctgtg
    20221 cggcatactg ggcttccgta tgctgccggt cgccccgctg ccgcaggtcg attttccggt
    20281 gattatggtc agcgcctcgc tgcccggtgc gtcaccagaa acaatggcgt cttccgttgc
    20341 cacgccgctg gagcgctcac ttgggcgcat tgccggagtc agtgaaatga cctccagcag
    20401 ttcgctcggc agcacgcgta ttattttgca gtttgatttt gaccgggata tcaacggcgc
    20461 ggcgcgcgat gtgcaggcgg cgatcaacgc tgcacaaagt ttgctgccca gcgggatgcc
    20521 cagccgtccg acctatcgca aagctaaccc gtcggatgcg ccaattatga tcctcacgct
    20581 gacatccgat acttattcgc agggtgaact gtacgatttt gcctcgacgc agctggctcc
    20641 gacgatttcg caaatcgacg gtgtcggtga tgtcgatgtt ggcggcagct cgctgcccgc
    20701 cgtacgcgtc gggctgaatc cgcaggcgct gtttaatcag ggcgtctcgc tggacgacgt
    20761 acgcaccgcc atcagcaatg ccaacgtgcg taaaccgcag ggggcgctgg aagatggcac
    20821 tcaccgctgg cagatccaga ccaatgatga gctaaaaacc gccgccgaat atcagccgtt
    20881 gattattcat tacaacaacg gcggcgcggt tcgtctgggc gatgtggcga cggtgaccga
    20941 ctcggtgcag gatgtgcgca acgccgggat gaccaacgcc aaaccggcta ttttgctgat
    21001 gatccgcaag ctgccggaag ccaatattat ccagacggta gacagcatcc gggcaaaatt
    21061 accggagttg caggaaacca ttccggcggc gattgatctg caaattgctc aggatcgctc
    21121 ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg ctgattatct cggtggcgct
    21181 ggtgattctg gtggtgtttt tattcctgcg ctcgggtcgc gccactatta ttcccgccgt
    21241 tgcggtgccg gtttcgctga ttggtacgtt tgcggcgatg tacctgtgcg gattcagtct
    21301 caataacctt tcgttaatgg cgctcaccat cgctactggt ttcgtggtgg atgacgccat
    21361 cgtggtgctg gaaaacattg cccgtcatct ggaagcgggg atgaaaccgc tacaggccgc
    21421 gctacaaggt actcgcgaag tcggctttac ggtgctatcg atgagtctgt cactggtggc
    21481 ggtgttcctg ccgctgctgt tgatgggcgg attgccgggc cgactgttac gcgaatttgc
    21541 cgtgacgctt tctgtcgcca ttgttatatc gttgctggtt tctctgacat taacgccaat
    21601 gatgtgtggc tggatgctga aagccagcaa gccgcgtgag caaaagcgac tgcgtggttt
    21661 tggccgcatg ttggtagccc tgcaacaagg ctacggcaag tcgctgaaat gggtgctcaa
    21721 tcatacccgt ctggtgggtg tggtgctgct tggcaccatt gcgctgaata tctggctgta
    21781 tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtgctga tgggcgggat
    21841 acaggcggat cagagcattt cgtttcaggc gatgcgcggc aaattgcagg atttcatgaa
    21901 aattatccgt gacgatccgg cagtggataa tgtcaccggc tttaccggcg gttcacgagt
    21961 taacagtggg atgatgttta tcaccctcaa gccacgcgac gaacgcagcg aaacggcgca
    22021 gcaaattatc gaccgtctgc gcgtaaaact ggcgaaagaa ccgggggcga atctgttcct
    22081 gatggcggta caggatattc gcgttggtgg gcgtcagtcg aacgccagct accagtacac
    22141 gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc gcaaaaaact
    22201 ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag caggataacg gcgcggagat
    22261 gaatctggtt tacgaccgtg acaccatggc acggctggga atcgacgtac aagccgccaa
    22321 cagtctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc agccgatgaa
    22381 ccagtataaa gtggtgatgg aagtggatcc gcgctatacc caggacatca gtgcgctgga
    22441 aaaaatgttc gttatcaata acgaaggcaa agcgatcccg ctgtcgtatt tcgctaaatg
    22501 gcaaccggcg aatgccccac tatcggtgaa tcatcaggga ttatcggcgg cctcgaccat
    22561 ttcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga tcgatcgcgc
    22621 aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggaa cagcgcaggt
    22681 gttccaggag acgatgaact cacaggtgat cctgattatc gccgccatcg ccacggtgta
    22741 tatcgtgctg ggtatccttt acgagagtta tgtgcacccg ctgacgattc tctccaccct
    22801 gccctcggcg ggtgttggag cgctgctggc gctggagctg ttcaatgccc cgttcagcct
    22861 aatcgccctg atagggatca tgctattaat cggcatcgtg aagaaaaacg ccattatgat
    22921 ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg aagctatttt
    22981 ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgact accctggcgg cgctgtttgg
    23041 cgcgctgccg ctggtgttgt cgggcggcga cggctcggag ctgcggcaac ccctggggat
    23101 caccattgtc ggcggactgg taatgagcca gctccttacg ctgtatacca cgccggtggt
    23161 gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac aaacggtaac
    23221 cgagtaaatg acagatcttc ccgacagcac ccgttggcaa ttgtggattg tggctttcgg
    23281 cttctttatg cagtcgctgg acaccaccat cgtaaacacc gcccttccct caatggcgca
    23341 aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg tgctgaccgt
    23401 ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc gcaatatttt
    23461 ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt ccggcacgct
    23521 gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggcgcgatga tggtgccggt
    23581 cggcagattg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg cgatgacctt
    23641 tgtcacgtta cccggtcagg tcggtccgct gctcggtccg gcgctcggcg gtctgctggt
    23701 ggagtacgcg tcgtggcact ggatcttttt gatcaacatt ccggtgggga ttatcggtgc
    23761 gatcgccaca ttgatgttaa tgccgaacta caccatgcag acgcggcgct ttgatctttc
    23821 cggcttttta ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg acggcagtaa
    23881 aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg tggtggcact
    23941 ggtgctttat ctgctgcacg ccagaaataa caaccgcgcc ctgttcagtc tgaaactgtt
    24001 ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta ttggcagtgg
    24061 catgttgccc tttatgacac cggttttcct gcaaattggc ctcggtttct cgccgtttca
    24121 tgccggactg atgatgatcc cgatggtgct tggcagcatg ggaatgaagc gaattgtggt
    24181 tcaggtggtg aatcgctttg gttatcgtcg ggtactggta gcgaccacgc tgggtctgtc
    24241 gctggtcacc ctgttgttta tgactaccgc tctgttgggc tggtactacg ttttgccgtt
    24301 cgtcctgttt ttacaaggaa tggtcaactc aacgcgtttc tcctccatga acaccctgac
    24361 gctgaaagat ctcccggaca atctggcgag cagcggaaac agcctgctgt cgatgattat
    24421 gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg ttgctgggac tttttggttc
    24481 gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca cctggcttag
    24541 catggcgttg atcatcgccc ttccggcgtt catctttgcc agagtgccga acgatacgca
    24601 tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg gcgacccggt
    24661 attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttgct gatcagtatg
    24721 cactgggcgg tgcgtatcag ttttgagcgt ggctttattg attacatcaa gcatggtaat
    24781 gaacaacgat tacaactgtt aagtgatgcg cttggcgagc agtatgcgca gcatggcaac
    24841 tggcgcttcc tgcgcaacaa tgatcgcttt gtctttcaga tcctgcgttc atttgaacac
    24901 gataattcgg aagataaacc cggcccgggt atgccaccgc acggctggcg tacccagttc
    24961 tgggtggttg atcaaaacaa caaagtgctg gttggtccgc gagcgccgat tccacctgac
    25021 ggtacacggc gacccattct ggtcaacggt gcggaagttg gcgcggtgat cgcctccccc
    25081 gttgagcggt taacgcgcaa tactgatatc aatttcgata aacaacagcg gcaaaccagc
    25141 tggctgatcg tcgccctggc aacgttactc gcggcgctcg ccacctttct gctggcgcgc
    25201 ggtttgctgg caccggtaaa acgacttgtc gatggcacgc acaaactggc ggcgggcgat
    25261 ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg gcaaactggc gcaagacttc
    25321 aaccagcttg ccagcacgct ggagaaaaac cagcaaatgc ggcgcgattt tatggccgat
    25381 atttcacacg aactgcgtac gccattagcg gtgctgcgcg gtgaactgga agccattcaa
    25441 gatggcgtgc gcaaattcac gccggagacg gtggcgtctt tacaggcgga agtcggtaca
    25501 ctgactaaac tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg cgctctcgcc
    25561 tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggcgg cgcatttcgc
    25621 gaacgatttg ccagccgtgg cctgaaactg cagttttccc tgccagacag tattaccgta
    25681 tttggcgatc gcgaccgttt aatgcagtta ttcaataact tactcgaaaa cagcctgcgc
    25741 tacactgata gcggcggcag cctgaaaatc tctgccgagc agcacgacaa aacggtgcgc
    25801 ctgacctttg ccgacagtgc gccaggtgtc agtgacgagc agctacaaaa attatttgaa
    25861 cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gtggttccgg gctggggctg
    25921 gcgatttgcc tgaacattgt tgaagcacat aatggccgca ttattgctgc ccattcgcct
    25981 tttggcgggg taagcattac agtagagtta ccgctggaac gggatttaca gagagaagta
    26041 tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa gatgaaccga
    26101 agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgccg acgcttatca
    26161 gccacggcga tcaggtactg ccgtatgtgc gccagacacc accggatctg atcctgttag
    26221 atctgatgct ccctggcacc gatggcctga cgctgtgccg ggaaattcgt cgtttttctg
    26281 acattccgat cgtgatggtg acggcaaaaa tcgaagagat cgatcgcctg ctggggctgg
    26341 agattggcgc agatgattat atctgtaagc cgtacagccc acgggaagtg gtagcgcgcg
    26401 tcaaaaccat tttgcgccgt tgcaaaccgc agcgcgagtt gcagcaacag gatgctgaaa
    26461 gcccgttgat tatcgacgaa ggtcgttttc aggcttcatg gcgcggtaaa atgcttgacc
    26521 tgacgcctgc ggaatttcgt ctgctgaaaa cgctctctca tgaaccagga aaagtgttct
    26581 cccgcgagca tttgctcaat catctttatg acgactaccg cgtagtaacc gaccgcacca
    26641 tcgacagcca cattaaaaac ctgcgccgca agctggaatc tctcgacgcc gaacagtcat
    26701 ttatccgcgc cgtttacggc gttggttacc gctgggaagc cgacgcctgc cgcatcgttt
    26761 agttttagcg acatgacttt gttagccgga gatggcgttc agaccagatc cggcaacatt
    26821 atcccacgca tggtcagcaa actgacatta tctctccgcc ctggcaggca tcagaaaatc
    26881 gctcatactt taatcggtaa acagcacctt tagatgctgt tttcgatacg caatttcaat
    26941 caaggagtca ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg
    27001 tttgtgctaa aagcgggcaa tggtgagact atcctcacca gcgagctgta tacctcaaaa
    27061 gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga agaacgctat
    27121 gagaaaaaaa cggcaagtaa cggcaaattc tatttcaatc tgaaagccgc taatcatcaa
    27181 attatcggct ccagccagat gtacgccacc gcgcaatctc gtgaaaccgg aattgcctcc
    27241 gttaaagcca atggcacaag ccagacggtg aaagacaata cgtaattacg atgccgggcg
    27301 tgggttgttg gcgtccggct ttatatccaa ataacgctta gttattaata attaagagta
    27361 accgccgcca ttaaaatagt gggcactgac gagcactcgt tgtgctttct ttgtttacca
    27421 tcccatactt atcctcctga acatgttcat gtgccaaaat atcttctaat actttcgtga
    27481 cgctatccgg tttatgacat acactgatgt aagattttta cttacaggcg ggcgtaaaat
    27541 ggccagaacg atgacggttg atctcggcga tgagttgcgt gaatttatcg agtccttaat
    27601 tgaatccggc gactatcgca cccaaagcga agtgatccgt gagtcactgc gattacttcg
    27661 agaaaagcaa gcagagtctc gcctgcaagc gttacgtgac atgctcgcag aagggttgag
    27721 tagtggtgaa gctcagccgt gggaaaagga cgcgttctta cggaaggtga aagcagggat
    27781 aaggaaataa tgtgcgtatt attaaactca tgccaaaagc caatgaggat ttagaaggca
    27841 tctggtatta cagctatcac catttcggcg aaccacaagc cgacagatat gtggaacatc
    27901 tttcagacgt tctccagatt ctgagtaaca ataatatagg tacgcccaga cctgaattag
    27961 gcgaaggtat ttttgttctt ccttttgaac gccacgtcat ttatttcctg caatcaccag
    28021 gggaaattat cgtcattcgt atccttaacc aaaaccagga cgccaccaga catttacact
    28081 ggagctaaac ccgccgattg cgctacaatg cccgccctta aagtgggggc actcccctaa
    28141 ccgcttcatc aggtgaagcg gatctgacct gtcatcagaa cgagagaatt atgtttaaac
    28201 cggaactcct ttccccggcg ggaacgctga aaaatatgcg ttacgctttc gcttatggcg
    28261 cagatgctgt ttatgcgggc cagccgcgtt attccctgcg tgtgcgcaac aacgaattca
    28321 accacgaaaa tcttcagctc ggcatcaatg aagcccacgc gctggggaaa aagttttatg
    28381 tcgtggtcaa cattgcaccg cacaacgcca agctgaaaac ctttatccgt gacctgaaac
    28441 cggtggtgga aatggggccg gatgcgctga ttatgtccga tccagggctg attatgctgg
    28501 tgcgtgagca cttccctgaa atgccgatcc acctttcggt gcaggctaac gccgtgaact
    28561 gggcgacggt gaaattctgg cagcaaatgg gcctgacccg cgtgatcctc tctcgcgagc
    28621 tgtcgctgga agagattgaa gagatccgca atcaggtgcc ggatatggag atcgagatct
    28681 tcgttcacgg cgcgctgtgc atggcctact ccggtcgctg cctgctctct ggctatatca
    28741 acaagcgcga cccgaaccag ggcacctgca ccaacgcctg ccgctgggag tacaacgtcc
    28801 aggaagggaa agaagatgat gttggcaaca tcgtacacaa gtacgagccg attccggtgc
    28861 aaaatgttga gccgacgctg ggtatcggcg caccaaccga caaagtgttt atgatcgaag
    28921 aggcccagcg tccgggcgag tatatgaccg cgtttgaaga tgagcacggc acttacatca
    28981 tgaactcgaa agatctgcgc gccatcgccc atgtagaacg cctgaccaaa atgggcgtgc
    29041 attcgctgaa aatcgaaggt cgtaccaaat ctttctacta ttgtgcacgc accgcacagg
    29101 tttaccgcaa agctatcgat gacgccgctg cgggaaaacc gttcgatacc agcctgctgg
    29161 aaactctgga aggtctggcg catcgtggct ataccgaagg tttcctgcgt cgtcatactc
    29221 acgacgatta tcagaactac gaatacggtt attcagtttc tgaccgccag cagtttgttg
    29281 gtgagtttac cggtgagcgc aagggggacc tcgcggcggt agcggtgaaa aataaattct
    29341 ccgttggcga cagccttgag ctgatgacgc cgcaaggcaa cattaatttt acccttgagc
    29401 acatggaaaa cgccaaaggc gaagctatgc cgatagcacc aggcgatggt tatactgtgt
    29461 ggctcccggt cccgcaggat cttgagctca attacgcgct gctgatgcgt aatttctccg
    29521 gggaaaccac gcgtaatccc cacggtaagt gattaatttc gattattttt cccggatgga
    29581 aaattcttag aaaccgatca catacagctg catttattaa ggttatcatc cgtttcgctg
    29641 aaaaacataa cccataaaat gctagctgta ccaggaacca cctccttagc ctgtgtaatc
    29701 tcccttacac gggcttattt tttatgcata agccctatcc ctggtcaccg tcttccattg
    29761 accacatcga tagaatctcc cttcatagca cgatgccttt cacgtaacgg catcgtgctc
    29821 gcacaggttc cggctaagca caaccagaac gcgcatgttt gacgcttacc aaaaaatatt
    29881 ctcactctcc acatttgaat gtcagacgag cgacgccatg taatcctgca ccttctgtct
    29941 tcaggtcaac tatctgcatt tttttgccct gagtaacaca gaaatgggct gcatcatttt
    30001 ttactatatt ttctgcacca gatattctgc ccctggctaa agaagcttcg gcttcggtgt
    30061 agtattggtt atcgagttta cgctgaatat tacttttata tgcaagacca aatttaccga
    30121 tacttgtctc atcattatgc acagcacaac cagacataat aaaaatacta attaatgata
    30181 tagcagctat ctttttcatc tcaccttccc ccattaaata ccaacgacac tctctag
//
LOCUS       DAZFKM010000054        29667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFKM010000054 DAZFKM010000000
VERSION     DAZFKM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(69..1898)
                     /gene="glmS"
                     /locus_tag="ACHKAY_002443"
     CDS             complement(69..1898)
                     /gene="glmS"
                     /locus_tag="ACHKAY_002443"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO5701110.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2060..3430)
                     /gene="glmU"
                     /locus_tag="ACHKAY_002444"
     CDS             complement(2060..3430)
                     /gene="glmU"
                     /locus_tag="ACHKAY_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO5701111.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3781..4200)
                     /gene="atpC"
                     /locus_tag="ACHKAY_002445"
     CDS             complement(3781..4200)
                     /gene="atpC"
                     /locus_tag="ACHKAY_002445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO5701112.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4221..5603)
                     /gene="atpD"
                     /locus_tag="ACHKAY_002446"
     CDS             complement(4221..5603)
                     /gene="atpD"
                     /locus_tag="ACHKAY_002446"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO5701113.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5630..6493)
                     /gene="atpG"
                     /locus_tag="ACHKAY_002447"
     CDS             complement(5630..6493)
                     /gene="atpG"
                     /locus_tag="ACHKAY_002447"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO5701114.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6544..8085)
                     /gene="atpA"
                     /locus_tag="ACHKAY_002448"
     CDS             complement(6544..8085)
                     /gene="atpA"
                     /locus_tag="ACHKAY_002448"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO5701115.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8098..8631)
                     /gene="atpH"
                     /locus_tag="ACHKAY_002449"
     CDS             complement(8098..8631)
                     /gene="atpH"
                     /locus_tag="ACHKAY_002449"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO5701116.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8646..9116)
                     /gene="atpF"
                     /locus_tag="ACHKAY_002450"
     CDS             complement(8646..9116)
                     /gene="atpF"
                     /locus_tag="ACHKAY_002450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO5701117.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9178..9417)
                     /gene="atpE"
                     /locus_tag="ACHKAY_002451"
     CDS             complement(9178..9417)
                     /gene="atpE"
                     /locus_tag="ACHKAY_002451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO5701118.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9464..10279)
                     /gene="atpB"
                     /locus_tag="ACHKAY_002452"
     CDS             complement(9464..10279)
                     /gene="atpB"
                     /locus_tag="ACHKAY_002452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO5701119.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10288..10668)
                     /gene="atpI"
                     /locus_tag="ACHKAY_002453"
     CDS             complement(10288..10668)
                     /gene="atpI"
                     /locus_tag="ACHKAY_002453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO5701120.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11285..11908)
                     /gene="rsmG"
                     /locus_tag="ACHKAY_002454"
     CDS             complement(11285..11908)
                     /gene="rsmG"
                     /locus_tag="ACHKAY_002454"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO5701121.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11972..13861)
                     /gene="mnmG"
                     /locus_tag="ACHKAY_002455"
     CDS             complement(11972..13861)
                     /gene="mnmG"
                     /locus_tag="ACHKAY_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO5701122.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14239..14682)
                     /gene="mioC"
                     /locus_tag="ACHKAY_002456"
     CDS             complement(14239..14682)
                     /gene="mioC"
                     /locus_tag="ACHKAY_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO5701123.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14772..15230)
                     /gene="asnC"
                     /locus_tag="ACHKAY_002457"
     CDS             complement(14772..15230)
                     /gene="asnC"
                     /locus_tag="ACHKAY_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO5701124.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15382..16374
                     /gene="asnA"
                     /locus_tag="ACHKAY_002458"
     CDS             15382..16374
                     /gene="asnA"
                     /locus_tag="ACHKAY_002458"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO5701125.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16379..17830)
                     /gene="viaA"
                     /locus_tag="ACHKAY_002459"
     CDS             complement(16379..17830)
                     /gene="viaA"
                     /locus_tag="ACHKAY_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO5701126.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17824..19320)
                     /gene="ravA"
                     /locus_tag="ACHKAY_002460"
     CDS             complement(17824..19320)
                     /gene="ravA"
                     /locus_tag="ACHKAY_002460"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO5701127.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19543..21411
                     /gene="kup"
                     /locus_tag="ACHKAY_002461"
     CDS             19543..21411
                     /gene="kup"
                     /locus_tag="ACHKAY_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO5701128.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21578..21997
                     /gene="rbsD"
                     /locus_tag="ACHKAY_002462"
     CDS             21578..21997
                     /gene="rbsD"
                     /locus_tag="ACHKAY_002462"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO5701129.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            22005..23510
                     /gene="rbsA"
                     /locus_tag="ACHKAY_002463"
     CDS             22005..23510
                     /gene="rbsA"
                     /locus_tag="ACHKAY_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO5701130.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23515..24480
                     /gene="rbsC"
                     /locus_tag="ACHKAY_002464"
     CDS             23515..24480
                     /gene="rbsC"
                     /locus_tag="ACHKAY_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO5701131.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24505..25395
                     /gene="rbsB"
                     /locus_tag="ACHKAY_002465"
     CDS             24505..25395
                     /gene="rbsB"
                     /locus_tag="ACHKAY_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO5701132.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25521..26450
                     /gene="rbsK"
                     /locus_tag="ACHKAY_002466"
     CDS             25521..26450
                     /gene="rbsK"
                     /locus_tag="ACHKAY_002466"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO5701133.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26454..27446
                     /gene="rbsR"
                     /locus_tag="ACHKAY_002467"
     CDS             26454..27446
                     /gene="rbsR"
                     /locus_tag="ACHKAY_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO5701134.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27412..28839)
                     /gene="mdtD"
                     /locus_tag="ACHKAY_002468"
     CDS             complement(27412..28839)
                     /gene="mdtD"
                     /locus_tag="ACHKAY_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO5701135.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28862..29554)
                     /gene="yieP"
                     /locus_tag="ACHKAY_002469"
     CDS             complement(28862..29554)
                     /gene="yieP"
                     /locus_tag="ACHKAY_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO5701136.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7140 a         7612 c         7568 g         7347 t
ORIGIN      
        1 tgaatttatt gatgtcagtt ataaaaaagg gccaccggtc ggcagccctt ttttatctat
       61 gatagtgatt actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg
      121 cctttgatca gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt
      181 gcaatcacct cttccacatg cggcatctcg atgatgtgca tgttatcgct acttacaaaa
      241 cccgcatcct gatcggcgaa gacatacaac tgaccgccac gcgcgcgaac ttcttcaatg
      301 ttggatttca gtttttccaa cagttcgttg ttcggtgcaa cgacgataac cggcatatcc
      361 gcgtcaatca gcgccagcgg gccgtgtttc agctcgcctg cagcgtaggc ttcagcgtga
      421 atgtaagaga tctctttcag cttcaatgcg ccttccagcg cgattgggta ctgatcgcca
      481 cggcccagga acagcgcgtg atgtttgtca gagaaatctt ctgccagagc ttcaatgcgt
      541 ttgtcctgag acagcatctg ctcaatacgg ctcggcagcg cctgcagacc atgcacaatg
      601 tcatgttcaa tggaggcatc cagacctttc aggcgagaca gcttcgccac cagcatcaac
      661 agcacagtta actgagtggt gaatgcttta gtggatgcca cgccgatttc cgtacccgcg
      721 ttggtcatca gcgccagatc ggattcgcgc accagagaag aacccggaac gttacagatt
      781 gccagcgaac caaggtaacc cagctctttc gacagacgca ggcccgccag ggtatccgcg
      841 gtttcgccag actgtgacaa ggtgatcatc aggctgttac gacgcacggc agatttgcga
      901 tagcggaatt cagaggcgat ttcgacgtcg cacggaatac ctgccagcga ttcaaaccag
      961 taacgggaaa ccataccgga gttataagaa gtaccacagg cgaggatctg aatatgctca
     1021 accttcgaca gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac ctgaccgtgg
     1081 ctgatgcgcc cggtaagggt gtttttgatc gcgttcggct gttcgtagat ctctttctgc
     1141 atgtagtgac ggtaaatacc tttatcgccc gcgtcatatt gcagattgga ttcgatatcc
     1201 tgacgtttta cttccgcgcc agttttatcg aagatgttta ccgaacggcg agtaatttcc
     1261 gcaatatcgc cctcttcaag gaagataaag cgacgggtca ccggcagcag cgccagctgg
     1321 tcagaagcga taaagttttc gcccataccc agaccaatca ccagcggact accagaacgt
     1381 gccgccagca gggtatccgg gtgacgggag tccatgatca ctgtaccgta cgcaccacgc
     1441 agttgcggga tagcacgcag aacagcctca cgcagagtac cgccctgttt cagctcccag
     1501 ttcaccagat gggcaattac ttcggtgtct gtttcagaaa cgaaggtata gccacgcgct
     1561 tttaactctt cgcgcagcgg ttcatggttt tcgatgatgc cgttatgcac caccacaatg
     1621 tgttcagaaa catgcggatg cgcattcact tctgaaggtt caccgtgggt cgcccagcga
     1681 gtatgagcaa tacccgtacc gccatgcaga ggatgttctt ccgctgcctg agccagcatc
     1741 tggactttac cgaggcgacg caggcgggtc atatgacctt ccgcatcaac aacggccaga
     1801 ccggcagagt catatccgcg gtattccaga cgacgtaaac cttcaagaag gatttctgct
     1861 acatcacgtt gcgcgatcgc gccaacaatt ccacacatag tttttgattc cgatttatat
     1921 cgttgtcggt caacctgtat gcccgttttt tcgggcgccc cgagccttgt agagagtggg
     1981 gttattttta tagttactgc ttgtgggagg gagattatgt tatctcctca tcccatgtga
     2041 ccggattacc cggccagaat cactttttct ttaccggacg acgccagcct tctttctgag
     2101 tctgcggcac acggctgata gctaatgcat tttcgccgac attacgcgtc acagttgtac
     2161 ccgcagcaat ggtcgcgcct ttgcctactg ttaccggggc caccagctga gtgtcggaac
     2221 cgacaaacac atcgtcgccg ataatggtct taaatttatt cgcaccatcg tagttgcagg
     2281 taatggttcc cgcgccgatg ttaacgttat cgccaatttc cgcatcgccc aggtaagtca
     2341 gatgaccagc tttcgagcct ttacccagac gcgctttttt catctcaaca aagttaccga
     2401 cgtgtgcacc ttccagcaac tcagcaccag gacgcagtcg ggcaaacggg ccaatagtac
     2461 aggccgctgc cagattcgcg tcttccacaa cggtatacgg actgatttcg caatcatcac
     2521 caatcacgct gtttttaatc acgcaaccgg tgccgatttt cacgcgatga ccgagagtca
     2581 cgttgccctc gatgataacg ttagtatcaa tttcaacatc gcgcccgtga gtaagcgtac
     2641 cgcgcaaatc aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc agtttttcag
     2701 cctgttcgga ctggtaaacg cgctccagac gggagagttg caggcggtta ttcacacctt
     2761 ctacttcgct taaacgttgc ggatgaacgg cgacgatttc acgcccttcc tgatacgcca
     2821 gcgcaataat gtcggtgatg tagtattcgc cctgagcatt attgttggtc agcttcgcca
     2881 gccagcgttt catatctgcg ccgttagcga tcagaatgcc ggtgttgatc tcctgaatct
     2941 gacgctgctc gtcggtggca tctttgtgct caacaatgcc ggtaactttg ccattttcac
     3001 gggtgatacg tccataaccg gtcggatcat ccagtttcac cgtcagcaga ccaatgccac
     3061 cctgcggttt agcatcacgc aggcgctgga gtgtttcgac agagatcagc ggcacgtcgc
     3121 cgtagagcat taaaatgtct tcatcatcgg caaagaaagg tgcggcctgc tgcattgcat
     3181 gacccgtacc cagctgctct gcctgaagca cccagttaag gttgtcgtct ttcagcgcct
     3241 gtttaagcag atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg cctaattcat
     3301 tcgcagcatc aatgacatgc tgaaccatcg ctttcccggc aagggtatgc agcactttcg
     3361 gaagatcgga atacatgcgc gtgcctttgc ctgcggcaag gatcactacg ctcatagcat
     3421 tattcaacat acgcgtcctg actgtaattt gagaacgaat ttaaaccgct tcaccttgaa
     3481 aaaactacat ttttttcatc gtgaaatgga cagaggataa attgttcaat aacggattat
     3541 ccccgctgac gaaagtgcca ttttcgacca tcgtcacgcc ttttgtctgc agaaaattaa
     3601 ggcagtacct tcatttaagc gtcaggtgga tgtttttgct cttatttcga tcaatgaata
     3661 aacgggaaaa caggggtgtt ttatatcagc aggatctatg tgaacgctct tcaggacgga
     3721 tcacgcgtaa aaaaaagcca gcctgtttcc agactggctt ttgtgctttt caagccggtg
     3781 ttacatcgct tttttggtca actcgataac gcgcagctgc gcgatcgctt tggccagttc
     3841 cgcagacgcc tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct cttcagcctt
     3901 acgtttcgct tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg cggtgtcggc
     3961 cagaacggtc acgttgccag gctgcacttc aagaatgccg ccagacagat agataaactc
     4021 ttcgtgaccg tgctgtttca cgatgcgaat cataccaggc ttaatggcgg tgagcagcgg
     4081 tgcgtggcca gggtagatcc ccagttcacc ttcgctaccc gttacctgga ttttctcgac
     4141 cagaccagag aacatttgtt gctctgcgct gacgacgtcc aggtggtaag tcattgccat
     4201 atcaccctcc gattaaggcg ttaaagtttt ttggcttttt ccacagcttc ttcgatggaa
     4261 ccgaccatgt agaacgcctg ctccggcagg tgatcgtatt cgccttccat gatgccttta
     4321 aagccacgga tggtgtcttt cagggagacg tatttacccg gagaaccggt gaatacttct
     4381 gccacgaaga acggctggga caggaagcgc tggatcttac gagcacgcgc taccaccagt
     4441 ttgtcttctt cagacagttc atccataccc aggatggcga tgatgtcttt cagttcctga
     4501 taacgttgca ggatggactg aacgccacgc gcggtgtcgt agtgttcctg accaaccacc
     4561 agcgggtcca gctgacggct ggtggagtcc agcgggtcaa cggccgggta gatacccaga
     4621 gacgcgatct gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt ggttgccgga
     4681 gacggatcag tcaagtcatc cgcaggtacg tataccgcct gtacggaggt gatagaacca
     4741 gttttggtgg aggtgatacg ttcctgcaga acgcccatct cttccgccag ggtcggctga
     4801 taacctaccg ctgaaggcat acggcccagc agtgcggata cttccgtacc ggccagggtg
     4861 taacgataga tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg gaatttctca
     4921 gccatggtca gaccggtcag agcaacgcgc agacggtttc ccggcggctc gttcatctgg
     4981 ccatacacca gggatacttt gtcgataacg ttggagtcgg tcatttcgtg gtagaagtcg
     5041 ttaccctcac gagtacgttc acctacgccc gcaaacacag agtaaccgga gtgctcgatc
     5101 gcgatgttac gaatgagctc catcatgttt acggttttac ctacacccgc accaccgaac
     5161 agaccaactt taccgccctt agcgaacgga cacatcaggt cgataacttt gataccggtt
     5221 tccagcagtt cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg gtgaatcgcc
     5281 caacgctctt cttcaccgat ctcgcctttc atgtcgaccg gttcacccag tacgttcatg
     5341 atacggccca gagtcgcttt acctaccggg acttcgatcg ggtgttcgag gtcttttaca
     5401 tccagaccgc gacgcagacc gtcggaggaa cccattgcga tggtacgcac gataccgccg
     5461 ccgagctgct gctgaacttc cagcaccaga cgctcattac cattttgcac ctcaagagca
     5521 tcgtacacgc gcggtacggc atcctgaggg aattcgacgt caactacggc gccgattacc
     5581 tggacaatct ttccagtagc catcttaaat cctctacgaa ataacctgtt taaaccgcgg
     5641 cggcccccga gacgatctcg gtgagttcct gagtaatgct ggcctgacga gctttgttgt
     5701 ataccaactg cagctcttta atcaggctgc cgccattgtc ggtcgcggct ttcatcgcca
     5761 ccatacgggc ggcctgctcg ctggccaggt tttcaaccac gccctgataa acctgagatt
     5821 cgacataacg acgcagcagg gtatccagca acgccttcgg atcgggttcg tacaggtaat
     5881 cccaggattt atgtttcaga tcatcatcat ctgatgccgg taacggcagc agctggctga
     5941 tggtcggaac ctgagacatg gtgttaataa atttgttgct gacaatgtaa agcttgtcca
     6001 gacggccttc gtcgtaggcc tgcaacatca cttttaccgg accgatcagt tcggacaggg
     6061 aagggttatc ccccatgccg gtgacctggg caacaacatt gccgcccacg gagttgaaga
     6121 acgacacgcc tttcgagccg atcattgcga ggtcgcattg aacgcctttg tcggtccagg
     6181 tcttcatttc cgccagcagt tttttgaaca ggttaatgtt caaaccaccg cacaaaccac
     6241 ggtcggtcga caccaccagg tagcccacgc gtttaacgtc gcgttcttcc aggtatgggt
     6301 gcttatattc cagattaccg ctcgcaaggt gaccaatcac tttgcgcatg gtatctgcat
     6361 aaggacggct ggccgccatg cgatcctgcg atttacgcat tttggaagcg gcgaccatct
     6421 ccatcgcttt agtgatcttt tgcgtgttct ggacgcttgc gatcttacta cgtatctctt
     6481 ttgcgccggc catgagcttc tcctcaatgc cttgcggcct gccctaaggc aagccgccag
     6541 acgttaccag gattgggttg ctttgaagga atcgaggatg cctttcagct tgccttcgat
     6601 ttcgtcgttg tagccaccgg tctggttgat ctcttgcatc aacggagcgt gatcacggtc
     6661 gacgtaagcc agcagagcgg cttcgaagct gccgattttc gacagttcaa catccgccag
     6721 gtaaccacgt tctgctgcga acagaaccag agactgttgc gctacggaca tcggcgcata
     6781 ctgtttctgt ttcagcagtt cggtcacttt ctgaccgtgg tcaagctgct tacgtgttgc
     6841 atcgtcaagg tcggatgcaa actgagagaa cgctgccagt tcacgatact gtgccagagc
     6901 ggtacggata ccaccggaca gttttttcat gatcttggtc tgtgctgcac caccaacacg
     6961 ggatacggaa atacccgggt taaccgcagg acgaataccg gcgttgaaca ggttggtttc
     7021 caggaagatc tgaccatcgg taatggagat tacgttggtc ggaacgaacg cagaaacgtc
     7081 acccgcctga gtttcaataa tcggcagtgc ggtcagagaa ccggttttcc ctttcacttc
     7141 acctttggtg aaggcttcaa cgtattcggc gttaacacgc gcagcacgct ccagcagacg
     7201 agagtggagg tagaatacgt cgcccgggaa tgcttcacgt cctggcggac gacggagcag
     7261 cagggagatc tgacggtaag caacagcctg tttagacagg tcatcgtaaa tgatcagcgc
     7321 atcttcaccg cggtcacgga agtattcgcc cattgcgcaa ccggcatacg gagccagata
     7381 ttgcagtgca gcggattcag acgcggttgc taccacaacg atggtgttag ccagtgcgcc
     7441 gtgctcttcc agtttacgta ccacgttaga aatggtggac gctttctggc cgatagcgac
     7501 atagatacat ttgataccgg aatcgcgctg gttgatgatg gcatcgatag ccagtgcggt
     7561 tttaccggtc tgacggtcac cgatgatcaa ttcacgctga ccacgaccaa ttgggatcat
     7621 ggagtcaacg gctttataac cggtctgtac cggctgatct acggactgac gttcgataac
     7681 gcccggagcg attgcttcta cagcagagaa gccgtcgtga tccagcggac ctttaccgtc
     7741 gattggtgca cccagagtgt taaccacacg gcccagcagg ccacggccaa ccggaacttc
     7801 caggatacgg ccagtacact taactttcat gccttcggca aggtcagcgt acggacccat
     7861 aacaaccgca cctacagagt cgcgctcgag gttcagtgcg atagcgtaac ggttacccgg
     7921 cagggagatc atttcaccct gcatacaatc ggccaggccg tgaatgcgga taacaccgtc
     7981 acttacagaa acaatagtac cttcgttgtg agcttcactc acaacattga actgagcaat
     8041 gcgctgcttg atcagttcgc tgatttcggt ggaattcagt tgcatgctcc agtcccctta
     8101 agactgcaag acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat caatgaccat
     8161 atcacccgct cggatgataa cgcctgccat tacagactta tcgattttgc aattcagctt
     8221 aactttgcgt gacagacgtt tttccatcgc agcagaaatt ttcgcgagct gttgttcact
     8281 cagtgcggca gcggaaatga cgtctacctc agcggtagcc tcactcacgg cacgaaggtg
     8341 aataaactgc tccagaacat ccgggagcgc gttaagacga ccattttcag ccattacccg
     8401 aatcaggttc tgaccgtttt cgtccagttg ctcaccacaa actgcgataa acgactcggc
     8461 gagcgtttct ggcgcaagcg cgccagagag aagctctgcc atttgttcgt ttttggttac
     8521 ctcggcggca aacgccagca tgtcctgcca gcgttctaca ctttggtgtt cgacggcaaa
     8581 gtcaaaagct gctttggcgt aggggcgagc taccgtaata aattcagaca tcagcccctc
     8641 cctccttaca gttcagcgac aagtttatcc acgatgtcgc tgttagcagc ttcatccacg
     8701 gaacgttcga tgatcttctc ggcgccagca acagccagga tagcaacttg cttacgcagc
     8761 tcttcacggg cacgtttacg ctcggcttca atttctgcct gcgcctgggc cacgatttta
     8821 gtacgttcct gttctgcctc agctttcgct tcgtccagaa tctgcgagcg gcgtttgttc
     8881 gcctgctcga tgattacctg ggcttccgct ttcgcttttt tcagctggtc ggtcgcgctg
     8941 gcctttgcaa ggtcaaggtc cttatgtgct cgttctgcgg aagcaaggcc gtcagcaatt
     9001 tctttttgac gtttttcgat ggctgccatt aatggcggcc atacgtactt catgcagaac
     9061 agaacgaaca ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag attcacagca
     9121 caatgcctct atttagttaa cgttctgata ttgctcttta aataaaagca acgcttacta
     9181 cgcgacagcg aacatcacgt acagacccag acctacagcg atcatcggga tagcatccac
     9241 cagacccata acgataaaga actgagtacg cagcagagga atcagatcag gttgacgcgc
     9301 tgcgccttcc aggaatttac ccccgaggat gccgataccg atcgcagcac cgattgccgc
     9361 cagacccatc atcacagcgg cagccatgta cagcagatcc atattcaggt tttccatgac
     9421 agtctccagt ttgtttcagt taaaacgtag tagtgttggt aaattaatgt tcttcagacg
     9481 ccatcgacag atagacgatc gtcagaacca tgaagatgaa ggcttgcagc gtaatgatca
     9541 ggatgtggaa aatggcccac ggcacattca ggatccactg tgaccaccac ggcaacagac
     9601 cagcaatcag aatgaaaatc agctcaccgg catacatgtt accgaacagt cgcaaaccaa
     9661 gtgaaactgg tttggacagc aggcttaccc cttcaaggat taagttgaca ggaatgaacg
     9721 cccagtgatt gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg cctttcattt
     9781 tgatgctgta gaacagaatc aggataaata cgcccaatgc catagacagc gttacgttca
     9841 cgtccgcaga cggaaccaca cgcagtgcag gcagacccag tacatgttca gcaatgtacg
     9901 gcagcaggtc gataggcagt aaatccatca ggttcatcag gaatacccag acgaagatcg
     9961 tcagggccag cggagcaatc agcttgcttt tgccatggta catgtctttc acgctaccat
    10021 taacaaagcc gatcaccagc tcaatcgcgg tctgaaactt acctggcaca ccgctggtcg
    10081 cctttttggc tacgctacgg aataaaacca ggaacaacag acccagcacc accgagaaga
    10141 acatggagtc aatattgatt gtccagaagg tggctggggg gttttgtgga tccaccagcg
    10201 agaatgtacg caggtccagc tgaaggttat tcaggtggtg tcctatgtaa tcctgcggcg
    10261 tcatattttc tgaagccatg atgcctttta ccctttgttg ttaattacag ccggtgccag
    10321 tatctgaacc accagcacca aaacccacgt aacgatcagc ggcaagaata ccgcctttaa
    10381 aaccgccaac gccaccacca gtaacaccaa catcgccaga actttgaaag cttcgccaaa
    10441 tgcgaatgtc caggccaccc ggcctttcgc tggtgtatgc gcctggtgac gccaggcaaa
    10501 tatcataaac aaaacgttag gcagaaagac tgccaggccc ccgcttattg cagagacgcc
    10561 ccagaagggg tctttgaggc tgaacagcaa tccacttgct atcaccacca gtaactgaac
    10621 gagcagaagc ttccgagcaa cgtttcgact cacgagcgac acagacatca cgtttttcac
    10681 tcctgctccc ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg cttagagtca
    10741 agcatcaaaa agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca ataagtagcc
    10801 aaaaggtgaa taaatgttta aatatttttc cagtgcatac aattgcgact tttctgctaa
    10861 ccctgttcga tcatgaaaaa ctgtaaataa cgcgtaaaca ctggtgataa agcgtgcttc
    10921 agatcacata ttgcgcatgt tcgcgcacag catatttatt tacttggcaa atgatgcctt
    10981 tgcaagttta tgatatttca gtctaaaaac agatactgtt ttaataaatg acatttacac
    11041 aacaaaaacc acccattgac atttttaata atgttttaac agccaatgat ggttcttagc
    11101 gccgattttt agcagactga tattttcact aatgacttat tttctgctta ccaaaaaaag
    11161 ccacgttatc ttgttgatgc aaaagagtga acgtggcgtt aaatgtaacc agttatatca
    11221 gtagaaaacc tggttgttgt taacagtcta accggtcaat tttttatgat ttttttgata
    11281 aaaattaaat tttatttgct ttaatcacca ccagatgacg ttcgccatcc aaggctggaa
    11341 cctgaagttt aaccactgat tcgacctgat attcttcggg caacaaagcg atttcatctt
    11401 ccggcatttg ccctttcagc gcgtagaaac ggccttgctc accaggaaga tggtggcacc
    11461 agctcaccat atcgttcaga gaggcaaaag cgcggctaat tacgccatca aatggcggct
    11521 ctgaaggaaa ctcttctacc ctgctctgta ctggttcaat attctccagt ttaagctcat
    11581 gttgcacctg acgaaggaaa cgcacgcgtt taccaaggct atccaacaga gtgaaatggg
    11641 cttcaggacg cacgatagag agtggaatgc ctggcagtcc tggtccggtg ccgacatcga
    11701 taaaccgttc accttgcaga tacggtgcca ccacaatgct atcgagaata tggcgtacca
    11761 gcatctcatt aggatcgcgg accgaagtca ggttgtacgc tttgttccat ttatgcagca
    11821 tattcacgta ggcaataagc tggtttttct ggtgatcggt aagcgaaata cctgcgtctt
    11881 tcagcagtaa ggagagtttg ttgagcacgg tgattacctg ttcttgatgc gttgcctggt
    11941 aagcgggtgc ttaccaggca tttttaatgc gttatgcgct acgacgcagc ataccctgtt
    12001 ttttcagcca caccagcaga atggagatgg ccgcaggcgt gacgccagaa atacgcgaag
    12061 cctggccgat agaggctggt ttgtgatcgt taagtttggc gatcacttcg ttagaaagac
    12121 cggatacctg gcggtaatcc agtgtcgcgg gtagcagggt gttctcgtta cgcagctgct
    12181 tttcgatctc atcttgctgg cgcgcgatat aaccttcgta tttaacctga atctcaacct
    12241 gttccgccgc ctgttcgtct gtcaacgcag gggcaaacgg cgtcagcgtg gttaattttt
    12301 cataagtcat ttccggacga cgcagcagat cttcaccact ggcttcacgg gaaagcggcg
    12361 cagtcaggtg agcattcact tcggctgcag cttccgccga cggggttacc caggtcgatt
    12421 tcagacgctg acgctcacgc tcgatattct caagtttctc gttaaagcgc gcccaacgtt
    12481 cgtcatccac caggcccagt tcacgaccga tttcagtcaa acgcagatcc gcattatctt
    12541 cgcgtagcat cagacgatat tctgcgcgcg aagtaaacat acgatacggt tctttggttc
    12601 ctaaagtgca caggtcatca actagtacgc cgagatacgc ctgagaacgt gccggagccc
    12661 aaccttcttt gtcagcagac agacgggcag cgttaagacc ggccagcaaa ccttgcgcag
    12721 cggcttcttc gtaaccggta gtgccgttaa tctgaccagc aaagaacagc ccctggataa
    12781 acttgctctc cagcgtcggt ttcaggtcgc gaggatcgaa gaagtcatac tcaatggcat
    12841 aacccggacg cacgatcttc gcgttttcca tcccctgcat agagcggacg atttgcatct
    12901 gcacatcgaa cggcaggctg gtggagatac cgttcggata aatttcatta gaggtcagtc
    12961 cttccggttc aaggaagatc tgatgctgat ttctgtcggc gaagcgcata actttgtctt
    13021 cgatcgacgg gcagtagcgt gggccgacac cttcgatcac ccctgcatac attgggctac
    13081 gatcgaggtt actgcggatc acatcatggg ttttctcgtt ggtatgagtg atataacacg
    13141 gcacctgctg gggatgctgg gacgcattgc ccataaacga gaataccggc attgggttat
    13201 cgccatgctg ttgcgccagc acactaaagt cgatagttcg agcatcaata cgcggtggtg
    13261 tcccggtttt cagacgacca acgcgcagcg gcagttcacg caaacggcga gaaagcggaa
    13321 tggacggcgg atcaccagca cggccaccgc tgtaattatc cagaccgata tgaattttac
    13381 cgtcgaggaa cgtcccaacg gtgagcacga cggctttggc acggaacttc agtcccattt
    13441 gggtaacagc accgaccacg cgatcgtttt cgacaataag atcttcaacc gcctgctgga
    13501 agatcatcag gttcggttgg ttctccagcg ccgtacgtac cgcctgacgg tagagcacac
    13561 gatccgcctg agctcgggta gcgcgaaccg ccggtccttt gcttgcgttt agtatcctaa
    13621 actggatacc cgcttgatcg atcgctttcg ccatcagacc gccgagtgca tccacttctt
    13681 ttaccagatg tcccttccca ataccgccga tcgccgggtt gcagctcatc tgccccagag
    13741 tgtcgatatt gtgtgtcaaa agcagagtct gttgacccat acgcgccgcg gccatcgcgg
    13801 cctcggtgcc tgcatgaccc ccgccaatga tgatgacgtc aaaaggatcc ggataaaaca
    13861 tggtgattgc ctcgcataac gcggtatgaa aatggattga agcccgggcc gtggattcta
    13921 ctcaactttg tcggcttgag aaagacctgg gatcctgggt attaaaaaga agatctattt
    13981 atttagagat ctgttctatt gtgatctctt attaggatcg cactccctgt ggataacaag
    14041 gatccggctt ttaagatcaa caaccgggaa aggatcatta actgtgaatg atcggtgatc
    14101 ctggaccgta taagctggga tcagaatgag gggttataca caactcaaaa actgaacaac
    14161 cgttgttcct tggataacta ccggttgatc caagcttcct gacagagtta tccacagtag
    14221 atcgcacgat ctgtatactt atttgagtaa attaatccac gatcccagcc attcttctgc
    14281 cggatcttcc ggaatgtcgt gatcaagaat gttgatcttc agtgtttcgc ctgtctgttt
    14341 tgcaccggaa tttttgagtt ctgcctcgag tttatcgata gccccacaaa aggtgtcata
    14401 ttcacgacta ccaataccga ttgcgccaaa gcggaccgca gaaagatcgg gcttctgttc
    14461 ctgcaatgct tcatagaaag gagaaaggtt gtccggaata tctccggcac cgtgggtgga
    14521 gctgataatc agccagatcc ccgaggcaga taaatcttcc aacagcggac cgtgcagcgt
    14581 ttcggtggta aaacccgcct cttccagctt ttcagccagg tgttctgcta catattcggc
    14641 accgccgagg gtgctgccgc tgataagagt gatatctgcc ataaaccgcc acctttatta
    14701 agagtggcgt attgtacgct gtgaacgcgt tgggatctac ctgtggaaaa gtatgggatt
    14761 aaaaaagccg attatggctt gatggtacgc atgatcgggt tttgcaggac gatcaatgtc
    14821 tcggtggact gaatttcatc aattgtttgg atcttgttga taagtacatg ctggagagcg
    14881 tcgatcgaac ggcacatcac ttttataaag atgctgtagt ggccggttgt gtaataggct
    14941 tcagtgactt catcaaggct ttccagcttt gccagcgcgg aagggtagtc tttggcgctc
    15001 tttaatataa tgccgataaa gcagcctacg tcataaccga gctgcttcgg gctgacatca
    15061 atacgcgccc cggtaatgat ccccgcctgc ttcattttct ctactcgaac gtgaatcgtc
    15121 cccggactga cgccaaattg tttcgccagt tcggcgtaag cggtgcgcgc attgcccatt
    15181 aatgcttcca ggatgccacg gtccagattg tcgatcagat aattttccat aggattttct
    15241 tatgcagatt gatgattcat tctattttag ccttcttttt taatgaatca aaagtgtgtt
    15301 aggcttttta ttgaatgatt attgcatgtg tgtcggtttt tgttgcttaa tcataagcaa
    15361 caggacgcag gagtataaaa aatgaaaacc gcttacattg ccaaacaacg ccaaattagc
    15421 ttcgtgaaat ctcacttttc tcgccaactg gaagaacgtc tggggctgat cgaagtccag
    15481 gcgccgattc ttagccgtgt gggggatggc acgcaggata acttgtcggg ctgtgaaaaa
    15541 gcggtgcagg taaaagtgaa agctctgcct gatgcccagt tcgaagtggt tcattcactg
    15601 gcgaagtgga aacgtcagac cttagggcaa cacgacttca gcgcgggcga agggctgtac
    15661 acgcacatga aagcccttcg ccccgatgaa gagcgtcttt ctccgttgca ctcggtctat
    15721 gttgaccagt gggactggga acgcgtaatg ggcgacggtg agcgtcaatt ctcgactctg
    15781 aaaagcacgg tagaggcgat ctgggcggga attaaagcaa ccgaagctgc agttagcgaa
    15841 gagtttggtt tggcaccgtt cctgccggat cagatccact tcgtacacag ccaggagtta
    15901 ctgtctcgtt atccggatct cgatgccaaa gggcgtgagc gggcgattgc gaaagatctt
    15961 ggcgcggtat tcctcgtcgg gattggcggc aagctgagtg atggtcatcg tcacgacgtg
    16021 cgcgcaccgg attatgatga ctggagcacc ccgtcagagc tgggccatgc gggtctgaac
    16081 ggcgatattc tggtatggaa ccctgtactg gaagatgcgt ttgagctttc ttccatgggg
    16141 atccgcgtgg atgccgacac gttgaagcat cagttggcgc tgaccggtga tgaagatcgt
    16201 ctgcagctgg aatggcatca ggcgctgctg cgcggtgaaa tgccgcagac catcggcggc
    16261 ggtatcggcc agtctcgttt gaccatgctg ctgctgcaac tgccgcatat cggccaggtt
    16321 cagtgtggag tatggccagc tgctgttcgc gagagcgtcc cttctctgct gtaataattt
    16381 atcgccgcca gcgtctgagc aggcggcttc gcatcccggt atcaaagcgc cagatatgat
    16441 cgaaaatgcg catgatgccg ggtttgccgt gtgccgacat cgccacggca tgaaagcgat
    16501 gctgatgtac ccgctgcagc tctttcactt tactcgtcac gtcgtcaggc aaccgctgag
    16561 cgataaaatc agaaatcacc accgcatcgg catcaaacca ttccctgctt tgcaagcgtt
    16621 ccataatggc gcgaaaacaa ctggcaagat cggtgccgcc acgaaactgc tggcttaaaa
    16681 aacggattgc ttgttcgatg ccttgtgggc ctgaaagctc ataacggacg atctcggtgg
    16741 aaaatagcat aatatagcag cgccggtttt cagcgagagc aatgcgcatc aaggccaggc
    16801 agaacgcttt cgcacactgt tcattaaagc cgcccattga gccggaagta tccacacaga
    16861 caataaacgg cccgcgcggc tgttcatcgt aatctttatg taccaccgga cgttcgatca
    16921 ctttttcacg ccacgactca ccgtgcaggc gataggtgag caactgtttt tccaccagcc
    16981 gacggtaaaa ctcatactcc agttccgtta tccctagtgt cgccagttct ggcggcagaa
    17041 gacgtaaaat atcatcgctt tgttgcagac catcaacctg ctcaggaacc gtcgccggtt
    17101 cgcgcaccat ggtgcggaag gtttccatct gcgcatcgtt gcgcggtatt gatttggctt
    17161 cccgagaacg ccccagctgc tctgccaggc gtttcagttc cggctgttcg ttaagaaatt
    17221 caccgtattt cacaatcaac tgatagtcgc cacgtttaag ctgaccggcg ctcatatccc
    17281 acagacgacc agctgcagta ttgttatctg cgagaatcgg ttcaagttgt ccgctcagcg
    17341 tcatgcgttc ctgaacttca ctcaacagtt gttcgcgttc ttcttctaat agctgttgat
    17401 ttaacgtcgt tgcttgcacg atcagactta aacgccaacg ctggagaaaa agcgtgtgta
    17461 acgctgaagt gatcgtgctg ttagcatcaa ccaactgtcg ggcttgttct gcccatggag
    17521 aattcagacg atgcagtaag tccaggatct gtggtagctg cacaataaac tgtggcgtgg
    17581 agaggagctg gctttgctga tagcacatca cctcttcggt gagttccggc gggactcggg
    17641 catctttcag ccgactgcgc agcgcttcac gccagcgggg aacatcatca gtgattgccg
    17701 ccttcaggcg tgggaatttt tcaaagaaga ccgccagctg cggtgaggcc agcagtgcga
    17761 tgatcatctc ttcgatcaat ccctcttcgc tgacggccag catcacatta agcgtatcca
    17821 gcgttagcat tgttgtgcct ggcgaatctg tgcaccgaca tcctgtaggc tggcttcaat
    17881 gcgacctaac cagtcgccag ggataaacag acatttttgc tgttcgctga ataaagcgtg
    17941 ttgcttgcgc cagtcgcttt caagctcttc cagttgttgt tttatttcac caggcagacc
    18001 ttcagccgat gaaccgggaa gtgccagcgt actgccttgt aaacttacat cgcgaacgac
    18061 aagatgttgg gcgctatcaa cttccagatt cagtttctgg gcaaagccga taccgttcag
    18121 tttaccgcga atttcaccac ctttgctcag ccactgttcc agcgcgctac gctcaaagga
    18181 gatatgaacc acttccatat catgcagttt taacggtttt tgcagcagta gagtcagagt
    18241 ggaagcagta acgttaacag gaagttgata ctgctgacga cggctgaaaa tgccgcccag
    18301 acgaattacc gttaaggctg ttttatcgct ttgttgctgc tggagttgca ggtgacgttg
    18361 cacaatcgcg ccaaggcgag tcaacatccc ttgctgttgc caggcgtgac cggtcatcaa
    18421 tacatcaatt tgttgttgta tcaaattcag gctttgcgcg tcataccaca ggcaatcttt
    18481 cagcaaaatg agatctaccg gggcaacagc actgcgaccg ctaaaaaagg cgctggcctg
    18541 caataatcgg atcgcttttt tccagcgacg atccgagaca taaggcgcat ccggtaattt
    18601 atccagttgc tggcgcagca taaaaatcag ctcaaataca tgatcgggca gcgtaatttc
    18661 accaatctct ttctgccagc gttcatattc ttcatctgtg acctgcaagg catcaggaac
    18721 cggattgtcg ttttcatcct gttgactggt cagcatggag cggaaattcg ctttatcctg
    18781 cactttatct aaccacagac gaatcagcat gcggtcatat aacgcttcca gactgctgtc
    18841 tgcttccggc agctcgttgg aggccgccac cagcagacgc atcgggattt tttctacgtg
    18901 tgcaccgttg cggaactggc gctcgttaat ggcggtgagc aaggtattaa gaattgccgg
    18961 gcccgctttc cagatctcat ccagaaagac aatttctgct tccggcaggt aaccgctggt
    19021 taaacgttca tagcgccctt catcttttag cgcctgaata gaaaggggac caaaaacttc
    19081 ttccggcgtg gagaagcggg tcatcagata ttcaaacgcg cgggcattct gaaaggcgaa
    19141 ttttaagcgc cgggcgatca aacttttggc aatacctggc gggccaagga ggaacacact
    19201 ttcaccactt aatgccgcta ataaacacaa gcggatggcg tggctacgtt cataaagccc
    19261 cttttccaac gaactgctca ggcgggaaat tctttccgct aataaatgag ggtgagccat
    19321 aatgaagtgg cgtcctttcg tcaaaagttc tgcgtaaatt gcgagtatag acgtttcttg
    19381 ctggtggcta aaatagtctc aaaggggggg tatttttctt tgagccaggt taatgtggcc
    19441 gcatttagga gtacgatttt gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa
    19501 gggactaagc acacatttca tatttcaacg aaagactagt ctatgagcac tgataataag
    19561 caatcattgc ccgcgattac cctcgcggcg attggagttg tctacggcga tattggtacc
    19621 agcccgttat atacacttcg tgaatgtttg tccggccagt ttggttttgg cgttgaacgc
    19681 gataccgtgt ttggcttttt atcgctgatc ttctggctgc taatctttgt ggtttccatt
    19741 aaatatctca ccttcgtgat gcgggcagat aacgccggtg agggggggat cctgacgttg
    19801 atgtcgcttg ccgggcgtaa tacgtcggcg cgaaccacat caatgctggt gattatgggg
    19861 ctaatcggcg gcagcttttt ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg
    19921 tcagccattg aaggtctgga aatcgtcgcc ccgcagctgg atacctggat agttcccctc
    19981 tcaattatcg ttctcacttt attatttatg attcaaaaac atggcacggc tatggtcggt
    20041 aagctgtttg cgccgatcat gctgacctgg tttttgattc tggcaggact ggggttacgt
    20101 agcattattg ctaacccgga agtgctgcat gcgctgaatc caatgtgggc ggtgcatttc
    20161 ttccttgaat acaaaacggt ttcttttatt gcattagggg cagtggtgtt gtcgattacg
    20221 ggggtcgagg cgctgtatgc tgatatgggg cactttggta agttccctat tcgcctggcg
    20281 tggttcaccg tcgtattacc ttccttgacc cttaattact tcggtcaggg agcgctgttg
    20341 ttaaagaacc cggaagcgat taagaacccg ttcttcctgt tggcaccgga ctgggcgctg
    20401 atcccgctgc tgatcatcgc cgcactggcg acggtaattg cctcgcaggc ggttatctct
    20461 ggcgtcttct cattgacgcg tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt
    20521 attcacacct ccgaaatgga gtcagggcaa atctatattc cgtttgtgaa ctggatgctc
    20581 tatgtcgcgg tcgtgattgt gattgtcagc tttgagcact ccagcaacct ggcggcggcg
    20641 tacgggattg cggtgaccgg aaccatggtg ctgacgtcta ttctctcgac taccgtggca
    20701 cgtcagaact ggcactggaa taagtatttt gttgcgctga tcctgattgc tttcctttgt
    20761 gtcgatattc cattgttcac cgctaacctc gataaactgc tctccggcgg ctggttgcca
    20821 ttgagcctcg gtactgtgat gtttatcgtg atgaccacct ggaagagcga gcgtttccgc
    20881 ttgctgcggc ggatgcatga acatggtaac tctctggaag cgatgattgc ttcgctggag
    20941 aaatcaccgc ctgttcgcgt gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc
    21001 attccctttg cgctgatgca taaccttaaa cataacaagg tattgcatga gcgggtgatt
    21061 ctgttaactc tgcgtaccga agacgcgcca tatgtccata atgtccgtcg ggtacagatt
    21121 gaacaactgt cgcccacttt ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca
    21181 aacgtggaag aagttttcca ccgctgcggt ctggaaggat taagttgccg gatgatggaa
    21241 acctccttct ttatgtcgca tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt
    21301 ctgcgcggca agctgtactt gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt
    21361 gaaattccgc caaacagggt tatcgaactg ggtactcagg tcgaaatcta acgccagacg
    21421 cctcctttct ccataagggg gcgtttttgt tttcatggtt aatcaccatg taaaacgttt
    21481 cgaggttgat cacatttccg taacgtcacg atggttttcc caactcagtc aggattaaac
    21541 tgcgggtcag cgaaacgttt cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa
    21601 ttctgatatt tcatcggtga tctcccgtct gggacatacc gatacgctgg tggtgtgtga
    21661 tgctggttta cctatcccca aaagtacaac gcgtatcgat atggcattaa cccagggtgt
    21721 accttctttt atgcaggtgc tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat
    21781 tatcgcggaa gagatcaaac aacataatcc gcaactccac gaaacgttgc tcactcacct
    21841 tgagcagctg caaaaacacc agggaaatac cattgaaatt cgttacacca cgcatgaaca
    21901 attcaaacaa caaaccgcag aaagtcaggc ggtaattcgc agcggagaat gttctccgta
    21961 tgcgaatatc attctctgtg ctggcgtgac gttctgaggc cgtcatggaa gcattacttc
    22021 agcttaaagg catcgataaa gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa
    22081 atgtctatcc gggccgcgtg atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca
    22141 tgatgaaagt gcttactggt atctatgctc gcgatgccgg cacgctttta tggctgggga
    22201 aagaaacgac atttaccggg ccgaaatctt cccaggaagc cgggattggg attatccatc
    22261 aggaactgaa cctgatcccg cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt
    22321 ttgttaatcg ctttggcaaa attgactgga aaaccatgta tgccgaagcg gataaattgc
    22381 tggctaaact taacctgcgc tttaaaagcg acaagctggt gggcgatctt tccatcggtg
    22441 accagcaaat ggttgaaatc gccaaagtgc tgagctttga gtcgaaagtc atcattatgg
    22501 atgaaccgac cgatgcgctg accgataccg aaaccgaatc cctgttccgc gtcatccgcg
    22561 aactaaaatc gcaaggccgc ggtattgtct atatctccca ccgcatgaaa gaaatcttcg
    22621 agatttgcga tgacgttacc gtttttcgtg acgggcaatt tattgctgag cgcgaagtgg
    22681 catcactgac cgaagattcg ctgattgaga tgatggtggg ccgcaagctg gaagatcaat
    22741 atccacacct ggacaaagcg ccgggagata tccgcctgaa agtcgataat ctctgcggac
    22801 ctggcgttaa cgatgtctct tttactttac gcaaaggcga aattcttggc gtctctggtt
    22861 tgatgggcgc aggtcgtacc gaactgatga aagtgctcta cggcgcacta ccgcgcacca
    22921 gcggttacgt caccctggat gggcatgaag tcgttacccg ttcaccgcag gatggcctgg
    22981 caaacggcat tgtgtatatc tccgaagacc gtaaacgtga cggtttagtg ttgggcatgt
    23041 cagtaaaaga gaacatgtcg ctgacagcgc tgcgctactt cagccgcgct ggcggcagtt
    23101 tgaagcatgc cgatgaacag caggctgtga gtgatttcat tcgtctgttt aatgtgaaaa
    23161 caccgtcgat ggaacaggca attggtctgc tttccggtgg caatcagcaa aaagtggcga
    23221 ttgcccgcgg tctgatgaca cgccccaaag tgttgatcct tgatgaacct acccgtggcg
    23281 tagatgtcgg cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct
    23341 tgagcatcat tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca
    23401 tcgtcatgca tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag
    23461 tgttaatggc tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca
    23521 acccagactg tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg
    23581 cttatcgctc tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc
    23641 atcaataact tattcaatat tctccagcaa acctcggtga acgccattat ggcggtcggg
    23701 atgacgctgg tgatcctgac gtcgggcatc gacttgtctg taggttctct gttggcgcta
    23761 accggcgcag ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct
    23821 gccgctctcg cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt
    23881 cgcgttcagg cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg
    23941 gtttatacca acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc
    24001 tggtttggta ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc
    24061 ttcctcgcgg cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg
    24121 ggcggcaacg aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc
    24181 gtctattctc tttgtggtct gctggcatcg ctggccggga tcattgaagt ggcgcgtctc
    24241 tcctccgcac aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt
    24301 ctgggcggta cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca
    24361 ttaattcttg gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag
    24421 atgatcgtca aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa
    24481 taacgactac aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct
    24541 gttgcgctaa gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg
    24601 gtctccacgc ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg
    24661 gataagcttg gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg
    24721 gcgaacgtgc aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgaccgac
    24781 tccgacgcag taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact
    24841 cttgaccgcc aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg
    24901 ggtggcaaaa tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc
    24961 gagctgcaag gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag
    25021 gccgttgctg ctcacaagtt taatgttctt gccagccagc cagcagattt tgatcgcact
    25081 aaaggtttga acgtaatgca gaacctgttg accgctcatc cggatgttca ggctgtattc
    25141 gcgcagaatg atgaaatggc gctgggcgcg ctgcgcgcac tgcaaactgc cggtaaatcg
    25201 gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt gaatgatggc
    25261 aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg cgtcgaaacc
    25321 gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga tctgaaactg
    25381 gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa atacgtcatc
    25441 gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac acggtggcgc
    25501 attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt ggcagcatta
    25561 atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc gtaaccggta
    25621 accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta
    25681 gcggtgcgaa tatcgcgttt attgcctgta cgggtgatga cagcattggt gagagcgttc
    25741 gccagcagct cgccactgat aacattgata ttactccggt cagcgtgatc aaaggcgaat
    25801 caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg
    25861 ccggcgctaa tgctgccctt tccccggcgc tggtggaagc gcaacgtgag cgtattgcca
    25921 acgcgtcagc attattaatg cagctggaat caccactcga aagtgtgatg gcagcggcga
    25981 aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct cgcgaacttc
    26041 ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc
    26101 tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg
    26161 aaaaaggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg gctagcgtga
    26221 atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc attgctgccg
    26281 gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca ttgccagagg
    26341 cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc gcacaacctt
    26401 ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga cgcttggcta
    26461 caatgaaaga tgttgcccgc ctggcgggcg tttctacctc aacagtttct cacgttatca
    26521 ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg attaaagaac
    26581 tcaattacgc gccatcagcc ctggcgcgta gcctcaaact caatcaaaca cataccattg
    26641 gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt ggcgttgaac
    26701 gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaagga gatgaacagc
    26761 ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggcttg ctgttactgt
    26821 gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg
    26881 tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat aactcgttgc
    26941 tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt atcgcctgta
    27001 ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat cgggcggcga
    27061 tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat tttgaattta
    27121 acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct caggccgtct
    27181 ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag gcagagttac
    27241 aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca agctttatga
    27301 cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg gcgattgatg
    27361 tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa cttactccga
    27421 ttctgatgga acgcggttcg gcttagattt acgctgtctt ttgatcaaat tattaccatc
    27481 ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc ccatcgtgat
    27541 aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat aaacgcgaag
    27601 gacggccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga ccgccagaac
    27661 actgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg tattcatcgc
    27721 ggtaaattgc gtcgacatag ccatccctaa tataaacaac ggcaagatca gcatccatat
    27781 cgccattacc ggtgattgca aagagaactg agcgatcatt agcccaataa tcaccgtgat
    27841 ccccactaac gtatggcgat agcccagacg acgtaagact tgggtaacca tcgattttgc
    27901 aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg cctgataacc
    27961 aaatcctacc tgtaacatca atggcataag gaacggtaca caaccggtcc ccagacgggt
    28021 tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat ctaatgaaat
    28081 taatgggttt ggcgtgtgtc gcgcatggag aatatagaga agcagtaacc cgatgctggt
    28141 gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata gctctattcc
    28201 gcttgagaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat cgaatctgcg
    28261 tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg caatacctat
    28321 ggggatattg attaaaaata tccagtgcca ggttgcccag gtgaccagca cgccgccaag
    28381 aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca atactggaag
    28441 aagttcatta cgaggataag cgcgcagtaa ggccagccga gcaacaggca tcatcattgc
    28501 gccgcctatc ccctgaataa cccggaagac aaccagctgt ggtagcgaat tagaaagtgc
    28561 gcaggccaga gaacccaatg tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc
    28621 gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca gcgtataact
    28681 gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga ttgcgggtaa
    28741 ggcggtattc agaatagtgg catcaagtgc ctgcatgaag aaggccatcg ccgcgatcca
    28801 cggcaaaccc gccatactgc gcttcttttt atcgctcatt caatgtccta ttatcgggtt
    28861 gtcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc
    28921 tttggacaat cgcatcgaca atcgcctggt gcagatccag ctttatcact gtgtcgctgg
    28981 taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa
    29041 acggattggc gctcatttca tagatatgct catgccaggc catatcgact tcgatccagc
    29101 gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta ttgagatgcg
    29161 ccttctgttc cgcggtgcca accgttgcag ccagtaggca agcttgcggc tccagacaga
    29221 tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc atccaccagg
    29281 taagcaattc ctgatcaaga aaattccagt ttgattgtgg catgacccgg gtaccaattc
    29341 gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg
    29401 tacgactcac tccaaattgc tcgcccagct caatctcacc aggcaaaatg gtgccgggtt
    29461 catattcacc ttttaagatg cgttgcgcca gcttctcagc cagaacatac gaaaggtttt
    29521 tctgtgcagc taactgttgt gcgcttaaag gcattactaa tcttcctttt tctttttatt
    29581 cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa attgcttgtt
    29641 ttatggcaca ttaacggggc ttttgct
//
LOCUS       DAZFKM010000055        29626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFKM010000055 DAZFKM010000000
VERSION     DAZFKM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHKAY_002470"
     CDS             complement(131..778)
                     /gene="gloC"
                     /locus_tag="ACHKAY_002470"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO5701137.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(805..1353)
                     /locus_tag="ACHKAY_002471"
     CDS             complement(805..1353)
                     /locus_tag="ACHKAY_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO5701138.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHKAY_002472"
     CDS             complement(1534..3381)
                     /gene="ldtD"
                     /locus_tag="ACHKAY_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5701139.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHKAY_002473"
     CDS             complement(3642..8102)
                     /gene="mukB"
                     /locus_tag="ACHKAY_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO5701140.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTNCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHKAY_002474"
     CDS             complement(8102..8806)
                     /gene="mukE"
                     /locus_tag="ACHKAY_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO5701141.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHKAY_002475"
     CDS             complement(8787..10109)
                     /gene="mukF"
                     /locus_tag="ACHKAY_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO5701142.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSRTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHKAY_002476"
     CDS             complement(10106..10891)
                     /gene="cmoM"
                     /locus_tag="ACHKAY_002476"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO5701143.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11027..11806
                     /gene="elyC"
                     /locus_tag="ACHKAY_002477"
     CDS             11027..11806
                     /gene="elyC"
                     /locus_tag="ACHKAY_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO5701144.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11783..12676)
                     /locus_tag="ACHKAY_002478"
     CDS             complement(11783..12676)
                     /locus_tag="ACHKAY_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO5701145.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHKAY_002479"
     CDS             complement(12830..13576)
                     /gene="kdsB"
                     /locus_tag="ACHKAY_002479"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO5701146.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHKAY_002480"
     CDS             complement(13573..13755)
                     /gene="ycaR"
                     /locus_tag="ACHKAY_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO5701147.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHKAY_002481"
     CDS             complement(13807..15039)
                     /gene="ycaQ"
                     /locus_tag="ACHKAY_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO5701148.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHKAY_002482"
     CDS             complement(15076..16062)
                     /gene="lpxK"
                     /locus_tag="ACHKAY_002482"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO5701149.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHKAY_002483"
     CDS             complement(16059..17807)
                     /gene="msbA"
                     /locus_tag="ACHKAY_002483"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO5701150.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17844..20108)
                     /locus_tag="ACHKAY_002484"
     CDS             complement(17844..20108)
                     /locus_tag="ACHKAY_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO5701151.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHKAY_002485"
     CDS             complement(20315..20599)
                     /gene="ihfB"
                     /locus_tag="ACHKAY_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO5701152.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHKAY_002486"
     CDS             complement(20759..22432)
                     /gene="rpsA"
                     /locus_tag="ACHKAY_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO5701153.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHKAY_002487"
     CDS             complement(22543..23226)
                     /gene="cmk"
                     /locus_tag="ACHKAY_002487"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO5701154.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHKAY_002488"
     CDS             complement(23399..24163)
                     /gene="ycaL"
                     /locus_tag="ACHKAY_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO5701155.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHKAY_002489"
     CDS             complement(24332..25615)
                     /gene="aroA"
                     /locus_tag="ACHKAY_002489"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO5701156.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHKAY_002490"
     CDS             complement(25686..26774)
                     /gene="serC"
                     /locus_tag="ACHKAY_002490"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO5701157.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26973..27665)
                     /locus_tag="ACHKAY_002491"
     CDS             complement(26973..27665)
                     /locus_tag="ACHKAY_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO5701158.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHKAY_002492"
     CDS             27795..29555
                     /gene="ycaO"
                     /locus_tag="ACHKAY_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO5701159.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7194 a         8057 c         7191 g         7184 t
ORIGIN      
        1 gtcccctgca atattttgaa tttgcacgat tttgtaggcc ggataaggcg ctcgtgccgc
       61 atccggcata aacaaagcgc actttgccag aaatccaggc ccgcattaaa agcgggcttt
      121 tttatgtgcc ttaccagacg ggcatttcgt cttgcaggaa gggattatgc aggcgttcat
      181 aaccaagtgt ggataatggt ccgtgacccg gaataaatgt cacgtcatcc cccagtggca
      241 gcaatttatc tttgattgaa gaaatcagtt gattatgatc gccacgcggg aagtcactgc
      301 gccctactcc gcctttgaaa ataacatcgc cagaaatcag cagctttgcc cgatcatcaa
      361 aaaacacgac atgacccggc gtatgcccag ggcaatgtaa cacctgtaaa gtcacattcc
      421 ctatgctgat ggtatcgcct tcgttcagcc aacgatctgg cgtcagcggc tggcactctt
      481 ccagaccaaa catacgactt tgcgcaggca agccttgcag ccagaactca tcttcttttt
      541 ccgggccaaa aaccggcacg ccgtaatgtt gcgccagttc cgccgctgcg ccaacgtggt
      601 ccagatgacc atgcgtcagc aggatctgca tcagtgtcag gccgctgtca tcaacttcct
      661 gtttgatttt ttccgcatcg ccgccaggat cgaccagtgc ggccagacgg gtttgttcac
      721 accagattaa tgaacagttc tgggagaatg cggtgaccgg aataatacga tagttcatac
      781 tgcccctgtt tcgttaagca attactacca gtgccgtgct ggcccggtat caatatgcac
      841 aaagttacta cgtggataat atcctacacc acctgcgcgc atagataacg cggctttgcg
      901 aatattgctt aacgcgatac cttcaatatg gaaatccatc gcctggcctt tagtgtgata
      961 gcttttcttc gctactccac ggctgcgggc gcgtagttca ttgttggtat caatagaacg
     1021 ataaccggaa atgagttgca ccggtttgcg cgtgcctaac aacccttgca ggcgatacaa
     1081 ctggtcgaat aatcctgggt cgatggactt tattttgttc gcgcggtaat cgcggaaaaa
     1141 atggttaagt tttgccaatt cttcctgaat atagcctctg ccatcgaaaa actccgcttt
     1201 gattgactct ccggtatgaa gattattgag tgtcaaaatg cgcgggcgtg gggtagagag
     1261 tgttgcaaac gcaggggtcg gcaggatggc ggcaccgagt gcaacgccac caagcgccag
     1321 caatttgcgg cgattagcgt cgaatttgtc catgataatc aagtctacag gtcaatgtta
     1381 tcgtttatat gcacttctgg cgcacaagag gcaccttaac cgcccgtaaa gcagacgtca
     1441 agatgactca accccagcag tgacgggggc tgcagagaat cgcccccaat catgctaatt
     1501 attacgacaa ctgatttccc cgaactactt catttacctg attaattgtt ccgctttcga
     1561 tacgatttgc gagctggatc gcgcaggcag atcataattg taaatatctg tacgatactg
     1621 ggtacgacca tctgcaccaa caaaggccgt caggtagtag agattcaccg gaatcgactg
     1681 ccgaatattg acgtaacgtg tatcaccctg cttcagcgca tcagaaatac gtttgtcatt
     1741 ccagcctgca tcctgcaaca gcatattcgc cagatcggaa gctttattca ctcgtacaca
     1801 gcctgagctc aatgcgcgtg tatcacgctt gaacagattg tggttcggcg tgtcatgcaa
     1861 ataaatggcc tctgaactcg gcatattgaa tttatagcgc cccagcgagt tccgtgggcc
     1921 tggagcctgc tggaagcgga acggtaaatt cgaggccgtg attgtagacc agtcaacctg
     1981 ccatgggtca atcgcttctc tgctgttcca gccgcgcatc accgtatagc catggctttc
     2041 gagatatccc ggatcgttgc gcacttttgg cagaatatct ttgcgtgcca gagttggagg
     2101 tacgttccac ggcgggttta ccactacgtt gttaagggca ctgctcatca tcggcgtttt
     2161 gcgatcgggg cgaccgacaa tgactcgcga atccagcacc tgattgccgt tctgatagta
     2221 gaccagcgaa taggccggaa tgttaaccat gatcccggta gaaagctctg ttggcagcaa
     2281 gcgcaatcgc tggatgttga gagccaacac gccagcacgc tgggcgggcg ttacgtttaa
     2341 ccagtcacgt gttgccgggc caatagcacc atctgccccc aatccttgcc atgcctgaaa
     2401 acgtttaacg gcttccacca gttcattatc gtaggcggca cgaacggcag gcgcaggttt
     2461 actacgagac gtcgtttgct tgtccatcgg cttagtttct gctgtttcaa cagtaacagc
     2521 ggatgggctg actaccgcgt cagttggcgt gtcatcgcca ggtagagtaa ttttcggccc
     2581 cccgtccaac atgcctgtgc gttgcaatat ttcgcgcaac gccggtacgt cgttactcca
     2641 ctgccctggg cgcaacgttg ctttgccggt cagttggggc cacggtttgg tgtcactgag
     2701 taaggccagt aacgattcat gcatcgccgc atattgcgga tgctgcggtg ccagtcctgc
     2761 aacaaacgta ggcaattgac ctttatccag cgccagctgc cattggttaa tcaccgagag
     2821 cggcggcgtt gcaagtgcat aaggtttact gctatatagc cagcgagtgc ctttgaccgg
     2881 aatatttgca atgaaatgga gatagcccat catcgcatca gagagcacta cgtcgcgtgc
     2941 catcccgtta acaccaggat cggtcagtaa ctctacccat ttattaaact gcggctggaa
     3001 accggcaatc gccacctctg ccagctgttg ctggaaggct ttaacagcat cacggttttc
     3061 ccacatgggt tgcatatcgc gtgcggcata caacagttga agctggttaa gataaaccgg
     3121 cttgtaacct gcgggtaatt gagattcgat ttgcgtgcgg gctttttctg ccgcaccttc
     3181 aggcaatggc tggatccccg ccattattgc cgtggcttgc gcctgcggca gtgcctcgcc
     3241 ctgttcactg acagccaccg ggctgtcgcc agggattact tcaggctcat cggcctgcgc
     3301 attgaacagt ggagcgaatg ttacggccag gcacaaactg attgccgaca gccgacgacc
     3361 acacatcata ttaagcaaca tcccttgccc cctgttttta tcatactctg aatagccagg
     3421 ctgttatttt acgacttgcc tgtacaacgc caatactcac gaaagtatga cgagcaagcc
     3481 tgttactcat caggcttatc ttcagtatat aaacgccgat acgtttttac ataacctaat
     3541 gtaaagaagt ttaaagaaaa gcgcgccgta atgtgccact tttttagtgc ggaaacggag
     3601 ttttcggaaa aagaaaaggc ggcattgctg ccgccttaat tttaactcgc ctgagaaggc
     3661 gcttcgtcac ttcctggaag cgtttcaggg agttgcggcg caaatcctcg caggccgacg
     3721 acgtgaacgt gttcggtatt ctggaagact ttacgcacca gtttataggt ggtgcctttc
     3781 tccgggctga tattttccgg cgctgcgatg atgagctgca tttgcaaacg ctcacacaat
     3841 tcaaacagcg tggcgataga acgagcatcc agtcgcgctg cttcatcgag gaacagcagg
     3901 cggcaaggag agatatcttt accgcgcagg cggcgagatt catcttccca gctttgtacc
     3961 accatcacca gaatcgacat accggtacca atcgcctcac cggtcgacaa tgcaccagac
     4021 tctgcgcgca accaaccatc ggaaccacgg ttaacctcaa cttccatttc cagatagttg
     4081 cggtaatcca gcagttcttc gccaatggtc tgcggcgtgc gctgtcccat atcaatctgc
     4141 ggattaagac gctgatacaa tttcgccaac gcttccgaga aggtcagacg gttgctgtta
     4201 aacagatcct gatgctgctc gtgctgttca gagagcacat ccagcagcat ggcgtgcgtt
     4261 tcacgcacgt tcacgttgag gcgcacgctg ttcacctgac caaacgatac gttctgcaac
     4321 ccctggttga gcatacggat acggttctgc tcgcgctgaa tggttttgcg aatgatgttc
     4381 gccacgctgc gggaactgat cgccagtttc tgttcacggg aggttaattc ttcggtcaga
     4441 cggctaagtt cgatctccat ctgttcgata gcttccaccg gatcatcggt acgaataata
     4501 tcctgacgaa tacgttcacg cagatgctga taaaccgcca cgaagaactg aattttacgc
     4561 tccggacgtt tcggatcttc cgacatgcgc agcacgtcgc gcagatgttc gttatccgcc
     4621 accgccagac gcagcgcacc caatgcctta tccgacatag agcgcaaatc atcagcggag
     4681 agataagcca gctcacgacg gtgtaagcgg cgctcaacgc cgttatcttt caccatgcgc
     4741 atcaccgcac accagcccgc tttggcggtc actacctgct cgcgcatctc aaagtaatcc
     4801 cgctccagct tgcgcagttt gcgggtcagg ttgtccatct ccgcttcgca gaaggtaagc
     4861 gctttttcca gttgattgcg gcgtgaacgg ttattgctca gttgcgcatg cagctcgtca
     4921 cggcgaatac gcgcccgctc ttctgccccg ctatcagcac gcacgccgat atcctgcaat
     4981 tcacgttgca gatcgttgag tagctctttt ttggtgtcgt aagaactttt cagcgaagcc
     5041 agcacctggt tgtactgact caactgcgct gcgtgaccac gcaacgcttc acgagcgcga
     5101 gtacgctccg cttccgcctg ttccagacgt tcacgcagct tttcgttgag atcgctatta
     5161 ccgctcagca tttctgccga gtcagaatag ctaaagtgcg cacgacgctg caccacttcc
     5221 gtcagggcaa acgcctgctg acgggcatcg cgctgcatct gctgagagta cgcgtaatct
     5281 tcttttagct gttcgaactg ttccgggtcg ctctgcaata ccgaaacgat cggttccagt
     5341 ttcgccagtt gattgccaaa ctgctgaaca aaacgcgcgg cttcctgggc ttcatccaga
     5401 cgttcgcgga tttcatcgac gcgatccgcc aggctgtcat cagccaacag gttgagacgc
     5461 ggcagaatgc ggttcagcgc cgtaacgcct tctttcgcct gctcaaactg aatacgctgc
     5521 tgctggttat cattttcatg attactcaac gcccgctcca gttcgacgcg acggctgttc
     5581 agttgacgga tttctgcttc cgggtcagac tcaaacgcaa ccgccagatg actgccgata
     5641 aagcggctga acgcctgatg cagacgctga gttttctgta catcaaagga gagcgtggcg
     5701 aagcgttcgg aaagcacttc acgctcggca tggaggcttt caatacggct ttcacgcgca
     5761 gcacgaccaa acagcggcac ttccgggaaa cgtgaataac gccactgacg atcggcgatt
     5821 ttcaccacta ccgctttttc cagctcatca acgctgaaca cgctgtcatc gaatgactgc
     5881 ggatctcctt cgatcagata gagatcttcc gggcaattgg tcaagccttc caggtgttca
     5941 gttatctgtg acagatctgg caccacgatg gcgtggcgtg acgggccata cagcgctgag
     6001 aagtacggcg catcttccag gctaacgtcg tcataaattt ctgacagcag cacaccacca
     6061 aaacgctccg ccagcgcgtt cagacgctga tcttcagagc cgccaggctg gcttaaacgt
     6121 tcgatctctt catcgacggc gtttttgcgc gcgcccactt catcgcgttc aacaatcgcc
     6181 tctcgctcac gctccagcaa ctgttgcaga tattcggtga cgtcctggct ggaggtaaac
     6241 tcttcgccgc actgttcgct caactggttg agactgtttt gcgctgccag ccaaaccggc
     6301 gcacgctgca tcaaactctg aatgcgagac tgcagctgtt cctgttcctg gcgcagtgcc
     6361 atgcgctctt cacgggcgtt agacacgcta tcggaaagag aggcaatgcg cgcttccagc
     6421 tcctgatgca gagcttccag ctcgtcaatg tcaaaattct tgccctgacg tttgcagaaa
     6481 tctgccagca gacgctcagc ttcttgctgt tcgcgcagac gctgttccag ttcgcttaag
     6541 cgcatccgca acggctgaac ctgctctgcc aggtgacgct gatcgacccc ttcgcgcaat
     6601 agttcgcgag cgacatccca cgcctcgtta cgcgccagcg ggccgttgat tgccaccacc
     6661 agctgataag cctgctcaaa ctggctgtgc gcggtttgcg ccatgctcat tttctgctca
     6721 agagagagca ttttttcagt cgcttccagc tctttcgcct ggaaggtttc cagccattcg
     6781 gcggcgctgt cggcggttaa gtccggcaga tggcacagtt ctttggcgcg attaagcgca
     6841 gcaatcgcct ggttatactg gatcgcgcgc gtttgctgga cgtccagcgc ctgctggtag
     6901 ttagcaagct ggcttttcag ctcgtccact tccagttcgg cagcttccgc gcgagcctcg
     6961 ttctcttcct ggcgttcgat ggcttctgcc accacttcgt tttgctcttc cagacggatc
     7021 tgcagctcat cgagatccgc ttcgtagcgt tcgatcttct cctgctgacg cagtgcggtt
     7081 tgcaccaggt tcaggtgatc gctggccgcc tgataatccg cttccagatc gccttcggca
     7141 ccgttgtgct ctgccagctc acgcgccata tcgacgtgtt tgtactgctc agccgccagt
     7201 tgctgacgcg aagtatgtaa ctcgcgacga aactccaggg ctttgtcgag atggacacgg
     7261 cgctcgttgg cgtgacgcat gtagtccgcc gccacgtagt tggtagcttc gctgatcaga
     7321 tgcttaaaca ggtcacggtc cgactgggtg acacgaatcg cttccagcgt catgcggttt
     7381 tcacgcagcg ccgcttccat gtcctggaac gctttacgca cgccgctgtt ttccggcaac
     7441 aggtagtcgc gcagagaacg ggtaatggca ctggagatcc cgccatacag cgaagcttcg
     7501 atcagacgat agaatttgct acggtcagat gcggagcgca gacgacgcgc gatgatgccc
     7561 agatcgaaca tcagcgagtg gtaatcggta atggagttga actgtttaaa ctgcacgcct
     7621 tccatcgcct cgagcttgtc tttcagctcg ttaagcggca gcacgcgcgc ctggcgttcg
     7681 ttcagggttt cggtcaccag ctgtgtcggc tgcaccgaca tcggcagtcc ctgaatggca
     7741 aatggtttga tatccacttt acgatcgcgt ccggcaacct gttgcagacg cacaccgacc
     7801 accacgcgct ggtggcgcga gttaatggtg tcgagcatcg aataacagac acccgctttc
     7861 agcttaccgt gcagaccttt atcgcgcgaa ccgctggtgg ccccggcttc cgtagtgtta
     7921 cggaaatgca gcagggtcag gtcggggatc agcgccgtaa cgaacgccgc catggtggtg
     7981 gatttacccg ccccgttacc gccagaaagc gtcgtgacca gctcgtcaag gtcaaaagtt
     8041 cgggcaaaaa agccgttcca gttaatcagc gtcagtgagc gaaatttacc gcgttcaatc
     8101 attattcttc ctctccgcta tctggctgat tttcttcggt ttcatcgttg agttgcagat
     8161 gattttcaat cggcattgct tcgccatcac gaatcaggcg acgctgtgct tcacggggat
     8221 cgtcgccagc acgcacatcg gctccgaagc ggaacaccga ttcggtaatg cgaaacttgc
     8281 tgctgtcgtg gcccataaac cacaccatgc ctaaacgacg cagacggttg agcgaagaac
     8341 gtactttctc ctgcaacttc tgacggtcaa cgtctgaacc ggttgaacgg ttgttcacca
     8401 gtttcagcag ttttgcttca tcggccaggg tgagcagttc gtcgtacagt tcctgctggg
     8461 tgaaaatccc ctcattcgcc agccgttccg ggctgagata gagataacag aggattttcc
     8521 cgaccatcat atccagttcc gacaagacgg aacgagggat cagcgtggtg gaacgtgggc
     8581 gtaaatagaa gaacccttct ggtgcgcgaa taagctcaac gttataacgc gcgtaaaact
     8641 cttccagata ttcctgaaaa tccatcagga atgcatgatt atccagttcg tcgaggccaa
     8701 tatggcggcc tgaacgtaag gcgctgtcca gcgccggaaa taacggattc gccagcgcct
     8761 gcgccagctt aaccggcatc acttgttcaa tatttgtcga tgacatgcgc ctgtaccttg
     8821 gctccgtaat cattaatcgg ctgccatttc gctggcagtc cggtgaaatc tgcttgcgct
     8881 acgccaagac gtaccgcctg atcaataaca atacgcgcaa cgtcaaagtg acgtgcacgc
     8941 ggatactgtg acagatattc gcgtaccacc agaccaagat ccagcggcac ttgtctggtt
     9001 ttgtacacgg caagttgttc ttcgatgatc gccgccagct gttcgcggat ctcgttaaac
     9061 tcttcgtatt ccagatcctc aggaagttcc ccagtcactt cttcatcgcg cagtgccatc
     9121 tcttcgtcac gcatatccag cagacgatcg gcattggcat aagttagcgc ccacggctca
     9181 tcaaaatagg tttgtaccga ctgacgtaac cgctgagcaa agacgcggtt tttatccata
     9241 tcgatcgcgg tacgaataaa tttgtgtacg tggcggtcgt agccaatcca caagtcgatg
     9301 gattgctggc cccaactgat aatacgatcg agtttgctct gcagatcgaa caccagacga
     9361 tcgacgaaat gcagatcgtc atgggtcatc gtcgcatcct gaatgcgcaa cagattagcc
     9421 tgcaatttgt cgcctgccgc ttccagcgta tcctgcaatt cacgcagcgt cctggaagtt
     9481 tccgaaagca acaattcaca gctggaaatc gccgcccgcc agtctttgtt cagcaactgg
     9541 gcgatatcgt ccttcacctg ctgctgctgt tcgtccatca gacgttgcgt caggtcgata
     9601 ctgtcgaaaa tttctgctac cgaatatttc agtggcgcat agacattacg gtgccagtga
     9661 aattcatcac cgccctcttc ggcggcatct gctgcgcgtt tgagctcacc cgccacaatc
     9721 gacaactgca tagaaagacg cagcgtagaa aactcgcgct gacggatgta gtagtcagta
     9781 atgccgatgc cgagcggcgt cagacggtaa attgcgttcc cttccgcctg ctcgctggta
     9841 aagcggttca gcagacgttg acgcaccatg tcgttgatcg cgttattggc gcgcacgccg
     9901 atggtttcgc tggtttgctc aaacgcatca ctcacatggc ggaatgcatc caccagctcg
     9961 ccttcactca tctcaccatc cagacgctcg ccgttcagcg tggcaaccgc cagcagaaaa
    10021 gagagtcggt ctaccggcag cgagatggag aagtcatttt ttctggccca ggcaaccagt
    10081 tcggggactg tctgggaaaa ttcactcata ctttatcctt gctctgcggt ttgcgcgcgg
    10141 tgacatgaat ataacgcccc agggtaatat acggttcctg acggcaataa cgcgtttcta
    10201 attcaagtaa tgcttcatag cagtcgcgct gctggtgctt ctcgcgcaga taatcatgaa
    10261 acacgcgaac gcctgtctta cccataattt gccaaccagc ttcttccagc cacagataaa
    10321 cctgcgccgg gtcgcgtgga taatctggcg aaagcgtccg ttttttcttt ttcggcattc
    10381 ccgcctgcac gtaatcaaaa ttcccggcga ccatgttatg catcaacaaa ccatgcgcat
    10441 tgtagaacat taacgacaac acgccgcctg gacgcaacac tgaccacagg gtctgcaata
    10501 cactgcgggg atcggccacc cactcgagca ccgcatggaa caatatcaga tcaacgggcg
    10561 tttccaaatg cgaagcaaca tcctgagcgg cgcaatgtat aaattgcatg ttgtcgctca
    10621 caccttttgc ttctgccgcc tgttttgcgc ggtcgatcat ctgcgcagaa agatcgcata
    10681 aaatgacctg atgcccacgc tcggccattt tgattgcggt ctgcccttct ccaccgccag
    10741 catccagcac acgcagtttt tgcgggccca tttccgccag cacgcgatcg agatcctgcc
    10801 acagaatagc ctgtcgaagc tgccctttgg tggtgccgta aatgttacgg gaaaactttt
    10861 ccgcaatatc atcaaaattg cgatcctgca ttgctttctc caccggttgg ctaagacgtt
    10921 ttctgataaa gacgctattt tgtcacagcc acggcgagaa tgaacccgtc tggtgtaata
    10981 tcgctggcga tcccttgcta tatggttaaa aaaggaacca gaaaggatgc tttttacact
    11041 gaaaaaagtg attggcaata tgttgctgcc acttccgctg atgttgttaa ttattggcgc
    11101 tggcctggcg ctgctgtggt ttagccgttt tcaaaaaacc gggaagattt ttattagcat
    11161 tgggtggctg gcactcttgt tattgagttt gcaaccggta gccgaccgcc tgctgcgtcc
    11221 catcgaaagc acctatccga catggaataa ttcgcaaaaa gtggactata tcgtggtgct
    11281 cggagatggt tatacctgga acccgcagtg ggcaccgagc tctaatttaa tcaataacag
    11341 tctgccacgc ctgaatgaag gtattcgctt atggcgcgaa aatccgggat caaaactgat
    11401 cttcacggga ggcgtagcaa aaaccaatac ggtgagtaca gcggaagtag gtgccagagt
    11461 tgcgcaatcg ctgggcgtgc cgcgcgagca aattatcacc ctggatttgc caaaagatac
    11521 cgaagaagaa gctgcggcag tgaaacaggc gattggtgac gcccctttcc tgctggtgac
    11581 ctccgcatca catctgccgc gggcgatgat cttttttcag caggaagggt taaatccgct
    11641 accagccccg gcgaatcagt tggccatcga ctcgccgctt aacccgtggg aacgggcaat
    11701 cccttctccg gtctggctga tgcatagcga tcgtgttggc tatgaaacgc ttggccgcat
    11761 ctggcagtgg ctgaaaggtc cgtcaggcga gccacggcaa gagtgatttt gatgccagat
    11821 cgaagttgcg ccgactaaat cgtccggtat taaccagttg cgcaacttca tcccataaca
    11881 catacaacca acgccgccag atgaacgact ccgccactgg cgcgcgttgc aggtaactcc
    11941 agagcaaatc ttccgccaga gaattatcca ttagtcggaa cagttcgtat tctcgcggtg
    12001 cccaaagcat tagtcccggc ccgaccatcg ccagtaactg atcgctgcgc gaatctttca
    12061 gcatgctgcg taaacagaag ttaccgtgaa tcaacacaca attgtcgtta aagccttcaa
    12121 ataatgccgg gagacattcg cgagtgcgaa acaggatccg cttatcctgc atcgtcagac
    12181 cggtgttatt gaactgattg agcgtggtcc atagcacttc aacatgttgc aggtaccatg
    12241 agggccagaa attttcctga gtattgtcga ccgcgccgac gcaaccgcga ctgtcctgac
    12301 ggtgccaggc cagtaaggct tcaacgattt ggtctttgag ctgttcccag cgttctggtg
    12361 ttcgggctgg tgcctccacc gaaacaccac gcatccgctc cagtagcagg acatcagggc
    12421 cggggtgttc ttcatgcgtc atcacaccat aaatagtcgg catacggaca gtcccacttc
    12481 ttgccagcat ggtggttttc catgccagtt gtcgggcttt tccgggcgta ctaaagcttc
    12541 ttgccattaa cggcattggg tttccctggc tgtcatacaa agcccacaac gccgtatccg
    12601 ctttttcatt gacgcactca atacgactga gtttttcgcc cagtaaatgg cttaattcgg
    12661 cacgcagctg ttccattcag attaccctca taaaagctac tgctttcata atgagcgcca
    12721 atataaaaga tgtcacctga atgagatcaa ttgttgaaaa gaaaaattga ggaaaatcgt
    12781 aacggtacga cactcctccc aaaattggct gaagtgtcgt gaagtgaaat tagcgcattt
    12841 cagcgcgaac gcgctcaaga tcttcagggg tatccacacc tgtgccagga acttcctgag
    12901 caacagcaac atggattttt tcgccgtacc acagaacacg aagctgctct aacatttcga
    12961 tgtgttctaa cggacttgcc tgccagttga cgtaacgacg gataaagcct gcacggtagc
    13021 cataaatacc aagatgacgc aggaagttat cgccaacggt ttcaaggcct tctgcaaaac
    13081 gatcacgatc ccaaggaatg gtggcgcgtg agaagtacag tgcataccct tcagcgtcga
    13141 gaaccacttt caccgcattc gggttaaacg cttcttccgc attgtggatt ggcaccgcca
    13201 gagtcgccat acccacctga cgctgagcga ggttatcagc aacctgacga atgattgtcg
    13261 cagggatcat cggttcatca ccctgcacat taacgatcac cgtgtcgtcg ctgaatgcgc
    13321 atttttcgac aacttccgcc agacgttctg ttcctgactg atgatcggcg cgcgtcatac
    13381 atacttcacc gccagcggct tcaacggcgc gggcaacatc ctcatgatcg gttgccacga
    13441 tgatgcgctc ggcacctgat tcacgcgcgc gttcaagaac atgaacaatc atgggtttgc
    13501 cgttaatatc aaccaatggt ttaccgggca gacgcgtgga cgcgtagcgc gcgggaataa
    13561 tgaccacaaa actcatgatt tactctcatc agcagtcagc acgcgggctt ccgtttccag
    13621 caacactgga atgccatcgc ggagcgggaa agccaggtta tccagtttgc aaataagctc
    13681 ttgtttttcc tggttatacc aaagttttcc gttgcaaacc gggcaggcaa tgatttcaag
    13741 caaacgatga tccatagttc ctccagatgg atcgggttat gaatgcataa atcttatcat
    13801 aatcatttat gcgacggggt ctatttccca gccagtgcgg caatcagtaa agagaccttg
    13861 cgggcagcgt cctaatgtca cccgcgttgc ctgctgccag ctcgcgaaat cagtaatcgc
    13921 ctgacgtaac cctttttgca gcattgtcgt tggtttaatg ccttcctgta accacagaga
    13981 gataacttca aggatgcctg tctggcgatg cattttggca tccattcgcc caactaattg
    14041 cccacgatgt aataatggca gaacaaaata gccatactgg cgtttcggcg ctggggtata
    14101 gcactccagc cggtagctaa aatcaaaaag ctgctctgcg cgtttgcgat cccagacaac
    14161 aggatcaaaa ggcgaaagta ccgcgctgtg cgttgcagtg agctttccgg ccagcgcacg
    14221 ctcgagtagc ggcagcaaat cagcatgcag ccaaagattg cccaattttt caacatgcac
    14281 agcaatgatt tgctgctgtt cagccctcgc ttcgcgccac gccgccagtg ccggacgttt
    14341 cagccgatag taatctgcca gccactgttc gcggaatatt cccaggctac gcgcactgtt
    14401 atccagcatg atgatttctg cttctgtttg cgaaacgaga tcgcgctcat catcccagtc
    14461 aggcatgaca cggtgggtta aatcataaac gcgctggaag ttgcgccgtt caatcaccat
    14521 cacctttccg gcagtaaata aaccttccag atgccgttta tgcggcttcc attcccacca
    14581 gccgcttgca cctttacgag gatgctcaaa atcggctgaa cgtaccggcc ccttatcatg
    14641 aatatgctga attaactgtg caatttccgc ctcatgttcc tgcatccagg cgtctttgta
    14701 tttccagccc attttttcag gtgccagcat gcggtggcga ataagacgaa agtcgctacg
    14761 cggcataaag caggcttcat gcgcccagta ttccattaat tcgccacgcg ccagagactc
    14821 atccagccac tgggcaggat aatttcccag acgactgaaa agcaccagat atggactacg
    14881 ggcaacaata ttgatggtat cgatttgcag caaggacatg cgggagatcg ttgccggaat
    14941 atcctccaac gacgcttgac ggcggggttt gtttaacagg ccttgtgcgg caaggtgaag
    15001 attacgcgca tcagcaaggg agaggtgcgg cagcgacatt catgactcca tcaatcgaac
    15061 gctgccgcgg cgtaactagt tgccagaagc cagcgaggtt agttgcgtaa gcagtttcgc
    15121 tggttcatca cctgaaagct gtgcgtctac aggcaaatac caccaatttt cttctgcaaa
    15181 ggcccggcat ttcaccgcat ctttttcagt cattaccagc gtttgcccgg cgcttaccaa
    15241 cgcactgaca tccgcatggt tcaaagactg atgatcggcc agcggtacac atttttccgg
    15301 ttgtacgcca cacatcttca gcgtggcaaa aaagcgcggc ggatgcccaa tccccgccat
    15361 cgccactaca tgttcaagct gagcaacgtc acaacgcgta ccggtacgta aattcaccgc
    15421 ctgacccggc agcagatgca tggggatttc accgctgcga gggacaccgc cgttgacgat
    15481 taccgcatca accgacttta agcgccccgc tcgctcacgc attggccccg ccggcaacca
    15541 ccagccattg ccaaagcgac gcacaccatc aataacgaca atttccacat cacgcgccag
    15601 acggtaatgc tgtaaaccgt cgtcggttac gatgatctgc acatcagggt gttgcgccag
    15661 aatggctttt accgcatcag aacgaacggg agaaaccgca acaggcgcat cagtgcgttg
    15721 ataaatcaac acaggttcat cacccgcctg tgctgttgtg gtatctgccg acaataacag
    15781 cggataagat tcagccttac caccatatcc ccgcgatacg acccccacgc gaataccgcg
    15841 ctgttgcaac tgttccacca gccagacaac gaccggggtt tttccgttgc cgcctgcggt
    15901 gagattacca accacgacaa ccggtacggg ggcacgccag gcgcgcttca gttttagttt
    15961 atagcaaaga cggatcgcgc cacttaccag gccatacaac caggagagtg gcagcaatag
    16021 ccgccacaaa ggggattcac cagaccagat tttttcgatc attggccaaa ctgcattttg
    16081 tgaagttgcg cgtaaacgcc gcggtgctca agcaaatcgt tatgcgtacc gcgttccaca
    16141 atgacaccat cctcgacgac cacgatttcg tctgcctttt caatggtaga caagcggtgg
    16201 gcaatcacca gagaggtacg gtttttctgc aactcatcca gtgccgcctg aatcgcacgt
    16261 tcggattcgg tatccagagc cgaggtagct tcatccagaa tcagaatcgg gctatcacgc
    16321 agcaaggctc gagcgatagc aatacgctga cgctgaccgc cagagagcag cacgccgttt
    16381 tcaccaatca ctgtatcgag accgttatcc atcttattga tgaagtccat ggcgtaggcc
    16441 atacgcgccg cttcttcaat ttgctcacgg ctgtactgtt cagtccgtgc gtaagcaatg
    16501 ttgttagcaa ccgtatcgtt aaacagatgg acattctgcg acaccagagc aacctggtta
    16561 cgtaacgacg ccagggtata ctcgcgcaga tcgtgaccat ccatcaggat ttcgccttca
    16621 tcaatatcgt aaaaacgagt gatcaggctg gcgatggttg atttacctga accagagcgt
    16681 ccaaccagag caaccgtctt ccctgccgga attttcaggt tgatgttacg caatgcaggt
    16741 acgtcacgtc ccggataagt aaaggtgaca ttgcggaatt ccacgtcgcc agtcgcacgc
    16801 tcgatcacgc gcttaccttc gtctttctcc tgctcactgt ccagaatggt aaacagcgtc
    16861 tgacaagccg ccataccgcg ctggaactgg gcgttaacgt tggtcagtga tttcagcgga
    16921 cgcatcagtg caatcattga agagaaaaca acggtaatcg taccggcagt caggctatcc
    16981 atgacacttg ggaagctcgc cgcatacaga acaaacgcca gcgccaaaga ggcgatcagc
    17041 tgaatgatcg gatcagagat ggaagaggct gaaaccattt tcatcccctg aagacgcatt
    17101 cggttgctga ctttatcaaa gcgtttcgtt tccacttcct gaccaccgaa aatcaatact
    17161 tctttgtggc ccttcagcat ttgttcggcg ctggtggtca cctgccccat ggtgttctgc
    17221 atgtttttac tgatgttgcg aaaacgcttc gatactacgc gaatcgcaat cgaaacaatc
    17281 ggtgccagca caatcaaaat gatcgacagt tgccaactgt aatagaacat catgatgaac
    17341 aggccgatga ttgacgcacc ttcacgcaca accgttatca gtgcgccgga agaagaagaa
    17401 gcaacctgtt cggaatcgta ggtgatacgt gacaacagcg tccccgttga ctgtttgtca
    17461 aaaaatgaaa ccggcattcc catcatgtga ccaaacaggc ggcgacgcat ggtcattacc
    17521 acctttcctg atacccagga gatacagtag ctggagacat agctggtgat accacgtaaa
    17581 atcatcagcc cgatcaccac cagcggcatc cacaccagca cggagcgatc tgttttacca
    17641 aagccatcat caagaagtgg cttaaggagc gataacatga aggtatcgct ggctgcgttg
    17701 aggattaacg ctacgcccgc cacgatcaga cccgctttaa aaggcgcaat ggttggccac
    17761 agtcggcgga acgtctgcca cgtagagaga tctttgtcgt tatgcattca aaaaaccagc
    17821 atttgttgaa atagccgcat attctacccg ttatccactg gcacgccaaa ccactgatga
    17881 taccaacgag gtaaaatttg ctctctgagg ctgctaatcc gccagccttg cgctgaaaaa
    17941 ttgaccgtca cttgaccctg atgtggagta tcaagccatt gatatccttg ctgttgatag
    18001 cgatgcttaa ctttattaga gggcaatcgc catgcgttat agcgcgatgc cgatgcgagt
    18061 gccacttttc cattcactcg ctgaattaat ggcaacgatg atgaggtatt actgccatgg
    18121 tgaggtacct gaagcaatgt tgtctgcact tgctgccagt aacggcttag catcttttgt
    18181 tcagcgggga cttcaatatc accggttaga agaatgctat tcgtcccgtc atcaacctta
    18241 accacacagg aatggttatt tcctttatcg ttgctagctt gtaaaggcca gtgcacgctg
    18301 aaacgcaatc cttgccattg ccacgcttcg ccacgcacac agggctgatg atgttcccag
    18361 tttaacggac ttctgatcca taacatcggc catgtgtgca atattgagtc cagccctccc
    18421 cggtgatcca gatgttcatg actcagaata acgccttccg gttcaagatt atgccagtgg
    18481 agccagggga taatcagttg ttgcccacta tccccttcgg gccaggccag tcctgtgtca
    18541 tagagaatcg ctttgccgtt tctggctatc accattgcca gcccttgccc gacatcaagc
    18601 atgtacacct gccactcgtc aggtcgaggt ttttgccaca gcggccagca catcagcaag
    18661 cctacagcca cacacattgc tggcaacgtt cgccaggcgt ttaatcgcca taccaccagt
    18721 aagaaccatg gggaaaatga tagccattgc caacgttcag caatgttgat ccacccttct
    18781 ggcaaactct ttaaccccca gaaaagtaaa gccaaagacc ggtcggcaag aaaccataac
    18841 ccttgctcca ggattaacgg cccgcttaaa tgcacaacca tcgcggcgag gatcaacgga
    18901 accgtgataa atgtcaccaa gggaattgct aatagatttg caataaacga ggtcagacta
    18961 atgccatgaa atatgacgat ttgcacaggc ataagcagga gtgtgattcc cagttgcaaa
    19021 tgaatgaggg aaacaactgc acgcaatacc ggcggcagtt gccactcagg acagggaaac
    19081 cactggtacc aaaaaatcag tgccgcgacc gcagcggcag agagccataa actttgcgag
    19141 agaatggcaa caggatccat cagcaaaatt gccgccagac aacatatcca tacatcccag
    19201 ccactccact gtcgcccact taacttaagc attccccacg tagcaagcgc caccacggta
    19261 cgcaatgcag gaggttgcat tcccgtcaac caggcataaa aagcagcaca gcagattcca
    19321 ccaattaatg gcatttgcca gtggatccag cgcccaggca gataaacttg cccaccgcga
    19381 atgagtccgg cagccagcaa cgccgcaaaa gcgatgtgca atcccgatat cgccattaaa
    19441 tgcgccgttc cagtatcacg catgatattt ttgatttctt tggggacgga taaccgttcc
    19501 cccataccta aaccaagaat aaccgcattc cacatatagg gttgcagcgt tgtttgtagt
    19561 gacgccagat actgtgcacg caggctacaa ttcggttcaa tgacacttgc ctgcagaaag
    19621 cggccggtga gcggctgatg ctgggcaatg gcataacgct ggctatcaaa gccgccatca
    19681 ttaagttggc catgaactgc acgaactttg agtttcattg accattgttg tccagcacaa
    19741 accgcttgcg gaagatattc gccatacagc acaaggcctg gcgcagggaa tatacgtcga
    19801 ccttgtagat gagtaatttg accgtaatga gtggtcatgc cgtcagttgc agtgatctca
    19861 acaattgcat cctgcgtcgc gccagttaag gtttctcctg cccacaaaat ttgctttgct
    19921 gataatatgc cccaaacgaa aaacagcaac gtcagcgcga tataacggac ggttttaaca
    19981 gggataaatg ccagtacgca ggcgaagaga gtcagaaacg caagggttaa tgtcccaggc
    20041 aattggggca aaatcagcaa cggaaaaatt ccgcaaatta tgcatacgcc gactgtcgtt
    20101 attttcatgc acccctccct gtttatggaa agtgtgcaac tttgttgagc ggttgtctgg
    20161 tgggaatttt gcgttccccg aggcacattc agaagaattt aacgaaaggg tgaaaactgc
    20221 tcaaaactct gcgaggcagc ttccagttcc tgattagaga aatgaaaaaa gcacccgaca
    20281 ggtgcttttc tcgcgttcaa gtttgagtaa aaacttaacc gtaaatattg gcgcgatcgc
    20341 gcagttcttt accaggttta aagtgaggaa cgtattttcc ttccagttct actttatcgc
    20401 cagtcttcgg attacgtccg gtacgtggtg cgcggtagtg caaagagaaa ctgccgaaac
    20461 cgcggatttc aatacgctcg ccctgcgcaa gagtcgaggc catatgctcc agcatctctt
    20521 ttactgcatc ttcaaccgtc ttggcgggaa tgtgcgattg ctgggtggca agtctttcta
    20581 tcaattctga cttggtcatg attcctccgg ttccttaaag gcaaattaag cggctgctgt
    20641 tgctgcattg attaagggcg gccgtagccg cccttagtgc ttgattacag gacgaaacct
    20701 gcaatctgtc aagtaaactc aacaaacttc ggaataaaaa tcccgaagag tcagagaatt
    20761 actcgccttt agctgctttg aaagcttcag ccattgcgtt gttggagaag tttgcatctt
    20821 cctgtttgtt aacagttgcg attgcatctt tctcgtcagc ttcgtctttc gcacgaacag
    20881 acaggctgat tgcgcggttt ttacgatcaa cgccggtgaa tttagcttca acttcgtcgc
    20941 caacgctcag aaccagggta gcgtcttcaa cgcggtcacg ggatgcttca gaagcacgca
    21001 ggtaaccttc aacgccgtca gccagttcta cggttgcgcc tttagcgtca actgcagtta
    21061 ctttaccggt tacgatagcg cctttcttgt tcagagcaac ccagttgttg aacggatctt
    21121 ctgcgagctg tttaacgccc agggagatac gttcacgttc tgcgtcaacc tgcagaacaa
    21181 ctgcagcgat ttcgtcgcct tttttgtatt cacgaactgc ttcttcgcct gcaacgttcc
    21241 aggagatgtc agacaggtga accaggccgt cgatgccgcc gtccaggccg atgaagatac
    21301 cgaagtcagt gatagacttg attttacctt caacacggtc gcccttgttg tgggtttccg
    21361 cgaactgctg ccacgggtta gctttgcact gtttcagacc cagggagata cgacgacgtt
    21421 cttcgtcgat atccagaacc ataacttcca ctacatcgcc aacgttaaca actttggacg
    21481 ggtggatgtt tttgttggtc caatccattt cggaaacgtg taccaggcct tcaacgcctt
    21541 cttcgatttc aacgaagcag ccgtagtcgg tcaggttggt cacgcgacca gtcagtttgg
    21601 taccttccgg ataacgttta gcgatagcta cccacggatc ttcgcccaac tgtttcaggc
    21661 ccagggatac acgggtacgt tcgcggtcga acttcagcac tttaacagtg atttcgtcgc
    21721 ccacgttgac gatttcgctc ggatgcttaa cgcgtttcca agccatgtca gtgatgtgca
    21781 gcaggccgtc aacgccgccc agatcaacga atgcaccgta gtcagtgagg ttcttaacga
    21841 tacctttaac ttccatgcct tcctgcaggt tttccagcag ctgatcgcgc tctgcgctgt
    21901 tttcggattc gataacggca cgacgagaaa caacaacgtt gttgcgcttc tgatccagct
    21961 tgattacttt aaattcaagc tctttgcctt ccaggtgcag agtgtcacgc accggacgaa
    22021 cgtctaccag agaacctggc aggaacgcac gaataccgtt cagctcaaca gtgaagccgc
    22081 ccttaacttt gccgttgata acaccggtaa cagtttcagc atcttcgtaa gctttttcca
    22141 gcgtgatcca ggcttcgtga cgtttagctt tctcacggga cagcagagtt tcaccgaagc
    22201 cgtcttctac tgcgtccaga gcaacgtcaa cttcatcacc tacctggatt tccagctcgc
    22261 cctgggcgtt tttgaactgc tcagccggga tggcggactc agatttcaga ccagcgtcaa
    22321 ccagtactac gtctttgtcg atagcaacaa caacgccacg aacgatagaa cccgggcggg
    22381 tttcgatttc ttttaaggac tcttcaaaga gttgagcaaa agattcagtc atgtttaatc
    22441 ttcaggttta tatttaacgt ccacctggct ccatgccgga tggggttgtt taacataccc
    22501 gctgtcattc cattgcaacg ggggtactgc aaattcggtc gcttatgcga gagccaattt
    22561 ctggcgcgcg tattgtagcg ctttttcaat cacttgctca atgcttaagg tggtggaatc
    22621 caacactaaa gcatcggctg ccggaaccag tggcgctacc gctcggttac gatcgcggtc
    22681 gtcgcgttct ttgatctcgg ccaaaaggcg ctcaaagtta acactaaagc ccttctcctg
    22741 caactgtagc atgcggcgat gcgcacgttc ttccgaggag gcgtcaagga aaattttcac
    22801 tggcgcatca gggaatacca ccgttcccat gtcgcggcca tcggcaatca ggccgggtaa
    22861 ttcgcgaaac gcgcgttggc gacgcaataa tgcttcacga acgcgtggga atgccgcgac
    22921 ttgtgaagct gcattcgcca cttcctgagt acgaatttcg ccgctgacat cttccccttc
    22981 gaggatcact tccagattgc cattggttga cacaaaacgt acatccagat gggatgccag
    23041 cggtaccagc gcatcttctg acgcaacatc aacatggtga tgtaatgccg ccaatgccag
    23101 tacgcgataa attgcacccg aatccagcag atgccattgc aatgcttccg ccatagcctt
    23161 acacaaggtg cctttccctg caccgcttgg gccatcaatg gtaataaccg gggcaattgc
    23221 cgtcatcttt atctccttaa aacaggcgta ccgttaacat aaccgcgcgc attatacgcg
    23281 ccgacgccac aaatcgttaa cgttgtgtgc aaaaaccagt tttgtctgta ttcagatggg
    23341 gatttattgc gcaattatag cgggctggca gaagaccagc ccgaaagatg acaatgattt
    23401 acttaccaga ggcgatacga tcacggatgt gttgcgcacg ctctgttgat ggtgggtgag
    23461 agtcaaacat ggactgggtg cgaccaccat ccagactagc cagtttttca aagctgccaa
    23521 ccagcccctg ggtgctgatg ccacgtttct tcaacagatc aaaggagaaa tcatctgctt
    23581 cggactcttt atcacgggag tatttagcat taatagcgcc ttctgcgata tcacccaatt
    23641 gtgagcggga aagctgggaa gccacaccgc tggtagctga aatggcatcg cgtgcggcaa
    23701 cgatcgcata agaagctttc atttcagcca gcgagtgacc caacgcgacg tggcccagtt
    23761 catggcccag aacaccttca atttcgttgt cattcatcat gtccatcagg ccactgtaga
    23821 cacggacaca gccgttcgcc atcgcccatg cgttgacgtc gctggtcata taaaccttat
    23881 agttgaccgg cgtgccatta atattgttac ccaatgcttt ggcgattttt gccagacgtt
    23941 taccgtactt gctggattta cttgcgactt gattgccgct gtccatttcg gcacagccct
    24001 gattggcaat cgcttttgca tcggcatcgc ttaatgttgc tgctttatag gcatttaaac
    24061 cggagctgat agccatattg gtatcaatac cgtgggtatt ttgacaccct gtcagtaatg
    24121 ctgcagaggt cgcaatcgcc agcagtaatt tagtattctt catgtgaatc atccgttgat
    24181 taatcatata taaatattat gtatcaacaa cttacattga caagcgacgc taccattaat
    24241 caaaatgact cacaaggtcc gaaaaaaaac gccgacaatg tgccgacgtt ttgataaaga
    24301 aagatttggc tatttattgc ccgttgttca ttcaggctgc ctggctaatc cgcgccaact
    24361 gctcgaaata atccggaaat gttttggccg tacatttggg atcaagaatc gtcactggtg
    24421 tatctgacaa cgccaccagc gagaaacaca tcgccatccg gtgatcattg tacgtcgcga
    24481 tctcggcaaa gttcagtttt tccggtggag tgatacgaat gtaatcgtgc ccctcttcta
    24541 cttccgcacc gactttacgc agttctgttg ccatcgcaaa caggcgatcg gtttctttaa
    24601 cacgccagtt atagatattg cgcagcgtgg tggtgccttt tgcaaataac gccgccgtgg
    24661 caatggtcat cgccgcatcg ggaatatggt tcatatccat atcaatagcg ttcagttcac
    24721 cacgcgtgca ggaaatataa tcatcgcccc agcaaatggt cgcgcccatt ttttccagca
    24781 catcagcaaa gcgaatatca ccctgcatac tgttacgtcc aataccggtc acttttacag
    24841 tgccgccttt gattgctgct gctgccagaa agtaagaagc cgaagatgca tcgccttcga
    24901 ccaaataagt acccggagac tgataagact gcccgccttt tacgacaaat tgttgatagt
    24961 gctgattttc aatttcaaca ccaaacgtct tcatcagatt gagtgtgatg tcgatataag
    25021 gtttagaaac cagatcgcct ttaatacgaa tcaccgtatc ttccggcgca agaggcgcag
    25081 tcattaacag tgcggtgagg aattggctgg aaacggagcc atcaacgtca acgttgccgc
    25141 cagtaaagcc gccctgtaaa cgcaacggcg gataattttc ttgttccagg taagtgatct
    25201 tcgccccgcc caggcgcagc gcatccacca gatgaccaat cgggcgttct ttcatacgcg
    25261 gctcaccggt cagcacaata tcattgctac ccagacaaag agctgccgcc agcggacgca
    25321 ttgccgttcc ggcgttaccg aggaacaact ccagggcacc ttctgcgtgt aatggaccgc
    25381 cgttaccgat aatttcgcaa cgcgtacgat cggctgaaag cgtatagctt acccctaacg
    25441 ctgttaatgc attcagcata tggcgcacgt catcgctatc cagcagattg gttaatactg
    25501 ttttgccgtg tgctaatgcc gccagcaata aagcgcggtt agaaacgctc ttggaaccgg
    25561 gcagattaat agtgccatcg acacgagcga tgggttgtaa cgtcagggat tccatgaact
    25621 caactctcta caacagaaat aaaaacccca caggctggct gtggggatta agcaaaattt
    25681 cggcattaac cgtgacggcg ttcgaactca accatgaagt ctgtcagcgc tttaacgcct
    25741 tccagcggca tggcgttata aatagaagcg cgcattccgc cgaccacacg gtgacctttc
    25801 agtgcatgaa ggccagcagc aaaagactct tcaaggaaca atttgtcaag cgcactgtcc
    25861 gccaactgga acggcacgtt catccgcgaa cggttagctt tcgccacgtc attgcggtag
    25921 aaatcgctgt tatcaatcac cccatatagc agttctgctt tttgctgatt gattttatcc
    25981 atttcagcta caccgccgtt cgctttcagc catttaaaga ccagaccaga tagataccag
    26041 gcaaatgtcg gcggcgtgtt aaacatggag ccgttatcgt tgaggatgga ataatccaga
    26101 atcgacggac acgcgatatt cgctttgccc agcaaatctt cacgaacgat gacgattgtc
    26161 aggccagccg ggccgatatt tttctgcgcg ccagcgtaaa tcacgccata acggctgacg
    26221 tcaatcggac gggaaagaat ggttgaagag aagtcggcgg cgaccaccac atctttgccg
    26281 aagtctggcg tttcgtcgat ggcgataccg tcgatggttt cattcgggca gtaatgcata
    26341 taagcagcat tatcagagag ttgccattca cgcattggct taaccgcgcg cagaccatca
    26401 acagtcactt tggcgtcaaa gacattaggc gtgcagtatt ttttcgcttc tttaatggca
    26461 cttgccgccc agtaaccggc atcaacataa tctgcggtgg ttttatcacc gagaatattc
    26521 agcggtaccg cagcaaactg accgcgacca ccgccatggc agaataatac cttgtagttg
    26581 gaggggacat taagaagatc gcgaaaatcc ttctcggctt cctctgcaac ctgaatgaac
    26641 tctttgccac ggtgactcac ttccatcacc gacgtaccaa gaccgttcca gtcgcgcagt
    26701 tcctgttgag cctgtttaag cacctctgcc ggtagcattg ccggaccaga actaaaattg
    26761 aagatttgag ccatttcccc tcaccacgtt gcgttgctat taacccgctg tcaatcgatt
    26821 gcgaccacag gtaatacatt attcctgtgg ctatcggttt tatcattcag tgacacacat
    26881 cgcaatgagt aaaaccgttg agggcaaaaa tgtggcttgc atcacacaaa agaatctctc
    26941 ggattattag caaaaagccg atgtttttac tgtcaggtca cccgttttgc ccgacttgcc
    27001 ttcgtccatt ctggatttgc acagcgcgga cataattgtt tttcccccgc tttcatatga
    27061 atgatttcac tacaacgaat acaggcatag tccgccgact ccggcaccac tttgtaacgc
    27121 tgcgtacaat cactgggtaa aataagtaaa cccggtttca ccttgtcatc ttcaaagaaa
    27181 tagacactga tttgcccgtt ggtttcgaga atcgccagac gtacctgccc cagctgctcc
    27241 acgccacgca atcgtagctc cataaagaac tcaaattccg tcatgttgga gttattgagt
    27301 ttcgaccagg ccagctcgcc atcttcaata ataacaaccg gcttaccttc cagtagatct
    27361 tccagttttt cactgtgcgc catcaaccac attaccaggc ggtataacaa cgccagagtg
    27421 ataaagacga taagtaccgg caccatcggc acatcatcat aaaacgccac atctcccgcc
    27481 gctgaaccca gcgtgagaat gattaacact tcaaacagcg acatctgtcg cacaccgcgt
    27541 cttccggtca ttttgaggaa caaaaaaacc agtacaaagg tataaagact acgtagtgca
    27601 acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca tacggtggag atcgaacgcc
    27661 ttcattttta tgctcttatc gtggtttatc cgctaaaagc atagtcaaca gttcatcggc
    27721 atggcggcgg tgatagcgcc tttgagataa aacccgtatt attgcgcgct ttccgtacga
    27781 ctaaagtgat tttcatgacg caaacattta tccccggcaa agatgccgct ctggaagatt
    27841 ccatcgctcg cttccagcaa aaactttcag acctcggctt tcagattgaa gaggcctcct
    27901 ggctgaatcc tgtgcctaac gtctggtctg tacatattcg cgacaaagag tgcgcactgt
    27961 gttttaccaa cggaaaaggc gcaaccaaaa aagcggcgct ggcttctgca cttggtgaat
    28021 atttcgagcg tctctcaacc aactactttt ttgctgattt ctggctgggc gaaaccatcg
    28081 ccaacggtcc gttcgtgcat tatcccaacg aaaaatggtt cccactgacc gaaaatgacg
    28141 atgtgccaga aggactactc gatgaccgtc tgcgcgcgtt ttatgatccg gagaatgaac
    28201 tgaccggcag catgctgatt gacctacaat ccggtaacga agatcgtggt atttgcggcc
    28261 tgccgtttac gcgtcagtcc gacaatcaga ccgtttatat tccgatgaat atcattggta
    28321 acctgtacgt ctccaacggt atgtccgcag gtaatacccg caacgaagca cgcgttcagg
    28381 gattgtctga agttttcgaa cgctacgtga aaaaccgcat tattgctgaa agcatcagcc
    28441 tgccagagat cccggcagac gtgctggcgc gttacccagc agtagttgaa gccatcgaaa
    28501 cgctggaagc ggagggtttc ccaatcttcg cttatgacgg ttcgcttggc ggccagtatc
    28561 cggtgatttg cgtggtactg ttcaatcctg ctaacggtac ctgctttgcc tctttcggtg
    28621 cgcatcctga ttttggcgta gcactggaac gtaccgtgac cgagctgctg caaggtcgtg
    28681 gcctgaaaga tttggatgtg tttactccgc caaccttcga tgatgaagaa gtcgctgaac
    28741 ataccaacct cgaaacgcac tttatcgatt ccagcggttt aatctcctgg gacctgttca
    28801 agcaggatgc cgattatccg tttgtggact ggaatttctc cggcaccacg gaagaagagt
    28861 ttgctacgct gatggctatc ttcaacaaag aagataaaga agtttatatt gccgattacg
    28921 agcatctggg cgtttatgct tgccgtatta tcgtgcctgg catgtccgat atttatccgg
    28981 ctgaagatct gtggctcgcg aataacagta tgggcagcca tttacgtgaa acgattcttt
    29041 cgctaccagg cagcgagtgg gaaaaagaag attacctgaa cctcatcgag caactggatg
    29101 aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt gggtctggcg accgggtcgg
    29161 ataacggttg gtacaccctg cgtatcggtg aattaaaagc catgctggcg ctggctggtg
    29221 gcgatctgga acaggctctg gtctggaccg aatggacgat ggagtttaac tcatcagtat
    29281 ttagtccgga acgcgccaac tattatcgct gcctgcaaac gttgttatta ctggcacagg
    29341 aagaagatcg ccagccgctg caatatctga atgcgtttgt tcgcatgtac ggcgcagatg
    29401 ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc gttttacggc ctgcaaccgg
    29461 tagatagcga tctgcacgcg tttgctgcac atcagtcgtt gttgaaggcc tacgaaaagc
    29521 tgcagcgcgc caaagcagca ttctgggcaa aataaaatca aatagcctac gcaatgtagg
    29581 cttaatgatt agtctgagtt atattacggg gcgttttttt aatgcc
//
LOCUS       DAZFKM010000056        29136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFKM010000056 DAZFKM010000000
VERSION     DAZFKM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            234..1100
                     /gene="folD"
                     /locus_tag="ACHKAY_002493"
     CDS             234..1100
                     /gene="folD"
                     /locus_tag="ACHKAY_002493"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO5701160.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1102..1314
                     /gene="ybcJ"
                     /locus_tag="ACHKAY_002494"
     CDS             1102..1314
                     /gene="ybcJ"
                     /locus_tag="ACHKAY_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO5701161.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1422..1943
                     /gene="ybcI"
                     /locus_tag="ACHKAY_002495"
     CDS             1422..1943
                     /gene="ybcI"
                     /locus_tag="ACHKAY_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5701162.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1979..3364)
                     /gene="cysS"
                     /locus_tag="ACHKAY_002496"
     CDS             complement(1979..3364)
                     /gene="cysS"
                     /locus_tag="ACHKAY_002496"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO5701163.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3538..4032
                     /gene="ppiB"
                     /locus_tag="ACHKAY_002497"
     CDS             3538..4032
                     /gene="ppiB"
                     /locus_tag="ACHKAY_002497"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO5701164.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4035..4757
                     /gene="lpxH"
                     /locus_tag="ACHKAY_002498"
     CDS             4035..4757
                     /gene="lpxH"
                     /locus_tag="ACHKAY_002498"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO5701165.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4875..5384
                     /gene="purE"
                     /locus_tag="ACHKAY_002499"
     CDS             4875..5384
                     /gene="purE"
                     /locus_tag="ACHKAY_002499"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO5701166.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5381..6448
                     /gene="purK"
                     /locus_tag="ACHKAY_002500"
     CDS             5381..6448
                     /gene="purK"
                     /locus_tag="ACHKAY_002500"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO5701167.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6585..7478)
                     /gene="ybcF"
                     /locus_tag="ACHKAY_002501"
     CDS             complement(6585..7478)
                     /gene="ybcF"
                     /locus_tag="ACHKAY_002501"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO5701168.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALLRIEETLAGEAGTCISL"
     gene            complement(7475..8290)
                     /gene="ylbF"
                     /locus_tag="ACHKAY_002502"
     CDS             complement(7475..8290)
                     /gene="ylbF"
                     /locus_tag="ACHKAY_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO5701169.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8301..9560)
                     /gene="ylbE"
                     /locus_tag="ACHKAY_002503"
     CDS             complement(8301..9560)
                     /gene="ylbE"
                     /locus_tag="ACHKAY_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO5701170.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9570..11237)
                     /gene="fdrA"
                     /locus_tag="ACHKAY_002504"
     CDS             complement(9570..11237)
                     /gene="fdrA"
                     /locus_tag="ACHKAY_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO5701171.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11553..12602
                     /gene="allD"
                     /locus_tag="ACHKAY_002505"
     CDS             11553..12602
                     /gene="allD"
                     /locus_tag="ACHKAY_002505"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO5701172.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12624..13859
                     /gene="allC"
                     /locus_tag="ACHKAY_002506"
     CDS             12624..13859
                     /gene="allC"
                     /locus_tag="ACHKAY_002506"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO5701173.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13870..14655
                     /gene="allE"
                     /locus_tag="ACHKAY_002507"
     CDS             13870..14655
                     /gene="allE"
                     /locus_tag="ACHKAY_002507"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO5701174.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14784..15929)
                     /gene="glxK"
                     /locus_tag="ACHKAY_002508"
     CDS             complement(14784..15929)
                     /gene="glxK"
                     /locus_tag="ACHKAY_002508"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO5701175.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(15951..17251)
                     /gene="ybbY"
                     /locus_tag="ACHKAY_002509"
                     /pseudo
     CDS             complement(15951..17251)
                     /gene="ybbY"
                     /locus_tag="ACHKAY_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(17308..18669)
                     /gene="allB"
                     /locus_tag="ACHKAY_002510"
     CDS             complement(17308..18669)
                     /gene="allB"
                     /locus_tag="ACHKAY_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO5701176.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18729..20183)
                     /gene="ybbW"
                     /locus_tag="ACHKAY_002511"
     CDS             complement(18729..20183)
                     /gene="ybbW"
                     /locus_tag="ACHKAY_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO5701177.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20352..21230)
                     /gene="glxR"
                     /locus_tag="ACHKAY_002512"
     CDS             complement(20352..21230)
                     /gene="glxR"
                     /locus_tag="ACHKAY_002512"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5701178.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21330..22106)
                     /gene="hyi"
                     /locus_tag="ACHKAY_002513"
     CDS             complement(21330..22106)
                     /gene="hyi"
                     /locus_tag="ACHKAY_002513"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO5701179.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(22119..23900)
                     /gene="gcl"
                     /locus_tag="ACHKAY_002514"
     CDS             complement(22119..23900)
                     /gene="gcl"
                     /locus_tag="ACHKAY_002514"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO5701180.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(23990..24805)
                     /gene="allR"
                     /locus_tag="ACHKAY_002515"
     CDS             complement(23990..24805)
                     /gene="allR"
                     /locus_tag="ACHKAY_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO5701181.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24883..25365)
                     /gene="allA"
                     /locus_tag="ACHKAY_002516"
     CDS             complement(24883..25365)
                     /gene="allA"
                     /locus_tag="ACHKAY_002516"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO5701182.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25595..26521
                     /gene="allS"
                     /locus_tag="ACHKAY_002517"
     CDS             25595..26521
                     /gene="allS"
                     /locus_tag="ACHKAY_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO5701183.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26590..27684
                     /gene="mnmH"
                     /locus_tag="ACHKAY_002518"
     CDS             26590..27684
                     /gene="mnmH"
                     /locus_tag="ACHKAY_002518"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO5701184.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27800..28165
                     /gene="ylbG"
                     /locus_tag="ACHKAY_002519"
                     /pseudo
     CDS             27800..28165
                     /gene="ylbG"
                     /locus_tag="ACHKAY_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28932..29136)
                     /locus_tag="ACHKAY_002520"
                     /pseudo
     CDS             complement(28932..29136)
                     /locus_tag="ACHKAY_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329125.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7221 a         7858 c         7212 g         6845 t
ORIGIN      
        1 tcttaaatta tcttaatcct taaacaagga aataaatcag ttccagattt acaacgccat
       61 catggacgaa aaatgaagct ttcaatctca gcgacctcag cgacggtgcg cctcaccttc
      121 gcaagaggtc gcttcacgcg ataaatctga aacgaaacct gacagcgcgc cccgcttctg
      181 acaaaatagg cgcatcccct tcgacctacg taacagatgg aatcctctct ctgatggcag
      241 caaagattat tgacggtaaa acgattgcgc agcaggtgcg ctctgaagtt gctcaaaaag
      301 ttcaggcgcg tattgcagcc ggactgcggg caccaggact ggccgttgtg ctggtgggta
      361 gtaaccctgc atcgcaaatt tatgtcgcaa gcaaacgcaa ggcttgtgaa gaagtcgggt
      421 tcgtctcccg ctcttatgac ctcccggaaa ccaccagcga agcggagctg ttggagctta
      481 tcgatgcgct gaatgccgac aacaccatcg atggcattct ggttcaactg ccgttaccgg
      541 cgggtattga taacgtcaaa gtgctggaac gtattcatcc ggacaaagac gtggacggtt
      601 tccatcctta taacgtcggt cgtctgtgcc agcgcgcgcc gcgtctgcgt ccctgcaccc
      661 cgcgcggtat cgtcacgctg cttgagcgtt acaacattga taccttcggc ctcaacgccg
      721 tggtgattgg cgcatcgaat atcgtcggtc gtccaatgag catggaactg ttgctcgcag
      781 gttgcaccac taccgtgact caccgtttca ctaaaaatct gcgtcaccac gtagaaaatg
      841 ccgatctgtt gatcgttgcc gtcggcaagc cgggctttat tcctggtgac tggatcaaag
      901 aaggcgcaat tgtgattgat gtcggcatca accgtctgga aaatggcaaa gttgtgggcg
      961 acgtcgtgtt tgaagacgcg gctaaacgcg cctcatacat tacgcctgtt ccgggcggcg
     1021 ttggcccgat gacggttgcc acgctgattg aaaacacgct acaggcgtgc gttgaatatc
     1081 atgatccaca ggatgagtaa catggcgaca ttttctttag gtaaacatcc gcacgttgag
     1141 ctgtgcgact tgctgaaact ggaaggctgg agcgaaagcg gcgcgcaggc gaaaatcgcg
     1201 attgccgaag gccaggtgaa agtcgacggt gcggttgaaa cgcgcaaacg ctgcaaaatc
     1261 gtcgccggtc agacagtgag ttttgcaggt cacagcgtac aggttgttgc ctgatccctt
     1321 tcccggcctg tatcaacagg ccggttctct tttcagactt ttgccctcta cgccccgttc
     1381 atccatgctg atagcatcga cattttgccg aagcgaccat catgccaacc gttattacgc
     1441 acgccgccgt tcccctatgc attggtttag ggttgggttc gaaagtcatc cctccgcgtc
     1501 tgttatttgc cggaatcatc ctggcgatgc tgcccgacgc cgacgtattg tcgtttaaat
     1561 ttggcgttgc ttacggcaat gtttttggtc atcgcgagtt tacccattcg ctggtgtttg
     1621 cgtttgttgt cccgctgtta tgtgtgctga ttggacgccg atggttcagg gctgggctga
     1681 ttcgctgctg gctgttttta accgtctcgt tgctatcgca cagcttgctg gattcggtaa
     1741 ccactggcgg taagggcgtt ggctggctgt ggccgtggtc agatgaacgc tttttcgctc
     1801 cctggcaggt gattaaagtc gcgccgtttg cattgtctcg ttacaccacg ccgtacgggc
     1861 atcaggtgat tatttctgaa ttgatgtggg tgtggttgcc ggggatggtg cttatgggaa
     1921 tgttgtggtg gcgcaggcga taaccggatg cgaaaactcg catccggcaa cagcgcaatt
     1981 acttacgacg ccaggtggtc ccttgcgggc catcttccag cacgatcccc atctcgttaa
     2041 gacggtcacg cgccgcatct gccgccgccc agtctttcgc tttacgggca tccagacgct
     2101 gttgaattaa cgcttcaatc tcagccactt cgctgtcgtc tgcctgcgca ccgctttgca
     2161 ggaacgcttc cggttcttgc tccagcaggc ccaatacagc ggaaagttta cgcaggtgag
     2221 atgccattgc attcgccgct gccatatctt ctgctttcag acggttcact tcacgcgcca
     2281 tatcaaacag cacggaatag gcttccgggg tgttgaaatc gtcgtccatc gcttcaataa
     2341 agcgcgcttc aaacgcttcg ccaccggcag gcgcaacggt tttatctgtg ccgcgcagcg
     2401 caatgtagag gcgctccagc gccgcacgcg cctgcttcag gttctcttcg ctgtagttca
     2461 gttggctgcg atagtggccc gacatcagga agtaacgcac ggtttccgcg tcgtagtatt
     2521 tcagcacatc gcgcacggta aagaagttac ccagcgattt ggacatcttc tcgcggtcaa
     2581 ccatcaccat ccccgagtgc atccagtagt tcacatactg gccatcatgg gcacaggtgg
     2641 actgcgcgat ttcgttctcg tggtgcggga acatcaggtc tgaaccgccg ccgtggatat
     2701 caaagtggtt acccagctgc ttgcagttca ttgccgaaca ttcaatgtgc cagcctggac
     2761 ggcccgcgcc ccacggagac ggccagctcg gttcgccctc tttcgacatc ttccacagaa
     2821 cgaagtccat cgggttgcgt ttgtcgtcga ccacgtcaac gcgcgcacct gcctgcagct
     2881 ggtcgagatc ctgacgcgac agcacgccat atgtcggatc ggtcgggacg tcgaacatca
     2941 cgtcgccgtt gtccgccaca taagcgtgac ctttggcgat cagttgttca gtgagttcaa
     3001 taatttctgc gatatggtgc gtcgcgcgcg gctccatatc cgggcgcaga atgttcaaag
     3061 catcaaaatc tttgtgcatt tcggcgatca tgcgatccac cagcgccaca aagctttcgc
     3121 cattttcatt ggcgcgtttg atgattttgt cgtcgatatc ggtaatgttg cgcacatact
     3181 tcagtttata gccgaggaaa cgcagatagc gcgcaaccac gtcaaaagca acaaaggtac
     3241 gcccgtgacc gatatgacag agatcgtaaa cggtgattcc acacacatac atgccgactt
     3301 ccccggcgtg aataggctta aattcctctt tttggcgtgt cagagtattg aagattttta
     3361 gcatcgaaga ttccgtttag acatgtgtgg gtaattgagt tgcgtataat acccatattt
     3421 cccgcgcgaa tcagcataca ttgcgtgatg atccgatcgt gcggttatgc tataacacca
     3481 ccctatatat gacccgaact gggttgaagc accaatcaaa cggaacagga tgcaaaaatg
     3541 gttactttcc acaccaatca cggcgatatt gtcatcaaaa cttttgacga taaagcacct
     3601 gaaacagtta aaaacttcct ggactactgc cgcgaaggtt tttacaacaa caccattttc
     3661 caccgtgtta tcaacggctt tatgattcag ggcggcggtt ttgaaccggg catgaaacaa
     3721 aaagccacca aagaaccgat caaaaacgaa gccaacaacg gtctgaaaaa tacccgtggt
     3781 acgctggcaa tggcccgtac tcaggctccg cactctgcaa ctgcacagtt cttcatcaac
     3841 gtggttgata acgacttcct gaacttctct ggcgaaagcc tgcaaggttg gggctactgc
     3901 gtgtttgctg aagtggttga aggcatggac gtggtagaca aaatcaaagg tgttgcaacc
     3961 ggtcgtagcg gtatgcacca ggacgtgcca aaagaagacg ttatcattga aagcgtgacc
     4021 gttagcgagt aatcgtggcg acactcttta ttgcagatct tcatctctgc gtggaagaac
     4081 cggcgatcac cgccggtttt ctgcgttttt tagcggggga agcccgcaag gccgacgcgc
     4141 tgtatattct tggcgatctg tttgaagcat ggattggcga cgacgatccc aacccactcc
     4201 atcgccagat ggcggcagcg atcaaagcgg tgtccgattc cggcgttccc tgttatttca
     4261 ttcatggcaa ccgtgatttt ctgctcggca aacgctttgc ccgtgaaagc ggcatgacat
     4321 tattgccgga agaaaaggtg ctcgaacttt atggtcgccg ggtgttgatt atgcatggcg
     4381 acacgctgtg caccgatgac gcgggttatc aggcttttcg cgccaaagtc cacaaaccct
     4441 ggctgcaaat gctattcctc gccctgccgt tgtttgtgcg caaacgcatt gccgcgcgaa
     4501 tgcgagcgaa cagtaaagaa gccaacagca gtaaatcgct ggcgatcatg gacgttaacc
     4561 aaaacgcggt agtcagtgcg atggaaaaac atcaggtgca atggctgatc cacggacata
     4621 cccatcgccc ggcggtacat gaacttatcg ccaaccagca acctgctttt cgcgtggtac
     4681 tcggtgcctg gcatacggaa ggttcgatgg tgaaagtcac ggcggatgac gttgagctga
     4741 ttcattttcc gttttaaaaa acccgcaact ttgctgattt cacagccacg caaccgtttt
     4801 ccttgctctc tttccgtgct attctctgtg ccctctaaag ccgagagttg tgcaccacag
     4861 gagttttaag acgcatgtct tcccgcaata atccggcgcg tgtcgccatc gtgatggggt
     4921 ccaaaagcga ctgggctacc atgcagttcg ccgccgaaat cttcgaaatc ctgaatgtcc
     4981 cgcaccacgt tgaagtggtt tctgctcacc gcacccccga taagctgttc agcttcgccg
     5041 aaagcgccga agagaacggt tatcaggtga ttattgcggg cgcaggcggc gcagcgcacc
     5101 tgcctggtat gattgccgcc aaaacgctgg tgccagtgct gggcgtgcca gtacagagcg
     5161 ccgcactgag cggtgtcgat agcctctact ccatcgtaca aatgccgcgc ggcattccgg
     5221 tgggtacgct ggcgattggt aaagctggcg cggcaaacgc ggcgttactg gcagcacaaa
     5281 ttcttgcgac tcatgataaa gaactgcacc agcgtctgaa tgactggcgc aaagcccaga
     5341 ccgacgaagt gctggaaaac ccggacccgc gaggtgcggc atgaaacagg tttgcgtcct
     5401 cggtaacggg cagttaggcc gtatgctgcg tcaggcaggt gaaccgttag gcattgctgt
     5461 ctggccggtc gggctggacg ctgaaccggc ggcggtgcct tttcaacaaa gcgtgattac
     5521 cgctgagatc gaacgctggc cggaaaccgc attaacccgc gagctggcgc gtcatccggc
     5581 ctttgtgaac cgcgatgtgt tcccgattat tgccgaccgt ctgactcaga agcagctttt
     5641 cgataagctc cacctgccga ccgcaccgtg gcagttactt gccgatcgca gcgagtggcc
     5701 tgcggtgttt gagcgtttag gtgaactggc gattgttaag cgtcgcactg gtggctatga
     5761 cggtcgcggt caatggcgtt tacgtgccaa tgaaaccgaa cagttaccgg cagagtgtta
     5821 cggcgaatgt attgtcgagc agggcattaa cttctctggt gaagtgtcgc tggttggcgc
     5881 acgcggattt gatggcagca ccgtgtttta tccgctgacg cataacctgc atcaggacgg
     5941 tattttgcgc accagcgtcg cttttccgca ggccaatgcg cagcagcaag cgcaagccga
     6001 agagatgctg tcggcgatta tgcaggagct gggctatgtg ggcgtgatgg cgatggagtg
     6061 ttttgtcacc ccgcaaggtc tgctgatcaa cgaactggca ccgcgtgtgc ataacagcgg
     6121 tcactggaca caaaacggtg ccagcatcag ccagtttgag ctgcatctgc gggcgattac
     6181 cgatctgccg ttaccgcaac cggtagtgaa tagtccgtcg gtgatgatca acctgattgg
     6241 tagcgatgtg aattatgact ggctgaaact gccgctggtg catctgcact ggtacgacaa
     6301 agaagtccgt ccggggcgta aagtggggca tctgaatttg accgacagcg acacatcgcg
     6361 tctgaccgcg acgctggaag ccttgatccc gctgctgccg ccggagtatg ccagcggcgt
     6421 gatgtgggcg cagagtaagt tcagttaagc aggtgctcat ttgattgccg gatgcggcgt
     6481 aaacgcctta tccggcttac cgctccggta cccattgtag gcctgataag acgcgtcaag
     6541 cgtcgcatca ggcatggtgc accgattgcc ggatgctctc atcactacag cgaaatacag
     6601 gtccccgctt cgcccgccag cgtctcttca attcgcaata acgccccaat ccacgcgggt
     6661 ttaccacggc ttctgacata accacttacc gccgttacct tcggccccat cgaaccatcg
     6721 gctttggtaa atggcgctaa ctcatccggt gtggcatggc gaatggcgcg ttgctgcggc
     6781 gttccccagt tttcataaac cgcgtcagca tcggtgagga tcaccagtcc atcggcatta
     6841 atctgctcgg cgagcaacgc agcggcgaga tctttatcaa tcaccgcttc actccctgcc
     6901 ccatcttccg tcacaggcac accgccgccg ccactgcaaa tcaccacatg cccctctttg
     6961 agcaacaact cgatggcttc gctgtcgaga attttacgcg gttgcggaga cgccaccacc
     7021 cggcgcaaat atttaccatc acgtttcatc tgccagccgt aagccgcttc cagtgccttt
     7081 tgttcttctg gctgataaac cggaccaata aatttctctg gctgcaaaaa cgccggatca
     7141 tcaggcgaaa cctcaatgcg cgtcagcacc gtcgtgacag gcggcatctg cggctgtgcg
     7201 ctcaaactct gcgccagcat atagccaatc atcccctggc tttccgccac cagcacatcc
     7261 agcggatacg gatctacctc tttccacgcc agattctgca atgccagcag ccccacctgc
     7321 ggcccattgc cgtgaacaat cgccagccga taagaacggg ccaggcgcgc cagcgcgggt
     7381 acagcactgg cgatattgcg atattgattt tctgccgtca gcgcctcacc gcgctggagt
     7441 aaggcgttgc ccccaagagc tacaaccagt gttttcatgg ttttccttgt aataattgtt
     7501 ggccaagcca gaaccccagc agcgtatctg cccctgacgt atgccccagc gccagcagcg
     7561 aatcaatcgc aacagcggta cgcttcgggc aactcagagc atggacaaag tgcaggagtg
     7621 gcgaggcgaa atatccttgc gcggcataac gtaaataact gacgctcacc gctgtggtaa
     7681 cgagttgaag attgtcggaa caggcaaaaa acgggcgacc ggcgcgcgca tctaaagcgc
     7741 cataatacca ggccgccagc agcattccgc tcaacgtgtc atcatgactt ggcgttaatc
     7801 cggggccttt acccagccag tgccgccagt cggtcttaac gccattgagc gcggcctgaa
     7861 aacagtgacg aaactggcgt aattcagcgg gcagcggatc gcttgccgcc atcgccagtg
     7921 gtccgaaaag tccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat
     7981 gcgaaggcgg cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc
     8041 cgaggcgaat cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata
     8101 acgcatcgaa ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac
     8161 gatgcagcgt taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc
     8221 ccgctaaccg ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga
     8281 tgatcgtcat tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc
     8341 tgttcaaaac acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc
     8401 tctttatggg cgataccggt attgatgagt ggcgtaatgc cggtttctac cacgcgacga
     8461 atatccagcc ccaggcacgc gccctggaaa tcccagccgg ggatctgcaa ctgcatattg
     8521 cgctcaaggt aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc
     8581 gcaccgacaa agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag
     8641 gtttcggtaa tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg
     8701 aaaaacagac cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt
     8761 acccgaatcc cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg
     8821 atcatcgcgc cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac
     8881 tgatcggtca cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg
     8941 cgagcaattt gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga
     9001 tggaactcat cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc
     9061 tccatgcgcc ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg
     9121 cgagtcagga catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg
     9181 aggttgcagt acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg
     9241 gcagaagtaa taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc
     9301 acttccccct gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac
     9361 agacatgcgc ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc
     9421 ggcccggcgt gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt
     9481 tgcacatcca gccagtgtgg acgagcacga cgaatttgtt cgataaccgc agcattggct
     9541 tgcgccactg atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagt
     9601 tttttattgc cgccggcgac tggcgaccat tggtaatgca caaccggttt gctggcgctt
     9661 tgtagctcca gcgcaaagct gcgtaatccg atgttaatca cggcgacgtt ttccagtaat
     9721 gacggtgtgt gttgctgtgt ggcagacgag agcggatgaa ttaacgctgc cgccagcaag
     9781 gtggcttccg gtagcgaact cactaccgcg atccccgcat cttccagcgt ggcgatttgc
     9841 tgcgagcggc attgcgggtc acgttcggta cctgtcaccg tggcaatggc gtacagcggt
     9901 tggctgtctg aacgcgcggc acaggctttt tgccaggcgc tcaccagcga ggcagcagga
     9961 tcggcggtcg caccgaagcc aatcacgaca tcaagcagca acacgcgcac ttgcggttta
    10021 gcgccgagat cggcaattaa ctggttgcgt aaggctgggt cgatcatcgg atggggacgc
    10081 ccgacggtgt agaaatcatc gcccagatca atgatctggt gaccatcggc gtccagcatc
    10141 atgccgtgat gatgggtatc gtcggcttcc acgccaaggt gcccggcaag taatcccgcc
    10201 gcttcggcag caagcgtacc gccggtatac aaaccgcaaa taaatccgct gctgacaggc
    10261 gctattgcgt tacgtcgcgc cgtgacgcgt gaaagcaggc aagccaggcg tgcggcctca
    10321 tccagcgagg aggcaaacca gacattctcg tcgcgggcca ccgccggggt ataacctaaa
    10381 aacagcgcca ccgtcggttt gccggttgct ttcatggcat taacaatttt cagacgcaca
    10441 gcttcggcag gtggttttga aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc
    10501 atttccagcg ctgttagcgc actgatgccg cccacttcac ggctgaggtc gcgcccgcca
    10561 aggccaatcg cgtgagtaat tccctcccct gccagcgcaa tctgcgaaca cagctcctga
    10621 atcccggtac cggaagcgcc aatgacgcca atattgcctt ctggcatcac gttagcaaaa
    10681 gccagcggtg tgccggcaat catcgacgta ccgcagtccg gccccatcac cagcaagcct
    10741 ttttcccgcg cgcgggtttt aagttggatt tcatcttcca gcgtgacgtt atcggagaac
    10801 atcatcacgt tgaggttgcg atccagcgcc tggtttgtca gctccgccgc atactcgcca
    10861 gccactgaaa tcagcgccag attggcatcg ggtaatttct gacaggcgct gtcccagcga
    10921 cgcacctgcg tcaacgcctg gctgctgcct gacccctgcg ccagttgttt tagcgcctct
    10981 tcaagctgct gcataatcgc ctgcgcgatc cccgcatccg ccgcttcgct acgaattgcc
    11041 acgcaaatat cgttcggcgt ggcgttatta aaatcgtcat gccagaaacc tgtggtatct
    11101 aataacgctt tattggcggg cgtacccatc attacggaaa catcatcaac attttctgat
    11161 tcgctgagtt ttcgtgaaat aatcattaaa ctgaccgaat cctgaaaaca ccctttttta
    11221 ataaaggcgt ggatcataac aactccttaa aaattgcacc catggcaatt gcacgagcag
    11281 tgttaatgtg gcgattacgg tatcgatgac aacgagtaaa taaggtgatg aatatcacac
    11341 agggattatt aacaaaagaa ttcaaataac cgccaggcta ttacgcatta ttttttaata
    11401 agcgtggttg ttttaaaaat caggttaaaa gcgcgaagat tatcaaataa attctaacta
    11461 tattttttgc ctgtctggat cacataatcc agatattttc cctgtatgtt aattgcagtc
    11521 atgcttcaca ccgtcgttaa aaaggaagac agatgaaaat cagtcgggaa acactccacc
    11581 agctaattga gaataaactc tgccaggctg ggttaaaacg tgagcacgct gcaaccgtgg
    11641 ctgaagtatt ggtttacgcc gatgccagag ggatccactc tcatggcgcg gtgcgcgtgg
    11701 aatactacgc ggaacgcatt tcaaaaggcg gcaccaaccg cgaaccggag tttcgtcttg
    11761 aggaaaccgg gccgtgctcg gcaattttac atgccgacaa tgccgccgga caggtcgcgg
    11821 cgaaaatggg tatggaacat gccatcaaaa ccgcccagca aaatggcgtt gcggtggtcg
    11881 gtatcagccg gatgggtcac agcggcgcaa tctcttattt tgtgcagcag gcagcccgcg
    11941 ccggattaat tggtatttcg atgtgccagt ccgatccaat ggtggtgccg tttggcggcg
    12001 cggaaattta ctacggtact aacccactgg cctttgccgc gccgggagaa ggcgacgaga
    12061 tccttacctt tgatatggcg actaccgtac aggcatgggg aaaagtgctc gacgcccgct
    12121 cgcgtaatat gtctatcccg gatacctggg cggtcgataa aaacggtgca ccaacaaccg
    12181 atccgttcgc ggtacatgct ctgctccccg ccgccgggcc aaaagggtat ggcctgatga
    12241 tgatgattga cgtcctctca ggcgtcttac tcggcttacc gttcgggcga caggttagtt
    12301 cgatgtatga cgatttacac gccgggcgta atttggggca attacatatc gttattaacc
    12361 cgaacttttt cttctccagc aaattatttc gtcaacatct tagccagacc atgcgcgaat
    12421 taaatgccat tacccccgcg cccggtttta atcaggttta ttatcccgga caggatcagg
    12481 atattaaaca acgcaaagcc gccgtcgaag gcatcgaaat tgttgatgat atttaccagt
    12541 atttaatttc cgacgcgctt tataacacgt catacgaaac gaaaaatccc tttgcgcaat
    12601 aattattgag acaggacttt cttatgatta cacattttcg tcaagccata gaagaaacgc
    12661 tgccctggct ttcctctttt ggcgctgacc caacgggtgg tatgacccgt ttactttatt
    12721 cgccggaatg gctggaaact cagcagcaat ttaaaaaaag aatggcagca agcgggctgg
    12781 aaacacgttt cgatgaagtg gggaatttat atggtcgcct gagtggcacc gaatatccac
    12841 aagaagtggt tctgagcggt tcgcatatcg ataccgtggt taacggcggt aacctcgacg
    12901 ggcaattcgg cgcgctggcg gcgtggctgg caattgactg gctgaaaacg caatacggcg
    12961 cgccgctacg tacggtcgaa gtggtggcga tggcagaaga agaaggcagc cgcttcccgt
    13021 atgtcttctg gggcagtaaa aatatctttg ggctggcgaa tcctgacgac gtgcggaata
    13081 tctgtgatgc caaaggaaat agttttgtcg atgcgatgaa ggcttgcgga tttactcttc
    13141 cgaacgcccc actaactccg cgtcaggata ttaaagcctt tgttgaactg catattgaac
    13201 agggctgtgt gctggaaagt aatgggcaat caattggcgt ggtgaatgca attgtcgggc
    13261 agcgtcgtta tacggtaacg ctgaacggcg aatcaaacca tgcaggcacc acgccgatgg
    13321 gttatcgtcg tgatacagtt tacgctttca gtcgcatttg ccatcagtcg gtcgaaaaag
    13381 cgaaaaggat gggcgatccg ctggttctga cctttggcaa agtagagccg cgcccgaata
    13441 cggtaaatgt ggtgccgggt aaaaccacgt tcaccattga ttgtcgtcat accgacgctg
    13501 ccgtgctgcg cgatttcacc caacagttag aaaacgacat gcgggcgatt tgcgatgaaa
    13561 tggacattgg tattgatatc gatttatgga tggacgaaga acccgtgccg atgaataagg
    13621 agctggtcgc caccctgaca gaattgtgtg aaagagaaaa actgaattac cgggtgatgc
    13681 acagtggtgc cgggcacgac gcgcaaattt tcgcgcctcg cgtaccaacc tgcatgattt
    13741 tcattcccag catcaacggg atcagccata acccggcgga acgcaccaat attaccgacc
    13801 ttgccgaagg ggtcaaaacg ttggcactca tgctttatca acttgcctgg cagaaataag
    13861 gagtcataaa tgggatattt aaataacgtc accggttacc gcgatgattt actggctaac
    13921 cgtgcgattg ttaaacacgg taatttcgca ctgttaaccc cggatggtct ggtaaaaaat
    13981 attattccgg gctttgaaaa ttgtgacgcg acaatcctct ccacgccaaa gctgggtgcc
    14041 tcttttgttg attatctggt cacactgcat caaaacggtg gcaaccaaca gggcttcggt
    14101 ggcgaaggca ttgaaacgtt cctgtatgtg atctctggaa atatcactgc caaagccgaa
    14161 ggcaaaacat ttgccttaag cgaaggtggc tatctttatt gcccaccagg ctccttaatg
    14221 acgtttgtta acgcccaggc cgaagacagc caaatctttt tatataagcg ccgctatgtt
    14281 ccggtagaag gccatgcacc gtggctggtt tctggtaatg ccagcgaact ggaacgcatt
    14341 cactatgaag gtatggatga tgtgatcctg ctggatttcc tgcccaaaga gttaggtttt
    14401 gatatgaaca tgcatatcct ctcttttgcg ccaggtgcca gccacggtta tatcgaaaca
    14461 cacgttcagg aacatggtgc ctatattctt tccggtcagg gggtttataa cctcgacaat
    14521 aactggatcc cggtgaaaaa aggcgattac atctttatgg gcgcttattc tttacaggct
    14581 ggttatggcg tagggcgtgg tgaagcgttc agctatattt actcgaaaga ttgcaaccgc
    14641 gacgtagaga tttaagtcat accactcgcc tgattatttt tagctatgtt ggattttgcc
    14701 acggtttata ccgtggcttt ttttgtagta aattcaggta aaaaaagatg cgaaataaat
    14761 cgcatccgct ttaaaggtaa gggtcagttt ttaattccct gacctatttt aatggcgcag
    14821 gcaatatttc gcgcgctgtt gaagagattg gtttcaccgc tggcgagcac ttcggctaaa
    14881 ggtgccagac gaggcaaaat gctgaatacc gcgtcaatgc cgtactggtg caccacttcc
    14941 acgccatcac ccaatacgcc agcaatccca atcaccggga cattaaactg cttcgccacc
    15001 gacgcaacgc ccagcggcgc tttaccgcct gccgtttgcg agtcaatgcg cccttccccg
    15061 gtgatcacca gtgctgcgcc ctgcaccgcc tgctccaggt tgaccgcccg cagtacaatc
    15121 tcaatacccg gtttaatatc cgcattgagg aataccgccg ccgcaatacc cataccgccc
    15181 gcagcgccgc cgcccgccat ctggcagaca ttaatttcag tttgctgttg taaaacacgg
    15241 gcgtaatttt gcagcccctg ttcaagttct tcgaccatct ccggcgttgc ccctttttgc
    15301 gggccaaaca ccgccgccgc gccgcgtgcc cctaccagcg ggttatcaac gtcacatgct
    15361 acttcaatat ggcaattcgc cagacgcgga tcgcattcat ccatctcaat gcttgccacg
    15421 cgcgctaaat taccgccgtt agccgccagc acctggccgt cggcatcaag gaaacgcaca
    15481 ccaagcgcct gagccatgcc cataccgccg tcgaccgtcg cactgccgcc aatgccgaga
    15541 ataatatggc gaatgccgtt atccagcgca tgacgaatta actcccccgt accaaaactg
    15601 gaggccagca acggattacg cttttcaggc gcgaccagca tcaggccact tgctgccgcc
    15661 atctcaatca ccgccgtttt cccgtcgccg gtaaggccat aaaaagcatt cactgtttcg
    15721 cccatcggcc cgcagacttc aagcgtcacg atgttgccgc ccgtcgcggc gaccatcgca
    15781 tccaccgtcc cttcgccgcc atccgctatc ggcaaacaga tatagttcgc atcgggaaag
    15841 agggtcgaaa acccggcttt aattgcctga caacattttt ctgcacttaa gctctcttta
    15901 aaagagtccg gcgcaatgac aatcttcatt atcaatcctt tttcaacaaa ttattcgatg
    15961 cgttcccacg gaataagatt atccataaga acagccagta aaattccgac cagtaagccg
    16021 ttactgagca gaggacgcag cgttaatgga aggtcttgca gatacacagg cggtaatgcc
    16081 attaaaaata tgccgacaaa taacggcaat gcgagtcgat aaatattgcg agcggtaaag
    16141 gttatttgct ggctaaacac cagcgcggaa aagagtaaag gcagataaga aaccagcatg
    16201 accgcgctac tcacgggtaa agggatactg caaaacagtc gtgtgagtgc cggaaccagc
    16261 gccaccagca ggcaaataac gctgccataa ataaacgaac gccgcgtgta atcgcctgtc
    16321 tgggttaata aaccaatgga tgaaacaaac ggtgaaaatg gaattaccgc cagcggtacg
    16381 gttatcagcg tcataaatcc ggtcgccaca aagctgcgac gataacgcgt attcccgcgc
    16441 cctgctgcgg ataaaaaaca tccgtgcccc gaatcgcacc gtaggtattg ctgatattca
    16501 ccagacctgt aatcaccgcc gtcagaataa ttcccggaga caaagcacct ccactgccga
    16561 gcgggaacca ctgccagtgc aactcaccgg agagcgagtg cgaagaagga aagcaaaagt
    16621 accacaacaa ccagccggtt atggtgccga ccagcaggcc ataacgggca aaacgttgcg
    16681 gcaggaagat aatcatcccc agtaccaggc acatcaccgc caccgagagc gcgaacggcg
    16741 gtaactgaat tttaaaattt gggtcggcta tgccaaacgg cagcccgagc atacctttga
    16801 aaaagatagt ggtcagctgc gcgcccagca tcaacataaa caagaccatc accgacggcg
    16861 taaataaccg tgccaggcga tggcctaatc cgctaaaacc aatcaacatc gtcagcacgc
    16921 cggagagcgc aatccccacc gccaggctgg tagcgatatc gttgatcggt gtcccgcgcg
    16981 atgcttcgcc aagggtgata gtaaggatgg ttccccacca caggccaccc ggctcttcca
    17041 taatagcgcg acgatgaccg cagaacgcct gagcgaagca ggccagtgcg gtagcaagaa
    17101 aagcatattg cgtgagcgta agcaggctac tttgcggcaa ctgaaaagca gaaagtagcg
    17161 taggaggaac cacaaccgtg ttgcaaaaaa taaagaaaaa ccactgaaat cctgataaca
    17221 ggctttcgcg gctcactgca aaattgaaca taaacacacc ttaaccggag aatgcccgcc
    17281 gcaaggacgg gcattgcagg ggccagatta ctgctgatgt ttaaggataa attgaccttt
    17341 cggcgcaaca gggaagccct gttcaatgtc gtaaatcaca tcaccacgta agatggtttt
    17401 cgtgatacgc gcgccaatgg tacggccaac atacgggctg actttgtggc gatattccag
    17461 atcgtcattg gtaagaacat agctgctatt cggctgaatg aagacgaagt cggcatcttt
    17521 tcctggggcg atacggcctt tttgctgcag accgaaaata tctgctgcgt tagtcgccat
    17581 taatttgccg aacattggca gagacattcc gcgtttctgt accgcttcat cgaacatcac
    17641 gtccatacag ttttgcagac cggcaatacc gccccatgct tccatgatgt tgccggcttt
    17701 catttccgga gggcatggtg agtggtcgga aaccaggcag tcgatttcac cgttaaacag
    17761 tttttcccac atgcctttct ggttttccag atcgcggatc ggcggtgaac acttcgccag
    17821 agtgccgatt tcttcgaact gatcggtatc cagtacaaag taatgcgggc aggattcaca
    17881 agtaacgtcc tgaccttcct gacgtgcacg agtcacttct tcaacgcctt ccgggctgct
    17941 gatatggcaa acgtgcagac ggcaaccggc aactttcgcc agatacagca cgcggcgaat
    18001 cgcttccact tcggtaaata ccggacgcga agccacatag tcatgggcag ttacgcgacc
    18061 ttcacgtttc gcttcttcgc ccagtgcgtc acagatcagc gcgttttcgc agtgcaccag
    18121 caccggctgc cccagttcgc ccagcttctg cgcacctttg aagaactgcc agtcgtttac
    18181 atcacggaag tcgttgtcga taccgcgatc gccacaggtc gcaacgaagc atttgaagcc
    18241 gacaacgccc acttcatcca gctcatgcag acggtcgatg ttgtaagaca ccaggccacc
    18301 gagttgtgcc gcatcgatag tcagcttgcc tttagcggca tcgaacttca gttcaattga
    18361 agcgcggtca accgttgcag gcagctggtt gagcggcatt tcgatcatgg tggtgatacc
    18421 accttttgcc gctgcgcgag taccggtttc ataaccttcc cagtggctac gacccggttc
    18481 agaaatatgg gtgtgcgcat caaccatgcc cggcgaaacc accagaccag acgcatccat
    18541 aacttctttt gcatcgccca gatcctgacc gatagcagca attttcccgc ctttaacggc
    18601 gatatctaca acgcgagctt cgttttctaa aataacggtg ccgtttttaa tgattaaatc
    18661 aaaagacata acaaactcct tgtaaaatac agattggttt ttaactcaat gttcaatatc
    18721 gggattaatt aacctatggt tttttgctct cctgtttttt ctgctgttgt acgtttcttt
    18781 aataaggcgt aggccgcaaa ggcgacgatg acgccgacaa accatgaaac acgcgataac
    18841 ggttccataa agtgaataaa cttaccgcca agagataaaa taacggccac cagagttact
    18901 gaaaacgcag tgaggttaaa accgttatcg taatatttat aatcgccagg tgcggtatac
    18961 agttcatcaa gattaatttg tccgcgcatc accacaaaat aatgcgccat catgacacca
    19021 attaccggac caagcattcc gccgataata tcgaggaaaa gataaatgct gtcctgattt
    19081 tccattaatt tccacgggca gatcagcaag ctgataatac tggcaatcag tacgccgttt
    19141 ttataggtca gttttgtcgg tgcaatggcg gcaatctgat aaccggctgg aataatatta
    19201 ccggtcgcgt tagtggagat agttgtcatc agaataacca gtaccgcaaa gaacgaggcg
    19261 aacaggctgt cccaacgctg aacaatatcc agcacgttcc aggtatcagc gccgtagtga
    19321 atactggctc cggcaataat acataccccg gcgaccgcaa acagaatata ggccacaact
    19381 aaacccagcg tttgccccag cgcctgctca cgaaacgagt gggcgttttg cgtaaagtcg
    19441 gatgcgctca ccgccggtgc cgcccagacc gcaactaccg cgttaatcac caccaggaac
    19501 aggaagccac cgttttctgc tttctgaata ccgctcggaa tgtagtcaaa gattggaccg
    19561 atcccgacca gtgaaatcgc ccaaatcgcc ataccgccga aaacgatata gatgcacggg
    19621 ttaagaatgg cagtgaattt atttaaaact ttgccaccgc caaaacctat accaacgttg
    19681 accagccaga agattaagaa agtaattaag cccggtagag aaaggcctaa cagagtgaaa
    19741 tcaccaccga gagttaaaaa tcccggccag attttgccaa tcagaatcaa gcaggccagt
    19801 gaccccgcgt aacattgcaa accaaaccac atgatggcgg caattccgcc ccttaataat
    19861 ccgggaaaca gtgcaccacg tacaccgtaa gaagcacgca ggatcatggc aaaaggcaca
    19921 ccgtatttac tgcccgcagc accgtttaat accattaccg cggcaatgaa aaaggcgctg
    19981 aggataattg ccagcataat actaaaggta gacaagccga gaataaaaaa gccgccgacc
    20041 atcacataat tgggaacgtt atgaaccgaa cccatccata aggtaaaata gttaaatgtt
    20101 ttccaggtcc gctggctttg cgttttcggc aatagatctt cgctatagcc gcgttgctgg
    20161 aatagttttc tctgatgttc cataaatccc tcttaatttc acgtatcgaa taacgttgtt
    20221 ttaatctagc tatgaggaac aaataaaata aaaatattat ttttaatact gaagcaacat
    20281 ctttggctgc tttctttttt aatattgctg gccgattcat tttctgatat cggccatttt
    20341 tattgcgggt atcaggccag tttatggtta gccattaatt ccagcgcctg cactaacgca
    20401 gagtgatcca actggctgcc accgtttgcc gcgcaggtat taaataactc ctggcaggtc
    20461 gcagtgtttg gcaggttcag cgctagcgct ttcgcacttt gcagtgccag gttgagatct
    20521 ttctggtgca gcgcaatttt gaaacctgga ttaaaagtgc gtttaatcat gcgctcaccg
    20581 tgaacttcca gaatacgtga ggaagcaaag ccgcccatca gcgcctggcg cacacgtacc
    20641 gggtccgcac cggcttttga agcaaatagc agggcttcag aaaccgcttc aatattgagc
    20701 gccacaataa tctgatttgc cactttgcag gtttgaccat cgccgttacc gcccacgagg
    20761 gtgatatttt taccgagcag ttcaaacagc ggtttaacac gttcaaatac cgcttcatca
    20821 ccgccaacca taatcgacaa cgtcccttca cgcgcaccga tttcaccgcc ggagactggc
    20881 gcatcgagat aatcgccgcc cagttcattc acctgacgag cgaaacgctt agtttcaatc
    20941 ggggaaatgg agctcatatc aacaatggtt ttgcccttca gcgaggcttt ggtacaacca
    21001 ttttcaccga acagaacttc ttcaacctgt ggtgtatctg gcaccataat aaaaatgatg
    21061 tccgatgctt ccgttacctg gcgcgcagtt tcaacactga cggcacccag tgacagtaat
    21121 tcatcagcaa ccggaccaat ggttgtgaca tgcagttgat gaccggcacg cgccagatta
    21181 atggccatcg gtgtacccat aatgcctaag ccaataaatc ccagtttcat aattaacctc
    21241 ttttaaattt cgcttttcct gaattcagac aacacgatcc cctgccaact tagcggccta
    21301 aaaaagcatt gcctgaacag cgttacgttt taacggtacg gatccatcca gcgtaaaccg
    21361 gcttccgtgg tggtttgggg tgtatattca cacccaaccc aaccgttgta gtcagaattt
    21421 tcgattacct taaagagata atcataatta atttctccgg tccccggttc gccgcgatgc
    21481 ggattatcag caatttgcag gtgaccaatt ttatcggccc actgagtcat ggtgttggtt
    21541 aattcacctt ccatccgctg catgtgataa atgtcatact gaattttcaa attgcagcaa
    21601 ccaacatcat caatcaattt cagcgcctgc cgagttccgg tgagatggaa accaggaata
    21661 tcaaaatggt taataggttc aatcagtaat aaaatatctt ctttcatcag catattcgcg
    21721 gcataacgca ggttttctac aagcgttgcg tgaatctgtt cactgctgaa accagccggc
    21781 gttttaccga ccagacagtt aattttttta ttacccagcg cacgggcata acgaatcgct
    21841 gctgctacgc catcccgaaa ctcttcttca cggccaggaa tacaggcaat accgcgctcc
    21901 cccgccgccc agtcacctgc cggtaaattg tgcagcgtat gttcgagttt attactcgcc
    21961 agcacctgtt ttaattcttc aatgtcgtag tcataaggaa acataaattc aacgccgcga
    22021 aaaccacact gcgcagcttt ctcaaaacgg gcgagaaaat catattcacc aaataacatc
    22081 gataaattag cagagaaacg taacattatt tatctccctt attcatagtg catgaagcag
    22141 gtttcagtcg gtgcgtccgc tgcgttatcg gcgatatctt caaattccat gacgttatcc
    22201 agttcgctgc ccatcgaaat attggtcaca cgctcaagaa taacttccac gactaccggt
    22261 acccgatatt gcgccattaa ggctttcgcc tgttcaaagg ctggcgcaat atcttccggt
    22321 ttgaagaccc gaatagcttt acaacctaaa ccttccgcta cttttacgtg gtcgacgccg
    22381 taaccgttca cttcactgga gttaatattt tcgaacgcga gctgtacgca gtaatccatg
    22441 tcaaaggcgc gttgtgactg acgaatcagc cccagataag cgttattgac cagtacatgg
    22501 atgtacggaa tgttgaactg tgcgccaacg gctaattctt caatcaggaa ctggaagtcg
    22561 aagtcaccgg aaatcgccac cacattccgt tctggatcag cagcacacac gcccagcgcc
    22621 gccggaatgg tccagcccag cgggcctgcc tgaccacagt tgatccagtg acggtcttta
    22681 aagacatgca gcatttgtgc cgcagcgatt tgtgacaggc caatggtggt gacataacaa
    22741 acatcgcgac caaaggcttt gttcatctct tcatacacgc gctgcggttt caccggcacg
    22801 ttgtcgaagt gggttttgcg cagcaaagtg cgtttgcgct gctggcagtc ggcgacccat
    22861 tctttacgac acggcagacg acccgctttt tgcatctcct gcgccacttc aaccagcagt
    22921 gtcagcgccg ctttagcatc agagacaatg ccgagatccg gacacagcac gcgaccaatt
    22981 tgcgtcggct caatatcgat atgaacgatt ttgcgccctt cggtgtattt ctctaccgaa
    23041 ccggtatgac ggttagcaaa acggttaccg ataccaaaca ccatgtcgga cgccagcagc
    23101 gttgcgttac cgtaacgatg cgcggtttgc agacccacca tcccggccat cagttcatga
    23161 tcgtccggga tacagcccca gcccatcagc gttgggatca ccggaacgct ggtcagttca
    23221 gcaaactgtt gtaacagtgc agctgcgtca gcattaatta ccccgccccc ggcaacaatc
    23281 accggacgtt cggcctggat taacatttct acagcttttt cgatctgcat acggctggca
    23341 gcaggtttgt agaccggcag cggttcgtac atatcaggat caaactcgat ttccgcaacc
    23401 tgaacgtcga acggtaaatc caccagtacc ggacccggac gaccagaacg catcaggtga
    23461 aatgcctgtt gcaacacacg aggcaccagc gccgcttcac gtactgtaac cgccatttta
    23521 cttaccggct tggcgatagc ttcgatatca acagcctgga aatcttcttt atgcagacgg
    23581 gcgcgcggag cctggccagt aatacacaga ataggaatgg aatcagcaga agcagaatag
    23641 agcgcggtga tcatgtccgt gcccgcagga ccggaagtcc ccagacatac gccgatattc
    23701 cctgccgttg cgcgggtata accttccgcc atgtgcgaag caccttccac atgacgcgcc
    23761 agaatgtgac gaataccgcc gtgcttacgc atcgctgagt agaacggatt gattgcagct
    23821 cccggaacgc cgaaggcggt ggtgatacct tctttctcca gcacatacat tgccgcgtca
    23881 acggctctca tttttgccat tttattccaa cctctattaa ttggaaaaat tttccaactt
    23941 tcaaattttg tcaaaaaaac ccgctccgca gagcgggatg cgacagacac tatggatgtg
    24001 ctttcagtcc caacgccgtg ctgatatcgc gggcggtgtc tctgaccagc tcaccctgac
    24061 tgacaaaacg atcttctgtc agtcttgatg aaggcccgga gatggagata gcggcaacaa
    24121 cgctaccgac atcatcgtaa attgctgaag ctatgcaatt cagacctaca acatgctctt
    24181 ctttatctac ggtatagccc agttcacgcg cttgttccag gtccttcagc aaggtgggca
    24241 tatccacaag cgtagttggc gtaaactgct gcaaaccggt ttgcagaatg atgctcatca
    24301 actcctcttc cgccagcgga taaagcaacg ccttgcccgc accggaagca tgcagcggca
    24361 gacgactgcc cagtggcgca cacatcctga ccatcgattt acactctaac tgaccaatta
    24421 ataccgcttc attgccgtta cggatcgcga cattgaccgt ttcgccggaa agtaacatca
    24481 ggcggcgcat aaacggcccg gcgacggaga ggacatcgcg gttatggatg tacgccgcac
    24541 cgacgttaaa gacacctaat cctatatgcc accagcctaa ctgactgtcc tgatagacaa
    24601 aatccgctgc ctgtaaaacc ttcagcaagc gaaaggtcgt agagagcggc aaatccagat
    24661 tgagagaaat atcgctaacc gacgaacttc ccccactttt ttccagatat tgcagaatcg
    24721 caattccccg ctctaacgcc tgtgcgccct tctgtgccac aggctccgcc tgtcctggcc
    24781 tgccgcgccg tctaacttcc gtcataccta cgttaactcc gacctacaag tgagcataaa
    24841 caatgaacaa ttagtttatc ttatgcaagt cggttgcagg cgtcacgcaa aacagagttc
    24901 ctgttcagga atactttcaa catcacagtt gtcactgccg ccgcgatcga tggtcagaaa
    24961 atcggtgacg cgctgccagg cgaaaagtgg gtgatgccag acgttacggt ggtaattcac
    25021 tccctgttcg ccgttggtaa taaacgcccg cagggttgac aggtctggtt tgtcgtcacc
    25081 taacgccacg accaccacaa acacctcacc tttcatcggg ataaaggcct gagtacccag
    25141 cggatgacgt tcgagttcgt gaatggtcag cggcagattc gccggttgcg cgcggttaat
    25201 gctgataagc gtacggtctt gctcaagaat ctcaaccagc gccaaatcgt ggtaacgctc
    25261 caccaggcca ttgttaatat ggaaaaaatc ccgttgctgc gtttcgatta cgtcgccata
    25321 agcactaaag gcttcctgac ttaacggtaa tacctgaagt ttcatctttt tgtccttgta
    25381 acataattcc gttcaaccac cgccgaccgg tcagcttggt ttcaataaca ggcaatctaa
    25441 aaccttaaga gaacaacatc aatacaaaag ttggaatatt tttccaaaac tagatttagt
    25501 ccacaaattg aacagggtta tcatgtaaaa atactgataa atcagaggat aagaaaatca
    25561 cgcaacttgc ggatttaaaa ggggatacct gaagatgttc gatccagaaa ccttgcggac
    25621 tttcattgcg gttgctgaaa caggaagttt ttcaaaagcg gcagaacgat tatgtaaaac
    25681 cacggcgacg atcagttatc gcattaaact tctggaagag aataccggag tagcgctgtt
    25741 tttccgcacg actcgcagcg tgacgttgac agcggctggc gagcatctac tttcccaggc
    25801 cagagactgg ctgagctggc tggaaagtat gccaagcgag ctgcaacagg tgaatgatgg
    25861 cgtggaacgc caggtgaata ttgtcatcaa caacctgctc tacaaccccc aggccgtcgc
    25921 ccagttgctg gcgtggctga atgaacgtta cccctttacc cagtttcaca tctcccgaca
    25981 aatctatatg ggcgtctggg attcgctatt atacgaaggt ttttcactgg ctatcggcgt
    26041 cacgggaacc gaggcgctgg caaatacctt tagtcttgat cccttaggat cggtgcaatg
    26101 gcgctttgtc atggcggcgg atcatccgct ggcgaacgtt gaagagccgc taacagaagc
    26161 gcagttgcgg cgctttccgg cggtcaatat tgaagacagc gcccgcacct taaccaaacg
    26221 cgtcgcctgg cgattgcctg ggcaaaaaga gattattgtt cctgatatgg aaacgaaaat
    26281 cgccgcccat cttgcgggcg ttggcattgg ttttttgcca aaatcgcttt gccagtcaat
    26341 gatcgataat caacaactag ttagccgggt aatcccaacg atgcgccctc cttcgccatt
    26401 gagtctggca tggcgcaaat ttggcagcgg caaagcggta gaagatattg tgaccttgtt
    26461 tacccagcgc aggccggaaa tcagcggatt tttagaaatt ttcggcaacc cacgcagtta
    26521 aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc gcaagctgat ttgaggttca
    26581 cttttaacta tgcaagagag acacacggaa caggactatc gtgccctgct gattgctgat
    26641 acgcccatta ttgatgttcg cgcccctatc gagtttgagc aaggcgcaat gcccgccgct
    26701 atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg ttggcacctg ctataaacag
    26761 caaggctcag acgcagcgct ggcgctgggg cataaactgg tggcgggtga aattcgtcag
    26821 cagcgcatgg acgcctggcg ggcagcgtgc ctgcaaaatc cgcaaggtat tctctgctgc
    26881 gcccgtggcg gtcagcgctc acatattgtg caaagctggt tgtatgcagc ggggattgat
    26941 tatccgctgg tggaaggcgg ttataaggca ctgcgccaga ccgcgattca ggcgactatt
    27001 gaactggcac aaaaaccgat agtgctgatt ggcggttgta ctggcagcgg taaaacgctg
    27061 ttagtgcagc aacagccgaa cggtgttgat ctggaagggc tggcgcgcca tcgcggttcg
    27121 gcgtttggtc gcacgttaca accacaactt agccaggcga gttttgaaaa cctgctggct
    27181 gccgaaatgc tgaaaaccga cgcccgtcag gatttgcgcc tgtgggtgct ggaagacgaa
    27241 agccggatga tcggttcgaa tcacctgccg gaatgcctgc gcgagcgaat gactcaggcg
    27301 gcgattgcgg tggtagaaga tccgtttgag atccgtcttg agcgcctgaa cgaagagtat
    27361 ttcttgcgta tgcatcatga ttttacccac gcttatggcg acgaacaggg ctggcaggaa
    27421 tattgcgaat acctgcatca cggactttcg gcgattaagc gtcggctggg gttacagcgc
    27481 tataacgaac tcgctgccca gctggatacg gcgctgacaa cgcaactcac caccggcagc
    27541 accgacggtc atctggcctg gctggtgccg ttacttaaag aatattacga cccaatgtat
    27601 cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc gcggtgagtg ggcggaagtg
    27661 gcggaatggg ttaaggcgcg gtaatgaggg acgtatttgt attagtcgcg acttgatctg
    27721 acacatggcc ttgaaaggtt gagagttacc ggttttgata tgagtgtcga atccttatcc
    27781 aaaacaagag gtaactctca tgcttcatac tgccaatccc gtcatcaaac acaaagccgg
    27841 tttgctcaat ctggctgaag aactcagcaa cgtatcaaaa gcctgtaaaa tcatgggcgt
    27901 ttcgcgtgat acgttttatc gttaccgcga actggtcgct gaaggcggtg tggatgcgca
    27961 gattaatcgt agtcgccgcg ctcctaacct taagaaccgt accgatgagg caactgaaca
    28021 ggctgttgtt gattacgccg tcgctttccc gacacatggt cagcaccggg ccagcaacga
    28081 gctgcgtaaa cagggggttt ttatctccga cagtggtgtc cgttctgtct ggttactcca
    28141 taaccttgag aatctcaaaa gacgatactg aacaaatttt atcaggtgac gttccgtaaa
    28201 aagttgtatg gagttctcga tgcagtcggc gcgactatca gtacccccgc tgaaccctgg
    28261 actatcgggc cgccgtactg gggcatgtca ccctggcgtg ccgctggttt tccgctaatg
    28321 gtttatttcc tctttctttc ccggcaagaa tttattttaa tccggaagga ttatttattt
    28381 tcagataggg actcacatta aacacgtaac attttaatta atgcatggct gaatatgtat
    28441 tacacaatgt cagatatcgt aaaagaccag tgcaattcta tgtaaactct ctgactgttc
    28501 attttatttg ttgtttcagg gtcggaactc accctcaata aacaactgaa tacgagtaga
    28561 tattcatcgt ctgagctata tggctttaca caatagccga catgtctttt ctcagtcagg
    28621 aattttactt ttcctcacta cgaagttgac gacttcgata tggaatagac tcttaattca
    28681 agcaattact ctgcacaagc atcaggcaat ccagctccaa tatttgttat aaaataatgc
    28741 gtaacattaa tttcaccaac aaaaaaaagg tttacaccat ccccttgccc tggtatctta
    28801 ccatatttac accacacttt ttttgtatct ggatcattct ttgatacgat tttcgccact
    28861 tgttccctgg ccctcactgt caatgttttc ttaaacctac ccccatcatc atttgttagg
    28921 gaaaaataag aaaaaaaata atcatcttct acatttgtgt atattgatat atccaccgct
    28981 acgtgcggaa aaaaaatctg ggagccatgt aaaaactcca tcattttctg gtattgccat
    29041 atagtttatg taatgtttac ttacaagaga ttcatctttg tataaataaa gataagtaat
    29101 tacgcataaa acaacaatga ttataatagc aaaaat
//
LOCUS       DAZFKM010000057        28918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFKM010000057 DAZFKM010000000
VERSION     DAZFKM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..1018
                     /locus_tag="ACHKAY_002521"
     CDS             164..1018
                     /locus_tag="ACHKAY_002521"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO5701185.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1054..1944
                     /locus_tag="ACHKAY_002522"
     CDS             1054..1944
                     /locus_tag="ACHKAY_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO5701186.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            2005..3504
                     /locus_tag="ACHKAY_002523"
     CDS             2005..3504
                     /locus_tag="ACHKAY_002523"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO5701187.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3505..4494
                     /locus_tag="ACHKAY_002524"
     CDS             3505..4494
                     /locus_tag="ACHKAY_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5701188.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            4516..5478
                     /locus_tag="ACHKAY_002525"
     CDS             4516..5478
                     /locus_tag="ACHKAY_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5701189.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            5475..6521
                     /locus_tag="ACHKAY_002526"
     CDS             5475..6521
                     /locus_tag="ACHKAY_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO5701190.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(6613..7962)
                     /gene="accC"
                     /locus_tag="ACHKAY_002527"
     CDS             complement(6613..7962)
                     /gene="accC"
                     /locus_tag="ACHKAY_002527"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO5701191.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7973..8443)
                     /gene="accB"
                     /locus_tag="ACHKAY_002528"
     CDS             complement(7973..8443)
                     /gene="accB"
                     /locus_tag="ACHKAY_002528"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO5701192.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9309..10139)
                     /locus_tag="ACHKAY_002529"
     CDS             complement(9309..10139)
                     /locus_tag="ACHKAY_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701193.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(10176..11150)
                     /locus_tag="ACHKAY_002530"
     CDS             complement(10176..11150)
                     /locus_tag="ACHKAY_002530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO5701194.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            11302..13242
                     /gene="csrD"
                     /locus_tag="ACHKAY_002531"
     CDS             11302..13242
                     /gene="csrD"
                     /locus_tag="ACHKAY_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO5701195.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13547..14590
                     /gene="mreB"
                     /locus_tag="ACHKAY_002532"
     CDS             13547..14590
                     /gene="mreB"
                     /locus_tag="ACHKAY_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO5701196.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14656..15759
                     /gene="mreC"
                     /locus_tag="ACHKAY_002533"
     CDS             14656..15759
                     /gene="mreC"
                     /locus_tag="ACHKAY_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO5701197.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15759..16247
                     /gene="mreD"
                     /locus_tag="ACHKAY_002534"
     CDS             15759..16247
                     /gene="mreD"
                     /locus_tag="ACHKAY_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO5701198.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            16256..16849
                     /gene="yhdE"
                     /locus_tag="ACHKAY_002535"
     CDS             16256..16849
                     /gene="yhdE"
                     /locus_tag="ACHKAY_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO5701199.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16839..18308
                     /gene="rng"
                     /locus_tag="ACHKAY_002536"
     CDS             16839..18308
                     /gene="rng"
                     /locus_tag="ACHKAY_002536"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO5701200.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18376..22176
                     /gene="yhdP"
                     /locus_tag="ACHKAY_002537"
     CDS             18376..22176
                     /gene="yhdP"
                     /locus_tag="ACHKAY_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO5701201.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22332..23777
                     /gene="tldD"
                     /locus_tag="ACHKAY_002538"
     CDS             22332..23777
                     /gene="tldD"
                     /locus_tag="ACHKAY_002538"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO5701202.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23905..24834)
                     /gene="aaeR"
                     /locus_tag="ACHKAY_002539"
     CDS             complement(23905..24834)
                     /gene="aaeR"
                     /locus_tag="ACHKAY_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO5701203.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            25017..25220
                     /gene="aaeX"
                     /locus_tag="ACHKAY_002540"
     CDS             25017..25220
                     /gene="aaeX"
                     /locus_tag="ACHKAY_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO5701204.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            25228..26160
                     /gene="aaeA"
                     /locus_tag="ACHKAY_002541"
     CDS             25228..26160
                     /gene="aaeA"
                     /locus_tag="ACHKAY_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO5701205.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            26166..28133
                     /gene="aaeB"
                     /locus_tag="ACHKAY_002542"
     CDS             26166..28133
                     /gene="aaeB"
                     /locus_tag="ACHKAY_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO5701206.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            28225..28497
                     /locus_tag="ACHKAY_002543"
     CDS             28225..28497
                     /locus_tag="ACHKAY_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO5701207.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28553..28816)
                     /gene="yhcN"
                     /locus_tag="ACHKAY_002544"
     CDS             complement(28553..28816)
                     /gene="yhcN"
                     /locus_tag="ACHKAY_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO5701208.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6995 a         6887 c         7754 g         7282 t
ORIGIN      
        1 aaagtgaaaa acaaattaag ttattttttt gtaagttatt ttgatgatat tcgacatgca
       61 tcacgctttt tgccgctttt gctgtgctag tttgctttca aaatgaaagt gaatattctc
      121 tttcttcatt ttgtaaggcg aacgacaaat aacaggagct gcgatgtaca tcatttcaag
      181 caaaaacatg ctgaaaaaag cccagtcaga gggatatgcc gtccccgctt tcaacattca
      241 taaccttgaa actttacagg tagttgttga gagcgctgca caacttcgct ctcccgttat
      301 gctggcggga acacccggta cttatcgtta tggtggcgtg ggaagtttaa tttcgttagt
      361 acaatcgctg gctcgcgagt acaacctgcc gttagtcctg catctcgacc atcatgaaga
      421 gagtgatgac atttttaaca aggttcgcgc agggatccgt tctgtcatga ttgatggctc
      481 ccatttgcca tttgaagaca acattgcgcg agtgcaagaa gttaccacct tttgccatcg
      541 ctttgatgtt agcgttgagg ccgagctagg gcgtcttggc ggccaggagg acgatctgct
      601 ggtggatgaa aaagattccg cttatacctc accagccgca gctgcagagt ttgttcaacg
      661 tacccaaatt gactccctgg ccgtagcaat cggcacagca catggtttat atgcgcatga
      721 accacgactg gattttgtgc ggctggaaca gattcgtcag cgagtggaga ttccactggt
      781 gttgcatggt gcttcgggct tatcagaaca ggacgttcag cactgtattc gccgcggtat
      841 ttgtaaagtg aatgtcgcga cagaattgaa aattgcgttt gccgatgcgt taaaaggata
      901 tttctacaac aatcctgcgg caaatgatcc acgccactat atgcagcccg caaaggccgc
      961 catgaaagaa gtcgtaactc gtattattgg tgtctgtggt agtgaaggaa aaatttaaga
     1021 cgttgtaccc tacaaaaaca atgaggattg tttatgaaaa atattaaatt acttggttca
     1081 gctatgttgg tgagcctggc attgttctcg caaaattcgt tggcaaaaga gtataaaatt
     1141 ggtgcgtcat tattaacaca gcaacatcct ttctatattg atcttgcgaa tgccatgaaa
     1201 gaagaggcaa aaaaagacaa tgtcaattta agtgtctcta ttgccaatca ggatttaaac
     1261 aaacaacttt ctgatgttga ggattttatt actaaaaaag tggatgccat tattatctct
     1321 ccggtcgatt cgaaaggcgt ccaggcggcg ataattaaag ccgataaggc cggtattccc
     1381 gttatcaccg tcgacgtcgc tgcggaaggc gttccggtcg tatcgcatgt tgcgaccgat
     1441 aactatgcgg gaggcgttga agcaggcaag ttaatgggca aacttttgaa tggtaaaggt
     1501 actgtagcca ttatttctta tccggcacta caatcggtgg ttgcgcgcgt tgatggtttt
     1561 aagaaagggt taagcgatac accggatatt aaaattgttg ctgaacagcc tggtattacg
     1621 cgggctgaag cgttaacgac tgcgcaaaac attatgcagg caaatgccaa cctgaatggt
     1681 ctgtttggct ttggtgatga tgcggcgctg gcagcggtta tcgcagcaaa atctgcgcat
     1741 aacgacaaca tcaaaatcat tggttttgac ggcatgaaag aagcgcgtga tgccgttgac
     1801 agtgaaaaaa cgtttgcggc cgtgattcgg caatatcccg atcaaatggg tgctaaagcc
     1861 attgatgccg ctgttgatca cctcaatggt aaaccggtag aaaaaatgat cccggtacaa
     1921 cccggcgttt atacaggtaa atagtaaaca tcttcacctc acaacaaaac gttattggcg
     1981 ttttgttgtg acaccgaggt cattatgacg gatgttattc ttgatgtttc gcatattgcg
     2041 aagacatttg gtcatgttca agcattaaaa gatattaccc tctctttacg taaagggcgg
     2101 gtgcatactt tattaggcga gaatggtgcc ggtaaatcga cattaatgaa aatactggcc
     2161 ggtgtttatc cgccaaccca gggaacgata acactacgtg gtgaaactat caccattaat
     2221 aatccgcagc attcacgcca gttaggaatt gcgattattt ttcaggaatt gagcctgagt
     2281 aataatatga ccgtggcgga aaatatttat gccaataatg aaccgcgacg ttttggcatt
     2341 attaacgata aaaaaatgct cgcagattgc cagaatctcc tcgcggaact gggcatacca
     2401 ctagacccac tagaaatggt cggcaatatg tcgatggcac ataggcaact ggtagaaatt
     2461 gctaaagcct taagctatgc agcggatgtc gtgattatgg atgaaccgac atcttcgcta
     2521 agtgataatg aagcggagat tctgtttaac attattgaaa aattaaagca gcgaggctgt
     2581 gcggttattt atatctctca tcgcatggat gaaattatgc ggatttcaga tgatatttcg
     2641 gtgatccgcg acggtgagta tatcgccacg catgaaaaga aaaacagtga tatccagcat
     2701 ttgattgccc aaatggttgg tcgggaaatg aaaaatattt ggccagcacg tcttggggaa
     2761 ataccggatg aaaatgttcc tgcaaaactg gaagtaaaaa atctttccca tccttcgctt
     2821 tttaaagaga tcagtttcgc tgtgcgtccg ggggaggtcc tcggattttt tggcctggtg
     2881 ggggccgggc gttccgatgt gatgaaggcg ctctttggcc tcgtttcgta tcaggggact
     2941 gtgttgattg atggtaagga agtcaggatc gctaatccta aacaggctat tgatcatggc
     3001 attgcttttg tgaccgaaaa ccgcaaggaa gaagggttag tgctgatgca cgacgtcaat
     3061 atgaacacgc accatgtggc gtttcaatat aacgcttcgc ggatgggact catcaatcac
     3121 cgacaagaag aggcgaagac gctgcaatca attgccagaa tgaacactaa agtgagttct
     3181 gtacatcagg cagtgggagc gctgagcggc ggtaatcaac agaaaatcgt gttatcgaag
     3241 tggctggaga aaactccgcg tattttattg ctcgatgagc caacgcgcgg agtggatgtc
     3301 ggcgcaaaat ttgaaatcta taacgtgata cgtcaactgg ctgcggcagg gacagcaatt
     3361 attctggtgt cctctgaact gccagaagtc atggcactta gcgatcgtct ggtggtaatg
     3421 cgtaataaaa cgattgccga tatttattca tgtgaaaatt taacgcagac acaggtaatg
     3481 accgctgcaa caggagtacg ttaaatgaca gagaaaactc ttgccagtcc gaaaccttcg
     3541 gaaattccca ttgcaaataa ttttaaaaaa gtcttcaggc aatatggcgg tatcctcagt
     3601 ggaatggtgg tgttaatcat tctgttcagt tttattaatg acagtttttt taccgccaac
     3661 aacattacta atattattct tcaggtttcg ataattgcaa ttaccgccta tggaatgact
     3721 tatgtacttt tgcttggcga tattgattta tcggtcggtt caaccattgc attgattggc
     3781 acatttgctg cgcttggggc ttcatgggga attccattta ttttattggt cccattatcc
     3841 attattgcag cactggctct ggggatgata aatggtggct tgacggctat agcggggatc
     3901 ccgtcgttta ttgtcactgt tgctactatg gggatcttcc gtggtatcgc ttatatagtg
     3961 accgatggta tgcccatcat gattaaagat gatgcctttc tggcactggg gaatggagaa
     4021 tttctctata tacctattcc aatctggata ttaatcatct tattgctgat taaccacttc
     4081 attcttacta aaacaacctt tggacgaaaa atttatatca ctggtggtaa taaagaagcg
     4141 gcaatttatt cagggataaa tgtcacccgc ttgaaaatta aagtctttat gattacagcc
     4201 gtgctggcag gtatcagcgg gatgatcctc gcctcacgcc gctattccgg gcaaccgaat
     4261 tccgcgttga gctacgaact ggatgctatt gctgcggctg tactgggagg caccagcctg
     4321 aacggcggct atggaaccgt ggtggggacg gtgatcggcg cattaactat cggcgttatt
     4381 aataatggca tgaacttaat gaatgttccc tatttttacc agatggtggt taaaggatta
     4441 gtcattcttg tagccgttta ttttgacgta agaaataaaa gaaaacgcag ctaatcattt
     4501 caaggaatta atcatatgag taaggtcttc acaattggag aaattctggt tgaaattatg
     4561 gcgagtaaaa tcggccagcc atttgatcaa ccaggtatct ggaacggccc ttatcccagt
     4621 ggtgcgcccg ctatttttat cgaccaggtg acacgtcttg gcgttccgtg cggcatcatt
     4681 agctgtgtgg ggaatgatgg ctttggtgat attaatatac atcgtctggc tgctgacggt
     4741 gtggatatca gggggatttc agtcttacct ctggaagcta ccgggagtgc ctttgttact
     4801 tatcataact ctggcgatcg cgattttatt tttaatatta agaatgccgc ttgcggtaaa
     4861 ttatcggcgc agcatgttga tgaaaatatt ctgaaggact gtacccattt tcatattatg
     4921 ggctcttccc tgttttcgtt tcatatggta gacgcagtaa aaaaagcggt aactatcgtt
     4981 aaagcaaatg gtggtgttat ttctttcgac cctaatattc gtaaagagat gctcgatatt
     5041 ccagaaatgc gtgacgccct ccactttgtt cttgagttaa cggatatcta tatgcccagc
     5101 gagggcgaag tgttgctctt gtcgccacat tcgacgccgg agcgtgcaat cgcaggtttt
     5161 cttgaagaag gtgttaaaga ggtgattgtg aagcgcggca atcaaggtgc cagttactac
     5221 tccgcgaatg agcagtttca tgttgagagc tatccggtgg aagaagttga cccgaccggt
     5281 gcgggagact gctttggtgg cgcgtggatt gcctgccgcc agctagggtt tgacgcccat
     5341 cgagcgctgc aatatgccaa tgcctgcggt gcgctggcgg taaccagacg tggcccaatg
     5401 gagggaacgt ctcgccttgc ggaaatcgaa gcgttcatcc agcgccatga catgtccatt
     5461 cgggaagcag cacaatgaaa aacgaaattt ggtcgtggat tggcgatctc tcgcaggttg
     5521 ccggaattaa acactatgag ttacgtagtg gcagagcaaa gggtacagaa gcgtttgatg
     5581 tccgtactgg tgcgggactg gcatttacgg tggtaaaaga tcgcgcgctg gatattgcat
     5641 gggccagcta caaggacacg gcgctttctt ttatcacccc caacggcatt gtcgcccccg
     5701 ctttttttga gtctcagggc aacggatttt tacgtagctt ttacgcgggg ttactgacta
     5761 cctgcggact tagctacatt ggcacgcctt gtgaggatga aggagaaacg ctggggttac
     5821 acgggcgttt ggctgcgact ccagcagaag aggtggggta tcgcacagaa cggaccgacg
     5881 acggcattga atttgtcatc aatggcaaag tacgggaaac gcgactgttc ggcgaaaatc
     5941 tgacgctgga aaggaccatt cgctgccgtt atggcgaaaa tgtgctgcgc attgaagaca
     6001 aggtgactaa tcatggtttc acccgccagc cgctgcaaat tctttatcac tttaattacg
     6061 gctggccttt attatcgcca caggcagaaa tcttgctgtc ggctaaaagc gtaacgccac
     6121 gaactccgca tgcggcggaa gggttaacca gtcatctgga gatttgtacc ccacagcccg
     6181 gttttgacga gcaggtttac tatctcacgt tgaatagcga cagtcagggg atgagtaaag
     6241 ttgctctggt gaacgccgaa ctggggtggg gaatttacga gaaattcgac accatgcagc
     6301 tacccaactt tatccagtgg aaaaatttag gcgcgggtga gtatgttatg ggactggagg
     6361 tcagtaatag ttttcctgac ggtcgtgata aagaacgagc gcaggggcgt ctgcccttta
     6421 ttgagccagg ggaaacaaag aaatactgct ttgagctggg aattgttgat ggcgatgctg
     6481 aaatgagcgc gttgaaagcg gaaattgcag gctaccgtta gctaacggca ataaaaacgc
     6541 cgggcagcgt taacaggccc ggcgttgatt acgttctttg ctaagcgcga tgaaaacacc
     6601 gcgcgtcaca aattattttt cctgaagacc gagttttttc tccagatagt ggatgttagt
     6661 gccaccatgc tggaagttct cgtcattcat gatgcggatc tgcagatcaa cgttggtttt
     6721 gataccgtcg atgatcagct cctgcagcgc attcttcatg cgggcaatcg ccacgtcacg
     6781 gttttcaccg tagcaaatca gcttaccgat cattgagtca tagtacggcg gtacggtgta
     6841 gcccgcgtag atatgagact cccaacgtac gccaaaaccg ccaggtgcgt ggaaacgggt
     6901 gattttgcct gggcttggca ggaaggtgtt cggatcttcg gcgttgatac gacattccac
     6961 cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac agcggttgac cggcagcgat
     7021 acgcagctgt tctttgatca ggtcaacgcc ggtgatcatt tctgtaaccg ggtgttctac
     7081 ctgaatacgg gtgttcattt cgatgaaata gaactcgccg ttttcgaaca ggaactcgaa
     7141 agtacctgca ccgcgatagc cgatatcaac acacgcttta gcgcaacgtt cgccgatgta
     7201 gcgacgcagt tccggggtaa tgcccggtgc tggtgcttct tcgaccactt tctggtggcg
     7261 gcgctgcatg gagcagtcac gttccgccag atagatagcg ttgccctgac cgtcagccag
     7321 tacctgaatc tcgacgtggc gaggattttc caggtatttc tccatgtaaa ccatatcgtt
     7381 gctgaaagca gctttcgctt ccgcacgggt catggagatg gattgtgcca gttcagcgtc
     7441 gccgcgcact acgcgcatac cgcgaccacc gccgccgccg gaggctttga taatcaccgg
     7501 ataaccaatg cgtttagcaa tggcacggtt tttatccata tcgtcgccca acgggccgtc
     7561 agaacccggt acgcaaggga cgcccgcttt tttcatcgcc gcgattgcgg atactttgtc
     7621 gcccatcagg cgaatggttt ctgctttcgg gccgatgaag ataaagccgg agcgttcaac
     7681 ctgctcggca aagttggcgt tctcggagag gaagccgtaa cccggatgga ttgctactgc
     7741 gccggtgatt tcagcggcgc tgatgattgc cgggatgttc agataacttt ttactgacgg
     7801 agcagggcca atacagaccg tttcatctgc cagtaatacg tgttttagat cgcgatccgc
     7861 gctggagtgc acagcgacag tcttgatgcc cagttcttta caggcacgaa gaatacgcaa
     7921 tgcaatctcg ccgcggttgg caataacaat tttatccagc atgttcgcct cgttactcga
     7981 tgacgaccag cggctcgtca aattctaccg gttgtccact ttcgaccaga attgctttca
     8041 cggtaccgga tttgtccgct tcgatctggt tcatcatttt catggcttca acgatgcaca
     8101 gggtatcgcc cacgttgact ttctgaccca cttcgatgaa cgcttttgcg tccgggcttg
     8161 gggtgcggta gaaagtacca accatcgggg aacgtacgat gtgaccactg atttccgctg
     8221 ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc tgcgttagat tgagctggct
     8281 gctgcattat tggtgcagcg taagcttgtt gcatcacagg gaaacttgcg gcaggagctg
     8341 cacggctaat gcgtactgac tcttcgcctt cagaaatttc cagttcggag atgcctgatt
     8401 cttcaaccag ctcgatcagt tttttaatct tacgaatatc catgagtggg ttccgtactc
     8461 tttgtttagt gtgattgtga caggcgtttc accgccgtct gtaaagcgta tgccccgaca
     8521 gctgtatgca tagcgataaa ttccagcagg ccgggacgcc ggtctatttt gccccagagt
     8581 gtcaatgtta gacttgacgg acattgtgca gcgatctgct gctatcctcc ggcaaaaaac
     8641 aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag cgcaaaaact gcgtcaggga
     8701 ggacgaggtc gcacattata acgatttcgt agcaattggc agctaaatac tggtcttatc
     8761 agggaagata atcaacagct aacatgtaaa taaccttcaa caccgtgtaa tttgcaacaa
     8821 gccgcacaat tcacgaaatt agcgccatca tcgacggaac ttcttataac gtaaggtgaa
     8881 aagctgtaaa gccagccctg cgaagatgag gggctgcaat gagataatct tcacagacca
     8941 caaataatgt agggatgcca ggatcgcgac aagatagacg acattacgca gcggttgctg
     9001 gggattaccc aattttcact gcattgtctg gatagatgtt aatgctaaag cacgcaaaat
     9061 taaccgattg acaatgctca gcattaaata ggttcgagtg attaatttgt agtcaaacaa
     9121 aatcagatta ttccccccat tttgcagaag gtaaactgtt ttgtcaggcg gcagtgcggc
     9181 tatctgatgc tttcagaatt tgccacctga caataaaaaa gccgatttgg tagcaaaatc
     9241 ggcttcggtg agtttgcagt tttcatcaca tactccgaca agctttttca agcatgacgg
     9301 aggttgcgtt aatgcaaggt ttctacccag ggataaaagt gaccgttacg gcggatgaat
     9361 atctgtgttg tcagaggatg gtcatcctca cggaagcatt gttttgttag ctgtcgtaat
     9421 gcactgagat ttgctggctg atcattgtgg gtgaaaaaca gttttcctct ggagggaacg
     9481 gtcactaaca ggtcgccgca ttcaggatcg ggtgattgca cccaaagatc gtcaatgagc
     9541 atcagactgg gctcataagt accccctgcg gagataacgg caacttttcc atcgtttgcg
     9601 cctgtatcaa cgatttcagc cgtaacttcc ggcaatattt gcttcaaatt actgatggct
     9661 tgttcgcggt caatggtcac ttcgtagcgc atcagatagc gcatggtttc gctaccatct
     9721 tcagcaaaca agataattaa ctcatcgtta aagtcttccc aggcaaaatc cagcgaggcg
     9781 ttgaggacct ctggatcaga ctcatactgg ctaagctgtt ggcgaacggt atccagccag
     9841 gctttttctt taatcaccgg aacaatacag cgcccggtcg gcagcatttc tgcaagctgt
     9901 ttcagcatat ctgcagccac ttcttgcagc gggtctggtg cggcttgata acgtttgtag
     9961 gcatcatcaa gggtgatttc caccgtttgg tgacccgtat caatgcgcat caatttgtgg
    10021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    10081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    10141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    10201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    10261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    10321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    10381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggcagcc
    10441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    10501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    10561 gggagaatac gactggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    10621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    10681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    10741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    10801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    10861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    10921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    10981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    11041 gaccagtgaa catcaaccgt gacatcgccc tccggcaggc gactttcgtc aagtgtctgt
    11101 accgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    11161 catgtgcatg atggattgga aaataatttc tgaagactat actcgctaat ggaaaagcaa
    11221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    11281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    11341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    11401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    11461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    11521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    11581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    11641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    11701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    11761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    11821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    11881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    11941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    12001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    12061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgatacttgg
    12121 gggcacagcc aggttgaaga acagttcttc actctgacga atctgctgtc gacatttatg
    12181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgctgtta
    12241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    12301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    12361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    12421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    12481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    12541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    12601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    12661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    12721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    12781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    12841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    12901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    12961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    13021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    13081 tccgggacca gcacccaagt ttacgccacc ggtgtgcgtt cgcgaagcga gtggcagacc
    13141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    13201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatctgtt
    13261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    13321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    13381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    13441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    13501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    13561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    13621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    13681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    13741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    13801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    13861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    13921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    13981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    14041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    14101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    14161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    14221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    14281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    14341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    14401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    14461 tttgttaatg gaagaaaccg gcattccagt cgttgttgct gaagacccgc tgacctgtgt
    14521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    14581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    14641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    14701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    14761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    14821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    14881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    14941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    15001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    15061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    15121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    15181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    15241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    15301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    15361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    15421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    15481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    15541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    15601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    15661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    15721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    15781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    15841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    15901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    15961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    16021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    16081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    16141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    16201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    16261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggcgt
    16321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    16381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    16441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    16501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    16561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    16621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    16681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    16741 gataaatggc agctatcacg ccgtagtcgg cttaccgctg gttgaaacgt atgaattatt
    16801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    16861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    16921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    16981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    17041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    17101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    17161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    17221 gctatctggt gtttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    17281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    17341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    17401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa acgcccgcag acccgttatc
    17461 agctgtacgg cgaactggcg ctggcgcagc gtgttctgcg tgatttcgcc gacgccgaac
    17521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attgctggag tttacctcgg
    17581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    17641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    17701 ccggtggtta tctgattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    17761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    17821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg tgggattatc attattgatt
    17881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    17941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    18001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    18061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    18121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgca tctccggcag
    18181 tagctgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    18241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gatgtcgtaa
    18301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    18361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    18421 gttgtgatcg ctgctctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    18481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    18541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    18601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    18661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    18721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    18781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttt
    18841 ctgacgccat ccggtcagcg cgccgagctg gcgatcccac aactcacctg gctgaacgat
    18901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    18961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    19021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    19081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    19141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    19201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    19261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    19321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    19381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    19441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    19501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    19561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    19621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    19681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    19741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    19801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    19861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggcg ctatttcccg
    19921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    19981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    20041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    20101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    20161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    20221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    20281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    20341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgaactg
    20401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    20461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    20521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    20581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccagc
    20641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    20701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    20761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    20821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    20881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    20941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    21001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    21061 gtcggtggtg cagcgagttt cccacaacac ataacgttac gtacgcctat gttgtcactg
    21121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    21181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    21241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    21301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    21361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    21421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    21481 cggcttactg ccgatggtga atgggttaat aatccggaga atgaacgtac ctcgctgaaa
    21541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    21601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcaa
    21661 ccagatgagg cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    21721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgttga tgccctgatg
    21781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    21841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    21901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    21961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    22021 aaccccattg ttggcgcggc agtatttgca gccagtaaag tgctggggcc gctgtggagc
    22081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    22141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    22201 caatctcata tgataatcgt tgccaaaggc caacgagccg gaacataact gtaggccgga
    22261 tagggcgttc acgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    22321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacggcctga
    22381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    22441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    22501 atggttctta caacatcgat cagggcgttg gtgtgcgtgc aatcagtggt gaaaaaaccg
    22561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    22621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    22681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    22741 tcctgcgtcg cgtcgataag gttgcccgcg aagcggacaa gcgcgtacaa gaagtgactg
    22801 ccagcctcag cggcgtttat gaattaattc tggttgcggc caccgacggc acgctggcgg
    22861 cggatgttcg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    22921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    22981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    23041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    23101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aactgccgcg
    23161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    23221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    23281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    23341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    23401 atctgcccat gccgcgtatg accaacacct atatgctgcc gggtaaatcg accccgcagg
    23461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    23521 atattacatc cggcaaattc gttttctcca cttcagaagc gtatctgatt gaaaacggta
    23581 aagtaacgaa gccggtgaaa ggcgcgacgt tgattggttc cggtatcgaa accatgcagc
    23641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    23701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    23761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    23821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    23881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    23941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    24001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    24061 cggcagcagg atctccagtt caccgcgatt gatctcgttg atcacccaca tcagcggcac
    24121 gtaggcgatc ccggcacccg ccgttagcca gcgcaccagc gtcatcggat cattagtcac
    24181 aaatctccct tgcgggatca ggcgagtcga gatcccttcc ggtgcaatca gttcaaattc
    24241 attgtcgggc cgcacgctgt attcaagcca tgaatggcta cttaaatcgg cgggtttttc
    24301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccattg gcatcgcgcc
    24361 aagacggcgg gaaaacaggc tggaatcctg caacgcgccg acgcggatca ccacatccag
    24421 accgtcggca atcaggtcgg gggctggaat tccggtaacc agattgacgc tcaaacctgg
    24481 gtattctttc agcattttgg ctgtcagccc ggcgagaaca ttttgtgcca tagttgaaga
    24541 acagccaatg cgtagcgtcc cgatgggggt gttattgaag gcatacagtt gctcatgaac
    24601 atcctgcact tcatgaagca tacgacggca gccctggtag taaattctac cggcttcggt
    24661 caggccaatg ctgcgtgtgc tacggtttaa cagctttacc tgcaactcat cttccagttt
    24721 tgacaccgtc tgactgatgg acgaaacgct catctgtagc tgtctggcgg cggcggtaaa
    24781 agagccaaat tcaactactt tggcaaacac cgacatgcgt tttagtcgtt ccattattca
    24841 ctctgactta aaagtgattt agatcacata atatagataa cagcataaca gttacgctaa
    24901 tatattaaat atcaatctac agcaatgttg ctctcgcccg gctttccatg cccttctctg
    24961 cggcgacaga tgctgaaaat aataacgcct gctctctcta tacaaccaag gtcaacatga
    25021 gtctgtttcc cgttatcgtg gtgtttgggc tgtccttccc accgatattt tttgaattgc
    25081 ttttatcact ggcgattttc tggctggtgc gccgggtact tgtgccaaca ggtatctacg
    25141 actttgtctg gcatccggcg ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga
    25201 tatcgcgact gttcgtttga ggttgaagtg aaaacactaa taagaaaatt ctcccgtacg
    25261 gccatcacgg tcgtattagt cattctggcc ttcatcgcaa tttttaatgc ctgggtctat
    25321 tacaccgaat ccccctggac gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg
    25381 ccggacgttt ctggactcat tacccaggtg aatgttcatg ataaccagct ggtgaaaaaa
    25441 ggacagatac tgttcaccat cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa
    25501 gccgatgttg cctattatca ggtactggca caggagaaac gccaggaggc cggacgtcgt
    25561 aaccgtctcg gtgtgcaggc gatgtctcgc gaagagatcg accaggctaa caacgtacta
    25621 caaacggttc tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc aaaactggat
    25681 cttgaacgca cggtgatccg cgcgccagca gatggctggg tgaccaacct caacgtctat
    25741 accggtgagt ttattactcg aggatcaacg gcggttgcgc tggtgaaaca gaactccttc
    25801 tatgtactgg cctatatgga agaaactaag ctggaagggg tgcgtccggg gtatcgtgca
    25861 gagatcacgc cgcttggcag taacaaagtg ctgaaaggga ctgttgatag tgttgccgct
    25921 ggggtcacca acgccagcag cacgcgtgac gacaaaggga tggcgaccat cgactctaac
    25981 cttgaatggg tgcgtttggc gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa
    26041 gagaacatct ggcctgcggg caccactgct acagtggtgg tcactggcaa acaagatcgc
    26101 gacgaaagcc aggattcgtt cttccgtaaa atggcccatc gcctgcgtga gtttggttaa
    26161 tcacgatggg tattttctcc attgctaacc aacatattcg ctttgcggta aaactggcga
    26221 ccgccattgt actggcgctg tttgttggct ttcacttcca gctggaaacg ccacgctggg
    26281 cggtactgac agcggcgatt gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt
    26341 attctggcgc tattcgctat cgtggctttt tgcgcatcat cggcacattt attggctgta
    26401 ttgccggact ggtgatcatc attgcgatga tccgcgcacc attattgatg attctggtgt
    26461 gctgtatctg ggccggtttt tgtacctgga tatcctcgct ggtacgaata gaaaactcgt
    26521 acgcgtgggg gctggccggt tataccgcgc tgatcattgt gatcaccatt cagccggaac
    26581 cattgcttac gccgcagttt gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt
    26641 gtgcgattat ggcggatttg ctcttttctc cgcgatcgat caaacaagaa gtggatcgag
    26701 agctggaaag tttgctggtc gcgcaatatc aattaatgca actctgtatc aagcatggcg
    26761 atggtgaagt tgtcgataaa gcctggggcg acctggtgcg acgcaccacg gcgctacaaa
    26821 gcatgcgcag caacctgaat atggaatctt cccgctgggc gcgggccaat cgacgtttaa
    26881 aagcgatcaa tacgctatcg ctgacgctga ttacccaatc ctgcgaaact tatcttattc
    26941 agaatacgcg cccggaattg atcactgata ctttccgcga attttttgac acgccggtag
    27001 aaaccgcgca ggacgtccac aagcagctca aacgcctgcg gagagttatc gcctggaccg
    27061 gggaacggga aacgcctgtc accatttata gctgggtcgc ggcggcaacg cgttatcagc
    27121 ttctcaagcg cggcgttatc agtaacacaa aaatcaacgc caccgaagaa gagatcctgc
    27181 aaggcgaacc ggaagtcaaa gtagagtcag ccgaacgtca tcatgcgatg gttaacttct
    27241 ggcgaaccac actttcctgc attctgggca cgcttttctg gctgtggacg ggctggactt
    27301 ccggcagtgg tgcaatggtg atgattgcgg tcgtgacgtc actggcaatg cgtttgccga
    27361 atccacgcat ggtggcgatc gactttatct acgggacgct ggccgcgctg ccgttagggc
    27421 tgctctactt tttggtgatt atccctaata cccaacagag catgttgctg ctgtgtatta
    27481 gcctggcagt gctgggattt ttcctcggta tagaagtaca gaaacggcga ctgggctcga
    27541 tgggggcgct ggccagcacc ataaatatta tcgtgctgga taacccgatg actttccatt
    27601 tcagtcagtt tctcgacagc gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg
    27661 ttattttgct tgtgcgggat aaatcgcgcg acaggaccgg acgtgtactg cttaatcagt
    27721 ttgtttctgc cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc
    27781 tcccggcact ttatcagcag ctgtttttgc tgatgaataa gttcccaggg gatttgccga
    27841 aatttcgcct ggcgctgacg atgattatcg cgcaccagcg cctgcgtgat gcgccgatcc
    27901 cggttaacga ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga
    27961 tatctgcccg tagcgatgat aaacgtcgtc ggtactttgg ccagttgctg gaagaactgg
    28021 aaatctacca ggaaaagcta cgcatctggc aagcgccacc gcaggtgacg gaaccggttc
    28081 atcggctggc ggggatgctc cataagtatc aacatgcgtt gaccgatagt taagtcaaaa
    28141 ccgacgccaa aagcgtcggt tttttcatgg ctatacttag cgatgcacgg cagaactcgc
    28201 cgcgaaacgt gacggtggca acagatgaat atttatacct ttgattttga tgagattgag
    28261 agtcaggagg atttttatcg tgactttagc caaacctttg gtctggctaa agataaggta
    28321 cgcgatctcg actcactatg ggatgtgtta atgaacgatg tcctgccgct accacttgag
    28381 attgaatttg ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg
    28441 tttgatgagg cagaggaaga gctggaaggg catttgcgtt ttaatgttcg tcattagcgc
    28501 aaaaaaaagc ccccgaaccg ggggcaatat cgtcggacaa gacgatgagg gtttatttgt
    28561 acagttcagc cgtagcgtgc caggtgtcac cgctacgagc ttcagtaatc tggtaggccg
    28621 ttgcgccttt ctcttccgct tttttgttca gcatttcacg catatccatt ggcgaagacg
    28681 ccacaccact tacggatacg gtcccgattg cttcacgatt ttgtgcttgt gcagcatcaa
    28741 tggagtcggc agcgaatgca ccgaaagaga gaacagaaag tacgcttaat gcagctacag
    28801 tggttttgat tttcatgatt tttacctcga cataatcttt tagctgggcc tttgtttcgt
    28861 gacccatatc acaaaatcaa gtatacacta attattgaac taattaatac cacgctaa
//
LOCUS       DAZFKM010000058        28466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFKM010000058 DAZFKM010000000
VERSION     DAZFKM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            494..1633
                     /gene="wza"
                     /locus_tag="ACHKAY_002545"
     CDS             494..1633
                     /gene="wza"
                     /locus_tag="ACHKAY_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO5701209.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1639..2082
                     /gene="wzb"
                     /locus_tag="ACHKAY_002546"
     CDS             1639..2082
                     /gene="wzb"
                     /locus_tag="ACHKAY_002546"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO5701210.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2085..4247
                     /gene="wzc"
                     /locus_tag="ACHKAY_002547"
     CDS             2085..4247
                     /gene="wzc"
                     /locus_tag="ACHKAY_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO5701211.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4340..5179
                     /gene="wcaA"
                     /locus_tag="ACHKAY_002548"
     CDS             4340..5179
                     /gene="wcaA"
                     /locus_tag="ACHKAY_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO5701212.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5182..5670
                     /gene="wcaB"
                     /locus_tag="ACHKAY_002549"
     CDS             5182..5670
                     /gene="wcaB"
                     /locus_tag="ACHKAY_002549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO5701213.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5667..6884
                     /gene="wcaC"
                     /locus_tag="ACHKAY_002550"
     CDS             5667..6884
                     /gene="wcaC"
                     /locus_tag="ACHKAY_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO5701214.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6859..8076
                     /gene="wcaD"
                     /locus_tag="ACHKAY_002551"
     CDS             6859..8076
                     /gene="wcaD"
                     /locus_tag="ACHKAY_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO5701215.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8087..8833
                     /gene="wcaE"
                     /locus_tag="ACHKAY_002552"
     CDS             8087..8833
                     /gene="wcaE"
                     /locus_tag="ACHKAY_002552"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO5701216.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8849..9397
                     /gene="wcaF"
                     /locus_tag="ACHKAY_002553"
     CDS             8849..9397
                     /gene="wcaF"
                     /locus_tag="ACHKAY_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO5701217.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9423..10544
                     /gene="gmd"
                     /locus_tag="ACHKAY_002554"
     CDS             9423..10544
                     /gene="gmd"
                     /locus_tag="ACHKAY_002554"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO5701218.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10547..11512
                     /gene="fcl"
                     /locus_tag="ACHKAY_002555"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10547..11512
                     /gene="fcl"
                     /locus_tag="ACHKAY_002555"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO5701219.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11515..11994
                     /gene="gmm"
                     /locus_tag="ACHKAY_002556"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11515..11994
                     /gene="gmm"
                     /locus_tag="ACHKAY_002556"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO5701220.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11991..13214
                     /gene="wcaI"
                     /locus_tag="ACHKAY_002557"
     CDS             11991..13214
                     /gene="wcaI"
                     /locus_tag="ACHKAY_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO5701221.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13217..14653
                     /gene="cpsB"
                     /locus_tag="ACHKAY_002558"
     CDS             13217..14653
                     /gene="cpsB"
                     /locus_tag="ACHKAY_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO5701222.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14846..16216
                     /gene="cpsG"
                     /locus_tag="ACHKAY_002559"
                     /gene_synonym="manB"
     CDS             14846..16216
                     /gene="cpsG"
                     /locus_tag="ACHKAY_002559"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO5701223.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16352..17746
                     /gene="wcaJ"
                     /locus_tag="ACHKAY_002560"
     CDS             16352..17746
                     /gene="wcaJ"
                     /locus_tag="ACHKAY_002560"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO5701224.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17748..19226
                     /gene="wzxC"
                     /locus_tag="ACHKAY_002561"
     CDS             17748..19226
                     /gene="wzxC"
                     /locus_tag="ACHKAY_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO5701225.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19372..20652
                     /gene="wcaK"
                     /locus_tag="ACHKAY_002562"
     CDS             19372..20652
                     /gene="wcaK"
                     /locus_tag="ACHKAY_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO5701226.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            20649..21869
                     /gene="wcaL"
                     /locus_tag="ACHKAY_002563"
     CDS             20649..21869
                     /gene="wcaL"
                     /locus_tag="ACHKAY_002563"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO5701227.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21880..23274
                     /gene="wcaM"
                     /locus_tag="ACHKAY_002564"
     CDS             21880..23274
                     /gene="wcaM"
                     /locus_tag="ACHKAY_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO5701228.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23449..24342
                     /gene="galF"
                     /locus_tag="ACHKAY_002565"
     CDS             23449..24342
                     /gene="galF"
                     /locus_tag="ACHKAY_002565"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO5701229.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            24714..25799
                     /gene="rfbB"
                     /locus_tag="ACHKAY_002566"
     CDS             24714..25799
                     /gene="rfbB"
                     /locus_tag="ACHKAY_002566"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO5701230.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            25799..26698
                     /gene="rfbD"
                     /locus_tag="ACHKAY_002567"
     CDS             25799..26698
                     /gene="rfbD"
                     /locus_tag="ACHKAY_002567"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO5701231.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26756..27634
                     /gene="rfbA"
                     /locus_tag="ACHKAY_002568"
     CDS             26756..27634
                     /gene="rfbA"
                     /locus_tag="ACHKAY_002568"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO5701232.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27639..28181
                     /gene="rfbC"
                     /locus_tag="ACHKAY_002569"
     CDS             27639..28181
                     /gene="rfbC"
                     /locus_tag="ACHKAY_002569"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO5701233.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
     gene            28181..>28466
                     /locus_tag="ACHKAY_002570"
     CDS             28181..>28466
                     /locus_tag="ACHKAY_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701234.1"
                     /translation="MLKKKLQKIKEYHSVLELAIIQGANAIFPVLVFPFFLITLGENI
                     FSSIAVGEVLALYVLIFSLYSFDIISVQKVISSVTKDEIFKVYILTLICRL"
BASE COUNT         7161 a         6853 c         7597 g         6855 t
ORIGIN      
        1 aaatatcgca tgtggaggcg caatattaaa tattgtcaac ctaaagaaac tcctaaaaac
       61 catattgaat gacacttaat ataattctta aaaatagcca attaccgaat tgttaccttg
      121 cctgctattc cgttagctgt aacacttcct cctgcattat tggagagcca atattcaatt
      181 accataatgt caccttatat tcggaagtta atttccgcaa taaccctttc tcatcaacga
      241 ttgtatgcag agagggtttg aaaaattaag ctaaatcagt gtattggtag ctaaaaagcc
      301 aggggcggta gcgtgtctgg atgcctgaaa gaccagtctg aattattctg tcaatagcct
      361 gcggatgaaa tcaatttttt taggactgat gccagttaaa tttttattca attaactgca
      421 aggtaaatgt gcatggcgac attgctatta atagtgcaca ggataattac tctgccaaag
      481 tgataaataa acaatgatga aatccaaaat gaaattgatg ccattattgg tgtcagtaac
      541 cttgataagc ggttgcacag tacttccggg cagcaatatg tcgacgatgg gcaaagacgt
      601 catcaaacag caggacgctg atttcgatct cgacaaaatg gtgaatgttt atccgctgac
      661 cccgcgcctg attgaccaat tacgcccacg cccgaatgta gcgcgcccca atatgacgct
      721 ggaaagcgag atcgcgaatt atcagtatcg cgtcgggccg ggggacgttc ttaatgtcac
      781 cgtctgggat cacccggaac tcaccacgcc agccggtcag taccgcagct ccagcgacac
      841 cggcaactgg gtacagcctg acggcactat gttttacccg tatatcggca aggttcacgt
      901 agtcgggaaa acgctcgctg aaatccgcag tgatattacc gggcgcttag cgacgtacat
      961 cgctgacccg caggtggacg ttaatatcgc cgccttccgc tcgcaaaagg cctatatctc
     1021 aggtcaggtg aataaatccg gtcaacaggc gatcaccaac gtaccactga ccattctcga
     1081 cgccatcaac gccgcaggtg gcctgaccga caccgctgac tggcgcaacg tggtgctaac
     1141 acacaatggt cgtgaagaac gcatttcttt gcaggcgctg atgcaaaacg gcgacctcaa
     1201 tcagaaccga ctgctttatc ccggcgatat tctctacgtg ccgcgtaatg atgatctgaa
     1261 agtatttgtg atgggtgaag tgaagaaaca gagcaccctg aaaatggact ttagcggcat
     1321 gaccctgact gaagctctgg gcaatgccga aggcatcgac atgaccacct ccaacgccag
     1381 cggcatcttt gtcattcgtc cgttgaaagg cgagggcggg cgtaacggca agattgccaa
     1441 tatctaccag ctggatatgt ccgatgcgac gtcgctggtg atggcgacag aattccgcct
     1501 gcaaccttat gacgtggtgt atgtcaccac cgccccggtt tcccgctgga accgtctgat
     1561 caatcagttg ctgccaacta ttagcggtgt ccgttacatg acggatacag ccagcgacat
     1621 tcataactgg taatcgtcat gtttaacaac atcttagttg tctgtgtcgg caatatttgc
     1681 cgttccccga cggcggaacg cttactgcaa cgttatcacc cggagctgaa agtggagtcc
     1741 gctggactcg gcgcgctggt cggtaagggc gctgatccta ccgctatcag cgtcgccgca
     1801 gaacatcaac tgtctctgga aggtcactgt gcacggcaaa tcagccgccg cctgtgtcgc
     1861 aactatgacc tgattttgac catggaaaag cgccatatcg aacgcttatg cgagatggca
     1921 cctgagatgc gcggcaaagt gatgctgttt ggtcactggg ataacgaatg tgaaatcccc
     1981 gatccgtatc gcaaaagccg ggaaacgttt gcagcggtgt acacattact tgaacggtct
     2041 gcccgccagt gggcgcaggc attgaacgca gagcaggtat aagaatgaca gaaaaagtaa
     2101 aacaacatgc cgctccggta acgggcagtg atgaaatcga tattggtcgc ctggtcggca
     2161 ccgtcattga agcgcgctgg tgggtgattg gcatcaccgc tgtatttgcc ctctgtgctg
     2221 tggtttacac tttcttcgcc acgccgattt atagtgccga cgcactggta caaatcgaac
     2281 aaaacagcgg caattcgtta gtgcaggaca ttggctcggc attagccaac aaaccgcctg
     2341 catcggacgc cgagatccag ttgattcgtt cgcgtctggt gcttggtaaa acggtggacg
     2401 atctagacct cgatattgcg gtgagcaaaa acacgttccc gattttcggt gcgggctggg
     2461 atcgtttgat gggacgtcag aacgagacgg tgaaagtgac cacctttaac cgcccgaaag
     2521 agatggcgga ccaggtgttt acgcttaatg tgctggacaa taaaaactac accctgagca
     2581 gtgatggcgg ctttagcgcc cgtgggcaag cgggccagat gctgaaaaaa gaaggcgtca
     2641 cgctgatggt tgaagccatt cacgcccgcc cgggcagtga gtttaccgtc accaaatact
     2701 ccacgctggg gatgatcaat caactgcaaa acagcctgac ggtaacggag aacggcaaag
     2761 acgcaggcgt actgagcctg acttataccg gtgaagatcg tgaacagatc cgcgacattc
     2821 ttaacagcat cgcccgtaac tatcaggaac aaaatattga gcgcaaatcg gcggaagcgt
     2881 cgaaaagcct cgctttcctc gcgcaacagt taccggaagt acgtagccgc ctcgatgttg
     2941 ccgaaaacaa actgaatgcc ttccgtcagg ataaagattc tgttgatctg ccgctggaag
     3001 cgaaagcggt gctcgattcg atggtgaaca tcgacgcgca gttgaacgaa ctgaccttta
     3061 aagaggcgga aatctccaag ctgtacacca aagttcaccc cgcgtaccgc acgctgctgg
     3121 agaaacgtca ggcgctggaa gacgaaaaag ccaaacttaa tggtcgcgta acggcgatgc
     3181 cgaaaaccca gcaggaaatt gtccgtctga cccgcgatgt cgaatctggt cagcaggtct
     3241 atatgcaact gctgaataaa gagcaggagc tgaaaatcac cgaagccagc accgtcggcg
     3301 atgtgcgcat tgttgacccg gcaatcactc agcctggtgt gctaaaaccg aagaaagggc
     3361 tgattatcct cggcgcgatt atccttggcc tgatgctctc tatcgtgggt gtgctgctgc
     3421 gctcgttgtt taatcgcggc atcgaaagcc cgcaggtgct ggaagaacac ggtatcagcg
     3481 tctatgccag catcccgttg tcggaatggc agaaagcgcg cgatagcgtc aaaaccatca
     3541 aagggattaa acgctataaa cagagccagc tactggcggt ggggaatcca accgatctgg
     3601 cgattgaagc catccgcagt cttcgtacca gtttgcactt cgcgatgatg caggcgcaga
     3661 acaatgtgtt gatgatgacc ggggttagcc cgtcaatcgg taaaaccttt gtctgcgcca
     3721 acctggcggc ggtgatcagc cagaccaata aacgcgtgtt gttgatcgac tgcgatatgc
     3781 gcaaaggcta cacccacgag ctgttgggca ctaataacgt taatggcctg tcggaaattc
     3841 tgattggtca gggcgatatt actaccgctg ctaaaccgac ctctattgcc aaatttgacc
     3901 tgatcccgcg cggtcaggtg ccgccaaatc cttctgaact gttgatgagc gaacgctttg
     3961 ccgaactggt gaactgggcg agtaaaaact atgacctggt gttgattgat acgccgccga
     4021 ttctggcagt gaccgatgcg gcaattgttg gtcgtcatgt tggaaccacg ttaatggtgg
     4081 cgcgttatgc ggtcaacaca ttgaaagaag tggaaaccag tctgagccgc tttgagcaaa
     4141 atggtattcc ggtgaaaggg gtgattctga actccatctt ccgccgcgcc agcgcgtatc
     4201 aggattatgg ctattacgaa tacgaatata agtcggatgc gaaataacga cgcctgcatt
     4261 cgcaccgccc cgtaggccgg ataaggcgct cacgccgcat ccggcaagca aaccagctca
     4321 taagccggga gaacaaccta tgaaagacaa tccgctgatc tcaatctata tgccaacctg
     4381 gaaccgccaa caactggcga ttcgggcaat aaaatcggtt ttgcgccagg actacagcaa
     4441 ctgggagatg atcatcgtgg atgattgctc cacctcctgg gaacaactgc aacagtacgt
     4501 caccgccctc aacgatccgc gtatcactta cattcacaac gatattaaca gcggggcgtg
     4561 cgcggtacgt aaccaggcga ttatgctggc gcaaggggaa tatatcaccg ggattgatga
     4621 cgatgacgaa tggacaccca accgtctgag cgtcttcctc gcccataaac agcaactggt
     4681 cacacacgcc tttttgtacg ctaacgacta cgtatgccag ggagaagttt attcccaacc
     4741 ggcaagctta ccactgtatc ccaaatcgcc gtactcacgc cgcctgttct ataaacgcaa
     4801 tatcattggc aatcaggtct ttacctgggc atggcgtttc aaagagtgtt tgttcgatac
     4861 cgaactgaaa gcagcacagg attacgacat cttcctgcgg atggtggtgg agtacggcga
     4921 accgtggaaa gtggaagagg cgacgcagat cctgcatatc aatcacggtg aaatgcagat
     4981 cacctcgtca ccgaaaaaat tctccgggta cttccacttt taccgcaagc acaaagataa
     5041 atttgaccgt gcaagcaaaa aatatcagct gtttaccctc taccagatcc gcaataaacg
     5101 tatgacctgg cgcactttgc tgacgctgct gtcggtgcgt aacggcaaac gtctggctga
     5161 tggtattcgg gggcgctaaa gatgctggaa gatctgcgtg ccaacagctg gagtttacgc
     5221 ccgtgctgca tggttcttgc ctatcgtgtt gctcattttt gttcggtgtg gcgcaaaaag
     5281 aacgtcctca acaatctgtg ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa
     5341 tgctttttcg gttatgaaat ccaggccgcc gcgaccattg gccgccgctt tactatccat
     5401 cacggttacg ccgtagtgat caataaaaac gtggtagcgg gggatgattt caccattcgc
     5461 cacggcgtca ctatcggcaa tcgtggtgcc gataacatgg catgtccaca cattggcaac
     5521 ggcgtcgaac tcggtgccaa cgtcattatt cttggtgata tcacgcttgg taacaacgtc
     5581 accgtgggcg cgggcagcgt ggtgctcgac tctgtcccgg acaacgcgct ggtggtggga
     5641 gaaaaagcgc gagtgaaggt aattaaatga atattctgca atttaatgtg cgactggcgg
     5701 aaggcggggc ggcaggtgtg gcgttagatc tccatcagcg tgcgctgcaa caggggctgg
     5761 cgtcacattt tgtctacggc tacggcaaag gcggcaaaga gagcgtcagc catcaaaact
     5821 atccgcaggt cgtcaaacat acgccgcgga tgaccgcgat ggcgaacatt gcgttgtttc
     5881 gtctgtttaa tcgcgatctg tttggcaatt tcaatgagtt atatcgcacc attacttgca
     5941 caccgggtcc ggtggtcctg cattttcatg tgctgcacag ctactggcta aatcttaaga
     6001 gcgtggtgcg cttttgcgaa aaggtgaaaa accacaaacc ggatgtcact ctggtctgga
     6061 cgctgcacga ccactggagc gttaccgggc gctgcgcctt tactgacggt tgtgaaggct
     6121 ggaaaacggg ctgccagaaa tgcccgacct taaataatta tccgccggtg aagattgatc
     6181 gggcgcacca gctggtggcg ggcaaacgcc agttattccg tgagatgctg gcgctgggct
     6241 gtcagtttat ttcccccagc cagcatgtgg ctgacgcttt caatagcctg tacggtccag
     6301 ggcgttgccg gattatcaat aacggtattg atatggcaac cgaagcgatt ctggcggatc
     6361 tgcctccggt gcgcgaaacc cagggtaagc cgaaaatcgc ggtggtggcg catgacctgc
     6421 gttacgacgg caaaactaac cagcaactgg tgcgtgagat gatggcgctg ggcgacaaaa
     6481 tcgaactgca tacctttggt aagttctcgc cgttcaccgc tggcaacgtg gttaatcacg
     6541 gctttgaaac tgacaagcgc aagttgatga gcgcgctcaa tcagatggat gcgctggtgt
     6601 tcagttctcg cgtcgataac tacccgctga ttttgtgtga ggcgctatcg attggcgtgc
     6661 cggtgattgc cacccatagc gacgcggcgc gggaagtgct gcaaaaatcc ggcggtaaaa
     6721 ccgtcagcga agaagacgtg ctgcaactgg tgcagttaag caaaccggaa atcgcgcagg
     6781 cgatatttgg taccacgctg gctgagttca gccaacgcag ccgcgcggcc tacagtggac
     6841 aacagatgct ggaggagtat gtcaacttct atcagaatct gtagctacct gttgttgccg
     6901 ctgatttatc tgctggttaa cgtcaaaatc gcccagcttg gcgaaagttt ccccatcacc
     6961 atcgtcactt ttttacctgt cttgttactg ctgtttttag agcgcatcag cgttaaaaaa
     7021 ttgatgattg ccttagggat tggtgcggga ctcacggcgt ttaactatct gtttggtcag
     7081 tcgctggatg ccagtaaata cgtcacttca actatgctgt ttgtctatat tgtgatcatt
     7141 attggcatgg tgtggagtat tcgttttaaa acaatttcgc cacacaacca tcgcaagata
     7201 ttacgtttct tttatctggt ggtcgggttg gtggtggcgc tggcggcggt ggagatggcg
     7261 caaattatcc tcaccggcgg cagcagtatt atggagtcga tttcgaaata tctgatttac
     7321 agcaacagct atgtactgaa tttcattaaa ttcggcggca agcgcacgac agcactttat
     7381 ttcgaaccgg catttttcgc tctggcatta atctcaattt ggctcagcat caaacagttt
     7441 ggtatcaaaa cgcctaaaac cgatgctatg attctcgcag ggataatatt atccggatcg
     7501 ttttcagggg ttatgacctt tatcctgttt tatttgctgg agtgggcatt tcaatatctg
     7561 aataaagagg cgattaagaa aaagttaccg ttagcattga tttctctggc tgtattcctg
     7621 gttggtgtgg taatcgcgtt tccttatatt tccacccgtc tgggcgattt aggtacggaa
     7681 ggatcgtcat cgtattatcg tattgtcggt ccgctggtga tggtcggtta ttctttgacc
     7741 catattgacg gtgtagtcag atttggctca ctttatgaat atgtcgcatc attcggaata
     7801 tttaacggtg cggatgtcgg aaaaaccata gacaatggtt tgtatctgct gattatttat
     7861 ttttcctggt tcgcagtgtt tttatcgctg tggtacatgg ggaaagtgat aaaaatgatg
     7921 atcaacgctt ttggtgataa ccgcaatttt cgcgtgcaat tatatctttt tactccggtg
     7981 tcgctgtttt ttaccggttc gatatttagc ccggaatatg catttttaat cgtctgtccg
     8041 tttattttgc gaaaagcgtt aaatattacg aggtaagaat aagaacatgt tgcttagcat
     8101 aatcactgtc gcgtttcgta acctcgaagg gatagtcaaa acacatgcct cgctggcgca
     8161 tctggcgcag gcggaagata tcagcttcga atggattgtt gtcgatggcg gttccaatga
     8221 cggcacccgt gagtatctgg aaaatctcaa tggtatctat aacctacgct ttgtcagcga
     8281 gccagataac ggtatctacg acgccatgaa caaaggcatt acgatggcac aaggcaagtt
     8341 cgcgttgttt ctcaattcgg gcgatatttt tcatcaggat gccgcaaatt ttgtccgtaa
     8401 gttaaaaatg caaaaagata acatgatgat caccggcgat gcgctgctgg attttggcga
     8461 cgggcataaa attaaacgta gcgccaaacc gggctggtat atttatcaca gcctgcccgc
     8521 cagtcatcag gcgatatttt tccctgtatc cggcttgaaa aaatggcgtt atgacctgga
     8581 atataaagtt tcttccgact acgcgctggc agccaaaatg tataaagccg gttatgcatt
     8641 taaaaaactc aatggcctgg tgtctgaatt ttccatgggt ggggtatcta ccaccaataa
     8701 tatggaattg tgtgctgacg cgaaaaaagt ccaacgacaa atattacatg tacctggctt
     8761 ttgggctgaa ttatcctggc atttacgcca acgtactacc tcaaagacga aagccttata
     8821 taacaaagtc tgaatataag gagaaccaat gcaagattta agcggtttct cggtgccgaa
     8881 agggttccgg ggcggcaacg ctattaaagt gcaattatgg tgggcagtac aggcaacaat
     8941 atttgcctgg tcgccacaag tattgtatcg ctggcgggct tttttattac gtttattcgg
     9001 agcaaaaata ggaaaaaacg tagttattcg tccgtcagta aaaattacct atccgtggaa
     9061 attaacctta ggtgattacg cgtgggtcgg cgatgacgtc aatttatata ccctcggtga
     9121 aataaccatt ggcgcacatt cggtgatatc gcaaaaaagt tatttatgca ccggtagcca
     9181 cgaccatgca agtcaacatt tcaccattaa cgccacgcct attgtgattg gcgagaaatg
     9241 ctggctggca accgatgtct ttgttgcccc aggcgtcaca atcggcaacg gcaccgtcgt
     9301 gggtgcacga agcagtgttt ttaaatcgct tccggcaaat gtggtttgcc gggggaatcc
     9361 cgcagtggtg atacgcgaac gcgttgaaac tgaataaatt caaaaataca gaggaataag
     9421 acatgtcaaa agtcgctctc atcaccggtg taaccggaca agacggttct tacctggcag
     9481 agtttctgct ggaaaaaggt tacgaggtgc atggtattaa gcgtcgcgca tcgtcattca
     9541 acaccgagcg cgtggatcac atttatcagg atccgcacac ctgcaacccg aaattccatc
     9601 tgcattatgg cgacctgagt gatacctcta acctgacgcg cattttgcgt gaagtacagc
     9661 cggatgaagt gtacaacctg ggcgcaatga gccacgttgc ggtctctttt gagtcaccag
     9721 aatataccgc tgacgtcgac gcgatgggta cgctgcgcct gctggaggcg atccgcttcc
     9781 tcggtctgga aaagaaaact cgtttctatc aggcttccac ctctgaactg tacggtctgg
     9841 tgcaggaaat tccgcagaaa gaaaccacgc cgttctaccc gcgatctccg tatgcggtcg
     9901 ccaaactgta cgcctactgg atcaccgtta actaccgcga atcctacggc atgtacgcct
     9961 gtaacggtat tctcttcaac catgaatccc cgcgccgcgg cgaaaccttc gttacccgca
    10021 aaatcacccg cgcaatcgcc aacatcgctc aggggctgga gtcgtgcctg tacctcggca
    10081 atatggattc cctgcgtgac tggggccacg ccaaagacta cgtaaaaatg cagtggatga
    10141 tgctgcaaca ggaacagcca gaagatttcg ttatcgcgac cggcgttcag tactccgtac
    10201 gtcagttcgt ggaaatggcg gcagcacagc tgggcatcaa actgcgcttt gaaggcacgg
    10261 gcgttgaaga gaagggcatt gtggtttccg tcaccgggca tgacgcgccg ggcgttaaac
    10321 cgggcgatgt gattatcgcc gttgacccgc gttacttccg tccggcggaa gttgaaacgc
    10381 tgctcggcga cccgaccaaa gcgcacgaaa aactgggctg gaaaccggaa atcaccctca
    10441 gagagatggt gtctgaaatg gtggctaatg acctcgaagc ggcgaaaaaa cactctctgc
    10501 tgaaatctca cggctacgac gtggcgatcg cgctggagtc ataagcatga gtaaacaacg
    10561 aatttttatc gctggccatc gcgggatggt cggttccgcc atcaagcgac agctcgaaca
    10621 gcgcggtgat gtggaactgg tattacgcac ccgcgacgag ctgaacctgc tggacagccg
    10681 cgccgtgcat gatttctttg ccagcgaacg tattgaccag gtctatctgg cggcggcgaa
    10741 agtgggcggc attgttgcca acaacaccta tccggcggat ttcatctacc agaacatgat
    10801 gattgagagc aacatcattc acgccgcgca tcagaacgac gtgaacaaac tgctgtttct
    10861 cggatcgtcc tgcatctacc cgaaactggc aaaacagccg atggcagaaa gcgagttgtt
    10921 gcagggcacg ctggagccga ctaacgagcc ttatgctatt gccaaaatcg ccgggatcaa
    10981 attgtgcgaa tcttacaatc gccagtacgg acgagattac cgttcagtca tgccgaccaa
    11041 cctgtacggg ccacacgaca acttccaccc gagtaattcg catgtgatcc ctgcattgct
    11101 gcgtcgcttc cacgaggcga cggcacagaa tgcgccggac gtggtggtgt ggggcagcgg
    11161 tacaccgatg cgtgaattcc tgcacgtcga tgatatggcg gcggcaagca ttcatgtgat
    11221 ggagctggcg cacgaagtct ggctggagaa cacccagccg atgctgtcgc acattaacgt
    11281 cggcacgggc gttgactgca ctatccgcga gctggcgcaa accatcgcca aagtggtggg
    11341 ttacaaaggt cgggtggttt ttgatgccag caaaccggat ggcacaccgc gcaaactgct
    11401 ggatgtgatg cgcctgcatc agcttggctg gtatcacgaa atatcactgg aagcggggct
    11461 tgccagcact taccagtggt tccttgagaa tcaagaccgc tttcgggggt aatgatgttt
    11521 ttacgtcagg aagactttgc cacggtagtg cgctccacac cgcttgtctc tctcgacttt
    11581 attgtcgaga acagtcgcgg cgagtttctg cttggcaaaa gaaccaaccg cccggcgcag
    11641 ggttactggt ttgtgccggg agggcgcgtg cagaaagacg aaacgctgga agccgcattt
    11701 gagcggctga cgatggcgga actggggctg cgtctgccga taacagcagg ccagttttac
    11761 ggtgtctggc agcactttta tgacgataac ttctccggca cggatttcac cactcactat
    11821 gtggtgctcg gttttcgctt cagagtcgct gaagaagatc tgttactgcc ggatgagcag
    11881 catgacgatt accgctggct gacgccggac gcgctgctcg ccagtgacaa tgttcacgct
    11941 aacagccgcg cctattttct cgctgagaag cgtgccggag tacccggatt atgaaaatac
    12001 tggtctacgg cattaactac tcgccggagt taaccggcat cggcaaatac accggcgaga
    12061 tggtggaatg gctggcggca caaggtcatg aggtgcgggt cattaccgca ccgccttact
    12121 acccgcagtg gcaggtgggc gagaactatt ccgcctggcg ctacaaacga gaagaggggg
    12181 ccgccacggt gtggcgctgc ccgctgtatg tgccaaaaca gccgagcacc ctgaaacgcc
    12241 tgttgcatct cggcagtttt gccgtcagca gttttttccc gctgatggcg caacgtcgct
    12301 ggaagccgga gcgcattatc ggcgtagtgc caacgctgtt ttgcacgccg ggaatgcgtc
    12361 tgctggcgaa actctctggc gcgcgtaccg tgctgcatat tcaggattac gaagtagatg
    12421 ccatgctggg gctgggcctt gccggaaaag gcaaaggcgg caaagtggca cagctggcaa
    12481 cggcgttcga acgtagcgga ctacataacg tcgataacgt ctccacgatt tcgcgttcga
    12541 tgatgaataa agccatcgaa aaaggcgtgg cggcggaaaa cgtcatcttc ttccccaact
    12601 ggtcggaaat cgcccgtttt cagcatgttg cagatgccga tgttgatgcc cttcgtaacc
    12661 agcttggcct gccggataac aaaaaaatca ttctttactc cggcaatatt ggtgaaaagc
    12721 aggggctgga aaacgttatt gaagctgccg atcgcctgcg cgatgaacca ctgatttttg
    12781 ccattgtcgg gcagggtggc ggcaaagcgc gactggaaaa aatggcgcag cagcgtggac
    12841 tgcacaacat gcaatttttc ccgctgcaat cgtatgacgc tttacccgca ctgctgaaga
    12901 tgggcgattg ccatctggtg gtgcaaaaac gcggcgcggc agatgccgta ttgccgtcga
    12961 aactgaccaa tattctggcg gtaggcggta acgcggtgat taccgcagag gcccacacag
    13021 aactggggga gctttgcgaa accttttcgg gcattgcggt ttgcgtagaa ccggaatcgg
    13081 tcgaggcgct ggtggcgggg atccgtcagg cgctcctgct gcccaaacac aacacggtgg
    13141 cacgtgaata tgccgaacgc acgctcgata aagagaacgt gttacgtcaa tttataaatg
    13201 atattcgggg ataattatgg cgcagtcgaa actctatcca gttgtgatgg caggtggctc
    13261 cggtagccgc ttatggccgc tttcccgcgt actttacccc aagcagtttt tatgcctgaa
    13321 aggcgatctc accatgctgc aaaccaccat ctgccgcctg aacggcgtgg agtgcgaaag
    13381 cccggtggtg atttgcaatg agcagcaccg ctttattgtc gcggaacagc tgcgtcaact
    13441 gaacaaactc accgagaaca ttattctcga accggcaggg cgtaacactg cacctgccat
    13501 tgcactggcg gcgctggcgg caaaacgtca tagcccggag agcgacccgt taatgctggt
    13561 attggcggcg gatcatgtga ttgccgatga agacgcgttc cgtgccgccg tgcgtaatgc
    13621 catgccatat gccgaagcgg gcaagctggt gaccttcggc attgtgccgg atctacctga
    13681 aaccggttat ggctatattc gtcgcggtga agtgtctgcg ggtgagcagg atacggtggc
    13741 ctttgaagtg gcgcagtttg tcgaaaaacc gaatctggaa accgcccagg cctatgtggc
    13801 aagcggcgaa tattactgga acagcggcat gttcctgttc cgcgccggac gctatctcga
    13861 agaactgaaa aaatatcgcc cggatatcct cgatgcctgc gaaaaagcga tgagcgccgt
    13921 cgatccggat ctcgatttta ttcgcgtgga tgaagaagcg tttctcgcct gcccggaaga
    13981 gtcggtggat tacgcggtca tggaacgtac ggcagatgcc gttgtggtgc cgatggatgc
    14041 gggctggagc gatgtcggtt cctggtcttc attatgggag atcagcgccc acaccgccga
    14101 gggcaacgtt tgccacggcg atgtgattaa tcacaaaaca gaaaacagct atgtgtatgc
    14161 cgaatctggc ctcgtaacca ccgtcggggt gaaagatctg gtggtagtgc agaccaaaga
    14221 tgcggtgctg attgccgacc gtaacgcggt gcaggatgtg aaaaaagtgg tcgagcagat
    14281 caaagccgat ggtcgccatg agcatcgggt acatcgcgaa gtgtatcgtc cgtggggcaa
    14341 atatgactct atcgacgcgg gcgaccgcta ccaggtgaaa cgcatcaccg tgaaaccggg
    14401 cgaaggcttg tcggtacaga tgcaccatca ccgcgcggaa cactgggtgg tggtcgcggg
    14461 aacggcaaaa gtcactattg acggtgatat caaactgctt ggtgaaaacg agtccattta
    14521 tattccgctg ggggcgacgc actgcctgga aaacccgggg aaaattccgc tcgatttaat
    14581 tgaagtgcgc tccggctctt atctcgaaga ggatgatgtg gtgcgcttcg cggatcgcta
    14641 cggacgggtg taaacgtcgc atcaggcgct gtttgtcgga tgcggcgtga acgccttatc
    14701 cgacctacgg ttcggatttg taggcctgat aagacgcggc agcgtcgcat caggcaatga
    14761 atgcgaaacc gcgatgtaaa taacgacaaa aacaaaattg gccgtttcgg tcagggccaa
    14821 ctattgcctg aaaaagggta acgatatgaa aaaattaacc tgctttaaag cctatgatat
    14881 tcgcgggaaa ttaggcgaag aactgaatga agatattgcc tggcgcattg gtcgcgccta
    14941 tggcgaattt ctcaaaccga aaaccattgt gttaggcggt gatgtccgcc tcaccagcga
    15001 aaccttaaaa ctggcgctgg cgaaaggttt acaggatgcg ggcgtcgatg tgctggatat
    15061 tggcatgtcc ggcaccgaag agatctattt cgccacgttc catctcggcg tggatggcgg
    15121 cattgaagtt accgccagcc ataatccgat ggattataac ggcatgaagc tggttcgcga
    15181 gggggctcgc ccgatcagcg gagataccgg actgcgcgac gtccagcgtc tggctgaagc
    15241 caacgacttc cctcccgtcg atgaaaccaa acgcggtcgc tatcagcaaa tcaacctgcg
    15301 tgacgcttac gttgatcacc tgttcggtta tatcaacgtc aaaaacctca cgccgctcaa
    15361 gctggtgatc aactccggga acggcgcagc gggtccggtg gtggacgcca ttgaagcccg
    15421 ctttaaagcc ctcggcgcac ccgtggaatt aatcaaagtg cacaacacgc cggacggcaa
    15481 tttccccaac ggtattccta acccactact gccggaatgc cgcgacgaca cccgcaatgc
    15541 ggtcatcaaa cacggcgcgg atatgggcat tgcctttgat ggcgattttg accgctgctt
    15601 cctgtttgac gaaaaagggc agtttattga gggttactac attgtcggcc tgctggcaga
    15661 agcgttcctc gaaaaaaatc ccggcgcgaa gatcatccac gatcctcgtc tctcctggaa
    15721 taccgttgat gtggtgactg ccgcaggcgg caccccggta atgtcgaaaa ccggacacgc
    15781 ctttattaaa gaacgtatgc gcaaggaaga cgccatctac ggtggcgaaa tgagcgccca
    15841 ccattacttc cgtgatttcg cttactgcga cagcggcatg atcccatggc tgctggtcgc
    15901 cgaactggtg tgcctgaaag gaaaaacgct gggcgaactg gtgcgcgacc ggatggcggc
    15961 gtttccggca agcggtgaga tcaacagcaa actggcgcaa cccgttgagg cgattaaccg
    16021 cgtggaacag cactttagcc gtgaggcgct ggcggtggat cgcaccgatg gcatcagcat
    16081 gacctttgcc gactggcgct ttaatctgcg ctcctccaac accgaaccgg tggtgcggtt
    16141 gaatgtggaa tcgcgcggtg atgtaccgct gatggaagcg cgaacgcgaa ctctgctgac
    16201 gttgctgaac gagtaatgtc attactttcc ccctcaccct gacgctctac tgcctgcgga
    16261 gagaagtgag cacggaactt tcttctcccc ctcgcccctt tggggagagg gccggggtga
    16321 ggggcgaggc gacaatagaa caggaacaac gatgacaaat ctaaaaaagc gcgagcgagc
    16381 gaaaaccaat gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt
    16441 tgccggacta tggctggttt gcgaagtgag cggactgtca ttcctctaca tgcacctgtt
    16501 ggtggcgttg attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg
    16561 ctcatggcgc ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacctt
    16621 aagcgtgatt ttcagcgccg gactggtggc gttcaataat gatttcgaca cgcaactgaa
    16681 aatctggctg gcgtggtatg ggctgaccag catcggactg gtggtttgcc gttcatgtat
    16741 tcgcattggc gcgggctggc tgcgtaatca tggctataac aagcgtatgg tcgccgtagc
    16801 gggggattta gccgccggac aaatgctgat ggagagcttc cgtaatcagc cgtggttagg
    16861 gtttgaagtg gtgggcgttt accacgaccc gaaaccgggt ggcgtttcta acgactgggc
    16921 gggcaatctg caacagctgg tcgaggatgc gaaagcgggc aagattcata acgtctatat
    16981 cgcgatgcaa atgtgcgacg gcgcgcgagt gaaaaaactg gtccatcaac tggcggacac
    17041 tacctgttcg gtgctgctga tccccgatgt ctttaccttc aacattctcc attcccgtat
    17101 tgaagagatg aacggcgtac cagtggtgcc gctgtatgac acgccgcttt ccggggttaa
    17161 ccgcctgctc aaacgtgcag aagacattgt gctggcaacg ctgattctgc tgctgatctc
    17221 cccggtgctg tgctgcattg cgttggcggt gaaactgagc tcaccggggc cggttatttt
    17281 ccgccagact cgctacggca tggacggcaa gccgatcaaa gtgtggaagt tccgttcgat
    17341 gaaagtgatg gagaacgaca aagtggtgac tcaggcgacg cagaacgatc cgcgcgtcac
    17401 caaagtgggg aactttctgc gccgcacctc gctggacgaa ttgccgcagt ttatcaatgt
    17461 gctgaccggg gggatgtcga ttgtcggtcc acgtccgcac gcagtagcgc ataacgaaca
    17521 gtatcgccag ctcattgaag gctacatgct gcgccacaaa gtgaaaccgg gcattaccgg
    17581 ctgggcgcag attaatggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg
    17641 cgtcgagttc gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt
    17701 tttcctgacg gtattcaaag gcttcgttaa caaagcggca tattgatatg agcttacgtg
    17761 aaaaaaccat cagcggcgcg aagtggtcgg cgattgccac ggtgatcatc atcggcctcg
    17821 ggctggtgca aatgactgtg ctggcgcgga ttatcgacaa ccaccagttc ggtttgctga
    17881 ctgtgtcgct ggtgattatc gcgctggcag atacgctttc tgacttcggt atcgctaact
    17941 ccattattca gcgaaaagaa atcagccacc ttgaactcac cacgttgtac tggctgaacg
    18001 tcgggctggg gatcgtggcg tgtgtggcgg tatttttgtt gagtgatgcc attggcgaca
    18061 tgctgaataa cccggacctg gcaccgttga ttaaaacatt gtcactagcg tttgtggtga
    18121 tcccccacgg gcaacagttc cgcgcgttga tgcaaaaaga gctggagttc aacaaaatcg
    18181 gcatgatcga aaccagcgcg gtgctggcgg gctttacttt tacggtggtt agcgcccatt
    18241 tctggccgct ggcgatgacc gcgatcctcg gttatctggt caatagtgcg gtgagaacgc
    18301 tgctgtttgg ctactttggc cgcaaaatct atcgccccgc tctgcatttc tcgctggcgt
    18361 cggtggcacc gaacttacgc tttggtgcct ggctgacggc ggacagcatc atcaactatc
    18421 tcaacaccaa cctttcaaca ctcgtgctgg cgcgtattct cggcgcgggc gtggctgggg
    18481 gatacaacct ggcgtacaac gtggccgttg tgccgccgat gaaactgaac ccgatcatca
    18541 cccgcgtgtt gtttccggcg tttgccaaaa ttcaggacga taccgaaaag ctgcgcgtca
    18601 acttctacaa gctgctgtcg gtagtgggga ttatcaactt tccggcgctg ctggggctga
    18661 tggtggtgtc gaataacttt gtaccgctgg tatttggcga aaagtggagc agcattattc
    18721 cggtgctaca gctgctgtgt gtggtgggtc tgctgcgctc cgtgggtaac ccgattggtt
    18781 cgctgctgat ggcgaaagcg cgggtcgata tcagctttaa attcaacgta tttaaaacat
    18841 ttctgtttat tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc
    18901 ttggcttcct gctggtgcag attatcaaca ctattctgag ctatttcgtg atgattaaac
    18961 cggttcttgg ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttatctct
    19021 cgctgccaac gctggtggtc agttatgcgc tgggcattgt gctgaaaggg caactggcgc
    19081 tggggatgct gctggcggtg caaatagccg cgggtgtgct ggcgtttgtg gtgatgattg
    19141 tgctgtcgcg ccatccgctg gtggtggaag tgaagcgtca gttttgtcgc agcgaaaaaa
    19201 tgaaaatgct tttacgggcg gggtgagttg ttgttttcgg ttccgacaac ggttgccggg
    19261 tgcagcgtaa aacgccttat ccgtcctacg gttctgtgct cgcatcaggc aaaaaacggc
    19321 aacgaacaca acaaacacag cctcacctgg catcaaattt atgaggaaaa aatgaaatta
    19381 cttattctgg gcaaccacac ttgcggcaat cgtggcgaca gcgccatctt gcgcggctta
    19441 cttgatgcca tcaacatcct caatccacat gccgaagtgg acgtgatgag ccgctatcct
    19501 gtcagttctt cctggctact caaccgcccg gtaatgggcg atccgctgtt cctgcaaatg
    19561 aaacaacaca acagcgcggc gggcgttgtc gggcgcgtta aaaaagtcct ccgtcgccgc
    19621 taccagcatc aggtattgct ctcacgcgtc accgacagtg gcaagctgcg taatatcgcc
    19681 atcgcccagg gattcaccga cttcgtgcgc ctgctgtcag gttacgacgc cattattcag
    19741 gtcggcggat cgttttttgt cgatctctac ggcgtgccac agtttgaaca tgcactttgc
    19801 acgtttatgg cgaaaaagtc gctgtttatg attggtcaca gtgttggccc gttccaggat
    19861 gagcaattta accaactggc gaactacgtt tttggtcact gcgacgcgct gatcctgcgc
    19921 gaatcggtca gccttgaact gatgaaacgc agcaatatca ccacttcaaa agttgaacat
    19981 ggcgtcgata ccgcgtggct ggtcgatcac cacacagaag acttcaccgc cagctatgct
    20041 gttcaacact ggctggacgt tgccgcacaa cagaaaacgg tggcaattac cctgcgcgaa
    20101 ctggcaccgt tcgacaaacg tctcggcacc actcaacaag catatgaaaa agcctttgcc
    20161 ggggtggtca atcgcattct cgacgaaggc tatcaggtga ttgcgctctc cacctgtacg
    20221 ggtatcgaca gctataacaa agacgaccgc atggtggcgc tcaacctgcg ccagcacatc
    20281 agcgatcctg cccgttacca cgtagtgatg gatgaactca acgatctgga aatgggcaaa
    20341 attctggggg cctgtgaact caccatcggt acgcgcctgc actccgccat tatctcaatg
    20401 aactttgcca ccccggcgat tgccatcaat tatgaacata aatccgccgg gattatgcag
    20461 cagctgggac taccggagat ggcgattgat atccgtcatt tattagacgg cagcctgcaa
    20521 gtgatggttg cggatacctt aggccagctt ccggcactga acacgcgact taacgaagcc
    20581 gtcagtcgcg aacgtcagac gggaatgcag atggtgaaat ctgtgcttga acacatcagg
    20641 gaggcgaaat gaaggtcggc ttctttttac tgaaatttcc gctgtcgtcg gaaaccttcg
    20701 tcctcaatca aattaccgcg tttattgata tgggctttga ggtggagatt gtcgcgctgc
    20761 aaaaaggcga cacccagaac acccacgcgg catggacgaa atacaacctt gccgccagaa
    20821 cccgttggtt acaggacgaa cctacgggca aagtggcgaa actgcgccac cgtgccagcc
    20881 agatcttacg cggcattcat cgtaaaaata cctggcaggc acttaacctc aaacgctatg
    20941 gtgccgagtc gcgaaacctg attttgtctg ccatttgcgg ccaggtcgca acaccatttt
    21001 atgccgatgt ctttatcgct cattttggcc ctgcgggggt aaccgcagca aaactccgcg
    21061 aactgggtgt cattcgcggc aaaattgcca ctatcttcca cggcattgat atctccagtc
    21121 gggaggtgct caaccattac actcccgaat atcagcaact gttccgccgt ggcgatctga
    21181 tgctaccaat aagcgatctg tgggccggaa ggttgcaaaa aatgggctgc ccgagggaaa
    21241 aaatcgccgt atcgcgcatg ggcgtggaca tgacgcgttt tagcccgcgt ccggtgaaag
    21301 cgcccgcaac gccgctggaa atcatttccg tcgcacgctt aaccgagaaa aaaggcctgc
    21361 atgtggcgat tgaagcctgc cgtcagttga aagagcaggg cgtggcattt cgctatcgca
    21421 tcctcggcat tggcccgtgg gaacgacgcc tgcgcaccct catcgaacaa tatcaactgg
    21481 aagatgtggt agagatgccg ggctttaaac cgagccacga agtgaaagcg atgctcgacg
    21541 acgcggatgt cttcctgttg ccatcggtta caggtgcgga tggcgatatg gaaggtattc
    21601 cggtggcact aatggaagcg atggcggtcg gcattccggt ggtttctact ctgcatagcg
    21661 gaataccgga actggtggag gccgataaat ccggctggct ggtgcctgag aacgatgctc
    21721 gcgcactggc gcaacgactg gcggcgttta gccaactgga caccgacgaa ctggctccgg
    21781 tcgtcaaacg tgcgcgcgaa aaagtcgaac acgattttaa ccagcaggtg atcaatcgag
    21841 aactcgccag cttgctgcag gctttataga ggagaacgca tgccatttaa aaaactctcc
    21901 cgacgcacct tcctgacggc aagctcggtg cttgccttcc tccatacccc tttcgctcgc
    21961 gcgcttcccg cccgacaaag cgttaacatt aacgactaca accctcgcga ctggatcgcc
    22021 tcatttaaac aagccttcag cgaagggcaa acggtcgtcg tgcctgccgg attggtttgc
    22081 ctcaatatca acaccggcat ctttatccct cctggcaaaa cgttacacat ccttggaagc
    22141 ctgcgcggca acggcagagg gcgatttgtc ttacaggacg gcagccaggt gacaggggag
    22201 gagggcggca gtatgcataa catcaccctg gatgtgcgcg gctctgactg caccatcaaa
    22261 gggctggcga tgagcggctt tggtccggta acgcagattt atatcggcgg caaaaacaaa
    22321 cgggtcatgc gtaacctgac catcgataac ctgaccgtta gccacgctaa ttacgccatc
    22381 ttacgccagg gatttcataa ccagattatc ggtgccaaca tcaccaattg taagttcagc
    22441 gacttacagg gcgacgccat tgaatggaat gtggcgatta acgaccgtga tattttgata
    22501 tctgaccatg tcatcgagcg catcaactgt actaacggca aaatcaattg gggcatcggc
    22561 ataggtcttg cgggaagcac ttatgataac aactatccgg aagaccaggc agtgaaaaac
    22621 tttgtcgtgg cgaacatcac gggatcggat tgtcggcagt tgatacatgt tgaaaatggt
    22681 aaacattttg ttattcgtaa tatcaaagcc tgcaatatca cgccggattt cagtaagaaa
    22741 gcaggtattg ataacgccac ggtcgctatt tacggttgtg acaatttcgt gattgataat
    22801 attgaaatga ttaatagcgc cgggatgtta atcggctatg gggtaattaa aggcaaatat
    22861 ctctcgatac cgcagaattt ccgagtgaat aatattcagc tggataatac ccaccttgct
    22921 tacaaattgc gcggcatcca aatctccgcc gggaatgctg tctcctttgt ggcgctgact
    22981 aacgttgaga tgaagcgtgc gtcgctggag ttacacaaca agccgcaaca cctttttatg
    23041 cgtaatatca aagtgatgca ggaatcctct gttggacccg cattgagcat gaacttcgac
    23101 atgcgcaaag acgttcgcgg cgtgtttatg gcgaaaaaag aaacactgct atctcttgca
    23161 aatgttcatg cggtgaatga aagagggcaa agctccgtcg atatcgacag gataaatcac
    23221 catattgtta atgtggaaaa gattaacttt agattgccgg aacggaggga gtagatttgc
    23281 gaccattcct ggaaaaatgg agccatactt aggaacaatg ctactgcaat ctacaacgaa
    23341 gcggcgtaac atcacaagta attcagtaat caattcaggg taattgatgc tggcgaaaaa
    23401 aatcgaacaa gctataattc agcaaccatt ttacaggtgg atgaaataat gacgaattta
    23461 aaagcagtta ttcctgtagc gggtcttggg atgcatatgt tgcctgccac taaggcgatt
    23521 cccaaagaga tgctaccgat cgtcgacaag ccaatgattc agtacattgt tgacgagatt
    23581 gtggctgcag ggatcaaaga aatcctcctg gtaactcatg cgtccaagaa cgcggtcgaa
    23641 aaccacttcg acacctctta tgaattagaa tctcttcttg aactgcgcgt gaagcgtcaa
    23701 ctgctggcgg aagtacagtc catctgtccg ccaggcgtga ccattatgaa cgtgcgtcag
    23761 ggcgaacctt taggtttagg ccactccatt ttgtgtgcac gacccgccat tggtgacaat
    23821 ccatttgtcg tggtactgcc ggacgttgtg atcgacgacg ccagtgccga cccgctgcgc
    23881 tacaaccttg ctgccatgat tgcgcgtttc aatgaaacgg gccgtagcca ggtgctggca
    23941 aaacgtatgc cgggtgagct ctctgaatac tctgtcatcc agaccaaaga accgctggat
    24001 cgcgaaggta aagtcagccg cattgttgaa tttatcgaaa aaccggatca gccacaaacg
    24061 ctggactcag acatcatggc cgttggtcgc tatgtacttt ctgccgatat ttggccggaa
    24121 ctggaacgta ctcagcctgg tgcatgggga cgtattcaac tgactgatgc tattgccgag
    24181 ctggcgaaaa aacagtccgt tgatgccatg ctgatgactg gggacagtta cgactgcggt
    24241 aaaaaaatgg gctatatgca ggcgttcgtg aagtatggat tacgaaacct gaaagaaggg
    24301 gcgaagttcc gtaaaggcat tgagaagctg ttaagcaaat aatgaaaatc tgaccggatg
    24361 taacggttga taagaaaatt ataacggcag tgaagattcg tgatgaaagt aatttgttgc
    24421 gaatattcct gccgttgttt tatataaaca atcagaataa caacgagtta gcaataggat
    24481 tttagtcaaa gttttccagg attttccttg tttccagagc ggattggtaa gacaattagc
    24541 gtttgaattt ttcgggttta gcgcgagtgg gtaacgctcg tcacatcgta gacatgcatg
    24601 cagtgcactg gtagctgtaa agccaggggc ggtagcgtgc attaataccc ctattaatca
    24661 acctaagagc cgcttatttc acagcatgct ctgaagtaat atggaataat aaagtgaaga
    24721 tacttgttac tggtggcgca ggatttattg gttctgctgt agttcgtcac attataaata
    24781 atacgcagga tagtgttgtt aatgtcgata aattaacgta cgccggaaac ctggaatcac
    24841 ttgctgatgt ttctgattct gaacgctatg tttttgaaca tgcggatatt tgcgatgcag
    24901 ctgcaatggc gcggattttt gctcagcatc agccggatgc agtgatgcac ctggctgctg
    24961 aaagccatgt tgaccgttca attaccggcc ctgcggcatt tattgaaacc aatattgttg
    25021 gtacttatgt ccttttggaa gccgctcgca attactggtc tgctcttgat agcgacaaga
    25081 aaaatagctt ccgttttcat catatttcta ctgacgaagt ctatggtgat ttgcctcatc
    25141 ctgacgaagt aaataataca gaagaattac ccttatttac tgagacgaca gcttacgcgc
    25201 caagcagtcc ttattccgca tccaaagcat ctagcgatca tttagtccgc gcgtggaaac
    25261 gtacctatgg tttaccgacc attgtgacta actgttcgaa taactacggc ccttatcact
    25321 ttccggaaaa attgattccg ctggtaattc ttaatgctct ggaaggtaag gcattaccta
    25381 tttatggcaa aggggatcaa attcgcgatt ggctatatgt tgaagatcat gcgcgtgcgt
    25441 tatataccgt tgtcaccgaa ggtaaagcgg gtgaaactta taacattggc ggacacaacg
    25501 aaaagaaaaa catcgatgtt gtgctgacta tttgtgattt gttggatgag attgtaccga
    25561 aagagcaatc ttatcgtgag caaattactt atgttgccga tcgtccggga cacgatcgcc
    25621 gttatgcgat tgatgctgag aagattagcc gcgaattggg ctggaaaccg caggaaacgt
    25681 ttgagagcgg gattcggaag acagtggaat ggtacctgtc caatacaaaa tgggtcgaaa
    25741 atgtgaaaag tggtgcctat cagtcatgga ttgcacagaa ctatgagggc cgtcagtaat
    25801 gaatatcctc cttttcggca aaacagggca ggtaggttgg gaactacagc gtgctctggc
    25861 acctctgggt aatttgattg ctcttgatgt tcactctact gattattgcg gtgattttag
    25921 taatcctgaa ggtgtagctg aaaccgtaag aagcattcgg cctgatatta ttgtcaacgc
    25981 agccgctcac accgcagtag acaaagcaga atcagaaccg gagtttgcac aattacttaa
    26041 cgcaacaagt gtcgaagcga ttgcgaaagc agccaatgaa gtcggcgcct ggcttattca
    26101 ttactcgact gattacgtct tccctggaaa tggcgatatg ccatggcggg agacggatgc
    26161 aaccgcacca ctaaatgttt acggtgaaac caagttagcc ggagaaaaag cgttacagga
    26221 atattgcgca aagcatctta ttttccggac cagctgggtc tatgcaggaa aaggaaataa
    26281 cttcgccaaa acgatgttac gtctggcaaa agagcgtgaa gaattagcgg ttattaatga
    26341 tcagtttggt gcgccaacag gtgctgaact gctggctgat tgtacagcac atgccattcg
    26401 tgtcgcactg aataaaccag aagtcgcagg cttgtaccat ctggtagcca gtggtaccac
    26461 aacctggcac gattatgctg cgctggtttt tgaagaggca cgcaaagcag gtattcccct
    26521 tgcactcaac aagctcaacg cagtaccaac aacagcctat cctacaccag ctcgtcgtcc
    26581 gcataactct cgccttaata cagaaaaatt tcagcagaaa tttgcgcttg ttttgcctga
    26641 ttggcaggtt ggcgtgaaac gaatgctcaa cgaattattt acgactacag caatttaata
    26701 gtttttgcat cttgttcgtg atggtggagc aagatgaatt aaaaggaatg atgaaatgaa
    26761 aacgcgtaaa ggtattattt tagctggtgg ttcgggtact cgtctttatc ctgtaactat
    26821 ggctgtcagt aaacagttgt taccgattta tgataaaccg atgatctatt acccgttgtc
    26881 tacactgatg ttagcgggtc ttcgcgatat tctgattatt agtacgccac aggatactcc
    26941 tcgttttcaa caactgctgg gtgacgggag ccagtggggg ctaaatcttc agtacaaagt
    27001 gcaaccgagt ccagatggtc ttgcgcaggc atttatcatc ggtgaagagt ttatcggtgg
    27061 tgatgattgt gctttggttc taggtgataa tatcttttac ggtcacgatc tgccgaagtt
    27121 aatggatgtc gctgttaaca aagaaagtgg tgcaacggta tttgcctatc acgttaatga
    27181 tcctgaacgc tacggtgtcg ttgagtttga taaaaacggt acggcgatca gcctggaaga
    27241 aaaaccgcta caaccaaaaa gtaattatgc ggtaaccggg ctttattttt atgataacta
    27301 cgttgtcgaa atggcgaaaa atcttaagcc ttctgcccgc ggtgaactgg aaattaccga
    27361 tattaaccgt atttatatgg aacaggggcg tttatccgtt gccatgatgg gacgtggtta
    27421 tgcatggctg gacacgggga cacatcaaag tcttattgag gcaagcaatt ttatcgcaac
    27481 aatagaagaa cgtcaggggc tgaaagtttc ctgcccggaa gaaattgctt accgtaaagg
    27541 gtttatcgat gctgagcagg tgaaagtatt agctgaaccg ttgaagaaaa atgcttatgg
    27601 tcagtatctg ctgaaaatga ttaaaggtta ttaataaaat gaacgtaatt aaaacagaaa
    27661 ttccagatgt actgattttt gaaccgaaag tttttggtga tgagcgtggt ttctttatgg
    27721 aaagctttaa tcagaaagtt tttgaagaag ctgtagggcg gaaggttgaa tttgttcagg
    27781 ataatcattc taaatcatct aagggtgtgt tacgcggact gcactatcag ctggaaccct
    27841 atgctcaagg taaattagtt cgttgtgtgg tcggtgaagt atttgatgta gcagttgata
    27901 ttcgtaaatc gtcacctaca tttgggaaat gggttggggt gaatttgtct gctgagaata
    27961 agcgtcagtt gtggatccct gaggggtttg cgcatggttt tttggtgtta agcgagatag
    28021 cagagtttgt ttataagacg acaaattatt atcatcctga atcagaaggt tgcataaaat
    28081 gggatgattc ttttttgatg attgattggc caaataaacc gatagcaatc tcagaaaaag
    28141 ataaaaaagg ctcatcaatt ttgaggctaa atgactattg atgttgaaaa aaaaacttca
    28201 aaaaataaag gaatatcatt cagtattgga gttggcaata attcagggtg cgaatgccat
    28261 atttcctgtg ttggtattcc cattttttct tattacctta ggggaaaaca tcttttcaag
    28321 tattgctgtt ggtgaagtac tagcactata tgtgcttata ttttcgctat acagttttga
    28381 tattataagt gtgcagaagg taatttcaag tgtgacaaaa gatgaaatat ttaaagttta
    28441 cattctgaca ctaatctgta ggttgt
//
LOCUS       DAZFKM010000059        28267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFKM010000059 DAZFKM010000000
VERSION     DAZFKM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..1140)
                     /gene="gldA"
                     /locus_tag="ACHKAY_002571"
     CDS             complement(37..1140)
                     /gene="gldA"
                     /locus_tag="ACHKAY_002571"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO5701235.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1151..1813)
                     /gene="fsa"
                     /locus_tag="ACHKAY_002572"
     CDS             complement(1151..1813)
                     /gene="fsa"
                     /locus_tag="ACHKAY_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO5701236.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1825..4326)
                     /gene="ptsP"
                     /locus_tag="ACHKAY_002573"
                     /pseudo
     CDS             complement(1825..4326)
                     /gene="ptsP"
                     /locus_tag="ACHKAY_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4635..5714
                     /gene="frwC"
                     /locus_tag="ACHKAY_002574"
     CDS             4635..5714
                     /gene="frwC"
                     /locus_tag="ACHKAY_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO5701237.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5729..6049
                     /gene="frwB"
                     /locus_tag="ACHKAY_002575"
     CDS             5729..6049
                     /gene="frwB"
                     /locus_tag="ACHKAY_002575"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5701238.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6100..8397
                     /gene="pflD"
                     /locus_tag="ACHKAY_002576"
     CDS             6100..8397
                     /gene="pflD"
                     /locus_tag="ACHKAY_002576"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5701239.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8363..9241
                     /gene="pflC"
                     /locus_tag="ACHKAY_002577"
     CDS             8363..9241
                     /gene="pflC"
                     /locus_tag="ACHKAY_002577"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO5701240.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9243..9584
                     /gene="frwD"
                     /locus_tag="ACHKAY_002578"
     CDS             9243..9584
                     /gene="frwD"
                     /locus_tag="ACHKAY_002578"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO5701241.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9571..10422)
                     /gene="yijO"
                     /locus_tag="ACHKAY_002579"
     CDS             complement(9571..10422)
                     /gene="yijO"
                     /locus_tag="ACHKAY_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO5701242.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10571..12304)
                     /gene="eptC"
                     /locus_tag="ACHKAY_002580"
     CDS             complement(10571..12304)
                     /gene="eptC"
                     /locus_tag="ACHKAY_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5701243.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12487..15138)
                     /gene="ppc"
                     /locus_tag="ACHKAY_002581"
     CDS             complement(12487..15138)
                     /gene="ppc"
                     /locus_tag="ACHKAY_002581"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO5701244.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15490..16641)
                     /gene="argE"
                     /locus_tag="ACHKAY_002582"
     CDS             complement(15490..16641)
                     /gene="argE"
                     /locus_tag="ACHKAY_002582"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO5701245.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16795..17799
                     /gene="argC"
                     /locus_tag="ACHKAY_002583"
     CDS             16795..17799
                     /gene="argC"
                     /locus_tag="ACHKAY_002583"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO5701246.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17807..18583
                     /gene="argB"
                     /locus_tag="ACHKAY_002584"
     CDS             17807..18583
                     /gene="argB"
                     /locus_tag="ACHKAY_002584"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO5701247.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18644..20017
                     /gene="argH"
                     /locus_tag="ACHKAY_002585"
     CDS             18644..20017
                     /gene="argH"
                     /locus_tag="ACHKAY_002585"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO5701248.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20284..21201
                     /gene="oxyR"
                     /locus_tag="ACHKAY_002586"
     CDS             20284..21201
                     /gene="oxyR"
                     /locus_tag="ACHKAY_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO5701249.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVEQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21184..22584)
                     /gene="sthA"
                     /locus_tag="ACHKAY_002587"
     CDS             complement(21184..22584)
                     /gene="sthA"
                     /locus_tag="ACHKAY_002587"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO5701250.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22915..23565
                     /gene="fabR"
                     /locus_tag="ACHKAY_002588"
     CDS             22915..23565
                     /gene="fabR"
                     /locus_tag="ACHKAY_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO5701251.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23565..23924
                     /gene="yijD"
                     /locus_tag="ACHKAY_002589"
     CDS             23565..23924
                     /gene="yijD"
                     /locus_tag="ACHKAY_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO5701252.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23964..25064)
                     /gene="trmA"
                     /locus_tag="ACHKAY_002590"
     CDS             complement(23964..25064)
                     /gene="trmA"
                     /locus_tag="ACHKAY_002590"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO5701253.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25178..25368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25433..27277
                     /gene="btuB"
                     /locus_tag="ACHKAY_002591"
     CDS             25433..27277
                     /gene="btuB"
                     /locus_tag="ACHKAY_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO5701254.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27222..28079
                     /gene="murI"
                     /locus_tag="ACHKAY_002592"
     CDS             27222..28079
                     /gene="murI"
                     /locus_tag="ACHKAY_002592"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO5701255.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6459 a         7107 c         7795 g         6906 t
ORIGIN      
        1 ttcaaactcc cggacaagcc gggagtttgg agtaggttat tcccactctt gcaggaaacg
       61 ctgaccgtac tggtcggcta ccagcagagc ggcgtaaacc tgatctggcg tcgcgccgcc
      121 aggcatgttg tggatggttt caccttctgc acatgccgct tctgccacaa ttcgcatttt
      181 cgccgggaca tcttctttaa tatccagttg agcgagagtt attggcaaac ctaccgcatg
      241 gctaagtgca gctacggttt cgatttcctc aaccggcgcg ttttccagta ccagctgcgt
      301 cagcgtaccg aatgccactt tttcaccgtg ataatagtga tgcgcgtccg ggatagcggt
      361 caggccgtta tgcaccgcat gagccgccgc cagtccgccg ctttcaaaac caacgccgct
      421 taaataggtg ttcgcttcaa tcacgcgttc cagcgcaggg gttactacat gctgttcggc
      481 ggcaagcatc gctttttcgc cttcttccag cagagtgttg tagcacagtt ctgccagtgc
      541 cagcgccgcc tgggtgcact tgccgcccgc catagtggta gcgccgctac gggaacaggc
      601 gcgtgcttca aaccaggttg ccagcgcatc gccgataccc gccgctaaca gacgtgcagg
      661 agcgcccgcg acgattttgg tgtcgacaat gaccatattc gggttatttg gcaacagtag
      721 atagcggtca aactcaccct catcggtgta gataacagac aatgcgctgc acggtgcatc
      781 ggtagaggcg atagtcggtg cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt
      841 ggcagtatcg agggtttttc cgccaccgat accgagaatt gcgccacact gcgcagtctc
      901 cgcgatgcca cgcagacgat cgatctcatt ttgcgaacat tcaccgccaa acggcgcaat
      961 ttctactacc agtccagcat ctttaaagct tttctcgaca gtggattgag caaaacctaa
     1021 aacaaatttg tcacccacca ctaaccagcg ttctgccagc ggcttcaggt attcgcccag
     1081 acgattaatc acatcagcgc cctggatgta tttacccggt gattgaataa tgcggtccat
     1141 aattgctcct ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt tcgaacttct
     1201 ctatagctga ctctaccgca ggggtgttga gcatttgttg cgctacatct aaaggcaggg
     1261 tgatggactc acatcctgcc agcaaacaat ccagtgcctg acgcggcgtt ttaaagctgg
     1321 ctgcaagcac catgctttct ggcgcgtgca tttctaacag cgcttgcagc tcctgaaccg
     1381 tacgaatgcc gtccccgccc tgggcatcta cgcggttaac atacggagca acgtattttg
     1441 cccctgccag cgcggccagt aacccttgtg cagcactgta cactgcggtg ccaagcgtag
     1501 taataccctc ttttttcagc atcttaatgg cggccagacc ttcggaagtc accgggattt
     1561 tcaccacaat gcccggaatg gcgtcgcgca ggcgcttcgc ttcttccacc ataccctgcg
     1621 cgtcacgact catagtctga gcaaacagaa tgccctcatc accaatcgct ttttgcagac
     1681 gcggcagaac ttcccatatg gactccttgc tggcagcgat aatgctcggg ttagttgtca
     1741 ccccggcaat ggggaatatg cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca
     1801 gatacagttc catgatgttt ccttttacag ttccagttca tgttgcagca ggctggcgat
     1861 agcgtcggca gaggcggcgt taaccagcgc gttacggaat tcttcgtgca tgatgcggcg
     1921 agcgaggcgc gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt tcagggttaa
     1981 cataatgatg aattgcgctt catcatcgcc ccagcgcacc ggcgcttgca gacgcgctac
     2041 actgatggtg gattgctcaa tgtgttctga tttgctgtgg ggaatggcaa aactaaagcc
     2101 cagaccggta gagaaaacgg cctcgcgcgc ccacaagtcg gcttccagtt tacgcggata
     2161 gcggcagcgg cccgccagca gcaggttatc ggtcatgcct ttgagcactt cttctttgct
     2221 gcgtcagtcg ctttccagcg tgatgcactc ggcggtgacc agcggtgcgt cctgttgggt
     2281 catgcggaat tgcgccagca ggtgttccac ttccagcgaa gtacggcagg ccattgcctg
     2341 gttgagcaac tggcggcatt cgcggctatc aagttgcgcc attcgcgctt tcgccgccgg
     2401 aattgacggt gcgctcatgc tcagctcatc cagccctaaa ccgaccagca acggcagcac
     2461 ggaacctttc gctcccagct caccgcacag accaatccat ttgccctggc gatgcaccgc
     2521 ttgcacggcg taatcgagcg cccgcaagaa tgccggattc aggctgttgt agtgacgagt
     2581 aaccttagca ttatcgcgat ccaccgccag cagatattgc gtcaggtcat tactaccaat
     2641 actaaagaaa tcaatctctt cgcagcattg atcgatgatg aacatcaccg acggcacttc
     2701 cagcataatg ccgagctgga tcttctcatc aaacggaatg tgttcgttac gcagttgctg
     2761 tttggcttcc gccagttttt ctttcaccca taagatctct tccattgaag agatcatcgg
     2821 gatcatgatt ttcaggctgc cgtgagcgga ggcgcgcagg atcgaccgta gttgtgtggt
     2881 gaacagcgag gcgtattctt cataaatacg cacagcgcga taaccgagga acgggttagc
     2941 ttctgccgga atgttcagat aatcaacggg tttgtcgccg ccaatgtcca tagtgcgcac
     3001 aataatgctg cgtccgttgg cagactccag cgcctgacaa aaaatgttgt acagctcgct
     3061 ttcgcccggt gcactggtgc gatccatata gagcatttca gtgcggaaca aaccaacgcc
     3121 ttccgcacca ttgccgaatg cggcctgcgc ttccacggag tgagcgatgt tagcggcaat
     3181 ttcaatgcgg ataccgtcag cggtacgggc ttgttgggtc agccagacac gctgttgctc
     3241 acgcagggca tcctgtacgc gggcttcttg ctgataataa cgggctacgg cttcccctgg
     3301 ctcaaccaca atcgctccgg cgttgccgtc gatataaatc gtttgatgtt gccacggggt
     3361 aagggcatca atatccacac caaccagcgt cggaatgttg aacgaacggg caaggatcac
     3421 cgtatgtgag gtggtgccgc cgcttttgag caacaatcct ttgaggtgat ttttatccag
     3481 ttcgaggaac tggctggggg tcagttcatc agccatacaa atggcgggct gcgtcagttt
     3541 gcccggtgcc gggaagcgtt gctcaccgta gatttgctgg agtaactgga agcagacgtc
     3601 gcgtacgtcc agggcacgtt cttgcaggta gctgctgctg gaacgggaaa actcttcaca
     3661 aaagtgattc gcactggcga caatcgcttc ggcgcagctt aatccggcgc tgacgcctgc
     3721 cagtaaatgt tcgtgcaggg aagtatcgcc agccagggat cggtgcgctt ccagaatcgc
     3781 gctggtggca ccgtcgctat ccagcagacg aaactcaatg tttttcagta ccagcgtcag
     3841 gccgttttcc agtgcggatt gctcggcgtc aacgtctttg gctgcgggaa gattactcag
     3901 cgcattgaga tctaaagaag agatcggcgt cagaatgccg cctgcgctac cgctgcacac
     3961 ggtgcgggcg cggataattt gcggattcag attggtcagt gaaaccggca gtggttccag
     4021 ttcgtcagat ttaacttccg ccagcggtgc gtcgcagtgg gggaattcat cgcgcagcca
     4081 ttggcttaaa cgctggtgcg cttcctgttc gtcggccccg gaaatcagta accggtagtt
     4141 atcgcccgcc agcgtgtcgg tgccaatcag cgcaagggcg cttttggcgt tgcccttgcg
     4201 gtcagtgcgc aggttatgcc actcaatttg tgatgaaaaa gtattacaca gcgtttcaac
     4261 gtggcttgcc ggacgtgcat gtacgccgtt aggtagctca caaataaatt ccacaatcag
     4321 ggccattgcc tctctcccgg tacggttttc tgtttacagg ataagagcgc acggcaacgg
     4381 cctgccatgt gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt caatttgcga
     4441 cgcgtctcac aagacgccgt tttgcggcat gcttccggat tatcgcaagt tatgaggcgg
     4501 atcgcatttt tgtactgata ttacaaaagt ccagtaaatg gcctttttat ccactgtttg
     4561 ctccgcctgc gattgcctat tgtttcctcc atcaaaaata tggacatggg ctttcgcaag
     4621 cccgggagca gcttatgaat gagttggtgc agatcctgaa aaatacccgt cagcatttaa
     4681 tgacgggcgt ttcacacatg attcccttcg tggtatcggg cggtattttg ctggcggttt
     4741 ccgtcatgtt gtatggcaaa ggcgcagtgc cggatgccgt agccgatccg aatctgaaaa
     4801 aactgtttga tatcggagtt gcaggtttga cgctgatggt gcctttcctc gccgcttaca
     4861 tcggttactc cattgcagag cgttctgcgc tggccccgtg cgctatcggt gcctgggttg
     4921 gtaacagctt tggtgcgggc ttctttggtg cgctgatcgc cgggattatc ggcggcatcg
     4981 tggtgcatta cctgaagaaa attccggtgc ataaagttct gcgctcggtg atgcctatct
     5041 tcattattcc tatcgtcggc acactgatta ccgcaggcat catgatgtgg ggcttgggcg
     5101 agcctgtagg ggcgttgacc aacagcctga ctcagtggct tcaggggatg cagcagggca
     5161 gcattgttat gctggcggtg atcatgggtc tgatgctggc gttcgatatg ggcggtccgg
     5221 ttaacaaagt ggcctatgcc ttcatgctga tttgcgttgc tcagggtgtt tataccgtgg
     5281 tggccattgc tgccgtgggt atttgtatcc cgccgctggg gatggggctg gcgacgctga
     5341 ttggtcgtaa aaatttctcc gcagaagagc gcgaaaccgg caaagcggcg ctggtgatgg
     5401 ggtgcgttgg ggttactgaa ggggcgattc ctttcgccgc tgccgatccg ctgcgtgtca
     5461 ttccttccat catggtcggt tctgtttgtg gtgcggtaac tgcggcgctg gtcggtgcgc
     5521 agtgctatgc aggctggggt ggtctgattg tgctgccggt ggttgaaggc aagctgggtt
     5581 atatcgcagc agtggctgtc ggagcagtgg tgacggctgt ttgcgtgaac gtgctgaaaa
     5641 gtctggcgcg taaaaatggg tcttcgactg atgagaagga agacgacctg gatttggatt
     5701 ttgaaattaa ttaattgagg aacgagccat gacgaaaatt attgcagtaa ccgcgtgtcc
     5761 atctggtgtt gcccatacct atatggcggc agaggcgctg gaaagcgccg cgaaagcgaa
     5821 aggctgggaa gtgaaagtag aaactcaggg gtcaattggt ctggaaaacg aactgactgc
     5881 ggaagacgtg gcgagcgccg atatggtgat tctgaccaaa gatatcggca tcaagtttga
     5941 agagcgtttt gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcgg taaaacgtgc
     6001 tgacgccatt atgagcaaaa ttgaagctca cctggcgcaa accgcttaag ttcttctccc
     6061 gctcgcaagg gcgggttcgc tttcccacag gagttcctca tgacgaatcg tatctctcgc
     6121 ctcaaaactg cactgttttc caatacccgt gaaatctcgc tggagcgggc gctgctttat
     6181 accgccagcc atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc gaaagcaaca
     6241 gcgtatatcc ttgaacatgt tgaaatttcg attcgtgatg aagaactgat tgccggtaac
     6301 cgcaccgtaa aaccgcgcgc cgggattatg tcgccggaaa tggaccctta ctggctgctg
     6361 aaagagctgg atcaattccc gacgcgtccg caggaccgct ttgctatcag cgaagaagat
     6421 aaacgtatct accgcgaaga gttgttcccg tactgggaaa aacgttcgat gaaagatttc
     6481 atcaacgggc agatgacaga tgaagtaaaa gccgcgacca gcacgcagat tttcagcatt
     6541 aaccagacgg ataaaggcca ggggcacatt attattgatt acccacgcct gctgaatcac
     6601 gggctggggg agctggtggc gcagatgcag caacattgtc agcaacagcc ggagaatcac
     6661 ttttatcagg ccgcgctgtt actgctggaa gcctcgcaga aacacattct gcgttacgct
     6721 gaactggcgg aaacaatggc ggcaaactgc acagatgccc agcgtcgcga agaactgctg
     6781 actattgcgg agatctcccg ccataacgcc gaacataagc cgcagacgtt ctggcaggcg
     6841 tgccagttat tctggtacat gaacatcatt ctgcaatacg aatccaacgc cagttcgctg
     6901 tctttagggc gcttcgacca gtatatgttg ccgttctatc aggcttcttt aacccagggc
     6961 gaagatccgg cgttcctgaa agaactgctc gaatctttat gggtgaaatg caacgacatc
     7021 gtgctgttgc gctccaccag cagcgcgcgt tatttcgcag gtttcccgac cggctatacc
     7081 gcactgctcg gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctt
     7141 tgccttgacg cctatcaaag cgtgcaatta ccgcaaccga acctcggcgt gcgcactaac
     7201 gccttgatcg acacgccgtt cctgatgaaa accgccgaaa ccattcgcct cggcaccggt
     7261 attccgcaaa tctttaacga tgaagtggtg gtaccagcgt tcctcaaccg tggcgtttcg
     7321 ctggaagatg cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat tcccggcaga
     7381 acctacggct tgcatgacat cgcgatgttt aatctgctga aagtgatgga aatctgcctg
     7441 catgaaaatg aaggcaatgc tgcgctgact tatgaaggtt tactggagca gatccgcgcc
     7501 aagatcagcc actacatcac cctgatggtt gagggcagca atatttgtga tatcggccat
     7561 cgcgactggg cacctgtacc gctgctctcg tcttttatca gcgattgtct ggaaaaaggt
     7621 cgcgatatta ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg gatcggtatc
     7681 gccaacctga gcgattctct ccatgcgttg aaagggatgg tttttgagca acagcgttta
     7741 agttttgacg aattgctgtc ggtattaaaa gccaactttg caacgccaga aggcgaaaaa
     7801 gtccgtgctc gcttaattaa ccgcttcgag aaatacggta acgatatcga cgaggtggat
     7861 aacatcagcg ccgaactgtt acgccactac tgcaaagaag tggaaaaata ccagaacccg
     7921 cgcggtggtt acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg
     7981 gtggttggcg cgacgccaga cggtcgtttt gccggggaac aactggcaga cggcggcttg
     8041 tcacctatgc tgggccagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag
     8101 ctcgataata cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc
     8161 ctggaaggtg aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt
     8221 aagttgcagc atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag
     8281 cgcccacaag attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc
     8341 gaactgtcga aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt
     8401 tgtggaaacg cgccgcaatg atgtggcgcg cattttcaac attcagcgtt attcactgaa
     8461 tgacggcgag ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccatg
     8521 gtgtgctaat ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg
     8581 tctgcactgt gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg
     8641 gattggtcgc gatatcagcc ttgacgcgct ggaacgggaa gtgatgaaag atgacatttt
     8701 ctttcgcacg tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga
     8761 gtttgctacc cgttttttac agcggctgcg gttgtggggc gtgtcatgcg ccattgaaac
     8821 tgccggagac gtgccagcca gcaagctgtt accactggcg aaattgtgcg atgaagtgct
     8881 gttcgattta aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc
     8941 acgcgtgctg gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt
     9001 accgttgatc cctggtttca cgctcagccg ggagaatatg cagcaggcgc tggatgtact
     9061 gatcccgctg aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa
     9121 ataccgcctg ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga
     9181 tgtgacaacg atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta
     9241 aaatggcata cctggtggca gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg
     9301 cggcggaacg gctggaaaag ttgtgccagt tagagaagtg gggcgttagc attgaaactc
     9361 agggcgcgct gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg
     9421 ctctgttgat tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg
     9481 tgcaatgcag catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca
     9541 aagtgctttc tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag
     9601 ttggctgtgg tactgccgac gatattccga cggcgagcgt tcggtgtttt tacgaaacag
     9661 acggcagaag tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag
     9721 gtcatagcct ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt
     9781 aaaaccaatg gcccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa
     9841 tgcctgtgcg acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg
     9901 aatagcttca aatagtgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc
     9961 gcggcagtgg ctaagcaggc tggcgacgat aagccttgcc gtttgctgct cctgcggctg
    10021 catttgcatt tcattgagcg tttgtagcag aaaagaacct atgcgtgggc cgcgccgggc
    10081 gacgtgttgc ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc
    10141 gagttgctgt ttgccaaaca gcacgctaaa ggtagtagcg ggggcttgcc attgtggaaa
    10201 attccagcca ccagcaggaa cgtacagcac atcgccggga actaacgttg ctccagcgcc
    10261 ggtatcaaca aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc
    10321 gagatcgggc accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc
    10381 gttgatcagg cgggagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg
    10441 tagacgggaa aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag
    10501 gggagttcga tgtaaacatt gcggcagtat cgcatccggc aaatagcgcc agacgcgata
    10561 caaaggtcat ttactgatta cccacctgat cgccatacgg cagcgtgtcg taatcgatca
    10621 gcgcgttttt cttgtacggg ttaccaatcc agcgggtagt ttctttgaac tgcggattca
    10681 ccactgaacg ggttggatcg taaccgtcgt aagataagcc tgctaaatct gaccaggtgt
    10741 ggatcagttc cgccaggctg tatttacgat caacatcctg cgagaaatca cgcggatgag
    10801 tcgcttgcca tttttccgac gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg
    10861 ggttgtcttc gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt
    10921 ggtcagagaa ataaaccagg aaaccgttcg ggtttgctgc tttaaagtct ttaatcaggc
    10981 tggcaaccac atgatcgtta tacaggttag cgttgtcata atcgttatat gactccagct
    11041 cttccgcgtt taatccaggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt
    11101 ccgggtagcg gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct
    11161 tcggcgccgg gtccttcagc acttcctgga atggtttcag cacgttggtg tcgtattcac
    11221 gcgcactctg cgtacgttgc tggttcatat agaactgttt gtcggtctgc ttcgaaaata
    11281 ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat
    11341 aacctgcctg tttcatcatg ttcatcagcg acggttgtgt caggtacagg tccgggttct
    11401 tttcattagc aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa
    11461 ccacgttatt aaacacggtc agattcggat cggttttatg cagcgcatcc agctccggcg
    11521 tggtttcacg cggataaccg tacagactca tgcgcccgcg ttgggtcgat tcgccaatca
    11581 ccagcactaa ggtacgcggt tcgttacccg attcatcttt gaaattagcc agaggcggca
    11641 aggcattatt ttcattcagt aactttgtta gcgagtttag ttgctgacga tactgataat
    11701 agccggtcag gaattgccac ggtgcggcag gctccatacg cgaggccagg ttatccaacg
    11761 ttttctcaaa cggcttgttt ttgataaacg tattcatggc gatcggatgc agaatcaagc
    11821 cataaagcag ggcaaaagag acaacataac gccacggctt tggaatatag accgggtgca
    11881 ggcgtgtcca cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca
    11941 ggctgaaata ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa
    12001 acagaacgct ctgcgagaac tcctgcccgt agatgacgta gtagcatagc gccgccagag
    12061 aggccgccca tagcaccacg ccgattactg cggcaataat tttaatccgc ttcggaaaga
    12121 ggaataccgg gatcaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc
    12181 cactataacc actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga
    12241 gcagtgccca acccagggct ttccagctaa aaaggggttt agcctggact tctgtggaat
    12301 gcatagtaag aacctgtctt gaaaaaaata tcgccgaatg taacgacaat tccttaagga
    12361 tatctgaagg tatattcaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg
    12421 gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga
    12481 ggaagattag ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc
    12541 ttgttcgacg cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag
    12601 taactcggcc tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc
    12661 tgcaatccac ggcagatcgg ccattagatg ggaatcgttg gcaatcgcca gcaccacttt
    12721 gatgtcttct tcttgcaggt tgcgtaactc tttacctaac ggccacagtg ctttgtctac
    12781 caggcgttgg tcatagtatt ccgccagcca caggtctgct ttggcgaaga ccatctccag
    12841 catgccgaga cgcgtcgaga agaatggcca atcgcggcac atggcttcca gctcgctctg
    12901 tttgccatct tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat
    12961 caggcggttt tgcgtccagg caaaaatcca cggtatggcg cgcagcgact cgacgccgcc
    13021 ggttgggcga cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt cttgttccgg
    13081 cgtagcggag cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag
    13141 atcgcaggag atgactgaca gttcatccat aatgcgacgc cagctctctt tcggctcagg
    13201 tggtggcagc aggttggctt ccagaatagc cccggtgtac agcgacaggc tgctgacggt
    13261 gatttctggc agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc
    13321 acctttcagg cttcctggcg gttgtgacag cagcgccgca tgagcaggtg cgccgccgcg
    13381 accaatggaa ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt
    13441 tttgattaat gcatcctgtg cctgatattg cgcccaggaa gctgccatca ctcccgcatc
    13501 ttttgctgag tcggaatagc caatcatcac catctgtttg ccctgaatca ggccacgata
    13561 ccagtcaata ttgagcagct gggtcatgac atcgttggcg ttgttcagat catcgagggt
    13621 ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg
    13681 gacagccagt acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc
    13741 ttgcggtgct tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg
    13801 ttgccagttg cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg
    13861 tttgtcggcc tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc
    13921 cagcgcttcg gtatgacgcg tgctctcctg acggatatca atacggacca gcggtacgcc
    13981 gaaacatttc acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc
    14041 acacgcctga agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt
    14101 cagcaggcct tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg
    14161 tgtcgccatc aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc
    14221 ttcttcgcca accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac
    14281 cagcacctga atatctttca ggaacaaatc ggtggctttc cagcggctga gtagcaggac
    14341 gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg cggtcgccgc ccatccacga
    14401 agtaaaacgg accggaacaa attcgacggg cagtttgtag ccgaggttct cttccagttg
    14461 ttcgttcagt tcgcgcaggt aatttggtac gccttgccac aggctgtttt ccactacggc
    14521 aaagccccat ttggcttcat ctaccgggct tggacgcagc ttacggattt catcggtatg
    14581 ccatgactgg gcgattaact ggcgcaggcg gcgcatcagc tggttgtgtt cgtagtcagc
    14641 gatatctttg ttatcgagct gttttaaaca ggcgttcact tccaccattt tgtggatcag
    14701 tgtacgacgg gtaatttcgg ttgggtgagc cgtgaggacc agttccagcg acagcgattc
    14761 cactgctttt ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tacgcagggt
    14821 gcgggcgatc acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg
    14881 ctcggcggtg ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc
    14941 gtcgttcgac aaattttgta aggtggtgag caactcctgg cggttagcat cattgccagc
    15001 gcgtgaagat ttcgacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa
    15061 cgcatccttg atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc
    15121 ggaatattgt tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat
    15181 ccttcacgtc gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag
    15241 ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca
    15301 ggaatttacg ccattaaatt cacgacgctt taaataagcg taacttatgg aaatgttaaa
    15361 aaatcgccat acataacacc aaaggtgtag gtcggataag atgcgcaagc atcgcatccg
    15421 acattattgc ggcacaggag tttggcaacg gtgccggatg cggcgcgagc gccttatccg
    15481 gcctacgttt taatgccagc aaaaatggtg aattacctgg gttatcagtt cgcgggtggg
    15541 cttgataaac cgtgtttcca gatattcatc aggttgatga gcctgattaa ttgagccagg
    15601 ccccaacacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtagttcac
    15661 cacttcggtc tttgctccga gcaatttctc aactacttca accagttgat gattcggtgg
    15721 gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg
    15781 ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgcc
    15841 aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt tagaagcgtc
    15901 gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg cttcgtagtg
    15961 ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag
    16021 ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg
    16081 gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc
    16141 aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat
    16201 actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc
    16261 gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa
    16321 gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc
    16381 atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc cctgtccgat
    16441 acttgccagc atattgaatt tgttgcgagt tcctggcaca ggctgcactt ccacattgaa
    16501 gcctaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag
    16561 tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa
    16621 tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat
    16681 gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag
    16741 gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg
    16801 aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctatgtaaat
    16861 cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga
    16921 aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc gttgcagccg
    16981 atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc caccgcccat
    17041 gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt
    17101 tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat
    17161 caatacccgg aactgttgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa
    17221 ttaaaagaag cgaatttgat tgcggtgccg ggctgttatc cgacggcggc acagctggcg
    17281 ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc
    17341 accagcggcg tgagcggtgc aggacgtaag gcagcgattt caaatagttt ttgtgaagtt
    17401 agcctgcaac cgtatggcgt ctttacccat cgccatcaac cagagatcgc cacacacctc
    17461 ggtgctgacg ttatcttcac cccgcatctg ggcaatttcc cgcgcggcat tcttgaaacc
    17521 attacctgcc gcctgaaacc gggtgtgacc caggcgcaag tggcgcaagc gttacagcag
    17581 gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat
    17641 gtcgttgggc tgccatgttg cgatatcggg tttgccgttc agggcgagca tctgattatt
    17701 gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat
    17761 attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt
    17821 aattatcaaa ctgggcggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag
    17881 cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg
    17941 ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaatggcct
    18001 gcgggtgacg cctgcggatc agatagacat tatcaccgga gcactggcag gaacggcaaa
    18061 taaaaccctg ctggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg
    18121 tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc
    18181 gcagccaggt tcgcctaagc ttatcaacac cttgctggag aacggctatc tgccggtggt
    18241 cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc
    18301 gacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctctgatg tcagcggcat
    18361 tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat
    18421 tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgctgc
    18481 gcgcacgttg ggccgtccgg tagatatcgc ctcctggcgt catgcggaac agcttccggc
    18541 actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt tggccggagg
    18601 cgcagctttc cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg
    18661 gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga
    18721 ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccctggtaac
    18781 ggtcggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga acgtgttgct
    18841 ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag
    18901 ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg
    18961 gcgtagccgt aatgatcagg tagcgacaga cctgaaactg tggtgcaaag ataccgttgg
    19021 cgagttgctg acggctaacc ggcagctgca atcggcgctg gtggaaaccg cacaaaacaa
    19081 tcaggacgcg gtgatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc
    19141 gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc
    19201 gcttaagcgt ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa cggcctatga
    19261 aatcgaccgt gaacagttag caggctggct gggctttgct tcggcgaccc gtaacagtct
    19321 cgacagcgtt tctgaccgtg accatgtgtt ggaactgctt tctgctgccg ctatcggcat
    19381 ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt
    19441 tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc
    19501 ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggggcgt taaccggcat
    19561 gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga
    19621 aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg cgctggtgct
    19681 ggacggcatt caggtgaaac gtccacgttg ccaggaagcg gctcagcagg gttacgccaa
    19741 cgccaccgaa ctggcggatt atctggtggc gaaaggcgta ccgttccgcg aggcgcacca
    19801 tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg aagatctgcc
    19861 gctcgacgag ttgcagaaat tcagcccggt gattgacgaa gatgtctatc cgattctgtc
    19921 gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc
    19981 gcaggcaatt gcttttgcgc aggcgcggtt ggggtaagaa catttatatg tataaatttg
    20041 agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg agatccgcaa
    20101 aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc agtgacttca
    20161 agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa tgcttgatag
    20221 ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcaa tggaggatgg
    20281 ataatgaata ttcgtgatct tgagtacctg gtggcattgg ccgaacaccg ccattttcgg
    20341 cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat tcgtaagctg
    20401 gaagatgagt tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt gttcacccag
    20461 gcgggaatgc tgctggtaga gcaggcgcgt accgtactgc gtgaggtgaa agtccttaaa
    20521 gagatggcaa gccagcaggg cgagacgatg tccgggccgc tgcacattgg tttgattccc
    20581 acagttggac cgtacctgct accgcatatt atccctatgc tgcaccagac ctttccaaag
    20641 ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact ggacagcggc
    20701 aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat tgaagtgccg
    20761 ttgtttgatg agccaatgtt gctggctatc tatgaagatc acccgtgggc gaaccgcgaa
    20821 tgcgtaccga tggccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt
    20881 ttgcgcgatc aggcaatggg tttctgtttt gaagccgggg cggatgaaga tacacacttc
    20941 cgcgcgacca gcctggagac gctgcgcaac atggtggcgg caggtagcgg gatcacttta
    21001 ctgcctgcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta tctgccgtgc
    21061 attaagccgg aaccacgccg cactattggc ctggtttatc gtcctggctc accgctgcgc
    21121 agccgctatg agcagctggc agaggccatc cgcgcacgaa tggatggcca tttcgataaa
    21181 gttttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt ccgccatcgt
    21241 cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac ctttctgttc
    21301 cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc
    21361 cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt tcatgccgac
    21421 gatttgtgcg cgtgccagat gtttaaactg ggcgcggccc acttcatatg gcactttcat
    21481 tgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg tgtaaatgcc
    21541 ggtagggata tcttcaatca gatgtgcggt ggcttcgcct tttaccagcg cctgcgcggc
    21601 aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca cgtcgcccac
    21661 cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct gtccgcggct
    21721 gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg tgcgaccgtt
    21781 ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcaaatgca tgatcacacc
    21841 atcgtcacag ccttcgatct tctcgtactc ttcgttgtga cgaatcacta cgccactgtt
    21901 ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca gcaggcgatc
    21961 gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg catattcaca
    22021 gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca tgctaagaat
    22081 tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat gatatggacg
    22141 agagccgcag gcaataacaa atttttcagc ggttagtgtt tcaacgctgc cgtccgggca
    22201 atccagcgcc aacgtatgct cgtcaacaaa gcgagcgttt ccctgcaata tttcacagtg
    22261 attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg
    22321 gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt aaagtgggtt
    22381 ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg ggatggtgcc
    22441 ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga cgcgcgcacc
    22501 ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta ttactatggc
    22561 atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat aaaagcaaca
    22621 gaatggtaac attttatcgc gggtaagcca attgatcccc gtcatttatc tggctatatc
    22681 ctgagcggcc tttgctttgt ctgtttctta cttttgccct gacgttttat tggattttta
    22741 tcgacgatac tctccgttta agcggcaggt ttccgctgta cgtaaaagaa ccggccaaag
    22801 aattgcagta aatatgtttt attgcgttac cgttcattca caatactgga gcaatccagt
    22861 atgttcattc tctggtatag tgccagcagt acttttggca aggattcaga catcgtgatg
    22921 ggcgtaagag cgcaacaaaa agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc
    22981 caattaagtg ctgaacgcag cttcaccagc ctgagtttgc gtgaagtggc gcgtgaagcg
    23041 ggcattgctc ccacctcttt ttatcggcat ttccgcgacg tagacgaact gggtctgacc
    23101 atggttgatg agagcggttt aatgctacgc caactcatgc gccaggcgcg tcagcgtatc
    23161 gccaaaggcg ggagtgtgat ccgcacctcg gtctccacat ttatggagtt catcggtaat
    23221 aatcctaacg ccttccggtt attattgcgg gaacgctccg gcacctccgc tgcgtttcgt
    23281 gccgccgttg cgcgtgaaat tcagcacttc attgcggaac ttgcggacta tctggaactc
    23341 gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg aagcaatggt gacaattgtc
    23401 ttcagtgcgg gtgccgaggc gttggacgtc ggcgtcgaac aacgtcggca attagaagag
    23461 cgactggtac tgcaactgcg aatgatttcg aaaggggctt attactggta tcgccgtgaa
    23521 caagagaaaa ccgcaattat tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc
    23581 aagatagagg tacgctgctg ctggcgttag ttgctggctt atcgattaat ggtactttcg
    23641 cagcgctgtt tagctccatt gtgccatttt ctgtattccc gattatttcc ctggtgctga
    23701 cggtttactg cctgcatcaa cgttatctta atcgcactat gccggtaggc ttgccgggtc
    23761 tggcagctgc ctgttttatt ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat
    23821 atccggatat cggctctaac ttcttcccgg cagtactctc cgtcattatg gtgttctgga
    23881 ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga ataatcggta ttatgccgga
    23941 tgctggcgca tccagcatgg gttttacttc gcggtcagta atacgccgca ctccatatgg
    24001 tgcgtgtagg ggaactgatc aaacagagcc agacgttcga ccttgtgcgt ctggcttaat
    24061 gtttccagat tcttgcataa cgtttccggg ttacaggaga tgtacaaaat acacggatac
    24121 gcctgcacca ttttctcggt ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg
    24181 gtttcgcact gataactctt taagtcgatc ccttgcaggc ggttaaactc gcgcacacca
    24241 ttcatcgcct gagtaaattc ttctgccgcc atacgaataa tttgtacgtt atcaatatgg
    24301 ttagctgcga tgttgtattg cgcagcagca accgacggct tagcgatttc ggtggctaat
    24361 acccgatcaa aattgcgcgc cagcgctaat gaaaagttac cgttgccgca gtacagctcc
    24421 agtaaatcgc cttttgagcc tttggttacg tccagcgccc attccagcat ctgaatattc
    24481 atcgccgcgt tcggctgggt aaagctgttt tctacctgac ggtagatcat ctctttccct
    24541 gcgaccggca gacgctcgtc gatgtaatct tgatccagct cgattttggt tttcgttgcc
    24601 cgaccaatca gatgcacatt cagattctgc gcgcgcagtg catcgcgcag ggcctccgct
    24661 tcctgacgcc actcatcatc cagcttctta tggtatagca gggaaaccac cgcctgatta
    24721 ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc
    24781 acacccgcaa tcatcgccgt catcaactgg ttgataagtt cactggcggc ggggaagcta
    24841 tccacgcgga tgcggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca
    24901 tcgtgccaga tgcggaactc cgcgcgcatc cggtaatgac tgaccggcga gcgaaacact
    24961 tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct
    25021 aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa
    25081 ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct
    25141 atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgccggtcc
    25201 tgtgagttaa tagggaatcc agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt
    25261 gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct
    25321 tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg
    25381 cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa
    25441 aaaagcttcg ctgctgacgg cgtgttctgt cacagccttt tccgcttggg cacaggatac
    25501 cagcccggat actctcgtcg ttactgctaa ccgttttgaa cagccgcgca gcactgtgct
    25561 tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa
    25621 tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct
    25681 ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ttggtgttaa ttgatggcgt
    25741 acgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc ctattgcgct
    25801 tgtccagcgt gttgaatata tccgtgggcc acgctccgcc gtttatggtt ccgatgcaat
    25861 aggcggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg
    25921 gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac tgggggataa
    25981 gacacgggta acgctgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta
    26041 tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta
    26101 tggcgcgctg gagcataact ttactgatgc ctggagcggc tttgtgcgcg gctatggcta
    26161 tgataaccgt accaattatg acgcgtatta ttctcccggt tcaccgttgc tcgatacccg
    26221 taaactctat agccaaagtt gggacgccgg gctgcgctat aacggcgaac tgattaaatc
    26281 acaactcatt accagctata gccatagcaa agattacaac tacgatcccc attatggtcg
    26341 ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt gggcaaacaa
    26401 tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac agactacgac
    26461 gccgggtaca ggttatgttg agaatggata tgatcaacgt aataccggca tctatctgac
    26521 cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagtg acgataactc
    26581 acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca tcgaaggtta
    26641 tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc aactgtatgg
    26701 cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga
    26761 aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgactt
    26821 gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg
    26881 cgtcgaggcg accgccaatt ttgataccgg accactgacg catactgtga gttatgatta
    26941 tgtcgatgcg cgcaatgcga ttaccgacac gccgttgtta cgccgtgcta aacagcaggt
    27001 gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt atcagtattt
    27061 aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg
    27121 tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcatctga cagttcgtgg
    27181 taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg
    27241 acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt
    27301 ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc
    27361 tccattacat ttatgctttc gataacgtcg cttttccgta tggcgaaaaa agcgaagagt
    27421 ttattgttga gcgagtggtg gcaattgtca ccgcagtgca agaacgttat ccccttgcgc
    27481 tggctgtggt cgcttgcaac accgccagta ccgtttcact tcctgcatta cgcgaaaagt
    27541 tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa
    27601 atggcattgt cggattactg gcaacccgcg gaactgttaa acgttcttat actcatgagc
    27661 tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg
    27721 agttggctga agcgaagctt catggcgaag atgtttctct ggatgcacta aaacgtatcc
    27781 tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt tgcacccatt
    27841 tccctctact acaagaagaa ctcttacagg tgctgccaga gggaacccgg ctggtggatt
    27901 ctggcgcagc gattgctcgc agaacggcct ggctgttaga acatgaagct ccggatgcaa
    27961 aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat
    28021 tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat
    28081 ttggttgaat gttgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata
    28141 actccctata atgcgccacc actgacacgg aacaacggca aacacgtcgc cgggtcagcg
    28201 gggttctcct gagaatctca acagagaaaa gcaaaaaatg cttgactctg tagcgggaaa
    28261 gcgtatt
//
LOCUS       DAZFKM010000060        27863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFKM010000060 DAZFKM010000000
VERSION     DAZFKM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..275
                     /locus_tag="ACHKAY_002593"
     CDS             6..275
                     /locus_tag="ACHKAY_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5701256.1"
                     /translation="MNILKKLMQRMCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRGDGTWENIGGMK"
     gene            complement(392..667)
                     /locus_tag="ACHKAY_002594"
     CDS             complement(392..667)
                     /locus_tag="ACHKAY_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO5701257.1"
                     /translation="MPFSIKNIFSNSKGSYPEISGPVQDKPVSKNCTLTSTTCSMNDY
                     TVFSRKSCTFDMRPPGAGDRTPQLKLSASELIWLSKTIDTERNNIKE"
     gene            complement(730..2091)
                     /gene="espK"
                     /locus_tag="ACHKAY_002595"
     CDS             complement(730..2091)
                     /gene="espK"
                     /locus_tag="ACHKAY_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO5701258.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSAGIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTILTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIIPEGIECLTVCRCHISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLQTLSLTGCSNIILPEILPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKSH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADEIVNSLKLIANKSDG
                     YGQSITASELRWIYRNKDNDQIMKNIKFYLRGKEIPAEKILGAPEWKDYKPKYSGATY
                     KYS"
     gene            2480..2620
                     /locus_tag="ACHKAY_002596"
                     /pseudo
     CDS             2480..2620
                     /locus_tag="ACHKAY_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(2702..2789)
                     /locus_tag="ACHKAY_002597"
     tRNA            complement(2702..2789)
                     /locus_tag="ACHKAY_002597"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2753..2755),aa:Ser,seq:tga)
     gene            3216..4334
                     /gene="hyaA"
                     /locus_tag="ACHKAY_002598"
     CDS             3216..4334
                     /gene="hyaA"
                     /locus_tag="ACHKAY_002598"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO5701259.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            4331..6124
                     /gene="hyaB"
                     /locus_tag="ACHKAY_002599"
     CDS             4331..6124
                     /gene="hyaB"
                     /locus_tag="ACHKAY_002599"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO5701260.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            6143..6850
                     /gene="hyaC"
                     /locus_tag="ACHKAY_002600"
     CDS             6143..6850
                     /gene="hyaC"
                     /locus_tag="ACHKAY_002600"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO5701261.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            6847..7434
                     /gene="hyaD"
                     /locus_tag="ACHKAY_002601"
     CDS             6847..7434
                     /gene="hyaD"
                     /locus_tag="ACHKAY_002601"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO5701262.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            7431..7829
                     /gene="hyaE"
                     /locus_tag="ACHKAY_002602"
     CDS             7431..7829
                     /gene="hyaE"
                     /locus_tag="ACHKAY_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO5701263.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            7826..8683
                     /gene="hyaF"
                     /locus_tag="ACHKAY_002603"
     CDS             7826..8683
                     /gene="hyaF"
                     /locus_tag="ACHKAY_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO5701264.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            8817..10362
                     /gene="appC"
                     /locus_tag="ACHKAY_002604"
                     /pseudo
     CDS             8817..10362
                     /gene="appC"
                     /locus_tag="ACHKAY_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            10374..11510
                     /gene="appB"
                     /locus_tag="ACHKAY_002605"
     CDS             10374..11510
                     /gene="appB"
                     /locus_tag="ACHKAY_002605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5701265.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            11523..11615
                     /gene="cbdX"
                     /locus_tag="ACHKAY_002606"
     CDS             11523..11615
                     /gene="cbdX"
                     /locus_tag="ACHKAY_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO5701266.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            11695..12993
                     /gene="appA"
                     /locus_tag="ACHKAY_002607"
     CDS             11695..12993
                     /gene="appA"
                     /locus_tag="ACHKAY_002607"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO5701267.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(13108..15288)
                     /gene="etk"
                     /locus_tag="ACHKAY_002608"
     CDS             complement(13108..15288)
                     /gene="etk"
                     /locus_tag="ACHKAY_002608"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO5701268.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNIYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(15308..15754)
                     /gene="etp"
                     /locus_tag="ACHKAY_002609"
                     /gene_synonym="yccY"
     CDS             complement(15308..15754)
                     /gene="etp"
                     /locus_tag="ACHKAY_002609"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO5701269.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(15742..16881)
                     /gene="gfcE"
                     /locus_tag="ACHKAY_002610"
     CDS             complement(15742..16881)
                     /gene="gfcE"
                     /locus_tag="ACHKAY_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO5701270.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(16927..19023)
                     /gene="gfcD"
                     /locus_tag="ACHKAY_002611"
     CDS             complement(16927..19023)
                     /gene="gfcD"
                     /locus_tag="ACHKAY_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5701271.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(19023..19769)
                     /gene="gfcC"
                     /locus_tag="ACHKAY_002612"
     CDS             complement(19023..19769)
                     /gene="gfcC"
                     /locus_tag="ACHKAY_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5701272.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(19766..20410)
                     /gene="gfcB"
                     /locus_tag="ACHKAY_002613"
     CDS             complement(19766..20410)
                     /gene="gfcB"
                     /locus_tag="ACHKAY_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO5701273.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(20517..20822)
                     /gene="gfcA"
                     /locus_tag="ACHKAY_002614"
     CDS             complement(20517..20822)
                     /gene="gfcA"
                     /locus_tag="ACHKAY_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO5701274.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(21264..21476)
                     /gene="cspH"
                     /locus_tag="ACHKAY_002615"
     CDS             complement(21264..21476)
                     /gene="cspH"
                     /locus_tag="ACHKAY_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO5701275.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            21762..21974
                     /gene="cspG"
                     /locus_tag="ACHKAY_002616"
     CDS             21762..21974
                     /gene="cspG"
                     /locus_tag="ACHKAY_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO5701276.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            21985..22173
                     /gene="ymcF"
                     /locus_tag="ACHKAY_002617"
     CDS             21985..22173
                     /gene="ymcF"
                     /locus_tag="ACHKAY_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5701277.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            22148..22378
                     /gene="ymcE"
                     /locus_tag="ACHKAY_002618"
     CDS             22148..22378
                     /gene="ymcE"
                     /locus_tag="ACHKAY_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO5701278.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            22368..22541
                     /gene="gnsA"
                     /locus_tag="ACHKAY_002619"
     CDS             22368..22541
                     /gene="gnsA"
                     /locus_tag="ACHKAY_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5701279.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(22589..23662)
                     /gene="yccM"
                     /locus_tag="ACHKAY_002620"
     CDS             complement(22589..23662)
                     /gene="yccM"
                     /locus_tag="ACHKAY_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5701280.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(23734..26478)
                     /gene="torS"
                     /locus_tag="ACHKAY_002621"
     CDS             complement(23734..26478)
                     /gene="torS"
                     /locus_tag="ACHKAY_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO5701281.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            complement(26573..27646)
                     /locus_tag="ACHKAY_002622"
     CDS             complement(26573..27646)
                     /locus_tag="ACHKAY_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701282.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSDSGRKSLIDRIKGGDAITLHVWLDRYERILTERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIASKH
                     HDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDLMAARYRDSRGREWDKIEIDK"
     gene            complement(27624..27842)
                     /locus_tag="ACHKAY_002623"
     CDS             complement(27624..27842)
                     /locus_tag="ACHKAY_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5701283.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         6559 a         6983 c         7112 g         7209 t
ORIGIN      
        1 atgacatgaa catactaaaa aaacttatgc agcgtatgtg tggttgcgga aagcatgatg
       61 gccgtgaaca cgtgcagtcg cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtccgatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgcg tggtaagacc gctgcaaatt ctgcgtggtg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaactcga cagcttcaca aaaccggagt
      301 ccggctccat tttaccacct ccacgggagg tggttgcttg caatcagatg tttcgggcct
      361 gcggcttttt gtgttcccta gtaagtggtt actattcttt tatgttattc ctctctgtat
      421 ctattgtctt agatagccat atgagttcag atgctgagag ttttaattgt ggtgttctgt
      481 ctcctgctcc gggtggacgc atgtcaaaag tgcaggattt tctgctgaat actgtgtagt
      541 cattcataga gcatgttgtt gaggttagtg tacaattctt cgatactggc ttgtcctgca
      601 caggtccgga tatttctggg tagcttcctt ttgaatttga aaatatgttt ttgattgaaa
      661 atggcatcgt gctttgtcct ttgaactacg atatattgtt ttgtgttctt tcgttgtgag
      721 taggtatcat taagaatact tatatgtggc tccagagtat ttgggtttgt agtctttcca
      781 ctctggtgcg cctaatattt tttctgctgg tatctctttc ccacgcaagt aaaactttat
      841 gtttttcatt atctggtcgt tgtctttgtt gcgataaatc catcgtaatt cgctggcagt
      901 aatactctga ccataaccat ctgacttatt agcaatgagt ttgagtgagt tgacaatctc
      961 gtcagcacaa aaaataaccg ttttgttgcg tgttaatgtc tgaaactcca ggccggcctt
     1021 gcttgttttt gcccataatt tgtcgcgatt gtatacggaa aggtcggcca tggtgacatt
     1081 ataattattg tggctcttta ggtgttgttt aaattttatc cctctttcgt gttcatttaa
     1141 tgctcttttg atcagtggat cttgcttata tttgcgaggt gttctggggt cccatactgc
     1201 gtttgttaac gtattctgag ttataatatc ttttttggaa aagtcgttaa ctagtttaat
     1261 gctgttgaca agtttttctc tgggtgaaga tagtccatag acaatgtctc cctcgcgaaa
     1321 gtgtaaggcc acatccagag cgttgccctg aaaggtgatg ttttttgctt taactacatc
     1381 tggagagagt agtacatttt gtaaatcaag atccagccca tcaggcatcc cttcaacacc
     1441 gatgttccac gtggttttct gctccgcatg aatggttacc gatgtcacac tctccggaag
     1501 tatctccggc agtataatat tgctacatcc agtcagcgat agcgtctgca gtgacggtga
     1561 tatcaggttg tcaattctgg cctgattctc cgggttatag ctattgatgc tgagagatga
     1621 taacccgttt ggaacatttt ttatgctgtc tgtggcgcta ccttttatct caaggtagcg
     1681 gacgctctct ggcactccag atatatggca gcgacaaacc gtcaggcatt cgatgccttc
     1741 aggtatgatg tgtataaatg gtgaatcagt taattcaata tgtagatttt ttactgtcga
     1801 tggtatgtct gtgatttgta atttttcaca gttaatgacg gagaggtttt gtgtattaac
     1861 cggcaatcct gtaaggattg tcaggttgtt gcagttttcg attgtaactg atgttatttc
     1921 gtttggtaac tgtggcagat actcaatatc acaatctttg atatataaac gaccactggc
     1981 ccgcgcttct tctatctgaa ttggtgtgat ttcgcttttg atgcctgctg atgtttcagc
     2041 ggaataggag cagaatgtcc cggtcggtcg taattgcgat gtaggaagca ttgataaatt
     2101 ccatttttag aagtaaataa cagagtaagt tctatccata ctactaatca acaagctaat
     2161 tttaacaacc agcaaagcca aggtgtcaat attagaaaag agagttatat ctcaaaatca
     2221 acagactgtt gattttaact tttgtgaata ctttctccag tccgtcatgc atggattagg
     2281 attgctcatt tttataccat ttgttgtttt ttatagcgat ttgctaaaag ggagaagaaa
     2341 aaacgttctc caaaattaga ttgcaactgt ttgattttat ggatagaggc atgctgttta
     2401 ctagacagta aaaagcatgc caaatttgct attaaccttt tgaatataaa gcactaatta
     2461 tctatttctg ttgccttctt cagtagatct ggagatcgca acggaggaag aaaactcgtt
     2521 gctggaagcc tggaagaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc
     2581 tgatattgag tggccggaag aaccaagatc atccgaataa aaatatacca attggtatac
     2641 caagtttata ccaagagcca gaaaagcaaa ggggctacca tttggtaacc ccttgattat
     2701 ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc
     2761 ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac atcccgatgc
     2821 ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc
     2881 gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt tactcgttta
     2941 gcaaccggct aaacatcccc accgcccggc caaaagaaaa ataggtccat ttttatcgct
     3001 aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa
     3061 caatactttc tggcgacgtg tgccagtgca gaaggatgag ctttcgtttt cagcatctca
     3121 cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa gcgcccggtg
     3181 tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa acattttacc
     3241 aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt agtctggctg
     3301 ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg ctggagaaca
     3361 aaccccgcat tccggtggta tggatccacg gtctggaatg cacctgctgt accgaatctt
     3421 ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt tccctcgatt
     3481 atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttc gaagacatca
     3541 tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg ctgggcgagc
     3601 aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa cgtgccgctg
     3661 ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc gtgcaggccg
     3721 cgcggcccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac aaacccatta
     3781 tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt acttacatgg
     3841 tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgctgatg ttctatggtc
     3901 agcgaatcca cgataaatgc tatcgccgcg cccacttcga cgccggagag ttcgtccaga
     3961 gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc
     4021 ctaccaccta taacgcctgt tcttccacac gctggaatga tggcgtttct ttcccaatcc
     4081 agtctggtca cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc ggttcgttct
     4141 acagccgcgt ggtcgatatt ccgcaaatgg gtactcattc caccgccgat accgtcggtt
     4201 taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg
     4261 accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca ggcaatgagg
     4321 ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata atgccggacg
     4381 ccgcctggtg gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg aagtgaatat
     4441 taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc gcgggctgga
     4501 gatcatccta caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg
     4561 cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg ctatcggtat
     4621 taaagtgccg gacaacgcaa atatcatccg caacattatg ctggcaacgc tctggtgcca
     4681 cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg tgttagatgc
     4741 gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct cttggccgaa
     4801 atcatcccct ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg ttgaaggcgg
     4861 gcagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac tgccgccaga
     4921 agctaacctg atgggctttg cccactatct cgaagctctc gatttccagc gtgaaattgt
     4981 caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg tcggcgggat
     5041 gccttgcgcc atcaacattg acgaaagcgg cgcggtcggg gcagtcaata tggaacgcct
     5101 gaacctggtg cagtcaatta tcacccgcac ggcggacttc attaacaacg tgatgatccc
     5161 cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca ctggtctttc
     5221 tgataaatgc gttctcagct acggcgcatt cccggatatt gccaacgact ttggcgagaa
     5281 aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg tgctgccagt
     5341 ggatttggtt gatccgcagc aggtgcagga gtttgtcgac cacgcctggt atcgatatcc
     5401 caacgatcag gtcgggcgtc atccgttcga tggcatcacc gacccgtggt acaaccccgg
     5461 cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac gctactcgtg
     5521 gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg cgcgcacgtt
     5581 aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca tgatgtcggc
     5641 gttgaacctg ccgctttccg gtatccagtc aacgttaggc cgcattttgt gccgcgcgca
     5701 cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gacaagctga tgaccaacct
     5761 gaaaaacggc aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa cctggccgac
     5821 agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc actgggccgc
     5881 cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct ggaacgccag
     5941 cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga acaccaaaat
     6001 ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg acccgtgcct
     6061 cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctctg tgcaggtgcg
     6121 ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca gccactatgt
     6181 ctttgaagcg ccagtccgca tctggcactg gttgacggtg ttatgcatgg cggtgttgat
     6241 ggtcaccggg tactttatcg gcaagccgct accttccgtc agcggcgagg cgacgtatct
     6301 gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt ttaccgtggt
     6361 tttgctgatg cggatctact gggcttttgt tggcaatcga tactcccgcg agctgtttat
     6421 cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc gctggtatct
     6481 gtttctggca aaacgtccga gtgccgatat aggccataat cccatcgccc aggcggcgat
     6541 gttcggctat ttcctgatgt cggtctttat gatcatcact ggttttgcgc tgtatagcga
     6601 acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt tctactggat
     6661 gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc tgattggcgc
     6721 gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg acgacacggt
     6781 gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa gtaacaagga
     6841 gcgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct gtgggccgat
     6901 gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg gcccgagtat
     6961 gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt cgaaagcgcc
     7021 agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaac gctgcgaacc
     7081 tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct gcatcagaac
     7141 agtttctccg aagtgttggc gctggcggat atccgcggac atctgccagc acatattgcc
     7201 ctcgtcggtc tgcaacccgc aatgctcgac gactacggcg gtagcctgag cgaactggca
     7261 cgggagcaac tgcccgctgc ggaacaggcg gcgctggcgc agctggctgc gtggggaatt
     7321 gtgccgcaac cggctaatga atcgcgctgt ctcaattatg actgtctgtc gatggaaaat
     7381 tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg atgagcaacg
     7441 acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg ccagtcagtg
     7501 aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtgctgtta ttaagcagtg
     7561 acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa ttactgcgcg
     7621 agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc gaagccatcg
     7681 gcgatcgctt tggcgtcttt cgctttcctg ccactttagt gtttaccggc ggaaactatc
     7741 gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg cgcgggcttg
     7801 tcgaaccgca gcaggagcgt gcctcatgag cgaaacattt ttccatctgc tggggccagg
     7861 aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct gtcaggtgaa
     7921 tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg tgattgcgct
     7981 gttaaacgag ttacaacatc aactaagcga acgccaaccg ccgttgggcg aggtgctggc
     8041 agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc ttctcgggga
     8101 aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt gaaagtgaaa tacaggaggc
     8161 gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc tggaggacaa
     8221 actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa atctcttgcc
     8281 gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac cgttggcgca
     8341 tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta atctgacgca
     8401 attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc cgggaaatat
     8461 tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt tacgccatgt
     8521 ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt atgaaatctg
     8581 cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg cgcagcggct
     8641 tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg gtaagtatcg
     8701 ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt ttcaggataa
     8761 agagggagat ctaccattat cgggttattt ttctctcttc gcctacagga gtgcgcatgt
     8821 gggatgtcat tgatttatcg cgctggcagt ttgctctgac ctgcgctgta tcacttttta
     8881 tttgtacccc ttaccctggg gctgattttt ttgctggcta tcatggaaac catttacgtg
     8941 gtcaccggca aaacaatcta ccgcgatatg acgcgcttct ggggtaagct cttcggtatc
     9001 aattttgctc ttggcgtggc taccggcctg accatggagt ttcagtttgg tactaactgg
     9061 tcattctatt ccaactatgt gggcgatatt tttggcgcac cactggcgat ggaagcatta
     9121 atggccttct tcctcgaatc cacctttgtc gggctgttct tcttcggctg gcaacggctg
     9181 aataaatacc agcacctgct ggtgacgtgg ctggtggcgt tcggttcaaa tctctctgcg
     9241 ttgtggatat tgaatgccaa cggttggatg caatacccga ccggtgcgca ttttgatatc
     9301 gacaccctgc gtatggagat gaccagcttc agcgagctgg tctttaaccc ggtcagccag
     9361 gtgaaatttg tgcacaccgt aatggcgggc tacgtgaccg gggccatgtt tattatggcg
     9421 atcagcgcct ggtatttact gcgcggacgg gagcgcgatg tcgcattacg ttcgtttgcc
     9481 atcggttccg tcttcggtac tctggcgatt atcggtaccc tgcaactcgg agacagttct
     9541 gcgtatgaag tcgcgcaagt acaaccggta aaactggcgg cgatggaagg ggagtggcaa
     9601 acggaacctg cacctgcacc gttccatgtg gttgcctggc cggaacagga tcaagagcgt
     9661 aacgcctttg ccatcaaaat tcccgcgctg ctagggatcc tcgccactca ctcattagat
     9721 aaacccgtgc cgggtctgaa gaatttgatg gctgaaacct acccacgctt gcaacgcgga
     9781 cgtatggcct ggctgttaat gcaggaaata tcgcaaggca atcgtgagcc gcatgtgttg
     9841 caggcattcc gggaactgga aggtgacctg ggctacggca tgttgctctc ccgctatgcg
     9901 ccggatatga atcatgtcac agccgcacag taccaggcgg cgatgcgtgg cgcgatacct
     9961 caggttgcgc cggtattctg gagtttccgc atcatggtgg gctgtggttc cctgctgcta
    10021 ctggtgatgc tgattgcgct tgtccagacg ctgcgtggca aaatcgacca gcatcgctgg
    10081 gtgctgaaaa tggcgctctg gagtttgccg ttgccgtgga ttgcgattga agccgggtgg
    10141 tttatgaccg agtttggtcg tcagccgtgg gcgatacagg acatcttacc gacatactcc
    10201 gcgcactccg ctttaaccac aggacaactg gctttctcac tgatcatgat cgtagggctt
    10261 tacaccctgt tcttaatcgc cgaagtctac ctgatgcaga aatatgcccg tctggggccg
    10321 agcgcgatgc agagtgaaca accgacgcag caacaggggt aaaggagaaa atcatgtttg
    10381 attatgaaac attgcgcttc atctggtggc tgctgattgg cgtgatcctg gtggtcttta
    10441 tgatctccga cggatttgac atggggatcg gctgtctgct gccgctggtg gcgcgtaatg
    10501 atgatgaacg ccggatagtg ataaacagcg ttggtgcaca ctgggaaggc aaccaggtct
    10561 ggttgatcct cgctggtggg gcattatttg ccgcctggcc cagagtgtat gcagcggcgt
    10621 tttccggctt ttatgtggcg atgatcctgg tgctgtgctc actgttcttc cgcccgctgg
    10681 cctttgatta tcgcggaaaa atcgccgatg cacgctggcg taaaatgtgg gacgccggtc
    10741 tggtcatcgg cagtctggtg ccgccggtag tcttcggtat cgccttcggc aacttgttgc
    10801 tcggcgtgcc gtttgccttc acaccgcaat tacgcgtgga gtatctcggc agcttctggc
    10861 aactgctgac gccattccct ttattgtgcg gattgctcag ccttgggatg gtgattttgc
    10921 aaggtggcgt ctggttacaa ctgaaaactg ttggtgtgat tcatctgcgt tcacagctgg
    10981 ccaccaaacg cgctgcactg ttggtgatgc tgtgcttttt gctggcgggt tactggctgt
    11041 gggtcggtat tgatggcttt gtactgctcg cccaggatgc taacggtcct tccaatccgt
    11101 taatgaaact ggtggcagtg ctacctagtg cctggatgaa taatttcgtc gaatcgcccg
    11161 ttttgtggat cttcccgctg ctggggttct tctgcccatt gctgacggtg atggcgattt
    11221 atcgtggtcg cccgggttgg ggatttttaa tggcgtcatt gatgcaattt ggcgtgattt
    11281 tcacggcagg catcacgctg ttcccctttg tcatgccgtc aagcgtgagt ccaatctcca
    11341 gcctgacgtt gtgggacagc acttccagtc agctgacgct gagcattatg ttggtaatcg
    11401 tgctgatatt tttgcccatt gtgttgctct acactctctg gagctactac aaaatgtggg
    11461 gacgcatgac aacagaaact ctccgccgta acgaaaacga gttgtactaa ggagcaggaa
    11521 caatgtggta tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg
    11581 tgttggtatg gctggacccg cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat
    11641 tagcatcgca tcaggcaatc aataatgtca gatatgaaaa gcggaaacat atcgatgaaa
    11701 gcgatcttaa tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct
    11761 cagagtgagc cggagctgaa gctggaaagt gtggtgattg tcagtcgtca tggtgtgcgt
    11821 gctccaacca aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacctgg
    11881 ccggtaaaac tgggttggct gacaccgcgc ggtggtgagc taatcgccta tctcggacat
    11941 taccaacgcc agcgtctggt agccgacgga ttgctggcga aaaagggctg cccgcagtct
    12001 ggtcaggtcg cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc
    12061 gccgccgggc tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt
    12121 cccgatccgt tatttaatcc tctaaaaact ggcgtttgcc aactggataa tgcgaacgtg
    12181 actgacgcga tcctcagcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa
    12241 acggcgtttc gcgaactgga acgggtgctt aattttccgc aatcaaactt gtgccttaaa
    12301 cgtgagaaac aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg
    12361 agcgccgaca atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata
    12421 tttctcctgc aacaagcaca gggaatgccg gagccggggt ggggaaggat caccgattca
    12481 caccagtgga acaccttgct aagtttgcat aacgcgcaat tttatttgct acaacgcacg
    12541 ccagaggttg cccgcagccg cgccaccccg ttattagatt tgatcaagac agcgttgacg
    12601 ccccatccac cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatc
    12661 gccggacacg atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt
    12721 cccggtcagc cggataacac gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg
    12781 ctaagcgata acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg
    12841 cgtgataaaa cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca
    12901 ggatgtgaag agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg
    12961 aatgaagcac gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttacct
    13021 gaatgctctg aggctgatga caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt
    13081 tcacgccgca tccggccact ttcagtttta ctctttctcg gagtaactat aaccgtaata
    13141 gttatagccg taactgtaag cggtgctggc gcgtttaatc acaccattga ggatagcgcc
    13201 tttaatattg acgcctgcct gttccagacg ctgcattgac aaactcacct ctttggcggt
    13261 gttcaagcca aaacgcgcaa ccagcaggct ggtgccaaca gaacgcccca cgaccgcggc
    13321 atcactcacc gccagcatcg gcggcgtatc gacaatcacc agatcgtaat ggtcgttcgc
    13381 ccattccagt aattgacgca tccgatcgcg catcagcagt tcagacgggt taggtggcac
    13441 ctgaccgcga gtaatcacat caaagcctcc tttgccaaaa tgctggatca ctttgttgag
    13501 ctcatcttta cctgccagat attccgacaa gccatgttca ttactcacgg taaacaggtt
    13561 atgcgaataa ccacggcgta agtcggcatc aataaataac actttttgat cggactgggc
    13621 gatcaccgct gccagagttg aactgacaaa cgttttacca ctgtctggcg tcgcaccggt
    13681 gatcatcaga atgttattct ccgtctccat catagcgaaa tgcagactgg ttcgtagcgc
    13741 acgtacggct tccacagcag aatccgccgg gttatccacc gccaggaagg ggatattttt
    13801 agtacgatgg cgctgctgat tagaaaataa atttttctta cgcagacggg tgcgtttatc
    13861 cagccactcg gacattggga tagtggcata aacgctgatg ccgtgctctt ccagttgttc
    13921 cggggcttct acaccacgac gcaacatcgc acgcgccagc acggcaccca cagaaataaa
    13981 caggccaaga ataaaaccaa gcaccacatt caacgctttt ttcggtttca ctggctgcgg
    14041 ctgagtgact gccgggtcga taatccgcac gttaccaatg gcactggatt tcgaaatact
    14101 caactcctgc tggcggttaa gtaattgcag atataccgca cggcccgctt ctacgtcacg
    14161 acttaaacgc aacacttcct gttgggtgga aggcattgcc gatacccgct tattcaggcg
    14221 tttgcgttct tgctccagcg tctggcgttt ttccagcagc gcacgataag ttgggtgatc
    14281 tttcttatac agctgggaga tctctgcctc gcggaaggtc agctcattga gttgattatc
    14341 aacgttcaca atctgctcaa gaacggcttt ggcttccagg ttaaggtcaa ccgaatcgcg
    14401 ctgctggcga taaatgttga gtttttcttc cgcctggtcc agctcgctgc gcacttcagg
    14461 taactggcgc tgtaagaatt caaggctttg tgaatcctgc gccgcctggc gagcgatatt
    14521 ctgttgcaaa tagttgttag cgatgctgtt cagaatacga gtaatcaact ggggatcatc
    14581 accagtcatg gtaagttcca gcatcccgct ttctttactg cgttcgctaa cggtaaaggt
    14641 ttcctgcaat gcgttaatcg cttccagttc ggtacgctgg ctcaggacaa actgtgttcc
    14701 tggtttggcc ttaatgtccg cgatagtcag cgcaacgcca tctttttcca gacgctggcc
    14761 gaccatacca ttgacggtga actcttcacc ttccagtgta tagtggccgt tttccccaac
    14821 cgtgagtgtc agttgctgat cctgaccatt cagttgtgga atatgcatcc agctgatcgc
    14881 cagctcacct ggtttttctt tggttaatct cgcccagccg cgacccacaa tcggaaaata
    14941 cttctgctca actatgtcgc gcagattcag ttcagcaatg gttttaccga gaatcatgcg
    15001 cgattgcagc agttggatct ccggtgcaga ctcgggcgat gagttaggga tcatatcgct
    15061 caggccgctg agaatggcgt tgccctgttt ttgctcaacc tggaccagag tatctgcctg
    15121 ataaattggt gtgcttaaca gcgagtaagc gacagcgatc agcgtgaata acgcggtcac
    15181 gctgataata aacttacggt gatcccataa ctcgccgacc agacgaagca gatcgatctc
    15241 attttcctga gtgctgcctg gtggcgtatt catattttta gttgtcatcg tattttataa
    15301 actcaaatta ccggctgagg cgcttcgccc attcctgact ggcgcgctcc aacataccgt
    15361 agacatgttc aaatgcgtcc tgacttttac gatagggatc cgggatctct ttctgttcca
    15421 gccattgccc aaacagcatt gtcttcccgc ggacctcggg agcaattgcg gtaacctgag
    15481 caatatgttc cgactccatt gccagaatca gatcgtaatt tcgtgccatc tcagcagtga
    15541 gcttacgtcc ggcatgtcct tcaagagata cgccatgatt agcggcaaca tctgccgctg
    15601 tcgcatctgc agggtgtttt accagaccat gaacacctgc cgatttgact tttacgcccg
    15661 gcagacgttt acgcagcaag cgctcgccaa tgggcgaacg gcaaatgttg ccggtacaaa
    15721 ccaccaggat tgagttaaat tttagttggg ccatgtttga atccaccgtg aagtctctgt
    15781 caggtcatgc accccggaaa tcgtcggtac cagcagagaa atcacacggt tccaacgagc
    15841 cagcggcgcg gtagtgacat aaacaatgtc gtaaggttca agctggaatt ccgttccgag
    15901 tatcattgcc gatgcgtcct tcgcattcag ctgataaata ttggcgattt tgccattctg
    15961 cttgttttgc gttgcacgaa taacaaaaat ccccgtcgca tcagcgacat cctgattcat
    16021 accctcagcg ttacccagcg cttccgccag ggtcataccg ctgcggtcca ttttcagcgt
    16081 gctctgttta cccacttcac ccatcacgaa gactttcaga tcatcgttgc gcggaataaa
    16141 taaaatatcg cccgggtgca gcagtttgtt ctggcgcaag tcgccacgct gcatcagcgc
    16201 atagaggttc actttggtct ttacgccatt ctgggtgagc accacgttgc gccagtctgc
    16261 atctgcggtc agaccgcccg cggcgttaat cgcatccatg atggtcagag gaatattggt
    16321 aatcggctgc tgacccgatt tgctgacttc gccggtgacg tacgcttttt gcgagcggaa
    16381 cgccgcgacg ctgacatcga cttgcgggct ttcaatgact gaatccagac gcgctgtaat
    16441 ctcgttgcgt acctgggtaa gtgttttccc ggccactttc aggcgaccaa tataggggta
    16501 gaaaatcgca ccatcggcgt taacccagtt gccggtatca ctggcgctac ggtattgccc
    16561 tgcgggggtg gttagttccg ggtgatccca gaccgtaacc atcaacacat ccccaatgcc
    16621 gatacgatat tcatagtttg cgatttgctg ctccagctcc gggttagcct gcgacataat
    16681 cggtttggcg cgcaactgat caatcaatcc tggcgtgacg ggatagatat tgaccatttt
    16741 gtccaggtca tactgattgt caggtagttc aatgacatcc ttgttactgg tcgataaatt
    16801 ctgaccagga acaagggtac atgctgtcaa agacagaaca gccaatgtca gtaccgaaaa
    16861 cttaaaaatg ttctttttca ttggtttata tttatcagtc aatgtaatgt tatgaattac
    16921 gtcaggttat tgtccaaccg gtacttctcg ctctgccgtc attggataga gctggtattt
    16981 gcgtgacagc atttgtccac catcacgcgt caatggtgtc cacgaaacca ccgcgcggtt
    17041 gcggttaggt ccaatggtca tcaagtcgaa tggaatcgag atataaaaac ctttactaaa
    17101 gccgccttcg ccgtagtcat ctttagatac gttactgatt gccgcccata cccctaccgc
    17161 cacgccgctg tcaaagcgtt tggcgacgtc gatcgttgcg cctttatctt ttgccagata
    17221 ttgcccaacg ctaagtttca tcagtacgcc attgagcgtc ggcgggttcc agtaagccgt
    17281 cacgaaacct gttggcgtgg aataatcggt gaaacgcatc atgttgtccc agtcacgttg
    17341 cttcacgtag ttaacgtcca cacccagcgc ccagctggca tctagcgggc gataaagcag
    17401 ctcggaaccg acaccggcgt acatcgtttc cagataaccg ccatacacct ggccatagaa
    17461 accattgcct aagtcggcaa agtagttcgc ctgcaagttg ttgagataaa cgtcattgcg
    17521 aacgtaatca cggatatgcg tgcgcacgcg gggcagggta gagtccgcgg gcaacagcga
    17581 agacttaaac ttgtcgtagt tgttgtaaat attggtgaaa ataccgccat caagcagcag
    17641 gtggtcggta aaccagtagc gggcactgga catcagcccc agctggaaca tatagaaatc
    17701 ttccgggccg cccagcgact gtgaaagtgt tgggttgaaa gagtagctaa agcgatcttc
    17761 acgaatacgg tagccccgac caaaggcaga aagatcttct gcttcaacac gttgttgttg
    17821 aagttgctct gattgaccag gcgctgtacc agccagctgt ttgcgcaggc tggctacgtc
    17881 ggtttcggta gtcaccatcg ccatatgctc gcgcttttgc gtcacgctaa tcttctcaac
    17941 gccttgcggc aggttattca ccagaatccg gttggcacga tcgaccgctt cgcgagaatc
    18001 acggtatttg tattgttgac cagacatata cagcgtctta tcgcgcagct gaatttccgg
    18061 tgcttcaaaa cctgcgttgt acttcagcgc ggtaagttgg ttcgctactg tggtgtactg
    18121 caatccttca gattcaggcg caggttgata tgccggtttt ggcgtatcgc gcagggcagg
    18181 gcgcagatcg ttgaaattgg tccgtaacgt gaagccaaac atcaacgtgt taccgcgttc
    18241 ataactcagg ttgagatctg cccagctggc agcgcgataa actgcgccga cgttgaaatg
    18301 gcttgcctga ggcagtttgc cagcgaaatc attctggtaa ttgttgccat cgtattcgag
    18361 tttcagacgc aggggattcc acggcgtttg atactcaatg ccgccaaaga tggaagccgg
    18421 gccacgaaag atatcgctaa agctgatatc gcccgcatcg tgagactctg cgcgatgaca
    18481 atatttatcg ctcacccggc aaaacgggtt ggtaatattg cccgcattac cggcgtagcc
    18541 ccatgccatc ccgagggtga aatcaaatgg ccccgcttgc ttgctggcca ccagatactc
    18601 accgtcaaac agacccgtac cagcaatatc acgtttacca aacgccactt gcggtagcca
    18661 gtaaccttct tcccacaggc gaagtttaaa atcgaatgat ttgtctttgt atgactgatc
    18721 gccgctgaaa tcttcccact ggctatattt gcgtgtgcgc acatccgtat aacgaatggt
    18781 gccttccagc catgggaaca gcgccacgga ggtggagtag aaccggtatt gatcgttatc
    18841 ccggtagttg acgctgaatt caccttccgg tgcgatgcgc gcatttggca tctgcaacaa
    18901 tcctgtgccg ccaaagtctg attgcgacga acccagcgga tccgggtagg ttaatacttc
    18961 agcatgacag gcactggaga cggcaatggc caggcagctt aaaagataag aattcttctt
    19021 catcagtcag gaacacgttg cgttaaaaca gacacaatct ggtcatttaa atcagcatat
    19081 ttttcgggca gtacatgcgc agaaaagccc aaccaaagct ggctgccagg cggtggttcc
    19141 acatgtcttt tattccacag ggcaacaggg gcgacgacag tctcaccctc aggcgtaatc
    19201 accataacgt tattcttgtc cgcgcctgca aggcgggggt gatcttgcag ataatcggta
    19261 acgctacggc ctgcctgcca gggcaattgc ccggccccag aaaccgcacc cagcagagta
    19321 atagtcaccg gacgctgtac ggtatagagc gtatagtccc cgaccaacgg gggattgctg
    19381 ttctcatcca ctcgtacaaa atcagggtcc agtttgaccg gtaagcgacc agtgatattg
    19441 agattgagta actgctggcg cacggattta atcgttgccg ccacatcgtc gtcagcttct
    19501 gcttcccagg aagcaagctg tgccatgaca tgttggtagt ctttaagcgc tttagctttc
    19561 gccgcagagt cggtcagcaa ggcaccaggc caccagaggc gatcgcgcag ttgcggttgt
    19621 gtcaccaact ggacaacatt ttccatgggg ccaacggaaa gtgtctgttg ttcgccaggc
    19681 agataaatag tcaccgttcc ttgcgcaaag gcatggggtg tcattacgta aagtacgctg
    19741 gcaatgaaat acgactgtaa tttattcatg gtgcgggttt aagaaacgtc atttctacgg
    19801 gaataacccc tgcgcctaac atttgccgac tttgacgaac ctggccagta gccgaatcga
    19861 cccagaatgt gttttgccac tgtttttctg gccgcgtgga agtcacctct tcctgccaga
    19921 cattacacag cacattgcga cccgcaatat tcagcgtctc taatccagca aaggaaaaag
    19981 tagaactcag ggtcgcagag cgaaaatggt tgtcttcact ccagagaata tcgcgcgtcc
    20041 agcggctgcc atcttttatt gccagcgcgt tacgcagagg gtcctgcccg gaatttgtca
    20101 cttccagcag attattatta agtttgacgg tttttaaaag acgtccattg tgggtaacca
    20161 gcatggcgtt atcctgggat aaccatttgc tttgttcttg ttctatatat cccagtacca
    20221 caaagattcg ttgcccttca ttaaggcgta aatacatcgt ggaataaggc aacgcctgga
    20281 tctgctgatc cgctacggtg atatcctgat tatcgaaaag gctggcgcga aatgtatcga
    20341 ccatactttg ctggctgtgc gtacatcccg caagaaatag tgcgaaaatc gataaaataa
    20401 gagggcgcac ctttaatcct tgaaaggtta attaaaaaaa tgcaaatctc taaagcccgg
    20461 tacgaatatc gcaaccgggt atcatttaac tttaatttcg acgcgttaaa gcagtattag
    20521 cgtgtagtgg acgtggtggt tgaggtgtta tgagaaccgc catcgccact gtcatttgct
    20581 gcaacaactg ccaacgtcgc cacaccaaca gccgttgcca caccgacaac tgtcgccgtg
    20641 ccgacagcgc cagcagccac gccaccagtg gtagtagcag tcgtggttgc gccggtagtg
    20701 gtcgttgtgg tgccagtagt acccgtcgtt gcctcggtcc cgtttgttgc ttcaggggca
    20761 gcaaacgcag ctggcgtcgt caacatgaag gccataagga ttgcagaaag cttgtgtttc
    20821 ataacttttc ctttattcat cgcatggaca atacgtacgc ttgagatcag gattgtctac
    20881 tttgagtata acatccttgt gaaatctccc tttcctcata ccgcttgcac tatatactgc
    20941 gggtaggaaa ggcgcgcaga gggaaatata agattgttaa ctatccccct ctggaaatac
    21001 gacttgtgag cataagataa atcttagcaa aatacaaatt aaagataagg aaattgttac
    21061 gaaagctatt aattattgtt ggtaatatag tttcaagtgg tactatagct ggctgttaaa
    21121 tggcatttat aactattagg tgcagagata ttcgcttaaa tggattagtt tctttgtgga
    21181 aaagtaactg gtgttataat gtatgatgtt gactattgca ctattccaat aaaaccagtc
    21241 agctttaaac aagcagcgtc atattaagag agataaacat ttgccgctgt tggtcctcgc
    21301 aggccattta cgcggcaaaa ttccacacgt aatcctggta taagcacttc tgcgtcgcgg
    21361 ggagtgaatg cggaaatatg gacctgaact tctttacgac cgtcggaggg gataatgaat
    21421 cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa
    21481 attccttact gaaaatactg cgctgcacta tacggggtta ataaaataaa gccagcgata
    21541 tttaagaccg ccggacggct aaaataaaat ttgcttaatc tcaattatca tgcgttaata
    21601 gctgcgtcgg tttgaaagac agacagcata caaagtagtt tactaaagca gttctcatta
    21661 tcaggcatta tccccttctt ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa
    21721 agccctgttt gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg
    21781 gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct gatgatggca
    21841 gcaaagacgt tttcgtccat ttcactgcca tccagagcaa tgaattccgc acgctgaacg
    21901 aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg gcagcgaacg
    21961 ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg tccatgttgt
    22021 catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc tttgggcgct
    22081 aagtgtattt tttgtaaatc gacgatgatc acctttgata acgtcgcgct gcaaatacgc
    22141 acggaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg gagccttttt
    22201 tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc tgttgattat
    22261 tgaatctttc ggggagatgg cttataacat ttcttacctg accagggtac cgggaaccaa
    22321 caccttactg gcgtgttgct gtctttcaag accagaagag gttaacagtg aatattgaag
    22381 agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa atcgccgagc
    22441 tgaacaagaa tacagggaaa gaagtctctg aaattcgctt caccgcacga gaaaaaatga
    22501 ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa catcacccgt
    22561 gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct tgtggaatta
    22621 agcgtcggta caattcatcc ggtaccgggc tttgccatac tcccgcatac attgcgtaac
    22681 caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca gacaacgcaa
    22741 aggctttttt atttgccgca ggtttttgca gtgaaaaggt caatgttgag gctaccggac
    22801 atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga attaatttat
    22861 cgaccgggat ccgtgatggg caattttttg cgcatttgcc acagtcgata caactttcgg
    22921 cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc gcgccatatg
    22981 ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc aaaacggtca
    23041 cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta acgtccatca
    23101 ccacgctgta gggcgacaac ataaaatagt gaatcgcctg agcgggcatc aataatgcga
    23161 tatagagaaa aaaactcaac agcaaatact tcacgccgcg cagaggaata tccagccagc
    23221 ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt aattcagaaa
    23281 gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga ctgatgacga
    23341 taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga cctgttgtaa
    23401 ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata cctcccggtc
    23461 tggcgacaaa tgtcgtgcta cttgccgttt cgtaatagcg cacccaatac cagaacgtga
    23521 tggcaatata aatattcatt gccaacagta ataattgcgt cgctttacgc caggtcgtgg
    23581 cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc
    23641 gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac caaatgccac
    23701 tattctagtt gttcttaact ggctgatatt gattcaaatc gcgttcaggt ctttcttatt
    23761 caaccatgct tccagatcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga
    23821 taatggctgc tgctccagtt gtgcgcatag ctggctggcg atacgcattc ccaaactcga
    23881 gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctatcct gactggcgcg
    23941 ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt gtaaataata ccagccattc
    24001 gtggatcttc tccgtcccca ttaactgagc atcttcattg agttgcgata catccagcga
    24061 ttgatcgtta ttgacttgca gttggagata gtgcgccagt aactgaccga gcacttcacg
    24121 cggcaccggt ttagggataa tcccgcggaa taatgaactg gtacgctggc gcagcgtttc
    24181 gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg
    24241 tcgggcaagc gtaatgccgt cgacatccgg cagatcaaaa tccaccagtg cggcagcaaa
    24301 cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaacag caacaacctg
    24361 cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt tatcttcaat
    24421 taacagcaaa cgtaaaccgt caagacgcac cgcctgattg actgtttttg gcacgggtgc
    24481 cgtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt
    24541 ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca gccccagccc
    24601 ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg ccagtttcgc
    24661 gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc catcagtgcg
    24721 actacgcagg ataatgtacc cttcgtcagt aaaacgcagg gcgttgctca acaggttggt
    24781 tataacctga cgaatacgtc gtggatcgcc cattaacgcg cacggcatat catcggcaat
    24841 tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt gcagggtact
    24901 ttccagcagc gggcgcggtt caaagggctc atcgctgacc gaaacattct tgccacctgc
    24961 ttcgatagcg gaataatcga gaatatcgtt gaggatggtc agcaacgatt caccagagtc
    25021 agtaattgcc cgcaaatcat cacgctgggc gttaagtgcg gggttatctg ccagcagttg
    25081 agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa
    25141 cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac
    25201 cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg
    25261 attcagtgca tgaacattgc tgcgaaacgc atccatcagc cgcccgatgg tatccagctc
    25321 ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca gccgttgcag
    25381 cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga taaaccacgc gccagaggat
    25441 cagaatcagt gcgcaaagag aaactattcc cagcaataac aggctgtatt gcccgcgtgc
    25501 actcgctttt tccagatgcg ccagtccgtg ctgattacgc agctcaatgg tatcgaccag
    25561 ctgactgact tcgctactaa actgcgcgaa ctgggcgatg ttattttggg cgagagtttg
    25621 caggtgagtg ctgatttcac tgtcctgctg atacagcgcc agtagatcgc tatattggct
    25681 aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa tgcgtatttg
    25741 ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg gtgcattttt
    25801 ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag cgctaaggcg
    25861 cagttcattc atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact
    25921 ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct gggtcgctcc
    25981 agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc
    26041 agcgactatc tgctgactga gttgccgttg ttgctggcgt agttgcagac gccgccccac
    26101 cagttcccct tgctgacgta atgaacggga gatctcctgc tcctgttgtt caatagcggt
    26161 ggtatcaaaa ccttgttccc gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc
    26221 ggtgagcatt cgcccctgcg cctgccacat cttttcgtta tcggcactgg tcaggttctg
    26281 cgcggcgaaa agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg
    26341 aatcaatgcc tgagtgttgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc
    26401 caccagggta ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg
    26461 ggtcagggtt aaattcacgg tcggtgcact taaggtgaat aagttgtata tttaaaaact
    26521 cttttattat caatgaatta atgtaagtgg gtcattgttg gtcaaaataa aatcatttgt
    26581 cgatttcaat tttgtcccat tcccgtccac ggctgtccct ataccgcgcc gccattaaat
    26641 ctgatttatg cccgagaaga cgttgagcaa acttatcgcc aatctggttc cggtatagcc
    26701 tcgctgacag gctacgcagt tcatggaatg ttggcgggtt tccatcaaat gagagtccag
    26761 atgcatttct cgcctttgta aaatactttg atactgtttt cggggaaagc ggatcgtgat
    26821 gctttgatgc gattatagtt tcactgctgc tggcctccct gcatttctgt agtgtatcag
    26881 ccaatgagat attgagcgcg tcaatcgtta gcgttagcgg aatggcgagt ttagcccctg
    26941 ttttactctg ttcaatgtga agatggttgt cgtttatgtc tgaccatttc attctgcaca
    27001 aatcgccgac tctctgccct gtaacgacgg ccaaatccat cgccagcctt agccagatag
    27061 ggagaggttc ggctgcatgg taaatctcga catactcatt agctgtcagc cttgagcgcc
    27121 ttacttctga ctttgctgta cgggttgctg ttaccggatt cgttgcaaca tgcccctcgg
    27181 ctattgcttc acgaaaaacg tcaacaaggg ttgacctgat taattttgcg gaagctgctt
    27241 taccttctgc cacgtaggtg tttagcattg ctgccacttc tttcgttgat atgtcagtga
    27301 gcggtttgtc cggcaatttt cttcggattg ccctgatttt gctggcgtag tcgagtagag
    27361 ttttcggcct gatccctctt tcggtgagga ttctttcata tcggtcaagc cacacatgaa
    27421 gagtgattgc gtcaccgcct ttaattctgt ctatcagtga tttgcgtccg ctgtctgaga
    27481 gtaactcaat attggcctgt attgcttcag tgattgctat cctcctgtct cggcctaatc
    27541 caaactcttt acccgtcctt gggtccctgt agcagtaata tccattgttt cttatataaa
    27601 ggttaggggg taaatcccgg cgctcatgac ttcgccttct tcccatttct gatcctcttc
    27661 aaaaggctac ctgttactgg tcgatttaag tcaaccttta ccgctgattc gtggaacaga
    27721 tactctctgc catccttaac cggaggaggg aatatcctgc attcgcgcac ccatcgacga
    27781 actgtttcaa ggcttcttgg gcgtcgctgg cgagcgttcc actcctgaag tgtcaagtac
    27841 atcgcaaagt ctccgcaatt aca
//
LOCUS       DAZFKM010000061        27380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFKM010000061 DAZFKM010000000
VERSION     DAZFKM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHKAY_002624"
     CDS             complement(61..450)
                     /gene="mutT"
                     /locus_tag="ACHKAY_002624"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO5701284.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHKAY_002625"
     CDS             complement(510..3215)
                     /gene="secA"
                     /locus_tag="ACHKAY_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO5701285.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHKAY_002626"
     CDS             complement(3277..3789)
                     /gene="secM"
                     /locus_tag="ACHKAY_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO5701286.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHKAY_002627"
     CDS             complement(4020..4937)
                     /gene="lpxC"
                     /locus_tag="ACHKAY_002627"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO5701287.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHKAY_002628"
     CDS             complement(5038..6189)
                     /gene="ftsZ"
                     /locus_tag="ACHKAY_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO5701288.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHKAY_002629"
     CDS             complement(6250..7512)
                     /gene="ftsA"
                     /locus_tag="ACHKAY_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO5701289.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHKAY_002630"
     CDS             complement(7509..8339)
                     /gene="ftsQ"
                     /locus_tag="ACHKAY_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO5701290.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHKAY_002631"
     CDS             complement(8341..9261)
                     /gene="ddlB"
                     /locus_tag="ACHKAY_002631"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5701291.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHKAY_002632"
     CDS             complement(9254..10729)
                     /gene="murC"
                     /locus_tag="ACHKAY_002632"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO5701292.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHKAY_002633"
     CDS             complement(10783..11850)
                     /gene="murG"
                     /locus_tag="ACHKAY_002633"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO5701293.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHKAY_002634"
     CDS             complement(11847..13091)
                     /gene="ftsW"
                     /locus_tag="ACHKAY_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO5701294.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHKAY_002635"
     CDS             complement(13091..14407)
                     /gene="murD"
                     /locus_tag="ACHKAY_002635"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO5701295.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHKAY_002636"
     CDS             complement(14410..15492)
                     /gene="mraY"
                     /locus_tag="ACHKAY_002636"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO5701296.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHKAY_002637"
     CDS             complement(15486..16844)
                     /gene="murF"
                     /locus_tag="ACHKAY_002637"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO5701297.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHKAY_002638"
     CDS             complement(16841..18328)
                     /gene="murE"
                     /locus_tag="ACHKAY_002638"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO5701298.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHKAY_002639"
     CDS             complement(18315..20081)
                     /gene="ftsI"
                     /locus_tag="ACHKAY_002639"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO5701299.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHKAY_002640"
     CDS             complement(20097..20462)
                     /gene="ftsL"
                     /locus_tag="ACHKAY_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO5701300.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHKAY_002641"
     CDS             complement(20459..21400)
                     /gene="rsmH"
                     /locus_tag="ACHKAY_002641"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO5701301.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHKAY_002642"
     CDS             complement(21402..21860)
                     /gene="mraZ"
                     /locus_tag="ACHKAY_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO5701302.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHKAY_002643"
     CDS             complement(22462..23466)
                     /gene="cra"
                     /locus_tag="ACHKAY_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO5701303.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23548..23634)
                     /locus_tag="ACHKAY_002644"
     CDS             complement(23548..23634)
                     /locus_tag="ACHKAY_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO5701304.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHKAY_002645"
     CDS             complement(23646..24137)
                     /gene="ilvN"
                     /locus_tag="ACHKAY_002645"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO5701305.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHKAY_002646"
     CDS             complement(24140..25864)
                     /gene="ilvI"
                     /locus_tag="ACHKAY_002646"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO5701306.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25983..26027
                     /locus_tag="ACHKAY_002647"
     CDS             25983..26027
                     /locus_tag="ACHKAY_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701307.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHKAY_002648"
     CDS             complement(26182..27126)
                     /gene="leuO"
                     /locus_tag="ACHKAY_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO5701308.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6551 a         7796 c         6565 g         6468 t
ORIGIN      
        1 acagcgtcgc atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac
       61 ctacagacgc ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt
      121 aagaccgact agcgacatcc actcaccggg ttgcccttct ttaccccacg gctccccttc
      181 ccagctttcg accagccaga accacagagt tatatgcctg tccgggaatt catattccag
      241 tttttcaaat agcgaaaaat gttgtggggt aatcccgact tcttcctgaa gttcacgcac
      301 caccgcctgt tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt
      361 cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt tcattgttct cgttgcgaat
      421 aatacctacc gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat
      481 cctgcgcctt ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat
      541 tttttaccag aaccgcacgg gcaaggatcg ttacgtccta ctttgcgctc accggtttgc
      601 gccgccagtg cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgc
      661 gctaaacgct cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata
      721 cgtacctgga ctttgctcag cgtgctgata acttcatatt ttaacgactc cagcatcgct
      781 gcaaacatgg agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca
      841 cgcaggtgga taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag
      901 tcaagcgttt gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact
      961 tcttctttac gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct
     1021 tcatgcagtt ctggttcttt atccagccac tcggcaattg gtaaatcgag gtcgaaatcg
     1081 ttcttcagac gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga
     1141 atgtaggcat caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc
     1201 acatcgctga catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta
     1261 gccacgtcat catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta
     1321 cgctgggcgt tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc
     1381 atacccagtt tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca
     1441 tcttccatcg acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg
     1501 cgcaactggt tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca
     1561 cctgcttcca gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc
     1621 tctacggtcg gattttccag cgcggcaact tctgcctgcc agctaccacc gagcacaata
     1681 tctgtaccac gacccgccat attggtcgcg atagtcaccg cagccggata acctgcctga
     1741 gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca ggacgttgtg cttaataccg
     1801 gctttggtca gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc
     1861 accggctggc ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc
     1921 gcttcagtca tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga
     1981 acaacgacgg tatccagctt gtagatggag ctaaattcga aagcttcggt atcagcagta
     2041 ccggtcatcc ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc
     2101 agcgtttggt tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga
     2161 ccatcggacc agcgacggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact
     2221 tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca gcgcatgagc gcgcagcgcc
     2281 gccgttacgt ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc
     2341 atgatgccct ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc
     2401 acctggcgag atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct
     2461 tctttttcct gacggatcag gtgcggaata attttattca cgcgtttata catttccgag
     2521 ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag
     2581 tccacttcgt ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac
     2641 gccatattgt cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca
     2701 gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accgacagtc
     2761 aggccaagga attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg
     2821 ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt
     2881 gcggtcaggg ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc
     2941 ataccgccga gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca
     3001 cgtaccacgg cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgt
     3061 gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg agagtttttc catctccggt
     3121 tccatggcat tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga
     3181 ctaccgaaaa ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt
     3241 gcggagttaa taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc
     3301 acggatgcct tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa
     3361 atgcgcataa tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc
     3421 gctgagcgta tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac
     3481 gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg attaccgtgc gaatggcatg
     3541 ttgatgccag taatcaacgg aatagttcga attcgggcgg cgtgtgttcg cttccagcaa
     3601 ggccaattga ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc
     3661 gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcaaccat
     3721 ccctaataag agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat
     3781 tccactcacg ttattgccat cccgatcgta ttcaaacgta tttagcgcac aaatcttatc
     3841 attaaagcct gcagtccaca gcacgaatga agataagttg tgcggaaaag tgcttaagaa
     3901 aacagcgttc gcaccgtttt ttatagctct tataaaattt aaaaaaaaac gactggttca
     3961 cctggccgga gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt
     4021 tatgccagta cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag
     4081 gtcacatatt cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt
     4141 gcatgaccgg atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag
     4201 tcaccgatcg catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct
     4261 tcgttcagta cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac
     4321 aaaccacggg actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg
     4381 atctggcgca taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca
     4441 atagccggat ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat
     4501 tcagcccact tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg
     4561 gcgcagttca actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg
     4621 ccgtccatga tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccaag
     4681 cccgcgagag cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc
     4741 agacacgtac agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt
     4801 ggattcaagt cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc
     4861 agggtgactt tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt
     4921 gtcctttgtt tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat
     4981 acattcggcg ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta
     5041 atcagcttgc ttacgcagga atgctgggat atccagataa tccggctctt tcgcagtttg
     5101 cggcgcattg tcattcacga ctttagcaac cggcttctgc tcctgggtca gcggagccat
     5161 cccatgctgc tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt
     5221 gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc
     5281 attcatatcc gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc
     5341 acggatggtg ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat
     5401 gttaaccagc acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat
     5461 agccatttca gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat
     5521 tgcgtagccc atctcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc
     5581 cggacgagta atcagttcag cgataccttg cacagcgcct ttcagtacat cgttcgctgc
     5641 gccaaacgca tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg
     5701 gatagtgatc agagagtcca catgcttgga cagttcagtg atcccctgct ccgcgaatgc
     5761 catacgcttc ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc
     5821 caaatctttt gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat
     5881 acccgcagca ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc
     5941 ctcatcagcc gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat
     6001 accgctaccg atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt
     6061 atttaccgcg aagaattcaa caccttcaat gcgctcgcgc accatgtgtt caacagcatt
     6121 accgccgccg ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg
     6181 ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga ggccgtaatc atcgtcggcc
     6241 tcataaaaat taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac
     6301 tgatgctgta acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata
     6361 gtgaagcaat cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt
     6421 aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc
     6481 aagaccttcg atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt
     6541 aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag
     6601 cagctcggta tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg
     6661 cggacgacca cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag
     6721 cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta
     6781 agcgatatca ctggtcacga cattgccagc ataaggaatt accttagtgt ggcgcaatgc
     6841 cccaccggta taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc
     6901 cagttcacgt tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag
     6961 ttggtcaact ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt
     7021 gtgacatgtg atcaggtgca cttttgcctg catccgcacg cccgaaagtc ctaccggatt
     7081 cttgatccct tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg
     7141 ctcatcgcgc acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt
     7201 cacttcttct tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc
     7261 agaaagcgcc agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat
     7321 ggcgcgttgt acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc
     7381 acgtgacggg cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc
     7441 tactaaagcg gcaaccttcg cggtaccaat ctccagtcct actaccagtt ttctgtccgt
     7501 cgccttgatc attgttgttc tgcctgtgca tgattttgtt gctgagtaga ttcctctggc
     7561 ggcaagggcg cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc
     7621 cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgagcc
     7681 aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac
     7741 tgccaggaac gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc
     7801 atctgcccca tttcgcgata gccctgcaac acttcattgg cgctgccttc cgggccatac
     7861 agcattggaa gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc
     7921 gcgtctacca tatgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc
     7981 ttcaattcat caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt
     8041 tctatttgcg tctggatgat gttgacatct tgggtcataa aggtacccgg ctcacccaat
     8101 gccaggatcg actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc
     8161 agctttgaga gcggcaggcg ttgcgcatct tccatccagc ccaacacgac ccagccgctc
     8221 accaacactg tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta
     8281 ttgcggcgag aagaaacctc ttcttcgcta tttcgcgtgt tcagagcagc ctgcgacata
     8341 ttagtccgcc agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg
     8401 tgccgccatc ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag
     8461 ataaaactgt ccatcgctgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa
     8521 cgtcgtccat gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc
     8581 tgcggggcag aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt
     8641 tccggacggt tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa
     8701 ctccggccca cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct
     8761 taatgcatct tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc
     8821 gcggctcggc ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc
     8881 gctcaggcct ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc
     8941 ggcaccttgc cacagcagtt tgctgcgtag tttatccatt gaaagcgcag atgccatcac
     9001 tccgctgccg gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc
     9061 accgccgcga ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt
     9121 cacgtcgact tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc
     9181 taacaccgct gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag
     9241 gaccgcgatt ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt
     9301 cagctaaaga acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt
     9361 taccggttaa taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg
     9421 gatcaatttt cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa
     9481 ttggcgcttc gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca
     9541 cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca
     9601 ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg
     9661 tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct
     9721 ccagcgggaa ttcaccgagg aaatcaaaac ggcgcccagt cccctggaag ctctccagcg
     9781 cacgtaaaat agcctcgtca tcaattccct cttccgtagc aaccgcaacc gcagctgcgg
     9841 cgttcagcgc gttatgacga cctggcgcat tcagggtgac gcgcatcggc tctttgtcct
     9901 ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt
     9961 cggcatcttc gctgaagccg taagtcgtgg tctgacgtcc cactcgcggt aacaattcgc
    10021 ggatcaccgg atcatcaaca cacatcaccg cacgaccgta aaacggcagg ttgtgcagaa
    10081 aattaataaa agtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg
    10141 cttcgatatt ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct
    10201 catctgcttc ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt
    10261 ttaccagccc gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa
    10321 ccatcgcggt ggttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac
    10381 gcattaactc agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg
    10441 cgacaatttc cgggttatag gcagaaatgg cgctggaaac aacgaccacg ctggcatcac
    10501 gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc cagattcatt aactgctgcg
    10561 tgaccggatt tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt
    10621 cggcaatacc gcccataccg gcaccaccga tgccgacaaa atgtatgtgc cgaacgcgac
    10681 gcatttcggg cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca
    10741 ttaacttctt aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg
    10801 gctcacttca tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc
    10861 tgccatggtt aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac
    10921 gctaagctgt ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt
    10981 ccagtattgc tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc
    11041 ggcgatttca ctcaccgtta aagcaccgga gcggcaaacg acgacatccg cccacgcata
    11101 cgccgccgcc atatcatcaa taaattccgt cactttatgc tgcggttgcc ccgcttcggc
    11161 atacgcctgt tcaacggatt gttgcgaacc tttaccgctc tgatgccaga tagtgactga
    11221 atcacccagt ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga
    11281 accacccact accagcacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa
    11341 cggcagcgcc agtacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc
    11401 gcctggaaac gcctgcatca ctttggtggc aatcttcgcc agccatttat tggttaagcc
    11461 cgcaataccg ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc
    11521 acctggacct gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat
    11581 aatcgcccgc gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt
    11641 tatacctttt ccacgcagac cagagatacg aatgaaatca atttcgatgc catgttttgg
    11701 cactaagtcc gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc
    11761 cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat
    11821 caccattaat cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc
    11881 tccagacgcg tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt
    11941 aagctcgaac caccgtaact gatcagcggc aatgtcagac ctttggtcgg taacatcccc
    12001 gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg
    12061 agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc ggccaatcga catcgcgcga
    12121 aaagcgacga agaataccat taaaagtgcc agcaccacac cgacataccc cagttcttcg
    12181 ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc
    12241 gagttaccta aaccttgtcc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt
    12301 aactgatagc cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg
    12361 atacggtacg gttcggcgag tatcagcaac acaaccgctg aaatgcccat accgataatg
    12421 gcaatgaact gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac
    12481 aacaccacca ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc
    12541 acgcccatcg gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc
    12601 agatagttgg cgatataaca aaacagcgac agttttgtca gctccgcagg ctggatacgc
    12661 agcaaaccga gatcgatcca acgcgatgcc cctttaaccg agctacccac taccagcacg
    12721 atcatcagca ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc
    12781 atcggcagac gcagcgtaat gatcgccaga atgaatgcca ggatcagata gacgccatca
    12841 cgcttcgcga agaagaacgg atcattggtt aagcgttgcc ctatgggcat tgacgccgag
    12901 gtcaccatga taaagccaat cgccgcaagg ccgaaggtca gccacagtaa ggtgcgatcg
    12961 tacatgatca ggctgtcggt atctttttcc cgcgagccca tcacccagcc ttttagcgcc
    13021 gtggagatcc agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga
    13081 gataaacgca tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa
    13141 agttcttgaa ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct
    13201 gaacacgcgg agccagcaag cgcatcgcct gttccatagt ttcggtttgt tctgccactt
    13261 ccgggcgtag cgccgccagc tgcgcaccgt cacgaccaaa acagtacaaa cgcacgttgt
    13321 cgccattcag atagcgcgcc agtggactga agtccgccga tttaccatcg ccgcccagca
    13381 acaaatgcag cgtgccgtct acctgcaagc cattcaacgc cgcttccgta ctgccgacgt
    13441 tagtggcttt cgaatcgtta atccagcgta cgccattgtg ctccagcacc acttcaaagc
    13501 gatgcggcag accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggcaaaccag
    13561 ctgcgtctgc cagtgccagt gctgccagcg cattggtgta gttatgctgc ccggaaagtt
    13621 tcatctcttt cacgttcagc actttctcgc ctttcactcg cagccaggtt tctccctgct
    13681 gatgattcag gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatcagcac
    13741 cgcgaatcgg cattgttaag gcatcatcag cattgacgac acaaactttc gcattttcgt
    13801 aaatacgcaa ttttgccgca cggtattgct gcaaaccaaa cggatagcgg tccatatgat
    13861 cttcagtcac gttcagaatg gtcgccgcca ccgcctgtaa gctggaagtg gtttccagct
    13921 ggaagctcga cagttccagc acgtacagtt cgcactcagc atcaagcagc atcaacgctg
    13981 gcaggccaat attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca
    14041 ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg
    14101 cttcgcggca gaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc
    14161 ttaaggatgg atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca
    14221 gccattcatc attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc
    14281 caggcggtgt catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt
    14341 ccacgcagga aagcccagtg aggcccaggc cgataatgac gacattttta ccctgataat
    14401 cagccatgat taacgtacct tcagcgttgc cagaccaatc agaaccagca tcagcgaaat
    14461 aatccagaaa cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg
    14521 aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat
    14581 gacagaaagc gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg
    14641 tagcagtacg gcgataatgc ctaacgcacc acctaacgcc agcgaaccta catcgcccat
    14701 aaagacctgc gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc
    14761 cgtgcagaca atgaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc
    14821 aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt
    14881 cggcataatt gccaggccgt cgagaccatc ggtcaggttt accgcgttgc cagtacccac
    14941 aatgacgaag taagccagca gaatgtagaa cagccccagc tgcggcatca catctttaaa
    15001 gaatgggacc accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca gggcgaaggc
    15061 gatacccagc gcaatgaccg acatccagaa atacttccaa cgagcgatca accctttggt
    15121 gtctttacgc accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag
    15181 caccaccaac acgcaccaga cgtacggatt ggacgggtaa gcccacagca gtacggagat
    15241 cacaatcgcc gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg
    15301 tgattcagga ccgtcgttac gcaccacctg accaaaggaa agtttttgca aatgagcaat
    15361 catacgcggg cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg
    15421 aaacgtcaga taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag
    15481 ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcggcacta
    15541 cgtgaaccct taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc
    15601 gtaattaacg cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca
    15661 tggctttgtt tacccacgct taacacgcgg tcaataccag cagcttttgc cgcctcgccc
    15721 acctgtacat ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc
    15781 acgcggtagc ccggcatttc agccagtacc tgaacagcgg cggtcattga accgacattg
    15841 gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg
    15901 cctggaacag ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg
    15961 gacatggaga gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc
    16021 agcagaacat cgacgctacc ggttggggtt tgtagggtaa attccgtacc gtgcgaggtc
    16081 acatggatat tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact
    16141 ttgcgtgagc caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg
    16201 gcgataccgt tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca
    16261 agcgagccaa aaccttccag atgcgccgct gccaggttgt tgaccagcgc agcttccggg
    16321 cgagtcagac tcacagtcca ggctatttcg ccctgatggt tcgcgccaag ttcaataact
    16381 gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg
    16441 agattgcctg ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct
    16501 ttaacggagg ttttgccgga ggaccccgtc agagcaacca cgcgcgccgg aacttgctgg
    16561 cgaacccatg cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc
    16621 aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg
    16681 tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg caacaaacag gcagcccggc
    16741 gtcagttttc gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca
    16801 ccgttgagaa tatcggtaag ttggctaagg gttacgctaa tcatgcaatc acccccagca
    16861 gacgcgccac cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat
    16921 cctcatggcc tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc
    16981 aagtcaccgc ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg
    17041 ccagaatatc gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca
    17101 ccgccacgtc agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc
    17161 gatcgccacc acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct
    17221 gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc
    17281 ctggcgcagt gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttta
    17341 acagatcagc cagcgggtag ccaagtgcca gcagtgtcgc cagcgcgagc agcaggttgc
    17401 tgacgttaaa agcgcccatc agatggcttt caatttcgcc atcgccccaa cttgaactaa
    17461 agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt
    17521 gacagttcgg attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca
    17581 gccagcggcg gcccacttca tcgtcggcgt taacaatcgc ctgaccacaa tgatgttcag
    17641 aataaagcag ccatttcgcg gcttcgtagt gttccatatc accatgataa tcaaggtgat
    17701 cgcggcttaa gttggtaaag accgacgccg caaatttcaa tgccgccaca cggtgctgta
    17761 ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc gccctgatcc accagccccg
    17821 ccagctcatg ctgaacatcg actgccgaac cggtggtatt ttctgtcggg atcactttcc
    17881 ccagcagacc gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc
    17941 actgcgccag cagctgggta gtcgtggttt tgccgttggt gcccgttacg cctacgagac
    18001 gtaaattgtc agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga
    18061 gctggctgag atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct
    18121 cacctttcgc ctctgcaata atggcagtca caccttgcgc tatcgcctgc gggatatatc
    18181 gacgcccgtc cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac
    18241 ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa
    18301 gaaggtcgcg caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca
    18361 tttttatcgc ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc
    18421 atgatggcac caaagaccgg cgctgaaacg gcgccgccgt agtatttacc cgcctgcgga
    18481 tcgttgataa caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta
    18541 taagcaatat atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt
    18601 ttaatggcga tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc
    18661 acgctttcca tcatatgcac cacagtgcgg acaatggatt ccgggaagac acgttcaccg
    18721 ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata
    18781 gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag
    18841 gtggccctct ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg
    18901 accaacccca aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacgct
    18961 gaggacggca tcgctaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc
    19021 ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga
    19081 taaggaatgg tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatt
    19141 accaccatcg gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc
    19201 atcgcctctt tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc
    19261 gccagcactt caccggtgtt gacatccacc agcacggcgc taccagattc agccttgtta
    19321 aaggccaccg cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc
    19381 agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca
    19441 tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc
    19501 ttctcaacgc cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc
    19561 acttcgccgg acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc
    19621 agttttttga tgtagtccgc catgtcaggg ttcacctgac gcgccagata aataaagcgc
    19681 cctttcgggt tggcgttaat gcgggctgaa agctgatcca gcggaatatt gagcgcgtta
    19741 gccagcgcct tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg
    19801 tcagcccaaa tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta
    19861 atcatgccgc gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc
    19921 accagcatat ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc
    19981 gccaggagaa tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct
    20041 tcctgacgtt ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat
    20101 ttttgcacta cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg
    20161 gcgatccttt ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc
    20221 cattcaatgt ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa
    20281 cgggtatggt gcgccgtggt taccacagtc accgccgtca aaataatgca aatgaacagg
    20341 cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg
    20401 cgctcgtggc ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc
    20461 atgcattcgt cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca
    20521 cctcttcttc gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt
    20581 tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac
    20641 gcataaaacg tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc
    20701 cacccggggc cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac
    20761 tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt
    20821 tcaccggcgt tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt
    20881 cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc aaaacgctct tcaccatagg
    20941 ttttcaatac ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact
    21001 gcccacgggt tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc
    21061 gctcagcatc atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct
    21121 tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga
    21181 tggagaagcg cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga
    21241 tcgccagcaa acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc
    21301 gaccaaaagt cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt
    21361 catccagcag caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc
    21421 agtcgctccg ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt
    21481 tgatgccagg ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact
    21541 tcttttgtca gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca
    21601 tccatctgac attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg
    21661 ttcatgctcg acagacgcga taatttttgc tcgataattt cccattcagg tagggggtaa
    21721 agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca
    21781 agcagctgtt cccggtaacg ggtaggcact gataagcgcc ctttgctgtc gagattgact
    21841 aacgttgctc cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc
    21901 ccactgaaag gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc
    21961 gaggtaaaaa acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg
    22021 cgcagcaaat taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcaccgttt
    22081 taaaatgact cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca
    22141 ttttaggaca tatcttcccc ggttaacagt ccctgttgcg tctgtgtggc gcttgatgcc
    22201 agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat cggcaattca tctctgcatg
    22261 taacaaaata atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg
    22321 tgagttaatt aacgggcggg tttaaaagaa cttatcaata atagtcagga atagtcttat
    22381 ttactttaag gatattgatg tccattcccg tactctacgc gccagaggga aattcacctg
    22441 gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca ccgcggcgat agagattacg
    22501 tttaatgcgc gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat
    22561 ctccagcaca cgctctgcga catcgcggtg acgttgagcc actgcaagca ccgggcactg
    22621 taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt
    22681 accatcgcga cgtagcgtga catccatcac cccttgcaac aacgcaaacg acgttgtgaa
    22741 cagcgcctgc ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc
    22801 ccgctcatag ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaggcagt
    22861 acggaaacct tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag
    22921 caccgtctcg gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc
    22981 ggcaccaacc acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg
    23041 gaacgggtcg ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac
    23101 aataatggca tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg
    23161 ctgatcttct gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgctcaag
    23221 atagttagcg atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga
    23281 acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg
    23341 caccacagcc atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc
    23401 gttaataaca tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag
    23461 tttcacaatt gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta
    23521 aattcattaa catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc
    23581 cactgatttc gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat
    23641 cattctcaac gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact
    23701 tccacaattt tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg
    23761 cctgctaatt gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt
    23821 tccgtattac gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg
    23881 atttcccgct caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc
    23941 ttgtgtaatt gcttttcgat ctgctcaagc actttttcat cgcccacggt ctggatggtc
    24001 atacgcgata atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcca
    24061 cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact
    24121 gataatatcc ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc
    24181 cgcccccgcg aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa
    24241 acaccaggcg attattgcac acctgttcca gcgcctcgct aagtttgctt tccagctcat
    24301 gcggatgaga gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg
    24361 gtagtgattg catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt
    24421 tcaccatccc cagatagcgg ttattgagat tcaccaccag taccggcaac tcgtattgca
    24481 acgcggtaga cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca
    24541 cggtttcttc tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg
    24601 tgccgaggcc accggaattg atccagcgac gcggtttgtc gaatggataa taaagtgcag
    24661 caaacatctg gtgctgcccg acatcggacg tcacgtaagc gtctcccttc gtcaaccgcc
    24721 aaagagtctc gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc
    24781 actgacgagc gcgccactgt tcaatttgct gccaccagtc gcggatctca tccagtggtt
    24841 gatgggcgga ttcttgcgac aagagttcaa gcatttgttc gaggacctgg cgagcatccc
    24901 ccacaatcgg gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat
    24961 gcagaacagt ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc
    25021 gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac
    25081 cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccagcccca
    25141 tcaatgagga aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc
    25201 agcccgccat gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg
    25261 tttgcagagc gcgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca
    25321 tactgaccga ctcaggccag acatagggta atttgttcgc cggattaaga atatctttcg
    25381 gtaaatcaac gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca
    25441 gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg
    25501 aaatccccac catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct
    25561 gcccggaaag gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg
    25621 cattggtcgc ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcgcgcg
    25681 ccagaccatc ggccatatgc actgccgcct gctcatgacg aactaaaaca tgatcgatac
    25741 cacccacggt atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata
    25801 cttgtttaac gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct
    25861 ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct
    25921 tgcttaagca attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa
    25981 aggtgaaaag aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct
    26041 gaatcccaca acttacagca atttctctat ttttttaatt aatcctaaaa cagagagccc
    26101 acacattcag aataaaattc tacattcaat aaaaaataca gaataaacca gacattcatg
    26161 cctgacctat tctgcattca gttagcgttt gcaaattgag actaattgct cttccatcca
    26221 ctgatggcct ttatcgcgcc cggcagcttc atgccaggag agataacagg ttctgctatt
    26281 ttgttttaac ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag
    26341 ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat
    26401 tgccatgccc tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact
    26461 aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt
    26521 cagtaacggg cccttaattg ttggatgatt tttgctggct accagcacca tttcatcttt
    26581 aaataatggc acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc
    26641 cgtttcctga taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac
    26701 atgtatattt ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc
    26761 taacgggctg caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga
    26821 accaggcaat tcattttgta ctagttgcaa tgcctgacga actgagccaa aaagttgaaa
    26881 tgcgcgagca gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat
    26941 caccttcagg cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc
    27001 ggcacgggta atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc
    27061 gaccatgcgt agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc
    27121 tggcatactt aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa
    27181 taatcctgaa gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa
    27241 ttcggaaaac ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt
    27301 catttaccac agcataataa tccataatgt ttttgttgcg aaaacaatct aatcatgact
    27361 accgcgaata ctcaatcatc
//
LOCUS       DAZFKM010000062        26760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFKM010000062 DAZFKM010000000
VERSION     DAZFKM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..85
                     /locus_tag="ACHKAY_002649"
     ncRNA           <1..85
                     /ncRNA_class="other"
                     /locus_tag="ACHKAY_002649"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            334..1377
                     /gene="nadA"
                     /locus_tag="ACHKAY_002650"
     CDS             334..1377
                     /gene="nadA"
                     /locus_tag="ACHKAY_002650"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO5701309.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            1415..2134
                     /gene="pnuC"
                     /locus_tag="ACHKAY_002651"
     CDS             1415..2134
                     /gene="pnuC"
                     /locus_tag="ACHKAY_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO5701310.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2131..3072)
                     /gene="zitB"
                     /locus_tag="ACHKAY_002652"
     CDS             complement(2131..3072)
                     /gene="zitB"
                     /locus_tag="ACHKAY_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO5701311.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3186..3566)
                     /locus_tag="ACHKAY_002653"
     CDS             complement(3186..3566)
                     /locus_tag="ACHKAY_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO5701312.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3882..4934
                     /gene="aroG"
                     /locus_tag="ACHKAY_002654"
     CDS             3882..4934
                     /gene="aroG"
                     /locus_tag="ACHKAY_002654"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO5701313.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(5093..5845)
                     /gene="gpmA"
                     /locus_tag="ACHKAY_002655"
     CDS             complement(5093..5845)
                     /gene="gpmA"
                     /locus_tag="ACHKAY_002655"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO5701314.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6048..7088)
                     /gene="galM"
                     /locus_tag="ACHKAY_002656"
     CDS             complement(6048..7088)
                     /gene="galM"
                     /locus_tag="ACHKAY_002656"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO5701315.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(7082..8230)
                     /gene="galK"
                     /locus_tag="ACHKAY_002657"
     CDS             complement(7082..8230)
                     /gene="galK"
                     /locus_tag="ACHKAY_002657"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO5701316.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8234..9280)
                     /gene="galT"
                     /locus_tag="ACHKAY_002658"
     CDS             complement(8234..9280)
                     /gene="galT"
                     /locus_tag="ACHKAY_002658"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO5701317.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9290..10306)
                     /gene="galE"
                     /locus_tag="ACHKAY_002659"
     CDS             complement(9290..10306)
                     /gene="galE"
                     /locus_tag="ACHKAY_002659"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO5701318.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10567..12039)
                     /gene="modF"
                     /locus_tag="ACHKAY_002660"
     CDS             complement(10567..12039)
                     /gene="modF"
                     /locus_tag="ACHKAY_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO5701319.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(12107..12895)
                     /gene="modE"
                     /locus_tag="ACHKAY_002661"
     CDS             complement(12107..12895)
                     /gene="modE"
                     /locus_tag="ACHKAY_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO5701320.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            13024..13173
                     /gene="acrZ"
                     /locus_tag="ACHKAY_002662"
     CDS             13024..13173
                     /gene="acrZ"
                     /locus_tag="ACHKAY_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO5701321.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13339..14112
                     /gene="modA"
                     /locus_tag="ACHKAY_002663"
     CDS             13339..14112
                     /gene="modA"
                     /locus_tag="ACHKAY_002663"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5701322.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14112..14801
                     /gene="modB"
                     /locus_tag="ACHKAY_002664"
     CDS             14112..14801
                     /gene="modB"
                     /locus_tag="ACHKAY_002664"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO5701323.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14804..15862
                     /gene="modC"
                     /locus_tag="ACHKAY_002665"
     CDS             14804..15862
                     /gene="modC"
                     /locus_tag="ACHKAY_002665"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO5701324.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15863..16681)
                     /gene="ybhA"
                     /locus_tag="ACHKAY_002666"
     CDS             complement(15863..16681)
                     /gene="ybhA"
                     /locus_tag="ACHKAY_002666"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO5701325.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            16836..17831
                     /gene="pgl"
                     /locus_tag="ACHKAY_002667"
     CDS             16836..17831
                     /gene="pgl"
                     /locus_tag="ACHKAY_002667"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO5701326.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17872..18825)
                     /locus_tag="ACHKAY_002668"
     CDS             complement(17872..18825)
                     /locus_tag="ACHKAY_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5701327.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            19009..20061
                     /locus_tag="ACHKAY_002669"
     CDS             19009..20061
                     /locus_tag="ACHKAY_002669"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO5701328.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            20137..21570
                     /locus_tag="ACHKAY_002670"
     CDS             20137..21570
                     /locus_tag="ACHKAY_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO5701329.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            21753..24014
                     /locus_tag="ACHKAY_002671"
     CDS             21753..24014
                     /locus_tag="ACHKAY_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO5701330.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(24248..25531)
                     /locus_tag="ACHKAY_002672"
     CDS             complement(24248..25531)
                     /locus_tag="ACHKAY_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO5701331.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(25666..26736)
                     /locus_tag="ACHKAY_002673"
     CDS             complement(25666..26736)
                     /locus_tag="ACHKAY_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701332.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTH
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
BASE COUNT         6371 a         6610 c         6932 g         6847 t
ORIGIN      
        1 tgagttgagc gcagcgaaac aacggagccg ctcgcggcga cggcccgaag ggcgagcgaa
       61 gcgagtcatc ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta
      121 ttcaggcatc ctcaatttca ctttgtaaac ctgatgacat cgtcagagct tactgtgcaa
      181 gcaactctac gtcggtggaa ttaggcgtaa ttcgagtgac ttttccccac cattcgacta
      241 tcttgtttag catataaaac aaattacacc gataacagcg aatattacgc taatgtcggt
      301 tttaacgtta agcctgtaaa acgagatggt aagatgagcg taatgtttga tccagacacg
      361 gcgatttatc ctttcccccc gaagccgacg ccgttaagca ttgatgaaaa agcgtattac
      421 cgcgagaaga taaaacgtct gctaaaagaa cgtaatgcgg tgatggttgc ccactactat
      481 accgatcccg aaattcaaca actggcagaa gaaaccggtg gctgtatttc tgattctctg
      541 gaaatggcgc gcttcggtgc aaagcatccc gcttctactt tgttagtcgc tggggtgaga
      601 tttatgggag aaaccgccaa aattctcagt ccggaaaaaa caattctgat gccgacactt
      661 caggctgaat gttcactgga tctcggctgc cctgttgaag aatttaacgc attttgcgat
      721 gcccatcccg atcgtactgt cgtcgtctac gccaacactt ctgctgcggt aaaagcgcgc
      781 gcagattggg tggtaacttc aagcattgcc gtcgaactta ttgatcatct tgatagtttg
      841 ggtgaaaaaa tcatctgggc acccgacaaa catctggggc gttacgtgca aaaacagacg
      901 ggtggagaca ttctatgctg gcagggtgcc tgtattgtgc atgatgaatt taagactcag
      961 gcgttaaccc gcttgcaaga agaatacccg gatgctgcca tactggtgca tccagaatca
     1021 ccacaagcta ttgtcgatat ggcggatgcg gtcggttcca ccagtcaact gatcgctgct
     1081 gcgaaaacat tgccacatca gcggcttatc gtggcaaccg atcggggcat tttctacaaa
     1141 atgcagcagg cagtgccaga taaagagtta ctggaagcac caaccgcagg tgagggtgca
     1201 acctgccgca gctgcgcgca ttgtccgtgg atggccatga atggccttca gtccatcgca
     1261 gaggcattag aacaggaagg aagcaatcac gaggttcatg ttgatgaaag gctgcgagag
     1321 agggcgctgg tgccgctcaa tcgtatgctg gattttgcgg ctacactacg tggataacga
     1381 ataataaggc gtaacgttac gctttggggg aaagatggat ttttttagtg tgcagaatat
     1441 cctggtacat ataccaatag gggcaggcgg ttatgatctc tcatggatcg aagcggtagg
     1501 cacgatcgcc gggttgctgt gtattggcct tgccagtctg gagaagatca gcaactactt
     1561 ctttggcctg atcaacgtca ccttgtttgg cattattttc tttcagattc agctgtatgc
     1621 cagcctgcta ttacaggtgt ttttctttgc cgcgaatatt tacggttggt atgcgtggtc
     1681 gcgacaaacc agtcagaacg aggcggagtt gaaaattcgc tggttgccat tgccgaaggc
     1741 actcagctgg ttggcggttt gcgttgtttc gattggtctg atgacggtat ttatcaatcc
     1801 ggtgtttgca tttttgaccc gcctggcagt catgatcatg caagcattag gattacaggt
     1861 tgtgatgcct gaactgcaac cggacgcttt cccgttctgg gattcatgca tgatggtgtt
     1921 atctatcgtg gcaatgattc tgatgacgcg taagtatgtg gaaaactggc tgttgtgggt
     1981 gattattaac gtgattagcg tcgttatttt tgcacttcag ggcgtttacg ccatgtctct
     2041 ggagtacatc atcctgacct ttattgcgct caacggcagc cggatgtgga tcaacagcgc
     2101 acgtgaaaga ggctcacgcg cgctgtccca ttaatggtga tgatgtgaat ggccagacac
     2161 cccttcattc aggtggcagt ctggcccatg acaaggttga tattccatct gaatggtggc
     2221 gtgctcaatc tgatagtgat ccatcaggta gtgttggatc tgatccaaca aggcatcgtg
     2281 atcgtgtggc gggatcacct gcacatgcaa cgtcatcacc ggcttctcgc ctaccatcca
     2341 tacatgtaca tggtgcacat tgcggacttc cgggatttcc cggcacatac gacgcttcag
     2401 ttcagcgata tccagcgata ccggtgcacc ttcaagtaat tcattcacgc tatctttcaa
     2461 cagtcgccac gcgctacgca gaaccagaag cgacaccagt atcgagagaa tggggtcagc
     2521 aggtgtccag ccagtccaga taataatcaa cgcggcaata atagccccaa ccgaacccag
     2581 caggtccccg agcacatgca gtgccgctgc tcgtacgttg aggtttttct cttcgctgcc
     2641 gtgatgaagt aaccagaaag aaagtatatt tgccagcaac ccagccacgg caattgccat
     2701 catcatgccg ccctcgaccg gacgcggcgt gcggaagcgt tctatcgcct cccagacaat
     2761 caaaatggta attaccacga gggctatggc attcacaaac gccgcgagcg tggtcagtct
     2821 tagccaaccg aaagtgtggc gaatggtggg aggacgacgt gaaaattgca cggcgagtag
     2881 ggcgaaaagc agggcggcag tatcggtcaa catatgaccc gcatcggcca gcaatgccag
     2941 agaaccagaa agaaaaccac caacgacttc taccagcata aacccggcag tcacgccgaa
     3001 agcatacaac aagcgacgag cattattatc ttctggcagg tgtgaggacg tgtgtgagtg
     3061 tgagtgcgcc atgacgagtc atcccttaat tattctcact taactttatg acatcatact
     3121 gcttttagaa gtgaaaaatt aaaagggaga gactccgctc tcccattatt ggctattttg
     3181 cagggttact gcgtggtacc gtcggttttg gtatcgacat cattattgat gccatcaccg
     3241 gtttgtacct ttttattgat atccggacag cgaccatctt tgcacatggt gttcttgtgc
     3301 tcttcatctt tggtcattcc gtcattgttc attgaagaac catccgaatg cagcattgtg
     3361 ccaccagaac cggtatttac cccgttattg tcgacgttat ttggcgcgac attttcacgg
     3421 gcgtcagggg ctacctggcc cgcatcagct gcggcgtttg cctggccgtt attagtttgc
     3481 gctccgctat cggcagccag tgcggcaccg ctggcaaggc ttagagtggc agtcagaaat
     3541 aatgtggcca gttttgtcat tttcatagga tgctcctgtt atggtcgtta tgtcggataa
     3601 cctcttccaa cagtgcattt gcaggtgaat ataaggcatt ggtttaagat ttcagccagg
     3661 ttatgaaacg cagcagagaa tcttgaaata attaacaaac aaaggagtta cagttagaaa
     3721 ttgtaggaga gatctcgttt ttcgcgacaa tctggcgttt ttcttgctaa ttccaggatt
     3781 aatccgttca tagtgtaaaa ccccgtttac acattctgac ggaagatata gattggaagt
     3841 attgcattca ctaagataag tatggcaaca ctggaacaga catgaattat cagaacgacg
     3901 atttacgcat caaagaaatc aaagagttac ttcctcctgt cgcattgctg gaaaaattcc
     3961 ccgctactga aaatgccgcg aatacggttg cccatgcccg aaaagcgatc cataagatcc
     4021 tgaaaggtaa tgatgatcgc ctgttggtgg tgattggccc atgctcaatt catgatcctg
     4081 tcgcagcaaa agagtatgcc actcgcttgc tggcgctgcg tgaagagctg aaagatgagc
     4141 tggaaatcgt aatgcgcgtc tattttgaaa agccgcgtac cacggtgggc tggaaagggc
     4201 tgattaacga tccgcatatg gataacagct tccagatcaa cgacggtctg cgtatagccc
     4261 gtaaattgct gcttgatatt aacgacagcg gtctgccagc ggcgggtgag tttctcgata
     4321 tgatcacccc acaatatctc gctgacctga tgagctgggg cgcaattggt gcacgtacca
     4381 ccgaatcgca ggtgcaccgc gaactggcgt ctggtctttc ttgtccggta ggttttaaaa
     4441 atggcacaga cggtacgatt aaagtggcta tcgatgccat taatgccgcc ggtgcgccgc
     4501 actgcttcct gtccgtaact aaatgggggc attcggcgat tgtgaatacc agcggtaacg
     4561 gtgattgcca tatcattctg cgcggcggta aagagcctaa ctacagcgcg aagcacgttg
     4621 ctgaagtgaa agaagggctg aacaaagcag gcctgccagc acaggtgatg atcgatttca
     4681 gccatgctaa ctcgtccaaa caattcaaaa agcagatgga tgtgtgtgct gacgtttgcc
     4741 agcagattgc cggtggcgaa acagccatta ttggcgtgat ggtggaaagc catctggtgg
     4801 aaggcaatca gagcctcgag agcggggagc cgctggccta cggtaagagc atcaccgatg
     4861 cctgcatcgg ctgggaagat accgatgctc tgttacgtca actggcgaat gcagtaaaag
     4921 cgcgtcgcgg gtaaggttta attgtcggat gcgccgtcag agtggcgtat ccgatgaatc
     4981 accacaggcc tgataagacg cgcagcgtcg catcaggcaa tgtgctccat tgttagcaac
     5041 aaaaaagccg gctcacttgc agtcggcttt ctcattttta aacgaatgac gtttacttcg
     5101 ctttaccctg gttagcaacc gccgctgctt tcgctgcgat ctcgtcagca ttacccagat
     5161 aatagcgttt cagcggtttg aaattctcgt cgaactcata caccagcggc acgccggtcg
     5221 ggatattcag ctcaagaatc tcttcttcac tcatgttatc aagatatttc accagcgcac
     5281 gtaaagagtt accgtgtgca gcgatgatca cgcgctcacc gctcttcata cgcggtagaa
     5341 tagtttcatt ccagtaaggg atcacgcggt caatggtcag cgccaggctt tccgtcagcg
     5401 gcagttcttt ctcgctcagc ttcgcgtaac gtggatcgtg acccggataa cgctcatcat
     5461 ctttagtcag ttccggcgga gtgactgcaa aaccacgacg ccactgtttc acctgctcgt
     5521 cgccatactt ttcagcggtt tctgctttgt tcagaccctg caacgcaccg tagtgacgtt
     5581 cgttcagttt ccaggatttc tcaacgggca gccatgcctg atccagttcg tccagcacat
     5641 tccacagggt atggatagcg cgtttcagca cagaagtgta agcaaagtca aagctgtaac
     5701 cttcctcttt cagcagctta cctgctgctt ttgcttcgct tacgcctttc tcagacagat
     5761 ccacgtcgta ccaaccggtg aaacggtttt ctttgttcca ctgactttcg ccatgacgaa
     5821 ccagaaccag cttagttaca gccatatact tactcctcaa atcatctttt aatgataata
     5881 attctcatta tattgccgtg acgaagcaac agcaatgctt acgcataacc atagcgaaaa
     5941 tagtggcgca gtgtaaggtt gttgtgaata ttgagttgca aatatgtcgg tgtttgctgg
     6001 tgatttgaac aatatgaaga taaagccctc atgacgaggg cttaacatta ctcagcaata
     6061 aactgatatt ccgtcaggct ggaatactct tcgccaggac gcaggaagca gtccggttgc
     6121 ggccattcag ggtggttcgg gctgtccggc agaaactcgc tttccaacgc cagcccttgc
     6181 cagtcggcgt aaggttcggt tccccgcgac ggtgtgccgc cgaggaagtt gccggagtag
     6241 aattgaagag ccggagcggt ggtgtagacc ttcagctgca atttttcatc tgctgaccag
     6301 acatgcgccg ccactttctt gccatcgcct ttggcctgta acaagaatgc gtgatcgtaa
     6361 cctttcactt tgcgctgatc gtcgtcggca agaaactcac tggcgatgat tttggcgctg
     6421 cggaaatcaa aagacgttcc ggcgacagat ttcaagccgt cgtgcggaat gccgccttca
     6481 tcaaccggaa gatattcgtc cgccagaatc tgcaacttgt gattgcgcac gtcagactgc
     6541 tcgccgtcaa gattgaaata gacgtgatta gtcatattca ccgggcaagg tttatcaact
     6601 gtggcgcgat aagtaatgga gatacggtta tcgtcggtca gacgatattg caccgttgcg
     6661 ccgagattac ccgggaagcc ctgatcgcca tcatctgaac tcagggcaaa cagcacctga
     6721 cgatcgttct ggttcacaat ctgccagcga cgtttgtcga acccttccgg gccgccgtgc
     6781 agctggttaa cgccctgact tggcgaaagg gtcacggttt caccgtcaaa ggtataacgg
     6841 ctattggcga tgcggttggc ataacgacca atagaggccc ccagaaacgc ggcctgatcc
     6901 tgatagcatt ccgggctggc acagccgagc agcgcctcgc ggacgctgcc atcggaaagc
     6961 ggaatacggg ctgaaagtaa agtcgcaccc cagtccatca gcgtgactac catccctgcg
     7021 ttgttacgca aagttaacag tcggtacggc tgaccatcgg gtgccagtgc gggagtttcg
     7081 ttcagcactg tcctgctcct tgtgatggtt tacaaacgta aaaagtctct ttaatacctg
     7141 tttttgcttc atattgttca gcgacagctt gctgtacggc aggcaccagc tcttccggga
     7201 tcaacgcgac gatacagccg ccaaatccgc cgccggtcat gcgtacgcca cctttgtcgc
     7261 caatcacagc tttgacgatt tctaccagag tgtcaatttg cggcacggtg atttcgaaat
     7321 catcgcgcat agaggcatga gactccgcca tcaactcgcc catacgtttc aggtcgccct
     7381 gctccagcgc gctggcagct tcaacggtgc gggcgttttc agtcaggata tgccgcacgc
     7441 gtttcgccac gattgggtcc agctcatgtg caacagcgtt gaactcttca atggtgacat
     7501 cgcgcagggc tggctgctgg aagaaacgcg caccggtttc gcactgttca cgacgggtgt
     7561 tgtattcgct gccaaccagg gtacgtttga agttactgtt gatgatgacg acagccacac
     7621 ctttcggcat ggaaactgct ttggtcccca gtgagcgaca gtcaatcagc aaggcatgat
     7681 ctttcttgcc gagtgcggaa attagctgat ccatgatccc gcagttacag ccaacaaact
     7741 ggttttctgc ttcctgaccg ttaagcgcga tttgtgcgcc gtccagcggc agataataaa
     7801 gctgctgcaa tacggttccg accgcgactt ccagtgaagc ggaagaactt aacccggcac
     7861 cctgcggcac attgccgctg atcaccatgt ccacaccgcc gaagctgttg ttacgcagtt
     7921 gcagatgttt caccacgcca cgaacgtagt tcgcccattg atagttttca tgcgcgacaa
     7981 tgggcgcatt gagggaaaac tcgtcgagct gattttcata atcggctgcc attacgcgaa
     8041 ctttacggtc atcgcgtggc gcacagctga tcacggtttg ataatcaatc gcgcagggca
     8101 gaacgaaacc gtcgttgtag tcggtgtgtt caccaatcaa attcacgcgg ccaggcgcct
     8161 gaatggtgtg agtggcaggg tagccaaatg cgttggcaaa cagagattgt gttttttctt
     8221 tcagactcat ttcttacact ccggattcgc gaaaatggat atcgctgact gcgcgcaaac
     8281 gctctgctgc ctgttctgcg gtcaggtctc gctgggtctc tgccagcatt tcataaccaa
     8341 ccataaattt acgtacggtg gcggagcgca gcagaggcgg ataaaagtgc gcgtgcagct
     8401 gccagtgttg attctcttcg ccattaaatg gtgcgccgtg ccagcccata gagtagggga
     8461 aggagcactg gaagaggttg tcataacgac tggtcagctt tttcaacgcc agcgccaaat
     8521 cgctgcgctg ggcgtcggtc aaatcggtga tccgcaaaac gtgggctttg ggcagcagta
     8581 gcgtttcgaa cggccaggca gcccagtaag gcacaacggc taaccagtgt tcggtttcga
     8641 caacggtacg gctaccgtct gccagctcgc gctgaacata atccaccagc attggtgatt
     8701 tctgttcggc aaaatattct ttttgcaggc ggtcttcgcg ctcagcttcg ttaggcagga
     8761 agctatttgc ccaaatctgt ccgtgcggat gcgggttaga gcagcccatc gccgcacctt
     8821 tgttttcaaa gacctgcacc cacgggtacg tttttcccag ctctgcggtt tgctcctgcc
     8881 aggttttgac gatttccgtc aatgccgcaa cgctcagttc tggcaacgtt ttactgtgat
     8941 ccggtgaaaa gcagatcacc cggctggtac cgcgcgcgct ctggcaacgc atcaaaggat
     9001 cgttgctttc tggcgcatct ggcgtgtcag acatcaaggc cgcaaagtca ttagtgaaaa
     9061 cgtaagtcct ggtgtaatcg gggtttttat cgcctgtcac ccgcacatta cctgcgcaga
     9121 ggaagcaatc tggatcgtgc gcaggtaaca cctgtttggc tggcgtttcc tgcgccccct
     9181 gccaggggcg cttagcgcgg tgcggtgaaa ccagaatcca ttgcccggtg agcgggttgt
     9241 agcggcgatg tggatgatca acgggattaa attgcgtcat ggtcgttcct taatcgggat
     9301 atccctgtgg atggcgtgac tgccagtgcc aggtgtcctg cgccatttca tcgagtgtgc
     9361 gcgttacgcg ccagttcagt tcacggtcgg ctttgctggc gtccgcccag taggccggaa
     9421 ggtcgccctc gcgacgcggt gcaaaatgat aattaaccgg tttgccgcag gctttgctga
     9481 aggcattaac cacgtccagc acgctgttgc ctacgccagc gccgaggttg tagatgtgta
     9541 cgcctggctt gttcgccagt ttttccatcg ccacgacgtg accgtccgcc agatccatta
     9601 cgtggatgta atcgcgtacg ccagtaccat cttcggtcgg ataatcgtta ccaaaaatcg
     9661 ccagcgagtc gcgacggcct acagcaacct gggcgatgta tggcatcagg ttattcggaa
     9721 tgccttgcgg atcttcgccc atatcgcccg acggatgcgc gccaaccggg ttgaagtagc
     9781 gcagcagggc aatgctccag tccggctggg ctttttgcag atcggtgagg atctgttcca
     9841 ccatcagctt gcttttgccg taagggcttt gtggtgtgcc ggtcgggaag ctttcaacgt
     9901 atggaatttt gggctgatcg ccataaacgg tggcggagga gctaaaaata aagtttttga
     9961 cgttagcggc gcgcatggcg ctaatcaggc gcagagtgcc gttgacattg ttgtcgtaat
    10021 attccagcgg tttttgtacc gattcgccga cggctttcag cccggcgaag tggatcacgg
    10081 tgtcgatagc gtgatcgtgc agtatctcgg tcatcaacgc ttcgttacga atatcgcctt
    10141 caacaaacgt tggatgtttg ccgcctaaac gctcgataac aggcagtacg ctgcgcttac
    10201 tgttacagag gttatcaaga atgatgacat catgaccgtt ttgcagtaat tgcacacagg
    10261 tatgacttcc aatgtaaccg ctaccaccgg taaccagaac tctcataatt cgctccatta
    10321 ggcttatggt atgaaataac catagcataa caaagatgcg aaaagtgtga catggaataa
    10381 attagtggaa tcgtttacac aagaatttag ccgtttttta tgcgcgatta agtgattata
    10441 aaacagaggg tttatgaatg attgcgcttt ttatctgaaa aaagaagcgg tttcatgcct
    10501 gcatgcgtcg aaccgttggc cggagagggc gcttaggccg cctccggcaa gatcagcact
    10561 accgactcaa tatatttttg tcagcacata gcgatagagt ccaccgtccg gcacgaactc
    10621 aaggcgatgg gtaatacagg caggcgcatc ttcagcgtgg tgcgaaacaa acaacaattg
    10681 cgtttcacct tcgctaatca gcacatcaac aaaacggcgg ataagctggc gattgagcgg
    10741 atcaagcccc tgtagtggtt catcgagaat aagcaacgtc ggatgtttca ccagtgcgcg
    10801 gacgatcagc gccagacgct gctgtcccca ggaaagacta tggaacggag cgtcagccgt
    10861 acgtttatca atgccgagaa tatccagcca ctgctgcacc agtttttgct ggcgatccga
    10921 aacggcctga taaatgccaa tcgaatcaaa atagccagaa agaatcacat tacgcacggt
    10981 agtgctgacc cggtaatcca gatgcaaact actgctgacg taaccgatat gctttttgat
    11041 atcccagatg gtttcgccgc tgccgcgacg tcgtccgaaa agcgtcaaat cgttgctgta
    11101 accttgcgga tgatcgccag taaccaggct taataacgtc gattttcctg caccatttgg
    11161 cccgacaatt tgccagtgtt cacctggatt cacctgccag ctaaggttat taagaatggg
    11221 gcgatcgtta taagaaacca cgccattgtt cagcacaatg cgcggttcgt tggcgggtaa
    11281 ggcgtgacgt gctgaaggtt catccggctc cggcagttgc acaccttcaa gctgttcact
    11341 atgcgccagt tgcgcgacga gtgcttgttg gagcagttcc tctttagcgc cagtttccgc
    11401 taacgtgcaa tccgccagca cgccagcaaa ctggacaaac tccgggatct catcgaagcg
    11461 attgagcacc agtaccagag taataccgga ctgatgtaac gaggcgagtc gctcagccag
    11521 ctgctgacgt gaggcaacat ccaggccatc gaacggctca tcaagaatca acaagtcagg
    11581 ctccgacatc agcgcctgac acagcagggt ttttcgcgtc tcgccagtgg aaaggtattt
    11641 aaagcgtcgg tcgaggaggg cggtaatacc gaactgctgc gccagttgca tgcaacgcgg
    11701 tgcatccttt acttcatcct gaatgatctc agccgtagtg cgtccggtgt catcttcgcc
    11761 agggccgagc atatcggtgt tattccgctg ccattcgtcg ctgacgagtt tttgcaattg
    11821 ctcgaaggag agacgagtga tgtgggaaaa ctggctttgc cgttcacctt tcaaaagcgg
    11881 aagttccccc gccagcgcgc gggccagggc cgatttcccg cttccattcg aaccgacaaa
    11941 cgcccaacta tcacccgcgt ttaacgttag ctgaggcaat tgcagcgttt ttgtgtcgct
    12001 aagacgaaac gtgccttgca aaatttgcaa cgatgacatt ttatatccca ttttgtgcag
    12061 cgattactga cagggatacg tgtttcataa caaattgtca acacgcttag cacagcgtgg
    12121 cgataatcac gctgtcggca ttaaagtagg ccgtgacatt ctgcccttgc tgaagagaag
    12181 tcgcttcatt taccggcact gtggcgcaca gtgtttgccc gtcgggtagc gccattaata
    12241 cttcgcactg ctctgcgccg cgctcaatat gactaataat acccggtaat tggttgtcgg
    12301 cgttttgtgc gaccgcctcg tcctgagtaa tacctaccca cggcgctttt agcaatatca
    12361 acacttcttt gccttcatcc agccccagac gcgcgccgct ttgtgcggta attgcgactt
    12421 tcaggcgtgt ttttccgtca gccagtaaga catcaacatg ctgttgaacg tcatcatgat
    12481 cgcgggcggt gatggtgccg aaccactggt tacgggcgct ggtttgcagt gaaaaacgtg
    12541 agatcgcggc cagcaggctg ttcagcggca gggcgtcatc gtcacttaac acatcaaagg
    12601 ctttttgctg gatttgcgcc agtaagtcat agagctgaat cagtcgctga ccatagcggg
    12661 tcagtactgc gccgccgcca cctttaccgc ctgttgcgcg ctcgaccaga atatgctcac
    12721 ttaactgatt catctcgtta atggcatccc aggcgctttt atagctgata ccggcatctt
    12781 tcgctccctg gctaatggaa ccggaaagcg caatgtgttt tagtagcgaa atccggcgcg
    12841 ggtcggcgaa taatttttgt tggagcttaa gggtgagaag gatttcggcc tgcataacaa
    12901 tgtcctggca aaagtcttat tgtgacggaa aacgaacgcc acgcaaagct gaccgcacaa
    12961 aaggggagtg cttttctgtg cttagcggtt agaatagtct catgactata tctggagttg
    13021 accatgttag agttattaaa aagtctggta ttcgccgtaa tcatggtacc tgtcgtgatg
    13081 gccatcatcc tgggtctgat ttacggtctt ggtgaagtat tcaacatctt ttctggtgtt
    13141 ggtaaaaaag accagcccgg acaaaatcat tgattccctg aatgcccgct tagtcgggca
    13201 tttctttttc tcaacttcct gcttttccag ccgatatttt ttcttatcta cctcacaaag
    13261 gttagcaata actgctggga aaattctgag ctagccgtta tattgtcgcc tacataacgt
    13321 tacattaagg ggttaccaat ggctcgtaaa tggttgaact tgtttgccgg ggcggcactc
    13381 tctttcgctg ttgctggcaa tacactggca gatgaaggga aaatcacggt gttcgccgcc
    13441 gcatcactga ctaacgcaat gcaggacatt gctacgcagt ataaaaaaga aaaaggcgtg
    13501 gatgtggttt cttctttcgc ttcgtcatct actctggccc gtcagattga agcgggagcg
    13561 cctgcggatc tgtttatctc tgccgatcag aaatggatgg attatgcggt tgataaaaaa
    13621 gcgatcgata cggcttcgcg ccagacactg ctcggcaata gcctggtcgt tgtagcaccg
    13681 aaagccagcg agcagaaaga tttcaccatc gacagcaaaa ccaactggac ttcgctgctg
    13741 aatggcggtc gcctcgcggt tggcgatccg gaacatgtcc ctgctggcat ttatgcgaaa
    13801 gaagcactgc aaaaactggg cgcatgggat acgctctccc cgaaactggc tccggcggaa
    13861 gatgttcgtg gcgcgctggc gttggtcgaa cgcaatgaag cgccgttggg cattgtttac
    13921 ggttctgacg cagttgccag caaaggggta aaagtggttg ccaccttccc ggaagattca
    13981 cataaaaaag tggaatatcc ggttgctgtt gtggaagagc ataacaatgc gacagtgaaa
    14041 gcattttatg attatttgaa agggccgcag gctgcggaaa tctttaaacg ttacggattt
    14101 acaaccaagt aatgatactg accgatccag aatggcaggc agttttatta agcctgaaag
    14161 tttcttccct ggctgtgctg tttagcctgc cgtttgggat cttttttgcc tggttactgg
    14221 tgcgttgcac gtttccgggc aaagctctgc tcgacagcgt actgcatcta ccgctggtgt
    14281 taccgcccgt ggtcgtcggt tacttattat tagtttcgat gggacggcgc ggatttatcg
    14341 gtgaacgtct gtatgactgg tttggtatta ccttcgcctt tagctggcgc ggcgcggttc
    14401 tcgctgccgc cgtcatgtcg tttccgctga tggtgcgggc aattcgtctg gcgctggaag
    14461 gggttgatgt caaactcgaa caggccgcaa gaacactggg ggccgggcgc tggcgcgttt
    14521 tctttactat cacgttaccg ctgaccttac cgggaattat tgttggtacg gtactggctt
    14581 ttgctcgttc tctcggtgag tttggtgcaa ccatcacctt tgtgtcgaac attcctggtg
    14641 aaacgcgaac cattccttct gccatgtata ccctgatcca gacccccggc ggggaaagtg
    14701 gcgcggcgag actgtgcatt atttctattg cgctggcgat gatctccctg ttgatttcag
    14761 aatggctggc cagaatcagc cgtgaacggg cggggcgcta atcatgctgg aactgaattt
    14821 ttcccagacc ttgggcaacc attgcctgac cattaatgaa acgctgcccg ccaatggcat
    14881 cactgctatc tttggcgtct ccggtgccgg aaaaacttcg ctgattaacg ccatcagtgg
    14941 actgacgcgc ccgcaaaaag ggaggattgt cctcaatggt cgggtactaa atgatgccga
    15001 aaaaggtatc tgcctgacgc cagaaaagcg tcgcgttggc tatgtttttc aggatgcgcg
    15061 gctgtttccg cattacaaag tgcgtggcaa tctgcgctac ggcatggcga aaagcatggt
    15121 cgatcagttc gataagctgg tggcgctttt aggcattgaa ccgttgcttg accgtttacc
    15181 aggcagcctg tccggaggcg aaaaacagcg cgtagcgatt gggcgggctt tgctgacggc
    15241 accagaattg ctgttgctgg atgaaccgct ggcgtcactg gatattccgc gtaaacgcga
    15301 actgttgcct tatctgcaac gtctgacgcg ggaaatcaac atcccgatgt tgtatgtcag
    15361 ccattcgctg gatgagatcc tccatctggc agacagagtg atggtactgg aaaacggtca
    15421 ggtgaaagcc tttggcgcac tggaagaagt ctggggtagt agcgtgatga atccgtggct
    15481 gccgaaagag caacaaagta gcattctgaa agtgacggta cttgagcatc atccgcatta
    15541 cgcgatgact gcgctggcgc tgggcgatca gcatttgtgg gtcaataagc tggacgaacc
    15601 gctgcaagct gcgctacgta tccgcattca ggcttccgat gtttctctgg ttttacaacc
    15661 gccgcagcaa accagcattc gtaacgtatt gcgggcaaaa gttgttaata gttatgacga
    15721 caacggccag gtggaagtgg aactggaagt cggaggtaaa acgctgtggg cgcgtatcag
    15781 cccgtgggcc agggatgaac tggtgatcaa acctggcctg tggctgtacg cgcaaattaa
    15841 aagtgtgtcg ataaccgcct gattaaatca ggtggctata aatgaactgg gcaatgctgt
    15901 cggtggtgtt atcaccaatc acaatgttgg cgcgcgcttt taccgcgtca tcggcgttgc
    15961 ccatcgccac gcctgtacca gcggcctcca gcatactgat atcattaaag ttatcgccga
    16021 acgccacgac gttttccatc gaccagcctt gcgcctcaac ccatttcgtc aaacgtttac
    16081 ctttgctgtt gccgccgcgt gcaatatcaa cctgatcgtg ccaggaccat tcacactcca
    16141 gtcccagttc atgttcgaca tgcttaccaa aatgctgcaa ttgcggcagg tcatcgtgcg
    16201 tcagggcgaa cttccatacg gcgttaactt gttgcgccgt ttcagccaga gaagcgactt
    16261 gtgtgaaagt cggacgctgt tccggcggca gggtttgcgc ccagttagac gtgcgaatga
    16321 catgcccggt cgggtgctca tagaccattg catcatccac atacatcaga ccgtgaatgt
    16381 ggtgttcatt cagcatctca atgagttgca gggctttatt aacgggcatt gggtccgctt
    16441 ccagcacggt ttttgcatga taatcataca aataggtgcc attacagcaa atagcaggtg
    16501 tatccagcgc cagcgcctga taaaaaggat gaatagcgac gtgatggcga cctgtgacga
    16561 tgattaattg atagcctgct tcgcgagcgc gggccagggc ttctatcgat gaaggaagca
    16621 gggtcttttt cggggtcaat aaggtgccgt ctaagtcgag agcaatcacg cgtgtggtca
    16681 tggcgtattc cagattaagg ttaagaattt tcgtctgcgc gaatggtaca ccgataccac
    16741 tatcggacaa aattctgcgt tttaattcag cattcaccgc caaaagcgac taattttagc
    16801 tgttacagtc agttgctaaa tgcaaaggag cattcatgaa gcaaacagtt tatatcgcca
    16861 gccctgagag ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga
    16921 cacaggttgt cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt
    16981 atctctatgt tggtgttcgc cctgagtttc gcgtcctggc gtatagtatc gccccggacg
    17041 atggcgcact gacctttgcc gcagagtctg cgctgccggg tagtccgacg catatttcca
    17101 ccgatcacca ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa
    17161 cgcgtctgga agatggcctg ccagtgggcg tcgtcgatgt ggtcgagggg ctggacggtt
    17221 gccattccgc caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg
    17281 atcgcatttg cctgtttacg gtcagcgatg atggtcatct cgtggcgcag gaccctgcgg
    17341 aagtgaccac cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat
    17401 atgcgtattg cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc
    17461 acggtaatat cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc
    17521 gttgggcggc tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta
    17581 ccgccagcct gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag
    17641 gcttccagcc aacggaaacc cagccgcgcg gcttcaatgt tgatcacagc ggcaagtatc
    17701 tgattgccgc cgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg
    17761 gactactgca tgaaaaaggc cgttatgcgg tcgggcaggg accaatgtgg gtggtggtta
    17821 acgcacacta accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacctat
    17881 ctcctgtaac gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc
    17941 agtccacaaa ttaatcagca aggcaaaaaa gcgttctgct gccggagaaa gcacggcatc
    18001 tttgcggcga ataatcccca atgtccgacg tataacgggt tccaccagtg gaataccaat
    18061 aatcgaagaa tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc
    18121 cgcctctacc agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt
    18181 ccagggcttg tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac
    18241 gccaatcatt ttgtaaccca ctaactcctg ccattctaca agctgctttt tggccaatgg
    18301 atggtcacgc cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga
    18361 tgaatttgtg acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc
    18421 catgcaatta ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt
    18481 aaatttaccg attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaacgt
    18541 caccatacca cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt
    18601 ctttattaat tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt
    18661 gcgagttgta cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg
    18721 cgttaatgca ggttgcgtaa tattgagtaa tttagcagca ttgttaaatg aactggactc
    18781 tgccagtatg acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa
    18841 tggaagcgac gattttgggt ggctggtcgt taaaaatttt aactgcattt agccaactta
    18901 aattaatgaa aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta
    18961 atgtaaccat atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata
    19021 ccctgcgtga tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta
    19081 cccgaagatc aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat
    19141 ctggaaattg acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc
    19201 cgttccagcg atccgcgtgc tgatgttgat tatctgtttg ctcaagttat cgtccatgag
    19261 caacgtgtcg ataccacgcc taactgcggc aatatgcttt ctggcgttgg ggcattcgcc
    19321 attgaaaatg gtttgattgc agcgacatca ccggtcaccc gtgtgcgtat ccgcaacgtc
    19381 aatacgggta cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag
    19441 ggtagtgcca gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg
    19501 aatgccgctg gaaccaaaac cggaaaagtt ttcccgactg ataatcagat tgattatttt
    19561 gacgatgtcc cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa
    19621 tatctgggaa aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta
    19681 gcccgcattg aatctatccg tctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt
    19741 aatatggtta tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat
    19801 gtgcgttatt ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct
    19861 atttccagta gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga
    19921 tacggcaata tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa
    19981 ggtcaggatg ccacgacgtt acgcgcatct gttattcgga cgaccagaaa aatattttcc
    20041 ggtgaagttt atcttccctg aaaaaattcg ttgtcaagat aaggataatc aataaaggac
    20101 ttctgtatga gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag
    20161 ctaattctga tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta
    20221 agcgaactgg cgtgggtgct ttttggtatt tacctggcgg ccattgtggg gctggttatc
    20281 aagcctttcc cggaacctgt cgtactgtta attgccgttg ctgcctccat ggtggtggtc
    20341 ggtaacttat ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt
    20401 actacctggc tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta
    20461 ggtaaacgta ttgcctatct gctgattggt aaaatcggta acaccacgct gggtctgggt
    20521 tacgttacgg tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt
    20581 gcgggcggta ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg
    20641 gaaaaaagtc cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa
    20701 accaccagct atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc
    20761 aacgacattc tgcacctgca aattagctgg ggtggatggg cactggccgc cggattgccg
    20821 ggcatcatta tgctgctggt caccccactg gtgatttata ccatgtatcc gccagaaatt
    20881 aagaaggtgg ataataaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa
    20941 atccgcgaaa aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt
    21001 aagtctctgg gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg
    21061 ctgggtatcg ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc
    21121 tggtacggcg gtattatcgg attaagctcc ttattatcga aagttaaatt cttcgaatgg
    21181 ttagctgaag tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc
    21241 gttattattt tcctcagcat tatcgtgcgt tatttcttcg cttccggtag tgcctatatc
    21301 gttgctatgt taccggtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta
    21361 accgcgctgg cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc
    21421 gcggcaggtc cggtcatctt tggcgtgggt tataacgata ttaaatcctg gtggttggtc
    21481 ggtgcggtac tgacgatatt aaccttcctg gtgcatatca ccctcggcgt gtggtggtgg
    21541 aatatgctga tcggctggaa catgctgtaa atattctcgt catacttcaa gttgcatgtg
    21601 ctgcgtctgc gttcgctcac cccagtcact tacttatgta agctcctggg gattcactcg
    21661 cttgtcgcct tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc
    21721 acttcacggt gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt
    21781 ttctcgccag taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag
    21841 aagaagccaa aaaaggcact attgcctggt ctattctctc ttcgcataat acgtccggaa
    21901 atatggataa acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg
    21961 gtattgtaca gaccgctaaa gcgtccggta tggaacgttt cccgctgccg tatgtgctga
    22021 ccaactgcca taactcactc tgcgccgtcg gcggcactat taacggtgat gaccatgttt
    22081 ttggtttatc ggcggcccag cgttatggcg gtatttttgt gcctccgcat attgcggtca
    22141 tccatcaata tatgcgtgag atgatggcag gcggcggcaa aatgatcctc gggtcagaca
    22201 gccacacccg ttacggtgca ttagggacaa tggcagtcgg tgagggcggc ggtgagttgg
    22261 taaaacagct gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc
    22321 tgaccggaaa acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg
    22381 cggtgttcaa aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta
    22441 gcgcgctctc taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt
    22501 taagctccgt ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg
    22561 gccaggatta ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg
    22621 ttgatttaag cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg
    22681 aaatcgacac actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg
    22741 aacgcgtggc gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc
    22801 tgaaagtgca gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg
    22861 cggcggcgaa tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcagttt
    22921 acccgtcatc acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga
    22981 ttggcgcagg cgcaatcatc agaaccgcgt tctgcggccc atgctttggc gcgggcgata
    23041 cgccaatcaa caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag
    23101 gctctaagcc agctaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg
    23161 ctgcgactgc ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca
    23221 acgtgccgga gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg
    23281 tgaaaggggc aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat
    23341 tgggtgcgct gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtga
    23401 ccaccaccga cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg
    23461 gtctggcgga gtttaccctg tcacgccgcg atcccggtta tgttggcaga agtaaagcga
    23521 ctgctgagct ggaaaatcag cgtctggcgg ggaatgtcag cgagctgaca gaggtgtttg
    23581 cgcgcattaa gcagattgct ggtcaggagc atattgatcc gctgcaaact gaaattggca
    23641 gcatggtata tgcggtgaaa ccaggcgatg gttctgcgcg tgaacaggcg gcgagctgcc
    23701 agcgtgtgat tggcggtctg gcgaatattg ccgaagagta cgcgactaaa cgctaccgtt
    23761 ctaacgtcat caactggggg atgttaccgc tgcagatggc ggaagtgcca acctttgaag
    23821 tgggggatta catttacatc cctggcatta aagcggcgct ggataatccg ggtacgacgt
    23881 ttaaaggtta tgtgatccat gaagatgcgc cggtaacgga aattacgctc tatatggaaa
    23941 gtctgactgc tgaagagcgc gagattatca aggcgggtag tttgattaac ttcaataaaa
    24001 accgtcagat gtaaaaagcg ccatgtgaat gtaggtcgca ttcggcactt attgtcggat
    24061 gcgatgcttg cgcatcttat ccgacctacg aatcgcatcg aatctgtagg ccagataagg
    24121 cattttcgca gcatccggca cttattgtcg gatgcgatgc ttgcgcatct tatccgacct
    24181 acaaatcgca tcgaaccgta ggccggataa ggcgtttacg ccgcatccgg caaatagtta
    24241 attgctctta cttcttcgcc tctgcaacca ctttactacc cacgccgcgg ttattgtatt
    24301 cccacatgcg gttgtagtta gtgtcattca gattgcgctg tatttcgtcg ttatcatcta
    24361 cgctgccggt attacccgca aacggacgat tagagatcac cgcatcggcc cacggtttag
    24421 ccgtgttaaa accttcgttg atggcgctat cccggatcac cacctgaccg ttggtattgg
    24481 catcaacatc cagcgagcgg cccagttgcg ccacaccatc accgaaagca ttgaaacggc
    24541 tgtttacggc gaggaaaccg tagtaaatgt tggacagcgt agccggtgca aacacatacg
    24601 cttcttgctg agtacgtgag ttcaccacgc ggaattcggt gttatcgaac accactgcgc
    24661 cgcgaccaga aacgatatcc acatcccctt caatgtagct gttggtcacc agcgtacgcg
    24721 gctgacgatt cgtttccaga cggttctgca caccgctgtt ggtgacaaag aaggtgttct
    24781 gacgaccgag aatgttaacg ttgttaatct gtacctggtc accatcagta cgcagtgcca
    24841 ccgccggatg gttacctgca tctacgctat cgcccagcgt gttttcgatg gtcagatttt
    24901 gcagttgcag gccattgttt tgtgaccaga agaccgcaga gcagagaaca ccgatactgt
    24961 cgctgcgttt gctctggcag ctatcgtaca tataccacgc tggtttacct ggcatatatt
    25021 tgccgcgcgg gttgacgtcg tgacgccagt cggcagggct catgccacca tcaagggaaa
    25081 gcccaatctt cacatcaatc ggtttttcac ctgtaccgta cagagtaatt ccacccggag
    25141 cggcagggac atataccgtt ccctgatact caccaggcat cacggcaata tactggcgct
    25201 tgttggtacg cttgataatt gccgcatcta ccgccgcctg aatcgtggta tgcgttacac
    25261 cttgagtgcc cgccgggccg acaacaaagt caggttgcgc aggcagggta atcggggaag
    25321 gattccacgc tgctgcacct ggtgtcaggg atgcaaaata gtgttgagca tcgaaattct
    25381 gcgcttcttt tgccgacaga atcgggcgag aagaggtacc aggcgcggtt tgatcagaag
    25441 gacgttgatc gggcggggtt gagctacagg cggtcagcgt cacgccaaaa gccaatgcca
    25501 gcgccagacg ggaaactgaa aatgtgttca caggttgctc cgggctatga aatagaaaaa
    25561 tgaatccgtt gaagcctgct tttttatact aagttggcat tataaaaaag cattgcttat
    25621 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgatttcaat
    25681 tttgtcccac tccctgcctc tgtcatcacg atactgtgat gccatggtgt ccgacttatg
    25741 cccgagaaga tgttgagcaa acttatcgct tatctgcttc tcatagagtc ttgcagacaa
    25801 actgcgcaac tcgtgaaagg tcggcggatc cccttcgaag gaaagacctg atgcttttcg
    25861 tgcgcgcata aaataccttg atactgtgcc ggatgaaagc ggttcacgat gagtagatgc
    25921 aattatggtt tctccgccaa gaatctcttt gcatttatca agtgtttcct tcattgatat
    25981 cccgagagca tcaacatgca atgttgtagg gatggcaatt tttacgcctg ttttgctttg
    26041 ctcgacataa agatatccat ctacgatatc agaccacttc atttcgcata aatcaccaac
    26101 tcgctgcccg gtaacaacag ccagctccat tgcaagtctt agccaacatg gtgatgattc
    26161 tgctgcttga taaattttca ggtattcgtc agccgtaagt cttgatctcc ttacctctga
    26221 ttttgctgcg cgagtggcag cgaccgggtt tgttgttata tggccttcag ctattgcctc
    26281 tcggaatgca tcgctcagtg ttgatctgat taacttggct gacgccgcct tgccctcgtc
    26341 tatgtatcca ctgagcattg ccgcaatttc ttttgtggtg atgtcttcaa gtggagcatc
    26401 aggcagcccc ctccttattg ctttaatttt gctcatgtaa tttatgagag tcttctgctt
    26461 gattcctctg ctggcgagga ttttttcgta gcgatcaagc catgaatgta acgtaacaga
    26521 attatcactg ttgattctcg ctgtcagagg cttgtgtttg tgtcctgaaa ataactcaat
    26581 gttggcctgt attgcttcag tgattgctat cctcctgtct cggcctaatc caaactcttt
    26641 acccgtcctt gggtccctgt agcagtaata tccattgttt cttatataaa gattaggggg
    26701 taaatcccgg cgctcatgac ttcgccttct tcccatttct gatcctcttc aaaaggctac
//
LOCUS       DAZFKM010000063        26725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFKM010000063 DAZFKM010000000
VERSION     DAZFKM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..1695
                     /gene="rtcR"
                     /locus_tag="ACHKAY_002674"
     CDS             97..1695
                     /gene="rtcR"
                     /locus_tag="ACHKAY_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO5701333.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1677..2435)
                     /locus_tag="ACHKAY_002675"
     CDS             complement(1677..2435)
                     /locus_tag="ACHKAY_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO5701334.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2452..3282)
                     /gene="glpG"
                     /locus_tag="ACHKAY_002676"
     CDS             complement(2452..3282)
                     /gene="glpG"
                     /locus_tag="ACHKAY_002676"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO5701335.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3327..3653)
                     /gene="glpE"
                     /locus_tag="ACHKAY_002677"
     CDS             complement(3327..3653)
                     /gene="glpE"
                     /locus_tag="ACHKAY_002677"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO5701336.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3843..5348
                     /gene="glpD"
                     /locus_tag="ACHKAY_002678"
     CDS             3843..5348
                     /gene="glpD"
                     /locus_tag="ACHKAY_002678"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO5701337.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5400..6005)
                     /locus_tag="ACHKAY_002679"
     CDS             complement(5400..6005)
                     /locus_tag="ACHKAY_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701338.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6008..6775)
                     /locus_tag="ACHKAY_002680"
     CDS             complement(6008..6775)
                     /locus_tag="ACHKAY_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701339.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6778..7488)
                     /locus_tag="ACHKAY_002681"
     CDS             complement(6778..7488)
                     /locus_tag="ACHKAY_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701340.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7504..9012)
                     /locus_tag="ACHKAY_002682"
     CDS             complement(7504..9012)
                     /locus_tag="ACHKAY_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO5701341.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9138..11585)
                     /gene="glgP"
                     /locus_tag="ACHKAY_002683"
     CDS             complement(9138..11585)
                     /gene="glgP"
                     /locus_tag="ACHKAY_002683"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO5701342.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11604..13037)
                     /gene="glgA"
                     /locus_tag="ACHKAY_002684"
     CDS             complement(11604..13037)
                     /gene="glgA"
                     /locus_tag="ACHKAY_002684"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO5701343.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13037..14332)
                     /gene="glgC"
                     /locus_tag="ACHKAY_002685"
     CDS             complement(13037..14332)
                     /gene="glgC"
                     /locus_tag="ACHKAY_002685"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO5701344.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14350..16323)
                     /gene="glgX"
                     /locus_tag="ACHKAY_002686"
     CDS             complement(14350..16323)
                     /gene="glgX"
                     /locus_tag="ACHKAY_002686"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO5701345.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16320..18506)
                     /gene="glgB"
                     /locus_tag="ACHKAY_002687"
     CDS             complement(16320..18506)
                     /gene="glgB"
                     /locus_tag="ACHKAY_002687"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO5701346.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18779..19882)
                     /gene="asd"
                     /locus_tag="ACHKAY_002688"
     CDS             complement(18779..19882)
                     /gene="asd"
                     /locus_tag="ACHKAY_002688"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO5701347.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20074..20667
                     /gene="yhgN"
                     /locus_tag="ACHKAY_002689"
     CDS             20074..20667
                     /gene="yhgN"
                     /locus_tag="ACHKAY_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO5701348.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20724..22064)
                     /gene="gntU"
                     /locus_tag="ACHKAY_002690"
     CDS             complement(20724..22064)
                     /gene="gntU"
                     /locus_tag="ACHKAY_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO5701349.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22068..22595)
                     /gene="gntK"
                     /locus_tag="ACHKAY_002691"
     CDS             complement(22068..22595)
                     /gene="gntK"
                     /locus_tag="ACHKAY_002691"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO5701350.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22734..23729)
                     /gene="gntR"
                     /locus_tag="ACHKAY_002692"
     CDS             complement(22734..23729)
                     /gene="gntR"
                     /locus_tag="ACHKAY_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO5701351.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23953..24648)
                     /gene="yhhW"
                     /locus_tag="ACHKAY_002693"
     CDS             complement(23953..24648)
                     /gene="yhhW"
                     /locus_tag="ACHKAY_002693"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO5701352.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24771..25808)
                     /gene="yhhX"
                     /locus_tag="ACHKAY_002694"
     CDS             complement(24771..25808)
                     /gene="yhhX"
                     /locus_tag="ACHKAY_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO5701353.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26141..26629
                     /gene="yhhY"
                     /locus_tag="ACHKAY_002695"
     CDS             26141..26629
                     /gene="yhhY"
                     /locus_tag="ACHKAY_002695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5701354.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6669 a         7152 c         6661 g         6243 t
ORIGIN      
        1 ttttaaatag gttaaatctt tacgtccgta aacggagatt ttccacaaaa ccaattcacc
       61 gataatgaaa tatcgtcttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
      121 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc gaaatggcgt
      181 ccgacgcttt gtttatgcca gcaagaatcg ttagtcatcg atcggctgga attgttgcac
      241 gacacccgct cgcgctcgct attcgaaacg cttaaacgcg atatcgccag cgtttcgcca
      301 gaaacacaag tcgtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
      361 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
      421 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
      481 gcgcgttacc tgcccgcccg gctgatacaa tcttcacctc cgcgcaaaaa agaacagccg
      541 cgcggcgcag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
      601 agccgctttg ccgaggaacg ccagcaaacg cttgattttc ttaagtccgg cattgccacg
      661 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat caaatcccgc
      721 gcgccgattc tgcttaacgg tccaaccggc gcgggcaagt catttctggc gcgacgcatc
      781 ttcgagttaa aacaggcgcg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
      841 accctgcgcg gcgataccgc catgtcgacg ctgtttggtc atgtaaaagg cgcgtttacc
      901 ggggcgcggg aatctcgtga aggtttatta cgcagcgcca acggcggaat gttgtttctt
      961 gatgagattg gcgaactggg cgcagacgaa caggcaatgc tgctaaaagc cattgaagag
     1021 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
     1081 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aatttcgcga agatctgtac
     1141 acgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
     1201 gaaccgaacc tggattatga agtggagcgc cacgcctcac tcactggcga cagcgtgcgt
     1261 tttaacaccg aagcgcggcg cgcctggttg gcttttgcga cctctcccca ggcaacatgg
     1321 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
     1381 ggacgcatca ctctggacgt ggttgaagat gagataaacc gtctgcgcta taactggcag
     1441 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgatctc
     1501 ttcgaccgta tgcaactgga acacgttatc gctatctgcc gccaggcaaa gtcgctttcc
     1561 gcagccggac gccagctttt tgacgtttcg cgccagggca aagccagcgt caatgacgcg
     1621 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
     1681 cacagctcca gttgaatatg gtggtccgtc agcacctgca tcacgcttac tggcggcggg
     1741 gcgtcggtgt agacggcatc taccatgctg atgctgccca tattgaccat cgcgttacgg
     1801 ccaaatttcg agtgatcgac aaccagcata acgtggcgcg agttctcaat aatggcgcgt
     1861 ttggtgcgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctatc gatgccgctt
     1921 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
     1981 atgatcccgc catcgcggct gcgtaattcg ccaccggcga gaataatgcg gaaatcttct
     2041 tttaccatca acgtgttagc aacattgaga ttgttggtga caatacgcaa attgctgtga
     2101 ttaagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagtgtcgag
     2161 ccattgggga tttgctccgc cactttgcgg gcaatcctct ctttttcttc ggtctgggtg
     2221 gctttacgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc accgccatgg
     2281 tgacgcagga tcaggttttg ctccgccagc tcattgaggt cgcggcgaat agtctgcggg
     2341 ctgacggaga aatgctctac cagctcttca gtactgacat aaccctgctg tttaaccagt
     2401 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
     2461 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta acccggcgat
     2521 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
     2581 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcgcc
     2641 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
     2701 cccgctgaat ttttgctgca catagccgct taacagggcg ctaatgagag taataacaat
     2761 tagcttaccg ctaccgaggc gtttttccac cgcaccgccg agataccacc accagagcag
     2821 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
     2881 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatcgcc
     2941 gagaatttgc atggcgataa acaccaccac gcaggcgatc atcaccaccc aggttaccgg
     3001 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca gaccactgcc
     3061 ggtatggccc gcctgccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
     3121 acgcgccagc tccgcccgca cgcgctcggc ctgggactca tccgccagcc agacatcgct
     3181 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatataat caacaaacgc
     3241 ctgcgccacg cgggggttag caaaagaggt aatcatcaac atcgttgctg tcgcttattc
     3301 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactga
     3361 cgctgccagg cttcaaaacc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
     3421 agatactgcg ccgcgccttt gctgctattg ccgtgataac acatcaccat caccggagtg
     3481 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
     3541 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
     3601 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaacagatc catgcgtctc
     3661 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
     3721 acgccataat gttatacata tcactctaaa atgttttttc aatgttacct aaagcgcgat
     3781 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
     3841 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
     3901 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgtg
     3961 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
     4021 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
     4081 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
     4141 tgattcggat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
     4201 caaccggttt gcgttttggc gcaaactcag tgttaaaacc ggaaattaag cgcggattcg
     4261 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
     4321 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
     4381 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
     4441 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
     4501 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc
     4561 atacccagaa gcaggcctac attctgcaaa acgaagataa acgtattgtg ttcgtgatcc
     4621 cgtggatgga cgaattttcc atcatcggca ccaccgacgt agagtataaa ggcgatccga
     4681 aagcggtgaa gattgaagag agtgaaatca actatctgct gaaagtgtat aacgcgcact
     4741 ttaaaaaaca gcttagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
     4801 gtgatgatga gtccgactcg ccgcaggcta ttacccgtga ttacaccctt gatatccatg
     4861 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
     4921 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
     4981 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgcag
     5041 ctcgtctgcg ccgtcgctat ccattcctga ctgaatcgct ggcgcgtcat tacgcacgca
     5101 cttacggcag caacagcgag ctgctgctcg gcaatgcggg ggcgataagc gacctcgggg
     5161 aggatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
     5221 tccgccgcac cgacgatgcc ctttggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
     5281 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
     5341 cgtcgtaagg atgatgcccg gttgaagtgc aaccgggcta tttggattcc ttagactatt
     5401 cattgtaaat aattgaataa cggcccctga gctaaaatcg ccaaacttaa gcacgttacc
     5461 caatcgcccc acttcttcct ctggtttcta ttttttctgc accaatggca ataatatccc
     5521 catgcaaaac caagctgtgc actaatcggg ccgacaaaat cagtccaagg ctgcggccaa
     5581 aaaggggaaa aattcagggg aggccagaca aacgtcagaa agaaatttcg gatattttcc
     5641 agcaacgctg atttctctgg agaaatataa agaatgaaat tcgagagaat taaccagaaa
     5701 tagaccggcg aaaagaccag ccagcaatgc agtctgtttt ttaattcagg aggcgttctg
     5761 cgggagaatt gccaaccgat gccagccatt acactgaaaa aaagtgcata ttcaaccatt
     5821 aactcaagtt gtaattcctg aagagaaata cgcacaacca ggagcggaaa ggagaatatt
     5881 atttctgcca gaaaagttaa cgtgaatata agtacggcta agacaataac cgtcggccat
     5941 accaataatg gcacgataac aaaaccgaga attacagggg gaatataaag ccggaattta
     6001 atcattttta ttttcctggt gattcggatg atgcgtcata catcgcgcat aaacactcat
     6061 caattatcca gtcatcttcc cagacctgat attctgtcat tggttcgggc tgttttagcg
     6121 ggttctcaat cgcttgccgc gctctttctt tcagtaaagg atgattattt ctagcccagg
     6181 cattgatttg ggtggtaatg gcgttatcga tatcgtcctg gttatctctg atataatcct
     6241 cacaatatct gttcaataaa caggcaagag aaacggtcga ccaaaagccg ggaactgaat
     6301 taatggcatc cagcaatggc gcaagagcga tatcggcacg gaaaaacatt tccagcatat
     6361 actgtaattc gacagcttca tcgccggata cccaatcagt catatactgg caggcaaatc
     6421 gctgtaaaat tgcccgttca tccgcacgcc agttttcttt gttggcaagg ttgatacggc
     6481 gcagagaaaa aactacgtga atattgtcaa aatgcccctg tgcaacaaat tccagtaaac
     6541 gcggcagaaa atagcggacc tcatcgctat tttgcggatc aggccctggg ctactattcc
     6601 aggcattgat taaggaatac gggatcgctc gtaacgatgt actccgtaat gccttttgtt
     6661 cctgctgact caggcaatat tcacaacaaa ccacaaattt ctgcggcacc cgataacgac
     6721 gaaaggtgtg ataaatctgc tcaattaatg cctgcatggc ggggcagggt tgcatagtta
     6781 ccccctcgcc cgctgatcgc gtgtaaacgg ttgatagaca aaacgagcat tcgccgcgtt
     6841 caggcgaata agggcgctac gcgaaaggga aaagctgggg cgaatgcaac tattgcagtc
     6901 tcgacagctt tcctctgcgc aattttccac tgagtaatga cgcgtgtaga gtttcagttc
     6961 ggcctgctta tcgcgaaatc cgcgtaaact gttattgtta tgaaatttcc aggcatcttt
     7021 aaagacctga aagatataga catcctcaat ccaggagtca cattttttaa gcgggcgctt
     7081 tgctattatc acccgctcat ccggatttag catcgccgta aattgtacgg tgacgtcatt
     7141 tgtcgtgttc aggtcacatt gaacatctgg tacatcactg atataggggt caaatccaaa
     7201 atcagcacca caggcacaaa gacggctaat ggtggcggta tcgagggaaa atcccggcca
     7261 taccttgctg gaggttaagc caatatttaa tgtgacatca tatcgaagca atccctcaag
     7321 accgtcagca ccgacgaact tttccccttc atccagggcc tggttaataa gttgctgaac
     7381 aatgccttca tcccagtatg ccgatttgta gttggcaatc gtaatatcca cgcctgtgaa
     7441 gggatcagta ccggcaaggc ttgcaaataa tgtattaact aattccattt ttattatcca
     7501 gctttaaggt agcgtcttat acatcgggac ataacctaca tcttccacca aactctgtcc
     7561 ctgcggcgtt aaaaaccact ccaacaattt ttgtgtctct gacgtcgtat tttcccgcgt
     7621 tatcataaag gcatcgacga tatagggata tttgccgttg cgaatgtttt cggcagtcgg
     7681 tgcaataccg ttaatcgcta gcagttttat atttttatcg gcgttcattt gtgttgcgta
     7741 ataacggaag gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat
     7801 cattccctcc atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt
     7861 catgacctgg gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg
     7921 gatctcctga tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc
     7981 acgcacctgt tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa
     8041 cgcttcacgg gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt
     8101 ctgcccaccg gaaggttgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt
     8161 atacgcatcg ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat
     8221 aacgcttaaa gcataaaatg cagaagcata aattgggtac gccgccgtag caccatcgag
     8281 tcgcggccag ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt
     8341 attgagttta tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacgccggg
     8401 attgagctgt ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat
     8461 aaacaggagg gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg
     8521 ccggccgtac cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca
     8581 gtactcacct ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc
     8641 caaccaaccc gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca
     8701 gccaaccagg attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc
     8761 tcttttttct ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc
     8821 gcccagcgcc agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt
     8881 acaaacttca ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa
     8941 ttgcgtcagt atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct
     9001 gttttgcatt attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt
     9061 gccccttctg gacacttttc cgaaatgatg gcggaaaaaa acgggagcct ttggccccgt
     9121 tctatttatt ggtgaactta caatctcacc ggatcgatat gccagatatg atcggcgtac
     9181 tctttgatag tacggtcaga agagaagtag cccatattgg caatgttcag catcgctttt
     9241 gcggtccact cttcctgaag ctcgtagagt tcgtcgactt tatcctgaca atcgacatag
     9301 ctgcgataat ccgccagaac ctgatagtga tcgccgaagt tgatcagcga atcaaccaga
     9361 tcgcgatagc gacccggatc ttccggactg aatacaccgc tgccgatttg cgtcagcacc
     9421 tgatgcagct cctcatcttt ctcgtagtat tcacgcggtt tgtagccctg acgacgcagt
     9481 tcttccactt cttccgcagt gttaccaaag ataaagatat tgtcagcacc gacatgatcc
     9541 agcatctcga cgttcgcacc gtccagcgta ccgatagtca gcgcgccgtt aagcgcaaac
     9601 ttcatgttac tggtgccgga agcttccgtc cctgccagcg aaatctgttc agacagatct
     9661 gccgccggaa tgatcaactg cgccaggcta acgctgtagt tcgggatgaa cacgactttc
     9721 agcttatcgc caatctgcgg atcgttgttg atcactttcg ctacgtcatt gatcaaatga
     9781 ataatgtgct tcgccatgta ataggccgaa gccgccttac cgccaaaaat attcacgcgc
     9841 ggtacccact tcgcatccgg gtcggccttg atgcggttat agcgggtaat cacatgcaac
     9901 acattcatca attgacgttt gtattcgtga atacgtttga tttgtacatc gaacaacgcc
     9961 tttggattca ccaccacatt cagctgctgg gcgatatact ctgccagacg ctttttgttc
    10021 tccagcttcg cctgatgcac agcgtgatta accattggga aatcacagtg ttgttgcagc
    10081 tcattaagca ggctaaggtc ggtgcgccag ttacggccca ggtgttcgtc cagcacggct
    10141 gaaagcgatg ggttcgctac cgctagccag cgacgcggcg tcacaccgtt ggtgacgttg
    10201 gtgaaacgac ccgggaagat tttcgcaaag tcggcaaaca acgattgcac catcagatta
    10261 gagtgcagtt ccgatacacc gttaactttg tggctcacaa caaccgccag ccaggccata
    10321 cgcacacgac gaccgttgga ttcatcaatg atcgacgccc gtcccagcag atcggtatcg
    10381 ttcggatact gttcctgcaa ggttttcagg aaatagtcgt tgatttcaaa gatgatctgc
    10441 aggtgacgcg gcagaatttt acccagcata tcaaccggcc aggtttccag cgcctcgctc
    10501 atcagcgtat ggttagtgta ggagaagacc tgacaacaca cctcaaacgc gtcgtcccag
    10561 ctaaattggt gctcatcgat cagcagacgc atcatctcag gaatcgacag taccggatgg
    10621 gtatcattga gatgaatcgc gattttatcc gccaggttat cgtaggtttt atgcaactga
    10681 taatggcggc ttaaaatgtc ctgaatggtc gaggaaacca ggaagtattc ctgacgcagg
    10741 cgcagctcac gcccggagta ggtggagtca tccggataca gtacgcgaga tacgttctcg
    10801 gagtggtttt tatcttccac tgccgcgaag tagtcaccct ggttgaattt accgaggtta
    10861 atttcgctac tggcttgcgc actccacaaa cgcaacgtgt tggtcgcgtc ggtgtcgtaa
    10921 ccagggatta tctgatcgta agcgactccc agaatctctt cggtttcaat ccagcgcgtt
    10981 tttttacctt cctgctgaat gcgaccacca aaacggactt tatagcgcgt gttgtggcgt
    11041 ttgaattccc acgggttacc gtattccagc cagtagtctg gcgactcttt ctggctaccg
    11101 ttaacgatgt tctgcttgaa cataccgtag tcatagcgga tgccgtaacc gcgccccggc
    11161 aaccctaacg tcgccagaga atcaaggaag caagccgcca gacgtcccag gccaccgtta
    11221 ccgaggcctg ggtcattttc ttcatcaatc agctcttcga gatttaaccc catcgcttcc
    11281 agtgcgccct gtacatcttc gtaaattcct agcgacaaca tggcgttgga gagcgtacgg
    11341 ccaatcaaaa actccatcga caggtagtaa acctgacgag tttcttgcga caactgggca
    11401 cggtttgaac gtaaccagcg ctccacgaga cgatcgcgca cagcaaataa cgttgcgttc
    11461 agccattcat gtttattggc gacgaccggg tcctttccaa tcgtaaacat cagcttgtaa
    11521 gcgatagagt gcttaagagc ttctacgcta agcgtgggcg atgaatatgt aaacggagca
    11581 ttcatatagg cgtttcctga aaactatttc aagcgatagt aaagctcacg gtacgacttc
    11641 gccgcgacct gccagctaaa atccattgcc atagcctgac gttgcacaaa ccgccacagt
    11701 gaaggacggg accacagtac aaaagcacgt cgaatagccc gtaacagcga ccaggcatta
    11761 ctatcttcaa agacaaaccc actggcgacg ccatctgcaa ggttctcaag agaacagtca
    11821 gaaaccgtat cagcaagccc accggtgcgc cgcactaacg gcagcgtacc gtacttcaat
    11881 ccataaagtt gcgttaagcc gcacggttca aaacggctgg gcaccagaat gacgtccgcg
    11941 ccgcccataa tgcgatgcga aaatgcttcg tgatagccaa tctgaacgcc cacctggccg
    12001 gggtattccg ctgccgccgc aaggaaacct tcctgcagca ccggatcgcc cgcgccgagt
    12061 agcgccagct gcccgccctg ctccagaagg cccggtaagg cttccagcac caggtcgaga
    12121 cctttctggc tggtcagacg gctcaccact gcaaaaagcg gcactttatc atcaacctta
    12181 agccccattg cgatttgtaa ctggcgctta ttttccgctt tatcttccaa cgtatcgcgg
    12241 gtgtagcgcg aggccaacag taagtccgtc tctggactcc agattttctc gtccacgccg
    12301 ttcagtacgc cggaaagacg cccttcacgg tgacgctgtt gcaacagacc ttccataccg
    12361 taggcaaact gcggttcggt gatctcgcga gcgtaggttg gactaaccgc cgtaatgtga
    12421 tcggcatagt acagaccggc cttcaggaaa gagatttgtc cgttgaattc cagcccatga
    12481 atattaaaga atgaccatgg caattggatg tcattcatat gatgtgcata aaacatgcct
    12541 tgataggcca ggttgtgcac agtaaacacc gacttcgccg gacgcccgcg cgccgccaga
    12601 tacgcaggcg caaggcctgc atgccagtcg tgcgcatgca ccacatcagg acgccagaat
    12661 gggtcaagcc cgctggccat ttctgcccca acccacccca gcagcgcaaa acgcaatacg
    12721 ttgtcggtat aggcaaataa gttggtatcg tgatacgggc ttcccggacg atcatagaga
    12781 tgcggcgcgt caatcaggta tatgccaacc ccgttgtaat gaccgaacaa cagcgtgata
    12841 tgtccggcga aggtatcacg acgggatact acctgcgcat cggtaacgcc acggcgaata
    12901 tcgggaaatg caggcaacag tacgcgagcg tcaacgccgt ctgcgatttg tgctgcgggt
    12961 aatgccccaa taacatcagc cagaccgccg gttttaagca gcgggaacat ctctgaacat
    13021 acatgtaaaa cctgcattat cgctcctgtt tatgccctaa cttccgtagc atttcgcgcg
    13081 ttaccagcac gatgccttct tctgaacgat agaaacgacg tgcatcttcc tctgcgtttt
    13141 caccaatcac catgccttcc ggaataacac aagcacgatc gatgacgcag cggcgcagac
    13201 ggcacgagcg acctacccat acttccggta acaatacggc ggaatcaatg ttgcagaatg
    13261 aattcacgcg aacgcgcgag aacagaacgg actgcaccac caccgaaccg gagatcacac
    13321 agccgccgga taccagtgag ttaagggtca tcccgtggct accggagcga tcctgcacga
    13381 atttcgctgg cggtaatgat tcattgtagg tgcgaattgg ccaattgcga tcgtacatat
    13441 ccagttccgg caccacagag gccagatcga ggttcgcttt ccagtaagct tccagcgtac
    13501 ccacatcgcg ccagtacggc tcggcatccg ggtcggattg tacgcaagag agcgggaacg
    13561 ggtgcgcata ggccagaccg gcttcggtga tcttgggaat caaatctttg ccaaagtcgt
    13621 ggctggagtt ctcatcacga tcgtcttctt ccagcagttc atacagatag tcggcgtcaa
    13681 agacgtagat acccatactc gccagagatt tgctcggatc gttcggcatt gacggtgggt
    13741 tagcaggttt ttcaacgaat tcgataattt tatcgttctc atcaaccgcc ataacgccaa
    13801 atgcggaggc ttcttcaatc ggtactggca tacaagcaac ggtgcaacgt gcgccttttt
    13861 cgacgtgatc gataagcata cgcgagtagt cttgcttgta gatatggtcg cccgccagga
    13921 tcaccacgta ttccgcttta taacggcgga taatgtcgag gttttgggtg accgcatctg
    13981 cggtgccgcg ataccagttt tcccctttca ttctctgctg tgctggcagc agatcgacaa
    14041 actcgttcat ttcttcattg aagaatgacc agccgcgctg aatgtgctgc accagagtgt
    14101 gggactggta ctgggtgatc acgcccatac gacggatccc ggagttgatg cagttagaca
    14161 gcgcaaagtc gataatgcgg aacttaccgc cgaagtgtac ggccggtttt gctcgcttat
    14221 tggttaaatc cttcaggcgg gtaccacgtc ctcccgccag tatcagggca acagatttca
    14281 atggcagctg gcgcgccaac attaagtgat cgttcttctc taaactaacc atgactaact
    14341 ccttttttat catctctgga acacacacaa tccgtgtgca ggtccctgcc agacagccgt
    14401 aatcactggg ttatcctctc cagcgaatgg gggaatggcg tgccactccc cagcaggtaa
    14461 aacaatctct gttacctcaa gcgtggcgtt aattgcgatc aaaaagcgat ccgagagcag
    14521 aatttgcagc tgtttcggcc cgttttgcca ctcatccgtg cttaaaggtt gagcatatcg
    14581 atttagccaa cggacattgc cgtcgccttc ttcccaccag cgattctcca ccaaagcggg
    14641 aatgcgcttg cgcagatgga ttaacgcggc ggtaaatgcg gttaaaccac tgcttgcctg
    14701 cgaccagtcc aaccaggtta attggttatc ctgacagtag gcattgttat tgccatgctg
    14761 gctgtgacca tgttcgtcac cggccagtaa catcggcgta ccctgggaga gcaacaacgt
    14821 tgttaacagg gcgtgaatgc tgtcgcgccg ccgttcaacc aggtcaagag aaccgcctaa
    14881 cccttcttta ccatgattgt tactgtaatt gttgttggtc ccgtcgcgat tttcttctcc
    14941 gtttgcttca ttgtgtttat ggttgaagca aacgcagtcg cgaagcgtaa aaccgtcatg
    15001 cgcggtgacg agattaatcg cggcactcgg cagacgacca ttacgtttaa aaacatcgct
    15061 ggaggcagca aaacgcccgg caaacgcccc cagaggcaaa tcataatgta gccagaaacg
    15121 acgggcagca tcgcggaaat gatcgttcca ctcggcaaac agcggcggga aatttcccac
    15181 ctgataacca ccaggagcga tatcccacgg ttcagcaatt aacttcacct gcgagagcac
    15241 cgggcagttc tggatagcgg taaacaacgg cgcatcctga cggaactctg gcgtacggcc
    15301 catgactgcc gccagatcaa agcggaaacc atcgacgtgg caggtttcta cccaataacg
    15361 caggcaggcg ctggcataat ccaccaccgc cggatgactc aaattgagcg tgttgccgca
    15421 accggtccag ttgtgataat cgccgtcttc tcttatccaa taatagctac ggttatcgat
    15481 cccacgcagc gagaataacg ggccgtcgag gtccagttcc gcactatggt tgagcacgat
    15541 atcaagaatg acttcgatac ccgctttatg cagtgctttg attgcatcgc gaaactcatc
    15601 cagcgccgtt tctggcgagc aggcatacgc cggatgcagc gcaaacatcg ccaccgggtt
    15661 gtaaccccag tagttactta gccccatgcg ttgcagacgt ggttcactgg caaactgcgc
    15721 cactggcagc agttccagcg cggtaatgcc caattgtttc aaatagttga tcatcaccgg
    15781 atgcccgagg gctttataag tgccacggat ctcgaccggg atctccgggt gcaagtacgt
    15841 taatcctttg acatgggctt cataaatgat ggtgctgccc cacggcgtgc gcggcggggc
    15901 atcatcttcc cagtcatagt gatcaaccac cactacgcat ttcggcgcaa tggcggcgtt
    15961 gtcgcgatag tcaggttcat tatgaccggc gtgcagcagc gggttatctt taaactcccc
    16021 gtcaatttgc cgcgcgcaag gatcaatcaa caacttcgcc gggttaaagc gatgcccctc
    16081 ggcgggttgc caggggccat gaacgcgata accataacgc aaacccgggc gcgcatccgg
    16141 cagataaccg tgccaaatgt cgccactgtg ccctggcaag tcatagcgat gttcctggcc
    16201 attggcgtca aagacacaca gttctacccg ctcggcatga gcggagaaaa gtgtgaagtt
    16261 gacgccctga ccgtcgtaat gcgcgccgag gggagcgggt ttgccaatgg cgagttgtgt
    16321 cattctgcct cccgaaccag ccagatagtg gccagcggtg gtagcgtcag gcttagtgaa
    16381 tgctgacgac cgtggctggc aatctcatcg ctgtgtaccg tgccgccatt gcctgcatta
    16441 ctgccgtgat agtgcatgga atcggtattg aggatttcac gccatttgcc cggctggttt
    16501 atgccgaagc gataatcatg acgcggtacc ggcgtaaagt tactggcaac gatgatttcg
    16561 ttaccctctt tatcgcgacg cacaaagatc agcaccgagc gttctttgtc atccaccacc
    16621 agccattcaa agccgtacgg gtcaaaatcc agttcatgca ttgctttatg gtggcggtag
    16681 gtgaggttca gatcgcgcac cagacgctgg acaccgtggt gccagttatc gccgccttcc
    16741 aacagatgcc agtcgaggct ggcgtcatgg ttccactcgc ggccctgggc aaattcgtta
    16801 cccatgaaca gtagtttctt gcccgggaat gcccacatcc agccatagta ggcgcgcagg
    16861 ttcgcgaatt tctgccatgc gtcgcccggc atgcggtcga gaatcgattt tttaccgtgg
    16921 accacttcat catgcgacaa cggcaggacg aagttttcag tgtagttgta gagaatcccg
    16981 aaggtcagtt tatcgtgatg atactgacga taaaccgggt cgagcttcat gtagtccagg
    17041 gtgtcatgca tccagccgag gttccacttg taccagaagc ccagaccgcc catatcctgc
    17101 ggacgagaaa cgccagggaa atcggtagac tcctcagcca ttgtcaccgc accggaaacc
    17161 tgctcaccaa gaatacggtt ggtattacgc aagaattcaa tcgcttcaag attctcgcgc
    17221 ccgccaaatt cgttcgggat ccactccccc tctttacggc tgtagtcgcg ataaatcatt
    17281 gacgccaccg catcgacgcg cagcgcatca ataccaaaac gttcaatcca gtaaagcgcg
    17341 ttaccgacga ggaagttact gacttcacgg cgaccatagt tgtagatcag cgtgttccag
    17401 tcctgatgat agccttcacg cggatcgctg tgttcataca agttcgtgcc atcaaattcg
    17461 gcaagcgcaa agtcatcagt cgggaagtgg cctggcaccc agtcgagaat cacgttcaga
    17521 ccagctgcgt gtgcggcatc aatgaaataa cggaagtcgt cgcgagtacc aaaacggcgg
    17581 gttggtgcat acaggccggt tggctgataa ccccaactgc catcgaaggg atgctcgtta
    17641 atgggcagta gttcgaggtg ggtaaagccc atccatttag cataaggcac cagttgatcg
    17701 gccagctcgc ggtagctcaa ccagaaattg ttgtcggtgt ggcgacgcca ggaacccagg
    17761 tgaacttcat aaatagagat tggcgcatca aactgattcg cttttttgcg ctcttcagtc
    17821 tgtacaacct tttccggcag cccgcaaata agagacgcgg tttccgggcg catttgcgct
    17881 tcgaaggcat aagggtcgga cttcagacgc aagttgccat tggcatcaat catctcgtat
    17941 ttatagagct gaccgttatg cgccccaggg ataaacagtt cccagatgcc gctctcttta
    18001 cgcaggcgca tcgggtgacg gcgaccgtcc cagtagttga attgcccaac caccgagacc
    18061 cgacgggcgt ttggagccca gacagagaaa cgcgtacctg tgacgccatc catagtatct
    18121 gcatgcgcgc ctaaggtttc atacgggcgc aggtgagtac cttcagataa tagccaggca
    18181 tccatttcct ggattagcgg accaaaacgg taaggatcat caatcaggtt ttgctgacca
    18241 tgccagacaa cagccaactg atagcggaaa aaattcttac gtcgcggaat gacgccgcta
    18301 aagaatcccc gtgagtcgag acactccagt tttgcgagtt tgcgcccggt tttcggttca
    18361 atcacccaca catcggtagc gtcgggtaaa agggcacgga cttccagtcc cgcggtggtt
    18421 ttatgcattc ccagtacgga aaaaggatcc gcaaaatggc ctgcaattag cgcgttaatc
    18481 acgtctctat cgatacgatc ggacatgctt gtcttcctgt tttattgtgt caccccatcc
    18541 aattggattt ttgacttctg gttgtgacat ttttttgacc tgaacggcgc agcactctgt
    18601 gcatcctctc tgcgtcgtcc tcacttcagg taaggctgtg aatactcatg tattcagcca
    18661 cccttaaaga atagccaatg ctctatttaa ctcccggtaa atcatgaaac atctgcgctt
    18721 actcctgtat tacgcactaa caggggcggc atcgcgcccc agatttaatg aataaagatt
    18781 acgccagttg acgaagcatc cgacgcagcg gctccgcggc cccccacagc agctggtcgc
    18841 ccacggtaaa ggctgacagg aactctggtc ccatattcag cttacgcagg cggcctaccg
    18901 gcgtggtcag cgtgccggta acggcagctg gggttagctc acgcatagtg atttcccgat
    18961 cgttcggaac gactttcgcc cacggattgt gcgcagccag cagttcttcc acggtcggaa
    19021 tagacacatc ttttttcaat ttaatagtga atgcctggct gtggcagcgc aatgccccga
    19081 cacgcacaca taaaccatct accggaatta cggaagatgt gttgaggatc ttgttggttt
    19141 ccgcctgccc tttccactct tcgcggctct gaccgttatc gagctgtttg tcgatccacg
    19201 gaatcaggct acccgccagc ggcacgccaa agttatccac cggcagctca ccgctacggg
    19261 ttaaggttgt gactttgcgt tcgatatcga gaatagcaga ggacggggtc gcgagttcat
    19321 ctgccacatg gccatacaga tggcccatct gggttaataa ctcacgcata tgtcgcgcac
    19381 caccgccgga agcggcctgg taggttgcaa cggacaccca atcaacaaga tcattggcga
    19441 ataaaccacc caacgacatc aacatcaggc ttacggtaca gttaccgcca acaaaagtcc
    19501 tgatgccatt atttaatccg tcggtaatga cgtcctgatt gacggggtca agaatgatga
    19561 tggcgtcatc tttcatgcgc agagacgatg ctgcgtcaat ccagtaacct tgccatccgc
    19621 tttcacgaag ctttggatag atttcgttgg tataatcgcc gccctgacag gtcacaatga
    19681 tatcgagggc ctttagcgcc tccagatcaa aggcatcctg aagtgtgcca gtggttccgc
    19741 caaaagacgg cgcagcctgg ccaagctgag aagtagaaaa gaagacaggg cgaatggcgt
    19801 cgaagtcgcg ctcttcaacc atgcgttgca tgagaacgga gccgaccata ccgcgccagc
    19861 cgataaaacc aacatttttc ataagcgttt tttcctgcaa agatgtgtgc tgtataaatg
    19921 tgccggtctc ctcttggcac atctttcacc atacaaaaag cagccaaagt cgcaagtgaa
    19981 attaatcaat gatagcgaag ccatcagtaa tgcgacttat cctgctttgt tagcacgcag
    20041 aaagtccgcg gcaattatca gggaatttga gttatgaatg aaatcatttc tgcagcagtt
    20101 ttattgatcc tgattatgga tccgctcgga aacctaccta ttttcatgtc cgtactgaaa
    20161 catactgaac cgaaaagacg gcgggcaatc atggtgcgag agttgcttat tgctctcctg
    20221 gtgatgctgg tgttcctgtt tgcgggtgag aaaattctgg catttctcag cctgcgagca
    20281 gaaaccgtct ccatttctgg cggcatcatt ctgtttctaa tcgccattaa gatgattttc
    20341 cccagcgctt caggaaatag cagcgggctt ccggcaggtg aagagccatt tatcgtgccg
    20401 ttggctattc cgttagtcgc cgggccgact attctcgcca cgctgatgtt gctgtctcac
    20461 cagtacccaa atcagatggg acatctggtg attgctctgc tgctggcctg gggcggcacc
    20521 tttgtcatcc tgctacagtc ttcgctattt ttacgtctgc tgggcgagaa aggggtgaac
    20581 gcacttgaac gcctgatggg attgattctg gtgatgatgg caacccagat gttcctcgac
    20641 ggcattcgaa tgtggatgaa ggggtaacga ggagagtttt tcgaaaagaa cccggtagcg
    20701 tcacaactac cgggcgaaca aacttaactc aacagctgga acgcaatcat cccaacgatg
    20761 gcaccgacag tgccgaggat ggtttccatc atggtccagg ttttcagcgt ttcggcttcg
    20821 gtcgcgccgg taaatttacc gaacaaccag aaaccggcgt cgttaacgtg gctgacaaca
    20881 atcgaaccac cagcgataca aatcgacagc gccgccattt gcgcaccgga gtagttcagt
    20941 tgttcaataa ccggcatcac cagtcctacc gccgttaaac aggctacggt ggcagaaccc
    21001 tgaatgatgc gcactgcagc ggccagcacg aagcaggtga tggctatcgg caggcccatg
    21061 ccagttaacg cttcgcccag tgccggacct acgccagagt caaccagcac ctgtttgaac
    21121 acgccacctg caccaatcac cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa
    21181 atctccatca ctttgtcttt tggcatgccc tgacgcattg ccaggccata aatcgctacc
    21241 agacaagcaa ccagaatcgc ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg
    21301 gtagagcctt ctggcacaaa acgcgcggca atggttttca gccccaccag caccagcggc
    21361 aacaggatca gcgacaggct gaatccgaaa gatggcattt tgccttcgcc gagatgcggt
    21421 tcgctgatgt cgtcaggaat atgcaactcc acgtagcggc tgatgaaatt accccacagc
    21481 ggcccggcaa taatcattcc cggaattgcc gcacacaggc caatcaggat catccagcca
    21541 aaatcggcgt tcatctgcga tgccagcagc attggcgctg gccctggcac caggaacgcc
    21601 gccgctgccg ccacgcctgc gaataatggg attaccagct tcaccaggtt cgtaccggtg
    21661 tggcgcgcca ttgagaaagc aacgctaatc agcagaacaa tcgccacttc aaagaacagc
    21721 ggtagcgcac agaccagccc cgcaaggccg atggcataat gcgcgcggct gtgaccgaag
    21781 gatttaagca ttttgacggc aatctgatcg actgcgccgg tttcatgtaa gatcttgcca
    21841 aacatagctc ccagggcgac aaccaccgcc aggaagccga gggtgcctcc catccctttt
    21901 tccatcgtcg ctgcgatttt atcgagcggc ataccggaaa aaaggccagc ccccatggac
    21961 accaccatca aagccaggaa agcgtgcata cgcgccttca tgactaaaaa cagcagcagt
    22021 aaaacagacc ctactgctgt taaaacaagc gttaatgtag tcactactta tttgcctttt
    22081 ttaataacct caatggtgct tgccacaaca ccttccagcg gttgatcgat atccaccacc
    22141 agtacatcag tttcgtccgc acccggctcc tgcagcgttt caaactgcgt caccaacatt
    22201 tgggttttaa agaaatggcc tttgcgcgct ttcaggcggc tttcaatcac gtcaaaatcg
    22261 cctttcaggt agatgaaaga gagattcgga ttaccttcac gcagcaagtc gcgatagtgt
    22321 tttttcaatg cagaacagac gatcagtgac actttattag tgcgctgcat agcaaacgcg
    22381 gcgtcgttca gcgcctgcaa ccacggtttg cgatcgtcgt cattcagcgg ttcgccagac
    22441 gccatttttt cgatattgcg ccgtggatgg aggaaatcgc catcaagaaa cgcggcatga
    22501 agttgatgcg ccacttcact ggcgaccgca gatttgccgc tgcccgatac gcccatcaag
    22561 acgtaaatgt ggtgatcatg gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat
    22621 taaaaatgtt acgggtaact gttatcggta acattgtcca gccggacaat atcagaagca
    22681 atatccatcc gtgccttaag tgtataagtg tgagctactt caaatttgta ggcttaaata
    22741 gatccgcccg gtgacaaggt gaaacctaaa tctaacattt tcggtgtcac agattcgcca
    22801 cgaatacgcg ccagcaggcg ttcagcgcca atactgccca tccgctcacg cggcgtcagc
    22861 acgctcgcta accgcggctc catcacctgg ccaatgtcat gaccgtggaa accggcaatc
    22921 gccatatcgt caggaacctt caatcccaga cgctgacatt caaacgccgc gccgaccgcc
    22981 aggtcatcgt tggtacagaa cacgccatcc agctgcggat attcccgccg cgcctggcga
    23041 atcagttcaa taccggaaga gtaagaagaa gattgctcaa ccatcacgct atacggcacc
    23101 agacctgcat ccagcatcgc ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg
    23161 agacgcgcgc cgagataggc aatgtggcaa tgcccgcgag caataatggc ggtggtcatc
    23221 tggcgtgccg cttcaaagtt atcaaaaccg acggcgatat cgaggcacgg cgactggctg
    23281 tccatgagtt ccaccaccgg gatccccgcc acttcaatca tctttaaggt gcgcggcgtg
    23341 tgggtacgtt cggtgaggat cagtccgtcg atattccatg agagcatgga ttcgagacgt
    23401 tcttgctcca tttccggttt ataaccgtag tgcgccagca tggtctgata accgtgcgcg
    23461 tcggtgacgc tttcgattcc gcgtaatact tccgcgaaaa cctggttggt gagagaaggt
    23521 aacaggacgc caatcgcccg gctggtggcg ttagagagga tatcgggcgc acgattggga
    23581 atatagccca gttcatcaag agcggcggca atcttgccgc gtagagcgac ggaaacctgc
    23641 tccgggttgc gtaaaaaacg gctgaccgtc attttggtca cgcctacacg gtcagccaca
    23701 tcctgaagta cgggtctttt ctttttcatc gtcctgaagg tacaaaagag atagattctc
    23761 ttagtttaac acggacgcat cagaaccttc ccggggaaaa cagtgcttgt ttacaattat
    23821 tagatataca ctaaatcaat tgcatcgcat tgtgctaacg acgcggcaaa accccggaag
    23881 cgtgcatcag cacgcgaccg gagcgagcga ggaagcctgc aaaaaggcag gcttcaaaaa
    23941 tgccgtggag ctttaaaccg gcggcagatc gaacagtaac acttcgctgt cgctatccgc
    24001 atggatggag attgcctgct catcccagat tgccagacca tcgctggtcg aggctttcac
    24061 gccgttaatg gtgacattgc ctttcaccac ctggatccag acgcggcgtt cagcggcaat
    24121 ctgatgcacc gactgctcat ctttcagcaa cgcccagcgg tacagttcca tatcctgatg
    24181 cactttcaat gaaccatctc gcgcatccgg cgagagcacc agctgtttgc cctgtacggc
    24241 atcgaagcga cgctgttcat aacgcggcgt aataccgttt tcttcaggca tgatccagat
    24301 ctgatacaga tgcagacgct cggtgctgct tgggttgtac tctgagtgac gaatacccgt
    24361 acccgcactc ataatctgga actcacccgc cggaacctgc tctttattgc ccatgctgtc
    24421 ctgatgctca acagtacctt ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg
    24481 agtgccgaag ccctgccctg cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa
    24541 gcccataaag ttcggatcgt aatagttggc aaaagagaaa gtatgccagg agtccagcca
    24601 gccatgattt gcatgaccac gttcatttgc tttgcgtaag tagatcattt gtattcgccc
    24661 cctgaatgat ttcgatgggc ttagtgtgga cccgatctgc ctgggatgat agagggtgaa
    24721 aattgacccc tctgttcaaa aaaattgaac tattcgaggg gccatcaaac ttaattcgcg
    24781 agagtcactg tggagggaga ggcttgctca aatccgcgtt caaggatttc cagattggta
    24841 agaacttcag attccttgac gtaatttggc gcaccgtggg tgatggtttg atacaacgca
    24901 tcataaacgc gcccgtaatc gcccatctcc ggcttcatct cttctctgac cgtcacgccc
    24961 tcgtcattga catactccag cacaccgacc gaattatccg ctgcgaatcc cggttcgccc
    25021 ggcataatat tagccttcag gctggtttcc tgctggtcga taccgtattt aataaacgaa
    25081 cctttcttac cgtgaacgat aaatttcgga taatcgattt tcaccagatg gctggttttg
    25141 acgatggctt tcaggtcgcc ataaaacagt tgcgcttcaa aggtgtcgtc aggattggct
    25201 ttattacgca ggctgcggat gtcataagcg acgtgatccg ggcgaccgaa cagagaaata
    25261 atctggtcca tcgtatgcac accaaggcca taaaacgcgc catcctgcgg cagcccaggt
    25321 ttggtttctg ccaccgggcg gtaatagtca aaatggcttt ccacttcaac aatctctccc
    25381 aacttgccac tttcaatcgc ttttttcgct gtcaggaagc aggagtcaaa gcgacgattc
    25441 tgatacggcg tgacggtcag ccctttgctt ttcgccaacg caaacagctc tttcgcctgc
    25501 gcaagtgtcg gagtgaacgg tttttcgacc agcacatttt tcccggcttc cagcgcgcgc
    25561 ttcgcgtact cgaagtggct gtccgcgtgg gtgcagacaa caaccagctt aacatcggga
    25621 tcgtttagta cttcgtcgag atcgctggtg aaatggatat gggaataaat gggagcctgt
    25681 tcttccggct tcgcatggcg acgaaaaata tgcgcgacat gccagctatc cttgcggtta
    25741 agtacatacg gcagatggta acgggtggtg cttttgccga agccaataaa ggcgcagttg
    25801 atgaccatga tttcgtcctt tttaaggtgg ttatttacac cttagcgcaa agcggacgtg
    25861 gttcctactg gagtgcgcat aacgaacaca agcactcccg tggataaatt gagaacgaaa
    25921 gatcaaaaaa aaagccagca cccggctggc taagtaatac tggaagcaat gtgagcaatg
    25981 tcgtgctttc aggttctccg cgagggtctt cctgatcgcg agacaataat aatcattctc
    26041 attcgcactt gtccaacact ttttgcaaaa aaatgcattt gactcgcatt tgaaagtcaa
    26101 tgatgttgaa agggacattt accccaaaga ggacaaagga atgagtgaga tagtaatacg
    26161 ccacgcagaa acacgggatt acgaggccat caggcagatt cacgcccagc cggaggtgta
    26221 ttgcaacaca ctacaggtgc ctcatccttc cgatcatatg tggcaggagc gactcgccga
    26281 tcgtcccggc atcaagcaac tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac
    26341 cattgacgtg caacagcgcc cacgccgcag tcatgttgcc gattttggta tctgtgtcga
    26401 ctctcgttgg aagaaccgcg gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg
    26461 cgacaactgg ttgcgggtag atcgcattga actaaccgtg tttgtcgata acgccccggc
    26521 aattaaggtc tataaaaaat acggctttga aattgaaggg actggtaaga agtacgcatt
    26581 gcgtaatggt gaatatgtcg atgcatatta tatggcgcgg gtgaagtaag atagtgccct
    26641 ttttctgaga tggaaaaagg gtgtcattca aaatcgacat accttccttt aaggtattta
    26701 tttgcccaat acatatattg agatt
//
LOCUS       DAZFKM010000064        26225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFKM010000064 DAZFKM010000000
VERSION     DAZFKM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHKAY_002696"
     CDS             complement(<1..596)
                     /locus_tag="ACHKAY_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5701355.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKAY_002697"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHKAY_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO5701356.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKAY_002698"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHKAY_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO5701357.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKAY_002699"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHKAY_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO5701358.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKAY_002700"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHKAY_002700"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO5701359.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKAY_002701"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHKAY_002701"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO5701360.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKAY_002702"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHKAY_002702"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO5701361.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKAY_002703"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHKAY_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5701362.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKAY_002704"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHKAY_002704"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5701363.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKAY_002705"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHKAY_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO5701364.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKAY_002706"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHKAY_002706"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO5701365.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKAY_002707"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHKAY_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO5701366.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKAY_002708"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHKAY_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO5701367.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKAY_002709"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHKAY_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO5701368.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKAY_002710"
     CDS             10556..12469
                     /gene="yheS"
                     /locus_tag="ACHKAY_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5701369.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKAY_002711"
     CDS             12469..13491
                     /gene="yheT"
                     /locus_tag="ACHKAY_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5701370.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKAY_002712"
     CDS             13485..13703
                     /gene="yheU"
                     /locus_tag="ACHKAY_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO5701371.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKAY_002713"
     CDS             13757..14626
                     /gene="prkB"
                     /locus_tag="ACHKAY_002713"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO5701372.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKAY_002714"
     CDS             complement(14681..15085)
                     /gene="yhfA"
                     /locus_tag="ACHKAY_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO5701373.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15387..16019
                     /gene="crp"
                     /locus_tag="ACHKAY_002715"
     CDS             15387..16019
                     /gene="crp"
                     /locus_tag="ACHKAY_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO5701374.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKAY_002716"
     CDS             16070..18160
                     /gene="yhfK"
                     /locus_tag="ACHKAY_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO5701375.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKAY_002717"
                     /gene_synonym="dapC"
     CDS             complement(18227..19447)
                     /gene="argD"
                     /locus_tag="ACHKAY_002717"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO5701376.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKAY_002718"
     CDS             complement(19533..20096)
                     /gene="pabA"
                     /locus_tag="ACHKAY_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO5701377.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKAY_002719"
     CDS             complement(20128..20730)
                     /gene="fic"
                     /locus_tag="ACHKAY_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO5701378.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKAY_002720"
     CDS             complement(20720..20887)
                     /gene="yhfG"
                     /locus_tag="ACHKAY_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO5701379.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKAY_002721"
                     /gene_synonym="rotA"
     CDS             complement(20992..21564)
                     /gene="ppiA"
                     /locus_tag="ACHKAY_002721"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO5701380.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKAY_002722"
     CDS             21835..23016
                     /gene="tsgA"
                     /locus_tag="ACHKAY_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO5701381.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKAY_002723"
     CDS             23278..25821
                     /gene="nirB"
                     /locus_tag="ACHKAY_002723"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO5701382.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKAY_002724"
     CDS             25818..26144
                     /gene="nirD"
                     /locus_tag="ACHKAY_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO5701383.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6374 a         7109 c         6581 g         6161 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgaagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aactgcgtga ctacctcggc ggctttggtt tccagggcga
    11821 taaagtaacc gaagaaacgc gccgcttctc cggtggggaa aaagcccgcc tggtgctggc
    11881 attaattgtc tggcagcggc cgaatctgct gctgctcgac gaaccgacta accaccttga
    11941 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag tttgaaggcg cgctggttgt
    12001 cgtttcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    12061 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    12121 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aatgcgaaca gcgcccaggc
    12181 acgtaaagat cagaagcgtc gggaagctga gctgcgtgcg caaacccagc cactgcgtaa
    12241 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12301 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12361 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    12421 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12481 acgacgaccg atgccaatga attcagcagc agtgctgaat tcatccccat gcgcggcttt
    12541 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgtcaggt gaaattcacc
    12601 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatcttgc gtggagtgaa
    12661 aaccctgcac aggcgcaaca taaacctcgt ctggtggtgt ttcacgggct ggaaggcagt
    12721 ctcaacagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12781 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12841 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga atttggtcat
    12901 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12961 aaggaaggca atgatctccc ggttgatgcg gcggtgattg tctctgcgcc gtttatgctg
    13021 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    13081 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac gctgccgatt
    13141 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    13201 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13261 ctgaaccgga tcgccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13321 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13381 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13441 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13501 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13561 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13621 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatattatgc
    13681 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13741 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13801 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13861 tgcagctgag gtggaaggcg acagttttca ccgttacacc cgcccggaaa tggacatggc
    13921 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aggctaacga
    13981 cttcggcctg ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    14041 caaatatctg catacctacg acgaagccgt accgtggaat caggtaccgg ggacattcac
    14101 cccctggcaa cctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    14161 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    14221 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14281 acgagaagca gtgatggact cagtagtgcg ttcaatggaa gactatatca actacatcac
    14341 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacttcaaa
    14401 cccgttcgcg gcaaaaggta tcccgtcgct cgatgaaagc tttgtggtga tccattttcg
    14461 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14521 ccacatcaat acgttagtgg taccgggcgg caaaatgggt ctggcaatgg aattaattat
    14581 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaactct gctattacgc
    14641 cggataaaat actatccggc ttcacaacgg gatagttaag tcacgcggca accacttcat
    14701 acgagtgagt aatattcacc gctttttcca gcatcaacgc cactgaacaa tatttctcgg
    14761 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14821 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgttca gaggtcaatt
    14881 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatcgaaacc acatcgatgg
    14941 cactgcaacc acccgccgcc atcaacacca tttccatcgg acttggtgct ttatcgcctg
    15001 agttgccgtc cattaaaatc tgatgaccag aggcggattc gcccagaaaa gttaaccctt
    15061 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    15121 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    15181 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatacattga
    15241 tgtactgcat gtatgcaaag gacgtcacat taccgtgcag tacagttgat agccccttcc
    15301 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15361 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15421 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15481 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15541 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15601 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15661 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15721 acattctgat gcgtttgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15781 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15841 caaaacaacc agacgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15901 aaattggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15961 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    16021 cccgtcggag tggcgcgtta cctggtagcg cgccattttg tttcccccga tgtggcgcag
    16081 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    16141 cgtggccgtt gggttaatgc ttggcgaatt acgattcggt ctgctcttct ctctcgttcc
    16201 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16261 cattggtgcg tcgctgtttg ccacctgtag cttactgaca cagctgctac tggcaaaaga
    16321 tgttcccctg ccctttttgc tgaccggatt aacgctggta cttggcgtca ctgctgagtt
    16381 ggggccattg cacgcaaaat tgcttcccgc atcgctgctg gcagccattt ttaccctcag
    16441 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgttgg gcactctctg
    16501 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tgcgcgagtc
    16561 actaagtctg ctgtaccgtg aactggcaga ttattgtgaa gccaaataca gcctgcttac
    16621 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16681 cgatctaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataatactga
    16741 ctacaagcgg atgctgcgta ttttccagga ggcgctggat ttacaggaac atatttcggt
    16801 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16861 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16921 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16981 cgcccgccag catccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    17041 cgcccgcgtg ctgcgcaccc aaaaaccgct ctatgcacgt gacttactgg ccgataaaca
    17101 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccggcgct
    17161 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    17221 gcatctgccg aagtcgtact ggatcctgat gacggtattg ctggtgacac aaaatggcta
    17281 tggcgcaacc cgtctgagga ttgtgaatcg ctccgtggga accgtggtcg ggttaatcat
    17341 tgcgggcgtg gcgctgcact ttaaaattcc cgaaggttac accctgacgt tgatgctgat
    17401 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17461 tactattacc gcagtgtata ccctgcaact attgtggttg aacggcgagc aatacatcct
    17521 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17581 gtggccgcag tggcagagcg ggttattgcg taaaaacgcc catgacgctt tagaagccta
    17641 tcaggaagcg attcgcttga ttcttagcga ggatccgcaa cctacgccac tggcctggca
    17701 gcgaatgcgg gtaaatcagg cacataacac tctgtataac tcattgaatc aggcgatgca
    17761 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17821 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17881 acctgaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17941 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    18001 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacagc gggttattgg
    18061 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cgtcagcgac cgcatcacgg
    18121 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tgcaggctaa accatcggat
    18181 aaggcattca cgccgcatcc gacatctttt gcctgatgct tcgtatttac gccccaacca
    18241 ccttcgccac cgcgtgggcg aaacgttgca tcccttcatc gatatccgca tcttccacca
    18301 ccagcgacgg tgcaaaacgc atcacatccg gtccggcatt cagcaccatt acgccagcct
    18361 ctgcgcccgc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18421 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18481 gcagatggtc aacaaaacgc tggcgtttcg cctgaatgcc ttccagcact tcaggggtat
    18541 tgatgatatc aaacgccgcc cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18601 aaccgtgaga accaggatga aacgcagaag caatttctgc cgtggtcagc atggcgctaa
    18661 tcgggaagcc gccgcctaac gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18721 gcatgtaagc aaacaaatcg ccggtccgcc ccatcccgca ctgtacttca tcaaacacca
    18781 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18841 ccgtcacacc gccctcgccc tggatcggct caaccaccac cgcacaggtg tgatcatcca
    18901 tcaccgcttt cactgcgtgg agatcgttaa aaggaacgtg gatgatgtct gccggttttg
    18961 gcccaaagcc gtcggaatat tttggctgcc cgcccaccga gacggtaaac agcgagcgac
    19021 catgaaaagc gttatggaag gcaataattt tggttttgaa cggactatga cgcacacagg
    19081 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    19141 ccacgcgttc ggcaaacgtc gcctcaatca gtttgcgccc aagacgcagc gccggttcat
    19201 tggtgaaaac gttactgata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19261 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19321 ccttgccttg ctgatcccag attcggctgc cctgaccttt taccggaatg aactctgccg
    19381 gagcataaat cggcaggatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19441 ttgccatctc atgatcaccc tgttacgcat aaataaatgt gaaattataa tcacaaaata
    19501 tgcataaaaa atcattgaat ggcaatcaga aatcagcgat gcaggaaatt agccagcagt
    19561 tgatgtcctt gttcgctaag aatactttct ggatggaact gcacaccttc cagatcccac
    19621 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac gtcaaagcat
    19681 gctggtaatg agccaggttc caccaccagc gaatggtagc gtgtcacggt aagtggattt
    19741 gccagccccc gaaatacgcc ctcaccgtta tgtgtaatcg gcgaggtttt gccgtgcatg
    19801 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19861 cagacgccaa gaatcggcaa gcgcccggca tagtggcgaa taacgtcaag ggagatcccg
    19921 gcttcatctg gcgtacaggg gccgggtgat atgacaattt tttgtggttt aagggcgtcg
    19981 atatccgcca gcgtcaacgc atcgttgcgc ttaaccagca catccgcccc cagttcacaa
    20041 aagtactggt agaggttcca ggtaaaagaa tcgtagttat ctataagcag gatcatggcg
    20101 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    20161 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccactctgat ttgcctgatt
    20221 ccaggcctct ttttcgatac cctgccagct cagttgatac ccggcatgaa tcgccagttg
    20281 ctcgaagaag atccgctgtg ccagaccact tcccacccgg aagggatgca gcacgttgat
    20341 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20401 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20461 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccccg cccagtcgaa
    20521 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20581 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20641 ctgctgctgg tggatgttca gccggttacg catgatatca aggccaggat aaagatacgg
    20701 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagctcttca aggcgcgcta
    20761 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20821 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20881 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20941 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgaaaaatc attacggcag
    21001 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    21061 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    21121 taccgcgtaa ccgaaatcac gctgaccatg gtcaaggaag gcgttatcgg caacgttgat
    21181 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21241 cgtgttgcgc aggccgttat cggcttcatt tttgattggc gggtttggtt ttttctgctg
    21301 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21361 agtgttgtta taaaaaccgc tgttcacata atcgacaaag ttttgcacag acactggcgc
    21421 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21481 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21541 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21601 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21661 ctgaaatata tgtaacctga cgatcacttt ttattccgtg atcaaaatca cctcttaaaa
    21721 tgcaatttag caaccgattg caataaaaca tttaaacaga tcacaaaatc acctaaaatc
    21781 gcccgctcgc ggcatatccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21841 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gactggtgcg
    21901 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21961 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    22021 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    22081 atggtgctgg cggttgccgg tttgatgttc agccacagcc tggcgctgtt ctcggcggcg
    22141 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctggtaaca
    22201 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22261 atggctggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22321 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggc
    22381 tgtgagttcc cggcgctggg caaacatgcg ccaaaaacgg atgctccggt agagaaagaa
    22441 aagtggggga tcggcgtact gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22501 ttaggtttta tctcctgggt gcctgagtat gccaaaggcc tgggcatgag cctgaacgac
    22561 gcgggcacgc tggtgagtaa cttctggatg tcatacatgg tcggcatgtg ggcgttcagc
    22621 tttattcttc gcttctttga tttgcaacgc attctgaccg tactggctgg tctggctgcg
    22681 attctgatgt acgtctttaa caccggaaca ccagcacata tggcgtggtc aattctcgct
    22741 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22801 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcggaaccat cggtactatg
    22861 ttgacctttg tggttaccgg cccgattgtt gaacatagcg gtccgcaggc ggcactgctt
    22921 acagcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22981 cgtcaccgtc agcataacac cctgacctct cattaattgc tcatgccgga cggcactctc
    23041 gtcgtccggc cttttcctct cttcccccgc tacgtgcatc tatttctata aacccgttca
    23101 ttttgtccat tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    23161 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    23221 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23281 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23341 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23401 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23461 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23521 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccgggcg caccgttttt
    23581 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat caaaggttct
    23641 gatactcagg actgctttgt ctatcgcact attgaagacc tcaacgccat tgaatcctgc
    23701 gcccgtcgca gcaaacgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagccgct
    23761 ggcgcgctga aaaacttagg tattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23821 gcggaacagc ttgatcagat gggtggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23881 gtgcgcgttc acaccagcaa aaacaccctt gagattgtgc aggaaggtgt cgaagcgcgt
    23941 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    24001 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    24061 ggtatcgtca ttaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    24121 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac ctggctacaa aatggcgcag
    24181 gtcgccgttg accatattct cggtagcgaa aacgcctttg aaggtgctga ccttagcgcc
    24241 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24301 ggcgcacgta gctacgttta cctcgacgaa agtaaagaga tctacaaacg cctgattgtc
    24361 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24421 aacctgctgc aactggtgct gaatgctatc gagctgccgg aaaacccgga ttctctgatt
    24481 ctgccggcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24541 gcgcaaatct gctcctgctt cgacgtcacc aaaggtgatc tgattgctgc catcaacaaa
    24601 ggctgccaca ccgttgcggc gctgaaagct gaaaccaaag cgggtactgg ctgcggtggc
    24661 tgtatcccgc tggttactca ggtactgaac gcggaactgg cgaaacaggg catcgaagtt
    24721 aacaacaacc tgtgcgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24781 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ctacggttgt
    24841 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24901 aagccggaac atactccgct gcaggattct aacgacaact tcctcgctaa catccagaaa
    24961 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    25021 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctcccag
    25081 cgcctggcga tgtttggcgc acagaaagac gatctgccgg aaatctggcg tcagctgatt
    25141 gaagctggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    25201 gtgggtagca cctggtgccg ctacggtgtt ggcgatagcg tcggcctcgg cgtggaactg
    25261 gaaaaccgct acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25321 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac tgaaaaaggc
    25381 tggaacctgt atgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25441 gcggatatcg atcgcgaaac gctgatcaaa tatctcgacc gcttcatgat gttctacatc
    25501 cgtaccgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggcggcatc
    25561 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25621 gagatggcgc gcctgcgtga agcggtagtg tgtgagtgga ctgaaacggt caatacaccg
    25681 tccgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25741 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25801 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25861 ctgaaaccgg cgtctgcgcg ctgttaggcg acgagcaggt cgcgattttc cgcccgtatc
    25921 acagcgacca ggtgtttgcg atcagcaaca tcgatccgtt cttcgagtcc agcgtgctgt
    25981 cacgcgggct gattgcggaa caccagggcg agctgtgggt cgccagcccg ctgaaaaaac
    26041 agcgttttcg cttaagtgat ggcttgtgca tggaagacga acagttttcc gtcaaacatt
    26101 acgaagcacg agtgaaagac ggcgtggtgc agctgcgcgg ttaatgtttt aacgggaggc
    26161 gcaatgcctc ccctttttgc atggtcctgt aatgaccttc ggtataatgc aggacatttt
    26221 ttaaa
//
LOCUS       DAZFKM010000065        26137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFKM010000065 DAZFKM010000000
VERSION     DAZFKM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHKAY_002725"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHKAY_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO5701384.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHKAY_002726"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHKAY_002726"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO5701385.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHKAY_002727"
     CDS             complement(941..1408)
                     /locus_tag="ACHKAY_002727"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO5701386.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKAY_002728"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKAY_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO5701387.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKAY_002729"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKAY_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO5701388.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKAY_002730"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKAY_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO5701389.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKAY_002731"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKAY_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO5701390.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKAY_002732"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKAY_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO5701391.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKAY_002733"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKAY_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO5701392.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKAY_002734"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKAY_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO5701393.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKAY_002735"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKAY_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO5701394.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKAY_002736"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKAY_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO5701395.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKAY_002737"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKAY_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO5701396.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKAY_002738"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKAY_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO5701397.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKAY_002739"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKAY_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO5701398.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKAY_002740"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKAY_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO5701399.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKAY_002741"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKAY_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO5701400.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKAY_002742"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKAY_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO5701401.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKAY_002743"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKAY_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO5701402.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEGFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKAY_002744"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKAY_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO5701403.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKAY_002745"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKAY_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO5701404.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKAY_002746"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKAY_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO5701405.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKAY_002747"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKAY_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO5701406.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKAY_002748"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKAY_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO5701407.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHKAY_002749"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHKAY_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO5701408.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKAY_002750"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKAY_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO5701409.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKAY_002751"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKAY_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO5701410.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKAY_002752"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKAY_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO5701411.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKAY_002753"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKAY_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO5701412.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKAY_002754"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKAY_002754"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO5701413.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKAY_002755"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKAY_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO5701414.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHKAY_002756"
     CDS             15548..15916
                     /locus_tag="ACHKAY_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO5701415.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKAY_002757"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKAY_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO5701416.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKAY_002758"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKAY_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO5701417.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKAY_002759"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKAY_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO5701418.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKAY_002760"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKAY_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO5701419.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKAY_002761"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKAY_002761"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO5701420.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKAY_002762"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKAY_002762"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO5701421.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKAY_002763"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKAY_002763"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO5701422.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKAY_002764"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKAY_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO5701423.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHKAY_002765"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHKAY_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO5701424.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHKAY_002766"
     CDS             23095..23637
                     /locus_tag="ACHKAY_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO5701425.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKAY_002767"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKAY_002767"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO5701426.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKAY_002768"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKAY_002768"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO5701427.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHKAY_002769"
     CDS             25034..25291
                     /locus_tag="ACHKAY_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO5701428.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHKAY_002770"
     CDS             complement(25267..25821)
                     /locus_tag="ACHKAY_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO5701429.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6721 a         6202 c         6777 g         6437 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttctggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aaggttttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggta gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacaacgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 tgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag cagcggaggg aagcaattca gcttctggat atcctgagag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc tgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg cgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgca
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgttc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgtaaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gtcttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgccag taatacttct
    16681 tctttagttg gtgcaggtgc ggcagctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaaatcgat cccgccaatt aataagccca gaggaggcat gatgatatcg
    16921 gcaaccagtg aagagacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggttggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacactcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggtac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttatta gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 taaaacccgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgttaatc
    19501 agccccttaa gttgcgggcg cttaattgcg acagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacacac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgatt
    19801 tgttcaacgg cctgtgccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggta agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gttgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gctagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttataaag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggctttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ctagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agcccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt caagggcgcg aattttaact ttctctgcgc
    21061 gcggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgtatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttctggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacggtta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tctgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccttg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtt gggtgcgatc ctgatagcga
    23761 aacagcagtg atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
    26041 atggtcgtaa caacgaagag aaaaaatcct gaaattaagg gttgactctg aaagaggaaa
    26101 gcgtaatata cgccacctcg cgacagtgag ctgaaag
//
LOCUS       DAZFKM010000066        26058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFKM010000066 DAZFKM010000000
VERSION     DAZFKM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(131..211)
                     /gene="shoB"
                     /locus_tag="ACHKAY_002771"
     CDS             complement(131..211)
                     /gene="shoB"
                     /locus_tag="ACHKAY_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO5701430.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(631..1011)
                     /gene="acpS"
                     /locus_tag="ACHKAY_002772"
     CDS             complement(631..1011)
                     /gene="acpS"
                     /locus_tag="ACHKAY_002772"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO5701431.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(1011..1742)
                     /gene="pdxJ"
                     /locus_tag="ACHKAY_002773"
     CDS             complement(1011..1742)
                     /gene="pdxJ"
                     /locus_tag="ACHKAY_002773"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO5701432.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1754..2482)
                     /gene="recO"
                     /locus_tag="ACHKAY_002774"
     CDS             complement(1754..2482)
                     /gene="recO"
                     /locus_tag="ACHKAY_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO5701433.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2494..3399)
                     /gene="era"
                     /locus_tag="ACHKAY_002775"
     CDS             complement(2494..3399)
                     /gene="era"
                     /locus_tag="ACHKAY_002775"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO5701434.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3396..4076)
                     /gene="rnc"
                     /locus_tag="ACHKAY_002776"
     CDS             complement(3396..4076)
                     /gene="rnc"
                     /locus_tag="ACHKAY_002776"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO5701435.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4349..5323)
                     /gene="lepB"
                     /locus_tag="ACHKAY_002777"
     CDS             complement(4349..5323)
                     /gene="lepB"
                     /locus_tag="ACHKAY_002777"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO5701436.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5339..7138)
                     /gene="lepA"
                     /locus_tag="ACHKAY_002778"
     CDS             complement(5339..7138)
                     /gene="lepA"
                     /locus_tag="ACHKAY_002778"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO5701437.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7336..7815)
                     /gene="rseC"
                     /locus_tag="ACHKAY_002779"
     CDS             complement(7336..7815)
                     /gene="rseC"
                     /locus_tag="ACHKAY_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO5701438.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7812..8768)
                     /gene="rseB"
                     /locus_tag="ACHKAY_002780"
     CDS             complement(7812..8768)
                     /gene="rseB"
                     /locus_tag="ACHKAY_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO5701439.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8768..9418)
                     /gene="rseA"
                     /locus_tag="ACHKAY_002781"
     CDS             complement(8768..9418)
                     /gene="rseA"
                     /locus_tag="ACHKAY_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO5701440.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9451..10026)
                     /gene="rpoE"
                     /locus_tag="ACHKAY_002782"
     CDS             complement(9451..10026)
                     /gene="rpoE"
                     /locus_tag="ACHKAY_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO5701441.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(10023..10178)
                     /gene="rseD"
                     /locus_tag="ACHKAY_002783"
     CDS             complement(10023..10178)
                     /gene="rseD"
                     /locus_tag="ACHKAY_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO5701442.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10434..12056
                     /gene="nadB"
                     /locus_tag="ACHKAY_002784"
     CDS             10434..12056
                     /gene="nadB"
                     /locus_tag="ACHKAY_002784"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO5701443.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(12041..12778)
                     /gene="trmN"
                     /locus_tag="ACHKAY_002785"
     CDS             complement(12041..12778)
                     /gene="trmN"
                     /locus_tag="ACHKAY_002785"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO5701444.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12910..14244
                     /gene="srmB"
                     /locus_tag="ACHKAY_002786"
     CDS             12910..14244
                     /gene="srmB"
                     /locus_tag="ACHKAY_002786"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO5701445.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14453..15334)
                     /gene="yfiE"
                     /locus_tag="ACHKAY_002787"
     CDS             complement(14453..15334)
                     /gene="yfiE"
                     /locus_tag="ACHKAY_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5701446.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            15437..16024
                     /gene="eamB"
                     /locus_tag="ACHKAY_002788"
     CDS             15437..16024
                     /gene="eamB"
                     /locus_tag="ACHKAY_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO5701447.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(16080..16463)
                     /gene="grcA"
                     /locus_tag="ACHKAY_002789"
     CDS             complement(16080..16463)
                     /gene="grcA"
                     /locus_tag="ACHKAY_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO5701448.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16768..17457
                     /gene="ung"
                     /locus_tag="ACHKAY_002790"
     CDS             16768..17457
                     /gene="ung"
                     /locus_tag="ACHKAY_002790"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO5701449.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17505..18542)
                     /gene="yfiF"
                     /locus_tag="ACHKAY_002791"
     CDS             complement(17505..18542)
                     /gene="yfiF"
                     /locus_tag="ACHKAY_002791"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5701450.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18749..19168
                     /gene="trxC"
                     /locus_tag="ACHKAY_002792"
     CDS             18749..19168
                     /gene="trxC"
                     /locus_tag="ACHKAY_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO5701451.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            19237..19935
                     /gene="tapT"
                     /locus_tag="ACHKAY_002793"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             19237..19935
                     /gene="tapT"
                     /locus_tag="ACHKAY_002793"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO5701452.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            19967..22627
                     /gene="pat"
                     /locus_tag="ACHKAY_002794"
     CDS             19967..22627
                     /gene="pat"
                     /locus_tag="ACHKAY_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO5701453.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            22741..24096
                     /gene="pssA"
                     /locus_tag="ACHKAY_002795"
     CDS             22741..24096
                     /gene="pssA"
                     /locus_tag="ACHKAY_002795"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO5701454.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            24142..24465
                     /gene="yfiM"
                     /locus_tag="ACHKAY_002796"
     CDS             24142..24465
                     /gene="yfiM"
                     /locus_tag="ACHKAY_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO5701455.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(24462..25760)
                     /gene="kgtP"
                     /locus_tag="ACHKAY_002797"
     CDS             complement(24462..25760)
                     /gene="kgtP"
                     /locus_tag="ACHKAY_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO5701456.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(25877..>26058)
                     /locus_tag="ACHKAY_002798"
     CDS             complement(25877..>26058)
                     /locus_tag="ACHKAY_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701457.1"
                     /translation="QDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHLHTCSPH
                     QKFILKLLNNHFPAN"
BASE COUNT         6297 a         6866 c         6497 g         6398 t
ORIGIN      
        1 tttaattgat gacattagca taatcattca ctaagttaat ttatatagta tctgccaaga
       61 cacttattta tagttattaa aggcgcgtcc gattggttca ccggacgcac cttaagtacg
      121 tttccttgtg ttataagaac agaaggatca gctgtaaaac agcaatgatg attttgatga
      181 cccgtttaat caggtatcgg caatcagtca ttcgtttttc cttaaacaag gaatgcagcc
      241 gttcagatta gcccttacat ctccccaaaa ctgaacgtgc gagttattga gggtgcatgc
      301 tgcactccac accagagctt tgacgacacc actcgtttca atgggggaat tctgtggcat
      361 ggtgtaaagc acagcaaaat cttcaataac gaagccaatt ttaatgtact tacgaattgg
      421 cggtcacgtt aatctttcca tcaatattgc tttcttcgta aaggctcgag tttttatgct
      481 aaagattgca agttgcttgt aaaagataag tacactgatc cataatcgct gttgttgagg
      541 gtgcatgctg cacaaaatta aagttaaaaa gtaaaacccc cgttccttac cagttcgggg
      601 gttttacttt ttaaagagaa cggtattatt ttaactttca ataattaccg tggcacaagc
      661 atagtggcgt tcatctgcca gcgttacatg catatttaca acgccgagct tttccgccag
      721 ttttaatgcc tcaccccata gccgtagccg tggtttgccg agctcatcat tgaatacttc
      781 aaattgatta aacgccagac cattgcggat cccggtgcca aacgcttttg ctgcggcttc
      841 tttcacagca aaacgcttcg ccagaaaacg caccggctgg tggtgcgttt tccagattgc
      901 ccattcgtta tcgcttaata cgcggcgtgc caggcgctca ccggatcggg cgatcactgc
      961 ttctatgcga gcgatctcta cgatatccgt gcctaaacct aatattgcca ttagccacgc
     1021 gcttccagca tcagacgctt catttctgcc accgcatctt tcagtccggt catcactgca
     1081 cgaccaataa tggcatgacc gatattcagt tcatgcatct cagggatggc ggcaatagct
     1141 ttcacgttgt gataggtcag accgtgacca gcgttaactt tcagaccgag actagcggca
     1201 aaggtcgcgg ctttggcgat acgcgccagc tcttgcgcct gttcggcgtc agttttggca
     1261 tcagcatagc aaccggtgtg gatctcgata aacggtgcgc caacctctgc cgcagctttg
     1321 atctgctctt catcggcgtc gataaagaga gaaacctgga tcccggcatc tgccagacgt
     1381 ttgcaggcat cgcgcatttt gtcacgctgc cctgcgacgt ccaggccgcc ttcggttgtt
     1441 acttcctgac gcttttccgg taccagacag caaaaatgtg gcttcgtctc aacggcgatc
     1501 gccagcatct cttcggtcac cgccatctcc agattcatgc gggtatccag cgtctgacgc
     1561 aggatgcgca catcacggtc agtaatgtgg cggcgatctt cacgtaaatg cacggtaatg
     1621 ccgtccgctc ccgcctgctc agcaataaac gccgcctgca ccggatccgg gtaagcagta
     1681 ccgcgtgcgt tacgcagcgt tgcaatatgg tcaatgttga cgcctaacag taattcagcc
     1741 atgacaatcc tcatcattca taatgtgttt tcaccgttcg cttaggcata aactgccgga
     1801 acagttccct gctctttaat ggtttaccgc caagatacgg cttaagcgcc atgcgggtaa
     1861 agcgtttcgc ggcgcgtagt gtgtctgcgt caggaaattc ccgcgcgttt aacgctttta
     1921 actgccttcc ggtgaacgtt ttattgtcga taacgacgct tgcgataaac cctttttctt
     1981 cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc gctacccgca caatgggtaa
     2041 aattgacgcc atagcccaga tgcccgagca gtgccagttc aaagcggcgc agcgcgggtt
     2101 ctggcgtacc agtggcacct gcaagagact gaatgcagtg caagtaatcg aaaaagagtt
     2161 cagagaagcg cgtctcgtat tccagtacgc gggagagaag ttcgttgatg tacagaccgc
     2221 tgtaaagcgt gataccgctt aatggcagcg ccagcgagac agcttcagca ctgcgcagcg
     2281 ttttgacttc gccacgcccg ccgaaacgta gcaagagagg ggtgaaaggc tgtaatgcac
     2341 ctttcagagt agagcgttta gagcgtgcgc ctttggcaac cagacgcacg cgacccgatt
     2401 cctccgtgaa gacgtccagc atcaggctgg tttcgctcca cgggcgacta tgcaggacaa
     2461 atgcgcgctg ccagccttcc atcggagtga ctcttaaaga tcgtcaacgt aaccgagact
     2521 gcgcagtgcg cgttcgtcgt cggcccaacc ggatttcact tttacccaca gttcaaggtg
     2581 gacaggcgct tcgaacattt cctgcatgtc tttacgcgct tcaatcccga tggttttgat
     2641 cttggcccct ttgttgccaa taaccatctt cttctgccct tcacgctcaa cgagaatcaa
     2701 accattgatg tcataaccac cgcgttcgtt agagacgaaa cgttcgatct ccacggtcac
     2761 ggagtacggc agttcagcgc cgaggaaacg catcagtttt tcgcggatga tttcagacgc
     2821 cataaaacgc tgtgagcgat cggtgatgta atcttccggg aagtgatggg tcgcttcagg
     2881 gagatgctta cgcacgattg ctgcgatagt gtcgacattc agcccggttt cagcagagat
     2941 cggcacgata tcgaggaagt tcatctgact tgccaggaac tgtaagtgcg gcagcagatc
     3001 ggctttttcc tgcacgttat ccactttgtt caccgcgagg atcaccggcg ctttgccatc
     3061 gcgcagtttg ttgagcacca tttcgtcgtc cggcgtccag cgggtgcctt caacgacaaa
     3121 aatcaccagc tcaacgtcgc caatagagct gctcgccgct ttgttcatca ggcggttaat
     3181 agcgcgtttt tcttccatat gcaggccagg ggtatcaacg tagatcgcct gatacgcgcc
     3241 ttcagtatgg atccccacaa tgcggtgacg agttgtttgc gctttgcggg aggtgatgga
     3301 gatcttctgc cccagcagtt tgttcaacaa tgtggatttg ccaacgttcg gacgtccgac
     3361 gatggcaata aatccgcagt aacttttatc gatgctcatt ccagctccag ttttttcaac
     3421 gcctgttcgg cggcagcctg ctcagcctta cgacggcttg aacctgtgcc aaccaccggt
     3481 tcactcaggc cgctgacctg gcagtggata gtaaattcct gatcgtgcgc ttcgccacgt
     3541 acctggacta ccagataagt cggcagcggc agatggcgac cctgcaaata ttcttgcaag
     3601 cgcgttttcg gatctttttg tttatcgcct gggctaattt cgtccaaacg agtttgatac
     3661 cagttgagga ttaatttctc gacggtttga atatcactgt cgaggaatac accaccaatt
     3721 aatgcttcga cggtgtcggc gagaattgac tcacgacgaa atccaccgct tttaagttca
     3781 cctggcccta aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc cgccagcgta
     3841 ttgccacgga ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac acgagggaaa
     3901 cggtgataaa gcgcattggc gataacgtag ctcagaatag agtcgcctaa aaattctaaa
     3961 cgctcgttat gtttgctgct ggcactacga tgagttaatg cctgctgcaa cagttcctga
     4021 tgattaaaag tgtagcccag cttccgttga agccgattaa ttacgatggg gttcatgcga
     4081 taccaataaa taaatgcgtc aacaattcag cacacgaaac agacctgata tacatggctc
     4141 tgctaactgc ttcgctgcag tttcagttta ccggtatatg gggaccaacg ctgtttcgtg
     4201 tgccgtggca acctggaggt gccaacctta aacttcgggg gaatattcta tacacaacga
     4261 cgggggatgt cgttagccac gggagattta tctcataaat aattcacgtt gtcgccataa
     4321 cggcgacaac gtgaacgaag atgggctatt aatggatgcc gccaatgcga cttaagcgca
     4381 cacctgtcgg ccattcgcct tcttgcttat cgaaactcat ccagatagcc gttgcccgac
     4441 cgaccagatt cgcttcaggc acaaaacccc agtaacggct gtccgcgctg ttgtcgcggt
     4501 tgtcgcccat catgaagtat tgtcccggcg gaacaatcca ggttgccagt tgttgccctg
     4561 gctgctggta atacatcccc acctggtcct gcgcaatcgg cactgtcaga attcggtgcg
     4621 tcacatcacc cagtgtctct ttacgctcgg aaagacgaat tccattttct ttggtttcgt
     4681 ttttcggcac ttcaaagaat ccgctggtcg cttccccacc attacggcgt gagaaggtct
     4741 gaacgaaatc gctcggttcc acgttggagt aggtgaccgg tagcgcgttt tcacacgcct
     4801 ggccggaact gcatcccggt tgaatcgtca gctcttttga gaccggatcg taagtgactt
     4861 tatcgcccgg taaacccacc gcgcgcttga tgtaatcaag ctttggatct tccggatatt
     4921 taaagaccac gatatcgccg cgtttcggat gaccggtttc gatcagcgtt ttctggtaga
     4981 taggatcttt aatgccataa gcaaacttct ctaccagaat aaaatcaccg attaacagag
     5041 tcggcatcat cgaacctgac gggatctgga acggttcata aataaacgaa cgtacaatca
     5101 atacgatagc cagcaccgga aaaacagaag caccggtttc cagccagcca ggcttcggcg
     5161 caaccttttt cagggttgct ttatccagtg agtcaccggc agccgcctgc gccgctgcct
     5221 gacgttcccg ccgtttaggt gcgaaaaaga atttatccac gcaccataaa atgcccgtca
     5281 ccagtgtggc aatcaccaga atcagggcaa acatattcgc catgccaact cctaagggtt
     5341 atttgttgtc tttgccaacg tgcagaatgg cgaggaacgc ttcctgcggc agctcgacgt
     5401 taccgatctg cttcatgcgt ttcttacctt ctttctgctt ctgcagcagc tttttcttac
     5461 ggctgatatc gccgccataa catttagcca gtacgttttt acgtagctgt ttcacggtgg
     5521 atcgcgcaat aatgtgcgta ccaatcgctg cctgaatggc gatatcaaac tgctggcgtg
     5581 ggatcagatc tttcatcttc tccaccaact cgcgaccgcg gttttgcgaa ttatcacggt
     5641 gggtgatcag cgccaacgca tcaacacgtt caccgttgat taatacgtct acacgtacca
     5701 tgtcggacgc ctggaagcgt ttgaagttgt aatccagaga cgcataacca cgcgaggtag
     5761 atttcaggcg atcgaagaag tcgagcacca cttccgccat cgggatctcg tacgtcagcg
     5821 ccacctgatt accgtggtaa accatattgg tctgcacgcc gcgtttttct acgcacagcg
     5881 taataacgtt gccgagatat gcctgcggca gcagcatgtg acattcggca atcggctcgc
     5941 gcagttcgta gatgttattt accgctggca gcttggatgg actgtcgacg taaataactt
     6001 ctcttgacgt ggtttcaact tcatacacta cggtcggcgc agtggtgatc agatccagat
     6061 cgtattcacg ttccagacgt tcctggatga tctccatgtg cagcaggccg aggaagccgc
     6121 agcggaaacc aaagcccagc gcgctggagc tttccggctc atagaacagt gaagcatcgt
     6181 tcaggctgag tttacccagc gcgtcacgga aggcttcata gtcgtcggaa cttaccggga
     6241 acagaccggc gtatacctgc ggtttgactt tcttaaagcc aggcagcgcc ttttctgccg
     6301 gattacgcgc cagcgttaag gtatcgccga ctggagcgcc gtggatatct ttaatcgcac
     6361 ataccagcca gcctacttcg ccacatttca gttcagtgcg gtcaacctgt ttcggcgtga
     6421 agatgcccag acggtcggcg ttataggtct gcccggtact catgactttc actttgtcgc
     6481 ccttacgcag ggtgccgttt ttaatacgga taagtgaaac aacgcccagg tagttgtcga
     6541 accatgagtc gataattagt gcctgcaacg ggccttccgg atcgccttcc ggcggcggaa
     6601 tgtcgcgcac cagacgttcg agaacgtcct gcacgccaac gccggttttc gctgaacagc
     6661 gcaccgcgtc ggtggcgtcg atgccgacga tatcttcaat ttcttccgcc acgcgttcag
     6721 gatcggctgc cggcaggtca atcttgttca gtaccggcac cacttcgaga tccatttcca
     6781 tggcggtgta gcagtttgcc agggtttgcg cttctacgcc ctgcccggcg tcgaccacca
     6841 gcaatgcacc ttcacaggca gccagcgaac gggaaacttc ataggagaag tcaacgtggc
     6901 ccggggtgtc gataaagttc agctgatagg tttcgccgtc agacgctttg tagtccagcg
     6961 tcacgctttg cgctttgatg gttatgccac gctcacgctc aagatccatg gaatcgagaa
     7021 cctgcgcctc catttcacgg tcagacaggc caccgcagat ctggataata cggtcagaca
     7081 gcgtcgattt accgtggtca atgtgagcta tgatcgaaaa gttacgtata ttcttcataa
     7141 agtatgatta ttgtgcctta atgcccggtt aaccaggctt ttagaagtcg ctgttctgag
     7201 cttaacgtct gtattaatag aaacgccgca ttctacacta caacattgag gcgaggaaat
     7261 gttcataccg tatggattgt ggtatctgga aacgtcctcg catttgttat gcaaaatgca
     7321 acaaagccag tgaaatcact ggctcgcgtc ttccgaagat gtttcaaatc gcacaaggcc
     7381 aggcggcaat gccacgctta agatgatcgg ttgccattct gcccgggcag caaacttacg
     7441 cgagtagccg cgcgcaatca ggaacccgcc aataccgccg aggaccgcac cacataatgc
     7501 tgcgacgtca gaagcaaaga gtagctgaaa tagcgaagcg atgaggaata atcccaccag
     7561 cggcgacata taaaccagta atgcggagct aagcaggctg ccttcggcga tccccaattc
     7621 cactttttgc cccggcacta acggctcatc acagggtacg acaatggtat gcgtggtttg
     7681 cgggccaagt ttatttaaca cgcggctacc gcaaccggcg cgtgaagcgc agctgctgca
     7741 tgaaacttta acatcacaac tgaccagcgc ctgcccgttt tgccaggaga cgacggtagc
     7801 ccactctttg atcattgcgc tgccccgaac ttaatattct cggcaatgcg tttcgccgtt
     7861 tgcggcggca gttcaccgac aatggtgatt tcggcgttat cacgtacgct tgtactgacg
     7921 gttctgcgtc cggtgcgcaa catctgatcg gtgctcgatg gcgtagcgcg gttaacgttt
     7981 accgagaagc tgaataatcc gtcggaatag agacgtgatt cgataggcat gttgtccatc
     8041 gtcggtagcg gacgtcgact actggaaact tcgctaaaac cctgtggcaa ccaggttggc
     8101 gtccagctga atttagcttt ttcacctaca ggaacagaaa gcaacggcgg caaatttgcc
     8161 ttcgccagcg tctgcatact gctactgata tcctgattga cgttaaaagc aatcacgcga
     8221 aattgttcca gcgtttcacc atcgcgatca aggagatcaa ccctcatcgg taatttcgat
     8281 tcgatgtcca tccacacgat gtagctgtag cgtgtaccat ctcgggcaac cacgcgaatg
     8341 acttcgcaaa gacgatcagc aatacgcgtg cgcccgacgg agataaagtc gtagtaagga
     8401 gaaaggcgtt tgaaatcggt atagatgagc gatggcagag aatcaacaat gtaatcgcca
     8461 ttaagcgtga acggttcaag tcccggttca aaatagctga tttcattgcc acgctgtacc
     8521 acttcccggc gcgggccatc catttgcaac aattgtgcaa gaggacggtt atcgaggcgt
     8581 gcatgtcgat aacgcagaga ctcaacaccc tgtttattga tgctgatgaa tgacagctcg
     8641 taattcagtg actgactggc caggttcatc tgctgtaata acgccccgga cgcgggagtg
     8701 gccgaggcgt tagcagagaa taacaggcta cctgtcacta atgacatggc aaaccaaagt
     8761 tgcttcatta ctgcgattgc gttcctaaag tttgaattcc tggcacctgt acagcggctt
     8821 gctgggtttg cgcctgctca aactgaagct gttcagagtg gagtcggcgt tgcagttcgt
     8881 aatcctgcaa cattgcatta atgcgacgac gctgctcctg tacctgctgc tgttgaccat
     8941 tgtttgcggt cgcttcagaa ggtactccca ggcttaccgg gctggcttta cccatcatcg
     9001 gcagtgtatt aaataccggc gtttcgggct gctgggacgt ttcagattgt ccattatagt
     9061 gctggacgcc aacgataact gcaagcgata cgcatgctgc tacgcccatt tgggtaagct
     9121 gtgccgccca cggacgtact ttctgccaga atggcatttt ctgccattga tgcggcgcag
     9181 gctgggcttc cgggatcaat gtcgccggtt gacgtactgg ctcttcttca atggcggcca
     9241 tcacgcgtga agagatatcg aaatggagca cctcgggagt atcaccccgc attgagtcac
     9301 ggattaagtg atagctttcc caggttttct gcatttctgg gttatgagcc agttcgttaa
     9361 gcagctcact atccagcgtt tcgccatcca ttaaagcgga aagttgttct ttctgcatgc
     9421 ctaataccct tatccagtat cccgctatcg tcaacgcctg ataagcggtt gaactttgtt
     9481 atcaatagct tccctcgctc ggaagatacg tgaacgcacc gtacctaccg gacaatccat
     9541 gatagcggct atctcttcat agctcaggcc atccagctcc cgcaaggtta ttgccatgcg
     9601 caaatcttcc gggagggact caatagttcg gaaaactatc tgtctcagtt cttctgacaa
     9661 cattaagttc tcagggttcg aaatttcttt caacgcgccg ccactttcga agttttcagc
     9721 ttcaatggca tccacatcac tggaaggtgg acgacgcccc tgagcaacca ggtaattttt
     9781 cgctgtattt acagcaatcc gatacagcca tgtataaaaa gcgctatctc cccggaacga
     9841 atccagcgca cgataggctt taataaaagc ttcttgtacc acatcgggaa catcacccga
     9901 cggcacatag cgggaaacca gactcgccac tttatgctga tagcgcacta ccagtaggtt
     9961 aaaggctttc tgatctccct tctggacccg ttcaaccagg acctggtccg ttaactgctc
    10021 gctcatccga ggtaaagtct ccccaaacca aatttccacg cgctatcgaa acgccactcc
    10081 attagctgca atttgagcaa gcaaagggtt agagtgtctc gtttttgtaa agttccgtaa
    10141 cgcatctgtt tttgtttgtc atgctgtaga cggatcatta tctatcatta taagtctaca
    10201 gaatctgaac atcgcattat ctgtgtagaa atgcccattt aactgcctga agagtaaccc
    10261 aacggccttt ttatttcacc acctaatcct ccaccagcca gtaacttctc tttttctcgc
    10321 cgccctgcgt cagcgtgttt agccactgta acaaatatta aaatagcagg tgtttattcg
    10381 cacaacatga tgctatgctg accaaacggt gtttagtaaa ttaaacaaag aaaatgaata
    10441 ctctcccaga acattcatgt gacgtgttga ttatcggtag cggcgcagcc ggactttcac
    10501 tggcgctacg cctggctgac cagcatcagg tcatcgttct aagtaaaggc ccggtaacgg
    10561 aaggttcaac attttatgcc cagggcggta ttgccgccgt gtttgatgaa actgacagca
    10621 ttgactcgca tgtggaagac acattgattg ccggggctgg tatttgcgat cgccatgcag
    10681 ttgaatttgt cgccagcaat gcacgatcct gtgtgcaatg gttaattgac caaggggttt
    10741 tgtttgatac ccacgttcaa ccgaatggcg aagaaagcta ccatctgacc cgtgaaggtg
    10801 gacatagtca ccgtcgtatt cttcatgccg ccgacgccac cggtagagaa gtacaatcca
    10861 cgctggtgag caaggcgcag aaccatccga atattcgtgt gctggagcgc agcaacgcag
    10921 ttgatctgat tgtttctgac aaaattggcc tcccgggcac gcgacgggtt gttggcgcgt
    10981 gggtatggaa ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca gtggtactgg
    11041 caaccggcgg tgcgtcgaag gtttatcagt acaccaccaa tccggatatt tcttctggcg
    11101 atggcattgc tatggcgtgg cgcgcaggct gtcgggttgc caatctcgaa tttaatcagt
    11161 tccaccctac cgcactgtat cacccacagg cacgcaattt cctgttaacg gaagcactgc
    11221 gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg ttttatgccc gattttgatg
    11281 agcgcggcga actggccccg cgcgatattg tcgcccgcgc cattgaccat gaaatgaaac
    11341 gcctcggcgc agattgtatg ttcctcgaca tcagccataa gcccgccgat tttattcgcc
    11401 agcatttccc gatgatttat gaaaaattgc tcgggctggg gattgatctc acacaagaac
    11461 cggtaccgat tgtgcctgct gcacattata cctgcggtgg tgtaatggtt gatgatcatg
    11521 ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc ggcttacacg
    11581 gcgctaaccg catggcctcg aattcattgc tggagtgtct ggtctatggc tggtcggcgg
    11641 cagaagatat caccagacgt atgcctgatg cccacggcgt cagtacgtta ccgccgtggg
    11701 atgaaagccg cgttgagaac cctgacgaac gggtagtaat tcagcataac tggcacgagc
    11761 tacggctgtt tatgtgggat tacgttggca ttgtgcgcac aacgaagcgc ctggaacgcg
    11821 ccctgcggcg gataaccatg ctccaacaag aaatagacga atattacgcc catttccgcg
    11881 tctcaaataa tttgctggag ctgcgtaatc tggtacaggt tgccgagttg attgttcgct
    11941 gcgcgatgat gcgcaaagaa agccgggggc tgcattttac gctggattac ccggaactgc
    12001 tcacgcattc cggtccatcg atcctttccc ccggcaatca ttacataaac agataaaaag
    12061 cctgggtcag cgccgtatac gcttcggaat agttctggtc tggcccacga atgactaagc
    12121 gatcgctaaa gcattctccc gcctgcgggg agaatgccag cagcacccga tgcggcagtc
    12181 gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg ccagcccatg cttaatgcca
    12241 gttcagtaaa accattacca atctgctctg gcagcaccac gcagaaaaat ccctcttcgg
    12301 taatgcactc cgccgcacag gtcagcagcg atgggtgatc aagcgtagtg gtatagcgag
    12361 cctgttcccg ttgaggtgtc gcgcactcta ctccctgctg atagtaaggt gggttactga
    12421 tgattaaatc gaagcgtact gtctgctgtg tgatccactg ctgaatatcc gccgtatgga
    12481 cgttaatccg ctctgcccac ggggactggt tgatattttc ctgcgcctgc gcggcagctt
    12541 cactttccag ctcaacggca tcaattatta cgctgtcact ggttcgctgc gccagcatca
    12601 gtgccagcaa cccgctaccc gcgccgatat caaggcaacg tttcacccca gccaccggtg
    12661 cccatgcgcc cagcaaaatg ccatccgttc ccactttcat cgcacagcga tcgtgagcaa
    12721 caaaaaactg tttaaaagta aatccattac gacgaagcac ggatgtagac tgtgacatga
    12781 aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg agaacaatac ccgagaagcg
    12841 atatccatac aaacagatga agattgcagc cgtaacgtct ataatcagcg ccccacacag
    12901 aggtagaaca tgactgtaac gactttttcc gaacttgaac tcgacgaaag cctgctggaa
    12961 gccctccagg ataaaggttt cactcgcccg accgccattc aggctgccgc cattccgcct
    13021 gcgctcgatg gccgtgatgt actcggttct gcgccgacag gcaccggtaa aacggcggcg
    13081 tatctgctgc cagcgttgca gcacctgctc gatttcccgc gtaagaaatc cggtccgccg
    13141 cgtattttga tcctcacccc aactcgcgag ctggcgatgc aggtggccga tcatgcccgc
    13201 gaactggcga aacatacgca tctggatatc gccaccatca ccggcggcgt agcctatatg
    13261 aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac cggacgtctg
    13321 ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct gatcctcgac
    13381 gaagcagacc gtatgctgga tatgggcttc gctcaggata tcgaacatat tgctggcgaa
    13441 acgcgctggc gtaaacagac cctgctcttt tcggcaacgc tggaaggcga tgcgattcag
    13501 gactttgccg agcgtctgct ggaagatccg gtggaggttt ctgccaatcc gtccacccgt
    13561 gagcgcaaaa aaattcatca gtggtattac cgcgccgatg acctggaaca taaaaccgca
    13621 ttgctggtgc atctgttaaa acagccggaa gcgacccgct caattgtgtt tgtgcgtaag
    13681 cgtgaacgtg tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa caactgctat
    13741 ctcgaaggtg agatggtaca aggcaagcgt aacgaagcga tcaagcgttt gaccgaaggt
    13801 cgcgtaaacg tactggttgc aaccgatgtt gccgcgcgcg gtatcgacat tcctgacgtc
    13861 agccacgtct ttaacttcga tatgccgcgc agtggcgata cttatttgca ccgtatcgga
    13921 cgtaccgcgc gcgccggtcg taaaggcacc gcaatttcgc tggtggaagc ccatgaccat
    13981 ttgctgctgg gcaaggttgg ccgttacatt gaagagccga ttaaagctcg cgttattgat
    14041 gagttacgcc cgaaaacgcg tgcgccaagc gagaagcaga ccggcaagcc atcgaagaaa
    14101 gtactggcaa aacgcgccga gaagaaaaaa gctaaagaga aagagaaacc gcgtgtgaaa
    14161 aaacgccatc gcgacaccaa aaatattggt aagcgccgta aaccaagcgg aacgggcgtg
    14221 ccaccgcaaa cgacagaaga gtaatctcaa tgccgggttt aagcctggta ttaaaagtgc
    14281 tggcaaacgc aaaactgcct gatgcgctac gcttatcagg cctacgtggc tcatgcaata
    14341 tattgaattt gcacgatctt gtaggccgga taaggcgttc acgccgcatc cggcatgaat
    14401 aaagcgcact ttgtcgacaa gttaaatgcc gggtttaatc ccgacattat ttttatccag
    14461 ccacaaaact ctcttcaaca cactgaataa aagtgtgcat cgccgggcta accgcttttc
    14521 cagcatggtg tgcgcacatg gccgtaatgg tctgcgactg ttcgccaaaa ggcaattcaa
    14581 ttaattctcc gctctcaagc tctttttcca ccgcaaagcg gggcaagtaa ctgacaccga
    14641 tattcgccgc gacacaacgt ttgatacttt caatgcttaa cagctcaatg gtgttttcca
    14701 ccgtgatccg ccgctgacgc agcgtgctct caaatatctg ccggaagaca cattgcggtt
    14761 cgttgataat aaagctactg gcgttatgtc ttcccggctc agtaaaatcg acatctgcaa
    14821 tttgcggtga agccaccagc accagcgatt gttcacccaa ctctcgtcgg ttcagggcat
    14881 catcattccc tacgcgataa aagacgccga catcggcctc atcattcagt agcgcatcac
    14941 ggatcacgta acagttcagc gactgcaacg ataaacgcac ttttggtgcg cgttgccgaa
    15001 aacgctgcaa cacctgcggc attcggtaag agagcaacgt ttcgcccgaa acaacgcgta
    15061 gttccccgtc cggatctgac tcctttttgg cagcctcacg aagcgtatcc atcactcggg
    15121 taagttcata aatgtgcggc agcaactttt ttccttcgcg ggtaaggcac atccgtcggc
    15181 caattttctc aaataactgg actgaaaact cctgctcaag ctgctgaata tgaaaagtca
    15241 ccgtcgattg tgtacagcac aatttttgcg aagctcgcaa aaaggaaccc tcttccacca
    15301 cggttttaag cgtaataaaa cggcgcagat ccataacccc aaacctatcg aaaatatcga
    15361 atctagaata taaaaacatt cattttttta aatgttccgt gtcgggtact gtctaccaaa
    15421 acagaggaga taacaagtga caccgaccct tttaagtgct ttttggactt acaccctgat
    15481 taccgctatg acgccaggac cgaacaatat tctcgccctt agctctgcta cgtcgcatgg
    15541 atttcgtcaa agtacccgcg tgctggcagg gatgagtctg ggatttttga ttgtgatgtt
    15601 actgtgtgcg ggcatttcat tttcactggc agtgattgac ccggcagcgg tacacctttt
    15661 gagttgggcg ggggcggcat atattgtctg gctggcgtgg aaaatcgcca ccagcccaac
    15721 aaaggaagac ggacttcagg caaaaccaat cagcttttgg gccagctttg ctttgcagtt
    15781 tgtgaacgtc aaaatcattt tgtacggtgt tacggcactg tcgacgtttg ttctgccgca
    15841 aacacaggcg ctaagctggg tagttggcgt cagcgttttg ctggcgatga ttgggacgtt
    15901 tggcaatgtg tgctgggcgc tggcggggca tctgtttcag cgattgtttc gccagtatgg
    15961 tcgccagtta aatatcgtgc ttgccctgtt gctggtctat tgcgcggtac gcattttcta
    16021 ttaacgaaaa aaagcggaag aggtcgccct cttccgctta gtaacttgct acttaagcct
    16081 tacaggcttt cagtaaaggt acgagcgata acgtcgcgct gctgttccgg agtcagagag
    16141 ttaaagcgaa ctgcataacc ggatacacgg atggtcagct gcggatattt ttccggatgc
    16201 ttaactgcat cttccagagt ttcacgacgc agaacgttaa cgttcaggtg ttgaccacct
    16261 tcaacgcgaa cttctggttt cacttctact ggaacttcac ggtattcaat gtcacccagt
    16321 ttgcttactg caaccacttc atcttctgca taacctgctt ttgcaacgat gcaacgtgct
    16381 tcgccttttt cgctgtccag cagccagaaa gagttcagca gatcgtcgtt agcggcttta
    16441 gtaatctgga tacctgtaat catgtgatgc ctccccggca aaattatttg atttgttcag
    16501 cctgtcgcgg ccaattggta aaaccattgt tgcttgagtg tatatatact cctcaaccac
    16561 ccttgattct ttgatttaaa tcaataaaaa ccacacatcg attgtggtcg taaatggatt
    16621 ttattgtttt acatcaactt atgcgggtgt gaaattttac caatttacat ttttttgcac
    16681 tcgtttaagt ctaaaaaatg agcatgattt tgttctgtag aaagaagcag ttaagctagg
    16741 cggattgaag attcgcagga gagcgagatg gctaacgaat taacctggca tgacgtgctg
    16801 gctgaagaga agcaacaacc ttattttctt aatacccttc agaccgtcgc cagcgagcgg
    16861 cagtccggcg tcactatcta tccaccacaa aaagatgtct ttaacgcgtt ccgctttacc
    16921 gagttgggtg acgttaaagt ggtgattctc ggccaggatc cttatcacgg accgggacag
    16981 gcgcatggtc tggcattttc cgttcgtccc ggcattgcca ttcctccgtc attattgaat
    17041 atgtataaag agctggaaaa tactattccg ggcttcaccc gccctaatca tggctatctc
    17101 gaaagctggg cgcgtcaggg cgttctgctg ctcaatactg tgttgacggt tcgcgcaggt
    17161 caggcgcatt cccacgccag cctcggttgg gagacgttta ctgataaagt catcagcctg
    17221 attaaccagc accgcgaagg cgtggtgttt ttgttgtggg gatcacatgc gcaaaagaaa
    17281 ggggcgatta tagataagca acgccatcat gtactgaaag caccgcatcc gtcgccgctt
    17341 tcggcgcatc gtggattctt tggctgcaac cattttgtgc tggcaaatca gtggctggaa
    17401 caacgtggcg agacgccgat tgactggatg ccagtattac cggcagagag tgagtaaatt
    17461 tgcggggaaa tgccggatgg cagagttgcc acccggctga tttatcaggc tttattctga
    17521 cgccaccatt caccaagcaa aacgccggtt gcgacagaga tattcagccc ggcaacgttg
    17581 cccgtaccgt caatcttcac gcgcagatcg ttcggatcgc gtgcggcatc cggtaaccct
    17641 tcatattcct gacccagcac cagtaccatt ttcgctggca gactagtttt gaacagcggt
    17701 ttaccctgct cgctggaagt ggtcactacg gtgtaacctg cctgacggaa atcatccagc
    17761 acgttaacaa tgttgtcgcc agtaatcggc tgaacgtgct ctgcgccgcc ttctgcggta
    17821 cggatagccg cccccgactc cagcagtgcc gcatcctgca ccacgacacc tttcacgccg
    17881 aagtgcgcgc agctgcgcat catgccaccc aggttatgcg ggttagattc gttttccagt
    17941 gccagaacgc aatcctgcgc gcctgcctgg cttacccact gctgcacggt tgtaccgtta
    18001 cgctttttga tcaagaagca aacgccgccg tgatgttccg tgcctgacgc ttttgtcaat
    18061 tccgcttcat ccaccacatg gtacgctttg cggtttgctg ccatccagcg caaggcttct
    18121 ttaaaacgcg gcgttacact ctggataaac caggcgcgaa caatcgcttc cggacggctc
    18181 tggaaaagtg cctgacatgc attttcgccg tagacgcggg tttcttccgc acgctgacga
    18241 cgcaacactt ccggatcaat aaaactttta ccactgatgc caccgtgatc ggccttttcc
    18301 ggcgtctcat cacccggcgc gcgggaaacc gtgcgccacg gcgaatcttc ccacttgcgg
    18361 tcacggggct gactctgttt gtcatcgcgg gcggggcgac ggccaccgtc agcacgagat
    18421 tttcctggac gcccgccccc tttcccggta cgcgggttgt gggtacgttt atcagaatca
    18481 tcatcactgc ggacatacat cactttgacc ttgccgcttt tacctttcat ttcatcgttc
    18541 atgcttttct ccaccagcgc tgcgcgaagc gcgcagatta cccgaagtcc gcgcggttcg
    18601 ccatgatttc gtaccaaagc ctgcgactat catacctatt gaataaaaca gattgttgtc
    18661 tggaacaatg tccccgataa tatgtaacat attagaaaca taccggcgtc gttgccgata
    18721 agtctcctta ctcatcccga ggttagttat gaataccgtt tgtacccatt gtcaggccat
    18781 caatcgcatt cccgacgatc ggatcgaaga tgcggcaaaa tgcggacgct gcggtcacga
    18841 cttgtttgac ggagaggtga ttaatgcgac cggtgaaaca ctcgacaaat tgctgaagga
    18901 tgatctacct gtggtgatcg acttctgggc accgtggtgc ggcccctgcc gtaatttcgc
    18961 accaattttt gaagatgtcg cgcaagagcg tagcggtaaa gtgcgctttg tgaaagtgaa
    19021 taccgaagct gaacgtgaat tgagcagtcg ctttggaatt cgtagtatac cgacgatcat
    19081 gattttcaaa aacggtcagg ttgtcgacat gcttaatggc gcagtaccga aagcgccgtt
    19141 cgatagctgg ctgaacgaat ctctttaatc ttaccggggc gcatcttgtg ccccgttttc
    19201 tcctctgcga caatggcgtt ttttcgacgc tctcttatga ccgaaaacgg tgttctccag
    19261 ttacgcgccg agcgtattgc gcgcgcaaca cgtccttttc ttgcccgcgg taatcgcgtt
    19321 cgtcgctgcc aacgctgtct tttgccagag aaattatgtc tctgttcgac aattacccca
    19381 gcacaagcaa aaagtcgctt ctgtttgctg atgttcgaca ccgagccaat gaagcccagt
    19441 aataccgggc gtctgattgc tgatattttg cctgataccg ttgcgtttca atggtcgcgt
    19501 accgaaccct cgcaggattt gctggagttg gtgcaaaacc cggactatca gccaatggtg
    19561 gtctttcccg cttcgtatgc tgatgagcaa cgggaagtga tcttcacacc acctgccggt
    19621 aagccaccgc tgtttatcat gctcgatggt acctggccgg aagctcgcaa gatgtttcgt
    19681 aaaagtccgt atctggataa tcttcccgtc atttccgtcg atctttcccg gctttctgcc
    19741 tatcgcctgc gtgaagccca ggctgcaggc caatattgta ctgccgaggt agccatcgca
    19801 ctgttagata tggccagcga taccggggcg gcagcagggt taggcgagca ttttacccgc
    19861 tttaaaacac gctatctggc aggaaaaacg caacatctgg gtagcatcac agcagaacag
    19921 ttagaaagcg tttaaaatca ttcggtcact tctgcgggag accggtatga gtcagcgagg
    19981 actggaagca ctactgcgac caaaatcgat agcggtaatt ggcgcgtcga tgaaacccaa
    20041 tcgcgcaggt tacctgatga tgcgtaacct gctggcggga ggctttaacg gaccggtact
    20101 cccggtgacg ccagcctgga aagcggtgtt gggtgtgttg gcctggccgg atattgccag
    20161 cttgcccttt acacccgacc ttgcggtttt atgtaccaat gccagccgta atcttgctct
    20221 tctggaagag ctcggcgaga aaggctgtaa aacctgcatt attctttccg ccccggcatc
    20281 gcaacacgaa gatctccgcg cctgcgccct gcgccataac atgcgcctgc ttggaccaaa
    20341 cagtctgggt ttactggctc cctggcaagg tctgaatgcc agcttttcgc ctgtgccgat
    20401 taaacgcggc aagctggcgt ttatttcgca atcggctgcc gtctccaaca ccatcctcga
    20461 ctgggcgcaa cagcgtaaga tgggcttttc ctactttatt gcgctcggcg acagcctgga
    20521 tatcgacgtt gatgaattgc ttgactatct ggcacgcgac agtaaaacca gcgccatcct
    20581 gctctatctc gaacagttaa gcgacgcgcg acgctttgtt tcggcggccc gtagtgcctc
    20641 tcgcaacaaa ccgattctgg tgattaaaag cggacgtagc ccggcggctc aacgtctgtt
    20701 gaacaccacg gcaggaatgg atccggcgtg ggatgcggct attcagcgtg ccggtttgtt
    20761 gcgggtacag gacacccacg aactgttttc ggcggtggaa acccttagcc atatgcgccc
    20821 gctgcgtggc gaccggttga tgattatcag caacggtgct gcgcctgccg cgctggcgct
    20881 tgatgcctta tggtcacgca atggcaagct ggcaacacta agcgaagaaa cctgccagaa
    20941 actgcgcgat gcactgccag gacatgtggc aatctctaac ccgctcgatc tgcgcgatga
    21001 cgccagcagt gagcactata ttaaaacgct ggacattctg ctccacagcc aggattttga
    21061 tgcgctgatg gttattcatt cgcccagcgc cgctgctccc gcaacagaaa gcgcgcaagt
    21121 attaattgaa gcggtaaagc atcatccccg cagcaagtat gtttctctgc tgacgaactg
    21181 gtgtggcgaa cattcctcgc aagaggcacg acgattattc agcgaagccg ggctgccgac
    21241 ctaccgtacc ccggaaggaa ccatcactgc ttttatgcat atggtggagt accggcgtaa
    21301 tcagaagcaa ctacgcgaaa cgccggcgtt gcccagcaat ctgacctcca ataccgcaga
    21361 agcgcatctt ctgttgcaac aggcgattgc cgaaggagca acgtcactcg atacccatga
    21421 agttcagcct atcctgcaag catatggcat gaacacgctc cccacctgga ttgccagcga
    21481 tagcaccgaa gcggtgcata ttgccgaaca gattggttat ccggtggcgc tgaaattgcg
    21541 ttcgccggat attccacata aatcggaagt tcagggcgtc atgctctacc tgcgtacagc
    21601 caatgaagtc cagcaagcgg caaacgccat tttcgatcgc gtaaaaatgg cctggccgca
    21661 ggcacgggtc cacggcctgt tggtgcaaag tatggctaac cgtgctggtg ctcaggagtt
    21721 gcgggttgtg gttgagcacg atccggtttt cgggccgttg atcatgctgg gcgaaggcgg
    21781 tgtggagtgg cgtcctgaag atcaagccgt cgtcgcgtta ccgccgctga acatgaacct
    21841 ggcccgctat ctggttattc aggggatcaa aagtaaaaag attcgtgcac gcagtgcgct
    21901 acgcccattg gatgttgcag gcttgagcca gcttctggtg caggtttcca acttgattgt
    21961 cgattgcccg gaaattcagc gtctggatat tcatcctttg ctggcttctg gcagtgaatt
    22021 taccgcgctg gatgtcacgc tggatatctc gccgtttgaa ggcgataacg agagtcggct
    22081 ggcagtgcgc ccttatccgc atcagctgga agaatgggta gaattgaaaa acggtgaacg
    22141 ctgcttgttc cgcccgattt tgccagaaga tgagccacaa cttcagcaat tcatttcgcg
    22201 agtcaccaaa gaagatcttt attaccgcta ctttagcgag atcaacgaat ttacccatga
    22261 agatttagcc aacatgacac agatcgacta cgatcgggaa atggcgtttg tagcggtacg
    22321 acgtattgat caaacggaag agatcctcgg cgtcacgcgt gcgatttccg atcctgataa
    22381 catcgatgcc gaatttgctg tgctggttcg ctcggatctc aaagggttag gcttaggtcg
    22441 acgcttaatg gaaaagttga ttacctatac gcgagatcac ggactacaac gtctgaatgg
    22501 tattacgatg ccaaacaatc gtggcatggt ggcgctagcc cgcaagctcg ggtttaacgt
    22561 tgatatccag ctcgaagagg ggatcgttgg gcttacgcta aatcttgccc aacgcgagga
    22621 atcatgagta aggtactgga aatgttgacc acttaatcgg gactggtgtt attattgccc
    22681 gcttatgtcg tctgcattgc acagaggacc cttcaatgaa cagagaagaa atgcactgtg
    22741 atgttgtcaa aatttaagcg taataaacat caacaacacc ttgcccaact acccaagatt
    22801 tctcaatcag ttgatgatgt cgatttcttt tacgctcccg ccgacttccg ggagacgctg
    22861 ctggaaaaaa tagccagcgc gaagcagcgc atttgcattg tcgccctgta tctcgaacag
    22921 gatgacggtg gcaaaggcat tctgaacgcg ttgtatgaag ctaaaaggca gcgtccggaa
    22981 ctggatgtgc gggtgctggt cgactggcat cgtgcacaac gtggacgcat tggcgctgcg
    23041 gcatctaaca ctaacgctga ctggtactgc cgcatggcgc aggaaaatcc gggcgtagat
    23101 gttccggttt atggcgttcc aatcaatacc cgtgaagccc ttggtgttct gcactttaaa
    23161 ggctttatca tcgacgatag cgtactttat agcggtgcca gcctgaacga tgtttacctg
    23221 catcagcacg ataaatatcg ctacgaccgt tatcatctta tccgtaaccg taagatgtca
    23281 gacattatgt ttgaatgggt tacacagaat attatgaatg gccgcggcgt taatcgtctg
    23341 gatgatgtta atcggccaaa aagcccggaa atcaagaacg atattcgtct gttccgccag
    23401 gagctgcgtg atgccgctta tcatttccag ggcgatgccg acaacgatca gctttccgta
    23461 acgccgctag tggggctggg gaaatcgagt ctgttgaaca agaccatttt ccatcttatg
    23521 ccttgtgccg agcagaaact aaccatctgt acgccatact tcaacctgcc agcaatcctt
    23581 gtgcgcaata ttatccagtt gctgcgcgaa gggaaaaagg tcgaaattat tgttggtgat
    23641 aaaaccgcga acgacttcta cattccggaa gatgaacctt tcaagataat tggcgcattg
    23701 ccttatctct atgagatcaa tctgcgtcgt ttcctgagcc gtttgcagta ttacgtcaat
    23761 actgaccagc tagtggttcg gttatggaaa gatgacgaca acacctatca cctaaaaggg
    23821 atgtgggttg atgataagtg gatgttgatc accggtaata acctgaaccc gcgcgcctgg
    23881 cgtctggatc tggaaaacgc cattttgatc cacgatccac aactggagct tgcgccgcag
    23941 cgagagaaag aactggagct gatccgcgag cataccacca tcgttaagca ctatcgcgat
    24001 ctgcaaagta ttgccgatta tccggtgaag gttcgtaaac tcatccgccg tttgcgccgt
    24061 atccgcatcg accgattaat tagccgcatc ctgtaatcac aaccccgtcc tgtacggggt
    24121 ttgttttttg gaggccacgt tttgcgtatt ctttttgtct gttcgctatt gttactttct
    24181 ggatgcagcc atatggctaa cgatagctgg agcgggcagg ataaagctca acactttatc
    24241 gcctcggcga tgctttccgc cgccggaaat gaatattcac agcatcaggg gatgagccgg
    24301 gatcgcagtg ccatgtttgg attgatgttc tctgtcagtt tgggggcgtc aaaagagctt
    24361 tgggatagcc gccccgaagg gagcggctgg agctggaagg atttggcctg ggatgtcgcc
    24421 ggtgcaagca ccggctatac cgtctggcaa ctgacccgtc actaaagacg catccccttc
    24481 cctttgcgat gtagcatcaa agaaaccaga aacgccacca cggccatcaa ggtcacatac
    24541 cagaagaagg ctgtttccat tcctattgat ttcagcgaca acgctacgta ctccgccgaa
    24601 ccaccaaata tagcattagc gaccgcatat gacaggccaa cgcctaatgc gcgaacctgt
    24661 gccgggaaca tctcagcctt cagtattcca ctgattgatg tataaaaact cactatcagc
    24721 agggcacaca tcaccagacc aaaagcggca taaggcgagg aaacgttttg caatgctgag
    24781 agaataggaa cggtaaaaat ggctgccagc gaaccgaaac ataacattga ggtacggcga
    24841 ccaatcttat ccgacagcgc gccaatgagt ggttgaataa gcatgaatac aaacaatgcg
    24901 gcagtcataa tgccactcgc cacgttggca tgcattcccg cagtatttac cagatacttc
    24961 tgcatataag tggtgaaggt atagaaacaa agggagcccg cagcggtaaa accgagaacc
    25021 atgatgaatg cacggcgatt gcgccataat cctttcagag atccagcttc ttttaaagcg
    25081 cgcgtttctt gttgcgaagt ttcatctaat tgacgacgta accacaacgc cacaacagct
    25141 aacacagctc ctaacgcgaa aggaatacgc catccccact ctctgagtgc agcgtcttcc
    25201 atggtgtgtt gtaaaaccac gacaaccagt agggctagca gttgtccgcc gatcaacgtc
    25261 acatactgaa atgatgcgta aaaacctttg cgcccttcaa cggcaacttc actcatatag
    25321 gtggcgctgg tgccatattc tccgccaacg gataatccct gaaataaacg agcgagaagc
    25381 aataatgccg gagcccacgt acctatagtt tcataacctg ggaggcaggc gataaccagc
    25441 gatccgaaac acatcataca caccgataac agcatcgatt ttttgcgacc atgtttatcg
    25501 gctatgcggc caaatagcca accgcctatt gggcgcatca ggaatcccgc agcaaaaaca
    25561 cctgctgttt gtagtagttg agtcgtcgtg ttcccggaag ggaagaaaat gtgggcgaag
    25621 tagagtgaac agaacgagta gacatagaaa tcgaaccact cgaccagatt acctgaagag
    25681 gcccccacaa tcgcccaaat gcggcgacgt gtatcactac ttgtcagttt gctgtctgcc
    25741 gttacagtac tttcagccat gccattatgt ctcctgccgt aatccgatgc ttttgtcggt
    25801 cgcttttgtt tatttctttt ggtaaagaaa atgttataca tttgttgcgt atcattatgc
    25861 aaccttaacc atgaatttag ttagcaggaa aatggttatt gaggagctta aggataaatt
    25921 tctggtgagg agaacacgta tggaggtggg caagttgggg aagccgtatt cgctgctgaa
    25981 tctggcatat gtgggagtat aagacgcgca gcgtcgcatc aggcattttt ttcaccaacg
    26041 caaaaaggcc atcctgac
//
LOCUS       DAZFKM010000067        24741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFKM010000067 DAZFKM010000000
VERSION     DAZFKM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..220
                     /locus_tag="ACHKAY_002799"
     CDS             68..220
                     /locus_tag="ACHKAY_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5701458.1"
                     /translation="MDTIDLGNNASLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LAKNTS"
     gene            641..862
                     /gene="kilR"
                     /locus_tag="ACHKAY_002800"
     CDS             641..862
                     /gene="kilR"
                     /locus_tag="ACHKAY_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO5701459.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            862..1032
                     /locus_tag="ACHKAY_002801"
     CDS             862..1032
                     /locus_tag="ACHKAY_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO5701460.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            1106..1381
                     /locus_tag="ACHKAY_002802"
     CDS             1106..1381
                     /locus_tag="ACHKAY_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701461.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            1480..4131
                     /gene="recE"
                     /locus_tag="ACHKAY_002803"
     CDS             1480..4131
                     /gene="recE"
                     /locus_tag="ACHKAY_002803"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO5701462.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            4124..4933
                     /gene="recT"
                     /locus_tag="ACHKAY_002804"
     CDS             4124..4933
                     /gene="recT"
                     /locus_tag="ACHKAY_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO5701463.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4990..5184
                     /gene="ralR"
                     /locus_tag="ACHKAY_002805"
     CDS             4990..5184
                     /gene="ralR"
                     /locus_tag="ACHKAY_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO5701464.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            5177..5365
                     /locus_tag="ACHKAY_002806"
     CDS             5177..5365
                     /locus_tag="ACHKAY_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO5701465.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            5472..5753
                     /locus_tag="ACHKAY_002807"
     CDS             5472..5753
                     /locus_tag="ACHKAY_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5701466.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            5719..6795
                     /locus_tag="ACHKAY_002808"
     CDS             5719..6795
                     /locus_tag="ACHKAY_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701467.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(7188..7529)
                     /gene="yebY"
                     /locus_tag="ACHKAY_002809"
     CDS             complement(7188..7529)
                     /gene="yebY"
                     /locus_tag="ACHKAY_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO5701468.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(7542..8414)
                     /gene="copD"
                     /locus_tag="ACHKAY_002810"
     CDS             complement(7542..8414)
                     /gene="copD"
                     /locus_tag="ACHKAY_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO5701469.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(8418..8792)
                     /gene="yobA"
                     /locus_tag="ACHKAY_002811"
     CDS             complement(8418..8792)
                     /gene="yobA"
                     /locus_tag="ACHKAY_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO5701470.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8931..9161
                     /gene="holE"
                     /locus_tag="ACHKAY_002812"
     CDS             8931..9161
                     /gene="holE"
                     /locus_tag="ACHKAY_002812"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO5701471.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            9263..9919
                     /gene="yobB"
                     /locus_tag="ACHKAY_002813"
     CDS             9263..9919
                     /gene="yobB"
                     /locus_tag="ACHKAY_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO5701472.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9943..10605
                     /gene="exoX"
                     /locus_tag="ACHKAY_002814"
     CDS             9943..10605
                     /gene="exoX"
                     /locus_tag="ACHKAY_002814"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO5701473.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(10602..12662)
                     /gene="ptrB"
                     /locus_tag="ACHKAY_002815"
     CDS             complement(10602..12662)
                     /gene="ptrB"
                     /locus_tag="ACHKAY_002815"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO5701474.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12871..13530)
                     /gene="yebE"
                     /locus_tag="ACHKAY_002816"
     CDS             complement(12871..13530)
                     /gene="yebE"
                     /locus_tag="ACHKAY_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5701475.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(13857..14213)
                     /gene="yebF"
                     /locus_tag="ACHKAY_002817"
     CDS             complement(13857..14213)
                     /gene="yebF"
                     /locus_tag="ACHKAY_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO5701476.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(14280..14570)
                     /gene="yebG"
                     /locus_tag="ACHKAY_002818"
     CDS             complement(14280..14570)
                     /gene="yebG"
                     /locus_tag="ACHKAY_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO5701477.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            14704..15882
                     /gene="purT"
                     /locus_tag="ACHKAY_002819"
     CDS             14704..15882
                     /gene="purT"
                     /locus_tag="ACHKAY_002819"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO5701478.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(15938..16579)
                     /gene="kdgA"
                     /locus_tag="ACHKAY_002820"
                     /gene_synonym="eda"
     CDS             complement(15938..16579)
                     /gene="kdgA"
                     /locus_tag="ACHKAY_002820"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5701479.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(16616..18427)
                     /gene="edd"
                     /locus_tag="ACHKAY_002821"
     CDS             complement(16616..18427)
                     /gene="edd"
                     /locus_tag="ACHKAY_002821"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO5701480.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(18662..20137)
                     /gene="zwf"
                     /locus_tag="ACHKAY_002822"
     CDS             complement(18662..20137)
                     /gene="zwf"
                     /locus_tag="ACHKAY_002822"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO5701481.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            20475..21344
                     /gene="hexR"
                     /locus_tag="ACHKAY_002823"
                     /gene_synonym="yebK"
     CDS             20475..21344
                     /gene="hexR"
                     /locus_tag="ACHKAY_002823"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO5701482.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            21472..22914
                     /gene="pyk"
                     /locus_tag="ACHKAY_002824"
     CDS             21472..22914
                     /gene="pyk"
                     /locus_tag="ACHKAY_002824"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO5701483.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(22974..23816)
                     /locus_tag="ACHKAY_002825"
     CDS             complement(22974..23816)
                     /locus_tag="ACHKAY_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFO5701484.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(23839..24678)
                     /locus_tag="ACHKAY_002826"
     CDS             complement(23839..24678)
                     /locus_tag="ACHKAY_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5701485.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPLNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT         6707 a         6030 c         5905 g         6099 t
ORIGIN      
        1 caactttaat aactatgcag caggtttttg ttccgctacc ccggcgttaa ggggaaatga
       61 ggtcagcatg gatactatcg atcttggcaa caacgcatct ctggtgtacg gcgtgtttcc
      121 aaaccaggac ggcacgttca ccgcaatgac gtataccaaa agcaaaacgt ttaaaaccga
      181 aaatggtgcc cgtcgctggc ttgccaaaaa taccagctaa accatttatt ggattaattc
      241 aatattcttg ctgtaggggt atagccgagg ccaccaaagc ccggaggtgg tgaaataaaa
      301 ccgggcacaa cacgaaggcg catttccggt atccataaag agtcggtctt gtctgttaaa
      361 tttaaatggt gggagtgcgc ctccggttgt gaataacaac actgctgtgt gtagtcttgg
      421 cggcatcagt tttttcttga agttcgactg atgtccgccc tttttaaagt gaattttgtg
      481 atgcggtgaa tgcggctaag cgcacgcggc acagttaaaa gtcatgttag tccttattgg
      541 tttgggtggg aaagccgact gtaattgtta actggttgca gtcacctgga ggcaccaggc
      601 accgcatcaa caaagttcat ttgtaaaaat ggagataatt atgattgcac atcacttcgg
      661 aactgatgaa ataccacgtc agtgtgtgac tcctggtgat tatgttcttc atgaaggccg
      721 gacatatatc gcctcggcaa acaatattaa aaagcgaaaa ctttatattc gtaacctgac
      781 tacaaaaaca tgcatttctg actgcatgat taaagtcttc ctcggtcgtg atggtttacc
      841 tgtaaaggcg gagtcatggt gatgactaag aaaataaaat gtgcttacca cctttgcaat
      901 aaaggaattg aagaaagcaa aagcattaaa agaccacttc atttcatgcg tggagttatc
      961 ccaacgacgg aaatgaaaaa atattgtagt gaaaattgtg ccgaaaaaga ccagatggca
     1021 cacgaacttt aattaactga ctatgcgaaa ctgaatttat gccagcaatg gcagggattc
     1081 gctcaacctt aattaaggag aaaaaatgat taccaattat gaagtcactg ttgtaactac
     1141 cgatgacatt gttcacgagg ttaatctgga aggaaagcgt attggctacg tgattaaaac
     1201 agaaaataaa gaaaccccat tcactgtggt tgatattgac ggtccatcag gcaacgtaaa
     1261 aacacttgat gaaggtgtca caaaaatgag tctggttcac atcggaaaga atctgcccgc
     1321 agaaaaaaaa gccggatttc tggcaactct gattgcaatg aaattaaaag gtgaaatctg
     1381 aaaaaagaaa gcctgcacaa cgtgcaggcc tgagtgaaga acctgggaca tttattcatc
     1441 actcgcagta attttaatct gagttgaggt taaaaaacaa tgagcacaaa accactcttc
     1501 cttttacgga aagcgaaaaa atcatccggt gaacctgacg tcgtcctgtg ggcaagcgac
     1561 gattttgaat caacctgtgc cactctggac tacctgatcg ttaagtcagg taaaaaactg
     1621 agcaactatt ttaaagctgt tgccacaaat tttcctgtcg ttaatgacct tccccctgaa
     1681 ggtgagatcg attttacctg gagtgaacgc tatcaactca gcaaagactc catgacctgg
     1741 gaactaaaac cgggagcagc gccagacgac gttcaccatc aggaggatgc accggaaacc
     1801 gaagaaccga cgggaggcca ggaagaaaac gcgcaggcag acgcccacga ggattgccag
     1861 gattgcgaag tctctgtagc cactttgcgg ttcacacagc gtcttctgca catttttacg
     1921 tatgcagccg gggatcgaaa atacctgcat catgccaccc gtgaacaacg caaacacatt
     1981 actgctcttg agatggatca ggaaaacagc tatgtccaga atctgctgtt ggccatacgc
     2041 agcatggcag aaccgacaac tctggataat gccgccctgc tccgcctgac tgatgcaatt
     2101 aaggcagtgt tctctatcac gaaaaaacat cagccctatg aatttaagaa tttcatttca
     2161 gcctggctgg ataccgaaca cattgatcgc ggtcttctga caaaagaatg gcgaaaaggg
     2221 aatcgtgttt cacgcatcac tcgcacggct tccggtgcta atgctggcgg cgggaacctc
     2281 accgatcgcg gcgaaggttt cgtccacgat ctgacatcac tggcgcgcga tgtagccact
     2341 ggcgtactgg cccgttcaat ggacgtggac atctataacc ttcatccggc acacgctaaa
     2401 cgcattgagg aaattatcgc tgaaaataaa ccaccctttt ctgttttccg cgacaaattc
     2461 atcaccatgc ctggcgggct ggattattcc cgcgccatcg tggttgcgtc cgtgaaagaa
     2521 gcaccaattg ggatcgaggt catccccgca cacgtcactg aatatctgaa caaagtactg
     2581 actgaaaccg atcatgccaa ccctgatccg gaaatcgtgg atattgcctg cggtcgctcc
     2641 tcggccccga tgccgcaacg agtaacagaa gaaggaaaac aggacgatga agaaaaacca
     2701 caaccatctg gcgcaatggc agatgaacag gcaacggctg aaacagtgga accgaatgca
     2761 actgaacatc atcagaacac gcagccgctg gatgctcagt cacaggtaaa ttctgttgat
     2821 gcgaaatatc aggaactgcg ggcagaactc catgaagccc ggaaaaacat tccatcaaaa
     2881 aatcctgtcg atgccgataa attgcttgct gcatcacgtg gtgaatttgt tgacggaatt
     2941 agcgacccga acgatccgaa atgggttaag gggatccaga ctcgcgattc tgtgtaccag
     3001 aaccagccag aaacggaaaa aatcagcccg gatgcgaaac aacctgagcc agtagtgcaa
     3061 caggaaccgg aaatagtctg caatgcctgc ggtcagactg gcggggataa ctgccctgac
     3121 tgtggtgcgg tgatgggcga cgcaacatac caggaaacat tcggtgaaga gaatcaggtt
     3181 gaagctaagg aaaaagatcc ggaggaaatg gaaggcgctg aacatccgca caatgagaat
     3241 gctggcagcg atccgcatcg cgattgcagt gatgaaactg gcgaagtcgc agatcccgta
     3301 atcgtagaag acatagagcc aggtatttat tacggaattt cgaatgagaa ttaccacgcg
     3361 ggtcccggcg tcagtaagtc tcagctcgat gacattgctg atactccggc actgtatttg
     3421 tggcgtaaaa atgcccccgt ggacactaca aagacaaaaa cgctcgattt aggaaccgct
     3481 ttccactgcc gggtacttga accggaagaa ttcagtaacc gctttatcgt agcacctgaa
     3541 tttaaccgcc gtacaaacgc cggaaaagaa gaagagaaag cgtttctgat ggaatgcgca
     3601 agcacaggaa aaacggttat cactgcggaa gaaggccgga aaattgaact catgtatcaa
     3661 cgcgttatgg ctttgccgct ggggcaatgg cttgttgaaa gcgccggaca cgctgaatca
     3721 tcaatttact gggaagatcc ggaaacagga attttgtgtc ggtgccgtcc ggacaaaatt
     3781 attcctgaat ttcactggat catggacgtg aaaaccacag cggatattca acgattcaaa
     3841 acggcttatt acgactaccg ctatcacgtt caggatgcat tctacagtga cggttatgaa
     3901 gcacagtttg gcgtgctgcc aactttcgtt tttctggttg ccagcacaac tgttgaatgc
     3961 ggacgttacc cggttgagat tttcatgatg ggcgaagaag caaaactggc aggccagcag
     4021 gaatatcacc gcaatctgag gaccctggct gactgcctga ataccgatga atggccagct
     4081 attaaaacgt tatcactgcc ccgctgggct aaggagtatg caaatgacta agcaaccacc
     4141 tatcgcaaaa gccgatctgc aaaaaaccca gggaaaccgt gcaccagcag cagtaaaaaa
     4201 taacgacgtg atcagcttta ttaatcagcc atcaatgaaa gagcaactgg cagcagctct
     4261 cccacgccat atgacggctg aacgaatgat acgtatcgcc accacagaaa ttcgtaaggt
     4321 tccggcgcta ggaaactgtg acaccatgag ttttgtcagt gcgatcgttc agtgttcaca
     4381 gctcggcctt gagccaggta gcgccctcgg ccacgcatat ttactgcctt ttggtaataa
     4441 aaacgaaaag agcggtaaaa agaacgttca gctaatcatt ggttatcgcg gcatgattga
     4501 tctggctcgc cgttctggtc aaatcgccag cctgtcagcc cgtgttgtcc gtgaaggtga
     4561 cgagtttagt tttgaatttg gccttgatga aaagttaata caccgcccgg gagaaaacga
     4621 agatgcacca gtgacccacg tctatgctgt cgcaagactg aaagacggag ggactcagtt
     4681 tgaagttatg acgcgcaaac agattgaact ggtgcgcagc cagagtaagg ctggtaataa
     4741 cgggccatgg gtaactcact gggaagaaat ggcaaaaaaa acggctattc gtcgcctgtt
     4801 taaatacctg cctgtctcaa ttgaaatcca gcgtgcagta tcaatggatg aaaaggaacc
     4861 actgacaatc gatccggcag actcctctgt attaaccggg gaatacagtg taatcgataa
     4921 ttcagaagaa taattcagcc tggcggtgta atgcaccgcc aacgtgagac agtttttatg
     4981 acaaaaatta tgagatatga cgatgttaaa ccatgtccgt tttgtggttg tccatctgtt
     5041 acagtgaaag caatttcagg atattaccgg gcaaaatgca acggatgcga atcccgaact
     5101 ggctatggtg gaagtgaaaa agaagcactc gaaagatgga ataaacgaac tactggaaat
     5161 aataatggag gtgttcatgt ataaaataac tgccactatt gaaaaagaag gtggcactcc
     5221 tactaactgg acaagatatt caaaatctaa attaacgaaa tcagaatgcg aaaaaatgct
     5281 ctcagggaaa aaagaagcag gcgtgtccag agagcagaaa gtaaagctga taaattttaa
     5341 ttgcgagaaa cttctgtcct cgtgagttgc attatataca aattagaact tcatagctga
     5401 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtaa
     5461 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
     5521 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
     5581 ctgaactaca cgggaggagt tacagggtgg ttccggaagc tttctatatc aacccaaata
     5641 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
     5701 tactggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttacc taaaaactta
     5761 acatatcgaa ggagggacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
     5821 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
     5881 atatacaaaa actactctcc tgctgcctta attgaaaaac taaaagggtt cgactcattt
     5941 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agtgtccaga
     6001 aatacttata aaattcgggt aaatcaactg gagacaataa aagaaaaatt aggaggggtt
     6061 ttactgacag aaataaccac tcgccatatt gccgagtttc ttgatttgtg gattgaagga
     6121 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaggcc
     6181 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
     6241 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
     6301 cttccgacat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
     6361 gacataacaa atatgcggtt cagtgagatt tatgatgatc gtctccacat caggcaaatt
     6421 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     6481 cttggtacag tagttgaaca gtgccgcatg gtaagtaggg gggattatct aatcagtgcc
     6541 gggattagaa aaaacagccc tgacggcagc attcacccgg acggcctgac aaagaaattt
     6601 gtcgcagcca gaaaattaac aggtatccag ttcagtgaaa acccaccaac ttttcacgag
     6661 atcagaagcc tggctggacg attgtacaaa gaaacatgtg gggaagaatt tgctcaacgt
     6721 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     6781 tacttactgc tctgatttta acgtaaatgg attgttaaat gtgttttggt tgtgatataa
     6841 ccaaaaaaga ccggaataca gaaattcgag taaatttcga gtaatttcgg ggagacgttt
     6901 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     6961 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     7021 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     7081 tcaataaaaa gcgtggtggt catcagctga aatgttaaaa accgcccgtt ctggtgaaag
     7141 aactgaggcg gtttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     7201 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     7261 atctgaatca ccgaaatggg ttgggcttta gtcttcccac tggcaacttc cttttgtgcg
     7321 atatcgttta aaggatactg cacgagggta cttgggttaa tgacatacaa agcattaccc
     7381 ggtctgcaag tcagcatcac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     7441 aaacgactga cggtaatcac ctgcggtgca gccagcgccg ctgcagaact ggtgagtaac
     7501 agaaacgtca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     7561 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     7621 cactcaaacc agggtgccgc acgatcttca tcttgcctca tgcgcggtac aagaacataa
     7681 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     7741 tggccccagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     7801 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     7861 tgaatggcct ggtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     7921 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     7981 ttcgccgtta ccccttcatt cagtgtcgca tgcccgattc ccgccagcag aataaattgc
     8041 gcggtagtga gcataaacag caaacgtggc atattccgcg gttgcataag ggcgacgatc
     8101 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     8161 acagcctgcc aaatgtttgg cgaaaataca tctgcccatc ccgttcccat caacccgccc
     8221 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     8281 cgcagaaaac gcttcgccag caagcgacga atcgttaagg gggccagcca ggcaccgtac
     8341 attgcgaagc caaagaccag catcagcgag gtgaaatgga taaatcgcag cgcgatccag
     8401 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     8461 tccaccgaaa caacatgcca gtcaacgatg taggttccgg gtttcagcga atcggccagt
     8521 ggaacaatca gttgcttttg gtcctgctca tttcgcttcg ccggtaatgt tttaatattt
     8581 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacacc ttccgagaag
     8641 tttaaagtga ttgcctgcgg tgcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     8701 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgccagc
     8761 gcgtagcgaa gggagcgtgc agttgaagtc atattatcta ttcctttttg taataacttt
     8821 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     8881 cctggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     8941 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     9001 gggtggcatt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     9061 agcctgaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     9121 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     9181 tttcttacat aacgcctgct aaattatgag tattttctaa accgcaccca taatttgcag
     9241 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     9301 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     9361 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     9421 gcgacgtgcc cttcctgccc cacccgatct ctcactgtta gacccgcttt gttatgccgc
     9481 aacgacctgg cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     9541 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggatctacc atcagagtca
     9601 tggtgcatgt ctgggtcgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     9661 tatggatatg gacccgacct gttcactgtt tactacaggg caatgtctgg gagaacccga
     9721 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     9781 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     9841 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     9901 tatcattcca ttacgctagg ctttttcggc ctggagcatg ccatgttgcg cattatcgat
     9961 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
    10021 ggaaaaatcg tcaaccccat gagccacctg gtgcgccccg atcgtcctat tagtccacaa
    10081 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
    10141 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc ataacgccag ctttgaccgc
    10201 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
    10261 tggcctggga tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
    10321 acgccgccgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
    10381 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
    10441 cgtccgtcgt tgatgacgac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
    10501 gttgccgaac gcgatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
    10561 ccggagctgc gtttaacact gaaacattat ctggaaaata cttagtccgc aggcgtagcg
    10621 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
    10681 gatttaaagc gaccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
    10741 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat
    10801 tgcacctgag aatcgtgcaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
    10861 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggatta
    10921 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
    10981 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
    11041 gcaacgccca tcaacatgcc ccccgcactc ccgcccatcg cataacaaag cgaaggagag
    11101 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
    11161 ttcttcagaa attttccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
    11221 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
    11281 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
    11341 cgaaaatgtt tgcgatggta gaccaacgaa accggaactt cgacgccatc acgggcgact
    11401 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac cgggcacttc tgtttgtttt
    11461 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
    11521 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
    11581 acataggctg gatcatcaaa ggcaatacca atgacttccc gggtcttgcg gttaatttgg
    11641 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
    11701 aacccttcca gcatgatgtt ttcgcgtggc ggaattaact cttcccactg ttgctcatca
    11761 cgcatacggg tacggtataa gccaaagttt ttgccgtggc ggttggaacg tagataaaaa
    11821 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
    11881 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
    11941 aaatgaatga ctacatagtg cttcgaggtc gttttatgca ggctgacgta ataggtatcg
    12001 tctttttctt cgtagatcag tttatcttgc gatgctggcg taccgatggc gtgacgccag
    12061 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
    12121 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttaccagtt
    12181 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
    12241 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
    12301 gcgcgcttat tggcatcgag caatgtttcc cactcatccc actcttcact gaatgccgat
    12361 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
    12421 tttttgatgt agggcgcaga aacttctcgt tgcggaatgc ggtcgatgat ttcctttaag
    12481 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
    12541 tgcaggtagt ccaggacttc tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
    12601 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
    12661 atgttattgt tctttctggt tgaaacatct tataagggtg gcaaaactca ccggggatgc
    12721 aagcgaaaca ggggagtcat tgcttagatg atgacaggta atggcgcgga tatcgaatgt
    12781 tatgcagaca gagaaaatca gcctgttcaa atggctgtgc gattctggat agcccgaaat
    12841 agtcaacttc aggctatcca gagagcggaa ttattccgcc aaagtgcgtt tttgctgttc
    12901 gagatcgcgt tcaatgccgt cacgaacatc ctgggggatt ttcagcgcgt cacccagtgc
    12961 attcaggtaa ctgcgttcca taaaatggtc aatatcaata gccgcgcaac tcaggaaata
    13021 gatttccagc gcctcttctt cattgcggac cccggtagcc aggcgttgtg gatccagcgg
    13081 ttgttcgatt gcctgctcaa tgagtacacg cccctgctct tccacgccgg cttcacgcaa
    13141 ttgctggtcg attgccgcac gttctttggc atcaatatga ccatcacttt tagcggcaaa
    13201 gaccagcgca aggatcaaac gttctgtacg ctcatccagc ggcgtacttt gcgcgccaaa
    13261 ctgcggttcg tcctgatgcg ccgcgcgaat tttatctttg tatttattcc acagcaccgt
    13321 acccgctacc gctccgccgc caaccagtaa cgcgttggtg ccatatttcg taagaagttt
    13381 acgtgctgat ttatttgcga ccagcagccc agccagtccg cctaatgcgc ctggcactaa
    13441 cagtttgccc aatccctgat ccgcagacga ggaggtagaa gaactgcttt gcccaagcag
    13501 ggattgcagt tgatttaacc agttagccat atttgctcct caataaccat ttttagtgcc
    13561 tgtagcatag cggatgagga tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa
    13621 gcggggcggt aggccgttat tcaaagaaat tcgcgccagg taaggtattt actgtatatc
    13681 aggtgataag ggtattttag gtgaaagtag tttgtcgttt tgagtggcat tgtttgatgc
    13741 tgattgcccg atgcgacgct gttgcgtctt ctcaggccta cgattgtggt gcgttctggg
    13801 gcggacaagg cactcgcgcc gcatccgcca acggtcaatc gaattatcag aagccattaa
    13861 cgccgctgat attccgccat tcccgctttg cagtccacgc tgacctggta atgaatatcg
    13921 gcacttttac cacgcacggt tagcggtact gaccatttat catctttacc ctgaatgtcc
    13981 tgcaaactga cccaagccac gggatcggcc tgaccgacaa ttttttgatc atctgcccaa
    14041 cgcgccacgc gattttgttg ataatcacgt tttacgctcg cggcaattcc ggcagcatcc
    14101 agaccttcac actttgggaa agtgaccgac ttgctggttt cattattggc agcgaaaact
    14161 gatgcgcagg cagaaaccaa caacagccct aaaaacgccc ctcttttttt catgtttttc
    14221 tccatagcac aatgattcag gagaaagcat ggtacaaatt gtcaggagcg caagttgctt
    14281 caggccgcgt gtgaggcatc ttcctcttcg gattcagcac cgacgacctg tggggacggt
    14341 aatttgccgg ttttcagaat ggtgctcagc acatcttttt gttctgccag ccatagcgaa
    14401 agggcttcac gttgctcgtc ttccatttgc actggagaat ttgtcagcca ggtgtcaagc
    14461 agatccgccg tatcaagcat tttatcataa gcatcggctt cctttttgct ggtaaacgac
    14521 attttctctt cgccctcacg aatgactacg tatttaactt caaccgccat ttgcagcctc
    14581 tcataataac tgtgatttta tacagtatat ttcttttcgg ttgagaaatc aacatcagca
    14641 ataaagacac acgcaaacgt tttcgtttat actgcgcgcg gaattaatca ggggatattc
    14701 gttatgacgt tattaggcac tgcgctgcgt ccggcagcaa ctcgcgtgat gttattaggc
    14761 tccggtgaac tgggtaaaga agtggcaatc gagtgtcagc gtctcggcgt agaggtgatt
    14821 gccgtcgatc gctatgccga cgcaccagcc atgcatgtcg cgcatcgctc ccatgtcatt
    14881 aatatgcttg atggtgatgc attacgccgt gtggttgaac tggaaaaacc acattatatc
    14941 gtgccggaga tcgaagctat tgccaccgat atgctgatcc aacttgaaga ggaaggactg
    15001 aatgttgtcc cctgcgctcg cgcaacgaaa ttaacgatga atcgcgaggg tatccgtcgc
    15061 ctggcggcag aacagctgca gctgcccact tccacttatc gttttgccga tagcgaaagc
    15121 cttttccgcg aggcggttgc cgccattggc tatccctgca ttgtaaaacc ggtgatgagc
    15181 tcttccggca aggggcagac gtttattcgc tctgcagagc aacttgctca ggcatgggaa
    15241 tacgctcagc aaggcggtcg cgccggagcg ggccgcgtaa ttgttgaagg cgtcgttaag
    15301 tttgacttcg aaattaccct gctaaccgtc agcgcggtgg atggcgtcca tttctgtgca
    15361 ccagtaggtc atcgccagga agatggcgac taccgtgaat cctggcaacc acagcaaatg
    15421 agcccgcttg cccttgaacg tgcgcaggag attgcccgta aagtggtgct ggcactgggc
    15481 ggttatggtc tgtttggtgt cgagctgttt gtctgtggtg atgaggtgat tttcagtgag
    15541 gtctcccctc gtccacatga taccgggatg gtgacgttaa tttctcaaga tctctcagag
    15601 tttgccctgc atgtacgtgc cttcctcgga cttccggttg gcgggatccg ccagtatggt
    15661 cctgcagctt ctgccgttat cctgccacaa ctgaccagtc agaatgtcac gtttgataat
    15721 gtgcagaatg ccgtaggcgc aggtttgcag attcgtttat ttggtaagcc ggcaattaat
    15781 ggcagccgtc gtctgggggt ggcactggct actgcagaga gtgttgttga cgccattaaa
    15841 cgcgcgaagc acgccgccgg acaggtaaaa gtacagggtt aaacccggca aaaaaacgct
    15901 acaaaaatgc ccgatccgcg atcgggcatt ttgactttta cagcttagcg ccttctacag
    15961 cttcacgcgc cagcttagta atacggtcgt aatcgcccgc ttccagcgca tctgccggaa
    16021 ccagccagga accaccgatg cacagcacgc ttttcagcgc caggtagtca cggtagttag
    16081 ccggagaaat accacctgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    16141 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    16201 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    16261 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    16321 ccgcttcggt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    16381 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    16441 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc actgcgtgtt
    16501 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    16561 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    16621 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    16681 attcacgtcc tgtccccacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    16741 ccagttccgc ttcgtctacc agcagcgtca gttcgcctgt ctgtccattc acacgaatga
    16801 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    16861 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    16921 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    16981 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    17041 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    17101 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    17161 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    17221 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    17281 gggtataacg agacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    17341 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    17401 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    17461 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    17521 ggttagtgga accaccggtc gccagcagtg caacgatacc gttcaccacc actttctcat
    17581 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    17641 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    17701 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    17761 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    17821 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    17881 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    17941 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    18001 tatgggacag cccgaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    18061 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    18121 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    18181 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    18241 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    18301 cgcatgccaa ctgcgaacga tgaacggtcg aagttttcgc ttgttctatc cgggcgagat
    18361 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    18421 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    18481 tcagaggatt tatgactgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    18541 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    18601 ttctacaatc tgtgcaagat catgttaccg gtaaaataac cataaaggat aagcgcagat
    18661 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    18721 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    18781 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgaaca aacagtgcct
    18841 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    18901 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    18961 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    19021 tcagtttatt ctgcggcaga tcctgccacg attctttaaa cagattcagt tcaggtgttt
    19081 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    19141 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgctacg aaggtttctg
    19201 tattgctgct cttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    19261 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    19321 ggcgcagaga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    19381 acggcggaga catcgcaatc atgcagagaa tttgcagcag gtggttttgg atcatatcgc
    19441 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    19501 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    19561 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    19621 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    19681 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agtttagcct
    19741 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatgg
    19801 tgatacgatt tttttgatcc agcatcgcgc cgagacggct gaatgcagca gtgtcattga
    19861 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    19921 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    19981 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca
    20041 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    20101 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    20161 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttac
    20221 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacggtaaaa gcgcgcaaag
    20281 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattggg taaaaatctg acactgatca
    20341 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacaca gcacacaggc
    20401 gtaatatctg acaaaactac atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    20461 agtttcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    20521 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    20581 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    20641 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    20701 atggcactcc ctatgttaat cgcaatgtca atgaagatga cagcgttgaa tcatacacag
    20761 ggaaaatatt tgagtccgca atggcaacgc ttgatcatgt ccgtcattca ctggataaat
    20821 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    20881 taggctcttc agccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    20941 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    21001 gagacgtggt ggtgctgatt tctcacactg gaagaacaaa aaatctggtc gagctggcgc
    21061 agctggctcg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    21121 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    21181 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    21241 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    21301 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaaacga ctgtcactgt
    21361 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    21421 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaga
    21481 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    21541 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    21601 cctgaagatc acaaaatgcg cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    21661 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    21721 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
    21781 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgttgtgcct
    21841 ggcgacatcc tgctgctgga cgatggtcgc gttcagttaa aagtactgga agttcagggc
    21901 atgaaagtgt tcaccgaagt gaccgtcggt ggtcccctct ccaacaataa aggtatcaac
    21961 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    22021 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg cggcgaagat
    22081 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    22141 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    22201 gacgtggtaa tggttgcacg cggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    22261 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctta accgcgcggt aatcacggcg
    22321 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    22381 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    22441 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    22501 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    22561 gctattgcca tgtcagcaat gtacgcagct aaccacctga aaggcgttac ggcgattatt
    22621 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    22681 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    22741 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    22801 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac ccagggcgac
    22861 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaatctcac
    22921 ctgccccggc cgctgatgac cggggtattt agcaacacat aaattaggac tactcattca
    22981 atccaatcat aatcacagcc ccctgtaaaa tgaatacagg gatagcctga atggagatct
    23041 aaatagcttg caagacgtcg catacccgct tcctctgcaa cactatatcc aataactgcc
    23101 atcggtaagc cactatcttt ggagtattgt gtctgcaccc atgttttaat tgaatcgtta
    23161 atcgtaatat aataatcagc ctgatattcc atatattgta ttggatcaca aaaacagcct
    23221 gccccaatcc caatagttct aactattctg gctctatctc cataaaattg gatcccagga
    23281 agattataaa ttttcaaatt tttggcaaaa cgtttggtaa tattaatggc actgtcaggt
    23341 tctatagcat atacatggta catatcatca gaagcaacaa tatttgtaac atccagttct
    23401 aactgctgag ccaaagcctt agacattccg aacgtaggct ctcgatccat gacatcatgg
    23461 caacgaatga ttaccaaatt atttttatgc aaaaactcct ttttttgctc aattattttt
    23521 aaataactga gttcaccgcg cgtataatcc attatttttc tggcccatga aaactcaaga
    23581 acgtcaccct ccagatcagc ataagaataa aatgtaggct catgagtaat aatgatattg
    23641 gcaccttcaa aaacagcctt tcttaaaaca tcaattgagg caatccagca ggtcccaatt
    23701 ttattaataa gtgtcgcctc agcaccaaaa ataattttat caacagttgg ttgcgaaacg
    23761 gcaaccattg aatttaaaaa acaattaaga tctgcggctg ttttaatatg atgcattcaa
    23821 catccttcac cggtttcatc agcaataaaa taatttcaac atttctggaa aacgttttgc
    23881 ccatgcagac tcagagtggg tatcacctcc ccatagatga taatctatag ttacattgcg
    23941 ttgtttacgg agactcacgc ataatttttc cgcgcctgaa atatagactt caggaaagtc
    24001 ttcgcgcata ccactggttt ccagcgtacc gacatcgaga tatacagtaa gatctgcaaa
    24061 gatattgttt tcctgaataa aattcagcat tgcgctgtca ttgaaccata gtgccggcga
    24121 aaaaacgcca acttttgaaa aaacgtctag acgaagaatt gcggcaaaca aactgatgta
    24181 cgcacccata gaactacctg ctatcgctgt attattacga tctgggagag ttcgaaaatt
    24241 gctgtcaata tagttcttga gcgtattcac aataaaatca acatacaacg caccttttcc
    24301 tccactataa tccacatcag ggtcccagcc aggtaaggga aaagatgtgt ttagcggcca
    24361 tggagaatac tcatccatac ggctgaattt accatgagac gatgcattat caatccccac
    24421 aatgataaca ccgtcgtatt gggaatttgg gaaaaaagca tctaccgcct caggtatatc
    24481 ccatacatgt ggaccaaaag atacagattt atcaaataaa ttttgcccat cgtgcatata
    24541 caatacaggg taactgacat cagattcata ataggacgca ggtagtaata ttcttatttc
    24601 gcgactactt gccaattgtg gcatcggaaa ggattgcaac acatgatatc gattcccata
    24661 gttctcaata tcgttcataa tttaaattaa cctcaataaa caaaagaaat attacatcta
    24721 agcactcccc taccacatta t
//
LOCUS       DAZFKM010000068        24730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFKM010000068 DAZFKM010000000
VERSION     DAZFKM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHKAY_002827"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACHKAY_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO5701486.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACHKAY_002828"
     CDS             complement(1899..2636)
                     /locus_tag="ACHKAY_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701487.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHKAY_002829"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACHKAY_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO5701488.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3619..4104)
                     /locus_tag="ACHKAY_002830"
     CDS             complement(3619..4104)
                     /locus_tag="ACHKAY_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO5701489.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            complement(4120..6942)
                     /gene="traG"
                     /locus_tag="ACHKAY_002831"
     CDS             complement(4120..6942)
                     /gene="traG"
                     /locus_tag="ACHKAY_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO5701490.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(6939..8312)
                     /gene="traH"
                     /locus_tag="ACHKAY_002832"
     CDS             complement(6939..8312)
                     /gene="traH"
                     /locus_tag="ACHKAY_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO5701491.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="ACHKAY_002833"
     CDS             complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="ACHKAY_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO5701492.1"
                     /translation="MKEKRNDAELKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(8672..9019)
                     /gene="trbJ"
                     /locus_tag="ACHKAY_002834"
     CDS             complement(8672..9019)
                     /gene="trbJ"
                     /locus_tag="ACHKAY_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO5701493.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="ACHKAY_002835"
     CDS             complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="ACHKAY_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO5701494.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="ACHKAY_002836"
     CDS             complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="ACHKAY_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO5701495.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(9884..10228)
                     /gene="trbA"
                     /locus_tag="ACHKAY_002837"
     CDS             complement(9884..10228)
                     /gene="trbA"
                     /locus_tag="ACHKAY_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO5701496.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(10242..10985)
                     /gene="traF"
                     /locus_tag="ACHKAY_002838"
     CDS             complement(10242..10985)
                     /gene="traF"
                     /locus_tag="ACHKAY_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO5701497.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(10978..11235)
                     /locus_tag="ACHKAY_002839"
     CDS             complement(10978..11235)
                     /locus_tag="ACHKAY_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO5701498.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11262..13112)
                     /gene="traN"
                     /locus_tag="ACHKAY_002840"
     CDS             complement(11262..13112)
                     /gene="traN"
                     /locus_tag="ACHKAY_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO5701499.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13109..13747)
                     /gene="trbC"
                     /locus_tag="ACHKAY_002841"
     CDS             complement(13109..13747)
                     /gene="trbC"
                     /locus_tag="ACHKAY_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO5701500.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13756..14061)
                     /locus_tag="ACHKAY_002842"
     CDS             complement(13756..14061)
                     /locus_tag="ACHKAY_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701501.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKNADISEQVTLVRLYLKVTFTCAFVAVFLNYLFSDEFMPVNSRK"
     gene            complement(14091..15083)
                     /gene="traU"
                     /locus_tag="ACHKAY_002843"
     CDS             complement(14091..15083)
                     /gene="traU"
                     /locus_tag="ACHKAY_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO5701502.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15080..15712)
                     /gene="traW"
                     /locus_tag="ACHKAY_002844"
     CDS             complement(15080..15712)
                     /gene="traW"
                     /locus_tag="ACHKAY_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO5701503.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(15709..16095)
                     /gene="trbI"
                     /locus_tag="ACHKAY_002845"
     CDS             complement(15709..16095)
                     /gene="trbI"
                     /locus_tag="ACHKAY_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO5701504.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16092..18722)
                     /gene="traC"
                     /locus_tag="ACHKAY_002846"
     CDS             complement(16092..18722)
                     /gene="traC"
                     /locus_tag="ACHKAY_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO5701505.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(18848..19195)
                     /locus_tag="ACHKAY_002847"
     CDS             complement(18848..19195)
                     /locus_tag="ACHKAY_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701506.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(19223..19441)
                     /locus_tag="ACHKAY_002848"
     CDS             complement(19223..19441)
                     /locus_tag="ACHKAY_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701507.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            complement(19521..19997)
                     /locus_tag="ACHKAY_002849"
     CDS             complement(19521..19997)
                     /locus_tag="ACHKAY_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701508.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(19990..20211)
                     /gene="traR"
                     /locus_tag="ACHKAY_002850"
     CDS             complement(19990..20211)
                     /gene="traR"
                     /locus_tag="ACHKAY_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO5701509.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(20346..20861)
                     /gene="traV"
                     /locus_tag="ACHKAY_002851"
     CDS             complement(20346..20861)
                     /gene="traV"
                     /locus_tag="ACHKAY_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO5701510.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(20858..21178)
                     /locus_tag="ACHKAY_002852"
     CDS             complement(20858..21178)
                     /locus_tag="ACHKAY_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO5701511.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(21165..21731)
                     /gene="traP"
                     /locus_tag="ACHKAY_002853"
     CDS             complement(21165..21731)
                     /gene="traP"
                     /locus_tag="ACHKAY_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO5701512.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPVLPGKMKCIRVVTADEYLKDVYTEDAS
                     LPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(21721..23172)
                     /gene="traB"
                     /locus_tag="ACHKAY_002854"
     CDS             complement(21721..23172)
                     /gene="traB"
                     /locus_tag="ACHKAY_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO5701513.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPAPPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(23172..23900)
                     /gene="traK"
                     /locus_tag="ACHKAY_002855"
     CDS             complement(23172..23900)
                     /gene="traK"
                     /locus_tag="ACHKAY_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO5701514.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(23887..24453)
                     /gene="traE"
                     /locus_tag="ACHKAY_002856"
     CDS             complement(23887..24453)
                     /gene="traE"
                     /locus_tag="ACHKAY_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO5701515.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(24475..>24730)
                     /gene="traL"
                     /locus_tag="ACHKAY_002857"
     CDS             complement(24475..>24730)
                     /gene="traL"
                     /locus_tag="ACHKAY_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO5701516.1"
                     /translation="QSRWFGLPLDELIPAAICVGWGITTSKYLFGIGAAVLVYFGIKK
                     LKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
BASE COUNT         5956 a         6834 c         5744 g         6196 t
ORIGIN      
        1 agcctgagtc acatcttctc cggaggcaac ctccggttca aagaggctgg ccatctgacg
       61 accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc tgatatttca gagagagttt
      181 gactgccgga tacggtccgg gcagagtgac atagcaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata ccccgtcacg
      301 taccgggtca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctcggggaa cggaaaaaag cacgggtgtt
      421 catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 aggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccacgca tccagtcacg gatggtgaag ggatcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     1021 cgggtcctct tttgtaccca tagggatcag cgtgttcgcg gtattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat
     1141 tttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcatagtt ggccagacga cggataacct ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtgatcagg caaataacca gggccacgac
     1501 agacgccaga acgaatgcag accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctgg gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc
     2581 ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagtt ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaaa ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac ctgatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 tacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccta caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctttatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcaattttt tcattttcat caatgttcct tcatattgaa taaaacgtca
     3601 taatatacaa attaagattc actcatcata tcgttttgaa ctataaccat atgaattacc
     3661 accgatatcc acaccaatac ctgacatagg taaccctgtt gcaggattag tacgatgaaa
     3721 atccatatta tcaaaatcca gatgcaaatc ttcattagtt tgttcattta cctgagattg
     3781 catccattct cttacgctta cacaaccact aactcgcctt aattcgtata gtgtaccaat
     3841 gataaaaacc atcaggctta ataatggaga gagtaaggaa gatactggca tcgttatata
     3901 ccaaacgcct ttactaaact taaactcatc cgacacattc cgtttttttc gtatttgaga
     3961 gaacaaccat ataaacagtc cctgccatag tactatgaag aacagcataa aaattgtttc
     4021 ggcattcccc caactagcca gataacaggc gataaactgt atcaccgtta ccagagtaat
     4081 atgggttaaa catctcatta tcatctccca aaaacagcat tattgcttat acttatcctg
     4141 atattccttt gcccttttca tcagttcctg tggactgtca gccccgggca ttctttcctg
     4201 agccgacttt tcttcattat atttattatt ctgagataat gcctctgttt tatggtgatt
     4261 ctgcagttca gaatactgtc ctcttacaat attttcctcg ccacgtacgc tgttctgagt
     4321 atcacctata ttccctttat attctgtgac catattatca acctgatgtt ttacatcatt
     4381 acggatatta ctgtcctgcg ttctttgatc aataatggcc tgatgcccct gatggtcagc
     4441 aataatatcc tgactgccac cacccgaagg tacgctctcc attcctttac caatatcacc
     4501 acgggattcc cgccaggcat tatcaacacc aggctgtacc tgttcctgca caaaagacca
     4561 ggccatcgcc cgacgacgtt ctgcaatctc cggtgaactg gtgtttgtca gaatagcttc
     4621 tgcatcctgt ggcgcgtgtt tcattacata ctgtgcaaac tgttgcgaca ggtcttcact
     4681 catctgccca ctcatgcttt cagtacggga tgccatttca gcatattcat ggctgcgggt
     4741 catattcgtc gtgtactggt cgtaactctg cttcgctgaa ttcagggctg cagacaactg
     4801 atccacacgg gaatcagcat tattgtccgt atggctgccg gactcactga ccttgcgact
     4861 ggtaaaataa tcgctggcct ctttaaagtc ttgtgtagct ctggcatcga tatcatgacg
     4921 agcattatgg ctcgactgag tattactact tgccgtatgc gcatcattat cttcccaatc
     4981 aataccagcc cgaccaccac ctctaacgcc taatcctcca ccaacaccaa gaattttagg
     5041 tttaaccccc cattcagcat gagcatctcc atacatcccg gcagaagctc tcgtacttct
     5101 ggatgccagt tcctgagtcg cctgctcatt actgatatta tgcgccttcg catagctttc
     5161 cactgcactg cgcatacggc tggccatcat ggagtcctgt gcgctcatcg tgctgtcagc
     5221 accacctgtg acagaatcac tgctcccccg gttagaacca aactggctga gcgagttcca
     5281 ggcactggca atactgctgc tgaacccatg cagggcgctt tctgctctgt tcgacgcctc
     5341 ccgggccatt tcctgttgtg ccgctgcaat ctgacgcgtt gcattgatac cgaccggtaa
     5401 ccgggacatc gccccgctcg catccatcac catattaccg tcacgggtct gtgtggcagt
     5461 tgcgccactg ccggtctggt acatcatctg accaaacgac gtggtgctgt tggtgctcca
     5521 gctgaagccg ttcacgtttt ccgtctgcat gttgccgtag gagtaattac cgtcaaccac
     5581 actgccagct gcactggcag tcgggctgat agcagaggaa gcgaaatggc tgtacacgct
     5641 ggaaaaacct gcgccaaggc cttttaccat catccatgac aacgggggta tcatgacaga
     5701 tatatatccg gcagtggacg ccaggtcgga gtatttcagc tgtatctgag agagttcaga
     5761 gagcacgacc ggtgcaccat tctgctttgc atagaatgtc atggcactgt tcagaatggc
     5821 atacagcaac ggccaggtct ggaaccacat cagagcaaac acatagcctc tcagaacaga
     5881 cagcgtcagt ctgttgaaga cagccgccag taccaggagc gggaagatac caatggtaat
     5941 gcccaccaga atggtctgca ccatcggcag gtttcgcatc agcacatgac caatggagac
     6001 atgtgccaga cgctgtttct ccatcgacga agtcgtggcc agactgacca ggctggcggt
     6061 atcaccgtta cgggctgcat tactggtgat accctctttc agggcattca tggtgacgtt
     6121 ctggcgcata atatgactgg cagactggct ggagccgtag aaataactgt aactgtcact
     6181 caccagttgc ctgaataaca ggtccgggtc cggtctgccg ccaaatatct gctgcacata
     6241 ataatgccag gtcttgcctc ccgtctttgt atcgagattc agcctgtctt tcagcgtgac
     6301 cgacgcatct ttacaggtta tgaaattatt attgctgtca taaacgcccc gtagcgggct
     6361 gggccgggaa aaaatcagcg tgtaggggtc agcggaggcc atcaggtctt ccagcgtgta
     6421 tttgtggttc aggtaaatat cccccagcac gcagttctgg acgtagtcct gaaacagatt
     6481 gatgatttcc ggattccggg acagaaaatc ggtgcttttc acaatcaggt tcgccccgaa
     6541 cagcatgcct gtcttgctgt aggtggcgct gtcaggctgg gtgaatatca tctcgtaact
     6601 ggccaccatt gcatgcccga tacgggtcgt cagtgaaagt ggcatcgcca gtccgacagg
     6661 cacattatcc acccggtgaa cctggaccag gtcactgtta tcaataatct gcaccgatgt
     6721 acggacatta accagcaggc tgataagcac aaacacggcc acccagccca gcaaatccat
     6781 cacgttgtgc cgctgtaccc acatcacggc caccgcgaga acagacaatg tgagcgcaat
     6841 tttttcgatg gaatcccacg tccgggtccc cataaaggcg gcaatggcgt tcaggttatt
     6901 gcgtaaccac tcaccaccgg caatcacata gacttcattc acagcgtgct ccctccgaag
     6961 tgatagttgt tctggtaacg gctgagcatg cgggcggaaa gctgctggcg catatagctc
     7021 atctgacggt caacaaccag caatgcatcc tgctggacct ttacctgccc ctggaaaacc
     7081 gcaatctgtt gctgtgcctg attcagattc tccagcacac tgtccagcac ggtctgtgga
     7141 tagttcccgg tggcaatcat ggcccgggcc tgctgaagca gttcctgaat gtactggagc
     7201 atgatgtcgt agccgatata gtccgtcagc tggtaaacca cgctgctgga tacaccgagc
     7261 atctgcgggt caataagata tttaaagacg gggacggtgg tgctggaaat gaagcctttt
     7321 tccctgtcat ccagtggagt atcactcacc gctttattct gaatactggt gagcagtttt
     7381 gtaatctgtg atttcagggc tttatcccgg gcgatggtca catttgcatc cgccaccacc
     7441 ttcagacact tgtccgaatc gttgcagtga taaacttttg ccgttccccc ctccataatc
     7501 gcccggagga tatcgcggtc agtggtacgc gccggcaggg gggtaatctc gctgtcttcc
     7561 ccaaaaatca gcgtaccggt gagcgtcata ataaattctt tcagttcttt gttaccatca
     7621 agcatcctgt ttttggacag cgagtcccag atgatattga tgttcttcag cacccgttcc
     7681 ttatctttgt cactggcctt gtcacgaaca gacgatgact gtcctccgac cgtgcagccc
     7741 tgacgggacg cggcccagtc agcaaaaata ttgctctcac cggcaatgtc ctgacagacc
     7801 ttctgctgtg acacctgagt ctgaggaaat aatcctccga caatcccctg cgccacctga
     7861 caggagctga ggttcatact gttaatgtcg cttgccattt tctgcaggaa gtcttttgcg
     7921 gttttgattt ccggcaccgt tgcctgcagg gcaaggtcaa aaaagtaacc ggcagcgttg
     7981 ctcataatct gcttaacaaa acgctgcaac tgatcgccat taataaaact gaacgagccg
     8041 agataggcgt cgatgccgcc acatccggca ttgatatccg gcagcgtcat ggaaatcagc
     8101 tggatatttt tgacctgggt gcgggcatac agggagccac cataggcata cccggcggcc
     8161 tgcccctgcc agacgcccgg ttgtgtggta ttggaagcaa atcccagctt attaaagaac
     8221 tgattcatgt cgctgttcac atccgctgac gctgcgggtg tgaccgtcag taaagcggca
     8281 caaagaacca gaagaggttt aatgcgaagc attattgcca ccctccctta atttcttctc
     8341 ccggcgcata cggcgtttca gccggaatat tctgatcccc ccgtttatta attcatttaa
     8401 agggttcctg acgtatacga taagaaatcc acctgcaaac gcccataacg tatacggaat
     8461 gacatacatc aggatgttcc acatataacg aaaattattt aattccgggt cagctttcca
     8521 cgaatcaata caggcatcaa aaatactatg cataatcacc cataaaaaag ttccggcagc
     8581 aacgaacaca aaaaacagat caaaatatat atcagaaacc ctgcgttcat aatcataatc
     8641 ccgtttagtt tttctgtttt ttagttccgc atcatttctt ttttctttca ttataagttt
     8701 cctctgatat ttccgatgat tcattcagac cgttcatgcg gctgtaatat atttcagaca
     8761 ccgaaatcca tcccacaatt gcccccacaa aaaacacaac agaaacagaa aaggtaagac
     8821 acagcgaaac gacaatttcc acagggctgg cgggataaaa aagcccctga cggataaagg
     8881 ggatattcag gctgacgatt attcccgtcg cgatgccgga tattccggca atgagtaaca
     8941 ccacctgctt atttcgtacc tttttcctct ccgtacatct gcagaacggt atccatccgc
     9001 gccataaaac tggcggcatc cgttgccccc tgcaaaagcg gtaatgcctc aagtgtgttg
     9061 acgttgacca ggaaggtagt cggtgtggcc accgggatat tcgggaagaa ggtctgcatc
     9121 acgtccggtg gcaccggtaa tgcttccgga aaggcggtat ccccctgacc atccagggtg
     9181 tagggaaaaa cggaaaagcc gtactgctgt gccagctgtt tcagtaccgg gtcaaactgg
     9241 tgacagtaag ggcaatgccc ctgcataaac agcaccacct tccagtcggc caggttaacc
     9301 tgcctgccgt tgctgaggcg gaaccagcgg ggagccggct tttctgttct ggctgatgtg
     9361 tctgctgccg gttgtgcagg ctgcacggcc ataccctgcg gattccagag tcgttcaatt
     9421 tcatcccggg tggaggcctg cacacctgtg gccatcatca acaaaatgac cggcagtaat
     9481 ttagtgagag acatgtccgc cctcctgctg cccttccatc atggcctgtt gcctggcttc
     9541 cagtgcctca cgctcttcca ggctgacacg tgcaatccat gcatccagca cctggtatgc
     9601 gccgaacagc acaaggatgg ccgcaatcaa ctccgccaga aaaaaagcca tccagggttt
     9661 gctgtaaaca agacgggcga cgatatgaaa ccagacaccc agggaaaata cccacattcc
     9721 cgtaatatca agccggggta aagagaatct gtatttactt atcatgttaa actccggtta
     9781 ttatttacat gagagtattc atgtctgtaa tattcgcgtt tgtgtgatat taatcagatc
     9841 aacctcatta ttttgtatgg tttttattac cgttttatat gacttagcct gtcataagcc
     9901 cccttataaa aaaccacatc actccggacc atgctgatga caacataata caaacaaaca
     9961 tccttctgaa actgtcaaac ttcgtgataa ccggtatggt actgactgcc attgaaacaa
    10021 ccggtacggc acactgattc ataaccgcac cacaaagaaa caagaaatta tctggcagca
    10081 tacatacact tatgacattt actacaaaga agacaaacga gctcagcaga taaactcctt
    10141 tattatttgg tcctgtaaag aaataaccaa caacccccac gataaacagg ctggcacctg
    10201 taaaaaaaca caaatcccct gtatgcatat ataacctttg gttaaaaatt cggtttaaaa
    10261 tcttcagaaa cgttcaggaa ctgttttgcc aggtcgtcct gtgaaataaa gccgtatgat
    10321 aacgggcgaa cactgccctg tttcgggtca accagcatca tggccgggaa atatttcaca
    10381 ccgaggcgct gcgcctgccc ctggtcagtc cgggaatccg gcaacagcgg attaatcacg
    10441 ccatccacgg aaacgggaat aacactcaga ccataggtat ccctgaagcc attaatgacc
    10501 tgcgccagtt gcccgtcgat ggggtcctgc ccccggtaaa agaacatgat gccgtagtgt
    10561 tcagccagtt tcgcaatggc ctgtcgctgc tgcgcctggt ctgccgccag ctggttccgt
    10621 accgtgccgt tgtaatggct gtactgcagg ttatagtcca gttcgggatg tgccagcatg
    10681 gcctttttag cactcatggt gaaaagcccg gcctgctgag tccagtaatt ctgcagccgg
    10741 aaatatttaa caaaattatc cacgccggga tacagaatgg cttcgtacag cgcccgcttt
    10801 gttgccgtct gcagcgcggc cagtttctgc ataatatccg gctcctgacg gggggctgca
    10861 ggtacaggtt tattttcttt ttccttcgga tttattttct cgttatacca ctgccagcct
    10921 gcatctttcc ctgacgccgg aaaaataaag cagcagagta acagtggcag taatgcttta
    10981 ttcatcttca ttcctttcat cattttttct ttcatcatct ttctgcaggc cagtcagggc
    11041 agaaaaacaa aaatacagaa taaacaggag cacaccggag taaaggataa cgaaagcagc
    11101 ggcaagcatc ccgtctgccg tggtttcttt taccgtatcc cacgaccaga taattaccgg
    11161 ggaaatgaca atcgctgtaa tcgaaagccg gataagaaaa tcaacaagac gattagaagt
    11221 gaatataact ttcatcattt cccccggtta ttttccatac cttactgtcc tgcctgtttc
    11281 agctggtcag caatctgctc tttcactttc tgcgtcagaa cgccactgtc cgggattttc
    11341 tggttattca tcaggtcttc gtagaagtta gtgaaatcca gtctgtcgaa ctgaattttc
    11401 tgcagctcat caaccgtaat cccccgacag tcaggatgct tcgcactgcc aaacccgata
    11461 cgcaactgcc cgttacgccc ctgctgctgg actatctgcg ccagcttcga atcaaactgg
    11521 cagtagctgc gctttttctg cagacagaca cccagcactt tcttcgaaca gaactctccg
    11581 acactgacgg tcagtttgtt gtcctttgcc tttcccagcg ccttttcttc gctgttgcac
    11641 ttcgccagcc ccacatcctg accccagcca ccatccttac agcagttact gaagccggct
    11701 gcggccttac ggcagaattt cgcctcaccc gtgaaagccc tgacatccac accattcagg
    11761 gcggcaacat ccttacctgc ggcggcaagc gcagccagct gtgatactgc ttcaccaaaa
    11821 tcactgcttt ttccgctctg tgccttatcg cattcaccgt caaggcagaa gacatcacca
    11881 ccacagacca tcacttttcc tgatgttctg gactcgcagg agtacgtggc gtactcatgc
    11941 agacaggttc cctcatcgga ataaaacgcg cactgacgtg aggccagcgt acaggccgga
    12001 ttatcgacat atttctgaca ggtgccgtta tctgcggact gagtgacata tttatcccgg
    12061 tatgcccagc atgcctgtgt gatgttccag ggtttacctt ccatcactcc ggttttcgta
    12121 ccacccggct ctgaacactc agtgcctgtc agcacgccat ccgctttatt aaacggacaa
    12181 ctctccaccc actccacccg gggaacccat tctttatcac ggaccttcat cctgagtttc
    12241 agcgtaaagg tggaagcacc actgacaagc gattcataga ctttcctgtc accattcccg
    12301 tgcgggaggc aattaccgtt agcagtacag ccactaccgg tcagaacctg cccctgtgtc
    12361 acctgcaaac ctgtcgcccc ggacaggggg taagtatccg actgcccgtt tgccaggccg
    12421 acattaaagg ttgtattcat gaaggtataa cgggagttca ggaagaaaaa agcggcgtat
    12481 acagacaatg atgcactttc cactgtaccg gttaccggag ccgtgacact gaacaccagt
    12541 ttcagcccgt tcatactgaa ccggaactgt gagtgcggta tcgtcacttc acgatattca
    12601 tccgtgtatt tccagtcccc ggtgatactg gcagtccgtg tgcagtactc ctccaccatc
    12661 gtgtcccgct cacaggtata gtttgtgaat tcactgcggt ttatctcctg cgcactgcac
    12721 tgctgtccgg tatttcccac aatgctgtca gcccggttca ccacatccct gccggtctga
    12781 atgaacggtg catccggtga cagaatgtct ttcggcttgt tcatgaagga ctctgtgatg
    12841 gtttttccgg tttcaccggt cgcccattcc gtggtgccgt catttttcag gccgccatcc
    12901 ccgccggcag tcacgccacc gtaatatttt gtctcgtccg gattcgcgtt atagctgggg
    12961 atactctcct gtggcttaaa gccctgaata ctgcctgtac cctgcccctg aatctgacgg
    13021 gcaaaatcag aaccggcccg gtaatcactg ttgctgtcag cctgtgccat gccggcaacc
    13081 agagccagta tcagaggtaa aatacgtttc atttcccgga atctcctttc cctgccagta
    13141 aatcatgagc cacctgccgg cagtcacccg tggcggccac tttctccagc gcctggccga
    13201 cccgcaggtt tccccggatg atgtcgtatc cctgactgca gaacaccacc agtgccggta
    13261 cactgcgaat gccgtactgt gaaaacagcg tcgggtcgat ctgaacacca tcagtcgcgc
    13321 cgtctttcac cagggacagc acggcttcag cggtggtctt caggtcattg ttcaccatgc
    13381 cccgcagtgt ggcgggtata ccgtagtgcc gggtttcgcc cagcatccgt ttcagcccct
    13441 cttcgggaat ggaaaacgac acaaaataca gggcaccctg ccggggtttc ccgtcctgcg
    13501 aagcctgctg tttacgaacc agctcatcca gaaaatggtt atctgaacgc tgaagggggt
    13561 tttccagcac ctgtttctcc gcccaggctt tcagctgatg gtcaggtttc tctcgcagtt
    13621 gtctgcttaa attttcctgc tgcttcagga actggcggtt ttcagttgtg ttcacatttt
    13681 ctgaagcatg aacagcaaga tgcatccctg ctgtcagagc aaaaagtgca aatattttcc
    13741 ggttcatgat atttctcact ttctgctatt tacaggcatg aactcatcag aaaaaagata
    13801 attgagaaaa acagccacaa acgcacatgt aaatgtcacc ttcagatata atctgacaag
    13861 tgtcacttgt tctgatatat ctgcgttctt ccattcatga ataatttcag cgagaaaact
    13921 gtcaggtgca aaataaaata atataacggg cactattaca agaccaaaag gaaataattt
    13981 taatgttctg cttatcagcc tgagcagtac agcacttatt ttcctgaggt gctgaatata
    14041 tttactgtcc attgttgcca ttttcatttc ctcttattca ttaaaatgat ttacaggaat
    14101 acacagttac gtttacgcca catcaggtag ccgaagtttt tctttgtgtt gggcggattt
    14161 tttcccgtct cccagcgcat cacactgcgc ccgaacggat ggcactgtcc gctgtccgga
    14221 tacatattca ccatctgata acgccagcgt tctttaggca ggattgggga cggatattca
    14281 ttacagacgg cgttattttt cccgatggtt tccataatca tgccctgacg gtgtagtttg
    14341 aacgccatgc gttcactgac cagcagggag gactgcaacg gactggactc attactcacc
    14401 cagccattga acgggtacat actgccctgc gaaccggcac accagaacag aacatcgaga
    14461 ggcatattaa aggcgctggc aatggcatct gctgcacagg ccccctgtgc tatcggattg
    14521 gcaaagatga cagcttccgg attgagaatg gtggtcaggc tgctgtccgt ccaggtgggg
    14581 tcaatttcag aaagataagc gatatccagg tcaccacctt ccagacagcc cagcgatgtg
    14641 atgatgttaa gccagtacgt cagcgggtat ttgtaccagt gaacgtgata gaacgccccg
    14701 ttcacctgct tctcatcctt tttcgccgtt ccctgtgccg ttttaccaaa agccggcagg
    14761 ctgaagccca ggttcaccat gcatcccggt gaccgggtga cgtccgtcag cgccatcggc
    14821 tcccagtaac caatggccag cccgatacgc ctgaacagcg gcggcggtgc cggacaaatc
    14881 tgaatgggca tcgacgggtt cgccgtgtcg gggaccttac cctgactgac tttgatactg
    14941 cccagcgaga gcgggaaaat acagctccag cagatatccg tgatcgggtt tacaaaacgc
    15001 ccctcacagg cagaatccgc agatgccgca gggacatgac cggcgagaag gaataacatc
    15061 agcagccaca ggcttcgctt cattttctgc cctcctctgc cggaataaat tccaccttca
    15121 ggaagcgatc gccgggaacg gcgctcaccc gtgccgggac ctgatcaatg ccgaggcgct
    15181 ggcagagcac accgttctgg tcaaagtaaa cacggctgtc cagagacttc tgcatctccg
    15241 ggatactgcc ctgcacgagg ataattttgc tctccagtgt gggcggcgtc tggcgtttca
    15301 tccaggccac ctgcgccgga tcatcgccat tgatgaaata cagcgtctgg ttaaaaggca
    15361 catactgcag cggattcatc acttcaccct gacgggcaaa cacccgccct tcgttatccc
    15421 ggatatccgc agccagtctg acggacgggt caaacaaccg gctgctgtat ttctccgtgc
    15481 gtccgatacc gggcacggca gggggccgca gactgttgcg gatcacccgc tccttaaagg
    15541 catccatttt ccggcccatc tcaccggact gctccagtgc cgtcaggcgc tgcatgatca
    15601 ccgtcagcat gtccggctcc tttaccggcc acagatcgcc ccaggtgcca agatcggcgg
    15661 cggccacact ctgcccccat atcagcaggg caatcagccc ccggcatctc atggttccgc
    15721 tctcattcgc cgggcaatat cctgctggat ttcccgggtg atatccggag ccccctgtac
    15781 cacggcaggc gacaccagga tgaccgcgtg gtgtttctgc tgccacgcct gcaggctggc
    15841 ttccagtgcc gtgttaaaac gcgctgaaag ggcttttgac tgtgcttctg acagttgttt
    15901 ctgactggcg ctgtcaaaaa acgcatccac ggtctgtttc atgttgaagg cggcagtcac
    15961 cggtgcgttc agtctgacaa tgccgtaact gacggctgcg ttcagtaaca ccattgccag
    16021 gcatcccggc accgtccacc accagtggct gcgtcggggg gctgtaacat ccgtcgtttt
    16081 ttgcgttgtg gtcatgcaac actcctgtat ttctcatgtt cttccagcca ggcttccagc
    16141 gaagccatct ccggccctga cttcttccac gccagctgcc acaccgcctc atgaatactc
    16201 agcccctctt tacgtttctg ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg
    16261 gcgcggctta acgggtccac aaacaggcgg tgccaggagg aatggttttc cacctgcagc
    16321 aggaaggaac tgaaccactg gtctttggcc gcaccaaact taccaatcat gtcgcgctgc
    16381 agtggctgga actggtccgg atacagctgg ttgtattttg cgaactcctt cgcactctgt
    16441 ttgaggataa ttttgtagga ggagttaccc catgccgcac gggccgcact ggaggccttg
    16501 tcagagtcaa agtcgacgat gttctgtgtg atggtgatat aggcaccggt atgacggcgg
    16561 gcggtacggt aacctttctc aataaactca ccgaccttgc gatttttgaa gtccagcaga
    16621 cgccagcctt catcaataac gttcagtttc ttgaggttac gcggcgtgcg gtacatcctg
    16681 ttctcgatgt agataatcag ggagaacatc accgcaacca gcagtgacgg acggtcttcc
    16741 agtccgccca gctccagcac caccattttc gcatcatccc gcagggacgg ctcatcggag
    16801 ttaaaatact ggccgtaagt gccgttggca gtgtactggt caagcagcac aatcatttcg
    16861 tccagacggc tgcggatggt cggtgacccg gcatactgct cgctgtcact ggcgtttttc
    16921 aggaaatcca ccacgtcatc aatacgtgcc ctgttctctt tggccagcca ggaggccctg
    16981 accgcctgca gcagcagacc ttcatgcact tcatccaggt taccgttggg gctggccatc
    17041 actgacaact ggtcacggac acgctccgct gactggtcaa tatcggtgat gttcgcaaac
    17101 gggttaaaac gcagggtttc accgtccaga tatacgccac ccatgttctc acacagggac
    17161 ttgtagccat cccccatgtc gaacaccacg gcaaagcccc cggagtccag cacgctgcgg
    17221 ataagtggct gtatcagccc ggttttaccg gccccggagg tgccacagac cgccatgttg
    17281 tagttggtgt tattcatccc ccggaagaaa atatcgataa acgccagctg gttacggtag
    17341 gtgggtgcca gcagacctgc cggtgtcagg gggttatccg ccactaacgg catcaggttg
    17401 gcaacattaa agctctctgc gcgctgaacc actccggcct ctttcagctg tttaaacaac
    17461 cctttcccgg ccataaaggg cagacaggtc aggaaattgc gcatgtggtt aaagcgcggt
    17521 gaaatcagct caaaaccgtt tttacggaag ctgttcagga tgtcctgctc cacttccagc
    17581 gccgtttcat tgttgtcctt gcagaatgct gtgatgttga ggaagtagga cacgacagaa
    17641 gactgaccgg agcccagacg ctgacgcagt tccccccact ccttcgcctc tttctccacg
    17701 gacggaaacc atttggcata ggaggtcttc gactttttct ccaggtccat gtacttcagg
    17761 ttggcttcgc tgtgggtttt cacctggtct tccaccacca gagtcagcgt caggatgaac
    17821 ggacaggaga tggacagttc cgggttcagc aggttgctgt agttgtcggc catattccac
    17881 aggaaggcga tttccgggtt acgggccaga tggaaattca ggatacgggc cgtgctgttc
    17941 ctgccgttct cacgcaggcc cagcgtcagg taatcagccc ggacattcag gtcaaaactg
    18001 tcctccacac actgataatt cagatcagaa tacgggtcca gctgacgtct tttcgggtac
    18061 agggagtccg ggttatggtt aatcatctcc ccgacaatat cgataaaggc ctgtgcatcc
    18121 accgtctgtg tggcgatact ggccccctgt aacgacgccc ggatgatttt caccaggttt
    18181 tccatttcca gaatgtcagc ccggcttttt ttcttcgagg gagaacagta cgagataaat
    18241 acccggtaat ggcgcagggt caggggcaga ttcatcccct ccggcagcgg aaactgtgtc
    18301 gctgccgctt tcatataata ggcccgggta atggcgttaa accgttcagc ctgttcacct
    18361 gaccaggaga actcacgcag cccgtactca atcctgtcac caaccagctg actggacatc
    18421 agatggatac acaacggaat accgcgcggc agtttggtgc gcagcatgtg atcgagtgcc
    18481 tccacaatgg acttattcgc cccattgatg ggaatggctt ccagcataaa gcccatcgtg
    18541 gtgtcattca tgaacagacc ggattcctgg ttgtaatcac ggtacggcag cagacggctg
    18601 aactgcggga agctcatttc gcccagaact tcattggcct ttgcggattc gtcaggcagt
    18661 ttcagtgctg tgacgaggga gttaaccgcc tgagtgacgg cctcaagtgg gttattactc
    18721 atatgagtat tccttagtgc gtttaaaaaa ggaaaatatc agtctgtggc cgtggatata
    18781 tcacgcagtg ccattcctga aaccacagca caaataagag atggaggctt ttaacaggaa
    18841 accaggatta acgttccttt actgaatgag catcatttaa tgcctgaatt tgtttttcgc
    18901 gaatcttatt gcgctcattt tctcttacac gtttcacctc tgccatttcg tttctttttc
    18961 tgcattctga tgcgatttta tttgccttac caagtgttaa aggttcagac tgataattaa
    19021 gtgtctcctg aaatgcttct gctgcacagg gagtttcctg taaaatctct gtcagttgtt
    19081 ctggtgttgc aggttgttgc gcaaggtaaa cgatgtaaaa aggaatacat acaatcatag
    19141 ccataacgcc aacaaatata aaagccagaa cagattttaa atatatcgac ttcatttcat
    19201 tttccttcga agcagtaagc actcattgcc caccaccatt tagtgtttcc atcacgctgt
    19261 tcaaaggttc atttgtttta acttcaatac actcccctgt atttccaaag cccctgccgc
    19321 catgtgcttc gattgtccga agaacctgca cgcatacgtc cgtatttggc atgggtgtaa
    19381 tattccagct tccgccaccg ttaatatttt ttatcagcac aagaaaaata aaggtatgca
    19441 ttctttatta tctccgcttg tatattaatc actgaatatc ccttcagtta acttatgagg
    19501 tcataaaaca ctgcagattc tcattcggat ttattccctt cagtaaactt ccgaatagta
    19561 tcctgttcac gctgacgggt ggcagactcc tgcattgcat ccactttctc ctgccagaga
    19621 cgccggatat cctgttcatc ccgaccagtc agttttttac cggacaacaa acggttaagc
    19681 agttcctgtc tcacatctgg tacgtcttca gtcagacgca taagccggag taattcctga
    19741 cgactgagta ccatgcgtga ttcctccggt acgctgtaat aattaaaaat acgactgaat
    19801 accccacaac agcaggcaac ggacaggcac agggatttca gatacaggcc agaaacacag
    19861 aatgtggcaa caccacaaag aatagctata actaaaaatc ccttacccag tgccatacac
    19921 aaagaaccct gttttctgga tatccgctca tgctcatcca gaataaaatc tattttttca
    19981 ttttctgatt tatgcatagt gtttcctctg cctttcctga tacgcctgac attcaacgca
    20041 caacgtcaca ccgggaaata ttttccgccg ggcttccgga ataggatttc cgcatgcttc
    20101 acagagataa acaggaatac catgaacatt tatttcctgg cgtatccggt ttattcctgt
    20161 catggtcagt tgttctgtca ctgaatatgc ttcatcggct tcatcactca cggaatattc
    20221 ctcccgacat gttctgacac gctaccgccc atcccttttc acgcacaggg cactcgtttt
    20281 acaggcggat tttgaataca gaaagcccgc cggcattgcc ggctgatata caacttcagg
    20341 gccagttaat taatacgtgg ttttccccac gcagacggtt taataacaaa aaacacgctg
    20401 gatggctgat gataaacatc ctggttatca atataaggcg ctatccataa tgctgccgtc
    20461 tgctcacccg ttcttagcgg acgaggaggt gttaccggcg taactgaact gaccggaaca
    20521 accgttttca cttcccgggc agcggtaaac agcgggcgtg gtgccagcag tttctgctcc
    20581 ggatgtgctg tcaccgcagg tctgctgccg gacggagtgg tcgcggtgac ggtctgcacc
    20641 ggcattgtcc ggaagttccc ttcagccagg cgcggcagtg atgccgcaac cggctttgct
    20701 tctgaggact gctccagttt tttggccttc tcattggcct gctccatcgt catacaggta
    20761 tcggatgtgg tggcgttaca ctcaaattcc gtatttgttc ctgcgcagcc ggaaagtaat
    20821 aaggttccca gcagaggaat aaataaagaa atctgtttca ttgcccttct ccttttttat
    20881 tactccccac aacagcagaa attaactctg ctgtcagatt tgttttccct gcacagggat
    20941 gtccggagac cactgtattt cccggagaag aaaataagct catcgacatt cccttcttca
    21001 tcacaccgac agcagccata ttaaaattgt gcggcaatga ttcagaactg aacaccttcg
    21061 gagcaggaag aactattttt gtggtgcagt tactgagaac agcatgcata aaatcatctg
    21121 atacgttttt atccggcaca acgcgggctg taataacatt aatattacgc atgttcattt
    21181 ttatctccgt tgcttctgtt tgttcggtca tcagggaaat aaacatggca tttcctgatt
    21241 tttcccggca gggaagcgtc ttccgtataa acgtccttca gatattcatc tgcggttacc
    21301 acccggatac atttcatttt tccgggcagc accggatatt tcccggtgcc tgccgcaaac
    21361 gacatataca tcagtgccag cgttgcccag aatgcggtga ccagttgcat cagtgaccgg
    21421 tcagtctccc tgacataatc tgcagcatca gccgcgcggt aatggcagat atcctgtatt
    21481 tctgatctgc ctgaagaatc agaggaagaa aaccagcatt cactgaccgg gaactgtccg
    21541 gcaggtgctg tttgtcttac gaagttaatc tccttaacca gtaatttccc cggcgtggtg
    21601 ttgtccttcc ataaaacaaa cagagccatc agggccattg tggccagcgg ccagataacc
    21661 acaaacctga cgacccacag cagccagcga acgacaaacg ccacacggaa tgtcagtgca
    21721 tcacccgcca tttccgccct ccttgccggc atccccgccc agcgggttct tgtctccgga
    21781 cataaactgc tgcggattca gattgcccag ctgtttcagc atctggtcgg tgttaaaacc
    21841 gttcaggtgg ctttctgccg agggaggatt cggcaccggg ctttccgggt taccctcttc
    21901 cgctgtactc tgtgactgct ccagtgccat ttcctccacg gttttcagct ggaagccgtc
    21961 ctggaacacc acggtcactt cgttgcccgc accaatcggt atcaccgggt gatactgttc
    22021 ggcacgtttg atgtagtagt cactgagcgt ctgtgcggct ttcgatgcgc cgccaccgat
    22081 acccattttc aggacatcac cagccccgta agcggctgtg gcacccagcc cgacagccgg
    22141 ttgggaggcg cgctccattc cctgaccgat accgtcaaca aatcccgcgc cccatgccca
    22201 gccgaggatt ttgccgttac gcatcaccac ttcgcccttg ataccgtttt taccccggaa
    22261 gctgacatgc cccttaatcg gcatatcaat agttttgccg tccttcaggc aactgatatt
    22321 gcgggtgcgt acaatggcac gttcactgga cacatccccc caggcttcca gccccacaaa
    22381 acatcccgtt gcgtcatacg tcttgctgtt cggcatttcc accaggccgg tgatacgcaa
    22441 ctgcatcggc accgtggatt cattaccggt gactgaggca ttggcatccg ccccttcaat
    22501 cagcatggct ttcgcaaaac ttcctgacgg aatgtacggc agcgatggtc cctgttttcc
    22561 ctcattacgg gtaaacacct tacgctgtat ccggttaggc accggcacag actggtacgt
    22621 cacctgtggt ggtggcgtga caccattccc gggataaaac gccgtcggcg gtggaacagc
    22681 aacgctgccc tgcggcggga aggatacggg ggtgtttcct ggctgtggtt cgccttccgg
    22741 gccgggcggt ggggcaggca tctgcggtac aggctcaccg gtcgccgtga cgggattagc
    22801 acccagggct tttacctgct ctgccagggc ggcattgtcc tgtcccagct tttcaatacg
    22861 acgctggtca tcaccgcgct gtttgttcag aacatccagc tcacgacgga tttcctcata
    22921 ctgcttctgc atctgcgctg ccgtcacctg catctctgtg gtggcatgct gacgcacctt
    22981 gtcatcaaag gtcgtatcca ccacaccggt catatccggc acaggctcct gttcagccac
    23041 ggtttcaccg ttaccggaca tgtccacatc agacagatac agtgccccac caatcgcgga
    23101 ggctgtaccg acaaccacaa tccccagcca caggtactgc ttgcgtttca caatggtatt
    23161 gatactggcc atcagttgcc ctccccgtca cggatgacat acacatccat gcgcgcacct
    23221 gccagtaact ggctggcagg ctggctgaac atcacggcac gggttcccgg ctgccagaaa
    23281 tcactttccc ggatattcag ggcagacagc gttttatttt ccacgacaaa gcggaccatc
    23341 ttcaggtgat tccccgtcca tacggaatct gccactgaag acagccccgc cggggcctgc
    23401 agggtttcct ttgtcacagg aacctgatac cagcctgcag gtaattttcc gccacggaca
    23461 gcctggctga tggtcacaag cagggattcg taaggcgtgg acgtttccca cgctccggct
    23521 tcttctccgg ttccgcgcag gtcactgacc agctgaatgg tacgccccgc gccttcacgg
    23581 ggaacagcct gaatggaaag gttcagacca cgctctgttt ccagaatgaa cgtaaagggc
    23641 tttttgttga cggtggccac caccacaccg ccggaggcgg tctgctcatt attggtcagg
    23701 gcaccatcca gactgttcac ggcgataatc cggtcaccgg gaacggtaaa cagattcggg
    23761 ctggtattgc tgatggcaat actggcctga ccaccgttca ccattggcac cacagtgggg
    23821 gccagcgtac cgtttgcggc catcacgctg caggaaaaaa acaggccgcc gaatattatt
    23881 gccgtattat tttttctcat cgtctgtttc cccgaaatta tccagccagg tcaccccgtt
    23941 ttcccgcttc agaataagga tgtaatgttt gatatcagta aaaggttcgg agttaccaat
    24001 ccatgttttc cgaattccac ggatttccac acgaccatac tgaggccaga cacggacact
    24061 ggtctggaaa aaagcagaat tcacgttatc ttttttaatg agtttcgctt cttcagccag
    24121 aataactttc atctggttct gtgcacccgg gcggatatat tgcagaagcg cctgatgtga
    24181 agcatcgacg atttccggtg agacattcag acggagggca ataaatgaca gcgccatctg
    24241 ctgcaaataa gaggcatcgg cactgttctg tgacacggca aagggcacat taaatcccat
    24301 tggggtcacg gcagtgcgtt gctcattctg aagacggtag ttattcaccc cctgaatgag
    24361 gttaacagag aggctgagaa caataagcac tgacataaat ataaaggcga tggccattac
    24421 acgactggta cttaaacggg caccgtgttc catataacaa tcctggtatc agttctattt
    24481 aatccactgc cggaaacacg aatcgggaac attatgaaaa ataccgcgca gcagggctgt
    24541 tggcatatac cagtaaatca ggtcacgtaa ccaggaactg ccccgccctt ttttcagttt
    24601 tttaatcccg aaataaacca gaaccgctgc accaataccg aacagatatt tcgatgttgt
    24661 gataccccag ccaacacaga ttgctgcggg gatcagttca tccagtggca ggccaaacca
    24721 gcggctctgg
//
LOCUS       DAZFKM010000069        24482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFKM010000069 DAZFKM010000000
VERSION     DAZFKM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..363
                     /locus_tag="ACHKAY_002858"
     CDS             <1..363
                     /locus_tag="ACHKAY_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO5701517.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHKAY_002859"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHKAY_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO5701518.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHKAY_002860"
     CDS             2185..3504
                     /locus_tag="ACHKAY_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO5701519.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5263
                     /gene="dosC"
                     /locus_tag="ACHKAY_002861"
     CDS             3881..5263
                     /gene="dosC"
                     /locus_tag="ACHKAY_002861"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO5701520.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5288..7687
                     /gene="dosP"
                     /locus_tag="ACHKAY_002862"
                     /gene_synonym="pdeO"
     CDS             5288..7687
                     /gene="dosP"
                     /locus_tag="ACHKAY_002862"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO5701521.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHKAY_002863"
     CDS             7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHKAY_002863"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO5701522.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8540..10090
                     /locus_tag="ACHKAY_002864"
     CDS             8540..10090
                     /locus_tag="ACHKAY_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO5701523.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10092..11114
                     /locus_tag="ACHKAY_002865"
     CDS             10092..11114
                     /locus_tag="ACHKAY_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5701524.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHKAY_002866"
     CDS             11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHKAY_002866"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO5701525.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12004..12990
                     /locus_tag="ACHKAY_002867"
     CDS             12004..12990
                     /locus_tag="ACHKAY_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5701526.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12983..13909
                     /locus_tag="ACHKAY_002868"
     CDS             12983..13909
                     /locus_tag="ACHKAY_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO5701527.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHKAY_002869"
     CDS             complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHKAY_002869"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO5701528.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14742..14957
                     /gene="bdm"
                     /locus_tag="ACHKAY_002870"
     CDS             14742..14957
                     /gene="bdm"
                     /locus_tag="ACHKAY_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO5701529.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15059..15196
                     /gene="sra"
                     /locus_tag="ACHKAY_002871"
     CDS             15059..15196
                     /gene="sra"
                     /locus_tag="ACHKAY_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO5701530.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15353..17050
                     /gene="maeA"
                     /locus_tag="ACHKAY_002872"
     CDS             15353..17050
                     /gene="maeA"
                     /locus_tag="ACHKAY_002872"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO5701531.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17184..18194
                     /gene="adhP"
                     /locus_tag="ACHKAY_002873"
     CDS             17184..18194
                     /gene="adhP"
                     /locus_tag="ACHKAY_002873"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO5701532.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18341..18625
                     /locus_tag="ACHKAY_002874"
     CDS             18341..18625
                     /locus_tag="ACHKAY_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO5701533.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHKAY_002875"
     CDS             complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHKAY_002875"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO5701534.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHKAY_002876"
     CDS             complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHKAY_002876"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO5701535.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHKAY_002877"
     CDS             complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHKAY_002877"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22679..22681),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5701536.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23498..24379
                     /gene="yddG"
                     /locus_tag="ACHKAY_002878"
     CDS             23498..24379
                     /gene="yddG"
                     /locus_tag="ACHKAY_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO5701537.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5992 a         6211 c         6190 g         6089 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctaaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctaca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttattttagt ggcattgccg aagtaggcca tatctcccgt ctgattcagg atttcgacgg
     4621 tatattcaat caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa
     4681 atttttatta cagataagaa atactgtatc gcaaattatt accttgctgc gtgaattgtt
     4741 tgaagaagta tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg
     4801 tttcctgccg actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccact
     4861 gtcagtgctg attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa
     4921 cactggcgat gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccgcagtag
     4981 tgattatgtt tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga
     5041 aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa
     5101 agccgcaaac ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg
     5161 tcatcctgac tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag
     5221 acgaggtaga aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga
     5281 ggtaatcatg aagctaaccg atgcggataa tgccgccgat ggcatttttt tccccgccct
     5341 tgagcaaaat atgatgggtg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa
     5401 ccccgccgca gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga
     5461 tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga
     5521 aggcggtaaa gcgcgtgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg
     5581 cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta
     5641 cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga ctcgacaatt
     5701 gattattgcc gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat
     5761 tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg
     5821 tatgcagccc gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt
     5881 acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac
     5941 cggtgaaaaa atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct
     6001 gcagaacctg gtaatgactt tctcggatat caccgaagaa cggcaaatcc gccagcttga
     6061 aggcaatatt ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat
     6121 ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa
     6181 cgggatgccg atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag
     6241 ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg gggatcctgc aaattaaaac
     6301 ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gtcagcatat
     6361 ggccgcgctg gcgctggaac aggaaaaaag ccgccagcat attgaacaac tcatccaatt
     6421 tgatccgatg accggtctgc caaatcgcaa taacctgcac aattacctcg atgacctggt
     6481 cgacaaagcc gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat
     6541 tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg
     6601 tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt
     6661 gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac ggaatgtggt
     6721 cagcaagccg ataatgattg acgataaacc attcccgctt accttgagta ttggcatcag
     6781 ctacgacgtg ggtaaaaacc gcgattacct actctccact gctcacaatg caatggatta
     6841 tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt
     6901 aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa
     6961 actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct
     7021 tgctcgctgg cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc
     7081 agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca
     7141 gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc
     7201 gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg
     7261 tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac
     7321 cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggcttat cggtagatga
     7381 ttttggtacg ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa
     7441 aattgacaaa agttttgtcg atcgttgtct gaccgaaaaa cgcatccttg ccttacttga
     7501 agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac
     7561 caaagagcaa tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt
     7621 ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt taccgctgaa
     7681 aatctgacaa attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact
     7741 taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca
     7801 ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc
     7861 agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat
     7921 ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgta
     7981 atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa
     8041 gctatttatc agcaagcgcg ttgtctgcta cacaaggatg cgattaccgc gctggcgaaa
     8101 agtatcagta tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca
     8161 caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg
     8221 acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac
     8281 gggaacatcc tcgatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat
     8341 cacccttccg tcccgcccgc cgctcagcgc aatcggctgt tgctgaatgc gataatgact
     8401 ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg
     8461 agttaccctc tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc
     8521 agttaatcag gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct
     8581 cgtccttgcc acaaatttcc cggtttcgca cgccgccgta ccgaaagata tgctggtgat
     8641 tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg
     8701 gacggtgacc tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg
     8761 ctcaaccgac gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga
     8821 gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg tcactgccga
     8881 agcagtaaaa ctttcttttg agcggctact aaaaatcggc caggggccag cagaagcatt
     8941 tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta cccttagcca
     9001 accattcgca ccgttcctct acacgctggc gaatgacggt gcatccatta tcaatccggc
     9061 ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg
     9121 ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc tggtgccaaa
     9181 tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga
     9241 aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc
     9301 ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc
     9361 actgcgcgtt acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct
     9421 acgtcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg
     9481 taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc
     9541 aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa
     9601 acccaccagc ctgacgtttc tctactccga taacgatccg aactgggagc ctattgcttt
     9661 ggcgacacaa tccagtctca acaagctggg tatcaatgtg aagctggaaa agctggcgaa
     9721 cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag
     9781 tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg
     9841 tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc
     9901 gcttgcgacc accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt
     9961 cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa
    10021 agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat
    10081 gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg
    10141 tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg
    10201 cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac
    10261 tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac ctgtttcatg
    10321 gtgacctggg aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt
    10381 tcccggcgac gctggagctg gcttttggtg ccctgctgct ggcactcctg attggcatcc
    10441 cgctgggcat actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa
    10501 tggccattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc attgtgctgt
    10561 tttatggtca tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac
    10621 caacgcacgt taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct
    10681 tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg
    10741 gaattgtcgc tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc
    10801 gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca
    10861 atgcgttgat cccatcgatt accgtattgg gtttggcgct gggcgatttg ttgtatggcg
    10921 cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa
    10981 tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg
    11041 tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg
    11101 gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg
    11161 atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc
    11221 agtcatcatt gtattaatgc tattgatgat gattttttca ccgtggctgg cgacgcatga
    11281 tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg
    11341 caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat
    11401 tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct
    11461 atccggtgtg cttggcggac gcgcagacgc catcatcatg cgcatcatgg acattatgct
    11521 gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt
    11581 taacgccatg ctggcgcttg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg
    11641 gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc
    11701 gcgttggcat ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca
    11761 ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct
    11821 gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcgaatggtc gcaactatgt
    11881 actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgttgg
    11941 gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca
    12001 gtcatgaccc aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac
    12061 ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggcga aattgtcggt
    12121 ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta
    12181 cagacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt
    12241 aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag
    12301 gaaccgatga ccgccctcaa tccgacactt cgaataggtc ttcagatgat ggacgtgatc
    12361 cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc cctgctggaa
    12421 gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt
    12481 ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt
    12541 gccgacgaac cgactacggc gctggacgtc acggtgcagt tgcaggtact gcgtttgctt
    12601 aaacataaag cccgcgccag tggaactgcg gttctgttca tcagccatga tatggccgtg
    12661 gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc
    12721 gtgacggcag atgttatcca tcatccccgg catccgtata ccattggttt gctccaatgc
    12781 gcaccagaac atggagtacc acgccagcta ttacccgcca ttccagggac ggtaccaaac
    12841 ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag
    12901 tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg
    12961 tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg acgtccatat
    13021 caatttcccg gcccgtaaaa actggcttgg taaaactacg gaacatgttc atgccattaa
    13081 tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg agtcaggctg
    13141 cggcaaaagc accctcgcac agcttttaat gggcatgctg caaccgagcc acgggcagta
    13201 catccgttct ggctcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct
    13261 caatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag
    13321 tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc
    13381 ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat
    13441 tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct
    13501 ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg
    13561 gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt
    13621 ggcggtgatg tatctcgggc agattgtgga actaggggaa gcgcaacagg tgttgactgt
    13681 tccggcacat ccatataccc gattattgct ggattccctc cccgccattg ataaaccgct
    13741 ggaggaagaa tgggcattac gtaaaacgga tctgccggga aaccgcacgt tgccgcaagg
    13801 ctgttttttc tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt
    13861 aacaataagg gaggacggac gtgagatccg gtgctggcgg gcgctgtaga gatgctaaca
    13921 acgcatcagg cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg
    13981 taatcaagcg taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc
    14041 tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt
    14101 gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc ggcggtggta
    14161 tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa
    14221 catgcggcat gcgctgcgcc aatcagttct tcagggttag ttcctttttc gccttcaaaa
    14281 cgcgtgttaa atccatacgg ctgttggttc agcacaccac tctcggtgga tactgttccc
    14341 ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt
    14401 gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag
    14461 caggatattc cgaataaaaa tccggcagaa atattacttc tgcacttaaa ttaaatttcc
    14521 acaatagata accgggaata tataacccaa gagatattgc attaatatta atcaactcac
    14581 cacaataata atacctatca atatcatgag gttaaaatat aaaaatcatc aaccaggact
    14641 aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata aatctgcgat
    14701 ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact tattatcagg
    14761 cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc cttcgggcag
    14821 agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg gtggaagctt
    14881 ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg gtcagtggcc
    14941 agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg gtttagcacg
    15001 tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag gagttaaaat
    15061 gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt ctaaccagcg
    15121 caaaatagtt accgaaggtg acaaatccag cgtggtaaat aatccaaccg gcagaaaacg
    15181 ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca ccattgcctg
    15241 cgcaatggtg tttttgtttt tatctgctct atacttgggg ccgacgccct ggcggtaaag
    15301 caaagacgat aaaagcgtgc cagggatgga tattcaaaaa agagtgagtg acatggaacc
    15361 aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac tgctggaatt
    15421 tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact tcaacctgct
    15481 ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca
    15541 gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac
    15601 caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat
    15661 ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg
    15721 cggcgtgttt atctcttacc agaaccggca caatatggac gatattctgc aaaacgtgcc
    15781 gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga
    15841 ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg
    15901 cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca
    15961 gctgcttaac gatccgctgt atatgggctg gcgtaatcca cgtatcactg acgatgaata
    16021 ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt
    16081 gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga
    16141 aatttgttct tttaacgatg acattcaggg cactgcggca gtaacagtcg gcacactgat
    16201 cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc
    16261 aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt
    16321 aagcgaggaa gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga
    16381 caagatgccg aacctgctac ctttccagac caaactggtg cagaagcgcg aaaacctcag
    16441 tgactgggat accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc
    16501 agatattctg attggcgtct caggacagac cgggctgttt acggaagaga tcattcgtga
    16561 gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtcacgcgt
    16621 ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg
    16681 cagcccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa
    16741 cgcctttatt ttcccaggca tcgggctggg tgttattgct tccggcgcgt cacgtatcac
    16801 cgatgagatg ctgatgtcag caagtgaaac gcttgctcag tattcgccgc tggtgctgaa
    16861 cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc
    16921 gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt ctgccgaagc
    16981 tctgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac
    17041 ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc tccctttttc
    17101 agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga
    17161 acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat catcatgttg
    17221 acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt
    17281 gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaactg
    17341 gggtaattct gggccatgaa ggcatcggtg tggtggcaga agtgggtcca ggtgtcacct
    17401 cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc ggtcattgcg
    17461 aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg
    17521 ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag
    17581 atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc acctacaaag
    17641 ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc
    17701 tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgccaaagtg atcgccattg
    17761 atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta gcgattaact
    17821 cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg
    17881 tggtaacagc agttgctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg
    17941 gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgccttg
    18001 tgctggatgg cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag
    18061 ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg
    18121 acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg
    18181 atttccgccg ttaagaggcc tttgctgcga gtgccagtcc tggtgtcgca gcatcgcact
    18241 ctctccgaca ttaaatggct aaatggatta ttgttccttc ctacgcaaca ataatgatag
    18301 tgttcctgat gaaggaacac tactgagagt aaagacactg atgaaaatgg ccaatcatcc
    18361 ccgcccgggg gacattattc aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt
    18421 tgccagagca atggaaattg ctccctcaac ggcaagccga ttgctgaccg gaaaagcagc
    18481 tttgacacca gaaatggcga ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg
    18541 gctgaatctg caaaacgcct ggagtctggc agaggcagaa aaaacggtgg atgtgtcgcg
    18601 actacgccgt ctggttacgc aataaaaaaa tggcgcttct taaagcgcca ttttcagctt
    18661 aaagttcaaa agagattata tcccttcttc actctctttt ttcgcttctg ccttctcgat
    18721 ttcacgatac cagcgcggat ggtgtttctt cgcccagcga cggctcacct tcccttcgat
    18781 catcccttta atcgaccctt tcacccagaa tgccatatac atatggatca ggatggcgtg
    18841 gatcaggatg atacccgcag ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg
    18901 gaagtactgc gcaaagtacg gacgccagat aatcaccccg gtcaccagca gcacgaaaat
    18961 catgctcatg atcgaccaga acatcatctt ttgcccggcg ttgtacttac cgacatccgc
    19021 cactttatgc tcattgcctt tcaatacttc gacaatgttc aacagccacg gaatatcttt
    19081 cttatccggg atgttgtgat gcacaaaacg cacaaacata aacatcagtg cgacgaaaat
    19141 cgcaatgccg aaaaacgggt gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt
    19201 cagccattgc agcgtcggga agaagaacga aatcccggac agcgccacca ggaagaagca
    19261 aatcaccacg gtccagtgac aggcgcgatc aataaatttg gtgcgcacaa tcattttcga
    19321 cttactcatg atgatcctcc tcgtcatcgt ccacttcctt attcgggcca ataccgatgt
    19381 agtggaaaat caacccggca aaagtggcaa taaagccagc cgctgccagc ggtttcaacg
    19441 cgcctttcca caggcttacc gaggtgtcga tcttcggatc tttcggcaga ccgtgataca
    19501 gctccggctg atcggcgtga tgcagcacgt acataacgtg cgtaccaccg accccttccg
    19561 ggttgtagac gccagcatgt tcgtaaccac gcgctttcag tttcgccacg cgctgttccg
    19621 ccagctccag catctccttc ttggtgccga agtggatagc cccggtcgga caggttttca
    19681 cacaagccgg ttcctggccg acgctgacgc gatcgacgca gagcgtgcat ttatataccc
    19741 ggttatcctc tttgttgagg cgcggaatat taaacggaca cccggcaatg cagtaaccac
    19801 agccgatgca gttttccgac tggaaatcga caatcccgtt agcgtactga atgattgcac
    19861 cagcagacgg gcacgccttc aggcagccgg gatcttcaca gtgcatacag ccgtctttac
    19921 ggatcagcca ctccagcttg ccgttctgtt cggtttcgct aaagcgcatc accgtccagg
    19981 acttggcgct cagatcggcg gggttatcgt aaaccccgac gcagtgcccc acttcatcac
    20041 ggatgtcgtt ccactccgaa cacgccacct gacaggcttt acagccgata caggtggaaa
    20101 cgtcgataag ttttgcgact tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg
    20161 agttagttgc ggaccttttg ataatgtcct gcgtttccat agccatctgt tcgccccctt
    20221 acgccttctc gatgttgact aagaacgctt tatattccgg cgtttgcgag tttgcatcac
    20281 cgacattcgg cgtcagagtg ttagcgatat aacctttacg cgcgacaccc tcaaagcccc
    20341 agtggattgg aatacccacc gtttcaacct gctgaccatt tacattcagt ggtttcagac
    20401 gacgcgttac cacagccacc gcgcggataa agccacgctt gcttgagaca gtgacacgat
    20461 cgccattatt aatgcctttc gccgccgcca gcgtttcgct gatttccaca aactgttccg
    20521 gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt gtggaagtgc tcggtcagac
    20581 gataggtcgt acccacatac gggaactgct cttttttacc catccgcagt gcgtcttgtt
    20641 catacagacg aacaaccggg ttagacacca cgttcggatg cagcgggtta gtgcccagcg
    20701 gcgtttcaat cggctcgtag tgttccggga acggaccttc cgccattttg ttgatggcaa
    20761 acaggcgtcc catcccttcc ggctgcatga taaacggccc ggttggcgta cccggtgcgg
    20821 cattgccgaa gtcaggaata tcgttacccg tccacttgct gccgttccac tggatcagca
    20881 tccgtttcgg atcccacggt ttaccgttga tatccgccga agcacggttg tacagcacgc
    20941 gacggttgag cggccacgcc caggcccatc ccagcgtatt ccccagaccg gacgggtcgg
    21001 agttatcgcg gttagccatc tggttgccct gctctgtcca gctaccggtg tagatccagc
    21061 aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa gctactcagc aactgacctt
    21121 tcttcgcaat cagcacgcca ttagcatcat agagatcttc cagcgcatag ccgttgttct
    21181 ctttagctac ttcgtcagat tgcggttcgt gcggctgctt gtagttccag ctcatcttca
    21241 tcagcggttc tacgccttta ccaccttcgg cctggtacag ctcgcgcagg tgatggtaga
    21301 tacccgccag aatttcaccg tcgttacgcg cttcgcccgg cgcatcctga cctttccagt
    21361 gccactgcag ccagcgaccg gagttagcaa tagaaccatc ttcttcagca aagcaggtcg
    21421 aaggcagacg gaatacttca gtctgaatag acgccggatc gacatcgttc gactcgccgt
    21481 ggttctgcca gaaggttgag gtttcagtca ccagcggatc gataaccacc atgtacttca
    21541 gcttgcttag gcagctcacc actttgtttt tgtccgggaa ggacgcaacc gggttaaagc
    21601 cctggcagaa ataaccggtg actttgcctt catccatcat gttgaaatac ttgatgacgt
    21661 cgtaggtctg gtcccacttc ggcagccagt catagcccca gttgttctct ttctgcgcgg
    21721 catcgccata gaaagatttc atcaggctaa cgaagaactt cggatagttg ctccagtagt
    21781 tcacctgacc agccagcgtc gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa
    21841 cctgtttttc tgacggcagc gtcagataac ctggcaggct ggtagagagc agacctaagt
    21901 cagtcagacc ctgaatgttg gagtgaccac gcaatgcatt cacgccgcca ccggccatac
    21961 ccatgttacc gagcagcaac tggatcatcg ccatagtacg gatgttctgc gcacccacgg
    22021 tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt gcgatccggt gcgctggtgg
    22081 aggccagcac ttcacacact ttcaggaagt cggcttttgg cgtaccacag atgttttcaa
    22141 caacgtccgg cgtgtagcgg gaaacgtgcg ctttcagcag gttccacaca cagcgcggat
    22201 gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc gaactgatag ttccaggacg
    22261 atttatcgta ttgccgtttt tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa
    22321 aatcatcacg caccagcagg ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt
    22381 tgtcgttttc gatcaggtag cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac
    22441 gaataggtgc gtaaatatcc gccacagaag cggtacgcgt aaaacgagga tcgacaacga
    22501 tcaaggttgc atcgttgttg tttttcgctt ccatcgccca gcggaaaccg acgggatgcg
    22561 cttcagcagc gttaccgccc attaccatta cgacgttagc gtttttgata tccacccagt
    22621 ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc
    22681 agacgcgcgc ctggttgtct accgccagca tcccgaggga gcgggcaaat ttctgtgtca
    22741 gcatcccggt ttcgttgctg gcaccggagg cacacagcat accggtagaa agccaacggt
    22801 ttaccgttac gccctgctcg ttcttttcaa taaagttagc gtcacggtca gctttcatca
    22861 gctttgcaat acgggagaat gcttcttccc agctaatgcg ctgccatttg tcagaacctg
    22921 gcgcacgata ttccgggtag cgcagacggt tttcgctgtt gacgtaatcc agcaaaccgg
    22981 ccccttttgg gcacagcgca ccacggctta ccggatgatc cgggtcacct tcaatgtgat
    23041 aaatcgcttc tctggcgttt ttcgcgccat cacccaggct atacatcaat agcccgcaac
    23101 ctacggaaca gtatgtgcag gtgttacgga tctctttagc gcgtaataat ttgtagtttc
    23161 gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctaccgtt gttccagcca
    23221 taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct ttcctctttt
    23281 ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa
    23341 tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc gcaaattgac ctacctcaat
    23401 agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta taaaattcag
    23461 cttaattttt aacggcaaga gagacaaaac agcgagcatg acacgacaaa aagcaacgct
    23521 catagggcta atagcgatcg tcctgtggag cacgatggta ggattgattc gcggtgtcag
    23581 cgaggggctc ggcccggtcg gcggcgcagc tgctatctat tcattaagcg ggctgctgtt
    23641 aatcttcacg gttggatttc cgcgtattcg gcagattccg aaaggctatt tactcgccgg
    23701 gagtctgtta ttcgtcagct atgaaatctg tctggcgctt tccttagggt atgcggcgac
    23761 ccgtcatcag gcgattgaag tgggtatggt gaactatctg tggcccagcc tgacaattct
    23821 ctttgccatt ctgtttaatg gtcagaaaac caactggctg attgtaccag gattattatt
    23881 agccctggtc ggcgtctgct gggtgttagg cggtgagaat gggttacatt atgatgaaat
    23941 catcaataat atcaccacca gcccattgag ttatttcctg gcgtttattg gtgcgtttat
    24001 ctgggcagcc tattgcacag taacgaataa atacgcacgc ggatttaacg gaattaccgt
    24061 ttttgtcctg ctaacgggag caagtctgtg ggtttactat tttctcacgc cacaaccaga
    24121 aatggtattt agcacgcccg tcatgattaa actcatctct gcggcattta ccttaggatt
    24181 tgcttatgct gcatggaatg tcggtatact gcatggcaat gtcaccataa tggcggtagg
    24241 ctcgtatttt acgcccgtac tttcctcagc gcttgcagcc gtgctgctca gcgccccgct
    24301 gtcgttctca ttctggcaag gcgcgctaat ggtctgcggc ggttccctgc tctgctggct
    24361 ggcgacacgt cgtggttaaa ataattgccg ggtctacggc ccggcagtta atcagataaa
    24421 ttccgacaat attaaaaaat aaatttcaaa taccagtaat tcaccttata tctcatttcg
    24481 ac
//
LOCUS       DAZFKM010000070        24340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFKM010000070 DAZFKM010000000
VERSION     DAZFKM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..358)
                     /locus_tag="ACHKAY_002879"
     CDS             complement(<1..358)
                     /locus_tag="ACHKAY_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701538.1"
                     /translation="MENVRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDII
                     LSLCFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCALIACFIFLFLVLSIN
                     IFSVSASLYVFSAFSVF"
     gene            499..2931
                     /gene="hsdR"
                     /locus_tag="ACHKAY_002880"
     CDS             499..2931
                     /gene="hsdR"
                     /locus_tag="ACHKAY_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO5701539.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2998..4467
                     /locus_tag="ACHKAY_002881"
     CDS             2998..4467
                     /locus_tag="ACHKAY_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO5701540.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4467..6236
                     /locus_tag="ACHKAY_002882"
     CDS             4467..6236
                     /locus_tag="ACHKAY_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO5701541.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TRIKTAQQTQLHLADALTDAAIN"
     gene            6457..6798
                     /gene="symE"
                     /locus_tag="ACHKAY_002883"
     CDS             6457..6798
                     /gene="symE"
                     /locus_tag="ACHKAY_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO5701542.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(6845..8008)
                     /locus_tag="ACHKAY_002884"
     CDS             complement(6845..8008)
                     /locus_tag="ACHKAY_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO5701543.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(8056..8938)
                     /locus_tag="ACHKAY_002885"
                     /pseudo
     CDS             complement(8056..8938)
                     /locus_tag="ACHKAY_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(9035..9199)
                     /gene="yjiS"
                     /locus_tag="ACHKAY_002886"
     CDS             complement(9035..9199)
                     /gene="yjiS"
                     /locus_tag="ACHKAY_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO5701544.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            9376..10788
                     /gene="yjiR"
                     /locus_tag="ACHKAY_002887"
     CDS             9376..10788
                     /gene="yjiR"
                     /locus_tag="ACHKAY_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO5701545.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(11030..11974)
                     /locus_tag="ACHKAY_002888"
     CDS             complement(11030..11974)
                     /locus_tag="ACHKAY_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5701546.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            12441..13673
                     /gene="mdtM"
                     /locus_tag="ACHKAY_002889"
     CDS             12441..13673
                     /gene="mdtM"
                     /locus_tag="ACHKAY_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO5701547.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            13714..14994
                     /gene="yjiN"
                     /locus_tag="ACHKAY_002890"
     CDS             13714..14994
                     /gene="yjiN"
                     /locus_tag="ACHKAY_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO5701548.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            15110..16261
                     /gene="yjiM"
                     /locus_tag="ACHKAY_002891"
     CDS             15110..16261
                     /gene="yjiM"
                     /locus_tag="ACHKAY_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO5701549.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGASFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            16271..17038
                     /gene="yjiL"
                     /locus_tag="ACHKAY_002892"
     CDS             16271..17038
                     /gene="yjiL"
                     /locus_tag="ACHKAY_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO5701550.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            17035..17292
                     /gene="ytiA"
                     /locus_tag="ACHKAY_002893"
     CDS             17035..17292
                     /gene="ytiA"
                     /locus_tag="ACHKAY_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO5701551.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            17357..18217
                     /gene="yjiK"
                     /locus_tag="ACHKAY_002894"
                     /pseudo
     CDS             17357..18217
                     /gene="yjiK"
                     /locus_tag="ACHKAY_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            18285..19463
                     /gene="yjiJ"
                     /locus_tag="ACHKAY_002895"
     CDS             18285..19463
                     /gene="yjiJ"
                     /locus_tag="ACHKAY_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5701552.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(19476..20030)
                     /gene="kptA"
                     /locus_tag="ACHKAY_002896"
     CDS             complement(19476..20030)
                     /gene="kptA"
                     /locus_tag="ACHKAY_002896"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO5701553.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            20280..20963
                     /gene="yjiH"
                     /locus_tag="ACHKAY_002897"
     CDS             20280..20963
                     /gene="yjiH"
                     /locus_tag="ACHKAY_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO5701554.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            20960..21421
                     /gene="yjiG"
                     /locus_tag="ACHKAY_002898"
     CDS             20960..21421
                     /gene="yjiG"
                     /locus_tag="ACHKAY_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO5701555.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            21434..22606
                     /gene="iadA"
                     /locus_tag="ACHKAY_002899"
     CDS             21434..22606
                     /gene="iadA"
                     /locus_tag="ACHKAY_002899"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO5701556.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            22671..23583
                     /gene="hypT"
                     /locus_tag="ACHKAY_002900"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             22671..23583
                     /gene="hypT"
                     /locus_tag="ACHKAY_002900"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(23576..23968)
                     /gene="iraD"
                     /locus_tag="ACHKAY_002901"
     CDS             complement(23576..23968)
                     /gene="iraD"
                     /locus_tag="ACHKAY_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO5701557.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(23965..24048)
                     /gene="idlP"
                     /locus_tag="ACHKAY_002902"
     CDS             complement(23965..24048)
                     /gene="idlP"
                     /locus_tag="ACHKAY_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO5701558.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(24164..24307)
                     /gene="ytiD"
                     /locus_tag="ACHKAY_002903"
     CDS             complement(24164..24307)
                     /gene="ytiD"
                     /locus_tag="ACHKAY_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO5701559.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         6181 a         6021 c         5936 g         6202 t
ORIGIN      
        1 cgaatactga aaaagcacta aaaacgtata gagaggcact gactgaaaaa atattaattg
       61 acaacactaa aaacaaaaat ataaaacacg caataagtgc acaccaggca gcacaactat
      121 tcaacctgtt tttattactg aacttaacaa aataccccat atattctgta atgcgatttt
      181 cttccactaa tgcataaaga agaataccaa caaagcacag agataaaata atatctttcc
      241 acagatattt ataagggaga agtataaaac caagaaatag ataaattagc agaatgacat
      301 ctaatgcctt agtacaaaaa tattgtttca ctccttgcaa gaaccgaaca ttctccatat
      361 gaatccttat cctactattg atgaaactca agaatacctt taaaacagtg acttaacgct
      421 gataatactc tttgaacaga tgaattcatc tatttttctg tgtagaattt gcgcatcgca
      481 tttagatgag aacaccccat ggcggaactg aacctaagta acctgacgga agcagacatc
      541 attaccaaat gcgttatgcc agccattctc aatgcaggct gggacaacac aacgcaaatc
      601 agacaggagg tcaaactccg ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc
      661 agaacggtaa aatctgctga catcgtgctg tatcacaaac caggcattcc cttagccgtg
      721 attgaagcaa aagccaacaa acatgaaatt ggcaaaggga tgcaacaggg cattgaatat
      781 gcgcgcctgc tggacgttcc ttttgttttt gccaccaacg gggatggctt tatcttccgc
      841 gatgccaccg cagccgaagg tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc
      901 tcccccgctg aactctggca aaaattctgc ctctggaaag gatacacaca agctcagctt
      961 ccggtgatta ctcaggacta ttacgacgat ggcagcggta aatcgccacg ttattaccag
     1021 cttcaggcaa tcaacaaaac cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg
     1081 gtcatggcga ccggaacagg gaaaacctat accgcattcc agatcatctg gcgcctgtgg
     1141 aaatcaaaaa ataaaaaacg cattttgttc cttgccgatc gcaacattct ggtcgaccaa
     1201 accaaaaata atgatttcca gccatttggt acggcaatga ccaaagtcag cggacgcacc
     1261 attgatcccg cttatgaaat tcacctcgcg ctctatcagg ctataactgg cccggaggaa
     1321 gaccaaaaag cgtttaaaca agtcgcacca gatttcttcg atctgatcgt gatcgacgaa
     1381 tgccatcgcg gcagcgcatc cgaagacagc gcctggcgag agatccttga ttacttcagt
     1441 tccgccaccc aaattggctt aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg
     1501 gattacttcg gcgatccggt ttacgtctac tcgctaaaag aagggatcga agacggcttc
     1561 ctcgcccctt ataaagttgt ccgtgttgat attgatgttg atctgcaagg ctggcgccca
     1621 accaaagggc aaactgactt aaacggcgaa gtgatcgacg atcgtatcta taaccagaaa
     1681 gatttcgatc gcacgatggt aatcgacgaa cggacagaac tggttgccag aaccattacc
     1741 gactacctca agcgcaccaa cccgatggat aaaaccatcg tcttctgtaa cgacatcgat
     1801 catgcagaac gtatgcgccg cgccctggtg aatctcaacc cggagcaggt gaaaaagaac
     1861 gacaaatacg tcatgaaaat caccggcgat gatgaaattg gcaaagctca gttggataac
     1921 ttcatcaacc cgaaaaaagc gtacccggtt atcgcgacca cttcagagct gatgaccact
     1981 ggtgtggatg ctaaaacctg caaactggta gtactggacc agaacatcca gtcgatgacc
     2041 aaattcaagc aaattatcgg tcgtggtaca cgcatcgacg aacgttacgg caaactctgg
     2101 tttaccatcc tcgactttaa aaaagccacc gaactgtttg ccgatgagcg tttcgatggc
     2161 attcccgaaa aagtcatgga taccacacca gaggatatcg ccgatccaga atctgatttt
     2221 gaagagaaac tcgaagaaat cagcgaacat gacgacgaac aggtaacagg cgttgatgaa
     2281 ccgcctgcgc caccatacca ggttaccgat accgatgatg tcggcccact tccggaagaa
     2341 gacgagaaga aaatccgcaa gtttcacgtc aacggtgtag cggtgggcgt tattgcgcag
     2401 cgtgttcagt attacgatgc cgacggtaaa ctggttaccg aatcttttaa agattacacc
     2461 cgcaaaacac tgctcaaaga atatgcctcg ctggatgact ttacccgcaa gtggcaggac
     2521 gccgatcgca aagaagcgat cattcacgag ctggagcaac aggggatcat ctgggaagta
     2581 ctggcagaag aagtcggtaa agatctcgac ccgttcgaca tgctttgcca cgtagtgtat
     2641 ggtcagccgc cgttaacccg caaagagcgc gccgagaacg tgcgcaagcg gaactacttc
     2701 acaaaatact ctgaagcagc gcaagccgtg ctcgataatc tgctggataa atacgccgat
     2761 gcgggcgtac aggagatcga aagtattcag gtgctgaaac ttaaaccatt cgacagcatg
     2821 ggcactttac cggagattat taaaaccgga tttggcgacc gtaacgggta taatcaggcg
     2881 ctcagcgagc tggaaaacga aatctaccaa ttaccgcccc gctctgctta acaactctct
     2941 tttgtgcctt ctgcaaccaa catgttgcag aaggccttgt ttttcatgga atacaacatg
     3001 tctatcagtt cagtaatcaa atcattacaa gatattatgc gcaaagatgc cggtgtggat
     3061 ggcgatgcgc agcgtctcgg tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc
     3121 caggaagagg tgctggaact ggagcaggat aactatcaat atccgatccc acagcgttat
     3181 ttatggcgca gttgggccgc aaacgctcag ggcattaccg gtgattccct gctggaattc
     3241 gttaatgatg atctgttccc ggcgttgaaa aacctcactg cgcctatcga taaaaaccca
     3301 cgcggctacg tggtgaagca ggcgttcagc gatgcctata actatatgaa aaacggtacg
     3361 ctactgcgcc aggtgatcaa caagctgaac gaaattgact ttaccagcgc cagcgaacgc
     3421 catctgtttg gtgatattta cgaacagatc cttaaagatc tgcaatctgc gggcaatgct
     3481 ggcgaattct atacgccacg cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa
     3541 ctcggcgaat ccattatgga cccggcctgc ggtacgggcg gttttcttgc ctgcgcattt
     3601 gatcatgtaa agaacaaata cgtgaagagc gtcgccgatc atcagacgct gcaacaacag
     3661 atccacggtg ttgagaaaaa acagcttccg cacctgctgg cgaccaccaa tatgctgctg
     3721 cacggcattg aagtaccggt acaaattcgt cacgacaaca ccctgaacaa accgctttcc
     3781 tcctgggatg aacaactgga tgtcattgtt accaacccgc cgtttggtgg cacggaagaa
     3841 gacggtattg agaagaactt tccggcagag atgaaaaccc gcgaaacggc ggatctgttc
     3901 ctgcaactga ttgtggaagt actggcgaaa aacggtcgcg cggcggtggt attgccggat
     3961 ggcacactat ttggcgaagg cgttaaaacc aaaatcaaaa aactgcttac cgaagagtgc
     4021 aacctgcata ccatcgtgcg tttaccgaat ggggtgttta acccctatac cggcattaaa
     4081 accaacctgt tgttctttac caaaggtcag ccaaccaaag agatttggtt ctatgagcat
     4141 ccttatccgg cgggcgtaaa aaactacagc aaaaccaagc cgatgaagtt tgaagagttt
     4201 caggcggaga tcgactggtg gggtaacgag gccgatggtt ttgccagccg catcgagaat
     4261 gagcaagcgt ggaaagtcag cattgatgac gtgattgcgc gtaacttcaa tctggatatt
     4321 aaaaacccac atcaggcgga aaccattagc catgatccgg acgaactgtt agcgcagtat
     4381 gcaaaacagc aggcggagat ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct
     4441 gcgctgtctg gcaaggaggt taactaatga gtgtggaaaa gctgatcgtt gatcatatgg
     4501 aaacctggac ctcggcgttg caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga
     4561 ttgatttgta tggtattaag aaattgcgtg agctgattct ggaactggcg gtgcgaggta
     4621 aactggtgcc gcaggatccg aacgatgaac cggcgtcgga gctgctgaag cgtattgcgg
     4681 cggaaaaagc agagctggtg aagcaaggta aaattaaaaa gcaaaaaccg ctgccggaaa
     4741 ttagcgagga agagaagccg tttgaattgc cggaggggtg ggaatggact cgattaatta
     4801 atcttggtat atgggcttta gggtctggtt ttccaaatgt ggtacaggga agcactgata
     4861 aagaaatttt gatgtgcaaa gttagcgaca tgaatcttga aggtaacgag aaattcattt
     4921 tttcgacaaa aaatacaatt tcaaaagacc tagctgatga atataaaatt aaaatctcag
     4981 aacccggcac catcattttc cctaaaattg gcggagcaat tgcaactaat aaacgaagaa
     5041 ttttagttca ggatacagct attgataaca actgtcttgg aataaaacca tgtgatgcaa
     5101 tatcggggga gtggttctat ctaatcctta atactctcga tatgtcgaag tatcaatcag
     5161 gaacctcaat acccgcgata aatcaaagtg tcataggtag tataccaatt gcgcttcctt
     5221 ctttaaaaat gcaagagaag attgtttctt atgtcataac cctaatgtcc ctttgcgacc
     5281 aactggaaca gcaatccctg accagtctgg acgcacatca gcaactggtt gaaaccctgt
     5341 tgggaacact tacagacagc caaaacgccg aggaactggc tgaaaactgg gcgcgtatta
     5401 gcgagcattt cgacacccta tttaccaccg aagccagcgt ggatgcgtta aaacagacca
     5461 ttctgcaact ggccgtaatg ggcaaacttg tgccgcagga tccgaatgac gaaccagcct
     5521 ctgaactgct caaacgaatt gcgcaggaaa aagctcaact ggtgaaagaa ggaaaaataa
     5581 aaaaacaaaa accgttgccg ccaattagcg atgaggaaaa accgtttgaa cttccggaag
     5641 ggtgggagtg gtgtaaattt ggtttaacat cagagtttat aaatggcgat cgtggaagta
     5701 attaccccaa taaaaatgag tatgtttctc aaggtattcc ttggatcaat actggacata
     5761 tagaaaaaaa tggtacactt acggttacag aaatgaattt tataaccgaa ggaaaattta
     5821 atgaattaag aagtgggaaa attcaaaaag gtgatttggt ttattgccta cgaggagcaa
     5881 cttttggtaa aactgccttt gtaatcccct atgaaacagg agctattgcc tcatcactaa
     5941 tgatcattag accatttatc acagaaatgg gagggtatat ttataattat ttaaccagtc
     6001 catttgggcg ctcacagatt taccgctttg ataatggctc agcacaaccc aacctatcag
     6061 caaatagtgt aatgttgtat tcattcccat gcccaccatt aactgaacaa tatagaatat
     6121 tttcacaagt aggtttactt catgaactat gcgataagct taaaactcgt ataaaaacag
     6181 cccaacaaac ccaactgcac cttgcagacg cactcactga cgcggcgata aactaatttt
     6241 ctcgccctca aaaaaccaat aaagcggcta aatggccgct ttattcacat caccaaaaat
     6301 tatatttcac gctaattttc atcttatttt cattaatttt tagctggact ttccctgtat
     6361 ttactgatga tatatccagg tatttagcgc ggtgcggatg tgcgccaaca cacccgcacc
     6421 gctaatcaca atcactatta tcggagaata gcagttatga ctgacacgca ttctattgca
     6481 caaccgttcg aagcagaagt ctccccggca aataaccgtc aattaaccgt gagttatgcg
     6541 agtcgctacc cggattacag ccgtattccc gccatcaccc tgaaaggtca gtggctggaa
     6601 gccgccggtt ttgccaccgg cacggcggta gatgtcaaag taatggaagg ctgtattgtc
     6661 ctcaccgccc aaccacccac cgccgaagag agcgaactga tgcagtcgct gcgccaggtg
     6721 tgcaagctgt cggcgcgtaa acaaaagcag gtgcaggatt ttattggggt aattaccggt
     6781 aaacagaaag tcgcctgata aattgacccg gtagcgccag tgctaccggg tattattcgc
     6841 cgcgttatac caacgcgctc tccatctgtt tttcaaatgc ctgacgaatt aatcccataa
     6901 tgtaaggaag ttgtgcgagg tcgctgaaac caatttctac atcgccatta ccccagcgcc
     6961 caacatttgt cacatctttg gcaatacctt tcggatcgac cagttcatga aactgcatat
     7021 taagcgacaa ccgcagtcgg cttttttgcg gtacaacatc aacaaaattc gtttcagctt
     7081 taaacgcaat atagagcttc aatacttcct gcgtgatccc tgcgtccagg cgcataactt
     7141 catcgcgaag atgatcgaat aacacccggc tatgcgaacc atcagcaaga aaaggataat
     7201 cactcaggct gtaactggtt ttgttttcag gctgcccccg ataaacagct aaaacctctt
     7261 cagaaagaga aggcgcttgc cagacctgaa ccgcgagatc ggccagacga tcggcacggg
     7321 cgcgaatggc ggcttcatcc catttttcgc actgcccaag accgatattc aaatagagcg
     7381 ggctatgttt aaagccgtct tcaatatcgc gtttttccgc gaaaggtctg tcactgtagc
     7441 gggagttata gcgcgttaaa gtgagattcc ccaacgtatg taacagttct ttatgcacac
     7501 gctgccagtc gcttcccagc tcttcacgcc atttagccga cagattttcg ttctgcggca
     7561 taatgtgctc aatcgtaaac tcttccacgc gctctctgcg tttatcgttt tccagtcttc
     7621 gtaaccagta gctgcggctg cggaagttat agagatcgcg aacttttaat tcccgtttaa
     7681 attcatcatc gttggggaaa cgacgatatg aaggcaggtt cataaaatgt acctgaatac
     7741 tttccagata tttttcttta ttaatgacct tataaaaagt ggcaaacgtc ttgttcaaag
     7801 aattcgtcgg aattgcacat actgcacggc ggaaaacata agattcaatt aaacgaatta
     7861 tgctcaggaa atcttcgtga gacaaatcgt catttttata gtcatgataa agcgccagta
     7921 agaaaggata cgccacatca acctttaact cgcgcaaatc ctgaaaagcc gtagcaagcg
     7981 atttgtcact ttcttttccc aatgccattg cacagtaata ctccgcgtag atatgaatat
     8041 cttcaaccag ccgatctacg cctttttcag caacactctg gctgcgggca tggagtttaa
     8101 atgcctcata gacttcatct gtccgaggga tctcccccgt ttttaccgtc agataatgac
     8161 gcataaaact gtcaaaatat tcgctgtaac cttgctgacc aaaggcgact tccattggac
     8221 gccagtgatc ttcatacaac cgggtttgat gctctggttc gaggcccatc agaataaaat
     8281 tgcgcaccag atcggcctga cttaacgcct taccggtgga gttcatactt tcaaaaatca
     8341 gttgcggatt atcctgacca cgattaagcg ccacatcgac aattaataac tttgctaacc
     8401 cacgacataa ggggatcaag tcatcaccga gtttggcaat ttgttcatca aagaaagtaa
     8461 agttttccat tattcgatgg gaatagtttt ctggcattgg tctttgtttt attaacgcca
     8521 gtaaactgtc tttatcagtc tcggtcagta gtaatttaaa acctttctcg ccggactcat
     8581 aaggattgag caaataataa ttacgcaatt tcattgctga aaagccatca aaaacttcgt
     8641 cttcgccaag atggcgcgat aaagcctcaa tcagcaacat tgcggtcgtc agccgttgtt
     8701 gaccatcaat gaccagtaac ggagaaatac cagaaacctg atacaacccc tgctcaatat
     8761 aaacaaccga accgataaaa tgcgctgata tatcgtcacg ctttccggca cgaatgatgt
     8821 cgtcccaaag ttgccgacat tgttgttctg tccatgaata agtccgttga taaatgggaa
     8881 taacaaactg ctgcgaacgt tttaaaaaat cgagtaggcg ggcttctgta gctttcattc
     8941 tgacgtcttt ccgtgaagcg atgatttcgc aataataacc acttcctttt ggaggttata
     9001 gagaaatcct gtttatccgc gatgaattgc cccctcactc cacatcctcc ctgcgtaacc
     9061 caatatctct caaccgctca tcactcatct gctgtaacac tcgtcgggtc tgcatttgcc
     9121 gccgccaacg cctcaccgcc tgccagagtt gcacaagtct gataaacggc gttttggctt
     9181 tgttttcgtg aaattccatc tgctgcctcc tcatctttca gaggcttcat catccacctt
     9241 ctggcagttc acaatacaga tgcaaatatt agttttattt aacatacaga caggttaaaa
     9301 cagcatctgc atccggtttt ttagcctcat ctgtaccggt ttttctttct gtatggtcca
     9361 aataaggaat acagcatgac gcgttatcaa catctggcga ctctacttgc cgagcggatt
     9421 gagcaagggc tgtatcgtca tggggagaaa ttgccgtcgg tgcgtagctt aagtcgggag
     9481 cacggcgtca gcatcagcac cgtgcagcag gcgtatcaga cgctggagac gatgaagctc
     9541 ataaccccac agccgcgttc gggttatttt gtcgcacaac gtaaagccca gccgccagtg
     9601 ccgccgatga cgcgtccggt gcagcgcccg gtggaaatta cccagtggga tcaggtgctg
     9661 gatatgctgg tggcgcatag cgacagttcc attgttccgt taagcaaaag cacgccggat
     9721 gtcgaaacgc ccagcctgaa accgctctgg cgggagctaa gccgggtggt gcagcataat
     9781 ctgcaaaccg tgctcggtta tgacttgtta gccggtcagc gggtattgag agagcagatt
     9841 gcccgcctga tgctcgacag cggctcggtg gtcaccgccg atgacatcat tatcaccagc
     9901 ggctgccata actcgatgtc gctggcgtta atggcggtgt gtaaaccggg cgatattgtc
     9961 gcggtcgaat ctccctgtta ttacggttcg atgcagatgc tgcgcggcat gggcgtgaaa
    10021 gtgattgaaa tcccaaccga tccagaaaca ggcatcagcg ttgaagcgct ggaactggcg
    10081 ctggaacagt ggccgattaa aggcatcatt ctggtgccaa actgtaataa tccgctggga
    10141 tttattatgc cggacgcacg caaacgggcc gttctctctc tcgctcagcg tcatgatatt
    10201 gtgatttttg aagatgatgt ctacggcgaa ctggcaacgg agtatccgcg cccgcggacc
    10261 atccattcat gggatatcga cgggcgagtg ctgttgtgca gctcgttcag taaaagtatt
    10321 gctccaggcc tgcgcgtggg ttggggcgca ccggggcgtt atcacgataa actgatgcat
    10381 atgaaatacg ccatcagcag ctttaatgtg ccgtccacgc aaatggcggc ggcaacgttt
    10441 gtgctggaag gtcactatca tcgccatatc cggcggatgc ggcagaacta tcagcgcaat
    10501 ttggcgcttt atacctgctg gatacgggaa tattttccct gcgaaatctg tattacgcgc
    10561 ccgaaaggcg gatttttact gtggatagaa ttgcctgaac aggtcgatat ggtctgcgtt
    10621 gcgcggcagc tgtgccgcat gaatatccag gtggcagcag gctcgatttt ctcggcttcc
    10681 ggcaaatacc gtaattgtct acgcatcaac tgcgctttgc cgctcagcga aacctatcgc
    10741 gaagcactaa agcaaattgg cgaggccgtg tatcgggcaa tggaataaat tcgaattagt
    10801 ggcgaagcac ctgatttcct ctcacgaatc gcctgtaact cgttgtatct ttgcagggtc
    10861 atcgccacgt tccagcatgg cttaggcaaa gtgtgcttcg ttcataccgg atgcggcgtg
    10921 aacgccttat ccggcctaca aaccgcgcaa attcaataga ttgcagagga aatgtaggtc
    10981 tgataagcgt agcgcatcag gcagctttgc gtttgtcatc cgtctctgat tagtattcga
    11041 tccatggatc gcgcttgatt tcaggcaagg gctgctctaa tgcctgcatt tgctctgtcg
    11101 aaagtccggt aatcttttgt atccattcag gatctgcccc attctgcaac aacaatctaa
    11161 ggaggcgttg ttccccttct tctttcccct tttccattcc caacagccat ccatcattat
    11221 aaattcgctc tgcaatcgtc attattcgct ccctgtgttg tggcattcga cgggtaagtt
    11281 cgctgataaa cttcttaaaa cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg
    11341 cagttatctg gctgtcatta gcacactctg taaccagtaa tactaccagt tggtcgataa
    11401 gccccatcag atcgcgctgg cgaatatgct tttggatcaa ctccaacagg gcgactctgc
    11461 gatgctgcac aatctcgtcg tctggcacga cagtgatatc caccagcggg aacgcggcgg
    11521 tataaagttt ccgtgcggta gttgggtcgg caaattcgtc cagccagcac agggaccagg
    11581 gataaggact acggctaccg tgataaaata gcatcggtat aaccagcggt agcgcccgac
    11641 gtttatcatg ctcgatatgg cgctgcatca ccgccatgga atagcgcatc aggcgaaagg
    11701 ccatatgaat atcctcacgg ctctgatgtt caatcaccac ataaatatag ccatcgcctt
    11761 cgcgggtatt taccgaccac agaatatcgg agtgtagcgc ccgcaatttt tcatcgacaa
    11821 agctggcgga ttccagtttt aagctgtcga gatcgcacag ttcacgtaaa tctttgggta
    11881 aatagatctc cataaaatcc cgcgcggtgt cagggtgggt gagaaaggat ttaaataatg
    11941 cgtcatgcgg cgtgctggtt gtgaagtttg tcatggcgat ccgtcacctg ttgataagat
    12001 ggcccgactt tagcgactaa caaaaccatt cacacctggc aatttcccgt tccccgaggc
    12061 gctttccagg ctatttttgt tcctctgcaa gcgatacatt tttccggaaa caggctcgcg
    12121 ttttacattt cctgtacccg gtagatattc ccctttccac cgcgctcttt tgattaatcg
    12181 caaattttaa ttacacttaa ggtgtatatt ttctatgcaa accatcaatt caagaggtgt
    12241 aatgtgctga tgactatttg taatcgttat acattctgac ccgaactcag aaggtatttc
    12301 tctgtctgta taagcacagg cagtgtggtt gattacatga aattcagtac atttgtcctt
    12361 ctcgcctacc tttcgtcatt accgacggta ttgaatttcg ttttccccgt tggggttctc
    12421 cggacaagga gttgtttgtt atgccacgtt tttttgcccg ccatgccgcc acgctgtttt
    12481 tcccgatggc gttgattttg tatgattttg ccgcgtacct gtcgacggac ctgattcagc
    12541 cggggatcat taatgtggtc cgtgatttta atgccgatgt cagtctggcc ccggcagccg
    12601 tcagtctcta tctcgcgggc ggtatggcgt tacagtggct gctggggccg ctttccgaca
    12661 ggattggccg caggccggtg ctgattaccg gggcgctaat atttaccctt gcctgcgctg
    12721 cgacaatgtt cacaacgtct atgacacagt ttcttatcgc gcgtgcaatt cagggcacca
    12781 gtatctgttt tattgctacc gttggttatg tcacggtgca ggaggcgttc ggacagacaa
    12841 aagggatcaa gttgatggcg attatcacct ccatcgtact gattgcgccg attattggcc
    12901 cgctttccgg cgcagctctg atgcactttg tgcactggaa agtccttttt gccatcattg
    12961 cggttatggg ttttatctca tttgttggct tactgctggc gatgccagag acggtgaagc
    13021 gcggcgcggt tccatttagc gccaaaagcg tcttgcgcga ttttcgtaat gtcttttgca
    13081 atcggctgtt cctctttggc gcagcaacca tctctttaag ctatatccca atgatgagtt
    13141 gggtggctgt ctcgccggtg atcctgattg acgcaggcgg cttaacaact tcgcagttcg
    13201 cctggacaca ggttccggtg ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt
    13261 tcgttaaaga tccgaccgaa ccgcgcttta tctggcgtgc cgtacccatt caactggtcg
    13321 gcctcgcact gttgattatc ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc
    13381 tgggcaccag tctgtatgct ttcgggattg gtttaatctt cccgacctta ttccgcttta
    13441 cacttttttc caataactta ccgaaaggga ccgtctccgc atcgctgaat atggtgatcc
    13501 tgatggtgat gtctgtctcg gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct
    13561 tgccgtttca tctgttagcc gtcgtagcgg gagttatcgt cgttttcacc ctggcggggt
    13621 tgctcaatcg cgtgcaccaa catcaggctg ccgagctggc ggaggagcgg tgatttttgc
    13681 gcgatcctgc cgtcaggctc tattcttaac gttatgaata aactcattga actcagacgc
    13741 gccaaaatgt tggcgctctc tttactgctt atcgccgctg ctacctttgt cgttacgctg
    13801 tttttgccac ccaatttttg ggtgagcggc gtgaaggcaa ttgctgaagc ggcgatggtc
    13861 ggcgcgctgg cggactggtt tgcggtggtg gcgctgtttc gccgcgtgcc aattccgatc
    13921 atttctcgtc atacggcgat tatcccgcgt aataaagacc ggattggcga aaatctcggt
    13981 cagttcgtgc aggaaaaatt tctcgatacc cagtcgctgg tggcattgat tcgacgtcac
    14041 gaaccggcgt tgctaattgg taactggttt agccagccag aaaacgcccg ccgcgttggt
    14101 cagcatctgt tgcagatcat gagtggtttt cttgaactga ccgatgatgc acgtattcag
    14161 cgcctgctta agcgcgcggt ccatcgggcg attgataagg tcgatctttc cggaaccagt
    14221 gcgttgatgc tggagagtat gaccaaaaac gatcgtcatc aggtgctgct cgatacgctg
    14281 atcgcacagt tgattgctct tctccagcgc gataaatcgc gcaagtttat cgcccaacaa
    14341 attgttcgct ggctggagag tgagcatcca ctgaaagcca aaattctccc caccgaatgg
    14401 ctgggcgaac atagcgcgga gttggtttct gacgcggtga attctttgct tgatgatatc
    14461 agccgcgatc gtgcgcatca gatccgccat gcatttgatc gcgccacttt tgccctgatc
    14521 gacaagttga aaaacgatcc ggaaatggca gcgcgagctg atgccgtaaa aagctatctg
    14581 aaagaggatg aagcctttaa ccgctatctc agtgaattgt ggggggattt acgggaatgg
    14641 ctgaaagcgg atatcaacag tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt
    14701 caatggtttg gcgaaacgtt aattgccgat gatgccttgc gggcgtcgtt aaatggtcat
    14761 ctggaacaag ccgcgcaccg cgtcgcacct gagttttccg cattcctgac gcgccacatc
    14821 agcgatacgg taaaaagctg ggatgcccgg gatatgtcgc ggcaaataga gttaaatatc
    14881 ggcaaagatc tgcagtttat ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta
    14941 attttatatt tgctgtcaca gctcccggcc ttgttccccc tcggcaattt ttagaaatcc
    15001 atgaaaaact aagtggctaa ccgcgagaga gatcaaataa accacactaa aagaatggta
    15061 ataatgtggc ctcttttcat tatctcccgt ggtacgggga aggaaaatca tgtcacttgt
    15121 caccgatcta cccgccattt tcgatcagtt ctctgaagct cgccagaaag gctttctcac
    15181 cgtcatggat ctcaaggagc gcggcattcc gctggttggc acttactgca cttttatgcc
    15241 gcaagagatc ccgatggcag ccggtgcggt tgtggtttcg ctctgttcca cctctgatga
    15301 aaccattgaa gaagcggaga aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag
    15361 ctacggcttc ggtaaaaccg ataaatgccc ctacttctac ttttctgatc tggtggtcgg
    15421 tgaaaccacc tgcgacggca aaaagaaaat gtatgaatac atggcggagt ttaagcctgt
    15481 tcatgtgatg cagctgccaa atagcgttaa agacgatgcc tcgcgtgcgt tatggaaagc
    15541 cgagatgctg cgcttacaaa aagcggtgga agaacgtttt gggcacgaaa ttagcgaaga
    15601 tgctctgcgc gatgccattg ctctgaaaaa ccgcgaacgt cgcgcactgg ctaattttta
    15661 tcatcttggg cagttaaatc ctccggcgct tagcggcagc gacattctga aagtggttta
    15721 cggcgcaagc ttccggttcg ataaagaggc gttgatcaat gaactggacg cgatgaccgc
    15781 ccgcgttcgt cagcagtggg aagaaggcca gcgactggac ccgcgtccac gcattttaat
    15841 caccggctgc ccgattggcg gcgcagcaga aaaagtggtg cgcgcgattg aagagaatgg
    15901 cggctgggtt gtcggttatg aaaactgcac cggggcgaaa gcgaccgagc aatgcgtggc
    15961 agaaacgggc gatgtctacg acgcgctggc ggataaatat ctggcgattg gctgctcctg
    16021 tgtttcgccg aacgatcagc gcctgcaaat gctcagccag atggtggaag aatatcaggt
    16081 cgatggcgta gttgatgtga ttttgcaggc gtgccatacc tacgcggtgg aatcgctggc
    16141 gattaaacgt catgtgcgtc agcagcacaa cattccttat atcgctattg aaacagacta
    16201 ctccacctca gatgtcgggc agctcagtac ccgtgtcgcg gcctttattg agatgctgta
    16261 aggagtggca gtgacatatt cgattggcat tgattctggt tctaccgcca ccaaagggat
    16321 cttactggca gacggcgtga ttacgcgccg tttcctcgtt ccaacaccct ttcgtccggc
    16381 aacagcaatt actgaagcct gggaaactct gcgcgaaggg ttagagacaa cgccgtttct
    16441 gacgctcacc ggctatggac ggcaactggt ggattttgcc gataaacaag tgacggaaat
    16501 ctcctgtcat gggctgggcg cacggtttct tgcgccagca acgcgcgcgg taatcgacat
    16561 cggtggtcag gacagcaaag tgattcagct tgatgatgac ggtaacctgt gcgatttcct
    16621 gatgaatgac aaatgcgcgg cgggcaccgg gcgtttcctg gaggtgatct cgcgcacgct
    16681 tggcaccagc gtcgagcaac tcgacagcat taccgaaaat gtcacgccgc acgccatcac
    16741 gagtatgtgc acagtgtttg ctgaatcaga agcgatcagc ctgcgctcag cgggcgtcgc
    16801 gccagaagcg attctcgccg gagtgattaa cgcgatggcg cggaggagtg ccaatttcat
    16861 tgctcgtctc tcctgtgaag cgccgattct gtttactggt ggcgttagtc attgccagac
    16921 gtttgcccgg atgctggaat ctcatctggg aatgccggta aatacccatc ctgatgcgca
    16981 atttgctggc gcaattggcg cggcggtaat tggtcaacga gtgaggacac gccgatgaaa
    17041 gagtttttat ttttatttca ctccacagtc ggcgtcatac aaacccgcaa agcgttgcag
    17101 gcagcgggca tgacttttcg cgtcagcgat attccccgtg atttacgcgg cggctgcggg
    17161 ttatgcattt ggctgacctg tccccccggc gaggaaatgc aatgggtgat ccctgggctg
    17221 actgagtcaa tttattgtca gcaggatggt gtctggcgct gcattgcaca ttaccgggtt
    17281 tctccgcgtt aaggattcat taaggtcgct acgtatactt ctcgatatct tattgatttg
    17341 ccttaaaggg attaccgtga caaaaagtat ttcgttaagt aaacgcatct ccgttattgt
    17401 gattcttttt gccattgtgg cagtgtgtac tttcttcgtg caatcttgcg cccgtaaaag
    17461 caatcatgca gcataatttc agaattacca cgccaccatt gacggcaaag aaattgcagg
    17521 cataacgaat aatatctctt cgctgacctg gtcggcacaa agcaatactc tttttagcac
    17581 tatcaacaaa cctgccgcca ttgttgaaat gaccaccaat ggtgatttta tccgcacaat
    17641 tcctctggat tttgttaagg atctcgagac gatcgaatac attggcgata atcagtttgt
    17701 cattagcgat gagcgcgatt acgccattta tgtgatttca ctgaccccaa actcggaagt
    17761 aaaaatcctc aaaaaaataa aaatcccgct gcaagactcc ccaaccaatt gcggttttga
    17821 agggttggct tattctcgcc aggatcacac tttctggttt tttaaagaga aaaatccgat
    17881 tgaggtctac aaagttaacg ggctgttaag tagcaatgaa ttacacatca gcaaagacga
    17941 ggcactacaa cggcaattta ctttagatga tgtgtccggc gcagaattta accagcaaaa
    18001 aaatacgctt ctggtgctgt ctcatgaatc tcgcgcactc caggaagtaa ccctggttgg
    18061 cgaggttatc ggtgaaatat cactaaccaa aggtagtcga ggtttgtcgc ataacatcaa
    18121 acaggccgaa gggattgcga tggatgcctc tggtaatatt tatatcgtta gcgaaccaaa
    18181 tcgtttttat cgctttaccc ctaaatcatc gcattaacag gctggcatat ttcacctttt
    18241 tgttattaac ttgttaacaa caaaaatgac atacaaggtt aaacatgcct tcgtcctcgc
    18301 atcccgtaga acgcttttct ttcagcaccg cgcttttcgg gatgctggtt ctgaccttag
    18361 gtatgggatt aggtcgcttc ctttatacgc ctatgttgcc cgtcatgatg gcggaagggg
    18421 cgttttcatt tagccagctc tcgtggattg ccagcggcaa ctatgcgggg tatctggctg
    18481 gcagtctgct attttcgttt ggcgcatttc accagccgtc gcgcctgcgc ccatttctgt
    18541 tggtttctgc cctggcgagc gggttgttga tcctcgcaat ggcatggttg ccgccattta
    18601 ttctggtgtt attgattcgc ttcctggcgg gtgtcgccag cgccggtatg ctgatttttg
    18661 gttcgacgct gattatgcag cacacgcgcc atccttttgt gctggcagct ttgttttctg
    18721 gcgttggcat tggcattgca ctgggcaacg aatatgttct ggcaggcctg cattttgcct
    18781 tatcctcgca aacgttatgg caaggcgccg gggcgctttc tggcatgatg ctgattgcac
    18841 ttacgctttt aatgccctcg aaaaaacacg ccatcgcacc aatgccattg gcaaaaacgg
    18901 agcaacagat aatgagctgg tggttactgg ctattctgta cggcctggcg ggatttggtt
    18961 atatcatcgt cgcgacttac ctgccgctca tggcgaaaga tgcaggctca ccattgttaa
    19021 ccgcccatct ctggacgtta gtcggcttat caattgtgcc aggctgcttt ggctggctat
    19081 gggcagcaaa acgttggggg gctttgcctt gcctgacggc gaatttgctg gtgcaggcta
    19141 tcagtgtgct gcttactctc gccagcgact ctcctctctt gcttatcatt agtagtatcg
    19201 gttttggcgg tacctttatg ggcacgacat ctctggtgat gactatcgcc cgccaactga
    19261 gcgttccggg aaatctcaat cttttaggct ttgtgacgct catttacggc attggacaaa
    19321 tcctcggccc ggcgctgacc agtatgctcg gcaatggaac atcggcgctt gccggcgcca
    19381 ctctctgcgg cgcggcggcg ttgtttattg ctgcgcttat cagcacagta caattattta
    19441 agctgcaagt ggtcacttca tgaggagtgt ttcccttacc actcaagaaa ttcgaccgca
    19501 accgttgatg tcagccagac cccgttttcc gtttgccaga aagggatgcc tcgtttcgcc
    19561 atttcctgtg ctttgacggt taagataacc ggcgatccgt gtcttgctcc cactttgcgt
    19621 gccgttgctt catcagcaga gagatgtacg tagtgacgct cacctgcaat cagcccctgt
    19681 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt aaagaaactg tggcggtgtt
    19741 ttttcggcaa aggaaatagc gacctgcgaa gtcaaatgcc cctgaaccgc gcggatgcac
    19801 ctaccatcac tggaataact aaaacgcttt ttatcactgg tggcgaccac ggtatccagc
    19861 aatgtacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt atcgatatcc
    19921 gcccatcctt cacggtcaag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
    19981 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat ctcgttttcg
    20041 tccctggatt ttctgctttt atttactgat gtcattgtca ctgggtttat gaattttctg
    20101 caactcaatc ctgcgcagag ccttttccac acagcgtaat ttttccgcta tgcaaaaaac
    20161 gcatagccag aaacatactt gcattttccc catacccaca tggccgataa ggtttttcag
    20221 ctccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
    20281 tgacacaaca aggggatgct gtagccgggg atctggcgac agaaaaagtg ggcattaaag
    20341 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc gggacagaca
    20401 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
    20461 cgaacggagc caccacctca tttcgcgggg cgggtggcgc gggggctaaa gatggctttc
    20521 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
    20581 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
    20641 tgggcattcc agggatctgc tcgctggcgc tgatcgctaa cctgcaaaac actgatgccg
    20701 ccgccggtat gaccaaagag ctggcgcagg aaggggaaat taccgaacgc gataaagtga
    20761 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
    20821 tcgcggtgtt cgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
    20881 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
    20941 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
    21001 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
    21061 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
    21121 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
    21181 cgcattaatg agtatgggtg gcgcagtcgg tgttgccgcc agtctggcaa ccgctggcgc
    21241 attaaccggt cacgatgtca ccgttttact gcccgcgatg tatttaatgg gcaacccggt
    21301 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
    21361 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
    21421 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
    21481 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
    21541 cgccgttgcc agcaatatcc cttctgacat tgtaccggac tgcacggttg tcgatctcag
    21601 cgggcagatc ctctttcctg gttttattga tcaacacgtc catttgattg gtggtggcgg
    21661 tgaagcaggt cccacgacgc gcacgccaga agtggcgcta agtcgcctga cggaagcggg
    21721 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc cggaatccct
    21781 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
    21841 ttatcatgtc ccttcccgca ccattacagg ttccgtggaa aaagacgtgg cgattatcga
    21901 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
    21961 tcacctggct aatatggcgg cggaatcccg cgttggcggt ttgctcggcg gtaaacctgg
    22021 cgtcaccgtg ttccacatgg gcgacagcaa aaaggcgtta cagcctgtct atgacctgct
    22081 ggaaaactgc gatgtgccga tcagcaagtt gctgccgacc cacgttaacc gcaacgttcc
    22141 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc actatcgata tcaccagcag
    22201 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattcc
    22261 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
    22321 agggaattta acccatatcg gtgttgccgg ttttgaaacg ttgctggaaa ccgtgcaggt
    22381 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
    22441 agccggtttc cttaacctga ccgggaaagg cgaaattctg ccaggcaatg atgctgactt
    22501 gctggtcatg acgccagaac tgcgcattga gcaggtatac gctcgcggca aactgatggt
    22561 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
    22621 cccgaatgga cgtagaatgc ccgcaagggc ggctgacaga gtaaaacgta atggatgact
    22681 gtggtgcgat tttgcataat attgaaacca aatggcttta tgattttctg accctggaaa
    22741 aatgccgcaa tttttcccag gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
    22801 gccgcatccg tgcgctggaa caggcgattg gtgttgaatt gtttaaccgc caggtaacgc
    22861 cgctgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
    22921 agttagagag caatctggca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
    22981 agatagccgc tgcacactct ctttccctcg ggctgttacc gtccattatc agccagatgc
    23041 cgccgctctt tacctgggca attgaagcta ttgatgtcga tgaagcggtc gataaactgc
    23101 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
    23161 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
    23221 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
    23281 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagca
    23341 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
    23401 ttgcctggct gccggaatac gctatccaac aagaaattcg cagcgggcag ctcgttgtgc
    23461 tgaatcggga cgaactggtg atcccgatcc aggatctacg cataccggat gaatactcgc
    23521 atgaatcccg ttgccgaacg cttctggcgt gaactgcgcg agctggagat tgtgcttagc
    23581 tgacattctc cagcatcgca ctacaccaac cgccattatc ccagaccaga tcaaacgtta
    23641 gcggttcacc gtcgtggtac agcattatct caaggctgat cgccagagtg tggcagtacg
    23701 cttctttaaa attaacctgc aacgccgcaa tccgtggctc atgaaataac agtgcagaac
    23761 gcagctgacg gcaataccag ctctggagat gtttgtcgct ctggttatgc acttgccact
    23821 cgggcatgcc tgaaacaagt aatgacggta atgacgaatg aggagaatta aaaagagtga
    23881 ggagatcgct gcataccgat ttacgcagca atgacgtttt attgcctgaa atttcaggta
    23941 aaacagcctg acgtgattgg cgcatcattt tgcgcactcc tgacgtttag caacatatgc
    24001 ggcttgccct gaacaccggg caccactgta ttgatgctca ttttccatag ccattccctt
    24061 ttgttcatta catccatgtc agtctgagac aggcctgaat cctgtcagca aagtataaaa
    24121 ccttacttgc ctatgaatat ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa
    24181 tcatcaaact caaaaaaaaa ccgctagtat attctgaata ttccgaatag ttacgtagat
    24241 gaaaatactt gtcaccaccg ctacttaatt ccggtgtgaa attatttatt tccgcataat
    24301 cagccatgta ttatttgaaa caattagttt gtaaataata
//
LOCUS       DAZFKM010000071        23963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFKM010000071 DAZFKM010000000
VERSION     DAZFKM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(31..1485)
                     /gene="xylB"
                     /locus_tag="ACHKAY_002904"
     CDS             complement(31..1485)
                     /gene="xylB"
                     /locus_tag="ACHKAY_002904"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO5701560.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1557..2879)
                     /gene="xylA"
                     /locus_tag="ACHKAY_002905"
     CDS             complement(1557..2879)
                     /gene="xylA"
                     /locus_tag="ACHKAY_002905"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO5701561.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3245..4237
                     /gene="xylF"
                     /locus_tag="ACHKAY_002906"
     CDS             3245..4237
                     /gene="xylF"
                     /locus_tag="ACHKAY_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5701562.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4315..5856
                     /gene="xylG"
                     /locus_tag="ACHKAY_002907"
     CDS             4315..5856
                     /gene="xylG"
                     /locus_tag="ACHKAY_002907"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO5701563.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5834..7015
                     /gene="xylH"
                     /locus_tag="ACHKAY_002908"
     CDS             5834..7015
                     /gene="xylH"
                     /locus_tag="ACHKAY_002908"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO5701564.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7093..8271
                     /gene="xylR"
                     /locus_tag="ACHKAY_002909"
     CDS             7093..8271
                     /gene="xylR"
                     /locus_tag="ACHKAY_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO5701565.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8379..9203)
                     /gene="bax"
                     /locus_tag="ACHKAY_002910"
     CDS             complement(8379..9203)
                     /gene="bax"
                     /locus_tag="ACHKAY_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO5701566.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9523..11553
                     /gene="malS"
                     /locus_tag="ACHKAY_002911"
     CDS             9523..11553
                     /gene="malS"
                     /locus_tag="ACHKAY_002911"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO5701567.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11731..12984
                     /gene="avtA"
                     /locus_tag="ACHKAY_002912"
     CDS             11731..12984
                     /gene="avtA"
                     /locus_tag="ACHKAY_002912"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO5701568.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13136..13609)
                     /gene="ysaA"
                     /locus_tag="ACHKAY_002913"
     CDS             complement(13136..13609)
                     /gene="ysaA"
                     /locus_tag="ACHKAY_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO5701569.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13711..14559)
                     /gene="yiaJ"
                     /locus_tag="ACHKAY_002914"
     CDS             complement(13711..14559)
                     /gene="yiaJ"
                     /locus_tag="ACHKAY_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO5701570.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            14760..15758
                     /gene="yiaK"
                     /locus_tag="ACHKAY_002915"
     CDS             14760..15758
                     /gene="yiaK"
                     /locus_tag="ACHKAY_002915"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO5701571.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15770..16237
                     /gene="yiaL"
                     /locus_tag="ACHKAY_002916"
     CDS             15770..16237
                     /gene="yiaL"
                     /locus_tag="ACHKAY_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO5701572.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16355..16828
                     /gene="yiaM"
                     /locus_tag="ACHKAY_002917"
     CDS             16355..16828
                     /gene="yiaM"
                     /locus_tag="ACHKAY_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO5701573.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16831..18108
                     /gene="yiaN"
                     /locus_tag="ACHKAY_002918"
     CDS             16831..18108
                     /gene="yiaN"
                     /locus_tag="ACHKAY_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO5701574.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18121..19107
                     /gene="yiaO"
                     /locus_tag="ACHKAY_002919"
     CDS             18121..19107
                     /gene="yiaO"
                     /locus_tag="ACHKAY_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO5701575.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19111..20607
                     /gene="lyxK"
                     /locus_tag="ACHKAY_002920"
     CDS             19111..20607
                     /gene="lyxK"
                     /locus_tag="ACHKAY_002920"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO5701576.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            20604..21266
                     /gene="ulaD"
                     /locus_tag="ACHKAY_002921"
     CDS             20604..21266
                     /gene="ulaD"
                     /locus_tag="ACHKAY_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5701577.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            21259..22119
                     /gene="sgbU"
                     /locus_tag="ACHKAY_002922"
     CDS             21259..22119
                     /gene="sgbU"
                     /locus_tag="ACHKAY_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO5701578.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22113..22808
                     /gene="araD"
                     /locus_tag="ACHKAY_002923"
     CDS             22113..22808
                     /gene="araD"
                     /locus_tag="ACHKAY_002923"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO5701579.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(22840..22941)
                     /locus_tag="ACHKAY_002924"
                     /pseudo
     CDS             complement(22840..22941)
                     /locus_tag="ACHKAY_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            22945..23208
                     /locus_tag="ACHKAY_002925"
                     /pseudo
     CDS             22945..23208
                     /locus_tag="ACHKAY_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23155..23895)
                     /gene="yiaT"
                     /locus_tag="ACHKAY_002926"
     CDS             complement(23155..23895)
                     /gene="yiaT"
                     /locus_tag="ACHKAY_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO5701580.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
BASE COUNT         5901 a         6166 c         6054 g         5842 t
ORIGIN      
        1 tcccccaccc ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata
       61 gaggcgacgg aacgtttcac gtcgtggctg ataagcggca taacgctgcg catctggtag
      121 atgcgactgt tctaacggta gctgcggcaa caactcaatg agcgatttct ctggattcgc
      181 cgcaatctgc gccagccttg ctgcgcccag tgctggcccc acatcccccc ccgtacggta
      241 atcgagctgc tgaccgctga tatccgccag catctgacgc cagtactcac tacgcgcccc
      301 gccaccaatc aacgtaacac tttgcggttt aatgccgcag gcatgcacga catccatgcc
      361 atctgccagc gcataaccca cgccttccag cactgctcgc gccagttcat tggggccatg
      421 ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga ttattgtgtg gtgtacgctc
      481 gccggaaaga taaggcagaa accaaactgg ctcggcactt tcatcagcct gttgagctgc
      541 agcgattaaa gctgggacat tgcacaggcc ggttaatttc gcgacccaat ccagacacga
      601 tgctgcactc agcattacag acattaaatg ccaacgctgc ggtagcgcat ggcaaaagct
      661 gtgtacagcg ctttctggct tgcttaagaa cccttcgctg acagcaaaat agacccccga
      721 cgtccccagc gataacattg cctgattagc atcaaccatt cccacaccaa ctgcaccagc
      781 tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc ataccccacg ctttcgcaac
      841 ttcaggtaac aaagcaccag taatttcgct gccttcgtat aatgcgggca tctggtcacg
      901 agataagtcg caagcctgca gcatgacgtc actccagtca cgctttgcga catccagcca
      961 catggtgcca gctgcgtcag acatatcgct ggcaaactcc cccgtcatac gcagacgcaa
     1021 gtaatctttc ggtaataata ctttgtcgat ttgacggaat atctccggct catgccgctg
     1081 aacccatagc aatttaggcg cagtaaatcc gggcatcatc aggttgccgg taatcactcg
     1141 tgattgcgga actctcgctt ccagcaaagt gcactcttgc gcacagcgcc cgtcgttcca
     1201 caaaatggca gggcgcaata cccgttgttg agcatccagt aaggttgctc catgcatctg
     1261 gccggcaata cccaatgctt taacgtcctg cagagaatgc tgatcgccca gagctttcat
     1321 tgcgcgatca gttgcctgcc accactgttc cgggtcttgt tccgaccaga gtggatgcgg
     1381 gcgcgaaacg gtcagctttt ccgtttgcga agcaaccacc tcaccctgct cgttgagcaa
     1441 aataactttt acgcccgagg tgccaagatc tatcccgata tacatatcga tcgttcctta
     1501 aaaaaatgcc cggtatcgct accgataacc gggccaacgg actgcacagt tagccgttat
     1561 ttgtcgaaca gataatgatt taccagattt tccagttgct cctggcgacc actctgatgc
     1621 accggagaca aattatgttc ctgagcatat ttggctaaat ctgccagtga catttggcct
     1681 ttcaggattt gctggcccaa ttcgctattc cagccggaat aacgctgcgc gatgcgttta
     1741 tccagctcgc catcttcaat catgcgcgct gcaattttca gcgccactgc catcgtatcc
     1801 atcgcgccga tatgaccgta aaacagatca tatttatcag tactttgacg acgtactttg
     1861 gcatcgaagt tcagaccacc ggtggtgaaa ccacctgctt tgagaatttc atacatcacc
     1921 agcgcattct cttccacact gttcgggaac tggtcggtgt cccagcccag ttgcgcatcg
     1981 ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa tggcggtggc tatttcatga
     2041 tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa tgttcagttt aatctctttt
     2101 tccagaccaa actgtttcag gaagccatag accgtcgcgg catcgtaatc atattgatgt
     2161 ttagtcggtt cttgcggttt cggttcgata agcaacgtgc cctggaagcc gattttatgt
     2221 ttatgctcaa ccaccatctg cataaagcgg cccagttgtt cacgctcctg acgcaagtcg
     2281 gtatttaaca gcgtttcgta accttcacga ccaccccaca ggacatagtt ttcaccgccc
     2341 aatttatggg ttgcttccat cgctgtaaca acttgcgttg ccgcccagct gaagacttca
     2401 ggatctgggt tcgtcgccgc acccgcgcca tagcgagggt ttgtaaagca gttagcggtt
     2461 ccccacagca gcttcacgcc gctctcttct tgcttggctg ccaggacatc aaccatttgc
     2521 gcaaaattat tgatgtactc ttttaacgac gcgccctcag gggaaacatc cacatcgtgg
     2581 aagcaataaa atggcacatg taacttgtgg aaaaactcaa atgcgacatc tgctttacgc
     2641 ttcgccaacg ccagtgcctc accaggctgc tgccacggac gattaaacgc ccccacacca
     2701 aacatgtccg ccccgttcca gcagaaggtg tgccagtagc aggcggcaaa acgcaagtgc
     2761 tcttccatac gcttacccaa caccagttcg tcgggattgt agtgacggaa tgctaacggg
     2821 tttgaggatt ttgagccttc ataacgaacg cgatcgagct ggtcaaaata ggcttgcata
     2881 ttgaactcca taatcaggta atgccgcggg tgatggatga tgtcgtaata ttgggcactc
     2941 cctttcagtt gctcaattat gttatttcac actgctattg agataattca caagtgtgcg
     3001 ctcgctcgca aaataaaatg ggatgatgaa actgggtaat tcctcgaaga taaaaatgca
     3061 ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata aatcaagaaa taaaccaaaa
     3121 atcgtaatcg aaagataaaa atctgtaatt gttttcccct gtttagttgc taaaaattgg
     3181 ttacgtttat cgcggtgatt gttacttatt aaagctgtcc tctaactaca gaaggcccta
     3241 caccatgaaa ataaagaaca ttctactcac cctttgcacc tcacttctgc ttaccaacgt
     3301 tgcggcacac gccaaagaag tcaaaatagg tatggcgatt gatgatctcc gtcttgaacg
     3361 ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa tctctcggcg cgaaagtatt
     3421 tgtacagtct gcaaatggca atgaagaaac acaaatgtcg cagattgaaa acatgataaa
     3481 ccggggtgtt gatgttcttg tcattattcc gtataacggt caggtattaa gtaacgttgt
     3541 aaaagaagcc aaacaagaag gtattaaagt attagcttac gatcgtatga ttaacgatgc
     3601 ggatatcgat ttttatattt ctttcgataa cgaaaaagtc ggtgaactgc aggcaaaagc
     3661 cctggtcgat attgttccgc aaggtaatta cttcctgatg ggcggctcgc cggtagataa
     3721 caacgccaag ctgttccgcg ccggacaaat gaaagtgtta aaaccttacg ttgattccgg
     3781 aaaaattaaa gtcgttggtg accaatgggt tgatggctgg ttaccggaaa acgcattgaa
     3841 aattatggaa aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa
     3901 cgatgccacc gcaggtgggg caattcaggc attaagcgcg caaggtttat cagggaaagt
     3961 agcaatttcc ggccaggatg cggatctcgc aggtattaaa cgtattgctg ccggtacgca
     4021 aactatgacg gtgtataaac ctattacgtt gttggcaaat actgccgcag aaattgccgt
     4081 tgagttgggc aatggtcagg aaccaaaagc agataccaca ctgaataatg gcctgaaaga
     4141 tgtgccctcc cgcctcctga caccgatcga tgtgaataaa aacaacatca aagacacggt
     4201 agttaaagac ggattccaca aagagagcga gctgtaagcg ttacgccccg gcgcggagcg
     4261 ggggcgtgat ttctctccat gccgcgtgaa tgaattggct taggtggagt cgttatgcct
     4321 tatctacttg aaatgaagaa tattaccaaa accttcggca gtgtgaaggc gattgataac
     4381 gtctgcttgc ggttgaatgc tggcgaaatc gtctcacttt gtggggaaaa tgggtctggt
     4441 aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc atggctccta cgaaggcgaa
     4501 attatttttg cgggagaaga gattcaggcg agtcacatcc gcgataccga acgcaaaggt
     4561 atcgccatca ttcaccagga attggccctg gtgaaagaat tgaccgtgct ggaaaatatc
     4621 ttcctgggta acgaaataac ccacaatggc attatggatt atgacctgat gacgctacgc
     4681 tgtcagaagc tgctcgcaca ggtcagttta tccatttcac ctgatacccg cgttggcgat
     4741 ttagggcttg ggcaacaaca actggttgaa attgccaagg cacttaataa acaggtgcgc
     4801 ttgttaattc tcgatgaacc gacagcctca ttaactgagc aggaaacgtc ggttttactg
     4861 gatattattc gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc
     4921 aacgaagtca aagcgatttc cgatacgatt tgcgttattc gcgacggaca gcacattggt
     4981 acgcgtgatg ctgccggaat gagtgaagac gatattatca ccatgatggt cgggcgagag
     5041 ttaaccgcgc tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa
     5101 catctgacgg catggcatcc ggtcaatcgt catattaaac gagttaatga tgtctcgttt
     5161 tccctgaaac gtggcgaaat actgggtatt gccggactcg ttggtgccgg acgtaccgag
     5221 accattcagt gcctgtttgg cgtgtggccc ggacaatggg aaggaaaaat ttatattgat
     5281 ggcaaacagg tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggta
     5341 cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc
     5401 ctcgccgcac tcaataaatt taccggtggc attagccagc ttgatgacgc ggaagagcaa
     5461 aaatgtattc tggaatcaat tcagcaactc aaagttaaaa cgtcgtcccc cgatcttgct
     5521 attggacgtt tgagcggcgg caatcagcaa aaagcgatcc tcgctcgctg tctgttactt
     5581 aacccgcgca tcctcattct tgatgaacct accaggggta tcgatattgg cgcgaaatac
     5641 gagatctaca aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct
     5701 tccgaattac ctgaagtgct cggccttagc gatcgggtac tggtgatgca tgaagggaaa
     5761 ctaaaagcca acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg
     5821 aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg aaattggccg taccgacatc
     5881 cggtggcttt tccgggctga aatcactgaa tttgcaggtc ttcgtgatga ttgcagctat
     5941 catcgcaatc atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa
     6001 cgtctccaac ctgttacgcc agaccgcgat taccggcatc ctcgcggtag gaatggtgtt
     6061 cgtcataatt tctgctgaaa tcgacctttc cgtcggctca atgatggggc tattaggtgg
     6121 cgtcgcggcg atttgtgacg tctggttagg ctggcctttg ccacttacca tcattgtgac
     6181 gttggttctg ggactgcttc tcggtgcctg gaacggatgg tgggtcgcgt accgcaaagt
     6241 cccttcattt attgtcaccc tcgcgggcat gttggcattt cgcggcatac tcattggcat
     6301 caccaacggc acgactgttt cccccaccag cgccgcgatg tcacaaattg ggcaaagcta
     6361 tctacctgcc agcactggct tcatcattgg cgcgcttggc ttaatggctt ttgttggttg
     6421 gcaatggcgc ggaagaatgc gccgtcaggc tttgggtttg cagtctccgg cctctaccgc
     6481 agtagtcggt cgccaggctt taaccgctat catcgtatta ggcgcaatct ggctgttgaa
     6541 tgattaccgt ggcgttccca ctcctgttct gctgctgaca ttgctgttac tcggcggaat
     6601 gtttatggca acgcggacgg catttggacg acgcatttat gccatcggcg gcaatctgga
     6661 agcagcacgt ctctccggga ttaacgttga acgcaccaaa cttgccgtgt tcgcgattaa
     6721 cggattaatg gtagccatcg ccggattaat ccttagttct cgacttggcg ctggttcacc
     6781 ttctgcggga aatatcgccg aactggacgc aattgcagca tgcgtgattg gcggcaccag
     6841 cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg ggggcattta tcatggcttc
     6901 actggataac ggcatgagta tgatggatgt accgaccttc tggcagtata tcgttaaagg
     6961 tgcgattctg ttgctggcag tatggatgga ctccgcaacc aaacgccgtt cttgattttg
     7021 ataaaaattt tctcaaagcc ggttacgtat taccggtttt gagtttttgc atgattcagc
     7081 aggaaaagaa ccatgtttac taaacgtcac cgcatcacat tactgttcaa tgccaataaa
     7141 gcctatgacc ggcaggtagt agaaggcgta ggggaatatt tacaggcatc acaatcagaa
     7201 tgggatattt tcattgaaga agatttccgc gcccgcattg ataaaatcaa ggactggtta
     7261 ggagatggcg tcattgccga cttcgacgac aaacagatcg agcaagcgct ggctgatgtc
     7321 gacgtcccca ttgttggggt tggcggctcg tatcaccttg cagaaagtta cccacccgtt
     7381 cattacattg ccaccgataa ctatgcgctg gttgaaagcg catttttgca tttaaaagag
     7441 aaaggcgtta accgctttgc tttttatggt cttccggaat caagcggcaa acgttgggcc
     7501 actgaacgcg aatatgcatt tcgtcagctt gtcgccgaag aaaagtatcg cggagtggtt
     7561 tatcaggggt tagaaaccgc gccagagaac tggcaacatg cgcaaaatcg gctggcagac
     7621 tggctacaaa cgctgccacc gcaaaccggg attattgccg ttactgacgc ccgggcgcgg
     7681 catattctgc aagtatgtga acatctacat attcccgtac cggaaaaatt atgcgtgatt
     7741 ggcatcgata acgaagaact gacccgctat ctgtcgcgtg tcgccctttc ttcggtcgct
     7801 cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt tgcatcgatt attagataaa
     7861 gaagaaatgc cgctacagcg aattttggtc ccaccagttc gcgtcattga acggcgctca
     7921 acagattatc gctcgctgac cgatcccgcc gttattcagg ccatgcatta cattcgtaat
     7981 cacgcctgta aagggattaa agtggatcag gtactggatg cggtcgggat ctcgcgctcc
     8041 aatcttgaga agcgttttaa agaagaggtg ggtgaaacca tccatgcaat gattcatgcc
     8101 gagaagctgg agaaagcgcg cagtctgctg atttcaacca ccttgtcgat caatgagata
     8161 tcgcaaatgt gcggttatcc atcgctgcaa tatttctact ctgtttttaa aaaagcatat
     8221 gacacaacgc caaaagagta tcgcgatgta aatagcgagg tcatgttgta gcgatgatga
     8281 gaattgtcgg cgtcacatca gggaactcag cacagccacc tgatgcgagc aaaagcctta
     8341 tccgaccgac aaatcagata aggctcaagg aaatgcaatt acatatgcgc cgcgattaac
     8401 cgttggttat cctggtacat tgcgaacagg tagttgttat aactcttccc tttggtcgag
     8461 tagcccttca gcttgtgaat catcgctgtg gcagtcactt cctgatccgc tttacgcagc
     8521 tgcgcacgcg atttacggaa tgaagagtaa gccggatgcg tattgaggtt agtgacatag
     8581 gcgctcaccg attctttgac agaactaaac tgtgagtacc ctttcacttt acccggcgca
     8641 ttggtacaac gtcctttcat gcatttcatg ccgaacagat tgttgttgtt gcgcgccagc
     8701 tttgacgttc cccaaccgct ttctgctgca gccatcgtcg ccaccatact ggtggggata
     8761 atgtctacgc gttcaagcaa ggtattccac gggatttttc gcgtattacc agaccacttc
     8821 accttgtagc gtttggcgat gtctttcaga cgcgcacgct cagcaggtga ccattggccc
     8881 tgatactgtt ttgaaatcag ccagttacgt tccgcagtaa tggctgcatt ttggctggta
     8941 atgtaaggca ttacggtccg gagaaacgcc tttttccttg gtgttccgga agggtatttt
     9001 cgcaaatcag gaagtgaatt actctttgca ctattgcgag aatactcttg tttactgctt
     9061 acctgtttat tactagcttt agttaagtgg gacttttgac tcgctgttgt tgtgtgcgtc
     9121 ttcgctaaca cctcactcga aaacaccaga gtgagtaaca taagaatcat cgccccatat
     9181 cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga tttaatcatt ccaacacctt
     9241 atatttttca caaatttgag agttgaatct caaatcatat caaaaatagc tgtcaagagc
     9301 accccaagga atagtccaaa tctgaaacta tgtcacgagt taacgattca gattggcgct
     9361 aaatcgcaga aaatgtgggg tttatcgcaa aattcagccg ttttttgcgc gagatcgctc
     9421 acccttgctt ctcatcctgt ggacttaccg ctcagggatg agttttgttt ggcttatcgc
     9481 tggcaaactg tctgaaatcg cagcaataag gactcatccg ccatgaaact cgccgcctgt
     9541 tttctgacac tccttcctgg cttcgccgtt gccgccagct ggacttctcc ggggttccct
     9601 gcctttagtg aacagggaac ggaaacattt gtcagccacg cgcagttgcc caaaggtacg
     9661 cgtccactaa cgctaaattt tgaccaacag tgctggcagc ctgcggatgc gataaaactc
     9721 aatcagatgc tttccctgca accttgtagc aacacgcagc ctcaatggcg attgttcagg
     9781 gacggcgaat atacgctgca aatagacacc cgctccggca cgccaacatt gatgatttcc
     9841 atccagaacg ccgccgaacc ggtagcaagc caggtccgtg tatgcccgaa atgggatgga
     9901 ttaccgctca cgctggatgt cagcgccact ttcccggaag gagccgccgt acgggattat
     9961 tacagccagc aaattgcgat agtgaagaac ggtcaaataa cgttacaacc cgctgccacc
    10021 agcaacggtt tactcctgct ggaacgggca gaaactgacg cctctgcccc tttcgactgg
    10081 cataacgcca cggtttactt tgtgctgaca gatcgtttcg aaaacggcga tcccagtaat
    10141 gaccagagtt acggacgtca taaagacggt atggcggaaa ttggcacttt tcacggcggc
    10201 gatttacgcg gcctgaccaa caaactggat tacctccagc agttgggcgt taatgcttta
    10261 tggataagcg ccccatttga gcaaattcac ggctgggtcg gcggcggtac aaaaggcgat
    10321 ttcccgcatt atgcctacca cggttattac acacaggact ggacgaatct tgatgccaat
    10381 atgggcaacg aagccgatct acggacgctg gttgatagcg cacatcagcg cggtattcgt
    10441 attctctttg atgtcgtgat gaaccacacc ggctatgcca cgctggcgga tatgcaggag
    10501 tatcagtttg gcgcgttata tctttctggt gacgaggtga aaaaaacgct gggtgaacgc
    10561 tggagcgact ggaaacctgc cgccgggcaa acctggcata gctttaacga ttacattaat
    10621 ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa actggatccg taccgatatc
    10681 ggcgattacg acaatcctgg attcgacgat ctcaccatgt cgctagcctt tttgccggat
    10741 atcaaaaccg aatcaactac cgcttctggt ctgccggtgt tctataaaaa caaaacggat
    10801 acccacgcta aagccatcga cggctttacc cctcgcgatt acttaaccca ctggttaagt
    10861 cagtgggtcc gcgactatgg gattgatggt tttcgggtcg ataccgccaa acatgttgag
    10921 ttgcccgcct ggcagcaact gaaaaccgaa gccagcgccg cgcttcgcga atggaaaaaa
    10981 gctaaccccg acaaagcatt agatgacaaa cctttctgga tgaccggtga agcctggggc
    11041 cacggcgtga tgcaaagtga ctactatcgc cacggcttcg atgcgatgat caatttcgat
    11101 tatcaggagc aggcggcgaa agctgtcgat tgtattgcgc agatggatat gacctggcag
    11161 caaatggcgg agaaattgca gggtttcaac gtgttgagct acctctcgtc gcatgatacc
    11221 cgtctgttcc gtgaaggggg cgacaaagca gcagagttat tactattagc gccaggcgcg
    11281 gtacaaatct tttatggcga tgaatcctcg cgtccgttcg gtcctacagg ttctgatccg
    11341 ctgcaaggta cacgttcgga tatgaactgg caggatgtta gcggtaaatc tgccgccaac
    11401 gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc atcccgcaat tggcgcgggc
    11461 aaacaaacga cactttcgct gaagcagggc tacggctttg ttcgtgagca tggcgacgat
    11521 aaagtgctgg tcatctgggc tgggcaacag tgacttttcc ggcttcccgt tcgtcagtac
    11581 ctcgggaagc cgccaaccag gataaaatgt cagccctaat cagcgttgca ggataaagca
    11641 ccgctcactc ttcaacagac cgatttgcac cccagcaaat gtagcgttat tgttaccttc
    11701 cttgctacag agttcgacag atatcccgct atgacattct ccctttttgg tgacaaattt
    11761 acccgccact ccggcattac gctgctgatg gaagatctga acgacggttt acgcacccct
    11821 ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca ggactacttc
    11881 cagacgctac tgaccgacat gctggaaagt ggcaaagcga ctgatgcact gtgcaattac
    11941 gacggtccac aggggaaaac ggagctactc acactgcttg ccggaatgct gcgcgagaag
    12001 ttgggttggg atatcgaacc acagaatatt gcactaacaa acggcagcca gagcgcgttt
    12061 ttctacttat ttaatctgtt tgccggacgc cgtgccgatg gtcgggtcaa aaaagtgctg
    12121 ttcccgctgg caccggaata cattggctac gctgacgccg gactggaaga agatctgttt
    12181 gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc agtttaaata ccacgtcgat
    12241 tttgagcatc tgcatattgg cgaagaaacc gggatgattt gcgtctcccg gccgacgaat
    12301 ccaacaggca atgtgattac tgacgaagag ttgctgaagc ttgacgcgct ggcgaatcaa
    12361 cacggcattc cgctggtgat tgataacgct tatggcgtcc cgttcccggg tatcatcttc
    12421 agtgaagcgc gcccgctatg gaatccgaat atcgtgctgt gcatgagtct ttccaagctg
    12481 gggctgcctg gctcccgctg cggcattatc atcgccaatg aaaaaatcat caccgccatc
    12541 accaatatga acggcattat cagcctggca cctggcggta ttggtccggc gatgatgtgt
    12601 gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa accgttttac
    12661 taccagcgtg ttcaggaaac tatcgccatc attcgccgct atttaccgga aaatcgctgc
    12721 ctgattcata aaccggaagg agccattttc ctctggctat ggtttaagga tttgcccatt
    12781 acgaccgagc agctctatca gcgcctgaaa gcacgcggcg tgctgatggt gccggggcac
    12841 aacttcttcc cagggctgga taaaccgtgg ccgcatacgc atcaatgtat gcgcatgaac
    12901 tacgtaccag agccggagaa aattgaggcg ggggtgaaga ttctggcgga agagatagaa
    12961 agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc tacgcttatc aggcctacat
    13021 gatttctgca atatattgaa tttggaagaa tttgtaggcc ggataaggcg tttacgccgc
    13081 atctggcata aacaaagcgc actattccgg cgttgagaaa cgccggaaaa acgtttcaaa
    13141 caggctgctg ccgtagccga tgccgctgca ctttctcgac atccatgcac tgcaacgcct
    13201 ttgtcgggca ggcttcaaca caggccggtc ccgtctcccg atgccagcac agatcgcact
    13261 taatcgccct cgcctttttg cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca
    13321 gcatacagct tttacagcca atgcaacgtg tttgttcaac gaaaatatgc ccatgttcgc
    13381 ggcttatcgc gtcaacaggg cagacattcg cgcacggtgc atcttcacac tgatgacagg
    13441 ctactgccgt ggtccagctg tggtctttaa tgacacgaat acgggaaata aactcgtctg
    13501 gtgacaacgc agcgcaatcc tgattctcct gatgcgacac tgcgcaagcc acttcacagg
    13561 tacggcaacc gatacatttc gtcgcatccg caataataaa ccggttcatg atcactcctt
    13621 tgcactaaaa cagcagagtg ccagagagcg cgtccctact gctttgatcc aacgaaggaa
    13681 atgttaaagt tttggcactt gtccaaagcg ttatgtgatc gcgcccaggt catccctgac
    13741 agtaaatccc agttcattag aaatagcctg cgcggtttca cgcagtggtt tcaggagatt
    13801 tttctctccc acctgtttca gacgtgatgt cgaaagcgaa atcgacacgg cgtacggcac
    13861 ccgcccatga atatcaaaca ccggaacagc aatacaggag acgccgagtt cgttttcttc
    13921 tctgtccatc gccgctccgc tttcacgaat gtgcgccagt tcgtcgaaca tcgcgggcag
    13981 ctcggtaatg gtattgcggg ttaacggctg gatctcatgc tgatggtttt cccagtatga
    14041 cttcacgtag tccgggtgac caaacgccat gtagatcttg cccattgcgg aacagtagag
    14101 cggcatatgc tggccaatat aggcacgggt tcgcagcatc ccggttgtgg gttccagctt
    14161 ataaatcaaa atagcgtgat cgtcttcgcg gctggagaag ttaatggttt caccagtggc
    14221 gatgttcagt gcctcaagat gcggagcggc gatatgaatg atattcagcg aagacagcgc
    14281 cttctgcccg acggcaataa atttggtagt caggcggtaa ctccctgcgg cgggcgcggt
    14341 ggtcacatag ccgcaggact gtaatccctg caataagcga tggacggtac tcttatttaa
    14401 accagccagc tccgaaagat gcgccaacgg acaaccgttt ggatagttgc tcaaaatctc
    14461 aatcagcatc aacccgcgaa acagactctg gcttccggct ggacgctctt tttcctgcgc
    14521 catctcgttc tctttttttc ccatcacttc tttccccatt ttgtcgcgtc ctgatggtag
    14581 cgcaaagtgt gccgtagttc acgatctcga cagataattt ataaccactt gatttttatg
    14641 tcttttgaaa ttcatcaatc agattgcttt gttaaaaagt gatcgatata tttgaaatca
    14701 agtttcgcat attgaaattt taagccaaaa aagcgatcaa aaaaacaagg aagcctggga
    14761 tgaaagtgac atttgagcag ttaaaagcag cctttaatcg ggtcttaatt tcacgcggcg
    14821 ttgacagcga aacggctgac gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg
    14881 tttattctca cggcgttaat cgtttccctc gtttcattca acaactggaa aacggcgata
    14941 tcattcctga tgcccaaccc aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg
    15001 cccagcgttc gatcggcaac ctgacggcga aaaagatgat ggatcgcgcc attgaactgg
    15061 ctgccgatca cggtattggt ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg
    15121 gcagctacgg ctggcaggcg gcggaaaaag gctatattgg catttgctgg accaactcca
    15181 tcgccgtaat gccgccgtgg ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg
    15241 tcgccattcc ttccacgccg atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg
    15301 gcatgttaga agttaaccgc ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg
    15361 atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa gaatcgccgc attttgccga
    15421 tgggctactg gaaaggttct ggcatgtcga ttgtgctgga tatgatcgct actctccttt
    15481 ccgacggcgc atcggttgcc gaagtcaccc aggacaacag cgacgaatac ggcatttcac
    15541 aaatttttat tgccattgaa gtggacaagc ttatcgacgg tcccacccgc gatgccaagc
    15601 tgcaacgtat catggattac gttactaccg ctgaacgcgc tgacgaaaac caggccatcc
    15661 gcttacccgg ccacgaattt actaccctgc tggccgaaaa ccgccgtaac ggtattaccg
    15721 ttgatgacag cgtgtgggcc aaaatccagg cgttataagg agataagtca tgatttttgg
    15781 acatatcgcg cagcccaatc cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt
    15841 tctgcgcgct accgatttca acgccctgga gccgggcgtt gtcgaaatcg acggcaaaaa
    15901 tatttatgcg caaattatcg atttaaccac acgcgaagcg gtggaaaacc gtccggaagt
    15961 gcatcgtcgg tatatcgata ttcagtttct ggcgtggggt gaagagaaaa tcggcattgc
    16021 tattgatacg ggaaataata aagtcagcga atcactatta gagcagcgcg atattatttt
    16081 ttatcatgac agtgaacatg aatcatttat agaaatgata ccgggcagct acgcaatttt
    16141 ctttccgcag gatgttcacc gaccaggttg tattttgcaa acagcctctg agatacgaaa
    16201 aattgtggtt aaagtcgcat taacggcgct gaattaatcg aataatcgtc tacgccagaa
    16261 cgactgggta atgttattgc tctaactata ttaataattt aggttgctgc acatgcaact
    16321 tgaattatga cagacatagc ctcaaggaat agctatgaaa aaaatacttg aagcaatact
    16381 ggcgattaat ctcgccgtac tttcctgtat tgtgtttata aacattattt taagatatgg
    16441 ttttcagaca agcattttat ctgtagatga attgtcacgt tatttatttg tctggttaac
    16501 atttattggc gcgattgtgg cttttatgga taacgcccac gttcaggtca cctttctggt
    16561 ggagaaactc tcccccgcat ggcaacgacg agtcgctctg gttacccatt ccttaatctt
    16621 atttatttgt ggcgcactgg cctggggcgc aacgctaaaa acaattcagg actggagcga
    16681 ttattcaccg atcctcggtt tacccatcgg cctgatgtat gccgcttgtc tgcccaccag
    16741 cctcgttatc gcattctttg aactgcgtca tttatatcaa ctcatcacgc gtagcaattc
    16801 tctaacgtcg ccaccgcaag gagcttaatc atggctgtgc tgatttttct gggctgtctg
    16861 ttgggtggta ttgctatcgg tttgcctatt gcctgggcac tgttgttgtg cggggcggcg
    16921 ttaatgttct ggctggacat gtttgatgtc cagatcatgg cacaaacgct ggtgaacgga
    16981 gccgatagct tctccctgct ggcgattccg ttctttgtgc tggcgggtga aatcatgaat
    17041 gcgggcggct tgtcaaaacg cattgttgac ctgccgatga aactggtggg gcataaacca
    17101 ggcgggctgg gctacgtcgg cgtgctggca gctatgatta tggcgagcct ttctggttct
    17161 gccgttgcgg ataccgccgc cgttgccgct ctactggtgc cgatgatgcg cagcgccaac
    17221 tacccggtca accgggcggc ggggctgatt gcctctggcg gcattatcgc gccaattatt
    17281 cccccttcca ttccgtttat tatcttcggc gtttccagcg gattatccat cagtaagctg
    17341 tttatggcgg gcattgcccc tggcatgatg atgggcgcaa cgctgatgct tacctggtgg
    17401 tggcaggcca gtcgccttaa tttaccccgc cagcaaaaag caacgatgca ggaaatctgg
    17461 cactcttttg tctccggtat ctgggcgctg tttcttccag tcatcattat tggcgggttt
    17521 cgctccggtc tgtttacccc taccgaagcc ggggccgttg ctgctttcta tgcgctgttt
    17581 gtcgccacag ttatttaccg tgaaatgacc ttcgccacac tttggcatgt actcattggc
    17641 gcggcgaaaa ccacctcagt ggttatgttt ctggtggcct cggcacaagt ttccgcctgg
    17701 ctgattacca ttgctgaact gccgatgatg gtttctgatt tactgcaacc gttggtcgat
    17761 tcaccgcgtc tgctgtttat cgtcattatg gtggctatcc tgattgtcgg catggtcatg
    17821 gatttaacgc cgacggtatt aattcttacc ccggtattaa tgcctttagt taaagaggca
    17881 ggaatcgatc cgatttattt cggtgtcatg tttatcatta actgctcaat cggcttaatt
    17941 acaccgccta tcggtaatgt acttaacgtt atttcagggg tggcaaaact caaattcgat
    18001 gatgcagtca gaggcgtttt cccttacgtc ctggttttat attcattatt agtcgtattt
    18061 gtttttattc ccgatctcat catcctccct ttgaaatgga ttaattaaaa ggaaaatatt
    18121 atgaaattac gctctgtaac ctacgcatta ttcattgctg gcctggctgc attcagcaca
    18181 tcttctctgg cggcgcaatc tttacgtttc ggttatgaaa cctcacaaac cgactcgcaa
    18241 catattgcgg cgaaaaaatt caatgattta ttgcaggaga gaaccaaagg cgagctgaaa
    18301 ttaaaattgt ttccggatag caccctcggt aacgcgcagg cgatgatcag cggcgtacgt
    18361 ggcggcacca tcgatatgga aatgtccggc tcgaataact ttgccggatt atcaccagtg
    18421 atgaacttgc ttgatgtccc tttcctgttc cgcgataccg ctcacgcgca taaaacgctc
    18481 gacggcaaag tcggtgatga tctgaaagcc tcacttgaag gtaaaggact aaaagtactg
    18541 gcctactggg aaaacggctg gcgcgatgtc accaactcgc gcgcaccggt taaaaccccc
    18601 gccgacctga aagggctgaa aattcgcacc aacaatagcc cgatgaatat cgccgcattc
    18661 aaagtctttg gcgctaaccc gatcccgatg ccgtttgccg aagtctatac cgggctggaa
    18721 acccgcacta tcgacgctca ggaacacccg atcaacgtcg tctggtcagc aaaatttttc
    18781 gaagtgcaga agtacctttc tctgacgcac cacgcctatt ccccgcttct ggtggtgatc
    18841 aacaaagcga agtttgatgg cttaagcccg gagttccagc aagcgctaat ttcatctgcg
    18901 caggaagcgg gtaactacca gcgcaaactg gttgccgaag atcagcaaaa aatcatcgac
    18961 ggcatgaaag aagcgggcgt ggaagtcatc accgatctcg accgcaaagc ctttagcgac
    19021 gcactgggta ctcaggtccg cgacatgttt gtaaaagatg ttccgcaggg tgctgatcta
    19081 ctgaaagccg tggatgaggt gcaataaacc atgacgcaat actggctggg gttagattgt
    19141 ggcggtagct ggctgaaagc cgggctgtat gaccgcgaag gccgggaggc aggcgtgcag
    19201 cgcctgccgc tgtgcgcatt aagcccgcag ccaggttggg cagagcgcga tatggcagaa
    19261 ctgtggcaat gctgcatggc tgtcattcgc gaactgctta cccattccgg cgttagcggg
    19321 gaacaaattg tcggtatcgg tatctccgca caaggaaaag gcttgttttt gctggataaa
    19381 aacaacaagc cgctcggtaa cgccatttta tcctcggatc gccgggcgat ggaaatcgtt
    19441 cgtcgctggc aggaagatgg catcccggaa aaactctacc cgctgacccg acaaaccttg
    19501 tggaccgggc atccggtatc gctgttacgt tggcttaaag agcacgaacc ggaacgctac
    19561 gcgcaaattg gctgcgtgat gatgacgcac gactacctgc gctggtgttt aaccggcgtc
    19621 aaaggctgtg aagagagcaa tatttccgag tccaatctct acaacatgag tcttggggaa
    19681 tatgacccgt gcctcaccga ctggctgggg attgctgaaa tcaatcacac cctgccgcct
    19741 gttgtcggat ctgccgaaat ctgcggggag atcaccgctc agacagccgt actgaccggt
    19801 ctgaaagcgg gtacgcccgt tgtcggcggc ctgtttgatg tggtttccac cgcactctgc
    19861 gccgggatcg aagacgaatt tacccttaat gcggtgatgg gcacctgggc ggtgaccagc
    19921 ggcataaccc gcggtttacg tgacggtgaa gcgcatccgt atgtctatgg tcgctacgtt
    19981 aacgatggtg aatttatcgt tcacgaagcc agccccacct cttccggcaa cctcgaatgg
    20041 tttaccgcac agtggggaga aatctcgttt gatgagatca accaggccgt tgccagcttg
    20101 ccgaaggccg gtggcgatct ctttttcctg ccgttcctgt acggcagcaa cgccggactc
    20161 gagatgacca gcggtttcta cgggatgcag gccattcaca cccgcgcaca cctgttgcag
    20221 gccatttatg aaggggtggt gttcagccat atgacccatc tcaaccggat gcgcgaacgt
    20281 tttactgatg tgcacaccct gcgcgtcact ggcggcccgg cgcattccga tgtctggatg
    20341 caaatgctgg cggacgtcag cggtctgcgt attgagctgc cgcaggtgga agaaaccggc
    20401 tgctttggtg cagcacttgc cgcccgcgtc ggcaccgggg tttatcgcga tttcagcgaa
    20461 gcccagcgtg atttacagca cccggtgcgc accctgctgc cggatatgac tgtccatcag
    20521 ctttaccaac aaaaatacca acgctatcag catctcattg ccgcacttca gggctttcac
    20581 gcccgcatca aggagcacat attatgagcc gaccacttct gcaactggca ctcgaccact
    20641 catcacttga agccgcacag cgcgacgtga cgcagttaaa agacagcgtc gatattgtcg
    20701 aagcgggcac cattctctgt ttaaacgaag ggcttggcgc ggtgaaagcc ttgcgcgaac
    20761 agtgccctga caaaatcatc gttgccgact ggaaggtcgc cgacgctggt gaaacgctcg
    20821 cgcaacaggc atttggcgca ggcgctaact ggatgaccat catctgcgcc gcaccgctcg
    20881 cgacggtgga aaaaggccac gcgatggcgc aacgctgcgg tggtgaaatt cagatcgagc
    20941 tgttcggtaa ctggacgctg gacgacgccc gcgactggca tcgtattggc gtgcggcagg
    21001 cgatttatca tcgcggtcgt gatgcacagg ccagcgggca acagtggggc gaagccgatc
    21061 tggcacgcat gaaggcgctt tccgatatcg gccttgagct ttccattacc ggcgggatta
    21121 cccctgctga cctgccgctg tttaaagata ttcgcgtgaa agcgtttatt gccgggcgag
    21181 cactggcagg ctcagcaaat ccggcgcagg tggctggcga tttccatgcg caaatcgacg
    21241 ctatctgggg aggcaagcgt gcgtaatcat ccgttaggga tttatgaaaa ggcgctggcg
    21301 aaagatctct cctggccgga gcggctggtg ctggcaaaaa gctgcggttt tgattttgtc
    21361 gaaatgtcgg tggatgaaac cgacgaacgg ctttcacgtc ttgactggag caccgcacaa
    21421 aggacttcgc tggttgccgc gatgatcgaa acgggtgttg ggatcccgtc gatgtgcctg
    21481 tccgcacatc gtcgctttcc ttttggtagc cgtgacgacg cggtacgcca gcgagcacgg
    21541 gagatcatga gcaaagcgat tcgtctggca cgcgatctcg gcatacgcac catccagctg
    21601 gcgggctacg acgtctatta cgaagaccac gatgaaggca cccggcaacg ttttgctgaa
    21661 gggctggcgt gggcggtcga acaggcggcg gcatcacaag tcatgctagc ggtggagatt
    21721 atggacaccg cgtttatgaa ctccatcagt aaatggaaaa agtgggacga gatgctcgcc
    21781 tcgccgtggt tcaccgtcta cccggacgtc ggcaacctca gcgcctgggg caatgatgtt
    21841 cccgccgaac tgaaactggg cattgaccgt atcgccgcga tccacctgaa agacacccag
    21901 ccagttaccg agcaaagtcc cggacagttc cgcgacgtgc cgtttggcga aggctgcgtc
    21961 gatttcgttg gcatcttcaa aacgctgcat aaactgaact atcgcggttc tttcctgatt
    22021 gagatgtgga ccgaaaaagc caaagagccg gtgctggaga ttattcaggc gcggcgttgg
    22081 attgaagcgc gtatgcagga ggctggattt atatgttaga gcaactgaaa gccgacgtgc
    22141 tggcggcgaa tctggcgctt cccgctcacc atctggtgac gttcacctgg ggcaatgtca
    22201 gcgcggtaga cgaaacgcgg caatggatgg taatcaaacc ttccggcgtc gagtatgacg
    22261 tgatgaccgc cgacgatatg gtggtggttg agatagccag cggcaaggtg gtggaaggta
    22321 gcaaaaagcc ctcgtccgac acgccaacgc atctggcgct ttaccgtcgc tacgccgaaa
    22381 tcggtggcat tgtgcatacc cactcacgcc acgccaccat ctggtcgcag gccgggctgg
    22441 atctccccgc ctggggcacc acgcacgctg attatttcta tggtgccatc ccctgcacgc
    22501 gacagatgac cgcagaggag attaacggcg aatatgaata tcagaccggc gaagtgatca
    22561 ttgaaacctt cgaagaacgt ggcaggagtc cggcacaaat cccggcggtg ctggtgcatt
    22621 ctcacggccc gttcgcatgg ggtaaaaacg ccgccgatgc cgtgcataac gccgtagtac
    22681 tcgaagaatg cgcctatatg ggcctattct cgcgccagct tgcgccgcag ctccctgcga
    22741 tgcaaaacga actgctggat aagcactacc tgcgtaagca tggggccaat gcctattacg
    22801 ggcagtaatc cctcacgccg gggcttcatc gccccggcac tacgaattga tatgttcctt
    22861 gctgtaacgc cgcttccacg ctgctggcgt taaaccggtg tgcttgcgaa aaatctgccg
    22921 gaaatagcca acgtcattaa accgtattgc atggagcagg ccgacaacgt cgagtcactg
    22981 cataacctgt tgctgcccgc cgatgcgcca tttacgacat ttgaaggcaa gggattgttc
    23041 agccataaga tcctgattca gacgccaggt tcccggtatg aacagagcaa tccagagcaa
    23101 caacctttgt ggcattacga cggagcacca gccgcaccga catccaccgg tgaattaaaa
    23161 acgccagctc acgccggtga taaaggtaaa actctcatta cgatcaatca tcgggctgtt
    23221 ggtaatctct tccggcagct cgctgtatcc tgcgctcgcc atcaatacga cgtgctctcc
    23281 tatcgggtat tttgccgtca ggctgacata gggcacccag gcatcctgcg cggaataact
    23341 tgccagaccg ctacgacggg actcactctc tgaaatgcca tagtaatagt cactgaaatt
    23401 ctcgtcataa tagagaacgc ccagcgcagg tgtcagcgac aggcgaccta tctgcatttt
    23461 gtggaatacc gatagctccc ccacccagcc gttgctgtta tccagaacgt ccgcagccgc
    23521 agaggctttc acggttcccc aacgctcgtg gtgataccag gcaacccccg ccatagccgt
    23581 actatcacgc ttatcaagct gttgcatggc ataatcgtca ttatcggcag ggtcaaattc
    23641 cagcggcatc caggatgcgg tcaggctaag ttcatttttt tcactttgcg acagaataaa
    23701 acctaatgtg gtctgacgaa cataaaaaga atcaccttca taactaatca gcggaattgc
    23761 ctttgtattt tcattataac cgcgataagg cgattcatta taagctgcac cagcaccaat
    23821 ggataattca gaagcagttg cgcttgccat aaaaggcaac gcaaataacg ccacaatatt
    23881 gcgattaatt aacatgtccg gtattccatt ttaaaataag ataaaaaaga gtcggcataa
    23941 taatgcttac aatttctccg gtg
//
LOCUS       DAZFKM010000072        23842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFKM010000072 DAZFKM010000000
VERSION     DAZFKM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..622
                     /gene="espM1"
                     /locus_tag="ACHKAY_002927"
     CDS             32..622
                     /gene="espM1"
                     /locus_tag="ACHKAY_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO5701581.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNSTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
     gene            complement(841..1897)
                     /locus_tag="ACHKAY_002928"
                     /pseudo
     CDS             complement(841..1897)
                     /locus_tag="ACHKAY_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2129..2779
                     /locus_tag="ACHKAY_002929"
     CDS             2129..2779
                     /locus_tag="ACHKAY_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5701582.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            2950..3185
                     /locus_tag="ACHKAY_002930"
                     /pseudo
     CDS             2950..3185
                     /locus_tag="ACHKAY_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3595..3696)
                     /locus_tag="ACHKAY_002931"
                     /pseudo
     CDS             complement(3595..3696)
                     /locus_tag="ACHKAY_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3920..4045
                     /locus_tag="ACHKAY_002932"
                     /pseudo
     CDS             3920..4045
                     /locus_tag="ACHKAY_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(4105..4611)
                     /gene="yciE"
                     /locus_tag="ACHKAY_002933"
     CDS             complement(4105..4611)
                     /gene="yciE"
                     /locus_tag="ACHKAY_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5701583.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(4657..5157)
                     /gene="yciF"
                     /locus_tag="ACHKAY_002934"
     CDS             complement(4657..5157)
                     /gene="yciF"
                     /locus_tag="ACHKAY_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO5701584.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5243..5422)
                     /gene="yciG"
                     /locus_tag="ACHKAY_002935"
     CDS             complement(5243..5422)
                     /gene="yciG"
                     /locus_tag="ACHKAY_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO5701585.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(5803..6609)
                     /gene="trpA"
                     /locus_tag="ACHKAY_002936"
     CDS             complement(5803..6609)
                     /gene="trpA"
                     /locus_tag="ACHKAY_002936"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO5701586.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(6609..7802)
                     /gene="trpB"
                     /locus_tag="ACHKAY_002937"
     CDS             complement(6609..7802)
                     /gene="trpB"
                     /locus_tag="ACHKAY_002937"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO5701587.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(7814..9175)
                     /gene="trpCF"
                     /locus_tag="ACHKAY_002938"
     CDS             complement(7814..9175)
                     /gene="trpCF"
                     /locus_tag="ACHKAY_002938"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO5701588.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(9176..10771)
                     /gene="trpD"
                     /locus_tag="ACHKAY_002939"
     CDS             complement(9176..10771)
                     /gene="trpD"
                     /locus_tag="ACHKAY_002939"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO5701589.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(10771..12333)
                     /gene="trpE"
                     /locus_tag="ACHKAY_002940"
     CDS             complement(10771..12333)
                     /gene="trpE"
                     /locus_tag="ACHKAY_002940"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO5701590.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(12355..12448)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(12425..12469)
                     /gene="trpL"
                     /locus_tag="ACHKAY_002941"
     CDS             complement(12425..12469)
                     /gene="trpL"
                     /locus_tag="ACHKAY_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO5701591.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            12607..13488
                     /gene="rnm"
                     /locus_tag="ACHKAY_002942"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             12607..13488
                     /gene="rnm"
                     /locus_tag="ACHKAY_002942"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO5701592.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            13485..14105
                     /gene="yciO"
                     /locus_tag="ACHKAY_002943"
     CDS             13485..14105
                     /gene="yciO"
                     /locus_tag="ACHKAY_002943"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO5701593.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            14206..15078
                     /gene="rluB"
                     /locus_tag="ACHKAY_002944"
     CDS             14206..15078
                     /gene="rluB"
                     /locus_tag="ACHKAY_002944"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO5701594.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(15118..15708)
                     /gene="cobO"
                     /locus_tag="ACHKAY_002945"
     CDS             complement(15118..15708)
                     /gene="cobO"
                     /locus_tag="ACHKAY_002945"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO5701595.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(15705..16463)
                     /gene="yciK"
                     /locus_tag="ACHKAY_002946"
     CDS             complement(15705..16463)
                     /gene="yciK"
                     /locus_tag="ACHKAY_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO5701596.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16683..17732
                     /gene="sohB"
                     /locus_tag="ACHKAY_002947"
     CDS             16683..17732
                     /gene="sohB"
                     /locus_tag="ACHKAY_002947"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO5701597.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(17768..18019)
                     /gene="yciN"
                     /locus_tag="ACHKAY_002948"
     CDS             complement(17768..18019)
                     /gene="yciN"
                     /locus_tag="ACHKAY_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO5701598.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            18399..20996
                     /gene="topA"
                     /locus_tag="ACHKAY_002949"
     CDS             18399..20996
                     /gene="topA"
                     /locus_tag="ACHKAY_002949"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO5701599.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            21206..22180
                     /gene="cysB"
                     /locus_tag="ACHKAY_002950"
     CDS             21206..22180
                     /gene="cysB"
                     /locus_tag="ACHKAY_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO5701600.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            22499..22639
                     /gene="ymiA"
                     /locus_tag="ACHKAY_002951"
     CDS             22499..22639
                     /gene="ymiA"
                     /locus_tag="ACHKAY_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO5701601.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            22642..22809
                     /gene="yciX"
                     /locus_tag="ACHKAY_002952"
     CDS             22642..22809
                     /gene="yciX"
                     /locus_tag="ACHKAY_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO5701602.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            22923..23018
                     /gene="ymiC"
                     /locus_tag="ACHKAY_002953"
     CDS             22923..23018
                     /gene="ymiC"
                     /locus_tag="ACHKAY_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO5701603.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            23182..>23842
                     /locus_tag="ACHKAY_002954"
     CDS             23182..>23842
                     /locus_tag="ACHKAY_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO5701604.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
BASE COUNT         6028 a         5647 c         6039 g         6128 t
ORIGIN      
        1 tgtaagcaaa ttttaatgaa aaggatttgt tatgccagta aatgcgacag gtgtgtcctt
       61 cagctctttt ggtatcagtt atcataaaga taattctttc cggggcacca tccgtgggaa
      121 gaatgatgag gtcgtgaagt gttcaatggg agagcgctct atccgcttta atgttaacaa
      181 atttagcggc tgtatactgg agacggtaag caggcagagt actaaagata ttcatggatg
      241 ggtaagtgat gaacggacag tatatccgtc aagggttatc aaccaagaga ttgataattg
      301 ctgccttcaa aaaaatgcaa aaatttcttc tgaagagagg aaaatggtct tttctcttgt
      361 aagtaaggag tttgaactaa ctcttgatgt taaagcggca caaagttcta ttaatcatat
      421 aataatagga aatgcttctt ttggcaaaaa aatggatgct ctttgcgatg gtatgagccg
      481 agctgtaaaa aacagtacaa cagattacat agcaaacgtg cttgcagaca agttttatca
      541 gaaacatatc gccccgggtg ttgatattgt aaaactacga aatgaaatcc caggttatat
      601 gagtcgtgtt atacaggggt aaggttggag gaggctatgc gccagtatca ttggttttca
      661 tcctgtaaaa agatatgagt aaaatataca acaggcgtta cactatatgt atgtatccaa
      721 ataactcata gttcacctgt acagttataa atcgtaaaga aaattgcagc gcgtcgtacg
      781 cagaaacgtg ctgtgattga ctatttatag ctttgtcaca acgacaagat tcagtagtgt
      841 ttacacgggc gggagaaatg acggaataac taaatagctg cgcctaatat cgcacatttt
      901 tgccagccca tgtttgcctt cggggaatgg actgacggat tccataaaat ggcgaacttt
      961 ttcaatagtt gccacattga gcggcactga tgattacgcg ttattgtggc gtgaagtatc
     1021 tgccatagct attcaaaatg attcacccat ggagattaaa ccggctgata aatgactctg
     1081 cacttcggtt tatccttcag ccagcgctgg gtttcccggc ctttgtggat aatgtagttg
     1141 tcctcgagca gcgtgatggt tttcgtccga cagtatgtag ctttaagccg cttcagcagt
     1201 ctgatgaaca gcgccgaact tttgctgttg gcgcccacac agctgactta acctgtcccg
     1261 ctgtgcagcg ctacgtccag agaatatttt tcattttgtt ccggcgtgac ccctcgtttt
     1321 tgcttcccga gcagtttcca gtccgcaccg attttgggat taagatggat aaatacttca
     1381 tcttcataaa agaccggatg ctctgtgctg cattcgtcca gtgcgttatg gattgttgcc
     1441 atctttttat ctttaggcgg ggcacggata cgtagagttg gcgcagccct tcgccacaca
     1501 agccccacag acgacaacca gcggcgaatg gttccagcat gtaacaggca gccggttatc
     1561 tcattgattt ttattgtcag cagttctgta ctccagcgtg aataattgta gccaaagtcg
     1621 cgagtgaata ttttacctgt tcacgtaata gtgtgtagat atgcgcaaac ggccagcggc
     1681 gggctcgctc ggcaggtaat gatttcagtc cctcaacacc cgactgtgtg aactagttaa
     1741 tccagcgccc aacagaggaa cgggcgcagc atagtgtgcg ggcaacgtcg ctgacacggt
     1801 cgccccggtg cagcatcagc atggctgtca gccttttatg gatggctttc tgtattaggc
     1861 gtcgttcgtc acgggaaatt ggtggtatga tcgacattgc tcagtccggt tggtgatttg
     1921 tattgatttg gcgattgatc agatcgcaca atcgggttga gttccctcta aatgatctac
     1981 tattctgcgc agctatttag tgagtatata ttttatgctt ataaattaga ttaaaataat
     2041 cctacaatta ttctgatgga gtattgctgt ggaggatggc tttttgttaa ttatcgaaaa
     2101 gtaagatata tgctttaggg ataaaattat gccattttca tttaatttgt cttctggaaa
     2161 ttacttgtca acacaagatg ttgaagtttt acaacgtgcg accagagatc atcaaatgga
     2221 aagactcaca ataggagaaa gatcgttttc tgttcgctat cagagtgcta tggacgcttt
     2281 tattgtagat cctgttcaag gggaactata ttcgggttta agccatacag aactagccga
     2341 tatcattaga ttggctgatt ctgttgaaaa tcaattgaat ggaggcaatt catttcttga
     2401 tgtattcagt acatatatgg ggcaggttat ttctgaattt atgcatagta atgataacag
     2461 aattgaattg ttacagcggc gattacattc atgttcattt ttagttaata ttgaagaaat
     2521 gtcttacata gatgaagcat tacagtgccc gattacgctg gcaattcctc aacgaggtgt
     2581 ttttttaaga aatgctgaag gttccagagt atgtagttta tatgatgaaa tggctctttc
     2641 tcgtataatt aatgatggga tgcatcaccc actaagcaga gagccaataa cattatcaat
     2701 gcttgtggcc agagagcagt gtgagtttga ttgcagtatc ggtcacttta cggtgaggag
     2761 tgattgttat tcagtgtaga ctcggtacac gattttgtgt gaagagactc ttattaaaag
     2821 ttagcatcaa agcggtcatc aaacttgata ataaagtgac tcattgctat tcgctggtcc
     2881 ttaagggata ttgcccattt tgtatcgtga actgaatagc aagtagccac cgtcctgatt
     2941 tcaacgcatt atcaggcgct tctgaagcgg catacctgca tgacaggatg agtgcaaagg
     3001 ggcgctgtta cgataatgct agggcggaaa gtttcttcca ctcactaaaa gtcgaatgca
     3061 tttacggcta atattttatg agtcgggaaa taatgcagac ggctgtgttt aattagatct
     3121 agtgtgatta caatcggtgg tgccgacaca gtacttgtgg ctgtctcagc acggaactgt
     3181 tttgaaaaac agaaccttga ttagggctgt gtccacatta cgtaggtagg atcaccccac
     3241 aggcaccaca tgtcatgcat ggattaggat tgaatgataa atcgtaaaga aaactgcaac
     3301 acctcgcgtg tagcacctgg ttagcgaact ttcgaaagta cgagtattgt atgatttcca
     3361 gcagttatca attttacgta ttaatcagtg aatcaacaac tcgtcagcag actctctttg
     3421 ctgttgaatt ttttggaacg tgtatagggc agaaattttt gttttgggac atggttggga
     3481 cgccgtagta catacgaact ctgccgaata acgtatgata ttatttttta atctcttgga
     3541 atacattata gaaacagaat gctcttgggc gatttttagt gattgtttaa atagccctgt
     3601 catgcagtta aagtggcggg catcctcttc aaggatggtg atcctcaacc gcacccattt
     3661 aggatgcgac tattgtggta gaacaatgta aaccataatt aatctgcgtg cggatacatg
     3721 cttcatagat aggatattct ggtttgggtt gtgaatgtgt tggtcatcga gtggacaata
     3781 aggtaagagg agtaactcct gcaaaacaaa aaaagagttc aacaaaatca atcagttaaa
     3841 aacacgagag atctgtataa aaaagcatcg taaattctgt atttttcctt taaatataat
     3901 atgttattga gttttgtaaa atctaccagt gtggtacatg gatatcgata ccaccgccaa
     3961 ttataagcta ggcggtgcac agcaacacga tagcgtacgc ctcgatccgt gggtgtttat
     4021 gttctcagca ggatatcgtt tttaattccg cacaaaaacg accccgtaat atacggggtc
     4081 aataaggaca tggtataaag ggtattattt cttcgcttct acgccatcag tttcagaacg
     4141 aattaaaaat ttctcagttg tttgcggaat atgctgaatc agccagtcgg ccatttgctt
     4201 ttcctcattt aaaatcgctt cgatgatcgg aattgagact gtatcaccgg cattttttgc
     4261 tgctgctaat agtgaggtgt aacaggcgat ttcaaattgc tcgaagacat atccgctaat
     4321 agagccttta actatttcat cagaagggaa tataccaccg attgactgcc caagcgcagc
     4381 cattttactc atggaatctt taatgactga acgtgaaatg tcattacgat caagaatagt
     4441 ttccagttga acaatctggt ttttggtttc actaagatgt tgttcaatac gagcgcgtag
     4501 ttcaggataa ttatctatac ggctggccat ggattcaagc atagattcgg cttgcttttc
     4561 cattgcgtgg gcgtcacgta accagtcatg ataatgttca atacgattca tgaaaatgtc
     4621 ctctttataa aatgactaaa agttaaaatt catatttcag gctttatttt cggctttctt
     4681 atttacgtta ttaagggcca gatcagtcag tttgatgtcg gtggcctttt cttcttccag
     4741 ggtttctttc agaagcttcg ctgctttacg gtaacctaat tgttcagcca gcgtcgctaa
     4801 tgtcccgtaa ctggcaatct cataatgctc gactttctgc gctgcggcaa tcagtgcggc
     4861 atcacgcact tcgtttttct cggtactttc gatgacctca ttagcttctt caataagacc
     4921 ttccattgcc acacatttca tgcgcttaat tttcaggttc gattcggact ccacaacttg
     4981 atcaatacgt tcaatctgtc catgagtttc ctcgaggtgc gcatgaaaag cctgacttaa
     5041 tttttcattt gatgttgctc ttgcgagttt tgccagtgcc cgggttaatt gtttttctgc
     5101 gctgtaggta tctgaaagca ggtgaataaa tacatcttca atggtcttca tattcatatt
     5161 tttctccagt gaaatcactg cgagccatac ggcccgcaga tgcaattatc tttatcagct
     5221 gatattaata attaacggtg aatcaggatt tgcctgattt attaccaccg ctttgttgac
     5281 cgcctttttt acccgcttca gatgcgcgtt gcggatcatt tttaaaatta ccgccgctat
     5341 gctgaccgcc tttacggcct gcgtcggatg ccttctcacg gtcttcggcg aaatttcctg
     5401 aaccaccacg atgttcggcc atgttatttc tcccgttgcg ttgcattgtt tcattaatat
     5461 gagtgttgtg tgtcgacact cattaaaatt agtcgctaat gagaattagt caaattaagc
     5521 gcaacgagaa gatagaggga aaatatattt tgaggaacat tctggatata ttaacaatta
     5581 cctgaggaat aagtgactta gagaaaatta ttgattttac tggtgttatg ttgcgggatt
     5641 aatttgtttg attatattac tgttgggcgg aaaaatgacg taagttgacg ctcgaccggg
     5701 gtaagcgaaa cggtaaaaag ataaatatta aatgaattta ggatttttcc ggcttcatta
     5761 aagaaagtta aaatgccgcc agcggaactg gcggctgtgg gattaactgc gcgtcgccgc
     5821 tttcatcggt tgtacaaaaa ctttcagtgc cgccagcatt ttctctggct cattaatatg
     5881 ttgctcgatg attttaacaa tggccgaacc agaaatcgcg cccgcagctc ctgcatcaat
     5941 cgctgctttt acctgatccg gggcggaaat accaaatccc tgcaatggag gtgcagcgtt
     6001 gtactctttc agcttcgcaa ccagatgatt gaggggtaac gcggcgcggt tttctgcgcc
     6061 ggtcacgcct gctcgtgaca gcaaataggt gtaaccacga ccgtaagagg ctatctggcg
     6121 cagcaggtcg tcatcggcat ttggcgggca gatgaagata ggtgcgacat tatgacgcaa
     6181 cgcggcctgg cggaagggcg cggactcttc aactggcaca tcggcaacca gcaccgaatc
     6241 gacgccgact ttttcgcact gggcataaaa ctcatcaatg cctttgttaa acaccagatt
     6301 ggcatacatc aacaggccaa tgggaatggt cgggtgtttc tggcgaatca gtgccagcat
     6361 ttcaaaacat tgtgccggag tcacacctgc cgcaaaggcg cgcagagtgg cgttttgaat
     6421 cgtcgggcca tccgccagtg ggtcggagaa ggggatacct aactccagcg cgtcagcacc
     6481 ggcttcaatt agcgtatcga taattttcaa tgactgctca atgcccggat caccgagcgt
     6541 gacgaaagga acgaatgcgc cttctttgcg ctccttcaac tgggcaaaca gagattcgta
     6601 gcgttccatc agatttcccc tcgtgctttc aaaatatcgt gaacggtgaa gatgtcttta
     6661 tcgccgcgac cggaaaggtt aaccaccagt agctgctctt tatccgggtt ttcgcgcatc
     6721 attttcaacg catgggccag ggcgtgggag gattccagcg ccgggatgat cccttcgtgc
     6781 aggcacagcg ttttgaaggc ttcaagggct tcatcatcgg taatagacac gtaatcagcg
     6841 cgtccagtgc tgttaagata cgcgtgttgt gggccgacag acgggaaatc cagtccggcg
     6901 gagatggagt aagattcttc aatctgcccg tcttcggttt gcatcatcgg cgctttcata
     6961 ccgaaataga tacccacgcg accatgtttt agcggtgcgc cgtgctcgcc agtttcgata
     7021 ccgtgaccac ctggctccac accaatcagg ccgacgttgg tttcattgat gaaatcagca
     7081 aacatgccga tggcattcga accgccgcca acacaggcga taacggcatc cggcaggcga
     7141 ccttctcttt ccagaatctg cgctttggtt tcttcgccaa tcatccgctg aaactcacgc
     7201 acaatggtcg gataaggatg cgggccagct gcggtgccca gcatatagtg cgcggtttcg
     7261 taactaccgg accagtcgcg cagcgcctcg ttacaggcat ctttcagcgt cgcggaaccg
     7321 ctatgcaccg ggatcacttc cgcacccatt aagcgcatac gaaaaacgtt aggcgactgg
     7381 cgttcaacgt ctttggcacc catataaata cggcatttca ggccgagcag ggcgctggca
     7441 agggccgacg ccacgccatg ctgaccggca ccggtttcgg cgatgatttc ggttttaccc
     7501 atccgcttcg ccagcaacgc ctgccccagc acctggttag ttttatgcgc gccgccgtgc
     7561 agcaaatctt cacgcttgag atacagcgta gtgttcgtcc cggctgtaat gttctggcat
     7621 ttggtcagcg cggttggacg cccggcatag tttttcagca ggtcgttgaa ctgagcctga
     7681 aattcaggat ctttttgcgc actgacaaaa gcttcttcca gctggcgcag agcaggcatc
     7741 aggatttgtg gcacgtacat gccgccaaac tcaccaaaat aggggttaag taatgttgtc
     7801 attgttcctt tccttaatat gcgcgcagcg tctggaaaac cgaggccaaa agacgtgcgt
     7861 ctttgatgcc cggttgcgac tctacagcag aattaaaatc aagtccggcg cagccggttt
     7921 gtgccgcttc cacgcagtta tctgcgccta agccccccgc cagcagaacg ttgccaagcg
     7981 attgaccatt taatagtgac cagtcaaaac gttgcccgct tccaccctgg ccgttgtcta
     8041 aaacatattt atcaacgtgc tgaaactcgc gggcgggcag ggtttcaccg acgcttaatg
     8101 ctttccagat ggcaacatgt gctggcagag cttcacgcag cgtatcgata tacagctgtt
     8161 cttcattacc atgcagttgc actgccgcca gcgataacac cttagctttg tccaccacat
     8221 cggcaatatc gtgattgcgg aacacgccaa catactgcaa cggtgccgca gccatcactt
     8281 cctgcgcctg ttcaacgttg acgcaacgcg gtgatgtcgc aacaaaaatc aacccaccgt
     8341 aaatcgcgcc cgcgtcataa gctgctttag catcttgccc acgcgtcagg ccacatactt
     8401 tattctcacc cagcaacacc cggcgcacgg cggcgtgcaa atcgtcatgg gccatcaacg
     8461 ccgaaccaat cagaaaaccg ttagcgaagt ggcttaactc gcgcacctga gcgtaagtat
     8521 tgatgccgga ttcgctgatt accgtcacgt tgtgccccag tttcggcgca agctcgcggg
     8581 tacggttgag atcaatcgac aaatcacgca gatcgcggtt gttgatgcca acgacctttg
     8641 cccccaatgc aatggcacgc tccagttcct cttcattact gacttcggtc agcacaccca
     8701 tctccagact gtgggcgacg gcggcaagct ggcgatattg ttcgtcatcc agtactgaaa
     8761 gcattaataa gcaggcatca gcctggtaat agcgcgccag atagatctgg taaggatcga
     8821 taatgaagtc tttacataaa atcggctgcg gggcgatttg gctgacgatg gggaggaaat
     8881 caaagctccc ctgaaaatat ttctcatcag tcagcaccga aattgccgaa gcgtaatgtt
     8941 tataaatggc ggcaatgcgt gctggatcga aatcatcacg gatcacgcct tttgacggcg
     9001 acgatttctt gcactccaga ataaacgccg tgcgcgcacc ctgtagcgca tcataaaaat
     9061 gtcgcgtgct cggctgaacc tcattctgaa aactggccag cggttgctgc tgtttgcggg
     9121 tttctaccca aatcgccttg tctgcgacga ttttcgctaa aacggtttgc atcatttacc
     9181 ctcgtgccgc cagtgcggta actctgtcgt aagcggaacc actgcgcagt acctcaagaa
     9241 cggtttgcgc attggcttgc agatcttcat ggccatgcag gcgcattaac atggcgacgt
     9301 tcgcagcgac ggctgcttca tgggcggcgt cgcctttacc ttgtaacaag cgtgttaaaa
     9361 tgtcacggtt ttcttccggt gttccgcctg ccagttgctc ctggtggtag ggagtcaggc
     9421 caaaatcttc agcggtcaat tgatagctct taatttcgcc gtcatgcagc tcggcaacga
     9481 ttgtcggcgc gtgtaatgaa acttcatcca tcccgccgct gtgcaccacc gccgcgcgtt
     9541 gataccccag cacgcgcaag gtttcggcaa tcggcagcac cagttccgga ctataaacac
     9601 caattaacgc cagcggcgga tgcgccgggt taatcaatgg ccccagcaca ttgaacaggg
     9661 tgcgggtttt cagttgctgg cgaaccggca tcgcgtggcg gaatccggtg tgatacttcg
     9721 gcgcaaagag gaaacataca cctaactcat ccagcgcctg gcgcgattta tcggcgttca
     9781 tatcaagatt aataccgaac gccgccagca gatcggacga accagattta ctggagacgc
     9841 tacggttgcc gtgtttcgcc actttcagcc cacaggccgc ggcgacaaac gcactggcgg
     9901 tagaaatatt gatactgttg ctgccgtcac cgccagtacc gacgatatca gcaaacagat
     9961 aatccgggcg cgggaacggc gcggcgtttt ccagtagcgc ggttgctgct ccggcgatct
    10021 cgttcgggtg ctcaccgcga attttcatgc tcaccagcgc cgccgccagt tgttccggct
    10081 tcagctcgcc acgcaccacc gctgaaaaca gctggtggct ttcttgttgg ctaagcgtct
    10141 gcgcctgata cagtttttcc agaatcggtt gcagcgtgtt ggctggctct agtttctgct
    10201 gcgcccaggc cagcgtttgt tccagcaggc gagcgccctg ggtggtgaga atggattccg
    10261 gatggaactg gaatccacaa acgcgatccg catcgtgacg tactgccatc accatgccat
    10321 taaaatgggc gttgatggtt aaaccggccg gaatgttact gccaaccagc gagtgataac
    10381 gcgccaccgg cagcgggttt gttaatccgg caaacatcgc ctgaccgtca tgttcaatgc
    10441 tcgacgcttt accgtgaaga atttcgcccg cctgaccgac atagcccccg taagcttcga
    10501 caattgcctg atgtccgagg caaatgccaa taatgggcag cttgccacgc aagcgggtga
    10561 ggagttccgg catacaaccg gcttcgctcg gcacaccggg gccaggagaa agcatcagta
    10621 ccggattgct catcgtcgcc aggcgttcaa ttaaggtctg cgccggaata tggttgcggt
    10681 aaatcaccac attatgccca ttgctgcgca actgatctgc caggttgtac gtaaaagagt
    10741 cgatattatc gagcagcaga atgtcagcca tcagaaagtc tcctgtgcat gatgcgcggt
    10801 ggcaatagcg cgcagtacag cgcgggcttt attacgggtt tcgtcggctt ccgactgcgg
    10861 aacagaatca aggactacgc cagcaccggc ttgcacggtg gcgataccgt tttccaccag
    10921 cgccgagcgg atcacaatgc aggtgtcgag atcgccatgc gcggtgaaat aacctaccgc
    10981 gccgccgtag ctgccgcggc gacgaccttc cgcctcggca attaactgca tagcgcgtac
    11041 tttcggcgca ccacttaacg ttcccatatt catacaggcg cggtaagcgt gcagggcgtc
    11101 gagatcgtgg cgcagctcac cgaccacgcg ggagaccaag tgcatcacgt aagagtaacg
    11161 gtcaactttg gtgaggtcgg cgacgtagcg gctgccgggg gtgcaaatgc gtgccagatc
    11221 attacgggcg agatccacca gcatcagatg ttcagaaagc tctttatgat cggtacgcat
    11281 ttccagttcg atgcggctgt cgaggtctct gtccagtgaa ccatcggcac gacgaccgcg
    11341 cggtcgtgtt ccggcaatcg ggtagatctc aatctggcgg ctggtggcgt catacttgag
    11401 cgaactttcc ggcgacgcgc caaacagggt gaaatcatta tcctgcataa aaaacatgta
    11461 cgggctggga ttactctttt tcagcacgta ataggccgcc agcggtgacg ggcagggcag
    11521 agagaaacgg cgagatggca ccacctggaa aatttctccg gcgcgaatcg ctttttgcaa
    11581 caaacgcact acgccaccga actcttcatc gctctggtta cattcacaac gcatatgcgg
    11641 cacggaaacc accggcagcg gtggcgcggt ttcggtcagt tgctggcgaa gatcgttcag
    11701 gcgagcagtg agacgttgtt tttcttcttc attcggagca aacaggctgg cctgaatgcg
    11761 ggtgcttttt ttctgatggt caatcaccat cagcgtttca gcgagataaa aacagaaatc
    11821 aggacagtta ttttccgctg acaattgcgg taaatcttca aaccccgcca caaggtcata
    11881 agagaacagg ccgccgaaga acattgcttc tcgttcttcc ttcggtacat tcagcatatt
    11941 ctgcaataag cggaaagcgt caaaaaccga aagggagcat aagcgagcgt cttcatccag
    12001 cagtggactg acagggggga agcgcagcac acggcagttt ggtaattgtt cattttccat
    12061 acccgcaggc agggcgttat ccagtagtgt cagcagggct tcgccgttgc cggaaagtgc
    12121 ctggattgtg acagtgtcac ctaaagctgt aatgcgcagc gcactgtcta ccagcagcag
    12181 gctttttaaa tcatctttgc tgtcgatatc tgcggattcc agcagcagcg ttgccggacg
    12241 atccccacac aactggtgaa aaagcgcggt gggattgtcg cgataagcgc cttcgcaggt
    12301 tagcagttcg agagtcggtt tttgtgtttg cattgttatt ctctaatttt gttcaaaaaa
    12361 aagcccgctc attaggcggg ctgggtatct gattgcttta cgcatggtga atacactgcc
    12421 cgtttcagga agtgcgccac caacctttca gtacgaaaat tgctttcatt gtcgataccc
    12481 tttttacgtg aacttgcgta ctagttaact agttcgatga ttaattgtca atagctcatt
    12541 tcagaatatt tgccagaacc gttatgatgt cggcgcaaaa aacattatcc agaacgggag
    12601 tgcgccttga gcgacacgaa ttatgcagtg atttacgacc tgcacagcca taccacagct
    12661 tccgatggct gcctgacgcc agaagcattg gtgcaccgtg cagtcgagat gcgcgtcggc
    12721 accctggcga tcaccgacca tgacaccaca gctgcaatcg cgccagcaag agaggaaatt
    12781 tcacgttcgg gactggcgtt gaatcttatt cccggcgtgg aaatttccac ggtctgggaa
    12841 aatcatgaaa ttcatattgt cgggctgaat attgatatta ctcatccgct aatgtgtgag
    12901 ttcctcgcgc agcagacaga acggcgcaat cagcgggcgc agctgattgc cgaaagactt
    12961 gaaaaagcgc aaatacctgg ggcgctggaa ggcgcacaac gactggcgca gggcggcgca
    13021 gtgacgcgcg gtcattttgc gcgtttcctt gttgagtgcg gcaaagccag ttcaatggcg
    13081 gatgtcttta aaaagtatct ggcgcgcggg aaaaccggat acgttccgcc acagtggtgt
    13141 acaatagaac aagctattga tgtcattcat cattctggcg gtaaggcggt gctagctcat
    13201 cctgggcggt acaatcttag tgctaaatgg ctgaaaagat tggttgcgca ttttgccgaa
    13261 caccacggtg acgcgatgga agtcgcgcag tgccagcaat cgcccaatga acgtacccag
    13321 ctggcggccc ttgcgcgtca gcatcattta tgggcatcac aaggatctga ttttcatcag
    13381 ccatgcccgt ggatcgaact gggtcgtaaa ctctggctgc ccgcaggcgt tgaaggcgtc
    13441 tggcagctat gggaacagcc gcagaacacc acagagaggg aattatgagc cagttttttt
    13501 atattcatcc tgataaccca cagcaacgtc tgatcaacca ggcggtggag atcgtgcgta
    13561 aaggcggggt gattgtttat ccaactgatt ccggctatgc gctcggctgt aaaattgaag
    13621 ataaaaacgc gatggagcgt atttgtcgta ttcgccagct gccggacggt cacaacttta
    13681 ccctgatgtg tcgcgatctt tctgaactct cgacctattc atttgttgat aacgttgcgt
    13741 ttcgtttaat gaaaaacaac acgccgggca actatacctt catcttgaag gggacgaagg
    13801 aagtgccacg ccgtctgttg caggaaaaac gcaaaaccat cggtatgcgt gtgccttcta
    13861 acccgattgc gcaggcgttg cttgaagcac tgggcgaacc gatgctttcc acttcgctaa
    13921 tgctgccagg cagcgaattt accgaatccg atcccgaaga aattaaagac cgtctggaaa
    13981 aacaggtaga tttgattatt cacggcggct atctcgggca gaaaccgaca acggttattg
    14041 atcttaccga tgataagccg gtcgtggtgc gtgaaggcgt aggtgatgtg aagcctttct
    14101 tataagtgcg taactctgta tactacgcgg ccttaaaatg gcgctatacg gcgccattga
    14161 ttctcctgtt cgacgcctgt gaaggcgaca ctaaaaggaa gctctatgag cgaaaaatta
    14221 cagaaagtgc tggcccgcgc tggccacggc tcccgccgtg aaatcgaatc tattattgaa
    14281 gccggtcgtg tgagtgttga tggcaaaatt gctaaactcg gcgatcgcgt tgaagttacc
    14341 cctgggctga aaattcgtat cgatggtcat ctgatttcgg tacgtgagtc cgctgaacaa
    14401 atttgtcgcg ttctggccta ttacaaaccg gaaggtgagt tgtgtacccg taacgatccg
    14461 gaagggcgtc cgacggtgtt tgaccgtctg ccaaagctgc gtggcgcacg ctggattgcc
    14521 gtaggtcgtc tggacgttaa cacctgtggt ctgctgctgt tcaccaccga tggtgaactg
    14581 gcaaaccgtt taatgcatcc aagccgtgaa gttgaacgtg aatacgccgt gcgtgtattt
    14641 ggtcaggttg acgacgcgaa actgcgtgat ttgagtcgtg gcgtgcaact ggaagatggt
    14701 ccggcagctt ttaaaaccat caagttcagc ggcggcgaag ggatcaacca gtggtacaac
    14761 gtgactctga ccgaaggccg taaccgtgaa gttcgtcgtc tgtgggaagc ggttggtgtg
    14821 caggtgagcc gcctgatccg tgttcgttac ggtgatatcc cactgccaaa aggtctgcca
    14881 cgcggtggct ggacggagct ggacctcgcc cagactaact atctgcgcga actggtggag
    14941 ctaccgccag aaaccagctc taaagtcgct gtagaaaaag accgtcgtcg catgaaggcg
    15001 aatcagattc gtcgtgcggt gaaacgtcac agtcaggtga gcggcggtcg tcgttctggc
    15061 ggacgtaata acggttaatc aaagtatgcc ggacgtcata tccggcattt ttacagatta
    15121 gtaatcaatc cctatctgcg cttttacacc ggcatcaaac gcatgtttga cggggcgtaa
    15181 ttcacttacc gtatctgcca gttcaagaat atcccgatga caaccacgac ccgtgataat
    15241 caccgtctgt tgatgtggac gttcatttaa cgcctgcacc acttcttcca gtggcaaata
    15301 gtcatacgcc accatatacg tcagttcatc aagcaaaacc atatccagtg aggaatcagc
    15361 aagcatccgc tttgcatgtt gccagacttc gcggcaggcg gcggtatcag actcgcggtt
    15421 ttgtgtatcc caggtaaagc ccgttgccat cacctgaaac tcaacgccat gtggctccag
    15481 cagattgcgt tcgccattag gccaggtgcc tttaataaac tgcacgacac ctactttttt
    15541 tccgtgacca actgcgcgtg ttgccgtacc aaatgccgcg gtggttttgc cttttccatt
    15601 gccggtaaag acgatgataa taccgcgttc atcctgggcc tgggccacac gagcatctac
    15661 tttttctttc actcgctgct gacgctgttg gtagcgttca tcactcattg ggaaattcct
    15721 ggtttacggc ccggttgggc gtcaaaggtc atgccggttt tacggcggct gtcatcgccc
    15781 atcagccaga ggtagagcgg catgatatca gcgggtgttt taagtttctg tggatcttcg
    15841 gtcgggaagg cgctggcacg cattgcggtg cgcgtaccgc ctgggttaat gcagttgacg
    15901 cgcaggcgct gctgatattc atcggccagt acctgcatca tcccttcggt ggcaaatttc
    15961 gacgctgcat atgcacccca gttagctcgt ccctgacgtc caacgcttga tgaagtaaag
    16021 accagtgaac cggcgtccga tttgagtaat aaaggaagca gcgcctgggt gagcaaaaag
    16081 gtggcattaa cgttgacctg catgacgtcc tgccagacct gcggattttg ttcgctcatt
    16141 gggcaaacat cgccgagcaa tccggcatta tgcaaaacac catccagacg cggataatta
    16201 acggtaatgc gctgtgccag ttgttggcaa tcttcggacg tgcaggtcag caaatcgaga
    16261 ataaaccact gtggctgacg tccagtttct tcgtttatgt ggctggctac ctgacgtaat
    16321 ttttcttcat tacggcccaa cagaatcact gtcgcaccat agcgtgcata cgtcatcgcg
    16381 gcttcacggc caatcccatc gctggctccc gtcaccagga taatgcgatc attgagtaaa
    16441 tcttgttttg gctggtaatg catggctact cctcaacgac gttgtctgtc gcgctgccag
    16501 tgtactttat gactttgggg ctttatgcct gaaacagaag ggcatttcaa tcagcctggg
    16561 gcgataacac cgcaaaatgg atactttgtc atactttcgc tgcaataaca tctctgcgag
    16621 acggcttaac atgcctgttg taaactgtga gccaaagcgt tgtttaacca aggtggggac
    16681 tcgtggaatt gttgtctgaa tatggtttgt ttttggcgaa aatcgttacc gttgtgctag
    16741 cgattgcggc gattgccgcc attattgtca atgttgctca acgtaataaa cgccagcgtg
    16801 gcgagttacg ggtcaacaat ctcagcgaac agtataagga gatgaaagaa gaactggccg
    16861 cggcgctgat ggactcacat cagcaaaaac agtggcacaa agcgcagaag aaaaagcaca
    16921 agcaagaagc gaaagcagca aaagcgaaag ccaaactggg cgaggtggca actgacagta
    16981 aacctcgcgt ctgggtgctg gattttaaag gcagcatgga cgcccatgaa gtgaactcgc
    17041 tacgtgaaga gataacggcg gtactcgcag cattcaaacc gcaggatcag gttgtgctcc
    17101 gtctggaaag ccctggtggc atggtgcatg gttacgggtt ggcggcttcg cagctgcagc
    17161 gtctgcgtga taaaaacatt cctttaactg ttacggtaga caaagtcgct gccagcggcg
    17221 gttacatgat ggcctgtgtg gcggacaaaa ttgtttccgc accgtttgct attgtgggtt
    17281 ccattggggt ggtggcgcaa atgcccaact ttaaccgctt cctgaaaagc aaagatattg
    17341 atatcgaact gcacactgcc gggcagtata agcgtactct gaccttgctg ggtgaaaata
    17401 ccgaagaagg gcgggagaaa ttccgcgaag agttgaacga aacgcatcag ttgtttaaag
    17461 attttgtgaa gcgtatgcgt ccgtctctgg atattgaaca ggtggcaacg ggtgaacact
    17521 ggtacggaca acaggcggta gagaaaggcc tggttgatga aatcaacacc agtgatgaag
    17581 ttattcttag cctgatggaa ggccgtgaag tggttaatgt acgctatatg cagcgtaaac
    17641 gactcattga ccgattcacc ggcagcgcgg cagagagcgc cgatcgattg ttgctacgct
    17701 ggtggcagcg gggtcaaaag ccattgatgt aaaagacaaa cgcgaggcta agacctcgcg
    17761 ttttgcttta atcaaccaga tgatattttt ctgaaagcac atgggccagg tgtttgaaca
    17821 tattaaacac cgcggtgctt ttggctgttg gcaatccttg ttcatctaaa aagtagtcgc
    17881 cggtaaatac cagcacgcca ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg
    17941 tatcctcatg ctcacgaatg attttgttgg cctctttcaa cagcgtttcg cggtcgatgg
    18001 gttgtgtctc tttgttcatt atttactcct taaacaagga cattagtcta cgccaggcat
    18061 ggcttgcaga caaatatacc acgctggtgg taagagcgcc ttactggtaa ctttggattt
    18121 tgcatgctaa taaagttgcg tatcggattt tatcaggtac agtgtgacgc tttcgtcaat
    18181 ctggcaatag atttgcttga cattcgacca aaattccgtc gtgctatagc gcctgtaggc
    18241 caagacctgt taactcagtc acctgaattt tcgtgaacag agtcacgaca aggggttgat
    18301 atccgcagag aacgagtcca tatcggtaac tcgttgccag tggaaggttt atcaacgtgc
    18361 gacgcattcc tggaagaatc aaattaggta aggtgaatat gggtaaagct cttgtcatcg
    18421 ttgagtcccc ggcaaaagcc aaaacgatca acaagtatct gggtagtgac tacgtggtga
    18481 aatccagcgt cggtcacatc cgcgatttgc cgaccagtgg ctcagctgcc aaaaagagtg
    18541 ccgactctac ctccaccaag acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg
    18601 tcaaccgtat gggggttgac ccgtggcaca attgggaggc gcactatgaa gtgttgcctg
    18661 gtaaagagaa ggtcgtctct gaactgaaac aactggctga aaaagccgac cacatctatc
    18721 tcgcaaccga ccttgaccgc gaaggggaag ccattgcatg gcacctgcgg gaagtgattg
    18781 ggggtgatga tgcgcgctat agccgagtgg tgtttaacga aattactaaa aacgcgatcc
    18841 gccaggcatt taataaaccg ggtgagctga atattgatcg tgttaatgcc cagcaggcgc
    18901 gtcgctttat ggaccgcgtg gtggggtata tggtttcgcc gctgctatgg aaaaagatcg
    18961 ctcgtggtct gtctgccggt cgtgtgcagt cggtggcggt tcgcctggtg gtcgagcgtg
    19021 agcgtgaaat taaagcgttc gtgccggaag agttctggga agtcgatgcc agcacgacca
    19081 cgccatctgg tgaagcgttg gcgttacagg tgactcatca gaacgacaaa ccgttccgtc
    19141 cggtcaacaa agaacaaact caggctgcgg taagtctgct ggaaaaagcg cgctacagcg
    19201 tgctggaacg tgaagacaaa ccgacaacca gtaaacctgg cgctcctttt attacctcta
    19261 cgctgcaaca agctgccagc acccgtcttg gatttggcgt gaaaaaaacc atgatgatgg
    19321 cgcagcgttt gtatgaagca ggctatatca cttacatgcg taccgactcc actaacctga
    19381 gtcaggacgc ggtaaatatg gttcgcggtt atatcagcga taattttggt aagaaatatc
    19441 tgccggaaag tccgaatcag tacgccagca aagaaaactc acaggaagcg cacgaagcga
    19501 ttcgtccttc tgacgtcaat gtgatggcgg aatcgctgaa ggatatggaa gcagatgcgc
    19561 agaaactgta ccagttaatc tggcgtcagt tcgtagcctg ccagatgacc ccagcgaaat
    19621 atgactccac gacgctgacc gttggtgcgg gcgatttccg cctgaaagca cgcggtcgta
    19681 ttttgcgttt tgatggctgg acaaaagtga tgcctgcgtt gcgtaaaggc gatgaagatc
    19741 gcatcttacc agcagttaat aaaggcgatg ctctgacgct cgttgaactt acaccagccc
    19801 agcactttac caagccgcca gcccgtttca gtgaagcatc gctggttaaa gagctggaaa
    19861 aacgtggtat cggtcgtccg tctacttatg cgtcgatcat ttcgaccatt caggatcgtg
    19921 gctacgtgcg agtagaaaat cgtcgtttct atgcggaaaa aatgggcgaa atcgtcaccg
    19981 atcgccttga agaaaatttc cgcgagttaa tgaactacga ctttaccgcg cagatggaaa
    20041 acagcctcga ccaggtggca aatcacgaag cagagtggaa agctgtactg gatcacttct
    20101 tctcggattt cacccagcag ttagataaag ctgaaaaaga tccggaagag ggtggtatgc
    20161 gcccgaacca gatggttctg accagcattg actgcccgac ttgtggtcgc aaaatgggga
    20221 ttcgcacagc gagcaccggg gtattccttg gctgttctgg ctatgcgctg ccgccgaaag
    20281 agcgttgcaa aaccaccatt aacctggtgc cggaaaacga agtgctgaac gtgctggaag
    20341 gcgaagatgc tgaaaccaac gcgctgcgcg caaaacgtcg ttgcccgaaa tgcggcacgg
    20401 cgatggacag ctatctcatc gatccgaaac gtaagttgca tgtctgtggt aataacccaa
    20461 cctgcgacgg ttacgagatc gaagagggcg aattccgcat taaaggttat gacggcccga
    20521 tcgttgagtg tgaaaaatgt ggctctgaaa tgcacctgaa aatggggcgt ttcggtaaat
    20581 acatggcctg caccaacgaa gagtgtaaaa acacacgtaa gattttacgt aacggcgaag
    20641 tggcaccacc gaaagaagat ccggtgccat tacctgagct gccgtgcgaa aaatcagatg
    20701 cttatttcgt gctgcgtgac ggtgctgccg gtgtgttcct ggctgccaac actttcccga
    20761 aatcgcgtga aacgcgtgcg ccactggtgg aagagcttta tcgcttccgc gaccgtctgc
    20821 cggaaaaact gcgttatctg gccgatgcgc cacagcagga tccggaaggt aataagacca
    20881 tggttcgctt tagccgtaaa accaaacagc aatatgtctc ttcggaaaaa gacggaaagg
    20941 cgactggctg gtcagcattt tatgttgatg gcaaatgggt tgaaggaaaa aaataacctt
    21001 taattctgtc aggtttttat aaacaaaggg tcgcgaaagc ggcccttttt tattgcatat
    21061 tattttttct tcacacctat acactaaggc tataaatgat atagtggtta tagttagcac
    21121 cttttttatt attaaatcgt attagtcacc cgccaggtgt gacgaaaaaa cgatgttctg
    21181 atggcgtcta agtggatggt ttaacatgaa attacaacaa cttcgctata ttgttgaggt
    21241 ggtcaatcat aacctgaatg tctcatcaac agcggaagga ctttacacat cacaacccgg
    21301 gatcagtaaa caagtcagaa tgctggaaga cgagctaggc attcaaattt tttcccgaag
    21361 cggcaagcac ctgacgcagg taacgccagc aggacaagaa ataattcgta tcgctcgcga
    21421 agtcctgtcg aaagtcgatg ccataaaatc ggttgccggt gagcacacct ggccggataa
    21481 aggctcgctg tatatcgcca ccacgcatac ccaggcacgc tacgcgttac caaacgttat
    21541 caaaggcttt attgagcgtt atccccgcgt ttctttgcat atgcaccagg gctcgccgac
    21601 acaaattgct gatgccgtct ctaaaggcaa tgctgatttc gctatcgcca cagaagcgct
    21661 gcatctgtat gaagatttag tgatgttacc gtgctaccac tggaatcggg ctattgtagt
    21721 cactccggat cacccgctgg caggcaaaaa agccattacc attgaagaac tggcgcaata
    21781 tccgttggtg acatatacct tcggctttac cggacgttca gaactggata ctgcctttaa
    21841 ccgcgcaggg ttaacgccgc gtatcgtctt cacggcaacg gatgctgacg tcattaaaac
    21901 ttacgtccgg ttagggctgg gggtaggggt cattgccagc atggcggtgg atccggtcgc
    21961 cgatcccgac cttgtgcgtg ttgatgctca cgatatcttc agccacagta caaccaaaat
    22021 tggttttcgc cgtagtactt tcttgcgcag ttatatgtat gatttcattc agcgttttgc
    22081 accgcattta acgcgtgatg tcgttgatgc ggctgtcgca ttgcgctcta atgaagaaat
    22141 tgaggtcatg tttaaagata taaaactgcc ggaaaaataa tttcttgcgt tattttcggc
    22201 accttttatg tagcgaaggt gccggaatat attctctttt gttacttatt tctataccgt
    22261 gcacaatatt tttatccctc atatttatag ggtaaattac ctgtaaagta gtgtctaatt
    22321 acccgcctcg caaactccag ttttcaaata tctatttcag gtcaaaagat tgaattattc
    22381 actggagacg attcgtaaat tcgctggatt tttgactaaa gtttctttag gatttgtctc
    22441 atcgtatgat taattacacc aatcgttcaa cgtcttgctg ataagtgatg gcgattatat
    22501 gaggttagca atgccttctg gaaatcagga accccgccgc gatcctgaat taaaacgtaa
    22561 agcctggctg gcggtttttc ttggttctgc acttttctgg gtggttgtcg cactgctgat
    22621 ttggaaagtg tggggataac tatggtcggt caggagcaac tggagtcgtc accattatgc
    22681 cagcatagtg acaatgaaac agaaacgaaa cgggaatgtt ccgtcgttat tccagacgac
    22741 tggcaactaa catcgcagca gcaagccttt atagaactgt ttgctgaaga tgatcagccg
    22801 aaacaataat tatcatcatt cttattaccc atttttaatg aattaaaggg cttttaatac
    22861 accgcagcaa taacagcttg agttatctca acacaaaata ataaccgtta agggtgtagc
    22921 ctatgatcaa cacaaatatg aaatattggt cctggatggg cgcgttttct ctgtcgatgc
    22981 tcttctgggc cgaactcctc tggatcatta ctcactgatc cttgaccccg ctgcggcggg
    23041 gttgtcattt gctttgccac aaggtttctc ctcttttatc aatttgggtt gttatcaaat
    23101 cgttacgcga tgtttgtgtt atctttaata ttcaccctga agagaatcag ggcttcgcaa
    23161 ccctgtcatt aaggaggagc tatgtcgtca accctacgag aagccagtaa ggacacgttg
    23221 caggccaaag ataaaactta ccactactac agcctgccgc ttgctgctaa atcactgggc
    23281 gatatcaccc gtctacccaa gtcactcaaa gttttgctcg aaaacctgct gcgctggcag
    23341 gatggtaact cggttaccga agaggatatc cacgcgctgg caggatggct gaaaaatgcc
    23401 catgctgacc gtgaaattgc ctaccgcccg gcaagggtgc tgatgcagga ctttaccggc
    23461 gtacctgccg ttgttgatct ggcggcaatg cgcgaagcgg ttaaacgcct cggcggcgat
    23521 actgcaaagg ttaacccgct ctcaccggtc gacctggtca ttgaccactc ggtgaccgtc
    23581 gatcgttttg gtgatgatga ggcatttgaa gaaaacgtac gcctggaaat ggagcgcaac
    23641 cacgaacgtt atgtgttcct gaaatgggga aagcaagcgt tcagtcggtt tagcgtcgtg
    23701 ccgccaggca caggcatttg ccatcaggtt aacctcgaat atctcggcaa agcagtgtgg
    23761 agtgaattgc aggacggtga atggattgct tatccggata cactcgttgg tactgactcg
    23821 cacaccacta tgatcaacgg cc
//
LOCUS       DAZFKM010000073        23554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFKM010000073 DAZFKM010000000
VERSION     DAZFKM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..164
                     /gene="xisR"
                     /locus_tag="ACHKAY_002955"
     CDS             <1..164
                     /gene="xisR"
                     /locus_tag="ACHKAY_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5701605.1"
                     /translation="KKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQA
                     SARPRRHLT"
     gene            199..1479
                     /locus_tag="ACHKAY_002956"
     CDS             199..1479
                     /locus_tag="ACHKAY_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701606.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHKAY_002957"
     CDS             complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHKAY_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO5701607.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHKAY_002958"
     CDS             complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHKAY_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO5701608.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHKAY_002959"
     CDS             complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHKAY_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO5701609.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHKAY_002960"
     CDS             4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHKAY_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO5701610.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4955..5515
                     /gene="speG"
                     /locus_tag="ACHKAY_002961"
     CDS             4955..5515
                     /gene="speG"
                     /locus_tag="ACHKAY_002961"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO5701611.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHKAY_002962"
     CDS             complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHKAY_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO5701612.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHKAY_002963"
     CDS             6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHKAY_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO5701613.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHKAY_002964"
                     /pseudo
     CDS             6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHKAY_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHKAY_002965"
     CDS             9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHKAY_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO5701614.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHKAY_002966"
     CDS             11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHKAY_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO5701615.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHKAY_002967"
     CDS             12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHKAY_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO5701616.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHKAY_002968"
     CDS             13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHKAY_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO5701617.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14049..15341
                     /gene="clcB"
                     /locus_tag="ACHKAY_002969"
     CDS             14049..15341
                     /gene="clcB"
                     /locus_tag="ACHKAY_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO5701618.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHKAY_002970"
     CDS             complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHKAY_002970"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO5701619.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHKAY_002971"
                     /gene_synonym="dgsA"
     CDS             complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHKAY_002971"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO5701620.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17469..18362)
                     /gene="ynfL"
                     /locus_tag="ACHKAY_002972"
     CDS             complement(17469..18362)
                     /gene="ynfL"
                     /locus_tag="ACHKAY_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5701621.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18469..19722
                     /gene="ynfM"
                     /locus_tag="ACHKAY_002973"
     CDS             18469..19722
                     /gene="ynfM"
                     /locus_tag="ACHKAY_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5701622.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(19771..19890)
                     /locus_tag="ACHKAY_002974"
     CDS             complement(19771..19890)
                     /locus_tag="ACHKAY_002974"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO5701623.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20146..20454
                     /gene="asr"
                     /locus_tag="ACHKAY_002975"
     CDS             20146..20454
                     /gene="asr"
                     /locus_tag="ACHKAY_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO5701624.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            20547..20630
                     /locus_tag="ACHKAY_002976"
     CDS             20547..20630
                     /locus_tag="ACHKAY_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701625.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            20730..21551
                     /gene="ydgD"
                     /locus_tag="ACHKAY_002977"
     CDS             20730..21551
                     /gene="ydgD"
                     /locus_tag="ACHKAY_002977"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO5701626.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21590..21919)
                     /gene="mdtI"
                     /locus_tag="ACHKAY_002978"
     CDS             complement(21590..21919)
                     /gene="mdtI"
                     /locus_tag="ACHKAY_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO5701627.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(21906..22271)
                     /gene="mdtJ"
                     /locus_tag="ACHKAY_002979"
     CDS             complement(21906..22271)
                     /gene="mdtJ"
                     /locus_tag="ACHKAY_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO5701628.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22683..22826
                     /locus_tag="ACHKAY_002980"
                     /pseudo
     CDS             22683..22826
                     /locus_tag="ACHKAY_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
BASE COUNT         6112 a         5900 c         5647 g         5895 t
ORIGIN      
        1 taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg
       61 acggtatgcc atgggataac agtccatgct tttataacct ggaagaaatt gatcgctgga
      121 ttgagcgcca ggcatcagcg agaccaagac gtcatcttac ttgactaaaa gccacactaa
      181 ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta
      241 cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa atctgggggt tcctgacaca
      301 tcaaaaaaca ggcgcgttgc aggtgaacta cgctcctctg tttgttacgc aataaaaact
      361 ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa atttggtgag
      421 gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttttggcact gaaagaaact
      481 gaagtcgcca aaacatcact caacacatac cgtgccgtca tcaaaaatat cctgagcata
      541 atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga ggttcgtaaa
      601 gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca accagggaga
      661 tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg ccgtgttcca gtttggtgtt
      721 gataacggtt acctggcaga taatccgttt aaggggatct cgccattaaa ggaatcaaga
      781 accattccgg atcctctttc gcgggaggaa tttatccgtc ttatcgatgc gtgcagaaat
      841 cagcaagcaa aaaatttatg gtgtgtttct gtttatactg gcgttcgccc tggtgagctg
      901 tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat caggagaaat
      961 ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg ggtcattcat
     1021 cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag actgagcaaa
     1081 gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca aaaatgcacc
     1141 tttgtttttc aacctgaagt gtcagcgaga gtaaaaaatt atggtgacca ttttaccgtt
     1201 gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg ccatcgaaaa
     1261 tcatatcagt cgagacatac ttatgcctgc tggtcgctga cagctggtgc taacccggca
     1321 tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt atacggaaaa
     1381 tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt aagcaagttt
     1441 gccccaacca tgccccataa cgaagcaatg aaaaattaat ttaatattta tcaaatagtt
     1501 aacacgcatg actcttgaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     1561 ctgctttcac caaatcctta gtgcttcttt catgtttttc tattgtcata atggttatct
     1621 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     1681 tgagcaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg gcatcagcaa
     1741 catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac cctttactta
     1801 accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa atagagagtt
     1861 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     1921 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     1981 ccgcatcgat aattaatgtc ggcttgatgc ctttttcagc gaaaatctcg ccaagcggtg
     2041 tctggctgtt attaatcgcc cagtctgccc cgctctcttt cgctttttcc agtcgttcat
     2101 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     2161 tcgtcaggcc gattggaccg gcaccataaa ccagaacggt atcattttca gtcggttgac
     2221 catgaccggt aacgttagcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     2281 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     2341 caccgtcagc gtgcacacct aatacagcaa gtgtcgtaca aacgttcggc ttacctatag
     2401 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgc tcaccgactc
     2461 tggcgctttc cacaccttca cccaccgcat caatgacgcc aaagaattca tgaccaatga
     2521 cgcgcggata tttcgcaaaa ggattatgcc cacggtaaat atggctatct gaaccacaaa
     2581 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcacgtt
     2641 cgataatcga cagttgattc ggtttttcaa ttaaaatgct tttcattacc ttactcctta
     2701 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
     2761 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
     2821 catatagccg ttatcgaaag tccagttgtg cgggaagact tcgagcattt gttcggaata
     2881 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
     2941 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacagcga
     3001 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
     3061 gatataatcg atgagttgct cttcaatcag ttgtttgcag tcccagatgc tgttgaagac
     3121 ttcacccact gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
     3181 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
     3241 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
     3301 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
     3361 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
     3421 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
     3481 tttgaatccc agctcctgat gacgggcata atcatccaga gcttcatcaa tactgtgacc
     3541 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
     3601 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
     3661 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
     3721 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
     3781 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
     3841 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
     3901 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
     3961 gagctactac catacaagca taaagatcga aaaaagccgg agtgatcaca aaaaaaggcg
     4021 tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga ataccttttg tcagctgaca
     4081 cttcctctta tcttattgat aaaatggatt tatgttccta cgtgcgcccc cagcccgcaa
     4141 caatgatcaa catgccgcaa agcgcaatca acgcacccgt ccagtcataa agagtcagtt
     4201 tcacgccatc cacaacgcgc agccacatca acgccgtgca gacataaacg ccaccataag
     4261 ccgcgtaaac acgcccactc gccgctggat gcaacgttaa caaccagaca aacagcgcca
     4321 gtgaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
     4381 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
     4441 tcatctttgt ctcttattga catcatgtat agttataggg cgacataata tcatcaatat
     4501 aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt gtagaatcac ctttaatcat
     4561 tcatacaggg aatgaattat gaaaatcact ctcagcaaac gaatcggcct gctcgctatt
     4621 ctgctgcctt gcgcactggc attgagcaca actgttcatg ccgaaactaa caaactggtg
     4681 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
     4741 aatgacacgc gcaatctgcg ccagaaagtg aataaacgca ctgaaaaaga gtgggataaa
     4801 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
     4861 tgggagccca atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
     4921 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
     4981 acgcccgctg gagcgtgaag atttacgcta tgaacatcaa ctcgacaaca acgccagtgt
     5041 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     5101 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
     5161 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
     5221 tatctccccg gagtatcagg ggaaaggtct ggcaacccgt gccgccaaat tagcaatgga
     5281 ctatggcttt accgttctca atctctataa gctgtatctg atcgttgata aagagaatga
     5341 aaaagcgatt cacatttacc gcaagcttgg cttttcggtt gaaggtgaat tgatgcacga
     5401 gttctttatt aatggtcaat atcgtaatgc cattcgcatg tgtatattcc agcatcagta
     5461 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
     5521 ataataatcg atcgtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
     5581 atcaacaatg attagctgac agtccaccgg attagcgtga ctgtcatatt cacagctctg
     5641 ttttacatta ccaacccgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
     5701 gatcggatcc gttgatggcg tggcgctgac agataatgtt ttgtcgttac ttttcgtggt
     5761 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcattt gtttgctgct
     5821 ggctttaatc acgaagccat tatcatcggt ttcccaactc accccggcag aaggtaattc
     5881 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
     5941 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
     6001 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
     6061 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
     6121 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
     6181 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
     6241 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
     6301 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
     6361 cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc gccgggatcc tgtgagcgca
     6421 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacgt
     6481 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc ggccattgcg
     6541 ctaatgatac gcataaaatt ctctataccc gcaccaccag cggtaacgtc tctgctcccg
     6601 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
     6661 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
     6721 cttctattga tatggattaa taattcttaa cccaaaatgg gtagactccc tctattgtta
     6781 gcgcgctaaa tattcaatat ataaacattt atataacgat aaagaacagg gagtgagtta
     6841 tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa tcaaccgcga
     6901 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
     6961 cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc gtcaactgtg
     7021 gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg gtggaaactg
     7081 acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg cgcggtcgct
     7141 ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggta
     7201 cacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
     7261 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
     7321 cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg gtcaaacgcc
     7381 tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc gcgcaaattt
     7441 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
     7501 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
     7561 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg
     7621 atccgcgata taccgatacg gctgctggtc gtgaagatga atggctccct attcgcccgg
     7681 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
     7741 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
     7801 cacccaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat aacacagcga
     7861 aaacgccgca gtgggcttcg caaattaccg gtatcccggt agaccgtatc atcaaactgg
     7921 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
     7981 aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt acctattttg acgggcaatg
     8041 tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
     8101 gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacagatg
     8161 ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc aaagataaac
     8221 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt
     8281 ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtga
     8341 tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga
     8401 tgaccgttga gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca
     8461 ttttcctcca gcctgtcacc agcgaaaaat tcgaacgcaa accgatttac tggatcctga
     8521 gtgaagtcgc gaaacgtctt ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc
     8581 aggaacaatg gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt
     8641 cttacgacga actgaaaaaa tgggtatcta taagcgtaaa gatcccaatg gccattttgt
     8701 cgcctacaaa gcatttcgtg acgaccccga ggcaaatcca cttaaaacgc cttccggtaa
     8761 gattgaaatt tattccagca ggctggcgga aattgcccgt acctgggaac tggaaaaaga
     8821 tgaagtgata agcccattgc cagtttatgc ctcaaccttt gaaggctgga actcccctga
     8881 acgtagaacc ttcccactgc aactgtttgg tttccattac aaatcccgta ctcactcgac
     8941 ctacggcaat attgatctcc tgaaggctgc ctgccgtcag gaggtgtgga tcaaccctat
     9001 agatgcgcag aaacgtggga ttgccaacgg cgatatggtg cgggtgttta accatcgtgg
     9061 cgaagttcgg ctaccagcca aagtaacacc acgtattctc cctggagtta gcgctatggg
     9121 ccagggagcc tggcacgagg ccaatatgtc tggcgataaa atcgaccatg gcggctgtgt
     9181 gaatacgcta accactctgc gcccttcacc actggcgaag ggaaacccgc agcacactaa
     9241 tctggtcgag atcgaaaaaa tataacccac gacaaccata aattctggca tgacattttg
     9301 tttgaaaagc aataagtgag taatgatgaa aatccatacc acagaggcgc tcatgaaggc
     9361 tgaaatcagc cgtagaagtc tgatgaaaac ctccgcactt ggcagtcttg cgctggcaag
     9421 cagtgctttc actctgccat tttcccaaat ggtccgggcg gcagaggctc cggtagaaga
     9481 gaaagcggtc tggagttcct gcaccgttaa ctgcgggagc cgctgtctgt tacgtttgca
     9541 tgtgaaagat gacaccgtgt actgggtgga gtctgatacg acaggtgacg acgtctacgg
     9601 taaccatcag gttcgagcgt gtttacgcgg gcgctctatc cgccgacgga tgaatcatcc
     9661 tgataggttg aaatatccca tgaagcgcgt cggcaagcgc ggtgaaggta aatttgaacg
     9721 gataagttgg gacgaagccc tggataccat cagtgataat cttcggcgga tcctgaaaga
     9781 ttacggcaat gaggctgtac atgtcctgta cggaacaggc gtagatggcg gaaacatcac
     9841 caactcaaac gtcccgtacc gtctgatgaa ctcttgcggt ggttttctca gtcgctatgg
     9901 cagctacagt accgcacaga tcagtgccgc aatgagttat atgttcggtg ccaatgatgg
     9961 caacagcccg gatgatatcg ccaatacgaa actggtcgtt atgttcggaa ataacccggc
    10021 agaaacgcgg atgagcggcg gtggtgtcac ttactacgtc gagcaagccc gcgaacgttc
    10081 aaacgcacgc atgatcgtca tcgatccacg ttataacgac actgctgccg ggcgtgaaga
    10141 tgaatggctg cccattcgcc ctggcaccga tggcgcactg gcctgtgcga ttgcctgggt
    10201 actgattact gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga
    10261 tgaaaaaacg ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg
    10321 cgaagggcct gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc
    10381 ggcagaaaaa attatccagt tggcacgaga gatcggttca gcaaaacctg cttatatttg
    10441 tcagggttgg gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat
    10501 gctttccgtt ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg
    10561 tagctgggat ctgggggtag aatggttccc gatgcttgag aatcctgtta aaacgcagat
    10621 ttccgtcttt acatggacag atgccatcga ccatggtacg gaaatgaccg cgacccgtga
    10681 tggtgttcgt ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag
    10741 taacacattg atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga
    10801 cagcaagtgc gagatgattg ttggcattga ccacttcatg acggcctcgg ctaagtattg
    10861 cgatatcctg ttgcccgacc tgatgccaac agagcaagaa gaccttatct ctcatgaatc
    10921 tgcagggaat atgggctatg tgatcctcgc ccaacccgca acctcagcaa aatttgaacg
    10981 caaacccatc tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca
    11041 aacctttact gaaggtcgca gtcagcatga atgggtcaaa tatctccatg cgaaaacgaa
    11101 ggaacgtaac cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa
    11161 aaaatgcccg gaagaacact acgtcgcttt ccgcgcattc cgtgaagatc cacaggcaaa
    11221 cccgttgaaa acaccttcgg ggaaaatcga aatttattct gaacgactgg cgaagattgc
    11281 agatacctgg gaattgaaaa aagatgaaat tattcatccc cttcctgcgt ataccccagg
    11341 ttttgatggc tgggacgatc ccctgcggaa aacctatcca ctgcagttaa cgggcttcca
    11401 ttacaaagcg cgtacccact ccagctacgg caatattgat gtgttacagc aggcctgccc
    11461 acaagaggtg tggatcaacc ccattgatgc tcaggcacgc ggtatccgtc atggcgatac
    11521 cgtgcgggta tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat
    11581 tctgcctggc gttaccgcca tcgggcaagg tgcgtggctt aaagcggata tgtttggtga
    11641 ccgggtcgat cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc
    11701 aaagggaaac ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaag gagtagccca
    11761 tgaccacaca atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg
    11821 aactggcgtg caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg
    11881 aatacgctgg cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt
    11941 attatctctc catctcctgt aaccattgcg atgaccccgc atgtacaaaa gtctgcccca
    12001 gcggcgcaat gcataagcgt gaagatggct ttgtggtggt tgatgaagat gtttgtatcg
    12061 gctgccgcta ctgccacatg gcttgtccgt acggcgcacc acagtacaat gctgaaaaag
    12121 ggcacatgac gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat
    12181 gcgtcgaatc ctgcccgctg cgtgcacttg agttcggtcc aattgaagag ctgcgccaga
    12241 agcacggcac tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata
    12301 tcgttatcaa acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta
    12361 atccggagga agtgtaaaat gggaaatgga tggcatgaat ggccactggt tatctttacc
    12421 gtattgggtc aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc
    12481 aaaaatgacg cagaccgtca gcgtattgtc cgtggcatgt ttttcctctg gctgttgatg
    12541 ggcatcgggt ttattgcctc tgtcatgcac ctgggatcgc cactgcgggc gtttaactcg
    12601 ctgaatcgaa tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt
    12661 gccgttggcg gactatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg
    12721 aaactctggt tacttgtcag catggcgctc ggcgtcattt tcgtctggat gatgacctgc
    12781 gtgtatcaaa tcgacactgt gccaacctgg cataacggtt acactacgct ggctttcttc
    12841 ctgacggtat tgttgagcgg tccaatactt gctgccgcaa ttctgcgggc agcccgcgtt
    12901 acatttaata ccacaccatt tgccatcatt agcgttctgg cgctgatcgc ttgtgccggg
    12961 gtgattgtgc tgcaaggttt gtctctggcg tctattcact catccgtgca acaagccagc
    13021 gcactggtac cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt
    13081 cttggatgct ggctttgccc gctcattcgt cgccgtgaac ctcacgttgc cggacttatt
    13141 ctggggctaa tcctcatact tggcggtgag atgatcgggc gtgtgctctt ttatggcctg
    13201 cacatgacag taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat
    13261 ttcaggatgt taggaatgac ccatttttca cagcaagata atttttctgt cgcggcgcgc
    13321 gtgttgggcg cgctgtttta ttacgctcca gagagtgcgg aagccgcacc tctggttgcg
    13381 gtactcacca gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg
    13441 ctggtgacgg cttttcagac acagtgtgaa gagactcacg cccaggcctg gcagcgtttg
    13501 tttgtcggcc cgtgggcact gccgtctccg ccgtgggggt cggtctggct ggatcgcgaa
    13561 tctgtgctgt ttggcgattc aacattggca cttcgtcagt ggatgcgcga gaaaggcatt
    13621 cagtttgaaa tgaagcaaaa cgaaccggaa gatcattttg gttcactgct gttgatggct
    13681 gcgtggttgg cggagaatgg tcgccagaca gaatgcgaag aattgctggc atggcacctt
    13741 tttccgtggt caacacgttt tcttgatgtt tttatcgaaa aagcagaaca ccctttctac
    13801 cgtgcactgg gtgaactggc tcgcttaacg ctggcgcaat ggcaatcaca actgttaatt
    13861 cctgtcgcgg ttaaaccgct gtttcgatag gatcaccgta atattgccgg atggtgacga
    13921 ttacgcgcaa tccggcaata ataggttaca gtgtcacgtt tttttatctc ttaaagcacg
    13981 cactgctttt gcggctggcc tcttttgccg caaaatagtc gcccgtgttt cattgcccat
    14041 ttctgctcat gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc
    14101 ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcga
    14161 tgctgctact ggagtggttg ttcctcaata atgactccgg cagtctggtc aatgcagcga
    14221 caaacctttc cccctggcga cggttgctaa ctccggcgct cggcggactg gcggcgggtt
    14281 tgttgctgat gggctggcag aaatttaccc aacaacgccc tcatgcgccg accgattaca
    14341 tggaagcgtt gcaaaccgat ggacagttcg attacgcagc aagcctggtt aaatcgcttg
    14401 cctctctgct ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt
    14461 tagctgccct tgccgcctcc tgttttgccc aacgttttac gccacgccag gagtggaaat
    14521 tatggatcgc ctgtggggcc gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg
    14581 gcagtttatt tatagccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg
    14641 tgattatttc cgccgtcgtg gcattgctgg ttagcaatct gattaatcat agcgacgcgt
    14701 tactctacaa cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca
    14761 gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgtc
    14821 atcgtggatt tgtgagtctc aaacttgcgc cgccctggca actggcacta ggcgggttga
    14881 tcgtgggtct gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac
    14941 aatccttttt aaccgcccca ccactgttaa tgatcattgc cgggatcttc ctttgtaaac
    15001 tgtgtgccgt gctggcgagt agcggttccg gcgcacccgg tggtgtcttt acaccgacgc
    15061 tatttatcgg tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg
    15121 atggcgaaga aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa
    15181 ccacgcacgc gccgattatg tcgacgttga tgatatgtga aatgaccggg gagtatcagc
    15241 tactccccgg tttattgatt gcctgcgtca ttgcgtcggt aatttcgcgg acgttacacc
    15301 gtgactctat ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt
    15361 tctcgctgtt cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt
    15421 ttacccagca catcaatgat ttccgcataa tgcgccagtc ctgggttgat tcggttagcc
    15481 acccagccaa tgagcggcag cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg
    15541 ttaatgcaac cttcctgaat accgacaacc atcaacaccg gcaactgttc ctgcaccacc
    15601 cattcagaga gtggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc
    15661 acgacatgat cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg
    15721 attgggcaac tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct
    15781 tcataaggca gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc
    15841 ccttcgggtg tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc
    15901 tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct
    15961 gtaccggtaa taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat
    16021 aaaccaggaa aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga
    16081 tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa
    16141 agaaccgtta tacatcgcgt cttttaccag tgcagcgcct gccatcgtgc cctggttaga
    16201 aaactgagta ctctcaacgc tgatgtgctg actatacgca ggaagggcct gctgacggat
    16261 gctgtctgag atgaccggga agaggatatc tgccgcttta cttaacggtg agccaatcag
    16321 tattttttgt gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc
    16381 caccccggta atgatgtctt ttgccagcag atcgccgcgc aatgccgcct gacacaatga
    16441 gtccacggtt aacggttgtc catgtaacat cgagctcatg gattgattaa gacgcagctg
    16501 tgccagctca agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca
    16561 ataacagcgt ttcccatacg ggtcgacctg tgtgtggcct atttccacga gactactgct
    16621 gcctgcgtgt agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac
    16681 cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca
    16741 tgcgctgata tcatgctgaa tataaaccgg tacgccggta tgctgctcca gcgcctcgcc
    16801 gagcggcatc tcttttacat cctcgtagaa cggcatgcga tgtacaatac cattttccgt
    16861 atcaataatt cccggcaagg ttatggcaat cgaagttaga cgctcaagtt ttttctggtg
    16921 gcgaataaaa aactgatcga tatgggaaat gatacgatcc agcaatggcg agtcatcttt
    16981 taacgccagt tcctgcgact cttccaccac cagtttgctg ctcagatcgc gcagagcaag
    17041 gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac
    17101 cagccccacc gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag
    17161 gtgtgcttcg agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag
    17221 acgggaaaga tcgatacgcg agactggacc aagctgatca atcaggcgat aaaccgcgcc
    17281 cgcgttggtc tgctttattt gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata
    17341 ctccctatat tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt
    17401 gtcgtcaaat ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga
    17461 aatttccctc acctgagagc attcagcaga tgaatacgaa agttacgcgc agccggactt
    17521 tgttcatgat gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc
    17581 ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg aagcaggcaa aattgaaaca
    17641 cccagaccgg cggaaaccag accgatgatg gtcattgcct cgcccacctc ctgagtgatg
    17701 acgggcgtca aatggtaacg tcgcatcagc ccgagaatat cgtcatacag ccctgtcccg
    17761 acgtgcggat caaaaaagac gaagggttct ttcgccagtt cagccagcgt tacattcggg
    17821 ttatttgcca ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact
    17881 gcgtgttcaa gcgtctccgg taacgctgtg taacgcagca atcccatatc cagcgttcct
    17941 tcaatgagcg gagcgatttg ctcacgagtg ttcatttcgc gggtttgtaa atggacatcg
    18001 ggataatcac ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac
    18061 gaagtaaaac caatgcgcaa ctcccccgct tccccctgat gcagtctttc agcgcgagcg
    18121 gcagcgtcat ctaccataga caggatttgc cgactatctg caagaaactg ttttcctgct
    18181 gccgtcagca atacactgcg attagttcgt gccagcagtc gggcaccaat ttgttgctcc
    18241 agtgcctgaa tctgctgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc
    18301 ccgaaatgca gctcttccgc aacagcaaca aagtaacgca gatgacgaag ttcaatattc
    18361 atatttaaaa catcttattt gagattatta atatattaga cagaacaatt cgattttcct
    18421 aacctatgta taagcctgat ccacaggcat atttagcaag gatttcaagt gagccgtact
    18481 acaactgttg atggcgctcc ggcaagcgac actgacaagc aaagcatttc tcagccaaat
    18541 caatttatta aacgcggtac gccgcaattt atgcgcgtca ccctggcgct gttctctgcc
    18601 ggactggcaa catttgcact tctctattgt gtgcagccta tccttccggt gctttcgcag
    18661 gagtttggct taacgcccgc gaacagtagt atttcactgt ccatttccac ggcgatgttg
    18721 gctattggtt tgctgtttac tggcccccta tccgatgcca ttggtcgcaa accagtgatg
    18781 gtcacggcgc tactgttggc ctccatttgt acgttacttt cgacaatgat gaccagctgg
    18841 cacggcattt tgattatgcg cgccttgatt gggctttcgt taagtggcgt ggcagctgtt
    18901 ggcatgactt atcttagcga ggaaatccac cccagtttcg tggccttttc gatggggttg
    18961 tatatcagcg gcaactcaat tggcggcatg agcggacgct taattagtgg tgtcttcacg
    19021 gactttttca actggcgaat tgctctggcg gcaatcggtt gtttcgcgct agcctcggcg
    19081 ttgatgttct ggaaaatcct ccctgaatca cgccattttc gcccgacttc gctgcgacct
    19141 aaaacgttgt ttatcaactt tcgtctgcac tggcgtgacc ggggattacc gttattgttc
    19201 gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt ttaattacat cggatatcgg
    19261 ttgatgctct ccccctggca tgtcagtcag gccgtggttg gcttattatc gctggcttat
    19321 ttgaccggta catggagctc acccaaagcc ggaaccatga ccacccgcta cgggcgcggt
    19381 ccggtgatgt tgttttcgac gggggttatg ctgtttggtt tactgatgac cttattcagc
    19441 tcgctgtggc tgatctttgc cggaatgtta ctcttctcag caggattctt cgcagcccac
    19501 tcagttgcca gcagctggat tggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg
    19561 ctgtatctgt tcagttacta tctggggtcg agtattgccg ggacgctggg tggtgttttc
    19621 tggcataact atggctggaa cggcgtcggc gcatttattg ctctgatgct ggtcattgct
    19681 ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag cccggactgc
    19741 ggtaactaca cgtccggact tattgcttgc tcaaaccaac gttaatagcc atcctaaaat
    19801 agacgaagcg ccagccaatt cccgcagcgc gtctagcgtc atcaggatta taagtaccca
    19861 aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct
    19921 ttgtaagtag taaaatagtt aacccgatca agactactat tattggtagc taaatttccc
    19981 ttaagtcaca atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta
    20041 cacgctgaaa ccaaccactc acggaagtct gccattccca gggatatagt tatttcaacg
    20101 gccccgcagt ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta
    20161 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
    20221 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
    20281 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
    20341 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
    20401 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
    20461 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc aggtaacgtt
    20521 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
    20581 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
    20641 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
    20701 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
    20761 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
    20821 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
    20881 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
    20941 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acgccgccta
    21001 agggtaaagc ggataaggcg gtagcgctgc gttttgtgtc aaataaaggt ctttggcgct
    21061 atgacatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
    21121 atggggatgg ttggattgtt cctcccgcag ctgcaccatg ggacttcggc ttgattgtgc
    21181 tgcgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
    21241 ttactgccgc attaaaatcg gctggtcgta aagtgactca ggcgggatat cctgaagatc
    21301 atctcgatac gttatacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
    21361 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctttg atgttgcata
    21421 ccgatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
    21481 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
    21541 cgcaaaaata atgttcaaac gctgcccgac agtgcgggca gcgtgttcat caggcaagtt
    21601 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
    21661 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
    21721 atgcataagc gacagacaag tcgatccctt ttacggcctg agaaagcgca ctaaaggcag
    21781 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
    21841 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
    21901 cccattcaaa ctgcgccatg gttcacttcc agttcaggtt tacgcgcttt acgggtacct
    21961 gatttgatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
    22021 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
    22081 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
    22141 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
    22201 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
    22261 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
    22321 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
    22381 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
    22441 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
    22501 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
    22561 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
    22621 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
    22681 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
    22741 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
    22801 cattatttat tgctgttata tataacttat ttcgcataac gcattgattt aattaaataa
    22861 ttaacaccgc aaaaagtgcc aaaaatcgca aaatgactac accagtgact acaccgttcg
    22921 gtgcactgta tgaaacaacg tggaacaaat agacacaaga aatatacagg cggatcatct
    22981 ttccaggggg aagcacacca attcatgagg ggcgttaatg tcgatatggg gatccccacc
    23041 taccggaatt tttttccggt taaacgttaa tcaggctggt gggctttacc tgttcggtag
    23101 gtgttatgat tatcgtaata cctttccccc aatggggtaa aagcatcaac cgtaacgaaa
    23161 gtcgttatgg ttgccactga taccccaaat ttgggtatca cgagtaagcc ccaaaatctg
    23221 ggtgttactc ttactaccca aactacgggt agtttccgta gtttcatggt cgagttgcag
    23281 atctgcaact cccccattaa ccacaggcga atttccgcct tcggtcattc cttaatcatc
    23341 tgcgtaatca taatgattat tcagcaagcg caattttgcg ctttgcttaa agttcagcga
    23401 gttaccgcgc cgacttcctc aaattgaggt ttccgaaaat atcagcgggt tagcgattaa
    23461 gctgaccatt aattaaactg cgaaaatgtc gtagttgtga ggggtgtagc caacccattt
    23521 ttgggccgtc gggaatatca accagttacc gccg
//
LOCUS       DAZFKM010000074        23194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFKM010000074 DAZFKM010000000
VERSION     DAZFKM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            115..972
                     /gene="focA"
                     /locus_tag="ACHKAY_002981"
     CDS             115..972
                     /gene="focA"
                     /locus_tag="ACHKAY_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO5701629.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1027..3309
                     /gene="pflB"
                     /locus_tag="ACHKAY_002982"
     CDS             1027..3309
                     /gene="pflB"
                     /locus_tag="ACHKAY_002982"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO5701630.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3501..4241
                     /gene="pflA"
                     /locus_tag="ACHKAY_002983"
     CDS             3501..4241
                     /gene="pflA"
                     /locus_tag="ACHKAY_002983"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO5701631.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4323..4913)
                     /gene="ycaK"
                     /locus_tag="ACHKAY_002984"
     CDS             complement(4323..4913)
                     /gene="ycaK"
                     /locus_tag="ACHKAY_002984"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO5701632.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5013..5921
                     /gene="ycaN"
                     /locus_tag="ACHKAY_002985"
     CDS             5013..5921
                     /gene="ycaN"
                     /locus_tag="ACHKAY_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5701633.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5922..7352)
                     /gene="ycaM"
                     /locus_tag="ACHKAY_002986"
     CDS             complement(5922..7352)
                     /gene="ycaM"
                     /locus_tag="ACHKAY_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO5701634.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7561..8709)
                     /gene="ycaD"
                     /locus_tag="ACHKAY_002987"
     CDS             complement(7561..8709)
                     /gene="ycaD"
                     /locus_tag="ACHKAY_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5701635.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9024..9650
                     /gene="ycaC"
                     /locus_tag="ACHKAY_002988"
     CDS             9024..9650
                     /gene="ycaC"
                     /locus_tag="ACHKAY_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO5701636.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9686..10549)
                     /gene="dmsC"
                     /locus_tag="ACHKAY_002989"
     CDS             complement(9686..10549)
                     /gene="dmsC"
                     /locus_tag="ACHKAY_002989"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO5701637.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10551..11168)
                     /gene="dmsB"
                     /locus_tag="ACHKAY_002990"
     CDS             complement(10551..11168)
                     /gene="dmsB"
                     /locus_tag="ACHKAY_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO5701638.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11179..13623)
                     /gene="dmsA"
                     /locus_tag="ACHKAY_002991"
     CDS             complement(11179..13623)
                     /gene="dmsA"
                     /locus_tag="ACHKAY_002991"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO5701639.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13923..14915)
                     /locus_tag="ACHKAY_002992"
     CDS             complement(13923..14915)
                     /locus_tag="ACHKAY_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701640.1"
                     /translation="MSIKQLKDGRYQVDVRPQGAEGKRVRKIFALKSKAQEFEKYVLQ
                     NFHDKPWQAKPADQRRLSELLDAWWMLDGRNQAYGDSYRVRLGKVVREMGDPRASQMT
                     RKFMLEYRSEKLQAGLMPSSINRDLCVLSTMFTVLIEAEVFHNANPVRGIRKLKVQNT
                     EMAFLSDDEIERLLERLEGDARRVAILCLSTGARWSEASELRGEHIVGNRVTFFNTKN
                     GKSRSVPVADSVVPLIKTRRTGLLYQVDYLKFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAINFNPLAESVHKLSID"
     gene            complement(14985..15326)
                     /locus_tag="ACHKAY_002993"
     CDS             complement(14985..15326)
                     /locus_tag="ACHKAY_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5701641.1"
                     /translation="MVILSIDYAKKLRQIRKAEGLTQKQFADITGLSLATIRNYESGQ
                     KNARAKIVEAVLQVDRFEKYMLWLIKDKTLPVAGQIAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSGRNVG"
     gene            15431..15952
                     /locus_tag="ACHKAY_002994"
     CDS             15431..15952
                     /locus_tag="ACHKAY_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFO5701642.1"
                     /translation="MRDFIADKELSDHQPLQESDALSGSIDAANDDEHEENKKKRSYK
                     KGATLRLDGPIAGLCSLEKGAAYIGLTKSALRVAIHRGQMPGHKTRTNPEDENSDGVW
                     WFNAKEWDKLADELPEHEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     SSKLNQLRNNRGN"
     gene            15957..16379
                     /locus_tag="ACHKAY_002995"
     CDS             15957..16379
                     /locus_tag="ACHKAY_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701643.1"
                     /translation="MKTNTSDFTITISKNDQALIDNLKYLAEKHNNGRIAINLRHITG
                     VTIVTSYWHLYLIAKSVLENKDSVVNGRDVDTLPAQIKPIAYQLRNRFKNEGGIIFDF
                     PAAVAVSFSREDILHSFRFALSGDETLWQLEDAGSSPA"
     gene            complement(16386..16577)
                     /locus_tag="ACHKAY_002996"
     CDS             complement(16386..16577)
                     /locus_tag="ACHKAY_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701644.1"
                     /translation="MSEDKKVVNLLSDLSAALDNINPPRVKTTGDKDALNAEIHILLA
                     LVTDKIQRKYPPVTQLVDF"
     gene            16713..17063
                     /locus_tag="ACHKAY_002997"
     CDS             16713..17063
                     /locus_tag="ACHKAY_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO5701645.1"
                     /translation="MKQQRNSSQPRFRNGAERHANRFATSASRSNSRYSLSETHATPD
                     GYPVKQIGEHTWLIEKAGIVVHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            17074..17361
                     /locus_tag="ACHKAY_002998"
     CDS             17074..17361
                     /locus_tag="ACHKAY_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701646.1"
                     /translation="MTKDHAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEAEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            17373..17615
                     /locus_tag="ACHKAY_002999"
     CDS             17373..17615
                     /locus_tag="ACHKAY_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO5701647.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            17612..17725
                     /locus_tag="ACHKAY_003000"
     CDS             17612..17725
                     /locus_tag="ACHKAY_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701648.1"
                     /translation="MSIFISWLVLIISVICAIGIMRIIHSVKKIERFFSDE"
     gene            17819..18229
                     /locus_tag="ACHKAY_003001"
     CDS             17819..18229
                     /locus_tag="ACHKAY_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701649.1"
                     /translation="MNAKEKGIITALKNISETANRAIQDATNAGMIGPATTAMMIARV
                     TAEAAEIIEKQDAELTVLRTQSVTELDLSNTGRLIYTIGSEPQQYTIIVGLQDKYLIT
                     PHPIRGSEILTNLRLIERSQVAFIDDAQRTVFNA"
     gene            18254..18457
                     /locus_tag="ACHKAY_003002"
     CDS             18254..18457
                     /locus_tag="ACHKAY_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701650.1"
                     /translation="MAIKRFSVIRFTSRGREYEVDERLIKTLDRHRSQPDAHHIYLTD
                     DTYFCATNVVQVNLIRQVQESRR"
     gene            18454..18699
                     /locus_tag="ACHKAY_003003"
     CDS             18454..18699
                     /locus_tag="ACHKAY_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO5701651.1"
                     /translation="MTILDYITANPGCSGGEIAAALNTPTTAINAELRRLWRDGLVIR
                     EERKTGGRFSYQVNPMPFGCGNPLTHMFNQLLKEARA"
     gene            18696..18995
                     /locus_tag="ACHKAY_003004"
     CDS             18696..18995
                     /locus_tag="ACHKAY_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5701652.1"
                     /translation="MSTINHQELRELATDLQRMATHQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLTLHHEIADFCAPLGSPGEPETPEAMQRELLQRIDKVFDFFLNQ"
     gene            19007..19210
                     /locus_tag="ACHKAY_003005"
     CDS             19007..19210
                     /locus_tag="ACHKAY_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701653.1"
                     /translation="MNKKTWFRAYMWALVCVLVSLILYAGLLPRMISSDSSFLVLLGI
                     FIAMLYPAGVVRLFSKYIKEIKQ"
     gene            19207..20037
                     /locus_tag="ACHKAY_003006"
     CDS             19207..20037
                     /locus_tag="ACHKAY_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO5701654.1"
                     /translation="MKKFKLFQIIPLFAAILLVGCDRVEPGNVGIKVNKLGDDKGVGE
                     VVGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTT
                     VFQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDSALKDIQEEMT
                     PIGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAG
                     QADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK
                     "
     gene            20406..20636
                     /locus_tag="ACHKAY_003007"
     CDS             20406..20636
                     /locus_tag="ACHKAY_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO5701655.1"
                     /translation="MADRKQHRAIAARRHIQTEINRRLFRAFRVAQIMHINMLHERSH
                     ALSNSYSASVFSYLADDLRELQQLIQQQNKLH"
     gene            20709..21074
                     /locus_tag="ACHKAY_003008"
     CDS             20709..21074
                     /locus_tag="ACHKAY_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701656.1"
                     /translation="MILANDFLEYLLNTERDLAVRVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHHYADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            21081..>23194
                     /locus_tag="ACHKAY_003009"
     CDS             21081..>23194
                     /locus_tag="ACHKAY_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO5701657.1"
                     /translation="MTAEYIRDWQQPRHAMGREGTGIPAPESALSSWLDAYRAENERR
                     QEIADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDHRWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMEVALKA
                     YQMLARMALHLHTVPPHYEALTTDKDRRNEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDRAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSHAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSAHENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPA"
BASE COUNT         6244 a         6044 c         5472 g         5434 t
ORIGIN      
        1 aaaaaccatg cgagttacgg gcctataagc caggcgagat atgatctata tcaatttctc
       61 atctataatg ctttgttagt atctcgtcgc cgacttaata aagagagagt tagtgtgaaa
      121 gctgacaacc cttttgatct tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg
      181 ggtgtctata aagcaacgaa acatccgctt aagactttct atctggcgat taccgccggt
      241 gttttcatct caatcgcatt cgtcttctat atcacagcaa ccactggcac aggcacaatg
      301 cccttcggca tggcaaaact ggttggcggc atttgcttct ctctggggct gattctttgt
      361 gttgtctgcg gagccgatct ctttacttcc acagtgttga ttgttgttgc taaggcgagt
      421 gggcgcatca cctggggtca gttggcgaaa aactggctaa atgtctattt tggcaacctg
      481 gtcggcgcac tgctgtttgt acttttaatg tggctttccg gcgagtatat gaccgcaaat
      541 ggtcaatggg gactaaacgt cctacaaacc gccgaccaca aagtgcacca tacttttatt
      601 gaggccgtct gccttggtat cctggcaaac ctgatggtat gtctggcagt atggatgagt
      661 tattctggcc gtagcctgat ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt
      721 gttgccagcg gttttgagca cagtatcgca aacatgttta tgatcccgat gggtattgta
      781 atccgcgact tcgcatctcc ggaattctgg accgctgtcg gttctgcacc ggaaaatttt
      841 tctcacctga ccgtgatgaa cttcatcact gataacctga ttccggttac gatcggtaat
      901 attatcggcg gtggtttgtt ggttgggttg acatactggg tcatttacct gcgtgaaaac
      961 gatcaccatt aatggttgtc gaagtacgca gtaaataaaa aatccactta agaaggtagg
     1021 tgttacatgt ccgagcttaa tgaaaagtta gccacagcct gggaaggttt taccaaaggt
     1081 gactggcaga atgaagtaaa cgtccgtgac ttcattcaga aaaactacac tccgtacgag
     1141 ggtgacgagt ccttcctggc tggcgctact gaagcgacca ccaccctgtg ggacaaagta
     1201 atggaaggcg ttaaactgga aaaccgcact cacgcgccag ttgactttga caccgctgtt
     1261 gcttccacca tcacctctca cgacgctggc tacatcaaca agcagcttga gaaaatcgtt
     1321 ggtctgcaga ctgaagctcc gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg
     1381 atcgaaggtt cctgcaaagc gtacaaccgc gaactggatc cgatgatcaa aaaaatcttc
     1441 actgaatacc gtaaaactca caaccagggc gtgttcgacg tttacactcc ggacatcctg
     1501 cgttgccgta aatctggtgt tctgaccggt ctgccagatg catatggccg tggccgtatc
     1561 atcggtgact accgtcgcgt tgcgctgtac ggtatcgact acctgatgaa agacaaactg
     1621 gcacagttca cttctctgca ggctgatctg gaaaacggcg taaacctgga acagactatc
     1681 cgtctgcgcg aagaaatcgc tgaacagcac cgcgctctgg gtcagatgaa agaaatggct
     1741 gcgaaatacg gctacgacat ctctggtccg gctaccaacg ctcaggaagc tatccagtgg
     1801 acttacttcg gctacctggc tgctgttaag tctcagaacg gtgctgcaat gtccttcggt
     1861 cgtacctcca ccttcctgga tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc
     1921 gaacaagaag cgcaggaaat ggttgaccac ctggtcatga aactgcgtat ggttcgcttc
     1981 ctgcgtactc cggaatacga tgaactgttc tctggcgacc cgatctgggc aaccgaatct
     2041 atcggtggta tgggcctcga cggtcgtacc ctggttacca aaaacagctt ccgtttcctg
     2101 aacaccctgt acaccatggg tccgtctccg gaaccgaaca tgaccattct gtggtctgaa
     2161 aaactgccgc tgaacttcaa gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg
     2221 cagtatgaga acgatgacct gatgcgtccg gacttcaaca acgatgacta cgctattgct
     2281 tgctgcgtaa gcccgatgat cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac
     2341 ctggcgaaaa ccatgctgta cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag
     2401 gttggtccga agtctgaacc gatcaaaggc gatgtcctga actatgatga agtgatggag
     2461 cgcatggatc acttcatgga ctggctggct aaacagtaca tcactgcact gaacatcatc
     2521 cactacatgc acgacaagta cagctacgaa gcctctctga tggcgctgca cgaccgtgac
     2581 gttatccgca ccatggcgtg tggtatcgct ggtctgtccg ttgctgctga ctccctgtct
     2641 gcaatcaaat atgcgaaagt taaaccgatt cgtgacgaag acggtctggc tatcgacttc
     2701 gaaatcgaag gcgaataccc gcagtttggt aacaatgatc cgcgtgtaga tgacctggct
     2761 gttgacctgg tagaacgttt catgaagaaa attcagaaac tgcacaccta ccgtgacgct
     2821 atcccgactc agtctgttct gaccatcact tctaacgttg tgtatggtaa gaaaactggt
     2881 aacaccccag acggtcgtcg tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac
     2941 ggtcgtgacc agaaaggtgc tgtagcgtct ctgacttccg ttgctaaact gccgtttgct
     3001 tacgctaaag atggtatctc ctacaccttc tctatcgttc cgaacgcact gggtaaagac
     3061 gacgaagttc gtaagaccaa cctggctggt ctgatggatg gttacttcca ccacgaagca
     3121 tccatcgaag gtggtcagca cctgaacgtt aacgtgatga accgtgaaat gctgctcgac
     3181 gcgatggaaa acccggaaaa atatccgcag ctgaccatcc gtgtatctgg ctacgcagta
     3241 cgtttcaact cgctgactaa agaacagcag caggacgtta ttactcgtac cttcactcaa
     3301 tctatgtaat tagatttgac tgaaatcgta cagtaaaaag catacaataa aggctccacg
     3361 aaagtggggc cttttttagc acgagagcct tttttgtcag ctatctatac tttaaggtga
     3421 ctgccaaaac agactcgacg tagccttcga gctgcgcacc aacacggcct cagatgggcc
     3481 acatctggag aaacaccgca atgtcagtta ttggtcgcat tcactccttt gaatcctgtg
     3541 gaaccgtaga cggcccaggt attcgcttta tcaccttttt ccagggctgc ctgatgcgct
     3601 gcctgtattg tcataaccgc gacacctggg atacgcatgg cggtaaagaa gttaccgttg
     3661 aagatttaat gaaagaggtg gtgacctatc gccactttat gaacgcttcc ggcggcggcg
     3721 ttaccgcatc cggcggtgag gcaatcctac aagctgagtt tgttcgtgac tggttccgcg
     3781 cctgcaaaaa agaaggcatt catacctgtc tggacaccaa cggttttgtt cgtcgttacg
     3841 atccggtgat tgatgaactg ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga
     3901 tgaacgacga gatccaccaa aatctggttg gagtttccaa ccaccgcacg ctggagttcg
     3961 ccaaatatct ggcgaacaaa aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct
     4021 ggtctgacga tgacgattca gcgcatcgcc tcggtgaatt tacccgtgat atgggcaacg
     4081 ttgagaaaat cgaactcctc ccctaccacg agctgggcaa acacaaatgg gtggcaatgg
     4141 gtgaagagta caaactcgac ggtgttaaac cacctaagaa agagaccatg gaacgcgtga
     4201 aaggcattct tgagcagtac ggtcataagg taatgttcta atttccccgt aaagcggcaa
     4261 ctttattgag ttgccgcttt tttattccgc gcttcattct gctacagcgt ttttaacaca
     4321 ttttatagtg catctaccat atcccgtacc tgagataata acgactgata atagctcgca
     4381 tgaagttctt gcccatcgaa taccactgta ttacacaaga aagtgacatc ggcatcttca
     4441 ataccaagat aactgcacat atttttcaaa taatcgctta tatttttttc ccagcccatc
     4501 tggacaaatg attctttgtc tcctccaacc agcgccaccc aacgaacttt attgaatggt
     4561 aatttgtgcc catctccata agccagccca ttattccata ctctgtcaat atatcctttt
     4621 agcattgccg ggaagctata ccaccagaga ggaaaaacca ccactaacgt gtcatgttca
     4681 agcagctctg aataaagctg atgaacctct ggagaataac gtttatccat attcttccag
     4741 tctggttcat cttccggcgt catcactggg ttgaaattac gccgatataa atcgagttcc
     4801 gtcacctgta ttttccgctc cattgcccgc tgatggatcg catcagcaat atttgcggtc
     4861 aatgaatcat gacgaggatg ggcccatacc aaataaatac gttcagactg cataatttta
     4921 cctccacgtt aagttctgga gcgcacttta cgcaggcggc ataatcctgt aaacgtgatg
     4981 attcaggatg agttaatgaa ggattttcac taatgcggat gaatatgtct gactttgcca
     5041 ctttctttgc cgtcgcccgt aatcaaagct ttcgtgcagc gggcgatgag ttaggcttat
     5101 cctcgtccgc cattagccat agtattaaaa cactggaaca acgtcttaaa attcgcttat
     5161 tcaatcggac aacccgaagc gtatcgttaa cggaagcggg ttctaatctc tatgaacgat
     5221 tgcgcccagc tttcgacgaa attcagatca tgttagatga gatgaatgat tttcgtctga
     5281 caccgacagg aacgctcaaa atcaatgccg cccgggtagc tgcacgtata tttttaatgc
     5341 ctttactggt tggtttcaca cgtgaatatc ctgatattaa agtcgagctc acaactgacg
     5401 attctcttgt cgatatcgtc caacagggct ttgacgctgg cgttcgactt agtggcatcg
     5461 tcgaaaaaga tatgatcagc gtggcgatcg gcccaccagt gaaattatgt gtagcagcaa
     5521 cgccagagta ctttgcaaga tatggcaaac cccgccatcc tcatgatctg cttaatcatc
     5581 aatgtgttgt ttttcgttat cccagcggca aacctttcca ctggcaattt gcaaaggagc
     5641 tggaaattgc tgtcgcagga aatatcattc tggatgatgt tgatgctgaa ctggaggccg
     5701 ttttgatggg tgctggtatc gggtatctgc tctatgaaca gatcaaggag taccttgata
     5761 ctggtcgttt agaatgtgta ctgggagact ggagcacaga gcgtccaggc ttccagatct
     5821 attatccaaa ccgccagtat atgtcctgcg gcttgcgcgc atttctcgat tatgtaaaaa
     5881 cgggtcagat ctgccagagt cagcgtcacc gaccacaata attatttact attcgcttta
     5941 cgcgccagca gcgggaaaat cagtcctaat cctaccagca caaacggcgt tgcaacattc
     6001 agcgccaact ggaaggtcca ctctgcagtg aaggcttcca ttttcgggaa gatccccgtc
     6061 aaacaggcaa aggcggtaaa ggcaaaacac caaatcccga cggtcattgc cagaggctta
     6121 ttacgaataa agacatattc tggtttatat ttctgcgcca ggcgaacgac tgcaataaat
     6181 gcaacaaata cccataaata acgcagcggc atcactaccg agttaagatt caacagccat
     6241 ttatagagat tgttcatatc accgatgccg agagtcggca gcataatcag aattgccacc
     6301 agcaccaggg tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa
     6361 ctggctggaa tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc
     6421 gagaatacca gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa
     6481 gtattaccca tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttagtc
     6541 attaagtcat ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga
     6601 atggcacaaa ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct
     6661 gggttgcgcg tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc
     6721 agcattgaaa tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta
     6781 atgtttgtgg tcgcaatatg cacttcagta atcgcagacg cggttaccgc catcgccaca
     6841 tacaggagcg acataacaaa catcgcaatc cctgccacag aaccgacgat tttcagcgat
     6901 ttcataccgc gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt
     6961 aacgctacga ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc
     7021 gcaatcagaa ttgcctgggg tttttgtgcc agaaagggaa tatgcaccac ccagtaggtc
     7081 cacgcggcga gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg
     7141 cccttcccat ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa
     7201 tagagtgcaa agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta
     7261 ttaacaacgt tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat
     7321 cgcaactgtt tttcctgaac attcccagcc atagacaaat ccttcgccaa aattaaaggg
     7381 gcatctgtaa aacgagggat tatgcgcggc aaataagaag tgcggaaata gcgaaatgca
     7441 aaattataac ctgctgcaca ttttattttt aaacgaataa aatttggaat aaagcaagtc
     7501 agatagaaga aaaaaaaagc agagtgtgaa acagtaagtt cacactctgc ttgaattcat
     7561 ttacacgtga gcaacgggtt tcggcgtatg accggcgttg cgcagcagca tcagcaaata
     7621 gataaacgat acgctggcga tcatgataaa caataaatta tcggagaaat tctgcattag
     7681 catagcggta aatgacgggc caagcagact tcccacagta tagctcaaca gtaaggcctg
     7741 gttcatcgcc accagttgat gatgttcaac tttctcgcaa gcccatgcca tcgccaccgg
     7801 atatagcgta aagccagcgg caccgaggat gaataacgct ggggccatcg ccgcctggct
     7861 aagcatcgcg atactgccga gaatgacgac aaagacctga acacgcaaca ccagcagtcg
     7921 accaaactta tccgccagac gtccaatcgg ccattgtcca aggatacccg cactgaccag
     7981 taccgccatc cagaaaccaa tgctggcatt gctcacccct ttgtgattga ggtacagcgg
     8041 catcaggcca tatagagatc ccagaacgat tcctgagata atgcagccat tcacgccaag
     8101 ccgcgcctga cggagtttta gcattgacgt aattgacgtc gaatcatggt tttcagcctg
     8161 ctgattcagc acacgcgtaa acaacagcgg taagatccct gccaacgtca aacctgtaac
     8221 ccacggcaat acggacatca gctcggttga aactttgctg accagtaact ggcctaaaaa
     8281 cgttcccacg taataaacca tcatatacgc agcaagcaaa cgcccacggt tacgtgacgt
     8341 cccactgcac atcagcgcgc tctcaacaac cacccaaatc atggcacagc cgacgcccgc
     8401 gacaaaacgc caagccaacc agctccagaa tccaatcatc aggccaaggc cggcacagcc
     8461 agcggcaaaa atgaaggagg ccagataata gctgcggtta aagccaatgc gcttaatgac
     8521 ataccctgtc agcaatgtac cgacaaggtt gccggtaaaa taggatgagc tgacaacgcc
     8581 tacctgccat gtggacatgt gttcctgggc gagccaaagc ggcacgagtg tatttaacac
     8641 cgcgatcgcc agagtcaaca aaagcaggcc agacagcaaa agcatgacag gccgggtata
     8701 cgtggacatg aattaaaacc gtgaggaagt tcaaatttca tgcgcatcat gccaccgcca
     8761 aaaacattgt caatgggtga aaatacgcgc atatcgcagc ggcggtatta tcgatttata
     8821 ttactcattc ataacgctaa tgctgaagag atgattaatc tcatctgatt gtttttattg
     8881 atctaacaca aatttcatac gcttcgttca gtcgaaaaat ttcatggtca cagcaaactc
     8941 atatttcccg tctatgcttt cagagcaatg catcacgtct gaccgatttc agacgtacca
     9001 ctgactctcg aaaggagaag aggatgacca aaccgtatgt tcgtcttgat aaaaatgatg
     9061 ctgccgtttt gcttgttgat catcaggctg gtttactttc ccttgtacgg gatatcgaac
     9121 ccgataagtt taaaaataac gtgctggcgc tgggtgacct ggctaagtat ttcaacttac
     9181 ctactattct taccaccagt tttgaaaccg gtcctaacgg tccattagtt ccagaactga
     9241 aagcacaatt ccccgatgca ccttacattg ctcgcccggg aaatattaac gcctgggata
     9301 acgaagattt tgtaaaagca gtcaaagcga caggtaaaaa acagttaatt attgccggtg
     9361 tggtaaccga agtttgcgtg gcattcccgg cgctttcagc cattgaagaa gggtttgatg
     9421 tctttgttgt gactgacgcg tcagggacct ttaatgaaat tacccggcat tcggcatggg
     9481 atcgcatgtc gcaagcaggt gcgcaattga tgacatggtt cggcgtggcg tgcgagctgc
     9541 accgtgactg gcgtaatgat attgaaggac tggcgacgtt gttctctaac catattcctg
     9601 attatcgtaa tttgatgacc agttatgaca cgttaacgaa gcagaaataa aagagaaccg
     9661 gggccgaaac cccggttgtt actgattagc ttgcgacggc catccccacg gtcatatgca
     9721 aaccatagaa tacgccgcga ccgattaatt cccctgccag aagcagaatg aatgagacgg
     9781 atagtagcgg aaccgcaggc tgataacctt ttagctgtgg tgcaatccac aagcacaggg
     9841 caacggccaa aagcacgatc cgccaggaca tcagcgcacc atagtccggc accagcgctg
     9901 cggcctgctg cacagaacta tgaatagttg ccagctctgt gccctgcatc actgacatca
     9961 cgccactcac caccagtgcc agtacagaaa tggctggcag cagacgcatc gcccagccat
    10021 cgacgccagc cagactcaac aacaggtaac ccagtaacgg gccgcccata aacatcgtca
    10081 ggaagaagcc catcggcgtc cagatgctgt accaggtcgg aacggtatca atgctgttat
    10141 acacacgcac catcatccag acaaagatga cgccaagaac catcgtcact atcagccaca
    10201 gcgtacgcaa tgccggtgac agctttttca gcattgccag caaccagccg atgccgccta
    10261 cggcaaaaaa gatcgaaccg ctggcgattt cgttactgag tgctgaagcc cctacccggt
    10321 tgagcgagtt aaaagcgcgc attggtgaac caagatggag catagaggcg ataaagccaa
    10381 tgcccatcag cacccataaa ccaaacatgc aggcgataac acgctgctgg gcttctgctc
    10441 gcaggtcgcc tttgagcagc gccaaagcca gaacgataaa accacctgct acacattgcc
    10501 cgaagaccgt gaagatcatc agcggccatt catgccatcc acttcccatc tcacacctcc
    10561 ttcgggtttg ccagatagcc agtggtatcc ccggtcgggc ggctattggc attgggtttg
    10621 atcacaatat tcggtttggt aaagtgagct cgcggcaacg gcgcaacggc cgccagatcg
    10681 ccatgttttt tacgcagctc gtcgataggg ccgaaatcca gcgcccgcag cggacaagat
    10741 tcaacacaga tcggcttttt accctccgca acacggtcat aacaaccatc gcatttggtc
    10801 atatggcctt tcgtttcgtt atattgcggt gcgccatacg ggcaagccat atggcagtag
    10861 cggcagccaa tgcacacatc ttcatcgacc acaacaaaac catcttcacg tttatgcatc
    10921 gcaccgctcg ggcagacttt agtacaagcc ggatcttcgc agtggttaca tgaaatcgac
    10981 agatagtagg caaacacgtt ctggtgccag acaccgttat cttcctgcca gtcgccgcca
    11041 gcatattcat aaatgcggcg gaagctgact tctggcgtca aatctttgta gtctttacag
    11101 gccagctcgc aggttttgca accggtgcaa cggctggaat caataaaaaa tccatactgg
    11161 gttgtcatcg gttactcctt acaccttttc aacctgaaca aggtttgtat gtgacggatt
    11221 ccccttagcg agaggagacg gacgttgagt ggtcagtacg ttgatacaac cgcccttatc
    11281 gacacgtttt gtatccgggt cataccaggc accttcaccc agtgcgacca cacccggcat
    11341 cattcgtggc gtcactttcg cctcaatatg aacctcacca cgatcgttaa agatcctgac
    11401 tttatcgccg ttgttgatac cgcgtttttg cgcatcaagc gggttgatcc acatttcctg
    11461 acggcaagct gctttcagca catcaacgtt gccgtaagtt gagtgaacgc gagatttata
    11521 gtggaaaccc gtaagctgca gcggatactg tttgttcagc ggatcctgat aactttcaaa
    11581 gcccggcgtg tagatcggca gtggatcgat cacatcgcct tctggcaatt cccaggtagc
    11641 ggcaatgtca gccagcgcct gcgaataaat ctcaattttg cccgatggcg tagtcagtgg
    11701 gtttgcctgc ggatcttcac ggaaggcttt ataagcaacg tgatgccctt gtgggtcgcg
    11761 ctttttaaag atcccctgct tgcggaactc ttcaaacgtt ggcagttcag gaatcgcttc
    11821 ccgcgactgg gcatacagat gccgcatcca ctcttcctgg gtacggcctt cagtaaactg
    11881 ttgctcaacg ccaagacgtt ttgccagttc gctggtcatt tcatagatgg tcttacattc
    11941 aaagcgcggt ttaatcacct gatcgttgaa aatcacgtaa gacatattcc cgcaggatgc
    12001 atccagtgca aagtccatct gttcggaagc ggtgcagtca ggcagcagga tgtcagcata
    12061 tttcgccgat gaggtcatgt ggcagtcgat aaccacaatc agctcgcact tcttatcatc
    12121 ctgaaggatt tcatgggtac ggttgatttc agaatgctgg ttaatcaggc agttaccggc
    12181 atagttccag atcattttga tcggcacatc cagcttatct ttcccgcgta caccatcacg
    12241 cagcgccgtc atttccgggc cacgttcaat ggcatcggtc cacataaaca tcgaaatgct
    12301 ggtctggatc gggttttcca aggtcggcat acggacaaac ggtaagctgt atgaaccttc
    12361 acgcgcgccg ctgttgcctc cgttaatacc aacgttaccg gtcagaatcg ccagcatcga
    12421 gatagcacgg gttgcgattt caccgttagc gtgacgctgc gggccccatc cctggctgat
    12481 aaacgccggt ttggtactgc cgatttcacg agccagtttg atgattttgt ctgccggaac
    12541 accggtgatt tgtgaggccc attccggtgt tttagccacg ccatctggcc cttcacccag
    12601 aatataagct ttatagtggc catttttcgg cgcactggct ggcagggttt tctcatcgta
    12661 gccaacgcaa tatttatcga ggaatgcctg atccaccagg ttttcagtga tcatgacgta
    12721 cgccagaccg ttaaccagtg cggcatctgt tcccggacga atagggatcc actcatcttc
    12781 gcgcccggca ccggtgtcgg tatagcgcgg atcaatgatg atcatgcggg cattagattt
    12841 ctggcgtgcc tgttcaagat agtaagtcac cccaccgcca ctcattcgcg tttcgccagg
    12901 gttattacca aacagcacta ccagcttact gttttcgata tccgacgggc tgttgccatc
    12961 tgcccagccg ccgtaggtat agttcaaacc ttccgcaatt tgcgcggagg agtagtcgcc
    13021 gtaatggttc agatagccgc cgcagcagtt catcagccgc gcgaccaggg tatttcccgg
    13081 cggccaggag cgggtcatgg tgccgcccag cgtaccggtg ccatagttca gatagataga
    13141 ctcgttgccg tactctttga tcaggcgctg catattggtc gcgatgatgt cgtaggcttc
    13201 ttcccagcta atgcgctcga atttgccttc accgcgcgcc ccgactcgtt tcatcggata
    13261 tttcaggcgg tccggattgt agacacggcg acgcatggaa cgcccacgca ggcaggcgcg
    13321 aacctggtgc aggccgtcgt aattgtcatc gccggtattg tccgtttcga catatttgat
    13381 ttcaccgtcc acgacgtgca tacgtagcgg gcagcgacta ccacagttaa ctgtacaggc
    13441 gctccagata accttttcgt ctgattttgt tggaatggcg ctatcgacag cgtgcgcaat
    13501 ccgactaaaa ggtaatgtta atgcgctgct ggccattgcc aggccgccga tcgctgtcgt
    13561 ttttaccaaa ccacggcgac tcacctcagc agccaatacc gcatcaggga ttttcgtttt
    13621 cataatggct cactcaagct tgctcacgat tgattccagt aacaaaccgt attgtgtaat
    13681 tatttctata gcagctcgct atataggctt gtatacatcg aataatttga aaatcaaaga
    13741 gataggtaga ttttcactcg aaagtagtat tacgtgtaag tgaggagtaa ttattgttcg
    13801 gtatcaaagg gagacaaaaa aagcgcccgc aggcgcttta acgtttcttg ctccccctga
    13861 tgatttactt atttttcaac gaataagcca aaacaggggg aacaaattaa cacaaattat
    13921 aactaatcga tggacagttt atggacactt tcagccagcg gattaaagtt gatcgcgtcc
    13981 tgtaaaaagt ccggcgaaaa atgagcgtac gtcattgttt gctgaatcgt tgcgtgcccc
    14041 aggattcgtt gcaacgtaac aatatttccg ccattcatca taaagtgtgt ggcaaatgta
    14101 tgacgcatga catgcgtagc ctgccccttc ggcaaatccg gtttcacctc ctgaagaatc
    14161 tcccggaact tcagataatc aacctgatac aataaccccg ttcgacgggt tttaatcaga
    14221 ggcacgaccg aatccgccac aggaaccgaa cgggattttc cgtttttggt gttaaagaac
    14281 gtcacccggt tgccaacgat atgctctcca cgtagttccg atgcttcact ccatcgggca
    14341 ccagtagaaa gacacagaat ggcaacacga cgcgcatcac cctccagtcg ctcaagaagc
    14401 cgttcaatct catcatcaga aagaaaggcc atctctgtgt tctgaacttt cagttttcgt
    14461 ataccgcgta cagggttcgc gttgtgaaag acctcagctt caatcagcac cgtgaacatg
    14521 gtagacagta cgcataagtc acgattaata ctggacggca ttaatccggc ctgtaatttc
    14581 tctgaccgat attcaagcat aaattttcgc gtcatctgac tggcgcgagg atctcccatt
    14641 tcacgaacaa ccttccctag cctaaccctg tagctatccc cgtaagcctg attacgccca
    14701 tcaagcatcc accacgcatc aagcagctct gataatcgcc gctgatcagc tggcttagcc
    14761 tgccatggct tgtcatgaaa gttctgtaac acatacttct caaactcctg agcctttgat
    14821 ttcagggcaa agatttttcg aacccgcttt ccttccgccc cctgcggtct gacatcgacc
    14881 tgataacgtc cgtctttgag ttgcttaatc gacatgatta gcccctccaa cggataaatt
    14941 aactaaacac tccttgcgat atgtaattcg ctcaatgtgt agagtcagcc aacattgcgg
    15001 cctgatcggg gtgatttttg ggtaataccg attgaaacct gatcgccctc cgactgaata
    15061 gatccatcaa gagagagagc cggagcaatc tgcccggcga caggtagcgt cttatctttt
    15121 attagccaca gcatgtattt ttcaaagcga tcgacttgca gaaccgcctc aacgattttt
    15181 gctctagcgt tcttttgccc cgattcgtaa ttccttatgg ttgccaaaga caacccagta
    15241 atatccgcaa actgcttttg tgttaaccct tccgccttac gtatctgacg taactttttt
    15301 gcatagtcta ttgacaagat aaccatttga gtattaacct ctcgcacaaa gataaccatt
    15361 tgattatctt tacaagccaa aacaaaccat cacaaggcgc gacaagcgca ccaacgcaag
    15421 aggataacaa atgcgggact tcattgccga taaagagtta tctgaccacc aaccacttca
    15481 ggaaagtgac gcactcagcg gatccattga tgccgccaac gacgacgaac acgaagaaaa
    15541 caagaaaaag cgttcctaca aaaaaggagc gactttacgg ctggatggtc cgatcgccgg
    15601 gctttgctct ctggaaaaag gcgcggcata tattgggcta acaaaatctg cactgcgcgt
    15661 agccattcat cgcggtcaaa tgccgggaca caagacgcgc acaaacccgg aagatgaaaa
    15721 ctcagacgga gtatggtggt ttaacgccaa agagtgggac aagttggctg atgagctacc
    15781 agagcacgag cccccagaat ggcacaactg gaaaagttac tggacgtatg accgccagaa
    15841 aaggaagttc tctccagcta ataaagaaga ctgccagacc attaacggta agcgagttta
    15901 tacaggcaga agctcaaaac tgaaccaact cagaaacaac agaggcaact aatcgtatga
    15961 aaacaaacac gtcagatttc acaataacta tttcaaaaaa tgaccaagca ttaattgata
    16021 acctgaaata ccttgcggaa aaacataata acggacgaat tgcaattaat ttacgacaca
    16081 tcacaggagt aacaattgta accagttact ggcatctata tctaatcgct aaatcagtac
    16141 tggaaaacaa agattctgtt gtcaatggaa gagatgtaga tactcttcct gcacaaatca
    16201 aaccgatagc ctatcaattg cgtaatcgtt ttaaaaatga aggtgggatt atatttgact
    16261 ttcccgcagc tgtagccgtg agcttctctc gggaggatat acttcactca tttcgttttg
    16321 ctctatcagg tgacgagaca ttatggcagc tagaagatgc cgggagcagc ccggcataaa
    16381 tcaaattaaa aatcaaccag ttgtgtgaca ggtgggtatt tacgctgaat tttgtcagtt
    16441 actaacgcca gtaatatatg aatctctgcg tttaatgcgt ctttatcacc cgtcgttttt
    16501 accctaggag ggttaatgtt atccagagca gcactaaggt cactcaacaa attaacaact
    16561 tttttatctt ctgacatgaa aacctctaaa caatttaatt aaacataagc caggacctga
    16621 accggccagt ccaagtccta gacacacaaa aaataccaca aatcaataca aggtgacacg
    16681 agtacacctt accgaaggta cagagtttta atatgaaaca acagcgcaat tcttcacagc
    16741 cgcgcttccg caacggtgcg gaacgccacg ctaaccgttt cgctaccagt gcatcacgta
    16801 gcaactctcg ctacagcctg agcgaaacac acgcaacgcc ggatggctac ccagtaaaac
    16861 aaatcggcga gcatacctgg ctgattgaga aagctggaat tgtggtccac aaatgcccac
    16921 gcaatccgtt taccggaaac cgcatttttg cattgagctg tggcgacaat cagtttgggc
    16981 aggatttcac attatacgaa gcacttcgca cggttgatcg tctgcttcgc gggcaaagtt
    17041 ttattaaaca ggctgattta taacgggtgc tttatgacca aagaccatgc acaaggtgta
    17101 tttatccgtt ttattgattt tcgcggtgaa ctgttattac gtgcatccgc tattgacgga
    17161 gtgactccgg cgggtaaaaa cggagccgac gaagccactt acgtttatct gaacggcacg
    17221 cgactgcttg tggaacttcc gtaccagacc gtacgagaaa tcattagcga agctgaaaag
    17281 gcacgccaag ttaatggcga tgaaccctat atcgaaatta tttgtatgga ttcagaagct
    17341 gaaattcaga aagcagatta aagggcgttg cgatgggcaa agaatataaa actctcatta
    17401 ataaagcact tgagcgtttt tattttcgct taagtgcatc aggcgctcat gctgaacgtg
    17461 cagcccgtga ctcattgacc agggcaatcc ggagtctgta tgacgtggct ttttacgctg
    17521 atgatctgga tgcacttaac gaactttccg agctgatctg tgccgcagaa tgcggggaac
    17581 atattgaacc gtataagctg gggaatatcg catgagtata tttatctcgt ggcttgttct
    17641 gattatttcg gtgatctgcg ctattggcat tatgcgaatt attcattcag tgaaaaagat
    17701 tgagcgtttt ttctctgacg aataacagca caaataaaac accagattaa ataagaaaac
    17761 gtgaaaacca tccgaattaa cggaggcatt cacacacgta aataatggag atacaaaaat
    17821 gaacgcaaaa gaaaaaggca ttataactgc gctaaaaaat atatcggaga ccgcaaacag
    17881 agccattcag gatgcaacaa atgcaggaat gattgggcct gccaccacag ccatgatgat
    17941 agcaagagta accgccgaag cagccgaaat tatcgaaaaa caggatgctg aactaacggt
    18001 tctcagaaca caatcagtca ccgaactgga tttgtctaac accggacgcc ttatttacac
    18061 aattggctcg gagccacagc aatacaccat tatcgtcgga ttacaggata aatacctgat
    18121 cactcctcac cccataaggg gatcagaaat tctgacaaat ctccgcctga tagagcgctc
    18181 tcaagtcgca ttcattgatg acgcacagcg caccgtattt aacgcatagg gttactggac
    18241 aaaggggggc gcaatggcaa ttaagcgttt ttccgtcatt cgtttcacat ccagagggcg
    18301 tgaatacgag gttgacgaac ggctgattaa aacgctcgac cgtcaccgtt cgcaacctga
    18361 cgcgcatcac atttacctca ctgacgacac ttacttctgc gccaccaacg tggtgcaggt
    18421 gaatcttatc agacaggtac aggagtcacg cagatgacca ttctggacta catcacagcc
    18481 aatccgggtt gtagcggtgg agaaatcgcc gccgcgctga ataccccaac cacagccatt
    18541 aatgctgagt tgcgccgact ctggcgtgac ggcttagtca tcagagaaga gcgcaaaaca
    18601 ggcggtcggt tctcttacca ggtaaacccg atgccgttcg ggtgtggcaa cccacttact
    18661 cacatgttta accagctact gaaggaagcc agagcatgag caccatcaac caccaggaac
    18721 tacgcgaact ggcgactgac ctgcaacgaa tggcaacgca tcaaaaatta ctggcgtttc
    18781 gcgcaatgct ctcgccatct gccgtgttgg cactactgga tgagctggag cacgccagaa
    18841 ccacagcgcc agccattcgc ctgacgctcc atcatgaaat cgctgatttc tgtgcaccac
    18901 tgggttcacc tggcgaaccg gaaacaccgg aagcaatgca gcgagagctg ctgcaacgca
    18961 tcgacaaggt ttttgatttt ttccttaacc agtaagggat cgcagcatga acaaaaagac
    19021 ctggtttcgc gcatacatgt gggcgctggt atgcgtcctc gtttctctca ttctgtatgc
    19081 aggactcctc ccccgaatga tttcatcaga cagctccttc ctggtattgc tgggcatttt
    19141 cattgccatg ctgtacccgg caggcgttgt tcgccttttc agtaagtaca tcaaggaaat
    19201 caaacaatga agaaattcaa actctttcag attatccctc tttttgccgc catcctgctg
    19261 gttggttgcg atcgtgttga gccaggtaat gtgggcatca aagtcaacaa actgggcgac
    19321 gacaaaggcg tcggtgaagt ggttggtgtt ggccgctact ggactggctg gaataccgag
    19381 gtttacatct tcccgacctt caaacaaatg aagacctacg atgagccgtt cagcttccag
    19441 atgagtgacg gcacaaccat cggctatcac atcggtgtgg cctacaaggt tgatccatcc
    19501 aaagttacta cagtgtttca gacctaccgc aaaggcgtgg atgacattac cgacactgac
    19561 ctgcgccaga agatcgccga tgcactcaac cgactggcca gcaaaatgac caccgataag
    19621 tttatcgacg gtggtaagtc tgaactgctg gattcagcac ttaaagatat tcaggaagag
    19681 atgacaccca tcggtattca ggtaatgagc ctctcttatg tcggtaaacc ggaatatccg
    19741 ccaaccgtta tcgacagcat taacgccaaa gtcacggcaa accagaaaac cctgcaacgc
    19801 gaacaggaag tcaagcaacg cgaagcagaa gccaacatgt tgcgcgcaga agctgccgga
    19861 caggctgatg ccattcgcac aaaagcccag gctgaagccg acgccattcg tttacgcggc
    19921 gaagctctgc gccagaatcc cggcgttatg gagctggaag caatcaacaa atggaacggc
    19981 acgctgccgc aatacatgac cagcaacacc gctgttccgt ttgttccggt gaagtaatta
    20041 aacccggcca gtgaaaatcg ctggccggag cagtatcagg atttttttag tatgccgttc
    20101 tcacaaaaaa accgcttgcc atgccgcaat cagtcaggtt acatttccgc tgcacctcat
    20161 aaaacgggtg ccgggattct caacccgata cagagcaaag cgcataaccg cgcaagcggt
    20221 ttttttgtgc gtactgtatt gccacgtctt tttcgcgtca gaattatggc ggggcgtacg
    20281 gggccgactt cggtcgggcc ggattctttg ctctccggtg ttgagaaccc tgtacgtctc
    20341 gccaccccga gattctcaac tctggatggt gagttatttc tatcaccgag caaagaggcc
    20401 acaccatggc agaccgcaaa cagcaccgcg ctatcgctgc gcgtcgtcac atccagactg
    20461 aaatcaaccg cagacttttc cgcgcattcc gcgtcgcgca aatcatgcac atcaatatgc
    20521 tgcatgaacg cagtcacgca ctatcgaaca gctattccgc ctctgttttc agctatctgg
    20581 cggatgatct gcgcgagctt cagcagctca tccagcagca aaacaaactc cattaattcc
    20641 tgttccgggc ctttcctgca ccttgcggcg ggaggccttc gcacatctgt aacaagagga
    20701 gtgccgcaat gattctcgcc aacgactttc ttgaatacct gctcaacaca gagcgtgatc
    20761 ttgccgttcg cgtgcgtgac cgttatgaca tgtacctgaa atccctgcct gtaccgcagc
    20821 tcgctgacgg aaagattgtt attgatggcc gctacatgat tgacagccac gagggaaatt
    20881 acaggcttta ccgcattgaa ggcggcaccc cgtccgttat tggcatttac cagcgcccat
    20941 cctctgcgat cgtcgatgtg attgcagaca gcatccgcat cacacatcac tatgccgaca
    21001 cagaagacac cgtgctggaa attcagcggc tggctacagt ctgccgcgac accctgaatg
    21061 gcatgacgaa gtaaatcact atgacagcag agtacatcag ggactggcaa caaccgcgcc
    21121 acgcaatggg gcgtgaagga acggggatcc ctgctcctga atccgcgctt tcctcctggc
    21181 tggatgccta ccgggcagag aacgagcgcc gccaggaaat agctgatgcg gcgttctccg
    21241 ccacaccgct gggcaacctg attaataaaa gcctggacgc acaggaaaaa caggacaaaa
    21301 ccatcacact ggcaggagac gccagaaaac aggcacgcgg cgcggtggat gaagccatgg
    21361 cctcgctgcg cctgctgccg tcctatctgc gcgatccgct tattcgccac ctctccttcc
    21421 tgcgcaaaaa acaggaagcc gatcgccgga aaggcaaaaa gagctggcag gcggaacgct
    21481 atgcacgcgg aaccctgcgc aaaatattcg aacgtctgga ccgcaccgat caccgctggc
    21541 tgacaccggg ttatcgttcc cttgccggac gcgaacgcct ggacgatttg ctttacctgc
    21601 cgcagctcaa caaacaccag atacagacgc tggccaccat gacggcggcg atgttcagca
    21661 gcaccttcga aaaactctgc gatggctttg gcgcgactga tggtgagctg accatggagg
    21721 tggcactgaa ggcgtatcag atgctggccc gcatggcgtt acacctgcac accgtgccgc
    21781 cgcattacga agcgctgaca acagacaaag accggaggaa cgaaccggac acggagctgc
    21841 tgccgggcgc aatccttcgc ctgacctgtg cggaatggtg gaaacgcaaa ctgtggctgt
    21901 tacgttgcga gtggagagaa gaacaactcc gcgccgcctg tctggtttcc agaaaaacat
    21961 cgccctatct gagccaggac gcgttaagcg agtttcgcgc acagcgcgag aagacacgcg
    22021 atttcctgaa aagtttcatg ctggaaaacg aagacgggtt cacgattgat ctcgagacag
    22081 tgtattacgc gggagtaagt aacccggttc accgtaaggc agaaatgatg gccaccatga
    22141 agggactgga acttctggcc gaagcccgtg gcgacagagc ggtgtttctg actgtcacct
    22201 gcccgtcaaa ataccacgcc acaacagaga acggtcatcc gaatcccaaa tggaacgggg
    22261 ccaccatgcg cgactccagc gattacctgg ttaacacgtt ttttgcggct gtccgcaaaa
    22321 aactgaaccg cgacggtctg cgctggtatg gcatccgcac ggtggagcct caccatgacg
    22381 gcaccgtgca ctggcatatg atggtctttg ctcatccgga agaaatcgac accattgtgt
    22441 cccacacccg cgatattgcc attcaggaag accgccacga gctgggcgat gacataactc
    22501 cgcgctttaa ggcggagtac gtcgacggct caaaaggcac accaaccagc tacatcgcca
    22561 cctacatcgg aaagaacctg gacagccacg ccgtggatgg catcgacccg aaaacgggca
    22621 agccacgcgt tgaccacgaa accggaaaat caatggccga gagcgtggaa cgcgccatcg
    22681 gctgggcgcg ccttcaccgg gtccgccagt tccagttctt tggtatcccc tcccgtcagg
    22741 tgtggcgtga actgcgccgc cttgccagcc agatggcacg caacccggaa ggcccgcaac
    22801 ggctgaagga tgacgcaatg gacgcggtac tcgctgccgc tgatgccgga tgttttgcca
    22861 cctacattga gaaacagggt ggcgtacttg ttccacgcaa agactacctg attcgcaccg
    22921 cctacgacct cgcagatgag ctgaacgatt acggtgaaca gagtgtacag atttacggga
    22981 tctggtcgcc gctcattggg gagtcttccc gcgtatgcac gcatccggat aactggaagc
    23041 tggtaagacg caaaccggaa gcggaagaca gcgcccacga aaatggtttt gaccttcagg
    23101 gcggccctgc cgccccttgg actcgtggca ataactgtcc ccgtgtacag gaaacggaca
    23161 acaacgggac agaacagccg gaagaacggc cagc
//
LOCUS       DAZFKM010000075        23172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFKM010000075 DAZFKM010000000
VERSION     DAZFKM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..95
                     /locus_tag="ACHKAY_003010"
     CDS             <1..95
                     /locus_tag="ACHKAY_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701658.1"
                     /translation="QEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            223..759
                     /gene="yfdR"
                     /locus_tag="ACHKAY_003011"
     CDS             223..759
                     /gene="yfdR"
                     /locus_tag="ACHKAY_003011"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5701659.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSGLRKCA"
     gene            750..896
                     /locus_tag="ACHKAY_003012"
                     /pseudo
     CDS             750..896
                     /locus_tag="ACHKAY_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            873..1337
                     /locus_tag="ACHKAY_003013"
                     /pseudo
     CDS             873..1337
                     /locus_tag="ACHKAY_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1339..1530
                     /locus_tag="ACHKAY_003014"
     CDS             1339..1530
                     /locus_tag="ACHKAY_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701660.1"
                     /translation="MNGQISIVRPGACDDSEIRMIIRLARGKTITVLITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            1533..2123
                     /locus_tag="ACHKAY_003015"
     CDS             1533..2123
                     /locus_tag="ACHKAY_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5701661.1"
                     /translation="MTTFTNSKLTDEYVSNATLIRLILWADQHNSHYVVAALRELQER
                     RKADSAEPVSQTYKLPVNTPCQDAPAHIWLQTAGVWPEDGELSELTWCSHNQHHDDTL
                     YVRADLVNGNYPATPDGWISCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGD
                     WFNDGKPTWDDGDGEDLYLKEVTHWMQLPEPPQEVK"
     gene            2211..2453
                     /locus_tag="ACHKAY_003016"
     CDS             2211..2453
                     /locus_tag="ACHKAY_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5701662.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            2457..2591
                     /locus_tag="ACHKAY_003017"
     CDS             2457..2591
                     /locus_tag="ACHKAY_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701663.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVAKRMFDVWCHCRG
                     "
     gene            2610..2864
                     /gene="xisR"
                     /locus_tag="ACHKAY_003018"
     CDS             2610..2864
                     /gene="xisR"
                     /locus_tag="ACHKAY_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO5701664.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2898..4184
                     /locus_tag="ACHKAY_003019"
     CDS             2898..4184
                     /locus_tag="ACHKAY_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701665.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTDKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            4205..4906
                     /gene="mlrA"
                     /locus_tag="ACHKAY_003020"
     CDS             4205..4906
                     /gene="mlrA"
                     /locus_tag="ACHKAY_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO5701666.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            4966..5073
                     /gene="yohO"
                     /locus_tag="ACHKAY_003021"
     CDS             4966..5073
                     /gene="yohO"
                     /locus_tag="ACHKAY_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO5701667.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(5054..5785)
                     /gene="yehW"
                     /locus_tag="ACHKAY_003022"
     CDS             complement(5054..5785)
                     /gene="yehW"
                     /locus_tag="ACHKAY_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO5701668.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5790..6716)
                     /gene="yehX"
                     /locus_tag="ACHKAY_003023"
     CDS             complement(5790..6716)
                     /gene="yehX"
                     /locus_tag="ACHKAY_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO5701669.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6709..7866)
                     /gene="yehY"
                     /locus_tag="ACHKAY_003024"
     CDS             complement(6709..7866)
                     /gene="yehY"
                     /locus_tag="ACHKAY_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO5701670.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7873..8790)
                     /gene="osmF"
                     /locus_tag="ACHKAY_003025"
     CDS             complement(7873..8790)
                     /gene="osmF"
                     /locus_tag="ACHKAY_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO5701671.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(9001..11298)
                     /gene="bglX"
                     /locus_tag="ACHKAY_003026"
     CDS             complement(9001..11298)
                     /gene="bglX"
                     /locus_tag="ACHKAY_003026"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO5701672.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11494..13209
                     /gene="dld"
                     /locus_tag="ACHKAY_003027"
     CDS             11494..13209
                     /gene="dld"
                     /locus_tag="ACHKAY_003027"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO5701673.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(13247..14179)
                     /gene="pbpG"
                     /locus_tag="ACHKAY_003028"
     CDS             complement(13247..14179)
                     /gene="pbpG"
                     /locus_tag="ACHKAY_003028"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO5701674.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14353..14940)
                     /gene="yohC"
                     /locus_tag="ACHKAY_003029"
     CDS             complement(14353..14940)
                     /gene="yohC"
                     /locus_tag="ACHKAY_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO5701675.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            15110..15688
                     /gene="yohD"
                     /locus_tag="ACHKAY_003030"
     CDS             15110..15688
                     /gene="yohD"
                     /locus_tag="ACHKAY_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO5701676.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15818..16579)
                     /gene="yohF"
                     /locus_tag="ACHKAY_003031"
     CDS             complement(15818..16579)
                     /gene="yohF"
                     /locus_tag="ACHKAY_003031"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5701677.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16632..18068)
                     /gene="mdtQ"
                     /locus_tag="ACHKAY_003032"
     CDS             complement(16632..18068)
                     /gene="mdtQ"
                     /locus_tag="ACHKAY_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO5701678.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            18292..18375
                     /gene="yohP"
                     /locus_tag="ACHKAY_003033"
     CDS             18292..18375
                     /gene="yohP"
                     /locus_tag="ACHKAY_003033"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO5701679.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(18763..19713)
                     /gene="dusC"
                     /locus_tag="ACHKAY_003034"
     CDS             complement(18763..19713)
                     /gene="dusC"
                     /locus_tag="ACHKAY_003034"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO5701680.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            19952..20350
                     /gene="yohJ"
                     /locus_tag="ACHKAY_003035"
     CDS             19952..20350
                     /gene="yohJ"
                     /locus_tag="ACHKAY_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO5701681.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            20347..21042
                     /gene="yohK"
                     /locus_tag="ACHKAY_003036"
     CDS             20347..21042
                     /gene="yohK"
                     /locus_tag="ACHKAY_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO5701682.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            21172..22056
                     /gene="cdd"
                     /locus_tag="ACHKAY_003037"
     CDS             21172..22056
                     /gene="cdd"
                     /locus_tag="ACHKAY_003037"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO5701683.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            22206..22925
                     /gene="sanA"
                     /locus_tag="ACHKAY_003038"
     CDS             22206..22925
                     /gene="sanA"
                     /locus_tag="ACHKAY_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO5701684.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            22928..23167
                     /gene="yeiS"
                     /locus_tag="ACHKAY_003039"
     CDS             22928..23167
                     /gene="yeiS"
                     /locus_tag="ACHKAY_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO5701685.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
BASE COUNT         5687 a         6085 c         5841 g         5559 t
ORIGIN      
        1 tgcaggaaga aatggctaac gaatttcgtg atctgcttgt tgagaaattt aaagacagca
       61 aagtagaaac ctttattggt actttcaccg cctgatttca ttactgcaaa tgcccctgcg
      121 ggggcattta tggaaacata atttactcaa taatcgccgg atggtgaggg cttcctttta
      181 ccagaattca gcgcggtgca gcgcatatac gtggagaaca aaatgtcatt tattaaaact
      241 ttttccggga agcattttta ttatgacaag ataaataaag acgacatcgt tattaacgat
      301 atcgcggttt ccctttcaaa tatctgtcgc tttgcaggac atctttcaca cttctacagc
      361 gtcgcccaac atgcggtgct ttgcagccag ctggtaccgc aggaatttgc ttttgaagcg
      421 ttaatgcatg atgcaacaga agcatattgc caggacatcc ccgcaccact gaaacgcctt
      481 cttcctgact ataaacggat ggaagaaaaa atagacgccg taatccgtga gaaatacggg
      541 ttacctcctg ttatgagcac gccagtgaaa tatgccgatc tcattatgct ggcaaccgaa
      601 cgccgcgatc tcgggcttga tgatggttct ttctggcctg tactggaagg tatcccggcg
      661 acagagatgt tcaaagttat tccactgtca ccaggccatg cctacgggat gtttatggaa
      721 cgttttaacg agttatcggg gttacgcaaa tgcgcatgaa tgttttcgaa atggaagggt
      781 ttcttcgcgg gaaatgtgta ccgcgagatc tgaaagtgaa cgaaacaaac gctgagtacc
      841 tagtgcgtaa atttgctgaa gctgaggcca agtgcgcggc gctggctgaa cgtatcgaag
      901 agttacagac aaaacctacg ccagattcgt ttggcatcat cggtgaaaat attcgaacac
      961 aggataatcg aataacgtca gaccctatgt tttgtgtgta tcaaaagcgc gaaattgttg
     1021 ttgatgctga ttatgactat gaccggattg tctgggttga tgaagatggc aatgaagcca
     1081 ataaactcca aagtcgtcgt ctcgaactac ttcatgaaaa ctttcgggaa ccaccagaaa
     1141 aatggcggcg cgttgctgtg aaagatattg atgaattcgt tacctgctgt ttcaccgaac
     1201 agggttgtaa agactacctg gcagccaatg gtcacaatct tcgcttgcca tttatatatg
     1261 taaaaagcgg tttcaggaac gctgaatata tcggcataag aacctggctt gctggcattc
     1321 gcatcaaagg agagtgagat gaacggacaa atatcaattg ttcgaccagg agcatgtgac
     1381 gatagcgaga tacgcatgat tattcgtctg gcgaggggga aaacaataac tgttctcatt
     1441 actccagaaa atctcgcatt agcattaaca ggaaagtcag acctgccagt agagctaaag
     1501 ctgcgaaatg ttgagattaa ggtgaaatag ctatgaccac ttttaccaat agcaaactaa
     1561 cagacgaata cgtttcaaat gcaacgttga ttcggctcat tctgtgggct gaccagcaca
     1621 atagccatta tgttgtagcg gctctgcgcg agctacagga gcgccgcaag gctgatagtg
     1681 cagaacctgt aagccaaact tacaagttgc cagttaatac accttgccaa gatgcgccag
     1741 cccatatctg gctgcaaacg gctggagtat ggccagaaga tggcgagtta agcgaattaa
     1801 cgtggtgcag ccacaatcaa caccatgatg acacgctata tgttcgagct gaccttgtga
     1861 atggcaacta tccggcaact ccggatggtt ggataagctg tagtgagcga atgcctgaaa
     1921 tgggagagcg acaatgctat gtgttagcag ctgactttaa aaacaactac ccaccaaaca
     1981 tccccaacac tcaggtcggc gtatatggcg actggtttaa tgatggcaag cccacttggg
     2041 atgacggtga tggcgaagac ctgtatctca aagaggtaac ccactggatg cagctaccag
     2101 aaccgccgca ggaggtgaaa tgatgaattg gcctgaagca ttcaccgcag ttggagttgc
     2161 aatagcggtg gcatttattc tgtattcgct tttccgctgg ggataaagga atgttcgctc
     2221 tgattcaacg tggtcagata tacacggaca gagccggata ccctgtggtg attactcgca
     2281 gtactcagca ctcagtgttc tttcgacgca tggacgggcg ctccggacgg gtacgcattg
     2341 gtgagttcaa caacctgttc gaacatattg accaacagga gtaccgcaaa attctggcgg
     2401 gcactgagca ggaaatgcgc ctgaaaaaat tacgcgcaat gcaacggagg tgatacatgc
     2461 atacggcttt tgagttctgg gttcgcaaga cattcggcaa tcgctacgac ctgacccgtg
     2521 atgtcgacgg cttctactgc cgtgaagttg cgaaacgaat gtttgacgtg tggtgccact
     2581 gccgtggatg aaagttttat gaggttggca tgcagacaat catctatcag ataaccccca
     2641 gcaaatggtg tacggagaga gtccttattg catcaacagg gctaaagccc ggcaccatcg
     2701 agcgggccag aagaaagtca tggatgcagg gaaaagaata ccgccattac gctgtagaag
     2761 gtgatcctgg gcattacagt gaatgcctgt acaacatcga agaaattatg cgatggatcg
     2821 aaaaccagaa acaaccaggt gccaaaaatg caagttccgg ttaacctgtt aatgctcctg
     2881 gacgtctggg aggtttaatg agtaacgcat catacccgac aggcgttgaa aaccatggag
     2941 gatcactccg tatatggttt cactataatg gcaaacgtgt cagagaaaac ctcggtgttc
     3001 ctgacacaga caaaaaccgg aagatcgctg gtgaacttcg cacttccgtt tgttttgcaa
     3061 tcagaatggg gagtttcgac tacgccgcgc agttccctaa ttcccctaac ctgaaacact
     3121 ttggtctggg aaaaagagag ataaccgtta aggcactttc ggaaaaatgg ttggacctta
     3181 agaaaattga gatttgtgcg aatgcactta accgttacca gtcagtaatt aaaaacatgt
     3241 taccaatgtt aggtgaaaaa aaactggttt catccataac aaaagaggat ttacttttcg
     3301 taaggagaga tttgttgacc ggttaccaaa agctttctaa tggaaagact tcttccataa
     3361 aagggcgctc agtggtcacg gtaaactact atatgacaac catagctgga atgtttcaat
     3421 ttgcaacaga taatggttat acctcaggaa acccatttaa cggtctggct cccttaaaaa
     3481 agtccaaggt aaaaccagat cctctcaccc gtgacgaatt tattcgtttt attgaggctt
     3541 gccgtcatca acaaacaaaa aacctgtgga ttctcgctgt atacacgggt attcgtcacg
     3601 gggagttggt atcgctggca tgggaagata tagaccttaa agcaaggact ataaccatcc
     3661 gtagaaatta tacaaaactt ggcgaattca ctccaccaaa aaccgatgca ggcaccggaa
     3721 ggacaattca tctggttcaa ccagctattg atgctcttaa aagccaggcg gaaatgacca
     3781 tgcttggaaa gcaacattct gtagaggtga agcagaggga atatgggaga actgctgtgc
     3841 ataaatgcac ttttgttttt agtcctcagg taacaaaaca gcagcagttg tccggacctc
     3901 actacaaggt tgactccatc agggagtcat ggacaagtat cttaaaacgc gcaggtctga
     3961 gacacagaaa atcgtaccaa tccaggcata cttatgcatg ctggtcactt gccgcaggag
     4021 ctaatcctag ttttatcgca agccagatgg gccacacaaa cgcacaaatg gtattcaatg
     4081 tttacggagc atggatgaaa gacaacaatc acgaacagat agaactcctt aacaaaagac
     4141 tatctgaaag tgtcccatgt atgccccata agaaagcagg gtaaaataaa aacttgcaaa
     4201 atcaattagt ttacccttaa tccctgtcac gttacgcgcg tggcagaggc gttacgggtt
     4261 gctgaaaccg caacggacag acggcggtca tcgactgttc aacgatgccg atattgaccg
     4321 tatccgcgag atcaaacgct ggatcgacaa cggcgtgcag gtcagcaaag ttaaaatgct
     4381 gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc gatcagcaag aaacattact
     4441 gacctacctg caaagcggca atctgcatag cctgcgaacg tggatcaaag agcgcggtca
     4501 ggattacccc gcccagacac tcaccacaca tctgtttatt cctctgcgcc gacgacttca
     4561 atgccaacaa ccgactctcc aggcgctgct ggcgatcctc gacggcgtac tgatcaacta
     4621 catcgccatt tgtctggctt cggcacgtaa aaaacagggt aaagatgcgc tggtggttgg
     4681 ctggaatatt caggatacca cccgtctgtg gctggagggc tggattgcca gtcaacaagg
     4741 atggcgcatt gatgtcctcg cccactcgct caatcaacta cgccctgaac tattcgaagg
     4801 tcgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc gcccaacagc agcaactcac
     4861 cagttggcaa gaacaaggcc atgatatttt cccactcggc atttaatgat tcgttaacaa
     4921 atgcgcttta ctgtacaatc ctttcgttaa cataaggagt gcattatgcg catagctaaa
     4981 attggggtca tcgccctgtt cctgtttatg gcgttcggcg gaattggtgg cgtcatgctc
     5041 gcaggttata cctttatttt gcgtgctggc taagcgcctg caccagcctt tcaaacaggc
     5101 ggtctgcgat gatcgccgcc agtgccacca gtaacgcccc ctggatcaca tacgcggtat
     5161 taaatccgct aagcccgatg atgatgggcg tacccagcgt gctggcccct accgttgagg
     5221 cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg cacgcccgcc agaatcaccg
     5281 gagccgccag cggtagctcg accttacgca gtcgctgacc acgactcatt cccatacctt
     5341 tcgcaacttc tgtcacgctg gcatcgatcg ctcccagccc ggcaagtgtc gcctgcagga
     5401 cgggcagcac accgtaaagg atcaaggcga taatcgctgg ttgcagacca aagccgatca
     5461 ccggaacggc aatcgccagc actgcgacag gcggaaaagt ctgtccaacg gcggcaatag
     5521 tttccaccag tgggcgaaat tccgcgcccc acgggcgagt gacagcaatt ccggcaccag
     5581 tgccaatgat caccgcaaac aaactcgaaa ttcccaccag ccagaaatga gccagtgcca
     5641 gagctgcaaa actttcttgc tgataaacgg gtcgtggcag ttgtgggaac aaggcagcaa
     5701 acagcggctg gctgtaaggc agccagaaaa tcagtgccac aaacaaagca atgagccaga
     5761 acagcggatc gcgcaacatc ttcatacgct tacgcctcca ccagcagatc ctgaaaatgc
     5821 agcgtaccgc aaggctggcc ctgcgtgttc accaccggca gcacctcgca accccgcgcg
     5881 acaaacaggg agagcgcatc gcgtagcgtc atctcttctg ccagtgcctc accttctgcc
     5941 cgttcttcgc gacgcacgta atccgccaca ctacgtaacg aaagcagacg cacacccagt
     6001 tcgctacgtc caaaaaactg gcggacaaaa tcattcgccg gacgagtcag catcgtcagc
     6061 ggattgccct gctgcaccac ttcaccgtga tccatcaata ccagatgttc agccagccgt
     6121 agcgcctcat caatatcatg agtgaccagc acaatagtgc gccccagcaa acggtgaatg
     6181 cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc aaaaggttca
     6241 tccatcagta agacttgcgg atcggcagcc agtgcgcgcg ccactcccac acgttgctgc
     6301 tgaccaccgg aaagctgatg cggataacgc tcacgcaaat ttgactccag ccccagtagc
     6361 gtcattaatt cgtcgatacg atcgtcaatc cgtgcccgcg accatttttg taattgcggc
     6421 acggtagcga tgttttgcgc cacgctccag tgggggaata ggccaataga ttgaatggca
     6481 tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc tccggcaaag
     6541 cggatctctc cgctgtcatg ttccaccagg cggttaatca ttttcaaggt ggtggatttg
     6601 ccggagccag atgtgccaat cagcaccgaa aaactccctt cctgaaaatt gagattaaga
     6661 tcgttaacgg ctttttgtgc gccaaacagt ttgctgacat ggctaaattc aatcattacg
     6721 tttcaccttc agcagtgcga taagcaaatc gaacagggca tcgatcagca ccgccagaac
     6781 aattaccggg atcaccccca gcaacactaa atcaatggcg ctgcttagca gcccctggaa
     6841 aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca tacctacagt
     6901 ttgcaccatc accacccgca ggctgcgcag aaataccggt aacgccagtg gtaactgaac
     6961 atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca gcacatcgcg
     7021 cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg catagagcac
     7081 cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcttcc ccagccacgg
     7141 aaaggccgtc accagcgcgg caagcggcgc aatcaacagg ccaaagagcg ccaccgaagg
     7201 cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag tggaaaagta
     7261 gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac caaacagcaa
     7321 cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt attctttcat
     7381 tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa tggcaatctg
     7441 catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg catcgctaca
     7501 ggccagcaat gccagcgccg cagccagcca gaaaccactg ccgaggctgg tacgcgccag
     7561 cgcactgcca ttttgcgcca gttgtgtcgc cgcctttccc gcgccccaca ccagcaatac
     7621 gaagacgaat tgcgccagaa tgagtgcaca aatgctgcct tttttaccgg gaataaaaca
     7681 ggccgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg gccacagttg
     7741 ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtagctga taaacggcag
     7801 cgctgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa tacgtagata
     7861 agtcacggga aattacttcg cccacccttt ttgtttcagg tagtcggcag ccactttttt
     7921 ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt ttgcatcgag
     7981 gctggcgaag actggctgta gccactgtgc catttgcgga tactccttca acaccgattc
     8041 acgcaccact ggtgcaggcg catagatagg ttgcacgcct tgcggatcgc ttaaggtttg
     8101 cagccccagc gccgctaccg agccgtcagt gccgtaagcc attgcagcat taacgccaga
     8161 ggtttgctgg gcagcggctt tgatcgtcac cgccgtgtcg ccaccagcca gtgacagcaa
     8221 ctgatcctga ccgagcttaa agccataggc tttttcaaac gcgggtaacg catcggcgcg
     8281 ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctcttgca gataacgact
     8341 caggtcggca agcgaagtga gtttattttt ctctgccaca tcctgacgca cggcgatagt
     8401 ccaggtgtta tttgcaggcg cgggcgtcag ccagattaac ttgttgtgct ccgaatcgag
     8461 ctttttgact ttctcgtaac cttgctgcgc gtttttccac gctgcatcgt tttcatcttt
     8521 aaagaagaaa gcgccattgc cggtatattc cggatagata tccagttcac cggaagtaat
     8581 cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg gtacgccgtg
     8641 gctttccagc acctgcaaaa tgatattgcc gagtagcgca ccttcggtat cgatttttga
     8701 accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca acataaccag
     8761 tgaacctgcc cagagcttta agagtggcat gatgctttcc tcattctttt actgttgttt
     8821 tcagcgaatt aagagaaaag catagttgat aatggcgggg ttagcttgag cggaaggatt
     8881 cagttgcaga atcagataaa tacttaagag gcatattcgg tccggcgttt cctgccggat
     8941 gcggcgcgag cgccttatcc ggcctacaaa gggcgcaaac gtcgcgccct tactaaagca
     9001 ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa tcagtgccga taaatacatt
     9061 gaacttgcca ggctcggcgt catatttcat ctgttgattc cagaacttca gcgcctcaat
     9121 atcgatcggg aagctgacag tctgagtttc gcccggtttc agggtgattt tctcaaagcc
     9181 tttcagctgt ttcacagggc gactcatgga agccgtcaca tcctgcaagt acatctgcac
     9241 taccgtggcc ccctcgcgct taccggtgtt cgtcacctgc acgctggcag tcactttgcc
     9301 gtcacgcttc atggtcggcg cagaaagttt cacatcagag acggtgaaag tggtgtagct
     9361 cagcccatag ccgaacggat acaacgcccc gttagcttca tcaaaataac gcgaagtgta
     9421 tttgttcggc ttgtcggcat tatacgggcg accggtattc agatggctgt agtacaccgg
     9481 gatctgcccg acagaacgcg ggaaggacat tggcagcttg ccggacgggt tgtaatcgcc
     9541 aaacaataca tcggcaattg cattaccgcc ttcagtcccc gcaaaccagg tttccagaat
     9601 cgcatcagcc tgctgatctt ctttcaccag cgccagcgga cgcccgttca tcagcaccag
     9661 caccagcggt ttaccggtgg ctttcagcgc cgcaatcaag tcacgttggc tttgcggaat
     9721 agtgatatcg gtccggctgg aggcttcgtg cgccatcccc tgtgcttcac cgactacagc
     9781 caccaccaca tcagattgtt tcgccgtctg caccgcttca tcaatcatct cttgcggcga
     9841 acgcggatcg actttgaccg cttcttcata ctgattcagg aaatcgataa tgcctttgtc
     9901 actggtaacg ttcgcccctt tggcatacag cactttaccg ttttcaccga cggaattttt
     9961 aatcccggtc agtacggtca cggattgatc ggcaacaccg gctgcggacc agctgcccat
    10021 cacgtcacgt ttactgtccg ccagtggccc aaccaccgca atggtggccg attttttcag
    10081 cggtaacgtt tcgagacggt ttttcagcaa caccaagctt tcgcgcgcca cttcacgcgc
    10141 ttctttacgg tgcaggcggc tttcggcatt ggtatccacc gggtcagact ctttcggccc
    10201 caaatggctg tatgggtcgt taaacaatcc catatcatat ttaacgttca gtacatggcg
    10261 ggcagcatcg tccagctctt ccatcgtcac tttgccggat ttgatcaacc caggcagata
    10321 cttcgagtaa tactcgtcgc tcatactcat gttgattccg gatttcagcg ccacgcgcac
    10381 cgcatcttcc gggtctgccg ccgtgccatg tttaatcagc tctttgattg caccgtgatc
    10441 ggaaacggtg atgcctttaa agccccactg gtcgcgcaga acatctttca gcagccagga
    10501 atcggaggtg gctggcgtgc cgttcagcga gttcagcgcc accatcaccg cgccgctgcc
    10561 tgcgtccagc cccgctttgt acggcggcat ataatcatta aacaggcgct gcggactcat
    10621 atcgacggtg ttgtactctt taccgccttc taccgcgccg tatgcggcaa agtgtttgac
    10681 gctggtcatc accgagtagc gatctgccgg gcttttaccc tgcatcgctt ccaccatggt
    10741 tttacccatt gttgaggtga gatacgtatc ttcgccaaaa ccttcggaag cacgtcccca
    10801 gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc atattcaggc catcatctgc
    10861 cgcttcataa gcagagacac gtccgaccgt tttcactgca tcgaggttaa aagacgaggc
    10921 cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc agcacgtcgt aagcaaagaa
    10981 aagaggaatt ttcaggcggc ttaattccat cacctgatcc tgcatggcgc ggatatcctg
    11041 acgggttacg gtgttgaaaa tcgccccaac ctgaccgtct ttgatcatct cgcggatcgc
    11101 ctctttcggg ttatccgggc cgacgctgat taagcgcagc tgaccaattt tctcatcaac
    11161 tgtcattttc ttaagcagtt cggtgacgaa cgcatcccgc gcttcgggcg ttaatggatg
    11221 gttgccgaat aaatcatccg ccagtgctgg ctgcagggcc agactcaccg cgattcctac
    11281 tgaacatagc catttcatat ggatttttct tcctctgttg cccgacgagg cagcgaaaat
    11341 ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga gaaaaagaat gctcagttta
    11401 ttgtctgaat tttcaaaata ttcactcgct gaattgtcat acaaggcgct attctagttt
    11461 gtgatatttt ttcgccacca caaggagtgg aaaatgtctt ccatgacaac aactgataat
    11521 aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt cacacctgct caccgatccc
    11581 gcaaaaacgg cccgctatcg caagggcttc cgttctggtc agggcgacgc gctggctgtc
    11641 gttttccctg gctcactact ggaattgtgg cgggtgctga aagcctgtgt caccgccgac
    11701 aaaattattc tgatgcaggc tgccaataca ggcctgaccg aaggatcgac gccaaacggt
    11761 aacgactatg atcgcgatat cgtgatcatc agcaccctgc gtctcgacaa gctgcacgtt
    11821 cttggcaagg gcgaacaggt gctggcctat ccgggcacca cgctctattc gctggaaaaa
    11881 gccctcaaac cgctgggacg cgaaccgcac tcggtgattg gatcgtcgtg tataggcgca
    11941 tcggttatcg gcggtatttg taacaactcc ggcggctcgc tggtgcaacg tggcccggcg
    12001 tataccgaaa tgtcgttatt cgcgcgtata aatgaagacg gcaaactgac gctggtgaac
    12061 catctgggga ttgatctggg cgaaacgccg gagcagatcc ttagcaagct ggatgatgat
    12121 cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg cccacgatta tgactatgtc
    12181 caccgcgttc gtgatattga agccgacacg cccgcacgtt ataacgccga tccggatcgg
    12241 ttatttgaat cttctggttg cgccgggaag ctggccgtct ttgcggtacg tctagatacc
    12301 ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca ccaaccagcc ggaagtgctg
    12361 accgaaatcc gccgtcatat tctggctaac ttcgaaaatc tgccggttgc cggggaatat
    12421 atgcaccggg atatctacga tattgcggaa aaatacggca aagacacctt cctgatgatt
    12481 gataagttag gcaccgacaa gatgccgttc ttctttaatc tcaagggacg caccgatgcg
    12541 atgctggaga aagtgaaatt cttccgtccg cattttactg accgtgcgat gcaaaaattc
    12601 ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa actggcgcga taaatacgag
    12661 catcatctgc tgttaaaaat ggcgggcgat ggcgtgggcg aagccaaatc gtggttggtg
    12721 gattatttca aacaggccga aggcgatttc tttgtctgta cgccggagga aggcagcaaa
    12781 gcgtttttac accgtttcgc cgctgcgggc gcagcaattc gttatcaggc ggtgcattcc
    12841 gatgaagtcg aagacattct ggcgttggat atcgctctgc ggcgtaacga caccgagtgg
    12901 tatgagcatt taccgccgga gatcgacagc cagctggtgc acaagctcta ttacggccat
    12961 tttatgtgct atgtcttcca tcaggattac atagtgaaaa aaggcgtgga tgtgcatgcg
    13021 ttaaaagaac agatgctgga actgctacag cagcgcggcg cgcaataccc tgccgagcat
    13081 aacgtcggtc atttgtataa agcaccggag acgttgcaga agttctatcg cgagaacgat
    13141 ccgaccaaca gcatgaatcc ggggatcggt aaaaccagta aacggaaaaa ctggcaggaa
    13201 gtggagtaaa aattacggat ggcagagtat cgccatccga attcacttaa tcgttctgtg
    13261 ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt tttatagctc aacgctgctg
    13321 ccggcacagg catcacttta ccggtttcaa tccaggtacg caggcggctg gcatcggcaa
    13381 aatgggtata tttgccaaac gcgtccatca ctaccagcgc caccggttta ttattgataa
    13441 ccgtacgcat caccagacaa tggcccgccg cattggtaaa gccggttttg gttaactgaa
    13501 tattccagtt atcgcgatac accagatgat tagtattgcg gaacggcagc gtatacgtcg
    13561 ggttagagaa ggttgccata tcttcccggg tagtacttaa ctgcccgatc aacggatact
    13621 gtttgctggc aatgagcagt ttggttaagt cacgggcagt tgaaacgtta tgcaccgaca
    13681 atccggtagg ttcaacaaag cgcgtgttgt tcattccgag cgatttcgct ttcgcattca
    13741 ttgccttaat aaaggctttg taaccaccgg gataatggtg cgcaaggctt gccgccgcgc
    13801 ggttttctga agacatcagc gccagcaaca gcatatcttt acggctgatt tcgctattca
    13861 gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg gctgatatcc acttttagtt
    13921 tttcatccag cggcagtcgt gcatccagca caaccatcgc ggtcattaat ttgctgatag
    13981 acgcaatcgg acgcaccaga tccgggtggt tcgaatagat cactttgttg gtattcagat
    14041 caacaatcat cgcgctaccg gaggcaattt ccggttgtga agcggtggta gcggctgccg
    14101 ttttcgcaac ggcctgcggt gccaaaggca cagccagcat cagggccagg ctaaataaag
    14161 aaactcgaaa tttcggcatg atgagcattc agatagtggt tcacgcgcac gggttgcgca
    14221 ccgccggagt aaggatttac tgaggctagc gacgccatca taacgagcaa aaagtgcgat
    14281 cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca tttacgccag caatgcaatg
    14341 tcaaagaact ttctagaaca ggcgataccc gtagccccat agtataacgg ttagggcgag
    14401 cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa ctggagaagc taaggccttc
    14461 ctctttgttg atattcagga agctcggaat accaaggtaa agcaggtagc cggtgtaaaa
    14521 cagcgccacc gtgccgacca gcgcacacaa ccagaccagc ggataaagcg ccaccagacc
    14581 gcttaaaaac agcggagttg caacgtagcc cgcgaagacc atgcagtgcg caagtgacgg
    14641 acgctgcggg taattgcgcg ccatccacca gatgacccgc cccatcaccg caaccccagc
    14701 gagcataacg ccataaaaca ggacagccag cgccagtccg gtaaaccagg acaacttcag
    14761 gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg ccaatgaagg cgcaaatcac
    14821 cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg tgagaaatcg tttcgttttc
    14881 gcgattaatc acctgcattt cacgatcggg atgggaaaac agtccccaga catggctcat
    14941 accgccccct tgttgtgagt tcatgaacct gacagttcaa gtataagtca gcttgtgatt
    15001 attttttgtt cgctcatgta atttgcaccg tctgatgtac gtcctttttc agcgtgaatg
    15061 atttccaggg tgtatgctta caggcaacca aagggagaca gactggccta tggatctcaa
    15121 tacactcatc tcacaatatg gttatgccgc gctggtgatc ggtagcctgg cggaaggtga
    15181 aaccgtgact ttgctgggag gcgttgcggc gcatcagggg ctattaaagt tcccgctggt
    15241 ggtactttct gtggcgcttg gcggcatgat tggcgaccag gtgctttatc tgtgcgggcg
    15301 gcggtttggc ggcaagctgt tacgccgttt ctcgaaacat caggataaaa ttgagcgggc
    15361 gcagaaactt atccaacgcc atccgtatct gtttgtcatt ggtacgcgct ttatgtatgg
    15421 ctttcgggtg attggcccga cgctgattgg tgccagccag ctgccgccga aaatctttct
    15481 gccgcttaat attctcggcg catttgcctg ggcgttgatt tttaccacta tcggttacgc
    15541 tggtggtcag gtgattgcgc cgtggttgca caatctcgat cagcatttga agcactgggt
    15601 ctggttgatt ctggtcgtgg ttctggtggt aggcgtgcgc tggtggctga aacgacgcgg
    15661 gaagaaaaag ccggataatc aggcgtaaaa ccattgccct ggataaggcg ttcgcgccgc
    15721 atccgacatc cggtgcacca tgcctgatgc ggcgcttgtg cgtcttatca tgcctaccaa
    15781 aattttaccc attgaaatat ccacacacct cactgcgcta ttctgggttg aactgcggat
    15841 tcgccaacat aaagccgcca tccactatca acgactgccc ggtggtgtaa tttgcgccct
    15901 ccgaacaaag ccacgccacc aggctggcaa tctcatacgt tgcgccaaaa cgccgcagag
    15961 gaatcgaagg ctccgcgtcg ggcttcacgt cgctatcatc catgccgttc attggcgtag
    16021 cgatcgcccc aggtgcgact gcgttcacca aaatcttatg cctgaccaac tccagcgcca
    16081 ttgctttggt taacccgccg agcgcatgtt tagcggctgt gtaggcgctg gcatccggca
    16141 gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg accaccctgc ccttgtttca
    16201 ccatctggcg ggccgcaatt tgtgagcata aaaatgcacc atcgacatca acggtaaaaa
    16261 tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcgc tttggtcatt gcgcccgcat
    16321 tattcaccag cacatcaata cgccccagcc gttgaatgag tttctccagc gcctgtgcac
    16381 cttctggcag tttaccgaga tccagctgca caatctccgc acgtacgccg tggctaacta
    16441 cctcacgcgc ggtatctttt gccccttctt catctgagtg ccaggtaatg ccaatatcaa
    16501 acccctgctg cgccagtaat aacgcgcact ctttgccgat ccccgaatcg gaggcggtaa
    16561 taatcgcaac ctgtgccatc gagttctcca cttaacgctg aataaacgtt aagtatagaa
    16621 ggcgcatatc atcagcgttt gtaacccccg cccaacgcac cagtgagttg aatggaagca
    16681 tccagccact gcccttgcaa taacaggcca ttggcccgct cacgcagcgc ggggattctg
    16741 gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa agcgcgcctg cgcaagacct
    16801 accacacgca aggcatcgcg ctcaatttgc gcctgatgct ggtttttctc cgcaagtgtc
    16861 tgaacctgac tggctgcccg cgccacgtca ttcaccgctt caaccaccgc tttgttgtag
    16921 ctggcgatag acaagttgct ttcggctttt gcgatatcga gattggcgtt aagacgacca
    16981 ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca tttgctgcgc ggaatgacgg
    17041 aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg ccatcaggtt gatgtcagga
    17101 taaaatgccg cttttgccgc atcaatggtg cttagcgatg actcaacgta ccagtgcgcc
    17161 gcctgcaaat ctgcccgccg ggccagtaag gagtacccca gttcatcagg aagctggctt
    17221 gccactttcg gcaacgcgac cggtttaagc ttcaatgact ttgtctggtt atttgtaagt
    17281 gcgcttaacc gtgcctcaat aattttcatt ttccccgcga catcgttgag ctgctgccgg
    17341 gttttgctgg cattaatatc ggtttccaca ccttcaactg aagaagtaat cccgttctga
    17401 tatagctggc gatcggtcgc gataatggtg ttctgctctt tttctatttg ctgcaagacc
    17461 gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc gggctacgct gccagccagc
    17521 aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa ccgtacccag gcgggcagta
    17581 acctccgccc gattctttcc ccagatatcg agatgccagc ccgccgttaa gccaaaagtt
    17641 ccgttggtgt accacggacc ggtcgtacct gcggccggat cgttcagagc aaacggcccc
    17701 attaagcctt ctgccgacat tttttgccgc tccatatccg cagaaaagtc gatctgcgga
    17761 ccatcctgag tagcaattgc cttcgcctgg gcttcagcta gctgaatgcg ctgttcagcc
    17821 acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta aggaagtgag ttgattatcg
    17881 tgatactcca gccaccattg gctgtctggc caaccatttt tcagcgccgt gggtaatgcg
    17941 gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct ggcgggtttc atgcataggc
    18001 gcacacccgg ccagcatcag taacagcgga aaacaggcga tggcgggata aaaggaatca
    18061 cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg agattaccgt gtgttgcgat
    18121 atatttttta gtttcgcgtg gcaatacatc agtagcaata aaacgacata tccagaaaaa
    18181 tatacactaa gtgaatgata tcttccgatt tatcttaatc gtttatggat aacggcaaag
    18241 ggcttcgttt tttcctatac ttattcagca ctcacaaata aaggaacgcc aatgaaaatt
    18301 atactctggg ctgtattgat tattttcctg attgggctac tggtggtgac tggcgtattt
    18361 aagatgatat tttaaaaatc agatatggca tataagttgt caggtcagga actagatatt
    18421 ttgtgtactt gatttgcaaa cagctccggt gttggtggcg tcaaaatcgc tgaagcgttc
    18481 cccgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgtcttac aaggacgtta
    18541 cctcgcgccc gacccggtag acgaagggga taagctgaag gtaccgcgca gcggcgattt
    18601 tgttcgccag agcccggggg tgcaggggga ggaggcgatt gtcctccccc tgcgcgctcc
    18661 ttgcgccagt ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga tgatgaagct
    18721 gaataagcga ttcattgtct ctgataaact gcgattccac tattatcgca gcttatcgat
    18781 atcaattgcc tgaatagccc ttgcaatatc aggggaatta ttcaacaccc gaacatgctg
    18841 aaataattcc gttgcttcat cgtattcttt acgcaaataa ctcaaccact gtttaatccg
    18901 cgcaacgtga tataacccgg tatcgccctg cttttccaga cgggtatatt tttgcagcaa
    18961 agcaaccacc tccggccacg gcattcgcgg ttcgttatat tttactaccc ggctcaggtt
    19021 gggaatattg agcgccccgc gaccaatcat cactgcgtcg cagccgctga tcgcaatgca
    19081 ttgttgcgcg ctctgccagt cccagatttc gccgttggca atcaccggaa tattcagccg
    19141 ctggcgaatc tcgccaatcg cctgccagtc aatatgctcc gcgcggtaac cctgctcttt
    19201 tgtccgtcca tgcaccacca gctccgtagc accagcctgt tgaaccgcat cggcgatttc
    19261 aaatttcttc tcaccgctgt cccagcccag acgcactttc acgctgacgg gcaaatgcgc
    19321 cggtacagct tcacgcatcg cttttgcacc ctggtagatg agttcaggat ctttgagtaa
    19381 cgtcgcccca ccaccgctac cgttgaccgt tttcgacggg cagccgcaat tgagatccac
    19441 gccccaggaa cctaattcca ccgcacgggc ggcgttctct gccagccatt gtgggaactg
    19501 acccaacaac tgcacgcgca ccagcgtacc ggatggtgtc cggctggcgt tttgtagctc
    19561 agggcaaatg cgatgaaaaa cttttaccgg cagcagttga tccaccacgc ggacaaactc
    19621 ggtgatgcac agatcgtagt cgttaacttc ggtcagcaat tcacgcacca gagagtcaag
    19681 cacaccctcc atcggagcca gtaacacacg catatcttca cccgcaaaaa aatgaggcgc
    19741 tatgttagcg cctatggtca acggattaaa ggtctgcaaa gagggattgc tgcaaactgt
    19801 aatccctctc tatgcttgat gtatgcctga gttcgttgcg cattgcgtaa tgccggggag
    19861 cgaaaaaata agcgctgatt tcttaatgtg atcggtagca cgttttacga attaattgta
    19921 tgatgaatcc atctcatctg gggtgttgat tatgagtaag acactgaaca ttatctggca
    19981 atatttacgc gctttcgtcc tgatttatgc ctgcctgtat gcaggcattt tcattgcttc
    20041 cctgctaccg gtaaccattc cgggcagcat catcggtatg ctgatcctgt ttgtcctgct
    20101 ggccttgcaa attcttccgg caaaatgggt caatccgggg tgctacgtac tgattcgcta
    20161 tatggcgcta ttgtttgtgc cgattggcgt aggcgtcatg caatattttg atttgctccg
    20221 cgcacagttt ggcccggtag tggtttcctg tgcaatcagt acgctggtgg tttttctggt
    20281 ggtgagctgg agttcgcaac tggtacacgg tgaacgtaaa gtcgtaggtc agaaaggatc
    20341 agaagaatga tggcaaatat ctggtggtca ttaccgctga cgttgattgt cttttttgcc
    20401 gcccgcaaac tggcggcacg gtataagttt cccctgctta acccgttgct ggtagcaatg
    20461 gtggtgatca ttcctttttt aatgctgacc ggcatctctt acgacagcta ctttaaaggt
    20521 agcgaagtgc taaacgatct gctgcaaccg gcggtcgtcg cgctggccta tcctttatat
    20581 gagcagctac accagatccg cgcgcgctgg aaatcgatca tcaccatctg ttttatcggc
    20641 agcgtggttg cgatggtgac ggggacttcc gtggcattgt tgatgggcgc ttcaccggaa
    20701 atcgccgcgt caatcctgcc gaaatcagtc accacgccta ttgcaatggc ggttggcggc
    20761 agtattggcg gtattccggc aatcagcgca gtttgcgtga ttttcgtcgg catcctcggc
    20821 gcggtatttg gccatacatt gcttaatgcg atgcgtattc gtaccaaagc tgcgcgcggt
    20881 ctggcaatgg ggactgcctc gcacgccctc ggtacggcgc gctgcgccga gctggattat
    20941 caggaaggtg catttagttc gctagcgctg gtgttatgcg ggataattac ttcgctgatc
    21001 gcaccgttcc ttttcccgat tattctggca gtaatgggct aaaatttgcg atgcgtcgcg
    21061 catttttgat gtaagtttca cgcgttgcat aattaatgag attcagatca catataaagc
    21121 cataacgggt tcgtaaactg ttatcccatt acatgattat gaggcaacgc catgcatcca
    21181 cgttttcaaa ccgcttttgc ccaacttgcg gataacttgc aatctgcact ggaacctatt
    21241 ctggcagaca agtacttccc cgctttgttg accggggagc aagtctcatc gctgaagagc
    21301 gcaacggggc tggacgaaga cgcgctggca ttcgcactac ttccgctggc ggcggcctgt
    21361 gcgcgtacgc cattgtcgaa ttttaatgtt ggcgcaattg cgcgcggtgt gagcggaacc
    21421 tggtatttcg gtgccaatat ggaatttatt ggtgcgacaa tgcagcaaac cgttcatgcc
    21481 gaacaaagcg cgatcagcca cgcctggttg agtggtgaaa aagcgcttgc agccatcacc
    21541 gttaactaca cgccttgtgg tcactgccgt cagtttatga atgaactgaa cagcggtctg
    21601 gatctgcgta ttcatctgcc gggccgcgag gcacacgcgc tgcgtgacta tctgccggat
    21661 gcctttgggc cgaaagatct ggagattaaa acgctgctga tggacgagca ggatcacggc
    21721 tatgcgctga ctggtgatgc gctttctcag gcggcgattg cagcggcaaa ccgttcgcac
    21781 atgccttaca gtaagtcgcc aagcggtgtc gcgctggaat gtaaagacgg tcgtattttc
    21841 agtggcagct acgctgaaaa cgccgcattc aacccgactc tgccaccgtt gcagggagcg
    21901 ttaattctgt tgaatctcaa gggttatgat tacccggata tccagcgcgc ggttctggca
    21961 gaaaaagccg atgcgccgtt gattcagtgg gatgccacct ccgcaacgct gaaagctctc
    22021 ggctgtcaca gtatcgaccg agtgcttctc gcttaagcct ggtgccggat gcggcgtgaa
    22081 cgccttgtcc ggcttgccag ccctctcctg gtgtcgaaat tcccggcaaa cagtttgccg
    22141 tttcttgcgc aaaaccagcg ggtaaagtag cctgatggaa attttcctta gatcgagtct
    22201 cctgcatgtt aaagcgcgtg ttcctcagcc tgttagtcct gatcggcttg ctgctgttga
    22261 ctgtgctcgg cctcgatcgc tggatgagct ggaaaaccgc gccttatatc tacgacgaat
    22321 tgcaggatct cccctaccgc caggtcggtg tggtgctcgg aacagcaaaa tattatcgta
    22381 ctggcgtaat taatcagtat tatcgctacc gcattcaagg agcgattaat gcctataaca
    22441 gcggtaaggt aaattatcta ttactgagcg gcgataacgc attgcaaagt tataatgagc
    22501 cgatgaccat gcgcaaagat ttaatcgctg ctggtgtcga cccatcagat attgttctcg
    22561 attacgcagg ctttcgtacg ctggattcca tcgtgcgtac acgcaaagtt ttcgatacta
    22621 atgatttcat tattatcacc caacgtttcc actgtgagcg ggcattattt attgcgctgc
    22681 atatggggat tcaggctcag tgttatgccg taccgtcacc gaaagatatg ctgtcagtac
    22741 gtattcgcga atttgccgcc cgtttcggtg cgctggctga cctttatatt tttaaacgtg
    22801 aaccgcgttt tttagggccg ctggtcccta ttccggctat gcaccaggtg ccggaagatg
    22861 cgcaggggta tcccgccgtc acacccgaac agttacttga attacaaaag aaacaaggaa
    22921 agtagttatg gatgtacagc agttttttgt cgttgccgtt tttttcctta ttccgatatt
    22981 ttgtttccgc gaagcatgga aaggctggcg cgcaggcgcg attgataaac gggttaaaaa
    23041 tgcaccggaa ccggtgtatg tctggcgagc aaaaaatccc ggactctttt tcgcttatat
    23101 ggtggcatat atcggcttcg gaattttatc tatcggcatg attgtttatc ttattttcta
    23161 tcgttaatat tt
//
LOCUS       DAZFKM010000076        22657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFKM010000076 DAZFKM010000000
VERSION     DAZFKM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            321..1220
                     /gene="yegS"
                     /locus_tag="ACHKAY_003040"
     CDS             321..1220
                     /gene="yegS"
                     /locus_tag="ACHKAY_003040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO5701686.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1302..2075)
                     /locus_tag="ACHKAY_003041"
     CDS             complement(1302..2075)
                     /locus_tag="ACHKAY_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5701687.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2181..3221)
                     /gene="gatD"
                     /locus_tag="ACHKAY_003042"
                     /pseudo
     CDS             complement(2181..3221)
                     /gene="gatD"
                     /locus_tag="ACHKAY_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            complement(3269..4624)
                     /locus_tag="ACHKAY_003043"
     CDS             complement(3269..4624)
                     /locus_tag="ACHKAY_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO5701688.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4628..4912)
                     /gene="gatB"
                     /locus_tag="ACHKAY_003044"
     CDS             complement(4628..4912)
                     /gene="gatB"
                     /locus_tag="ACHKAY_003044"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO5701689.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4943..5395)
                     /gene="gatA"
                     /locus_tag="ACHKAY_003045"
     CDS             complement(4943..5395)
                     /gene="gatA"
                     /locus_tag="ACHKAY_003045"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO5701690.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5405..6667)
                     /gene="gatZ"
                     /locus_tag="ACHKAY_003046"
     CDS             complement(5405..6667)
                     /gene="gatZ"
                     /locus_tag="ACHKAY_003046"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO5701691.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6696..7550)
                     /gene="gatY"
                     /locus_tag="ACHKAY_003047"
     CDS             complement(6696..7550)
                     /gene="gatY"
                     /locus_tag="ACHKAY_003047"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO5701692.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7860..8912)
                     /gene="fbaB"
                     /locus_tag="ACHKAY_003048"
     CDS             complement(7860..8912)
                     /gene="fbaB"
                     /locus_tag="ACHKAY_003048"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO5701693.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9169..10446
                     /gene="yegT"
                     /locus_tag="ACHKAY_003049"
     CDS             9169..10446
                     /gene="yegT"
                     /locus_tag="ACHKAY_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO5701694.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10443..11447
                     /gene="yegU"
                     /locus_tag="ACHKAY_003050"
     CDS             10443..11447
                     /gene="yegU"
                     /locus_tag="ACHKAY_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO5701695.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11444..12409
                     /gene="yegV"
                     /locus_tag="ACHKAY_003051"
     CDS             11444..12409
                     /gene="yegV"
                     /locus_tag="ACHKAY_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO5701696.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12383..13129)
                     /gene="yegW"
                     /locus_tag="ACHKAY_003052"
     CDS             complement(12383..13129)
                     /gene="yegW"
                     /locus_tag="ACHKAY_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO5701697.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13181..13999)
                     /gene="yegX"
                     /locus_tag="ACHKAY_003053"
     CDS             complement(13181..13999)
                     /gene="yegX"
                     /locus_tag="ACHKAY_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO5701698.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(14064..14864)
                     /gene="thiD"
                     /locus_tag="ACHKAY_003054"
     CDS             complement(14064..14864)
                     /gene="thiD"
                     /locus_tag="ACHKAY_003054"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO5701699.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14861..15649)
                     /gene="thiM"
                     /locus_tag="ACHKAY_003055"
     CDS             complement(14861..15649)
                     /gene="thiM"
                     /locus_tag="ACHKAY_003055"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO5701700.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15684..15780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15872..16144)
                     /gene="rcnR"
                     /locus_tag="ACHKAY_003056"
     CDS             complement(15872..16144)
                     /gene="rcnR"
                     /locus_tag="ACHKAY_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO5701701.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16265..17089
                     /gene="rcnA"
                     /locus_tag="ACHKAY_003057"
     CDS             16265..17089
                     /gene="rcnA"
                     /locus_tag="ACHKAY_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO5701702.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17308..17646
                     /gene="rcnB"
                     /locus_tag="ACHKAY_003058"
     CDS             17308..17646
                     /gene="rcnB"
                     /locus_tag="ACHKAY_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO5701703.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17728..18762)
                     /gene="yehA"
                     /locus_tag="ACHKAY_003059"
     CDS             complement(17728..18762)
                     /gene="yehA"
                     /locus_tag="ACHKAY_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO5701704.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18776..21256)
                     /gene="yehB"
                     /locus_tag="ACHKAY_003060"
     CDS             complement(18776..21256)
                     /gene="yehB"
                     /locus_tag="ACHKAY_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO5701705.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21272..21946)
                     /gene="yehC"
                     /locus_tag="ACHKAY_003061"
     CDS             complement(21272..21946)
                     /gene="yehC"
                     /locus_tag="ACHKAY_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5701706.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(22027..22569)
                     /gene="yehD"
                     /locus_tag="ACHKAY_003062"
     CDS             complement(22027..22569)
                     /gene="yehD"
                     /locus_tag="ACHKAY_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5701707.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         5729 a         5740 c         5224 g         5964 t
ORIGIN      
        1 tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt cagggtaact
       61 ccatgggggc tttaatatat ttggaatagt atatttaaga tgccactgtt tggttgaata
      121 ttaggcatat gctctttttt gaaatttatc ggtggcagcc gttagtattc gctgtcccca
      181 ttgcaagctc ctggtggtaa ccactgaatc ctccatactt gaactgactt tttatcctcc
      241 gactttcatc ctgttctgac tccacctttt gttttctgct ctacactatc tacagaccaa
      301 tcataaaggc acatacgatc atggcagaat ttcccgccag cttactgatt cttaatggca
      361 aaagtactga caatctaccc ttgcgcgaag caattatgct gttgcgtgag gaaggaatga
      421 cgatccatgt gcgggtcacc tgggagaaag gcgatgccgc acgatatgta gaggaggccc
      481 ggaagttggg cgtcgcaacg gtgattgccg gtggtggcga tggcaccatt aatgaagttt
      541 ctacggcgtt gattcagtgt gagggggatg acatacccgc gctgggaatt ttgccattag
      601 gaaccgccaa tgattttgcc accagtgtag ggattcctga ggcactggat aaggcgctga
      661 aactggcaat tgccggtaac gccattgcga tagatatggc gcaggtcaac aaacaaacct
      721 gttttattaa tatggcgaca ggcggatttg ggacgcgtat taccacagaa acgccggaaa
      781 aattaaaagc cgcgctgggt ggcgtctctt acatcattca tggcttaatg cgcatggata
      841 ctctgcaacc ggaccgttgt gaaatccgcg gtgaaaactt tcactggcaa ggtgacgccc
      901 tggtcattgg tattggtaac gggcgtcagg ccggtggcgg tcagcaattg tgtccgaacg
      961 cgttaattaa cgatggcttg ctgcaactgc gcatttttac cggcgatgaa attcttccgg
     1021 ctctcgtatc aaccttaaaa tctgacgaag ataacccgaa tattatcgaa ggcgcttcgt
     1081 cgtggtttga tatacaagcc ccacacgaaa tcacttttaa tcttgatggc gaaccgttga
     1141 gcggacaaaa ctttcatatt gaaatacttc cggcggcgtt gcgttgtcga ttaccaccag
     1201 attgtccatt attgcgttaa tcaaactatt ctctcttgaa ccagggagaa tccgaaatgc
     1261 cggatgcggt gtaaatacct tatccggcat acagaacaat actacaccgt aattaatttt
     1321 acccgtgtct tctgcaatgc cagtttatcc acctctgata cagcatcatc ggtaataata
     1381 atgtcaattt tttcgattgg cagtacctga ttaaaaccgc gacggttaaa tttcgatgaa
     1441 tcgagaacgg caatcacttt attagcggcg gtgaccatcg caccgctaat cgaataacct
     1501 tcattaaagg tcgtaatacc attaaccgca tcaataccat cagcaccgac aaacattaaa
     1561 tcggcattaa tatcctgcag ggaacgttca gcaatagatc catgcataga tcgcgtttta
     1621 tggcggaccg tgccaccgca gacgaccaga gtaatgtctt tattttcgga aagggcaaac
     1681 gcagccggga gactgttggt aatcaccgtg atatttttgg cggtcattaa tccttcggcg
     1741 ataagcattg tggtacttcc gctgtcgaga ataacggtca tcccttcatg gatcattttg
     1801 actgccgcct gcgctattct gtttttcggc gcgctggcga gctgaaatcg ctctttaaac
     1861 ccgacttcct gggtctcggt ttcactatta ccggacatta ttttcgccgc accgccatga
     1921 aagcgcgtaa ccacgccttt ttgttcgaga aagcgcaaat cggcacggat tgtcgcttcc
     1981 gaggcagcaa atactcccgc cagatcctga acaagcacgg ttccctgttc attcactaat
     2041 tggatgatct tattccttcg ctcgaatgaa ttcatcgtca tttacccata ttcaattgtg
     2101 gctagtgtaa acgaaggggg accattggtg aatgatctgt tttgcacaat accgggccag
     2161 catcacgctg gcccgcggtt tcagggaatg agcaacactt tgcccggcat agcattacga
     2221 gcgatgtcac gcaccgcctg ggcgaagctt tcaaagcttc cacggtgagc gattaatggc
     2281 tccaggctta acttacgttc tgtcagcaac cggctcgccg tttcccactc ctgccccggc
     2341 caagggctgg agtagttcat ccaactgccg ataaccgtca gctctttacg caatattttg
     2401 ccaaacgttg ccgatgttaa atgcagatcc tgatgcaacg tgcccaccag cgccagttgg
     2461 gcatgaggac cggcaatctc taccgccagt tcgacagttt gcggtacgcc agccgtctcg
     2521 aggataagct gattaaagcg cagttagcgt aaaacgctct gcatttgcgg cgcgctcatt
     2581 tcgctactgt taaatgtttg catcgcaccg aaagattttg ccagtgccag tttttctgaa
     2641 ctaatgtcga tcgccgtcac actctttgct cccagcgcga cagcgcactg aatggccagc
     2701 aggccaatgg ttccggcacc aataataata acgtttttat tctcacaacc ttgcgctaaa
     2761 tgaaaagcat gcaggccaac ggtaatcggt tcaataaaag ccccatcctc aataggcata
     2821 tcagtaggta gagcaaagac atttttccgc ttaacgacaa tatattcggc aaatccacca
     2881 tcacgccgtg agccaataaa atcatatttt gcgcactggg aataaaaccc tttcaaacac
     2941 tctggacaag taaaacaggg taataacggc acacaggcaa ccgcatcgcc aggatgtaaa
     3001 tcatcaacac cggatcccac cgcatcaata tagccgctaa attcatggcc taacgttatt
     3061 ggataataat gtgcaccatt tttaaatatc ctgggtaaat cggaaccaca taagcccgag
     3121 ctggcaattt ttacccgcac ctcatcctga tgtttaattt caggaatgac gctttctgca
     3181 acgcgcacga taccatcagt atcattcacc actgatttca taaaaactcc tgattgttaa
     3241 gggggataac ctccccctca ggtaaaaatt attctgcgag aacgactttc tcttgtttaa
     3301 taaagccacg cgctctacgc caggtcatga aaataccggt aagataaatt gcgccgataa
     3361 taatgaatcc gggaatattt tgcggggaga aaacctgaat cagtaaccag gtaatgggag
     3421 aaccgccctg atccattgaa gccaccatac ccccggcttt taacgcccca gcattagccg
     3481 ccagttgggt gtgcaaacca atagtttgcg tcgcgatcca cagagtgatg ctcatgatga
     3541 tgacacccga gattaaggtg cggaacagat ttccacgatg cacggcgact gccatcgcca
     3601 caaagaagcc gatggtggca agatcgccaa acggcagcac ctgattaccc ggaacacaaa
     3661 cagcaattaa aatggtgagc gggataaaaa tcaggcttgc cgataccacc gccgtatgcc
     3721 ccagcagtaa tgcgggatcc aggccaatca ggaactcctg accgccgaac ttcgcctgta
     3781 aacgactacg ggcctgctta gcgatgggcg ttaaaccatc catgatgggt ttaatcaccc
     3841 gtggcattag cagcatcact gccgccgttt ttaccgccag ctgcaataca cctttgacat
     3901 cgtaacccgc gaggatgccg ataatcagcc ccatcacaaa acccacggtg acaggctcgc
     3961 caaatggacc aaatttgcgc tgaatatcgt cggcgctaaa tttaattcgg ttaacgcctg
     4021 ggattttctc gatgatagca tcgaccagca ccgcaatcgg ccccatatac gccgacgtac
     4081 cgtgcggaat agcaatacct tccagctcaa agaaatttcg ggtatcgcgg gcaaaccagt
     4141 cgccgagctt ataaacaaac gccgcgtgaa ttaccacacc tgccatccct atcatccatg
     4201 aaccggttgc caggtgcagc aacgcgccgg tgaaggtcat atgccagata ttccagatat
     4261 caacatttac cacccgcgtc atacgggtca gtaacatcgc cacgttaacc aatatcgcaa
     4321 tcggaatcgc caccagcgca atttgcgacg cccaggtcat tggtgaagag cccggccagc
     4381 cgacatcgac cacatgcaga ttcaggtcga aattttccgc catcgctttc gccgccggac
     4441 caatggaatc cagcattaag ccaatcacaa ggccaatgcc aacaaagcca atcccgatat
     4501 gcagacccgc tttaaagcaa tcgcctgcct tcatgcctaa tattttagaa aaaataatga
     4561 tgacaatcgg aagcatcacc gtagggccga ggtcgagaat ataacgcatg acttctgaaa
     4621 acataggtca cccctgtaag atagtcagaa ttttattttg taatgcttcg ataccgacac
     4681 cagaaataaa aggcatgccg tgaactaacg gaatatcgcc aaaactacga tccactttgg
     4741 cagtggtgca tatcaaatgc acaccatcca tataggtttc tatttcatta acccgacact
     4801 ggattaattc aacaggaata ttatgattct gacacaactc tttaatttct tccgccgcca
     4861 tcgtagaggt cgcaaccgcg cctccgcaag cgacaataat cttgcgtttc ataaatagtc
     4921 ctttttattg ttacagaggg atttattcgt ctgaatctaa aacatacttt gtgaagtatt
     4981 cctttaactg agtttcagga agagtaatta gtgtctcgac gatatcgggc tgttgtaact
     5041 tgccaaataa acagcgtaaa agtttcaatt gctgctgcgg attttccaca atcaacgcaa
     5101 taaccaacga cacctccacg tcgttatcat catccgcttg ctgaaaatga actttatttg
     5161 ttggccttaa cagataaatg gctgacgact tagcatgaat cgcctcacaa tgcggtatag
     5221 caatggcgtg ctgctcaagc attatcccgg tagggaattc tgcttctctg gcaattaatg
     5281 cctgtggcca ggtatcataa accacacctt tcgcgagcat ctcattaccg atatgggtta
     5341 aaacttcgct acgatcgaca aaagaaattc cgctacgaac aaacaggtta gtcatatacc
     5401 gtccttattc cgcacagccg tagcgatagg cgcgcaaaac atcataaatt ttatccataa
     5461 tcagctgatg cggtattgct gataattccc cggactgaat gcgttcaaat tgtttgggaa
     5521 gatactgact aatcatgccc agtgggatgt ccacgccttc aagattcacc atcatcgttt
     5581 cgacgctatt tttaatccga ctatgcggcc agtaataacg aatacgatcc gacaggctgt
     5641 aacgaatatc cagtaatgaa tcgttaaaac ccgtacgata atattttttc cagtattgcg
     5701 gttcgtcgag catcacttct tcaattaccg ccaggcaacc gctgcgattt tcaggggcga
     5761 taagttcctg ctcaatttgt gccagtgcaa atatcgcctc gcgtaaagca aaggttaatg
     5821 cgggaccgac tttcaatatt gcaaagtgat cgcggactaa ttcccaataa gccgtccggg
     5881 tctggtaatc ggtagaatgt gcttcataaa ccattcgggt gctttctatc cattgcgcca
     5941 gcggctgcgc ttcctgcggc tgataatgga taatattgct gtgatcaaat tccacgcccg
     6001 gctgcaccac gatggcaatc acgcgtgtta acgcctctgt cagcccacgg gcaataaagg
     6061 ccttttgatg cgtacgtaaa gtattggcgg catcttcaac atgggtgatg tgtactgact
     6121 gaatggcgct ggcctcaccg cccggaaccg gaacttcggt gccaatgaca tagctcagtt
     6181 gctcacgctg gcaatctgtc gccacacttt ccgcagcaaa gcaaagcaca gcagctcgtt
     6241 ccgcaaccgt ttctggtgct aacggtatgg gatcccccgc gcaggacatt gacgcatcaa
     6301 gatgaatttt actgaaaccg gcacgaacat attcctttac cagctcgacg gatttttcca
     6361 tcgccgcatc cgcattttct tgctgccagc agtttggccc cagatgatcg ccgccgagaa
     6421 taatgcgttc gcgtgcaaac ccaactttat cggcaatcgt aaaaacaaat tcgcgaaagt
     6481 ctgccggtgt cattccggta taaccgccaa attgattgac ctggtttgac gttgcttcaa
     6541 tcagcacttt gcgcgtgctg ttgcgatcaa atgccagcgc cgcttcgata accaacggat
     6601 gggcagaaca gactgaacat atgccgatat gttcaccagc tttatgccgg gcaattaacg
     6661 ttttcatgtt ttttccttat taaatggcag gtgcgttatg ccctgccctc gcagccacaa
     6721 tcggcaatca ctttgctcac cacatcgcgc attgcggatt tagccgactg caaataatcc
     6781 cggggatcgg tcgcttcagg gtgctcggtc aggtaatttt ttaacgcctg cgagaaggca
     6841 tttttcagct ccgttgcaac gttgattttg catatcccca gtttgatggt ttgctgaata
     6901 tccttagtcg ataaccctga cgcgccatgc agcaccagcg gtaagttcac ccactggcga
     6961 atgttctcca gtcgagaaaa atcaagcgcc ggtgcgctgg catacatccc atgagccgtg
     7021 ccgatggcga ccgccaggga atcaattccg gttgcctcgg caaattcacg cgcctgagcg
     7081 gggttggtat aaaaagcatc ggcttcattg acttgcacat catcttcctg gccgccaagt
     7141 tgccccagct ccgcttcgac gctgacatca aagcgatggc aaaaatccac cacctctttg
     7201 acccgtgaaa tattttgcgc aaaaggcaaa tgcgaggcgt caatcatgac tgagcgcacg
     7261 ccagaacgaa ccttctgagc gatatcgtca aatttcgtgt gatggtcgag atgaattgcc
     7321 agtggatggt gatattgctt cgccatcgcg ctgaccagcg ccaacagatt ttctgtacca
     7381 gcatgagtaa atgtgccagg cgttccggcg atgatgaccg gcgcatgcag gttggcagcg
     7441 gtttccacca ccacttgcat cgtttcgaga ttgtgaatat tgaatgccgg aaccgcataa
     7501 ccgccgcgct gtgcgttgtt cagcatctgc tttgtcgata ccacgtacat tttcatatcc
     7561 tgtcgtttgt tttcgatttc aaaatataat gaaatgattt gtttttaaat gtcgagataa
     7621 cgatcacaaa agcgacaatg tgaaaattgt tcgaggagta aaaggcaaaa aaaaacggcc
     7681 tcccgatagg gaagccgtag caaagtgcgc gtgtttttat gccggatgcg gtgtaaacat
     7741 acgtggcagt gctatgtagg catgataaga cgcttccgcg tcgcatcagg cataggttgc
     7801 cggatgcagt gtaaacgcct tatccgtcct acggggtggt gctgttcgta tgaggcgtat
     7861 caggcaatag taattttgct atcgagataa acgtcctgca cggcattaat cagtttcacg
     7921 ccgtcagcca tcgatttctt gaacgcctta cgtccaagaa tcagccccat tccgccagcg
     7981 cgtttgttga taaccgcagt acgcactgca tcgctgaggt cagtttcacc gcccgcagca
     8041 ccgccggagt ttatcaaccc agcccgaccc atatagcagt tagctaactg ataacgcacc
     8101 agatcaatcg ggttttcgct ggtcaatttg ctgtaaacac gatcgtcggt gtaaccgtaa
     8161 ttaattgctt tatagccgcc gttattttcc gccatttttt gtttgacgat atctgcaccg
     8221 atggttgccg ccagatggtt tgcctgaccg gtcaggtcgg cggaaacatg gtaatcaacg
     8281 ccatctttct taaaggcgga gttacgcaaa taggcccaca gcactgtcac catacccagc
     8341 tcgtgcgcac gttcaaaagc cgcagaaatt tcttcaatct ggcgacgtga ctcttccgag
     8401 ccaaaataga tagtcgcacc aaccgcaacc gcgcccatgt tgaacgcctg ctccacgctg
     8461 gcatacagcg tttgatcgta ggtattcggg taacttagcg tctcgttgtg attaagtttg
     8521 acgaggaatg gaatgcgatg cgcataacgc cgcgataccg acgccagcac gccgtaagtt
     8581 gacgccacac agttacagcc cgcttcgatc gccagttcaa caatgttttt cgggtcaaag
     8641 tagagcgggt tagcagcaaa tgaagctccg gcagagtgct caacgccctg gtcaaccggc
     8701 agaatagaaa gatatcctgt gccagccaga cgcccggtgt tgtacaacgt ctgcatatta
     8761 cgtaacaccg ctggcgggcg attattgtca atcattacgc ggtctacgta gtcatgtccg
     8821 gggagataaa gctggtcaga aggaattgtc atacaacggt gctgtaaaag gttgtcggcg
     8881 tctttgccaa gcaactgcgc aatatctgtc attactatgc tcccgtaaat tccgattgga
     8941 tatcggctat ggattgtcct ggcccgcctt ttgcgggcaa tcataatcct ggtcgttacg
     9001 ggcgagtttt tccatctttt ggatgttttt tcagcgtttt ctgttggctc gattcatcag
     9061 aaaaaatgtt agcgcggtca agttttcacc gcaaaggtat ttaaaaggta ttattaagtg
     9121 gtattgtcat cgcgtacctt acattacctg tcatgaagga attaaaagat gaaaacaaca
     9181 gcaaagctgt cgttcatgat gtttgttgaa tggtttatct ggggcgcgtg gtttgtgcca
     9241 ttgtggttgt ggttaagtaa aagcggtttt agtgccggag aaattggctg gtcgtatgcc
     9301 tgcacagcca ttgcggcgat cctgtcgcca attctggttg gctccatcac tgaccgcttt
     9361 ttctcggcgc aaaaagtgct ggcggtattg atgttcgcag gcgcgctgct gatgtatttc
     9421 gctgcgcaac agaccacttt tgccgggttc ttcccgttac tgctggccta ctcgctaacc
     9481 tatatgccga ccattgcgct gactaacagt atcgcttttg ccaacgtgcc ggatgtggag
     9541 cgtgatttcc cgcgcattcg tgtgatgggc actatcggct ggattgcctc tggtctggca
     9601 tgtggtttct tgccgcaaat gctggggtat gccgatatct caccgactaa catcccgctg
     9661 ctgattaccg ccggaagttc tgctctgctc ggtgtgtttg cgtttttcct gcccgacacg
     9721 ccgccaaaaa gcaccggcaa aatggacatt aaagtcatgc tcggcctgga tgcgctgatc
     9781 ctgctgcgcg ataaaaactt cctcgtcttt ttcttctgtt cattcctgtt tgcgatgcca
     9841 ctggcgttct attacatctt tgccaacggt tatctgaccg aagttggcat gaaaaacgcc
     9901 accggctgga tgacgctcgg ccagttctct gaaatcttct ttatgctggc attgccgttt
     9961 ttcactaaac gctttggtat caaaaaggta ttattgcttg gtctggtcac cgctgcgatc
    10021 cgctatggct tctttattta cggtagtgcg gatgaatatt tcacctacgc gttactgttc
    10081 ctcggtattt tgcttcacgg cgtaagttac gatttttact acgttaccgc ttacatctat
    10141 gtcgataaaa aagcccccgt gcatatgcgt accgccgcgc aggggctgat cacgctctgc
    10201 tgccagggct tcggcagttt gctcggctat cgtcttggcg gtgtgatgat ggaaaagatg
    10261 ttcgcttatc cggaaccggt aaacgggctg actttcaact ggtccgggat gtggactttc
    10321 ggcgcggtga tgattgccat tatcgccgtg ctgttcatga tttttttccg cgaatccgac
    10381 aacgaaatta cggctatcaa ggtcgatgat cgcgatattg cgttgacaca aggggaagtt
    10441 aaatgaaaac agaacgtatt ctcggtgctc tttatgggca ggcgttaggg gatgcgatgg
    10501 ggatgccctc cgagctttgg ccacgcagcc gcgttaaagc acactttggc tggattgacc
    10561 gttttctgcc tggaccaaag gagaataacg cggcctgtta ttttaaccgc gccgaattca
    10621 ccgacgatac ctcgatggcg ctgtgtctgg cggatgcgtt actggaacgt gaaggcaaga
    10681 tcgatccgga tctgattggg cgtaatattc tcgactgggc gctgcgtttc gacgccttta
    10741 acaaaaacgt actaggtccg acctcgaaga ttgcgcttaa cgccattcgc gacggtaaac
    10801 ccgttgctga actggaaaac aacggcgtga ccaacggcgc agcgatgcgc gtctcgccat
    10861 taggttgttt gcttccggcg cgtgatgttg attcctttat tgatgatgtg gcgctggcgt
    10921 cgagcccgac ccataaatcc gatctggcag ttgcaggtgc ggtagtcatc gcatgggcga
    10981 tttctcgtgc cattgacgga gaaagctggt cagcgattgt tgattccctg ccttcaattg
    11041 cgcgacatgc acagcaaaaa cgcatcacta ccttcagcgc ctcactggca gcacgtctgg
    11101 agattgcgct gaaaattgtg cgcaatgccg acggcaccga atccgccagc gaacagcttt
    11161 accaggtcgt tggcgcaggt accagcacta ttgagtccgt tccgtgcgcc attgcgctgg
    11221 ttgaactggc acaaaccgac ccgaatcgct gcgccgtcct gtgcgctaac cttggcggcg
    11281 acacagacac catcggcgct atggcgacgg cgatttgcgg cgcgttgcat ggcgttaacg
    11341 ctatcgatcc tgcattaaag gcggaactgg atgcggtaaa tcagcttgat ttcaaccgct
    11401 atgccacagc gctggcgaaa tatcgtcaac aacgggaggc ggtatgagcg gcgctcgatt
    11461 acacacgctg ctgcctgaat taacctcgcg tcagccggtg atggtcgtcg gcgcggcggt
    11521 cattgatgtg atcgccgacg cttatgccct cccctggcgt gggtgcgata tcgaactgaa
    11581 gcagcagagc gttaacgttg gcggctgcgc actgaatatt gccgtggcgt taaagcgcct
    11641 cggcatcgaa gcgggtaatg ccttgccgct cggtcagggc gtgtgggcgg agattattcg
    11701 caaccggatg gcgaaagagg ggttaatcag cctgatcgat aacgccgaag gcgataacgg
    11761 ctggtgtctg gcgctggttg agccggatgg cgaacgcact tttatgtcat tcagcggtgt
    11821 ggaaaatcag tggaatcgcc agtggctggc gcgattaacc gttgcgcctg gcagcctgct
    11881 ctatttttcc ggttatcaac tggcctcgcc ctgcggcgaa ttgttagtgg aatggctgga
    11941 aaagctgcaa gacgtgacgc cgtttatcga ttttggccca cgtatcggcg atatcccgga
    12001 cgcattactg gcgcggatca tggcctgtcg acctttagta tcactcaatc gtcaagaggc
    12061 tgagattgcc gccgaacgtt ttgctttatc cgcagagata acaacacttg gcaagcaatg
    12121 gcaggagaaa tttgccgcgc cgttgatcgt tcgtctcgat aaagagggtg catggtattt
    12181 cagcaacgac gcttctggct gcattccggc atttccaacg caagttgtag acaccattgg
    12241 ggcgggcgac agtcatgcag gtggcgtact tgccgggctg gcctctggtc tgccactggc
    12301 ggatgccgta ttactgggca atgcagtggc gtcgtgggtt gtcgggcatc ggggcggtga
    12361 ttgtgcgcca acgcgcgagg aactactcct cgcacacaaa aacgtataga tcgctgcgac
    12421 agtggctaat gctgtactca ataggccgtt gctgttggtc aagcgccact tgcttgatca
    12481 ccagcaccgg tattttgcta tctaactgaa tatgtgactg aaactcggca tccggcatcc
    12541 gggcgctaac gcgggaacgc gtacgttgtg ggtaaatgtg ctggctgcgg aaatagtcat
    12601 aaagtgaaat cccgatggca tcaacatcat gaattaaatg cgccggaacc cacgattcct
    12661 caatcgatac cgcttcttca tcgacatagc gaatacgctt aagcaaaaag acatcacttc
    12721 ctgcttcgac cgccagctgc tgcgcgactt cttcgggaca tttcacgaca cgcttgttaa
    12781 cccatagcgt atcgggcttt ttcccgcgca ataccacctg ctgagaaaaa cctcgcgctt
    12841 ctttcaacga gtattcgaag atgttgttga tctgcgtgcc gtaaccgcgc gaacgcgtca
    12901 ccacaccctc ttcttccagc gcctgcatcg ccttgcgcac cgtaatgcgc gaaacgccgg
    12961 ttaactgact taaatcacgc tcaccgggca aaatattgcc atgctccagc accccgctgc
    13021 gcacggcatt ttttaccgtt tcggcaaact taatataaag cggcgtgtta tccgccgcta
    13081 aaatacgctc gttcagttgg gcaatcagct gggtatgcgc ttgttccatt tgtctttttc
    13141 ctgccgggga gtctggtggg agtatatcac tgcttcttat ttaaggcgtt tctttaatcc
    13201 catcaacgaa tgcctgcaac tcctccaccg tgccatgaaa tacattaaaa tccaccggac
    13261 cattgatgcc atctacctgt ccacggtcgg aatgctgcca gaagcgccag gccatgccgt
    13321 cattgtccgg acgacgttga taatagtgag ccacccacca cggatattca ttgaaatagc
    13381 ccgccagatt ggtgtgataa aaaacggctc ctgagtaaat aatcggcttt tttcccgtac
    13441 ttttttcgac catttttagc cactgactta ctcgtttgcg taattctttt gccgataatt
    13501 ttccccgttc ttctacgtcc agtaccgcag ggaaatcgcc ttgtgagaaa tccaccgttt
    13561 gcagaaataa tcgtgcctga accggcgctg ctaccgacgg ggagaaataa tgatacgccc
    13621 cgcgcagcag gccattttcg cggcttagtt gccagttacg cgaaaaatac gggtccacca
    13681 gcttttcgcc ttccgtcgcc ttaataaaag caaactgtaa gcggataccg ttgtcgcgca
    13741 tttttgccac ccgctgccag tcgatccgct cctgccagcg agaaacatct attccgtgaa
    13801 tggtgtaact ggcaggaatg cgaatagcga aagatttaac cgggcgataa ccgtagaaat
    13861 ggatgtagtc tgtaatctga actgctgtcg aatagaaaaa attcaccgtc tgacgcggat
    13921 aaattgcaac gatggaaatt agcccaagag cgcataatag agaggtgagt ttttttcgac
    13981 tggtaattct tagctgcatt ggtttcatcc ctgaatgtca gtgccaaagg ctgacaataa
    14041 ccaaagcagc tatagtacgg tgctcaccac caggcgtgga agtggtgaac cggaccaata
    14101 ccgtgaccaa cttccagcgt gtcggcctgg gctaatgctg atgaaagcca gctttttgcc
    14161 tcctgtacgg tgtcagccca gtttgtatgg cgcgggcgta gtgcagccaa cgccgcagag
    14221 agcgtacaac cagtgccgtg ggtgtttttg gtcataatgc gcggtgcggt aaaccgttgt
    14281 tcaccctcgc gggtaaacag ccagtccggg ctttgctcat catccagatg accacctttc
    14341 attagcactg cgccacagcc catcgccaac agcgatcgcc cttgttccag catttcctgt
    14401 tcggtgcgcg cgtgtggcgc gtcgagcaag gcggcagctt cgggcaagtt tggcgttatt
    14461 aatgaaacct gtggcaataa tcgactgcgc agcgtagcaa ccgccgaagg tgaaagcagc
    14521 gggtcgccgc tttttgccag cataacggtg tcgagtacca cgttttggat ctgataacgt
    14581 tgcaaccgtt ctgccaccgc ttcaacaata tcggtttccg ccaacatacc gattttagtg
    14641 gtgtcgattc gcacatcgct gaacaccgaa tcgagctggg cggcgacaaa atcaggctca
    14701 atgcgataca ccgactgtac gccacgggta ttttgcgcca ccagtgcagt aataactgag
    14761 caaccataag cgccaagtgc cgagaaggtt ttaagatcgg cctgaatccc cgcaccacca
    14821 ctgggatcag taccggcaat cgtcagagcg ttaattcgtt tcatgcctgc acctcctgcg
    14881 tcaattgcca gagcgcatca aggaaatgtg gaacaaaact gcctggcccc tcgcttctgg
    14941 cgactgcgcg ctctccggct tgtttcatcc agtgacaggc agatgcgaca ttttccagcg
    15001 tatcgcctgg taacgcacag caggcagcga caaccgccga taatgcacag ccagttccta
    15061 ccactttggt cattaacgga tcgccaccgt gaatgccaac ggcgcgatgg ccatcggtaa
    15121 cataatccat ctcgccagtg accacgacga ttgcgccagt ttcccgtgcc agtgtttgtg
    15181 cagcgggtat cgcgttagct gcggcgtcag tggtatccac tccccgtcca ccattagcaa
    15241 tgccagctaa tgccatgatt tccgaagcat taccacgtat ggcggttggc ttatgagaca
    15301 gaagttccag acaaaaacgg cggcgataat cgagtgcacc aaccgctact ggatcaagcg
    15361 tccagggtgt ttgagagctt tttgcttgct caacggcggc acacatcgac tgtgcgcgta
    15421 gctgcgtcag cgtgccaacg ttaatcagca acgcactggc aatagccgca aactgactgg
    15481 cctcttcggt ttcgataacc atcgctggcg atgcaccgag cgccagcaag gtattggcag
    15541 taaaggtttg caccacatcg ttggtcatgc agtgcacaag aggggaatgt tggtgaaaaa
    15601 ggtgtaacgc gtgcgcagat tgcgctgaac tcagcaggtc gacttgcata gtttgctcct
    15661 gccataacgt gaagaagcaa tgacctggtg gtccgtgact tccctacgct ggcattatcc
    15721 agatcaggtg atacgggtat ttctcagcct tcacgcagaa gggcaccccg agtcgtttgg
    15781 ttgcgatgat aaatcgcaga ggaggatggt aatgtccagc gcacgcgttg taaacgagga
    15841 aaaacggcag ttttacaatc gcgttaataa attatttgat atatgaatcc agcaccttca
    15901 gaacgacatc cagatcttct tcacgtttta gctcatcccc ctggtgaacg atgtgttccg
    15961 tcagatgacc tttaatcact tcccgcatca gaccgtttac cgcgccacgg atagcagcaa
    16021 tctgttgtaa aactgcagcg cattcgtgcg gttcgtcgag cattttcttg agcgccacga
    16081 cctggccctg aatcttactg gcacgcgctt tcagtttctg tttatcacgg attgtatgag
    16141 acatggcaac acctggttaa caagaatatg aaaaatcata gcactattaa tctactgggg
    16201 ggtagtatca ggtactgggg gggagtagaa tcagattgcc gaattaatac taagaattat
    16261 tatcatgacc gaatttacaa ctcttcttca gcaaggaaac gcctggttct tcatccccag
    16321 cgccatctta cttggtgcgc ttcatggcct ggaaccaggg cactcaaaaa cgatgatggc
    16381 ggcgtttatc atcgccatca aaggcaccat taaacaggcg gtgatgctcg gactggcagc
    16441 aactatttcg cataccgcag tggtctggtt gattgccttt ggcgggatgg tggtcagcaa
    16501 gcgctttact gctcaatcag cagaaccgtg gctccagttg atttccgcag tgatcattat
    16561 tagcaccgcg ttctggatgt tctggcgtac ctggcacggc gagcgcaact ggctggagaa
    16621 tatgcacgag catgatcatg agcatcatca tcacgatcac gaagatcacc acgaccatgg
    16681 acatcatcac catcacgaac atggcgagta tcaggatgct catgcacgag cccatgctaa
    16741 tgacattaaa cgacgctttg atggtagaga ggtcaccaac tggcaaattt tgttatttgg
    16801 cttaaccggt ggccttatcc cctgcccggc agcaattacc gtgctgttga tatgcattca
    16861 gttgaaagcc ctgacactgg gcgcaacact ggtcgtcagt ttcagcattg gcctggcgtt
    16921 aacgcttgtc accgtaggcg ttggcgcagc aatcagcgtt cagcaggtcg caaaacgctg
    16981 gagcggattt aacactctcg ctaaacgcgc cccctatttt tccagtctgt tgattggctt
    17041 agtcggtgtg tatatgggcg tacatggctt catgggcata atgcgataac tcaatccttt
    17101 aggcttgcat gttatcttca gcccaggatg attcttaaat cagctatttc cgctgactct
    17161 ctgctcgcta aggatgcttt tagggcatcc tttcatttac actttttacg aaatcatggg
    17221 atcactaaca aaatatcgct tgtcagttat attgtatggc aggaaagata tgcgactgat
    17281 attacagatc cccaaagtgg agagtttatg accattaaaa ataagatgtt gctgggtgcg
    17341 cttttgctgg ttaccagtgc cgcctgggcc gcaccagcca ccgcgggttc gaccaatacc
    17401 tcgggaattt ctaagtatga gttaagcagt ttcattgctg actttaagca tttcaaacca
    17461 ggcgataccg tcccagagat gtaccgcacc gatgagtaca acattaagca gtggcagtta
    17521 cgtaacctgc ccgcgcctga tgccgggacg cactggacct atatgggtgg cgcgtacgtg
    17581 ttgatcagcg acaccgacgg taaaatcatt aaagcctacg acggtgagat tttttatcat
    17641 cgctaaaaaa agccccctca tcatgagggg gaaatgcaga caccttgtta ttttttatta
    17701 ttagccactt gctcgtcttg cttgttatta gtcgtatttc acgttgatta atgcggttgc
    17761 ctccagtgcg ccagatttaa ctttgtttgt atcgtagacg tagtaactgg cagttatcgg
    17821 aattgaaacc gttgtatctg taagttttga ctttgccgca atttctttta aatttgtgta
    17881 tcgtttactg ctgttagtga tatttaaaag tgtgactgac gaaccattac tttcaagaat
    17941 aaatcctacg ccattatctt ccgcaccttt cagtattgtg ttgctgttgg ttaacaagtc
    18001 agaacttgat aaatacacct gcattttccg tgatgtttga ttttgttgac ctaataaatt
    18061 tttacaacgc agaataatat ccccttgttt actatttatt gttgctttgt tacctgtggc
    18121 tggtaattgt gaaacgggaa tactgtctaa agtaattaat aaatcgtccg gtttgcaggt
    18181 tgtggttatc gggttgtatg acaatgtcag attaaaggaa tagacacctg cattttgtgc
    18241 caatgcattt ctgtagctat ttacacatgc atcccatgac ttgccaccta ataaacacag
    18301 tagaaattgt actgcggcac tcccgcgtgt cgatgatgaa gcactggata atgtcgcaac
    18361 tccattaatc atcactgaac cgccgccccc cgcagaaaca ttcacttttg cattactggc
    18421 cgcaggtgtc accgttaccg aataaccact ggtgaaactg gtctgattat ttgataacgt
    18481 gatgttgttt ttattggtcc attcaaaatg taaatttaat ttaatggtat cgttataaag
    18541 tccaacaata atatccgacg ggctcatagg ggtgatagta tttgtactca cgcatgacga
    18601 atcattaaaa ttatgctcat atgtcgccat ttgtgtttga gtaagtgcaa aatccccctg
    18661 aacaaccaaa ttgtgctgaa cagataactc actataacat gccgcatagc tcacaggcat
    18721 aaccatcatc attactataa ataacattat acgaatattc ataaccacct gactattact
    18781 ggcaaatata gtttctactt tcatcaatct ctttaccgaa ggtgattgtg catgaaagtc
    18841 cttgttgctt atcaattgct acattaaccg atggcggtac ttcattggtg cgaataaata
    18901 actggcttcc ctgaccgaca acgccaatat tatgaccatg gatatcattg acttcataac
    18961 caaacattaa tggttgccca tccgctctta acgcttttat aaaccagggc ttgcgctgat
    19021 cggtatcaaa attaaccagt acaaccgcgc cgcgataaga ggcggcaatt ttccggttgc
    19081 cacgtaattc tgcttcgcta tcgctttgcg acacatccag catcaggtga ttttcccgat
    19141 aaggtgtcat tccgtcgtat accaccactc cattacggtt tgttgtgcga tatttttgcc
    19201 cattgacata agcatcttta attcctggcg cattcatcac agcaaacgtt tcggaaagac
    19261 ggttcgccag attaacgccc cccgaccagg cgacaatgcc ccctgaaaca ctggctccag
    19321 cctgtcgata agtactcgac tgactataac tgccattcac tgtcgcaacc ggcgcgttcc
    19381 aggtcaaatt cgccccagct gtcgtttcat ttccctgatt ctgataactc aggttgaccc
    19441 catagttaaa ctgatcccgg cttccaacgg ttcctgataa tcccgtatta tttgaggcaa
    19501 acccctgatc atcaaacgtc gttgagttag acatatatat ttgccgacga ggcgtcgtaa
    19561 cgtcatcacc ccaatcaaag ggaatcgata taaaaatatt aaaacgtttc tcttcatgat
    19621 gattctcgtc atacgcctgg cttgccgcga ggatatagct tatccgccgc cagttattgg
    19681 aataactcaa ctgataatcc ttactgctgc cgctacgccc ccagtaatct cgccataacg
    19741 tacttaagga cacagacccc cagccttctg gcaatgactg gctcatattg gcagagaagc
    19801 tatttttacg gccaaaatcg ttctggtaat aatcggcaat gtcatagaca tcgttttcat
    19861 cacggcgata attatcttta ttgtttgccc aaacgtgatc attaaatgtc cggtaatcac
    19921 gcgatgaata acgccaggcc gccagaccaa aacgcgtcga cgtttggctc acaaatttgt
    19981 tgtaggcaat ttgataactt tgcccgtcaa acacatcgcc gttgtcttgt ttactatgcg
    20041 acttcgtggc atcgacggaa atggcaccaa tgcgtgtatt ccagccagtc cccaaagtaa
    20101 acgcgtaata attattcgcg accatcgagc caccatacag cgtcaataaa ttattaaaac
    20161 catactgata acccgcctgg acaaaatcac tttgtttgct cgccccttca atatggctac
    20221 gacccgccgc aaaatcatat ttcgatacgc cgggttgcag catatttggc actgctgcat
    20281 aaggcaccag ataggtggtt accgagccgt ccgcctcttt cacgctgaca tcaagatctg
    20341 ctccaccacc ggccaactgc aaatctgtaa tcgcgaacgg gccaggagga acctctttct
    20401 gataaaccac aaaaccgttc tgttcaatag ttaccagcgc attactctga gcaatcccct
    20461 gcacccgtgg cgtaaaattt tgtttcgagt taggcaacat ctgcatatca cgaaacaacc
    20521 gcacaccgct gaagcgaaca gaatcaaaaa tatcgcttga tgtgtacata tcacccacgc
    20581 gaagcgtgcc gagaagttgg gcaaatccac gttccagata cagggtattg cttttccaca
    20641 cccctggatt gttatttgtt ttactgaaac tggcatcaga atgcagttgc caccccagta
    20701 aatttaaccc gctgttaaaa cgtacatatg tactcttact attacccgac gctttatagt
    20761 cactgtaata ctgactcaca taataagagg tataaaacgc attaataccc cgctcccagt
    20821 tttccggtgg aacatagcca ctttccagtt cttccaccca ggcctgcggg acactgaaat
    20881 cgagacgaaa aaccccgata tcccaggtat agctcccacc ctgaacaagt tgctcaaatg
    20941 ttaaacattg cttaccgctg gcgaagttat cgctattaat gcctaaccgc ttgataactt
    21001 ctattgataa acatgtttct tgcgggttgt ctttaacaat aatctcatat ttcccgcgcc
    21061 attgcttatt gacataaata tcgatgtcat actgccccgg taagggttga ttatcatcaa
    21121 gacgaatatt tgcaacctgc tggtctttca ttccacctat cataaaatgg gtatcaaagg
    21181 tttcttcagc tgcataagct tcaatgccga gcaataacgc tacgattgct gatgcaagtg
    21241 gggtcattct caacataggc agctcctgca attaaatttt gtcactaata tagttgccgt
    21301 ggtcatcgat aatggtcaga taccagttat ttgcattatt acttttgaca ttaacactct
    21361 gactttctaa gggggcaatc ataatagttt cataattgac tttaacatta ttagctttga
    21421 catccgaaat cgtcacccaa ttagctgagt cattttttat caccaatcca ttgccactgc
    21481 gatttaccag taacttttta aaggtcgctt tatttaccgg agcaataccc gctggccggt
    21541 aaaacaactt aattctgttt tgcatcgcaa acttcagtgc atttttgcct tcttgctctg
    21601 gactatttgg tggaatatcc agaacattca gataaaaaat gctttcttta ttagtgggca
    21661 gtttattcgg cataattttg atttttactt gctgcccgga atttgcccct atttttgcca
    21721 ctggtggcgt taacatgaaa ggaacctgaa ttttttctgg tggtaacgac gtatcgccat
    21781 catcaatcca cgcctgcagc agcgaagaac ggtttccctg gttcatcaac tgcaccataa
    21841 cttctttatt ttctgccggg taaataatgc gcgtcccgta gataacaatt ccggcatgtg
    21901 caggaaggac catagaaaaa atgagtaaag ataataatcc tttcatctta atgcctctta
    21961 aattaaaaag ccgccatggg gatagcggcc atgtttatta cctggagaaa cttacaattt
    22021 tatttattaa tcctgcgcca cacgcataac cagagaagat aacacatcac ctgattgcac
    22081 tgagttatct gtcagtttca ggtattttgc tttaaacgga atagcatatt gaccttccgc
    22141 tgttttataa ctctcaatgt taaaaggtgc agaaacatcc aggacatcgc tgttatcacc
    22201 taaatagaga tagtaaccca cggtatcgtt gctactggct atctccttat cgttaccggt
    22261 tattttatcg ttggtaatca acgagagttt tacaccagca acttctttcg ggcagctaat
    22321 ttttaacatg ctgctggtta caccgactgc atcatcatag gtagaatatt tagctttacc
    22381 ttcaaagaca gtggttggta cagtcggcat attaatactt actgaatcac cacccgcttc
    22441 gaatttacac ggagattctg caatattccc cttaatagtt aatgttccgg tatcaacgtc
    22501 tgcagcaaat actccagcgc tcataaaaaa agaagaaaag acagcggcag caataattga
    22561 acgtttcata atatgttcct taataaaatt tatttttggt tgttaagagt ctgtatctct
    22621 tggaaacaaa tttctcacat caaaaaagtt gccgcaa
//
LOCUS       DAZFKM010000077        22433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFKM010000077 DAZFKM010000000
VERSION     DAZFKM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..910
                     /locus_tag="ACHKAY_003063"
     CDS             182..910
                     /locus_tag="ACHKAY_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO5701708.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            910..5760
                     /gene="ehaG"
                     /locus_tag="ACHKAY_003064"
     CDS             910..5760
                     /gene="ehaG"
                     /locus_tag="ACHKAY_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO5701709.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6129..7784
                     /gene="lldP"
                     /locus_tag="ACHKAY_003065"
     CDS             6129..7784
                     /gene="lldP"
                     /locus_tag="ACHKAY_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO5701710.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7784..8560
                     /gene="lldR"
                     /locus_tag="ACHKAY_003066"
     CDS             7784..8560
                     /gene="lldR"
                     /locus_tag="ACHKAY_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO5701711.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8557..9747
                     /gene="lldD"
                     /locus_tag="ACHKAY_003067"
     CDS             8557..9747
                     /gene="lldD"
                     /locus_tag="ACHKAY_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO5701712.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9933..10406
                     /gene="trmL"
                     /locus_tag="ACHKAY_003068"
     CDS             9933..10406
                     /gene="trmL"
                     /locus_tag="ACHKAY_003068"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO5701713.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10459..11280)
                     /gene="cysE"
                     /locus_tag="ACHKAY_003069"
     CDS             complement(10459..11280)
                     /gene="cysE"
                     /locus_tag="ACHKAY_003069"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO5701714.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11360..12379)
                     /gene="gpsA"
                     /locus_tag="ACHKAY_003070"
     CDS             complement(11360..12379)
                     /gene="gpsA"
                     /locus_tag="ACHKAY_003070"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO5701715.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12379..12846)
                     /gene="secB"
                     /locus_tag="ACHKAY_003071"
     CDS             complement(12379..12846)
                     /gene="secB"
                     /locus_tag="ACHKAY_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO5701716.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12909..13160)
                     /gene="grxC"
                     /locus_tag="ACHKAY_003072"
     CDS             complement(12909..13160)
                     /gene="grxC"
                     /locus_tag="ACHKAY_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO5701717.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13302..13733)
                     /gene="yibN"
                     /locus_tag="ACHKAY_003073"
     CDS             complement(13302..13733)
                     /gene="yibN"
                     /locus_tag="ACHKAY_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO5701718.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13978..15522
                     /gene="gpmM"
                     /locus_tag="ACHKAY_003074"
                     /gene_synonym="pgmI"
     CDS             13978..15522
                     /gene="gpmM"
                     /locus_tag="ACHKAY_003074"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO5701719.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15556..16815
                     /gene="envC"
                     /locus_tag="ACHKAY_003075"
     CDS             15556..16815
                     /gene="envC"
                     /locus_tag="ACHKAY_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO5701720.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16819..17778
                     /gene="yibQ"
                     /locus_tag="ACHKAY_003076"
     CDS             16819..17778
                     /gene="yibQ"
                     /locus_tag="ACHKAY_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO5701721.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17765..18799)
                     /gene="waaH"
                     /locus_tag="ACHKAY_003077"
     CDS             complement(17765..18799)
                     /gene="waaH"
                     /locus_tag="ACHKAY_003077"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO5701722.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19050..20075)
                     /gene="tdh"
                     /locus_tag="ACHKAY_003078"
     CDS             complement(19050..20075)
                     /gene="tdh"
                     /locus_tag="ACHKAY_003078"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO5701723.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20085..21281)
                     /gene="kbl"
                     /locus_tag="ACHKAY_003079"
     CDS             complement(20085..21281)
                     /gene="kbl"
                     /locus_tag="ACHKAY_003079"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO5701724.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21556..22413)
                     /gene="yibB"
                     /locus_tag="ACHKAY_003080"
     CDS             complement(21556..22413)
                     /gene="yibB"
                     /locus_tag="ACHKAY_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO5701725.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
BASE COUNT         5732 a         5785 c         5647 g         5269 t
ORIGIN      
        1 tatattcata caaacacaga catcttttga aacgaatgat acatttttat gatgaatgct
       61 taaaacaata tttaatcgac acacatcact gtcattaatg taaagcaaca tagatcacca
      121 ataatacaat ggacaactct accggcattg atcgctggtc ggaggaaata aggattttta
      181 catgaacttg aagaagattt ttttcagtgc tgtcactgtt tcagttctct gcgcattaac
      241 gggatgtgac tacattgaag aaggcaagcc agaaagtagt ttattgaaac aacaagaaga
      301 acataacaat aaaattgatc tacttgaaaa acaacaagca cagctaaaga gtcaactgga
      361 aactattcaa aaacaacaaa ctggcataat aagcagtaca aagactttaa ctcacgtaat
      421 taaatccgtt aaagatcaac aaaacacttt tatttttacc gagtttaacc cagcaaaaac
      481 gaagtatttc atcctgaaca atggttcggt ggctttagcg ggtcgagtgt tatccattga
      541 cgccacagag aatggcagtg ttattcatat ttcactggtc aacttattaa gtacacctat
      601 ctcaaatatt ggttttaatg cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt
      661 tgcccggtgg caacaattac ttttcaacac atcaatgaaa tccacattga aattattacc
      721 aggtcaatgg caagacatta atttgaccct gaagggtgta tcgcccaata acctgggata
      781 tctgaagtta gccatcaaca tggaaaatat tcagttcgac aatcttcctt ctgctgaaaa
      841 tcggcagaaa agaagcaaaa aaataaatgc agttagtacg cttataacaa ggaatagcga
      901 gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg gaattatact
      961 gttaccagcg aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa gctgttaatt
     1021 tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa tgccgggaat
     1081 gacaacggtc agggtgttga ttacggtagt ggatcagctg gcgacggctg ggttgctata
     1141 ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac tgctgtgggt
     1201 tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac ccatgcgatt
     1261 ggtggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag aagtatagca
     1321 ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg ttattcaaaa
     1381 gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg agcaaacgcc
     1441 attgccctgg gaaatgccac taaagctact gagattatga gtattgctct tggcgacacc
     1501 gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa gtagcgtcgc atctgaagaa
     1561 aacgcaattg ccctggggcg tagcagtgta gctagcggta ctgacagcct cgcatttggc
     1621 agacaatcac ttgccagcgc agcgaacgct attgcgatag gtgctgagac cgaagccgct
     1681 gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag ggactaatag catggcaatg
     1741 gggttcggaa gccttgccga taaagtcaat actatcgcat taggaaatgg cagccaggct
     1801 ctggcagata atgcaatcgc cataggccag ggcaacaaag ctgatggcgt ggatgccatc
     1861 gctctgggta atggtagcca gtcgagaggc ttaaacacca ttgccttagg cacagccagt
     1921 aatgcaactg gtgataagag tcttgcgctt ggtagtaata gcagtgccaa cggtattaac
     1981 tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca ataccgtctc tgtcggcaat
     2041 agttcattaa aacgcaagat cgttaatgtg aaaaatggcg cgatcaagtc tgacagttac
     2101 gatgccatta atggttcaca gctttatgcc attagcgact cggtagcaaa aaggcttgga
     2161 ggaggggctg cagtagatgt tgatgacggt actgttacag caccaaccta caatttaaaa
     2221 aatggtagca aaaataacgt aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa
     2281 tgggaccaaa ccaaaggcaa atacagcgct gctcatggta ctagtagccc aactgccagc
     2341 gtaatcaccg atgttgcgga tggcacgatt tcagcctcca gtaaggatgc ggttaacggt
     2401 tcccaactga aagctaccaa tgacgatgtc gaagccaaca ccgccaatat cgctactaat
     2461 accagcaaca ttgccacgaa tacggcaaat attgccacca ataccaccaa tatcaccaac
     2521 ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga aactaaaaag
     2581 gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt taaagatgcc
     2641 gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac caccaacgat
     2701 gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc cactaacacc
     2761 accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg
     2821 gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag caaaatcacc
     2881 aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg ctctcagctg
     2941 aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa caccaccaac
     3001 atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
     3061 gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat caccaatatc
     3121 ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca gctttatgac
     3181 ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg
     3241 tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg cgatgcactg
     3301 gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg ggatgccacc
     3361 gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat cactgatgtc
     3421 gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca actccacggc
     3481 gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc
     3541 atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg tgatgccctg
     3601 aatgctatcg ataccactct tgacgacgct ctgctctggg atgcggacgc cggtgaaaat
     3661 ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac taacgtcgct
     3721 aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact ctataccacc
     3781 aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga cggcaccatc
     3841 accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga cgccctggat
     3901 gcgcttgatg ataacgcctt gctgtgggat gagactgcca atggcggtgc tggagcctac
     3961 aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg cagtattagt
     4021 gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat gatgattgag
     4081 cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta tatccaagaa
     4141 aacggtgcgg gtattaacta tgtgcgtact aacgacgacg gcttagcgtt caacgacgcc
     4201 agcgcacagg gtgttggcgc tacagctata ggttataact ctgtcgccaa aggcgatagc
     4261 agcgtagcta ttggtcaggg cagctacagc gacgttgata cgggtatcgc cctgggtagc
     4321 agctctgttt ccagccgagt gattgccaaa ggctcccgtg acaccagcat aacggaaaat
     4381 ggcgttgtta ttggttacga caccacggat ggcgaactgc tcggtgcatt gtctatcggt
     4441 gatgacggta aatatcgtca aatcatcaac gtagccgatg gttccgaagc ccatgacgcc
     4501 gttacggttc gtcaattgca gaatgcgatt ggtgcggtcg caaccacgcc gactaaatac
     4561 ttccacgcta attcaacgga agaagattca ctggcagtgg gaactgactc gctggcaatg
     4621 ggtgcgaaaa ccatcgtgaa tggcgataaa ggtattggta tcggttatgg tgcctacgtg
     4681 gacgcgaatg cacttaacgg cattgccatt ggtagcaatg cgcaagtcat tcatgtcaac
     4741 agtattgcga taggtaatgg ttctacgacc actcgtggcg ctcaaaccaa ttataccgcc
     4801 tacaacatgg acgcaccgca gaactctgtc ggtgaattct cagtcggtag tgcggatggt
     4861 caacgtcaga tcactaacgt cgcagcaggt tcggctgata ccgatgcggt caacgtgggt
     4921 cagttgaaag taacggatgc gcaggtttcc cagaataccc agagcattac taacctgaac
     4981 aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgacatt
     5041 gtcacctccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga tgccaacgcc
     5101 cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga caacagcgtc
     5161 gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg ttcttctact
     5221 aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg ccaccgatgc ggttaacgtg
     5281 tcgcagctga aatcttctga agcaggcggc gtgcgctacg acaccaaagc tgatggttct
     5341 atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg tatcagcaac
     5401 gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg
     5461 caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa actgtctaaa
     5521 actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg tctgccgcag
     5581 gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa cggtgaatcg
     5641 gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta caaattacaa
     5701 ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat tcagtggtaa
     5761 tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc gttacgcgca
     5821 tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga tgaggattgc
     5881 ccttttcttt accagacatc tcccccccac aagaattggc cctaccaatt cttcgcttat
     5941 ctgacctctg gttcacaatt ccccaattaa aactcacatc aatattgcca atacataaca
     6001 tttagttaac cattcattgt cattatccct acacaacaca attggcagtg ccacttttac
     6061 acaacgtgtg acaaggagat gagcaacaga ctcattacac gatgtgcgtg gactccagga
     6121 gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc tggctttcca
     6181 gtctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag ctcaaactga
     6241 aaggatacgt cgccgcctca tggacggtag caatcgccct tgccgtggct ttgctgttct
     6301 ataaaatgcc ggtcgctaac gcgctggcct cggtggttta tggcttcttc tatggtttgt
     6361 ggcccatcgc gtggatcatt attgcagcag tgttcgtcta taagatctcg gtgaaaaccg
     6421 ggcagtttga catcattcgc tcgtctattc tttcgataac ccctgaccag cgtctgcaaa
     6481 tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg agccgcaggc tttggcgcac
     6541 cggtagcaat taccgccgca ttgctggtcg gcctgggttt taaaccgctg tacgccgccg
     6601 gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggc attccaatcc
     6661 tggttgccgg acaagtaaca ggcatcgaca gttttgagat tggccagatg gtggggcgtc
     6721 agctaccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg gacggctggc
     6781 gcggtatcaa agagacgtgg cctgcggtcg tggttgcggg cggctcgttt gccatcgctc
     6841 agtaccttag ctccaacttc attgggccgg agctgccgga cattatctct tcgctggtat
     6901 cactgctctg cctgacgctg ttcctcaaac gctggcaacc agttcgcgta ttccgcttcg
     6961 gtgacttggg ggcgtcacag gttgatatga cgctggccca caccggttac accgcgggtc
     7021 aggtgttacg tgcctggaca ccgttcctgt tcctgaccgc taccgtaaca ctgtggagca
     7081 tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg gtgatcaaca
     7141 ttccggtgcc gtacctcgat aaactggttg cccgtatgcc accagtggtc agcgaggcta
     7201 cagcctatgc cgccgtgttt aagtttgact ggttctctgc caccggcacc gccattctgt
     7261 ttgctgcact gctctcgatt gtctggctga agatgaaacc gtctgacgct atcagcacct
     7321 tcggcagcac gctgaaagaa ctggctctac ccatctactc catcggcatg gtgctggcgt
     7381 tcgcctttat ctcgaactat tccggactgt catcaacact ggcactggca ctggcgcaca
     7441 ccggtcatgc attcaccttc ttctcgccgt tcctcggctg gctgggggta ttcctgaccg
     7501 ggtcggatac ctcatctaac gccctgttcg ccgcgctgca agccaccgca gcacaacaaa
     7561 ttggcgtctc tgatctgttg ctggttgccg ccaataccac cggtggcgtc accggtaaga
     7621 tgatctcccc gcaatctatc gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt
     7681 ctgatttgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga
     7741 tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg ttttacccag
     7801 acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga
     7861 agcgggcatg aagttgcccg ctgagcgcca actggcgatg caactcggcg tatcacgtaa
     7921 ttcactacgc gaggcgctag caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg
     7981 cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc
     8041 gctaaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg aagcccgcta
     8101 cgccattgaa gccagcaccg catggcatgc ggcaatgcgc gccacacctg gcgacaaaga
     8161 aaagattcag ctttgctttg aagcaacgct aagtgaagac ccggatatcg cctcacaagc
     8221 ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc tgctgcaaac
     8281 catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc agcggatgta
     8341 tctggtgcca ccggtttttt cacaactgac cgaacaacat caggctgtca ttgacgccat
     8401 ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta gttttgttca
     8461 caccaccatg aaacgattcg atgaagatca ggctcgccac gcacggatta cccgcctgcc
     8521 cggtgagcat aatgagcatt cgagggagaa aaacgcatga ttatttccgc agccagcgat
     8581 tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt tccactatat ggatggtggt
     8641 gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc
     8701 cagcgtattc tgaaaaacat gtccgactta agcctggaaa cgacgctgtt taatgagaaa
     8761 ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc
     8821 gaagttcagg cagccaaagc ggcggacgcg catggcattc cgtttactct ctcgacggtt
     8881 tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg gttccagctt
     8941 tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt
     9001 tgttcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcacgcta ccgtgatgcg
     9061 cattcaggta tgagcggccc aaatgcggca atgcgccgct acttgcaggc ggtgacgcat
     9121 ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg taatatctca
     9181 gcttatctcg gcaaaccgac cggactggaa gattacatcg gctggctggg gaataacttc
     9241 gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga tggcccgatg
     9301 gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat
     9361 ggaattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgctcgt
     9421 gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga
     9481 attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg
     9541 ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta
     9601 aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcagt
     9661 gaaattacgc aagattcgct ggtgcagggg ctgggtaaag agttgcctac ggcactggct
     9721 ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa ccctctccct
     9781 cagggagagg ggaccgttcg gcgctgtatg tactccctca ctctgaaacg acaccgcact
     9841 ctttttttct ccctcgcccc tccggggtga ggggaaaagg ccgcactgct caccaatttt
     9901 ctgctatcct gcccccgcac taagggggca gtatgctaaa catcgtactt tacgaaccag
     9961 aaattccgcc aaatactggc aatatcatcc gtctttgcgc taataccggc tttcgtctgc
    10021 atatcatcga accgatggga tttgcctggg acgataagcg cctgcgccgc gcggggctgg
    10081 actatcacga gtttaccgcc gttacgcgtc atcatgactt tcgcgcgttc ctcgaagcag
    10141 aaaatcccca gcgcctgttt gccctcacta cgaaaggtac gcctgctcac agcgccgtaa
    10201 actatcagga tggcgattat ctgatgttcg gcccggaaac acgcggcctg ccagcgagca
    10261 ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc aatggtgccg gacagccgca
    10321 gcatgaacct gtccaatgcg gtgtcggtag tggtgtatga agcctggcgg cagctgggat
    10381 atccgggagc gttgttgaga gattagttac tgtatgccgg atagatgatt acatcgcatc
    10441 cggcacgatc acagcacatc agatcccatc cccatactca aatgtatggt taataccgtt
    10501 gaaatgctgg tccatatcca ttgatggctt atcgctgtct ggtttaccga caatacgagc
    10561 cggaacacca gcggcggtag tatgcggcgg caccggttgc agcaccacgg aacctgcgcc
    10621 aatcttcgcg ccgcgaccaa cttcaatatt gccgagaatt ttcgcgcccg cgccaatcat
    10681 cacaccttca cgaattttcg ggtgacggtc accaccagat ttacccgtac cgccaagcgt
    10741 cacagattgc agaatcgata cgtcgttttc aattaccgcc gtttcaccaa cgacgatgcc
    10801 cgtggcgtgg tcgagcatga taccgcgacc aatttttgct gccgggtgaa tatcgacctg
    10861 gaacgtcaca gaaacctggt tttgcagaaa gattgccagt gcgcgacgcc cctgattcca
    10921 caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg
    10981 ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc
    11041 agaggcgatc atttccgggt cagcggcgta ggcttcttca accacttcac ggatagcaat
    11101 agcaggcata attggcgatg acagcttgtt cgccagcatg tagctcaggg cactgccaag
    11161 gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc agcattggct cacagtccgc
    11221 cagcgttctg gcttcggctt taatattgtt ccagacaatt tccagttctt cacacgacat
    11281 tgcttactcc acacgatgag ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg
    11341 taacaaaggt tccctggggt tagtggctgc tgcgctcgtc cttgcgtgca cgacctagta
    11401 aagtcaatgc tgcctcgcgc gcgttttttc cgcaatataa tacttgataa atttcctcgg
    11461 ttattggcat ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc gtattgcggt
    11521 agccttccac cacctgacca atcttctcct gcgcgctttg tacatccatg ccctgaccga
    11581 gcatcatgcc aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc accagatcgc
    11641 ccagccccgc catgcccata aaggtggcag gatcggcacc cagcgccgca ccaagacgcg
    11701 acatttcagc cagcccacgg gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac
    11761 cgtcagacat ccccgcgcca atggcaatga cgtttttcac cgcgccgcca agctgcacgc
    11821 caatgaaatc cgggttgctg taaacgcgga aacttttgcc acagtgcagc aattgttgga
    11881 gatcatcggc aaaggtctgg tcggtagagg ccaacgaaat agccgtcggt aaacctgccg
    11941 ccagttcttt cgcaaacgtt gggccagaga taaccgccag cggaatttga tcgcctaagg
    12001 cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc ttccagccct ttggtcgccc
    12061 acaccagacg cgcatcagga cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga
    12121 catggctggg tacgacgacg agaatattac ggctggctgc cagcgcagtg gcgagatcgc
    12181 tttcaagatg gagcgtatcg ggaaaaggca catcggggag aaacgcggcg ttacagcggt
    12241 cgcgttcaag cgttgcgata tgttcagggt catggcccca gaggacaacc tcgtggccat
    12301 ttcttgccag ggtaatggca agagcggtgc cgtacgagcc ggcaccgatc acagtcattg
    12361 aagcattacg ttggttcatc aggcatcctg atgttcttca gtaccttcgc cagcctgctg
    12421 ctgcaaatag ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa
    12481 tgtaccgcgg gataccatgc tggtgatgca ctcacgagca tacgggaaca gaatgttcgg
    12541 gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg atacccgcga tggagaaaat
    12601 accgccctgc tgaacttcac acaggaacgc ggtttcttcg cccagggagg ccgttacggt
    12661 aacacgcagt accacttcgt atacgtcatc tgccagttgg gaagatgccg tatccagatc
    12721 aagtttaact tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa
    12781 agagatatcc ttggtataaa tacgttggat ctggaaagtc atttcagtgt tgttttgttc
    12841 tgacatgtgt agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg
    12901 ttcacacgtt atttcagcag gggatccagt ccaccacgtg catccaatgc atacaagtca
    12961 tcacagccgc caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg
    13021 cgtttgatca tctcttcacg cttggcggca ttgccatcga tcggcaactc ctgaaaactc
    13081 acgcccttgc tgctcagcag tgcttttgca cgatggcaat acgggcaggt ttctttggta
    13141 tagatttcaa cattggccat aatttagctc ctgttttttt accctgaaat ttcatgttgc
    13201 agggcggctg caaaatggag tgtccccaga agcttataac aataagtcgc tgggggccta
    13261 attgcagcta acggcctgca tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga
    13321 ggcaagtttt cgccagccca gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa
    13381 ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc gtctaccacg
    13441 ataaccggtt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt gatttcactc
    13501 ggcaacagat tgatagaacc tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa
    13561 tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg agtaatcacc
    13621 ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag taacgcgatc
    13681 caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg catggggggt
    13741 aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc
    13801 aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc
    13861 aaatctggca tcggcttgcc gcgagcggtc gtatattttg atcttcagag gctattttat
    13921 cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg
    13981 tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta tcgtgaagaa
    14041 cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact gtgggccaat
    14101 cgtccgcata ccctgatcga cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg
    14161 ggtaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg
    14221 actcgtctgg acgttgaaat caaagatcgc gctttctttg ctaatccagt gctgactggc
    14281 gcggtagata aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct gctctccgca
    14341 ggcggcgtac acagccacga agatcacatc atggcgatgg tagaactggc agctgaacgc
    14401 ggcgcagaaa aaatctacct gcacgcattc cttgacggtc gcgacacccc gccgcgcagt
    14461 gctgaatcct cgctgaaaaa atttgaagag aaatttgctg cgctgggtaa aggccgcgta
    14521 gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga tcgcgttgaa
    14581 aaagcctatg acctgctgac tctggcgcag ggtgagttcc aggccgatac tgccgttgct
    14641 ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt
    14701 cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg atgcgctgat tttcatgaac
    14761 ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg tgaacgctga tttcgatggc
    14821 ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac
    14881 atcaaaactg cggttgctta cccacccgct tccctggtta acaccttcgg cgagtggatg
    14941 gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact
    15001 ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac
    15061 tcaccgaaag tggctaccta cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa
    15121 aaactggttg cggccatcaa gagcggcaaa tacgacacca ttatctgtaa ctatccgaac
    15181 ggcgacatgg taggtcacac cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg
    15241 gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca actgctgatc
    15301 accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca ggcacacacg
    15361 gcacacacca acctgccagt tccgctgatt tatgttggtg ataagaacgt gaaagcggtt
    15421 gaaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg tatggaaatc
    15481 ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc catgagggga
    15541 aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat caggcccatc
    15601 atctacgcca gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat
    15661 gagcgtgacc aactcaaatc tattcaggcc gatatcgccg caaaagagcg cgcggtacgc
    15721 caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga agaagcgatc
    15781 tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact caataaacag
    15841 attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc
    15901 agcctcgccg cgcaactgga tgccgcattc cgtcagggcg agcataccgg tattcagcta
    15961 attctcagcg gtgaagaaag ccagcgtgga cagcgtttac aggcttattt cggctatctc
    16021 aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg
    16081 cagcgtgctg aactggaaga gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc
    16141 gcccaacagg cgaaactgac tcaggctctg aacgagcgta aaaagacgct ggcggggctg
    16201 gaatcttcca tccagcaagg tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt
    16261 ctgcgtaaca gcattgcccg tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg
    16321 cgtgaggccc aggcggttcg cgaccgccag aaagaagcga cgcgcaaagg caccacctat
    16381 aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt
    16441 ggtcaggcat tctggccggt tcgcgggccg acgctgcatc gctatggtga acagctacag
    16501 ggcgaactac gctggaaagg aatggttatc ggtgcctctg aaggtactga agttaaagcg
    16561 attgccgacg gtcgggtgat tctggctgac tggctgcaag gttacggtct ggtggtggtg
    16621 gttgagcacg gtaaaggcga catgagtctt tacggctata atcagagcgc actggtgagc
    16681 gttggttcgc aggttcgcgc gggccagcca attgcactgg tgggcagcag tggcggtcag
    16741 ggtcggcctt cactctattt cgaaattcgc cgccagggtc aggcggtcaa tccacagccg
    16801 tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg
    16861 ttggcgctct cctcccccgt acttgctggc aaacttgcca tcgtcattga tgattttggg
    16921 tatcgcccgc acaacgaaaa ccaggtgctg gcgatgccct ccgctatctc cgtcgctgta
    16981 ttacccgatt caccgcacgc cagagagatg gcgaccaaag cgcataacag cgggcacgaa
    17041 gtgttgattc atctcccgat ggcaccactg agtaaacagc cgctggagaa aaatacgctg
    17101 cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt caataacgtg
    17161 ccctatgccg tggggatcaa caaccacatg ggtagcaaga tgacctctaa cctgtttggt
    17221 atgcagaaag tgatgcgggc actggagcgt tacaatcttt acttcctcga cagcgtaacc
    17281 atcggtaata cccaggcgat gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg
    17341 aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca
    17401 attgatctgg cgcgtcgcaa cggttcaacc attgccattg ggcatcctca cccttcaacg
    17461 gtacgcgtgc tacaacagat ggtttataac ctgccgccag acattacgct ggtgaaagcc
    17521 agcagcttgc tgaatgaacc gcaggttgat acttctacac cgccgaaaaa cgctgtgcct
    17581 gatgcaccgc gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa
    17641 cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg
    17701 atcgtttact tccagcatca gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt
    17761 aacgctagcg caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc
    17821 gccattgcca taagcggaaa gaccacagta acgcctggta accgaccttc acgctgcgca
    17881 cattggtaat caggcgctta tacataccgg aagtaaatat ctcggcaatc atccgttggc
    17941 gggtaagaat atccggctct ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta
    18001 tttgctgatg aaattccgga taaatcgtaa ttttgtcggc ataatttcga tttaatttct
    18061 ccagcaggcg ggtaatctta atatagtgac gttgataatt aaggttttta ttcccttgtc
    18121 tatgcaaccg actcactgac gtattatgca gataatattt atataatgat tgctcggtat
    18181 atcgcgcacg cagcgcgtta aacatgaatt ctgttgtcca gacaatatcc tgatgatgta
    18241 atccggcaat aaatttaatg ttatttttaa caataacatc acggcgataa acccccatcc
    18301 agacaacgtg agtccaacgg cgcgaagaaa gccccatccg cagccagtcc gggccggtta
    18361 atacgccggt tgagcgaagg cgatcgctgg ggatggattg ccaggtttct cccgtttcac
    18421 gaaaacacca gtcagcgttg cactgcgcca cgtcgaggtc atcctctaac gccatggtca
    18481 tcagcgtttc gtacatggtg ggatagacct catcgtcagc atcgacaaaa gcgacatatt
    18541 tgcccgtcgc cacctcgatc ccacgattac gcgctaccga tgcgccagca ttcgcctgat
    18601 gcaacaaacg aacgtgcgga tagttttctg cgtaatgctt tgctatttca acagaattat
    18661 ccgttgaacc atcgttaata ataatgattt ccagagcagt ccaggtttgc gtaattaaag
    18721 attccataca agtgcggaaa tcatcgcccg cattatataa cggaataata acactaagtt
    18781 tattggtgct gttcatcata aattaccgac gacctctgaa gttatcctga cgttttacag
    18841 gctttgtatt aagaaactat taagcctgtg agatattatt tttacaatta tttttacagg
    18901 tgagagagtc acttcagggt agagagataa tgagagcagc tatataagag atcagcgcaa
    18961 ttacgatatc tttattacac tgacaggatt ttacatattt aacggattat cctcaggcaa
    19021 aagggctgga ataccagccc ttgttcgtgt taatcccaac tcagaattac tttcccggac
    19081 tggcccgaac gcatagcgtc aaagcccttc tggaaatcat cgatagagaa acgatgggta
    19141 atgatcggcg agagatccag acctgactga atcagcgccg ccatcttgta ccaggtttca
    19201 aacatctcac gaccgtaaat acctttaatg aacaagcctt taaagattac tttggtccag
    19261 tcgatagaca tatcggacgg tggaataccc agcatcgcaa tacgaccgcc gtggttcatg
    19321 gtatcgagca tggtacgaaa cgctggcggc gcgccggaca tttccagacc gacatcaaag
    19381 ccttcggtca tgcccagttc agccatcaca tcattaaggt tttctttcgc gacgttaacc
    19441 gcacgggtga tacccatttt gcgcgccagc tcaaggcggt attcgttaac atcggtgatc
    19501 accacattgc gtgcaccaac gtgtttcgcc accgccgcag ccataatacc tatcggacct
    19561 gcaccagaaa ccagcacatc ttcgcccacc agatcgaacg acagcgcggt atgcacggcg
    19621 ttaccgaagg gatcaaaaat ggaagccagg tcgtcggaga tattgtctgg gattttgaag
    19681 gcgttgaacg ccgggatcac cagatattcg gcgaagcagc ccggacggtt aacgccgacg
    19741 ccgatcgtgt tgcggcacag atgggtacga ccgccacgac agttgcgaca gtgaccgcag
    19801 gtgatatggc cttcgccaga aacgcgatcg ccgattttga agcctttcac ttcctgacca
    19861 atacctacca cttcaccgac atattcatgg cctacgacca tcggcaccgg gatggttttt
    19921 tgcgaccact catcccagtt atagatgtga acgtcagtcc cgcagatggc tgttttacgg
    19981 attttaatca gcagatcgtt atgcccgagt tccggtacag gaacgtcggt catccagatg
    20041 ccctcttccg ctttcagttt ggataacgct ttcatctcac atcctcaggc gataacgccc
    20101 agttgtttac caatacgcgt gaatgcttct actgcacgcg taatttgctc aggggtatgc
    20161 gccgcagaca tctgggtacg aatacgcgcc tgacctttcg gaacgaccgg atagaagaaa
    20221 ccggtgacgt aaatgccctc tttttgcagc tcacgggcaa atttctgcgc cactactgca
    20281 tcgccaagca tgaccggaat aatggcatga tcggctcccg ccagggtaaa gcccgccgcc
    20341 gacatttgct cacggaactg gcgcgcgttc gcccacagac ggtcacgcag ttcgctgccc
    20401 gcttcgacca tctccagtac tttgatggac gcggcaacaa tggccggtgc cagcgagttg
    20461 gagaacaggt acggacgaga acgctggcgc agccactcaa ccacctcttt gcgcgccgcg
    20521 gtataaccac cagaagcccc gcccagcgct ttaccaagcg taccggtgat aatatcgacc
    20581 cggcccatca catcgcagta ttcatgggaa ccacgaccat tttcaccgac aaaaccgacc
    20641 gcgtgggagt cgtctaccat caccagggca tcgtatttat ctgccagatc gcaaacgccc
    20701 ttcagattgg caatcacgcc gtccatcgag aatacaccat cggtggcaat cagcacatga
    20761 cgcgcaccgg cttcacgcgc ttctttcaga cgagcttcaa gctcctgcat atcgttgttg
    20821 gcatagcgat agcgtttagc tttacacaga cgcacaccat caataataga cgcgtggttc
    20881 agtgcgtcgg agataatggc gtcttccgcg cccagcagcg tttcaaacag gccaccgtta
    20941 gcatcaaagc aggaagagta gagaatcgca tcttccatcc ccaggaaggc cgccagtttt
    21001 tgttcaagct ctttatggct gtcctgagtg ccgcaaataa aacgcaccga agccatgccg
    21061 aaaccgtgag aatccattcc cgcctttgcc gccgcaatca gatcaggatg attcgccagc
    21121 ccgagatagt tgttggcaca aaagttaatg acgtggcttc catcagccac agtgatatct
    21181 gcttgctgcg cagacgtaat aatgcgctct tctttaaaca acccttccgc ccgtgcggtt
    21241 tccagatcgt tggttaactg ctgataaaat tctccacgca ttgcgattct ccagacaggg
    21301 caaattccag cacatattac ccaaacttat aggtcgcgac gagatgacgc gttaacactt
    21361 ctgcaaaatt caggataaat aacgtgtaac ttgatgtctc tcaacaccta cagatagtag
    21421 aaaaactgta cagttttggc tgggcataat gatgattgat tttactgcat tgatataaaa
    21481 agaaaaaatg atgccaaagc atcatttttc ctatctcagt tggtatcaaa acgctaccca
    21541 ctaatggagg gaaacctatt ttcttgaaag gaaaattctc agcgcctgca tttttgcccc
    21601 taaagtgtta ctcctgaagc agcgaaacaa atcgaaccat tttcctctcc ccagatagtt
    21661 taggttgaag agatcggggc gtttgtaata gcacatgaca aaaatgccct ggtcatcatc
    21721 cacaatgtta ttatttagtg ttattttttg actttccaga actaatttat agaattcttt
    21781 ccatttatgc tgtgaaccaa caattgcgcc cccaataata tatacatgat tgccaatcat
    21841 gaaatcaaaa acctgttgtt gagatgtgac agtaagccct ttttttatag tgaaaagatg
    21901 catcttactt tcatcaaatg ggaaatccca aattttcaat cctcgagtta catttggctt
    21961 acggcaatag ccaaaatcta tccacgcaac aagaggcgtt ttcactaagc ccatgttgat
    22021 agctttattt acaaaatagg ccttaaggtt acataccaaa acatactctg gtgaccagta
    22081 ctctgggttt tttaattgtc gaggctcaag tctatttgta aacgactcat ctttttgaat
    22141 tttttcgata cgacttctga tatatctaaa tttctttttt atatcgataa cgataaccgt
    22201 tgttggtttt ccgttgcgaa tcgcttcaac ccttggtttc aaatcgggag aagtgaaaat
    22261 aatcatctca ttttcaagtg cggccagacg ctcaaagtaa ctaaaataaa catcaactga
    22321 acgagctagt ttttcacgga accctttatt agcagtccaa tcccctctac caatgtcaaa
    22381 atatgcagtg ataattgtgg tggatgattt cattactaat aagaccatta ata
//
LOCUS       DAZFKM010000078        22237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFKM010000078 DAZFKM010000000
VERSION     DAZFKM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..906
                     /locus_tag="ACHKAY_003081"
                     /pseudo
     CDS             1..906
                     /locus_tag="ACHKAY_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(1060..2709)
                     /gene="yjcE"
                     /locus_tag="ACHKAY_003082"
     CDS             complement(1060..2709)
                     /gene="yjcE"
                     /locus_tag="ACHKAY_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5701726.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2861..4210)
                     /gene="ghxP"
                     /locus_tag="ACHKAY_003083"
                     /gene_synonym="yjcD"
     CDS             complement(2861..4210)
                     /gene="ghxP"
                     /locus_tag="ACHKAY_003083"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO5701727.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4756..5220)
                     /gene="soxR"
                     /locus_tag="ACHKAY_003084"
     CDS             complement(4756..5220)
                     /gene="soxR"
                     /locus_tag="ACHKAY_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO5701728.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5306..5629
                     /gene="soxS"
                     /locus_tag="ACHKAY_003085"
     CDS             5306..5629
                     /gene="soxS"
                     /locus_tag="ACHKAY_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO5701729.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5632..7218)
                     /gene="pdeC"
                     /locus_tag="ACHKAY_003086"
     CDS             complement(5632..7218)
                     /gene="pdeC"
                     /locus_tag="ACHKAY_003086"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO5701730.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7648..7929
                     /gene="yjcB"
                     /locus_tag="ACHKAY_003087"
     CDS             7648..7929
                     /gene="yjcB"
                     /locus_tag="ACHKAY_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO5701731.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(8028..8564)
                     /gene="ssb1"
                     /locus_tag="ACHKAY_003088"
     CDS             complement(8028..8564)
                     /gene="ssb1"
                     /locus_tag="ACHKAY_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO5701732.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8818..11640
                     /gene="uvrA"
                     /locus_tag="ACHKAY_003089"
     CDS             8818..11640
                     /gene="uvrA"
                     /locus_tag="ACHKAY_003089"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO5701733.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11675..12031)
                     /gene="yjbR"
                     /locus_tag="ACHKAY_003090"
     CDS             complement(11675..12031)
                     /gene="yjbR"
                     /locus_tag="ACHKAY_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5701734.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12035..12451)
                     /gene="yjbQ"
                     /locus_tag="ACHKAY_003091"
     CDS             complement(12035..12451)
                     /gene="yjbQ"
                     /locus_tag="ACHKAY_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO5701735.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12562..13275)
                     /gene="aphA"
                     /locus_tag="ACHKAY_003092"
     CDS             complement(12562..13275)
                     /gene="aphA"
                     /locus_tag="ACHKAY_003092"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO5701736.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            13677..13899
                     /locus_tag="ACHKAY_003093"
                     /pseudo
     CDS             13677..13899
                     /locus_tag="ACHKAY_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14402..15595)
                     /gene="tyrB"
                     /locus_tag="ACHKAY_003094"
     CDS             complement(14402..15595)
                     /gene="tyrB"
                     /locus_tag="ACHKAY_003094"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO5701737.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(15830..16927)
                     /gene="alr"
                     /locus_tag="ACHKAY_003095"
     CDS             complement(15830..16927)
                     /gene="alr"
                     /locus_tag="ACHKAY_003095"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO5701738.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(16980..18395)
                     /gene="dnaB"
                     /locus_tag="ACHKAY_003096"
     CDS             complement(16980..18395)
                     /gene="dnaB"
                     /locus_tag="ACHKAY_003096"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5701739.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            18478..19461
                     /gene="qorA"
                     /locus_tag="ACHKAY_003097"
     CDS             18478..19461
                     /gene="qorA"
                     /locus_tag="ACHKAY_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO5701740.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(19627..19869)
                     /gene="pspG"
                     /locus_tag="ACHKAY_003098"
     CDS             complement(19627..19869)
                     /gene="pspG"
                     /locus_tag="ACHKAY_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO5701741.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(20003..21040)
                     /gene="dusA"
                     /locus_tag="ACHKAY_003099"
     CDS             complement(20003..21040)
                     /gene="dusA"
                     /locus_tag="ACHKAY_003099"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO5701742.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(21403..22110)
                     /gene="yjbM"
                     /locus_tag="ACHKAY_003100"
     CDS             complement(21403..22110)
                     /gene="yjbM"
                     /locus_tag="ACHKAY_003100"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO5701743.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(22134..>22237)
                     /gene="yjbL"
                     /locus_tag="ACHKAY_003101"
     CDS             complement(22134..>22237)
                     /gene="yjbL"
                     /locus_tag="ACHKAY_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO5701744.1"
                     /translation="AALRYRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         5875 a         5681 c         5284 g         5397 t
ORIGIN      
        1 gtcgccgtcc ataataatac tatcgaaatt ccacagacag taaatggtgg ttacgatttt
       61 tcacacctta gcctgaaagg tatcgtgatt aaagatgaag atttatccaa ttcgaatttt
      121 gcaggttgct gactacaaaa cgctattttc caggactgta atatgtataa aacgaatttt
      181 tattacgcca taatggaaaa aatacttttt gataattgta ttctcgatga ctcaaatttc
      241 gctcagataa aaatggccga cggaactcta aatgcatgct ccgctatgca tgttcaattc
      301 tacaatgcag caatgaatag agccaatatt aaaaatacct ttcttgacta ttcaaatttt
      361 tatatggcgt acatggctga ggtaaatctt tataaagtaa tagcgccata tgttaattta
      421 tttaaagccg accttagttt ctctaaactc gatttaatta actttgaaca tgctgatctg
      481 tctcgcgtca atctgaacaa agcaatcctc cagaatataa acttaattga tagcaaactc
      541 ttttgtacgt ggctaacaaa tacattcctc gaaatggtta tatgtaccgg ctctaatatg
      601 gctaatgtta attttaataa tgccaattta agcaactgcc atttcaactg ttctatttta
      661 acaaaagcct gtatgtttaa tacccgtctc tatcgggtta attttgatga ggctagcgtc
      721 cagggaatgg gcatttctat tctccgtggg gaggaaaata tccccattga tagtgatacc
      781 ctggtaacac tacagaaatt ctttgaagaa gattgtacct ctcatactgg catgtcacaa
      841 actgaggata atattaatgc agtcgctatg aagattactg cagatattat gcaacacgca
      901 gattgaggtt aagtcgaaga acagccaatg aattgacttt acaagagaga taacttattt
      961 gttttagaat catgaatgtt agcccgagcc aacctgttaa aggtgcaaaa gattgcacct
     1021 ttttcagatt acctcgggcc tggttacgtc gctttcggct tactgatttt cctcaattag
     1081 caacgcttca agcaaatcga gatcgtgcag taatttttgc agcgtttcgt tgctgatctc
     1141 ccgcgtggcg cgcaggtggt acagttcagc acgttcagaa cgcaatgccg ccagacggaa
     1201 gcgacgctca aggttctctt cctgcacgga actttcaacg tcgttacgtc catcggcgcg
     1261 acgacgcagg ttaccaatga cgcgagaact gacctccgta agcagctggt tatcgatatt
     1321 ctcttcggta tccgccgcca gacgctcctc cattttctgg atcgccacaa tcgccacttc
     1381 tgccgttgcc gctcgcgcaa tacgttcttc tttcaattgt tgcgaatggt ccgcgacttc
     1441 aatgtgttgt agcagaatag gcaacatcac cacgccgaca aacagcgaga agagaatgac
     1501 gccagccgcc aggaacacca gctcatagcg cgccgggaag acgttaccat ccggcaagag
     1561 cagcgggata gagagcacac cggccagagt gatcgcccca cgcaccccgg cgaaagacgc
     1621 gatcaggatt tctcgtgtgg tccacgaacc aaactccatc ggcttcttct tcaggaagcg
     1681 gttgctgaac tttttcatcg tccacagcca gccgaaacgg accagcatca gcgccgcata
     1741 tatcagaata atatcggtaa acagcatcca gatttcgacg ttagggtcga tttctgccgc
     1801 catcagcgac gtctccagaa tacccggcag ctgcagacct aacagcagga acaccatgcc
     1861 gttaaagaca aattccagca tcgcccaggt gctgtttgcg cgcaggcgca ttgccagcgg
     1921 cgcacggcgc atcacaccgg agcgggtgat ggtcatcccg gcggcaaccg cagcgaggat
     1981 gccggaaacg ccaatatgtt cggcaatcag ataagaagcg aatggcagca agaacagcag
     2041 aacgatctgc gtcgcgggtt catcaccgcc ccagcggctg aggaatcgca gcgagcgacc
     2101 atacagccag ctcaccacaa agccggcgag aataccgcca atggctactt tcataaattc
     2161 gaccgtcgca ccgccaacgg tgaagatcat cgtccccatc gccactgcca cggcaaactt
     2221 caacgacacc aggccggagg cgtcattcat caacgcttcg ccctgcaaaa tacccatgat
     2281 ttttttcggg atgcgccctt cgccaacaat cccggagagc gccacagcat cggtcggaga
     2341 aagcactgcc gccagcgcaa aggcggggat cagcggaatg cccggcacca cccagtaaat
     2401 aaggaagcca atgccgacca cggtgaccac caccagcgcc agcgcgaggc caaaaatctc
     2461 tcgaccatgt tcgagaaatt cacgggtcgg cgttttccag ccatcagcga acagcaacgg
     2521 cgggataaac aagactaaaa agagttcagg atcaaactcc acatgcaaac caaacgtcgg
     2581 ccacgccagt agcgcaccga tggcgatttg cataagcgga agcgggatct gaaagggcat
     2641 gacacgagtg accaccccgg acagcgagac cacgagggtc attatcagta tggtgaagaa
     2701 gatttccatg cgttccctgt ttgcgatgtt tttttatgat ctgagaaaga cgttatgcct
     2761 gaattctcat tttttcagaa tatcgcaaaa gctcacacaa tgtgcggaga aaataaaaac
     2821 gggcagccat tgccacccgt tttcagaatc cgatgaaaga ttagatagcc cagccacccg
     2881 catagaaggt caccagcgcc acggcgataa cgaccgtacc gatgttcaac ttgcgccatt
     2941 cgccggaaac cagacgacca atcaccagag tcgcgaagcc gatcatgatg cctgttacga
     3001 tgttacaggt cagcacgatg aataccgccg taaccagacc cgccatcgca tcaacaaaat
     3061 cagcaaagtc gattttcgcc acgttgctca gcatcagcag gccaacgtac atcagcgccg
     3121 gagccgttgc ataccccgga acgaggtaag agagcggaga gaggaacaga atcagcagga
     3181 acagcacgcc aacggtgata gccgtcaaac cggttttacc gcccgccgcc gtacccgccg
     3241 cagactcgat gtataccgct gccggagcag cacccaccag gccagagaaa acgctgctca
     3301 tggagtcagt ggtcagtgct ttcccaccgt cgatgatctg cccatcttta tccagcaggt
     3361 tcgcctggcc ggcgacggca cggatagttc cggtggcatc aaatactgcc gtcatcacca
     3421 gcgccagaac gcttggcagg actacaggat tcagcgcgcc cataatgtcc aggctgccaa
     3481 tcagtgaatt gccgttttca tcgctcaatg aaggcatggc gaaaacgccg gagaaatgga
     3541 cgttaggatc gaagatcaaa ccgacaattg agataccgat aatggtcagc agaatgccac
     3601 cagggacttt cagtttttcc aggccgatga tcaccgccag acctactagt gacataatca
     3661 ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc atcaagcggg tttttaatca
     3721 ccagaccgac accattagcg gcaatgagca gcaggaacag accgataccg atccccgtgc
     3781 cgtgcgccac accgtgcggc aagttgcgca aaatccagct acggataccc gtggcagaaa
     3841 ttaccgtaaa cagcaccccc atcaggaaca cggcacccag cgcgacaggt acgctaatat
     3901 gttgccccag caccaggctg aatgcggtaa acgccgtcag ggagatggcg caaccaatcg
     3961 ccaacggcag attagcccac agacccatca cgatagaacc gagtccggca accagacagg
     4021 ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc caacatacct ggaacgacga
     4081 tgaccgagta gaccatcgcc agaaacgttg ttaacccggc aaccacttcc tgacggacag
     4141 tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga accgccggta cgcgctgatg
     4201 gcgtagacat aagaaaacat cccctgagag tttaattttc gtcaagatgc gtggcggaca
     4261 atcccctgcc agcgaaacgt tatcttcctg ctccaggttt gcgacaattt actgcgtcgc
     4321 aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg gtaaaaaaag caaacgatta
     4381 tccggcgatc atagcggctt tttcaaccga agtttgctgc ttttttctct tttattgtct
     4441 tgacgatgaa aaaccaggca attgatgcgc aaacgttatc gtcggtgcgc aagattgcag
     4501 cgttatcgag ccgtataccg ttcaggattg cgataaaaaa tcgtctgaga tgacaaaaat
     4561 attgcggata aaaagtatac ttttacactg gcgaagaaat gccggtgaca tacaattaaa
     4621 gcatcaacac caaccggaac ctccaccacg tgctcgaatg gggtgtgttg acgtcggggg
     4681 aaaccctcct gtgtaccagc gggatagaga gaaagacaaa gaccggaaaa caaactaaag
     4741 cgcccttgtg gcgctttagt tttgttcatc ttccagcaag cgtgcgccgg taccttcttc
     4801 tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg cgcgaaaggc agccacaacc
     4861 aatacatccg tccagttcgt cacgcagcgc tactaaggta tgaatgcgcc gatccaactc
     4921 ttctcgccat tgggacgaaa gctgtttcca ctctttcgca cttaacgtat gcccttcggg
     4981 caacacgcca aacgcttcac caatggtcgc cagcggaatg ccaatacgct gagcaatttt
     5041 gataattgca acatatcgca acacatcacg tttatatcgc cgctgattgc cgctgttacg
     5101 gatactggta atcaaccctt tactttcata gaaatgcagc gccgataccg ccacaccgct
     5161 gcgtttcgcc acttcgccgg gggttagcag cgctttaatg cggggtaatt tcttttccat
     5221 aaatcgcttt acctcaagtt aacttgagga attatactcc ccaacagatg aattaacgaa
     5281 ctgaacactg aaaagaggca gatttatgtc ccatcagaaa attattcagg atcttatcgc
     5341 atggattgac gagcatattg accagccgct taacattgat gtagtcgcaa aaaaatcagg
     5401 ctattcaaag tggtacttgc aacgaatgtt ccgcacggtg acgcatcaga cgcttggcga
     5461 ttacattcgc caacgtcgcc tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc
     5521 gatttttgat atcgcaatgg acctgggtta tgtctcgcag cagaccttct cccgcgtttt
     5581 ccgtcggcag tttgatcgca ctcccagcga ttatcgccac cgcctgtagt tttattgccc
     5641 gcgcgttaac tcccgcgcgg gtaattgctc catccattgc ataaacacct gcggcggcat
     5701 cgccttcgca aaaaaacatc cctggcaata gcgcacgccg cgtttgcgca gccagttaac
     5761 ctgctcctca gtttcgacgc cttcagcgat cgtttttaac cccaggctgt gcgccagctc
     5821 gatgatgtgt tccgcaatca aatgactggt tttgtgggtg gtcagcgttt caacaaacga
     5881 tttatcgatt ttcaaaatat cgacattcaa tgatttaagg ttatgcaagt tagagtagcc
     5941 aataccaaaa tcatcaattg ccacttcgta accagcctgg cggaaagcca gaataatcgg
     6001 cgtcattttg tcaacatcaa gaaatgcatg ttcagtcact tcaaatttaa tttgctgcgg
     6061 acgcaccgcg tattgctctg ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa
     6121 atcggaggcc gacaggttaa tagaaacata gcgatcggca tgtgttgcca agtacgcgcc
     6181 cagatcacgg aagacattat caataacata atcagttatc tgttctatca tcccctcctt
     6241 ttctgccagc ggaataaact ctgccggatt cattatttgc ccctgctcac caagccaacg
     6301 taacaacgct tcagcgccga tacatttttc tgttttgata tcgattattg gctggtaata
     6361 aagacaaagt tgatgttttt cgagggcgcg ttgcaattta cgtttgggag ataaatagtt
     6421 ttgtcgaata cgtagccaga gtaatagcag aacaagactc cccaaaatac cggcgggcaa
     6481 cgcaaaaata agatgattat aaaaatgggt tataagacgt tgatatgaag tcgcaacaat
     6541 ggctgcaatt gggcgttttg tcgaataaac tgtcgcatat aaatagccat ttctttgtac
     6601 ggttaaatcc ttcaaatgaa tcagcggaga aaacgttgct gccgaggcct ctttgcttaa
     6661 cgagaaaaag gttttcgtca ccgtatcata cacaccccat tgcaatgtcg gatcatcaga
     6721 catcacttca ctccagaaga gagggttgat aaccgccaca taatttcccc gctgcatata
     6781 ggtcatttta tagccagaga aaaaaggcgt atcgcggtaa taatagatag aaacgttagg
     6841 ttcacgctta taatcggccg gtgcaatcgt atagccgttt acaggcgcta tcagcgatga
     6901 gcataaaaaa tggttatcac gggcatagat caattcatta atataaagat agccacgaat
     6961 aatattcaac attcgctttt gatgggctgg agtgcaaact tgcccctgat agcgttcagc
     7021 tgcatcgctc actaaatctg cctggcgaat gaccagctcg gatttatcca gtgccagttg
     7081 agcaaaggtt cggagctgat tattcacttc tgattttgcc caaaggaagg caatccatag
     7141 cgaaagaatg atgggaaaga gaactaaaaa gataatgccc ggcaacgcca gtaattggtg
     7201 tcgtgcacga tgactcattg ctatcaattt cctgcctgtt tatacccggc cattgcaagg
     7261 tgaaaaccct gggtaccgac gtctttagat aaatcttacg gtataaatag cacttttgcc
     7321 tgatttgaaa ttgatacctt tctaatcgtc acctggatga aaataattca ttgatattca
     7381 aaattaattt cctacaggaa tgcagaggcg gcgggaagat atgcccttaa tgccactgaa
     7441 aaaccacata atctgtacag caataaaagt cacggcctaa tatggctaac aaacaatcaa
     7501 ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg tttcattttt gtgttctgca
     7561 agccccgcga tagccagagt gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc
     7621 atcggctctt ttcagaggaa ttgtttgatg gctaccctca ccactggcgt ggttcttctt
     7681 cgctggcaac ttcttagtgc cgtaatgatg tttctggcca gcacactcaa catccgtttt
     7741 cgtcggtctg attatgtcgg gcttgcggtg atcagcagcg gtctgggcgt tgtttccgcc
     7801 tgttggttcg caatggggtt gcttggcatc acaatggcgg acatcaccgc tatctggcac
     7861 aacatcgagt tggtgatgat agaagagatg aatcagacac cgccacaatg gccaatgatt
     7921 ttgacctgat acgtagaagc ctccataacg gaggcttctt ttttatggct ggcagatgct
     7981 ttaatcatcc accttaaaac aatataacct attgttttaa tgacaaatca gaacggaatg
     8041 tcatcatcaa agtccatcgg cggctcgtta gacggcgctg ccggagcgga ctgctgcggg
     8101 cgagactgcg cgccgccgct gaactgattg ccaccctgcg gctgctgtgg ctgaccccaa
     8161 ccgccctgcg gctgaccacc accgatattg ccacctgccg gagcgccacc accctgacga
     8221 ccacccagca tctgcatggt gccgccaacg ttcaccacga cttctgtggt gtagcgatcc
     8281 tgaccggatt gatcggtcca tttacgggta cgcagctgac cttcgatata aacctgagaa
     8341 cctttacgca gatattcgct cgccacttct gccagtttgc cgaacagcac aacgcggtgc
     8401 cattcagtct gttctttcat ctcgccggtc gctttatcac gccaggattc ggaagtagcc
     8461 agcgtaatgt tggcaactgc gccaccattt ggcatgtagc gtacttccgg gtcctgaccc
     8521 agattaccaa cgagaataac cttgtttacg cctctgctgg ccatgttcgt gtctcctgaa
     8581 aaaaatcgtt ctgaataagt gtaaacgcgc gattgtacca ttaccaatag cgcttttact
     8641 atgttgtgac ctcggttccg ggaaacaaac ctggccagac attgttacac aacactccgg
     8701 gtaatgcatt ccaatactgt atattcattc aggtcaattt gtgtcataat taaccgtttg
     8761 tgatcgccgg tagcaccatg ccaccgggca aaaaagcgtt taatccggga aaggtgaatg
     8821 gataagatcg aagttcgggg cgcccgcacc cataatctca aaaacatcaa cctcgttatc
     8881 ccccgcgaca agctcattgt cgtgaccggg ctttcgggtt ctggcaaatc ctcgctcgct
     8941 ttcgacacct tatatgccga agggcagcgc cgttacgttg aatccctttc cgcctacgcg
     9001 cggcagtttc tgtcactgat ggaaaagccg gacgtcgatc atattgaggg gctttctcct
     9061 gccatctcaa ttgagcagaa atcgacgtct cataacccgc gttctacggt ggggacaatc
     9121 accgaaatcc acgactattt gcgtttgttg ttcgcccgcg tcggcgaacc gcgctgtccg
     9181 gaccacgacg tcccgctggc ggcgcaaacc gtcagccaga tggtggataa cgtgctgtcg
     9241 cagccggaag gcaagcgtct gatgctgctc gcgccaatca ttaaagagcg caaaggcgaa
     9301 cacaccaaaa cgctggagaa cctggcaagc cagggttaca tccgtgctcg tattgatggc
     9361 gaagtctgcg atctttccga tccgccgaaa ctggaactgc aaaagaaaca taccattgaa
     9421 gtggtggttg atcgcttcaa ggtgcgtgac gatcttaccc aacgtcttgc ggagtcgttt
     9481 gaaaccgcgc tggagctttc cggtggtaca gcggtagtgg cggatatgga cgacccgaaa
     9541 gcggaagagc tgctgttctc cgccaacttc gcctgcccaa tttgcggcta cagtatgcgt
     9601 gaactggagc cgcgactgtt ttcgtttaac aacccggcag gtgcctgccc gacctgtgac
     9661 ggccttggcg tacagcaata tttcgatcct gaccgcgtga tccaaaaccc cgagctgtca
     9721 ctggctggcg gtgcgatccg tggctgggat cgccgcaact tctattactt ccagatgctg
     9781 aaatcgctgg cagatcacta taagttcgac gtcgaagcgc cgtggggcag cctgagcgcg
     9841 aacgtgcata aagtggtgtt gtacggttct ggcaaagaaa acattgaatt caaatacatg
     9901 aacgatcgtg gcgatacctc catccgtcgt catccgttcg aaggcgtgct gcacaatatg
     9961 gagcgccgtt ataaagagac agaatccagt gcggtacgtg aagaattagc caagtttatc
    10021 agcaatcgcc catgcgccag ctgcgaaggg acgcgtctgc gtcgggaagc gcgccacgtt
    10081 tatgtcgaga atacgccgct gcctgctatc tccgacatga gcatcggtca tgcgatggaa
    10141 ttcttcaaca atctcaaact cgctggtcaa cgggcgaaga ttgcggaaaa aatccttaaa
    10201 gagatcggcg atcgtttgaa attcctcgtt aacgtcggcc tgaattacct gacgctttcc
    10261 cgctcggcag aaacgctttc cggcggtgaa gcccagcgta tccgtctggc gagccagatt
    10321 ggtgcgggcc tggttggcgt tatgtacgtg ctggacgagc catctatcgg cctgcaccag
    10381 cgcgataacg agcgcctgtt gggtacgctt atccatctgc gcgatctcgg taataccgtg
    10441 attgtggtgg agcacgacga agacgcaatt cgcgccgctg accatgtgat cgacattggc
    10501 ccgggcgcag gtgttcacgg cggtgaagtg gtcgcagaag gtccgctgga agcgattatg
    10561 gcggtgccgg agtcgttgac cgggcagtac atgagcggca aacgcaagat tgaagtgccg
    10621 aagaaacgcg ttccggcgaa tccggaaaaa gtgctgaagc tgacaggcgc acgcggcaac
    10681 aacctgaagg acgtgacgct gacgctgccg gtgggtctgt ttacctgcat caccggggtt
    10741 tcaggttccg gtaaatcgac gctgattaac gacacactgt tcccgattgc ccaacgccag
    10801 ttgaatgggg cgaccatcgc cgaaccagca ccgtatcgcg atattcaggg gctggagcat
    10861 ttcgataaag tgatcgatat cgaccaaagc ccaattggtc gtactccacg ttctaacccg
    10921 gcgacctata ccggcgtgtt tacgcctgtg cgcgaactgt ttgcgggcgt accggaatcc
    10981 cgtgcgcgtg gttatacgcc aggacgtttc agctttaacg tccgtggcgg gcgctgcgaa
    11041 gcctgtcagg gcgacggtgt gatcaaagta gagatgcact tcctgccgga tatctacgtg
    11101 ccgtgcgatc agtgtaaagg taaacgctat aaccgtgaaa cgctggaaat taagtacaaa
    11161 ggcaaaacca tccacgaagt gctggatatg accatcgaag aggcgcgtga gttctttgat
    11221 gccgtacctg cactggcgcg taagctgcaa acgttgatgg acgttggcct gacgtacatt
    11281 cgcctggggc agtccgcaac cacactttct ggtggtgaag cccagcgcgt gaagctggcg
    11341 cgtgagctgt caaaacgcgg caccgggcag acgctgtata ttctcgacga gccgaccacc
    11401 ggtctgcact tcgccgatat tcagcaactg ctcgacgtac tgcataaact gcgcgatcag
    11461 ggcaacacca ttgtggtgat tgagcacaat ctcgacgtga tcaaaaccgc tgactggatt
    11521 gtcgacctgg gaccagaagg cggcagtggc ggcggcgaaa tcctcgtctc cggtacgcca
    11581 gaaaccgtcg cggagtgcga agcttcgcat acggcacgct tcctcaagcc gatgctgtaa
    11641 tcgttaaggc cgctttctga gcggcctttt cctttcagag ttgtaccagc aatttacgct
    11701 tttcttccgg tagtaaattc actgcctgct gataagacgc atccaccaga taatagattt
    11761 gcgaatccgg cagcgaaccg tcgagataca cggtgctcca gtgcgcttta ttcagatggc
    11821 ggctaggacg cacatcgctg tgctgctgac gtagcagctc cgccagctcc gggctggttt
    11881 tcagcgaaac agctgggcga ttttctactt ctttcaccat cgcaaacagt acatcttcca
    11941 ctttgatctg cgtcgctttc cagtcgttat gcacgctctg ttctgcgcct ggttttgcca
    12001 tgcaatattg tagcaactcc gaaatggtca ttttttactc cccttgtagt gtcgcgatga
    12061 tgcgacgcga tccgccgtgg atgcgatgtt cccccagcca gatgccttgc caggtgccgg
    12121 tctgaatacg ccctttatgt accggcaata caagcgatgt tcccagcatt gaggatttga
    12181 tatgagaagg catatcgtct gctccctcat agtcatgctc ataatttccg ttgtcgggaa
    12241 cggtgcggag gaaaaaacgc tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa
    12301 gtgtcagaga ggcggaggta tgttgcagca acagatgcag taagccgatg ttaacgcgcg
    12361 gcatatcagc cagctgattc agaatttcat ccgttaccag atgaaaccca cgagatttgg
    12421 cgctaagcgt gagcgtcttt tgataccaca tgtgctgctc cttgataaaa ctctcttaat
    12481 cagtttgcag caagacagcg aaaggataaa ggtgtgatta aaaaaacagc attcgggaga
    12541 gcgtcacgct ctcccgctct gtcagtattc tgaattgacg atcacctctt caccaaacgc
    12601 acccgcttgt ggcaagggtt tgtaggtaga gttggaggcg cgcagaatgc ggataccacg
    12661 agcgccgaca tcgcgtgcgg cggtaatatc attatcagaa tcgccataaa aaattcggat
    12721 atttttatcc tgcagccatt gcgattttgt attttgccct agtttatcgc ccgcaaagat
    12781 caccggattc atgttggtgg caggaatatg aaaattatcc gccagcgttt ttgaaaccgt
    12841 ttctgttttc gtcggactac gaccagtcac aaagaagatc gcgtcaccgc ggcgtacatg
    12901 catatcaatc agctggcgag cgacctcttt tggaatgctg aattcatccc agccattgtt
    12961 cattttttcc cagaacacag gatttttcag ataatcttcg ctttctggcg agaaggtttt
    13021 tttgccgcgc cagaagcccg gactggaaaa aagtaccgtg tcatcgatat caaaccccac
    13081 cgccattggc ggacgccctg cgaggctatt ttcaatttgt gcgaccgaaa cccaatgaat
    13141 gggtgcctgt tcagcaagcc tggcaacgtt agtcccaggg ttaagcggtg aaggagatga
    13201 ggccagggca acagcggaac tgtttagagc gaacaataag caaacggcac tgattgcctg
    13261 tgtgatcttg cgcatatttt tccctaaata gtcagtatgt tgaaactttt tattgtgaga
    13321 tttgttgcaa aaaccatctg accataacgc cagctgccgg ggaaaggaag gattatctgc
    13381 ggtttttgtg atgaaaagca ggatgattat atttaaccca gtaaatacgt tcctgtcttt
    13441 ttttgtataa aaaatgttta ttactcgatg taaggataca ttaattttac gtttattatg
    13501 atgaatcctc ggcaaatgag cgaaatttga tagaacattt atctgctgta cgatttaatg
    13561 aacttaatga tacagcatta caaaaacaat cgaagtttat aaagatgatt tctgattgac
    13621 cagcccccta ccctacaatg gtgattaatt ttattactcg cgatggagat gatgatatga
    13681 atatatcata tgtgaatagc aacaaaacga catctttacc tgttgagcta gatgcactaa
    13741 ataataaaga tatctcttat gccaaagatt tttcttatgc caaagatttc tttttataca
    13801 tagaaacaca gttaaaaatt gcaaaagatt tttgtagacc tggagaagaa gtatcaagtt
    13861 ctattgcaag taaagttttt cacgcattta ttgatttagt taacaaaatc aggggtaaga
    13921 aagactttat gtatatttgc acgctttgct gttttgccga ggaggttaaa ggtgattatt
    13981 ctcattacag gaccttttta tttgatattg gtaatcaata taaggttaaa cttacacaaa
    14041 gcggaaaaaa agagctctct ttaactttag aatttaacga tactataatt gaatctcaga
    14101 aagtcacagg caataaggca aagcatattc ttgaggatat agaaatattc tatcgtaaca
    14161 aacccgatac ttattattaa atttaataat ggttagatgt gcagaaatta ttactacacc
    14221 caaaaaaaca ggataaggaa ttccagataa ttgggataag cttaacaata aaagaactaa
    14281 caacgtttat tatctctcac gtagaacgat ggcatcaaaa aaatcatcta taaagaaaga
    14341 gtgaaagcga cgacagttta atttcactgc aggctgggta gctccagcct gctttcctgc
    14401 attacatcac cgcagcaaac gcctttgcca cacgttgtac atttgccgta tttaacccgg
    14461 cgacacacat gcgaccgctg gcgatgagat agacaccaaa ttcttcacgt agtcggtcaa
    14521 cctgagcggc acttaaaccg gtataactga acatgccgcg ctgattaagc agataatcga
    14581 aattgcgttc tggcatctct gtgcttaata ccttcaccag ttcctgacgc attgccagaa
    14641 tgcgagtacg catctcttct acttccgcca gccagctggc tttcaatgcc tcgtcattca
    14701 gcactgcagc caccacctgc gcaccaaaat tcggcgggct ggagtagttg cggcgaactg
    14761 ttgctttcaa ttgccccagt acgcggccag cggcttcggc atcttcacac agaacagaaa
    14821 gtccgccgac gcgctcgccg taaagggaga aaattttcga gaacgaattg ctcaccagag
    14881 cgggtaatcc agcgctggca atggcgcgaa tagcgtaggc atcctcttcc ataccggcac
    14941 caaatccttg ataggcaata tcgaggaatg gaataagctc gcgggatttg agaatttcaa
    15001 tcaccgcatc ccactgatca ttagtgagat cggcacccgt tgggttgtgg caacatggat
    15061 gcagcaacac aatactgcgg gcaggtaatg ttttcagcgt cgccaacagg tcattaaagc
    15121 gcacgccgtt agtcgcttcg tcataccagg ggtaagtact cacttcgaat ccagccccgg
    15181 cgaatattgc tacgtggttt tcccaggtag gatcgctgac ccagacgcct gattccggga
    15241 agtagcgttt caggaaatcc gcgcccactt tcaatgcccc tgagccgcca agggtttgaa
    15301 tggttgctac gcgctgttgt tgcagtactg gatggtccgc accaaacagc agcggcgcaa
    15361 tggcatggcg atagctgtta agcccttcca tcggtaaata aagcgaagcg ccatgaggct
    15421 gcgcattcag gcgcgcttcc gcctccgcca cggcttgcag ttgtggaata attccgtctt
    15481 cgttgtagta cagaccgata cttaaattca ctttgtcgct gcgagggtct tctttaaaac
    15541 gctccataag cgtaagaatc gggtcgccag cgtaggcgtc aactttttga aacacgcgat
    15601 ggttctccag gtttacgggc aggtggttaa aacacaataa accggaagaa ggcgaagatc
    15661 gagtggatgt tcaggagcga acggcaatta gcaacagagt gagactcatg acaaacgtac
    15721 atccgccaga ggcacgacct ttataagagc atgaaagaac atcaacttat tgaattttta
    15781 ggattttatt ggccggataa ggcattcacg ccgcatccgg cacagacaat caaatattac
    15841 agaacgataa atccacgtat ttcatcgcga cccttgaagt caggcgcgta ataagttcgt
    15901 aagcgcttac tttcgtcatt tcagcgatac gttctacggg caaaccttcg ccccataaaa
    15961 tgaccggatc cccggctttg tcctgcgctt gtggacctaa gtctacgcag atcatatcca
    16021 tcgccacgcg cccgacaatc ggtacttcgc gaccgttcac cagcactggc gtaccggacg
    16081 gcgcggcgcg cggataacca tcgccatagc ccatcgcgac tacgccaaga cgggtatcac
    16141 gttcgcttac ccaggttcca ccataaccaa caggttctcc ggctttatgc tcacgcacgg
    16201 caatcaggct ggaggttagt gacatcactg gctgacagcc aaaatcggca ccggtggagc
    16261 gatcttccag cggcgagacg ccataaagaa tgatgcccgg gcgcacccag tcaaaatgcg
    16321 actgtggcca cagcagaatg ccacccgatg cggcaatgga acgttgacca ggtttgcctt
    16381 cgcaaaaggt attaaagata gcgagttgtt tctcggttgc gccacatttt ggttcatccg
    16441 cgcgcgcaaa atggctgacg atattcaccg gctgacgaac gtttttgcac tgggtcaggc
    16501 gatgataaaa cgcctcagcc tgttccggcc ttacgcccag acggtgcata ccggtatcga
    16561 gtttcatcca gacggtaacc ggctcgtcca ggctagcctc ttccagcgca gccagctgtt
    16621 cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga tctctggcat
    16681 caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc agtcgcagag
    16741 cttcttcgag acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa
    16801 gaagaccgtg accataagcg ttcgctttca ccaccgcaac cattttactg gcaggggcca
    16861 gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg
    16921 cttgcatttg tgttccttga taagtgtttg ctttaattac ctaattcata aaataattat
    16981 tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg accgttaaag
    17041 gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac cgataataat ttccgcgatg
    17101 ccttttaaat cactgttttc gtgatacacc tcatcacgat agataaacat gatcaagtcc
    17161 gcatcctgct cgatagagcc agattcacgc aggtcggagt tgaccgggcg tttgtcggca
    17221 cgttgttcca gagaacggtt caactgggac agcgccacca ccggcacgtt cagttctttc
    17281 gccagtgctt tcagcgagcg ggagatttct gcaatttcca gcgtacggtt atcggaaagc
    17341 gccggtacgc gcatcagttg caggtagtcg atcatgataa gcccgatgcc gccgtgttca
    17401 cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga ggagtcatcg
    17461 atatagatat tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg cgcccagtct
    17521 tcatcatcga gctgcccggt acggatttta gtctggtcaa cgcgcgacag cgacgccaga
    17581 gaacgcatca taatctgttc tgaaggcatc tccagcgaga agataagtac tggtttatcc
    17641 tgcaacatcg ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc catcgacgga
    17701 cgcgcggcga cgatgatcaa atccgacggc tgcaagccag cggttttttt gttgagatcg
    17761 tcataaccgg tgtttactcc ggtaacgcca tcgtgtggct gctgaaacaa ctgctcaata
    17821 cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc tttgtttgca
    17881 cgactttcgg caattttaaa gacgcgggat tcagcaaggt ccagcagatc ttcgctggta
    17941 cgcccctgcg gatcaaaacc ggcttcagca atctcattcg caaccgagat catctcacgg
    18001 acaacggcac gttcacgcac gatgtcagca taagcactga tgttcgccgc acttggcgta
    18061 ttttttgaca gctctgccag ataagcaaaa ccaccgacgc tatcgagttg cccctggcgt
    18121 tccagcgatt ccgcaagggt aatcagatcg ataggactac cgctttcctg caaacgcgcc
    18181 atttcagtaa agatatggcg gtgtgggcgg gtgtaaaaat cgtctgccac cacacgctcg
    18241 gctacatcgt cccagcgttc gttatccagc attaaaccgc ccaacaccga ctgctccgct
    18301 tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc gcggggttca
    18361 gcctgctgtt tgttgaaggg tttatttcct gccatagtga atggagttac cgagataaag
    18421 aatgggtcga aactttacca tatgaagcag cccctgacga tacgttctgg aggacacatg
    18481 gcaacacgaa ttgaatttca caagcacggt ggcccggaag tacttcaagc cgtagagttc
    18541 actcctgccg atccggcaga gaatgaaatc caggtcgaaa ataaagccat cggcatcaat
    18601 tttatcgaca cgtatatccg cagcggcctt tacccgccgc catcactacc cagcggatta
    18661 ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca tattaaggca
    18721 ggcgatcgtg ttgtctatgc gcagtcggcg ttaggcgctt acagctctgt gcataacatt
    18781 aatgcggata aagcggcgat tctgcctgcg gcaatttctt ttgagcaagc tgcggcatcc
    18841 ttcctgaaag gcttaacggt ttattatctg ctgcgtaaaa cctatgaaat taaacccgat
    18901 gaacagttcc tgttccacgc agcggctggc ggcgttggct tgattgcctg ccagtgggca
    18961 aaagccctgg gcgcgaaact tatcggcacc gtaggaaccg cgcaaaaagc gcagagcgcg
    19021 ctaaaagcgg gcgcgtggca ggtaattaac tatcgtgaag agaatctggt cgagcggtta
    19081 aaagagatca ccggcggtaa gaaagtgcgc gtggtgtacg attccgtggg cagagacacc
    19141 tgggaacggt cgctggattg cctgcaacgc cgcggcttaa tggtcagttt tggcaactca
    19201 tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc aaaaaggctc gttgtatgtg
    19261 acacgccctt ccctgcaagg ctatatcacc acgcgggagg aattaaccga ggccagtaat
    19321 gaactgttct ctttgattgc cagcggtgtg attaaggtcg atgtcgccga gcagcagaaa
    19381 tatccgctga aggatgcgca gcgtgcgcat gagattctgg aaagccgggc gacgcaaggt
    19441 tccagcctgc tgattccata aaagaaatag ggcttccacc tgggaagccc tttcttttta
    19501 tagttcggct gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa tagtgacaga
    19561 tttgattatc aattcctatt ttgttctaag gataaaacct taggttgtga tcatccgcac
    19621 aatcccttag taacgccagc ggtcataacg ctgatattta ggcacttttg gtgctttaat
    19681 cgccttaata acccacacca ccgcaatcgc cagtagtaac cacggcagca acttaatcat
    19741 caatgccagc ataccgccga ggaacataat ggccgtcgcc acaaccagcg cggcgataat
    19801 gcccagcaac gaaacgccgg tgaccatcag catgacaaaa aagccaatca caaaaagtag
    19861 ttccagcatg atgctctccc aaatatgaaa tctcttgctg gcattacaag aatcatgcca
    19921 aaaataatct attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg cgtggtgaat
    19981 ttgactactt tttggtgaaa agttaacgct tatccgccac cagtttgagc gcgtgttcca
    20041 gcacattaat gtctgcaccc gctttatggg cattttcact taaataacgc cgccactgcc
    20101 gcgcgccagg aataccctgg aacaagccca acatatgccg ggtaatatgg ccgagatacg
    20161 tcccctggct gagttcacgc tcaatgtacg gatacatggc gcgcactacc gccaccggat
    20221 cggcatcggt atccgaggaa ccaaagatct cccggtctac cgccgccaga atacccggat
    20281 tctgatacgc ctcgcgcccg accatcacgc catccatatg ttgcaggtgt gctttggcct
    20341 cttccagcga cttgatacca ccgttaatcg acattgtcag atgcggaaag tcacgcttca
    20401 gttgatacac acgcggataa tcgagcggcg ggatttcacg gttttctttc ggacttaacc
    20461 ccgaaagcca ggctttacgt gcgtggatga taaacatctc acactcgcct ttgccggaaa
    20521 cggtgttgat gaaatcgcag agaaattcat agctgtcctg gtcatcgatg ccaatacgcg
    20581 ttttcaccgt cactggaatc gacaccacat cgcgcatcgc tttcacgcag tcggcaacca
    20641 gctgcgtatt acccatcaga cacgcaccaa acatgccgtt ctgcacccgg tcagacgggc
    20701 agccgacatt caggttgatc tcatcatatc cacgcgcttc tgccagcttc gcacactgtg
    20761 ccagcgccgc cggatcgcta ccgccgagtt gcaacgctac cggatgttct tcttcactgt
    20821 acgccaggta atcaccttta ccgtgaataa tcgcccctgt ggtcaccatt tcggtataca
    20881 gcaacgtatt gcgggaaagc agacgcaaga aatagcggca atgtctgtcc gtccagtcga
    20941 gcattggtgc aacgctaaac cgaccactcc agtgaacgtc agttttttca ggcattgcgc
    21001 tggtttggtt gattttttgc atttcagaat taccgtgcat tttcaaatgt agagattatt
    21061 ttatcgatat atcatggggt tatgttattc agcatcacta ttcaggaggc tcaatagcgg
    21121 ggtactatac cataacaaca ggaagcgcct gtctcattgc aaaagaaaat tgagatcctc
    21181 tcaaggcatg aagctctcac gaagtgatgg aaataatctt attagccgtt aactttgtta
    21241 aggccaatga taaacaatcc aggttcgacg ataaataaaa aatcacacat taaactctgg
    21301 tgatatatct ccctgctaat agcattgata gagaaaaaag aacccaataa atattgggtc
    21361 cttttatata atgccttcca tactatcgaa gaacttcaca tattatttct ccgatttaac
    21421 cccgaacaaa tcataaatta atttagaagt gtctgtaagt attttaatct cttcctttga
    21481 ggttgggtca aacgacttcg cgaagctaat taattgtggt gcagcatctc gcattttgct
    21541 taaaatatca ggatcgagcg ttccttcatt caccagatgt gacatcttat ccagatagcc
    21601 atcaaaattc atttctcccc catcagccag acgcttcatc tctcccaggt acttcttcat
    21661 atcacgttga gataattttt caaactgagc tttcaggtag ctctcattat tttcattaat
    21721 atacactgtt tctgataaat ctgcgtaggc actagaatat gaaagtgaaa ttaacagcga
    21781 agcaaataat tttaagctgt tgtcatcatg ttcctggcag gcattaacaa acgttaaaaa
    21841 accagaacct attgcttgaa aatgtatatt cgctaaaggt tcattatcct tagattcctc
    21901 ataaaatagc tgaaccgtgg agggaaggtc attcttattt tctacatcaa aagtacattg
    21961 ttcaatgggc attgcatctt tattatcccc aagattacat atactattaa tcgcgaaatg
    22021 aaaaatagcc cggtcaacaa ctgatgcaat aaagtcacga tcgtttaaat cctcatcagt
    22081 gcaatcgtca atatacttat tgacccacat tttgagatca tttatttgct caattaaaaa
    22141 cacatttttt tacgtgcatg ccataaatta tgcgtgattc gttgaataaa caaatctatt
    22201 tttgcaataa ttatatcccg ataacgcagc gctgcat
//
LOCUS       DAZFKM010000079        21752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFKM010000079 DAZFKM010000000
VERSION     DAZFKM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..434)
                     /locus_tag="ACHKAY_003102"
     CDS             complement(<1..434)
                     /locus_tag="ACHKAY_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO5701745.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGG"
     gene            complement(637..1038)
                     /gene="nikR"
                     /locus_tag="ACHKAY_003103"
     CDS             complement(637..1038)
                     /gene="nikR"
                     /locus_tag="ACHKAY_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO5701746.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1044..1850)
                     /gene="nikE"
                     /locus_tag="ACHKAY_003104"
     CDS             complement(1044..1850)
                     /gene="nikE"
                     /locus_tag="ACHKAY_003104"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO5701747.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1847..2611)
                     /gene="nikD"
                     /locus_tag="ACHKAY_003105"
     CDS             complement(1847..2611)
                     /gene="nikD"
                     /locus_tag="ACHKAY_003105"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO5701748.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2611..3444)
                     /gene="nikC"
                     /locus_tag="ACHKAY_003106"
     CDS             complement(2611..3444)
                     /gene="nikC"
                     /locus_tag="ACHKAY_003106"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO5701749.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3441..4385)
                     /gene="nikB"
                     /locus_tag="ACHKAY_003107"
     CDS             complement(3441..4385)
                     /gene="nikB"
                     /locus_tag="ACHKAY_003107"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO5701750.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4385..5959)
                     /gene="nikA"
                     /locus_tag="ACHKAY_003108"
     CDS             complement(4385..5959)
                     /gene="nikA"
                     /locus_tag="ACHKAY_003108"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5701751.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6070..6657)
                     /gene="acpT"
                     /locus_tag="ACHKAY_003109"
     CDS             complement(6070..6657)
                     /gene="acpT"
                     /locus_tag="ACHKAY_003109"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO5701752.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6712..7761)
                     /locus_tag="ACHKAY_003110"
     CDS             complement(6712..7761)
                     /locus_tag="ACHKAY_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO5701753.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7893..9110
                     /locus_tag="ACHKAY_003111"
     CDS             7893..9110
                     /locus_tag="ACHKAY_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5701754.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9114..9671)
                     /gene="dcrB"
                     /locus_tag="ACHKAY_003112"
     CDS             complement(9114..9671)
                     /gene="dcrB"
                     /locus_tag="ACHKAY_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO5701755.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9744..10409)
                     /locus_tag="ACHKAY_003113"
     CDS             complement(9744..10409)
                     /locus_tag="ACHKAY_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO5701756.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10630..10875
                     /gene="tusA"
                     /locus_tag="ACHKAY_003114"
     CDS             10630..10875
                     /gene="tusA"
                     /locus_tag="ACHKAY_003114"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO5701757.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10977..13175)
                     /gene="zntA"
                     /locus_tag="ACHKAY_003115"
     CDS             complement(10977..13175)
                     /gene="zntA"
                     /locus_tag="ACHKAY_003115"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO5701758.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQRVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13249..13875)
                     /locus_tag="ACHKAY_003116"
     CDS             complement(13249..13875)
                     /locus_tag="ACHKAY_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO5701759.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14016..14375
                     /locus_tag="ACHKAY_003117"
     CDS             14016..14375
                     /locus_tag="ACHKAY_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO5701760.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14378..14647)
                     /locus_tag="ACHKAY_003118"
     CDS             complement(14378..14647)
                     /locus_tag="ACHKAY_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO5701761.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14637..15233)
                     /gene="rsmD"
                     /locus_tag="ACHKAY_003119"
     CDS             complement(14637..15233)
                     /gene="rsmD"
                     /locus_tag="ACHKAY_003119"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO5701762.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15383..16879
                     /gene="ftsY"
                     /locus_tag="ACHKAY_003120"
     CDS             15383..16879
                     /gene="ftsY"
                     /locus_tag="ACHKAY_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO5701763.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16882..17550
                     /gene="ftsE"
                     /locus_tag="ACHKAY_003121"
     CDS             16882..17550
                     /gene="ftsE"
                     /locus_tag="ACHKAY_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO5701764.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17543..18601
                     /gene="ftsX"
                     /locus_tag="ACHKAY_003122"
     CDS             17543..18601
                     /gene="ftsX"
                     /locus_tag="ACHKAY_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO5701765.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18846..19700
                     /gene="rpoH"
                     /locus_tag="ACHKAY_003123"
     CDS             18846..19700
                     /gene="rpoH"
                     /locus_tag="ACHKAY_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO5701766.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19971..21074
                     /gene="livJ"
                     /locus_tag="ACHKAY_003124"
     CDS             19971..21074
                     /gene="livJ"
                     /locus_tag="ACHKAY_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO5701767.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(21273..21656)
                     /gene="panM"
                     /locus_tag="ACHKAY_003125"
     CDS             complement(21273..21656)
                     /gene="panM"
                     /locus_tag="ACHKAY_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO5701768.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5159 a         5741 c         6029 g         4823 t
ORIGIN      
        1 ccgccgcgca ccagccagag tgattcgctg cggctgtaac cgtcctcacc gggaaacagg
       61 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      121 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      181 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      241 gcgatgtcgg tttcaccggg aaggaccttt gcacccagca gcggattgac cggatggccg
      301 gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc aatacgtacc
      361 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcaccctg acgcgccgcc
      421 ggttttccgc tcatcgtaaa atccttttct attattttaa tgacagtatc ttcatacgaa
      481 aaagcgaaga aaaacttgtg gtcacaatga attgaagatt tttaggaata tatttagaac
      541 gttacaaaag agaaggttcg gatagggtat ttagagagga atcaggtgtg ctagtggccc
      601 tttgttgatc gttcaccaaa ggacccaata atagactcaa tcttccttcg gcaagcactg
      661 caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
      721 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
      781 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
      841 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
      901 ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt cggaacgatt
      961 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     1021 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     1081 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     1141 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     1201 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     1261 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     1321 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     1381 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     1441 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     1501 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     1561 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
     1621 gccagcggtt cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc
     1681 aaccgcgcga gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg
     1741 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat
     1801 ccaccgtgcg catagtgatg ggaaaggccg gagatgttaa gtaaagtcat gatgccagct
     1861 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcactgta tgtttggggg
     1921 cgttaaacag cgtttctaca tcgccctgtt cgacaatctt accgtcagac atcaccgcca
     1981 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt caccagcagc attcccggcg
     2041 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
     2101 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
     2161 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
     2221 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
     2281 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg
     2341 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa
     2401 ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
     2461 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
     2521 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
     2581 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
     2641 gtccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
     2701 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
     2761 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
     2821 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
     2881 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
     2941 cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata
     3001 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
     3061 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
     3121 catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc
     3181 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgt
     3241 catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc
     3301 cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg
     3361 gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
     3421 gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
     3481 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
     3541 agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
     3601 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg
     3661 atatgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat gtggcgacgt
     3721 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg
     3781 acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg
     3841 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
     3901 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
     3961 ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc
     4021 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
     4081 agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag
     4141 ataccaaagt caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc
     4201 tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
     4261 gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
     4321 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
     4381 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
     4441 aggggatgtt acccagctcc ggttttgata ccaccatcat tgagatgtaa ctgataggca
     4501 gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg
     4561 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggt ttgtcggcta
     4621 atccttgctg tgcctggaag tcagcgtgtg acggtacgcg cattgaactg aggaaggcgt
     4681 gtggatcata tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
     4741 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
     4801 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
     4861 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
     4921 tccaaccggc tttttccagc aacgctttcg ctttctgcgg atcgtactgg cgcggtttca
     4981 ggccgaggtt ggcgtagggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
     5041 catacaacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
     5101 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
     5161 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
     5221 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
     5281 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
     5341 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
     5401 acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
     5461 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
     5521 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
     5581 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
     5641 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
     5701 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
     5761 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
     5821 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtggtgattt
     5881 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
     5941 ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat
     6001 gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
     6061 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
     6121 agtaggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga ctgacagcga
     6181 ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
     6241 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
     6301 ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc agtgccagtt
     6361 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac tcaacagcag
     6421 ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcatttccg gcgcaaatgc
     6481 aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
     6541 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
     6601 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
     6661 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
     6721 taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt
     6781 ggtttccatc cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg
     6841 gccgagcagc catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag
     6901 gcgatggccc atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa
     6961 caatgcgccg accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg
     7021 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac
     7081 tgcccacatc agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc
     7141 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
     7201 gtgtaatcct gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac
     7261 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
     7321 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
     7381 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
     7441 tttaaaaagt ttgcgcgtca actccttatt aaacttcggc agcatagaga taaattcgtt
     7501 aaacgatgcc gccagtacgc cgaccagcgc agttagtgcg atcagcatca ccaccaccac
     7561 aatcgtaatg gcaatggggc gttgtactcc ccgacgaata aaccaggtga cgagcgggtt
     7621 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
     7681 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
     7741 atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
     7801 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa
     7861 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
     7921 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
     7981 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
     8041 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
     8101 gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt
     8161 tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc
     8221 ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt
     8281 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
     8341 gagtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag
     8401 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
     8461 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
     8521 cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggcg ctggcactgg
     8581 cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
     8641 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
     8701 tattccctaa cggcattaac cgtatcggtg gcttaaacgt agcgatgatt tgctttagcg
     8761 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg
     8821 gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt gtagtggcgg
     8881 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
     8941 atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
     9001 cggtgattta tctggcggcg gcgggactgg tcacaatcgc gttattactg acgtggcgat
     9061 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
     9121 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct
     9181 ggcagagtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
     9241 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
     9301 agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct
     9361 tccagacgct tcgccagcac cgccagatct tcttttggat catcgcccat gatgacgatg
     9421 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
     9481 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
     9541 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
     9601 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
     9661 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
     9721 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
     9781 gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata
     9841 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
     9901 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta
     9961 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactttgacg caggcggtta
    10021 aaaacgtgca cgtcgaggat ttgccccagc gcgtaggcca tgaaactggc ggtggcgata
    10081 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga acccatatag
    10141 aacagcgacg agatgacata ggagattaat aacgcaggga tcattaccgc gaagataatg
    10201 cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag aaaaataaac
    10261 ggaaagctaa acgcgcccca ggtggtatgg aaaccaaaaa tggagacggg aagctgaacc
    10321 agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta
    10381 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    10441 gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt
    10501 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    10561 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat
    10621 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    10681 cgctgcccgg aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa
    10741 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    10801 tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt
    10861 cgtaaaggcg gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac
    10921 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    10981 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    11041 agcaccgcca gccatagtcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc
    11101 agtcccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    11161 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcggc ggtttccagc
    11221 gccacgtctg tgccgctgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    11281 tcgttaatac catcaccgac catcgccagc ggcgcatgtt gattcagctt ggtcaccgct
    11341 ttgactttct cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    11401 gccgccgctg cgcgtggatt atcgccggtg aggatcactc ctttaactcc cagcgcgttc
    11461 agttcactga tggcagttgc agcgtcggcg cgcagggtat cctgcaatgc aatgataccc
    11521 agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta
    11581 atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca
    11641 ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga
    11701 atggcgagtt cagcaacctg tgcttctcgc acgatggcct gcgccagtgg atgcgtcgca
    11761 ccttgctcga ccgccgctgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    11821 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    11881 tgggtaacac gacctagttg ttccagcgcc gctccgcctt taattaacgc cccacgacgc
    11941 gctgctgccg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg
    12001 cagccaatca gcagcagcgt caggccttta taaatccact cttgccagct ggcggcaaac
    12061 agcagcggtg gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata
    12121 cggctgaaac ggtcgataaa ccgctcaatg ggtgcgcgac gctcttcggc ttcttcgatc
    12181 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    12241 agacggtcta cgctggtggc tccagcaggg actttatcgc ccgttgcgcg ctccaccgga
    12301 atagattcgc cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg
    12361 tcggcaggca aacgtccacc tgcggcgact tcaatcacat cgccagggcg caggctgtta
    12421 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    12481 attaacgcgc tgaccctttg acgtgcgcgg ctggcggccc agccttccag tcgttcgcca
    12541 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa aagcgcacca
    12601 atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcaaccgt
    12661 aatgcctgac gagcaatcgg gtacagccca accagtgtgg tcgcgataaa cgccagttgc
    12721 ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac gattagcgta
    12781 atcagcggca gattctcttt caggcgtgat gcttgcggtt cgtcggcgga ctgttcatcg
    12841 cgcagggagt agcccgcttt ttgcaccgca gattcaacct gggcacggat gtcattgtcg
    12901 gcatcaacca ccagtttttc ggtggcgaac aacacctgta cctgattcac gcctgcaagc
    12961 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc gctgactttc
    13021 cagctatagc gggtgccgga gacgttttca gagagagttg gcgtgctgga acatgcgccg
    13081 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    13141 ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta agttttttct
    13201 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    13261 cgagcggacg atcaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    13321 acggaagcgt cggcgatagt gactacccag ccagacaaag ttactgataa acagcaacga
    13381 agcgccgaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    13441 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    13501 cagccgcgtc cagataatcg ccagtaacag cgcgcccaga accagcagca ccagtggcag
    13561 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    13621 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    13681 gcccagcagt gaggcgcaca gccccgccag caccagatag ctaatggcgt cgaacatcgg
    13741 cgcttgccag gccagtaaca gcaggagaag aagggttaat ggtttaaata cccagcgttg
    13801 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    13861 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    13921 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    13981 atgcttaatt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    14041 ttgttatcat tggcttaatc atcgtcgccg catcgtttcg ttttatgcaa caacgacggg
    14101 aaaaagctga taatgatatg gcaccactcc agcaaaagct ggtgttggtg agcaacaagc
    14161 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    14221 gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    14281 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    14341 gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct
    14401 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg
    14461 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca
    14521 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcggacgt gggaagggat
    14581 gcaccaggtt gaggattaaa aatccccaaa cacagagcat taacaaacga ccaatattaa
    14641 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    14701 actttttccc gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg
    14761 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    14821 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta
    14881 cctttttgcg ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    14941 agtgtcgcca gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtt
    15001 gcccccgcag catagcgcga caacgcttcc agccccagcg cgccgcttcc ggcgaagcaa
    15061 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    15121 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    15181 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    15241 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    15301 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    15361 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    15421 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc
    15481 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga
    15541 agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga
    15601 agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca
    15661 gccggaaccg gtcgcagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc
    15721 gttgccagaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    15781 aaccgtagag attgtcgaag cggcagaaga agaagcggct aaagaagaaa tcaccgacga
    15841 agagctggaa gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtagttcc
    15901 tccggcagaa gaagagcagc cggtggaaga aattgcccag gagcaagaaa aaccgaccaa
    15961 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    16021 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    16081 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    16141 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    16201 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    16261 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa
    16321 gctggcacgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    16381 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    16441 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    16501 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    16561 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    16621 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    16681 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    16741 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    16801 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    16861 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    16921 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    16981 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    17041 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    17101 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    17161 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    17221 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    17281 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    17341 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    17401 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    17461 acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct gagcgatggt
    17521 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    17581 tggcgggcgt cttgatcgct tccgtaaatc ggtcggcggc tctggcgacg gcggtcgtaa
    17641 cgccccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgtaaaacca acgttttcaa
    17701 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    17761 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    17821 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    17881 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    17941 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    18001 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    18061 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    18121 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    18181 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    18241 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    18301 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    18361 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    18421 tctgatgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt
    18481 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    18541 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    18601 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    18661 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    18721 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    18781 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    18841 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    18901 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    18961 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    19021 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    19081 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    19141 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    19201 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    19261 tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    19321 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    19381 acatgacctt tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    19441 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    19501 agcaggcggc aaaccgtctg accgacgcga tgcagggtct ggacgaacgc agccaggaca
    19561 tcatccgtgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    19621 gttacggtgt ttccgctgaa cgtgtgcgcc aactggaaaa gaacgcgatg aaaaaacttc
    19681 gcgccgctat tgaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    19741 gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac gtcaatcccc
    19801 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tggggtatgt
    19861 tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctga
    19921 atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg atgaatataa
    19981 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    20041 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    20101 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    20161 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    20221 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    20281 caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc accccagcgg
    20341 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    20401 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    20461 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg
    20521 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    20581 atttctctac gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg
    20641 gttaccaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    20701 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    20761 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca
    20821 ttgttgacgc gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    20881 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    20941 ccaaatacct gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga
    21001 aaggcgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    21061 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    21121 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    21181 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    21241 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    21301 gccgtaaacc ccagcgcctg cataaacgcc gtcatcacac cgcgatcttc caagcctgca
    21361 tccgccatcc accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat
    21421 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    21481 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    21541 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    21601 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    21661 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    21721 tcttttttat ttcttttggc aggtgattaa tt
//
LOCUS       DAZFKM010000080        21713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFKM010000080 DAZFKM010000000
VERSION     DAZFKM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..1385
                     /locus_tag="ACHKAY_003126"
     CDS             213..1385
                     /locus_tag="ACHKAY_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5701769.1"
                     /translation="MSTEPKETTASVRMSVAEKRSAEIRAKSLGYKSLSDYLRALASS
                     DMKSATPDPKKCRETRPVSLFHKTNFGAMWNGDSLDWMDSREAESVNLIMTSPPFGLV
                     RKKSYGNEDAHAYCDWFRPFAEGFKKILKDDGSLVIDIGGAWKAGTPTRSLYHYELLL
                     MLCNEYGFHLALEHFWWNPAKLPTPAEWVNVRRVRPKDAVNCVWWLSKTPFPKASNRR
                     VLAPYSDSMKSLLKNGYQAKTRPSGHVISEKFGKDNGGSVPPNLLAIANTESNGTYQD
                     YCRKKGLDIHPARFPSALPEYFIRMTTDPGDFVFDPFGGSCVTGAVSEALGRHWACVE
                     MNSAYLEGAMSRFEGDPIVLATDKPSSYTIFTPCAAQVVEEAVPLVADGGAARPKK"
     gene            complement(1387..2610)
                     /locus_tag="ACHKAY_003127"
     CDS             complement(1387..2610)
                     /locus_tag="ACHKAY_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701770.1"
                     /translation="MNSMSLDEICLRGLLRWVGTGSPSPYSGSVETLRYEDLDALRMW
                     WALSEPVGRLALRLVEKQREVSPTLDDFRREVSGELPGPPDAAASMLLQTVTLDPAAF
                     VVMEASSSWFSGPNRVLAKTLKTAQVALKAGALHARGGLFDLHANKRLALLDCALKVA
                     PIREVLSTPAGRSRIVAYEKRQASKARSPLYKQSWLCASILSDIEAFKADTIKALLAS
                     DLLPGMEVWRKFELATILEVAEALSGATGAPVVLDASFAAGRPAATIGDLELRWQRAM
                     PKRPSAFLDPGEVLAGELAFSLGVSKGTSRADITIERAGRILSIIECKWFSSPDSAPA
                     AILEATSQLVGYARDIAHAQEESDTGILARSIVALAHRGPAEMHFGAPVCCIGFSDFG
                     TANLAPWAAMVSGIH"
     gene            complement(2610..4298)
                     /locus_tag="ACHKAY_003128"
     CDS             complement(2610..4298)
                     /locus_tag="ACHKAY_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO5701771.1"
                     /translation="MATSLTTQDILGLFKALKDDIQATILKPRGLSIKSSGTKSLVLN
                     EPLNPETGFWQNPQAMSNKPLRWIPQVEVSAENVVARFAKNVDGQSIGMAKSVGYINN
                     RDGSCSVCVFRVLPAIKPLSPDSNWEFPAEWEFLLILGFAQPLPAGTSGDPLSNFEPS
                     EGTIDFGGGALKRTFPISFRALVKAPVSEEYVAGTSYSVVGNAIARLVGATVYDGTQA
                     SGRDALVTDLSNIFAPQSVEGPFSLDENTSLVGIEPSVYQQIEAAINSGKRHIILYGP
                     PGTGKTTLAEYLAAEVSERDDGDGAYLMLTASSAWSSQDLVGGYQPLGNGAIGFVPGA
                     LLRNFDKPVIIDELNRCPIDKVLGPLFSIFSGQSSTLPCRVEVANPASPFHVIYPKPH
                     PGMALHEHAPEPAWRMICTLNTYDKTQLGQISYALSRRFAWIKIGAPADPDGFVREMA
                     TRLGATVTEPVASNPIGALWKAVNSLREIGGAPIVDFIKTMLAFKSDVNLFVQPEGNT
                     ADLFLSAFRMCVLPLMDGLSPREARDLAESISVAWGLDAEKAASLTRDCAEFSA"
     gene            complement(4538..4840)
                     /locus_tag="ACHKAY_003129"
     CDS             complement(4538..4840)
                     /locus_tag="ACHKAY_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701772.1"
                     /translation="MKLLNIYEDKDEAEVALSKISGEKRLASERDGTVTIYNLFGQPT
                     WGNFYKLDLFNLRELQEILLLRKEGKPIDQKRYEEIIITLRYVSRSFDLSIPEHWL"
     gene            complement(4885..5331)
                     /locus_tag="ACHKAY_003130"
                     /pseudo
     CDS             complement(4885..5331)
                     /locus_tag="ACHKAY_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5437..6618)
                     /locus_tag="ACHKAY_003131"
     CDS             complement(5437..6618)
                     /locus_tag="ACHKAY_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5701773.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTLHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQTTDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHAQYLDGRREMMQWYADYIDSLSEQ
                     A"
     gene            complement(6795..6870)
                     /locus_tag="ACHKAY_003132"
     tRNA            complement(6795..6870)
                     /locus_tag="ACHKAY_003132"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6835..6837),aa:Phe,seq:gaa)
     gene            complement(6976..7683)
                     /gene="yqgA"
                     /locus_tag="ACHKAY_003133"
     CDS             complement(6976..7683)
                     /gene="yqgA"
                     /locus_tag="ACHKAY_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO5701774.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            7836..7919
                     /gene="yqgH"
                     /locus_tag="ACHKAY_003134"
     CDS             7836..7919
                     /gene="yqgH"
                     /locus_tag="ACHKAY_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO5701775.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            8081..10216
                     /gene="speC"
                     /locus_tag="ACHKAY_003135"
     CDS             8081..10216
                     /gene="speC"
                     /locus_tag="ACHKAY_003135"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO5701776.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(10266..11522)
                     /gene="nupG"
                     /locus_tag="ACHKAY_003136"
     CDS             complement(10266..11522)
                     /gene="nupG"
                     /locus_tag="ACHKAY_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO5701777.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(11724..12803)
                     /gene="mltC"
                     /locus_tag="ACHKAY_003137"
     CDS             complement(11724..12803)
                     /gene="mltC"
                     /locus_tag="ACHKAY_003137"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO5701778.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(12868..13143)
                     /gene="yggX"
                     /locus_tag="ACHKAY_003138"
     CDS             complement(12868..13143)
                     /gene="yggX"
                     /locus_tag="ACHKAY_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO5701779.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(13171..14223)
                     /gene="mutY"
                     /locus_tag="ACHKAY_003139"
     CDS             complement(13171..14223)
                     /gene="mutY"
                     /locus_tag="ACHKAY_003139"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO5701780.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            14384..15103
                     /gene="trmB"
                     /locus_tag="ACHKAY_003140"
     CDS             14384..15103
                     /gene="trmB"
                     /locus_tag="ACHKAY_003140"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO5701781.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            15103..15429
                     /gene="yggL"
                     /locus_tag="ACHKAY_003141"
     CDS             15103..15429
                     /gene="yggL"
                     /locus_tag="ACHKAY_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO5701782.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            15613..16332
                     /gene="yggN"
                     /locus_tag="ACHKAY_003142"
     CDS             15613..16332
                     /gene="yggN"
                     /locus_tag="ACHKAY_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO5701783.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            16508..17554
                     /gene="ansB"
                     /locus_tag="ACHKAY_003143"
     CDS             16508..17554
                     /gene="ansB"
                     /locus_tag="ACHKAY_003143"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO5701784.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            17671..18678
                     /gene="yggM"
                     /locus_tag="ACHKAY_003144"
     CDS             17671..18678
                     /gene="yggM"
                     /locus_tag="ACHKAY_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO5701785.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(18734..20035)
                     /locus_tag="ACHKAY_003145"
     CDS             complement(18734..20035)
                     /locus_tag="ACHKAY_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5701786.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(20035..20538)
                     /locus_tag="ACHKAY_003146"
     CDS             complement(20035..20538)
                     /locus_tag="ACHKAY_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5701787.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(20583..21569)
                     /locus_tag="ACHKAY_003147"
     CDS             complement(20583..21569)
                     /locus_tag="ACHKAY_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO5701788.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         5814 a         5184 c         5261 g         5454 t
ORIGIN      
        1 atacggcggt cggtttacgc ttacgaaaat tctccactgt catagtgaca attacaaaaa
       61 tcttcgtttc aaaacgaatt tggcaaagaa aggccttgcg attgatgtta agtggggaga
      121 aaatgacatc attaagtaaa tacgtttaac tgccttattt ttgtattaca aagaaaggaa
      181 attttaagtt ggagaagaac atggtacgcc caatgtcaac agaacctaag gagacgactg
      241 cctcagtgcg aatgagcgtt gctgaaaaaa gatcggcaga aatcagagct aaatccttag
      301 gctacaaatc gctaagtgac tatcttcgag cattggcgtc ctcagacatg aaatcggcaa
      361 ccccagatcc gaaaaaatgc agagaaacac gccctgtatc tctttttcac aaaacaaact
      421 tcggtgcaat gtggaatggt gactctctgg attggatgga ttcacgtgaa gcagaaagcg
      481 taaaccttat tatgacctcc ccacctttcg gtcttgtccg gaaaaaatcc tatggtaatg
      541 aggacgcaca cgcttattgt gattggtttc gcccatttgc tgagggtttc aaaaaaatac
      601 tcaaagacga tgggagctta gtgattgata tcggaggcgc atggaaggca ggaaccccca
      661 cacgctctct ataccattat gaactgctgc taatgctttg caatgagtac ggttttcatt
      721 tggcgctgga acatttttgg tggaatcctg ctaagttgcc gacacctgca gaatgggtaa
      781 atgtaaggcg ggtacgacct aaagatgccg taaattgtgt atggtggctt tcaaaaacac
      841 cattccctaa agcaagtaac aggcgcgtgc ttgcaccata ttccgactca atgaagtctt
      901 tactaaaaaa cggttatcaa gcaaaaactc gtccctcggg ccatgtgatt tctgaaaaat
      961 tcggcaaaga taatggtggt tccgttcccc caaatctcct tgctattgcg aatacagaat
     1021 caaatggcac ttatcaggat tattgtcgca aaaaaggatt ggacatccac ccagcgcgtt
     1081 ttccatcagc gcttccggag tattttattc gcatgactac ggatcctggc gattttgttt
     1141 tcgatccatt cggcggctct tgcgtaacag gtgcagtgtc tgaagccctt ggccgccact
     1201 gggcttgcgt agaaatgaat tcagcatacc ttgagggcgc aatgtcccgg tttgaaggtg
     1261 atcctatcgt tttagctaca gacaaaccat cctcttacac aatttttaca ccatgtgctg
     1321 cacaggtagt ggaagaggct gtccctttag tagctgatgg tggggccgct cgtccgaaaa
     1381 aataaattag tgtattcctg ataccatcgc tgcccaaggt gccaaattcg cggtaccgaa
     1441 atcagagaaa ccaatgcagc aaacaggggc tccaaaatgc atttcagcag gccccctgtg
     1501 agcaagagcc acaatgctac gagctagaat acctgtatca gattcttcct gcgcatgcgc
     1561 aatgtctctt gcataaccta caagctgact agttgcctca agtattgctg ctggtgccga
     1621 atcgggagaa gaaaaccatt tacattcaat aatcgaaaga atacgtccag ccctttcaat
     1681 agttatatct gctcgagaag tacccttgga aaccccaaga gagaaggcca gctcacctgc
     1741 tagcacctct cccggatcta aaaacgcact tggtcttttg ggcatggctc tttgccaacg
     1801 taattccaaa tcaccaatag ttgccgctgg cctgcccgcc gcaaacgaag cgtcaagaac
     1861 aacgggagcc cctgtcgccc ctgaaagggc ttcagcaact tccagaatcg ttgcgagttc
     1921 aaattttctc cagacctcca ttccaggaag taaatcggag gcaagtaggg ccttaatcgt
     1981 atcagcctta aaagcttcaa tatctgataa tattgatgca caaagccaag actgcttata
     2041 caacgggctc cttgccttgg aagcctgcct tttttcataa gcgacaatac gagatcttcc
     2101 cgcaggtgta ctaagtactt ctcgtatagg ggcgactttt agggcgcaat cgagaagagc
     2161 taatctttta ttcgcatgca aatcgaaaag acctcctctg gcatgtaaag ctcctgcttt
     2221 caaagctact tgcgctgttt ttagcgtttt cgcaagaacc ctgtttggcc ctgaaaacca
     2281 tgaagaagat gcctccatga ccacaaaagc tgcaggatca agagtgacgg tttgaagtaa
     2341 catagaagct gcagcgtcag gagggccagg aagttcacca gatacctccc ttctgaaatc
     2401 gtctaaggtg ggggaaactt ctctttgttt ttccactaat cttaaggcta gcctgccaac
     2461 aggttcagaa agagcccacc acattcttaa agcatctaaa tcttcatatc ttagagtctc
     2521 cacgcttccc gagtagggtg aaggagaccc tgttcctacc cagcgaagaa gaccgcgaag
     2581 acatatttca tcaaggctca tggagttcat cacgcagaaa actccgcaca atcacgagtt
     2641 aatgaagcgg ctttctcagc atctaggccc catgcaactg aaattgattc tgctagatcc
     2701 cttgcttctc ttggcgacaa accatccatc aatggaagaa cgcacatgcg aaacgcggat
     2761 aaaaacaagt ctgcagtatt accctctggt tgtacaaata ggtttacatc agatttgaac
     2821 gcgagcattg ttttgatgaa atcgacgata ggggcaccac ctatttcacg taatgaatta
     2881 acggccttcc acaaagcgcc aatcgggttt gaggctacag gctctgtaac tgtagcgcca
     2941 agacgagttg ccatttccct tacaaatcca tcaggatctg ctggcgctcc tattttaatc
     3001 catgcaaaac gacggctcag tgcataggaa atctgaccaa gctgggtttt atcataggta
     3061 ttcaaagtac agatcatacg ccatgcaggc tctggtgcat gctcgtgaag tgccattcct
     3121 ggatgaggtt taggataaat cacatgaaat ggacttgcag ggtttgccac ctcgacccga
     3181 caaggcagtg tcgaagactg tcctgagaaa atcgaaaaca acggtcccaa aactttatca
     3241 atcggacaac gatttaattc gtcaatgatt acaggtttat caaaattacg gagcaatgca
     3301 ccaggaacaa agccaatagc cccattacct agaggctgat acccgccgac aagatcttga
     3361 gaactccaag cagacgatgc tgtgagcatt agataagctc catcgccatc atccctctct
     3421 gaaacttccg ctgcaagata ttccgctaac gtcgttttac ctgtacctgg aggaccatat
     3481 aaaattatat gacgcttacc agagttgatt gctgcttcta tctgttgata taccgacggc
     3541 tcgatcccaa caagagaagt attttcatcg agtgaaaatg gtccttcaac tgactgagga
     3601 gcaaagatat ttgaaagatc agtaactaag gcatcacgtc ccgatgcttg agtcccatcg
     3661 tatactgtag caccaactaa ccgtgcgatt gcattgccaa cgactgaata tgatgttcct
     3721 gctacatact cttctgagac aggggctttc actagggccc ggaagcttat cggaaatgtt
     3781 ctctttaatg cgccgccacc gaaatcaata gttccttcac taggttcgaa attgcttaat
     3841 ggatcacctg acgtgcctgc gggcagaggc tgcgcaaagc caagaattag caggaactcc
     3901 cattccgcag gaaattccca atttgaatca gggcttagag gtttaatcgc agggagaacg
     3961 cgaaaaacgc atacagagca agagccatcg cggttgttaa tgtaaccgac gcttttagcc
     4021 atacctatgc tctgcccatc aacattttta gcgaatcttg caacaacatt ttcagcgcta
     4081 acttcaactt gaggaatcca gcgtaggggc ttatttgaca tcgcctgcgg attttgccaa
     4141 aagccagtct caggattcaa tggttcgtta agcacaaggc tctttgtacc cgagcttttt
     4201 atcgacagtc ccctcggctt caatattgtt gcctgaatat cgtctttcaa cgccttaaac
     4261 agcccaagta tgtcttgcgt cgtcagcgag gttgccataa tgttcccttc tttgtctgcg
     4321 tttttatctc cggatacaac aattttccat ctccttacct ttaggtcaac catataccgt
     4381 gaaactactt actcatgctc acttggtttg agaactcacg caaaaaaact ttctacaatg
     4441 caatttgcag gaaaaatctt tgttttgtca tcccaactga tctatgctga tgcatagatt
     4501 taacttaaag gcatagacag attgaattta gtggttttta taaccaatgc tcaggaatag
     4561 ataaatcaaa tgatctggat acgtatcgaa gagtaataat tatctcctca tatctctttt
     4621 gatcgatagg tttcccctcc tttcgcaaca gtaatatttc ttgcaattct cttagattaa
     4681 acaaatctag cttataaaaa tttccccacg ttggttggcc gaaaaggtta taaattgtga
     4741 ctgtgccatc cctctcacta gcgagtcttt tctctccaga aatttttgac aaagccactt
     4801 cggcttcatc tttatcttca taaatgttaa gcagcttcac tgttaacccc caacctcacc
     4861 tatataaaat agaaatcaaa accattactt ctgatagttc agtttaccaa aatcgaacgg
     4921 acttaccgct ttctcctgat tggcatccag aaaatctgtc caccactgta gcatcagttg
     4981 acgttcttct agatgttctg ctttatggat atacgcagca cgtacaccat tacgctcctg
     5041 atggctcatc tgaagttcca cagcatcgtg ggaccacaaa ccttattcaa tcaatgcact
     5101 gcgcgccatc gcccggaatc catggccaca gattacctgg gttgtgtcat attccataac
     5161 cctcagggct ttatttacgg tattttcact gattgggttg cgcgggtcat gtgctcccgt
     5221 aaagatcaga ccattttcac cagctcatgt cttaagttct gtcagtatgg ctacagcctg
     5281 tttgctgagt ggcacaaggt gcggtgttcg aatcctggtt ccccaatgag agttcaggcg
     5341 gtgcgagcaa ataaaaaaag aaaacataaa taaaaccatc cagctgttag ggctggatgg
     5401 ttattcatta ttttagtgtc actgagcttt cattcatcag gcctgctcag aaagtgaatc
     5461 aatgtagtca gcataccatt gcatcatttc acggcgaccg tcgagatact gggcatgatt
     5521 ataggtaccc ctgatggagt ttttatccac atgagctaac tgcatttcaa tccaggcaga
     5581 attaaagccc tgttcgtgca gaatggtact catggtgtga cgaaaaccat gaccggttgc
     5641 cctgccatcg taaccgatac gcttgagcac catgttgatg ctggcttcgc tcattggctt
     5701 gttgatatca caacgtcccg gaaaaaccag ccggtattgt ccggagaaag ttttgagctg
     5761 cagcagaatg tcagtggtct gtcgtgacag tggtacgaca tgtttacgtc gcattttcat
     5821 gcggctttca gggattgtcc acagggcatt atcaaaatca atttctgacc attcagcgga
     5881 ccgtagttca attgttctga ggcctgtcag aagcagcaga tttgtcgcca tgcgggtaac
     5941 aggactgcca tgatattcag cgagcttccg gagaaaaaca gggagctcat ctgctctcag
     6001 gtgggggaaa tgcgccgctt ttggcgccgc cagcgtactg gtcagttcag atgccggatt
     6061 gacagtggct cgtccggtgg cgatggcata acgaaaaatt tgattacagt attggcgcac
     6121 cttacgcagt ttttccagcg agccacgttt ttctatgcgg cgcagaattt ccagcatttc
     6181 cagcgggagg atatcagtaa cgcttcgttt gcccagatac gggaaaatat ctttatccag
     6241 agcctccagt acgcttctgg cgtaactttc agaccatgac aaagatttat gctggtgcca
     6301 ttccgtagca agggcatgga gcgtattggt ggcagagatt ttctgagtga gtttttcctt
     6361 tcgcttcttt tcgcccgggt caccaccttc ggagataatt tttcgggcat cgtcgcgttt
     6421 gattctggct tcagcaaggg agacgtcggg atagacgccg agggccagtt ttttctcttt
     6481 tcctgctatc cggtatttaa gccgccagta gcgggcaccc gtggttttta ccagcagata
     6541 taaaccacgt tcgtcagaga gtttatagtc tttttcttta ggtctggctg tttcgacctg
     6601 tctggctgta agtgccatat gggggctcct gaaactgact gaacacaatg agcccccacc
     6661 ggtgccccca atgaacgctt gattgagaga gagttcagta gagttcggtg gagttcagga
     6721 tgttcagatt gtttgtaacg atgtgattat acttgagttt cgtggagttc gggagatgaa
     6781 tctggaagtg aaagtggtgc ccggactcgg aatcgaacca aggacacggg gattttcaat
     6841 cccctgctct accgactgag ctatccgggc aacggggcgc attaaacctg attcgcctcg
     6901 tctcgtcaat caaatttctt caatttactg cagactgcac aatctatcat cactttgccg
     6961 ctattgcacg cattgtcagg caaaccaggc ggtccaggcg gcggaaagcg gcattgccag
     7021 aaggagtgct ggaagcatgt tgaccaccgg gaacatttta atgccacaga tgcgtaatcc
     7081 ggttgccagc agcaataaac cgcctacggc gctgaagtct gccatcatcg acggtgtggt
     7141 cagcggtaat atcagcgcgg cagcccacgc cagcgttaac tggatgatca gtaatgggat
     7201 actaattacc gacaccgcaa tacccagcga gcaggcgaag atcatcgccg taaagaaatc
     7261 aagaaatgac ttggcgatta aaatactcgg atcgccggtc atcccttcgt tcatcgcccc
     7321 gaagatcccg gtgccgctgg cgcaaaacag gacaataatc gcgacataat tctgaataaa
     7381 agattcatgc gctggcttct tacgtgagtg acgaaacaga ttttgtgctt tggcgactgc
     7441 tgtattgaca cctttctcca gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc
     7501 taaaaccatc gctggaaggt tggcgcattt cacaaccagt aaaataccaa tccccagcga
     7561 tgccagacca aaaatagatg tcatggagac tcggatacgt tccggtaagc gttggctgag
     7621 cagtgcgccg agaacgccac ccagtaagac tgcactagcg ttgataaaag ggccgatgac
     7681 cacaagagtt cctgttagct gtattgtttg attttcttat tatgaacttt ttgccttgtt
     7741 gatggcttgt ctggtcgcaa agtcctggct tgcacgcttt agcgaaaggt gccatgattg
     7801 cgcgaatttt ctcctctctg tacggagttt gcccgatgca cgccatctcc ttacattctc
     7861 tcgcttatcg ccgtttcgcg cgaaacgttt ccctttctat gttactgctc atgcggtgaa
     7921 gttaacgcac gctcactgca ggacaacagt aaaatcagag cgtttctgct tttactgatg
     7981 tctggcggtc ggagctggtg accagtttga cccatatctc atggggcagg gttttccacc
     8041 ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc
     8101 cgccagtagt gaactggtat cccgactttc ttctcatcgt cgcgtggtgg cgttgggaga
     8161 tactgatttt acggacgtcg cggcagtcgt cattaccgct gcggatagtc gcagtggcat
     8221 tcttgcgttg cttaagcgca ccggttttca tctaccggtg tttttgtatt ccgaacatgc
     8281 tgttgaatta cctgcgggcg ttacggcggt aatcaacggc aacgagcagc agtggctgga
     8341 gctggaatcc gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt atgacacgct
     8401 gacgcagtac gttgagatgg gcaacagcac ctttgcttgc cctggacatc aacatggtgc
     8461 gttttttaaa aagcatcctg ccggacgcca tttttacgat ttctttggtg agaacgtctt
     8521 tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc atgaaggatc
     8581 ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat gccgataaaa cctattttgt
     8641 gctgaacggc acatcggcag cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga
     8701 tctggtgctc ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc tgattcaggc
     8761 gggggcgacg ccggtctatc tggaagcttc acgcaacccg tttggtttca ttggcggtat
     8821 tgatgcgcac tgttttaatg aagagtatct gcgccagcaa attcgcgacg ttgcgccaga
     8881 aaaagccgag ctgccgcgcc cgtttcgcct ggcgattatt cagctgggaa cctatgacgg
     8941 aactgtctat aacgcccgtc aggtgatcga taccgttggg catctgtgtg attacattct
     9001 gtttgattcc gcgtgggtcg gttacgaaca atttatcccg atgatggcgg atagctcgcc
     9061 gctgctgtta gaacttaacg aaaacgatcc ggggattttt gtgacccagt cggtgcacaa
     9121 acagcaggct ggattctcac agacgtcgca gatccataaa aaagataacc atatccgcgg
     9181 acaggcgcgt ttttgcccgc ataagcggtt gaataacgcc tttatgctcc atgcttctac
     9241 cagccctttc tatccgctgt ttgctgcact ggatgttaac gccaaaattc atgaagggga
     9301 gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat
     9361 tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg
     9421 gcaggattat ccgacatctg tgttagccag cgaccgccgt tttttcagtt ttgagccggg
     9481 ggcgaagtgg cacggctttg aaggatatgc cgcggatcag tattttgttg atccgtgcaa
     9541 gctgttactc actacaccag gtatcaatgc tgaaactggc gaatatagcg actttggcgt
     9601 tccggcgacg attctggcgc actatctgcg tgagaacggc attgtgccgg agaagtgcga
     9661 tctcaactcc attctgttct tattaactcc ggcggaaagc cacgagaagc tggcacaact
     9721 ggtggcgatg ctggcgcaat ttgaacagca tattgaggat gactcgccgc tggctgaggt
     9781 gttgccgagc gtttataaca aatatccggt gcgctatcgc gactacaccc tgcgccagtt
     9841 gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat
     9901 gttccgccag cagagtttcc cgtcagtggt gatgaacccc caggatgcgc atagcgctta
     9961 tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga
    10021 aggggcgttg ccttatccac ctggcgtgct ttgcgtggta cccggggaag tctggggtgg
    10081 ggcggttcaa cgttatttcc ttgcactgga agaaggggtg aatttgttgc cgggattttc
    10141 gccggagctg caaggtgttt atagcgaaac cgatgcggat ggcatgaaac ggttgtacgg
    10201 ttatgtgttg aagtaagaat aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg
    10261 cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt atatttgaac
    10321 atcgccatga acgcgaaggc cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc
    10381 tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct cacgatgccg
    10441 ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc actggcgcga
    10501 attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa gaagtcgaat
    10561 gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg agtcgggtcg
    10621 ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat cattacgttc
    10681 ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt ttcagagatc
    10741 tgcgaaatcg acatgatgat tgacgcatgc tgcacaataa agctgctggc aaacatcgga
    10801 tctttgtcga agctgtgcag gaaggtatta ccgaacatat tggtaatctg cagttccgcg
    10861 cccagcagca ttgagaagat aaagaagatt gccatacgct tgtttttaaa cagcgcgaat
    10921 gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc aaccggaata
    10981 tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc aatatacagc
    11041 tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat gataaagccg
    11101 atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc ggcattttgc
    11161 aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc aaacgagtta
    11221 atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat gaacagcgtg
    11281 atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca tttgtcggcc
    11341 acaatcccca gcagcgcagg cataaagacc gcggcgatac ccagtgagct ataaactgcg
    11401 ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag ggtcgtcagc
    11461 caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag cttaagattc
    11521 atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc
    11581 gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg gcaaataatt
    11641 gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt gcccctgagc
    11701 atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc ggtattcact
    11761 ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt ctgataaaca
    11821 tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt cgaaaagact
    11881 cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg ccgcgatgtt
    11941 gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc ggtgccggta
    12001 tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc
    12061 gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc cagcgcatcg
    12121 gaacggctga ccgcatatgg gttaaaggaa gattcggtct gcataatcgc cagaatcagc
    12181 gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata tttgtgcgca
    12241 cgtttatcaa ggtggttcgg caccatgtta atggtaacgc tgtagatgat acgcagcccg
    12301 ttgctgcggc tcttcagacg gtttttcagc agataatccg cgaagttgct cgcgcgacct
    12361 tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa aggttctttc
    12421 gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc gtcacccatc
    12481 agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc ggcgatagtt
    12541 tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata ttgatcggtg
    12601 tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca gatgttctca
    12661 atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt gacccaggct
    12721 tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa
    12781 gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt ttatgcaagt
    12841 tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat agccctcgat
    12901 atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca gcttgcggtg
    12961 ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt tgtgctgcca
    13021 ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct cgccggggta
    13081 cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct
    13141 cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg
    13201 taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta agttatacca
    13261 gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc acataggcac
    13321 aatatctaag tggaaatggc tgaaggtatg ccgaaacgcg gtcagctgcg tcaggttatc
    13381 ggcagcaatc tgccgttgcg ccagccactg ccgcaaactt tcttcgtcgg caaactgcgg
    13441 gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca atacttcatc
    13501 ttcgtgctgt aatagcaaaa agtagccggt gcgctccggc agcgtctgtt tcggtttttt
    13561 gcccggataa agcgcccagc tattgttggc ggcggcaata catccgtttt gtagcggaca
    13621 gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca tcatcgcctg
    13681 attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc ataatttatt
    13741 ctcgacctct tttttccctg gccagccgct tacagcatag cagcgcgcca gcacccgttt
    13801 gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa tcgcacctgc
    13861 ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg ggaatttacc
    13921 gccgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc gggcgtaata
    13981 gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga gatcggtcac
    14041 cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa cctgagtttg
    14101 ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt gccagggcag
    14161 cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg
    14221 catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac ggcagggtgt
    14281 aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac taactttgga
    14341 taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa acgacgtcat
    14401 ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt ttgtgcgccg
    14461 ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc cggtgatggg
    14521 cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg aagcgccggt
    14581 gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta aagatcgccc
    14641 tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc
    14701 tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct
    14761 gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc ctgacccgtg
    14821 gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg aactggtaaa
    14881 aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac cttatgcgga
    14941 acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag agagcaatga
    15001 ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg gtcatcgtct
    15061 tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa gaaccgtagc
    15121 cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt ttcggtggca
    15181 tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga tgattttatt
    15241 aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct ggcctgggaa
    15301 ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt
    15361 aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt cgacgtttgg
    15421 tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta
    15481 tcaggcctag aaagatgcac gatcgagtag gcgggataag gtgtttacgc cgcatccggc
    15541 atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct gattttttct
    15601 tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt gacggcaatg
    15661 accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg
    15721 caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga cggcaacgtg
    15781 atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc gaaggattat
    15841 caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgaa
    15901 aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag cagcaaaatg
    15961 cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa
    16021 acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg cgccgaaggc
    16081 cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa tgaaatgggc
    16141 gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg aagcctgggg
    16201 gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt
    16261 ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc cctggtcggg
    16321 aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg tttaattctt
    16381 cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt tgtaaattgt
    16441 ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac gtaactggag
    16501 gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg gttttagtgg
    16561 tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca ttgccggtgg
    16621 tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag aaaatctggt
    16681 taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg tagtgaatat
    16741 cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga
    16801 ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg aagaaaccgc
    16861 ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg
    16921 cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg tagtggccgc
    16981 agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca ccgtactgga
    17041 cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt ctgttaacta
    17101 cggtcctctg ggatacattc acaacggtaa gattgactac caacgtaccc cggcacgtaa
    17161 gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga aagtcggcat
    17221 cgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag atgcgggcta
    17281 tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg tgttcgacac
    17341 cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg taccgacggg
    17401 tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg cctctggcac
    17461 gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa ccaaagatcc
    17521 gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta tcgtgccggg
    17581 gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg ataatcaccg
    17641 gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt tggcacggca
    17701 ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac tgaaaatatc
    17761 ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc tatcacctta
    17821 aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga tgtctcttcc
    17881 agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct cgaacagacc
    17941 tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg
    18001 tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg tgggcgggtg
    18061 gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga tttcaattat
    18121 cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc tgcgctggaa
    18181 aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc ggatgcatat
    18241 tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa aaaaattcac
    18301 caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt taaatatgac
    18361 aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga gtgttatgaa
    18421 gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca gacttttctg
    18481 caatcacaaa aactcagccg taaattgcag gatgactggg taacgcttga aaaagggcaa
    18541 tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat tttgatgaaa
    18601 attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca actgcggcgc
    18661 aatggggtga ttaagtaagt ttgatatagt gcctttgccg gataatataa aaagttatcc
    18721 ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat atttgcggaa
    18781 tgtaagtgat catcatcaac gctactagca atgcagcata aaatggcagc aatggtttga
    18841 ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca ctacccaccg
    18901 gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa tgtactgggt
    18961 ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt acagccggtg
    19021 tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc aatatgatca
    19081 gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata tcagcattag
    19141 tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc gaagtggtaa
    19201 caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg taaaccagca
    19261 ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg gtaaaaatgc
    19321 cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag gcatccagaa
    19381 acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca cggcgtttag
    19441 caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc aaatatcccg
    19501 ccataaatag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca attaacacgt
    19561 ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg gtagaaaatg
    19621 ccggatcata accttgtttg gtctgaatgg gattcagtgt gccaccaacg gcagcagctg
    19681 ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg ttcacatgac
    19741 caagtgaacc aggaacccga ccaaccatta cctgggcgag gttaatcaaa cgtatcgcaa
    19801 taccgccgct gttcatcagc gttccggcaa agatgaaaaa ggggattgcc aatagggcaa
    19861 agttatccag tccattagcc agtcgctgcg aaacagtgat tgccgcgata tcccagggaa
    19921 acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg ggcacaccaa
    19981 taaagaccaa cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta tccattaact
    20041 ggcctcctgc gcatgttgtt tcagttgttg aatgctttga ttaataaaac acaaggcata
    20101 aaacatcatc accaatccac ttaatggcag aatgatatat acgtatccca tcggtatttg
    20161 catcgcggca gatacctgtg atgttgccag cgttttatca attaatttca ggccaccagt
    20221 cacaatgacc gaaccggcaa aacctaaaat aagaacgtta atgactatac taagaagtaa
    20281 ttgcttgcgc ttattcaggg cgagagcaaa taaatcgata gagagatgtc tctgcgcgcc
    20341 gacggtatat gcagcaccta ataaccctac ccagatcatc aaaaatcgtg ccagctcatc
    20401 ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga ctacgcatac
    20461 aaccagagca agcatgacgg cgacagaaaa cgcggcaatg acgcggtcta ccgcaagttt
    20521 caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagagac tggcccggaa
    20581 tattattgag cagcgttgtc gaacttctct aacaaagcag cttgttttgg atcttttttg
    20641 aagtcgtcaa acagaggctg aactgcggca cggaatggtg ctttatccac ttttacaatt
    20701 tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc ccacaatttt
    20761 tgttgataag cttcggattt cttcgctgct gtttcgagga tttgttgctg ttctggggtt
    20821 aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc gtgttcatct
    20881 tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca cccaggaagg cacgttattt
    20941 tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa tgaaattgga
    21001 gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg ctgaacacga
    21061 attttcagcc ctttaagatc ttctggctta gtaatcggtt ttttcgcata gaaactgcgt
    21121 gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga gtccataatt
    21181 tctttaccaa cttcaccaaa aacaactttg ttgaagtgag cctgatcttt aaataagaac
    21241 ggcaaattat agatagcata aatattatca aaagactcca gatcactggc ggaacctttg
    21301 gtcatatcca gagcgccatt ttgcaacaac tccatagttt ctcgcgcatt acccatctgg
    21361 ctgctgggat aaatacggat caccatatta ccttttgaca gctgttttac ctctttcgcc
    21421 agctcttcaa aactctgatg tacaacatga cttctttcca ggttgtgtgc taactttaac
    21481 gtcacttttt ctgcggcgtt tacgccagtg ctaaccagag ccagcaagga tgcgcagact
    21541 aatgagcggg taaatgacct cattttcata aaaaaactct ctgttgaagg atggaaattg
    21601 gttgatatct aattgtgctt atcagtgctt tgtgcgacaa attggttaac ctgtctgaca
    21661 acttgtctat gtttatcaca acttgaaata aagtcagaaa ttaaatatta aat
//
LOCUS       DAZFKM010000081        21544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFKM010000081 DAZFKM010000000
VERSION     DAZFKM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            107..1753
                     /gene="mqo"
                     /locus_tag="ACHKAY_003148"
     CDS             107..1753
                     /gene="mqo"
                     /locus_tag="ACHKAY_003148"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO5701789.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(1902..2411)
                     /gene="eco"
                     /locus_tag="ACHKAY_003149"
     CDS             complement(1902..2411)
                     /gene="eco"
                     /locus_tag="ACHKAY_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO5701790.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2526..2690)
                     /gene="yojO"
                     /locus_tag="ACHKAY_003150"
     CDS             complement(2526..2690)
                     /gene="yojO"
                     /locus_tag="ACHKAY_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO5701791.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2799..3293
                     /gene="napF"
                     /locus_tag="ACHKAY_003151"
     CDS             2799..3293
                     /gene="napF"
                     /locus_tag="ACHKAY_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO5701792.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3283..3546
                     /gene="napD"
                     /locus_tag="ACHKAY_003152"
     CDS             3283..3546
                     /gene="napD"
                     /locus_tag="ACHKAY_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO5701793.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3543..6029
                     /gene="napA"
                     /locus_tag="ACHKAY_003153"
     CDS             3543..6029
                     /gene="napA"
                     /locus_tag="ACHKAY_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO5701794.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            6036..6731
                     /gene="napG"
                     /locus_tag="ACHKAY_003154"
     CDS             6036..6731
                     /gene="napG"
                     /locus_tag="ACHKAY_003154"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO5701795.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6718..7581
                     /gene="napH"
                     /locus_tag="ACHKAY_003155"
     CDS             6718..7581
                     /gene="napH"
                     /locus_tag="ACHKAY_003155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO5701796.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7578..8027
                     /gene="napB"
                     /locus_tag="ACHKAY_003156"
     CDS             7578..8027
                     /gene="napB"
                     /locus_tag="ACHKAY_003156"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO5701797.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            8037..8639
                     /gene="napC"
                     /locus_tag="ACHKAY_003157"
     CDS             8037..8639
                     /gene="napC"
                     /locus_tag="ACHKAY_003157"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO5701798.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8652..9275
                     /gene="ccmA"
                     /locus_tag="ACHKAY_003158"
     CDS             8652..9275
                     /gene="ccmA"
                     /locus_tag="ACHKAY_003158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO5701799.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9272..9934
                     /gene="ccmB"
                     /locus_tag="ACHKAY_003159"
     CDS             9272..9934
                     /gene="ccmB"
                     /locus_tag="ACHKAY_003159"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO5701800.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            9976..10713
                     /gene="ccmC"
                     /locus_tag="ACHKAY_003160"
     CDS             9976..10713
                     /gene="ccmC"
                     /locus_tag="ACHKAY_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO5701801.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10710..10919
                     /gene="ccmD"
                     /locus_tag="ACHKAY_003161"
     CDS             10710..10919
                     /gene="ccmD"
                     /locus_tag="ACHKAY_003161"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO5701802.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            10916..11395
                     /gene="ccmE"
                     /locus_tag="ACHKAY_003162"
     CDS             10916..11395
                     /gene="ccmE"
                     /locus_tag="ACHKAY_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO5701803.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11392..13335
                     /gene="ccmF"
                     /locus_tag="ACHKAY_003163"
     CDS             11392..13335
                     /gene="ccmF"
                     /locus_tag="ACHKAY_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO5701804.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13332..13889
                     /gene="dsbE"
                     /locus_tag="ACHKAY_003164"
     CDS             13332..13889
                     /gene="dsbE"
                     /locus_tag="ACHKAY_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO5701805.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13886..14938
                     /gene="ccmH"
                     /locus_tag="ACHKAY_003165"
     CDS             13886..14938
                     /gene="ccmH"
                     /locus_tag="ACHKAY_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO5701806.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(14973..15620)
                     /gene="narP"
                     /locus_tag="ACHKAY_003166"
     CDS             complement(14973..15620)
                     /gene="narP"
                     /locus_tag="ACHKAY_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO5701807.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            15940..18531
                     /gene="yejO"
                     /locus_tag="ACHKAY_003167"
                     /pseudo
     CDS             15940..18531
                     /gene="yejO"
                     /locus_tag="ACHKAY_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(18999..19922)
                     /locus_tag="ACHKAY_003168"
     CDS             complement(18999..19922)
                     /locus_tag="ACHKAY_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5701808.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            complement(19912..20073)
                     /locus_tag="ACHKAY_003169"
     CDS             complement(19912..20073)
                     /locus_tag="ACHKAY_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701809.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            complement(20085..20573)
                     /locus_tag="ACHKAY_003170"
     CDS             complement(20085..20573)
                     /locus_tag="ACHKAY_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5701810.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            complement(20701..20862)
                     /locus_tag="ACHKAY_003171"
     CDS             complement(20701..20862)
                     /locus_tag="ACHKAY_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO5701811.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            complement(20866..21060)
                     /locus_tag="ACHKAY_003172"
     CDS             complement(20866..21060)
                     /locus_tag="ACHKAY_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701812.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            complement(21191..21436)
                     /locus_tag="ACHKAY_003173"
     CDS             complement(21191..21436)
                     /locus_tag="ACHKAY_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701813.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
BASE COUNT         4845 a         5259 c         5982 g         5458 t
ORIGIN      
        1 gaatataatt tataaacatt atttaaatgt tgttacttaa gtgttaccgt tgatgccgcg
       61 caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt aacaagatga aaaaagtgac
      121 tgccatgctc ttctcgatgg ccgtggggct taatgccgtt tcgatggcgg caaaagcgaa
      181 agcgtccgag gagcaggaaa ctgatgtact gttgattggc ggcggcatta tgagcgccac
      241 gttggggacc tatttacgcg agctggagcc tgaatggtcg atgaccatgg tggagcgcct
      301 ggagggtgtc gcgcaggaga gttcgaacgg ctggaataac gccggaaccg ggcattctgc
      361 actgatggaa ctgaactaca ccccgcaaaa cgccgatggc agcatcagta ttgaaaaagc
      421 agtcgccatt aacgaagcat ttcagatttc ccgccagttc tgggcgcacc aggttgagcg
      481 cggcgtgctg cgtactccgc gttcatttat caataccgtt ccgcatatga gctttgtctg
      541 gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc gcgttgcaac aaagctcgct
      601 gtttcgcggt atgcgttact ctgaagatca cgcgcagatc aaagagtggg caccgttagt
      661 gatggaaggg cgcgatccac aacagaaagt agcggcaaca cgcacagaaa ttggcactga
      721 tgtgaactat ggcgagatca cccgccagtt aattgcttcc ttacagaaga aatctaactt
      781 ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt aatgacgata acacctggac
      841 cgttaccgtt gccgatctga aaaacggcac tgcacagaac atccgcgcca aatttgtctt
      901 tatcggcgcg ggcggtgcgg cgctgaagct attacaggaa tcggggattc cggaagcgaa
      961 agactacgcc ggtttcccgg tgggcggaca gttccttgtt tcggaaaacc cggacgtggt
     1021 taatcaccat ctggcgaagg tttacggtaa agcatccgtt ggcgcaccac cgatgtcggt
     1081 tccgcatatc gatacccgcg ttctggacgg taaacgcgta gtgctgtttg ggccatttgc
     1141 caccttctca accaaattcc tcaaaaacgg ttcattgtgg gatctgatga gttccaccac
     1201 cacctctaac gtgatgccga tgatgcacgt cgggctggat aatttcgatc tggtgaaata
     1261 tctggtgagt caggtgatgt tgagtgaaga ggatcgtttt gaagcgttga aagagtacta
     1321 tccgcaggca aaaaaagagg actggcgttt gtggcaagcg gggcagcgcg tgcagattat
     1381 caagcgtgat gccgagaaag gtggcgtact gcgtctgggt actgaagtgg tcagtgacca
     1441 gcaagggacc attgccgcgc tgctgggggc atcgccaggg gcgtcaaccg ccgcgccgat
     1501 tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt tccagcccgc aatggcaggc
     1561 tacgttgaaa gcgatcgttc cgtcttatgg acgcaagctg aacggtgatg tagcggcaac
     1621 agaacgcgag ttgcagtaca ccagcgaagt gctggggctg aaatacgaca agccgcaggc
     1681 agcagatagt acgccaaaac cgcagttgaa accgcaaccc gttcaaaaag aagtggcgga
     1741 tattgcgttg taatgatgtg ccacatccgg catggtatgc cggatgtggc gtatgctgat
     1801 aagacgcgcc agcgtcgcat caggcaccgg ctcgggcgtt agatgtcgga tgcggcgtga
     1861 ccgccttatt cggcctacgg ggtgccgcac ttcacagcat tttagcgaac taccgcgttg
     1921 tcaattttct cttccgcctt ccagacgcgg tacttcacat ctacattgtc tggcgtatac
     1981 accacgatcg gcagcttgct gttgtaacgc agcattccag catcgcccag atacgcggtg
     2041 acaaatttct tctctttctt gccatccggg caggccatca tcgtggaaac cggcgagcta
     2101 actttatcaa agacataata atcatagccc cagccttcca gcgttttgct ttccagcttc
     2161 ccgccgagac gatgcaaatt gcaatcgact tccagcgtct gaccgattaa cagttctact
     2221 ttcagggtag attcatcttc ttgcggggtt aactgaatca cctgacgctt catccctttt
     2281 tcagcttgtg gataaggcgc gattttttcc agtggctgga cgctttctgc cgcccaggcg
     2341 gaagtggtag cgaaagcggc aaacaatact gcaggtagaa tggtcttcat ttttttgctt
     2401 tccttcttca atttataggt tttcaccgat gatgtcgctg aaggttaaca tattcatcag
     2461 atttattaat ttgctttatg aataacttaa agaacattca gacaatttta cctgctcttt
     2521 tagtgttaaa ttctgatgag ggagcaaacc agcagaaata tctctggtga attaatcgct
     2581 atataaatat gtttataacc atttgaaatg tgagcaaaag cccgtttttg ccacgcttcc
     2641 cgccactctt ttgatcctgc tacaggtttt accccgatcg gggtatgcat ctttgacaca
     2701 tcctttaata tcttagcggc tataaaaatg gcttattaat tatgcggttt atttggtcgc
     2761 tctcaatttt cagagcgcgt taatgatgga aggtcaatgt gaagattgat gcatcccgtc
     2821 ggggcatact cactggtcgc tggcgcaaag ccagtaacgg tatccgtccg ccctggtccg
     2881 gtgatgaatc tcattttctg acccattgca cccgctgcga cgcctgtatt aatgcttgtg
     2941 aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag cgttaatttc aaaaataacg
     3001 agtgcagctt ctgttacgcc tgcgcgcagg cttgccccga atcgctattt tctccgcgcc
     3061 acaccagggc ctgggatttg cagttcacca ttggggacgc gtgcctggcg tatcagtcag
     3121 ttgaatgccg ccgctgtcag gatagctgtg agccaatggc aattatcttt cgcccgacgc
     3181 tctccgggat ctaccagccg caacttaata gccaactttg caacggatgt ggagcatgtg
     3241 cagccagctg tccggtatca gccataactg cggagtatct ccatgcacac taactggcaa
     3301 gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt cagacatcag cacccaactg
     3361 aacgcctttc ccggctgtga agttgctgtc agcgacgcgc cgagcggtca gttgattgtg
     3421 gtggtggaag cagaagacag cgaaacgctg atccaaacca ttgagtcagt acgcaacgta
     3481 gagggcgtgc tggcggtgtc gctggtttat caccagcagg aagagcaagg tgaggaaaca
     3541 ccatgaaact cagtcgtcgt agctttatga aagctaacgc cgttgcggcc gctgcggcgg
     3601 ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag gaagccatta
     3661 aatgggataa agcgccgtgc cgtttctgcg gtactggttg cggcgttctg gtcggaacgc
     3721 agcaggggcg tgtggtggcc tgtcagggcg acccggacgc accggttaac cgtggcctga
     3781 actgcattaa gggctatttc ctgcccaaga tcatgtacgg taaagaccgt ttgacgcagc
     3841 cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg cgaatttacc ccaatcacct
     3901 gggatcaggc cttcgatgtg atggaagaga aattcaaaac cgccctgaaa gaaaaaggac
     3961 cggaatcgat cggtatgttc ggttctggtc agtggactat ctgggaaggt tatgccgcgt
     4021 ccaagctgtt caaagcgggc ttccgttcga acaacatcga cccgaacgca cgtcactgta
     4081 tggcgtcggc agtagttggc tttatgcgta cctttggtat ggatgagccg atgggctgct
     4141 atgacgacat cgagcaggct gacgcgtttg tgctgtgggg cgctaacatg gcggagatgc
     4201 acccgatcct ctggtcacgc atcactaacc gtcgtctctc taaccagaac gtcaccgtgg
     4261 cggtgctttc tacctaccag catcgtagct tcgagctggc ggataacggc atcatcttta
     4321 cgccacagtc tgacctggtg atcctgaact acatcgccaa ctatatcatt caaaacaatg
     4381 cgataaatca ggacttcttc agcaagcacg ttaacctgcg caaaggggcg acggacatcg
     4441 gctacggttt acgtccgacc catccgctgg aaaaagcagc gaagaatccg ggttctgacg
     4501 cctccgaacc gatgagcttt gaagattaca aagccttcgt tgccgagtat acgctggaaa
     4561 aaactgccga aatgaccggc gtgccgaaag accagttaga acaactggcg cagctgtatg
     4621 ccgatccgaa caagaaagtc atctcctact ggacgatggg cttcaaccag catactcgtg
     4681 gcgtgtgggc caacaacctg gtctacaacc tgcacctgct gaccggcaaa atttcccagc
     4741 cgggttgcgg tccgttctcc ctgaccgggc agccttccgc gtgtggtact gcgcgtgaag
     4801 tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt gactaacgag aaacaccgtg
     4861 atatttgcga gaagaagtgg aatatcccga gcggcaccat tccggcgaaa atcggtctgc
     4921 acgcggtggc acaagaccgt gcactgaaag acggcaagct gaatgtttac tggaccatgt
     4981 gtaccaacaa catgcaggcc gggccgaaca ttaatgaaga gcgtatgccg ggctggcgcg
     5041 atccgcgcaa cttcatcatc gtctccgatc cgtatccgac agtcagtgcg ctggcagccg
     5101 acttgatcct gccgaccgca atgtgggtag agaaagaggg cgcttacggt aacgccgaac
     5161 gccgtactca gttctggcgt cagcaggtac aggcgccagg cgaagcgaaa tcggatctct
     5221 ggcagttagt ccagttctcc cgccgcttca aaactgaaga agtatggccg gaagagctgc
     5281 tggcgaagaa accggaactg cgtggcaaaa cgctgtacga agttctgtat gccacccccg
     5341 aagtgagcaa ataccctgta tccgaactgg cggaagatca gctgaacgat gaatcccgcg
     5401 agctgggctt ctatctgcaa aaagggctgt tcgaagagta cgcatggttt ggtcgcggtc
     5461 acggtcacga tctggcaccg ttcgatgact accacaaagc gcgcggtctg cgctggccgg
     5521 tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg taacgacccg tacgtgaaag
     5581 cgggcgaagg ctataagttc tacggtaaac cggatggcaa agcggtgatc ttcgcactgc
     5641 cgttcgaacc ggcggcggaa gcaccggatg aagagtacga cctgtggctc tctaccggac
     5701 gcgttctgga gcactggcac accggcagta tgactcgccg tgtgccggaa ctgcaccgcg
     5761 ccttcccgga agcggtcttg tttattcacc cgctggatgc gaaagcgcgc gatctgcgcc
     5821 gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt gatctcgatt gttgaaacgc
     5881 gcggtcgtaa ccgtccgcca cagggcctgg tgtacatgcc gttcttcgac gccgcacagc
     5941 tggttaacaa actgacgctg gatgcgaccg atccgctctc gaaagagacg gacttcaaga
     6001 agtgcgcagt caaactggag aaggtgtaag ccattatgtc ccggtcagcg aaacctcaaa
     6061 atggtcgccg ccgctttctg cgcgatgtcg ttcgcacagc aggcgggctg gctgccgtgg
     6121 gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc tggcgtgcgg ttgcgcccgc
     6181 ccggagccat aaacgagaac gcctttgcca gtgcctgtgt gcgttgcggt cagtgtgttc
     6241 aggcttgccc ttacgacacc ttaaaactgg cgacgctggc ctctggtctg tcggcgggca
     6301 cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg tgaggacatt ccgtgcgcca
     6361 aagtgtgccc aagcggtgcg ctggatcgtg agattgaatc gatcgacgac gcgcggatgg
     6421 ggctggcggt actggtggac caggaaaact gtctcaactt tcaggggctg cgctgcgatg
     6481 tttgttatcg cgaatgcccg aaaattgatg aggccatcac cctggagctg gagcgcaaca
     6541 cgcgtaccgg taagcacgcc cgctttctgc cgacggttca cagcgacgcc tgtaccggtt
     6601 gcggtaagtg cgaaaaagta tgcgtgctgg aacaaccggc aatcaaggtg ttaccgctgt
     6661 cactggcgaa aggggagtta ggtcaccatt accgcttcgg ttggctggag gggaacaatg
     6721 gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga aaaaaggctg gtggcgcagt
     6781 caccgttggc tggtgttacg tcgcctttgt cagttcttcg tgctggggat gtttttgagc
     6841 ggtccatggt ttggtgtgtg gatcttgcac ggcaactaca gcagtagcct gttattcgac
     6901 accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc ttgccagtgg tcatctgcct
     6961 gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc tctacgccct ggcgggaaag
     7021 cgattatttt gcagctgggt ctgcccgctg aacccgatta ccgacttagc gaactggtta
     7081 cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc gccacattcg ctacgtgctg
     7141 ttggtggtca ttctgctggg atcggcctta actggcacgc tcatctggga atggatcaac
     7201 cccgtatctc tgatggggcg cagcctggta atgggcttcg gcagcggcgc gctgcttatt
     7261 ctcgcgctgt ttttatttga tctactggtc gttgaacacg gctggtgcgg gtacatttgt
     7321 ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg tgattaccgt tgcggccacc
     7381 gatcggcaga aatgtaaccg ctgtatggat tgttttcatg tttgcccgga accgcatgtg
     7441 ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg tcaccagccg cgattgcatg
     7501 acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat ttacaataac tacacgatgg
     7561 agttcgggag cgaaatcatg aaaagccatg acctgaagaa agcgctgtgt caatggacgg
     7621 cgatgctggc cctggtggta agcggcgcgg tttgggcggc taatggcgtc gattttagcc
     7681 agtcgccgga agtatccggg acacaggaag gggccattcg gatgccaaaa gagcaggaac
     7741 ggatgccgct gaactatgtg aatcagccgc caatgatccc gcatagcgtt gaaggttatc
     7801 aggtaacgac caataccaac cgctgcttgc agtgccacgg tgtcgaaagt tatcgcacca
     7861 ctggcgcgcc gcgtatcagt cctactcact ttatggacag cgacggcaaa gtgggcgcgg
     7921 aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt accgcaggcc gataccgcgc
     7981 caatcgtggg gaataccttt accccatcaa aaggttacgg gaaataagag gttattatgg
     8041 gaaattctga ccgtaagcct ggtctgatta agcgcctgtg gaaatggtgg cgtaccccca
     8101 gccgtctggc gctggggacg ctgctgttga tcggttttgt tggcggcatc gtcttctggg
     8161 gtggctttaa caccgggatg gaaaaagcca ataccgaaga gttctgcatt agctgccacg
     8221 aaatgcgcaa cacggtgtat caggaataca tggattccgt gcactacaac aaccgtagcg
     8281 gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga gtttgtgccg aagatgatac
     8341 gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt tggcgttatt gacacgccgc
     8401 agaaatttga agctcatcgt ctgacgatgg cacagaatga gtggcggcgc atgaaggaca
     8461 ataactcgca ggagtgccgt aactgtcaca acttcgagta tatggataca accgcccaga
     8521 aatcggttgc cgcgaagatg catgaccagg cggtgaaaga tgggcaaacc tgtattgatt
     8581 gccataaagg gatagcgcac aagctgcccg atatgcgtga agtcgagcca ggtttttaac
     8641 agggttattg cgtgggtatg cttgaagcca gagagttact ttgtgagcgg gatgaacgaa
     8701 ccttatttag tggcttgtca tttacgctga acgcaggaga gtgggtacaa atcaccggta
     8761 gcaacggcgc ggggaagaca acgcttctcc gtttgctgac ggggttgtct cgccctgacg
     8821 caggcgaggt tctctggcaa gggcagccct tgcatcaggt acgcgacagc taccatcaaa
     8881 acctgttatg gataggccat cagccgggga tcaaaacccg gctgacggcg ttagaaaatc
     8941 tgcactttta tcatcgcgat ggcgataccg cacaatgtct ggaagccctg gcgcaggccg
     9001 ggcttgccgg attcgaagat attcctgtaa atcagctctc ggccgggcaa caacgccgcg
     9061 tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg gatcctcgac gagcctttta
     9121 ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg tatggcgcag catacggagc
     9181 agggggggat tgtgattctg actacccacc agccgctcaa cgttgctgaa agtaaaattc
     9241 gccgcatttc actgacgcaa acgagggccg catgatgttc tggcgcattt tccgtcttga
     9301 gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac ccgctgtggt tcttcctgat
     9361 tgtaattacc ctttttccgc tcagtatcgg tccggagccg caactgctgg cgcgtattgc
     9421 accgggcatt atctgggttg ctgcactgct ttcatccttg ctggcgctgg aacgactgtt
     9481 ccgtgacgat ttgcaggacg gcagtcttga acaattgatg ttgttgccgt tacccttgcc
     9541 cgccgttgtg ctggcgaagg tgatggcgca ctggatggta agcggtctgc cgttactcat
     9601 tctttcgcca ctggtagcaa tgctactggg aatggatgtt tatggctggc aagtgatggc
     9661 gctgacgctg ctgctgggaa cgcctacgct tggctttctc ggtgcaccgg gcgtggcgct
     9721 gacagtggga cttaagcgcg gtggtgtgct gctcagcata ctggtgttac cgctgactat
     9781 cccattactc atcttcgcca ccgccgcgat ggacgcggct tccatgcatt tgcccgttga
     9841 cgggtatctg gcaattttag gcgcgttgct ggcaggcacc gcgacattaa gtccttttgc
     9901 gacggcggca gcgttacgaa tcagcattca ataacgctgg attactgatt tttgttcgag
     9961 tctggtatcg aaactatgtg gaaaacactg catcaactgg cgatcccacc acggctgtat
    10021 caaatctgtg gctggtttat accgtggctg gcaattgcca gtgtggtcgt gcttaccgtc
    10081 ggctggatct ggggattcgg ctttgctccg gctgattatc agcagggaaa tagctaccgc
    10141 attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg gcatttatgc atcaatggca
    10201 gtggcagcgt ttattggcct tgtctggcag atgaaaatgg ccaacctggc ggtggcggcg
    10261 atggccccca ttggtgccgt gtttaccttt attgccctgg ttaccggctc tgcatgggga
    10321 aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc tgacttctga actggtgctg
    10381 ctttttttgt atgtgggtgt gattgccctg tggcacgcct tcgacgaccg ccgtctggcg
    10441 ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga atctgccgat tattcattac
    10501 tccgtggagt ggtggaacac cctgcatcag ggatcaacgc ggatgcagca aagtatcgat
    10561 ccggcgatgc gttcgccgct gcgctggtcg atttttggct tcctgctcct gtctgccacg
    10621 ctgacgctga tgcggatgcg taatttgatt ttgctgatgg aaaaacgccg tccgtgggtg
    10681 agtgaactga tactgaaaag aggccgtaaa tgacccctgc atttgcttcc tggaatgaat
    10741 ttttcgcaat gggcggttac gccttttttg tctggctggc ggtggtgatg accgttattc
    10801 cgctggtggt tttggtcgtg cactcggtga tgcagcatcg cgcaattctg cgtggtgtgg
    10861 cgcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca acagcaggag gctgcatgaa
    10921 tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtt ctggcagggt tggcactgac
    10981 tatcggtctg gtgctatatg cgctgcgctc gaatatcgat ctcttttata cgccggggga
    11041 aattctctac ggcaagcgtg aaactcagca aatgccggaa gtcggtcagc gtctgcgcgt
    11101 tggcgggatg gtgatgccgg gtagcgtgca gcgcgatccc aattcgctaa aagtaacttt
    11161 caccatttac gacgctgaag gttcagtaga tgtctcttac gaaggcattt tgccggatct
    11221 gttccgtgaa gggcagggcg ttgtggtgca gggcgaactg gaaaaaggca atcatatcct
    11281 cgcaaaagaa gtgctggcga aacacgacga aaactacacg ccgccagaag ttgagaaagc
    11341 gatggaagcc aatcaccgtc gcccggcgag tgtttataag gacccagcat catgatgccg
    11401 gaaattggta acgggctgct gtgtctggcg ctaggaattg cgctgctgct gtccgtgtat
    11461 ccgctatggg gcgtagcgcg cggagatgcg cgcatgatgg cgtcttcccg cttgtttgcc
    11521 tggctgctgt ttatgtctgt ggctggcgca tttctggtac tggtcaatgc cttcgtggtc
    11581 aacgacttca ccgtcaccta tgttgccagc aactccaata cccagcttcc ggtgtggtat
    11641 cgcgtggcgg ctacctgggg cgcgcatgaa ggctcgctcc tgctgtgggt gctgctgatg
    11701 agcggctgga cctttgcggt agcgattttt agtcagcgta ttccgctgga tattgtggcc
    11761 cgcgtactgg cgataatggg gatggtcagc gtcggctttt tgctgttcat tctctttacc
    11821 tctaacccgt tctcacgcac gttgccgaac ttcccgattg aagggcgcga tcttaacccg
    11881 ctgttacagg atccggggct gatcttccat ccgcctctgc tctatatggg gtacgtgggg
    11941 ttctcggtgg cgtttgcttt tgccattgct tctttgttgt gcgggcgtct ggacagcact
    12001 tatgcgcgtt ttactcgtcc gtggacgctg gcggcgtgga tcttcttgac gctcggcatt
    12061 gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg gcggctggtg gttctgggat
    12121 ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga ctgcgctgat gcactcactg
    12181 gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat tactgctggc aatcagtgcc
    12241 ttctcgttgt gtctgctggg gactttcctg gtgcgttccg gcgtgctggt atcggtacac
    12301 gcgtttgcct ctgatccggc acgcggtatg tttatcctcg cctttatggt actggtgatt
    12361 ggtggttcgc tgctgctgtt tgccgcgcgt ggacacaaag ttcgctcacg cgtaaacaat
    12421 gcgctgtggt cgcgggaatc tctgctgtta gcgaacaacg ttttgctggt cgccgcgatg
    12481 ctggtggtat tactggggac gctgctgccg ctggtgcaca agcaactggg actgggcagt
    12541 atttcgattg gcgaaccgtt cttcaacacc atgtttacct ggctgatggt gccgtttgcg
    12601 ctgctgcttg gtgtcggtcc tctggtgcgc tgggggcgcg atcgcccacg taaaatccgc
    12661 aatttattga ttatcgcctt catctcaacg ctggtgctgt cgctgctgct gccgtggctg
    12721 ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg caatggcctg ctggattgct
    12781 gtgctggcaa ttgcggaagc tgcgctgcgt atttcacgtg gcacgaaaac caccttcagt
    12841 tattggggaa tggtggcggc tcacctgggg ctggcagtga caattgttgg tattgccttt
    12901 agccagaact atagcgttga gcgtgatgtg cgcatgaagt ccggcgatag cgtcgatatt
    12961 catgaatatc gcttcacctt ccgtgatgtc aaagaggtga ctggcccgaa ctggcgtggc
    13021 ggtgtggcga ctatcggcgt aacgcgcgat ggcaagccgg aaacggtgct gtatgcggaa
    13081 aaacgttatt acaacactgc cgggtcgatg atgaccgaag cggcaattga cggcggcatc
    13141 acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa acggcgcgtg ggccgtgcgt
    13201 ctttactaca aaccatttgt tcgctggatt tgggcgggcg ggctgatgat ggcgttgggc
    13261 ggactactgt gtctgtttga tcctcgctat cgtaagcgcg tgagtccgca aaaaactgcg
    13321 ccggaggccg tatgaagcgc aaagtattgt taattccgtt gattatcttc ctggcgattg
    13381 ccgcggcgct gctgtggcag ctggcgcgta atgccgaagg ggatgatccg accaatctgg
    13441 aatcggcgct cattggcaag cctgtgccga agtttcgtct cgaatcactg gacaatccgg
    13501 ggcagtttta tcaggcggat gtgctgactc agggcaaacc agtactgctt aacgtctggg
    13561 cgacctggtg tccgacctgc cgtgcggaac atcaatatct gaatcagctt tcagcgcagg
    13621 gcattcgcgt ggtcggcatg aactataaag acgatcgcca gaaggcaatc agctggctga
    13681 aagagctggg caatccttac gcgctaagcc tgtttgatgg cgacggcatg ttagggctgg
    13741 atctcggtgt ctatggcgcg ccagaaacgt tccttattga cggcaacggc atcattcgct
    13801 atcgccatgc gggcgatctc aatcctcgcg tctgggaaga agagatcaag ccgctgtggg
    13861 agaaatacag taaggaggcc gcacaatgag gtttttattg ggcgtgctga tgctgatgat
    13921 ctccggctca gcgctggcga ccatcgacgt gttgcagttt aaagatgaag cacaggaaca
    13981 acagttccgt cagctcactg aagaactgcg ctgcccgaaa tgccagaata acagcattgc
    14041 cgattccaac tcgatgattg ccaccgacct gcgccagaaa gtgtatgaac tgatgcagga
    14101 aggtaaaagt aagaaagaga ttgtcgatta tatggtggcg cgttacggca acttcgtcac
    14161 ttacgatccg ccgttaacgc cgctgaccgt gctgctgtgg gtgctgccag tagtggctat
    14221 tggcattggc ggttgggtca tttacgcccg ctcgcggcgt cgggtacgcg tggtgccgga
    14281 agcgtttcct gaacaaagcg tgccggaagg taagcgtgcc ggatatgtcg cttatttgcc
    14341 gggcattgtg gtggcgttaa ttgtggctgg cgtcagctac taccagaccg gaaattatca
    14401 acaggtgaaa atctggcagc aggccacggc acaggctccg gcgttgctgg acagggcgct
    14461 ggatccgaaa gccgatccgc tcaacgaaga agagatgtcg cgtcttgcgc tggggatgcg
    14521 tactcaactg caaaaaaatc cgggagatat agaaggctgg attatgttgg gccgcgttgg
    14581 catggcgctg ggtaacgcta gtatcgccac cgatgcatac gctactgcgt atcgcctcga
    14641 tccgaaaaac agcgatgccg cactgggtta tgctgaagcg ttgacacgtt catctgatcc
    14701 caacgacaac cgcctcggcg gcgaactgct gcgccagttg gtgagaagcg accacagcaa
    14761 tatccgtgtg ttaagcatgt atgcgtttaa tgcctttgag cagcagcgat ttggcgaagc
    14821 cgttgccgcg tgggagatga tgttgaaact cttacctgcc aacgatactc gccgtgcggt
    14881 gattgaacgt agtatcgcgc aggcgatgca acatttgtcg ccgcaggaga ataaataaaa
    14941 agaggcccga tggtgatgcc atcgggctat ttttattgtg ccccgcgttg ttgcaggaac
    15001 agaatggtgg ctgccacgcg tgagcggaca ttgagtttgc gcagcagatt gcgaatatgt
    15061 acttttactg tctgctctga aatattcaac accgaggcaa tctgtttatt tgacagcccc
    15121 tgtgccagct cgtgcagaac atccagctcg cgctccgtca gcacgctgaa gggatcttct
    15181 tccgcgccaa acatttcacg ttcacgtaag tactgattga cgcgttcgct aaagactttg
    15241 ctgcctttcg ctccagcacg aattgcttcc agcaatactt ccgggtcgct gtctttcaac
    15301 agataaccgt ctgcgcctgc gtctatcagc gcaaagacat cgctggaggc atctgatacg
    15361 gtcaggataa taatttgcgc ggtaacgcca tccctgcgca aggcattgag ggtatccagg
    15421 ccactcatac ctttcatatt gagatccagc aagatcacgt cgatatccag tctattcgcc
    15481 agatcgatcg cgctcgcgcc gtcgcccgct tcggcgacca cttcaaagcc aggatcaagc
    15541 tccagtaact gacgaacacc gcgtcgcata agtggatgat catccacaat catcacctga
    15601 aaaggcgttg cttcaggcat agtagtctcc tgaggtttta ttagaatcat tatgtttgag
    15661 cgtagcgttg gtaacgctac caggcgcgtg aatgttaccc cattttatgt atggacatgt
    15721 agtgaaaact acaaccttgt cgttcgcttt ggcatcattt tcattataaa gacggaattt
    15781 tgcatcagct tctcatcaag aaaaacaact aaaaaatcaa tcacaatact tgtgcatttc
    15841 tttaaacagt ttttaaaaac tgactcccgg tatggagcta tgggtatttt ctgtacccaa
    15901 tgcttttaac agcaattaat ttcataggat gaaagctcaa tgcatcaatc tggttctgtt
    15961 tctctttgtc gttccgcaat atctgttctg gtggctacag cgttatattc acccatagca
    16021 ttggcatcaa cagttgagta tggtgagaca gttgatggtg ttgtcctgga aaaagatatc
    16081 cagctggttt atgggaccgc caataatacg aaaatcaatc ctggcggaga acagcatata
    16141 aaagaatttg gtgtaagtaa taatactgaa attaacggag ggtatcagta cattgaaatg
    16201 aatggcgccg cagaatactc agtattaaat gacggttatc aaattgttca aatgggtggc
    16261 gcggcaaacc agactacgct caataatggt gtgctacagg tttatggcgc agcgaatgat
    16321 accacgatta aaggcgggcg cttaatcgtt gaaaaagatg ggggggccgt ctttgtcgct
    16381 atcgaaaagg gaggactact ggaggttaaa gaggggggat tagcgattgc ggtagatcag
    16441 aaagcaggcg gtgctattaa aacaaccacg cgggccatgg aggtattcgg aacaaaccgt
    16501 ctcggtcagt tcgatatcaa gaatggtatt gctaataata tgttgttgga aaacggcgga
    16561 agtttgcgag ttgaagaaaa tgacttcgct tataatacca ctgtagatag tggcggctta
    16621 ctggaggtta tggatggcgg gactgtaact ggcgttgata aaaaagcagg cggaaaatta
    16681 attgtctcaa cgaatgcgct ggaagtgagt ggtccaaaca gtaaaggcca atttagtata
    16741 aaagatggtg tgtcaaaaaa ttatgaactg gatgatggtt ccgggctcat tgttatggag
    16801 gacacgcagg ccattgatac tatccttgat gagcatgcca ctatgcaatc gctgggaaag
    16861 gatactggta cgaaagtgca ggcaaatgcg gtatatgatc tcggtcgatc atatcagaat
    16921 ggaagtatca cgtattcctc aaaagccatc tctgaaaata tggttatcaa caatggccgc
    16981 gctaacgtct gggctggcac aatggttaac gtttcagtca gagggaatga tggcattctt
    17041 gaggtcatga agccgcaaat aaattatgca cccgcaatgt tggtgggtaa ggtagtggtt
    17101 tctgagggcg cttcttttag aacgcatagt gccgtggata ccagcaaagc ggacgtttcg
    17161 ctcgaaaata gcgtatggac catcattgcc gatatcacta cgacgaacca aaacaccctc
    17221 ctcaacttag ccaaccttgc gatgtctgac gcaaatgtga ttatgatgga tgagccagtg
    17281 actcgttcat cagtgacggc aagtgcggaa aatttcatta cgttgaccac caataccctg
    17341 tcgggaaacg gcaattttta tatgcgtacc gatatggcta atcatcagag cgatcagctc
    17401 aacgtcaccg gtcaggcaac aggtgatttc aaaatattcg tgacggacac cggtgccagc
    17461 ccggcagcag gagatagcct tacactggta acaacgggcg gcggtgatgc tgcatttacg
    17521 ttgggcaatg ccggaggcgt tgttgatatc ggtacgtatg aatatacctt gctggataat
    17581 ggcaaccata gctggagttt ggcagagaat cgcgcgcaaa ttaccccttc aaccactgat
    17641 gtgctgaata tggcggcagc acaaccgctg gtatttgatg cagaactgga caccgtgcgt
    17701 gagcgtcttg gtagcgtaaa aggcgttaat tacgatacgg cgatgtggag ttcggcaatt
    17761 aacacccgca acaacgtgac cactgatgcg ggcgctggct ttgagcaaac attgaccggg
    17821 ctgacgctcg gtatcgatag ccgtttctcc cgtgaagaaa gcagcacaat tcgcggcttg
    17881 ttctttggtt actctcattc tgatattggt tttgatcgcg gcggcaaagg caatgtcgat
    17941 agctataccc tgggggctta tgccggttgg gagcatcaga acggtgccta tgttgatgga
    18001 gtggtgaaag ttgaccgttt tgccaacacc atccatggca agatgagtaa tggggcaaca
    18061 gcgtttggcg attacaatag taacggcgcg ggtgctcatg tcgagagcgg gttccgttgg
    18121 gttgacggat tgtggagtgt tagaccctat ctggctttta ccggctttac cacagatggt
    18181 caggactaca cgttatcaaa cggcatgcgc gcggatgtgg gaaatacccg gatattacgc
    18241 gctgaagcgg gaacggcggt aagctatcac atggacctgc aaaacggtac gacgctggaa
    18301 ccctgactga aagccgccgt gcgtcaggaa tacgccgatt ctaaccaggt gaaagttaat
    18361 gacgatggca aatttaataa tgatgtggct ggaacccgtg gcgtttatca ggctgggata
    18421 aggtcatcgt ttaccccgac gttaagcggt catttgtcag tcagctatgg caatggcgca
    18481 ggggtagaat cgccgtggaa tactcaggcg ggtgtggtct ggacgttctg ataacaaaaa
    18541 ataaacaggc tgtgatgtgt cacggtctgt ttatcgaatt aattgcagat ataaaaaaaa
    18601 ccaaccgtaa aggttggttt tttcttggga tttttggcac gagagtagtt taattgagta
    18661 tgtaactaca tgattttaaa tagtatgctt gattcaactt ttaaaccgta taactaaacg
    18721 tataactaaa atcatttgct gaaaaagtgt acaatttaaa tgcgagtttt cctgtggtaa
    18781 aagctccaac cgtaacgatg ttcgttatgg ttgccttgat ggtctgcgtg gtcattatga
    18841 ccattcagga tttgaagggt gacgcttccg acttcctcaa attgaggttt ccgaaaatat
    18901 cagcgggtta gcgattaatc tgaccattaa ttaaactgcg aaaatttcgt aattatcccg
    18961 atatggggat caccatatcg ggattgatgc cggaaaaatc agcttttcgc cagaacggtg
    19021 acggccttat cgagtttttg ccatccgcaa taggtgattg ccctgaacgc tgtagcgtca
    19081 ttctggaaaa ggctaaccgt ccctgattcg gtttttatta ccgcctcatc agttggggtt
    19141 ataaccgtgg cttcaacgtt aataatcagc ttttgagcgt ctatcagcat catgcgatcc
    19201 gcctgttcgc tcacaattac cggtattctc aaaagctcta tctgccagaa ctcagcatca
    19261 gtaagctgta aggcgttttc tacgggcaag attagcgacg acgtggcaag cgtgttaccg
    19321 ttcgctatgt gctttttgaa aagctctatg aagttttcgg cctcatcagc accattaagc
    19381 acgccctcag gggatacgcc cgccacctcc tggtcactgg aaagaaattt tttatctatt
    19441 tctttcaccg attccgatgt gatcaagtct ctgatcgctg aattggtacc aggggtgcta
    19501 aatttgatta gctcgttatt cagcatcaca atagcgccaa gtttgcacag ttttagcgca
    19561 ctttcgttta cttctccctc tttgacaggg atttcttcgg cttctttaag ccatttacta
    19621 cccttgcctg atattgactg caattttgcg ccaaacggaa gattcagcgc gctgctgatt
    19681 agcttgcctg gtacagactg cgaaaaaata tactcgccaa gctctcttac tatcggctct
    19741 gccaccagcg acggcatatt atccgtagtc attccggcaa caatggattt ttcaagaaat
    19801 ttcgatactg ccggatcatt ttttttcgtc atttcaattg cgtctgcatg gctgaattta
    19861 cacgatgtaa gcacgctggc ggcgcgaact gccgacacgc ctttcatgtt tttagaaatc
    19921 attatcttct tcctcctggt aacatggggt tagggtttcg tgaaaatatg gcatcagaac
    19981 agcggcagcc tgtaaccgta tttcggttgg ctgctgcttg tcgttcatga tttccgtgag
    20041 tacatcaata gggtctgtga tgtattcgct catagttcgc tacctcagtg ttttaagcca
    20101 gttggtggct tagggatggg gaaccgattt gaggagacat tagcggcatt gagtttttta
    20161 acctcttttt tcccaatgct gccgatcttg cggtgggtga actgggccag cttaaacgcg
    20221 gcatctaaag ccagttttgg atctgtgtcc atgttgtcaa tcagtatcct gcccattgct
    20281 ttgatggggt cgggtaggcc gttatccatc atctcaatgg tgttaattga tgcccgttcc
    20341 ggtgtaactt tttcggtaac tttctttgta acttttttcg gttttgtaac ctgaaaagtt
    20401 acatcagaag ttacagcctt aagatacttt ttcacctccg gatcattgga tagctgccag
    20461 cctttaaccc ttgcagtctt ttcgctgtat cccgcatgta tcgcggcttc cgttggattt
    20521 gtgccctttg ataacgcatc agcaaaccgc ttttttttgg tcgttaatgc catgcaaaaa
    20581 cctcgttttg agggggtgta acttttcgtg taactttttt gaaaagcaaa attttctatg
    20641 aatgagactg ggggcggtgt ccagggcgat cgactttccg gccggaacac tccccccctg
    20701 tcattgctca ccgagtaacg agatcaggcc ttcaacttca tcactcagct tgtttattcg
    20761 tgtctcaagc tctgcgatct tttccatcaa tggcgctatg gtgtctcttg tttcctcgcc
    20821 aatggcttta atgatgcttg cctcgcgttc tgtgatagcc attgtttacg cctcacttgt
    20881 tgcaatgccc aggcgttcgc gtaacagtac gcgcgcaaga tgccctattg ttggtgttgc
    20941 cgccctgccg tagatacctg attcattcat ccggcgcaca atatcgaact gcaacttttt
    21001 aagctcgtta aggatttcat gatcaatcat cacggtagcg ctttgcttta tctcttccac
    21061 tttgttttct cctgatactt atcattgcat aaccagtatt ttcgatagat tatcattaat
    21121 taaccatctt tgaagatata tgagatcgaa aaaaagccgg attgctccgg ctatgtgctc
    21181 acttcttcgg ttattctcca gcgcttttaa cgttgatggt tgttacctgt tccgcttcgg
    21241 caatctcccg ttctgtcagc gtggcaaagt ttgccgccgc tgtggtcatg aatgcgctta
    21301 tcagttcggg atgctccttc gcgtatcctt cccccgcgtg gcggtctatc gttctgattg
    21361 ccacctttaa ggaaagctct gtcatgtcta acgctttata ttttggcgct gtcttatctc
    21421 tggtttttct gttcattccc cactactccc cacttttgct ccccactttt tgaaattacc
    21481 caccgtctcc ccacctcttt tttttgtgat tctaacgtta tgtttttcaa tatgtttttt
    21541 actc
//
LOCUS       DAZFKM010000082        21133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFKM010000082 DAZFKM010000000
VERSION     DAZFKM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..259)
                     /locus_tag="ACHKAY_003174"
     CDS             complement(<1..259)
                     /locus_tag="ACHKAY_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO5701814.1"
                     /translation="MRDNGLNNMFFSLCQINDNHSFTSSSHTKKTKSYNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDR"
     gene            complement(437..2086)
                     /gene="actP"
                     /locus_tag="ACHKAY_003175"
     CDS             complement(437..2086)
                     /gene="actP"
                     /locus_tag="ACHKAY_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO5701815.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2083..2397)
                     /gene="yjcH"
                     /locus_tag="ACHKAY_003176"
     CDS             complement(2083..2397)
                     /gene="yjcH"
                     /locus_tag="ACHKAY_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO5701816.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2598..4556)
                     /gene="acs"
                     /locus_tag="ACHKAY_003177"
     CDS             complement(2598..4556)
                     /gene="acs"
                     /locus_tag="ACHKAY_003177"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO5701817.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4948..6384
                     /gene="nrfA"
                     /locus_tag="ACHKAY_003178"
     CDS             4948..6384
                     /gene="nrfA"
                     /locus_tag="ACHKAY_003178"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO5701818.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6429..6995
                     /gene="nrfB"
                     /locus_tag="ACHKAY_003179"
     CDS             6429..6995
                     /gene="nrfB"
                     /locus_tag="ACHKAY_003179"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO5701819.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6992..7663
                     /gene="nrfC"
                     /locus_tag="ACHKAY_003180"
     CDS             6992..7663
                     /gene="nrfC"
                     /locus_tag="ACHKAY_003180"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO5701820.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7660..8616
                     /gene="nrfD"
                     /locus_tag="ACHKAY_003181"
     CDS             7660..8616
                     /gene="nrfD"
                     /locus_tag="ACHKAY_003181"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO5701821.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8636..10354
                     /gene="nrfE"
                     /locus_tag="ACHKAY_003182"
     CDS             8636..10354
                     /gene="nrfE"
                     /locus_tag="ACHKAY_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO5701822.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10347..10730
                     /gene="nrfF"
                     /locus_tag="ACHKAY_003183"
     CDS             10347..10730
                     /gene="nrfF"
                     /locus_tag="ACHKAY_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO5701823.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLRLMALILWRVRAKR"
     gene            10727..11323
                     /gene="nrfG"
                     /locus_tag="ACHKAY_003184"
     CDS             10727..11323
                     /gene="nrfG"
                     /locus_tag="ACHKAY_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO5701824.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11665..12978
                     /gene="gltP"
                     /locus_tag="ACHKAY_003185"
     CDS             11665..12978
                     /gene="gltP"
                     /locus_tag="ACHKAY_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO5701825.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(13056..13745)
                     /gene="yjcO"
                     /locus_tag="ACHKAY_003186"
     CDS             complement(13056..13745)
                     /gene="yjcO"
                     /locus_tag="ACHKAY_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO5701826.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13839..15986)
                     /gene="fdhF"
                     /locus_tag="ACHKAY_003187"
     CDS             complement(13839..15986)
                     /gene="fdhF"
                     /locus_tag="ACHKAY_003187"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15567..15569),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO5701827.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16184..17650)
                     /gene="mdtP"
                     /locus_tag="ACHKAY_003188"
     CDS             complement(16184..17650)
                     /gene="mdtP"
                     /locus_tag="ACHKAY_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO5701828.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17647..19698)
                     /gene="mdtO"
                     /locus_tag="ACHKAY_003189"
     CDS             complement(17647..19698)
                     /gene="mdtO"
                     /locus_tag="ACHKAY_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO5701829.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19698..20729)
                     /gene="mdtN"
                     /locus_tag="ACHKAY_003190"
     CDS             complement(19698..20729)
                     /gene="mdtN"
                     /locus_tag="ACHKAY_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO5701830.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20748..21023)
                     /gene="ytcA"
                     /locus_tag="ACHKAY_003191"
     CDS             complement(20748..21023)
                     /gene="ytcA"
                     /locus_tag="ACHKAY_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO5701831.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4788 a         5566 c         5753 g         5026 t
ORIGIN      
        1 tcctgtccgt ttccttcgtg atttttcctg atggtaaact atcataaatt cttttcattt
       61 tctgaatcaa acctatcact ttttcatgat catccatctt gaaaagagag agggctttat
      121 tgtcaattaa cgtgttttta tgatgtttgc tgtaattata tgattttgtt ttctttgtat
      181 gtgatgaact tgtgaaagag tggttatcgt taatctggca aagagagaaa aacatattat
      241 ttaaaccatt atcgcgcata aaatatcctt ataattaaca agaaagaaaa ggcatattct
      301 ctgcattatc attttctacg actgtcaaaa atcgctcatt ttttaatgag tttatttgtt
      361 taatattatg ggaaaaggtg atgcatgctg gagaggaaga gtattccccg gtcaggcgac
      421 cggggaaggg gagagattaa tgtgcgcggc cttgctcaac gccaaagccg gtctgggagc
      481 ggataaactg tgcacggaac agttcacgct cgcgcgcacc ttccgctgag ttatcggttg
      541 ccgagaagaa ccagatgccg aggaatgcca cgctgataga gaacagcgcc gggtattcat
      601 acgggaagat ggctttttcg tgaccaagga tctgtaccca aatcgtcggg ccgaggatca
      661 tcagtaccac tgcggtaatc agccccagcc agccacccat catcgcgcca cgagtggtca
      721 gtttcgacca gtacatagaa agcagaatga tcgggaagtt acagctcgct gcgatggcaa
      781 acgccaggcc taccatgaag gcgatgttct gattctcaaa cagcacgccg aggataatgg
      841 caatcacacc taaaatcagc acggtgattt tcgatacccg cagttcttca cgttcggtcg
      901 cgcctttttt gaagacgtta gcgtacaggt catgcgaaac cgctgatgcg cccgccagcg
      961 tcagaccagc aaccaccgca agaatggtgg caaaagcaac cgctgaaata aaaccgagga
     1021 acaggttgcc gcccactgca ttcgccaggt gaacggccgc catgttgtta ccaccaatca
     1081 gatggcccgc cgcgtcttta tattccggat tcgcaccaac cagcatgatc gcgccgaagc
     1141 cgataataaa ggtcagaata tagaagtagc ccataaaccc ggtggcgtag aacacgctct
     1201 tacgtgcttc gcgggcatcg ctgactgtaa agaagcgcat cagaatgtgc ggcaagcccg
     1261 ccgtaccaaa catcagtccc agacccagag agagcgcgga gatcggatct ttcaccagcc
     1321 cgcccggctt catgatgtcg acacctttcg ggtgtaccgc catcgcttca ctgaacagat
     1381 tgttgaagct aaagccgacg tgtttcatca ccataaaggc cataaagctg gcaccgaaca
     1441 gcaacagcac ggctttgata atctgtaccc aagtggtcgc cagcatgccg ccgaacagga
     1501 cgtacatcat catcagcacg ccgaccagca ccaccgcaat gtgatagtta aggccaaaca
     1561 gcagctcgat cagtttacct gcgcccacca tctgggcgat aaggtaaagc gccaccacca
     1621 ccagagaacc acaggccgaa agaatacgaa tcggcccttg tttcagacgg taagaggcca
     1681 catcggcaaa ggtgtagcgc cccaggttac gcagacgttc ggcaatcagg aacaaaatga
     1741 tcggccagcc caccaggaag cccagcgagt aaattaagcc gtcatagccg gaggtaaaca
     1801 ccagcgcgga gatccccaag aatgaggcgg cggacatata gtccccggca atcgccagcc
     1861 cgttctggaa gccagtgata ttgccgcctg cggtgtagta gtcgctacga gaacgtacgc
     1921 gttttgatgc ccagtaggta atgccgagcg taaacacgac gaaaatcagg aacataataa
     1981 tcgcctgcca gttcgttggc tggcgctcta cggccccgct aatagcatcc gcggcgttag
     2041 ctgcgaaagg gagtgtggcg gcaagcgccg tcagaactct tttcatgatg cttgtacctc
     2101 atgcaggact tcattattaa gacggtcgaa ttcgccgttc gcccgccaga tgtagatacc
     2161 ggtgagaaca aaggagatca caatcactcc aacaccaatt ggaataccgc gtgtgacgct
     2221 ggtgttcgga ttcagcggcg tgcccagcca gccgggcgcg aaggcgatca gtaaaataaa
     2281 gccgatataa actgccagca taataatcga caggatggtg gcaaaccgtt gccgtttttc
     2341 gactaactcc ctgaaatgcg cattgtcttc tatccgctga taaatcgtac cattcatcac
     2401 agattctcca gaggtaatgt agggattgtt ttaattcccg ctcccttatg ggagaaggtt
     2461 aacgctcggg taacccttgc cgaatgtagg ccggataagg cgttatacgc cgcatccggc
     2521 aatcaatgcc tgatgcgacg ctgtcgcgtc ttatcaggcc tacaaaccgt taccgactcg
     2581 catccggcaa ttgtgggtta cgatggcatc gcgatagcct gcttctcttc aagcagcttc
     2641 tcgactacgc caggatcggc aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc
     2701 gcaattttgc gcagaatacg gcgcataatt ttgccggagc gggttttagg cagggagtcg
     2761 gtccagtgca gcacgtctgg cgtcgccagc gggccaatct ctttacgcac ccagttgcgg
     2821 acttctgcgt acagttctgg tgacggttcc tccccgtgat taagcgtgac gtaggcgtag
     2881 atcgcctgac ctttaatatt gtgcggaata cctactacgg cggcttcggc aatcttcgga
     2941 tgcgccacca gcgccgactc aatctctgcc gtccccagac ggtgaccgga gacgttcagc
     3001 acgtcgtcca cacgcccggt gatccagtaa tagccatctt catcgcgacg cgcgccgtcg
     3061 ccgctgaaat acatattttt gaaggtggag aagtaggtct gttcaaaacg ttcgtgatcg
     3121 ccaaacagcg tacgcgcctg acccggccag gagtcggtga tcaccaggct gccttcggta
     3181 gcgccttcca gcgggttacc ttcgttatcg accagcgccg gttgcacgcc gaagaacgga
     3241 cgtgttgccg aaccggcttt cagctcggta gcgccaggca gcggcgtgat catgaaaccg
     3301 ccagtttcgg tctgccacca ggtatcgacc accggacatt tctcgttgcc gatttttttc
     3361 cagtaccact cccacgcttc cgggttaatt ggctcgccca cggaaccgag aatgcgcagc
     3421 gacgaacggt cggtaccctc gatcgcttta tcgccttccg ccatcagcgc gcggatcgcc
     3481 gtgggtgcgg tatagagaat attgacctga tgcttgtcca ccacctgcgc catacgggca
     3541 ggcgtcggcc agttgggtac gccttcaaac atcagcgtgg tcgcaccgca ggccagcggg
     3601 ccgtacagca agtaactgtg tccggtcacc cagcccacat cggcggtgca ccagtagata
     3661 tcacccggat gataatcaaa gacatattta aaggtcagcg ccgcgtacac cagataaccg
     3721 ccggtagtat gcagcacacc ttttggctta ccggtagaac cggaggtgta gagaataaac
     3781 agcggatctt cggcgttcat ctcttccgcc tggtgctgat cgctcgcttg ctcaaccagg
     3841 tcgtgccacc acaggtcgcg cccttcctgc cagtcaattt tcccgccagt acgcttcagt
     3901 accaccacat gctctacgct ggtgacgttc gggtttttca gcgcgtcatc aacgtttttc
     3961 ttcagcggaa tactgcgccc ggcacgcaca ccttcgtcgg aagtgatcac cagtcgtgag
     4021 ttggaatcaa taatgcgccc ggcaacggct tccggcgaga agccgccgaa aatcaccgaa
     4081 tgcaccgcgc caatgcgggc gcaggccagc atcgcaaccg cggcttccgg caccatcggc
     4141 atataaatcg ccaccacatc acctttttta atgcccagct cgagcagggt attggcgaag
     4201 cggcacacgt cgcggtgcag ctctttatag ctgatatgtt tgctctggct ggcgtcgtcg
     4261 ccttcccaga tgatggcggt gcgatcgccg ttttcttgca gatggcggtc aaggcagttt
     4321 gccgccagat tcagcgtgcc gtcctcgtac catttaatgg acacattacc gggggcaaag
     4381 gaggtatttt tcaccttctg gtacggtttg atccagtcga gaatttttcc ctgttcgccc
     4441 cagaaggtat caggtacgtt aatagattgt tgatacatcg cctcgtactg ctgagggttt
     4501 atcaggcaac ggtctgcgat gttggcagga atggtgtgtt tgtgaatttg gctcatgctt
     4561 ttgttctcct tgtaggatgt taataatatg tggcataagc gttaaatgta ggggtattgg
     4621 cagttttgtt tagtatttgg gcgacagatc acgcaaaagt agaattgtgc aaataaacgg
     4681 cagggtaatt tttgaaggtc accaacaaaa gttgattaat tctttgagga acatgcagtt
     4741 atgcatgctg ttgaaaaaga ggaagatact gactaactct aaagtggtat tttacatgca
     4801 cttacaattg attaaagaca acattcacag tgtggttata tgttacacat aggggcgagc
     4861 aatgtcatga cagtgtaggt gcggttactg tcgtgaaaac aataaaaacc gccattgcaa
     4921 caatggcgca attcggatga agcccctatg acaaggataa aaataaacgc acgccgtatc
     4981 ttcagcttat tgattccttt tttctttttc acttctgttc acgctgaaca aacggcagct
     5041 cccgcaaaac ctgtaactgt ggaagcgaag aatgaaacct ttgccccgca gcatcccgat
     5101 caatatctct cctggaaagc cacctcggaa cagtcagagc gtgttgacgc cctggcggaa
     5161 gatccacggc tggtgatcct gtgggcgggg tatcccttct cgcgcgatta caacaagccg
     5221 cgtggacatg cctttgctgt gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa
     5281 aacgctgaag atggtccgct accgatggcg tgctggagtt gtaaaagccc ggatgtggcg
     5341 cgtctgatcc agaaagacgg cgaagatggc tacttccacg gtaagtgggc gcgcggcggc
     5401 ccggaaatcg tcaacaactt aggttgtgcc gactgccata acaccgcctc accagagttc
     5461 gccaaaggca aaccggagtt aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc
     5521 attggtaaac cttttgagaa agccggacgt ttcgaccagc aatcgatggt ttgcggtcag
     5581 tgccatgtgg agtattactt cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac
     5641 ggcatgaaag tcgaaaatat ggagcagtat tacgacaaaa ttgccttctc tgactggact
     5701 aactccctgt cgaaaacgcc aatgctgaaa gcgcagcacc cggaatatga aacctggaca
     5761 gcgggcattc acggtaaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag
     5821 aacgccgaag gcaaactcta caccgaccat aaaattggta atccgtttga taacttcgcc
     5881 cagacttgtg cgaactgcca tacccaggac aaagctgctt tgcaaaaagt ggtcgcggaa
     5941 cgtaagcagt cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac
     6001 ttcgaagcga aagcagcgct ggatgcaggc gcgacggaag ccgaaatgaa gccgattcag
     6061 gacgatatcc gtcatgccca gtggcgttgg gatctggcga tcgcttccca cggtattcat
     6121 atgcacgcac cggaagaagg tctacggatg ctcggtacgg cgatggataa agcggcggat
     6181 gcacgcacca aactggcgcg cctgctggcg accaaaggca tcacccatga aatccagatc
     6241 ccggatatct cgaccaaaga gaaagcccag caggccattg gcctgaacat ggaacaaatc
     6301 aaggccgaga agcaggactt catcaaaacg gtgatcccgc agtgggaaga acaggcacgt
     6361 aaaaatggtc tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg
     6421 gagtgaatat gagcgtatta cgttcgttat taactgccgg ggtgctggcg tcgggcttgt
     6481 tgtggagcct gaacggaatt accgctaccc ctgcggcgca ggcatctgat gatcgctacg
     6541 aagttaccca gcagcgtaac ccggatgccg cctgtctgga ctgtcataaa ccagataccg
     6601 aaggtatgca tggaaaacat gcttccgtta tcaacccgaa taacaaactg ccggtcacct
     6661 gcaccaactg tcacggccag ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc
     6721 gctttaacga gccgatgtac aaggttggag agcagaacag cgtctgtatg tcctgtcatc
     6781 tgccggaaca gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt
     6841 gcgccagctg ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag
     6901 gacggattaa gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc
     6961 cggcgtctgt tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac
     7021 cggcgtcggc gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt
     7081 gaatatcaat ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac
     7141 cgcctgtatg gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac
     7201 gattattcgt agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa
     7261 gtcttgccag cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt
     7321 tcgtgatgcc gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta
     7381 ctgcatcgcc gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga
     7441 caaatgcgat ttctgccgta aaaccaattt gcaggccggt aagctgcccg cgtgcgttga
     7501 ggcttgcccg acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc
     7561 gcaactgctg cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa
     7621 gctgtaccgc gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt
     7681 cattttgaat cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt
     7741 tctgccggtc tggtgacgct ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt
     7801 gcagacagta cgttgctgcg caccacgctg attgtcgggc cgggcgcggt gatcctcggt
     7861 ctgttgatcc tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac
     7921 tacagtttta cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta catggtggtg
     7981 ctggtgctgt ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg
     8041 ttgccgaagc tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga
     8101 ctggaaacat tgatgctggt gttggcggtg ttgttggggg cttataccgg ctttctgctg
     8161 tcggcgctga aatcgtatcc gttcctcaat aacccgatcc tgccggtgct gttcctcttc
     8221 tccggcatct cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt
     8281 aacccgcatt ccacggaagc gcagtttgta caccgtatgg aaatccccgt ggtatggggt
     8341 gaaatcttcc tgctggtggc attttttgtc ggtctggcgc tgggcgatga cggtaaagtg
     8401 cgtgcgctgg tggcggcatt aggtggcggt ttctggacgt ggtggttctg gcttggtgtc
     8461 gccgggctgg ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc
     8521 attcctgccg tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt
     8581 ttcattctct acgccggaca gttgacggtg gcgtaagcca gaaaagaggt ggtttctgga
     8641 cgtattcctt cctgaagtcg gttttctggc gttgttgtta agtctcgggg tcaacgtgtt
     8701 gaccccgttg acggcctttg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac
     8761 ttgcatcggc attctggcgc agttcgcgct cctgctgctc gcctttggcg tactgacgta
     8821 ttgttttctc atcagcgatt tctcggtcat ttatgtcgcg caacatagct atagcctgct
     8881 gtcgtgggaa ctcaagctgg cagcggtgtg gggcggccat gaaggttcgc tgctgctttg
     8941 ggtgctgctg ctttccgcct ggagcgcgct gtttgcctgg cattatcggc agcaaaccga
     9001 tccgctattt ccgctgacgt tagccgtttt atctctcatg ctcgccgcac tgctactgtt
     9061 tgtggtgctg tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga
     9121 tctcaatccg atgctgcaac atcccggtct tatctttcat ccaccgctgc tttaccttgg
     9181 ctatggcggt ttgatggtag cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt
     9241 tgatggtgcc tgcgccagaa tttgttggcg ctgggcacta cctggctgga gcgcattaac
     9301 ggcggggatc atcctcggtt cctggtgggc ctattgcgaa ctgggctggg gcggctggtg
     9361 gttctgggat ccggtggaaa acgcctcttt attaccctgg ctttctgcca ctgcgctgct
     9421 gcacagtttg tccctgacac gccagcggga gattttccgc cactggtcgc tgttgctggc
     9481 gatagtaact ctgatgctgt cgctactggg taccttaatt gtccgttctg gcattctggt
     9541 ttcggttcat gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc
     9601 actgattagc cttgcgtctc tggctctgta tggctggcga gcgcgggacg gtggtgcggt
     9661 ggtgcgtttt tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg
     9721 cgcagtgcta ctgatcgtgc tggtgggaac gctttatccg atgatttacg gcctgctggg
     9781 ctggggacgc ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac cgtttggcct
     9841 gttgatgctg gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc
     9901 ggcgctggta gctcatgcgg gcgtgctgtt atttgccgcg gggatcgtgg tttccagcgt
     9961 cagccgtcag gagatcagcc tgaatttaca gccgggtcag caggtgacgc tggcaggata
    10021 caccttccgt tttgagcgcc tcgatctgca agccaaaggc aattacacca gcgaaaaagc
    10081 gatagtggca ctgtttgacc atcagcaacg cattggtgaa ctgatgccgg agcggcgttt
    10141 ttacgaagca cgtcgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga
    10201 ctggtatgcg gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc gcttgtatgt
    10261 acaaagcggt gtgcgctgga tctggggggg aggattgttg atgattgcgg gcgcattgtt
    10321 aagcggatgg cgggggagga agcgcgatga ataaaggact tctcacgcta ctgctgttat
    10381 ttacctgttt tgcgcgcgct caggtcgtag acacctggca attcgccaat ccgcaacaac
    10441 agcaacaggc gttaaatatt gccagccagt tacgttgtcc gcagtgccag aatcaaaact
    10501 tactggaatc caacgcgccg gtggcagtca gtatgcgcca tcaggtttac agcatggtgg
    10561 cggaggggaa gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg
    10621 ttcgctataa cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt
    10681 tgttacggct gatggcactg atcctctggc gagtgagggc gaagcgatga aagagcccaa
    10741 aataccggtg aaaatgctga caacactcac gattttgatg gtatttctct gtgtcggcag
    10801 ttatctgtta agtccaaaat ggcaggcggt acatgcggaa tatcagcgtc agcgcgatcc
    10861 gctacatcag tttgccagcc agcaaacccc ggaagcgcag cttcaggcat tgcaggataa
    10921 aatccgcgct aatccacaaa acagcgaaca atgggcgtta ctgggcgagt actatctgtg
    10981 gcaaaacgat tacagcaatt cgctgctggc gtaccgtcag gcgttgcaac tacgtggtga
    11041 gaacgctgaa ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat
    11101 gaccgcccag actcgcgcaa tgatcgacaa agccctcgcg ctggactcta acgaaatcac
    11161 cgctctgatg ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga
    11221 actatggcaa aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga
    11281 gtcgattaat atggcgaaat tgttgcagcg gagatcagat taatcatctg gtgatgaaga
    11341 ttatgttatt gagttgtata aaaaataatc gaatgaacat atgccaaaaa taatcaccaa
    11401 tcagtattat tgcagattaa acaaataaaa atctttccat aacaaatggt tattcattaa
    11461 tcctgccata aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg
    11521 ttgcgcaaca taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa
    11581 cgcaacacaa ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg
    11641 cgctccattg aggaagttat tctgatgaaa agtataaaat tcagcctggc ctggcagatt
    11701 ctgtttgcta tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc
    11761 cgcgactggc tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc
    11821 aaaatgattg ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt
    11881 gatgccaaac agctcgggcg tattggcgcg aaaaccatta tttacttcga ggtgatcacc
    11941 accgtcgcca tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg
    12001 gatatgtcgc agctggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta
    12061 caaagcagtt cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg
    12121 gcgtcgatgg cgaaaggcga aatgctgccg atcatcttct tctcggtgct gtttggtctg
    12181 gggctttcct ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc
    12241 tctgaaacca tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt
    12301 gcgctgattg cggtgacggt ggctaacttt ggtttctctt ctctgtggcc actggcgaaa
    12361 ctggtgctgc tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg
    12421 gcgcgcctgt gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt
    12481 ctggcgtact ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa
    12541 gcctacggag caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac
    12601 cttgatggtt cgacgctgta tcaaagtatt gccgctattt tcatcgctca gctgtatggc
    12661 attgacctgt ccatctggca ggaaatcatt ctggtgctga cgctgatggt gacctcgaaa
    12721 gggattgctg gcgtgccggg cgtgtcgttt gtggtgctgc tggcaacgct gggtagcgta
    12781 ggtattccgc tggaaggcct ggcgtttatt gctggtgttg accgtatcct cgacatggcg
    12841 cgtactgcgc tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa
    12901 cacaaatttg accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat
    12961 aaaactgcgg atcaataatt gaagattgcc ggggttatcc acccggcaat gtgtggatgc
    13021 ctgatgcgac gctggtgcgt cttatcaggc ctaccttaac cgttggttaa tttttcaaac
    13081 tcttcacacc cggtatcaaa cccttccata cagctcaggt tcaaccagtg cagcgccttt
    13141 tgtttgttct tctcgataaa accttcctca ccgtttaaga acatcattcc cgcccagtac
    13201 tcggaataac cggttcggga aattgcagag ctgcgtttga aataccaggt tgccttgtca
    13261 tcgtcggcct taatgcccac gccgttggcg taaatcaaac caagcagcat ttgggcatcg
    13321 acggcggagt cattctccag atcttccgag gcgttttcta acagcgaaat cgcctttgga
    13381 taatccggtt tacccgcttg agtatttacc agaatatgcg ccagcgttac ttcaccttct
    13441 ttactgcccg cttgcgccgc tttctctgca agaacttttg cttgtggata gtccagactg
    13501 accggattgg tgattttgat ctgcgccagc agcgcgcagg catcagcatc accgctgtcg
    13561 gcggctttct gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg
    13621 gcaagaaaat attgtgcgcg tcggtccccg gcctctgctg cctttaaata ctggctgcca
    13681 ggctcggagt cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc gataattttt
    13741 ttcattttga taatacgatt gggtagggag taaccagtat aaagagttcg aatggataaa
    13801 aaaacagcct ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc
    13861 aggcgagttt tcaacttgtt gtactcgtca atcacgtact gctcggcggc gcgctgatcg
    13921 gcgatcggct cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt
    13981 aagttttcgg taaccagctc gttacaggca ccaatccacc actggtaggt catgtaaatc
    14041 gcccctttgt tcggacgatc gctgacctgc gcacgggtga taattttgcc tttacgcgag
    14101 tgcacccaaa ccaatgcctc atcttcaata cccagacgtt tggcgtcttc ggtattgatt
    14161 tgtgcgtagc caggttcatc agccagcgcc gccagtgccg cacagttacc ggtcatcgaa
    14221 cggcaagagt agtgaccaac ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg
    14281 agtttgtcga ttggcgctac ccagtcgcag gtgaagaact gtgccagacc gttcggggta
    14341 tcaaacttct ctttaaacag ataagaagtc ccctgatcgg catctgaagt atcgcggcaa
    14401 ggccactgaa tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg
    14461 cacagatgac gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat cggataaccc
    14521 atacgggtgg cgatttcact gatgatttgc cagtccgttt tcagatccca tttcggttca
    14581 actgctttga agaaacgctg gaagccacgg tcagccgcag taaacacgcc ttcatgctcg
    14641 ccccacgacg ttgacggcaa aatgacatcc gccgccgacg cggttttggt cataaagatg
    14701 tcctgaacga taaccagttc cagatcttca aaggctttac gtactgccga cagctccgcg
    14761 tcagtttgca gcggatcttc gcccataatg tacgcggcac gcacttcgcc atgcgctgcg
    14821 cggtgcggca gctcgctgat gcgataaccg gtatgagcag gcaggctttc cacgccccag
    14881 gctttggcga atttctcgcg gttagccgga tctttcacgt actggtatcc cggatacgta
    14941 tccggcagcg cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt
    15001 acacccgcat gcggcttacc gaggttaccg gtcagcatcg cgaggctggt cagagaacgc
    15061 acggtttcca cgccctggta gaactgggtt acacccatgc cccacaggat ggcggcgctt
    15121 ttcgcctggg catacatccg tgccgcctga cgaatctcac tggcgctgac gccggtgata
    15181 tcttcaaccg actccggcgt gtagccttca acgattttac gatactcttc aaagccttct
    15241 gtacgggaag cgacgaacgc tttgtcgtac agattttctt caataatgac atggcccatc
    15301 gcattcaaca gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc
    15361 gcggtttcaa ttttgcgcgg atcgcagacg ataattttcg ccccgttacg tttagcgtta
    15421 attacgtgat tcgccacgat tgggtgggaa tccgccgggt tgtacccgaa aacgaacact
    15481 aaatcggtat tatcaatttc gttaatagca ttgctcattg cgccattacc gaccgattgg
    15541 tgcagacctg caaccgatgg gccgtgtcag acacgagcgc agcagtcaac gttattggta
    15601 ccaataacgg cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc cgtaccacgc
    15661 gaggagccgg tcgtctggat ggcatccgga ccgtacttct ctttgatggc gctcaggcgc
    15721 tcggcaacgt aattcagtgc ctcatcccag gaaacaggtt cgagtttgcc gccacgctgg
    15781 cgacggatca tgggggtttt caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc
    15841 cagccataat aacccttcag acacagggta ccctggttgg ttttcccctg cgctgcctcc
    15901 gcccggacga ttttgccgtt atcgacgacc aggttgattt tgcaacctga tgcgcaatag
    15961 gggcaaaccg tgacgacttt tttcatcggt ctcgctccag ttaatcaaat cacgcatacg
    16021 cgctctcgac tacagtatgc atcttttatg ccacatttta tttggggtca ttccctgata
    16081 ttacgggcac tatttattca aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg
    16141 acgaaacgaa ataccgcgtg acagccatca cgcggcagac attttatttt ttctcgacga
    16201 cgggagccgc ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc
    16261 ggcggctgtc cagcatcagt aatgacatct cttcggcaag tactggcaac cgggcttcag
    16321 tggcctgtaa gcggctggtt aagccgcgct gataggcggc ctcggcagcg cgctgggtga
    16381 agcgcgttgc ttccacgcgt tcagcctgca tttctcgctc gtcgttgagc gtttgcagac
    16441 gcgtgccgtt gacggcaacg tcacgcaccg cgttcagtac tgactggttg taacgttcaa
    16501 tcatcatgtt gctggcggcg cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa
    16561 acagcggcaa tttcagaccc gggatgaagt tgaactggcg actggttttt ttgaataagg
    16621 tatccaggtg gatggcatca aggccgaaaa acgctttgat atcaaagctc gggtagaaca
    16681 gcgcccgcgc ggaatccacc tgatctaatg acgcctgaac ataccagcgc atggcttgca
    16741 gatccgggcg tctggcgagc aactcataag agagtgtcgc cggaatgccg gtctggactc
    16801 gtggtaatgc caccggtttg atctccggca tatcgctggc ccccgcgcca atcaatgcac
    16861 gcagagattc tcgcgtttcg gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg
    16921 ccagaatctg tgcccgcgcg ccctggaaag gcacttgtgc ttccagaccg tgcgccactt
    16981 tactctggtg cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct aacagatcga
    17041 gcatctgata gctggcctgc atactgtaat aaagctgcgc tacgcccgtg gtcagcgata
    17101 gctctactgc tgcggtttct gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg
    17161 ctgagcgatg cacaccccac aaatcaagat ccagtccggc aaacaaagct actgtggctt
    17221 ccgtatagta cggcccgtcc atacccagtg cgggcgcatc catcgcataa gggcttaaaa
    17281 agccgttcgc cgagacgcgt tggcggttga gcatccctaa cgctgcgacc tgtaattgtg
    17341 aaccggcatc taacaaatcg gcctgggact gcgctttttc ttcccgcagt ttcgcttcgg
    17401 cgagggtgtg tgaaccactt agcgtccgtt ggagcagcgc atccagctgc gggtcattga
    17461 gttgtttcca ccactgcgcc tgcggccagc cggagctggc aagatgaata tcgtcggcca
    17521 gtttgatttg ttccggtttg agctgttgat gaggtgcaga atccttacgt accagggcac
    17581 agccggaaat cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac
    17641 gattgatcat tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt
    17701 ccagtctggc aagcgggcga aaagctggca gacgtgttgc tcctgtttta ataaggtggg
    17761 caggatggcc tgcgaggcgg gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc
    17821 ggttgccaga ccggcagcgt atttctccag ggcgtcggca aactgagctg ctctgtccgg
    17881 ctgtagtgcg ttatccagcg cctgcgccga gttccaggtg gcatcccagg cgtgaagaat
    17941 atcgcggccc tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct
    18001 gtccagttga cgctccagcg ccacgcgttg gcacatctct tcacaggcat taaacgccgc
    18061 atgcagaccg atacgaattt gcagataagt gcgcagagcc gtgacttcct gctggcgtgg
    18121 gatccgcatt actttactta acatacccag cgtgccagcc agtttttgcg gcagtgtgcg
    18181 cgcttcactt tcaggccaga caaaggtgta aatcaccgcg gacaccacgg taccaatgat
    18241 gatacccagg gcgcgatcgc gaatttccac caggtcgtac actgggccaa aaacgttttc
    18301 gagcgtggcg agcgcgaagg tgaccaccat ctgtgtgccg atataagaag agcgttcaga
    18361 gctggtggca atccatgcgc ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac
    18421 aatattgtcc agccagggca tgaccagtag cgtgaataac agcgccagaa tcgcgccgca
    18481 aaaggccccg ccaaaacgca gcaccatctt ctggtacgac gaaccgacat ttgggttagc
    18541 gacgatcacg catgtcagca tacaggtgtg aatgccttcc cagtccacgc cgctgtaaaa
    18601 ggtgtaacag atcaaacagg cgagcagcgt ttttaccgcg tagcgcatat agtctggatt
    18661 ggtaaaagca tcggcggcca ttgatggcgg tttggctgcg ggcgttggcg gcgtattcgg
    18721 gtccatctga cccagttgta acaacgtctg gcagatattc tccaggttac attcccgtgc
    18781 cgccatcgct tcactttcac tgatccgcca gtcgctttgc cagcactgac cttcagcaac
    18841 ggcatgctgc agcttattga tttctgaggc taatttttgc cgaaattcaa taattgcctg
    18901 agaatcagca aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt
    18961 tgccacgcag ctttgccacc atgcgctttg agttcgccag ttggcatcgt ccgcgagaca
    19021 aaagacattc agtttttgca gcgccagcgc ctctctttca atccgcgttt cgggtagcgg
    19081 tgcgaggctg tccgtcaggt ggctaatggc atcatcaagc cgatcattaa gcgcctgatg
    19141 catttgcgaa atggcacgac tgggaaacca cagcacgccg attaacgtca tcagcaaggt
    19201 tggataaagg ccaacaacga tacaccacag cgttaagcgc acgaccactt ccggatagtc
    19261 gagcatggcg gggaaggttt gcccgtaaat ggcgacaatg gcgactgcga aaaagaccag
    19321 tcccagccga tgggtgcgca tcaaaaacat acagcccatc aggatcggcc cggcgatgaa
    19381 caatcggatc aacggttcgc cgtatgacca tttgtagatc aaaaacaggc tgccgatctc
    19441 cagcatcgtg gcgaccacaa ataagatcgc gacaaatttg gtgtaaaacg cgttcgactg
    19501 aataccgtaa aacagcactg ccagcgataa cgccacgaaa gggatctcaa aggtcatcga
    19561 gatcagaatc accagcaggc agcctaccca gagttgcacg gtctgcggca cgcggcctgg
    19621 tcgccgctcg cttaactctt catgaaagaa cgccagcagc ctgaccaccg gtaatggcag
    19681 ggagttgagc gcgctcatta ttgcggctca agattagcga ctgccgaagc gccgatgcgg
    19741 aacatttccg ggtcaggttt atcgaccatg attttgaccg gaaaacgctg ggcaacgcgg
    19801 acccagttaa tagagcgaga aactttcggc aggccgccca gcaccaggcc gccgtcatcc
    19861 ggtagcacgc cgtagccaat cgaatccact ttaccctcga aggttttgcc gctgtcactc
    19921 atcaggcgaa tcgttgcggg tgtacctgag cgaatatttt tcagatcggt ttcgcggaag
    19981 ttggcgatca cgtaccagtg acgagtgtcg attagggtaa aaatagggcg catggcagaa
    20041 gcaaattgcc cgacggaggt tttgagggaa atgacccggc catcaaacgg cgcgcgaacg
    20101 gtcgccattt ccagatgcag tttggtcagg gcaatatccg cttcgaccgc cgcacgctgg
    20161 gcaactaatg catccacgcc gctgacggcg ctggcggctg actgcgcctg caacaataca
    20221 gcattaagat cggcttctgc agcgcgctgc gcggtcctcg cacggtcaac gtcttccgct
    20281 gagacaaaac cttctttcag taatggctcg gtgcggcgta atgtatctgt ggcctgtttc
    20341 gcggcggcac gggctttttc taccgtggcg ttaaccgagt cggcaccaaa ctgttgcgcg
    20401 tcaacgctac gctgggtgag cataatttgc ttatccagcg ccgcgaggga ggcttcagct
    20461 ttcgccagat tggcttcgta cgggcgcggg tcaatgcgga acagcaaatc gccctgtttg
    20521 actgcctggt tgtcggtgac cgccagttct acaatgcgac cgctgacttc cggcaccaca
    20581 tcaatggtat ctgctgacgc gtaagcgtca ttagttgatg gcgcactgtc tacgcgccag
    20641 ataacgaaaa caagggcaac cagcgccaac gcaaccacta acagagcagg gaatttactg
    20701 cgaggagctt ttttcggcgt actttccatg agcgcgtctc aaatagatta gaaaaatgcc
    20761 agccagaaca gcatggcgta gagagcaaaa agggcggtat aaataattcc gacaaatgcc
    20821 agattgatat ttgcccgctg aataactcgc ctcgtgatga gcatcaaaat aaggctggca
    20881 atggcgcaga aaaaccagct gggataataa gcaccgatca ccgggattgc cggagataat
    20941 gagcatcccg aaaccatgaa gacgggaatt atccaggctg tttttttgag ttgcatcgcc
    21001 atgcgagaga gaactgttgg catattaaat acatcctgtc tgctgcgaac aatatctgta
    21061 gtgaatatca atatccattg aaggcgtcag tatggctatt ttctcgatga ataaaaatag
    21121 ctttattcag cca
//
LOCUS       DAZFKM010000083        20940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFKM010000083 DAZFKM010000000
VERSION     DAZFKM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..839
                     /gene="csgD"
                     /locus_tag="ACHKAY_003192"
     CDS             189..839
                     /gene="csgD"
                     /locus_tag="ACHKAY_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO5701832.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            844..1233
                     /gene="csgE"
                     /locus_tag="ACHKAY_003193"
     CDS             844..1233
                     /gene="csgE"
                     /locus_tag="ACHKAY_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO5701833.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1258..1674
                     /gene="csgF"
                     /locus_tag="ACHKAY_003194"
     CDS             1258..1674
                     /gene="csgF"
                     /locus_tag="ACHKAY_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO5701834.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1701..2534
                     /gene="csgG"
                     /locus_tag="ACHKAY_003195"
     CDS             1701..2534
                     /gene="csgG"
                     /locus_tag="ACHKAY_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO5701835.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2598..3089)
                     /gene="ycdZ"
                     /locus_tag="ACHKAY_003196"
     CDS             complement(2598..3089)
                     /gene="ycdZ"
                     /locus_tag="ACHKAY_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO5701836.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3191..3745)
                     /gene="ycdY"
                     /locus_tag="ACHKAY_003197"
     CDS             complement(3191..3745)
                     /gene="ycdY"
                     /locus_tag="ACHKAY_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO5701837.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3769..4506)
                     /gene="ycdX"
                     /locus_tag="ACHKAY_003198"
     CDS             complement(3769..4506)
                     /gene="ycdX"
                     /locus_tag="ACHKAY_003198"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO5701838.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4561..5499)
                     /gene="ghrA"
                     /locus_tag="ACHKAY_003199"
     CDS             complement(4561..5499)
                     /gene="ghrA"
                     /locus_tag="ACHKAY_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO5701839.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5733..5820
                     /locus_tag="ACHKAY_003200"
     tRNA            5733..5820
                     /locus_tag="ACHKAY_003200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5767..5769,aa:Ser,seq:gga)
     gene            complement(5970..6812)
                     /locus_tag="ACHKAY_003201"
     CDS             complement(5970..6812)
                     /locus_tag="ACHKAY_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5701840.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6897..7094)
                     /locus_tag="ACHKAY_003202"
     CDS             complement(6897..7094)
                     /locus_tag="ACHKAY_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5701841.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7106..7597)
                     /locus_tag="ACHKAY_003203"
     CDS             complement(7106..7597)
                     /locus_tag="ACHKAY_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5701842.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7594..7968)
                     /locus_tag="ACHKAY_003204"
     CDS             complement(7594..7968)
                     /locus_tag="ACHKAY_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5701843.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8015..8389)
                     /locus_tag="ACHKAY_003205"
     CDS             complement(8015..8389)
                     /locus_tag="ACHKAY_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5701844.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8552..8773)
                     /gene="yeeT"
                     /locus_tag="ACHKAY_003206"
     CDS             complement(8552..8773)
                     /gene="yeeT"
                     /locus_tag="ACHKAY_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5701845.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8836..9312)
                     /gene="yeeS"
                     /locus_tag="ACHKAY_003207"
     CDS             complement(8836..9312)
                     /gene="yeeS"
                     /locus_tag="ACHKAY_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO5701846.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9328..9813)
                     /locus_tag="ACHKAY_003208"
     CDS             complement(9328..9813)
                     /locus_tag="ACHKAY_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5701847.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9904..10722)
                     /locus_tag="ACHKAY_003209"
     CDS             complement(9904..10722)
                     /locus_tag="ACHKAY_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5701848.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(11062..12132)
                     /locus_tag="ACHKAY_003210"
     CDS             complement(11062..12132)
                     /locus_tag="ACHKAY_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFO5701849.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGTSIG
                     AFNGAIIAASPDLSEAAVRLEALWEHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            complement(12129..13034)
                     /locus_tag="ACHKAY_003211"
     CDS             complement(12129..13034)
                     /locus_tag="ACHKAY_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO5701850.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            complement(13031..15416)
                     /locus_tag="ACHKAY_003212"
                     /pseudo
     CDS             complement(13031..15416)
                     /locus_tag="ACHKAY_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(15527..18376)
                     /gene="cah"
                     /locus_tag="ACHKAY_003213"
     CDS             complement(15527..18376)
                     /gene="cah"
                     /locus_tag="ACHKAY_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO5701851.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            complement(18748..19620)
                     /locus_tag="ACHKAY_003214"
     CDS             complement(18748..19620)
                     /locus_tag="ACHKAY_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5701852.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            19949..20131
                     /locus_tag="ACHKAY_003215"
     CDS             19949..20131
                     /locus_tag="ACHKAY_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701853.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            complement(20360..20434)
                     /locus_tag="ACHKAY_003216"
                     /pseudo
     CDS             complement(20360..20434)
                     /locus_tag="ACHKAY_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(20431..20856)
                     /locus_tag="ACHKAY_003217"
     CDS             complement(20431..20856)
                     /locus_tag="ACHKAY_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5701854.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
BASE COUNT         4968 a         5526 c         5200 g         5246 t
ORIGIN      
        1 ttaacacttg atttaagatt tgtaatggct agattgaaat cagatgtaat ccattagttt
       61 tatattttac ccatttaggg ctgatttatt actacacaca gcagtgcaac atctgtcagt
      121 acttctggtg cttctatttt agaggcagct gtcaggtgtg cgatcaataa aaaaagcggg
      181 gtttcatcat gtttaatgaa gtccatagta ttcatggtca tacattattg ttgatcacta
      241 aaccgtcttt gcaagcgaca gctctcttgc agcaccttaa acaatcgctg gcaattacag
      301 gaaaattaca taatattcaa cgttctctgg acgatatatc ttcaggctct attattcttc
      361 tggatatgat ggaagcagat aaaaagctta tccattattg gcaggatact ttgagcagga
      421 aaaacaacaa tatcaaaata ttgttgctaa atacgcctga agattacccg taccgcgaca
      481 ttgaaaactg gcctcatatc aacggcgttt tttatgccat ggaggatcaa gaacgtgttg
      541 tcaatgggtt gcaaggcgtc ctgcgcggcg aatgctactt tacgcaaaag cttgccagtt
      601 acctgattac acattctggt aactatcgtt ataacagcac ggaatcagcc ctccttactc
      661 atcgggaaaa agagatcctg aataagctgc gtatcggcgc gtctaataac gagatcgctc
      721 gttcgttgtt catcagcgaa aatacggtta aaacgcatct ttataatctt ttcaagaaga
      781 tagccgtaaa aaaccggaca caagcggttt cctgggcaaa cgataacctc aggcgataaa
      841 gccatgaaac gttatttacg ctggattgtg gcggcagaat ttctgttcgc cgcagggaat
      901 cttcacgccg ttgaggtaga agtcccggga ttgttaactg accatactgt ttcatctatt
      961 ggccatgatt tttaccgagc ctttagtgat aaatgggaaa gtgactatac gggtaactta
     1021 acgattaatg aaaggcccag tgcacgatgg ggaagctgga tcactataac ggtcaatcag
     1081 gacgttattt tccagacttt tttatttccg ttgaaaagag acttcgagaa aactgtcgtc
     1141 tttgcactga ttcaaactga agaagcacta aatcgtcgcc agataaatca ggcgttatta
     1201 agtacgggcg atttggcgca tgatgaattc taaataaaaa attgttcgga ggctgcaatg
     1261 cgtgtcaaac atgcagtagt tctactcatg cttatttcgc cattaagttg ggctggaacc
     1321 atgactttcc agttccgtaa tccaaacttt ggtggtaacc caaataatgg cgctttttta
     1381 ttaaatagcg ctcaggccca aaactcttat aaagatccga gctataacga tgactttggt
     1441 attgaaacac cctcagcgtt agataacttt actcaggcca tccagtcaca aattttaggt
     1501 gggctactgt cgaatattaa taccggtaaa ccgggccgca tggtgaccaa cgattatatt
     1561 gttgatattg ctaaccgcga tggtcaattg cagttgaacg tgacagatcg taaaaccgga
     1621 caaacctcga ccatccaggt ttcgggttta caaaataact caaccgattt ttaagcccca
     1681 gcttcataag gaaaataatc atgcagcgct tatttctttt ggttgccgtc atgttactga
     1741 gcggatgctt aaccgccccg cctaaagaag ccgccagacc gacattaatg cctcgagctc
     1801 agagctacaa agatttgacc catctgccag cgccgacggg taagatcttt gtttcggtat
     1861 acaacattca ggacgaaacc gggcaattta aaccctaccc ggcaagtaac ttctccactg
     1921 ctgttccgca aagcgccacg gcaatgctgg tcacggcatt gaaagattct cgctggttta
     1981 taccgctgga gcgccagggc ttacaaaacc tgcttaacga gcgcaagatt attcgtgcgg
     2041 cacaagaaaa cggcacggtt gccattaata accgaatccc gctgcaatct ttaacggcgg
     2101 caaatattat ggttgaaggt tcgattatcg gttatgaaag caacgttaaa tcgggcggtg
     2161 ttggggcaag atattttggg atcggtgcag acacgcaata ccagctcgat cagattgccg
     2221 tgaacctgcg cgtcgtcaat gtgagtaccg gtgagatcct ctcctcggtg aacaccagta
     2281 agacgatact ttcctatgaa gttcaggccg gggttttccg ctttattgac taccagcgct
     2341 tgctggaagg ggaagtgggt tacacctcaa acgaacccgt tatgctgtgt ctgatgtcgg
     2401 ctatcgaaac aggggtcatt ttcctgatta atgatggtat cgaccgtggt ctgtgggatt
     2461 tgcagaacaa agcagaacgg cagaacgaca ttctggtgaa ataccgccat atgtcggttc
     2521 caccggaatc ctgacagata aaaaagccgg aattatcatc cggcatttta ctgtttaagc
     2581 gaaagcgtga gtgcccctca cgctttattt ttgatttcct gctcacggtg agccgtcttt
     2641 gcactacgcg ccgccagcca caggccgctg tttttcattg cgtaaccaaa taccagccca
     2701 agcgccagcg aaggtaacac cagtttccag tcaccctgcc cggcaaacgt cgcgcatgcg
     2761 cctataaacg tccccggaac aaatgaaagc agcagctgtt tggcctgaat acacatcaga
     2821 aacgcgacaa taccggttat gacataaccg agaatttcca gatgtggtgc cagtgcacta
     2881 ccgtaaataa tgaccatcgc ccacaccacg ccactaagca gggttgcagc ggagatcgcc
     2941 agccctttca gcccaccttg cgggcaggca aagtaggccg tacagcccag gaagcccgcc
     3001 cagcttagta agcccaggga aacagccacc catccccaga taccggagag aatgcctgtt
     3061 gtgattgcaa tagagagaag tatgttcatg ccgcgcatga tagcagaaaa cgcggccatg
     3121 aatgagatcg agcacacatt ttatgcaaca acaaattatc tgttgcagtt atgagtgacg
     3181 atgatcacat ttactcttca gaatcttctt ccagctcgtc ccacattgca ctaatggcat
     3241 cacgggttag cggtgccatg gtgcgccaga aaggcgtggt tgcatgggcc tccactttgc
     3301 cgaggaacgc accacaccag ggtaacagat actcactgaa cagtgtttcc agtgcttcgc
     3361 tctcatcttc cgttgactga tcttccagcc aggaagccgc aagcagcaat gtgccgatgt
     3421 gatcggctgg cgtatccgcc agtggcatcc ctcgttcgga aagaaaagcg cgcacttccg
     3481 cttccgttgc gtcctcaacc catgcgctac gatatggcgg cacggcacat tcatcgccga
     3541 taaacaacgc attgtaatcg gcagagactt gcgccatatc acagcttttc tgtaaacgcg
     3601 tcagtaactc atcctgctcc agtggccagt tcgcagccag tttcccctca cgaatcaggg
     3661 taaacagcgg caccagtaaa ggatcttgcg gttggcggta atagagcgaa cccagcacac
     3721 gacagaggat agaaaactcg ttcattaata tatttccatt actagtaatt aaaggtctgc
     3781 aaattccgca atcggtgcca taccgcgaga ttcaaggaag ttcagtaagc ggcgcggcga
     3841 aacattcaaa atgcgctctg gcggaaaatc taccgcgtcg aggattttaa gacactcttc
     3901 aaattccccc atggtaaacg cggtgtgaga atccgagcct aatgccaccc aacctccagc
     3961 atcacgtacc gctgcggcta ccgcacgaca gttgtcttca ctgcccttac gtgagtgtaa
     4021 aaatgaagaa ttattgattt ccagcgccac ctgatgtttc gcggctgctt cagcaacggc
     4081 tttcacatct atttcatatt tgggatttcc gggatggctt attatatgca cattgccgct
     4141 ggcgatagtg gcgatcatcg cttgtgtatt ggtcgctttg tcatgtggtg caaaaaccgg
     4201 ctcatgaaaa ccggcaataa tcagatccag cgagtcaaac attttaccgc tgcaatcaat
     4261 ttcaccatca acatttttaa tgttagcttc gatgccgcgc aggatcccta ccccatcaac
     4321 cactcgcggc cagatacgca tgttaatgaa gtgccagtga tgcggagcat cttccatatc
     4381 cgggccatga tcggtgatcg caaaaagttt aatgcccttt tgtttggcct gggcaatgta
     4441 atcactcaat gtgctatatg catgtgtgct ggcaacggta tgcatatgaa ggtcgacggg
     4501 atacataact ctctcctgta ttcaattccc gccaaggata gcaggaatcc tgatgcttta
     4561 ttagtagccg cgtgcgcggt cgacttgccc gcagaccctc tccccttttt cgagctgggc
     4621 aatggtgcga gaaatgtact ccacagcttc agcgggacgg gtaatcgcgg cgacatgtgg
     4681 tgttatcgtc acgcgtggat gttgccagag cggactttca ggcggtaagg gttcacgatt
     4741 aaaaacatcc aacattgcgc ctttaacttt gccgctatcc agcgccgcga gcaggtcatc
     4801 ttccacaaca tgaacaccac gcgccaggtt gaggagatac gcgccatccg gtaatttttc
     4861 gagtaattgt tgattaataa tgccgacggt ttcaggggta ttcggtaaca aattaatcaa
     4921 tacccgacat tggctcagaa atgcagacag ttcttcccgt ccggcaaagc tttgcacgcc
     4981 aggccacgat ttacgggttc gactccagca acgcagcgga aagcgccagg tttgcagact
     5041 ctgagcaact ttactgccca gtacgcctgc gcccaaaatg ccgatggtaa aatcttcccg
     5101 atgatattca ggcagcggtt gccaatgcga actattttgc tggatgcgat aatcgtcaaa
     5161 acgtcgaaac caatgcagca cctgactgac agcatattcc tgcatttgct cgcccatacc
     5221 ggtatcttcc aggcgaaaaa gtggaacaga agggttcagc atttcagggt gtgcctgtag
     5281 cttgctcaaa atagaatcaa caccggcccc gagtgcgaac accgctttaa gatcgcgccc
     5341 tgccagcatt tcaacaggag gatgccagac taaagcataa tcagcagagt cattatctcc
     5401 gcttttccac gctctgactc ttgcctgagg aatagctttg cgcagtgcct caatccacca
     5461 ttgggtatcg aacgttgggt gataaaagat gatatccata ctgactcccg aaaagcgttg
     5521 tgcgaaattt attcgcactt atcgttatga tctacaaagg ccaccagcat aacaaatccg
     5581 tggtcggtgg caaaaaaagc agatttcgct tattaaaacc acacattgat tgaagtttga
     5641 ataaacgcgc gattttttca aaaagtttgt tgacctcagg tcatgatttc cctaaattag
     5701 cgcccgttcc agcaagacag gaacgacaat ttggtgaggt gtccgagtgg ctgaaggagc
     5761 acgcctggaa agtgtgtata cggcaacgta tcgggggttc gaatcccccc ctcaccgcca
     5821 gattataagc gcatgttatt gtttaacatg cgctttttta ttttcccttc cagacagtat
     5881 gccatcgggt atgccatctg aaaccggttt atatgccccg cgatcatatc ttatcggaca
     5941 atttccgatc tttgagcata ctggcaaagc tatactgcca gcatatcagg atagtgtttt
     6001 gcgataatat cattcagtct gtcaaccagt tctggttttc tgaacgtgat atgcgcggag
     6061 cccttcttaa agtatctgat gctgaacatc tcatcttcat agctctcttt accctgaaca
     6121 gactgaatgt gatcatccag acgaagggtg atgtttcccc ggttgtcggg gattggttta
     6181 ccgctgaaca ggtgcaacat tctttccaga tcggcgagtc ggtcagcctg ttgtccggtg
     6241 ataagatgaa atccccaccg gtcccatctc accagattgt tgacgataat tttactgcca
     6301 aatttacagg ggcaattggt tttgtaattc cagttcagcc ctctgaagac gttgatcacc
     6361 cctcgctcaa acacctcatc cttattctgg tgcagttgtt caaatgtgct cagaatgttc
     6421 gcttcgctga tttccggaaa attatcgtac tccagtgacc ggtaccatgt atcacgggcc
     6481 tgcgtatcca ttaatgacag catcccggaa cgttgcatca ggtcacgcca gatttcgcga
     6541 tcaagattcc gggtaatggc tttcattgct gtttcgggtt tctccatcag ccagcagccg
     6601 cagcgaaaat cctgtttcat ggcccagtcc ggggcagttt tacctccgat gctttccgtc
     6661 agcaccgaga tttcagccag ttgtcggata agtgtgtcga tttgcaacag tgtactcttt
     6721 cgtacagcca gaatgtggcg aatgctggtt gagcagataa cgtccgtatg cccgttgacc
     6781 acttccggac gggagattgt gatatctgac ataatcgata ttcctttttg cagataaatg
     6841 aaaacgccag ccctgtgcag gctggcgtca gaggaatgaa gtctggtgag tgagcttcac
     6901 cggttctggt gcagaagctg cagatgacgc agggtacgga catcctcccg ggcctgttcg
     6961 atacagggca acagtgctgc tgaatccgtt ttatcctcat cgttgtcaaa gataattccc
     7021 gatccgcagt cgatattgtc ctgcagccac gcaatcagaa tatccagcgc tgtttccgtg
     7081 gttaatgatt tcatgttgtt cctggttacc agtcgaaagt gggtaaaccc ggcaggcatt
     7141 cagcgctggc atccagatga atgagactga cgccataacg ccggatgagt gtgacgacca
     7201 gacgacggaa cgtttttgac agccccaggc gtttaagccg cagaaccggg tatgaccacg
     7261 catcggtacg taacagatat ccgctacctg taaaatgaat ccattctgat tcaccgaagt
     7321 cactggtctg gtgtgacagc gagtacaacc aggcgttgtc cgtttccgtg atatgtgcgg
     7381 tactgcagcg tatgccggta agggtagcaa atggtggcgt gcattctgct gaggtctggt
     7441 cagattcatc aacaacacga agggagtagc cgttatcgca gaccatgtta atcagttcag
     7501 cgagctcaat accatcgacg tcaacgacaa tgcgccccat gttcagtgcc tgtacgttaa
     7561 cgctgtcggc ttccagtgtc agggccagtt tcatcatttc gcctccggat acttacccaa
     7621 ggtaatgtta tttaccgttc tgtaattgtc gcgggtcatc aggccggtcg cccggcgagc
     7681 ccggaggata tcaatactgt ttattaactg agagcgggga caggtgctga atcccggctg
     7741 gtcggtacgc accagcgcgt atttttccac gagaaaatta actgcatcac acagtgaaat
     7801 gcctgcctca atatgctgct caatcacacg ttcatcggca aacggtgtgt cattcagtgt
     7861 gaggccataa tgctggtcca gcagtcggat gagcagtgtc tgccagatgg tgacgggaga
     7921 cgggcagcga gatacctccc gtacatgtgt gtcggataat gttttcatct taagttctct
     7981 gtaactggta gtgctaaagg tgagagcttc ggggttatga ggtggttgcg agtgctggtg
     8041 tcggataaac agccagataa acgtaaccac aggagccgag ggtgtcggct tcgcaggtca
     8101 gcccttttgc attcagcgtg acggtatgct gatatcgggg attcagttca ccgctggtga
     8161 gcatgagttc cagttgtttc atcagcagcg gaaaggcctg gtccagatgg tatgcatctg
     8221 cgtcgctgaa gcggcctctg ataccggcgc ggtcggcaag gtaatgcagg cggttaccct
     8281 cttgcaccag acgagcgcca aaacagggcc tcactgtgca gggaagcccc caccaggggc
     8341 ggtcgtgatt gtcatcggga tgagttatcc cggagagttt gtctgacacg ataaaatcct
     8401 ctctgaaaag tggttaagaa tggtttaacg atgacaggtc agccggcgcg ctgaccggtt
     8461 tcggtgaaga tgtatgcatt gatgagaatg gcctcatcca cagcggcgtc tgaggtcatc
     8521 tggtcgtact cgtttcccgg gtgacggaac atcagttcag ggtgacactc atcagccgga
     8581 catacgggcc aaaactgtcc ttacggcgtt ctgcaaacac ggcaagaacg ccgggaatat
     8641 cctgcacttc acgaccagta tacgcttcag cactgccgtg ccagcgatac ttaccggtac
     8701 agaacggaaa aagacgggat gtcggatgtt gttggtgaat acgcatggct tcaccacggg
     8761 tgataatttt cataatggga tacctctgaa gacagaagat aaaagtgaaa acaggtgtga
     8821 tgtggttgtg aagggttaaa gcagaccatg ttcggcaaag gagaaaacct ggttgccacc
     8881 gactatcaga tggtccggca cccggatatc caccaggccc agtgcctgta ccagacgttc
     8941 ggtgataagc cggtctgcct tactgggtgt gacttcaccg gacgggtgat tgtgcgccag
     9001 caccacggca gcggcattgt ggtacagggc gcgtttaatc acttcccggg gatgaacttc
     9061 cgtacggttg atggtgccgg tgaagagggt ttcaccggca atcagctgat tctggttgtt
     9121 cagatacagc acccggaact cttcccgctc cagtcccgcc atgttcagaa tcagccattc
     9181 ccgtgccgca cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt
     9241 ttgcagggcc cgcagaatga gactgcgctc gccgggcgtc atctctccgg gcagaaagga
     9301 aagctgctgc attgtccctg cctccattca gtcaatgatg cgcataatgg cgctgcattc
     9361 cggatgctgc agggcgtaat cccgcagccg gtaatagtgg accgtcatgg cataacactc
     9421 cgtacgacag gcatgatgac tgtacgtcat cagacaggca gcaataccgg cggcttccgg
     9481 gctcatttca gcgcggttgc cgttcatggc attgaacagt acccatgttt cgtcatcatc
     9541 gtcatccggt tcgggtgcca taaatgcccc gccgttgttc agggtgtaca gattccagat
     9601 accaccgcag tagtcttcgc aaagacggtc catccagccg aagacacggg gctccagggt
     9661 cacccactgt ggaatgaggc caaaatgctg cggccagaag ctgatgcgct gttcatcagg
     9721 gacgggagtg gcaaccagct gaggtgggtt attccctgat gcagtggtta cggaaacaga
     9781 aggtgtggtg gaattatgcg aaacggttgt catgaggtta ttccttataa aaagtaaatg
     9841 aatggaagaa gccccggggc aggaacagat gtgagtcagg actgcacttt cagtgaaacg
     9901 gcatcagcgc atgctctcca gcagcgtttc tgccatcacc cacaatgcgc gattgagctt
     9961 aatgtcggta tcaatgctgt gaatggcccg ggtatggata cgttttcctt ttgcactgcg
    10021 accggaaatc ccgcctttca gcatattctc ctggatggtc tgataagcac tccacaggtc
    10081 cttaccgtaa tcctcccggc gtcgtggtgt cagaatgtcg gcggtggtga cgggctgatg
    10141 ttcgtcacca taacggtaag tcagtgccgc ctgtgccagc gcctggcgtg ccggtggcgg
    10201 caggaccagc gactgcatgg catcacgctt ctcctctatc cggtcaaaaa cgcccaccac
    10261 ctcgtaagcc ccttcaatga ctctgtccac cacatttccc cggtgtggaa cacgcaattc
    10321 tcccagagac tgaccgcaga ccagtccatt ggtacaaatg gcacgaaaat atccgggtaa
    10381 catctggtag ctggatgaac cgtcatggga gttgagcaga ataatttcag ggacatgctg
    10441 accggttatc tgtccggccc gccgcagacg cagcatgtgt tttgtgtatt cccggcggct
    10501 ctggtcgcgc acgcgggtct ggcaggcaaa gaacggctga aagccttcct gctgcagatt
    10561 ttccaggacg gtgatagtgg gaatgtacgc atagtgttca ctgcgagagg tatgtcggtt
    10621 ttccccaaaa atactcggta catggcgcat cagttcttcg tgtgtcagcg gacgatcacg
    10681 acgtatctgg ttcgcataac caaaacgact ggctaatctc atgatttgct ccttatgaat
    10741 gattaagcga tataaatgaa aaagccgcgt ctcccggaga agacgcggcc tgatgggtga
    10801 aatgaatgag gtttattgtc tgagaagccc ttcactggcg aaccacgtat taagctgtat
    10861 tccggcatcg ccagtgcaac tgaccttcag cattgcggat aacccgccgg aaatatgttc
    10921 actggttgtt tttgtatctt cccccaaaat taaataaatt catcttaacc atcacccaga
    10981 taatatatat ctgaaaatat tttcaataac accagacagg aaaaacacca ccacaggcgg
    11041 tggcgtttct ttggggcgtc tttatttaat ccgggccatc gcgtttctca gtgccaaatc
    11101 tgcctcttcc gttatatcca ggcttttctg caataccgcc tctgaccggg tcagtgcctg
    11161 acgtgctgcc agcggtttcc ggatatgctc catcgccagc atcgtgtctt catagccctg
    11221 ctgacgccag gcgtcggtat gtgccgatgt aaaactgagc agaccgccgc tgttgccacc
    11281 atcacctgca tatttcagcc ttttccgggg acggatctca aggattgtgg tgtccggaaa
    11341 agcctggcga tcccataaag aaccgtcact cagatgcgtc acaatcacca tattgcatcc
    11401 ggcatcgacc agaggcgtca caggggtatt tccctgcata tttcgccatc ctcccatacc
    11461 accatcaccg aacattgtcc cctgaacctc acggggacgg aacagcaggg gcagcgcggc
    11521 tgacgcaagc agagcctctt tctgctgtcc gcggggcagg ctctggatat gctgaaaaac
    11581 ggcgtttttc gtggttccga cacccagttc agcacgaatg cagtcaataa tatcctgcat
    11641 gcccccttct gtggggtaca gcgacacata cagcggtagc ccgtctgcca gagcatcagt
    11701 atcaagataa tgatccatca gcgctgtcag gggctcatct gacaacaacg gctgagccat
    11761 cagattgtca aacccgttga ttgtcgatat atggcgaaga agcgtcgtaa gcagtgctcc
    11821 tgcacgtccg gggatctggc agaggttcat tgcctggaac aattttttca gcaatgaaaa
    11881 gtaaaccaat ctgtttaccg acagcacctg attattcccc agatgctccc agagcgcctc
    11941 caggcgtacg gcagcttctg acagatcggg agaggccgcg ataatggcac cattgaatgc
    12001 gccaatgctg gtccctgaaa ccatgctgat ctgtgtacca cactctgcca gtgccttaac
    12061 cactcccgcc tgataagcgc ccaccgcccc gccaccggac agcaccagcc ccgttttcat
    12121 ttctgtactc atgcagcccc cttctgaaat atttcatcga ccatactttc agagaggcgt
    12181 tctgcagagc agaggcgagg catgcgaacc ggtaagtgtt ctgcagcctg cgtggttgac
    12241 cagatcaccg gatgctgctc ttcactgcac cagtctccca gccagttaat cgcgtcatgc
    12301 cactcgttat ctgcctgctc ccggccctga ccaccaagcc agtccagagt gttcagacgc
    12361 acggttgcac taccgccact ctcctgtgcc atctgcgtta atgctttatg tctgagaata
    12421 tccagtagct ggttaacctg gcctgtttcg ccctgacgta tcacatcacc aaacgcgccc
    12481 cagcgaagct cttcaagtgc cacataacaa cgtccggcag gggaaaatga cgcatatcgc
    12541 ccggcgctcc accatgagaa tatctgctca agatgtaact gcgcccgctg aacctggtca
    12601 acacggtgga gtttttcttc aagatgattc agtttatggt gaacctgatc ggccagggtg
    12661 agtaactgct cccgcgtatc tgcggaataa tgcgccagcc tggctgtatc actgaccagt
    12721 gaactgaccc tgtcgtttac ccgggtaatc gcgtagtttg tggtggcaag tgacgttgtc
    12781 atctccgtca gccagcgcag ggacgcctca acgccctgtg caagcgacgc attgatggca
    12841 ttctgccgtg cagcgccttc gccggtcagc ccctctttta aacgccggaa gaacgtgcgc
    12901 tctccggccg tgcggatatg ctcctgtgca acatcgattc cgtttgcgaa atcaaccaca
    12961 aatttttcag gaagacaggt gagtacctga catacccggt tatcagcaat ttgttgaata
    13021 aatggtgaag tcacagcatt gtctccagct cttccctcag cgccttactg tcagtaatga
    13081 gctcaggaac cctttcattt aatgcctgaa gtcgcccggt aagccgttcc tgcgcctgct
    13141 gatcgcgttt atgatcttca gagctctgaa tcagcgtgtt acgcagttgt tcaactttct
    13201 ccctgaatgc gcagaaaaag gcatcgattt cctggcggac gggctggtta atatcctgct
    13261 caatggatgc ctgcagctcc ccgaaatatg cccgggtcag tgacataact tcctctctgg
    13321 ccttgttgat attgatcacg ctgcgactca catcctcgtc atagttttcc cagcccaggt
    13381 cactggtgcc aaaccatcga caaacggttc cccatacgcc actctgccga cggcgacggg
    13441 tgacggtctc cgttctttcc tccataagcg cactcagatt tatttttacc gcgagccggg
    13501 tctgtaactg gctgactgcc ggaaaactga tttttacggt aaagccctca tcctccaggc
    13561 gggcattgat gcgaccggca atgttttctg tggcgtacag ggccgtgcca cgaaaacctg
    13621 tttcaatccc gctgacgatt ttctccagct caggccggaa ctgtgcttca gcctcacggt
    13681 gcaggctggt cacggtcgat tcgatttgcg tcatcagttc aagggctgcc ctgcgatcac
    13741 tgaattttat ttcggggctg tccggatcaa agtcccgcat cctgtcatgc tgatttccgg
    13801 ttccgaaaat atcgtgaaag aaagcgaatg cgtttctctc ccggggctgc gctgaatttt
    13861 cttcctccag catttgctgt tctttgcgtt taccgcttct gaaatagtcg ttcagctcct
    13921 cctccagtga ggcacagacc ttcgtcagta attccccggt tttggtattg atgtcctcca
    13981 tggccattct gacatcaccg gtcacctgat tctgacattc ctcaatctcc tggatatccg
    14041 ccagcaggct ggtaatatgc gcctgtagtg actgaatgct ttgctgcagc ccctgattga
    14101 cgtaatgaca gatattcgct gacattttct gcattctgca tcagttttgc ggcggcagag
    14161 tcaaccgcca gtgccgccgc tctggaatgg gagctctgta tgacttccgt tatcagttga
    14221 tcgatcagag atatattcca gagtttttct gcctttttat ttaccttagt gctgtctttc
    14281 cagtcgtcct cttccaccat aggtccgaaa gcttccctga taaaatcatc gacccaggct
    14341 tcatcaaccg gaagcgcacc gttttttcgt aactcatgaa gcgcacggtt agccaggtac
    14401 gcctgacgtg atgagccggg ataaacgcgg gaggcgggca gcacatcgct gttcagcatt
    14461 gctggcacct tctggcgtac ggcatccgca ccatcaccat tgcgatcttt ttcatcgaat
    14521 ttattgacca gcacaaacag acgaccggct gagacgtcag caatggcatt aagctcttta
    14581 cgaacctcct catcggcttt cgagttcatc tgggtgtaat ccatcaccgc cagcaccgca
    14641 gaggctttct gtagttgatc ccgcatcatc atttccatct gtggttgtcc ggcttcatta
    14701 gggccggggg tatccagcag tgtgagtgtt ccctgacatg catccattcc caccaggtgg
    14761 ctgaacgcca cgtcgatcac cggtagtttc tgcacttctg cgtattcgtc aaagggaaat
    14821 tcagtcccca ttgccgcagc aagacgaacc aggtcattta acgatgccag tccggtaaag
    14881 atttcgtctt ccccgtggta ttcattttta agccagacat catccgccag aatatccagt
    14941 agttcgcggg tatccccggt ttgctgcagg gactgtgcga cttgctgccc tgccggggtg
    15001 gcgagttttt cctgcagtgt gattaataaa ttgcggacgg gctgaatatg ttccagatgg
    15061 aggaccggtt ccgtttttcc gggaacgtgg cggatcaatg tcggtaccga ggtcatgggg
    15121 cggttacggt tgggcagaat ttcctgcccc acaatggcgt tgatggtggt tgatttgccc
    15181 gccttcatcg tgccgaccac agccagcacc atctccctgc gggcggtttt gatttgttcg
    15241 ccctccagct cctcaattct tttcagcgct ttccgtttat caaaaagcag cccgttttcg
    15301 ttcttactgt cagataacac atccggctca tcaaccattt gccggagcag attgatattc
    15361 agttgcaaaa gccggtcggc ttcatcacaa agcagggcga tgttcttttc gtgcatcatt
    15421 cacctttata aattcaataa ttcagaactg cggtgaacag caatgagcgt cctttcccat
    15481 tactgcatga attttcctga aatcgtattt ccgggatacc gtataatcag aaagtcatat
    15541 tcagcgtagc ctgaccgtta tagccttcag cgctgctgcc gctgacgctg tgggcataac
    15601 cggcctgaac gcccagggtg atattttccc ggatacgggc ttccagtccg gcctgcaggt
    15661 ccagtgacgt gccattccgg gacggtgaga acgtcatgtt actgccggct gcggctgtcc
    15721 ccatgctcat gtcaccccgg gagctgaagg tgcggataac agaaggctgt acccaccagt
    15781 tcaccggcag ttcactcaca ctgtgttttg cactgtcgcg cagggtgtca cgggatgagg
    15841 tgccttcacc aaaggtcata tcgttgtggc tgcccagacg gaaaccggca cgcacatgtt
    15901 gtgcactgcc atgcccgaac ttcacataac cggcgttatc ctggccgtca tccagggaga
    15961 gtccctgcca ggtgtactgc agttgtggct ccagcatcag attgtcagtg atactgaagg
    16021 gcagaccggt ttccagtgag cccagccagc cccagccccg ggcgcggaag tcgttattgt
    16081 ccgatgacgc tttcatgctg tgacgggttc cctgggccac aatgtcagcc cacaggccgg
    16141 aggatgtgtg taccagattc aggtatccgc ccagactgcc ggcatcatcc cggaccgtgc
    16201 cggcgcggga accgtcatca tccttaacat caacggaaga atggcctgca gcaccataca
    16261 cccctgtcgt cagagacata ccggcaacct ctgttctgag caggtcaccc tccagacgga
    16321 cgaagccata gctgccgctg ctttccggcg tggctccacg ggcaataccg ccgttgttat
    16381 cgtgaccgag atgaccgccc tgaatgctga gacggacgct gttattttca ccgtttacac
    16441 cggtctgatg gctgcgggag cctgccagaa tccggtcata gtccattgcc tgtgtcaaca
    16501 tggatgtata cagggggact tcagcacgat aagcattttc actgcgcagg taccagtctt
    16561 catcgctgtc acggttcagg gtgtagttaa aggcgccggc ctgaagcggg cgactcaggg
    16621 caaacgcacc ttcttctgtg gtggcgccat tctgtgcatc cacaacccgg ataccctgtc
    16681 cggtggttgc caccccgagg ttgctgtttc cgacatttgt aaacgcaagc caggttttgc
    16741 cggttgcctg accaccatta atcaccagct ggtcagaggc attgctgcca tcaaggcgaa
    16801 cacgcatatt gatggtgccg ccctggccgg tgaggttcgt ggtggtcagt ttatggaacg
    16861 ttaccgggga gttacttttt gcaacagcac ggctcagcgc ggcattcggt gtcgtctgat
    16921 tatcaaagcg aatctcccct gcgttaacaa tatcgccggt actgaccaca tcaccattca
    16981 gtaccatact ggcgccttta ctgagctgtg ttctgccact cagacttccc ccggcagcca
    17041 gcgtcagtgt tccacgatgt ccgacagtgg tgttgccagc ctgtcccccg gcattaaccg
    17101 taaagctgcc gccattttcc agcagcaggc cgctggcctg accggatgtg ccatcaatac
    17161 tgaacttacc gctggcattg gtcccctcaa cagtggcacc actgtctgca atcagggcac
    17221 caccggatgt tatggtgaca cttgtcgcct taccgccggc agacactgcc agggtaccgc
    17281 cgtcatccac tagggtattc tgtgctgaat ggctctccag tacatccaga cgaccgccgg
    17341 attccagaac aacaccgtca gccttaccgt tttccaccgt gaaattcccc agacggtttg
    17401 tgccgatgac agttgcagca gtactggtaa ccagtgcacc gcccgtgttc tgggtgactg
    17461 cagtggcttc cccgccggca tgtaccctca gttcaccgtt ctgatttatg gtggtgttac
    17521 cggcagcgcc acctgcctta acaacctgcc agcccccctc gttaattacc gtgttcagcg
    17581 caagtccgtc cctgtgcaca tactggtaac cgccattcag tgtggtattc agggcccttc
    17641 cgtgtaccga ctggtcacca cctgcgtaaa tgagagtgtc acgggctagc ccggaaaata
    17701 agataatctg ccgtccattt ttgttgatgg tggtggattc tgctgttccc tggaccttct
    17761 gacccgtata cgagttgtca gaatcagggc cgccttctgc gccggtgttg atgatggttc
    17821 ctgttgccag cccgccactt ttaacgctct ggtaaccgtc gcggttgata attgtaccgg
    17881 tggcaacccc gccctcatgc acccactgct cgcctctgtt attcagtgtg gtgttcaccg
    17941 ccagtccgtt caggctctgt cccccgccgt cacgaataac cgtatcactg gctgttcccc
    18001 cctccagcac gacctgacga ccatttgtgg tgacagtggt gtttccggct atcccgccat
    18061 tctgtatcca ttgcccaccg gtgttttctt cactgtccgg ccccagttcc agcccggtac
    18121 tgatggtcat tccgttggcc gtaccgaaga caatctggtt gtcatgattt gtcagtgttc
    18181 catcgttcac ggtttctccc gcctgtacaa ccttgtcagc agccagtgcc gggactgatg
    18241 tgacagcagc aagagacagc gcaaccgcca caccggcgcg ttttccccgt gagcgggcca
    18301 gttcggaggc caccaccagg gtgcccgtaa tgtgattcca taccagcctg tagctggtgt
    18361 tcagatgtcg tttcatcagc ttttccttac agagggtgaa taaaaaagcc ggtacccaca
    18421 aaagtggata ccggcaggta caaaacagag ttatcgggca cagcgatacg tcccgggcgt
    18481 aaacgctctc ccccttcatc agacagagaa gtatcagtct gttgtgtgca attccgttct
    18541 gcatgatgaa gagattcagc tgccttttcg caggccatta accactataa acgatcgata
    18601 taaacgatcg atacctgttt tatcgatcgt tttattctat taacagtaga gcagtcacgc
    18661 aagagaaaca ggaggggcat gaggtgtttg tatcatgttt tcagttggtt aacgcaatgt
    18721 aatcagacat ttcccctggc agactgttca gaagaaaatc cagttccata ccgcgcgggc
    18781 aacagagacc accgtgtcac ggacggcacg cagaactgtg cgtgcaacag aggcaacaca
    18841 gacgctctcc accgtgtcaa atatccggtc caccgcaccg gtaaactgtt cacgggcctg
    18901 cccccggaca gattccgtgc acagctcgtc ctgcaggcgg gtcatcaggg gactggcggc
    18961 atggtcggga agcgctgtca tgagcgcact gaccagcgta tccagttccc agccggtgcg
    19021 ggccgatacg gcaacaaccg gatgtacggg ccggaacagc cggaataccg catctgtttt
    19081 ttcgcgaatg ttctgttcct gtgcgggaga aggctgaata cctgccatat cccattcatg
    19141 gcagggctct gttttgtcgg cctgcgtcac cacaaacagc acctgctgat gtccccggtg
    19201 cagtatgtgt cgccagaaat actcatccac agacagggca cggtcatcgg ctttaatcag
    19261 ccacagtacc aagtccagtt caggcagaat gtcacggtac agggcttcat actctgcatc
    19321 cctgtcccgg ctctcgccca ccccgggcag gtcagtgata accatgctgt gaccatggcc
    19381 actcagacgg aagcgccgca cttcccgggt gccggcgtga acatcactga ccggggtgac
    19441 ctccccctga aacagtgcat tacagagtga ggatttaccg gccccgcttt tacccataat
    19501 gccaatcacg ggttcgtgac tggtgagttt gcgcagatgt tccaggatgt gacgggaaag
    19561 cgagtaaggc agggaggaga gcggtttttc aattgcctca atggcatcag acggattcat
    19621 acatgtattc ccggtgaaaa aagacaaaac cccgcgcacc ggaaaggtgg cgggggattc
    19681 acaacgataa tatctgtctg aaattattta ggatgcaggt atcttctgat aatcatacat
    19741 cccgaatttt gttcttaata gttgagtagt ggtatttggt ttccctgaga gaatataaag
    19801 gtgaatcatg ctgcattgag tttgattcaa tttgtactga gacaagtatc ttcatgatta
    19861 taatcaaaag ttgggtggat tggtctggcg ctcatcattt tttcgaataa actcactaat
    19921 tattgccccc cccaaaggag atgatttcat gtataattgt ggtgaatcat attcagaata
    19981 cgacatatca ttatctgacg atgaaaacta tgacgatgac tattatcgtg aacgcccgtg
    20041 tccgggagaa gttttcggct atgataatac tgaggataat gatgaggcgg catctgatta
    20101 tgcattaaaa ataaatgaaa actctgatta atacgggggt agctgataat aagacaatca
    20161 atgacagata ttaatgaata aacataatca ggcgctcaat acttggttta atctgggcgc
    20221 ctgattaaaa tctgaggaaa catataataa ttttcatcaa aatagtattt atgtactaac
    20281 taagctggat ttgaacctct atttctgggt atttgttatc acttaatcca ttactgctcc
    20341 tccttcagca tatgtcgtat ccgttgaagg atttacgcat atcggtaacg tcggcaacga
    20401 gccagatgcg ggtacctgac gggagtgaga tcatcgtccc ctcccggtca gttcacggat
    20461 caacagcgtg agcagctctg gcgatggatt ttccagcgtc atgttaccgt gacggaactc
    20521 caccttgcag gagccggcac tgacactggt ctgagtggaa gtggataaag acggcgcaat
    20581 ggccgccacc ggttctttct gctcatccgg cgttatttct acaggtaata attcaacgcc
    20641 agtgtcagaa gaggtcgtta cctgaagacg ccgtgatata cgcccttcgt tctgccagag
    20701 cctgagccat ttgaacagga ggttatcatt gatatcgtgt tcccgggcaa tacgggcaac
    20761 agaggctcct ggttgtgatg ccagttccac catacgaagt ttgaattcat tcgaatagtt
    20821 tttacgaggt tcttttcgcc agtcctgtaa ttccatactt agatgtccgt ctatatcaga
    20881 tgggcgtcta agttaccaat tttcgtctga tggctacata cggcggtcag tttacgctta
//
LOCUS       DAZFKM010000084        20591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFKM010000084 DAZFKM010000000
VERSION     DAZFKM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1260)
                     /locus_tag="ACHKAY_003218"
                     /pseudo
     CDS             complement(1..1260)
                     /locus_tag="ACHKAY_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1272..1559)
                     /gene="eutN"
                     /locus_tag="ACHKAY_003219"
     CDS             complement(1272..1559)
                     /gene="eutN"
                     /locus_tag="ACHKAY_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO5701855.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1666..1959)
                     /gene="eutM"
                     /locus_tag="ACHKAY_003220"
     CDS             complement(1666..1959)
                     /gene="eutM"
                     /locus_tag="ACHKAY_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO5701856.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1998..3014)
                     /gene="pta"
                     /locus_tag="ACHKAY_003221"
     CDS             complement(1998..3014)
                     /gene="pta"
                     /locus_tag="ACHKAY_003221"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO5701857.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(3011..3814)
                     /gene="eutT"
                     /locus_tag="ACHKAY_003222"
     CDS             complement(3011..3814)
                     /gene="eutT"
                     /locus_tag="ACHKAY_003222"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO5701858.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(3811..4512)
                     /gene="eutQ"
                     /locus_tag="ACHKAY_003223"
     CDS             complement(3811..4512)
                     /gene="eutQ"
                     /locus_tag="ACHKAY_003223"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO5701859.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4487..4966)
                     /gene="eutP"
                     /locus_tag="ACHKAY_003224"
     CDS             complement(4487..4966)
                     /gene="eutP"
                     /locus_tag="ACHKAY_003224"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO5701860.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4979..5314)
                     /gene="eutS"
                     /locus_tag="ACHKAY_003225"
     CDS             complement(4979..5314)
                     /gene="eutS"
                     /locus_tag="ACHKAY_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO5701861.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5607..7886)
                     /gene="maeB"
                     /locus_tag="ACHKAY_003226"
     CDS             complement(5607..7886)
                     /gene="maeB"
                     /locus_tag="ACHKAY_003226"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO5701862.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8175..9125
                     /gene="tal"
                     /locus_tag="ACHKAY_003227"
     CDS             8175..9125
                     /gene="tal"
                     /locus_tag="ACHKAY_003227"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5701863.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9145..11148
                     /gene="tkt"
                     /locus_tag="ACHKAY_003228"
     CDS             9145..11148
                     /gene="tkt"
                     /locus_tag="ACHKAY_003228"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5701864.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11244..12287)
                     /gene="ypfG"
                     /locus_tag="ACHKAY_003229"
     CDS             complement(11244..12287)
                     /gene="ypfG"
                     /locus_tag="ACHKAY_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO5701865.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12413..12988)
                     /gene="nudK"
                     /locus_tag="ACHKAY_003230"
     CDS             complement(12413..12988)
                     /gene="nudK"
                     /locus_tag="ACHKAY_003230"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO5701866.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(13056..15035)
                     /gene="aegA"
                     /locus_tag="ACHKAY_003231"
     CDS             complement(13056..15035)
                     /gene="aegA"
                     /locus_tag="ACHKAY_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO5701867.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15241..16941
                     /gene="narQ"
                     /locus_tag="ACHKAY_003232"
     CDS             15241..16941
                     /gene="narQ"
                     /locus_tag="ACHKAY_003232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO5701868.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17105..20218
                     /gene="acrD"
                     /locus_tag="ACHKAY_003233"
     CDS             17105..20218
                     /gene="acrD"
                     /locus_tag="ACHKAY_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO5701869.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPLVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20317..20376)
                     /gene="ypfM"
                     /locus_tag="ACHKAY_003234"
     CDS             complement(20317..20376)
                     /gene="ypfM"
                     /locus_tag="ACHKAY_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO5701870.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4708 a         5611 c         5365 g         4907 t
ORIGIN      
        1 gtccgttctt aacgaaaatg ctggtatcaa tggcgttcgc catctggttc atgttttcga
       61 tattgcgcga gtgcattgcc gccgtgtggt ggcaaccgcc ctccagtttc accgccagcg
      121 caatggcatc cgccacgttg gcgacgcgca cgacgggcag caccggcatc atcagttcgg
      181 tcacggcaaa cggatgttct gcagtggttt ccacaaacag caaacgcgtt tcttgcggca
      241 cgttcaggcc gattgccgcc gcgattttcg ccgcatcgcg accaacccag tcacggctga
      301 cggtgccttt tccgcgctcg tcgatatttt tcagcaacac cggttgcagc tgctgcgcct
      361 gttctgcggt cagtttcacc gcgtgctggc cttccatcag acgcatcagt tcatcggcta
      421 cgctatcaac aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagaag
      481 cgcctttgac gatggactga gcggcacggg cgaggtcggc ggtttcatcc accactaccg
      541 gcgggttgcc agcgcctgcg gcaatcagac gtttattggt gtgtttacgc gccgcttcta
      601 ctaccgcttc gccgccggtt accaccagca ggccgatacc cggaaacttg aacaagcgtt
      661 gcgcggtttc gatatccgga tttgccacag taaccagtaa gttttccggc ccacctgcgg
      721 caacaatcgc ctggttgagc agcgtaatcg cccgctggga gacttttttc gccgccggat
      781 gcggggcaaa aatgacgctg ttgcccgcgc aatcaggctg atggcgttgt taattacggt
      841 tgccgccggg ttagtggaag gcgtcaccga agccaccacg ccccagggtg cgttttcaat
      901 tagggtcagg ccgttgtcgc cagtcagcac ttgcggagag aggcactcaa cgcctggtgt
      961 gccgcgcgcc tgagcgacgt tttttgcaaa tttatcttca acgcgcccca tgccggtttc
     1021 actgacggca agttccgcta aatctctggc gtgtttttcg cctgcttcac gaatggcagc
     1081 aatggctaac tggcgcattg ccacgctttt taacccttgc tgggcgactt tggctgccgc
     1141 aacggcgtca tccagggacg cgaaaacgcc catctcatga acggcggcgg acggcgtgtc
     1201 actgctttgc attttcagca gtaccgcttt caccacctgt tcaatatcct gttgattcat
     1261 gatgttctgc cttatttgtg gaaaattacc tgaccgccag acaccacctc atcgacaatg
     1321 ccaatcacgc acagatcgac cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg
     1381 ccactcacca gtaacaccca ctccccggtt cccgcgccaa tattgtcgat ggcgacggcg
     1441 cattgcccgt caggattacc ttgtggatca atcatttcca ccatcagcaa tttgtcatgc
     1501 gccagtccgt gatggcgtac ggtacaaaca atttgtccag tgacgactgc cagtttcata
     1561 cccgcctccg tggcgtattt caggtaaaag ctccccctac cctccgcaga aggtaaaatg
     1621 aaaaaggaga gagcgtgacg cccgaatcga cgtcacacag ggtgattaca ggttgctgct
     1681 atcgcctttc aggccgatcg ggaagacttc ttccaggtcg ccgtgcggac gcgggataac
     1741 gtgtacagat accagctcgc cgatacgctg tgccgctgcg gcacctgcat cggtcgcggc
     1801 tttacaggct gcaacatcac cacgaaccat ggcagtacac aggccgccgc caatctgctt
     1861 aacaccaacc agcttgacgc gcgcagcttt caccatcgcg tcagaagcct caatcagtgc
     1921 aaccaggccc cgggtttcga tcattcctaa tgcttccatt gtgctttcct ctttatcagg
     1981 gtccagaacg ggaccgttca ttcaaccagt gtttgtaaac tgctttcgcg gttcacttct
     2041 gtctgacgcg gcacagctgc caccagcgcc agctcgataa tttcctgcac gctacaacca
     2101 cgagagagat cgtgcatcgg cgcggcaagt ccttgtatca gtggcccgac ggcacgatat
     2161 ccgccgagtc gttgtgcgat tttgtaacca atatttccgg cttccagcga cggaaaaacc
     2221 atcacattgg ccttgccctg tagcgggctg gcaggcgctt tttgcgccgc cacttccggc
     2281 acgaaggcgg cgtcaaactg taactcgcca tccaccacca gctttggtgc gcgctcacgg
     2341 acgatttctg tcgcctgctg gacgttagca acacaggggt gacgggcgct accattgctg
     2401 gaaaacgaca gcatcgccac gcgcggctct tctccggtga tggcgcgcca ggtttcggca
     2461 ctggcaagcg cgatatccgc cagctgcgcc gccgtcggct gtggcaccac gctgcaatcg
     2521 gcaaagccca acgccgggcc gctgtactgt ggcaacatca ggaaaatgga agagagcgtt
     2581 ttacagcctg gctgcaagcc gataatgcgt aatccggcac gcagcacatt tgccgtggaa
     2641 gagaggttgc ccgcgataca gacatccgct ttaccggcgc tgaccattgc tgcggcgaac
     2701 atcagagggt cggtaagttt ttccagcgca tccggcggcg ttttttcgcc cgcgcgggcc
     2761 agccagcgat gagcaaattc ttcccgcatt gcgaggttgc catgcggatc aatcacctgt
     2821 agcccgtcca tcgccacgcc gtgactgagc gcaaactgac gaagttcaaa cggattggcg
     2881 accagaatgg gcgttgccag accttgttga tgtaaatatt gcgcggcttt cagcacacgt
     2941 tggtctaacg catccggaaa aaccactctg gcgggcgctc gcaacgccag ttcacgacaa
     3001 cgttcaataa tcattgcgtt tcccccagtc gttgttggat ctgcttcacc gttagcggct
     3061 gcttcgttac gctcaggatc atcatcacgt agaccgtgct ggaaaggcgg ttcagcgcct
     3121 gcaaaatgtc cgggcgtaat acttcaaagc tgcgggtgat aaacacctgc gccgctacgg
     3181 tttcggtttc ccgcactttg gtacgtagca gattgagcaa cgccgcatcg cgcccgtggc
     3241 tggcttccgg caccagatga tcgtggtcga gatagcgcag cggctggtga gaaagtcgat
     3301 gcagatcttc gtcagaaagc ccgacaatcg cctggcagcc cagcggttca cccagcgcat
     3361 cggcgcgcat aatgttgcca agacgcgaac ggatatccgc cagccacggc tgccacggtt
     3421 ccgccagttc aatttgcagc cacactgcca gcgcaatggt gctgtcgaga accgcgcgaa
     3481 aacccagacg cggatcgctt ttggcgacca ttttttccgc cgacaggtga gtcagcgtat
     3541 ccggcttttt tgccaccggc tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc
     3601 tactggtcag cccatgaacg ggctgcggct gctgctgttc atcgtcaaca aacaggcggc
     3661 cctgctcgtc aataaacttg atgcgcagat ggcggctttc cagtaactcc cgggcagagg
     3721 gcgtcaggcg actgtccgca gggagatgga tctctgcgcc ttcgctgagc gtatggttcg
     3781 ctcttagcca tgcttcggtg atgaaatctt tcatagggat tgccagttag ccggccaggc
     3841 gacatacagg aatttcacgc tggatgtcgt accaaattcg atactggagc ctttcgggat
     3901 aaacatcacg tcacccgctt tggcgatcat ggtttcgcct tcgtggcgaa catgcagctc
     3961 cccttccagc accatgtcga tttcgtcgta gttcagggtc cacgggaaga atgcgttctc
     4021 ccactgcata aaaccagcgg ccatgctgct gccgtcgtct ccggtcacaa gatcagtcaa
     4081 gcccacgcaa tgcggctctg cgccgtcgaa acgaccaaac ttgacgctgc tgccatcaat
     4141 cactttgatg ccgcctttgc cggtcaccga tttgaagctc ggctgcattg cgccctgctc
     4201 cagcgactgt ttttccttca tcactttttc catcagctgt gcgaccaggc tttcggtaaa
     4261 ctggccttcc ggcagctggg caatgatggt ttcgcggatg cgctggcttt cggttttgtc
     4321 ggctggcacg ctggcgggca cggacgccgt taccgggatc gattcatcac attcggtgat
     4381 ggtaaaaccc agtagatccg ccacttcacg ggcttccggg gtaataatgc tggcgcgcag
     4441 aaccacggac attgcctgtt cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat
     4501 gagttttttc acctgcttcc tcctgtttgg tgagagatgc cagataatcc accagttgct
     4561 gcacgctttg cgggtcgtgg ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa
     4621 gcaataactt tcgcgttgcg gcgacgtcag catctggcat gtccgtttta ctgatgacgg
     4681 cgatttgccg tttgctgacg ccaatatcca gcaacccggc aggcaaccga ctttccgggt
     4741 catttgcgcc atggacatag atcagcatgt ctacatcctg tagcgtggta attaaggcgt
     4801 gataccagcg gggatggcta aaatattcac ccggcgtatc aatatcgccc ttatcattaa
     4861 attccacggc ctgtgttttt ctggcgaggg tataattccc ctgcaacgca ttaaataacg
     4921 ttgtttttcc tgccccgacg gtacccacaa acgcaattcg tttcatgaat gcctcgtatt
     4981 aacttttggt catttcacaa agcgtataat ttaataagcg acctaaaccg ctgacggttt
     5041 gtgataacgc ttcttctact gcgcccaccg agccataaat caccaatgca ccgctgaagc
     5101 ggtcgagaaa accgatatgc acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg
     5161 ccgtttcgcc gggagttagc gtcataatgc caattgcgcc cgcatccgga acgccgattt
     5221 tcttcgccag ttcctcaccg ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg
     5281 gcacaaattc ctgaatgatg cgttctttat ccatgagtcg ccgcctgtga aaaactttcg
     5341 tgtatcgtga caatttttcg tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg
     5401 tgaggtagat cactaagaat ggagagatat tcgctgtggt gcataaactt tcatatgcaa
     5461 cgcatgaata taaataaaat aatatcgaga tattatatag actgcttttt ttattattcg
     5521 caaactcagt aataagcttt actaatatat tgcagccaaa ccgcttaccc tgaatattca
     5581 gggtaagcgt gagagttaaa aaagaattac agcggttggg tttgcgcttc aaccacggcc
     5641 agcgccacca tgttgacgat acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt
     5701 ttcgcaacgc ccatcagcac cggaccgaca gtcacacctt ccgagctgga aacgcgcagt
     5761 aagttgtaac taatgcgggc agcttccatg ttcggcatca ccagaatatt ggcagaacct
     5821 ttcaaagggc tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg
     5881 tgcatttcac catcaatcat cagatctggc gcacgatccc tgaccagttc cagcgcctga
     5941 cgcattttgc tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga caacagagca
     6001 acgcgcggct caataccaaa acgacggaca gtttctgccg ccatcaaggt gatctccgcc
     6061 agctcttctg cgtctggttc gtcattgacg taggtatcgg caataaaggt gttaccactc
     6121 ggcagcagca gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata accaaagaca
     6181 tttttcacca cgctaaaatg ttcatgataa tcgcccaccg taccgcagat cattgcatcg
     6241 gcttccccac gctgaaccat gatcgcgccg atcactgtcg ggttactgat cagcgcccgc
     6301 tgcgcctgtt cctgagtgac accgcgacgc ttcatgatct ggaagtattc ggtccagtac
     6361 tctttaaagc gcggatcgga ttcgttattg acgatctcaa aatcaacgcc cgctttgatc
     6421 tgcaagccca gtttctgaat gcgcatttcg atcacgttcg gacgaccgat aaggatcggt
     6481 ttcgccagtc ccagcgttac cagttcctga gtggcatgca gaacgcgcgc ctcttcccct
     6541 tctggcagaa caacgcgctt cggcgctttg cgagcctggg agaaaatcgg cttcataaac
     6601 aggttggttt tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc
     6661 ggacgcgtcg ccacgcccga ctccatcgcg gctttagcga ccgcaggagc gatcttaacg
     6721 atcaagcgcg gatcaaacgg ttttggaatg atgtattccg gaccaaagct cagatcctga
     6781 tcgccatacg ctgaagccac cacttcgctc tgttcggcgt gggccagttc cgcaatcgcg
     6841 cgtaccgccg ccagtttcat ctcttcattg atagccgttg cgccaacatc cagcgcgcca
     6901 cggaagataa acgggaagca aaggacgttg ttgacctggt ttggatagtc agaacgaccg
     6961 gtgcaaatga tggcatccgg acgcacttct ttcgccagcg gcggcagaat ttccggttcc
     7021 gggttcgcca gcgccaggat cattggcgca cgagccattt tcttcaccat ttcctgggtc
     7081 agcactttcg ggccggaaca tccaaggaaa atatccgcgc cttcaatcac atcatcgagg
     7141 gtacgtttgc cgtcatccac caccgcatat gcggctttgg tttccgccat gtttggctca
     7201 cgcccctgat agataacgcc ttttgaatcg caaaccacga tgttatgttt ttgcagaccc
     7261 agcgctacca gcaggttcat acaggcgatt gctgcggcac ccgcgccgga aaccaccatc
     7321 cgcacgtcgg agatgttttt ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg
     7381 atgattgccg tgccgtgctg atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt
     7441 ttctgttcaa tatagaaaca ttctggcgct ttaatgtctt cgaggttgat tccgccgaag
     7501 gtcggttcga gcgcagcgac aacttcaata aatttgtccg ggtcgagttc atcaacttca
     7561 atgtcaaata catcaatccc ggcgaatttc ttaaacagaa cgcccttgcc ttccatcacc
     7621 ggtttgccag ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc gttagagatc
     7681 accgccacca ggttaccgcg cgcggtatat ttgtaggctt ttaacgggtc tttttcgatt
     7741 tcaagacaag gtgcggcaac gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc
     7801 agaggcttgg ttggagaaac ctggattttc cctggaactg gaaattcatg gaaatcaagt
     7861 gcactttgtt ttaactggtc atccatttgg ttgttccttt cacgtaacgt tcacaaataa
     7921 agtgtgtggg caacagcccc tgcccacaac gtggcgcaca ttattaccct gccggagtct
     7981 acagactttg agcaagtcca aactctcacc attaatataa tgttttggta ataatcctat
     8041 aacactgatg ttacctgctt aatccagcaa taccatgcct gtctgctatg cttttttgat
     8101 gcgtttagcg aaatttctca gaagtgtgaa ttaacgcact catctaacac tttacttttc
     8161 aaggagtatt tcctatgaac gagttagacg gcatcaaaca gttcaccact gtcgtggcag
     8221 acagcggcga tattgagtcc attcgccatt atcatcccca ggatgccacc accaatcctt
     8281 cgctgttact caaagctgcc ggattatcac aatatgagca tttaatagac gatgctatcg
     8341 cctggggtaa aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac
     8401 tggcagtcaa tttcggtgct gaaattctca aaatcgtacc cggtcgcgtg tcaacagaag
     8461 ttgatgcacg cctctctttt gataaagaaa agagtattga aaaggcgcgc catctggtgg
     8521 acttgtatca gcaacaaggc gttgagaaat cacgcatttt gatcaaactg gcttcgacct
     8581 gggaaggaat tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc aacctgacgc
     8641 tgctgttttc ttttgcccag gcgcgggcct gtgcagaagc gggcgttttt ctgatttcgc
     8701 cgtttgtcgg gcgtatttat gactggtatc aggcacgcaa gccgatggac ccgtatgtgg
     8761 tggaagaaga tccgggcgtt aaatcggtgc gcaatatcta cgactactat aagcaacacc
     8821 actatgaaac cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa
     8881 ccggctgcga tcgactgact atcgcaccga atttgctgaa ggagctgcag gaaaaagttt
     8941 cgccagtggt acgtaaatta atcccgcctt ctcagacgtt cccacgccca gcacccatga
     9001 gcgaagcgga gttccgttgg gagcacaatc aggatgcgat ggcggtagaa aaactgtctg
     9061 aaggcattcg tctgttcgct gttgatcaac gcaaactgga agatcttctt gccgccaaac
     9121 tataaaccag ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca
     9181 ctcagtatgg atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg
     9241 gctgatattg ccgaagtgct gtggaacgat tttcttaaac ataatcctac cgacccaacc
     9301 tggtatgatc gcgaccgctt tattctttcc aacggtcacg cgtcgatgct gctctacagt
     9361 ttgctacatc tgaccggtta cgacctgccg ctggaagaac tgaagaactt ccgtcagttg
     9421 cattcgaaaa ccccaggcca cccggagatt ggctatacgc caggcgttga aaccaccacc
     9481 ggcccgcttg gacaaggttt ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg
     9541 gcggcgcagt ttaaccagcc agaccatgag atcgtcgatc acttcaccta tgtgtttatg
     9601 ggcgacggct gcctgatgga aggtatttcc cacgaagtct gttcgctggc aggcacgctg
     9661 ggactgggca agctgattgg tttttacgat cacaacggta tttccatcga cggtgaaaca
     9721 gaaggctggt ttaccgacga tacggcaaaa cgttttgaag cctatcactg gcatgtgatc
     9781 catgaaatcg acggtcacga tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc
     9841 atgaaagata agccgtcgct gattatctgc cgtacggtga ttggctttgg ttcgccgaat
     9901 aaagcaggta aggaagaggc gcacggcgca ccactggggg aagaagaagt ggcgctggca
     9961 cggcaaaaac tgggctggca ccatccgcca tttgagatcc ctaaaaagat ttatcacgcc
    10021 tgggatgccc gcgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc
    10081 tataaaaagg ctcatccgca actggcagaa gagtttaccc gtcggatgag cggtggttta
    10141 ccgaaggact gggagaaaac gactcagaaa tatatcaatg agttgcaggc gaatccggcg
    10201 aaaatcgcca cccgtaaggc ttcgcaaaat acgcttaacg cttacggacc gatgctaccg
    10261 gagctgctcg gaggttcagc ggatctggct cccagcaacc tcaccatctg gaaaggctct
    10321 gtgtcgctga aggaagatcc ggcgggcaac tacattcact acggggtgcg tgaatttggc
    10381 atgaccgcta tcgccaacgg cattgcgcac cacggcggct ttgtgccgta taccgcgacg
    10441 ttcctgatgt ttgttgaata cgcccgtaat gccgcgcgga tggcggcact gatgaaagca
    10501 cggcagatta tggtttatac ccacgattct attggcttag gggaagatgg tccgacgcac
    10561 caggccgttg agcaactggc cagtctgcgc ttaacgccga atttcagcac ctggcgaccg
    10621 tgcgatcagg tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca caacgggccg
    10681 acagcactga tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt
    10741 aaagagattg ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc agatattatt
    10801 ctgattgcca ccggttcaga gatggaaatt accctgcaag cggcagagaa attagcagga
    10861 gaaggtcgca atgtacgcgt agtttccctg ccctcgaccg atattttcga cgcccaggat
    10921 gaggaatatc gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca
    10981 ggtattgccg attactggta caagtatgtt ggtctgaaag gggtaattgt cgggatgacg
    11041 ggttacgggg aatctgctcc ggcggataag ctgttcccgt tctttggctt taccgccgag
    11101 aatattgtgg caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg
    11161 gatgctgatt gccggatgcg acgctgacgc gtcttatccg gcctacagcg tcttatccgg
    11221 cctacatgtc cccgcaattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc
    11281 ccatgccagt tatcgcaggt gggttctgcg gcataacgca ccaggcgaaa acgctgaccg
    11341 tcaaagcgcc agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc
    11401 accagttcac gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc
    11461 tgaccgttgt tgaatggcaa acgcaaccga accgggcgcg aggctagtgg ctttttacgc
    11521 gacacaatcc atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc
    11581 agcgctttat catcagtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag
    11641 cagcgcagac cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt
    11701 gagagtggcg tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt
    11761 acgctgagcg gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt
    11821 tgctgagcat caataaacaa caacgccgct tttaagccac tcagagaaat ggtctgattg
    11881 ccgtcgcgta aggtgatcgc cttcccttcc tgaatcacct gcaaaaacgc ggtgatggtt
    11941 gccgtatcat cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc
    12001 aacggctcac catctaacag cagccgtggc gctatctctc cttcagacgc atccggcgac
    12061 ttcaacccgc cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc
    12121 agggtcatca ccagtccatt atgatcgccc gtattgcgcg ccacgcagaa gttttggtta
    12181 ttacaggtga cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac
    12241 gtcggcaaca aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc
    12301 ccagaagttc aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat
    12361 cagatttgtc ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca
    12421 ttaaatgtga cgtttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc
    12481 cggttttgat catctccagc gcctggctga acggcagctc gagcacttca atatcttcat
    12541 cttcaacacc gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt
    12601 ggattagctc agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc
    12661 ccacttcata gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt
    12721 tatccagcag cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg
    12781 tagcgacacg gaactgacga atcagaacca cgctcttttt cttcgcgttg tacaggagga
    12841 tcgtcgcacc attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt
    12901 tgcgcgtgag atcgtaggta atgttgtgca gggtgaaata gttatcggag agaattttgt
    12961 ctttaatgag ggtgatttgt tgcgtcatac cgactccaca gcgcgaaatg aacaattatc
    13021 ttacgctgtg aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc
    13081 cccagccagt caataatccc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc
    13141 agatccgcac cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa
    13201 cggtactggc tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc
    13261 gactccagcc acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct
    13321 ggcatgacaa attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc
    13381 tcgccaagac gcgtgcgcag gaaacgaatc ccgcagacgt gaccttgttc attcagctca
    13441 agcgccaccg gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcctcacc
    13501 tctttcttcg agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc
    13561 gcgccgtggc gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc
    13621 acgacgttaa gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc
    13681 acctgtttag tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca
    13741 ttgggtaaac ccgcgttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat
    13801 tgttccaaaa gcgaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga
    13861 atccccatcg cgctgaagat ttcccgacgg cgtgccagca gggatttatc cagtttgaaa
    13921 gaaggaatgc cgaaagtgag caagccaccg atttctggat ggcgatcgta caccgtcacc
    13981 gccacgccat tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata
    14041 atcgccaccc gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc
    14101 aacgcctgat ctgaaatata gcgttcaata ttgccgatag ttaccgcgcc gtgctcatcg
    14161 cgaatagtac aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc
    14221 ggcagggtgt tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc
    14281 agttcaatcc actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc
    14341 tcgccgcact taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga
    14401 taaatttcat caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc
    14461 gcgggcgttg cctgcatttg ttcgacttta ctcattaccg gcatttcttg cgccgcggta
    14521 ctggcatgcc acggttgatg ttcctgacgc gcggtgcgca agcggcgggg tttcgccatg
    14581 ccggagagtg cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcaacacag
    14641 gcaggaccgt tttcgcgccc cgcacaaagg tcgcatttat gcgccgtggc ttttactttt
    14701 cctgccgcga cgggtgtcag gacgatttgc atcgtaccaa aaggacaggc caccacgcag
    14761 gatttacagc caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt
    14821 gcgccattag ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca
    14881 ctacgttgct gttgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg
    14941 acatgttgct catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga
    15001 cattgctgac tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa
    15061 aaccttattc tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc
    15121 atgtgcccag aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca
    15181 ctgactaaat tgtgtttcag ctaactggaa cattaatgat tttttgtgga gaagacgcgt
    15241 gtgattgtta aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg
    15301 ctgtcgattc tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct
    15361 gaggctatca atattgccgg atcgctacgt atgcagagtt accgcctggg ctacgacttg
    15421 caaagtggca gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca
    15481 ccggtattaa ccaatctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg
    15541 catctgaatg ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg
    15601 tatcaggcca atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag
    15661 cactacgctg aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt
    15721 attttcacgc tggtcttttt tactttgcgc cgtatacgcc atcaggtggt tgccccgctg
    15781 aatcagctgg ttaccgccag tcaacgtatt gaacacgggc agttcgactc gccgccgctg
    15841 gataccagtc tgccgaatga gcttggtctg cttgcaaaaa cctttaacca gatgtcgagc
    15901 gagctgcata aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc
    15961 cacgaggcca agcgtcgtct ggaggtgttg tatcaatgtt cccaggcgct gaacaccagc
    16021 cagattgatg tgcattgttt ccgccatatt ttgcagattg ttcgcgacaa tgaagcggct
    16081 gaatatctgg agttaaatgt cggtgaaaac tggcggatta gcgaagggca accaaacccg
    16141 gaattgccga tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac
    16201 tggcaaaata gtcacgtttc atcatcagaa ccgctgctta acagtgtttc atcgatgctg
    16261 ggacgcggtt tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa
    16321 gaacgtgcaa ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta
    16381 cgtatccagt tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt
    16441 atcatggccg atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg
    16501 actactttcc gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg
    16561 gatacgttac aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg
    16621 gcgctggatg cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat
    16681 gcgatgaagc acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc
    16741 aatcacacgg tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa
    16801 ggtcattatg gtctgaatat catgcgcgaa cgcgcggaac ggctgggtgg gacgctgact
    16861 ttttcacaac cttccggcgg cggcacgtta gtgagtatta gctttcgctc tgcggagggt
    16921 gaggaaagtc agttgatgta atgcctccta ctgaccaaag aatacttgca cttaaggttc
    16981 agtataaaag ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac
    17041 cttgccgcta cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct
    17101 tttaatggcg aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt
    17161 gtgtctgaca gggaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc
    17221 gccaccgaat gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa
    17281 caccgtgacc caggttatcg agcaaaatat gaccggcctc gataatctca tgtatatgtc
    17341 atctcagagc agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga
    17401 tccggacgaa gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc
    17461 gcaggcggtg caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca atattctgac
    17521 cattgctttc gtctctaccg atggttcgat ggataaacag gatattgccg attatgttgc
    17581 cagtaatatt caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg
    17641 ttcgcaatat tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac
    17701 ggctaaagat gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact
    17761 tggtggtaca ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact
    17821 gctgcaaaca ccagaacagt tccgcgatat caccttgcgg gtgaatcaag acggctcaga
    17881 ggtaaggctg ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag
    17941 ccgcttcaat ggtaagccag cctccgggct gggggtaaaa ctggcctccg gcgcaaacga
    18001 aatggcgaca gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt acttcccgca
    18061 cggactggaa tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga
    18121 cgtggtgaaa acgctgctgg aagctatcgc cctggttttc ctcgttatgt atctgttcct
    18181 gcaaaacttc cgcgccacgc tgatacccac tatcgccgtg ccgctggtgt tgatgggaac
    18241 cttctccgta ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt
    18301 actggcgatc ggtctgctgg tggatgacgc aatcgtggtg gtggaaaacg tcgaacgtat
    18361 tatgagtgag gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca
    18421 gggggcactg gtcgggattg cgatggttct ttcggcggta tttgtaccaa tggccttctt
    18481 cggcggcacc accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt
    18541 gctgtcagta ctggtagcga tgatcctcac tccggctctg tgtgccacac tacttaagcc
    18601 actgaaaaaa ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt
    18661 taaccgcaac gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg
    18721 ctggattgtg atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc
    18781 gacgtcgttc ttaccgctgg aagaccgtgg catgtttact acctcggtac aattgcccag
    18841 cggttcaacc caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac
    18901 ccatgaaaaa gacaacatca tgtcggtgtt tgccactgtt ggttctggcc ccgggggtaa
    18961 cgggcaaaac gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa
    19021 gaccggcacc tcgtttgcca ttatcgagcg tgcaacgaag gcgtttaacc aaattaaaga
    19081 agctcgcgtt atcgccagca gcccgccagc aattagcggt cttggtagtt ctgcaggttt
    19141 tgatatggag ttgcaggacc acgctggagc gggtcacgat gcgctgatgg cagcacgtaa
    19201 tcagttgctg gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct
    19261 cgacgacagt ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt
    19321 tgctatcgac gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga
    19381 ctttatggat cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat
    19441 gctgccagat gacattaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt
    19501 ctctgctttc gcgacctcac gctgggaaac aggctcgccg cgtctggaac gctataacgg
    19561 ttattctgcg gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat
    19621 ggatattatg gaatcgttag tgaagcagtt gccaaacggc tttggtctgg agtggacggc
    19681 gatgtcgtat caggagcgac tttccggcgc gcaggctccg gcgctgtacg ccatttcctt
    19741 gctggtggta ttcctgtgtc tggcggcgtt gtatgaaagc tggtcggtgc cgttctcggt
    19801 aatgctggtc gtgccgctgg gggttatcgg cgcgctgctg gcaacctgga tgcgcgggct
    19861 ggaaaacgac gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa
    19921 cgccatcctg atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga
    19981 agcgacgctc cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt
    20041 tatcttcggc gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc
    20101 ggtgggtact ggcgtaatgg gcgggatgat ttcagctact attctggcta tttacttcgt
    20161 gccgctgttc tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc
    20221 aataaaaaag gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct
    20281 cagcgggttc ttgctgttta tactttgtct cgggaattac ttacgaagca taacttcgat
    20341 aaagtctttc cagttcccca gttcacgttc aatcataaca acctctctta taattatggg
    20401 tattctacgg aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgactac
    20461 ctccggggga caaaccttat gtaaatacta tggtcctgcg gtgataattt gtatgtgata
    20521 cacagcaaca tttcgagata ttcatacggc atctaatact gatttaattc tggttaaaat
    20581 acagacagat a
//
LOCUS       DAZFKM010000085        20566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFKM010000085 DAZFKM010000000
VERSION     DAZFKM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHKAY_003235"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHKAY_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5701871.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1382..1541)
                     /locus_tag="ACHKAY_003236"
                     /pseudo
     CDS             complement(1382..1541)
                     /locus_tag="ACHKAY_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1744..2064)
                     /locus_tag="ACHKAY_003237"
                     /pseudo
     CDS             complement(1744..2064)
                     /locus_tag="ACHKAY_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(2486..3061)
                     /locus_tag="ACHKAY_003238"
     CDS             complement(2486..3061)
                     /locus_tag="ACHKAY_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5701872.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(3130..3708)
                     /gene="terD"
                     /locus_tag="ACHKAY_003239"
     CDS             complement(3130..3708)
                     /gene="terD"
                     /locus_tag="ACHKAY_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO5701873.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(3757..4797)
                     /gene="terC"
                     /locus_tag="ACHKAY_003240"
     CDS             complement(3757..4797)
                     /gene="terC"
                     /locus_tag="ACHKAY_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO5701874.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(4820..5275)
                     /locus_tag="ACHKAY_003241"
     CDS             complement(4820..5275)
                     /locus_tag="ACHKAY_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO5701875.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(5298..6455)
                     /locus_tag="ACHKAY_003242"
     CDS             complement(5298..6455)
                     /locus_tag="ACHKAY_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5701876.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(6455..7036)
                     /locus_tag="ACHKAY_003243"
     CDS             complement(6455..7036)
                     /locus_tag="ACHKAY_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO5701877.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            7359..8417
                     /locus_tag="ACHKAY_003244"
     CDS             7359..8417
                     /locus_tag="ACHKAY_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO5701878.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            8427..9569
                     /locus_tag="ACHKAY_003245"
     CDS             8427..9569
                     /locus_tag="ACHKAY_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO5701879.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            9562..10335
                     /locus_tag="ACHKAY_003246"
     CDS             9562..10335
                     /locus_tag="ACHKAY_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701880.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            10337..11416
                     /locus_tag="ACHKAY_003247"
     CDS             10337..11416
                     /locus_tag="ACHKAY_003247"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO5701881.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            11416..12372
                     /locus_tag="ACHKAY_003248"
     CDS             11416..12372
                     /locus_tag="ACHKAY_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO5701882.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            12383..13591
                     /locus_tag="ACHKAY_003249"
     CDS             12383..13591
                     /locus_tag="ACHKAY_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO5701883.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISRAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            13609..14076
                     /gene="terW"
                     /locus_tag="ACHKAY_003250"
     CDS             13609..14076
                     /gene="terW"
                     /locus_tag="ACHKAY_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO5701884.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            14337..14666
                     /locus_tag="ACHKAY_003251"
     CDS             14337..14666
                     /locus_tag="ACHKAY_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5701885.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            14653..15033
                     /gene="tnpB"
                     /locus_tag="ACHKAY_003252"
     CDS             14653..15033
                     /gene="tnpB"
                     /locus_tag="ACHKAY_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5701886.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            15076..15906
                     /locus_tag="ACHKAY_003253"
                     /pseudo
     CDS             15076..15906
                     /locus_tag="ACHKAY_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(15937..17550)
                     /locus_tag="ACHKAY_003254"
     CDS             complement(15937..17550)
                     /locus_tag="ACHKAY_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO5701887.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(17581..17931)
                     /gene="tnpB"
                     /locus_tag="ACHKAY_003255"
     CDS             complement(17581..17931)
                     /gene="tnpB"
                     /locus_tag="ACHKAY_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5701888.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(17928..18353)
                     /locus_tag="ACHKAY_003256"
     CDS             complement(17928..18353)
                     /locus_tag="ACHKAY_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5701889.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            18407..19158
                     /locus_tag="ACHKAY_003257"
                     /pseudo
     CDS             18407..19158
                     /locus_tag="ACHKAY_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         5068 a         5348 c         5250 g         4900 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaatgcca atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta
      901 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt
     1081 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     1201 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     1261 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     1321 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     1381 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     1441 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     1501 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     1561 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat
     1621 aagtcccttt gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca
     1681 ccacctcgcc acgaccaaat ccaaataagt caattgcacc ttctcaatcg ccattttgtc
     1741 agtaagcgta cagcctgaac tgatggtatc ttcaccatca atgacaacgg tgatcgcaat
     1801 tttactgacg ttcgccggaa cacgatccag tgctatctca atgctggcct gctgcgaacc
     1861 ggtaacgagc ctgactgccc cctcaggaga agacaaatta ttataaaaga taaagtcaga
     1921 atcgccactg acctttccct gagcattaag catgaacagg caggtatcgg gttcgccttt
     1981 aaagccggat ttggctgggt actgaagatt cagcctgatc gtagattgct gaaggggtat
     2041 gttttgtccg gattgtaaat tcatattaac tctcctgatt tttgattatt attaatgcgc
     2101 agcgtttata tatgttccct aggcttagtt ctggacgctg gatattcggt gaggcgtaaa
     2161 tatggtatga caccattttt cataacgctg aagtttctat acctgttgaa tttgaatttt
     2221 cattgaccgg gtatcttatt ttccagggcc cgttccttca taagtcgcaa aagtaacata
     2281 tatccgaagg gcatgctgtt gatatcagac acggaatact ggctttaacc agcaaccaca
     2341 gataaacccg gggcctgcat aaaaggttca tgccagaatt aacatagctc tgctttctgc
     2401 cccccccttt cccatattca cccgttgata gcggatcgat acccaaaaaa aaccccggca
     2461 ttgccggggt cagactcagc gtcttttaga cattgacgcc gtgctgggaa gcaagcgctg
     2521 acagaccacc ggcaaaaccc tggccaacag ctttaaactt ccactcagta ccatggcgat
     2581 acagttcacc gaagaccatt gcggtttcgg ttgaggcgtc ttcagacaga tcgaaacggg
     2641 caatttccgt cccgttgtcg ttgttgtaaa cgcgcatgaa gctgttgctc accatgccga
     2701 agttttgttt acgcgcttct gcatcataga tggtaacggc aaataccagt tttttgatgt
     2761 ctgctgagac tttggtcaga tcgattttga cctgctcatc gtcgccgtcg ccttcaccgg
     2821 tacggttgtc gccctggtgc tctactgcgc catcagggct ggttttatta ttgaagaaaa
     2881 tgaaatgggc atctgacagt actttaccgt cttcacctac tgcgaatacg gaagcgtcca
     2941 gatcaaaacc ctgaccatcg gttacacggg catcccagcc caggccaacc atagcgacat
     3001 tcatggttgg tgcttctttg gtcagagata cgttgccgcc ttttacgaga gaaactgcca
     3061 tttttagctc ctgcaaacag ttgaatgagg ctgaattaac agccccagat atgaaaagtt
     3121 acttttcgat caggacgcgt taatgccgta ctgagcacat acagatgcca gaccaccagc
     3181 ataaccctgg cctactgcgc ggaatttcca ctcaccattg tggcgataca gctcgccgaa
     3241 cagcatggca gtctcagtgg acgcatcttc ggtcagatcg tagcgagcga cttcagtctg
     3301 gttatcgtca ttaaccagac gaataaacgc accggatacc tgaccaaagc tctggcgacg
     3361 agcctgagca tcgtggatgg tcacaacgaa gatgatcttg tcaacttcag acgggacggc
     3421 gtccagttta attttcagcg attcatcatc accatcgccc tcaccggtgc ggttatcgcc
     3481 ggtgtgcgtt acggaaccgt cggatgacgt caggttgtta tagaagatga aatctgaatc
     3541 gccgcgcact ttgccgtttg aggccagcag gaatgctgaa gcatccaggt caaagtcctg
     3601 accgtctgtt gaacgcgcat cccagccaag gcccaccagg acatttttca ttgacggagc
     3661 tgctttactc agggagacgt tcccgccttt ggaaagagaa acactcataa aataacctct
     3721 tcgattagta attgttcagg ttaacactta aggggattaa ctcccctttt cctcagattc
     3781 aggtttgccc gggaacatga cgcttgcgag aatgcccagc gccagtacac ccagtacaac
     3841 atacaggctg gttgttgccg cgatgctgta accatgatgc cagatgtgat cggtcgcatt
     3901 caggccgagt tttgccgcga tgaagaacag cagcacgata acggccttct ccagatgaac
     3961 caggtactgt ttcagtgcct caaggacaaa atacagagta cgcaggccca ggatagcaaa
     4021 catcatggca ctatagacga taagcggttc acgactgacg gcaatgattg ccggtaccga
     4081 gtcaaacgcg aacattacgt ccgagagttc aaccacagcc acacacagga acagcggggt
     4141 cgcatacagc gccgcttttt taccacggcc aatggtgaca tcgctgtttt ctggtttcgc
     4201 cagttcagca tccacttcct tctggttaag taggaaggca tgccctctga gcttcggcca
     4261 gatagggaag aagcgtttaa ccatacggta agccagatgc tgggagtaat cctcaatttc
     4321 atcatcgtca tcaccgcttt taagcatcat gaccgccgtc caggcaacga taatagcgaa
     4381 gacaacttca acgtacggcc ccagactcag caggctcgta ccgatggcga caaagatgcc
     4441 cctgaagacg atggcaccaa tgatccccca gtagagaaca cggtggcgat aacgatccgg
     4501 aacggcgaac caggagaaga ttgccatcat gacgaacagg ttatcgaccg acagcacttt
     4561 ctccagcgca taacccgtga caaacagact ggcaacctca gcaccgtggt ggatatagag
     4621 gaaaccggca aatgccatcg caaccacaac ccagaatacg gaccagagcg cagcactctt
     4681 cagcgagatt ggcttgtcat cacggtgcat aaacaggtca ataaagatgg caccgactga
     4741 aagcgcaata aaaacaatga ccgtttcagt cgggaagccg atgtgtgtgg aaaccatatt
     4801 ttacccttag ggatgtggat cagaggccaa attcagccgg gtcgaagccc aacgcgatag
     4861 cgatctcgcg ggaggccagt ttctcgtcct gatcgaagtt accgtcactt ttcgcaacgg
     4921 caataccaac acgtaaggcc agctgagcgg cctcaggctg atctttcagc gccaggatgt
     4981 atttcatggt ttcgcccttg ccaatttcaa catcgaaatc gaagcttgaa accagtttat
     5041 tgaagaactc aatcacctca ttggtatcga agaccttcag ctcttctgaa gagcgcagaa
     5101 agcccatcat tttctgcttt tcttccgaac ttacgccgtc actcgatacg gcaatacggg
     5161 cacatacagc aacggtgccc tgcatgaatt ttttgttttt gaaacggcca acctggcggg
     5221 tcagttcgtc acggcctgag ttaatggcac ctttaacttt gtcgaaaaag ctcatacctg
     5281 tttcctttgt tgctgtgtta tttagaaccg gcgctccagc gaaatcccca gccaaatgcc
     5341 cggtccattt cgtcctggcc tttgaagtac tggttaattc gctcgacttt aattgcgccg
     5401 ttttcgttta cgagtctggc aatggcgcac aatgtgcgac ggttctcacc ttcggtcagg
     5461 cgggtctcaa tgggcggttg atccggcaca tgaatggtga ctacaccatc agtcttgtcc
     5521 cagctgggaa caccttcata aataaaggcg taaatcagca cttcgcggat atgcttccat
     5581 tcacgtccat tgatatgcag ccactcgccg tctgatacat cacccgtccg gtcatcgccc
     5641 ttgagctgaa catacggttc atcgcgataa tcaccgaatg cattaccgag cgcctgaatg
     5701 accgatttat acccgtcctg aagctcaaca aaggccccca gatccaggtc gattcctttg
     5761 gagccgaaca tacctgcaaa gcctgatttg ctactgcccc ggtgccagtt caggttgatc
     5821 cggatttcac cgaagttatc ccgtttggtc aggctgattg caggcttctc ttttgtcagc
     5881 gataccttgc tcaggctgat ttttgtctca actggcggag gggttacaac cggaggagtt
     5941 ggggctgcgg gagccggttc gtcggcaata tttacgccga aatgctctgc cagcggttta
     6001 agtccaccgt tgaacccctg cgcaacgaag cggaatttcc agtcattatt acgacggtaa
     6061 aattccccca gaattaaggc tgcttcctga cggccgctca gttcgacact gccgctaacc
     6121 aggccggtag ccccttgctc cacgtcgatg gaaagattac gaagaccgga caccgtctgc
     6181 ccaccgtcac aggtcacggt gaacgcaatt ttctggacat caggtttcag ccggttgagc
     6241 gcgacagtaa aggttgagta ctggccttca ctgaccaggc tgacggtacc atcatcgtta
     6301 cgtggctgac cataaaacac catgtccgca tcaccctgca ccttcccgtc ggcatacagg
     6361 cgaaacgccg aagcatccac ctggccgccc gaggtgatcc ggacccgcaa ctcctgggct
     6421 ggaacggggg cattgccccc cggcgtcagg ttcattagcg aataaccgct gaaacgatat
     6481 cggagtgcat atcgtcaatt gtgcggccgc gacaggcatg gccaagggcg gtaaagtccc
     6541 agttgccgtt attacggcgc agcgaagaaa taacgatccc ggtatgtgag ccctgctcag
     6601 tcagcttgta gcgagccagc tctttgccgg tttgatcgac aacgcggcag aaagcgtttt
     6661 cgacgtcatt gaacgactga ccacggaagc tgtttaccgt aaacgccagg tattcaacgt
     6721 ttgcaggcag gcgggagagg ttgaccttaa tcacttcatc gtcaccgtca ccctcaccgg
     6781 tcaggttatc gccactgtgc acaacagcgc cgcaggtgga tttcagtttg cggaaccaga
     6841 tggtgtcaat tgttttacct gtgctgtcca ttaatacaca gccagcatcg agatcgattg
     6901 agtcgttgcc tccaaacaat cctccaagaa gacctttttt cttaaccgga tcccaaccga
     6961 gaccgaaatg aagctggctt aatgcagatg attctttgct gagtgatacc gtctggttct
     7021 taactaatga aaccatgttt tattccttaa aatggtaatg accgagacaa tcaaaggtat
     7081 atttagtaac tgacgaagaa aatcatatca tgatgaagtt ctctgacaag ggaaattttt
     7141 gtggttttct gtaaaaacac aaaaagaaat ccttttcagg gtgttagttt ttctattttt
     7201 aatttaaata tcaatgtgtt ataaggaatc caaaaaacac tgacgcactc cataaccaca
     7261 ggagttatta atcatattat gattgacatg tagcttagac actcatagac tcacttctca
     7321 atcataaatt tgcatggcgt tccggataat taaaaaaaat gaacaagaaa atttggttta
     7381 tggaaggttt atcctcccag cgagatatta ttcagggggt aaaatcattt gcacaaaaaa
     7441 ataattttgc cattaccgtt tttgcctccc accgtaacga aagaaatgaa atcctttccg
     7501 ttgccgatta ttctttgact gaacctgaag atcctcaaaa acgtcttcag tttatccagg
     7561 aaaccattca gacctacggc atccaccata ttcatactgg ccgtaacagc cagtggtttg
     7621 aagaacaccg ttcagccatt gaaccgaccg gtgccaccct tactaccggt gcaacgggcg
     7681 tcgactggtt aactctggct gacgaaaaag ttacttttgc tcagtttatg gagcaaaagg
     7741 gtctcccggt cgtaccatcc tggcgggtga atacgctggc agaattaaag acacacctcg
     7801 cggccccgcc gttcactgac agcccggtat gcgtgaagcc ggtgacgggt atctatggca
     7861 tgggattctg gcgctttgat gacagtgctt cgcctatggc cgtctttaat catcccgaac
     7921 atcgtctggt cagtccgcaa cagtatattg cagcagcatc agctgctgag tcgtttaaac
     7981 cccttgtttt gatgccgtac ctgccaggcc cggaattttc cgtcgatatc ctcgcggata
     8041 agggcgaaat actcgcagcc gtgggacgcc gtaaggaagg ggctatccag tatctggtaa
     8101 acgaaggaag cgcctgggaa ctggcgtgtg actgcgcccg tgttatgaag gccgacgggc
     8161 tggtgaatgt tcagacgcga aacgatgtga atggcaaccc ggtgctgctt gaaaccaaca
     8221 tgcgtccgtc agggggggtg ggttataccc ttcacagcgg tgtgaacctt cctgggttat
     8281 ttgctgcctt taagctcggt ctgatgtctg aagatatggt acgccagagc gctaaaaaca
     8341 ccttttctcc ggttgcggtg agatccatta cggatgtaat tgcatacccg gaatcactct
     8401 ctaaccttct gaattaaggc gtatttatgt ctgatatcct cgaagatatg atttatcgcc
     8461 gtaccctctc atgcggtacg attcaggtaa cccgtgacca gggtgaagtt tcgctcgatg
     8521 acctgtttga catcgctgaa agacgtaacc cgaaacgcgc ctttctgttt gtcagcaaag
     8581 tactcggcag gcacattccc gtgccaccct cagtcatgcg acaggcctac agacagcttg
     8641 ccagccagtt cccctcgaca ctgacaggac ccgtactgtt tatcggtatg gctgaaaccg
     8701 ccgttgggct tggggccggt gtatttgatg aagtgcgcca ccagcatcgt gaatctgtct
     8761 atctgacctc tacccgtcac ccggttgatg gcacgttgct ttgcgagttc aaggaagaac
     8821 acagccacgc caccgatcat ctgatctatc tgccagatga tgaagagaaa agacgtcgtg
     8881 ttaccaacgc acgaacgctg gttttgattg atgacgaggc aaccaccggt aataccttta
     8941 ttaacctgct ttctgccctg cgtaataccg gcaagcttca acatattgaa caggttatcg
     9001 ccgtaacgct gaccgactgg agtggcaatg ccctgtcgga gcgcaccccc ttgcccgtca
     9061 cttcggtttc gcttgtaagc ggtcagtggg gatggacccc attacctgat gcacctgtcc
     9121 cggacatgcc gaaagtcaac gtaacttcac gaggagaatg ggacatccag gggaaacagt
     9181 catggggccg actggggatg ctcgcacctg cggccgatct cggccatgag gtctccgtcc
     9241 acaaggggga acgtattctg gttctcggga ccggggaatt cgtctgggag ccgttcctgc
     9301 ttgctgaacg gctcgaagct gccggagcac aggcatttta tggatcgacc acccgctccc
     9361 ctgtcgccgt tggttatgcc attgagtccg ccatttcctt tacggataac tacgggctgg
     9421 gcatccccaa ttttgtctat aacgtcgccc accagcagtt tgaccgcatt cttgtgtgta
     9481 ctgagacacc cgcagaaagt attgacacgc agcttcttaa agcgctggct gaggttgcgc
     9541 ccgtcgtgga gattgttacc tatgaataaa cccgttgttc tgagcgacct tgacgacacg
     9601 ctctttcaga cccgtcgtaa aatggtggac gagctggccc tggagccatt ccgtaccggt
     9661 gccgttgatc gcaccctgaa tccacgaagc tttatgacgg aagaacagtc catgttggtg
     9721 gactggctcc tggagcaggc tgaactcata cccgttaccg cacggggaac tgaagaaatc
     9781 agccgcgtac ggatcccttt ccactcctgg gcaatcacaa cgcacggggc cgtcattctc
     9841 acgcctgaag gcaaacccga cgaggagtgg aaggcccata tgctcggcca gctggctccc
     9901 taccaggaaa agctgacatc gatgcagcgt ctgatcactg aaatgatgga cgcaaaagga
     9961 atcaatgcct gggcaaggct gaacttcgaa tacggtgaaa cggcggttta tatggtgatg
    10021 aaacaccgcg acagcacccg ccttgacgag ctcaatgcca ttgcagatga gatagaaacg
    10081 gtgtttccga ccgagggctt ctacatccac cgcaacagta ataacgtggc ctggcttccc
    10141 accccggttg agaaagggct ggcagtcagc tggctgcttg aaaaacttcg ggctgaacga
    10201 ggagttttcc ccgtaattgg tttgggcgac agcctgagcg atcatcgttt tatgaaactg
    10261 tgcagctggt ttggcatccc gcgtcagagt cagtttgcag atgccatttc acagcgaatt
    10321 tttggagaaa attaaaatgc agccacatga aacatttacc ggctcatacc agcccggtga
    10381 cgtggaattt ctgctaaagc cggttgtcat tgagatgacg ccggttgaga aaaaagaaga
    10441 actgattcag tccgggaaga aacattattc ggacatgctc agccaggagc cagcgcctac
    10501 acaatggcat cttgatttgt ttcaccgggc gctggatcgg ggcgcagaga ggcttgcaaa
    10561 agaagtcaca cagctcgcta ttgctctggc cgaacgcttc ggtgatgagc ccattgtact
    10621 ggccagtctt gtcagggctg gcgttccgct cggcgttatg ctgcaccagg ccctgcgtga
    10681 catggggaaa acctcatggc attacggtat cagcattatc cgggatcgtg gaattgacgg
    10741 tgcagccctc gacgtcattg aagagcggca tggtaccagc ggtattgtct ttgttgacgg
    10801 atggacaggt aaaggcgcaa ttaccggaga gcttgtacgc gcactgaaag atcgcccggg
    10861 ctatcctgag cagccgcgac tggttgtcct ggccgatccc tgtggctgct cctggcttgc
    10921 agccagtgat gatgactggc tcattccttt tggcatcatg ggggcgccgg tatcaggcct
    10981 gatctcccgg tcagtatggt cctctgaagg attacacggg tgcatggttt gcgagcatct
    11041 cagtgaattc gaatgcagcc ggatgcttgc cgataccgtc gctcatttcc gtaagaagtt
    11101 aacaccgtca tccctcgctc ccctgagctg gaatacggaa tcagcccggg tcttatggca
    11161 gacaagtcgc gacgttatcg cgttcctggc cgatgaattt aaagtggaca gcgtcaatcg
    11221 tattaaaccc ggtattgctg aagcaacccg ggctgtatta cgtcgggtac cggaccatgt
    11281 atttgtgcgt tctattgacg acccggacgt tgccttgctt gtagggcttg ctcgtgaaaa
    11341 gggaatagtt gttacagaaa tggggggaac cctcggccag tatcgggctg taaccattat
    11401 caagaaggta ctctgatgaa aaatcgtctt tctccctgga atctgggagc cacgctatac
    11461 atgcctgcaa cacgggaaga tattgctgat gccgtcctgc acgggaaaat cccgggcttg
    11521 cgctctctgg tgatttgcct ggaggatgct gtcagtgaag cagacatccc cgtcgccctc
    11581 aaaaatctgg agcacctgct gcacgagctc agtaacagca tgagtagcct gggtaaaaat
    11641 gactggcctc tggtgttcat tcgcccccgg catgccgaaa tgggcagatg gttaaaagcg
    11701 cattatgatc tttccgctgt cgacgggttt gtgctgccta aatttaccct cagttcgtta
    11761 gctgaatggt gggatatcat ggtcgggact cacctgtgca tgatgccaac gctggaaaca
    11821 gaagatgtct ttgacgtggt tcagatgcgc gagctggcta cccgtctgga ggaacatccc
    11881 tgccacgacc gcattattgc gcttcgaatc ggcggcaacg atcttatgaa cgttgtgtcg
    11941 cttcgccgtc cccgggacct gacgctgtat gacagcccga tgggctacgt catcaaaatg
    12001 ctcgtttcgg tcttcggccc gcgtgatttt gccctgaccg cgcctgtatg tgagcatatt
    12061 gacgatcatg ccgttatggc cagagagctg gctcttgata tggcacacgg gcttgttggg
    12121 aaaacggcca ttcacccggg tcagatagag gtcattcaaa acgcgctgat ggtcactcag
    12181 ggtgagcatt ctgacgctct gcggatcctg aactctaccc aggccgtgtt taagtcgcag
    12241 ggagcaatgt gtgaacccgc cacacatcgc cgctgggcgg ctggcattct ggacagggct
    12301 cgtttttatg ggttacagaa cgagcaaagc gctgatggaa tcagattact taccgtgacc
    12361 cagcatcatt aaggaaatca taatggggtt caggttcaga aagtcgatca acattattcc
    12421 tggcgttcgc ctcaacctga gtaacggtgc accgagcctg agtgtcgggc cgagaggtgc
    12481 ttccgtttct tttggtagcc gggggaccta tgccaatctg ggcttgcccg gtaccgggct
    12541 gagttaccgt acccggcttg accgggccgc gcgttccaga ggtgaaaacc ggacggcaac
    12601 cgacccgggg ctcagacagg cgcttgagca gaaagccgct gaactcatgt cagcggtaac
    12661 cgcaatccgt aatattcacg agctgacgcc ggatccaaaa acaggcatca gcagggcaga
    12721 gctggaagca gtatacctgc ataacagaac gtcgcctttt caggttccgg caccggtgcg
    12781 tccagaaaag ccagactacc ttgtattgcc ggaaaagcct gccgagagcg aaggcattag
    12841 ttttttgggt aaatggtttg aatcggaatc agctaaagct gagcgccacg ccgaaaatct
    12901 tcgccggtgg cagcaggagc tgattgatgt ggagcgtgag aatacccttc gacagcaccg
    12961 gtaccagcaa caacggacgg cctgggccga acagtatgca aactggaagt ttgaagctga
    13021 agaacatgaa aaacggctcg ccacggctca ggcagatgcc cggcagcagt tccggacaga
    13081 cgccgcgttt ttcgaatcat acctggcggg tgtgctggca gaaactgaat ggccgcgtga
    13141 aacgcttgtt gcatttgaag taaagccgga gctatcagca gtcctgctgg acgttgattt
    13201 agctgagatt gaagatttcc ctgataagat ttacggcgtt aatgcccggg gaacggagct
    13261 gacggaaaaa gccatgacgc aaaaagccgt acgcgaaaac tatgcccacc acgtccatgg
    13321 ctgcttgttc cgcctggtcg gtatcgtttt acatacgcta cctttcgaca acgtgattgt
    13381 gtcaggcttt acgcaacggg tcagtaagcg gaccggctat ctggaggatg agtatatcct
    13441 gtcctgcaaa tgcactcgca gccagatgtc gtcagtaaat tttgcaggca taaaacacat
    13501 tgatccggtt gaagcgttag gcgatcaacc ggttattcga aagatgagca gtaccttcat
    13561 ttttcagcct attgaaccac taacccttta agcaggctaa atcttacgat gcaattaaat
    13621 accagacagg cccggatttt taagcttgcc aacttactgg gcaccggcaa gcccgtttcc
    13681 gctgccgaca ttataaccag tctggaatgc tctgagccga cattaacccg ggccctcaaa
    13741 gagctacgcg agtcatattc tgcggagatc aaatacagca aggcaggtca ttcctatcat
    13801 ctcgttaatc cgggccagtt ggataaaaag accctccgcc ggatgaatga agcgctcgca
    13861 cagaatgctg aactgaaaac cggtgagtcc acagggaagg tcgtactcga taaggataaa
    13921 aaaacagccg tgtcattatc gctacgcatg cggattctaa gaaaaattga ccgtctggct
    13981 gcgctgagtg actcgacccg aagtgaagcg gtagagaagc tggctctgca ctccgttgat
    14041 gaactgataa aagaatacag cgctaaaaag tcctgacgct aaatctcaga ccggggtgaa
    14101 aactccggtt ttgtcatatt tcacctctgt attggcgtga tgcaaaatgg catctgaatc
    14161 gatcatggtt tggcatccat ttccgatcac agtttggcat cacaatcgat cacgatttgg
    14221 catgcttccg ctcattgatt ggcatcctgg tgtcgttacg gggaagtcat cttcacaggg
    14281 atcttcagtc agttagcatt accgacatcc attcaccaac ttaccggaga taatcaatgt
    14341 caaaaccccg atggacgctt gatcagaaaa aacatcacgt tgccgcatgg cgcgccagcg
    14401 gccttacccg tgaacagtac tgtgaacttt acgacattcc gtttaaatcc ctgcgccagt
    14461 ggccgcagga tgtcgcaaaa gccgaaaaac gagccagagc accagaaatt attcctgttt
    14521 cagtatctgg ctcttccgga atgactgatg gcagaccatt gtcggatgag cctgtcactc
    14581 tcttcctgcc cggtggtatc cggatgtgct gccagccttc tcagttaacc gatgttttcc
    14641 gggctcttcg ccatgctgac gcctgataac gtattcctgg tcgtaaaacc cgtcgatatg
    14701 cgccggggta tcgacacact gacacagtac gtccagaacg aacttaacgc tgcatggcat
    14761 gacggtgctg cttttgtctt caccaataaa gtccgctccc gcataaaagt tctccggtgg
    14821 gacaaacatg gcgtatggct gtgtacccga cgactgcacc ggggaagctt ccgctggccc
    14881 cggaaaggtg atgccacatg gcatctcaca caggatgagt ttcactggct ggtctttggg
    14941 gttgactggc aacaggtgaa agggcatgac ctggcaaaat gggtatacca ggaagaaccc
    15001 gaagatgctc agagcaacgc aataactcaa tgaaatataa tgaatatcca tgcgtgatgt
    15061 catataatcc accgcatgga tatctcgctt ctcagtacca ccagtgaccc tgaacagctt
    15121 cgcgctctgg caattgccat ggtgcaaaaa gtcatggctg agaatgcaga acttcagaac
    15181 cgtatccgca tcctggaaga gcagatgaag ctcgcacgcc agcagcgttt cggtaaaaaa
    15241 tgcgaatcgc tggccgggat gcagcgttcc ctgttcgaag aggatgttga cgcggatatc
    15301 gcagaaatct cggcgcatct ggacaaactg cttcctcaga ccggggacga agaaaaaaca
    15361 acaacacgtc ctgttcgtaa gcccttgcct tcacctcttc cccgtgctga aaaggttatc
    15421 ccgccagctg aggaacgttg tcctgactgt gatgcccccc tgcacttcat tcgcgatgaa
    15481 gtcagcgaga agctggaata tattccggca caggtcgtgg tcaaccgcta tatccgcccg
    15541 cagtacagct gtccctgctg tgagaaagtg ttcagcggaa aaatgccggc acatatcctt
    15601 ccgaaaagtg ctgttgagcc atcggtcatc gcacaggttg tcatcagcaa atataccgac
    15661 catctgcccc tgtatcgcca gcagcacatt ttctcccgaa tgggggtgga gctccccgtc
    15721 agtacaatgg cggatatggt cggtgttgct ggcgctgcac tggccccgct ggcaaaattg
    15781 ttacgccacg agcttctgac acgggatgtt attcatgcag atgaaacgtc cctgaggctc
    15841 cttgataccc ggaaaggcgg taaatcctgc tccggctggc tgtgggctta cgtcagcgga
    15901 gaaagagtaa gcgtcaacgg agcaccgtat tgacgcttat ttattggtga gtactacgtt
    15961 ccatggcagg agttcatcaa ctcggttgga aggccattct ggcagtacgc tcaggatatg
    16021 gcgcagatac gcttccggat cgataccgtt cagacggcag gtgccgatca gcccgtacaa
    16081 cagtgctcca cgctcgccgc cgtgatcgct gccaaagaac ataaagtttt tctttccgag
    16141 acagactgca cgaagcgctc tttccgcagc attattatcc gcctccgcca gaccgtcatc
    16201 actgtaataa cagagggcat cccactgatt cagtacatag ctgcacgctt cgcccagtct
    16261 ggattttttc gacagcgtac cattcttctc caccatccat tcatgcagtg acgtcagtaa
    16321 cactttgctt cgctgctgcc tgacggcaag acgctctgac tccggtaatc cccgtatttc
    16381 atcctcgatg gcgtacagtt cactgattcg cttcagggct tcttctgccg tcgcactttt
    16441 gctgctgatg tatacatcgt ggatttttcg ccgggcatgg gcccagcacg caacttctgt
    16501 cagtgcacca ccttcacgtt ctgcactgaa cagccgatcg taaccgctga acgcatccgc
    16561 ctgcaggata ccccggaagg gacgaaggtg ttgtaccgga tgttttccct gcctgtctgg
    16621 tgagtaggcg aaccagaccg ccggtggctc tgatgagccc gcattccggt catcccggac
    16681 atacgtccag atgcgtcctg tttttgcctt ttttctgccc ggtgccagca cttttactgg
    16741 tgtgtcgtca gtgtgaacct tgcgggtgtt catcacgtaa cggtacaggg tatcattcag
    16801 cagagtcatt aactgacaac atgcgtcaac ccagttggag agtaatgccc ggctcagttc
    16861 gatgccctgg cgggcaaaga tttcactctg acgatacagt ggcaggtgtt cgcagtattt
    16921 tcccgttaac acgcgggcaa gtaatccgga gcccgcgatg ccgcgctcta tcgggcggga
    16981 cggcgctggc gcttcaacta tacagtcaca ttttgtacag gcttttttta cccgaacagt
    17041 gcggatcact ttcagggcgc tactcaccag ttccagctgc tcagcactga cttcccccag
    17101 ataatccagc tcactgccac actccgggca acaactttct tcaggctcca gacggtgtat
    17161 ttcacgggga agatgtgccg gtaacggacg acgatgacgt gactgtcgca actggcgggg
    17221 aacctgcggg tcatcctcac gcccactgta acgatcgctt tcctgttcgc gttgtttcag
    17281 ttgggcctca gcctgttcaa cctcacgctg cagtttttca gaacgggtac cgaacaacat
    17341 ccggcgcagt ttttctatct gggccctcag atgttctatt tcccgctcct cctcttcgat
    17401 cttttcctcg gcacgtgcca gtgcagagcg caggaaggcc tccgtctctt caaccagact
    17461 cagttgctga tctttctgac ggagggcttc agcctgctca gagagcagcc tttccagctc
    17521 agtgatacga atgaggtatt tccgactcat gaccgttttt ataatccggc catgacattt
    17581 ttacaacatt gtcagtgcat taaggcggga tgttttgggc tgacgccagt ccagtttatc
    17641 gaggagcatt gccagctgcg agcgggtaat ggatacctta ccgtcacgca ccgcaggcca
    17701 gataaactgg ccttcctcca gacgtttggt gaacaggcac agaccatcag catcagccca
    17761 cagaatttta atcgtgtcac cccgtcggcc gcgaaagata aacaggtgac cggagaaggg
    17821 gttctcatcc agcacatgtt gtacctgttc tcccagaccg ttgaaggatt tacgcatatc
    17881 agtaacgccg gcaaccagcc agattcgagt gcctgatggg agcgagatca tcgtcccctc
    17941 ccggtcagtt cacggatcaa caccgtgagc agctctggtg aaggattttc cagcgtcatg
    18001 ttaccgtggc ggaactcaac tttacaggaa ctggcactga ctgtgctttg tgaaggagtg
    18061 gataaaagcg gagtaagagc cgccataggc tctttctgct catcaggcgt tatctcaaca
    18121 ggtaataatt caacgccagc gccagaagag gttgttaccg gaagacgccg cgatatacgc
    18181 ctttcgttct gccagagcct gagccatttg aacaggaggt tatcattgat atcgtgttcc
    18241 cgggcaatac gggcaacaga ggctcctggt tgtgaagcca gtttaaccat ttgaagttta
    18301 aactcatttg aaaatgatct gcggggttct gaggataata ttttctgttc cataacaggt
    18361 gtccactagt tgaaaaggtg ggcacctacg ttaccaatac tggcttaatg gctacatacg
    18421 gcggtcagtt tacgcttacg agaaagaagc ggtccgccgg tcgtctgttt tgactcgcag
    18481 accggacggg ccctcagata tcctgaggcc tggctgcagg gctggtgtgg cggtacgctg
    18541 gtgagtgacg gatacagcgt gtacaaatct ctggcggata atgaccccgg tattacatct
    18601 gcctgttgct ggagccacgc aaggcgcggt ttcgcaaatc tgtataaggc cagcagggag
    18661 ccccgtgcag ccatggcgct gagaaaaatc gcgggtcttt atcgcattga gaagttcatc
    18721 cgtgaacgtc ctgtggaaaa aatacggcaa tggcgacaac ggtattcccg gccgatagtg
    18781 aatgacctgt ttgcctggcc tgaagagcag gagccgtgct gtccgcccga cggaccacta
    18841 aacaaagcga ttaattatat cctgaaccgg cgcgatgaac tgagctgctt cctgagtgac
    18901 ggagccgtgc ccctggacaa caatatctgc gagcgtgcta tccgcccggt ggtcatgggg
    18961 cggaaagcat ggttattcgc cggttcactg atggccggga accgcgcggc acagataatg
    19021 agccttctgg aaacggcaaa acgtaatggt ctggagccgc atgcctggct gacagacgtt
    19081 ctgacgcgtc taccggagtg gccggaagac cgactggaag agttgctgcc ccttgaggga
    19141 tttaccttct ccgggtgagt gatacctgcc atcaggtgtt cgtgcagcgg gccataaccc
    19201 gcagtcggga gttgaacgcc tgacgacagg aaatgagcca gagcagcgac cacgcgggct
    19261 gacagtgagt cagaacctga atcagcccgg agccattccc ggggctgtac ttcatcacct
    19321 gccatcagta cccgtgtcag tacttcgcga cctgacgcaa ttacccgact cagttcttca
    19381 gaacccgcct ccgataccag cctggcggtc gccccggcgc aacagaataa atcaaccatc
    19441 gtcagactgc gatcattcgt atgcatcgct gatgatgcgc ccgaaaaacg cgtcaatatg
    19501 agttcgccgg aatattactg acgacacggt ttgtagttac gttccttcgg tgagtgagta
    19561 cctgtgcgtc agggtaacgc aatcagttca ggtttagcgg ggtgtcaagt cggtttggct
    19621 aaccgaccgc agaattaact gtacctttaa aggtcgcccc cgatatatct gcctgtcaga
    19681 catatcgccg attcggtatc tcactacata aaactcctta aaaaacaatg cgattctgtt
    19741 ggtacgaagc tttcagtaag tcagtccgga gaagcgccag tacgatacag aatgaggttg
    19801 aaaaaaacgt tcacgtctgg tcacttcttc aaatgttaaa aaacatctcg taaagagcca
    19861 gtttatgcgg gttcgttgag tagacgtgcg agcgtcactt tgtcagcttt aacgctgacg
    19921 aaagtgacgc ccgcacagct tcaaactcca catgcacacg caagcgacca aatttcaaat
    19981 ggtcttatgc cgtgcgcaca gtcataagaa gcgcatgcgg aaagtcagaa aatatcttaa
    20041 gacgcagatt ttaaaggcgt ttttatgcca gtctgccggg aatacacagt agtccggttg
    20101 tccggaagca ccacggtaat ccgaaggaaa aatcaacgta cctcaatcac cgtagtgata
    20161 tccgttccca gaggctaaac ggatgtacca ccataaaggc atcctctttc aggctacgag
    20221 gatattgcaa tcacccgaag gcgtttctct cagattgctg aaacattggc tggcagtcac
    20281 ccgaaggcgt ccctctcaga ctgctggaac attggctggt cgcaaaagcg acagttaatg
    20341 caaatacagg ttaacaaaaa gggaaatctg ccgtcaaccc aaccatatga acatgttgat
    20401 ttcattatga atgtgatgtt ctaatataaa ttaatcagta acttcataaa aagaatataa
    20461 gaaacagata atgcatgtat ttacacacat atcagcacaa tgatagatta ccaccccaga
    20521 aacacgataa aggtataatt atggaatttt ccgttatttt tttctg
//
LOCUS       DAZFKM010000086        20523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFKM010000086 DAZFKM010000000
VERSION     DAZFKM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1113
                     /locus_tag="ACHKAY_003258"
     CDS             <1..1113
                     /locus_tag="ACHKAY_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5701890.1"
                     /translation="NMQRLPGVQADMEIVPGEQPGESDILITRKQDKYWRLGFSLDDS
                     GTRSTGRYLGGITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQF
                     SVTASDYDYHQTVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRN
                     YINDTEIQVQRRQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWFGAQPAPEEIFGEA
                     TALSKILQMNASFNLPFTLAGQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDG
                     ERTLNASNGWYIRNDLAWRTPLPEQELYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRG
                     QLFSTDYDVFIGRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            1123..1650
                     /locus_tag="ACHKAY_003259"
     CDS             1123..1650
                     /locus_tag="ACHKAY_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO5701891.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARFLTQPAILMPQSDWNSGDR
                     MWVCDWIAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            1666..10983
                     /locus_tag="ACHKAY_003260"
                     /pseudo
     CDS             1666..10983
                     /locus_tag="ACHKAY_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538208.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            10980..11465
                     /locus_tag="ACHKAY_003261"
     CDS             10980..11465
                     /locus_tag="ACHKAY_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701892.1"
                     /translation="MINVNSIAKDIEGLESYLTNGYVEANSFNDPEDDALECLSNLLV
                     KDSEGGLSFCKKILNSNNIDGVFIKGSALNFLLLSEQWSYAFEYLTSNADNITLAELE
                     KALFYFYCAKNETDPYPVPEGLFKKLMKRYEELKNDPDAKFYHLHETYNDFSKAYPLN
                     N"
     gene            11725..12366
                     /locus_tag="ACHKAY_003262"
     CDS             11725..12366
                     /locus_tag="ACHKAY_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFO5701893.1"
                     /translation="MLQTEKAREHGADVLSCSDNPSGEACKRGQAENKAYAAALATGS
                     VALLPGSAQAMWLLGVGTNAGMQYADSGEINPVNSVAAGWINVITMGQGWKGTIAWNA
                     AGGALTNAINGDDPLTGAITNGAGAGFGYGVGNYVVKPAANTIGKWITGGWNPKFDPT
                     LLKYTEVKGQLGLSKEMVPSKIPGAVGNAGGSLSSEFGSSIIQKKKDAMEAGK"
     gene            12654..13040
                     /locus_tag="ACHKAY_003263"
     CDS             12654..13040
                     /locus_tag="ACHKAY_003263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701894.1"
                     /translation="MTVCRKAGGDCGDIQNKYAVLNAESRHQLHLDVAADPLTALSGE
                     DKWNIEGGLSAAGRLGWLYGSLTNQEVKDYVAEGNSYDLNYLNSNTSKGDRALAFFGE
                     PENYWGTVAGAGSLLISSAHADGKIC"
     gene            13299..13856
                     /locus_tag="ACHKAY_003264"
     CDS             13299..13856
                     /locus_tag="ACHKAY_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5701895.1"
                     /translation="MKTLSVISAIILSVASGSVLADNHSFSVGYAQSKIQNFKNIRGV
                     NLQYRYEWDSPVSIMGSFSWMKGDGNQEYHSYGDSIKNQVDAKYYSLLTGPVWRINDY
                     MSLYAAGGVAHVKADGKTAWTNVDGYFARENISKKSTSFAYAAGVMFNPASNITINAG
                     YEGTRAKLGDNYSINGFNIGVGYCF"
     gene            complement(13892..14637)
                     /locus_tag="ACHKAY_003265"
                     /pseudo
     CDS             complement(13892..14637)
                     /locus_tag="ACHKAY_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15275..17749)
                     /locus_tag="ACHKAY_003266"
     CDS             complement(15275..17749)
                     /locus_tag="ACHKAY_003266"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5701896.1"
                     /translation="MSLDFDKLNAGIGAKQTDPRKIFTTLKRDARFKRPLDEQADVLD
                     VWYARRQAQDLTLKMNTGGGKTVVGLLCLQSALNENVKPAVYITPDSFLVNQVLDEAK
                     ALGIPATENEKDPDFLAGRAILVANVWKLFNARSVFGVGQKKIPIGCVVVDDAHACLS
                     VVHDQFSIKADVGTPVYDDLLNLFKNDLDKQSPTGLLDIQALDPSIVMEVPYWAWQSK
                     QKEVIALLHAQRNSAELEWSWPLLRDCIPLCTCLFGGGKLEIAPYFIPIDRIPSFATA
                     QRRIYMTATLADDTILVSHFGALADEVVKPIRPKGGGDIGDRMILAPQEINPDFKMEE
                     IRELVSDIAKDLNVVVIVPSKERAKFWKDSANQILNKDNIQAGIQRLKDGFVGLTVLI
                     NKYDGIDLPGKACELLVIDGLPEVYGLSERREMLLLDGTKRQLVRQVQRIEQGMGRGV
                     RSSDDHCVVLLLGGRLTQRLHQPEAETMFSSATRAQIELGKSVAEQLRGQSLEKLQEI
                     MGLCLTQDDNWVQAGRNAVVNAPPVEGGRIDDNQVLLREAFDAARVSRYDTAKNKAQT
                     AVNAASERQIKGYLKQQLAAYTNFTDPSQAQELQLAALSDNYRLLKPLAGTTYHRLSA
                     PASGQAAAAVQFMTRFLEGNDLIIWVNGLLDDLQWGEEGSKRFEAAIKELGIFLGFGS
                     ERPEDLVGRGPDNLWALGNSRYFVIECKSGAVLAERISKHDTNQLNGSIVWFDEKYGH
                     TCTRTPILVHPKTIFEHAASPHSDIRIVNEQGLNRMRDAIQAYSISLASNGGYADSQI
                     VHRQLKHHKLSAEDIEDLCTVAQGAK"
     gene            17913..18338
                     /locus_tag="ACHKAY_003267"
                     /pseudo
     CDS             17913..18338
                     /locus_tag="ACHKAY_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18435..18737)
                     /locus_tag="ACHKAY_003268"
                     /pseudo
     CDS             complement(18435..18737)
                     /locus_tag="ACHKAY_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(19012..20031)
                     /locus_tag="ACHKAY_003269"
     CDS             complement(19012..20031)
                     /locus_tag="ACHKAY_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFO5701897.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            complement(20180..20344)
                     /gene="tnpB"
                     /locus_tag="ACHKAY_003270"
                     /pseudo
     CDS             complement(20180..20344)
                     /gene="tnpB"
                     /locus_tag="ACHKAY_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(20341..>20523)
                     /locus_tag="ACHKAY_003271"
     CDS             complement(20341..>20523)
                     /locus_tag="ACHKAY_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5701898.1"
                     /translation="PTRPVTNPFWRNKPDECPECDPGNVPRCELHLKSGVVKLFDPLT
                     PEMLRALIREMKGDTR"
BASE COUNT         5141 a         4905 c         5694 g         4783 t
ORIGIN      
        1 aatatgcaac gactgccggg tgtgcaggct gatatggaaa ttgtgcccgg tgagcagcca
       61 ggggaaagtg atattcttat tacccgtaaa caggataaat actggcgact ggggttttcg
      121 cttgatgatt caggtacccg cagcacaggg cgttatctcg gtggtataac attttctctg
      181 gataatcctt tttccctgag tgacctgctg tatgtttctg ccacccataa ttttccacat
      241 tacgggggta agggcagtaa aaactacacg gcccattact ctgtgccttt cggttactgg
      301 cagttttccg tcaccgccag tgattacgat taccatcaga cggtagctgg tgcggttgag
      361 gattaccagt acagtggcga gagtcagaat ctgggcatgc agctgagccg ggtactgcac
      421 cgtaacggga cgcagaaaac ggtgctgact tacgatgttc aggcccgcag gtcgcgtaac
      481 tacattaatg atacggaaat ccaggtgcag cgccggcaga cagcggcctg gcgacttggg
      541 ctgcagcacc gtcattatat ttctcaggcc acactggatg ctggtgtcag ctggcaacgg
      601 ggaacccgct ggtttggtgc acagccggca ccggaggaaa tcttcggtga ggcaacggcg
      661 ctcagtaaga tcctgcagat gaatgcgtcc tttaatctgc cctttacact ggcagggcag
      721 cccttcagtt attccttccg gtatcagcgc cagctgagca ataccccgct gaccccacag
      781 gaacagtttg ccatcggtaa ccgctggacg gttcgtggtt ttgacgggga gcgaacgctg
      841 aatgccagta acggctggta tatccgcaac gacctggcct ggcgtacgcc actgccggag
      901 caggagttat atctgggaat ggattacggc gaggtggggg gagccggcag tgaatacctt
      961 gtgggacgtc atctggccgg tggtgttgtg gggctgaggg ggcaactgtt cagtacggat
     1021 tatgacgttt tcatcggtcg accgttttca aagccggcgg gctttatgac gagtgatgtg
     1081 acagccggtt ttaatctgtt ccgggatttc tgagaaagtt tcatgcgtaa cgggaaatat
     1141 gacgttctga gtcccctgta ttccggtgag ccggtcaatg aggcggaagt cctgggggcg
     1201 gccgtctggt tgtggatgca ctcccccctg catcgtgatg cgccactgca taccctgccg
     1261 gacctgctgc tgccggtgat taagcaccga cagtatgtgg tggcaacaga gcaggggcga
     1321 ccggttttct tcatgagcca ggcctggctc agcccggagg cagaggcgcg ttttcttacg
     1381 cagccggcaa ttcttatgcc tcagtcggac tggaacagtg gcgaccggat gtgggtctgt
     1441 gactggattg ccccttttgg acataccccg gatatgagtc gtcttgtccg gcaggatatt
     1501 tttccccgtc tgtgctggcg ttcgctgtac caccgtgggc agcagaccgg gttacgggtg
     1561 atgaatttcc ggggggcaca ggtcagtcgt gaagaggcgc gccagtggcg tgtgcagcac
     1621 ccgcttgctg tggatgtgcc ggagagataa gtcaggttta tcagaatgaa caaaaattgt
     1681 taccgcatta tttttaacaa agcccgggga atgctgatgg tggtggcaga tattgcccgt
     1741 tccggtcgcg ccggtgcatc accctcctcc cgtacaggat atccacgcag gcaacgtatc
     1801 tgccgtgtca catcgcttgc attctccctg tggctggcct cggggatggt gcattctgtc
     1861 agtgctgcag gcattgtcgc agaccatggc gcaccggggc atcagcagcc cacgattatg
     1921 cagacggcca gcggcattcc gcaggtgaat attcagacgc cttctgccgg cggggtttca
     1981 cataacacat acagccagtt cgacgtcggt aacaagggtg tcattctgaa taatgcccat
     2041 aacaatgtgc agactcagct gggtggcatg gtggccggga acccgtggct ggcgaaggga
     2101 gaggcccgaa tcattcttaa tgaagtgaac agtcgcaatc ccagccagtt gaacggtttt
     2161 gttgaggttg ccgggaaaaa ggcgcaggtg gtgattgcga acccggccgg catcagttgc
     2221 gacgggtgcg gttttattaa tgcgaaccgg gccacgctga ccacggggca acctcagatg
     2281 aaaaacggca gtctggccgg attcagtgtt gaacgaggtg agattcagat tacgggcaaa
     2341 gggatggatg cctcgcgcac ggattatacc gatatcattg cccggtctgt gaagattaat
     2401 gcgggtatct gggcgcagga cctgaaagtg accacaggac gtaacaatgt ggatatcgcg
     2461 cacgggcaga ttgagaaaaa gaccggagat gtctcttcac agcctcagat ggcactggat
     2521 gtggccagtc ttggcggaat gtatgccgga aaaattcgcc ttgtcggtac ggaaacgggg
     2581 gtgggggttc gtaacgccgg tcatattggt gcgcaggcag gggcggttac cctgacggct
     2641 gacggacgta ttgaaaacag tggcagtatc agtgcgaaaa cggatgtgca tctggcgaca
     2701 acccgggagc tgcataacag tggttctgtt tatgcggggc aggatacgca gatacagagt
     2761 gatggtgtat tcacccatac aggctctgtg gcatcacgtc gcaacacccg gattcagaca
     2821 gcgcgtctga ccggcggtga gcggagtctg ctggccgccg gggtgaaaga tgacggacgt
     2881 ctggcggcag cgggcaacct gactgtcagt accaccggcg aactggcggc acacggacag
     2941 gttctgtccg gtggggacat gcaactgaag gggcaggggc tggatttaag caacagccgg
     3001 atacagggac aacatactga gctggatgca ggcagtggaa atctgtccac acagaatgca
     3061 caactgagtg ccggtacgct ttccgcccgg actgccgggc agttcagcaa taatggtggc
     3121 acggtaaatg ccgacacact gcagatatcg gcacagagcc tgtcaaatcg taaaggcagt
     3181 ctgattcaga cgggaacagg ggatttttcg ctgagtctgc cgggacgcgt ggataaccgg
     3241 gaagggctgc ttgcggcaaa tggcgcggtg cgcctggatg cactgagcct tgataatcgc
     3301 agggggaaag tgcaggcggc gcagtcaggc agtctgcagg tgaagacaac cggtgccgtg
     3361 gataaccagc agggacgtct gcttgccagc cgtgacatcc tgattaatac acaggcgctg
     3421 aataacgaca acggactgat ttcagctgct gccggaacgg gcaggataaa aacacagcag
     3481 gctgtcagca atacggaggg acgaatggag tccgccggca ggctgggtat ttcggcaggg
     3541 agtctgaata accaccaggg tacggtggtg tctgacgggc tgtccgtgac gctggatggt
     3601 gcgctggata atacgtccgg cagactgctt tcacagaaaa cgctgtctgt gtcaggcagc
     3661 gaactggtct ctgatgatgg tctgattcag tccggcagtg acatgacgct ggatgtacag
     3721 aacggggttc tcagtaaccg gaacacgaaa acacggggtg gtatcagcag cgccggtacc
     3781 ctgacagtac gggccggtat gctgaataac cagcagggat ttatggcggg tcagaaggat
     3841 atgaccctga acgccggaac gcttgataac cgtcagggcg tgctggggag tcaggcgtca
     3901 ttacagatat cgtcaggtac gctgatgaat cagaaaggtg cactgaaggc cgggacggat
     3961 atgttgctga gtggtggcga tgtcagtaat caggaaggga cgcttgccgc cggcggggac
     4021 ctgaatgcgc acctgaatgt gctggaaaat cagcagggga cggtggtcag caacggtaac
     4081 agcaggctgg atgtcacccg ctctgataat cagggcggca gactggtggc acagcagtca
     4141 ctgacccttt cctcgacgga tatcagcaat gatgcaggtg gtctgataca gagcggtgcc
     4201 acactgaatc tccgtgctga cacactgagc aaccgtaaca gtggtgaccg gggcggggtt
     4261 atcagtcagg ggccgatgac gctgaatgcc ggcacactgg acagcactgc cggtgtgctt
     4321 ttgtcgggag atgctctgtc cctgactgca ggcgtggtta acaatacatc cggtcaggtg
     4381 gtggcaaacg gtctgctcgg ctggaacagc caggcgctga acaatcagtc aggtcttatt
     4441 caggggaaag gcatcagcat taataccgca ggccagacgc tggataacag aggcggtacg
     4501 ctgaacagcc tgcaggaact gactgtcagt accggagcaa tggataaccg gggcgggacc
     4561 gtgggggcga aaacgacagc tgacctgagc accacctcgc tggataaccg tgagggtggc
     4621 cggctggtca gtgaaggtga actccgtctg catacgggag gcctgcagaa cagccacgga
     4681 cagatacagt ctgtgggcga cattctgttc gacagtgtac ggggcgttgt ggacaacgtc
     4741 tctggtctga ttcgcagtgg ttctgccatt acgctgaacg ccctgcagtt tatcaatcgc
     4801 cacacgcaga ataccggcca ggggctggaa gcgcagacaa tacacatcac gacgcaggac
     4861 cttgataacc tggagggcag tattctggca gacagggcgc ttacggttat ggcagacagg
     4921 acgctcagta ataatgatgg cgtgctctct tccggtgcga cattgtctgt cagtggccgt
     4981 cagctgacgt tttcgaaccg tgacggcgtg gttaaggccg ggcagtcagt atctgtggat
     5041 gccggtcagc tcggtggtga cgggaaactg ctttcgctgg gcgatatgac gctgaaaagt
     5101 aacacaacgt tcagcaacag tggtcagact attgcgaacg gaaaccttac gctttccgtt
     5161 aatggcgatg tctctaatac cggcagcctg ctggcaggca gccggcttga cctgaacagc
     5221 atccgccttg aaaacacaga gaaaggtgaa atcagtgccg ggcagacctg gcttaacgtg
     5281 acggacacac tgctgaaccg tgggctgatt gacgggaaat atacgcgtct tcaggcgaac
     5341 accctgacga attccggtac cgggcgaatc tacggcgatg ctgttggtgt cagcgctgcc
     5401 acattgaata atctggatga aaatggcgtg gcggcgacac tggccgggcg tgagcgtgtg
     5461 gacctggggg tacagaccct gaataaccgg acgcacagcc tgatttacag tgcaggtgat
     5521 atgcatatcg gtggcacgct ggatgcgaat ggcaccgcca caggtaaagc cggtgtactg
     5581 aataatcaca gtgccactat tgaagccgcc ggatatctgg cactgagtgc cggacaaatc
     5641 aataacgtca atgaccattt caccacggag cgcgtggtgg tttccacgga aaaggtgact
     5701 gagtatcagc atcgcggatc agatgtccgc tggaatgcaa aagatgaggg ggtgttcatt
     5761 gataacaact ccactgatgg tctgctgaac ctgaacacgc cggatgatac cggacataac
     5821 aacgaccggt tcacaaaata tgaatacacc cgtaccgtgg aggaaacccg ggtaaaagac
     5881 tccgacccgg ggaaaatact gtccggtgcc ggcatgacgg ttgtggcaga caaactgctt
     5941 aatgacaaga gtcaggttgt tgcaggtggc ctgctggata tgcaggccgg tgatgtggaa
     6001 aacgtcagtg tcagcggaga gcgccatgtt actgataaag ggacatccac atattattac
     6061 cggataaaaa gaaaaaataa aaatgacagg cagggggaaa gtatttcccc ttacgagccg
     6121 cccaccgtca ttcagaccat cacgctgaag ccgggagagc tcaccagcca cggacaggtt
     6181 cagggcagcc aggtcacgct ttctcccctg aagccacagg gaacggatgt acaggcagga
     6241 ctgaccggaa atgtggatgc gacagtagcc gctactgacc ggataccgct tcgcccggtg
     6301 gtgtctgccg gtgaaccggt cattctgttg ccggggcagc agtttgaagt cagcgcacct
     6361 cagggcagta tccatgttgc cgggccggat acccgtctgc cggacagcag cctgtttaaa
     6421 acaaatccgg cagtgaatgt gccatatctg gttgaaacgg acccgcgttt tacgaaccag
     6481 aagacgtggc tgggcagtga ttacatgcag aaggcgtttt ctcagaacgg ggataacatg
     6541 ctcaaacgcc tgggggatgg tttttacgaa cagcgtctga ttcgtgagca ggttgtcgct
     6601 ctgaccgggc aacggtacct ggacggatac agtaatgatg aagagcagtt taaggcgtta
     6661 atggatgcgg gtattgcatt tggtaagcaa tacaacctga caccaggtgt ggcgctgact
     6721 gctgagcaga tggcgttact gaccggtgat attgtctggc tggttaacac cacggtgaca
     6781 ctgccggacg gcagcacgca gacggttcag gtacctcagg tgtatgcccg tgtaaaaccc
     6841 ggtgatgtga acagtgccgg tgcgctgatt gcgggtcggg acatggtgat gaagctggat
     6901 ggtgacctgt ttaacagcgg taaactggcc gggaagcaga ccgtgcagct cagtgcagaa
     6961 aacattcaca accaggccgg caccattcag ggagcaaatg tcagcctgac ggcccggacg
     7021 gatattaaca gtaccggcgg gctgctgcag gctacggaca gcctgctggc gatggccgga
     7081 cgggatatca gtctgaccac caccacccgt acggcacagc gcgatgccgg gcagaaccat
     7141 tttgaacgta ccagtatcga cagtgtggcc ggcgtgtatg tgcagaacga ccagggaaga
     7201 ctggtgctgc aggccggacg ggatatgaat ctgacggctg caacggtggt gaatcagggt
     7261 aaggacagcc tgacgcaact cagcgcgggc agggatatga cgctttccac agtgaccaca
     7321 tccgcgcagg acaagattac ctgggataaa aataatcgtc tgtcgcaggg cgtcacacag
     7381 agtacgggca gcacgcttgc cggtaacggt gatgtcacgc tgaccgccgg acgggatatg
     7441 acgtcactgg cggcatcgct gtcagctcag aaaggcctgg ccctgatggc gggtcatgac
     7501 gtgacgctga ccggtgcaca gaatacccgc tcgctggatg aataccataa agtgaccggc
     7561 agcagcggga tgttatcgaa aaccaccaca accacgcatg atgtcactga ccgccggacc
     7621 atgacgggca gtgaactgaa cggggatacg gtcagtatca gcgccggaca taatctgaac
     7681 gtcacgggca gcagtgtggc cggggataac cgtgtctccc tggctgccgg aaataacctg
     7741 aatataggta cgctgacaga aagcaaccgt gaaacacacc tgaaacagga gaaaaaatcc
     7801 gggctgatga gctccggagg tgtgggattc agcgtgggca gccagagtct gaaagtcacg
     7861 gacaccgcaa cagataccac gcagaaaggc agcacggtgg ggagtgttca cggtgatgtt
     7921 tcccttcagg caggtaaccg gttgaccgtc aacggctcag acctgattgc cggcagggac
     7981 atggcactca gtggtaagga ggtcagcatt accgccgcca cagaccagca tgtacagaca
     8041 catacggtgg agcagaaaac ctccggtctg acgcttgccc tgtccggcac agcgggcagc
     8101 gccctgaata cgacggtgga gaccgtacag gcggcgaaat ctgccggaaa cagccggctt
     8161 gaagccctgc agggggtgaa ggccgcgctc tcgggggctc aggccgtgca ggccggacga
     8221 ctggcagaag cccagggcaa ccagaaagag aataacaatg ccgtgggtat cagcctctct
     8281 tacggcagcc agtcttcaaa atcagaacag cagtcagagc agacggtggc gaagggcagt
     8341 acactgaccg caggtaataa cctcagtatt caggccactg gctccggcgt gaagggcgtg
     8401 gatggtgacc tgacgataca gggcggtcag ataaaggccg ggaacaacgt tctgctgcag
     8461 gcaaaccgtg acgtgaatct ggtatcagca gaaaacacgt caaagctgga aggaaagaac
     8521 acatccagtg gtggcagcgt gggtgtcggt gttggtgtgg gctccggagg ctggggtatc
     8581 agtgtttcag ccagcgcaaa ccagggtaag ggcagtgaaa aaggcaacgg taccacccat
     8641 acagaaacca cggtggatgc gggaaaccgg ctgaccatta tcagcgggcg ggacaccacg
     8701 cttaccggcg cgcaggctgg cggtgaaacg gtgaaagtgg atgccggtcg ccatctgaca
     8761 ctgaccagtg agcaggacag tgaccgttat gacagtaagc agcagaatgc cagtgccgga
     8821 ggcagcatcg gcaccggtag tgcctcagcc agtgtgagtc acagccggga taaaatgcac
     8881 agcaactatg acagcgtgca ggagcagaca gggatttttg ccggcagggg cgggtttgat
     8941 gtcaccaccg gacagcatac gcaactgaac ggtgcggtga ttgcgtccac ggcaacggca
     9001 gataaaaacc ggctggatac cggcacactg ggcttcagtg acattgaaaa ccgggccgac
     9061 tttaaaacgg aacatcagag tgcagggctc agtaccggtg gcagtgttgc cggaaatttc
     9121 ctggggaaca tggcaaacaa tctgctggtc ggtgcaaatc atgaaggcca tgcggacagt
     9181 accacgcagt cggcagtgtc tgcagggaat atcaccatcc gggatacgca gagccagaaa
     9241 caggatgtgg cagacctgaa ccgtgacgca gcacatgcaa accagacgct gtcccccatc
     9301 tttgacaggg aaaaagagca gcagcgactg cagcaggcgc aactcattgg tgagattggt
     9361 aactaggtgg cagatattgc cagaacggaa ggacagatag ccggtgagaa agcgaagcgg
     9421 gacccggcag cactgaatca ggcccgcgca gagcttgagg cagcaggaaa accgttcacg
     9481 gaacaggatg tggcgcagcg ggcctacaat aacggtatgg cggcctccgg ttttggaacg
     9541 ggaggcaaat accagcaggc gatacaggcc gcgaccgcag cggtacaggg actggccggc
     9601 ggtaatctga gtgcagccct ggcaggtggt gcagcaccgt atctggctga agtggtgaaa
     9661 accatgacca cagacccggt tacaggtgag gtgaataagg ctgcaaatgt ggctgctcat
     9721 gctgtggtga atgccgcact ggcggtggca cagggtaata acgctcttgc cggtgccgca
     9781 ggggctgcca ccggcgaggt ggtgggaatg atagccacac agatgtacgg taagccggtg
     9841 agtgaactga gcgagacaga gaagcagacg gtctcgacac tggcaacggt tgctgccggc
     9901 ctggccggtg gtctggtggg agactcgggg gcttcagcgg tagccggcgc acagtcaggt
     9961 aagacgactg ttgagaataa ttatcttagc gtgtctgaaa agacagagct tgagatagca
    10021 aaacagacgc ttaaaaacag taaagatccg gcagagcgcg aaaaggcgca gcagaaatat
    10081 gatgcattgc ttgaaaaaga tatcagcagt gataaggcgg taatcgctgc atgcagcaac
    10141 ggacaggcag ccagcgcggc ttgtgcaggt gaaagactga gggttattgc tgctaaaggc
    10201 ggttatgaaa ccggaaacta caataaccaa gccagcgaca tgtatccgga tgcttacggg
    10261 cagatagtaa acctgctgaa tatcaccagt gttgatgcac aaaatcaaca acaggtgaag
    10321 gatgcgatgg tgaactatgc catggggcag tttggggtcg atagggcgac ggcacaggca
    10381 tacgttgaaa cttatgatgg aatgaaggtt gtagctgctt cgatggcacc agtaataggt
    10441 gctgcggcgg ccagtaagat agaagttctg gctggtaaac aacgcttgtc taatagtttc
    10501 gaggttagct cacttccaga tgcaaatggg aaaaatcaca tcacggctgt aaaaggtgat
    10561 gctaaaatac ctgtcgataa gattgagcta tatatgcgtg gtaaagcttc tggtgacctt
    10621 gattctttgc aaacagaata taattcttta aaagatgcaa ggattagcag ccaaaaagaa
    10681 tttgcaaaag atccaaataa tgctaaaagg atggaagtat tagaaaaaca aatccataat
    10741 attgagcgtt ctcaagatat ggctcgtgta ttagagcaag caggtatagt gaatactgcc
    10801 agtaataaca gcatgataat ggataagtta ttggactcag ctcagggggt tacgtctgca
    10861 aatagaaaaa catctgttgt tgtatcaggg cctaacggta acgtaaggat ttatgctaca
    10921 tggacaatat tacctgatgg cacgaaaaga ctttcaactg ttaatacagg ggcattcaaa
    10981 tgataaatgt gaatagtatc gcaaaggata ttgaaggttt agaaagttac ctgactaatg
    11041 gctatgtaga ggctaactct tttaatgacc cagaggacga tgcgttagag tgtctcagta
    11101 atttattagt taaagattcg gagggagggc tgtcattttg taaaaaaata cttaattcaa
    11161 ataacattga tggtgttttc attaaaggtt cggcgctgaa ttttttatta ttgtcagaac
    11221 agtggtctta cgcctttgag tatttgacaa gcaatgcgga taatataact cttgcagagc
    11281 ttgaaaaggc gcttttctat ttttactgtg caaaaaatga gacagaccct tatcctgtac
    11341 cagagggttt attcaaaaaa ctaatgaaaa gatatgaaga actgaaaaat gaccctgatg
    11401 caaaatttta tcatcttcat gaaacgtaca atgatttttc aaaagcttac cctctgaata
    11461 attagaagct atcgccgtgg aatattgtct atgactgcat ggaaagatga tggtcatagt
    11521 gtgttgggag ctattcttta acagacttgg gggaaatggt gtaactggct gaaagggcgc
    11581 gtcagggcaa aaagttgttg cgccggaatt acagcaggac aaagcggctt taactcgctg
    11641 aaagcacaga gaaaactggt tttatcacag caacctgcgg cggaaagagt tgttgttgag
    11701 aataacctgc tgggtggcag tgaattgctc cagacggaaa aagccaggga gcatggagct
    11761 gatgttctct cctgtagtga taatccttcc ggtgaagcct gtaagcgggg gcaggctgaa
    11821 aataaagctt atgcagccgc attagcaaca ggcagcgttg ccctgttacc ggggagtgcc
    11881 caggctatgt ggttacttgg tgtcgggaca aatgccggga tgcagtatgc agatagtggt
    11941 gaaataaacc cggtcaactc agtggcagca ggctggataa atgtgataac catggggcag
    12001 ggctggaaag ggacaatagc ctggaatgcg gcaggtggag cattaaccaa cgccataaac
    12061 ggagatgatc ctcttactgg ggcgattaca aatggagcgg gggccgggtt tggatatggg
    12121 gttggcaatt atgtggtcaa gcctgctgca aatacaatcg gtaaatggat aaccggtggc
    12181 tggaatccta aattcgatcc gactctacta aaatatactg aagttaaagg gcagttaggt
    12241 ctatctaaag aaatggtacc gagtaagatc cctggtgctg tgggtaatgc gggtggtagt
    12301 ttatcatctg agtttggaag ctccattatc cagaaaaaga aagatgcaat ggaggctggg
    12361 aaatgaagaa ccttcttatt gttgtcttat taatgattgt atgcatattt ggtttgttta
    12421 tagtcggttc gattttttat cttcttctgg agatatttat gtatttttat ttgaatgccc
    12481 caatatcatt tgaagttttt cagttttcta gactgttaaa aatgagtgtg tatggggggg
    12541 ggatcttagg cttgggtatt ggtttgttgc atatcatgaa agttaagggg ttctgaaatg
    12601 ttgttgagaa taactacctc agctacgacg agacgcatgc ttttgacaga gaaatgacgg
    12661 tctgtcgtaa agcgggcgga gactgtggcg atattcagaa taaatatgct gtattaaacg
    12721 cagagagccg acatcaatta caccttgatg tggccgcaga tcctcttacg gctctgtccg
    12781 gggaagataa atggaatatt gagggcgggc tcagcgcggc gggccgactt ggctggctgt
    12841 atggttcact gactaatcag gaggtgaagg attacgtggc tgaaggcaac agctacgacc
    12901 tgaactatct gaacagtaac acgtcgaagg gtgacagggc tctggctttc ttcggggaac
    12961 cggaaaatta ctggggtacg gttgccggag cgggttcgtt gttaatatca tcagcccacg
    13021 ctgacggaaa aatttgttag tgcggggctg agctatgggg ctaatggtgc ggtacaaatt
    13081 ggtacgggaa atatcggtga taagttcgat tacctcagtt tcatgatgtc aggcttgact
    13141 ggtgtaggta catccgggaa aggatattac gctaaccagt tgttgggtgc tggtagtgct
    13201 tacatgagca accagattga aggcctagac aatacgtatc taaaactatt agttttaatt
    13261 tcattaaaat aacgcttatt aacaatggag ataacgaagt gaaaacattg tctgttattt
    13321 cagcaattat attatctgtg gcaagtggct ccgtgctggc tgacaaccat tcattttctg
    13381 tcggttatgc acagagtaag attcagaact tcaagaatat cagaggggta aacctgcagt
    13441 acaggtatga atgggactcc ccggtcagta ttatgggcag tttctcttgg atgaaagggg
    13501 atggtaatca ggaatatcac tcatatggcg attcaattaa aaaccaggtt gatgcaaaat
    13561 attattcctt actgaccggc cccgtatggc gcattaacga ttatatgtca ctgtatgcgg
    13621 ctggcggcgt ggcgcatgtt aaagctgatg gtaaaacagc atggacaaac gttgatggtt
    13681 atttcgccag agaaaacatt tcgaaaaaat ccacatcttt tgcttatgct gccggggtaa
    13741 tgttcaatcc ggcaagtaat attacaatta atgccggata tgaaggaacc agagcaaaac
    13801 tgggcgataa ttattccatt aatggattca atattggcgt gggttattgc ttttgatgag
    13861 cctgtttacc attctgtgta aatgtctttt cttaaaagtg accgtccagg cggtcaccaa
    13921 actcgataat aaagcggctc attgccattc gccagtccct taacggcatt gcccatttct
    13981 gtgacgcggc ctggattgcc agccacgcca cttttttcac cgagtcgtct gtcgggaaca
    14041 ctttacgttt tttgatcgca tggcggatca cgctgtttag cgactcgatg gcattcgtcg
    14101 tatagatcac tttgcggatg tccgtcggat aagcgaagaa cgtggcaaga ttcggccagt
    14161 tagcctgcca gcttcggctt atctgaggat agcgactgtc ccagaccaca gcgaacgctt
    14221 ccagtgcctg cagacctgcc tcttatcagc gtcatacagc tctttgaacg cggcgactat
    14281 ctcacgggtg gtcatccctt tggcatacaa cgagaggatt tggttatcca tcccggtaat
    14341 acggggctga tttttcttca ccagttgtgg ttcgaaggta ccgtcacgat cgcgcggagt
    14401 acgcagttcc agtggaccgt cgcctgtgat aacggtcttt gtggaataac cattgcggga
    14461 gttagttccc agtctggact gatttttctc atacccgaca tgatgagtca tctctgcatt
    14521 gagagtggct tcaacgctga tctttttcag caaacggtca agctggctga ggtcttcagg
    14581 ggttttgagg tttttggcca gttcgttagc cagagcctgt aactgttttt cgtccataaa
    14641 ttaaccttca tttgatgctg gattgaacat atcaagatca ggcaattaca caaatctatg
    14701 tacaggctcg gggaattggg catctctacc gcttacgtcg agccgctcac acccggtcaa
    14761 tgacctgaca atctcccggc aagtcccgac gtcgggactt gcgcccctga caccgaaaaa
    14821 tagcaggaac ccgctcgcta cgggtcctca gctcagtgca aacctgactc agctcctgca
    14881 ttacatcccc gaaattacca cactcagtac ttcatcacct gccaccaata cccgtgtcag
    14941 tccttcgtga cctgacgcaa ttacctgact cagagcttca gagtcagccg ccggtacctg
    15001 cccggcgctc gcccctgcgc aaccaagata aatcgaccat tggcagactg cgatcgttcg
    15061 tatgcatcgc tgatgacgcg cccgaaaaac gcgtcaatat gagttcgccg gaacgattct
    15121 gtcaaacttt acagtttaac tatcttcgcc aactctgccc gaatgcaaga actacttgca
    15181 tgcagcttac cagttaaggc tagttgacga cggctagcaa gctaaattta atcgtatgtc
    15241 accaatcaac aaatgcgggc ccaagtgagt tacgtcactt tgcaccttgt gctaccgtac
    15301 acaaatcctc aatatcttca gcagaaagtt tatgatgttt aagttgccta tgaacgattt
    15361 gcgagtcggc atacccgcca ttactcgcca acgagattga atatgcttgt atcgcatcgc
    15421 gcatcctatt gagtccttgt tcgttaacta ttcggatgtc ggaatggggc gatgccgcgt
    15481 gttcaaatat ggttttagga tgcaccaaga tgggagtcct tgtacaagtg tgaccgtact
    15541 tttcatcaaa ccagacgatg gacccattta actggttcgt gtcatgtttg gagattcttt
    15601 cagccaaaac agctccgctc ttgcactcaa tcacgaaata cctcgagttg ccaagagccc
    15661 aaagattatc tggtcctctc cccactaggt cttctggcct ctcggatccg aaaccgagaa
    15721 aaatgcctaa ttccttaatg gcagcttcaa accgcttcga cccttcttct ccccattgta
    15781 gatcgtctaa caacccgttg acccatatga tgagatcgtt tccttcaaga aaacgtgtca
    15841 tgaactgaac ggctgcggcc gcttgtccgc tagcgggtgc gctgagacgg tgataagtcg
    15901 ttcctgccaa gggtttaaga agtcgatagt tatcgctgag cgcggccagt tgcagttcct
    15961 gcgcctgtga agggtcagtg aaattcgtat acgccgctaa ctgttgcttc agatagcctt
    16021 tgatctgacg ctcgcttgct gcattcactg ctgtctgagc cttattctta gcagtgtcat
    16081 atcgcgacac ccgggccgca tcgaaggcct cccgcaacag aacctgatta tcgtcaatcc
    16141 gtcccccttc gacaggtggc gcattcacaa ccgcattacg gccagcctgt acccagttgt
    16201 catcttgggt caggcatagg cccataatct cctgcagctt ctcaagcgat tgtccacgca
    16261 actgctccgc tacactttta ccgagctcaa tctgagcacg agtggctgaa gaaaacatcg
    16321 tttcagcctc gggttgatgg agtcgttgcg tcaacctgcc accaagtaat agtacgacac
    16381 aatgatcgtc actagaacgt acaccacgac ccattccctg ttcaattctc tgaacttggc
    16441 gcacaagttg gcgtttggtt ccgtctagca gcaacatttc acgcctttca ctgagtccgt
    16501 aaacctccgg caagccatca atgacgagca actcacaagc ctttcctggc aaatcgatac
    16561 cgtcgtattt attaattagt acggttaggc ctacaaaacc gtccttgaga cgctgtattc
    16621 cagcctgaat gttatctttg ttaagtatct gattagcaga atctttccag aattttgcgc
    16681 gttctttaga ggggacgata acaactacgt tcagatcctt tgcgatatct gacacaagtt
    16741 cgcggatctc ctccattttg aagtccggat tgatctcctg tggggctaga atcattcggt
    16801 ctccaatatc tccgccccct ttaggtcgga tcggcttaac tacctcgtcc gccaatgccc
    16861 caaagtgact cacgaggatc gtatcgtcag ctaaggtggc cgtcatatag attcttcgct
    16921 gagcagttgc gaaggatgga atgcggtcga tcggaatgaa atacggcgcg atttctagtt
    16981 taccgccgcc gaaaaggcag gtgcagagcg ggatacaatc gcgcaacaag ggccaactcc
    17041 attccaactc tgcagaattc cgttgtgcat gaagcaacgc gattacttcc ttttgtttag
    17101 actgccaagc ccagtatggc acttccatta caatgctagg atctagagcc tgtatgtcga
    17161 gcaatccagt gggggactgt ttatccaagt cgttcttaaa aaggttcaga aggtcgtcgt
    17221 acacaggtgt gccaacatct gcctttatcg agaactggtc gtggactacg ctaagacatg
    17281 catgtgcatc atcgacaaca acacagccta tcgggatctt tttctgtccg accccaaaaa
    17341 ccgatcttgc attgaatagc ttccacacat tggcaacaag aatggcacgt ccagcaagga
    17401 aatctggatc cttctcattt tctgttgcgg gaatgccgag cgccttagcc tcatctagta
    17461 cctgattgac aaggaaacta tcgggcgtaa tgtatacagc gggcttgacg ttttcgttga
    17521 gggcgctctg caagcacagc aggcccacca cagtcttacc accgccggta ttcatcttca
    17581 gcgtcaaatc ttgggcctgc ctccgtgcat accaaacgtc aagtacgtcc gcttgttcat
    17641 ccaatggccg cttgaaacga gcgtctcgct taagcgtcgt aaagattttt ctcgggtcag
    17701 tttgttttgc accgatacca gcgttcagct tgtcaaagtc taggctcatg taataatctc
    17761 aagttaagat tgggctatgt aagtccccaa tatttctata atgtattaca atgaccatgc
    17821 aacgagatag tcattgcccc aattagaact ttaagacact ctgtttctag attcattgaa
    17881 ccactacaaa ttttgttaaa actctacaag attgttacga taattccatt cattataccg
    17941 acagtttgtt gggacaggtt ttcgctatgc tggaaacgcg ccgggcatcc gtcatgtatt
    18001 tttcagagca cggtctggag tatgatccga caaaagaaca tgcctatttt catggcggaa
    18061 taaaacccag ccagcaggct tatcatgttc cgatgtttat ctggtacagc ccgacattgg
    18121 gagagcatgt cgatcgccag acggtgaaca gcgttttttc cactgcgtat aatgattatc
    18181 tgattaatgc atggatgggc gtaaccagac cggctcaacc gaaaacgccg gaggaggtga
    18241 ttacccgctg gcaggggaaa tcagtggttt ttgatgccag tcataacatg tttgactaca
    18301 aagtattacg aaagaacttt aatgaaaccc tggagtaatt cattaaagta atttatttcg
    18361 ctatcctttt tccagttact cacaaagaaa aatattcata aaataattcc caggctggca
    18421 gcccgggatg attactattt taatggattg ttaatctttg catattcaaa cgagctgata
    18481 cattgttctc tgttcgcatc cagaaaatct gcccaccact gtagcatcag tcgccgttct
    18541 tccagatgtt ctgctttatg gatatacgca gcacgtacac cattacgctc ctgatggctc
    18601 atctgacgtt ccacagcatc gcgggaccac aaacctgatt caatcaatgc actgcacgcc
    18661 attgcccgga atccatggcc acagacttcc taggttgtgt catatcccat aaccctcagg
    18721 gctttattta cagtatttta tatgaacgca tcccgtttgc aaggcatcca ttgttttgac
    18781 atgacgggta ggttgcagct ttatatccgg ctttattgta gccgtaacgc tgcgagcccc
    18841 tatgtaattc gccgactcac cccttatttt cacggcggac ttcaagccca aaatactgtt
    18901 caggtttagt gagtcacggt cttacctgtc tttgtcatca ctctggccgc catagcaacc
    18961 tttcagcatt cacccttttg ttaagagttc attgtttaat ggtatcagcg attaatatgg
    19021 tctgatactg acgtggttcc tgtcatactt acggtcatgg gcggttatcg cccatactgt
    19081 tcgtgccagc ttgtttgcca tggcgacgat tgccacactg gctggacgac gttttttcag
    19141 ttcggttatc cacgggccag gctctttagc tactaatgcc accgctcttg caccgtggat
    19201 aaataatgtc ctgagataag tgtcaccacg tttgcttatc cccagcagac gtactttccc
    19261 tccggagcca gtttgttttg gaaccagacc aacatatgcg gcgaactctc gccccgattt
    19321 aaacgctgat gcttccccca tggtggccac cgctgccgtc gcaataagtg gtccaacgcc
    19381 aggaatatcc agcaaccgct tacaggtttc attctgcctc gccacgctgg taagctgttt
    19441 ttcaatatcc tctatcagtg agtccagctc attcagtcgg ttgtactggt tttccagaac
    19501 cataatgaga tacggtggca gtctctcttt catccgttcc agtgcttcgg ggaactcccg
    19561 ctccatcgct gcccggcctt tgtggatggt ttcaccaaac tccagtaacg tcccgtgcag
    19621 ggcattaatt tgtgcggtcc ggaacttcac cagttgcatg cgggtacggt gcagaaccag
    19681 taccgactgc tgttcttctg tttttacggc gatttcttta cccggctgct gaaccgccat
    19741 ccagatagcc cgggcatcca tcacatcatt cttattgccc atgacgaatg ccttaacgaa
    19801 gcgggcttgc aacaaccgga ctttatgacc aagttttgtc agttcccgtg cccagtgctg
    19861 agaacctcca caggcctcca taccaatcag gcatggctca cggttgccga agaacgtcag
    19921 aaaatcctgt ctacgcaact gtttatcaac cacttcacct gtgtgctcat tgatgaagtg
    19981 aatctgaatg acatgttttg cgatatcaac gccaaccggt gtatatttca tttgtggagt
    20041 ctccagtctg agggagcttt atgcatcccg tattgggcac tatgatgccg gaaatctgcg
    20101 aggctccaca cctgccagtt tcaccctttt aaccatcata ctgctccgtg tttaattgtg
    20161 tgggatgcgt tcattacatt ttcaccattt tgccactgcg gccccggaag acgaagatat
    20221 gcccggagaa cgggtcatct ttcagcgtgt tctgcacctt cgaagccagg ccgttgaagc
    20281 cacaacgcat atctgtgatg ccagcgatga tccagattct ggtaccggtt ggcagcgtta
    20341 tcatcgggta tctcctttca tttcgcggat tagcgcccgt aacatttccg gagtgagagg
    20401 gtcaaacagt tttaccacac ctgatttaag atgcagctcg caccgtggga cgtttccggg
    20461 atcacactca gggcactcat caggcttgtt acgccagaag ggatttgtaa ctggtctggt
    20521 cgg
//
LOCUS       DAZFKM010000087        20476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFKM010000087 DAZFKM010000000
VERSION     DAZFKM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(166..912)
                     /gene="cutC"
                     /locus_tag="ACHKAY_003272"
     CDS             complement(166..912)
                     /gene="cutC"
                     /locus_tag="ACHKAY_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO5701899.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(926..1492)
                     /gene="yecM"
                     /locus_tag="ACHKAY_003273"
     CDS             complement(926..1492)
                     /gene="yecM"
                     /locus_tag="ACHKAY_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO5701900.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1708..3441
                     /gene="argS"
                     /locus_tag="ACHKAY_003274"
     CDS             1708..3441
                     /gene="argS"
                     /locus_tag="ACHKAY_003274"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO5701901.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3483..3527)
                     /gene="yecV"
                     /locus_tag="ACHKAY_003275"
     CDS             complement(3483..3527)
                     /gene="yecV"
                     /locus_tag="ACHKAY_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO5701902.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3618..4106
                     /gene="yecT"
                     /locus_tag="ACHKAY_003276"
     CDS             3618..4106
                     /gene="yecT"
                     /locus_tag="ACHKAY_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO5701903.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4226..4618)
                     /gene="flhe"
                     /locus_tag="ACHKAY_003277"
     CDS             complement(4226..4618)
                     /gene="flhe"
                     /locus_tag="ACHKAY_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO5701904.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4618..6696)
                     /gene="flhA"
                     /locus_tag="ACHKAY_003278"
     CDS             complement(4618..6696)
                     /gene="flhA"
                     /locus_tag="ACHKAY_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO5701905.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6689..7837)
                     /gene="flhB"
                     /locus_tag="ACHKAY_003279"
     CDS             complement(6689..7837)
                     /gene="flhB"
                     /locus_tag="ACHKAY_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO5701906.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8039..8683)
                     /gene="cheZ"
                     /locus_tag="ACHKAY_003280"
     CDS             complement(8039..8683)
                     /gene="cheZ"
                     /locus_tag="ACHKAY_003280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO5701907.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8694..9083)
                     /gene="cheY"
                     /locus_tag="ACHKAY_003281"
     CDS             complement(8694..9083)
                     /gene="cheY"
                     /locus_tag="ACHKAY_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO5701908.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9098..10147)
                     /gene="cheB"
                     /locus_tag="ACHKAY_003282"
     CDS             complement(9098..10147)
                     /gene="cheB"
                     /locus_tag="ACHKAY_003282"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO5701909.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10150..11010)
                     /gene="cheR"
                     /locus_tag="ACHKAY_003283"
     CDS             complement(10150..11010)
                     /gene="cheR"
                     /locus_tag="ACHKAY_003283"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO5701910.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11029..12633)
                     /gene="tap"
                     /locus_tag="ACHKAY_003284"
     CDS             complement(11029..12633)
                     /gene="tap"
                     /locus_tag="ACHKAY_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO5701911.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12679..14340)
                     /gene="tar"
                     /locus_tag="ACHKAY_003285"
     CDS             complement(12679..14340)
                     /gene="tar"
                     /locus_tag="ACHKAY_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO5701912.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14485..14988)
                     /gene="cheW"
                     /locus_tag="ACHKAY_003286"
     CDS             complement(14485..14988)
                     /gene="cheW"
                     /locus_tag="ACHKAY_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO5701913.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15009..16973)
                     /gene="cheA"
                     /locus_tag="ACHKAY_003287"
     CDS             complement(15009..16973)
                     /gene="cheA"
                     /locus_tag="ACHKAY_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO5701914.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16978..17904)
                     /gene="motB"
                     /locus_tag="ACHKAY_003288"
     CDS             complement(16978..17904)
                     /gene="motB"
                     /locus_tag="ACHKAY_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO5701915.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17901..18788)
                     /gene="motA"
                     /locus_tag="ACHKAY_003289"
     CDS             complement(17901..18788)
                     /gene="motA"
                     /locus_tag="ACHKAY_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO5701916.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(18915..19493)
                     /gene="flhC"
                     /locus_tag="ACHKAY_003290"
     CDS             complement(18915..19493)
                     /gene="flhC"
                     /locus_tag="ACHKAY_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO5701917.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19496..19846)
                     /gene="flhD"
                     /locus_tag="ACHKAY_003291"
     CDS             complement(19496..19846)
                     /gene="flhD"
                     /locus_tag="ACHKAY_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO5701918.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4942 a         5607 c         4963 g         4964 t
ORIGIN      
        1 aaaaatatta ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc
       61 gttcgttttt tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg
      121 gtacgagcaa gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg
      181 gcgttcaatg attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata
      241 ctcgtccgcg tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga
      301 ggcttgccac gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg
      361 caggttttct gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat
      421 aagttccata atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac
      481 ccgggcaatg cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc
      541 gaaggcgcga tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg
      601 tggcatatcg acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa
      661 ttcgcggacc gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa
      721 atcaccaccg cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga
      781 tttcagtacc cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac
      841 tctgtctgcg ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc
      901 cagtaatgcc atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa
      961 tggaccacgg atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg
     1021 gtaatcgctc atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga
     1081 gtccttcatc agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccggca
     1141 gaacgatttc aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta
     1201 gctccacaat agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac
     1261 aaatcggtct gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc
     1321 gacgccagcg ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca
     1381 gcggagtgat attcaacccc agacggaggg aaagttcatc taatgcgtgg gtaaatcgcg
     1441 gtaaatccga tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc
     1501 ttcgtctttc agtaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt
     1561 tcacgccgct tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga
     1621 aaacagccat ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg
     1681 ctcgcttcat caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc
     1741 cgtcaggcca tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca
     1801 gcaaaagttc agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg
     1861 ggtatggcac cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc
     1921 gccagcaaag ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc
     1981 ctggctgaac atgttcagca ggcgctggcg tccgatcgtc tcggtgtttc tacgccagaa
     2041 aaacagacca ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt
     2101 cacctgcgct ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac
     2161 aaagtgattc gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca
     2221 tggctggaaa agcagcagca ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt
     2281 ttctaccgcg atgcgaaaaa gcattacgac gaagatgaag agttcgccga gcgtgcacgt
     2341 aactacgtgg taaaactgca aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg
     2401 gtcgacatca ccatgacgca gaaccagatc acttacgatc gtctcaacgt gacgctgacc
     2461 cgtgatgacg tgatgggcga aagcctctac aacccaatgc tgccaggaat tgtggcggat
     2521 ctcaaagcca aaggtctggc agtagaaagc gaaggggcga ccgtcgtatt ccttgatgag
     2581 tttaaaaaca aggaaggcga accgatgggc gtgatcattc agaagaaaga tggcggctat
     2641 ctctacacca ccactgatat cgcctgtgcg aaatatcgtt atgaatcact gcatgccgat
     2701 cgcgtgctgt attacatcga ctcccgtcag catcaacacc taatgcaggc atgggcgatc
     2761 gtccgtaaag caggctatgt accggaatcc gtaccgctgg aacaccacat gttcggcatg
     2821 atgctgggta aagacggcaa accgttcaaa acccgcgcgg gtggtacagt gaaactggcc
     2881 gatctgctgg atgaagccct ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat
     2941 atgccagctg acgagctgga aaaactggct aacgcggttg gtattggtgc ggtgaaatat
     3001 gcggatctct ccaaaaaccg caccacggac tacatcttcg actgggacaa catgctggcg
     3061 tttgagggta ataccgcgcc atacatgcag tatgcttaca cgcgtgtatt gtccgtgttc
     3121 cgtaaggcag aaattaacga agagcaactg gctgcagctc cggtaatcat ccgtgaagat
     3181 cgtgaagcgc aactggcagc tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc
     3241 cgtgaaggca cgccgcatgt gatgtgtgct tacctgtacg atctggctgg tctgttctct
     3301 ggcttctacg agcactgccc gatcctcagc gcagaaaacg aagaagtgcg taacagccgt
     3361 ctgaaactgg cacaactgac agcgaagacg ctaaagctgg gtctggatac gctgggtatt
     3421 gagacagtag agcgtatgta atcgattttt cgtgagagta aagcctgatc agggttacct
     3481 gatcaggctt ttttattgcc atctaaatgt attctgaaaa tggacatgcc attgttttct
     3541 gactgttgga taagaggcca gaagcgtaat atccggcccc agggaaacga taacggttga
     3601 atttaaggaa taccgcagtg tttaaatttc ttgtattaac attaggcatt atctcttgcc
     3661 aggcttacgc agaagatacg gttatagtaa acgaccatga catttcagcc atcaaagatt
     3721 gttggcaaaa aaattcagat gatgatactg acattaacgt gatcaaatca tgcctgcgac
     3781 aagaatacaa tctcgtcgat gcgcaattaa ataaagccta tggtgaagct tatcgttata
     3841 tagaacaagt gccacgcaca ggtgtaaaaa aacctgatac cgaacaactt aacttgctta
     3901 aaaaatcaca gcgagcctgg ctggatttta gggacaaaga atgtgaatta atcctttcaa
     3961 atgaggacgt tcaggattta agtgaccctt attctgaatc agaatggctc tcatgtatga
     4021 tcatacagac caatacgcga actcgccagt tgcagctata ccgtaactct gaagattttt
     4081 atccaagccc tttgacaaga ggataattca catctttttg gcatgttttg ttgcaagcta
     4141 ttcctgataa ataattgcaa caagacatcg agcctttttc actgagttat taaacatact
     4201 cgcgagcgcg taattttttt gtccttcagc gataattcac aatcacttca ttacgttgta
     4261 cctttagcgg tggaattaac cgcccaccgc ctggcacttc ccagataaat cgcaacggtt
     4321 ctgctgccgc tataccggaa aaggccacgg tggttccgct ctgcccctct aattcgacac
     4381 aacgagattg cgaacacaag cgaacccgca gtcctgaagg tgtcgggccg ataagctgat
     4441 aacgccacgc taccagcgtc atcaatcctg aagcaggttg tcgcgtagaa agaggcgcag
     4501 acgacatcga ctcaccgcga tgatttagcg taattcctac actacttgcc tgccacatcc
     4561 cctccccggc ggcttgcacc agcagcggaa acaataatat tgctaataag gttctcatta
     4621 tttgccgcca attgtcgccg tcatgcggat atgtcggtta tcagacagtt ccagattcga
     4681 caggaccact aactgcggca agctgcggcg caggaagcga gacaataatg gtcgcggcgc
     4741 gtggttcacc aacaatactg gcggcgcacc cagcatctcc tgacgggata gcgcttcctg
     4801 agtttgcgcc agtaaacgat ccgccagccc tggctccagt cctcccccgc cctgcagcgc
     4861 ctgtagtaac aaacgttcca gcggtgtatc gaggccaata acatggactt catctttgcc
     4921 aggaaaccac tgctgggtaa tcgcccgtcc caacgccacg cgcacgacgg cggttaattc
     4981 atgtggatcg ctttggatgg gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc
     5041 gcgaatcggt actttttcat cgaggagatt ttgcagcact ttatgcagtg tggtgagcgt
     5101 gacgacgcca ggaacgagat cttccgtcag ctttggcatc tcctgggcga cgcgatccaa
     5161 cagctgttgc gcctcctgac gaccaaacag ctctgcggca tgctggctaa tgaggtggtt
     5221 aagatgcgtt gctaccaccg tgctggcctc aaccactgtg tacccctgaa tctgcgcctg
     5281 ttcttttagc gcactttcaa tccagatagc attcaggcca aatgccggat cgacggtcgc
     5341 ctcaccaggt aacgtcccgg cagcggttcc agggttaatc gccagccagc gccccggata
     5401 agcatcacca ctgccaatct ccacgccttt catcaaaatg cgatagcggg caggttgcag
     5461 ctccatattg tcgcgaatgt gcaccactgg cggcagaaat cccatctcct gggcaaattt
     5521 cttgcggata ctgcgtatac ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg
     5581 gatcagtcga taacccactt ccattcccag agaatcttcc agttgtacat cgttccacgt
     5641 cgcttcgaca acggtattat tctctgccat ttttaccggt ttgggttcga caggcgcttt
     5701 ttgttcgcgt ccgcgtatcc accaggccag ccccagcaat ccggcagtga acagcaaaaa
     5761 taccaggttc ggcattccag gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa
     5821 cataacgctt gggttactga aaagctgatt caccatctgc tcgccaacat cctgatcggt
     5881 gctgacacgc gtaacgatga cccccgcggc ggtagaaatc accagcgccg gaatttgtgc
     5941 caccagaccg tcgccaatgg tcaatagcgt ataactttcc gccgcgtgtc ccatgctcat
     6001 gccatgttgc agcacgccga ccagcaaccc gccgacaacg ttaatgacca tgatgaggat
     6061 cccggcgatg gcatcgccgc gaacaaactt acttgccccg tccattgagc cgtaaaaatc
     6121 ggcttcctga gtcacttcgg agcggcgttt tttcgcctca tcttcaccaa tcaatccagc
     6181 gttaaggtcg gcgtcaatcg ccatctgctt acccggcata ccatcgagaa caaagcgcgc
     6241 acccacttct gcgatacgcc cggcaccttt ggtaatgacc ataaagttga tgatcacgag
     6301 aatgacaaac actacgatac cgatagcgaa attgccacca acgaggaagt gaccgaatgc
     6361 ttcgaccacc ttcccagccg ccgccgcgcc ggtatgccct tccatcaaaa tgatacgggt
     6421 tgaagccacg ttaagtgcca gacgcaacaa cgtagtaaac aacagaatgg tcggaaacgc
     6481 agcaaactca agcgtgcgct gggtaaacat cgccaccagc aacaccatga tcgacaaggc
     6541 aatattgaag gtaaacaaca ggtcgagtat gaatgcgggc agtggcagca ccatcatcga
     6601 caagatcaac aggatcaaaa tcggtccggc aaggatctgc cattgtgtcg atttcaggtt
     6661 tgcgggcagg cgcagcatcg cggccagatt actcatgggt cggtttctcg ttaataaaat
     6721 ccagggcttc cggcaccgga agatgagtag gttgtacagg gcgctgtcca ccagccagac
     6781 gccagcgttt cagttgccag acccaggcca gcacttccgc caccgcggcg tacagttgac
     6841 ccgggatttg ttgaccaatc tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt
     6901 caagcgtcgg gacgttattt tcagcgccaa tttcacgaat gcgcagcgcg accagccctg
     6961 cacctttagc gaccactttc ggtgcgctca ttttgttttc gtcatactgc aacgctaccg
     7021 aatagtgggt cgggttattg acaatgacat ccgctttcgg cacatcggcc atcatccgac
     7081 gccgtgcagc agctcgctgc atctgacgga tccgcccttt aacatgaggg tcaccttcgc
     7141 tttgtttgaa ctcatcacga atatcctgcc gtgacatacg cagctttttc aggtggctga
     7201 atatttggaa aaagacgtca aatcccacca ttggaatgac accaagcacc accagcagtg
     7261 cgcatagccc taccaaatcc atcgcattac ccatggcggt aatcggagac tcggccatca
     7321 agcgcatcat ctgcggccag tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga
     7381 tggttttcaa aattgcttta agcaactccg cgccagtctg agccgagaac atccgtttaa
     7441 tgcccggtag cgggttgagt ttggaaaact tcggctgcaa ggatttgccg ctaaatacca
     7501 gccctcccag catgaccgga gaaatgagcg ccaccagcac cacgccgcta atcagcggca
     7561 gcagcgccag catggcttct ctgatcagca gaataatctg cccgaggatc agattcgggt
     7621 cattgataat actgtgatca aaatgcagcc cagcggagag catgcccgac aatcgacggg
     7681 ccagcgacac accgccaaac cagataacac taacgcccac cagcaaaatc agcagtgagg
     7741 tcagttcacg ggaacgcggg atttgcccct cttcccgcgc tttttctagt cggtgaggtg
     7801 tgggggcttc tgttttgtcg tcgctctcgt cagacacgtc gccaatcctg gaaagagtat
     7861 taagcgtgaa tgatgccaga gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg
     7921 cgggatttag ggcttttgct gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg
     7981 tcatcgcctt atcagaccgc ctgatatgac gtggtcacgc cacatcaggc aatacaaatc
     8041 aaaatccaag actatccaac aaatcgtcca cctgatcctg actggctacc acaccggctt
     8101 tgctggtatc gacctgaggt ccattaagca aactctggtt ttcacgtttt ggacgcgact
     8161 cctgttccgg gatgttttcc aacagcacca tcagcaactg gcgttcgatc tcctgaatga
     8221 catccatcat ccgcttaatg acctgcccgg tgagatcctg aaaatcctgc gccatcatga
     8281 tttccagcag ttgcgcgtta gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc
     8341 gtgtatctgt taccagttca cgggcgtcgg caaggtcaat cggatcggca aaccagtcat
     8401 cccaacgttg ggttaacgct tttgctgatt tctccatttg atcctgatgc ggttgtgacg
     8461 cctcaacact gttcagcgcc cgctccgcag cctgggcggt catctgcaca acatagtaca
     8521 aacgatcgcg cgcatcgggg atggcttccg ccgcttcggc aatggcctga tccagcccca
     8581 gttcccgcaa actgtcgcgc agcatacgcg tcaggctgcc gatgcgcgca atgatatcgc
     8641 cagctgaatg ctcgtcagca ggtttgattg atggttgcat catagtcgca tcctcacatg
     8701 cccagtttct caaagatttt gttgagtttt tcctccagcg tcgcggcggt aaatggcttc
     8761 accacatagc cactggcccc cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct
     8821 gcagtcacca ttaacactgg caatgccgac atcgcgccat ccgcacgaat tgttttcagc
     8881 aattccaggc catccatatt gggcatgttc cagtcggaga taacaaatcc ataaccgcct
     8941 gcctgcaact tattgagagc gtcgacgcca tcttccgctt cctcaacatt attgaatccc
     9001 agctctttca gcaggttacg cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa
     9061 aatttaagtt ctttatccgc catttcacac tcctgattta aatacgtatc gcctgtccgg
     9121 cactaatttt tgccaacatt tgctggctta cctggctaag atcgaccact tcgcagacac
     9181 cacccatatt gatggcctcg cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg
     9241 caagggtcca tgcccccgcc tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca
     9301 taccggtcag gatcacccca accgcattac gccccgcctg tttggcgaca gaatggaaca
     9361 acacatctac cgaaggccga tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt
     9421 aatttgcgcc actacgcgac agctccatat gccgatcgcc cggcgcaata taggcatgcc
     9481 ccggcaagac acgttctccg tcttcggctt ctttaacccc gatctggcaa agcttattaa
     9541 gtctgtcggc aaaagagcgg gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg
     9601 ggctggaaag cggcaacggt tgcagtacgt gacgaattgc ctcagttcca cccgttgaag
     9661 caccaatcgc aatcagtttt tcagaactca acaacggacc cgccttcagc gttgtcggtg
     9721 ctgacaatgg cttatgtgct gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa
     9781 tcatttcgct atacgccagc attccttcgc gaatacccag ttgcggtttg gtgacaaaat
     9841 ctattgcccc cagctccagc gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag
     9901 aaaccatcac aacgggcatt ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt
     9961 ccatccgcgg catttcaaca tccagcgtca gcacatcggg attgaatttc ttaatcaagt
    10021 cacgcgcgac cagcggatca ggcgcggtcg ccaccatttc catgtcgcta tggctgttga
    10081 tgatttctgt catgatctgg cgcatcagtg ccgaatcatc gacagataac accctgattt
    10141 tgctcatcgt taatccttac ttagcgcata caccgtctga ccacgcagcg tgaagcggcg
    10201 ctcaaggtgg ctaaagtttt cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag
    10261 cggaacaaag cggcgcaaaa tctcctgctg ggtattttga tcgaagtaga tcatgacgtt
    10321 acgacagaag atcgcatcaa acggccccgg cacggtgtac tgtttcgcca gtagattcag
    10381 cggggcaaaa tcaacatagt tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg
    10441 tcccgtccct cgcatgaaat accgttgcag ttgctgcggc gtcaggtttt tcaactcttc
    10501 atggcgataa ataccgcttc tggctttttc cagcacttcg gtgtcgatat cactggcaaa
    10561 cactttccag cgtccgggcg cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta
    10621 cggctcttcg ccggtcgaag ccgccgcgct ccatacgcga tactcgccag aacggcgacg
    10681 tgcgtgatcc gcgagcagag ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt
    10741 ggtcagcgaa ttgataaacg cctgccactc accgctgtgc tgattagatt ccagcaagtt
    10801 cagataatga ccgaaatccg tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta
    10861 aaccatgtcg cgtttatggt cagccagaac gatcccggct cgttgataga tcaattgact
    10921 tatccgccga aaatgcgcgt cggaaagcgc caggcgctcg gtcatctgta acaataaaga
    10981 cgtttgccca cagggcagag atgaagtcat agcgccttct caatcacttc aggataccac
    11041 tggcgcaatt tgtaactgcg ccgactcatg tcgtgctact tcatgttctt caagggtaaa
    11101 taccgccaca cgcgacgaaa gatggtcggc ctgattcgcc agttgttccg ttgccaccgc
    11161 cgcttcttct accagcgagg cgttctgctg agtcacctga tccatctggc tgacagcctg
    11221 tgcaacctgc tcaatccccc gctgttgttc ttccgacgca gaggcaattt ctcccataat
    11281 gtcgtccacg cgggtcaccg aactgacaat atcgatcatg gtcgcggcgg cgttattcac
    11341 cagtttcgaa ccctgctgga cacgattgac tgactcttcg atcagccctt tgatctcttt
    11401 tgctgcctgc gcgctacggc tggcaagatt gcgcacttca cctgccacta ccgcaaaacc
    11461 acgcccctgc tctccggcgc gagccgcttc cactgccgca ttcagggcca gaatattggt
    11521 ctggaaagca attccgtcga taacgctgat aatgtcgcca attttttgcg agctggtggc
    11581 gatctcctgc atggtgtgag tcatggtact gacctgaaca ccgcccgcct gcgccgttgt
    11641 cgcggcattt tttgccagtt ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt
    11701 ggcggttaat tgctccatac tggcggccgt ttgtgccagc gatgccgcct gctgttcggt
    11761 acgacttgag agatcgttat tgcctgcgac aatctccgcg ataccaatgt gcatctcctg
    11821 gcttccctta cgcacatcac tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct
    11881 ggcaaaaatg gcggtgatct cattacgacc atataccgca atcggacgcg ccagattacc
    11941 cgcagcaatg ctgtcaaaat ggctaccgat aatggccagt ggttgaacaa tcatcttgcg
    12001 cgtccaccac agcgcactgc tgatgtagat cgccgcaaca ataatcatgc tgataaacac
    12061 cagcgccgaa atctgatagt tacgctggct ttgcgcactg gcggcttcca gcacatggtt
    12121 gatctccagc tgccaggcct caaagttaac gtcaaacgca ttctgtgatt cctgcaccgg
    12181 cgcagtgagg aaatccgaaa gttgattact ttcaagccag gtcgcctgat gttcgagatc
    12241 gttgttgtgc cagcgggcaa aacttttctc cgtctctttt tgcaacgccc tgacgtgctc
    12301 gttgcccgca gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc
    12361 gcgcgccgtc gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt
    12421 gcccgctttg ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc
    12481 cgcacgttgc tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc
    12541 ccaaaaagac atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa
    12601 cagcgtggtc gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac
    12661 cgcgttagta aatactcgtc aaaatgtttc ccagtttgga gcttgttcag caattcgcag
    12721 tcgtggctgt gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt
    12781 gagtgggctg gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc
    12841 ttccagcgca gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac
    12901 gcgatccatt tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga
    12961 tgccgatgca atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt
    13021 cattgtttcc ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg agacggagtc
    13081 ttcaatgagg gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac
    13141 ttcacccgcc accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc
    13201 ggcattcagc gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc
    13261 ggcaattttc ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac
    13321 tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc
    13381 gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc
    13441 cagcgcggat gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc
    13501 acgggtaccg gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa
    13561 agagcgttgc atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat
    13621 ggtcagggta ttcgccaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc
    13681 cagcggagta agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac
    13741 caccagcgcg ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt
    13801 gacgatatcg cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat
    13861 tgcattttgc attccctggg ttggctgagc gaaataagct ccagtattgc cataatcaag
    13921 ataatcaatc agttcagtta acgctgtgtg atagttttta tatttttcat caatattacg
    13981 actggtagcg accatttcag gtaacggtgc catgcttttg aattttttat aatgagtcgc
    14041 ggcctgggcc aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg
    14101 attggaggaa tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag
    14161 cattaaatcc caggttgacg tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac
    14221 aaagctcttc tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa
    14281 cagtgcgaat acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat
    14341 aaggcacctt cctgaaaaca agttaatctc gttatcggca agaagggggg aaactttatt
    14401 gctgatgcca cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca
    14461 tttcaattga ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc
    14521 catctcttcg ctgttcagca gtttttcgat gttcaccaga atcaacatcc ggtcgcccag
    14581 tgcgcccagt ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg
    14641 aatttgctcc gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac
    14701 ccgctgtccg agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg
    14761 gctgaactta attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc
    14821 tttgataaac gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg
    14881 cactttcagg atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg
    14941 gcctgacggc tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt
    15001 ttactcattc aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg
    15061 gagacatcaa caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg
    15121 gggactttgc gatagttact ttcaaggttt ttaaccacaa cctggtgctg accaattaat
    15181 tgatccacca gcaaggcgta gcggcgaccg ccactttgta agatcaccac aattccctgg
    15241 gtggcttcgg ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga
    15301 tattcacccc gcacttccag cacccgctcg ccgccggcca gtggatgcag atcggcttca
    15361 cggggttgca gtgattccat aacagcattc agcggcagaa tgaaaacttc atccgcaacg
    15421 cgtacggaca tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg
    15481 ccagtaccct gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta
    15541 acgacgtcca tgccgacgcc gcgcccggag acgtcggtga cctgctctgc cgtggagaag
    15601 cctggtgcaa atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa
    15661 ccttgcgagg ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc
    15721 acttcaatgc aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg
    15781 tttttacctg cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt
    15841 accaggtggg tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa
    15901 ctgcccacca gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc
    15961 cggggatagc gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc
    16021 tgcaaatcac gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta
    16081 accgggtcca gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg
    16141 acgaggttaa ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg
    16201 ctgcgcgtcg ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta
    16261 agcactggtg gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg
    16321 gcttcaatca caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat
    16381 attgccgaga gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt
    16441 tcttcttcca gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc
    16501 gactgactgc gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc
    16561 actgcggatg gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata
    16621 taatcgaagc tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc
    16681 atgatgtcct tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct
    16741 cgtctggctt catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag
    16801 ccaaaagttc ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt
    16861 tcggcatctg gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt
    16921 tcgtccgctt catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca
    16981 cctcggttcg gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc
    17041 cagggcgctt actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc
    17101 ttgtttgttc agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc
    17161 gcttaagcgc atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc
    17221 tccgaccatc agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct
    17281 atatcctttc tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg
    17341 gttgggaata ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc
    17401 gacatcggca ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg
    17461 tagaccttcc tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc
    17521 ggactctatc aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg
    17581 ttttttcagc tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc
    17641 atcaccaccg ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc
    17701 agtcgccagt ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat
    17761 ggagatcagc cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc
    17821 aatcttccac gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat
    17881 aatcggatgc gcttgattct tcatgcttcc tcggttgtca tctgttgttg cggatttttc
    17941 accgcacgca catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt
    18001 ttgcgaccaa actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg
    18061 actttgacgc actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct
    18121 aatggggaaa taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt
    18181 gcgataagcg cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgacc
    18241 cccattacag ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg
    18301 ttcgccggga cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg
    18361 atttcgaagg tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca
    18421 agcatgacgc tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga
    18481 ttttcaatat cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga
    18541 taaagcagag ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc
    18601 aacggcagcg ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac
    18661 ccaatccctg caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt
    18721 ccaccggtca tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag
    18781 ataagcacga catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt
    18841 aacgcgcgat tggggcaaaa aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg
    18901 cctcaccgta tcagttaaac agcctgtact ctctgttcat ccagcagttg tgggataata
    18961 tcggcaggat tctgggaaag tttacgtctt tttactgccc gggatggcgg ttgacataag
    19021 ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa taaaattgcc gccgcagcag
    19081 ttgcagctgg aaagttgcag taatccactt tcaacaaacc gcaccaatgt ccaggcacgg
    19141 gttaatgcca gcagtggtcc ttcttctgct tgtgggcact gttcaaggta taaacggtag
    19201 gctttgatca ccgcatcgac gccattacac aaaccggttt tcagtaaaaa ctgccatgca
    19261 ttacagaaca tcgaagcatg aacgttttgt tcccaggtca taaaccagtc ggttgagaat
    19321 ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt tataaagttt tatcaggcgt
    19381 ccgcgactta actgtgtttc gctttccagc atctgcaaac gagcgcccag ggtgatcaat
    19441 tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc ttttttcact catgatcagg
    19501 cccttttctt gcgcagcgct tcttcaggct gattaacatc attcagcaag cgtgttgaga
    19561 gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag
    19621 taatcgtctg gtggctgtca aaacggaagt gacaaaccag ttgattggtt tctgccagct
    19681 taaccatttg cggaagagtc agtgccgcta acgttgtcgc catttcttca tttatgccga
    19741 gacgaaacat agcggacgct ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg
    19801 acaagttgat gtcataaatg tgtttcagca actcggaggt atgcattatt cccacccaga
    19861 ataaccaact ttatttttat gcggtttcac cgcaccccgt gatgtcgccg ggaagccccg
    19921 gtaaaaaata attagcatta gaatagttgc gataagctgc aataagcaga accacctttt
    19981 tggtttaata tgtccttaca aatagaaatg ggtctttaca cttatctaag atttttccta
    20041 aatcgacgca actgtactcg tcactacacg cacatacaac ggaggggggc tgcgattttc
    20101 aataatgtgt gatgtagatc acacaaaaca ctcaattact taacataaat gtataagtca
    20161 tacttttgtt ttgggtgtat ttcaatctgt taaaaagttt ttcgctacgc tagcaagcaa
    20221 aaatgaaaca ggaataatcg aaatgggatg ttgcgcacag ttaaaataac tcaccgtaaa
    20281 taatcatctg ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga
    20341 cacttaccgc acaacaaact aattaaatac aaagaaaggc aacaatcaac ttaatgttaa
    20401 tgaaatgttt tacatatatt aaccacttgt ttaatgcgga gaatcagtac actattttct
    20461 tataattaca tttgaa
//
LOCUS       DAZFKM010000088        20288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFKM010000088 DAZFKM010000000
VERSION     DAZFKM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..188
                     /locus_tag="ACHKAY_003292"
                     /pseudo
     CDS             93..188
                     /locus_tag="ACHKAY_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            212..4726
                     /locus_tag="ACHKAY_003293"
     CDS             212..4726
                     /locus_tag="ACHKAY_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO5701919.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4727..6376
                     /gene="yfaQ"
                     /locus_tag="ACHKAY_003294"
     CDS             4727..6376
                     /gene="yfaQ"
                     /locus_tag="ACHKAY_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO5701920.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6381..7157
                     /gene="yfaP"
                     /locus_tag="ACHKAY_003295"
     CDS             6381..7157
                     /gene="yfaP"
                     /locus_tag="ACHKAY_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO5701921.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7432..10281
                     /gene="rcsC"
                     /locus_tag="ACHKAY_003296"
     CDS             7432..10281
                     /gene="rcsC"
                     /locus_tag="ACHKAY_003296"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO5701922.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10367..11017)
                     /gene="rcsB"
                     /locus_tag="ACHKAY_003297"
     CDS             complement(10367..11017)
                     /gene="rcsB"
                     /locus_tag="ACHKAY_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO5701923.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11034..13706)
                     /gene="rcsD"
                     /locus_tag="ACHKAY_003298"
     CDS             complement(11034..13706)
                     /gene="rcsD"
                     /locus_tag="ACHKAY_003298"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO5701924.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14445..15536
                     /gene="ompC"
                     /locus_tag="ACHKAY_003299"
     CDS             14445..15536
                     /gene="ompC"
                     /locus_tag="ACHKAY_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO5701925.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            15648..16703
                     /gene="apbE"
                     /locus_tag="ACHKAY_003300"
     CDS             15648..16703
                     /gene="apbE"
                     /locus_tag="ACHKAY_003300"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO5701926.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            16777..17841
                     /gene="ada"
                     /locus_tag="ACHKAY_003301"
     CDS             16777..17841
                     /gene="ada"
                     /locus_tag="ACHKAY_003301"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO5701927.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            17841..18491
                     /gene="alkB"
                     /locus_tag="ACHKAY_003302"
     CDS             17841..18491
                     /gene="alkB"
                     /locus_tag="ACHKAY_003302"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO5701928.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18567..20210
                     /gene="yojI"
                     /locus_tag="ACHKAY_003303"
     CDS             18567..20210
                     /gene="yojI"
                     /locus_tag="ACHKAY_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO5701929.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4890 a         4990 c         5445 g         4963 t
ORIGIN      
        1 actctaaatg tagtgttttg tcgcgttttg cagtatgtta tttaatctat tatatgaata
       61 acataatgat ttttaatgta tttttgtaat ttactctcct tctgacatgg aaggacaccc
      121 gatggatacc caacgattcc aatcccaatt tcattggcat ttatcgttta aattttctgg
      181 cgcgatagca gcgtgcttgt ccctgtctct tgtgggaaca gggcttgcta atgctgatga
      241 ttcgcttcct tccagtaact atgcgccgcc cgccggggga acattctttt tgcttgctga
      301 cagcagtttt agcagcagtg aagaggcgaa agtgcgactg gaagcgccgg ggcgtgatta
      361 tcggcgctat cagatggaag agtacggcgg cgtggacgtt cgcctgtatc gtattcctga
      421 cccgatggca tttttgcgcc agcagaaaaa cctgcatcgc attgtggtgc aaccgcaata
      481 tctgggcgac gggctgaaca atacgctgac ctggctgtgg gataactggt acggcaaatc
      541 tcgccgcgta atgcagcgta ctttctcttc tcagtcacgg cagaatgtga ctcaggcatt
      601 acccgaatta catctcggca atgccattat taaaccttcc cgttatgtac agaacaacca
      661 gttttccccg ctgaaaaaat atcccttggt ggaacagttc cgttatccac tatggcaggc
      721 taaaccggtc gagccgcagc aaggggtaaa actggaaggc gcatccagca atttcatctc
      781 gccgcagccg ggtaacattt atattcctct cggccaacaa gagccgggac tgtacctcgt
      841 cgaggcgatg gttggtgggt atcgggcgac gacggtggtg tttgtttccg ataccgtggc
      901 gcttagcaaa gtgtcaggca acgagcttct ggtgtggacc gcgggtaaaa aacagggtga
      961 agcgaagccc ggctcagaga tcttgtggac tgacggtctt ggcgtgatga cccgcggtgt
     1021 gaccgatgac agcggtacct tgcagttaca acatatatcg ccagaacgtt catacattct
     1081 gggtaaggat gctgaaggcg gtgtttttgt ctccgagaac ttcttctacg aaagcgaaat
     1141 ctacaacacc cgcttgtata tttttaccga tcgcccgcta tatcgcgcag gcgatcgtgt
     1201 cgatgttaaa gtgatgggcc gcgagttcca cgatccgttg cattcatccc ccatcgtcag
     1261 cgccccggcg aagctttcgg tgctggacgc taacggcagt ctgttgcaaa ccgtcaatgt
     1321 cacgctggat gcgcgcaatg gcgggcaggg aagtttccgc ctgccagaaa atgccgtagc
     1381 cggaggttat gagttacgtc ttgcttaccg caatcaggtc tatagcagca gttttcgcgt
     1441 ggcaaactac atcaagccac atttcgagat tggtttagct ctcgacaaaa aagagttcaa
     1501 aactggcgaa gcggtcagcg gcaaactgca actgctttac ccggatggtg agccggtaaa
     1561 agatgcccgc gtgcagttaa gtttgcgcgc tcagcaatta tcaatggtcg gtaacgattt
     1621 gcgttatgcc ggacgttttc ccgtgtcgct ggaaggcagc gaaacggtgt ccgacgccag
     1681 cggtcatgtg gcgttaaatc tccccgccgc cgataaaccg agccgctatt tgttaaccgt
     1741 ctccgccagt gacggcgcgg cgtatcgcgt caccaccacc aaagagatcc tcattgaacg
     1801 cggcctggcg cattactctt taagtaccgc cgcacaatac agtaatagcg gcgagtcggt
     1861 tgtgttccgt tatgccgcgc tggaatcttc aaaacaggtt cctgttacgt atgaatggtt
     1921 gcgtctcgaa gaccgcacga gccatagcgg agagctaccg tcaggcggca aatcctttac
     1981 cgtcaatttc gctaaacctg gcaactacaa tctgacatta cgcgataaag acggcttaat
     2041 tctcgctggg ttaagtcatg ccgtcagcgg taagggcagc acggcgcata ctggtacggt
     2101 agatatcgtg gcggataaaa cgctgtacca gccaggcgaa actgcgaaga tgctgattac
     2161 ctttccggag ccaattgatg aagcattatt gacgctggaa cgcgatcgcg tggaacagca
     2221 gtcgctgctt tcgcatccgg caaactggct gacgttacaa cgtttaaacg atacccagta
     2281 tgaagcccgg gttccagtga gcaattcctt tgcgcctaac atcacttttt cggtgctgta
     2341 tacccgtaat ggtcagtaca gttttcagaa cgccgggatc aaagttgccg ttcctcagct
     2401 tgatatccgg gtgaaaacgg acaaaaccca ttaccagcct ggtgaactgg tcaatgtcga
     2461 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
     2521 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
     2581 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc
     2641 gctctccagc gagccggttg cgcctggcgc aactaaccgc agcgagcggc gagtaaaaat
     2701 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
     2761 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
     2821 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
     2881 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
     2941 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
     3001 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
     3061 ttcgctgacg cagaacattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
     3121 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
     3181 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
     3241 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
     3301 tgcgttagtc gatggaccat ggggtggcgt gatcaacacc ggtagccgtc tgatcccgct
     3361 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
     3421 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     3481 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     3541 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     3601 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     3661 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg atgaagctat
     3721 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     3781 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     3841 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     3901 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     3961 tagcggtggt gacgctaccc agacggccgc tattttaagt ggtttgaccg ctgagcaatc
     4021 cactattgag cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga caatgcctcc
     4081 agttgttttg cctgcgcctg cgggcgcatg ggctaaacat aagttaactg gagggggcga
     4141 agactggcgt tgggttggtc agggcgtgcc ggacattctc tcttttggtg acgaattatc
     4201 gccgcaaaat gtgcaggtcc gctggcgtga gccggcaaaa atggctcaac aaagtaacat
     4261 tccggtgacc gttgaacgcc agttgtatcg gcttatccct ggtgaagaag agatgagctt
     4321 tattctgcaa ccggtgacca gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat
     4381 tacgcttacc agcgagcagg atgcagttct gcgctatggt caggtggaag taccgctccc
     4441 gccgggagcc gacgttgagc gcacaacatg gggcatttca gtcaataaac ccaacgccgc
     4501 gaaacagcag gggcaattgc tggaaaaagc gcgtaatgaa atgggcgaac tggcctatat
     4561 ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc gcttctcgca
     4621 aaaagggcaa ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac ctgcgcagca
     4681 aagtgttgcg gcagggagtg aatggaccgg gatgcaggtg aaataagtga actggcgcag
     4741 aatcgtctgg ctattggcgt tggttactct gccaacgctt gcggaggaga cgccgttaca
     4801 actggtgctg cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat caggcgtgac
     4861 caaagtctca gcattacctg actcgctcac cacgccgtta gggagtttgt ggaaactgta
     4921 tgtttacgcc tggctggagg atacccatca accggagcag ccctaccagt gtcgcggaaa
     4981 ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg ataccgcgct
     5041 ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg cagatgtgtg
     5101 ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta caacgctgaa
     5161 gccagaaacc tcggtaacgg ttaaatcgct gctcgattca ttagcgacgt tacctgcgca
     5221 aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa tcggtgtcgc
     5281 atccatgctc ggtagtcggg tacgggtgaa aacctggagc tggtttgccg atgataaaca
     5341 ggaaatccgc cagggtggtt ttgctggctg gttaaccgat ggtacaccgc tgtgggttac
     5401 tggcagtggc accagcaaaa cggtactaac ccgttatgcg acggtgctta atcgggtttt
     5461 gccagtgccg acgcaggttg ccagtgggca atgtgtagag gtggagctgt ttgcccgcta
     5521 tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaacccg gcgtacttaa
     5581 tgggcgctat cgtgtgacgt ttactaatgg taaccacatt acgtttgtca gccacggtga
     5641 gacgacctta ctgagcgaaa agggcaaact gaaattgcag tcgcatctcg atcgtgaaga
     5701 gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccccctgagg cagcgaaagc
     5761 catgaccgtg gcgattcgca cttttttaca gcaaaacgcc aatcgggaag gcgattgcct
     5821 gaccatcccc gacagcagcg ccacgcagcg tgtttctgct tcgccagcga ccacgggggc
     5881 gcgaacgatg accgcctgga cgcaggatct catctatgca ggcgatccgg ttcattatca
     5941 tggcagtcga gctaccgaag ggacactttc ctggcggcaa gccacggccc aggcggggca
     6001 gggagagcgt tacgaccaga tactcgcgtt tgcttatccc gacaacagcc ttagccgctg
     6061 gggagcgcca cgctcaacct gccagttatt acccaaagcg aaagcctggc tggcgaaaaa
     6121 aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac cagacgtgtt
     6181 tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga aacggctgtt
     6241 tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt atctgcacct
     6301 ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc tgacccgaca
     6361 attattaatg gactaatgct atgcgaaaga tttttcttcc gttgttactg gtggcacttt
     6421 cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg ctctctggct
     6481 ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac ccggcgtcgt
     6541 ccgtcaacat ggcagatgat caaaatatct cagcgcagat ccgcggcaaa ataaagaatt
     6601 atgctgcggc gggtaaagtt cagcagggtc ggctggtggt caacggtgcc agtatgccgc
     6661 agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa ggcagcaaca
     6721 gcgtgcaggt catcagcccg gatgggcaaa gccggcaaaa aatgcagttt tactcaacgc
     6781 cgggtacagg aacgattcgt gcacgtttac ggctggttct ctcgtgggat acggacaata
     6841 ccgatctcga tctccatgtt gtcacgcccg atggcgaaca cgcctggtac ggtaacaccg
     6901 tgctgaaaaa cagtggtgca ctggatatgg atgtcacgac ggggtacgga cccgagattt
     6961 tcgccatgcc agcaccagtt cacgggcgct atcaggtgta tatcaactac tatggtgggc
     7021 gcagcgaaac ggaattgacg accgcccaac tgacgctgat caccgatgaa ggatcggtca
     7081 atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg acgctggtga
     7141 aaagttttga ctggtgatcc atcccttcac acaacctggc tctctctttt ttcagataag
     7201 actgctgggc gatctgccga cgccaggata taaccattaa caagcgtcgg cagattggtc
     7261 atttcttatt tttcgtactg agaactgtcc atctactccg gctttcctgg ttaaggcaat
     7321 ggtttctaag cgatacgcat tattgcaatg gtgacagaac attgcggtaa cacgctttta
     7381 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
     7441 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
     7501 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
     7561 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
     7621 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
     7681 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
     7741 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
     7801 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctcaac
     7861 cgggtatttt taatcggcag tgataacctc tgcatggcca atttcggtct gcgtgatatg
     7921 ccagtggaac gcgataccgc gttgaaagcg ttgcatgaac gcatcaataa atatcgaaat
     7981 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
     8041 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
     8101 ggtgtcgagc agaccatccg gatggaaaac tttttcttac cgggtacgtt gccgatgggg
     8161 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
     8221 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
     8281 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
     8341 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
     8401 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
     8461 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
     8521 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
     8581 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
     8641 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
     8701 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
     8761 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
     8821 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
     8881 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
     8941 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
     9001 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
     9061 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
     9121 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
     9181 atgcgtttac agcaggtcat ctccaacctg ctgagtaacg ccataaaatt caccgatacc
     9241 ggctgtatag ttttgcatgt tcgcgctgat ggcgattatc tctctatccg tgttcgcgat
     9301 accggcgtgg gcattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
     9361 ggaacgggcg tacagcgtaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
     9421 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
     9481 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
     9541 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
     9601 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
     9661 gaagatgtgt taatcactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
     9721 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
     9781 agtgtggctg ctccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
     9841 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataacgac
     9901 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
     9961 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta
    10021 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    10081 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    10141 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    10201 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    10261 aggaaatcgc gggaatcgta ggacggataa ggcgtttacg ccgcatccgg cattcagtgc
    10321 aaatgccaga taagacacta acgcgtctta tctggcctac aggtgattag tctttatctg
    10381 ccggacttaa cgtcacagaa gagagataat tcagcagggc gatatcgttc tcgacaccca
    10441 gcttcatcat cgcagatttc ttctggctac tgatggtttt aatactgcgg ttcagctttt
    10501 tagcgatctc ggtcaccagg aagccttccg caaacaggcg cagaacttca ctctcttttg
    10561 gcgagagacg cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc
    10621 tttccggggt aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg
    10681 gtgcaccttg tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg
    10741 ccgggttgtt gttcatggtc agaacaataa tcgacaggct tgggaaatgg cgcttgatgt
    10801 acttgattaa ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca
    10861 acacatgcgc atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc
    10921 cgacaacatt cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg
    10981 gatggtcatc ggcaataatt acgttcatat tgttcatgta ataggctacc ttgctacagc
    11041 aagctcttga cataactgtc aatgtcgctg atgtattttt ctattcctgg aacatccttc
    11101 tcacgaatca gatgttccag cgtttcacat aactgcttgc cgggtaccag atttagcatg
    11161 gcaaatacgc ctttaagacg atgagccgtt tgggctaacg cagcaaagtc actggttgct
    11221 gcttcagtat acagcctctt aacatcatcc ggtactgtgt ctacaaagag cgcataatag
    11281 ccgctggcat ggagttgcgc attttcatct ccgcccagag gcgattctgt cacctcttcc
    11341 tgcgccagtt gcacttcaat taattgtaag accgcttcct gcatagcgtt gctcatattg
    11401 aagttgacgc acaattgacc aggcccaatt tcccgtacgc cagactcatc atcgcttaaa
    11461 agcaagccag aggcagtaag attagacgga ttatccgtta aaaagatatc ataatcttga
    11521 ctaattaatc tttcatcggg tgtgatacag gttgcacccc aattttctaa ctggcgagtg
    11581 acaatattcc gaatttctgc cgaagtaaca tccaccatta cgcagacatc atccagtaaa
    11641 cgctcttctt cctcttcaac ttccgggtca gcggcgagca ttttgatatg cacagagtag
    11701 cgtgtaccaa gcccatcccg cgttttgatg tttaaatgac cgcccagttt acgcgccagt
    11761 tgatcgctca gccagaatgc cagcgggtcc gccttgccat agcgatcgtt ttgggtctgg
    11821 ttgataaacg ggaagtgcaa attatccatt tcatgaatac ttacgccttc tccggtgtcc
    11881 agaatgcgga atgtcaggcg gtcttcagag gactcatcct gatcaacctc aagggtgatt
    11941 tttcccaatt gcgttgaggt cacggcatat tgcatcagta gcagcaaaat acgtcgtaag
    12001 gcatcgcgat cgccgcggcg catatcgtgt gctttcagat gattgttaat cagcagttgc
    12061 agacctttac gcttgatggc aggcaacact gaaggcacaa cttcatcaat taaatcctgc
    12121 acggagaaca gcaccgtctc acttttccag ctatcatccg caagcatgtt cgctaactgt
    12181 atttcatcaa ccagccgcac cagcacatct gcctgattcg ccagttgttt gctttccggg
    12241 gcgttgagtt tagccgcgct ctccgccagg gactgtgcag gttctttcag cgcatcgcca
    12301 atgtttttca taaaggtcat ccggccctgc tggtttttct catacagacg ctgcgcctgc
    12361 ttgagtttct tgtttaccag cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg
    12421 cgcggcgcga cctggctgcg gaacatgcgg atctcataca gctcgttatt gatcgtcgcc
    12481 tgaataatcc cctgatgctg ttccgccatg gtggtgatgt tttgcagatt caaatgcggc
    12541 agcaaatgat cggcaatttt gttacttatg acagtgcggt tcgattcctg atcgtgaacc
    12601 agcaggccga gcggcagcag tgagactatc tcttcattaa ttgcccgtaa aatgcgcaat
    12661 tcgttattga ccgccgtgct gggtagactt tctgtactgc ggctggagaa atggcggaat
    12721 gtggtatagc caaataacgc cagcgccagc aaaccgatgt tcagcagcag tggcagcaga
    12781 atgttttgca acgtatccag caataaggtg ccataaggaa cctgccagac caggcgcata
    12841 tcggtagagt tgagtgccga ggagatttca atcttcgtac tgttaaagtg gatactgacg
    12901 ctatccgtcc cttctttctc attatcattg tttcccgttg ccgtcgcgtc tggctcaagg
    12961 cggaaactgt ccagcggcat acccggtggg atcaaatcat taatcggcag atcaaaagcc
    13021 acgaccgttg ccagatgtcc tggctggtta aaggtagtac gcaaggtaaa gtaatgaccg
    13081 ttctgccagg ccaggcggcg catgttagaa aagctttcgc gttcatcgag ggcgttggcc
    13141 tgttgcaaca tctctgcacg acgtgaatca acaatgtcgc tgacggtcga ttctttaaat
    13201 ccggaggtga gatctttaag gggtagggtt gagatcagca ccagactgtt atcctgacca
    13261 ttcaggtaat acatcgacca cggtacattt tctgcgcccc acaatgtatc cagataagtg
    13321 gacatccgct gagtcatctc aagcgttgag ttgtcgtgag agccaaagat taacgcttcc
    13381 gttttgcggc gtggtttctc cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa
    13441 ccttcgccgg aggaggggga ggtcgtcgcg gcaatgttgt cgtagatctg ccaggtcacg
    13501 taacgccagg tatcgacgcg cttttggata gcgtgggtaa tgtcgacaat ctggtaactt
    13561 ttatctttca gccatgcgtt aacggcgctt tgtaccatta cacccatcgt caccagtaac
    13621 acaatgatca acagtaaaaa gaagcgggta atgctccccg gtaggagtga aaagcgggtc
    13681 gtggccgttg tctctttctg acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg
    13741 atagggcagt ataaagggta cagtgtgaaa ttccagactc ttacctgatt gatgattctc
    13801 tattctcccg tgatatttat catcctgcat tgggggaaat agatgtacaa gcgccatttt
    13861 gctgtacaaa taacctacaa aaagcccaac gaaaataatt gcaaataaac aaagattgct
    13921 ggaaattatg ctgatgttaa ttatttgtga aatagttaac aagcgttata gtttttctgt
    13981 ggtagcaaag aataatgaaa agtgtgtaaa gaagggtaaa aaaaaccgaa tgcgaggcat
    14041 ccggttgaaa taggggtaaa cagacattca gaaatgaatg acggtaataa ataaagttaa
    14101 tgatgatagc gggagttatt ctagttgcga gtgaaggttt tgttttgaca ttcagtgctg
    14161 tcaaatactt aagaataagt tattgatttt aaccttgaat tattattgct tgatgttagg
    14221 tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac attttgaaac
    14281 atctatagcg ataaatgata catcttaaaa gttttagtat catattcgtg ttggattatt
    14341 ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc agtatgcagt
    14401 ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa gttaaagtac
    14461 tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct gaagtttaca
    14521 acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac tatttctctg
    14581 acaacaaaga tgtagatggc gaccagacct acatgcgtct tggcttcaaa ggtgaaactc
    14641 aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag ggcaacagcg
    14701 ctgaaaacga aaacaactcc tggacccgtg tggcattcgc aggtctgaaa ttccaggatg
    14761 taggttcttt cgactacggt cgtaactacg gcgttgttta cgacgtaact tcctggaccg
    14821 acgtactgcc agaattcggt ggcgacactt acggttctga caacttcatg cagcagcgtg
    14881 gtaacggttt cgcgacctac cgtaacactg acttcttcgg tctggttgac ggcctgaact
    14941 ttgctgttca gtaccagggc aaaaacggta gcgtaagcgg cgaaggcatg actaacaacg
    15001 gtcgtgaagc actgcgtcag aacggcgacg gcgttggcgg atctatcact tatgattacg
    15061 aaggcttcgg tatcggtgct gcagtttcca gctccaaacg tactgatgat cagaacagcc
    15121 cgctgtacat cggtaacggc gaccgtgctg aaacctacac tggtggtctg aaatacgacg
    15181 ctaacaacat ctacctggct gctcagtaca cccagaccta caacgcaact cgcgtaggtt
    15241 ccctgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac cagttcgact
    15301 ttggtctgcg tccgtccctg gcttacctgc agtctaaagg taaaaacctg ggtcgtggct
    15361 acgacgacga agatatcctg aaatatgttg atgttggtgc gacctactac ttcaacaaaa
    15421 acatgtccac ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg
    15481 acgctggcat caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc
    15541 gattgatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaacac
    15601 agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct
    15661 ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac
    15721 aaaccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaact atgggtacct
    15781 tctggcgtgc cagcatcccg ggcattgacg ccaaacgcag tgccgaactt aaagaaaaga
    15841 ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc
    15901 tgatgcgctt taacgattcg caaagtttat cgccgtggcc ggtaagtgaa gcgatggccg
    15961 atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg gatataaccg
    16021 tcgggccgct ggtgaatctg tggggctttg gcccggaaca acagccggtt caaattccga
    16081 gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatta
    16141 atcagtcgca tcagcaatat ctgcaaaaag acctaccgga tttatatgtc gatctctcca
    16201 ccgtcggtga aggttatgcg gcggatcacc tggcacgctt gatggagcag gaagggattt
    16261 cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc
    16321 tgccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggctgtgg
    16381 tggatatcaa cggtcatggt attagcacct ctggcagtta ccgtaactat tacgaactgg
    16441 acggcaaacg tctttctcat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc
    16501 tggtatccgt gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct
    16561 tgatggtact cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt
    16621 atatgatcac caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct
    16681 tccttgtcag cgaaaaaaat taaagcgcaa gattgctggt ttttgcgtga tggtgaccgg
    16741 gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact
    16801 gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacag cgaattcgtt
    16861 ttcgccgtgc gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg
    16921 cgggaaaatg tctccttcta cgcaaatgcc tgcgaggcac tcgccgccgg atttcgcccc
    16981 tgcaaacgct gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc
    17041 cacgcgtgtc gactgctgga acaggaaacg cctgtaacgc tggaagcctt agccgaccag
    17101 gtggcgatga gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct
    17161 aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag
    17221 agcgtgacga cgtctattct taacgccgga ttccccgaca gcagcagtta ctatcgcaaa
    17281 gctgacgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg
    17341 gtgcgttacg cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg
    17401 gggatttgcg cgatattgct gggcgatgat gacgcgacac taatcagcga gttgcagcag
    17461 atgtttcccg ctgccgacaa cgcgcctgcc gatctgatgt ttcagcaaca tgtgcgtgaa
    17521 gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga cattcgcggc
    17581 actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc
    17641 agttatcagc aactggctaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc
    17701 gcctgtgccg ccaacaagct ggctatcata ataccctgtc atcgggtggt ccgtggtgat
    17761 ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct gcgccgcgaa
    17821 gctgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagagc
    17881 cactggcggc tggagcggta attttgcacc gttttgcttt taacgctgcg gagcaactga
    17941 tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc acccccgggg
    18001 gatataccat gtcggtggcg atgaccaact gtgggcatct gggctggacg acccatcggc
    18061 aaggttatct ttattcgccc attgatccgc aaacaaataa accgtggccc gccatgccac
    18121 aaagctttca tgatttatgt caacgtgcgg ctacggcggc gggctatccg gatttccagc
    18181 cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata
    18241 aagacgaacc ggatctgcgc gcgccgattg tttctgtttc tcttggctta cccgcgattt
    18301 ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg
    18361 atgtggtggt atggggcggt gaatcgcggc tgttttatca cggcattcaa ccgctgaaag
    18421 cggggtttca tccactcacc ggcgactgcc gctacaacct gacattccgt caggcaggta
    18481 aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc
    18541 tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc
    18601 gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct
    18661 taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtattgc
    18721 cggagtttct gggattattg ctgctgttga tggcagtcac tctcggatcg caactggcgc
    18781 tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc
    18841 tggataccaa cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg gcgggattaa
    18901 ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct tccggaactg gtgcagggga
    18961 tcattctcac tatcggttcc gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg
    19021 taacggctat ctggatggcg atcaccatct ggggtggttt tgtactggtg gcgcgggtgt
    19081 acaaacatat ggcgaccctg cgtgaaaccg aagacaggct gtatacggat tttcaaactg
    19141 tactcgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca
    19201 acctctacat tcctgatgcg caagagtatc gccaccatat tatccgcgca gacaccttcc
    19261 atcttagcgc cgtgaactgg tcaaacatca tgatgttggg cgcaatcggc ctggtgttct
    19321 ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat tcgttgacgc
    19381 ttttattcct gcgtacgccg ctgctttcgg cggttggcgc attgccgacg ctgctgacgg
    19441 cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc
    19501 cgcgcccgca ggcgtttccc aactggcaaa cgctggagct gcgtaacgtg acgtttgctt
    19561 atcaggataa cgcgttttcc gttggtccga ttaatctcac catcaaacgt ggcgagctgc
    19621 tgtttctgat tggcggcaac ggtagcggaa aatcgacgct ggcgatgttg ttgacgggct
    19681 tgtatcagcc acaaagcggc gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac
    19741 cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac
    19801 tgctggggcc ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc
    19861 tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa
    19921 aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc
    19981 tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgt
    20041 tgctgccgct gatgcaggag atgggtcaaa ctattttcgc catcagtcat gatgatcatt
    20101 actttatcca cgccgaccgc ctgctggaaa tgcgcaacgg gcaacttagc gagctgacgg
    20161 gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg
    20221 ctgacctggc ggactgctgc cgtcaggtca atatgctaaa aatcccttca tttctaataa
    20281 ccctataa
//
LOCUS       DAZFKM010000089        20186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFKM010000089 DAZFKM010000000
VERSION     DAZFKM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..238
                     /gene="torI"
                     /locus_tag="ACHKAY_003304"
     CDS             38..238
                     /gene="torI"
                     /locus_tag="ACHKAY_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFO5701930.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(767..2014)
                     /locus_tag="ACHKAY_003305"
     CDS             complement(767..2014)
                     /locus_tag="ACHKAY_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO5701931.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2086..3000)
                     /locus_tag="ACHKAY_003306"
     CDS             complement(2086..3000)
                     /locus_tag="ACHKAY_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO5701932.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            3216..4649
                     /gene="cscA"
                     /locus_tag="ACHKAY_003307"
     CDS             3216..4649
                     /gene="cscA"
                     /locus_tag="ACHKAY_003307"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO5701933.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(4657..5652)
                     /locus_tag="ACHKAY_003308"
     CDS             complement(4657..5652)
                     /locus_tag="ACHKAY_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5701934.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            5895..6113
                     /locus_tag="ACHKAY_003309"
                     /pseudo
     CDS             5895..6113
                     /locus_tag="ACHKAY_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            6131..7459
                     /gene="dsdA"
                     /locus_tag="ACHKAY_003310"
     CDS             6131..7459
                     /gene="dsdA"
                     /locus_tag="ACHKAY_003310"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO5701935.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(7567..9105)
                     /gene="emrY"
                     /locus_tag="ACHKAY_003311"
     CDS             complement(7567..9105)
                     /gene="emrY"
                     /locus_tag="ACHKAY_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO5701936.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(9105..10268)
                     /gene="emrK"
                     /locus_tag="ACHKAY_003312"
     CDS             complement(9105..10268)
                     /gene="emrK"
                     /locus_tag="ACHKAY_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO5701937.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            10684..11298
                     /gene="evgA"
                     /locus_tag="ACHKAY_003313"
     CDS             10684..11298
                     /gene="evgA"
                     /locus_tag="ACHKAY_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO5701938.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            11303..14896
                     /gene="evgS"
                     /locus_tag="ACHKAY_003314"
     CDS             11303..14896
                     /gene="evgS"
                     /locus_tag="ACHKAY_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO5701939.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(14952..16097)
                     /gene="yfdE"
                     /locus_tag="ACHKAY_003315"
     CDS             complement(14952..16097)
                     /gene="yfdE"
                     /locus_tag="ACHKAY_003315"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO5701940.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(16171..17115)
                     /gene="yfdV"
                     /locus_tag="ACHKAY_003316"
     CDS             complement(16171..17115)
                     /gene="yfdV"
                     /locus_tag="ACHKAY_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO5701941.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(17185..18879)
                     /gene="oxc"
                     /locus_tag="ACHKAY_003317"
     CDS             complement(17185..18879)
                     /gene="oxc"
                     /locus_tag="ACHKAY_003317"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO5701942.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(18933..20183)
                     /gene="frc"
                     /locus_tag="ACHKAY_003318"
     CDS             complement(18933..20183)
                     /gene="frc"
                     /locus_tag="ACHKAY_003318"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO5701943.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         5851 a         4730 c         4342 g         5263 t
ORIGIN      
        1 tgatttgcag gttcgattcc ctattcggat agcactcatg caacacgaac tacaacctga
       61 ttcactggtt gatttgaaat tcatcatggc tgatactggc tttggtaaaa ccttcatcta
      121 cgaccggatt aagtccggcg acctgcctaa agccaaagtt atccacggtc gagcaagatg
      181 gttatatcgt gaccattgtg aattcaaaaa taagctctta agccgcgcta atgggtaaaa
      241 tagcgggtaa aatatttctc acatctaaaa aataccattc taatcaatcc cctgccactc
      301 taagtagatg tctgcagggg acaccagata cccttcaaac gaaatctacc ttcaccccgt
      361 aaaagatagg tttggcagca cacttgcctt atatctactc atttttactg caacaggttg
      421 aaatctcagc actgtcagaa agcgctgatg actaaacagc cctgggccgg gcgatgtaac
      481 catcacacag aatcctgata gcgaaatatg gcgtgactcg atacttcact ccgcaatgca
      541 ttccttgatg aattcgcagg accgtgatac acgggacagg tcgctgaatg acgacaatgt
      601 cctggaaatc agcgaaccgc gcatctgaag tacatttgag cgactgtacc agaacatgaa
      661 tgaggcgttt ggattaggtg attattagca gggctaagca ctttagtatt attattttcc
      721 ggttgaggga tagggagata tcgacaacaa ccggaaaagt ttacgtctat attgctgaag
      781 gtacaggcgt ttccataact atttgctcgc gttttttact caggaagaaa atgccaaata
      841 gcaacatcag gcagacaata cccgaaattg cgaagaaaac tgtctggtag cctgcgtggt
      901 caaagagtat cccagtcggc gttgaaagca gcacaatccc aagcgaactg gcaatttgaa
      961 aaccaatcag aaagatcgtc gacgacaggc gcttatcaaa atttgccacg ctgtatttga
     1021 agacggatat gacacaaagt ggaacctcaa tagcatgtaa cagcttcact aatgaaataa
     1081 tccaggggtt aacgaacagc gcgcaggaaa ggatacgcaa cgccataatc acaacaccga
     1141 taagtaatgc attttttggc cctacccgat tcacaaagaa aggaataatc gccatgcata
     1201 gcgcttcgag taccacctgg aatgagttga gataaccata caggcgcgtt cctacatcgt
     1261 gtgattcgaa taaacctgca taaaagacag gaaaaagttg ttgatcaaaa atgttataga
     1321 aagaccacgt ccccacaata aatatgacga aaacccagaa gtttcgatcc ttgaaaactg
     1381 cgataaaatc ctcttttttt acccctcccg catccgccgc tacgcactgg tgatccttat
     1441 ctttaaaaca catgttgatc atcataaata cagcgccaaa tagcgagacc aaccagaagt
     1501 tgatatgggg actgatacta aaaaatatgc cggcaaagaa cgcgccaata gcatagccaa
     1561 aagatcccca ggcgcgcgct gttccatatt cgaaatgaaa atttcgcgcc attttttcgg
     1621 tgaagctgtc aagcaaaccg catcccgcca gataccccag gccaaaaaag agcgccccca
     1681 gaattagacc tacagaaaaa ttgctttgca gtaacggttc ataaacgtaa atcataaacg
     1741 gtccggtcaa gaccaggatg aaactcatac accagatgag cggtttcttc agaccgagtt
     1801 tatcctgaac gatgccgtag aacatcataa atagaatgct ggtaaactgg ttgaccgaat
     1861 aaagtgtacc taattccgtc cctgtcaacc ctagatgtcc tttcagccaa atagcgtata
     1921 acgaccacca cagcgaccag gaaataaaaa agagaaatga gtaactggat gcaaaacgat
     1981 agtacgcatt tctgaatgga atattcagtg ccataattac ctacctgtcg ttaaaaaatt
     2041 cacgtcctat ttagagataa gagcgacttc gccgtttact tctcactatt ccagttcttg
     2101 tcgacatggc agcgctgtca ttgccccttt cgccgttact gcaagcgctc cgcaacgttg
     2161 agcgagatcg ataattcgtc gcatttctct ctcatctgta gataatcccg tagaggacag
     2221 acctgtgagt aacccggcaa cgaacgcatc tcccgccccc gtgctatcga cacaattcac
     2281 agacattcca gcaaaatggt gaacttgtcc tcgataacag accaccaccc cttctgcacc
     2341 tttagtcacc aacagcatgg cgatctcata ctcttttgcc agggcgcata tatcctgatc
     2401 gttctgtgtt tttccactga taagtcgcca ttcttcttcc gagagcttga cgacatccgc
     2461 cagttgtagc gcctgccgca aacacaagcg gagcaaatgc tcgtcttgcc atagatcttc
     2521 acgaatatta ggatcgaagc tgacaaaacc tccggcatgc cggatcgccg tcatcgcagt
     2581 aaatgcgctg gtacgcgaag gctcggcaga caacgcaatt gaacagagat gtaaccattc
     2641 gccatgtcgc cagcagggca agtctgtcgt ctctaaaaaa agatcggcac tggggcggac
     2701 cataaacgta aatgaacgtt ccccttgatc gttcagatcg acaagcaccg tggatgtccg
     2761 gtgccattca tcttgcttca gatacgtgat atcgactccc tcagttagca gcgttctttg
     2821 cattaacgcc ccaaaaggat catcaccgac ccgacctata aatccactta ttccgcctaa
     2881 tctggcgatt cccaccgcaa cgttagctgg cgcgccgcca ggacaaggca gtaggcgccc
     2941 gtctgattct ggcaagagat ctacgaccgc atcccctaaa acccatactt tggctgacat
     3001 ttttttccct taaattcatc tgagttacgc atagtgataa acctctttct cgcaaaatcg
     3061 tcatggattt actaaaacat gcatattcga tcacaaaacg tcatagttaa cgttaacatt
     3121 tgtgatattc atcgcattta tgaaagtaag ggactatatt tttataaaag ttaacgttaa
     3181 caattcacca aatttgctta accaggatga ttaaaatgac gcaatctcga ttgcatgcgg
     3241 cgcaaaacgc actagcaaaa cttcatgagc accggggtaa cactttctat ccccattttc
     3301 acctcgcgcc acctgccgga tggatgaacg atccaaacgg cctgatctgg tttaacgatc
     3361 gttatcacgc gttttatcaa catcacccga tgagcgaaca ctggggacca atgcactggg
     3421 gacatgccac cagcgacgat atgatccact ggcagcatga gcctattgcg ctagcgccag
     3481 gagacgataa tgacaaagac gggtgttttt caggtagtgc tgtcgatgac aatggtgtcc
     3541 tctcacttat ctacaccgga cacgtctggc tcgatggtgc aggtaatgac gatgcaattc
     3601 gcgaagtaca atgtctggct accagtcggg atggtattca tttcgagaaa cagggtgtga
     3661 tcctcactcc accagaagga atcatgcact tccgcgatcc taaagtgtgg cgtgaagccg
     3721 acacatggtg gatggtagtc ggggcgaaag atccaggcaa cacggggcag atcctgcttt
     3781 atcgcggcag ttcgttgcgt gaatggacct tcgatcgcgt actggcccac gctgatgcgg
     3841 gtgaaagcta tatgtgggaa tgtccggact ttttcagcct tggcgatcag cattatctga
     3901 tgttttcccc gcagggaatg aatgccgagg gatacagtta ccgaaatcgc tttcaaagtg
     3961 gcgtaatacc cggaatgtgg tcgccaggac gactttttgc acaatccggg cattttactg
     4021 aacttgataa cgggcatgac ttttatgcac cacaaagctt tttagcgaag gatggtcggc
     4081 gtattgttat cggctggatg gatatgtggg aatcgccaat gccctcaaaa cgtgaaggat
     4141 gggcaggctg catgacgctg gcgcgcgagc tatcagagag caatggcaaa cttctacaac
     4201 gcccggtaca cgaagctgag tcgttacgcc agcagcatca atctgtctct ccccgcacaa
     4261 tcagcaataa atatgttttg caggaaaacg cgcaagcagt tgagattcag ttgcagtggg
     4321 cgctgaagaa cagtgatgcc gaacattacg gattacagct cggcactgga atgcggctgt
     4381 atattgataa ccaatctgag cgacttgttt tgtggcggta ttacccacac gagaatttag
     4441 acggctaccg tagtattccc ctcccgcagc gtgacacgct cgccctaagg atatttatcg
     4501 atacatcatc cgtggaagta tttattaacg acggggaagc ggtgatgagt agtcgaatct
     4561 atccgcagcc agaagaacgg gaactgtcgc tttatgcctc ccacggagtg gctgtgctgc
     4621 aacatggagc actctggcta ctgggttaac ataatatcag gtggaacaac ggatcaacag
     4681 cgggcaaggg atccgcgtca ctcttccccc ttcacgacct tcaataatat gcaatgcagc
     4741 ttcccgcccg ataatgtcat gtggaagctg aattgtggtc agcggcggta aaaacagatg
     4801 cccgacgcca accagattat caaagcccat tacggcgaca tcctgcggga ttcgtacccc
     4861 cttcgccaga agaacctgat aagccacaaa ggctgcgcga tcgttaccac atatcagaac
     4921 atcaaaatct ggtttgcccg atttgaagtg gtcattgagt aaacttgcga gatcggtgta
     4981 gtgatcatca cctgttgcca tgtgaaattg tttcacctca gccagatctc gtccagcatc
     5041 acgccaggcc tgctcaaatc cctgccgacg ataccctgtt gccaacgcac tttccggtag
     5101 ccagaagcat aacggttgac gatagcccgc cgcgagcaaa tgctgtgttg attcatattg
     5161 tgcagtgtaa tcatcaggga tataactggg taacgctggg tcatccgcca cacagttcgc
     5221 caatacaata ttttcaccat acagagactc aggcagcgtg atatgtcgca gccccattgt
     5281 agtatagata atgccatccg gacggtgggc aagcagctga cgtgccgcgc gggcagcgtc
     5341 atcttcagaa aaaatattga ttaaaaaact attccagccg aactcgctgg cggtttgctc
     5401 aatggcaagc agaatatcaa cagagaaagg agtggtagcc gtgtcctgcg ccagcacggc
     5461 gagagtcgac ggcttacgtc cttgagcgcg catcttacgg gcggaaagat caggaacata
     5521 attcagggtc tggattgcct gcaatacgcg gtcacgcgtt gcaggacgca cagattctgc
     5581 attatgcatc acccgggaga ctgtcatcat cgacactccc gccaggcgtg cgacatcctt
     5641 taatgaagcc atacccaagc cgtttgccgt aaaacgggca ctgtagcaga aacagacgtc
     5701 actggcgaga tccaacgata ttctcaacgc cctatcaccc gacacggcaa tacaataaaa
     5761 aataacaata attgccggat aattgtactc aattccgcct ctgttctcgc caacgagtaa
     5821 ccatataggc aagtaagtaa aacatgccaa ctggcatcgc caacctggag aagaaaatat
     5881 aaatatggat cattgtgctg ccgctgtatc ccgacatcag cccggaaatt attgcgattg
     5941 ctatcggttc aggtgcaatt ggctgcacga tcgttacgga ctcgcttttc tggctagtga
     6001 agcaatattg cggcgctacg ctcaatgaaa catttaaata ctatacgaca gcgacattta
     6061 tcgcttcagt cgtcgctctg gcgggcacat tcctgctgtc atttatcatc taagcgcaaa
     6121 gagacgtact atggaaaacg ctaaaatgaa ctcgctcatc gcccagtatc cgttggtaaa
     6181 ggatctggtt gctcttaaag aaaccacctg gtttaatcct ggcacgacct cattggctga
     6241 aggtttacct tatgttggcc tgaccgaaca ggatgttcag gacgcccatg cgcgcttatc
     6301 ccgttttgca ccctatctgg caaaagcatt tcctgaaact gctgccactg gggggattat
     6361 tgaatcagaa ctggttgcca ttccagctat gcaaaaacgg ctggaaaaag aatatcagca
     6421 accgatcagc gggcaactgt tactgaaaaa agatagccat ttgcccattt ccggctccat
     6481 aaaagcacgc ggcgggattt atgaagtcct ggcacacgca gaaaaactgg ctctggaagc
     6541 ggggttgctg acgcttgatg atgactacag caaactgctt tctccggagt ttaaacagtt
     6601 ctttagccaa tacagcattg ctgtgggctc aaccggaaat ctggggttat caatcggcat
     6661 tatgagcgcc cgcattggct ttaagatgac agttcatatg tctgctgatg cccgggcatg
     6721 gaaaaaagcg aaactgcgca gccatggcgt tacggtcgtg gaatatgagc aagattatgg
     6781 tgttgccgtc gaggaaggac gtaaagcagc gcagtctgac ccgaactgtt tctttattga
     6841 tgacgaaaat tcccgcacgt tgttccttgg gtattccgtc gctggccagc gtcttaaagc
     6901 gcaatttgcc cagcaaggtc gtatcgtcga tgctgataac cctctgtttg tctatctgcc
     6961 gtgtggtgtt ggcggtggtc ctggtggcgt cgcattcgga cttaaactgg cgtttggcga
     7021 tcatgttcac tgcttttttg ccgaaccaac gcactcccct tgtatgttgt taggcgtcca
     7081 tacaggatta cacgatcaga tttctgttca ggatattggt atcgacaacc ttaccgcagc
     7141 ggatggcctt gcagttggtc gcgcatcagg ctttgtcggg cgggcaatgg agcgtctgct
     7201 ggatggcttc tataccctta gcgatcaaac catgtatgac atgcttggct ggctggcgca
     7261 ggaagaaggt attcgtcttg aaccttcagc actggcgggt atggccggac ctcagcgcgt
     7321 gtgtgcatca gtaagttacc aacagatgca cggtttcagc gcagaacaat tgcgtaatgc
     7381 cactcatctg gtgtgggcga cgggaggtgg aatggtgccg gaagaagaga tgaatcaata
     7441 tctggcaaaa ggccgttaat aacgtttcaa cgcagcatcg caatcctttc cctgggtgag
     7501 cgatgctgcc gatggcgcag acttaagatc cccgctctta cccgctataa ccccctcctt
     7561 ttatcatcac ccaacgcctt tcgctgtaaa cggcggtttc gcaaaccaaa ccaaaaccgt
     7621 aagtaagata aaagcaatag ccgccatacg gaaaatctca tttgcagaaa tagaaagtga
     7681 ctgctgggtt atttcattat taatttcatt aagaactcct gaaagcgaac catagtattt
     7741 atccataatt tgtgacgatg aattaaacac ggggttaaat tgatcgatgg ttgctgtcaa
     7801 ctgactatgg tgtaacgatt cgcgtcgtcc ccacagcgtc attgtcaacg acgtaccaac
     7861 tgatcctgac aaggtacgaa aaaaattact catactcgag gcattggcaa atttattatc
     7921 tggcaagcct gaaaacgaaa tcgttgttaa gggtaaaaag aaacaggcaa cggcgaatcc
     7981 ctggaaaaac tgtggcaaaa tgatgcctgt aaaatcaatc gttggcataa atgtcacaga
     8041 acgccagtaa tagcaaaccg catacatcaa aaaactaaat gtcactaaca accgcatgtc
     8101 tattttgttg ccataacgtc ctatcaaagg tgaaattaat agtggcatga tgccgatggg
     8161 cgcataagca agtccggccc atatcgcatt ataccccatc gtttcctgga gtaactgcgg
     8221 cataaggacg atcgctccag agtaaaataa atacgcgcat gtgatactca caataccaat
     8281 ggtgaagtta cgggacttaa acaaactgag atcaagaatc gggttctctg aggtcgactc
     8341 ccaaatgact aaagagatca gagaaataac tgatactact gttaatatta ttattgtact
     8401 cgagttgaac caatccagat cgcgcccttt atcaagcata atttgcaagc caccaacacc
     8461 gagcactaac agggtcagtc ctggtagatt cattttgacc ggtgaagttt cagtttctct
     8521 tcctttaagt aaggttaagc ataatgtcag gacgataatc cccataggga cattgattaa
     8581 aaatatccaa ccccagctaa agttatcgca aatataaccg cccaatatcg gcccacatat
     8641 cggagcgata atcacggtca ttgaccataa tgccagagca aatgttcttt tttctggtgg
     8701 ataattcctt aataataaac tctgtgacag tggaattaac ggccccgcca ttaatccctg
     8761 aacgactcta aaaaatatca gcacatcaag attggtcgat aaactacaca ttaaggaaga
     8821 cagcgaaaaa aaagtgactg aaagtaaaaa taaccttaat tcgcctattc tttgtgccag
     8881 cctgccggta acagggatcg caatggcatt tgctacgcca aacgaggtga taacccaggt
     8941 gccttcgtct gttgatgctc ccagaaagcc agatattgtc ggtattgcga cgttagaaat
     9001 agtggaatcc aacatttgca taaacgtcgc taatgacaat gcaatagtaa cgcaccataa
     9061 cgtcccaccg gttaatggtg ccggagtcga tttagtgatt gccattaaag ttgtccatta
     9121 tgcgaaataa tgttgctaat ttctttttct atcggactgg tatcgataac taaagcctta
     9181 ctggtataag ccggcatgga cgtcacggtt gaagccagat caggcatctc agcaatgtct
     9241 tcgttcttcg tatcaatagt tgcagtcatc gataaaccaa tgcgtaaggg gtgttccatg
     9301 agttcttttg gatcaagaga aacttcaacc ggtacacgct gaacgatttt gatccagttc
     9361 cctgtcgcat tttgtgcagg taataaggag aacgcattgc cggttcccat attgatccct
     9421 gtcacccgac catgaaacac aacattttca ccataaagat cgctgataat attgactgat
     9481 tgaccaatcc gtacatccgt gagttgtgtt tctttaaagt tggcattaac ccacatttga
     9541 cgtgccggta cgacagccat taacgattgt ccgggactca ctgtttcgcc gacctgaaca
     9601 ctccgctggg caatatagcc agtaaccgga ctccttatat ccgtacgttt aagcgccaac
     9661 caggcttctt tagttgcatc cgccgcttcc acgacttgtg gctgacggtt taatggtgtg
     9721 ttcattacta aagctttatt cgctttataa gcctggatag cggcattcag tgccgctttg
     9781 ctacttatta acgtatcttt ggtatgctcc agcgtttctt ttgaaataac cccctgcttc
     9841 gctaacggta ctcgacggtt ataatcttct aaagattgtt gatactgaat acgtgctgaa
     9901 gcgacttcgg cactgtattg tttatcctgt aagtacagtt tattcgtttg ccgaacaata
     9961 tttgccagat tatttttagc tttattgagt gcgatagtgg catcagtttt atccagtgaa
    10021 actaaaatgt caccttgtcg aacgtagttc gtatctttat gattaacgac agtgacacta
    10081 cctgagactt gtgcagaaat tggatctgca ttccccgtga cataggcgtc atctgtacta
    10141 atcatgtctt ctaattccat tgaccaatag gcataggcac ctgaaaacgc aataaataaa
    10201 actactgcca ataaagaaaa gtattttctt ctgttagaat gttttttatt tgaattaatc
    10261 tgttccacta ttatctctca tttctcatag atgaaattta tgaattgaat gaaaagagca
    10321 ttcattcttc atatcaaaca cagtgcatgt gttgcacatt atcccaggaa cgttggaggc
    10381 ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa accgtggctt ttgcaataca
    10441 attcttacgc ctgtaggatt agtgagaaga cttatagtgc taacttgaaa ctataaatca
    10501 tcggtataat ccctgatttt attgttgaca tttcatttat gccgactatt tatatggtat
    10561 acttgtcgaa ttatcttaaa ggaagctcag attttcttat ttttattgag aaaatgagat
    10621 gacgccttat gtctgtatta ctacagggag aagggagatg cttcattgca aagggaataa
    10681 tctatgaacg caataattat tgatgaccat cctcttgcta tcgcagcaat tcgtaattta
    10741 ttgatcaaaa acgatattga aatcttagcg gagttgactg aagggggaag cgccgttcag
    10801 cgggtggaaa cacttaagcc tgatatcgtc atcattgatg tcgatatccc cggagttaac
    10861 ggtatccagg tgttagaaac gctgaggaag cgccaatata gcggaattat tattattgtc
    10921 tccgctaaaa atgaccattt ttacgggaaa cattgtgctg atgctggcgc taatggattc
    10981 gtgagtaaaa aagaaggcat gaacaatatc attgcggcta ttgaagctgc aaaaaatggc
    11041 tactgctatt tccccttctc tctcaaccgg tttgttggaa gtttaacgtc cgaccagcaa
    11101 aaactcgact ccttatcgaa acaagaaatt agtgtcatgc ggtatattct tgatggcaag
    11161 gataataatg acattgctga aaaaatgttc atcagcaaca aaactgtcag cacttataaa
    11221 agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg atctttacac attcgcgcaa
    11281 cgtaacaaaa tcggctaatc acatgaagtt tttaccctat ttttttcttc tctgttgtgg
    11341 tctttggtcg accataagtt tcgcagacga agattacatc gaatatcgtg gcatcagtag
    11401 taacaaccgt gtcacacttg atccagtacg tctgagcaac aaggaattac gttggttagc
    11461 gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa acggctacct tgttgcatac
    11521 cgattcgcag caacaggttc gtggtattaa tgctgattat ttaaatcttt taaaaagagc
    11581 gttaaatatc aaattaacac tccgggaata cgccgatcat caaaaagcaa tggacgcgct
    11641 tgcagaaggt gaagtcgata tagtgttatc acatttagtt acttcgccgc ctcttaatag
    11701 tgacattgct gcaaccaaac cattgataat tacctttccg gcgctggtaa ccacccttca
    11761 cgactcaatg cgaccgctta cctcaccaaa accagtaaat attgctcggg tagcaaatta
    11821 ccccccagac gaggtaattc atcaatcgtt tccaaaagca acaattatct cttttacaaa
    11881 tttatatcag gcattagcat ccgtatcagc cggacagaat gattacttta ttggtagtaa
    11941 catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg tagtgaaata
    12001 ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatccg tcattcttaa
    12061 tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg aagtttcaca
    12121 aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac tcactgaaca
    12181 tgaaaaacag tggattaagc aacatcccga tttaaaggtg ctggaaaatc cttactctcc
    12241 tccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg atattcttaa
    12301 tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac acaatatcca
    12361 tgccggaaca cagcttaacc ccggtggatg ggatataata cctggcgcta tttatagtga
    12421 agatcgagaa aataatgttt catttgcaga agctttcata acaacgccat acgtttttgt
    12481 catgcaaaaa gcacctgaca gtgaacaaac attaaaaaaa ggaatgaaag ttgccattcc
    12541 atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg aatggataca
    12601 ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg atgctctggt
    12661 cgcgacacag ttaaattcgc gttacatgat cgatcattac tatcctaatg aactttatca
    12721 ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc gcggagaacc
    12781 ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg aagttctgcg
    12841 cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg acctatatag
    12901 cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta gccttttatg
    12961 gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg atttagaaaa
    13021 ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt atgttgtaaa
    13081 ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca ctgcggatta
    13141 ctacaaaaat gcaatgttac cattagaaaa cagtgactca ccctttaaag atgttttttc
    13201 taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca cacaggtatt
    13261 tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat tatgcaatct
    13321 tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg aaacgcgtga
    13381 tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg tagcaaaaag
    13441 tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta ttatgggctt
    13501 cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg cgatttcact
    13561 tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg atgtcgacaa
    13621 aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta ctttagtcca
    13681 gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat taagttgcag
    13741 cagtacattt cctgatcatt acctggttaa gatcgaccct caggcgttta agcaggtctt
    13801 atcaaattta ctgagcaatg ctctcaaatt taccaccgag ggggcagtaa aaattacgac
    13861 ctccctggtt cacattgatg acaaccacgc tgttatcaaa atgacgatta tggattctgg
    13921 aagtggatta tcgcaggaag aacaacaaca actctttaaa cgctacagcc aaacaagtgc
    13981 aggtcgccag caaacaggat ctggcttagg cttaatgatc tgcaaagaat taattaaaaa
    14041 tatgcagggc gatttgtcat tagaaagtca tccaggcata ggaacaacat ttacgatcac
    14101 aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag aacaccccat
    14161 cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca acaggctatt
    14221 actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg atggtgtgca
    14281 agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg ttaatatgcc
    14341 gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt ccttacccat
    14401 ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa gttgcggcat
    14461 gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt taagtcagtt
    14521 acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag ccctgaaaaa
    14581 taatacggcg aacgatctac aactgatgca ggagattctc atgactttcc agcatgaaac
    14641 gcataaagat ctacccgctg cgtttcaagc actagaagct ggcgataaca gaactttcca
    14701 tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa agttgattaa
    14761 tattagccat cagttagaaa taacacctgt ttcagatgac agtaagcctg aaattcttca
    14821 gttgctgaac tctgtaaaag aacacattgc agagctggac caggagattg ctgttttctg
    14881 tcagaaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct atttaccagc
    14941 acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg gtcgagcgtt
    15001 gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg attacccggc
    15061 atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg caatttaatg
    15121 gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat tctggctaac
    15181 caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag aatagcctgg
    15241 ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc cgtaagctcc
    15301 agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt aatcggctta
    15361 tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc cagacgttgt
    15421 ggtgacttcc ctgtcgcgat atatgccatc agaccatgct ccagaaaact cagcgtggca
    15481 tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc ataaagtgca
    15541 ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga tgtaccaacg
    15601 cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat tgcctgaatg
    15661 atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc tgacgatgaa
    15721 gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc cagtttttcc
    15781 attgtacctg ggcgaaaatt ctcagctaat acatcagctt gtttgagcat atttataaat
    15841 atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc atgattaata
    15901 aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt atcatcacca
    15961 tgacccggcg gctcaacttt aattaccctt gcgcccatat tacaaagaag ttgagttccg
    16021 aaaggtccat taaggacatg tgtcatatcg ataactaata agccttcaaa cggccctttg
    16081 ctttcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa agacacttcg
    16141 gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga cataaatcca
    16201 caagggagcc gtgacgacaa agcccagtac gctcacagcc aatgacgctg taccagtacg
    16261 ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg gtaatgcgcc
    16321 tgccagtacc atcatctgca gatgttcgct gttcaaatga catgccatac caacgagaag
    16381 cagtgccagt ggcatcagaa tcagcttcag gaaggtgtta taagcaattt cggcactgaa
    16441 ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc ctgagttagc
    16501 cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc caaccaacac
    16561 caggatcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag aaattaatgc
    16621 gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca aatacagacc
    16681 aataggaata gtaattgcgt taacaataat agaaataatt gctaccacta aacctgttga
    16741 tacggaatca ccataaatag gatcgagaac tgcaaacccc aggaatccaa tggtaggtga
    16801 acccgcaatt aatgcacata cagctgcttc tgcatgggta cgtttaaaaa atttgtagca
    16861 accaaaccag gagaaaaaga aacatccgac aataaccaca agtgatacaa gggtcagacg
    16921 agtgtccgca aaaatcattt ccctgtttgc ccgggtaata gaaacaaata gagccgcagg
    16981 aagagcgtag tttaatacca gtttattaaa tgcgcgggct tgatcttctg aaaaagtttc
    17041 tcgtctgccg ctaaaataac ccaacagcat gattacgata ataggcaata aatcgccaat
    17101 aaaaaatgtt agcataatag tatttccgat aagggtcccc tcaccatcgc ataatgagtt
    17161 ggcaagagga gacgatgtca gtatttaatt accagcgact tgttttgggt taagtttggt
    17221 aatatggcca ctttcagttc ctgctgcagg gtcgatgacc acattaataa tggtcggttt
    17281 gcgcgactga ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg tgacgttata
    17341 gccaacgcca cgaaacgcat ccattaattt gtcatacctt gcatggtgca acagatccgt
    17401 tggtgatggt gcaccagcgc cactgagatc aacaccgtct cctctgtaga tgccgccatt
    17461 attaaaaata acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa tttccatccc
    17521 actgaaacca aaagcactat caccttcaat ggcgacaacc ggagaaccag aagtcacgct
    17581 agcaccgatg gcatagccca taccgatgcc catgacaccc caggtaccac aatccagacg
    17641 acgacgtggt ttatacatat caataatatt tcgtgcatta tccagggtat ttgcaccttc
    17701 attaactaaa taaatatcct ggttctcgcg caatacgtcg cgcacagcac ttaatgcatt
    17761 aaagtaattt aatggttgag tatctgtact taatttttca tgcatttttt gtgcattttg
    17821 ctgcttgtgg atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg tgttttgttt
    17881 cagttctgcc agcatacctt gcatactgga tgcaatatca ccaacgactg gcacagcaat
    17941 ggggcggttg ctgtcaattt cctgcggttc aatatccagt tgaataaact gtgtatctgc
    18001 cgcccatcct tttttaccgt gtgccaataa ccaattcagt cgtgcaccaa caagcatgac
    18061 aacgtcagca tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg gatgcgtatc
    18121 ttcaaggatc cctttcgcca tagacattgg caggaatgga atctgagtac tttcaataaa
    18181 ctcacgaagc tgttcatcag cttgtgaata cgccgcgcct ttgccaagga taattaatgg
    18241 ccgttcagct tttgctaaaa gcgaaattgc gctagtgact gacttcgggc atggcaataa
    18301 tgctggcgac ggattttcaa ctttaacaat cgtggttaac gcttcgtctt tttccatcgt
    18361 cgcggccagg acatttgctg gcaaatcaag ataaactccg ccagggcgac ccgatacaga
    18421 gacccggata gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa cgcgaaatgc
    18481 tgctttggca tacggttttg ccgcattcat ttggtccagc tcttcataat caccttgctg
    18541 taggtcgacg atcgcgcggt cgctggagcc gctaatcatg atcatcggaa aaccatttac
    18601 cgttgcgttg gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa ctgtcaggca
    18661 gatccccggt ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg ccgactgctc
    18721 atgacgaaaa ccaatataac gaatgccttc cgcctgggca tggcgtgcca tatccgtcac
    18781 aggaatacct acaacaccat aaatagtgtc aatattattc tgttttaatg cttcaacgat
    18841 gatatgcata ccatctgtca tttgaagttg atctgacatg attaataacc tcgaaatttt
    18901 tataaattgg gagccagaag cccccgttaa tatcagatgg cgtggttttg cttcattgca
    18961 gcaatttcat cgtcgctata acccagctcc tgcaatacag cagcggtatg ttcacctaat
    19021 agcggcgcag ctttaatatc cggcgtaaag gcagagaatt tcattggaca accaacggtc
    19081 agatattttc cacgcaacgg ttgttccact tcaacaacac tgccactttg gcgcaaagag
    19141 ggatcaagtg aaatttcttt catacttaaa accggtgcac aaggaatatc aaactgagtc
    19201 aaataggcca ccgcttcatg tttatcaata gtgacagtgt atttttcgat ttcagcaaaa
    19261 atatcgaaaa tatgtggctg tcgtgcatgg gctgtactgt atgccggatc ggtaatccat
    19321 tctggtttgc cgatggcttt acaggtgttt tcccagtttt gctcctgaat agtgaaataa
    19381 atataggcgt taggatcggt ttcccagcct ttacatttca ggatccagcc aggctgaccg
    19441 ccaccacctg cattaccacc gcggggaact gcatcaccaa atgtaccatt cggatactgc
    19501 gggtattctt ccagataacc caatttatcg agacgctgct ggtcacgtaa tttcacgcgg
    19561 caaaggttca atacggcatc ctgcattgac atggtgactc gttgcccacg ccccgttttt
    19621 tcgcgatgca gcaaagcagc aagtaaaccg atcagcaaat gcattccggt gttgctgtca
    19681 cccaacgctg cagcgcttac cagcggcgga ccatcccaaa aacccgtagt ggatgccgcg
    19741 ccacccgctg cctgagcaac gttttcatag gcttttacat tcacataagg cgaacactca
    19801 tcaaaccctt tgatcgaacc aaaaatcaga cgtggattga tttcttgaat atgctcccag
    19861 gtgaagccca tgtgatcaat ggcccctgga tgaaagttct cgactaagat atcagcttcg
    19921 cggatcagct tttccattac ctctttgcct tccgctgttt tggtatttaa ctcaatagaa
    19981 cgtttgttac tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat atctcgcagc
    20041 tggtgacgcg ttacgtcacc aacgccggga cgttcaattt taataacgtc agcgccaaac
    20101 caggccagca tttgagtaca agatgggcca gattgcacac cggtgaaatc gagaacttta
    20161 attccttgaa gtggagttga catagc
//
LOCUS       DAZFKM010000090        19856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFKM010000090 DAZFKM010000000
VERSION     DAZFKM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..1644)
                     /gene="ybiT"
                     /locus_tag="ACHKAY_003319"
     CDS             complement(52..1644)
                     /gene="ybiT"
                     /locus_tag="ACHKAY_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO5701944.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1863..2783
                     /gene="ldtB"
                     /locus_tag="ACHKAY_003320"
     CDS             1863..2783
                     /gene="ldtB"
                     /locus_tag="ACHKAY_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5701945.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2842..3960)
                     /gene="ybiR"
                     /locus_tag="ACHKAY_003321"
     CDS             complement(2842..3960)
                     /gene="ybiR"
                     /locus_tag="ACHKAY_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO5701946.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3957..4424)
                     /gene="mntR"
                     /locus_tag="ACHKAY_003322"
     CDS             complement(3957..4424)
                     /gene="mntR"
                     /locus_tag="ACHKAY_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO5701947.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4610..4738
                     /gene="mntS"
                     /locus_tag="ACHKAY_003323"
     CDS             4610..4738
                     /gene="mntS"
                     /locus_tag="ACHKAY_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO5701948.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5010..6593
                     /gene="opgE"
                     /locus_tag="ACHKAY_003324"
     CDS             5010..6593
                     /gene="opgE"
                     /locus_tag="ACHKAY_003324"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5701949.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6642..7157)
                     /gene="ompX"
                     /locus_tag="ACHKAY_003325"
     CDS             complement(6642..7157)
                     /gene="ompX"
                     /locus_tag="ACHKAY_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO5701950.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7210..7275)
                     /locus_tag="ACHKAY_003326"
     CDS             complement(7210..7275)
                     /locus_tag="ACHKAY_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701951.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7510..8397
                     /gene="rhtA"
                     /locus_tag="ACHKAY_003327"
     CDS             7510..8397
                     /gene="rhtA"
                     /locus_tag="ACHKAY_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO5701952.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8696..9199
                     /gene="dps"
                     /locus_tag="ACHKAY_003328"
                     /gene_synonym="pexB"
     CDS             8696..9199
                     /gene="dps"
                     /locus_tag="ACHKAY_003328"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO5701953.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9603..10349
                     /gene="glnH"
                     /locus_tag="ACHKAY_003329"
     CDS             9603..10349
                     /gene="glnH"
                     /locus_tag="ACHKAY_003329"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO5701954.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10488..11147
                     /gene="glnP"
                     /locus_tag="ACHKAY_003330"
     CDS             10488..11147
                     /gene="glnP"
                     /locus_tag="ACHKAY_003330"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO5701955.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            11144..11866
                     /gene="glnQ"
                     /locus_tag="ACHKAY_003331"
     CDS             11144..11866
                     /gene="glnQ"
                     /locus_tag="ACHKAY_003331"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO5701956.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11983..14208
                     /gene="ybiO"
                     /locus_tag="ACHKAY_003332"
                     /pseudo
     CDS             11983..14208
                     /gene="ybiO"
                     /locus_tag="ACHKAY_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(14205..15131)
                     /gene="rlmF"
                     /locus_tag="ACHKAY_003333"
     CDS             complement(14205..15131)
                     /gene="rlmF"
                     /locus_tag="ACHKAY_003333"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO5701957.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15407..15667
                     /gene="mcbA"
                     /locus_tag="ACHKAY_003334"
     CDS             15407..15667
                     /gene="mcbA"
                     /locus_tag="ACHKAY_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO5701958.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15931..18213
                     /gene="fiu"
                     /locus_tag="ACHKAY_003335"
     CDS             15931..18213
                     /gene="fiu"
                     /locus_tag="ACHKAY_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO5701959.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18255..18932
                     /gene="ybiX"
                     /locus_tag="ACHKAY_003336"
     CDS             18255..18932
                     /gene="ybiX"
                     /locus_tag="ACHKAY_003336"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO5701960.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19006..19272
                     /gene="ybiI"
                     /locus_tag="ACHKAY_003337"
     CDS             19006..19272
                     /gene="ybiI"
                     /locus_tag="ACHKAY_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO5701961.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYNRRGSKDSQLR"
     gene            19537..19797
                     /gene="ybiJ"
                     /locus_tag="ACHKAY_003338"
     CDS             19537..19797
                     /gene="ybiJ"
                     /locus_tag="ACHKAY_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO5701962.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         4902 a         5202 c         4885 g         4867 t
ORIGIN      
        1 tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca cttactcgat
       61 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
      121 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
      181 cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag
      241 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
      301 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
      361 gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt
      421 ccactgactc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
      481 ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
      541 atcgcccacc agcgttttca gcagcgttga tttaccgacg ccgttggtac ccagtaccgc
      601 aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa
      661 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
      721 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
      781 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
      841 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
      901 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
      961 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
     1021 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
     1081 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag caaacagcgc
     1141 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
     1201 ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt ccgcagagta
     1261 accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt cttcctcact
     1321 catttccggc aaagcataga tgcggtcgcg ctcctgcttc acttcccaca actctttatg
     1381 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
     1441 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct ctaagtcgcc
     1501 gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
     1561 gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat
     1621 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
     1681 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
     1741 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
     1801 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
     1861 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
     1921 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
     1981 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt
     2041 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
     2101 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
     2161 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
     2221 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
     2281 ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgccaaa atgcacgcag
     2341 agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg gataacccga
     2401 tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact
     2461 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
     2521 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
     2581 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
     2641 aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc gtgcaaacag
     2701 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa aaccgctccg
     2761 ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga ctcataatcc
     2821 gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa catatcccac
     2881 caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg
     2941 atcgttcgcc atacgtagcg caatcaaatt tgccagcgat ccgggtaata acccaaagcc
     3001 acctacgttt accgcccata ccagtaataa agacggcggc acatagttca gcaacaatat
     3061 ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc ataacccggg
     3121 ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa
     3181 atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac
     3241 cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc acagctcttg
     3301 tttgaactcc agcgccgtca gaaagacgat atacagcccc agacaactcc acaccagccg
     3361 cggtttccac tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca
     3421 acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg ccatttgggc
     3481 aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc
     3541 aattggcgtc agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt
     3601 aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc
     3661 gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag
     3721 ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct
     3781 taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc
     3841 gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac
     3901 aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat
     3961 ttggcaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg
     4021 tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag
     4081 aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc
     4141 gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta
     4201 agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga
     4261 gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg
     4321 cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac
     4381 tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat
     4441 ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt
     4501 acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta
     4561 atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt
     4621 caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc
     4681 tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg ataaatagcg
     4741 gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt ttggtctgaa
     4801 aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt
     4861 gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat
     4921 gcggacccct ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa
     4981 gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta
     5041 cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta
     5101 acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc
     5161 ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtatc agttcgctgg
     5221 tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaacttc aatacattgc
     5281 tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt
     5341 acagctacat ggtcggcata tttatttttg tgctcggcgt aatagcaatc aggcgaaaaa
     5401 aagagagtga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg
     5461 cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag
     5521 atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga
     5581 ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg
     5641 ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg cgtcgcgatg
     5701 ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt
     5761 tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc
     5821 tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc
     5881 gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg
     5941 ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg
     6001 aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc
     6061 aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct
     6121 gcgacaggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc
     6181 tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca
     6241 gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg
     6301 gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt
     6361 ttatggtcat ttccagcgac gataaagcgc atcgggtgat taaagcccgc cgctcagcca
     6421 atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca
     6481 aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac
     6541 agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaacaaaa
     6601 aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg gtaaccaaca
     6661 ccggcaatcc aggtacctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc
     6721 agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg
     6781 ctggtgtcgt gtttgtaggt cgggtattcc gtggtctgga atttaccata acccaccccc
     6841 actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg
     6901 tagtactggt ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg
     6961 aaagagccga tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa
     7021 ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta
     7081 gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga
     7141 catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata
     7201 tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta
     7261 acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc
     7321 gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa
     7381 tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc
     7441 tcaaccatca ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg
     7501 ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat aatcatattg
     7561 ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg
     7621 gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg
     7681 ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac
     7741 ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg
     7801 ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt
     7861 cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg
     7921 ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg
     7981 gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg
     8041 acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag
     8101 gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt cgctattctc
     8161 tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca
     8221 tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc
     8281 ggagaaacac tgacacccat acagctgctg gcgctcggcg ctatcatcgc cgcttcaatg
     8341 gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt
     8401 tacatttctg catggttatg cataaccatg cagaatttct cgctactttt cctctacacc
     8461 gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagcagat
     8521 aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag ttttgttttt
     8581 cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg
     8641 tgctatactt agtctcgtta attactggga cataacatca agaggatatg aaattatgag
     8701 taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc
     8761 cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga
     8821 tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt
     8881 acatgaaatg ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga
     8941 acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac
     9001 cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga aagaactggc
     9061 tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga
     9121 cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat
     9181 cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac ccgataaaag
     9241 cggcttcctg acaggaggcc gttttgtttt gcagcccacc tcaacgcact tatttagtgc
     9301 atccatctgc tatctccagc tgattaagta aattttttgt atccacatca tcacacaatc
     9361 gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca
     9421 caattttagc gcaccagatt ggtgccccag aatggtgcat cttcagggta ttgccctata
     9481 aatcgtgcat cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa actggcacga
     9541 ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg
     9601 ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggctttt gcggtttctt
     9661 ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat
     9721 ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc atcgctaaag
     9781 agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc
     9841 aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag cgtaaaaaag
     9901 cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca
     9961 ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggta
    10021 ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga
    10081 acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt ctgcacgata
    10141 cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg
    10201 actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg
    10261 acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca
    10321 aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaaacg cactggcagt
    10381 tccctctccc ctctggggag aggattaggg tgaggggcgc aaacccgctc cggggccatt
    10441 aattaccctg aatttgatta tttacaccac ggtaacagga acaacatatg cagtttgact
    10501 ggagtgccat ctggcctgcc attccgcttc tgattgaagg tgccaaaatg accctgtgga
    10561 tttcggtcct cggtctggca ggcggtctgg taatcggatt gctggcaggt tttgcacgca
    10621 ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt tattgaagtg atccgcggca
    10681 cacctatcgt cgtccaggtg atgtttatct atttcgccct gccgatggcg tttaacgact
    10741 tacgcatcga cccatttact gcggcggtgg tcaccatcat gatcaactcc ggcgcgtaca
    10801 ttgcggaaat cacgcgtggt gcggtgctgt ctatccacaa aggttttcgt gaagcaggac
    10861 tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt cattttaccg ctggcactgc
    10921 gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag cattaaagac acctcgctgt
    10981 ttattgtgat cggcgtggcg gaactgaccc gtcaggggca agaaattatt gccggtaact
    11041 tccgcgccct tgagatctgg agcgccgtgg cggtgctcta tctgattatt accctggtgc
    11101 tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg aatttaaaaa
    11161 cgtctccaag cactttggcc caacccaggt gctgcataat atcgatttga acattgccca
    11221 gggcgaagtc gtggtgatta tcgggccgtc cggttccggt aaatcgaccc tgctgcgctg
    11281 catcaacaaa ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc tgaaggttaa
    11341 cgatccgaaa gttgacgagc gcctgattcg ccaggaagca ggtatggtgt tccagcagtt
    11401 ttacctcttc ccgcatctga cggcgctgga aaacgtcatg tttggtccgc tacgcgtgcg
    11461 tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga aagtcggtct
    11521 ggcagaacgt gcacatcact acccttccga actttctggt ggtcaacagc agcgtgtggc
    11581 gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac cgacttccgc
    11641 tcttgacccg gaactgcgcc atgaagtgct gaaggttatg caggatctgg ctgaagaagg
    11701 gatgacgatg gtgatcgtga cccacgaaat cggttttgcc gagaaagtag cttcgcggct
    11761 gatctttatc gacaaaggcc ggattgcgga agatggcgat ccgcaggtat tgatcaagaa
    11821 cccgccgagc cagcgcttgc aggaattttt gcagcacgtc tcttaacttc tctgcctccc
    11881 tttcacccga aagggaggca caccagattc ctctcattta aaatcgcccc tcccccagca
    11941 tctatactta tctttttgct ctgttttctc actggaggag tcatgcggtg gatcctgttc
    12001 atcctcttct gcctgctggg cgcacctgcc cacgcggtat ccatacccgg cgttacaacc
    12061 acaacgacaa cggactcaac gactgaaccg gccccggaac cggatatcga acaaaaaaaa
    12121 gcggcctatg gcgcactggc ggatgtgctg gataacgaca cctcgcgtaa agaattgatc
    12181 gaccagttgc gtactgtcgc cgcgacgccc cctgcggaac cggtcccgaa gatcgtgccg
    12241 ccgacgctgg ttgaagagca aaccgtgctg caaaaggtca ccgaagtcag ccgccattat
    12301 ggtgaagccc tttccgcccg cttcgggcaa ctttatcgca atatcaccgg ctccccgcat
    12361 aagccattta atccacaaac cttcagcaat gcgctgagcc atttttcaat gttagcggta
    12421 ttagtgtttg gtttttacta gctgattcgc ctgtgcgcac tgccgctgta tcgcaaaatg
    12481 ggtcagtggg cgaggcaaaa aaatcgtgag cgcagcaact ggttgcagct tccggcgatg
    12541 attatcgggg cgtttattat cgacctgctg ttactggccc tgacattatt tgtcggccag
    12601 gtattaagcg acaacctgaa tgcaggcagt cgcaccatcg ctttccaaca aagtttgttt
    12661 ctcaatgcct ttgccctgat tgaatttttc aaagccgtac tacgcctgat tttttgccca
    12721 aacgtggcgg agctgcgtcc gttcacgatt caggacgaga gcgcccgtta ctggagtcgt
    12781 cgcctgagct ggttaagcag tttgataggt tatggcctga ttgtggccgt gccgattatc
    12841 tctaatcagg tgaatgtaca gataggtgcg ctggcgaacg tcatcattat gctgtgcatg
    12901 accgtctggg cgttgtacct gatctttcgt aataaaaaag agattaccca gcatttgctc
    12961 aactttgcgg agcattcgct ggcctttttc agcctgttta tccgcgcctt tgcgctggtg
    13021 tggcactggc tggcaagcgc ctattttatc gtgctgtttt tcttttcgtt gttcgatccg
    13081 ggcaacagcc tgaaatttat gatgggtgca acggtgcgca gcctggcgat tattggtatc
    13141 gcagcgtttg tttccggtat gttttcccgc tggctggcga aaaccatcac cctctcgcca
    13201 catactcagc gtaactatcc ggagctacaa aaacggctga atggctggct ttcggcggcg
    13261 ctgaaaacgg cgcgtattct gaccgtctgc gtggcagtaa tgttgctgtt gagcgcatgg
    13321 ggattgttcg atttctggaa ctggctgcaa aacggcgcgg ggcagaaaac cgtagatatc
    13381 ctgatccgca tcgcactcat tcttttcttc tcggcggttg gctggacggt gctcgccagt
    13441 ttgatcgaaa accggctggc ttcggatatt catggccgcc cgctacccag cgcccgtacg
    13501 cgtaccctgc tgacgctgtt tcgtaacgcg ctggcggtga ttatcagtac catcaccatc
    13561 atgattgtgt tgtcggaaat cggcgtcaat atcgcgccat tgctggcagg tgccggggca
    13621 ttaggtctgg ctatctcgtt tggttcgcaa acgctggtga aagatatcat taccggggtg
    13681 tttattcagt ttgaaaacgg catgaacact ggagatttgg tgactatcgg gccgttgacc
    13741 ggcacagtgg aacggatgtc gattcgctcc gtgggcgtgc gccaggatac cggggcgtat
    13801 cacatcattc cgtggtcttc gataaccacc tttgctaact tcgtccgcgg tattggttcg
    13861 gtagtggcaa actacgatgt tgatcgccat gaagatgctg ataaagccaa tcaggcactg
    13921 aaagatgcgg tagcggaatt gatggaaaac gaagaaattc gcgggctgat tattggtgaa
    13981 ccgaattttg ccgggattgt cggcttaagc aataccgcgt ttacactgcg tgtttcgttc
    14041 accacgctgc cactcaaaca gtggacggta cgctttgccc tcgacagcca ggtgaaaaaa
    14101 catttcgacc tggcgggcgt tcgcgcgcca gtgcagactt atcaggtgct gcctgctccg
    14161 ggcgcgaccc cggctgaacc gccgccgccg ggggagccaa cgctttaacg ttggcgatta
    14221 acaaaacggc ggcgctgctc gtcgttcata aaggtccagg caataaagcg actctgcttt
    14281 tgcccctggg ccatctcttt ttttaccacc ttcaccgcgc ccacgtcggt cagagcacga
    14341 tacaacggcg gtaagttttc accacgagat accagcgagg taaaccacat cacctgcttc
    14401 gcgaagcctt tgctctcttc aatcatcttt ttgataaagg cgacttcacc accttcacac
    14461 cacaactcct gttgctgacc accaaagttc agtgcatcgt ctttgttcag ccccaggtta
    14521 cggcgtttac gctcactacc tgcccgcgct gcagcggcgg aatcgtggaa tggcgggtta
    14581 cacaaggtcg cgtcgtattg ctcgttttta tggatgatgc cgttaaaaat tgccccactc
    14641 tctttttgcc gacgcagacg aatggcgcgg ttaagccccg gattagcact gataatcgcc
    14701 tgcgcactgc ttaacgcctg gctgctggtt tcactaccgg taaaacgcca gccatattca
    14761 tgtacgccaa tcagcggata aatacagttc gcaccaacgc cgatatccag aatgctggca
    14821 ttcgctggaa ttgttccgct tgcttcagcc agtaaatcgg caaggtgatg aatataatcc
    14881 gcccggcccg gtactggtgg gcagagaaaa ccgtcgggga tatcccagtt cgctacggcg
    14941 taaaaatggg ccagcaacgc cttattgagc gccttcaccg ccagcggatt ggcaaagtct
    15001 acgctttgct ccccggcggg tgtaagcgtg aggaattgcc tgagttcagg attgacctga
    15061 caaagcgtgg cgagatcgta gcggctgtga tgacggttgc gcggatgcaa ccccggtttc
    15121 tgggcggaca tagcattctc ctttaagcag cggcgtaaga tacccgcata acgcgacagg
    15181 gtaaatatct ggctgcgttt atcattcccc aaaaccttaa catggctatg gtttatggca
    15241 attcatcata aaaatcagca ttatgcatgt cattgatttg tcttattttg taaaaatata
    15301 tcgttcaaaa aataagcaca tctctgtcaa ttttttgcgt cattttcatc gcccaggcga
    15361 cctacactta tcaggcacta cccgttgtat ttatgaggat gtcattatga aaaagtgcct
    15421 cacactactg attgccaccg tcctgagcgg aatctctctc acggcttatg ccgcgcaacc
    15481 gatgagtaac ctggacagcg gtcagctacg gcccgccggt accgtttcgg caactggcgc
    15541 atcaaaccta agcgatctgg aggataaact ggcagaaaaa gcgcgcgagc aaggcgcgaa
    15601 aggttatgtc attaattccg caggcggaaa tgaccagatg ttcggtactg caaccatcta
    15661 caaataaccc caccaacgta actgcattcg cctctggatg cagttttcgc ctgttttgta
    15721 atcaaaaaac atcaaatatg aacgcaatgt aaataaatgt atttcttttt cgcgcaatgg
    15781 gtgatagaaa atcgcgccaa gtgataatgc ttatcaaaat tattatcact tgcacgaaca
    15841 ctatcacgag attaacagtg gcatcgcatc cgcagagagg ctttctcgtg gcagtgaaaa
    15901 tttcaacata taaaaaaagt cacctgcaaa atggaaaaca atcgcaattt ccctgccaga
    15961 caatttcatt cgctcacgtt ctttgccggt ctttgtattg gcatcacgcc tgtggctcag
    16021 gcactcgccg ccgaagggca aactaacgcg gatgacacgc tggttgtcga agcatcaacg
    16081 ccttcgcttt atgcgccaca acaatctgcc gatccgaaat tctcgcgtcc ggtagcggat
    16141 actacccgca cgatgacggt gatttctgaa caagtgatta aagatcaggg cgcaaccaac
    16201 cttaccgacg cgctcaaaaa cgtccccggc gtgggtgcgt tttttgcggg tgagaacggt
    16261 aactccacca ctggcgacgc catttatatg cgcggtgctg atacctctaa cagtatttat
    16321 attgatggca ttcgcgatat cggcagcgtc tcgcgcgaca ccttcaatac tgagcaggtc
    16381 gaagtgatta aagggccttc cggcaccgac tacgggcgca gcgcgccgac gggctcgatc
    16441 aatatgatca gcaagcagcc gcgcaatgat tccggcattg acgcctccgc cagtattggc
    16501 agcgcctggt tccgccgcgg cacgctggac gtcaatcagg tcattggtga taccactgcg
    16561 gtgcgcctga atgtgatggg cgaaaaaacg cacgatgccg gacgcgacaa agtcaaaaat
    16621 gagcgttacg gcgtcgcccc ttctatcgct tttggccttg gtacagcgaa tcgtttgtat
    16681 cttaattatc tgcatgtcac ccagcacaac acgccagacg gcggcattcc gaccatcggt
    16741 ttgccgggtt attctgcccc atctgcggga acagcggctc tgaatcattc cggaaaagtt
    16801 gatactcata acttttacgg cacggattcc gattacgacg attcgaccac cgacaccgcc
    16861 accatgcgtt ttgagcacga catcaacgat aacaccacca ttcgcaatac tacccgttgg
    16921 tcgcgcgtaa agcaggatta cctgatgacg gcgattatgg gcggggcgtc gaatattact
    16981 cagcccacca gcgatgtgaa tagctggacc tggtcacgca cggcgaatac caaagatgtg
    17041 agtaataaaa ttctcaccaa ccagaccaac ctgacctcga cgttctatac cggttctatc
    17101 ggtcatgatg tcagtaccgg cgtggaattt acccgtgaaa cgcagactaa ctacggcgtt
    17161 aatccggtga cgttacccgc ggtaaatatt tatcatcctg acagcagcat tcatcccggc
    17221 ggcctgacgc gcaacggcgc aaacgccaat ggtcagacgg ataccttcgc aatttacgcc
    17281 ttcgatacgc tgcaaatcac ccgtgatttt gagctgaacg gcgggatccg tctggataat
    17341 tatcatactg aatatgacag tgccaccgcc tgcggcggca gcggacgcgg tgccatcacc
    17401 tgcccaactg gtgtggcaaa aggttctccg gtcaccaccg tcgacaccgc caagtcgggc
    17461 aatctggtga actggaaagc cggggcgctg tatcacctga cggaaaacgg taatgtctat
    17521 attaactatg ccgtttccca gcagcctccg ggcggcaaca acttcgccct tgcgcagtct
    17581 ggcagcggta acagtgccaa ccgcaccgat tttaaaccac aaaaagccaa caccagcgag
    17641 attggcacca aatggcaggt tctggataaa cgcctgttgc tcaccgccgc gctattccgt
    17701 actgatatcg aaaatgaagt tgagcaaaat gatgacggaa cttactcgca atacggtaag
    17761 aaacgtgtcg aaggctatga gatatccgtg gccgggaata tcacgcccgc gtggcaggtg
    17821 attggcggct atacccagca aaaagcaacc atcaaaaacg gcaaagatgt tgcccaggat
    17881 ggttcctcat cgctgccgta taccccggag cacgccttca ccttatggag ccagtatcag
    17941 gcaacagatg atatctctgt tggcgcgggc gcacgctata tcggcagtat gcataaaggt
    18001 tcagacggcg cggtgggaac gccagcgttt accgaaggtt actgggtcgc cgatgccaaa
    18061 ctggggtatc gggttaatcg caatctcgac ttccagctaa acgtttacaa cctgtttgat
    18121 accgattacg tcgcctcaat caacaagagc ggctaccgtt atcacccggg cgagccaaga
    18181 accttcttgc tcacagccaa tatgcatttc tgattcagat gtggggcgca ggccccactt
    18241 tttggagaac ttgtatgatg taccacattc ccggcgtgtt atcgccacag gacgtcgccc
    18301 ggtttcgcga acaactggaa caagccgaat gggtggatgg acgcgtcacc accggcgcac
    18361 aaggtgcgca agttaagaac aatcaacagg tcgacacccg cagcacgtta tacgccgccc
    18421 tgcaaaatga ggtgctgaac gcggttaacc aacatgcttt attctttgcc gcggccttgc
    18481 cgcgtaccct ttccacgccg ctgtttaatc gctatcagaa caatgaaacc tatggtttcc
    18541 atgtggatgg cgcagtacgc agccatccac aaaacggctg gatgcgtact gacctttctg
    18601 ccacgctgtt tttaagcgat ccacaaagct acgacggcgg cgaactggtc gttaatgaca
    18661 ccttcggaca acatcgggta aaactcccgg caggcgatct cgtgttgtat ccctccagca
    18721 gcctgcattg cgtgacaccc gtaacccgcg gcgtacgagt ggcatcattt atgtggatcc
    18781 agtcgatgat ccgtgatgat aaaaagcgcg ccatgctgtt tgaactggac aacaatattc
    18841 agtcgctgaa aagccgctac ggcgaaagtg aagagatcct gtcgctgctt aatctttatc
    18901 ataatctgct gcgggaatgg tcggagatct gatgcttgaa ctgcccaggt tcgtccctgg
    18961 attagctaca cttaactgta caagcattga tatggggagg acgatatggc atccggttgg
    19021 gctaacgatg acgccgtcaa cgaacagatc aacagtacaa ttgaagatgc gattgcccgc
    19081 gctcggggtg aaattccgcg tggcgaaagc ctggatgaat gtgaagagtg cggtgccccc
    19141 atcccgcaag cccgtcggga agccattcct ggcgtgcgct tatgcattca ttgtcagcag
    19201 gagaaaggtt tacaaaaacc agcgtataca ggatataatc gcagaggttc gaaagacagc
    19261 cagttacgtt aactacaact ggcgagatgc atagcgagta taatttgtat tttgcgtggc
    19321 attccgtgcc tttaacgctg ctgtgactgc cacgcggcat aaaacaagtc cataagtaca
    19381 acaaataaat ggtttatcag tatgttagat atcaatcaat ttatttgaac aaggcggtca
    19441 attctcttcg attttatctc tcgtaaaaaa acgtgatact catcacatcg acgaaacaac
    19501 gtcacttata caaaaatcac ctgcgagaga ttaattatga aaactatcaa tactgttgtt
    19561 gctgctatgg ctctttcaac tctgtcattt ggcgtgtttg ccgcggaacc ggtaacggca
    19621 tcccaggcac agaacatgaa taaaatcggc gtggtttctg ccgatggcgc atccaccctc
    19681 gatgccctgg aagcgaaact ggctgagaaa gccgcggcag ccggtgccag tggatacagc
    19741 atcacttccg ccaccaacaa caataaatta agcggtactg cggtaattta taagtaattc
    19801 gtaggacgga taaggcgttt acgccgcatc cggcaacaga tgcctgatgc gacgct
//
LOCUS       DAZFKM010000091        19714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFKM010000091 DAZFKM010000000
VERSION     DAZFKM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2461
                     /locus_tag="ACHKAY_003339"
     CDS             <1..2461
                     /locus_tag="ACHKAY_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFO5701963.1"
                     /translation="KLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTISGPAF
                     NEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVLVAF
                     VLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYLLK
                     EETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHLL
                     NTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQ
                     EAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKM
                     ISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLE
                     PLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADG
                     MRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLL
                     EQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLN
                     DIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGR
                     INKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLG
                     TLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLA
                     ELVTKGHLRPLKEASEAENVA"
     gene            2500..2682
                     /locus_tag="ACHKAY_003340"
                     /pseudo
     CDS             2500..2682
                     /locus_tag="ACHKAY_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(2795..3631)
                     /locus_tag="ACHKAY_003341"
     CDS             complement(2795..3631)
                     /locus_tag="ACHKAY_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO5701964.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3631..4434)
                     /locus_tag="ACHKAY_003342"
                     /note="aph(3'')-Ib"
     CDS             complement(3631..4434)
                     /locus_tag="ACHKAY_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO5701965.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(4495..5310)
                     /gene="sul2"
                     /locus_tag="ACHKAY_003343"
     CDS             complement(4495..5310)
                     /gene="sul2"
                     /locus_tag="ACHKAY_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO5701966.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5647..5850
                     /locus_tag="ACHKAY_003344"
     CDS             5647..5850
                     /locus_tag="ACHKAY_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701967.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGID
                     TLDGTTRTALDSVISKLKDEVWP"
     gene            5841..6062
                     /locus_tag="ACHKAY_003345"
     CDS             5841..6062
                     /locus_tag="ACHKAY_003345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701968.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDG
                     CTLERAEVAFLDEDGPSPDLPHRVTLPRD"
     gene            complement(6297..7283)
                     /gene="mobV"
                     /locus_tag="ACHKAY_003346"
     CDS             complement(6297..7283)
                     /gene="mobV"
                     /locus_tag="ACHKAY_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFO5701969.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHL
                     AARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWL
                     ADKYGAENVVTATIHRDELTPHLSAFVVPRTADGRLSAKEFIGNRTKMSQDQTSYAER
                     VADLGLERGIEGSRATHQRVKTHYGAIQQAGRDVPHLTPDELNPQKVKGVSLAEKVFG
                     AVETVEGVAQRLNAKIMGSVQPMAEKAAVSAQNERRAKELRETLAQQQKRLQALQEPF
                     KGLSKDQVAGLIRQAVKLRQENEQEKQERAQQIKERFKAKRERERSDRSRGR"
     gene            complement(8753..9088)
                     /locus_tag="ACHKAY_003347"
     CDS             complement(8753..9088)
                     /locus_tag="ACHKAY_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701970.1"
                     /translation="MRTITTREQLLVNGKVRERIATHIVTGAHGYETLCTSGYNLQYN
                     KERVLIENCEKVADGELPVTCHTCFSIWQDVHRFKPGDFDTESGKGNFTDTELTKITI
                     GQEKTPNAC"
     gene            9426..10406
                     /locus_tag="ACHKAY_003348"
     CDS             9426..10406
                     /locus_tag="ACHKAY_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5701971.1"
                     /translation="MRIFVDDGSTNIKLAWVEDGAVKTLISPNSFKPEWSMTLQGLTD
                     ATVPASANYEIDGEKYSFDQLSPDAVRTTETRYQYSDVNVVAIHHALMQSGIAPQPVD
                     IVVTLPLGEYLDENDQPNLANIERKKNNVRRTVTVQGRENFTIRKVSVLPESVPAGFD
                     VLKDLNDLESLLIVDIGGTTLDIAHVRSKMSGFTKTYCDPKTGVSIITEAVKHAMDVN
                     VSTRTSSYFADRLIQGRDDNEFLTRYIPNAEQRERILTVLRDREKKLQHRVADSVGRF
                     AGFTHVMVVGGGASLVAGAVKKATGVGDNRFFVSDNPQFDLVLGMMAMKG"
     gene            10409..10831
                     /locus_tag="ACHKAY_003349"
     CDS             10409..10831
                     /locus_tag="ACHKAY_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5701972.1"
                     /translation="MADNRKCSFYIYPERNAADRVADRFLEKLPQKERGRAMRAMMLC
                     GAALMKQDERLPFLIAEFLTDSTSMQDIQRIISSTLPQQENGEVVRLLEAFLQSAGNN
                     AKTILPAVDSATQEISAPVDQNLLETRNNIKNLFPDDE"
     gene            10855..11145
                     /locus_tag="ACHKAY_003350"
     CDS             10855..11145
                     /locus_tag="ACHKAY_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701973.1"
                     /translation="MLRPFFFSDKECRWAPLFTAPADAARQERCLWSVSRVRRARDGR
                     FYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            11255..12157
                     /locus_tag="ACHKAY_003351"
     CDS             11255..12157
                     /locus_tag="ACHKAY_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5701974.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSLENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVTHYGG"
     gene            12154..12465
                     /locus_tag="ACHKAY_003352"
     CDS             12154..12465
                     /locus_tag="ACHKAY_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701975.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            12542..13225
                     /locus_tag="ACHKAY_003353"
     CDS             12542..13225
                     /locus_tag="ACHKAY_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5701976.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFGVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            13226..13447
                     /locus_tag="ACHKAY_003354"
     CDS             13226..13447
                     /locus_tag="ACHKAY_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701977.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            13377..13895
                     /locus_tag="ACHKAY_003355"
     CDS             13377..13895
                     /locus_tag="ACHKAY_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5701978.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQY
                     TENTPLMLVVWSPEEIQALADGMEISLTGHEIRTVLARLEDIPEEQRIESGISSAAVM
                     EIINNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQEVVNQA
                     RTLLKNNTHEND"
     gene            13940..14710
                     /locus_tag="ACHKAY_003356"
     CDS             13940..14710
                     /locus_tag="ACHKAY_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701979.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWVSIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEYDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVLEARSEIRSMADALAKIRG"
     gene            15123..15548
                     /locus_tag="ACHKAY_003357"
     CDS             15123..15548
                     /locus_tag="ACHKAY_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5701980.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            15595..16017
                     /locus_tag="ACHKAY_003358"
     CDS             15595..16017
                     /locus_tag="ACHKAY_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO5701981.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            16014..16205
                     /locus_tag="ACHKAY_003359"
     CDS             16014..16205
                     /locus_tag="ACHKAY_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701982.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIIDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(16484..16732)
                     /locus_tag="ACHKAY_003360"
     CDS             complement(16484..16732)
                     /locus_tag="ACHKAY_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701983.1"
                     /translation="MSRGPLQRRRARARNGVAPYIPEAFCRDAGSAGHGATEAARPER
                     SLQAGGFHASDRSDSPKPRQRVRRRLAGAPAAACRSRA"
     gene            17247..17477
                     /locus_tag="ACHKAY_003361"
     CDS             17247..17477
                     /locus_tag="ACHKAY_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701984.1"
                     /translation="MTISIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            17529..18890
                     /locus_tag="ACHKAY_003362"
     CDS             17529..18890
                     /locus_tag="ACHKAY_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFO5701985.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     DGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            18937..19500
                     /locus_tag="ACHKAY_003363"
     CDS             18937..19500
                     /locus_tag="ACHKAY_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO5701986.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFIHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDIREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(19586..>19714)
                     /locus_tag="ACHKAY_003364"
     CDS             complement(19586..>19714)
                     /locus_tag="ACHKAY_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5701987.1"
                     /translation="SSSSSSSSSSSRNNNIISNRQMTGMKRQKHTAEHPRNERGGA"
BASE COUNT         4380 a         5266 c         5623 g         4445 t
ORIGIN      
        1 caaactggca ctgataccgt cagccgaaca gcgttcacag ctggagatgc tgctggggcc
       61 aactgattgc agccgcctgt ctttactgga atcactgaaa aagggccctg tgaccatcag
      121 tggtccggcg tttaatgaag caattgaacg ctggaaaact ctgaacgatt ttggcctgca
      181 tgctgaaaac ctgagtacac tcccggctgt gcgcctgaaa aatctcgcac gttatgctgg
      241 tatgacttcg gtgttcaata ttgccaggat gtcaccgcag aaaaggatgg cggttctggt
      301 tgcctttgtc cttgcatggg aaacgctggc gctggatgat gcattggacg ttctggacgc
      361 catgctggcc gttatcatcc gtgacgccag aaagattggg cagaaaaaac ggctccgctc
      421 gctgaaggat ctggataaat ctgcattggc gctcgccagc gcatgttcgt acctgctgaa
      481 agaagaaaca ccggacgaat cgattcgtgc tgaggtgttc agctacatcc caaggcaaaa
      541 gctggctgaa atcatcacgc ttgtccgtga aattgcccgg ccctcagacg ataattttca
      601 tgaagaaatg gtggagcagt acgggcgcgt tcgtcgtttc ctgccccatc tgctgaatac
      661 cgttaaattt tcatccgcac ctgccggggt taccactctg aatgcctgtg actacctcag
      721 ccgggagttc agctcacggc ggcagttttt tgacgacgca ccaacggaaa ttatcagtcg
      781 gtcatggaaa cggctggtga ttaacaagga aaaacatatc acccgcaggg gatacacgct
      841 ctgctttctc agtaaactgc aggatagtct gaggcggagg gatgtctacg ttaccggcag
      901 taaccggtgg ggagatcctc gtgcaagatt actacagggt gctgactggc aggcaaaccg
      961 gattaaggtt tatcgttctt tggggcaccc gacagacccg caggaagcaa taaaatctct
     1021 gggtcatcag cttgatagtc gttacagaca ggttgctgca cgtctttgcg aaaatgaggc
     1081 tgtcgaactc gatgtttctg gcccgaagcc ccggttgaca atttctcccc tcgccagtct
     1141 tgatgagccg gacagtctga aacgactgag caaaatgatc agtgatctac tccctccggt
     1201 ggatttaacg gagttgctgc tcgaaattaa cgcccatacc ggatttgctg atgagttttt
     1261 ccatgctagt gaagccagtg ccagagttga tgatctgccc gtcagcatca gcgccgtgct
     1321 gatggctgaa gcctgcaata tcggtctgga accactgatc agatcaaatg ttcctgcact
     1381 gacccgacac cggctgaact ggacaaaagc gaactatctg cgggctgaaa ctatcaccag
     1441 cgctaatgcc agactggttg attttcaggc aacgctgcca ctggcacaga tatggggtgg
     1501 aggagaagtg gcatctgcag atggaatgcg ctttgttacg ccagtcagaa caatcaatgc
     1561 cggaccgaac cgcaaatact ttggtaataa cagagggatc acctggtaca actttgtgtc
     1621 cgatcagtat tccggctttc atggcatcgt tataccgggg acgctgaggg actctatctt
     1681 tgtgctggaa ggtcttctgg aacaggagac cgggctgaat ccaaccgaaa ttatgaccga
     1741 tacagcaggt gccagcgaac ttgtctttgg ccttttctgg ctgctgggat accagttttc
     1801 tccacgcctg gctgatgccg gtgcttcggt tttctggcga atggaccatg atgccgacta
     1861 tggcgtgctg aatgatattg ccagagggca atcagatccc cgaaaaatag tccttcagtg
     1921 ggacgaaatg atccggaccg ctggctccct gaagctgggc aaagtacagg tttcagtgct
     1981 ggtccgttca ttgctgaaaa gtgaacgtcc ttccggactg actcaggcaa tcattgaagt
     2041 ggggcgcatc aacaaaacgc tgtatctgct taattatatt gatgatgaag attaccgccg
     2101 gcgcattctg acccagctta atcggggaga aagtcgccat gccgttgcca gagccatctg
     2161 tcacggtcaa aaaggtgaga taagaaaacg atataccgac ggtcaggaag atcaactggg
     2221 cacactgggg ctggtcacta acgccgtcgt gttatggaac actatttata tgcaggcagc
     2281 cctggatcat ctccgggcgc agggtgaaac actgaatgat gaagatatcg cacgcctctc
     2341 cccgctttgc cacggacata tcaatatgct cggccattat tccttcacgc tggcagaact
     2401 ggtgaccaaa ggacatctga gaccattaaa agaggcgtca gaggcagaaa acgttgctta
     2461 acgtgagttt tcgttccact gagcgtcaga ccccttctac gggttcgctg cgagtcttgc
     2521 cacgccgagc ccctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg
     2581 gcgcggcgga tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc
     2641 atgcgtcagt gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc
     2701 gggaaggggc ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat
     2761 gtgttcccag gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct
     2821 tgatcgcggc cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg
     2881 cggaaaggca cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg
     2941 cacgagagaa tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc
     3001 tgtcagccgg atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga
     3061 cgggatctat caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc
     3121 catgtagccc acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt
     3181 ggacgtagtc agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg
     3241 caaagcgatc ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca
     3301 gcttcgccat tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga
     3361 tgtgagagag cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga
     3421 gcaaccggac tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat
     3481 cagcaatgtc ttttataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg
     3541 aaactttcca aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc
     3601 aatgagcagg aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag
     3661 atagaaggca aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc
     3721 gaaggcgcgc tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag
     3781 tgccaaatcg gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca
     3841 ttgaagagtt ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac
     3901 catatcggtg cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag
     3961 atcgagctgc ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg
     4021 ggacaccaca tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg
     4081 atcaaccgat aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag
     4141 caaatccgct ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca
     4201 tgcaccctcc tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt
     4261 gagccaaatg aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat
     4321 tttcgcgaag gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct
     4381 gacaggcaac cagtcagaat gcgattcacc aaaaaaaata ttagttcgat tcaatggagg
     4441 ttccttcagt tttctgatga agcgcgaata tagagaaata tcccgaatgt gcagttaacg
     4501 aattcttgcg gtttctttca gcgccgccaa taccgccagc ccgtcgcgca aggggcgcgg
     4561 ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg gcaagctctg cagcgagtgt
     4621 cgcggccccg acatcccccg gaccacggcc tgtgagcgcg cgcagaaagg atttgcgcga
     4681 aacagacaga agcaccggca aatcgaagcg cagccgcaat tcatcgaacc gcgccagcac
     4741 cgagagcgag gtttcgggag cagcccccag aaaaaacccc atgccgggat caaggacaag
     4801 gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg cgcgcgtcaa agaacgccgc
     4861 aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga tctgcctgcc cgtcttgcac
     4921 cgaatgcata acgacgagtt tggcagatga tttcgccaat tgcggataga acgcagcgtc
     4981 tggaaaaccg cgaatatcat tgagataggc cacaccacgc gacaaggcat aggcttgcgt
     5041 cgcgggttga taactgtcga gcgagacggg aatgccatct gccttgagcg cgtccagcac
     5101 cggcgcgata cgcgcgattt ctgtgtcgga cgaaacaggc gcggcgtcgg gattgctgga
     5161 tgccggaccg aggtcgatca catctgcccc ctcggccatc agcttacgcg cctgcgcaat
     5221 ggctgcgtct ggcgccagat accggcctcc atcggagaaa ctgtccgagg ttatgttgac
     5281 gatgccgaaa atgatgagcg atttattcat gggggcttct ataataataa taatcgagca
     5341 tgagtatcat acggatgctc gggtcgaaag ggaatcccca ggcgagtaac ctgtttgcgg
     5401 tgatccatta gctgcaggag cagaagagca tacatctgga agcaaagcca ggaaagcggc
     5461 ctatggagct gtgcggcagc gctcagtagg caatttttca aaatattgtt aagccttttc
     5521 tgagcatggt atttttcatg gtattaccaa ttagcaggaa aataagccat tgaatataaa
     5581 agataaaaat gtcttgttta caatagagtg ggatacagac acgcttgata tgtttggaga
     5641 tcagccatga cagatccact caaagccctt ttcggcaagc ctgattactc ccatatcgta
     5701 agggacacca cggccactat cagcatcaca gccgctgaaa tggccgctgt tttggaggct
     5761 tacgacaggg gtatagacac gctggacggc accaccagaa cggcgctgga ttccgttata
     5821 tcgaaactca aggacgaggt atggccatga gcatgtatta catcaagaca ggtctggagt
     5881 actggaaacc caagggctac gggtacacca agagccgcga ggaggcgggg gttttcaccc
     5941 tcaaggacat ggaagaattg aacctcgatg gctgcaccct ggaacgcgcc gaagtggcgt
     6001 tcctggacga ggacggcccc agtcccgacc taccccaccg cgtcaccctg ccccgcgatt
     6061 gagcggggcg ctggagggtt tcaaaggggc accctttggc acgggatatg tgtcaaaaac
     6121 ggagtttttg taaacatatc cgacgtgcct gtaaatccgc gcgattactg ccagccctta
     6181 caccccggtt gcttagggtt ggttgaaagt ggtttagggt tggttgaaag tggtttaatg
     6241 ttggttaaac aatggtttaa ggttgttcaa ggttggttaa agttggttga aaagggttaa
     6301 cggcccctgc tgcggtcgct gcgctcccgt tcccgcttgg ccttgaatcg ctctttgatc
     6361 tgctgggcgc gctcctgctt ctcctgctcg ttctcctgcc gcaatttcac cgcctgccgg
     6421 atcagtcccg ctacctgatc cttggatagc cccttaaacg gctcctgaag ggcctgtaag
     6481 cgtttttgct gctgggctag ggtttccctt agctccttgg ctcttcgctc gttctgggcg
     6541 cttacagcgg ctttctcggc catcggctgc acagacccca ttattttggc gttcaacctt
     6601 tgcgccaccc cttcgaccgt ctccacggct ccgaacacct tctcggccag tgacaccccc
     6661 ttgaccttct gggggttcag ctcgtcaggc gtcaggtgtg gcacgtctcg gcctgcctgc
     6721 tggatcgccc catagtgggt tttaactcgc tggtgtgtgg ccctgctgcc ctcgatgccc
     6781 cgttcaagcc ccagatcggc cacccgttcg gcgtagctgg tctggtcctg gctcatcttc
     6841 gtccggttgc caatgaactc cttggcactc aggcgcccgt cagccgtccg aggcaccacg
     6901 aacgccgaca ggtgcggggt cagctcatcc cggtggatcg tggccgtgac cacgttctca
     6961 gcgccgtact tgtcggccag ccactcggtc gatcgcttga agaactcccg ctgctggtcg
     7021 gcgcttgcgg tctgccacca ctccggcgag gcgctcatca cgtattcgac cgccagcacc
     7081 gcatccttgc gccgcttctc cggcaaccgc tccctgagct tgcccatcgc ctcatcggtc
     7141 gatctggccg ccaggtgatc gttgtccggt gtgcgctcct gatcggcgtt cggcgtctcg
     7201 cggtcgcggt agcagtgctg caacgcagcc gccaccgtgc ccatgctgtt gagcttcttc
     7261 gccctcatga tcgcaaacgc catccgtccc cctccgcact gtcagattgc tgccagcccg
     7321 tcgaggtacg aatgtgcctc cggcgggcca cttctttgaa gtgtaaactc actagacttt
     7381 actccgtaaa gtacgtgacc gctacgcggt tgcagcggcc tgcggccttg ctctacgggc
     7441 ttcgccctgc gcctcccttc ggtcgttgcg aacaccttgg ggcgctgccc caaaccccgc
     7501 gttcgccacg tggcaggggt gcaggtggcc gcgccgcttc gctctgctct acctgccccc
     7561 ttgcctgagc gtgggggtca ctccatcacg tcaagggttc gcttcgccgg gtgctcaccc
     7621 gatcccttcc gcttcgctcc agggctgcgg cttccgctcc cgcccttgac ctgattccgt
     7681 tccgagtgga ggcggggtca aggggtcgtg gaataccgca aggcgcgcag cgcctgagga
     7741 tatgccgcga aagccccttg acgctgccgg aacgacccag cctctggctc tggggttggg
     7801 aacgaaggga gcgcagcgac cgcaggcccc agcccccgag cctcacggcg gcgagtgtgg
     7861 ggagtccaga ggggcaaagc ccctttgggc ctagcgcagc ggaaacaaac cctctcgcct
     7921 ctggcgaggc attaactcca agggagcgca gcgaccgtca gcagggtgcc aagcgaagcg
     7981 ccggcgggac ggcagtccct tagaggagaa ggggaaaaat actaaatgat tgatttatat
     8041 agtttttaaa ttttgtgata ggaaacgtag ggttccggac aaccggaagc agcttgtttc
     8101 cgcttacttc ctatcatagg aaacaaattg cttgacttgg gaaacggttt tgggaagtaa
     8161 ttttcctatg gtaggaatta aacgtccggt taagcggacg ccctgtttcc ggttaggagg
     8221 tcaacaatgc aaagcgtcac cgtagcgaca gagacacaga gacgaatcag gcaaagggag
     8281 ctggcagcag cagacgagaa ggaggctcag gagcgcgagg aggcgcgaaa gaacaagggg
     8341 ttcacacagg tatattcaaa gggatggcgg cgcgtcaggg agggggtctg aagtccaatg
     8401 gaacgaaaac gtacgttagt gaagtaactg tatgatatat caagacataa tggacataga
     8461 aaaacgccat caaaacggtg tctttttaat cgaaaattgg cacttaacgg acggcattgt
     8521 ctactaatcg atttttagac gataattttc ctcttaccgt acattttcgt acggttggcc
     8581 ctcaaacccc gatttgaact caatcggata acctgaatgc aataccgcaa tggcgataat
     8641 ttaaaagcga tagccgttga cgcaaaatca atccgttacg catctctatt ggttaaataa
     8701 tgcgcttaac gtacaaaatt ttcccatatc caaactgacc ccttaaagga catcaacacg
     8761 cattcggcgt tttttcttgt ccaattgtga ttttcgtgag ctccgtatca gtgaaatttc
     8821 cctttcctga ttctgtgtca aagtcaccag gtttgaaccg gtgtacatcc tgccatatag
     8881 aaaaacaggt gtggcaggtg acaggcagtt ctccatcagc caccttttcg cagttttcaa
     8941 tcagtacccg ttccttattg tattgtaagt tataaccact tgtgcaaagc gtttcatacc
     9001 cgtgagcgcc tgtcacgata tgtgtggcaa tccgttccct gacttttccg ttcactaata
     9061 actgttcacg tgtggtgatc gttcgcattc tcttcctcat cagctttcct gtagaagcct
     9121 cctgactata aggctccgga tatcttaccc gctcattttg gctggaggta gtggtggcgt
     9181 cagaggatga attactgaca ttgtatatga agctttgagt tttttctgga tttcatcagt
     9241 gtttatcttt atttgttctt gtttgttctc ttttgtgctg ttggagtcgg tttttgtctg
     9301 tatttttctt tatttttcca tttgtggccg atatcaggtc tttaccgata cgttgtttta
     9361 ggtgataatc gtttttgatc tcgtattttt ctaaaaaaat ccattttatt tatgtgaggt
     9421 tctagatgcg tatctttgta gacgacggct ctaccaacat caaactggcc tgggttgaag
     9481 acggtgcagt gaaaacgtta attagtccga atagttttaa gccggaatgg tctatgacgc
     9541 tgcagggact gactgatgcc actgtacccg cttccgcaaa ctatgagatt gatggagaaa
     9601 aatacagctt tgatcaattg agtccggatg cagtgcgcac cacagaaacg cgttatcagt
     9661 acagcgacgt taatgtggtt gctattcatc atgcgttgat gcagtcaggt attgctccac
     9721 aacctgttga tattgttgtg actttacctt taggtgagta tctggatgag aacgatcagc
     9781 caaatctggc taatatcgaa cgtaagaaaa ataatgtccg gcgaactgta acagttcagg
     9841 ggcgtgaaaa ctttaccatt cgtaaagtca gtgtcttgcc tgagtccgtg cctgccggtt
     9901 ttgacgtgct gaaagatctg aatgatctgg agtcgctgtt gattgttgat atcggtggaa
     9961 cgacccttga tattgcacat gtccgcagca aaatgtcagg tttcacaaaa acctattgtg
    10021 accccaagac tggcgtctcc atcattactg aagcagttaa gcatgcgatg gatgtgaatg
    10081 tgagtacacg aaccagttct tattttgcag atcgtctaat ccagggccgg gatgacaatg
    10141 aattcctgac ccgttatatc ccgaatgcag aacagcgtga gcggatcctg acagtattac
    10201 gtgaccggga aaaaaaactg cagcatcgtg tggcggattc agtaggacgc tttgctggtt
    10261 ttactcatgt tatggttgtt ggcggtggtg ccagccttgt ggctggggca gtaaaaaaag
    10321 cgacaggagt gggtgataac cgtttctttg tcagtgacaa tcctcagttc gatctggtgt
    10381 tgggaatgat ggcaatgaag ggatgagtat ggctgataat cgtaagtgct ctttttatat
    10441 ttatccggaa cgaaatgcag ctgatcgggt ggctgacagg tttcttgaaa aattgcctca
    10501 gaaagaacgt ggtcgggcaa tgcgggccat gatgttgtgt ggtgctgcat tgatgaagca
    10561 ggatgaacga ttgccgttcc tgatagctga attcctgaca gacagcacgt ctatgcagga
    10621 tattcaacga attatcagta gtacgctacc acaacaggaa aatggtgaag ttgttcgttt
    10681 gctggaggca tttctgcagt ctgccggtaa taatgcaaaa acgatcctgc ctgcggttga
    10741 ttctgctacg caagaaatat ctgctccagt cgatcagaat ctcctggaaa caagaaacaa
    10801 cattaagaac ttgtttccgg atgatgaata agaactgaac tctgttaaag gacgctgttg
    10861 cgtccttttt tcttttccga caaagaatgt cgctgggctc ctctttttac tgcgccggct
    10921 gacgcggcgc ggcaggaacg ctgcctgtgg tcagtgtccc gcgtccggcg ggcacgggac
    10981 gggcgctttt accgctcccg gctggcgtcg gtgacagtgt acgccagccc gtcgcccttt
    11041 tctgatgaac gcccttcaag ccgctttcgc ggcatagcct tgccgtcaaa aagacgacgg
    11101 ctgcggtatt ccacggtcgg cctgacccgt taccggacgc ggtgaacagc ccacaggcag
    11161 cggggaacgg gcgctacagg ggcgccctcc cggtgctctc tgctgaagca gaagtgagag
    11221 gagaattccc atgccaaact ggtgcagtaa ccgtatgtat ttttctggtg aaccggcaca
    11281 gatcgctgag attaaacgac tggccagcgg tgcagtcaca ccgctttatc gccgcgccac
    11341 aaatgaaggt attcagctgt ttctggccgg aagtgccgga cttctgcaga tcactgaaaa
    11401 tatccgctct gagcagtgcc ccggagtgac ggctgccgga cgtggtgctg tatcactgga
    11461 aaatatcgcg ttcacccgct ggctgacaca cctgcagaac ggtgtactgc tggatgagca
    11521 aaactgcctg atgctgcatg aactctggct gcagagcggt actggacagc gtcgctggga
    11581 agggttaccg gatgatgtca gggaaactat cactgtgcat ttcaccgcaa aaagaggtga
    11641 ctggtgtgac atctggggta acgaggatgt atcggtgtgg tggaaccgtc tgtgtgacaa
    11701 cgtactgccg gaaaaaacca tgccgtttga cctgctgacg gttttgccga cccgcctgga
    11761 tattgaagtg aatggcttta acggcggtgt tctgaacggt gttccttctg catatcactg
    11821 gtatacagaa cggtatggcg tgaagtggcc ttgtgggtat gacctgaata tcagcagtca
    11881 gggggaaaac ttcattcagg tggatttcga cacgccgtgg tgtcagccgg aaagcgacgt
    11941 tgttgcagaa ttaagccgcc gtttcggctg cacgctggag cactggtacg ccgaacaggg
    12001 ctgcaatttc tgtggctggc agttgtatga gcgcggagag cttgttgatg tgctgtgggg
    12061 ggaactggaa tggtcttccc cgacagatga cgatgagctg ccggaagtca ccggacctgc
    12121 gtggatagtc gacaatgtga cgcattatgg cggatgaagt atgacggaga cgggcgggca
    12181 accgcccgtt tcttttccga caaaggatgt cgccgtgctc ctctttttac tgcgccggct
    12241 gacgcggcgc ggcataaacg ctgcctgtgg tcagtgtccc gcgtccggcg ggcacgggac
    12301 gggcgctttt accgctcccg gctggcgtcg gtgacagtgt acgccagcct gtcgcccttt
    12361 tctgatgaac gcccttcaag ccgctttcgc ggcatagcct tgccgtcaga aagacgacgg
    12421 ctgcggtatt ccacggtcgg cctgacccgt taccggacgc ggtgaacagc ccacaggcag
    12481 cggggaacgg gcaccacagg ggtgccctcc cggtgtcctc tgtaagagaa ggagcgttgt
    12541 tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg gcccgtattc ctgataacgc
    12601 cattgatttc atactgactg acccgccgta cctcgtcggt tttcgtgacc gtttcgggcg
    12661 caccatcgct ggcgataaaa ccgatgaatg gctgcaaccg gcctgtaatg aaatgtaccg
    12721 cgtactcaaa aaagacgcgc tgatggtgag cttctacggc tggaaccgcg tcgatcgctt
    12781 tatggccgcc tggaaaaatg cgggattcgg cgttgttggt cacctggtct tcaccaaaaa
    12841 ctacacatcg aaggccgcat atgtgggcta tcgccacgaa tgcgcctaca tcctggcaaa
    12901 aggacgtccg gcgctgcctc agaagccatt gcccgacgtg ctgggctgga aatattcagg
    12961 caatcgtcat cacccgactg aaaagcccgt taccagcctg caaccgctca ttgagagctt
    13021 cacacatccg aacgcgattg tgctggaccc gtttgcaggc agcggatcaa cctgcgtggc
    13081 cgctcttcag tccggacgcc ggtatatagg tatcgagctg cttgaacagt atcaccgtgc
    13141 cggacagcaa cgactgaccg ccgtgcaacg ggccatgcag cagggggccg cgaatgataa
    13201 ctggtttgaa ccggaggctg cgtaaatgaa ctatgccgga cacgaaaaac ttcgcgccga
    13261 cgtggcggag gtggccaaca ccatgtgtga ccttcgggcc aggctgaacg atatggagca
    13321 tcgctgtcgc tttgattccg atgtgcttgt tgaacgcctg acacgccaga cgctgtatcg
    13381 cgccaatcgc ctgtttatgg aggcgtatac cgaaattctg gaactggatt cctgcttcaa
    13441 agattgagaa ggagacgaga atgtacggaa catgcgaaac gctctgccgt gagctggcag
    13501 cgcagtatac ggagaacacg ccgctgatgc tggttgtctg gtccccggaa gagattcagg
    13561 cactggctga cggtatggaa atttctctga ccggtcatga aatcaggacc gtcctggcac
    13621 gcctggagga catcccggaa gaacagcgga ttgagtccgg tatttcttcc gctgcggtga
    13681 tggagatcat caataacgtg agcgaaaacc gccaggtgac cgtccctgct gaactgctgg
    13741 cgtccctgat tcagaccgct gagcaggcat tgtggaaacg tgaatgggcc gcccgggatc
    13801 atggcctcgc cgtcccggaa tgcgtcaccc gccgtcagga ggtggttaat caggcccgca
    13861 ccctgctgaa aaacaacaca cacgaaaacg actgatgtta tcgccgcctc cgggcggcga
    13921 tacagggagt tcatgaatta tgaatgaaac gctgaatgca ctgatttatc gccacgccag
    13981 caacctgttg ctggcgcagg gctggccgga agaaacggat gttgaccagc gtaacccgaa
    14041 gtatccgggc tgggtcagca tttacgtgcg gctggatgcg ccccggctgg cgacgttact
    14101 tatcaaccgt cacggcggcg tactgccgcc gctcctggcc tccgccattc acaaactgac
    14161 cggaaccgga gcggaacttg ttctgtccgg cagtcagtgg cagtcgctgc cggtacttcc
    14221 ggcagacgga acgcaggtgt ctttcccgta tgccggtgag tggctgaccg aagacgaaat
    14281 cagggccgtt cttgatgcgg tacgcgatgc agtatgcagt gtcagttacc aggtggcaga
    14341 agatgcgcgg cgtatccgtg cggcgctgac caccaccggt cagacgttgc tgacccgcca
    14401 gacgcgccgc tttcgcctgg tggtgaagga aagcgatcac ccctgctggc tcgatgaata
    14461 cgacgaaaac ctgcccgtgg tgctcgatgc catcctgaac cggggcgctc gtttttcgtc
    14521 ggtggaaatg tatctggtca gtgagtgtgt ggagcatatc ctgtccagtg ggctggcctg
    14581 cgatgtgctg cgtataccgg atgaaccgcc ccgccgctgg tttgaccgtg gtgttctgcg
    14641 ggaggtggtc ctggaagccc ggagtgaaat ccgcagcatg gcggatgcac tggcaaaaat
    14701 caggggctga tgatggcggg gaaaatcccc gcctttttcc tgccggtccc cggaaagagc
    14761 aaaaggatgt cgccgcaggc tccgctttac ccggccatgc ggggcatggc cttgtgggtt
    14821 ttcagttatg tggcctcagc gtcgtgtgcg ggctgtgccg tgcctccatc gtagccgggc
    14881 ttctgcgggc gggttgcaag gtaagatgaa ccgctgcggt cacccggtcc ctccttcccg
    14941 tctgccgtga ttttccggtc gttcacccgc tcagatttcg cggtctcacc ctccaactcc
    15001 cgccagccgt gtgcgtcagg ctgcggcttc ccttgcgccc ttgcaaccct gcaggcccgg
    15061 caaagatgga ggcacggcac cgcccggtgt cgcggaatat gtgaacaacg gaggaaaaca
    15121 tcatgcaata tgcgaaacct gtcactctga atgttgaaga gtgcgaccgt ctctcctttc
    15181 tgccttacct gtttggcctg gattttctgt atgccgaggc atctgtgtac gcgctggcga
    15241 aaaaaatgat gccggaatat gaaggcggat tctggcactt catccgcctg ccggacggtg
    15301 gcggttacat gatgccggat ggcgaccgtt ttcatctggt caacggtgaa aactggtttg
    15361 accgtaccgt gagtgctgat gccgcaggca tcattttgac ttcccttgtg attaaccgcc
    15421 agttgtggct gtaccacgac agcggggatg caggactgac ccatctttac cggatgcgcg
    15481 atgcgcagtt gtggcgtcac atcgaatttc accccgaatg caacgcgatt tacgcggcgc
    15541 tggactgatt aacggacggg gcggcaatgc cgcccctgta aaggagacga gaacatgtac
    15601 tgtactgtta aagaaattat ccgcgaagtg ctggatacag acgtgccgga cagtgaatgc
    15661 gtttttgccg tggtgctgac tcgtggggat gtgcgtcaca tagcccagga ctggagtctg
    15721 acagacgatg agctggaaac cgtcatgcag cggctggacg atgcctttga gcatggtgcg
    15781 gatgtcagcg ttgttcacga cgttgttcgt gaactgatgg aggaaaagca cgccagccgt
    15841 caggtgacag tcccggcggt gatgctggaa aaagtgatgg cgctggcagg cagtgaaatg
    15901 aagcgcctgt atgccgtcgg gagtgagaac gggggcgacg gtgatgcgtt cgtcagggaa
    15961 gaacgcgaag caatggacgt tgtgttacag gcgctggacg gggagcacat gtcatgaata
    16021 tcagcacaga aacccgcgaa attctgcgta attacagggc cgtgattaat gcgcggcgtc
    16081 gtgaaatggg gcagaaaccg ctcaccaccg cgcagattat tgatgaaatc tgcgattttg
    16141 tggcgaatca gcaggcggtt ttcctgggcg gtcactacat cttacagggc agcagaaaca
    16201 ggtgatacag gggcaggcgg tggaggccgc ctgtcagccc gcgcgaaaac ccctggcagg
    16261 ctgacgcctg ccggaaggga agcgggccac gctggcgcgt cgcccgttac ccttcccgtc
    16321 tgtgatgtgt ctgactcagg agtgtttctt tcttatgtta tggaactgtt cctgatgttg
    16381 ttgtcgttgt tcctttatct gctgctctga ttctgactac tgcagtgttg ttctgtctgc
    16441 cggacgcacc ttcggctccc ggctgcctgg cggcatccgg tcgtcaggcc cgactccgac
    16501 aggcagctgc tggcgcaccc gccagtctcc gccgaacccg ctggcgcggt ttcgggctgt
    16561 cgctacggtc cgatgcgtga aaacccccgg cttgcagcga ccgttccggt cgcgccgcct
    16621 ctgtcgcgcc gtggcctgcg ctcccggcat cccggcaaaa ggcctccggg atgtaaggcg
    16681 caacgccgtt tcgcgcacgc gctcttcgtc gttgcagcgg gcccctgctc attgcctgcc
    16741 gggtgccaca cacccgtatg agtccctgtc gccgggggct gatacagcca caacctgccg
    16801 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa
    16861 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg
    16921 aaggttctca aacgggtgaa gttcatacca cagggaatac agcagcaggt tcccgcgaaa
    16981 cgtcaaaacc gcggctcccg aaactcaacg ggtgatgttc tttaaaaatc aggatttatt
    17041 tcagactccg gcaatacgca tttatgtgtt ctgtcagcat cctgctggca ggtaatgaga
    17101 acgcaggaac agaaggagga atacaccgga gaacgtcaga cgcagatatg aaaaaagcag
    17161 gccggggagg acctgcttaa tccacaccgc aaaaactgtt cgagcagttt ttgcggctcc
    17221 agtcaatcgt cataagaggc taaattatga caataagtat tgtttcacct tccgcagcgg
    17281 ctgtaaagcc ccgccgtcat ccccgtttca gacgcgaaga catccccgcg ccggaaattg
    17341 accccgtgct gaaagccttc gggcgacaca tagcccggag ttttcaccgg gggcgtggtg
    17401 tacacatccc cgccatgaaa aacacggcct tcgggcaggt gctccgcaca ctcgaactga
    17461 aacgcgcttt taactgatga ggtgagccgc cccgcgaggg gcggcaacag acggaaggag
    17521 aacattttat gacattagca gcaatcacca tgaccgcccc ggaagccgcc agccctgtgc
    17581 agatgtaccg cgcgacctac tcaccggatg acaacaaact gcgcctgtat gccgcgtcac
    17641 gtcttgaccc ggagacgtat aaaaaagtgc atgatgccgg ttttcgctgg gcacccagac
    17701 aggcgctgtt tgtcgcgcca gcctggacac cgggccggga agacgtgctc ctctcacttg
    17761 ccggagagat tgaggatgaa gacagcacgc tcgctgaacg tcaggaagca cgggcggagc
    17821 ggtttaccgg atacagcgga aagcgggcca gtgaatccgc acaggcactt gatgaagtgg
    17881 aaagactggc cgcgatgatc ccgcccggtc agcccattct tgtggggcat cacagcgaac
    17941 gccgcgcccg tcgtgatgca cagcgtattg aaaacggtat gaaacgtgct gtgatgctct
    18001 ttgaacgtgc ggaatactgg gaagagcggg cgcggtcggc actgcttcac gcgaagtata
    18061 aagaacgtcc ggacgttcgc tggcgtcgta tcaaaaaaat cgaagctgat ttgcgcaagg
    18121 cggaaaagac catcgcacag tcgcagaaat atctgacgat gtggcgggct gaatcgctgg
    18181 atctgaatat ggcaaaactc atcagcagtc atgaccatat cagcgcctgt ttcccgctgg
    18241 acaggtatcc gcgcccggca gaaaaaagcc agtatgaagg aagtcgatcg ttatggtcgg
    18301 ccctggatga tgacatcatc accacggagc aggcccgcga aattgcgatc cgctgtcatg
    18361 aacggcagat tcagcatcag caacgctggg ttaaccacta tcagaaccgc ctgatctatg
    18421 agcgtgccat gctggacgaa agcggcggcg tggttacccg gacacaggat tttgagccgg
    18481 gcggacaggt tttcagccgg ggcgagtggc tgaccatcat ccgcgtgaac aaaagcaacg
    18541 gggcggtgag ttcagtcaca acgccgaatt acagttttct cgggtacagc ggcacgatga
    18601 aagtgacgcc cgatcgcatc acggactaca aagcaccatc ggcagaagag gctgccgtcg
    18661 ccagtcaggc cgcgaagcgt ccgccggtag tcaactatcc gggggacggt ttccgggaaa
    18721 tgacaaaggc gcagtgggcc gccctgcccc gggactgtaa ggccgtgcgc agtgtggcag
    18781 agacagaaga ccacggggca taccgctacc gccgcacaat ggacaataat ttccgcctgg
    18841 tgaatgtgta tattaccgac atgaaaatca cggaaatccc acagaaataa ggtatatccc
    18901 cccgggaaat ccccggggga tgacggagat aatgacatgc acagtcagtt aagagaacgt
    18961 atccggctga tgcgcgcaag gctggataac gccgcgccgg ttgctgaaat ccgggctgaa
    19021 tctcagcttt ttgtgactcc tgccccggtc tgtgatcgcc tggtgacgct ggcggaaatc
    19081 agcaaccgtg atcacattct ggaaccctct gccggcaccg gggccatact gcgggcgatt
    19141 cgggataccg cgccggaggc catgtgtgat gcggtggaga ttaacagcgg gctggtccgg
    19201 tatctgcggg aaaattttaa cggtgtcagg gtacagtgtg gcgacttcat ggaatggcag
    19261 ccggtgcagt attacagccg gatcattatg aatccgccgt tcatccacgg gcaggatatc
    19321 cggcatatcc tgcgggcctt ttccctgttg cgtccggggg gcgtgctggt tgccgtctgt
    19381 ctgaacgggc cgcgccagca ggagaagctg ttaccgtttt ctgacatccg cgaggagctg
    19441 ccgcgcggca cgtttgctta taccgatgtc ccgacgatga ttattcgttt gcgtgcctga
    19501 tgacacaggg ctggcgggag gataaatgcc cgtctgtccg cgccgggaaa agacagcacg
    19561 ccggtggcgc tgaaaggggc cgcgcttacg cgccgcccct ttcattacgc ggatgttctg
    19621 ctgtgtgctt ctgtcttttc atgcctgtca tttgtctgtt actgatgatg ttgttgttcc
    19681 tgctactgct actgctactg ctactgctac tgct
//
LOCUS       DAZFKM010000092        19613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFKM010000092 DAZFKM010000000
VERSION     DAZFKM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..975)
                     /gene="melR"
                     /locus_tag="ACHKAY_003365"
     CDS             complement(67..975)
                     /gene="melR"
                     /locus_tag="ACHKAY_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO5701988.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1258..2613
                     /gene="melA"
                     /locus_tag="ACHKAY_003366"
     CDS             1258..2613
                     /gene="melA"
                     /locus_tag="ACHKAY_003366"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO5701989.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2716..4137
                     /gene="melB"
                     /locus_tag="ACHKAY_003367"
     CDS             2716..4137
                     /gene="melB"
                     /locus_tag="ACHKAY_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO5701990.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4276..4905)
                     /locus_tag="ACHKAY_003368"
     CDS             complement(4276..4905)
                     /locus_tag="ACHKAY_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO5701991.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5028..6674)
                     /gene="fumB"
                     /locus_tag="ACHKAY_003369"
     CDS             complement(5028..6674)
                     /gene="fumB"
                     /locus_tag="ACHKAY_003369"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO5701992.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6752..8092)
                     /gene="dcuB"
                     /locus_tag="ACHKAY_003370"
     CDS             complement(6752..8092)
                     /gene="dcuB"
                     /locus_tag="ACHKAY_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO5701993.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8663..9382)
                     /gene="dcuR"
                     /locus_tag="ACHKAY_003371"
     CDS             complement(8663..9382)
                     /gene="dcuR"
                     /locus_tag="ACHKAY_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO5701994.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9379..11010)
                     /locus_tag="ACHKAY_003372"
     CDS             complement(9379..11010)
                     /locus_tag="ACHKAY_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO5701995.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11191..11421
                     /gene="yjdI"
                     /locus_tag="ACHKAY_003373"
     CDS             11191..11421
                     /gene="yjdI"
                     /locus_tag="ACHKAY_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO5701996.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            11433..11705
                     /locus_tag="ACHKAY_003374"
     CDS             11433..11705
                     /locus_tag="ACHKAY_003374"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5701997.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11932..12228
                     /gene="ghoS"
                     /locus_tag="ACHKAY_003375"
     CDS             11932..12228
                     /gene="ghoS"
                     /locus_tag="ACHKAY_003375"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5701998.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12256..12429
                     /gene="ghoT"
                     /locus_tag="ACHKAY_003376"
     CDS             12256..12429
                     /gene="ghoT"
                     /locus_tag="ACHKAY_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO5701999.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12548..14065)
                     /gene="lysS"
                     /locus_tag="ACHKAY_003377"
     CDS             complement(12548..14065)
                     /gene="lysS"
                     /locus_tag="ACHKAY_003377"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO5702000.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14302..15759)
                     /gene="dtpC"
                     /locus_tag="ACHKAY_003378"
     CDS             complement(14302..15759)
                     /gene="dtpC"
                     /locus_tag="ACHKAY_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO5702001.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15818..17965)
                     /gene="cadA"
                     /locus_tag="ACHKAY_003379"
     CDS             complement(15818..17965)
                     /gene="cadA"
                     /locus_tag="ACHKAY_003379"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO5702002.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKELKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18045..19379)
                     /gene="cadB"
                     /locus_tag="ACHKAY_003380"
     CDS             complement(18045..19379)
                     /gene="cadB"
                     /locus_tag="ACHKAY_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO5702003.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         5025 a         4688 c         4860 g         5040 t
ORIGIN      
        1 ttaaattatt ttcactgctt attatcgacg gctaaactat ttttttggct gatactgata
       61 tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg
      121 cgacatgccg acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc
      181 cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt
      241 gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc
      301 cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg
      361 atcatagttt tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga
      421 gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agccagagag
      481 gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcgtt
      541 cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt gctgtgtggc
      601 cagtgatttg atcaccatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca
      661 ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctcttacagg ttccggtatc
      721 tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac
      781 tttttcattg ttgatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg
      841 accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg
      901 gtattccgaa tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa
      961 cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg caggagtgag
     1021 ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccagagagg cgaggggagc
     1081 gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattacc ataaactcag
     1141 atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag
     1201 gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg
     1261 atgactgcac ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt
     1321 cttggtgatg tgttccatcg cgaggcgctg aaaacggcgc atattgccct gatggacatt
     1381 gatcccaccc gcctggaaga gtcgcatatt gtggtgcgta agcttatgga ttcagcaggg
     1441 gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacatga tgccgatttt
     1501 gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc
     1561 tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg
     1621 cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac ggaagtctgc
     1681 cccgatgcca ccatgctcaa ctatgttaac ccaatggcga tgaatacctg ggcgatgtat
     1741 gcccgctatc cgcatatcaa acaggtcggg ctgtgccatt cggtgcaggg aacggcggaa
     1801 gagctggcgc gtgacctcaa tatcgaccca gctacgctgc gttaccgttg cgcaggtatc
     1861 aaccatatgg cgttttacct ggagctggag cgcaaaaccg ccgacggcag ttatgtgaat
     1921 ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc gaatattcat
     1981 ggcaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc
     2041 gtcacggaat cgtcagaaca ttttgctgag tacacaccgt ggtttattaa gccaggtcgt
     2101 gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag
     2161 cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt
     2221 aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagccgagt
     2281 gtgatttacg gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc
     2341 gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta
     2401 ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa
     2461 gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact
     2521 gctgccgtgc tgggcattga tgaaatatat gctcttgttg acgacctgat tgccgcccac
     2581 ggcgactggc tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg
     2641 ggatttattc cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca
     2701 gcgacccgat accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg
     2761 ttcgggaagg attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat
     2821 gtcgttgggc tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat
     2881 gctattaacg atccgattat gggatggatt gtaaatgcta cgcgatcgcg atggggtaag
     2941 ttcaaaccct ggatcctgat cggtacgttg gcaaactctg taatcttatt tctcctcttt
     3001 agtgcgcatc tgtttgaagg tactactcag attgtctttg tttgcgtgac ctacatcctc
     3061 tggggcatga cttacaccat tatggatatt cccttctggt cgctggttcc aaccatcacg
     3121 ctcgataaac gtgagcgcga acaactggtt ccttatccgc gtttttttgc cagtctggca
     3181 ggctttgtta cggcaggtgt gacgctacca tttgttaatt atgtcggcgg tggcgatcgg
     3241 ggatttggct ttcagatgtt cactctggta ctaatcgcct tttttattgt ttcaaccatc
     3301 atcactctgc gcaatgtgca tgaagtcttt tcgtcagaca atcaaccgtc tgctgaagga
     3361 agccatctga cacttaaagc catcgttgcg ctaatttata aaaacgatca gctttcatgc
     3421 ctcttgggta tggctcttgc ttataatgta gccagcaaca ttattaccgg atttgctatc
     3481 tattatttct catatgttat cggtgatgcg gatttgttcc cctattatct gtcgtatgcg
     3541 ggagctgcta acctggtgac gttagtattc ttcccacgct tagttaaatc attatcccga
     3601 cgcattttat gggccggagc atctattctt ccggtgttaa gctgtggtgt tctcctgtta
     3661 atggcattaa tgagctatca caacgtcgtc ctcattgtga ttgcgggtat tttgctgaat
     3721 gtgggaacgg cgcttttctg ggtattacag gtcatcatgg tggcagatac cgttgattac
     3781 ggtgaatata aactgcacgt acgctgtgaa agtatcgctt actccgtgca gactatggtg
     3841 gtgaagggcg gttcagcctt tgcggctttt ttcattgcgg ttgtgttagg gatgattggc
     3901 tatgtaccga atgttgaaca gtctacgcaa gccctattag gtatgcagtt tattatgatt
     3961 gctctaccaa ctctgttttt catggtaacg ctgattctct acttccgttt ctatcgcctc
     4021 aatggcgaca cgctgcgcag gatccagatt catctgctgg ataaatatcg caaagtaccg
     4081 cccgagcctg ttcatgctga tattccggtc ggtgcagtga gtgatgtgaa agcctgacgt
     4141 ggacaatagc taacgaaaat ggcctgatgc gatatgttta tcaggccacg tcttaatgta
     4201 atgctttgga tttgcaaatt ttgtagaccg gataaggaat tcacgccgca tccggcatca
     4261 acaaagcgca cgttgttatc cggttatcaa gccaaagcgc cgtagctggc ggcaatgaaa
     4321 gcgagcgagg aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca tatcggattg
     4381 cgtgtcccac tggtcgccct gggtgccgag aaaatcatcc gctccctggc ccattgccag
     4441 cgccgcccac cactcgatta attcatacat cgcgcttatc gccagcgcca cgcagcagac
     4501 caggaacgcc accattttac gtccgcgcac gtacatcccg cgcacgagaa tttctcgtgc
     4561 caccagtgca ggcaccagcc cctggaaaaa atgccccagc ttgtcatacg gattacggct
     4621 caaccccagc cattcctgca cctcaaaacc gatggggact ttcgcgtagg tgtattgccc
     4681 gccgaccatc aggatgatgg cgtgaaagaa aatgagcgta tagagcagcg gcgttaacgg
     4741 ataacgttta gcggtggcaa gcagtagcgg cacgacaata atcaccggtg tcacttccat
     4801 cagccaggtg agtttgtcat gggccgaaat gccggtataa atcaggatta acgttagcgc
     4861 cagtgcgccg gtgttaagaa ttaacggctt gagtgtacgg gtcatggtga gttcgcaagt
     4921 cagggaaaat actgactatt caccagcaag cgtgaaatta caatcgcaaa agaggtggcc
     4981 cagggggatc acctggcagc atgctgccag gcgctgggcc gaagaggtta cttagtgcag
     5041 ttcgcgcact gtttgttgac gatttgctgg aagaagtcgt tacctttgtc atcgaccagg
     5101 ataaacgccg ggaaatcttc tacttcgatt ttccagatag cttccatacc cagctccgga
     5161 taagcgacgc actccagatg cttgatgctc tgctgcgcca gtaccgccgc cggaccgccg
     5221 atgctaccga ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg ctgactgcgg
     5281 ttacctttcg ccagcatgat catgctgccg ccgtgggatt gcagcagatc cacgtaggag
     5341 tccatacggc ctgcggtggt tgggccaagt gaacctgatg gataaccggc aggggttttc
     5401 gccggacccg cgtagtagat cgggtgatct ttgatgtact gcggaagttc tttaccggcg
     5461 tcaatcagct ctttcagctt ggcgtgtgca atatctcggc ccacgataat ggtgccggtg
     5521 agcgacaaac gagtggatac cgggtattgc gaaagctggg cgaggatctc tttcatcggg
     5581 cggttaaggt caactttcac cgcttcgcct tcaccggcct ggcgcagttc ttgtggaatg
     5641 tactggcctg ggttgtgttc cagtttttcg atccagatac cttcgcggtt gattttcgct
     5701 ttaatgttac ggtcagcgga gcaggagacg cccatgccga ccgggcagga tgcgccgtga
     5761 cgtggcagac ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca
     5821 agaccgagtt tctgggcctc ttccagcagt tcctgttcca gctggacatc gcggaacgcc
     5881 tgaccatgtt cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct tgctaacttg
     5941 acggttttca ggttggtttc cgcagacgta ccgccaatca caaacgcgat atggtacggc
     6001 gggcaggctg cagtaccgag ggtacgcatt ttctcgacga ggaagttttt cagtttgccg
     6061 ggagtcagca gggctttggt ttcctggtag agatacgttt tgttggcaga gccgccgcct
     6121 ttcgcaacgc aaaggaattt gtactcatcg ccatctaccg cgtacaggtc gatttgcgca
     6181 ggcaggttag tgccggtgtt gacctctttg tacatgtcca gcgccgcatt ctgtgaatag
     6241 cgcaggttat cttcgatata ggtgttatag acgcctttcg acagcgcttc ttcatcaccg
     6301 ccaccggtcc acacgcgctg gcctttttta ccgacgatga tcgcggtgcc ggtatcctgg
     6361 caggtcggca gcacgccttt ggcggcgatt tcggagtttc ttaagaattg cagcgccacg
     6421 tacttgtcgt tttcgctggc ttctggatcg tgaagaatag ccgcaacctg tttctggtgt
     6481 gccggacgga gcatgaaaga agcgtcgtga aaggcttgct gcgccagtag ggtcagggct
     6541 tctggttcca ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa
     6601 gtgagtagat agtattcggt attgtctttc cccatcggga aaggtgcctg gtagataaag
     6661 ggtttgtttg acatagcttc cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc
     6721 cgcgtagtca cgggcggcac gcaaagtgca tttataagaa cccgtacatc gcggcgaaga
     6781 tccagccgaa gacgcacgat acgctcacac caatcaaccc cggcagaata aaactgtggt
     6841 tgatgacgaa gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc gctgccagat
     6901 cgctcggata agtcggcagg atgtaataac cgtagcaagc cggtgctgaa gccacgatgt
     6961 atgccggatc aacgccgatc gccagcgcga ccggaacaat cgccgccagc gccgcagcct
     7021 gagagtttac aaacttggaa accagcagca gaacaatggc atacgcccac ggatactctt
     7081 tcaccatttc acccagtacg ccctgaattt cagacatatg cgcaccgaac atggtttctg
     7141 ccatccatgc gataccgtac accgccacga tggcgatcat accggaacgg aagacttcgt
     7201 tttttgagat agacgcggga ttggttttgg tcaggataat aatcagcgcc ccggtcagca
     7261 gcataaacat ctgaataacc agtaccatcg acagcggttt gccgccgaag gatggacgca
     7321 ggtccgaatc agcaccaaga agggcgacta cagcgattgc accgaggaaa atccacattg
     7381 ccagccagtt gcttttcggc agttttttat cgagcagcgt cgcggtatca ccgtaaacat
     7441 actcacggtt ttccggtacg gagatgaatt tctggaactc ttcgtcttta tccagatctt
     7501 taccgcggaa ccagctgaag ataccgatcg ccaggatacc gattaacgtc gatggaatgg
     7561 tgattgccag caggtcgagg aactcaagat ggcgaccatc aaaggtgaca ttacccagca
     7621 ttgcaaccag agacacgacc gcaaccgaca ccggactggc gataatcccc atctgtgcac
     7681 cgatagaact tgccgccatc ggacgttccg gacggatgtt gttcttaatg gcgacgtcgt
     7741 agatgatcgg cagaatggtg taaaccacat gacccgtacc gcaaagaatg gtcagtgtac
     7801 aggtcacaaa cggcgcgaca attgagacat atttcgggtt gcggcgcagc agcttctcgg
     7861 caatttgcag catgacatca agaccgcccg aagcttgcaa ggtcgccgat gccgccacca
     7921 ccgcaatgat aaccagcatg acatcaaccg gtggtttacc tggctgaagg tggaagacga
     7981 agaccagaat gaccagaccg ataccgccta ataaacccag cgcgatacca ccctttctgg
     8041 caccataaaa cagacatatc agtattatga taagttggat agtaaataac atgtgtgaac
     8101 cctcgcgata atcctattta aatttttgct gaatagatca cagtcacgtt ctgttttgta
     8161 tgaactgttt tcagagcctg actaaatatc cgtttggtcg ttaccggctt tagcaaatac
     8221 ctcacagtaa atattggctg attaaagcag cggcaaattt atgcactgtg ataagcggct
     8281 ttttcagaga gaaaaactct ctggcgatgg gtttatagtt tgtactgcgc agatgttaat
     8341 gttttgtttg ctgttatctt attgatattt atcggtctat tttttgttct cagggcagat
     8401 atcattaggt ttaaagatta atctgatcta cccatttgtg ggtaaaaata cacataatgc
     8461 gggtgacata atagttaatt aacttttgtt atcgttttga aattaaaaac accgttcacc
     8521 tgaagagata ttaattttta gcgatgatgg agggataatt atatttgatc tggcacaagt
     8581 tttactgatg aaggatgtaa cttgtgccag gggtattttg cattacggta ataattatta
     8641 cttacagata accagcgatc cgttattggc aatattgttt cagtagtgag tagtgttctg
     8701 cctgaatacg gtaacggtaa accggacgcc ccgtgacgcc ataatggata ctggtgaaca
     8761 agatgtggca gttgaccagc cagatgaggt atttacggca ggaaacacgc gagatgttaa
     8821 cctcgttagc cagctcgtcg gttgaaaact catagtcctg atgcgcgtca atccactggc
     8881 acagcgtgcg taaagtctgc ggcgttaagc cttttggcaa gcgacgagga tcctgttcgt
     8941 tggagctgct gccgtggatt agctgatcaa gctcggcctg gtcataatac tgatgttttt
     9001 ccagcgccat tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg gaagcctgga
     9061 agggtttgat caggtaatcc acgacaccgt aatgcagcga atctttaatg gttgccgcat
     9121 cggctgcgga ggagatgaca atcacatcac ttttgcaacg cgcgttatgc aggacaggca
     9181 gtaaatcgag cccgttctct ttttgcatat agatatcgag caatatcagg tcgataggcg
     9241 tatcgctatt gaagataatc tctttggctt tctccagcgt cgaggctgtt ccacagcatt
     9301 gaaagcctgg gatttgtgct acgtatcggc gattcagctc cgcgaccatt gcgtcgtcat
     9361 cgataattaa tacattgatc atctgttcga cctctccccg tcccagggta tctggacaaa
     9421 aaattgtgtg aaaatcccgg gttccgattc cacggcgatg ctgccgccga gattttctac
     9481 ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt
     9541 gtcaaaaatg tgatcgattt tgtcgggtgc gattcccggt ccatcatcgt taacttcgca
     9601 gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg gttccggccc
     9661 taatgcttcc agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg tcgcgacctg
     9721 gtcctcactg ccgctgtctg gcagctggct ttcactgttt aaaatcagcg tatggcctaa
     9781 atcggtcgcg cggttaatct tgctgattaa aaaaccagcg ataaccggag atttgatctt
     9841 acccagcaga gagccaatct cttcctgata gttattggct gttttgagaa tgtaatcttc
     9901 caactgctta taactcttca gatgcaataa tccgagaatc acatgcaatt tattcataaa
     9961 ttcgtgggat cgttcacgaa gtgcatcagc atagttgacc agaccgtcga gtcgctgcat
    10021 cagtttacgt acttcagttt tgtccctgaa ggttgaaatg gcaccgatga taacgccatt
    10081 actgcgcacc ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa tctcttcgtc
    10141 gcggcgcggc gtaccgtcgc gtaacacttc cgagacatct accacctgtg accatgagtg
    10201 gctgagcgtc gacagtttct catcgtcctg cgacttacgg taattcagca attcttgtgc
    10261 ggcatcgttg atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc cttctttgat
    10321 agactgcaac atggcctggc gctgctcaaa cagcgtggag atttcgtagg gttccaggcc
    10381 gaaaaggatt tttttcagta ccttaaccag aatgcaggtg ccaatcagtc cgaccagcat
    10441 gccaaataat accgaccaga taatgctcca gcgactgtca ttgatttgtt gggtcacgcg
    10501 gcttaactca aggccgatcg ccaccacgcc aatttgttta tgattttcat cgtagatggg
    10561 ggtaaatacg cgtaaagcct gcgccagaaa accgcgattg atagcgacat tttctttgcc
    10621 attcagcgct ttaaggatgt catcaccttt aaatggctga ccaatacgct gggcttcagg
    10681 atgcgagtag cgaagacttt gcatatcggt aacgacaata aacagcagat cgttgcgttt
    10741 gcgcacggct tccgcgatgg cctggattcc gctctcctgc ggttttttct gcaagccctg
    10801 acggatttcc ggcgagtcgg cgagggtacg cgccactgcc agtgccttgt tggccagccc
    10861 gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga tgcaccacca atagcaccga
    10921 gaacagtacc gcgctgacca ttaagatcac tgtggtactc aatttcatcg gacgtttgcg
    10981 taacatacgg tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa tttcttaagc
    11041 attatctctg atgaggcggg taattcaaag ggagtaagaa tgattggcta tataggggaa
    11101 gagactctgg caacggaaac tgccagtgct gtatgaagat tccagggcta tgcttatagc
    11161 gataatcata ctgatgagag agggaaagtc atggatcagg cgctactgga cgagggttat
    11221 cgctgttata ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg tcagcattct
    11281 ggcaattgcg tacgtggcaa cggcaagtta tttaatctca aacgaaagcc gtggatcatg
    11341 ccggatgaag tcgacgtcgt cactgtggtt aaagtgattg atacgtgccc gagcggcgcg
    11401 ctgaaatacc gtcataaata agcgagggta aaatggaaat acgcgaaggc cacaataaat
    11461 tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg ccgaccggag
    11521 agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg caggggattg
    11581 gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc
    11641 cactatgccc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat gatattcgca
    11701 gttgatggga gagtacagag tcacgatatt tttcatgctc tccgcggagt gatgcaggag
    11761 agcatctgaa gggtaggggg atgcacaaag aatgggcaga gagcgcgttt ttttgtccca
    11821 agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc atcaatcggg
    11881 gttaataatt tttgcgtccc cagagcattc aatattgata ggagtcatat tatggaaggt
    11941 aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta ttcctcagtg
    12001 ttcttaaggt taagatcatt gatgtatgat atgaatttct cttctatcgt ggctgatgaa
    12061 tatgggatac cacgacaatt gaatgaaaat tccttcgcaa taacgacatc gttagccgca
    12121 agtgaaatcg aagatttaat caggcttaaa tgcttagact taccggatat tgattttgac
    12181 ctcaacatta tgacagttga tgactatttc cgtcagtttt acaagtagcc aggaaggata
    12241 agaagaacat aaaaaatggc actattctct aaaatattaa ttttttatgt gattggtgtg
    12301 aacatatcct ttgtcattat ctggtttatc tcacatgaga aaacacatat tcgtttactt
    12361 agtgcattcc tggtcggaat aacctggcca atgagtctgc ctgtggcatt acttttttct
    12421 ctcttttagg agctgtagtt gagtggaccc gctggttatc gtgaaacgaa gcgtctctcc
    12481 tcaaaactgg atttgcggaa cgacgaacga agcagtcgcc tgactgcttc gttcattaaa
    12541 gtgaaattta tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga atagtatgac
    12601 tgtttgtaaa cagcataatc attcggtcga taccaatacc cagaccagcg gttggcggca
    12661 gaccatattc cagcgcagtc acgtaatctt cgtcatagaa catggcttcg tcgtcacctg
    12721 cagctttagc gttaacctgt tcctggaaac gttcagcctg atcttctgcg tcgtttaatt
    12781 cgctaaaacc attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt
    12841 ctgggttaac atcattacgg cgtgccagcg gtgacacttc tgccggatat tccgtaataa
    12901 aggttggctg aatcagatgt gcttctgcca cttcatcaaa gatctccgtg acaatacgtc
    12961 ccagccccca gcttttctct accgtaatac cgatagattc agctaatgct ttagcagcat
    13021 caaaattatc caggtcggcc atatcggttt ctggacgata ttttttgatt gcttcgcgca
    13081 tggtgagttt ttcaaacggt ttgccgaaat caaacacatg ctcgccataa gtgactttag
    13141 tggtacccag aacctcttgt gccagggtgc ggaacaggga ctctgtcagt tcaatcaaat
    13201 cgtggtaatc cgcatacgcc atgtagagtt ccatcattgt gaactcagga ttatggcgaa
    13261 cagaaatacc ttcattacgg aagttacggt tgatttcgaa tacccgttca aaaccgccta
    13321 caaccagacg tttcagatac agctccggcg cgatacgcag gtacatatct aaatccagag
    13381 cgttatgatg ggtaataaac gggcgagcag atgccccacc tggaattacc tgcatcatcg
    13441 gggtttctac ttccataaag ccgcgcgcga ccatgaactg gcggatagcg gccagaattt
    13501 ttgaacggac aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc agataacgtt
    13561 gacgataacg gacttcctga tcctgcagac catggaattt atctggtaaa ggacgtagtg
    13621 cttttgtcag caggcgcaac tcagtacagt gaatggaaag ctcaccggtt tgcgtcttaa
    13681 acagcgtacc gcgggcaccg ataatgtcac ccagatccca ttttttaaac tgatcgttat
    13741 aaacaccttc tggcaggcta tctcttgcaa cgtacagttg aatacggcca ccgacatcct
    13801 gcaacgttac aaaggaggct ttccccatga tacgacgggt catcattcgg ccagcaaccg
    13861 agacttcaat gtttaaggat tccagttcct ggttatcctt cgcatcaaac tcttcgtgca
    13921 gctggtcaga ggtatggtcg cggcgaaaat cattgggaaa cgccacacct tgctgacgta
    13981 gtgccgccag tttttcgcgg cgatttctca gttcatcgtt aaaatcaata gcctcattgg
    14041 ctccccgtgt ttcttgttca gacattttgg ttcctctaaa tccagctttc aatttagcgt
    14101 agataaagag acagatcggt acactgtatt atctgcctcg actattaata actcagttta
    14161 tcttatcgaa actaacgctt acctcaagtt gatatcacta ataaataacc atcaaaatgg
    14221 tttaaatgac ttattaatct agatttgcta gtgttataaa ctgaaagtta atttattcat
    14281 aaccaattgt ttttacgaca gttaatcgtt gctctcctgt atcatattcg tcggcgtgct
    14341 aaacagaaaa cgggtagcaa aggccaatac gacgatgatc gcgacacaag ccaacgtcca
    14401 ctctcccatc tgagaaaaga atcgctggta agctgcaatt gccataccgc taatttgcga
    14461 ctctgtcgtc tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag
    14521 catataaata ccggttaata cgccagacat tttcagacgc gtgatttgcg caatcgccac
    14581 cgggtcaata aagagttcgg caaagcccat tagcgccagc ccggatatca tcacggccat
    14641 tgacgcttga ccgccagccg ctgcatgtcg ggcatcaaat gccaacaaca taaagccaca
    14701 agccatcagc agtaagccaa aggcaaactt cagccagacg cgcaatgttg agttgccgcg
    14761 gctttctggc gacgccagcc aggccagtac aaccccagcg agcatcactg caattgcatt
    14821 caccgactgg aatagtgctg taggtacttc aatattgaat gcctgacgat tcacaaagcg
    14881 atcgataaac aagctgatgg tactgccgcc ctgttgtgcc agtacccaga acaacgtccc
    14941 gacaaacatc aacaatacaa tttgccaaag agcacggcgg tgttcgggga atttgatcat
    15001 catgcgggca atgatttgtg cggcaatgag gcaaacgatc gccagcaaat atccggacca
    15061 gtcgttctcc agcagcaggg taaaaaatac tggggctaaa cagagcatca ccactaacca
    15121 gctccatact ggtaaggcaa atttcacgct agtgagcgct tttttatcca tactacgcgt
    15181 ggactggaaa tgacgatgcc cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc
    15241 cgcaagggca aagccaacat gccatccata ccactgagca gccaggccgc aggcgattgg
    15301 ggcggcgata gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg
    15361 atgatcgttc tcgtcgtaga gctcgccaag caaacagctg atgtttgatt tgaataaacc
    15421 gtagccacaa ataatgattg ccagcgccag atacaggcta aaggttgaat ttgtatcaat
    15481 gcccagcacc acatggccaa gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt
    15541 gccgagcagg cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa ccagagaagc
    15601 atatgcgctg aacaggctga tggcatggtt atcatcaaaa ccaagctgat gggtgagata
    15661 gagaatgagt aaggcacgca tgccgtaaaa actgaagtac tcccagattt ggatcgccac
    15721 gatatagtat atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa
    15781 aagggaagtg gcaagccact tcccttgtac gagctaatta ttttttgctt tcttctttca
    15841 ataccttaac ggtatagcgg ccatcagcct gacggtatgc accgtgaata tcggtttcaa
    15901 agcccggata gtgagcgccg atttcacaca gcatctgcag gaactccaga accggacggc
    15961 tttcttcggt gatcatttca cccggcatta ccagaggaac tcccggcgga tacggaagga
    16021 tcatattggc gttaatacga cctaccattt cgtcgaggta aacttcttcg gtcataccgt
    16081 gcagctcttt ctggaatgca gcatacggag tcattaccat cgtcggcagc acttcaaatg
    16141 cgcggtacat cagatccggc agattgtggt gaacaatcag tttgtggata ttctgagcca
    16201 gttcctgaat acgcatgttt tcatagaatt caggatcttc acgatacaga gacggcagca
    16261 tgtttttcac acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga gcacgcagca
    16321 ggctcagtgc tttggtctta tcgataccga tgctgaacag gaacagcagg ttatacggac
    16381 cggttttctc aacaacgatg ccatgttcgt cgaggtattt cgccacgatg ctggccggaa
    16441 taccaaagtc gctcatggtg ccgtcttttt ccatccccgg agtcagcagg gtgactttga
    16501 tcgggtcaag atacatgtgc tcgttatcga tgtttttgaa gccgtgccag gtgctgtcag
    16561 aacgcagcgg ccagcattca gtcgtatcga tatgatccgg ctgccataca tcaaagaacc
    16621 agccatcaga ttccgttctc agacgtttga gctctttacg gaatttgatc gcacgttcaa
    16681 tggaaccgtt gatcagacgt ttacctgcat tgcctttcat catcgccgca gcggtttcag
    16741 tggacgccac gataccgtag tgcggagaag tggtggtgtg catcatgtag gcttcgttaa
    16801 aggtttcttc gtttacgtca cctttaacgt ggatcatgga agcctgagag aacgccgcca
    16861 gcagtttgtg agtggactgg gtttcgtaaa tcactttccc ttctacacgg ccaccgctca
    16921 taccgcattt accttcgtaa atcggtgaga agttggtgta aggcacccac gcggagtcaa
    16981 agtggatgga tttcacatcc agtgttttct tgatgaagtc ggtgttgtac agcagaccat
    17041 cataggtaga gttggtaatt acagcatgta ccggccaggt tgcgtttggt gtttctttca
    17101 cgcgcttagc aatggtagcg tgctggaatt cactctgtgg gataccacca agaataccgt
    17161 aagcgttacg ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc atcaggtggg
    17221 tcagcgattt gtggcagtta cggtcaatca gaatggtgct gcctgccgga gcagagtaca
    17281 tgccaacaat tttgttcgca gtggaagtac cgttggtcac catgtagctg cggtctgcgt
    17341 taaagacgcg agcgatatac tgttctgctt ctttgtgtgg accactgtga tccagcagag
    17401 aacccagttc agatactgaa atggaaatat cagatttcat ggtattcgga ccaaagaaat
    17461 catagaacag gctacctacc gggcttttct ggaatgcagt accgcccatg tgaccaggag
    17521 tacagaaagt atatttacct tcacgaacat atttaaacag tgctttagtc agcggaggca
    17581 gaatagtgtt gatatattcg tcagtggtct gcttgatctt attagcaata tcttcagcag
    17641 cacccagcgc atattcaaag aagctaatct gtaaacgcag gtcattcagg cttacatcga
    17701 gagtggaata cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt
    17761 cttcgcacag ctcgagatta tatttatccc agtcaaaaat aacgccgcac agacgcgcat
    17821 tgttttcgat cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca
    17881 gacgttcaag cgcgcgatga agttcacgga tgggttcttc tttaaaataa acccccatgt
    17941 gattcaatat tgcaataacg ttcatagtca tatctccagg taaaaaaggc ccctcccaac
    18001 acatgggaca aaatgaaagg aggagcctcg gaaaatactt ttaattaatg tgcgttagac
    18061 gcggtgtggt tatccattga gtggctctgg cgctcgtgca ttttgcgagc gtagaacatc
    18121 aggataatca ggctgacgat gaaggtacct gccagctcga aggagcttgc gcccatcagc
    18181 gcgatgaagc agaacacaca acccagtaca gagcagatca ggctgacaaa gttgcggatg
    18241 ttaacgcctt caaaacgaat caggtcaacg caagagtaga agtacggcag catagtcagc
    18301 agtactgcga taccggtcag ttcaccgaac aggtcagatg ctttaccacc ggcagagttc
    18361 atcagagtga tgaggatcat cagggcagtc attttcactg cagccagcag cagacctttt
    18421 ttcggaatac cgttgctgtc gacttcacca taaactttcg ggaagttacc gtcgttagcg
    18481 gcacgtacac ctgcctggcc taccaacatc atccaggagc ccagagaagt caggcacgca
    18541 aaggcggtga atgcagaaac cagcggcgca gcccagttac cgaggatggt tgaagcactg
    18601 attgcaaacg gagcaccgga agctgccatt acagaagatg gatacatacc ggaaagcacc
    18661 tgagtcgcag cgatgtaaac aatacctgct aaaccagtac ccagcatggt tgccagcgga
    18721 acggtacgtt tcgggttttt aaccatacca gtacttactg ctgcggattc aacacccacg
    18781 aaggcccaca ggcagagcag aatactttta atgatcgcat gaccatcagt ggtatccgca
    18841 gtattccagt tagctgcata agttgccgca tcaaaccaat gccagccaac aatagcggtc
    18901 attactaccg gaataagaac cagcaccaga ccaatagtgg ttaaacggct tacccaagta
    18961 ccgccgagca tatttacaaa ggtaaatacc cagacgatag caatacaggc gatacccgcc
    19021 ggaacaggat catttaatac tgggaagaag gtggaaagat aagataccgc ggtaatacca
    19081 atcgccaggt taccaatcca gttagcatgg taataaagaa cacctgtctg aaaaccaaat
    19141 gcaggggaaa tttctccggc ataagcaatt gggccacctt gttgcgggtt ttttgttgcc
    19201 agtcgggcat atacatacgc cagcgacatt gcaccaataa tagagataat ccaaccccag
    19261 atagcaatac caccgatact tgctaggttc gcaggtaata atgcaatacc gctccccatc
    19321 atattaccgg caacaacacc ggtacaggca aatagcccga tcttcttggc agaactcatg
    19381 ctcttctcct aatttcattt ttgaatttgg agtccgggtc atgatgtata actatttcct
    19441 gaccagacca aactggcgct aagaatactc acgaaaaaag gattaatcct aaagattagg
    19501 tgaaataaca caaaagtttc tgtaagtgag aacttgaggt tttttattaa cacatcagga
    19561 tcgcaagttg atatcatgaa aagataaaca ttcaatgttt acaatggatt gcg
//
LOCUS       DAZFKM010000093        19452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFKM010000093 DAZFKM010000000
VERSION     DAZFKM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..2339
                     /gene="katP"
                     /locus_tag="ACHKAY_003381"
     CDS             129..2339
                     /gene="katP"
                     /locus_tag="ACHKAY_003381"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFO5702004.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            2383..2772
                     /locus_tag="ACHKAY_003382"
     CDS             2383..2772
                     /locus_tag="ACHKAY_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5702005.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            2886..3653
                     /locus_tag="ACHKAY_003383"
                     /pseudo
     CDS             2886..3653
                     /locus_tag="ACHKAY_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    3166..3276
                     /locus_tag="ACHKAY_003383"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(3733..4128)
                     /locus_tag="ACHKAY_003384"
     CDS             complement(3733..4128)
                     /locus_tag="ACHKAY_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5702006.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(4128..5087)
                     /locus_tag="ACHKAY_003385"
     CDS             complement(4128..5087)
                     /locus_tag="ACHKAY_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO5702007.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5360..6262
                     /locus_tag="ACHKAY_003386"
     CDS             5360..6262
                     /locus_tag="ACHKAY_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO5702008.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTYLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWYQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSHP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            6259..6570
                     /locus_tag="ACHKAY_003387"
     CDS             6259..6570
                     /locus_tag="ACHKAY_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702009.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            6646..7329
                     /locus_tag="ACHKAY_003388"
     CDS             6646..7329
                     /locus_tag="ACHKAY_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO5702010.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAVWIRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNSIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNRFEPEAA"
     gene            7329..7547
                     /locus_tag="ACHKAY_003389"
     CDS             7329..7547
                     /locus_tag="ACHKAY_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702011.1"
                     /translation="MNYSGFEDMREQVAELANTMCDLRTTMNEMEQRYSDADTLPERL
                     VRQTLFRASHLFMKAYNEILELDYCFKD"
     gene            7507..7993
                     /locus_tag="ACHKAY_003390"
                     /pseudo
     CDS             7507..7993
                     /locus_tag="ACHKAY_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8038..8277
                     /locus_tag="ACHKAY_003391"
                     /pseudo
     CDS             8038..8277
                     /locus_tag="ACHKAY_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKAY_003392"
                     /pseudo
     CDS             8272..8520
                     /gene="psiB"
                     /locus_tag="ACHKAY_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
     gene            8517..9236
                     /locus_tag="ACHKAY_003393"
     CDS             8517..9236
                     /locus_tag="ACHKAY_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO5702012.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            9233..9830
                     /locus_tag="ACHKAY_003394"
                     /pseudo
     CDS             9233..9830
                     /locus_tag="ACHKAY_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10292..10792
                     /locus_tag="ACHKAY_003395"
     CDS             10292..10792
                     /locus_tag="ACHKAY_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO5702013.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPVNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(10794..11414)
                     /locus_tag="ACHKAY_003396"
     CDS             complement(10794..11414)
                     /locus_tag="ACHKAY_003396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702014.1"
                     /translation="MDLSDGAATATARGSCPGADAKVFDACGKKFCGWYIFPWTAAPC
                     CGRFSPRKVHSGKQAAGVAAEGLTAPSGAVALHTVRCAGSAVSGATGAARPERSLQAW
                     FFRHRTVAAARNRASGFGGDWRVRQQLPVGVGPTTGCRQAAGSRRVSPPPGIRTTETT
                     KQKNASHHDGQEGQRRRRGPPVFQAGVSLPGFSGGPAGHTPAGAGE"
     gene            11520..11954
                     /locus_tag="ACHKAY_003397"
     CDS             11520..11954
                     /locus_tag="ACHKAY_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO5702015.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKSAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            12047..12313
                     /locus_tag="ACHKAY_003398"
     CDS             12047..12313
                     /locus_tag="ACHKAY_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702016.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERACH"
     gene            12378..13268
                     /locus_tag="ACHKAY_003399"
     CDS             12378..13268
                     /locus_tag="ACHKAY_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO5702017.1"
                     /translation="MSKKKNTTTPTPHDAAFWSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSEAELKNITH"
     gene            13268..13513
                     /locus_tag="ACHKAY_003400"
     CDS             13268..13513
                     /locus_tag="ACHKAY_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702018.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKKGALEV
                     AREAACRLRDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(13544..13795)
                     /locus_tag="ACHKAY_003401"
     CDS             complement(13544..13795)
                     /locus_tag="ACHKAY_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5702019.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(13829..14024)
                     /locus_tag="ACHKAY_003402"
                     /pseudo
     CDS             complement(13829..14024)
                     /locus_tag="ACHKAY_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14374..15222
                     /locus_tag="ACHKAY_003403"
     CDS             14374..15222
                     /locus_tag="ACHKAY_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5702020.1"
                     /translation="MNQTLPTADLNTAGTTDIIPSMAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTYTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQELIDIWRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            complement(15308..15643)
                     /locus_tag="ACHKAY_003404"
     CDS             complement(15308..15643)
                     /locus_tag="ACHKAY_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO5702021.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKAY_003405"
     CDS             15875..16207
                     /gene="mobA"
                     /locus_tag="ACHKAY_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5702022.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            16219..18939
                     /locus_tag="ACHKAY_003406"
     CDS             16219..18939
                     /locus_tag="ACHKAY_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO5702023.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLATAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWEPPSPGM"
     gene            complement(19160..>19452)
                     /locus_tag="ACHKAY_003407"
     CDS             complement(19160..>19452)
                     /locus_tag="ACHKAY_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HFO5702024.1"
                     /translation="QYDTDIESRANELWKKAVHTINNTTREERRVCYITLNRPEEEHS
                     DPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY"
BASE COUNT         4645 a         4658 c         5334 g         4815 t
ORIGIN      
        1 tgcggcttgg aaccccttag tattttttgt ctgtagtatc tatcccagca ataggtatat
       61 cctgttgcat caataaagtt gacttttgta tacaacatgc gaatttccct taatccggag
      121 ctattcgtat gataaaaaaa actcttcctg ttctgattct tctggcgcta tcggggagct
      181 tttctaccgc tgtagccgct gataaaaaag agactcaaaa tttctactat ccagaaacac
      241 tggatttaac tcctctgaga ttacacagcc ctgaatcaaa tccctggggg gctgattttg
      301 attatgccac cagatttcaa cagctggata tggaggctct gaaaaaagat atcaaagatt
      361 tgctgacaac ttcccaggat tggtggcctg cggattatgg tcattatggt cctttcttta
      421 ttcgtatggc ttggcacggt gccggaacat acaggacata tgatggccgg ggaggcgcca
      481 gtggtggtca gcaacgtttt gaaccgctga acagctggcc ggataacgtt aatctggata
      541 aagcccgtcg attgctgtgg ccagtcaaga aaaaatacgg ctccagtatt tcctggggag
      601 acctgatggt cctgactggt aatgttgccc ttgaatccat gggatttaaa acgctgggat
      661 ttgctggcgg aagagaagat gactgggagt cggacctggt atactggggg cctgacaaca
      721 agcctcttgc agataaccgg gataaaaacg ggaaacttca gaaacctctt gccgccacgc
      781 agatgggact tatttatgtc aatcctgaag gccccggtgg aaaaccagat cctctggctt
      841 ccgcgaaaga tatcagggaa gctttttcac gtatggccat ggatgatgag gagactgtgg
      901 ccctgatcgc gggagggcat acatttggta aagcacatgg tgcagcgtct cctgaaaaat
      961 gtattggcgc agggcctgat ggtgcacctg tggaggagca gggactggga tggaaaaata
     1021 aatgtggtac aggaaacggc aaatatacca tcaccagtgg cctggaagga gcctggtcga
     1081 catcgccaac ccagttcaca atgcagtatc tgaagaattt atataaatat gaatgggagc
     1141 tgcacaagag tcctgccggt gcttatcagt ggaagcctaa aaaagcggca aatatagttc
     1201 aggacgcgca tgatccgtct gtcctgcatc cgttgatgat gtttacgacg gatattgctc
     1261 ttaaagttga tcctgaatat aagaaaataa ccacccgttt cctgaatgat ccaaaagctt
     1321 ttgagcaggc attcgcaaga gcatggttta aactgaccca ccgggatatg ggaccggcag
     1381 cccgatatct tggtaatgaa gttcctgcag aatcatttat ctggcaggat cctcttcctg
     1441 cggcggatta tacaatgatt gatggtaaag acattaagtc gctgaaagag caggttatgg
     1501 atttgggtat ccctgcatct gagctgataa agacagcctg ggcttcagct tccacatttc
     1561 gtgtgactga ttatcgtggg ggaaataatg gtgcccgcat caggttacag cccgaaatta
     1621 actgggaagt taatgagcct gaaaaactga agaaagtact ggcatccctg acctcattac
     1681 agcgtgaatt taacaaaaaa cagtctgacg gaaagaaagt gtcgttggct gatttaattg
     1741 ttctttcggg taatgctgca atcgaagatg cggccagaaa agccggggtg gaacttgaga
     1801 ttccctttac tccgggaaga actgacgcct ctcaggagca gacggatgtt gcctcattca
     1861 gtgtactgga gccgacagca gatggattca gaaattatta ctcaaaaagc agaagtcata
     1921 tatcgccggt tgaaagcctc attgataaag ccagtcagct ggatctcacc gttcctgaaa
     1981 tgacggcatt actgggtggt ctgcgggtaa tggatattaa tacaaataat tcttcgttgg
     2041 gagtgtttac cgatacccct ggtgttctgg ataacaagtt ttttgttaat ctgctggata
     2101 tgtcaacacg atggagtaaa gcagataaag aagatacata caatggattc gatcgtaaaa
     2161 cgggagcatt aaaatggaaa gcatcctctg ttgatttaat cttcagttca aatcctgaat
     2221 tacgtgcggt ggcagaagta tatgcctcgg atgatgcgag aaataagttt attcatgatt
     2281 ttgttaaatc gtggaataaa gttatgaata gcgatcggtt tgatttaaac aataaatgat
     2341 aaaactgatg agggagggca cctccctcat gaaggagaaa ttatgcagaa aagcaataaa
     2401 gttataatca gctgtggtat ggcattatat tttttgtgtg gggggattac agtggcagaa
     2461 gaacttgatg aaagcatgca agttcttgca aggaatatga gtgtacttca gtcatcaatg
     2521 gataaaaagg agcttcttga agctcttgac agaatggatg atgctgtaga tgagtcaatg
     2581 aagcgtcttc ctgagagcat ttcaccggct gatgaagaag ggaagaatga ctatataaaa
     2641 gaacttcaaa agcttgaaaa agagattatg ctggcgaaac aaaaagtctt atcgggacag
     2701 ttatcagata tacccgattc ggtaaacaag atgaataaaa tcagagttga ggggcatgat
     2761 aaattcagat aaatcagttg gttgattcct gcgggacgaa tacatacgtc aggaacataa
     2821 ctttatggca gtgaaccgcc ccgggaatcc tggagactaa acttcctgag aaagaggtaa
     2881 acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggcg attcatatgg
     2941 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttgttcc attgcccaaa
     3001 gattggctgt acaccggaga ctctgcgtgt ctggggacgc cagcatgagc gggataccgg
     3061 gagcggtgat ggtgggctca ccagcgctga acgtcagcgt ctgaaagagc tggaacgtga
     3121 aaaccgtgaa ctgcgccgca gtaacgatat ccttcgccag gcttccgctt attttgcgaa
     3181 ggccgagttc gaccgcctct ggaaaaaata atgccactgc tgctgcgtga gcagtacggg
     3241 gtcggaccgg tatgcagtga actgcatatt gccccgtcaa cgtattacca ctgtcagcaa
     3301 cagcggtatc atccttataa acgtagtgcc tgtgcacagc gcgatgactg gctgaagaaa
     3361 gagatactgc gcttatacga tgagaatcat caggtatacg gtggcgtaaa gtctgacgtc
     3421 agttgttaca ggaaggtatc agggtggcca gatgtatagt ggcgcgcctc atggcggtta
     3481 tgggacttgc cggtgttctc cggggtaaaa aggtccgcac taccgtcagc cggaaagctg
     3541 ttgccgcagg cgacagcgta aaccgccagt tcgtggcaga acgtcctgac cagctgtggg
     3601 tggcggattt tacttacgtc agcacatggc agggcttcgt ctgtatggcg ttcgatttag
     3661 ggcactaaaa ccgctgctgg tccctcataa taaaagaaga gcaggtaata gacttaacct
     3721 gctcttcttt tattattttt ttaacataga aagattgcgt ctggtttgtt cttcgaaatc
     3781 atcttttgaa gtgatgctct ctgatttgtt ttctgaagat gcccctatga tgttttttaa
     3841 cacacttata tcaattgatg tagatgtgta tcctgttgtt tgctctataa gtttcaccag
     3901 attttctgca gtgattttct cactgaaaaa accactgatt agcgttaaca gtcgggggtc
     3961 aacagaataa agagctgctc cgcaaataag agattgtctg tagaagtctc cccgcatttg
     4021 tatcgggaga gagtctattg tttgttgtgt caggttatcc tgcggataaa gtgtcgggtg
     4081 caggtatgcc acaactttct ttcttttttt cggatcactg ctttccatta cgcatgcacc
     4141 attgctttca ccaatgcaaa ctgaggttca tccaccttgt tcactttcag attcgggaaa
     4201 tattgcttta tgtgagggta aataagctcg gctcctccgc cagtgagata gattcggttc
     4261 attcctgaga atgttgaaat ttcctgcctg atgctttctg ccagagattt cacttccctg
     4321 tctattacat gagtaatgtt ttttatttca gctacatcat ttattaatgt ctggagatat
     4381 tcattatcat gtctgttttt tattaactca tcagcaataa aataattaca tggtgtggag
     4441 gctttcgtca gcgcattcat gacggtatga gtgatgcggg atgtcccgat ttctgaatac
     4501 ccacggattt cagaaatgcc ttcaaatgca ccgagaatta aaccacaatc cagagtggtt
     4561 cctccaagat cgataatcag gctgcgttca agtttgttta ctttatcttt ttttagtgat
     4621 tcaaatgcag ctggcaaaga ttcaggcatt acattaactt ttttaatttt aaatgtttcc
     4681 cctttattca gagatatctc acggagaaca tttgcctttt ttctttctat tctttcttca
     4741 tttggttgat tgtcattatc aaagaattcg gttacaggta atgtcactgt cagttctaca
     4801 tcctgtggct gaaggccgga tgtcagcagt gcatgatgta tggcaagcaa attggtggtg
     4861 ctgtattgat aactgacatg tgttgtacct atgacagcag tactgcccaa atcataggtg
     4921 tatttttttc catccacgac gtaattgtaa actttgccgg cattaaacag tcccgggttc
     4981 cagccttctt taaagctgtt tcctgacaga ctggttctta cctcgccatc ttcaagccag
     5041 gccagtttta cgttagttga accatcatcg catgatactt ttagcatttt ttcttgcctc
     5101 gttttgagtt tttatttatc tcaaattgag catcctttat ctagatgcat ctttttgagt
     5161 tttttgttgt tccttattgt gtctcatttt gggttcatgt caagcgatga ggctctttta
     5221 gagtctcttt gcattcttgt tgtgactcag tttgagtctc tatcgatttc tcattttgcg
     5281 tttcgaactg agacggaaac tacttaacca ggccgcatgt gagaggagaa ttcccatgcc
     5341 aaactggtgc tcaaatcgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa
     5401 acgactggcc agcggtgccg tcacaccgct ttatcgccgc gccacaaatg aaggtattca
     5461 gctgtttctg gccggaagtg ccggacttct gcagaccact gaagatgtgc ggtttgaacc
     5521 atgccccgga ctgacggctg ccgggcgtgg cgttgtatcg ccggagaata tcgcgttcac
     5581 ccgctggctg acatacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct
     5641 gcatgaactc tggctgcaga gcggtactgg acagcgtcgc tgggaaggat taccggacga
     5701 tgtcagggat accatcaccg cacttttcac cgcaaaaaga ggtgactggt gtggcttctg
     5761 gagtaacgag gatgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac tgccggaaaa
     5821 aaccatgccg tttgacctgc tgacggttct gccgacccgc ctggatgttg aagtgaatgg
     5881 ctttaacggt ggtgttctga acggtgttcc ttctgcatat cactggtata cggaacagta
     5941 tggcgtgaag tggcctgtgg ggtatgaggt gaatatcagt agtcagggag acaacttcat
     6001 tcaggttgat ttcgacacgc cgtggtatca gccggaaagc gacgttattg cagaattaag
     6061 ccgccgtttc agctgcacac tggagcactg gtatgccgaa cagggctgca atttctgtgg
     6121 ctggcagcga tatgagcgtg gcgagcttgt tgatgtgctg tggggggagc tggaatggtc
     6181 acatccgaca gatgatgacg agttgccgga agtcaccgga cctgcgtgga tagtcgacaa
     6241 ggtggcgcat tatggcggat gaagtatgac ggagacgggc gggcaactgc ccgtttcttt
     6301 tccggtaaag gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcat
     6361 aaacgctgtc tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc
     6421 tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct
     6481 tcaagccgct ttcgcggcat agccttgccg tcaaaaagac gaaggctgcg gtattccacg
     6541 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggt aacgggcacc
     6601 acaggggtgc cctcccggtg ccctctgtaa gagaaggagt tttgtatgtc ccgttttgtc
     6661 ctcggtaact gcatcgatgt tatggcccgt attcctgata acgccattga tttcatactg
     6721 actgacccgc cgtacctcgt cggtttccgt gaccgttccg ggcgcaccat tgccggtgac
     6781 gttaatgatg actggctgca acctgccagc aatgagatgt accgcgtact caaaaaagac
     6841 gcgctgatgg tcagcttcta cggctggcac cgtattgacc gctttatggc ggtctggata
     6901 cgcgccggat tcagcgtggc agggcacctc gtgttcacca aaaactacac atccaaatct
     6961 gcgtatgtcg cgtaccgcca cgaatgcgct tacatcctgg caaagggcag tccggcccgg
     7021 ccccgtaacc cgctgccgga tgtgctgggc tggaaatatt cgggcaaccg ccatcacccg
     7081 acggaaaagc ccgttaccag cctgcaaccg ctgattgaga gcttcacaca cccgaactca
     7141 attgtgctgg acccgtttgc aggcagcggt tcaacctgcg tggccgccct tcagtccgga
     7201 cgccggtata ttggtatcga gctgcttgaa cagtatcacc gtgccggaca gcaacgcctt
     7261 gccgccgtgc aacgggccat gcagcagggg gccgcaaatg ataacaggtt tgaaccggag
     7321 gcagcatgat gaattacagc ggatttgaag atatgcgtga acaggtggct gaactggcca
     7381 acaccatgtg cgacctgcgc acaaccatga atgagatgga gcagcggtac agcgatgcag
     7441 acaccctgcc ggaacgtctg gtgcgtcaga cgctctttcg tgccagccac ctgtttatga
     7501 aggcatataa cgaaatactg gaactggatt actgcttcaa agattgaggg gaaagagtat
     7561 gtacggaaca tgcgaaacgc tctgccgggc gctggcagca aagtatccgg gagacacgct
     7621 gttgatgctg gttatctggt ccccggaaga gattcaggcc ctcgctgacg gaatggatat
     7681 ttccctggcc gatcatgaaa tcaggaccat cctggcgcgc ctggaggaca tcccggaaga
     7741 ccagcggact gagtccggta tttcttcctc cgcggcgatg gagattatca gcaacgtgag
     7801 cgaaaaccgc caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga
     7861 acaggcattg tggaagcgtg aatgggccgc ccgtgattac ggcctcgccg tcccggaatg
     7921 cgttacccgc cgtcaggcgg tggttaatca ggcccgcatc ctgctgaaaa acaacacgca
     7981 cgaaaacgac tgatgttatc gccgcctccg ggcggcgatt cagggagacc atgaatcatg
     8041 aataaaacgc tgaatgcact gatttgtcgt catgcccgcg acctgctgct ggcgcagggc
     8101 tggccggaag agacggatgt tgaccagcgt aacctgaagt atccgggctg gatcagcatt
     8161 tacgtgcggc tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgta
     8221 ctgccgccgc acttggcctc cgccattcag aaactgaccg gaaccggggc ggaacttggt
     8281 ttcacgccag cccacgaacg tttccacctg gcattatgtt cgccgggaga cgtctctcag
     8341 ctctggatgc tggttctggt gaattgtggt ggacagcctt tcgccgtcgt tcaggtgcaa
     8401 catatcttca cgcctgtcgc tatcagtcac acgctggcgc ttgccgcgac acttgatgcg
     8461 cagggataca gtgttaacga catcatccat atcctgatgg cagaaggagg tcaggcatga
     8521 gcgcacgttc acgggcactg attcccctca gcgcagaaca acaggccgcc atgcaggctg
     8581 tggctgtcac agaacaacgt cgtcgtcagg gacgcacact ttcagcatgg ccttatgcca
     8641 gcgctttctt tcgctgcctg aatggcagtc gccggatttc gctgaccgat ctccgctttt
     8701 ttgcccctgc gctgacgaag gaggaatttc atggcaaccg tctcctgtgg ctggctgccg
     8761 tggataaact gattgaaagt tttggtgaag tctgtgttct tcccctgcca tccgatgcgg
     8821 ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga gcgtcgtcgt cagaaaacca
     8881 cgctgacaga gcagaaatac agccgccagc gggaacgtga ggcagaacga cgggaactgg
     8941 aataccagac atgttttgct caggcgcaga ttgaccttgc gtttcatact cccgccacgg
     9001 tcggaagctg gttgtcccgc tggtctggtg ttgttgagga gcatgatctg gaaacgattt
     9061 tctgggggtg gtgcgggcgt tttccatcac tgtcatcatt tgaccggttt ttctggcagg
     9121 aggaaccact ctggcggctg atttttgaag ccggtgaggc cggtcgtggt gcaccggtac
     9181 aggtacgtgc acttgagcag tggatgatcc cgaacaagct ggagaacgca atatgatgaa
     9241 atcagacgaa caatatcagg ttcccgtgtg gatgcgacct ctgttgccgt tgctctgcaa
     9301 taccggtggt aatgatccgg aggaacttct gaatgataca gaaaccaccg ccagtgcgaa
     9361 tattgtccgt tatgtactga tagttgcagt gcggtcgcag attggatctg ctgcagctac
     9421 tgtacaggaa aggactgttg cgcacagaga taccaggtgg cttttcaccg gaagaagcgc
     9481 aggaactcct ggataatctg gtgcgcagcc atatcagcaa ggcgctgtcg ggtgagcgaa
     9541 tggcagcccg tgacagatat gccgagctgg cctggattcg ccagcaactg gtggatgccg
     9601 cctggtttgt ccgtgccaca ctggaagcgc atggtacgag cgtcggaaat gagagtccct
     9661 ctgcacttcc agagacaatg ccggacatac agacacggga actggtgatg ctgatcaaac
     9721 gactggcatc ttctctgaaa gcggtgaaac cggacagtag cgtggtgcgt gcggcgcagg
     9781 actggctgtg cgacagaaaa cttgtggata tcacagatat tctccggtga accaggcatg
     9841 tatatttttc gcagggacac gccgatcatg atcgtttcat atcaggggtg gtctggaggt
     9901 catgcggcgt gtcctctgca ctcgccggaa taaggaagtc gccggcggat ccgcttttac
     9961 ccggccatgc ggggcatggc cttgtgggtt ttcagttatg tggcctcagc gtcgtgtgcg
    10021 ggctgtgccg tgcctccatc gtagccgggc ttctgcgggc gggatgcaag gtaagatgaa
    10081 ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc gttcacccgc
    10141 tcagatttcg cggtctcacc ctccaactcc cgtcagccgt gcgcgtcggg ctgcggcttc
    10201 ccttgcgccc ttgcatccct gcaggcccgg caaagatgga ggcacggcac cgcccggtgt
    10261 cgcagaacat gtgactatgg aggattcggg aatgtctgtt gttgcacctg ctgtatacgt
    10321 tggaacctgg cataaataca actgtggaag catcgctggc cgctggtttg acctgaccac
    10381 gtttgatgat gaacgcgact ttttcgccgc ctgccgtgct cttcaccagg atgaagccga
    10441 tcctgaactg atgtttcagg attatgaggg gttcccggtg aatatggcct ccgaatgcca
    10501 tatcaactgg gcctgggttg aaggcttccg ccaggcacgg gatgaaggct gcgaagaggc
    10561 ttatcgtctc tgggtggatg ataccggtga gacggatttt gacaccttcc gcgatgcctg
    10621 gtggggcgag gctgacagtg aggaggcttt tgcggttgag ttcgtcagtg ataccggcct
    10681 gctggctgac gtgccggaga cggtggcgct ctattttgac tatgaggcgt atgcgcggga
    10741 tttattcctg gactccttca cctttattga cggtcatgtg ttccgtcggt gatttactcc
    10801 cccgctccgg cgggggtatg gcctgccggg ccgccggaaa acccaggcag gcttacgcct
    10861 gcctgaaata caggagggcc gcgcctgcgg cgctgccctt cctgcccgtc atggtgtgag
    10921 gcatttttct gttttgtcgt ttctgttgtt ctgatgcccg gaggcgggga cacccttcgg
    10981 ctcccggctg cctggcggca tccggtcgtc ggcccgactc cgacaggcag ctgctggcgc
    11041 acccgccagt ctccgccgaa cccgctggcg cggtttcggg ctgccgctac ggtccgatgt
    11101 ctgaaaaacc aggcctgcag cgaccgttcc ggtcgcgccg cccctgtcgc gccggaaacg
    11161 gcgctccctg cacacctgac ggtgtgtaag gccactgcgc ctgatggcgc agtcaaccct
    11221 tctgccgcca cccctgcggc ctgtttcccg ctgtggactt tccgggggct gaagcggcca
    11281 caacacgggg cagccgtcca ggggaagatg taccagccac aaaacttttt gccgcaggcg
    11341 tcaaaaactt ttgcgtctgc ccccggacaa ctgccccgtg ccgtggcagt cgctgcccca
    11401 tcggaaaggt ccactgcggg gagaaacccc gaagaagtgg acggcagaag gggctttcgc
    11461 ccctccgggg gagcaaccgg tcggcccggc gacctgattc agcagaagga gacgttttca
    11521 tgacagtcag cagcacgatt tccgtttttt gccgcgacgg ggtgttccgt accgtttact
    11581 gccacctgca cggggagccg acctggaacg gtcgcatcct gcatacccac tatgccaccg
    11641 gtcagctggc cgaagccctg gttgaacacg gagatatccg ttgcctcggt ccccgttgcg
    11701 acaaatccgc cgggcatacg cttcagaacc cggtggacgg tgtgacggct tattacggac
    11761 gtgacagtgg tttccggatg gacagtgagg cgcgtgagta ccgttctttc atggaggcta
    11821 ttgccactga aagcactgaa gaggtgcgct tccattatgt gttcatcgac ggctactgga
    11881 aggtgatgta ccgcacgccg gaaggctgga agatgaaagc gctcgcgctg gcactgcgcc
    11941 gctgtccgaa atgaaaaaag gcagggcgat aaaccctgcc tttctccgcc ggcgcttccc
    12001 cgtacggaag tccggcatct caatcactat ctatggagat tatgccatga gaccatccat
    12061 tatcttcgca accgccgagt atgtaaagcg tctgcgtgaa gagtgcctgc gggagaataa
    12121 acccctgcac cgccataccc gcgtcagacg tcaggagctg gcacaggatg agattaaccc
    12181 ggacgtcctg gcgatgagcg gtcatatcgc cagacgctgc agtgagcaga agcgggtacg
    12241 tatccccgcc atgaaagtca gcgaatgggg ccacctgctc cgcgcgcttg aaattgagcg
    12301 ggcctgccac tgattaaccc ttgccgtctg cctgtgcagg cggcaggata cgttcagaca
    12361 tctcaccagg aggaaccgtg agtaagaaga aaaacaccac aacgcccacg ccgcatgatg
    12421 ccgcgttctg gtcgttcctg gcgaatcccg acgtcgccag agattttctg gaactgcatc
    12481 ttccggcgga gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa cccgccacct
    12541 ttgttgagcc ggacctgcat cagtacgcca gcgatatcct ctggagtgtg aaaaccaccg
    12601 ggggtgaaga cggctatgtt tatacgctca tcgagcacca gagcaccgaa aatctgtaca
    12661 tgcctttccg catgttacgt tacagtgtgg cggcgatgca gagacatctg gagcagcaca
    12721 aaacattgcc actggtgatt ccggtgctgt tctatcatgg tgagcgcagc ccgtacccgt
    12781 acagcatgaa ctggctggac tgttttgagg aaccggcgct tgcggctaaa atatacacaa
    12841 agccgtttcc gctggttgat atcactgtcg ttgatgacaa tgaaatcatg aaccatcgcc
    12901 ggatggccgc actgacgctg ctgatgaagc atatccgcca tcgtgacatg atggagctgc
    12961 tggacaaact cccgcaggtc atggtggaaa tttcagacga gcaggtgcgt gttctgattc
    13021 attacatcgt taacgcaggg gactctgtat caccggaatt tatgcgggcg ctggctgagc
    13081 gtctgccgca gcatgaggat aaactgatga ctatcgctga acgtcttgag caaaaaggtc
    13141 gccaggaagg agcgcttgaa aaagccctgg ctattgcgtg ccagcttcag aaaatgggga
    13201 tgacgccgga gcagattaag caggctaccg gactttccga agctgaactg aagaacatca
    13261 ctcactgatg agaaccacaa agcccggcaa aatgccgggt ttttttgtca gcaggagcag
    13321 agcaaacagc atgacgattg cagaacgtct tatacagaaa ggtttcgacg aaggttttaa
    13381 gaaaggtgcg cttgaagtgg cgcgggaagc agcctgccgg ctgcgggata tgggctggac
    13441 gccggaacgg attcaggagg caaccggact ttccggtgaa gaactgaaaa agctgtttcc
    13501 tgatgagcag tagcctggca ttctgccagg ctgtgaatca cgctcacaca ttccctgccg
    13561 tttcccccag ttcattttcc agacgcctga tgatatctgc cttgcgcatc ccctgcatgt
    13621 gaggcagcag atcacggact gaaagtgcct gggtggtcct ccagacggtg acatccacct
    13681 gttccgcatt ttcccaggcg tgccatgttc tctgtgtcac gccaaattct ctggccgctt
    13741 tttcgcgtga ccagagcatg ctttttcgcc agattcgcag ttcccagccg gtcataaaac
    13801 acctttgaaa aagtgaaaaa acttcacttt attttatcat cataatcacc aggtcagcgc
    13861 gataaataag agtgaaaaag tgaagaaatt tcacttttta gttgtaggtg tcgtgctgtt
    13921 ttctctgttt tcacctgtgc gggagtggtg cgccagtgac caccacatgc cattgttgtg
    13981 ctgtcgtttt tcttcagtcg cgccggatgc ggcgctccct gcacacctga cggtgtgtaa
    14041 ggccactgcg cctgatggcg cagctgtctc tgcctgtctg ccgccgcact gcggcccgtt
    14101 ctccgcagac ggactttccg ggggtacata cacgaccaca tcccgtttca gtcgtccagg
    14161 ggagagatgt accagccaca aaactttttg ccgcaggcgt caaaaacttt tgcgtctgcc
    14221 cccggacaac tgccccgtga cgtggtgagc gtcccccatc ggaaaggtcc actgcgggaa
    14281 gaaccccctg tgaagtggac ggcagaagga gcttcgctcc tccgggaaat gaccaggctg
    14341 acccggcagc cgtgacgatg aggagacaat atgatgaatc agactttacc cactgctgac
    14401 ctgaatactg ccggcacgac agatattatt ccgtctatgg ctatcgaccg catcatcgcg
    14461 cagcgtaacg aaggtattgc actgttcatg caggcgatgg aatgcctggc gacagcgcgc
    14521 aggattctgc tcgatgcttc cggtgacatt tttctctacg ggtttgaaga ctgcgtgact
    14581 gactccgttc gatgcatgga taaaccggaa gaagcaaaaa agaatatcac ccgtcttgcc
    14641 gaccggaaaa tctgggatcg cctgatgact tatacgggca tgtacacctt catgagttca
    14701 tgccagcgtg atgagtggaa cagccagctg atgagcgaca cctgtcctga aatcaccctg
    14761 gacaatgtac tggcaacatt ccgccatctg aatgccagta agatgcagac atttgaacag
    14821 gaactgattg atatctggcg gaaattgtca tgggattaca gaaccaataa tccctgccgt
    14881 ctgggcaaga gaatcattat tgaaaacctg ctgtaccgct ggagtaacgg gcgtgtgacg
    14941 ctggactgca gcggacggga ggcactggat gacctggtac gtccgtttta tctgctggag
    15001 ggacgcaacg ttcctgactt caggaacagc atcggggcgc agtatggtga atttctcggg
    15061 aacggcgaca atatcggtga gttgtttgaa ggggaatatt ttacggtgcg tggctaccag
    15121 aaagggaccg tacacattgt ctttaagcgt cctgaccttg ttgaaaaact gaatgatatt
    15181 attgcacggc attatcctgg tgtattgccg ccacgagtct gattaaacag aaaagccctg
    15241 gtatttatgc cggggctttt ttttctgtaa tcatcataat ctcagaatac gatgtgacat
    15301 tgtcacatca attgttgttc atatcgatgc catctttcct gcctgaatct gtctttttag
    15361 gtgctgtttt agaggaagaa cggcgggatg gttttttcga ttctgcttcc cgaataatgt
    15421 cacgaagtgc cttctctcct acttcatacc ctgcattttt gagcgtgtca cgaacatctg
    15481 ccagtgtata accctttgtc cttaccagga caatgatgtc atcacgaatg gcatcaagaa
    15541 aatcacgcaa tgtttttcgt tgagcggtga ggtccggcaa ttctgataaa gcagcttttg
    15601 ccagctggat gtcatcatct gagtaaaatt ttttagaagc cattggcact ttttctccgt
    15661 ttcatgttga agtctgattt tatcatcaaa aactgcctcc tggcagagct gcgccccgcc
    15721 tctgccgtct gcataagact atgatgcaca aaaataacag gctataatgc ctgaaaaacg
    15781 ggacaggatg tgtaatcgta taaccgcgca cgcacggcta tacgattacc acctggtcag
    15841 ggcttagccc cgacaccccg gtaaggagca gaccatgagt gagaagaaaa cccgaagtgg
    15901 cagtgagaaa cggcaaaaga atgtcctcat tgctgttcgt ttttcaccag aagaggctga
    15961 gattgttaaa gaaaaggcag aaaaaaacgg actgacagtt tcaactctga taagaaaaac
    16021 tgtgctggga aaacagataa atgcccgcat tgacgaagat tttttgaaag agctgatgcg
    16081 tcttggtcgc ctgcagaaac atctgtttgt ggaaggaaaa cgcacgggag ataaagagta
    16141 tgcagaggtt ctcgttgcta ttacagagct tgctaatact ctgcgaagag atttaatggg
    16201 acgctgagat aatcgctgat gaatgccatc attccgaaaa aaagacgcga cggaaagtct
    16261 tcttttgaag acctgattgc gtacacctca gtgcgggatg atgtgccgga agatgaactc
    16321 aggccagaca cggaaatgaa agctgagatt ccgcaccgga accgcttcag acgtcttgtt
    16381 gattatgcaa cacgtctgcg taatgaaaaa tttgtttccc tgattgatgt gatgaaagac
    16441 ggcagtcagt gggtgaattt ctatggtgtg acctgctttc ataactgttt gtctcttgaa
    16501 tctgcggctg aagaaatgca gaaagcagca gaccttgctc atttttccaa agatgataca
    16561 gatcctgttt ttcactatat actctcctgg cctgcgcacg aaagtccgcg ttctgagcag
    16621 ctttttgact gtgtgcgtca caccctgaaa tcccttgagc tgtcaaaaca tcagtatgtg
    16681 gcagctgttc atacagacac ggataacctg catgtacatg ttgcagtgaa tcgtgtccac
    16741 cctgaaaccg gctatatcaa ttgccttccg tggagccagg aaaaactcag tcgtgcatgc
    16801 cgtgaacttg aaattaagca cggattcgcg ccggataatg gctgctttgt gcatgcaccg
    16861 ggcaatcgta ttgtgcgcaa aacagcgctg gtacgtgagc gccgtaatgc ctggcgacgc
    16921 gggaaaaagc agacgttcag ggaatatatt gttcagatgt cgattgccgg gctgcgtgaa
    16981 gagcccgcac aggactggtt atctttgcat aaacggctgg ccagtgatgg tctgtatatc
    17041 acgatgcagg aaggggaact ggtggtgaag gatggctggg acagagcgcg ggaaggtgtt
    17101 gcactcagtt cgttcggacc gttatggaca gctgaaaaac tggggcggaa actgggggaa
    17161 tatcagcctg taccgacaga tattttcagt caggtgggga cacccggtcg ttatgatccg
    17221 gaagccataa acgttgatat ccggccggaa aaagtggcag aaacggagag tctgaaacag
    17281 tacgcctgcc gtcattttgc cgaacgctta ccggaaatgg cgagaaatgg tgagctggaa
    17341 agttgcctgg atgtgcacag aactctggca acggctggat tatggatggg gatccagcac
    17401 gggcatctgg tactgcatga tggatttgat aaacaacaga caccagtacg tgcagacagt
    17461 gtgtggccgt taatgacgct ggattatatg caggatctgg atggtggatg gcagcctgta
    17521 ccgaaggata tttttactca ggttataccc ggagaacggt tccggggacg taatcttggt
    17581 actcaggctg tcagtgatta cgaatggtat cgtatgcgga tggggacagg tccacaggga
    17641 gcaataaagc gcgaactgtt ttctgataag gaaagtctgt ggggatacgg ccttattcag
    17701 tgcaggagcc tgattgaaga gatgatagct gacggggatt tcagctggca ggcatgtcat
    17761 gagatgtttg cgcgtaaagg gcttatgctg cagaaacagc atcatggtct tgtgattgtg
    17821 gatgcgttta atcatgagct gacaccggtg aaggccagca gtatccatcc tgatttgacg
    17881 ttatcccgtg ccgagcctca ggccgggcca tttgaaattg cggctgcaga tattttcgaa
    17941 cgggtgaagc ctgaatgtcg ttacaatccg gaacttgctg ccagtgatga agtggaacca
    18001 ggcttccggc gcgatcctga gattcgtcgt gagcgccgtg aagcccgtgc agctgcgcgg
    18061 gaagatttgc gtgcgcgtta tcttgcatgg aaggagcact ggcgtaaacc cgacttacgt
    18121 tacggagaac ggttacggga aattcatgct gcctgccgtc ggcgcaaggc gtatatccgt
    18181 gtgcagttcc gggatccaca attgcgtaag ctgcattacc atattgcgga agtgcagcgt
    18241 atgcaggcgc tgatcaggct gaaggagagt gtgaaggaag agcggttgtc actgattgcg
    18301 gagggtaagt ggtatccttt gtcctaccgg cagtgggtgg aacaacaggc ggctcaggga
    18361 gacagggctg cagtatcgca gttacgtggc tgggattatc gtgatcgtcg ctcgcgtaat
    18421 aaggataaaa gacgcacaac taatgttgat cgttgtgtcg tactctgcga acccggagga
    18481 acgccacttt ttaataatgt tgcaaaactt gaagctcgtc ttcagaaaaa tggaagtgtt
    18541 catttcaggg atacgcggac aggaaaaaat gtctgtacgg attacggaga tcgggttgtg
    18601 ttctaccatc acacggaccg aaatgaactg gcagagaagc ttgatttaat tgcgccagtt
    18661 ctgttcagcc gcaacggaaa gcttggtttt gagccggaag gttcatacca gcagtttaat
    18721 gatgtgtttg cagaaatggt ggcctggcac aatgcagcgg gaataaccgg aaacggacac
    18781 ttcaccatta ctcgtccgga cgtggatttg caccgtcaac gcagtgagca gtattaccgg
    18841 gaatatatca gacagcagac ccgtttatcg gtgagccatg atgataatta tacgttacgg
    18901 caggagaaaa catgggagcc accatcacct ggaatgtaag cccggataag tgatggtgca
    18961 aagattcagc ccaaaaacgg caggaacctt cgtcaataag ggcgggagta gtcactgaat
    19021 tgagtttata cccggttaac tctgaattaa gggccgtgtc tgcggccctt atccagtgcg
    19081 ccgtaaaacc ccggacttag tgaacagggt tttacagtct gattgacaga tatggtcacc
    19141 agaaaatatt tgtattcctt cagtattttt cgtcagttgt tggttgtgct ccacatgatt
    19201 tctggggatg atcattattt attgtctgcg atttattatt ttcagcgacc ggtaacagta
    19261 tctccagggg gagcagggta ttcagaatcg ccttcactga aggtatatct tcaggatcag
    19321 agtgttcctc ttccggacga ttcagtgtga tataacatac ccggcgttct tccctggttg
    19381 tgttattgat agtgtgcaca gcctttttcc acagttcgtt tgcccgggat tcgatatctg
    19441 tatcatattg cg
//
LOCUS       DAZFKM010000094        19183 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFKM010000094 DAZFKM010000000
VERSION     DAZFKM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..1199)
                     /locus_tag="ACHKAY_003408"
                     /pseudo
     CDS             complement(84..1199)
                     /locus_tag="ACHKAY_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1779..2780)
                     /locus_tag="ACHKAY_003409"
     CDS             complement(1779..2780)
                     /locus_tag="ACHKAY_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5702025.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            2887..3183
                     /locus_tag="ACHKAY_003410"
     CDS             2887..3183
                     /locus_tag="ACHKAY_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5702026.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(3317..3742)
                     /gene="arsC"
                     /locus_tag="ACHKAY_003411"
     CDS             complement(3317..3742)
                     /gene="arsC"
                     /locus_tag="ACHKAY_003411"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO5702027.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(3755..5044)
                     /gene="arsB"
                     /locus_tag="ACHKAY_003412"
     CDS             complement(3755..5044)
                     /gene="arsB"
                     /locus_tag="ACHKAY_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO5702028.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(5098..5451)
                     /gene="arsR"
                     /locus_tag="ACHKAY_003413"
     CDS             complement(5098..5451)
                     /gene="arsR"
                     /locus_tag="ACHKAY_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO5702029.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            5960..6043
                     /gene="dinQ"
                     /locus_tag="ACHKAY_003414"
     CDS             5960..6043
                     /gene="dinQ"
                     /locus_tag="ACHKAY_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO5702030.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(6097..7449)
                     /gene="gorA"
                     /locus_tag="ACHKAY_003415"
     CDS             complement(6097..7449)
                     /gene="gorA"
                     /locus_tag="ACHKAY_003415"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO5702031.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(7521..8363)
                     /gene="rlmJ"
                     /locus_tag="ACHKAY_003416"
     CDS             complement(7521..8363)
                     /gene="rlmJ"
                     /locus_tag="ACHKAY_003416"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO5702032.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            8566..10608
                     /gene="prlC"
                     /locus_tag="ACHKAY_003417"
     CDS             8566..10608
                     /gene="prlC"
                     /locus_tag="ACHKAY_003417"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO5702033.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10616..11368
                     /gene="rsmJ"
                     /locus_tag="ACHKAY_003418"
     CDS             10616..11368
                     /gene="rsmJ"
                     /locus_tag="ACHKAY_003418"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO5702034.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(11417..12886)
                     /gene="dtpB"
                     /locus_tag="ACHKAY_003419"
     CDS             complement(11417..12886)
                     /gene="dtpB"
                     /locus_tag="ACHKAY_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO5702035.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            13052..13105
                     /locus_tag="ACHKAY_003420"
     CDS             13052..13105
                     /locus_tag="ACHKAY_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702036.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(13204..13638)
                     /gene="uspA"
                     /locus_tag="ACHKAY_003421"
     CDS             complement(13204..13638)
                     /gene="uspA"
                     /locus_tag="ACHKAY_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO5702037.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            14029..14364
                     /gene="uspB"
                     /locus_tag="ACHKAY_003422"
     CDS             14029..14364
                     /gene="uspB"
                     /locus_tag="ACHKAY_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO5702038.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(14609..16108)
                     /gene="pitA"
                     /locus_tag="ACHKAY_003423"
     CDS             complement(14609..16108)
                     /gene="pitA"
                     /locus_tag="ACHKAY_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO5702039.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            16340..17542
                     /gene="yhiN"
                     /locus_tag="ACHKAY_003424"
     CDS             16340..17542
                     /gene="yhiN"
                     /locus_tag="ACHKAY_003424"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO5702040.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(17858..18910)
                     /gene="yhiM"
                     /locus_tag="ACHKAY_003425"
     CDS             complement(17858..18910)
                     /gene="yhiM"
                     /locus_tag="ACHKAY_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO5702041.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         5034 a         4660 c         4806 g         4683 t
ORIGIN      
        1 atttgataag cgaattcttt tgccagatgg tatttctatt tccaccccat aatttatatc
       61 tttttcatta ggaagtggcc tctttaaata aggcgtgtgt gacatttttt tggggttaag
      121 cgtctcgtat tgaaatactt cgaactgata ttcagacatt tctgcccatg tttgaatttt
      181 attgtatcga gagagtgtcg taaaggcttt aacttgctta taacattgtt tttggaattc
      241 ttgctgatat gttaatatgt cattgtatga aagattggtt atcctggcct ctaaaaattt
      301 aattattgtc gaagcaattt ccgcttttgc tcgtaaaaag ttgtcattaa tggttaataa
      361 tgttgaaaat aaaccttcga tttgtttgtc aagatattgt ccatctttat ataatttggg
      421 ggtagggtgt tctttatgta aaaaatagtg tcacctaaat tttcgtcagt gaaatttata
      481 ttaagtagat cttcgaaata aaaccttgac aaggttagaa cgaacccgga caggttctgt
      541 cctgtcatat tgaaatcaat aaagttggtt ccggcaaaga ctttcatcct gtttttattt
      601 tcactgtgct caatatataa cttcgtcatt ttttgataca ggctttgaat atcttttata
      661 ttagttggta agtcagctcg ttgttcagca ataaaattag tatctgaaaa catggtttgg
      721 acactgaatg ttatgggtat tccctcttct acgctaattt tagttatgat tgccttgtcg
      781 ggtttaggat tagcgacaac aatgattcga cattcagcac ttttgttttt ttcatatcta
      841 atgttattaa tctgattaag tattgattgt gtctgttgcg taagttgtgt cgaacttatc
      901 gtttcaatgt tagtaataag acttacaagt tgagccacaa cttttgagta gtctgcattt
      961 gtatatgtag tgttcgtgtg gcacgcatta atttgttgaa gttggtgttg cgctaaatca
     1021 tattgatcag agatatatcc ttctccagag aatgtggaat taatgggtta ttgttcttcc
     1081 caattatcgc aaggccactt cggtgtgtct ggcgttttag cattatcggt atttgttgtg
     1141 cagttataaa tatcgccgtg atatgtattt attatacctg gggtaaagtc aattgacata
     1201 aaattcctta ttcatcattt gttttatgct aagatgttta aattggataa ttagaacagg
     1261 aatggatttt cttctacaca caataaatat aatgcgttat atcttcctga tataattacc
     1321 tttaaattcc ctttcatttc cacccggtaa tatttgtgta taaaaaaagg cagtgatagt
     1381 atcgtaaagg gaaacgttaa caactatcaa aaagcgctca ttttaaaaag ttattttgtt
     1441 ctccgatagt gtgtgaagta atatttttgt gatgttttag atgtgtaata atgaaccagg
     1501 ttaaagtgta tttaaaaatg tgttgtatta gcgactgaaa tctgcctttt aaaaaggagg
     1561 agggtactga gacaaagagt cattgcttct ccgataaaat gtcagagaag catgattacg
     1621 tggttattgt ctgttgatga cttgttgacc gagccgtcat cattctcaat gcattagtta
     1681 aatcatttgt gatttttaag gattatcctg tctatgattt tctacttaga aggatatggc
     1741 actaactatg catcttatag cttactgctg gcatctattt aaaatagcaa ttgagtcaaa
     1801 tagcccatca agatagccat acctaatata accgtcagga atgcagctat catcggcatt
     1861 ctgaacattg atttaagtaa tatcacttcc gtcaggcttg cgccggcact gccgatgatt
     1921 aatgccatta atgctcccag acccattcct tttgtcatca gaacagatgc cagagggata
     1981 actgcctcag cccggatata cagcggaata ccgacaacgg cgcttaatgg gatggcgaag
     2041 ggattatctg ctcctgcatg agcggcaatc cacgctgagg gaataaagcc ataaataaaa
     2101 gatcctatta atactcctaa caaaagatat ggcagtacat ctttaaactg ttgcaacgca
     2161 tctttggtgg ctagttttat gggattaata cttactacag ctttggtact gcaacaggta
     2221 actacggggt tctcaatggc tttagccgct ggactacaac aactcacttt tatgggcgta
     2281 tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat ttgctgatga gcttttactg
     2341 gcaatgatat gacgttcaaa ccccagagaa tccaggataa tactggcaag aacggagacg
     2401 ccggctgcga taatcgcgta caacaaggtg actttccaac caaaggtcat ccacattagt
     2461 ccgacgataa tgggattaag taatggtgaa acaaataaaa aagtgagagt cggaccaaac
     2521 ccggctttcg ctgacaacaa tccacgtaac atgggtattg tcgagcaact acagaacggg
     2581 gtaacggctc ccaacagagc agccaggaga tagcctctcc cttttctggc ccccatcatc
     2641 tgctggattt tatggtctgg caccttttgt cttatcaggc tgacaccggc actaatcact
     2701 ataaacagca acgaaagctc gacggcgaga aacacaaaca tctctgcggc atcttgcagc
     2761 atcgcaagcc aactactcat acgtaacctc attggataaa tctatatttc cagaataatc
     2821 gaaatgaaat tcagatcaag atatttctgg tattatcgaa atatcaacca gacagagggg
     2881 tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag agctaggtca cccgacccgt
     2941 ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag ggttgccggt cggtgagttg
     3001 caaaagcagc ttgggatacc tggctccaca ctcagccacc atatatcagc actggtatcg
     3061 gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga tgtgtgtttc gcaatacgca
     3121 atgctggaag cgataattga attcttacgc gaggagtgct gcgtaaatag caaaattgcc
     3181 tgatgcgcta cgctctacat atttcatgcc atatattgaa tttgcacggt tttgtaggtc
     3241 ggataagacg cgttagcgtc gcatccgaca taaacaaagc gcactttacc aactatcggc
     3301 agtggatatc gccgctttat ttcaggcgct tacccgcttc atcaaccact ttctcgccat
     3361 cttccttgga gaatgcgcct ttttgcgcat ctggcagaat ttccagcacc acttctgaag
     3421 ggcggcacag gcgagttccc agcggcgtca ccacaatcgg gcgattaatc agaatcgggt
     3481 gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt atcttccgca aggcccagct
     3541 cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac ggaaatcccc atatcggcaa
     3601 tgagtttgac cagttcatcg cgcgttggcg gagtttccag ataatggata atggtcggtt
     3661 ctgtgccgct gttgcggatc atctccagcg tattacgcga cgtgccgcag gccgggttgt
     3721 gataaatggt aatgttgctc atatcagtat ctcattacaa agtgaaagag agacgtagcg
     3781 ccagcgcagc cagcgtcaca aacagcacag gcagggtcat gataatccct gtacggaagt
     3841 aatatcccca gctgatagtc atattcttct gcgaaagtac gtgcagccag agtagcgtag
     3901 ccaggctacc aattggggta attttcggtc ccaaatcgca gccaatcaca ttggcataaa
     3961 ccatcgcttc tttgataacg ccagatgccg tgctgccatc aatggacaac gcgccaacca
     4021 gtaccgtcgg catattgttc ataatagaag agaggaaggc ggtgaggaat ccggtgccga
     4081 gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag tacgccagaa agatattccg
     4141 ttaatccggc attgcgcagg ccataaacca ccagatacat gccgagcgag aagatgacaa
     4201 tctgccaggg ggcaccgcgc aggactttac ccgtattaat cgcatgaccg cgtttagcga
     4261 cgacaaataa tatcagcgcg cccacagctg caatggcgct caccggaatg ccgagcggtt
     4321 ccaggacgaa aaatcccacc agcagaagca gtaaaacaac ccagccagtt ttgaacgtag
     4381 caggatcttt gatcgcttct gcgggagatt tcagcagcgc catatcgtag ttctgcggaa
     4441 tatctttgcg aaaatagaga tgtaacatca ccagcgtggc aacaatcgcg gcgatatcca
     4501 ccggcaccat caccgaggcg tattcgcgaa agccgaggcc aaagaaatca gcggaaacga
     4561 tattcaccag gttggagaca ataagcggca ggctggcggt atcggcaatg aatccggccg
     4621 ccatcacgaa cgccagcgta gtgcctttac tgaaccctaa agccagcagc atggcgatga
     4681 caatcggtgt caaaataagc gccgcgccat cattggcaaa cagggcggca acggcagcac
     4741 cgagcaggac aatccaggta aacagcaagc gaccacgacc attaccccag cgtgagacgt
     4801 gcagcgccgc ccattcaaaa aagccggact catccagcag caggctgatg ataatgacgg
     4861 cgataaacgc agccgtcgcg ttccagacga tattccacac caccggaata tcacccggat
     4921 ggaccacgcc cgtaactaac gccagtactg cgccgagcgt tgcactccag ccgatgccta
     4981 aacctttcgg ctgccagata accaatacga tggtcaggac aaagatagcg cctgccagta
     5041 acataatgcc tcccggataa aacacatctg aaaattcata tgtgtttagc taaattttta
     5101 actgcaaatg ttcttactgt ccccggaaca gttttgtcga gccaggttgc ggacaatcgc
     5161 ctgaaccttt tcctgttcac atcgccaggc ctcatcaata attttcgccg cccatgctgg
     5221 aatatgcggt gataagcggt aatgaaccca cttaccttgc ttgcggtcca gcaatagccc
     5281 gctttcacgc agcaatgcca ggtggcggga gatcttgggc tgcgactggt cgagagcagt
     5341 gcagagatcg cagacgcata actctcccag ttcgctgagc agtaaaacga tgcccagacg
     5401 ggtttcatca gcaagaattt tgaacaattg gatgggtaac agaaatgaca tattgcgctc
     5461 ctgattgttg caggtagtgt ctctcttcga agcggataag tcaaaaacat atatgactta
     5521 acgaatgtgt aagtgcagag gaaggtaata ggtgtgaatt ttgagttggc tattcatttg
     5581 aaaggaggtc tgaatcagga cgtaaaaaaa ccgactttgc gtcggttttt tactttccag
     5641 ccctgagttg gtggcactgg ctggagtgag aatgccatta tctaacgcat caatgctaat
     5701 accatactga aactattgca aggacatgct ggttttataa cctgaatgta ctgtatgatt
     5761 atccagttag ctctgaggca ttttcactct ggtaatgcgc ataaacgctt tcaaagtcct
     5821 ggtcagaagt acgggtggtg ccgttaactg atgctctggc cggagtgaga atgccattat
     5881 ctaacaatag ggcatgcgcc gtgacaggca gtggatgagt aagcggatgc attctcactc
     5941 catcgcatgg agaaaacggg tgattgataa agcaatcatc gttcttgggg cgttaattgc
     6001 gctgctggaa ctgatccgct ttctgcttca gcttctgaac tgatagcgga aacgtaatta
     6061 agggctaaga gcacactact cttagccctt taacatttaa cgcattgtca cgaactcttc
     6121 tgccgccgtt gggtgaatgg cgacggtatt gtcgaagtct tttttggttg cacccatctt
     6181 cagcgccacc gcgaagccct gcaacatttc gtccatacca aagccaatgc cgtgaatacc
     6241 gacaatcttc tcttccgatc caacgcacac cagcttcatg cggcacggct ggcggtgagt
     6301 ggtgacggcg gtatacatcg cggtgaaaga ggatttatac actttcacct gatcgtcgcc
     6361 atactgctcg cgcgcctgcg gttccgttaa accaacagta ccaatcggcg gatggctgaa
     6421 gaccacggtc ggaatgttgc tgtaatccag atgctcatcc ggcttgttat taaacaggcg
     6481 ttcagagaga cggcgacccg ctgcaactgc caccggtgtc agctccactg cacccgtgtt
     6541 atcgcccacc gcgtaaatac cttcaatatt ggtgttttga tatttatcga cgacgatata
     6601 gcctttttcg ttagttttaa cgccagcggc ttccaggttg atgttgtcat tggcaggctc
     6661 gcgaccaatc gcccaaatca ggcaatccac cgtttcactg cgaccatctt ccagctccag
     6721 cgtcaggcta ccatcggcat ttttcactac cgctttcggg atggcgttgg tgtgcagctg
     6781 cgggccttcg gcgttcatca cttcgaccag cgtttcggaa atcatcgggt cgaagctgcg
     6841 cagcggcgca tgtttacgca caaacagatg cgttttcgcg ccgaggccgt taatcacgcc
     6901 cgccagctca acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg
     6961 aagggcgaag aagccatcag aatcaatacc gtattccacg cccggaatat ccgggtggct
     7021 cggacgaccg cctgtggcga tcagaatatg atcggccgtg atggtttcgc cgtttacctc
     7081 cagcgttttg gcatcaacga agcgggcaaa gcctttgatt acatcaacgt tatttttacc
     7141 gagcacgttt tcataggaag tatgaatacg gtcgatatag gcggtacggc tggcgatcaa
     7201 cgtttcccag ttgaatttat tgatagtggt atcaaaacca taatccgggc cgtacatatg
     7261 gatcgcttca cggatttgcg ccgcgtgcca catcactttt ttcggcacac agccaacatt
     7321 tacgcaggtg ccgcccagct ctttggcttc aatcagcgca catttctggc cgtacatagc
     7381 cgcgcggttg atggaggcga taccgccgct gccgccgccg atggcgatgt aatcatagtg
     7441 tttagtcatg acaaagtgtc cttatcgttg attaccgcga ttgtagcgcg agacgtaata
     7501 ggtgccagca atggctgcaa ttactccggc acgatccagc ttacggtggc gtgcccggtg
     7561 cctgccggaa ccagtttgct gtgcagccac ggcagcacgt tattcatctg ctgttccagt
     7621 ttccacggcg ggttaatcac aatcatgccg gaagcggtca tgccacggcg atcgctgtct
     7681 ggcagtaccg ccagttcaat ttgcagaatt ttgcgaatac cggtcgcttc cagatcgtgg
     7741 atcatgcgct taatttgctg acgcagcacc accggatacc acagtgcgta agtaccagtg
     7801 gcgaaacgtt tgtaaccttc tgctatcccg ctgaccaccg cctgatagtc ggttttcatt
     7861 tcatacggcg ggtcgataag gattaaacca cggcgggaaa ccggcggcag tttggcctta
     7921 agctgctgga aaccatcggc tttttcgacg cgcgcacggc tgtctttctg aaattcagaa
     7981 cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc ttgttcgcgc
     8041 agtagctggc gagcaatcaa tggcgaaccg gggtagtaac gcaactgacc gctacggttg
     8101 aagtgtttta ccacattgat gtacgcctcc agttctgcgg gcaaatcgtc ctgctgccag
     8161 atacgggcga tgccttcgag atattcgccg gtacgctcgg catgttcgct gcctaactga
     8221 taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc
     8281 gactcgatga tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt gccagcgtga
     8341 aagctgtggc gataactgag catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta
     8401 ttcggggcgc acaaaagcgt atcaggacag tataccgaaa agaggccgcc gccgcgaaag
     8461 cgtaacgttt ctcattgaaa ttcactacac ttaaccccat gctacacaca ttatgtaaag
     8521 cgcctgttga gcgcttcctt aacctcttta accaggactg cgcgaatgac gaatccgtta
     8581 ctgactccct ttgaattgcc tccgttttct aaaattctcc cggaacatgt cgttccagcc
     8641 gtgactaagg cgctgaacga ctgccgcgaa aatgtggagc gcgtagtagc gcaaggagca
     8701 ccgtacacct gggaaaatct ctgccagccg ttggcggaag tggacgatgt gctggggcgt
     8761 atcttctccc cggtcagcca cctgaactcg gtgaaaaata gcccggaact gcgtgaagcg
     8821 tacgaacaaa ccctgccgct gctgtcggaa tacagcacct gggtagggca acatgaaggg
     8881 ctgtataaag cgtatcgcga cctgcgcgat ggcgatcatt acgccacgct gaacacggct
     8941 cagaaaaaag cggttgataa cgcactgcgc gacttcgaac tctctggcat cggtctgcca
     9001 atagagaaac aacaacgcta cggtgagatt gccactcgtc tttccgagct gggcaaccag
     9061 tacagcaaca acgtcctcga tgcgacgatg ggctggacca aactcgttac cgacgaagcg
     9121 gagctggcgg ggatgcctga aagcgcgctg gctgcggcaa aagcccaggc cgaagcgaaa
     9181 gagctggaag gctacctgct gacgctggat atcccaagct acctgccggt aatgacctac
     9241 tgcgacaacc aggccttgcg tgaagagatg tatcgcgctt acagcacccg cgcctccgat
     9301 caaggcccga acgccggtaa atgggataac agcaaggtga tggaagagat cctcgctctg
     9361 cgtcacgaac tggcgcaact gctgggcttt gaaaactacg cctttaaatc ccttgccact
     9421 aaaatggcag aaaacccgca gcaggtgctg gatttcttaa ccgatctggc aaaacgcgcg
     9481 cgtccgcaag gcgaaaaaga gctggcacaa ctgcgcgcct tcgccaaagc ggaatttggc
     9541 gtcgatgagt tgcagccgtg ggatatcgcg tactacagcg aaaaacaaaa acagcacctc
     9601 tacagcatca gcgacgagca gctgcgtccg tacttcccag aaaacaaagc ggttaacggc
     9661 ctgtttgaag tggtgaaacg tatttacggc atcaccgcta aagagcgtaa agatgttgat
     9721 gtctggcatc cggatgtacg tttcttcgaa ctgtatgacg aaaataacga actgcgcggc
     9781 agcttctacc tcgatctgta tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac
     9841 tgcgtaggcc agatgcgtaa agctgatggt tcgctgcaaa aaccggtcgc gtatttgact
     9901 tgtaacttca accgcccggt aaatggtaaa ccggcgctgt tcactcacga ggaagtgatc
     9961 accctgttcc acgagttcgg tcacggcctg caccatatgc tgacccgcat cgaaaccgct
    10021 ggtgtttccg gtatcagcgg tgtgccgtgg gatgcggtcg aactgccgag tcagtttatg
    10081 gaaaactggt gctgggagcc ggaggcgctg gcgtttatct ccggtcacta tgaaaccggc
    10141 gaaccgctgc cgaaagagtt gctggataaa atgctggcgg cgaagaacta ccaggcggcg
    10201 ctgtttattc tgcgtcagct ggagttcggc ctgtttgatt tccgccttca tgccgagttc
    10261 cgcccggatc agggggcaaa aatcctcgaa actctggcag aaatcaagaa actggttgcc
    10321 gtggtgccat ctccgtcctg gggccgtttc ccgcacgctt tcagccatat tttcgccggt
    10381 ggttatgcgg caggttacta cagctacctg tgggctgacg tactggcggc agatgctttc
    10441 tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt cctcgacaac
    10501 attctgagcc gtggcggttc agaagagccg atggatctgt tcaaacgctt ccgtggtcgt
    10561 gaaccgcagc tggatgcgat gctggagcat tacggcatta agggctgatc attcagtgaa
    10621 aatctgctta attgatgaaa caggcgccgg agacggcgcc ttatctgttc tggcggcccg
    10681 ctgggggctg gagcacgatg aagacaacct gatggcgctg gtgttaacgc cggaacatct
    10741 ggaattgcgc aagcgtgatg agccaaaact tggcggcata tttgttgatt ttgttggcgg
    10801 agcgatggcg caccgacgca aattcggcgg tggtcgcggt gaggcggtgg cgaaagcggt
    10861 gggcattaaa ggcgattatt tgccggatgt ggtggacgcc accgcagggc tggggcgcga
    10921 tgcctttgtg ctggcttcag tcggttgccg cgtgcggatg ctggagcgta atccagtcgt
    10981 tgccgcattg ctcgacgacg gcctggcgcg tggttatgcg gatgcggaaa tcggcggctg
    11041 gttgcaggag cgtttgcagt taattcacgc ctccagcctg acggcgctaa ccgatattac
    11101 cccacgcccg caggtggtct atctcgaccc gatgttcccg cataagcaga aaagcgcgct
    11161 ggtgaaaaaa gagatgcgtg tctttcagtc gctggtgggg ccggatcttg atgccgatgg
    11221 attgctggag cctgcacgct tgctggcgac caaacgcgtg gtagtaaagc gcccggacta
    11281 cgcgccaccg ctagcgaatg tcgccacgcc aaacgcggtt gtgactaaag ggcatcgctt
    11341 tgatatctat gcaggtacgc ccgtgtaaaa gtaaagcagg gtagcgataa cgctaccctg
    11401 ctttctgcaa gaataattaa tggctttccg gcgtcgcaat catgcgtttc agccacggca
    11461 ccatcaacag catcactact gcaacgccca gcgtgaccag accaatctta ccaaacacgt
    11521 tggtatagac gggcaacgtc tcaagcggat cggtaatgtt gtccggcacc gcggtaaatg
    11581 ttgccacata gccgcccagc aagaacgcgg cagcctgcgt caggaaccac atcccgagaa
    11641 taaagcccat caaatgctgc ggcaccaggg cagcaatcat cgccaggcca agggcgctaa
    11701 taaacagttc acctaagctc tggaataagt acaccagcac gataaaccat ggcgatgtca
    11761 gcccttgtgc atccgcaaac cacattcccg cagctgccgc cgtcaaaaag cccagtgagc
    11821 acataaacat gccgagagta aatttcatcg gcatcgagag gtctttgcct ttgttaccca
    11881 gatgcgtgta aatgcctgcc agtattgggc tggcgagtac cacccagaac gggttaagcg
    11941 cctggaagct gaccgggttg atggaaaaac cgagaatttc atgatgcacg ttgttgatgg
    12001 caaagaagtt cagcgatgtt ggcatctggg cgtagagaat gtaaaacacc accgcttcga
    12061 gcatcaggac aaaggcgaca aacattttat tgcgcccggt tttatccagc ttgaatgcct
    12121 gacgaaagaa gatgatggtg acgacgatgg agagaacaat cagcaccaga ttggcgactt
    12181 ctacgttgtg catcagccat gcacatacga agatcatcac cacgctgcca agtaatacgt
    12241 acaacagttt gctgaagctc atcggcttga agtcgggttc agaaccaatg tctttcacca
    12301 ttccacgaca ggcgatgtaa accagtaatg cgataattaa ccccgccccg cacaggttgt
    12361 aggtgactga ataaccgaat ctatcagcga tcacaggggc cagcgataac gctatcaacg
    12421 agccgatatt gatcgacata tagaacaggg tgaatgcgcc atcaagccgc ggatctttcg
    12481 gcggatagca cttcgaaagc aagctggctg ggttagcttt aaacaggccg ttaccgacag
    12541 cgatagtccc cagggcgatg aaaatcaggt caggcttaag tagcgacatg ccggtcatga
    12601 agtagccaat cgccagcaca agtgctccga gaacaatggt gcgtttggtc cccagcaggt
    12661 ggtcgccgac atagccgcca atggaaatga ggccatagac cagcgcagca aaagcaccaa
    12721 aagtgacaaa agcctgctct tgcgagaatc caagctgttt aacgaagaaa accgccagta
    12781 cgccctgcac gccgtagtag ccgaatcgct cccataactc gacaaaaaag atcatgaaaa
    12841 atgggcgagg ttgctgcagc atccccatgg gtgttgttgt attcatattt attaaccttc
    12901 caataccatc ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag tttacatctg
    12961 tcagagattc ttaatattga tgcggtgaat agattaatac actattgcaa cagaaaataa
    13021 ccagctgatc acactaatga tggaaaatat gatggtgtta aatgttgtat tggcaggaga
    13081 ttttgattgc gtacgcagat cgtaggccag ataaggggtt tacgctgcat caggtaacaa
    13141 ggcgcgccaa aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt cgtggaggga
    13201 tgattattct tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa cggtgttgat
    13261 cagctgacgt gcggaagaca tcagtttgct ccagaagtcc tggtggtgac cacaaaccac
    13321 cagatccata tcgtatttct tgattgcatc gaccagaacc tggcccaggt cgccgctgcc
    13381 gctcagggtt tcagtgattg ggtagcctgc attagtggaa agctcggtca gcgcatgatg
    13441 tgtctcttca gagatgcgtt tctgcatatc acccagattc acatcaataa gcccggtgta
    13501 taggtcagag tagtttacat ctacgtggat cagagaaact ttcgcattgt aggggcgagc
    13561 catagagact gctttctcta ccagaacttt gctttccggg gagagatcga ctgcgatgag
    13621 aatgtgttta taagccatag tgttactcct tccataaagt tgtcgatgac tggccagcta
    13681 gcgtttcttg tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg ggctaaccca
    13741 gccgtaaaga ttcagtgtta agaccatcct taccttatag cgacccggat gatccgtcaa
    13801 tccgccttgc ttacccgtta gataaacaaa ttttcgacat gaatatattg atagtggtta
    13861 accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc tcggatgttg
    13921 gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc gtatcgggag
    13981 cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat gataagcacc
    14041 gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg ctatttctca
    14101 tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta tcaatatgtt
    14161 gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg tctcgtttgg
    14221 tatatctatg cccaacgtta tcgcgatcat cacgatgatg aatttattcg ccgctgtgag
    14281 cgggtgcgtc ggcagtttat tctgaccagc gcattgtgtg gtctggtggt ggtcagcctg
    14341 attgcattga tgatttggca ttaagcactt cagcaataat aacgcataaa aaaagcgggc
    14401 cagtaaactg acccgctcga gactgattac aaacgcaaaa ttgcctgatg cgctacgctt
    14461 atcaggatct acataatctc tgcaatctat tgaatttgca tgtttttgta ggccggataa
    14521 ggcgttcacg ccgcatccgg catgaacaaa gcgcactatg tcacaatctg aagcgggcca
    14581 gctgacccgc tcgttttggt gtgtacgatt acaggaactg caaggagagc cagtacagtc
    14641 cgccggaaag cagtaccgca gccggaaggg taaacaccca ggccatcaga atactggtta
    14701 cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca gaagaggaga
    14761 gtacgtgagt agtggaaacc ggcatcccgg tataactcgc caggccgata gacactgccg
    14821 ccgtcatctg ggccgacatc ccctgagcgt aggtcatgcc tttcttaccg attttctcac
    14881 cgatagtcgt tgccacacgg cgccagccaa tcatcgtacc gatacctaac gccagcgcga
    14941 ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg gacttcagtt
    15001 tcttcaacag gcgctgatcg tcagcactca caccaggcat cttcaccact ttgtcgatag
    15061 tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc gacagcttgt
    15121 cgtagctttc cacatcggta gtcaacatgc ctttcaggcg gttgagcgcg ttaatggtat
    15181 tcgacggatg gcaatggaac tccgcgggtt gcgttgcgcc agcttccgga gccggtacta
    15241 actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag taagcttcga
    15301 cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc atgttcacca
    15361 cgaagcctgc tggtgcgacg ccaatcaata ccaacataac cagaccaatg cctttctgac
    15421 catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc gcaatacgcg
    15481 tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg gtcaggtgga
    15541 tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc agaccgccag
    15601 caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt aatactttcg
    15661 ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt aaaccaatcc
    15721 cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag taccaggtac
    15781 ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg ccatgagacg
    15841 atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag gcaacactca
    15901 gaccacccag caaaacaccc aaaaagttga acaccgctgc cataaccacg gcgagctgag
    15961 aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca tggaaaccat
    16021 tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc ccggtatgca
    16081 aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg acatgaacgc
    16141 ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt tttttgattt
    16201 gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg caaatacttc
    16261 cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc acgcccgttt
    16321 tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg ctggtgcggc
    16381 gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc tgatcgataa
    16441 tggtaaaaaa ccagggcgca aaatccttat gtctggcggt gggcgctgca actttaccaa
    16501 cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta agtctgcact
    16561 cgcgcgtttt acccagtggg atttcattga tctggtcaat aaacacggca tcgcctggca
    16621 cgagaaaacg ttaggacaac tcttctgcga tgactccgcg cagcagattg tcgacatgct
    16681 ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag tgctgagtgt
    16741 ggcgaaggat gaaacaggct tcacgcttga actgaacggc atgactgtcg gttgcgaaaa
    16801 gctggtcatc gcgaccggtg ggctgtcaat gccggggctg ggcgcgtcgc cgtttggtta
    16861 taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc tggtgccatt
    16921 cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg cggtgccttc
    16981 cgtgattacc gctgaaaacg gcatcgtttt ccgtgagaac ttactcttca cccatcgcgg
    17041 cttgtctgga ccggcggtgt tgcagatttc cagctactgg caaccgggtg aatttgtcag
    17101 tatcaatctg ctaccggatg tggatctcga aaccttcctg aatgagcagc gtaacgcaca
    17161 tccgaatcag agcctgaaaa acacactggc ggttcatcta ccgaagcggt tggttgaacg
    17221 cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg tgcgtgacca
    17281 acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca ctgaaggcta
    17341 tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactctctt cacggacgat
    17401 ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg ttaccggctg
    17461 gctggggggc tataacttcc agtgggcgtg gtcgagtgcg tgggcttgtg cgcaggattt
    17521 gattgcagcg aagtcgtcct gacctgttac ctaaagatga aaattattag agccgcatta
    17581 aaaatgagct aattttgata gtggctatct tgtgattatt ttctaatgag cccgtgaact
    17641 gaaaccctcc aggcttaata taaggtggaa ggaaaggtga ttgaaaactc acccagtggc
    17701 cagatcttta taaaatatga acaggttgaa aaaaacagta actttgttgt ttttttatcc
    17761 ttaaccacta tgcattaatg ctgcgttatt tcatgatgcc taagaaaaac cagcgttacg
    17821 caaatggtca acgctggttt tatccggtac gttgcaatta ttttttagca gaacctgctt
    17881 ctaatattga aacgattgag aacaacgtaa agcataccgc tcctaaaccg accaaaaccc
    17941 gcgaaggaat aaaatatccc atgtcggtct gcgccatttc cgcaagaaac gatgccagga
    18001 aaaggcaaaa caggcaggtg aagacgggaa tcatcggtat tctgttggct aacgaacagg
    18061 tacgtctcca taccagtgcc agtagccaga cttttgagaa tatgctgtaa cagatcattc
    18121 cgaggcaaat aagaataatg ccgggagcca gtcgggcgct tgcatcggaa ctgactaaga
    18181 cgtatatccc ctgcagtacc gtgattgagc cgagaaaaat aacccaataa caccacagcc
    18241 agtgttcttt agttgaaaac gtattacgtg tttgatggac aattgtggca acaaggccaa
    18301 ttagacaggc acagattgcg gttaacccaa gcaatacgtg acccgcgaca taatgcggag
    18361 ttatgtcagc actacgtaac agcgtaatag accaaatgaa accgagcagg gtgagtaaaa
    18421 cgggaacggc aatgaggcaa ttaccgatta atgaagaata agcctgtacc ggcgttccat
    18481 cgctcttgct ccccgctgca tttttgggaa tgagcagaaa gtgaccggat gacgctgcca
    18541 ccgtcgatac acaggcggca atcataccaa cgccgaaaat aacatggccg gcgacaaact
    18601 cgtctgccat cacatcattg cctgctaaca gtgcccaacc ccatatcatg gtgataattg
    18661 accccgcata gccaataatg gggaacaatg tattgtaaaa cgtattaacg ccgcgcgtga
    18721 gctgcgaaat gataataaat gcggtagtga ataatgccag gcatatggct gccagagaaa
    18781 tcagcacatg acccgcaacg aaatactcac tttgcccaga actttccagc acaaagccac
    18841 ctaacgcgat acaaattaag cccataatta aagggattaa tttgaaaagc cacccgatat
    18901 atatgttcac tttaaactcc tcaggtatcc ctgctaatat tgaaatgtgc attaatgata
    18961 atttcagcct gttaatataa tgacgagata tcattaaaga caaatgcata gcataactat
    19021 agcgggattt actttgcaga gcgctacttt tcagcgttca aaaggccatg gtaaataaat
    19081 atttatccta agattaattc ttattctgtt gttttttaca attatcttcc agggagggat
    19141 tctttttcat tatttggtaa aagaccggat ggtatcggtg ctt
//
LOCUS       DAZFKM010000095        18908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFKM010000095 DAZFKM010000000
VERSION     DAZFKM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..114
                     /locus_tag="ACHKAY_003426"
     tRNA            28..114
                     /locus_tag="ACHKAY_003426"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Leu,seq:cag)
     gene            complement(153..389)
                     /gene="yjjZ"
                     /locus_tag="ACHKAY_003427"
     CDS             complement(153..389)
                     /gene="yjjZ"
                     /locus_tag="ACHKAY_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO5702042.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            530..1318
                     /gene="fhuF"
                     /locus_tag="ACHKAY_003428"
     CDS             530..1318
                     /gene="fhuF"
                     /locus_tag="ACHKAY_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO5702043.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1356..2033)
                     /gene="bglJ"
                     /locus_tag="ACHKAY_003429"
     CDS             complement(1356..2033)
                     /gene="bglJ"
                     /locus_tag="ACHKAY_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO5702044.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1991..2716)
                     /gene="yjjQ"
                     /locus_tag="ACHKAY_003430"
     CDS             complement(1991..2716)
                     /gene="yjjQ"
                     /locus_tag="ACHKAY_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5702045.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3335..4105
                     /gene="yjjP"
                     /locus_tag="ACHKAY_003431"
     CDS             3335..4105
                     /gene="yjjP"
                     /locus_tag="ACHKAY_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO5702046.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            4096..4569
                     /gene="yjjB"
                     /locus_tag="ACHKAY_003432"
     CDS             4096..4569
                     /gene="yjjB"
                     /locus_tag="ACHKAY_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO5702047.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4676..5215
                     /gene="dnaT"
                     /locus_tag="ACHKAY_003433"
     CDS             4676..5215
                     /gene="dnaT"
                     /locus_tag="ACHKAY_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO5702048.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5218..5955
                     /gene="dnaC"
                     /locus_tag="ACHKAY_003434"
     CDS             5218..5955
                     /gene="dnaC"
                     /locus_tag="ACHKAY_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO5702049.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            6004..6498
                     /gene="yjjA"
                     /locus_tag="ACHKAY_003435"
     CDS             6004..6498
                     /gene="yjjA"
                     /locus_tag="ACHKAY_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO5702050.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6752..9043
                     /gene="opgB"
                     /locus_tag="ACHKAY_003436"
     CDS             6752..9043
                     /gene="opgB"
                     /locus_tag="ACHKAY_003436"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO5702051.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9182..10204)
                     /gene="lgoD"
                     /locus_tag="ACHKAY_003437"
     CDS             complement(9182..10204)
                     /gene="lgoD"
                     /locus_tag="ACHKAY_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO5702052.1"
                     /translation="MSTMKVLICQEIKNLVWTVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNFHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10343..11248
                     /locus_tag="ACHKAY_003438"
     CDS             10343..11248
                     /locus_tag="ACHKAY_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO5702053.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(11419..13071)
                     /locus_tag="ACHKAY_003439"
     CDS             complement(11419..13071)
                     /locus_tag="ACHKAY_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO5702054.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNL
                     AALNARRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
     gene            13751..14722
                     /locus_tag="ACHKAY_003440"
     CDS             13751..14722
                     /locus_tag="ACHKAY_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO5702055.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            14786..15259
                     /locus_tag="ACHKAY_003441"
     CDS             14786..15259
                     /locus_tag="ACHKAY_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO5702056.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            15256..16527
                     /locus_tag="ACHKAY_003442"
     CDS             15256..16527
                     /locus_tag="ACHKAY_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO5702057.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(16570..18234)
                     /gene="tsr"
                     /locus_tag="ACHKAY_003443"
     CDS             complement(16570..18234)
                     /gene="tsr"
                     /locus_tag="ACHKAY_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO5702058.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(18353..18799)
                     /gene="hpaR"
                     /locus_tag="ACHKAY_003444"
     CDS             complement(18353..18799)
                     /gene="hpaR"
                     /locus_tag="ACHKAY_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO5702059.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         4887 a         4802 c         4403 g         4816 t
ORIGIN      
        1 gcgatatcaa aaaagtaaga tgactgtgcg aaggtggcgg aattggtaga cgcgctagct
       61 tcaggtgtta gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaattatc
      121 tttacttcct ttcttgtttc ttccttgatt tttcatccct gattcaaatt cattagcatc
      181 agcatgatgc cagcaataag cgccaggcac gatggtagca aaacgtaatg ccgtaactgt
      241 ttgtgataca gcatcaccgg cgtcaatatc aatcccatca gaatgacaat ccgccattga
      301 tccggcgata aatccgcata catcagccct gcaatcagca atcccggcag caacactccc
      361 cagccactgc ccagcgtacg ttgcaacatg gtttcatctc tttcattgat aatgataacc
      421 aatatcatat gataattttt atcatttgca agccagagaa atcccttgct atcgggtaaa
      481 cctatcgcta ggattagcaa tcattatcat ttagattact atcccgatta tggcctatcg
      541 ttccgcaccg ctctatgaag atgtcatctg gcgaacgcat ctccagccgc aggatgctgg
      601 gcttgcacaa gccgtacggg cgacgatcgc cgaacatcgt gaacatttgc tggagtttat
      661 ccgtctggat gaacccgccc cgcttcgcgc catgacgctg gcgcaatggt catcaccgaa
      721 tgcgctaagt tctctactgg cggtctattc cgatcatatc tatcgcaacc aaccaacgat
      781 gatccgcgag aacaagccgc tgatctcact atgggcgcaa tggtatatcg gcctgatggt
      841 gccaccatta atgctggcgc tgctgacgca ggaaaaagcg ttagatgtgt cgccggaaca
      901 tttccacgtt gagtttcacg aaaccggccg tgccgcctgt ttctgggtcg atgtgtgtga
      961 agataaaaac gcaacaccac attcgccgca gcagcgaatg gaaacgttaa tcagccaggc
     1021 gctggttccg gttgtgcagg cactagaagc gacgggagag atcaacggca aacttatctg
     1081 gagtaatacc ggttatttga tcaactggta tctgactgag atgaaacagc tgctcggcga
     1141 ggcgacggtt gaatcgctgc gccatgccct cttttttgag aaaacgctta ctaccggtga
     1201 agataatcca ctctggcgta ccgtggtact gcgcgacggc ctgctggtgc gccgcacttg
     1261 ctgccagcgt tatcgcttac cggatgtgca gcaatgtggc gattgtacgc tgaaatgaaa
     1321 gcactgccgg ggaagtaaac ccggcatcat gcggattaat agggatgcaa cacattactt
     1381 gcttcgcaat gccgcataca taacagaata tctgcggcct ccaacaaccc tgcatcagaa
     1441 ctgacgccca gtttcgacat cacattaaat ttatgtgcac ggatcgtttt gatattacgt
     1501 ttaagctgct cggcaatttg tgtcattgag taaccacgcg acataaagcg caatatttct
     1561 ctctccgtcg ggcttagcgt ccggctttgg ttaatgtacc actgattgtt cagtcggtcg
     1621 gtcgcctgac gtacaccatt taatgacaaa aagagttcct gatgaaaaat ctccagcgtt
     1681 gacgctttac ttaatacacc gtccagcggt gatggcgaca atgaaccaat cagccgagct
     1741 tcaatatcat catccgcaat aactaaacgc cgggtacgcg gaagcttaat cgccagttca
     1801 gtcaggcaag ataatccctc tctgcgctca cttctcatgg cagaaaaaga aaaaatgacc
     1861 gccgcaaacg agatccgcgt catggcatcc tgaaatgact cctgactggt ataggtatgg
     1921 agcttataag ggttacccgc aaactttctg aataaactct caataccgat actactcatg
     1981 acgcattttt ctatgagtgc gacatttctc ttcttaattc ggctgtgttc cattctgcgg
     2041 catctccata aacagaaggc aagttaagtt ctcttgcacc ctgcgcgcta tttatccatg
     2101 catacatttc agcgttgctg tgaattgcca gtcgacgcat cgcactgttt ttttgggcgc
     2161 tgatagtttt attacttttc ttaagcaatg aagctatctg gttgatgccc catcctttac
     2221 ctaatagccg cagtaccttg cgctctgaaa gagtgagaat gacggagcga tagctaaaat
     2281 catgagtttc agtcattgcc ggagaggtca gcatggcgct gatacgctct gcgtgcgtat
     2341 tgccggaacg tacggtcttg accagattct caatgggttc aacatcagac aataacgtcg
     2401 ccgtagggca catgagcagt tctactgcct gggaatacca ggagcgtgac accatgaaaa
     2461 cccagtgaat ttcccgatat tgtgagatta aagaataata atgttcacaa acagaacgtg
     2521 gatcttcact ttcaccggct aaatcagcaa tgactaatcc ggaacggcgt aattgtaata
     2581 aggtaaggtc ctccgcagag gcgctggata ttatttcata ttcagggaag tgagtcctca
     2641 tgacctcttt taaccctgct tgcataacag gtattttact gataacaatt ccatttttgc
     2701 agcatcctgg caacatattc cctccacatc cactgtctaa aaattatgat ggtttattca
     2761 ggatattcat tccttaaatg tttccagcaa attaatagat taagcgaata aattatcctt
     2821 agcattcact ttcatatcag catactcctg ctgcacaaca actccactgc atgtgtcaga
     2881 aggttagcat caaatgataa cctggtgaaa tagcctggag acatttaaaa tatctacatt
     2941 aagttgttag gaatacttaa tgaaaaacag aaatcacatt catttaattt ataattaaat
     3001 gatatccctt cctgcagaat gcaaaatcac acaaccaata tttattcaat gaaatctgat
     3061 ggttttaact tgttaacatt aaaaaacgtg cccggttatc tattatcctg attcgtaaag
     3121 gccaaacaat aaattcatta tgatgataat attcatgtga tagtttgttt gcggcgagag
     3181 ataattcgct ttttatcacc gagcttctcc cgataaccag gattaatcag cgcaattttg
     3241 atcttaacct accgcaaaac aaccgtgctt catgatacaa atggattctc attatctgaa
     3301 caatacgcag cacgtctatg acaaagggcg agttatgcaa actgagcaac agcgagccgt
     3361 aacacggctt tgtatccagt gtggattatt tcttttgcaa catggtgcgg aaagcgcgtt
     3421 ggttgatgag ctttcctcac gactgggtcg ggcgctggga atggacagcg tcgaaagttc
     3481 tatctcttcg aacgccatag tgctgacaac cattaaagat gggcaatgcc tgacatcgac
     3541 acgtaaaaat cacgatcgcg gcattaatat gcatgtggtg actgaagtcc agcacattgt
     3601 gattcttgcg gagcatcatc tgctggatta caaaggcgta gagaaacgat ttagccaaat
     3661 tcagccatta cgttacccaa gatggctggt agccttaatg gttggccttt cttgcgcctg
     3721 tttctgtaaa ctcaataacg gtggctggga tggtgccgtc atcaccttct ttgccagtac
     3781 gaccgcgatg tatatccgcc agctgctggc acaacgtcat cttcatccac agatcaactt
     3841 ttgccttacc gctttcgccg ccaccaccat ttccggattg cttttgcaac tccccacttt
     3901 cagcgataca cccaccattg cgatggccgc cagcgttctg ctgctggtgc cgggctttcc
     3961 gttgattaat gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg
     4021 ggcgatcgcc agtctgctga cgctggctac ctgcgtcggc gtggtgatgg cactgacgat
     4081 ttgggggcta cgcggatggg tgtgatcgaa tttctgttag cgttggcgca ggatatgatc
     4141 ctcgccgcca ttcctgcggt cggctttgcg atggtgttca acgttcccgt tcgggcgtta
     4201 cgctggtgtg cgctgcttgg cgcgattggt catggttcac gaatgatctt aatgaccagc
     4261 gggctgaata ttgagtggtc aacttttatg gcttctatgc tggtcggcac cattggtatt
     4321 caatggtcgc gctggtatct ggcgcatccg aaagtgttta ccgtggcggc cgttatccct
     4381 atgttcccgg gcatatcggc ttataccgca atgatttcgg cggtaaaaat cagccagtta
     4441 ggttacagcg aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt
     4501 gttggtgcgt tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct
     4561 cgcgtataaa attgcctcgc ttaagcacac ggatgagaga cagcctcctc tcctccgtgt
     4621 gttactataa aagttatctc ccttctcgtt catcgttcca tatttgagaa acagtatgtc
     4681 ttccagagtt ttgaccccgg acgtcgttgg tattgacgcc ctggtacacg atcaccaaac
     4741 cgttctggca aaagctgaag gcggtgtggt tgccgtattt gctaacaatg ccccggcgtt
     4801 ttatgccgtc acgcctgcac gcctggctga actgctggcg ctggaagaaa agctggcgcg
     4861 tccgggaagc gatgtcgctc tggacgatca actctatcag gaaccgcaag ccgctcccgt
     4921 tgctgtcccc atggggaaat tcgccatgta tccggactgg caacccgatg ccgactttat
     4981 ccgcctggcg gcgctatggg gcgtggcgct aagagagccg gtgaccaccg aagaactggc
     5041 ctcattcatt gcctactggc aggcggaagg taaagtcttt caccatgtgc agtggcaaca
     5101 aaaactggcg cgcagcctgc aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga
     5161 tgtgaatacg gtcagcgaac ctgacagcca aattccacca ggattcagag ggtaacgatg
     5221 aaaaacgttg gcgacctgat gcaacgcctg caaaaaatga tgcctgccca tatcaaacct
     5281 gcattcaaaa cgggtgaaga gctactggcc tggcagaaag aacaaggggc gatccgctcc
     5341 gccgctctcg aacgtgaaaa tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt
     5401 attcgtccac tgcatcagaa ctgctccttt gagaactatc gcgttgagtg tgaagggcag
     5461 atgaatgcgt taagcaaagc gcgccagtat gtcgaagagt ttgacggcaa catcgccagc
     5521 ttcatctttt ctggtaagcc aggaaccggc aaaaaccatc tggcggcggc aatctgcaac
     5581 gagctgctgc tacgcggtaa atccgtattg atcatcaccg tggccgatat tatgtcggcg
     5641 atgaaagata ccttcaggaa tagcggtacc agcgaagaac aactgcttaa cgatctgagc
     5701 aacgttgatc tgctggtgat cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa
     5761 gtgatcatca accagatcgt cgatcgccgc tcttcttcca aacgcccaac cgggatgctg
     5821 accaacagta atatggaaga gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg
     5881 cgcctgggta acagtttgtg ggtgatcttc aactgggata gctaccgtag ccgggtaaca
     5941 ggtaaagagt attaatcccc gtttctggcg gtatactgcg acacaattcc accttgagta
     6001 atgatgatga aaactgttaa acatcttctg tgctgtgcca ttgccgccag cgcattaatt
     6061 tccaccgggg tgcatgctgc gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt
     6121 ggcaaccaaa acagcacgac acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg
     6181 cttagcagcg ggaaccaggc cttaagcgca gataacatga acaacgccgc aggcattctg
     6241 caatactgcg cgaagcaaaa gctggcgtcg gtaaccgatg ccgaaaacat caagaaccag
     6301 gtgctggaaa agctgggcct gaacagtgaa gaacaaaaag aagacaccaa ctatctggat
     6361 ggcattcagg gtttgctgaa aaccaaagat ggtcagcaac tcaatctgga taacatcgga
     6421 acgactccgc tggcagaaaa ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg
     6481 ctgaacttca tttcctgaac cgcgaaacgc cgcgttttag ctgttacccg gctgacgcgg
     6541 ctctctcctg cccttttccc tgctcctgga cggttttccc cctgttacac gggaagcgac
     6601 cgcgatcaaa aaagtctgtt tataaactaa ttcctaacaa catcgcattt ttttgacact
     6661 agaattgcag caatatggcg acgccattac attgtgcttc tcaaaaagct taaacgttgg
     6721 ctaaacggcc cggtttagtg aggatcgccc gttgtcagaa ctactctctt tcgccctttt
     6781 tctcgcctct gtgctgattt acgcatggaa agcgggacgt aacacctggt ggtttgcagc
     6841 cacattaacg gtgctggggc tatttgtcgt tttaaatatc accctgtttg ccagcgacta
     6901 ttttactggc gctggtatta acgacgcggt tctctatacc ttaaccaaca gcctgaccgg
     6961 tgctggcgtc agcaaataca tcctgccggg tatcggcatt gtgctggggc tgacagcggt
     7021 gttcggtgcg ctgggctgga tcctgcgccg tcgtcgccat catccgcacc attttggtta
     7081 cagcctgctg gcgctcttac tggcgctggg ttcggtggac gccagcccgg catttcgtca
     7141 gataacggaa ctggtgaaat cccagtcacg cgatggcgac ccggactttg cggcttatta
     7201 taaagagccg tcgaaaacta tccctgaccc gaaactcaac ctggtttata tctacggcga
     7261 aagtctcgag cggacctatt ttgataacga agctttcccg gatctcacgc ctgaactggg
     7321 cgcgttgaaa aatgaaggcc tggatttcag ccacacgcag cagctgccgg gaacagatta
     7381 cacgattgcg ggcatggtgg cttctcagtg cggcattccg ctttttgccc catttgaagg
     7441 taacgcctcc gcttctgttt ccagcttctt cccgcagaac atctgtctgg gcgatatcct
     7501 gaaaaactcg ggttatcaga actatttcgt gcagggcgcg aatctgcgtt ttgccggtaa
     7561 agatgtgttc ctgaagtcgc atggcttcga ccacttatac ggctcagaag agctgaaaag
     7621 cgtggtagcc gacccgcact atcgcaacga ctggggattc tacgacgata ccgttctcga
     7681 tgaagcgtgg aaaaagtttg aagagctttc ccgctcaggt cagcgattct cactgtttac
     7741 cctgacagtc gatacccatc acccggatgg ttttatctcg cgtacctgta accgcaaaaa
     7801 atatgatttt gacggtaagc cgaaccagtc attcagcgcg gtaagttgca gccaggagaa
     7861 tatcgcggcg tttatcaaca aaatcaaagc gtcaccgtgg tttaaagata ctgtcatcgt
     7921 cgtctcttct gaccatttag cgatgaacaa cacggcgtgg aaatacctca ataagcagga
     7981 tcgcaataac ctgttttttg tcattcgtgg cgacaagccg cagcaagaga cgctggcagt
     8041 gaagcgtaac acgatggata acggcgcgac ggtgctggac attctcggtg gcgataacta
     8101 tctcggactt ggtcgtagca gtttatccgg gcagtcaatg tcggaaatct tcctcaatat
     8161 caaagagaaa acattggcgt ggaagccgga tatcatccgc ctgtggaaat tccctaaaga
     8221 gatgaaagag ttcaccatcg accagcagaa aaacatgatt gccttctcgg gtagccattt
     8281 ccgtttgccg ctgctgttgc gggtttcaga caaacgcgtg gaaccgctgc cggaaagcga
     8341 atactcagca ccgctgcgtt tccagctggc cgatttcgct ccacgcgaca atttcgtctg
     8401 ggttgaccgt tgctacaaga tggcacaact ctgggctccg gaactggcac tttccaccga
     8461 ctggtgtgtc tcgcaggggc agcttggcgg tcagcaaatt gttcagcatg ttgacaaaac
     8521 aacatggcag ggcaaaacgg catttaaaga tacggtcatc gacatggcgc gttacaaagg
     8581 caatgtcgat acgctgaaga ttgttgataa cgatattcgc tacaaagccg acagtttcat
     8641 cttcaacgtc gccggtgcgc cagaagaggt gaaacagttt agtgggattt cccgtccaga
     8701 gtcgtggggc cgctggtcca acgcgcagct gggcgatgaa gtaaaaatcg agtacaagca
     8761 tccgctgccg aagaaatttg acctggtgat taccgccaaa gcatacggca ataacgccag
     8821 ccgtcctatt ccggtacgcg taggcaatga agaacaaacc cttgtgctgg gcaatgaagt
     8881 gaccaccacc acactgcatt tcgataaccc aaccgatgcc gacacactgg taattgtgcc
     8941 gccggaacct gtctcaacca acgaagggaa tatcctcgga cactcgccgc gtaagctcgg
     9001 gatcggcatg gtggaaatta aagttgtgga acgtgaaggg taattcctga ctgatgtgta
     9061 ggtcgcataa ggtgcgacct acacaccttc tccggcctac aaaacatcat tgagaaacag
     9121 aaaaaaacag cgcccaacac ttccccggac gctgcatggc aactttaata atctcgcctt
     9181 atcagaaagt aattacacct ttaattaact cacgattgtt aatcacatcg cgctcgtagg
     9241 tttctgccag cgtggcgaac ggatagcgat gggttaacat catgtcagca gtgatttttc
     9301 cttccgccat cagtcgaccg actttagcaa aatcttccgg cgtggcgttg cggctgccca
     9361 tcatcgtcgt ttctttttta tggaattccg gatcggagaa ctgcaactca cctttaaaca
     9421 ggccgacaaa taccaccgtg ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat
     9481 gttgattacc tgtcgcgtcg atcactttct gcgccagcga accaccaaac tgcgcccgca
     9541 actgcgcgtc aaaatcttcg gctgacgggt ccagcacggg caactccaga cgcgttgcca
     9601 catgttcacg gcgcgccgga ctggtatccg ccaccaccac ctgtgcgcca tcggctttag
     9661 cgattgccgc cgcgcccaga ccgattggcc ccgccccgac caccagcacc tgctcgccgg
     9721 gagcaatggc tgcgcgacgc accgcatgag cgctaatagc gaaaggttca atcaatgcgg
     9781 ccgcctgcgg gtcaataccg tctgcgggca aaatgttcgc caccggcacg ctcaaatact
     9841 cactaaaacc gccatcctga tgcacgccaa tgactgaaat tttttcacag caattggtac
     9901 gcccgctttt acacgccggg cattgctgac aagaaatata cggaattacc gcaacttgct
     9961 gtccgatatt aaaattgtga aagttttttc ctgattcaat aatctcccca catatttcat
    10021 ggcctaaaac gcgtggatag ctaaaaaatg gttgattgcc cgcccaggca tgaatatctg
    10081 taccgcaaat cccgacggtt ttaattttaa ttaatgcttc attatcgcct ggcaccggtc
    10141 tttcccggac tgtccatact aaattcttta tttcctgaca aattaatact ttcattgtgg
    10201 acatgattca ccgctccttt tattttatga ctttcagata tcgtcgaatt tataagagca
    10261 cctgaattta cccataaaat atgcggatga tcacgcataa aaatgtttta aatcggtttt
    10321 taatgccgga ctggagggaa gaatgagtcg ttcgcagaat ttacgtcata acgtcataaa
    10381 tcaggtgatt gaagatatgg cgaggggcaa tattccttct ccgctgcctt cacaaagtgg
    10441 gctggcagaa atgtataaca tcagtcgcac tacggtacgc cacatactcc agcatttaag
    10501 cgcatgtggc gtgctcaccc tggtggggaa aaattatgtt atcgcccgca aacccgaaca
    10561 gaacgatgga tttgattgca tcacggcctc gctgaaagag caaaaccgtc ttttcgagca
    10621 ggcatttttc accatgatta acctgcgcca gttacgcgcg ggagaaagct tttccgaact
    10681 tcagctggcg cgagcggcag gcgtcagtcc ggtagtagta cgagaatatc ttttgaaatt
    10741 cggtcgttat aatcttattc aaagcgaaaa gcgcggccag tggagcatga agcagttcga
    10801 tcaatcctac gccgagcagc tgtttgagct acgcgaaatg ctcgaaacac attccctgca
    10861 acactttctc aaccttcccg atgacgatcc ccgctggttg caggcgaaaa ccatgctcga
    10921 acgtcacagg acattgcgcg acagcattgg cagcaacttt cggatgtttt ctcaattaga
    10981 ccgtgatttt catgccatgc tgttatccgc tgctgacaac atctttttta atcaatcact
    11041 tgaaattatc tcggtgatct ttcattttca ctatcaatgg gatgaaagcg atctcaagca
    11101 gcgcaatatc attgctgttg atgagcatat gaccattctc agcgccctga tttgccgtag
    11161 cgatttagat gcaataacgg cattgcgaaa ccatctggat accgccaaac aatcaatgat
    11221 tcgttccatc aggcaacatc accgataaat taagtacaga ttaaaaacat tgattcttgg
    11281 tttatatcac tatgttgtat taatgcagta ttaatccagg cgattgttct gcccataagg
    11341 aaatacatag ggaataaacg atgctggaag tattactctc agccatcatt taactcccta
    11401 ttactggtag cgataaaatt atcggctatc catgattaac gtttccagca catctaattc
    11461 actgactatt tttgccagtg tttcatcact gacttcttgc cgtgcacgca tctggtaaat
    11521 agccgcacgc ctggcattta acgctgccag attaaatgtt ctttccagct tcgcgcgctg
    11581 catatgtaaa gaattatcat tttccgacat atcatcttcg ctacgccgca tataaccgat
    11641 aactctggcc cctgtttcca taatcaactc gttgtcgagg ttttctgtac tggtgcgtag
    11701 caaacgttgc tcagtctttt gcacactgag tatggccatt ttggccataa acagatcggc
    11761 agcttcctct tctttttgcc ggttatcgct gaaaatgccc acatttttcc ccagtagtaa
    11821 cggcagaata ataatgccgc aaaccagcga aaagaggatc acacctgtgg cgagaaatac
    11881 cagtaagtaa cgcccgggaa aaggtgtgcc atccggcagc aataacggga tagaaagcac
    11941 ccccgcaagc gtgatggctc cgcgcacccc cgcaaaggtc gccagcagca tatgacgcgt
    12001 tttatatcca gcaaacagca tcggatgttt gccgggtcgt ttacggctat acagacgcat
    12061 cagccaaagc cagcccaggc gcacaaccat taacgccagg tagatccaaa tgacatcaag
    12121 gagcagcgat ccggtgtcta ccagtggatc gttttctgca cggatcagtg attccgaaat
    12181 aatgcccggc agttgtaagc ctaaaatcag aaacaccatg ccgttaaaga catattcaag
    12241 catcgaccag ctacttttag ctttaattct caggcggata ggcgcgtggt ggactacgcc
    12301 aatgcgactg gttgttaacc ccgcggcgac agcggcaaga atgccagata agccaaaatg
    12361 ttccgcaacc agataaacaa taaacggcaa cagaagcatc aatacaatct gaatggaagg
    12421 ttcatcgtga gcatatcgac tgatcaacag cagccctttg ccatataacc aggtaacagc
    12481 aatccccacg gcaataccgc ccaacgcaac gacaaaaaac gcaatggttg cgccatgaac
    12541 gctgaattcc atcgttccca tcgtcactgc aacagcaaat ttcaacgcca ccaggccgga
    12601 agcatcattc atcaacgctt cgccttcgag cacttcctgg atgtttttgc tgatacgccc
    12661 tttcccgaca atccccagca acgccacggc atcggtaggc gacaacacgg cggctaatgc
    12721 gaaagcagcg atgagcggca cattcggtaa gagtaaatga atcacatagc cgacaccaaa
    12781 aatagtgatt agcaccaaca caagcgccag gctgataatt tcccgactgt gcttgatcaa
    12841 ttctttaggt tgtatttttg agctttcagc aaacaacaag gggggtataa acaataagag
    12901 aaaaagttca ggattgaatt ccacatgcag accaaatatg ggctgggcca gtacagcgcc
    12961 agcggcaatc tggatcaaag gaagtggtat ctgaacagga gacagacgcg taagcacgct
    13021 ggttaatgat actaccaacg taagaattaa aattgttaaa agtatttcca tataataaac
    13081 aggcttcctc attcataaat aatgggtatt attaatttaa cttcataaaa agcgccctgg
    13141 ttagcactta acgttaatca ttaatcacag cgttcactca catgctcagg aggaaaccag
    13201 tgagatgagt tttccagttg ataacaaatt caccatgtga atatttacta cctttaaccg
    13261 ttgtaacgaa tcatccgtta ctctttgatc tccttgccga acaagttatt tagtgaatga
    13321 tggctttctt ttaacgttag catgaattat acgttcagca aatgtattaa atcagcacta
    13381 aataaattta atggatacca ccattctgcg tcaacactta gtttttatct gtaaaaatag
    13441 ccgcagccaa attaatgctt ttattttaat aaatcatcaa ccgaataaga gatcttatca
    13501 gcggtaacta aactcatcag tgggtgttcc ttgtaacgtc atacattaca taactttaag
    13561 atatacaatc aaaaaatgca ttaaaaaccg attaaaagca gcaaagtatt acccggtgca
    13621 cgaatttatg acacatttgc agccattccg ctattcgctg actacttttc agtaatgact
    13681 gctttttcat aaggtttcac tcccgacaac aatgttggag ttttatttcc ttagcatgga
    13741 gtaagtaaag atgagcaaaa taatagcgat gctaattact gtcagtaccc tttttttacc
    13801 gtcaataatc caggcaaaac ctatttcgat aaaagttgcc tatgaaaata accctggaga
    13861 acccctggat gtggtcatgc gttactgggc agacctatta aataaaaaaa gtaatggcga
    13921 aataacactt gcgctctatc caagttccca attgggctca aaacaggatg tcaccgagca
    13981 agccatgatg ggcatgaatg tcataacctt gagtgatgtg gcctttcttg ccgattatga
    14041 acccgatttg ggtattttgt ttggaccgta tctgactgac gatccgcaaa aactgttcaa
    14101 aatatatgaa agcgactggt ttaaacaaaa aaatgaaagt ctgaagaaaa agggaattca
    14161 tgtggtaatg aataattatc tctatggcac tcgacagatt atttcgaaaa aacccattcg
    14221 cactgttgac gatctcgcag gcttaaaaat tcgtgttcct aataacgtca tgcaaataaa
    14281 agccattcag gcgatggggg caacaccaac tcccatgcca ttaggcgaag tttatcccgc
    14341 attaacccag ggcgttattg acggcgtaga aaacccaatt tcagtgctgc aagggcaaaa
    14401 actgtatgag caggcaagat atctgacaat ggttaactac ctgacaaata cctcagtatg
    14461 gataggaggt gaagccttct ttagcaccct ttcaccagaa caactggaga tgatccacca
    14521 gaccggctat gaagcgggtg tctatagcca gaaactcact cttgaacgtg atgccgaaat
    14581 gttgaaaacc atgcaggcag cgggcgtcga agtgatctat cccgatactg gaccattcca
    14641 gaagaaagca cgcgaagttt actctcaatt cccggaatgg acaccaggtc tgtacgaaac
    14701 cattcaacag caactgcaat aagcattaat gctcagcgtc ctgcctcatc tctcttaggc
    14761 aggacacgca ctaacaggag gtttcatgcg tctttttggc aaagtggcag aatttagtgc
    14821 tgcctttgcg ctattcgttc tggttgtgat gacaattgga gcggtattta tgcgttattt
    14881 catcggccaa ccgcttcaat ggacagaaga aatgtcggga atgctgatga tttgggtggt
    14941 gatgttaggt ggagtggtgg cggaacgtga cagggcgcac ctgaccattc ccttcctgat
    15001 ggagatgctt ccaggcaagc tcaggcgtgt tattgcagta ctggtcgccg ttctctccat
    15061 cgcactgctg ctgtatatgg cctggctggg ctaccgacta gccgaaatgg cgcagtttaa
    15121 agtgacacaa cttctgaagg tttcctggtt ctggatcgat ctggcggtcc ctgtcggtgc
    15181 actggcaacg gctatttata ccctctactg gttaatacac gatataaaac aatctgatag
    15241 cggagacaaa atgcaatgag ttggttattg attattccgc taatgatcgt ctgtttgttt
    15301 ctcaacattc gggtctatct ggcgatgttt attggcatca tcgcttactt tttattcttc
    15361 tccagcgtac cgattgaaat tgccgtgcaa agactgatcg cgccaacgca aagcccatca
    15421 ttgctggcaa ttccgttttt tatcttgctc ggcacactaa tgagctacac cggcattgcc
    15481 gaacgcatcc tgcacattgc caacgttctg gtggggaaaa tgcgcggcgg acttggcgtg
    15541 gcaaacattc tcgtcagcac catgatgggc ggcgtcagcg cctctaacct tgctgacgcg
    15601 gcgatgctgt cgcgcatgat ggtgccggag atggagcgta aaggctacaa ccgcgcgttt
    15661 gccgccgcca ttaccgctgg tggctcgtta gtcaccccga tcataccgcc gggaatagcc
    15721 ctgattattt acggtctggt tgccgacgtc tctatcggaa aaatgtttat cgctgggctg
    15781 gtacctggtt tgctatgcgc agtcgttctg atgtttaccg tctacctggt cgcccgcaaa
    15841 actaacgcta aaccctcgcg tgaaagctgg cctacgggca aagaggtggt gttctcgctg
    15901 ggacaggcct ggcctgcgct gttcctcatt tttgctgtgg tgggtggtat tcgcgctaac
    15961 attttcaccc caacagaagc aggtgcagca gcggtgctaa tcgtgctggc gattggcttt
    16021 tttatctatc gtgaaatgcg gatttctcat gtcgtcaaag cattgggcga aacggcacgc
    16081 gcaaccgcat cggtgatgct ggtaatcatg gccagcgccg cgttgggatg gattttctca
    16141 atggaacatg ctggtgtcgc agtggcgaat tttgtgactt cgctgaccga aaataaatat
    16201 atgttcctgt taattatcaa catattgctg ctgacgctgg ggatgtttct tgaaggaaac
    16261 gccattttgc tgatcctggt accactatta aaaccggtgg tcgaacactt tggcatcgat
    16321 ccggtgcatt ttggcatcat catgatcttc aacctgtcga ttggcgcatt cacgccaccg
    16381 gtaggtaccg tgatgctgct ggtatgtaac atcacgcaag tttcggtggg aaatttcttt
    16441 aaacaatccc tgcctctact ggcggctcta ctattaatgc taacgctggt gacatatatc
    16501 ccggcgattt cactgttcct ggtttaataa taaaaacgcc ggatgaaata ctcatccggc
    16561 gtcattgcgt taaaacgttt cccagttctc cccgctatct gccacggcca ttttacgcgg
    16621 cgcagctggc gtcacggttt ttaccacagc cgatgtttca cgctgctgtt gctgctgttg
    16681 ctgaatccgg aacactgcca cagcttcggt caggcgactg gcctgctctt ccagcgcggc
    16741 ggcggcggcg gcagattctt ccaccagcgc ggcgttctgt tgagttaccc ggtccatctc
    16801 agcaaccgct aagccaacct gatcgatacc acggctctgc tcatcagaag cagaagcaat
    16861 ttcgcccata atgtccgtca cgcgggtcac agcactgaca atctccgcca ttgtttcccc
    16921 ggcgctttcg accagcgtag agccaacatc cacttttccc accgagtctt caatcaggct
    16981 tttaatttca cgagccgcct gagcgctacg ctgggccaga ttacgcactt ctcccgcgac
    17041 caccgcaaaa ccgcgtcctt gctcacccgc acgcgcagcc tcaaccgccg cgttcaaagc
    17101 cagaatattg gtctggaagg caatgccgtc aattacgctg ataatatcgg cgattttctg
    17161 cgaactggtg gagatatcgc gcatagtctg caccacgtta tctaccactt tgccgccgcg
    17221 ttgcgccgtt tcagaagcac ttaatgccag atggctggcc tggcgcgcat tctcggcgtt
    17281 ctgtttcacc gttgcggtca gttgctccat gctggctgcc gtctcttcca gcgaagcggc
    17341 ctgttgctcg gtgcgcgaag agagatcgtt attgccggta gcgatttcgc tggcaccgct
    17401 atagatggca ttggccccgt tgcgcacatc accgacggta cgcatcagct ctccctgcat
    17461 atggcgcaaa ctctctgcca gttgccccat ctcattagag ccatccacct caatcggttt
    17521 caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc aggcgattca ttggcgctac
    17581 cagcgaggct ttaataccga accagacggc gaagatgacc gccagtacga cgatcatcac
    17641 gcccaccaga atccacatcg cctggctgta ggaggcattg ttatcgctga cggcgatatc
    17701 atagagccga tcgttttgct ccatgtaagc cacatactgc ttctcgaaac cgtcctgata
    17761 tccctgggtc ggctgatcaa agaactcgtt gattttgcct gcacctaata gttggatcag
    17821 ctccgccagc gcattgtgat aaatatcgta attacgtttg atctctgccg ctgcggcggt
    17881 gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc cagttttttt ccgcctgttt
    17941 cagcgaaata ctggcactct gcatcagctc agcaacggtt gaaccgctac caatattatt
    18001 ctgatccatc atgtagcgga tacccgcgcg gttgagggtg ttacgcgtct gcaacaacgc
    18061 gacccagctg ccattcagcg tggattgctg ctggcgaatg gtttgtaaaa cagtgaaatt
    18121 ttctttgtca ttctttaagg cattaaagaa cagaccgcct gatgtcagtt gtaaaaggcc
    18181 aaaaacggcc aaaaccagca gtaagctggt cacaattttg atacgtttta acatggtttc
    18241 tctttcctgt ggaccgatgc aagattttcg gcctggaaag caaaaacttt atgaaaaggt
    18301 gtaacccgcc atccatggaa aaaccaggcg ctggttatca gatactaaaa agttattcat
    18361 tatcgcccgg gatgtcttct tgtcgggaat tacccagagc aataaattcc tctaacagat
    18421 gggttaactg ttgcattttc tcggcagtaa actgagcttc aatctgccga taggcttctt
    18481 caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc tttggtcagc gagatataga
    18541 gcttccgctg atcattgatc ggctttaatc gcaacactaa accgtcgcgt tccatccgcg
    18601 tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg atacgccaga tcgtgaaaat
    18661 ccattgatgg gctttccgcc agaatacgca cgatgcgcca ctgttgctca gtcaaattat
    18721 gccgtttaac gattgggcga aaataactca tcgccgcttc tcgcgcctgg agcaacgcaa
    18781 tggttagaga gtcgtgcatt atctttcccc tgaaaaattc aaaatcatta atatagaaac
    18841 agttaatcat cacgttaccg ggactgcgct gcccctgcaa ttcttcattt tatcaaaatt
    18901 gagcatgt
//
LOCUS       DAZFKM010000096        18650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFKM010000096 DAZFKM010000000
VERSION     DAZFKM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..927
                     /locus_tag="ACHKAY_003445"
     CDS             <1..927
                     /locus_tag="ACHKAY_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO5702060.1"
                     /translation="LPLIIFYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHPRSADN
                     IDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSA
                     SSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEM
                     GSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQD
                     INAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNK
                     QYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            1030..1533
                     /locus_tag="ACHKAY_003446"
     CDS             1030..1533
                     /locus_tag="ACHKAY_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO5702061.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1600..3057)
                     /gene="nuoN"
                     /locus_tag="ACHKAY_003447"
     CDS             complement(1600..3057)
                     /gene="nuoN"
                     /locus_tag="ACHKAY_003447"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO5702062.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3064..4593)
                     /gene="nuoM"
                     /locus_tag="ACHKAY_003448"
     CDS             complement(3064..4593)
                     /gene="nuoM"
                     /locus_tag="ACHKAY_003448"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO5702063.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4757..6598)
                     /gene="nuoL"
                     /locus_tag="ACHKAY_003449"
     CDS             complement(4757..6598)
                     /gene="nuoL"
                     /locus_tag="ACHKAY_003449"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO5702064.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6595..6897)
                     /gene="nuoK"
                     /locus_tag="ACHKAY_003450"
     CDS             complement(6595..6897)
                     /gene="nuoK"
                     /locus_tag="ACHKAY_003450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO5702065.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6894..7448)
                     /gene="nuoJ"
                     /locus_tag="ACHKAY_003451"
     CDS             complement(6894..7448)
                     /gene="nuoJ"
                     /locus_tag="ACHKAY_003451"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO5702066.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7460..8002)
                     /gene="nuoI"
                     /locus_tag="ACHKAY_003452"
     CDS             complement(7460..8002)
                     /gene="nuoI"
                     /locus_tag="ACHKAY_003452"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO5702067.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8017..8994)
                     /gene="nuoH"
                     /locus_tag="ACHKAY_003453"
     CDS             complement(8017..8994)
                     /gene="nuoH"
                     /locus_tag="ACHKAY_003453"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO5702068.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8991..11717)
                     /gene="nuoG"
                     /locus_tag="ACHKAY_003454"
     CDS             complement(8991..11717)
                     /gene="nuoG"
                     /locus_tag="ACHKAY_003454"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO5702069.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11770..13107)
                     /gene="nuoF"
                     /locus_tag="ACHKAY_003455"
     CDS             complement(11770..13107)
                     /gene="nuoF"
                     /locus_tag="ACHKAY_003455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO5702070.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13104..13604)
                     /gene="nuoE"
                     /locus_tag="ACHKAY_003456"
     CDS             complement(13104..13604)
                     /gene="nuoE"
                     /locus_tag="ACHKAY_003456"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO5702071.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13607..15409)
                     /gene="nuoC"
                     /locus_tag="ACHKAY_003457"
     CDS             complement(13607..15409)
                     /gene="nuoC"
                     /locus_tag="ACHKAY_003457"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO5702072.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15503..16165)
                     /gene="nuoB"
                     /locus_tag="ACHKAY_003458"
     CDS             complement(15503..16165)
                     /gene="nuoB"
                     /locus_tag="ACHKAY_003458"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO5702073.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16181..16624)
                     /gene="nuoA"
                     /locus_tag="ACHKAY_003459"
     CDS             complement(16181..16624)
                     /gene="nuoA"
                     /locus_tag="ACHKAY_003459"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO5702074.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17255..18193)
                     /gene="lrhA"
                     /locus_tag="ACHKAY_003460"
     CDS             complement(17255..18193)
                     /gene="lrhA"
                     /locus_tag="ACHKAY_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO5702075.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4662 a         5170 c         4811 g         4007 t
ORIGIN      
        1 ctccctctta ttatttttta ccaaccctgg gtcaatgcat taccgccaat gccccgacac
       61 gcaaatcctg agcaattaga aaaaacagtg cgttatctta cacaaactgt gcatccacgt
      121 agtgccgaca atattgataa tctgaatagg tccgcagagt atataaaaga ggtcttcgtc
      181 agtagcggtg ccagaattac ctcgcaggac gtccccatta cgggtggccc ctacaaaaac
      241 attgttgctg attatggtcc tgccgatgga ccgctgatta ttattggtgc gcactatgac
      301 tctgccagca gttatgaaaa cgatgaattg acctatacac ctggcgcgga tgataacgcc
      361 agcggcgtgg caggattact cgaactggca cgtttgttac atcagcaagt accgaaaaca
      421 ggcgtgcagc tggtcgccta tgcgtcggaa gaaccgccct tctttcgtag cgatgaaatg
      481 gggagcgcgg tacatgcagc ttcgcttgag cgtccagtaa aattaatgat agcactggag
      541 atgattggct attacgactc tgcgcctggt agccaggatt acccttaccc ggcaatgtcc
      601 tggctttatc ccgatcgggg agactttatt gccgtggtcg gcagaatgca ggatatcaac
      661 gccgttcgtc aggtaaaagc gacattgtta tcatcccggt atttatctgt ttattctatg
      721 aatgccccag ggtttattcc cggtattgat ttctctgatc accttaatta ttggcaacac
      781 gatattcccg ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttg
      841 cccggtgata ccgcagacag attgaattat cagaaaatgg ctcaggtggt ggatggtgtt
      901 ataaatttgt tatacaacag taaataataa aaatttctcg aggcgggtcc agaaaaagcg
      961 acatgcacat tttattttcc ctcgtctgtt ttaagatgcg aaacatgaaa ttcaatggag
     1021 gaaaaataaa tgggaatgat tggttatttt gcagaaattg actcagaaaa gattaaccaa
     1081 ctgcttgagt ctactgaaaa acctttgatg gataatatac atgacacgct ttcaggatta
     1141 cgccgactgg atattgataa acgctgggat tttttacact ttgggctgac aggcacctct
     1201 gcttttgatc ccgcgaagaa tgatccgctg agtcgtgccg tgcttggtga acacagcctc
     1261 gaagatggca tagatggctt cctgggattg acctggaatc aggagttagc cgcgacaatc
     1321 gaccgactgg aatcactgga tcgaagtgag ctgcgtaaac agttttccat taagcgactc
     1381 aatgaaatgg agatttatcc gggagtgacc ttttcagaag agttagaggg gcaactcttt
     1441 gcttcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat gctgcgacaa
     1501 gggaatcatg cgctgacggt tattgttggg tgatggtgtc agggcaccaa aaaaaaccgc
     1561 cgaacatgtc ggcggctttc tgacttacaa actaacagat tacatcagcg gcattgctaa
     1621 ccgcacaatg ctaatcagcg gttgtggcca tacacccagc accagtacca acagtgcaga
     1681 aatcagcacc acgataccgc ccgcgctgta ctgccagttt gatggtgcat cgcgacccgg
     1741 ttgttccggg gcgtgaagat acaggctcac cgccacgcgc aggtagtagt agaggccgat
     1801 tgccgaaccg acaaccacgg cacccaccag ccaccacaag tgtgcctgga caccgaccgc
     1861 cagcacgtag aacttaccga taaagcccag cgtcatcggg ataccggcca gagacagcat
     1921 catcaccgtc atcactgccg cgaggatcgg acgatgccag aacagaccgc ggtaagagaa
     1981 cagggaatca gcatccgggc cacgatacgg gctggacatc aggctgacca cgccgaacgc
     2041 gccgaggctg ctgaacagat aaccggccag gtaaacccct accgcttcca tcgacatctc
     2101 accagtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga tagatgagta
     2161 accgagcaga cgtttgatgt tggtctggct cagcgccatc aggttaccga agatgatgga
     2221 ggcaaaggcg ataatcgcca gtactacgcg aatcgcttcg ctgtcaccca ccggtgcgta
     2281 gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg ccaggaaagt
     2341 ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga acggcaccag
     2401 cgagagtttg aagccgaggc caacaatcat cagtccgaaa cctgccagca acagcggctc
     2461 gttgagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt cgccagactg
     2521 tgcatacacc agcgccatac caaacagcag gaaagaagac gctgcggcag aaaggatggt
     2581 gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa
     2641 cagcggcaaa gagatcagtt caataccgag gaacaaagac gccagatggt tggcattcgc
     2701 cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcat ccttgttgtc
     2761 gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac
     2821 cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc
     2881 gcccgcctgg ccgacaaacc agagcgaaac cagcgccgcg ttaagcccaa taaccgagag
     2941 cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt
     3001 caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc
     3061 gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat tgccaatcgc
     3121 ggagcgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca gcagcaccac
     3181 cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct ggctggcaat
     3241 ctggcttttc gctttaccga agtaagcgcg atgcaacatc gccagcgaat aaacagatgc
     3301 aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga agctgccgaa
     3361 cagaatcata aattcgccga cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc
     3421 aaagaacagc gacagtgctg gcagccattt catcttgctc cacagaccgc ccatcatgcg
     3481 catgtcgcgg gtatggatac gttcataaag ctgaccacaa agaataaaca gacccgccgc
     3541 cgacaagccg tgcgcaatca tctggattac cgcgccctgg taggccaact ggctgccggt
     3601 gtagatagca atcagcacga agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt
     3661 gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa cacccagcca
     3721 catagcaatt ggcgcgaact ctgccgacgc gttcgggaac agcggcaggg agaaacgcag
     3781 caaaccgtaa gcggcagttt tcagcaagat ccccgcgagg tcaacggaac cggcggtcgg
     3841 agcctgggag tgcgcatccg gcagccagcc atgcagcgga accaccggca ttttgactgc
     3901 gaaggcgatg aagaagccca gcattaacag gtattccaca ccactggaca ttggcgtatt
     3961 cagcagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa caaaaaccag
     4021 cgccaggatg gcaatcaaca tcaccaggcc actcgcctgg gtgtaaatga agaacttggt
     4081 tgccgccgtg atacgcgttt taccgtcaga ggctttatgc ccccacagtg cgatcaggaa
     4141 gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt cgatggcaag
     4201 gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga agccctgata
     4261 tttttcgatc tctttccacg aacatagtac cgccagcaca ccgagcagac cggtcagcac
     4321 gaccatcagc agcgacagcc cgtcaatggc gagatgaata gagataccaa aacgcgggat
     4381 ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg tcagtgaata
     4441 accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc
     4501 gatccagcgt ggcaccttga cgccaaagcg ttcggtctgc cagcacagga agccgccaat
     4561 aaagggaatt aatattagcc agggtagtaa catggcgatc tttattcctt gtaaaagtcc
     4621 cgtcaggacc ggattttcaa cgaattctca cgacagaaaa ttctcttcgg actgggggta
     4681 gtgtcggatg caccgcgaag gcggcttatc cgacctacgt tcttatatgc ccaggggcaa
     4741 tcccacaatc cttaactcaa cgcagtacca tcaacagtgc cagcaccacg accgcaccga
     4801 tgctcatgga tgccacatac cagcgcagat agccgttctc acttaacagc agacctttac
     4861 ctgcaaagcg ggaaaggaca gccgggatgt tcatcattga gttcagcgga tcgcgtttca
     4921 gcaaccaggc aatacccagg aacggcttga cgaacacttt gtcatacagc cagtcaaatc
     4981 cccaggcgtt gtaccaccag gtgcccagca gacggcccgg cgcactgttg gcgatggagg
     5041 tcaccagagt acgtttaccc agccacagcc aggctgccag cagaatgccg accaccgcga
     5101 ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg
     5161 gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa aggatcagca
     5221 gcacaatcag cggcaggctg tgagttaccc ctttcacggc gtgagcgtga atttgttctt
     5281 ttccgtggaa gacgatgaaa atcatacgga aggtgtagag cgaggtcata aacgcaccga
     5341 ccagacctgc caccatcaga ttgatatgac cattcgccat cgcacccgcg aggatctcat
     5401 ccttactgaa gaagcccgca gtgaccagcg gtagtgccga cagtgctgcg ccgcccacca
     5461 ggaagcagag ataaaccagc ggaatagatt tacgcagacc gcccatcttg aagatgttct
     5521 gttcgtgatg gcaggccaga atgacggaac cggatgccag gaacagcagc gctttaaaga
     5581 acgcgtgagt catcaagtgg aaaatcgccg catcccatgc ctgcacgcca agcgcgagga
     5641 acatgtagcc aatctggctc atggtagagt aagcgagaac acgtttgatg tcggtctgta
     5701 ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc gacaataccc accagatgca
     5761 gaacttccgg cgtcaccagg aacaggccgt gggtacgggc gatcaggtag acacccgcgg
     5821 ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcag
     5881 caagccatgt ctgcaacggc aactgcgcag atttaccgac cgcaccgccc agcagcatca
     5941 gcgtcgccca catcagcatg ttattgccgt cagcaaagtg cgctggtgcc agttccacca
     6001 tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat gaacagtgcg aaagcgagga
     6061 aaacgtcacc cacacgggtc acgacgaacg ctttcattgc cgctgcgcca ttcttcggat
     6121 cggtgtaata gaaaccgatc agcagatagg agcacaggcc cacgccttcc cagccgaggt
     6181 acatcagcag caggttatcg gcgagaacca gaactaccat gctggcgatg aacaggttgg
     6241 tgtaggcgaa gaagcgagag tagccctctt caccgcgcat gtaccaggag gcgtacatgt
     6301 gaataaggaa acccacacca gtgaccaccg agagcatggt cagcgacagg ccgtccagca
     6361 ccaggttaaa accgatgtta aagtcgccta ccgacatcca cgtccacagc ggctggctgt
     6421 atgactgctc gccgttagcg aagaaatcaa cgccgataaa ggcggttacc agcgccgcca
     6481 ggcccacaga gccaacgccg acgatcgccg aaacgttttc agaccagcgc ccacgggaga
     6541 acgccagcag gacgaagcca atcaatggca aaataatggt taaggcaagc atgttcatcc
     6601 gcgcatctca cttactgaat cgatgttcag gttctggcga cgacggtgaa gttgcagcag
     6661 cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg atggcgagaa tgtacatcac
     6721 ctgaccgtcg gtctgacccc agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt
     6781 aatcatgatt tccagaccaa tcagcataaa cagcagattg cgacggataa ccagaccggt
     6841 taagccaaga acgaataaga ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg
     6901 tgctcctccg tttttctttt cgcgctgtcg tctttacgat tgctcagcac ttcacccgca
     6961 cgctcttcac gaccgacgtg gaaggccaca accagacccg cgagcagcag catagaagcc
     7021 agttccaccg ccagtacgta aggcccgaac agcgtaatac ccactgcttt agcactgatt
     7081 ggcgtaccgt cgataccctg atcattaaca ccaaggatgg cgtaaacaat caccaccagc
     7141 atgatggccg acaaaattgc cggaccaatc cacacctgcg gtttcagcca ctggcgttcc
     7201 tgttcgattt ctgaaccgcc caggttgagc atcatcacca cgaacacgaa cagcaccata
     7261 atggcacccg cgtagacgat aatttccagc gcaccggcga agtaagcgcc cagtgagaag
     7321 aacaccccgg aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg taccggattg
     7381 gtatgggtga tcactcgcaa ggtcgcaagt atggctatca ggccacagat ataaaaagcg
     7441 aactccattg cccctctcct tacggtaaca ggctcttgac gtcgataggc ttggcttcgt
     7501 tctctgcttc gcccttatct ttgccgtcga ttgccatacc tgccatccgg tagaagttat
     7561 attccgggta tttgcccgga ccggagatca gcagatcctc tttttcgtaa accagatcct
     7621 ggcgcttgta ttcccccatt tcgaaatccg gggttaactg aatcgccgtg gtcggacagg
     7681 cttcttcgca cagaccacag aaaatgcagc gtgagaagtt gatgcggaaa aattccgggt
     7741 accagcgacc gtctttggtt tctgcttttt gcagcgagat acagccgacc gggcaggcta
     7801 ccgcgcagag gttacaggct acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga
     7861 tacgaccacg ataacggggc ggcagataga ccggctcttc cgggtacatt cgcgtttcgc
     7921 gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg aacctgggtg ccgaaaccta
     7981 ctaacaattc ttttaaggtc atggtcttat tgccccttat tgcgcctgcc agagaatgac
     8041 agccgccgtt accagcaagt tgatcagcgt cagcggcagg cagattttcc agccgaagga
     8101 cattacctgg tcataacgcg gacgcggtaa cgacgcacga atcaaaatga acatcatcat
     8161 aaagaacgcg gttttcagcg cgaaccagat gaatggcggt aacaacgggc cttgccagcc
     8221 accgaagaac agcgtcacca tcaatgcaga gatggtcaca atcccgatgt actcacccac
     8281 gaagaacaga ccgaacttca taccggaata ttcaatgtgg taaccatccg ccagttcctg
     8341 ctcggcttcc ggctggtcaa acgggtgacg gtgacatacc gccacgcccg cgatggcaaa
     8401 ggtaataaaa ccaaagaatt gcgggataac gttccacaca tgcgcctggc tgttgacgat
     8461 gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc atcagggaaa gcccgaggaa
     8521 cacttcgtag ctcagggtct gcgcagaagc acgcatcgca cccagcaacg agtatttgtt
     8581 gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa
     8641 gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc ggactgactg gcacaatcgc
     8701 aaaggccagc agcagcgagg taaaggcaat catcggtgcc agggtaaaga tgacgcgatc
     8761 cgagaatttc gggatccagt cttctttaaa gaacattttg atcatgtccg caaccagctg
     8821 gagcgaaccg ccccagccaa cacggttagg tccgtaacgg ttctggaaca gacccagcag
     8881 gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca accaccagca ggatcaccac
     8941 cgctttgagg atggtcagca ggatctcaat cagttccggt gatatccaac tcattgttgt
     9001 gcctccttga gatcctcaag atgcgcgcca gccagcaccg gagcaatgcc ggacataccc
     9061 atcggcaaac ccacctgccc tgccgtcagc ccttcagcga tttcaaccgg cagcgtgacc
     9121 gtgttgccat cgtaactaaa ggagacgcga gcgcctgcgt tcacgcccaa cttcgcggca
     9181 tccgctgggt tgagtttgat gtacggctgc ggcatacggc tctggaagac cggagcacgc
     9241 tgtgacaatt catcgctgcc aaacaggtgg taatacggcg cgatacgcca tttcccgtcc
     9301 tgcggctgga agcgtgccgg tacgctggtg aagtaatcca gaccattttc gctggtttca
     9361 aacagacgca cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg
     9421 ttccacgcct gcggggagtt ccagcccggc gcccaggcaa acggcacttg cgaacggtgc
     9481 gcagtcggct ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc
     9541 tgacgcggct catgaacgct gatattggcg cgcatggcgg tacgaccgct gtaacggtgc
     9601 ggttcacggg ccagtttctg cccacgaata cggaatgtcg catccggcgc agcatctttg
     9661 atacctgcca gttccgggat tttcgccaca acagcgtcaa tcacatggtc gagctgcgtc
     9721 cagtccactt cacggctcag cagggtgctg tgcagcgagt gtaaccagcg ccagctttcc
     9781 agcatgacag ttttgctgtc gtaataagca ggatcgtaaa cctggaagaa acgttgggcg
     9841 cggccttcgt tgttgatcac cgtaccgtcg ctttcagcaa agctggcagc agaaagtacc
     9901 agatgggcgt tttccataat cgctgtgcgt tgatgatcaa ccaccatcac cagcggcgct
     9961 ttagccagcg cagcattcac gcgggtagcg gaagcgtgac gatgcagatc gttttccagc
    10021 accaccaccg cgtcggcgcg tccggtttcc agttcggtca acgcttcttc aagcgaaccg
    10081 ccacccataa tgcccagccc catgctgttg acggaacggg caatcatggt gataccaacg
    10141 tcagcgccgc gacctttcag ggctttcgcg acgttagccg cagcctgaat cacctctatg
    10201 ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg cacctgccag tgcctgcacg
    10261 atgacgtcga ttttgctttg cagctcaggt tcgataccgt caaccgctgg cgcagagtta
    10321 tccagcgcat gggcgatggc aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa
    10381 gtccacgccg cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc
    10441 ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc agtcagccac tttctgtgct
    10501 gccgccattt cgcgcgcttt acctttcaca gcctgacgca ctgccagcgc gacgcgcgcg
    10561 ccggtctggg taacgtcttc gcccagcacc agtaccgcat cgtaagattc gatttcgcgc
    10621 agagccggag tataaatgcc gccttcacgc agcactttca gcgccagttg cagacgttcc
    10681 tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc ccaccagttc acgcagcgca
    10741 aagttgcttt ccacgctggc acgcggagaa ccaataccga tcactttctt cgactgacgc
    10801 agaatatctg ccgcgccctg cattgcctgt tcggcgttga gggtaatgaa atcatcgcca
    10861 cgacgctgta ctggctgacg cggacgatcc ttcaggttga cgtaaccgta accgaaacga
    10921 ccacggtcgc agaggaagta gtggtttacc gtaccgttgt aacggttttc gatacgacgc
    10981 agttcgccgt aacgttcacc ggggctgatg ttacagccga tggaacattg ctggcagatg
    11041 ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta
    11101 aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg
    11161 tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt aaacgcccag atctgtaccg
    11221 tcagcgtaat ctttgtagta acgcacacag cggtagcagg cgatgcagcg gttcatttcg
    11281 tgagagatga atggccccaa atcctgatta cggtgggtac gtttggtgaa acggtagcga
    11341 cggaagctgt gtccggtcat cacggtcata tcctgaagat ggcagttacc gccttcttca
    11401 cataccggac agtcgtgcgg gtggttggtc attaaccact cgaccacact ttcacggaac
    11461 tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat cggaagccgg tgtcatacag
    11521 gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac cgcacactgg
    11581 cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat atcaaggccc
    11641 agagacagac aagcttccag caggttgtcc gctccgttga cctcgtattc tttgccgtct
    11701 acatgaattg tagccattag catgcttcca gttttctcag tcagagactg agcgttaatc
    11761 gaaattcggt taccagcgct ctttcagcag gttcggctga atcccattaa tcaaatgggt
    11821 attgctgaac ggctgtttga ttcccgcctc aaattcttcg cggaaatatt tgatggcgct
    11881 ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa
    11941 tcgacacagt tgctcaagtg tttcgatatc gcccggctga ccttcaccac gctccagcgc
    12001 acgcagaatt ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc agccgcagga
    12061 ttcacgggcg aaaaactctt ccaggttacg caccagcgac accatgttga tctcatggtc
    12121 aaccgccatc gccagcgccg tacccagacg gcttcctgct ttaccgatac tttcgaattc
    12181 catcggcagg tcaagatgcg cttcggtcag gaagtcggtg cccgcgccgc ctggctgcca
    12241 ggctttgaat ttcagaccgt cacgcatacc accggcgtaa tcttcgagga tctcgcgcgc
    12301 ggtagtacca aacggcagtt cccacaggcc cggatttttc acccgaccgg agaagcccat
    12361 cagcttggtg ccagcatctt tacttttcga gatgttctga taccactcca cgccgttagc
    12421 gaggatcgcc ggaacgttac acagggtttc gacgttgttg acacaggtcg gtttacccca
    12481 tgcgccggag gtcgccggga atggaggctt cgagcgtggg ttagcacgac gtccttccag
    12541 ggagttgatt aacgctgttt cttccccgca gatgtagcgc cctgccccgg tatggacgaa
    12601 cagttcgaag tcaaaacctg ttcccataat atttttgcca agcagacctg cttcggtggc
    12661 ttcggcaatg gcacggcgta gattcactgc cgcttcgata tattcgccac gcaggaagat
    12721 gtagccacgg taagctttca gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg
    12781 cggcagttgc tccatcaaca ggcggtcttt ataggtgccc ggctccattt catcggcgtt
    12841 acacagcagg taacggatgt tcatggattc gtctttcggc atcaggctcc acttcaggcc
    12901 agtcgaaaag cccgcgccgc cgcgcccttt cagaccagcg tcttttacct gattaacgat
    12961 ttcgtccgga gacagcccgg tcagcgcctt acgcgcccct tcgtaaccat ttttgctgcg
    13021 gtattcgtcc agccacactg gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt
    13081 ttcgggagta cggataatgt ttttcattta taccgctcca gcagttcagg gatcgcttcc
    13141 ggggtcagat gcgcgtgagt gtcctcatcg atcatcatat ttggcccttt atcacagttc
    13201 cccaggcagc aggttggcag cagcgtaaag cgaccgtcaa acgtcgtttg ccctggtttg
    13261 atgttcagtt ttttctccag cgccgcctga ataccctggt aaccgttgat gtgacagacc
    13321 acgctgtcgc aataacggat cacatggcga ccaaccggtt ggcggaagat ctggctatag
    13381 aacgttgcca cgccttcgac atcgctcgcc ggaataccca gcacatcggc gatcgcgtgg
    13441 atcgcgccgt ccggcaccca gccacgctgc ttctgaacga ttttcagcgc ttcaatggac
    13501 gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca
    13561 ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt gcataattag cggtccacat
    13621 ctgacataac aaaatcgata ctgcccagat aaacaatcag gtcagacacc aggctgccgc
    13681 ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat
    13741 aactcatggt gctaccgtcg ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa
    13801 tcatctggaa agattcattg gcaggcatca ccggccccca cgacacttgc aggaagtggg
    13861 tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt
    13921 gatccgcttt gaacgggcct tccggcatgt tgttgaggca ctgctcaaga atgcgcagac
    13981 tctggcgcag ctcttccact ttcagcatta cgcgggtgta gcagtcagaa acaccaccac
    14041 caaccgggat ttcaaagtcg aagttttcat agccagaata aggacgcgcc ttacgcacgt
    14101 cgaagtcgat cccggtagca cgcaggcccg cgccagtggt gccccactcc agcgcctctt
    14161 tcgcgccata ggcggcaacg ccctgggaac gacctttcag aatggtattt tgcagcgccg
    14221 ctttctcgta agacgccaga cgtttcggca tccagtcgag gaactcacgc agcaggcgat
    14281 cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac
    14341 ggaaacccgt gatcgcttcc accagatcgt aaattttctg acgatcggta aaggcgaaga
    14401 acacgggcgt cattgcgccg acgtcctgaa taaaggtcga gatgtacagc aggtggctgt
    14461 tgatacggaa cagttcggag agcataacgc gaatgacgtt aacgcgatcc ggcacggtga
    14521 tcccggccag tttctctacc gccagcacgt aaggcatttc gttaacgcag ccgccgaggt
    14581 attcaatacg gtcggtatac ggaatgtagc tgtgccagga ctggcgctcg cccattttct
    14641 ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga
    14701 gttgtaaaac gatacggaaa gccccgtgcg ccgacgggtg gttcggaccg aggttgagga
    14761 acatgaagtc ctcgttttcg gtgccgcgct tcatccccca ctcttccggt ttgaaggtca
    14821 gggcttccat ctccagatcc tgtttggctt tggtcagctc aaacggcgag aattcggtag
    14881 cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca ggtttgcggc atcatgatgc
    14941 ggcgcaggtt cgggtgaccg tcgaaagtaa tgccaaacag atcccaggtt tcacgctcat
    15001 accagttagc gttcgggaac agtttggtga aggtcggtac gtgcaggtcg ttttctgcca
    15061 gcgccacctt cagcatgatg tcgcggttac gatcgataga aatcagatgg tagaaaacgg
    15121 aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta
    15181 agtcaaacag catgacgtaa ggtttcggca gtttctttaa gaaatcgcca acttccagta
    15241 attgttcacg cttgatccac acaacgggaa ccccggtgcg agtcgcctga acagtaaagg
    15301 catccggccc aaaacggttg cgcagttcgc caattaccgg atcatcaaga tgatcgcggg
    15361 tctgccaggc gggttcttgc gcggttaagt cggtcatatt gttcaccatt gcaaatggtc
    15421 cgtggtgact gtcggcgcag taagcttcgc gaaatagatt taggaataag cgaagaaaaa
    15481 tccctttgcc gacaggcgca aattaaatct cgtcaggtgt acgcaggttg gttacggcaa
    15541 tacgttcacc gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa acgccctgat
    15601 cgccaaccac ccaggagagc ggacgacgtt ctttgccgat agattcctgc aacagcatca
    15661 gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg gatatacaca tcaaccggga
    15721 tgaatttatc gacgccctgc acaacggaat aaatatcgta cataccacca gagttggcac
    15781 aggcacccat tgagataacc cattttggtt ccagcatctg gtcatacaga cgctgaataa
    15841 ccggtgccat tttggtaaag caggttcctg caaccaccat caggtcagcc tgacgcggcg
    15901 aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca
    15961 ccatctcaac gtaacagcag gaaagaccga agttatacgg ccaaattgag tttttacgac
    16021 cccagttaac catgtcattg agcttgccca taaacacgtt tttgttaact tcttgctcca
    16081 gagggtcggt tacgatctcc tgcttttgca gggggtaacg gtcgttctca ccgttgggat
    16141 ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga cgattagcga
    16201 tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg
    16261 cgccaatacg caccagataa accagacctg ccagtaacac aaaaataaaa attgcagctt
    16321 ccacaaagcc tacccagccg ctttcgcgga tagaggttga ccatgcgaac agatacagcg
    16381 cttcaacgtc gaagataacg aagaacatgg ccaccagata aaacttggcg gacaggcgta
    16441 agcgggcgga gccgaccgag tcgataccgg attcaaacgg cacgtttttc gacctcgcgc
    16501 gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag gcaacacagg ccaatggcaa
    16561 cgataagaaa gatagcgaat gcccagtgat gagcgatgac ttcagtggat gttgacatac
    16621 tcattgctta ctcatcaaaa gtagcgccag attcactgct cttcacggca gatggacgcc
    16681 acatcgattc atggggagga ataaaaaaaa ccttacaatc actgtagaaa ttcttttata
    16741 cagctaattg atgtggtctt ttactccttt ctataacctt ttgtcaactt taacaaaagt
    16801 ttcttcacat tagtttacat aatatcaaca ccattagcat ttaatgccct ttcaccccag
    16861 atccttgacg actccaggat aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg
    16921 aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt cctcccccca aaagagagta
    16981 ttttcttgat ctgtgacacg cttttgtcat tccataacaa aaacgcagca acaaatttac
    17041 gtatttttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    17101 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    17161 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgttttttac
    17221 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    17281 atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    17341 ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    17401 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg
    17461 ggctcatcat ctcaaccggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    17521 cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg
    17581 tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga gggatcggct
    17641 cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gttcgcaggt
    17701 tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt
    17761 ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat
    17821 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    17881 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    17941 taaaacgcag gattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    18001 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    18061 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    18121 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    18181 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    18241 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    18301 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    18361 atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta agttactcac
    18421 tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac
    18481 tctcgtgcag catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat
    18541 caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag
    18601 ggtcattgcg aattatctga tgcaaagtta tgttatgtta ggcaaagtaa
//
LOCUS       DAZFKM010000097        17989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFKM010000097 DAZFKM010000000
VERSION     DAZFKM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..747
                     /locus_tag="ACHKAY_003461"
     CDS             40..747
                     /locus_tag="ACHKAY_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5702076.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            744..2195
                     /gene="djlC"
                     /locus_tag="ACHKAY_003462"
     CDS             744..2195
                     /gene="djlC"
                     /locus_tag="ACHKAY_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO5702077.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2255..3925)
                     /gene="hscC"
                     /locus_tag="ACHKAY_003463"
     CDS             complement(2255..3925)
                     /gene="hscC"
                     /locus_tag="ACHKAY_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO5702078.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4009..4944)
                     /gene="rihA"
                     /locus_tag="ACHKAY_003464"
     CDS             complement(4009..4944)
                     /gene="rihA"
                     /locus_tag="ACHKAY_003464"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO5702079.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5062..5787)
                     /gene="gltL"
                     /locus_tag="ACHKAY_003465"
     CDS             complement(5062..5787)
                     /gene="gltL"
                     /locus_tag="ACHKAY_003465"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO5702080.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5787..6461)
                     /gene="gltK"
                     /locus_tag="ACHKAY_003466"
     CDS             complement(5787..6461)
                     /gene="gltK"
                     /locus_tag="ACHKAY_003466"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO5702081.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6461..7201)
                     /gene="gltJ"
                     /locus_tag="ACHKAY_003467"
     CDS             complement(6461..7201)
                     /gene="gltJ"
                     /locus_tag="ACHKAY_003467"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO5702082.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7371..8279)
                     /gene="gltI"
                     /locus_tag="ACHKAY_003468"
     CDS             complement(7371..8279)
                     /gene="gltI"
                     /locus_tag="ACHKAY_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO5702083.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8734..10611
                     /locus_tag="ACHKAY_003469"
     CDS             8734..10611
                     /locus_tag="ACHKAY_003469"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO5702084.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10689..12227)
                     /gene="lnt"
                     /locus_tag="ACHKAY_003470"
     CDS             complement(10689..12227)
                     /gene="lnt"
                     /locus_tag="ACHKAY_003470"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO5702085.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12252..13130)
                     /gene="corC"
                     /locus_tag="ACHKAY_003471"
     CDS             complement(12252..13130)
                     /gene="corC"
                     /locus_tag="ACHKAY_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO5702086.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13220..13687)
                     /gene="ybeY"
                     /locus_tag="ACHKAY_003472"
     CDS             complement(13220..13687)
                     /gene="ybeY"
                     /locus_tag="ACHKAY_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO5702087.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13684..14724)
                     /locus_tag="ACHKAY_003473"
     CDS             complement(13684..14724)
                     /locus_tag="ACHKAY_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO5702088.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14877..16301)
                     /gene="miaB"
                     /locus_tag="ACHKAY_003474"
     CDS             complement(14877..16301)
                     /gene="miaB"
                     /locus_tag="ACHKAY_003474"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO5702089.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16447..17622
                     /gene="ubiF"
                     /locus_tag="ACHKAY_003475"
     CDS             16447..17622
                     /gene="ubiF"
                     /locus_tag="ACHKAY_003475"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO5702090.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17776..17850)
                     /locus_tag="ACHKAY_003476"
     tRNA            complement(17776..17850)
                     /locus_tag="ACHKAY_003476"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17816..17818),aa:Gln,seq:ctg)
     gene            complement(17899..17975)
                     /locus_tag="ACHKAY_003477"
     tRNA            complement(17899..17975)
                     /locus_tag="ACHKAY_003477"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17939..17941),aa:Met,seq:cat)
BASE COUNT         4389 a         4516 c         4676 g         4408 t
ORIGIN      
        1 aaaagtagtc agttaatgca cttaagggat tattaacaaa tgaataagga agaacaatat
       61 cttctttttg cgctttctgc gcccatggaa atacttaatc agggttgcaa acccgcgcat
      121 gactcgccaa aaatgtatac cgggataaaa gaattcgatc tctcatcatc ctggggaata
      181 aataatcgtg atgatttaat tcagactatt tatcagatga ctgatgatgg tcatgccaat
      241 gatttagctg gcttatatct gacatggcat cgctcttccc ctgaagagtg gaaagcatta
      301 attgctggtg gctcggagag agggttgatt tatacccaat tcgttgccca aaccgctatg
      361 tgctgtggag aagggggaat caaagcctgg gattatgttc gaatggggtt tctgtcacgg
      421 gtcggtgtgc tcaacaactg gctgacggaa gaagagagtt tatggctgca atcgtgcgtc
      481 tatgtacgag ctcaccatta ctatcacagc tggatgcact atttttccgc ctattcgtta
      541 ggacgtctct actggcaatc ttctcagtgc gaagacaaca cttcgctacg cgaggcgcta
      601 accctgtaca aatacgatag cgccgggagt cggatgttcg aagagctggc agcgggaagc
      661 gatcgtttct atgccacgct tccctggcag ccattaactg tccagcctga atgcccggta
      721 acgcttaagg atgtgagcga cctatgaaaa catgttggca aatcctcgaa attgaaagca
      781 cgacgcaaat agacattatc cgccaggctt atcttgctcg cttaccgtta tgtcatcccg
      841 aaaccgatcc gcaggggttt aaagcattac gccaggccta tgaagaggcg ctgcgactgg
      901 cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga tgccgccgct gaacatgaaa
      961 tactgcgtgc attcaggaca ttactggatt cagaaagtga tcgttttcag ccctccgcct
     1021 ggcagaaatt tattcagcaa ttaaatacct ggaacatgga ggatgtcgat caattacgct
     1081 ggccgctgtg tgcaatcgct atagaagcgc gatatctttc attaaattgt gcttctttgc
     1141 tggcagagcg tttgaactgg cattcgttta atgacagcga aggaatggat gaggaagaaa
     1201 gggaggcttt tcttgaggcc attcaggctg gtgattgttt cgatttcctt agccttctgg
     1261 aatatcccgt tgcgttgcag aaccagactg ttgagtatta cttcgcgctg gaacgttgct
     1321 gccgttacca tcctgactac gtcactgcgt ttttggcgat ggaaggtccg tggttcattc
     1381 ctgatgatgc aaagttacat cgcaaactgt tgcgctggta cagctcggtg caaacaggta
     1441 tggcggaact cattcctgta gccaaacagt ggcaaatgga agaaccagaa agcgaagatg
     1501 cccgatatta cttgtgtgct caaaggttgt actgcggcga gggggaaagc ctgcttgccg
     1561 atctctgcgc gtaccgggaa agttacccat ctacacaagc tgataatttg ttgttgcagt
     1621 ggagcaagcg tcattgcccg gattatttcg cgttattagt gatggttatc gaagcgcgga
     1681 gcatggtaga tgcgcaaggt caaccgctga aatatgttcc tggtgagagc gcccggacgc
     1741 ggctgttatg ggcggaaatt ttacatagcg gaaaattatc gccgttaggt caatcgttta
     1801 ttgagtcgtt attcttcaaa cgcaaagcat gggcgtggtg gaaatcgaga gtcggtagcg
     1861 agacagaaga agattcaccg ttactggatt tgtatcgggt agcggaacag gtagtacttg
     1921 aagcgtttcc gaaacaagag atgctggccc gtcttaatac aaggctggaa ggcggagatg
     1981 ctcatccatt agaggccatt atcacccgga tgctgttgac gaaagtaaaa ctcgagccgg
     2041 aggatgaaga tgtcgatgag ccaacacctg aaaaccatga agaaaaaaat gatgagggtg
     2101 aaaaaccaca gagcattacc agcattatca aaatcagttt aacggtgctg gttataggtt
     2161 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
     2221 gatgcagcat ttacgccgca tccggcagcc gtgattatga ggtctcaatg gctaaataaa
     2281 cgctcgcccg ccgcctgacc tcatcgacgc gggccatatc gtttgactgc aacgccgcat
     2341 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgcc cgcgcccaca
     2401 actcttccag tttggcctta aaggtgcggt tgatgagcat atcgcgcgga taaattttca
     2461 atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc gctcaatgtt accgggctat
     2521 gattaatgac tctggactta acgctgccat cttccagaag cacgtcaact tcaagcaagc
     2581 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
     2641 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
     2701 cgttaaccgt aattgaattc tgttccgggt gcatggtcga gtaagtttct acacgcgaca
     2761 cgggtactgt ggtgtttcgt tcaataattg gcgagaaaat gccggaaacg ccctggcggt
     2821 taacttcaac gcccaacgag taagggcaaa tatcagtgag gatcacctct tcaatatctt
     2881 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcgggt
     2941 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
     3001 tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgtgcat
     3061 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
     3121 ccagttcgtt ctcgtaaaat tcgcactccc gcgtttcatc ctggtactgc cagcgaatgt
     3181 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctcccagtt
     3241 cactctcgtt aagcgtggtc ctggcgacat ccgcgcgttt taaaacctca tcgaccagca
     3301 tatgggtaaa atcttcgcca ccaagaaagt tgtcgccagc ggaggcgtga acttcaatca
     3361 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaacacca
     3421 gcgaacgggt attttgttgg gtatgcaggc catacgccat cgcagctgct gtgggttcat
     3481 taattaagcg taccgcattt aacccggcta actccgctgc taaacgggta tgcttgcgtt
     3541 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     3601 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     3661 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcacgttta aataacgctg
     3721 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     3781 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     3841 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     3901 caatggcgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     3961 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     4021 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     4081 ggcattcggt ttattgccag tcagataata ataatcaaca accgtcatac cctggctata
     4141 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     4201 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     4261 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     4321 aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgt gctttgtgag taacatccag
     4381 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     4441 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     4501 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     4561 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     4621 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     4681 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     4741 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     4801 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     4861 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     4921 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     4981 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     5041 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     5101 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     5161 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     5221 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     5281 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     5341 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     5401 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt
     5461 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     5521 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     5581 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     5641 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     5701 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     5761 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     5821 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaataggat catctcaacc
     5881 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     5941 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     6001 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     6061 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     6121 tctgaatagt aggccgcttc aaacattgaa aacgccacca tcgccgaaat taaacggata
     6181 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     6241 cacagcaaaa ccataactaa aggaatcgag cggaatacgt taacgtaggc tttggcaaac
     6301 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     6361 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     6421 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     6481 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataacc gcgttaatca
     6541 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     6601 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     6661 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     6721 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     6781 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     6841 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     6901 taaaccacat accgattttc tctggcagca actccgggat caccagatac caggtaaaga
     6961 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     7021 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     7081 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     7141 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     7201 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     7261 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     7321 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     7381 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     7441 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     7501 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     7561 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     7621 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     7681 acggcacgac cgctttccag ggtacggaaa gagtcaccgt gatctttggc gctaatgatg
     7741 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga agtagtgccg
     7801 gaagtgacga ctacggcttt gcctttcagg tcggcaaaat ctttgatatc gccgcccttt
     7861 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     7921 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     7981 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     8041 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     8101 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcacg
     8161 ccgtttttgg cgattttgtc cagcgtactg cccgctaccg gggcggcgtc atctgcctgt
     8221 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     8281 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     8341 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     8401 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     8461 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     8521 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     8581 atacttaggg gcgaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     8641 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     8701 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     8761 aatgttcaat tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     8821 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggt
     8881 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     8941 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     9001 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     9061 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgaaa gtgagcagat agtcatcagc
     9121 gttggggcat ccggggcgat tctgggaata gctggtgcag cgatagcaac acagtttgct
     9181 tcaggtacgg gcacatacca taaaaaccag cggcgagtat ttcctctgtt gggtatggtg
     9241 gcgctgacac tgttgtacgg tgcccggcaa acaggaatag ataacgcttg ccacattggc
     9301 ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaacaga
     9361 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     9421 cttgcgcagc agcagatgga tgagtcagtt ttacaggtca ggcaaagcct gcgtgaagcg
     9481 ttttatccgc aggagattga acaagagcga cgacaaaaaa aacaacagtt agcggaggaa
     9541 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     9601 ttactcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatacgttg
     9661 tatgccgcaa ttgaaaacac caacagcatt gttgttttcg acctcggaca aaagaaaatc
     9721 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     9781 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgacg
     9841 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtca
     9901 attaccaccg gtgcacatcc tgacagtttt atcctctcgc gtgatggcac aaaagcctgg
     9961 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg
    10021 gcggatatcc cgctggagaa atacgacggg acggggacga gcggtaaacc aggcgcctgg
    10081 gttatggcac tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac
    10141 atcgtgcgga tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt
    10201 ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgccgatagt
    10261 caggggattt cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt
    10321 agcaggagcg tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg
    10381 tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc
    10441 gtttatcctg ccagctacgt taatcacctt cgtttttcag cggatggtag aaaaatattt
    10501 gtcatggcga agaacgggtt gatccaaatg gacaggacgc gctcgcttga tccgcaggca
    10561 attattcgtc atccccaata tggcaatgtg gcttgtatcc ctgaaccgta agaaaaaggg
    10621 gcatgataac catgccccaa agctcaatga gaaagtacgt tttgctcatg caggatacat
    10681 caggcacatc aacgtttacg tgctcgcaga ctcatcagca cagcggcaaa accaaacagc
    10741 gccgtcagca cccacagcgg ccagttgccg gtacgtgcat acggtgtgag tccggtggtc
    10801 ggtgtcacat tagtggtcaa cacctcgcgg gtgaactgcg ggatcatcgc ctgaatctca
    10861 ccctgcgggc caatcaccgc cgtaatgccg ttgttggtgc tgcgcaacag tgggcgcgcc
    10921 agctccagcg cacgcattcg cgccatctgg aagtgctgcc atggaccaat agacttacca
    10981 aaccacgcat cgttggagat agtcagcaga tagtcggtat ccgggcggaa gttatcgcgc
    11041 acttgctcgc caagaatgat ctcgtagcaa atggccgcag taagctcaat accatttacc
    11101 gacagcggcg gctggatata tggcccacgg ctgaacgacg acatcggcag atcaaagaac
    11161 ggtgctaacg gacgcagaat cgactccagc gggacaaact cgccaaacgg caccagatgg
    11221 tttttgttat agcgatcggc tgattcgtag ctgtacggtg cacctttacc cagcgtgatg
    11281 atggtgttgt aggtatcgta gcggttctgc ttattgagac gcgcgtcgac aatcccggtt
    11341 accagcgagc tacctttatc acgcaactca ccgtccagtg ctttgaggaa cggttgctga
    11401 ttaatttcca gatcggttat cgccgactcc ggccagataa tcaacgatga tttgcccagc
    11461 agcggtgccg ttgcgttgta gtaaatcttc agcgtattaa gaagctggtc tccgtcccat
    11521 ttcagtgatt gcggaatatc gccctgaacc atcgaaacct gaatggtttt ctccggttgc
    11581 ggggtaaacc attggatgta acgcagcggg aagggaaggg caaacagcac gacggccacc
    11641 accagcggac gccagttgcg tttgactaac gccagtgcca gcaggccgct aaccatcatc
    11701 agcaggaagt taatggcttc cacgcccatt attggtgcca gcccttttaa cgggccatca
    11761 atctggctat agccgaactg taaccacggg aagccagtca gcacccaacc gcgcagaaac
    11821 tcggtcactt gccagagagc aggggcggca atcgccacgc gcagccaggt ggttttcggc
    11881 cacaggcgcg acagcacgcc agcaaacagt ccggtataca gcgacaaata cgccgccagc
    11941 agcaccacca ggaagatgtt aaccgggcca ggcatcccgc caaaggtcgc gatgctgaca
    12001 tagacccagt taataccggt gccaaagagg ccaaatcccc agcagaagcc aatagcggca
    12061 gactggagcg ggcggcggtt aaaggtcagc gcctgaagcc ccatcagcga aacaatcgcc
    12121 gcaggccaga catcgtaagg agagaaggcc agcgttccgc aggcaccgaa taataacgcc
    12181 agcagcaggc gaatgcgctg gcgttcaatt aatgaggcaa aagccatgta gttatctatc
    12241 cagtttcggt tttattcatc cagctttggc tggggtgagt catccgggat tttgacatga
    12301 acctgaataa tacgccgact atcggccatc gccactttga actggtaacc gtcgatgtcg
    12361 atagtttcgc cacgcgccgg aagatgccca aacgcctgca tcaccagacc accgatagtg
    12421 tcgacttctt catcgctaaa gtgggtgccg aacgcttcgt tgaagtcttc aatggaagcc
    12481 agtgcgcgca cggtccaggt atgacgactc agctgacgga agtcgatatc atcttcttcg
    12541 tcatactcgt cttcaatctc accaacaatc agttccagga tgtcttcaat ggtcaccaga
    12601 ccggaaaccc caccgaattc gtcaataacg atcgccatgt ggtaacgctg agagcgaaac
    12661 tctttcagca tccggtctac gcgcttactt tcaggaacga caaccgcctg acgtaacact
    12721 ttgtccatgc tgaaggcttc agcatcgctg cgcataaacg gtagcaagtc tttcgccatc
    12781 agaatccctt caatgtgatc tttgtcttcg ctaatcaccg ggaaacgtga gtgggcggac
    12841 tcgatgatga catcaaggca ttcgtccagc gtctggttgc gtttcagggt aatcatctgg
    12901 gagcggggga tcatgatgtc gcgaacgcgt tggtctgcga tgtccatcac cccttcgagc
    12961 atatcgcgcg tatcttcgtc gataaggtcg ttctgcccgg aatcacggat cagcgccagc
    13021 agttcgtcac ggtttttcgg ttcaccgtgg aaaagttggc tgagtaacag ggagaaaaat
    13081 cccttcttgt tgcttatcgt gtcactactg tgtgaattgt cgtcgctcat ggcgtcgtat
    13141 gggttctcat gttagttaat caaaacgccg tcgttaatca ccaacggcgg ggacgtctgc
    13201 cagtcaaatg cctggcaaat tattctttct cggcaatgta cggatcctca tagcccagag
    13261 caagcataat ctctgtttcg agggcttcca tttcttctgc ttcgtcatct tcgatgtgat
    13321 cgtaacctaa caaatgcaga ctgccgtgca ccaccatatg cgcccagtgc gcctccagtg
    13381 gtttgccttg ctcctgagct tccttctcaa ccacctgacg gcagataacc agatcgccca
    13441 gtagcgacat ttccatgcca ggcggcactt caaacgggaa ggagagcacg ttggtcggct
    13501 tatccttacc gcgataggtc agattcagac tgtggctttc ggcggtatcg accacgcgaa
    13561 tagtcacttc cgattcttcc tgaaactgcg ggatcaccgc attcagccat gtttgaaact
    13621 ggctctcttc cggtaacccg gaattatctt cacatgccag ttgtaaatcg aggatcacct
    13681 gactcatttt tgttcctgtt cttcgcgctt gcgttctgcc gccagcgccg cttttcgttt
    13741 ttgttcggct tcttcccagg cttcataggc gttaacgata cgcgccacca ccgggtgacg
    13801 aaccacgtct tcgctgtgga agaagttaaa gctgatctct tcgacatcgg ccagcacttc
    13861 gatggcgtga cgtaagcctg atttagtatt acgcggcagg tcgatctgtg tgacgtcgcc
    13921 ggtgataacc gcttttgagt taaaaccgat acgggtcagg aacatcttca tctgttcgat
    13981 ggtggtgttc tggctctcat cgagaatgat aaacgcgtcg ttcagcgtac gaccacgcat
    14041 ataggccagc ggcgcgactt caataacgtt gcgctcaatc agtttctcaa ctttctcaaa
    14101 gcccagcatt tcaaacagcg cgtcgtacag cgggcgcaga tacgggtcta ctttctggct
    14161 taaatcgcca ggcaggaagc ccagtttctc accggcttcg accgccggac gagtcagcag
    14221 aatacggcga atctcctgac gctccagggc atcaactgcc gcagccactg ccaggtaggt
    14281 tttacccgta cccgccgggc caacgccgaa ggtaatgtca tggtcgagaa tattggcgat
    14341 gtactgcgcc tggtttggcg tgcgcggctt aattacgccg cgtttggttt tgatattgac
    14401 cgctttgccg tactccggca cgctctccgc actttgctcc agtactcgcg cttctttaat
    14461 cgcaaggtgg atctgttccg gttcgatatc ctgaatctga ccgcgcatcg gggctgtatc
    14521 gacatacagg ctacgcagaa tgtctgccgc agcggtgacg caaatcggac ggccggtcag
    14581 tttaaagtgg ttgtcgcggc gattgatctc gatgccgaga cggcgttcga gctgcttgat
    14641 gttgtcatca aacgggccgc acaggctcaa cagacgcgcg ttgtctgctg gctccagggt
    14701 gatttcgcga gtgtctatgt tcaaaccgtt cctcttatct gtatgtcgcc ggaagctgaa
    14761 cattcaccgg cctataagga aattattcac gccacaggaa aaaggcgcaa gcattgcgat
    14821 aaagatgggg ataaagagag aaaaaacaag gcccaccgga acggcgggcc tgagaattac
    14881 ggctgataat aacccacgcc aaggtcgttt tctttgcggg tacgggcaat caccgattcc
    14941 ggtgtttctg ccacgcgcag acccatttca tcttcagtac gcaccacttt accgcgcaga
    15001 gagttcgggt agacgtcggt aatttctaca tcgacgaatt taccgatcat atccggtgtg
    15061 ccctcgaagt tgaccacgcg gttattttcg gtacgcccgg aaagctccat gatgctctta
    15121 cgcgaagtcc cttctaccag aatacgctgg gtggtgccga gcatccggcg gctccatgcc
    15181 atcgcttgct gattgatacg ttcttgcaga atatacagac gttgcttctt ctcttcttcc
    15241 ggaacatcat caaccatatc ggcggctggt gtacccggac gtgcagagaa gataaagctg
    15301 tagctcatgt cgaaattgac gtcggcaatc agcttcatcg ttttctcgaa gtcttcggtg
    15361 gtttcgccag ggaagccaac aatgaagtca gaactgatct gaatatccgg acgcgccgca
    15421 cgcagtttac ggatgatcgc tttgtactcc agtgccgtat gagtacggcc catcaggttc
    15481 agaatgcgat cggaaccgct ctgtaccggc agatgcagga agctcaccag ctccggcgtg
    15541 tcgcgataca cttcgatgat atcgtcggtg aattcgatcg gatggctggt ggtaaagcga
    15601 atacgatcga tcccgtcgat cgcagcaacc agacgcaaca gatcggcaaa cgatccggtg
    15661 gtgccgtcgt agttctcacc acgccaggca ttcacattct gaccgagcag gttgacttca
    15721 cgcacgccct gagccgcaag ctgggcgatc tcaaacagaa tatcgtcgga cggacggctt
    15781 acctcttcac cacgggtgta aggcaccacg cagtaggtgc aatatttatt gcagccttcc
    15841 atgatggaga caaacgcggt cggcccttcg gcgcgcggtt ccggcagacg gtcaaacttc
    15901 tcgatttccg ggaagctgat atctacaacc gggctgcggt cgccgcgcac ggagttgatc
    15961 atctccggca gacggtgcag cgtttgcggc ccaaaaataa tatcgacata gtgggcgcgc
    16021 tggcgaatgt gctcgccttc ttgcgatgcc acgcagccac cgacgccgat aatcaggtct
    16081 ggattcttct cttttaacag tttccagcga cccaactgat ggaagacttt ttcctgagcc
    16141 ttctcgcgga ttgagcaggt gttcagcagc agcacatccg cttcttccgc cacgtcggtc
    16201 agttgatagc cgtgggtggc atccagcaga tcggccatct tcgatgaatc gtactcgttc
    16261 atctgacagc cccaggtttt aatatggagt tttttggtca tcgacttgct cttgcgaaat
    16321 agtagccagg aatgcagggc gcatagtgta atgctttgct gccgttgtga ccagtatgag
    16381 cgttatcagc ccttgggggt aaaaatcctg taaacttaaa gcatattgct aacaggatga
    16441 ttgaccatga caaatcaacc aacggaaatt gccattgtcg gcggaggaat ggtcggcggc
    16501 gcactggcgc tggggctggc acagcacgga ttttcggtaa cggtgatcga gcacgcagaa
    16561 cctgcaccgt ttgtcgctga tagccaaccg gacgtgcgga tctcggcgat cagcgccgca
    16621 tcggtatcat tgcttaaagg gttaggggtc tgggatgcag tacaggctat gcgttgccat
    16681 ccttaccgca gactggaaac gtgggagtgg gaaacggcgc atgtgatgtt tgacgccgct
    16741 gaacttaagc tgccgctgct tggctatatg gtggaaaaca ccgtcctgca acaggcattg
    16801 tggcaggcgc tggaagcgca tccgaaagta acgttacgtg tgccaggctc gctgattgca
    16861 ttacatcgag ataatgatct tcaggagctg gaactgaagg gcggtgaagt gattcgcgcg
    16921 aagctggtga ttggtgctga cggcgcaaat tcgcaggtgc ggcagatggc gggaattggc
    16981 gttcatgcct ggcagtatgc gcagtcgtgt atgttgatta gcgtacagtg tgagaacgat
    17041 cccggcgaca gcacctggca gcaatttacc ccggacggac cgcgtgcgtt tctgccgttg
    17101 tttgataact gggcatcgct ggtgtggtat gactctccgg tacgtattcg ccagttgcag
    17161 aatatgaata tggcgcaact ccaggcggaa atcgcgaagc atttcccgtc gcgtctgggt
    17221 tacgttacac cgcttgccgc tggtgcgttt ccgctgacgc gccgacatgc gttgcagtat
    17281 gtgcagccgg ggcttgcgct ggtgggcgat gccgcgcata ccatccatcc gctggcgggg
    17341 caggggggga atcttggtta tcgtgatgtc gatgccctga ttgatgttct ggtgaatgcc
    17401 cgcagctacg gtgaagcgtg ggccagttat cctgtcctca agcgttacca gatgcggcgc
    17461 atggcggata acttcattat gcaaagtggt atggatctgt tttatgccgg attcagtaat
    17521 aatctgccgc cgctgcgttt tgtgcgtaat ctcgggttaa tggcggcaga gcgtgctggc
    17581 gtgttgaaac gtcaggcgct gaaatatgcg ttagggttgt agccttacaa cattgccgga
    17641 tgcgtgccaa ccgtaggtcg gataagacgc ggcagcgtcg catccgacat tgaaggataa
    17701 gacgtgtcaa catcgcattc gacattgaat gaacgcagaa aagcaaaaag ctcgccgaag
    17761 cgagcttttt taatgtggct ggggtacgag gattcgaacc tcggaatgcc ggaatcagaa
    17821 tccggtgcct taccgcttgg cgatacccca acaaattggt ttttgaattt gccgaacgta
    17881 ttcgacacat tcagaatttg gtggctacga cgggattcga acctgtgacc ccatcattat
    17941 gagtgatgtg ctctaaccaa ctgagctacg tagccagatt gtttcttcg
//
LOCUS       DAZFKM010000098        17762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFKM010000098 DAZFKM010000000
VERSION     DAZFKM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..548)
                     /gene="ybcV"
                     /locus_tag="ACHKAY_003478"
     CDS             complement(138..548)
                     /gene="ybcV"
                     /locus_tag="ACHKAY_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO5702091.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            849..1055
                     /locus_tag="ACHKAY_003479"
     CDS             849..1055
                     /locus_tag="ACHKAY_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702092.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1628..2155
                     /locus_tag="ACHKAY_003480"
     CDS             1628..2155
                     /locus_tag="ACHKAY_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO5702093.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            2164..4263
                     /locus_tag="ACHKAY_003481"
     CDS             2164..4263
                     /locus_tag="ACHKAY_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5702094.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            4260..4472
                     /locus_tag="ACHKAY_003482"
     CDS             4260..4472
                     /locus_tag="ACHKAY_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702095.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4472..5980
                     /locus_tag="ACHKAY_003483"
     CDS             4472..5980
                     /locus_tag="ACHKAY_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5702096.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5925..7952
                     /locus_tag="ACHKAY_003484"
     CDS             5925..7952
                     /locus_tag="ACHKAY_003484"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO5702097.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            8039..8362
                     /locus_tag="ACHKAY_003485"
     CDS             8039..8362
                     /locus_tag="ACHKAY_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO5702098.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            8355..8630
                     /locus_tag="ACHKAY_003486"
     CDS             8355..8630
                     /locus_tag="ACHKAY_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO5702099.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8642..9220
                     /locus_tag="ACHKAY_003487"
     CDS             8642..9220
                     /locus_tag="ACHKAY_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702100.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9217..9618
                     /locus_tag="ACHKAY_003488"
     CDS             9217..9618
                     /locus_tag="ACHKAY_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5702101.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9629..10372
                     /locus_tag="ACHKAY_003489"
     CDS             9629..10372
                     /locus_tag="ACHKAY_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702102.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10433..10819
                     /locus_tag="ACHKAY_003490"
     CDS             10433..10819
                     /locus_tag="ACHKAY_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5702103.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10828..11157
                     /locus_tag="ACHKAY_003491"
     CDS             10828..11157
                     /locus_tag="ACHKAY_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5702104.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11129..14194
                     /locus_tag="ACHKAY_003492"
     CDS             11129..14194
                     /locus_tag="ACHKAY_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5702105.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14194..14523
                     /locus_tag="ACHKAY_003493"
     CDS             14194..14523
                     /locus_tag="ACHKAY_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702106.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14533..15231
                     /locus_tag="ACHKAY_003494"
     CDS             14533..15231
                     /locus_tag="ACHKAY_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5702107.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15236..15979
                     /locus_tag="ACHKAY_003495"
     CDS             15236..15979
                     /locus_tag="ACHKAY_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5702108.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15877..16524
                     /locus_tag="ACHKAY_003496"
     CDS             15877..16524
                     /locus_tag="ACHKAY_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5702109.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16585..>17762
                     /locus_tag="ACHKAY_003497"
     CDS             16585..>17762
                     /locus_tag="ACHKAY_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702110.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     "
BASE COUNT         4289 a         4091 c         5308 g         4074 t
ORIGIN      
        1 aaatttatcc ataaacctcg ttttttcggg gttttgttat atttgaatgg ttccgaatat
       61 ctaaatcaca attgttgatg gtttttatta aaccaatgca gtccggctca ggagtgagag
      121 aagccggacg ttatggttta gcgtggtaag atctgtgtag ttttctggat gctttcagta
      181 aatagtaatg agttatcaaa ggcatagtaa tatctttggt gttcctggat atttgtaacc
      241 catcggaaaa ctcctgcttt agcaagattt tccctgtatt gttgaaatgt gatttctttt
      301 gatttcaact tattatagga ggtctctata agatgtttgt ttctggagaa tttaacattt
      361 acaacctttt tgagtccttt tactaacact atgttgtcgt tttctaacac aatgtgaata
      421 ttatctgtgg ctaaatagta aatataaagt gagacattgt gacgttttag ctcagaataa
      481 aataattcac agtttaaatc tttacgcact tgatcgaata tttctttaaa aatggcagcc
      541 tgagccattg gtaaaccttc catgtgatac gatggcgcgt agttagcatt atcgtgttta
      601 ttgtttcaat ctggtctgac ctctttgtgt tttgttgatg atttatgtca aatattaagc
      661 ctgtttttaa tgaatagtgt tgattgcgta acaaaattga gccttgctgg catccaggag
      721 ggatatgcaa ccgacagatg tatgtaaggt cgatgtactc aaactttcat acttttcctc
      781 ttttatgcag taagatttga agtaatattt taaccgctag atgacgagca aacgcatgga
      841 gcgacaaaat gaataaagaa caatctgctg atgaagtctc gttggatctg attcgtgtaa
      901 aaaatatgct taatagcacc atttctatga gttacccgga tgttgtaatt gcatgtatag
      961 aacatcaggt gtctctggaa gcattcaggg caattgaggc agcgttggtg aagcacgata
     1021 agaattcgaa ggattattcc ctggtggttg actgagcacc ataactgcta atcattcaaa
     1081 ctatttcacc tgtgacagag tcaatatcgc attctgtcac tgtcaggcta atacagagct
     1141 gcaattcaac tactgcaatg tcctcgtaat taggtgaatt tacaatatcg tcctgttcgg
     1201 atgccggttg cattgctgaa gatgaggcat ttatggttcg catattttcc cctcatgctc
     1261 gtcagtcctg tgcgtaggaa gaaacaggac actcacacta atttgtgtgg gcatgctgtg
     1321 atgtccttct gaattattcc tatgccatta tgtaaagcgc tgtatcagat gctcatcacg
     1381 gctgtcaggc tgtcaggctg tcgggtcctc ccggtggggg cccctgccac ggggcgggag
     1441 cgtcgcggaa aaaggctagt ttttgcattt ccatggcggc ggcagcatgt ttggtaattt
     1501 attgataatt aaaagttatt tctcttttca cctgtacaat atttctttct ccctgtcatt
     1561 agaccagttt gcaattaatt gaaatatata aataaacatg attttcacct gccagatgga
     1621 gttgcttatg tcaaatgtga gcgggatcgg tgatgcttat tactggagtg tttttaaaat
     1681 cgctgaggcc tttgggcttc accgggacac agtaaaaaaa cggctcctcg cggctaacac
     1741 tcctgtggct gcgactgtca gggggaaccc cgtttacgcc ctgcagcatg tcgggccagc
     1801 cctgtttagt gtgaagcatg aggcagcaga ctctgttcat gatccatccc gtatggagcc
     1861 gaaagagaga aaggactggt accaatctga aaatgaaagg atcaagctgg aaaaggagca
     1921 gcgaaaactc atccccgttg atgaagtagt catcgtctat tcgtccatga gaaaggctgt
     1981 cgtccaggtt ctggagacaa ttccggatgt tcttgaacgc gattgcgccc tgactcctca
     2041 ggccgtcggc gttgtacagc aggccattga tgacctgcga tacactcttc aggaaaaatc
     2101 ctacgaggct tgtgctgctg aattaattcc tgatgaggaa ggagagagtc tctaggagga
     2161 ataatgggtt tttcatcagc ccgaaatttg ggaagggaca tatcggcagg attttcccca
     2221 ccacgtcgca tgccgatttc ggaggctgtt aaaaaattca tgcgtgttcc caagggggct
     2281 ggtaactcgg tgccatggga tcctgaactg acaccctaca tcattgagcc catgaactgc
     2341 ctggcatcgc gtgaatacga tgcggtgatt tttgttggtc ctgcgcgaac agggaagacc
     2401 attggtctga tcgatggatg gattgtctat accatcgttt gcgatccttc ggacatgctc
     2461 gttgtgcaga tgaccgaaga taaggcccgc gagcattcta aaaagcgcct cgacagaacg
     2521 ttcagaagca gtgcggcggt aaagaaaaga atgagtccac gtcgtaacga caataatgtc
     2581 catgataaga cgttcaggga tggctcgttc cttaaaattg gttggccctc ggtcaacatt
     2641 atgtcgtcgt cggattaccg gtttgtcgcc ttaaccgatt acgaccgttt tccggagaat
     2701 atcgacagcg agggtgatgg tttctccctg gcctcaaaac gtaccaccac atttatgtcc
     2761 gctgggatga ctctggtgga gagctcgccg ggacgtgaca tctgcgacag caaatggcga
     2821 cgtaagtcgc ctcatgaagc gccaccgacg actggtattc tttcccttta caatcgtggt
     2881 gaccgccgcc gctggtactg gtcatgtccg cactgcggtg aatattttca gccagccatg
     2941 gatgccatga ccggctaccg taatgaaccg gatcccttta aagccagtga ggcggcgtat
     3001 ctactttgcc cgcactgcag cggcattatc actgcggaga aaaagcgtga gctcaatagt
     3061 gcaggagtct ggttgcgtga aggtcaggtc attgatcgta acggcaacgt ttccggtgaa
     3121 ccgcgccgct cccgtatcgc cagtttctgg atggaagggc cagctgctgc gtatcagacc
     3181 tgggcgcaac tggtttacaa attactgact gcagaacagg agtatgaagc gacaggaagc
     3241 gaagaaacac tcagggcggt tatcaacacc gactggggat tgccttatct tcctcgtgcc
     3301 agcatggagc aacgaaaaag tgaactgctt gagcagcggg cagagccagt tccttcccgc
     3361 agtgtgccgg atggcgttaa tttccttgtg gcgacagtgg atgtgcaggc gggacgtcat
     3421 cgccgttttg tggttcaggt aacgggctat ggcagtcgtg gcgaacgctg gattattgat
     3481 cgttacaaca tcacgcagtc attgcgcagt gactgcgacg gggagagcca gcgaattgat
     3541 ccggccagct atccggaaga ctgggatgtc ctgctgacgg atgtttttca taaaagctgg
     3601 ccgctggcct ccgatccttc tcaacaaatg cgactgatgg caatggcggt ggactccggc
     3661 ggtgaagacg gggtcactga taatgcctat aaattctggc gtcgttgccg tcgtgatggc
     3721 cttggtaaac gtatttacct gtttaagggt gacagcatcc ggcgcgcaaa actgatcagc
     3781 cgtacattcc ctgataacac cggacgaacg ggccgccggg cgcaggccgc aggtgatgtg
     3841 ccgctctggc ttcttcagac ggatgccctg aaagaccggg tgaataacgc gttatggcgt
     3901 gactcgccag gtccaggcta tgtgcatttc cctgactggc tggggagctg gttttacgac
     3961 gaactgacgt atgaagagcg gagcagtgac gggaaatgga gtaagccggg tcgcggtgcc
     4021 aacgaagctt ttgacctgat ggtgtatgcc gaggctctgg tcattctgca tggatacgaa
     4081 aagatccgct ggccggatgc accggagtgg gcgagccggg agacctggct ggagtgtgtc
     4141 caggacagta ccgaaccgtc atcctcaccg gaaccggtat ccacgcctgt taaaaaacaa
     4201 aaacggaaga aaacagtaac tgacgatgtt aacccctggc tgacttccgg aggatggtta
     4261 tgaaccagaa tgatatcgaa gccatgattc agcgttatac ggaagctgaa atggcggtgc
     4321 tggacggaaa atccgtcacc tttaatggtc agcagatgac catggaaaac ttatctgaga
     4381 tccggcaggg acggcaggag tgggagcgcc gccttgcggc tctgattaca cgacgacggg
     4441 ggcatcccgg gtaccggctg gcgaggttct gatggcaatt cttgatgatg tgattggcgt
     4501 tttttcacca ggatggaaag cggcaaggct gcgttcccgt gcggtgatcc aggcttatga
     4561 ggccgtaaaa acgacgcgga cacacaaagc ccgacgggag aaccgaactg ccgaccagtt
     4621 aagccagtac ggggccgtgt cgttacgtga gcaggcccgt taccttgata acaaccacga
     4681 tctggttatt ggtgtatttg acaagctgga agaacgggtg gtggggaaaa acgggattat
     4741 tgtcgagcca catccggtat tacgcaatgg ggccattgcc cgtgacctgg ctgcggagat
     4801 tcgcacccga tggagtgaat ggtctgtcag cccggaagtc accgggcagt ttacccgtcc
     4861 gatgctggaa cgtctgatgc ttcgtacctg gctgcgcgat ggtgaggtgt ttgcccagat
     4921 ggtttccggg cgcataaaca gcctggcgcc ttctgccggt gttcatttct ggctggaggc
     4981 gctcgagccg gactttattc ccatgactag tgatgagagc aacaggctga atcagggcgt
     5041 gtttgttgat gactgggggc gtcccgaaaa atatctggtg tataaaagcc gtcccgtatc
     5101 cggacggcag atggaaacca aagaagtgga tgcagagcga atgctgcatc ttaaatttgt
     5161 tcgccgtctg caccagatgc gcgggacgtc tttgttgtcc ggtgtgctga tccgcctcag
     5221 tgccctgaaa gaatatgaag attctgagct gactgcagca aggatcgccg ctgctctggg
     5281 gatgtacatc cggaaaggcg acgggcagag ctatgaaccg gatggtaatg gcagaaagga
     5341 gaatgaacgt gagcttacca ttcagccggg cattatttac gacgatctga aacccggcga
     5401 agaaatcgga atggtgaagt cggatcgccc caatcctaac cttgaaactt ttcgtaatgg
     5461 tcagttgcgt gccgtggcgg cgggcagtcg tctgagtttt tccagtacag cgcgcaacta
     5521 taacggcact tacagcgccc agcgtcagga gctggttgaa tccactgatg gctacctgat
     5581 cctgcaggac tggtttattg gtgccgtcac ccgcccgatg tatcgtgcat ggctgaaaca
     5641 ggctgtggca tccggtgtta tcaggctacc ccgcgatctt gaccgttctt cactgtatac
     5701 cgcggtgtat tccggaccag tgatgccgtg gattgaccct gttaaggagg ctgaggcctg
     5761 gaaaatccag attcgtggtg gagcggcgac agaatcagac tgggtacgtg ctggtggtcg
     5821 taatccggat gatgtcaaac gtcggcgcaa ggccgaaatt gatgaaaacc gcaagctgga
     5881 tctggtattt gataccgatc cggccagtga taaaggaggc agtagtgccg caacgaaacg
     5941 acaggagccg cagcacaccg acgaccagtc cgaagaataa ttcctggttc aggatgcagg
     6001 ctggtcacca gagtgacgcg gatatttata tttatgacga gattggtttc tggggtgtta
     6061 cagcgaagca gtttatcagt gatctgaatg cactgggcga tatcacccac attaatctcc
     6121 atattaattc accgggtggc gatgtctttg aaggcatcgc catttttaat gcgctgaaaa
     6181 cacatggtgc gtccattacc gtttatgtcg acggtgtggc ggcgtcaatg gcgtcggtca
     6241 ttgcgatggt aggaaacccg gtcattatgc cggaaaactc cttcatgatg attcataaac
     6301 catttggctt tacgggcggt gatgcggagg acatgcgcac ctatgccgac ctgctcgata
     6361 aagttgaggc ggttctgtta cccgcttatg cacagaaaac cgggaaaacc accgatgaaa
     6421 ttgctgccat gctggcggat gagacctgga tgtccggtgc cgaatgtctg gcacagggat
     6481 ttgctgatca ggtgacacca gccgttaagg caatggcatg tattcagtca aaacgtacag
     6541 aggaatttaa aaagatgccg gaatccattc gaaatatgat tactccgcca cgcaacagtg
     6601 ctccacgcgt acaggatgat ggacctgcag cctcccggac gccagtgcag gcagcagcac
     6661 ccgtggtgga tgaaaacagc atccgtgcgc aggtcctggc agagcaaaaa gcgcgtgtaa
     6721 acggtattaa tgatctgttt gccatgtttg gcgggcgtta tcagtcgttg caggctcagt
     6781 gtcttgccga tcctgaatgt tcgctggagc aggcccgcga aaagctgttg aacgagatgg
     6841 ggcgcgagtc cacgccatcc aataaaaata ccccggctca tatttatgcc ggtaacggta
     6901 attttgtggg ggacgggatc cgccaggcgc tgatggcgcg cgccgggttt gaaaaaactg
     6961 aacgtgataa tgtctacaac gggatgaccc tgcgtgaata tgcccgtatg tcactgactg
     7021 aacgtggtat tggtgtttcc agttataacc cgatgcagat ggtcggtgcg gcgttcacac
     7081 acagtacgtc tgacttcggt aatattctgc tggatgttgc gaacaaagcc attctgcagg
     7141 gctgggaaga tgcccctgaa acctatgaac agtggacgcg gaaaggtcag ttgtctgatt
     7201 ttaaaattgc ccatcgtgtg ggtatggggg gcttcagtgc tctgcgtcag gtgcgtgaag
     7261 gggcagaata taaatacgtc accaccggag ataaacaggc cactattgca ctggcgacct
     7321 atggcgagct gttcagtatc acccgtcagg ccattatcaa tgatgatctg aatatgctga
     7381 ccgatgtccc gatgaagctg ggccgtgcgg cgaaatccac tattgccgat ctggtttatg
     7441 ccattctgac gtctaacccg aaaatctcca cagataatgt aagtctgttc gataaagcga
     7501 aacatgcaaa cgtactggag agcgcggcaa tggacgtggc atcgctggat aaagcccgcc
     7561 agttgatgcg cgttcagaaa gagggtgagc gtcatctgaa tattcgtcct gcgttcgtac
     7621 tggtaccgac ggcgatggag tctgttgcta accaggtcat tcgctcctca agtgtcaagg
     7681 gggctgacat taacgccggt attattaacc cggtgaaaga ttttgcgacc gttattgcag
     7741 agcctcgtct tgatgataac agccagacca ccttctacct ggctgcgtcc aaaggctccg
     7801 atacgattga agtggcttat ctcaacggtg tggatacgcc atatattgat cagatggagg
     7861 gcttcagtgt ggatggcgtg acaacgaaag tgcgtattga cgctggtgtt gcgccagttg
     7921 atcaccgcgg cctggtgaaa tgtacggcgt aaacgtcgca gacaacaact ctgatggccc
     7981 gtaagggctt tttttgtacc tgaaatcagc ccctgaacgg ggctgtgcgg agacagttat
     8041 ggcaaagaat tttgtagaag aaggaaaaac ggtggcgatt gttgccagtg cagccatcag
     8101 cagcggagat ctggtgcagg tgggggatgt ttttgcggtg gcgctgaccg atattccaca
     8161 gggagaaaca ggcgacggca taaccgaagg tgtgtttatg ctgcctaagc tgaaaacgga
     8221 tgacatgaaa acgggtaaga aggtttatct gaagtcagga aaagttcagc tgactaacag
     8281 cggctctgag ccgctggtcg gggttgtctg ggcagatgcc ggaaccagtg cagaagaagt
     8341 gccggtaaaa ctcaatgtct gatccctttt cccggctggc agcgcgtatg gatgcgatca
     8401 cggtcagaaa gatgggaaag acagcctcga ttaatgatgt cgatatgatt gtgatcccgg
     8461 gagaaacact ggcagagctg aatgctctgt ccggacctgc ggtctctctg gtggtgtttt
     8521 cttcgggata ccgcccacgg cgcggggatc gcgttgttta tgacggacaa caatggacgg
     8581 tcacacggca tgaacgtttt aacggtaagc caatgatctt tattgagtaa agaggtgtgg
     8641 gatgaagggg cttgagaatg ccatccgcaa tctgaacagc cttgataccc gtatggtgcc
     8701 acaggccagc gcatgggcga taaaccgtgt ggcacagaaa gcggtctcgg ttgccacccg
     8761 gcaggttgcc gggaataccg ttgcgggaga taaccaggtg aaagggatcc ccctgaaact
     8821 ggtacgtcag cgtgtccggg tgtttaaagc cagtccgtca ggaaaaatga cggtcaggat
     8881 ccgcgttaac cggggcaatc tgcccgccat taagctgggg acagcccggg tcagactggc
     8941 ccggcgtggt ggaaaactgc agtaccgtgg cagtgtgctg aaggtgggta aatatctttt
     9001 ccgggatgcg tttattcagc aactggcgaa tggtcgctgg catgtgatgc gacgtattga
     9061 tggcaaaaat cgttacccca ttgatgtggt gaaaatcccg ctgtccggat cgctgacaca
     9121 ggcatttgaa gatgcccgcg accacatcat tgctgcggaa atgccgaaac agctggggta
     9181 tgcactaaaa caacaactga ggttatggct gacccgatga accgacatac acaaatccgc
     9241 caggccgtac tggcacgcct tcgggaacag tgtggagaca gcgccacgtt ttttgacggg
     9301 cttccggcat ttattgatgc gcaggaactg cctgccgtgg cggtgtggct gagtgatgct
     9361 cagtacaccg gaaaaatgac ggatgaagat gactggcagg ctgttctgca tattgccgtc
     9421 ttcatccggg cacaggcacc ggattcagag ctggatatgt ggatggagag caccattttc
     9481 cctgccctga atgatatacc ggcactttcc ggactcatcg acaccctgat cccactcggt
     9541 tttaactatc aacgtgataa tgagatggcc acctgggcga tggcggaaat cacgtaccag
     9601 atcacgtaca cgaattaagg aggtggtaat gaccacacca aatccactgg caaagacgaa
     9661 aggtgcgggg acgacgttct ggatgtatac cggcaacggc gatgcgtttg cgaacccttt
     9721 gtcggacact gactggctgc gtcttgcgat ggtgaaggat ctgcaacctg gcgaaatgac
     9781 cgctgatgca gaagatgaca cttatctcga tgatgaagat gcagactgga aaacgacaac
     9841 ccaggggcag aaatccgttg gtgatacttc ggcgacgctg gcctggcgtc cgggtgacag
     9901 cgggcagaaa aaactggttc agttgttcga ctccggtgaa gtctgcgcgt ttcgtatcaa
     9961 atatcccaac ggcactgttg atgttttccg tggctggctg agctcactgg gtaaaaccat
    10021 tgcctcaaaa gacgtgatga cccgcactgt gaaaatcagc ggtgtggggc gtccgtatct
    10081 ggcagaggaa ggcactgaaa ccgtgagcgt taccgggctg acagtatcac cggcgtctgc
    10141 cagtgtaaaa gtgggagcaa ccaccacgct gacctttaca gtaaaacctg acggagccag
    10201 tgacaaagcg atcagtgtgc attcgtcaga tccacagact gccacggtta ccctgaacgg
    10261 acttgtggcc acggtaaaag gcgtgaagca gggcagtgtc agcattgtgg gcatgaccgc
    10321 tgacgggaat tttgtggctg tgactgcggt gactgtcagc gcagcaggtt aacaggacga
    10381 tactcatcat ttgccccggt tattcggggc ttttttgcag gtggagaaca tgatgtttct
    10441 gaaacaggac acgtttaatt atgaaaaaca gtccgtggtg ctcagtgagc tgtccgggct
    10501 gcagagaatt gaatatctga cgtttgttca gcagcgaacg gcaaagtttg atgccgggga
    10561 gggagaactg ccggaggctg aacgacagat tgcttttctg cggatgggga tggatatcaa
    10621 tgcctggctg gtttcccgct cactgtggaa tgcggatcag tctaaggatg tagagacgct
    10681 ttgcgcatcc gttattacaa catggtcgta tgatgccctg ggtgcggggg cggagatggt
    10741 tctgtcgctg agcggtatgg gggccattga taatgccgtg gatgatgagc atgaggcgct
    10801 gacgccggaa aagtcctgac gcgggaaatg cagtttgtca tgcggcttgc ccgggagttc
    10861 cggcgggcag actggcggcg gatgctgtcg gaaatgtcgg ccactgagct tggtgagtgg
    10921 ggcgattatt tccggatgca gagcttcagt gatgtgtgga tggatgcgca gtttgcctcg
    10981 ctgaaggcat tgatcgtgag aatggtgtcc ggcagcagtg atgctgcggt ggctgatttc
    11041 agccttttac cggaagagaa cgggataccg gagcgaacgg acgaagaact gatgcatctt
    11101 ggggaaggta tttccggagg tgtgcgttat ggaccagata gccaacctgg tcattgattt
    11161 ggggattgat gcggcagagt ttaaaaatga aattccccgt atcaaaaacc ttctgaatgg
    11221 tgcagccagc gatgcagaac ggtcttctgc ccgtatgcag cgttttatgg agcgtcagac
    11281 tcaggccgcc cggcagacaa cgcaggcagc ttcttcggct gcaacagccg catccgtcca
    11341 tgcgcagacg gtggagaaga acgcacaggc tcatgaacgc atggcccgcg aggtggagaa
    11401 aacccgccag cgcatggagg cactgagcca gaaaatgcgc gaggaacagg cgcaggccat
    11461 ggctctggcg gaggctcagg ataaagcggc tgctgcgttt tatcgtcaga ttgacagtgt
    11521 gaaacaggcc agtgcggggc tgcaggaatt acagcgtatt cagcagcaga tccgacaggc
    11581 cagaaacagt ggcgggattg gtcagcagga ttatctggcg ctgatttctg aggttactgc
    11641 gaaaacccgt gttcttacac aggctgagga agaggctacc cgacagaaag tggcgtttat
    11701 ccgtcagctt aaagagcagg caacccgcct gaatctttca tcttctgagt tgcttcgtgc
    11761 taaggcagcc cagctggggg taagcagtgc tgcagaagtg tatatccgca aaatggagca
    11821 ggcaggaaaa gccacgcatt cgctgggtct gaaaagtgca gcagcccgcc aggagatagg
    11881 cgttctgata ggtgaactgg cccgcggcaa tttaggggcg ctgaggggat ccgggataac
    11941 gctggctaac cgtgccggat ggatagacac actgatgtca ccgaaaggca tgatgcttgg
    12001 cggggttatt ggcggtattg ccgcggccgt ctatggtctg ggtaaagcct ggtatgacgg
    12061 tcagaaggag ggggaagaat ttaaccgcca gttgtcgctg acggggcatt atgccggagt
    12121 cactgccggg cagctgtgga cgctcagtcg tgctatttcc gggaatggta tcacgcaaca
    12181 tgctgcagcc ggtgcgctgg ctcaggtggt ggggagtggt gcatttcgtg gaaacgatat
    12241 cggtatggtg gcgagagttg ccgcacagat ggagcgatcg gttggccagt cggtcagcga
    12301 taccataaat cagtttaagc ggctgaagga tgatcctgta aatgccgcga aggctctgga
    12361 caatgagctg cattttctta ctgccactca gcttgagcag atacgcgtcc ttggggagca
    12421 ggggcggtcc agtgatgctg cacggatagc catgtctgca ctggcagagg aaaccggtcg
    12481 gcgtactgcg gatattgata ataacctcaa tgcgcttggc agtacgctga agtatctgtc
    12541 tgatttgtgg agtcgtttct gggatgcggc catgaatatt ggtcgtgaag actcgctgga
    12601 tgaacagatt gccgctttac aggagaaagt gtcgcgggcg aaaagactcc cctggacggc
    12661 atcatcttct caggttgaat acgatcagca gcgtcttaac gatcttcagg agaaaaaacg
    12721 ccagaaggat ttgcaggatg caaaagagca ggcagagcgg aattatcagg agcaacagaa
    12781 acgccgtaat gctgaaaatg ctgcactgaa ccggatgaat gaaacggaag cagcacgaca
    12841 tcagcgtgaa attgcgcgta ttaatgccat gcagtacgcc gatcaggctg tcagggatgc
    12901 ggcgatacaa cgtgaaaatg aacgttacga gaaagccctg gcatccggta agaaaaaaac
    12961 acgcgaaacc cgtaatgatg aggccacccg gttattgctg cagtacagtc agcaacaggc
    13021 acaggtggaa ggacagattg ctgctgccag acagtcagca ggcattgcca cggaaaggat
    13081 gacagaagcg cataaacagc ttctggctct gcagcagcgc atcagcgacc tggacgggaa
    13141 aaaactgacg gcagatgaaa agagtgtgct ggcccgtaaa gatgaactga ttcaggcact
    13201 gacgctgctg gatgtaaaac agcaggagct tcagaaacag acggcactca acgagctgaa
    13261 gaaaaaaaca attcagctga ccagtcaact ggctgaagaa gagcgcgctc agcgtcagca
    13321 acatgacctg gatatcgcca cggtgggtat gggtgatcag cagcgacagc gatatcaggt
    13381 acaactgagt cttcgccaga aataccagca acagctggag cagttgaggc gggatagtga
    13441 gcagaaaggg acatataaca cggatgacta cagaaaggcc gagcaggcgc tgacggagag
    13501 cctgaaccga caactgaatg agaatcgccg ttactggcaa cagcttgaag ttgtgcaggg
    13561 taactggaaa aacggagtcc tgcgtgcatt tcaggatttt accgtggatg cagataatac
    13621 ggcagaaaca gcagaacagg tgttctcgtc agccttcagc aacatgggaa atggcctggc
    13681 aacttttgtc actaccggca aactcaattt caaatccttc acctcttctg tgctgtcaga
    13741 tatggcgaaa atcctggcgc aggcaaccat gatgaaatcg ataaaaggga ttggcagtgt
    13801 actgggattt gatctcagca gcctttccct gaatgccaat ggggggattt atcagtctgc
    13861 tgatttgagt cgttacagtg gcacggtggt taaccgtccg acgttttttg cttttgcaaa
    13921 aggcgcgggt gtgatggggg aagcgggacc tgaagccatt ctgccattgc gtcgtggtgc
    13981 tgacggtaag ctgggggttg tggcggatat tggggggtca ggtatggcga tgttttcccc
    14041 gcagtacaac atcgagatca ataacgatgg cacgaacggg cagataggtc cggctgccct
    14101 gaaggcggtt tatgacctcg ggaaaaaagc ggcagcggac tttatgcaac agcaggcccg
    14161 tgatggtggt cggttaagtg gagcatatcg gtaatggaga cgtttcactg gaaagtgcgc
    14221 ccggatatga atgtggtatc agagccgaaa gtggtgacag tgaagctggg cgatggttat
    14281 gaacagcgtc gtgcggcggg actgaataac cagttgtcga cttacagcgt gacgatacgt
    14341 gttcgtaaat gtgaccaccc atctttaaaa gcctttctgg aacggcacgg tggcgtccgt
    14401 gcatttcagt ggacgccacc ttatgactgg aaaccgatca gggtggtttg tcgtaaatgg
    14461 tcggcaagcg tgggggcgct gtgggtaacc ataacggcag attttgaaca ggtcgtggca
    14521 taggaggctc tgatgcagga tattccacag gaaacacatc atgagacgac acgcctcact
    14581 cagtcagccc aggtggtgct ctgggaaatc gatctgacag aggtcggtgg tgaacgttat
    14641 tttttctgta atgagcagaa cgaaaaaggt gagccggtta tctggcaggg gcggcagtat
    14701 caggtatacc ccattcaggg gacgggattt gaactgaacg gcaagggcag tgctgcccgt
    14761 ccgacactga cggtttctaa cctgtacggt atggtcaccg ggatggcgga agatctgcag
    14821 agtctggtcg gcggaacggt ggtccggcgt aaggtttacg cccgttttct ggatgcggtg
    14881 aacttcgtca acggaaacag tgacgccgat ccggagcagg aggtgatcag ccgctggcgc
    14941 attgagcagt gcagcgaact gagcgcggtg agtgcctctt ttgtactgtc cacgccgacg
    15001 gaaacggacg gcgctgtttt tccgggacgt atcatgctgg ccaacacctg cacctggacc
    15061 tatcgcggtg atgagtgcgg ttatcacggt ccggcagtcg cggatgaata tgaccagccg
    15121 acgtccgata tcacgaagga taaatgcagc aaatgcctga gcggctgtaa gtttcgcaat
    15181 aacgtcggca actttggcgg cttcctttcc attaacaaac tttcgcagta atcccatgac
    15241 agagacagaa tcagcgattc tggcgcacgc ccggcgatgt gcgccagcgg agtcgtgcgg
    15301 cttcgtggtg agagcgccgg agggggaaag atattttccc tgcgtgaata tttccggtga
    15361 gccggaggat tatttccgga tggctccgga ggactggctg caggcagaga tgcagggtga
    15421 gattgtggca ctggtccaca gtcatcccgg tggtctgccc tggctgagtg aggctgaccg
    15481 gcggctgcag gtgcagagtg atttgccgtg gtggctggtc tgccgggggg cgattcacaa
    15541 gttccgctgt gtgccgcatc tcaccgggcg gcgctttgag cacggggtga cggactgtta
    15601 cacgctgttc cgggatgctt atcatctggc ggggattgag atgccggatt ttcatcgcgg
    15661 ggatgactgg tggcgtaacg gtcagaatct ctatctggat aatctggagg ccacagggct
    15721 gtatcaggtg gcgttgtcat cagcacaacc gggcgatgtg ctgctgtgct gttttggttc
    15781 atcggtgccg aatcatgccg ccatttactg cggcgacggt gagctgctgc accatattcc
    15841 tgaacaactg agtaaacgag agaggtatac cgacaaatgg cagcgacgca cacactccct
    15901 ctggcgtcac cgggcatggc acgcatctgc ctttacgggg atttacaacg atttggccgc
    15961 cgcatcgacc ttcgagtgaa aacggggtct gaagccatcc gggcgctggc catgcagatc
    16021 ccggcgtttc gtcagaaact gagcgacggc tggtaccagg tacgcattgc cgggcgtgat
    16081 gcaggtgaaa ccgaattgtc tgcccgtctt aatgagccgc tggcaaatgg tgccgtgatc
    16141 cacatcgtgc cgcgtctggc gggagctaaa agtggcggtg tgtttcaggt ggtgctgggg
    16201 gcggcgctga ttgcggtggc atggtggaac cctgtgggct ggctgggtgc cgcggctgta
    16261 tcgggcatgt atgcggcagg ggccagtatg atcctgggcg gagtggcgca gatgctggca
    16321 ccgaaagcca ggacgcccac ggcagcaagt acagataacg gcaaacagaa cacctatttc
    16381 tcctcactgg ataacatggt tgcccagggc aatgttctgc ccgttctgta cggtgaaatg
    16441 cgcgtggggt cgcgggtggt ctctcaggag atcagcacgg cagacgaagg ggatggtggt
    16501 caggttgtag tgattggtcg ctgatgaaaa acgttttatg tgaaaccgcc tccgggcggt
    16561 tttgtcgttt atggagcgtt aggaatgggt aaaggcagca gtaaggggca tactccgcgc
    16621 gaagcgaagg acaacctgaa gtccacgcag ttgctgagtg tgatcgatgc catcagcgaa
    16681 gggccgattg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccagtg
    16741 ctggacactg aggggaatac caacatctcc ggcgtcacgg tggtgttccg ggccggtgag
    16801 caggagcaga caccgccgga gggatttgaa tcctccggat ccgagacggt gctggggacg
    16861 gaagtgaaat acgacacgcc gatcacccgg accatcacgt cggcaaacat cgaccgtctg
    16921 cgctttacct tcggtgtgca ggcactggtg gaaaccacct caaaggggga caggaatcca
    16981 tcggaagtcc gcctgctggt tcagatacaa cgtaacggtg gctgggtgac ggaaaaagac
    17041 atcaccatta aaggcaaaac cacctcgcag tacttggcct ctgtggtggt ggataacctg
    17101 ccgccgcgcc cgttcagtat ccggatgcgc aggatgacgc cggacagcac cacagaccag
    17161 ctgcagaaca aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
    17221 ccgaacacgg cactggtcgg cgtacaggtg gattcggagc agttcggcag ccagcaggtg
    17281 agccgtaatt atcatctgcg cgggcgtatt ctgcaggtgc cgtcgaatta taacccgcag
    17341 acgcgacaat acagcggtat ctgggacgga acgttaaaac cggcatacag caacaacatg
    17401 gcctggtgtc tgtgggatat gcttacccat ccgcgctacg gcatggggaa acgtcttggt
    17461 gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agaattgcga ccagtcagtg
    17521 ccggacggct ttggcggcac ggagccgcgc atcacctgta atgcgtacct gaccacacag
    17581 cgcaaggcgt gggacgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
    17641 aacgggcaga cgctgacgtt cgtgcagaac cgaccgtcgg ataaggcgtg gacctataac
    17701 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
    17761 aa
//
LOCUS       DAZFKM010000099        17589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFKM010000099 DAZFKM010000000
VERSION     DAZFKM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            96..1235
                     /gene="arnB"
                     /locus_tag="ACHKAY_003498"
     CDS             96..1235
                     /gene="arnB"
                     /locus_tag="ACHKAY_003498"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO5702111.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1239..2207
                     /gene="arnC"
                     /locus_tag="ACHKAY_003499"
     CDS             1239..2207
                     /gene="arnC"
                     /locus_tag="ACHKAY_003499"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO5702112.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2207..4189
                     /gene="arnA"
                     /locus_tag="ACHKAY_003500"
     CDS             2207..4189
                     /gene="arnA"
                     /locus_tag="ACHKAY_003500"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO5702113.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4186..5076
                     /gene="arnD"
                     /locus_tag="ACHKAY_003501"
     CDS             4186..5076
                     /gene="arnD"
                     /locus_tag="ACHKAY_003501"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO5702114.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5076..6728
                     /gene="arnT"
                     /locus_tag="ACHKAY_003502"
     CDS             5076..6728
                     /gene="arnT"
                     /locus_tag="ACHKAY_003502"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO5702115.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6725..7060
                     /gene="arnE"
                     /locus_tag="ACHKAY_003503"
     CDS             6725..7060
                     /gene="arnE"
                     /locus_tag="ACHKAY_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO5702116.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7060..7446
                     /gene="arnF"
                     /locus_tag="ACHKAY_003504"
     CDS             7060..7446
                     /gene="arnF"
                     /locus_tag="ACHKAY_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO5702117.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7440..7706)
                     /gene="pmrD"
                     /locus_tag="ACHKAY_003505"
     CDS             complement(7440..7706)
                     /gene="pmrD"
                     /locus_tag="ACHKAY_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO5702118.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7816..9171)
                     /gene="menE"
                     /locus_tag="ACHKAY_003506"
     CDS             complement(7816..9171)
                     /gene="menE"
                     /locus_tag="ACHKAY_003506"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO5702119.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9168..10130)
                     /gene="menC"
                     /locus_tag="ACHKAY_003507"
     CDS             complement(9168..10130)
                     /gene="menC"
                     /locus_tag="ACHKAY_003507"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO5702120.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10130..10987)
                     /gene="menB"
                     /locus_tag="ACHKAY_003508"
     CDS             complement(10130..10987)
                     /gene="menB"
                     /locus_tag="ACHKAY_003508"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO5702121.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11002..11760)
                     /gene="menH"
                     /locus_tag="ACHKAY_003509"
     CDS             complement(11002..11760)
                     /gene="menH"
                     /locus_tag="ACHKAY_003509"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO5702122.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11757..13427)
                     /gene="menD"
                     /locus_tag="ACHKAY_003510"
     CDS             complement(11757..13427)
                     /gene="menD"
                     /locus_tag="ACHKAY_003510"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO5702123.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13516..14811)
                     /gene="menF"
                     /locus_tag="ACHKAY_003511"
     CDS             complement(13516..14811)
                     /gene="menF"
                     /locus_tag="ACHKAY_003511"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO5702124.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14890..15195)
                     /gene="elaB"
                     /locus_tag="ACHKAY_003512"
     CDS             complement(14890..15195)
                     /gene="elaB"
                     /locus_tag="ACHKAY_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO5702125.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15250..15711)
                     /locus_tag="ACHKAY_003513"
     CDS             complement(15250..15711)
                     /locus_tag="ACHKAY_003513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5702126.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15776..16693
                     /gene="rbn"
                     /locus_tag="ACHKAY_003514"
     CDS             15776..16693
                     /gene="rbn"
                     /locus_tag="ACHKAY_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO5702127.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16881..>17589
                     /locus_tag="ACHKAY_003515"
     CDS             16881..>17589
                     /locus_tag="ACHKAY_003515"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO5702128.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWI"
BASE COUNT         4143 a         4738 c         4431 g         4277 t
ORIGIN      
        1 ctatccttaa aatttcgctc caaatggcaa aatatacaca acactcttta tagcaaatat
       61 aagtggacag gtattcaatg gcggaaggaa aagcaatgtc agaatttttg cctttttcgc
      121 gaccagcaat gggcgtggag gaactcgctg cagttaaaga ggttctcgaa tccggttgga
      181 tcacaaccgg tccgaagaat caggcgcttg agcaagcctt ttgccagttg acgggaaatc
      241 agcatgccat cgcggtcagt tcagccaccg ccggaatgca tatcacgcta atggcgttgg
      301 aaattggcaa gggcgatgaa gtgattacgc cttccctgac ctgggtttca accctcaata
      361 tgatttcctt gttgggtgca acgccggtaa tggtggatgt cgaccgcgat acgccgatgg
      421 tcacgcctga agcgatcgag tcagccataa cgccacgcac taaagccatc attccggtgc
      481 attatgccgg tgcgccagca gatattgacg ccattcgcgc cattggcgaa cgttacggca
      541 tcgcagttat cgaagatgct gcccatgccg tcggtacgta ttacaaaggg cgacatattg
      601 gcgcaaaagg taccactatt ttttcatttc atgccattaa aaatattacc tgtgcggaag
      661 gtggcctgat tgtaactgat aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc
      721 acggtctggg tgtcgatgcc tatgacagac aaacctgggg ccgtgcaccg caggctgaag
      781 tcttaacacc gggctataag tacaatctga ccgatattaa cgccgcaatt gccctgacac
      841 agttagcaaa attagagcac ctcaacaccc gtcggcgcga aattgcccag caatatcagc
      901 aagcactggc agctctcccc tttcagccat taagccttcc cgcctggccg cacgttcacg
      961 cctggcatct gtttattatt cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt
     1021 tgatggaagc gttaaaagaa agaggcattg gtaccgggtt acatttccgc gccgctcaca
     1081 cacaaaaata ttatcgcgag cgttttccca cgctgtcgtt accgaatacc gaatggaata
     1141 gcgaacgcat ctgttctttg ccgctgttcc cggatatgac taccgccgat gccgaccgtg
     1201 tcatcacagc ccttcagcaa ctcgcaggac aataagccat gtttgaaatc caccctgtta
     1261 agaaagtctc ggtggttatt cccgtttata acgagcagga aagcttaccg gaattaatca
     1321 ggcgcaccac cacagcctgt gaatcgttgg ggaaagagta tgagatcctg ctgattgatg
     1381 acggcagtag cgataattcc gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc
     1441 atattgtgtc tattttgctt aaccgcaatt acgggcaaca ttcagcgatt atggcgggat
     1501 tcagtcacgt taccggcgac ttaattatta cccttgatgc cgatctccag aatccgccag
     1561 aagaaatccc ccgcctggtg gcaaaagccg atgaaggtta cgacgtggta gggactgtac
     1621 gccagaaccg ccaggacagc tggtttcgta aaacagcttc gaagatgatt aaccggctta
     1681 ttcagcgcac caccggcaaa gcgatgggtg actacggttg tatgctgcgc gcctatcgcc
     1741 gtcatattgt cgatgcgatg ttgcactgcc atgaacgtag cacctttatc ccgattctgg
     1801 cgaatatctt cgcccgccgt gccattgaaa ttccagtaca tcattccgag cgtgagtttg
     1861 gtgaatccaa atacagtttt atgcgcctga ttaatttgat gtacgacctg gtgacctgcc
     1921 ttaccacaac gccgctacgt atgctgagtc tgctcggcag cattattgcg attggaggtt
     1981 ttagcattgc ggtgctgctg gtgattttac gcctgacctt cggaccacaa tgggcggcag
     2041 aaggcgtctt tatgctattt gccgtgctgt ttacttttat tggcgctcag tttatcggca
     2101 tgggattact cggtgaatat atcggcagga tctacaccga tgtccgcgcc cgcccccgct
     2161 attttgttca gcaagttatc cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt
     2221 ttttgcctac cacgatatgg gatgcctcgg tattgaagct ctgctggctg ccggttacga
     2281 aattagcgcc atttttaccc ataccgataa tcccggtgaa aaagcctttt atggttcggt
     2341 ggctcgtctg gcagcggaaa gaggcattcc ggtttatgcg ccggatgacg ttaatcatcc
     2401 gctgtgggtg gaacgcattg cccaactgtc gccagatgtg attttctctt tttattatcg
     2461 ccatcttatt tgcgacgaaa ttttgcagct cgctcccgca ggtgcattta atctgcatgg
     2521 ttcgctgtta ccaaaatatc gtggtcgcgc gccgctgaac tgggtgctgg tcaacggtga
     2581 aacggaaact ggcgttacat tgcaccgaat ggtgaaacgt gccgatgccg gggccattgt
     2641 ggcgcaactg cgcattgcca ttgcgccaga cgatatcgct attacgctgc atcataaatt
     2701 gtgccatgcc gcgcgccagc tactggaaca gacattaccc gccattaaac acggtaatat
     2761 tctggaaatc gcccagcgcg aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga
     2821 cagtttcctt gaatggcata aaccggcatc cgtactgcac aacatggtac gtgccgttgc
     2881 cgatccgtgg ccgggtgcct tcagctatgt tggcaatcag aaattcaccg tctggtcgtc
     2941 gcgtgttcat cctcatgcca gcaaagcaca gccggggagc gtgatttctg ttgcgccact
     3001 gctgattgcc tgtggcgatg gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat
     3061 tactatgcag ggctcgcaat tagcgcagac gctgggcctg gtgcaaggtt cacgcttgaa
     3121 tagccagcct gcctgcaccg cccgacgccg tacccgggta ctcatcctcg gggtgaatgg
     3181 ctttattggc aaccatctga cagaacgcct gctgcgcgaa gatcattatg aagtttacgg
     3241 tctggatatt ggcagtgatg cgataagccg ttttctgaat catccgcatt ttcactttgt
     3301 tgaaggcgat atcagtattc attccgaatg gattgagtat cacgtcaaaa aatgtgatgt
     3361 cgtcttgccg ctggtggcga tagccacgcc gattgaatat acccgcaacc cgctgcgcgt
     3421 atttgaactc gattttgaag agaatctgcg cattatccgc tactgcgtga agtaccgtaa
     3481 gcgaatcatc ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata aatacttcga
     3541 tgaggaccat tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga tttattcggt
     3601 atcaaaacaa ttacttgatc gggtgatctg ggcctatggc gaaaaagagg gtttacagtt
     3661 caccctcttc cgcccgttta actggatggg accgcgactg gataacctta atgcggcacg
     3721 aatcggcagc tcccgcgcta ttacgcaact cattctcaat ctggtagaag gttcaccgat
     3781 taagctgatt gatggcggaa aacaaaaacg ctgctttact gatattcgcg atggtatcga
     3841 ggcgttatac cgcattatcg aaaatgcggg aaatcgctgc gacggtgaaa ttatcaacat
     3901 tggcaatcct gagaacgaag cgagcattga ggaactgggc gagatgctgc tggcgagctt
     3961 cgaaaaacat ccgctgcgcc atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag
     4021 tagcagctac tacggcaaag gatatcagga cgtagagcat cgtaaaccga gcatccgcaa
     4081 tgcccaccgc tgcctggact gggagccgaa aattgatatg caggaaacca tcgacgaaac
     4141 gctggatttc ttcctgcgca ccgttgatct tacggataaa ccatcatgac caaagtaggc
     4201 ttacgcattg atgtcgatac ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa
     4261 atcttgagta agcataatat tcaggccagc atttttttca gcgtcggccc ggacaatatg
     4321 ggccgccatc tctggcgact ggtgaagcca cagtttttgt ggaagatgct gcgctcaaat
     4381 gcggcatcgc tttatggctg ggatatttta ctggcaggta cggcctggcc aggtaaagag
     4441 attggtcatg ccaatgccga tatcattcgt gaagcggcta aacatcacga agtcggcctg
     4501 cacgcctggg atcaccatgc ctggcaagcc cgtagcggta actgggatcg gcaaacaatg
     4561 atcgacgata ttgcacgcgg tcttcgcact ctggaagaga ttatcggtca accggtaacc
     4621 tgttctgccg ctgcgggctg gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc
     4681 catttgcgct acaacagcga ttgtcgtggg gccatgccgt tccgtccatt gctcgaatca
     4741 ggaaaccctg gcactgcgca aattccggtg accttaccca cctgggatga agtgattggt
     4801 cgggatgtga aagcagaaga ttttaacggt tggttactca accgcatcct gcgagataaa
     4861 ggcacgccgg tttataccat tcatgcagaa gttgaaggct gcgcttatca gcataatttt
     4921 gtggatctcc tcaaacgcgc agctcaggaa ggagtgacat tttgcccttt aagcgaactg
     4981 ttatcagaga cgttgccgct cggacaagtt gttcgcggaa atattgccgg acgtgaaggc
     5041 tggctgggtt gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt taccttatcg
     5101 gcctcttcgc gtttattgcc tgctattacc tgttaccgat cagcacgcgt ctgctctggc
     5161 aacccgatga aacgcgttat gcggaaatca gtcgagaaat gctggcatcc ggcgactgga
     5221 ttgtgcccca tctgttaggg ctacgttatt tcgaaaaacc cattgccgga tactggatta
     5281 acagcattgg gcaatggcta tttggcgcga ataactttgg tgtgcgggca ggcgttatct
     5341 ttgcgaccct gttaactgcc gcgctggtga cctggtttac tctgcgctta tggcgcgata
     5401 aacgtctggc tctactcgcc acagtaattt atctctcatt gtttattgtc tatgccatcg
     5461 gcacttatgc cgtgctcgat ccgtttattg cattctggct ggtggcggga atgtgcagct
     5521 tctggctggc aatgcaggca cagacgtgga aaggcaaaag cgcaggattt ttactgctgg
     5581 gaatcacctg cggcatgggg gtgatgacca aaggttttct cgcccttgcc gtgccggtat
     5641 taagcgtgct gccatgggta gcgacgcaaa aacgctggaa agatctcttt atttacggct
     5701 ggctggcggt tatcagttgc gtactgacgg ttctcccttg gggactggcg atagcgcagc
     5761 gggagcctga cttctggcac tattttttct gggttgagca tattcaacgc tttgcactgg
     5821 atgatgccca acatagagct ccgttctggt actacgtgcc ggtcatcatt gccggtagcc
     5881 tgccgtggct gggattactc cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt
     5941 ccgcaaccgt ctatttgttg agctggacga taatgccgct gctgtttttc tccgtcgcta
     6001 aaggtaaatt gcccacctat attctttcct gctttgcatc tctggcaatg ctgatggcgc
     6061 attacgcttt gctggcagca aaaaataatc ctctggcgct gcggattaat ggctggatta
     6121 acatcgcttt tggcgtcact ggcattattg ccacatttgt ggtctccccg tggggaccaa
     6181 tgaacacgcc ggtgtggcaa accttcgaga gctataaagt cttttgtgcc tggtcgattt
     6241 tttcgctatg ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag acctggcctt
     6301 ttgccgcgct ttgcccgctg gggctggcgt tgctggtagg attttcaatt cctgacagag
     6361 ttatggaagg aaaacatccg caattttttg tcgagatgac acaagaatca cttcagccaa
     6421 gccgctatat tcttactgat agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac
     6481 gcgatgacat catcatgtat cgccagacag gtgagttgaa atacggcctt aattatccgg
     6541 atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa ctggcttaat caacatcgtc
     6601 aggaggggat tattactctc gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg
     6661 ccattccgcc cgcagatgcc atcgatcgtc aggagcgtct ggtgctgatt cagtatcgtc
     6721 ccaaatgatc tggctaacat tagtctttgc cagcttgctt agcgttgccg ggcagttgtg
     6781 tcagaaacag gcaacctgct ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg
     6841 gctgggactg gcgctggctt gtcttggtct tgccatggtg ctctggctgc tggtcttgca
     6901 gaacgtaccg gtaggcattg cctacccgat gttaagtctg aattttgtct gggtgacgct
     6961 ggctgcagta aaactgtggc acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt
     7021 cattattggt ggcattgtga tcctcgggag tacggtgtaa tgggcctgat atggggatta
     7081 ttcagcgtca ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg
     7141 ccgccgatga cgcacttatg ggattttatc gcggctctgt tagcttttgg cctggatgcc
     7201 cggatactgt tactcgggct gctgggttat ctgctttccg ttttttgttg gtataaaacg
     7261 ctacataaac ttgcgctaag caaagcctat gccttgctca gtatgagtta tgtgctggta
     7321 tggattgctt caatggtact tcccggctgg gaagggactt tttcgctgaa agcactactg
     7381 ggagtagcct gtattatgag cgggttgatg ctgattttcc tgcccacgac aaaacaacgt
     7441 tactgagttt tccctgccgc tgtacactgc cgttcccact catcggggct ataactgctt
     7501 gccgagagga ctttcaccgt atgcagcttt tcacgattaa tacaataaag cgcattctgc
     7561 aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac tcttcacttc ggcgatcatt
     7621 tttatcgcgc cgccagcatc gcagagcata agtacatgtc tgttatcttg tttattgcag
     7681 cacgattttt tgaccagcca ttccattgca ttatcctgtt tgctaagagt tttcacatca
     7741 atttacagat taacagataa actctttatc aacggcaatg gaatggcaga aatgagaagt
     7801 gaaacggagg gttaattatt gctgacgttg cacccactct tttagcgcct gacgtgaaat
     7861 tttaataccg ccgtttttca gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa
     7921 acgagccagc ttatctttca cccattcgcc aagatcaacg gtttgctggt catactccac
     7981 caccgctacc ggccgatgac caaactcctt gtcggcaacg ggaacgataa acacctgcaa
     8041 aaccgcagga tgtgcagcaa taacacgctc gacttcttcc ggctgaatac cctctccgcc
     8101 actgaagaat agattgtcta aacgtccgac aatggtcagc ttgccattat gcatctcacc
     8161 gcgatcgcgc gtagcgtacc agccttcgtc attaaccagt gaaaccagtt gcccgttacg
     8221 ccagtaacct tctgccatac tggcagcccg cagccacact tcattattaa cgattttcac
     8281 ttcccgaccc ggcagcggcg aaccaacgtc tgccaggccg tcggcttctt tcgcacacac
     8341 cgtggaggca aactcggtca gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc
     8401 ctgttccgtc aactcgaccg ggatagccgc gccgccaagt aacaccgctt tcagggaaac
     8461 ggaactgcgg ttaaccagca aacgccagag ttgtgttggc accagtgaag catgagtaca
     8521 gcctgccagc atttgctcca atggctgttt atcacgcacc gtcatccgcg caccagcgta
     8581 taaccagcgc cacataattc cctgaccgga gacgtgaaac agcggtaaag agagcaacca
     8641 gtcatcgtga tcgccaaacg gaatcaacga taacacccct tcggcactgg caagatgggc
     8701 ctgataagta tggacagcgg ctttcggcaa cccggtagaa cccgaggtca acgtcattga
     8761 gcacagacgc gtcggctgcc acgtagcggc atgtgcgcct tcaacccgct gaatgtgcag
     8821 cgacgttaac gccggaaacg tgttttcccc atccggcacc agcgcaaatt gcagcgtcag
     8881 attgggcagc aattcttcaa gcaacggttg cggcagctga gggttcacgg gcaacacccg
     8941 cgccccgcat tgcagtaacg ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg
     9001 caacatcacg ccgctgccct caacaacgcc ctgcgccgca aagcctgagg ctaattcatc
     9061 gacgcgagca caaagctcgc gccagttaag ttgctcgtca ttaagacgta aggcgatggc
     9121 ttctccccgc acttgccgcc agtgacgcca cggccagtca gagaagatca taacaaccgc
     9181 tccagtgcat caacttccac gacaggcagc gtgctacccg gccagcgacg tacctgctgc
     9241 gcctgcatca gatccagcgt gtccagccct ggaatggtgt ccggcgttaa ccaggcggca
     9301 atccgcgcca gttgcgttaa gcctaagctc gattcaatgg aagaactgat caccgccgtc
     9361 agccccagcg cgtgcgccgc ctgtacctgc tcgcgtactt tctccagact gcccgtgagc
     9421 gtgggtttga taactaccgc gcgcacgccc tcttcagcca caaaggcaaa atccggctcg
     9481 cgcaagcttt catcccaggc aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca
     9541 tcgcgggttt tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca
     9601 tatttggcaa actgctggcc tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa
     9661 tgcagatccg gaattgcctc cagcaacaga ttaaccacca tgccgtcgcg cacggcttcg
     9721 tacaatccca ctttgacctt cgccactttc tcgcctggca tatcagcaag tttgaggatc
     9781 agatcgtcag gatcgccatt acacagtggt gccgcacggt aattggccgc ctgcgggagc
     9841 gtatctgcca gctccgccaa tgcacagctt acgccaaagg caacggaagg catctgcggt
     9901 agctcgcaat cgcctgccag ccagttattt acccaggcaa gcagcacgct ttgcgcctct
     9961 tcccaggttt cctggctgaa gcccggcagt ggggagatct ccccccaccc ttcgcgctcg
    10021 ccctcacgca ggcaaacgta cagcccgtcg cgggttttta accgcctgtc gcgcagaacc
    10081 acccccgcgt ccatggggat ctgccagcgg tatacctgcg cgctacgcat tacggattcc
    10141 gtttaaattt gctgaaatca ggctgacgtt tctggttgaa agcgttgcga ccttcctgac
    10201 cttcttccgt catgtagaac agcatggtgg cgttgcccgc cagctcctgc agccccgcct
    10261 gcccgtcaca gtcggcgttc agcgcagctt tcaggcagcg cagcgccatc gggctgtttt
    10321 gcagcatttc gcggcaccaa cggacggttt ctttttccag atccgccagc ggtaccacgg
    10381 tgttgacaag gcccatatcc agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga
    10441 tttctcgcgc ttttttctgc ccgacgatac gagccatgta ggaagcgccc cagccgccgt
    10501 cgaaggaacc gactttcggg ccagtctgac cgaagatggc attatctgct gcgatagtca
    10561 ggtcgcacat catgtgcaga acgtgaccac cgccgatgga gtagccagcc accatcgcga
    10621 caaccggttt cggacaggta cggatctgac gctggaagtc cagcacgttc aagtgatgta
    10681 cgccggaatc atctttatag ccgccgtaat caccacgcac tttctggtca ccaccggagc
    10741 agaacgcttt atcgcctgcg ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg
    10801 catctgccag tgcctggatc atctctttta ccgtcagagg acggaaggca ttgcgcacct
    10861 gcggacgatt aatggtgatt tttgcgatac cgtcggtgga tttttcataa cgaatgtcct
    10921 cgaaaccttc ggagcagtcg tgccattcaa ccggtgcgta aagcattgct tcatcaggat
    10981 aaatcatatt gtgtccttta gtcagaaacg caagatctgc gccagacttg cgattacgcc
    11041 agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc agtcggcagc
    11101 cagttccgcc gccagggcgc ggaatttgct gtcacgttca ccacataaat aataaaacgc
    11161 aaatgtgcgg gcgctaaggt tagcacgtaa atcaggctgg acggcgagag aagtcgcctc
    11221 cagcatggcg gcaagggttg cgccattatt gttgctgcgc agcgccacca gctcccggcg
    11281 ttgatcgtca ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa ataccgccgt
    11341 taacggttct gtgcaaaaac gctgcgccca ttggcgatcg gaacgctgac gttccgcacg
    11401 ttgttcagca ttttgcagcc ccggatgccc gccttcgaca ataaccccac aaagccccgc
    11461 cagcccctgg caagccgcca tcatcgccac ccgtccgcca agcgagtacc ccaccagcca
    11521 gaagtcaagg atgttgtaac taaccaaggt tttacgcagt aagtcggtga catcatcaaa
    11581 tccatcgacg ctaatcgccg ccgaaccacc atgacctggg agatcaacat acaaccgtga
    11641 gtagtcggca aacgcctcgc ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg
    11701 caaaaacacc agccagggta aacctggttt tccgtgtttt gcctgcgcgt gcaggatcat
    11761 aaatggctta cctgcgccag aagttgctgg agcgtttgcg caccatcggt gtcgttaacc
    11821 accatttcaa tcaccgtggt ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt
    11881 tcctgccagt tttgcggacg atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa
    11941 tggacgtttt gcggcatcag atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc
    12001 gagaaaattt gcccgccgtt gttgttcacc acaattaata ccagcggcgc agaaacctga
    12061 cgcaataacg ccagcgcgtt gagatcgtaa agtgcggaga gatcgcccac aatcgccagc
    12121 gtcggtttgc cgcttgcccg ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg
    12181 ctggcccctc ggttgctgta caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc
    12241 agacgtacca ccaggctgtt accaacaaac aattgcccct gttcaggcag atagtcgctg
    12301 atgcgatgcg ccagttgcgc ttcgccaaac gcatcacggc gggcaataac cgcctgcatt
    12361 gcctgttccg ccaggcgcgg gatttcaacg caccagggct ggcgtttttc tgccggatgc
    12421 agctccagcc agtcggcaat attggcaatt aagcgacgtc cgcgatggtg tgccggatca
    12481 agtcgccctt caatgtcatc aacaatccag tactcttcgg gttcacagct tgcctgccat
    12541 tgcaggagcc gtttccccgt caggctgctt cccagttgca ccacaatttg cgcctgctgc
    12601 agctcgctgg tcgctttggc attgcctaac caaagatcgg cacacggcag cggctgcccg
    12661 gtttgtgaca gcacatcgcc aatcagcggc cagccaagag tttgcgccca cagggcaact
    12721 tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag
    12781 aagaaccagt cgcgctgttt ttcactttcc agacgaggcg cttcacgcag ccacggtttg
    12841 tcgtcctgcc accagtcacc cagacgctgt tgccagctaa gcccggtatc gtccatttcg
    12901 ccatacagcg gttcagcaaa cgggcagttg atatggactc cccccgcatg aagcgtaccg
    12961 agagcgtggt cgatggtaga aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc
    13021 aatgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctggcgaat tgcctgattc
    13081 gcgccgcagt caattagctc cggcgggcga tcggcggtta agagaatcag tttttctccg
    13141 gttaacccgg cttcaatcag tgccggatag agatttgcca ccgccgtgcc ggaggtcaca
    13201 atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccagatg ccccagtcca
    13261 cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc taacgttaac
    13321 ggtgtagaac gcgagcctgg ggcgatacag atgtgtctga cgccgtgacg cgttaatgct
    13381 tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag tttgtccggt
    13441 atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat gaatcggtaa
    13501 tgatgcgact cattactatt ccatttgtaa taaagtacgc agccctgccg ctttgttgtc
    13561 gatttcctgc cactcttgct cggggtcgga accacggaca atgcccgcgc cagcatatag
    13621 ccgcacgaca ttgccgctga tttttgctga acgcagggaa acgcagaatt cgctttgttg
    13681 taatgagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc
    13741 gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg gctgcaactg
    13801 atgtaaacag atcacatcat ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg
    13861 ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc
    13921 ctgtaatcgt tggcagatat cttccaccac cagcatgttc tcgcgctggt ttttatcatc
    13981 cgccatcagc cactctccta actgctgcgc ctgcttatca tcaggattat ttgctactgt
    14041 tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc gttccggtga
    14101 agagccaaga aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc aattcagatt
    14161 cagtcgacga ctggcagcca tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc
    14221 agttgcccga gcgagcacca ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag
    14281 ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa
    14341 atgtaaccca ggcaagggct tgatactcac cagtgtggcg ataaattctt ttgcctgaat
    14401 cgcatcgtgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc
    14461 acagcgtcgc cattccaggc ggggtaaaag taaattgccc tgcgacgggt caaaagcatt
    14521 cagcccccaa atgcgtaagt cggcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg
    14581 gtccaacgac gtaaaacggg taatcgcgcc caggacggct gcttcttcat caccattacg
    14641 ttgttgccag tagaattgcg ggtacgtttg ctgactggcc agccagctca aggcatcaaa
    14701 agcgtctttg agagggaaag gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc
    14761 ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca cgcggacctc
    14821 tccctgttaa aagcctcaca ttatacgggg tactacaaaa aaaatgcagt accccggtgt
    14881 agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg ccgcgcccac
    14941 accaattcct tgccagggtt tttcgtggac gtagtcatca gcacgataaa ctgcctgctt
    15001 cgcccgatag taataactgt ctgaagcctg gctaacccgt tttttcacat catccagcgc
    15061 tttttctgca cgcgctttca gctcaacata tttctgatcg gcgggatcgc cagaggagcg
    15121 gagcacctct tccagtgttt cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc
    15181 aaactgatta gacattctcg ttctccattt gcgtaaaacc tgtcactaac tatagacaag
    15241 ggttgttgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga taccatcttc
    15301 ttcatagacc tccgtcacag ggataaaacc gaaactctgg tagaagtttt gcagatgcgc
    15361 ctgcgccccg aggtaaacag gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt
    15421 actcatcagc tgctgcccca ctttttcgcc acgcaacgct tcactgacaa tcacccgacc
    15481 tataacgacc ggctcaagat catcatcact tttcagaatc ctcgcatatg ccaccagttc
    15541 atcatttttc caaccgagaa tatggcgatt atcccccgtc aaatcatcac catcgatatc
    15601 ctgataaggg caattctgct cgacaacaaa caccgcgcaa cgcagttgta ataaggcata
    15661 aagctgggaa accgataatt cagagtgatg cagatcttgc cattcaatca taatgctctc
    15721 cttttagatg tatgccttat actagccgga atattttttg aaacgtgatg aactcatgga
    15781 attaattttt ttaggtactt cggccggtgt gccaacccgc acgcgcaatg tcacggcaat
    15841 attgcttaac ctgcaacatc cgacccagag cggtctttgg ttgtttgact gcggtgaagg
    15901 cacccagcat cagctactgc ataccgcctt taaccctgga aaactggaca agatttttat
    15961 cagtcacctt catggcgatc atctttttgg tttacccggc ttgctgtgca gtcgttctat
    16021 gtcaggcatt atccaaccct taacgattta tggtccgcaa ggtatccgtg aatttgtgga
    16081 aaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg tcgaaattgg
    16141 cgctggcgaa attctcgatg atggcctgcg caaagtaacc gcttatccgc tggaacaccc
    16201 actggaatgt tatggctatc gtattgaaga acatgataaa ccgggtgcat taaatgccca
    16261 ggcattaaaa gctgctggcg tgccgcctgg cccactgttt caggaattaa aagcgggcaa
    16321 aacaatcacg ctggaagatg gaaggcagat taacggcgca gattacttag ctgctccagt
    16381 gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcgatgccg cacttgacct
    16441 ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca tggaagccaa
    16501 agccaatagt cgcggccata gctctacacg ccaggctgcg acactagccc gtgaggctgg
    16561 agtcggtaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag gttgtcagca
    16621 cctgttacgt gaatgcaggt caattttccc ggcgactgaa ctagcgaatg atttcaccgt
    16681 gtttaacgtt taactctgct tattattaaa aacagggcga aacttgccct gttatcgcaa
    16741 cccgcgctac atatatccac ccacacgggc atattgagcg atttttgata tttattcatc
    16801 tccttacgca ctatattcag agcaataagt tgcctctttt tatcttcatt cagcacaacc
    16861 tttgcaacta aaaggagccc atgatggtta cagttgtcag caattattgt caattatctc
    16921 aaacccaact cagtcagaca tttgcagaaa aatttactgt gaccgaggaa ttactgcagt
    16981 ctttaaaaaa aacagcgtta tccggagatg aagaaagtat tgagttactg cataatattg
    17041 cgttaggtta tgatgaattt ggaaaaaaag ctgaagatat tctttaccat attgttagaa
    17101 acccaacaaa tgagacccta tcgattatcc gacttattaa aaatgcctgt ttaaaattat
    17161 ataatttggc acataccgca accaactccc accttaaacc aactggtccg gataattcag
    17221 atgttctcct gttcaaaaag ctattctccc cttcgaaatt aatgacaatt atcggtgatg
    17281 aaattcctct tatatctgaa aaacagtcgc tttcaaaggt gcttttaaat gatgagaata
    17341 atgaactgag tgatgataca aacttctggg ataaaaatcg tcaattaacc acagatgaaa
    17401 tagcttgcta tcttcagaag atcgccgcta atgcaaaaaa tactcaagtc aattatccta
    17461 ctggtctcta cgtcccctac tccaccagaa ctcacctgga agacgctctc aatgaaaata
    17521 ttaagagcga tccatcatgg ccgaatgaag tccagttatt ccacataaat actggcggac
    17581 actggatat
//
LOCUS       DAZFKM010000100        17584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFKM010000100 DAZFKM010000000
VERSION     DAZFKM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            197..451
                     /gene="bssS"
                     /locus_tag="ACHKAY_003516"
     CDS             197..451
                     /gene="bssS"
                     /locus_tag="ACHKAY_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO5702129.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            566..1684
                     /gene="solA"
                     /locus_tag="ACHKAY_003517"
     CDS             566..1684
                     /gene="solA"
                     /locus_tag="ACHKAY_003517"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO5702130.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1732..1845
                     /locus_tag="ACHKAY_003518"
     CDS             1732..1845
                     /locus_tag="ACHKAY_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO5702131.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2106..2672
                     /gene="yceJ"
                     /locus_tag="ACHKAY_003519"
     CDS             2106..2672
                     /gene="yceJ"
                     /locus_tag="ACHKAY_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO5702132.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2676..3251
                     /gene="yceI"
                     /locus_tag="ACHKAY_003520"
     CDS             2676..3251
                     /gene="yceI"
                     /locus_tag="ACHKAY_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO5702133.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3293..4345)
                     /gene="trhO"
                     /locus_tag="ACHKAY_003521"
     CDS             complement(3293..4345)
                     /gene="trhO"
                     /locus_tag="ACHKAY_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO5702134.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4570..5490
                     /gene="lpxL"
                     /locus_tag="ACHKAY_003522"
     CDS             4570..5490
                     /gene="lpxL"
                     /locus_tag="ACHKAY_003522"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO5702135.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5662..6888
                     /gene="mdtG"
                     /locus_tag="ACHKAY_003523"
     CDS             5662..6888
                     /gene="mdtG"
                     /locus_tag="ACHKAY_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO5702136.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6971..7345
                     /gene="msyB"
                     /locus_tag="ACHKAY_003524"
     CDS             6971..7345
                     /gene="msyB"
                     /locus_tag="ACHKAY_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5702137.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7346..7573)
                     /gene="yceK"
                     /locus_tag="ACHKAY_003525"
     CDS             complement(7346..7573)
                     /gene="yceK"
                     /locus_tag="ACHKAY_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO5702138.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7746..10289)
                     /gene="mdoH"
                     /locus_tag="ACHKAY_003526"
     CDS             complement(7746..10289)
                     /gene="mdoH"
                     /locus_tag="ACHKAY_003526"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO5702139.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10282..11817)
                     /gene="mdoG"
                     /locus_tag="ACHKAY_003527"
     CDS             complement(10282..11817)
                     /gene="mdoG"
                     /locus_tag="ACHKAY_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO5702140.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12211..13368
                     /gene="mdoC"
                     /locus_tag="ACHKAY_003528"
     CDS             12211..13368
                     /gene="mdoC"
                     /locus_tag="ACHKAY_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO5702141.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13376..14857)
                     /gene="clsC"
                     /locus_tag="ACHKAY_003529"
     CDS             complement(13376..14857)
                     /gene="clsC"
                     /locus_tag="ACHKAY_003529"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO5702142.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14799..15332)
                     /gene="ymdB"
                     /locus_tag="ACHKAY_003530"
     CDS             complement(14799..15332)
                     /gene="ymdB"
                     /locus_tag="ACHKAY_003530"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO5702143.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15427..15738)
                     /gene="ymdA"
                     /locus_tag="ACHKAY_003531"
     CDS             complement(15427..15738)
                     /gene="ymdA"
                     /locus_tag="ACHKAY_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO5702144.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15859..16191)
                     /gene="csgC"
                     /locus_tag="ACHKAY_003532"
     CDS             complement(15859..16191)
                     /gene="csgC"
                     /locus_tag="ACHKAY_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO5702145.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16250..16705)
                     /gene="csgA"
                     /locus_tag="ACHKAY_003533"
     CDS             complement(16250..16705)
                     /gene="csgA"
                     /locus_tag="ACHKAY_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO5702146.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16746..17201)
                     /gene="csgB"
                     /locus_tag="ACHKAY_003534"
     CDS             complement(16746..17201)
                     /gene="csgB"
                     /locus_tag="ACHKAY_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO5702147.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4407 a         4467 c         4159 g         4551 t
ORIGIN      
        1 ttgcacaata tgctaaaaaa atatgaatga catgctgttt tattgttcat ataaatctta
       61 aaagcagaaa tttatctgtt ccccccttta aaaattcggt gaataccctt acttattggt
      121 atactgaagt tgcttcagat aaaaacgcat tgaacctcga ataacgttgt ctagtaacac
      181 gaattagggg gccatgatgg aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg
      241 gtgggacatc agcaccgttg atagctatga tgcgctgatg ttgcgtttgc actaccagac
      301 cccaaataag tccgagctgg aagggactga agttggtcag acgctctggt taaccactga
      361 tgttgccaga caatttattt cgatattaga agcaggaatc gccaaaattg aatccggtga
      421 tttcccggta aacgagtatc ggcgtcatta acatgacatg accatccacc caaaaggcac
      481 ctcaccggtg cctttaccat ttctgggttg tatatctgca ttcagttaat tactctgctt
      541 aaaccatgag agcagagaga aaaaaatgaa atacgatctc atcattattg gcagcggttc
      601 cgtaggcgct gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc taatgaccga
      661 cgcccatatg ccaccgcatc aacacggcag ccaccatggc gatacgcgat taattcgcca
      721 tgcttatggt gaaggcgaaa agtatgtccc gctggtcctc cgcgcgcaaa cgctgtggga
      781 tgaactttcc cgccacaacg aagatgatcc catttttgta cgctctggtg tcattaacct
      841 cggcccggct gactccgcat ttctcgccaa cgtcgcccac agcgccgaac agtggcaact
      901 caacgttgaa aagctcgatg cgcaagggat tatggcccgc tggccagaaa tacgcgtccc
      961 ggacaactac atcggcttat ttgagactga ttccggtttt ttgcgcagcg aactggcgat
     1021 taaaacctgg atccaactgg cgaaggaagc gggctgtgcg caactgttca actgcccggt
     1081 caccgtaatt cgtcatgacg atgatggcgt aactattgaa acggttgacg gtgagtatca
     1141 ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc
     1201 tgtccagccc gtacgtaaag tatttgcctg gtatcaggcc gatggccgct atagcgtgaa
     1261 gaataaattc ccggcgttta ccggtgaact gcccaatggc gatcaatatt atggttttcc
     1321 ggcagaaaac gacgcgttga agattggcaa acataacgga ggccaggtta tccattcagc
     1381 ggatgaacgt gttccgtttg cggaagtggt cagcgatggt tcggaagcct tcccgttctt
     1441 gcgcaatgta ttgccgggta tcggttgctg cctgtacggc gctgcctgca cctatgataa
     1501 ttcgcctgac gaagatttta ttatcgatac cctacccggc cacgataata cactgctcat
     1561 taccggcctg agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga
     1621 ttttgcgcaa gacaaaaaaa gcgattttga tttgacgcca ttcaggcttt cccgcttcca
     1681 ataatcatca ttacggcctc cagaatgagg ccgtttttac aggagtattt aatgcgtcgg
     1741 ctgttgcact atctcatcaa taatattcgc gagcatctga tgctttatct ttttctctgg
     1801 ggattgttgg caattatgga tctgatctat gtgttttatt tttagttttc cgcactgaca
     1861 taaactgaca aatatttaat ctccctttca tttggcgtga aaaattgaat gagattattc
     1921 ctgcttctac agcactcacc agacatctct gatgctgaac ccactaaact tatgaagatt
     1981 ctttcattat taattcaaaa ttattgaaat aaaaataaat atcattcaat ttgttatttt
     2041 tgtgttgcaa ttcattctcc gacacgccat tttctttctc tcgcaaattc gtattggatg
     2101 cacttatgtc attcacaaat acccctgaac gctatggtgt tatatctgcg gccttccatt
     2161 ggctaagcgc aattattgtc tacggcatgt ttgctttagg tctgtggatg gtcacgctca
     2221 gttattacga tggctggtat cacaaagcac ccgaactgca taaaagcatc ggtattttgt
     2281 taatgatggg gctggttatt cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc
     2341 tgccgagtta ttcgcccatg actcgtcttg ccgcaaaggc tggacatctc gcattgtacc
     2401 tattgctgtt cgccatcggc atcagcggct atctgatctc aactgccgat ggtaaaccga
     2461 tcagcgtttt tggctggttt gacgtccccg cgacccttgc cgacgctggc gcacaggcag
     2521 actttgccgg tgccctgcat ttttggctcg cgtggagcgt cgtagtactg tccgttatgc
     2581 acggatttat ggccctgaag catcatttca tcgataaaga cgacactctg aagcgcatgc
     2641 tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa
     2701 ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc cgccgattac aaaattgaca
     2761 aagaaggtca gcacgccttt gttaacttcc gcatccagca tcttggctat agctggttat
     2821 atggcacctt taaagatttc gacggtactt ttacctttga cgaaaaaaat ccggctgccg
     2881 ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac taatcacgcc gaacgcgata
     2941 aacatcttcg cagtgcagat ttcctcaata ccgcaaaata tccacaggca acattcacct
     3001 ccaccagcgt gaagaaagac ggtgacgaac tggatattac cggcgatctg acgctgaatg
     3061 gcgtaaccaa acccgtcacg ctggaagcga aattaattgg tcagggcgac gacccatggg
     3121 gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa gctcaaggac ttcaatatca
     3181 agacagattt aggtccagct tctcaggaag tggatctgat tatttcagtg gaaggcgtac
     3241 agcagaagta agcgtgaaat gttgccggat ggcacgcatc cggcaatgat atttattccg
     3301 ttggatcagg aatgcccagt gttgtattca gacgtccacg agacttatta aagatcttat
     3361 tgccattttc acgtcctgcc cgacggcgtc gctgttcttc tggcggtaac gcgctttctt
     3421 cgcagcaaat ctcactacaa caacctttgt atttttccgc acatactgga cactgaataa
     3481 acagcaggtg gcagccatca tttttacagt tggtatggct gtcgcacggc gcgccgcact
     3541 ggtggcaatg cgcgataatc tcatccgata tacgttcgcc catccgctcg tcaaaaacaa
     3601 aatttttgcc aataaaacgc accggcaagc cctgctcgcg cgccttacgg gcgtattcaa
     3661 taattccgcc ctcgatatgc cagactttgt tgaatccgtt atgtttcatc caggcactgg
     3721 ccttttcaca acgaatgccg ccggtgcagt acatgacgat ttttttatct ttatgtgcct
     3781 gcatcatctc gactgctttt ggcagctgct cacggaaggt atctgccgga atttcgagcg
     3841 cgttttcaaa gtgccccact tcatactcat agtggttacg catgtcgata aatagtgcat
     3901 cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag atactcgcca acattgctgg
     3961 catcaaagtg aggatcgtca ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca
     4021 gtacccagaa agatttcccg tcatcatcca acgcgatatt caggcgtaaa ccctctaaag
     4081 ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga
     4141 tttgcgcgtt aatgccctca tgcgccagat acactcgccc aaaaacattc agcgcggtaa
     4201 acagctgata taaagcgtca cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa
     4261 acgaaatggt ggttcgcggt tcgctctcag ccaacatttt ggcttttagc gcgtcgttgg
     4321 aaatgcggtt gtgtaacact ggcatggtgt acggttcctg cgagatggga aagtaaaaat
     4381 ccgcggcatg atatagcaat tatcgataat taacatccac acattttacg ctacatttgc
     4441 gcattaaaaa ttatttgtta tttacaagtg cggcaatttc gcccagtctt cagccacaaa
     4501 ttttggttgc gggcgaaaaa atgcgacaat acatacaatt gcccgaatag gttgaaaaac
     4561 aggattgata tgacgaatct acccaagttc tccaccgcac tgcttcatcc gcgttattgg
     4621 ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc aattgcccta cccggttatc
     4681 taccgcctcg gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa
     4741 attgtgcatc gcaacctgga actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa
     4801 atggtggtga agaatttcga atccgttggc atgggcctga tggaaaccgg catggcgtgg
     4861 ttctggccgg accgccgaat cgcccgctgg acggaagtga tcggcatgga acacattcgt
     4921 gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca tccattttct gacactggag
     4981 ctgggtgcgc ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg cccgaacgat
     5041 aatccactga ttgactggct acaaacctgg ggccgtttgc gctcaaataa atcgatgctc
     5101 gaccgcaaag atttaaaagg catgattaaa gccctgaaaa aaggcgaagt ggtctggtac
     5161 gcaccggatc atgattacgg cccgcgctca agcgttttcg tcccgttatt tgccgttgag
     5221 caggctgcga ccacgaccgg aacctggatg ctggcacgga tgtccggcgc atgtctggtg
     5281 cccttcgttc cacgccgtaa gccagatggc aaagggtatc aattgattat gctgccgcca
     5341 gagtgttctc cgccactgga tgatgccgaa actaccgccg cgtggatgaa caaagtggtc
     5401 gaaaaatgca tcatgatggc accagagcag tatatgtggt tacaccgtcg ctttaaaaca
     5461 cgcccggaag gcgttccttc acgctattaa atctcccctg ccggatgctt cagaatggca
     5521 tccggcatta ccacagcaaa tccccctgat ttagcgataa aagctctctg gattgcgccc
     5581 cctggaagtc gagcgcataa ttagtgtgct tatcttttct tcttatgttc accgcgcctg
     5641 gcgcaccaac agcggattgc tatgtcaccc tgtgaaaatg acacccctat aaactggaaa
     5701 cgaaacctga tcgtcgcctg gctaggctgt tttcttaccg gtgccgcctt cagtctggta
     5761 atgcccttct tacccctcta cgttgagcag cttggcgtta ccggtcactc cgccctgaat
     5821 atgtggtccg gtattgtctt cagcattaca tttttatttt cggccatcgc ctcaccgttt
     5881 tggggtggac tcgccgaccg taaaggccga aaactcatgc tattacgctc tgccctcggc
     5941 atgggcatcg tgatggtgtt gatgggactg gcacaaaata tctggcagtt tttgatccta
     6001 cgggcgcttc ttgggttact tggcggattt gtccccaacg ctaatgctct tatcgccaca
     6061 caagtaccgc gtaataaaag cggctgggcg ctgggtacgc tctccacagg cggcgttagt
     6121 ggtgcgttgc tcggcccaat ggctggcggc ctgctcgccg atagctacgg cttacgtccg
     6181 gtattcttta ttaccgccag tgtgctcata ctctgctttt tcgtcaccct gttctgcatc
     6241 agagaaaaat tccagccggt cagcaaaaaa gagatgctgc acatgcggga agtggtgaca
     6301 tcacttaaaa acccgaaact ggtactcagc ctgtttgtca ccacgttaat catccaggtg
     6361 gcgacaggct caattgcccc cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc
     6421 agtaacgtcg cctttatcag tggcatgatc gcctcggtgc caggcgtggc ggctctgcta
     6481 agcgcaccac gactcggcaa acttggcgat cgaatcggac ccgaaaagat cctgattaca
     6541 gcgctgatct tttctgtact gctgttgatc ccaatgtctt acgttcagac gccattgcaa
     6601 cttgggattt tacgtttttt gctcggtgcc gccgatggtg cactactccc cgccgtacag
     6661 acactgttgg tttacaactc gagcaaccag atcgccgggc gtatcttcag ctataaccaa
     6721 tcgtttcgtg atattggcaa cgttaccgga ccattgatgg gagcagcgat ttcagcgaac
     6781 tacggtttca gagcggtatt tctcgtcacc gctggcgtag tgttattcaa cgcagtctat
     6841 tcatggaaca gtctacgtcg tcgtcgaata ccccaggtat cgaactgatt tttcgccttt
     6901 catacttgca aaagcggaga atcagctatc cttttccctg aaacctcatc aactcaaagg
     6961 gagaatcgtg atgaccatgt acgcaacgct tgaagaagcc attgacgctg cacgcgaaga
     7021 atttcttgca gacaaccccg gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc
     7081 ccaaaaatac gttttgcagg acggcgacat catgtggcaa gttgagtttt ttgccgacga
     7141 aggggaagaa ggtgaatgtt tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga
     7201 tggcgactat gatgagatag agatacgcca ggagtggcag gaagagaata cattacatga
     7261 atgggacgag ggggaatttc agcttgagcc accgctggat accgaggaag gacgcgcagc
     7321 agctgatgag tgggatgaac gttaatcact catacgggcc atgatgaatg tcgatcggca
     7381 gcagtaaagt atcaaaaacc agagagaatg gcagatcaag gatcgtgaca taacgccagg
     7441 cggagtcacg cacatcccat tgcacaccgg gataatattg gttgccatgc ccctgccccg
     7501 gtatagtgcg actaataatg ctgccacagc cactcagcaa ggtcaccatg atgctcacca
     7561 caattaaacg catttgatac ctctttcgta aaaaaatacc ggcataacgc cggtattttt
     7621 attggctcgt tgtttttgcc ggatgcggcg tgaacgcctt atccgaccca caaaatcttg
     7681 caaaatcaat aaattgcagg aacgatgtag gcctgataag cgtagcgcat caggcaacta
     7741 cgtttttatt gcgaagccgc atccggttta cgcaatgcca gtggattgag ctttatccct
     7801 tcgtaataac tcacccatga agaatatctc tccggggaat tccagacgcg gaaatgcaga
     7861 cgggccatcg tcaccggatc gcttagcagc accaggcgac gatcgcgatt cagcttctct
     7921 ggcgtctcgt tcagcgcctg ttcaacgtgg cggtcacggg cgatttccag caccttactg
     7981 gcgcggtgac gcgcggtcgc cattgcggtt gccagagcgt taaatgacgg gttaaacact
     8041 gcgtgcataa agccatcatc aagggagcgt tgacgattca tctcaaggaa ccgatcggta
     8101 tcaaccagca cctgcggcgg cgaatactct tccgggatca ggaacagttt ccagcgtttg
     8161 gtgcgcagac caacggtggc acggctggaa atcaccgaaa caaacggtga caggatcaac
     8221 gagaagacaa tcggtgccag ccagaacagg aaacgcagat ccaaccacgc cataccaaca
     8281 gcccacacta accctaacag cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa
     8341 gtggagtcat catcacgctg cggtgaattc cacaccactt cccagccaag gaacgcgctg
     8401 acgacgaaga ccgtatggaa cagcatgcgt accggagcca gcagcacgga aaacagcact
     8461 tccagcagca acgataatgt aacgcgccag aagccgccgt attctttcgt tcctttgcac
     8521 cagataagca aaatgctcaa taacttcggc aggaacaaca gcaccatggt cgaagcaaaa
     8581 agtgcaatcg ccagctcagg acgccactgc ggccacaccg ggaacaactg ccgtggttgc
     8641 aggaagtatt gcggttcggt caacgcatgt actacctgca atgcagtaga gagcgcgagg
     8701 aacataaacc acagcggagc ggagagataa gacatcacgc ccgtcaggaa taccgcacgg
     8761 tgaaccgggt gcataccctt caccaggaac agacggaagt tcatcaggtt accgtggcac
     8821 cagcggcggt cacgttttag ctcatcaagc aagttaggcg gcaattcttc ataagaaccc
     8881 gggagatcgt aagcaatcca gaccccccaa cctgcacggc gcatcaacgc cgcttccacg
     8941 aagtcatgtg acaggattga accggcaaag gaaccttcgc ccggcagcgg agccagtgcg
     9001 cagtgctcga taaacggttt cacgcggata atcgcgttat gtccccagta gtgcgactcg
     9061 ccaagttgcc agaagtgcaa accggctgta aacagtggcc catacacgcg ggtcgcgaac
     9121 tgctgacagc gcgcatacag cgtatccata ccggacgctt tcggcgacga ctgaatgatc
     9181 ccggcgttcg ggttggcttc catcaggcgc accaggccgc ataaacaatc accggtcatt
     9241 accgagtcag catccagcac caccatgtag ctgtactggc tgccccagcg acggcagaag
     9301 tcatcgatat taccgctttt acgcttcacg cggcgacggc ggcggcgata gaaaatctga
     9361 ccttcgccac cgacttcagc gataagctcc atccaggctt tttgctctgc gacgcagata
     9421 tccgggttat aactgtcact aagaatgtag acatcaaagt gtttggcatt cccggtggct
     9481 tttactgatt cccacgttgc acgcaggcca gcaaaaacac ggttcacgtc ttcgttacag
     9541 ataggcatga tcaacgccgt gcgatgctcc gggtttaatg gttcatcgcc aactgttgac
     9601 gcagatatac tgtatttatc gcgaccaata agcagttgca ggaagcccat taacgccgtc
     9661 cagaatccgg cggacaccca acagaacagt accgcaaaga ggatcaggat accggtttgc
     9721 agcatataag gcagaagctg cataaaggaa acccacaaat cctgaccaac catatccata
     9781 ggattaatca gcgcccaacc ctgataagga agaatggtct tcatatacca ggtcgcgacg
     9841 acagtttgcg cgagcgtcag gatcaacaga atgtaacggc ggatggtacc gacggtacgc
     9901 cacttttgct cgctctcctg ctcttctttg gtcaaacgag ccagatagcg cggcgtgaca
     9961 tcgcgtccac gcaggcgatc ccagaaacgg cctaccgggt tggtacgcca cgggtcggga
    10021 aacatcgagg agcgttttgc ttctggcatc gccttcagct gatcgcgccc ttcgtcgtct
    10081 ttaattaact gtccatcagc aagtgaatct ggccaggctt gttccagacg cgcctttacc
    10141 gagccttgcg gggaatcatc ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga
    10201 tgaacggcgc ggatatcagt cttcggcaat gccgctttct cgcttgcggc gatgggcatt
    10261 gcgtcaatgt actcagttgt cttattcatt ggcaggtaac tggtagctcc aggtttcact
    10321 caacgtctga tcggcattca ccagcgcagc acgcatttca gtggttttct tggcatcttt
    10381 cactttcaca cgcatcacca gacgccagcc tttggtaacc gggttatagc gcaccgtgct
    10441 ttcaactatc tcaccattat caccaatgct ggtttgcgct gtgaccgggg tatcctctgg
    10501 cagttttttc atctctgcgc cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg
    10561 gcgaatcagg ttcgactgct tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc
    10621 gttatctggc gcatgcagtt tgtcttcatc acggctgaag gtgatggtgt atttaaagtt
    10681 catctcttta cccggctccg gcagctgatc cggcgtccag taagcgacga tgttatcgtt
    10741 ggtttcatcg ttggttggaa tttccaccag ctcaacgctg cctttacccc actccccttt
    10801 cggagtcacc catgcgcttg gacgaagatc gtaacgatca tcgagatctt caaagcggga
    10861 gaaatcacga ccgcgctgca acagaccgaa gccttgcggg ttctccatcg agaagctgct
    10921 gaccgctaaa tgtttcgggt tattcaacgg acgccagatc cattcgccat taccggcatg
    10981 gatagacaga ccgttagagt cgtgcaactc cggacgatag ttatttgcag gcgacggttg
    11041 gttcggccca aacaggaaca tactggttaa cggtgcaacc cccagtttgc cgactttatc
    11101 gcgcagatag attttcgact gcacatccac aaccgtgtca cgccctggca taaccacgaa
    11161 tttgtaagca cctgtcgcgc gcggcgagtc aagcaatgca taaatggtta aacgtttatc
    11221 agtcggtttt ggacgctcga tccagaactc tttgaagcgt ggaaattctt cacccgatgg
    11281 caaggcggta tcaattgcca ggccgcgggc agaaaggcca taaacctgac ctgcaccaat
    11341 cacgcggaaa tagctggccc cgagcatgct gacgatttca tcgtttttat ctttgctgtt
    11401 gatcgggtaa agcactttaa aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg
    11461 ctgaacatcg ccgaaagtga aataatccgg gctgtatttg attcgtttga ctgcggtggc
    11521 agtcacttca tttattttga ccggggtatc gaagtacata ccctgatggt agaactcgag
    11581 tttgaatggg gtcttcagat tgttccagta cgctttgtca tgattaaact ggatctgctg
    11641 atagtccgcg tatttcatat cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata
    11701 gcctttcccg gctaaggatt gagcttgctt tgcgacatca tcaatactga aagcccagct
    11761 tgaagatgta tacagggtta acattactgc agcactcaac caacgcattt tcatcatttg
    11821 tagtttatgt ttcataatta gtaagcactt ccccctttgt gtgcttatat cgatccgatc
    11881 tattttaatg gataaccagc cattccgaca acagaatccc ggtatcgttc agcacgctgg
    11941 ctcatggatt aagcacctga ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag
    12001 aagcatcgac ttcgtgtagg gtttgccagc aattttattt atcaaccgtc ttacagacgc
    12061 ttccattctg gttagtggta gcgatgatgc tatgcataaa aaccttgaag cctgcgcctg
    12121 gcgcggtcag gtcggtgtcg ctatggcaac aagcctaaag gcataacccg acagggataa
    12181 gacgaaaaat ccgagattag ttaaccatat atgaatccag tacccgcgca acgtgaatat
    12241 ttcctcgact ccatccgcgc ctggctgatg ttgttaggga tcccttttca tatttcttta
    12301 atctattcga gccatacatg gcatgtgaat agcgccgaac catcattgtg gctgaccctt
    12361 tttaatgact tcatccactc gttccgcatg caggtatttt tcgttatatc cggctacttt
    12421 tcctacatgc tttttttacg ctatcccttg aaaaaatggt ggaaagtacg tgtcgaacgt
    12481 gtaggtatcc cgatgttaac agccatcccc ctactgacat taccgcaatt tattatgctg
    12541 caatatgtca aaggaaaagc ggaaagttgg cctgggctgt cattgtatga caaatataat
    12601 acgttggcct gggaattaat atcacacctg tggtttttac tggtgttagt agtcatgacg
    12661 acgctgtgcg tatggatatt taagcgcatc agaaataatt tagaaaattc tgataaaacg
    12721 aataaaaaat tctcgatggt aaaactatcg gtgatttttt tatgcctcgg catcggttat
    12781 gcggtaataa gaagaacgat ttttattgtg tatccgccca ttctgagtaa tggcatgttc
    12841 aattttattg tcatgcaaac gctgttttat ttgccgttct ttatcctcgg cgcactggct
    12901 ttcattttcc ctcatcttaa agccttgttt accacgccgt ctcgtggctg tacccttgca
    12961 gcagcattgg cgtttgtcgc ttatttactc aaccagcgct atggcagtgg cgatgcctgg
    13021 atgtacgaaa ccgagtcggt gatcaccatg gtcctcggtc tgtggatggt gaatgtggtc
    13081 ttctcctttg gccaccgttt gcttaacttc cagtcagcgc gggtgactta ttttgttaac
    13141 gcatcgctgt ttatctatct ggttcaccac ccgttaacgc tgtttttcgg cgcatacatt
    13201 acaccgcaca tcacctccaa ctggcttggt tttctctgtg gcctgatatt tgtagtaggg
    13261 attgcgataa ttctgtatga aattcatttg cgcatcccgt tactgaagtt tttgttctct
    13321 ggtaaaccgg ttgttaagcg tgagaacgat aaagcaccag cccgttaagc cacatttaca
    13381 ataaccattc cacgggcaat atcgacgcca gtctgaccat aacccgcttc cagaaactgg
    13441 tggcgggttc ttttttgaga ataatctctt tctttgcatg acgatcaacc cagttgatcc
    13501 gtccccacct gtccagacgg agctgccagg ccgcatcata ctggctctga ataaagcgtt
    13561 tatcaattaa ctgtgccagc gtttcgctct ctatcacgaa gcccatttca gtattgagca
    13621 atgttgaacg cggatcgaaa ttgaaagagc cgataaacac cgttttacca tcgatgctaa
    13681 aggttttagc atgcaggctg gctccggaat taccggttat gccgcgatcg tgtaacgtac
    13741 tactttgttc acgcgtcggc ttgagttcat ataattccac gccatagcgg agcaattttt
    13801 tgcgccagcg cgcgtatccg gcatggacga cagcaacatc gttagcggca agagaattgg
    13861 ttaggatcgc aatctttacc ccttttctca ccatccgtaa gagttgcgcc acacctgcgc
    13921 gtgtcggtac aaaataggaa gagataatat cgatgcgttc actgggtgag cccatgatat
    13981 cgaacaggcg ctgcggtaac agtgaatgcc gttttgcctt gccctcccct ttcgccggat
    14041 catcacttaa taaacgtgtt ttcgcccaga taagcggcaa cgttccatca accagatgat
    14101 ttataaacgg actggattcc attttgcgta agtaacgatg cgtcatagca tcgttatgcc
    14161 aggaggcggg taactcgatg cgatccgcca tttcaccttc cgggacatcc agcacttgct
    14221 gtaagggtga aaccgatttg caataccagt agcgggcgaa atcatcggca acgtcctcta
    14281 ccacgggccc tattgccata acatctaaat ccgaaaaaag tggctcttcc cctgccccaa
    14341 aataggcatc accaatattt cgccccccta ccagcgtcac cacgccatcg acagtgaaac
    14401 ttttattgtg catacggcga ttgagacggg aaaagtcggt gatataacca agcggacgca
    14461 gcaagcgaaa cgagaaagga ttaaaaagcc ggacttcaat gcggggatgg ctgtcaagca
    14521 agcgtaaaat gtcgtcaagt ccgggcgtat tgttgtcgtc cagcaacaaa cggacgcgaa
    14581 cgccacgctt tgctgcggct aacagggcgg aaaacagtaa ccgacccgac atatcgtcct
    14641 gccagatgta atactgaaca tccagcgtat gctctgccat ttcggccaga cgataccggg
    14701 cggcaaacgc atccagactt ttctccagag ggaaaagacc acactgaccg ggatgttgcg
    14761 aacacagtgg cagcaccgcg ctcgccagcc ggggcaaatc attcatctcc ttgttgggta
    14821 aggagtcttt cgtagaggtg ggcgttttct tcatcataac agacaaagta tacctgttcg
    14881 ggtaaagcgt gacgggtaat aaattctgaa acggttttaa ctgcgatttc agccgctgcc
    14941 gcacgagggt aaccataaac cccagtactg attgcaggaa aagccactga cgtatagctg
    15001 tttgccgcca ccagtcgtag gctattgaga taggcatcct gcaaaagctg gtcttcgttt
    15061 tgttcaccac cacgccagac tggcccgacg gtgtgcacta cggctttagc gggaagagcg
    15121 cctgcaagcg taataacggc atgccccgta gggcaatcgc cctgctgttg cctgactttt
    15181 aaacaggcat ccagcagggc cggacccgct gcgcgatgaa tggccccatc gacgccgccg
    15241 cctcccatta atgacggatt agccgcattc acaatcacat caacggccag tttggtaata
    15301 tcaccctgca caacatgaat acgcgttttc ataacgcctc cttacacctt cttgtttctt
    15361 aagtgtacag caggcttttt ggttaaatcg catttttctg ctcagcaacg tgcgttgttc
    15421 agaaacttag ttatattcga tgttcattac agccagcttt ttctgtttat caagatactg
    15481 cacttttact gaggctattt gcgcgttttt aacattaccg ctcattaacg ctttgctgga
    15541 ataggttttg cccggaacag aaccattctg tgggcagttc atttctacgg gtgatgactg
    15601 ggtgctgacg tcacatgccg gttccacaat ttggccataa aaatggatca cccccccttg
    15661 cgcggtgctt cctgcgatgg caataaaagg aaaaagcaaa ctgccgagca taagagagtt
    15721 taaaaaaggt ctgaacattt attctatttc ctcaatgatt agtcatcctt gagggttgtg
    15781 ttatccatac tttcaggttg gctgattcgc agcagaccat tctctccaga ttcatcttat
    15841 gctcgatatt tcaacaaatt aagacttttc tgaagagggc ggccattgtt gtgataaatg
    15901 aagtgactgt ccatcagaaa cagtaacaac tattttcacc cgatcttccg gggaaatatt
    15961 taaactcaac ttcgtcaaag caataggttg attagcaggc aatgagaggg tcttttcttg
    16021 cttcgtctga ctttgccctg aactgccttc gcgcagggac aatatttgta ctctgcacag
    16081 acaagattga gtaagagtga cttcaggaat aatggtatac acatcccctt gctgggtcgt
    16141 attaaaggtt atctggctgg aaagtgccgc aaggagtaat aacgcattca tattcttctc
    16201 ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca aatgatgtat tagtactgat
    16261 gagcggtcgc gttgttacca aagccaacct gagtcacgtt gacggaggag ttagatgcag
    16321 tctggtcaac tgcagcaccg ttgccaccac cgaactgttt aacagtcatt tcagaatttt
    16381 tgccgttcca ctgatcaaga gtggcgctgt taccgaagcc acgttgggtc agatcgattg
    16441 agctgtcatc tgagccctga cctacatctg caccattacc accgccatgc tgggtaatag
    16501 tcaagtcaga gttacgggca tcagtttgca gagcaagtgc agagttaccg ccaccgtact
    16561 ggtaaatgtt cagctctgaa tttgggccgc tattattacc gccaccaccg tggttaccgc
    16621 cgccgccgta ctgaggaaca acacctgcca gagcgctacc ggagaatacg attgctgcaa
    16681 ttgctgctac ttttaaaagt ttcatgtaaa acccccatcg gattgattta aaagtcgaat
    16741 ggaaattaac gttgtgtcac gcgaatagcc atttgcgact gtctctgcac tacaattgcc
    16801 gttttttgag taccatactg tgtaatattt gctttattac cagaaccttt ctggataatc
    16861 atcgcagtat taccataagc accttgcgaa atactggcat cgttggcact gcccgcctga
    16921 tcaatatatg caaggttata atctcctgtc tggtcaatct ttgcccggtt gctactacct
    16981 tcttgcgcaa caaccgccaa aagttttgag cctccctgcc gtaactgagc actattattc
    17041 gtcccagctt gaccaattat ggctgcctga ttaaatgaag acttactcaa ttcatttacc
    17101 gcgaagttat attctgaatt agctaaatca taacctgctg cggctgcaat cccaggcgca
    17161 cccagtattg ttaacatcat aaataacaat ttgtttttca tgttgtcacc ctggacctgg
    17221 tcgtacattt aagaaattaa atcatttcaa cttggttgtt aacgcaacct gtattttgtt
    17281 aacgctgcgt tacgatggaa agtatgtctg cggaaatatt tttaataact cacccgcgcg
    17341 ttgtattttt ctttttttgt tcataccaaa gtattaaaac ccacgtaaat acgctgttat
    17401 ctacgcaaaa aatattttgt tttcttttaa atctccgttt tccgctatca aaaatcacca
    17461 gacagtcatt tttcttgccc gtcgctgatt gctgcgatat gtcttgcgca caagccgtga
    17521 caaacctcgt ctttttgcag taaaaaattg tccacggagg tgcggagaaa aaacaagaac
    17581 gttt
//
LOCUS       DAZFKM010000101        17563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFKM010000101 DAZFKM010000000
VERSION     DAZFKM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..1377)
                     /gene="gltA"
                     /locus_tag="ACHKAY_003535"
     CDS             complement(94..1377)
                     /gene="gltA"
                     /locus_tag="ACHKAY_003535"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO5702148.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1583..1690)
                     /locus_tag="ACHKAY_003536"
     CDS             complement(1583..1690)
                     /locus_tag="ACHKAY_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702149.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2071..2475
                     /gene="sdhC"
                     /locus_tag="ACHKAY_003537"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2071..2475
                     /gene="sdhC"
                     /locus_tag="ACHKAY_003537"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5702150.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2469..2816
                     /gene="sdhD"
                     /locus_tag="ACHKAY_003538"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2469..2816
                     /gene="sdhD"
                     /locus_tag="ACHKAY_003538"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO5702151.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2816..4582
                     /gene="sdhA"
                     /locus_tag="ACHKAY_003539"
     CDS             2816..4582
                     /gene="sdhA"
                     /locus_tag="ACHKAY_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO5702152.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4598..5314
                     /gene="sdhB"
                     /locus_tag="ACHKAY_003540"
     CDS             4598..5314
                     /gene="sdhB"
                     /locus_tag="ACHKAY_003540"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO5702153.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5615..8416
                     /gene="sucA"
                     /locus_tag="ACHKAY_003541"
     CDS             5615..8416
                     /gene="sucA"
                     /locus_tag="ACHKAY_003541"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO5702154.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8431..9648
                     /gene="odhB"
                     /locus_tag="ACHKAY_003542"
     CDS             8431..9648
                     /gene="odhB"
                     /locus_tag="ACHKAY_003542"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO5702155.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9742..10908
                     /gene="sucC"
                     /locus_tag="ACHKAY_003543"
     CDS             9742..10908
                     /gene="sucC"
                     /locus_tag="ACHKAY_003543"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO5702156.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10908..11777
                     /gene="sucD"
                     /locus_tag="ACHKAY_003544"
     CDS             10908..11777
                     /gene="sucD"
                     /locus_tag="ACHKAY_003544"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO5702157.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11881..12603)
                     /gene="mngR"
                     /locus_tag="ACHKAY_003545"
     CDS             complement(11881..12603)
                     /gene="mngR"
                     /locus_tag="ACHKAY_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO5702158.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12772..14688
                     /gene="mngA"
                     /locus_tag="ACHKAY_003546"
     CDS             12772..14688
                     /gene="mngA"
                     /locus_tag="ACHKAY_003546"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO5702159.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            14706..17339
                     /gene="mngB"
                     /locus_tag="ACHKAY_003547"
     CDS             14706..17339
                     /gene="mngB"
                     /locus_tag="ACHKAY_003547"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO5702160.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17364..>17563
                     /locus_tag="ACHKAY_003548"
     CDS             17364..>17563
                     /locus_tag="ACHKAY_003548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702161.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKI"
BASE COUNT         4250 a         4376 c         4772 g         4165 t
ORIGIN      
        1 ttttattttt acaatttttt gtgtttaggc atataaaaat taacccgcca tttgaacggc
       61 gggttaaaat atttacaact tagcaatcaa ccattaacgc ttgatatcgc ttttaaagtc
      121 gcgtttttca tatcctgtat acagctgacg cggacgggca atcttcatac cgtcactgtg
      181 catttcgctc cagtgggcga tccagccaac ggtacgtgcc attgcgaaaa tgacggtgaa
      241 catggaagac ggaataccca tcgctttcag gatgatacca gagtagaaat cgacgttcgg
      301 gtacagtttc ttctcgataa agtacgggtc gttcagcgcg atgttttcca gctccatagc
      361 cacttccagc aggtcatctt tggtgcccag ctctttcaga acttcatggc aggtttcacg
      421 cattacggtg gcgcgcgggt cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg
      481 gaaagaatca tttttatctt tcgcacgacg aacaaattcc ggaatgtgtt taacggagct
      541 aatttcttcc agcattttca gcgccgcttc gttagcacca ccgtgcgcag gtccccacag
      601 tgaagcaata cctgctgcga tacaggcaaa cgggttcgca cccgaagagc cagcggtacg
      661 cacggtggag gtagaggcgt tctgttcatg gtcagcgtgc aggatcagaa tacggtccat
      721 agcacgttcc agaatcggat taacttcata cggttcgcac ggcgtggaga acatcatatt
      781 caggaagtta ccggcatagg agagatcgtt gcgcggataa acaaatggct gaccaatgga
      841 atacttgtaa cacatcgcgg ccatggtcgg cattttcgac agcaggcgga acgcggcaat
      901 ttcacgatga cgaggattgt taacatccag cgagtcgtga tagaacgccg ccagcgcgcc
      961 ggtaatacca cacatgactg ccattggatg tgagtcgcga cggaaagcgt ggaacagacg
     1021 ggtaatctgc tcgtggatca tggtatgacg ggtcaccgta gttttaaatt cgtcatactg
     1081 ttcctgagtc ggtttttcac cattcagcag gatgtaacaa acttccaggt agttagaatc
     1141 ggtcgccagc tgatcgatcg ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat
     1201 aaaagtaatt ttagattcgc aggatgcggt tgaagtgaag cctgggtcaa aggtgaacac
     1261 accttttgaa ccgagagtac ggatatcaat aacatcttga cccagcgtgc ctttcagcac
     1321 atccagttca acagctgtgt ccccgttgag ggtgagtttt gcttttgtat cagccattta
     1381 aggtctcctt agcgccttat tacgtaagac tgccggaact taaatttgcc ttcgcacatc
     1441 aacctggctt tacccgtttg ttatttggct cgccgctctg tgaaagaggg gaaaacctgg
     1501 gtacagagct ctgggcgctt gcaggtaaag gatccattga tgacgaataa atggcgaatc
     1561 aagtacttag caatccgaat tattaaactt gtctaccact aataactgtc ccgaatgaat
     1621 tggtcaatac ttccacactg ttacataagt taatcttagg tgaaataccg acttcataac
     1681 ttttacgcat tatatgcttt tcctggtaat gtttgtaaca actttgttga atgattgtca
     1741 aattagatga ttaaaaatta aataaatgtt gttatcgtga cctggatcac tgttcaggat
     1801 aaaacccgac aaactatatg taggttaatt gtaatgattt tgtgaacagc ctatactgcc
     1861 gccaggtctc cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc
     1921 gcatctggaa gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc
     1981 aagcagaccg gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc
     2041 ccgtagtccc cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg
     2101 aaaaaacaaa gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata
     2161 gcgtccattc tccatcgcgt ttccggtgtg atcaccttta ttgcagtggg tatcctgctg
     2221 tggcttctgg gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg
     2281 ggcagcttct tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc
     2341 gtcgtaggta ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg
     2401 ggtaaacgct ccgccaaaat ctcctttgtt attactgtcg tgctttcact tctcgcagga
     2461 gtcctcgtat ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc
     2521 tcgttcgcgc taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg
     2581 ccaccagtgg cgaactgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca
     2641 aagtgttcac cctgctggcg ctgttttcta tcttgattca tgcctggatc ggcatgtggc
     2701 aggtgttgac cgactacgtt aaaccgctgg cattgcgcct gatgctgcaa ctggtgattg
     2761 tcgttgcact ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa
     2821 attgccagtc agagaatttg atgcagttgt gattggtgcc ggtggcgcag gtatgcgcgc
     2881 ggcgctgcaa atttcccaga gcggccagac ctgtgcgctg ctctctaaag tcttcccgac
     2941 ccgttcccat accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga
     3001 agataactgg gaatggcata tgtacgacac cgtaaaaggg tcggactata tcggtgacca
     3061 ggacgcgatt gaatatatgt gtaaaaccgg gccggaagcg attctggaac tcgaacacat
     3121 gggcctgccg ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt ttggcggtca
     3181 gtcgaaaaac ttcggcggcg agcaggcggc acgcactgcg gcggcggctg accgtaccgg
     3241 tcacgcactg ttgcacacgc tttatcagca gaacctgaaa aaccacacca ccattttctc
     3301 cgagtggtat gcgctggatc tggtgaaaaa ccaggatggc gcggtggtgg gttgtaccgc
     3361 actgtgcatc gaaaccggtg aagtggttta tttcaaagcc cgcgctaccg tgctggcgac
     3421 tggcggggca gggcgtattt atcagtccac cactaacgcc cacattaaca ctggcgacgg
     3481 tgtcggcatg gctatccgcg caggcgtacc ggtgcaggat atggaaatgt ggcaattcca
     3541 cccgaccggc attgccggtg cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg
     3601 ttatctgctg aacaaacatg gcgaacgctt tatggagcgt tatgcgccga acgccaaaga
     3661 cctggcgggc cgtgatgtgg tggcgcgttc catcatgatt gaaatccgtg aaggtcgcgg
     3721 ctgtgatggt ccgtgggggc cacacgcgaa actgaaactc gatcacctgg gtaaagaagt
     3781 tctcgaatcc cgtctgccgg gtatcctgga gctttcccgt accttcgctc acgtcgatcc
     3841 ggtgaaagag ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac
     3901 caaagttacc ggtcaggcgc tgactgtgaa tgagaaaggc gaagatgtgg ttgttccggg
     3961 actgtttgcc gttggtgaaa tcgcttgtgt atcggtacac ggcgctaacc gtctgggcgg
     4021 caactcgctg ctggacctgg tggtctttgg tcgcgcggca ggtctgcatc tgcaagagtc
     4081 tatcgccgag cagggcgcac tgcgcgatgc cagcgagtct gatgttgaag tgtctctgga
     4141 tcgcctgaac cgctggaaca ataatcgtaa cggtgaagat ccggtggcga ttcgtaaagc
     4201 gctgcaagaa tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa
     4261 agggcttgag cagttgaaag tgatccgcga gcgtctgaaa aatgcccgtc tggatgacac
     4321 ttccagcgag ttcaacaccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac
     4381 ggcgtatgca acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cacatagccg
     4441 cttcgacttc ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgccaga
     4501 gtcggaatcc atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc
     4561 gccgaagatt cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta
     4621 tcgctataac ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga
     4681 agaaggtcgc gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag
     4741 cctgtcgttc cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa
     4801 cggcaagaat ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa
     4861 gattgtgatt cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg
     4921 acaattctat gcgcaatatg acaaaattaa gccttacctg ttgaataatg gacaaaatcc
     4981 gccagctcgc gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggctgtatga
     5041 atgtattctc tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa
     5101 gtttatcggc ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac
     5161 cgagactgac agccgcctcg acggtttgag cgatgcattc agcgtattcc gctgtcacag
     5221 catcatgaac tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca
     5281 tattaagtcg atgttgttgc aacgtaatgc gtaaaccgta ggcctgataa gacgcgcaag
     5341 cgtcgcatca ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc ctacaagtca
     5401 ttacccgtag gcctgataag cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt
     5461 taaaaactgc ccctgacact aagacagttt ttaaaggttc cttcgcgagc cactacatag
     5521 acaagagctc gcaagtgaac cccggcacgc acatcactgt gcgtggtagt atccacggcg
     5581 aagtaagcat aaaaaagatg cttaagggat cacgatgcag aacagcgctt tgaaagcctg
     5641 gttggactct tcttacctct ctggcgcaaa ccagagctgg atagaacagc tctatgaaga
     5701 cttcttaacc gatcctgact cggttgacgc taactggcgt tcgacgttcc agcagttacc
     5761 tggtacggga gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt tccgccgcct
     5821 ggcgaaagac gcttcacgtt actcttcaac gatctccgac cctgacacca atgtgaagca
     5881 ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct
     5941 cgatccgctg ggactgtggc agcaagataa agtggccgat ctggatccgt ctttccacga
     6001 tctgaccgaa gcagacttcc aggagacctt caacgtcggt tcatttgcca gcggcaaaga
     6061 aaccatgaaa ctcggcgagc tgctggaagc cctcaagcaa acctactgtg gcccgattgg
     6121 tgccgagtat atgcacatta ccagcaccga agaaaaacgc tggatccaac agcgtatcga
     6181 gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc
     6241 cgctgaaggc cttgaacgtt acctcggcgc aaaattccct ggcgcaaaac gcttctcgct
     6301 ggaaggcggt gacgcgttaa tcccgatgct taaagagatg atccgccacg ctggcaacag
     6361 cggcacccgc gaagtggttc ttgggatggc gcaccgtggt cgtctgaacg tgctggtgaa
     6421 cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac ataaagaaca
     6481 cctcggcacg ggtgacgtga aataccacat gggcttctcg tctgacttcc agaccgatgg
     6541 cggcctggtg cacctggcgc tggcgtttaa cccgtctcac cttgagattg taagcccggt
     6601 agttatcggt tctgttcgtg cccgtctgga cagacttgat gagccgagca gcaacaaagt
     6661 gctgccaatc accatccacg gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac
     6721 tctgaacatg tcgaaagcgc gtggttatga agttggcggt acggtacgta tcgttatcaa
     6781 caaccaggtt ggtttcacca cctctaatcc gctggatgct cgttctacac cgtactgtac
     6841 tgatatcggt aagatggttc aggcaccgat tttccacgtt aacgcggatg atccggaagc
     6901 cgttgccttt gtgacccgtc tggcgctcga tttccgtaac acctttaaac gtgatgtctt
     6961 catcgacctg gtgtgctacc gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac
     7021 ccagccgctg atgtatcaga aaatcaaaaa acatccgaca ccgcgcaaaa tctacgctga
     7081 caagctggag caggaaaaag tggcgacgct ggaagatgcc accgagatgg ttaacctgta
     7141 ccgcgatgcg ctggatgctg gcgattgcgt agtggcagag tggcgtccga tgaacatgca
     7201 ctctttcacc tggtcgccgt acctcaacca cgaatgggac gaagagtacc cgaacaaagt
     7261 tgagatgaag cgcctgcagg agctggcgaa acgcatcagc acggtgccgg aagcagttga
     7321 aatgcagtct cgcgttgcca agatttatgg cgatcgccag gcgatggctg ccggtgagaa
     7381 actgttcgac tggggcggtg cggaaaacct cgcttacgcc acgctggttg acgaaggcat
     7441 tccggttcgc ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc gccacgcggt
     7501 gatccacaac cagtctaacg gttccactta cacgccgttg caacacatcc ataacgggca
     7561 gggcgcgttc cgcgtctggg actccgtact gtctgaagaa gcagtgctgg cgtttgaata
     7621 cggttatgcc accgcagaac cacgcactct gaccatctgg gaagcgcagt tcggtgactt
     7681 cgccaacggt gcgcaggtgg ttatcgacca gttcatctct tctggcgaac agaaatgggg
     7741 ccggatgtgt ggtctggtga tgttgctgcc gcacggttac gaagggcagg ggccggagca
     7801 ctcctccgcg cgtctggaac gttatctgca actttgtgct gagcaaaaca tgcaggtgtg
     7861 cgtaccgtct accccggcac aggtttacca catgctgcgt cgtcaggcgc tgcgcgggat
     7921 gcgtcgtccg ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc tggcggtttc
     7981 cagcctcgaa gaactggcga acggcacctt cctgccagcc atcggtgaaa tcgacgagct
     8041 tgatccgaag ggcgtgaagc gcgtagtgat gtgttctggt aaggtttatt acgacctgct
     8101 ggaacagcgt cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg agcaactcta
     8161 cccgttcccg cataaagcga tgcaggaagt gttgcagcag tttgctcacg tcaaggattt
     8221 tgtctggtgc caggaagagc cgctcaacca gggcgcatgg tactgcagcc agcatcattt
     8281 ccgtgaagtg attccgtttg gggcttctct gcgttatgca ggccgcccgg cctccgcctc
     8341 tccggcggta gggtatatgt ccgttcacca gaaacagcaa caagatctgg ttaatgacgc
     8401 gctgaacgtc gaataaataa aggatacaca atgagtagcg tagatattct ggtccctgac
     8461 ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca
     8521 gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca aagtggtact ggaagtaccg
     8581 gcatcagcag acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct
     8641 cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgccg gtaaagaaac cagcgccaaa
     8701 tctgaagaga aagcgtccac tccggcgcaa cgccagcagg catctctgga agagcaaaac
     8761 aacgatgcgt taagcccggc gatccgtcgc ctgctggctg aacacaatct cgacgccagc
     8821 gccattaaag gcaccggtgt gggtggtcgt ctgactcgtg aagatgtgga aaaacatctg
     8881 gcgaaagccc cggcgaaaga gtctgctccg gcagcggctg ctccggcggc gcaaccggct
     8941 ctggctgcac gtagtgaaaa acgtgtcccg atgactcgcc tgcgtaagcg tgtggcagag
     9001 cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg
     9061 aagccgatta tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc
     9121 cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg
     9181 gaagtgaacg cgtctatcga cggcgatgac gtggtttacc acaactattt cgacgtcagc
     9241 atggcggttt ctacgccgcg cggcctggtg acaccggtac tgcgtgatgt cgataccctc
     9301 ggcatggcag acatcgagaa gaaaatcaaa gagctggcag tcaaaggccg tgacggcaag
     9361 ctgaccgttg aagatctgac cggtggtaac ttcaccatca ccaacggtgg tgtgttcggt
     9421 tccctgatgt ctacgccgat catcaacccg ccgcagagcg caattctggg tatgcacgct
     9481 atcaaagatc gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg
     9541 gcgctgtcct acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg
     9601 atcaaagagt tgctggaaga tccgacgcgt ctgctgctgg acgtgtagta gtttaagctt
     9661 cacctgcact gtagaccgac atggataggg tgtcggtcta cggtttaaaa gataacgatt
     9721 actgaaggat ggacagaaca catgaactta catgaatatc aggcaaaaca actttttgcc
     9781 cgctatggct taccagcacc ggtgggttat gcctgtacta ctccgcgcga agcagaagaa
     9841 gccgcttcaa aaatcggtgc cggcccgtgg gtagtgaaat gtcaggttca cgctggtggc
     9901 cgcggtaaag cgggcggtgt gaaagttgta aacagcaaag aagacatccg tgcttttgca
     9961 gaaaactggc tcggcaagcg tctggtaacg tatcaaacag atgccaatgg ccaaccggtt
    10021 aaccagattc tggttgaagc agcgaccgat atcgctaaag agctgtatct cggtgccgtt
    10081 gtagaccgta gttcccgtcg tgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc
    10141 gaaaaagtgg cggaagaaac tccgcacctg atccacaaag ttgcgcttga tccgctgact
    10201 ggcccgatgc cgtatcaggg acgcgagctg gcgttcaaac tgggtctgga aggtaaactg
    10261 gttcagcagt tcaccaaaat cttcatgggc ctggcgacca ttttcctgga gcgcgacctg
    10321 gcgttgatcg aaatcaaccc gctggtcatc accaaacagg gcgatctgat ttgcctcgac
    10381 ggcaaactgg gcgctgacgg caacgcactg ttccgccagc ctgatctgcg cgaaatgcgt
    10441 gaccagtcgc aggaagatcc gcgtgaagca caggctgcac agtgggaact gaactacgtt
    10501 gcgctggacg gtaacatcgg ttgtatggtt aacggcgcag gtctggcgat gggtacgatg
    10561 gacatcgtta aactgcacgg cggcgaaccg gctaacttcc ttgacgttgg cggcggcgca
    10621 accaaagaac gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc
    10681 gttctggtta acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc
    10741 ggcgcggtag cagaagtggg tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac
    10801 gccgaactcg gcgcgaagaa actggctgac agcggcctga atattattgc agcaaaaggt
    10861 ctgacggatg cagctcagca ggttgttgcc gcagtggagg ggaaataatg tccattttaa
    10921 ttgataaaaa caccaaggtt atctgccagg gctttactgg tagccagggg actttccact
    10981 cagaacaggc cattgcatac ggcactaaaa tggttggcgg cgtaacccca ggtaaaggcg
    11041 gtactaccca cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt gctgctactg
    11101 gcgctaccgc ttctgttatc tacgtaccag caccgttctg caaagactcc attctggaag
    11161 ctattgatgc aggcatcaaa ctgattatca ccatcactga aggcatcccg acgctggata
    11221 tgctgactgt gaaagtgaaa ctggacgaag ccggcgttcg tatgatcggc ccgaactgcc
    11281 cgggcgttat cactccgggt gaatgcaaaa tcggtatcca gccgggtcac attcacaaac
    11341 cgggtaaagt gggtatcgtt tcccgttccg gtacactgac ctatgaagcg gttaaacaga
    11401 ccacggatta cggtttcggt cagtcgacct gcgttggtat cggcggtgac ccgatccctg
    11461 gctctaactt tatcgacatt ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg
    11521 taatgatcgg tgagatcggc ggtagcgctg aagaagaagc ggcagcgtac atcaaagagc
    11581 atgttaccaa gccagttgtg ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta
    11641 tgggccacgc gggtgccatc attgccggtg ggaaagggac tgcggatgag aaattcgctg
    11701 ctctggaagc cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt gaagcactga
    11761 aaactgttct gaaataaata tctgtaataa gaaatagccc tcgccgcttc cctctacagg
    11821 aatggcgaag ggctgtcggt ttcgacatgg ttggccatcg tatgatggcc ttttttgtgc
    11881 ttatcgcgat gattttcgtt gcgctatcag ggtaaattta tagtcatcgg tattaaaagc
    11941 gttgcggcta tattcaaaca cccgaccatc aactaaatat ccacgcgata ctttttcaag
    12001 aatcggcttt gtctggctga tattaagcag acggctcatc tcttcggttg gcatcagagg
    12061 aatgatttcc tgttcgctac gatcgataac cattttcttc acttcttcaa taaagtgata
    12121 ttttgaattt tccatgacct gccaggtgag atccgggaac aacgcaagcg gcatccaggt
    12181 ttcttccagc gccattggct tttgcttgcg atagcgcacg cgcttcacat gccacacacg
    12241 atcctgcgcg gtgatttgta gctgttgctg aagaaaatcg tcagccggaa tcacttcaaa
    12301 tatcaggact tcactgtgtg tatcgacgtg acggtccgac agtttttcat caaaactggt
    12361 taactgaaaa atatcgtaat tgacccgctc ttctttgacg taagtcccgc tgccctgaat
    12421 gctttcgagg atctgttgct cgactaactg gcgcaaagcc tgacgcaccg taacccggct
    12481 gacgccaaac tctgtttgta atgccgattc ggtaggtaac gcatcgccag gtttaagctc
    12541 gccacgcgca atttgttcac gaatgcgatc ggcaatctgc cggtataagg gcttgtgtcc
    12601 catttttagt atctcattaa tacgaattta gccattatgc ccgataaatt catcctgtaa
    12661 ataatacaaa tacaatacaa ataatttcaa tcaagtgaaa ttgatcacat aatggtattg
    12721 ttttatcggg cacactggcg cgactataaa aacgatcaag tgaggatcat gatgaatctg
    12781 acgactctga cccaccgcga tgcgttgtgt ctgaatgcgc gctttaccag ccgtgaagag
    12841 gccatccacg cgttgactca acgtcttgct gctctgggga aaatttccag tactgagcaa
    12901 tttctggaag aagtgtatcg ccgtgaaagc cttggcccga ccgccttagg tgaagggttg
    12961 gctgtgccgc atggcaaaac tgctgcggta aaagaagcgg cgtttgcggt cgccacactc
    13021 agcgagccgc ttcagtggga aggcgttgat ggcccggaag cggttgattt agtggtgctg
    13081 ctggcgattc cccccaatga agcgggtact acgcatatgc aactgctgac agcgctgacc
    13141 acgcgcctgg cggatgatga gattcgggcg cgtatacagt cggcgacgac gcctgatgag
    13201 ttgctctcgg cgctggatga caagggaggc acgcaacctt ctgcctcttt ttccaacgcg
    13261 ccaactatcg tctgcgtaac ggcctgtccg gcgggtattg ctcacaccta tatggctgcg
    13321 gaatatctgg aaaaagccgg acgcaaactc ggcgtaaatg tttacgttga aaaacaaggc
    13381 gctaacggca ttgaagggcg tttaacggcg gatcaactca atagtgcaac cgcctgtatt
    13441 tttgcggctg aagtcgccat caaggagagt gagcgtttta atggcattcc cgcgctttca
    13501 gtgcctgttg ccgagccgat tcgccatgca gaagcgttga tccaacaagc gcttaccctc
    13561 aagcgtagcg atgagacgcg taccgtacag caagatacgc aaccggtgaa aagtgtcaaa
    13621 acggagctga aacaggcact gttgagcgga atctcttttg ccgtaccgtt gattgtcgcg
    13681 gggggcacgg tgctggcggt cgcggtatta ctgtcgcaaa tcttcgggct acaagatctg
    13741 tttaatgaag aaaactcctg gctgtggatg taccgcaagc tgggcggcgg gctgctcgga
    13801 attttgatgg taccggtgct cgcggcctat accgcctatt ctctggcaga taaaccggcg
    13861 ttagcgccag gctttgcggc tggacttgcc gccaacatga tcggctccgg gtttctcggc
    13921 gcggtcgttg gcggattgat agccggttac ttgatgcgct gggtgaaaaa tcacttgcgt
    13981 cttggcagta aattcaatgg cttcctgact ttttatctct acccggtgct cggtacgttg
    14041 ggagcgggca gtctgatgct gtttgtggtg ggagaacctg tcgcatggat caataactcg
    14101 cttaccgcct ggctgaacgg tctgtcagga agtaacgcgc tgttgctggg tgccattctc
    14161 ggttttatgt gttcctttga ccttggaggg ccagtgaata aagccgctta tgcattctgc
    14221 ctgggcgcaa tggcgaacgg cgtttacggc ccgtatgcca ttttcgcctc cgtcaaaatg
    14281 gtttcggcat ttaccgtaac cgcttccacg atgctcgcac cgcgcctgtt taaagagttt
    14341 gaaattgaga ccgggaaatc cacctggctg ttagggctgg caggtattac cgaaggggcg
    14401 atcccgatgg cgattgaaga tccgctgcgg gttattggtt cgtttgtgct gggctctatg
    14461 gtaacgggcg ctattgtcgg tgcgatgaat atcggccttt cgacacccgg tgccggcatt
    14521 ttctcgctct ttttacttca tgataatggc gcgggcggtg ttatggcggc aattggctgg
    14581 tttggtgcgg cattggtggg ggctgcaatc tcgactgcaa ttctcctgat gtggcggcgt
    14641 cacgcggtta agcatggcaa ctatctgact gatggcgtaa tgccataaac aaaaaggaaa
    14701 cgacgatgaa agcggtatct cgcgttcaca tcaccccgca tatgcactgg gatcgggagt
    14761 ggtatttcac caccgaagag tcacgcattc tgctggtcaa taatatggaa gagatcctgt
    14821 gccgactgga acaggacaac gaatacaaat attacgtact cgacgggcaa acggcgatcc
    14881 tcgaagatta tttcgcggtg aaaccggaaa acaaagaccg tgtgaagaaa caggtagaag
    14941 ccggcaagtt gattatcggc ccctggtata cccagaccga taccacgatt gtttctgcgg
    15001 aatccatcgt ccgtaatctg atgtacggaa tgcgtgactg cctcgcgttt ggcgagccga
    15061 tgaaaatagg ttatttaccg gattcctttg gcatgtccgg gcagcttccg catatctaca
    15121 atggatttgg cattacccgc accatgttct ggcgtggatg ttctgagcgc cacggtactg
    15181 ataaaaccga gtttttgtgg caaagcagtg acggtagcga agtgacggcg caagtgttgc
    15241 cgctgggcta cgccatcggt aagtacttac ctgccgacga agacggatta cgcaagcgcc
    15301 tcgacagtta ttttgatgtg ctggaaaaag cgtcagtaac caaagaaatt ttattgccga
    15361 atggtcatga ccagatgcca ttgcagcaaa atatcttcga agtgatggat aagctacgtg
    15421 agatctaccc tcaacgtaag tttgtgatga gccgctttga agaggtattt gagaagatcg
    15481 aagcgcagcg agataatctg gcaaccctga aaggggaatt tattgatggc aaatatatgc
    15541 gcgtgcatcg caccatcggt tctacgcgta tggatatcaa aattgcccac gcgcgtattg
    15601 aaaataagat tgttaatctg ctggaaccgc tggcaacact ggcctggacg ttgggttttg
    15661 aataccacca cggcttgctg gagaaaatgt ggaaagagat cttaaaaaat catgcccacg
    15721 acagtatcgg ttgttgttgc agtgacaaag ttcatcgcga aatcgtcgcc cgcttcgaac
    15781 tggctgaaga catggcggat aatctgattc gtttctacat gcgcaaaatt gccgacaaca
    15841 tgccgcagag cgacgccgac aaactcgtcc tgtttaacct gatgccctgg ccgcgtgaag
    15901 aagtgatcaa caccactgtg cgcctgcgcg ccagccagtt taatttgcgg gacgatcgcg
    15961 gccagcctgt gccgtatttt attcgccatg cccgtgagat cgatccaggc ctaatcgatc
    16021 ggcaaattgt tcattatggc aattatgatc cctttatgga gttcgacatc cagatcaacc
    16081 agattgtccc ttctatgggc tatcgcacgc tttatattga agcgaatcag cctggcaacg
    16141 tgattgcggc aaaaagtgac gctgaaggga ttctggaaaa tgctttctgg cagattgcgc
    16201 tcaatgagga tggttctctg caactggtag ataaagacag cggtgtgcgc tatgaccggg
    16261 tattgcaaat tgaagaaagc tctgatgatg gtgatgaata tgactattca cccgcaaaag
    16321 aagagtgggt aattaccgcg gcgaacgcga aaccgcaatg cgatattatt catgaagcct
    16381 ggcagagcag ggctgttatc cgttatgaaa tggcggtgcc gcgtaatctg caagaacgca
    16441 gcgcaagaca atccactggc agagtagggg tggagatggt tgtcactctg agtcataaca
    16501 gcagacgtat tgatgtggat atcaaccttg ataaccaggc tgacgatcat cgccttcgcg
    16561 tcctgatccc tacacctttt aacaccgaca gtgttctggc agatacgcag tttggttcgc
    16621 taacgcgccc cgtgaacgac agtgcaatga acaactggca gcaagaaggc tggaaagaag
    16681 cgccggttcc ggtatggaat atgctcaact atgttgcctt acaggaaggg cgtaacggca
    16741 tggctgtctt tagcgaaggg ctacgtgaat ttgaagtcat cggtgaagag aagaaaacct
    16801 ttgccattac gttgctgcgt ggcgtgggct tactgggcaa agaagatctg cttttaaggc
    16861 ctgggcggcc ttcgggaatt aaaatgccag tcccggactc acaactacgt ggtctgcttt
    16921 cttgtcgcct aagtttattg agttataccg gtacgccaac cgccgctggt gtagctcagc
    16981 aggcgcgagc atggctgact ccagtacagt gttacaacaa aatcccatgg gatgcgatga
    17041 agctcaacaa agccggattc aacgtgccgg aaagttatag tttgttgaaa atgcccccag
    17101 tgggatgcct gataagcgca cttaagaaag ctgaagaccg acaagaagtg attttacggc
    17161 tgtttaatcc ggctgaatca gcaacctgtg atgcgactgt tgctttcagt cgcgaggtga
    17221 tttcttgctc agaaacgatg atggatgaac acattaccac cgaggaaaat caaggttcaa
    17281 atctatcggg gcctttttta cccggccagt cacggacgtt cagttaccgg cttgcctgaa
    17341 tagcaatcaa accgaagcca catatgcgcg gccagattgt tgacaaaggg cgctttgttc
    17401 atgccggata cggcatgaac gctttattcg gtctacaaaa gcaggcaaat tcaatatatt
    17461 gcagagatga tgtagacact ggcaagcgta gcgcatcagg taattttgcg tttatcttca
    17521 ctctcaagcc acgtatatgt ggctttattt ttaacaaaat aat
//
LOCUS       DAZFKM010000102        17324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFKM010000102 DAZFKM010000000
VERSION     DAZFKM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..306
                     /gene="cybC"
                     /locus_tag="ACHKAY_003549"
     CDS             <1..306
                     /gene="cybC"
                     /locus_tag="ACHKAY_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO5702162.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(351..815)
                     /gene="nrdG"
                     /locus_tag="ACHKAY_003550"
     CDS             complement(351..815)
                     /gene="nrdG"
                     /locus_tag="ACHKAY_003550"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO5702163.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(974..3112)
                     /gene="nrdD"
                     /locus_tag="ACHKAY_003551"
     CDS             complement(974..3112)
                     /gene="nrdD"
                     /locus_tag="ACHKAY_003551"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO5702164.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3506..5161)
                     /gene="treC"
                     /locus_tag="ACHKAY_003552"
     CDS             complement(3506..5161)
                     /gene="treC"
                     /locus_tag="ACHKAY_003552"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO5702165.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5211..6632)
                     /gene="treB"
                     /locus_tag="ACHKAY_003553"
     CDS             complement(5211..6632)
                     /gene="treB"
                     /locus_tag="ACHKAY_003553"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO5702166.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6751..7701)
                     /gene="treR"
                     /locus_tag="ACHKAY_003554"
                     /pseudo
     CDS             complement(6751..7701)
                     /gene="treR"
                     /locus_tag="ACHKAY_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7883..7936
                     /gene="mgtL"
                     /locus_tag="ACHKAY_003555"
     CDS             7883..7936
                     /gene="mgtL"
                     /locus_tag="ACHKAY_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO5702167.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8077..10773
                     /gene="mgtA"
                     /locus_tag="ACHKAY_003556"
     CDS             8077..10773
                     /gene="mgtA"
                     /locus_tag="ACHKAY_003556"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO5702168.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10980..11366)
                     /gene="ridA"
                     /locus_tag="ACHKAY_003557"
     CDS             complement(10980..11366)
                     /gene="ridA"
                     /locus_tag="ACHKAY_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO5702169.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(11439..11900)
                     /gene="pyrI"
                     /locus_tag="ACHKAY_003558"
     CDS             complement(11439..11900)
                     /gene="pyrI"
                     /locus_tag="ACHKAY_003558"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO5702170.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(11913..12848)
                     /gene="pyrB"
                     /locus_tag="ACHKAY_003559"
     CDS             complement(11913..12848)
                     /gene="pyrB"
                     /locus_tag="ACHKAY_003559"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO5702171.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(12852..12986)
                     /gene="pyrL"
                     /locus_tag="ACHKAY_003560"
     CDS             complement(12852..12986)
                     /gene="pyrL"
                     /locus_tag="ACHKAY_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO5702172.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(13267..13662)
                     /gene="yjgH"
                     /locus_tag="ACHKAY_003561"
     CDS             complement(13267..13662)
                     /gene="yjgH"
                     /locus_tag="ACHKAY_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5702173.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(13793..14506)
                     /gene="bdcA"
                     /locus_tag="ACHKAY_003562"
     CDS             complement(13793..14506)
                     /gene="bdcA"
                     /locus_tag="ACHKAY_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5702174.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            14577..15170
                     /gene="bdcR"
                     /locus_tag="ACHKAY_003563"
     CDS             14577..15170
                     /gene="bdcR"
                     /locus_tag="ACHKAY_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO5702175.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            15315..15767
                     /gene="tabA"
                     /locus_tag="ACHKAY_003564"
     CDS             15315..15767
                     /gene="tabA"
                     /locus_tag="ACHKAY_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO5702176.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            15890..>17324
                     /locus_tag="ACHKAY_003565"
     CDS             15890..>17324
                     /locus_tag="ACHKAY_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702177.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSNILI
                     DSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLIKKYEGEQLYLNELGYV
                     YGNEEKTNEIVKKLPGYVIKIFEEKMGN"
BASE COUNT         4278 a         4215 c         4436 g         4395 t
ORIGIN      
        1 aatatggaaa ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa
       61 gtcaaagacg cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg
      121 ccgaagctcg aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc
      181 gacattctgg tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaagtaaaa
      241 gaagcgcagg ctgctgcaga gcaactgaaa acgacccgca acgcctatca ccagaagtat
      301 cgttaattcc tcatttccct gttgcctgca ctcaggtaac agggaactta tcatcgcaaa
      361 tgatgcacca cctgattgct gctgccgcgc cagatcaggg aagggtcttt taaatcctgc
      421 acaaatttgc cgtcgaccag tacgttaatc aaatcaacaa cctgcatttg cgcagcgttg
      481 agttcgtcga gtttatagcc tgtccacacc cagatgtctt tacccggaca ctcggcgcgg
      541 atgcgtttta ccagtttcag aatatccggc acgttttgcg gatgcagcgg atcgccgccg
      601 gagagggaaa taccctggcg tttgatacga gtgtcattca gatcgttaat aatctggtct
      661 tccattgctt tagtaaatgt ctgaccggaa tttacccgcc atgtgctttt gttatagcaa
      721 ccggggcatt catgaacaca cccggagaca aacagggtgc agcgagtgcc ggggccgttg
      781 acgatgtcga caggatagta ctgatgataa ttcattgttt cgcctggctt atcagtccgc
      841 gtataggatg catccggtaa tcaacgcctg atgcgacgct gtcgcgtctt atcagaccta
      901 caatcgttgt tgccatgtag gccggataag gcgtttacgc cgcatccggc atttgcttaa
      961 cgtagagtga agattaacct atctgcccat tccccaaatg tttaacgcgg cgcttaactt
     1021 cttcctgctt accggcgtta aatggacgtg catccgggct acctaaatat ccgcacacgc
     1081 ggcgagtcac cgacacacgg gaggcgtcat ggttaccaca tttcgggcaa gtgaagcctt
     1141 tgctggtgca ctcgaactca ccggtaaagc cacactcgta gcactcatca atcggtgtat
     1201 tggtgccgta atacggtaca tgctgatagc tgtaatccca gacatcttcc agcgccttca
     1261 ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa accaccgttc gccagcggcg
     1321 ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac cttcttctcc acatcgaggt
     1381 ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg caccacgcca aactcagcag
     1441 tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg cgtgctgtag agactgaaac
     1501 cataacccgt ttcttctttc cactgatcca ctgcctggcg cagacgttca acaatggcga
     1561 tacctttcgc gcgaagctgc tcgttgtcgt aaacatgctc gccgccgaac agcgcgttaa
     1621 tggtttcgtg gatgccgatg taacccagcg aaatagacgc acgaccgttt ttgaagattt
     1681 cagaaacatc atcgtcagca ttcagacgta cgccacaagc accttccata tagaggatcg
     1741 gggccacgcg cgctttcacg ccttcgagac gagcgatacg ggtcatcagc gccttacgtg
     1801 ccagcaccag acgttcatcc agcagcttcc agaaggtggc ttcatcgcct tttgcttcca
     1861 gagcaatacg cggcaggttc aggctgatca cgccgaggtt gttacgacca tcgtggatct
     1921 gctcgccgtt ttcattttcc cacacgccga ggaagctgcg gcagcccatc ggggttttaa
     1981 acgaaccggt gactttcact acctgatcgt agttcaggat atccggatac atgcgcttgc
     2041 ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg atcgcctttt ttatggttca
     2101 gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc agttttacgg tttttaccga
     2161 ggcctgcgat acggttacgc aggatcgatt cctgaatcag gcgcgattcc cagctggtgc
     2221 ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt ggcggtgtgc agggtgttta
     2281 cttcgtactc cagcgactgg aaggcatcgt agcactcttt gatggtacga gagtttgcgt
     2341 agccttcggc gtccgggatg ttccactctt ctgcggtctt gcgatgtttg ttgtagctgg
     2401 cggtaacaaa cggcgccagc acttcatcga tacggttaat ggtggtgccg ccataaatat
     2461 ggctggcgac ctgagcaata atctgcgcag ttaccgcggt tgccgtagag atcgacttcg
     2521 gcggttcaat ctcggcgttc cccatcttaa agccctgggt cagcatgcct ttcaggtcga
     2581 tcaacatgca gttgaacatc gggaagaacg gcgagtaatc gagatcgtga tagtgaatat
     2641 cgccacgctc atgtgcctgc accacgtcac gcggcagcag gtgctgacgt gcatagtgtt
     2701 tagccacgat cccggccagc aggtcgcgct gggttggaat caccttgctg tctttgttgg
     2761 cgttttcgtt gagtaacgag gcgttggtct gctcgaccag accacggatc tcctggttca
     2821 ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg gtactcgatg taagcacgag
     2881 ccagttgttt gtatggaccc gacatcagct ggttttcaac tgcggtctgg atctcgttga
     2941 tatccacctg gttgcggccc tgcatctgct cgctgacaac cgcggcaaca gtggcgcaat
     3001 aatcggcatc atcgacttcc gctgctttag ctgcacgcag aatcgcttct ttgatgcgct
     3061 ctgatttaaa cggcactttg cagccgtctc gtttcatcac atgcggtgtc atgatcgctc
     3121 catattttta agaacaggtt atccacagaa attgggaaag gcgttttccg gttttttcgt
     3181 ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc cacaacgccg cacctttttc
     3241 gtggggagct gtagtagcaa ttatagtcga ttaatacaac atattgggtt gggacgcatt
     3301 ttaaagtcta tatatagtgc tttgcatcaa ggatgtttta actttttttg atgtagctca
     3361 aagtaaaaag cagagcgtac ggatgacggg cgctacagcg ttatgtaaat tttttaatga
     3421 atttgctggt tgaaaaatca acaaaaacaa catgctgaca gacaaaaccc cggaatgacc
     3481 ggcattaccg gggcttaggg aagatttact tctgtaacca ccagacagcc tcaaaaggcc
     3541 gtaaattcat ggcacagggt tgtggtgagg cttcttcgta gttatgcatc acaagctgcc
     3601 agttgccgcg catttgccct ggctgccagg gttggatctc acggctaagg ttggcaatga
     3661 ccagcaaggt ttgccccttc cattcacggc gatagcacca caatacaggg ctgtttggca
     3721 gcagatcctg gtaattgccc catgtcagga tggcttcttg cttacgcagt gcgattaact
     3781 tttggtaggt gtaaaacacc gaggagtcat cggccagcgc ggcttctacg ttgatttgtt
     3841 gatagttatc gcccaggcca atccacggtt cgccagccgt aaacccggca ttatcgccgt
     3901 tgctccattg catgggcgtg cggctgttgt cacgggattt actggcgagg attgccaata
     3961 actcgtcggc atcacgacca tcgttgcgca gctcggcaaa catattgagg ctctccacgt
     4021 cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat gccaatctct tcgccttggt
     4081 agatatacgg cgttccctgc atgccatgca gcaccatcgc cagcattttt gccgcaggca
     4141 cgcggtattc accttcatca ccaaagcgag aaacaatgcg cggctgatcg tggttacacc
     4201 agaacaaggc attccatgcc acgttgtgca ttccttgttg ccagtggcgg aacaatgttt
     4261 tcaacgccac aaagtcaggt ttagccagcg tccatttttc accaccggga taatcgacct
     4321 tcaggtgatg aaaattaaag gtcatcgaca attcactgcc tgtcagtgcc gcgtatcgct
     4381 ggcaatgctc aaggctggtg gaggacattt cacctacggt cattaacccg cgtggcgtaa
     4441 acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg tggcccgtcg gtgtagaagc
     4501 gacgcccgtc gccatccagg tcttcaggga aacgcgggtc tttggagatc agattcacca
     4561 catccaggcg caacccgtcg accccacgat cggcccagaa ttcacagact tttttcagct
     4621 ctgcgcgtac cgctggattc tcccagttga gatccgcctg ttctggtgca aagagatgca
     4681 aatagtactg ttcgctttcc gcatgccagc gccacgcact accgccaaat tttgaacgcc
     4741 agttgttcgg tggcgtttct ggttcgccat cgcgccagat ataaaactgg cggtaagggc
     4801 tttctttgtt cagcgcctcg cgaaaccagg catgttgggt agaggtatgg ttaaacacca
     4861 tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt caccagttcg tcaaaatcgt
     4921 ccagcgtgcc gtagatggga tcaatcgccg tatagttcgc tacgtcgtaa ccgttatcga
     4981 cctggggaga gatataaaag ggggttagcc agatggcatc aacgcccagt ttatgcagat
     5041 agtccaggcg ttggataacg ccacgtaaat cgccggtgcc gctacccgtg gtgtcctgaa
     5101 aactctttgg ataaatctgg tagataacgc cgttttgcca ccagtgggga agattagtca
     5161 ttacgttatt cctgcaaatg cgagggggcg caattgcgcc ccgaagaaaa ttaaacaata
     5221 tccagcgtgc ccaggcggta tttccgctga tagataaacg aggtgagtac aatcgggatg
     5281 atgatggcga taaccattgc cagcgcaaat acctgccagt agctcggttg aatcgagaga
     5341 attcccggca ggccgcctac gccgatacca ttcgccataa cgccgttcag gccgcatagt
     5401 aatcctgcca gaccagaacc aatcatcgcg cacagcatcg ggaagcgata tttcaggttg
     5461 atgccgtaca ttgcaggctc agtgacccca agccaggcgg agatagcggc aggcacggag
     5521 atctcgcgtt cattgtgctt gcggctggaa atgataatgc ctatcacggc ggagccctga
     5581 gcgatattcg acagcgcaat cagcggccac actggcgtgc cacccatgct ttgaatcatc
     5641 tgcaaatcaa tagcaagggt ggtctggtgt acaccggtga tcaccagcgg ggcgtacagg
     5701 aagccaaaca atgctgcgcc aatcggagca aagctgccgg tcatcaggtg acgtaccgca
     5761 aaggcaacgc catcgccaat catgcgacca aacggaccaa tcagcgcatg ggcgaggaac
     5821 accgcgagga tcagcgaaca gacgggcacc accaccagat agaggtaatc cggcacgatg
     5881 cgtttaaggc gagtttcaat aacgcccagc gccagcccgg ctaacagtgc cgggatcacc
     5941 tgcgcctgat agcctacttt ggcgatgctg aacatgccaa agtcccacac ttccggcagc
     6001 tgctgcccga gcagataagc gttcatcagc tggggagaaa ccagtgtcac gccaagcacg
     6061 ataccaagga tcggcgtgcc gcccattttt ttcaccgctg accagcaaat accgaccggc
     6121 aggtagaaga agatcgcttc accgatcaac cacagaaaat cgtagatcgt ttgcagggaa
     6181 gggtacattt gcgccagcgt ctgaccgttg ctcatgggca aatcgccgat cacattgcga
     6241 aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca gcgggaagaa gatctccgcg
     6301 aaatgagaga tcaactgctc atgccatttc atattctgcc gggcggcttt ttttacctgc
     6361 tctttatcaa cctgcgcctg tccggttgac gcaatcagtg cttgatagta atcacccaca
     6421 ttggtgccaa tcaccacctg aaattgcccg gcattggtga aacagccttt caccatgggg
     6481 agttgctcaa tttctttcgg tctggcattg gccggttggt tgaggacaaa gcgcaggcga
     6541 gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc cgaccagttc aatcaaccga
     6601 tcgatatccg tttggtttat tttgctcatc ataaagcccc atggcagatg acatttttgg
     6661 ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg gaacgttccc gaaacgcagc
     6721 gaagatcaca atttatcgtt caggaaacga tcaggacagg gtggcgggga tgatgatttg
     6781 ttgcggttcg ctgcgcccgg ttacctgtgc gatcaattgg caggccgcct ggcgtccagc
     6841 ttcggcgtaa ccaggatcta cggtgacgat ctccggatga aggaatttca ttaacggcgt
     6901 attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc tcttgcaggt atttacttgc
     6961 gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta gtttcaggcg taatcacttt
     7021 tgctacgttc tcatagcctt gcttcatagc aagccctggc agagcggcaa cgggatgcag
     7081 tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt cgcttaccgg tcgtcacgtc
     7141 actgtgcggc acgccgagat aactgatatt acgatgcccc tggtcataga gccgttgcat
     7201 caggatcttg attgcccctt cgtcgtcata acagaccgaa gcaaagcctt ttgcgtcacg
     7261 cgccagcaga accagcgatg actgccagtg ggctaacatt tcttctgtta tgccggtaaa
     7321 accgaacagc actacgccgt cgatattacg ccgttttagg atccccaaat gttcggcaac
     7381 taattgcggg gaaaactgac tttccatcat gattgggtcg taaccttgtt catagaacgc
     7441 tggcagcatg gtttgaacgg cgagattttc tgacaacgaa tccaggcggg taacaatgat
     7501 ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca gagcgggaag gggaaaatcc
     7561 atgctgattc atcactgctt ccacgcgctc gcgcgtgcgc tggctcacgc cgctttcgtt
     7621 attcagcacc cgggaaaccg tagatttccc cacgccgctt aagcgcgcga tgtctttgat
     7681 ggtcagccga ttttgcatcc tgttgtcccg taacgtgttg tttaattatt tgaggctaac
     7741 tttacccgtg cattcagcaa tgggtaaagt ctggtttatc gttggtttag ttgccagcag
     7801 gtattatatc gccataaatg ctacgaatat tattggattc tccttattat ttgcggcgct
     7861 tttttcactt accggaggtt atatggaacc tgatcccacg cctctccctc gacggagatt
     7921 aaaacttttt cggtaagccc gtcttttcac ggcgttaccg gatgcgtaag gccgtgacgt
     7981 tttaacgtcc ctgctcagct ttattacctt caggtaaggc ttcgccacgc ctgaagacat
     8041 ttctgtactg tttcagacag tgcggaggga ctccttatgt ttaaagaaat ttttacccgg
     8101 ctcattcgcc atttaccttc ccgtctggtt catcgtgatc cattgcctgg cgcgcagcag
     8161 acagtgaata cggtggtccc gccgtcctta agtgcgcatt gcctgaaaat ggcggtgatg
     8221 cccgaagaag aattgtggaa aacgttcgac acccatccgg aagggttaaa tcaggcggaa
     8281 gtggaatctg cccgcgaaca acatggtgaa aataaattac ccgcacaaca accgtcgccg
     8341 tggtgggtac atttatgggt ctgctatcgc aaccccttta atattttact caccattctc
     8401 ggcgctattt cttacgccac ggaagattta tttgccgcgg gcgttatcgc gctaatggtc
     8461 gccatttcta cgttgctgaa ctttattcag gaagcacgtt ccactaaagc ggcagatgcc
     8521 ctgaaagcga tggtcagcaa tactgcgacg gtgctgcgcg taattaacga caaaggcgaa
     8581 aatggctggc tggagatccc gatcgaccag ctggtgcccg gcgatattat aaaactggcg
     8641 gcgggggata tgatcccggc agatttacgt atcttgcagg cgcgggatct gttcgtcgct
     8701 caggcgtcgt taaccggtga gtctctgccc gtagaaaaag cagctaccac tcgccagccg
     8761 gagcacagca atccgctgga gtgcgacacc ctgtgtttta tgggcaccac cgtggtgagc
     8821 ggcacggcac aagcaatggt gattgctaca ggtgccaata cctggtttgg tcaactggcg
     8881 gggcgtgtta gtgagcagga aagcgagccg aatgcctttc agcaagggat cagccgcgtc
     8941 agtatgctgc tgattcgctt tatgctggtg atggctccgg tggtgctgtt aatcaatggt
     9001 tacaccaaag gcgactggtg ggaagcggca ctgtttgcgc tttcggtcgc ggtaggctta
     9061 acgccggaaa tgttgccgat gattgttacc tcgacgctgg cgcgcggcgc agtaaagctg
     9121 tcgaaacaga aagtgatcgt caaacatctg gatgctattc agaactttgg cgcaatggat
     9181 attctgtgca ctgataaaac tggcaccctg acacaggaca aaattgtgct ggagaatcat
     9241 accgatatct ccggtaaaac cagcgaacgc gtgctgcata gcgcgtggtt gaacagtcat
     9301 tatcagaccg gacttaaaaa cctgctcgat acagcggtgc tcgaaggtac ggatgaagag
     9361 tcagcgcgct cgctggccag tcgttggcaa aaaatcgatg agattccgtt tgatttcgag
     9421 cgtcgccgga tgtcggtggt agtggcagaa aataccgagc accatcagct ggtttgcaaa
     9481 ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc gtcacaatgg cgagattgtg
     9541 ccgctcgatg acatcatgct gcgtaagatt aagcgggtta ctgatacgct gaatcgtcag
     9601 gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag cgcgtgaagg agattaccag
     9661 cgggcggatg aatccgacct gatcctcgaa ggatatattg ctttccttga tccgccaaaa
     9721 gagacaactg ctccggcact gaaggcatta aaagcgagtg gaattaccgt aaaaatcctt
     9781 actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg aagtggggct ggacgcggga
     9841 gaggtggtca ttggtagtga tattgaaacg ctatctgacg acgaactggc aaatctcgca
     9901 cagcgcacca cgctgtttgc tcgtctgacg ccgatgcata aagaacgcat tgtgacctta
     9961 ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg gtattaatga tgcgcccgcc
    10021 ttacgcgctg cggatatcgg catttctgtg gacggcgcgg tagatattgc ccgtgaagcg
    10081 gctgatatca tcctgctgga aaaaagcctg atggtgctgg aagagggggt tattgaggga
    10141 cgtcgcactt tcgccaacat gctgaaatac atcaaaatga cggcgagctc taacttcggt
    10201 aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct tcctgccgat gttgccgtta
    10261 cacttgctta ttcagaacct gctgtacgat gtgtcacagg tggcgatccc gtttgataac
    10321 gtcgacgacg agcaaattca aaagccgcag cgttggaatc cggcggatct ggggcgcttt
    10381 atgatcttct tcggaccgat cagctcgatc ttcgatattc tgacgttttg cctgatgtgg
    10441 tgggtattcc atgccaacac gccggaaacg caaacgctgt tccagtcggg atggtttgtg
    10501 gtgggcttac tgtcgcaaac gctgattgtg catatgatcc gtacccgccg tgtgccgttt
    10561 attcagagct gtgcatcgtg gccgttaatg atcatgaccg tgatcgtgat gattgtcggg
    10621 atcgcattgc cgttttcacc gctggccagt tatctgcagc tgcaggcgct gccgttaagc
    10681 tatttcccgt ggctggttgc gattctggca gggtatatga cgttaaccca gttggtgaaa
    10741 gggttctata gccgtcgtta cggctggcag taaagaataa actgggcacg atagccccga
    10801 ttcagattgc agacaaagtg agcgttgttc atgccggatg cggcgtaaat gccttatccg
    10861 gcctacaaat tcaatcaatt gcaggaatca cgtagatcct gataagcgta gcgcatcagg
    10921 caattttgcg tttgtcatca gatttaatcc gggcatgata gcccggattt ccatcaagat
    10981 tagcgacgaa cagcgatcgc ttcgatctca atttttacgt ctttcggcag acgggcaact
    11041 tcaacgcaag aacgtgccgg gaaggtggca ttgtgttcgg tgaagaaggc ttcgtaggta
    11101 gcattaacgg ttgcgaagtc gttcagatct ttcacaaaca cggtggtttt aacgatgtcg
    11161 cccactttca ggcctgcggc ttcgacgatc gcttttacgt tatccagcga ctgacgtgcc
    11221 tgtgcagcga cgtctgccgg tacttcgccc gttttcggat ttaccgggat ctggccggag
    11281 gtgatgatca tattgcccag atcaacgccc tgtacgtaag gaccgatagc tgccggtgca
    11341 ttttccgtcg cgatagtttt gctcatgatt tctcctttat tacagcggta aaaagtctgg
    11401 ctcattatag ggagccagac ttttattacc aaccgcaatt aattggccag caccacatta
    11461 tgggaaaact ctttttcaca gtatttgcat ttgagcgcga tgtcattggc gcgttttcgc
    11521 acggcaaagc tggaagaaac cggttcggca tggctgatac agttgctgtt cgggcagacc
    11581 agcacattgt cgatgcgctc cggcagactg gggcgcgatt tacccaccac ttcatagttg
    11641 tcgatacggt taaccgtggc ttgcggcgca tacaatgcca gttgatctac ctgatcttca
    11701 ctcaaaaagg tattttcgat tttgatcaga tctttgcggc ccatctcgcc agaaggcagg
    11761 ttcagaccaa tggtgatgcg ctgatccgtt tcggtcagct tgaacagact caacagctta
    11821 aaaccgatct gggcggggat atggtcaatt accgtgccgc gtttaatagc ttcaacctgc
    11881 aatttattat cgtgtgtcat ctctatttcc ccttacagta ccagatcgcg attcagaacc
    11941 agtgccagta acgcctggcg agcgaaaatc ccgttgcctg cctgctggaa gtaccaggcg
    12001 tgtggcgttt tatcaacatc cgtcgcaatc tcatcaacac gcggcagcgg atgcagcact
    12061 ttcatattgg ctttggcgtt gtggagatcg ctggcgcgaa gaacaaactg cgctttcacg
    12121 ttggcgtact cggacgggtc cagacgctct ttttgcacgc gggtcatgta caggatgtct
    12181 acttccgcca tcacttcttc aatagagctg tgcagactcc atgcgatccc tttttcatcg
    12241 agcatatcca gaatgtattg cggcattgcc agcgcgtccg gcgcgatgaa gtaaaaacgg
    12301 ttgccatcga acttcgctaa cgcctgagtc agggagtgaa cggtgcggcc atatttcagg
    12361 tcaccaacca ttgcgacgtg gagattgtcc agacgcccct gggtttcctg aatagtgaat
    12421 aagtccagca aggtttgcgt cggatgttgg ttggagccat caccggcatt cagtaccggt
    12481 acattgccgg aaaactcggt ggccaggcgc gccgcacctt cctgcggatg acgcatcact
    12541 atcgcatcga cgtaagtgct gataaccgaa atggtatcgg ccagcgtttc gcccttttta
    12601 cccagtgatg tattggcgct gtcggagaag cccaccacgc tggcacccag gcggtgcata
    12661 gatgtttcga aagagaggcg ggtacgggta gaggcttcga agaaacagct ggcaatgact
    12721 ttgtgcttca acagctctgg ttgcgggttt gctttcagtt tcgccgctgt cgccagcacc
    12781 agattaaggt catcgcgact aaggtcgttt atggaaatga tatgtttctg atatagcgga
    12841 ttagccatct tttatctcct gacgcctggg caaaaaaaag cccctcgatt gaggggctgg
    12901 gaatgggtga tcaacgggaa gaaaaacggc aggccagcgt cttttttcag acgcggtaag
    12961 acaaaatgtc gaacacactg aaccatacat cctcccggca aattgtccgg cattatactc
    13021 atagccagaa gcggtgcaag catttgatgc aatattttgt cagcgcaaac ggtttatttg
    13081 aattaaaagt caaaatgtat gcatttttat ttgctttgat tctggaggag aactgtcgtt
    13141 cggtatcaat aattgcagac atttcctgct tttttctttt tcactttcac gcaatcagat
    13201 atgcatttta ttccattcat ccgacttata gggcggagtg tgaaaacgaa cggctaacac
    13261 tattgcttac agctcaggga tgcgcgctat cactttaatt tcaaaatcaa agcctgccag
    13321 ccaggtaacg cccaccgccg tccagtttgg ataaggaggg gcgctaaata tttcattttt
    13381 gaccgtcatg atgtcttcaa attgtttttc cggatcggta tgaaagctcg taacatcaat
    13441 gatatcgtca aaagtgcatc ccgcagcagc cagggtcgca tgcaaattat caaatgccag
    13501 tctgacttgt tgctgaaaat cgggttctgg tgttccgtcc tctcgacttc ctacttgccc
    13561 ggaaacaaac agcaaatcgc cggaacgaat agccgcagaa taacgatgct cagcatacag
    13621 tgaatgtcgg ccagcaggga aaacagcggt tctttctacc atttggttat cctcaagatt
    13681 tacgacatga acagaagatt tctctttacc gggagccgct tttagcggac gacgtgagta
    13741 aacaaaaccc agacatcatg gataatggct gggcttaatt gagcgtagtc ggttatgcgc
    13801 caaacgcgcc atcaatggta tgcatcgcgc cggtaacaaa actggcttct ggccctgcta
    13861 accatgcgac cataccagcg acctcttccg gttgcccatg tcttttgata gccatcaaac
    13921 tatgcaacat atcgcgcatt ggcccgttgg cgggattagc gtcggtatca attggccctg
    13981 gctggacgac gttaatggtg atcccacgcg gtccaaaatc acgggccagc ccgcgcgcca
    14041 tgccttgcag ggcagatttg ctggcggcat aagcagccat gcctgcaaca ggcatacgat
    14101 cgccattcac ggagccgatg attaagatgc gcccgccttc gggcatctgc cgggcggctt
    14161 caacagaggc atgataagga gcatgaatat tgattttgaa aaggcgatca atatcgtcgg
    14221 catttaattc cagggcatcg ccaaagacgc caatacctgc atttaccacc aggatatcca
    14281 atgcgccgct cttacgaacg acatcaatga cagcgtctct gtcagcacta tctgtaaata
    14341 ctgctgtcgc tccagtctct tgtgccagat gttcagcggc atctttcgac cccgcatagg
    14401 tgaatcgtac attggcccca tcagtgacga aacgacgtac gatagcggca ccgattccac
    14461 gactgccacc gaggatgaga actgtcttac ctgtaaaagc gcccataagg actccttgat
    14521 ttattatgta acatgcatta caaaactgtt ttaactttct gtcaacaagt tttgtaatgg
    14581 tcactaaaaa acaatctcgc gttccaggtc gtcccagacg tttcgctcct gagcaggcag
    14641 tctctgcggc aaaagtgctt tttcaccaaa aaggtttcga tgctgtcagt gttgctgaag
    14701 ttactgatta tcttggtatt aaccccccga gcctctacgc agcttttggc agtaaagttg
    14761 ggttatttag ccgtgtactc aatgaatatg tcggtacgga agctattccg cttgccgata
    14821 ttcttcgtga tgatcgtccg gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc
    14881 gcagatatag ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggt attcatagtc
    14941 atgatccaca agcgcgtgat attgccgttc aatattatca cgccgcagaa acgaccattt
    15001 atgactatat cgccaggcgg catccacaaa gcgcacaatg tgtgactgat tttatgagta
    15061 ccgtgatgtc agggctttct gcgaaggcac gggaggggca ctccatcgaa caactctgtg
    15121 caacagctgc actggcgggg gaagcgataa aaactattct caaggagtga tgctggcctt
    15181 gacccgaaag gcgggaacgc gcctgccgat aagttgtgat aagacaataa ttcacgcgtt
    15241 aaggctagcg gaattgatca tcttttcgta taacggtgga aatgaaacat tgttttaatt
    15301 aaggagtgga aaagatgatc atcggaaata ttcataatct tcagccgtgg ctaccccagg
    15361 agttacgcca ggcgattgag catatcaaag cgcacgttac ggcagaaacg ccaaagggta
    15421 agcacgatat cgaaggcaat cgtctgtttt atcttatctc ggaagatatg accgagccgt
    15481 acgaagctcg ccgtgcggag tatcatgccc gctatctcga cattcagatt gtgttaaaag
    15541 gtcaggaagg catgaccttc agcacgcaac ctgcaggcac gccggatacc gactggttag
    15601 ctgataaaga catcgcattt ttgccggaag gcgttgatga gaaaacagtt atccttaatg
    15661 aaggtgattt tgttgtgttt tatccggggg aagtgcataa accgctgtgc gcagtgggcg
    15721 cgccagccca ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga cttttcgccg
    15781 taaataactc caggtttacg gcgagtttgt gaaaagagcg ttttttgata tttttttgtg
    15841 agtagaattt gtaatgctta gacgttctta ttaactcaag gagtccgtca tggcaaaaat
    15901 atcagatttg aattgttatc aacacatggc atcagccgac aatgttaaac caaaaaatga
    15961 agtcttaaat acctggtgtg tgggaatgaa tgattttgct cgtaatgccg aggggcagga
    16021 tagcagaagg aatattctat ctcctggagc atttttggag tttttggcaa aaatatttac
    16081 cctgggttat gtggatttta gcaaacgctc caacgaagcg ggtagaaata tgatggctca
    16141 tattgagtcc tcatcttata tcaaaaataa tgatggcagt gaaataatga attttgttat
    16201 gaataatcct gaaggtgagc gtgcggattc atctaaggtg attatagaaa tttcactttc
    16261 tactaccact actatgggga ctcgtcaagg acatacagcc attatatttc cacaatctga
    16321 ttttttgagt tttcgttatg aaggtaagtc ttttgaaaga aaagatgata gttcattaca
    16381 tctgattact aacaaggttc tggcgtgtta ccaacgcgaa gctaacaaga atatggcgct
    16441 tctattaaat aatcatcagt tgaactggaa ggacgcaatt gattataacg agttattaga
    16501 aaagccatta gccagtacat tagaaaaaat aaagaaagaa catctgctat taatgcctca
    16561 tgtatgtgat gacacgattc catacttact aggcgaaggt ggtatacttg aagaaataaa
    16621 tgatctgaaa gcgttacatg ataccagaat tgctagtggt acagaaggta atgatgaaat
    16681 taataacatc aaaattaacc tgtctaatat tcttattgat tccttagatg atgcaaaagt
    16741 taatctcgaa aaggtcatcg attcgatgtt gaagactttt ttaaaattgc catatattaa
    16801 tgatgtaaaa attctggagt ggtgttttaa tcaaagtatg caatattctg atgatactac
    16861 aaagataaag catgcccgct ccgtgatcga tcatattgat tttaactgcg atcagtctaa
    16921 aatagctgag acattatttt tcaacctcaa taaagaaccc tataaaaaca gccctgagtt
    16981 acaggaattg atttgggaga agttggttgt atatgtcaat gattttaact taagcaatca
    17041 agaaaaatca cgcttaatac taaggttatt tgatgatgtt aagttgctat ttaatgaagt
    17101 gcctgtcagc attttagtaa atgatatttt tttgaaggat tttttcatga agcaacctga
    17161 ttttgcgaag tggtacttct atcaattaat taagaagtat gagggcgaac agctgtatct
    17221 taatgagtta ggatatgttt atggtaatga agaaaaaaca aatgaaattg taaaaaaact
    17281 tccgggttat gtcattaaga tttttgaaga aaagatgggt aatg
//
LOCUS       DAZFKM010000103        17214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFKM010000103 DAZFKM010000000
VERSION     DAZFKM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..599
                     /locus_tag="ACHKAY_003566"
     CDS             15..599
                     /locus_tag="ACHKAY_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702178.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            679..1374
                     /locus_tag="ACHKAY_003567"
     CDS             679..1374
                     /locus_tag="ACHKAY_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5702179.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1404..3920
                     /locus_tag="ACHKAY_003568"
     CDS             1404..3920
                     /locus_tag="ACHKAY_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5702180.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3931..5022
                     /locus_tag="ACHKAY_003569"
     CDS             3931..5022
                     /locus_tag="ACHKAY_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702181.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5065..5925)
                     /gene="rsmI"
                     /locus_tag="ACHKAY_003570"
     CDS             complement(5065..5925)
                     /gene="rsmI"
                     /locus_tag="ACHKAY_003570"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO5702182.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5989..8025
                     /gene="lpoA"
                     /locus_tag="ACHKAY_003571"
     CDS             5989..8025
                     /gene="lpoA"
                     /locus_tag="ACHKAY_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO5702183.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7983..8378
                     /locus_tag="ACHKAY_003572"
     CDS             7983..8378
                     /locus_tag="ACHKAY_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO5702184.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8398..8988
                     /gene="diaA"
                     /locus_tag="ACHKAY_003573"
     CDS             8398..8988
                     /gene="diaA"
                     /locus_tag="ACHKAY_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO5702185.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            8998..9573
                     /gene="dolP"
                     /locus_tag="ACHKAY_003574"
     CDS             8998..9573
                     /gene="dolP"
                     /locus_tag="ACHKAY_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO5702186.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9687..10727)
                     /locus_tag="ACHKAY_003575"
     CDS             complement(9687..10727)
                     /locus_tag="ACHKAY_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO5702187.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10800..11435)
                     /locus_tag="ACHKAY_003576"
     CDS             complement(10800..11435)
                     /locus_tag="ACHKAY_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO5702188.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11563..12081
                     /gene="yhbO"
                     /locus_tag="ACHKAY_003577"
     CDS             11563..12081
                     /gene="yhbO"
                     /locus_tag="ACHKAY_003577"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO5702189.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12061..12504)
                     /locus_tag="ACHKAY_003578"
     CDS             complement(12061..12504)
                     /locus_tag="ACHKAY_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO5702190.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12555..12857
                     /gene="yhbQ"
                     /locus_tag="ACHKAY_003579"
     CDS             12555..12857
                     /gene="yhbQ"
                     /locus_tag="ACHKAY_003579"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO5702191.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12844..13347)
                     /locus_tag="ACHKAY_003580"
     CDS             complement(12844..13347)
                     /locus_tag="ACHKAY_003580"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5702192.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13341..13865)
                     /locus_tag="ACHKAY_003581"
     CDS             complement(13341..13865)
                     /locus_tag="ACHKAY_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO5702193.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14074..15069
                     /locus_tag="ACHKAY_003582"
     CDS             14074..15069
                     /locus_tag="ACHKAY_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO5702194.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15078..15956
                     /locus_tag="ACHKAY_003583"
     CDS             15078..15956
                     /locus_tag="ACHKAY_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO5702195.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16162..17169
                     /locus_tag="ACHKAY_003584"
     CDS             16162..17169
                     /locus_tag="ACHKAY_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO5702196.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4339 a         4561 c         4356 g         3958 t
ORIGIN      
        1 ggataaggtt ataaatgaat aaagttacaa aaacagctat tgtgggctta cttgcgcttt
       61 tcgcaggtaa tgccgctgca accgatggtg aaatagtttt tgatggcgag attttaaaat
      121 ccgcttgtga aatcaatgac tctgataaga aaattgaagt tgctcttggt cactataatg
      181 ccgaacagtt tcgttctgtt ggcgatcgta gcccaaaaat tccattcact attcctttag
      241 ttaactgccc agtgactggg tgggagcacg ataacggcaa cgttgaagcg tctttccgtt
      301 tatggctgga aacgcgggat aatggcaccg tccctaactt ccctaatctg gcaaaagtgg
      361 gctcttttgc tggcacagcg gcaacaggtg tgggtatccg tattgacgat gcggaaagcg
      421 gaaaccttat gccactgaat gctatgggga atgataacac ggtttatcag atcccagcgg
      481 actccaatgg tattgtcaat gttgacctca tcgcttacta cgtatcaacc gttgaagcat
      541 cagaaatcac cccaggggaa gcagacgctg tcgttaacgt aacgctggat taccgttaat
      601 acgttacggc gttatctgac ctttcagata acgccctttt ccttcctctt tctcgttgta
      661 tcaggttgaa aaataactat gtcaaaacga acattcgcgg tgataataac cttgttgtgt
      721 agcttctgta tcggccaggc gcttgcaggg ggtatcgttt tacagcgaac gcgagtgatc
      781 tatgatgcca gccgcaaaga ggctgcgtta cctgtcgcaa acaaaggcgc agaaacgcct
      841 tatttactgc aatcatgggt agataatata gatggtacaa gccgtgcccc atttattata
      901 accccaccgc tatttcgtct tgaagccggc gatgactcat cactgcgaat tattaaaaca
      961 gctgataacc tgcctgaaaa taaagagtcg ctgttctaca ttaatgttcg tgccattcca
     1021 gcaaagaaaa aatcagatga tgttaatgct aacgagttga cgctggtatt taaaacacgg
     1081 atcaaaatgt tttatcgccc cgcacacctg aagggacggg taaacgatgc gtggacatca
     1141 ctggaattta aacgtagtga ccattcactc aatatatata acccaactga atattacgtc
     1201 gtatttgccg gactggcagt cgataaaacc gatctcacaa gcaaaattga atatatcgcg
     1261 cccggagaac ataaacagtt accacttcct gcatctggcg gaaagaacgt gaaatgggct
     1321 gcaatcaatg attatggcgg cagttccggg acagaaactc gtccactgca ataacaaata
     1381 taaaaaacac aggtcatcag ggaatgccac aacgacacca ccagggacat aaacgcacac
     1441 cgaaacagtt ggcgctcatc atcaaacgct gtttgccgat ggtgctcact ggcagcggca
     1501 tgctttgcac taccgctaac gccgaagagt attatttcga ccccattatg ctggaaacta
     1561 caaaaagtgg tatgcaaaca actgatctgt cacgtttttc aaaaaaatac gcacaactac
     1621 caggaactta tcaggttgat atctggctga ataaaaagaa ggtttcacag aaaaaaatta
     1681 catttaccgc caatgcagag caacttctgc agccacagtt tacggtagaa caactacgtg
     1741 agctgggtat taaggtggat gaaatcccgg cgctggctga aaaagatgac gatagcgtga
     1801 tcaactcgct tgaacaaatc attcccggta cagctgctga atttgatttc aatcatcagc
     1861 gacttaattt gagcattccc caaattgcac tgtaccgtga tgcaagaggt tacgtctccc
     1921 cttctcgttg ggacgatggt ataccaacgc tgtttaccaa ctactcgttt acaggttctg
     1981 ataaccgtta ccgccagggc aatcgtagcc aacgacagta cctgaatatg caaaatggtg
     2041 ctaattttgg cccctggcga ttacgtaact attctacgtg gacacgcaac gatcaggcgt
     2101 caagctggaa cactatcagt agttatttac aacgtgatat caaggcgttg aagtctcagt
     2161 tgcttctggg agaaagcgcc accagcggca gtattttttc cagctacacc tttactggcg
     2221 tgcaactcgc ttccgacgat aatatgttgc caaacagcca gcgcggattt gccccaacgg
     2281 tacgcggtat cgcaaacagt agtgcaatcg tgactatcag gcaaaatggt tatgtgatct
     2341 atcaaagcaa cgtgccagcg ggtgcctttg aaattaacga tctctacccc tcttccaaca
     2401 gcggcgattt agaagtcacg attgaagaaa gtgacggtac gcaacgtcgc tttatccagc
     2461 cttattcttc attacccatg atgcagcgac ctgggcatct aaaatatagc gcgaccgctg
     2521 gacgctatcg cgctgatgca aacagtgata gcaaggaacc cgaatttgct gaagccacgg
     2581 caatatatgg tttgaataat acttttacgc tgtatggcgg cctgctcggt tctgaagatt
     2641 attatgcgct ggggatcggt atcggcggca cacttggcgc actgggcgcg ttgtcgatgg
     2701 atatcaacag agctgacacc caattcgata accggcactc ttttcatggc tatcaatggc
     2761 gtacgcagta catcaaagat atcccggaaa ccaacaccaa tatcgctgtt agctactatc
     2821 gctataccaa cgatggctat tttagttttg atgaagccaa tacccgtaat tgggactata
     2881 acagtcgcca aaaaagtgaa attcaattca acatcagcca gacaatattt gatggggtaa
     2941 gtctgtatgc ctccggttcg cagcaagact attggggcaa taacgataaa aacaggaata
     3001 tctcagttgg ggtttccggt cagcaatggg gaattggtta cagcctgaat tatcaataca
     3061 gtcgctacac ggatcaaaat aatgaccgcg cactctcttt gaatctcagt attccgttag
     3121 aacgctggtt accgcgtagc cgggtttcct atcagatgac cagccagaaa gatcgcccaa
     3181 cccaacatga aatgcgtctt gatggctcac tgctggatga tggtcgcctg agctatagcc
     3241 tggaacaaaa tctggatgac gataacaacc ataacagtag cctgaacgcc agttaccgtt
     3301 caccttatgg caccttcagt gccggataca gctacggtaa tgacagcagc caatacaatt
     3361 acggcgttac cggcggcgtg gttatccatc ctcatggcgt gacgctctcg caatatctgg
     3421 gcaacgcttt tgcgcttatc gatgctaatg gggcttctgg cgtgaggata caaaactatc
     3481 cggggattgc tactgatccc tttggctatg cagtggttcc ttatctcacg acttaccagg
     3541 aaaaccgtct ctcggtagat actacgcagc tgcccgataa cgtcgatctt gagcaaacaa
     3601 cacagtttgt ggtgcccaac agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg
     3661 gttatcgcgt acttgttaca gtcagcgatc gcaacggtaa accgttgccc tttggcgctc
     3721 ttgccagcaa cgatgagacg gggcaacaaa gtatcgtcga tgagggcggc atactatatc
     3781 tctctgggat atcgagtaaa tcacaaagct ggactgtacg ctggggaaat caggcagatc
     3841 aacaatgtca gtttgctttt agtacaccgg attcagaacc aacaacctct gtattacaag
     3901 gcacggcgca gtgccattaa ggataaaaaa atgaaaagag cgcctcttat aacaggactt
     3961 ttgttgatat ccacatcctg cgcttatgcc tcctcaggag ggtgtggagc tgacagcact
     4021 agcggtgcga caaattacag cagtgtggtt gatgatgtta cggtgaacca gacagataac
     4081 gtgacaggac gggagtttac ctctgccacg ctaagtagca ctaactggca atacgcctgt
     4141 acctgctctg cgggtaaggc agttaaactt gtctatatgg tcagccctgt acttaccacc
     4201 actggacatc aagcaggata ttacaaactc aatgatagtc tggatattaa aaccacatta
     4261 caggcgaatg acattccagg actcgtgact gaccagaccg tttctgttaa cacccgattc
     4321 acacagataa aaagcaacac cgtatattct gctgcaaccc aaacgggtgt ttgccagggt
     4381 gacacgtctc gttatggacc cgttaatatt ggtgcaaata ccacctttac cctgtatgtc
     4441 accaagccat ttctcggctc gatgaccatt ccgaaaacgg atattgccgt cattaaaggc
     4501 gcatgggtcg atggaatggg aagcccgtct acaggtgact tccatgattt agtcaagtta
     4561 tcgattcagg gaaatctcac cgccccacag tcgtgcaaaa ttaatcaggg cgatgttatt
     4621 aaggttaatt ttggattcat caatggtcag aagtttacca cccgcaatgc catgccagac
     4681 ggttttactc cagtagactt tgatatcact tatgactgtg gtgatacttc aaagattaaa
     4741 aactcgttgc aaatgcgcat cgacggtaca actggggtag tagaccagta caacctggtc
     4801 gccagacgaa gaagttcaga caatgcgccc gatgtcggta ttcgtattga aaatctcggc
     4861 ggcggagttg caaatattcc ttttcagaac ggtatccttc ccgttgatcc ttccgggcat
     4921 ggcaccatca acatgcgcgc ctggccagtt aatctggtcg gtggtgagct ggaaacagga
     4981 aaatttcagg gcaccgccac cattaccgtc atcgtgcggt aaaagaagac gttgcgtttt
     5041 gattaacgca acgcttcggc actcttaccc ctgctgctcc agcgcatact tatacagcgc
     5101 atttttcttc acgccgtgaa tttctgccgc cagcgccgcc gcttttttca acggcagttc
     5161 tgcctgtagc agcgccagcg tgcgcagggc atcggcgggt aagtcttctt cctgtgcttt
     5221 atgaccttcg ataatcagca ccatttcgcc tttgcgacgg ttttcatctt cttttaccca
     5281 cgccagcagc tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg tcagctcacg
     5341 cgccagaacc acgtagcggg attcgcctaa taccgcaacg atatcttcca ggctatctaa
     5401 cagacggtgg gtggattcat aaaaaatcag cgtgcgcggc tccgcttcaa tggcttttag
     5461 cgcatcacgg cggccttttg atttggcagg taaaaagcct tcgtaacaga aacggtcaga
     5521 gggtaaaccc gcggcgctta acgcagtgat agcagcacac ggcccgggta gcggcaccac
     5581 gcaaatcccc gcttcacggc aggtacgcac cagatggtag ccaggatcgt taattagcgg
     5641 cgttccggca tcggaaacca gcgcaatgtt ctgcccttct tgcagcttcg ccagcagcgt
     5701 ttcggctttt tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat taatcccaaa
     5761 atgttgcagc aataaaccgg tgtgacgagt atcctcggcg gcaatcagat caacggcctg
     5821 taatacctct aacgcacgct gggtgatatc cgccagattg ccgattggcg tcggtacaat
     5881 gtaaagctgg ccctgagaat tatccgccga ttggtgttgt ttcattgtgt cgtccgtatt
     5941 gccgatttaa tattgagcat tgcgtaaaaa aatatcactg gatacattat ggtaccctca
     6001 acattttctc gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc cctgattttc
     6061 gccggttgtg gcacccatac tcccgatcag tccactgctt atatgcaggg cacggcgcag
     6121 gctgattctg ccttttatct tcagcagatg cagcaaagct ctgatgatac caggatcaac
     6181 tggcaattac tcgccattcg tgcactggtg aaagaaggta aaaccgggca ggcggttgag
     6241 ttgtttaacc aactaccgca agaactgaac gattctcagc gtcgcgagaa aacactgctg
     6301 gcggtagaga ttaaactggc gcagaaagat tttgctggcg cgcaaaactt gctggcgaaa
     6361 atcacacctg ccgatttaga acaaaaccag caagcgcgtt actggcaggc aaaaatcgat
     6421 gccagccagg ggcgtccttc cattgattta ctgcgcgcgt taattgctca ggaaccgctg
     6481 ctcggcgcga aagaaaaaca gcagaatatt gatgccacct ggcaggcgct ctcctccatg
     6541 actcaggaac aggcgaatac gctggtgatc aacgccgacg aaaatattct gcaaggctgg
     6601 ctggatctgc agcgcgtctg gtttgataac cgtaacgatc ccgacatgat gaaagccggg
     6661 atcgccgact ggcaaaaacg ttatccgaac aatccgggcg cgaaaatgct gccaacgcag
     6721 ttggttaacg taaaagcgtt taaaccagcc tcgaccaaca aaatcgccct gctgttgccg
     6781 ctgaatggcc aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa
     6841 aatatcggca ctcagccagt ggcggctcag gtagctgccg cacctgccgc agacgtagct
     6901 gaacaacctc agccgcaaac cgtggatggc gttgccagcc cggcacaagc ctcggttagc
     6961 gatctgaccg gtgaacagcc tgcggcacag ccagcgcctg taagcacccc ggcagcaacc
     7021 accacagcgg taagcgcgcc agcgaatcca tccgcagaac tgaaaatcta cgatacctca
     7081 tcacaaccac ttagccagat cttaagccag gttcagcagg atggcgcgag tattgtggtt
     7141 ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga gcaacacccc gctgaacgtg
     7201 ctggcactga accagccgga gaatatcgaa aaccgcgtca atatttgtta cttcgcgctt
     7261 tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg
     7321 ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg tagccaatgc gtttgcgcag
     7381 gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa aatttggttc caccagcgaa
     7441 ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa cgggtacccc gattactccc
     7501 agagcgacaa ccgactccgg catgacgacc aacaatccaa cgctgcaaac cacgccaacc
     7561 gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt ggcaacgccg
     7621 ggtgaaatcg cttttatcaa accgatgatc gccatgcgta acggtagcca gagcggtgca
     7681 acgctgtacg ccagctcccg cagtgcgcaa gggaccgctg gcccggattt ccgactggag
     7741 atggaaggct tgcagtacag cgaaatcccg atgctggcag gcggtaatct accgttaatg
     7801 cagcaggcac tcagcgcggt gaataacgat tattcactgg ctcgcatgta tgcgatgggc
     7861 gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc gccaggttca gggttttgaa
     7921 atcaacggta ataccggaag cctgacggca aacccggatt gcgtgattaa caggaagtta
     7981 tcatggctac agtaccaaca aggtcaggta gtccccgcca gttaaccacc aaacagaccg
     8041 gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg caaaggactg cgatttgtcg
     8101 ccgctaacgt gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa ggccggacca
     8161 ccgtttttgt cgaggtacgc tatcgccgct ctgcgcttta tggcggcgcg gcagccagtg
     8221 tgacccgcag caaacaacac aaattattac agactgcccg cttgtggctc gcgcgtcata
     8281 atgggagttt tgatactgtg gattgccggt tcgatgtggt agccttcacc gggaatgagg
     8341 ttgagtggat taaggatgcc tttaatgacc actcataatt aaggtttaag gattagcgtg
     8401 caagaaagaa ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc ggcggcagag
     8461 gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg ttcagtctct gctcaatggc
     8521 aacaaaatcc tctgttgtgg taatggaact tccgctgcca atgcacagca ttttgctgcc
     8581 agcatgatca accgtttcga aacggagcgg cccagcttac ctgccattgc actaaatact
     8641 gataatgttg tcttaacggc gattgccaac gatcgcttac atgatgaagt gtatgcaaaa
     8701 caggtgcggg cgctgggtca tgcgggagat gtattgttag ccatttccac ccgtggcaac
     8761 agccgcgata ttgtaaaagc ggtagaagct gccgttacgc gtgatatgac tattgtggca
     8821 ttgaccggct atgacggcgg cgaacttgca ggtttgttag ggccacaaga tgtggagatc
     8881 cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc atatgctgac ggtaaattgc
     8941 ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg atgtttaagg agaatacatg
     9001 aaggcattat cgccaatcgc agtccttatt tccgcgctgc tgttgcaagg ttgtgttgcc
     9061 gctgccgtag tgggtaccgc tgctgtgggt accaaagccg caactgaccc acgcagtgtc
     9121 ggcacccagg tggacgatgg taccctggaa gtgcgcgtga acagcgcatt gtcgaaagac
     9181 gaacagatta agaaagaagc ccgtattaat gtcacggcgt atcagggcaa agtgctgctg
     9241 gttgggcagt caccaaacgc tgaactttcg gctcgcgcca aacagattgc tatgggcgta
     9301 gacggtgcca acgaagtgta taacgagatt cgtcagggcc agccgattgg tctgggcgaa
     9361 gcgtctaacg atacgtggat caccaccaaa gtgcgttcgc agctcttaac cagcgacctg
     9421 gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct gatggggctg
     9481 gtgactgaac gtgaagcgaa agcggcggca gatattgcca gccgggtgag cggcgtaaag
     9541 cgggtaacca cagcgtttac gtttattaaa tagcagcccc ttgtaatgcc tgatgcgacg
     9601 cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta ggtcggataa ggcgtttacg
     9661 ccgcatccga catgttttcc ctcaaatcag aacaacagcg ccagcccgcc gacaatcaca
     9721 ccagacactg ccaccatcgc ccctgtcagc cataaggctt tcgctgggaa cgctttgcgc
     9781 agcataatca gtgacggcaa actcaccgcc gggagcgtca tcaacaatgc cagcgccgga
     9841 gcagttccca tacctgccag catcatcgtt tgtacaatcg gaatttctgc tgccgtggga
     9901 atgacaaaca agcatcctgc taccgccatc gccatcaccc acatcaggct gttatcgaca
     9961 gcaccatcgg catgggggaa taaccagacg cgagcggcac ccaacaccag tactgcaagg
    10021 atgtaaaccg ggatcgtact ccagaaaagc gtccatagcg ccctgcccca gcggctaaaa
    10081 aaaccgccct gtgcttccgg tatgtcaatt tcgaccggtg cctgcgtttg cggtgtttca
    10141 cgcacccatt tttgcaccag cgtcgcaatc agcaacacca tcaccagccc ggccaccaga
    10201 cgaatcgccg caaaacccca gccgaggaca aaccccataa acaccagcgt cgccgggttt
    10261 aacaccggat tgcccatcca gaatgccagc gcaccgccca tcgacacctg ttggcgacgc
    10321 attcccgccg cgaccggagc cgcacagcag gtacacatca tgcccggcag cgaaaacagc
    10381 gttcccagca gcgtgccgcg aaagcgcgat tgcccaagcg tacgcaacaa ccagtcacgc
    10441 gggatcagca cctgaatcaa cgaaccgagg atcaccccca gcactgccgc tttccatacc
    10501 gcgaggaaat agagcatcgc gtaatccaac gccgcctgcc acggattagc atccgcctgc
    10561 gcaaggatag atttaccgat actgtgggtt tcggcagcag taaacgcttt gccgtagtaa
    10621 ggttgccatt tcacatacca gagaccggca atgacaacga gaaagaaaag cgcgggtttc
    10681 caccactgaa tgggcgttgc cgcctgagat gaagactgac cagtcatagc attccccagg
    10741 aataagttat gataattgag cgcgtgaata ttacgctcac tatcaattct tgggaataat
    10801 tattcagctc ttttgcgtaa ttctgaagag cttaaaatcg tcacaccttc atgcccgggc
    10861 cgcatcgcct ctgccagcat gacccgcgcc acgtctctcg catcaatgga tttccagtta
    10921 ccgggtaata aacggaacag cggcgcaaaa agcgtttcgt tcatccgctg tttgctacga
    10981 tcgcccagta acatcgacgg gcgagcaatg gtcaatttcg gccagttctg ggcgattaat
    11041 gcttcttcca tctccccttt gacgcggtta tagaaaaacg gcgagtgggc attggcaccc
    11101 atagcactga ccaccagcat atgctgcgcg cccagtcgcc gcccggttaa tgcggtatcc
    11161 actaccagcg tgtaatcggc atgaataaac gcctctttgc tccccgcttc tcgccgcgtg
    11221 gtgccgagac aacaaaacac aatgtcgatg ggatcggtga cctgcgccag cgcgtcagtc
    11281 agttgcggat catggggatt aaacaccccg ggcatatcgc ccaacggacg tcgcgtcggc
    11341 gcggctatgg cgttaacttt cggttcgtta atcaacatcc gcagcaggtg accgcccacc
    11401 agccccgttg cgcctgtaat cagtacctga ctcatcttcg ctcctttaca gaattgtctg
    11461 ccttgcgctc cacggctcat gcaccaggct taatagacca tgaggtaatt atccccgatt
    11521 gtgggaaatt cgcctcatcc aatgcaacaa cgcggaggaa gcatgagtaa gaaaattgcc
    11581 gttttaatca ctgatgaatt tgaagattca gaatttactt cacccgcaga cgagttccgt
    11641 aaagccggac acgaagtgat taccattgaa aaacaagcgg gtaaaacggt gaaaggcaaa
    11701 aaaggagaag ccagcgtgac catcgataaa tccatcgatg aagtgacgcc agcggagttt
    11761 gatgccctgc tgctaccggg cggccattca ccggattatc tgcgtggcga caaccgtttt
    11821 gtcaccttta cccgtgattt tgtgaatagc ggcaaacctg ttttcgccat ctgtcacggt
    11881 ccgcagctgc tgatcagcgc cgatgtgatt cgggggcgca aactgaccgc ggttaaaccg
    11941 atcattattg atgttaaaaa cgcgggcgcg gaattttacg atcaggaagt cgtggtcgat
    12001 aaagatcagc tggttaccag ccggacaccg gacgatctgc cagcgtttaa ccgcgaggcg
    12061 ttacgcctgc tcggtgcctg agccgcgtag ccaaatcatt tttttaccaa agcccagcgt
    12121 gttgtcggtg aatttgattt catcgagccg gatttcccac accggggccg acagcattct
    12181 ggcaaccgga aagcgacgat tgtacgcctt gcgcgcgagg tcgctttcct caccttccag
    12241 cctgcggatc tcacctttaa actgcacgcc gcgaattaac gctaccgttt tcggctgacc
    12301 gtttaccgtt ccggcaaccg cagcctgcgg cccactcatc tgcgcgtggc gcgttttttc
    12361 ttccgtcaaa atgtagaagg cgactttctg cgcatcaaac agataaaaag cattagcgca
    12421 ccagagttcc ccttcctgct gcacacacca ggtgacaaca tgttgttttg ccaaccaacg
    12481 gctgatggtg atgagtgttt ccattgctgt tctcttttat actgtgggcc tgaccttaac
    12541 atgcgacgca gacgatgaca ccctggtttc tttacttgat ccgtaccgcc gacaataagc
    12601 tttataccgg gatcaccacg gatgtcgaac gccgctatca gcagcaccaa agcggcaaag
    12661 gggcgaaagc actgcgcggg aaaggagaac tgacgctggc gttttctgcg ccagtgggcg
    12721 atcgttcgct ggcgttacgg gcggaatatc gcgttaagca actgaccaaa cggcaaaaag
    12781 agcgtctggt agcggaaggc gcagggtttg cagagctgtt aagcagtctg caaaccccgg
    12841 agattaaaag cgattgaaat gctcgtgata ctcaaccaga ccagttacgc cattcaacgc
    12901 gtcatccgct aaacgatgta cctggaaggc gctttcagtg cctggccagc ggcaacgcag
    12961 gtcgtgatgt gccgccagtt caaagccaaa acggctgtac agcgccggat cgcccagcgt
    13021 caccactgcg gcatagccga actcattaag cgaatcgagt ccttcataga ccagttggcg
    13081 tgccagcccc tgtccacggt atttttcatc aaccgccaac ggtgccatgc cgacccattg
    13141 caggtcttcg ccctgcacat caaccggact aaatgccaca tagccaatga cctgaccttc
    13201 gtcatctgtc gccaccagcc ccagcgtcag aaagccatct tcacgcagat cgtgaaccag
    13261 cttcgcttcc gcatcacttt cgaatgagcg acgtagcagg gcatcaatac ccggcgcatc
    13321 aatgggaatt tctactcgaa ttagcatggt tcacctaccg atgtctgttt ggtttcaggc
    13381 gcggttttca ttcccgcctc aacaaaatcc gccagttgca gcagcatcat gcgtaaggct
    13441 ttcggcattt gctccagttc aatggcgtcc atcaggtttt tcacatacag ccccagctcc
    13501 gtatcgcctt caatcaccag ccgacgctgg aagaagagcg tatccggatc ttgtttacgc
    13561 gccgcaatca tcagcagatc gctggcgtcg gcactaaaac tcacatcagc ttgcgcgttc
    13621 tggctgacca ccagtttgcc attcaccacc gaggtaaacc attgcaggtc aatatcacgc
    13681 acatgaatac ttaaccagcg gccttcaaga aactccagct cgccatcatc cagcgcctga
    13741 cggaattgcc agcttaagac ctgctcaaga acctggcgtt ttagcgcaaa tggcgtcagt
    13801 tttaccggta cactcaacag agatggcccc aaatgcacaa tacgggaacg cagtttatcc
    13861 aacacgagtt ttactccctg tttcaacaat catcctattt tgccatatca gaaaaataac
    13921 atagcggtat aaatcaacaa ttccatatga aattgctgct accaccaata caactttaac
    13981 tgccttaaat caaaaattgt cgcagcaagg ttaactaaaa tcccagttcg ttaacatttt
    14041 tgcgttttga tagcgcaacc ttcaggaaaa attatggagc tgctctgccc tgccggaaat
    14101 ctcccggcgc ttaaggcggc catcgaaaac ggcgcagatg ctgtttatat cgggctaaaa
    14161 gatgatacca atgcccgtca cttcgccggc cttaacttta ccgagaaaaa attgcaggaa
    14221 gcggtgagtt ttgtccatca acatcgccgc aaacttcaca tcgcgattaa cacttttgcg
    14281 catccggacg gttacgcccg ttggcagcgt gccgtggata tggcggcgca gctgggtgcc
    14341 gacgcgctga tcctcgccga cctcgccatg ctagagtacg ccgccgagcg ttacccgcat
    14401 attgagcgtc atgtgtcagt gcaggcttcg gcgaccaatg aagaggcgat taacttttat
    14461 catcgccatt ttgacgttgc tcgcgtggtg ctgccgcgcg tgttgtcgat tcatcaggtg
    14521 aaacaactgg cacgggtcac acctgtacca ctggaagtct ttgctttcgg cagcctgtgc
    14581 attatgtcgg aaggtcgttg ctatctgtcg tcgtatctga cgggtgagtc gcccaacacc
    14641 gtaggcgcgt gttctccggc ccgtttcgtg cgctggcaac aaacgccgca ggggctggaa
    14701 tcccgcctga acgaagtgct gatcgaccgt tatcaggacg gcgaaaacgc aggttatccg
    14761 acgctatgta aagggcgtta tctggtggac ggcgagcgct atcacgcgct ggaagaacca
    14821 accagtctca ataccctgga actgctgccg gagttaatgg cggcgaatat tgcttcggtg
    14881 aaaattgaag gccgccagcg tagcccggcg tatgtcagcc aggtggcgaa agtctggcgt
    14941 caggctatcg accgttgtaa ggccgatccg caaaacttca taccgcaaag cgcgtggatg
    15001 gagacgctcg ggtcgatgtc cgaaggcacg cagaccaccc ttggcgcgta tcaccgtaaa
    15061 tggcagtgag aagagcaatg aaatattcct tagggccagt gctgtggtac tggccaaaag
    15121 agacgctgga agatttttat cagcaggccg ccaccagcag cgccgacgtg atttatcttg
    15181 gtgaagcggt atgcagcaag cgccgggcaa ccaaagttgg cgactggctg gagatggcaa
    15241 aatcgctcgc cggaagtggc aagcagattg tgctctccac gctggcgctg gtgcaggcat
    15301 cctctgaact gggcgaactg aaacgctatg ttgagaacgg tgagtttctg attgaagcca
    15361 gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc gccgggcacg
    15421 cgctgaactg ctacaacgcg gtgacgctga aaatattgct caaacagggc atgatgcgct
    15481 ggtgtatgcc ggtggagctt tcccgcgact ggctggtgaa tctgcttaat cagtgcgatg
    15541 agctgggcat tcgcaaccag tttgaagtgg aagtcctgag ctacggtcat ctaccgctgg
    15601 cctactccgc ccgctgcttt accgcgcgtt cggaagaccg cccgaaagat gagtgtgaaa
    15661 cctgctgcat taagtatccg aacgggcgca acgtgctgtc gcaggaaaac caacaagtgt
    15721 ttgtactcaa tggcattcag accatgagcg gctacgttta caacctcggt aacgagctgg
    15781 catccatgca ggggctggtt gatgtggtcc gcctgtcacc gcagggtact gacactttcg
    15841 cgatgctcga cgccttccgc gctaatgaaa atggcgctgc gccactaccg ttgacggcaa
    15901 acagcgactg taacggctac tggcggcggc tggccgggct ggagctgcaa gcctaagtaa
    15961 atatgctcgt catacttcaa gttgcatgtg ctgcatctgc attcgttcac cccagtcact
    16021 tacttatgta agctcctggg gattcactct cttgtcgcct tcctgcaact cgaattattt
    16081 agagcacagc tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa
    16141 tcggtaacaa agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg
    16201 gcccccattc ccgaaggttc ttcagcgcga gaagcattct cccactctct cgatctcgcc
    16261 cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgact
    16321 ggcattgcca gtgctgccac gtcggtattg attggctatc tggcggcgaa taccaccacg
    16381 ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt cattgccgaa
    16441 cagttcggca cgctcaatac actctatccg gggcgaatcg atttggggct gggtcgtgcg
    16501 ccaggtagtg accaacggac aatgatggcg ctgcgtcgcc atatgagcgg cgatattgat
    16561 aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg
    16621 catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc
    16681 ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt tcgcgtttgc ctcacacttc
    16741 gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa accgtcggca
    16801 cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga tagcaaccgc
    16861 gacgctgagt ttctgtttac ctcaatgcag caagcctttg tgaagttgcg ccgtggcgaa
    16921 accgggcaac tgccgccacc gattcaaaat atggatcagt tctggtcgcc gtccgaacag
    16981 tatggtgtgc agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa agtgcgtcat
    17041 ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggtcaa cgggcagatt
    17101 ttcgaccacc aggcgcggct gcattcgttt gagctggcga tggatgttaa ggaagagttg
    17161 ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcc
//
LOCUS       DAZFKM010000104        16807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFKM010000104 DAZFKM010000000
VERSION     DAZFKM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(105..2096)
                     /gene="tkt"
                     /locus_tag="ACHKAY_003585"
     CDS             complement(105..2096)
                     /gene="tkt"
                     /locus_tag="ACHKAY_003585"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO5702197.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2374..3132
                     /gene="loiP"
                     /locus_tag="ACHKAY_003586"
                     /gene_synonym="yggG"
     CDS             2374..3132
                     /gene="loiP"
                     /locus_tag="ACHKAY_003586"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO5702198.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3188..3931)
                     /locus_tag="ACHKAY_003587"
     CDS             complement(3188..3931)
                     /locus_tag="ACHKAY_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO5702199.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3918..5027)
                     /gene="dgaE"
                     /locus_tag="ACHKAY_003588"
     CDS             complement(3918..5027)
                     /gene="dgaE"
                     /locus_tag="ACHKAY_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO5702200.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5031..5891)
                     /locus_tag="ACHKAY_003589"
     CDS             complement(5031..5891)
                     /locus_tag="ACHKAY_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO5702201.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5888..6637)
                     /locus_tag="ACHKAY_003590"
     CDS             complement(5888..6637)
                     /locus_tag="ACHKAY_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO5702202.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6663..7148)
                     /locus_tag="ACHKAY_003591"
     CDS             complement(6663..7148)
                     /locus_tag="ACHKAY_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5702203.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7159..7587)
                     /locus_tag="ACHKAY_003592"
     CDS             complement(7159..7587)
                     /locus_tag="ACHKAY_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO5702204.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7706..10504)
                     /gene="dagR"
                     /locus_tag="ACHKAY_003593"
     CDS             complement(7706..10504)
                     /gene="dagR"
                     /locus_tag="ACHKAY_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO5702205.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10763..11683)
                     /gene="speB"
                     /locus_tag="ACHKAY_003594"
     CDS             complement(10763..11683)
                     /gene="speB"
                     /locus_tag="ACHKAY_003594"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO5702206.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11819..12550)
                     /locus_tag="ACHKAY_003595"
     CDS             complement(11819..12550)
                     /locus_tag="ACHKAY_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702207.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12696..14672)
                     /gene="speA"
                     /locus_tag="ACHKAY_003596"
     CDS             complement(12696..14672)
                     /gene="speA"
                     /locus_tag="ACHKAY_003596"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO5702208.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14681..14812)
                     /gene="yqgB"
                     /locus_tag="ACHKAY_003597"
     CDS             complement(14681..14812)
                     /gene="yqgB"
                     /locus_tag="ACHKAY_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO5702209.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14906..15049)
                     /gene="yqgG"
                     /locus_tag="ACHKAY_003598"
     CDS             complement(14906..15049)
                     /gene="yqgG"
                     /locus_tag="ACHKAY_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO5702210.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15160..15411)
                     /gene="yqgD"
                     /locus_tag="ACHKAY_003599"
     CDS             complement(15160..15411)
                     /gene="yqgD"
                     /locus_tag="ACHKAY_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO5702211.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15467..16621
                     /gene="metK"
                     /locus_tag="ACHKAY_003600"
     CDS             15467..16621
                     /gene="metK"
                     /locus_tag="ACHKAY_003600"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO5702212.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         4106 a         4658 c         4210 g         3833 t
ORIGIN      
        1 atatttgtga tctacaacac gctttatcta ttgcttgtcc gcaaacggac atatcaaggt
       61 aataaaaaag gtcgccgaag cgaccttttt acccgaaatg ctaattacag cagttctttt
      121 gctttcgcaa caacgttatc aacagtgaag ccgaactctt caaacagcag ctctgccgga
      181 gcagattcac cgaaggtggt cataccgacg atagcaccgt tcaggccaac atacttgtac
      241 cagtagtcag caatacccgc ttctacagca acgcgtgcag taaccgcttt cggcagtacg
      301 gattcacggt aagcagcatc ctgcttgtca aatgcgtcgg tagacggcat ggacaccacg
      361 cgcgctttca cgccttcggc agtcagtttt tcgtaggcag caacagccag ttcaacttct
      421 gaaccggtag cgatgaaaat cagttccggc tgaccggcgc agtctttcag cacataacca
      481 ccgcgcgcga tgtttgccag ttgctcttca gttcgttcct gctgcgccag gttctgacgg
      541 gagaggatca gtgcggtcgg gccgtcctga cgctcaacac cgtatttcca cgcgaccgcg
      601 gattcaacct ggtcacacgg acgccatgta gacatgttcg gggttacgcg cagagaagcg
      661 acctgctcta ccggctggtg agtcgggcca tcttcgccca gaccgatgga gtcgtgggtg
      721 taaaccatca cctgacgctg tttcatcagc gcagccatac gtacggcgtt acgtgcgtat
      781 tccacgaaca tcaggaaggt ggaggtgtac ggcaggaaac caccgtgcag ggagataccg
      841 ttagcaatcg cggtcatacc gaactcgcga acaccgtagt ggatgtagtt acccgcagca
      901 tcttcgttga ttgctttaga accagaccac agggtcaggt tagacggtgc caggtcagca
      961 gaaccgccga ggaattccgg caacagcgga ccgaaagctt cgatagcatt ctgagacgct
     1021 ttacggctgg cgattttcgc cgggttagcc tgcagtttag caatgaactc ttttgctttc
     1081 gcgtcgaagt cagacggcat ttcgcctttc atacggcggg taaattcagc ggcttcctgc
     1141 ggataagctt tcgcgtaagc agcgaatttc tcgttccatg cggattcttt cgcctggcct
     1201 gcttctttcg catcccactg agcatagatt tcagacggga tttcgaacgg tgcatatttc
     1261 cagcccagct gttcgcgggt cagggcaatt tcagcgtcgc ccagcggcgc accgtgggag
     1321 tcgtgagtac cggctttgtt cggggaaccg aaaccgatga tggttttgca catcagcagg
     1381 gacggtttgt cagtcactgc gcgcgcttct tctactgcgc gtttgatgga tgccgcgtca
     1441 tgaccgtcga tgtcgcgaat aacgtgccag ccgtaagctt cgaaacgcat tgcggtgtcg
     1501 tcggtgaacc agccttcaac gtgaccatca atagagatac cgttgtcatc gtagaacgca
     1561 atcagtttac ccagcttcag cgtacccgcc agagagcaaa cttcgtggga gatgccttcc
     1621 atcatgcagc cgtcgcccat gaaggcgtag gtgtagtggt cgacaatgtc gtggcccgga
     1681 cggttaaact gcgccgccag cgttttttct gcaatcgcca tacctactgc gttggcaata
     1741 ccctgcccca gcggaccggt ggtggtttcc acaccagcgg tgtaacccac ttccgggtgg
     1801 cccggagttt tagagtgcag ctgacggaag tttttcagtt cttccatcgg cagatcgtaa
     1861 ccggtgaggt gcagcaggct gtagatcagc atggagccgt ggccgttgga cagcacgaag
     1921 cggtcacggt cagcccagga cggattctgc gggttgtgtt tcaggaaatc acgccacagg
     1981 acttcggcaa tgtcagccat acccataggg gcacccgggt gaccggattt ggctttctgt
     2041 actgcgtcca tgctcagcgc acgaatagca ttggcaagct ctttacgtga ggacattttg
     2101 actccagatc ggatgatgaa gggcacgccc ttaacgactt gacgacagcg cgttttgggc
     2161 tacgccggaa aatttgccaa caatttaccg caagccgcgc gtcatgtaca tggaacatcc
     2221 ttttgccgct tcagaaatct ctggatcatg ctcgcatgtt gcgcaatcta ctcgcccgtc
     2281 cgctgcgctt ttccttatac tgagactgag cgtcgattca cctgcaaacg gcgcattttt
     2341 agaataatcc tgaccttgtg cggaagagaa aacatgaaaa ttcgcgcctt attggtagca
     2401 atgagcgtgg caacggtact gactggttgc cagaatatgg actccaacgg actgctctca
     2461 tcaggagcgg aagcttttca ggcttacagt ttgagtgatg cgcaggtgaa aaccctgagc
     2521 gatcaggcat gtcaggagat ggacagcaag gcgacgattg cgccagccaa tagcgaatac
     2581 gctaaacgtc tgacaactat tgccaatgcg ctaggcaaca atatcaacgg tcagccggtg
     2641 aattacaaag tgtatatggc gaaggatgtg aacgcctttg caatggctaa cggctgtatc
     2701 cgcgtctata gcgggctgat ggatatgatg acggataacg aagtcgaagc ggtgatcggt
     2761 cacgaaatgg ggcacgtggc gttaggccat gtgaaaaaag gaatgcaggt ggcacttggt
     2821 acaaatgccg tgcgagtagc tgcggcctct gcgggcggga ttgtcggaag tttatctcaa
     2881 tcacaacttg gtgatctggg cgagaaatta gtcaattcgc aattctccca gcgccaggaa
     2941 gcagaagccg atgattattc ttacgatctt ctgcgccaac gcggcatcag cccggcaggt
     3001 cttgccacca gctttgaaaa actggcaaaa ctggaagaag gtcgccaaag ctcaatgttt
     3061 gacgaccatc ctgcatccgc cgaacgcgcc cagcatattc gcgatcgtat gagcgcggat
     3121 ggggttaagt aagtcagaca taccgatcta caacataatt gtaggtcggt aacgcctgaa
     3181 tccccgatca gagtagtgat ttcactttat ttagcaaaac ggctacatca tccggtcggg
     3241 tatatcccgt atcagggtct ataatcgagc tgtaaacatg tggcataatg cgcggcaccc
     3301 ccgcctccag acaagttttc agaatcacac tgaagttatc caaatcaatc ccgccagtcg
     3361 gctcaatcag cgtcatacca ttgcgcgccg ccgtggtcgc cagcgcgtat agctccggaa
     3421 gcgacctctc gccgcccatc ggaaagaact tcgccgcgtg tgcgcccata tctagcatca
     3481 ttcgcaccgc cgtatcgcag gagacgcggg caggcgtacc ctggctgctg ctaacgccgg
     3541 tggaaatcag cacttcgccc ggcgtccccg tcgggctaac cagcgcgttg acatgggtct
     3601 gctcaccgcc cgtagccgca agcgcgcctg ccgcgaaacc acaaccggta aaggtctggt
     3661 taacgtgtgc tggatgaatc tgcgccgcaa tcatcgcagc tttgtaatac tgcgccgggt
     3721 cacccgcccc cagacctact gaaatggacg gtatttccgc catccaacgt ttaacttcct
     3781 ggatcccctc atccaccgag gagaactgcg cggaaagcac gccaatcact gcgtggcctt
     3841 cggcggcgtc aaagatttca cgggcattgg cgatatcttt cgccagcaca ttaattgcca
     3901 cgcgattacg atagaaatta atcttttgca tgatctgcaa tctccttcag tcgcgccacg
     3961 ataagtgcca tttccccttc cgccaccgta cgcggatcaa ggctaaaaac gccctggtgg
     4021 aggttatacc gccgtgcata aatcgcgata tcgccaccac gcagttgcgc ttcgacgacg
     4081 cgagcgtcaa ctcccaattc ctgcgcattt actcgaatac gaatacgcca gattgcacgc
     4141 ccggcttcat cctgctcaat atcagccgtt aacccgtgaa tcgcagaaat cgcttcaacc
     4201 actggctgga gctgttccgc ggtgatgact gcctgcccct ggtgataatt ctccagcgcg
     4261 taaaccagcc caaccatgtt ctccttgccg attttcatcg ccctggcaat gccgtgatgc
     4321 tgcgctttgc aggctgcaat ccagattttt ttaccagtga taaacccgga ggtcggcgcg
     4381 ttaaaggctt ttgcgccgct gtagaccacc atatccgctc cgctcaccac ccaggcacgt
     4441 aaatcctctt cggcggcggc atcgacaatc agcggcaggt tgtacatctg cgcgacatgt
     4501 gcgaagtcct cgatgttcaa cattcccttc tgcacgcagt gatgcgactt cacatacagc
     4561 agcgcggcgg tcgcgtcggt aatggcactc tccagctgcc agagagccgc caggttactt
     4621 gaacccacct ccacaacgcg cccgccacct aagcggatcg cgctgaggat cgatgcaccg
     4681 taatcgacgt tatggccgcg cagcatcacc acttcattgg ccatgccgga gctatccggc
     4741 atgagcgcca cccgggcctt gtcgccgcgt gtaatggcgg cggcgacagc gatggcgatc
     4801 cctgctgagg cacaggaggt aacgtagcta tcctccgccc cggtaaagcg agagaccagc
     4861 tcgccggttt tgtccaccag ctggtcaatc tccacgaaag aggatgccgc gcgcgcggtg
     4921 gcctgcatca cctccggcgc gacgctggag acgccgagaa tcgtcatctt gccgcaggcg
     4981 ttaatcacct gtttcagtcc cagttgttga tagatattct gcgtcatggc ttacagcact
     5041 cccagcagcg agcagaccac gctcaacgcc acaatcgaca gcagaatcgt ggtgtaacgc
     5101 ggaccctttt tcaccaaata gaagtagatg gcgaacactg ccgccagcgg cagcaggccg
     5161 ggcgcgatgg agtccagaat ctgctgtacc accacttcag agcctttcag tgcgctaatt
     5221 tttaaaggtg aggttatctt gacaaagctc gcggatagcg cgcccatcat gataagcccc
     5281 agtacgttag cgccgtagat aagctccttg atgcgccctc cctgcagcag actgataatc
     5341 gaatcgcgcc ccagcgtata gcccttgtgc accatgccgt agctgattgc cagcgtaatc
     5401 gccgggtaga gaatcagcgg caaaataccg cccatcgcgc tgccattggc ggcgaacggg
     5461 ataaaaatag caatcaacag cggcatcacc gccgcccaga tgatggagtc gcccatcccg
     5521 gccagcggcc ccatcaggcc ggttttaatc ccggtgatag aggcatcgtt aatcgcctcg
     5581 ccgcgggtct tttgctcttc catggcgatg gagatcccct ggatcaccgc gccaaacgtc
     5641 tgctctgagt tgaagaagtt cagatggcgt tttaacgcct ccacctgctc ctccttttgc
     5701 ggatagagct ttttgataat cggcgtcatc gaggcgcaga aaatcaggct ctgtaaacgc
     5761 tcataggagc tggaaacttc cgcgcccagc cagtagataa accacgcttt ggtgatatcc
     5821 gctttggtca gcgccccgga ctcccgcgcg cgctcaacca gttcatgctg cattacgtcg
     5881 tcgctcatta tgccgctcct tcatttttcg ccaggccttt aatcaggaag gccacgcagg
     5941 tgccgaaaat cgccatcgcc atgatgtcca ccttgaggta cagcaccgcg aagaatccgc
     6001 cgataaacca cggcagcagg ctctttttac caatcaccat aatggtgatg gcgaagccca
     6061 gcgccgggag aatcccgccc atgatttcaa acgagtgggt cagccagtgc ggcatcagct
     6121 tgaggaaact ttccactacg ttctggccga agtagttggc ggcgaacacc actgggaaac
     6181 gtagtaccag ccccagcagc gccgggtaga gaaaggcgca gcgcataatg gctgacatat
     6241 tggccgtttc cgcgtgttta tcggccatat gcacccaggc cgcatttagc gtgcgacgca
     6301 actggtcgag gaatacgccg ataacgccaa atggaatcgc cagtgcaatc gccaggttcg
     6361 ggtccatatt ggccttaacg gcgataggaa tggcgataca ggccgccagc gccgggtctg
     6421 acggcacatt gccgcctggc gttgaggtca cacccagata gaccaactgc atccccgcgc
     6481 cgataatcat cgccgcaggc atattgccga gcagcaggcc gacaaagacc gccaccacca
     6541 ctggctgtaa cagcatggcg gagaaggtat agccaaggcg caaccgggca aaccagtaat
     6601 acagccccat caggcttgca aaaagtaagg tatccataat ttgttatccc atgttcagag
     6661 ggtcagaatt ttttcaggat atcgtccagc gactgcggtt tatcttccgg gatggtctgg
     6721 aaaacagcct taatgccgcg acttttcaaa tcgttaagaa tgccaacgtc tttttcatcc
     6781 agcgttatgt tctggaacac cgctttacgg tttggcccgc cgcctaaacc gccaacctga
     6841 atatcagcca cgtcaaagcc ctgctgcacc gcttcctgaa tcgccgccag cgacgggaac
     6901 agcaccagca cgttgccgtc gcccagctgg ttctctttcc acgcggccgc gaagctctgg
     6961 ttgctaaagc agtccacttt gatattcggc ggcgcggcca tcaggtagat atttttcata
     7021 aacggatcgg cgtccagctc gtcgctgacc accgcaatac ggttggcctg agactgccct
     7081 acccacttcg tcaccacctg tccgtggata agacggctgt caatgcgaca taacacgatg
     7141 tttgccatac gattttcctt actgttgtaa ttgacgaata tgggagacga cgtccaggca
     7201 actgttgcgc ccagcttcca ccagatcggc cacgcaggcg gtcagtgggc catgttcgcg
     7261 cttgtccagc gcctccagca gcagcgaggc attaagcccg gagatcaccg ccaccggata
     7321 atcggcgctc agccgcgccg ccacgttaga ggtggtgccg ccgataaaat cggtcacgat
     7381 aagcgaacct tccggcatgg ttttcaccac ggcttcgacg cgctggtaaa actcgcccag
     7441 cgtatcgacg ggcatcagcg cgatttccgt cacgccttcg atttcgccca tcaccatgcg
     7501 cagactgttg cagagctgca gcccccagcc gccgtgagtt agcagcagaa tttcaggtaa
     7561 cggatgaata ggtacagttg tggtcaaaat agcctccttt gctccagtgt ttacatttca
     7621 tcaggagcaa aggacgtgcc agaatttgtg tcacgcacag aggaaatgag attggatggg
     7681 gcgttaagcc ccgtaaacca ggggatcagc tgtagagcag ttcgtagata taaaaatatt
     7741 ctgcgtccga taaacggata gcgtaagcct cttcaatcgg caaaaaggag gatttgatga
     7801 cgctaaaggc acgagcgtcc agatcaagcc ggttttctag cgccatctgt aacggtttgc
     7861 ggttaatcac gatacgctcg accatgcagc agcagtggat cagaaaacgt agtgtcacct
     7921 ggcggctcgg tttcagggag agcgtgttgg ttaagtagtt cagaacatcc tcgatctctt
     7981 tcagaattcg ctgcgggttg agcaccgaaa tatggttgat gatgctctcc atggtcagcg
     8041 cgctgataaa gcgcatggcg ctgcgctcca tctccagtcg gcgctcgctg cttgagagat
     8101 ccggcgtcag caggctgagc accagctccg gcccttgctc ggaaaacagt tcctccagcg
     8161 agataaacgg tatatccggc agtcccggct ggaaggtgcc gacgatccct gccagccgtt
     8221 cactggcatt aagcgcctgc tgaatacgct ccaggctgcg cacctcgtta tagtcgagaa
     8281 tcaccatacg cgtgtcctgc gacatcagct cgccaaagct ctcctccagc acctttttga
     8341 tcttttccgc cgtccccatt ccggttatac atgagataac cagcactttg ccaccgctct
     8401 cttgctgcgg cgtgcagagc cgacaaggga tatttttact ctgcataagc acagccaatt
     8461 gcgatagatc gccagtttcg tagcttaaat ccagcccaac ctccagcagg ctggtcagag
     8521 tgatatttgg caacaacaga acgtcaatct ggaacagctt gctgatggtg ctgccaaaat
     8581 ggaccagcga tccgatatcc accatcagta tcagccgctg ccagcaccgg tcctgaatca
     8641 tccgggtcag ggtttccagg gtgtcatgca ccgactgctc aaatggcata tcaattgcgt
     8701 agaacagatc gcgctccagc acccggttca catattgcgc ctggctcgtt gcggtggtcg
     8761 caccgtgagc gattagaatc acgccgcaat ccgggctggt gtcgatgcgc tggcggtagt
     8821 ggcgacactc tttcaggaac aggcacagcc agaccacctc cgtcgccggg cactgaatat
     8881 gcaacaactc attgattttg cggcacagaa gcgtggcatt gtcatactca tctttacagc
     8941 gatccagaat caggctggat gaatagagtt gaggtataag ccctcgccgc acatagccaa
     9001 tcagcgcaag aaagtgttta cgtaacgggt tcgccaggtt ttctggcaat gtaaatccta
     9061 atacctgctc cacgtaaccg atcagcagcg tcacacgctc ctcaatttga tcgccatagc
     9121 gcggtggatg cgccgcacta tggcggctat aaagaccata ttcaaaaatc gaactgagtt
     9181 tgtttttcag gatagccagc gtttctgcgg gcggcacgtt actgttgcgc agattgacat
     9241 attcacgagt caggaaactg taaaacaggt cgctctcttc tatttccgcg ctggtcgcca
     9301 acgaattttt taacgcgggc aacgtacgcg catccacgtt cagacgatct ttaccgccaa
     9361 acaacgcatc gaccagcaga cgctgctctg gcgtggtatt aaacgacatc tccgccagtc
     9421 gtttatctaa ctctagcgta tcattacgct cggtcattcc cgacgcccac gcctgcgcgc
     9481 acaagaactg aatatcactt ttaagctggc cgatattgcc ttccaggggt ttacttaaca
     9541 accaatgcaa gagcgtttta tcaaggctta tggtacgttc aattttacga ctttcacgct
     9601 gaaagaagct tacaatcaat tcgagctgct cctcgaccga acgctggcga atacccggta
     9661 agtcaataca tacctggata cgccgctgga aggtacgcaa tagtgccgag ttgaccgact
     9721 ctgtagtggc gcaaataagg cgcaccgaaa ttgaatatgt tggcccactt gaaccaagtg
     9781 ggcggtattc acctttatcg agaatagaaa agagcttctc ttgtccttcg tacgggaggc
     9841 gatgcacttc atccagaagt aaataacctc cgtcagcttg ctccaccaat cccgttttat
     9901 tctcacttgc cccggtaaat gcccccttat ggtgaccaaa cagatgtgag gaaagcaact
     9961 ccggattatg agcatattcg gcacagttaa agtaaaccaa cggaggtggc gaacctatgg
    10021 tgtgcttgca agcgaagcaa tgcattaact cagcaaagaa tgttttgcca acaccagatg
    10081 caccggttaa cagcacatgt agtccgtggg gataaagcac cgctgcgcgg cctttttcta
    10141 ccgcatcacg gagactacgg tcgtatccga taagtccgga aaacggatca tcatctgtat
    10201 atttttcttt ctttggcaat aaactagcca ctgaatgaac tatgcattcg caatcatcca
    10261 gttttcgatt aatgagcaat tctagtgcgt gacgatgtag aaaaaaaaca ggacgtccct
    10321 gactttttac caccaggcct tcattccata attgattaag atctttacta accgagttgc
    10381 gtgccagccc aaggttaaaa ccaatcgcct cagcagtaaa tgctttctcc ttcgctaaat
    10441 cagacagttc cagcccatac gtcagccgct ccagctcctt gtgaataaca tcaatccgtc
    10501 tcatttcttt accgcttaaa aaacaatagt tgttcagaac atacactatt tgtaaacgta
    10561 agaatgagaa ctatcgggaa gaattaatgt tccttgtggc aatctggttt ctgaatattc
    10621 gtgttttaaa tattacttat ggtctgaaac aaccaggcag gtgaacagag ctaagaaaaa
    10681 gtagcgtaga aatataaaaa catatatcgc actgatgaat gtttcccggc atgactatgc
    10741 acaccgggaa atatttagta aattactcgc cctttttcgc cgcctgaata tacagcatct
    10801 ccagcgccag cgtcgccgca gccagagcag tgatttccga ctgatcgtat gccggagcca
    10861 cttccactac gtccatccca acgatgttga gatctttcag gccgcgtacc agtttaatgg
    10921 cgcgatcgga ggtcagaccg ccaattaccg gcgtaccggt tcccggcgca aatgcaggat
    10981 ccaggcagtc gatatcaaag gtcaggtaaa ccggcatatc acccacaatt tgtttcacct
    11041 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
    11101 cgttgtcttt atcaaattcg gtacgaatac caatctgcac ggaatgattc ggatcgatca
    11161 gaccttcttt cggcgcggta tagaacatgg tgccgtggtc aaattcacaa ccgttcgcgt
    11221 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
    11281 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggagagcata cgcttaccgg
    11341 cagccagcag cttctcggcg tgcgcctgca gcttctcgct catctcacgg gcatcgccga
    11401 aagcatatac cagatcaccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
    11461 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggata gctgccggac
    11521 cgtggcgtcc ccccgcacga ccagaagtgg ccatatcgaa cggcacacca gtaatcaccc
    11581 agtcagcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
    11641 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
    11701 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
    11761 cgaaacacaa gtttgtgcta attatcgcct taaatgggcg aggcatcaag cgaggttgct
    11821 atttccgaca cggtggcggc gcatagccat tgttatctgc cggaaaggct tgtccagagc
    11881 acggcggcag cgcgttttgc gcctggcctt gtaattcaaa ggcggcaaag cctattacac
    11941 cggtattacg cacatcgcct tgcgccgaat tggctgcgta ggaatcagca acgttggcga
    12001 actgaaaagc cgcttcggta tgtttatctt tgcggaatcc tttaatcgct aaagaatctc
    12061 cggcattcac aatatagccg ttgttattca gcgaaccttg cttgccgttc agcacatcca
    12121 ggccatcaac agtagcaacg atctcgtagt tggtgttacg actgtaatta cggacatata
    12181 attgataatt cattccgact ctggcatgga caatatagcg tggttcatta ttggatgaat
    12241 cataaacacg cgtaataggt agagaattaa aattaccatc gcgaatagca tattccagtt
    12301 cgttaatacg aatgctataa acgcggtcat agccggaagg aacattagtc gaataactaa
    12361 taacgatcat ctctgccgga tcgcgccagg ctcgctcagc agatacgccc tgaaccgatg
    12421 agtgcacatc cccaccccag gcagtgccga cattgcccga tgcactgcta ttatactgcg
    12481 cattactgga acaaccggcc agcagcacga ttaatgccac caacgcgcta cgtactttcc
    12541 attttttcat aaccgttccc tctgcattta attaatgcag cgattgtagg gagattattt
    12601 aataagaaaa gcagcgagat tatttttaag aatatataaa aagaaagcgg gccgatttac
    12661 gtaaataaat cgacccgcca gcggaataat cttaattatt catcttcaag ataggtataa
    12721 ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg
    12781 gttttcttca cttgatcgcg gaactgggtt aacagcgttt tcggatcgag ctgtacatat
    12841 tgcagcatgt ccgccacggt atcgccttcg tcagacagtt ctacttctac gctaccgtca
    12901 gggaacacga acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat attgccaagg
    12961 atctcctgat atgcgccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac
    13021 tccggcattg gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg
    13081 tcagagtcac aggtaatatc cagcagcact gcgcgacgtt ccggcacttg atccagccct
    13141 tccagcggca gaaccgggaa caactggtcg atcccccatg catccggcat tgactggaac
    13201 agcgagaaat tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga
    13261 cgatgcgcac ggttttgcgg atccagctgc ttttgcactt catggcacat gctcagatag
    13321 agttgttctg cccatgcacg ttcttgcagg ctaaaggtgc cggaagagta gccgatatga
    13381 atgtcgtgca gatccatctg gctgtcgtgt aaccattcac gcagagaacg gcgagttccc
    13441 ggttcgtgca tttcctgcca ggtttcccac atgctttgca gcgcgcgcgg cgcatcttct
    13501 gcaggcgcgg tcggcaccgt gtattcgtta cgttccacgc cgatgatatt agataccagc
    13561 acggtgtgat gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg atgcggcaga
    13621 ccgttttctt cacacgcatc accaatcgcc cagatgatgt tgttggcata ttcattgagg
    13681 ccgtagttca ccgaacagtc ggactgcgaa cgggtgcctt catagtccac gcccagaccg
    13741 ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc
    13801 gcggattcac gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg
    13861 aagtgcagca gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aacaagttgc
    13921 agtacctgag tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta
    13981 cccgagccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt
    14041 tctgcttcat ccagcacaat ggcgatttct gacatcttct caatgaccag gtagaccttg
    14101 tggcccatct tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg
    14161 cagacgatga cgctacgggt catgccagca tgtgccagca ccgccatcaa ctctgctttc
    14221 gagcctgctt ccagtcccag cggttcgccc gaatgaatca gggactcaat cacgcggcgg
    14281 tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc
    14341 cgcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa
    14401 cagaacagtg caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga
    14461 tcgacgcgag cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta
    14521 acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct
    14581 tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac
    14641 gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg
    14701 taaaacagtt aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat
    14761 tttccagcag tgtatgctga cgctccgact gcgcaaccgg tttctttttc ataacattat
    14821 taagcacata accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt
    14881 ttacaagagc gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag
    14941 cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg
    15001 aatcttcacc agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc
    15061 acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct
    15121 ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt
    15181 ataccccaca atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg
    15241 aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg
    15301 ggtgaagaat tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact
    15361 cagactgcca gtgtttttaa cttgcagagt cgtggtagga tccgctacca cagaaaatcc
    15421 acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct
    15481 ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatttctga
    15541 tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta
    15601 cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc actagcgcct gggtagacat
    15661 cgaagagatc acccgtaaca ccgttcgcga aattggctat gtacattccg acatgggctt
    15721 tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca
    15781 gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg
    15841 ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct
    15901 ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc
    15961 gaaaagccag gtgactttcc agtatgacga cggcaaaatc gttggtatcg atgctgtcgt
    16021 gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga
    16081 agagatcatc aagccgattc tgcccgctga atggctgact tctgccacca aattcttcat
    16141 caacccgacc ggtcgttttg ttatcggtgg cccgatgggt gactgcggtc tgactggtcg
    16201 taaaattatc gttgatacct acggcggcat ggcacgtcac ggtggcggag cattctctgg
    16261 taaagatcca tcaaaagtgg accgttccgc tgcgtacgca gcacgttatg ttgcaaaaaa
    16321 catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt
    16381 ggctgaaccg acctccatca tggtagaaac tttcggtacc gagaaagtgc cttctgaaca
    16441 actgactctg ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct
    16501 ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca
    16561 tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta
    16621 atctttcttc acctgctttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc
    16681 gcggtggata ggcattcacg ccgcatccgg caaaaagctg cccgcacaat aacatcattc
    16741 ttcctcaaca cgttttatcg caaattatca tcactactga aaccgattac accaataaca
    16801 ccagcca
//
LOCUS       DAZFKM010000105        16776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFKM010000105 DAZFKM010000000
VERSION     DAZFKM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..178
                     /locus_tag="ACHKAY_003601"
     CDS             <1..178
                     /locus_tag="ACHKAY_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702213.1"
                     /translation="EDSSSEELLSDNTLEKDENSPDNGFEVGEYNTHEAYNSENQYFT
                     REDYTYNYDLLNAI"
     gene            complement(573..949)
                     /gene="rnpB"
                     /locus_tag="ACHKAY_003602"
     ncRNA           complement(573..949)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKAY_003602"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(982..2127)
                     /gene="garK"
                     /locus_tag="ACHKAY_003603"
     CDS             complement(982..2127)
                     /gene="garK"
                     /locus_tag="ACHKAY_003603"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO5702214.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGVAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2224..3114)
                     /gene="garR"
                     /locus_tag="ACHKAY_003604"
     CDS             complement(2224..3114)
                     /gene="garR"
                     /locus_tag="ACHKAY_003604"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO5702215.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3144..3914)
                     /gene="garL"
                     /locus_tag="ACHKAY_003605"
     CDS             complement(3144..3914)
                     /gene="garL"
                     /locus_tag="ACHKAY_003605"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO5702216.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3930..5264)
                     /gene="garP"
                     /locus_tag="ACHKAY_003606"
     CDS             complement(3930..5264)
                     /gene="garP"
                     /locus_tag="ACHKAY_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO5702217.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5639..7210
                     /gene="garD"
                     /locus_tag="ACHKAY_003607"
     CDS             5639..7210
                     /gene="garD"
                     /locus_tag="ACHKAY_003607"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO5702218.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7359..7694
                     /gene="prlF"
                     /locus_tag="ACHKAY_003608"
     CDS             7359..7694
                     /gene="prlF"
                     /locus_tag="ACHKAY_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO5702219.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7694..8158
                     /gene="yhaV"
                     /locus_tag="ACHKAY_003609"
     CDS             7694..8158
                     /gene="yhaV"
                     /locus_tag="ACHKAY_003609"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO5702220.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8213..9022)
                     /gene="agaR"
                     /locus_tag="ACHKAY_003610"
     CDS             complement(8213..9022)
                     /gene="agaR"
                     /locus_tag="ACHKAY_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO5702221.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9271..10551
                     /gene="kbaZ"
                     /locus_tag="ACHKAY_003611"
     CDS             9271..10551
                     /gene="kbaZ"
                     /locus_tag="ACHKAY_003611"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO5702222.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10574..11047
                     /gene="agaV"
                     /locus_tag="ACHKAY_003612"
     CDS             10574..11047
                     /gene="agaV"
                     /locus_tag="ACHKAY_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO5702223.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11058..11849
                     /gene="agaW"
                     /locus_tag="ACHKAY_003613"
     CDS             11058..11849
                     /gene="agaW"
                     /locus_tag="ACHKAY_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO5702224.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAF
                     LPIYFGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFV
                     AAAWLKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11839..12717
                     /gene="agaE"
                     /locus_tag="ACHKAY_003614"
     CDS             11839..12717
                     /gene="agaE"
                     /locus_tag="ACHKAY_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO5702225.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12735..13169
                     /gene="agaF"
                     /locus_tag="ACHKAY_003615"
     CDS             12735..13169
                     /gene="agaF"
                     /locus_tag="ACHKAY_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO5702226.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13166..14299
                     /gene="nagA"
                     /locus_tag="ACHKAY_003616"
     CDS             13166..14299
                     /gene="nagA"
                     /locus_tag="ACHKAY_003616"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5702227.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14650..15804
                     /gene="agaS"
                     /locus_tag="ACHKAY_003617"
     CDS             14650..15804
                     /gene="agaS"
                     /locus_tag="ACHKAY_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO5702228.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGM
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15817..16677
                     /gene="kbaY"
                     /locus_tag="ACHKAY_003618"
     CDS             15817..16677
                     /gene="kbaY"
                     /locus_tag="ACHKAY_003618"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO5702229.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         4062 a         4454 c         4276 g         3984 t
ORIGIN      
        1 agaagactct agctcagaag agttactttc agataacacc ctcgagaaag acgaaaattc
       61 tccggacaat ggttttgagg tcggggaata taacacacat gaagcatata actcagagaa
      121 tcaatatttt accagagagg actatacgta taattacgac cttttaaatg caatatagac
      181 ttaataactt cttacaaagt ctattattaa taccgtagat atttattcat cttaccctgt
      241 gcaccaaaag caaaaactca ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc
      301 tgatgcgcta cgcttatcag gcctacgcca tctctgcaat atattgaatt tgcgtgcttt
      361 tgtaggcagg ataaggcgtt cacgccgcat ccggcatgaa taaagcgcac tcaacaatct
      421 gaaacccgcc ggaacggttt attacgtaca tcaggtgaaa ctgaccgata agccgctttc
      481 ttttgggtat agcgtcgtgg acagtcattc atctttctgc ccctccaaaa gcaaaaaccc
      541 gccggtttac gctggcgggt tttcgttaaa tcaggtgaaa ctgaccgata agccgggttc
      601 tgtcgtggac agtcattcat ctaggccagc aatcgctcac tggctcaagc agcctacccg
      661 ggttcagtac gggccgtacc ttatgaaccc ctatttggcc ttgctccggg tggagtttac
      721 cgtgccacgg actgttacca gccgcgcggt gcgctcttac cgcacccttt cacccttacc
      781 tgatcccgct tgcgcgggcc atcggcggtt tgctctctgt tgcactggtc gtgggtttcc
      841 cccccaggcg ttacctggca ccctgcccta tggagcccgg actttcctcc cctccgcccg
      901 tctcccccga agaggacgac gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta
      961 tagagggttt gcgcgccctt gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc
     1021 gatattacgt gaagcacggc agatattgtc ataagccccg cggaatgctt cgtccaacgt
     1081 accaatgctg gtcagtacgc tgaagacagc atcaatgcca tgctgatgta caacgccaac
     1141 atcatcggtc aggctacccg caatgccaat caccggttta tggtacttct tcgccacgtt
     1201 tgcgacgcca atcggtacct tgccgtgaat actctggctg tcaatacgcc cttcaccggt
     1261 gatcaccagc gtacaatcgt gaatatgctc ctccagattc agcgccgtag tgacgatttc
     1321 aataccactt ttcagttccg caccaagaaa cgccattagc gccgcgccca taccacccgc
     1381 aactcctgcg ccggggacat ctttcacatc aacatgcagc gcttttttaa tgacctcggc
     1441 atagtgagag aggttattgt ccagctcaac aatcatcgct tcactggctc ccttttgtgg
     1501 gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat cacaagcgac
     1561 gcgaatgacg caatctttta agcgcggatc gaggccggaa atatcaatat cattcagagt
     1621 attaagacta ccgccgccaa aaccaatttc attgccgttg gcgtcgcata atttcgcccc
     1681 cagcgcctgt accatgcctg cgccgccatc atttgtagcg ctaccgccaa tgccgataat
     1741 aatgtttgtc gcgccgctct ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt
     1801 ggtcacgagt ggatcgcgtt tttccgcagg taccagctcc agcccactgg ccgccgccat
     1861 ttcaataaac gcggttttgc catcgccgga gatcccccaa ctggcattca ctttctcgcc
     1921 cagcggccct gtaacccagg cgtgacgttc agaaccctgg gtggctgcaa tcatcgcttc
     1981 caccgttcct tcgccaccgt cggcaaccgg aatagaaacg tactgtgcat caggaaaaat
     2041 ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg gcagataaac tttctttata
     2101 agagtctggg gcgattacga ttttcatacc tatgcctgtt accacatgac gccggagggc
     2161 gtttctctta ttcggcctgg attccaggcc cggattgcaa tacgccatcc gggcacgacg
     2221 tcattaacga gtaacttcga ctttcgccag tttttcgtag tagcacgcca gggcgctatg
     2281 atccgccgtt cctaaaccat ctgctcgcag tgcctgcatc atctccataa ccgcagctgt
     2341 gagcggcagt tgcgcgccga cgccgtggga agtatccagc gcatttgcca gatccttaat
     2401 atgcagatca atacggaagc ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc
     2461 atccagcacg gtactgcccg ccagtccacc gcgaattgcc tgatacacca ggtccgggtt
     2521 aacgcccgct ttggttgcca gcgttagcgc ttctgacatc gcggcaatat tcagcgccac
     2581 aatgacctga tttgccagtt tggtgacgtt acctgcaccg atttccccgg tatgcaccac
     2641 ggaacccgcc atcgctttca tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc
     2701 caccatcact gacagcgtac cgtcgatggc tttcggttca ccgccgctca ccggagcatc
     2761 cagcatatca atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc
     2821 gatagaactc atatcgatca ataccgtacc tggcttcgcg ccttcaataa tgccattctc
     2881 acccagcgcc acctctttca catgagggga gtttggcagc atggttatga tgacgtcgca
     2941 ctgttcagcg atcgctttag ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc
     3001 aatagcttct gggttacggt cagcaaccac cagcgagtaa cctgctttca gaaggttttt
     3061 actcattggt ttacccataa tccccaggcc aataaaacca actttcatag tcatatcaat
     3121 catctctctt gttgcggtgg tggttatttt ttaaaggtat cagccagttt ctgagtggca
     3181 gagcggaaga cgccaagatc gctgccgaca gccacaaacg tcgcgcccca ttccagataa
     3241 cgacgcgcat cggcttcgac cggcgcgaga ataccgctgg gtttgccgtg cgcactggca
     3301 cggttaaaaa tgtgctgaat tgctttttgt acatccgggt gcgaggcatt gccgagatgg
     3361 cctaatgccg cagccagatc gctggggccg acgaagatac cgtctacacc ttcggtagcg
     3421 gcaatggcat cgacgttatc tacgccctgc tgactttcta tctggaccag aatggtgatg
     3481 ttcttgttcg actgagcgaa ataatccgcc acggtgccaa acatattggc gcggtgagaa
     3541 acggagacgc cgcgaatgcc ttccggtggg taacgggttg atgccaccgc ctgctctgct
     3601 tcctcttttg tttctacaaa aggaatcagg aagttataga aaccgatatc cagaagacgc
     3661 ttaataatta ccggctcgtt ggtcggcact cgcactactg gcgcgctggc gctgcctttc
     3721 agggccatta actgcggaat aaacgtggag atatcgtttg gcgcatgttc gccatccagc
     3781 accagccagt caaacccagc caaaccaaga acttcagtgc taatcgggtt agagagtgct
     3841 gaccagcaac caatttgtac ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa
     3901 acatcgttat tcatcgctta tacctttgct tatttctgca attccatacg tttaatgtcg
     3961 ccaactacga agaggtagca gaccatcgcc atcagcgctg aacatcccac gaaaaccagt
     4021 gctgcattga aggagtgcag ttcacttacc aggtagccaa tcaccagtgg agtgacaatg
     4081 gaggcaacat tgccaaagac gttaaagacg ccgccacaga ggccaacaat ctctttcggc
     4141 gcggtgtcag aaatcaccgg ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc
     4201 gccatcagca tgaccaccag cgtggtgttg ttggtgtagt tacataagat gatggtggaa
     4261 gccagcaaca ttcccagcac aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt
     4321 ttgatcagat aatccgagaa gacacctccc agcacgccgc ccgcaaaacc acacagtgcc
     4381 ggaatcgagg cgaccagacc cactttcaga atcgacatgc ctttttcctg caccagataa
     4441 atcgggaacc aggtgaggaa gaaccaggtg atggtgttga taaaatattg tccgaaaaat
     4501 acgcccaaca tcatgcggtt agagagcaat tgcttgatgt aatgcagttt gggaccgctt
     4561 gctgccgcgc tgcccggctt tttgtggtcc atatcgacca ccgcgccatt ttcagagata
     4621 aacttcagct cttccgcaga catacgtggg tgatctgtcg ggttatgaat caatttgatc
     4681 cacagcgccg tcagcacaaa accaatcacc cccataacgg taaagacgtg ctcccagccc
     4741 caggcgaaag tcagccagcc aagcagaggc gaaaagagcg ccagcgagaa atattgcgcc
     4801 gagttaaaga tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata
     4861 cgggcgttcg ccgggaatga tggcgcttcc gagaagccga gcataaagcg cataaagaac
     4921 atggagatcc ctgcccaggc cagcgggaac atatcaacaa agccttgcag gaaagtgaat
     4981 agcgaccaga aaaagaggct ataggtataa acttttttcg agccaaactt atcaagcagc
     5041 cagccgccgg ggatttgcat cagcaagtag gcccagccaa aagcagaaaa gatgtaaccc
     5101 atcgaaaccg cacttaactg caactctttt gccacttcgg taccagcaat agacagcgtt
     5161 gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa tcagtaatat taaatagcgg
     5221 gtatgcacgc ctttcttttt ttcgtcaacg gtgtccagaa tcattttatt tacctcgggt
     5281 acttatgctg atttttatta ttatggggaa ggtgttattt atgagtttca tttatgccgt
     5341 aacggcaatg aactcgggaa ttagtataag cagcgcgaga ataataatca ttgtgcaaat
     5401 gctaatttaa ttaatgctat ttaaatatta ttttgagcat atgcacataa ggttgcgcgc
     5461 taaagcacag atttgcgctt taccttgccg ggcggcactg caatccctga aatgattgac
     5521 attgatcaca tttctgcgtt taaactcctg acattcttat ttcacccaat gaagtcattt
     5581 atttttaaat gagaccaggt cctcatttta ataacccctg gctggagaat atagtacaat
     5641 ggccaacatc gaaatcagac aagaaacgcc aactgcgttt tatataaaag ttcacgacac
     5701 agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc gttttccgga
     5761 tggactggaa ttaattgaac atattcccca ggggcataaa gtcgcattgc tggacattcc
     5821 ggctaatggt gaaattattc gttatggtga agtgattggt tacgccgtgc gtgcaatccc
     5881 acgcggaagc tggctcgacg aatcaatggt tgtactaccg gaagcgccgc cgttacacac
     5941 gctgccactg gcaaccaaag tcccggcccc cttaccgccg ctggaaggat acacctttga
     6001 gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca tcaccaccag
     6061 cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa atcattgaac gcgatctgct
     6121 accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg gctgtggcgt
     6181 ggcgattaac gcaccggcgg cagttgtgcc tattcgtacc atccacaata tttcgctgaa
     6241 ccctaacttt ggcggcgaag taatggtgat tggtctgggt tgtgaaaagt tgcagcctga
     6301 gcgcctgctg gtcggcacgg atgatgtgca agctattcca gtagaaagcg ccagcattgt
     6361 cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata ttttgcaggt
     6421 cgccgaacgc catctacaaa aactgaatca acgtcagcga gaaacctgtc cggcttcaga
     6481 actggttgtc ggcatgcagt gcggtggcag cgatgccttt tctggcgtaa cggcaaaccc
     6541 ggcggttggc tatgcgtctg atctactggt gcgctgcggc gcaacggtga tgttctcaga
     6601 agtcacggaa gtgcgtgacg cgatccatct gctgacacca cgcgcagtga acgaagaggt
     6661 cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata tgggaaaaac
     6721 cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc ggtctggcaa acgtggtaga
     6781 gaaggcactc ggctccattg ctaaatcggg taaaagcgca attgttgaag tgctgtcgcc
     6841 cggtcaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt
     6901 ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg
     6961 tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcgacccgca ccgagctggc
     7021 gaaccgctgg tttgatttaa tggatattaa cgcgggcact atcgccaccg gcgaagaaac
     7081 cattgaagag gtgggctgga agttgttcca ctttattctc gacatcgcca gcgggaagaa
     7141 gaaaaccttc tcggatcaat gggggctgca taaccagctg gcagtgttta acccggcacc
     7201 ggtgacctga tttcttttgg aacgtccctc gcaaaacatg gccttagtgc catgttttta
     7261 ttgtttaaag cccccacgtc cattaataat gcatttgcat taccttagtt caagcttata
     7321 atttgagcag aaaacaggct gtaaaaggac agtgaatcat gcccgctaat gctcgctctc
     7381 acgctgtact gaccactgaa tcaaaggtca cgatacgcgg acaaacaact atccccgcac
     7441 cagtgcgtga ggccttaaaa ctgaagccag gccaggacag cattcattac gaaattctgc
     7501 ctggtgggca agtctttatg tgccgactgg gagatgaaca ggaggatcat actatgaatg
     7561 catttttgcg ttttctggat gcagatatcc agaacaaccc gcaaaaaact cgtccattca
     7621 acattcaaca aggaaagaaa cttgtcgctg gcatggacgt caacattgat gatgagattg
     7681 gcgacgacga ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc
     7741 ctgttttcag gaaacctacg acgctttagt tgccgaagtc gagacattaa agggaaaaga
     7801 tcctgaaaat tatcagagaa aagccgccac aaagttattg gcggtagtcc ataaagtgat
     7861 tgaggagcat atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg
     7921 ctctgggaaa aataaagact ggtcacgggt aaaatttggt gctggtcgtt atcgtctctt
     7981 ctttcgttat agtgaaaaag agaaagtcat cattctggga tggatgaacg atgaaaacac
     8041 tctgcgcacc tacggtaaaa aaacagatgc ctataccgta ttcagcaaaa tgttaaaaag
     8101 aggacatcct cctgccgact gggaaaccct cacccgagaa acagaagaaa cccattgatg
     8161 gtgttcacaa tgcccgccga acgtattcgt gttgaccagc gggcaccctt tctcactccc
     8221 cgaccagaat cacttcaacc ccagcctttc gcagtccttc cagactatcc gcaggaatgc
     8281 cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactggaac
     8341 gattgaattt actggaatcg gtgaccacga tgatccgttc cacaacttcg cacatccgac
     8401 ggtttaaacg ggcttcatct tcattatgtg tgctgacgcc gcgctccaga tcgatcgcat
     8461 ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcgc
     8521 cgtaaaaaga ttgcgactga cggcgcaaat gcccgccggt catcagcagc tcaacgcctt
     8581 ccgcttccag caacgcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt
     8641 gcttacgcat cagacgagca atctcaaaag tggtagtccc ggaatcgagg atcacccgat
     8701 gaccaggctg aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca
     8761 gcgcgctttt atcttccact gatggctcga ctgacggcgt cgtgctatcg cagatcaacg
     8821 cgccaccata ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcattgcgga
     8881 tcgtcacagt agatacgcca tacaatgccg acagatcgtt aacctgcaca ctcccttgct
     8941 gtcgcagacg ctgaatgatc tgttctcgtc gctcgctggt gccagtcact cgcttctcac
     9001 ctgaagcgtc ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt
     9061 tgcctattaa cgcctttcta ttaagcaaat gcaagcccac cttgcccatt gacgcaagct
     9121 actctcgttt cagtgacttt cattatgttt cttttgtgaa tcagatcaga aaactattat
     9181 ctttcgtttt atttttatct caccatgacg cagtatcaac tgaaacaaaa cgaaagatta
     9241 atatcgcagt aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag
     9301 cacaaagcgg gcaaaacaaa tggaatttat gccgtttgtt ccgcacatcc gctggtgctg
     9361 gaggctgcaa tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc
     9421 aatcaggtag accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt
     9481 gtttgtcagc tcgccgactc gttgaatttc ccgcaggatg cgttgattct gggtggtgac
     9541 catctggggc caaaccgctg gcaaaacctg ccggccgctc aggcaatggc caatgccgat
     9601 gatttgatta aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg
     9661 tcctgtcatg acgatccgat tcccttaact gatgacatcg tggctgaacg cgccgcccgt
     9721 ctggcgaaag tggcggaaga aacctgtctt gaacactttg gcgaagccga tctggagtat
     9781 gtcattggta ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg
     9841 gtcaccacgc cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ctttgaaaag
     9901 caaggtttga atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa
     9961 ttcgatcaca ccaacgttat tgattatcag cccgccaaag cgagcgcctt aagccagatg
    10021 gtcgaaaact acgaaacgct gattttcgaa gcgcactcta ccgattatca aacgccgcaa
    10081 tcgctgcgcc agctggtgat tgaccacttt gccattctga aagttggccc agcgctgacc
    10141 ttcgccctgc gtgaagctct gttctctctg gcggcgattg aagaagaact ggtgccagcg
    10201 aaagcctgtt ctggtctgcg tcaggtgctg gaagacgtga tgctcgaccg cccggaatac
    10261 tggcaaagcc actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac
    10321 tcggatcgcg tgcgctatta ctggccggac agccagattg atgacgcttt cgctcatctg
    10381 gttcgtaatc tggcggattc accaattccg ctaccgctca tcagccagta tctgccgctg
    10441 caatacgtga aagttcgctc cggcgagctg caaccgacgc cacgggaact cattatcaac
    10501 catattcagg acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa
    10561 agaggaacac gctatgccaa atattgtttt aagccggatt gatgaacgct tgattcacgg
    10621 tcaggtcggc gttcagtggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga
    10681 tgaggttgcc gaagatccgg tacaacaaaa cctgatggaa atggtactgg cagaagggat
    10741 cgccgtacgt ttctggacgc tgcaaaaagt tatcgacaac attcatcgcg ccgccgatcg
    10801 acagaaaatc ctgctggttt gtaaaacacc cgccgatttc ctgacgctgg tgaaaggtgg
    10861 cgttccggtg aatcgcatta acgttggcaa tatgcactac gccaatggca aacaacaaat
    10921 cgccaaaacg gtttctgtgg atgcgggcga tatcgcagca tttaacgacc tgaaagccgc
    10981 tggggtggaa tgcttcgttc agggcgtccc gacagagcct gctgtagacc tctttaaatt
    11041 actttgaggg attcatcatg gaaatcagcc tgttgcaggc atttgcgttg ggcattatcg
    11101 cctttatcgc tggcctggat atgtttaacg gcctaacaca tatgcaccgc ccggtggtcc
    11161 tcggcccgtt ggtcgggctg gtacttggcg atctgcatac cggaatttta accggcggta
    11221 cgctggaact ggtgtggatg gggctggccc cgctggcggg cgcacagccg cctaacgtga
    11281 ttatcggtac tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg
    11341 cggtcggtgt cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct
    11401 cggtgatgtc tggcgtgatg tctggcgtga tgtctcgctg cgaccggatg gcggagaacg
    11461 ccgatactcg cggcattgaa cgcgtgaact atctggcact gctggcactc ggcactttct
    11521 attttctctg cgctttcctg cctatctact tcggcgcgga acatgcaaaa accatcattg
    11581 atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa
    11641 tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt ctacatcccc tacttcatcc
    11701 tgggctttgt tgccgcagcc tggctcaagt taccggtgct ggctattgct gccgctgcgc
    11761 tggcgatggc gctgatcgac ctgctgcgta aatctcctga accgactcaa cctgcggcac
    11821 agaaagagga atttgaagat ggcatctaat caaaccaccc tgccgaacgt cagtgaaaac
    11881 gaagaaacat tgctgactgg cgtcaatgaa aacgtgtatg aagatcagag cattggcgcg
    11941 gagctgacga aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt acaagcgtct
    12001 tttaactacg aacgtatgca ggcttctggc tggttgtacg gtctgctgcc tgctctgaaa
    12061 aagatccaca ctaataaacg cgacctggcg cgcgccatga aggggcatat gggtttcttc
    12121 aatacccatc cgtttctggt gacatttgtt atcggcatta tccttgcgat ggagcgttct
    12181 aagcaggacg ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc gctcggcggg
    12241 attggcgatg caatgttctg gctaacgcta ctgccgattt gtggcgggat tggagccagt
    12301 ctggcattgc aaggctccat tcttggcgca gtcgtcttta ttctgctgtt caacgtggta
    12361 cacctggggc tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt
    12421 ccactcatta aagcgaatac caaaaaagtc ggccatgctg cgtctatcgt tgggatgacg
    12481 gtaatcggtg cgctggtggc aacctatgtt cgtttaagca ccacgctgga aatcacagcg
    12541 ggcgacgcag tggttaagtt acaggctgat gttatcgaca aactgatgcc tgccttctta
    12601 ccgttggtct acaccctgac catgttttgg ttggtacgcc gcggctggag tccgctgcgt
    12661 ctgattgcag ttaccgtggt tctcggcatc gtcggtaaat tctgccattt cctttaaata
    12721 caatgaggta ttagatgtta agtattattt tgacagggca tggcgggttt gccagcggca
    12781 tggaaaaagc aatgaagcaa attctcggtg agcaatcgca gtttatcgcc atcgattttc
    12841 cggaaacctc aagcaccgcg ctgcttacat cgcagcttga agaggccatc gcccaactgg
    12901 attgtgaaga cggcattgtt tttctgacgg atttgctggg cggcacgccg tttcgcgtag
    12961 cttcaacgct ggcgctgcaa aaaccgggct gtgaagtgat caccggcacc aatttgcaac
    13021 tgctactgga gatggtgctg gagcgcgaag ggttaagcgg tgaagaattc cgtgtacagg
    13081 cgctggagtg tggacatcgt gggctgacca gtctggtgga cgagttaggc cgctgccatg
    13141 aagaatgtcc ggtcgaggaa gggatatgac acacgttctg cgcgccagaa ggctgctgac
    13201 tgaagaggga tggctcgatg accatcagtt gcgtattgct gacggtgtca tcgcagcaat
    13261 cgaaccgatt ccagtgggcg tgactgaacg cgatgcggaa ctgctctgcc ccgcttacat
    13321 cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact
    13381 cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc agttggttgc cgactaccgt
    13441 aaccgcgccg cttaacacca ttcatgcggc gctgaaacgt attgctcaac gttgccaacg
    13501 cggcggacct ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact tcacgccgca
    13561 gaataaaggc gcgcatccgc cggaattgtt tcgcgagctt gaaattgccg agctggatca
    13621 attgattgcc gtttctcagc acaccttacg cgtggtagcg ctggcaccgg aaaaagaggg
    13681 ggcattgcag gccatccgcc atcttaaaca gcaaaatgta cgagtgatgc tggggcatag
    13741 cgcggcgacc tggcaacaaa ctcgcgccgc gtttgatgct ggtgccgacg gcctggtgca
    13801 ttgctataac gggatgacag gtttacatca ccgcgaaccg ggaatggttg gcgcgggatt
    13861 aacggacaag cgcgcctggc tggaactgat agccgatggt catcatgtgc atccggcggc
    13921 gatgtcgctg tgttgttgct gtgcaaaaga gagaatcgtg ctgatcaccg acgcgatgca
    13981 ggcagccggg atgccggatg gtcgctatac gttatgtggc gaagaagtgc agatgcacgg
    14041 tggcgttgtc cgtaccgcgt ccggtgggct ggcgggcagt acgctgtctg ttgatgcggc
    14101 agtgcgcaac atggtcgagt tgacgggcgt aacgcctgcg gaagccattc atatggcatc
    14161 gctgcatccg gcgcgaatgc tgggtgttga tggtgttctg ggatcgctta aaccgggcaa
    14221 acgcgccagc atcgttgcgc tggatagcgg gctgcatgtg caacaaatct ggattcagag
    14281 tcaattagct tcgttttgat attttgctcc tttattgggc cttcacttcc cccgtaaggc
    14341 ctttcttttt ctttcgtttt gatctgtgca gcggtgtcgg atgcgacgct aacgcgtctt
    14401 atgcgaccta cggtacgccg catccagcaa tcggtgaaca tagcgacgtc attacgcaat
    14461 tctttaatct ccccttgtct tttctttcaa tgatcacaga tttcgtttta tttcctttct
    14521 ctccattgaa ctttcggttt cttttctata gattttaatc aacgaaagac atcaccaagt
    14581 gaaatgaaac gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca tcagactaag
    14641 gattgagtta tgccagaaaa ttacacccct gctgccgccg caaccggtac atggactgaa
    14701 gaagagatcc gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta
    14761 cgttctgcgc tcaataactt ccttgaaccg ttactgcgca aagagaatct gcggatcatc
    14821 ctgaccggag ccggaacctc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc
    14881 cataccggta aaaacttcag cgccgtaccg accaccgatc tggtcaccaa tccgatggac
    14941 tacctgaacc cagctcatcc gctgctgttg atctccttcg gtcgatccgg caacagcccg
    15001 gaaagcgtcg ctgccgtgga actggcaaat caatttgtac cagaatgcta tcacctgccg
    15061 atcacctgca acgaagcggg cgctctttac caaaacgcga tcaacagcga taacgcgttt
    15121 gccctgctga tgcccgcaga aacgcacgat cgcggctttg cgatgaccag cagcattacc
    15181 accatgatgg ccagctgcct cgcggttttc gcacctgaga cgatcaacag ccaaaccttc
    15241 cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt
    15301 atgtttggtt acgcaccgtg gaaacggatc gtttatctcg gcagcggtgg cttacagggc
    15361 gcagcacgcg agtcggcgct gaaagtgctg gaactgactg cgggtaaact ggcggccttc
    15421 tatgattccc cgaccggatt ccgtcatggc ccgaaatcac tggtcgataa cgaaacgctg
    15481 gtggtggtat ttgtctcaag ccacccttac acccgtcagt atgatcttga tctgctggct
    15541 gaactccgcc gtgacaacca ggcaatgcgc gtaatcgcca tcgccgcgga aagcaccgac
    15601 atcgtcgctg ccggtccaca tattatcctg ccgccgtcac gtcactttat cgacgttgag
    15661 caggcatttt gcttcctgat gtacgcccag acgtttgcac tgatgcagtc gctgcacatg
    15721 ggcaatacgc cggatacccc atcagccagc ggcaccgtta accgcgtggt gcaaggcgta
    15781 atcattcatc cgtggcaggc ataagaggat cgcattatga gcattatctc cactaaatat
    15841 ctgttacagg acgcccaggc caatggctac gcggtgcctg cttttaacat tcataacgcc
    15901 gagacgatcc aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc
    15961 ggaacgccgg ggacctttaa acatatcgcg ctggaagaga tctacgccct gtgcagcgcc
    16021 tattccacaa cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat
    16081 gatattcgcc gtaaagtcca cgcaggtgtg cgcagtgcaa tgatcgacgg cagccacttc
    16141 ccgtttgccg agaacgtgaa gctggtgaaa tcggttgttg acttctgcca ctcacaggat
    16201 tgcagcgtgg aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac
    16261 gccgaaagtg cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc
    16321 gtcgacagcc tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag
    16381 attgatttcc agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctacat
    16441 ggtgccagcg atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa
    16501 gtgaacgttg ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg
    16561 gaaaatccgc agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa
    16621 gaagttgtca gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca
    16681 ttagtttttt atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg
    16741 gaaaaggaga tgactaaacc ttaataaata catcag
//
LOCUS       DAZFKM010000106        16615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFKM010000106 DAZFKM010000000
VERSION     DAZFKM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..189)
                     /locus_tag="ACHKAY_003619"
     CDS             complement(37..189)
                     /locus_tag="ACHKAY_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5702230.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHKAY_003620"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHKAY_003620"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO5702231.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHKAY_003621"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHKAY_003621"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO5702232.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHKAY_003622"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHKAY_003622"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO5702233.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHKAY_003623"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHKAY_003623"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO5702234.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHKAY_003624"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHKAY_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO5702235.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHKAY_003625"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHKAY_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO5702236.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHKAY_003626"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHKAY_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO5702237.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHKAY_003627"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHKAY_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO5702238.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHKAY_003628"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHKAY_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO5702239.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHKAY_003629"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHKAY_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5702240.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DYIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHKAY_003630"
     CDS             complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHKAY_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO5702241.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHKAY_003631"
                     /pseudo
     CDS             complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHKAY_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13466..14743
                     /gene="yqcE"
                     /locus_tag="ACHKAY_003632"
     CDS             13466..14743
                     /gene="yqcE"
                     /locus_tag="ACHKAY_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO5702242.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14770..16248
                     /gene="ygcE"
                     /locus_tag="ACHKAY_003633"
     CDS             14770..16248
                     /gene="ygcE"
                     /locus_tag="ACHKAY_003633"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO5702243.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3955 a         4362 c         4180 g         4118 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcacctac
      121 catcagcgtg aatcccagta ctgtacaaca cagtacgatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatcg cccaccgata aatatccttc gtcccataat gggtgatatt tcaggccatg
      601 tttttgcaga tactgatata tcgttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
      841 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggcgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctctgcttcc gccatcgcca gcgggcaagt cgggaacgag acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaccc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcg tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttgtcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctga tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 cgggttagcg ggcaggcgaa agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cgatccgcga ggaagctgga ggcaccaccg gcacgggctc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc
     3661 cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
     4261 cttgctgtcg aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ctacgatgag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac taaaagcggc gtttaaacca
     5281 acctacgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc
     5341 agcgaggttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgtaccgca ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctcccgca atttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa gaagaaggtg
     5881 tcgaatgcat ccccggagcg acggtggatg cactgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgtg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgagcgt cacgggttag tgactcgtcc tgctggtgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaac catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcaggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagtcgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttgc gctttatcat cacaacgtag agcgcagcct gctatgggat gttctacaac
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga
     6961 gtgtggcacc tgctgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggcaatcga ctctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtga ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc accgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcac gggtaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgcg
     8221 ccggggacaa tgttcagttg ctgcatacca gagggcaacg tggcgttttt cactgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatagct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caaaccatgt tcaatacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatagt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac acattttccg
    11461 tctgcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgtgcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acttcctggg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggaacc gtcgagcacc
    12001 accgagtttt ctacaacagt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 gattagcgca ctaaataacc accatcgacc actaataaat gcccattgac ataatttgaa
    12421 gccggacttg cgaggaatac agctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcaata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaatccggca agagcatgtt tagtggcaga atatgcaggt
    12661 gaccattgtc cacctaagta agagaacaat gaacagatat taataatttt gccgcttttc
    12721 tgcgggatca taatttttgc agcttcatag cttaactcga atgcggcggt caggttcaca
    12781 tcaatcatcg gatcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata
    12841 ccggcattgt ttaccagaat atcaactgta ccgaaacgct cacagcaagc agcgataatc
    12901 ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctgt
    12961 ttttcaatca tttcctttgt ttcgccgtta tctttgacga aactaggaat aaagatattt
    13021 gcgccagctt tggccaacgc catggcaaat gcctagccta aaccgctatt cccaccggta
    13081 acaattgcgg ttttaccttt cagtgagaaa aaatccattg agaacgcatt gagagattcg
    13141 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa gaaaggtaac ttcccccgcg
    13201 agagggaagt tacctttctc attatctctg gtaactggag ataattttta gcataacggc
    13261 ataactgcta atgtgatcgt aatcacagtg tgatattcgt attgtcaaat agttttaaaa
    13321 agcagaagcg tgattactct cacacaacac ctctgctttc catgttattt cttattgcaa
    13381 ttactagaga cctgagaaaa gtaatccacc tgaaagggcg gcttactttt ctcttttttt
    13441 tgtctattac aaatgggaat acgatatgca acacaactca tatcgccgtt ggataaccct
    13501 cgcgataatt agttttagcg gcggcgttag ttttgacctg gcttatttac gttatattta
    13561 tcaaattccc atggcgaaat ttatgggatt cagcaatacc gagataggtt taataatgag
    13621 tacctttggt attgcggcta ttattcttta tgcccccagc ggcgttattg ccgataaatt
    13681 ttcacaccgc aaaatgatta cttccgcgat gatcattacc ggattactgg gtctgttaat
    13741 ggcaacgtat ccaccgctgt gggtaatgct ctgtattcag gtcgcctttg cgataacgac
    13801 gattttaatg ctgtggtcgg tgtcgattaa agccgcctcg ttgctgggcg atcatagtga
    13861 gcaagggaaa attatgggct ggatggaagg gctgcgcggc gtcggtgtaa tgtcgctggc
    13921 ggtgtttacc atgtgggtct tttctcgctt tgcaccggat gacagcgcca gcctgaaaac
    13981 agtcattatc atctacagtg tggtttacat cttgttgggg attctgtgct ggttttttgt
    14041 tagcgataac aacaacctgc gcagtgccaa taacgaagaa aaacagtcat tccagcttag
    14101 cgacatcctt gccgttttgc gtatcagcac cacctggtat tgcagcatgg tgatttttgg
    14161 tgtcttcaca atctacgcca ttctgagtta ctccaccaac tatctgaccg aaatgtacgg
    14221 catgtcgctg gtggcagcga gctacatggg gattgtgatc aacaaaatct tccgcgcgct
    14281 gtgcggccca cttggcggca ttatcaccac ctacagtaaa gtgaaatccc ctacccgcgt
    14341 gatccaaatc ctttccgtac tcggcctgct ggcgttaact gccctgctcg tcacgaactc
    14401 taacccgcaa tcggtcgcga tggggattgg cctgatttta ctgctgggat tcacctgtta
    14461 cgcctcacgc gggctgtact gggcctgccc tggcgaagtg agaacaccgt cttacattat
    14521 gggcaccacg gtaggtattt gttcggtgat tggattcctg ccggatgtct tcgtttaccc
    14581 gattatcggc cactggcaag acactctgcc cgcagcagaa gcctaccgca atatgtggct
    14641 gatgggaatg gcggcgcttg ccatggtgat tgtctttacc tttttgctgt tccaaaaaat
    14701 tcgtactgct gatagcgccc ccgcaatggc tagcagcaag taagccagga aaattacagg
    14761 agaaaaatca tgtcgaaaaa atacatcata gggattgatg gcggaagtca gagcacaaaa
    14821 gtggtgatgt acgatctgga aggtagtgtg gtttgcgaag gtaaaggctt attacagccg
    14881 atgcacacgc cttatgccga tactgcagaa catcctgacg acgatttatg ggcatcatta
    14941 tgttttgcag gtcacgattt gatgagccag tttgccggga ataaagaaga tattgttggc
    15001 atcggcctgg gctccatccg ttgctgccgt gcgttattga aagccgatgg cacgcctgct
    15061 gcgccgttga ttagctggca ggatgcacgc gttacacgcc cttacgaaca tacgaatcct
    15121 gacgtggcgt atgtcacctc tttttcgggt tatctgacgc atcgcttaac cggcgagttt
    15181 aaagacaata tcgccaacta ttttggtcag tggccggtgg attataagag ctgggcatgg
    15241 agcgaagatg ctgcggtaat ggataagttt aatatccccc gtcatatgct gtttgatgtg
    15301 caaatgcctg gcaccgtcct cggacatatc acaccacaag ccgcactggc gacacatttc
    15361 ccggcaggac tgccggttgt ttgtaccacc agtgataaac cggtagaagc tctgggggcc
    15421 ggattactgg atgatgaaac tgcggtaatt tctcttggca cttacatcgc attgatgatg
    15481 aacggcaaag cactgccgaa agatccggtg gcgtactggc cgattatgtc ttctattccg
    15541 caaacattgc tgtatgaagg ttacggtatt cgcaaaggta tgtggacggt gagctggctg
    15601 cgtgacatgt taggcgagtc gttaattcag gatgccaggg cgcaggatct ttcaccggaa
    15661 gatttactca acaagaaagc ctctagtgtg ccacctggct gtaatgggct gatgacggtg
    15721 ctggactggc tgaccaatcc gtgggaaccg tacaaacgcg ggattatgat cggctttgat
    15781 tccagcatgg attacgcatg gatatatcgt tcgatattgg aaagcgtggc gctaacgctg
    15841 aagaacaatt acgacaatat gtgtaatgaa atgaatcact ttgcgaagca tgtgatcatt
    15901 actggcggcg gttcgaacag cgatctgttt atgcaaattt ttgccgacgt gttcaacctt
    15961 ccggcacgcc gtaacgccat taacggttgt gcaagtctgg gagcagcgat taatacagcg
    16021 gtaggtctgg ggctataccc ggattacgca acggctgttg ataagatggt tcgcgtgaaa
    16081 gatatcttta taccgattga gagcaatgcc aaacgctacg acgcgatgaa taaaggcatt
    16141 ttcaaagacc taaccaaaca tactgatgtg atcctgaaaa aatcgtatga agtgatgcat
    16201 ggggaattgg ggaatgtgga ttcgatccag agctggtcga atgcgtgaat gaggtaggcc
    16261 gggcggaaaa ctgcccggcc tgaacatacc taaatggtta tccccgctga cgcggggaac
    16321 ataagttcga caacttaaaa aatgataaca agtccggttt atccccgctg atgcagggaa
    16381 cataataaaa actttaacta gttcggttta tccctgtttt tacaaggaat attaactaca
    16441 ctcgatgtct ttagaattaa agacaatacg aatcgcactt tatcaatttt cacttaaagt
    16501 atgaactgga tatctttatt cataagaact attcatcaac agcgtataga ggcggttatg
    16561 taaaaccact cattagcctc aaaacaaaga caaacttcca gatttattac ttaac
//
LOCUS       DAZFKM010000107        16297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFKM010000107 DAZFKM010000000
VERSION     DAZFKM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..606
                     /locus_tag="ACHKAY_003634"
     CDS             <1..606
                     /locus_tag="ACHKAY_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO5702244.1"
                     /translation="MSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAIT
                     RSSGDRHQFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYM
                     EGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAAAFSITLARGRAELYPEMHGTVT
                     GFKSEIDNQDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAE"
     gene            complement(771..1406)
                     /gene="hxsA"
                     /locus_tag="ACHKAY_003635"
     CDS             complement(771..1406)
                     /gene="hxsA"
                     /locus_tag="ACHKAY_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser repeat protein HxsA"
                     /protein_id="HFO5702245.1"
                     /translation="MKKFNFAALLPGFLALNNSVWASDSSTGASDLPGMTLNEHDLVI
                     APLNTEVPFYIAGHRSHSSHRSHSSHRSSSGGGYYGGSTPYYPKTYSSPRSSGSSSSG
                     ASSSSSSSSVRALRSNDNNRTTNTSSVGTSEVNRTSNGLASDAEKRKRLIMRVQFALL
                     DRGFYNGNIDGIMGPSTRLSVKNYRIANGLPIPATETLDTQLLNSLNILAR"
     gene            complement(1403..2515)
                     /gene="hxsC"
                     /locus_tag="ACHKAY_003636"
     CDS             complement(1403..2515)
                     /gene="hxsC"
                     /locus_tag="ACHKAY_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsC"
                     /protein_id="HFO5702246.1"
                     /translation="MSEVIRNDIFHFASTENVPTGFYRLCKQKPVNPLFFLPNLLVVA
                     EGNNDAIPPCFDFSVISTELFESIEDGDIGIINNGNMIRVILSRKANHNTVLVTERCN
                     NLCLFCSQPPKKINDDWLLTQSALAIASFGLNGVVGVSGGEPLLYGDDFLHFIDFIIE
                     NSPDTALHVLTNGRKFADINFTQEMAKRSKKIKITFGIPLYSSRPLVHDHLVGSDGAF
                     NETVKGLINAGNLGINIELRVIPTLANYTELDDIVEFAGRVFSNINQISLMGLEPIGW
                     ARKNWSTIFIDHSSYSEKIISAIDAAYRSGIPLTIFNYPLCYLPERARELAVQSISDW
                     KNYYPKECDECTQKPSCAGYFSSSKGRFHQSPRPIL"
     gene            complement(2508..3896)
                     /gene="hxsB"
                     /locus_tag="ACHKAY_003637"
     CDS             complement(2508..3896)
                     /gene="hxsB"
                     /locus_tag="ACHKAY_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HFO5702247.1"
                     /translation="MELMPFNFDRLPNGQVFISNLAGFHHFIGEQDLIDLSDERISSE
                     QSNVLESKLFITSESSSAITPYALSSAFAKRLMNELAVRPIFMIVPTLRCDHTCKYCQ
                     VSRASVNASGYDLNPELIPDIISAIKKLSTPPYKIEIQGGEPLLRFDLIQSIYDQCAE
                     TLGRTNFEMVVASSLSVLNEDMIEWSRDRNITFSVSLDGEEVVHNSNRILGRGLAYSR
                     TVSGVEAIKHSLGAGRVATVTTVTKELIKRPESIVQAHLSLGLKDMFIRPVSPYGFAQ
                     KASFTFSMDEYFSFYASLIDEILKINNEGIRVVEHSASIHLKRIFNPGFSGYADLKSP
                     SGVVLNSILFNYDGRVYGSDESRMLQKVNPETEFSAGEVKTLSFSDSPYYNAVLSSSF
                     NFALPGCDTCAYQPFCGSDPCQNISVQGEPVGDRSRSTFCQYHKGMFRYLMNCISEGG
                     SKAEMLKGWAYV"
     gene            complement(3896..4168)
                     /gene="hxsD"
                     /locus_tag="ACHKAY_003638"
     CDS             complement(3896..4168)
                     /gene="hxsD"
                     /locus_tag="ACHKAY_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HFO5702248.1"
                     /translation="MSERVFEKELYSEWVIRNSLYWMTPLTRWKLHEDVSHWIISFEN
                     DNPKFLYEFDRLLNDYSLREKLQHKTGALRDSIVHKVLRSVDERLS"
     gene            4421..4681
                     /locus_tag="ACHKAY_003639"
     CDS             4421..4681
                     /locus_tag="ACHKAY_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5702249.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGAHSSEGIPWHELPASHKGNNQMSLPLPQN"
     gene            4872..5012
                     /locus_tag="ACHKAY_003640"
     CDS             4872..5012
                     /locus_tag="ACHKAY_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5702250.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(5318..5617)
                     /gene="ihfA"
                     /locus_tag="ACHKAY_003641"
     CDS             complement(5318..5617)
                     /gene="ihfA"
                     /locus_tag="ACHKAY_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO5702251.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(5622..8009)
                     /gene="pheT"
                     /locus_tag="ACHKAY_003642"
     CDS             complement(5622..8009)
                     /gene="pheT"
                     /locus_tag="ACHKAY_003642"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO5702252.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(8024..9007)
                     /gene="pheS"
                     /locus_tag="ACHKAY_003643"
     CDS             complement(8024..9007)
                     /gene="pheS"
                     /locus_tag="ACHKAY_003643"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO5702253.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(9217..9341)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(9291..9335)
                     /gene="pheM"
                     /locus_tag="ACHKAY_003644"
     CDS             complement(9291..9335)
                     /gene="pheM"
                     /locus_tag="ACHKAY_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO5702254.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(9458..9814)
                     /gene="rplT"
                     /locus_tag="ACHKAY_003645"
     CDS             complement(9458..9814)
                     /gene="rplT"
                     /locus_tag="ACHKAY_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO5702255.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(9867..10064)
                     /gene="rpmI"
                     /locus_tag="ACHKAY_003646"
     CDS             complement(9867..10064)
                     /gene="rpmI"
                     /locus_tag="ACHKAY_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO5702256.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(10161..10703)
                     /gene="infC"
                     /locus_tag="ACHKAY_003647"
     CDS             complement(10161..10703)
                     /gene="infC"
                     /locus_tag="ACHKAY_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO5702257.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(10707..12635)
                     /gene="thrS"
                     /locus_tag="ACHKAY_003648"
     CDS             complement(10707..12635)
                     /gene="thrS"
                     /locus_tag="ACHKAY_003648"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO5702258.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            13159..15058
                     /gene="espL1"
                     /locus_tag="ACHKAY_003649"
                     /pseudo
     CDS             13159..15058
                     /gene="espL1"
                     /locus_tag="ACHKAY_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            15230..15337
                     /gene="yniD"
                     /locus_tag="ACHKAY_003650"
     CDS             15230..15337
                     /gene="yniD"
                     /locus_tag="ACHKAY_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO5702259.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(15390..16148)
                     /gene="ydiY"
                     /locus_tag="ACHKAY_003651"
     CDS             complement(15390..16148)
                     /gene="ydiY"
                     /locus_tag="ACHKAY_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO5702260.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         4434 a         4012 c         3579 g         4272 t
ORIGIN      
        1 atgtccttcc ttacgcgcat ggcggaaatg ctgggcgcaa tcaccacagt aaaaagcggc
       61 aatctgttat tcatcatgcc aggtggtggc gtgaacgcac agggccagcc gttgccctcg
      121 ttcgccatta cacgcagcag cggcgatcgc catcagttcc gtattgctga ccgcgaggcg
      181 tatacggggg tacgcgccta ctggcttgat cttaattacg ggaaaaagaa aaaagtcagc
      241 gtgaaacgcc gtaaaccgcc aaaacccaaa aaggagaaaa gcagcagccg tgaaggtgat
      301 tatatggaag gcgcggaagg caatgtgttt gtgttacgca agacttatca gaacgagcag
      361 gcagcaagac gcgcagcggc ggcaaagtgg cagcagctac aacgcggagc cgcagcgttc
      421 tccatcacac tggcacgtgg acgcgcagaa ctctaccccg aaatgcatgg cacggtaaca
      481 ggctttaaaa gcgagattga taatcaggac tggattattg cgaaagccga gcacaccatt
      541 gataacagcg gctttaccac gcagcttgag cttgaggcaa aaatcccgga atggatagca
      601 gaatgagact cttaaatgta ttagtccaga cacgtactag cgggctaaac ggcacagtct
      661 tatctgacca tctgccccta acacagagca gacggtcctt gatcttaccc actaataatg
      721 gacgcgcgac taatataatt tattgctttt tatttatacc tcactgagat ttagcgagct
      781 aatatattca aagaatttaa caattgagtg tcgagtgttt cagttgcagg aatcggtaat
      841 ccattcgcaa tgcggtaatt tttcacagac agccttgtgg atggccccat tatgccgtca
      901 atatttccgt tataaaatcc cctgtccagc aatgcgaact gaactcgcat aatcaatcgc
      961 ttacgtttct ctgcatcaga agcaagccca ttactcgtac ggttaacctc tgaagttcca
     1021 acagaactcg tgttagtggt tctattatta tcgttagaac gaagtgcacg caccgaggaa
     1081 gaggacgatg aggaacttgc acctgaactt gaagaaccag atgaacgtgg tgagctgtat
     1141 gtttttggat aatagggggt acttccacca tagtatccac cacctgatga ggatctatga
     1201 gagctatggc tcctatgaga gctatggctt cgatgccctg cgatataaaa tgggacttcc
     1261 gtgttaagtg gagctatcac taaatcatgc tcattaagag tcataccagg taaatcactc
     1321 gctcctgtag atgaatcgct tgcccataca gaattgttga gagccaaaaa tcccggaagt
     1381 agagcagcaa aattaaactt tttcataaaa ttggtctcgg tgattgatga aaacggcctt
     1441 ttgaggaact gaaatagcca gcacaggaag gcttctgagt acattcatca cattcttttg
     1501 gatagtaatt tttccaatca gagatcgact gaacagcaag ctccctagcc ctttcaggaa
     1561 gataacacaa aggataatta aaaattgtca gaggtatgcc tgacctgtat gcagcgtcta
     1621 tggcagagat tattttctca ctataactac tgtgatcaat gaagatggtg gaccagtttt
     1681 ttcgtgccca accgataggc tccaacccca taagggaaat ctgattgatg ttggagaaca
     1741 cacggccagc gaattctaca atatcatcca attcggtata gttagccagt gtaggaataa
     1801 ctctaagttc gatattaatc cccaagtttc ctgcattgat taacccttta accgtttcat
     1861 taaatgcgcc atcactccct accagatgat catgcacaag tggtcttgat gagtagagcg
     1921 ggataccaaa ggtgattttg atctttttgc ttcgctttgc catttcctgt gtaaagttga
     1981 tatcagcaaa tttgcgtccg tttgttaaaa catgtaaagc agtatctggt gaattctcga
     2041 tgataaaatc aatgaagtga aggaagtcat ccccatatag caaaggttca ccaccgctga
     2101 ccccaacaac tccgttcaag ccaaatgaag ctatagcaag agctgattgg gtaagtagcc
     2161 agtcatcatt tattttcttt ggtggctgcg aacaaaacag acacaagtta ttgcagcgtt
     2221 ccgtaactaa gacagtatta tgattggctt tgcgtgacag aataacgcgt atcatattgc
     2281 cattgttgat tatcccaata tctccatctt caattgactc aaataattca gtgctaataa
     2341 cagagaaatc aaagcatggt ggaatggcat cattattgcc ttcagcaact actagcaaat
     2401 tgggcaaaaa gaataaaggg ttcactggtt tttgtttgca taaccgatag aaccccgtgg
     2461 gcacgttctc tgtagaagca aaatgaaaga tatcgttcct gataacctca gacatatgcc
     2521 catcctttta gcatctcagc cttcgaacca ccctctgaga tgcagttcat cagatacctg
     2581 aacatcccct tgtgatattg acaaaacgtt gaacgactcc tatcaccgac aggctcaccc
     2641 tgtacactta tgttctggca tggatctgaa ccacaaaatg gttgatatgc acatgtatca
     2701 caacctggca gagcaaaatt gaacgatgaa ctcaatactg cgttatagta aggactatca
     2761 gaaaatgata gcgtcttaac ttcacccgca ctgaactccg tttcaggatt caccttctgc
     2821 aacatacggc tctcatcgct accatatact cgaccgtcat aattgaaaag aatactgtta
     2881 agcacgactc cacttggaga ctttaaatcc gcataaccac taaaaccggg attgaagatc
     2941 cttttcagat gaatggaggc cgaatgttcc acaaccctta tcccttcatt attaatctta
     3001 aggatttcat ctatcaacga ggcatagaag ctgaagtatt cgtccataga aaaggtgaaa
     3061 gaagcttttt gtgcaaaccc atagggacta acaggtcgga taaacatgtc cttgagacca
     3121 agagacaagt gagcctgaac aatcgattca ggacgtttga taagctcttt agtaactgta
     3181 gtaaccgttg caacgcggcc agcacctaaa gagtgtttga tagcctctac ccctgacact
     3241 gttctactgt acgcaagccc acgaccaaga attcgattac tgttgtgaac aacttcctca
     3301 ccatcaagcg acacagagaa cgttatgttc cggtctctcg accattcaat catatcctca
     3361 ttcaagacgg acagacttga tgccaccacc atctcaaaat tggttcttcc cagcgtttca
     3421 gcacattgat catagatgct ctgaattaaa tcaaaccgca agagaggctc cccaccctgt
     3481 atctctattt tgtaaggtgg cgttgatagt tttttaatag ccgaaataat atcggggatg
     3541 agctcaggat ttaggtcgta acctgaggca ttaacagaag ctctactaac ctggcaatac
     3601 ttgcatgtat ggtcacaacg taatgtgggt acaatcatga aaattggcct gacagccagt
     3661 tcattcatca atcgttttgc aaaagcagaa cttaaagcat agggagttat agcagatgag
     3721 ctttcactgg ttatgaaaag cttactttct agcacgtttg attgctccga actaattcgc
     3781 tcatcagaaa gatcaataag gtcttgctca ccaatgaaat gatgaaaacc tgcaaggttg
     3841 cttataaaca cctgtccatt aggcaatctg tcaaaattaa atggcatcaa ttccattatg
     3901 aaagcctctc atcaacactg cgaagcactt tatggacaat agagtctctc aacgcccctg
     3961 ttttatgctg tagcttttct cgcaaagagt aatcattaag cagcctatcg aattcataaa
     4021 ggaatttggg attatcgttt tcaaaagaga ttatccagtg ggaaacgtct tcatggagtt
     4081 tccatcgagt aagcggagtc atccaatata gactattgcg tataacccat tcagaatata
     4141 gttctttctc aaacactctt tcgctcattg caattatctt ccaatacaaa tattcatgat
     4201 cagcaaccac tttaaagcgg atttttccta tggcaacaat aatataacat atccatagat
     4261 atgttacaga tgccttgttt cccgttgact aatacatcac aacgttagta atggctatca
     4321 caattgtgaa cttccacttt tcacacaaga caagctgtta attggaatct gtatcagtac
     4381 aacttagaat agaggaagca ccacagtaag ggaggtcgct atgttccgtt gtccactttg
     4441 tggcgcatct gcccgtatcc gcactagtcg tccggaaaat gattcaaaca ctgtgcggca
     4501 aaagtattac caatgtaaca acctagaatg cggcgtatgc ttctcaacac tggaagcttt
     4561 ccataaattc acatcgaaac acgcctccgg cgctcactct tcagaaggta tcccgtggca
     4621 tgaactgcca gcttcacaca aaggaaacaa tcagatgagt ttgcccttac ctcaaaatta
     4681 acaggcagaa ttgccggagt aacaaaaaag agatagatta cgcgcgggtg cctttcggct
     4741 gatggtcgga gggaatacct gaaggccaga tgtggaaagg ccccggaaaa catttctgtt
     4801 taaccgaggc cctaaccgca ttaccttgac aagtgaaagg ttagcgcctc tccggaaaag
     4861 gagcaagtgc tatgtcgcaa aaatcgctta cggccatcac gttctgcgtg acggcaatcc
     4921 tcatcatctg gatgttgcac ggttcgctgt gcgaaatacg gatgagcttc tggggagcgg
     4981 agtttgcggc gttcttacag tgtaagcagt aaggaaaccg cgacggggga gcaatccccc
     5041 gtcaatcggt tgccagggta aggtcgataa ggcaccctat ctcacagaca tgaacaacaa
     5101 acccgcagcg taaaaactgc gggttttctt tttggtgtcc ttactgactt gaaacggtga
     5161 tcagactata agacaaagcc cacaatgtag cgggcttact cccctctccc tccactgaat
     5221 gtggacacat ggtggaacaa agagatataa aacattataa atcaatcggt tattgaaaga
     5281 aaaaaggcca cagagcggcc tttttagtta gatcagatta ctcgtctttg ggcgaagcgt
     5341 tttcgacccg gctttttaac ttctgcccgg gtctgaaggt caccacgcgc cgtgctgtaa
     5401 tgggaatatc ctcgcccgtt ttcgggttac gtcccgggcg ttgattctta tcacgcagat
     5461 cgaagttacc aaaaccagag agtttcacct gttcgccgtt ttccagagcg cgacggatct
     5521 cttcgaaaaa cagttcaacc agttctttgg catcccgctt gctaagccca agcttatcaa
     5581 acagatattc tgacatttca gcttttgtaa gcgccatagg ttcaatccct caatgatgcc
     5641 tggaatcgct cttttaatgc ctctacacat ttggcgacgg tagcggcaat ctcctcttct
     5701 tcgagtgtac ggctggtatc ttgcaggatc aggcttatgg cgaggctctt atacccctcc
     5761 gcaacaccct taccgcggta cacgtcaaat aagtttacgc caactacctg atttacgcca
     5821 actttcttac attcggataa aatatccgct gcgggaacgt tttctgcgac caccaccgcg
     5881 atgtcacgac ggttcgccgg gaagcgagaa atctcgcgcg cctgaggcac cacgcggtct
     5941 gcgagcttgt tccactccag ttcgaacacc agagtgcgac cgttaagatc cagtttacgt
     6001 tccagttcag gatgaacaac cccaacaaaa ccaatacgtt cacctttcag ataaatcgct
     6061 gcggattgcc ccggatgcag tgccggattc gcttctgcac ggaactcaac ctcattcagt
     6121 ttaccggtca ggtcgagaac ggattcaaga tcgcctttca aatcatagaa atcaacggtc
     6181 tcttttgcca ggttccagtg ctcttcgtaa cggttaccgc aaatcacacc ggctaacatc
     6241 agatcctgac gaatgcccaa cggtgcctga gtatctggta cgaaacgcag accgctttcg
     6301 aaaatgcgca cacggttctg ctgacggttc tggttgtaca ccacggttgc cagcaggccg
     6361 gtccacagag aaagacgcat tgctgacatt tcaacagaga tcgggcttgg cagcagtaag
     6421 gcttcaacgc ctggatggat catctgctgc actttcggat caacgaagct gtaggtgatc
     6481 acttcctgat agcctttgtc gttgagcagc gttttcacgc gcttgagcga caggtcagct
     6541 tcacggtgag tacccataat caggcttgcc tgtaccggct catccgggat gttgttgtag
     6601 ccgtaaacac gcgcgacttc ttcgaccaga tcttcttcaa tctccatatc gaaacgccag
     6661 ctcggcgcaa ctgcctgcca ctcgtctttg ccttcggtca cttcgcagcc gagacgacgc
     6721 agaatgtcag ttacctgctc atccgcaata tgatggccga tcaggcgatc cagtttgcta
     6781 cgacgtaaag tgatggttgc acgcttcggc agcgttgctt cgttggtgat atcaattacc
     6841 ggaccagcct caccaccgca gatgtcgatc agcagacggg tcgcacgttc catcgctttg
     6901 tgctgcagtg ccggatcaac gccacgctca taacggtgag acgcatcagt atgcaggcca
     6961 tgacgacgag cacgaccggt gatagacagc gggctgaaga aagcgcattc cagcaagacg
     7021 ttttgcgttt cgtcattcac gccagagtgt tcgccaccaa agatgccgcc catcgccagc
     7081 gccttgttgt ggtcggcgat gaccagagtg tcagcattca gcttcgcttc agtgccatca
     7141 agcagtacca acgtttcgcc ctctttcgcc atccgcacca caatgccgcc ttcaatgcga
     7201 tctttatcga aagcgtgcat cggctggccc agttcgagca gtacatagtt ggtgacgtca
     7261 acaactgcat cgatagaacg gatcccgcaa cgacgcagtt tttctttcat ccacagcgga
     7321 gttggcgctt taacgttaat gccttttacc acacggccaa gataacgcgg gcaggcttcc
     7381 ggcgcttcga ctgtaatcgg cagcgtgtcg tcgatggtcg caccaaccgg aacgatttcc
     7441 ggttcaacca gcggcagctg attcagcacg gcaacgtcac gcgcaacacc aatgatacct
     7501 aaacagtcgg cacggtttgg cgtcacgctg atttcgatgg tgttgtcatc aagtttcagg
     7561 tattcacgga tgtcggtgcc aatcggcgca tccgcaggca gctcgataat gccgctatga
     7621 tcgtcagaaa ttcccagctc agaaaaggag cacagcatcc cttcagacgg ttcaccacgc
     7681 agtttcgccg ctttaatttt gaaatcaccc ggcagaacag caccaatggt agctaccgcc
     7741 acacgcaggc cctgacggca gtttggcgca ccgcagacga tgtccagcag gcgatcgccg
     7801 ccaacattca cttttgtcac acgcagtttg tcagcgttcg gatgctgcgc acactcaacc
     7861 acttcaccaa cgaccacgcc gtggaagcta ccggcaaccg gttctacacc gtcaacttcc
     7921 aggcccgcca tagtgatttg attcgccagc gcatcgctat caatcgccgg gttcacccat
     7981 tcgcgtaacc acagttcact gaatttcata atctattcct gccttattta aactgtttga
     8041 ggaaacgcag atcattttcg aagaatgaac gcaggtcggt gacgccgtaa cgcaacatag
     8101 tcagacgctc catccccatc ccgaaggcga aaccagagta aacttccggg tcgatgccaa
     8161 cgttacgcag cacgttcgga tgcaccatcc cgcaacccag cacttccagc catttaccgt
     8221 ttttacccat gacgtcaact tctgcagaag gctcggtgaa cgggaagtag gaaggacgga
     8281 agcgaatctg caaatcttcc tcaaagaagt tacgcaggaa atcgtgcaga gtgcctttca
     8341 ggttggtaaa gctgatgttg gtatcaacaa tcagaccttc catctgatgg aacatcggcg
     8401 tgtgagtctg gtcgtagtcg ttacgataaa cgcggccagg cgcaatgata cgaatcggtg
     8461 gctgctgggc tttcatggtg cggatctgta cgccagaggt ctgggtacgc agcaggcggg
     8521 tagtgtcaaa ccagaaagtg tcgtggtcag cgcgcgccgg gtggtgacca ggaatgttca
     8581 gagcatcgaa gttatgataa tcgtcttcga tttccggccc ggttgccacg gtaaagccaa
     8641 gctcaccgaa gaaactttcg atacggtcga tagtacgggt aaccggatgc agaccgccgt
     8701 tttcaatgcg acgacctggc agagagacat caatcgtttc cgccgccaga cgcgcattca
     8761 gtgcagcgct ttccagttcc gctttacgcg cattcagcgc ctgctgaacc tgctctttcg
     8821 cttcgttgat aaccgcacca gctgccggac gctcttctgg cggcagctca cgcagggtcg
     8881 tcatctgaag ggttaagtgc ccttttttac ccaaatattc gacgcgcaca ttatctaacg
     8941 cggcaacatc tgacgcctgg ctaatggccg ccttcgcact ggcaaccagt tctgcgagat
     9001 gtgacatggt tttcctcatt gtgtcagtgg tgacactggt tcgttggact tagagcctat
     9061 cccatcaggc tattttactt gccattttgg tcccgggcag tgctcgaaat tctcacgtac
     9121 ttaaatacgc tccggtttct ccgcgctggc cgtgtccaga ctggctgcga caattacacc
     9181 tgatgagaca ggctttttat ttttcaaaac gcgcatacaa aaaaagcctc cactgggagg
     9241 ctttcaggcg ctgttttccg tttctcttct cacgcgctag cctcctggat tcaggtgcta
     9301 aagtaaaaaa agaagcggaa aatagcagca ttcattgctt gcgttacctt ttggtactct
     9361 tcaaaagacc tttattgaaa aggctacggc gataaaagtc aatgttttga tggcgttgaa
     9421 acgaaaagag ggagactagc tccctctttc aactggctta tgccagagct gctttcgctt
     9481 tttcaaccag agcggtgaac gctactttgt cgaatactgc gatatcagcc aggatcttac
     9541 ggtcgatttc aacagaggct tttttcaggc cattgatgaa tttgctgtaa gaaataccgt
     9601 tctgacgtgc tgctgcgttg atacgcgcaa tccacagttg acggaactga cgcttacgtt
     9661 gacgacggtc acggtaagca tactgaccag ctttgataac agcctggaag gcaacgcggt
     9721 atacgcgaga acgcgcaccg tagtagcctt tagcttgttt caaaattttc ttgtgacgtg
     9781 cgcgtgcaat aacaccacgt tttacgcgag ccatatgtgc tctcctgtat ctatattcta
     9841 attaaaaagt taaaaacgtt aacggcttat gcgtacggca ggcacgcgat taccaggccc
     9901 agatcgcctt tggaaaccat ggctttcgga cgcaggtgac gtttacgttt ggtcgctttt
     9961 ttggtcagaa tgtgacgcag gttagcgtgc ttgtgcttaa aaccaccttt accggttttt
    10021 ttgaagcgct tagcagcacc gcgtacggtc ttaatttttg gcattttaat aacttccact
    10081 tcgcattgtt aataaacgaa acaaaggcga acaaagcctg tgaagcccga aggctccaca
    10141 gacagtgcta cttgaaggcc ttactgtttc ttcttaggag cgagcaccat gatcatctga
    10201 cggccttcga tcttcgttgg gaaggattcg accactgcca gttcttgcaa atcgtctttc
    10261 acgcgattaa gcacttccat accgatttgc tggtgcgcca tctcacgacc gcggaaacgc
    10321 agcgtgattt tggctttatc accctcttcg agaaagcgaa tcaggctgcg gagttttacc
    10381 tgatagtcgc cttcatctgt accaggacgg aatttaattt ccttaacctg gataactttt
    10441 tgctttttct tctgttcctt agaagacttg ctcttttcat agaggaattt gccgtaatcc
    10501 attatacgac aaaccggcgg ctcggcgtta gggctgatct cgactaagtc tactccggct
    10561 tcttctgctt tctccagagc ttctctcaga ctcacaatac caagctgctc gccttccaga
    10621 cctgttaagc gaacttcctg ggcgcgaatt tcgccattga tacggttagg gcgcgccgtt
    10681 tgaactcgtt ttccgccttt aataccttat tcctccaatt gtttaagact gcggctgcga
    10741 atctcttgtt gcagcttctc gatcacttca tttacgtcca tgcttcccag gtctttacca
    10801 cggcgggtgc gaacggcaac tttgcctgat tccacctctt tatcaccaca gaccagcata
    10861 tatgggacgc gacgcaaagt gtgctcgcgg attttaaagc caatcttctc atttctcaag
    10921 tctgctttaa cacgaatgcc cgcatttgat agtttttgcg tcaattcgtt aacgtattca
    10981 gactgtgaat cggtaatatt catgataaca acctgaactg gcgcaagcca ggtcgggaag
    11041 aaaccagcga attcttcggt caggataccg atgaaacgtt ccatcgatcc cagaattgcg
    11101 cggtgaatca ttaccggtac tttacgttca ttgtcttcgc ctacataaga agcgctcaga
    11161 cgagaaggca aagagaagtc cagctgtact gtaccgcact gccatgcacg atcgaggcag
    11221 tcatacaggg taaattcaat tttcggaccg tagaaagcgc cttcacccag ttgatattca
    11281 aacgggatgt tgttttcttc cagcgcaacc gccaggtccg cctcagcacg atcccacatt
    11341 tcatcactgc caatacgttt ttcaggacga gtggagagtt tgacgacgat cttctcgaag
    11401 ccaaaagtgc tgtacatatc atagactaaa cggatacatc cgttaacttc atcgcgaatt
    11461 tgttcttcag tacagaagat atgcgcgtca tcctgggtaa atccacgcac gcgcatcagg
    11521 ccatgcagcg aacctgacgg ctcattacgg tggcagctac caaactcggc catacgcagc
    11581 ggcagatcgc gataagactt cagcccctgg ttgaaaattt gtacgtgacc cgggcagttc
    11641 atcggcttaa tgcagtattc acggttctca gaagacgtgg tgaacattgc atctttgtag
    11701 ttgtcccagt gaccggtttt ttcccacagg acacggtcca tcatgaacgg acctttaact
    11761 tcctgatact ggtactcttt cagtttagaa cgaacaaaca cttctagttc acggaagatg
    11821 gtccagccgt cgttgtgcca gaataccata cccggcgctt cttcctgcat atggtacagg
    11881 tcgagctgtt taccgatttt acggtggtcg cgtttcgcgg cttcttccag gcgctgcagg
    11941 taagcgttaa gcgctttttt gtctgcccac gccgtaccgt aaatacgttg caacattttg
    12001 ttgttgctgt cgccacgcca gtaagcccct gccgttttca ttagtttgaa atgatggcag
    12061 aaacgcatgt tcggtacgtg cggaccgcgg cacatatcga catattcttc atggaagtac
    12121 agacctggct tgtcatcatg ggcgatgttt tcgtcaagaa tggagacttt gtagctctcc
    12181 ccacggttgg cgaaagtttc acgcgcttcg tgccagctga ctttcttctt aatgacgtcg
    12241 tagtttttct cagcaagctc atgcatccgc ttctcgagtg cttcgacatc ttcctgggtt
    12301 aacgtgcggt caagatcaac gtcgtaataa aaaccgttgt caataaccgg gccgattgcc
    12361 attttggtat gcggccaaag ttgtttaatc gcgtgcccta acaggtgcgc acaggagtga
    12421 cgaatgatct ccagaccgtc ttcgtctttg gcggtaatga tggacagttg tgcgtcgttt
    12481 tcaatcagat cgcaagcatc aaccagttcg ccattaacgc gccctgcgat acaggctttc
    12541 gccagacctg gaccaatgtc cagcgcaaca tccatggggc ttacagcgtg gtcgtaatgg
    12601 cgttggctgc catcaggaag agttataaca ggcattttat atccttattt gcagtggtga
    12661 cccacacgaa agatcacata caaagaaaaa tttgtttatt aacagttcat tgcgaaacca
    12721 tctagccaac aaatgctaaa ttggttcgca atcaggtaca caaatgaaag acattaacct
    12781 ctcaccttcc acccggttga gcgatgttaa cactaaaaaa agggagattg tacctttccg
    12841 tttcacattg attcgattcg caattcgttt gtagtgagta caccagcaaa acgcttaaag
    12901 aaccactgat aacatcacgg tgcgtctgaa aggtttactg cgagcggatt tacagcagca
    12961 taaactgaat ccagcaaagt tcgttgcgtg atttggcggt agtattttaa acgctcatga
    13021 gattaatgac gaagtggtca tatcacaatg ataaaagtga cacaattctt ataacaattt
    13081 ttcgtgcaca tttcgttctg gcgataataa ttaatcatca tcctcaaaac ctccagatat
    13141 atataaggcg aatagattat gagtcaaaac gatatcatta tcagaactca ttataagtct
    13201 cctcatagat tgcacatcga tagcgacata ccaacacctt catcagagcc tattaataaa
    13261 tttgcgcgcc agctcatcac cctacttgat acctctgact taagttcgat gctgtcatac
    13321 tgtgttactc aggaatttac cgcaaactgt cgaaaaatat cacaaaattg ttattccact
    13381 gcccttttta ccattaacct tgccacttca cccatccatg cagaaaatat actcattaca
    13441 ttacactata aaaaagatat catttcctta ttactggaaa ccacgcctat taaagctaac
    13501 catttgcgaa gcatactgga ttatattgaa caggaacagt taactgctga agatcgtaac
    13561 cattgtatga aactgtctaa aaaaatccat agagaaaaaa ctatacaccc aacagtaaat
    13621 ctcaatggta gtgcatgttt tttgcaatct ccttctgacg ctattttttg tcgccatctg
    13681 tcattgcaat acgcccttga ttcattgaga aatggaaaag gcaaagtcaa cctgattaaa
    13741 cattactcct ccgttgaatc catacagcag catgtcccct tagtccggga cgcggagttc
    13801 agagcattac ttcgccatcc tcctgcaggg agtcgcgtta tcgcgagtaa ggattttggc
    13861 ttcgctttag attttttctt ttgtcgaatg atggcaaaca atatcagtca tatgtccgcg
    13921 attttatata tagacaatca tactttgtca gtaaggctac gaataaagca gtcagtgtat
    13981 ggacaattaa actatgttgt gtccgtttac gacccgaacg ataccaacgt tgccgtcaga
    14041 gacacccaca ggacagcacg gggctttctt tcgcttgata agttcatcag ttcaggtccc
    14101 gatgctcaga cctgggctga taggtatgtt cgcaactgtg caattgctat tctgccccta
    14161 ttacctgtgg gagttccagg ggctattttc gcgggtattg catcacgaat gccatttgcc
    14221 cctatacatc catcggcaat gttgttaata atggctacag gccagactca acagcttatt
    14281 acattattca aacagttgcc catactccct gaaaaagaaa tcattgaaat aataactgcg
    14341 cagaatagcg ttggtacacc tgctttattt ttggctatga tgaacggaca tactgacaac
    14401 gtgaaaatat ttatgcaaga aattcagtca ctggtagata atcacatcat tcatgaagat
    14461 aacctggtta aattactgca aactaaaagt gctaacgaaa cacctggact ctatatctcc
    14521 atgttgtatg gattcgatga aataatcgat atctttctga atgcattaac cactccaata
    14581 gcacaagaac ttttaaacaa aaaaatggtg atggatattt tagccatgaa aacacgtgat
    14641 ggtgaaccag ggttatttgc tgcaatggaa aataatcacc ctttgtgtgt cacacggttc
    14701 ctctctaaag tttatgggat cgccgttaaa tataaactca gcaaaatcaa catcatggac
    14761 ttattaaaag gcgcgacagc acagggaacc cctgcattat acatcgccat gagcaagggt
    14821 aatgaagacg tcgtgttatc ttatatatcg acgctgggtg cttttgcaaa aaaaacattc
    14881 ttttagtcaa catcagttat ttacactatt ggccgctaaa aatcatgaca acatgtcagc
    14941 tgttcatata gccattcatc ataagcatta taaaactgta gaaacatatt atgctgctat
    15001 aaatgtcatc agccaaagcc tgagttttag tgctgatgaa ataaagacgt atttataacc
    15061 agctatttac gatatatatc gatacataat tttatttttt tcataaaacg cagaaacagt
    15121 gaccaggtgt acaacgccac ccaacggctg taataacaaa aaatcccttg atgcccgtcc
    15181 cttttgttac actccgttat cacgcacaag agatatgcag gacactggta tgccgactaa
    15241 acgctttgat aaaaaacact ggaagatggt ggtggtgcta ctggcaatct gtggcgctat
    15301 gttgttgcta cgttgggcag caatgatttg gggctgagca gtgacaaacg aagacagcca
    15361 gaccgcataa cacggtccgg cagataattt tacatgctgt agcccagcga taaggttgtc
    15421 cgacggtcgg tatgttccgg tgcagactct ggaggttcag agttccatgt gacgttgtaa
    15481 gccaccttca gtccaaaatg ttcattaata gcaacattta atgcgctttc ggagttcagc
    15541 gttgtgtctt ccgcgccaaa gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac
    15601 tgccatgcat aagccccgga ggcatagccc agcggctggg tttcactggc attgtcggta
    15661 tatttgtcgt aacgcacacc tggaccgaat tcaaagcgga aactgtgtac cgggccattc
    15721 aaaaactgat gaccataacc cgcggtcaac acatcgcgct ctcgatagcc gttataacgg
    15781 tctgtcagcc agcttgcctg accaaataaa tagtcataat cagttaaatt aaaacggcta
    15841 cgcccgcccg ccgcatattt ttctgaagaa cgctcatcat tagaagaagt attactggcg
    15901 ttcccccaca gcgaccaggc agtggtttgt ccataccagg tcatggtggt gtcagccgta
    15961 agtgaggagc ttttcgtatt gcctgattgt gcaagatatc ctgcgttcag attaccttcg
    16021 aaaggttttt tagcgctggc aggatcgtcc atgacagtaa aaacggaatc atcggcagct
    16081 gcattcagtg acgcaaacat gccccccgcc aacataacga tggcaggaac tgtcttcaaa
    16141 agcttcattt atcaagagtc cgtacaacaa aaaaagagac catcgcggtc ccggaaactt
    16201 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
    16261 acttagcaat gtattcttat ttcatttttt gaataag
//
LOCUS       DAZFKM010000108        16197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFKM010000108 DAZFKM010000000
VERSION     DAZFKM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..184)
                     /locus_tag="ACHKAY_003652"
     CDS             complement(<1..184)
                     /locus_tag="ACHKAY_003652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702261.1"
                     /translation="MTKNINFSRFALSPYITGNSGDLLASIFMPHLPSGVASDSQYVR
                     VEKSDYFHWVIYRHKQI"
     gene            complement(338..1297)
                     /gene="nrdF"
                     /locus_tag="ACHKAY_003653"
     CDS             complement(338..1297)
                     /gene="nrdF"
                     /locus_tag="ACHKAY_003653"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5702262.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1307..3451)
                     /gene="nrdE"
                     /locus_tag="ACHKAY_003654"
     CDS             complement(1307..3451)
                     /gene="nrdE"
                     /locus_tag="ACHKAY_003654"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5702263.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3424..3834)
                     /gene="nrdI"
                     /locus_tag="ACHKAY_003655"
     CDS             complement(3424..3834)
                     /gene="nrdI"
                     /locus_tag="ACHKAY_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO5702264.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3831..4076)
                     /gene="nrdH"
                     /locus_tag="ACHKAY_003656"
     CDS             complement(3831..4076)
                     /gene="nrdH"
                     /locus_tag="ACHKAY_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO5702265.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4325..4654)
                     /gene="ygaM"
                     /locus_tag="ACHKAY_003657"
     CDS             complement(4325..4654)
                     /gene="ygaM"
                     /locus_tag="ACHKAY_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO5702266.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4806..5150
                     /gene="ygaC"
                     /locus_tag="ACHKAY_003658"
     CDS             4806..5150
                     /gene="ygaC"
                     /locus_tag="ACHKAY_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO5702267.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5187..5636)
                     /gene="alaE"
                     /locus_tag="ACHKAY_003659"
     CDS             complement(5187..5636)
                     /gene="alaE"
                     /locus_tag="ACHKAY_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO5702268.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            6304..6708
                     /gene="stpA"
                     /locus_tag="ACHKAY_003660"
     CDS             6304..6708
                     /gene="stpA"
                     /locus_tag="ACHKAY_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO5702269.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(6755..7279)
                     /gene="ygaP"
                     /locus_tag="ACHKAY_003661"
     CDS             complement(6755..7279)
                     /gene="ygaP"
                     /locus_tag="ACHKAY_003661"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO5702270.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(7289..7588)
                     /gene="ygaV"
                     /locus_tag="ACHKAY_003662"
     CDS             complement(7289..7588)
                     /gene="ygaV"
                     /locus_tag="ACHKAY_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO5702271.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            7771..7929
                     /gene="yqaE"
                     /locus_tag="ACHKAY_003663"
     CDS             7771..7929
                     /gene="yqaE"
                     /locus_tag="ACHKAY_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO5702272.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8013..8462
                     /gene="kbp"
                     /locus_tag="ACHKAY_003664"
     CDS             8013..8462
                     /gene="kbp"
                     /locus_tag="ACHKAY_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO5702273.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8463..9125)
                     /gene="csiR"
                     /locus_tag="ACHKAY_003665"
     CDS             complement(8463..9125)
                     /gene="csiR"
                     /locus_tag="ACHKAY_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO5702274.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9146..10546)
                     /gene="gabP"
                     /locus_tag="ACHKAY_003666"
     CDS             complement(9146..10546)
                     /gene="gabP"
                     /locus_tag="ACHKAY_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO5702275.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            complement(10857..12137)
                     /gene="gabT"
                     /locus_tag="ACHKAY_003667"
     CDS             complement(10857..12137)
                     /gene="gabT"
                     /locus_tag="ACHKAY_003667"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO5702276.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            complement(12151..13599)
                     /gene="gabD"
                     /locus_tag="ACHKAY_003668"
     CDS             complement(12151..13599)
                     /gene="gabD"
                     /locus_tag="ACHKAY_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5702277.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(13622..14890)
                     /gene="lhgO"
                     /locus_tag="ACHKAY_003669"
     CDS             complement(13622..14890)
                     /gene="lhgO"
                     /locus_tag="ACHKAY_003669"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO5702278.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(14910..15887)
                     /gene="glaH"
                     /locus_tag="ACHKAY_003670"
     CDS             complement(14910..15887)
                     /gene="glaH"
                     /locus_tag="ACHKAY_003670"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO5702279.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
BASE COUNT         4007 a         4203 c         4086 g         3901 t
ORIGIN      
        1 ttatttgttt atgtcgatat attacccaat ggaaatagtc acttttttct accctaacat
       61 actgagaatc tgaggcaacc cctgagggca aatgtggcat gaatattgat gcgagcaaat
      121 cgccggaatt tcccgtgata taagggctga gagcaaatcg agaaaagttg atattttttg
      181 tcatagatag cgaaaaaacg cctgaatatt gtagtttcag gcgaataatg caacagagta
      241 tcagtgtaga tcactacaga atatttgcgt gataaaaagc tatttggcgg gaattatttc
      301 cctgctgcgg gtagtgatat ttttgaaaat aacaccctca gaaattccag tcttcatctt
      361 ctgtttcaac cgctttcccc atcacataag aggagcctga accggaaaag aaatcgtgat
      421 tttcatcggc attcggcgaa agcgcggcga ggattgccgg attcacttcc gccatttctg
      481 cgggaaataa cggttcgtag cccagattca tcaaagcctt attggcgttg taacagagaa
      541 acgctttcac atcgtcagcc cacggggttt cggcgtacag ctcatcggtg tattgcaact
      601 cgttgtcgta gagttccagc aacaaatcga aggcgaaact cttcaactct tcacgttgtc
      661 ccagagatat cttttccatg tttttctgat atttatagcc tatgtagtaa ccgtggactg
      721 cttcatcgcg gataatcaga cggatcagat ctgcggtatt ggtcagcttt ccgcggctgg
      781 aaaaatacat cggcagccag aaaccggaat agaacaaaaa agattcaaga aacacactgg
      841 cgattttctt tttcagcgga tcatcaccgc gataatgttg ctgaataatc tgagcttttc
      901 gctgcaacgg tgcgttttct tcactccagg cgtaggcggc atcgacatct ttggtctgac
      961 acagcgtcga gaaaatcgaa ctgtaagagc gggcatgaac cgcttccata aagctgatat
     1021 tcgataatac tgcctcttca tgaggcgtga gcgcatcggg catcagcgaa ggcgcgccga
     1081 taacattttg cagcgtgtcg agcagcgtca ggccagtaaa aacgcgcatt gtcagttgtt
     1141 gttctacgac acttaatgtc tgccatgcag gtatatcgtt cgacagtggc accttttctg
     1201 gtagccagaa attgctggtc aggcgattcc acacctccag atctttatcg tcagatatct
     1261 tgttccagtt gatggcgctg atacgtgaga gtttcataga tattccttaa agtgcacagg
     1321 agacgcagcc ttcaatttca gtgccttcca gcgccatctg acgcaggcga atgtaataga
     1381 gcgttttgat acctttgcgc caggcgtaaa tctgcgcttt gttgatatcg cgagtggtgg
     1441 cggtatcggg gaaaaacagc gtcagcgaca aaccctgatc gacatggcga gtcgcttccg
     1501 cgtaggtgtc gatgatcttt tctgcgccga tttcgtaagc atcctgatac agcgccagat
     1561 tctcgttagt cataaacggg gcagggtagt aaacgcgtcc tgttttgccc tctttgcgta
     1621 tctctacttt cgccacaatc ggatgaatac tcgacgtagc atggttgata taagagatag
     1681 aaccggttgg cggcaccgcc tggagattct ggttgtatat gccgtagcgc atcacgtcgt
     1741 cgcgcagttg cgcccacatc tcacgggtag gtaacgtaat accgctgcgg gcaaacagtt
     1801 cgccaacttt cgccgttttc ggctgccagt taccttgcag atattggctg aaatattcac
     1861 cactggcata gcgtgactgt ttgaacccgg cgaaggtttc accgcgttcg cgcgccagca
     1921 acatcgaggt acgcagtgcg tgccaggtga tggtatagaa atagagattg gtgaaatcca
     1981 gggcttccgg cgaaccataa gcgatgcctt ccctcgccag ataaccatgt aaattcatct
     2041 gccccagtcc gatggcgtgc gaggcggcat ttccggcttc gatggacggc acgctgcgga
     2101 tatgactcat atctgacacc gccgttaaac cgcgtacggc agtctctacc gtgcgggcaa
     2161 agttggggga atccatggtg tgggcaatat tcaacgaacc taaattgcag gaaatatcat
     2221 ggcctgtacg ggcatagtcg agattctcgt catactctga ggcgctgtta acctgcaaaa
     2281 tttctgagca gagattactc atatttatgc gcccggcgat agggttagca cggtttaccg
     2341 tgtcttcata catgatgtag ggatagccgg actcaaactg gatttctgcc agtcgctgga
     2401 agaaatcacg ggcgttgagg tattttttgc ggatgcgttc atcggcaacc agttcgtcat
     2461 agtgttcgct aatggcgata tcggcaaacg gcttgccata aactcgctca acgtcatagg
     2521 gcgaaaacag cgccatctgc gcattctctt ttgccagatg gaaagtgata tccgggatca
     2581 ccacgccaag cgacagtgtt ttaatgcgga ttttttcgtc ggcattttcc cgtttcgtgt
     2641 cgagaaaacg cagaatatcg ggatgatgag catgtaaata gactgcacca gccccctgac
     2701 gcgcgccgag ttggttggga taggaaaatg cgtcttccag cattttcatc accggaatta
     2761 cgccagaaga ttgattttca atacgtttaa ttggcgcgcc cgcttcccgc agattcgaca
     2821 gcaaaaatgc cacgccgccg ccgcgtttcg acagttgcag tgcggaattt accgcccggc
     2881 caatcgactc catattgtct tcaatacgca gcagaaaaca ggaaaccagt tcgccgcgct
     2941 gctgcttacc gcagttgagg aatgttggcg tggctggctg aaagcgtcct gacagcattt
     3001 catcggtcag ttgcaacgcc agcgtcgcat cgccttgtgc cagcgtcagc gccaccatcg
     3061 tgactcgatc ggcaaaatct tccagataac gtttaccgtc gaatgtcttc aacgtatagc
     3121 tggtgtaaaa cttccatgcc ccgaggaatg tctggaaacg aaaaccgctg gtgtgtgcgt
     3181 gggcaaacag cgtaatgaca aagtcgcgag agtagcgatt aagaacgctt tcatcatagt
     3241 aaccttcgtt gaccagccag ttcaggcgct gctgctggct actgaaggtc acactgttcg
     3301 gacgcacatg cgtcgcaata aaggcgtcaa cggcctggcg gtctttatcg aactgaatgc
     3361 gacctgcgct atcgtagagg ttaagcatcg cattcagcgc gtggtaatcc atcgtttcct
     3421 gcgtcaggca ttctgcggtt gtcgttgcca aaattcggtt actcctttac gaacgttttc
     3481 gatatcgctt tgcgtaccca tgagttcaaa acggtacagc cacggcacgc cgcatttccg
     3541 ggcaatcaca tctccggcgc ggccatacgc ctcaccaaag ttgcgattac cagaagcaat
     3601 aacgccgcga agcaacgccc ggttgtgctc gtcgtttaaa aagcgaatta cctgtcgtgg
     3661 caccgcgcca gccgtaccgc cgccgccgta agagggcacg atcaggatgt aaggctcgtc
     3721 tacctgaatc cgttcccgct cattgagcgg gatgcgcacc gcgggcagac ctaaacgttc
     3781 gataaaacgc tgcgtgtttt cggagctgct ggagaagtag acgagctggc tcatgcactg
     3841 gccgcgtgtg gcgctggatg cagacggtta atcatgtccg gacggaaacc agaccagcta
     3901 agatcgccag caatcactac cggcaactga cgaaagccct gagcacgcaa cgcttccgct
     3961 gcttcaggaa cgcgatcgac attaatcatt tcaaaatcaa agccccggtt ttccatcgcc
     4021 cgtttggtgg cgtggcactg aacgcaatcg ttacgagtgt aaatagtaat gcgcatgatt
     4081 cgtatttccg tttaaaatga agatacggcg cgatgatacg cgtcgggttg tctctctgtt
     4141 gatacagaga tactagatgt agttgaaaaa aagattcaac cacacaatat atagcaaata
     4201 gtggtcgaaa ttaccctgga tatgagcgtg acggggtagg gggatttttg tgattcacca
     4261 ggcaaaaaga aaccccgaag ccaggcttcg gggtcaaaga cgcgtattta ttatcatttt
     4321 tgcactacga tttgcgcatg cttaacagtg cgccgataaa aatacctacc gcagctgctg
     4381 tacccacgct acaccagggt ctttcacgaa caaaagaatc cgcgcagcca acggcatcgc
     4441 gtgcggcttg ctggacgcga gtacgaccat gcattcgcgc cctggtttct ttcagcaatg
     4501 cctgagcttt actgcgtgcg gcttcagctt cccctttggc gtcgctgccc caggacttca
     4561 atacagattc gaggctgtca gctaattgat tgacatcatt ctggatatcc tgcacgccat
     4621 catcaacgtc gttgcggttc ggtcggttaa acatatgatc ccccattaga attcgatgta
     4681 attttcagtg tagaccataa ttttcttaac tgaggttcat cgcggcttta tggacttttc
     4741 tgaaagcgtt gtgaatgcgc aatcaggtaa ggtaggcccg cggttgataa taacgaggat
     4801 caaatatgta tttacgacca gacgaggtgg cgcgcgtact tgaaaaagtc ggttttactg
     4861 tcgatgtggt aacccagaaa gcgtatggtt accgccgtgg ggaaaattat gtctatgtta
     4921 atcgcgaagc gcgaatggga cgtaccgctc tggtaattca tccaacatta aaagaacgca
     4981 gttcaacact ggctgaaccc gcttccgata ttaaaacttg cgatcattac cagcaatttc
     5041 cgctctattt agcaggcgag cgacacgagc attacggtat cccgcatggc tttagttcgc
     5101 gtgttgcgct tgaacgttat ttgaatggtt tatttggcga agccagttaa agacgcgact
     5161 ggcgatgcca gtcgcgaaaa gaagagtcag gcttttacct gctggtaacg gctgacttta
     5221 aacagtcggc ggcaataatc gaggaagtag ccataaaccg cccccatcaa catcgaaaca
     5281 acgatgtttg aactgaccgc cgccataatc tgatgccagt ctgcgcccac cactaacaag
     5341 atcgccacat acaccggtga ctggaacgtc acataagcca ggatatcagc cagatttttt
     5401 atccagcccg acgggctaac tttgcgtgcc gcgcgcataa acagatcgcg gtacataccg
     5461 tatggccatg caattaagat gttcgccgga atcgctacca atctggaata aaaagactgt
     5521 tcgaagctca ttccggagag gaaaacttca atacacatgt tcacgacaga acagtaaaca
     5581 accatcgcga acgtatctgc aactgcatga cgcaagcgtg actgcggtga gaacatgcta
     5641 atgctcctta ataaaagcgt aatgggatgt taatggagat gaaagcagtg gatctcattg
     5701 ccatctgcat tttcgcatag cgtatatcgc tggctatgac tattcaatta gtgaaaaatt
     5761 tttatttagt cagtttttgt agataaaata ttccgtaacg gctcgttttt tgtatactgt
     5821 ggatttttac atgccgatat gatttatttt caattaaatc aattggatat gaccttgcct
     5881 ttaatttcac taatgaaagt ttgggggtga ggtaacgcta taagcgtaga gaattcggtg
     5941 tatgtcgtga ccgggtcatt tacagcattt tttatgccag aaaatgtaca ttaacagcag
     6001 aaagcaccag tgaggggtgt tttcttttat tctgttatat tgcattttta ttcacttcgg
     6061 ttttgtcagg cttgcggaat tagcgagcag agagcaccac tctgttcacc aataatctaa
     6121 attactcatc aaaatggcta atatataatc ttgaaattat tctgagagtt tcagaaaata
     6181 acagctgaaa taatctcgcg caggactgta aatagattaa attttgtgga aatataataa
     6241 gtgatcgctt acactacgcg acgaaatact tttttgtttt ggcgttaaaa ggttttcttt
     6301 attatgtccg taatgttaca aagtttaaat aacattcgca ctctccgtgc gatggctcgc
     6361 gaattctcca ttgacgttct tgaagaaatg ctcgaaaaat tcagggttgt cactaaagaa
     6421 agacgtgaag aagaagaaca gcagcagcgt gaactggctg agcgtcagga aaaaattagc
     6481 acctggctgg agctgatgaa ggctgacgga attaacccgg aagagttatt aggtaatagc
     6541 tctgctgctg caccacgcgc tggtaaaaaa cgccagccgc gtccggcgaa atataaattc
     6601 accgatgtta acggtgaaac taaaacctgg accggtcagg gccgtacacc gaagccaatt
     6661 gctcaggcgc tggcagaagg taaatctctc gacgatttcc tgatctaatt actgaggccg
     6721 gatgtcgctg ctagggcgcg tccggcgtcg cttctcaagt tcgttggttc cacggcatct
     6781 tatctaacaa ccttgccatt ccacaaaaac cgctgattcc tgcaaacagt aacccggcac
     6841 caacaaagcc acttaataag aagaaaccgc tatttacggt ataacccagt acaacgccga
     6901 ttaatattaa accacccgca gcgatctgca cctggcgcat taacggcaag ggttgagatt
     6961 tatttaccgc tactggcaat ccagctcttt tccagccatc aatcccatct tcgagtaaaa
     7021 atatttctgc gggggcggca atcgctgcta atttatcggc gttattactg gtgcgtttac
     7081 ctgcctggca gtggaatata atttgctcgc gacgtaattt aggtggaaga cctgactgtt
     7141 ccagcacgga taatggagcc agatctgctt caggaatatg ttcacgaaga tattcatcag
     7201 cgtcacgaat atcgattaac tttgcgccgc gtgcgattaa ttcttgtgca tcatgcggcg
     7261 aaatggttgt caaagccata gtgactcctt acggacaata gacatttttc agggtggcga
     7321 taatggcatt taccgcctca tttttaatgg aatatagaat gcgttgggca tcccgttggc
     7381 tgtcgataag cccttcgtcc cgcatacgag cgagatgctg tgatgtcgca gaggcactca
     7441 gtccggtaat gcgcgtcagc tctcccgcgc tggtgccggg ggaaccgcta agcatgcaca
     7501 gaatcagcaa tcgtttagga tggctcattg ctttcaataa ggccgctgcc tgttcggcac
     7561 tggcctgtaa ttgcgcgagt tcagtcatat ttatttaagt attttctaaa ttaagtaaac
     7621 tctaaactaa aaatgcaaca tataccagcc tcagcagcgt aaatgagagt aaaagcgtaa
     7681 gctgaaactg gcaggctccg ctaaaattac tacgcttaag agataaaatc tctttttaaa
     7741 caatgagtaa ttttcttata gggagtacat atgggtttct ggagaatcgt catcaccatc
     7801 attctgccgc cgctcggcgt gctgctcggt aaagggttcg gttgggcgtt cattattaat
     7861 attctgctga cactgcttgg gtatatcccc ggtttgattc acgcgttctg ggtgcaaacc
     7921 cgcgattaaa cctgtgctca cccccgcggc gttaacgcgc cgcgttcctc tgctacactt
     7981 tctgagtgtt tccgttaacg aatgaggtag ctatgggtct gttcaatttt gtgaaagatg
     8041 ccggagaaaa actctgggac gcggttacag gtcagcacga taaagacgat caggcgaaga
     8101 aggtgcagga gcatctgagc aaaaccggta tacctgatgc cgataaagtg aacattcaaa
     8161 ttgccgacgg caaagcgacg gtcactggtg acggcctgag tcaggaggcg aaggagaaaa
     8221 tccttgttgc ggttgggaat atttccggta ttgccagtgt cgatgatcag gtgaaaacgg
     8281 cgacaccagc cactgccagc cagttttata ccgttaagtc tggcgacact ctgagtgcca
     8341 tttccaaaca ggtctacggt aacgctaatc tgtacaataa aatcttcgaa gcgaataaac
     8401 cgatgctaaa aagcccggat aaaatttatc cggggcaagt gttgcgtatt ccggaagagt
     8461 agttaattgc cagccatcgc ctgctggata atgggaattg gcgtcagtaa atgctggcgc
     8521 attaactcac tggcgcgcgc ggtatctcgc gccagtaccg ccgcagtcag ggtctggtgc
     8581 tgatcgtgtt tatcctccag catttctacc gaaagcaccg ttcgccgcag ccagataaat
     8641 cgatagcgcg ccgccagatc aaacaaccgt tcacgcattt gcagcaaata gtgagaacca
     8701 cagcccgcca caattgctgt atgaaacgcc tgatgacgca gatcccactc atcaagcatt
     8761 ttctcgctgg cgtcacaggc ttccagctta ctgagcagat gcgcttttgc gagaacgtct
     8821 gcctcccatt catcgccacc gcgggcaatc gccagactga ccagcatcgc ttccatattg
     8881 gcgcgggcgt cgaaaatatc gagcagctcc tgctctgaca tagacgccac ccgataccct
     8941 ttttgattca ccaccgtgac cagccgttcc gccaccagtt gagaaagtgc ttcccgcaac
     9001 ggcccaacgc caagtgcata acgcgatgtc agcaaactca tccgtaattt ttcatccggt
     9061 tgaaaattac cgcgaataat atcgttcttc agccagcgat agccatccag agacgtaatg
     9121 gtcatggtgt gactcctgat cattatcagc gcgtattatg aacgggtgtt ttttgccaca
     9181 ataccagctt tttccagcga gccataattg gcacggtaca gataatccct atcgctaata
     9241 agccggtgga gatcacttct aactgttgcg ccggacggaa tagcatcact accaacacaa
     9301 aggtaataaa gccgatgacc agccaggtga gccagggata aagccacatg cgcaagcgaa
     9361 tttcgcttcc ttctgctcgc agaattttgc gcatccgcaa ctgtgaaacg gcgatgacta
     9421 aataaaccag cagggcgata gcgccggagc tgtctatcag gaatttaaac actttcgcag
     9481 gtgcgtaata gttcaccacc accgttaaaa atgccgctcc ggtggagagt aacaccgcca
     9541 catacggggt tttactgcgg ttgattttgc ccattagcgc gggcgcatca ccgcgacggc
     9601 ttaaggagta gagcatcctt gacgcggtat acagcgccga gttcagacag ctggttacgg
     9661 aaagtaatat cacgcagtcc atgattaatt tcgcatgggg aatattgagc aattccagaa
     9721 ccgagcgata agaaccaacg gctttcagcc ccggcatatt ccacggtatt aacgccacta
     9781 cgacaaaaat agagcacaaa tagaagatag aaatacgcca gataaccgag ttggtagcgc
     9841 ggacaatatg tttttccggc gtgtcggatt ccgcggcggc aatggtgaca atttctgcgc
     9901 ccataaacga gaacatggtg atcaacatcg cgcttaatac cgcaccgaag ccgttgggca
     9961 taaagccgcc gctatcccac aatcttgaga tcccgctcac ttcggcataa gggtaaaaac
    10021 cgctaattgc gactgcacca aggaaaataa aggccaggat agcgatgact ttgcacagcg
    10081 ccagccagaa ctcaaattcg ccgtagtttt taacgcttaa taaattactg ccagttaagg
    10141 cgagggtaat gacgagggaa aataaccaga tgggaatgcc tggaacccac gagtgcagga
    10201 tcatagcggc gatgttggct tccagcggga taaccagtac ccaaaaccac cagtacagcc
    10261 agccgatggt ataacccgcc cagcggccaa tggctttatc ggcataggtg gaaaacgaac
    10321 cggtatcggg cgtggcaact gccatttccg ccaacatccg cataatcata accaccagta
    10381 atccggcgaa cagataggcc agtaataccg ccgggcccgc ttcggcgatg gcgacgctgg
    10441 aaccgacaaa cagacttgcg ccgataacac cggcaataga caacatggtg acgtggcgtg
    10501 acttcagccc gccgcctaac tcatgtggtt gcgatgattg ccccatcctg aatcctctcg
    10561 aaagtgtgtc acttgggaga gcgccccgac atttcccgcg tcgggtcacg cgtttaattg
    10621 agacggtcaa caaaatctta aaaacgaaag cgggagagtt ttctccctct cccttaggga
    10681 gagggccggg gtgagggtgt agccgcgtga ttacccctca ccctaaccct ctccccagag
    10741 gagcgagggg acagtccgca gtcgttttta actttgtcga tcgcctcatc gttattattt
    10801 tttactgcat cgttatcatt cccttagcaa cgtttaactc ttacaaacgg acatagctac
    10861 tgcttcgcct cagcaaaaca ctggctgatg atctccagac cctgacggat ctgagcgtct
    10921 tcaatggtga gcggtacaag gatgcgcagc acgttgtaat acgggccgca ggagagaaga
    10981 atcaggccct tatcgcgggc gcgtgccacg atctcggcgg tgagttttgc gtccggtttg
    11041 ctgtgatcgc catcttcaaa cagctcgatg gcaatcatcg ctcccagccc gcgtacgtcg
    11101 ccgatctccg ggtgtttttc ggctatcgcc aacaatccgt cttttagctt ctgccccaga
    11161 tcgttggctt tttgcagcag attttcctgc tcaaacacct tcaacacttc cagcgccgcc
    11221 acgcaggcaa tcgggttacc ggcataggtg ccgcccagac cgcctggagc gacggcatcc
    11281 atcacttccg cgcgcccggt gacgcccgcc agcgggaagc cgcccgcgat cgatttcgca
    11341 aaggtagtga gatccggcgc aacgcccatc tgctccatcg caaacagcgt gccggtacgc
    11401 cccgcgccgc tctgcacttc atcggcaatc agcatgatcc cgtgctcgtc acacagcgcg
    11461 cgtaaacgct gcataaaggc tggcgacgcg gcgtagaaac cgccttcgcc ctgaaccggc
    11521 tcaatcacga tggcggcgat atcttccggc gcggcatcat ttttgaagat ccggtggatg
    11581 ctggcgatag cgtcatcctc gcttatgccg tgcagcgggc aaggataaag cgcgcgataa
    11641 acatgacccg gcatcagccc catgcccgca gagtacggat tcaccttgcc ggtcagcgcc
    11701 agcgtgtaat gcgtgcgccc gtgatacgcg ccgctaaaag cgatggtgcc gctacgtttg
    11761 gtggcggcgc gggcgatttt taccgcgttt tccaccgctt cggaaccggt cgtaaccagc
    11821 agcgttttct tggcgaaatc gcccggcacc ttctgattca taatctcgca cagctccaga
    11881 tacggctcgt aagccagcac ctggaagcag gtgtgcgaca gttttttcaa ctgcgcttcc
    11941 accgcggcca ccaccttcgg atgcaggtgc ccggtattga gcaccgcaat cccgcccgcg
    12001 aaatcaagat actcacggcc ttcaacgtcc cacacccggc agttttccgc gcggtcagcg
    12061 aaaatcgggt gaatttgccc aacgccacgg ggaatcgcct gactgcggcg ctgcattaac
    12121 tctttattgc tgttcattcg cattctccag ttaaagaccg atgcacatat atttgatttc
    12181 taagtaatct tcgatgccat acttcgaacc ttcacgaccc agacccgagg ctttgatgcc
    12241 gccgaacggg gccacttcat tggaaataat gccggtattg atgccgacga tgccgtactc
    12301 cagcgcttcg cccacgcgga agacgcggct taaatcacgg gcgtagaaat aggcggcaag
    12361 gccaaactcg gtgtcattgg cttgcgcaat cacatcggct tcatctttaa agcggaacag
    12421 cggggcgagg gggccgaacg tctcttcttt cgacactttg gcgttggccg gcacgtccac
    12481 cagaatggtc ggctggaaga agttgccgcc gcgttcatcc gctttaccgc cgcaaaccac
    12541 acgcgcacct ttctccagcg catcggcaat atgctcttcc acttttgcta ccgctttttc
    12601 atcgatcagc ggcccgatgg tgacgccttt atccagcccg tcgccgatgt gcagtttgct
    12661 caccgcctgc tgcaattttt cggcaaagcg gtcatacacg ccgtcctgca cgtataaacg
    12721 gttggcgcag acgcaggttt gcccggcgtt gcggaatttc gaggccagcg cgccttccac
    12781 ggctttatcg agatcggcat cgtcaaagac gataaacggc gcgttgccgc ccagctccag
    12841 cgacactttt ttgatgtctt tcgcgcactg ttccattaac tggcggccaa tttcggtcga
    12901 accggtaaac gacagtttgc gtaccatcgg gttgctggtc agttcattac cgaccgcgcc
    12961 tgccgaaccg gtgaccacgt taaacacgcc agccggaatg cccgcgcgaa tcgccagctc
    13021 cgccagcgcc agcgcagaga acggtgtctg actggcgggc ttcagcacca tggtgcagcc
    13081 tgccgccagc gccggaccgg ctttacgggt aatcatcgcc gccgggaagt tccacggcgt
    13141 gatagctgcg gtaacgccaa tcggctgctt gataacaatc aggcgtttat cggcctgatg
    13201 accgggaatg gtgtcgccat aaatgcgttt gccttcttcg gcaaaccact caataaaaga
    13261 ggcggcgtag ctgatttcac ctttcgcttc ggccagtggt ttgccctgtt cgagggtcat
    13321 caggcgcgct aaatcgtcct gatgctccat catcaaattg aaccagttgc gcagaatgtt
    13381 ggcgcgttct ttggcggtga gcgcacgcca ggcgggcagg gcgcggttgg cggcgtcgat
    13441 agcggcgcgg gtttcatcag cgcccatttt cggcacgcta cccagcttgt cgccgttcgc
    13501 cggattggtg acgtcgatga cttcaccatt gttggcgtcc agccattccc cgttaatcaa
    13561 cgcctgctgg cggaataagt tactgtcgtt aagtttcatt gttacgtcct gttttcaaag
    13621 gttattgatt aaatgcggcg tgtaaggcat ccacactacg tgccgctcgc agcgtgcgtc
    13681 cggggttact ctggcttgcc aacagcgttt gtaccttgct gacaatatgc gcaccaatag
    13741 gaattgctga tgtcgctgcc ggggagggcg cattgcaggt gtggatcgtg cgtggggtgg
    13801 tgacaaacag aaaatcgtca atcagcttgc cgtccggcga tactgcctgc gcccgcacac
    13861 cggcgggcca gggctggaga tcgcttaacg aaagccgggg acaatacttt tgcaccaggc
    13921 gcagatagcc gcttttgcac agcgagtttt tcatctcgcc cagtcctgag cgtagatggt
    13981 tttgcagcac ccggcgaatc cccgacgagc ccaaaatctc cagcgtgtcg ctaaatgaga
    14041 agtcgcgctt gcgatagcct tcgcgtttga aagccagcac cgcgtttggc ccgacggtga
    14101 cgctgccatc gatcattcgg gtgagatgca cgcccaaaaa tggcattgcc gggtcgggaa
    14161 tggggtaaat cagatggtta acaatctggt tatgctccgg cgcaaggcgg aaatactcgc
    14221 cacggaacgg gcagataata aaacccggtt cgaggccgag cattttcacc agccggtcag
    14281 ccatcagccc ggaacagcta atcagcgtcg atgcttcata ttcgccgccc tgacgggttc
    14341 ggacgaccac gccgttttta tgctcactga gggcgctgac ttcggcgtta taaataatct
    14401 caccgcctct ggcctggaag atttttgcca ttgccgctgt gacttcgcga tagctgacaa
    14461 tgccgctgga cggcacaaaa atgccgccga gcccggtgat attcggttcg cgctcgcgca
    14521 gttcctcggc gtttaaccac tcgcgctcga taccgttcgc cgccgtgcgt tcccacaacg
    14581 cgcgcatccg ttccatttcg agatcggacg tggcgaccag catcttgccg cagttgtcat
    14641 agcgaatgtc gttttgatcg caaaaggctt tggtggcgcg gtttcccgcc aggcaaaact
    14701 gcgccttcag gctgccgggc gtgtaataga ccccggcatg gatcacgccg ctgttgtggc
    14761 ccgtctggtg acaggccggg ccggactctt tttccagcaa cgcaatgcgg gcatccggat
    14821 aaacatcaat cagttgcatg gcggtcgaca tgccgatgat gccgccgcca ataatcacaa
    14881 aatcatacat ccgctcaatt cctttgcgct tactgatgcg tctggtagtg gtgagtggcg
    14941 taagcgaaat agccacgctg acgcatcagt tcacggcgca gatccgggtg cggagtaaag
    15001 cggtcgcgac cgtgcagcca gaacaggttg tttatcaaca ggaatttgcc aacgggaacg
    15061 ggtacagaaa gaatgccttt gctggtttca atggcatcgg aaagctcgct caaccacacg
    15121 ccttcttcaa aatcttttgg ctggacgaac tggtcgatat agcgcatcac cgggcgaccc
    15181 tgttgatcaa cgtcgaacac tggatggaaa acatctttgc tgacgttttt gctcggcggc
    15241 gcggcaaagc gcatcgggcg acgcgccagt ggatggcgga aatagtggtc cagatgttcc
    15301 cagtcatcga gatgcagcag caacgaattt ccaccctgca tgttttgctc atcgattttc
    15361 atcatcagca cgtaatcggt gatctcttcg acatacgtgc cgtcgttgtg cagttccatc
    15421 acgcggtgcg gctgacgcag atagctgtct gagttatcga catttttcac cacgaaacgc
    15481 gcgtaatact gaccgctcat cgcatcgaaa tttgagcgac caatcagatg cgctaccgcc
    15541 gttgccagct tcaccatctc atctgcctgc gccacatcat cgatacccac cgcattgatc
    15601 aacagcgcac cttcagcacg gtttagcagg gtcttcaaca gcaatggttg cagctgattc
    15661 gcacacagat cgtcaagaat tttgcctacc cgaaaacgca gaaacgattt gtactccagc
    15721 gcctgcacgg gccattcggc aacctgttcg agaaactgtt tggtcgtctg ttcggtgaag
    15781 gtgagttcca gcagacgcgg ggattgcgcc gacgggatga gagtgaatcc gctatagtcc
    15841 tggcctgaat cgacagcgtt attgtgtacg gcggtcagtg cattcatcag aagcgatcct
    15901 cttatgagat gtagggtgac atggcgatgc tcatttcgta gccataatct aaaaatatct
    15961 acatttctga aaaatgcgca caaaagcgac atattgtttt cttattgtga tcaaaagcaa
    16021 caaattagta acatttaacg tagggtatta aggcaaagtg agacaggaaa tgaataacta
    16081 aagcaacagg ttagggggag ttaacattag agaacagatt atctggtcga taaaaaacag
    16141 taaatggctt cactgcaaaa tgaccgttag taaactaaac atattatctg ccccgtt
//
LOCUS       DAZFKM010000109        16070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFKM010000109 DAZFKM010000000
VERSION     DAZFKM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..981)
                     /gene="ldtC"
                     /locus_tag="ACHKAY_003671"
     CDS             complement(19..981)
                     /gene="ldtC"
                     /locus_tag="ACHKAY_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO5702280.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1125..4571)
                     /gene="mfd"
                     /locus_tag="ACHKAY_003672"
     CDS             complement(1125..4571)
                     /gene="mfd"
                     /locus_tag="ACHKAY_003672"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO5702281.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(4699..5772)
                     /locus_tag="ACHKAY_003673"
     CDS             complement(4699..5772)
                     /locus_tag="ACHKAY_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO5702282.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6034..7233
                     /gene="lolC"
                     /locus_tag="ACHKAY_003674"
     CDS             6034..7233
                     /gene="lolC"
                     /locus_tag="ACHKAY_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO5702283.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7226..7927
                     /gene="lolD"
                     /locus_tag="ACHKAY_003675"
     CDS             7226..7927
                     /gene="lolD"
                     /locus_tag="ACHKAY_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO5702284.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            7927..9171
                     /gene="lolE"
                     /locus_tag="ACHKAY_003676"
     CDS             7927..9171
                     /gene="lolE"
                     /locus_tag="ACHKAY_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO5702285.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9200..10111
                     /gene="nagK"
                     /locus_tag="ACHKAY_003677"
     CDS             9200..10111
                     /gene="nagK"
                     /locus_tag="ACHKAY_003677"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO5702286.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10127..10948
                     /gene="cobB"
                     /locus_tag="ACHKAY_003678"
     CDS             10127..10948
                     /gene="cobB"
                     /locus_tag="ACHKAY_003678"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO5702287.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11087..11875)
                     /locus_tag="ACHKAY_003679"
     CDS             complement(11087..11875)
                     /locus_tag="ACHKAY_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO5702288.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(11872..12333)
                     /locus_tag="ACHKAY_003680"
     CDS             complement(11872..12333)
                     /locus_tag="ACHKAY_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO5702289.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            complement(12391..13437)
                     /gene="potD"
                     /locus_tag="ACHKAY_003681"
     CDS             complement(12391..13437)
                     /gene="potD"
                     /locus_tag="ACHKAY_003681"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO5702290.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(13434..14228)
                     /gene="potC"
                     /locus_tag="ACHKAY_003682"
     CDS             complement(13434..14228)
                     /gene="potC"
                     /locus_tag="ACHKAY_003682"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO5702291.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14395..15513)
                     /locus_tag="ACHKAY_003683"
     CDS             complement(14395..15513)
                     /locus_tag="ACHKAY_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO5702292.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            complement(15482..15751)
                     /locus_tag="ACHKAY_003684"
     CDS             complement(15482..15751)
                     /locus_tag="ACHKAY_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5702293.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            complement(15813..>16070)
                     /locus_tag="ACHKAY_003685"
     CDS             complement(15813..>16070)
                     /locus_tag="ACHKAY_003685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO5702294.1"
                     /translation="WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAID
                     FDARTAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
BASE COUNT         3817 a         4046 c         4145 g         4062 t
ORIGIN      
        1 atttttttta tccaggattt acagagtttg tgggctcact tgatgacggc ggacatccac
       61 cggcatcccg gaacggacat ccatcacatg ttgcatcact tcagcgtcag tttgtgctgc
      121 atctttaaat gattgcattg cgctattcag cgtaattggc agcagctgcg gatcgtcatc
      181 aatcttctct gacagcggct gatgtacttc aaccagacgc gcaccgtttg gttcggcaga
      241 gacttttatc ggagtgttga tgatattcac tttggtgcct ggggtgacct ggctaaagag
      301 tgttttgata tcgtcatccc gcagacgaat acagccagaa cttacccgca tgccaatgcc
      361 gaaatcggcg ttcgtaccgt gaagcaaata aacgccgcca taggccgcca gacgaatcgc
      421 atgatggccc attgggttat ccggtccagc cggcactacc gcaggcaact caattccctg
      481 tgctttataa cgggcgcgga tgtttgccgt tggcgtccag gttgggtttg cacgtttgtc
      541 tgaaacggtg gtcaccattg tcggtgtcag cgtgtcacca cctaactgac caatacctat
      601 tggatacacg gttaccgaat ttttacccgg cgggtagtaa tagagacgca gctccgcaat
      661 attgatcaca atgccttcgc gcggcgcatc tggaagtagg gtttgcaacg ggatcgttaa
      721 cacgctaccc gcgcgcggta cgtaaggatc aacgccaggg ttagcctgta acagagcgag
      781 aaagccgacg ttatattttt tggcgatggc ttccagagaa ccaccgtcat tttccaccac
      841 atgaaatttg ttttcgccaa ccagacgact gcccgctggc ggcagcggcc aggtgttggc
      901 ttttgccggt agcgccagcg ccacggcagc ggcgaacgta aaaaacgtta gccagcgaga
      961 aaaacgcgtt ttgatcatca ccaaaaatcc ataataaata taaggttatt gtaataaaca
     1021 gataagcctg aattatggat ggtgacagtg tcggatagtg cagggaagtg caaagaattt
     1081 gtaaatgttg cagatggggg cgcagaaacg cccccgattt accattaagc gatcgcgttc
     1141 tcttccagtt cacgcataaa ctggcgtacc cattcgatac gcgttttccg ctcactcaaa
     1201 tcctgaataa atttcaggcg cgtcggacca tcaaggcggt aatgctgcgg ctgtttttgc
     1261 agcaaaccaa tcaaccaggc cggattaacg tgattcttct cggcaaattc gatcaccccg
     1321 cctttctcat taccttccag cttcctgatc cccagtttct gcgcttgctg gcgcagacgg
     1381 gcaacatcca gcagggtacg cgccggatcc ggcagcaggc cgaagcgatc gataagctcg
     1441 actttgatct cttccagttc gttttccgtt ttggcgctgg caatacgttt atagaacgac
     1501 aaacgcgtat tcacgtcagg aatgaaatca tctggcaata gcgacggcat ccgcagctcg
     1561 acttctgttt gctggctggt gagatcttcc agcgacggct cgcgtccggc tttcagtgca
     1621 tcgacggcgt tttccagcaa ctccatatac agcgagaaac cgatggtttc cattgagccg
     1681 ctttgttctt cgccaagcag ttcacccgcg ccgcgaatct ccagatcgtg cgttgccagc
     1741 gcaaaacctg ccccgagatc ttccagcgag gcaatcgctt caagacgttt ttgtgcatcg
     1801 gtagtcatcg cttttggatg cggcgtcagc aaccatgcat atgcctgatg atgtgaacgt
     1861 ccgacgcgac cgcgtaactg gtgcagctgc gccagaccga agtgatccgc gcgttcaatg
     1921 ataatggtgt tggctgtcgg gatgtcgatc ccggtttcga taatggttgt acaaaccagc
     1981 acgttgaaac gttgatgatg gaaatcattc atcacccgtt ccagttcgcg ctcgcgcatc
     2041 tgcccgtgac cgatggcgat ccgcgcttct ggcaccagtt ctgccagccg ttcggcggct
     2101 ttctgaatgt tttccacatc attgtagaga taataaacct gtcctccgcg caaaatttca
     2161 cgcaggatcg cctcccggac caccaggctg tcatactcac ggacaaaggt tttaactgcc
     2221 agacgacggg cgggcggcgt ggcgataatc gacaggtcac gcattccgct cattgccata
     2281 ttcagcgtac gtgggatcgg cgttgcagta agcgtcagaa tatccacgtt cgcgcgcatc
     2341 gctttaatac gctctttatg acgcaccccg aagcggtgtt cttcatcgac aatcagcagg
     2401 cctaaatctt taaacttgac gtcactttgc agcagtttgt gcgtaccgat cagaatatcg
     2461 attttccctt ccgccacttc cgcaaggatt tgcgtctgct ctttggcgct gcggaaacgg
     2521 gagatcattt cgatacgtac cggccagttg gcgaaacggt cgcggaagtt gtcgtaatgc
     2581 tgctgcgcga gaagggtggt aggcaccaac accgccacct gcttgtggtt atctactgcc
     2641 aggaaagcgg cgcgcatcgc cacttctgtt ttaccaaagc caacatcgcc gcacaccaga
     2701 cgatccattg ccagcggctg acacatgtcg ctaagtaccg cattaatggc ctgcgcctga
     2761 tccggcgtgg tttcaaacgg gaagctgtcg cagaacaact gatactgctc acgatcgtgt
     2821 ttaaacgcga agccctcttt ggcggcgcgt tgcgcgtaga tatccagcaa ttccgccgcc
     2881 acatcacgca ctttttccgc cgctttctgc cgcgcgcgtg accacgcatc gccgccaagt
     2941 ttatgcagcg gggcgttttc ttccgcgcca cctgcgtaac ggctaatcag atgcagtgac
     3001 gacaccggaa catacagttt ggcgtcgttg gcataggtga gcatcaaata ctcgccagta
     3061 atgccgcccg cttcgagcgt ggtcattccg gcatagcgcc cgacaccgtg ctccagatgg
     3121 accaccggct gaccaatatg cagctccgca aggttacgga tcagtgtatc ggggttgatg
     3181 gtgcggcgag aatcctgacg acggcgggca acacgttcac cgagcagatc gctttcgcag
     3241 atcagcgcca gattacgcac cttatcgaca aaaccatgtt cggcagcgcc aatcatcaga
     3301 taacgcccac ggtcgctggc ttcatcaaga cgcataatgc gttgcggagc aattttaatt
     3361 cgtgcgagca gttcacccag cgcttcacgg cgaccttcac tttctaccga gaacaccacc
     3421 ggaccgtcga aagtctcgag gaacttacgc agcgcatcca gcggcgcttt ttgttgtgcc
     3481 tgaacggcca ggtctggcag tttctggaaa cctaaattgg cattcgcggc ttttgtcggt
     3541 aaatgttcag tttttagctg cacccggggc cagtttttca gctctgagaa gagctcgtcc
     3601 acccgcagcc agagcgattg tggtggcaac agcgggcgca tcggatcgac gccgcgattc
     3661 tcaaaacgcg ccagcgtgtc agcctggaaa cgttcggcac tggtttccag atcgccagta
     3721 ttcacgagca aggtattggc agggaaataa ctgaacagcg gcggcagtgg ttcgctgaag
     3781 aacaatggct gccagtactc gatcccggca ggtaatgtgc ctttactcac ttgctggtaa
     3841 atatgttccg gatcgcgctt cacttcgaag gtatcgcgcc actggctgcg gaacagttca
     3901 attgccgctt tatcggtcgg aaattcgtgc gcgggcagca gattgatcgc ttctacttcc
     3961 tccagcgtgc gctggctgtc gacgtcaaac acccgcaggc tgtcgatttc atcatcaaag
     4021 aaatcaagac gataaggcag ctcactcccc atcgggaaga gatccagcaa cgcgccgcgc
     4081 gtggcgtatt cgccgtgctc catcacctgg tcaacatggc gataaccggc gctgtccagt
     4141 tgggttcgta atgcatctcg tgacaggcgc tgaccttttt tcatcaccag cgcatgaccg
     4201 tggagaaaac tgtgtgggca aacgcgctgc ataagcgtat tcaccggaac aatcagtacg
     4261 ccacgctgca tcgtcggtag ctggtaaagg gtggaaaggc gcgaggagat aatgtcctga
     4321 tgaggcgaaa aactgtcgta gggaagagtt tcccagtccg ccagattcat caccatctga
     4381 tcggtgaact ggctgatttc atcatgcaaa cgcagagcat tttgcatatc tggtgcaatg
     4441 agtaccaccg gaccggcgtg acgttcggca atttccgcta ccagcgttgc acaggctgcg
     4501 ccggttaact cgcccagcag acgctgctca cccgctttga cgggcagcgt ataacgatat
     4561 tgttcaggca taacggttgt cagattctcg ttaggatatg cctcaacata tgggggcata
     4621 tctctgataa gcaatgctgt tcattatccg taatcgacat ggtttagcaa attgaatcgc
     4681 cccggctggg gcgatggttt aacgagagta actgacggca gcgggcagtg aaggcgggga
     4741 gaacaacaga tcgctgaatg cacgctgtga aagttttctt accagcaagc ccgcaacagt
     4801 acagatgaac aaactgacaa agggataaac cagcaggagt gtcagttcga cttgcggcga
     4861 ccagcgtgct gcgttcattt gcgcaagcag agttaagctg aatatttcaa ccagaatgcg
     4921 atgggtggtg tagatagcaa tggtgttgga gccaatcaca ttcagcaggc tggtggagcg
     4981 cataccgaat cgttgctcgt attgataaaa cagcttcatg atcaccacaa tcgataccag
     5041 cgacaacagc agcgagatag taaacaacca ggcaccgacc gccagaacgg taagcaaaga
     5101 agccatcagc agatggcggc gcaacggcac ctctttaaca caggtcatta ttgttgcgcc
     5161 aaaccatgcg ccaaggctgt aataaagcaa attgcggatc acactgttca ttccccacca
     5221 cggcgtggga acgaaattaa ccgccacact cagcagtaca aacaaggcga atagtggcag
     5281 cgccagacgg ctaaaaattt tacataccac gaaatagaca attaacgcat acagatacca
     5341 caagctggtg ctggcggtga tcatcccgtg caggaactca ccggtggaat cggcataagc
     5401 ggcattggag gcattgctta aatcgcgctc aggtgccagc cactggttca gcgcacttaa
     5461 cgccagccac tgcaccacgc cccaaagcgc cagcacccag aagatgttcc agatccgttt
     5521 atcgagacaa tttccccacg gtacgctgtc gatatagcgg cgaatcaaat agccggatat
     5581 aaagaaaaaa accggcatac gaaagggggc aaggtaaaga ttgaaataga tccagcattt
     5641 gctcaggact tccgataacg gatgctgaaa agtggtcaga tgcggataaa aggtaatgac
     5701 cgagtgataa atcaccacca gacaaataca taaccctttg atctggttaa tccatagctc
     5761 tttttgtttc atcaggacaa cacgctcttt tttaatttac atgcggttga tcctgcgggt
     5821 tatgacccga ggtgtaatgg ggatctgtct gtattggatg atttttcaga ttaagatcag
     5881 gcggcaagat tgatgataaa acatggcaat ttagccgatt gatttacggg ggcttttcag
     5941 attagccctg acgatcactt acagttcaga cgtttaccca tcttgctttc gcttatatac
     6001 tcgtgtcttt gctacagcaa ccagacggat ttcatgtacc aacctgtcgc tctatttatt
     6061 ggcctgcgtt acatgcgtgg gcgtgcagcg gatcgcttcg gtcgtttcgt ctcctggctt
     6121 tctaccatcg gcattaccct cggggtgatg gcgctggtca cagtattgtc agtgatgaac
     6181 ggctttgagc gcgagctgca aaacaacatc cttggcctga tgccacaggc aattctctct
     6241 tctgagcatg gctctcttaa cccgcagcaa ctcccggaaa cggcagtcaa actggacggc
     6301 gttaatcgcg tcgcacctat tactaccggt gatgtggtac tgcaaagcgc gcgcagcgtg
     6361 gcggtcgggg tgatgctcgg tatcgacccg gcgcaaaaag atccacttac accgtatctg
     6421 gtcaatgtga aacaaactga cctcgagccg gggaaatata atgtcatcct cggcgaacaa
     6481 cttgcctcac agctaggcgt taatcgcggt gatcaaatcc gcgtgatggt accatctgcc
     6541 agccagttca cgccgatggg gcgtattcca agccagcgcc tgttcaatgt gattggcact
     6601 ttcgccgcca acagtgaagt cgatggctat gaaatgctgg tgaatattga ggatgcctcg
     6661 cgtctgatgc gttatccggc aggcaatatt accggctggc gtttgtggct ggatgagccg
     6721 ctgaaagttg actctttaag tcagcaaaaa ctgcctgaag gcagcaaatg gcaggactgg
     6781 cgtgaccgta aaggcgagct gttccaggcc gtacgcatgg aaaaaaatat gatgggcttg
     6841 ctgctgagcc tgattgtcgc cgttgcggcg tttaacatta ttacctcact agggctgatg
     6901 gtaatggaga agcagggcga agtcgcgatc ctgcaaacgc aaggcttaac tccgcgacaa
     6961 atcatgatgg tctttatggt gcaaggggcc agcgccggga ttatcggtgc gatcctcggt
     7021 gcggcgcttg gcgcactgct tgccagccag ttaaataatc tgatgccgat aatcggcgtc
     7081 ctgcttgatg gcgcggcgct gccggtcgct atcgaacctt tacaggtcat tgttattgcg
     7141 ctggtggcga tggctatcgc gctgctgtct acgctttacc cttcatggcg cgctgccgcc
     7201 actcaacccg ctgaggcttt acgttatgaa taagatcctg ttgcaatgcg acaacctgtg
     7261 caaacgctat caggaaggca gtgtgcaaac cgatgtgctg cacaatgtca gtttcagcgt
     7321 cggcgaaggt gaaatgatgg cgatcgtcgg tagctctggt tccggtaaaa gcaccttgct
     7381 gcacttgttg ggcgggctgg atacgccaac ctccggcgat gtgatcttta acggtcaacc
     7441 gatgagcaaa ctgtcttcgg cggcgaaagc agagctgcgc aaccagaagc tgggctttat
     7501 ttatcagttt caccacctgc tgccggattt taccgccctg gaaaacgtgg ctatgccgct
     7561 gctgattggc aagaaaaagc ccgctgaaat caacagccgt gcacttgaga tgttaaaagc
     7621 ggtggggctg gatcatcgtg cgaatcaccg cccatctgaa ctttctggcg gcgaacgcca
     7681 gcgtgtggcg attgcccgtg cgctggtgaa taacccgcgc ctggtactgg cggatgaacc
     7741 taccggtaac ctcgatgcgc gtaacgccga cagcatcttc cagttgcttg gggaattgaa
     7801 tcgcttgcag ggcaccgcct tcctggtggt tactcacgac ctgcaactgg cgaaacgtat
     7861 gagccgccag ttagaaatgc gtgatggtcg tctgacggcg gaactgagcc tgatgggggc
     7921 ggagtaatgg cgatgccttt atcgttatta attggcctgc gttttagtcg cggacggcgg
     7981 cgcggcggca tggtgtcgct gatctccgtt atttctacca ttggcattgc cctcggcgtg
     8041 gcggtattga tcgtcggctt aagcgcgatg aacggctttg aacgcgaact gaacaaccgt
     8101 attctggcgg tggtgccgca tggtgaaatc gaggcggtgg atcagccgtg gactaactgg
     8161 caggaagcac tggatcacgt gcaaaaagtg ccgggtattg ccgctgccgc gccgtatatc
     8221 aatttcaccg ggctggtgga aagtggcgcg aatcttcgcg caatccaggt gaagggcgtt
     8281 aacccgcaac aggaacagcg tctgagcgca ttaccctcgt ttgtccaggg cgatgcgtgg
     8341 cgcaatttta aagcgggcga acagcaaatt atcatcggca aaggcgtggc ggatgcgctg
     8401 aaagtgaagc agggggattg ggtgtcgatt atgatcccca actcgaatcc cgagcataaa
     8461 ctgatgcagc caaaacgtgt gcgtttgcac gttgccggta ttttgcagtt gagtggtcaa
     8521 ctcgatcaca gttttgccat gatcccgctg gcggatgccc aacaatatct tgatatgggt
     8581 tccagcgtgt caggtattgc ccttaaaatg acggatgttt tcaacgccaa taagctggta
     8641 cgcgatgcgg gtgaagtgac caacagctat gtttatatta aaagttggat tggtacttac
     8701 ggctatatgt atcgcgatat ccagatgatc cgcgccatta tgtatctggc gatggtactg
     8761 gtgattggcg tggcctgttt caacatcgtc tccaccttag tgatggcggt gaaagacaag
     8821 agtggcgata tcgcagtatt aagaacgctg ggggcgaaag atggtttaat tcgcgccatc
     8881 tttgtctggt atggattgct ggcagggctg ttcggcagcc tgtgtggggt gattatcggt
     8941 gtggtggttt cactgcaact taccccgatt attgagtgga ttgaaaagtt gatcggtcat
     9001 cagttcctct ccagcgatat ctattttatt gatttcctgc catcggaatt gcactggctg
     9061 gacgtcttct acgtactggt cacagcattg ttgctgagtc ttttggcaag ttggtatccg
     9121 gcgcggcgcg ccagtaatat tgaccctgcg cgagtcctta gcggccagta aaggcagtac
     9181 attaaaacaa ggagcggcaa tgtattacgg gtttgatatt ggtggaacaa aaattgcgct
     9241 tggcgtgttt gatagcggtc ggcagttgca gtgggaaaag cgggtgccga caccgcgtga
     9301 cagctatgac gcatttttag atgcagtgtg tgagctggta gctgaagctg accaacgttt
     9361 tggctgtaaa ggttctgtcg gcatcggtat tccgggaatg ccggaaacag aagatggtac
     9421 gctgtatgcc gccaatgtcc ccgctgccag cggtaaaccg ctgcgtgccg acctgagcgc
     9481 acgtcttgat cgcgatgtac gccttgataa cgatgccaac tgttttgccc tttcagaagc
     9541 ctgggatgat gaatttactc aatatccact ggtgatgggg ttgattctcg gcaccggcgt
     9601 tggcggcggg ctgattttca acggcaaacc gattaccggt aaaagctaca ttaccggcga
     9661 gtttggccat atgcgtctgc cggttgatgc gttaaccatg atggggttgg atttcccgtt
     9721 acgccgctgc ggctgtggtc agcatggctg cattgaaaat tatctgtctg gtcgcggttt
     9781 tgcgtggctg tatcaacact attatcatca accgttgcag gctcccgaaa ttattgcgct
     9841 ttatgatcaa ggcgatgagc aggcaagggc gcacgttgag cgttatctgg atttattagc
     9901 ggtttgtctg ggaaatatcc tgaccattgt tgaccctgac ctggtcgtca ttggtggtgg
     9961 cttatcgaat ttcccggcaa tcacaacgca actggcggac aggctgcctc gtcatctctt
    10021 acctgtagct cgtgttccgc gcattgaacg cgcgcgccac ggtgatgcgg gaggaatgcg
    10081 tggtgcggcc ttcctacatc taaccgatta aacaacagag gttgctatgc tgtcgcgtcg
    10141 gggtcatcgg ttaagtcgtt ttcgtaaaaa taaacgccgc ctgcgcgagc gtttgcgtca
    10201 gcgtattttt ttcagagata aagtggtgcc ggaagcaatg gaaaaaccaa gagtactcgt
    10261 actgacaggg gcagggattt ctgcggaatc aggtattcgt acctttcgcg ccgcagatgg
    10321 cctgtgggaa gaacatcggg ttgaagatgt ggcaacgccg gaaggtttcg atcgcgatcc
    10381 tgaactggtg caagcgtttt ataacgcccg tcgtcgacag ctgcagcagc cagaaattca
    10441 gcctaacgcc gcgcatcttg cgctggctaa actgcaagac gccctcggcg atcgcttttt
    10501 gctggtaacg cagaatatag acaacctgca tgaacgcgca ggtaatacca atgtcattca
    10561 tatgcatggg gaattgctga aagttcgttg ttcgcaaagt ggtcaggttc tcgactggac
    10621 cggagacgtt acgccagaag ataaatgcca ttgttgccag ttcccggcac ccttgcgccc
    10681 gcacgtcgtt tggtttggcg aaatgccact cggcatggat gaaatttata tggcgttgtc
    10741 gatggctgat attttcattg ccattggtac ttccgggcat gtttatccgg cggctgggtt
    10801 tgttcacgaa gcgaaactgc atggcgcgca caccgtggaa ctgaatctcg aaccaagtca
    10861 ggtcggtaat gaatttgccg agaaatatta cggcccggca agccaggtgg tgcctgagtt
    10921 tgttgaaaag ttgctgaagg gattataaag caggaagcat tgcctgatgc gctacgctta
    10981 tcaggcctac aaggggacgc aattaattga atttgcgggt tttgtgggcc ggataaggcg
    11041 ttcgcgcccc atccggcata atacacgcgc acttttccgg cctcttttat cgtttactcg
    11101 tcaataacaa tgcggcaata aacgtacaga caccaattag ccctgcccag agtggcgata
    11161 ttctggtaat cgcaaagcta ttatcgatat acaaacataa agcctgataa cccatattgg
    11221 caatagtcat cgccactgca aattttaacg cataaataaa acgagtcgtg tagcaaaaga
    11281 tccaggaaaa taagataaac gtcgcagccc aggcgataaa acctaatgtg ttggtgctgc
    11341 cggggggagc attttcatgc tgttgtcctg gatgcggata atacgtcagt ggcggcgtcg
    11401 gttgttttaa tatctgtgct tgtagaacag ccaatgcatt attgattccc tgataccaga
    11461 gatctgcatt taaataagcc agcgtgtgat tttctatcag aaggccgaga tgtttatcgt
    11521 ccagaccact ttcaacctgg ccgttggtga tcataattaa ccgtggttcg cgggtagtaa
    11581 atagtaacag aacacttcct tttacattac tcaatgaaga tgacgcatgc gccagcatcg
    11641 ctttaccatt ttgtctgaga ctggcttcat tattaatgtc gggtaaaatc acaataaacg
    11701 gtgcaattcc cgtttgttgg cgaaaaagct cggctttacg tgcgatctct tcagcatccg
    11761 gtacagtacc ggtaacatcc gtaaaaagtg tcttcattat tggaatcaat ggtttactgg
    11821 ttgctaccag aggcaggagt atcagtaaac tgagcagtat aatgaatttt ttcatgggca
    11881 ggacttccgt ttcctgcgag attttttggt ggaggggagc gtgattatca gaccgacact
    11941 accgaaaata atgcctgcta tgccaacaaa gccccattta aaaaaattaa cccagggatt
    12001 gtttatttcg gcttgttctg gatgccctgg cggataatag acattgactc tgtcaccttc
    12061 gctatcttca taaaaatcac taccgatatt cgaattgaaa attagcgtgt aatctggcgt
    12121 agggcgaaag gcaaccactg gataccagga accattatca tcgacgtcgt ttgagtgatt
    12181 gttgtaccaa acggcatcaa caatgattcc ttctgtatga accgcgcttt tggtaaaagt
    12241 aaaatggtaa taaaagatga ttgcagaaat aacgagcaat atcagaccga taccgagaaa
    12301 agcgcgggat aacactttac tgtcctgact cattagccac atccttgcta aataaaaaac
    12361 gggcggtaat gccaccgccc gcttgctgaa ttaacgtcct gctttcagct tctgataata
    12421 ctcttcataa atgctgctgg ctgcgccaac gtcattctgc cattcaccat ttttaatggt
    12481 ttcagcatcc gggtagagcg ttttatcgtt cgccacttcc gggcttaaca gcttacgcgc
    12541 cgcaaggttt ggcgttggat aaccgatagt ttcagcaacc tgttttgcca catccgggcg
    12601 cagcaggaag ttgatcaatt tcagcgcgcc ttctttgttt ttggcatttg ccgggatcgc
    12661 caggctgtcc atccagaaaa tgccgccttc tttcggccac accacgtcaa ttggcgtacc
    12721 cgcctgccgt gcaacgaaag cagaaccgtt ccagatcatg ccgaggttaa cttcgccttc
    12781 catgtacggg ttagctgggt tatcggagtt aaacgctgcg acgtttggca tcagtttttt
    12841 cagctcgtta tatgcagctt caatctcttt cggatcggtg gtgttaccgg agtagcccag
    12901 cttacgcagc gccatctgga acacttcacg ggcatcgtcg gtcaacagca ggctgccttt
    12961 gtactcaggc ttccacagat cggcccagct ggtgacagat ttcggatcca ccgcatcacc
    13021 gttaacacca atcgccgtcg caccccagat atacggaatg gagtaatcgt tattcgggtc
    13081 aaacggcttg ttgagcatgt ctggatcgag attgctgaag ttcgttaact tcgacttgtc
    13141 gatcttctgg atcatccctt ctttacgcat tttatcgacg taataggttg aaggaaccac
    13201 caggtcatag gcaccgtctt tgtacgtttt cagcttggcg tacatggttt cgttcgactc
    13261 gtaagtcgaa tagataacct taataccggt ttctttggtg aactgttcaa gcagtcctgg
    13321 cggcacgtac tcggtccagt tgtagaaata cagcgtgttg ttgtcatcgg cgtgagcggc
    13381 gctcatgccc agtgccagag cacccgccgc gagcaggtgg cgtgaccatt ttttcattta
    13441 acgtcccctg tgttaccttt cgttttatca cgagcaataa gctggctggc aataaccatc
    13501 accagcgaca gcaccagtaa tatggttgcc agcgcgttaa cttccggcga tacgccgact
    13561 ttgaccatcg aataaatttt taacggcaga atttcataac ttggtccggt gacgaacgaa
    13621 gaaactacca catcgtccat cgacagggta aagcttaaca cccagcccgc cgccaccgca
    13681 ggcattgcca gtggcagaat gatttttcgc agaatggtaa attcactggc accgagatct
    13741 ttcgccgctt ccagcatccg cacgtcaaaa cctttcaggc gcgaatacac cgtcaccacc
    13801 acaaacggca ggcagaaggt gatatgcgag aacagcagcg accagaagcc aagctgaata
    13861 cccagcagca taaacagcac cagcagagaa atcgccatca cgatatctgg tgacatcatc
    13921 accacaaaca gcattccgct aacgaacggc ttaccacgaa agcgataacg gtacagcgca
    13981 actgccgtca gtgaaccgat aagcgtagca aatgtcgccg aaaacaccgc cattgttagt
    14041 gaatgctgcg ctgcctgtaa caggctgtcg ttgttcatca gcaggctata ccatttggtg
    14101 gtaaaaccct gccagttgat gccaaagcgc gagctgttaa aggagttcac aatcaaaata
    14161 atgattggga tatacaggta cgcgtagata gcggtcataa aaccgccgcg aagcagtcga
    14221 ccgatcattc gagttccacc tttttattca taaggcggga cactttcagg attttgaatt
    14281 tattcaattt tttcctttct ccaaaatctc tccaaaacgt ctccccaaaa ctaggccacg
    14341 cattttacaa cacttttttc ctgagatggt gacggatatt ccatatggtg tttatcaaat
    14401 aaccaactta acccattcct gacctcgagt atcgttatag cgatcggtgg ttgcctggac
    14461 tttatgtcct agtaatgttt ttgtatcaat accctgtgca cggtacagcc gttctgatag
    14521 agagcgttgt tcatgaaatg ttggcggagt ttttcctgct ggtggaatta tcccagccag
    14581 atcccgtgct ttggcaaagt agtcgctcag gttgtcttta ctcatcggct tcggttgttt
    14641 ctggtgccga ctatggatta gatatggact taatattctg tctcggcacc catcaataac
    14701 ttcttttaac gttatcccaa tggcatcaca gcgtagtgta agcggtaacg ccagacgcat
    14761 tccggttttt ccctgggtga tatgcaagtg ttcgttccac acatctgaaa aacgcatgtg
    14821 gcaaatgtca tcacggcgct gaccagtaac aatcgcaaga agcattgcgt tacggataaa
    14881 gtgtttttca ggcgttgcat tgtaaatttt ttgccagtct tccatggtga gcctggctct
    14941 ggttacttta gggatcggtt tacgggtagc ctccggagga ttccatccag gaggaacttc
    15001 ccctgcatgc tgtgcttctt tataaatatc aacccataat ccacgattta ctctcgctgt
    15061 gctgaccatg tctttatcca gccactcatc cagtattaat gcaaagtctc ttacttccag
    15121 ttctttcaat gggtggtttc ccagacggga aaccaggtat gcagccattc gggttttttc
    15181 tttgtgagtt gtagctgcaa tatctccatt tttcagtcgc gtgtcctgta ttttcagata
    15241 tcgatcaacc catgccttta atctgatacc ccgacgtttt gttgctgacg gactttcatc
    15301 aattttgcgc atgaaatatt cagcttctgc tgcagctatt cgctgattgg ctgtggaagc
    15361 gattttttct gccttacctt tgtctgttcc gagtccgtga aattttccag tcacaggatt
    15421 tttatactgg tagtaaactc tgccagttct gcgatcaaac ttttcgtaaa gaccggctac
    15481 gtcagtgctg ttttttcgtg gcctcggtga catgagttaa aatctccttc agtgcatcat
    15541 catcgccagt atgaatttcc ggcgcaattc ccgtttcacc aggcccaaca aatactgctc
    15601 ggcgatctat cagccaacgc ccacgaattt tttgtggtct tggaacgatg tatcctagtt
    15661 ttccgtattt caccagggta gtgtttgtta ttgggagact gaaccgtttt ggtttccact
    15721 cgtcgagcgt tatcaggtac tgttcgctca tggctatcac tccggaacgc gccagttgca
    15781 gaatatcaac gacaactggc gacggttgaa cattaaaaat cagcctgatt cgggatcagt
    15841 ttttgccaga tagctgaaac gtattttgcc tggtgacgag cgtcatcaag tgcattatgg
    15901 cgctcacctt cgaatggaat agccgttctg gcatcgaagt ctatggcttt ccccagctca
    15961 acgattgtgc gtacatcgcg atcgttgtag taacgccacg ggcaggggat cccctgccgt
    16021 tcgtatgaac ggcgcaaaat cgtgttgtcg aagttggctc catttcccca
//
LOCUS       DAZFKM010000110        16016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFKM010000110 DAZFKM010000000
VERSION     DAZFKM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            558..1382
                     /locus_tag="ACHKAY_003686"
     CDS             558..1382
                     /locus_tag="ACHKAY_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFO5702295.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
     gene            complement(1431..2003)
                     /locus_tag="ACHKAY_003687"
     CDS             complement(1431..2003)
                     /locus_tag="ACHKAY_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702296.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIYNINISWASKPKSNDR
                     ETNEYVYDMATFEGISYLGARSPLTQIAEQIKGIREDWKPIAQGAKKVKADVYTSSDR
                     NEERTYLQEQHDLAIKRRDEKREKRLESGE"
     gene            2104..3274
                     /locus_tag="ACHKAY_003688"
                     /pseudo
     CDS             2104..3274
                     /locus_tag="ACHKAY_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3357..3614
                     /locus_tag="ACHKAY_003689"
     CDS             3357..3614
                     /locus_tag="ACHKAY_003689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702297.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(4172..4939)
                     /gene="fecE"
                     /locus_tag="ACHKAY_003690"
     CDS             complement(4172..4939)
                     /gene="fecE"
                     /locus_tag="ACHKAY_003690"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO5702298.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4940..5896)
                     /gene="fecD"
                     /locus_tag="ACHKAY_003691"
     CDS             complement(4940..5896)
                     /gene="fecD"
                     /locus_tag="ACHKAY_003691"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO5702299.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5893..6891)
                     /gene="fecC"
                     /locus_tag="ACHKAY_003692"
     CDS             complement(5893..6891)
                     /gene="fecC"
                     /locus_tag="ACHKAY_003692"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO5702300.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLAMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6888..7790)
                     /gene="fecB"
                     /locus_tag="ACHKAY_003693"
     CDS             complement(6888..7790)
                     /gene="fecB"
                     /locus_tag="ACHKAY_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO5702301.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7835..10159)
                     /gene="fecA"
                     /locus_tag="ACHKAY_003694"
     CDS             complement(7835..10159)
                     /gene="fecA"
                     /locus_tag="ACHKAY_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO5702302.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(10246..11199)
                     /gene="fecR"
                     /locus_tag="ACHKAY_003695"
     CDS             complement(10246..11199)
                     /gene="fecR"
                     /locus_tag="ACHKAY_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO5702303.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(11196..11717)
                     /gene="fecI"
                     /locus_tag="ACHKAY_003696"
     CDS             complement(11196..11717)
                     /gene="fecI"
                     /locus_tag="ACHKAY_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO5702304.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11854..11922
                     /gene="ykiE"
                     /locus_tag="ACHKAY_003697"
     CDS             11854..11922
                     /gene="ykiE"
                     /locus_tag="ACHKAY_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO5702305.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            12009..12706
                     /locus_tag="ACHKAY_003698"
                     /pseudo
     CDS             12009..12706
                     /locus_tag="ACHKAY_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(12717..12788)
                     /locus_tag="ACHKAY_003699"
                     /pseudo
     CDS             complement(12717..12788)
                     /locus_tag="ACHKAY_003699"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12851..12997
                     /locus_tag="ACHKAY_003700"
                     /pseudo
     CDS             12851..12997
                     /locus_tag="ACHKAY_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13024..13128
                     /locus_tag="ACHKAY_003701"
                     /pseudo
     CDS             13024..13128
                     /locus_tag="ACHKAY_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13432..13725
                     /locus_tag="ACHKAY_003702"
     CDS             13432..13725
                     /locus_tag="ACHKAY_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO5702306.1"
                     /translation="MVKLLTEPWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVI
                     EEAVNALFCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(14045..14176)
                     /locus_tag="ACHKAY_003703"
                     /pseudo
     CDS             complement(14045..14176)
                     /locus_tag="ACHKAY_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14458..15816
                     /locus_tag="ACHKAY_003704"
     CDS             14458..15816
                     /locus_tag="ACHKAY_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO5702307.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT         3929 a         4047 c         4212 g         3828 t
ORIGIN      
        1 tgtgcgagtc cggccttcgc accaagttac taaattcctt acaatacagt tagttagacg
       61 attaaatctg tctcttttgc ctgtaagaaa tgctctgtta tgtcccttga aaaagccgct
      121 gtggttgata gtggtaattg ggatgttaac tcatagcggc gtgtcgttcc aagcgtcaaa
      181 cacgtactga ctcaacttga aagctatgac gtagccaccg tctctaaaca atcccaaaac
      241 ttttagtgca gcgcatttta gcccgatgcc cccattctgt cgccgtcgcg agacgccgca
      301 ggggataaac aggtttatgg tggtcacggt acttgcagac catatgcaag ctctgccagc
      361 ggttcggcct cgctcccctt gggtacatgg tcatcctgat aagcctcact aactgacata
      421 accctgatga agtcgcaaga ggctccgctt gttgtcttac caccaacggg gctttatgga
      481 ttctattgct taatatatga ttgtatcaaa cttagactgc ataatttaaa cgaacataac
      541 aatcaggcag gggaagaatg ataaagccat tttctgtgag gtatggtcat gttgacgtta
      601 gagagcatgt ccagctgaat gatcttaatt ctgatacacg catggcattg tggaattgct
      661 tatatctttt cctttggact aacaatcgtc aaactgctac agcaacaaaa tgtgcgcagt
      721 ctgtgtggat atattatcta aatcaacctg cggataatat tcctcgttat gaaagtggat
      781 acaaaagtga taagacgttg ctgactgcta tccgagatta tatatatggg gaagcatggt
      841 atttggtata tgatttaatt gagttcatca ttgagagaac taattcatat attaatctat
      901 cgaagcatct caattccatc tttaaaaagc atggcgttgg ttataccata ataaatggtt
      961 gtattactcc tatttcaaat gataacgaaa ttgaaagtgt tcagaacgca gttgataatg
     1021 gcactgattc atcgcgtagc catttcgaaa gggcgttaca actaatgacg gatagagagc
     1081 agccagacta cagaaactcg attaaagagt ctatctctgc aattgaatct ttatgcagga
     1141 aaatcacggg caatgataaa ggaaccttgg gggcttgtct taaagctata gaggaaaaag
     1201 gatatataca ctctgcaatg aaaggcgctt ttagtcagct ctatggttat acaagtgatc
     1261 agggtggaat acgtcatgcg ctaacagaag aagatgtaaa tcctacctta gctgaagcta
     1321 aatttatgtt agtcacttgc agtgcgttca gtaattatct gttgtcaaaa ataagtgatt
     1381 aattacaggt ttatagttac gagtgggtta gttacccact ccctttaaaa ttattcacca
     1441 gactctaatc ttttttctct tttctcatct ctccttttta ttgccaaatc gtgttgctct
     1501 tgcagatacg tgcgttcttc gtttctatcg cttgaagtat atacgtctgc ttttactttt
     1561 ttagctcctt gtgcaatagg tttccaatcc tctcttatac ctttaatttg ttctgcaatt
     1621 tgcgtcaatg ggcttctcgc tcctagataa cttattcctt caaatgtcgc catgtcatac
     1681 acatattcat ttgtttctct atcgtttgat ttaggtttgc ttgcccagct tatattaata
     1741 ttataaattt gatctttgat ttgttcatac ttacagagat tgctggtaag tgattgccca
     1801 ttttttaaca cgcttacctt actaaacgga atctcacttt tttctctatg ctccgcattt
     1861 actagtggag gatcaaacac aacctcaaca ttataggcag gtgcgttccc tgtattagca
     1921 atgttaatat caaagtaaaa agcggcccaa gggtttggct caagtgttac aactatatgg
     1981 ggttgagctg atgcgtcaac cattcttttt gtctcgtctg ctctgaagtg gtgaagtggt
     2041 ttactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa cacaggtgct
     2101 ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg aatccgctca actggttgtt
     2161 gaccagacat acacggtggc agatgccgcc aaagctatgg atgttggcct ttccacaatg
     2221 acaagatggg tcaaacaact gcgtgatgag cgtcagggca aaacaccaaa agcctctccg
     2281 ataacaccag aacaaatcga aatacgtaag ctgaggaaaa agctacaacg cattgaaatg
     2341 gagaatgaaa tattaaaaaa ggctacaacc gcgctcttga tgtcagactc cctgaacagt
     2401 tctcgataat caggaaactc agagcgcatt atcctgtggt cacactctgc catgtgttcg
     2461 gggttcatcg cagcagctac agatactgga aaaaccgtcc tgaaaaacca gacggcagac
     2521 gggctgtatt acgcagccag gtacttgaac tgcatggcat cagccacggc tctgccggag
     2581 caagaagcat cgccacaatg gcaacccgga gaggctacca gatggggcac tggcttgctg
     2641 gcagactcat gaaagagctg gggctggtca gttgccagca gccgactcac cggtataagc
     2701 gtggcggtca tgagcacgtt gctatcccga atcatcttga gcgacagttc gccgtaacgg
     2761 aaccaaatca ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt
     2821 acctcgccgt tgttctcgac ctgttcgcaa gaaaaccagt gggctgggcc atgtcgttct
     2881 cgccggacag caggcttacc atgaaagcac tggaaatggc atgggaaacc cgtggtaagc
     2941 ccgtcggggt gatgttccag gcgatcaagg cagtcattat acgagcaggc agttccggca
     3001 gttactgtgg cgataccgga tcaggcagag tatgagtcgg cgtggaaact gctgggataa
     3061 cagcccaatg gagcgcttct tcaggagtct gaagaacgaa tgggtgccag cgacgggcta
     3121 tgtaagcttc agcgatgcag ctcacgcaat aacggactat atcgttggat attacagcgc
     3181 actaagaccg cacgaatata atggtgggtt accaccaaac gaatcagaaa accgatactg
     3241 gaaaaactct aacgcggagg ccagttttag ttgaccacaa cagactacct gaagggagcc
     3301 gcggtcgcct agcagttgca gtagcaggag atcatccagc cgcagtacag gtcacgatga
     3361 ctctggttaa tgataccggc tttgaccccg tattttccgg ctctatcgct gaatcatggc
     3421 gtcagcagcc gtgcacacca tcctattgtt gtgactggga ggctgccacc atgcttcgcg
     3481 ctttccctct ggcgaaaaag ggagaaggac gggcccgtct gccttcactt tatgccagct
     3541 tcggtaagct gggtgagaca ccgactcata aagatatcat tgataacaat cgatccatca
     3601 actggcctgt ataacgtggc tgccggtgat taagaaagct gcacctacct aagtagtagc
     3661 aaacgcacac tttttagaaa aatcgatggt cagaaactgg attagcaatt ccgttccagg
     3721 gttgcttttg atttacgttg gcgtctgatc attgatttat cctcaaaagc ccaacctcat
     3781 tggtaatgaa ccagctccgt gaatgtccgc tctggcacag agcgaaattt tttgatctcc
     3841 ccccctgaaa tctaaactta gtcatgtcac gtttttgggt ttctaaaatt ttaacttcgc
     3901 gtttttcgtt gccgtaaggg ttatacagaa atgtccgtta agcagagttc aaaattgatt
     3961 gccgtgatca cgactggttt gaaagccgcg cccaagcctg tacagctctg gtttgcgttg
     4021 attatgaacc tgtcagccta aagcaagcgg atggacgatg agtattggta atctttcaga
     4081 gtccggaaaa gttcagcccc agtctgaaca ggcttgctgg cgccagtcca gtttcattca
     4141 gtcgtggttt ggttcttacg gcctgtgcaa tctacctcat taggcacatc ggcctgccag
     4201 ataccggctc ggggtgtatt tccgcttcca cgctgaatac tgttctcagc aatcctgggg
     4261 tcatcacctc ttctggtgtg ccttgcgcca taacatgtcc gtttgccatt accaccagtt
     4321 gatcgcagta ccggctagcc tgattaaggt cgtgcagcac agcgaccacc gttttcccct
     4381 gagtccggag ttcgcccatc aaccgcatca ggtccacctg gtgattgata tcaagatagg
     4441 tggttggctc atcaagtaat acaacgggcg tattctgggc caggaccatc gccagaaatg
     4501 cgcgctggcg ctgaccgccg gaaagctcgg ttaaccgacg aacggcaaga tgattgatcc
     4561 gggtctggtt catggcgaca ttaactcgtg cattgtcttc agcggagaga cgcccccaga
     4621 gtgacagcca gggattacga ccatacgaaa ccagctcctg gactgtgatc ccctctggcg
     4681 ttaaatggtg ctgaggcagc agcgaaagcc tgcgggccaa ctggcgcgat gagagcatat
     4741 ttatgggatt atcgccgaga aatacggtgc cagactgcgg cattaaaagc cgcgaaaaac
     4801 agtttaacag cgtcgatttc ccgcaaccgt taggaccgat cagggcggtg atcttccccg
     4861 ttggcagtga gagtgaaacg tcgttaagta ccttgtctgt cccgtaactg accgtcagat
     4921 tttcagttcg taaagtcatt tatcgcattc tcacaagcaa ccagacaaac cacggcgcac
     4981 cgataatggc ggtcagcacg ccaaccggga gctccagtgg gggatgaata attctcgcca
     5041 gcagatcggc aaccaccaac agcaacgcac ctgtcagggc tgaaacaggc agcagtctgc
     5101 ggtgacgtcc accggtgatg ctacgcatca tatgcggcac cacgagacca ataaagctaa
     5161 tcgggccgca ggcggccacg ccggtagatg tcatggcgac agctagtaac aaagcccaga
     5221 atcgggtatg gggcaccgac acaccgagcg tggtggcgcg cgcatcgccg agtgcaagga
     5281 ggtcgagatc gcggcaaaaa ctcaggctca gcggcagaaa taaaatcatc agcgggatgg
     5341 caatcttcac aaagctccag tcacggcccc ataagctgcc ggtcagccac agcagggcgt
     5401 tgttcacatc ctgtgggcgc gagagcatca gataatccgt caggctggcc cagcatgcag
     5461 aaagcgccac gccggtgagc gccagcttca tcggctggtg ggtctttgcc agcatcttca
     5521 gtaatatcaa ccccgccatg ccgcccgcaa aggccagcag cggcagcacc atcacgggca
     5581 gtgacggcat aagaagtaga gcccccacag aggccaggct ggcggcatgg ttaacgccga
     5641 gaatatccgg tgatgccaga gggttgcgca caatcccctg tatcagcacg cccgccacgg
     5701 cgagggctgc accgacaaac agtgccagca gcaagcgcgg cagtcggtac tccatcaata
     5761 cataataatg ctcgcgtccg gcctgccagt cggtcagcag cgcgcgccac ggcacgggga
     5821 tcactcccat atggagtgat aacagcgcac agcccgccag ggcaagggtg atgaaaataa
     5881 ccagcgcaat tttcatcctc gcctcctcac aagccagaca aagcaagggc tgccaatcag
     5941 cgccagcact gcgcctgcgg gcagatctcc ggggaaggcc agcgcgcgtg cgagtacatc
     6001 tgccagcagc atcagcgtgg cccccagcag catgctcact ggcagtacgt tgcgctgatc
     6061 gaagcctgcc cagaagcgcg ccagatgtgg caccagcaga ccgataaacg ccaccggacc
     6121 tgccacgctg acgcacgcac caaccagaag cagcactaac atattgatga ccaaacgtag
     6181 cctcgtcagg ttcactccca gcgtatgggc ggtgctgtcg ctgaggttga gcaggttcag
     6241 ttgattcgcc agcagcaaca cgacagggac tgcagtgacc accaccggca agagctgcca
     6301 gacatcctgc cagcgggcgt gggacactcc gcctgccagc cagtaaaaga tgccgtaagc
     6361 atgatcttcg gccagcagca gggtgatgcg ggtaaggccc atacaaaagg ccgacagcgc
     6421 gatactcgcg aggatcagtt tgtttctgtc atgggtatga cgaaatccgc ctcctgcggt
     6481 catggccagc agccagctca cgccgccccc gcatgccgcg atgaacgaca gagaatagcc
     6541 tgcaatcggc gtcggactca gcgcgctggt aagcgccata gccagcgccg cgccgctgtt
     6601 aatgccgagc agtgaaggag aggccattgg gttgtgggtc agggtttgca gcagcgtgcc
     6661 cgcgagcgcc aggcttgcgc cgatcagaac ggcgaccagg cttcgtggca aacgaaggtt
     6721 ttgcaccagc gcttctggta gcgttggcgt gtgtccaggc agcagggcgc gggttgcatc
     6781 tgctccggaa acaggaatgg ccgagtagca aaacagactc agccagaaaa taataataag
     6841 tgctgcaacg ggaagccccc acagcagcac cgggtgtttt atcgcggtca tttcacaacg
     6901 gtaagcggct gatggtggaa gatttttacc gtgtcagcgg caatacgctc tgcagcaaaa
     6961 ataccgcgca tccgcgccca ggtgttactg tcgaccgaag caacctgctg cttctgcgcg
     7021 gcggttaaca tctgccagag cggatcttgt tgccagcgtt taacaatgct ctcttcgcga
     7081 tagtgggcaa ccagcagcca ggcaggattg accgccagca gttgctccag gccgatggac
     7141 ggcatggacg cacccgccat cgcagcggga acgttcagcc ccagagaggc cagcacgctg
     7201 ccggtccagg tctcctgagt atgcaggttg aattgctgtt cgcgtgatgt gccaaaggcc
     7261 acgcgtgtcc ctttgggaag ctggctggcc cactgcgcca tcctctcttt atgttgttcc
     7321 aggcgtgcct gcatctctcg ctttttaccc accatttcgc cgatgatagc cgcagattgc
     7381 aaattttcag cgtaggtttc gttgcgggac ttaagcagca gtaccggcgc gatttgctgc
     7441 aaggcgatgt aaacccccgc atggcgactg ctgtcggcaa tgatcaggtc tggtttcaga
     7501 gcggcaatgg cttccaggct cggctgcgcg cgcgttccga cggactgcca cggtttcagg
     7561 tgcgcacgca cttcgggcag gatgcgtttt gcatcgttat cgtcggcaat accgatcggg
     7621 ctgacgtcca cggcggccag cgcatcggcg aacgagagtt ccagcaccac aatccgttgt
     7681 ggcgtttttt cgagtgtaaa cgtgccgtgt tcgtcctgaa ccgtggcggc aaaggcgtgg
     7741 ctgatcacca gcagcaggcc tgcaaaaaca aaacggataa atgccaacat aatcacattc
     7801 cagctaaaag cccggcaagc cgggcgttaa cacatcagaa cttcaacgac ccctgcatat
     7861 acagcgtgcg cggctggcct gcatagatgc ctttgttgtt gtcgtcataa gagcggatga
     7921 agtagtcctg gtcgaagatg tttttcacac cgaatgccag gttcagatct gccatctgcg
     7981 ggccaaaatc atacgccacg cgtgcgcccc agagcatgaa gccgggaatg cggccggtac
     8041 tgccgtcggc gctctctttc accgtattgg cgttatccgc aaactggctg gactggaaat
     8101 cgctgttcag attgaacgtc cagtttcctg gcttgtagtc cacgcccagc gtgcctttat
     8161 gtttcgggga gaatggtacc agattgccgt aggtgtcgcc tttctcgcgg atttccgcgt
     8221 tcacatacgc atagctggcg tagatggaaa cgttatcaag cgttggcgtt agcgtaccca
     8281 gatcgtaacg tgcctgcgtt tccagcccgg tatggcgcgt tttgccacgt gcagtgacgg
     8341 tgtcgttggt ctggttggag tcgtactgat tgttaaagtt aatcaggaac agccccattt
     8401 ccgccgtcag cgcgccgtcg tcgtagcggg taccgagttc ccaggttcgc gctttttccg
     8461 gttccacatt gccgctttgc acagccttgc caatctggct gtactgtacg gtgccgaacg
     8521 agccttcagt gtttgcataa agattccagc tgtcagtcag gtgatagagc acgttcaacg
     8581 ccggaagcgg tgcgttatag ctcacttctt cgtgcgtgcc tgtgatggcg ttgttctggt
     8641 atgactcgat atgttcgaaa cgcatacccg gcgtgatggt ccagttgccg atgtcgattt
     8701 tgtcatccag ataccaggcg tgcgcctcgg tgccggaacg cgtatcgcgg tcgtaagggc
     8761 ttgagccgga cggcaactgc ccgctgctgg tggcggtgta gtaacgcatt tcatgcgttg
     8821 attcattcaa atagcgatag cccacgccca cttcgtgcgc ggaagggccg atcataaaga
     8881 tctggctgta gcgtggctca ataccgcgca cccagtagtt acgcggcgag agggtgatgc
     8941 gtttgccttg ctccaggtag ccgctgcgca gggtttgggt gtagaacccc tgaatgttga
     9001 atttatgctg gctgtctggc tggaactggt agcccaggct cgccagcttg cgacgacccc
     9061 agaagcggtc atacgggcgg gtggattgcc agcgatcggc gtcgtaatcc gcgcgagaca
     9121 ggccaccggg catgtcggct tcaccatcgt aatattgcag caggctgttg aaggtgtgca
     9181 cctcatccgg cgcatatttg cttttcagca tcaggtcgtc gatgcgggtg gcgctgtgct
     9241 cgcgccagtc actgccgcgc gtgccggagt agagcagcgc ggtgccaaaa ccgttgtccg
     9301 ctgtgccgcc caccatcagg ttgtgcgtct ctttcgggtt gttttgtgaa gaggttgggc
     9361 tgagctgacc ttccacgccc gcctcgatac caaagtcctg cggaatggca cgggtaacaa
     9421 agttcaccac gccgcccacg ctctgcggtc cgtaacgcac cgcaccacca ccgcgtacca
     9481 cgtcaatggc atccatgttg ccgagcgaaa cgggagccag tgaaagctgc ggctgaccgt
     9541 aaggggcgaa ggggacgggg atgccgtcca tcaggacggt cgagcggctg gcgaggcgcg
     9601 ggctcaggcc ccggatgcca aagttcatcg ccaggtcgtg gctgccggtg ccgttgtttt
     9661 ccggcgcgct gacgccaggg atgcggttaa gtacctcacg catggtggtt gcgccggttt
     9721 tggcgaaatc ctcacggcgg atcacgtcac gcgcgccagc atgttcaaat acgtcgtttt
     9781 cacgcgcatc acccagccag tcgccgacca cggtcagggc atcttctttt ggtgcgggcg
     9841 cgggctccag cgtccagctg ttatttccca gcggttttac ctgcagtccg ctgccgtcca
     9901 gcagttgttg caggccgctc tcgacgtcgt aatcgccgtg caggccgttg ctctgcttgc
     9961 cgcgcgtcag gctggcgtca accgagaggg taaatccgct gtgtgcggca tactgattga
    10021 gcgctttatc gagcgatccc ggtgcgatat taacctgtgc agcaaaagcg gaaaacgaga
    10081 gaccggccag cggcagcagg ctcaggcgaa tggcgttaac caaaggtgtt gttttacgaa
    10141 aaacgcgtaa cggcgtcata ccttccccat catcattttt gttgtgttca gctatgagtc
    10201 gaacgagaag ggtaaaaagg acaatcgaaa taagaattat tttccttaca gtggtgaaat
    10261 gtttatccag taccgcgtaa tagactgaat tttaacggga agcgtttgcg cgataacgtt
    10321 caggatcgca tcggtatttt tcagcgggaa cgtcccgctc aggcgcagcc cggcaacggc
    10381 gggatcgcag cgcagcacgc cgttgcggta acgggttagc gtggctatca cctcacccag
    10441 cggtttatcg ctgaagctca ggatgtcctt cgtccagctt gtactctcgt catccagcgg
    10501 tttcactgcg ccaaactcag aggcgctgaa ctgcaggctt tcaccagcgt tcacgatgcg
    10561 tttttgcgcg ggggcactgg cgagaagcac ttccacagcg tgctgctgca cgtcaagctg
    10621 cgtgaaatta tcctgctggc ggacggtaaa ttctgtccct aaagcagtga gctggccctg
    10681 acgggtcagg acacggaagg ggcgttgcag ggcatctttc gcggtagtaa tggcgatttc
    10741 accgtaccag agccggacgg tgcgctgatg cgcatcaaaa cgcacatccg ccgcgctttg
    10801 ggtattcagc gtgagcaggg agccatcttc cagttgctga cggctgactg tgcctttggc
    10861 ggtgcggtaa tctgcccgca gaccttcgcc ggtttccgac tgccagagct gccagcctcc
    10921 gccagcgccg agcaacagca gtaatccttt catcacgtga cggcgggtga ggcgggtatc
    10981 gtgcaacgcc ctgctggcta cgtcgccagg cacaccgcca agctggttgc gcaggttttc
    11041 aacctgctgc caggcccact ggttatcctg atcctgttca taccactgtt gccagcgcgc
    11101 ttcctgttgt gggctgacgc gctcgccgct tagcacggca taccagtggg aagctgaacg
    11161 cagcgcctga cggcgggaat cggttaacaa aggattcata acccatactc cagacggaac
    11221 agcaggcagt gctcgacggc tttcgccacg tattttttca cggagctgat ggaaacaccg
    11281 agtttgtgcg caatctcgct gtatgtcaga ccatccagtt gcgaaagcag aaacgcttca
    11341 cgtgttttgc cgtttagccc gtccagcatg ctgtcgagga gttgtagggt ctcgagttgg
    11401 ctttcgcgtt cctcaggtga aggcgctccc ccctccggca taagcgccag catctccaga
    11461 tacgcttttt ccagcgcgtt tcggcgaaac aggtccacca tcacgcgttt ggcgatagtg
    11521 cagaggaagg agcgaggatc gcggatcgtc gagagcgttt cgctgaccat tacccgcaaa
    11581 aaagtgtcct gggcaatgtc atctgcatca aaagcagact ggagtttgcg cgtcagccag
    11641 cttttcaacc agccgtgatg tgtgccataa agcgactcga acgttaagga agctgtggta
    11701 gtggcgcggt cagacatgcg gagtgcatca aaagttaatt atcacgtagt catattaata
    11761 tgagaatggt tatcattaca attggaaata aaattgtttc caatagacat ttttaacatg
    11821 ttgtttttct aagtgttata aggtaggtat aaaatgggat ggagcctctg cttctggcat
    11881 gtgtcggtca gaatgactca tgatgtggtc tgctattatt gacatcctca ctgccctaaa
    11941 ggatggggat ttcggtaatg ctgccaactt actgatttag tgtatgatgg tgattttaag
    12001 gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact gaaggcgtgg
    12061 tgcgtaacgg caaaagcact gccggacatc agcgctatct ctgctctcct tgccgtaaaa
    12121 catggcaact acagttcact tacaccgcct ctcagcccgg taagcaccag aaaatcattg
    12181 atatggccat gaatggcgtc ggatgtcgcg ccagtgcacg cattatgggc gttggcctca
    12241 acacggtttt acgtcactta aaaaaatcag gccgcagtcg gtaacctcgc gcatacaacc
    12301 gggcagtgat gtgattgtct gcgctgaaat ggacgaacag tggggctacg tcggtgctaa
    12361 atcacgtcag cgctggctgt tttacgcgta tgacaggata cggaggacgg ttgtggcgca
    12421 cgtcttcggt gaacgcactc tggccacact ggagcgtctt ctgagcctgc tgtcggcctt
    12481 tgaggtcgtg gtatagatga cggatggctg gccgctgtat gaatcacgcc tgaagggaaa
    12541 gctgcacgtt atcagcaagc gttacactca gcgcattgag cgacataatc tgaatctgag
    12601 acaacatctg gcaaggctgg gacggaagtt actgtcgttc tcaaaatcgg tggagttgca
    12661 tgacaaggtc atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac
    12721 ctcctcccga actcaaagct atcatacaga taattactgg ttaaccctat atagggcatt
    12781 ttaaagtgtt gtgatcttac ccagcaatag tggacacgcg gctaagtgag taaactctca
    12841 gtcagaggtg attcacaaga caaaacagtt tcaaccagta aaaaaacgta aacaccattc
    12901 gcatgaattt cgtagttaag atctgaagct tgcagaatgc atcggtgtta ctgccggagt
    12961 tcgtgaactt agtctgcatg aatcaccaca gctttacatc tgtcgcagta aacagcaaaa
    13021 tcagcaaact tacttaatta tatcgagtgc gattacaatc gctggcgatg gtacagttgg
    13081 tgtggcggtc tcagtccgga acaatttgaa aatcagaatc tcacttaaaa cggcatccat
    13141 tttacaaaag atggccgtct gtgtatttgt aatgaatacc ttctacatgt ttagagtgca
    13201 taatccacga caaggcgata tcctaaaaat tagctagcag gcaactttga tggcatagat
    13261 aatgttttta gtaaaaaaga gttgactgaa tataatacag cctcttccta cataagaagg
    13321 aatgtgctca caaaaaaaaa ccattagtct gcttcctgtt cttacaataa tcagtattat
    13381 gtattttatt tatatagcaa aatatgcttg ttacacctgt cataattagg catggtaaaa
    13441 ttattgactg aaccctgggg gagaaagatg cacagtgatt attataatat ggtttttggt
    13501 gaaaaattgg ccaatatttt atatgaagct aactctcaat ttttttacga gagaaatgtc
    13561 atagaagagg cagtcaacgc tttattctgt gagcgtgaaa taataaacaa caagaacata
    13621 ataaagaagc tgatgttctt tttgtcagat gttaatcaca ccaagaaaga tgttgttcag
    13681 agcgcactta atattatcat tgacattaca tcaggtgata tttgagattt tacataaaag
    13741 ataatctctc aaggagtctt atctgaaaac gaaatatata gtccctctcg ttttgtaata
    13801 tcgattggct ttgttgaatc ggttacgtac cccggagccg gtcatatttt caggtaatac
    13861 acacgctttc agacatttcg gctttcgtca ttttggtatc atgggcatag cttctgctac
    13921 ctgaccatca taatcacgca gcttcagtga agcagtgacc acctgtttaa cccggtgtat
    13981 cgttatgatc ttatccagca attgaaccgc cccgggtttc ctggagagtg ttttatctgt
    14041 gaactcaggc tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg
    14101 ccggtggaat atggccctgc ctttccagca atcgtcgatt gttataccag tccacccacg
    14161 tgagtgtggt cagctcatca tactgacacg tgcattaccc ctaccgcgat aatgcaaccg
    14221 tacaatatag ggcgtttcct aaacgcagcc ctcatcactc caatccaaca agacacagca
    14281 tttttgatca cgaagaaaga caaataattt ccgtaaatgt ttccgttgtt acaaataaca
    14341 acagtgtttt atatttcttt ggcctcaggc ccgcgcatgt tgccattcga tgtcattttc
    14401 ctgactcgtt tcttctttat ggtgtagtgt gatcttcact ttatggatgc taacaatatg
    14461 aaaagaaaaa ttattcccgt gctaatcggt tgtacgctct ctttctctgc cctggcggca
    14521 caacctacgg ctgagcgcta tgtcgtcagt tttccagaag gcacgcacgt gaactatgcc
    14581 ggcgcatttg ccagtgcatt cccgaacggc cttccggttg ggataggttc tggcctgctg
    14641 ttcaccggta aacagggcga tgccctgaca ttcgcaacca ttaccgatcg cggtccgaac
    14701 gcggattcac cgaaagaggg gaaaaacgaa actaaaattt tcgttacgcc agatttcgcg
    14761 ccgctgctga tgacgattcg tgtacaaaac ggcaaagcgg aggccatcga tccccgtcct
    14821 ctgcatgacg ataaaggcgc gatcaacggt ctgccgctgg caagcgacgt gattggctcc
    14881 accaatgaag tggccttcag cgacacgtta catagactga agggggataa tcgcgggctg
    14941 gatacggaag ggatcactcc ggatggtaaa ggaggttact ggctgtgcga tgagtatggc
    15001 ccgttcctga ttaacatcga cagtaaaggg aaaatcctgg caatccacgg tccgcaggcg
    15061 gcggaagggg aaaaagcgat tgcgggcggt ttgccaaata tcctcaaatg gcgtcaggcg
    15121 aaccgcggct ttgaaggcct cacccgtatg ccggacgggc gtattattgt cgccgtgcaa
    15181 agtacgctgg atatcgacgc taagagcaag aaaaaggcgc tgtttacgcg tctggtgagc
    15241 ttcgatccgg caagcggaaa aaccgcgatg tacggttatc ctatcgacag cgcggcctac
    15301 agcaaaaaca gcgacgctaa aatcggagac atcgtggcgc tcgataacca gcacatcctg
    15361 ctgatcgaac agggaagaga taaaaacaac agaatgcgta acctgattta cgaggtcgat
    15421 ctcaataagg cgagcgacct gtcaggcttc gacaagccgg gtgagtatcc ggagtttgat
    15481 gatgagaaaa ccctgtcaca gcgcggcatt acccttgcgc agaaaacgca ggttgttgat
    15541 ctgcgctcct tgggctggca gcaggagaag gctgaagggt tggcgctgat cgacagcaaa
    15601 acgctggcgg tggcgaacga taacgacttt ggcgtgaagg tggcgatgca acatccggtc
    15661 gagggcaaga cattcaagga ttatcgggtg aacgcggaag gaaagctgac gcttgacgac
    15721 aagcaggttg aaacgaccct cagagtgaag ccactggaga aaccggagtc tgacagtgaa
    15781 ctgtggattg tgacgctgcc ggaagcgttg aaataagttt ataaggtacg gacagcagta
    15841 tcgcttagtt tttcaaattc atgtatgtga acaatattgg ggttccggcg tagaccccca
    15901 atgaaagcgt caccacgtga aaacatgcct gcacggttta cacgtcatct catggtggca
    15961 gccggttaac tttcctgcta caccagtgga aaaaggccat aagcagtccg aacaaa
//
LOCUS       DAZFKM010000111        15816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFKM010000111 DAZFKM010000000
VERSION     DAZFKM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            146..688
                     /gene="yfaZ"
                     /locus_tag="ACHKAY_003705"
     CDS             146..688
                     /gene="yfaZ"
                     /locus_tag="ACHKAY_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO5702308.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            788..1990
                     /gene="yfaY"
                     /locus_tag="ACHKAY_003706"
     CDS             788..1990
                     /gene="yfaY"
                     /locus_tag="ACHKAY_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO5702309.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2054..2515
                     /locus_tag="ACHKAY_003707"
                     /pseudo
     CDS             2054..2515
                     /locus_tag="ACHKAY_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2556..3458)
                     /gene="rpnE"
                     /locus_tag="ACHKAY_003708"
     CDS             complement(2556..3458)
                     /gene="rpnE"
                     /locus_tag="ACHKAY_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO5702310.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3652..4842)
                     /gene="glpC"
                     /locus_tag="ACHKAY_003709"
     CDS             complement(3652..4842)
                     /gene="glpC"
                     /locus_tag="ACHKAY_003709"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO5702311.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4839..6098)
                     /gene="glpB"
                     /locus_tag="ACHKAY_003710"
     CDS             complement(4839..6098)
                     /gene="glpB"
                     /locus_tag="ACHKAY_003710"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO5702312.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6088..7716)
                     /gene="glpA"
                     /locus_tag="ACHKAY_003711"
     CDS             complement(6088..7716)
                     /gene="glpA"
                     /locus_tag="ACHKAY_003711"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO5702313.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7989..9347
                     /gene="glpT"
                     /locus_tag="ACHKAY_003712"
     CDS             7989..9347
                     /gene="glpT"
                     /locus_tag="ACHKAY_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO5702314.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9352..10428
                     /gene="glpQ"
                     /locus_tag="ACHKAY_003713"
     CDS             9352..10428
                     /gene="glpQ"
                     /locus_tag="ACHKAY_003713"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5702315.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10470..10709)
                     /locus_tag="ACHKAY_003714"
                     /pseudo
     CDS             complement(10470..10709)
                     /locus_tag="ACHKAY_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10707..10811
                     /locus_tag="ACHKAY_003715"
                     /pseudo
     CDS             10707..10811
                     /locus_tag="ACHKAY_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10891..11541
                     /gene="inaA"
                     /locus_tag="ACHKAY_003716"
     CDS             10891..11541
                     /gene="inaA"
                     /locus_tag="ACHKAY_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO5702316.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11595..11849)
                     /gene="yfaE"
                     /locus_tag="ACHKAY_003717"
     CDS             complement(11595..11849)
                     /gene="yfaE"
                     /locus_tag="ACHKAY_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO5702317.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11849..12979)
                     /gene="nrdB"
                     /locus_tag="ACHKAY_003718"
     CDS             complement(11849..12979)
                     /gene="nrdB"
                     /locus_tag="ACHKAY_003718"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO5702318.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13068..15353)
                     /gene="nrdA"
                     /locus_tag="ACHKAY_003719"
     CDS             complement(13068..15353)
                     /gene="nrdA"
                     /locus_tag="ACHKAY_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO5702319.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRALGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15481..15624
                     /gene="ypaB"
                     /locus_tag="ACHKAY_003720"
     CDS             15481..15624
                     /gene="ypaB"
                     /locus_tag="ACHKAY_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO5702320.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3681 a         3900 c         4305 g         3930 t
ORIGIN      
        1 attttttcgg cgtttaatta tttaagaagt atttagttac aataagtgac tagatccctt
       61 ttttcgctca ttgaatcctc tgtgccaggc agtacaatcc cgcagctttg ggaattttga
      121 ttaattgctt gtgggaaagg ggataatgaa aaaaattgcg ctagcaggtc tggccggaat
      181 gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc ggccaggcgg gtaaagaata
      241 caccaatatt ggtgtcggtt ttgggactga atcgacgggc ctggctttaa gcggtaactg
      301 gacacataac gacgacgacg gtgacgtcgc gggcgtgggg ctggggttga atctgcctct
      361 cgggccgtta atggcgaccg ttggcggaaa aggcgtttac accaacccga attacggcga
      421 tgaaggttat gccgcagcgg taggaggtgg tttgcagtgg aaaattggca acagcttccg
      481 tttgtttggc gagtattact actctccgga ttcgctctcc agcggtattc aaagttatga
      541 ggaagcgaat gctggcgcgc gttacaccat tatgcgtcca gtcagtattg aggcgggtta
      601 tcgctacctg aatctgtcgg gtaaagacgg taaccgcgac aacgccgtgg ctgacggccc
      661 gtatgttggg gttaacgcca gtttctgatc ccttccggcg cggcattctg tcgcgccatt
      721 cagtttctta ctacgcctta tctccctttg cgttatagtg attcgataac aaaagcagga
      781 gataaccatg ttaaaagtgg aaatgctatc caccggggat gaagtgttac acgggcaaat
      841 cgttgacact aacgctgcct ggctggccga ttttttcttt catcaggggc tgccattatc
      901 tcgccgcaat acggtggggg ataaccttga tgacttagtc accattcttc gcgaacgtag
      961 tcagcacgcc gatgtgctga tcgttaacgg cgggctggga ccgaccagcg atgatttaag
     1021 cgcactcgcc gctgcgacag caaaaggtga aggcatggtg ctgcatgaag cctggctcaa
     1081 agagatggaa cgctatttcc acgaacgtgg acgagtaatg gcaccgagca accgtaaaca
     1141 agcggagctg cctgccagtg ctgaatttat caataacccg gtaggcaccg cctgtggttt
     1201 tgccgtgcag cttaatcgtt gcctgatgtt ctttactccc ggcgtaccgt cagaatttaa
     1261 ggtgatggtc gagcacgaaa tcctgccgcg tctgcgcgag cgtttttctt taccacagcc
     1321 gccggtttgt ctgcgtttaa ctacctttgg tcgttcggaa agcgatctgg cacaaagcct
     1381 ggacactcta caactgccgc cgggcgtaac aatgggctat cgctcttcaa tgccgattat
     1441 cgaactgaaa ctcaccggac cggcaagcga gcaacaggcg atggaaaaac tgtggctgga
     1501 cgttaaacgt gttgccggac agagcgtgat tttcgaaggc accgaaggac tgcccgcgca
     1561 gatcagtcgc gaattgcaaa accgccagtt cagcctgacg ttgagcgagc aattcaccgg
     1621 tggtttattg gctttgcaac tttctcgcgc aggtgctcca ttgctggcgt gtgaagtggt
     1681 tccttcacag gaagaaaccc tggcgcaaac tgcgcactgg attacagaac ggcgggccaa
     1741 ccattttgcc gggctggcac tggctgtttc gggtttcgag aacgaacatc tcaactttgc
     1801 gctagccaca ccagacggca ctttcgctct gcgtgtgcgt ttcagcacta cgcgctacag
     1861 cctggctatc cgtcaggaag tgtgcgcaat gatggcactg aatatgctgc gccgttggtt
     1921 aaacggccag gatatcgcca gtgagcatgg ctggattgag gttattgagt ccatgacctt
     1981 atctgtctga ttccgccact ctgtgccggt ttactgcacc ggcacattcc ccctccggca
     2041 aaacttgtcc aaccctccct acggtgagcg tggtgttggg gccagtgtgg cacgggctgc
     2101 gcgctgggga cgcattgaga attacatggc gcaagtcaac gattcgcttt gtctgttagt
     2161 gcaggtggaa agtaaaacgg cactggataa cctggacgag atcctcgacg tcgaagggat
     2221 tgatggcgtg tttattggac cagcggatct ttctgcgtcg ttgggctacc cggataacgc
     2281 cgggcacccg gaagtgcagc gaattattga aaccagcatt cggcggatcc gtgctgcggg
     2341 taaagcggct ggttttctgg ctgtggctcc tgatatggcg cagcaatgcc tggcgtgggg
     2401 agcgaacttt gtcgctgttg gcgttgacac gatgctctac agcgatgccc tggatcaacg
     2461 actggcgatg tttaaatcag gcaaaaatgg gccacgcata aaaggtagtt attgatatca
     2521 aaggcccatg gggatcggct gtgggcctgt gttaattagt ggttattcgc tgccagatcg
     2581 gcttcgctta gctgggtggc cgcgagcacc tggtcacgat caatgccctg ttccagcatg
     2641 cgcaaagcaa ttttaatggc ttgttcatgc ataccttcct gccagccaat ttgatgccct
     2701 tcctgccgta gccgttcagc aatagtcatt aatctctcct tttgtagtgg tgaacgttcg
     2761 gcaatctcct caataaaacg ggtgaaacgg gaggtatcgc cgcattgcag cagataatta
     2821 aacagtgttt gtagctggct gtcattagtg aagcctctaa ccaaaagcgt ggtgatcctg
     2881 tcgaccatgc cgattaaatc gcggtcgcga atatgctttt gaatcagttc cagcagagct
     2941 atacgccgat gttgcatgat ctcatcgtca ggtacgatgg tgatatccac caacggaaaa
     3001 gcttcggtgt acaactgccg ggcaagttgc ggatcgtcaa actcatccag ccagttaagt
     3061 gagtacgggt agggcgatgt ttcgccatga taaaacagca atggcaccac cagcggaact
     3121 ctgtcatagc ctttatccag gtgacgctgc atggcggcag tggcatagcg cattagccga
     3181 aaagccatat tcttttctgc agagctttga tgttcaatca cgcagtagat atagccgtca
     3241 ccgtcgctgg tttccacgga ccacaaaaca tccgagtagt aagcgcgtaa acttttttca
     3301 ataaaactag tgggttccag gcgtaaggtt tgcaggttgc aaagcttgcg cagtggttct
     3361 ggtaaatgta tttcgagaaa atcccgtgcg gtttcgggtg tgaacataaa ggttttaaac
     3421 accgcatcat gcggggagga tgttgttgat tcggtcatgg ttttccgtca cctgatattt
     3481 atgatgacgc gacattacgt ctatcccggg cctttgacat ctgtcagttt cagcctttgc
     3541 gagacatttt cagaggaatt ttcaaagcgt tgcaacgcat acatggtgcg tggaatcgcg
     3601 ctcgcaaaaa tgcccgataa gcaatgtgct taccgggcat caaaaggtcg gttaagccag
     3661 cgcctgggcc agtagcgtaa tcggatgttc gcagcgaagg ctcgtggaca tctcaatctg
     3721 ccatttgcag gtttcgcagt cggtgatgac cagatccgcg ccgctctctt ctatctggcg
     3781 gaacagtggc gcaccgatgg cttgtgaggt ggggtagttc tcttttttga aaccgtaagt
     3841 acccgcaata ccgcagcact gggaatccag caccgttaac tcaagccccg ggattttacg
     3901 caacagctcc agggtgtaga gcgtccagcc cattttttcc atatggcacg gagtgtgata
     3961 aaccactttc agcggcagcg gtttcagcgg taacgttttg ccttcgtcca gcttgcgcca
     4021 cagccagcgg gttgccagtt cgatatgatc gcgcaagcct ttgttgtcga cattcagcac
     4081 ttccgggtat tcgtcgcgca gggcaaatgt acaggttgag gaggtggcaa tcaccggaat
     4141 gccttttact cccacagctt cgcggatcga ctctacattc gtaattgcct gtttgcgtgc
     4201 tttatcggta aagccgttgg cgattagcgg tacgccgcag catttttctt tgctgagcag
     4261 ttgtacaccg gtacccattg cgttgagcac tttaattaaa tctttaccta actgcggatg
     4321 gttgtagtta acgaagcagc cgtgaaagaa agcgacctgg tctttatatt gtgcttgctg
     4381 agccgctatg ctgcgatacc agcgacgaaa cgtaccgaag gagtatttcg gtagcgtgcg
     4441 gcgatgatcg atttttaacg ccgcatcaag cagctgccgc accggtttca gcgaggtagc
     4501 ggtgttgacg attggtgcga acggcgtgga aacgctaccc atcaggtcgg tatgactcaa
     4561 cacaaaatta cgcagcgacg ggcgcgtggt gtcatatttt gcccgcgcgc gctggataat
     4621 atcgccaatc ttcacatcgg acgggcaggc gacttcacaa cgtttgcagt tgatgcaata
     4681 tttcagcgcc tcgtcataca gtgcgccatc tttcaaacgc agacgctcgc catccggccc
     4741 ggcttgtttt ggccctggat aaccgggatt cacccggctc accgggcagg cggtggtgca
     4801 gacggtgcac ttaatgcagt tttcgaagct ggtgtcgttc attgttggcc tcctgcgcgt
     4861 tgggcaatct gttgagcggc atgtaaagca ctgacggcac aaacaccgcc gccgcatccc
     4921 tgggcgatgg gatcaaatcc gcccagcacc gaaccgatgg caaacaggtt ctcaatggtt
     4981 tgccctgcct gcgacgggcg tagcgtctga tcagtggtta caccgaactg ctgccacggt
     5041 tgcggcgcaa aaaaatcgcc cttataccat tcaccccgcg tggcggtttg tagcacatca
     5101 aggccgagaa tcggctcgcg aatgccgtta cgttccgcta ccagtccgcc actaaagaaa
     5161 ctgccgctgg cgagaaccgc gaaacgtgga cgtagcggaa tatcggcgtg attgcgggtc
     5221 cagatttcat tcactacgcc atttttacag gtcacttttt tcacttcatc gcccggcatc
     5281 cacacgccac cctggcgcac aaactggcgc tgtaactggt tttgcagacg aatgcccagc
     5341 acggaaggcg gcagcgttgg caaaagcatc agtgaacaag gtagtttttc attcaaccaa
     5401 cgccacagtt tgtcatcggc caggccgaag caggcgggca tcaggatcat ttcgcaggta
     5461 ttggcgacag gaataagcgc atcaagtaac agcggccagt tttcttcatt atcaaggaaa
     5521 cgggcgatat tcaccgcgcg aaattcggtg gcgttattgc gcaggacatc cagttccggc
     5581 agctctattt ctgcggtttc aaccgaaaga tccagttcgc gcaacgaagc cgccgcaaga
     5641 tgcgcctgga aatccatcag cccgcttatc cccactacac atatcttctt cgcgggtagc
     5701 ggccagacgg gaacttctgg cgaacttaac caggttgcac gcaaagtgcc gagcggggta
     5761 acgcgctgat gggccagttc cacgctgccc tgcaattgcg caccgctttc agcgatcaat
     5821 gcctgcgcct ggcaagcgag atcgagcacg cgttgtggtt cgagaagact gtaaggatgg
     5881 gctggtgcct gctgacgcaa agattccagt ccactgtgaa tgtctgtcac cggttgacca
     5941 tcaggcagat ggctcagcaa atccagcgaa ccggatgaga aatgcagcgc gctttgacca
     6001 cgcgtgacaa tggcacagcg caggccgtgt ttttgcagtt gcaggccaca gagtaatccg
     6061 gcgaggccgc cgcccataat gacagtatca aagcgcatct ttctgctcct tctccagacc
     6121 acacaatccc tgataaaccc agcgggtaaa ttcgctttcg cgcagtgcat ctccccaggc
     6181 gatgggttgc acgcctttcc agcgttcgtt aaggaaggtg gaaagttgct cgatagattg
     6241 cgcggacgtc gtgacgttaa aacgttgcag cagtccggca gcgcggcagg cgcagagttc
     6301 gccctggcag gtacccatcc ccacacgggt acgacggcgt aaatccagca ggctattaac
     6361 gtttaaattt tctaccgcgt actgaacttc acccgcagtt accgcttcgc actcacatac
     6421 caggctacga tgcagacggc cttcgctcag ccaggcaggc gtgcgatcgc catgacgata
     6481 aaccgcagaa ccgcgcagcg gggcaggcag ggagatgact ttacgcaagg taacttcagc
     6541 gggttcttgt gaaccaggca gtgccagatc ggcagtcgta caggggcgcg tgttgcccag
     6601 tttgcggcat accgcgtcgg tagcccattc agccatcagc cgataggtca tcagtttgcc
     6661 accggtgatg gtgataaatc cgtccagacc atcgcgttca gcatggtcga gcagcacgat
     6721 gccacggctg acgttacgtc cgctcgggtc gtcatcgctg gcaaccagcg ggcgcacgcc
     6781 agaataggcc cgcaaaatgc gcgttttcgc catcacgggg gccagttttt ccccttcacg
     6841 cagcagaata tcaacctctt ctgccgtcac tcgattatcg tcaatctcgt tgtaatcaat
     6901 acgtaaagag gtggtgccaa tcagcgaaat ggtatcgcca ggcaccagaa tatcggcgtc
     6961 ggaaggttta cggcagcggt tgatcacatg ctggttaatg cggtgatcca tgatcagcag
     7021 cgatcctttc gccgggaaca tgcgaatgcg cagatcggca tattcggcaa tgtgttgccc
     7081 ccagatccca gcggcattaa ccacgacagg tgcatgaagg gcctgagttt cgccggtgag
     7141 atggttacgt acacgaacac cgcacaccgt cgcgccttca cgaatcagcc ccgtgacttc
     7201 atgagcggta aggataacgg caccgtgttc tttggcatcc agcatgtttg ctgcggtcag
     7261 acgaaatgga tcaacggtgc catccggaac tttcaccgcg ccaatcagtg ccgggttaac
     7321 ggcaggttcg ataatgcgcg cttgctgcgg gtctatagct tctgcgctga tccctgcttc
     7381 ttcgcaggcg cgaataaaag tggcctggaa tgagaggtca tcttccggca gggtgataaa
     7441 caggccgttg gttggttcaa cgcagtgacg tgcaatgcgt ttcaggatct ggttttcact
     7501 aatgcattcg cgggccgatt ccgcatcggt taccgcatag cgcgcaccgc tgtgcagcag
     7561 gccgtggtta cgcccggtgg caccggttgc gatgtcgtgg cgctcaacca aaatcacgcg
     7621 cagcccgcgc agggcacagt cgcgggcaat cccggctccc gttgcgccgc caccaatgat
     7681 aatcacgtca cttgattgcg agtcgcgagt tttcattgtt atccctctga agttcgtttt
     7741 ttaccattta gccatagtaa aaacatgaat tgtttgattt cgcgcatatt cgctcataat
     7801 tcgaaagtga aacgtgattt catgcgtcat tctgaacatt ttgtaaatct tatttaataa
     7861 tgtgtgcggc aattcacatt taatttctga atgttttctt aacatcgcgg caactaaaga
     7921 aacggcaggt tctcccactg aatcagtctg ttaatcataa ataagaccac gggccacgga
     7981 ggctttcaat gttgagtatt tttaaaccag cgccacacaa agcgcgctta cctgctgcgg
     8041 agatcgatcc gacttatcgt cgattgcgct ggcaaatttt cctggggata ttctttggct
     8101 atgcggctta ctatttggtt cgtaagaact ttgcgcttgc tatgccttat ctggtcgagc
     8161 agggattctc tcgcggtgat ttaggtttcg cgctttcggg gatctcgatc gcttatggat
     8221 tttcgaaatt cataatgggt tcggtatcgg atcgctcgaa tccgcgcgtt ttcctgcccg
     8281 caggtttgat tctggcggcg gcagtgatgt tgtttatggg ctttgtgcca tgggcgacgt
     8341 cgagcattgc ggtgatgttt gtactgttgt tcctctgcgg ttggttccag gggatggggt
     8401 ggccgccgtg tggtcgtact atggtgcact ggtggtcgca gaaagaacgt ggcggcattg
     8461 tgtcagtgtg gaactgtgcg cacaacgtcg gtggtggtat tccgccgctg ctgttcctgc
     8521 tggggatggc ctggttcaat gactggcatg cggcgctcta tatgcctgct ttctgcgcca
     8581 ttctggtggc attattcgcc tttgcgatga tgcgcgatac cccgcaatcc tgtggcttgc
     8641 cgccgatcga agagtacaaa aatgattatc cggacgacta taacgaaaaa gcggaacagg
     8701 agctgaccgc gaagcaaatc ttcatgcagt acgtgctgcc gaacaaactg ctgtggtata
     8761 tcgccatcgc caacgtgttc gtttatctgc tgcgttacgg catcctcgac tggtcaccga
     8821 cttatctgaa agaggttaag catttcgcgc tggataaatc ctcctgggcc tacttccttt
     8881 atgaatatgc aggtattccg ggcactctgc tgtgcggctg gatgtcggat aaagtcttcc
     8941 gtggcaaccg tggggcaacc ggcgttttct ttatgacact ggtgaccatc gcgactatcg
     9001 tttactggat gaacccggca ggtaacccaa ccgtcgatat gatttgtatg attgttatcg
     9061 gcttcctgat ctacggtcct gtgatgctga tcggtctgca tgcgctggaa ctggcaccga
     9121 aaaaagcggc aggtacggca gcgggcttta ccgggctgtt tggttacctg ggcggttcgg
     9181 tggcggcgag cgcgattgtt ggctacaccg tggacttctt cggctgggat ggcggcttta
     9241 tggtaatgat tggcggcagc attctggcgg ttatcttgtt gattgttgtg atgattggcg
     9301 aaaaacgtcg ccatgaacaa ttactgcaaa aacgcaacgg aggctaatgg catgaaattg
     9361 acgctgaaaa accttagcat ggcgatcatg atgagcacta tagtcatggg aagcagtgca
     9421 atggcggcgg acagcaacga aaaaatagtc atcgcccatc gcggtgccag tggatatttg
     9481 ccggagcata cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa
     9541 caggatttgg tgatgaccaa agacgaccat ctggttgttc tgcatgacca ttatctcgat
     9601 cgtgttactg atgttgccga tcgtttcccg gaccgggcgc gcaaagacgg tcgttactac
     9661 gcgatagatt tcacgctgga tgaaattaag tcgctgaaat ttaccgaagg tttcgatatt
     9721 gaaaacggta aaaaagtgca gacttatccg gggcgtttcc caatgggtaa gtccgacttc
     9781 cgggtgcaca cctttgaaga agagattgaa tttgttcagg ggttaaatca ctctaccggg
     9841 aaaaatatcg gtatttatcc tgaaatcaaa gcgccgtggt tccatcatca ggaagggaag
     9901 gatattgcgg caaaaacgct ggaagtgctg aagaaatatg gttacaccgg taaggacgac
     9961 aaagtttatt tgcaatgttt tgatgctgat gagctgaagc gtattaagaa tgagctggaa
    10021 cctaaaatgg gcatggatct caatctggta cagttgattg cctataccga ctggaatgaa
    10081 acgcagcaga aacagccgga tggaagctgg gttaattaca actacgactg gatgtttaag
    10141 ccgggtgcta tgaaacaggt ggcggaatat gccgacggaa tcgggccgga ttaccatatg
    10201 ttgattgagg aaacatcgca gccgggtaat atcaaactca ctggcatggt gcaagatgct
    10261 cagcagaata aactggtagt gcatccttat accgtgcggt cagataaact gcctgaatac
    10321 actactgatg tgaatcagtt atatgatgct ctgtataaca aagcgggtgt aaatgggctg
    10381 tttactgatt tccctgataa ggcagtaaaa ttccttaata aagagtaatg ctactgcagg
    10441 atggcgttca tgcgccatcc tgtctcacat caaatttttc cattcaccac gagttgttcc
    10501 gtacacgttt gtagtaaaaa cagtggactg ccttctaccg gcttttcttt agccaacaac
    10561 gaaatctgtc gatagaaggt tggttcgtga ggaacggaac acaattcacc tgcaatgctt
    10621 ttcagcgtta actctggttg taaggcgatg cctaatccct gtcgaataaa cttcatcgct
    10681 gtatccgact gattaaattc acattttatc tactgggcgc actattagtg gtagtggctg
    10741 gtggaatcta tgcgctgcaa atgaagggta aaagcggtgt cacgctagta gtggcaaaag
    10801 aaattcatta atacgacacg tttcattaag attttcctca ggtaaccacc tatagtcatt
    10861 gtcgttaata aaaatgacag gcgatagggt atggcagttt cagcaaagta cgacgaattt
    10921 aatcactggt gggcaacaga gggcgactgg gttgaagagc ctaactatcg ccgtaatggt
    10981 atgagcgggg tgcaatgcgt cgagcgcaac ggcaaaaagc tgtatgtaaa gcgcatgacg
    11041 catcatctgt ttcattccgt acgttatccg ttcggccgac caacgattgt ccgtgaggtt
    11101 gcggtaatta aagaactgga acgggccggt gtcatcgtgc cgaagatcgt ttttggtgaa
    11161 gcggtgaaaa ttgagggtga atggcgagcg ttgctggtga ctgaagatat ggcggggttc
    11221 atcagcattg ctgactggta tgcccagcat gcagtatcgc cttattctga cgaagtacgg
    11281 caagccatgt tgaaagcagt ggcgctggcg tttaagaaaa tgcatagcat taatcgtcag
    11341 catggctgtt gttatgttcg ccatatctat gtgaaaacag aaggtaatgc agaagctggt
    11401 tttcttgacc tggagaaaag tcgtcgccgc ttgcgtcgcg ataaagcgat taatcatgac
    11461 ttccgccagc tggaaaaata tctggagcca atccccaaag cggactggga gcaggtgaaa
    11521 gcgtattact acgccatgta atcgttgtag gccagataag acgcataagc gttgtatctg
    11581 gcactacaac caattcacat ctcgatttca atatcgcctt ttgcccggca acaacagggc
    11641 aaaatttccc ccggctgaat aaaggctaac ggttcggcaa tccagtcaac ctgacctgca
    11701 accagacggg tgcgacagga gccgcagtaa ccttcgcgac actgatactc aaccgccaca
    11761 ttgtgggatt ccagcgccgc cagaagggaa gggtgttcat cctggcacag cagttgtgtg
    11821 ccagtgatgc gcagggtaac gcgggccatc agagttggaa gttactcaaa tcgtcggtgt
    11881 ccacttccgc gtcaatctgc ccgaccagat aagaactgac ttccacttcc tgcggagcaa
    11941 cctgcacgtt atcagacacc agccaggtgt tgatccatgg gatcgggttg gagcgcgtct
    12001 ggaacggcag atccaaaccg actgcctgca tacggatatt ggtgatgtat tcaacgtact
    12061 ggcagagaat gtctttattc agaccaatca tcgaaccgtc gcggaacaga taatccgccc
    12121 agtctttctc ctgttgagct gcctgaacaa acaggtcata gcactcctgc ttacactctt
    12181 cggcaatttc cgccatctca ggatcgtccg cgccgctgcg cagcagattc agcatatgct
    12241 gggtgccggt caggtgcagg gcttcgtcgc gggcaatcag gcgaataatt ttggcgttgc
    12301 cttccatcaa ttcgcgttct gcaaatgcga aggaacaagc aaagctgacg tagaaacgaa
    12361 tcgcttccag cgcgttaacg ctcatcaggc agagatacag ttttttcttc agctcgcgca
    12421 ggctaacggt cacagtttta ccgttaacgg tgtgggtacc ttcgcccagc agatgccagt
    12481 agctggtcat ttcgatcagc tcatcgtaat agctggagat cccttccgca cgtttctgga
    12541 tctgctcgtt ggtgacgata tcgtcaaaca caacagacgg atcgttaacg atattacgaa
    12601 tgatatgagt ataggaacgg gaatgaatcg tttctgagaa cgcccaggtt tcgacccagg
    12661 tctccagttc cgggatcgag atcagcggca gcagtgccac gttcgggcta cggccctgaa
    12721 tggaatccag cagcgtctga tatttcaggt tgctgataaa gatgtgtttt tcgtgttcag
    12781 gcagtgcctg gtaatcgata cggtcgcgag aaacgtcaac ttcttccgga cgccagaaga
    12841 aagagagctg cttttcgatc agcttttcga agatgtcata tttttgctga tcgtagcgag
    12901 ccacgttgac cggctgacca aagaacatcg gttctttgag ctgatcattt ttcgtctgtg
    12961 aaaaggtggt atatgccatg agtgtgtcct gtggttttat tacccctcac cctaaccctc
    13021 tcccgcaagg ggagagggga cggtttggtg caaaggctgg gggattatca gatcttacat
    13081 gcgccgcttt cgcagccatc gtcctggatt gacggtacca gatcgtcctg cgcgtcttca
    13141 gcgccgtcac gggtgttctg ataatacagc gttttgaccc cgaatttgta ggcggtgagc
    13201 aggtctttca gcaactgctg catcggcact tttcctgacg ggaaacgtga cggatcgtag
    13261 ttggtgttag cagagatcga ctgatcgata aatttctgca tgatacctac cagttgcagg
    13321 tagccatcgt tacccggcat ttcccacagc agctcatagg cgtcgtgcag gtgctcgtag
    13381 tccggcacca cctggcgcaa aataccgtct ttcgacgctt tgatgctgac gtaaccgcgc
    13441 ggcggttcaa taccgttagt ggcgttagag atctgtgaag aagtctctga cggcatcagg
    13501 gcagaaagcg tggagttacg caggccgtgc gttttgattg attcacgcaa tgcttcccag
    13561 tcgtaatgca gcggctcatt agcgatggta tccagatctt tcttataggt atcgatcggc
    13621 aggatccctt tcgcgtaagt ggtttcgtta aaccacgggc acgcgccttg ctcttttgcc
    13681 agctcgttag acgctttcag caggtaatac tgaatcgctt cgaaggtttt atgcgtcagg
    13741 ttgttggcgc tgccgtcgga gtaacgcaca ccgtgcttcg ccaggtagta agcgaagttg
    13801 atcacaccaa tacccagcgc acgacgaccc atcgctccac gtttggcggc cgggatcggg
    13861 taatcctgat aatccagcag cgcgtcaagt gcacgaaccg ccagaattgc cagctcttcc
    13921 agttcatcca ggctattaat tgcgcccagg ttgaaagcag acagcgtaca cagcgcgatt
    13981 tcaccgttct cgtcgttgac gtcgttcagc ggtttggtcg gcagggctat ctccaggcac
    14041 aggttagact gacgcactgg cgcgatggcc ggatcaaacg ggctatgggt attgcagtgg
    14101 tcaacgttct gaatatagat acgaccggta gacgcacgtt cctgcatcat cagcgagaac
    14161 agctcaacgg ctttcacacg ctgcttgcgg atgctgtcgt ctttctcata tttggtatac
    14221 aggcgttcaa actcttcctg atcggcgaag aatgcgtcgt acagccccgg tacgtcggac
    14281 gggctgaaca gggtgatatc ttcacctttc agcaggcggg tatacatcag tttgttgatc
    14341 tgtaccccgt agtccatatg gcgcacgcgg ttgccttcca caccacggtt gtttttcaac
    14401 accagcaggc tttccacttc cagatgccac atcgggtaga acagcgttgc cgcaccgccg
    14461 cgcacgccgc cctgagagca ggatttcacc gctgtctgga aatgtttgta gaacggaata
    14521 cagccggtat ggaacgcttc accaccgcga atcgggctac ccagcgcacg aatacgcccg
    14581 gcgttgatgc cgatcccggc acgctgggaa acgtatttca caatcgcgct ggaggtagcg
    14641 ttgatggaat ccagactgtc accgcactcg atcagtacgc aggagctgaa ctgacgagtc
    14701 ggggtacgca cgccggacat gattggcgtc ggcagcgaaa ttttaaatgt ggaaaccgcg
    14761 tcgtaaaaac gcttcacata ttgcaggcgc gtttcacgcg ggtagttcga gaacaggcac
    14821 gcggcaacca gaatataaag gaactgggcg ctttcgtaga tttcgccggt cacgcggttc
    14881 tgtaccagat atttgccttc cagctgctta acggcagcat aagagaaggt catatcacgg
    14941 tcgtgatcga taaacgtgtc catctgcttg aactcttctt ccgtgtagtc ttccagcaga
    15001 tgattatcgt atttgcccat ctcgaccatt ttcaccacgt ggtcgtacag cgcaggcggc
    15061 tcaaactggc cgtaggcttt tttacgcagg tggaagatcg ccaggcgcgc ggcgagatac
    15121 tgataatccg gcgcatcacg ggagatcagg tctgcggccg ccttgataat ggtttcgtgg
    15181 atgtcagagg tcttgatacc gtcataaaac tgaatgtggg agcgcagctc gacctgggaa
    15241 atcgaaacgt tatgcagtcc ttctgccgcc caatccagaa cgcgatggat tttgtcgaga
    15301 ttgatgcgct ctgtgctacc gtcgcgcttt gtcaccagca gattctgatt catgtatgtc
    15361 gtacctgttt ttggaattct ttccgcaata gtttcatgca gactacctgt agtacgggaa
    15421 gctgcttaga acactatata tagggggtat gtttgggatt cactgcaaga tagtgtgaaa
    15481 atggccctct tgcaagtgca taactttgtg gataactcag gaaggaaaaa gtggctttcg
    15541 cgcaccttag gtcagacaag gtgtccggga aagtcaatgg gaagagaaaa atttgttaaa
    15601 aataactgtt cggcgatttc ttgaacggca gaataggttg gataaattga tatagatggt
    15661 ttattaatct ttttgctaat gattgcaaaa attgatctaa gcacgagtca tcacataatc
    15721 tgatgtatca gttgtggtca atatataagc gaatgtaagc gattgttgat gtcgaaatgg
    15781 ttgttttcta ttcggttatt gttaaatgcg aagcca
//
LOCUS       DAZFKM010000112        15801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFKM010000112 DAZFKM010000000
VERSION     DAZFKM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(2..1264)
                     /locus_tag="ACHKAY_003721"
     CDS             complement(2..1264)
                     /locus_tag="ACHKAY_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5702321.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
     gene            complement(1428..1512)
                     /locus_tag="ACHKAY_003722"
     tRNA            complement(1428..1512)
                     /locus_tag="ACHKAY_003722"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1476..1478),aa:Leu,seq:caa)
     gene            1708..2727
                     /gene="ahr"
                     /locus_tag="ACHKAY_003723"
     CDS             1708..2727
                     /gene="ahr"
                     /locus_tag="ACHKAY_003723"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO5702322.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2857..4359
                     /gene="yjgR"
                     /locus_tag="ACHKAY_003724"
     CDS             2857..4359
                     /gene="yjgR"
                     /locus_tag="ACHKAY_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO5702323.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4478..5560)
                     /gene="lptG"
                     /locus_tag="ACHKAY_003725"
     CDS             complement(4478..5560)
                     /gene="lptG"
                     /locus_tag="ACHKAY_003725"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5702324.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5560..6660)
                     /gene="lptF"
                     /locus_tag="ACHKAY_003726"
     CDS             complement(5560..6660)
                     /gene="lptF"
                     /locus_tag="ACHKAY_003726"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5702325.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6723..6773)
                     /gene="ytgA"
                     /locus_tag="ACHKAY_003727"
     CDS             complement(6723..6773)
                     /gene="ytgA"
                     /locus_tag="ACHKAY_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO5702326.1"
                     /translation="MSIIILATISTTQENK"
     gene            6927..8438
                     /gene="pepA"
                     /locus_tag="ACHKAY_003728"
     CDS             6927..8438
                     /gene="pepA"
                     /locus_tag="ACHKAY_003728"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO5702327.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8792..9235
                     /gene="holC"
                     /locus_tag="ACHKAY_003729"
     CDS             8792..9235
                     /gene="holC"
                     /locus_tag="ACHKAY_003729"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO5702328.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9235..12090
                     /gene="valS"
                     /locus_tag="ACHKAY_003730"
     CDS             9235..12090
                     /gene="valS"
                     /locus_tag="ACHKAY_003730"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO5702329.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12144..13340)
                     /gene="yjgN"
                     /locus_tag="ACHKAY_003731"
                     /pseudo
     CDS             complement(12144..13340)
                     /gene="yjgN"
                     /locus_tag="ACHKAY_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            13533..14036
                     /gene="yjgM"
                     /locus_tag="ACHKAY_003732"
     CDS             13533..14036
                     /gene="yjgM"
                     /locus_tag="ACHKAY_003732"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO5702330.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(14082..14498)
                     /gene="rraB"
                     /locus_tag="ACHKAY_003733"
     CDS             complement(14082..14498)
                     /gene="rraB"
                     /locus_tag="ACHKAY_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO5702331.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14660..15673
                     /gene="argF"
                     /locus_tag="ACHKAY_003734"
     CDS             14660..15673
                     /gene="argF"
                     /locus_tag="ACHKAY_003734"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO5702332.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         4042 a         4002 c         4099 g         3658 t
ORIGIN      
        1 attatttccc catgaattta gcaaattcat aaggtgtaac agagttattt cgattggcct
       61 caagaaaatc agcccaccat tgaaccatta atctacgttc gtctaaatgc tcagaggtat
      121 gaatatatgc tgcacgcaca ttgttacgct ctgtatgact caactgccgt tctatcgcgt
      181 catcgctcca taagcctgat tcaccaagcg cgccacgcgc catcgtccta aatccatgcc
      241 cacagacctc agttttcgtg tcatacccca tcgcacgcaa ggcgctattt actgtgttct
      301 cactcataac tttggccgcg tcgtgatccc ctggaaaaag taactcttta tcgccactaa
      361 tctgcttgag ttgctctaac aaaaccatcg cctgtcgact aagaggaacg atatgctcct
      421 ctttcatctt catgccacga tacgagtaac gcacgccttt aatttcttct cgctttgcag
      481 gtatacgcca aagagattta tcgaagtcga actcatccca tcgcgcgaaa cgtaactcac
      541 tggaacgtac aaaagttagt aaggaaagct cgaccgcgat ccttgtcatt acacgaccac
      601 gatatgcagc aagacgtgca agaaactcag agaagcggct agagggtaaa gctggatagt
      661 gtcgtgcttt ggttgtagat aacgcaccgg ccatgtcact ggctggatta gagtcgatgt
      721 aatcgttctg tacggcataa cgcataatgg ccgtgacgcg ctgttgcagg cgctgagcga
      781 catcgtgttt gccactggca tcaacttttt taatcggggc taacaggtgg ctggttttaa
      841 gctgccgaat gtcggacgaa ccgatatgag gaaaaatata aagctcaaga tagcgcagaa
      901 tgcgtgatcg atggtcttca ctccagcgct tattactggc atgccattca cgagcaattg
      961 tttcgaaagt gtatgcgccc gaattctcag cctgagcttc tttctgctcg gcttttgggt
     1021 caatgccctg cactaagagc tttttagcct cgtcacgctt tgctcttgcc tgagcaagtg
     1081 tcaccatggg ccaaacacca aaagcgaggc gatcctcttt tttgtcagag gggcgtctgt
     1141 atttcatgcg ccagtattta gaacccttag ccgaaacctc gagatacaaa ccgccgccat
     1201 cggccatttt gtaggttttc tcttttggct ttgctgtctc gacctgtctg gcgttgagct
     1261 tcatttgggg gcacatttct aatcgaagtt aagatgcccc caattatgcc cccaatagca
     1321 tccggatttc aacggactag ctcggacagc tcaggacaga aaaaccgcta caggcattga
     1381 ttttaaaggg tttgctggat tttctcggat ggtcttggaa gtactaatgg tgccgaaggc
     1441 cggactcgaa ccggcacgta tttctacggt tgattttgaa tcaactgcgt ctaccgattt
     1501 cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc
     1561 accatgcaag cgccagcttg cacccacctc gctaagtgct gaaattttca gcattacact
     1621 cagcgatatg taacctttgt cacactccag gcaccccgcc ctgccatgct ctacacttcc
     1681 caaacaacac cagagaagga cccaaaaatg tcgatgataa aaagctatgc cgcaaaagaa
     1741 gcgggcggcg aactggaagt ttatgagtac gatcccggtg agctgaagcc acaagatgtt
     1801 gaagtgcagg tggattactg cgggatttgt cattccgatc tgtcgatgat cgataacgaa
     1861 tggggatttt cacaatatcc gctggttgcc gggcatgagg tgattgggcg cgtggtggca
     1921 ctcgggagcg ccgcgcagga taaaggtttg caggtcggtc agcgtgtcgg gattggctgg
     1981 acggcgcgta gctgtggtca ctgcgacacc tgtattagcg gtaatcagat caactgcgag
     2041 caaggtgcgg tgccgacgat tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac
     2101 tggcaatggg tgattccact gccagaaaat attgatatcg agtccgccgg gccgctgttg
     2161 tgcggcggta tcacggtctt taaaccactg ttgatgcacc atatcactgc taccagccgc
     2221 gttggggtaa ttggtattgg cgggctgggg catatcgcta taaaacttct gcacgcaatg
     2281 ggatgcgagg tgacggcctt tagttctaat ccggcgaaag agcaggaagt actggcgatg
     2341 ggtaccgata aagtggtgaa tagccgcgat ccgcaggcac tgaaagccct gtcggggcag
     2401 tttgatctca ttatcaatac tgtgaacgtc agcctcgact ggcagcctta ttttgaggcg
     2461 ctgacctatg gcggtaattt ccatacggtc ggtgcggttc tcacgccgct gtctgttccg
     2521 gcctttacgt taattgcggg cgaccgcagc atctctggtt ctgctaccgg cacgccttat
     2581 gagctgcgta agctgatgcg ttttgccgcc cgcagcaagg ttgcgccgac caccgaactg
     2641 ttcccgatgt cgaaaattaa cgacgccatc cagcatgtac gcgatggtaa agcgcgctac
     2701 cgcgtggtcc tgaaagccga cttctgatcc ctttgccggg agcctgtcgc ccggcactta
     2761 aatctctttg gaaggcgtct cgcaatctgc tgaatttagc ggtgaagagt gcacagccgt
     2821 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     2881 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     2941 actggcgcaa cggggacggg taaaactgtc acgctgcaaa aactggcgga gtctctatcg
     3001 gaaatcggcg tgccggtgtt tatggccgat gtgaaaggcg atctgaccgg tatcgcgcag
     3061 gcaggaacgg cgtcggaaaa actgctcgca aggcttaaaa atatcggcgt aaatgactgg
     3121 caaccgcatg ctaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     3181 cgggcgacgg tttcggatct ggggccgctg ttgctggcgc ggctgttgaa tctcaacgat
     3241 gtgcaatctg gcgtgctgaa tatcatcttc cgaattgctg acgatcaggg gctgttactg
     3301 ctcgacttta aagatctgcg ggcgattacc cagtacatcg gcgataacgc caaatctttc
     3361 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cgggctgttg
     3421 tcgctggaac agcaaggcgc agcacacttc tttggtgagc cgatgctgga tatcaaagac
     3481 ttgatgcgca ccgatgccaa cggtaaaggc gttattaata tcctcagcgc cgagaagctt
     3541 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgatttcaga gttgtatgaa
     3601 caattgccgg aagcgggcga tctggagaag ccaaaactgg tgtttttctt cgacgaagca
     3661 catctgctgt ttaacgatgc accgcaggta ctgctggata agattgagca ggtgataagg
     3721 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc tgatattccg
     3781 gataatgtgc tcggccagct cggtaatcgc gttcagcacg ctttgcgggc ttttacgcca
     3841 aaagatcaga aagcggtaaa agctgcggcg caaaccatgc gggccaatcc ggcgtttgat
     3901 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgca
     3961 aaaggaagtc cttctgtggt ggaacgggcg atggtgattg cgccttgttc gcggatggga
     4021 ccggtgacgg aagatgagcg caatggcttg attaaccact ccccggtgta tggcaagtac
     4081 gaggatgacg tggaccggga atccgcctat gagatgttgc aaaaaggcgt acaggcgagt
     4141 attgaacagc aaaataatcc tccagcgaaa gggaaagaga tagcggtaga tgacggcatt
     4201 cttggtggat tgaaggatat tttgttcggc actaccggac cacgcggcgg gaagaaagat
     4261 ggtgtggtgc agacaatggc caaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     4321 gggatgttgg ggagtttgct gggagggaga agaaggtaag tcaggcgcga tctgtccccc
     4381 tcaccctaac cctctcccca aaggggcgag gggaccgatc gaactcgtat ttgactttat
     4441 caacaatgtg aaagggagct ttcgctccct tgcttggtta cgattttctc attaacagcc
     4501 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgataatc ggcgggatgc
     4561 cgtaaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     4621 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     4681 acagcgccat cagcatcatc accgccacgg atagcggctg gaagattttg ctccacatat
     4741 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcacg tagttgtgca
     4801 aaccgctgat tgagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     4861 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     4921 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcag
     4981 tagcggcata gcgtacggat tgcagacgac gattttcgtt aaaggcataa atgctgatgc
     5041 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     5101 cccataagcc ttgctgggta gagagcaacg agccgccgta catcgcctgc gcacggtagt
     5161 tacgcgccat ctgctcgccc tgcggcgcga cccattcgcc aatcgccatc gtcagcaaga
     5221 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     5281 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     5341 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     5401 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcaactga tcgacaaatt
     5461 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     5521 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     5581 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     5641 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     5701 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
     5761 cagcatcgac agtacgcgtc cctgacgtgg gttaaccacg ctcagcggta cgaccataag
     5821 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgagcacg
     5881 atcggtgtca gtgttccaca atgtgcacat gtccatctgg tcggtatcgt tcgggtcgag
     5941 cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg aaatccgtaa tgcggaaatc
     6001 acgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
     6061 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
     6121 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
     6181 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
     6241 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accacggtcc
     6301 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat accgcaagga tcattgccgc
     6361 tttcaccaga accgctttgc tcaggccgca ggcatgcatt accgtaattt cactttcggt
     6421 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
     6481 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
     6541 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
     6601 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
     6661 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
     6721 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
     6781 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
     6841 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
     6901 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
     6961 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
     7021 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
     7081 tggaaggaaa accggggcag acattgttgc tgcaccatgt tccgaatgta ctttccgagc
     7141 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgagcgtcag tacaagcagg
     7201 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
     7261 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
     7321 cggcaaaaga aacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
     7381 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
     7441 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaag gatcttggca
     7501 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
     7561 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
     7621 ggatgcattc ctatctggcg gtcggtcagg gttcgcagaa cgaatcgctg atgtcggtga
     7681 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
     7741 taaccttcga ctccggcggt atctcgatca agccttcaga aggcatggat gagatgaagt
     7801 acgatatgtg cggtgcggca gcggtttacg gtgtgatgcg tatggtcgcg gagctacaac
     7861 tgccgattaa cgttatcggc gtgttggcag gctgcgaaaa catgcctggc ggacgagcct
     7921 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaatactg
     7981 acgctgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgagcgt tttgagccgg
     8041 aagcggtgat tgacgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcaca
     8101 ttaccggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
     8161 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa cagctggagt
     8221 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
     8281 gcttcctgtc gcgctttacc cgtaagtaca actgggcgca cctggatatc gccggaaccg
     8341 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
     8401 tcctgttgaa ccgcgctggg tttaacggcg aagagtaatt gcgtcaggca aggctgttat
     8461 tgccggatgc ggcgtgaacg ccttatccga cctacacagc actgaactcg taggcctgat
     8521 aagacgcgca agcgtcgcat caggcaatgc tgttattgtc ggatgcggca tgaacgcctt
     8581 atccgaccta cacagcaccg aactcgtagg cctgataaga cacaacagcg tcgcatcagg
     8641 caatgctgtt attgtcggat gcggcgtgag cgccttatcc gacctacaca cagcactgaa
     8701 ctcgtaggcc tgataagaca caacagcgtc gcatcaggcg ctgcggtgta tacctgatgc
     8761 gtatttaaat ccaccacaag aagccccatt tatgaaaaac gcgacgttct accttctgga
     8821 caatgacacc accgtcgatg gcttaagcgc cgttgagcaa ctggtgtgtg aaattgccgc
     8881 agaacgttgg cgcagcggta agcgcgtgct catcgcctgt gaagatgaaa agcaggctta
     8941 ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc
     9001 gggagaagga ccgcgtggcg gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag
     9061 cagcccgcgc gatatattga ttagtctgcg aacatgcttt gcagattttg ccaccgcttt
     9121 cacagaagtg gtagacttcg ttccttatga agattctctg aaacaactgg cgcgcgaacg
     9181 ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg gcaacctgga aataatggaa
     9241 aagacatata acccacaaga tatcgaacag ccgctttacg agcactggga aaagcagggc
     9301 tactttaagc ctaatggcga tgaaagccag gaaagtttct gcatcatgat cccgccgccg
     9361 aacgtcaccg gcagtttgca tatgggtcac gccttccagc aaaccatcat ggataccatg
     9421 atccgctatc agcgcatgca gggcaaaaac accctgtggc aggtcggtac tgaccacgcc
     9481 gggatcgcta cccagatggt cgttgagcgc aagattgccg cagaagaagg taaaacccgt
     9541 cacgactacg gccgcgaagc tttcatcgac aaaatctggg aatggaaagc ggaatccggc
     9601 ggcacgatta cccgtcagat gcgccgtctc ggcaactccg tcgactggga gcgtgaacgc
     9661 ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa
     9721 gaagacctga tttaccgtgg caaacgcctg gtaaactggg acccgaaact gcgcaccgct
     9781 atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat
     9841 ccgctggctg acggtgcgaa aaccgcagac ggtaaagatt acctggtggt tgcgactacc
     9901 cgtccggaaa ccctgctggg cgataccggt gtagccgtta acccggaaga tccgcgttac
     9961 aaagacctga ttggcaaata tgtcattcta ccgctggtta accgtcgtat tccgatcgtt
    10021 ggcgacgaac acgccgacat ggaaaaaggc accggctgcg tgaagatcac cccggcgcac
    10081 gactttaacg actatgaagt cggtaaacgt cacgccctgc cgatgatcaa catcctgacc
    10141 tttgacggcg atatccgtga aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac
    10201 gtttattcca gcgagatccc ggcagaattc cagaaactgg agcgttttgc cgcacgtaaa
    10261 gcagtcgttg ctgcggttga cgcactcggc ctgctggaag aaattaaacc gcacgacctg
    10321 accgttcctt acggcgaccg tggcggcgta gttatcgaac caatgctgac cgaccagtgg
    10381 tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag cggttgagaa cggcgacatt
    10441 cagttcgtac cgaagcagta cgaaaacatg tatttctcct ggatgcgcga tattcaggac
    10501 tggtgtatct ctcgtcagct gtggtggggt caccgtatcc cggcatggta tgacgaagcg
    10561 ggtaatgttt atgttggccg caacgaagaa gaagtgcgca aggaaaataa cctcggtgcc
    10621 gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct ggttctcttc tgcgctgtgg
    10681 accttctcta cccttggctg gccggaaaac accgacgccc tgcgtcagtt ccacccaacc
    10741 agcgtgatgg tatccggctt cgacatcatc ttcttctgga ttgcccgcat gatcatgatg
    10801 accatgcact tcatcaaaga tgaaaatggc aaaccgcagg tgccgttcca cactgtttac
    10861 atgaccggtc tgattcgtga cgacgaaggc cagaaaatgt ccaaatccaa gggtaacgtt
    10921 atcgacccgc tggatatggt tgacggtatt tcgctgccag aactgctgga aaaacgtacc
    10981 ggcaatatga tgcagccgca gctggcggac aaaatccgta agcgcaccga gaaacagttc
    11041 ccgaacggca ttgagccgca cggcaccgat gccctgcgct tcaccctggc ggcgctggcg
    11101 tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg aaggttaccg taacttctgt
    11161 aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc
    11221 tttaacggcg gcgaaatgac actgtcgctg gcggaccgct ggattctggc agagttcaac
    11281 cagaccatca aagcgtaccg cgaagcgctg gacagcttcc gcttcgatat cgctgcaggc
    11341 attctgtatg agttcacctg gaaccagttc tgtgactggt atctggagct gaccaagccg
    11401 gtaatgaacg gtggcaccga agccgaactg cgcggtactc gccatacgct ggtgactgta
    11461 ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt tcatcaccga aaccatctgg
    11521 cagcgtgtga aagtactttg cggtatcact gccgacacca tcatgctgca gccgttcccg
    11581 cagtacgatg catctcaggt tgatgaagcc gcactggccg acaccgaatg gctgaaacag
    11641 gcgatcgttg cggtacgtaa catccgtgca gaaatgaaca tcgcgccggg caaaccgctg
    11701 gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc
    11761 ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt
    11821 ccggtttccg ttacgaagat catcgacggc gcagagctgc tgatcccgat ggctggcctc
    11881 atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag tggcgaagat cgaaggcgaa
    11941 atcagccgta tcgagaacaa gctggcgaat gaaggctttg tcgcccgcgc accggaagcg
    12001 gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg aagcgaaagc gaagctgatt
    12061 gaacagcagg ctgttatcgc cgcgctgtaa tcaacaccgt gatgaaaacg aaggccggag
    12121 aatgctccgg ccttttttat ctcttacaga aatggtaaag aaggcataat tccccgtgaa
    12181 atactggcta agaagccttt atctggttgt tcttctttat tgattaaagg aaggtcatcc
    12241 aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat tttcagcagt
    12301 ggataagcca gaccgcccgt aatcccggat atcactacca acgcacacat gcgataaagc
    12361 ataccgtggt aagttaaagt tgagcgaaaa cgaatacgcc catcattcag tgacaggttg
    12421 ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc aatcccaaaa
    12481 taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg ctgtaaattc
    12541 tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg atcgaagata
    12601 atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgaggat ggcatatttt
    12661 atacaatacg cagtatctaa tttcaccttg aaacgatgta taccgaaact ggtattgctc
    12721 cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat ggaaacaata
    12781 ccaactattg ccatcagaac cacagatata ataacactac ttgaactatt ggcatgtagc
    12841 atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat cggcaagaaa
    12901 aaggttactc accagaaccc tttcatagag caattaaaac taaatcttac gccgttgaga
    12961 ctagtcatca aggcctgata acgcagtcct tttgctgcca taaatataag caaaaccaac
    13021 agtgacaaag tcaaaacaca gccaataagc ggcatatctg ctgacactgt cattaagatt
    13081 gcgaaataga aaaaaacaaa aacaagacaa ctaacaaaaa cattcccacc ggtaatccca
    13141 taagaaaatc gttgtccgtt aacctccata ttagcataga gataacgctt acatttcatt
    13201 aatgcccatg gtaaatagat acctagcgtt ataatcgtta acaacacatt caccacacaa
    13261 ataagaaaat atctctcacc tgtatcatga aaaacaaacg aatgggacgg aacatccatt
    13321 tcattaataa cttgagccat aaaaattccc tgaattaaaa aatccattga tataagttat
    13381 agatacagac agtaaaatgt tctgccacgt tacaatactc ttatcagtaa taggctggca
    13441 aaaactttat tgctttataa gatgcttgcg cacgtaagaa gagagtggta ttaaacaaat
    13501 gtataattac tttattttta ttgggttaaa ctatgaataa cattgcgccg caatcacctg
    13561 taatgcgtcg cctgacgctg caggataatc ctgctattgc ccgcgtcatt cgtcaggtat
    13621 ccgccgaata cggtcttacc gctgataaag gctacaccgt cgccgatccg aatcttgacg
    13681 agctgtatca agtgtatagt cagccgggtc atgcatattg ggtggttgag tacgagggtg
    13741 aagtggtcgg cggcggtggg attgcgccat tagcaggtag tgaatcggac atttgcgaac
    13801 tgcaaaagat gtattttctt ccggctattc gcggtaaagg gctggcgaaa aaactggcct
    13861 taatggcgat ggagcaggca cgagagatgg gcttcaaacg ctgctatctg gaaacgaccg
    13921 cttttttaaa ggaagccatt gcgctttatg agcatttggg ctttgagcat atcgactatg
    13981 cgcttggctg cacgggccat gtcgattgtg aagtgcggat gctacgcgaa ctctaaaaaa
    14041 atgcccgcta gtgtgtgcta tcgggcattt taatcaatta attagtggcg aaccccgtca
    14101 tcgtcttcat cgacaaaatc ttcatcgtcg ccgtcttcgc cgttgggatc ttcaaagtaa
    14161 gtgccccaac cgtcgtactc aacgtcaaat ttctccgcca gcgtcatcag ttgttcaacc
    14221 tgggcatcga tcagatcggc attcaacgcg cactcgctga ggatgtcgca gcaaatcacg
    14281 atatcaccat cttcaacttc cagctcttct ggatcggtca cttcgtaacc gagtttaaac
    14341 gcttcaactg ctgctttttc cagggtttcc agatcgtctg cggaaagatg gtgttcgatg
    14401 gtatacagcg cgtccggatc gctgccatct tccagtaatt cttcaataat caaacgtgtt
    14461 tcttcacgct gttcttccag ttgttccggg tttgccatgg ctcattcctc ttaatgtcct
    14521 gccgatactc ttattgtcac acaccactga cattgcctcc acctttgtga caaagattta
    14581 tgctttagac ttgcaaatga ataatcatcc atataaattg aattttaatt cattgaggtg
    14641 ttagccacag gagggatcta tgtccgggtt ttatcataag catttcctga aattactcga
    14701 tttcacgcca gctgaactca acagcctgct gcagttagcc gcgaagctga aagccgataa
    14761 gaaaagcggc aaagaagaag ccaaactcac tggtaaaaac atcgcgctca tcttcgaaaa
    14821 agactcgacc cgtacccgat gctctttcga agttgccgca tatgaccagg gtgctcacgt
    14881 tacttatctc ggcccaagcg gcagtcagat tggtcataaa gagtcgatta aagacactgc
    14941 ccgcgtgctg ggtcgcatgt atgacggtat tcagtatcgc ggctatggtc aggagattgt
    15001 cgaaacactg gcggaatacg ctggcgtgcc ggtatggaac ggcctgacca atgagtttca
    15061 ccccacgcag ttgctggcgg atctcctcac catgcaggag catttacccg gtaaagcatt
    15121 caacgaaatg acgctggtct atgcaggtga cgcacgtaac aacatgggca attcgatgct
    15181 cgaagctgcg gcgcttaccg gtctggattt gcgtctggtc gcgccacagg catgctggcc
    15241 ggaagctgcg ctggttacgg aatgccgcgc tctggcacag caaaatggcg ggaatattac
    15301 gctgacggaa gatgtagcta aaggagttga aggtgctgac tttatctata ccgatgtatg
    15361 ggtgtcgatg ggtgaagcaa aagagaaatg ggctgaacgc attgcattgc tgcgtgatta
    15421 tcaagtgaac agcaagatga tgcagttgac cggtaacccg gaggtcaaat tcctccactg
    15481 cctgcccgcg tttcatgacg accaaacgac gcttggcaag aaaatggcgg aagagtttgg
    15541 cctacatggc ggaatggaag tgactgatga ggtcttcgaa tctgccgcca gcattgtatt
    15601 tgatcaggcg gaaaaccgca tgcatactat caaagcggtg atggtcgcga cgctcagtaa
    15661 attaaataat taatatctct atcaagatgc cgggaaatac aaaaacatcc cggcatcaca
    15721 ttattttatg cagtgatagt ttttttcttc cagccaaatt tttctattgc tatacgtaaa
    15781 ttttccggag gatttaattt t
//
LOCUS       DAZFKM010000113        15708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFKM010000113 DAZFKM010000000
VERSION     DAZFKM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            14..895
                     /gene="yieE"
                     /locus_tag="ACHKAY_003735"
     CDS             14..895
                     /gene="yieE"
                     /locus_tag="ACHKAY_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO5702333.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1100..2569
                     /gene="lysP"
                     /locus_tag="ACHKAY_003736"
     CDS             1100..2569
                     /gene="lysP"
                     /locus_tag="ACHKAY_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO5702334.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2862..4853
                     /gene="cirA"
                     /locus_tag="ACHKAY_003737"
     CDS             2862..4853
                     /gene="cirA"
                     /locus_tag="ACHKAY_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO5702335.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSLITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4885..5721)
                     /gene="yeiG"
                     /locus_tag="ACHKAY_003738"
     CDS             complement(4885..5721)
                     /gene="yeiG"
                     /locus_tag="ACHKAY_003738"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO5702336.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5979..6647
                     /gene="folE"
                     /locus_tag="ACHKAY_003739"
     CDS             5979..6647
                     /gene="folE"
                     /locus_tag="ACHKAY_003739"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO5702337.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6664..7821
                     /gene="yeiB"
                     /locus_tag="ACHKAY_003740"
     CDS             6664..7821
                     /gene="yeiB"
                     /locus_tag="ACHKAY_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO5702338.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLCYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7963..9003
                     /gene="galS"
                     /locus_tag="ACHKAY_003741"
     CDS             7963..9003
                     /gene="galS"
                     /locus_tag="ACHKAY_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO5702339.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            9283..10281
                     /gene="mglB"
                     /locus_tag="ACHKAY_003742"
     CDS             9283..10281
                     /gene="mglB"
                     /locus_tag="ACHKAY_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO5702340.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10342..11862
                     /gene="mglA"
                     /locus_tag="ACHKAY_003743"
     CDS             10342..11862
                     /gene="mglA"
                     /locus_tag="ACHKAY_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO5702341.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11878..12888
                     /gene="mglC"
                     /locus_tag="ACHKAY_003744"
     CDS             11878..12888
                     /gene="mglC"
                     /locus_tag="ACHKAY_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO5702342.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12959..14194)
                     /gene="preA"
                     /locus_tag="ACHKAY_003745"
     CDS             complement(12959..14194)
                     /gene="preA"
                     /locus_tag="ACHKAY_003745"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO5702343.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14188..15426)
                     /gene="preT"
                     /locus_tag="ACHKAY_003746"
     CDS             complement(14188..15426)
                     /gene="preT"
                     /locus_tag="ACHKAY_003746"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO5702344.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3736 a         3872 c         4114 g         3986 t
ORIGIN      
        1 gtggtaagcg actatgcaca tcaccctccg gcagttggaa gtttttgcag aagtattgaa
       61 aagtggatca accacccagg cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc
      121 agccttgacc gacctggaag ggcagcttgg cgtgcaactg tttgatcgcg tggggaaaag
      181 actggttgtt aatgaacacg ggcggctgct ctatccgcgt gcgttggcat tgcttgaaca
      241 ggcggttgaa atcgaacaac tgtttcgcga agacaacggc gcgattcgta tctatgccag
      301 tagtaccatc ggtaactaca ttctgcctgc agttatcgcc cgttatcgcc atgattatcc
      361 gcagttgccg attgaactta gcgttgggaa cagccaggac gtgatgcaag cggtgctgga
      421 tttccgcgtt gatattggct ttattgaagg accgtgccac agcactgaaa tcatttctga
      481 accgtggctg gaagacgagc tggtggtttt cgccgcgccg acttcgccgt tggctcgtgg
      541 tccggtcacc ttagaacagc tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg
      601 cacgcgggag attgtcgatt atctgttgct gtcacattta ccgaagtttg agatggcgat
      661 ggaattaggt aactccgagg caatcaaaca tgcggtgcgt catgggttgg gaattagttg
      721 cctgtcgcga cgggtgatta aagatcaatt gcaggcaggc acattaagtg aagttgcggt
      781 ccctctcccg cgcctgatgc gtacgttgtg gcgtatacat catcggcaaa aacacctttc
      841 caacgcgcta cggcgctttc tggactattg cgatcccgcg aatgtaccgc gttaagttgc
      901 tgtacaagaa catgctggtg ctgtgtcgat ttcgtgacgc agcgccctca gcatgcattc
      961 gccagaaaag agattggctg ctttacttat aatccctggg cgatcatgaa ggtgtcttat
     1021 aaccgtgtat ttctgccgga aggattgcca atcgtctgct acaatcgcgc ctcattttta
     1081 agatggatag catttttgta tggtttccga aactaaaacc acagaagcgc cgggcttacg
     1141 ccgtgaatta aaggcgcgtc acctgacgat gattgccatt ggcggttcca tcggtacagg
     1201 tctttttgtt gcctctggcg caacgatttc tcaggcaggt ccgggcgggg cattgctctc
     1261 gtatatgctg attggcctga tggtttactt cctgatgacc agtctcggtg aactagctgc
     1321 atatatgccg gtttccggtt cgtttgccac ttacggtcag aactatgttg aagaaggctt
     1381 tggcttcgcg ctgggctgga actactggta caactgggcg gtgactatcg ccgttgacct
     1441 ggttgcagct cagctggtca tgagctggtg gttcccggat acaccgggct ggatctggag
     1501 tgcgttgttc ctcggcgtta tcttcctgct gaactacatc tcagttcgtg gctttggtga
     1561 agcggaatac tggttctcac tgatcaaagt cacgacagtt attgtcttta tcatcgttgg
     1621 cgtgctgatg attatcggta tcttcaaagg cgcgcagcct gcgggctgga gcaactggac
     1681 aatcggcgaa gcgccgtttg ctggtggttt tgcggcgatg atcggcgtag ctatgattgt
     1741 cggcttctct ttccagggaa ccgagctgat cggtattgct gcaggcgagt ccgaagatcc
     1801 ggcgaaaaac attccacgcg cggtacgtca ggtgttctgg cgaatcctgt tgttctatgt
     1861 gttcgcgatc ctgattatca gcctgattat tccgtacacc gatccgagcc tgctgcgtaa
     1921 cgatgttaaa gacatcagcg ttagtccgtt caccctggtg ttccagcacg cgggtctgct
     1981 ctctgcggcg gcggtgatga acgcagttat tctgacggcg gtgctgtcag cgggtaactc
     2041 cggtatgtat gcgtctactc gtatgctgta caccctggcg tgtgacggta aagcgccgcg
     2101 cattttcgct aaactgtcgc gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt
     2161 gattgccggt ctgtgcttcc tgacctccat gtttggcaac cagacggtat acctgtggct
     2221 gctgaacacc tccgggatga cgggttttat cgcctggctg gggattgcca ttagccacta
     2281 tcgcttccgt cgcggttacg tattgcaggg acacgacatt aacgatctgc cgtaccgttc
     2341 aggtttcttc ccactggggc cgatcttcgc attcattctg tgtctgatta tcactttggg
     2401 ccagaactac gaagcgttcc tgaaagatac cattgactgg ggcggcgtag cggcaacgta
     2461 tattggtatc ccgctgttcc tgattatttg gttcggctac aagctgatta aaggaactca
     2521 cttcgtacgc tacagcgaaa tgaagttccc gcagaacgat aagaaataag tttcctccct
     2581 tccttgctaa gccctctcaa ccgagagggc tttttcaatt ccatttccct gacaaatcat
     2641 gcggatataa aatttaacat ttggattgat aattgttatc gtttgcatta tcgttacgcc
     2701 gcaatcaaaa aaggctgaca aatcagaggc tgttccggct ttctgggatg atcacctgca
     2761 tacaaataag tccaccgcga tgctgccgta cgcaagggga cgtgaagaag atgtgagcga
     2821 taacccattt tattttcgta gttacctcat ggagatatgg aatgtttagg ttgaaccctt
     2881 tcgtacgggt cgggctgtgt ttgtccgcta tttcttgtgc atggcctgtg ttagcggtcg
     2941 atgatgatgg cgaaacgatg gttgtcactg catcttccgt tgaacaaaac ctcaaagatg
     3001 cacccgccag tatcagcgtc attacccagg aagacctgca gcgaaaaccg gtacagaatc
     3061 tgaaggatgt cctcaaagaa gtgcctggcg tacaactgac gaacgaaggg gataaccgta
     3121 agggcgttag tattcgtggt ctggacagca gctacaccct gattctcgtc gacggtaaac
     3181 gcgttaactc ccgcaatgcc gtcttccgcc acaatgattt cgatctgaac tggatcccgg
     3241 tcgattccat cgaacgtatt gaagtggttc gtggcccgat gtcgtcgcta tacggctccg
     3301 atgcgctcgg cggtgtagtg aatatcatca ccaaaaaaat cggtcagaaa tggtcgggta
     3361 ccgttaccgt cgataccacc attcaggaac atcgcgatcg cggtgatacc tataacggtc
     3421 agttctttac cagtggacca ttaattgatg gtgtgctggg aatgaaagct tacggcagcc
     3481 tggcaaaacg tgaaaaggat gacccgcaaa actcaacgac caccgatacc ggagaaacgc
     3541 cgcgtattga aggattctcc agccgcgacg gcaatgtcga atttgcctgg acaccgaatc
     3601 aaaatcacga ttttactgcc ggatacggtt tcgaccgtca ggatcgtgat tccgactcgc
     3661 tggacaaaaa ccgcctggaa cgccagaact actccgtcag ccataatggg cgttgggatt
     3721 acggcaccag cgaactgaaa tactacggtg agaaagtcga gaacaaaaac cctggcaaca
     3781 gcagcctgat aacttccgaa agcaatacgg tcgacggcaa atacacgttg ccgctgacgg
     3841 caattaatca gtttctcacg gttggcggtg aatggcgtca cgacaaactt agcgatgcgg
     3901 tgaacctgac cgggggaacc agctccaaaa cgtctgccag ccagtacgcg ctgtttgtgg
     3961 aagatgaatg gcggatcttc gagccgctgg cgctgacgac cggcgtgcgt atggacgatc
     4021 acgaaaccta cggtgaacac tggagtccgc gtgcctacct ggtttataac gccaccgaca
     4081 ccgtaacggt gaaagggggc tgggcgacgg catttaaagc accttctctg ttgcaactta
     4141 gccctgactg gacgagcaat tcctgccgtg gcgcatgtaa gattgtgggt agcccggatc
     4201 tgaaaccaga aaccagcgaa agttgggagc tggggcttta ctacatgggt gaagaaggct
     4261 ggctggaagg ggttgaatcc agcgttaccg ttttccgtaa cgatgtgaaa gatcgtatca
     4321 gcatcagccg tacgtctgac gtcaacgctg caccgggcta ccaaaacttt gttggttttg
     4381 agacgggcgc taacggacgg cgcataccgg tatttagcta ctacaacgtt aacaaagctc
     4441 gtattcaggg cgtggaaacc gaactgaaaa ttccgttcaa cgatgaatgg aaactgtcga
     4501 tcaactacac ctacaacgat ggtcgtgatg tcagcaacgg cgaaaacaaa ccgctatccg
     4561 atctgccgtt ccatactgct aacggtacgc tggactggaa accgctggcg ctggaagact
     4621 ggtcattcta tgtttctggt cactataccg ggcagaaacg cgccgacagc gcgacggcta
     4681 aaacaccggg cggttatacc atctggaata ccggcgcggc ctggcaggtg actaaagacg
     4741 tcaaactgcg cgcaggcgtg ctgaaccttg gcgacaagga tctcagtcgt gacgactaca
     4801 gctataacga agacggacgt cgttacttta tggcagtgga ttatcgcttc tgatgagaag
     4861 atgcccggcg aaccgggcgg actttcactt cagtaaatac tgcgcatgga agcgcaggtg
     4921 atcctctata aaagaggcga tgaagtagta actgtgatca tatcccggct gaatacgcag
     4981 cgtcatcggc cacgctttct ggcgcgcggc ttctgccagt accgcaggtt gcaactggtc
     5041 ggcaagaaac tgatcattat cgccctgatc gataagcgtc gggatcgcat cctgcgcgtt
     5101 actggcatac atcagcgcgc aactgtccca ttccagccat gcatttttgt cctcacctaa
     5161 atagctgcta aacgctttga ttccccacgg gacgctgcac ggattcacaa ttggcgcaaa
     5221 ggccgaaacg ctggtgtatt tacccggatt tttcagcgcc ataatcagcg caccgtgacc
     5281 acccattgaa tgaccgctaa tggcgcagcg gtcgctgaca ttaaattgcg actgaaccag
     5341 cgccggtaat tcatcgcgca gataatcata catccggtaa tgcgtcgccc acggcggttg
     5401 cgtggcgtta agataaaacc ccgcgccctg gcctaaatcg tagccatcat cgttggcaac
     5461 ctgttcgccg cgcgggctgg tgtctggcat caccagtaca atccccagtt ctgccgccac
     5521 gcgctgggca cccgccttga tggtgaagtt ctcgtcattg caggttaatc cggaaagcca
     5581 gtacagcact ggtggcgggg tgtgatcacg aggtggtggg agaaagatac tgaacgtcat
     5641 cggacagttt aaggtactgg agtcgtgtcg ccagcgttgc tgccagcctt caaaacagcg
     5701 gtgctcttcg agcatttcca tgcgtggctc cttgttgtgt tgtttgcaat ttcttcatca
     5761 taccgtttat tcaatgtgct gtgagtaact ttcacttccg tatttgcata acgatgtttt
     5821 aacatctgct gatgaaaggc agcggcaatt acaataatta tcgctgtgaa tactggatta
     5881 tgtgcgccgc ctcacgcaca ataatcaagc tgtaaatcag cttaataact ttgcccccac
     5941 gcagggcgga ggcgtcacac ctgcaggaga aatcataaat gccatcactc agtaaagaag
     6001 cggccctggt tcatgaagcg ttagttgcgc gaggactgga aacaccgctg cgcccgcccg
     6061 tgcatgaaat ggataacgaa acgcgcaaaa gccttattgc tggtcatatg accgaaatca
     6121 tgcagctgct gaatctcgac ctggctgatg acagtttgat ggaaacgccg catcgcatcg
     6181 ctaaaatgta tgtcgatgaa attttctccg gtctggatta cgccaacttc ccgaaaatca
     6241 ccctcattga aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat atcactctga
     6301 ccagtacctg tgaacaccat tttgttacca tcgatggcaa agcgacggtg gcttatatcc
     6361 cgaaagattc ggtgatcggt ctgtcaaaaa ttaaccgtat cgtgcagttc tttgcccagc
     6421 gtccgcaggt gcaggaacgt ctgacgcagc aaattcttat tgcgctacaa acgctgctgg
     6481 gcaccaataa cgtggctgtc tcgatcgacg cggtgcatta ctgcgtgaag gcgcgtggca
     6541 tccgcgatgc aaccagtgcc acaacaacga cctctcttgg tggattgttc aaatccagtc
     6601 agaatacgcg ccacgagttt ctgcgcgctg tgcgtcatca caactgatta aaaggcagga
     6661 accatggagc gcaacgtcac gctcgatttt gttcgcggcg tcgccattct ggggatcctg
     6721 ctattaaaca tcagcgcctt tgggctacca aaggcggctt atctcaatcc cgcctggtac
     6781 ggtgctatta cgccgcagga tgcatggacc tgggcatttc ttgatctcat cgcccaggtg
     6841 aaattcctca cgctttttgc gctgctgttt ggtgctggac tgcaaatgtt gctgccccgt
     6901 ggcagacgct ggatccagtc gcggttaacg ctgttagtct tattgggctt tattcacggt
     6961 ttattgttct gggacggcga tattctgctg gcttacgggc tggtgggctt aatctgctgg
     7021 cggctggtgc gcgatgcgcc atcggtaaaa agcctgttta atacaggcgt catgctttat
     7081 ctggtggggc ttggcgtttt gctgttattg gggctgattt ccgacagcca gaccagccgc
     7141 gcctggacgc cggatgcatc ggctattttg tatgaaaaat actggaagct tcacggcggc
     7201 gttgaagcga tcagtaatcg tgccgatggt gttggcaaca gtttactggc actgggcgca
     7261 cagtatggct ggcaactggc tgggatgatg ctcattggtg cagcgctgat gcgcagcggc
     7321 tggctgaaag ggcagttcag cttacgtcac tatcgtcgta ctggttttgt gctggtggcg
     7381 attggggtga ccattaacct tcctgccatc gccctgcaat ggcagctgga ctgggcgtat
     7441 cgctggtgtg ccttcttact tcaaatgccg cgggaactga gtgcgccgtt tcaggcgatt
     7501 ggctatgcgt cgctgtttta tggcttctgg ccgcaattga gccgctttaa gctggtgctt
     7561 gcgatcgcct gcgtcggacg gatggcgctg accaactatc tattgcaaac gctgatttgt
     7621 accatgcttt tttaccacct cggtttgttt atgcattttg accgcctgga gctgctggcg
     7681 tttgttattc cggtatggct ggcgaatatc ctcttctctg ttatctggct gtgttacttc
     7741 cgccaggggc cggtggaatg gctctggcgt cagttaactt tgcgtgctgc cggaacggca
     7801 atatctaaaa catcaagata acgatctgga tcacattcgt taacaaaacg gctgtaaccg
     7861 tttccattgc tgtgactcga ttcacgaagt cctgtattca gtgctgacaa aatagccgcc
     7921 agcaagcagt catttactgc aatctcataa caggtagtga atatgatcac cattcgtgat
     7981 gtagcgcgtc aggctggcgt ctctgtggca acggtttccc gggtgctcaa taacagcacg
     8041 ctggtcagtg ccgacacgcg tgaagcagta atgaaagccg tgagtgagct ggattatcgg
     8101 ccaaacgcca atgcccaggc gctggcaact caggttagcg acaccattgg cgtggtggtg
     8161 atggacgttt ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct ggtcgctcag
     8221 cagcatcaga aatacgtgct aatcggcaat agctatcatg aagcggaaaa agagcgtcac
     8281 gccattgagg tgttaattcg ccagcgttgt aatgcgttga ttgttcactc aaaagcattg
     8341 agtgacgatg aactggcgca atttatggat aacattcccg gtatggtgtt aatcaaccgc
     8401 gttgtgccgg ggtacgccca tcgttgcgtt tgcctggata atctcagcgg tgcccgaatg
     8461 gcgacgcgca tgttgctgaa taacggtcat caacgtattg gttatctttc ttccagtcac
     8521 ggcattgaag atgacgccat gcgtaaagca ggctggatga gtgcgttgaa agagcaggat
     8581 attattccgc cggaaagctg gattggcact ggtacgccgg acatgccggg cggtgaggcg
     8641 gcgatggttg aactgctggg gcgcaatcta caacttaccg ctgtatttgc ttataacgac
     8701 aatatggctg ctggcgcact gacagcatta aaagataatg gcattgcgat tccgttacat
     8761 ctctcaatca tcggtttcga tgatattccc atcgcccgtt acaccgaccc gcaattaacg
     8821 accgtgcgtt atcccattgc ttcaatggcg aaattagcca ccgaactggc cttgcagggg
     8881 gcagcaggca atattgatcc tcgtgctagc cactgtttta tgccgacgtt agtgcgtcgc
     8941 cattctgtcg caacacgcca gaatgcggcg acgatcacta actcaacaaa tcaggcgatg
     9001 taaccgcttt caatctgtga gtgatttcac agtatcttaa caatgtgata gctatgattg
     9061 caccgtttta acgttgtaac ccgtatgtaa cagtgaataa tcactttcac cgaggtaata
     9121 gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga agaacgaatt
     9181 ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgccc cgccgagcat
     9241 ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa gaaggtgtta
     9301 accctgtctg ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc tgctgatact
     9361 cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtagt gcgcaaggct
     9421 attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga ttctcagaat
     9481 gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt gaaggcactg
     9541 gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc gcgtgggcaa
     9601 aacgtgccgg tggttttctt caacaaagaa ccgtctcgta aggcgctgga tagctacgac
     9661 aaagcctact acgttggcac tgattccaaa gagtccggca ttattcaggg cgatttgatt
     9721 gctaaacact gggcggcgaa tcagggttgg gatctgaaca aagacggtca gattcaattc
     9781 gtattgctga aaggtgaacc gggccatccg gatgcagaag cacgtaccac ttacgtgatt
     9841 aaagagctga acgacaaagg cattaaaact gaacagttac agttagatac cgctatgtgg
     9901 gataccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa cgccaacaaa
     9961 atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga agcactgaaa
    10021 gcacacaaca agtccagcat tccggtgttt ggcgtcgatg cgctgccaga agcgctggcg
    10081 ctggtgaaat ccggtgcact ggcgggcaca gtactgaacg atgctaacaa ccaggcgaaa
    10141 gcgacctttg atctggcgaa aaacctggcc gatggcaaag gtgcggctga tggcaccaac
    10201 tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa agacaacctg
    10261 gctgaattca gcaagaaata agactgattg ggcgcagtcg tttactgcgc ccttattgta
    10321 tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg ggaatacttg
    10381 ttggaaatga gcggtatcaa caagtccttt cctggtgtta aggcacttga taacgttaat
    10441 ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggtgc aggaaaatcg
    10501 acattattaa aatgcctgtt tggtatttat caaaaagact ccggcaccat tttattccag
    10561 ggtaaagaga tcgatttcca ttccgccaaa gaggcactgg aaaatggtat ttcgatggta
    10621 caccaggagt taaacctggt attacaacgt tcggtgatgg ataacatgtg gctggggcga
    10681 tatcccacca aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac caaagcgatt
    10741 tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt atccgtttcg
    10801 caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat tgtgattatg
    10861 gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac tattattcgt
    10921 aaattaaaag agcgcggctg cggtattgtt tatatctcgc ataaaatgga agaaatcttc
    10981 cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac cgaaccgctg
    11041 gcaggactga cgatggacaa gatcatcgcc atgatggttg ggcgttctct taatcagcgt
    11101 tttcctgaca aagaaaacaa gccgggcgaa gtcatcctcg aggtgcgtaa cctaacgtca
    11161 ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga gatcctcggt
    11221 attgccgggc tggtgggggc gaaacgtacc gatattgttg agacgttatt tggtattcgc
    11281 gagaaatcgg ctggcaccat tacgttgcac ggcaaacaga tcaataacca taatgccaac
    11341 gaagccataa accacggatt tgcactggta actgaggagc gccgctcaac gggaatttat
    11401 gcctatctgg atattagttt taactcgtta atttccaata ttcgcaacta caaaaataaa
    11461 gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat tgattcgatg
    11521 cgggtaaaaa cgccgggtca tcggacgcaa attggttcgc tctccggtgg taatcagcaa
    11581 aaggtgatta ttggtcgctg gctactaacg caaccagaaa tattaatgct cgatgaaccg
    11641 acgcgcggta ttgatgtcgg ggcgaagttt gaaatttatc agttaattgc cgaactggcg
    11701 aagaaaggca aggggattat tattatctcc tctgaaatgc ctgagttgtt agggataaca
    11761 gaccgtattc tggtcatgag caatggtctc gtttccggaa ttgtcgatac aaaaacaaca
    11821 acgcaaaacg aaattctgcg tcttgcgtct ttgcaccttt aagattaggg gcttcccatg
    11881 agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat ttacgtcgtt
    11941 cttttagttt tgctggcgat tattattttc caggacccaa catttttaag tctgttgaac
    12001 ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg tgtggcaggg
    12061 ttaattgtca cccaggggac cgatctttct gctggtcgtc aggtagggct ggcggcagtg
    12121 gtggctgcga cattattgca gtccatggat aacgccaaca aagtgttccc ggaaatggcg
    12181 acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt gatcggtttg
    12241 atcaacggtc tgattatcgc ttatctcaac gtgacgccgt tcattaccac gctcggcacg
    12301 atgatcatcg tctatggaat caactcgctc tattacgact ttgtcggggc gtcgccaatt
    12361 tctggttttg acagtggctt ctctaccttt gctcagggct ttgtcgcgct ggggagtttc
    12421 cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg ggtgttgtgg
    12481 aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga agcggcaaaa
    12541 gtatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc tggcgtgttc
    12601 tatgcctttg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa caacctcggc
    12661 tttatgtatg agctggatgc tatcgcggcg tgcgtggtag gcggtgtatc gttcagcggc
    12721 ggtgtgggga cggtgattgg cgtggtgacc ggggtaatta tttttaccgt catcaactat
    12781 ggcctgacgt atatcggcgt aaacccatac tggcagtaca tcatcaaagg ggcgattatt
    12841 atcttcgccg tagcgctgga ttcactgaaa tacgcgcgta agaagtgatg tgattgtgga
    12901 tattgccgga tgcgctgcat ccggcaatcg gtgccaaatg ccggatgcgt ctctggtttt
    12961 acaacgttac cgggtgttct ttctcgcctt tcttaaactt cacttccccg agctcaatac
    13021 aacccaccgg gcagacgtga ccacaaagca gacaacccac acatttctcg gtattacaat
    13081 gcggtgtgcg ggttttctcg ctccattcca tcgcctggtg accgccgtcg taacaggaaa
    13141 tataacagcg tccacagcca acacatttat caagattgat acggggatag acaatataac
    13201 tgcggtctaa atcttccgcc gggacaatat tgttattcgc cagacctacc atctcctgca
    13261 gcgaatcaaa tccctgatcg gcgagataat gcgacaaccc gcttgccata tcttccacta
    13321 tccgataccc gtactgcatg atgccggtgg tcacctgtaa cgttgctgcg ccgagcaata
    13381 aaaactcagc cgcatcctcc caggtttcaa tgccgccgat accgctgatt gggaaatcgc
    13441 gcagttctgg atgggtgcgc atctgctgaa tgaagcgcag ggcgatcggt tttaccgctt
    13501 taccggaata tccggaaata ctcgattttc cgttaacgat cggcataccg attttctgat
    13561 taagatcgat attggtgatg gatttaacgg tgttaatcgc cgcaatgcca tctgcgccgc
    13621 cgcgcttcgc cgccagcgcc acttcgcaca tatcaccgat attcggcgtc atcttcgcca
    13681 gcattggcag cgtggaaccc cgtttcactg cccgacaata tttttctacc agctccgggc
    13741 tttgcccgac atcgctaccc atcgcatgag aagtcatttg cggacaggag aagttacact
    13801 cgatcatatc cgcgccagct tcttgcacca ggcgcgccag ctcctcccat tgctgctcat
    13861 tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc agctgacgca
    13921 gggcggccag attctcttcc aacggatgtt cagcaatctg ctccatattt ttgaagccga
    13981 taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg ttggcgataa
    14041 aaaagccgat cgttttaaac accacgccgc cccaacctgt gtcgtaggct ttggcacaca
    14101 tctcatagca gttgcctaca ggcgaagaag agaggcagaa cgggttggga aacttcacgc
    14161 cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta aatagtgatg
    14221 aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt tgtcaccctc
    14281 aacaatatcg ccagcagcaa agacttgcgg atcgcgggtc tgataatttt gtgttttgat
    14341 ggtgttacgc tgcggctcta actccgcaaa ggcatccagt ctggcatgct gaccgacggc
    14401 gagaataatt ttatctgccg ccatcgtcag ttcgcccggt aaccgtacat gcttaaacgt
    14461 gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa cgcccagctc
    14521 ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt cacgcgctac
    14581 gcaggttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca tcgcaacatc
    14641 accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc
    14701 acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg ataacccgga
    14761 accgctcgat aaccccacgg tgaccagtac cgcgcggttt tctgctttca gctgctcaag
    14821 ggtgagtgtg ttaccgactt cgttgttgca cttaatggtc acgcccattt tttcaatacg
    14881 ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac cgttacgcaa
    14941 ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct ggtttgtcag
    15001 tgttacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt tgccgagcgt
    15061 tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag taacaaaacg
    15121 ctgtaagcga ccgatatcaa tgggagtatc aacaccggca cgggtacaac cgctttgaca
    15181 taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat tttcgcgaat
    15241 tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt tccccggatc
    15301 ggtctgtgcc gggcaagcct gactacaggg agcgtcatga cataataaac agcgagaggc
    15361 ttctttaatc gccagtaaag gcgtaaaagc cggagtgagt tcatccagat aattttgttg
    15421 cggcatgtga tcttccttaa tgctatgagt aattaaggcg aacgctaaat atactcgtca
    15481 tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag
    15541 ctcctgggga ctcactcact tgtcgccttc ctgcaactcg aattatttag agtataaata
    15601 ttgcgtgctg atattaaggc tatgcgcgtg gggaaattta tactgtgaaa ggattcacaa
    15661 atagctgcgg ttcagaatgt tatatccgca gcttattgag tgaagtaa
//
LOCUS       DAZFKM010000114        15677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFKM010000114 DAZFKM010000000
VERSION     DAZFKM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..409
                     /gene="yiaB"
                     /locus_tag="ACHKAY_003747"
     CDS             68..409
                     /gene="yiaB"
                     /locus_tag="ACHKAY_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO5702345.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            455..892
                     /gene="yiaA"
                     /locus_tag="ACHKAY_003748"
     CDS             455..892
                     /gene="yiaA"
                     /locus_tag="ACHKAY_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO5702346.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(934..1929)
                     /gene="wecH"
                     /locus_tag="ACHKAY_003749"
     CDS             complement(934..1929)
                     /gene="wecH"
                     /locus_tag="ACHKAY_003749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO5702347.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLFLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            2104..2403
                     /gene="ysaB"
                     /locus_tag="ACHKAY_003750"
     CDS             2104..2403
                     /gene="ysaB"
                     /locus_tag="ACHKAY_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO5702348.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2498..3409
                     /gene="glyQ"
                     /locus_tag="ACHKAY_003751"
     CDS             2498..3409
                     /gene="glyQ"
                     /locus_tag="ACHKAY_003751"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO5702349.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3419..5488
                     /gene="glyS"
                     /locus_tag="ACHKAY_003752"
     CDS             3419..5488
                     /gene="glyS"
                     /locus_tag="ACHKAY_003752"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO5702350.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            5813..5965
                     /gene="hokA"
                     /locus_tag="ACHKAY_003753"
     CDS             5813..5965
                     /gene="hokA"
                     /locus_tag="ACHKAY_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO5702351.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(5954..6013)
                     /locus_tag="ACHKAY_003754"
     CDS             complement(5954..6013)
                     /locus_tag="ACHKAY_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702352.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(6153..6365)
                     /gene="cspA"
                     /locus_tag="ACHKAY_003755"
     CDS             complement(6153..6365)
                     /gene="cspA"
                     /locus_tag="ACHKAY_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO5702353.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(6646..6936)
                     /gene="yiaG"
                     /locus_tag="ACHKAY_003756"
     CDS             complement(6646..6936)
                     /gene="yiaG"
                     /locus_tag="ACHKAY_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO5702354.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            7370..8080
                     /gene="yiaF"
                     /locus_tag="ACHKAY_003757"
     CDS             7370..8080
                     /gene="yiaF"
                     /locus_tag="ACHKAY_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO5702355.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(8130..9104)
                     /gene="ghrB"
                     /locus_tag="ACHKAY_003758"
     CDS             complement(8130..9104)
                     /gene="ghrB"
                     /locus_tag="ACHKAY_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO5702356.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(9208..9867)
                     /gene="yiaD"
                     /locus_tag="ACHKAY_003759"
     CDS             complement(9208..9867)
                     /gene="yiaD"
                     /locus_tag="ACHKAY_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO5702357.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            10020..12353
                     /gene="bisC"
                     /locus_tag="ACHKAY_003760"
     CDS             10020..12353
                     /gene="bisC"
                     /locus_tag="ACHKAY_003760"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO5702358.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASLQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(12322..12762)
                     /gene="yiaC"
                     /locus_tag="ACHKAY_003761"
     CDS             complement(12322..12762)
                     /gene="yiaC"
                     /locus_tag="ACHKAY_003761"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO5702359.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(12759..13322)
                     /gene="tag"
                     /locus_tag="ACHKAY_003762"
     CDS             complement(12759..13322)
                     /gene="tag"
                     /locus_tag="ACHKAY_003762"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO5702360.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            13480..14178
                     /gene="yhjY"
                     /locus_tag="ACHKAY_003763"
     CDS             13480..14178
                     /gene="yhjY"
                     /locus_tag="ACHKAY_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO5702361.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            14407..15609
                     /gene="yhjX"
                     /locus_tag="ACHKAY_003764"
     CDS             14407..15609
                     /gene="yhjX"
                     /locus_tag="ACHKAY_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5702362.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         3827 a         3814 c         4034 g         4002 t
ORIGIN      
        1 tggcattttt cagttttgtc tttcgttggt tactcgtaat gtatcgctgg tagatatgga
       61 gatcgttatg aaaacctcaa aaactgtggc aaaactatta tttgttgtcg gggcgctggt
      121 ttatctggtt gggctatgga tctcatgccc attgctaagt ggaaaaggct attttcttgg
      181 cgtgttaatg acagcaactt ttggtaacta tgcatatctt cgcgcagaaa aactcgggca
      241 actggataat ttttttaccc atatctgcca gttagttgcg ttaatcacta tcggtctctt
      301 gtttatcggt gttttaaatg cacctatcaa tgcttatgaa atggtgatct accccatcgc
      361 cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc tcggtatgag caacataaag
      421 ctcttacaca ttcaggaatg aaaggaatac tgtgatggac aacaaaatat caacctattc
      481 accggccttt agtattgtgt catggatagc tctcgttggt ggtatcgtta cctatctgtt
      541 agggctatgg aatgcagaga tgcagttaaa tgaaaaaggg tattattttg ccgtactggt
      601 gttaggactg ttttctgctg cgtcttatca aaagaccgtt cgggacaagt atgaaggcat
      661 accgaccact tccatttatt atatgacctg cctgactgtc tttattatct ctgttgcatt
      721 actgatggta ggtctgtgga atgcgacatt attactcagt gaaaaaggtt tttatggact
      781 ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag aagaatattc gtgataccgg
      841 aataaacccg ccaaaagaaa cacaggttac ccaggaagaa tacagcgaat aattcacgta
      901 agcccggtca gtccaatgtg aacgggcttt tacttaactc actaatctgt ttctgtcgat
      961 tcgttgtacc agcatagaaa gtaacaaact cgctgccaac gtcgcgcaaa agatccaaat
     1021 aatatccagt attggccaat ttttaagctc aattccccgg gtgcgcagcg catggataat
     1081 caaggcgtgg aatccgtata tacccaatga atggcgggag attaagccaa gtccgcgaat
     1141 ggtacgcgta tccagcgtgt ttttaaccag agtcaatagc gcgattgcgc agataaaaac
     1201 catcggccca cagtaaagat accaggtatc ggcaaaattt ccgcgccact gcaattcata
     1261 taatgtcccg cgagagataa taaaaacccc cgtcgcaaac agcgcggcgc tcacccacga
     1321 cagtgcttta tgctgtgtgt ccatcatccc tatagcgcgg cccaacatgc catacagaat
     1381 gtagtaaaaa gtatcgccat tgatatataa gttaattggc agccattcaa aaccgtcaat
     1441 tttctgcggc actgtgtttg ggttagcgat aatgccaatc accaccatta gcaccagcaa
     1501 catttttccg ccgacgttct tcacctgaat cagcggtgaa accagataaa tcaccgcaat
     1561 cgcgaagaaa aaccacaagt ggtaaaacac tggcttttgc agcaggtttt tcagcgctaa
     1621 ctccatattg atggaggtaa acagcgcaat gtagagcagt gcgattgcgc tataaaaaat
     1681 cagaaataag ccgatacgca agaaatggcg cggctgggcg ctgcgttcgc caaaaaagag
     1741 atagccggaa atcatgaaaa atagcggcac gctgacacga gaggcagaat tcagaacatt
     1801 ggcgatatcc catgtgacgg ggctaacact atgagcattg gtcacatacc aggtagtggt
     1861 gtgaatcatc accaccatta aacacgctat ccctcgcagg ttatcaatcc agtaaatttt
     1921 gggctgcatc tgtgtctctg tatctggtta aaaaaagtct gaccgataaa tcatttggtt
     1981 ggcgcactgg aataatctga gttttatcac tacagcttat agaggcttaa ggaaattcgt
     2041 aagatatcag ccactatacc gatataaata ataagactca cctgcaaacc agacggtaat
     2101 ttaatgatga tgaacgcttt ctttccggca atggcgctta tggtgctagt gggttgttct
     2161 acaccgtcac ccgtgcagaa agcacaacgg gtaaaggttg atcctctgcg ttcgttgagt
     2221 atggaagcgt tatgcaagga tcaggcggca aaacgttata acaccggcga gcaaaaaatc
     2281 gacgtcaccg ccttcgaaca gttccaggga agctatgaaa tgcgcggtta taccttccgt
     2341 aaagagcagt ttgtctgttc ttttgacgca gatggccatt ttttgcatct ttccatgcgt
     2401 taagccctgc tttttcccgt ttcgtactgt atatcttcca tccagcgggt atactgatcc
     2461 cttcctttaa atccacacgt atccagcacg aaataatatg caaaagtttg ataccaggac
     2521 cttccagggc ttgatcctga ccttacagga ttactgggct cgccagggct gcaccattgt
     2581 tcaaccattg gacatggaag tcggcgcggg aacctctcac ccaatgacct gtctgcgcgc
     2641 gctggggcca gaaccgatgg cggctgctta tgttcagcct tctcgtcgcc cgaccgatgg
     2701 tcgctacggc gaaaacccca accgtttaca gcactactat cagttccagg tggtcattaa
     2761 gccatcgccg gacaatattc aggagctgta cctcggttct ctgaaagagc tgggcatgga
     2821 cccgactatt cacgacatcc gtttcgtgga agataactgg gaaaacccga ctctgggtgc
     2881 ctggggactg ggctgggaag tgtggctaaa cggcatggaa gtgacgcagt tcacttactt
     2941 ccagcaggtt ggtggtctgg agtgtaaacc ggttaccggc gagatcacct acggtctgga
     3001 acgtctggcc atgtacattc agggcgtaga cagcgtttac gacctggtct ggagcgacgg
     3061 cccgctgggt aaaaccacct acggcgacgt gttccatcag aacgaagtgg agcagtccac
     3121 ttacaacttc gaatacgcgg atgtggactt cctgttcacc tgctttgagc agtacgagaa
     3181 agaagcgcag cagctgctgg cgctggaaaa tccgctgccg ctgccagcct acgagcgtat
     3241 tctgaaagcc gcccacagct tcaacctgct ggatgcgcgt aaagccatct ccgtcaccga
     3301 gcgtcagcgc tacattctgc gcattcgcac cctgaccaaa gcagtggcag aagcatacta
     3361 cgcttcccgt gaagccctcg gcttcccgat gtgcaacaaa gataagtaag aggcggctat
     3421 gtctgagaaa acttttctgg tggaaatcgg cactgaagag ctgccaccaa aagcactgcg
     3481 cagcctggct gagtcctttg ctgcgaattt tactgcggag ctggataacg ctggcctcgc
     3541 acacggcacc gttcaatggt ttgctgctcc gcgtcgtctg gcgctgaaag tagctaacct
     3601 ggcggaagcg caaccggatc gtgaaatcga aaaacgcggc ccggcgattg cccaggcgtt
     3661 tgacgctgaa ggcaaaccga gcaaagcggc agaaggttgg gcgcgtggtt gcggtattac
     3721 cgttgaccag gctgagcgtc tgactaccga taaaggcgaa tggctgctgt atcgcgccca
     3781 tgtgaagggc gaaagcaccg aagcactgct gccgaatatg gttgcgactt ctctggcgaa
     3841 actgccgatc ccgaaactga tgcgttgggg cgcaagcgac gtgcacttcg tgcgtccggt
     3901 gcacaccgtg accctgctgc tgggcgacaa agtcattccg gcaaccattc tgggcattca
     3961 gtccgatcgc gtgattcgcg gccaccgctt tatgggcgaa ccggaattca ccatcgacaa
     4021 cgccgatcag tatccggaaa ttctgcgtga gcgcgggaaa gtcatcgccg attacgaaga
     4081 acgtaaagcg aagattaaag ccgatgccga agaagcagcg cgtaagattg gcggtaacgc
     4141 tgacttaagc gaaagcctgc tggaagaagt ggcttcgctg gtggagtggc cggtcgttct
     4201 gaccgcaaaa ttcgaagaga aattcctcgc ggtgccggct gaagccctgg tttacaccat
     4261 gaaaggtgac cagaaatact tcccggtgta tgcgaacgat ggcaaactgc tgccgaactt
     4321 catcttcgtt gccaacatcg aatcgaaaga tccgcagcaa attatctctg gtaacgagaa
     4381 agtcgttcgt ccacgtctgg cggatgccga gttcttcttc aataccgacc gtaaaaaacg
     4441 tctggaagat aacctgccgc gcctgcaaac cgtgctgttc cagcaacagc tgggtacgct
     4501 gcgcgacaaa actgaccgca tccaggcgct ggctggctgg attgctgaac agattggcgc
     4561 tgacgttaac cacgcaaccc gtgcgggcct gctgtctaag tgtgacctga tgaccaacat
     4621 ggtcttcgag ttcaccgaca cccagggcgt tatggggatg cactatgcgc gtcacgatgg
     4681 cgaagcggaa gatgtcgcgg tggcgctgaa tgagcagtat cagccgcgct ttgccggtga
     4741 tgacctgccg tctaacccgg tagcctgtgc gctggcgatt gctgacaaga tggatactct
     4801 ggcgggtatc ttcggtatcg gccagcatcc gaaaggcgac aaagacccgt ttgcgctgcg
     4861 tcgtgccgca cttggcgtgc tgcgaattat cgttgagaag aacctcaacc ttgatctgca
     4921 aacgctgacc gaagaagcag tgcgtctgta tggcgataag ctgactaacg ccaacgtggt
     4981 tgatgatgtt atcgacttta tgctcggtcg cttccgcgcc tggtatcagg acgaaggtta
     5041 caccgttgac accatccagg cggtactggc gcgtcgtccg actcgtccgg ctgatttcga
     5101 tgcccgcatg aaagcggtat cgcacttccg tacccttgaa gcagctgctg cactggcggc
     5161 ggcgaacaag cgtgtctcca acattttggc gaaatctgac gaagtgctga gcgaccgcgt
     5221 gaatgcctct accctgaaag agccggaaga aattaaactg gcgatgcagg ttgtggtgct
     5281 acgtgacaag ctggagccgt actttgcgga aggtcgttac caggatgcgc tggtcgaact
     5341 ggcagagctg cgtgagccgg ttgatgcttt cttcgataaa gtgatggtca tggttgatga
     5401 caaagaattg cgtctcaacc gtctgaccat gctggagaaa ctgcgcgaac tgttcctgcg
     5461 cgttgcggat atttcgctgt tgcagtaata acgccgttat taaaaagcct gccatctggc
     5521 aggctttttt tattatcgct aaataataca gcaaccttta ataatcttct gctgaataaa
     5581 gattatctca tatattaatt ttatgaggtt ttttaggatt atatcaagga gaagaaacaa
     5641 acttattaag ctagaatagc cacgggtgct tgagactgtt tgtctcaggt attcaccgaa
     5701 aggcagacag agaaaagccc cacctgacta taaatcaaag tgaggctacc ctatgcctga
     5761 acaccataag gttagcctct tactcgttgg aaatcaacac agggggctgg gaatgccgca
     5821 gaaatataga ttactttctt taatagtgat ttgtttcacg cttttatttt tcacctggat
     5881 gataagagat tcactgtgtg aattgcatat taaacagggg agttatgagc tggcggcatt
     5941 tttagcctgc aatttaaaag agtaagagtc ttcggcggga agttattccc gcctttctta
     6001 cggcgttgcg cattctcatt gcacccaaat ttatttttca caaaaataat actagatttt
     6061 attacgcgat cgattattta tttcctgaaa aacaaataaa aaaatccccg ccaaatggca
     6121 gggatcttag attctgtgct tttaagcaga gattacaggc tggttacgtt accagctgcc
     6181 gggcctttag cgccgctttc gatggtgaag gacactttct gaccttcgtc cagagatttg
     6241 taaccatcgt tctggatagc agagaagtgt acgaacacgt ctttagagcc atcgtcagga
     6301 gtgatgaagc cgaagccttt gtcagcgttg aaccatttta cgataccagt cattttaccg
     6361 gacatagtgt attaccttta ataattaagt gtgcctttcg gcgatatggc gtgctttaca
     6421 gattttgaag cgttaaagga atgtgcacta cgaggggtat caacgataac tcttgaaggg
     6481 acttgcctta ctacactgct ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc
     6541 acgcattggc gggtgatgca acaattattt ttcatattta tgattaatcg gttggaaaac
     6601 ggtcctgtca tcaggaccgt aaacagcaat aaagtggata aaagtctatt ccatcaactg
     6661 cttacttaat gccgggttgg cttgaatcaa acgcatcaat tttagttcgg cacttgaagg
     6721 cttcacgcgt ctggattccc attcctttac catggcgact gatacgccca aaacccgggc
     6781 gaaatcatcg atttttaacc ctgtcccttt tcgtaactgc tcaatttcgg tacaggacgt
     6841 tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta aaaacaatct gttccaggct
     6901 gctcaacagc tcatgcattg gatctttata ttccattgaa aactcctcaa atcacactgc
     6961 gggatcgtga attgcatcga agctcattaa gaatagtcgg gaaaagcaga cacagcgggg
     7021 tgaagtcagg gcatgctctg gctatgattc gattcagcca gtcatttagc gtgacttata
     7081 gccacctaac gatctgataa atgccgatat atctcttacc actcctggtt tgtaaagatt
     7141 tcgaaaatct aaccttctgc gaaggcgtaa atctctggct acaaaaaaag caacggcact
     7201 atcatttttt aacaatgaaa caacaaatta accttgtttc attttctcca tgattttacc
     7261 aggaagatta cgtagaaagg gtatcctgca agcctgtcct ggactatcct tgtcacgtca
     7321 gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg
     7381 aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag
     7441 tgggtgtttt gataaagaag gcgatcagcg taaagcgttt atcgacttcc tgcagaatac
     7501 agtgatgcgt agcggtgaac gtctaccaac cctgactgcc gatcagaaaa aacagtttgg
     7561 tccttttgtc tctgattacg cgattctgta tggttattct cagcaggtga atcaggcgat
     7621 ggattccggt ctgcgcccgg tcgtagacag cgttaacgca attcgcgtac cgcaggatta
     7681 cgttacgcaa agcggtccgc tgcgtgaaat gaacggttcg ctgggtgtgc tggcgcaaca
     7741 actgcaaaat gcgaagttgc aggccgatgc tgcgcactct gcgctaaaac agaccgatga
     7801 tttgaaaccg gtctttgatc aggcattcac caaagtggta acgacgccag ctgatgcatt
     7861 gcaaccgttg attccggctg cacaaacttt cacgcaacaa ctggtgatgg ttggagacta
     7921 cattgctcag caaggtactc aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc
     7981 acagcaggca agtgaatata acaaactgat tgcgccatta ccggcacaac atcaggcgtt
     8041 taatcaggcc tggactacag cagttactgc aacccaataa agagtaaaag cccgagcgat
     8101 tatcgggctt tacttcacgc agtcgcggct tagtccgcga cgtgcggatt cacacagttc
     8161 ttctcaacct ttccttgtaa cgcatcaatc aaattatcca cggcacaggc ggccatgcca
     8221 taacgcgtct catgggtggc agatccaata tgcggtactg cgacgacgtt ggccattgag
     8281 agcaacggcg aatctacgga cagtggctct tgttcgaaga catccagccc ggcggcgtga
     8341 atttcgcctt tctgcaatgc agcaatcagt gcattttcat caaccaccgg gccacgtccg
     8401 gcattaatga aaatggcgga ggatttcatt ttggcgaatt gttctgcgcc aaacagatga
     8461 tgcgtctcat cagttaacgg caggatcagg caaacgaaat ctgactcttg taacagagta
     8521 tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg
     8581 ttatagagga tgggcatgtt gaagccaaag tgcgcacgtt gtgccagcgc cataccgatc
     8641 cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta ccagtccggg
     8701 cctatgctcg cggtccattc gcctgctttt acccgttctg ctacctccac aacccgacga
     8761 gcggtagaca acaccagcgc catcagcgta tcggcgacgg tttctgttaa tacggttggc
     8821 gtgtgcatca gcagaatttt tcgggcggta agcgcatcga catcaaaatt gtcatagccg
     8881 acggagatcg ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc ggcatcaaca
     8941 ttctcgtttg aacccagtaa accttcagct tcggcaaaaa ttgctgcatt ttgttcgacg
     9001 gtttgtgggc tgaggtttgc cacctggtga acggtgaaat gctcttgcag gcgttgcagt
     9061 aaatcatcag gtaaggcttt gtagaggata acggacggct tcatgcttct ctccattcac
     9121 tgataaggcg atcaccttag cctgaaatat gatgaaggaa aactgaagaa tgccaggtga
     9181 tttctgcatc acctggcatg aaagggatta cagcgggctt aaggtaattt ctacacggcg
     9241 gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt gggttagccg ggccaaggcc
     9301 ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc
     9361 cgcacgttgc tgggagagac gcatgttcag gtcgtgacca cccgtgctgt cggtataacc
     9421 aatcacgtta accgccgttt tcggatactc tttcagcacc attgccacgc cggtcagggt
     9481 gttagcgccc gcaggtttca gggtcgcgct gctgctgtcg aaggtcacat tgttcggcat
     9541 attgaggata atgttatccc cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc
     9601 acgcagcttc gcttcctgca catccatgta ataacccacg ccgccgccca gagctgcgcc
     9661 tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc ttcgaagaag agagcgcacc
     9721 aatacccgcg cccacgagag agcccagacc tgcgccgata gcagatttac ctgcttcgcg
     9781 ttcgccggtg taagggttag ttgtgcagcc agataccgcc agagcgccac tcactacggc
     9841 ggcaataaga taaacacgtt tcttcattgt taatccttaa taaccttttt attctttgcc
     9901 acgggttccg tggcgggaga ttatgccgcg tgaacatgaa gattattcct gggaatactc
     9961 ggaaatttgt aagtaatatt taactgctca atacatctaa cctttcagga gttttcggtt
    10021 tggccaactc atcctcacga tattccgttc tgactgccgc ccattggggg cccatgctgg
    10081 ttgaaaccga cggcgaaacc gtgtttagct cgcatggcgc gttagccaca ggaatggaaa
    10141 actccttgca gagcgcggtt cgcgaccagg ttcacagcaa tacacgggta cgatttccaa
    10201 tggtgcgcaa aggctttctt gcgtcaccgg aaaatccgca aggcattcgt gggcaggatg
    10261 aatttgttcg cgtgagttgg gatgaggcgc tggatcttat tcaccaacaa cataaacgca
    10321 ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc ctacggctgg cgttcaaacg
    10381 gcgtgctgca taaggcctcg acattattac aacgctatat ggcgctggca ggcggttata
    10441 ccgggcatct gggggattat tcgaccggcg cggcacaggc gatcatgccg tatgtcgtgg
    10501 gtggtagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa catagcgatg
    10561 tcgtggtgtt gtggagtgct aacccactca atacgctgaa aattgcgtgg aatgcatctg
    10621 atgagcaggg gctttcttac ttttccgcac tgcgtgacag cgggaaaaag ctgatctgta
    10681 ttgatccaat gcgatcggaa accgtcgatt tctttggcga taaaatggag tgggtggcac
    10741 cgcacatggg caccgatgtt gcgctgatgc tggggatcgc ccatacgctg gtggaaaatg
    10801 gttggcacga cgaagcgttt ctgacgcgtt gcaccacagg ttatgccgtc ttcgcctctt
    10861 atttgctggg cgagagtgac ggaatagcga aaaacgccga atgggcggca gagatttgtg
    10921 gggttaacgc agcgaaaatc cgcgagctgg cggctctttt ccaccaaaac acgaccatgc
    10981 tgatggctgg ctggggaatg cagcgccaac agtttggtga gcaaaaacac tggatgatcg
    11041 tcacgctggc ggcaatgttg gggcaaatcg gcacacccgg cggcggtttt ggtctttctt
    11101 atcattttgc caatggtggt aaccccacgc ggcgttctgc ggtgctctct tccatgcagg
    11161 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
    11221 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
    11281 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
    11341 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
    11401 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
    11461 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
    11521 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
    11581 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
    11641 acgttgcccg gcagcgcggg gcaagcctgc aggttgaatt gccgccattt gctgagttct
    11701 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
    11761 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
    11821 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
    11881 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
    11941 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
    12001 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
    12061 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
    12121 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
    12181 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
    12241 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
    12301 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
    12361 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
    12421 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
    12481 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
    12541 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
    12601 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
    12661 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
    12721 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
    12781 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
    12841 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ccttagatag ggcgtcggag
    12901 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
    12961 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
    13021 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
    13081 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
    13141 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
    13201 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
    13261 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
    13321 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
    13381 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat gcaatgacaa
    13441 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
    13501 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
    13561 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
    13621 tacacctggg atagtgatca tcaacctgat tacaacgata ttttgtcgca acgtattcaa
    13681 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
    13741 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
    13801 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
    13861 actaccacgc tgaccgatcc gttatggcat gccagcgtga gtagcttagg ctggcgtgtt
    13921 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
    13981 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
    14041 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
    14101 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
    14161 gttagcgcca gattttaacg taacagtcac aattgaaacc attgaataac aacagttgtg
    14221 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
    14281 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
    14341 ataaaatgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
    14401 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
    14461 attacccagt ttgcactggg gtcggtttat acctggagcc tgtttaatgg cgcgctttct
    14521 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
    14581 ctggcaattt cgtcttctgt tgcgggcaaa ttgcaggaac gttttggcgt taaacgcgtc
    14641 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
    14701 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
    14761 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatatccgcg
    14821 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
    14881 ctggaaacgg tgggtctgga aaaaaccttt gtgatttggg gagcgattgc gctggtgatg
    14941 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
    15001 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
    15061 ttagcggtaa tgttcctgac cgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
    15121 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtcactgtt
    15181 atttccatcg ccaacctttc aggtcgtctg gtgctgggta ttctgtctga caaaatcgcc
    15241 cgtatccgtg ttatcaccat tggtcaggtg atatcgctgg tgggtatggc ggccctgctg
    15301 tttgcaccat tgaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
    15361 ggcggcacta ttaccgtctt tccgtcactg gtcagtgagt tctttggcct caataacctg
    15421 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttg tggttcgatt
    15481 atcgcctcac tgtttggcgg cttctatgtg accttctacg tgattttcgc cctgctgatt
    15541 ctgtcattgg cgctttctac gacaattcgt cagccagagc agaaagtatt gcgtgaggcg
    15601 catatttaga aatatgttgc tactgaaata aatgcgagga gggagacttc aaagtctccc
    15661 tttttttatg gacgcat
//
LOCUS       DAZFKM010000115        15507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFKM010000115 DAZFKM010000000
VERSION     DAZFKM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..692)
                     /locus_tag="ACHKAY_003765"
                     /pseudo
     CDS             complement(1..692)
                     /locus_tag="ACHKAY_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            693..1430
                     /gene="tagH"
                     /locus_tag="ACHKAY_003766"
                     /pseudo
     CDS             693..1430
                     /gene="tagH"
                     /locus_tag="ACHKAY_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
     gene            1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHKAY_003767"
     CDS             1427..1951
                     /gene="tssJ"
                     /locus_tag="ACHKAY_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO5702363.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            1954..3284
                     /gene="tssK"
                     /locus_tag="ACHKAY_003768"
                     /pseudo
     CDS             1954..3284
                     /gene="tssK"
                     /locus_tag="ACHKAY_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3289..4050
                     /gene="icmH"
                     /locus_tag="ACHKAY_003769"
     CDS             3289..4050
                     /gene="icmH"
                     /locus_tag="ACHKAY_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO5702364.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4059..6866
                     /gene="tssH"
                     /locus_tag="ACHKAY_003770"
                     /gene_synonym="clpV"
     CDS             4059..6866
                     /gene="tssH"
                     /locus_tag="ACHKAY_003770"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO5702365.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESEQDNTGAEPADEAGSEQPEETAETV
                     SPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDL
                     TKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAE
                     LLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDE
                     VEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQ
                     EVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIE
                     PEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGA
                     FTADVEDAQNDESVTKDETVL"
     gene            6863..7606
                     /gene="vasI"
                     /locus_tag="ACHKAY_003771"
     CDS             6863..7606
                     /gene="vasI"
                     /locus_tag="ACHKAY_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO5702366.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            7611..9023
                     /gene="tssA"
                     /locus_tag="ACHKAY_003772"
                     /pseudo
     CDS             7611..9023
                     /gene="tssA"
                     /locus_tag="ACHKAY_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            9132..12566
                     /gene="tssM"
                     /locus_tag="ACHKAY_003773"
     CDS             9132..12566
                     /gene="tssM"
                     /locus_tag="ACHKAY_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO5702367.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            12577..14026
                     /locus_tag="ACHKAY_003774"
                     /pseudo
     CDS             12577..14026
                     /locus_tag="ACHKAY_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            13953..14435
                     /locus_tag="ACHKAY_003775"
     CDS             13953..14435
                     /locus_tag="ACHKAY_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO5702368.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            14479..15393
                     /locus_tag="ACHKAY_003776"
     CDS             14479..15393
                     /locus_tag="ACHKAY_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702369.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            15403..>15507
                     /locus_tag="ACHKAY_003777"
     CDS             15403..>15507
                     /locus_tag="ACHKAY_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702370.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKP"
BASE COUNT         3637 a         4245 c         4334 g         3291 t
ORIGIN      
        1 ccgtagtgga ccgacacgcg gtggccttca ctggtatggc tgagggtggt taaccacccg
       61 tgctcatcat actgccactg ctctgccggg tcgccgttca ccgtccggtg cgtgatgcgg
      121 tccgacgcat cgtagtgcca gagggtgata agcccctcgt cttcactctg tgtgagtttt
      181 ctggtcaggt catagtggta acgttgcgtc cggccgtcaa aaccgcgctg ttcagtcagc
      241 ctgtcaaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacggtg
      301 atgcgcccgg cagcgtcata ccccatgctg cgcgtcagac cgccttccgt cacactcacc
      361 ggacgcccgc gtttatcata tgcgatggtg ctgcgtttac cgtccggtga taccgttgct
      421 gtcaggtctc ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccttctga
      481 ctgaccagtt gcccacgcgg gttataactg ctgtaagtgc tgatgccttc ctcccggtga
      541 acggcgattt gttgaccgta ccggtcgtac tcataccgcg tcgtgtaccc cgagcagtcc
      601 gtaaaggcca gcagttgacc gtaacggctc catgccatct gttttgtact gcccgtcgcg
      661 tcctgtatcc ctgtcggcag ctcactcgcc ggccgctttc aggcattgcg gctgtccggc
      721 catcggcacc gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc
      781 tgccgcccat ttatgccagc cccggcgatc gtaatgacga cgtctctgcg gcagaaatgg
      841 cgcaacgcca ccttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga
      901 gcaattccga cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa
      961 taaaaggtct gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc
     1021 cgctggaaga taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt
     1081 ttgccgaagg taaaagcccg gtacatctgg cggctcccgc cgccgtcagt gaaagcctgc
     1141 gcaatgtccg ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc
     1201 tgctggatgc tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc
     1261 atgaactgcg ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg
     1321 aagaactggc ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc
     1381 aagtctatga ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc
     1441 gtttctggcc tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat
     1501 cagtgaggtg atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga
     1561 aataaccgtc accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc
     1621 cgccgtggat gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta
     1681 tgaccagata gccagtacgc cgctaccgga tgtgctgggt aaaaaatata tcgaccacca
     1741 ggatttcaac ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa
     1801 aacgcagttt attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca
     1861 aatcgagacg gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg tacgtcaaag
     1921 cagcatcgaa atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg
     1981 tggcaggaag ggatgctgat gcgtccacac catttccagc agcagcagcg ttacaacgac
     2041 tacctggata accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc
     2101 actctcaaca atgaactgct ggcacagggt aagatcatga ttgacagcgc gtcaggcaca
     2161 ctgcccgacg gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac
     2221 ccgcaacatt ttccggacga gagaagccgc aatatctacc tcgctctgcc ggtcgccagt
     2281 gatgtgagaa atgaaatcag cgacgggcgg cgaatcgggc gttaccggct gaattatgcc
     2341 gatgtccggg atttgcattc agaagaaggt gacacgcgaa cactgacgct gggacaactg
     2401 acgccgcgca tatgagcggt gcagaagata tgagcgccta tattacgctc ccgctttgcc
     2461 gtatcagcga tcgccatgcc gatggctccc tgacgttgga tgacgatttt atcccctcct
     2521 gccagaatat tcaggtcagt aagaaactgc gtgtttatct caaagaggta cagggggcca
     2581 ttggcggacg ggcaagcgat ctggcaaacc gtattggctc tccggcgcag agcggcatcg
     2641 cggatgtggc ggaatttatg atgttgcagt tacttaaccg taaccagacc cggtttaccc
     2701 atcgcgcccg ccgatcccag ctccacccgg aagatttcta ccttgatctt gctgggttgc
     2761 tgggtgaact gatgaccttt accgagccgt cgcgcctgcc ctgcccgctt gatgtgtatg
     2821 atcatcatga tctgaccaaa acatttaaaa cactgttacc ggaagtcaaa cgggcgctgc
     2881 ataccgtact gtcgccaaga gcggtcaatc tgccgctgca tctgcgtgac ggtatctggc
     2941 aggccgatat ccatgacaca gaactgctgc aatctgccac ctttgtgctg gccgtggcgg
     3001 caaacgtgcc ggtcgatcag atccagcgtc agtttatcca gcagtcgaaa atttcctcgc
     3061 cggaaaaaat ccgcaatatg gtcagtgtgc agatccccgg cattccattg cgcgcactga
     3121 tggtggctcc ccgccagctt ccttaccatt ccgggttcag ctatttcgaa ctcgacaaga
     3181 gcggacaggc ctggacagaa atggctgccg ccggggccgt tgcactgcat gtatccggca
     3241 gtttcccgga tctgaacatg caactgtggg cgataagagg gtaagacgat ggatgagggg
     3301 tcactgtctc tgccaccatt taccggatac gacgaaaaaa gccagcgcga ctatcatctc
     3361 gcgcttcgtg gcaacagcct gaatccgatg atcgacgcag ccacgccgct cctcggtatg
     3421 gtgatgcgcc tttcaaccat gaacagccag acgatgccgg agcatctttt tgctcaggtg
     3481 gtgacggatg ttcaggccgt ggagcaactg cttcaggagc agggctacga accgggcgtg
     3541 attatctcgt ttcgctacat cctctgcacg tttattgatg aagcggcgct cggtaacggc
     3601 tggtcgaaca agaacgagtg gatcaagcag tcattgctgg tccatttcca caacgaagcc
     3661 tggggtggag agaaagtttt catcctgctg gagcgcctga tccgtgaacc caaacgttac
     3721 caggatctgc tggaatttct ctggctctgt ttttctctgg gatttcgcgg gcgctacaaa
     3781 gtggcagcac aggatcaggg ggaatttgag cagatctacc gccgcctgta ccacgtcctg
     3841 cataaactac gcggcgatgc ccctttcccg ctgttacacc aggacaaaaa aacccagggc
     3901 gggcgttatc agctcatcag tcgcctgacc attaaacata ttttttgtgg tggcgttgtc
     3961 gtgctggcgt tgttttacct gttctatctg ttgcggctgg acagccagac gcaggacatc
     4021 ctgcaccagc taaacaaatt acttcgataa ggacgtttat gatccagatt gatcttccca
     4081 cgctggtaaa acggcttaac ctgttctccc gccaggcgct ggagatggcc gcctctgaat
     4141 gtatgagcca gcaggcagcg gaaattaccg tcagccatgt gcttattcag atgctcgcca
     4201 tgccacgcag tgacctgcgg gttattaccc gccagggcga tattggcatg gaagagttgc
     4261 gccaggcgct gacggtggag aactacacaa ctgcccgttc tgcggacagc tacccggcat
     4321 tttccccgat gctggttgag tggctgaaag agggctggct gctggcgtcg gctgagatgc
     4381 agcacagcga attgcgtggc ggcgtgttgc tgctggccct gctgcattcg ccgctgcgtt
     4441 atataccgcc tgctgccgcc cggctgttga ccggcattaa ccgtgaccgt ctgcaacagg
     4501 actttgtgca atggacacag gagtcggcgg aatcagtcgt gccggatgca gacggtaaag
     4561 gcgcaggcac actgacggac gctgctgaca ccctgcttgc ccgctacgcc aaaaacatga
     4621 ccgcagacgc ccgtaacggc aggcttgacc cggtactgtg ccgcgaccac gaaatcgacc
     4681 tgatgatcga cattctctgc cgccgccgca agaacaaccc ggtggtggtg ggcgaagcag
     4741 gcgtgggtaa aagcgcctta atcgaagggc tggcgctgcg catcgtggca ggccatgtgc
     4801 cggacaagct gaaaaacacc gatatcatga cccttgactt gggcgcattg caggccggag
     4861 cgtcggtgaa gggtgaattc gaaaaacgtt tcaaagggct gatggcagag gtcatttcct
     4921 ccccggtgcc ggtcattctg tttatcgacg aagcacatac cctgattggc gcgggcaacc
     4981 agcagggcgg gctggatatc tccaacctgc tcaaaccggc gctggcgcgc ggcgagctga
     5041 aaaccatcgc cgccaccacc tggagcgagt acaaaaaata cttcgaaaaa gatgccgccc
     5101 tgtcgcgccg cttccagttg gtgaaggtca gcgaacccaa cgctgccgaa gccaccatta
     5161 ttctgcgcgg tctgtcggcg gtctatgaac agtctcacgg cgtgctgatt gatgatgacg
     5221 ccttgcaggc cgctgcgaca ttaagcgagc gttatctctc cgggcgtcag ttaccggaca
     5281 aagcgattga tgtgctggat accgcctgcg cccgtgtggc catcaacctg tcgtcgccgc
     5341 cgaagcaaat ctcggcgctg accactctga gccaccagca ggaggcggaa attcgccagc
     5401 ttgagcgcga gcttcgcatc ggactgcgta ccgacacatc acggatgacc gaggtgctgg
     5461 tgcagtatga tgaaacgctg acggcgctgg atgaactgga agcggcctgg caccagcagc
     5521 agacgctggt ccgggagatt attgcgctgc gccagcagtt actgggcgtg gcagaggacg
     5581 atgcggcgcc gttgccggac gcagataccg tggaggatac gcagccagag tcagagtcag
     5641 agtcagagtc agagtcagag tcagagtcag agtcagagtc agagcaggat aataccggtg
     5701 ccgaaccggc tgatgaagct ggcagcgaac agccggaaga gaccgctgaa acagtttccc
     5761 cggtacagcg gctggcccag ctcactgccg aactggacgc cctgcataac gaccggttgc
     5821 tggtctcccc gcacgtcgat aaaaaacaga ttgcggcggt gattgccgaa tggaccggcg
     5881 tgccgctcaa ccgcctatcg cagaatgaaa tgtcggtcat caccgacctg acgaaatggc
     5941 tgggtgacac catcaaaggc caggacctgg cgattgccag cctgcataag catctactga
     6001 ccgcacgcgc cgacctgcgt cgtccgggac gcccgctcgg tgcgttcctg ctggctggcc
     6061 ccagcggtgt gggtaaaacc gaaaccgtcc tgcaactggc agagctgctc tacggcggtc
     6121 gccagtacct gaccaccatc aatatgtccg aattccagga gaaacacacc gtctcgcggc
     6181 tgattggttc gcctccgggc tacgttggct acggtgaagg cggcgtactg accgaagcga
     6241 ttcgccagaa accctactcg gtagtactgc tcgatgaagt ggaaaaagcg cacccggatg
     6301 tgctcaacct gttctaccag gcgttcgata agggcgaaat ggcagacggt gaaggccgcc
     6361 tgattgactg caaaaatatc gtcttcttcc tgacgtccaa cctcggctac caggtaatag
     6421 tcgagcatgc cgatgacccg gaaaccatgc aggaagtact gtatccggtg ctggccgact
     6481 tcttcaaacc tgccctgctg gcgcgtatgg aagtggtgcc gtacctgccg ctgtcgaaag
     6541 agacgctcgc caccatcatt gccgggaaac tggcccgcct ggataacgtg ctgcgcagcc
     6601 gctttggcgc ggaggtgatt atagaaccgg aagtgacgga cgaaatcatg agccgcgtca
     6661 cccgcgcgga aaacggcgcg aggatgctgg aatcggtcat cgacggcaat atgctgccgc
     6721 cgctctccct gctgctgttg cagaaaatgg cggcgaatac ggcggttgcc cggattcggt
     6781 tgtcggcagt ggacggcgca tttacggcag acgtggaaga tgctcagaac gacgagtccg
     6841 tcacaaagga tgaaacggtt ttatgagcag gttgcgcgtt ttccggcaga caggatggtt
     6901 tatcgcgggc ctgatgacgg gcttacccgc cactgccgcg cccgcagaag catcgtcaat
     6961 ggccggtgtg gcggtcgccg tcgccaccac gaccccgccg gatgcgacag ccacgttaca
     7021 ggccatgcag tcctgtcggc gggagtccgc ggcgctggag cggcttgact gctatgaccg
     7081 cctgctggca ccgctgtccc cgtcagggtt tgacggggcg ctggtcaaag ccggttttgt
     7141 gggcgaggcc tggacccgcg ccacagagca ggaaaaacgc cgcgagggca acaccacaga
     7201 actgctggtg acgcaggtgc cgggggagcg cccgacggtg gtgataacca ccccggctat
     7261 cggtcatgtt ccgccacgcc cggtgctgat gttcagttgc gtggacaata tcacccggat
     7321 gcaggtggcg ctgatgcacc ctctggatgt ccatgatatc gccgtcactc tgaatgccga
     7381 caaccgggca ttgcgcagcc actggtttgt gcgcgagaac ggcaccctgc tggagtccag
     7441 ccgggggtta tccgggattg atgaaattaa gcaactgttc ggcgcaaaaa cgctgacggt
     7501 ggataccggc gcagacaatg ccgccggaaa gctgactttt aacattgacg ggctggcaag
     7561 ggcgattgct cccctgcgcg atgcctgcca ctgggcggga gaataaaaat atggcaatgg
     7621 acttacgcga tccgaatgtc tggatatcgc acctgctgga aaacctgccg gaagagaaac
     7681 tggcatcggc gctgaaagat gacaacccga actgggagta tatcgacggc gaaatcgtca
     7741 agctgggatc gctggcccac agtcaactgg atatcccgga actccagcgc cgtgggctgg
     7801 tcattctggc ttcggagagt aaggattttc gtctgctggc gcatctgctg agaaccctgc
     7861 aacacgccgg tgatcctcat ctcgcattgc gcctgctggc gctgtatgtg gagcattact
     7921 gggcggtggc tgcgccgcag aatatggcgc ataaaaaacg ctttgccagc caggtcatta
     7981 aacgctttga aacaggtatt gaagtttttt cacagaacgc cgccacagcg cagcgcgatg
     8041 cgctgtcagg ggaactggca aaactggcgc aatgctggca gtcacataac gccccggaac
     8101 tggcacaggc tactgatgat ctttttgccc tgtaccagcg tgcgtttaat cgtgcggcac
     8161 cggctcctgt ccccactccg gcggcctccg gtagttcacc acagaccacc gccacgtctg
     8221 aaagcggcgt gacgcaaccc agtgctccgg ctccccaaat cgtcatcgac agtcacgacg
     8281 acaaagccta gcgcgacacg ctgttaaaag tggcggccat tttatgtgaa cgccagccgg
     8341 attcaccgca gggctaccgt ctgcgccgcc atgcgctttg gcagaacatc accagtacgc
     8401 cccaggcgga aagcgatggc cgtactccac tggctgcggt ctccgccgat atggtggcag
     8461 attaccatgc tcagctcggt agtgccgata tggcgctgtg gcagcaggtg gagaaaagcg
     8521 tactgctggc tccttactgg ctggatggtc actgcctttc tgcacagacg gcgctgcgtc
     8581 tgggttacaa acaggtggca gacgccatcc gcgatgaggt catccgcttc cttgagcgtc
     8641 tgccccagct taccgggctg ctgtttaatg accacacacc gtttatcagt gagcagacga
     8701 aacaatggct ggcagcttcg cccgacgcca aagttgcacc ggttgcgcaa atcggtgagg
     8761 aatcgaaggc agccagagcc tgttttgctg agcagggtct ggaggcggcg ctgcgatatc
     8821 tggacatgct acccgaaggc gatccccgcg atcagtttca ccgccagtac cttgccgcac
     8881 agttgacgga agaggcgggg ctggtacagc ttgcgcagca gcagtaccgg atgttgttca
     8941 ggatgggatt gcagatgatg gtggcagact gggagccgtc cctgcttgag cagctagaac
     9001 aaaaattcac ggcagaacaa taaaagagaa caggagtttt tgtgttcaga ttaccaacac
     9061 cccgactact cagcggactc aaatcagccc tgcgaccggc gatgccccgg tttaaagtct
     9121 ctgctttctg gctgctgata ctggcgtgga tttttctgct ggtgtggatc tggtggaaag
     9181 gcccgatgtg gacgctgtat gaagaacagt ggctcaaacc actggcgaac cgctggctgg
     9241 caacggcggc gtgggggatt attgccctgg tgtggcttac cgtccgggtg atgaagcgcc
     9301 tgcaacagct tgaaaaaatg caaaagcaac agcgcgagga agccgttgat ccgctcagcg
     9361 tggaactgaa cgcccagcag cgttatcttg accgctggct gctgcgcctg caacgccatc
     9421 tcgacaaccg ccgtttcctg tggcagttgc cgtggtatat ggtcatcggc ccggcgggca
     9481 gtggcaaaac gacactgctg cgcgaggggt ttccgtccga cattatttat gccccggagg
     9541 gcgcacgcgg cgcagaacaa cgcctgtacc tcacgcccca tgtcggtaaa caggcggtga
     9601 tctttgatat cgacggcaca ctctgcgctc ccgctgatgc ggatatcctg catcgccgtc
     9661 tgtgggaaca tgccctcggc tggctgaagg aaaagcgcgc acgccagccg ctgaacggga
     9721 ttattctgac actcgattta cccgatctgc tgaccgcaga caaacgccgc cgcgagcatc
     9781 tgttacagac gctgcgcagc cgcttacagg atatacgcca gcatcttcac tgccagttac
     9841 cggtttacgt ggtactgacc cggcttgatt tattacaggg ttttgccgcc ctgttccagt
     9901 cactcaacag acaggatcgc gatgcgattc tgggcgtcac gttcacccgc cgcgcccatg
     9961 aaaatgatga ctggcgaaca gagttgaatg ctttctggca gacatgggtg gatcgaatga
    10021 atctggcgtt gccggatctg atggtcgctc agactcacac ccgcgcgtct ttattcagtt
    10081 tttcccgcca gatgcaggga agccgtgaac cgctggtgtc actgcttgag ggtctgcttg
    10141 atggcgaaaa tatgaacgtg atgctgcgtg gtgtctatct cacctcttcg cttcagcgtg
    10201 ggcagatgga tgatatattc acccagtctg ccgcccgcca gtaccggctg ggcaataacc
    10261 cactggcgtc ctggcccctg gtggacaccg cgccttattt cacccgcagc ctgttcccgc
    10321 aggcattact cgcagagcct aatctggcaa cagagagccg cgcctggctg atacgttccc
    10381 gtcgccgcct gacggttttc tctgccacag gcggcgtggc agcactgctg ctcatcaccg
    10441 gctggcatca ctattacaac ggtaactatc agtccggcat caccgtgctt aagcaggcca
    10501 aagcctttat ggacgtgccg cctccgcagg gggaagatga ctttggcaac ctgcaactgc
    10561 cgctcctgaa cccggtacgc gatgccacac tggcctatgg cgactggggc gaccgcagcc
    10621 gtctggccga tatgggactg taccagggac gacgtatcgg gccttatgtg gaacagacct
    10681 atctgcaact gctggagcaa cgttacctgc cctcgctgtt taacgggctg gtcaaagcga
    10741 tgaacgccgc gccgccggag agtgaagaaa aactcgcggt gctgcgcgta atgcgaatgc
    10801 tggaggacaa aagcggacgt aacaatgagg tggtgaagca gtatatggca aaacgctgga
    10861 gcgaaaaatt ccacggccag cgcgatatcc aggcacaact gatgtcccat cttgactacg
    10921 cgctggctca tactgactgg catgcagagc gtcaggcggg cgacggtgac gccatcagcc
    10981 gctggacgcc atatgacaag cccgtggtat cagcacagaa agaactgagc aaactgcctg
    11041 tctaccagcg ggtttaccag agcctgaaaa cgcgggcgct gggcgttctt cctgccgacc
    11101 tcaatctgcg tgaccaggta gggccaacct ttgaccaggt gtttacatct gccgatgaca
    11161 acaaactggt tgttccacag tttatcaccc gctacggcct gcaaagctat tttgtaaaac
    11221 agcgcgatga actggttgaa ctgacagcga tggattcctg ggtacttaac ctcacccgca
    11281 atgtgaaata cagtgacgcc gaccgtgcgg aaatccagca ccagttgacc gagcagtata
    11341 tcagcgacta caccgccacc tggcgggctg ggatggacaa tctgaatatc cgcaattttg
    11401 agtccatcgg acaactgacc ggggcgctgg agcaggttat cagcggcgac cagcctttgc
    11461 agcgggcgct gaccgtgctg cgtgacaaca cacagccagg cgtcttttct gaaaaactct
    11521 ctgccaaaga acgggaggaa gccctggcag agccggatta ccagttactc acccgcctcg
    11581 ggcatgaatt cgccccggaa aacagtaccc tggcagtaca gaaagacaaa gaaagcacga
    11641 tgcaggccgt gtatcagcaa ctcaccgagt tgcaccgcta cctgctggca atccagaacg
    11701 cgcctgtacc agggaaatcg gcgctgaaag ccgtgcagtt acggcttgat cagaacagca
    11761 gcgatccgat attcgccacc cgccagatgg caaaaacgct gcctgctccg ctcaaccgct
    11821 gggttggcag gctggctgac caggcctggc atgtggtgat ggtggaggct gttcattata
    11881 tggaagtgga ctggcgcgac agcgtggtga aaccgtttaa cgagcaactg gcaaataact
    11941 atccgtttaa tccgcgttct gcacaggatg cctcactgga tgccttcgaa cgcttcttta
    12001 aaccggatgg catactggat accttctacc agcagaacct gaagctgttt atcgataatg
    12061 acctgagtct ggaggatggc gataacaacg tcattattcg cgaagatatt attgcgcaac
    12121 tggaaactgc gcagaaaatc cgtgacatct tcttcagcaa acagaacggt ctgggaacat
    12181 cctttgccgt ggaaacggta tcgctttcag gcaataaacg ccgcagtgta ctgaaccttg
    12241 acggtcagtt agtcgattac agccagggcc gtaactatac cgcccatctg gtctggccta
    12301 acaacatgcg cgaaggcaac gaaagtaagc tgacgctcat cggcaccagc ggcaacgcgc
    12361 cgcgcagtat cagcttcagc gggccgtggg cgcagttccg cctgttcggg gccggacaac
    12421 tgaccggagt acaggatggc aactttaccg tgcgctttag cgtggacggt ggcgcgatga
    12481 cctaccgtgt gcataccgac acggaagata acccgttcag cggtgggttg ttcagccagt
    12541 ttggtctgtc agacacactg tactgaggag gcagggatga atagtaacgt actgacacaa
    12601 actatcgtta ccggcagtga cccgcgcggg ctgccggaat tcagcgccat ccgcgaggaa
    12661 ataaacaaag ccagccaccc gtcacagcct gagctgaact ggaaactggt ggagtcgctg
    12721 gcgctggcga tttttaaagc caacggtgtg gatttacaca ccgccaccta ctatacgctt
    12781 gcccggacac ggacacaggg actggcggga ttctgcgaag gtgcggaact gctggcggca
    12841 atggtaagcc acgactggga taagttctgg ccgcagggcg gcccggcgcg tactgaaatg
    12901 ctggactggt ttaactcccg aaccggcaat attctgcgtc agcaaatctc ctttgcggaa
    12961 tccgacctac cactgatcta ccgcacagag cgggcattgc agcttatctg cgataagctc
    13021 cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc tgctctattt tatgcagaac
    13081 acgcgtaaac ggcttggacc ccagctgaag agtaacactg agaacgccgc acagaccacg
    13141 gtcagaacgc tgatttatgc cccggaaaca caggcatctt ccacaccaga agcggtagtg
    13201 cctcccctgc ccggcctgcc tgagatgaaa gtggaagttc gcagtctgac agagaatccc
    13261 ccacaggcca gtgtgataaa tcaaggcagt acggtaagag ggtttatcgc agggatcgcc
    13321 tgttcagtgg ctgtcgcctc agcattgtgg tggtggcagg tctatccggt gcagcagcaa
    13381 ctgttacagg ttaacgacac cgctcagggc gcagcaacgg tgtggatggc ctcacctgaa
    13441 ctcgaaaact atgagcgcag gctgcaacaa cttcttgata cctccccggt acagccgctg
    13501 gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct ggccggaaag cctgcaacag
    13561 caacaggcct cgacacaatg gaatgaggca ctcaaaaccc gcgcacagag tagcccgcag
    13621 ttgcgtggct ggttgcagac ccgccaggac ttacatgctt ttgcagatct agtgatgcag
    13681 cgcgagaaag agggactaac cctttcctat atcaaaaatg tcatctggca ggcggagcgg
    13741 ggactggggc aggaaacacc cgttgagtct ctgttgacgc agtaccatga tgcccgtgcg
    13801 cagaagcaga atacagatgc gctggaaaaa caaattaatg agcgactcga aggcgtgtta
    13861 agccgctggc tgctgctgaa gaataacgtc atgccagagg cggcaaccgg taccacggcc
    13921 gaaaaataat tatttaatta aaggagatta tcatggcaaa tataagttat ttatctttga
    13981 gtggagagac acaagggcta atttcagcag gttgctcaac gcttgattct gtcggaaaca
    14041 aagcgcagcc agaacataaa gatcagatca tggtttatgc gttaatgcat tccatctcaa
    14101 gaagtcaaaa tgtcaatcat catgaattaa ttatcaccaa accggttgat aaatcctcac
    14161 cattgctggc aaaagcactt agtgacaatg aaaaaatggc aatatgtgaa tttatactct
    14221 accgaaccag taaagcgggt atttaccagc cttattataa aataaatctg tcaaaagccc
    14281 gaatatcaag tattgatttt gtcacaccgc atgctgtcct ggaaaaagaa ctggaaccac
    14341 aggagagaat tgcctttatt tatgaagata tttcctggga gcatacgtta gccggtacga
    14401 atgcaatgag taaatggcag gatcgtgtac agtaacttac tgtaataaca taataaatta
    14461 ctattgcgga gagataaaat ggctggttta aggaataaca acaacacaca gaatgcacag
    14521 tgggctgatt atgtgggtga cattcttcgg ggggctcagc cgataaacca gctagtacct
    14581 cagcaccctt atcttaacga cgtcccactc attgatgagt taaggcatca gaacactcat
    14641 catgttattc tcttgacact tgatgttgca aaaaaaatac tcagcccaat aacatcattt
    14701 gattacattc attttatcac tacacatcct tcaggtataa aagatacact cgcctggtta
    14761 gttaacgcgg gtaaattaat gactgagttt gatgacaatg ggaaaattat atttaacctt
    14821 aatgccttaa agtatacaaa agcaagttac tttgaaatac ttggagagaa atatataaaa
    14881 atcacaacat ccagcccctg gcttttagaa aagctcggta aatatatttt ctcttcacga
    14941 gcgcctcaag ttctggaact cgctattggc tggcgaggag ctttatctga atcaataaaa
    15001 ggagttaagt tctgtatctg gttttcagta gcctggagaa ccatagaatt tattatgagt
    15061 tcagagcgag atctggttaa ttttcttggc gatttttcaa tggatgtggc caaagcagtt
    15121 atagccggtg gtgttgcaac cgctattgga agcctggctt cttttgcctg tgttagcttt
    15181 ggctttccag taattcttgt cggaggagca attttactga cagggatagt gtgtacagtt
    15241 gttttaaatg aaatcgatgc tcaatgccat ttatcagaaa aattaaaata tgcaattaga
    15301 gatggactaa aacggcaaca ggaacttgat aaatggaaaa gggaaaacat gactccattt
    15361 atgtatgttc ttaacactcc acccgtgata tgaggattaa ccatgcgttt acatgttaaa
    15421 ttaaaagagt ttctttcgat gttttttatg gccatattat ttttccctgc attcaatgct
    15481 tctttgtttt ttaccggagt taaacct
//
LOCUS       DAZFKM010000116        15460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFKM010000116 DAZFKM010000000
VERSION     DAZFKM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(324..1355)
                     /gene="rsmC"
                     /locus_tag="ACHKAY_003778"
     CDS             complement(324..1355)
                     /gene="rsmC"
                     /locus_tag="ACHKAY_003778"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO5702371.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1458..1871
                     /gene="holD"
                     /locus_tag="ACHKAY_003779"
     CDS             1458..1871
                     /gene="holD"
                     /locus_tag="ACHKAY_003779"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO5702372.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            1840..2286
                     /gene="rimI"
                     /locus_tag="ACHKAY_003780"
     CDS             1840..2286
                     /gene="rimI"
                     /locus_tag="ACHKAY_003780"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO5702373.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2301..2978
                     /gene="yjjG"
                     /locus_tag="ACHKAY_003781"
     CDS             2301..2978
                     /gene="yjjG"
                     /locus_tag="ACHKAY_003781"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO5702374.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3069..4658
                     /gene="prfC"
                     /locus_tag="ACHKAY_003782"
     CDS             3069..4658
                     /gene="prfC"
                     /locus_tag="ACHKAY_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO5702375.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5051..5656
                     /gene="osmY"
                     /locus_tag="ACHKAY_003783"
     CDS             5051..5656
                     /gene="osmY"
                     /locus_tag="ACHKAY_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO5702376.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5783..5944
                     /locus_tag="ACHKAY_003784"
     CDS             5783..5944
                     /locus_tag="ACHKAY_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO5702377.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6066..7139
                     /locus_tag="ACHKAY_003785"
     CDS             6066..7139
                     /locus_tag="ACHKAY_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO5702378.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7136..7918
                     /locus_tag="ACHKAY_003786"
     CDS             7136..7918
                     /locus_tag="ACHKAY_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5702379.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8435..9298)
                     /locus_tag="ACHKAY_003787"
     CDS             complement(8435..9298)
                     /locus_tag="ACHKAY_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO5702380.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(9270..10820)
                     /locus_tag="ACHKAY_003788"
     CDS             complement(9270..10820)
                     /locus_tag="ACHKAY_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO5702381.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            11078..11857
                     /gene="deoC"
                     /locus_tag="ACHKAY_003789"
     CDS             11078..11857
                     /gene="deoC"
                     /locus_tag="ACHKAY_003789"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO5702382.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11984..13306
                     /gene="deoA"
                     /locus_tag="ACHKAY_003790"
     CDS             11984..13306
                     /gene="deoA"
                     /locus_tag="ACHKAY_003790"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO5702383.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13358..14581
                     /gene="deoB"
                     /locus_tag="ACHKAY_003791"
     CDS             13358..14581
                     /gene="deoB"
                     /locus_tag="ACHKAY_003791"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO5702384.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14661..15380
                     /gene="deoD"
                     /locus_tag="ACHKAY_003792"
     CDS             14661..15380
                     /gene="deoD"
                     /locus_tag="ACHKAY_003792"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO5702385.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3633 a         4059 c         4212 g         3556 t
ORIGIN      
        1 tctaccaatt ccgccacctt cgcataccat caattcttaa aaagaattgc taccacggag
       61 gcgcattcta gtggttttca gcttttcgtc aatagttaat tatcgccgga ggtgtaattg
      121 ctggaaaaat gtccatcagg aaactaacgt gcaggttttg taggtcggat aaggcgttca
      181 cgccgcatcc gacacggtat ttgggtgcag atgccagatg cgacgcgggc gcgtcttatc
      241 tggcctacga agggctaacg tgcaggtttt gtaggttgta taaggcgagc acgccgcatc
      301 cgacaaggct tgcggcggga tacttaacct ttcttcgcct ggcgggtcat aatggcgcga
      361 tacaccttga agcgcccggt ttgcgcgata acttcatgga agccaaatgt ctcatccagc
      421 acgtccgggt aaggcaggaa ggcgttcgct acaattcgca gctcgccgcc gctattaaga
      481 tgacgcaccg cgccgcgaat cagcgtttgc gccgcatcca ggctggtttg catcccatcg
      541 tggaacggcg ggttggagat gatcatatca aaacgacctt tcacctcgga aaagacgttg
      601 ctggcaaaga cttcaccttc aacaccgttg gccgcaagcg tcgcacggct ggcttctacc
      661 gccggggcag agacatcgca caaggtgaga cgaattttcg gcgaatggcg cgcaaaggca
      721 actgaaagca cacccgcgcc acagccgaca tccagcactt tacctttcgt gtgcggggtt
      781 aacgtcgaga gcagcaactg gctaccgaca tccagaccgt cgcggctaaa cacgccaggc
      841 agcgttttga ccgtcaggcc atcgacgctg tattcgcccc agaatttatc cgcatcaaat
      901 actggctgtt tttccagacg accaaaatag aggccacagc gacgagcgct gtcgacttta
      961 ttcaacggcg cataatctgc cagcatctgc tcggcgctgc gcacgccgct gcggttctcg
     1021 ccaacgacaa aaatatccgt ccccaccggc agcagagaaa gtaaattcat caactggaac
     1081 tgggcttccg gtttgttctt cggccagtag taaatcagcg tatcgcaatc tgcgacgtca
     1141 tccgccttgg cgaccagact aaaacgggcg ttatccccca tctggcggct taatacctgc
     1201 cagtggtgga attgctgggt atgagcacgg ctggccgcgg tatctaaacg cgcgggcagg
     1261 tcatcctgta agtctccggc aaacagaata cggctttgtt cgaaatcatc actgtgacgc
     1321 agcaagactt cacttgccgg ggtaaatgca gacatggaat gctcctcaat tgatactggc
     1381 ggcgattata gccatatgtt ggcgcggtat cgacgaattt gctatatttg cgcccctgac
     1441 aacaggagcg attcgctatg acatcccgac gagactggca gttacagcaa ctgggcatta
     1501 cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga aattgccatt gcgatcccgg
     1561 cacacgtccg tctggtgatg gtggcaaacg atcttcccgc cctgactgat cctttagtga
     1621 gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt gctgcaactg acgccagaaa
     1681 aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg gcggttgggt actgacgaac
     1741 cgctatcact ggaaggcgct caggtggcat caccggcgct caccgattta cgggcaaacc
     1801 caacggcacg cgccgcgtta tggcaacaaa tttgcacata tgaacacgat ttcttccctc
     1861 gaaacgactg atttaccagc ggcttaccac attgaacaac gcgcccacgc ctttccgtgg
     1921 agtgaaaaaa cctttgccag caaccagggc gagcgttatc tcaactttca gttaacgcaa
     1981 aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc tggatgaagc tacattgttc
     2041 aatattgcgg tcgatcctga ctatcagcgt cagggattgg gaagggcgct gctggaacat
     2101 ctgatcgacg aactggaaaa acgcggcgtg gcgacgctct ggctggaagt ccgtgcctca
     2161 aatgctgccg ccattgccct gtacgaaagt ttaggcttta acgaggcgac gattcgccgc
     2221 aattactacc ccaccacgga cggtcgcgaa gacgccatca tcatggcgtt gccaatcagt
     2281 atgtaagaca aggtggaata atgaagtggg actggatttt ctttgatgcc gatgaaacgc
     2341 tgtttacctt tgactcattc accggcctgc agcggatgtt tcttgattac agcgtcacct
     2401 ttaccgctga agattttcag gactatcagg ccgttaacaa gccactgtgg gtggattatc
     2461 aaaacggcgc gatcacttca ttacagcttc agcacgggcg tttcgagagc tgggccgaac
     2521 ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat taatgcgatg gcggaaatct
     2581 gcacgccgct gccgggcgcg gtttctctgc ttaacgccat tcgtggcaac gccaaaatcg
     2641 gcatcatcac caacggcttt agcgccttgc agcaagtgcg tctggaacgc acgggcctgc
     2701 gtgattactt cgatttgctg gtgatttccg aagaagttgg cgttgctaaa ccgaataaga
     2761 aaattttcga ttatgcgctg gaacaggcgg gcaatcctga ccgttcacgc gtgctgatgg
     2821 ttggcgacac tgccgagtcc gatattctcg gtggcatcaa cgccgggctt gcgacctgct
     2881 ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc gcccacctgg accgtttctt
     2941 cgttgcacga actggagcag ctcctgtgta aacactgatt gcctcccccc cgttgatggg
     3001 taaaatagcc gcaatttttc gttttcaaca agcgcggcgc gatgccgctt actcaagaag
     3061 aaagaattat gacgttgtct ccttatttgc aagaggtggc taagcgccgc acttttgcca
     3121 ttatttctca cccggacgcc ggtaagacta ccatcaccga gaaggtgctg ctgttcggac
     3181 aggccattca gaccgccggt acagtaaaag gccgtggttc caaccagcac gctaagtcgg
     3241 actggatgga gatggaaaag cagcgtggga tctccattac tacgtctgtg atgcagtttc
     3301 cgtatcacga ttgcctggtt aacctgctag acaccccggg gcacgaagac ttctcggaag
     3361 atacctatcg taccctgacg gcggtggact gctgcctgat ggttatcgac gccgcaaaag
     3421 gtgttgaaga tcgtacccgt aagctgatgg aagttacccg tctgcgcgac acgccgatcc
     3481 tcacctttat gaacaaactt gaccgtgata tccgcgaccc gatggaactg ctcgatgaag
     3541 ttgagaacga gctgaaaatc ggctgcgcac cgatcacctg gccgattggc tgcggcaagc
     3601 tgtttaaagg cgtttaccac ctttataaag acgaaaccta tctctatcag agcggtaaag
     3661 gtcacaccat tcaggaagtc cgcattgtta aagggctgaa taacccggat ctcgatgctg
     3721 cggttggtga agatctggcg cagcagctgc gtgacgaact ggaactggtg aaaggcgcgt
     3781 ctaacgagtt cgacaaagaa ttgttccttg cgggcgaaat cactccggta ttcttcggta
     3841 ctgcgctggg taacttcggt gtcgatcata tgctggatgg cctggtggag tgggctcctg
     3901 cgccgatgcc gcgtcagact gatacccgta ccgtagaagc gagcgaagac aaatttaccg
     3961 gcttcgtatt taaaattcag gccaacatgg acccgaaaca ccgcgaccgc gtggcgttta
     4021 tgcgcgtggt atccggtaaa tatgaaaaag gcatgaagct gcgccaggtg cgcaccgcga
     4081 aagacgtggt gatctctgac gcgctgacct ttatggcggg tgaccgttcg cacgttgaag
     4141 aagcgtatcc tggcgatatc ctcggcctgc acaaccacgg caccattcag atcggcgaca
     4201 cctttaccca gggtgagatg atgaagttca ccggtattcc gaacttcgca ccagaactgt
     4261 tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca gctgctcaaa gggctggtac
     4321 agctttccga agagggcgcg gtgcaggtgt tccgtccgat ctccaacaac gatctgatcg
     4381 ttggtgcagt tggtgtgctg cagtttgatg tagtggtatc gcgcctgaag agcgaataca
     4441 acgttgaagc agtatatgaa tcagtcaacg ttgccaccgc ccgctgggta gaatgtgcag
     4501 acgcgaagaa attcgaagag ttcaagcgta agaacgaaag ccaactggcg cttgatggcg
     4561 gcgataacct cgcttacatc gctaccagca tggtcaacct gcgcctggcg caggaacgtt
     4621 atccggacgt tcagttccac cagacccgcg agcattaatt cttgcctcca gggcgcggta
     4681 gccgctgcgc cctgtcaatt tcccttcctt attagccgct tacggaatgt tcttaaaaca
     4741 ttcacttttg cttatgtttt cgctgatatc ccgagcggtt tcaaaattgt gatctatatt
     4801 taacaaagta atgacatttc tgacggcgtt aaataccgtt caatgcgtag atatcagtat
     4861 ctaaagccgt cgattgtcat tctaccgata ttaataactg attcagaggc tgtaatggtc
     4921 gttattcatc acccatcgct tttgtgatgg cgaccattga cttctgtaga gggtgaagtc
     4981 tctccctatt cagcaatgca acctcgtgtt gccaggctca aattacgagc aaacatacag
     5041 gaataaatcg atgactatga caagactgaa gatttcgaaa actctgctgg ctgtaatgtt
     5101 gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac aacgcacaga ctaccaatga
     5161 aagcgcaggg caaaaagtcg atagctctat gaataaagtc ggtaatttca tggatgacag
     5221 cgccatcacc gcgaaagtga aggcggcgct ggtggatcat gacaacatca agagcaccga
     5281 tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc ggtttcgttg aaagccaggc
     5341 tcaggccgaa gaggcagtga aagtggcgaa aggcgttgaa ggggtgacct ctgtcagcga
     5401 caaactgcac gttcgcgacg ctaaagaagg ctctgtgaag ggctacgcgg gtgacaccgc
     5461 caccaccagt gaaatcaaag ccaaactgct ggcggacgat atcgtccctt cccgtcatgt
     5521 gaaagttgaa accaccgacg gcgtggttca gctctccggt accgtcgatt cccaggcaca
     5581 gagcgaccgt gctgaaagta tcgccaaagc ggtagatggt gtgaaaagcg ttaaaaatga
     5641 tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa ctttgtcggg aggcgcgatg
     5701 tgcaccacac taaaaatatc gcattgagtg attttcacgc tcatatcaag cggtgacatt
     5761 aactatggta aaggagacgc ttatgtttcg ttggggcatc atatttctgg ttatcgcgtt
     5821 aatcgccgcc gcacttgggt ttggtggtct ggccggtacc gctgcaggcg cagctaaaat
     5881 tgtctttgtc gtcgggatta ttctgttcct ggtgagtttg ttcatgggcc gaaaacgacc
     5941 ctagatttca gaccattaag aaaattttat ccaaagccag tccagcggac tggcttttgc
     6001 ggttttagcg aataataaat tgcgttactt tgtcattctt tcacaacaaa aacaggaagg
     6061 cagaggtggg gcagcgaata cctgtaacgc ttggtaatat tgcgccgttg tcgctaaggc
     6121 cgttccagcc tggacgaata gctctggtgt gcgaaggcgg cggacagcgt ggaattttca
     6181 cggctggcgt gctggatgag tttatgcgcg cgcagtttaa tcctttcgat ctttatctcg
     6241 gcacatctgc cggggcgcag aacctctcgg cgtttatctg caatcagccc ggttacgcgc
     6301 gcaaagtcat catgcgctat accacaaaac gcgaattttt cgatccattg cgctttgtcc
     6361 gtggaggaaa tcttatcgat ctcgactggc tggtggaggc caccgcaagc cagatgccgt
     6421 tgcaaatgga taccgccgcg cggttgtttg acagcggcaa atcgttttat atgtgcgcct
     6481 gtcgtcagga tgactacgcg cctaattact ttttaccaac caaacaaaac tggctggatg
     6541 tgattcgcgc ctccagtgcg atacctggct tttatcgtag cggagtgtcg ctggaaggca
     6601 ttaactacct ggatggcggg atcagtgatg cgattccggt taaagaggcg gcaaggcagg
     6661 gcgctaaaac gttggtcgtc atccgcactg tgccgtcaca aatgtactac acgccgcagt
     6721 ggttcaaacg catggaacgc tggctgggtg acagtagcct gcagccgctg gtcaatctgg
     6781 tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt tattgagaaa ccaccgggca
     6841 agctgcggat attcgaaatt tatccaccga aaccattaca tagtatcgcg cttggcagtc
     6901 ggattccggc gctgcgtgaa gactataaac ttgggcgttt atgcggtcgt tatttcctcg
     6961 ccacggttgg caagctatta actgaaaaag cgccgcttac ccgccatctg gtgccagtgg
     7021 tgacgccgga atcgatcgtt attccgcctg cgccagtcgc caacgatacg ctggttgccg
     7081 aagtgagcga tgctccgcag gcgaacgacc cgacatttaa caatgaggat ctggcttgat
     7141 ttgccgtttt atcgataccc actgccattt tgatttcccg ccgtttagtg gcgatgaaga
     7201 ggccagcctg caacgcgcgg cacaagcggg cgtaggcaag atcattgttc cggcgacaga
     7261 ggcggaaaat tttgcccgcg tgcaggcgtt agcggaaaaa tatcaaccgc tgtatgccgc
     7321 attggggttg catccgggga tgttggaaaa acatagcgat gtgtctcttg atcagctaca
     7381 gcaggcgctg gaaaggcgtc cggcgaaggt ggtggcggtg ggggagatcg gtctggatct
     7441 cttcggcgac gatccgcaat ttgagaggca gcagtggtta ctcgacgaac agctgaaact
     7501 ggcgaaacgc tacgatctgc cggtgatcct tcattcccgg cgtacacacg acaaactggc
     7561 gatgcatctt aaacgtcacg atttaccgtg cactggcgtg gttcacggtt tttccggcag
     7621 cctgcaacag gccgagcggt ttgtacagct gggctacaaa atcggcgtag gcggtactat
     7681 cacctatcca cgcgccagta aaacccgcga tgtcatcgca aaattaccgc tggcatcgtt
     7741 gttgctggaa accgacgcgc cggatatgcc gctcaacggc tttcaggggc agcctaaccg
     7801 accggaacag gctgtgcggg tgtttgacgt gctttgcgag ttgcgcccgg aaccagaaga
     7861 tgagattgcc gaagtgttgc ttaataatac gtatgcgttg tttagcgtta gtgggtagtt
     7921 tacccttcgt aggcagcatc cggcagtcgg cgcataatgc ctgatgcgac gcttgtcgcg
     7981 tcttatcatg cctacaggtt tgtgccgaac cgtaggccgg ataaggcgtt cacgccgcat
     8041 ccggcagttg gcgcacaatg cctgatgcga cgcttgtcgc gtcttatcat gcctacaggt
     8101 ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca tccggcagtc ggcgcataat
     8161 gcctgatgcg acgcttgtcg cgtcttatca tgcctacagg tttgtgccga accgtaggcc
     8221 ggataaggcg ttcacgccgc atccggcagt tggcgcacaa tgcctgatgc gacgcttgac
     8281 gcgtcttatc atgcctacaa gtctgtgccg aaccgtaggc cggataaggc gttcacgccg
     8341 catccggcag tcggcgcata atgcctgatg cgacgcttgt cgcgtcttat catgcctaca
     8401 agtctgtgcc gaaccgtagg ccgtatccgg catgtcacaa atagagcgcc ggaaatatca
     8461 accggctcac cccgcgcacc tttaacgcat cagccaacgg ctcaacgtct tccggcgtgg
     8521 cgctcgccca gctttgcgcc tcgccataca cgccgtgggc atgaaacgcg ttcaggcgta
     8581 ccggaacatc gccaagtccc ttgataaaca ccgccagttc ttcgatgtgt tgcaaataat
     8641 ccacctggcc agggattacc agcaaacgca gttccgccag cttaccgcgc tctgccagca
     8701 aacagatgct gcgcttaatc tgctgattat cgcgtccggt taattgttga tgacattcgc
     8761 tcccccacgc tttgagatcg agcattgcgc cgtcacacac cgggagtaat ttttcccagc
     8821 cggtttcgct caacataccg ttactgtcca cgaggcaggt gaggtggcgc agttgcggat
     8881 cgtttttgat ggctgtaaac agcgccacca caaacggcag ctgggtcgtg gcttcgccgc
     8941 cgcttaccgt tatcccttcg ataaacagca ccgctttgcg gatatggcta agcacctcat
     9001 ccacgctcat ggattgcgcc attggcgtgg catgttgcgg acacatcttc aggcaggtat
     9061 cacactgctc gcagaccaca gcgttccaca gcactttgcc gtcaacaatc tgcaacgcct
     9121 gatgcggaca ctgtggcacg cactctccgc aatcattgca gcgtcccatc gtccacggat
     9181 tgtgacagtt tttgcagcgc agattgcagc cctgcaaaaa cagagccaga cgactgcctg
     9241 gcccgtcaac gcaggagaag gggataatct tactgactaa agcgcatctg ctgttcatgg
     9301 cttatcacgc gcggctggcg ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc
     9361 caggtggtgt tggtgcgtga accttcggcg cgatattttt ctaaatccga caaacgcacc
     9421 atataaccgg taacgcgaac cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt
     9481 ccggctttaa aggcaccgag gcaaagctgt accagtgcct gcgggttacg tttgatggtt
     9541 tcgtcgaggg tcagaatgtc gctgatgccg gaataataat aagcatgatg cggggcgaca
     9601 gtttgcagat gggtgatcgg atctggctca tcgccatacg gcaaacgcgc gcccggcgtg
     9661 gtgccgatat cggaactgat ccccgactgt gcgtgtaaca tggcgcgttt ttgccagcca
     9721 tatttcacgg gggtattggc gacaaactcc gccagttgcg cgctgatgcg ataacccact
     9781 tcatttgcgg cggcttcttt accgtagcgc gcggcaatcc cttctttctc acacagcaag
     9841 ttaaccgctt ccgccagccc atacatgcca aacattggca caaaacgttc agggttaatc
     9901 agcccttctt tcaccaggaa gctattctca aagaagtgtg attgttgata gaggaattca
     9961 caccgcgcat cgatgatggc gatctgctgc tggcagtagt gcggtagagt gcgcgtaaag
    10021 aagtcatcca gcgattcgct gcgctcggca atggctttca ggttaaggcg taccagcgtg
    10081 ctgccaccac ccgccagcgg cagtgagttg taacagctca caatcccgta gccccctttt
    10141 gtgaaaattt tatcatgcac cggaccgttg gcgatgtgcg gtttgctaca ttcacagatg
    10201 ttcttcgcca cttccagcag caggtcatca ggggtgattt ctggatcgta gataaaggtc
    10261 aggttcggtg aaacctgctt caactctgca tctgcacgta atatcgcacg ggtgatgggc
    10321 gaatcagacg ggccgatatt ggcgtgcata aaggcgtctg gcagggttct gtcgaggtaa
    10381 cgccagaaac gttttattcg aatatcgatc tcgtcttgtg ttagaattct aacatacggt
    10441 tgcaacaacg catccagttg ccccaggtag accggcatcg atgtgaccga cggtacgtgg
    10501 tggtaaagaa tggtcagcag agagagtgcg tcatcaagat ctttcgcgcc ttccagctcc
    10561 agccattcgg aaccgttcgc cagaaaacgg gcgtaatcgg gtaagacata gcgcggtttg
    10621 tacggcgcat gaccttcaaa catatcgcag attacaccct catccagcgc gcggcgggcc
    10681 tcggcaggca gctgcggata aggcagattg ttttctgctt ccagtgccag aaaatggcgc
    10741 ttctgctccg ggctaagcac tgggctggtg acaatttgct ggcaacgttg ttgcagtgca
    10801 ttttcatgag aagtgggcat cttcttttcc ttttatgccg aaggtgatgc gccattgtaa
    10861 gaagtttcgt gatgttcact ttgatcctga tgcgtttgcc accactgacg cattcatttg
    10921 aaagtgaatt atttgaacca gatcgcatta cagtgatgca aacttgtaag tagatttcct
    10981 taattgtgat gtatatcgaa gtgtgttgcg gagtagatgt tagaatacta acaaactcgc
    11041 aaggtgaatt ttattggcga caagccagga gaatgaaatg actgatctga aagcaagcag
    11101 cctgcgtgca ctgaaattaa tggacctgac caccctgaat gacgacgaca ccgacgagaa
    11161 agtaattgct ctgtgtcatc aggccaaaac cccggtcggc aataccgccg ctatctgtat
    11221 ctatcctcgc tttatcccga ttgctcgcaa aacgctgaaa gagcagggca ccccggaaat
    11281 ccgaatcgct acggtaacca acttcccaca cggtaacgat gacatcgaaa tcgcgctggc
    11341 agaaacccgt gcggcaatcg cctacggtgc agatgaagtt gacgttgtgt tcccgtaccg
    11401 cgcgctgatg gcgggcaacg agcaggttgg ttttgacctg gtgaaagcct gtaaagaggc
    11461 ttgcgcggca gcgaatgtac tgctgaaagt gatcatcgaa accggcgaac tgaaagacga
    11521 agcgctgatc cgtaaagcgt ctgaaatctc catcaaagcg ggtgcggact tcatcaaaac
    11581 ctctaccggt aaagtggctg tgaacgcgac gccggaaagc gcgcgcatca tgatggaagt
    11641 gatccgtgat atgggcgtag aaaaaaccgt tggtttcaaa ccggcgggcg gcgtgcgtac
    11701 tgcggaagat gcgcagaaat atctcgccat cgcagatgaa ctgtttggtg ctgactgggc
    11761 tgatgcgcgt cactaccgct ttggtgcttc cagcctgctg gcaagcctgc tgaaagcgct
    11821 gggtcacggc gacggtaaga gcgccagcag ctactaagta agatgcttta cgcctgatgc
    11881 gctgcgctta tcaggcctac gagacgtatc tacccgtagg ccggataagg cgtagacgca
    11941 tccggcaaaa gccgcctcat actcttttcc tcgggaggtt accttgtttc tcgcacaaga
    12001 aattattcgt aaaaaacgtg atggtcatgc gctgagcgat gaagaaattc gtttctttat
    12061 caacggtatt cgcgacaaca ctatctccga agggcagatt gccgccctcg cgatgaccat
    12121 tttcttccac gatatgacaa tgcctgagcg tgtctcgctg accatggcga tgcgagattc
    12181 aggaaccgtt ctcgactgga aaagcctgca tctgaatggc ccgattgttg ataaacactc
    12241 gaccggcggc gtcggcgatg tgacttcgct gatgttgggg ccgatggtcg cagcctgcgg
    12301 cggctatatt ccgatgatct ctggtcgcgg cctcggtcat actggcggta cgctcgacaa
    12361 actggaatcc atccctggct tcgacatttt cccggatgac aaccgtttcc gcgaaattat
    12421 taaagacgtc ggcgtggcga ttatcggtca gaccagctca ctggctccgg cggataaacg
    12481 tttctacgcg acccgtgata ttaccgcaac cgtggactct atcccgctga tcactgcctc
    12541 gatcctggcg aagaaactgg cggaaggtct ggatgcgctg gtgatggacg tgaaagtggg
    12601 tagcggcgcg tttatgccga cctacgaact ctctgaagcc cttgccgaag cgattgttgg
    12661 cgtggctaac ggcgctggcg tgcgcaccac cgcgctgctc accgacatga atcaggtact
    12721 ggcctccagt gcaggtaacg cggttgaagt tcgtgaagcg gtgcagttcc tgacgggtga
    12781 gtatcgtaac ccgcgtctgt ttgatgtcac gatggcgctg tgcgtggaga tgctgatctc
    12841 cggcaaactg gcgaaagatg acgccgaagc gcgcgcgaaa ttgcaggcgg tgctggacaa
    12901 cggtaaagcg gcagaagtct ttggtcgtat ggtagcggca caaaaaggcc cgaccgactt
    12961 cgttgagaac tacgcgaagt atctgccgac agcgatgctg acgaaagcag tctatgctga
    13021 taccgaaggt tttgtcagtg aaatggatac ccgcgcgctg gggatggcag tggttgcaat
    13081 gggcggtgga cgccgtcagg catctgacac catcgattac agcgtcggct ttactgatat
    13141 ggcgcgtctg ggcgaccagg tagacggtca gcgtccgctg gcggttatcc acgcgaaaga
    13201 cgaaaacagc tggcaggaag cggcgaaagc ggtgaaagcg gcaattaaac ttgccgataa
    13261 agcaccggaa agcacaccaa ctgtctatcg tcgtatcagt gaataacggt atactgatct
    13321 gatcatttaa atttgaagca ctgagtacgg agaacatatg aaacgtgcat ttattatggt
    13381 gctggactca ttcggcatcg gcgctacaga agatgcagaa cgctttggtg acgtcggggc
    13441 tgacaccctg ggtcatatcg cagaagcctg tgccaaaggc gaagctgata acggtcgtaa
    13501 aggcccgctc aatctgccaa atctgacccg tctggggctg gcgaaagcac acgaaggttc
    13561 taccggtttc attccggcgg gaatggacgg caacgctgaa gttatcggcg cgtacgcatg
    13621 ggcgcacgaa atgtcatccg gtaaagatac cccgtctggt cactgggaaa tcgccggtgt
    13681 cccggttctg tttgagtggg gatatttctc tgatcacgaa aacagcttcc cgcaagagct
    13741 gctggataaa ctggtcgaac gcgctaatct gccgggttac ctcggtaact gccactcttc
    13801 cggtacggtc attctggatc agctgggcga agagcacatg aaaaccggca agccgatttt
    13861 ctatacctcc gctgactctg tgttccagat tgcctgccac gaagaaacct ttggcctgga
    13921 taaactctac gaactgtgcg aaatcgcccg tgaagagctg accaacggcg gctacaacat
    13981 cggtcgtgtt atcgctcgtc cgtttatcgg tgacaaagcc ggtaacttcc agcgtaccgg
    14041 taaccgtcac gacctggctg ttgagccgcc agcaccgacc gtgctgcaga aactggttga
    14101 tgaaaaacac ggtcaggtgg tttctgtcgg taaaattgcc gacatctacg ccaactgcgg
    14161 tatcaccaag aaagtgaaag caactggcct ggacgcgctg tttgacgcca ccatcaaaga
    14221 gatgaaagaa gcgggtgata acactatcgt cttcaccaac ttcgttgact tcgactcttc
    14281 ttggggccac cgtcgcgacg tcgccggtta tgctgcgggt ctggagctgt tcgaccgccg
    14341 tctgccggag ctgatgtctc tgctgcgcga tgacgacatc ctgatcctca ctgctgacca
    14401 cggttgtgat ccgacctgga ccggtactga ccacacgcgt gaacacattc cggtactggt
    14461 ttacggcccg aaagtaaaac cgggctcact gggtcaccgt gaaaccttcg cggatatcgg
    14521 ccagactctg gcaaaatatt ttggtacttc tgatatggaa tatggcaaag ccatgttctg
    14581 ataatgggtg cggcttgatg ccctcacccc ggccctctcc cacagggaga gggtgaaaac
    14641 atgtagataa ggataaaaca atggctaccc cacacattaa tgcagaaatg ggcgatttcg
    14701 ctgacgtagt tttgatgcca ggcgacccgc tgcgtgcaaa gtatattgct gaaactttcc
    14761 ttgaagatgc ccgtgaagtg aacaacgttc gcggtatgct gggcttcacc ggtacttaca
    14821 aaggccgcaa aatttccgta atgggtcacg gtatgggtat cccgtcctgc tccatctaca
    14881 ccaaagaact gatcaccgat ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg
    14941 cagttctgcc gcacgtaaaa ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt
    15001 ccaaagttaa ccgcatccgt tttaaagatc atgactttgc cgctatcgct gactttgaca
    15061 tggtgcgtaa cgcggtagac gcggctaaag cactgggcgt tgatgctcgc gtgggtaacc
    15121 tgttctccgc tgacctgttc tactctccgg acggcgaaat gttcgacgtg atggaaaaat
    15181 acggcatcct cggcgtggaa atggaagcgg ctggtatcta cggcgtggct gcagagtttg
    15241 gcgcgaaagc cctgaccatc tgcaccgtgt ctgaccacat ccgcactcac gagcagacca
    15301 ctgccgctga gcgtcagacc accttcaacg acatgatcaa aatcgcactg gaatccgttc
    15361 tgctgggcga taaagagtaa ttgtgtttcg ctgaaaggcg aacgtcgtgt taagccggag
    15421 caggagactg ctccggcttt ttagtatcta ttcatcaata
//
LOCUS       DAZFKM010000117        15336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFKM010000117 DAZFKM010000000
VERSION     DAZFKM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            118..1407
                     /gene="hpaG"
                     /locus_tag="ACHKAY_003793"
     CDS             118..1407
                     /gene="hpaG"
                     /locus_tag="ACHKAY_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO5702386.1"
                     /translation="MKGTIFVVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1404..2870
                     /gene="hpaE"
                     /locus_tag="ACHKAY_003794"
     CDS             1404..2870
                     /gene="hpaE"
                     /locus_tag="ACHKAY_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO5702387.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2872..3723
                     /gene="hpaD"
                     /locus_tag="ACHKAY_003795"
     CDS             2872..3723
                     /gene="hpaD"
                     /locus_tag="ACHKAY_003795"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO5702388.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3733..4113
                     /locus_tag="ACHKAY_003796"
     CDS             3733..4113
                     /locus_tag="ACHKAY_003796"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO5702389.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4224..5027
                     /gene="hpaH"
                     /locus_tag="ACHKAY_003797"
     CDS             4224..5027
                     /gene="hpaH"
                     /locus_tag="ACHKAY_003797"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO5702390.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5038..5826
                     /gene="hpaI"
                     /locus_tag="ACHKAY_003798"
     CDS             5038..5826
                     /gene="hpaI"
                     /locus_tag="ACHKAY_003798"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO5702391.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            6001..7377
                     /gene="hpaX"
                     /locus_tag="ACHKAY_003799"
     CDS             6001..7377
                     /gene="hpaX"
                     /locus_tag="ACHKAY_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO5702392.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7387..8277
                     /gene="hpaA"
                     /locus_tag="ACHKAY_003800"
     CDS             7387..8277
                     /gene="hpaA"
                     /locus_tag="ACHKAY_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO5702393.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8528..10090
                     /gene="hpaB"
                     /locus_tag="ACHKAY_003801"
     CDS             8528..10090
                     /gene="hpaB"
                     /locus_tag="ACHKAY_003801"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO5702394.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10108..10620
                     /locus_tag="ACHKAY_003802"
     CDS             10108..10620
                     /locus_tag="ACHKAY_003802"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO5702395.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            11013..13163
                     /gene="btsT"
                     /locus_tag="ACHKAY_003803"
     CDS             11013..13163
                     /gene="btsT"
                     /locus_tag="ACHKAY_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO5702396.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13294..13497
                     /locus_tag="ACHKAY_003804"
     CDS             13294..13497
                     /locus_tag="ACHKAY_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO5702397.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13508..14464
                     /gene="yjiA"
                     /locus_tag="ACHKAY_003805"
     CDS             13508..14464
                     /gene="yjiA"
                     /locus_tag="ACHKAY_003805"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO5702398.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14551..14742)
                     /locus_tag="ACHKAY_003806"
     CDS             complement(14551..14742)
                     /locus_tag="ACHKAY_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO5702399.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14782..>15336)
                     /locus_tag="ACHKAY_003807"
     CDS             complement(14782..>15336)
                     /locus_tag="ACHKAY_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702400.1"
                     /translation="LILIRSMIIACIPIIYLFASSCASSLFLSLSNLNITLTPWVEYF
                     WKGMAFMLLFFMLMQLVIYFAFLTLWAKLSVYRLFILAGAFIVSTILVAFASKNVEGI
                     SYYVLKSTIDFEWRNQVKCGELNISRPDERYFGFNTDKYTVFYSNREGKWGVNELKCK
                     KGSDRRDAYSIENVSEYNVPGWLK"
BASE COUNT         3627 a         4171 c         4134 g         3404 t
ORIGIN      
        1 aatagtttgt taattagatc acatttacat cacttacttt tgccaaacta cgcaatcggg
       61 aataaaagta atcattaaca tattaatgat taagatgatc gaatccgagg agtggtaatg
      121 aaaggcacta tcttcgtcgt agcgttgaac catcgcagcc aacttgatgc atggcaggaa
      181 gcgttccagc aatcccccta caaagccccg cctaaaactg cggtctggtt tattaaaccg
      241 cgcaatacgg tgattggttg cggtgaaccg attccctttc cacagggtga aaaggtactg
      301 agcggtgcga ctgttgcgct gattgtggga aaaacggcga cgaaagtacg tgaagaagat
      361 gcggcagagt acatcgccgg atatgcgctg gctaacgacg tcagcctgcc ggaagagagc
      421 ttttaccgcc cggcaatcaa agcaaaatgt cgtgatggat tctgccccat tggcgaaacc
      481 gtggctctca gcaatgtcga taatctgacc atctataccg agatcaacgg gcgtcctgcc
      541 gatcactgga ataccgccga tttacaacgt aacgccgcgc agttgctgag cgccctgagc
      601 gaatttgcca cactgaatcc aggcgatgcc attctgctcg gcacgccaca ggcgcgcgtg
      661 gaaatacagc caggcgatcg cgttcgtgtt ctcgcagaag gtttcccgcc gctggaaaat
      721 ccggtagtgg acgaacgtga agtgaccacg cgcaagagct tcccaacgca gccacacccg
      781 cacggcacgc tgtttgccct cggcctgaac tacgccgacc acgccagcga actggaattt
      841 aagccaccgg aagaaccgct ggtgttcctg aaagcgccaa ataccctcac tggcgataac
      901 cagacctccg tgcgtccaaa caatattgaa tacatgcact atgaagcgga gctggtggta
      961 gttattggca aacaggcgcg taacgtcagc gaagccgatg ccatggatta tgtcgcgggc
     1021 tacaccgtgt gtaacgacta cgccattcgc gactatctgg aaaactacta ccgccctaac
     1081 ctgcgggtaa aaagccgcga cggactgacg ccgatgcttt caaccatcgt gccgaaagag
     1141 gcgatcccgg acccgcataa tctgaccctt cgcaccttcg tcaacggcga gttacgccag
     1201 caaggcacca ccgccgatct gatcttcagc gtgcccttcc tgatcgccta cttaagcgaa
     1261 tttatgaccc tgaatccggg cgacatgatc gccaccggca caccaaaagg cttatctgac
     1321 gtggtgcctg gcgatgaagt agtggtggaa gtagaaggcg tgggctgcct ggtgaaccga
     1381 attgtgagtg aggaaacagc gaaatgaaaa aagtaaatca ttggatcaac ggcaaaaatg
     1441 ttgcaggtaa cgactacttc cagaccacca atccggcaac gggtgaagtg ctggcggatg
     1501 tggcctctgg cggtgaagcg gagatcaatc aggcggtagc ggcagcgaaa gaggcgttcc
     1561 cgaaatgggc caatctgccg atgaaagaac gtgcgcgcct gatgcgccgt ctgggcgatc
     1621 tgatcgacca gaacgtgcca gagatcgccg cgatggaaac cgcggacacc ggcctgccga
     1681 tccatcagac caaaaatgtg ttgatcccac gcgcttccca caactttgaa tttttcgcgg
     1741 aagtctgcca gcagatgaac ggcaagacct atccggttga cgacaagatg ctcaactaca
     1801 cgctggtgca gccggtgggc gtttgtgcgc tggtatcgcc gtggaacgta ccgtttatga
     1861 ccgccacatg gaaggtcgcg ccgtgtctgg cgctgggcaa tactgcggtg ctgaagatgt
     1921 cagaactctc cccgctgacc gctgaccgcc tgggtgagct ggcgctggaa gccggtattc
     1981 cggcgggcgt gctgaacgtg gtacagggct acggcgcaac cgcaggggat gcgctggtcc
     2041 gtcatcatga cgtgcgtgcc gtgtcgttca ccggcggtac cgccaccggg cgcaacatca
     2101 tgaaaaacgc cgggctgaaa aaatactcca tggaactggg cggaaaatcg ccagtgctga
     2161 tttttgaaga tgccgatatt gaacgcgcgc tggacgccgc cctgttcacc atcttctcga
     2221 tcaacggcga gcgctgcacc gccggttcgc gcatctttat tcagcaaagc atctacccgg
     2281 aattcgttaa acgctttgcc gaacgcgcca accgtctgcg cgtgggcgat ccgaacgatc
     2341 cgaataccca ggttggggca cttatcagcc agcaacactg ggaaaaagtc tccggctata
     2401 tccgtctcgg cattgaagaa ggcgcaaccc tgctggcggg cggcccggat aaaccgtctg
     2461 acctgcctgc gcacctgaaa ggcggcaact tcctgcgccc aacggtgctg gcagacgtag
     2521 ataaccgtat gcgcgtcgcc caggaagaga ttttcgggcc ggtcgcctgc ctgctgccgt
     2581 ttaaagacga agccgaaggc ttacgcctgg caaacgacgt ggagtacggc ctcgcgtcgt
     2641 acatctggac acaggatgtc agcaaagtgt tacgcctggc gcgtggcatt gaagcaggca
     2701 tggtgttcgt caatacccag aacgtccgcg acctgcgcca gccgtttggc ggcgtaaaag
     2761 cctccggcac cgggcgtgaa ggcggtgagt acagcttcga agtgttcgcg gaaatgaaga
     2821 acgtctgcat ttccatgggc gaccatccaa ttccgaaatg gggagtctga tatgggtaag
     2881 ttagcgttag ccgccaagat cacgcacgta ccgtcgatgt atctctctga actaccagga
     2941 aaaaaccacg gttgtcgcca gggtgcgatc gacgggcata aagagatcag caagcgttgc
     3001 cgggaaatgg gcgtcgatac cattatcgtt ttcgacaccc actggctggt caacagtgct
     3061 tatcacatca actgtgcaga ccattttgaa ggcgtctaca ccagtaacga actgccgcat
     3121 tttattcgtg acatgaccta caactacgag ggcaacccgg agttggggca gctaattgcc
     3181 gatgaagcct taaagctcgg cgtgcgggca aaagcgcaca atattcccag cctgaaactg
     3241 gaatacggca cgctggtgcc gatgcgctac atgaatgaag acaagcactt caaagtggtc
     3301 tccatttcgg ctttctgcac ggttcacgat tttgccgaca gccgcaagct gggcgaagcg
     3361 attctgaaag cgatcgaaca gtacgacggc accgtggcgg tccttgccag cggttcgtta
     3421 tcgcaccgct ttattgacga tcagcgtgcg gaagaaggga tgaacagcta cacccgcgag
     3481 ttcgaccgcc agatggacga gcgcgtggtg aagctgtggc gcgaaggcca gttcaaagag
     3541 ttttgcaata tgctgccgga gtacgccgac tactgctacg gcgaaggcaa tatgcacgac
     3601 acggtgatgc tgctggggat gctcggctgg gataaatacg acggcaaggt ggagtttatc
     3661 accgagctat tcccaagctc tggcaccggt caggttaacg ctgttttccc gcttcccgcg
     3721 taaggacgtt ttatgccgca ctttatcgtt gaatgcagtg ataacatccg cgaagaagcc
     3781 gacctgccgg ggttgttcgc caaagtgaat ccgacgctgg cagccacggg tatttttccg
     3841 ctggcgggta ttcgcagccg cgtgcattgg gtcgatacct ggcagatggc cgacgggcag
     3901 catgattatg ccttcgtgca tatgacgttg aaaatcggcg caggtcgcag cctggaaagc
     3961 cgccagcagg cgggtgaaat gctgtttgaa ctgattaaaa cgcacttcgc cgccctgatg
     4021 gagagccgcc tgctggcgtt gtcgtttgag attgaagagc tgcatccgac gctgaatttt
     4081 aaacaaaaca acgtgcacgc attgtttaag tagcgcgcag attgcccggt ggcgctgcgc
     4141 ttaccgggcc tacaaaaccc caaaccgtac accgtaggcc ggataaggcg cagccgcatc
     4201 cggcaatgcc acaggatatc gctatgttcg ataaacacac ccacaccctg atcgcccagc
     4261 gtctggatca ggcagaaaaa cagcgcgaac agatccgcgc gatctcgctg gattacccgg
     4321 agatcaccat cgaagacgct tacgcggtgc agcgtgaatg ggttcgcctg aaaatcgccg
     4381 aaggtcgcac gctgaaaggc cacaaaatcg gcctgacctc gaaagcgatg caggcaagct
     4441 cgcagatcag cgaaccggat tacggcgcgc tgctggatga catgttcttc cacgatggca
     4501 gcgatatccc gaccgatcgc tttatcgtgc cgcgcattga agtggagctg gctttcgtgc
     4561 tggcaaaacc gctgcgtggg ccaaactgca cgctgttcga cgtctacaac gccacggact
     4621 acgtgatccc ggcgctggag ctgatcgacg ctcgctgcca caacatcgat ccggaaaccc
     4681 agcgcccgcg caaagttttc gacaccattt ctgataacgc cgccaacgcc ggggtgatcc
     4741 tcggaggtcg tccgatcaaa ccagacgaac tggatctgcg ttggatctcc gccctgatgt
     4801 atcgcaatgg cgtgattgaa gaaaccggcg tcgccgctgg cgtgctgaat catccggcaa
     4861 acggcgtggc ctggctggcg aacaaactcg caccgtatga cgtacaactg gaagccgggc
     4921 aaatcattct cggtggttcg ttcacccgcc cggttccggc gcgtaagggc gacaccttcc
     4981 acgtcgatta cggcaacatg ggttccatta gctgccgctt tgtttaagga gagaacgatg
     5041 gaaaacagct ttaaagcggc gctgaaagca ggccgtccgc agatcggatt atggctgggg
     5101 ctgagtagca gctacagcgc agagttactg gccggagcag gattcgactg gttgttgatc
     5161 gacggtgagc acgcgccgaa taacgtgcaa accgtgctca ctcagctaca ggcgattgcg
     5221 ccctacccca gccagccggt agtacgtccg tcgtggaacg atccggtgca aatcaaacaa
     5281 ctgctggacg tcggcacaca aaccttgctg gtgccgatgg tacaaaacgc tgacgaagcc
     5341 cgtgaagcgg tacgcgccac ccgttatccc cccgccggta ttcgcggtgt gggcagtgcg
     5401 ctggcccgag cctcgcgctg gaatcgcatt cctgattacc tgcaaaaagc caacgatcaa
     5461 atgtgcgtgc tggtgcagat cgaaacgcgt gaggcaatga agaacttacc gcagattctg
     5521 gacgtggaag gggtcgacgg cgtgtttatc ggcccggcgg atctgagcgc cgatatgggt
     5581 tatgccggta atcctcagca cccggaagta caggccgcga ttgagcaggc gatcgtgcag
     5641 atccgcgaag cgggcaaagc gccggggatc ctgatcgcca atgagcaact ggcaaaacgc
     5701 tatctggaac tgggcgcgct gtttgtcgcc gtcggcgttg acaccaccct gctcgcccgc
     5761 gccgccgaag cgctggcagc acggtttggt gcgcaggcta cagcgattaa gcccgacgtg
     5821 tattaatgcc ggatgcgctg cgcttatccg gcctacactc gcaccaaacc gtaggccgga
     5881 taaggcgttt acgccgcatc cggcaaaaag ccgtaccaaa tcgcggaccg gataaggcgt
     5941 ttgtgcgcat ccgccataaa ccttgtcgta ccctacaaaa atcccattag aggaagtaaa
     6001 atgagcgaca cctcacctgc cataccggag agtatcgatc cggcgaatca gcataaagcg
     6061 ctgactgccg gacaacaggc ggttattaag aagctatttc gccgcctgat cgtctttctg
     6121 ttcgtgctgt ttatcttctc gttccttgat cgcatcaaca tcggctttgc cggactcacg
     6181 atgggacgcg acctcggtct gagcgccacc atgtttggcc tcgctaccac cttgttctac
     6241 gccgcttacg tcatcttcgg cattcccagc aacattatgc tgagtattgt cggtgcacgg
     6301 cgctggatcg ccaccatcat ggtgctttgg ggcatcgcct ctactgccac catgtttgcc
     6361 actggcccca ccagcttata cgtactgcgt atactggttg gcattaccga agccggcttt
     6421 ctgcctggca ttctgctgta tttaaccttc tggtttccgg cctatttccg cgcccgtgcc
     6481 aacgccttgt ttatggtagc aatgccggta acgacagcgt tgggatcgat cgtttccggc
     6541 tacattttgt cgctggatgg cgtaatggca ttaaaaggct ggcagtggct gtttttgctg
     6601 gaaggcttcc cgtcggtatt actcggcgtc atggtgtggt tctggcttga tgactcaccg
     6661 gacaaagcta agtggctgac gaaagaagac aaaaaatgcc tgcaagagat gatggataac
     6721 gatcgtctga cgctggttca gccagaggga gccatcagcc accacgccat gcaacaacgc
     6781 agtatgtggc gggagatctt cactccggtg gtgatgatgt ataccctggc gtatttctgc
     6841 ctgaccaaca cacttagtgc gatcagcatc tggacaccgc agatcctgca aagctttaat
     6901 cagggcagca gtaatatcac catcggcctg ctggccgccg taccgcagat ttgtaccatt
     6961 ctcgggatga tctactggag ccgtcactca gatcgccgcc aggaacgaag gcatcacacc
     7021 gcccttcctt atttgttcgc tgccgctggt tggttactgg cttcggcaac tgatcacaac
     7081 atgatccaga tgctggggat cattatggct tcgaccggat cattcagcgc aatggcgatt
     7141 ttctggacaa caccggatca gtccatcagc ctgcgggcac gagcgatcgg tattgcggtg
     7201 atcaacgcca ctggcaacat tggttcagcg ttaagtccgt ttatgatcgg ctggttgaaa
     7261 gatctgaccg gcagctttaa cagtggattg tggtttgttg ccacgctgct ggtgattggt
     7321 gcggggatta tctgggcgat tccaatgcag tcctcccgtc cgcgagcgac cccgtaagga
     7381 acgacgatgt gtgaccgtca gattgccaat attgatatca gcaaagagta cgatgaaagc
     7441 ctgggcacgg acgatgtgca ttatcagtcg ttcgcccgca tggcggcctt ttttggccgc
     7501 catatgctgc cacatcgcca cgaacagtac tttcagatgc atttcctcaa tagcggacag
     7561 attgagctac agcttgacga tcatcgctac tcggtggaag cgcccctgtt tgtcctgacg
     7621 ccgccgtcag tacctcatgc gtttattacg gagtctgatg ctgacggtca tgtattgacg
     7681 gtacgggaag atctgatctg gcccctgctg gaagttcttt atccgggcac tcgggaaacc
     7741 ttcggcctgc cggggatttg cctgtcactg gcagataaac ccgacgaact ggcggcgctg
     7801 gaacactatt ggcaactgat agagcgggaa tcggtagaac aactgcctgg tcgggaacac
     7861 accctgacgt tactggcaca ggcagtgttc accctactgc tgcgtaacgc aaaactcgac
     7921 gaccatgccg ccagcggaat gcgcggagaa ttaaaactgt tccagcgttt tcatatgctg
     7981 attgaaagcc attttcatca gcactggaca gtaccggatt acgctaacga attgcatatc
     8041 accgaatcac gcctcactga catctgccgc cgctttgcca accgtccgcc aaaacggttg
     8101 attttcgaca ggcagctgcg agaagccaag cggctgctgc tgttttctga taacgccgtg
     8161 aacaatattg cctggcaact cggttttaag gatccggctt attttgcgcg cttttttaat
     8221 cgcttagtcg gttgctcgcc cagtgcttat cgcgccaaaa aagtacctgt gacgtgaaaa
     8281 tcccttagcc ttaacgggaa accaggcacc acctgctatt cccctttctc cgcctggagc
     8341 aaggggattt agtaacgtta aaaatcgcta aaagcgacct cgatcacaaa accgtacccg
     8401 atctgaaaag taccagccat tgtcccaaaa gtgtcttccc gtaatcgccc ctgcaagctt
     8461 caattgaata acaaaaacaa cacacaaaat taacaatcgt attccgatta atactgtaga
     8521 ggtcgacatg aaaccagaag atttccgcgc cagtacccaa cgtccgttca ccggggaaga
     8581 gtatctgaaa agcctgcagg atggtcgcga gatctatatc tatggcgagc gagtgaaaga
     8641 cgtcaccact catccggcat ttcgtaatgc ggcagcgtct gttgcccaac tgtacgacgc
     8701 gctacacaaa ccggagatgc aggactctct gtgctggaac accgacaccg gtagcggcgg
     8761 ctatacccat aaattcttcc gcgtggcgaa aagtgccgac gacctgcgcc agcaacgcga
     8821 cgccatcgct gagtggtcac gcctgagcta tggctggatg ggccgtaccc cagactacaa
     8881 agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt tcgagcagaa
     8941 cgcccgtaac tggtataccc gtattcagga aactggcctc tactttaacc acgcgattgt
     9001 taacccaccg atcgatcgtc atttgccgac cgataaagtg aaagacgttt acatcaagct
     9061 ggaaaaagag actgacgccg ggattatcgt cagcggtgcg aaagtggttg ccaccaactc
     9121 ggcgctgact cactacaaca tgattggctt cggctcggca caagtgatgg gcgaaaaccc
     9181 ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat ggcgtgaaat taatctcccg
     9241 cgcctcttat gagatggtcg cgggtgctac cggctcgccg tacgactacc cgctctccag
     9301 ccgctttgat gagaatgatg cgattctggt gatggataac gtgctgatcc catgggaaaa
     9361 cgtactgatc taccgcgatt tcgatcgctg ccgtcgctgg acgatggaag gcggttttgc
     9421 ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg aaactagact tcattacggc
     9481 actgctgaaa aaatcactcg aatgtaccgg caccctggag ttccgtggtg tgcaggccga
     9541 tctcggtgaa gtggtggcgt ggcgcaacac cttctgggca ttgagtgact cgatgtgttc
     9601 agaagcgaca ccatgggtca acggtgctta tttaccggat catgccgcac tgcaaaccta
     9661 tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac attatcgaac gcaacgttac
     9721 cagtggcctg atctatctcc cttccagtgc ccgtgacctg aacaatccgc agatcgacca
     9781 gtatctggcg aagtatgtgc gcggttcgaa cggtatggat cacgtccagc gcatcaagat
     9841 cctcaaactg atgtgggacg ccattggcag cgagtttggt ggtcgtcacg aactgtatga
     9901 aatcaactac tccggtagcc aggatgagat tcgcctgcag tgtctgcgcc aggcacaaag
     9961 ctccggcaat atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg aatacgacca
    10021 gaacggctgg actgtgccgc acctgcacaa caacgacgat atcaacatgc tggataagct
    10081 gctgaaataa cgcagcagga ggttaagatg caattagatg aacaacgcct gcgctttcgt
    10141 gacgcaatgg ccagcctgtc ggcagcggta aatattatca ccaccgaggg cgacgccgga
    10201 caatgcggaa ttacggcaac ggccgtctgc tcgatcacgg atacaccacc gtcgctgatg
    10261 gtgtgcatta acgccaacag tgcgatgaac ccggtttttc agggcaacgg taagttgtgc
    10321 gtcaacgtcc tcaatcatga gcaggagctg atggcccgcc acttcgcggg catgacaggc
    10381 atggcgatgg aagagcgttt tagcctctca tgctggcaaa aaggtccgct ggcacagccg
    10441 gtgctgaaag gttcgctggc cagtcttgaa ggtgagatcc gcgatgtgca ggcaattggc
    10501 acacatctgg tgtatctggt ggagattaaa aacatcatcc tcagtgcaga aggtcacgga
    10561 cttatctact ttaaacgccg tttccatccg gtgatgctgg aaatggaagc tgcgatttaa
    10621 gctccagccc cttcacgcat tcgtcgaagg ggcattttta ttgtgatctc aatcacatat
    10681 ccacccgatg aataattctt acccaaccgc tcaccgccat tcctgaccgc ttacatccct
    10741 aaaataacca ctcagttatt taccttactt tacgcgtgcg taactctggc aacatcactg
    10801 caggatagcg gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg cttcggccta
    10861 tgattaacaa attactctat ggattttggt ttgcaggaag gcagcaagtg agtgaatccc
    10921 cgggagctta taacagtaag tgacaggggt gaacgaacgc agctgccgca cctgtaagcc
    10981 aaaagacgac gagtaaaatg ccaggtttta ctatggatac gaaaaaacta ttcaagcaca
    11041 taccctgggt gattctcgga atcatcggtg cattctgcct cgcggtagtt gcattacgtc
    11101 ggggggagca cgtcagcgcc ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg
    11161 cgtatcgcta ctacagtctg tatatcgccc agaaggtgat gaaactcgac cccacgcgcg
    11221 cgacgcctgc ggttattaac aacgacggtc tgaactacgt tccgaccaac cgttacgtgt
    11281 tgtttggtca ccacttcgcc gctatcgccg gtgctggtcc gctggttggt ccggttctcg
    11341 ccgcgcagat gggctacctg cccggcacgc tgtggctgct ggcgggggta gtgctggccg
    11401 gtgcggtaca ggactttatg gtgctgttta tctcctctcg ccgtaacggc gcatctctcg
    11461 gtgagatgat caaagaagag atgggaccag taccggggac tatcgcgctg tttggctgtt
    11521 tcttaatcat gatcatcatc cttgccgtcc tggcgctgat tgtggttaaa gccctggcag
    11581 aaagtccgtg gggtgtcttc accgtttgct caaccgtacc gattgcgctg tttatgggta
    11641 tctacatgcg ctttatccgt ccggggcgtg tgggtgaagt ctctgtaatc ggtatcgtgc
    11701 tgctggttgc ctctatctac ttcggtggcg tgattgctca cgatccgtac tggggtccgg
    11761 cactgacctt taaagacacc accattacct tcgcgctgat tggctatgcg tttgtttccg
    11821 cactgctgcc ggtgtggctg atcctcgcac cgcgcgacta tctggcaacc ttcctgaaaa
    11881 tcggcgttat cgtcggcctg gcgttgggta tcgtggtgct gaacccggaa ctgaaaatgc
    11941 ctgcgatgac ccagtacatt gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt
    12001 tcctgttcat caccatcgcc tgtggtgcgg tatctggctt ccacgcgctg atctcttccg
    12061 gtacgacgcc gaaactgctg gctaacgaaa ccgacgcgcg tttcattggc tacggcgcaa
    12121 tgctgatgga gtccttcgtg gcgattatgg cgctggttgc agcgtccatc atcgaaccgg
    12181 gtctttactt cgcgatgaac accccgcctg cgggccttgg catcaccatg cctaacctgc
    12241 atgaaatggg tggcgagaac gcgccgatca tcatggcgca gctgaaagac gttaccgcac
    12301 acgcggcggc gaccgtcagc tcctggggct tcgtgatttc gccagagcag atcctgcaaa
    12361 ccgcgaaaga cattggtgag ccttctgtcc tgaaccgtgc aggtggtgcg ccgacactgg
    12421 cggtaggtat cgcgcacgtg ttccacaaag tgctgccgat ggctgacatg ggcttctggt
    12481 atcacttcgg tattctgttc gaagccctgt tcatcctgac cgcgctggat gcgggtaccc
    12541 gttctggccg ctttatgctg caggacctgc tgggtaactt catcccgttc ctgaaaaaaa
    12601 ccgactctct ggttgctggt atcatcggta ctgcgggctg tgtgggtctg tggggctacc
    12661 tgctgtatca gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg
    12721 gtatctccaa ccagatgctg gcagccgtag cgctggtact gggcaccgtt gtgctgatta
    12781 agatgaagcg cacccaatac atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct
    12841 gcaccacctg ggcgctgggc ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct
    12901 tctacatggc aagccagtac aaagagaaga ttgctaacgg tactgacctg acggcacagc
    12961 agattgccaa catgaaccac atcgttgtga acaactacac caacgcgggc ctgagtattc
    13021 tgttcctgat tgtggtgtac agcatcatct tctacggttt caaaacctgg cttgcggtgc
    13081 gtaacagcga caaacgtact gacaaagaaa caccgtacgt tccaatcccg gaaggcggcg
    13141 tgaagatctc ttcgcaccac taaccggttg ccggatgcgt tgcgcgtatc cggcctacaa
    13201 agtgtctatc gtaggccgga taaagcgttt acgccgcatc cggcaaatcc ctagcccgga
    13261 ccttccgggc tttacgcata tctggaaggc aaaatgtttg gtaacttagg acaggcaaaa
    13321 aaatatctcg gccaggcggc gaagatgttg attggcattc cagactacga caactacgtc
    13381 gagcatatga agaccaacca tcccgacaag ccgtacatga gctatgaaga attcttccgc
    13441 gaacgccaga acgcgcgcta cggcggcgat ggtaaaggcg gtatgcgctg ttgttaatgg
    13501 agagagcatg aacccgattg cagttaccct actcaccggt tttttaggcg cgggcaaaac
    13561 caccctgctg cgccatattc ttaacgaaca acacggctac aagattgccg tgattgaaaa
    13621 cgaattcggc gaagtctccg ttgatgatca actgattggc gaccgcgcca cgcagatcaa
    13681 aacgctgacc aacggctgca tctgctgttc gcgctccaac gagctggaag acgcactact
    13741 cgacctgctg gataatctcg acaagggcaa tattcagttc gaccgtctgg tcattgaatg
    13801 caccggcatg gccgatcccg gtccgattat tcagaccttt ttctcccatg agattttatg
    13861 ccagcgttac ctgctggacg gcgtgattgc gctggtggat gcggtacatg ccgatgagca
    13921 gatgaaccag ttcaccatcg cccagtccca ggttggctac gccgaccgta ttctgctgac
    13981 caaaaccgac gtcgcaggcg aagcagaaaa actgcgtgaa cgcctggcgc gcatcaacgc
    14041 ccgtgcaccg gtctacaccg tcacccacgg cgacatcgac ctgggcctgc tattcaacac
    14101 caacggtttt atgctggaag agaacgtcgt cagcaccaaa ccgcgcttcc actttatcgc
    14161 ggataaacaa aacgatattt cgtcgattgt ggtggaactg gattacccgg tagatatcag
    14221 cgaagtttcc cgggtgatgg aaaacctgct gctggaatcg gcagataaac tgctgcgtta
    14281 caaagggatg ctgtggattg acggcgaacc taaccgcctg ctattccagg gcgtccagcg
    14341 cctctacagc gccgactggg accgcccgtg gggcgatgaa aaaccgcata gcacgatggt
    14401 gtttatcggg attcagctgc cggaggaaga gattcgggcg gcgtttgcgg ggttgaggaa
    14461 gtaatcgtga aaggttgagt gtaaagcagc tcaatgcccc atttagcagt gaggggggaa
    14521 acagaacgac gtgctcccct ccgctaagtt ttattgcgcg tgaatacctg gatctggcaa
    14581 aaaattgccg cgtttggcgt cagaccatgc ttcgctacca gaagcaggaa tgtcgctgta
    14641 atcaatgtct tcgtcggatt tattaaccaa agccatgagt tcagcttctt gctgcacatc
    14701 aagtgtgtag agtgtgcccc gtttatgttt aaccatgctc atttatgacc agaaccttta
    14761 cgatttaatt ataaagatga cttatttcag ccatccaggc acgttatatt ctgagacatt
    14821 ctcaatggaa tatgcatctc tcctgtcaga acctttctta cacttcaact cattgacccc
    14881 ccatttaccc tctctattgg aataaaatac agtatattta tcggtattaa acccgaaata
    14941 tctttcatcc ggacgtgaaa tattcaactc tccacattta acctgattac gccattcaaa
    15001 atctatggtg ctttttaaaa cgtagtacga aataccttct acattttttg aagcaaaagc
    15061 cactaaaata gtagatacga taaatgctcc ggcaagtata aacaacctat atacacttaa
    15121 tttagcccac agagttaaaa aagcaaaata gataacaagt tgcattaaca taaagaaaag
    15181 caacataaaa gccatccctt tccagaaata ctcaacccat ggcgttagtg tgatattgag
    15241 attactcaaa cttaaaaaca aactggaagc acaactactt gcaaacaaat atataattgg
    15301 tatgcaagct ataatcatgg acctaataag tattag
//
LOCUS       DAZFKM010000118        15251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFKM010000118 DAZFKM010000000
VERSION     DAZFKM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(223..1047)
                     /gene="iclR"
                     /locus_tag="ACHKAY_003808"
     CDS             complement(223..1047)
                     /gene="iclR"
                     /locus_tag="ACHKAY_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO5702401.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1247..4930
                     /gene="metH"
                     /locus_tag="ACHKAY_003809"
     CDS             1247..4930
                     /gene="metH"
                     /locus_tag="ACHKAY_003809"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO5702402.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTTSISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5150..6781
                     /locus_tag="ACHKAY_003810"
     CDS             5150..6781
                     /locus_tag="ACHKAY_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO5702403.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6872..7561)
                     /gene="pepE"
                     /locus_tag="ACHKAY_003811"
     CDS             complement(6872..7561)
                     /gene="pepE"
                     /locus_tag="ACHKAY_003811"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO5702404.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7944..9173)
                     /gene="sorE"
                     /locus_tag="ACHKAY_003812"
     CDS             complement(7944..9173)
                     /gene="sorE"
                     /locus_tag="ACHKAY_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO5702405.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQLGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9225..10047)
                     /locus_tag="ACHKAY_003813"
                     /pseudo
     CDS             complement(9225..10047)
                     /locus_tag="ACHKAY_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(10058..10855)
                     /locus_tag="ACHKAY_003814"
     CDS             complement(10058..10855)
                     /locus_tag="ACHKAY_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO5702406.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10921..11415)
                     /locus_tag="ACHKAY_003815"
     CDS             complement(10921..11415)
                     /locus_tag="ACHKAY_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO5702407.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11415..11822)
                     /locus_tag="ACHKAY_003816"
     CDS             complement(11415..11822)
                     /locus_tag="ACHKAY_003816"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO5702408.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11832..12638)
                     /locus_tag="ACHKAY_003817"
     CDS             complement(11832..12638)
                     /locus_tag="ACHKAY_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO5702409.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12708..13655)
                     /locus_tag="ACHKAY_003818"
     CDS             complement(12708..13655)
                     /locus_tag="ACHKAY_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO5702410.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            14003..14875
                     /gene="rluF"
                     /locus_tag="ACHKAY_003819"
     CDS             14003..14875
                     /gene="rluF"
                     /locus_tag="ACHKAY_003819"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO5702411.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14876..15148)
                     /locus_tag="ACHKAY_003820"
     CDS             complement(14876..15148)
                     /locus_tag="ACHKAY_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO5702412.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3613 a         3996 c         4011 g         3631 t
ORIGIN      
        1 gctcaatctc ataatgcagc cgtaaaagtt tcggtggaat gagatcttgc gattttgtta
       61 atgattaagt agtttgatgt ttaaaaataa tgctgagtta ataaaagtgt gtgaagtgta
      121 tgacattaag ttattttggc gtcaatgcga ttaacagaca cccttattct attgccactc
      181 aggtatgatg ggcagaatat tgcctctgcc cgccagaaaa agtcagcgca ttccaccgta
      241 cgccagcgtc acttccttcg ccgctttaat caccatcgcg ccaaactcgg tcacgcggtc
      301 atcggtaata cgtgaaatcg gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc
      361 atcgaaaata cacgctgcaa ggcaacgtag ccccagtgca tgttcctcat cgtcaaatga
      421 ataaccccgt ttgcgcgttt gggcgagatc ttcttttaaa tgcacaggag acaccagcgt
      481 tgcgtgggta taggcatgta accctttgcg gtgcagcagc ttcgtcacct gttcttcgct
      541 cagttgggct aaaaaggctt tacccgcacc ggaagcgtgc atcggcaatt taccgccgat
      601 aggcgcggac attcgcatca gatgcgtaca ctgtacctgg tcgataataa tcgcttcgtg
      661 atcgctttga tcaagcaccg ccatattgac cgtttcgcca gactcttcca ttaaattgcg
      721 caggataggg tgaacaatcg ctaacaaatt acggctctgg agaaagctgc tgccgaccat
      781 aaaggcatgt gcgccgattg cccaatgtcc cagttcgccg acctgacgca cgaaaccctg
      841 ctgttgcatc gtggttagca ggcggtgggt cgtggaattg ggtaacccgg cttgttgcgc
      901 cagctctgtg agtgccacac tgccattgga ttcggcaatc cactccagta atttcaggcc
      961 acgcgttaaa gactgaacct gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc
     1021 gcgtttcgcg ggaatgggtg cgaccatgac agcctccttt ttctgtatcg tggaaatcat
     1081 tttcattttt attgttagct aatgcaatag ttgctgaact gatccgatga gttaatgttg
     1141 aacaaatctc atgttgcgtg gtggtcgctt ttaccacaga tgcgtttatg ccagtatggt
     1201 ttgttgaatt tttattaaat ctgggttgag cgtgtcggga gcaagtgtga gcagcaaagt
     1261 ggaacaactg cgtgcgcagt taaatgaacg tattctggtg ctggacggcg gtatgggcac
     1321 catgatccag agctatcgac tgaacgaagc cgattttcgt ggtgaacgct ttgccgactg
     1381 gccatgcgat ctcaaaggca acaacgacct gctggtgctc agcaaaccgg aagtgatcgc
     1441 cgctatccac aacgcctact ttgaagcggg cgcggatatc atcgaaacca acaccttcaa
     1501 ctccacgacc attgcgatgg cggattacca gatggaatcc ctgtcggcgg aaatcaactt
     1561 tgcggcggcg aaactggcgc gcgcctgtgc tgacgagtgg accgcgcgca cgccggagaa
     1621 accgcgctac gttgccggtg ttctcggccc aaccaaccgc acgacgtcta tttctccgga
     1681 cgtcaacgat ccggcattcc gcaacatcac ttttgaccag ctggtggcgg cctatcgcga
     1741 atccaccaaa gcgctggtgg aaggtggtgc ggatctgatc ctgattgaaa ccgttttcga
     1801 cacccttaac gccaaagcgg cggtttttgc ggtgaaaacg gagtttgaag cgctgggcgt
     1861 tgagctgccg attatgatct ccggcaccat caccgacgcc tccgggcgca cgctctccgg
     1921 gcagaccacc gaagcatttt acaactcatt gcgccacgcc gaagctctga cctttggcct
     1981 gaactgtgcg ctggggcccg atgaactgcg ccagtacgtg caggagctgt cacggattgc
     2041 ggaatgctac gtcaccgcgc acccgaacgc cgggctaccc aacgcctttg gtgagtacga
     2101 tctcgacgcc gacacgatgg caaaacagat acgtgaatgg gcgcaagcgg gttttctcaa
     2161 tatcgtcggc ggctgctgtg gcaccacgcc acaacatatt gcagcgatga gtcgtgcagt
     2221 agaaggatta gcgccgcgca aactgccgga aatccccgta gcctgccgtt tgtccggcct
     2281 ggagccgctg aacattggcg aagatagcct gtttgtgaac gtgggtgaac gcaccaacgt
     2341 caccggttcc gctaagttca agcgcctgat caaagaagag aaatacagcg aggcactgga
     2401 tgtggcccgc cagcaggtgg aaaacggtgc gcagattatc gatatcaaca tggatgaagg
     2461 gatgctcgac gccgaagcgg cgatggtgcg ttttctcaat ctgattgccg gtgaaccgga
     2521 tatcgctcgc gtgccgatta tgatcgactc ctcaaaatgg gatgtcattg aaaaaggtct
     2581 gaagtgtatc cagggcaaag gcattgttaa ctctatctcg atgaaagagg gcgtcgacgc
     2641 ctttatccat cacgcgaagc tcttgcgtcg ttacggtgcg gcagtggtgg taatggcctt
     2701 tgacgaacag ggacaggccg atacccgcgc acggaaaatc gagatttgcc gtcgggcgta
     2761 caaaatcctc accgaagagg ttggcttccc gccagaagat atcatcttcg acccgaacat
     2821 cttcgcggtt gccaccggca ttgaagagca caacaactac gcgcaggact ttatcggcgc
     2881 gtgtgaagac atcaaacgcg aactgccgca cgcgctaatt tccggcggcg tatctaacgt
     2941 ttctttctcg ttccgtggca acgatccggt gcgcgaagcc attcacgcag tgttcctcta
     3001 ctacgctatt cgcaatggca tggatatggg gatcgtcaat gccgggcagc tggcgattta
     3061 cgacgacctg cccgctgaac tgcgcgacgc ggtggaagat gtgattctta atcgtcgcga
     3121 tgatggcact gagcgtttac tggagcttgc cgagaaatat cgcggcagca aaaccgacga
     3181 caccgctaac gcccagcagg cggagtggcg ctcgtgggaa gtgaataaac gtctggaata
     3241 ctcgctggtc aaaggcatta ccgagtttat cgagcaggat accgaagaag cccgccagca
     3301 ggctacgcgc ccgattgaag tgattgaagg cccgttgatg gacggcatga acgtggtcgg
     3361 cgatctgttt ggcgaaggga aaatgttcct gccacaggtg gtcaaatcgg cgcgcgtcat
     3421 gaaacaggcg gtggcgtatc tcgaaccgtt tattgaagcc agcaaagagc agggcaaaac
     3481 caacggcaag atggtgatcg ccaccgtgaa gggcgacgtc cacgacatcg gtaaaaatat
     3541 cgttggtgtg gtgctgcaat gtaacaacta cgaaattgtc gatctcggcg ttatggtgcc
     3601 tgcggaaaaa attctccgta ccgctaaaga agtgaatgct gatctgattg gactttcggg
     3661 gcttatcacg ccgtcgctgg acgagatggt taacgtggcg aaagagatgg agcgtcaggg
     3721 cttcaccatc ccgctattga ttggaggcgc gacgacctca aaagcgcaca cggcggtgaa
     3781 aattgagcag aactacagcg gcccgacggt gtatgtacag aacgcatcgc gcaccgttgg
     3841 tgtggtggcg gcgctgcttt ccgatactca gcgtgatgat tttgtcgctc gtacccgcaa
     3901 ggagtacgaa accgtacgta ttcagcacgg gcgtaagaaa ccgcgcacac caccggtcac
     3961 gctggaagcg gcgcgcgata acgatttcgc ttttgactgg caggcttaca cgccgccggt
     4021 ggcgcaccgt ctcggcgtgc aggaagttga agccagcatc gaaactctgc gtaattacat
     4081 cgactggaca ccgttcttta tgacctggtc gctggccggg aagtatccgc gcattctgga
     4141 agatgaagtg gtgggcgttg aggcgcagcg gctgtttaaa gacgccaacg acatgctgga
     4201 taaattaagc gccgagaaaa cgctgaatcc gcgtggcgtg gtgggcctgt tcccggcaaa
     4261 ccgtgtgggc gatgacattg aaatctaccg tgacgaaacg cgcacccatg tgatcaacgt
     4321 cagccaccat ctgcgtcaac agaccgaaaa aacgggattc gctaactact gtctcgctga
     4381 cttcgttgcg ccgaagcttt ctggtaaagc agattacatc ggcgcatttg ccgtgactgg
     4441 cgggctggaa gaggacgcac tggctgatgc ctttgaagcg cagcacgatg attacaacaa
     4501 aatcatggtg aaagcgcttg ccgaccgttt agccgaagcc tttgcggagt atctccatga
     4561 gcgtgtgcgt aaagtctact ggggctatgc gccgaacgag aacctcagca acgaagaact
     4621 gatccgcgaa aactaccagg gcatccgtcc ggcaccgggc tacccggcct gcccggaaca
     4681 tacggaaaaa gccaccatct gggagctgct ggaagtggaa aaacataccg gcatgaaact
     4741 cacagaatct ttcgccatgt ggcccggtgc atcggtttcg ggttggtatt tcagccaccc
     4801 ggacagcaag tactacgcgg tggcgcaaat tcagcgcgat caggttgaag actatgcccg
     4861 ccgtaaaggt atgagcgtct ccgatgttga gcgctggctg gcaccgaatc tggggtatga
     4921 cgcggactga ttcacaaatc tgtcactttt ccttacaaca aacagggcgc tcaatgagtg
     4981 ccctgtctct ttattaatat gaaacactta tactggaaac aggctggaat aatcttagcc
     5041 ggaaaaacga atgtcaaaag gcacagtaaa gatgccagtc agcgtgtttc cggtcctttg
     5101 gtcattcaat tgcttcagga acatataacg ataaggagaa cctgaactcg tgttaacgct
     5161 gcttcacctg ctttctgccg tcgccctgct ggtctggggg actcatattg ttcgaaccgg
     5221 cgtaatgcgc gtcttcggcg cgcgtttgcg tactgtcctt agccggagcg tcgaaaaaaa
     5281 gccgctcgcc ttttgcgcgg ggatcggcgt taccgcactg gtgcagagca gtaatgccac
     5341 caccatgctg gtgacctcgt ttgtcgctca ggatctggta gccctcgcac cggctctggt
     5401 cattgtgctg ggggcagatg tcgggacggc gctaatggcg cgtattctca ccttcgactt
     5461 atcctggctg tcaccgttac ttatttttat cggcgtgatt tttttcctcg gacgcaaaca
     5521 gtcacgcgcc gggcaactgg gccgcgtcgg tattggtctt gggctgattt tactggcgct
     5581 ggagttgatt gtgcaggccg taacgccgat cacccaggca aacggcgttc aggtgatctt
     5641 tgcctcgctg accggcgata ttctgctgga tgcgctgatt ggcgcgatgt tcgccattat
     5701 cagctactcc agccttgctg ctgtactgct gactgcgact ctgaccgccg caggcattat
     5761 ctccttcccc gtggcgctct gtctggtgat tggtgctaac ctcggttccg gcctgctggc
     5821 gatgctcaac aacagtgccg ccaatgccgc agcccgccgt gtcgcgctgg gtagtctgct
     5881 gtttaagctg gtgggtagcc tgattatcct gccgtttgtc catttgctgg cagagacaat
     5941 ggggaagctg tcattgccaa aagcggaact ggtgatctat ttccacgtct tctacaacct
     6001 tgtacgttgc ctggtcatgc tgccatttgt tgacccgatg gcacggtttt gcaaaacgat
     6061 tattcgcgat gaaccggaac tggataccca gctacggcct aaacatctgg atgtcagcgc
     6121 gctggatacg cccacgcttg ctctggcgaa cgccgcgcgc gaaaccctgc gcattggcga
     6181 cgccatggaa cagatgatgg aagggctgaa taaagtgatg cacggcgagc cacggcagga
     6241 gaaagagctg cgtaagctgg cagatgatat caacgttctc tataccgcca ttaagctgta
     6301 tctggcgcgg atgccaaaag aggaactggc agaagaagag tcgcgtcgct gggcggagat
     6361 tattgaaatg tcgctcaacc ttgaacaggc ttccgatatc gtcgagcgca tgggcagcga
     6421 aattgccgat aaatcgctgg cagcacggcg ggcattttcg cttgatgggt tgaaggaact
     6481 ggatgcgctc tatgagcaat tgctcagtaa tttaaagctg gcaatgtcgg tgttcttctc
     6541 tggcgatgtc accagcgctc gtcgtttgcg tcgcagcaaa catcgttttc gcattcttaa
     6601 tcgccgctat tcccacgccc acgtcgatcg cctgcatcag caaaacgtgc agagcattga
     6661 aaccagttcg ctacatttag gcttactggg agatatgcag cgcctgaact cgctgttttg
     6721 ttcggtggct tacagtgtgc tggaacagcc ggatgaagat gaaggacggg acgagtatta
     6781 atattgtgaa tcctgtaggc ctgataagac gcgtcaagcg tcgcatcagg cactatgaac
     6841 aagatgccgg atcatgcatc cggcaattta cttaaaaacg gtgaccagct tctagcggca
     6901 ccgcttcttc accagcctta aacacataag tggtgttcgg gccacccagc gtagcgtgac
     6961 ctttactcac tgtgatccag ttaccttccg gtagaccaat aatcgtcagt tctggcgcga
     7021 cgaccagcag ttcgcgaata cgctgctcac gggtttcacc tttatggcct tccggcagcg
     7081 cgttggtgaa gtgcgggttg atttgcagcg ggaacagatt tagcgcatcg aaaccttgcg
     7141 gatcgacaat cggcatatcg ttggtggtac gaatggttgg gcaagcaagg ttagcgcctg
     7201 cgctccagcc aatatacagc gcaccacgtt taaccacgtc agtaattggt gccagcagcc
     7261 cgcgctcgcg gcactgtttc agcaactgga aagtattccc gcccccgaca atcacgatct
     7321 cagcattttc aatcgcggca acgggatcga caacgctatg aataccggtg acagatacac
     7381 ccagcggagc gagaaccgca gccgttttcg ctgtgtaatc atcccaggtc tgcgttacgc
     7441 cagcgaaagg aataaatacc gctgaacggc gaccctgcaa ctgttcagca atcagcggca
     7501 gcgcatgttc cagccaggct ttacccggca gcgtcgagtt actcaataaa agcagttcca
     7561 ttacttctcc agttagtaag aaatcacggg aggcatgcta ccactgcgaa tttaaccact
     7621 tactgacatg gctcacgatg tcacgtattg caccgggcgc tgagactgat gacaaacaca
     7681 gcattgcctg atgcgctacg cttaccaggc ctaccagata actgcaatat attgaatttt
     7741 cgagattatg taggccgaat aaggctttat tccgcatccg gcataaacaa tgtgtacttt
     7801 gccaccaatt tgagcgcccc ttaaggcgct gatgtttttt tatccttctc gtgcacattg
     7861 ggccgcttca gcaatcagcc acacatcagg tcgcgaagtt gccgcggcga ccagccagcg
     7921 acatgcagag ttaagtagcg cgattaatca tgggaaattg cctctgcatg agtgagcaga
     7981 tattgctccg cctcaccgga ccagatcccc tgatgacgcg ccagaatcgc cgccagtgct
     8041 tgatcctgaa tctgcgtgag tgacgtcagc ggcaggtatt tcccggtata caccagcttt
     8101 ttgccgccgc cgacggcagg caattcaagc gtggtttcgc ccgcggcatt cagcccaaga
     8161 atatgtgtta ccacttttgc ggcctgcact tttttctctt caatcagttt gaccgccgcg
     8221 cgcatgtcgt cggtattgcc gcctgacgtg cccacgtagt gggtaaatgc ataatgcaca
     8281 tcgtagaaat taattggcgc gctgaaatgt ttatcctgcg gtccggcgaa gaaattcagg
     8341 caaccatctg tcgccagcaa ggaagaggcg agagtcacca gtccttcatt aggcacaaag
     8401 acgaaaatat catcgaagcc gtgaccgcca ctcagcgcca ttagcgtatc aaatgctgca
     8461 tcggcggcat tgagataatg aatcagtgtt tgcggttctg atggatagtg cttgcgcgca
     8521 taactcaatt tatcgttgtc ggtatcggta atgacgagca gcgacgggtt aacgggtcca
     8581 tgtagcgcat aatcaatcgc caacagtccc attggtccgg tgccgccgag gatcagcatg
     8641 cgcccttgcg ggcgaatccc catcgtgtgg ttataactac cttcctgaag atgatagttg
     8701 gcgttgaacg cgccaatcac gcaggaaagc ggttcaacca gtgagccttc aaaataggtt
     8761 tcgccgtcgt atgccagcag gcaatcttgt tccatgacct cgttgggaat aaccacatgc
     8821 gtggcttcgc cgcccaccca cgggaaggag tagccggggc agtccgggcg gtcggggagt
     8881 tgcaggttgg cctgaatcac ataacgctga cccagctgga atttgtgctg ccacttttta
     8941 cccacggcca gaatatcgcc gcaaaactcg tggccgatga tgatggggtt ggtcgccaca
     9001 tcgtcgggta cttttttatg gttttcaccc agattggcct ctttccagga agagaggcac
     9061 aggctgtcag tgaccaccgt cgcgaggatt tcatcctcct gcatttcagg aaggtcaaag
     9121 gtttccaggc gtaaatcacg tttaccataa agacgcagag ctgttgtttt cataaaccac
     9181 ctcatgttga gcgatggccc cggcagtcgt gccggggcga agaatcagga caggaagccc
     9241 agcgcattac cgataatccc taagccaaac agggcgaaaa tcagccatac cgggttaatt
     9301 tttttgttga gcagacgaac cataagcagc gtcagaccga gcgccagcaa accagggcaa
     9361 agttggtcga gaatgttctg cacggtcatg gtgacggtgg agccatcggc ggcatgcgtt
     9421 tgtgaaacca ccaacggtac gttgattgac gtccatttgg tgaccagcac gcccatcaca
     9481 aacagtccga gaatcgacgc gccttcggtg agtttttgca gcacattccc gcccatatcg
     9541 ctgacgatat tcaccccttt gcgaaagccg agctgtaggc cataccactt catcgccaga
     9601 cgcaccgcat tgaaaataaa gaaaaaaaag cgggccgagg atgttgccag aaagtgccag
     9661 agatgcgccg agcgcagcgg taatcgggcg cagcgttccc caaaccagtg gatcgccaac
     9721 ccctgccaat ggtcccatca gaccgacttt gatgccgtta atggtaccgt catcaatttc
     9781 cgcgccgttg gcccgcgcct cttccatggc ggcagtgacg ccgatgaccg ggccacatac
     9841 ggctggcgtg gtattgaaga acaccaggtg tcgcctgagc gccgcaacct gatcctcttt
     9901 taatgggtaa agtcgcttga tggcggggat catgtcgtag caaaagccca gcccgtgaat
     9961 acgttcaaag ttaaaggacg cctgttgcag gttggagcgc agaaacatgc tcaccagatc
    10021 gctgcgtgta atttttctct gttccatgat ggctccgtta atcgtcaagt tggtcaaggg
    10081 cggtggaggt ggtggcctgg ggttgaggtt cagctttacg ccactgtgga tttagctgga
    10141 tgtagaccag ggccatgatc acgccgacgc caccgaacgc cagcagactg agatcgaggt
    10201 agccacctgc gaggaaaccg aggaaaaaga aaggcatcaa atatttcacg cccatcatac
    10261 gtagcaccat ggcgtaaccg accaccacga taaaaccgcc agcaatttgc agaccgcgcg
    10321 tcacgaattc cggtatcgcg ctcagcatat tgctcaccat atcggcgctg acgaacagtg
    10381 agacaatcag tgccggaata gcaacgcgca gcgcctgcac gccaagcgcg gagacatgca
    10441 gaatatcgag agtgcgaaac cgcgcttctt ccgctgcttt atccgccgcg tgctggaaca
    10501 ctacggtgat ggtacgggca aacacagtca gcacctggcc tgccgcagcc acaggcaacg
    10561 cgatggcgat tccggtggcg atgctttgct gaccaacgat aaccagaatg gcggagatta
    10621 tgctggcgag tgcagaatcc ggagattgcg ccgcgccgac gttcatccag ccgagagcta
    10681 tcaattccag cgtaccaccg agtattattc cggtttttaa atcaccgaga attaaaccaa
    10741 tcaccgtaca ggcaattaac gggcgatgag tctgaaattc atccagcacg ctgcccattc
    10801 cggcaataca ggaaaaaaga aatatagcaa tgatttgtag ggtacttatt tccatgattt
    10861 ttcacctttt aagtgtcgtc atccctgcct gttaaagcat atgtacacag gcgctattat
    10921 ttaatttgcg attaactgtt cgtttatttt gtcgataata ttgattgagg gatctgacgc
    10981 tacgacgcgt aaatccagta tgacgccaag attatttaac tcatgaaatg cgttaatatc
    11041 atcatcatct aatgaaaccg ctttggttaa ctgtttttta ccgggccgcc aggccatgcc
    11101 gccaatattc agggtgtcta tttttacccc ctggcgaacc atcgctaagg catcctgtgg
    11161 gcgagtgaaa agataaaaaa cggtttcgtc ctgatattgc gggttgtgat aaacagcgac
    11221 ggctttttca atattgacca cgttaacctt cattcccggt ggcgcagcct gacgcaataa
    11281 tgtccggcga acttcatcgt tataaacttc gtcattgcag ataataatgc gctgggcgtt
    11341 agccactttc gaccagacgg tagtgacctg accgtggatc aggcggtcat caatgcgggc
    11401 gagcgtaatg ttcatcagaa atcctcctct gccgttgcca gttgtttcca gacaacgcag
    11461 gtttgctttg cgatttgcgt caggtgttca cacagttcat caacattcat gctgtcctgg
    11521 ttatcaacca gttcgagcgc cagcggcaga gaaaggccgc tgattacccg cagacgagga
    11581 ttgcgcatcg caagcatcgc tgccgcattc cacgggctgc cgcactgtaa atccacggca
    11641 atcagccact catcttgatt gtgaatgctt actaactttt ccagctttgc tacgatgtct
    11701 ccggcgttct cgccgggcac aaattcaacg gcttcaacat tggcgttgcc gtaaaccatc
    11761 tgaaccgatt ccagcatggc gcaagctaat ttgccgtggg cacaaaaaat ggcgttgacc
    11821 ataaagcctc cttagccgcg cgttttgccg cccgcaatgt tagtggttac tccggtgatg
    11881 tagctggcgc gttcagacaa cagataacaa acaaaatcag cgacttctgc taatcttccg
    11941 gcgcgcccaa taggaatggc gtttttggta tagccttcac gcagctgctc gacggtgata
    12001 ttgcgcgtcc acgccagcgc ctcttcatat tccggcgtgc gtagcccggt tttttccaga
    12061 atcccgggcg cgataccgac cacacggata ccgtgtttac ccagctcttt cgaccaggag
    12121 cgcgtgaagc tattaagcgc ggctttggta gcggcgtaac agctttggcc ttctgagcct
    12181 tccagtccac tttctgagga aacattcaca atcacgccat catgttgttt gaccatctgt
    12241 cgcgccaccg cctgcgacat cagaaaaacg cctttctggt tgatattgac cattttttcg
    12301 aatgcagctt cgttgagttc atactgcccg gcaggcgctt tctcatcgac cagcagacgc
    12361 gggaaattga ccccggcgtt attgaccaga ccgtcgatgc gaccaaaacg ctggataatt
    12421 tccgctaccg tatgatttac ctctttggcg ctggaaatat cggtcggcca aaactgatag
    12481 cctttatgac cttcatattg accatcgcca ccgtgaatat ctaccatctg tacgtttgcg
    12541 ccttgtgcta ataattcctc tacaatcgcc agaccaatac cggatgcgcc gccggtgacg
    12601 ataatgattt tatcctgcaa atttaaccac gtttgcataa cagactcctg attttagata
    12661 atagggaccc gccgtaattt ttattacggt aaacatcggg aagattttta tttcagtaat
    12721 agttcagctg tgctgctatt cgttaccaga caattaatat attttccacg cagtgcacca
    12781 ataattccgc tgtatttttc ttcactcatg gcaatgccaa tggaatatcg cgcctgcttt
    12841 aatttattca tttcgataga gagcgttttt tcgctcatat tcgtttcaac cattgcgccg
    12901 tgaatatcaa aaaagcgcga gcaaatatcg ccagcaacct ggcgggcatt caggtcgtca
    12961 ctctcttcac caccataaaa cgcatgccag ttagcgccgt cgcgaatggc cggtgagcca
    13021 attcccacca gggcgacatc cagattatcc cagtaggcag agatggtttt aaagtgctga
    13081 gactgcatga tcccattacg aattaatggg ttatccagca gagccggaaa atccgcgaga
    13141 tgcgattccc ctttcagctt cgctgccgcg ctgtaggtta atgtgtttac atgatagcgg
    13201 ctttcgagtt taccggacgg tccgccaata attggtacgc agattaactg ccgcgattgc
    13261 cccgcctgcg gcaagttttc aaccagtgcg ctcaccgcgc gaccccagga aaaaccgaca
    13321 atatcgccag gctccagcaa gcgatccagc agttgcgcgc cgtgtaaccc catcatcgcc
    13381 agttgagtct cttcatcctc atcatttccc gacaccacca caacgtcttt caggccaaac
    13441 ttttgcttca gttgctgctc cagccagaga ttttcgttgt agtcatagtt gatggcgatg
    13501 gtgacaattc cctgatcgcg gcctcgttta agcaagcggc tgatggtggt gcggtaaata
    13561 cgcagttcgc gcgcgatttg cgcctgcgtc atatcctgtt cgtaatagag ttgggcaatc
    13621 ttcacaatca aacggatatc gtcactgttt tccattgccg cctcctggtt ctgcacattt
    13681 gtgcaaatac gctttctcac cgcttgatct gatataaccc catcatgagg gcctttcaaa
    13741 gcgatttttc actctttgca tggggagaaa tagcgctttc tcttgcacat ttgtttctct
    13801 gcgtagtagt catcagcaca ggtgtgcagc gattcgtaat atgttgatta tccttatttt
    13861 gccaggcaaa tttgcatcgt acttccggag acacttttgt gataaaaggc gtggcagatc
    13921 acatttaatt tcgacgttgc cgcataaccc tgtttttccc cgctaagagg tacggtatat
    13981 tccgcgtttt acaggagaat ttatgctgcc cgactcatca gtccgtttaa ataaatacat
    14041 cagcgaaagc ggaatttgct cacgccgcga agcggatcgc tatatcgagc aaggcaatgt
    14101 gttccttaat ggcaagcgag ccaccattgg cgatcaggtg aaacccggcg acattgtgaa
    14161 agtaaacggt cagttgattg agcctcggga agccgaagat ttggtactta tcgccctgaa
    14221 caagcccgtc ggtattgtca gcaccaccga agatggcgag cgcgataaca ttgtcgattt
    14281 cgttaaccac agcaaacgcg tgttcccgat tggccgcctg gataaagact cccaggggct
    14341 gattttcctc accaatcacg gcgatctggt gaataagatc ctccgtgcgg gtaacgatca
    14401 tgagaaagag tatctggtga cggtcgataa accgattacc gatgaattta ttcgtggtat
    14461 gggggcgggg gtgccgatcc tcgggacggt gacgaaaaag tgcaaagtca aaaaagaagc
    14521 gccgtttgtc ttccgcatta ccctggtgca ggggctgaac cgtcagatcc ggcgcatgtg
    14581 cgagcatttc ggctatgaag tgaaaaagct ggaacgtacg cgcattatga acgtcagttt
    14641 aagcggcatt ccgctgggtg agtggcgcga tttaactgac gatgagttaa tcgacctgtt
    14701 taaactcatt gaaaattcct cttccgaggt aaaacctaaa gcgaaggcta aaccgaaaac
    14761 agcgggcatc aaacgtccag tcgttaagat ggaaaaaacg gcggaaaaag gcggtcgccc
    14821 ggcgtccaac ggtaagcgtt ttacctcgcc ggggcgtaaa aagaagggac gctaatcagc
    14881 gcctgccgcg cgtcgacgta tttgctgacc agctaaatga cgcttcagga tccggtttat
    14941 aagcctgctt tttcttcaac tggcgggctt ttttcgcttc agcctcacgc tcgaccatta
    15001 atttatcgat gtactctttc ttaatgctgt tggtttcgga gttggtcagt acgcgaccat
    15061 gcgcaatccg cgcccgatcc agcagtgttt taagttcacg ctgctcgcgt tcagtcatct
    15121 ctttttgggt aatacggggg agtgccatga tggtgccctc agtgagccag agagttcagt
    15181 gtacgggaat tcgtttgcga gctgaactga taatattcct ttgttctctt cttaaatatt
    15241 tatctttctt g
//
LOCUS       DAZFKM010000119        15178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFKM010000119 DAZFKM010000000
VERSION     DAZFKM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            178..513
                     /locus_tag="ACHKAY_003821"
     CDS             178..513
                     /locus_tag="ACHKAY_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO5702413.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            673..1116
                     /gene="yjdN"
                     /locus_tag="ACHKAY_003822"
     CDS             673..1116
                     /gene="yjdN"
                     /locus_tag="ACHKAY_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO5702414.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1249..2037
                     /gene="phnC"
                     /locus_tag="ACHKAY_003823"
     CDS             1249..2037
                     /gene="phnC"
                     /locus_tag="ACHKAY_003823"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO5702415.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2062..3078
                     /gene="phnD"
                     /locus_tag="ACHKAY_003824"
     CDS             2062..3078
                     /gene="phnD"
                     /locus_tag="ACHKAY_003824"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5702416.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3184..3963
                     /gene="phnE"
                     /locus_tag="ACHKAY_003825"
     CDS             3184..3963
                     /gene="phnE"
                     /locus_tag="ACHKAY_003825"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO5702417.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3984..4709
                     /gene="phnF"
                     /locus_tag="ACHKAY_003826"
     CDS             3984..4709
                     /gene="phnF"
                     /locus_tag="ACHKAY_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO5702418.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4710..5162
                     /gene="phnG"
                     /locus_tag="ACHKAY_003827"
     CDS             4710..5162
                     /gene="phnG"
                     /locus_tag="ACHKAY_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO5702419.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5159..5743
                     /gene="phnH"
                     /locus_tag="ACHKAY_003828"
     CDS             5159..5743
                     /gene="phnH"
                     /locus_tag="ACHKAY_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO5702420.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5743..6807
                     /gene="phnI"
                     /locus_tag="ACHKAY_003829"
     CDS             5743..6807
                     /gene="phnI"
                     /locus_tag="ACHKAY_003829"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO5702421.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6800..7645
                     /gene="phnJ"
                     /locus_tag="ACHKAY_003830"
     CDS             6800..7645
                     /gene="phnJ"
                     /locus_tag="ACHKAY_003830"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO5702422.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7642..8400
                     /gene="phnK"
                     /locus_tag="ACHKAY_003831"
     CDS             7642..8400
                     /gene="phnK"
                     /locus_tag="ACHKAY_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO5702423.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8511..9191
                     /gene="phnL"
                     /locus_tag="ACHKAY_003832"
     CDS             8511..9191
                     /gene="phnL"
                     /locus_tag="ACHKAY_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO5702424.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9188..10324
                     /gene="phnM"
                     /locus_tag="ACHKAY_003833"
     CDS             9188..10324
                     /gene="phnM"
                     /locus_tag="ACHKAY_003833"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO5702425.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10324..10881
                     /gene="phnN"
                     /locus_tag="ACHKAY_003834"
     CDS             10324..10881
                     /gene="phnN"
                     /locus_tag="ACHKAY_003834"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO5702426.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10868..11302
                     /gene="phnO"
                     /locus_tag="ACHKAY_003835"
     CDS             10868..11302
                     /gene="phnO"
                     /locus_tag="ACHKAY_003835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO5702427.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11304..12062
                     /gene="phnP"
                     /locus_tag="ACHKAY_003836"
     CDS             11304..12062
                     /gene="phnP"
                     /locus_tag="ACHKAY_003836"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO5702428.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12209..12538)
                     /gene="yjdP"
                     /locus_tag="ACHKAY_003837"
     CDS             complement(12209..12538)
                     /gene="yjdP"
                     /locus_tag="ACHKAY_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO5702429.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12607..12924)
                     /locus_tag="ACHKAY_003838"
     CDS             complement(12607..12924)
                     /locus_tag="ACHKAY_003838"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO5702430.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13177..15162
                     /gene="yjcS"
                     /locus_tag="ACHKAY_003839"
     CDS             13177..15162
                     /gene="yjcS"
                     /locus_tag="ACHKAY_003839"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO5702431.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3433 a         4495 c         4181 g         3069 t
ORIGIN      
        1 ataaatggag gcaaaaagat tattttctgt ttgcgtttcc tttcaaatga aaacgatcgt
       61 cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat tgcggccttt
      121 ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga aatccacatg
      181 tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa cggcatgtac
      241 atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga aagcgacgag
      301 ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt taccatcatt
      361 aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa agtgaaaaac
      421 atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt tggtccgatg
      481 aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta agccggataa
      541 ggcgctcgcg ccgcatccgg caacgatgcc gactgcctga tgcgacgctg accgcgtctt
      601 atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct tctcttaact
      661 gaggtcacca tcatgtcgtt aagtccctac ctctcttttg ccggtaactg tgccgacgca
      721 attgcttatt atcaacgtac gttgggcgct gaactgctct ataaaatcag cttcggcgaa
      781 atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca atttcccgat
      841 accgccatcg cccacgccaa cgtgcgcatt gccggaagcg acatcatgat gagcgatgct
      901 ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc gcaacaggtc
      961 gaagaaggaa aacgctggtt tgacaatctt gccgctaacg gaaaaatcga aatggcctgg
     1021 caggaaactt tctgggcgca tggctttggc aaagtcaccg ataaatttgg cgtaccgtgg
     1081 atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc cgccctcccg
     1141 cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt aaccaaatcg
     1201 tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat gcaaacgatt
     1261 atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aggcgctgca tgcggttgat
     1321 ctgaacattc atcacggtga aatggtggct ctgcttgggc cgtcgggttc cggcaaatcc
     1381 acccttttac gtcacttaag cggtttgatt accggcgata aatccgccgg cagccatatc
     1441 gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat ccgcaaaagc
     1501 cgcgccaaca ccggctacat cttccaacaa ttcaacctgg tgaaccgcct gagcgtactg
     1561 gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg ttttagctgg
     1621 ttcaccggcg agcagaaaca gcgcgcgtta caggcgctga cccgcgttgg catggtgcat
     1681 tttgcccatc agcgcgtttc caccctctcc ggcggacagc agcagcgtgt ggcgattgcc
     1741 cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc ctcgctggac
     1801 ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa cgacggcatc
     1861 accgtggtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga acgcatcgtc
     1921 gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga taacgaacgt
     1981 tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc tgcctgacat
     2041 ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc cttcaccagc
     2101 atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga aaaggcgctg
     2161 aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg gacgccattc
     2221 ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc cccagactac
     2281 gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta cggcaacctg
     2341 tcggcaatgg aagcggtgga tcgcgccaac ggccaggtct tcgcccagac ggtcgcggcg
     2401 gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc gatcaacaac
     2461 ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga tcctaactcc
     2521 acctctggct tcctcgtccc cggttactac gtctttgcca aaaacaatat ctccgccagc
     2581 gactttaagc gcaccgtcaa cgctgggcat gaaaccaacg cgctggccgt cgccaacaag
     2641 caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac ctccgcgcca
     2701 gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg cgatccgatc
     2761 gtctggcgta aaaacctttc cgaaaccacc aaagacaaga tctacgactt ctttatgaac
     2821 tacggcaaaa cgccggaaga aaaagcggtg ctggaacgcc tgggctgggc gccgttccgc
     2881 gcttccagcg acctgcaact ggtgccgatt cgccagctcg cgctgtttaa agagatgcag
     2941 ggcgtgaaaa gcaataaagg actgaatgag caggacaagc tggcaaaaac caccgagatt
     3001 caggcgcagc tggatgacct ggaccgcctg aacaacgcgt taagcgcgat gagttcggtg
     3061 agtaaagcgg tgcagtaaat cgtaggtcgg ataagacgcc ccggcgtcgc atccgacaat
     3121 gtgcaggcgt tgatgccgga tgcggtgcaa gcaccttatc cggcctacag accggagcca
     3181 aacatgcaaa ccatcaccat cgccccaccc aagcgcagct ggttctcgct tctgagctgg
     3241 gccgttgttc tcgccgtgtt ggtcgtctcg tggcagggcg cggaaatggc cccgctcacg
     3301 ctgattaaag acggcggcaa catggcgacg ttcgccgccg acttcttccc gcccgatttc
     3361 agccagtggc aggattacct caccgaaatg gccgtcacgc tgcaaatcgc cgtctggggc
     3421 acggcgctgg cggtggttct ctccatcccc ttcggcctga tgagcgccga aaacctggtg
     3481 ccgtggtggg tttaccagcc cgttcgccgc ctgatggacg cctgccgcgt cattaacgaa
     3541 atggtcttcg ccatgctgtt cgtggtcgcc gtcggcctcg gcccgttcgc tggcgtgctg
     3601 gcgctgttta tccacaccac cggcgtgctc tccaaactgc tttccgaagc ggtggaagcg
     3661 attgaacccg gcccggtgga aggcattcgc gccaccggcg ccaacaagct cgaagagatc
     3721 ctctacggcg tgctgccgca ggtgatgccg ctgctgatct cctactccct ctatcgcttc
     3781 gaatccaacg tccgctcggc gaccgtcgtc ggcatggtcg gtgcaggcgg gatcggcgtc
     3841 accctgtggg aagcgattcg cggtttccag ttccaacaaa cctgcgccct gatggtgctt
     3901 atcatcgtca cggtcagcct gctggatttc ctctctcaac ggttgcgtaa gcactttatc
     3961 tgataagcga ggcattgata tctatgcact tgtctacaca tccgaccagc tacccaacac
     4021 gctatcagga gatagccgca aaacttgagc aggagcttcg tcaacactac cgctgcggcg
     4081 actatcttcc cgccgagcag caactggcgg cgcgctttga ggtgaaccgc cacaccctgc
     4141 gccgcgccat cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag ggcgtcggcg
     4201 tgctggtgct gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt tttagccaga
     4261 acctgctgga ccagggtagc catcccacca gcgaaaaact gctgtcggta ttgcgccccg
     4321 cgtccggtca cgtcgctgac gcgctgggga ttaccgaggg cgagaacgtt atccatttgc
     4381 gcaccctgcg tcgcgtcaac ggcgtcgcgc tctgtttaat cgaccactac tttgcggacc
     4441 tcaccctttg gccgacgctg caacgcttcg acagcggttc gctgcacgat tttctgcgcg
     4501 agcaaaccgg aattgcgctg cgccgcagcc agacgcggat cagcgcccgt cgcgcccagg
     4561 ccaaagagtg ccagcgtctt gaaattccga atatgtcgcc gctgctgtgc gtgcgcaccc
     4621 ttaaccaccg tgacggtgaa agcagcccgg cggagtactc cgtcagcctg acgcgcgccg
     4681 acatgattga attcactatg gagcactgaa tgcacgcaga taccgcgacc cgccagcact
     4741 ggatgtccgt actggcgcac agccaaccgg ctgaactggc agcccgcctg aaagcactga
     4801 acatcaccgc cgactatgag gttatccgcg ccgccgaaac cggcctggtg cagattcagg
     4861 cgcggatggg cggcaccggc gaacgctttt ttgccggcga cgccacgctc acccgcgccg
     4921 ccgtacgcct gactgacggc acgctcggct acagctgggt gctggggcgc gacaaacagc
     4981 acgccgaacg ctgcgcgctg attgacgcgc tgatgcagca atctcgctat tttcaaaact
     5041 tatcagaaac ccttattgcc ccgctggacg ctgaccgtat ggcacgcatt gccgcacgcc
     5101 aggccgaagt gaacgccagc cgggtcgact tctttacgat ggttcgcgga gacaacgcat
     5161 gaccctggaa accgctttta tgcttcccgt gcaggatgcc cagcacagtt ttcgtcgcct
     5221 gttaaaggcc atgagcgagc cgggcgtgat tgtcgccctg catcagctca agcgcggctg
     5281 gcagccgctg aatatcgcca ccaccagcgt gctgctgacg ctggccgata acgacacgcc
     5341 ggtgtggctt gccgcgccat tgagtaacga tatcgtcagc cagagcctgc gttttcatac
     5401 caacgcgccg ctggtcagcc agccggaaca ggcgaccttc gcggtgacgg atgaggcgat
     5461 ttccagcgaa cagcttaacg ccctttccac cggcaccgcc gttgcgccgg aagcgggtgc
     5521 aacgctgatt ttacaggtcg ccagcctgag cggcgggcgc atgttacgtc tcaccggcgc
     5581 gggcattgcc gaagaacgaa tgatcgctcc gcagctgccg gagtgcattc tgcacgaact
     5641 caccgagcgc ccgcacccgt tcccgctcgg catcgacctg atcctgacct gcggcgagcg
     5701 cctgctggct attccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt tgccgtgaaa
     5761 gggggcgaga aggcgatcga cgccgctcac gccctgcaag agagccgacg ccggggcgat
     5821 accgatttgc ctgaactgag cgtcgcccag attgaacagc agcttaatct tgcggtggat
     5881 cgcgtgatga ccgaaggcgg cattgccgac cgcgaactgg cggcgctggc gctgaaacag
     5941 gccagcggcg ataacgtcga agcaattttc ctgctgcgcg cctaccgcac cacgctggcg
     6001 aagctcgcgg taagcgagcc gctcgacacc acagagatgc gtctcgaacg gcgtatttcg
     6061 gcggtttata aagacattcc cggcggccag ctgcttggcc caacctacga ctacacccat
     6121 cgcctgctcg atttcaccct gctggcaaac ggtgaagcgc cgacgctgac caccgccgac
     6181 agcgaacaac agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg gctggtgaag
     6241 tttgaagaag atagcggcgc acagccggat gacatcaccc gcacgccgcc ggtttacccc
     6301 tgctcacgct cctcccgcct gcaacaatta atgcgcgggg acgaaggcta tttgctggcg
     6361 ctggcctact ccacccagcg tggttacggg cgcaatcacc cgttcgcggg cgagatccgc
     6421 agtggttaca tcgacgtgtc gattgtgccg gaagagctgg gatttgcggt aaacgtcggc
     6481 gaactactga tgaccgagtg tgaaatggtc aacggtttta tcgacccgcc ggatgagccg
     6541 ccgcacttca cgcgcggcta cgggctggta ttcggcatga gcgagcgcaa agcgatggca
     6601 atggcgctgg tcgatcgtgc gttgcaggct ccggaatacg gcgagcacgc gacaggcccg
     6661 gcgcaggatg aagagtttgt gctggcacat gccgacaacg tcgaagccgc aggctttgtc
     6721 tcgcacctca aactccccca ctacgtcgat ttccaggccg aactggagct actcaaacgt
     6781 ctgcaacagg agaagaacca tggctaatct gagcggctac aactttgcct acctcgacga
     6841 gcagaccaaa cgcatgatcc gccgcgctat cttaaaagcg gtggcgatcc ccggttatca
     6901 ggtgccgttt ggcgggcgcg agatgccgat gccatacggc tggggaaccg gcggcataca
     6961 gctcaccgcc agcgtgattg gcgaaagcga cgtgctgaag gtgattgacc agggtgcgga
     7021 tgacaccacc aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg gggtaaacac
     7081 cactgaacgt acggacgatg cgacgcttat ccagacgcgt caccgcatcc ccgaaacgcc
     7141 gctgaccgaa gatcagatca ttatcttcca ggtgccaatc ccggaaccgc tgcgctttat
     7201 cgagccgcgc gaaacggaaa cccgcaccat gcacgcgctg gaagagtacg gcgtgatgca
     7261 ggtgaaactg tatgaagata tcgcccgctt cggtcatatc gccactacct atgcctatcc
     7321 ggtgaaggtg aacgggcgct acgtaatgga cccgtcgccg atcccgaaat tcgataaccc
     7381 aaaaatggac atgatgcccg ccctgcaact gttcggcgcg gggcgcgaga agcgcatcta
     7441 tgcggtgccg ccgtttaccc gcgtggaaag tctcgatttc gacgatcacc cgttcaccgt
     7501 tcagcagtgg gatgagccat gcgccatctg cggatcgacc cacagctatc ttgatgaagt
     7561 ggtgctggat gacgccggaa accgcatgtt tgtctgctcc gataccgatt attgccgcca
     7621 acagagcgag gcaaaaaacc aatgaatcaa ccgttacttt cggtcaataa cctgacccac
     7681 ctttacgcgc cgggcaaagg ctttagcgat gtctcttttg atttatggcc gggggaagtg
     7741 ctgggcattg tcggggagtc cggctccggg aagaccacgc tgctgaaatc aatctccgcg
     7801 cgcctgacgc cgcagcaggg ggagattcac tatgagagcc gttcgctgta cgcaatgagc
     7861 gaggccgacc gccgtcgcct gctgcgcacc gaatggggcg tagtgcatca gcatccactc
     7921 gacggcctgc gccgtcaggt ctcggcaggc ggcaatatcg gcgagcggct gatggcgacc
     7981 ggggcacgtc attacggcga tattcgtgcc accgcgcaga agtggctgga agaggtggaa
     8041 attcccgcca accggatcga cgacctgccg accacctttt ccggcggcat gcagcaacgt
     8101 ttgcagatcg cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga cgagccgacc
     8161 ggcggactgg atgtgtcggt acaggcacgc ctgctcgacc tgctgcgcgg cctggtggtg
     8221 gagctgaacc tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg cctgctggcg
     8281 gaccgtttgc tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac cgaccgcgtg
     8341 ctcgacgacc cgcaccatcc gtatacacaa ctgctggtgt catcggtttt gcagaattga
     8401 gccggtgccg gatgcggcgt aaacgcctta tccggcctac aaatgcgctc cccgtaggtc
     8461 ggataagacg cgtcagcgtc gcatccgaca cccgaaccac gaggcgaaaa atgattaacg
     8521 tacaaaacgt cagtaaaacc ttcatcctgc accagcaaaa cggcgtgcgc ctgcccgtct
     8581 tacaacgtgc ctcgctcacc gtcaacgcgg gcgaatgcgt ggtgctccac ggccattccg
     8641 gcagcggcaa atcaaccttg ctgcgctcgc tgtacgccaa ctatctgccc gacgaagggc
     8701 aaatccagat caaacacggt gacgaatggg tcgatctggt caccgcgcca gcgcgcaaag
     8761 tggtggagat ccgcaaaacc accgtcggct gggtgagcca gtttctgcgc gtcatcccgc
     8821 gtatttcagc actggaagtg gtgatgcagc cgctgctcga taccggcgtt ccgcgtgaag
     8881 cctgcgccgc taaagccgcg cgtctgctca cccgcctgaa cgtgccggaa cgcctgtggc
     8941 acctggcgcc atcaaccttt tccggtggcg aacagcagcg cgtcaacatc gcccgcggct
     9001 ttatcgtcga ctaccccatt ctgctgctcg acgaacctac cgcctcgctg gacgccaaaa
     9061 acagcgccgc cgtagtcgag ctgattcgcg aagccaaaac ccgtggcgcg gccatcgtcg
     9121 gcatcttcca tgacgaagcc gtacgtaatg acgtcgccga ccgcttgcac ccaatgggag
     9181 cctctgcatg attatcaata acgtcaaact ggtgctggaa aacgaagtgg tacacggttc
     9241 gctggaggtg caggatggcg aaatccgcgc ctttgccgaa agccagagcc gcctgccaga
     9301 agcgatggac ggcgaaggtg gctggctact acccggcctg attgagctgc ataccgacaa
     9361 cctcgataaa ttcttcaccc cgcgcccgaa agtcgactgg cccgcccatt cggcgatgag
     9421 cagccacgac gcgctgatgg tggcaagcgg catcaccacc gtactggacg ccgtggcgat
     9481 tggcgacgtg cgcgacggcg gcgatcggct ggagaatctg gagaagatga tcaacgccat
     9541 cgaagagacg caaaaacgcg gcgtcaaccg cgccgagcac cgcctgcacc tgcgctgcga
     9601 actgccgcat cacaccacac tgccgctgtt tgaaaaactg gttcagcgcg aaccggtgac
     9661 gctggtgtcg ctgatggacc actcaccggg ccagcgccag ttcgctaacc gcgagaagta
     9721 tcgcgaatat tatcagggca aatactccct caccgatgcg cagatgcagc agtacgaaga
     9781 agagcaattg gcgctcgccg cacgctggtc gcagccgaat cgcgaatcca tcgccgccct
     9841 gtgccacgcg cgacaaattg cgctcgccag ccacgatgac gccacgcacg cccacgttgc
     9901 cgaatcccac caacttggca gcgtgatcgc cgaatttccc accacgttcg aagcggcgga
     9961 agcctcgcgt aagcatggca tgaacgtgct gatgggtgcg ccaaatatcg tgcgtggcgg
    10021 ctcgcactcc ggcaatgtgg cggccagtga actggcgcag cttggtctgc tggatatcct
    10081 ctcttccgac tactaccccg ccagcctgct ggatgcggcg ttccgcgtcg ccgatgacga
    10141 gagcaaccgc tttacgctac cgcaggcggt gaggctggtg accaaaaatc cggcgcaggc
    10201 gctgaatctt caggatcgcg gggtgattgg cgagggtaaa cgcgctgacc tggtgctggc
    10261 gcatcgccag ggcaatcaca ttcatatcga ccacgtctgg cgtcagggta aaagggtgtt
    10321 ctgatgatgg gaaaactgat ttggttaatg gggccgtccg gctccgggaa agacagcctg
    10381 ctggcggaac tccgcctgcg ggaacaaacg cagttactgg tggcgcatcg ctacatcacg
    10441 cgcgacgcca gcgccggaag tgaaaaccat atcgccctga gtgagcggga gttttttacc
    10501 cgcgcggggc aaaacctgct ggccttaagc tggcacgcta acggtctgta ttatggcgtc
    10561 ggcatcgaga ttgacctctg gctgcacgct ggattcgacg tagtcgtcaa cggttcacgc
    10621 gcgcatttgc cgcaggcacg ggcgcgctat caatcggcgc tgctgcccgt ctgtttacag
    10681 gtttcgccgg agatcctgcg ccagcgcctg gaaaaccgtg gccgtgaaaa cgccagtgaa
    10741 attaacgccc ggctggcgcg cgccgcccgc tatactccac aggattgcca tacgctcaac
    10801 aatgacggca gcctgcgcca gtcggtcgac acactgctga cgctgattca tcagaaggag
    10861 aaacaccatg cctgcttgtg agcttcgccc ggccacgcag tacgataccg acgcggttta
    10921 cgcgctgatt tgtgagctaa aacaggcgga gtttgaccac cacgcgtttc gcgtgggctt
    10981 taacgccaac ctgcgcgacc cgaacatgcg ctaccatctg gcgctgctgg atggcgaagt
    11041 tgtcggcatg atcggcctgc atttgcagtt tcatctgcat catgtcaact ggatcggcga
    11101 aattcaggaa ctggtggtga tgccgcaggc gcgcggcctg aacgtcggta gcaagttgct
    11161 ggcgtgggca gaagaagaag cccgccaggc cggggccgaa atgaccgagc tttcgaccaa
    11221 cgtgaagcgc cacgacgcgc accgtttcta tctgcgagaa ggctacgagc agagccactt
    11281 ccgcttcacc aaggcgctgt aacatgagcc tgaccctcac gctcaccggc actggcggcg
    11341 cacagggcgt tccggcatgg ggctgcgagt gcgcggcctg cgccagagcg cggcgctcgc
    11401 cgcagtatcg ccgccagcca tgcagcggcg tagtgaagtt taacgacgcg atcaccctga
    11461 tcgacgccgg gcggcacgat ctcaccgatc gctggtcgcc tggatcgttc cagcagtttt
    11521 tgctgacgca ctaccatatg gatcacgtcc aggggctatt tccgctacgc tggggcgtgg
    11581 gcgatgtgat cccggtctac ggcccgccgg atgaacaggg ctgcgacgat ctgtttaaac
    11641 atccgggcct gcttgatttc agccacacag tggaaccgtt tgtggtgttt gatttgcagg
    11701 ggttgcaggt cacgccgctg ccgcttaacc actcaaaact gaccttcggt tatctgctgg
    11761 aaacggcaca tagccgggtg gcgtggctgt ctgacaccgc tggcctgccg gaaaaaacgc
    11821 tgaaattttt actcaataat catccgcagg tgatggtgat tgattgcagt cacccgccgc
    11881 gcgcggatgc accgcgtaat cactgtgatt taaataccgt gcttgcgctg aatcaggtta
    11941 tccgctcgcc acaggtgatt ctgacccata tcagccacca gtttgatgcg tggctgatgg
    12001 aaaacgcact accgtcaggg tttgaggtgg ggtttgatgg gatggagatt ggggtggcgt
    12061 gatgagaggg aatgtgcgcg ctggccccct caccctaacc ctctccccag aggggcgagg
    12121 ggaccgattg tgctcaatat tgaatattgc gctcgttttc tccctctccc cattggggtg
    12181 aggggcgatg cctgctccat acccaacctc atcgcccata ctcatcttcc attctccgct
    12241 cttcatcctc cagttgccga cgctcctgat caagctggcg ctggcgatcg tccagctgcc
    12301 tgcggcgatc ttcaaactgg cggcggcggt cgtcatattg tctgcgccga tcgtcgctca
    12361 cttcacgctg ccagccgtcg tcgcgcgaat cttcatagtc tcgcccacgg tcagggttat
    12421 aagcgtcatt aatcgcctgc tgaatattac caatagtatc gtcgataata tcagcctggg
    12481 ccggaacggt ggacagcgtg agcagggtga ataaaagaaa tagcggaaag cgtttcatta
    12541 gccaacctca aaaagaaact ctatccacat tagtcattac tcatccatgc aagtagtgga
    12601 tgaatctcaa tttctccgct gctctattgc cgtaatcgcc tccacgcgtt gttgatgacg
    12661 accgccttcg tactgtgcgc ccagccacgc atccacaatc atttttgcca gttcgaggcc
    12721 aaccactcgt gaaccaaaag ccagtgcaac ttcactggca taatgcggat aatcagtgcg
    12781 ctctgacgac ctggtgtctt tatcaatcac ctcactcatt gaccatgaat aaaaggctgg
    12841 cacagcaaca cagaatcctg cggtgccagc gacatgaccg cccattgctc cggtacgttt
    12901 tttaaaaaga aacaccggtt gcattgataa attgtgctca gtatttgccg aagtacaata
    12961 tatataataa atatataaaa cacctcatat tatttacaga gataataaat tcgcgtaatt
    13021 ttcccccttc agcacaaatg cctaataaaa tggatcgttc cgctcacact tccacttatt
    13081 aaagtcgcaa atacccaatt atgataacgc cagctttaaa tgacgtattt tgcatggatt
    13141 acatatttac gacacaaatg ataaatggag atttatatga ataactctcg gttattccgt
    13201 ttgagcagga ttgttattgc gttaactgct gccagcggca tgatggtaaa taccgctaac
    13261 gcgaaagagg aagcgaaagc cgccactcaa tatacccaac aggttaatca gaattacgcc
    13321 aaatcattac cgtttagcga tcgtcaggat tttgacgacg ctcagcgtgg atttatcgct
    13381 ccgctgctgg atgaaggtat tctgcgcgat gctaatggca aaccatatta tcgcggggaa
    13441 gattataaat ttgatatcaa tgcgcccgcg cccgaaaccg ttaaccctag cctgtggcgt
    13501 cagtcgcagt taaacggtat ttccggcctg tttaaagtca ccgacagaat gtaccaggtt
    13561 cgcggtcagg atatctccaa catcaccttt atcgaaggtg ataccgggat tattgtcatc
    13621 gacccgctgg tgacgccacc gagcgcaaaa gccgcccttg acctttactt ccaaaatcgc
    13681 ccgcaaaaac cgattgttgc ggttatttat acccacagcc ataccgacca ctatggcggc
    13741 gtgaaaggca ttatctctga agctgatgtg aaatccggta aagtgcaggt tatcgcccct
    13801 gctggcttta tggacgaagc catcagcgaa aacgtactgg cgggtaatat tatgagccgc
    13861 cgtgcacttt actcctacgg cctgctgctg gcgcataacc ctcagggtaa catcggcaat
    13921 ggtcttggcg taacgctggc atcgggctac ccgagcatca tcgcaccgaa caaaaccatc
    13981 accaaaaccg gtgagaagat gattatcgac ggcctagagt ttgacttcct gatgacccca
    14041 ggtagcgaag caccagccga aatgcacttc tatattccgg ccctgaaagc gctgtgtacc
    14101 gccgagaacg ccacgcatac cctgcacaac ttctacactc tacgcggcgc gaaaacccgc
    14161 gacaccagca agtggaccga gtatctgaac gaaacgctgg atatgtgggg taacgacgcg
    14221 gaagtcctgt ttatgccgca cacctggccg gtctggggca ataagcatat caatgattat
    14281 attggtaaat atcgcgatac tatcaagtac attcacgacc agaccctgca cctggcgaac
    14341 cagggctaca ccatgaatga aatcggcgac atgattaaac tgccgcctgc acttgccaat
    14401 aactgggcca gccgtggcta ttacggttct gtcagccaca acgcccgcgc ggtgtataac
    14461 ttctatcttg gctattacga cggtaacccg gctaacctgc atccgtatgg tcaggtggag
    14521 atgggtaaac gttacgtgca ggcgctgggc ggttctgccc gtgtcatcaa cctggcgcaa
    14581 gaagcgaaca agcaaggtga ttaccgctgg tcggcagaac tgctgaaaca ggtgattgcc
    14641 gccaacccgg gtgaccaggt cgcgaagaat ctgcaagcga ataactttga acagctgggc
    14701 tatcaggccg agtccgccac ctggcgcggt ttctacctga ccggcgcgaa agagctgcgc
    14761 gaaggggtgc ataagttcag ccacggcacc accggttccc cggacaccat tcgcgggatg
    14821 tcggtcgaaa tgctgttcga ctttatgtcc gttcgcctcg atagcgcgaa agccgcgggt
    14881 aaaaatatca gcctgaactt caatatgagc aatggcgata acctcaacct gacgctgaac
    14941 gatagcgtgc ttaactaccg taaaacactg caatcccaag ctgacgcctc tttctacatc
    15001 agccgtgaag atctgcacgc cgtgctgacc gggcaagcca aaatggcgga tctggtaaaa
    15061 gcgaagaaag ccaaaattat tggcaatggc gcgaaactgg aagaaattat cgcctgtctg
    15121 gataatttcg atttgtgggt gaatatcgta accccaaatt aatgtcactc gcttttgg
//
LOCUS       DAZFKM010000120        15125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFKM010000120 DAZFKM010000000
VERSION     DAZFKM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            25..1002
                     /gene="ybeQ"
                     /locus_tag="ACHKAY_003840"
     CDS             25..1002
                     /gene="ybeQ"
                     /locus_tag="ACHKAY_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO5702432.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPYYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1072..1554)
                     /gene="ybeL"
                     /locus_tag="ACHKAY_003841"
     CDS             complement(1072..1554)
                     /gene="ybeL"
                     /locus_tag="ACHKAY_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO5702433.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1789..4371
                     /gene="leuS"
                     /locus_tag="ACHKAY_003842"
     CDS             1789..4371
                     /gene="leuS"
                     /locus_tag="ACHKAY_003842"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO5702434.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4386..4967
                     /gene="lptE"
                     /locus_tag="ACHKAY_003843"
     CDS             4386..4967
                     /gene="lptE"
                     /locus_tag="ACHKAY_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO5702435.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4967..5998
                     /gene="holA"
                     /locus_tag="ACHKAY_003844"
     CDS             4967..5998
                     /gene="holA"
                     /locus_tag="ACHKAY_003844"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO5702436.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6000..6641
                     /gene="nadD"
                     /locus_tag="ACHKAY_003845"
     CDS             6000..6641
                     /gene="nadD"
                     /locus_tag="ACHKAY_003845"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO5702437.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6665..7276
                     /gene="cobC"
                     /locus_tag="ACHKAY_003846"
     CDS             6665..7276
                     /gene="cobC"
                     /locus_tag="ACHKAY_003846"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO5702438.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7536..7853
                     /gene="rsfS"
                     /locus_tag="ACHKAY_003847"
     CDS             7536..7853
                     /gene="rsfS"
                     /locus_tag="ACHKAY_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO5702439.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7857..8324
                     /gene="rlmH"
                     /locus_tag="ACHKAY_003848"
     CDS             7857..8324
                     /gene="rlmH"
                     /locus_tag="ACHKAY_003848"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO5702440.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8355..10256
                     /gene="mrdA"
                     /locus_tag="ACHKAY_003849"
     CDS             8355..10256
                     /gene="mrdA"
                     /locus_tag="ACHKAY_003849"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO5702441.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10259..11371
                     /gene="mrdB"
                     /locus_tag="ACHKAY_003850"
                     /gene_synonym="rodA"
     CDS             10259..11371
                     /gene="mrdB"
                     /locus_tag="ACHKAY_003850"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO5702442.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11382..12470
                     /gene="rlpA"
                     /locus_tag="ACHKAY_003851"
     CDS             11382..12470
                     /gene="rlpA"
                     /locus_tag="ACHKAY_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO5702443.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12610..13821
                     /gene="dacA"
                     /locus_tag="ACHKAY_003852"
     CDS             12610..13821
                     /gene="dacA"
                     /locus_tag="ACHKAY_003852"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO5702444.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13932..14195
                     /gene="ybeD"
                     /locus_tag="ACHKAY_003853"
     CDS             13932..14195
                     /gene="ybeD"
                     /locus_tag="ACHKAY_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO5702445.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14296..14937
                     /gene="lipB"
                     /locus_tag="ACHKAY_003854"
     CDS             14296..14937
                     /gene="lipB"
                     /locus_tag="ACHKAY_003854"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO5702446.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         3736 a         3844 c         3947 g         3598 t
ORIGIN      
        1 atctgcaacc ataaggaaat gataatgatt ttcacgtcaa gttgctgcga taatttatca
       61 atagatgaga ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata tattgttggg
      121 ttttattata atcgcgatag cgcaattgat tctccggacg acgaaaaagc cttttactgg
      181 ctgaagctgg ccgctgagca aggtcattgt gaagcacagt attccttagg gcaaaagtat
      241 accgaggata aaagccgtca taaagataat gagcaagcca tcttctggct gaaaaaagct
      301 gccctacaag gccatacttt cgcttccaac gcccttggct ggacactgga tcgtggagaa
      361 gccccctatt ataaagaagc ggttgtctgg tatcagatag ccgcggagag cggaatgtct
      421 tatgcgcaaa ataatcttgg gtggatgtac agaaatggca acggagtcgc aaaagactat
      481 gcgctggcat ttttctggta caaacaagct gcattacaag gccatagtga cgcgcaaaac
      541 aatctggccg atctttatga agacggaaaa ggcgttgctc aaaacaagac actcgccgca
      601 ttctggtatt tgaaaagcgc acagcagggt aatcggcacg cccagtttca aattgcatgg
      661 gattataacg ctggcgaagg ggtggaccag gactataagc aagcgatgta ctggtatctg
      721 aaggctgccg ctcaggaaag cgtcggcgct tacgtcaaca tcggttatat gtataaacac
      781 ggacaaggcg ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa agccgctgaa
      841 tgcaatgacg ccactgcctg gtataacctg gccattatgt atcactacgg agaaggaaga
      901 cctgtcgatc tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc cggaaccaga
      961 gatgtcagtc aggaaattcg tgagactgaa gatttactgt agagataaaa acaagggccg
     1021 gggaatttca ttcggcccat attctcttta ttgctgtgcg aaatcagact attacggctc
     1081 aaacgggcgt ctctggaact ggtcatgacc acatttcggg catagcgtca gcacttccgg
     1141 tgtgtagatc gggagatgga agtgacattt ctcgcagacc agatttccca gcccgaccac
     1201 ttctccgctg tgataaaccc catgatgatt gaggtcctgg aaaacttcgc gccattcaag
     1261 ctgcgtttta tcggtgatat ctgccagctc ctgccataag ctttctttaa tcacccgcat
     1321 aaagacgctg tcagattctt ctttcaggct ctcttcatag ctcatggcga actcttccag
     1381 gtcacgtctt acagctcgcg tcagctcatc gacctcggtt cgcgttaact cccctgtttt
     1441 tattacgcgc tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag
     1501 gcgttcgctc agtgacgcga ccagttcacg gtaatattga gcaaccttgt tcatcgtttc
     1561 atctcccggg tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat
     1621 acgcccacaa actctgtaaa ttaatgtatg aataatccgg gcatcatttc aacgtcgagt
     1681 gcgcgaaagg ctgttttgac gcggacgttt gggctatgct atgcggatct gaaaaaccac
     1741 ctcaacgcta catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa
     1801 taccgcccgg aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt
     1861 gaagtaaccg aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct
     1921 tctggtcgac tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc
     1981 taccagcgta tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc gtttggtctg
     2041 cctgcggaag gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac
     2101 atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag
     2161 ctggcaacct gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat
     2221 aaaaaaggcc tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc
     2281 gtactggcga acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa
     2341 cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac
     2401 gatctggata aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc
     2461 ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc
     2521 gtttacacta cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt
     2581 catccgctgg cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa
     2641 tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat
     2701 actggcttta aagctgttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac
     2761 ttcgtattga tggagtacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc
     2821 gactacgagt ttgcctctaa atacggcctg aacatcaaac cagttatcct ggctgctgac
     2881 ggctctgagc cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct
     2941 ggcgaattca acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact
     3001 gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgt
     3061 cagcgttact ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg
     3121 accccggacg accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc
     3181 agcccgatta aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcgctg
     3241 cgtgaaaccg acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact
     3301 tgcccagagt acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg
     3361 gatatctaca ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc
     3421 cacaaactga tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg
     3481 tgtcagggta tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac
     3541 tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg
     3601 aaagatgcgg caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag
     3661 aacaacggta tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg
     3721 tttatgatgt ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggtgtggaa
     3781 ggggctaacc gcttcctgaa acgtgtctgg aaactggttt acgagcacac agcaaaaggt
     3841 gatgttgcgg cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat
     3901 gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc
     3961 gcaattgcgg cgattatgga gctgatgaac aaactggcga aagcaccaac cgatggcgag
     4021 caggatcgcg ctctgatgca ggaagcgctg ctggccgttg tccgtatgct taacccgttc
     4081 accccgcaca tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac
     4141 gcgccgtggc cggttgctga cgaaaaagcg atggtggaag actccacgct ggtcgtggtg
     4201 caggttaacg gtaaagtccg tgccaaaatc accgttccgg tggacgcaac ggaagaacag
     4261 gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttaccgtg
     4321 cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag
     4381 gaagcgtgcg atatctggca acattgttgt tatctctggc tgtgttaatc accgccgggt
     4441 gtggctggca tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg
     4501 actcaggcga tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg
     4561 gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta
     4621 aagtgagcat cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc
     4681 agatgatcat gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc tacccgatta
     4741 gtgccaaagt cttccgttcg ttctttgata acccgcaaat ggcgttagcg aaagataacg
     4801 aacaagacat gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc
     4861 tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg accacaacgg
     4921 atactccggc aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta
     4981 cccggaacaa ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg
     5041 taacgatcct ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca
     5101 aggattcgaa gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt
     5161 ttcgttatgc caggctatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc
     5221 agaaaacgga ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca
     5281 tgacgacctg ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc
     5341 ctggtttact gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc
     5401 tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc
     5461 tgcaaatcag gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact
     5521 ggagcgttta tcgctgctct ggccagacgg caaattgaca ttaccgcgcg ttgaacaggc
     5581 ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa
     5641 aagtaagcgc gcgttgcata ttcttcagca actgcgtctg gaaggcagcg aaccggttat
     5701 tttgttgcgc acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc
     5761 ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat
     5821 gatgggcgag gcgttaaatc gcttaagtca gacgcagtta cgtcaggccg tgcaactcct
     5881 gacacgaacg gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga
     5941 agggttatct cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata
     6001 tgaaatcttt acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctta
     6061 aacccgtgga aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata
     6121 atgttcctcc gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg
     6181 aactggcgat tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg
     6241 ccccctctta cactgcgcaa acactgaaag agtggcggca ggaacaaggg ccggacgtgc
     6301 cgctggcgtt tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg
     6361 aaacgatact cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa
     6421 tggcgcaacc gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc
     6481 ttcaccttca gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg
     6541 cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac
     6601 cggtactgac ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg
     6661 catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa gcgaatatcg atggtcttta
     6721 cagcggtcat gcgcccaccc ccctgaccgc gcgcggtatt gagcaagcgc aaaatctgca
     6781 tacactgcta gatgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca
     6841 gcataccgcg cgacgggttc tcagtgaccg ccagctcccc gtgcaaatca tccctgaact
     6901 caacgaaatg ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga
     6961 tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga
     7021 aggatttcag gcattttcgc aacgtgtgga aagatttatc gcaaggctta gtgaatatca
     7081 gcactatcag aatattttaa tcgtcagcca tcagggcgta ctgagtctgt taattgctcg
     7141 tctaattggc atgcctgccg aatctatgtg gcattttcgc gttgaccagg ggtgttggag
     7201 cgccattgat atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg
     7261 ggtcgagaat gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc
     7321 cccgggcagg ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa
     7381 attgttttac aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc
     7441 gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac
     7501 catttttatc tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt
     7561 ttgttatcga caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg
     7621 gcaaatccag catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta
     7681 tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg
     7741 tagaaggtga aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg
     7801 tcatgcagga agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga
     7861 agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg
     7921 agtacctgcg tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa
     7981 aacgcggcaa gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg
     8041 cggccgcagg caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacgc
     8101 cgcagttagc cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga
     8161 ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc
     8221 tgtcggcgct taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc
     8281 gggcgtggag catcaccacc aaccatcctt atcaccgtga gtgataaagg tgctttgagt
     8341 agaaaacgca gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc
     8401 tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta
     8461 tcgccaacct gtataatctg caaattgttc gctttaccga ttaccagacc cgctctaatg
     8521 aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg cattatctac gaccgtaacg
     8581 gtatccctct ggcactcaac cgcactatct accagataga aatgatgccg gagaaagtcg
     8641 ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc gatgacgata
     8701 ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt cccgtgaaaa
     8761 ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg
     8821 tcgaagttaa aggctataaa cgtcgttact atccttacgg ttcggcgttg acccacgtca
     8881 tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac
     8941 tggccaacta tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag
     9001 acgtgctgca cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta
     9061 tccgccagtt aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc
     9121 tcaaactcca gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggtagtca
     9181 ccgatccgcg tacaggtggg gtgctggcac tggtttccac gcctagttat gacccaaacc
     9241 tgtttgttga cggtatctcc agcaaagatt attccgcctt gctgaacgac ccgaatacac
     9301 cgctggtgaa ccgcgccaca cagggggttt atcctcctgc gtctacagtt aaaccctatg
     9361 tggcggtttc ggcattaagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag
     9421 gctggtggca actaccaggg tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg
     9481 ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg
     9541 cctacgatat ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt
     9601 acaccggtat cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac
     9661 agaaacgctt taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggccagg
     9721 gttactggac agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg
     9781 gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga agacggcaaa caggtgccat
     9841 gggtacaacc gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga
     9901 aagacggtat gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcca
     9961 gcgcaccgta caaaattgcg gcgaaatctg gtaccgctca ggtcttcggt ctgaaagcga
    10021 acgaaaccta taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg
    10081 cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggcggcg
    10141 cgggtccggc ggttggtacg ctgatgcgcc agatcctcga ccacattatg ctgggtgata
    10201 acaataccga tctgcctgcg gaaaatcctg cggttgccgc agcggaggac cattaatcat
    10261 gacggataat ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct
    10321 gctgatctta ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca
    10381 ggatattggc atgatggagc gtaaaatagg ccaaatcgcg atgggtctgg tcatcatggt
    10441 ggtgatggcg caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat
    10501 ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gcgctcaacg
    10561 ctggctggac ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc
    10621 actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg ccatcgttga agaacactgg
    10681 catcgcgctg gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg
    10741 aacatcaatc ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg
    10801 gcgtctgatt ggcgtcgcag tagtgctggt ggcggcgttc attcctattc tgtggttctt
    10861 cctgatgcat gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc
    10921 actcggcgcg ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg
    10981 cggcaaaggc tggctgcatg gcactcagtc acaacttgaa tttctacccg aacgccatac
    11041 cgactttatc ttcgcggtac tggcggaaga gctgggatta gtgggcattc tgattctgct
    11101 cgctctctac attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac
    11161 ctttggtcgc gtcatggctg gcggcttaat gctgatatta ttcgtttatg tcttcgtaaa
    11221 tattggtatg gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta
    11281 tggaggatca gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac
    11341 ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg
    11401 ggatctgcat cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga
    11461 cggtaagtgt accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg
    11521 acccgcgttt cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa
    11581 gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg
    11641 atgccgaacc aggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagttga
    11701 cggcggccca tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg
    11761 ggcgaatgat cgtggtgcgc attaatgatc gcggccccta cggcaacgac cgcgtaattt
    11821 cgctttctcg cgcagcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg
    11881 atccgattat tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag
    11941 tcgccaaaca gacttacgcc ctgcctgcac ctcccgattt aagcggtggc gcgggggcaa
    12001 gttcagtgtc tggcccgcag ggtgacattc ttccggtcag taactcgacg ctaaaaagcg
    12061 aagatccgac cggcgcgccg gtaaccagta gcggtttcct cggcgctcca acgaccttag
    12121 cgcctggtgt actggaaagc agcgaaccga cacctgcacc acagcccgtt gttacagctc
    12181 cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg
    12241 ccagcggcaa ctttatggtg caagtcgggg ccgttagcga tcaggctcgt gcgcaacagt
    12301 accaacagca actgggacag aagttcggcg tccccggtcg cgtaactcaa aatggcgcgg
    12361 tctggcgtat ccagcttggc ccattcgcca gtaaagccga agccagtacc ttgcagcaac
    12421 gtttgcaaac cgaagcccaa ttacagtcat ttattaccac cgcgcagtag cgtaaagcag
    12481 gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc
    12541 ctgccggtag tgcatttgct atagtagggc acttttttta attccatcac ggatgtcgta
    12601 gttcagacca tgaataccat tttttccgct cgtatcatga agcgcctggc gctcaccacg
    12661 gctctttgca cagcctttat ctctgctgca catgccgatg acctgaatat caaaactatg
    12721 atcccgggtg taccgcagat cgatgcggag tcctacatcc tgattgacta taactccggc
    12781 aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc ctgctagcct gaccaaaatg
    12841 atgaccagtt acgttatcgg ccaggcaatg aaagccggta aatttaaaga aactgattta
    12901 gtcactatcg gcaacgacgc atgggccacc ggtaacccgg tgtttaaagg ttcttcgctg
    12961 atgttcctca aaccgggcat gcaggttccg gtttctcagc tgatccgcgg tattaacctg
    13021 caatcgggta acgatgcttg cgtcgccatg gccgattttg ccgctggtag ccaggacgct
    13081 tttgttggct tgatgaacag ctacgttaac gccctgggcc tgaaaaacac ccacttccag
    13141 acggtacatg gtctggatgc tgatggtcag tacagctccg cgcgagatat ggcgctgatc
    13201 ggccaggcat tgatccgtga cgtaccgaat gaatactcga tctataaaga aaaagaattt
    13261 acgtttaacg gtattcgcca gctgaaccgt aacggcctgt tatgggataa cagcctgaat
    13321 gtcgacggca tcaaaaccgg acacactgac aaagcaggtt acaaccttgt tgcttctgcg
    13381 actgaaggcc agatgcgctt gatttctgcg gtgatgggcg gacgtacttt taaaggccgt
    13441 gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt tcttcgaaac cgttaaccca
    13501 ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt ttggtgattc tgatcgcgct
    13561 tcgttagggg ttgataaaga cgtgtacctg accattccac gtggtcgcat gaaagatctg
    13621 aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc cgctgcaaaa gaatcaggtc
    13681 gtcggaacta tcaacttcca gcttgatggc aaaacgatcg agcaacgccc gctggttgtg
    13741 ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca ttgattacat taaattaatg
    13801 ttccatcact ggtttggtta aaaattgaac acttgaaagt gtaatttccg tccccatata
    13861 ctaagcatca gtaaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    13921 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    13981 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    14041 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    14101 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    14161 tgggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggctcct
    14221 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    14281 cacggagatg ccgttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    14341 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    14401 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    14461 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    14521 acttatcacg ggccgggaca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    14581 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    14641 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    14701 atttgctcac tgggtttacg aattcgacgc ggttgttcat tccacggtct ggcattaaac
    14761 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    14821 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc tccacgttta
    14881 ctggaaaata ttttagcgct actaaacaat ccggacttcg aatatattac cgcttaattc
    14941 catacatcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    15001 atctccttcg cgtttgtata ttttctttat cacttgcaga aatgcggtgc cacgccttgc
    15061 tccgcaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    15121 tccgt
//
LOCUS       DAZFKM010000121        15078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFKM010000121 DAZFKM010000000
VERSION     DAZFKM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            190..846
                     /gene="pphA"
                     /locus_tag="ACHKAY_003855"
     CDS             190..846
                     /gene="pphA"
                     /locus_tag="ACHKAY_003855"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO5702447.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(847..1038)
                     /gene="yebW"
                     /locus_tag="ACHKAY_003856"
     CDS             complement(847..1038)
                     /gene="yebW"
                     /locus_tag="ACHKAY_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO5702448.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1143..1379)
                     /gene="yebV"
                     /locus_tag="ACHKAY_003857"
     CDS             complement(1143..1379)
                     /gene="yebV"
                     /locus_tag="ACHKAY_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO5702449.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1497..2936)
                     /gene="rsmF"
                     /locus_tag="ACHKAY_003858"
     CDS             complement(1497..2936)
                     /gene="rsmF"
                     /locus_tag="ACHKAY_003858"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO5702450.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3016..5649)
                     /gene="yebT"
                     /locus_tag="ACHKAY_003859"
     CDS             complement(3016..5649)
                     /gene="yebT"
                     /locus_tag="ACHKAY_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO5702451.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5618..6901)
                     /gene="yebS"
                     /locus_tag="ACHKAY_003860"
     CDS             complement(5618..6901)
                     /gene="yebS"
                     /locus_tag="ACHKAY_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO5702452.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7031..7528
                     /gene="msrC"
                     /locus_tag="ACHKAY_003861"
     CDS             7031..7528
                     /gene="msrC"
                     /locus_tag="ACHKAY_003861"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO5702453.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7625..8323
                     /gene="proQ"
                     /locus_tag="ACHKAY_003862"
     CDS             7625..8323
                     /gene="proQ"
                     /locus_tag="ACHKAY_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO5702454.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8343..10391
                     /gene="prc"
                     /locus_tag="ACHKAY_003863"
     CDS             8343..10391
                     /gene="prc"
                     /locus_tag="ACHKAY_003863"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO5702455.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10583..11464
                     /gene="htpX"
                     /locus_tag="ACHKAY_003864"
     CDS             10583..11464
                     /gene="htpX"
                     /locus_tag="ACHKAY_003864"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO5702456.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11510..12883)
                     /gene="yebQ"
                     /locus_tag="ACHKAY_003865"
     CDS             complement(11510..12883)
                     /gene="yebQ"
                     /locus_tag="ACHKAY_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5702457.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13060..13851
                     /gene="kdgR"
                     /locus_tag="ACHKAY_003866"
     CDS             13060..13851
                     /gene="kdgR"
                     /locus_tag="ACHKAY_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO5702458.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13994..14233)
                     /gene="yobH"
                     /locus_tag="ACHKAY_003867"
     CDS             complement(13994..14233)
                     /gene="yobH"
                     /locus_tag="ACHKAY_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO5702459.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14392..14535
                     /gene="mgrB"
                     /locus_tag="ACHKAY_003868"
     CDS             14392..14535
                     /gene="mgrB"
                     /locus_tag="ACHKAY_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO5702460.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14610..14897
                     /gene="yebO"
                     /locus_tag="ACHKAY_003869"
     CDS             14610..14897
                     /gene="yebO"
                     /locus_tag="ACHKAY_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO5702461.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         3953 a         3663 c         3863 g         3599 t
ORIGIN      
        1 ctaaaagatt ttgttgtttg ttgacgaaaa caggaatcgt gttcggtctc tttttatctg
       61 ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac acactctact
      121 gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta gcgcgggagg
      181 aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga
      241 catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg
      301 cattgtcgtt ttgatccgtg gcgagattta cttatctcag taggagacgt tatcgatcgt
      361 gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggtttg tgcggtaaga
      421 ggcaatcatg aacagatggc gatggatgcg ctggcatccc agcagatgtc tttgtggttg
      481 atgaatggcg gcgactggtt tattgcgctg gcagataatc aacagaaaca agcgaaaacg
      541 gcgctggaaa aatgtcagca tttgcccttt attcttgaag tacacagtcg taccggcaaa
      601 catgttattg ctcatgccga ttatccagat gatgtttatg aatggcaaaa ggacgttgat
      661 ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg gcagggaatt
      721 acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt ggatattggc
      781 aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct tgtgcaattg
      841 caataattaa aaatcaccat actcctgtgc tggtcgccag aaaccatcta taaaatcctc
      901 aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa gacactgctg
      961 ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt agcaaacaaa
     1021 aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa gtttaggaga
     1081 gattttacaa cgggggaata accaggacaa ataacccgcc atgccgacgg gttctttttg
     1141 gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt acggtaaagg
     1201 acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca ggcatcgagt
     1261 tgcatacata aatcggggtc agatttacaa ggaatggtta acgttcgatc acccggcgat
     1321 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
     1381 ttcgtgctct ccatttagct gccgattgtg gtacgaccag tccgatcgca ccatttttca
     1441 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gagctgtcag
     1501 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
     1561 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caatacatca
     1621 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
     1681 tgcggtgtca gctcgaatgc attcatatta tcgggtgagg caagagcaat aaccgcttca
     1741 tgctgccagc gataaccttt gttgtgcgtc tcggcaagtt taatccccaa ccgagaaaat
     1801 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
     1861 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagtcgc ctgacgaatt
     1921 tgtccagctt cgcgatcttt caccgggctg aacggaaaat taccgacttt gtatttgggg
     1981 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
     2041 tcgtaaattt gtgggaaaac atgcaaaaag ccttcttcgg tcagcgcttt gtttgcacca
     2101 gggaagagat cgccaagtgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
     2161 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtacca
     2221 ccaggacgta atgcatgaaa ggcgctgtcg ataagctccc gttgtgtagc tgcgatttcc
     2281 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     2341 ccagagcagg gagcgtccag caaaatggca tcgaacattt ctggcactgc cgcaccaaac
     2401 acgcggccat caaaatgtgt gagcgcaaca ttactgatgc cacagcggct gatattggca
     2461 tgtaacactt ttacccgact ggcggaaaac tcattggcaa ggattgcccc ttcgttattc
     2521 atccgcgcgg caatttgcgt cgttttggag cctggcgcag ctgcgacatc catcacccgc
     2581 tgtggtgcat tatcgtcagc aaacaaggcg gcaacgggca acattgaact ggcttcctga
     2641 atataaaaca ggccgcttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     2701 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     2761 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     2821 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa atgagagcgt cgaaggcatc
     2881 gcttcgcgca tttgtgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     2941 atgtaccact ggcaaaaaaa caggcgcgca gtttagcata aacgctccgg cgcaggcacg
     3001 ccggagcagt gggcattatt tgggaagcgc agttccccat tcacgccact ctttcggttc
     3061 actttcctgc aacaggaagt gtttgccttc ctgggctttc ggtgccagtg gcgtacccgg
     3121 aggcgtggcg aaggcgatgc cgccacggat aaactggtta aaggtgccgg ttttcactac
     3181 gccgcccgtc agaccaaagt ccagactgta acccgatgcc aaccagaaga cggaattgtt
     3241 acgcaccagg tgttgatagc gtttactgat gcgcatcgca atcatcacgc gatctgacaa
     3301 tgtccccagc gtcattcctg taaccgtacc gacttccaga ccacggaaca gcacaggcgt
     3361 accaatacct aacgaaccgg cttccggcgc ttcaacaata atgcttaagc catccaggta
     3421 acgcgaatca gtaatggtgg cctcttgtaa ttcaaagtcg cggcgaggat tgccccggcc
     3481 tggttcgacg ttgatatacg gctggaggat agtatctaga tgctcaacgc ccgcggctga
     3541 aatttgcggt gtgaccaccg agaagcgcgt accgccgcgg gcgaaggtct ggacatactc
     3601 cggatagagc accgcctttg cctgtacttc attgcgagcg gtaatcagat ccagcgtctg
     3661 gatttgcccg atatcaatac cgagatagcg aattggcatc ccgaccgcca gttttccggc
     3721 atcgaaagcg tgaagcgtaa tctgcccacc aaccgcacgg gccgctgttt cggaagcata
     3781 cagaattcgt ttgtcgcctt tacgctgact ggcgctggca ccgctgaggt tatcgaagct
     3841 aatggctccc tttaatgctc tggagagcgg ggatgcctgt acggtcaggc cactaccatt
     3901 cagctgaact ttcgccccgc cttctgccca gaacacgcta ttgctggtca gaaggttgcg
     3961 atactccggc ttaatatgca gatcgatatc aaacgcgtta gctcgcggac ggacggtaat
     4021 aacttcacca acttcaaatt tacggtagag cactaccgat cctgcctgca catccggcag
     4081 cgtctctgca ctcaaactca cggttgtggt gggtaaatcg ctaaggctgt tctccagcgc
     4141 tttttccaga ttggcataca gtggatagct ggctttcatc tcgcctttat cgcccggcag
     4201 aatacgtatc ccgccgttaa tccattctga ggcgctggca ccgagaaact caacgccatc
     4261 cagccccacc ttcacgtcga cacggctgtt gacgacaaat ttgctatcgc cttttaccag
     4321 ttctcgatgc tgaggctcga tggcgacggt aaaggtgacg cctttgctgg tgagtttacg
     4381 atcgataacc tggcctacct gcacgccgtg aagaatgagc ggctgaccag catcaatacc
     4441 gtaactttcc ggcgcggtca gggtcagcgt cagaacatca ggttcatgca gcagtgcttt
     4501 ttcgcctggc acaacaacga actctttgcg tggctcgcca tcgccgggta ccaactcgaa
     4561 ggttttgcca gtcagcaggg cgctgagatt ggcatcgcta agggataatt tcggattacg
     4621 taattcgatg cgggtatttt cacgaagcag ggtaacgacg ctgggatcaa ctgtcatttc
     4681 cccagtgact ttaccaccag gatttaaatc cagtttagtt agctgtccga cttccagccc
     4741 ctgatacatt aacggcgtcg agtcggcggt caatccggcc ccactcggca gttccagttt
     4801 tattattacg ccacgctggc tgtgggccag atcttcatac agaccaaagg tatcttccgc
     4861 ctcggcaggt ttcgactctt caggtgaatc gaaggcaatc gcaccgttaa ccagtgccgc
     4921 cagactttcc agtttcacct tcgcgccact gacactgacg ttggcatcaa cgccggaaac
     4981 gttccagaaa cggctacctt ttttcaccag atcggtaaaa cgccgctcga tcaggacatc
     5041 aatcaccacg ccttgcttgt tgggattgat ggcatagtcg tagacttttc ccaccgggat
     5101 cttgcggaaa tagaccaatg aaccgctgtt cagcgaaccg agatcgggag cttgcaggtg
     5161 gatcatcaga tcgccgttat ccagccgata cttcggttgg gtatcgagtg cgacaaagtg
     5221 atcctgctct ttacctttac ccggcatcat gccgatatag ttcccaccga cgagggcgtc
     5281 cagcccggag acacctgcca acgatgcttt tggcgtcacc agccagaatt gagtctcttc
     5341 gcgcagcgca tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc
     5401 gtcgctgagg ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt
     5461 acggcccgga acaataccat ccgccgacat aaagtcgatg gtgacggtat taccccggtc
     5521 ctgataactg tcccaaatca gccaaccggc aatcattagc gcgatgaaag gcagcagcca
     5581 gaaaggtgag atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc
     5641 ctgactcatg tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca
     5701 aaattacggc tgcgccaaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat
     5761 cgcgattaat cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgtc
     5821 caatccaggt caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa
     5881 aatgaatgct caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac
     5941 tggcgataaa cacgattccc gcaaccgcaa tgttgctact tgccagcgac ataattccgg
     6001 aaagaattgt atcttcctgc cgtcctccgt tcagataaat gatagaaata ggcaataggt
     6061 tggctggtaa caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcagactat
     6121 gacggcgacg caggcgtagc gggatatggc aacgcgggca gcgaccacgc tgatcaggat
     6181 agccggtaaa atggcaccca agacagacac gaagtttctc gtccctacgc gtagcggggc
     6241 gctgcggata atatcgctcc cacagctcct caacattaag atgtgacaac gtcaccgtcg
     6301 ttaaaatcac caacgccaca aaagagaaca agccgacacc agcctggata tgggcataat
     6361 cctgtacctt tatagaagca acgccaatgc cgaccaggta gatatccagc attacccact
     6421 ctttaagacg ctcaagcatc agcagtaccg gacgtaaatt cattcccagt cggttaccaa
     6481 accataaata tgctatggag gacaccagaa tgaggggggc accaataacg cagaaaaaga
     6541 ccatcgcccc cgttatcgta tcgccctgtt tggtcatttg ccagatgcct tgcatgacgt
     6601 tggcgtcgat acgaatacct aacagccaga tatgcaacag cggttcgccc caggcaaacg
     6661 gcatcagcaa cagcatggtg aaagccattg ccgccaggcg cgttagcgac cagtcgcgcc
     6721 cgtcgcgaat ttttgcctga cagcgcggac aataggcact ctgatgagaa tttatctcgg
     6781 gcaggctaaa cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct
     6841 cgccgattgc cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca
     6901 tgacgtgcca aatgtaataa gaattaacat taatattaac ccatgattgg aatgatcttg
     6961 agcattaacg catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaccaag
     7021 tgattatata atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct
     7081 gatggcggga gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatacga
     7141 gcgtctcact gacgtaaact gggcaggttt ttatttgctt gaggacgata cactggtact
     7201 aggaccattt cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gggtgtgcgg
     7261 cactgcggtt gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt ttgacgggca
     7321 tattgcctgt gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca
     7381 gattattggt gttcttgaca tcgatagtac cgtcttcggt cgctttacag acgaggacga
     7441 gcaaggctta cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa
     7501 aaaattcttt gcgagcgtcg caggataatc aacggataac gtagcattta ctggtagcgt
     7561 cattataatg acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa
     7621 tttcatggaa aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctggccga
     7681 acgttttccc cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa tcggtatttt
     7741 tcaggatttg gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc
     7801 cgctttacgt ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac
     7861 gcgtgtcgat cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc
     7921 tcgcaagcag cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaagc
     7981 gaaaaaacgc gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc gccgcgaacg
     8041 caagccacgt ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca
     8101 aaagccggta gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac agcacacccc
     8161 agtttctgac atttcagctc tgactgtcgg acaagccctg aaggtgaaag cgggtcaaaa
     8221 cgcgatggat gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc
     8281 gggtatgtct ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag
     8341 gcatgaacat gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga
     8401 ccttcgctgt agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc
     8461 agcatgcgac ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt
     8521 tcgacctcga tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt
     8581 acagccacaa cgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt
     8641 taggcgatga actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc
     8701 aaaagcgccg ttttgagcgt taccagtacg ctttgtcggt actggaaaag ccgatggatt
     8761 tcaccggcaa cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg
     8821 ctgagttgaa cgcgctgtgg gacagtaaag tcaaattcga cgagttaagc ctgaagctgg
     8881 caggaaaaac ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc
     8941 gtcgtctggc gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg gcgtttgcgc
     9001 gtgaaatcga cccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg
     9061 aaatgagttt gtcgctggaa ggtattggcg cagtgctgca aatggatgat gactacaccg
     9121 ttatcaattc gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg
     9181 acaaaattgt cggtgttggt caaacaggca agccgatggt tgacgtgatt ggctggcgtc
     9241 ttgatgatgt ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt
     9301 tacctgctgg taaagggacc aagacccgca ctgtaacatt gacccgtgaa cgtattcgtc
     9361 tcgaagaccg cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa gtcggcgtgc
     9421 tggatattcc gggcttctat gtgggtttga cagacgatgt caaagttcaa ctgcagaaac
     9481 tggaaaaaca gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt
     9541 tgaccgaagc ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt gttcaggtcc
     9601 gcgataacaa cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg ttctataaag
     9661 gcccgctggt ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc tttgccgcgg
     9721 caatgcagga ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg
     9781 ttcagcaata ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag
     9841 cgctgggttc tgtgcagtac acgatccaga aattctatcg cgttaacggc ggcagtacgc
     9901 aacgtaaagg cgtaacgcca gacatcatca tgccgacggg taatgaagaa acggaaacgg
     9961 gtgagaaatt cgaagataac gcgctgccgt gggatagcat tgatgccgcg acttatgtga
    10021 aatcaggcga tttaacggcc tttgggccgg agctgctgaa ggaacataat gcgcgtatcg
    10081 cgaaagatcc tgagttccag aacattatga aggatatcgc gcgcttcaac gctatgaagg
    10141 acaagcgcaa tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat aatgaagatg
    10201 atgcgacgcg tctggcgcgt ttgaacgaac gctttaaacg cgaaggtaaa ccggagttga
    10261 agaagctgga tgatctaccg aaagattacc aggagccgga tccttatctg gatgagacgg
    10321 tgaatatcgc actcgatctg gcgaagcttg aaaaagccag acccgcggaa caacccgatc
    10381 ccgtcaagta atatcaatca ggcacaagaa attgtgcctg attttttaac agcggcaaga
    10441 tgccgtaaat cagatgctac aaaatgtaaa gttgtgtctt tctggtgact tacgcactat
    10501 ccagacttga aaatagtcgc gtaacccata cgatgtgggt atcgcatatt gcgttttgtt
    10561 aaactgaggt aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa cgaacctggc
    10621 cgtaatggtc gttttcgggc tggtactgag cctgacaggg atacagtcga gcagcgttca
    10681 ggggctgatg atcatggcct tgctgttcgg ttttggtggt tccttcgttt cgcttctgat
    10741 gtccaaatgg atggcattac gatctgttgg cggggaagtg atcgagcaac cgcgtaacga
    10801 aagggaacgt tggctggtca atactgtagc aacccaggct cgtcaggcag ggatcgctat
    10861 gccgcaagtg gctatctacc atgcgccgga catcaacgct tttgcaaccg gtgcgcgccg
    10921 tgatgcctct ctggttgctg tcagcaccgg tttgctgcag aacatgagcc cggatgaagc
    10981 cgaggcggta attgctcacg aaatcagtca catcgccaat ggtgatatgg ttaccatgac
    11041 gctgattcag ggcgtggtga acaccttcgt tatctttatt tcccgtattc tggcgcagct
    11101 tgccgcgggt tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg gcaacccgct
    11161 gatctacttt gcggttgcaa cggttctgga actggtgttt ggtattctgg cgagcattat
    11221 caccatgtgg ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg caaaactggt
    11281 tggtcgcgag aaaatgattg ccgcgctgca gcgcctgaaa accagctatg aaccgcaaga
    11341 agcaaccagc atgatggctt tctgcattaa cggtaagtcg aaatcgctca gtgagttgtt
    11401 catgactcac ccgccgctgg ataaacgaat tgaagctctg cgtacgggtg aatacctgaa
    11461 gtaatatgaa gtaaatacta aaaggcgcgt cctgatcgac gcgctttttt tatgccctgg
    11521 atcgtggctg agtgatacgt aaaccactca cacaggcagc aatcactgcc agaatagccg
    11581 cagccatcag cgaaacgtgc gtaccattat caccgaactg atttagcatc agcgccacca
    11641 gcgccgcgcc actactctga cccagtagac gagccgttcc taacatgcca ctggccccac
    11701 cgctacgttc gcgcggagcg gaggtaataa tggtgtggtt attgggtgac tggaataagc
    11761 caaatccagc gccacataag atcatcggcc agataatatt gatatccgca ggtgacgcgg
    11821 gcagcagaac cagggaaaaa agccccgcag ccatgatgaa caaccctaaa gcacccagca
    11881 atcctgcatg tacgcgttca atcaaatagc ctgccagcgg agccatcacc atcgttgcta
    11941 acggccacgg tgtcagaagt aaacctgttt cgacttcact acgcccgagc acggtttgca
    12001 ggtaaaaggg cagggaaacc attgccagca tttgtgcgca gaaagagcaa acagatgtgc
    12061 aaatagaaag tgaaaacagc gggatacgca gtaaatccac cggtagcagc ggtacgggaa
    12121 gagaaagctg gcggcgaata aagaaaatac caacaacaac cattaccacc agttccgcag
    12181 caattaacgt cagcgattgc ccctgagcga aaccactcaa cgcagtgata agcaggccga
    12241 aggttaacgc gttcatcacg gcgctgggca ggtcgaaacg gggtttactg gcgcgagaac
    12301 cattgggtgg cagaaaacgc atcgccagaa gcagggcgat aatacctaac ggtacgttga
    12361 ttaaaaataa ccatttccag gatgcgatgg agaggattgc tgcagcaatt gtcggcccgg
    12421 cagcagaaga aacggcaaca ataaacgagt ttatgcccat ccctctcccc agaaaacgtt
    12481 gtggatagat caggcggata agtgcggtat taacgctcat caacgctgca ccgccgaaac
    12541 cttgtatgac acgcgcaagg gtgagcattt gcagcgaatc agaaagggcg cagaacagtg
    12601 aagacaacag aaaaacgacc agaccgcatt tataaatacg tcgatagcca aacatatcgc
    12661 ccagaaacga aaacgagagc agggagatga caatggcgat ttgataggcg ttcactaccc
    12721 agatggatct ggctggcgtg gcatgaaggt ccgtggcgat tgttggcagg gcgacgttgg
    12781 cgattgcgcc gtcaaggaca gccatcgaaa tgccaatcac aatggttaat atcgcaccgt
    12841 atcgctgggg caatggcagg ccgtcggcct gaacttttgg cataggaatt ttatatcttt
    12901 ggtgaataat caacaggggt atgattctag cactgatatt cacggtggat gtcgcagaat
    12961 tgtaactaac cggcaaaaga tggattgcga ccaccctggt gcgaaattat aataaaaaac
    13021 agttctgatt tttataaaac actcgcaatg aggtgataaa tggctaacgc agatctggat
    13081 aaacagcctg attctgtatc ttccgtgcta aaagtttttg gcattttgca ggcgctgggt
    13141 gaagagcgcg aaatagggat aaccgagctg tcgcagcgcg tcatgatgtc aaaaagcacc
    13201 gtttatcgct ttttacagac catgaaaacc ttaggttatg tggcgcagga aggggagtcg
    13261 gagaaatatt ccctgaccct gaaattgttt gaactgggcg ctcgcgcgtt acaaaacgtc
    13321 gatttaattc gtagcgcaga tatccagatg cgtgagatct cccgcctgac caaagaaact
    13381 atccacctcg gcgcactgga cgaagacagt attgtttaca ttcacaaaat tgactctatg
    13441 tacaatttgc gcatgtattc acggattggg cgtcgtaatc cgctgtacag caccgcgatt
    13501 ggtaaggtac tgctggcatg gcgcgatctc gatgaagtga agcaaattct tgagggcgtg
    13561 gagtataaac gcagtaccga gcggaccatc accagtacag aagcgttatt acccgttctg
    13621 gaccaggtgc gcgagcaggg gtatggcgaa gataacgaag agcaggaaga agggctgcga
    13681 tgcattgcgg taccggtatt tgatcgcttt ggcgtggtca ttgccggttt gagcatctcc
    13741 ttcccgacgt tgcgtttctc tgaagagcgt ttacaggaat atgtcgcaat gttgcatacc
    13801 gcagcgcgca aaatttctgc ccaaatgggt tatcacgact atccgttctg atgagagtaa
    13861 gaacctgtcg gaatatcaaa cagacaggtt ctttatttag catgagaaaa ataaagttga
    13921 aggtgcgtta tattaaacgc gcttgctata agaatatttt actcatgagt gagaatctgg
    13981 ttatttatta cccttaacca ttatcgacca cgatattgct tttacgtaac agtgggcaat
    14041 ctgttatccc caagaaacca cttttagtgt gcaagtattg taccgtgctg gtgcctctgg
    14101 cagtcagata ggtacattgc aaacctaatc ctgcggcatt ctctttgctt ccaatcaaaa
    14161 cgccatatcc gctgagtaat aatcctatcc ataccagtgc tatcagcata actgtgcgaa
    14221 tgatgaatcg cattacaacc tcttctcttt ttatgttcgc ttaatcgtag cggcaatatg
    14281 cgctgaagca agctactcat tccgaaaaag cacgaatatc gacatagtta ggcgctgttt
    14341 aactaacgca tgctagttta atgacataag gtaggtgaag cggagattgg agtgaaaaag
    14401 tttcgatggg tcgttctggt tgtcgtggtg ttggcttgct tgctgctttg ggcgcaggta
    14461 ttcaacatga tgtgcgatca ggatgtacaa tttttcagcg gaatttgtgc cattaaccag
    14521 tttatcccgt ggtgatatca ttttttcagc actagatttt cttcctctcc agcagtggta
    14581 gaatagcgcc tctactcaga ggtggtgaaa tgaacgaagt tgtaaattca ggcgtgatga
    14641 acattgcgtc tttggttgta tcggtggtgg ttcttcttat cgggctcatc ttgtggtttt
    14701 ttatcaatcg tgccagttct cggactaacg aacagattga actgcttgag gcgttgctgg
    14761 atcagcaaaa acgtcaaaat gcactgttac gtcgtttgtg cgaagcaaac gaacctgaga
    14821 aagcagataa aaagaccgtt gagagtcaaa aatcggttga agacgaagat attattcgtc
    14881 tggtcgccga acgataatcc ttcctcaatc ttgagtaacc ccgtcgttga agggttactc
    14941 aattcatcct gattatacgc actgccatgg tgcaactgct tcaaattaaa actctggtct
    15001 gacttcaaat aattatcata ttatttattt gcgtaacggg atatcagaaa aagatatttt
    15061 cctcattaat ttattagg
//
LOCUS       DAZFKM010000122        15060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFKM010000122 DAZFKM010000000
VERSION     DAZFKM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..411
                     /gene="tnpA"
                     /locus_tag="ACHKAY_003870"
     CDS             <1..411
                     /gene="tnpA"
                     /locus_tag="ACHKAY_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO5702462.1"
                     /translation="KETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEKLRTYFSN
                     VCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAVRYY
                     YKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(427..615)
                     /locus_tag="ACHKAY_003871"
     CDS             complement(427..615)
                     /locus_tag="ACHKAY_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO5702463.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(619..978)
                     /gene="yeaR"
                     /locus_tag="ACHKAY_003872"
     CDS             complement(619..978)
                     /gene="yeaR"
                     /locus_tag="ACHKAY_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO5702464.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1151..1789)
                     /gene="leuE"
                     /locus_tag="ACHKAY_003873"
     CDS             complement(1151..1789)
                     /gene="leuE"
                     /locus_tag="ACHKAY_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO5702465.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1916..2839)
                     /gene="dmlR"
                     /locus_tag="ACHKAY_003874"
     CDS             complement(1916..2839)
                     /gene="dmlR"
                     /locus_tag="ACHKAY_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO5702466.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2942..4027
                     /gene="dmlA"
                     /locus_tag="ACHKAY_003875"
     CDS             2942..4027
                     /gene="dmlA"
                     /locus_tag="ACHKAY_003875"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO5702467.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4278..5888
                     /gene="yeaV"
                     /locus_tag="ACHKAY_003876"
     CDS             4278..5888
                     /gene="yeaV"
                     /locus_tag="ACHKAY_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO5702468.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5920..7044
                     /gene="yeaW"
                     /locus_tag="ACHKAY_003877"
     CDS             5920..7044
                     /gene="yeaW"
                     /locus_tag="ACHKAY_003877"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO5702469.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7100..8065
                     /gene="yeaX"
                     /locus_tag="ACHKAY_003878"
     CDS             7100..8065
                     /gene="yeaX"
                     /locus_tag="ACHKAY_003878"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO5702470.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8119..9234)
                     /gene="rnd"
                     /locus_tag="ACHKAY_003879"
     CDS             complement(8119..9234)
                     /gene="rnd"
                     /locus_tag="ACHKAY_003879"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO5702471.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9316..11001)
                     /gene="fadD"
                     /locus_tag="ACHKAY_003880"
     CDS             complement(9316..11001)
                     /gene="fadD"
                     /locus_tag="ACHKAY_003880"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO5702472.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11206..11787)
                     /gene="yeaY"
                     /locus_tag="ACHKAY_003881"
     CDS             complement(11206..11787)
                     /gene="yeaY"
                     /locus_tag="ACHKAY_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO5702473.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11827..12522)
                     /gene="tsaB"
                     /locus_tag="ACHKAY_003882"
     CDS             complement(11827..12522)
                     /gene="tsaB"
                     /locus_tag="ACHKAY_003882"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO5702474.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12580..14490)
                     /gene="yoaA"
                     /locus_tag="ACHKAY_003883"
     CDS             complement(12580..14490)
                     /gene="yoaA"
                     /locus_tag="ACHKAY_003883"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO5702475.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14622..14966
                     /gene="yoaB"
                     /locus_tag="ACHKAY_003884"
     CDS             14622..14966
                     /gene="yoaB"
                     /locus_tag="ACHKAY_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO5702476.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3666 a         3979 c         3721 g         3694 t
ORIGIN      
        1 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
       61 tttgtcacca gataccgact ccagattttt gaccatgacg cgacagaaaa actacgcact
      121 tacttttcaa atgtatgtgc tgattttgaa gctgaacttg ttgaaatgga tggcgaacca
      181 gatcacgtcc atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac
      241 agcctcaaag gcgtatcggg taggttactg cgacgagatc gaccagatat tgcagtcagg
      301 tattactaca aaggcgtttt gtggagtcct ggctattttg ccagtagctg cggaggtgcg
      361 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtta gatggaaaac
      421 cgcgccttat atccccgccc tgaaggacgg ggttttacgg cgcaccggat aagaacgcac
      481 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
      541 cgtctctgtt tcataatcga cagaaacgcc attgctgtta ttggtgacgg tcacggtata
      601 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
      661 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
      721 ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat
      781 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
      841 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
      901 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
      961 ctgtgggatt tgcagcatcc tattccctcc ttcggggaat gcgctgaaaa cggtttattc
     1021 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     1081 tagcaccata tcagagaaaa atcagggagg attacaagtg gggagcccgc gacaacgcgg
     1141 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     1201 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     1261 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     1321 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     1381 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     1441 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     1501 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     1561 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     1621 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     1681 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     1741 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     1801 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     1861 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaatc attaatcagg
     1921 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctaccg
     1981 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     2041 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     2101 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     2161 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     2221 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     2281 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     2341 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     2401 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     2461 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     2521 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     2581 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     2641 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     2701 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     2761 ccgccacggc ggcaaaacca gcccgacgag ccaccagcat aaagacgcgc aaatcattca
     2821 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     2881 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     2941 aatgatgaaa acgatgcgta ttgctgcgat cccgggagac gggattggca aagaagtcct
     3001 tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc tggggcttcg ccttgagttt
     3061 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
     3121 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
     3181 ggacaccgtt cctgaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
     3241 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
     3301 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
     3361 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
     3421 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
     3481 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
     3541 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
     3601 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
     3661 ggtggcgtcc aatttatttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
     3721 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
     3781 gccagtccac ggttccgcgc cggatatcta cgggaaaaac attgctaacc ctatcgccac
     3841 gatttgggcc ggtgcaatga tgctcgattt tctcggcaat ggcgatgagc gtttccagca
     3901 agcgcataac ggtattctgg cagcaattga agaagtgatt gctcacgggc cgaaaacacc
     3961 tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
     4021 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
     4081 tttgcagtaa ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
     4141 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
     4201 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
     4261 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
     4321 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
     4381 ccgacgcggc caacgcaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
     4441 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
     4501 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
     4561 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
     4621 aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt agcgtccctt
     4681 actccttctt ccactggggc atcagcgcct gggcaactta tacgctggcc tcattaatca
     4741 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
     4801 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgttcctga
     4861 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
     4921 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
     4981 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
     5041 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggcc
     5101 caaccgaatt tattaccaac agcatcatca atgccatcgg tctgaccacg caaaacttcc
     5161 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
     5221 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
     5281 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
     5341 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
     5401 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
     5461 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
     5521 tccttgcctc gcatatggat gcggtggcct acaccatggc tgcgaccagt acgcgtaatc
     5581 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
     5641 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
     5701 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
     5761 ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
     5821 caccggttga agccctggaa aaaacgccag tgctccctgc gggaaccgta ttcaaaggcg
     5881 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
     5941 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
     6001 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
     6061 atttgcgtcg ctcacagcat tgaactggcg aatgccaatg attatgtgac gcgtgagatc
     6121 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
     6181 gtgtgtccgc accgtggtca tcagttgttg agcggtgaag gaaaagcaaa aaatgtgatt
     6241 acctgcccgt atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
     6301 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
     6361 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
     6421 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
     6481 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
     6541 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgt
     6601 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
     6661 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
     6721 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
     6781 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
     6841 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
     6901 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
     6961 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
     7021 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
     7081 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
     7141 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
     7201 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccagta
     7261 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
     7321 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
     7381 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
     7441 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagct
     7501 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
     7561 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gttcacttgc atcactcatc
     7621 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
     7681 ttatacctgt ggtcccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
     7741 catcgtcgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
     7801 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
     7861 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
     7921 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
     7981 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
     8041 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
     8101 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
     8161 catcagctca ccacgccagc cggaaatcag cgccggcaaa ttgttctgcg gtttcagttt
     8221 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
     8281 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaaca ctttacgata
     8341 accaggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
     8401 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
     8461 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
     8521 ctcttcacgc acgacaaagt tcaccgccag atcacgctct cgcgccttgc gcagtcgcca
     8581 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aactgccagg cattggtgat
     8641 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
     8701 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
     8761 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
     8821 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
     8881 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
     8941 gtcaatcaag ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
     9001 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
     9061 gtcggtgatc ccgagaggat caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa
     9121 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg
     9181 aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
     9241 attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
     9301 cgactgactt aacactcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
     9361 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
     9421 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
     9481 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
     9541 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
     9601 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
     9661 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
     9721 ctcatctgta gcatccggac gctgccagta acccagcatc acctgcggtc ctttgacaca
     9781 aagctcaccc ggttgacccg gtggtacttc attatcatca tcatccacca gtttggcttc
     9841 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
     9901 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtcctgtcag
     9961 tttcacccaa cgctctgcca ccacttgctg cactggcatc cctccgcctg cggaaagatg
    10021 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    10081 gttaacgccc gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    10141 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    10201 aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc accaccagct ctttgcccgg
    10261 atgcaacagc ggaccatagg tcgcgttaac ctgttccagg ttcgccagca tattgcggtg
    10321 agtcagcatc gcgcctttcg ccacaccagt ggtgccgccg gtgtattgca gaaaagctaa
    10381 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    10441 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    10501 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    10561 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    10621 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    10681 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    10741 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    10801 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    10861 ctcccccata ttcacaaacg caggttgatc ggcgtagcgc gcgaccgact gctcaaacat
    10921 atctaccaga gattgataac ggtcagggtt gatctccgtc ggaacgtccg cgggataacg
    10981 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    11041 cccaaatgat atacatgccg ttaacataat attaactcat cataccagct tgataattac
    11101 ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta tctttaccgt aagaatgcag
    11161 aaacagcgga ccagccgctg tttctttttc tttaaaaaca agcaattatt cagttacaac
    11221 tgtttgtact ctcgcggggc cgggattata ccagccccat ccgccgtagc catagggcca
    11281 gccacgaccg ccataaaacc atggatcaat cggctgaggc ggcataatca cctgctgggt
    11341 taaatgccaa cgtttgtaac ccgttacttg catcaccata aatttatagg gcgtattgcc
    11401 gattttgccg tcaaccgcac cagtgattgg cccgactacc gtaacaagtt gtccacggaa
    11461 atccaccggg tccagaaaac cgttcacatc ggcataaatg cgaccgcgag aaggttctcc
    11521 cagcgtcggt ctggctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    11581 ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt gcctcctgac caacgtacag
    11641 ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc gtgggactgc tgcctttaat
    11701 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    11761 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    11821 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    11881 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    11941 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    12001 accgagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    12061 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    12121 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    12181 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag
    12241 cgtggagacg ccaatcatcg gtagctctgc gccaagtgcc agcccttgcg caatgccaat
    12301 accaatgcgc acgccggtaa agctaccggg gccgcgcccg taaaccagag cgttaatatc
    12361 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    12421 atgttcacga gggcaaagct caaaatgagc gttgacagta ccgtcgttcc acagggcgac
    12481 agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    12541 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    12601 aaggaaacga accgcacggg caatgtcacg agtgcgtggc gcgggcggca gactggcgag
    12661 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaatcacca gcacgccacg
    12721 atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg agagtaatga cggcatctgg
    12781 tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    12841 taacagtgga tcatccggcg aggtaaacgg caacttgtcg ataatcacca atgacaatgt
    12901 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    12961 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    13021 aagcgtcatg gtagcgcgga actgctcggc cagatcacgc atcatggcgt gcgaggtaca
    13081 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgctaactg
    13141 gcgagcagaa cctggctggt tggtttgcgg cagattgcgc ggcacacaga gtaacgcctg
    13201 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    13261 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    13321 accaggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    13381 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    13441 ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc tcaaatgccg catccagcaa
    13501 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    13561 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    13621 acctggctca ccgagttgca gacgaaaatc ctgcgcactc tgggcaagac gatcagcgca
    13681 cttttgtaac tgctgggtgt cttttaattc ggtgcggtaa gcgatggtga tgtcttttgc
    13741 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    13801 tagctggtgg gcttcgtcga agatcatgac gtccgcttcc gggatcagtt cgccaaatcc
    13861 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    13921 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    13981 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    14041 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    14101 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    14161 ctcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    14221 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    14281 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    14341 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    14401 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    14461 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    14521 tgtcaggatg agggcgcttt cgccagttga attggtgacg gcgacctcac attgtggcag
    14581 tctttgcagc acgaaaatgg aaggataatg aggacaaaat gatgactatc gttcgtatcg
    14641 atgctgaagc ccgctggtct gatgtggtaa tccacaacaa cacgctctac tacactggtg
    14701 taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc caacacgctg gcgcagattg
    14761 atgccgtgct ggaaaaacag ggcagcaata aatcgagcat tctggatgcc accattttcc
    14821 tggccgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtcgcgg
    14881 gtcatgcgcc ggtgcgctgc acggtacaag cgggtttgat gaacccgaag tataaagttg
    14941 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    15001 tatcgataat actggtcggt tttacataaa tcgaccagag attaagatat tacctgttga
//
LOCUS       DAZFKM010000123        15047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFKM010000123 DAZFKM010000000
VERSION     DAZFKM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..370
                     /gene="yjbE"
                     /locus_tag="ACHKAY_003885"
     CDS             128..370
                     /gene="yjbE"
                     /locus_tag="ACHKAY_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO5702477.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            484..1122
                     /gene="yjbF"
                     /locus_tag="ACHKAY_003886"
     CDS             484..1122
                     /gene="yjbF"
                     /locus_tag="ACHKAY_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO5702478.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1119..1856
                     /gene="yjbG"
                     /locus_tag="ACHKAY_003887"
     CDS             1119..1856
                     /gene="yjbG"
                     /locus_tag="ACHKAY_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO5702479.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1856..3952
                     /gene="yjbH"
                     /locus_tag="ACHKAY_003888"
     CDS             1856..3952
                     /gene="yjbH"
                     /locus_tag="ACHKAY_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO5702480.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3999..4277)
                     /locus_tag="ACHKAY_003889"
     CDS             complement(3999..4277)
                     /locus_tag="ACHKAY_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702481.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4278..4421
                     /locus_tag="ACHKAY_003890"
     CDS             4278..4421
                     /locus_tag="ACHKAY_003890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702482.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4547..4957
                     /gene="psiE"
                     /locus_tag="ACHKAY_003891"
     CDS             4547..4957
                     /gene="psiE"
                     /locus_tag="ACHKAY_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO5702483.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5001..6476)
                     /gene="xylE"
                     /locus_tag="ACHKAY_003892"
     CDS             complement(5001..6476)
                     /gene="xylE"
                     /locus_tag="ACHKAY_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO5702484.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6848..7738)
                     /gene="malG"
                     /locus_tag="ACHKAY_003893"
     CDS             complement(6848..7738)
                     /gene="malG"
                     /locus_tag="ACHKAY_003893"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO5702485.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7753..9297)
                     /gene="malF"
                     /locus_tag="ACHKAY_003894"
     CDS             complement(7753..9297)
                     /gene="malF"
                     /locus_tag="ACHKAY_003894"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO5702486.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9451..10641)
                     /gene="malE"
                     /locus_tag="ACHKAY_003895"
     CDS             complement(9451..10641)
                     /gene="malE"
                     /locus_tag="ACHKAY_003895"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO5702487.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11006..12121
                     /gene="malK"
                     /locus_tag="ACHKAY_003896"
     CDS             11006..12121
                     /gene="malK"
                     /locus_tag="ACHKAY_003896"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO5702488.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12193..13533
                     /gene="lamB"
                     /locus_tag="ACHKAY_003897"
     CDS             12193..13533
                     /gene="lamB"
                     /locus_tag="ACHKAY_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO5702489.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13776..14696
                     /gene="malM"
                     /locus_tag="ACHKAY_003898"
     CDS             13776..14696
                     /gene="malM"
                     /locus_tag="ACHKAY_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO5702490.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14925..>15047
                     /locus_tag="ACHKAY_003899"
     CDS             14925..>15047
                     /locus_tag="ACHKAY_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702491.1"
                     /translation="MISHILNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVPGLN"
BASE COUNT         3731 a         3870 c         3956 g         3490 t
ORIGIN      
        1 tccaattatc ctaaaacgcc atcgctaatt ccccgcgccg taattcgcat gctttagttg
       61 tgtatactcg atcccgcccg aaatgttttt gggtaaatct ccattcattc aatgaaggga
      121 aattgttatg aaaaaagttc tgtatggcat ttttgccata tctgcgcttg cggcgacttc
      181 tgcgtgggct gcacctgtac aggtgggcga agcggcaggg tcggcagcaa cgtcggtttc
      241 ggcggggagt tcctccgcta ccagcgtcag caccgtaagc tcggcggtgg gtgtcgcgct
      301 cgcggcaacc ggtggcggtg atggttctaa taccgggacc accacaacca cgaccaccag
      361 tacccagtaa taaagtatgt atccccaaaa taatttgagt cattgcatct gtggcttgaa
      421 gtatgaaggg atttaccata accacactcc ggtgtggtta ttctgcccct ctggagaaga
      481 gtcgtgaagc gacctgcact cattcttatc tgcctgctat tacaggcctg ttcagccacg
      541 actaaagagc tgggcaattc actgtgggac agtctgttcg gcacgccagg cgtacagctg
      601 acggacgatg atattcaaaa tatgccctac gccagccagt acatgcagct taatggcggg
      661 ccgcaattgt ttgtggtgct ggccttcgct gaagacggac aacaaaaatg ggtcactcag
      721 gatcaagcca ctcttgtcac acaacatggt cgtctggtga agactttgct tggcggcgac
      781 aacctgattg aagtgaataa ccttgccgcc gacccgctga ttaaacccgc acaaattgtt
      841 gatggtgcaa catggacgcg cacgatgggc tggaccgagt accagcaggt acgctacgcc
      901 accgcacgct cagtcttcaa atgggatggc accgataccg tcaaagtcgg cagcgatgaa
      961 accctggttc gcgtgctgga cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc
     1021 tactggatcg acagcgaagg gcaaattcgc cagtcggaac agtatctcgg cgcggattat
     1081 ttcccggtga aaaccactct catcaaggcg gcaaaacaat gattaaacaa acttttgtcg
     1141 cgttgctttt gagtgtggga gcgtcatcgg tcttcgcggc aggaaccgtc aaggtgttca
     1201 gcaatggcag cagtgaggcc aaaacactaa caggcgcaga gcatttaatc gatctggtag
     1261 gccaaccgcg gctggcaaac agctggtggc ccggtgcggt gattagcgaa gagctggcaa
     1321 cggcggcagc attgcgtcag cagcaggcgt tgctgacacg gctggcagaa cagggcgcag
     1381 attccagcac cgacgatgcc gccgccatta acgccttacg ccagcaaatc caggcattga
     1441 aagtgacagg caggcaaaaa atcaatcttg atcccgatat cgtccgcgtt gccgaacgcg
     1501 gtaacccgcc gttgcagggc aactacacgc tgtgggtcgg accaccgccg tccacggtca
     1561 cgttgttcgg gcttatcagc catcctggca atcagccatt cactccaggt cgcgacgtgg
     1621 cgagctatct ctccgggcag aacctgctta gcggtgcgga tcgcagctac gcgtgggtcg
     1681 tttacccgga cggacgcacg caaaaagcgc cggtggctta ctggaacaag cgtcacgtag
     1741 agccgatgcc cggcagcatt atttatgttg gcctcgcgga ctccgtctgg agtgagacgc
     1801 ctgatgccct taacgccgac attcttcaga ctctgacgca gcggataccc caataatgaa
     1861 aaaaagacat ctgcttagct tactggcgct gggcattagc atggcttgct acggcgaaac
     1921 atatcctgcg cccattggcc cgtcgcagtc ggatttcggt ggcgtaggat tattacaaac
     1981 tcccaccgcg cgtatggcac gggaagggga gttgagtctg aactatcgcg ataacgatca
     2041 gtaccgttat tactcagctt cagtgcaact cttcccgtgg ctggaaacaa cgctgcgcta
     2101 caccgacgtg cgcacccggc agtacagcag cgtcgaagcg ttctctggcg atcaaacgta
     2161 taaagataaa gccttcgatc tcaaactgcg tttgtgggaa gagagttatt ggctgccgca
     2221 agtggcggtt ggtgcgcggg atatcggcgg tacggggctg tttgatgcgg aatatcttgt
     2281 tgccagcaaa gcctgggggc cgttcgattt tacgctcggc ctgggctggg gatatctggg
     2341 caccagcggt aatgtgaaaa atccgctctg ttcagccagt gataaatatt gctatcgcga
     2401 taacagctac aaacaggcgg gatctatcga cggtagccag atgttccacg gtcctgcctc
     2461 actgtttggc ggcgtggaat accagacgcc ctggcaaccg ctgcgcctga aactggagta
     2521 tgaaggcaat aattatcagc aggattttgc cgggaagctg gagcaaaaaa gtaagtttaa
     2581 cgtcggtgcg atttatcgcg ttaccgattg ggccgacgtt aaccttagct atgaacgtgg
     2641 caacaccttt atgtttggcg ttacgttgcg caccaacttt aacgatctgc gcccgtctta
     2701 caacgataac gcccgcccgc aatatcaacc gcagccgcag gatgccattt tgcagcattc
     2761 ggtggtggcg aatcagttaa cgttgctgaa atacaatgcc ggacttgccg atccgcagat
     2821 ccaggcgaaa ggcgatacgc tgtatgttac cggcgagcag gtgaaatatc gtgattcgcg
     2881 cgaagggatc atccgtgcga atcggatcgt gatgaacgat ctgccggatg ggatcaaaac
     2941 gatccgcatt acagaaaatc gccttaacat gccgcaggtg acgacggaaa ccgatgtcgc
     3001 cagcttgaaa aatcatctcg ccggggagcc gctgggccat gaaacgaagg tggcgcaaaa
     3061 acgcgtcgag ccagtggttc cgaagtccac cgagcaggga tggtatatcg acaaatcacg
     3121 ctttgatttc catatcgatc cggtgctgaa ccagtcggtc ggtggcccgg aaaactttta
     3181 catgtatcag ctgggcgtga tgggaacggc agatttgtgg ctgacggacc atctgctgac
     3241 caccggcagc ctgtttgcaa atcttgccaa caactacgac aagtttaact acaccaatcc
     3301 gccgcaggac tcgcacttac cgcgcgttcg tacccatgtg cgcgagtatg tgcagaacga
     3361 tgtctatgtg aataacctgc aagccaacta cttccagcat ctgggcaacg gcttctacgg
     3421 tcaggtctat ggaggttatc tcgaaaccat gtttggcggt gccggggcag aagtgttgta
     3481 tcgtccgctg gacagcaact gggcgtttgg tctggatgcc aactacgtta aacagcgcga
     3541 ctggcgtagt gcaaaagata tgatgaaatt caccgactac agcgtgaaaa ccggacatct
     3601 gaccgcctac tggacgccat ctttcgccca ggacgtgttg gtgaaagcga gcgtcgggca
     3661 gtatctggca ggggataaag gcggcacgct ggagatcgcc aaacgctttg atagcggcgt
     3721 ggtggttggc ggctatgcca cgatcacgaa tgtttcgaaa gaggagtacg gcgaagggga
     3781 cttcaccaaa ggcgtgtatg tctctgtgcc gttggatctc ttctcgtctg gcccgacacg
     3841 cagccgtgcg gcgattggct ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg
     3901 taagttccag ttgtatgaca tgaccagcga ccgtagcgtc aatttccgct aagtcatggg
     3961 aaaggtgcca gttttcgcaa tcactggcac cctcattctt aatggcctgt tgagccatca
     4021 ggaacgcagt tcacccccgg tttgcgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc
     4081 ctgtccgtcg atatcggttg cagatctggt tgcctgagcg actgggccat cattgggctg
     4141 gagacattgc tttgcggtag gtcggcttta tcagcagtac cgagcggacc agcataagca
     4201 ggaagaacag agactgataa catcaaagca gcaaaataag gcttcatttt taccaccttt
     4261 atcaggttac gtttcatttg ttccagagga acattgtcga tttttcgcgc attgctggtg
     4321 gctgggaatc acctgaatgg gtgatttttg aattaccggc tttggtgcgg tttgtcttgc
     4381 cggatgcgcc gccaggcgcg gcttatccgg cctacgggta ggtatatccg gctttggtgg
     4441 aggcgcgctc caaatccagg ttgaacaaaa catacacaaa aaatatagat ctccgtcaca
     4501 tttttgcgtt atacaggaag ctcgccactg tgaaggaggt actgctatga cgtcactctc
     4561 tcgtccgcgc gtggagttta tctccaccat tttgcagacc gtactcaatc ttggcctgtt
     4621 gtgcctcggc ctgattttgg ttgtcttcct cggcaaagaa acggtgcatc tggctgatgt
     4681 gctgttcgcg ccagaacaaa ccagcaaata tgagctggta gaagggctgg tggtttactt
     4741 tctctatttc gaatttatcg cgctgattgt gaagtacttt cagtccggtt ttcacttccc
     4801 gctacgttac tttgtctata ttgggatcac cgccattgtg cgcttgatca tcgtcgatca
     4861 taaatcgcca ctggatgtat tgatctactc ggcagcgatc ctgttactgg tgatcaccct
     4921 gtggctgtgt aattcgaaac ggctgaagcg ggagtaaaaa gacagcacgc cgaactggcg
     4981 cggcgtgctg gacaggaaga ttacagtgta gcagtttgtt gtgttttctt cgtttccggt
     5041 tcccagaggg cttccagctc ctcaagggtt ttacctttgg tttccgggac aaatttccac
     5101 ataaatagtg ctgccagaac gcccatacaa ccgtaaatcc agtaggagaa accgttgtgg
     5161 aaatgggtca ccagccagga gtttttgtcc atcatcggga aggtccagga gacgaagtag
     5221 ttcgccagcc actgggccgc caccgcgatt gccagcgctt taccacgaat agcattcggg
     5281 aagatttccg acagcagtac ccagcatacc ggaccccagg acatggcaaa ggcagcaaca
     5341 tagaacagca tcgacagtag cgccacaata cccggtgcct gagtgtaaaa cgcggtaccg
     5401 aggctaaaca taccgattgc cattccgagc gcgccgataa tttgcagtgg cttacgacca
     5461 aatttatcca ccgtcataat tgccagaacg gtgaaggtga ggttgataac tccgacaata
     5521 atggtctgca acagcgcgat atccgtgctg gcccccagcg ttttgaacac ttccggcgcg
     5581 tagtacaaca ccacattgat gccgacaaat tgctggaaga tggagagcat aacgccgata
     5641 acaatcacgc ccacgccgaa catcagcaga cgaccaccgg ttttgcgtcc atgatccagg
     5701 gagtgtttaa tttcctgcac cgcctgggct gccaaagcgt tgcccataat tttgcgcagg
     5761 atgccttccg cttgttcttg cttaccgcgt gacatcagcc agcgagggct ttccggcacg
     5821 gtataaagca gcattaagaa cagcagcgca gggatacatt ccgaagcaaa catataacgc
     5881 cagccgtcag tattcagcca ggtggcatcg ccggaacggg caataaaata gtttacgcag
     5941 taaactaaaa gttggccgaa aataatcgca aactggttaa aagagaccag cttcccacga
     6001 atatgtgcag gagccagttc cgcaatatac attggtgaga gcattgaggc taaaccaaca
     6061 ccaataccgc caataatgcg ataaataaca aattccggga cataacctgc cagataaaca
     6121 ggcactgtgt tgtccgggtt tatagaggta aaaccaagtt ctggccaggc agaacctaca
     6181 ccagaaataa aaaacaggac agcagcaatc ttaagtgaat cacgacgacc gaagcggtta
     6241 ctgcaataac caccgagggc accgccgatg atgcaaccaa tcagagcgct ggctacgcaa
     6301 aaccctaaca gggagttggc agcggattca cttaagtttt gtggagcaac aaagacggta
     6361 tggagtgact caacagtacc ggaaataacg gcggtgtcgt agccaaataa taaaccacct
     6421 aatgtagcga ctaaggtaat cgaaaatata taactggaat tatactgggt attcattcag
     6481 acctgcctta gaccattctg atcttttatc agtgcattga tgttggaatt gtcaggcagt
     6541 taaggcaatt atcatttttt acaatgcgtt tacgtaatgt cttttctgtg atcttaattg
     6601 tgataattat ctaaaattga tgaaaacaat actattgccg tgactcagag cacgaaagag
     6661 aattatcgta agtgggaaaa caaataacgt aaaaataata agctctatga tgggaatatt
     6721 aaccggcgaa cgattcagat tgcagacgaa agaaaaaaag gcgctccgtg gagcgccgaa
     6781 taacagtcac aagttgggat aacgtaagtt gagggtgcag cggcataaca ttggcagaac
     6841 aacatcttta acctttcaca ccacctgccg tcaggccgtt gaccagccag cgttgagcca
     6901 gcaagaagac gatggtgatc ggtaatgcag acatcacggc agcggcggca aagtcacccc
     6961 acaggtagtt ttgcgggttg aggtattgct gcatccccac ggccagggtg tagctgttta
     7021 cgtcacgcag taacagcgac gcgaccggaa cttcagtaat ggcagcgata aacgacaaga
     7081 tgaataccac cgccagaatc ggtactgaca gcggcaacag gacaaggcgg aaggcctgcc
     7141 acggtgtcgc accatccagc gcagccgctt cttccagcga actatcgatg gtttcgaaat
     7201 agcctttgat ggtccagaca tgcagcgcaa tcccacccag atacgcgaaa attacgccgc
     7261 cgtgagtatt caggccaatg aatggaatgt actcacccag acgatcaaac aacgcataca
     7321 acgcgaccag tgaaagtact gccgggaaca tctggaaaat cagcattcct ttcagcagcg
     7381 tcgctttgcc tggaaagcgc atacgggcga aagcgtaggc gcaggtggta gagagcgcca
     7441 caatgccaat cgcggaaatc ccggcgacct ttaccgagtt ccacagccac agcagtaccg
     7501 ggaatggcgg tggcgtaatg cgaccatcag cctgttcaac gctaaaacct aacgccagtt
     7561 tccagtgatc ccaggagatt tgctccggga tcaggctacc ggtcgcgaag tttccctgac
     7621 gcagcgagat agcgacgacc atcagcagcg ggaacataat cgccgcgata aaaagtagca
     7681 gtagcaggtg agtaataaac aaacgtgctt tttgcgattt cggttggacc attgccattt
     7741 ttgttatctc ccttaatcaa acttcattcg cgtggctttc aggttcacta tcgccagcgc
     7801 acccaccagc aggaagatca gcgtggcaat tgctgctgcc agaccgaagt cctgaccccc
     7861 gccgccttca aaagcgatgc ggtaggtgta gttaacaagc aggtcggtat aaccggctgg
     7921 cgtggtcgtg ccaagacgat ccgggccgcc gttggttaac agttgaatca gcacgaagtt
     7981 gttaaagtta aaggcgaagc tggcgatcat cagcggcgtc agcggtttaa tcagcagcgg
     8041 cagcgtaatc ttaaagaagt tctggaacgg acctgcgcca tccattgctg aggcttcata
     8101 caaatcgtcc ggaatcgctt tcagcaagcc catgcagagg atcatcatgt acggataacc
     8161 cagccaggta ttgacgataa tcagcatcgt gcgggcggtg gtcggatcgc tgaaccaggc
     8221 gggcttcacg ccaaacagcg cgctcaacat catgttgatt tcaccgaagc tctggttaaa
     8281 caaccctttg aaaatcaaga ttgaaatgaa cgatggcacc gcgtagggca gaatcagcag
     8341 gacgcgatag accgctttgc cgcgcaacgc ttcccactgc accagacacg ccagaaccat
     8401 gccgaccgcc accgttaaaa agacagtgat cagcgagaac accacggtcc agacgaaaat
     8461 ggcgaggaac ggtttctgaa tgccttcgtc ggtgaagacg cgggtaaagt ttttccagcc
     8521 agtagtcacg gtgtaaccgg ggcttagctt ttcatcgccc cagttgccgt cggcggtaat
     8581 ggactggtaa aagccaattt ggttattcgg acgatatttc acgccgctct gattattcgt
     8641 caacgtgccg tcaccgtcga gtgtgtagag cggctgcgtg ccagaaaact ggcgcaggga
     8701 gctcatcatc actttgttgc catccggcag aatggcggta atgtcactca gcgcctgacg
     8761 attctgggta atcacgcgca gattcgcgcg ttcgccttcg ggctgggtgg tcgtttcttt
     8821 cagttgcagt ttttgctcgc cgccaaattt aaaagcgtcg gagaggtaat ttttgccggt
     8881 ttcgccgtcg ctgagggcta gttgccactc atcgcccgcc gggtaaagac caaaattata
     8941 ggttttgcct gcttgccagg agcgatctag caacacttcc tgcgcacgtt caaaagtcag
     9001 ctggttagtg ctgctgtagt tggtgaaggc aatggcgatg gtgcagacca gagggaagag
     9061 gacgaataat cccattccag ccattcccgg gtaaacatag cgccaggcgt aggctttacg
     9121 attggcgaaa atatacagcc ccgctgaact caatatcagc gtggtaatgg cgaacaggta
     9181 ttccccttgt gcgtacatta aaacaacaag gtaacccacc agcaggccga gcagacctaa
     9241 cactgaccat ttcagcgcgt cgctttgcca ccaatgtttc tttttaatga catccatggg
     9301 gttcttcctc attccaggac ggataaggct ttcacgcctt atccgacaac aactgcctga
     9361 tgcgacgctg acgcgtctta tcaggcctac atacgtttcg gttttgtagg ccggacaagg
     9421 cgttcacgcc gcatccggca tttcacagca ttacttggtg atacgagtct gcgcgtcttt
     9481 cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag tacgcacggc
     9541 ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct gggcgttttc
     9601 catggtggct gcaatacgtg gatctttcgc caactcttcc tcgtaagact tcagcgctac
     9661 ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcgtcagtca gcagatagtt
     9721 ttcgaggaac tctttcgcca gctctttgtt cggactggcg gcgttaatac cggcgctcag
     9781 cacgccaacg aacggtttag atggttgacc cttgaaggtc ggcagtaccg ttacaccata
     9841 attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg tcatcgctgt
     9901 ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt
     9961 gtttttaatc aggtcaacca ggaaggtcag acccgctttc gcgccagcgt tatccacgcc
    10021 cacgtcttta atgtcgtatt tgccattttc atacttgaac gcataacccc cgtcagcagc
    10081 aatcagcggc caggtgaagt acggttcttg caggttgaac atcagcgcgc tcttaccttt
    10141 cgctttcagt tctttatcca gcgccgggat ctcttcccag gtttttggtg ggttcggcag
    10201 cagatctttg ttataaatca gcgataacgc ttcaacagcg atcgggtaag caatcagctt
    10261 gccgttgtaa cgtacggcat cccaggtaaa cggatacagc ttgtcctgga acgctttgtc
    10321 cggggtgatt tcagccaaca ggccagattg agcgtagcca ccaaaacggt cgtgcgccca
    10381 gaagataatg tcagggccat cgccagttgc cgcaacctga gggaatttct cttccagttt
    10441 atccggatgc tcaacggtga ctttaattcc ggtatctttc tcgaatttct taccgacttc
    10501 agcgagaccg ttatagcctt tatcgccgtt aatccagatt accagtttac cttcttcgat
    10561 tttggcgaga gccgaggcgg aaaacatcat cgtcgttaat gcggataatg cgaggatgcg
    10621 tgcacctgtt tttattttca taatctatgg tccttgttgg tgaagtgctc gtgaaaacac
    10681 ctaaacggac tctagtttct ttatacggca acctctttcc atcctccttg cccctacgcc
    10741 ccaccgttgc tttgtgtgat ctctgttaca gaattggcgg taatgtggag atgcgcacat
    10801 aaaatcgcca cgatttttgc aagcaacatc acgaaattcc ttacatgacc tcggtttagt
    10861 tcacagaagc cgtgttctca tcctcccgcc tcctccccca taaaaaagcc agggggtgga
    10921 ggatttaagc catctcctga tgacgcatag tcagcccatc atgaatgttg ctgtcgatga
    10981 caggttgtta caaagggaga agggcatggc gagcgtacag ctgcaaaatg taacgaaagc
    11041 ctggggcgag gtcgtggtat cgaaagatat caatctcgat atccatgaag gtgaattcgt
    11101 ggtgtttgtc ggaccgtctg gctgcggtaa atcgacttta ctgcgcatga ttgccgggct
    11161 tgagacgatc accagcggcg acctgttcat cggtgagaaa cggatgaatg acactccgcc
    11221 agcagaacgc ggcgttggta tggtgtttca gtcttacgcg ctctatcccc acctgtcagt
    11281 agcagaaaac atgtcatttg gcctgaaact ggctggcgca aaaaaagagg tgattaacca
    11341 acgcgttaac caggtggcgg aagtgctgca actggcgcat ttgctggatc gcaaaccgaa
    11401 agcgctctct ggcggtcagc gtcagcgtgt ggcgattggc cgtacgctgg tggccgagcc
    11461 aagcgtattt ttgctcgatg aaccgctctc caacctcgat gctgcactgc gtgtgcaaat
    11521 gcgtatcgaa atctcccgtc tgcataaacg cctgggccgc acaatgattt acgtcaccca
    11581 cgatcaggtc gaagcgatga cgctggccga caaaatcgtg gtgctggacg caggtcgcgt
    11641 ggcgcaggtt gggaaaccgc tggagctgta ccactatccg gcagaccgtt ttgtcgccgg
    11701 atttatcggt tcgccaaaga tgaacttcct gccggtaaaa gtgaccgcca ccgcaatcga
    11761 tcaagtgcag gtggagctgc cgatgccaaa tcgtcagcaa gtctggctgc cagttgaaag
    11821 ccgtgatgtc caggttggag ccaatatgtc gctgggtatt cgcccggaac atctactgcc
    11881 gagtgatatc gctgacgtca tccttgaggg tgaagttcag gtcgtcgagc aactcggcaa
    11941 cgaaacccaa atccatatcc agatcccttc cattcgtcaa aacctggtgt accgccagaa
    12001 cgacgtggtg ttggtagaag aaggtgccac attcgctatc ggcctgccgc cagagcgttg
    12061 ccatctgttc cgtgaggatg gcactgcatg tcgtcgactg cataaggagc cgggcgttta
    12121 agcaccccac aaaacacaca aagcctgtca caggtgatgt gaaaaaagaa aagcaatgac
    12181 tcaggagata gaatgatgat tactctgcgc aaacttcctc tggcggttgc cgtcgcagcg
    12241 ggcgtaatgt ctgctcaggc aatggctgtt gatttccacg gctatgcacg ttccggtatt
    12301 ggctggacag gtagcggcgg tgaacaacag tgtttccaga ctaccggtgc tcaaagtaaa
    12361 taccgtcttg gcaacgaatg tgaaacttat gctgaattaa aattgggtca ggaagtgtgg
    12421 aaagagggcg ataagagctt ctatttcgac actaacgtgg cctattccgt cgcgcaacag
    12481 aatgactggg aagctaccga tccggccttc cgtgaagcaa acgtgcaggg taaaaacctg
    12541 atcgaatggc tgccaggttc caccatctgg gcaggtaagc gcttctacca acgtcatgac
    12601 gttcatatga tcgacttcta ctactgggat atttctggtc ctggtgccgg tctggaaaac
    12661 atcgatgttg gcttcggtaa actctctctg gcagcaaccc gctcctctga agctggtggt
    12721 tcttcctctt ttgccagcaa caatatttat gactatacca acgaaaccgc gaacgacgtt
    12781 ttcgatgtgc gtttagcgca gatggaaatc aacccgggcg gcacattaga actgggtgtc
    12841 gactacggtc gtgccaacct gcgtgataac tatcgtctgg ttgatggcgc atcgaaagac
    12901 ggctggttat tcactgctga acatactcag agtgtcctga agggctttaa caagtttgtt
    12961 gttcagtacg ctactgactc gatgacctcg cagggtaaag gtctgtcgca gggttctggc
    13021 gtcgcgtttg ataacgaaaa atttgcctac aatatcaaca acaacggtca catgctgcgt
    13081 atcctcgacc acggtgcgat ctccatgggc gacaactggg acatgatgta cgtgggtatg
    13141 taccaggata tcaactggga taacgacaac ggcaccaagt ggtggaccgt tggtattcgc
    13201 ccgatgtaca agtggacgcc aatcatgagc accgtgatgg aaatcggcta cgacaacgtc
    13261 gaatcccagc gcaccggcga caagaacaat cagtacaaaa ttaccctcgc acaacaatgg
    13321 caggctggcg acagcatctg gtcacgcccg gctattcgtg tcttcgcaac ctacgccaag
    13381 tgggatgaga aatggggtta tgactacaac ggcgatagca aggttaaccc gaactacggc
    13441 aaagccgttc ctgctgattt caacggcggc agcttcggtc gtggcgacag cgacgagtgg
    13501 accttcggtg cccagatgga aatctggtgg taatagcaaa acctgggccg gataaggcgt
    13561 ttacgccgca ttcggcaacc aacgcctgat gcgacgcttg cgcgtcttat caggccgaca
    13621 acggctgtca aatgtaggcc ggataaggcg tttacgccgc atccggcata aaaacaggtt
    13681 gtcattatct gaaaggggcg aaagcccctc tgattatcgg gtttagcgcg ctattgcctg
    13741 gctaccgctg agctccagat tttgaggtga aaacaatgaa aatgaataaa agtctcatcg
    13801 ccctctgttt atcagcaggg ttactggcaa gcgcgcctgg aatcagcctt gccgatgtta
    13861 actacgtacc gcaaaacacc agcgacgcgc cagccattcc atctgctgcg ctgcaacaac
    13921 tcacctggac accggtcgat caatctaaaa cccagaccac ccaactggcg accggcggcc
    13981 aacaactgaa cgttcccggc atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca
    14041 ttggcgaact gaccctgacg ctgaccagcg aagtgaacaa acaaaccagc gtttttgcgc
    14101 cgaacgtgct gattcttgat cagaacatga ccccatcagc cttcttcccc agcagttatt
    14161 tcacctacca ggaaccaggc gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga
    14221 caccggcgtt ggggcagcaa aaactttatg ttctggtctt taccacggaa aaagatctcc
    14281 agcagacgac ccaactgctc gacccggcta aagcctatgc caagggcgtc ggtaactcga
    14341 tcccggatat ccccgatccg gttgctcgtc ataccaccga tggcttactg aaactgaaag
    14401 tgaaaacgaa ctccagctcc agcgtgttgg taggaccttt atttggttct tccgctccag
    14461 ctccggttac ggtaggtaac acggcggcac cagctgtggc tgcacccgct ccggcaccgg
    14521 tgaagaaaag cgagccgatg ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa
    14581 acgctgtcgc gaaaggtgat gttgataagg cgttaaaact gcttgatgaa gctgaacgcc
    14641 tgggatcgac atctgcccgt tccaccttta tcagcagtgt aaaaggcaag gggtaattac
    14701 gccccacagt gctgattttg caacaactgg tgcgtctcct ggcgcacctt tttttatgct
    14761 tccttcctgg gatatgagcg attttttata gtaactcact tcttcttcac taagaatatc
    14821 cattatctca atgccttatc agagattctt ttcctttcgc cggtagtgtc tggacattca
    14881 ggctactttt ccaggttatt ttatttctgt tatgcagagg ttttatgata agtcatatcc
    14941 taaattctgg cggcaataac tctttgatga aacatgatgt ggtgcaagga aataatatag
    15001 tagatcttga tttactacgt aatttcaatg gggtgccagg tttaaac
//
LOCUS       DAZFKM010000124        14981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFKM010000124 DAZFKM010000000
VERSION     DAZFKM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            113..631
                     /gene="yfiR"
                     /locus_tag="ACHKAY_003900"
     CDS             113..631
                     /gene="yfiR"
                     /locus_tag="ACHKAY_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO5702492.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            621..1847
                     /gene="dgcN"
                     /locus_tag="ACHKAY_003901"
     CDS             621..1847
                     /gene="dgcN"
                     /locus_tag="ACHKAY_003901"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO5702493.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1863..2345
                     /gene="yfiB"
                     /locus_tag="ACHKAY_003902"
     CDS             1863..2345
                     /gene="yfiB"
                     /locus_tag="ACHKAY_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO5702494.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2422..2769)
                     /gene="rplS"
                     /locus_tag="ACHKAY_003903"
     CDS             complement(2422..2769)
                     /gene="rplS"
                     /locus_tag="ACHKAY_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO5702495.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2811..3578)
                     /gene="trmD"
                     /locus_tag="ACHKAY_003904"
     CDS             complement(2811..3578)
                     /gene="trmD"
                     /locus_tag="ACHKAY_003904"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO5702496.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3609..4157)
                     /gene="rimM"
                     /locus_tag="ACHKAY_003905"
     CDS             complement(3609..4157)
                     /gene="rimM"
                     /locus_tag="ACHKAY_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO5702497.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4176..4424)
                     /gene="rpsP"
                     /locus_tag="ACHKAY_003906"
     CDS             complement(4176..4424)
                     /gene="rpsP"
                     /locus_tag="ACHKAY_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO5702498.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4673..6034)
                     /gene="ffh"
                     /locus_tag="ACHKAY_003907"
     CDS             complement(4673..6034)
                     /gene="ffh"
                     /locus_tag="ACHKAY_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO5702499.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6201..6992
                     /gene="ypjD"
                     /locus_tag="ACHKAY_003908"
     CDS             6201..6992
                     /gene="ypjD"
                     /locus_tag="ACHKAY_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO5702500.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            7013..8299
                     /gene="yfjD"
                     /locus_tag="ACHKAY_003909"
     CDS             7013..8299
                     /gene="yfjD"
                     /locus_tag="ACHKAY_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO5702501.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8354..8947)
                     /gene="grpE"
                     /locus_tag="ACHKAY_003910"
     CDS             complement(8354..8947)
                     /gene="grpE"
                     /locus_tag="ACHKAY_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO5702502.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9070..9948
                     /gene="nadK"
                     /locus_tag="ACHKAY_003911"
     CDS             9070..9948
                     /gene="nadK"
                     /locus_tag="ACHKAY_003911"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO5702503.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10034..11695
                     /gene="recN"
                     /locus_tag="ACHKAY_003912"
     CDS             10034..11695
                     /gene="recN"
                     /locus_tag="ACHKAY_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO5702504.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11844..12185
                     /gene="bamE"
                     /locus_tag="ACHKAY_003913"
     CDS             11844..12185
                     /gene="bamE"
                     /locus_tag="ACHKAY_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO5702505.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12247..12537)
                     /gene="yfjF"
                     /locus_tag="ACHKAY_003914"
     CDS             complement(12247..12537)
                     /gene="yfjF"
                     /locus_tag="ACHKAY_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO5702506.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12527..12964)
                     /gene="ratA"
                     /locus_tag="ACHKAY_003915"
     CDS             complement(12527..12964)
                     /gene="ratA"
                     /locus_tag="ACHKAY_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO5702507.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13135..13617
                     /gene="smpB"
                     /locus_tag="ACHKAY_003916"
     CDS             13135..13617
                     /gene="smpB"
                     /locus_tag="ACHKAY_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO5702508.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13832..14194
                     /gene="ssrA"
                     /locus_tag="ACHKAY_003917"
     tmRNA           13832..14194
                     /gene="ssrA"
                     /locus_tag="ACHKAY_003917"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14465..14713
                     /locus_tag="ACHKAY_003918"
                     /pseudo
     CDS             14465..14713
                     /locus_tag="ACHKAY_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
BASE COUNT         3804 a         3948 c         3515 g         3714 t
ORIGIN      
        1 tcattgtgtt ttatttctca cattgatgac gatcgcatga aaaaatgata ttttaatgag
       61 cattctgaaa aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt
      121 ttctcaccga ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca
      181 ggagccttct gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac
      241 ccgctggcct gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag
      301 taccgcccta caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac
      361 tcaacaagag gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac
      421 attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca
      481 aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag
      541 atttgccgta aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt
      601 aatgctcgca cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt
      661 ttaaaagagc attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct
      721 ggttgctgct ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg
      781 cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc
      841 ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg gcaattttca actgcagaag
      901 tacgtgataa gcagcaaaat attctggcgt cctggcatta cacccgtaag gatccaggcg
      961 atactttcag caatttcata agccactggc tcttccccgc ccccatcatt cagccgattc
     1021 gtcacaatgg tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc
     1081 attttatctg gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg
     1141 caattaccct cacccgccat ttgcacaatg gcctggtaga agcgctgaaa aatatcaccg
     1201 atgtcgtaca tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta
     1261 tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt
     1321 ggcagcttcg tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat
     1381 taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt
     1441 ccgatgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca
     1501 atgatacctg gggtcatgcg acgggcgata gagtcttgat tgaaatcgca aaacggttag
     1561 ctgaatttgg cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg
     1621 tgctctatga tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa
     1681 tctttaatct cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg
     1741 gttacgcgat gaccattgag cacgcctctg cgaaaaaatt acaagagctt gccgatcaca
     1801 atatgtatca ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg
     1861 atatgataaa gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc
     1921 agtcccctca gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta
     1981 ctgaatccgc cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg
     2041 actacaaatt gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct
     2101 cgacagggct aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt
     2161 acaacgaagg cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctatgggtg
     2221 gacaaattcc gcgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca
     2281 gtaacaagac cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attactaccc
     2341 cttaaaacac cagcaaacag ataaaaaaag ggccagccaa ttggccagcc cttcttaaca
     2401 ggatgtcgct taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt
     2461 acgctcacgc aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat
     2521 gctgtcaact accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat
     2581 tttacgaaca gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc
     2641 gaatgcctgc agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc
     2701 acccggacgg aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat
     2761 gttgctcata atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc
     2821 ccatcatgtt tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct
     2881 tgctcttcag tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc
     2941 agcgactgtt tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc
     3001 ggaacttcca tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct
     3061 tcagcaaagg aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg
     3121 gaaacggagt caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg
     3181 attgaccatt cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga
     3241 ccgcacacca gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc
     3301 ttgcgtccct gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct
     3361 gcatgaatgg cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa
     3421 ggacgatcgt ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg
     3481 atgctcagca ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg
     3541 aacatttcag gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat
     3601 ccggtggttt aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact
     3661 ttcttgataa cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct
     3721 ttcaggtttg ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact
     3781 ttaccgagat cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag
     3841 tagtccccct cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc
     3901 agcaggttcg ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg
     3961 tggtgcttcc agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag
     4021 ggctgatagt caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca
     4081 cgaataccgt aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg
     4141 gtgagttgtt tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg
     4201 atcagcgcag caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga
     4261 tccagatcca ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg
     4321 cgctcgatga agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac
     4381 ggacgctttt tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg
     4441 aataaaacaa ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa
     4501 ttttactcat ttttgcggga attgcaatca acagttgcta actctgctgt aaaaggccgt
     4561 cggcggtgca gccagtttgg tgccggagtg cgcgcagtca ccggagcgta cacgcagtac
     4621 gtgaggatga cgagcacatc ccggtgccaa aatggcaaac aagccaggcc gattagcgac
     4681 cagggaagcc tgggggcatc atacccttca tgcttctcat catcttcgcc attccgcctt
     4741 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttcacgt
     4801 cctgcacctg cataccgcaa ccggcagcga tacgacgctt acgcgaacct ttgatgattt
     4861 ctggcttagc gcgctctttt atcgtcatcg agttgatgat ggcttccata cgcaccagca
     4921 ctttatcgtc catctgcgac ttaacattat ccgggatctg ccccatgccc ggcagcttgc
     4981 ccatcaggct agccatgccg cccatatttt tcatctggcg cagttgctca agaaaatcgt
     5041 tgagatcgaa gccgtcgcct tttttcagct tgctggctaa tttctctgcc tgcgcgcggt
     5101 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     5161 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagaa
     5221 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
     5281 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
     5341 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
     5401 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
     5461 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
     5521 cgatatctac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
     5581 ccagcgtctc aagctgtttg attgccgccg ggcgataaac gtcggcagaa accaccagca
     5641 ctttcttctt gtgcttctcg cgcaggaact taccgagttt accaacgctg gttgttttac
     5701 cggcaccttg caggcccgcc atcagtacga ccgcaggcgg ttgcgcagcc aggttcaggg
     5761 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gactattttg acgaactcct
     5821 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
     5881 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
     5941 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
     6001 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
     6061 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
     6121 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
     6181 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
     6241 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
     6301 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
     6361 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
     6421 tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
     6481 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
     6541 acatcaccca tctggaagca acgcctggga tgctggtgca cattggctta tcgctctttt
     6601 cctatgccac gctgataatt gccgccctgt acgcgctaca actggcgtgg attgattacc
     6661 aactgaagaa caagaagctg gcgtttaacc aagaaatgcc gccattgatg agtatcgagc
     6721 gtaaaatgtt ccacatcacg cagattggcg tggtgctact aacgctcacg ctttgcactg
     6781 gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggcc gtgctctcta
     6841 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttatcatgaa ggatggcgtg
     6901 gacgccgcgt cgtctggttt aacgttgcgg gtgcagtcat cctgacactg gcctacttcg
     6961 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
     7021 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
     7081 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtctgcgac atatggcgaa
     7141 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
     7201 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
     7261 tgttgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
     7321 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
     7381 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
     7441 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
     7501 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcacg aatcacgctc
     7561 acaaatttcc cgtcgcaatc aggatatgct actgtctgtg ctcgatctgg aaaagatgac
     7621 cgttgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca acgatgactg
     7681 gaaatcgatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tctaccgtga
     7741 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
     7801 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
     7861 ggaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
     7921 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
     7981 agagattgtc ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
     8041 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
     8101 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
     8161 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcgagtacg atatagatat
     8221 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
     8281 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
     8341 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
     8401 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
     8461 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
     8521 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
     8581 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
     8641 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
     8701 ctttttcaat atccagttca gtacgtcgac gcaggttttc catttcggct tttacacgca
     8761 aaatgccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
     8821 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
     8881 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
     8941 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
     9001 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
     9061 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcacccc
     9121 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
     9181 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
     9241 gcggagattg ggcaactagc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
     9301 ggcgcggcac gcacactcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
     9361 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacagttagc cgatgtgctg
     9421 gaaggccact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
     9481 tgccagaaac gcatcagcac cgcgataaat gaagtggtgc ttcatccagg caaagtggcg
     9541 catatgattg agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
     9601 ggactaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc aggcggtcct
     9661 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
     9721 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
     9781 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
     9841 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
     9901 ttcaacacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
     9961 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    10021 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    10081 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggcg cgggtaaatc
    10141 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    10201 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    10261 tctgcgctgg ctggaagaaa atcagcttga agacgggcat gaatgtttgc ttcgtcgagt
    10321 gatcagcagc gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    10381 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    10441 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    10501 gcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    10561 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    10621 acttaacgaa tttaatccgc agcccggaga gtttgaacaa atcgacgaag agtacaaacg
    10681 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    10741 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    10801 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    10861 gattgctgaa gccagcgatg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    10921 ccgactattt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    10981 cgtcagccct gaggcattgc cacagtatta ccagtcgcta ctggaagaac agcagcaact
    11041 ggacgatcag gccgactcac aagaaacgct tgcgctggcg gtaacgaaac atcatcagca
    11101 ggcactggaa atcgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    11161 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgatcga
    11221 tgttaaattt gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    11281 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    11341 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    11401 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    11461 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    11521 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    11581 gcatatgcaa tccctgaata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    11641 cagtgaagtc acacgtaata cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    11701 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    11761 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    11821 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    11881 tattgatgtt gaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    11941 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    12001 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    12061 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    12121 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    12181 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    12241 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    12301 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    12361 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    12421 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcacgcgctg
    12481 caggtactgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctggcactgt
    12541 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agacgccagc tctttaaaca
    12601 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    12661 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    12721 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    12781 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    12841 actccagaat ccgacttccg gtacaacccg gcaaaaactg aggataagac tgaacgtcat
    12901 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    12961 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    13021 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    13081 caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac gcttatgacg
    13141 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    13201 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaggttaaa
    13261 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    13321 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    13381 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    13441 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    13501 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    13561 aaagaacgcg aatggcaggt ggataaagca cgtatcatga aaaacgccca ccgttaaacc
    13621 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    13681 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    13741 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    13801 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    13861 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    13921 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    13981 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    14041 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    14101 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    14161 tcggacgcgg gttcaactcc cgccagctcc accacttttg ataggactgc aatcggacag
    14221 cggcaataaa aacagccact tacggactct gaccagacag agcgcagacc gagaaaagac
    14281 aaaaaaatgc acgtaaaatg cacgtgcact ttaaaagaac cccagatctc acggtctggt
    14341 ttttttctgt ttgtaactaa tggtaacaaa aacacatcac ctcttggcgt tccttctcct
    14401 tgatactgtt tatccataca gttaaaaata acactgtata caaacacagt atagagagat
    14461 ttttatgcgt attgaaattt gcatagccaa agaaaaaatc agcaaaatgc caaaaggatc
    14521 tgttcctgcc ctacagcaag aaatgctgtg acgtgccagt aaacgttatg acgatgtaga
    14581 agtgatcatc aaatccacca gcaacgatgg cctttcagtt actctcaccg ccgataaaga
    14641 ttctgcaaaa acttttgttc aggagacgct gaaagatacc tgggagtctg ctgacgagtg
    14701 gtttgttcgc taattaacga gtaaaatcag taacggctgg aaatcattca atactcacac
    14761 tatctaaaag ttcaccagcc aacagcggta agttctttca tacgacgtgc cgcggatcct
    14821 attattctag tatgaaatcc ctaaccgatt tcaaattctc cagactgctc agattctctt
    14881 ttatcccacg ctggcgctga taaatctagt cattgcaatc gacctgcgct atcgctgccg
    14941 cagttcttcc agtttctgca tcgataactc catcttctga a
//
LOCUS       DAZFKM010000125        14810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFKM010000125 DAZFKM010000000
VERSION     DAZFKM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHKAY_003919"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHKAY_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5702509.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVG"
     gene            46..315
                     /locus_tag="ACHKAY_003920"
     CDS             46..315
                     /locus_tag="ACHKAY_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702510.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            519..1637
                     /gene="ybdK"
                     /locus_tag="ACHKAY_003921"
     CDS             519..1637
                     /gene="ybdK"
                     /locus_tag="ACHKAY_003921"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO5702511.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHKAY_003922"
     CDS             1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHKAY_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO5702512.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHKAY_003923"
     CDS             2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHKAY_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO5702513.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHKAY_003924"
     CDS             2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHKAY_003924"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO5702514.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHKAY_003925"
     CDS             3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHKAY_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO5702515.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHKAY_003926"
     CDS             complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHKAY_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO5702516.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHKAY_003927"
     CDS             complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHKAY_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO5702517.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHKAY_003928"
     CDS             complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHKAY_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO5702518.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHKAY_003929"
     CDS             complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHKAY_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO5702519.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHKAY_003930"
     CDS             complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHKAY_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO5702520.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            12446..13129
                     /gene="cusR"
                     /locus_tag="ACHKAY_003931"
     CDS             12446..13129
                     /gene="cusR"
                     /locus_tag="ACHKAY_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO5702521.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13119..14567
                     /gene="cusS"
                     /locus_tag="ACHKAY_003932"
     CDS             13119..14567
                     /gene="cusS"
                     /locus_tag="ACHKAY_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO5702522.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3578 a         3979 c         3661 g         3592 t
ORIGIN      
        1 tccccgacca gaagcgtaaa tcccagcacc gttaaacaca gaactatgac tgccgcaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt
      121 gttcaggttt gggtaggtag ccccagggtt gatagaaata tcgtcctggg acttcatctt
      181 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc
      241 ttgcttagca aaatagctgt ttatttatac agtaagtcct gtttcggact tctcactccc
      301 catttgatca actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt
      361 cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa ttgcttcagt
      421 caccatcacc aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctccgact
      481 tctatactga atagaaaacg ccaacttaag agaaacctat gccattaccc gattttcatg
      541 tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
      601 atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaatgag atcacggccg
      661 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
      721 atatcaacca ggctgccggg cagttttcag cgatgcagaa agtcgtattg caggcagccg
      781 cagaccatca tctggaaatt tgcggcggtg gcacgcaccc gtttcagaaa tggcagcgtc
      841 aggaggtatg cgacaacgaa cgctatcaac gaacgctgga aaactttggc tatctcatcc
      901 agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc gatgacgcca
      961 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
     1021 cgccgtatat gcagggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
     1081 cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
     1141 ttcgctgcct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
     1201 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
     1261 gccacgcggt aaatatggcg ggattaattc aggccaccgc ccactggtta ctgacggaac
     1321 gcccgtttaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
     1381 gccgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
     1441 cggaagatac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa atgggtgcat
     1501 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
     1561 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
     1621 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
     1681 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
     1741 cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac tggggctgac
     1801 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
     1861 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
     1921 ctggcaacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
     1981 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
     2041 cggcgaaacg cctagccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
     2101 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
     2161 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
     2221 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
     2281 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
     2341 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
     2401 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
     2461 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
     2521 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
     2581 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
     2641 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
     2701 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
     2761 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
     2821 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
     2881 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
     2941 cggcgctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
     3001 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
     3061 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
     3121 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
     3181 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
     3241 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acaccacctc
     3301 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
     3361 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
     3421 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
     3481 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
     3541 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
     3601 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
     3661 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
     3721 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
     3781 ggtttttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
     3841 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
     3901 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
     3961 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
     4021 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
     4081 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
     4141 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
     4201 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
     4261 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
     4321 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
     4381 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
     4441 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
     4501 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
     4561 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
     4621 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
     4681 caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
     4741 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
     4801 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
     4861 aataccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
     4921 cggctgacgc gagtcaaaaa ctttggcgca ttaccctgca cagaaaggcc aaacagcatg
     4981 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
     5041 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
     5101 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
     5161 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
     5221 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
     5281 accgccagcg acaaaatcag cccattccag ccggtggcaa agaaaccacc gtagcgccag
     5341 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
     5401 ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc ggtttcacca
     5461 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
     5521 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
     5581 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
     5641 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
     5701 atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
     5761 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
     5821 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
     5881 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
     5941 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
     6001 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
     6061 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
     6121 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
     6181 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
     6241 ggaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt tgtggattat
     6301 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
     6361 cggcggcgac cccggcgagg gcgataaagc cagtgcccgt cgccacggaa agatgaaagc
     6421 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
     6481 gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagata atcatcaacg
     6541 tcatcggcac catgagttta agcttatggt tggcgcgctc cagtagctcg aactgcccgg
     6601 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
     6661 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
     6721 gcgcattctc ggttttcagc attgacggtc cggtagagac tttaatgtcg gccacgtctg
     6781 ccagggtgat ttgctgcttc atcggcgtca ggatcggcaa ctggcgcagt gcctgcggac
     6841 tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc ccttccaccg
     6901 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
     6961 tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
     7021 ccagacgctc ggctagagca gaagccacgc ctggcaccgt tcgcgctact tcttcaattt
     7081 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
     7141 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
     7201 ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc attgtcatgc
     7261 ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc tccagcggag
     7321 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
     7381 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
     7441 ccggcagcgt cgatggcata tacaacaggt cgccttcatt gatctgcggt aaaaattccc
     7501 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc accaacagcg
     7561 tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga atcaaaaagc
     7621 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtaa cccatcagga
     7681 tagggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt ttggtgaacg
     7741 ccagcgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
     7801 acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat gcatcggtga
     7861 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
     7921 ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc gccccgacgg
     7981 caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag tgcatgacaa
     8041 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
     8101 gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc aacttgccgc
     8161 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
     8221 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt
     8281 ctcgggcgtt tttgccggat cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt
     8341 ttagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcgcgca
     8401 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
     8461 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
     8521 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga ctgatgccat
     8581 actgcgccag gcgctgggga tcgataacca cctgatactc tttcaccaca ccgcccaccg
     8641 acgccacttc cgcaacgtca gggatggttt tcagctcata tttgagaaac cagtcctgca
     8701 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
     8761 tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca ggcagcttac
     8821 cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga tcggtgccat
     8881 cttcgaaaat gacatacaca taagagtcgc caaactgtga gaaaccgcgc acagtcttcg
     8941 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
     9001 gtggtgcctg accgggatag ctggttttaa taataacctg cacatcggag agatccggca
     9061 gcgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
     9121 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
     9181 attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg cgccagaaat
     9241 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
     9301 accagagcgt aatgcggtga cgccttgcga tgcctggaaa acagcaacgc gtttcggtac
     9361 aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ctgccggtat caatcagcgc
     9421 ttgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
     9481 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
     9541 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
     9601 cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg
     9661 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca cgttatcttt
     9721 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt
     9781 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
     9841 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
     9901 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
     9961 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    10021 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    10081 gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca cccccagatt
    10141 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    10201 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    10261 gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcggg
    10321 tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat
    10381 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    10441 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    10501 agttttaatt tcactcattt tcgtctgcgg agtgatggta aagcgcatgg tcatctccgg
    10561 ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    10621 accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca tggtttcatg
    10681 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    10741 ttgcagtgct tttttcattt attctctcct ggagttaaaa gtcatactct aaataattcg
    10801 agttgcagga aggcgacaag cgagtgaatc cccaggagct tacataagta agtgactggg
    10861 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat tgctgtcaac
    10921 cgccacctaa ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga
    10981 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
    11041 ctgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
    11101 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    11161 cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa
    11221 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    11281 taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg
    11341 cggttgatat tccgctggtc agccttatag atgggaaaaa tgccgcgcgt gcggccccaa
    11401 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
    11461 agatttgcga cgacaatccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
    11521 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    11581 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttcaatc accccgcgag
    11641 cctgttccag cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat
    11701 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgctagct
    11761 gctgattgaa ataactttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg
    11821 cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga
    11881 aaaaatcgag atcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat
    11941 tccctttaag attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    12001 cggcatcggt cagacgatat tgcgcccgcg tttcctgcac tttcagcgtc gccatgcgca
    12061 aatcccggtt attcaccagc gcctcgctaa tcagcgtctt tacctgatta tcaacaaaaa
    12121 aggtgcgcca gcccacgttc tggtagttat ctgctgcgtt aaccaagccg ttctggctga
    12181 gtgagaactg ctgcggcacg ggcattgccg gacgctgata atccggtgcc agtgaacaac
    12241 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat
    12301 ttctggtgat tttataccgc caactttact cgccaggctc tgattttccg gtgacaggaa
    12361 aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat
    12421 tgcccgggca acatgcggag gaaaaatgaa actgttgatt gtcgaagatg aaaagaaaac
    12481 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    12541 cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa tcctcgatat
    12601 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg
    12661 gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga
    12721 gttgggggca gatgactacc tggtgaaacc attcgctttt gctgaactgc tggcgcgggt
    12781 gcgcactctc ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga
    12841 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    12901 cagtaaagag tttactctgc tggagttttt cctccgccat cagggcgaag tgctgccccg
    12961 ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac agcgacacta acgccattga
    13021 tgtagcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccaa agctaattca
    13081 gaccgtgcgc ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc
    13141 gcccgttttc gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcagcgt
    13201 ttttcgcctt tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata
    13261 ttaatgattt aaaagaaatt agcgccacgc ttgaacgggt acttaatcat cctgacgaga
    13321 cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga
    13381 tctccctggc agatagccac ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc
    13441 gcgagtttgc acgtgatgcc atacccgata aagacgcgcg gggtggcgag gtgtatctcc
    13501 tttccggccc gacgattatg atgccaggcc acggtcacgg gcatatggaa cacagcaact
    13561 ggcggatgat taacttgtcg gttggcccgt tggtggacgg caaaccgatt tatacgctct
    13621 acatcgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta
    13681 ttatgaccgc atcggtaatc agcatcctga tcgtctttat cgtactgttg gcggtacata
    13741 aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc
    13801 tcgacgttcg ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt
    13861 tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg
    13921 atatcgctca cgaaattcgc acgccgatta ccaatctcat cacgcaaacg gaaatcgccc
    13981 tcagccagtc tcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc
    14041 tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc
    14101 agctaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt
    14161 ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg atttgttggc gacgaatgtc
    14221 aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt tctaatgccc
    14281 tgcgttatac gccgcccgga gaggcaattg tagtgcgctg ccagacggtc gatcaccagg
    14341 tgcaagttac cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt
    14401 ttgaccgttt ctaccgcgtt gacccttccc gccagcgaaa aggcgaaggt agcggcattg
    14461 ggctggcgat agtgaaatcg attgttgtcg cgcataaagg cacggttgca gtaacgtcag
    14521 atgcgcgggg gacaaggttt gtgattatgc tgccggagag agagtgatta ttaagttcaa
    14581 attcagggcg tggtgcatca cctcctggta ttgttgatca acaaattaga gaaaaactta
    14641 ttaaacatat gaaatatcga aggcttttat ttttcaaagt ttcacgagat gtttgtatat
    14701 gaaacttagt taaatctgtg tttcttccaa ttgtagatca caatgccctt tctaaatttt
    14761 catcgttata aacaagccat ctatcatata aagcaatgaa taatttaatc
//
LOCUS       DAZFKM010000126        14614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFKM010000126 DAZFKM010000000
VERSION     DAZFKM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..454
                     /locus_tag="ACHKAY_003933"
     CDS             98..454
                     /locus_tag="ACHKAY_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5702523.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(505..1401)
                     /gene="yhaJ"
                     /locus_tag="ACHKAY_003934"
     CDS             complement(505..1401)
                     /gene="yhaJ"
                     /locus_tag="ACHKAY_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO5702524.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1506..2207
                     /locus_tag="ACHKAY_003935"
     CDS             1506..2207
                     /locus_tag="ACHKAY_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO5702525.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2230..2394
                     /gene="yhaL"
                     /locus_tag="ACHKAY_003936"
     CDS             2230..2394
                     /gene="yhaL"
                     /locus_tag="ACHKAY_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO5702526.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2606..3916)
                     /gene="cyuA"
                     /locus_tag="ACHKAY_003937"
     CDS             complement(2606..3916)
                     /gene="cyuA"
                     /locus_tag="ACHKAY_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO5702527.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3944..5275)
                     /locus_tag="ACHKAY_003938"
     CDS             complement(3944..5275)
                     /locus_tag="ACHKAY_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5702528.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5550..6914)
                     /gene="tdcG"
                     /locus_tag="ACHKAY_003939"
     CDS             complement(5550..6914)
                     /gene="tdcG"
                     /locus_tag="ACHKAY_003939"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5702529.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6986..7375)
                     /locus_tag="ACHKAY_003940"
     CDS             complement(6986..7375)
                     /locus_tag="ACHKAY_003940"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO5702530.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7389..9683)
                     /gene="tdcE"
                     /locus_tag="ACHKAY_003941"
     CDS             complement(7389..9683)
                     /gene="tdcE"
                     /locus_tag="ACHKAY_003941"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO5702531.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9717..10925)
                     /gene="tdcD"
                     /locus_tag="ACHKAY_003942"
     CDS             complement(9717..10925)
                     /gene="tdcD"
                     /locus_tag="ACHKAY_003942"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO5702532.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10951..12282)
                     /gene="tdcC"
                     /locus_tag="ACHKAY_003943"
     CDS             complement(10951..12282)
                     /gene="tdcC"
                     /locus_tag="ACHKAY_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO5702533.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12304..13293)
                     /gene="tdcB"
                     /locus_tag="ACHKAY_003944"
     CDS             complement(12304..13293)
                     /gene="tdcB"
                     /locus_tag="ACHKAY_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO5702534.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13392..14330)
                     /gene="tdcA"
                     /locus_tag="ACHKAY_003945"
     CDS             complement(13392..14330)
                     /gene="tdcA"
                     /locus_tag="ACHKAY_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO5702535.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3815 a         3902 c         3398 g         3499 t
ORIGIN      
        1 tcaaaacctt tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga
       61 atagactttc gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt
      121 aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct
      181 gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt
      241 tccattcctt tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt
      301 tcgtcgtctg cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat
      361 cattggctgg ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg
      421 ttacggaaac gatcctaagt ttggttcaaa ttaatcttag aattggggcg acatttcgcc
      481 cctttttatt aataataaca aatattattt tccgctaaaa agtttgggaa tttcccgcag
      541 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
      601 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
      661 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
      721 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
      781 acgagcggta tccgctaccg caattccacg atatttcacg cgcgtcactt cagataacgg
      841 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
      901 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacaat
      961 atccgcccgc ccctgctcca gtcgttccca cgcccccgcc agcacttcgg tgatgattgc
     1021 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
     1081 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gggcttccgc
     1141 atcggtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
     1201 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
     1261 ttcttccagt ttttgcatgg tgtagctaag tgcagaaggc acgcgtccca gctcatccgc
     1321 cgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
     1381 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
     1441 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
     1501 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
     1561 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
     1621 ccctgcgtgt gcttaaccag gaagtgctgg ccccagatgc cgcctttcag ccgcgaacct
     1681 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
     1741 aaggcaatca tgttcaggcc agcgccggtg aggcgttgct gctctctacc cagccgggtg
     1801 tcagctacag cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg cagctttggc
     1861 tggacgcctg cccgcagcga gagaatccgc tgattcaaaa actggcgctt aatatgggca
     1921 agcagcaatt aatcgcctcg ccagagggta cgatgggaag cctgcaatta cgccagcaag
     1981 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
     2041 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
     2101 aagcggcgct gacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
     2161 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
     2221 gagatgatga tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc cgtggtggcg
     2281 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
     2341 gccatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
     2401 gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa atccaacaac attaacgaat
     2461 taaacaaccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
     2521 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
     2581 cggcacgata cccaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
     2641 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
     2701 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
     2761 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg
     2821 caccatcgca aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatgg
     2881 tttcataacg cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg
     2941 ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat
     3001 gagaaagcat cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca
     3061 ccaccacagg cattgttgcg gtgatcccct ggttacccga gccggagtta ctcatagccg
     3121 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
     3181 aagagagatc tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat
     3241 gcagccccca cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat
     3301 cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca
     3361 gcgtcgttct ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct
     3421 gctgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
     3481 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
     3541 cattgcaagg ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat
     3601 cggcaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac
     3661 ctaacgcccc cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga
     3721 cgcccagacc gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac
     3781 cttccagttc tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc
     3841 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
     3901 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
     3961 acggagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
     4021 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
     4081 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc
     4141 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct
     4201 tctcggcagg catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
     4261 agcctaaata gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga
     4321 ccactttcac ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg
     4381 aaatattctg ctcataggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
     4441 cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
     4501 ctttatgccg cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc
     4561 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
     4621 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
     4681 tcgacacgcc cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt
     4741 tgaataacaa tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac
     4801 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag
     4861 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
     4921 agagcgcacc taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt
     4981 aatctttaca ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca
     5041 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
     5101 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
     5161 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
     5221 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
     5281 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
     5341 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
     5401 aatcttcaca atcaagactt tgaaataacg gagatggata agaattttct actaaattaa
     5461 tcgcaagaga atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt
     5521 gcacccaagg atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct
     5581 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
     5641 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg
     5701 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
     5761 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
     5821 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc
     5881 acgccaatct ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac
     5941 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
     6001 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
     6061 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg
     6121 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga aaccagctga
     6181 cgacgcagcg ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg
     6241 ttcatcccgc gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
     6301 atttccgctt tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
     6361 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac
     6421 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
     6481 gagtaatagg ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg
     6541 ttctcatggc gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca
     6601 acaatatgcg caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat
     6661 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg
     6721 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccatacag atcgaccaca
     6781 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
     6841 gcattcattg gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
     6901 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
     6961 ttgcgaccgt cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca
     7021 gcttcacatc tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct
     7081 gatgctcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
     7141 tgatgaacac ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg
     7201 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
     7261 tctgcgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
     7321 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
     7381 ctccggcgtc agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg
     7441 gtcagtgcgt tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
     7501 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
     7561 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
     7621 gttttacgta ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag
     7681 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca
     7741 cctttgcggt cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga
     7801 cgaccgtccg gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga
     7861 atcgactggg tagtgacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag
     7921 cgttcaacca ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga
     7981 tattcaccgt cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg
     8041 cgggcatatt tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat
     8101 gccatagtgc gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac
     8161 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc
     8221 atgaagtgat cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt
     8281 gctgttttcg gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag
     8341 agcagcgttt tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc
     8401 attgggctga cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga
     8461 tcgtcatttt catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg
     8521 aaggcaatcg gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc
     8581 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc
     8641 agccccatcc cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca
     8701 aattccggtg tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt
     8761 tcctgtgcct gctgctcatt gagtacgcca gctttaaagt cgcgctcaat gtagatatcg
     8821 aggaacgatg ccgtgcggcc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
     8881 agataagcga agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata
     8941 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc
     9001 agctcctcac gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg
     9061 agatcggcaa attgcagttc gcgttcacgt accagataac tgatgccata cagcgctacg
     9121 cggcgatagt caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg
     9181 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
     9241 gttttacgca gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg
     9301 tggaatgaac ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc
     9361 gcatccgttt gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga
     9421 gcggtaattg tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcgttttcg
     9481 atacggatac cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg
     9541 aggaaagatt catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta
     9601 atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg
     9661 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
     9721 gcaaattctg cgggcgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt
     9781 tcttcgttag tcggaataac agcgcaaatg acacgcgcat tttcactgga aacaattcgc
     9841 tcaccacagg agttagagcg attattcatt tctgtatcaa tctctacgcc taatacagcc
     9901 aaatgttcca tgaccagacg acgaattaag cttgaattct ctcctattcc gccggtgaat
     9961 ataattccat ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg
    10021 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    10081 agaacacgta aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact
    10141 acgcgttcca ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca
    10201 aagtcgacat cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt
    10261 gaagtatcaa cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc
    10321 gccacaacca ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa
    10381 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    10441 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    10501 aataccgcca cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa
    10561 ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    10621 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    10681 aaggccacac tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    10741 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    10801 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg
    10861 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    10921 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    10981 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    11041 agacggcgct ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg
    11101 tgcgcccatg gcttcaataa ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    11161 ggagcccatg atgaagatca tgctgatagt gttcagttta cccagcgaca ctttagtttt
    11221 gtcgccttta taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata
    11281 gtgaccgaag aaagatttga agatagccac gagtgcgatg atggaagccg catattccag
    11341 tgtaatcgcg aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga
    11401 aagcactgga atattctgcg ctttggcttc cgccatgttg gccggagaca gcgtaaacag
    11461 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat
    11521 ttgggaacat ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt
    11581 agaaaccacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    11641 ccccagccag acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc
    11701 aacctggtcg ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag
    11761 gctggcgata aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa
    11821 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    11881 tgcaaatccg agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata
    11941 aatccacagc agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    12001 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    12061 acacagacgc gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat
    12121 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    12181 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    12241 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    12301 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    12361 ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa
    12421 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    12481 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    12541 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc
    12601 ggtagttcgg tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    12661 ttcagactgt acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat
    12721 accagcaatt aaaccgccac caccaattgg cacaatcacg ttatcgacat catagagatc
    12781 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    12841 tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt
    12901 gaaattatca ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga
    12961 ttttggcgca ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag
    13021 cgaaacccct tgcgcatggt tgcccgcaga acaggccact acacctttac gtttttccgc
    13081 atcagtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg
    13141 catattttca aacttcagga atatttcacc tttgcaacgt tcactaaaat agtttgagcg
    13201 aggcatgcct gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatgtcatc
    13261 aatagcaacc ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat
    13321 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata
    13381 aaaacaaata actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa
    13441 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    13501 cataccacgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    13561 tttgaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    13621 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    13681 tttctttgta acgtagtaag aagctcgctg tagtacccca tattggtttg tggcaacacc
    13741 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    13801 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    13861 tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    13921 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    13981 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    14041 accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    14101 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    14161 ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg
    14221 gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact
    14281 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    14341 gttaattacc tcattgacgg catgaagtgt atcaaaatga aatgaacagg atatgtgcga
    14401 ccactcacaa attaactttc aatactttcc atggtatcgt tacgcaaatt aaataccttt
    14461 aaatatcaac aagttaaagt ataaaaatca gcataaaccc tgatttttgt caaaaaaaat
    14521 gacggggata accatatttt atggcgataa catcattcgt tatgtggtta atataaaaaa
    14581 ggggctgaga ccatatttca agcaattacc ggat
//
LOCUS       DAZFKM010000127        14567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFKM010000127 DAZFKM010000000
VERSION     DAZFKM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..5337
                     /locus_tag="ACHKAY_003946"
                     /pseudo
     CDS             161..5337
                     /locus_tag="ACHKAY_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            5560..5826
                     /gene="hipB"
                     /locus_tag="ACHKAY_003947"
     CDS             5560..5826
                     /gene="hipB"
                     /locus_tag="ACHKAY_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO5702536.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5826..7148
                     /gene="hipA"
                     /locus_tag="ACHKAY_003948"
     CDS             5826..7148
                     /gene="hipA"
                     /locus_tag="ACHKAY_003948"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO5702537.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            7460..7668
                     /locus_tag="ACHKAY_003949"
                     /pseudo
     CDS             7460..7668
                     /locus_tag="ACHKAY_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8100..8663
                     /gene="fimA"
                     /locus_tag="ACHKAY_003950"
     CDS             8100..8663
                     /gene="fimA"
                     /locus_tag="ACHKAY_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5702538.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            9024..9734
                     /locus_tag="ACHKAY_003951"
     CDS             9024..9734
                     /locus_tag="ACHKAY_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO5702539.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9776..12427
                     /locus_tag="ACHKAY_003952"
     CDS             9776..12427
                     /locus_tag="ACHKAY_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5702540.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            12441..12971
                     /gene="ydeS"
                     /locus_tag="ACHKAY_003953"
     CDS             12441..12971
                     /gene="ydeS"
                     /locus_tag="ACHKAY_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702541.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12984..13487
                     /gene="ydeR"
                     /locus_tag="ACHKAY_003954"
     CDS             12984..13487
                     /gene="ydeR"
                     /locus_tag="ACHKAY_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702542.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            13546..14460
                     /gene="ydeQ"
                     /locus_tag="ACHKAY_003955"
     CDS             13546..14460
                     /gene="ydeQ"
                     /locus_tag="ACHKAY_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702543.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         4096 a         3102 c         3617 g         3752 t
ORIGIN      
        1 aatctatcaa ttcgaaagta ttagcaaatt agccaaattg acaccttccc acctccaact
       61 aacactcaat acagacctct gacaatttaa taagtttcat ttttaatgtc tgacgactca
      121 tctctttcgt ggctgtattt tttgtatatg gagcgtttat atgaatagaa tctatcgcgt
      181 gatatggaat tgcactctac aggtatttca ggcctgctcg gaattaactc gcagggcagg
      241 taaaacatcg acggttaatt tgcgtaaatc ctctggactg acaacgaaat tcagtagatt
      301 gacgctgggt gttttgctgg cactaagcgg ttcagcgtct ggtgcaagtc tggaagttga
      361 taatgatcag attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg
      421 gtatggcact ggagtgctta atattttggc tggcggtaat gcctccttaa ccactattac
      481 taccagcgtc attggcgcta atgaggactc agagggcacc gttaatgttt tgggtggcac
      541 ctggcgattg tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac
      601 ggggacgctg aatattaaac agaagggtca cgtcgatgga ggctatttaa gattaggttc
      661 ttcgacagga ggcgtcggga cggtcaatgt tgagggagag gactctgttc tgacgaccga
      721 attattcgaa atagggagtt acggtacggg ctcattaaat attacggata agggttacgt
      781 cacgagttca atcgtcgcca ttttaggcta tcaggcgggc agtaatggcc aggttgtcgt
      841 tgaaaagggt ggcgagtggc taataaaaaa taacgattcc tcaattgaat ttcaaattgg
      901 taatcaagga actggggagg cgactattcg cgagggtgga ctgattacgg ctgaaaatac
      961 gattatcggt ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc aagactctgt
     1021 catcacggta cgcagactct ataatggata tttcggtaat ggcacactca atatttccaa
     1081 taatggacta attaataaca aagaatattc attagtgggc gttcaggacg gttcccacgg
     1141 tgtcgtcaac gtgaccgata aagggcattg gaatttcctc ggaacgggcg aagctttccg
     1201 ctatatctat atcggtgatg ctggcgacgg tgaacttaat gtctcgcgtg aaggtaaagt
     1261 agattcggga attatcactg cggggatgaa agaaacaggc acaggcaaca ttactgttaa
     1321 ggataagaac tccgttatca ctaatctcgg aactaatctt ggttatgacg gccacggcga
     1381 aatgaatatc agtaatgaag ggcttgttgt cagcaacgga ggaagctcac tcggttatgg
     1441 agaaaccggc gtcgggaatg tcagcatcac cacggggggg atgtgggagg tcaataagaa
     1501 tgtctatacc accattggtg ttgcgggcgt cggaaacctc aatattagcg atggcggtaa
     1561 gttcgtatcg caaaatatta cttttttggg cgataaagca agcggtatcg gcacactgaa
     1621 cctgatggat gcgacatcat cgttcgatac tgtgggtatc aatgtcggta attttggtag
     1681 cggtatcgta aatgtcagta atggtgccac ccttaattca acgggctatg gatttatcgg
     1741 aggaaatgcc tccggtaagg ggatagttaa tatttcaacg gacagtctct ggaatttaaa
     1801 gacatcatct actaacgcac aattgctaca ggtcggtgta ttaggcaagg gtgaactgaa
     1861 tattaccacc ggaggtatag ttaaagcgcg tgatacacag atagctctca atgacaaaag
     1921 taagggcgac gtgagggtgg atgggcagaa ctctcttctt gaaacattca atatgtacgt
     1981 agggacatct ggtacgggta cgttaaccct gacgaatagc ggcacgctga atgtcgaagg
     2041 tggagaagtt tacttaggtg tttttgaacc tgctgtagga acgctaaaca ttggtgcagc
     2101 tcacggtgag gcggcggcag atgccgggta tataaccaac gcgacgaaag tagagtttgg
     2161 ttctggcgaa ggtgtttttg tctttaatca tactaataac agtgatgccg gctaccaggt
     2221 cgatatgctg attacaggtg acgataaaga cggaaaagtg atccatgatg caggccatac
     2281 ggtgttcaat gcagggaata cttatagcgg taaaacgctg gtcaatgacg gcctcctgac
     2341 cattgcgtct catacggcag atggggtaac gggcatgggt tcgagtgaag tcaccattgc
     2401 aagccccggt acgctcgaca ttctcgcatc aacgaacagt gcaggagatt acacgctgac
     2461 caatgcgctc aaaggcgatg gcttgatgcg agtgcagctg tcatcctacg acaagatgtt
     2521 tggctttacc catgcaacag ggactgaatt cgccggtgtt gcccaactga aagacagtac
     2581 cttcactctg gaacgcgaca acaccgctgc gcttactcac gcgatgttgc agtctgacag
     2641 tgaaaatacc acatcggtaa aagtgggaga gcaatccatt ggtggactgg ccatgaatgg
     2701 cggtacgctc attttcgata cggatattcc tgctgcgacg cttgcagagg gatatatcag
     2761 cgtcgatacg ctggttgtcg gcgcgggtga ctacacctgg aaaggccgta actatcaggt
     2821 aaacgggacg ggcgacgtgc ctaaaccgtg gaatgatccc atggcgaata accctctgac
     2881 gacgctcaat ttgctggaac acgatgatag ccatgtcggc gttcaactgg tgaaggcgca
     2941 aacggttatt gggtcgggtg gctcattaac gttacgtgat ttacagggcg acgaggtgga
     3001 agcggacaaa acgttacaca ttgcgcaaaa cggaacggtg gtcgccgagg gtgattatgg
     3061 attccgcctc acgaccgcac caggtgatgg tttgtacgtt aactatgggc tgaaagcgct
     3121 gaacatccat ggtgggcaaa agctgacgtt agccgaacat ggcggagcct atggcgcaac
     3181 ggccgatatg tcggcaaaaa tcggtggtga aggggatctg gcaatcaata cggtgcgaca
     3241 ggtttcgctt tccaacggtc agaacgacta tcagggggca acctacgttc agatggggac
     3301 attacgtacc gatgcggatg gtgcacttgg caacacccgg gaactgaaca tcagcaacgc
     3361 ggccatcgtc gatcttaatg gatcgacgca gacggtagag acattcaccg ggcagatggg
     3421 ttcgactgtt ttgttcaaag agggggcgct gacggtaaat aaaggtggga tcagtcaggg
     3481 tgaactgaca ggtggcggaa acctgaatgt tacaggggga acgctggcta tcgaggggct
     3541 taatgcacgc tacaatgcgt taaccagcat tagcccaaat gcggaagtca gcctcgataa
     3601 tacgcagggg ttaggcagag gaaatattgc caataacggt ctgttaacgc taaaaaacgt
     3661 gactggcgaa ctgcgcaata gcataagcgg gaagggtatc gtgagcgcaa ccgccaggac
     3721 agatgtagag ctggatggcg ataatagccg ctttgtgggg caattcaaca ttgatacagg
     3781 cagcgcgctc agcgtcaacg agcagaaaaa cctgggtgat gcttccgtta tcaataatgg
     3841 cctgctcacc atctccactg agcgtagctg ggcgatgacg cacagtatca gcggtagcgg
     3901 tgatgtgaca aaactgggta ccgggatcct gactcttaac aacgattccg cggcgtatca
     3961 gggtacgacg gatatcgtgg gggggggaaa ttgctttcgg ttccgactct gccattaata
     4021 tggcaagtca acacattaat atccataaca gcggtgtgat gtcgggaaat gtcaccactg
     4081 caggtgatgt gaacgttatg cctgggggga cactgcgtgt cgctaaaacc acaatcggcg
     4141 gcaacctgga gaatggcggc acggttcaaa tgaatagcga aggggggaaa ccggggaatg
     4201 tactgaccgt taacggcaac tataccggaa acaatggcct gatgacgttc aacgcgacgc
     4261 tgggcggcga taattcgccc accgataaaa tgaacgtgaa aggcgatacc caagggaaca
     4321 ctcgcgttcg ggttgataac attggcggcg tcggtgcaca aacggtcaac ggtattgaac
     4381 tcattgaggt tggcggtaat tctgcaggta acttcgcgct gaccaccgga actgtcgaag
     4441 ctggggctta cgtctacacg ctggctaaag ggaaggggaa tgacgagaaa aactggtatc
     4501 tgaccagtaa atgggacggc gtaacgccag cggatacacc cgatcccatc aataatcccc
     4561 ctgttgtgga tccggaaggc ccatcagttt atcgcccgga ggccggaagc tatatcagca
     4621 acattgccgc agccaactcg ctgtttagcc atcgtttaca cgaccgtctg ggtgagccgc
     4681 agtatataga ttcactgcat tctcaggggt cggcaagcag tatgtggatg cgtcatgtcg
     4741 gagggcacga acgttcaagg gccggtgacg gtcagctaat tactcaggct aaccgctatg
     4801 tattgcagct aggcggcgat ttggcgcagt ggagtagcaa cgcgcaggat cgctggcatg
     4861 acggcctctg ttgagggtgg gtataccttt gaagcgggaa catttagagg cagcgaaggg
     4921 acgctgaata cctggtacgt ccagccacag gcgcaaatca cctggatggg tgtgaaagat
     4981 tctgaccata atcggaaaga cggaacacgc attgaaacgg aaggcgacgg aaacgtgcaa
     5041 acgcgacttg gggtgaaaac ctacctgaat agccatcaca agcgtgacga tggtaaacag
     5101 cgtgagttcc agccttacat tgaagcgaac tggatcaaca atagcaaagt ctacgccgtg
     5161 aagatgaatg gtcaaaccgt aagccgtgat ggtgcgcgta atctcggtga agtacgtacc
     5221 ggggttgagg cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg tgtgcaacta
     5281 ggtgataaag gctatagcga tactcagggc atgctgggag tgaaatatag ctggtaaacc
     5341 gtataagccg catgtcgaga tggcatgcgg cttaatattg ccgacttcaa acggcgcatc
     5401 agcgcctttt ttaaatcctc ctttttatcc gcgatcgcgg atatcgcagc gtttatcccg
     5461 tagagcggat aagacgtgtt tccaaattga cttatcctca ctaaaggata aaactgataa
     5521 tatcctctta tgcggataaa tttgctgtgg acgtatgaca tgatgagctt tcagaagatc
     5581 tatagcccaa cgcaattggc gaatgcaatg aaactggttc gccagcaaaa tggctggacg
     5641 cagagcgagc tggcgaaaaa aattggcatt aagcaggcga caatttccaa tttcgaaaac
     5701 aaccctgaca ataccacgct cacgacattt tttaagattt tacagtcgct tgaactctca
     5761 atgacactat gcgacacgaa aaatgcctcg ccagaatcaa cagaacagca agatctggag
     5821 tggtaatgcc taaacttgtc acttggatga acaaccagcg ggtaggcgag ttaacgaagt
     5881 tagccaacgg cgcgcacacc tttaagtatg caccggagtg gttagccagc cgttatgcca
     5941 gaccgttgtc actttcgctg ccattgcaga gggggaatat cacctcagat gccgtattta
     6001 acttcttcga taacctgtta cccgatagcc cgattgtacg tgaccggatc gttaaacgtt
     6061 atcatgctaa atccagacaa ccgtttgatt tattgtcaga aatagggcga gacagcgttg
     6121 gtgccgtaac gttattaccc gaaaatgaaa ccataacgcg cccgataatg gcatgggaaa
     6181 agcttactga agccaggctt gaagaagtat taacggctta taaagcagat atccctctgg
     6241 gcatgattag agaagaaaat gactttcgca tctcggttgc tggcgcgcag gagaagacag
     6301 cgctgcttag aataggcaat gactggtgca ttccgaaagg aataacgccg acgacgcaca
     6361 tcattaaatt accgattggc gaaatcagac agcccaatgc gacgctcgat ctcagccaaa
     6421 gcgttgataa tgagtattac tgtctgctgc tggcgaaaga acttgggttg aatgttccgg
     6481 acgcagaaat cattaaagcg ggaaatgtgc gcgcgttagc ggtcgaacgt tttgacaggc
     6541 gttggaatac tgagcgaacg gttttacttc gcttgccaca ggaggatatg tgtcagacat
     6601 tcggtttacc ttcatctgtg aaatatgaat cagatggagg cccaggcatc gcgcggatca
     6661 tggcgttttt gatggggtcc agcgaggcgc ttcgcgatcg ttatgatttt atgaaattcc
     6721 aggtcttcca gtggttgatt ggcgcaacgg atggccatgc aaaaaacttc tccgtattta
     6781 ttcaggctgg cggcagttat cgactcacgc cattttacga catcatttca gcatttccgg
     6841 tccttggcgg tacgggaata cacatcagcg atctcaaact ggcaatgggg cttaacgcat
     6901 ccaaaggcaa aaaaacggca atcgataaaa tttatccgcg acattttttg gcgacagcaa
     6961 aggtgctgag attcccggaa gtgcagatgc atgaaatcct aagtgacttt gccagaatga
     7021 ttccggcagc actggataac gtgaagactt cattaccgac agattttccg gagaacgtgg
     7081 tgacggcagt tgaaaccaat gtgttgaggt tgcatggacg gttaagccga gaatacggta
     7141 gtaagtgaga tatggggttt ggtcgttgtt aatgagcacg acaatcatga ccgccaacga
     7201 ctggattctt ccttggttta tactggtgcg cataatgtat attatgttaa attatgtctg
     7261 agtgatgcga agtgcttttc tggtagtcgt tattcgttca gggttcaaga acttaagcct
     7321 ctcctctttc ataattggcc tgtgcatgga gaaatagcct ggttattccc tgccccatgc
     7381 taagagcgca gtgtctgtcg catagacact gcatacgaaa acaccagagg gtgctgttac
     7441 ttatttaagg acggatcaag cgggaatgag tccactccgg tatcagaaat ggctacgtct
     7501 caatgaagtc aggcgaccga tgctgaatga acattacgat gtcaccacag cagcctatgc
     7561 tgtcggttac gaaagctatc ccatttcagt cgggaatatt cgcggatgtt tggagagtca
     7621 cccaagagag atattaccag gttaagaaag tctgtcggtc agctttaata aaccctgcac
     7681 ttatctgttt agagcaattc ggtgttagtt tcagcaagca aacattaacc atagctaatg
     7741 atttatagcc atattaacca ttggtagccc ttgacctttc ctgacacttc ccttgttcgc
     7801 aataatattc tgtttagtca cggtttataa ataacatgca taatgaaatc gtcatgttat
     7861 ttagcgacat aaaatgtttt tgcaaatact ctcttcaaca agtgcacaat aaacttgttg
     7921 tgattaggtg tccttgtgat ttaaagatat tatcgttctt cgtgaaatgt aataaccctg
     7981 gaaactatat tctcagggca acaaccaatc aagaatgatt cttgttttta agcaataaca
     8041 gaatgactta attgttaacc ctctttattc agctacttaa atataaattt tggagaatta
     8101 tgaaactcaa acatgttggt atgattgtcg tttctgtgtt ggcgatgtcg tctgctgcgg
     8161 taagcgcagc cgagggtgat gaatcagtaa cgaccactgt taatggcggt gttattcatt
     8221 ttaaaggtga agtggtaaat gccgcttgtg caattgattc agaatcaatg aaccaaacgg
     8281 ttgagctggg tcaggttcgt tcttctcgct tggctaaagc gggtgacctc agctccgccg
     8341 ttggcttcaa tatcaagctg aatgattgtg ataccaatgt ttccagtaat gcagctgttg
     8401 cattcctggg tactactgtc accagtaatg acgatacgtt agcgctgcag agttcagcgg
     8461 caggctctgc ccaaaatgtc ggtattcaaa ttttggaccg tacgggtgag gtattaatac
     8521 ttgatggggc cacttttagt gctaaaaccg acttgattga tggcacgaat atactgccat
     8581 tccaggctcg ttatattgct ctcgggcagt ccgtagctgg tactgcaaac gctgatgcga
     8641 ccttcaaagt tcaatatcta taattcaatc ttaaggtggc tatacgtcat ctgaaattga
     8701 aacagggtgt gtgtggcatg gctacgcaca cccgagcaat ttaccaatat tcatctcatt
     8761 tttaatgaga aatggtttgt atttggagcg ctatgaaaaa tcacataata tctaaactat
     8821 caataaacaa cagtagttaa acttactgtt atttaaaata tttactttgt agcaaggttt
     8881 tcctattaaa agagaaccaa catttattac aaacgcatta agtctggaaa cccggcgact
     8941 cgccaatgta ttagcaacat taatcgattg tctgatgtaa cggttttccc gcttatcctc
     9001 ttaacacatg gagaatgcat cagatgcaaa ctacgcgtac cccctactct atttccttta
     9061 tggcgacagt gttactttta ttgctctttg cttgccatag caccgttgcc aacgcagctg
     9121 tcgcactggg tgcgacgcgg gtaatttatc cggcaaacca gaaacaggtt ttattgcctg
     9181 tcacgaataa tgatcctgcc agtgtctatc tcattcagtc atggatagaa aatgcaggtg
     9241 accaaaaaga cacccagttt gtcattaccc caccgctttt ttccatgcaa ggtaaaaaag
     9301 agaatacgct gcggataatc aacgctacta atcatcagtt accaggcgac cgtgaaagcc
     9361 tgttctgggt gaatgttaaa gctattcctg ctatggaaaa agaccagaag aatgagaata
     9421 cattacagct ggcaattatt agccgcataa aaatgtttta tcgccccacg aacctggcaa
     9481 tggcacctga agaagcgcct gcaatgcttc ggtttcgtcg cagcggtagc aaactcacgc
     9541 ttattaatcc aacgccctat ttcattaccg taacaaatat gaaagcagga aatagcaatc
     9601 tcccaaacac catggtgccg ccaaaaggtg aagtatcggt ggatatcacg catgctgcaa
     9661 ccggggatat cagttttcag acgatcaatg attacggtgc attaactccg cgtattaagg
     9721 caaccatgca gtaagccagt gacctaatta ataataaact caatcggaat taataatgac
     9781 agcctttcgt gccgcattta aggcatatcg tatgcatcag gtgttaataa tgcctcgctt
     9841 tgcacggctt accattgccc ttggcttagc aactgcggta tttcccgttg acgcagagta
     9901 ttattttaat ccacggtttt taagcaatga tcttgctgaa tctgtcgatt tatccgcctt
     9961 tactaaggga cgcgaggcac cacctggtac atatcgggtc gacatttatt tgaacgacga
    10021 atttatgact agtcgggata ttacttttat tgcagatgat aataacgcag aactcatccc
    10081 atgcctgagt acagatcttc ttgtcagtct tggtataaaa aaaatagcgt tattagacaa
    10141 taaagaacat tcggcagaaa aacatgtgcc ggacaacagc gcatgcacac cacttcagga
    10201 tcgtttggcc gacgcatcaa ctgagtttga tgttggtcag caacatttgt cactctccgt
    10261 tccgcaaatt tatgttggca gaatggctcg cggctatgtt tctcctgatc tgtgggaaga
    10321 aggaataaat gctgggcttc taaactatag ttttaatggt aattctatta ataatcgtag
    10381 taaccataat gcaggaaaat ccaactatgc atatttgaat ttacagagtg gcatcaacat
    10441 tggtagttgg cgactgcgcg ataactcaac gtggagttat aacagtggga gtagcaattc
    10501 atctgacagc aataaatggc agcatatcaa tacgtcggct gaacgtgaca ttattccctt
    10561 acgctcacgg ttaacggtag gtgatagtta taccgatggc gatatttttg atagtgtgaa
    10621 ctttcgtggc ctcaaaataa attcaacaga agcgatgttg cccgatagcc aacatggttt
    10681 cgctccggtg attcatggta ttgcccgtgg aaccgcacaa gtgagtgtaa aacagaacgg
    10741 atacgatgtt tatcagacta ctgttccacc cggccctttt actattgatg atatcaactc
    10801 tgcggccaat ggtggtgatc tgcaagtaac cataaaagag gcagacggca gtattcagac
    10861 attatatgtt ccttattcgt ctgttccggt tctccaacgt gctggatata cgcgttatgc
    10921 gcttgccatg ggggaatatc gtagtggaaa taacctgcaa agctctccca agttcataca
    10981 aggtagcttg atgcatggtc tggaaggaaa ctggacacct tatggcggaa tgcaaattgc
    11041 agaagattat caggccttca accttggtat tgggaaagat ttaggacttt ttggtgcctt
    11101 ttctttcgat atcacgcagg ccaatacgac acttgcagat ggcacccgtc acagtgggca
    11161 atcggttaaa tccgtctaca gcaaatcctt ctaccagaca ggaaccaata tccaggtcgc
    11221 aggatatcgc tattctacgc aaggttttta taacttatcc gacagtgcct acagtcgaat
    11281 gagtggttac accgtcaagc ctcctaccgg agacaccaat gagcagacac aatttattga
    11341 ttattttaat ctgttctaca gtaagcgtgg tcaggaacaa ataagcatct ctcagcagct
    11401 tggaaattac ggtacgacat ttttcagtgc cagtcgccaa agttactgga acacgtcacg
    11461 cagcgaccag caaatatcat ttggattaaa tgtgccgttt ggtgatatta cgacttcgct
    11521 gaattacagc tattccaata atatatggca aaacgatcgg gatcatttac tcgcttttac
    11581 gcttaatgtt cccttcagtc attggatgcg tacagacagt cagtcggcat ttcgtaattc
    11641 aaacgccagt tacagtatgt caaacgattt gaaaggcggc atgaccaatc tatcgggggt
    11701 ttatggcact ctgctgccgg ataataacct gaattatagc gttcaggtcg gtaacaccca
    11761 cggaggtaat acatcgtctg gcaccagtgg ttacagttct cttaattatc gtggagctta
    11821 tggtaatact aatgtcggtt acagtcggag tggtgacagc agccagattt attacggaat
    11881 gagtggtggg attattgctc atgctgatgg catcaccttt ggacagccgc tgggcgacac
    11941 aatggttctg gttaaggctc ctggtgctga taatgtcaaa atagagaacc agaccggaat
    12001 tcataccgac tggcgtggct atgccatatt accatttgcg acagaatata gagaaaaccg
    12061 tgttgctctt aacgcgaatt cccttgcaga taatgttgaa ctggatgaaa ccgtgatcac
    12121 tgtcatccca actcacggtg ctattgccag agcaacattt aatgcacaaa tcggcgggaa
    12181 agtattaatg acgttgaagt acggtaataa gagtgttcca ttcggtgcaa ttgtcacaca
    12241 cggagagaat aaaaatggca gcattgtcgc agaaaacggt caggtttatc tgactggact
    12301 tccacagtca gggaaattac aggtttcatg gggcaaagat aaaaactcaa actgtattgt
    12361 cgagtacaag cttcctgaag tttctcctgg taccttactg aaccagcaga cagcaatctg
    12421 tcgctaagga acaatagatt atgaaataca ataacattat tttcctcggt ttatgtctgg
    12481 ggttaaccac ctattctgct ttatccgcag atagcgttat taaaattagc gggcgcgtcc
    12541 tcgattatgg ctgcacagtc tcatcggatt cgcttaattt taccgtagat ctccaaaaaa
    12601 acagtgccag acaatttcca acgaccggta gcacaagtcc agccgtccct tttcagatta
    12661 cgttaagtga atgcagcaaa gggacaacgg gggttcgggt tgcatttaac ggtattgagg
    12721 atgcagaaaa taatactttg ttgaaactgg atgaaggaag caatacggcc tccggtttgg
    12781 gtatagaaat attggacgca aatatgcgtc cggtgaaact gaatgacctt catgccggga
    12841 tgcagtggat cccactggta ccagaacaga acaatatttt gccttactcc gctcgtctga
    12901 agtcaactca gaagtccgtc aatccgggac tggtgagggc ttcggcaacc tttacccttg
    12961 aatttcaata atcagagtca gatatgaaaa gacttcacaa gaggttcctg ttagctacgt
    13021 tttgcgcgtt attcacagca actctccagg ccgccgatgt cactatcact gttaatggtc
    13081 gggtagtcgc taaaccctgc actattcaaa ccaaagaagc taacgttaat ctcggggatc
    13141 tttatacgcg caatctgcaa caacctggtt ctgcatctgg ctggcacaat attactttgt
    13201 cattaaccga ttgtccggtt gaaacaagtg cagtgacggc aatcgtgaca ggttcaactg
    13261 acaatacggg ttattacaaa aatgaaggta ctgccgaaaa tattcagata gagctgaggg
    13321 atgaccagga tgctgcgtta aaaaatggcg atagcaaaac ggttattgtt gatgagatca
    13381 ctcgtaatgc acagtttcca cttaaggcaa gagctatcac ggtgaatgga aacgcaagcc
    13441 agggaacgat cgaggcgcta atcaatgtga tctacacctg gcaataacgt cgattcgatt
    13501 ttcttagcct tctttttaaa tcaattttta accttaggat atatcatggg taaaacaatc
    13561 agtataaagg tcctgttcgg tatttatctt ctattaatgg caggcaaagt ttttgccttc
    13621 agttgtaatg tcgatggcgg gtcaagtatt ggagcaggta caacctctgt ttatgttaat
    13681 ctcgaccctg taatacagcc gggccagaat ctggttgtag acttgtctca gcatataagt
    13741 tgctggaatg attacggcgg ctggtacgac actgatcata taaacctggt acaaggctca
    13801 gctttcgcag gctccctaca gtcatataaa ggatcgctgt actggaataa tgtgacctac
    13861 cccttcccgt tgacgacaaa taccaacgta ttggatattg gtgataagac accgatgcca
    13921 ttaccgttaa aactctatat taccccagtt ggggcggccg gaggagttgt cataaaagcg
    13981 ggggaagtca ttgcccgtat acatatgtat aaaattgcaa cattaggaag cggaaatccg
    14041 cgtaatttta cgtggaatat tatttcaaat aacaatgttg ttatgccaac aggcggctgc
    14101 accgttgata gtcgtaatgt caccgtggat ctcccggact ttcctggaag cgcagaaata
    14161 ccccttggcg tatattgctc aagcgaacaa aagctctcat tttatctttc gggtgccacc
    14221 actgacagta gccgacaagt gtttgctaac acagcacctg atgcaacaaa agcatcggga
    14281 gtgggtgtca ctctaatgcg taatggaaaa atactggcta cgggagaaaa tgtctccctg
    14341 gggacagtaa ataaaagcaa agtacctctg ggtttaagcg caacctatgg acaaacagga
    14401 aacaaggttt cagccggaac cgtccagtca gtcattggtg tcacttttat ctatgaataa
    14461 ttgtgatttt gaccttagtc tatgcgatcc attcacgaaa attgcgcgga taattatttt
    14521 gtgaaggcta ttagcctaca cctgtaagaa aatccgcgca caattga
//
LOCUS       DAZFKM010000128        14461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFKM010000128 DAZFKM010000000
VERSION     DAZFKM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..626
                     /locus_tag="ACHKAY_003956"
     CDS             60..626
                     /locus_tag="ACHKAY_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702544.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            677..3136
                     /locus_tag="ACHKAY_003957"
     CDS             677..3136
                     /locus_tag="ACHKAY_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO5702545.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3151..3882
                     /locus_tag="ACHKAY_003958"
     CDS             3151..3882
                     /locus_tag="ACHKAY_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO5702546.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3879..4940
                     /locus_tag="ACHKAY_003959"
     CDS             3879..4940
                     /locus_tag="ACHKAY_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702547.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5092..6138
                     /locus_tag="ACHKAY_003960"
     CDS             5092..6138
                     /locus_tag="ACHKAY_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO5702548.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6135..6926)
                     /gene="nei"
                     /locus_tag="ACHKAY_003961"
     CDS             complement(6135..6926)
                     /gene="nei"
                     /locus_tag="ACHKAY_003961"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO5702549.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6962..7696)
                     /gene="pxpA"
                     /locus_tag="ACHKAY_003962"
     CDS             complement(6962..7696)
                     /gene="pxpA"
                     /locus_tag="ACHKAY_003962"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO5702550.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7686..8618)
                     /gene="pxpC"
                     /locus_tag="ACHKAY_003963"
     CDS             complement(7686..8618)
                     /gene="pxpC"
                     /locus_tag="ACHKAY_003963"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO5702551.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8612..9268)
                     /gene="pxpB"
                     /locus_tag="ACHKAY_003964"
     CDS             complement(8612..9268)
                     /gene="pxpB"
                     /locus_tag="ACHKAY_003964"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO5702552.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9291..10034)
                     /gene="ybgI"
                     /locus_tag="ACHKAY_003965"
     CDS             complement(9291..10034)
                     /gene="ybgI"
                     /locus_tag="ACHKAY_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO5702553.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10305..11786
                     /gene="dtpD"
                     /locus_tag="ACHKAY_003966"
     CDS             10305..11786
                     /gene="dtpD"
                     /locus_tag="ACHKAY_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO5702554.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11828..13246)
                     /gene="phrB"
                     /locus_tag="ACHKAY_003967"
     CDS             complement(11828..13246)
                     /gene="phrB"
                     /locus_tag="ACHKAY_003967"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO5702555.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13243..13752)
                     /locus_tag="ACHKAY_003968"
     CDS             complement(13243..13752)
                     /locus_tag="ACHKAY_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO5702556.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13903..14461)
                     /locus_tag="ACHKAY_003969"
                     /pseudo
     CDS             complement(13903..14461)
                     /locus_tag="ACHKAY_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3484 a         3861 c         3511 g         3605 t
ORIGIN      
        1 atcaaataag atattcttgc accgatatac ttttttcttt tctgttaaag gactccgtta
       61 tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc tctgctgttc acagcacctg
      121 tttatgcggc tgatgaagga tccggtgaaa ttcactttaa aggtgaagtt attgaagcac
      181 cgtgtgaaat acatcaggat gatattgata aagaggttga actcggtcag gtgaccacca
      241 gccacattaa tcagtcacat cacagcgatg ccgttgctgt cgacctgcgc ttagtcaact
      301 gtgatctgga aaactccagc aacggttccg gtggcaagat ttctaaagtt gcagtcacct
      361 ttgatagctc agcgaaaacc accggcgcag atccgattct caacaacacc agcacgggtg
      421 aagccaccgg cgtcggcgta cgtttaatga ataaagatca aagcaatatc gttttaggca
      481 ccgctactcc agatatcgac ctggctccga cctccagcga acaaacgctg aatttctttg
      541 cctggatgga acaaattgat caggctacac ctgtaacgcc aggcgcagtt acggcgaatg
      601 caacctacgt gctggattat aaataagtat tactttggcg gcgctatcgc cgcctttcat
      661 aaaatattta tcaggtatgg acacagtgaa tatttatcga ctctcttttg tatcctgcct
      721 ggtcgtggcg atgccttgcg cattggcggt cgaattcaac cttaatgttc tcgataaatc
      781 aatgcgcgac cgcattgata tttcattatt aaaggaaaaa ggagtcattg ctcccggtga
      841 gtattttgtt agcgttgcgg taaataataa ccaaatcagt aacgggcaaa agattaactg
      901 gcacaaaaat gacgataaaa ccattccgtg catcaatgat ttactggtcg ataaatttgg
      961 cttaaaacct gaagtccgtc agtcgttacc attgataaat cagtgcgtcg attttagctc
     1021 ccgacctgaa atgctcttca atttcgatca agccaatcag caactaaata tcaccattcc
     1081 gcaagcctgg ctggcgtggc actcagaaaa ctggacccca ccctccacat ggaaagaagg
     1141 tgtcgccggt gtcctgatgg attacaactt gtttgccagc agctaccgcc cacaggatgg
     1201 cagcagcagc actaatctta acgcctatgg aacagccgga atcaacacgg gggcatggcg
     1261 cttacgcagt gattatcagt tgaatcagac tgatagcgat gataaccatg aacagtcagg
     1321 cgaaatatca cgtacctatc tttttcgccc gttaccgcaa ttaggttcaa aattaaccct
     1381 cggcgaaacg gattttagtt ccaatatttt cgacggtttt tcttataccg gcgcggcact
     1441 ggcaagtgac gatcgaatgt tgccatggga actacgcggc tacgccccac aaattagcgg
     1501 tattgcacag accaatgcca cggtgacgat cagtcaatca ggccgcgtca tttaccagaa
     1561 aaaagtccca ccaggcccat ttatcattga cgaccttaat cagtctgttc agggcacact
     1621 ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat ttccaggttt cggcagcatc
     1681 gacgcccttc ctgactcgtc agggacaggt tcgctataaa ctggccgcgg gtcagccacg
     1741 gccctccatg tcacatcaaa ctgaaaatga aacctttttt agcaatgatg tttcctgggg
     1801 gatgctgtca aacacctcgc tgtacggcgg cctgctgctt tctggtgatg actaccattc
     1861 tgccgcaata ggtatcgggc aaaatatgct gtggcttggc gcgctgtcgt ttgatgtcac
     1921 gtgggccagt agtcactttg atactcagca ggacgagcgg ggcttaagct accgttttaa
     1981 ttacagcaaa caagtggatg ccaccaacag cacgatttcc ctcgccgctt atcgtttctc
     2041 cgatcgtcat tttcacagct acgccaacta tcttgatcac aaatacaacg acagcgatgc
     2101 gcaggacgaa aaacagacga tcagcttatc tgtgggccaa ccgattaccc cactaaacct
     2161 caatctttac gccaacctgc tacatcaaac ctggtggaat gcagacgcct ccacgaccgc
     2221 caacatcacg gcaggtttta atgttgatat tggtgactgg agagatatct ctatttcgac
     2281 gtcattcaat acaacccatt acgaagataa agatcgcgac aaccagattt acctgtcgat
     2341 ttcgctcccc ttcggtaacg gtggtcgggt tggctatgac atgcaaaaca gtagccacag
     2401 caccacacac cgcatgtcgt ggaacgatac gctggatgaa cgtaatagct ggggcatgtc
     2461 tgccggactg caatccgacc gtccggacaa tggagcccag gtgagcggta actatcagca
     2521 tctgagttca gcgggtgagt gggatatttc tggtacctat gccgccaatg attacagttc
     2581 cgtcagcagc agctggagcg gttctttcac cgcaacccaa tatggtgcag cgtttcatcg
     2641 ccgcagctcc accaatgaac cacgcctgat ggtcagcacc gatggcgtgg cagatattcc
     2701 ggttcagggc aatctcgact acaccaacca ttttggcatt gcggtggtgc cgttgatttc
     2761 cagttaccag ccttccaccg tggcggtgaa catgaatgac ttacccgacg gcgtaacagt
     2821 tgcagaaaac gttatcaaag aaacgtggat tgaaggcgcg ataggttaca aatcactggc
     2881 ttcccgttcc ggtaaagacg ttaacgtcat cattcgcaac gccagcggtc agtttcctcc
     2941 cctcggagcg gatatccgcc aggatgacag cggcattagc gtggggatgg ttggcgagga
     3001 aggacatgcc tggttaagcg gagtcgctga aaatcaaaag tttaccgtgg tctggggtga
     3061 tagccagcat tgctcgctcc atcttcctga acatatggaa gacaccgcaa atcgcctgat
     3121 tttaccttgt cattaattaa ggaaatgaaa atgacattta tgaaaggact accccttttg
     3181 ttgttggttg ccagtctgtg cagccacgct gcactacaac ccgatcgcac tcgtattgtg
     3241 tttaacgcta atgataaagc taccagtctg cgggtggata atcgtagcga taaactcccc
     3301 tacctcgctt attcctggct cgaaaatgaa aaaggggaga aaagcgatga tcttctggtc
     3361 gccctgccgc cgatacaacg tctggaaccg aaagcaacaa cgcaggtacg gatagtaaag
     3421 caagcctcga ccacaaaatt accgggcgat cgcgaaacgc tatttttcta caatatgcgt
     3481 gaaattcctc ccgccccgga aaaaaacagc gaccatgccg ttctgcaagt cgccattcag
     3541 agtcgtatca aggtgttctg gcgaccggct gcattacgca agaaagcagg agaaaaggtc
     3601 gaactgcagt tacaggtcag ccagcagggc aaccaactga cactgaaaaa tcctaccgcg
     3661 tattatctga ccattgccta cctgggacga aatgaaaaag gcgttctccc cggcttcaaa
     3721 accgtgatgg ttgcgccttt tagtacggtc aacacaaaca tcggaaatta tagcggcagc
     3781 cagttttatc tcggttatat ggatgattac ggcgcgttac gcatgacgac gctcaactac
     3841 agcggacaat gccatttaca agccgtggag gcgaagaaat gagtgctggc aagggagtgt
     3901 tgctcgtcat ctgtctgtta tttctgccgt tgaagtctgc gcttgccctg aactgctact
     3961 ttggttcatc cggtggctcg gtagaaaaat cagaagcgat tcaacctttt gctgtaccag
     4021 gcaatgccaa actcggcgat aagatttggg aatctgacga tattaaaatt cccgtctatt
     4081 gtgacaacaa taccaacggc aattttgaaa gtgagcatgt ttatgcctgg gtaaacccct
     4141 acccgggggt gcaggatcgc tattatcaac tcggcgtaac ttacaatggc gttgattatg
     4201 acgccaacca gggaaaaagc cgcatcgaca ctaaccagtg tattgacagt aagaacattg
     4261 acatttatac ccctgagcag attatcgcga tgggatggca gaacaaaatt tgctcgggtg
     4321 atcccgctaa tattcacatg tcacgtacct ttctcgctcg catgcggtta tacgtcaaaa
     4381 tacgagaaat gccgccgcat gattatcaaa gtacgcttag cgactatatc gtcgtgcaat
     4441 ttgacggtgc cggtagcgtt aacgaagatc ctactgccca aaacctgaaa tatcatatta
     4501 ccggtctgga aaacattcgc gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa
     4561 cacaggtgat tgattttggt aaatttaatt tgctggatat tcgtcgccac acaatgtcga
     4621 aaacattcag tattaagacg acaaaatcac aaaatgacca atgcaccgac ggatttaaag
     4681 tcagttcctc cttttatacc gaagaaacgt tggttgaaga agataaagca ttgttgattg
     4741 gtaatggtct gaagctacgt ttgctggatg aaaacgcctc gccttatacc ttcaataaat
     4801 atgctgaata cgccgatttc accagcgaca tgttggtcta cgaaaaaacc tatacggctg
     4861 aactttcgtc cattgcaggt acccccatcg aggctgggcc cttcgataca gtggtgcttt
     4921 ttaaaattaa ctataactga tttgccatga tgccctttgc agccggaaag ggcatctctt
     4981 tcagaaaatt gctgattttc cccttttcct tgattgctgt tattttttcc tggcccggct
     5041 ttctttatgc tgtatcgata caccaaagag aaaataatga gggagcgaag gatgccagtt
     5101 ttgcagtggg gaatgttatg tgtgctatca cttctccttt ctattggttt cctcgcggta
     5161 catctcccgg cggcgctatt acttgggccg atgatcgccg ggatcatctt cagtatgcgc
     5221 ggaattactc tgcaacttcc ccgctccgct tttctcgccg cgcaggccat tcttggctgc
     5281 atgattgcgc aaaacctcac tggttcgatt ctcaccacgc tggccgccaa ctggccgatc
     5341 gtgcttgcta ttttactggt gacgctgctt tccagcgcca tcgtgggttg gttattggtg
     5401 cgctatagct cactgcctgg aaataccggt gcctggggtt cttctccagg cggcgcagcg
     5461 gcaatggtcg ctatggcgca agattacggc gcagatattc gcctggtggc gtttatgcaa
     5521 tatctgcgag tgctgttcgt cgcgggggcc gctgttctgg tcacccgcat gatgctgggt
     5581 gataacgctg aagcggttaa ccagcaaatc gtctggttcc cgccagtgag cattaatctc
     5641 ctgcttacga ttttgctcgc agtcgttgcc ggtacggcgg gatgcatgct gcgtcttcct
     5701 tccggcacga tgctcatccc gatgctggcg ggcgcagtgc tccagtctgg tcagctcatc
     5761 accatcgaac tcccggaatg gctgcttgcg atggcgtata tggcaattgg ctggcggatt
     5821 ggtcttggtt tcgataaaca aatcttactg cgggcactac gcccgctacc gcaaatcctg
     5881 ctgtcgattt ttgctctgtt ggctatttgt gcgggtatgg cgtgggggct gacccggttt
     5941 atgcatattg attttatgac tgcctacctc gccaccagcc ccggcgggct tgataccgtg
     6001 gcggtcatcg ccgcagggag caatgccgat atggcgctta ttatggcgat gcaaaccctg
     6061 cgcctgttca gtattttgct gacgggacct gccattgcac ggtttatttc aacctatgcg
     6121 ccgaagcggt cggcttagtg ctggcagcca gggcaccagt aaaacgggcg agatgacagc
     6181 gtggttttct caatgatgct gccacaacgt tcgcacggtt cgccatctcg atgaaaaacc
     6241 ttaaagcgaa acagcgcccc atgatgctta ttctcatcca cctgcccccg cgtagcgtaa
     6301 gaaaatcgag gaatctccag taacgcgtgt gcgagtgcat ccagttgcgc cgcattgaga
     6361 tctttcgctt tatgatttcc agtcaaccca acctgccaga ggatctccac ccgcaaataa
     6421 ttgccaagtc ctgccagaaa cgcctgctcg agcagtaatc ctgcaaactg acggttacga
     6481 aagcgcggcg acaatacccg ttctttcacc acctccggcg tcagattcgg atccagcaca
     6541 tcgggaccaa cgcgttgtaa aaacggatgc gtggtcagtt gttccggggt caacatctca
     6601 atatccgagg cgctataaag cagaatggtt ttgtcagccg tttgcagttt tacccgcaat
     6661 actcgcgtgg tctgcggctc ttcgccggta tcaaccacgc gccagacgcc gtaaagctga
     6721 ttatggctgt agagcgttaa ttcgttggaa aaatgagtta acaacgcctt accacgcgtt
     6781 tccacatggg taacgtgttg accgataagt tgtgattgat aagtttttaa ctgcgggaag
     6841 gcaaaccaga catcagttag tggtttgcct ttgatcgccg cctccaggtt atccgctgca
     6901 cggcggatct ccgggccttc aggcatggtg atatcctttt ttcattcttt attaagcggg
     6961 gttatgctgc gacaacaatc ccctttttgg caaatgtagc tcgtaatcgg cgggcgaacg
     7021 ccagagcgtg ctcgccgtcg ccatgcaggc agaccgtttg cgccgcgacc gttgcccatt
     7081 cgccggtgat acttttgact ctgccgtgtt gcaccatctc cagcgtttgc gccagcgcct
     7141 gttgttcgtt ttcaatcaac gcgcctggct gacttcgcgg caccagcgag ccgtcagcct
     7201 gataaccgcg atcggcaaac acttcctcgc gcgttgtcag gccatattgc ttgcctgcac
     7261 gaatcagctc gcttcctgcc agcccaacga gaatcaatgc cggatcgcaa gcgtataccg
     7321 ctctggcgat ggcgtctgcc agttgtgctt ctttcgccgc ctggttgtac aacatgccgt
     7381 gcggtttgac atgacgcatt acgccgcctt gcgcacgggc aatcgttgcc agcgcgccaa
     7441 tttgatacag cgtctgggcg taaacggttt ctggcggcaa ctgcatggcg ctgcgaccaa
     7501 aattttccct gtcgggaaaa ctcgggtgag cgccaattgc aacaccattt tttattgctt
     7561 cacgcacgca agcctgcatg gtttgcgcat cgcctgcatg aaatccacag gcaatattgg
     7621 cagaggaaac cagcgttaat agctctgcgt cgctggcgca gccttcgccc agatcggcgt
     7681 tcaggtcaat tttcattgtg cagccgccac gctaattgtt caaaataacg ttgctgatct
     7741 tgccgcgctt tcagtgcctc ttccagtgaa cactggacaa aatgaatcgg ctgaccgagc
     7801 ggaatttgcg caagatggta catatcagcc tcaatgatgc aggcaatacg cgggtaacca
     7861 ccggtggtct gtgcgtcgtt catcaacaca atcggctgcc cgttatgtgg cacctgcacc
     7921 acgcccggta acaaaccgtg agataacagt tcgcgatcgg tggtgcgttt taaaatttgc
     7981 ccctgtaagc gatagcccat gcggttactt tgcgagctaa gctgccaggg cgaacgccag
     8041 aatgcatcct gcgaggcgcg atcgaactca tgatattccg gccccggcaa ggcgcgaatg
     8101 cggttgcccc acagcagctg tttaacgccc tgcgcttcca taaaatcacg cttcgctttg
     8161 ccaatcggga gtcggtcacc atccttcagt aaacggcctt ccagcccgcc actccccact
     8221 ttgagatcgg tgctgcatga ccccattacc ggcggaacat caataccacc cgcgaccgcc
     8281 agataactgc gcatcccgtg ctgcgggcgt ttaagcgtta aacgctggcc tgctttcatc
     8341 ggcaatcgcc agccggtcca gacggcatta tcatccagcc gcgcttcgca accggcaccc
     8401 gtcagagcaa accacccatc agtttcgaac tcaacagtta actgaccgag cgtgatctcc
     8461 agcgcggggg cattggcgtc attacccacc agtaggttag caatgcgtaa cgcgggcata
     8521 tccagtgcgc cgcagtggct gatacccgac tggcgaaaac cgtgacgacc gccatcctgc
     8581 acagtggtat acatgcccgc acgaataatc ttcagcatac tccctccttc tgcggtacaa
     8641 agcgcacgct gtctcccgga cgtaataaga tgggttcgtc acgcgccgga tcaaacaggc
     8701 tgagtgaggt atgaccaatc aactgccagc cacccggcgt tgccagcgga taaacaccag
     8761 tctgcggccc gccgatcccg acagaacctg ccggaacgag taagcgcggt tcagcgcgcc
     8821 gtggcgtgtg tagttgttcc ggcaaactcc cgagatacgg gaagcccggt tgaaaaccta
     8881 aaaaccagac cacgtattcc acggaggagt gcaattcaac aacctgtttt tcgctcaacc
     8941 cgcaatgcgc cgcgaccacc gccaaatccg gtccgcctgc accaccgtaa accaccggaa
     9001 tttcaataaa gcgagactca ggttccaggg cctcgctctc ctcccaccag cgttgcaaac
     9061 gctctatggc atccagcgcc agcgactcag gattacgcaa aatcaccgta atattgttca
     9121 tgccgggaat ggcttcaacc acattcggca tatccaccag acgctgcgcc agtcgccaga
     9181 tccgtttctg gctagccagc gtcaccggcg gttccagttc cagcactacc gccgtttcac
     9241 ctatcagata acaacgcgct cgttgcactt tcgatccctc tgattattcg ttatgcagga
     9301 ttaggaatat caataaaggt cacatcaaga tcggtatttt cattcagcca ctcgctcaat
     9361 gcgcgaatac caccacgttc agtggcatgg tgacctgcag cataaaaatg caatccctgc
     9421 tcgcgggctg aatgaatggt ctgttcagaa acttcgccag taataaaagc atccacgcca
     9481 aaacgcgcgg cgctatcgat aaaactttgc ccgccgcccg tgcaccaggc gacgcgctgg
     9541 accacctcag gtccggtatc gccacaccat aacggcttac gtcccagacg cgcttcaatc
     9601 caggaggcta gttccagtcc cggcacaggc atggtcagct ctccccacgg caccaacggc
     9661 tcaatttcgc ccatgaccgt gatccccagt aacgccgcca gctgtgcgtt attgcccagc
     9721 tcaggatgtg cgtcaagcgg caaatgccag ccatacaggt tgatatcatt cgccagcaac
     9781 gtttttaaac ggttacgctt cattccgcga atgaccggag actctccttt ccagaagtag
     9841 ccatgatgca caatgaccgc atccgcgccc agacgcactg cctcatcgag caaagcctgg
     9901 ctggcggtga caccggtaac aattttttgc accgtctctt tgccttccac ctgcaaacca
     9961 ttcggcgcgt aatcactaat cgccgcgctg ttcagttttt cgttaatcag ttgttccagt
    10021 tcggtgtttt tcatcttcgc gccttccgta ttctgatagc tacaccgtta acatagcgtt
    10081 acctcgcctg ttcgtcgata gccaatcttt tgacatatta agaaacaaac ccgacgccaa
    10141 aagacgcaaa ttgtttatat catcacgctt cagctaatgt atatttatgc aatcagacag
    10201 catattgcat aaataatcag atctgtatca aagttttaac tttaaggaaa cacaagcacg
    10261 ccgcgcaact attacgtgac gttgccgtca attgtggaat tattatgaat aaacacgcat
    10321 cacaaccgcg cgctatttac tatgtcgttg cgctgcaaat ctgggaatat tttagctttt
    10381 acggcatgcg tgccctgctg attctctatc tcaccaatca actaaaatac aacgatactc
    10441 acgcctacga gttatttagc gcctactgtt cgctggtgta tgtcacgcca atcctcggtg
    10501 gctttttggc ggataaagtt ctcggcaatc gcatggcggt gatgctgggg gcgttgttga
    10561 tggcgatcgg tcatgtggtg ctgggtgcca gtgagatcca tccgtcattc ctctatctgt
    10621 ccctggcgat tatcgtttgt ggctatggtc tgtttaaatc caatgtcagt tgcctgctcg
    10681 gcgagctgta tgagccaacc gatccgcgtc gtgatggcgg tttctcgctg atgtatgcag
    10741 cgggtaacgt ggggtctatt atcgcaccta tcgcctgtgg ttacgcccag gaagagtaca
    10801 gctgggcgat gggctttggc ctggcggcgg tgggtatgat cgcgggtctg gtcattttct
    10861 tatgtggcaa tcgtcatttc acacataccc gcggcgttaa taaaaaagta ctgcgtgcaa
    10921 caaactttct cctgcctaac tggggatggc tgctggttct gctggtggca acgcctgcgc
    10981 tgattaccgt actgttctgg aaagagtggt cggtatacgc cttaattgtc gcgactatta
    11041 ttggcctggg tgtactggca aaaatttatc gcaaagcaga aaaccagaaa cagcgtaagg
    11101 agctggggct gattgtgacg ctcaccttct tcagtatgtt gttctgggcc ttcgcacaac
    11161 agggcggcag ctcgattagc ctttatatcg accgcttcgt taaccgcgat atgtttggtt
    11221 ataccgttcc gactgctatg ttccagtcga ttaatgcctt cgcagttatg ctgtgcggtg
    11281 tgttcctggc gtgggtggtt aaagaaagcg tcgcaggtaa tcgtaccgtg cgcatctggg
    11341 gaaaatttgc tcttggcctt ggcctgatga gcgccggatt ctgcattctg accttaagcg
    11401 cccgctggtc cgcaatgtat ggtcactctt ctctgccact gatggtatta ggcctggcgg
    11461 tgatgggctt tgcggaactg tttatcgacc cggttgccat gtcgcaaatt acgcgcattg
    11521 aaattccggg cgtgaccggc gtattaaccg gcatctacat gctgctttct ggcgcgatcg
    11581 cgaactatct ggcgggcgtg attgccgatc agacatcgca ggcttcgttt gatgcttccg
    11641 gggcgatcaa ctactccatc aatgcatata ttgaagtatt tgatcaaatt acctggggcg
    11701 cactggcgtg tgtaggactg gtactgatga tttggctgta tcaggcgctg aaattcagaa
    11761 accgcgcgct ggcgctggag tcataatagg ccagcaccat acctgataaa acgctcgctg
    11821 cgttttatca ggctcctttc ctcgcttctt cgtacgctgc cagcgttcgc agccttgcct
    11881 ctttgtgatc aactatcggt tgcggataat ccagcatcac acctgctttc tgcgcccact
    11941 tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac catcggcgga
    12001 taaactcacc ttcgcgatca aatttctcgc cctgggttgt cgggttgaaa atacgaaaat
    12061 acggcgctgc atcagtaccc gtagacgccg cccactgcca gccaccgtta ttggctgcca
    12121 aatcaccatc aatcagctgc gacatgaaat atcgctcgcc ttcgcgccag tcgatcaaca
    12181 aatctttcac cagaaaactg gctgaaatca tccgtagccg gttatgcatc cagccagtgc
    12241 tgttaagctg acgcatggcg gcatcgacaa tcgggtatcc cgttttgcct tcctgccagg
    12301 cctgtaaatg tgcgggattg ctctgccact gcacacgatc cgtccaggca ataaacggac
    12361 aatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg cgccagatca
    12421 gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt tccgccaaca
    12481 agcgatgcaa acactggcga ggcgataacc cgcccgttgc caggctggcg gacaaacgac
    12541 tggtgccttc cactgccgga aaatctcgtt gttgctcata ttctccggca ccgttctggc
    12601 aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc gcagtatcga
    12661 aagactgacg aggataattc agcgtaatgg atggcgaggg ctctatcgat ccgctactac
    12721 gaacttttgg cgcagcgacg cactccggca tcccttcccg cagccgtttc agccaggcat
    12781 tcttaaaagg cgtaaagact ttgtacatct cgtgattacc ggtcttcacc gcgccaggcg
    12841 gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct ctttcaactt
    12901 ccacatcccg cgcccgctca ttcacttcat actgatagtt ataaaacagg tgggtaacgc
    12961 tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca tccacttcac
    13021 ggaacaataa aggaatacct ttttccgcaa gcgctatttg tagcccattc agttgagcat
    13081 tgataagttc agcctgacgc ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga
    13141 tatacaacgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt gcgagattat
    13201 cgtgcagacg taaatcctgg cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg
    13261 gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc
    13321 aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg gttgctcgcc
    13381 acggcgataa ctgtcgataa gcgccgccaa ctgctggcgt tctgtggaat caatatgcgg
    13441 gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt tggcgggatg
    13501 agaaagcaaa acaattacgc gttggtagta ctggttgtaa aaatcatcca gattttgcca
    13561 ctggtgaata tcagccacga acgggccaag tttgcggtac tccggctgag aatgcgccag
    13621 aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc
    13681 tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt catcaaatcg
    13741 ttgctgattc attactgctc ctgaacggtt tacaggagta agtgtagacg ttgagaaatg
    13801 atatttgtga agttaatcct gttgtagcag gacaaattta cgaaagcctg ctccccgcaa
    13861 ggactgacgt caggtagttt ctaaccatag ctgccctttc tattcaaacg tgaaatcaat
    13921 aagctcatca gggacatcgc aaacaatatg aagacgggtt tcttctctgc cgtgactctt
    13981 ttcactaatt gcgtaactgt catgcttggg attatttaat tctttcagcg gaaatttttc
    14041 ctcgaaggct ttatttagcc gcccctggtt tccttttaca gcgaataaat aatcacctcc
    14101 ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcgg ttgtgatgat
    14161 ttttccttta atatccagca tgttaagaag ttctgggata gctgtgatct cattggattt
    14221 cttatccgtc ttgatctgcc cgatgaccag actgtgcatt gttgagaacg cactaatgac
    14281 atgaatcgct cccctgcggc gactcttgtc ataagagtgc cggagcgttt ttccatcaat
    14341 tgcaatgacg tctttatcat ttgatgaatg gcaatcacgc atccagttaa taaagcactc
    14401 gtgaaatttt gcaggactga tacaggatac aactctggca atagtatcgt gaacaggaat
    14461 a
//
LOCUS       DAZFKM010000129        14283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFKM010000129 DAZFKM010000000
VERSION     DAZFKM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..278
                     /locus_tag="ACHKAY_003970"
     CDS             <1..278
                     /locus_tag="ACHKAY_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO5702557.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
     gene            278..5548
                     /gene="traI"
                     /locus_tag="ACHKAY_003971"
     CDS             278..5548
                     /gene="traI"
                     /locus_tag="ACHKAY_003971"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO5702558.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLREFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5568..6314
                     /gene="traX"
                     /locus_tag="ACHKAY_003972"
     CDS             5568..6314
                     /gene="traX"
                     /locus_tag="ACHKAY_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO5702559.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHANIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6369..6929
                     /locus_tag="ACHKAY_003973"
     CDS             6369..6929
                     /locus_tag="ACHKAY_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO5702560.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPQLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            7048..7260
                     /locus_tag="ACHKAY_003974"
     CDS             7048..7260
                     /locus_tag="ACHKAY_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO5702561.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            7547..8224
                     /locus_tag="ACHKAY_003975"
     CDS             7547..8224
                     /locus_tag="ACHKAY_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFO5702562.1"
                     /translation="MEENNTSTDKNTLTALIDYENCGSLKNISLEQYGELIIFVGPQQ
                     NVVVLPADSFPEGARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDK
                     GYDGVIRTLKEAGITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQ
                     QIAGGGAGNMPVSVETFNNYLKSHMRDEWNKTRAVAIRNELVRRGVTEVREKKIIWKK
                     AKKENKA"
     gene            8890..9081
                     /locus_tag="ACHKAY_003976"
     CDS             8890..9081
                     /locus_tag="ACHKAY_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702563.1"
                     /translation="MNGFRNSSRNGQVWRYQSAGSRAVILEAAEAWRRAAGVAPRTDW
                     QQFARKRAEQCHRRCRGRV"
     gene            9325..9786
                     /locus_tag="ACHKAY_003977"
     CDS             9325..9786
                     /locus_tag="ACHKAY_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO5702564.1"
                     /translation="MMKYIPLVLFIFSWPVLSEDIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVFQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            9832..10041
                     /gene="hha"
                     /locus_tag="ACHKAY_003978"
     CDS             9832..10041
                     /gene="hha"
                     /locus_tag="ACHKAY_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5702565.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            10079..10669
                     /locus_tag="ACHKAY_003979"
     CDS             10079..10669
                     /locus_tag="ACHKAY_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5702566.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10909..11166
                     /locus_tag="ACHKAY_003980"
     CDS             10909..11166
                     /locus_tag="ACHKAY_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO5702567.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            complement(11202..11354)
                     /locus_tag="ACHKAY_003981"
     CDS             complement(11202..11354)
                     /locus_tag="ACHKAY_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO5702568.1"
                     /translation="MAPVIFSQSPNLTKIIGVFTFLAPVNPHQNQFPATLRRGQPQNS
                     LKRSAI"
     gene            11403..11477
                     /gene="tap"
                     /locus_tag="ACHKAY_003982"
     CDS             11403..11477
                     /gene="tap"
                     /locus_tag="ACHKAY_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO5702569.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            11470..12327
                     /gene="repA"
                     /locus_tag="ACHKAY_003983"
     CDS             11470..12327
                     /gene="repA"
                     /locus_tag="ACHKAY_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO5702570.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            13243..13512
                     /gene="yacA"
                     /locus_tag="ACHKAY_003984"
     CDS             13243..13512
                     /gene="yacA"
                     /locus_tag="ACHKAY_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFO5702571.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            13512..13622
                     /gene="yacB"
                     /locus_tag="ACHKAY_003985"
                     /pseudo
     CDS             13512..13622
                     /gene="yacB"
                     /locus_tag="ACHKAY_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            13721..>14283
                     /locus_tag="ACHKAY_003986"
     CDS             13721..>14283
                     /locus_tag="ACHKAY_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702572.1"
                     /translation="MTIESFFIGTRRSDKRYGPQSKDMQVSEFISLISPKNAPHKVVL
                     PDFTGLAIRLDAQIRNQFHQLKEDEHFLRYRQLSERWYQAGSISDRNNRSKRFEKIMD
                     DSLDFLLYSQDVMPNINPDDLQWHDYEKASSKGKMYCVALLFHVIARAAYEPESVGKD
                     PTLPEYCRWMKNWIEKTLGHDFLDRMM"
BASE COUNT         3625 a         3345 c         4234 g         3079 t
ORIGIN      
        1 tgtcttctgt catcaatgat aagaagtcag atgcaggtgt gagtgttccg gcagggggga
       61 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
      121 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcaa caggaaaatc
      181 atccggacat ccagcagcat atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg
      241 agcgcgggga ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa
      301 atcggcaggc agtgccggaa actattacac cgacaaggat aattactatg tgctgggcag
      361 catgggagaa cgctgggctg gtcagggggc tgaacagctt gggctgcagg gcagcgtcga
      421 taaggatgtt tttacccgtc ttctggaggg caggctgccg gacggggcgg atttaagccg
      481 catgcaggat ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa
      541 aagtgtctcc atgatggcca tgctcggggg ggataaacgt cttattgatg cacataacca
      601 ggccgtggat tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga
      661 cggacagtca gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accatgacac
      721 cagtcgcgat caggacccac agttacacac gcatgtagtg gtggctaatg tcacgcagca
      781 taacggcgag tggaagacac tgagcagtga caaagtgggg aaaaccggat tttccgagaa
      841 cgtgctagcg aaccggattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt
      901 tgaggcgctg ggttatgaaa ccgaagtggt cggtaagcac ggtatgtggg agatgccggg
      961 cgtaccggtg gaggcctttt ccagtcgttc acaggctatc agggaggccg tcggtgaaga
     1021 cgcctcgctg aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt
     1081 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     1141 ccgggcatat cgtgacgcgg cggatcagcg tgcggagatc cgcacgcagg cgcccgggcc
     1201 tgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggcgattg ccggattaag
     1261 tgaacgaaaa gtgcagttca cgtatacgga cgtgctggcc aggacggtcg gcatactgcc
     1321 gccggaaaat ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga
     1381 gcagcttatc cccctcgacc gtgagaaggg gctgttcaca tcaggaattc atgtgctcga
     1441 tgagctgtcc gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca
     1501 tccggagaaa agtgtccccc ggacggctgg ttacagcgat gcggtcagtg ttctggcaca
     1561 ggaccgtccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     1621 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc
     1681 tgaccgtcgc tcgcagatga acctgaagca ggatgaacgg ctgtccggtg agctgataac
     1741 cggacgtcgt cagctgcagg agggcatggt cttcacgccg ggcagtactg ttatcgtcga
     1801 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca
     1861 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat
     1921 ggccatgaag gatgccgggg tgaacacgta tcgctggcag ggcggagaac agcgaccggc
     1981 aaccatcatc agtgaaccgg accggaatgt ccgctatgac cggctggccg gagattttgc
     2041 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggggtac gggaacaggc
     2101 catactgaca caggccattc gcagtgagct gaaaacacag ggcgtgctcg gacacccgga
     2161 ggtgaccatg accgccctgt caccggtctg gctggacagc cggagccgtt atctgcggga
     2221 tatgtaccga ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     2281 ctatgttatc gaccgggtga cggcgcagag tcacagcctg accctgcggg atgcgcaggg
     2341 cgaaacgcag gtggtgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga
     2401 aaaaatgccg gtggcagacg gcgagcgact gagggtgaca gggaaaattc ccggactccg
     2461 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     2521 tgtgccgggg cgggctgaac cggccaccct gcctgtggct gattcaccgt tcacggcact
     2581 gaagctggag aacggctggg tggaaacgcc cgggcattcc gtcagtgaca gtgcgacggt
     2641 gtttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     2701 cggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaacttgc
     2761 ccgccatccc tcctttacgg tggtttctga gcagataaag gcgcgggccg gtgagacatc
     2821 gctggaaacc gctatcagtc tgcagaaaac cgggctgcac acgccggcac agcaggccat
     2881 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     2941 gacagaggcg aagtcgtttg ctgcagaagg aaccggtttt actgaactgg gaggggaaat
     3001 caatgcgcag ataaaacgcg gtgacttact gtatgtggat gtggcaaaag gctatggcac
     3061 aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaaa agtattcttc gccatattct
     3121 cgaaggtaag gaggcggtca cgccgctgat ggagagagta cccggcgaac tcatggagac
     3181 gttaacgtcg ggacagcgtg ccgccacccg tatgatactg gaaacgtccg accgtttcac
     3241 ggtggtacag ggttatgccg gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     3301 cgtgaacatg ctgccggcga gtgagcgtcc ccgagttgtg gggctggggc ccacgcaccg
     3361 tgcggtcggt gagatgcgca gcgccggcgt ggatgcacag acgctggcgt cctttctgca
     3421 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     3481 gctcgatgag agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     3541 ggccggtggc ggtcgtgccg tggccagtgg tgacacggac cagctgcagg ccatcgcgcc
     3601 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggtca tcatgaagga
     3661 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     3721 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggttcccc gtcaggaggg
     3781 cgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     3841 agaagcgcag cagaaggcga tgctgaaagg tgagactttt ccggatgtcc ccatgacact
     3901 gtatgaagcc attgtccgcg actataccgg caggacaccg gaagcacggg agcagacgct
     3961 gattgtcacg cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg
     4021 ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg cctgtcctga acacagcgaa
     4081 tatacgtgac ggggagctgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     4141 cctggtggat agtgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac
     4201 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     4261 tgtcacactg tacacaccag acaaaatccg ggtggggacc ggcgaccgga tgcgcttcac
     4321 gaagagtgac cgggagcgcg gttatgtggc caacagcgtc tggacggtga cagcggtttc
     4381 cggtgacagt gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccggccagga
     4441 gcgggcagag caacatattg acctggccta tgccatcacc gcccacggtg cgcagggtgc
     4501 gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tgatggccgg
     4561 ctttgagtca gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga
     4621 taaccgtcag ggctggacgg atgccatcaa caatgccgta cagaaaggaa cggcccacga
     4681 tgtgtttgaa ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc
     4741 acgggaactg cgggacgtgg cggcaggccg tgccgttctc cgtcaggcag ggctggccgg
     4801 gggagacagt cctgcacggt ttattgctcc gggacgtaaa tatccgcagc cgtatgtggc
     4861 actgccggcg tttgaccgta acggcaggtc cgccggtatc tggctgaacc cactgaccac
     4921 ggatgacgga aacgggctgc gggaattcag cggtgaagga cgggtgaaag gcagcgggga
     4981 tgcgcagttc gtggccctgc agggcagccg taacggagag agcctgctgg ctgataatat
     5041 gcaggatggt gtccggattg cccgtgataa tcctgacagc ggtgtggtgg taagaattgc
     5101 cggtgaaggt cgtccgtgga atcccggtgc catcaccggt ggtcgcgtgt ggggggatat
     5161 cccggacagc agcgtccagc cgggagccgg aaatggcgaa ccggtcacgg cagaggtgct
     5221 ggcacagcgg caggctgaag aggccatccg ccgtgaaacg gaacgccgcg cagatgaaat
     5281 tgtcagaaaa atggcagaga acaaacctga cctgccggat ggtaaaacag agcaggctgt
     5341 cagggaaatt gccgggcagg agcgtgaccg ggctgacata actgaacggg aagccgcgct
     5401 gccggagagt gtgctgcgtg aatcacaacg ggagcaggaa gcggtcaggg aggttgcccg
     5461 ggaaaatctg ctgcaggagc gtctgcagca gatggagcgg gatatggttc gtgacctgca
     5521 gaaagagaaa accctgggcg gagactgata caggaagaca aacactgatg acaacggata
     5581 acacgaacac gacacgtaac gattcactgg ctgcacggac cgatacctgg ttgcagtcat
     5641 ttctggtctg gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgctgatgg
     5701 tactggacca catcaatctg atattccagc tgaagcagga atggatgttt ctggccgggc
     5761 gaggagcctt tccgctgttt gccctggtgt gggggctgaa tctgtcccgt catgcgaata
     5821 tccggcaacc ggccattaac cggctgtggg gatgggggat tattgcccag ttcgcatatt
     5881 atctggccgg ctttccctgg tatgagggga atatcctgtt tgcctttgca gtggcggcac
     5941 aggtgctgac gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc attcttctga
     6001 tggccctgtg ggggcctttg tccggcacca gttacggcat tgccgggctg ctgatgctgg
     6061 cagtcagcca ccggctgtac cgggcagaag acagagcgga acgtctggcg ctggtcgcct
     6121 gcctgctggc tgttattccg gcgcttaacc ttgccaccag tgatgcagcg gcggtggctg
     6181 gtcttgtgat gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc
     6241 gtttctggcc cggggatttt ttcccggtat tctacgcctg tcatctggct gtgctgggcg
     6301 ttctggcgct gtgacgggtg tggtatcttt ggccgcaaga ggatgattcg tcagaggcag
     6361 aacacagcat gacagagcag aagcgaccgg tactgacact gaagcggaaa acggaagggg
     6421 agacgcctgt ccgcagccgg aaaaccatca tcaatgtcac cacgccacca aagtggaagg
     6481 tgaaaaagca gaagctggcc gagaaagcag cccgggaagc agagctggcg gcaaaaaaag
     6541 cgcaggccag acaggcactg tccatttatc tgaacctgcc cacgctggat gaggccgtga
     6601 ataccctgaa gccctggtgg ccgggattgt ttgacggtga cacaccccag cttctggcct
     6661 gcggtatccg ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata
     6721 aaaaactgcg cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga
     6781 aagccggtgc ctgccggtat gacacggaag ggtatgtgac ggagcatatt tctcaggagg
     6841 aggaagtgta tgcggcagcg cgtctggata aaatccgccg ccagaacagg ataaaggcag
     6901 agcttcaggc cgtgcttgat gagaaataaa aaaaagcctc catcagagag gaggcaggga
     6961 aataaggctt aaaaggaaac caaaggaagc caattcagta gattatttaa caccataata
     7021 ctgacgggtt aaagaggagt gataaagatg aatggattca gaaacagttc acggaacggt
     7081 caggtctggc gctggcagcg tgccggaagc cgggccgtta ttctggaggt cagtggacgc
     7141 tggatggaag cggcagaagc atggcgacgg gctgccggtg ttgcgccccg gacagactgg
     7201 cagcagtttg cccgaaaaag ggctgaacac tgccaccggc gcagccgggg aagggtgtaa
     7261 cgaaaaaagc agccttcatt actccaggga ttcagaaggc tgaagtatgc atgagatttt
     7321 tcttttttat atctggcctg gcagtatgac cagtcagcat tcattatcac aggattattg
     7381 ctgtattaag taataacgtg ctgttttgtg attgatttca taaaacaaga cgtcgtttaa
     7441 tatgcgcctg aatattcacg agggaagtct ttctttttat atggtgttcc cggaaataaa
     7501 atattttgtg ttattgctca cccggttatg aataaaaggt aaaaacatgg aagaaaataa
     7561 tacgagtaca gataaaaaca ccctgacagc ccttattgat tatgaaaact gtggcagtct
     7621 gaaaaatatt tcccttgagc agtatgggga actgattatt tttgtcgggc cacagcagaa
     7681 cgtcgttgtt cttcctgcag actcttttcc tgaaggtgcg agaatcacta tcaggcaggt
     7741 gagtggtgtg tccaggaata atgtcgattt tcatcttgtt ctggagctgg ggcggataag
     7801 ctgttgtgca gcagggaaag ataaaacata ccacatcatt tcatcggata agggatatga
     7861 cggtgttatc aggacgctga aagaagccgg tattacctgc atccgtcgtg atgtaccgga
     7921 aagtattgct gctgccggtg agtctggtgc agacaaaagt aactgcctga aacaaccttc
     7981 tgttcctgaa agtgatatcg tcagccgttg ggcaggaaag ctgcagcaga tagccggcgg
     8041 cggagccggt aatatgcctg tgtccgtcga aacatttaat aattatttaa agtcacacat
     8101 gcgtgacgaa tggaacaaaa cccgggcagt ggcgataagg aatgaactgg ttcgtcgggg
     8161 ggtcacagaa gtcagggaga aaaagataat ctggaaaaag gcgaaaaagg agaataaagc
     8221 atgaggtatc gcagctgtac tcagataaaa acagcccccg ttgcggttgc ggagcgaagg
     8281 gggctgaaaa ggggaattaa ggatgattgt tattatggtg aaccagaagg ttatccctgt
     8341 ctgtaagctg aatcggacta aacgacataa tacctgtaac aatacctgta acatattttc
     8401 ctggcaggac aagcaggaac aataaaatgt gtgatttaat tcaaaaaatg tgcgcggcaa
     8461 agaaaaaccg gggaattgct tccccggtcc ggtccattta gccagttccc tgagcaggat
     8521 ctgatatgag cattattcac tttgccagac atggtgaaaa aagtgaatat atggaggaga
     8581 aaaacatcgc ctgaatcaca gattcgggtg ctcagcgcgt aagaagacgg gaaagaaaag
     8641 cgcctttccg tttcagcatc gggaataatc actgaaacat cagcagtata tgtggtgtgt
     8701 atgaatatcc cggcagcatt aattcttctg tgaggaaatg cattgataag aaacagaata
     8761 agtcggtggc aaggaaaatg aatttattaa ttcagcatcc gtttatcaga taaaaaaggg
     8821 aaagagtatg gtgaaactga ttatttttat tatcacaata atactgacaa ctaaaggggg
     8881 gacaggaaaa tgaatggatt cagaaacagt tctcggaacg gtcaggtctg gcgttaccag
     8941 agtgccggaa gccgggccgt tattctggaa gcggcagaag catggcgacg ggctgccggt
     9001 gttgcacccc ggacagactg gcagcagttt gcccgaaaaa gggctgaaca atgccaccgg
     9061 cgctgccggg gaagggtgta acgaaaaagc agccttcatt actccaggga ttcagaaggc
     9121 tgaataatgc atgagatttg ctttttttaa tatccggtcg ggcagtatga ccggacagtt
     9181 ctgagtagca cagagttttt tccgtattaa gtcgtgattt tatattttgt gatttatttc
     9241 acaaaataag gtgttattca ttgtgtgctg caatgttcag gatgcctgaa atacaggtgc
     9301 catttatctg atatggagaa taacataatg aaatatattc cactggtatt atttatcttt
     9361 tcatggccgg tattgtctga agatattcat ggacgggttg ttcgtgtact ggacggtgat
     9421 accatcgagg tcatggactc acggaaggcg gtgcggatca ggctggtaaa tattgatgca
     9481 ccggaaaaga aacaggatta tggccggtgg tcaacggaca tgatgaaatc actggttgca
     9541 ggaaaaacgg ttaccgtcac atattttcag cgagatcgtt atggtcgtat tctcgggcag
     9601 gtttatgcac cagatggaat gaatgttaat cagtttatgg tccgggccgg ggctgcctgg
     9661 gtatatgagc agtacaacac agatcccgtg ctacctgtct ttcagaacga agccagacaa
     9721 caaaaacgtg ggctctggtc agatgctgat ccggttcccc cctggatatg gagacaccgt
     9781 aaataaagcg ctggcaatct tatcattcat ctgttctcag cgggaataaa aatggcaaaa
     9841 acaaaacagg aatggctgta tcagttacgg cgatgttcat cagtcaatac gctggaaaaa
     9901 attattcata aaaacagaga cagtttatta aacagtgaac gtgaatcatt taattcagca
     9961 gcagaccacc ggttagcgga attaatcacc ggaaagttat atgacaggat accaaaagag
    10021 atatggaaat atgtaaggta ataattcaga ataattaatt accaggagca gataaacaat
    10081 gaagctgatt atttttattc tcattgttct gattattgca gcgttactta tccggattat
    10141 cctcagaagt gtaaatcagc attctccttt gctgatgcag ttgcatgcgg caggtatacg
    10201 gaccggtgat gcagagcgaa tactgtccgg cggtgaatac tggcaacgtc agaagacgct
    10261 gctgacagaa agggaagtca gttttatgaa aggactgttc agaattgtgg atatgaagcg
    10321 gtggtatctg tgtccgcagg tacgggtcgc ggatatcgtc cagctgaacg ggaatatccg
    10381 gccacgatca cgccagtggt ggcagttatt caggatggtg tctcagtggc atgttgatgt
    10441 ggttatcgtt gagcggcgtt cgttcagtat tgttgcagca gtagagctgg atgatgccag
    10501 ccatttacga ccggaacgca gacgccggga tattcttctg gaagaggttc tgaggcaggc
    10561 tggtattccg ttgctcagaa gccacgatgc cagaaaactg ctgcagatga ccggagaatg
    10621 gctgaataca acaggggctg atcagcagtc cccggaacat cgtagctgac gccttcgcgt
    10681 tgctcagttg tccaaccccg gaaacgggaa aaagcaagtt ttccccgctc ccggcgtttc
    10741 cataactgaa aaccatacta tttcacagtt taaatcacat taaacgacag taatccccgt
    10801 tgatttgtgc gccaacacag atcttcgtca caattctcaa gtcgctgatt tcaaaaaact
    10861 gtagtatcct ctgcgaaacg atccctgtct gagaagagag gaggcgagat gtcgcagaca
    10921 gtaaatgcag agacttcctc atcgggaaaa aaacgacctt acagaagggg taatcctgtt
    10981 cctgcgagag aacgacaaaa agcgtctctt gcaagaagga gtgccactca taaagcgttt
    11041 catgccgtca ttcagcttcg actgaaagaa aagttaagtg agcttgctga tgaagacggg
    11101 atcactcagg cacagatgct tgagtggctg atagagtcag aggttaagcg cagaaaatct
    11161 ttgtgaatat ttgcgtttct tgctgtttca gtgatgagat gttagattgc tgatcgtttt
    11221 aaggaatttt gtggctggcc acgccgtaag gtggcaggga actggttctg atgtggattt
    11281 acaggagcca gaaaagtgaa aaccccgata atcttcgtca agtttggcga ctgagagaag
    11341 attaccgggg ccatctaaaa accgtatagc caacaattca gctatgcggg gagtatagtt
    11401 atatgcccgg aaaagttcaa gacttctttc tgtgctcact ccttctgcgc attgtaagtg
    11461 caggatggtg tgactgatct tcaacaaacg tattaccgcc aggtaaagaa cccgaatccg
    11521 gtgtttacac cccgtaaagg tgccggaacg ctgaagttct gcgaaaaact gatggaaaag
    11581 gcggtgggct tcacttcccg ttttgatttc gccattcatg tggcgcatgc ccgttcgcgt
    11641 ggtctgcgtc gacgcatgcc accggtgctg cgtcgacggg ctattgatgc gctcctgcag
    11701 gggctgtgtt tccactatga cccgctggcc aaccgcgtcc agtgctccat caccacgctg
    11761 gccattgagt gcggactggc gacggagtct gctgccggaa aactctccat cacccgtgcc
    11821 acccgtgccc tgacgttcct gtcagagctg gggttgatta cctaccagac ggaatatgac
    11881 ccgcttatcg ggtgctacat tccgaccgac atcacgttca cactggctct gtttgctgcc
    11941 cttgatgtgt ctgaggatgc agtggcagct gcgcgccgca gtcgtgttga atgggaaaac
    12001 agacagcgca aaaagcaggg gctggatacc ctgggtatgg atgagctgat agcgaaagcc
    12061 tggcgttttg tgcgtgagcg tttccgcagt taccagacag agcttaagtc ccgtggaata
    12121 aaacgtgccc gtgcgcgtcg tgatgcgaac agggaacgtc aggatatcgt caccctggtg
    12181 aaacggcagc tgacgcgtga aatctcggaa gggcgcttca ctgctaatgg tgaggcggta
    12241 aaacgcgaag tggagcgtcg tgtgaaggag cgcatgattc tgtcacgtaa ccgtaattac
    12301 agccggctgg ccacagcttc tccctgaaag tgatctcctc agaataatcc ggcccacgcc
    12361 ggaggcattc gcacgcctga agcccgtcag tgcacaaaaa atcagcacca catacaaaaa
    12421 acaacctcac catccacctt ctggtgcatc cggttccccc tgtttttaat acaaaacacg
    12481 cctcacagac gggtaattgt gcttatccac atttaactgc aagggacttc cccataaggt
    12541 tacaaccgtt catgtcataa agcgccagcc gccagtctta cagggtgcaa tgtatctttt
    12601 aaacacctgt ttatatctcc tttaaactac ttaattacat tcatttaaaa agaaaaccta
    12661 ttcactgcct gtcctgtgga cagacagata tgcacctccc accgcaagcg gcgggccccg
    12721 accggagcca ctttagttac aacactcaga cacaaccacc agaaaaaccc ctgtcctgcg
    12781 cagaaccgaa acaacaaagc ccctccctca taactgaaaa gcggccccgc cccggcccaa
    12841 agggccggaa cagagtcgct tttaattatg aatgttgtaa ctatactccg tcaccgctgt
    12901 cagtcttctc gctggaagta ttgagtacac gctcgtaagc ggccctgacg gcccgctaac
    12961 gcggagatac gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacaga
    13021 agctctatca tggctgaaaa cgggtatggc ttagcaggac tggggatggg taaggtgaaa
    13081 tctatcaatc agtaccggct tacgccgggc ttcggcggtt ttactccggt atcacatgta
    13141 accacagcgt gccgccttcc atgccgctga cgcggcatat tctggtgacg atatctgagt
    13201 cgttatatac tgtgtatata cgtagtaatg acgaggtgat aaatggcaca ggttaatatg
    13261 agtttaagaa tcgacgctga actgaaggat gcttttatgg ctgctgcaaa aagcatggac
    13321 cgtaatggct ctcagttaat ccgggatttt atgcgtcaga ccgttgaacg gcagcataat
    13381 acctggttcc gtgaccaggt tgaggcagga cgtcagcaac tcgagcacgg cgatgtactt
    13441 ccccatgaca tggtcgaatc ctctgctgca gcatggcgag atgaaatgag caggaaggtt
    13501 gccggtaaat gatggagata ttctggacca tgctggccag ccaggacagg aagcgcattc
    13561 gtgaatacat tgcggagcag aacctgatag cagccattga actggacgaa cggattggtt
    13621 atttcggacg aacgcaccgg cttacgccgg ttttacatct gtactacatg taacctcata
    13681 cacacataaa tgagtaacat actgattaat atggtaaatc atgacgattg aaagcttttt
    13741 tattggcacc cgccgctctg ataaacgtta cgggcctcag agcaaggata tgcaggtttc
    13801 tgaattcatt tctctgattt ctcccaaaaa tgcccctcat aaggttgtgt tacctgattt
    13861 tactggtctg gcaattcggc ttgatgcaca aatcaggaac cagtttcatc aactgaaaga
    13921 ggatgagcac ttccttcgat atcggcagtt gtcggaaagg tggtatcagg cgggctctat
    13981 tagtgacaga aacaaccgca gtaagcgttt tgagaagatc atggatgact cacttgattt
    14041 tttgctttac agccaagacg tcatgcctaa catcaatcca gatgatctcc aatggcatga
    14101 ttacgaaaag gctagcagta agggtaaaat gtactgtgta gcgcttctgt tccatgtcat
    14161 tgcacgggct gcatatgaac ccgaatctgt gggtaaagat ccgacacttc cagaatattg
    14221 ccggtggatg aaaaattgga tagagaaaac attaggtcat gattttttag atagaatgat
    14281 gat
//
LOCUS       DAZFKM010000130        14146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFKM010000130 DAZFKM010000000
VERSION     DAZFKM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(52..270)
                     /gene="ydfC"
                     /locus_tag="ACHKAY_003987"
     CDS             complement(52..270)
                     /gene="ydfC"
                     /locus_tag="ACHKAY_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5702573.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(342..641)
                     /locus_tag="ACHKAY_003988"
     CDS             complement(342..641)
                     /locus_tag="ACHKAY_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702574.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            977..1279
                     /locus_tag="ACHKAY_003989"
     CDS             977..1279
                     /locus_tag="ACHKAY_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5702575.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            1282..1641
                     /locus_tag="ACHKAY_003990"
     CDS             1282..1641
                     /locus_tag="ACHKAY_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5702576.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(1688..2080)
                     /locus_tag="ACHKAY_003991"
     CDS             complement(1688..2080)
                     /locus_tag="ACHKAY_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5702577.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            2207..2467
                     /locus_tag="ACHKAY_003992"
     CDS             2207..2467
                     /locus_tag="ACHKAY_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5702578.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            2464..2901
                     /locus_tag="ACHKAY_003993"
     CDS             2464..2901
                     /locus_tag="ACHKAY_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5702579.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2988..3998
                     /locus_tag="ACHKAY_003994"
     CDS             2988..3998
                     /locus_tag="ACHKAY_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO5702580.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            3991..4452
                     /locus_tag="ACHKAY_003995"
     CDS             3991..4452
                     /locus_tag="ACHKAY_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5702581.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            4486..5211
                     /locus_tag="ACHKAY_003996"
     CDS             4486..5211
                     /locus_tag="ACHKAY_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO5702582.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPKAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            5227..5619
                     /locus_tag="ACHKAY_003997"
     CDS             5227..5619
                     /locus_tag="ACHKAY_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5702583.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5616..5912
                     /locus_tag="ACHKAY_003998"
     CDS             5616..5912
                     /locus_tag="ACHKAY_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO5702584.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAR"
     gene            5909..6370
                     /locus_tag="ACHKAY_003999"
     CDS             5909..6370
                     /locus_tag="ACHKAY_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO5702585.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            6348..6704
                     /locus_tag="ACHKAY_004000"
     CDS             6348..6704
                     /locus_tag="ACHKAY_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702586.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            6755..6967
                     /locus_tag="ACHKAY_004001"
     CDS             6755..6967
                     /locus_tag="ACHKAY_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702587.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GNAPNYCWWEVDPVDVANEAMSYHND"
     gene            7001..7183
                     /locus_tag="ACHKAY_004002"
     CDS             7001..7183
                     /locus_tag="ACHKAY_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702588.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            7176..7352
                     /locus_tag="ACHKAY_004003"
     CDS             7176..7352
                     /locus_tag="ACHKAY_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702589.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            7349..7846
                     /locus_tag="ACHKAY_004004"
                     /pseudo
     CDS             7349..7846
                     /locus_tag="ACHKAY_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            8072..8290
                     /locus_tag="ACHKAY_004005"
     CDS             8072..8290
                     /locus_tag="ACHKAY_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5702590.1"
                     /translation="MTTLFRKEYPRKSRATEFLFLIIFIVLMIPIPPLLLVWFIVKII
                     EPVIELYNDVVWASFSTLHNKINPYKES"
     gene            8292..8657
                     /locus_tag="ACHKAY_004006"
     CDS             8292..8657
                     /locus_tag="ACHKAY_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO5702591.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            8654..8998
                     /locus_tag="ACHKAY_004007"
     CDS             8654..8998
                     /locus_tag="ACHKAY_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702592.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(9203..9502)
                     /locus_tag="ACHKAY_004008"
     CDS             complement(9203..9502)
                     /locus_tag="ACHKAY_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5702593.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(9508..9765)
                     /locus_tag="ACHKAY_004009"
     CDS             complement(9508..9765)
                     /locus_tag="ACHKAY_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5702594.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            9901..10173
                     /locus_tag="ACHKAY_004010"
     CDS             9901..10173
                     /locus_tag="ACHKAY_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702595.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            10175..11221
                     /locus_tag="ACHKAY_004011"
     CDS             10175..11221
                     /locus_tag="ACHKAY_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5702596.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            11234..11593
                     /locus_tag="ACHKAY_004012"
     CDS             11234..11593
                     /locus_tag="ACHKAY_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5702597.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            11602..12132
                     /locus_tag="ACHKAY_004013"
     CDS             11602..12132
                     /locus_tag="ACHKAY_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5702598.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWNQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            12374..12571
                     /locus_tag="ACHKAY_004014"
     CDS             12374..12571
                     /locus_tag="ACHKAY_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5702599.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            12706..13419
                     /locus_tag="ACHKAY_004015"
     CDS             12706..13419
                     /locus_tag="ACHKAY_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5702600.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            13448..13523
                     /locus_tag="ACHKAY_004016"
     tRNA            13448..13523
                     /locus_tag="ACHKAY_004016"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13481..13483,aa:Ile,seq:cat)
     gene            13533..13609
                     /locus_tag="ACHKAY_004017"
     tRNA            13533..13609
                     /locus_tag="ACHKAY_004017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13567..13569,aa:Arg,seq:tcg)
     gene            13623..13699
                     /locus_tag="ACHKAY_004018"
     tRNA            13623..13699
                     /locus_tag="ACHKAY_004018"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13657..13659,aa:Arg,seq:tct)
     gene            13869..>14146
                     /locus_tag="ACHKAY_004019"
     CDS             13869..>14146
                     /locus_tag="ACHKAY_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO5702601.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
BASE COUNT         3838 a         3033 c         3805 g         3470 t
ORIGIN      
        1 tcacccgtgg agaactcctt aattaccaac cttagcttcg ttggttagct attaacgcgg
       61 gtatgtaatc attctggcaa tgcttaatgc cgctgctttt tccagattgg tgatatcctg
      121 ctccagagcg gacagatttt cagcctgctt agccctggct tcattggccc atttcaggtc
      181 ctgcgctgcc ttaattttct ggtgcatcca ctcataaagt tcatcatcgg tatagtctgg
      241 cgcgatgatg acgggttctc gtttctgcat gtcggctcct tgtggttagc gctgcctgct
      301 tttaaccacg tcaggcgagg tggtatcctc tgaggggtct gttactcgag aggaaattgg
      361 ttatgaatac aatcaagttt tcttgcccag aatgtggtgg cgaagtcttt gacacatcct
      421 ttaaaccgca tggctctgac agtttcgcgg gagccatctg caaaaattgt ggtcaccttg
      481 taactgaaga tgagtcctcg cagtttgatg acgaaatcgt tgacaatatc ttcggtgcac
      541 tcaccagaga ctttctgaag taaaggcgca taccgcttag ttaccgctct gataattctt
      601 acctgtccgg caatggcgct gatatcaata taaagcgcca tcgctgtttc tttgctgatc
      661 cctggatgcc ttccattctg atgtttgact tcgcccactg agaaatcctc tgtttcccct
      721 taacgccggg gtagcggaac aaaaaacctg ctgcatagtt aaacgtaatc cccgccgtca
      781 tgttcatacg cctcaggctg gctacttaac ccctgaccac tgccgggtaa ctcgaagtat
      841 tgccctgcgt tctgtggggc ggggtgggtt tatggataca atttacaaat taaaatttaa
      901 ctggtcaaca tgtttattat taaatttgta actgcgggct gttgcatcag tcccaaaatg
      961 ggactactat acagttatga agattatctc agttaaaaca ctcagggatt tttgggcgga
     1021 gaatcctgat gcagaacaac cgctaagggc atgggtggat gaggcggcaa aagctgactg
     1081 gaaaagccca gcagacatta aggcacaata ccgaacggcc agtatattga aaaaccggcg
     1141 cgtggtattc aacataaaag gtaatcatta ccgtttaatt gttgcaattg cgtatcagcg
     1201 aggatgggta tttgttaaat tcatcggaaa ccacaaggaa tacgatgcta ttgatgctga
     1261 taccatcgaa ctggagtaag catgaacatc aaacctattc gtacagagca agattatgaa
     1321 gccgcgttgc gtgctgttga acccatgttc gacaatgagc ccgaaattga cacgcctgag
     1381 ggggattttt ttgaggtgat gtgtttgcta atagaggaat atgaaaaaaa acattatccc
     1441 attgagccac catccccaat tgaagctata agatttcgca tggaacagca ggggctgact
     1501 gtgaaagatt tggaacccgc aattgggaaa aaaaatcggg tttatgaggt gttgaatggt
     1561 accagaagcc ttacgttacc aatgattcgc cgtcttcata atcaatttgg tatccccctg
     1621 gaaagcctgg ttggattata aaatctgcta gtcatttgcc tgatgctcgt tccagaaaag
     1681 gaatgcatca ggcagttttg tttttctgcc gcagtaactc ttcaagtttc cgtttataga
     1741 aatcgcgttt ttgctccata tcacgaatga tctgctctgc gtcgctttga ggtaactcat
     1801 ctaaaagcga tatgattttt cgttgttgtt ctgtaagttg cggttggttg tcattactgg
     1861 atacagccat tttatcgccg agagtttctt cttccataaa gaaccaatgg acgggatgtt
     1921 gtgagagctc tgctaatttt tccagtttat ccattcttgg catcacgcct ttcaaccaac
     1981 cttgcacgga ttggggtttt acaccaagac gtcttcccag ctctgactgg tttatattca
     2041 attcctgcaa cacctgctga aggcgtttta caaagatcat caccacccct cgtaaactag
     2101 ttccgcgatc ctacagaaaa aattgataag tggcattaca aatagaagtt gaaatttaaa
     2161 tttaaatttg taattatcgg tgtcatcgta aagttcggag ggaaacatgc aaaaaagtac
     2221 tcaagtgaaa atcctgtcaa taatgagcca atcagaatta gggcgtcgtc ttggtaaaac
     2281 accgcaaacc ataagtgggt ggtttaaaaa acgagtgcca gcggaggagg ttataccagc
     2341 atgtgaggcg cttgactggg gagtaactcc gcatgaattg cgccctgata aataccctaa
     2401 tccaaccgat ggtttacctg ttgagtatca ggctaacgca caagcagcgg cgggagttga
     2461 ttcatgaaaa tcaagcatga gcacatccgc atggcgatga atgcgtggct gctttatccg
     2521 agggtagggc gcaaaaaaat cgctgatgat atagcgacag catattttga gcttgaaatg
     2581 acttatccac caatgcatga cacctctacg acagagggta ttggattgaa catacaaaat
     2641 atttttcgct ggcttgaaaa ggatacgcct gatgctgttg aaaaaattca ggcactaatt
     2701 ccagctatcc tgactgttct tccgcgtgaa ctgcgttatc acctcagtat ttttgacact
     2761 gttgagcgcc gtgcattact ggcggcccag gaagcgttga gcacggcaat tgatgcgcat
     2821 gatgatgcag tccaggcagt ttaccggaaa gcgtatttca gcgacggcgg gtcatccggc
     2881 gagtctgttg tggtgcattg atatttatgc cagacccctg ctgattctgt tgattgggga
     2941 atcacagaat atcaccagag gatggttcgt cacaagatga ggcaattatg gccgcattac
     3001 catacatgca actgtacata gctgattacc tggctgacac catgcatttg tccgcagagg
     3061 agcacggtgc gtatttgttg ctgatgttca attactggca aacagggaaa ccaataccta
     3121 aaaacaggct ggcaaaaatt tcccgtctga ctaacgagcg atgggctgat gtggaaccat
     3181 ccttgcggga gtttttttgc gataacagcg atgaatgggt gcatcttcgg attgaggaag
     3241 atctggcatc agtcagggaa aaattgacca aaaaatcagc cgccggaaaa gcatctgttc
     3301 aggccagaag aagcagaaag gaagcatatg ttcaaacaaa acaagagaga gatttaacag
     3361 gtgttcaaac aaatgttggt gttgtgtttg aacatgacgc aaacacaaaa gcaactaata
     3421 aagatacaga tctaaaagaa ttaaacccca cacataacgt gcgtgaacgc gagagtattc
     3481 cgaccagtga gtcgcatggt gcgccgttgc agacagccga acctgaatac ctggacggcc
     3541 tgagcgaacc gatcgggaaa ttttcgatga ctactgtctg gcagccgtcg ccggattttc
     3601 gacaacgggc agcagtgtgg ggtatggctc tgcctgagcc ggaatttaca cctgctgagc
     3661 ttgccgcatt ccgggattac tggatggcgg aggggaaggt tttcacgcag gttcagtggg
     3721 agcagaaatt tgcccgccac gtgcagcacg tcagggcaca ggtaaaacca gtcagcaagg
     3781 gggtaagcca tgcagcatca ggtggcacgg catcacgggc agttcaggaa atccgggcag
     3841 cacgcgaaca gtgggaacgt gaaaacggat ttatcagcaa cggaaacggc ctggaagctg
     3901 tgggagctta tgggggaggt gtattcgaac cgctggactc agaagaacgg ggccgcacct
     3961 tcgaagctct ggattgccca gattggtgcg atgactgaac agcaaatccg tctggtctgc
     4021 cgtcagtgca tggaccgctg ccgggcgggt gaaacgtggc ccccggacct ggctgagttt
     4081 gttgcgctga tttcggagag tggggcaaat ccatttggtc ttacggtgga tgccgtgatg
     4141 gaagagtacc ggcgctggcg caatgaatcc tggcgatacg acgggagtga taaatacccg
     4201 tggccacagc ctgtgctgta ccacatctgc ctcgaaatgc gtaccagagg gattgagcgc
     4261 cagatgacgc agggtgagtt aaaactactt gcagaacggc aactgacgaa atgggcaaag
     4321 catgttggta acgggatgag tgttccgcca gtgcgacgac aactggaagg ggcgaaacac
     4381 ccgcaagggc caacgccaat tgagcggctg aaacaggaat acgaacgccg gaaggcagct
     4441 ggttttattt gaatctgaga aacgattttg tcggaggaaa ttttaatgga aaccgtattt
     4501 gacgcactga aagcactgaa aagagcctct tcacaggtag tggcggcccg ccttggaatc
     4561 agccgtgaag atgcggtcaa cgaactgtgg aaactgaagc gccgcggtga agcggataac
     4621 aaggggtcga tgtggtggct gattcaggct ggtgaaagtg aaccggtgtc accggtaccg
     4681 aaagtgacag cgcaaatgct gactgaggcg attgaacaac atggcccaca aacggcggat
     4741 gagctggcac tgatgttcgg gattacctcc cgccgggcga attcatcgct ggccatggta
     4801 atcagcaaag ggcgtctgat tcgcgtgaat cagggcggta aatttcgtta ctgcataccg
     4861 ggcgctgatt taccggcaga gccgaaagct gcatccgtag cggaaaccga tggtaaagcc
     4921 tttcctcagc cagcaggtgt tgcgttacca gttcgggaag cagaaacaca ggaagaaata
     4981 aaaacggaaa gtgtggcggt cacagtgcag tcacagccgt cgttcaccag aaaacatccg
     5041 gatggtctga ttttaccatc gctgcatgtg gctaaccgcg agctgcgccg ggcaaaaggt
     5101 caggttcaga agtgggagcg agtctgcgcc gcgctgcggg agctgaacaa gcaccgggat
     5161 attgttcgac agattactga ttcttcccgc cgtgttgtat cggaaaagtg attgccggag
     5221 gcgcttatgg caaaagaatt tacaccagaa gagcgggaag aagtgaaggc gcgcattgtg
     5281 gaattcgtgc gcctgagcgg acgagaaact tttcgacaac tggcagataa aacgggtgtc
     5341 agtaagaccg ctattcgtcg tttatctggt gcgcttgcgg ccagtggtga tgtctggctc
     5401 tctggttgcg gggtatttcc atcagagcag gcgtatcgcg tatggcgtaa gacaccggag
     5461 aaggctgctg acccgacact gattcgaaag ttacctgacg gagaaatacg tcgttacaac
     5521 agacggcaga acataatttg tcgtgagtgc cgccagagcg aagttatgca gcgtgtgctg
     5581 gcgttctatc ggggaaactt tcaggaggtg atggagtgag ggtgagagtt tatattgccg
     5641 gtccaatgac gggatatgaa aatttcaacc gtgaggcgtt tcacaaggcg gaagaggaac
     5701 tgaaacggga agggcatacc gtcttaactc cggcagtact tccggacggg ctgacacagc
     5761 cgcactacat ggatatttgc atggcaatga ttcgttgtgt ggatgcgatt tacatgctga
     5821 atggctggca gcggtcagcg ggcgctaagg cagagctggc actggcggag aaactggggc
     5881 atgcggtgat ttatcaggag gtggctcgat gagagaggtt aactatgagg cgcttcgtga
     5941 ggcagcacaa aactatcagt cgacgctggc gtggtatcag gctaccccgg acagcccaaa
     6001 tgctgaacgg gattgtgatg cggctcttgc tgcgtttaag cgtcatatcc gtcatcggga
     6061 agcggatatt atcgctggtt tgctggatgg actggaagaa gcaaaatcac aactcaacga
     6121 gcagcgtgag tattacgaag gcgttatctc tgatgggagc aagcgtattg ctgaactgga
     6181 tgcgcgggaa gttcaattac cgactcgcta cgaccttcga tatggacacc cgataaatgc
     6241 agatgagcga catgtcatga tacctaaaga aaatggcagt tggctttacc tgattgacct
     6301 agaacacgca ttacgcgtcg ctggcattcg catcaaagga gaggagcatg gaaataaaac
     6361 cagaggatga gttaagcaat atcgttttat ttccggtaaa agaggatgac cctcgtaatc
     6421 aggttaattt tctttatgag ccatcggaaa gaccatattg tcatcacgcc tctgttcggg
     6481 ttgacgaaaa agagcgccag gtccgctgta aaatctgcgg tgcggttgtg gagccgtttg
     6541 actggatgct ctctgtggcg aaaagagaaa ccagactggc agatgatgta aggctattgc
     6601 gccaggagga acaggaaagg cggaaaaata tagaaaagtt aattcagatt gaacgtaacg
     6661 cgaaagcgcg gatacgcagg gcgacaaaat ccagaactga ataaataaat ttagcgctgt
     6721 aaataaaatc taatcctgaa ctggaggtat atttatgtta aatacacaga aagccattaa
     6781 tgcggaaaaa tataacgagt gggcaagaaa attctctgag cagattttta aaattactgg
     6841 cgatgagaat gcggcaaaaa atgaattaga accgtggaca cctgaaggaa acgcaccaaa
     6901 ttattgctgg tgggaggttg atccggttga tgttgcaaat gaagccatga gttaccacaa
     6961 cgattaatgt caggaggccg cccgaaaggg cggtagttaa atgcgaaagt ttaaaataat
     7021 tattgaaacg ggaatagccg gtggagattt cgaggatgaa ttcgaagtgg atgatgatgc
     7081 gacgcctgat gaaatacatg acgaagcaaa agatattttc tttaactact gcaattattc
     7141 atatcacgaa ataaaagacg aagaggaaga acaaaatggc tgattttggt tcaactaaat
     7201 acagcgtcag ttttgaagaa tggcatgaac tgttaatgga atatgcagag ttacgtggtg
     7261 gcagtgctgc tgatgctgaa gcatggcgtg atgattatga agcaggaaaa aatccggtcg
     7321 aagcatattg tgatgagtgg ggcgatgaat gagcgagatt gactatcagg cgctgcgtga
     7381 agcagcagag aaagcaacta aaggatgcta catcgtaggg catacatcgg gcaatcagca
     7441 tgggaatata acaggagttt ttgtttgtca aaaatggaaa ggagagcccg gtggcgtaat
     7501 tgcagaatgt catgttaact gcctggttga aacagatgtt caggcttatg caaacgctga
     7561 atttattgct gcttttaatc caaatgttgc gctggcacta ctggatgaac gggaagccca
     7621 aaacaaacga attgcagagc tgaaagctaa tcttgtggcg ctggcggcgg agaatgcggt
     7681 aataaagtct gcaattccag aaccacggga tattgaggac gacaatgaca atatggatga
     7741 cgtatctctt gctgaagatt tcgggttcaa tcatgcaata gaacggatga ggagacggat
     7801 tcctgaaact ccggccaccg acgctttcct ggctgaagta cgggcggggt tgtttaacga
     7861 gctttgcgcg gcgtttgtca ggtacgaaaa agttgcagga ctggatgata gcgatacagt
     7921 aacgcttaag gaagcgacag aagcattgct ggattgtgcg gaacagctta tcgcgcctga
     7981 ataattaaat ttagttaaaa atttaatcct taaccggagg gatttctgca ccctcaaaac
     8041 atcaggaggc cgcctgaaag ggcggtaaga aatgactaca ttattcagaa aagaatatcc
     8101 gagaaaaagt agagcgacag aatttttgtt tctcattata tttatcgtgt tgatgatacc
     8161 gatacccccg ctattactgg tctggtttat cgtgaaaata attgagccag ttattgaatt
     8221 gtataacgac gtggtgtggg cgtcgttcag cacactgcac aataaaatta atccgtataa
     8281 ggaaagctga tatggcactg acgaaaaaac aacgtgcaga gctgcgcatg aagttcggcg
     8341 gtcgctgtgc ttattgcggc tgcgaacttg gcgaaaagtg gcatgcagac catgtaaaac
     8401 cggtcattcg ttttgatgga aatatgcttc accaggaacg tgacgatata tccaacatgg
     8461 ttccggcatg ccacccatgc aatctgcaca agcattgcag tagtctggaa gattatcggc
     8521 gaattatcag tgatggtcgt cgtgaattcc ttgcgtccgg gaaaggcaaa gcgctggttc
     8581 gtatgggatt ggttgaaatg aaatctgacc cagttgtgtt ctggtttgaa aaatatcaag
     8641 aaggggctac ggcatgacga cttttaccag agagcagtta atagctcacg cagaggagac
     8701 tattgaaaca cagagactgt gcataccggg cacaatcgac catgacatca tccgcacata
     8761 taagatggat attgctgttc tggaaatcgc actggtatcg ctggcagcag agccagccgg
     8821 taaattgcat gaatacaaac cagtgggata tcagcgtctg gtcgatgagt taaccatgct
     8881 ggtaaagcag ttaacctggc aactgaggaa agcgaagcca gactgcaaat taccggataa
     8941 ggcgatgagt tatctggagc ggaacggact gataagcgtg gaggatattt tacgatgacc
     9001 tggcctgaag cattcacaac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     9061 atttgccgct ggggataaat cgccgaaaaa agatcccgac ataaacatga gccgggatct
     9121 ttgatttata tagcctacga atccgccagt aagagagggg gcggacggtt aattctaaca
     9181 ccggaatgat gtgggtaaaa gtttataaga aatcggtttc ataactttgc ccaccatgat
     9241 agataccgac aataaagact tttctgctat caacggcaaa agcaataatc gttctgtggc
     9301 ggaaatgagt tacccgcatc ccctggcgaa tatcatcgcg tttattgccc cgatgcggga
     9361 atgtagaaaa cccatcaaga taatcaagaa gcgcattggc ataattgtca gcaatgacgt
     9421 tccctgcttt ctccgttata tacctgtgca ggttgattat ttgttgttcg gcctcaggag
     9481 taatgatgac ttcatatgtc atgcagatta cttcccggat cgaatcgcgg cgcgaacctg
     9541 tgaaatggag cgtccgttgt ttggattttc gcggatagaa tcaagagagg gggcggctga
     9601 atgcgttaac cacgcttcga ttgctttatc gcgctcattc agtgcgcgaa gcccttcacg
     9661 aatgacctcg ctttctgaag cataggcacc ggaagccaca cgggcgcgca ccatgtcagc
     9721 catttcgtta gttaatgtaa tgctgaattg ttgggttgta cgcatggtaa acctcacgga
     9781 gtaggataga acaccattcg atgatagcac gttgcctgtt gacgacaaca gaaatcagag
     9841 acaatattgc cgcacgccag cttgaacaac tggcacctgc tgcgccagca gagaaaaccg
     9901 atggcgcaca ataccaaaca tcacaattct gatatcgccc ctgccagcag gcaagggcgg
     9961 tgttctcaca cattcaaata tgactggtat cagcacgatc cctgcactga agaacaggcc
    10021 gaatggctga ttcataacta ccgcagacgt gggtatgagt ttaagaaagc ccttagcctc
    10081 gactaccgtc actggataat ctacgtcagg ctcctttatt ccgaacgccc gccgcgtccg
    10141 tcccgcacat tccagcaacg catctggagg taacgtgcgg gtattacttc gacctgttct
    10201 ggtaccggaa ctcgggctgg tgatcgttaa gccgggtcgt gaatccatgc cggtattcca
    10261 caatacccgg gtactggtgg agccggaacc gaaaagcatg cgtaatctgc cgtccggggt
    10321 tgttcctgcc gttcgccagc cgctagtgga agacaaaaca ttgctgccgt ttttcagtaa
    10381 cgcacgggtg attcgtgctg ctggtggtgc tggtgcattg tctgactggc tgttgcgcca
    10441 tattaaatcc tgccagtggc cacacggcga ttatcatcac agcgaaaccg tcattcaccg
    10501 ttatggtacc ggcgcaatgg tgttgtgctg gcactgcgac aaccagctgc gcgaccagac
    10561 atccgaatca ctcgggcagc ttgctcaaca aaatctgaca gcctggatga ttgacgtcat
    10621 acgtcacgca ataagtggtg cacaggaacg ggaattatct ctggctgaat tatcctggtg
    10681 ggcggtccgc aatcaggtgg cggacgcgct accggaagcg gtattacgtc gttcgctggg
    10741 gttgcgtgcg gaaaaaatcc gctcaatgta ccgtgaaagc gacatcgtac cgggagagca
    10801 gaccgccacc agcatactga agcagcgcac aaaaaatctt gcgccgctgc ctcacgctca
    10861 ccagcaaaaa ccgccacagg aaaagacggt ggtcagcatt gccgttgatc ctgagtctcc
    10921 ggaatctttc atgaaacgac ctaaacgtcg ccgctgggtt aacgagaaat acacgcactg
    10981 ggtgaagaca cagccgtgtg cgtgttgtgg taagcctgct gacgatccgc atcacctgat
    11041 tggtcatggt caggggggaa tggggacaaa atctcacgat attttcacgc taccgctgtg
    11101 tcgggagcat cacaacgagc ttcatgcgga tccgctggcg ttcgaagaaa agcatggttc
    11161 tcaggttgat ttaatttttc gttttcttga tcacgccttt gcaactggcg tgcttgggta
    11221 aaagaggtga ctgatgctca tagatttggt tttaccttac ccgccgacgg tgaacactta
    11281 ctggcgacgc cgtggcagca catattttat ctcggaggag ggaaagcgtt atcgccgggc
    11341 tgtggcgctt attgttcgcc agcagcggct gaaattaagc ctgtccggaa ggctggcgat
    11401 aaaggtgatt gcagagccac cggataagcg ccgccgtgac ctggacaaca ttctgaaagc
    11461 accgctggat gcgctgacgc atgcggaagt gctcattgat gacgagcagt ttgatgaaat
    11521 caatattgtg cgcggtcagc cagtatctgg tggacggctg ggtgtgaaga tttacaaaat
    11581 tgagagtgag tgagcgtaaa tatgatatat ccggaaatta caggcaaaag cggcgaacat
    11641 ttacgcctga acacgctgga agcagtctgg aaccagggga aattacggat gtgggggcgg
    11701 tggtcgtata tcggtggggg taaatccgga aatatgttta accggttact ggtttcgaaa
    11761 aagctgacga aaacagcagt taatgaggtt ttacgcagca tgaagaaatc cgggctggaa
    11821 aaaccggaac ttgaggcatt ttttcgggat atgaccagag ggaagcagaa gagctggttg
    11881 tcacattgta cagacacaga ggcgttgatt attgatcgcg ttatcagtga ggtgcttggg
    11941 gaatatcccg ggctaatcaa tattctccgg caaaggtacg aaggacgggg aatgagtaag
    12001 agaaaaatgg cagaatgttt aaatcgtact cacccggaat ggtgtttcag cacatgtgag
    12061 aaacgtattg caggttggtt agccgtggct gaacacatgc tttatgtacc tatgcacgat
    12121 tcatttcgat aaaaaaagct tgctttttta cgcagaaaca gcttgaattc ctgtaagctt
    12181 cgcaaagctg tatcgcgagg cgaaatgcaa gttttttcgc acaaggaaac caccggaagg
    12241 tggttttttt gtgtccgtaa tatacagcag cgcaataaat tcgctggtgg ttattaatac
    12301 cgttctttca gcttgctggc tttttcgaca agagttattg gtgtgtcacg ttaaccgaaa
    12361 aagggaaaaa gacatgctga aacagcagga tatgaccgaa accgccagag tggtgtttaa
    12421 tgaattaagc gttaccgaac cggcgacagt cggggaggtt gcgcagaata cttacctttc
    12481 acgcgaacgc tgccagttaa tactgaccca gcttgttatg gcgggtctgg cagactatca
    12541 gtgcggttgt tacagacgcc ttcagtcctg aaggcttttt atttgtggtg aatgggcggc
    12601 tggtgggggg gcgacacctg tcagtccttt gcttatgtgt tgatgataat ttaccttttg
    12661 gggctataat tgagctaacc aattgctaat gaaagtaaaa ttataatggc tgttgtctgt
    12721 tcagttatca tggtttgctc cccaattaat atttttcttg aaaaggatac gttgtcactt
    12781 aagccaggct cagtcgttct ggccaccaaa tgcatcaggg agcttttcct tatgcattat
    12841 ggcaaagtta aaattgtcga tataagcgaa tccgtcgtaa gtcaatatct ggaaagtcag
    12901 cataagctga cgaggactcg tctgactgac attccgcttt acctgttgct ggaacccaac
    12961 aatcctgcgt tggctgcggc tttaattacc agccagggat tttccggaga ggccacggat
    13021 atgtttctta tgatggcctg cctgtctctg tttgaaacag atgaacggat gtcattgttt
    13081 ttaagtggat gtttatccag cataagtgcc aaagtcaggg cgataattca gacagatata
    13141 tcagcaagct ggacgcttgg tgcgattgct ctacagttgc atatgagtga gagtttgtta
    13201 aagacaaaac tgaaaaatga agggggcatg ttcagtcgct tgttgctgga agagcggatg
    13261 cgtgttgctg taaatatgtt atgttcccgg catggatatg gacaggctat agcagaaaaa
    13321 tgcggttatt caagcaggtc ctactttatt tctgtatttc accgctatta tggcttcccg
    13381 ccagacagat atgtatccag gcaagggctt gattattgat tttcatctga ttattatttt
    13441 ttggctcggc cctttagctc agtggtgaga gcgagcgact cataatcgcc aggtcgctgg
    13501 ttcaaatcca gcaagggcca ccatatcaca taccgccatt agctcatcgg gacagagcgc
    13561 cagccttcga agctggctgc gcggggttca agtccccgat ggcggtccat tatcagcatc
    13621 atgcgttgtt agctcagtcg gacagagcaa ttgccttcta agcaatcggt cagtggttcg
    13681 actccactac aacgcgccac acttattttc caggctcgct tcggcgggcc ttttttgtat
    13741 ctgcgccacg cccggcgcat atcaaccaca gagcctttcg ggggtgagct tacggagtgg
    13801 tcagtgtgac tttctctgtg ggcagatcgc tcccgggcgt tggctcaccc acccaaagga
    13861 acgtcacgat gtttggaatc ttcaaaaaga aaacccgcag agcggcagcg gaaattaaaa
    13921 agtttgagaa acgcgatctg gcacaggcgg tgattaacgc tgcatacctg gtggcctatg
    13981 cagatggtga atgcgaggca tccgagaaag cgaagatcga acaggtctta cgtaatcagc
    14041 ctgcgttgtc tgcgtttacc tcggaaatta atgcgattag cgcaactatt atcggtcagc
    14101 tggataccaa ttttaaaatt ggtcgtcgtg ccgcgttacg cgagat
//
LOCUS       DAZFKM010000131        14068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFKM010000131 DAZFKM010000000
VERSION     DAZFKM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHKAY_004020"
     CDS             complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHKAY_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO5702602.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHKAY_004021"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHKAY_004021"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO5702603.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHKAY_004022"
     CDS             complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHKAY_004022"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO5702604.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHKAY_004023"
     CDS             complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHKAY_004023"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO5702605.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5271..5390)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5326..5391)
                     /gene="thrL"
                     /locus_tag="ACHKAY_004024"
     CDS             complement(5326..5391)
                     /gene="thrL"
                     /locus_tag="ACHKAY_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO5702606.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(5605..6291)
                     /gene="yjtD"
                     /locus_tag="ACHKAY_004025"
     CDS             complement(5605..6291)
                     /gene="yjtD"
                     /locus_tag="ACHKAY_004025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO5702607.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6691..6831)
                     /gene="yjjY"
                     /locus_tag="ACHKAY_004026"
     CDS             complement(6691..6831)
                     /gene="yjjY"
                     /locus_tag="ACHKAY_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO5702608.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6927..7643
                     /gene="arcA"
                     /locus_tag="ACHKAY_004027"
     CDS             6927..7643
                     /gene="arcA"
                     /locus_tag="ACHKAY_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO5702609.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7703..9055)
                     /gene="creD"
                     /locus_tag="ACHKAY_004028"
     CDS             complement(7703..9055)
                     /gene="creD"
                     /locus_tag="ACHKAY_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO5702610.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9113..10537)
                     /gene="creC"
                     /locus_tag="ACHKAY_004029"
     CDS             complement(9113..10537)
                     /gene="creC"
                     /locus_tag="ACHKAY_004029"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO5702611.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10537..11226)
                     /gene="creB"
                     /locus_tag="ACHKAY_004030"
     CDS             complement(10537..11226)
                     /gene="creB"
                     /locus_tag="ACHKAY_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO5702612.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11239..11712)
                     /gene="creA"
                     /locus_tag="ACHKAY_004031"
     CDS             complement(11239..11712)
                     /gene="creA"
                     /locus_tag="ACHKAY_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO5702613.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11923..12792
                     /gene="robA"
                     /locus_tag="ACHKAY_004032"
     CDS             11923..12792
                     /gene="robA"
                     /locus_tag="ACHKAY_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO5702614.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12789..13436)
                     /gene="gpmB"
                     /locus_tag="ACHKAY_004033"
     CDS             complement(12789..13436)
                     /gene="gpmB"
                     /locus_tag="ACHKAY_004033"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO5702615.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13488..14009
                     /gene="yjjX"
                     /locus_tag="ACHKAY_004034"
     CDS             13488..14009
                     /gene="yjjX"
                     /locus_tag="ACHKAY_004034"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO5702616.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         3439 a         3845 c         3407 g         3377 t
ORIGIN      
        1 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgatgag ctttcttatg gtgacgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg accgccatcc cagtaataac cgcgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgcgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacgg cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttctctc catattttct tcacaaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatg ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg ggaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggtaccgaaa caaccagctg gttgcgcgcc
     1141 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca atgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcac ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acacagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc tggcagtaat cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagcctgcc
     2161 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagcccgg
     2221 gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac
     2281 ccaggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accacctaga
     2341 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc cagctcgccc
     2401 atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt catcgccatc
     2461 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg catattcttt
     2521 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
     2581 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg cccgaggttg
     2641 ttgagactga atatctctgc cgcctcaacc gtaactacat ctccgagcaa tgcaccatca
     2701 acgggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
     2761 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gcagcagatc
     2821 ggcaaagaca ccagcagcgg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
     2881 cagcggctga taatagtggc tatagaaggc cagggcgttt tcgccatttt tcactttgaa
     2941 cagcggatca ttaccatcca cttcggcaat cttcacgcga cagacgccat cttcatcaat
     3001 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
     3061 gcgatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa
     3121 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
     3181 acgggcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat ctcgcggatc
     3241 cggttcggta taacccattt cccgtgccag cgtggtcgcc tcggagaaac tcatgccttc
     3301 gtctaacttg ccgaagatat aagaaagcga tcctgaaaga atgccggaga acttcatcaa
     3361 ttcatcacca gcattgagca gattttgcag gttttcaata accggtaatc cagccccaac
     3421 gttggtgtca tagaggaatt tccgccgcga tttttccgcc gcatgacgca acagatggta
     3481 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
     3541 caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
     3601 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
     3661 ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat tggtgagcag
     3721 tgccttcgag ttggcaacac cgcagacacg taagtcgata tgtttattct tcagccagct
     3781 ttgttgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
     3841 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
     3901 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatagc
     3961 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
     4021 ggtacgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
     4081 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagcccg
     4141 cacacagtcg ctttgcggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
     4201 cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac
     4261 cattcctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
     4321 gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac ctggtgcttg
     4381 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
     4441 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
     4501 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
     4561 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
     4621 gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc
     4681 tgccatcagc accatgtgat cagccggaat acgacttgcc gcaatacggc gggtggactc
     4741 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agtagttttt cgaccggatc
     4801 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
     4861 tttctcgcca cggcaaatca gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact
     4921 aatgccatgc aggacatgtt ttatttgggc aaattcctgg tcgacgaaag ttttcaattg
     4981 cgccagcggg aatcccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
     5041 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
     5101 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
     5161 ggcattgctt tccagaatat cggcaacccg cagaaaacgt tctgcatttg ccactgatgt
     5221 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag
     5281 cccgcactgt caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt
     5341 acctgtggta atggtgatgg tggtgataat ggtggtgcta atgcgtttca tggatgttgt
     5401 gtactctgta atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga
     5461 cctaagtcaa taaaatttta atttactcac ggcaggtaac cagttcagaa gctgctgtca
     5521 gagactcttt ttttaatcca cacagagaca tattgcccat tgcagtcaga atgaaaagct
     5581 gaaaaatact tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa
     5641 acggtgcaac attgccgtgt ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc
     5701 gaccagtttt atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg
     5761 taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt ttcgtcggtt gttgtattaa
     5821 tgttgctaat tgatagcaat agaccatcac tgcctgcccc agattgagcg aaggataatc
     5881 cgccaccatc ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcaaccc
     5941 ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa
     6001 cagcggcacc agttcaactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt
     6061 ggtggcgaca gtgaaatcga catcgtgtaa cgattcaacc agtgtcggga aaactttaat
     6121 attatcaata atatcaccag atccatgtgc gacccagcga gtggctggct ccagatgtgc
     6181 ctgactatcg acaatccgca gttcgctaaa ccccatcgtt ttcattgccc gcgccgctgc
     6241 cccaatattt tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact
     6301 cttcttgacc aaatgaccgc gaacagggtg atcactgtca acttattacg cggtgcgaat
     6361 ttacaaatcc ttaacgtaag tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa
     6421 ctatttcctg actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta
     6481 cttaggataa ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg
     6541 tatgatgcaa ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat
     6601 gatttcatca aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt
     6661 tttattgggt gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac
     6721 agccgttttt gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt
     6781 gttgacgttg atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg
     6841 atcatgttaa tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga
     6901 tttagttggc aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg
     6961 agttggtaac acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg
     7021 aagcgacaga tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga
     7081 tcatggatat caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc
     7141 aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg
     7201 gcctcgaaat cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga
     7261 ttcgtgcacg caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc
     7321 gtagcgttga aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga
     7381 tcggccctga tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact
     7441 tctgtgaaaa cccaggcaaa attcagtccc gtgctgaact gctgaagaaa atgaccggcc
     7501 gtgagctgaa accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt
     7561 tcgaatctac gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct
     7621 tctgcggtga tctggaagat taatcggcgt taccaccgtc aaaaaaaatg gcgcttttta
     7681 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt
     7741 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa
     7801 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
     7861 cagagttgag cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca
     7921 tgctgttgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
     7981 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
     8041 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
     8101 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
     8161 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
     8221 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
     8281 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt
     8341 tggctggcaa aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
     8401 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagggcca
     8461 tattcagctt caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
     8521 gcccgccttg ctctaacccg gtgccgggtt caatggtcag cgccgttccg ttaacttcag
     8581 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
     8641 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
     8701 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
     8761 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
     8821 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
     8881 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
     8941 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
     9001 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
     9061 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
     9121 agtgacggtg aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
     9181 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
     9241 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
     9301 caggaatacc actaccggta tccagcacct taagggcgac gtgttcctga tccacttcgg
     9361 cgcttagcgt tatgcgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc
     9421 ccagcgcctg ctccagtaac gccggttcag cagcaacatt aacctcagtg ggcataacat
     9481 gtagagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
     9541 atgccgccac atcaacaaca gtcagaacga cttcctgacg attctccagt cttgcctggc
     9601 gtagtaacgt ttctaccaat gcctgcatac gcgcattttg cgtcagaatg ttgtcggtaa
     9661 aacgagccac cacttccggt ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
     9721 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
     9781 tcccttccag cttcacgcgc atactttcca gcgcctgcgc aagcttacgc aactcgctac
     9841 tgccgagttc aggaagagga acgggcttat tgtcagtgac ggaatcggca tagcgagtga
     9901 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
     9961 ccagcaaaat ggcgctggcc cataaaattc gccgctcgct acgcttaatg accggagcca
    10021 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg
    10081 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    10141 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa
    10201 cggctttatt tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat
    10261 attcattgcg cactttgtta atgccaccga tattggcgcg aaacgggcga tgttgtagct
    10321 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    10381 ccagctccgc cagcaacgtt gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc
    10441 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    10501 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc
    10561 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    10621 caacgtttta atgtgggtat caatggtgcg atcgtaggtg tcctgcgcat cttcccacac
    10681 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    10741 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    10801 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt
    10861 cacccgacgc agtaaggtgc gcacccttgc gcacacttcg cggggtgaaa acggtttagc
    10921 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    10981 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc ataattcaaa
    11041 gccgctgata tcaggcaaac caacatcgag aatcatgacg tcgggagcct gctggcgagc
    11101 tttatccagc accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    11161 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    11221 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    11281 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    11341 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    11401 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    11461 ttgctgacaa gaaatagccg catcggaggt atcttccgcc agacccaatc cccctttaat
    11521 acccccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    11581 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    11641 aatctcctct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    11701 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    11761 aataacatat tgattaagaa caaaatctca atacttttat ttccgtcaag ccctaaaaca
    11821 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    11881 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    11941 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    12001 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    12061 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    12121 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    12181 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    12241 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cactatgcca
    12301 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    12361 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    12421 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    12481 agtcaggata aagacgacga acaagaagta ttctatacca ccgcgttagc ccaggatcag
    12541 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    12601 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atatggaaca
    12661 tgcatgccaa tgcttaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    12721 gcagaagatg ccaaagcagg agatcgccca attaatctgc gctgtgaact gctgattccg
    12781 atccgtcgtt aacgctgcaa ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    12841 gcggtttcaa caacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    12901 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    12961 ctcaccaggc atcccagtgc aataccatga ctcaccagca gcggtcggct tccctgcggt
    13021 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    13081 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    13141 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    13201 cgagaatcaa agatgatgtc gcagccgcag gcctgggcga tgatttccgc cgtacgccgg
    13261 gtgcgtccta aatcgctact gatgatatgc gtaatgccaa gctctttggc acgggttgcc
    13321 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    13381 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    13441 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    13501 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    13561 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    13621 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    13681 tacttccaga ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt
    13741 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gctactttgc
    13801 cgttgcctgc ggtgattctg gaaaaagtgc gtgaaggcga ggcgctcggc cccgtgatgt
    13861 cgcgttatac cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg
    13921 ccgggaaact cacccgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt
    13981 ttcataatgc cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca
    14041 ttcggtgcac gatgcctgat gcgacgct
//
LOCUS       DAZFKM010000132        13906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFKM010000132 DAZFKM010000000
VERSION     DAZFKM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1892)
                     /locus_tag="ACHKAY_004035"
     CDS             complement(<1..1892)
                     /locus_tag="ACHKAY_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5702617.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGH"
     gene            complement(1986..2177)
                     /gene="ydfD"
                     /locus_tag="ACHKAY_004036"
     CDS             complement(1986..2177)
                     /gene="ydfD"
                     /locus_tag="ACHKAY_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO5702618.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(2174..2362)
                     /gene="dicB"
                     /locus_tag="ACHKAY_004037"
                     /gene_synonym="ftsT"
     CDS             complement(2174..2362)
                     /gene="dicB"
                     /locus_tag="ACHKAY_004037"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5702619.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(3323..3424)
                     /gene="ydfB"
                     /locus_tag="ACHKAY_004038"
                     /pseudo
     CDS             complement(3323..3424)
                     /gene="ydfB"
                     /locus_tag="ACHKAY_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(3426..3581)
                     /gene="ydfA"
                     /locus_tag="ACHKAY_004039"
     CDS             complement(3426..3581)
                     /gene="ydfA"
                     /locus_tag="ACHKAY_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5702620.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(3774..4181)
                     /locus_tag="ACHKAY_004040"
     CDS             complement(3774..4181)
                     /locus_tag="ACHKAY_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5702621.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4259..4486
                     /locus_tag="ACHKAY_004041"
     CDS             4259..4486
                     /locus_tag="ACHKAY_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5702622.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            4470..4991
                     /locus_tag="ACHKAY_004042"
     CDS             4470..4991
                     /locus_tag="ACHKAY_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5702623.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            4972..5937
                     /locus_tag="ACHKAY_004043"
     CDS             4972..5937
                     /locus_tag="ACHKAY_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702624.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            5978..6397
                     /locus_tag="ACHKAY_004044"
     CDS             5978..6397
                     /locus_tag="ACHKAY_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5702625.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            6452..7549
                     /locus_tag="ACHKAY_004045"
                     /pseudo
     CDS             6452..7549
                     /locus_tag="ACHKAY_004045"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            7622..7771
                     /gene="ydfW"
                     /locus_tag="ACHKAY_004046"
     CDS             7622..7771
                     /gene="ydfW"
                     /locus_tag="ACHKAY_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO5702626.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(8208..8540)
                     /gene="flxA"
                     /locus_tag="ACHKAY_004047"
     CDS             complement(8208..8540)
                     /gene="flxA"
                     /locus_tag="ACHKAY_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO5702627.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(8743..9048)
                     /gene="ydfV"
                     /locus_tag="ACHKAY_004048"
     CDS             complement(8743..9048)
                     /gene="ydfV"
                     /locus_tag="ACHKAY_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO5702628.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            9073..9312
                     /gene="relB"
                     /locus_tag="ACHKAY_004049"
     CDS             9073..9312
                     /gene="relB"
                     /locus_tag="ACHKAY_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO5702629.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            9312..9599
                     /gene="relE"
                     /locus_tag="ACHKAY_004050"
     CDS             9312..9599
                     /gene="relE"
                     /locus_tag="ACHKAY_004050"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO5702630.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            9671..9826
                     /gene="hokD"
                     /locus_tag="ACHKAY_004051"
     CDS             9671..9826
                     /gene="hokD"
                     /locus_tag="ACHKAY_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5702631.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            10043..10294
                     /gene="rem"
                     /locus_tag="ACHKAY_004052"
     CDS             10043..10294
                     /gene="rem"
                     /locus_tag="ACHKAY_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO5702632.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            10361..10639
                     /locus_tag="ACHKAY_004053"
     CDS             10361..10639
                     /locus_tag="ACHKAY_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702633.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            10641..11690
                     /gene="ydfU"
                     /locus_tag="ACHKAY_004054"
     CDS             10641..11690
                     /gene="ydfU"
                     /locus_tag="ACHKAY_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5702634.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            11704..12457
                     /gene="ydfT"
                     /locus_tag="ACHKAY_004055"
                     /pseudo
     CDS             11704..12457
                     /gene="ydfT"
                     /locus_tag="ACHKAY_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(12735..12824)
                     /locus_tag="ACHKAY_004056"
     CDS             complement(12735..12824)
                     /locus_tag="ACHKAY_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702635.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(12879..13091)
                     /gene="cspF"
                     /locus_tag="ACHKAY_004057"
     CDS             complement(12879..13091)
                     /gene="cspF"
                     /locus_tag="ACHKAY_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO5702636.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            13392..13607
                     /gene="cspB"
                     /locus_tag="ACHKAY_004058"
     CDS             13392..13607
                     /gene="cspB"
                     /locus_tag="ACHKAY_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO5702637.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
BASE COUNT         3630 a         3036 c         3434 g         3806 t
ORIGIN      
        1 tgatgaccgt tcaccttaat tgggtgatct gccgtctcgc cagtttcatt atcgctattg
       61 tgatgctgat tgccgccagt gttctccttg tgtggatgtt cagcgccttc catttcctcc
      121 ggatcatctt cctgaacttc aacctgatac tcttcatcga atgtttcttg gtatgttgcg
      181 tcgcccatca ccgcgccaca atcagggcag ttgccgccgc cggtctgacc gcaggcggtg
      241 caggcttttt ccacttcctg ttgcgccact ggttcaggct gtttcgtttc tggctcgttt
      301 tgtaacgcat ttggactgtt ttgttccgct ttttggtagt tccgttccga ttcatgctgg
      361 ttctggttca cagaatcgcg ggtctggatc cccttaaccc atttcggatc attcgggtca
      421 ctaatccctt caacaaattc accacgtgat gcagcaagca acttatcggc gtcaggctgg
      481 ctgatattgg ctgcctgcat aattttgttt acttcgtcag cggtaacttt tatcggctct
      541 ggttgttctg aatcttcagc ggtatctaca ttttgcggta agcccgtgta tgtgccattt
      601 tttcgggcaa aatattcttc ttttgtgatt tcagtggcgc cagcagccag tgccttatcc
      661 agaccagaaa gtttgtttgc gcgaccgtat ttttctccgt ccttatctgc gaagaggaaa
      721 tagaacggcc cctcacgctc tacagatggt tcagcttccg gcgcggtttc attttttggg
      781 atatcagata cctcagtttc cactgcatca gtttgtgttt ctgatgactg gagaacatca
      841 acagtgccca ggtctgtttc ttcattctca aacacgccct ttgtcgtcag gtattcgcag
      901 atatatttgt tcagtgctac gggatctttg tgaatgtcga tcggacgctc acggacaagg
      961 ccaaaaatag tttggcggtc gtagcgaagg gcatcaggct gtttgcgcat tgatgccgag
     1021 atacgcttcc agtcttcgcg gtcgttgtcg ataacttctt tttttgccca gcgatggatg
     1081 ctgccgtcaa tgtttccggc atccacatca ccaggccaga gagcgtaggc cagttcgtca
     1141 tccagtgttt tccatgtctg cttgtattcg cgatgaatgg cagcaatgac cgggctgatt
     1201 tttcctgttg aattttcagt gtactgttga ttggctctgg cgcgggcgag atcaacaaca
     1261 gacgtgtatt ttccggtttc cttgcgttca ccttcgcgac gttttttcca gatgcgcatc
     1321 tctgcctgaa tttcgggcca tttagcacca ggaatacatt tatgcttaac ccacccaatg
     1381 gcgtgcaact taagctccgg atacatggcg ttaacttctg gcattttcat caacgcttca
     1441 acgatatgtc cgtcgaatgt tgccatgtct tcctgcaaca attcctgtgc gctaataacc
     1501 atatcaacgg tgatgttttc acatgtgtcg aacttaacca tgacagcgtt ctgtacttca
     1561 ggggccagct tgtcaaaagt gacgttcatc ggatcggatt cagtctcaac cgggacaaaa
     1621 gaagcagact cctcatccca gcggttttcc tgcatatatt cagcatccca tgaatcgagg
     1681 gcagggcggg gtatgccagg tttatcctcg cagacaataa atttataagc gcagtcctga
     1741 gcagccggat aatgttccag gaactgccag tgaaattttg ctcgagcacg gcgttcgtcg
     1801 ccagcttcaa tggctgtggc tacagccaca gagccttctt cccttgttgc cagttcgtca
     1861 ggaatagcgg cgcaaataaa gactttactc attttgtttt aacctcatga cagatttaag
     1921 gatgaacaaa tccctgccat tgctggcata taagaatgaa accggatatt tattacggaa
     1981 ctgttttaaa gacctgccgg gatttcgata ttatcctggt gaataacttt atcgaccggg
     2041 taacagttac cgggaatttt ctgttcggtt gctgcagtca tacactcctg cattgtcctg
     2101 tgaacactga ctgcaatatc aactggcact ccggaaacaa gaaaaactgt cagaacaagc
     2161 gcaaatgctg aattcattgt gcacatcctt ttggcatcag acgtaaacga gccagcattg
     2221 aaacaatgca tattttattt aatagctccc gttcttgttt tctcttgtta atggcatctt
     2281 cagtaaatac agggttactg atagtgacac caatttcaaa acaaccttca gacgtattaa
     2341 cgtttggtaa taacgttttc attatcgcgt cctcaacaat gaattttgtg atgcagtgcc
     2401 tggtgcctcc aggtgacgtt aaccagttaa caattaacgt cggatacaga gaatccaccc
     2461 ataacactgt ttttggtttt aactgttccg cgtgcgctta gccgtattca ccgcatcaca
     2521 aaattcactt taaaaagggc ggcagagcag tcacggagta aaactgatac cgccaaacgt
     2581 caccagaaaa ttgataacag agggcgttgc agcggggttg tcacttaagc gtatggtcaa
     2641 cctgacaacc cggtgtcctc aacggggaag gaataacccc gccatactta ccgccgcgcc
     2701 attttgcggg ttgccacaac cggaagcgca cggtcgaatt aaatttaacg acaccgtaca
     2761 gtgagacgaa cttcgccgtg cgctttcgtg ttgtgtgcct gcttttaacc acgtcaggcg
     2821 aggtggtatc cttaaaatca ccacagtttt aaggattcat taagcaatgt cgcaaccacc
     2881 aataaatccg cttaagaaca tgaaaattga ttactggtat aaagcgctta cagttgttgg
     2941 cgctgcgttg tttgtcttta atggaacgtc tttttttgac agatatcccg ttgttccatt
     3001 gggttttttg tcctccggca tcttttttat tggtttgggg gagtggatta atcaccctct
     3061 catagtgaga tttattggtc ctggagtttg gactcgtgga tataatcgtt cttcgtgcgc
     3121 actcggtatc atcttcgaca tacttggttg tttcctgatt gttacaggag tcgtcaagtt
     3181 cttctgatgt aaaaccgcaa atggggcacg taacgggaat tttgaaaagc gtttctccgg
     3241 gttccagaac aaaattttct gcggtctgat tttgcttctc atatttgtgc tccgcgtcat
     3301 tgtgagagca cattcttatt ctgagtgcct gtttaaactc actgaagctg agagcttctt
     3361 cgccttcggc aaggccttcg aagtattctt cgtaagcctt ttccatgatt gtgttgaaat
     3421 ccatatcact cacctgagtt tctttccagc cagcgacggg caccattttc ggttttaaac
     3481 gttttgcttt tggtatacgt catcgcggtg aacgtgccgt cctggttggg gaacacgcca
     3541 cataccagag attcgctgtt gccaagatcg atagtatcca tgctgacctc atttcccctt
     3601 aacgccgggg tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tggaggtaat
     3661 ttagttttct cattattttt cgtcaaggtt ttttgatgag aaaactcaag tgttggcgca
     3721 agataaagcc aatacattga aatgtaaggc tttaaaattt tgtgaaggga tgattattga
     3781 tgcttgttgc gtttgcgagc ctctaatagc tcggtgaaca ggcgattaaa attctcaacg
     3841 cgggcgcgga gttcgctgag ttgtgcttgt tgctctgatt taggaagtgc gcgatacaaa
     3901 cgcagcatct ctaactcatc ttccgataag tctaaggcac tatcgagtga aacaggggga
     3961 gctggtgttt tgtcctcgtc gccaaacagt atccaagttg gtgaacattg caatacctcg
     4021 gcgaggcgat gcaaattttg cccgcgcggg gctgtatggt cactttccca taatgaaatt
     4081 gatgagccag atacgccagc agctttgctt aaaccgtttt gacttaagcc tacctgctta
     4141 cgtctttctc taatgcgttg acctaaagtt ttctcgttca tatttagata tcttaataac
     4201 ccttgacttg agattccttg agtgattagc attgagaaaa ctcaatattg gaggtgcgat
     4261 gtttaaatca gacgtaatta atttttacgg tacgaaagcc aaagtagcga aagctgctgg
     4321 cgttgaccca tctgctgttt ctcaatggca agagctggtt cctgaaggtc gcgcgatgcg
     4381 tctacaggag gcatctggcg gcgagcttct gtatgatccc aaggtttatg acgaatatcg
     4441 taagacgaag cgggcggggc ggttgaacaa tgaaaatcac tcctgaacag gctcgtgagg
     4501 ctctggatgc ctggatatgt cgaccaggaa tgacacagga gcaggcgacg atattaatca
     4561 ctgaagcatt ctgggctttg aaagagcgcc cgaacatcga tgttcagcgt gtcacagatg
     4621 aaggtggcgc ggttgatcag cgagcacttg gcgttaatcg agtgaagata ttcgaacgct
     4681 ggaaggctat cgacaccagg gataagcgtg aaaagttcac ggcgctagtg cctgcaatta
     4741 tggaggctat tcggattaat gatttcaggt tgtatcgtga aattagtgac ggaaaaagca
     4801 tcacgtacat gatcgccggg ttaaacaaag aatatggcga tgtggtggag tccggactgc
     4861 tttttgctga tcctgccgta gtggatcgtg aaactgacga acttatagaa aaagcaattg
     4921 ctttcaaact tgcgtatcga cagcaatacc aacaaaaagc tggatggaat tatgagcctt
     4981 ctttttgctg aacgcccact ggttataaac acacagcttg cgatgaagat tggcttaaat
     5041 gaagccattg tgttgcagca gttgcattac tggttgagag ataccaattc cggcatggaa
     5101 tgtgatggtg ttcgctggat ttacaacaca acggaacaat ggctggaaca gttcccattc
     5161 tggtcagagt caacgttaaa gcgcgcgttt gcaagtctga aaacgctggg gcttttgcgt
     5221 tgtgaaaagc tcaataaatc aaagcgcgat atgaccaatt tctacacgat caactacggg
     5281 agcgagcttt tagatggtgg caaattgagc gaatccatcg gtttaaaatg cgccgctcca
     5341 tcaggtcaaa atgacacgat ggaagaggtc aaaatgaaac gctccattgg ttcaaaacga
     5401 cccaatgtca tcgggtcaaa atggcctgat gatcttacag agaatacaac agagattact
     5461 acagagaata aaaacacttt tcgtccggaa gcttcgcaac cggaccagca gaccgctgaa
     5521 caggattttt taacccggaa ccccgacgcg gttgtgttta gtgcgaaaaa acgccagtgg
     5581 ggtagcaggg aggatttggc gtgtgcgcag tggatttggg ggcggatcgt gaacctttac
     5641 gaacaggctg ccagcgacga tggagagatc atgcgaccaa aagagcctaa ctggacagcc
     5701 tgggccaatg acgtgcgcac aatgcggatg ctggatggca gaagccacag acaaatttgc
     5761 gaaatgtttg gtcgggtaca gcgagatcaa ttctgggtaa aaaacatcat gagcccgtca
     5821 aaactccgcg aaaaatggga cgaactggtc atccgcctgg gacgttcacc tgtacagcgt
     5881 tgtgtgaatc atatttctga accggataca gaaattccgc ctggtttcag gggataagtg
     5941 ttgattttag ggtataaagt aatgttcagg aggacttgtg gcaaaagttt ttacacaaga
     6001 agagcgggaa aaaattaaag ggcaggtggt ggaactcgta cgccagagtg ggcgcgagac
     6061 gttacgacaa ctggaagcta aaactggggc aacaagatat ctgatgagcg ttctggccag
     6121 agagctggtt gccagtggcg atgtatacag ctctggttac gggttattcc cgtctgaaca
     6181 ggcgcgtaag gactggcaaa acgcccgcaa aaaactctcg agggcaaagg tgaagaaacc
     6241 tgctgtggtt gatccggacc ttatctggtc attacctgac ggagaaatac gtcgctacga
     6301 cagtcgtcta aacataatct gtcgcgagtg ccggaaggca aggagaagca ggctattgtt
     6361 aacaccaagc tgtaatgtcc cctttgaacc attctaaaat gtccccagac aattctctgg
     6421 gggatttttc atgatcaaag agactgttac gatgagtcat aaggaactcc accgacttca
     6481 gattattcag gagtcaatta atcgacatat tactcaggaa caagctgcgg cacgcattgg
     6541 catttctatt cggcaggtta aacgtctggt gcaacggtat agaaatgaag ggccttctgg
     6601 tctggtttcc caccgacgtg gaaagcgtcc taataattcc ttttctactg aattcagagc
     6661 aacagtaatt tcactcctca aaggccgtta cgctgatttt ggacctacgt ttgcgtgcga
     6721 aaaattgcgc gagatacacg gtttatcttt atccgttgaa actctcagaa agtggatgat
     6781 agaagagggg ttatggcgtg aacgccgtcg taaaattgcc cgtatatatc aacgccgcat
     6841 gcgacgacca tcttacggtg aactgatcca gattgatggc tcacctcatg actggtttga
     6901 aaatcgaggc cccagatgta cactgatcgt tttcattgat gatgccacca gtgcgttgat
     6961 ggcgttgcgt tttgtgcctg ctgaaacaac ccgggcttac atggaaaccc tccggggtta
     7021 ccttaatgat catggcgtac cgctcgctct ctactctgat agacacagta tattcagggt
     7081 aaataaccca gagcgggaag gtgagctgac ccagttcact cgtgcgataa agacactggg
     7141 catcgagcca atccatgcca acagcccgca ggcaaaaggg cgggtagagc gcgccaatca
     7201 gacactacag gacaggctgg tcaaagaaat gcggcttcag aatatcagtg atattgaaac
     7261 agcaaatgca tggttgccga cctttattga agcctataac aaccggttcg ctacgtcgcc
     7321 tcgtactact gataatgctc atcttgatgt gcaccattct gaagaggaac tgggttatat
     7381 cttcagccta caggcgaagc gcgttctgtc taaaaatctc actttccagt acaaaagcag
     7441 tgcgtttcag gtacgcagtg agggccgggg atatcgactt aggcattcgg ttgttactgt
     7501 atgtgagaac tttgacggtg aaattaacgt tctgtatgac gggaaagcgc tgggctggga
     7561 aaagtatgtt gatggcccgg agcctatacc actggatgat gaaaagagtg tccatgaacg
     7621 agtggataat gcccgtattg atttacgctc aaaatactat gttaaatcta aagctgacca
     7681 tccctggctt acgcgccgaa cgcaaagtca tcagcaagtt aagcccccga agttacctaa
     7741 aaagaagcct gatcccgata aaatagattg aaaccaagat cgattcggtt gagtgcatat
     7801 ccattcatag ggtagattct taagtcgcgt ttctgatgtt cattttcggg tggtttgtta
     7861 cttgttttac cggggatatg ccagaaacgc gctgagtcag tctgggcggt gcgcgtaatg
     7921 aggcgttatg gtaaatagcc tatgctaatg tccgctaaga gcaagaagcg gaagttggca
     7981 gttttgtgga ctgtccccac aaaagtgact acagaaatag ttgcaattca taattgatca
     8041 tgggttgtca gttaaactcg tggcgattta aatagactaa ttgggagtgc gtccattact
     8101 tatatcttgt aatgttaact atcagaaatg atacaaagat aatatgtctt taaagaaaag
     8161 gctgatggcg aaaagtggcc cgatgagggc cacaatacgg ctgtcactta gacgtaaata
     8221 tcaatggtgc cagcggtatt tgtatcgtct tttttctctt cttttttatc aggctgaact
     8281 gtcgcgtctt tattcttttt ctctgcctgc tgccttaaca actgctccag ttgagccctg
     8341 aggctttcaa tttgcttctg taccaatgca gccatttctt ttttctgctg tgtcgtcatc
     8401 ccctcttccg atgagatttt cccaagcttt tcagtcagca cctgaatttg tcttgtgatt
     8461 ttggctattt ctgatgttcc ttccggggcg gagttgtttg aaataacggt tgaggtattt
     8521 ccctgaattg tgacagacat agatttctcc ttttaaaaaa gcactatcgg catgcacaaa
     8581 aaaatcttta atcgtatttc ttgtgtcatt aattgtttga tgttcagatt gttttcctcg
     8641 cgggctggcg cgcctcagaa agtaaagctt gttgacaggg gtaaacgttc ggcaataatt
     8701 ttctgccgca tgcgggtgtt gcataaaacg tgctacgttc ctttatcgac aggtcaggtc
     8761 accgctcacc cgccgacgag aaagcaacac tgacatgcta aagcaaaaaa tagatgaata
     8821 agttgagttg tgcatatgta gcctgaccgt cacaaagtat atggtgtctg taccagtaag
     8881 atgatggccg gactctttaa aaacgagctg acctgcacaa tacaggatgg acttagcaat
     8941 ggctgctcct ggcacaaagc ggacagtgat caccgttctt acgactactt tctgacttcc
     9001 ttcgtgactt gccctaagca tgttgtagtg cgatacttgt aatgacattt gtaattacaa
     9061 gaggtgtaag acatgggtag cattaacctg cgtattgacg atgaacttaa agcgcgttct
     9121 tacgccgcgc ttgaaaaaat gggtgtaact ccttctgaag cgcttcgtct catgctcgag
     9181 tatatcgctg acaatgaacg cttgccgttc aaacagacac tcctgagtga tgaagatgct
     9241 gaacttgtgg agatagtgaa agaacggctt cgtaatccta agccagtacg tgtgacgctg
     9301 gatgaactct gatggcgtat tttctggatt ttgacgagcg ggcactaaag gaatggcgaa
     9361 agctgggctc gacggtacgt gaacagttga aaaagaagct ggttgaagta cttgagtcac
     9421 cccggattga agcaaacaag ctccgtggta tgcctgattg ttacaagatt aagctccggt
     9481 cttcaggcta tcgccttgta taccaggtta tagacgagaa agttgtcgtt ttcgtgattt
     9541 ctgttgggaa aagagaacgc tcggaagtat atagcgaggc ggtcaaacgc attctctgaa
     9601 ccaaagcatg acatctctgt ttcgcaccga aggtgacact tctgctttgc gttgacagga
     9661 gaagcaggct atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac
     9721 cgtcatagtg acggcactgg taacgaggaa agacctctgc gaggtacgaa tccgaaccgg
     9781 ccagacggag gtcgctgtct tcacagctta cgaacctgag gagtaagaga cccggcgggg
     9841 gagaaatccc tcgccacctc tgatgtggca ggcatcctca acgcacccgc acttaacccg
     9901 cttcggcggg tttttgtttt tattttcaac gcgtttgaag ttctggacgg tgccggaata
     9961 gaatcaaaaa tacttaagta gcgcgcaggg ataagaggga tggtccctta aaggggagag
    10021 ctaattatcc ggaaggattc tgatgatgaa catcgaagaa ctgcgtaaaa ttttttgtga
    10081 agatggcctc tatgctgtgt gcgttgaaaa tggaaatctt gttagtcatt accgcattat
    10141 gtgtttgcga aagaatgggg ctgcgttaat taattttgtg gatgctcggg tcacggacgg
    10201 atttatcttg cgcgaaggtg agtttgtcac ttcattacag gcattgaaag agatcggaat
    10261 aaaagctggc ttttctgctt tttcaggaga ataaactcat ctacaatctt gcgcggggct
    10321 gaactcccgc tgagtaacac cgtgccaccg gagaaaaccg atggcacgca acgcaaaata
    10381 ttacaattct gataattcgc ccgttcttgc ctgcacgcac gggcggtatt ctcacgcatt
    10441 caagtctgaa tggttccagc accctccatg cactgcagaa caggccgaat ggctgattca
    10501 ttcttaccgc aggcgcgggt tcgaggttaa gaaagctctc agtctcgact atcggcactg
    10561 gataatctct gtcaggctgc cttattccga acgcccacca cgtgcgtccc gcactttcca
    10621 gcaacggatc tggaggtaac gtgcgggtat tacttagacc tgttctggtg cctgagcttg
    10681 ggctggtggt ccttaagccg ggccgtgaat ccatacagat atttcataat cctcgagtgc
    10741 tggtggaacc ggaaccaaaa agcatgcgta atctgccatc cggagtcgtt cctgccgttc
    10801 gccagccgct ggcggaagac aaaacattgc tgccgttttt tagtaacgaa cgggtgattc
    10861 gtgctgctgg cggcgttggc gcattgtccg actggctatt acgtcatgtt acatcctgcc
    10921 agtggcctaa tggcgattac catcacactg aaacagtcat tcaccgttat ggtaccggcg
    10981 caatggtgtt gtgctggcac tgcgacaacc aactgcgtga ccagacatcg gaatcactgg
    11041 agctgcttgc tcaacaaaat ctgacagcat gggtgattga cgtcatccgt cacgcaataa
    11101 gcggtacgca ggagcgggaa ttatctttgg ctgaattatc ctggtgggcg gtctgcaatc
    11161 aggtggtgga tgcactacct gaggctgtat cgcgtcgttc gctgggatta ccagcggaaa
    11221 aaatctgctc ggtgtaccgc gaaagcgaca tcgtaccggg agagcagacc gccaccagca
    11281 tattgaaaca acgcacaaaa aatcttgcac cgttgcctta cgcccaccag caacaaaaat
    11341 caccacagga aaagacggtg gtaagcatca ccgttgatcc agagtctccg gaatctttca
    11401 tgaagctgcc taaacgtcgc cgctgggtta aggagaaata cacacgttgg gttaagacac
    11461 agccgtgtgc ttgctgcggt atgccagccg acgatccgca tcatctgatt ggtcacgggc
    11521 agggcggaat gggaacaaaa gcacatgatc tctttgtgtt gcctttgtgc agaaagcatc
    11581 acaacgagct gcatacggat acagtggcat ttgaagataa gtatggctcc caactggagc
    11641 tgatatttcg ttttatcgat cgcgcgctgg caattggcgt actggcgtaa gtggagaacg
    11701 agcatgaacc ttgaagcctt accaaaatat tactccccaa aatctccaaa attgagcgat
    11761 gacgctccag cgacaggcac cggttgttta acaattacgg atgtaatggc agcgcagggg
    11821 atggtgcagt cgaaagcacc acttgggttg gccttatttc tggcaaaagt tggtgttcag
    11881 gaccctcagt ttgcgattga aggcctgcta aattacgcga tggcactgga taacccgaca
    11941 ttgaacaaat tgagtgaaga aatccggtta cagattattc cttacctcgt gaattttgcc
    12001 tttgctgatt actccaggtc tgcggcaagt aaggctcgct gtgagcattg ttcaggtacg
    12061 ggattttata atgtattgcg cgaagtggtg aaacactacc gacgcgggga atctgtaatc
    12121 aaggaagaat gggtgaagga actatgtcag cattgccatg gtaagggtga agtcagcaca
    12181 gcgtgcagag ggtgtaaggg taaagggatt gttctggatg aaaaaagaac ccggtttcat
    12241 ggcgtaccgg tatataagat ttgtgggcgt tgtaatggaa accggtttag tcgtttaccg
    12301 accacgctgg cacgacgtca tgtccagaag ctggtaccag acctgaccga ttatcagtgg
    12361 tataaggggt atgcggacgt cattggtaaa ctgggtaaca aagtgctggc aggaagaagc
    12421 atacgcggaa gcgcaattga ggaaggtgac gagataaatg atttttgctg aagatggcga
    12481 catgatgttt gcatttttca aaaaatatgg ataaaatttt ttcaacgatg ggctttgtat
    12541 acccgacgtt aagaaaaagt agaaaacccg ctgatgcgcg ggttttgtgc tttaaatggg
    12601 gcaatggtaa tgttgaatct catcccggga ctcatgtctg ttaacttatt atttagctgg
    12661 tgacttggtt atttgcctga tgtttaaaat gttttcttcc agtacaatgt ccctaaacac
    12721 aatgagtctg cttattatat tattagcaga gctattacgg ccaaagtaca gcataagctt
    12781 ttaaagccaa tcaaccagtc atcaagacag acggggttat tcataaaaac tctccatgtg
    12841 tgatccgatg gggcctgaaa ttaaagcttt aatatagctc atgaaaggta aacattggca
    12901 gctgaagggc cacgcagacc atttatccgg caaaattcca cgcgtaatcc ggtggtaatt
    12961 tcttctgcat cgcggagatt gagcgctgaa acatgaagct ggacatcgat acgaccatcg
    13021 gatggggtga taagaccctt gccgcttttg ccgtcaaagg ttttgacaat tcctgtcatt
    13081 ttacgggaca aaaaaattcc ttaatactga taacttggcg cactatacac acgttcctga
    13141 agaaagctat agttttttga tggggttgaa gatggctgga tgtctaaaat aaacattgct
    13201 tcatatgttc aactatgcgt taatgattgc gtcggtttga agaacagacg atatacgaag
    13261 tagtttacta aagcagttct catttcaggt gttattcact tattccttct ttgagtctct
    13321 ccaattaagt acgaagtcgt ttctgttatg caaaccattt atgccgaaag gctcaagtta
    13381 aggaatgtag aatgtcaaat aaaatgactg gtttagtaaa atggtttaac gctgataaag
    13441 gtttcggctt tatttctcct gttgatggta gtaaagatgt gtttgtgcat ttttctgcga
    13501 ttcagaatga taattatcga accttatttg aaggtcaaaa ggttaccttc tctatagaga
    13561 gaggtgctaa aggtcctgca gcagcaaatg tcatcattac tgattaaaat tcatcgctcg
    13621 tctgtatacg ataacgaaga aggctgatgc ctgagtagag atacggacag agtagtgaat
    13681 attggatctc tttaataaaa agtaaggagg tccaatacat gaaacaatgg ctagcatatt
    13741 tggcaaaatc ttaatcagga aaagtatgct aaccattgtg gtgaagtgca ggtttgctgc
    13801 atgaatagtt tcacagcaga agctaactgc tggcatcgca aaacaaagtg cgtaagtgga
    13861 tgactcccac aaaaagcacc acaatctcaa acccgctcag gcgggt
//
LOCUS       DAZFKM010000133        13761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFKM010000133 DAZFKM010000000
VERSION     DAZFKM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            461..643
                     /locus_tag="ACHKAY_004059"
     CDS             461..643
                     /locus_tag="ACHKAY_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702638.1"
                     /translation="MKWYTPEHVVEAFKKGELTRHQVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
     gene            791..1456
                     /locus_tag="ACHKAY_004060"
     CDS             791..1456
                     /locus_tag="ACHKAY_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5702639.1"
                     /translation="MNQNTKTIAEAKAQCLNKKDSKQTYVVVEQWLKMFKKWFSPSGK
                     EYTATLPAVEIYSYVAGFNEQGQECYANQKTLANIGMVSDRQVRNIINMFIDMGVLSV
                     EARDGQTSLYKALPFTDAHITPPEDVKTSVNVEQPAPETQEPLEATHSIEQETQAEIT
                     EQSDLFTWLISIDSQKPMQEETENFIKRALTSKRIILEDDAFNALMIALRKESEIYDS
                     IPF"
     gene            1469..1726
                     /locus_tag="ACHKAY_004061"
     CDS             1469..1726
                     /locus_tag="ACHKAY_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702640.1"
                     /translation="MKYNLTLNKRTFEITESGYTLAKQLADSNYELPAIISFQELPHS
                     KEHTQHWLNHLSHFGVIKYEAVNSQTFRLLEISPFELWVAL"
     gene            1723..1929
                     /locus_tag="ACHKAY_004062"
     CDS             1723..1929
                     /locus_tag="ACHKAY_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702641.1"
                     /translation="MTIFDSEFSQETFLQYLRERTGMILFDSEFSGVVGEQFLYALLD
                     SLSKQGLLQYTKTHMKAFRIDSVA"
     gene            2051..2431
                     /locus_tag="ACHKAY_004063"
     CDS             2051..2431
                     /locus_tag="ACHKAY_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702642.1"
                     /translation="MSKKKGKKNVITTIGEACDQYALNNPRKYNYEHDAQPGEELIFI
                     LEEPSPELTDEELSLIQSMDGVYFNPRPRKTTVIDSSLLKRWQCLGFQDMHHFRGYMA
                     LNSLQYAGVRETELLLLDKIKIDD"
     gene            2473..2694
                     /locus_tag="ACHKAY_004064"
     CDS             2473..2694
                     /locus_tag="ACHKAY_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702643.1"
                     /translation="MINKAILETTTFHDLKLLSECFISLIERAQEENDLGLLDDIIDN
                     IATYKIPLTIAMKKAREAQQQLEQSEEVA"
     gene            2694..3002
                     /locus_tag="ACHKAY_004065"
     CDS             2694..3002
                     /locus_tag="ACHKAY_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702644.1"
                     /translation="MSEVHNVTIQMMDLVIDLQKAVNAALTFPSELTITQAVINLEST
                     REALQLLIAACEESEGAQIEADDLSNLIIVIHDTTEIMKEAVADLQPLINELIDKANN
                     "
     gene            3002..3211
                     /locus_tag="ACHKAY_004066"
     CDS             3002..3211
                     /locus_tag="ACHKAY_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702645.1"
                     /translation="MTKTKRVRLLALTEEGKAGNCFTVGKVYTGLLDTYTRLMHVTDD
                     NGNDQTIVLDGNCVFGEWELTTEKS"
     gene            4090..4305
                     /locus_tag="ACHKAY_004067"
     CDS             4090..4305
                     /locus_tag="ACHKAY_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702646.1"
                     /translation="MQNLILKNGNEELVITDETFNATAYMLGITPTELKKHCDKLFKS
                     ADEQKPTMQDLIKAKAEQIKQSKKGNY"
     gene            4308..4679
                     /locus_tag="ACHKAY_004068"
     CDS             4308..4679
                     /locus_tag="ACHKAY_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702647.1"
                     /translation="MAIINMSDLFPVRKSFTDIVSYATDEFGCVQSFTVDLAVDVECG
                     DLITSDGAEYVSGGDVLVVVSDAVSAGDAKTVNVLRSNAGLVCIKSDALNVEGAVKEA
                     AIAALAAKGFQMAGFNTVFTS"
     gene            4698..5726
                     /locus_tag="ACHKAY_004069"
     CDS             4698..5726
                     /locus_tag="ACHKAY_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5702648.1"
                     /translation="MIIGNESVDLAPLFSLHDSRNFLLTSLDFTDPVGVSSHKVAVSQ
                     LVETNESLFNKETSRFSSEHNVTKREQGKEFLVEIPYFLREDMIKPQDVQGKRKPGAT
                     VEETLTDIYAEYVAKHHIAFQRTKESVLAASLFSGRTYTPKTDDVLIQWGDLFSVVPM
                     TATIDASSTTPSMVFQQFDQMHTDVITKAQSQAAAVERIVVFCKPAAFNAIRYSAGMV
                     NAFQYASPTDDANIVYSVRSLLPGVTTFTIPGTNIDIVKVTDPLLLAHMTSDAVAVPK
                     FAKGSNVYQNIFGAPSSTFDLLNSGAAEYYSYAFSPARNDAVNVVTENSQMVVNHGVG
                     FTVQITVA"
     gene            5880..6158
                     /locus_tag="ACHKAY_004070"
     CDS             5880..6158
                     /locus_tag="ACHKAY_004070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702649.1"
                     /translation="MSEQNNNPVEILKAVSTLPEDILNISQVCKFLGISNTAVRQRIF
                     TTGCSAMEAVQYYVTKQNKTKQNKTKQIKGKGKYYGFKHLNLNKQGCI"
     gene            6115..7956
                     /locus_tag="ACHKAY_004071"
     CDS             6115..7956
                     /locus_tag="ACHKAY_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702650.1"
                     /translation="MDLNISTLTNKVVFKVDFASLQKARDAGKKFQKEMENLTNPTLK
                     GKKAKRAIDEAHDLVKARKAAQAKTDAKASDKQAQTDKKNADKVAKAEAKAKAKAAIA
                     ADKQIRAKATIAEKAEIKRQQAFRQLEGIKKGRMGDGMDSAQYYKTLQYIKAQTAAYE
                     KGAISQAKMNHLIRDRITLERRAAAVKARQVAADGGKYPHMAKQLKGGHIGGGVGAMV
                     GGAALTAATLGGAAIFNTVTDQASKITDTNRKAKAVNVNTNALLALNQYGLEHGVDSS
                     VDQRQDNLKDVQERLGESVGNTKWDAKKGRWAGGNGAVDNILNKYGWSPEQIKGFQTD
                     PLGFLGAVVKEGERRGLSDQEQGHILEDLGNDLQYFRGAFSRNGEELNKTIVKMQKAN
                     ATYTQADLDAAERLQAVQANMNMAWEGSKKAIMTGFGEEFDAQAGEEWAQMLKDLNPL
                     FKELGSSTAEMMSAFAGAVSWLTAAYQNINPENKHENLDSSGMYYKDSFVGGMVDWGR
                     SALGLSPIPGTEDKPTTGGTAIQNYSSPSDNVQSLKENVSRDLNTTPSMTYAPSIVVN
                     VPESKVDVNIAPDGSQLGSYLDYKVGSIQQDFERSFTLAALSGQAPS"
     gene            complement(8064..9284)
                     /locus_tag="ACHKAY_004072"
     CDS             complement(8064..9284)
                     /locus_tag="ACHKAY_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5702651.1"
                     /translation="MSIKSQAYLFKRKDGIWMFQIFVPKYLRHLYPYKLWRKSTSTRD
                     LNEARRFRNHLLLEWEELKRKHNPDNPERRLQQAIESLHSEARANKNTQRAVKRGYAS
                     PSLCLIRDEYAECYRERRSYSTLQKSARAVELFLQSIQKDDIHIEKIGRRLVTVFLDD
                     QKQRGAADQSRQNWLTCLGSLYEFAKRRYDGIPDSNPFHGHNLEARRTIESYQPFTPE
                     QMQKLLDAAEPEIRNIILMGLFSGCRLDELASLKKSEVVTVEGIRCFYISKSKTKAGI
                     RHVPIHSRLSIIVDEYLNHNYGEYLFPHSNKVKRIDGKKGPFYSQAFSRLRRSVLPIA
                     TNRQCFHSLRGMFITCLDRAGVPEQRIGAITGHTEQRAKTEAFRTYSQGASIPELAEY
                     VELVRYELNIDFKA"
     gene            9526..11133
                     /locus_tag="ACHKAY_004073"
     CDS             9526..11133
                     /locus_tag="ACHKAY_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5702652.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            11126..11785
                     /locus_tag="ACHKAY_004074"
     CDS             11126..11785
                     /locus_tag="ACHKAY_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO5702653.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            12694..13422
                     /locus_tag="ACHKAY_004075"
     CDS             12694..13422
                     /locus_tag="ACHKAY_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5702654.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            13424..13624
                     /locus_tag="ACHKAY_004076"
     CDS             13424..13624
                     /locus_tag="ACHKAY_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702655.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
BASE COUNT         4072 a         3013 c         3083 g         3593 t
ORIGIN      
        1 ttagcggtaa tagattagct cgttatagag cttctgagga gctttacagg tattttataa
       61 gatgtctgaa atcagagttt atctatccac catctttcca tcatctgata aatcaaaaat
      121 aagcccctta aataagcctc tgacgagata aacaacctct acataaggga atgtattgtt
      181 tttatcaaaa cccccgtcag agtgcgattg agagcgtttt aggcaacagg gaagaattgt
      241 accagagtag gcagcacaaa aagctaagaa aaattataag aaattgaaaa tgatcaagtt
      301 ttttataaga gagggttgac aaagccgccg ttatgtagta ttgtaagtag ggaggggaga
      361 aacatctctc tccatctaaa taatcaaatc acagaacact gcactttgag ccgtcaggct
      421 gcaaggtgtg tttttctttt atctaaataa ggagtttttt atgaaatggt atacaccgga
      481 acacgtcgta gaggcattta aaaaaggtga gctaacacga catcaagtaa ggatgaacag
      541 gaacacagcg cggcgctgtg gttatccaga gcgtgaaaaa tgctttgacg atgcactaaa
      601 aattattgat gaactgcgta aggcagaagc agagaaagaa taataacagg agaaggagaa
      661 aaccaaatga atattaattt tgattctgca acttataacg cgttgttaaa tcgtgcaaag
      721 gcagaaggga aatcaattcc aaaacttgtt aatgaattac taattacagt ccttacaaag
      781 gaggaactaa atgaaccaaa acaccaaaac aattgccgaa gcaaaagcgc aatgcctcaa
      841 taagaaagat agtaaacaaa catatgtagt agttgaacaa tggctgaaga tgtttaaaaa
      901 gtggttctca ccgtcaggta aggaatacac cgcgacactt ccagccgttg aaatctacag
      961 ttacgtagct gggttcaatg aacaagggca agaatgttac gccaatcaaa aaacattagc
     1021 taatattgga atggttagtg atcgtcaggt gcgtaatatt ataaatatgt ttattgatat
     1081 gggtgttctt tctgtagagg cacgggacgg gcaaacatca ctctataaag cattaccctt
     1141 tacagatgca cacatcacac caccggaaga cgtaaaaacc tctgtaaacg tagaacagcc
     1201 tgcacctgaa acacaggaac cgttagaagc gacacactct attgagcagg aaacacaggc
     1261 agaaatcacg gaacaatctg atctttttac atggcttatt tccatagaca gccaaaagcc
     1321 tatgcaagaa gaaacagaaa attttataaa acgtgcactg acctcgaaga ggataattct
     1381 tgaagatgat gcttttaatg cactgatgat agccctgaga aaagaaagtg aaatctatga
     1441 ctcaatacca ttttaaggag aataaaaaat gaaatacaac ctgacattaa acaaacgaac
     1501 attcgaaatt acagaaagcg gctacacact ggcaaaacag cttgctgata gcaattatga
     1561 gctacccgcg attatcagct ttcaggagtt acctcatagt aaagaacaca cacagcactg
     1621 gttaaatcac ctgagccatt ttggggtaat taagtatgag gctgtgaata gtcagacgtt
     1681 ccgtttgctt gagataagcc ctttcgaatt gtgggtggcg ctgtgaccat attcgatagc
     1741 gaattttcac aagaaacctt tctgcaatat ttacgggaaa gaacgggaat gattctgttt
     1801 gattctgagt tttccggtgt ggtaggtgag caatttttat atgctcttct cgatagccta
     1861 agtaaacaag gattattgca gtataccaaa actcatatga aagcattcag aatcgactct
     1921 gtagcttaaa acaaacaact atcaaacatc ttgaagggtg aaacaatgga aagcttaata
     1981 tttttcttag tatttatgat cgcttccttt tttaacactg caacaagaaa aacaaaacga
     2041 aggggaagca atgagcaaga aaaaaggcaa gaaaaacgta ataacaacta ttggcgaagc
     2101 atgtgatcag tatgcgttaa acaatcctcg caaatataat tatgagcatg atgcacaacc
     2161 aggcgaggaa ttgatcttta tacttgaaga accaagccca gaattaacag acgaagaatt
     2221 atcactcatt caaagtatgg acggtgttta ctttaatccg cgcccacgca aaacaacggt
     2281 tatagattcc agtcttctga aaagatggca gtgtttgggt tttcaggata tgcaccactt
     2341 tagggggtat atggctctta acagtctgca atatgcaggg gtgagagaaa ccgaattact
     2401 tctgcttgat aaaataaaaa tcgacgatta acagcgtttg ctgaatataa cccttaacaa
     2461 aaggaaaata aaatgattaa caaagcaatt ttagagacaa ccacttttca cgatcttaaa
     2521 cttctttctg aatgcttcat aagcctgatt gaacgtgcac aggaagaaaa cgatctcggt
     2581 ttactcgatg atattataga caacattgca acttataaaa tcccgctcac tattgctatg
     2641 aagaaagctc gtgaagcaca acaacaatta gaacaatcag aggaagtggc ataatgagcg
     2701 aagtacacaa cgtaactatt caaatgatgg atctggttat tgatttacag aaagcagtaa
     2761 acgcggctct gacttttcca agcgagctaa ccatcacaca ggcggttatt aatctggaat
     2821 cgacgcggga agcactgcaa ctacttattg cagcgtgcga agagagcgaa ggtgcacaga
     2881 ttgaagctga tgacctttct aatctgatca tcgtcattca tgacaccact gaaatcatga
     2941 aagaagcggt agcagatctg caacccctca ttaatgaatt aatcgataag gctaacaact
     3001 aatgacaaaa actaaacgtg tgcgccttct ggcgctcaca gaagaaggga aagcgggtaa
     3061 ttgtttcaca gtaggtaaag tgtataccgg cttactcgac acttacaccc gcctgatgca
     3121 cgtaaccgat gataacggta acgatcaaac aatcgtttta gatggtaatt gcgtatttgg
     3181 tgagtgggaa ctgacaaccg aaaaaagttg aaaataattg catttaccta ttgactttta
     3241 ctaacagaaa tgttagaata ataaatgtgg gtttttgaat cgaaaatatg tgttagctgg
     3301 cgttaatact tctcccgatt ctaatcctac acgaaagcgg ggtagacctc aagggggcgc
     3361 gccctatcta acctatcccg ctttcccttt acagtgattt tttagaaatt gtttccctcg
     3421 ttaatagttt cattttattt tccttattgc tttatcaaaa ttgtgtctta agaatgcagg
     3481 ggctacggat ttggcccctc atctttttaa ccaactgcga agcatgaaac actttctaaa
     3541 aacactgttt acagtgtcta taaaccccgt ataaacaaat aacggtgtat atctgtttct
     3601 tgacaacagt tttctactaa gcttaaagct gaatatgttt ttgtttgttg gtgtttatta
     3661 aggtgccaat cttagaacgc gaaagcgtgg atgatccccg aaatcatctc ggcaccgcct
     3721 cctattaaaa tgagaatcca aaaggtttga aagttaatag gtgattcctg aaagtctgat
     3781 cgatgcatcc acattgtgaa agacagcgat tagccgtcgc acgaataaca ggtttataga
     3841 ttcctctcca aagggggcga cctcctaagc gcctactccc tgtaagcctg ttattcaccc
     3901 ctcaagggta tataacaata agcaatctta ttcttatatg tcctcctctt taagttcata
     3961 taaactgcga tttagtaaca ctctataaca tccttcaaat gttgtattca taaagccgga
     4021 agctttcccc ttccggtttt atttttatca gcactaaaca agactgttag gaataaaggt
     4081 aaagcaataa tgcaaaatct cattctaaag aatggtaacg aagaattggt tatcactgat
     4141 gaaacattca atgctacggc ttatatgctg gggatcactc ccacagaatt gaaaaaacat
     4201 tgtgataagc tttttaagtc tgctgatgaa cagaaaccta ctatgcagga tctgattaaa
     4261 gcaaaagcag aacaaatcaa acaatccaaa aaaggaaact attaattatg gctattatta
     4321 atatgagcga tctgtttcca gttcgcaaat catttaccga tatcgtttca tatgcaactg
     4381 atgagttcgg ttgtgtgcaa tcttttaccg ttgatctggc ggttgatgtt gaatgcggcg
     4441 atctgattac cagtgacggc gctgaatatg taagcggcgg cgatgttctt gtagttgtgt
     4501 ctgatgcggt atccgctggc gacgctaaaa ccgttaacgt gctgcgtagc aatgccggac
     4561 tggtttgcat caagtccgat gctctgaatg ttgaaggcgc tgtgaaagaa gctgctattg
     4621 ctgccctggc tgcaaaaggt ttccagatgg ctggttttaa cactgtattc acatcttaat
     4681 ataaaggaat aaaaataatg attattggta atgaatccgt cgatctggct ccgctgttta
     4741 gtctgcacga ctctcgcaac ttcctgttaa cttctctcga ctttactgat ccggtaggtg
     4801 tgagttctca taaagtggct gttagccaac tggtggaaac taacgaatct ctgttcaaca
     4861 aagaaacctc tcgtttctct tctgaacata acgtgactaa acgtgaacag ggtaaagaat
     4921 ttttagtaga gatcccctac ttcctgcgtg aagatatgat taagccgcaa gacgtgcaag
     4981 gcaaacgtaa accaggcgct accgttgaag aaactctgac agatatctac gcggaatatg
     5041 ttgctaagca ccacattgct ttccagcgta ctaaagaaag cgtgctggct gcttccctgt
     5101 tctctggtcg cacttacacc cctaaaactg atgatgtgct gatccagtgg ggcgatctgt
     5161 ttagtgttgt tccgatgact gcaaccatcg acgcatcaag caccacgcct tctatggtgt
     5221 tccagcaatt cgatcagatg catacagacg ttattactaa agcacaaagc caggcggctg
     5281 ctgtagaacg tatcgttgta ttctgtaaac ctgctgcttt caacgctatc cgttacagtg
     5341 ctggcatggt taacgcgttc cagtatgcaa gccctaccga tgacgcaaac atcgtttata
     5401 gcgtgcgctc cctgctgcca ggtgtgacta ccttcactat tcctgggact aacatcgata
     5461 tcgttaaagt tactgatccg ctgctgctgg ctcatatgac ttctgatgcg gttgctgttc
     5521 cgaagttcgc taaaggctct aacgtctatc agaacatttt cggcgctcct tcttcaacgt
     5581 tcgacctgct gaattctggc gcggctgaat attacagcta tgcgtttagc ccagcacgta
     5641 acgacgctgt aaacgtagtt actgagaata gccagatggt tgtaaaccac ggcgtaggct
     5701 tcactgtaca gatcacagta gcgtaataaa gcgttaatag tcgtttcctg tgcgactgga
     5761 aataaaacag gcaataaggg caaaagggat tattaaacct gtagcgcaaa tacagtaaac
     5821 aatctctttt gccctttttt cgtttatacc gtctattaaa atattgtgag agaaaaatca
     5881 tgagcgaaca aaacaacaat ccagttgaga tcctgaaagc tgtttccacc ttaccggaag
     5941 atattctgaa tatctctcaa gtgtgtaagt ttttagggat ctctaatact gctgtacgtc
     6001 aaagaatctt cactactggc tgctcagcta tggaagcagt gcaatactac gtaacaaaac
     6061 aaaacaaaac aaaacaaaac aaaacaaaac aaataaaagg taaaggaaaa tattatggat
     6121 ttaaacatct caaccttaac aaacaaggtt gtatttaaag ttgattttgc atcattacag
     6181 aaagcaaggg acgcagggaa gaaatttcag aaagaaatgg aaaacctaac caatccaaca
     6241 ttgaaaggga agaaggctaa acgagctata gacgaggctc atgatctggt aaaggctcgt
     6301 aaagctgcac aggctaaaac tgatgccaaa gcgtcagaca agcaagctca aaccgataag
     6361 aagaacgctg ataaggtggc aaaggcagaa gctaaagcga aggctaaagc cgctatagct
     6421 gccgataaac agatccgcgc taaagcgaca atagcagaga aagcagagat taagcgccag
     6481 caagctttca gacagcttga agggattaag aaagggcgta tgggcgacgg tatggactcc
     6541 gcccaatact acaaaacgtt gcaatacatc aaagcacaaa cggcagcgta tgagaaaggc
     6601 gctattagtc aggctaagat gaatcacctg atccgtgatc gtatcacgtt agaacgccgt
     6661 gcggctgctg taaaagctcg ccaggtggcg gctgatggcg gtaaatatcc acatatggct
     6721 aaacagctta aaggcggtca tataggcggt ggtgtaggtg caatggtggg cggtgccgct
     6781 ctcactgctg ccactctcgg cggtgctgcg atattcaata ccgtaaccga tcaggcaagc
     6841 aagatcacag acactaaccg caaggcaaag gctgtcaacg taaacactaa cgccctgtta
     6901 gcacttaatc agtacggctt agaacacggt gtcgatagtt ccgtagacca acgtcaagac
     6961 aaccttaaag acgttcaaga gcgtttaggt gaatccgtag ggaataccaa atgggacgct
     7021 aagaaaggtc gctgggctgg cggtaatggt gccgtcgata acatccttaa taaatatggt
     7081 tggagtcctg aacagattaa aggtttccag actgatccat taggtttcct gggcgctgta
     7141 gtgaaagaag gagaacgccg tggactctct gatcaggagc agggtcacat ccttgaagac
     7201 cttggaaatg atttacaata tttccgtggt gctttcagtc gtaacggtga agagctaaat
     7261 aaaaccatcg ttaagatgca aaaagccaat gccacctata cgcaagcgga tcttgacgct
     7321 gctgaacgtc tgcaagctgt acaggctaat atgaatatgg cctgggaagg tagtaagaaa
     7381 gctatcatga cgggattcgg tgaagagttc gacgctcagg ctggtgaaga gtgggcgcaa
     7441 atgttgaaag atctcaatcc tctgtttaaa gaacttggaa gctcaacggc tgaaatgatg
     7501 agcgcctttg ctggtgctgt atcgtggctt actgctgcat accagaatat aaatccagag
     7561 aacaaacatg aaaaccttga ttcgtccggt atgtattata aggattcgtt tgtaggcggc
     7621 atggttgatt ggggtcgtag tgctttaggc ttatcgccta tccctggcac ggaagacaag
     7681 ccaacgactg gcggaactgc aattcagaac tattcaagcc cgtccgataa cgtgcaatcc
     7741 ctgaaagaga acgttagccg tgatctgaac actaccccat ctatgacgta tgcgccaagc
     7801 attgtcgtaa acgtgccaga aagtaaggtt gatgtgaata tcgctcctga tggtagtcag
     7861 cttggatcat acctcgatta caaagtggga agtatccaac aagattttga acgtagtttc
     7921 acgttggcgg ctctgtctgg gcaagcacca agttaatgat aaagagaatg tttaatcacc
     7981 atttaaaaca ctcttataag gaattaagca tataagaggc tcccattaca aggaagcctc
     8041 tttattgcgc ctatggcgtt gttttaagcc ttaaaatcaa tatttaattc atatcttact
     8101 aattctacat attcagctaa ttctggaatg cttgcccctt gtgagtaggt gcgaaaggct
     8161 tctgttttag ctctctgctc tgtatgtccg gtaatggcac cgatccgctg ttcaggcact
     8221 ccggcacgat caagacaagt gataaacatc ccgcgcaagc tgtgaaagca ttgtctattt
     8281 gtagcgattg gcaagacgct acgacgtaaa cgtgagaacg cttgagaata gaacgggcct
     8341 ttcttaccgt cgattctttt tactttgttc gagtgaggga acaaatattc accataatta
     8401 tgatttaaat attcatcgac aataatcgat agtctcgaat gtatcggaac gtgcctgatc
     8461 cctgctttag tcttactttt gctgatataa aaacagcgta ttccttccac agttacaacc
     8521 tctgactttt tcagacttgc cagttcgtct aatctgcatc ctgaaaacaa gcccattaaa
     8581 ataatatttc tgatctccgg ctccgctgca tcaagtagct tttgcatttg ttcaggtgta
     8641 aacggttgat aactctcgat tgtgcgacgt gcttcgagat tatgaccgtg aaaagggtta
     8701 ctgtctggta ttccgtcata acggcgttta gcaaattcat agagcgatcc taagcatgta
     8761 agccagtttt gacgagattg atcggctgct cctcgttgct tctggtcatc taagaatact
     8821 gttacaagcc gtctgcctat tttctcaata tgaatatcat ctttctgaat gctttgtaaa
     8881 aatagctcaa cggcccttgc tgatttctgt agtgttgagt atgaacgacg ctctctgtag
     8941 cattcggcgt attcgtcacg tattaagcat agggaaggcg atgcataacc gcgttttacg
     9001 gctctttgcg tgtttttatt ggcccttgcc tctgaatgta aggattcaat agcttgttgt
     9061 aacctccttt cggggttatc aggattatgt ttccgcttta gttcctccca ttctaataat
     9121 agatggtttc tgaatcttct cgcctcattc agatctcgtg tagatgtgct ttttctccac
     9181 aacttgtaag ggtaaagatg acgtaagtat ttaggaacaa aaatctgaaa catccagatc
     9241 ccatcttttc tcttaaataa gtatgcttgg ctcttgatcg acacgataat gatcctctat
     9301 tatcacgtaa tcagttacct atgattgatc acaaaatctt tattttacag caggttatta
     9361 tgggtttcct cgattccctt cgcccgctcc atgaccattc ttaaagtcta ctaacttact
     9421 gaatcctaag ggatacggct taaagccttc cgatagtttt actcactgta accaacactg
     9481 tgactaactt tgttgaagta gtgagcagac ctaaggtctc agtacatgct caattcaaga
     9541 acctaccttt atcagcgtaa cggtgtcttt tacatccgtc tcaggatgaa gaccactggt
     9601 cgcctgaccg cttcgcttcc ttctcacaac cggtataaac tagcgtcagt atcactacgg
     9661 actaaggaca gacgtaccgc tatggctcac tcacggcaca tcaagtcagc acttaaagca
     9721 atacacgcag acaaccctag cgcatcatat gaggaactac gggagcacct gaagaccatt
     9781 gttgagtggg aacttggtgt cagccgtgat gacctgaacg acccggagtc ctaccagctt
     9841 tacgttgacc agtacgacga catcaagtcc aaccttcggg aagctgtagc gactgagcgt
     9901 ctgactgtag accagcatcg ctatatcaat gacgttatcg gtgtacttaa agcctgtcag
     9961 gatagactca agggcgataa ctctggcctg ttgtcttacc ttgagcctga gactggcagt
    10021 ctaagaccgt ccgtctccct atctgtatca tcggagcctg aagtccctga acctaaagca
    10081 ctgaccctcg cgtctctcat tgagcagtac gagcaggaga acgcccagaa ctggaagcca
    10141 gcgaccctaa gcgaaaaccg agcgtcacac tctacgctaa tcgagatatt cgactatctg
    10201 gatattcagg atgtcggcaa ggcgacccgt gcagatatgc ttagggtccg tgaagtcctc
    10261 caacagctac cgaagaaccg caagcaacgc tttaagtcta tgccgctgtc tgacctgctg
    10321 aaccgggagt ctaagactga ctgtcttgat gtcgttacca tcaacaataa atatcttatc
    10381 aagatggctg ctgtctttaa gtgggcggta cgtaacgacc tgatagctaa gaacctgacc
    10441 gaaggtcttg agctgaaagt cccgcagcgt aaagcctccg acgctcgtga tgctttcagt
    10501 cctgagcagg tagggcaact actggtcgcc gctaaagcgt actctcagaa gaccgcaggt
    10561 aagccatacc attattacgt caccgctctg gctgcgatta caggtgcgag acttaacgag
    10621 gtggcccagc ttcaggtcaa agacgtcagg gctactgagg ccgggaccgt atatatccat
    10681 atcaacgaag acgatagcag cctaccgggt aaaagtatta agaacgctca tagcgaccgc
    10741 tgtgtcccgc tggtcgatgg cgcttatggt tttgtattgg ctgactttat gtcgcttgtg
    10801 gaagaccgta gaaagactgc gggtgataat gctatggtat tcgatggcct caagctgatg
    10861 aagaatggct acggtgagca ggtgagcaaa tggtttaaca gaacgttgct gcctaaagtc
    10921 cttgctgacc gtagcggctt agcctttcac tcattcaggc acactgttgc gacccagtta
    10981 aaacaacatg gggtcgagtt agcgtatgct caggcgatta tgggtcatag ctcagggtct
    11041 ataacttacg accgttacgc taaagaggtc gaggtggata gattggttaa tgtgatggca
    11101 ggtgtttata aggagactgg attaaatggc tgagtattat ccggcggtgt ttgaagcaga
    11161 ggcttttaac tgtcctcact gtggtgtata tgcccgtcag ttctggcgta ggatgtatgg
    11221 aagtgcgcgt gagttggcgc aaaggacaac cgagttccga atgtcaacat gctctcattg
    11281 cggtgatgat gcttattggt atgacggcaa tatgattatt ccggcggctg gcaatgttga
    11341 actaccaaat ccagatatgc cagataactg caagtccgac tatatggaag cccgaagcat
    11401 cattaatctg tctcctaaag gggccgctgc actccttcgt ctttgccttc agaagctaat
    11461 ggtccactta ggcgagccgg gagaaaacat taataaagac atccggtcac tcgtccagaa
    11521 aggactacct gtacgcatcc agcaggcggc agatatctgt cgtattgtcg gcaatcaggc
    11581 ggtccatccc ggagagatta gtcttgatga tgacccgcag ttggcacacg gtctctttaa
    11641 gctgctgaac attattgtcg atgaccgaat cacgagaccg aaagaaattg aggctatgtt
    11701 ccagagtatg cctgaaggtc cacgtcaagg tattgagaat caggacagac aggcgaggga
    11761 gcagcagcag gccgctaacg aatgaaaccc acactatagg ggaagggggg ggactaaact
    11821 atcactatag gggagagacc ctaagtcccc ccgggcttaa tacagccacc gacccacaca
    11881 ttcattaagt ctcaggcttc cggtagcgga ccgattggaa accatccgca ataagcctag
    11941 tgttaaatca ccttggcctg agaccattcc ctttaacgta gcaatctctc tcctttgaca
    12001 ggcggagaac atcagaccgc agcggaagta tccgtctatg cgaccatcgg tgtgcgagat
    12061 tgtgaagaac caagacatca ttgctgaggt ctgccatcca tttgtggaca ggcggaaact
    12121 caaaaaggtt agaccagtgg tgtctgaggt tggagctttg agcctgacca gcaggcttga
    12181 ggagaccatg agttcatctt gttatggatt gtgttatcac aattccaata agaagaactt
    12241 taagtccagc ctccagtcct gtcagcccga agtatactac ggtcgctatg atgtggttct
    12301 ccatacttcg ggtctaactg gaagtactga acactttcgc gtcaggttct ccgaagttca
    12361 ttcctgtttg actaaactat cactataggg accatatctc gcctctgacc tcctgagaga
    12421 cggcaagcga gaaaccgtgg tccacgctag cttgctagcc accagcctct ggtcagcctt
    12481 tgtgttcttt ggtctcgtga cttaagtcta tcaccacaca cgctcggaca tgctgtcgaa
    12541 ccttcatctc tatggtcatc gcagaccact aaccccaatt acctcaagtc attctgtttg
    12601 cgtcctgttg cctgctggtc tccctttacg tgggggcttg agtaggtcag tgggggtgcg
    12661 acttcagggt gcaaacaaaa ggagtacacg taaatgtctc aacaagaaat cagcgtcatt
    12721 aacctcgacc agcttgtctc catgacctca gttgaaattg ctgagctgac aggcaaggag
    12781 caccgaaatg tcctacgtga tattcgcaat atggctgagg agcttaacgc gctcaaaact
    12841 gagcagtgct caaaactgag cagtcatatc ggggtgactg aagatgtcta tctaaacgcg
    12901 caggggaaac agcagcctct gtaccgtctc gaccgtaagc atactttcat tctggtcgct
    12961 ggctattccg ttcacctccg cgccaagtgt tacgaccata ttcagacgtt agagcgtcga
    13021 gtccttcaac tggaagacca gaagaagcga gcagccattc agtccgctaa ccgccgaggc
    13081 gtgacttggg gcgactactg caaaacctat ggtctacccg ctcagaagct aatgaccgca
    13141 cttcttcagc atcgtggtct ctttcgtaag aacccaatca gtaacgagtg gtctgtaaat
    13201 ccaaagtatt ccgactgctt ccgtatcatt aagccgtcag accagaagtt ctcagcaggt
    13261 ggttataact ttcgcttcaa cgctaaggga ttggaggtct tcggtaagcc tgcgatagtc
    13321 gataaactgc gaggcatcct tattgccttc actggcaccg accagcagaa gcaagagcat
    13381 ctcctgaagc aagcccaatc cggcaagctg gagggactct aagatgattg acccgcgcac
    13441 acctattggt cgtgcaactc tccgctaccg tggtctgcct acccgtcacc tcctgtccct
    13501 gctgagactt ggagtcgata accctgatcg cccttactac agtcgagacg agctgatagc
    13561 gatgctggtc gaccgtgatt tgaataatca gttacgtcga gcctttgcga aactggagtc
    13621 ttaacatctc cggaaatttc cagaccttaa ccaaccttat ttaaaacctt ttgagatgac
    13681 cgatgcgggg agacccgtag gcattactgt gtctgcgata ccgatagtct caactctcaa
    13741 gaggagtaat tactatgtct a
//
LOCUS       DAZFKM010000134        13723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFKM010000134 DAZFKM010000000
VERSION     DAZFKM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..911
                     /gene="hlyE"
                     /locus_tag="ACHKAY_004077"
     CDS             <1..911
                     /gene="hlyE"
                     /locus_tag="ACHKAY_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO5702656.1"
                     /translation="TEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIK
                     ELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDE
                     YNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSE
                     KSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQ
                     NFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEA
                     AKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1118..1564)
                     /gene="ycgN"
                     /locus_tag="ACHKAY_004078"
     CDS             complement(1118..1564)
                     /gene="ycgN"
                     /locus_tag="ACHKAY_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO5702657.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1656..2315)
                     /gene="ycgM"
                     /locus_tag="ACHKAY_004079"
     CDS             complement(1656..2315)
                     /gene="ycgM"
                     /locus_tag="ACHKAY_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO5702658.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2387..2680)
                     /locus_tag="ACHKAY_004080"
     CDS             complement(2387..2680)
                     /locus_tag="ACHKAY_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO5702659.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2692..2850)
                     /locus_tag="ACHKAY_004081"
     CDS             complement(2692..2850)
                     /locus_tag="ACHKAY_004081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702660.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            2921..3322
                     /gene="pliG"
                     /locus_tag="ACHKAY_004082"
     CDS             2921..3322
                     /gene="pliG"
                     /locus_tag="ACHKAY_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO5702661.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3425..3793)
                     /gene="ycgJ"
                     /locus_tag="ACHKAY_004083"
     CDS             complement(3425..3793)
                     /gene="ycgJ"
                     /locus_tag="ACHKAY_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO5702662.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4313..5008
                     /gene="minC"
                     /locus_tag="ACHKAY_004084"
     CDS             4313..5008
                     /gene="minC"
                     /locus_tag="ACHKAY_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO5702663.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5032..5844
                     /gene="minD"
                     /locus_tag="ACHKAY_004085"
     CDS             5032..5844
                     /gene="minD"
                     /locus_tag="ACHKAY_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO5702664.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5848..6114
                     /gene="minE"
                     /locus_tag="ACHKAY_004086"
     CDS             5848..6114
                     /gene="minE"
                     /locus_tag="ACHKAY_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO5702665.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6486..6830)
                     /locus_tag="ACHKAY_004087"
                     /pseudo
     CDS             complement(6486..6830)
                     /locus_tag="ACHKAY_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6865..6984)
                     /locus_tag="ACHKAY_004088"
     CDS             complement(6865..6984)
                     /locus_tag="ACHKAY_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO5702666.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6945..7130)
                     /gene="ymgJ"
                     /locus_tag="ACHKAY_004089"
     CDS             complement(6945..7130)
                     /gene="ymgJ"
                     /locus_tag="ACHKAY_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO5702667.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7231..7404
                     /gene="ymgI"
                     /locus_tag="ACHKAY_004090"
     CDS             7231..7404
                     /gene="ymgI"
                     /locus_tag="ACHKAY_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO5702668.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7406..7750
                     /gene="ymgG"
                     /locus_tag="ACHKAY_004091"
     CDS             7406..7750
                     /gene="ymgG"
                     /locus_tag="ACHKAY_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO5702669.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7754..8089
                     /gene="ymgD"
                     /locus_tag="ACHKAY_004092"
     CDS             7754..8089
                     /gene="ymgD"
                     /locus_tag="ACHKAY_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO5702670.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8146..10467)
                     /locus_tag="ACHKAY_004093"
     CDS             complement(8146..10467)
                     /locus_tag="ACHKAY_004093"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO5702671.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10584..10793)
                     /locus_tag="ACHKAY_004094"
     CDS             complement(10584..10793)
                     /locus_tag="ACHKAY_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702672.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11193..11411)
                     /gene="ymgF"
                     /locus_tag="ACHKAY_004095"
     CDS             complement(11193..11411)
                     /gene="ymgF"
                     /locus_tag="ACHKAY_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO5702673.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(11543..13066)
                     /gene="pdeG"
                     /locus_tag="ACHKAY_004096"
     CDS             complement(11543..13066)
                     /gene="pdeG"
                     /locus_tag="ACHKAY_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5702674.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13398..13646)
                     /gene="ymgC"
                     /locus_tag="ACHKAY_004097"
     CDS             complement(13398..13646)
                     /gene="ymgC"
                     /locus_tag="ACHKAY_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO5702675.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3928 a         3194 c         2950 g         3651 t
ORIGIN      
        1 tgactgaaat cgttgcagat aaaacggtag aagtagttaa aaacgcaatc gaaaccgcag
       61 atggagcatt agatctttat aataaatatc tcgatcaggt catcccctgg cagacctttg
      121 atgaaaccat aaaagagtta agtcgcttta aacaggagta ttcacaggca gcctccgttt
      181 tagtcggcga tattaaaacc ttacttatgg atagccagga taagtatttt gaagcaaccc
      241 aaacagtgta tgaatggtgt ggtgttgcga cgcaattgct cgcagcgtat attttgctat
      301 ttgatgagta caatgagaag aaagcatccg cccagaaaga cattctcatt aaggtactgg
      361 atgacggcat cacgaagctg aatgaagcgc aaaaatccct gctggtaagc tcacaaagtt
      421 tcaacaacgc ttccgggaaa ctgctggcgt tagatagcca gttaaccaat gatttttcag
      481 aaaaaagcag ctatttccag tcacaggtag ataaaatcag gaaggaagcg tatgccggtg
      541 ccgcagccgg tgtcgtcgcc ggtccatttg gattaatcat ttcctattct attgctgcgg
      601 gcgtagttga agggaaactg attccagaat tgaagaacaa gttaaaatct gtgcagaatt
      661 tctttaccac cctgtctaac acggttaaac aagcgaataa agatatcgat gccgccaaat
      721 tgaaattaac caccgaaata gccgccatcg gggagataaa aacggaaact gaaacaacca
      781 gattctatgt tgattatgat gatttaatgc tttctttgct aaaagaagcg gcaaaaaaaa
      841 tgattaacac ctgtaatgag tatcagaaaa gacacggtaa aaagacactc tttgaggtac
      901 ctgaagtctg ataagcgatt attctctcca tgtactcaag gtataaggtt tatcacattt
      961 aaaaacacag acgtttttta aagattgaga gttacctgtt gataataagg tatcgggtaa
     1021 ctctcattat gacagagcgc tttcaaaaac agggctacga aatggcgagc atccagacgt
     1081 atattaagaa agcccccatc aacatctatt aaatttatca ctgcgcccag tcaggtttat
     1141 ttaagatatg atcctgccag tcaatcactt ctgattcttt cactgcgata tgacgcacag
     1201 agatacgttc accatgcatt gccgctttcg aacccgtaag tagcggatgc cacgcaggta
     1261 aatctttacc ttccgccagc aaccgataag cgcaggtcat tggcagccat tcgaatgttg
     1321 gcagattttc acgggttaat ttaatgcagt cgggttcaaa ctcgaaacga cgttcgtagt
     1381 tccgacattg acaggtttta atattgagct ggcgacaggc gacgttagtg aagtagattt
     1441 cgtcggtgtc ttcatccatc agtttatgca ggcaacactg accgcaacca tcacacaacg
     1501 actcccattc cgcatcgctc atttcgtcca gggttttact ttgccagaaa ggtacatcgc
     1561 tcatcaggtg ttccgccatt acgttaaaac gcaccttata accagtctgg cacagcgatg
     1621 caagttttgc cgccgctttc aggcggcaaa aagtattaca aaacgcgagt tgccaaagaa
     1681 tgcccatcga aagtgactgt cagctcatca ccgctttgca acgggccgac gccatcaggc
     1741 gtgcctgtca gcacaacgtc accggccttg agggtaaaaa acttgctcat ataagcgatc
     1801 agcggaacaa ttttatggat catgtccgca gtcgtacctt gctgacgttg ctcgccgttt
     1861 acgctcaggc ccagcgttgt attttgcggg tcgccggtaa attccgccgc gggaataaac
     1921 ccggaaagcg gacaagagtt atcaaacgct ttagcctttt cccacggctg cccggctttc
     1981 ttcatttttc cctgaacatc acgcaatgtc agatcgagcg ccacgccata accggcaatg
     2041 gctttgcgga catgctcttc cgtagcctga cgcagcgtcg cgccaatcaa caccgccagt
     2101 tcgacttcat gatgaactga accgaaatcg gatgggatcg ccaatggctg ccgcagatcg
     2161 cacagtgccg tttctggttt aataaacagc actggctctt cgggcactgc gctgcccatc
     2221 tctttaatat gtttggcata gttactgcca acacagacta ctttactcac cggataatcc
     2281 agcagcgcac cttgccagtt gtgatgttga tacataacgt tcccctcacc ttatcgtgga
     2341 tatgaaaacc cgcgccgaca aagataatgc caccgccgga tatcggttat ttgttagtgt
     2401 cgtctgtttt ctgccccatg acggaaagat gttgcttcag caaatcttcg ggtggtggcg
     2461 gtaactgcaa atagtaacct tgctcggtta atgccagttt aaccttttca atatcagcat
     2521 tgaccagttt cttccgccca tccaacggca gaatcatcgc taactgaggc tgaccaaaac
     2581 ctttcatcag ttcctcagga acacgcgaaa aatcgtcttt tttttcgaca tataaatagg
     2641 tctggtcacg cttgctgctt cgataaatca cacaaaacat acttttactc tttaagatgc
     2701 caggttttgg cataagctaa catcaatgaa acccgcgcca caagccagcc agctgtagat
     2761 ttctacggtt tgttgagtcc atgccccttt ataagtaatc cccccggcaa aaatgccgcg
     2821 ccgggatttt accgaacgca ggaaagccac tggaattctc ccgttaggga ttgttatatt
     2881 ataacagttc atcgtactca ttctgaacag gagactacca atgaaaatca agagcatcag
     2941 taaggctgta ttgctgtttg cactgctaac ctccacgagc tttgctgcgg gtaagaatgt
     3001 caatgttgag ttcagaaaag gccatagcag cgctcagtac tccggcgaaa taaagggata
     3061 cgattacgat acatatacct tctatgccaa aaaaggtcag aaagtgcatg tgagtatctc
     3121 taatgaaggc gctgatactt acctcttcgg gccaggaatc gatgattcag ttgatttatc
     3181 cagatattcc ccagaactcg atagtcacgg acaatactca ctccctgctt ctggcaaata
     3241 tgagctgcga gtgttgcaaa cccgaaacga cgcccgtaaa aacaaaacta aaaaatataa
     3301 cgtcgatatt caaattaagt aaatgtcaac cgacggggac tctccccgtc ataaacgtta
     3361 acgccccact cctctctgtc gctcaagagg ttaatgacga atagaacata atgtaaacaa
     3421 gacatcactc acgacacata aataacatct ttgtggtggt ttgattgatt tttccactcc
     3481 gcttaccatc tacaccaaaa taacgatcat cacgacataa tttttcttta acatcgcaaa
     3541 atacaccgtt agcgaatgta aattcgctgg gatcgtagcc ttgtaatgat tgtaagtttt
     3601 cagcggcttt ttcgccgaga tatttccttg ttaactctgg ggaaatgcca tctgagttca
     3661 cacaaatata cgggtcgcaa acaacgccgc acgccggaga ttgaagtccg ctagcgtcat
     3721 gtccagacgc cattgatgag aacatcatcc ccacacctga caatccgata taaataatac
     3781 gcatcatatt catagcacat cctgataatc caaccgtaca acacttatgc tcatttcgtc
     3841 atcaatccgt aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca
     3901 gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag ataacgggta
     3961 tctgttcttt aatgatgccg caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa
     4021 atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca cgattagggt
     4081 gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg gttgattgac
     4141 aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact tttactctga
     4201 ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct tatagtcttc
     4261 ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca ggatgtcaaa
     4321 cacgccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc tgcatgaggc
     4381 agaacctaag gttatccatc aggcgctgga agacaaaatc gctcaggccc ccgcattttt
     4441 aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa actggtcagc
     4501 gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga
     4561 tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg aaggaaagga
     4621 aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa cgccggtcac
     4681 aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg ctccacaatg
     4741 tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg atggtaacat
     4801 tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac
     4861 gcaaatattt tgtacgaacc tgatggcgga actggtgtcc atcgcaggtg aatactggct
     4921 gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt tagtcgaaaa
     4981 cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc tatggcacgc
     5041 attattgttg ttacttcggg caaagggggt gttggtaaga caacctccag cgcggccatc
     5101 gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga tatcggcctg
     5161 cgtaatctcg acctgattat gggttgtgaa cgccgggtcg tttacgattt cgtcaacgtc
     5221 attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc
     5281 tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga aggggtcgcc
     5341 aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga ctccccggca
     5401 gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat tattaccacc
     5461 aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct ggcgtcgaaa
     5521 tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt aacgcgctat
     5581 aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct ggagatcctg
     5641 cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc ctctaaccag
     5701 ggtgaaccgg tcattctcga cattaacgcc gatgcgggta aagcctacgc agataccgta
     5761 gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa gaaaggcttc
     5821 ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct ctcgcggaag
     5881 aaaaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc
     5941 agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga ggtcatttgt
     6001 aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa agatggcgat
     6061 atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa ataagcccgc
     6121 tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt tcatccctct
     6181 catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg ctatttccac
     6241 ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta tgcctggttg
     6301 agtaaataac ctcattgtta gtgtggatac actgggggat gataaccccg caatttcatt
     6361 attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt tttttgcccc
     6421 agaattttcc cttttagccc cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt
     6481 gatggtcaga acgaccagtt cacaccagcc accgcattcc acggggattc cacaccggca
     6541 ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga ggctttaata
     6601 cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc gtcactattc
     6661 actttcaccc gattatcatc gacaaattct ttgctcacag ccgccttcag gcagggttca
     6721 atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat
     6781 atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata gtagcgtcta
     6841 agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg
     6901 aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg
     6961 atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt agttggataa
     7021 aaaatcactc ttttcagata atactgtcgt acgcttatct ttcgacaatg aggccgtcgg
     7081 caaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca
     7141 cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg
     7201 acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc
     7261 attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat
     7321 aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca
     7381 acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc
     7441 agcattgttc tgctccacgc cagcgatggc ggatatgaat cgcaccacga aaggtgcatt
     7501 gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc
     7561 agctgttggc gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg
     7621 taaaggtgca aaagtaggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg
     7681 cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa
     7741 aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc
     7801 accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat
     7861 tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt
     7921 tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga
     7981 actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct
     8041 gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg
     8101 taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga acttatattt
     8161 caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt
     8221 cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa
     8281 attactcgcc ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact
     8341 gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt
     8401 tttctcctga gtacgaataa aggtgcggaa accaagtcgg gtctggatgt tgttatttgc
     8461 gtcattctca atacgtgagc cattggcttc ggtgtgcgca ttatgatcga cgcccatcca
     8521 gatagcctgt gcctgtggct ggaatatcca cgtgtatttc gccgcttcca gaccaaagga
     8581 tttattcagg gcaaagccgt aacccgcttc cagcgagacg gttgcgcctt tcgcactata
     8641 ggattcatag gctaattcat cccctttcac cgagttttta aaccagctat attgcgccca
     8701 ggcgtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg catacagccc
     8761 tgcgcaatag cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttga
     8821 gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac tgtcggtaaa
     8881 accaccggtt aacagctcgg cccctaactg cgagacgtag cgatgcgagg tcgttctcaa
     8941 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct
     9001 tgaacgctcc tgttcagtta caggatcgat gtagcgcatt tcacccgcac ggtcgttcag
     9061 acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc cagcttcgag
     9121 gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt ttgtttcacc
     9181 gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt cggtattacc
     9241 tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa taaattcccc
     9301 atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag ctccgctgcc
     9361 tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa taacgacttt
     9421 atccgtagct gaattatcat cacccaattc agttctgaga tgcaatacgc cattattacc
     9481 aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat ctgcccggga
     9541 aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag ctttaatatc
     9601 ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttaacatctg ttgaaatatt
     9661 agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt taatattatt
     9721 gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt tactcgtctc
     9781 atttttatgg tagaggattt catttgctga ccctagtgag ctaccagata aaatatctac
     9841 cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct gatgcgcgac
     9901 tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcag tcgcggcgtc
     9961 aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc gggcagcgat
    10021 ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt
    10081 ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg cggcataact
    10141 ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta tggcattatt
    10201 ttcattactt cctgtatcgg tagtactttc ttcatcaaga gaagatacac acgcacgcgc
    10261 gcttccggga gatgcacacg caagaacgcc gactgcatga ttacccgcag taacaataaa
    10321 tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga ctcttattat
    10381 ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt aatcccccgt
    10441 tatgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac ttacgattgc
    10501 gctagctgtt aatgctgtgc ttagaggact attgtcagaa ggatataaac cgcccgttgt
    10561 tacaatgacg ccattgttaa tggttaactt gctaccctct cctgcaacgg atacccgtaa
    10621 cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt tccatctttt
    10681 attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata tgcattaccc
    10741 accgccagag caataagtcc caaactaaac ccggagagtt ttttcagttt catagattaa
    10801 ccatccattc attggattaa caaacattcc tggtcaaata acctatgcgt tcagattacc
    10861 aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata aacagcgaca
    10921 atatataaat gataaataag tcggcattat catttttcac cttattttca tgacattgat
    10981 cactttgagg tgacgatttt gtttaacaaa taactcttcg acatcacaat cattgatagt
    11041 tgcaataaaa attatttcaa tgacatataa aaacaactgt gataacacca gggttgacaa
    11101 ctatttgcgc aataattcct ttacttcaat gatccaggca ttcactgcca gcaacccatt
    11161 cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg gctgacgagt
    11221 ctttccctta atcgattaac cagattgatc gccagccaaa caagggcatt aagcaaattt
    11281 atcgtcacaa gcacttctcc cagcccggta ctgggttttg caataaggat gaccaggagg
    11341 gtgatcagca taaatgcaat ggaaaagatg atatagagag tgaaataatc cagattatta
    11401 ctattgttca tgctactaat ggcaatcctg tcaaaacttt caaacaatta tagcaaacaa
    11461 aataatgaac aatagtaaca agaggcaacg agctaattag ctgtctataa ttacaccgca
    11521 ctatggcctt actgatcaat tttcactcaa ccacaacctt caccttcggt ttagaaagga
    11581 taattttgac caggtcgatg tatgtaacag gtttatagaa ataataaccc tgctgaaatg
    11641 tgatattatt ttggttcaga tagtcaagtt gttcattcgt ttcgacacct tcagcgacga
    11701 tactgatcga aagtttacgc gccagatcca atacacaatc cagaattcgg gttgattctg
    11761 ggtctgcgtt tacgcggcca acgaaactgt gatcaatttt gatataatca atatgcaggt
    11821 catgaagata agaaagccct gagtagccag taccgaaatc atccagcgcg atgacaaaac
    11881 cattttcatg cagaatgttc aaccgctgaa ccagactttc atcaacattc aatggctcac
    11941 gctcggtgac ttccagaaca aggtttaaat cgcggcgggt aaaactgtca cggaaattta
    12001 aacactcgtc gacaaacgtc ggcgaaataa tatgcgaagc gctaaaatta attccaatat
    12061 gaaaaccttc cggcaattta ctcgcgatag cgttcatctg tctggcaacc tgattcatca
    12121 ggctttgcgt aagcggaacg attaatccgg atttttcagc aagtggaata aatgccgcgg
    12181 gtgatatata tccaccgtga ggttgtttcc agcgggctaa cacctcaact ccccgcaatg
    12241 tcccttcccg accatttacc acaggttggt aaaaagggac gatctcccca ttatttattg
    12301 cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa atatctatca agcaggaagg
    12361 cagcggcgca ggcgattaac agaataaata ttaaaatccc aaagccttta tcgataagtc
    12421 tgtagaagct aaagagtggc ggcgggttaa agataatagt gaaatgatat tttgtggagt
    12481 gaaccttctc ggtataggga taatgtccat taagcgtcat cacatcaccc gttggtccaa
    12541 taatgtcatc cgccacgcgt aatacatacc ttaccccttt caagggtaca ttaagtgccc
    12601 cacgaatatg ctgatcgctt atcgtaacca aaatgcgcgt atctgcaaat tggttctgat
    12661 agagcaggat aggtaatcta ttaacggtgt cgattgctgg agccaacagt aaattactgt
    12721 ccgggaaaac aggaatacga gacaacagga cccgattccc aggcagggat gtacaccaga
    12781 ctattccctg tttgagaatg atgattgtgc gcaggtgagg tttcagagct gcttcagtgc
    12841 caagctgata ttgctcctcg aggttgcatt ccttcccggc aatgttaacc gctgtttgag
    12901 tcgcttgtga cgcttcttca agtatatgat tgatattgtt ggcggcatat ctcgctcccg
    12961 ccaggtactc tgcttttgcg gaataccaga gctggatgtt caaaatagcg acaccggtaa
    13021 taaacaagca tatcgcaacg aggatgggta tgagtgtatt gcgcatttgc ttgtcccggc
    13081 caaagggttt aatacatgaa aatgccattc attcgctcgc gctggcatct acagagagca
    13141 tggtagagag cataagaaat atccacgccg aagataaaat tttctccatc ttgatctaac
    13201 ccattaatta ttgagcataa tgtaggcagt acaaaataag ttaggcggga tatcaggcgt
    13261 caagaatgga gagcgagaac tctccattct tgacacctga tattgcggac ataataagaa
    13321 agcataacgc ctgaaatgct cactttgcat cagcatggtg atacagctga tgtttattct
    13381 aaaaccttac tcaagttcta agagagcacg gattccctgt cattaataat gatatccgtt
    13441 agtgtagtct tatgcatttc aatgtactgc aatatttgca gatagagatc agaaagaatc
    13501 acactggttt catcttttaa acgcaagcgt aaagcctgat caataatatg actatcaaca
    13561 tacccatgcg tcatgaacac ctccctctcg agatttttaa acaaagcaca ttgaaaaata
    13621 aatctctctg ggattgaatt attcatgtca gcctcgtaga aatattaacc caataaaatt
    13681 atgctgtgta tagtctaaag cgacagacaa aaataagatg aat
//
LOCUS       DAZFKM010000135        13647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFKM010000135 DAZFKM010000000
VERSION     DAZFKM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            267..1376
                     /gene="livK"
                     /locus_tag="ACHKAY_004098"
     CDS             267..1376
                     /gene="livK"
                     /locus_tag="ACHKAY_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO5702676.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1424..2350
                     /gene="livH"
                     /locus_tag="ACHKAY_004099"
     CDS             1424..2350
                     /gene="livH"
                     /locus_tag="ACHKAY_004099"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO5702677.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2347..3624
                     /gene="livM"
                     /locus_tag="ACHKAY_004100"
     CDS             2347..3624
                     /gene="livM"
                     /locus_tag="ACHKAY_004100"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO5702678.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3621..4388
                     /gene="livG"
                     /locus_tag="ACHKAY_004101"
     CDS             3621..4388
                     /gene="livG"
                     /locus_tag="ACHKAY_004101"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO5702679.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4390..5103
                     /gene="livF"
                     /locus_tag="ACHKAY_004102"
     CDS             4390..5103
                     /gene="livF"
                     /locus_tag="ACHKAY_004102"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO5702680.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5499..6815
                     /gene="ugpB"
                     /locus_tag="ACHKAY_004103"
     CDS             5499..6815
                     /gene="ugpB"
                     /locus_tag="ACHKAY_004103"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO5702681.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6913..7800
                     /gene="ugpA"
                     /locus_tag="ACHKAY_004104"
     CDS             6913..7800
                     /gene="ugpA"
                     /locus_tag="ACHKAY_004104"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO5702682.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7797..8642
                     /gene="ugpE"
                     /locus_tag="ACHKAY_004105"
     CDS             7797..8642
                     /gene="ugpE"
                     /locus_tag="ACHKAY_004105"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO5702683.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8644..9714
                     /gene="ugpC"
                     /locus_tag="ACHKAY_004106"
     CDS             8644..9714
                     /gene="ugpC"
                     /locus_tag="ACHKAY_004106"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO5702684.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9711..10454
                     /gene="ugpQ"
                     /locus_tag="ACHKAY_004107"
     CDS             9711..10454
                     /gene="ugpQ"
                     /locus_tag="ACHKAY_004107"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO5702685.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10441..10881)
                     /gene="yhhA"
                     /locus_tag="ACHKAY_004108"
     CDS             complement(10441..10881)
                     /gene="yhhA"
                     /locus_tag="ACHKAY_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO5702686.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11001..12743
                     /gene="ggt"
                     /locus_tag="ACHKAY_004109"
     CDS             11001..12743
                     /gene="ggt"
                     /locus_tag="ACHKAY_004109"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO5702687.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12781..13065)
                     /gene="yrhB"
                     /locus_tag="ACHKAY_004110"
     CDS             complement(12781..13065)
                     /gene="yrhB"
                     /locus_tag="ACHKAY_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO5702688.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(13265..13420)
                     /gene="yrhD"
                     /locus_tag="ACHKAY_004111"
     CDS             complement(13265..13420)
                     /gene="yrhD"
                     /locus_tag="ACHKAY_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO5702689.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            complement(13515..>13647)
                     /gene="yrhA"
                     /locus_tag="ACHKAY_004112"
     CDS             complement(13515..>13647)
                     /gene="yrhA"
                     /locus_tag="ACHKAY_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFO5702690.1"
                     /translation="ISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT         3083 a         3421 c         3838 g         3305 t
ORIGIN      
        1 cctgaatggt agtgaattat tccgcccttt gtgccgttat tttatgctga caaaggcact
       61 tttttctgtt tatctatcaa taaattcaga atattatctg ttcttaatcg actgaaaaat
      121 agggatttta atcactatta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa
      181 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
      241 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
      301 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
      361 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
      421 cgattaaaga cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat
      481 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg
      541 gcattaaata cgttattggt catctgtgtt cttcttctac ccagcctgcg tcagatatct
      601 atgaagacga aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac
      661 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg
      721 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
      781 aatatggcga agggctggca cgttcggtgc aggacgggct gaaagctgct aacgccaatg
      841 ttgttttctt cgacggcatt accgctggcg agaaagattt ctccgcgctg atcgcccgcc
      901 tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
      961 tgctgcgcca ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtgg
     1021 gtaatgcgtc gctgtcgaat attgctggcg atgctgccga aggcatgttg gtcaccatgc
     1081 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
     1141 agaaagatcc gtccgggcct tatgtctgga tcacttacgc ggcggtgcaa tctctggcaa
     1201 cagcccttga gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
     1261 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
     1321 ttgattttgg tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgatcat
     1381 cccaccgccc gtaaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
     1441 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
     1501 catcggctac accatggttt acggcattat cggcatgata aacttcgccc acggcgaggt
     1561 ttatatgatt ggcagctacg tctcatttat gatcatcgcc gcgctgatga tgatgggcat
     1621 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc
     1681 ctacggctgg agtatcgaac gggtggccta tcgcccggtg cgtaactcta agcgcctgat
     1741 tgcgctcatc tcggcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga
     1801 aggttcgcgt gacgtggcgc tgccgagcct gtttaacggt cagtgggtgg tgggccatag
     1861 cgaaaacttc tctgcctcta ttactaccat gcaggcggtg atctggattg tcaccttcct
     1921 cgccatgctg gcgctgacga ttttcattcg ctattcccgt atggggcgcg cgtgtcgtgc
     1981 ctgcgcggaa gatctgaaaa tggcgagtct gcttggcatt aacaccgacc gggtgattgc
     2041 gctgaccttt gtgattggcg cggcgatggc ggcggtggcg ggtgtgctgc tcggtcagtt
     2101 ctacggcgta attaacccct acatcggctt tatggccggg atgaaagcct ttaccgctgc
     2161 ggtgctcggt gggattggca gcattccagg ggcgatgatt ggtgggctga tcctggggat
     2221 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaggatgtgg tttcatttgc
     2281 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
     2341 gaaagtatga aaccgatgca tattgcaatg gcgctgctct ctgccgcgat gttttttgtc
     2401 ctggcgggcg tctttatggg cgtgcaactg gagctggatg gcaccaaact ggtggtcgac
     2461 acggcttcag acatccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc
     2521 cagcttttgc gaccggcttt ccagaaaggg ctaaaaagcg tttccggacc gaagtttatt
     2581 ctgcccgcga ttgatggctc cacggtgaag cagaaactat tcctcgtggc gctgctggtg
     2641 cttgcggtgg cgtggccgtt tatggtgtcg cgtgggacgg tggatatcgc caccctgacc
     2701 atgatctaca ttatcctcgg cctcgggctg aacgtggttg ttggtctttc tggtctgctg
     2761 gtgctggggt acggcggctt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac
     2821 tattacggcc tgggcttctg gacctgtctg ccgattgccg ggttaatggc agcggcggcc
     2881 ggcttcctgc tcggcttccc ggtgcttcgc ttgcgcggcg actatctggc gatcgttacc
     2941 ctcggtttcg gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc
     3001 ccgaacggaa tcagtcagat cccgaaaccg acactcttcg gactcgagtt cagccgtacc
     3061 gctcgtgaag gcggctggga cacgttcagt aatttctttg gcctgaaata cgatccctcc
     3121 gatcgtgtca tcttcctcta cctggtggcg ttgctgctgg tggtgctaag cctgtttgtc
     3181 attaaccgcc tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg tgaagacgag
     3241 atcgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccata
     3301 agtgccgcgt ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg ctttgtcagc
     3361 ccggaatcct tcacctttgc tgaatcggcg tttgtactgg cgatagtggt gctcggcggt
     3421 atgggatcgc agtttgcggt gattctggcg gcaattttgc tggtggtatc gcgcgaattg
     3481 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
     3541 atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
     3601 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggt ctgatgatgc
     3661 gcttcggcgg cttgctggcg gtgaacaacg tcaatcttga actgtacccg caggagatcg
     3721 tctcgttaat cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggat
     3781 tctacaaacc caccggcggc actattttac tgcgtgatca gcatctggaa ggtttaccgg
     3841 ggcagcaaat tgcccgcatg ggcgtggtgc gtaccttcca gcatgtgcgt ctgttccgtg
     3901 aaatgacggt gattgaaaac ctgctggtgg cgcagcatca acaactgaaa accgggctgt
     3961 tctctggcct gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg
     4021 ccgccacctg gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc
     4081 tggcctatgg tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga
     4141 ttttaatgct cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg
     4201 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
     4261 tgaagctggt gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg
     4321 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
     4381 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
     4441 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgagattgt caccctcatt
     4501 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgct
     4561 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
     4621 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
     4681 gaagagaacc tggcgatggg cggctttttt gccgaacgcg accagttcca ggagcgcata
     4741 aagtgggtgt atgaactgtt tccacgtctg catgagcgcc gtgttcagcg ggcaggcacc
     4801 atgtccggcg gtgagcaaca aatgctggcg attggtcgcg cgctgatgag caacccgcgt
     4861 ttgctgctac ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc
     4921 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
     4981 aaccaggcgc taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc
     5041 tccgataccg gcgacgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg
     5101 taataacacg ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa
     5161 aattgcctga tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg
     5221 cacgatcttg taggccggat aaggcgttta cgccgcatcc ggcatgaaac gatgggtaat
     5281 ctgtagagtt tgattcagac cttctatttt ccgcttatcc gtgccccatc tcccattttc
     5341 cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct tacaaatgta
     5401 acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag cataaaacgc
     5461 gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta cattatacag
     5521 cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg acgaccattc
     5581 cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg gcccaacgtt
     5641 ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac tacgaacaga
     5701 atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt ttgcaggttt
     5761 atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta tatgacgtgt
     5821 ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt tccggttact
     5881 actccgacag caaaacgggc cacttactct cccagccatt caacagctcg acccccgttc
     5941 tctattacaa caaagacgcc ttcaagaaag caggattaga cccggaacaa ccgccgaaaa
     6001 cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggtatg aagtgcggct
     6061 acgccagcgg ctggcagggc tggatccaac tggaaaactt tagcgcctgg aacggtctgc
     6121 cgtttgccag caaaaacaac ggctttgacg gcacggacgc ggtgctggag ttcaacaagc
     6181 cggagcaggt gaaacatatc gccatgctcg aggagatgaa caagaagggc gacttcagct
     6241 acgtcggtcg taaggatgaa tccaccgaga agttctataa cggtgattgc gcgatgacca
     6301 ccgcctcttc cggttctctt gccaacattc gcgagtacgc caaatttaat tacggcgtag
     6361 gcatgatgcc ttacgacgcc gatgcgaaag atgcgccaca aaacgccatt atcggcggag
     6421 ccagcctgtg ggtgatgcag ggtaaagata aagaaacgta taccggtgtg gcgaagttcc
     6481 tcgatttcct cgcgaagcca gaaaacgctg ccgagtggca tcagaaaacc ggttatctgc
     6541 caattaccaa agcggcgtat gacctgaccc gtgagcaggg cttttacgag aaaaacccag
     6601 gtgcggatac cgcgacgcgt cagatgctga ataagccgcc gttgccgttc accaaagggc
     6661 tgcgtctggg caacatgccg cagatccgcg tgattgtgga tgaagagctg gagagcgtgt
     6721 ggaccggtaa gaagacacca cagcaggcac tggataccgc cgttgagcgt ggaaatcagt
     6781 tgctgcgccg ctttgagaaa tcgacgaagt cttaatcagt gtaatgtcgg atgcgtttcg
     6841 cttatctgac ttggcatcgc gtgtaggccg gataagcgaa gcgcatccgg cacagttcag
     6901 gaattaaccg taatgtcatc atcccgtccg gtgttccgct cgcgctggct gccttatctg
     6961 ctggtcgcgc cgcagctcat catcaccgtt atctttttta tctggcctgc gggcgaagcg
     7021 ttgtggtact cgctacaaag cgtcgatccg tttggtttct ccagccagtt tgtcggcctg
     7081 gataacttcg tcgcgctgtt tcatgacagc tactatctcg actccttctg gacgacgata
     7141 aaattcagca cctttgtcac cgtcagcggt ttgctggtgt cgctgttctt cgcggcgctg
     7201 gtggagtaca tcgtgcgcgg cagccgtttc tatcaaacct taatgctgct gccttacgcc
     7261 gtggctcccg ccgttgccgc cgtattgtgg atcttcctgt ttaaccccgg tcgcgggctg
     7321 atcacccatt ttctcgcgga gttcggctac gactggaacc acgcgcaaaa cagcggtcag
     7381 gcaatgtttc tggtggtgtt tgcctcagta tggaagcaaa tcagctacaa cttcctgttc
     7441 ttctatgccg cgctgcaatc cattccccgt tcgttgatcg aagccgcagc catcgacggt
     7501 gcaggcccaa ttcgccgctt ctttaagatt gcgctgccgc ttatcgctcc ggtgagtttc
     7561 ttcctgctgg tggtgaacct ggtgtatgcc ttcttcgaca ccttcccggt gatcgacgcc
     7621 gccacgtccg gcgggccggt tcaggccacc acgacgctga tttataagat ctaccgtgaa
     7681 ggctttaccg gactggatct ggcttcgtct gccgcgcagt cggtggtgtt gatgttcctc
     7741 gtcatcgtgc tgacggtggt gcagttccgc tatgttgaaa gcaaggtgcg ttaccaatga
     7801 ttgagaaccg tccgtggctg acgatattca gccataccat gctgatcctc gggatcgcgg
     7861 tgatcctctt cccgctgtac gtggcgtttg tcgcggcgac gctggataaa caggccgtct
     7921 atgccgcgcc gatgacgctc atccccggca cacatctgct ggaaaatatc cacaacatct
     7981 gggttaacgg ggtaggcacg aatagcgcgc cgttctggcg gatgttgctt aacagctttg
     8041 tgatggcgtt cagcattacg ctcggcaaaa ttaccgtctc gatgctctcg gcatttgcca
     8101 ttgtctggtt tcgttttccg ctacgtaacc tcttcttctg gatgattttt atcaccctga
     8161 tgctgccggt tgaagtacgt atcttcccga cggtggaagt catcgccaac ctgaagatgc
     8221 tcgacagcta cgccggttta acgctgccgc tgatggcctc ggcgaccgcc acgtttctgt
     8281 ttcgccagtt ctttatgacg ctgccggatg aactggtgga agcggcgcgg atcgacggcg
     8341 cgtcaccaat gcgtttcttt tgcgacatcg tttttccgct ctccaaaacc aatctggcgg
     8401 cgctgtttgt gatcaccttt atttacggct ggaaccagta tttgtggccg ttgttgatta
     8461 ttaccgatgt ggatctcggc accaccgtgg cagggatcaa agggatgatc gctacaggcg
     8521 aaggcaccac tgaatggaac tcagtgatgg cggcgatgtt gttaacgctt atccctccgg
     8581 tggtgattgt tttagtgatg cagcgtgcct tcgtgcgcgg cctggtcgat agtgagaaat
     8641 aagatggcag gactgaaatt acaggcagta accaaaagct gggatggtaa aacccaggtc
     8701 attaaaccgc tgacccttga tgtggcggat ggcgaattta tcgtgatggt cgggccgtct
     8761 ggctgcggga aatcgacgct gctgcgcatg gttgccgggc tggagcgggt gacagaaggc
     8821 gatatctgga tcaacgacca gcgcgtgact gaaatggagc caaaagatcg cgggattgcg
     8881 atggtgttcc agaactacgc gctttatccg catatgagtg tcgaagaaaa catggcgtgg
     8941 gggctgaaaa ttcgcggcat gggcaagcag caaattgccg agcgcgttaa agaagcggcg
     9001 cgcattctgg agctggacgg tctgctcaaa cgtcgcccgc gcgagctttc cggcggtcag
     9061 cgccagcgtg tggcgatggg ccgcgcgatt gtgcgcgatc cggcggtgtt cctgtttgat
     9121 gagccgctct ctaacctcga tgccaagctg cgcgtgcaga tgcgtcttga actgcaacag
     9181 ttgcaccgtc gcctgaaaac gacttcactc tacgttactc acgatcaggt tgaagcgatg
     9241 acgctcgccc agcgagtaat ggtgatgaac ggcggcgttg ccgaacagat tggcacacca
     9301 gttgaagtct acgaaaagcc cgccagcctg tttgtagcga gttttatcgg cagtccggcg
     9361 atgaacctgc tgacaggccg cgtgaataac gaaggcacgc atttcgaact ggacggcggt
     9421 attgagctgc cgctaaacgg tggctaccgt cagtatgccg ggcgtaaaat gactctcggc
     9481 attcgcccgg aacatattgc gctaagctcg caggcagaag gcggcgtacc gctggtgatg
     9541 gacacgctgg agatcctcgg cgcagataac ctggcgcacg gacgctgggg cgagcagaag
     9601 ctggtggtgc gactggcgca tcaggagcgc ccgacggcag gcagcacgct gtggctgcat
     9661 ctggcggaaa atcagctgca tctttttgat ggtgaaacag gacaacgagt atgagtaact
     9721 ggccttatcc ccgcatcgtc gctcatcgtg gcggcggtaa gctggccccg gaaaacaccc
     9781 tggcggcaat cgacgtcggg gcaaaatacg gtcataagat gatcgaattt gacgcgaagt
     9841 tatcgaaaga tggcgagatc ttcctgctcc atgacgacaa tctcgaacgc accagcaacg
     9901 gctggggcgt cgcgggtgaa ctgaactggc aggatttact gcgcgtggat gcgggcagtt
     9961 ggtacagcaa agcgtttaaa ggtgagccgc tgccgttgct ttcgcaggtg gcggaacgct
    10021 gtcgcgaaca cgggatgatg gcgaatatcg aaatcaaacc caccactggc actggaccat
    10081 taacgggcaa aatggtggcg ctggcggcac gccaactgtg ggccggtatg acgccgccgc
    10141 tgctgtcatc gtttgagatt gatgctttag aagctgcaca gctggcggca ccggaactgc
    10201 cgcgtggttt attgcttgat gagtggcgcg acgactggcg cgaactgacc gcgcggctgg
    10261 gctgcgtctc tattcatctc aatcataagt tactcgataa agtgcgagtg atgcagttga
    10321 aagacgccgg actgtggatt ctggtttata ccgtcaataa accccagcgc gcggcagagt
    10381 tgctgcgctg gggcgtggat tgcatctgta ccgatgcgat tgacgtgatt ggtccgaact
    10441 ttacggccca atagttttca acggaatgtc aggctgcggc gtgctgggct gattcagcat
    10501 gtcgccgttt ctcctctccg gcagcatatg ctgctgattc aacgaactat ccggattacg
    10561 gttgctgttt aacatcccgc cgttggtgtt gggcagcatt tgctgccggg cgggatttcg
    10621 ctcccccggc tgtgactgca acacccgctg agaattattg tttatctggt tttctaaatg
    10681 ctgctgttgc aactgagttt gcgttttcag ttgctgattc agcatccctt tttgctggat
    10741 ttgctgagtc tgcatctggg tttgcatccg ctgctggctg ggtatctgat accccggctg
    10801 gttagggttg ttcagagtat taatgggctg tgcaaagccg acaaacggca ggagtgccgt
    10861 aagaatcaga agccgtttca tcgcgtatcc tcctctgaag atatccttta agtttactcg
    10921 cttcccgaca aaacgatgat taattcagag ttatatacca ggcttagctg gggttgcccc
    10981 ttaatctctg gagaataacg atgataaaac cgacgttttt acgccgggtg gccattgctg
    11041 ctctgctctc aggaagttgt tttagcgccg ccgccgcgcc tcctgcgccg cccgtctcgt
    11101 atggtgtgga ggaagatgtc ttccacccgg tacgcgcgaa acagggaatg gtagcgtctg
    11161 tggacgccac tgccactcag gtgggggtgg atattctcaa ggagggcggg aatgccgttg
    11221 atgccgccgt ggcggtgggc tacgcgctgg cggtaacgca tccgcaggca gggaatctgg
    11281 gcggtggtgg ttttatgctg atccgctcga aaaatggcaa taccaccgct atcgatttcc
    11341 gcgaaatggc acccgctaaa gcgacccgcg atatgttcct tgatgaccag ggcaacccgg
    11401 acagcaaaaa atcactcact tcgcatctgg cttccggcac accaggtacg gtcgctggtt
    11461 tctcgctggc gttggataag tacggcacta tgccgttgaa caaagtggtg cagcccgcgt
    11521 tcaaactggc acgcgatggt tttatcgtta acgatgcttt ggcagacgat ctcaaaacct
    11581 acggtagcga agtgttgccg aatcacgaaa acagcaaagc tatcttctgg aaagagggcg
    11641 aaccgctgaa aaagggcgac acgctggtgc aggcgaacct ggcaaagagc ctggagatga
    11701 ttgctgaaaa cggcccggac gaattctata aaggcacgat tgcggaacag atcgcccagg
    11761 agatgcagaa aaacggtggc ttgatcacta aagaagattt agcggcctat aaagcggtcg
    11821 aacgcactcc gataagcggc gattatcgcg ggtatcaggt ttactccatg ccaccgccat
    11881 cctccggcgg gatccatatc gtacaaatcc tcaatattct ggaaaacttc gatatgaaga
    11941 aatacggctt tggcagcgcc gatgcgatgc aaatcatggc agaagcggag aaatacgcct
    12001 acgccgaccg ctcggaatat cttggcgacc cggattttgt caaagtcccg tggcaggcac
    12061 tgaccaataa agcctatgcc aaatccattg ccgatcaaat tgatatcaac aaagcgaagc
    12121 cgtccagtga gattcgccct ggcaagcttg cgccttatga gagtaatcaa actacccatt
    12181 actctgtagt ggataaagac ggtaacgcgg tggcggtgac ctatacgctg aacaccacct
    12241 tcggtacggg tattgtcgcg ggcgagagcg gtattctgct taataaccag atggatgatt
    12301 tctccgccaa accgggcgta ccgaacgttt acgggctggt gggcggtgat gccaacgccg
    12361 tcgggccgaa caaacgcccg ctgtcgtcga tgtcgccgac cattgtggtg aaagacggta
    12421 aaacctggct ggttaccggt agcccaggcg gtagccggat catcactaca gtgctgcaaa
    12481 tggtggtgaa tagcatcgat tatggcatga acgtcgccga agcgaccaat gcgccgcgtt
    12541 tccaccatca gtggttgccg gacgagctgc gtgtcgaaaa agggtttagc ccggatacgc
    12601 tcaagctgct ggaagcaaaa ggtcagaaag tggcgctgaa agaggcgatg ggcagtacac
    12661 aaagcattat ggttgggccg gacggtgagt tgtacggcgc atccgacccg cgctcggtgg
    12721 atgatttaac ggcggggtac taaggttagc ggccctcttc gtgggaagag ggctattttg
    12781 tcagggcaag ccgaaggtag ccttttttat ttcgtaatcc tgtagatatt cttccagcgg
    12841 ctttgccgtt cccagagaat gaatttcacc actgtcttta tcaataataa aaggtgcgtt
    12901 accagctaag cgcgcggcct catctccagt ttcgagaaat tctcgtgctt cgaaacagaa
    12961 ataccagccc tggctaaagc gtccatgtag agtaatgacg accgggagat ctgcatcatc
    13021 aaggtaatgg ttcgctttcg cgaatgcgtc gtgataagta atcataatta taataaatat
    13081 tattgttgag tgttatatta ttattcctgt gtgatacatt gagcaaagac gcgttcatct
    13141 tcgtcaaaga tattattaaa tgctggtttt gaaaaaagtt catcggataa gatatagtca
    13201 cgaaataagt atctgctaat attttatgat ttctttttca gaacgtcgaa cgggattttc
    13261 ttatttaaga tatcagaatc ttatgattcg ggtaaaattc taaataaatc aacatgtcat
    13321 taaaatcatc atagccatta gctatttcgg ctaaaataga gactacatgt cttcggtcca
    13381 tctcacttaa ggagtgtagt tccgttgtaa gtttttccat agcttgcact gctaaatttc
    13441 gaacaaggaa ttttctgctg gtaatctcta aaaagatggc atggtttaca atgatttttg
    13501 tttccttttg attattatga acaactgtcc atgatttcgt ttaagaatga agagaaatca
    13561 ctaaacgaac tgaatatatt ttctgtgcca atattatctc taatttcaaa aaagttactt
    13621 ttaatgtcat aagtaaatat tgaaatg
//
LOCUS       DAZFKM010000136        13274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFKM010000136 DAZFKM010000000
VERSION     DAZFKM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..233)
                     /locus_tag="ACHKAY_004113"
     CDS             complement(<1..233)
                     /locus_tag="ACHKAY_004113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702691.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKTQV"
     gene            170..1669
                     /gene="pitB"
                     /locus_tag="ACHKAY_004114"
     CDS             170..1669
                     /gene="pitB"
                     /locus_tag="ACHKAY_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO5702692.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1718..2410)
                     /gene="yghT"
                     /locus_tag="ACHKAY_004115"
     CDS             complement(1718..2410)
                     /gene="yghT"
                     /locus_tag="ACHKAY_004115"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5702693.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            2584..3297
                     /gene="yghS"
                     /locus_tag="ACHKAY_004116"
     CDS             2584..3297
                     /gene="yghS"
                     /locus_tag="ACHKAY_004116"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO5702694.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3329..4087
                     /locus_tag="ACHKAY_004117"
     CDS             3329..4087
                     /locus_tag="ACHKAY_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702695.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4133..5482
                     /locus_tag="ACHKAY_004118"
     CDS             4133..5482
                     /locus_tag="ACHKAY_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO5702696.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5482..6306
                     /locus_tag="ACHKAY_004119"
     CDS             5482..6306
                     /locus_tag="ACHKAY_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702697.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6318..6878
                     /locus_tag="ACHKAY_004120"
     CDS             6318..6878
                     /locus_tag="ACHKAY_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO5702698.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6909..7979
                     /gene="lptF"
                     /locus_tag="ACHKAY_004121"
     CDS             6909..7979
                     /gene="lptF"
                     /locus_tag="ACHKAY_004121"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO5702699.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7976..9055
                     /gene="lptG"
                     /locus_tag="ACHKAY_004122"
     CDS             7976..9055
                     /gene="lptG"
                     /locus_tag="ACHKAY_004122"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO5702700.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9091..10263)
                     /locus_tag="ACHKAY_004123"
     CDS             complement(9091..10263)
                     /locus_tag="ACHKAY_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO5702701.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10263..10511)
                     /locus_tag="ACHKAY_004124"
     CDS             complement(10263..10511)
                     /locus_tag="ACHKAY_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO5702702.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10543..11457)
                     /locus_tag="ACHKAY_004125"
     CDS             complement(10543..11457)
                     /locus_tag="ACHKAY_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5702703.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11454..13142)
                     /locus_tag="ACHKAY_004126"
     CDS             complement(11454..13142)
                     /locus_tag="ACHKAY_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO5702704.1"
                     /translation="MRYADFPTLVDALDYAARSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3185 a         3266 c         3446 g         3377 t
ORIGIN      
        1 aatacctgtg ttttgattat ttctaaaggt tttgaatgaa tgcttattgt ctgatacaca
       61 agaaataaca ctctttttat cgttaaaaaa tgatatttca ctttgcccat gccgttaaag
      121 tcactgataa tgcgtccgtt cgtaaattca aaatggcgta atctaatata tgctaaattt
      181 atttgttggc cttgatatat acacagggct tttgttattg cttgctctgg catttgtgtt
      241 gttctacgaa gcaatcaatg gttttcatga cacggcgaat gcggtggcaa ccgttattta
      301 tactcgtgcc atgcaaccac aacttgctgt ggtgatggcg gcatttttta acttttttgg
      361 cgtgttattg ggcggactta gcgttgccta tgccattgtc catatgttac caaccgattt
      421 gttgctgaat atggggtcaa cccacggtct ggcgatggtc ttttccatgc tgctggcggc
      481 gattatatgg aacctgggga cgtggttctt cggtttaccg gcctccagtt cgcacacctt
      541 gattggcgcg attatcggca tcggtttaac caacgcgctg ttaaccggct catcggtgat
      601 ggatgcgtta aacctgcgtg aagtgaccaa aattttctcc tcgctgattg tttcccctat
      661 cgtcggcctg gtcattgcgg gtggtctgat tttcctgctg cgccgtttct ggagcgggac
      721 gaaaaagcgt gaccgtattc accgcattcc ggaagatcgc aaaaagaaaa aaggcaaacg
      781 taaaccgcca ttctggacgc gtattgcgct gattgtttcc gctgcgggcg tggcgttttc
      841 gcacggcgcg aacgacgggc agaaagggat cggcctggtg atgctggtac tggtggggat
      901 tgcccctgct ggcttcgtcg tcaatatgaa cgcgtccggc tatgaaatta cccgtactcg
      961 cgatgccgtc tccaacttcg aacactatct gcaacagcat cctgaactgc cgcagaagtt
     1021 gattgcgatg gaacctccat tgcctgcggc atcgactgat ggcgcgcaag taacagagtt
     1081 tcactgtcat ccggcaaata cctttgatgc gattgcgcgc gttaaaacga tgctgccagg
     1141 caatatggaa agttacgagc cgttaagcgt gagtcagcgc agccagctgc gccgcattat
     1201 gctgtgcatc tcagatacct ccgcgaagct ggcgaaactg ccaggcgtca gtaaagaaga
     1261 ccagaacctg ctgaaaaaac tgcgcagcga tatgttaagc accattgagt acgctccggt
     1321 gtggatcatc atggcggtag cactggcgct cggcattggc accatgattg gctggcgtcg
     1381 tgtagcgatg accatcggtg agaagattgg taagcgcggc atgacgtatg cgcaaggcat
     1441 ggcggcacaa atgacggcgg cagtgtctat cggtcttgcc agttatattg ggatgcccgt
     1501 ctccacaaca cacgtcctct cgtctgcagt tgcagggacg atggtggtgg acggcggtgg
     1561 gttacagcgt aaaacggtaa ccagcatcct gatggcgtgg gtatttactt taccggcggc
     1621 gatttttctt tctggtgggc tgtactggat agcattgcag ttgatttaac cttcctgaaa
     1681 atgcccggtc ctggcgatcg ggcatttcca tttttggcta gtgataacca cgcgcggtca
     1741 taaaatccat aatcgctttt tctgcatcaa ccaacacctg ttccagtggc tgattggcat
     1801 cgatatcaac cagttgtgca ccaccaaagg ttaactgtgg cgttatggct atctttcttg
     1861 ccagcgactc ccttttgtgg tcaggcttac gcgcgcaggc aacttcaagg tcaacattga
     1921 gtttgatgac cagatcaggc ttatggctcg ccatccagtg aaacgcttta cgttcctggc
     1981 ttgccagcca tgagacaaaa cgaccacctt caacgttagg tgggaacacc gtaccatcgt
     2041 aagcgccagg aatttggtcc tgaggataac ggtcggttag aacaattaac ccgcgacgac
     2101 gacaggcaag catatgacga aagcgcagta agcgacgggc gacaaacgct gtaattacca
     2161 gcgccggaac tggtcctggc aatttttttg ctgttttcac ctgatttcgt tcaattgttt
     2221 tatgtaagga ttttcccatc aacggtaatt ttgtcactgc acgaccgaca tttccggctt
     2281 gttttcctaa atgaactctt tcggcagcac catatttttc gacaacggta ataagatgtt
     2341 cacacaccgt tgacttgcct gaaccatcgc taccaataac ggcaattaat ggaggtgtta
     2401 ttgactgcat gaatatatcc ttaaatttaa atatccatcc aaaatattta tttggttaat
     2461 atgtttttat gaaagcgtaa ttcaggtcaa tgtcacaatt aaccattgtc acaataaggt
     2521 tgaacggata ttctgagcac ggaccacgta atattcaata cattatttac tgccgggttt
     2581 ttcgtgaatc aacgtaatat gtcaataatt aattccacgc cagtgcgtgt tattgccatt
     2641 gtaggatgtg atggttcagg taaatcgacc ctcacggcaa gcctggtaaa tgaactggca
     2701 gcaagaatgc caacagaacg catttatctc gggcaatcgt ccgggcgaat tggcgaatgg
     2761 atttcacagc tccctgttat tggcgcacct tttgggcgtt atctgcgaag taaagcggca
     2821 catgtgcacg aaaagccctc aacaccgcct ggcaatatta ctgcgctggt tatctatctg
     2881 ctttcctgct ggcgggcgta caagtttcgc aaaatgttgt gtaaaagcca gcaaggcttt
     2941 ctgctcatca ccgaccgcta cccgcaggtt gaagtgccgg gttttcgctt tgatggcccg
     3001 caattggcaa aaaccacggg cggtaacggt tggataaaaa tgttaaggca gcgcgagctg
     3061 aagctgtacc aatggatggc atcttatttg cccgtattgt tgattcgtct tggcattgat
     3121 gaacaaaccg cgtttgcgcg taaacctgac caccaactgg cagcgttaca ggaaaaaatc
     3181 gccgttacgc cgcaactgac attcaatggc gcaaagatcc ttgaactgga tgggcgacac
     3241 cccgccgatg aaattctgca agcgtcacta cgcgcaattc acgccgcgct ttcctgatca
     3301 ccaaaatgga taggggatga tttaacgaat ggatgcacta caaactcaaa ctgttaatag
     3361 tacaaccgcg ccgcagccca actacattcc ggggctgatt gcggtggtcg ggtgtgatgg
     3421 caccggtaaa tccacactga ccaccgacct ggtgaaatcg ctgcaacaac actggcaaac
     3481 cgagcggcgc tatctggggc tgctctccgg cgaagacggc gacaaaatca aacgattgcc
     3541 gttggttggc gtctggctgg aacggcgact ggcggccaaa tcctcgaaaa cccaaagcat
     3601 gaaaaccaaa tctccggcgc tatgggcggc ggtgattatg tactgcttct cgctgcgaag
     3661 aatggcgaat ctacgcaagg ttcagcgact ggcgcaaagt ggcgttctgg tggtcagcga
     3721 tcgcttcccg caggctgaaa tttcgggctt ttattatgat ggaccgggga ttggcgtcga
     3781 acgtgcgacc gggaaaatca gcatgtttct ggcgcagcgc gaacggcgtt tataccaaca
     3841 aatggcgcaa tatcgcccgg aattaattat tcgcctgggc attgatattg agactgccat
     3901 ctcccgcaag cctgaccatg actatgccga gctgcaggac aaaatcggcg tcatgtcgac
     3961 gattggctat aacggcacaa aaattcttga gattgattcc agagcgccct acagcgaagt
     4021 gctggaacag gcacagaagg cggtttctct ggttgccatc gtttctgacc gccgaagttt
     4081 aacttaaggc tggcgggata ctttttacta tcaacgataa ggtcaggctg aattggcggg
     4141 ttttaacatc aaacattggt ttgcagatgg cgcgtttcgc accattattc gcaatagcgc
     4201 ctggttaggc tccagtaatg tcgtgagcgc cttgctgggt ctgttggcgc tctcgtgtgc
     4261 cggtaaaggg atgacgcccg ccatgtttgg cgtactggtg attgtgcaat cgtacgccaa
     4321 gtcgatcagc gattttatta agtttcagac atggcaactg gtggttcagt acggaacgcc
     4381 agccttgacc aacaataatc cgcagcaatt tcgcaatgtc gtctcatttt ccttctcgct
     4441 ggatatcgtc agcggcgcgg tggcgattgt cggcggcatt gccttactgc cattcctttc
     4501 ccattcatta ggtctggatg accaaagctt ttggctggcg gcgctgtatt gcacgctcat
     4561 tccttcaatg gcttcctcca cgccgaccgg cattctgcgt gcggtagatc gcttcgattt
     4621 aattgctgta cagcaggcga cgaaaccttt tctgcgcgca gcggggagcg tcgtagcctg
     4681 gtattttgac tttggttttg cgggttttgt tattgcctgg tacgtgtcga atctggttgg
     4741 cggcaccatg tactggtggt ttgcagcgcg cgaattacgc cgcagaaata tccataatgc
     4801 cttcaaattg aatctgtttg agtctgcccg acacatcaaa ggcgcgtgga gttttgtctg
     4861 gtcaaccaac attgcccact ccatctggtc ggcgcgtaac tcgtgcagca cagtgctggt
     4921 ggggatcgtg ttaggacccg ctgccgccgg gttatttaaa atcgccatga cattcttcga
     4981 cgccgccgga acgccagcgg gtttgctggg taaaagtttt tacccagagg tgatgcgttt
     5041 agatccgcgc accaccagac cgtggttgtt aggtgtgaag tccggtttac tggcgggcgg
     5101 aatcggtatt ctggtggcgc tcgcggtgtt tatcgtcggc aagccgctca tttcgctggt
     5161 gtttggcgtt aagtatctcg aagcgtatga cctgattcag gtgatgctgg gcgcaattgt
     5221 gatttcgatg ctgggcttcc ctcaggagtc attgctattg atggcgggaa aacagcgcgc
     5281 ttttctcgtg gcgcaaacca tcgcctcaat cggctacatc gtattgctgt tcatgttctg
     5341 tcatctgttt ggtgtgctgg gagcagcgtt tgcttacttc ggtgggcaat gtctggacgt
     5401 ggcgctctcg ttaattccaa ccttaaaggc attttttcag cgccattcct tgctttataa
     5461 cgcagccgga gagaaatcct gatgatgaga aaatatttcc cgcttgaggc gagcgagcgt
     5521 ctatttgttg cgattgaaga agatgatgtc gttgatgcac aggttagttt gccgccgact
     5581 attgcactaa gttgtaccac cgaaatcatt catgataatt acgcgttatg cctgcaattc
     5641 tggctcaatg gcgttgatcg tcaggaatta ctgcgtttag ttcgcaaaca agccaaaggc
     5701 gatgagctga ctgctgatga gcgcaaacaa tttaaataca tgcgagcgcg ctacaagcac
     5761 ctgcgttttg cgcaacggct gtatttaaag aagcatcagg ccggattttt attcggcaaa
     5821 acgaccgttt ttctggggcg ttttcaggac ggctttcgta acggtaagaa aaatattgtg
     5881 tcgtattacg gcaacctgtt acggatttat ttaagctctc cggtctggtc gttggtgaac
     5941 tattcattac gccatagcca gttagaaagc gtaagcagtt ttattgccta tcggcaaaag
     6001 caaatgcata ccctgaaaga gattattgcc aaaccgcgac taaccggcag ggaatttcat
     6061 gatgtgcgaa aaatcatcag ccaacaggtt tcttattacg acaccttaag atcgcttgat
     6121 cctgaaaata aagaggcgtt acagatttcc cgtttcctcg ccgccattaa tggcctgatg
     6181 ggagataaac acgacgacat ggtggcggat gatatggaaa accgccagtc gtatgatgca
     6241 ccgttggcgc tggatagcga tattcgtcag cgcttagagc tgctgatttc gcgttttccg
     6301 ctatagccgg agcaatgatg tcatctcgtt tgattattgc gttgattatc atgctcttag
     6361 ccccgggtgt gcaggcacac aatttcgtca ctggcaaaac ggttacccct gtttatatcc
     6421 aggaaggcgg cgagctgctt ctgaatagcg acgacgaaat tcactaccaa aagtggaaca
     6481 gcacacaatt agcaggaaaa gtccgcatta ttcagtatat cgccgggcgt aagtcggcga
     6541 agaaaaagaa ctcactgtta attaaagcgg ttgaagcggc aaactttccg caggatcgtt
     6601 ttcagcccac caccattgtg aacaccgatg atgccatctt tggtactggc tattttgttg
     6661 ttggcaaaat cgaaaaaaat aaacgccgct atccgtgggc gcaattcatt attgacggca
     6721 atggacaggg acgcgttgcc tggcgcttgc cggagcagag ttcaaccatt ctggtgctga
     6781 ataaagccgg gcaaattcag tgggcaaagg acggcagcct gacgccggaa gaggtagatc
     6841 atgtcattgc gctggcgcag aaactgatca atgaataaaa aattcaaagg attgagggcg
     6901 ttagtgagat gaagctggtt gaacactaca tcatgcgtgg tacgcgccgt ctggtgctga
     6961 ttatcgtcgg ttttctgatc tttattttcg ccagctactc cgcacagcgt tatctgaccg
     7021 aagcggcaaa tgggacatta gctcttgatg ttgtgctgga tatcgttttc tacaaagtgc
     7081 tgattgcact ggagatgttg ttacctgttg gtctgtatgt gtcagttggc gtgacgctag
     7141 ggcagatgta taccgactcg gaaattaccg ctatctctgc ggcggggggc agtccgggac
     7201 gcttgtacaa agccgttctt tatctggcga taccgctaag tatttttgtc acccttctgt
     7261 cgatgtatgg tcgtccgtgg gcttatacgc agatttatca actggagcag cagtcgcagt
     7321 cggagctgga tgttcgccag ttgcgggcaa agaaatttaa cactaacgat aacggacgaa
     7381 tgatcctttc gcagacggtt gatcaggata ataatcgcct gactgacgcg ctgatttata
     7441 cttctactgc caatcgaacc cgcattttcc gcgcccgttc ggttgatgtg gttgacccat
     7501 cacccgagaa accgaccgtt atgttgcaca acggcactgc ctatcttctc gatcatcagg
     7561 ggcgtgacga caacgaacag atctaccgta atctgcaatt acatctgaat ccgctggatc
     7621 aaagccctaa cgtcaaacgc aaagcaaaat cggtcacgga gctggcgcgc tccgcctttc
     7681 ctgccgatca tgccgaactg caatggcgac aaagccgtgg cctgacagca ttgttgatgg
     7741 cgctgctggc catttcatta agtcgggtaa aaccgcggca agggcgattt tcaacattat
     7801 tgccactgac gttgctgttt attgccattt tttatggcgg cgacgtctgc cgtacgctgg
     7861 tggctaacgg cgcgattccc ctcattcctg gtttgtggtt agtacccgga ctcatgctaa
     7921 tgggcctgct gatgctggtc gcacgcgact tctctttgct gcagaaattt tcccgatgaa
     7981 tgttttcagt cgctatttaa tccgtcatct ctttctcggt tttgccgccg ccgcagggct
     8041 attgctgccg ctttttacca ccttcaacct gattaacgaa ctggatgatg tcagcccggg
     8101 cggttatcgc tggactcagg cggtgctggt ggtgctaatg accttaccgc gcacactggt
     8161 cgaactttcg ccgtttatcg cgttattggg tggtattgtt ggactggggc agttatcgaa
     8221 aaacagtgag cttaccgcca ttcgcagcac ggggttttct atcttccgta ttgcactggt
     8281 ggtgctggtt gccgggatat tgtggactgt ttcgttaggc gcgatagatg agtgggtggc
     8341 gtcgccattg cagcaacagg cgttgcaaat caaatcgacc gccaccgcgt tgggggagga
     8401 cgatgacatt accggcaata tgctgtgggc caggcgtggt aatgaatttg tgacggtgaa
     8461 atcgctgaac gagcagggcc agccggtggg cgtggagata tttcattatc gcgacgatct
     8521 ctcgcttgaa tcctacattt atgcacgcag tgccaccatt aaagacgaca aaacgtgggt
     8581 cctgcatggt gtgaatcata aaaaatggct gaatggcaaa gaaacgctgg aaacattaga
     8641 taatcttgcc tggcaatcgg ccttcaccag tatgaatctt gaagagttat caatgccggg
     8701 gaatactttt tctgtccgtc agcttaatca ttacatccat tatttgcagg aaaccgggca
     8761 acccagcagc gaataccgcc ttgcactgtg ggaaaaactg gggcaaccca tcctgaccct
     8821 ggcgatgatt ttgctggctg tgccgtttac ctttactgcc ccgcgttcgc ctgggatggg
     8881 tagccgtctc gctgtaggcg tcatcgtggg cttactcacc tggatcagct atcaaatcat
     8941 ggttaatctg ggattgctat ttgcgttaag cgcacctgtt accgcgctcg gtttaccggt
     9001 agcgtttgtg ttggtggcgt tgagcctggt gtattggtat gacagacaac attaaacccg
     9061 ccgtggcgca aacaccacgg cgggctcaca ttactgcttc agagtagcaa atgcctgaat
     9121 gatggcatcg atttgctcac gactgtgggc agcatttacg ctacagcgca gcagcgtgat
     9181 gcctgcaggc gcggcaggcg gcaggataag attcacatac acgcccagcg agataagctc
     9241 gcgccagatg cgtaaacctt cctctttcga accgattatt actggcacta ccgggctaat
     9301 atgtgaactt aactcataac ccagtttttg cagaccgtca tacagatggt tggcgttatc
     9361 catcagcttt tgccgcagct ctggttgtgt ggcaatagtt cttaacgacg agcgcacggt
     9421 cgcaatgcag gacggagaag gagaggcggt aaaaatatac gggcggctgc tgtagcgcag
     9481 cacttccatc gcttccgaac cgacagcaaa accgccaatc gaggccagac ttttactgaa
     9541 agtaccaacg ataatatcca catcatcttc aacgccgaca gcttcagcca gtccgcgccc
     9601 cgttgcgcct aacacgccaa acgaatgcgc ttcatcgaca atcaaataac caccgagacg
     9661 acgtttgata tcaacaattt ccgccagcgg cgcaacgtca ccgagcatgc tgtagatgcc
     9721 ctcaacgatg ataatcgcct ctttcgcccg ttcaccgagg cgaaccatgc gccgttccag
     9781 atctttagca tcgttatggc ggaaacgaat aatttccgcg ccaccgagag aacaggcgtc
     9841 ataaatactg gcgtggctgt cggcatccag taacaccacg gcgttatggt cggcaagtgc
     9901 gctgatgacg cctaaattag cggtataacc ggttgaaaaa acgatagcgg tagggcgatt
     9961 gaaaaactcg gcaatctctt tctctaacgc caggtgcggc gcataactgc cgtttgccat
    10021 acgagaaccg gtggtgccgg tgccttgggc cgctaacgct gcctgacctt cgctgatggc
    10081 atcatgatta aaggttaaac cgagataatt attggtcccg gccagaatca cttttttatt
    10141 gccgatccgg ccttcggtgg cggaatacac ttcatcaata caggtgccaa acggtgttaa
    10201 cccgttgtca ctaaattgca gtcgctcgcc agcaaggcgt gcatatttat cgtatagccc
    10261 cattatttat tctccagcca tggaagtaga gtttccatta attgcgcagg ggttttgaca
    10321 tccagcagaa tattaatagg aatggaaata tcgaatctgt cttccagcat cattaagaga
    10381 tccatcactt tgatcgactc caggccaaga tcgttgacga gatcgctgtc gggcttaatt
    10441 tccacaccgt tttcgaccaa atcctgtaaa caggaaagga tgtaatccat tactatttca
    10501 cgatttacca tagaaacgtt aacacacctt taaacaagac aatcaaaata gcccgttgcg
    10561 taatgcgtgt tccaggctaa tcccgggaaa ccaattaata tcttcagaaa tacggttatt
    10621 acttgccgac cagtcggcgt gggttaattc acgaattttc gagcgggtca gcataggttc
    10681 tttacctgcc aggcgactca acgcggtact gatatccgca aggcaggtga gtaccggcag
    10741 aggaatacca accattcgca cggaaccgca acggacgttg gcggcaagtt gctgtatgcg
    10801 ttgccagtca tagccgccag cgacgctatc gcataattca taggtttgcg tctgtacagt
    10861 ttcggcgctt aaccactgac ccactgcttg cgcaaaatcg gtgacgtgca ggaaagagag
    10921 ctgtgtatct ggtgcaccaa gtcgtggcag caggccgcgc agcatccagt caaacagcgg
    10981 ttttaactct ttatcgccgg gaccatacac ggctgtcggg cgaaaaacgc ccagcgtaat
    11041 ttcgtcagcc attgcagtca gccgttgttc ggcgatgtgt ttggaatttg cgtaccagga
    11101 gagctcggga tggcgcgccg ccagcgaaga gataaacaga aaacgttggc aaaagcagct
    11161 ctcttttgct gcttgcatca ggcgcaggct gccgtcaacg ttacagcggg tgaaaatctc
    11221 ttctttgtgc ccgcgcactt gcccggcgca atggaccact gcgctggctc cggcaaccag
    11281 cttgctaagt gaatgagtat cttccagcga accgcgcacc caggtgagat tatcattgac
    11341 gtgagcgcgg gcagtacgcg tcaatgcgcg aacgtgaaag ccgcgggcga gcaggttatc
    11401 gataatatat ttaccgataa acccggtagc gcccgtcacc gcgacagttt gattcatgcc
    11461 agggattcct gcacattaag actggcagca taagccttct gataacgttt tttcgcttcc
    11521 gcacgggcag gcttgccgga ggacgttcgg ggaatactgt ggggcggcaa cagatcgata
    11581 gccgcggtca cgccaaattc gctttggatc cgtgccgcca gcgcgtggat aagctgcccg
    11641 cgacgttctt cgtcgctgat ccgacactgg atctgcaaaa tgattttttc ctgggcggta
    11701 acaaaagcaa tcgcatcgcc agaatgaatt tccggttctt gttccgcaat atattcaata
    11761 tcctgcggcc agatattacg gccacgaata ataatcagat ctttaatgcg tccggtgaca
    11821 tacagataac cgtccagcag ataaccgagg tcgccggtgt ctaaccagcc cgtcgcggca
    11881 atctcgtctt gcgaaacctg gtcgccaaag taaccgctca tcaggctggg gccggagatg
    11941 caaatatggc ctaccacacg ttccgcgacc ggcatacctg cttcattgcg gatttcaatg
    12001 ccatgttccg gcaacgcttt gccgcagttg acgaaagtcg atacggcgcg tgtctctgca
    12061 cccggcgcga cggctttacc ctgatattcg aggatgtcgc gatccacttc gttaaccaca
    12121 accccggagg cttcatcaga gaagctgaca gccagcgcat tttctgccag tccgtagcac
    12181 ggcatgaaag ttttattgtc aaagttaacc tgacggaaac attcagcgaa ttgatggagt
    12241 tgttctgcgg agatgggttc tgcaccaata ccagcgacgc gccagcagga aagatccagt
    12301 tcagcgagat ctttttcatt cacgcggcgc tggcacaatt catagccaaa cggcggcgca
    12361 acggaaacgg tgccgcgatt tttactgatc aatttaagcc attgcagagg acgcatggca
    12421 aaatcctgag tgcgcaaata atctactgaa agctgcgtgg cgacgggggg caggagaaag
    12481 ccgaccagtc ccatatcatg gtagaaaggc agccaggaga cgcagcggtc gccagggcgt
    12541 aatttaatgc cgtcgtggct tatagcacgt agattagcca tcacttcgcg atgggtgata
    12601 atgacgccac ggggaaaacg ggtgctgccg gaggtgtact ggaggtaggc gatatcgttc
    12661 ggaactggac gctggagcgc aacatcggct tccggtaatg ccttaaacca ggcgtggctt
    12721 aaaacatgta attcggggtt gttatgcgtc gcggcattga ccagtggcaa ccactcatca
    12781 ccagtgataa tggctgcggg ctggcaactt gccagtaaac cctggagttt ggcgctccag
    12841 gaatcccgct gaccgacgcc cattggaatc gccaacggga cggcgactaa gccagcatac
    12901 tgacaggcaa aaaaagcctc tacaaacccg ctactcgttt cggcaatcag cgccacgcgt
    12961 tcgccttttt tcaggttcag cgataacaac cgcttcgcac cagcttcggc acgcgttttt
    13021 aatgtctgat attccagttg atcttcaagt tggcaacgtc tgtcataaaa attcattccg
    13081 gcgctactcc gagcggcgta gtccaaagca tcaaccagcg ttggaaaatc ggcatagcgc
    13141 ataggtaggg aatgcgtaaa gattttatta gacatataca ccacgaaaaa attggatatc
    13201 aaaaataaaa tgacaataat gcggtggatt atataggcat aaaagataaa tgagatagta
    13261 ttttcatcgg tgtg
//
LOCUS       DAZFKM010000137        13115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFKM010000137 DAZFKM010000000
VERSION     DAZFKM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..1872)
                     /locus_tag="ACHKAY_004127"
     CDS             complement(4..1872)
                     /locus_tag="ACHKAY_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO5702705.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            complement(1961..2770)
                     /locus_tag="ACHKAY_004128"
     CDS             complement(1961..2770)
                     /locus_tag="ACHKAY_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5702706.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQ
                     NEREFIYNVSGFDNLHQKAKEMLSSAECEIYLNTDFDLHFLKNEICEALNRHVRVIVF
                     SFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLL
                     INIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            complement(3177..4304)
                     /locus_tag="ACHKAY_004129"
     CDS             complement(3177..4304)
                     /locus_tag="ACHKAY_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5702707.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLYTSFCHIFDFYTEENLTNYL"
     gene            complement(4387..4875)
                     /locus_tag="ACHKAY_004130"
     CDS             complement(4387..4875)
                     /locus_tag="ACHKAY_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO5702708.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            complement(4966..5922)
                     /locus_tag="ACHKAY_004131"
     CDS             complement(4966..5922)
                     /locus_tag="ACHKAY_004131"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO5702709.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(5941..6978)
                     /locus_tag="ACHKAY_004132"
     CDS             complement(5941..6978)
                     /locus_tag="ACHKAY_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO5702710.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            7251..8483
                     /locus_tag="ACHKAY_004133"
     CDS             7251..8483
                     /locus_tag="ACHKAY_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5702711.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPEALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            8486..9775
                     /locus_tag="ACHKAY_004134"
     CDS             8486..9775
                     /locus_tag="ACHKAY_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO5702712.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFIL
                     ALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDF
                     LSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITL
                     PMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNF
                     QQYHIASVISIIIFIFLSVIAIWQFRRMKSFKDDVGM"
     gene            9778..10611
                     /locus_tag="ACHKAY_004135"
     CDS             9778..10611
                     /locus_tag="ACHKAY_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO5702713.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            10631..12430
                     /locus_tag="ACHKAY_004136"
     CDS             10631..12430
                     /locus_tag="ACHKAY_004136"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO5702714.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            12452..>13115
                     /locus_tag="ACHKAY_004137"
     CDS             12452..>13115
                     /locus_tag="ACHKAY_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO5702715.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGTD
                     NPI"
BASE COUNT         3924 a         2432 c         2503 g         4256 t
ORIGIN      
        1 atattaactc tctatattgg agaaagttac tttgcgaaga atggtttgac caaatggtat
       61 aagttttctg actattttag tcttgtttaa ataaaaacag ccttcaatgt attgtttgcc
      121 ctctggtgag gttgaatatg aaaaagattc catatcagca taagagaact ggatagattc
      181 atcagctcta ctacagggta tgtatcctga gtcaaaatat ttcccgttca gcagagtttt
      241 tttgattaaa atatcgctct caagctcaat tgcatataaa acgggacctc tggaaatata
      301 agtgtcacca cataagtcag tattaaactg tatatcagct ataaatttaa tgtcaatcac
      361 atcatgatgt tgccatgttc tatctaaaat aataagtgag ttttgagtat catttttagt
      421 aaaagtaaca ccattaataa tcatcatttt tgcccagccg gggtaacgga aactgagagc
      481 aaaacgaaca gatgactcag gctcaatgag acattttacg cttaaatcat gagggtattc
      541 agttaattgt gtaattttaa ttgcaacatt attaaatgtg ccataaaact cacacgggcc
      601 ataaaataat gcagtaaaac cattgttatt ttcttgaaac atattttcga caaaagccgg
      661 tatggttctt gctgtattcg gtacgcagca gacagcagcc tcctcatgag taggggagta
      721 tttatatctg gtattaaact gattactgtc cggatgtttc ctttcatttg cttcataaca
      781 attatcagtc ttacagtaca ttatcgcgtg aagtatggga tgctgcatgc caaaactagc
      841 attatagaat agccactcag catcatcggc cagaccaata ttagtggtca gttttattaa
      901 cataacataa gtgctaaata gttcatgaac agaacaatat tcatagcctg tataagtggc
      961 attagctgtg cgtcctgtaa tccactcatc accaatggga gcgccagatg gtgtaagata
     1021 atatttcaac tgggataaaa aaatatctaa tgcagtttga tactgcggcc gttggctcgc
     1081 tgcgatggct aatgccctaa tatgctcata cgtatgtacg ccatgacctt taagaaggta
     1141 accagggtct atgacatttt tatattgcag atcttgctcc gacacgcatc ctgcggaata
     1201 atcttcatac agccacaagc aatatgagag atattcaatg ttattggtta tttcataaag
     1261 gcggtagaaa acatctgtta ttgttaatcc gtggctatgt ccagcaaaac cctgttccac
     1321 aacgaaagga tgtgacgttt ctttagggta gccagccata atgcattgtg cggcttgggt
     1381 aagagaagtt aataaactct cattacgggt ggactcataa catccaagca caaaacgcaa
     1441 ggcagtagct tgtgcccaga actctccatt ttctgcttta cagttaaatt ttaatgatga
     1501 atcataaatt ccaatatagc catcatcatc ttgcgtatcg ataagttcca aacataaatc
     1561 gctcgctttt tcctgccatt ctttattatc taacaagtag gcactgcggc aatatccatc
     1621 cttccaattc gattgagttt cactatccca ccaataatat tgcatcatgt tatcatcggg
     1681 attattctgt tttttaataa cgccatcttc agcagataca gtgcgtggag ttatacgatc
     1741 tcttccatat attttatgct ccgtaataag atggggaact aatttatcca gttctcctat
     1801 atgaccgttc atataccatt ccatcttatt ctttaaccaa ccagtgggct ttatatcgtt
     1861 aaaaaaaatc ataaatatac ctttgcataa aaaagtttag acgttataaa agacaaatag
     1921 attgtgatcg cctacagacg atcacaatga aaggaaaata ttaacggttt ttgctatcac
     1981 tcaaatgttt ttcatcctgc accatatcat tgccagattc atgcaaagta tttattaatg
     2041 cattggcatc tggatattgt tgctgatact tggttagata tatatcacta tggatatgtt
     2101 cggcaattat attgataagc aagcgattat tcgagtataa ccccagtgat tcagctcgtt
     2161 gtgaaaaaac cattactgtc ttcatatcaa caacaagcat aaaacggtga gaagggtaat
     2221 attcttcttc atggttaaag cggtaataca gttctacacg tgggtcggga gtaataagtt
     2281 tattaaatga aaaaacaata actcgaacat gtcgattcag agcctcacaa atttcatttt
     2341 tcaaaaaatg taagtcaaaa tccgtgttga ggtaaatttc acactctgca gaagaaagca
     2401 tctctttagc tttctgatgt aagttatcga aaccagagac attataaatg aattcacgtt
     2461 cattctgaaa catcatctgt gataattctt tttttagaat ttttatattg tcaatagtgc
     2521 ttttttcaat ttgtgaaagt atgagttcag gagattttgc ttcatattct ttagtttcac
     2581 catcgacaag aaagatataa ccgttcttgt aaagattatc aatagatgag tatacagacg
     2641 aacgagaaag tgaaatttct tttgctatct tgtaaccact gctacgaccg tttttcaaca
     2701 gactgatgta aacctgtgca tcggtgcgag tgaaaccaaa atttatcaat ttatttacca
     2761 aatgtatcat agacgcgctc ctgaaatgaa aatgaaatct taccaatttt aacattatta
     2821 attaacattt tcgaaaaaat tgctgagcta aaatgtggat ttatcgtaat tcatcctcaa
     2881 gctggttaca ggtctacagt acgtaagcat tgtgaactgc gaatctgaat gccctccaac
     2941 tatggtcaga agacatgctc tgacctaaat agtaggctca ctgagtaagc ggtgttatca
     3001 acggtttatt catatatgct cttttttatg gatggaattt tgtaactcaa ttaccttgat
     3061 aagaatcagt tcgtggagtt aatgacattt accaatagac agttcgagca gtaagaagcc
     3121 ttatggaatt gtaaggttta ataccgttat tcaacctggg gttattgtca tatcaatcac
     3181 aaataattag ttagattttc ttcagtgtaa aaatcaaata tatgacagaa gcttgtataa
     3241 aggttgaaat acaaattttt accaatatcg gctgtagtaa ttgattgatc gttgactttt
     3301 gcaataaact ctttgttgtt aacggtaaag taaagatatt tttcactgcc catattttca
     3361 ataatactta atgcaccagt gactgtattc atttctttgc aattcaaact caaagagata
     3421 tgttctggac gaataccaaa acaaacgggt ttatctatat acgctttgag tactgattgt
     3481 ttattctcag gtatgtacaa ccgaatattt ttggcaatgt caatatagag tttaccgtct
     3541 tcctcacgta caaccgcatc tatcaaattc attgacgggg aaccaataaa gccagcaacg
     3601 aatctgttag cagggtggtt atacagatta actggtgtgt cgacctgcat tattcgcccc
     3661 tgattaagca cacaaatacg atcaccgagg gtcaatgctt caatttgatc gtgtgtaaca
     3721 taaatcatgg ttgctggatg accctcatcc ttgagagact gatgcaactg agcaattttg
     3781 actcgcattg aaacacgaag ttttgcatca aggttagata gtggctcatc aaataagaaa
     3841 acatctggct tacggacaat tgcacggcct accgcgacac gctgacgctg accacccgac
     3901 atttctttcg gttttcggta aagtaaatcg ctaatttcaa gtttttctgc aacttcttcg
     3961 acaattttct taatctcttc ttttggcctt ttttgcattt ttaaaccaaa agccatattg
     4021 tcaaatacag tcttatgggg atacagcgca tagttctgga acaccattgc aatgctgcga
     4081 tctttaggcg gaagggtatt aacaaggcgg tcgctaatgt aaatttcacc acctgatacg
     4141 tcttcaagac ctgcaatcat gcgtaatgta gttgatttgg cacacccaga tggtccaaca
     4201 aaaaccatga attcaccatc gtttatatcg agatcaatgc cgtgaacagc tttgaatcca
     4261 ttgtcataaa ttttttcaac tttgcgtaga attaccgcgg ccataatctg ctctcattaa
     4321 caagaatttt ggaatactga tactccacta ttcaacgaat gttgaatagt ggagtgctaa
     4381 aaataactat ttacatggtg ctaccatggc ggctattgcc tttttatcag gaccaatttg
     4441 aatcgtccaa acatactttc catcggcagg aataacgact tttgtatttt tatccatgcc
     4501 tcctttcttg agtgttttag cctgatttaa tggcaattca agacctgtta cagtgaactg
     4561 cgggttccag ttcatagtcg caaattgcat tgttaccgca cctgcttttt cagaataaat
     4621 aagctgataa ataccatctc ccttataatg cattttacgc tctggcttca ttaaccaata
     4681 gccttccggg agggttccgg aaatgtaaag acccgcagca attggccctc tttcatttac
     4741 atcagggagg ttacattctg aatagcgagg atccgctttc acgacacctt cctgactgcc
     4801 agagggtgaa ttgccatcaa cgcatcctga aagaagacca gtcaacacag ccgcaataag
     4861 caatttattt ttcatttaaa ctctcaataa aagtagtata gcttagttaa gctatttcta
     4921 tgtcagtgtt aatgttaatg tatataaata atacttctca acgactcact gtttatatac
     4981 accatgctgt ttagttagtt tattaaggac agcgagattt ttgttgtatt ctcgcgtatt
     5041 tttggcaggg taaccaaaaa ggtcactctt ttcaggacaa gaatggctta cacctgattt
     5101 agccttaatt accgaataac taccaattga tatgtgaccg gcgattccga cttgaccatg
     5161 tgtaataaca tggtcgccta aaactacgtg tcctgaaaaa ccgcattgtg aaacaatcag
     5221 gcatttatta ccgattatgc agtcatgtcc aatctgcact tgattatcaa tacgagtgcc
     5281 ttggcctata atcgtatctc caagtgtccc tcggtcaatg gtgttgttac aaccgatttc
     5341 aacatcatca cctatgatta ctctaccgat gctatcaact cggacatatg aatcacgttc
     5401 atccgacata tattcaaaac tataattacc gatggagtta ttagaatcta tgataacgtc
     5461 attgccaata atagtacctt ctttaatcac tgtattcgca tgaatggcaa cattatcacc
     5521 tatggtcaca cagttcataa ttttaacacc aggcataaaa tggcagttca tacctatctg
     5581 gcagtgttta ccaatgtata catcaggtat cgttgaagtg ttttcttgct caaacaaacg
     5641 atacttatgt accttaaaat atcttaaaac tttgttaaga ttattcacgt tcaggtaatc
     5701 gactacaatc tttgccttag cattagaact aatgatctct tccggtccga taattatatc
     5761 cgcaccagtc gttggtatat gcaacaaatc tccaggtgtc ataacaatcg agagtccacc
     5821 aactaaagga ctatggattg gccgaatggt atcaaccctg agtgatggat caccatataa
     5881 agttccttgc atcaacgttt taatatctag tgcgctgaac ataggtttcc ctctgtttaa
     5941 tcaaaagcga tagaaagcat gaacataaat attatcctga ttgagcacat tatatttttc
     6001 ttcatctctt ggatcataaa agtttttgtg ctctttttcc caggcatgtt tatattcaat
     6061 cttggctatc cagtcgtcac ctattttttg ttcataacct agagtggctt ttcgaattgt
     6121 tttgaaatag gttctgtttg cattgtattg caaactatct gcgttttctg ttttcccgtg
     6181 tccgaagcgg gtgcgtagat acagattacc tgcgttttca aaagcatatt tgataattgg
     6241 ctcgtagtaa gtttcggtat aaatactacg atcatttatt ttaccgtgat taaattcatc
     6301 gttatgcttt ttctggtaga aaaattcaat accaaactcc catgcaccaa aacgtttata
     6361 gggctgaaat agtatacttt gaccttcttc ttcgcgtacg ccccaggagc tcttatcatt
     6421 tcgactgtat aagtattcaa cgtttgaata aaaacctgcg ttccagtcag agttaaagta
     6481 cgtcaacgta gggcgaaaac tgttgtcaac agttacagta tgcaagcctg taacataagg
     6541 gctatctata tttccgtcgg tatacatcag gtcgtatacc agactgaagt cgaaaccgtt
     6601 accaatagtg aacgttttgt ttagtgatat atagttttgc agacttgttt cattttcgtg
     6661 atagcctttt tcttcttgcc ggcgatcgat agacttaagg ccatagattg ctgcaaattg
     6721 ccaattaggt tggctgaaat aggcctggaa aatctcgtgt gttttagtat attcctttac
     6781 tgttttacct aaatagttat cagtgctttt ggtctcatat tcaaatgtag taccaacatg
     6841 tccattaaag cccgcttgtt cttctgacca gtcagtgtcg gaggtaaagc cgcgatcgtt
     6901 tgccgcaaag gctcctgttg agcctaaaag gactgttgca attgaagccg ataagacatt
     6961 cttttttatt aaaaccataa atgagttcct tccaaaatat caatttaaga tatgatttct
     7021 gttattccat atagtcgtac ctcatgtact cctaaacact ctgcggcaat aaaatatttt
     7081 tttcctcaac aaattactgc aaatgagata tgcatcacta tttgcagtta ttcatcatta
     7141 aaatcttatt tgacagttga ttggccttga tttgtgttga taatggcgtg aatgcttatg
     7201 taggcgtaca gcatgtacta cttaaagata ttattcacga ggtaaatata atgagacttt
     7261 caaatatcgt cacagttata attcttgcaa tttccagtac gctaacaccc caggcgatgg
     7321 cagaaaaatt gattcctgaa actgatgctg agttattaat ctggtctgat gccacaagcg
     7381 cggaatatat gaaatatgca gcaacggaat tcaataaaga ttttggctac aaggtcaagt
     7441 ttacatttcg caatatagcg ccaatggatg cagcatcaag aattatgcag gatgggggta
     7501 cgactcgtgt agctgatgta gctgaaattg aacatgatac cctggggcgg ttagtcgttg
     7561 ctggcggggt tatggaaaac atggtctcag ctgagcggat taaaaaaaca tttattccag
     7621 gcgcagtatc ggcagctaca tataataaca tcagctatgg ttttcctgta agtttcgcaa
     7681 cgctggcgct tttctataat aaggatttgt taaacaccgc accaaaaaca ttcgaagaaa
     7741 tcaatacttt cagtgaaaag tttaataatt catccgagca taaatatgct ctgctatggg
     7801 atgtacaaaa ttattatgtt tcacgtatgt ttattacctt gtatggtgcc aacgaattcg
     7861 gtaaaaccgg taacgatcct gaagctctag gcatcgcttc atctgaagcg aagaaagggc
     7921 tagagacaat gaaacgctta aagaaagcga atccttctaa tcctcttgat atgggtaatc
     7981 cacaagttct aagaggtctg tttaatgaag gaaaagttgc tgctgtaatc gacggacctt
     8041 ggtccataca aggttacatt gacagcggaa tcaattttgg cgtgacacgc atcccaacat
     8101 tagatggtca tcagcctcgc acattttcaa cagtacgact ggccgttgta agctcattta
     8161 ccgaatatcc tcatgccgct gaattattcg ctgactacct gacaacggat aagatgttga
     8221 tgaagcgtta tgaaatgacg aatctcatcc caccaatcga ttcacttatg aacaaaatta
     8281 gccagacagg tagtgaagct ataaaagcaa ttattgctca agctaattat tctgacgcaa
     8341 tgccatcaat accggaaatg tcttatctat ggtctccaat gactaatgct attttggcta
     8401 cctgggttga gaataaaaca ccggatgaag ttttaaatca tgcacaaaca attattgaag
     8461 aacaactttc gcttcaggag taattgtgaa tacacaacaa ctgaaaatga agagtgcacc
     8521 tgtgctacct atttcctgtt tgattatggg cgggactcag ttatcacgtc attattatgt
     8581 gaaaggcggg atattctttg caatacaagt atgctttttg ctttatcttt ccgatattgt
     8641 gcatacatta ataggtttgt tcacattagg tgatgtcgcg caaattcgaa aagggttgac
     8701 tgttattcag ggtgacaatt ctatatttat gcttgtagag ggcgtgatag cgaccattat
     8761 tgttggctta ttcgccacta tttacatcct taatattaaa gatgcgcgga actcatctta
     8821 ttgccatctg acttttaaac aacagttata taagctctat gaggataaat ttgcatttat
     8881 agtcctaacg cccgcatttt tagcgagtat agcattcatt gttttaccta ttgtaattac
     8941 agttcttgta tcgtttacaa actatgctgc gccaaatcac atcccaccaa agaacctggt
     9001 tgattgggtt gggattaaaa atttcattat gttgtttaaa tttaaaatat ggtctgatac
     9061 gtttctaggt gtcgctctct ggacatttat ttgggcaatt tgcgcgacga tttttacctt
     9121 cagttttggc tttattctgg ctttagcact ggctaaaaaa aatatacgtt tcgcaaaagt
     9181 ctggcgattg atatttatac tgccatatgc catacccaca tttgtaacgt tacttatttt
     9241 ccgtcttctt ttaaatggaa ttggtccggt taactcgttt ctgaacagta tcggaatgaa
     9301 ttcagttgat tttctgtcgg ttccattaaa tgcaaaaata acagtaattg ccgtttgtgt
     9361 ttgggtaggg gcaccgtatt ttatgctatt gatcgcgggt gcattaacaa acatatcccg
     9421 agatttgtat gaggctagtg aggtcgatgg cgcaactaaa tggcaacagt ttaaggaaat
     9481 tacattgcca atggtgttac atcaggttgc gccaactctg gtaatgacat ttgcatataa
     9541 tttcaataat tttggtgcaa tttatcttct gactgaaggt ggtccgacta atccggaata
     9601 cagattcgct gggcataccg atatttttat tacctggatt tataaactca cattgaattt
     9661 ccagcaatat catatagctt ctgtaatctc catcataata tttatcttct tatcggttat
     9721 agcaatatgg caattcagac ggatgaaatc atttaaagat gatgtaggga tgtgacaatg
     9781 gataaatttt tacgtaaaat atcaacgctc ttagtttatc tttttttaat ctgcaactcc
     9841 attcttgtgc ttgggccagt gatatggacg ataatggcat cttttaaaaa agggaataat
     9901 ctatttagtt caacatttag tggcatcgag tttacatttg accactacat aacgttgttc
     9961 acggatacgc catatatgca gtggtatttg aacactttca ttcttgctac cgctaacatg
    10021 ttaatttcac tgttcgttgt gacgatgacc gcctacgttt tttcaaggta tcgatttcgt
    10081 ggaaagcgta atactttaat gagtattctt gtattgcaaa tgtttcctgc atttttatca
    10141 atgactgcaa tttatatcct gctatcaaag gccaatttga tagacacata tactgggttg
    10201 cttctggttt atgtaaccgg ttcattgccc ttcatgacgt ggttggtaaa aggttatttt
    10261 gatgctattc caacttcttt ggatgaggcc gctaaaatag atggagcagg gcatctgact
    10321 atttttattc agataatatt gccattggct aagccgattc tcgttttcgt tggattagtt
    10381 ttgttcactg gaccatggat ggacttcatc ttgccaacgt tggtgcttcg tagtgaggat
    10441 aaaatgaccc tggcgattgg aatttttagt tggattgctt ctaattcagc agagaattat
    10501 acgctttttg ctgctggggc gcttttagtt gcagtaccta tcacattgtt atttgtattc
    10561 acacagaagc atataactac tggacttgtt agcggtgccg ttaaggaata atttttaggg
    10621 attagtattt atgattacag ttagttcgtt aaaacactca gcaaaaagtg aagatagcta
    10681 tgcctatgat aacgaaactt tgcatgtacg gttacgcaca ttaagaggag aagttgataa
    10741 agttattctg tggataggcg atccatataa ttgggctgaa ggtggtcttg acggcggtaa
    10801 catggccggg acagaagcgt tcggctggat tggcggcaac gagatcgaga tggaacaaga
    10861 agctgtcacc gaatttcatg atcactggtt tgcagtgttc aaacctcaaa aacgccgttg
    10921 ccgttacggt tttatacttt ttgggaaaga gggagagaag tttctttttg gtgagaaaag
    10981 gtgtgttgat atcagttcac cggactgcga agaacgagag ttaagccgct taaataactt
    11041 tttttgcttc ccttatctta ataaaattga cgttctaaat accccctcct gggtaaaaaa
    11101 tacggtatgg taccaaattt ttccagaccg tttctgtaac ggcagacctg aaatatcccc
    11161 agaaggggta gaaccgtggg gcagtgcacc gacttcattc aactttatgg gtggggatct
    11221 gtggggggtt attgataaac tcgattatct ggaagatctg ggtattaatg ggatttattt
    11281 ttgtcctatt tttacctcag cttccaacca taaatatgac accatcgatc attttagtgt
    11341 tgatccacat ttaggtggga atattgcttt ccatactttg attgaagaag cgcataagcg
    11401 tggaataaaa atcatgttgg atgcagtatt caatcattta ggcgctgact ctccaatatg
    11461 gcttgacgtt gtacgcaatg gtgccaattc acgttacgcc gattggttct ggattcataa
    11521 atttcctgtt tatcccgata ctccaaaaag tgaatgggat tttaaaaact ttaactatga
    11581 aacctttggc aatgtaattg aaatgcccaa gctaaatact gagaatgaag agtgccgtga
    11641 atacttactt agtatagttc gctattggac gcaaaatttt gatattgatg gttggcgtct
    11701 ggatgttgcg aatgaagtgg atcatcattt ctggcgagat ttcagaaagg tgattaaaga
    11761 tatcaaacct gaatgttata ttttgggtga gatatggcat gagggcaccc cctggttgcg
    11821 tggtgatcag tttgattcat taatgaatta tcccttaaca tatgggatta ttgattattt
    11881 tgcgttacag gatacaacaa aacaagaatt tatgacatcc gttacacgtt cttatctttg
    11941 ttatcctaaa aatataactg aagttatgtt taatcttctg gatagtcatg acaccgcgcg
    12001 tattttaagc gtttgtttga atgataaacg aaaagttaaa ctcgcctatt tatttatgtt
    12061 aactcaagcg ggctctcctt gtatctacta cggtagcgaa ataggaattg atggttttaa
    12121 atcaatgacg cttgagaata atagaaaatg tatgatatgg gatgaaaaca aacaagatct
    12181 tgaattacgg caatttatac gttggttaat acgtctacgt aaaaaacatc cacaatggtg
    12241 tgtggcatca atacaatgga aagatgttga acatcctacg gtgatcgctt atcaacgcga
    12301 taacatcact ttcttcttaa ataatagtga agatactgcc aactttattt atgacggaag
    12361 aagtatggag atctccggtt tcagttatga aattgaaggt ttacctgctg ctgatcttta
    12421 tgatttttaa atttcaatta ggatgtttgt tatgataatt gatgcacaca ctcatattgg
    12481 tgactttgtg aaaatccgta tgccagaaga tgtttttctg gcatctctgg acaagtataa
    12541 tattgatttt gcattatgtt cttgtggatc tgcagtagaa gttgaccatg accagaaccc
    12601 gattccggat gaagatcaag ttacacagca tgataataat gaacgtatgc tcagacttgt
    12661 tcgtcaacat tcaaagcgaa ttggcgcgtt tatgtggatt aagcctcgtc tggaaagttg
    12721 tgatcaagat tttgaagata tgattgccag caatcgagat atcatttatg gcatcaaagt
    12781 tcacccatat cattcaaaaa tggctttcaa ttcagataaa gttcaggaat atatacgcct
    12841 tgctcaaaaa tatgatctcc cggtagttag ccatacttct aatgattatg aatccagccc
    12901 acagttggtt tatgagatgg cacttaaata tccaaatgtg aattttgtta tgtgtcatat
    12961 ggggctcgcg acagataatc aggaagcgat cgaattgatt gcaaaacttc ctaatctcta
    13021 tggcgatact gcgtgggtta gggcggatat ggtttaccag tcgataaaaa tctgtggtag
    13081 tgataagatt ttatttggta ccgataatcc tatta
//
LOCUS       DAZFKM010000138        13010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFKM010000138 DAZFKM010000000
VERSION     DAZFKM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            302..883
                     /gene="mdaB"
                     /locus_tag="ACHKAY_004138"
     CDS             302..883
                     /gene="mdaB"
                     /locus_tag="ACHKAY_004138"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO5702716.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            914..1228
                     /locus_tag="ACHKAY_004139"
     CDS             914..1228
                     /locus_tag="ACHKAY_004139"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO5702717.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1276..3168)
                     /gene="parE"
                     /locus_tag="ACHKAY_004140"
     CDS             complement(1276..3168)
                     /gene="parE"
                     /locus_tag="ACHKAY_004140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO5702718.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3197..3778)
                     /gene="yqiA"
                     /locus_tag="ACHKAY_004141"
     CDS             complement(3197..3778)
                     /gene="yqiA"
                     /locus_tag="ACHKAY_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO5702719.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3778..4605)
                     /gene="cpdA"
                     /locus_tag="ACHKAY_004142"
     CDS             complement(3778..4605)
                     /gene="cpdA"
                     /locus_tag="ACHKAY_004142"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO5702720.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4630..5052)
                     /locus_tag="ACHKAY_004143"
     CDS             complement(4630..5052)
                     /locus_tag="ACHKAY_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO5702721.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5053..5682)
                     /gene="nudF"
                     /locus_tag="ACHKAY_004144"
     CDS             complement(5053..5682)
                     /gene="nudF"
                     /locus_tag="ACHKAY_004144"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO5702722.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5887..7368
                     /gene="tolC"
                     /locus_tag="ACHKAY_004145"
     CDS             5887..7368
                     /gene="tolC"
                     /locus_tag="ACHKAY_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO5702723.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7516..8187
                     /locus_tag="ACHKAY_004146"
     CDS             7516..8187
                     /locus_tag="ACHKAY_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO5702724.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8193..9353
                     /locus_tag="ACHKAY_004147"
     CDS             8193..9353
                     /locus_tag="ACHKAY_004147"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5702725.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9391..10179)
                     /gene="ygiD"
                     /locus_tag="ACHKAY_004148"
     CDS             complement(9391..10179)
                     /gene="ygiD"
                     /locus_tag="ACHKAY_004148"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO5702726.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10322..11095
                     /gene="zupT"
                     /locus_tag="ACHKAY_004149"
     CDS             10322..11095
                     /gene="zupT"
                     /locus_tag="ACHKAY_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO5702727.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11153..11323)
                     /gene="yqiD"
                     /locus_tag="ACHKAY_004150"
     CDS             complement(11153..11323)
                     /gene="yqiD"
                     /locus_tag="ACHKAY_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO5702728.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11584..12237)
                     /gene="ribB"
                     /locus_tag="ACHKAY_004151"
     CDS             complement(11584..12237)
                     /gene="ribB"
                     /locus_tag="ACHKAY_004151"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO5702729.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12341..12489)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12611..12901
                     /gene="ubiK"
                     /locus_tag="ACHKAY_004152"
     CDS             12611..12901
                     /gene="ubiK"
                     /locus_tag="ACHKAY_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO5702730.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3297 a         3473 c         3171 g         3069 t
ORIGIN      
        1 aaaatccatt tataattatc aacatgacac gttgatattt tcatctttta cttattcaaa
       61 gccatatttt caatcccaaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta
      121 agaattatga ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg
      181 tgagtcagag caagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa
      241 tttcaccgta ccgctctgtg acgtactata gtcggcaaac gtctcacctt gaggttaaaa
      301 aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact
      361 gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg
      421 catcgttcgc gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc
      481 tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa
      541 atatattgat gatgtattca ccgaaggtca tggaacgctg tatgccagcg atggccgtac
      601 ccgcaaagat ccgtcaaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat
      661 gctttctctg acctggaacg caccaatgga agccttcacc gaaaaagatc aattcttcca
      721 cggcgttggc gtcgacggtg tgtatctgcc gttccataaa gcaaaccaat tccttggcat
      781 ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta
      841 tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta
      901 gaaggagtta accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca
      961 ccgtcaggcg gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg
     1021 ttgccacggc tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc
     1081 accggattct atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct
     1141 gcaaaccccg cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa
     1201 tatccgcatc ctgcaacctg ggatttaaac ctgccttgtt tgcccggcca tcctgaccgg
     1261 gcaatgttct ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt
     1321 gcggcgatct tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg
     1381 atcgtcttca tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt
     1441 ttcacgcaat tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt
     1501 gcctttcttg cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta
     1561 ataaacctct ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc
     1621 gtgtttcacc aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg
     1681 cagaccatca gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag
     1741 atcgtcgctg tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc
     1801 cagcacttcg tcggaagaaa cttcccaggt gttaaggatc ttacctttca gtggcatgat
     1861 cgcctgatat tcgcgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac
     1921 aaggaacagt tcggtacggt taagatcctg cgcggtacag tcagccagtt tgccaggcag
     1981 tgctgggccg ctggtcagct ttttgcgcac cactttttta gccgcacgca tacggcgctg
     2041 ggcgctggaa atcgccatct ccgccagcag ctccgccgcc tgaacgttct ggttcagcca
     2101 caggataaag gcatctttca ccacgcctga aacgaatgcc gcgcattgac gcgaagagag
     2161 acgctctttg gtctgcccgg caaactgcgg atcctgcatt ttcaccgaca gaacataggc
     2221 gcagcgatcc cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcggta
     2281 ttcacagaac tcacgcatcg cgtccaacag gccctgacgc agaccattaa catgggtacc
     2341 gccctgcatc gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg
     2401 cagccacagt agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg
     2461 tttttctggc agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc
     2521 ctgatagcac cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc
     2581 ggggcacagt actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg
     2641 gctgtcaaag aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc
     2701 gcaagtgccg acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata
     2761 aacctgacca tcgcggcgca cgttaacttc tacgcgcttc gacagggcgt taaccaccga
     2821 aatccccacg ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc
     2881 tgcatgcaga cggcaaagaa tcagttcaac cgccggtaca ccctcttccg ggtgaatatc
     2941 caccggcatc ccgcgcccat cgtcaataac ttctaacgac tggtcagcat gtaagattac
     3001 atccacgcgt tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg
     3061 ccccaaatgg ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc
     3121 aagcccggtg agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag
     3181 caattcgagt tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat
     3241 aatcttcgaa gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag
     3301 cgtagtacgc caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc
     3361 agatcaaatc cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc
     3421 gtgactctag cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg
     3481 tcagcagttc aaacgggcgc accgccgggt ttaccaccac tgcgggcagc ataaaacatt
     3541 gtgacaacca ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc
     3601 cgccatgttc caggacaatg gattccagca gctctgccgc gtcggaagga tacggcggca
     3661 actgcggaat gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc
     3721 tcgcttttgc agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca
     3781 gtagccttct gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt
     3841 ggtcagcgtg ccatcagcat gtaactcgag agtacgccag ccgggcgcga tggtgtccag
     3901 cgtaaagttg gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg
     3961 gcgaccgttc cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac
     4021 gtgcggaaac ttcgccagca cggtatccag ttcgcccgcg ttacgcaggc tgtgttgatc
     4081 aagccaacta caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc
     4141 tggcgcatcg gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg
     4201 cggcacgcca aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac
     4261 ccgcttcgcc ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc
     4321 gtggttgcca ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg
     4381 ccgataggcc gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa
     4441 ttcgtgctgg tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtatttac
     4501 ccctaacagg gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct
     4561 gactctggcc tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt
     4621 ttcacgacgc tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca
     4681 gtccgctaaa aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg
     4741 ataatcatac cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat
     4801 cgcgtcatga tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc
     4861 agtctgttct attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata
     4921 ttgtgcgttt gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa
     4981 acggcgcaat tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt
     5041 gtaacgcttc atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg
     5101 caaagcgatg accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg
     5161 ttcccggctt accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc
     5221 gcttgcggtc gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc
     5281 cgggcttgcc aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc
     5341 aatcgcttcg cgacgggcaa catcttccac actttcaccc tcttctatca tcccggcaac
     5401 catctccagt agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat
     5461 cagcacaact tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc
     5521 aaaaatttcc cgccgtacct catgactcat ttgcccattg aatagacgat gacgaaatct
     5581 ataaagatct aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc
     5641 gtttttgcca aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact
     5701 aatagtgaat taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt
     5761 aaattgaggc acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggattc
     5821 tgctagaatc cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga
     5881 atgcaaatga agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg
     5941 ttgagccagg ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa
     6001 ttgcgtaagt ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt
     6061 ccattactgc cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac
     6121 gcgaacggca tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt
     6181 gatatgtcga aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc
     6241 acatatcaga ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg
     6301 ttgaatgcta ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa
     6361 ttagatcaaa ccacccaacg ttttaacgtg ggcctggtag cgataaccga cgtgcagaac
     6421 gcccgcgcgc agtacgatac cgtgctggcg aacgaagtga ccgcacgcaa taaccttgat
     6481 aacgcggtag agcagctgcg ccagatcacc ggtaactact atccagaact ggcagcgctg
     6541 aatgtcgaaa actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc
     6601 gaaaaacgca acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa
     6661 attcgccagg cgcaggatgg tcacttaccg acgctggatt taacggcttc taccgggatt
     6721 tctgacacct cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgac
     6781 agcaatatgg gccagaacaa agtgggcctg agcttctcgc tgccgattta tcagggcgga
     6841 atggttaact cgcaggtgaa acaggcacag tacaactttg tcggtgccag cgagcaactg
     6901 gaaagcgcgc atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgca
     6961 tctatcagta gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ctcattagac
     7021 gcgatggaag cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcaacc
     7081 accacgctgt acaacgctaa gcaagagctg gcaaatgcgc gttataacta cctgattaat
     7141 cagctgaata ttaagtcagc cctgggtacg ttgaacgagc aggatctgct ggcactgaac
     7201 aatgcgctga gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa
     7261 cagaatgcta ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca
     7321 tccgcacgca ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg
     7381 ggcttcggcc ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt
     7441 cccccttctc gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg
     7501 tcccggaaga caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac
     7561 tggagcgcgc gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct
     7621 ggctgtgaaa agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct
     7681 gcaaacccag gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc
     7741 gaacgtactg cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt
     7801 cagtgccagc aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag
     7861 caatccagcg ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg
     7921 ggcggcggcg cgggatttgc acagcagccg ctgttctcct cgaaaaaccc agccagcccg
     7981 gcttacggta aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc
     8041 accatgaccg taccgaagac agccatggca ccaaaaccgg cgaccaccac taccgttacc
     8101 cgtggcggtt ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaactggc
     8161 acctcttctc gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc
     8221 cggactggcg tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc
     8281 cgtactggtg tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag
     8341 aagtcaccgc cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg
     8401 atgagctgat gaccaaattc cgcattccaa aacacacctg gagttttgtg cgccagtcat
     8461 ggctgacgca ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg
     8521 aacctaaact tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct
     8581 ttcagtggat ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc
     8641 agtttaacag tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg
     8701 gcttccagtt gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc
     8761 agtatttgca ggactgcgca acggaagctg aaattgctac tgagttcctc tacatcgatg
     8821 atatcgggtt aggtgaaaaa ggtcagttca cggatttaca ggatcaggta atttccaacc
     8881 tgttcaagct gtatccgtgg gaatttatgt tgcgtgagat gttttccacc aagctggagg
     8941 atgcaggcgt acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc
     9001 taccgctact gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg
     9061 aagatgatca tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg
     9121 caaacgtgtc gatcattgaa aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg
     9181 aagaagggat gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc
     9241 tgattggtag ctggctggtg aacgatcaac ccgccggaat tggcattcgt gaagaccgtg
     9301 cattgatcac ccaggatatg tctcggtttt atccacatat ttttgttgag taatcaatga
     9361 tgccggatgg cattcgccat ccggtaattg ttaacctatc tgcaccgaca gcatactcag
     9421 gctgcccatt tctataccat cgaccggaat ggtaattggc tcctgcccat cccacgcacc
     9481 taagacatac aacaacggca gatagtgctc cggcgttggg ttcgataacg cgccaccttc
     9541 atggtcgagg taattcacca gaggatgctg ctctaccgcc ccttgccacg tcagattcgc
     9601 tttcacatac tcattaaacg acatcgccca aggatatggt gaactatcac cgtgccactt
     9661 cactgtacgc aggttatgca ccacgttgcc actggcgacc aacattattc cttcatctcg
     9721 cagcgctgcc agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat
     9781 actcaactgc accatcggga tatcggcgtc aggatacatc ttaatcagca cgccccagga
     9841 accgtggtca aagccccagg cttctttatc cagcgtcacc gggaccggtg ctaacagctc
     9901 aaccagatgc tgtgccagca caggcgaacc cggagcagga taatgcgtat cgtacagcgc
     9961 ctgcgggaag ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt
    10021 tccacgggta aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat
    10081 ccccaacttc tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc
    10141 gtgacctaaa aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg
    10201 tgtcattttg atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg
    10261 atgatgatca tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc
    10321 gatgtcagta cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt
    10381 tctcggcgtt ctcgggcaaa aaccctcgaa ccgcttactg gcgttttcgc taggatttgc
    10441 ggcggggatc atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga
    10501 aggaatgtcg cctgtgttgg gttatggaat gtttatcttc ggtctgcttg gctattttgg
    10561 cctggaccgc atgttgccac atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc
    10621 gttgccaaaa tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa
    10681 cttcccggaa gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg
    10741 catcgcactg gccgtcgcgt tgcacaatat tcctgaaggt ctggcggttg caggcccggt
    10801 ttatgcagca acgggttcta aacgcacggc aattctgtgg gcggggattt ctggattggc
    10861 agaaattctt ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt cccccgtggt
    10921 catggcggca atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt
    10981 aatgccgctc gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg
    11041 aatgtcagtg atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaagca
    11101 acatcagaag ttggccagcg catggacaac ttctgctagc ttcactaaag ccttattcta
    11161 ccttttcggc tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg
    11221 cgtctctgtc ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca
    11281 caacaagagc aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat
    11341 ttaacaatac taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata
    11401 tggtcgcata ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat
    11461 attaatgaaa ttttctcata gaaagagaag cggaattaac atcttgcata gcagggcaaa
    11521 ataaaaaccg aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg
    11581 ttttcagctg gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac
    11641 gagcgccata ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc
    11701 atcgtcatta gtcaactcac acagcacacc agccggttta aagcctgcca gcgtcatcaa
    11761 atcaatagtc gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag
    11821 tgggaaaacg tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc
    11881 gcgaacggtc gtaattcgat cagcggcaga aacaccggta gtcacacctt cagccgcttc
    11941 aatggtcacg gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag
    12001 atcaagttgt ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg
    12061 aatggtcagc gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc
    12121 gttttcacgg tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag
    12181 tgcattttca acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt
    12241 aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat
    12301 atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg
    12361 actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa
    12421 gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg
    12481 agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata
    12541 attctggttt atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag
    12601 ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc
    12661 aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct
    12721 acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca
    12781 agtcctgtta cggactcgtg agaaactggc gctgcttgaa caaagaatga gcgaactgga
    12841 aaatcgcagt actgagatta aaaagcagcc agatccagag actctccccc caacgctgta
    12901 aacatttgtc tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgctttct
    12961 attaccttat cgcttaattc cattcagaat atcatcacaa aaatgaatga
//
LOCUS       DAZFKM010000139        12728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFKM010000139 DAZFKM010000000
VERSION     DAZFKM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..491
                     /locus_tag="ACHKAY_004153"
     CDS             <1..491
                     /locus_tag="ACHKAY_004153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702731.1"
                     /translation="GDKLMRIKRFFLILALLTPFSSMANVSKWSTGETHGVRSYAVSS
                     KDNYTLTFECDVGFNNTDPNQVGTRLLTLMKTEPGGESFDAKKEQITLKVGDDEYPIS
                     SIGSSVGDSYWYGFWSDTPDMEVKTFDAYVDGKKIATFTLRKAAELFNAAPEDGCLKR
                     AK"
     gene            550..1116
                     /locus_tag="ACHKAY_004154"
     CDS             550..1116
                     /locus_tag="ACHKAY_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702732.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRITDQASKSLACPVDFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGDVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            1127..1738
                     /locus_tag="ACHKAY_004155"
     CDS             1127..1738
                     /locus_tag="ACHKAY_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702733.1"
                     /translation="MGFNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKGRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            1753..2634
                     /locus_tag="ACHKAY_004156"
     CDS             1753..2634
                     /locus_tag="ACHKAY_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HFO5702734.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDKKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            2716..6081
                     /locus_tag="ACHKAY_004157"
     CDS             2716..6081
                     /locus_tag="ACHKAY_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO5702735.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVAKAPSVDPIVDAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAVAGP
                     LWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQVNT
                     QKRTVQELQDQTSEIKEGNDKILSALDQIAKNTGKKKGGLLSKLFSLLGKGAGGVASL
                     LMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAVGKG
                     ALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLDLGG
                     LVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTISTK
                     FDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIKNPG
                     KHIKEAASNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITPELK
                     APVNSQQGTSDSKAESDAKQTNIAARVINAALDMAKDSNKTVKETANQIINANAVETG
                     NKAAQTIDAALGQSATGKEEALSAYEIDKRRFNNGKDVSLPKLNAAGYQWISDNADYF
                     DELERKYGLEKGILSAVASAESSAGQRTGNPVDKNGNKLSSALGAFQITKGTREDLGL
                     SDADAMDTRKAADGAARYLSMLMNRYNGDQGRAIAAYHAGMGHVDKGRVVAGTGEYVT
                     RVRGYQQMLNNGAVYGSKVDHSAPAIHEKIPDNAVIDQSTGLAFTPGDSPFEKGGLVD
                     KIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGTATAAGNVYVDTPMPVEEAR
                     PVANNSSYFDHLGAQMGIDGLFDKLRNSPGMRKNNAPEPASTSQVTTAANDLQQPTGR
                     MQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQMTSLLARIEEHTKDSAKGQ
                     GTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            6081..6437
                     /locus_tag="ACHKAY_004158"
     CDS             6081..6437
                     /locus_tag="ACHKAY_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702736.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            6434..7867
                     /locus_tag="ACHKAY_004159"
     CDS             6434..7867
                     /locus_tag="ACHKAY_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HFO5702737.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVRNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            7867..8703
                     /locus_tag="ACHKAY_004160"
     CDS             7867..8703
                     /locus_tag="ACHKAY_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702738.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSDSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            8782..9216
                     /locus_tag="ACHKAY_004161"
     CDS             8782..9216
                     /locus_tag="ACHKAY_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5702739.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            9228..12077
                     /locus_tag="ACHKAY_004162"
     CDS             9228..12077
                     /locus_tag="ACHKAY_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5702740.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRTDIHLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDLTSVGRDILAKTSTQAV
                     IQYLGLRELGTSGEKIPLLSTANTWSARQSFNGGITGALTGNADTATKLKTAININGV
                     RFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSPYGNVLHLKGATAA
                     GEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVIRSADSYILVNFYTGVGSCPTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPGSGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNS
                     AGNHSHNIPVGHTGAGNGVSAGFNAALGTGTTSSAGGHAHNVYIGAHNHTIGIGAHAH
                     SVIIGPHGHTITVNATGNEENTVKNIAFNYIVRLA"
     gene            12077..12655
                     /locus_tag="ACHKAY_004163"
     CDS             12077..12655
                     /locus_tag="ACHKAY_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5702741.1"
                     /translation="MTFRMSEQSRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWNLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEE
                     ASLLTAWKTYRVLLNRVDTTVASDVEWPVAPQ"
BASE COUNT         3541 a         2818 c         3241 g         3128 t
ORIGIN      
        1 aaggagataa attaatgcgc atcaaacgat tttttctaat tcttgctttg cttactccat
       61 tttcatcaat ggcaaatgta agcaaatggt caaccggcga gactcatggt gttcgttctt
      121 atgctgtttc cagcaaagat aattatacgc ttacatttga gtgcgatgtt ggatttaata
      181 atacggatcc caatcaagta ggaacacgac tactcactct catgaaaaca gaacctggcg
      241 gtgagtcatt tgatgctaaa aaagaacaaa taacgctgaa agttggtgat gatgaatatc
      301 ctatcagttc tatcggttcc tctgtgggtg atagttactg gtatggtttt tggtcagata
      361 cccctgatat ggaagttaaa acattcgatg catacgtaga cggaaaaaaa atcgcaacat
      421 ttacgctacg taaggccgca gagcttttca acgcggcacc tgaagatggc tgcctgaagc
      481 gcgcaaaatg acctgtcaca aatgactact cgtagaatcg gttaacacac cagattctac
      541 gaggtttcaa tgacaccacg acaattactc gaagacgtca aatcccgctt cacacctttg
      601 attgcggatg aacctgcctt actggaatcc ctgctaagaa aagcattggg aacctaccag
      661 gatagggcgg ggcacatcaa gcggatacgc atcaccgatc aggccagtaa atcacttgct
      721 tgcccagttg attttcttgc gctcgtatcg gttacagatc acaccggcga tcttgtctac
      781 tccgatgttt acgatgggaa tatcgagctt gaagataccc atcgagcggt atacccactg
      841 aatgtgtcat atctggctaa tttgcgtgat atggatctgg ataatgggga tgtgccacct
      901 gaaatcattg ggttactttc tgactatctg gaagtgctaa tcgcgatacc taacactgat
      961 cgcctgcgaa gaatatctat cgcggggaaa ctcgatgcca gtaatttatc cgacgagaac
     1021 acgctgtatc agcgaaagct ggatctggaa gagaaaatga gcgcaacaag ggcaattatc
     1081 ccgggaattg ttcttttctc atccatgttg aagtgagagg gctgatatgg ggtttaatgt
     1141 tgcttcagta aagtcttatg tatcttcggc attaacgacg acattatttg gctccggcgt
     1201 tggtgagcgg gaagttggta agctgacgtc aatcatcatg aacaaaatgc tgttcgcgca
     1261 aggatggcag ttctctgtcg aagttgatgg tctggagggg gcagacttct ttgccaaaga
     1321 cattacctac cacgattaca gcatcgaata tgaaacgatt aaaataggcg gagggaatat
     1381 ccttcagcca acggagcgtt cgcctgggca gataacaatg atggtcaggg ataccgttga
     1441 tggcctcgtt ttggactggt ttaagacggc aaaaggtcgg gtgatcaatc cggacggtac
     1501 cgggaatata ccgtctaaat atttgctcaa tgtgcgtatt tatcggttgc tgtcctccgg
     1561 tttaaccaaa ctggaaaatg agatgacggt attcccggtc actaccggcg atgtcaccta
     1621 tgcgcgggat caggttacgg aatttaagtc attcccaatg accttcgcat tgcacagcac
     1681 gtttaaccaa tcctcaagtt ctttagcttc ccttctgggc tttagttttt ctctttgaat
     1741 taaggagcaa ggatgctttt acctcttttc ccgctaccat cgcggccaac tgaattgatc
     1801 cagttccgtc agccaaatat tgctgatgcg atgcgtttca actcgataac accggaggaa
     1861 caagaacaac agacaacggc gtatttaaaa gccttgctgg ctgaacccgc gaaatatgat
     1921 cccctgacat ggacggcgca ggaccggatt accgcgttat ggtggatatt tactggctcc
     1981 cgtgaaacac cggtcgagac attcacctac acctgtaaac attgcggtaa agagcattat
     2041 tacgattgcg atatgaatgc tctggctgaa gatatccagg tcctggaagt ggaaccgttc
     2101 attgacgata ttgaggtgtc tgtagagggc gtgccttatc aatggcgtat cgtgccgctt
     2161 gatggttggg caatggaaat gctggagatg cgccgtgcag cattgccacc tgaagacgac
     2221 gcggaattca aagaagcgat cgttgatttg cgtttttggg aattcgctta tcagtgtgag
     2281 ctttataacg atgttagcgg tactcgtgaa gatcaggctg agcgtcgtta tgaaacgatt
     2341 aaacggatgg ccattgatac tgaatttatg aagctggcgg cacacatccg actggctcat
     2401 gaaaagctcg aacatggttt accgtgctac atcgataaag gtgaaatgcg tcttcgtctc
     2461 ccgccgcata aatgcccaaa tcaggataaa aaggagtcca cagagggtgc atatacccgt
     2521 ctgtgggtgc cctttcgggc taccgacttc attccacagg tggggattga aaagctatca
     2581 gaccttagtg tccaacctgg ttttgtatgg gggtataccg attcaggacg ctgaaaggct
     2641 tactgaatcc tatgcgtttt tcctgttgga gaaactggaa gaaaaactta aaccgaaacg
     2701 gtaggcgata agatcatgga aagaaaaaac gccaacattg acgatgtgat aaggacagtt
     2761 gaaaccgcca gcgcaaaaga gctggaggag cttgctggta tccgggaagc tgttgaagat
     2821 ttgaaaggtg ggcgagttgc tactgttgat cctgtctctc gcagcgtgtc ggcattaaat
     2881 cgcacaatcg aaaattcccg gcctgacttt gtggccaaag cgccatccgt agaccctatt
     2941 gttgacgcaa tgaaacggct taatttaggg gacgtttctc gtgtagttca ggaggatgtt
     3001 gctctacagg aaccgcaggc caaatcaact acgcgaaagg gtaaaaaacg acgcaagaag
     3061 gctataacag aagatgtaaa ggcgcaacgg accgaagcag ccgaacacgc tcgcgaaatg
     3121 ttcggtcaaa aaggcggtgc gcaaaaaagc caaaaccaac gcgatgcgcg tggtcgtttt
     3181 attggaaagt cagggagtaa ggccgcagcg gaagatgccc gtgctgaaaa ggccaggcgc
     3241 aaagaggatg atgagcgtct aaatgctgaa tcaggtttat taaaaaaact gtcaaaagta
     3301 gctgaaggca taggtaaccc ttcagagact cgtgccgtcg atgcgttagg ttatgccgtt
     3361 gctggtccat tgtgggccgc agggaaggag cttggcggga tatcaaaaga agttggtgga
     3421 tcgcttaatg gtgccagaaa gtctattgcc gatgtgattc gtggcaatga cgataacagc
     3481 cgtagaaaag gtttttttag gcgtaaatcg caaaatagtg ccgatgtcgt tcaggttaac
     3541 acccaaaaac ggacggttca ggaacttcag gatcagacca gcgaaattaa agagggcaat
     3601 gacaagattc tcagcgccct tgatcagata gccaaaaaca ccgggaaaaa gaagggcggc
     3661 ttgctgtcca aattatttag cctgttaggg aagggggccg gtggcgtcgc gtcgttgtta
     3721 atggggcgtg gcatgctgaa aaaagctgga gcactcgctt ttggcgctct gggggcaaag
     3781 aaacttgtag gaatgctacg cggtggtggc aagaagactc tcgcccatga aggcggagat
     3841 ttggctgccc gggcagcagg taaacttgga ttaaaggcag ttggtaaagg ggcgttacgc
     3901 gcaattcccc tagtcggcac agtggctgga ggtatttatg atgcggtaac cggttggaat
     3961 gatacagaag cgcaacgtcg agcgtttggg cttaaatcag gacaagatcc atcattccag
     4021 caaaaagccg cttatacgtt agccaatgtt cttgatttgg gggggctggt atctggtatt
     4081 agcagtgcta ttggtgatgt tctcaaatca cttggatttg aggatatcgg caatatgttg
     4141 caatcatttt cgacggaaag tattgcccag gccattgata gtgggattac caacttagaa
     4201 acatatattt ctaaccttgg cgacaccatt tctaccaagt tcgatgatta cacagcaaag
     4261 attggtgatg ctgtttcagc atggtttagc gatacatcta ataagctgct tgaaaagctg
     4321 gatgccatca aagacttctt tactgtcgat aacctgaaac aggttttcag tgatgcaatt
     4381 gatagtgcaa ttgatttcat taagaaccca gggaaacaca ttaaagaggc agctagtaat
     4441 atttgggatg gggttaaaaa tttaccaggt aaagcattag atgcagcggt tgatgccgtt
     4501 aaaaataccc ctgcggcaat gattgtatca aaaataccca atccgatcgg cgaggctaat
     4561 gcgaaagaaa tcactccaga gttaaaagct ccggttaata gccagcaggg gacatctgat
     4621 tctaaagctg aatccgatgc caaacagact aatattgctg cccgcgtgat aaatgcggcc
     4681 ctggatatgg cgaaagatag caataaaaca gttaaagaaa ctgctaatca gattatcaat
     4741 gcaaatgccg tagaaacagg aaataaagca gcacaaacaa ttgatgctgc cttgggccag
     4801 tctgctacag gtaaggagga agcattaagt gcatatgaga tagataaacg tcgatttaac
     4861 aatggcaagg atgtttcttt gccaaaatta aatgctgccg gataccaatg gatttctgac
     4921 aatgccgatt attttgatga gcttgaacgt aagtatgggc ttgaaaaagg gattctgtca
     4981 gcagttgcat ccgcagagtc tagtgcaggc cagagaactg gaaatccagt agacaaaaac
     5041 gggaacaaac tttcatctgc ccttggggct tttcagatca ctaaaggtac aagggaggat
     5101 cttggactca gcgatgctga tgccatggat acacgaaaag cagctgatgg tgccgccaga
     5161 tacctaagta tgctgatgaa ccgttataac ggtgatcagg gtcgtgcaat agctgcctat
     5221 catgctggta tggggcatgt tgataagggg agagtagtcg ccggtaccgg cgaatatgtt
     5281 actcgtgtca gagggtatca gcagatgctc aataatggtg ccgtttatgg ctctaaggta
     5341 gatcatagcg caccagcaat tcacgaaaag atacctgata acgctgttat cgatcagtct
     5401 actggcctgg cgtttacccc tggtgatagc ccgtttgaga aaggcggtct ggtagacaaa
     5461 atcggcaatg ctgttggcgt taacgatctg gtcaacaaat tcatgaatgg ccgggggatg
     5521 cgtcgggaag tcgttcaggg aacgctagaa gaacgtgcac gagggaaggg gaccgcaaca
     5581 gcagctggca atgtgtatgt tgatactccg atgccagttg aagaggcgcg tccggtggcc
     5641 aacaactcaa gttactttga ccatctcggc gcacaaatgg ggattgatgg actattcgat
     5701 aaactccgca actcgccggg gatgcggaaa aataatgcgc ctgaaccagc ctccacgtcc
     5761 caggtgacga ctgccgccaa cgatttgcag caaccaaccg gtcgtatgca gatagacggg
     5821 caggttatta gtgaccttgg cggttccggt gccaagccga caatgcagtt ggctgataat
     5881 accgtttcac ttgatggtga aacgaagcgg ctgtttgcgc agatgacctc attgcttgcc
     5941 aggattgaag agcacactaa agactcggcg aaaggccagg gaactgtcgt aaaggtcagc
     6001 acgcctcagc cgggcgttat gcgcacggta ccactgtcaa ttgatgatcc gttgatgaat
     6061 gactacgcga gagttgattg atggccaaca ataacgaaat tgatcctttg ctgacgctgg
     6121 agttatccgg cgtaaaaacg tatgagtccc aggaggaggc ctggggcgct cgtttatatg
     6181 agtggctaaa cacttatcag ggtgaggtat acggagatcc gtcatggggc aatgttttac
     6241 cgcagtttaa acacgaaccg accaacttgt cgcatgttca aattgcggtt gaggcaatgc
     6301 tgttgcaaaa actgacggta gatttacctg acataccgat ttctggcttg tcagtagccg
     6361 agggagatgc ttttgataag ttgaaaatat ccattcgtat cagggatata actatcacac
     6421 aggacgtggt gctatgagta aaacaacacc gactaaagac agtattcgtg cagagtttga
     6481 agagcttgtc gagaaagatt cattctggtc gaagtttgtc ggctctcaat ttgtctcgat
     6541 gctgacattg tttattaccc agattgtcta caggtgcttt cagtatgccg atgcggcgct
     6601 ggctgaaggc tttatatcga ccgcgacgcg gcgttcctct atcctggcag cggcagaaac
     6661 gaatagttac gttggtacca agccaacacc gtcatcgggg atgattgaga tcaccgccac
     6721 aagtgaagat gccccagcgg taatccccaa aaacatgcct ttaatatctg acgaccagta
     6781 cccttacatg actatggatg tatgcaggtt ggttgacggc accggtacgg tagaagtggc
     6841 acagttggaa atccaggagg tgacatatac cgttacggca gccaaagaat ttctggaagt
     6901 cgtgttatca aaggctctca ctgctgtctg ctataagctg gaagtattcg tgacgaccga
     6961 tggtaagacc acgcagtggt cttccagcac tatgttccgg ttagccggta gtaaaagcca
     7021 ggtctacgtt gagttttata agccatccga gcagttgggg gttcgattcg gtgatgggct
     7081 aattgggcaa ataccgccag aaggctcgac cattacactt aaggtatggt gcaccaacgg
     7141 agatataacc ctggttgctg gccaaaatct gacgcctgtc gattctgcgg ctaatttagc
     7201 taatttgatt tcagttaaga caacgacacc cataaccgca ggtaccgatg ccgaaacaac
     7261 ggagatcaca cgtaatcgtg cacaatatta ccttgcctat gatgatcagg tcgtatgggg
     7321 cggggactat acgtattttc tggtgcgtaa catcccggga ctgtcctggg taaaggcatg
     7381 gggcgaaggc cagcaagaga aattagatgg tgcttataat gttcggaata tcaataagat
     7441 atttatttca ggatggcatc caaacaaaag ccagtcagag cttgaagaaa tgatcctggc
     7501 tgcctttaag aaggtgccga atgagttgaa caagaaattc tcgtataaag aggtcagaaa
     7561 actccccttt aagatcacca tcactgggcg gatatcggca agcctgacca ttgagaatgt
     7621 gaccgatgag ctgaagtcgg cactggaaac aaaatttggg cgcgactcaa ctttctttga
     7681 tccgaaccgc gtcgggaagt acatcctgat taagaaaaaa gacgtttggg cgtttatcga
     7741 aacgctgggt tatttccgcg acttttatct ggaatttgtc gagtggaatg agtccaacgg
     7801 cttttacgat ttcgtttatc tggatacaga aaactccacc tttaatattt cgtatgagga
     7861 ggagtgatgc agcgttcctg gtttaataac cggcttacat cagctaagca aaagtcattg
     7921 ctctataaat cattggctga tttggttcag tcaatgatgg acacctttgt tgacccatgg
     7981 ttggagcgaa ttaccaaccg gaagtctatt ttctccatga gcaaggagga tctggagacc
     8041 aggacaaatg aacttggcca gttctttact atcagaacct cggactcatc ttccgttccg
     8101 atgttgttac aacagcggct tgatgagatc cactttaagg gaactgaacg tcctataaac
     8161 cagacaattt accgcgaatt taacggtatt tctgttttat gggatccgat atatgcaccg
     8221 gtggaccttg agcgtcatcc ctatggcaca gttctaatac cagaaagcac attggagact
     8281 accggcggca cattcggcga gatgtttctg acttccagag ggatgatcag tattcccata
     8341 aacgacctgg cccggacaat gggtattact ggcacgatag atcagtccgc aattacagaa
     8401 gaaattctca gaaagtttaa tcagttcgta aagcctctac tgccactgca tatagtgttt
     8461 gatgggctta cgctctattt gtcggttgtt gtaaatgaac aggccgacat gatcactttg
     8521 aacgagattt ctgataccga aaaagcattc tgctggtttg aaacttcgga tacaacttcg
     8581 cttactggag ttacgtcgat tagcgccccg atcaccgcaa cgcctggtgg cactattgtg
     8641 aaagcgacac ctacgtttga tcgcacccgc gcagatgatt tgttgctgga tagcgacgcc
     8701 tgacaatcac cccgtccgca gggcggggtg acaagttact tctcttacaa tgaggcttca
     8761 caacagtgat tagggaaaat catgtctgac gtctcaacaa acctctataa gagtcagttg
     8821 ttggactatt actatcagcg gcgcgctgaa tcgtccatta acaaaggctc tcgattttta
     8881 atcagcaagg ccgttttcgg taccagttct ctggttacta agaaaggaga tggcacttat
     8941 gagattggag aactgccaaa ggctttcgat ctggcagaac tgaccagcca attttgtacc
     9001 atcaacctcg tgccaaccta ctcaggtggg ataattactg tccgaatgga ccttgatcaa
     9061 agccagttgc aggaagggaa aaactaccca ttcaacactc tggttgttct ggataacgag
     9121 aacaagccaa tcgccattat ttgtgtccag gaagactcgc tgtatgtggg caaaacatat
     9181 accgcagtta tggccataaa cacgactaca gcataaggat atgcttgatg aatgacgtta
     9241 cagttgttac atcggttact tacccatcac ccgagtcgtt ggctctggtt gctgatgtgc
     9301 aataccacga accatatctg tcagccgcgc taaaccgaaa attcaggggg attgttgacc
     9361 cgggatttta tgccggtttc ttacctaagc ctggcggtgg gatgaacctg ttaatcacct
     9421 cagtggatgg agataaaacc gcaggcgcgg cgtcggtgga tattggtgaa ttctaccagg
     9481 taactattca gcaacgtacg gatattcatc ttgcacttag tgcaggcaag aaatatgcaa
     9541 ttgtgctgaa gggaagatac ctccttggag aggataccta tcaggttaat accgcgtcac
     9601 atattcatgc ggctgaattt gttaccagaa cctataccga ttcatatcag ttaggagatg
     9661 gggagctgct tgtttgtacg gtgaatatcc ctgctggagt atcagccatt acccaggaga
     9721 tgattgatac atccgagcgt attaaccgca cgatcggcat tgatatttca gactctgtaa
     9781 ccagtagcag aagtgatgtt gctgcaagtt cgctggcagt taaaaaagcc tacgatctgg
     9841 cgaaaagcaa gtatacggcg caggatgcaa gcacaacgca aaagggatta gttcagctca
     9901 gtagcgaaac taacagcgat agcgaaacaa tggcggctac ccctaaagcc gttaagtctg
     9961 taaaagatct tgctgatacc aaagcgccaa tagaaagccc gagtctgaca ggaacgccaa
    10021 ccgcgccgac ggcggcgcaa ggtacaaaca gcacacagat cgcaaataca gcctttgtta
    10081 aggcagctat aactgcactt atcaacggtg cacctggcac actggataca cttaaagaaa
    10141 tagctgctgc gatcaataac gacccgaatt tcagcacaac tatcaacaat gctctggccc
    10201 ttaaagcgcc tttagcaagt cctgcattaa cgggaatacc tactgcgcct accgctgcac
    10261 agggtacgaa taacacgcag attgctacga ccgcttatgt aagagctgcc atatccgcat
    10321 tggttggttc atcaccagaa gctcttgata ccctgaatga gcttgccgca gcacttggca
    10381 atgacccgaa ctttgcgaca acaatgacaa atgcgctggc aggcaaacag cctctggatg
    10441 caactttaac cgctctcgct gcccttgcga ctggtgcaaa caaactgcct tatttcactg
    10501 gtaaggatac ggtagctcag actgatttaa cgtcagtcgg tcgcgatatt ctggctaaaa
    10561 caagcacaca ggccgttatc caataccttg gtttaagaga actcggtacc agcggtgaaa
    10621 agatccccct gttgagcacg gctaacacat ggagtgcgcg ccagagtttc aacggcggga
    10681 tcaccggggc gctgacaggg aacgccgaca ccgcgacgaa attaaaaaca gccataaaca
    10741 ttaatggcgt caggttcgat ggttcggctg acattaatat caatactctg gtatctcgcg
    10801 gtcgcgtaac ggccctagag gcgaatgcac agggaacatc cgggattcag ctgtatgagg
    10861 catacaacaa tggctaccct tccccctatg gcaatgtgct tcaccttaaa ggtgccaccg
    10921 ctgctggcga aggtgagtta ttcattggct ggagtggcac gagcggtgac catgcgcccg
    10981 tacatatccg ttcgcggcgt gatactgatt ctgccaactg gtctgaatgg gcgcaggtct
    11041 atacgtcaaa agattcaatt cccggcgtca atgccaaagg ggatcaggac acctctggta
    11101 atgcggctac agcgaccaag ttgcagacag catgtactat caatggcgtc tcgtttgatg
    11161 gttctactga tatcacttta accgctgcgc atgttgctgc ttttgccaga agagcaacgg
    11221 atacgtatgc cgatgcggat gggggcgttc cctggaatgc cgaatcaggc gcttacaatg
    11281 tcattcgctc tgccgacagc tatattctgg ttaacttcta taccggagtc ggaagttgcc
    11341 cgaccttgca gatgaaggcg cattacagaa atggtggtct gttctaccgt tcttcaagag
    11401 acggttatgg ttttgaggaa gactgggcag aagtttatac ctcgaaaaat cttccaccag
    11461 aaagctaccc agtcggcgca ccaatcccgt ggccatcaga taccgttccg tctggttatg
    11521 ccctgatgca ggggcagact tttgacaaat ctgcataccc gaaacttgca gcggcttatc
    11581 cgtcaggcgt tattcctgat atgcgtggct ggacgattaa gggcaagccc ggcagtggtc
    11641 gtgccgtatt gtctcaggaa caggacggca ttaaatcgca cacccacagc gccagcgcat
    11701 ccagtacgga tttggggacg aaaaccacat cgtcgtttga ttacggtact aaaacgacca
    11761 gttcatttga ttacggcaca aaaactacga atagcgctgg aaatcattca cacaatatac
    11821 ctgttggtca cactggcgcg gggaatggtg tatcagccgg ttttaacgct gcgttaggta
    11881 ctggtaccac gtcgagcgca ggcgggcatg ctcacaatgt atatatcggt gcccataacc
    11941 acactatcgg cattggtgct catgcccatt ctgtcattat tggtccccac ggacacacca
    12001 tcaccgttaa cgctacgggt aacgaagaaa acaccgtaaa aaacatcgca tttaactata
    12061 ttgtgaggct tgcataatga cattcagaat gagtgaacaa tcacggacca taaaaattta
    12121 taatctactg gctggaacca atgagtttat tggtgaaggt gatgcataca ttccacctca
    12181 tacaggtctg cctgcaaaca gtaccgatat tgccccgcca gatattccgg ctggcttcgt
    12241 ggccgttttc aacagtgatg aggcatcgtg gaatctcgtt gaagaccatc ggggaaaaac
    12301 cgtctatgac gtggcttccg gcgacgcgtt atttatttct gaacttggcc cattaccgga
    12361 aaatgtcacc tggttatccc cggaagggga gtttcagaag tggaacggca cagcctgggt
    12421 gaaagatgca gaagcagaaa aactgttccg gatccggaag gcggaagaaa caaaaaacag
    12481 cctgatgcag atggccagtg agcatattgc gccacttcag gatgccgtag atttggatat
    12541 tgcgacggag gaagaggcat cgttactgac tgcatggaag acatatcggg tattgttgaa
    12601 tcgtgttgat acaacagtag catcggatgt tgagtggcca gtcgccccgc aataaaagga
    12661 taaagccatc gatagaaata ttgatggctt catgtactct atttatacaa tacaacaccg
    12721 ctcttttt
//
LOCUS       DAZFKM010000140        12604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFKM010000140 DAZFKM010000000
VERSION     DAZFKM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..524)
                     /locus_tag="ACHKAY_004164"
     CDS             complement(39..524)
                     /locus_tag="ACHKAY_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5702742.1"
                     /translation="MTTVLHNSTTPSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(617..1435)
                     /locus_tag="ACHKAY_004165"
     CDS             complement(617..1435)
                     /locus_tag="ACHKAY_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5702743.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLLLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(1762..4608)
                     /locus_tag="ACHKAY_004166"
     CDS             complement(1762..4608)
                     /locus_tag="ACHKAY_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO5702744.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKCAGVAVALSLAA
                     VTSVPALAADTVVQAGETMSGGTLVNHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTAGNTAISSGGLQFVGAGGKATDTIINEGGGQSLKGLALNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGYALDTTLNGGNQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTIINQKGKLQVDAGGVAVDVTQHQGGALVTSTAATVLGSNRQGNFAVE
                     NGKAEGVVLESGGRLDVLENHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGNAPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(4975..5847)
                     /locus_tag="ACHKAY_004167"
     CDS             complement(4975..5847)
                     /locus_tag="ACHKAY_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5702745.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRNILPELDLVLWLIKADDRALSVDEYFWRHILHQGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            complement(5931..6848)
                     /locus_tag="ACHKAY_004168"
     CDS             complement(5931..6848)
                     /locus_tag="ACHKAY_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702746.1"
                     /translation="MVQKILSDNVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKAQAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQKNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKEKNIALENLASFPGSADIYHA
                     IHLSFDIWVTHPSASPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRARRLSTGEMLHDLIVEEYKRYRHSR"
     gene            complement(7680..7877)
                     /locus_tag="ACHKAY_004169"
     CDS             complement(7680..7877)
                     /locus_tag="ACHKAY_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702747.1"
                     /translation="MFFTARYTPGSQQTECAGAVIHQKWHAAVHTHPGDNMYVATAIP
                     ALWKRLTQPAGYSFRHPGMSA"
     gene            7946..8143
                     /locus_tag="ACHKAY_004170"
     CDS             7946..8143
                     /locus_tag="ACHKAY_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702748.1"
                     /translation="MAQYDDDEHALQVSMRGREHHHFRILSHLPEATGISCGYRQPLC
                     GMPGARTDPSDPSVLRSASDG"
     gene            complement(8049..8651)
                     /locus_tag="ACHKAY_004171"
     CDS             complement(8049..8651)
                     /locus_tag="ACHKAY_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702749.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFHNLT
                     PGWVSFNGEKPEIAIVPRSLHRLIYGPDKRATPPLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            complement(8738..8944)
                     /locus_tag="ACHKAY_004172"
     CDS             complement(8738..8944)
                     /locus_tag="ACHKAY_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5702750.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            9661..9873
                     /gene="hha"
                     /locus_tag="ACHKAY_004173"
     CDS             9661..9873
                     /gene="hha"
                     /locus_tag="ACHKAY_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO5702751.1"
                     /translation="MEVKTKEDWLYQLRRCSSRETLEKVISHTRYKLTPAELKAFNSA
                     VDHWLAELTMNKLYDRVPASVRKYVT"
     gene            10342..10911
                     /locus_tag="ACHKAY_004174"
     CDS             10342..10911
                     /locus_tag="ACHKAY_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5702752.1"
                     /translation="MTYEYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRCEGKRVHTTRLHYVWAREFGENKGKKHYHLILLVN
                     RDTWCGAGDYRDPGSLAGMIKQAWCSALGVDAGCYATLANFPSRPVVWLERDDDAGLR
                     DVLNRADYLAKEYTKVNGTGERNFGCSRN"
     gene            11172..11555
                     /locus_tag="ACHKAY_004175"
     CDS             11172..11555
                     /locus_tag="ACHKAY_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5702753.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSSLRYHPQY
                     DTERPWFEHTDEGLTEHGHECPYVRPERREILLIKRLQQWVPDALPVVRKAHRHCRQC
                     HHDYYGERYCTHCHTGRFSEEAVAG"
     gene            11552..11965
                     /locus_tag="ACHKAY_004176"
     CDS             11552..11965
                     /locus_tag="ACHKAY_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5702754.1"
                     /translation="MNGLKTGLGITPGEHIISAASALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALTAQQKAACMVLNPEKSHPYIEDMAMFLSPLPTVLEWHCVMCERLF
                     HGKKFCEACGTGIYCRATCTKYVYRYQPDLLRDPE"
     gene            11971..12153
                     /locus_tag="ACHKAY_004177"
     CDS             11971..12153
                     /locus_tag="ACHKAY_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702755.1"
                     /translation="MRHNYLIMPVMPLPDPDVVRRFVPRTVVHWLCLLMILFMRHSDR
                     GDIDKHHHLRANSYTE"
     gene            12274..>12604
                     /locus_tag="ACHKAY_004178"
     CDS             12274..>12604
                     /locus_tag="ACHKAY_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5702756.1"
                     /translation="MRTQIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDT"
BASE COUNT         3011 a         3220 c         3308 g         3065 t
ORIGIN      
        1 ggcagaaagg aaatctgttg cattgtgctt ctctccattc agtcgatgat gcgcataatg
       61 gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg gaccgtcatg
      121 gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc ggcaataccg
      181 gcagcttccg ggctcatttc agcgcggtta ccgttcatgg cattgaacag tacccatgtt
      241 tcgtcatcat cttcatccgg ttcaggtgcc ataaatgccc cgccgttgtt cagggtatac
      301 agattccaga taccaccgca gtagtcttca cacagacggt ccatccagcc gaagacacgg
      361 ggctccaggg tcacccactg tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc
      421 tgttcatcag ggactatggt ggcaaccagt tgaggctggt cattccctga tgcagcggtt
      481 acggaaacag aaggagtggt ggaattatgc aagacggttg tcatgagatt attccttata
      541 aaaagtaaat gaatggaaga aaccccgggg ggaagggaca gacgtgagtc agaactgcgc
      601 tttcagggaa acggcatcag cgcaggctct ccagcagcgt ttcagccatc acccacaatg
      661 cgcggttgag cttaatgtcg gtgtcgatgc tgtgaatggc acgggtatgg atacgttttc
      721 ctctggcact gcgaccggaa attccgcctt tcagcatatt ttcctggatg gtctgataag
      781 cactccacag gtccttaccg taatcctccc ggcgtcgtgg cgtcagaatg tcggcagtgg
      841 tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc
      901 gtgccggtgg cggtaagagc agcgactgca tggcatcacg cttctcctct atccggtcaa
      961 acacgcccac cacctcgtaa gccccttcaa tgactctgtc caccacattt ccccggtgcg
     1021 gaacacgcac ttcccccaga gactgaccac agacgcagcc gttctggcag acaaatctga
     1081 agtaacccgg cagcatctgg tagctggagg taccgtcatg ggagttgagc agaataattt
     1141 cagggacatg ttgtccgttt atctccccgt cacggcgcag gcgcagcatg tgttttgtgt
     1201 atccccggcg gcccgggtca cgtacgcggg tctggcaggc aaagaagggc tgaaaacctt
     1261 cccgctgcag gctttccagc acggtgatgg tggggatcca ggtatagttc tcgcttctgg
     1321 atgtgtgttt ctcttccccg aatacgctgg ggacgacctg catcagttct tcacgagtta
     1381 aggggcggtc acggcgtatc tggttcgcat aaccaaaacg actggctaat cgcatgattt
     1441 actccttatg aatgagttag cggtataaat gaaaaagccg cgtctcccgg agaagacgcg
     1501 gcctgatggg ggaaatgaat gacgtttatt gtctgagaag cccttcactg gcgagctgcg
     1561 tatttttatg gaaggagagc acccaataac cccatcggga tcgggtaata accggccaaa
     1621 ttgtgaacga ttcagatgcc atttggcagc aaaagagcct tcggcaataa acgtgatgta
     1681 caacccggca tatctgttgc tccctgaaag taaaaggccc cggttattat gaccggggcc
     1741 tgaaggggag tgacctgatt atcagaaagt cacattcagc gtggcctgac cgttataacc
     1801 ttcagcgctg ctgccgctga cactgtgggc ataaccggcc tgaacgccca gggtgatatt
     1861 ttcccggaca cgggcttcca gtccggcctg caggtccagt gacgtgccat tccgggacgg
     1921 tgagaacgtc atgttactgc cggctgcggc tgtacccatg ctcatgtctc cccgggagct
     1981 gaaggtgcgg ataacagaag gctgtaccca ccagttcacc ggcagttcac tcacgctgtg
     2041 ttttgcactg tcgcgcaggg tgtcacggga tgaggtgcct ttaccaaagt tcatatcgtt
     2101 gtggctgccc agacggaaac cggcacgcac atgttgtgca ctgccatgcc cgaacttcac
     2161 ataaccggcg ttgtccttac cgtcatccag ggaaagtccc tgccaggtat actgcagttg
     2221 tggctccagc atcaggttgt cagtgatact gaagggcaga ccggtttcca gtgagcccag
     2281 ccagccccag ccccgggcgc ggaagtcgtt attgtccgat gacgctttca tgctgtggcg
     2341 ggttccctgt gccacaatgt cagcccacag gccggaggac gtgtgtgtca gattcaggta
     2401 tccgcccaga ctgccggcat catcccggac cgtgccggcg cgggaaccgt catcatcctt
     2461 aacatcaacg gaagaatggc ctgcagcacc atacacccct gtcgtcagag acataccggc
     2521 aacctctgtt ctgagcaggt caccctccag acggacgaag ccatagctgc cgctgctttc
     2581 cggcgtggcc ccacgggcaa taccaccgtt gttatcgtgc ccgagatgac cgccctgaat
     2641 gctgagacgg acgctgttat tttcaccgct tacaccggtc tgatggctgc gggagcctgc
     2701 cagaatccgg tcatagtcca ttgcctgtgt cagcatggag gcatacaggg ggacttcagc
     2761 acgataagca ttttcactgc gcaggtacca gtcttcatcg ctgtcacggt tcagggtgta
     2821 gttaaaggcg ccggcctgaa gcgggcgact cagggcaaac gcaccttctt ctgtggtggc
     2881 gccattctgt gcatccacaa cccggatacc ctgtccggag gttgccaccc cgaggttact
     2941 gtttccgaca tttgtaaacg caagccaggt tttgccggtt gcctgaccac cattaatcac
     3001 cagctggtca gaggtattgc tgccatcaag gcgaacacgc atattgatgg tgccaccctg
     3061 accggtgagg ttactggttg tcagtttatg gaacgttacc ggggcgttgc cttttgcaac
     3121 agcacggctc agtacggcgt ccggtgttgt ctgattatca aagtaaatct cccctgcgtt
     3181 aacaatatcg ccggtactga ccacatcacc attcagtacc atgctggcgc ctttactgag
     3241 ctgtgttctg ccactcagac ttcccccggc agccagcgtc agtgttccac gatatccgac
     3301 agtggtgtta ccggcctgtc ccccggcatt aaccgtaaag ctgccgccat tttccagtag
     3361 caggccgctg gcctgaccgg atatgccatc aatactgaac ttaccgctgg cattggtccc
     3421 ctcaacagtg gcaccactgt ctgcaatcag ggcaccaccg gatgtcatgg tgacacctgt
     3481 cgccttacca ccggcagaca ctgccagggt accgccgtca tccaccaggg tattctgtgc
     3541 tgaatggttc tccagtacat ccagacgacc accggactcc agaacaacac cttcagcctt
     3601 accgttttcc accgcgaaat tgccctgacg gttgctgcca agaaccgttg ctgcagtact
     3661 ggtaaccagt gcccctccct gatgctgggt aacatcaaca gcaaccccac cggcatcaac
     3721 ctgcagtttg cctttctggt taataatggt attcccggcc acgcccccgt ttttgacaat
     3781 ctgccagccg tcactgttca caacagtgtc agacgctgta ccgccgttgt gcacatactg
     3841 gttaccgccg ttcagtgtgg tatccagcgc gtagccatgt acagtctggt ctccgccggc
     3901 ataaaccaca gtggtatttg ctgttccttc agcagccaca atctgacgac catttttatt
     3961 gatggtggta cgtacggcat ttccgcgaac aaactgcccg gtatcaccat tttctgcatc
     4021 cggtccccct tccgcgccgg tattcacaac ggtgtctgtt gccactgcgc cgggtttgac
     4081 gacctgccag cccttatcat taatgacggt tcctgtggct atcgcaccct catgcatcca
     4141 ctgctcgcca ccattcagcg tggtgttcag tgccagtccc ttcagacttt gtccgcctcc
     4201 ttcattgatt attgtgtctg tagcctttcc cccggcacct acaaactgga gaccgccgct
     4261 gctgatggcg gtattaccgg ctgtgccgcc atcctgtacc cattgcccgc cggtattggc
     4321 ctcgttatcc ggtccatact ccagcccggt actgatggtc attccgttgg tcgtaccgaa
     4381 gacaatctgg ttgtcatgat ttaccagtgt cccgccgctc atggtttctc ccgcctgtac
     4441 aaccgtgtca gcagctagtg ccgggactga cgtgacagca gcaagagaca gagcaaccgc
     4501 cacaccggcg cattttcccc gtgagcgggc cagttcggag gccaccacca gggtgcccgt
     4561 aatgtgattc cataccagcc tgtagctggt gttcagatgt cgtttcatca gcttttcctt
     4621 acagagggtg aataaaaaag ccggtaccca cagatgtggg taccggcagg tacaaaacag
     4681 catggtcaga taaagtggtt tttcccggac ataaatgttt cccctctctc atccgccaga
     4741 gaagcatcag tccgttgtgt gcaattccgt tctgcatgat gaagagattc agctgccttt
     4801 tcgcaggcca ttaaccacta taaacgatcg atagtaacga tcgatacctg ttttatcgat
     4861 cgttttattc tattaacagc agggaagtca cgcaagagaa agaggtgggt atgagggggg
     4921 catgttttca gttggttaac gcaatgtaat caggcatttc ccctggcaga gtgttcagaa
     4981 gaaaatccag ttccatacag cgcgggcaac agagaccacc gagtcacgga cggcacgcag
     5041 aaccgtgcgt gcaacagagg caatacagac gctctccgcc gtgtcaaata tccggtccac
     5101 cgcaccggta aactgttcac gggcctgaga ccggacagat tccgtgcgca gctcgtcctg
     5161 caggcgggtc atcagggggc tggcggcatg gtcgggaagc gctgtcatga gcgcactgac
     5221 cagcgtatcc agttcccagc cggtgcgggc cgatacggca acaaccggat gtacgggccg
     5281 gaacagccgg aataccgcct ccgttttttc gcgaatgttc tgtgcctgtg cgggagaagg
     5341 ctgaataccg gccatatccc attcatggca gggctccgtt ttgtcggcct gcgtcaccac
     5401 aaacagcacc tgctgatgtc cctggtgcag gatgtgtcgc cagaaatact catccacaga
     5461 cagggcccgg tcatcggctt taatcagcca cagtaccagg tccagttcag gcagaatgtt
     5521 acggtacagg gcttcatact ctgcatccct gtcccggctc tcgcccaccc cgggcaggtc
     5581 agtgataacc atgctgtgac cgtggccact cagacggaag cgctgcactt cccgggtgcc
     5641 ggcgtgaaca tcactgaccg gggtgacctc cccctgaaac agtgcattac agagtgagga
     5701 tttaccggcc ccgcttttac ccataatgcc aatcacgggt tcgtgacggg tgagtttgcg
     5761 cagatgttcc aggatgtgac gggaaagcga gtaaggcaga gaggagaggg gtttttcaat
     5821 tgcctcaatg gcatcagacg gattcataca tgtattcccg gtgaaaaaag acaaaacccc
     5881 gcgcaccgga aaggaggcgg gggattcacg gagataatat atgtctgaaa ttatctggaa
     5941 tgtcggtacc ttttatactc ttcaacaatc aggtcatgca gcatttcgcc agtactgagt
     6001 cttctggccc tcgataattc tttgagctgc gccctggttt ctgcgtcaag aaaaaactgt
     6061 acattgactt tatcttcctg catttttttg tacttgcgct gtgccttggc cttattgaaa
     6121 ctgttcctga aattttttat atcgtcaggt gaggcagatg gatgggtaac ccatatgtca
     6181 aatgaaaggt gaattgcgtg gtaaatatca gcagaaccag gaaaactggc caggttttcc
     6241 agtgcaatat ttttttcctt taaataattc catgtccata tgcattcatg ttcgttctcc
     6301 gggaggaggt aactgaaagg gctctttgat ttaaatgccc gcatccatct gtctttggtc
     6361 tggattatcc actcatcccg caaattctta tcagaaagaa tatcgagaac tctgagtatt
     6421 tgtctgaact gtgtcgatga gtccgacggg aaggcattcc cttctaaata ctcttcttta
     6481 agatacagtt tattcttttg aagcaagtct gcgcttattt ttgccctgtt tttctggata
     6541 aactgcatca acagatacca ggaaaataag catgcgcgtt cactgcccgc gatccaggat
     6601 attttttcca gggggaccag gaaggcctgg gccttcagac gcataccaga aattatttgt
     6661 tcaagttcaa aatctgaaag tgtgatgttc agattttctc tgtttttctt tatgtaagcg
     6721 ataaaacgtt ccgggggatc ataaacatta aggggcagta cggagatatt acgctctcca
     6781 agataataag agtaatacgc atttgttctt tcattcataa cattatccga caaaattttc
     6841 tgaaccatcc tctttctctg gctgcttaaa aattaaacat aataaggtga taataacatg
     6901 ataaaaagaa gtttataatg gtatattaag atggtcaata aaaaggcaat ctgcgcgctg
     6961 tatagtcatt ctgacaggaa atgcaggagg aagggaaggt aaccggcttc gttatgctga
     7021 caggaatacc tgttatcatg gaagtgtatt catgtggccg acgtagctta agaggatgga
     7081 agagtggaga ctgcagacag ggagtattga aaggtaaaca tgtttcgccc gccagctgca
     7141 ccggaggcca gcatcacttt ttttacgttg taacccggta tgcattccgg ctttgatgat
     7201 tacagtcaga gggcagcaga ctgacgttat ttaaccggac agcaaaatta tggacactga
     7261 aatatcgtct gttaatccct cgtttgtcgg tgtggtattc cctgtattac cggcgtcacc
     7321 gataaagaga gttataaacg ccagaaaggt acctccttta tgccggggaa cacttacgga
     7381 aaatgaagtc tgaaaaggag cacaatgaaa agaggcgtgt cccgtaaata taacgaatca
     7441 tcgcaggata tacattatca ggtgatacca gagggcatta aactgtcgcc acggataaga
     7501 atatacaaca tttctgataa caaaacatca taacgtgttt cagatatgtt ccggcatcag
     7561 tctgacggat attatttccc ggtaaaacaa accggctact gtaacggtta actgtatctg
     7621 cataaattat ttatgatgaa agaacagtat tacggattca aatatcatcc tgtttgcatt
     7681 caggcggaca ttcccggatg ccggaaagaa tatcctgcag gctgagtgag tctcttccac
     7741 agtgccggga tcgctgtcgc cacgtacata ttgtcaccag gatgggtatg taccgcagca
     7801 tgccatttct ggtgaataac cgcacctgca cattcagtct gctgagaacc tggcgtgtat
     7861 cgggctgtaa agaacatccc tgtccgtggc tcctctggtg gtgttgcctg ctcagcaatg
     7921 acaggatgcc cttaagacgc ccgtcatggc tcagtatgat gacgacgaac acgctctgca
     7981 ggtgagcatg cggggccggg agcatcatca tttccgtatt ttgtcacacc ttccggaagc
     8041 aaccggcatc agttgcggct atcgtcaacc gttgtgcggt atgccaggag cacgtactga
     8101 tccatcagat ccgtcagtgc tgcgctctgc ctctgacggt tgatgatcca ttcctccagc
     8161 tctttttctg atttccgttt caggttctct ttccagtctt caaggctgtt tccggtcatt
     8221 aaatcaagcc agcaaagcca gaccagtttg aggcgcagtt ccgtgccacc aaactgacgg
     8281 aagaggctga tcagtttatt tgccagcgaa cgctgcctca ggcgttcaca ttcagacagg
     8341 agaatgcctt ccagcatcgg atgatgaacc agcagatgtt cactggtgca taagttcact
     8401 accagatcat catccagggg cggcgtggcc cgtttgtcag ggccataaat cagccggtgc
     8461 agactccggg ggacgatggc aatctccggt ttttcaccat tgaatgacac ccacccgggc
     8521 gtcagattat ggaagtagtg gatattcgga tgacacacgc cccaggatac ggcgtgccgg
     8581 gtgaagccgg tcagccattt ctggtagctt tcgtggagca gctcgtaacg ggtctgatac
     8641 tgtggtttca ttttcatatt cctcacgggg gataacaggc agcagaatgc cgccggcagg
     8701 ccggagactg cgtctgctgc agatgaacgg ttaagaatta cggacgggat tgtgcaatac
     8761 gcgcctgcag ccaggcttcc acttcgcttt tcagccatcg ggagctgcgg cccattttga
     8821 tgggcgcagg aaagtcacca tccttgatga gtttgtaaaa ccacttatcg gttaagccgg
     8881 tcagttgagt gataaatgcc atgtcgacca tctggtcatc catcagcgaa actggggtga
     8941 tcatgaaaat gttcctccgg atgttgttaa tctcaaccgg cgttggtgat gaaaacaacg
     9001 caccaccttc accggcataa caaccgggga gaaacgtaaa aaatgagtag gggaatattc
     9061 ctgccgggag ataaacaggg ggcatgatgc tgagataccc ggtggctacc gcgaaacgat
     9121 gcttcccgcg cgtacgcacg acagagaagg gatgcttttc gggaggatga ggggaagaac
     9181 cgtcgttctg ataattgcag agttatttac ggggcagtta cggtggggca gcccgtgtcc
     9241 ggtgggcagg cgacaggtat caccgatggg ataagactgt ctgattggta ttaaatatat
     9301 aagagagaga tcaaacacat gaaacacatg agatcaaata catgagttca tatatgtgaa
     9361 ataaacactg tgagtgtgat cagtgggcat gatgagaacg cagtagcaac agattgcgtt
     9421 gtacccacag ggctatccct gatagctggc gtataacctg cgtatataaa acgtacctgc
     9481 aacacacgtg aacagagtgt atttgagcgg taagcgtatc cgacctggtg agtgtcagtc
     9541 tgagataccg gttaagtggt ggcacgggag taatgagttt ggggagggta ccagaatgcc
     9601 agtctggtgc agtatgctga cagtatcacg ttaattgcag cataaagtga tgagaggcta
     9661 atggaagtta aaaccaaaga agactggctg tatcagctcc gtcgttgttc atcccgggag
     9721 acactggaaa aagtgatttc ccacacgcgt tataaactta cccctgcgga gctgaaagct
     9781 ttcaactctg cggtcgatca ctggctggca gaactaacca tgaacaaact ttacgatcgt
     9841 gttcccgctt cagtccggaa atatgtcacc tgaacgttcg gtaatcacag cggaattgca
     9901 ccaggtgtta tctgctctgt gtgattcttt ttgggttttc tcaggttgtt aaaagctgaa
     9961 tatgttcctg ttgtcggaaa ccaggataat accgggaacg ttaagcagta tagcgacaac
    10021 aaagaattcg aaatcaaaag ggaatggtgt ccggaggcgt gggcggtaat gggcagcatt
    10081 ccgtattatg agtaatcacg ttatcacaat ccctgcacac cgttttgctt atcaccatca
    10141 cgtgctcctc acagtcttat ccgtaaccgg ctttttgcat taaaaaaaca acactgaaaa
    10201 ttcagccgtc agtccggaga cgacagtcgg gttatcacag tgtgcctgag acattgcccg
    10261 gttggagatg acttcaggga ctctgcacat tttttacagc gtggttattc cgtatgaacg
    10321 aaaccatacg gagatttaac catgacatac gagtacaacc ccttctggca acaacgtatc
    10381 cgtgagacgg tactgcgcgc actggatgtt catccccgtc tgacggcatt gcgggttgac
    10441 ctgcgtctcc cggatgtacc ggcagcaact gacgcagcag tgatatcccg cttcatcaat
    10501 gccctgaaag cccgaatcga cgcttaccag aagcgtaagc gctgtgaagg taaacgcgta
    10561 cataccacaa ggctgcatta cgtctgggcc cgggagttcg gggagaacaa aggtaaaaaa
    10621 cactatcacc tgatactgct ggtcaaccgt gatacctggt gtggtgcggg cgattaccgc
    10681 gatccggggt cactggccgg gatgattaaa caggcgtggt gcagtgccct gggggtggat
    10741 gccggatgct atgccacact ggcgaatttt ccgtccaggc cggtggtgtg gctggaacgc
    10801 gatgatgatg ctggtttacg ggatgtgctg aaccgtgctg actatctcgc gaaagagtat
    10861 accaaagtca acggtaccgg tgagcgtaat tttggctgta gccggaattg agtacgactg
    10921 gcgcaccagt atccagtaaa accaaaagaa tggactgttg tctggctgca acgatgtacc
    10981 ttttctccta ctggttgttt tttacgccag agcgccattg tgtgacaacc acagtgctgt
    11041 aacaacaacc aacctgcttt acccaacccc ggatccagat tttaactgaa ccgccattca
    11101 cactctgacc acgcagcccg taccgggacg gctgacgcct gcgtgtcgtc tggcggttga
    11161 ggagtatgat tatgtacgca aaatccttta ttgcatttga tggaaacgga cgtctgaccg
    11221 gtgcccgtac cgcacagact gcgccatatg atcgttatac ctgtcatctt tgtggcagtt
    11281 cgctcagata ccacccgcaa tacgacactg aacgtccctg gtttgaacac actgacgaag
    11341 ggctgactga gcacggtcat gagtgccctt atgtcagacc ggagcgcaga gaaatactgt
    11401 tgattaaacg cttacagcaa tgggtaccgg atgccttacc cgtggtgcgt aaagcccacc
    11461 ggcactgcag acaatgtcac cacgattatt atggggaacg ctattgtaca cactgtcata
    11521 cagggcgttt cagtgaggag gcagtggcag gatgaacggt ctgaaaacgg ggctggggat
    11581 cacaccgggt gagcacatta tcagtgcggc ctcagccctt agcagaaata tcaggcattg
    11641 tttctgcctt tcctgtcgtg gtcggttgat tttgcagaca gatgctcagg gggcgtggtt
    11701 tgagcacgat ttacatgccc tgactgcgca acagaaggcg gcttgcatgg tgctgaatcc
    11761 ggaaaagtcc cacccttaca ttgaagatat ggcgatgttt ttatcgccat taccaaccgt
    11821 gctggagtgg cattgtgtga tgtgcgagag gctttttcat gggaagaagt tctgtgaggc
    11881 ctgtggtacg gggatttatt gcagagcaac atgtacgaag tatgtttaca gataccagcc
    11941 agatttgctc cgggacccgg agtgagggag atgcgacata attacttgat catgccagtc
    12001 atgcccctgc cggatccgga tgtggtccgg cgctttgttc cgcggacggt ggttcactgg
    12061 ctgtgtctgc tgatgatcct ctttatgcga cattcagaca ggggagacat cgataaacat
    12121 catcatctgc gggcaaatag ttacacagaa tgatcattgc taccggttat ggcaaaaatt
    12181 ggtgatgatc aggtgaacca tcggtgcttc cgttgattct tatggactga taaagttttg
    12241 tcagctgaat ctttatacgg aatgctcttc agtatgcgta cacaaattga ctatctggcg
    12301 gataaatact gttttacaga acggaacgaa tccccccgtc ttcgtcggca gtggcaggat
    12361 gttctggagg agtgccggca gacagaggcc gggccagaag aacggctgcg tattgcactg
    12421 ctgaatgtgg attacgtcac cagttttgag ctgccttttc gcttgctgct tacccggaca
    12481 ccacaactga ttgccgcgct gcgtgaagaa tggggcatca gccagaaaaa tgtggtgttt
    12541 aacgataaac ggtttggttg cgtgtacagc ctgaaggcca gcctttctgg tgtgccggat
    12601 acat
//
LOCUS       DAZFKM010000141        12598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFKM010000141 DAZFKM010000000
VERSION     DAZFKM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            221..1051
                     /gene="yjiC"
                     /locus_tag="ACHKAY_004179"
     CDS             221..1051
                     /gene="yjiC"
                     /locus_tag="ACHKAY_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5702757.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1192..1965)
                     /gene="uxuR"
                     /locus_tag="ACHKAY_004180"
     CDS             complement(1192..1965)
                     /gene="uxuR"
                     /locus_tag="ACHKAY_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO5702758.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2180..3640)
                     /gene="uxuB"
                     /locus_tag="ACHKAY_004181"
     CDS             complement(2180..3640)
                     /gene="uxuB"
                     /locus_tag="ACHKAY_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO5702759.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3721..4905)
                     /gene="uxuA"
                     /locus_tag="ACHKAY_004182"
     CDS             complement(3721..4905)
                     /gene="uxuA"
                     /locus_tag="ACHKAY_004182"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO5702760.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5357..5449
                     /locus_tag="ACHKAY_004183"
                     /pseudo
     CDS             5357..5449
                     /locus_tag="ACHKAY_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5692..6594)
                     /gene="fimH"
                     /locus_tag="ACHKAY_004184"
     CDS             complement(5692..6594)
                     /gene="fimH"
                     /locus_tag="ACHKAY_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5702761.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6614..7117)
                     /gene="fimG"
                     /locus_tag="ACHKAY_004185"
     CDS             complement(6614..7117)
                     /gene="fimG"
                     /locus_tag="ACHKAY_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO5702762.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7130..7660)
                     /gene="fimF"
                     /locus_tag="ACHKAY_004186"
     CDS             complement(7130..7660)
                     /gene="fimF"
                     /locus_tag="ACHKAY_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO5702763.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7670..10306)
                     /gene="fimD"
                     /locus_tag="ACHKAY_004187"
     CDS             complement(7670..10306)
                     /gene="fimD"
                     /locus_tag="ACHKAY_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO5702764.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(10373..11098)
                     /gene="fimC"
                     /locus_tag="ACHKAY_004188"
     CDS             complement(10373..11098)
                     /gene="fimC"
                     /locus_tag="ACHKAY_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5702765.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11135..11674)
                     /gene="fimI"
                     /locus_tag="ACHKAY_004189"
     CDS             complement(11135..11674)
                     /gene="fimI"
                     /locus_tag="ACHKAY_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5702766.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11739..12287)
                     /gene="fimA"
                     /locus_tag="ACHKAY_004190"
     CDS             complement(11739..12287)
                     /gene="fimA"
                     /locus_tag="ACHKAY_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5702767.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3140 a         3247 c         2974 g         3237 t
ORIGIN      
        1 ttccaattag taataagata tgcttttaaa ttgcatgaaa ataatttcaa atgagcgttt
       61 tttgatactt tagagtgttt cctacccttt aatatcaccg tgtagagttg atatcactac
      121 aacagaaaca tgcatgtcat ttgtttactt ctcattttac tacagaaaaa tttatctatt
      181 tttctaaaca tcagtgaaat actcataaaa ggtaactatt atgtctatgc cattaagcaa
      241 tacccttcag agccaaatta ttacagataa tcacttcctt catcatccaa aagtcgacag
      301 tgagttaaca cgtaaatatg aacgtgcacg cctggatacg gaaaatatct atcttctgcc
      361 tcttgctcgt ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaaact
      421 tgatattagt aaagagtcct ggccatttaa ttatgtgaca gaagcatgca gagaatccga
      481 tggtattaca acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga
      541 cgaaatatat ggtggcatat gtaaaaaggc gcatgctgct acagaattag cagaaggttt
      601 acggctaaat ttatttatga agagcccttt cgatcctgtt gagaactata cagttcatga
      661 aataacatta ggccctggtt gcaatgttcc aggttacgcc ggaaccacga taggatatat
      721 ttcaaccctt ccagtttctc aggcaaaaag atggaccaat gagcagccaa gaattgatat
      781 ctatattgat cagataataa ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc
      841 cgcattactt aatgctaata ttgagatggg aaatgaccca atcattggta tagaagccta
      901 tcctggtacc gcggagatcc atgcaaaaat gggatataaa gttatccccg gtgatgaaga
      961 tgcgccactc aaacgcatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa
     1021 aaatggtgaa tggaattata ttggtaaata gccactgaga agaatgagta ctaatattca
     1081 ggttatacag atatttaaac aataccctgc aatttacaaa gcaatactca ttgcgacttc
     1141 ttaatttaag aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcagacgtc
     1201 gactttatcc agcggccatg aatcaaacag atagccgtca aaataaatat cgtcaacgtt
     1261 cgagaattcc agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc
     1321 cgctcgggca tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc
     1381 tttgcgatac aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc
     1441 ccgccactgc caggactgac ggaataactc caccagcatg ctgttatgcg ttgcttcggc
     1501 aatagcgaga tggaactgca tgtcaccgct ttcgctgctg cccggcgcac tggaagccag
     1561 ttcacgctct tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg
     1621 caaagcggca aactcggcga tgttgctctc caataactgc cgcgcctgta acagctcaaa
     1681 aggaccggca tcgttgcaga cgttggcatc cggactgtct gtgttctggc tgcctgagtt
     1741 atcaagaaca tagataccgg caccccggcg tacttccacc agccctttga tctccagcat
     1801 gatcagcgct tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc
     1861 cggcggcagc cgttcgccag gattgtacgg cgtctttatg atcagatcgc ggatcatcgc
     1921 cccgacttcc tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt
     1981 aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg
     2041 ggcaatttta cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt
     2101 acaggtgggc aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt
     2161 ctgtagggta atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca
     2221 gctgctgata tgccgccgtc actgcgccaa caaagtcggc attctgcggc agatcatcgg
     2281 caaaaatacc gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt
     2341 tgaccttctg gaactccgcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat
     2401 ccacgccctg ggtgtaacgc atccagccag ccacgcccag cgccaggtga cgccagctgc
     2461 cgccgttttg caggtgcaga cgcaccgggt ccagcagacg ctgcggtaac ttctggctgc
     2521 cgtccatcgc aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca
     2581 gcagcgtcgc ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt
     2641 cctgcatcat caaggagaag gccgctttgc gataatccgg gttagtcatg gtgtcggcaa
     2701 tggtttcata gccgccgagg taaccgaggt atgccaggaa agagtggctg ccgttcagca
     2761 tacgcagctt catcatttcg aacggcacaa cgtctgcaac gaactgtgcg cccactttat
     2821 cccagtccgg gcgaccgtta acgaagttat cttcaatcac ccactgacgg aacggttcgc
     2881 aggcaatggc gcacgggtcg taaacaccca gctggtcagc aatttcctgc aaggtttctg
     2941 gcgtcgccgc cggaacgatg cggtcaacca tggtgcacgg gaaggtgacg ttttcttcaa
     3001 tccatgccgc cagctgcggg tcacgcgcct gagccagtcc cagtaccgcg accttcgcca
     3061 catggccgtt ttcacgcacg ttatcgcagg acatcaccgt aaacgctttc aacccttttt
     3121 cacgacgcag ccgcagggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt
     3181 tttccagatc gtgcttgatc agtgggttat tgagatccag ctgaccgctt gccgcatcag
     3241 cgcagtagcc tttttccgtg actgtcagag agacaatcgc cgtctgcgga cgcgccatcg
     3301 cattgagaat accttcgcag ccgtcgattt ccggatgcag cgcttctttc atcgaaccga
     3361 taattttcag ctcggtgctt tccgcgcctt tttccgccac ggtgtacagc agttgctgtt
     3421 ttttcaggtt ttcgatcagc acgcggtcgt tgcctggcat caggttaact tcgcagatac
     3481 cccagtcgct gtcggtgctt tccagcaggt gatgggtata cagcgcctgg tgcgcgcggt
     3541 gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag acgagaatgc tcccatgacg
     3601 ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc
     3661 ttcagagagt gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtgggta
     3721 ttaacggcta aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc
     3781 tttcagacga ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat
     3841 ctgatgaccg tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg
     3901 gtgttcttct tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg
     3961 cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa
     4021 gtaaatacgc ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc
     4081 gtaggaaccg gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca
     4141 ctgcatatct tcaatggtgg aaacaatgcg cggcaggcca aggatcgggc gcggcggatc
     4201 gtccgggtga accgccatac gcacgccaac ttcttcagca actggaataa tcgctttcag
     4261 gaagacggca aagttttcgc gcagcttcgc tttatcgata tctttgtaca gctccaggtg
     4321 cttacggaac tggtcgaggg tatacccttc ttccgcgccc ggcagaccgg caatgatgtt
     4381 acgcgtcaga cgcgctttgt cttcgtcgct catagtggcg aagcgaacag cagcctgagc
     4441 aatttcttct tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa
     4501 tgcagcgaat tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc
     4561 gaggtcagta cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc
     4621 gcactgcgcc aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc
     4681 agtgtgggtt ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc
     4741 tgcgtcttca acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt
     4801 cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa
     4861 agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc
     4921 acatagtggg ttccacagca aagagacgcg gaaccctgga gttgtttagg tatccggatg
     4981 aacaaccaga ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt
     5041 tggtaagaca tgtcaacccg aattctgaaa ttggttaacc acatcacaag aatttcactc
     5101 tcatggttgt tgctgcaaat tgcagcaaag ggtaatcgat ggttattgat cagaggatgt
     5161 gacattcatc gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg
     5221 ttcaaccagg taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt ggtattcggc
     5281 attggcctga tgctggtact gacaaagacc gcaggttcag acacattcgc tgcattagca
     5341 atgcctctta gataattttg acctgtcagt aaaagacacg ttgaaaacct ggggtctgct
     5401 ggagctggtc aactccgtgg ttgggctgat tattagcaag gtagcgtaaa agaacaccag
     5461 agcctgccaa tggcaggctc tgactgatga caaagtctaa aaatgtgccc ggacagtccc
     5521 ctcgcccctc cggggagagg gttagggtga ggggaacaga ccagcaaagt gcgctttgtt
     5581 tatgccagat gcgacgctga cgcgtcttat ctggcctaca aagggctaac gtgcaggttt
     5641 ttagcttcag gtaatattgc gtaactgcat tagcaatgcc ctgtgatttc tttattgata
     5701 aacaaaagtc acgccaataa tcgattgcac attccctgca gtcacctgcc ctccggtacg
     5761 tgcgtaattt gccgttaatc ccagacttac cgccgaagtc cctactgctc ctaacgatac
     5821 cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc
     5881 tggtgaaaac gacgcggtat tggtgaaaat cgagttgccc gcatctacgg ttgtgccgga
     5941 gaggtaatac cccagatttt ggcttttcgc acaataaacg gtaagaggaa ttggcactga
     6001 accagggtag tccggcagag taacggtgac atcacgagca gaaacatcgc agccgccagt
     6061 aggcaccacc acatcattat tggcgtaaat attccacaca aactgaaaat catcgctgtt
     6121 atagttgttg gtctgtcgca aaataagcac ggcaattaat gagccagctt taatcgccac
     6181 cccgcccgca ctgctcacag gcgtcaaata aagcgccacc ggccacggct tatccgttct
     6241 cgaattataa acaacgcgcg gcgtttcgct ggtggtagga aatggatcgc tactgccact
     6301 atattttacg gtcccggaaa aattagataa cacgctgcca taagccgagc ctcgttgcag
     6361 tgtgacatag tctgtaatgg tttccggata atcgttatgg caaaagattt gcgtcgaaag
     6421 atccacgacc aggttttgcc ccacattcac ggcgggcgca aggtttacat aaacattggc
     6481 gctgccaccg ccaataggga tagcggtacc attggcggtt ttacaggcga atgaccaggc
     6541 atttaccgac cagcccatca gcagtacagc aaacagggta ataactcgtt tcattacaat
     6601 catctcttcg ggttcagctg taggtatagg tgatgctaat cactgcctga atggttccct
     6661 gagtggctcc gccatttacc gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg
     6721 aggaatcatc cacctgaact gttttggttg cgccagtatt caatgtgttg ccactgtcat
     6781 cctgtagctc taactggatg ttttgcgcgg tcccctggtt tttataatat ccggtactgt
     6841 cggctgcccc gctgaagctg gcggtgaccc tcgacgttcc caccggacaa ttagtcaact
     6901 caagcgcaac atcatgccag gccgatgccg ccccggcaga catcagactg aaagaataaa
     6961 gatcgccgag atcaaccgtg gcattggtgg tggaaaccgt acacggtttg gcgacgacct
     7021 taccgttcac cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg agcgccaata
     7081 ttgccgccaa taaataccca cgtttgcgcc atttcatgag catctccagt tactgatatt
     7141 caagagtgaa ggtagccgtg gcattgatat gccccgcagt gacaggcacc tgtgtcgcca
     7201 ttagccgggc gtaaaaattc aacgtatttg gtttacccgg cgtcagggtc gtccacgaaa
     7261 tcgcggacga tggagcatta aggggtattt gattttgctg ctcattcaga agctgtattc
     7321 ccagtcccga agccgctgac accgtatttt caagtgcaag cagattggca ttgtggctat
     7381 ctgcaacgcc ggtaaaccca acttttacgg cagaaacggc attaccacag ggtgacagca
     7441 aaatacgaaa tggaacaaca ggagtcgtcg cgccaatgtt gttaaattgc ttcgccgcgt
     7501 tttccatcag atcaacagta aaattggttg attcagcggc cacactacag ccgttatctc
     7561 tgacatagcc gcggatagta atcgtgctat ccgcagccaa agcgtgactc accgccagcc
     7621 acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat cacgccccct taacgacatt
     7681 cagctgatag ctgggttaat aactgctgct gactctctgg tggcagttga taattggcga
     7741 cacagtgagc attttcctct tctccccatt tcacctgaac ttttcccgct aaaggcattc
     7801 cgctgaggta aacctgacca ttatccgcaa caatgccgct actctggcta ctctctgatg
     7861 tcaccatcgc cccaaacggc agcggcttat tattgtgggt tagcgtcatg agcagtttta
     7921 tcccaacgcg cgctttaaac tctgctcgca cgatcgcccc acgagtggga acaacgttag
     7981 cgaccgcgtt atctaaatcg acgttatcag ccagggtatt ggtatccagc gccactctgt
     8041 tttccctata ttcagtggca taaggcagca cggcataacc gcgccagtcg gtacgcaccc
     8101 ccgtctggtt ttcgactttt gcatcttttg cgccaggcgc tttaacaagc accaccgtat
     8161 cgtttaacgg ctgccccagc gttacgccat tggcatgagc caatacccca ccgctgactc
     8221 cgtaatagag ctgcttaata tcatcgctat ggctgtaacc gatattggca ttgccgtaac
     8281 caccgcgata attcagcgtg gcgtagcctg tgcttccgct attaccatcg cctcccccgg
     8341 catagccggt ttgcacgcta tagctgaggt tgttgtcttc cagcaacgta ccgtatacac
     8401 cagccagatt ggtcatccga ccgttgagat cgtgtgacat gctgtagctg gcactggcat
     8461 gtcgccactg agatttactg tcagaacgca gccagtggct gaaaggaata ttgacgttaa
     8521 gcgctaacat ctgatcacgt cctttttgcc aggcgttttt cgtcaggcta tagctgagcg
     8581 tccagttgat atcttcgaac gcagtattta atccagcctg gaattgctca tcgacattac
     8641 tcgttcccca ataagtttga tggctaccac tcaaatacag tgttgatgtg cgcccgagtt
     8701 gctgagtaac ggtgagctgt aatttcccgc gtttgttata agcgaggttg taatagtcgg
     8761 tgaatttcgg cttaacctga ataactccat cctgtgtttc gatgttgtag ccattcattc
     8821 gactgtatgt tgtatcagcg aaattaaaat atccgctggt cgaataacgg taacccacta
     8881 actgaatatt cgtgcctgat tcattgagcg atttgttata gagaaaacgc accgattgtc
     8941 cgtcatgctg actgtcatcg ggaagtgtgg aattagcctg cgtcatatcc acagacagag
     9001 cgcccagtgc ccccatgttt ttcccgatac cgaaattaaa agcacgataa cgatccgcca
     9061 gttgcgttcc accatatatt gtccagccag ccggaaggcc gtggagtaat gtactctgga
     9121 aaaagcgggg tttttcctgc tgcgcatttc cactacggta ttctcctgcc gtaatggaat
     9181 aacgagtatg cccttcacgt tgcaaaagcg ggactgacga atagggtacg gtaaaaatct
     9241 gcgtgctgcc gtcagcctct ttgatcgtta cctgcaagtc accactatta cctgcggcat
     9301 agatatcgtt gatggtaaaa ggccccggtg gcaccgtact attataaatg tcatacccat
     9361 tttgtttaat agtgacctgt gcagtaccac gagcaatacc gtggatcacc ggggcaaatc
     9421 ctctttggct atcgggtaac atattgtcat ctgaggccaa ttgtgcgccg cgaaagttaa
     9481 taccatcgaa aatatcgccc tgagtataac catcacccag cgtcagccgg gaacgtaacg
     9541 gtattatgtc tcgctcaagc caggtattga tatgctgcca tttatttttg ctacctgatg
     9601 atctgtcgct actgttataa ctccaggtgg tattgtcgcg taaacgccac gcaccaatat
     9661 ttaacccact ctgtaggttt aaatatgcat aatggctgtt acccccaatc cgattctgta
     9721 cactatttcc gctgaaatta taattgagca atccggcatt aataccggga tcccataact
     9781 caggaggaat ataaccacgc gcgcgattac tcataaatgc ctgagggatc gtcaggttca
     9841 gtcgctgctg accaacatcc agatgcgcag tagcgtcctg gaccattgtg gttaatggca
     9901 cacaggcatc atccgccagc agattcatac cggcgacaga agccgtattc agccccatac
     9961 tggcgagttg cgcgcgtgtc aggcagggaa caatcccttg ttcactgtcg cccgtattaa
    10021 atgtgacatc acgcgttgcc atataaccat tattcaaata gatatcgacg cgatacgtcc
    10081 ctggcggtaa ttcttgccca ttttcaaaac gcgataaatc ggccacagcc tggggatcat
    10141 ccgctaaaaa acgcgggtta aaatagagtt cggcagatga caaaggtgcc tgtgcggcaa
    10201 aagcacaggc aacaaagagc cggacaaaaa aaccagccaa acgatgctta cgaatatgca
    10261 agcattgtgt gtttcgctgg taaagtctta aattcagata tgacattatt cctccggttt
    10321 aaagtcaccc ggcagtcaat tctttttatt caggcgaaaa attccccctg cgttattcca
    10381 ttacgcccgt cattttgggg gtaagtgcac cataatcatt tattgttcgg taagtaatat
    10441 tgcttcccgc atcagaaggc aatttaaccg tgctttcgcc cattggaggc accaaggcat
    10501 tttcaagaac ccgggttccg gcattcaact ctgttaccgt caggtaatag ggtgtcgggt
    10561 taatcagcgt cagagaattc gcgctacgac gaaatcttaa tttttctgcg gcctgatcgg
    10621 gtggcaacgc taatttagcc gggcgatagt acagtttaat gcggctgata attgcgagct
    10681 gtagcgtatt ctcagtcaat tttgatttat ccattgacgg aatcgcttta acattcatcc
    10741 agaataaact ttcccgatcc tgtggcaatt ggttatttgt tgcatcaaga atacgcaagg
    10801 tattctcttt ttttcccttc atcgcaaaca gaggaggcgt cacgataaaa cgaccatcct
    10861 ttacaccatc ggcattttcc acccatgatt gaattaaata ggtactattt tcatcattat
    10921 ttgtcacggc aagttgcact tgtttttgcc ctgccggata aattacgcga gtcgcaccta
    10981 aggccactcc cgcttcagcg cgtccggcaa ccatcattgc cataaacatc aggatacctg
    11041 ccagcaagca gaatgttatt tcctgcgatt tccttacatt gacgttttta ttactcactg
    11101 tcctgttcct gttatcacat tatctgctga acaattactg ataggttaaa gagaaccagg
    11161 cctgggcatt ggcgatgccg ccagtaaccc gacgcccggt agcacgatat ttggcgatga
    11221 aatgtagcga agtagagcct gagtaaagcc gtttccagtt tgctggagga cgattaatcg
    11281 gtacgaggtt tccttcatca tcaaacaacg ctacgccaat attggtggct atccctggcc
    11341 cctctcccac ggaaagcaca tccggatttt taccatccgc gacaccgtga aacgccaccc
    11401 ccacacgttc actcaccacc gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg
    11461 cgctatcttc ccctgccgca tgaaaccggt tactgctgat ttgccccata ttgaccgtca
    11521 tttgtttatc accggcttca atccggcaag tttccgcaat aatgacgccc tgaaattgca
    11581 tatttccgcc gggcagcgtg gtattccatt tatttccggc cagagcaaac attggcagca
    11641 acgacgctaa cagaaataat ctcttccttt tcatcgttat ttttatcgca caagggtggg
    11701 catccctgcc cgtaatgacg tccttgaacc tgggtaggtt attgatactg aaccttgaag
    11761 gttgcatccg cattagctgc acccggggtt gcctcgccga ttgcataata acgcgcctgg
    11821 aacggaatgg tgttggtacc gttattcagg gttgtttgtg cactaaatgt cgcaccgtcc
    11881 agcgtcagag cattgcctgt tctgtccagg atctgcacgc caacgtttgt tgcactacct
    11941 gcagcagaac tctgcagagc cagtacatca gtacgcgtag catcaattgc cgtacctaag
    12001 aaggcaacag cggcttttgt ggcaacagtg gtatcgcaat cattcaactg aatgttaaaa
    12061 ccaacggcag agctggttgc tccagcctgc ttcaggctag cggtacgaac ctgtcccaac
    12121 tgaacggttt gatcaacaga gcctgcatca actgcgcaag cggcgttaac aacttcccct
    12181 ttaaagtgaa cggtcccacc atttaccgtc gtagtatcgg ccagagccgc tgcggaactg
    12241 agggacagag ccgacagaac aacgattgcc agagttttaa ttttcatgct gctttccttt
    12301 aaaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga
    12361 gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg ggccattttg
    12421 actcatagag gaaagcatcg cgcacaaact ttttcagttt atttgttggc ttaatattct
    12481 attgttatct ttatttatag atttttatat tgcatgaggt ggtttttgga gagaagaatg
    12541 aggaagatgc gtcgagccac agaaacgtta gctttacata tagcggaggt gatgtgaa
//
LOCUS       DAZFKM010000142        12494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFKM010000142 DAZFKM010000000
VERSION     DAZFKM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..315)
                     /gene="yehE"
                     /locus_tag="ACHKAY_004191"
     CDS             complement(34..315)
                     /gene="yehE"
                     /locus_tag="ACHKAY_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO5702768.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(578..1687)
                     /gene="apbC"
                     /locus_tag="ACHKAY_004192"
     CDS             complement(578..1687)
                     /gene="apbC"
                     /locus_tag="ACHKAY_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO5702769.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1819..3852
                     /gene="metG"
                     /locus_tag="ACHKAY_004193"
     CDS             1819..3852
                     /gene="metG"
                     /locus_tag="ACHKAY_004193"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO5702770.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3993..7787
                     /locus_tag="ACHKAY_004194"
                     /pseudo
     CDS             3993..7787
                     /locus_tag="ACHKAY_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7797..11429
                     /gene="yehI"
                     /locus_tag="ACHKAY_004195"
     CDS             7797..11429
                     /gene="yehI"
                     /locus_tag="ACHKAY_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO5702771.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11491..11808
                     /gene="yehK"
                     /locus_tag="ACHKAY_004196"
     CDS             11491..11808
                     /gene="yehK"
                     /locus_tag="ACHKAY_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO5702772.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3273 a         3168 c         3062 g         2991 t
ORIGIN      
        1 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
       61 acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga
      121 aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc
      181 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc
      241 agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag
      301 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      361 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      421 gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      481 attaaataag tcttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct
      541 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      601 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      661 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      721 gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg tggtattttt
      781 cagccagttt ctcagcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      841 aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca
      901 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
      961 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1021 tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg
     1081 ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa
     1141 ttgaattggt tgccaggcca tgagacataa tcggtgccat gtgggtaccg tccggcgacg
     1201 ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt
     1261 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1321 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa
     1381 tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1441 tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa
     1501 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1561 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1621 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1681 cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa
     1741 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1801 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1861 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1921 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     1981 cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga gcagatgatt
     2041 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2101 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2161 aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2221 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2281 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg
     2341 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2401 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2461 gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc
     2521 gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2581 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2641 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2701 ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac
     2761 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg
     2821 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2881 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     2941 ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc
     3001 tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaactggct
     3061 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3121 agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3181 tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg
     3241 caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg
     3301 gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat
     3361 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc
     3421 atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct
     3481 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3541 gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt
     3601 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3661 ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg
     3721 gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct
     3781 ggtcctggcg ggaaagatat tttcctgcta agcccggatg ccggtgctaa accgggtcat
     3841 caggtgaaat aatccccctt caaggcgctg catcgacagc gccttttctt tataaattcc
     3901 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatcag
     3961 cattcaactg gataaaatta cagggatgca gaatgaaaca ctttatctat caggacgaaa
     4021 aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc agttggggaa
     4081 aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg gcagcggcaa
     4141 aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg
     4201 ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca
     4261 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag
     4321 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4381 acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4441 cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg
     4501 acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4561 acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4621 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4681 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4741 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4801 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4861 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatga cggcaaccat
     4921 taattgcttt gttattacct gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     4981 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5041 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     5101 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5161 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5221 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cggatgacga
     5281 aagccattgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa
     5341 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg
     5401 cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc
     5461 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct
     5521 cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5581 taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5641 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5701 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5761 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5821 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5881 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac gccatgttac
     5941 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6001 ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct
     6061 tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac
     6121 atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg
     6181 cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg
     6241 cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc
     6301 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6361 ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc
     6421 tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga
     6481 aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc
     6541 agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta
     6601 acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt gaatcccagc
     6661 ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta
     6721 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6781 aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt
     6841 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc
     6901 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc
     6961 gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga atcgcggtcg aacgatgcgg
     7021 ttaaccgcta caaattgctg aaaaaagatg cgcgtaccgt cgccgcccag caggtagcaa
     7081 ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgcc ggaaaacttc cagctcttcc
     7141 tggttgagca tccgctggtt cgccacttaa cccgccgtct gatttggggc gtttatagcg
     7201 ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg
     7261 acgatgatct tttcaccctg ccggaaggcg atatctctat cggcattcct cacgttctgg
     7321 agatatcacc gacggatgcc gccgcctttg ggcagctttt tgccgactat gaactgttac
     7381 cgccgttccg ccagctcgac cgtaacagtt acgccctgac agaaaccgag cgcaatgcca
     7441 gcgaattgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga
     7501 ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg atgatcaaac
     7561 ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc
     7621 cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa ggcaaagcag
     7681 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     7741 tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7801 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7861 aaacgccgct cagtcatcgt cgctggccgg gcgaaccagt gtccgttatc actggaagtc
     7921 tcatccagac attgggagat gaattactac aacaagcagg ccagaaggaa aatatcacct
     7981 ggaattatga taaatgctca ctggagtggc agtccgccat ccagcaggca atcaatttga
     8041 ctggcgagca taaaccgtca atcccagccc ttaccatggc ggctctgatc tgtatcgcgc
     8101 aaaatgacag ccagcagtta cttgatgaaa tcgtgcaaca agaggggctg gaatatgcga
     8161 cggatgtagt gatagcgcgt cagtgcattg cgcgacgtta tgagagtgac tcactggtcg
     8221 tcactttgca gtatcaggac gaggactatg gctacggtta tggctccgcg acctataacg
     8281 atttcgatct ccgtttgcgt aagcatctgt cgctggcaga ggaaagttgc tggcagcgtt
     8341 gtgccgataa actcattgcc gcactgccag gaatacccaa aattcgccgt ccttttattg
     8401 cgctgatcct cccggaaaaa cctgaaatag ccaatgaact cgccagtctt gaaagctctc
     8461 ggtcaagtct ccattcaaag gagtggttaa aggtcgttgc tactgataat acggcggtaa
     8521 aaaaactcga acgatactgg ggcctggacg tatttagcga tcgcgaagcc agctacatgt
     8581 cacaagaaaa ccgcttcggc tacgcggcct gcgcctccct tttgcgcgaa caaggactgg
     8641 cagccgttcc gcgcctggca atgtatgccc ataaagaaga ttgcggcagt ctgctggtac
     8701 aaattaacca tccgcaagtc atccgcacct tgctactggt ggctgataaa aacaaaccca
     8761 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca cgcgacgctc gccgcactgg
     8821 cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc atcgaagaca
     8881 aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc
     8941 tgatggcatc gcagccacaa ctggcagcag aagtgatgcc gtggttaagt actcaagcca
     9001 gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat agcaccgata
     9061 acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9121 ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg caaccaggcg
     9181 aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg
     9241 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9301 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca
     9361 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9421 cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9481 tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9541 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc attgagaaaa
     9601 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9661 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9721 gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg
     9781 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9841 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9901 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
     9961 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10021 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10081 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10141 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10201 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10261 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10321 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10381 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10441 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
    10501 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10561 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10621 acctcaaccc ggttatcttt aatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    10681 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10741 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    10801 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
    10861 tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca
    10921 aagcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
    10981 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagta gaaatgcgca
    11041 gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc cagttgtcgc
    11101 gccgcacggt gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag
    11161 gtaaatccgc taccgttggg cagcttatgg gaatgcgata caaaggctgg gagtcaggtt
    11221 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    11281 gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgc
    11341 accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11401 taagcgcacc tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt
    11461 tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt
    11521 tacgattatg agatccctgt acctgaaaat ccgttttcct tcagacttga gatccataaa
    11581 tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct acgtccaaca
    11641 tttcatcaac gtgatcgaga agatactgac ccgctaataa atgatgcgtt gatttatata
    11701 agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca
    11761 atttttaatc gtgaactaca gaatatattc aaccaagaaa tagagtaata tactctagat
    11821 aattaaagtc gcagcacatg caacttgaag tatgacgagt atagctttct taaaggggga
    11881 aacggtattc ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc
    11941 gtaacagagc cactaataac tccagtacgg caatgagcac ggttaacttt tgtgtcagcg
    12001 tgtccatccg ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg
    12061 ttgatactgg tttacgtatg ttggtacgaa acgttgctct ccggatcagg gcaccgcctg
    12121 cggcaccacc cgtccggcag ccaggactta aaattggtcc gatccggcac aacctcccaa
    12181 cgggcaggat tataaatctc actcactgaa ttgcaatgga gtgtggatat ggttataatt
    12241 tattcgttgg tacgaaatgt tgcacaatca gcaagttagc gtcactgtag taaataaaag
    12301 aacctgcctc accagcaggt ttttttattt tctgcgatct actttccaga tattttttgc
    12361 caatagccca acataaatat tcacatcccg gtcaaatggt gcgttgaata taaaatcgat
    12421 gaatttataa ataaaaatga gttatatgat gaattaataa aaacagtaat cattttatta
    12481 aagggggaag cggt
//
LOCUS       DAZFKM010000143        12467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFKM010000143 DAZFKM010000000
VERSION     DAZFKM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(155..1579)
                     /gene="patD"
                     /locus_tag="ACHKAY_004197"
     CDS             complement(155..1579)
                     /gene="patD"
                     /locus_tag="ACHKAY_004197"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO5702773.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1601..2395)
                     /gene="ydcV"
                     /locus_tag="ACHKAY_004198"
     CDS             complement(1601..2395)
                     /gene="ydcV"
                     /locus_tag="ACHKAY_004198"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5702774.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2385..3326)
                     /gene="ydcU"
                     /locus_tag="ACHKAY_004199"
     CDS             complement(2385..3326)
                     /gene="ydcU"
                     /locus_tag="ACHKAY_004199"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5702775.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3327..4340)
                     /gene="ydcT"
                     /locus_tag="ACHKAY_004200"
     CDS             complement(3327..4340)
                     /gene="ydcT"
                     /locus_tag="ACHKAY_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO5702776.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4358..5503)
                     /gene="ydcS"
                     /locus_tag="ACHKAY_004201"
     CDS             complement(4358..5503)
                     /gene="ydcS"
                     /locus_tag="ACHKAY_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO5702777.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5748..7153)
                     /gene="ydcR"
                     /locus_tag="ACHKAY_004202"
                     /pseudo
     CDS             complement(5748..7153)
                     /gene="ydcR"
                     /locus_tag="ACHKAY_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7232..7648)
                     /gene="hicB"
                     /locus_tag="ACHKAY_004203"
     CDS             complement(7232..7648)
                     /gene="hicB"
                     /locus_tag="ACHKAY_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO5702778.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7694..7870)
                     /gene="hicA"
                     /locus_tag="ACHKAY_004204"
     CDS             complement(7694..7870)
                     /gene="hicA"
                     /locus_tag="ACHKAY_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO5702779.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8092..8322
                     /gene="yncJ"
                     /locus_tag="ACHKAY_004205"
     CDS             8092..8322
                     /gene="yncJ"
                     /locus_tag="ACHKAY_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO5702780.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8414..10375)
                     /gene="rlhA"
                     /locus_tag="ACHKAY_004206"
     CDS             complement(8414..10375)
                     /gene="rlhA"
                     /locus_tag="ACHKAY_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO5702781.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGSGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10448..10984)
                     /gene="sutR"
                     /locus_tag="ACHKAY_004207"
     CDS             complement(10448..10984)
                     /gene="sutR"
                     /locus_tag="ACHKAY_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO5702782.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            11037..12251
                     /gene="ydcO"
                     /locus_tag="ACHKAY_004208"
     CDS             11037..12251
                     /gene="ydcO"
                     /locus_tag="ACHKAY_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO5702783.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12291..>12467)
                     /locus_tag="ACHKAY_004209"
     CDS             complement(12291..>12467)
                     /locus_tag="ACHKAY_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO5702784.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3051 a         3347 c         3055 g         3014 t
ORIGIN      
        1 tgctaatcaa ctgtgacagg atgaaaaatc gccatcaaac atttagccta atttttacgc
       61 tagttattaa tgtaatcatt ctaatactta accgccatta aactcagaac ctaaaacgat
      121 agtggccgtc ctataacagc cactaacatc taacttaatg tttaaccatg acgtggcgga
      181 cgacggtgta atcctccagc ccataaagtg acatatcctt gccgtaacca gaaagtttct
      241 gcccaccgtg cggcatttca cttaccagca tgaaatgggt attgacccag gtacaaccat
      301 attgcagccg tgcgctgacg cgatgcgccc tgcccacatc tttcgtccat accgaagatg
      361 caagtccgta ctggctgtca ttcgcccagt tcaccacctg ttcttcgttg tcgaagggcg
      421 taacactcac tactggacca aatacctctt tttgcacgat ggcatcgtcc tgtaatgcgc
      481 cagccagcag cgtcggcgca tagtaatagc cattaccctt gcgcttttca ccgccagtga
      541 tcactttgat gtgccctgtc gctttcgcct cttctactgc cttgctgacg cgttcgagat
      601 gcgccagcga gcttaaaggt ccaagctccg tagactcgtc atccggcgca ccagatttta
      661 acgttgccac cgcagcaccc agtttttcca ccagcgtatc gtaaatgcct ttttgcgcgt
      721 agatccgaca agccgcagta caatcctgtc cagcattgta atagccaaat gtacgtacac
      781 cttcgaccac tgcttcaata tccgcatcat caaaaacaat cactggcgct ttgccaccaa
      841 gttccatatg agtacgctta atggacgacg cggtatggct gatgatgtgc tcgccggtgg
      901 cgatagagcc cgtcagcgac accatccgca ctttgggatg accggtcagc ggatcaccca
      961 ccgttttgcc tctgccaaac agtacgttaa tcacgcctgc cgggaagata tctttcgcca
     1021 gctctgccaa cttcaacgcg gtcagcgggg taatttctga tggtttaagc actacgcagt
     1081 tccctgccgc cagcgccgga gccagtttcc acgctgccat catcagcgga taattccacg
     1141 gtgcgataga agccacgacc cccaacggat cgcgacggat catcgaagta tgaccttcaa
     1201 gatattcacc tgccgccaga ccattcagac agcgcgccgc acccgcgaaa aagcgaaaaa
     1261 catcgacaat cgccgggatt tcatcattga acgcactatg cagcggtttg ccacaattac
     1321 gggactccag ttcggcaaaa acctgaccat tttcttcgat aacatcagcc agtttcagca
     1381 gacattccgc acgcactttc ggcgtggttt gcccccattc ggcaaatgct gcatctgccg
     1441 cgcgcacagc agcatcgacc tgctctgcgg atgcctcggc aatctccagt aaaacgtccc
     1501 ccgttgccgg attatagaca ggctgttttt ccccttcgcc gctaaccagt tctccgttaa
     1561 tcagtaactt atgttgcata gcatattcct gtatcagtgg ttattgacca ttgtcgcctt
     1621 cgcgggttag ccaccaggcc cccaggatcg gcaaggttgt taccaacata accagcagtg
     1681 ccaccacgtt agttaccggt acatcacgcg gtcgcccaag ctgattgagc aaccacaacg
     1741 gtaacgttcg ttcatgacct gccgtaaaag tcgtaacgat gatttcatcg aacgacaagg
     1801 caaacgccag cattcctcct gccagtagcg ccgaactgag attcggcaac actacgtagc
     1861 ggaaggtttg ccagccattg gccccaagat ccattgacgc ctcaaccaga ctccacgagg
     1921 tgcggcgaaa acgggcgatg acattgttaa acaccaccac tacgcaaaaa gtcgcatgac
     1981 cgaccacgat ggtgaaaaat cccggctcca gattgatggt tttaaaagcg gttaatagcg
     2041 ccagaccagt gacaatgccc ggcagcgcaa tgggcagcag cagtaacagc gaaatggcgt
     2101 ttttgccgaa aaagtctcgt cgccacagcg cggcagctgc cagcgtccct aacaccagcg
     2161 ccattaatgt cgccagcgcc gccactttaa gtgacagtgt cacggcatca agaatatcac
     2221 tacgctgtgc tgccacgcta aaccagcgca gcgtcaggcc ctgcggtgga aaactaaacg
     2281 ccgcatcttc agtgttaaac gcataagcgg cgataatcag gatgggaaaa tgtaggaaaa
     2341 ccacgccgcc ccaggccgcc agtttgagga aaaacggtgc gcgttcagag tgcatcgaac
     2401 gctcccagac gtttcacgaa cgccaggtac agtgcgatga gaacaatcgg caccagggtg
     2461 aatgccgccg ccatcggcat attgccaatc gccccctgct gggaataaac catattgccg
     2521 ataaaatatc ctggaggacc aaccagctgc gggacgataa aatcgcccag tgtgagtgag
     2581 aaggtaaaga tagagccagc ggcaataccc gggattgcca gcggcagcac cacatagcga
     2641 aaggtttgtc gtggacgtgc gccgagatca gccgacgcct gcaacaatga cggcggcaaa
     2701 cgctcaagcg ccgcctgaac gggcaggatc atgaacggca accagatata gagaaacacc
     2761 agaaagcgcc ccagcccgga agttgacagc gtatttccgc ccaccgcagg taatgtaagg
     2821 aacgcagtca gcagtggttc cagcccaaga tgttgtaaaa accactgagc cacgccatct
     2881 tttgccagca ataacgtcca ggcataggct ttaacaatgt agctcgccca catcggcaac
     2941 attaccgcaa tataaaaaaa agctttcatt ttcccgctgg tatagcgcgc catataccac
     3001 gccattggaa aagccagaat ggcgctggcg atagtgactg ccacagccat agtcagcgtg
     3061 cggagaatga tgtcgtaatt cgccggatta aacagcgcac ggatattcgc cagcgtcagt
     3121 tccggcgtta ccgacatggt gaaatcgtca aaagtataaa atccctgcca taacagtgtc
     3181 agcagcgagc cgaaatagac aatgccaaac cacattagcg ggccaagcag cagtaaaaac
     3241 agccccagcc tcggattacg ccagaagaaa ccggacacct tacccagacc tggacgtgaa
     3301 ggtgattgca atacattcat cgccattcac ctctcctcaa ccagcggcac catcacatca
     3361 cgcgaccagg aaaccatcac ctgttgtccg ggcgtgagcg tggcaggcag ttcttcgcct
     3421 gtcatattgg cctgactcac aagcagtttt tcaccgccgt tcaatttcag ttcaaaacga
     3481 gttgccgcgc cctgatattg caccgcctgg atcgtgccat tggcctgcag ttcaccaggg
     3541 gtgttgaggc ggatatgttc cggtcgcagg gcgaagcttc ccgtcatgcc acaaagtttc
     3601 tctgccatca gtccatcaaa aacgttggat gtcccgacga atccggcaac aaacggcgtg
     3661 cgcgggcgca tatagagatc gcgcggggaa tcgacctgct caatgcgtcc attattgaaa
     3721 accgccacac gatcggacat cgataacgct tcgccctgat cgtgggtaac gaagataaaa
     3781 gtgataccga gagactgttg cagttttttc agttccagct gcatctgctc acgcaatttg
     3841 agatccagtg cgccgagcgg ttcatccaac agcaatacgc gcggttcatt caccaatgct
     3901 ctggcgatag caacccgctg gcgctgacca ccagaaagtt gtgacggttt acgttgatgt
     3961 acaaacccca acgccacttt ctccagcgcc tcttgcgcca ttgcgtgccg ctgcttttta
     4021 ttcacgcctt tgaccatcag cccataggcg acattgtcaa gaatcgacat atgcggaaat
     4081 agcgcgtagt cctgaaagac agtattcacg tcccgctccc acggtggcag attgctggcg
     4141 ggtttaccaa agatagagat agccccgccg gaaagctgtt cgaagccagc aatcaggcgc
     4201 aggcaggtgg ttttgccgga gccggacggc cccagcatag agaagaactc accatctttt
     4261 atcgcaatac tgacgccatc tactgcgcgc acgtcaccgt acaaccgcga gacgttgtca
     4321 aactccactg cgtacgtcat aaagcacccc agcgaagtta gcgaccgccc ataatggcaa
     4381 tgtaatcctg cgtccagcga ctgtagggaa caaacttgcc cccttctgct ataggcgttt
     4441 tccagaaggc gattttgtcg aaatagttaa aaccgttggt ttcacaacct ttttcgccta
     4501 ataacggact ggctttacac ccttccggca ctaccggtaa cgagccaaac caggccgcca
     4561 catcgccctg cacttttggc gttaatgacc agttcatcca tttgtaggcg caaaccggat
     4621 gtttcgcttc gctatgcagc atggtggtat cagcccaacc ggtaacaccc tccttcggga
     4681 aaacggtagc aacaggctgg ccttcggctt tcagggcgtt ggcctgatag ggccaggcac
     4741 tggaagcaac cacaccctcg tttttgaaat cgctcatttg cacggtagtg tcatgccagt
     4801 agcgatggat caaactgtgt tgagcgcgca gcactttcag caccgcctgg tactgttctt
     4861 cggttagttg atacggatcg ctgatgccca actgcggctg agtggcttta acgaacaacg
     4921 cagcgtccgc aatgtagata gggccatcat aagcctgaac gcggccttta ttgctcttgc
     4981 cgtccggcag attttgctca acaaaaacca cttgccagct atccggcggc gtcgggaagg
     5041 ttttagtgtt gtacatcagc aggttcggcc cccattggta aggtgtgccg taaactttgc
     5101 cgccaacatt aaaccagtcg cctttaacca cgcgcggatc gagcgttttc cagttgggaa
     5161 tcaaggcggt attaatcggc tgcacgcgtt tgcccataat caaacgcagc gaggcatcgc
     5221 cggatgccgt aaccagatcg taaccccctt tggtcatcag actgaccatt tcatcggaag
     5281 tcgcggcggt tttcacattc accgcgcagc ctgtctcttt ttcaaattgc gttacccagt
     5341 cgtattgttt atcagtttgt ccgcgttcga tgtatcccgg ccaggcgata atatccagtc
     5401 gcccttccgg tttatctaaa ttggtaggtg gttcggcggc gtgagcggtc attattgtca
     5461 tggtgagcgc acacaggctg ctgcgggcaa atgtcttgct cataaggtct tactcctgtc
     5521 gtaattaatt gttcagccgc cgtgccgtaa aaatatctcc ttgtttaacc atagacggcg
     5581 gcttgcaggc acaccttcgc ccctgagaaa atttaatcag gttgtgagct accgcgcatt
     5641 atgccgtctg taaggttatc ttctggagta tagacaaggg gttaggaaat atgactgcta
     5701 ttcgaaattc atattaccca atctaagagg aattaaatta aacgctatta cagccgttct
     5761 tgaataagtt tgcctaattg ttttaccgcc tgttcttcac gttctcccca ctgccacgcg
     5821 gtattaaaac gaaaaaaacg tgaccagttt tcaccggtag aaaacatttt acccggcgca
     5881 atactgatat gatgcgtcag tgccgccagg cttaattcgc cggcatctaa cggctcgggg
     5941 agctccagcc agagaaaata accgctgtca ctatgatgaa ttttaacttc cgcaggcaga
     6001 taacgcagca gtgcctgcca ggcacgttgt ttacgttccg caagctggcg acgcaggcga
     6061 cgaagatggg cgtcgtatcg gcgcgtggaa aggtaatcca ccagcgcaag ctgcatcggt
     6121 gagctggtgg aaagcgtact catcaactgc aagcgttgaa ttttacgtgc atgttttccg
     6181 gcggcgaccc aaccaatacg aaaaccaggc accagacatt tcgaaaacga agagcaatgc
     6241 aaaacgccat cgtggcgatc ccacgctttc gcaggcagcg gtttttcccg tccaaaataa
     6301 agttcgctgt aaacgtcatc ttcaatcagc gttacgttgt actgattgag taacgccacc
     6361 agttgtgctt tttttgcggc gttaaggtaa atccgagtgg attctggcta ttagtcatca
     6421 gccagcacgc tttcaccgga tactcctgca acgccagttc caacgcctga agatctatcc
     6481 cttctttaac atccgtcgcc accgataacg ctttcagccg tagccgttcc agcgcctgca
     6541 acgcgccata gaaacaagga ttctctacta tcacccaatc gcccggttca gttaccgctt
     6601 gcaaactgag gtttaatgcc tctaacgccc cggcagtaat gacaatttca tcaggagaaa
     6661 tggtgatgcc ctgtaaggca tagcgacgag caatagcctg acgcagttct gcgtttcctg
     6721 gcggtaagtt ttcaatcacg ctcatcgccg tggcggtttt gcttacctgc gccagcgagc
     6781 ggtttagttg ttggaggggg aaaagtcgcg ggtcgggaaa ggccgaggca aacggaacga
     6841 ccgacggatc gcgactggcc tgcaacatat caaaaatata agtgttgata tcgactgctt
     6901 catctcgagt gactggaatg actggcgctt tcggcatttt tattgcctgt ggcgcaacgt
     6961 aataacccga ctgcggtcgt gcgataatat atccctgact ttcgagcaac tgataggcat
     7021 ggctgacagt cataaagctc atgcctgaaa gcgccacctg gtcacgcaac gaaggcaaac
     7081 gatcgccggg ttgccagata cccgacgcaa tctgctcgcg taattgttct gcaagctgct
     7141 ggtatttttt cattatcgaa tcgtaattat gtgcagatga ttcggcagtc tatatcagtt
     7201 gttaaaaatg acaacttttc gttaactgta attaaaccat caccagcgat aactctttgc
     7261 caagcgcctt tgccgcaagc tggacggcgt cgatttttgt cgcatggtgc aagttaaata
     7321 ggcgagtaat ctcctgttta ggtttgccaa ttcgcctggc taactcttgc tgagtgattt
     7381 ctgactgtaa aaaagcattt aacagcaata ctttagaggc gacgctcaaa ggtacttcaa
     7441 taaagtgatc atgactattt aatggcgaag gtaacgggat aagctcgtta tcttcaaaat
     7501 aaaaatcaaa tgcggtcagt aaagcatctt ttgccgcttc catcgcttca gcgacagttt
     7561 cgccctgcgt caacgcttca gggatatcca caaaagaaac catataaccg ccttccggcg
     7621 cgggtgtaag agtgacggga taacgcataa tttcgactgt ccctcgcatg tcattaacct
     7681 ttttaattgg cgattaactc aaaccgagtt gtttcaggat tgctttacgc aatggttctt
     7741 taatctcatc gcaggggtga cgcggcatga cactgcgcct cccatgaaac ctgagtttca
     7801 aatggttgct gccattcgct acatcgacgc cctgagattc gagccaacgt ctgaactcgc
     7861 tttgtttcac atcgcctccc tgccgctgag tatatcaaca aagtaaacat tagtgtttac
     7921 ctccgtgtgt aatttgctcg aaaatagcac taaatcgccg caacggcaga atgccatgca
     7981 aaatcaggaa gaaagactcc agaatttctt tgcgctaatt tgcaaaactg aataataacc
     8041 tcaagaaacg atcggcatca tagcggcaaa aggtaagaaa aaggtggcag gatgtttacg
     8101 aaggcgttat cggttgtctt attaacgtgt gcgctgttct caggacaact catggcaggg
     8161 cacaaaggac atgaatttgt gtgggtaaag aatgtggatc atcagctgcg tcatgaagcg
     8221 gacagcgatg aattgcgtgc tgtggcggaa gagtcggcgg aaggtttgcg cgagcatttt
     8281 tactggcaaa aatcgcgcaa accagaagcg ggacaacgtt gagtcatttg ttgccggatg
     8341 cggcgagacg ccttacccgg caggcaatat catgcagatt caatgagttg tgagtaacca
     8401 gaaaactggc gttttaccct tttcgcttag gcaatgtttt cagcagctca tccggactta
     8461 cggatgccac tacgcttccc ggtaacggca ttttcagtat gtgatttttg attttgccaa
     8521 tgacgtgcat ctcgcatggg cggcaatcaa actttagcgt taatacttca tcgccgttaa
     8581 ccagttgcat tggcgtcgcc ttccagcttt tgatattgcc tttcgcctgt ttcgggcaca
     8641 gattaaaggc aaagcgcaga caatgcttgg tgatcatcac cgggacttcg cccttctctt
     8701 catgtgcttc atacgccgcg tcaatcagct gcacaccata gcgatgataa aattcacgcg
     8761 ctttctggtt gtatacgttc gcgaagaaac tcagatgcgt ttgcggataa accggcgcag
     8821 gatcagcaac cggtttacgg ctgccgcgct ggtaactggc aagacgcgca gcatccagca
     8881 tgtcagcagc ttcacggcgg aactggttta acagactgtt tggtacaaac agcgcccccg
     8941 gcaaattaat ttgcacatcg cgggcgtaat agagggtttg ccccagtttt gccagaccat
     9001 ccttcagatt gttcattgct ttttcggcgt tattggcttc gtcgaactgc ccgtccagcg
     9061 tatgcgtgat gctgacaccc tcttcactgg tgagggtcag aatcagttgt tcctgccagc
     9121 cgcccagttc aatgtctacc gccacccgac gttcgctgga ggtttttgtc agtgcctgct
     9181 gccagttatg atcaagatta cggtttagtg ggtgatgtgg acgaattttg tgcaaatctg
     9241 ctggcatttc attgggccag acgcggtact gattttctcc ggttttctcg accgtattgg
     9301 cacgaaaacc gacgacttca cgtttaatca tcacgttcag gccatcacca tttgccagtg
     9361 gctcggtgac ggcaacatcg agatgatctt tcgccacttt caatacttcg cctaccggca
     9421 ggccgataaa tttcggcgaa tcgaacgcgc caatatcgcc tttacgggca ttcacaaaat
     9481 aatctgtgct accacggtgg aaagtctttt ccgtcgatgg aacaaagaaa tgttcagtac
     9541 gacctgatga agcgcgcgcc agatcgccac gttcttcaat aatggcatca agcatctggc
     9601 gataatgggc ggtgatattc ttcacgtagc tcatatcttt gtaacgccct tcaatcttga
     9661 aggagcgtac gccagcatca atcagcgcgc cgaggttggc agtctgatcg ttatctttca
     9721 tcgacagcag atgtttttca taggaaacca cccgcccctg atcgtctttc aatgtgtatg
     9781 gcaaacggca cgcctgcgag caatcaccac ggttggcgct acgccctgtt tgcgcatgag
     9841 aaatgtagca ctgacccgaa taggccacgc acagtgcccc atgaataaag aattcaatgg
     9901 tcgcgtccgt ggcctggtga atcgcgcgga tctgctcaag attcagctct cgcgccagca
     9961 caatctgcgt gaagccaaca tcagagagga acttcgcttt ttctactgta cgaatgtcgc
    10021 actgcgtact ggcgtgcagt tcaatcggcg gaatatcaag ttccagaatc cccatatcct
    10081 gaacaatcag cgcatcgaca ccggtctggt agaggtcggt aatcagccgt tgcgcgggtt
    10141 ccagctcatc atcatgcaaa atggtgttaa gcgtgacgaa aatttttgca ccataacgat
    10201 gggcaaacgg caccagctcg gcaatatctt tcaggctatt actggcatta tgacgggcac
    10261 caaaaccaga gccgccgata taaacagcat cggcaccgtg caaaatagct tcgcgggcaa
    10321 tggcggcatc gcgtgccggg cttaacagtt caagtcgatg agaagatacg gtcacattct
    10381 tatccagtag ctgaaaaatg gcggctattt tagccacaag cgcgacgttg cgaaatagtt
    10441 ttcccgctta actgcgcggg taatggatga gggaatgaaa atgtacggtt tgctccccac
    10501 cattacgata gatatgcgtg acgtctgcag caaatcgaac gccttcccca caattaaggg
    10561 tctgccattc cccatcaacg cacagatcaa gttgtccatc aatgacgacg acatgttcga
    10621 ttaccccctt ctcatggggc gtcgattcgc ttattgcacc agatgccatc tgaatcgaga
    10681 agtgttcgaa gcagagttgt ggatcataag gaaacagcga agtgatcacc atcgcctgct
    10741 gttgtggatc ataaactgaa ggcgtagcgg actgcggcgg agaaataaat gttgaaaatg
    10801 gtacgttcaa cccggtggca attttccata atgtcgcgac cgtcggactg gactcattac
    10861 gctcaatctg ccctaacatt gctttagaaa cgcccgtcgc ttctgccagt cgcgaaagac
    10921 tccagccgcg ctgctggcgt agttgtttaa gtgtggtgga taaaaagcga gcgagatttt
    10981 ccatgcgact cctccggcaa aacggaagtt tatcacttgt gcgttataac ggacaaatgc
    11041 tacggtgcct gtacgctata acgcacgagg tgactatgcg tctgttttct attcctccac
    11101 ccacgctact ggcggggttt ctggcggtat taattggcta cgccagttca gcggcaataa
    11161 tctggcaagc agcgattgtc gccggagcca ccactgcaca aatctctggc tggatgacgg
    11221 cgctggggct ggcaatgggc gtcagtacgc tgactctgac attatggtat cgcgtacctg
    11281 ttctcaccgc atggtcaacg cctggcgcgg ctttgttggt caccggattg cagggactaa
    11341 cacttaacga agccatcggc gtttttattg tcaccaacgc gttaatagtc ctctgcggca
    11401 taacgggact ctttgctcgt ctgatgcgca ttattccgca ctcgcttgcg gcggcaatgc
    11461 ttgccgggat tttattacgc tttggtttac aggcgtttgc cagtctggac ggtcaattta
    11521 cgttgtgtgg aagtatgttg ctggtatggc tggcaaccaa ggccgttgcg ccgcgctatg
    11581 cggtaattgc cgcgatgatt attgggatcg tgatcgttat cgcgcaaggt gacgttgtca
    11641 caactgatgt tgtctttaaa cccgttctcc ccacttatat tacccctgat ttttcgtttg
    11701 ctcacagcct gagcgttgca ctcccccttt ttctggtgac gatggcatcg caaaacgcac
    11761 cgggtatcgc agcaatgaaa gcagccgggt attcggctcc tgtttcgcca ttaattgtat
    11821 ttactggatt gctggcactg gttttttccc ctttcggcgt ttattccgtc ggtattgcgg
    11881 caatcaccgc ggctatttgc caaagcccgg aagcgcatcc ggataaagat caacgttggc
    11941 tggcagctgc cgttgcaggc attttttatt tgatcgcagg tctgtttggt agtgctatta
    12001 ccgggatgat ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt
    12061 taagtaccat tagcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgcgacgcgg
    12121 cggtggtggc atttctggta acggcaagtg gattgacgct ggtcgggatt ggttctgcgt
    12181 tctggggatt aattgccgga ggcgtttgtt acgtggtgtt gaatttaatc gctgacagaa
    12241 accgatattg acatcctcca cgccctgaag gacgaggatc cctgctacgt tcaggctgtc
    12301 gcctgaatca tttcggtggg ttcctgcttc aacgggcggc ctgactgcac catctctcca
    12361 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc
    12421 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcg
//
LOCUS       DAZFKM010000144        12167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFKM010000144 DAZFKM010000000
VERSION     DAZFKM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..211)
                     /locus_tag="ACHKAY_004210"
     CDS             complement(<1..211)
                     /locus_tag="ACHKAY_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702785.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQR"
     gene            complement(1031..1243)
                     /gene="yncH"
                     /locus_tag="ACHKAY_004211"
     CDS             complement(1031..1243)
                     /gene="yncH"
                     /locus_tag="ACHKAY_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO5702786.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1319..1936)
                     /gene="yncG"
                     /locus_tag="ACHKAY_004212"
     CDS             complement(1319..1936)
                     /gene="yncG"
                     /locus_tag="ACHKAY_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO5702787.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            2202..3701
                     /gene="ansP"
                     /locus_tag="ACHKAY_004213"
     CDS             2202..3701
                     /gene="ansP"
                     /locus_tag="ACHKAY_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO5702788.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3816..4877)
                     /gene="yncE"
                     /locus_tag="ACHKAY_004214"
     CDS             complement(3816..4877)
                     /gene="yncE"
                     /locus_tag="ACHKAY_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO5702789.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5119..7221
                     /gene="pqqU"
                     /locus_tag="ACHKAY_004215"
     CDS             5119..7221
                     /gene="pqqU"
                     /locus_tag="ACHKAY_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO5702790.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7257..7922)
                     /gene="mcbR"
                     /locus_tag="ACHKAY_004216"
     CDS             complement(7257..7922)
                     /gene="mcbR"
                     /locus_tag="ACHKAY_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO5702791.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(8120..9157)
                     /gene="curA"
                     /locus_tag="ACHKAY_004217"
     CDS             complement(8120..9157)
                     /gene="curA"
                     /locus_tag="ACHKAY_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO5702792.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVLVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            9338..9856
                     /gene="mddA"
                     /locus_tag="ACHKAY_004218"
     CDS             9338..9856
                     /gene="mddA"
                     /locus_tag="ACHKAY_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO5702793.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            9853..10302
                     /gene="ydcZ"
                     /locus_tag="ACHKAY_004219"
     CDS             9853..10302
                     /gene="ydcZ"
                     /locus_tag="ACHKAY_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO5702794.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(10303..10536)
                     /gene="ydcY"
                     /locus_tag="ACHKAY_004220"
     CDS             complement(10303..10536)
                     /gene="ydcY"
                     /locus_tag="ACHKAY_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO5702795.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(10622..10795)
                     /gene="ortT"
                     /locus_tag="ACHKAY_004221"
     CDS             complement(10622..10795)
                     /gene="ortT"
                     /locus_tag="ACHKAY_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO5702796.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            10990..11085
                     /gene="yncL"
                     /locus_tag="ACHKAY_004222"
     CDS             10990..11085
                     /gene="yncL"
                     /locus_tag="ACHKAY_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO5702797.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(11487..>12167)
                     /locus_tag="ACHKAY_004223"
     CDS             complement(11487..>12167)
                     /locus_tag="ACHKAY_004223"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO5702798.1"
                     /translation="KERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTC
                     HFSGNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPF
                     ITKPENPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIR
                     FRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFN
                     LSEVFRGL"
BASE COUNT         3122 a         2882 c         2813 g         3350 t
ORIGIN      
        1 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc atccggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
      241 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
      301 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
      361 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
      421 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
      481 tcaaaccaat attcataatg tctttttatt cacaacaata aataattcaa taacaatcat
      541 tgaaatgaat gatggtttgt ataaggaata tacgctaccg ccccaccctg ataacatgat
      601 ctgcacattg ccatcttaac gtcgttaaac actagcttaa tagtggatat ttcaaattag
      661 gaatttatta aattcacctg atgaatataa taagcaaata gcatagaaaa taatgacaac
      721 tcccccgagg ggaccatttc caaagcactg caccaccacc tggtgccgga tgatcttgct
      781 tcagtggtaa gagtacctga tgcattgttc ccaaaatggt gttttcccct gcccctccaa
      841 taatgtgtag ctctcacttg ataatctatt gattaggtta tattaagaat caacatttaa
      901 aatacatcca ttttcttgaa taaatcaaca tgtttattaa tcaattgctg ttttagctct
      961 gactcatcaa gctctccata tttgtcctac agataattgc gaataaatac agagaacgcc
     1021 atttatcctt ttagcatacc tgccggatat ttttatttgc attaatatta attgcatctc
     1081 caactccgag catatccatc attaaaaact ttttgagatt atagaatacc tgtgataaca
     1141 agtgaaacat ctgcacaatg tgaatagtta gtttcttttc agaggaaatg aaatagacaa
     1201 gctgaatgaa ggggagagtg atataaatca aaaaacataa catgaattta attaattaca
     1261 cccatcattg tggataaagt tactgaataa taaataacat aatcttctgg tatcccactc
     1321 aaataatttc attccttttt aatacttctt gtaatttcgg taactgacat acagcatcag
     1381 cgatcgcgct gatattctgc gcattatcct gaaaccattc atgccccggc ccccatgtgc
     1441 gcatggtgca gagataacag tccaccagcg ttagttgctc accaaaggca taaggttcag
     1501 cggtcaattg tgaattcagc cagatataaa gtgatttgcg atattcaatt acattcttct
     1561 ttaactgttc aggtgcgtca gatgcccaac gctcagggta atcggcgaaa gtgaatgttg
     1621 gatagacatt agcaaccagc cagaccaata gccgttgaaa ctgctgacgt tcggcgcgcc
     1681 caaccggtgg cgcaaggtcc gggcgacgat cgagaaccat aaatgcaatc gccgctgtct
     1741 ccgtcataat ctcgtcattt tccagcgcca gtgtcggaac ctgacacaac gggttcaggg
     1801 ttttcaataa ctcgcgtgaa gctccctcgt ggtcaaaacc actgacatca acgaactgat
     1861 aaggaatatc agccagtgtt agcatcagct cactgattgt agagccccat ccgggtacgc
     1921 catacacctt aatcatatgc cctcacaatc ttttaaagat taagtgtagg acagtacaca
     1981 ggttaaccag caaaaagatt tgtcattttt tcgtcagttg tattattccc cttcaggatt
     2041 gtaaggggat gttttgcatc gtttatcgat aacgggtagg atgctgcgcc gatagtttta
     2101 tacccataaa gaataatggt gataactatc atcgccagga tgaataaaca ttgttcatgg
     2161 caacttatat gactttttca ttaaagcaat cagggagagc aatgagtaaa cacgacaccg
     2221 acacttcaga tcaacacgcc gcgaaacgcc gctggcttaa tgcccacgaa gaggggtatc
     2281 acaaagcgat gggcaatcgc caggtacaga tgatcgccat tggcggcgcg attggcaccg
     2341 gcttgttttt aggtgcagga gcccgactgc aaatggcggg tccagcattg gcactggttt
     2401 atttaatttg tggcttgttt tcgtttttta ttctgcgtgc attgggtgag ctggtgctac
     2461 accgcccttc cagtggcagt tttgtctctt atgcccgtga gtttttgggt gagaaagccg
     2521 cttatgttgc tggctggatg tacttcatca actgggcgat gacggggatt gttgatatta
     2581 ccgccgtcgc tctgtatatg cattactggg gtgcgtttgg cggcgtgccg cagtgggtct
     2641 ttgcgctcgc tgcacttacc atcgttggca ccatgaatat gatcggtgtg aaatggtttg
     2701 cggagatgga gttctggttt gcgcttatta aagtgctcgc cattgtgacc tttttggtcg
     2761 tgggtacagt gttcctcggt agtggtcagc cgctggatgg caacaccact ggctttcatt
     2821 taattaccga taatggcggc ttcttccccc acggtttgct gcctgctctg gtgttgattc
     2881 agggcgtagt gtttgctttt gcctccattg aaatggtggg tacagctgcc ggagaatgta
     2941 aagatccgca gaccatggtg cctaaagcga ttaacagcgt gatttggcgt attggcctgt
     3001 tttacgtcgg ctccgtggtg ttgctggtta tgctattgcc gtggagcgcg tatcaggcgg
     3061 ggcaaagtcc gttcgtgacg tttttctcta aactgggtgt gccatatatc ggcagcatta
     3121 tgaacattgt ggtgctgacc gctgccctct ccagcctgaa ctcaggtctg tactgcaccg
     3181 gacgtattct gcgctcaatg gcgatgggcg gttccgcacc gagttttatg gcgaaaatga
     3241 gtcgtcagca tgtgccgtat gccgggattc tggcgacact agttgtgtat gtcgtcggcg
     3301 tattcctcaa ctatctggtg ccgtcgcgcg tatttgagat tgtgttgaac ttcgcgtcgc
     3361 tgggaatcat cgcttcatgg gcgtttatca tcgtgtgcca gatgcgcctg cgtaaagcaa
     3421 ttaaacaagg caaagcagcg gatgtcagtt ttaaactgcc tggcgcgccc ttcacttcct
     3481 ggctgacatt actgttttta ctgagtgtcc tggtgctgat ggcgttcgat tacccgaacg
     3541 ggacttatac tatcgcggcg ctgccgatta tcggtattct gctggttata ggctggtttg
     3601 gtgtgcgcaa acgcgttgct gaaattcaca gcactgcgcc agtcgtcgag gaagatgaag
     3661 aaaaacagga aattgtgttt aagcctgaaa cggcgagcta attgcaaaat tgcccggcta
     3721 cgtgccgggc aaatttagat tactgacgaa ggtgtgccgc atgcggcgtg ataaaagtca
     3781 ggcaagtctc tccgttttca ccctcgccac atctattaca gcgcaatacg aatcacatcg
     3841 tccggctggg tagcttcctg ctgtttagtg gatttttgtt tcacactgac atacagcgtt
     3901 ttgccatcgg cagacagcgc caggctgttt ggatgagtcg gcgtatcgaa cgttttcacc
     3961 actttatagc ttttcgcgtc aatcacactg actttacctg cctgacgatg cgttacgtag
     4021 gcttcattac gcgctgggtt aaacagcaca gccagtgatt ctggtgccgc aaccttcgcc
     4081 agaatattgc cattacgggt atcgaccact aacacttctg cggctttaga atcggtgata
     4141 aatgcacgct gcctggcggt atcaaggcta atgttgataa agaagtgctc tttgccgtca
     4201 tccagcagct ttttacggct gaggatttta ttgtcggcgg tgtcgatggt aatcaattcg
     4261 ccgtcagcgt tagtggtgta aagacgtttg cctttgctat ccagcgccag accggtactc
     4321 attttaccgg tgttctggat ggcggttttc agtttgatat tctcgccatc aacgacccaa
     4381 atcacgctct ctttaccaat accactgatg taaacggtgt tcgtggcatc atcagctacc
     4441 agctcacgcg gttgcagcgg gcgcacctct tccgtgcgct tacgatcatc cagcaccaga
     4501 cggcctttca cctcgcccgt tttggcatct atcgccgtga ccgcgctgtt tacggtgtta
     4561 ccaaaccaca acgtctgagt cgtgttattg atggtggcac caaacggctt gagatcgtta
     4621 tggatcgcct gcgtcacttc cagagtgacc ggatcaagac gataaaccac gccaccttta
     4681 tccagtttgc ggctttgcga agtggcgagc cacagtgcgt tttcttgctg gctataagcc
     4741 atttcgtagg cacctttacc taccgcttta cgcagcattt cttctgcggc ctgcgtactg
     4801 aatgatgaag caacaagcaa tgaacctaac agtaatgaac cacgcaggcg cgatgaaaac
     4861 agatgacgta aatgcatgac gactcccttt gatgaaccga tatttttgct gcttcacaat
     4921 gcttcgctaa gcgtaatcat cgttacgctc ttgttataaa tgggaatggt aatcattatt
     4981 ttctcaaatg tggagcattt cttgccgtta cgactacaat tttctcaatt acttaacgta
     5041 tcgtgccgtt tcgttttcca taaatttaca atttcattac cataacggcg ttttcccatt
     5101 cttaacctgg cttttttcat gaagattttt tccgtccgac agaccgtttt gcccgcactg
     5161 cttgtccttt cccccgttgt ttttgccgct gatgaacaga ctatgattgt cagtgccgca
     5221 ccgcaggtgg tttcagaact ggatacccca gcagcagtaa gcgtggtgga tggcgaggag
     5281 atgcgcctgg caacaccgcg cattaacttg tccgaatcac tgaccggcgt gcctggtttg
     5341 caggtacaaa accggcagaa ctatgcgcaa gatttacagc tgtcgattcg cggatttggc
     5401 tcccgctcca cttacggtat tcgcggtatt cgcctgtatg tggacggtat tcccgccacc
     5461 atgcccgacg ggcaagggca aacatccaac atcgatttaa gcagtgtgca aaatgtggaa
     5521 gtgctgcgtg gccccttctc tgccctgtat ggcaacgcgt ctggtggggt aatgaatgtc
     5581 accacccaga ccggacaaca gccaccaacc attgaagcca gtagttacta cggcagtttt
     5641 ggcagctggc gctatgggct gaaagcaacg ggcgcaacgg gagacggcac acagcctggc
     5701 gatgtcgatt acaccgtctc aaccacgcgt tttacgaccc acggctatcg tgaccatagt
     5761 ggcgcacaga aaaatttagc taatgccaaa ctgggcgtac gcattgatga agccagcaaa
     5821 ttaagtctga ttttcaatag tgtggatatc aaagcagatg acccaggtgg gctaaccaaa
     5881 gcagaatgga aggctaatcc acaacaagcg cctcgtgcag aacagtacga cacgcgaaaa
     5941 accatcaagc aaactcaggc tgggttgcgc tatgagcgta gcctgagcgc gcaagatgat
     6001 atgagtgtga tgatgtatgc cggagagcga gaaacgaccc agtaccagtc aatcccgatg
     6061 gccccccaac ttaacccgtc acatgcgggc ggcgtgatta ccctgcaacg ccattaccag
     6121 gggatagaca gccgctggac acaccgtggt gaactgggcg ttccggtcac gttcactacc
     6181 ggcctgaact acgaaaacat gagtgaaaac cgcaagggct acaataactt ccgcctgaat
     6241 agcggcatgc cggagtacgg gcaaaaaggt gagttgcgtc gcgacgaacg caatctgatg
     6301 tggaacatcg atccctattt acagacgcag tggcagctga gcgaaaaact gtcgctggat
     6361 gctggcgtgc gctacagctc cgtgtggttt gattccaacg accattacgt tactccgggt
     6421 aacggcgatg acagcggtga tgccagttat cataaatggc tacctgccgg ttcgttaaaa
     6481 tatgcaatga ccgatgcctg gaatatctat ctggcagccg ggcgaggttt tgaaacgccg
     6541 acgattaatg agctgtctta tcgtgctgat gggcaaagcg gtatgaactt tggtttaaaa
     6601 ccatccacca acgatacaat tgagatcggc agtaaaacgc gtattggtga tgggctgctt
     6661 agtctcgcat tgtttcagac cgacactgat gatgaaattg ttgtcgatag cagtagcggt
     6721 gggcgtacga cttacaaaaa tgccggaaag acccgtcgtc aaggcgctga actggcatgg
     6781 gatcaacgtt tcgcaggaga ttttcgcgta aacgcgtcct ggacctggct tgatgcgacc
     6841 tatcgcagca atgtttgcaa tgaacaggat tgtaacggta atcggatgcc agggatcgcc
     6901 cgtaatatgg gctttgcgtc gataggttat gtaccggaag atggttggta tgcaggcacg
     6961 gaagcgcgtt atatgggcga tattatggca gatgatgaaa atacggcaaa agcgccgtct
     7021 tatactctcg tcggcttatt caccgggtat aaatacaatt accacaattt aactgtggat
     7081 ttatttggtc gtgtcgataa tttattcgat aaagaatacg ttggttctgt cattgtcaat
     7141 gagtcaaacg ggcgatatta cgaaccttcg cccggacgaa attatggtgt cggcatgaat
     7201 attgcgtgga gatttgagta attaccttga tgcccggtat ttgccgggca tttactttaa
     7261 cgattgtatt gctggtataa aatagcaata ttttgttgca taatttcagc aagacaatgt
     7321 ctgcatccct ctttgtcttt tgcttttaac gcagcgagta acagatgata gttttctata
     7381 tgctcccgta attccgctgg attaatcgct tcatagagat aatgtaaacc aggccccatc
     7441 ctgacccaca gttgctcaat catctcacac aggatgggca tatttgagcg atgataaatc
     7501 gctaagcgaa atagcctgtt tacattaatg atttgttcca tgtcgccctt ttcttgcgcc
     7561 tgctgtaatt tctcgagcaa ttcctggagt tccgccaggt cttgcggggt gaggttttca
     7621 acagccagag caactgccat taattccagt tcgtagcgga tccgattgat ttcgtctaat
     7681 tgacgtttcc tcacttccgg aactgtaaat gcttgtgcag gtgcgacaga aagcgcattc
     7741 accgaaacca gacgtaataa tgcttcacgc acaggtgtaa tactcatacc taattgctcc
     7801 gccagatttt tagtaatcag gcgtgcgcca ggttttaatg cgccaatact aagctgatgt
     7861 ttcaggtcgt tctcaacctg cagagtcaaa ctgacatgtt tcatttttcc cattccaggc
     7921 attttaattc cttcgcgtgt tttctgtcca accctgcctg cacggcagaa ggagttcacc
     7981 tggtgtatta aagtgaataa tcataaacca cccacctttg ttgttattag cagcagaaca
     8041 atgacgtggt tagttaatta aagctgaaaa taagcttttt gcctggattt ttatggcggc
     8101 ttacgccgcc gttgaaactt taatcatcac ccgcgacgcg gatcaccact ttgccgaagt
     8161 ttttaccctt cagcaggccg ataaacgtct gtggcgcatt ctctaaacca tcagtaatat
     8221 cttcgtggta gtggatttta tcctctttca cccattgccc catctccttc tgaaactcat
     8281 ggatgcggtg accataatcc tgagcgataa taaacccttg caagcgaata cgttttttca
     8341 gcaccgttgc catcaacagg ggtaaacgat ccggaccggg tggtagctct gtagcgttat
     8401 agctgctcac taagccacaa acgggaatgc gcgcagatgt attaagtaat ggtagcactg
     8461 catcaaatac cttaccgccc acgttttcat aatagatatc aatacctttt gggcacgctt
     8521 tcaccagttg ttcggcaaaa tcatccgcgt ggtgatcaag acaaacatcg aagcccagca
     8581 cctcgatagc atggcggcat ttttccgcgc caccggcaac ccccaccacc ctgcaacctt
     8641 taagtttgcc gatttgcccc accgttgctc ccacaggacc tgtcgccgca gctaccacca
     8701 acgtttcgcc ctctttaggc tgaccgatat ccagcagccc catataagcg gtaaagcctg
     8761 gcatccccag cacacccagc gaccacgatg gattttgcgg atgatcgcca agtttcacca
     8821 gatcatcacc actggatatg tcatagtcct gccatccact gtagcccagc acccagtcgc
     8881 caggctgata atcaggatga ttcgactcca caacacggct caccgtgccg ccgaccaaca
     8941 cgccgccaat atcaacaggc ggtgaataag atggctcatc gctcatacgt ccacgcatat
     9001 acgggtccag ggacaaataa actgtgcgca gtaacacctg tccttcaccc ggtgtggcga
     9061 catcatcttc ttcaagacgg aaattctccg gaaccggtgc gccatgtgga cgcgaggcca
     9121 gaacccaacg tcgattacgc tgcttttgtt gcctcataat gctctcctgt gcttttggca
     9181 tgaatccatc aatagtagca ggctcacggg attggttttg tttgcagccg tcacatcccc
     9241 tctgcaacac gcaataattt catcgaggaa tccgttgctt cgggctgttt tcgatagtat
     9301 tgataacatc atcatcatta ttttatcgag gccagacatg tccatccgtt ttgcccgcaa
     9361 agccgattgt gctgccattg cggaaattta taaccacgcc gtgttgtata cggcggctat
     9421 ctggaatgac caaacggttg atgcagataa ccgcattgcc tggtttgaag cgcggaccat
     9481 agcaggttat ccggtgctgg tgagtgaaga agatggcgta gtgacgggat atgcctcgtt
     9541 tggcgactgg cgtagtttcg atggttttcg ccataccgtg gaacattcgg tttatgtcca
     9601 tcccgatcat cagggcaaag gtctggggcg taaattgtta agccgattga ttgatgaagc
     9661 gcgggattgc gggaagcatg tcatggtcgc cgggatcgaa tcgcaaaatc aggcctcgct
     9721 gcatctgcac cagtcgctgg gatttgtcgt caccgcacaa atgccgcagg tgggcactaa
     9781 atttgggcgc tggctggatc tgacatttat gcagttgcaa ctcgacgaac gcactgaacc
     9841 ggacgcgatt ggatgaatca gtcgctcacc cttgcttttt tgattgccgc cggaattggt
     9901 ctggtagtcc agaacacgct gatggtacgc atcacccaga cctcctctac cattctcatc
     9961 gccatgttgc tgaactcact ggtggggatt gtgctctttg tttctatttt atggtttaaa
    10021 caaggcatgg cggggtttgg cgaactggtg tccagcgtgc gctggtggac acttataccc
    10081 ggcttgctgg gatcgttttt tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg
    10141 gcgacaacta ttgcagtact ggtcgccagc cagctaattg gcggtctgat gctggatata
    10201 ttccgcagtc atggcgtacc actacgggcg ttgttcggcc caatttgtgg cgcaatttta
    10261 cttgtggttg gcgcctggtt ggtagccaga cgctcatttt aatcagagga tggtgccgcc
    10321 tttggttaac tgttcgtggc gcgcttccat atcttcttta tgcttgcgac cgtgatgagc
    10381 aatagcggtg cgcaaacgct gctgctgggt gtagcgatct tcccgactta actccgcgtc
    10441 atcgctcagg gcgattaata attcgttcat atgggcaatg acgctctctt caatcgcggc
    10501 atccacgcgc gcgatgactt catccagatg cgacatactt tctccttaac cacttgtgaa
    10561 atttatcgat tcatccaaat gatataagtc tgcgtggtgc cgtcaagcca gtgagtaaga
    10621 tttaaaacag tgaaaacaac agcgccaccg ggaaactcat cggccatgtt gcccccacca
    10681 gaaaggcgct taagaaacgg atgcgtttct tatcgtgaga aagaaaccag gtgataagaa
    10741 atgcgattgc tgccataacc gcataaaaaa caagcatatg ttgatagaga gacatgtacg
    10801 ggcgtccagc cagatgaata aaacgcgcgc aaatatgcgt cattttttga cgtgcatcaa
    10861 gttttttcac cagtttgaga aaaaatcatc aaacagcaac ttgcccttat ccatcttcag
    10921 aatgagtatt attgcggatt ttcttaaccc gtactataca cataggggca atgataaaag
    10981 gtggcaaaaa tgaatgtttc cagtagaact gtagtactga taaatgtctt tgctgctgtt
    11041 ggtttgttta ctcttatctc tatgagattt ggctggttta tttgatgtag tgattttgca
    11101 agcagcacgt actgtcagag aaatcaactc tctgacagta cgcaagatct tctcatactc
    11161 tccaggttta caacattatc cttaatattt acttcttttt gctggcatct acattgaaat
    11221 gagtagaaaa catcccccta ctgtcgtcta tacaaacagc ttactgattc aaacggaatc
    11281 ttgttttcac atttttctat catgaatact ttatacgcgc gggagtctat aaatgcttta
    11341 tactttcaag gaataacgta aatataaaaa gatggcgtaa agtatatgca tggcaggatg
    11401 tttgcaacct ccttgatgac gctgccccat tgaaattgca aaccccgccc tggttatctt
    11461 gcccatttat catgcaaaac agataattat aggcctctga atacctcaga aagattgaat
    11521 tttttatagt ccctctcttg aatatttcca tctgatatca atcgattcat ctcttcaaca
    11581 aaataattta tatcatcaca cttgttatta tccggaagat ctgagtagtt ataattccac
    11641 cattttattc tcaacagttc atcaataaca tttgaatcaa acctaaatct gattattttt
    11701 gcaggaactc ctgcaactat ggcatatggg ggcacatctt tggtaacaac ggaattggca
    11761 gcaataacag caccatcacc tattgcgatg ccacctttca atacaacatc atttcctatc
    11821 cagacatcat ttcctataat cggattttca ggtttggtaa tgaatggttt caatgtccag
    11881 ctacgttcaa attcactcat agcaaactta tcaaattcac cgttataagt catcatgtga
    11941 gtagtaaaac gattcaaagg atgatttcca cccattattt ttacatttga tgcaatacta
    12001 caaaatcgac ctatacgtat attccctgaa aaatggcagg tcggaaatga aaaagcgccg
    12061 acactggaga aatttttcac aggcattgtt gaatattctt cagcaataac attatcttca
    12121 attgttatct tgtcaccttt tgagaaaaga aataaattcc tttcttt
//
LOCUS       DAZFKM010000145        12060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFKM010000145 DAZFKM010000000
VERSION     DAZFKM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..625
                     /gene="yebB"
                     /locus_tag="ACHKAY_004224"
     CDS             23..625
                     /gene="yebB"
                     /locus_tag="ACHKAY_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO5702799.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(627..1148)
                     /gene="ruvC"
                     /locus_tag="ACHKAY_004225"
     CDS             complement(627..1148)
                     /gene="ruvC"
                     /locus_tag="ACHKAY_004225"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO5702800.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1183..1923)
                     /gene="yebC"
                     /locus_tag="ACHKAY_004226"
     CDS             complement(1183..1923)
                     /gene="yebC"
                     /locus_tag="ACHKAY_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5702801.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1952..2404)
                     /gene="nudB"
                     /locus_tag="ACHKAY_004227"
     CDS             complement(1952..2404)
                     /gene="nudB"
                     /locus_tag="ACHKAY_004227"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO5702802.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2522..4294)
                     /gene="aspS"
                     /locus_tag="ACHKAY_004228"
     CDS             complement(2522..4294)
                     /gene="aspS"
                     /locus_tag="ACHKAY_004228"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO5702803.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4604..5170
                     /gene="yecD"
                     /locus_tag="ACHKAY_004229"
     CDS             4604..5170
                     /gene="yecD"
                     /locus_tag="ACHKAY_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO5702804.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5167..5985
                     /gene="yecE"
                     /locus_tag="ACHKAY_004230"
     CDS             5167..5985
                     /gene="yecE"
                     /locus_tag="ACHKAY_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO5702805.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6038..6433
                     /gene="yecN"
                     /locus_tag="ACHKAY_004231"
     CDS             6038..6433
                     /gene="yecN"
                     /locus_tag="ACHKAY_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO5702806.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6474..7217
                     /gene="cmoA"
                     /locus_tag="ACHKAY_004232"
     CDS             6474..7217
                     /gene="cmoA"
                     /locus_tag="ACHKAY_004232"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO5702807.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7214..8185
                     /gene="cmoB"
                     /locus_tag="ACHKAY_004233"
     CDS             7214..8185
                     /gene="cmoB"
                     /locus_tag="ACHKAY_004233"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO5702808.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8350..10779)
                     /gene="torZ"
                     /locus_tag="ACHKAY_004234"
     CDS             complement(8350..10779)
                     /gene="torZ"
                     /locus_tag="ACHKAY_004234"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO5702809.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10804..11904)
                     /gene="torY"
                     /locus_tag="ACHKAY_004235"
     CDS             complement(10804..11904)
                     /gene="torY"
                     /locus_tag="ACHKAY_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO5702810.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2767 a         3212 c         2920 g         3161 t
ORIGIN      
        1 tctatttcaa tggaatgaca acgtgaatat taattaccct gctgaatatg aaattggtga
       61 tatcgtcttt acatgtatag gtgctgcctt atttggtcaa atatcagctg catcaaactg
      121 ctggagtaat cacgtcggga tcattatcgg tcataacggt gaagactttc tggttgcaga
      181 aagccgtgtt cccctctcaa ccatcactac gctatcccgt tttattaaac gctctgctaa
      241 tcaacgctat gctataaagc gattagacgc cgggctgaca gaacaacaga aacaacgaat
      301 tgttgaacag gttccctccc ggctacgcaa actttaccac acgggtttta aatacgaatc
      361 ttcccgccag ttctgttcaa aatttgtctt tgatatttat aaagaggcgc tatgtattcc
      421 ggtgggtgaa atagagacgt ttggacaact gttaaatagc aatccgaatg caaaactcac
      481 tttctggaaa ttctggttct taggttctat tccgtgggag cgtaaaaccg tcacgccagc
      541 cagtttgtgg catcatccgg gtttggtgtt gattcacgcg gagggagttg aaacgcctca
      601 gcctgaactg accgaggcgg tataacttaa cgcagtcgcc ctctcgccag gttcagccgc
      661 gattcgctca tctgcattgc attctggctg acgtgacaat gggtaatcgc gatagctaac
      721 gcatcagcgg catccgcttg tggattagcg ggcagtttca gcaaggtgcg gaccatatgc
      781 tgcacctggc ttttctcggc gctaccaata cctaccaccg tttgctttac ctgacgtgcc
      841 gcgtattcaa ataccggcaa ttcctgattc accgccgcca caatcgccac gccgcgcgcc
      901 tggcccagtt tcagggctga gtcagcgttc ttcgccataa agacttgttc aatggcgaaa
      961 taatcaggct ggaactgggt gatgatttcc gtcacgcccg catagatgag cttcagacga
     1021 gacggtaaat catccacttt ggtgcgtatg catccactac ccaggtagga cagttgcctg
     1081 cctacctggc ggatgacgcc atagccggtc acgcgcgaac ccggatcaat gccgagaata
     1141 atagccatca cgcgtctccg ttttgctgtt tagcaggcct catcagagag tcgctgcaac
     1201 ctcatcagag atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat
     1261 atcgatcaga cgcatcagtt tcggtgcggt ttctgcatcc atatcagctt tggtggacgg
     1321 gatcatggaa acttccgcgc tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac
     1381 tttacccatt tcttcccatg cggtgtagac atcaatcgcg ccgtcatcat aggtcacaac
     1441 gtcttcagca cctgcttcca aagctgcttc catgatggtg tcttcatcgc ctttctcgaa
     1501 ggagatcacg ccttttttgc tgaacagata ggctacggaa ccatcagtac cgaggttacc
     1561 gccacatttg ctgaatgcat gacgcacttc agcaacggta cggttgcggt tgtcagacag
     1621 acattcaatc atgattgccg tgccgccagg accgtaacct tcgtagatga tggtttccat
     1681 gtttgcatca tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg tgtcgcgggt
     1741 catgttgtta gacagtgctt tatcaattgc tgcacgcaga cgcgggttag cgtccggatc
     1801 gccaccgccc agcttagccg cggttaccag ctcacgaatg attttagtga agattttacc
     1861 gcgctttgca tcctgcgcag ctttacgatg tctggtgttg gcccatttac tatgacctgc
     1921 cataaaaata tctccagata gccctgcctg ttcaggcagc gttaatgaca aactgttcaa
     1981 tcgcctgccg gttgctccag gacttagtga gcgccgccgc agcaggcgca tcaagccact
     2041 tgtaagccag atgttcagtg aaaacgatct gccgctcgtg cggaagcgca agacagaacc
     2101 atgattccgt attacgcgtc acgcccggcg catagcgatg acgtaaatgt gaaaaaattt
     2161 caaactctac cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca acatcaatgg
     2221 tgacctcttc ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc
     2281 tgccggttac cgactgccag aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt
     2341 tcgtatcttg tgcatagatg accactaaga tcgaaacggg acgcttatac actttatcct
     2401 tcacgctgcc tcttcgttga ctgccttcgc tcatcccatt cacatagtta tctatgctca
     2461 tgggagttca ctcagttgcc gccttgatgc aacgcgaatg atttcgtgta tttgagtcat
     2521 atcagttatt ctcagccttc ttcacaacct gaatgctcag ctcagccagt gcagtcgggt
     2581 tagcaaagct cggtgcttca gtcatcagac acgctgccgc cgtggttttc gggaaggcga
     2641 taacgtcacg gatattgtcg gtgccagtca gcagcatggt cagacggtca agaccgaatg
     2701 ccagacctgc gtgcggcgga gtaccgtatt tcagggcgtc gagcaggaag ccgaatttct
     2761 cgcgctgttc ctcttcgttg atacccagaa taccaaacac cgtctgctgc atatcaccat
     2821 tatggatacg tactgaacca ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt
     2881 tcgccaccgc attttccggt gcagctttca gttctgcagc agtcatgtct ttcggtgagg
     2941 tgaacggatg gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt
     3001 cgataaccca cagcggtgcc catttgcttt cgtcggtcag accaaggtct ttacccactt
     3061 tcaggcgcag tgcgcccatc gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa
     3121 tcatatcgcc atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt tctgcattaa
     3181 ggaacttcgc taccgggctg ttgatacctt ccagaccttt cgcgcgttcg ttaactttga
     3241 tgtaagccag acctttcgcg ccgtagattt taacgaagtt accgtattcg tcgatctgct
     3301 tacgggtcag cgatgcgcca cccggaacac gtagagcggc aacacggcct ttcggatcgt
     3361 tcgccggccc cgcaaatact gcaaactcaa cagatttcag cagatcggca acgtcggtca
     3421 gttccatcgg gttacgcaga tccggtttat cagaaccata acggcgttct gcttccgcaa
     3481 aggtcattac cgggaaatcg cccagatcca cgcccttcac ttccagccac aaatgacgca
     3541 ccagcgcttc catcacttca cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga
     3601 tctgagtaaa ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag catttaacga
     3661 tctgatagta acggtcaaaa ccggacatca tcagcagctg tttgaacaac tgtggggatt
     3721 gcggcagtgc gtagaattta cctttgtgca cacgagaagg caccaggtag tcacgcgcgc
     3781 cttccggcgt ggctttggtc agcatcggag tttcgatgtc gaggaagccg tggtcatcca
     3841 taaaacggcg caccaggctg gtgattttag cgcgggtttt caggcgctga gccatttccg
     3901 gacgacgcag gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg acgtggttag
     3961 agtcaagcgg cagaacatct gcgcggttga tgatagtcag cgaggacgcc agcacttcga
     4021 tttcgccggt cgccatatcg cggttaatat ttttttcgtc acgcgcacgt acggtgcccg
     4081 tgacctgaat gcagaactca ttacgcagtt cagaggccag ctttaacgcg tccgcacgat
     4141 ccggatcgaa aaatacctgc acgatacctt cgcggtcgcg catatcgatg aagatcaggc
     4201 taccaagatc acgacgacgg ttgacccaac cacacagagt cacctgctgc cccacgtggg
     4261 acaaacggag ctgtccacaa tattctgtac gcatgagata tcccttaact tagctgccgg
     4321 cggatgcccc ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa
     4381 tgaaaaaagg cggctattat actggaaatt ctgccgcacc gtaagagcct ggcccgcgct
     4441 ggaacgcctc gttaccactt tatatcgggc ctgaaatcag actctacgcc agtttgctat
     4501 aaaggtgttg cccgaactca taaaaattaa caaaatttgt cgttccgcca tcggctaatc
     4561 gcattaaggt gtgaggcacg attttgtttt ttcaggagtc attatgcttg aacttaatgc
     4621 taaaaccact gcgctggtgg tgatcgattt acaagaaggc atcttacctt ttgccggtgg
     4681 tccacatact gccgatgagg tagttaatcg cgccgggaag ctggcggcga aatttcgcgc
     4741 cagcggtcag cccgtgtttc tggtgcgcgt tggctggtct gccgattacg ccgaagcatt
     4801 aaaacagccg gttgatgccc cctcccccgc taaagtgttg cccgaaaact ggtggcaaca
     4861 tccagctgca ttaggtgcaa ctgacagtga tatcgaaatc atcaaacgtc agtggggtgc
     4921 gttttacggt acggatctgg agttgcaact gcgccgccga ggtatcgata caatagtgtt
     4981 atgtgggatc tcgaccaata tcggtgttga atccaccgcc cgcaatgcct gggaacttgg
     5041 ctttaatctg gtgattgccg aagacgcctg tagtgccgct agcgccgagc agcacaataa
     5101 cagcatcaac catatctacc cacgcatcgc ccgtgtgcgt agcgtggaag agatccttca
     5161 cgcgttatga tttacatcgg tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg
     5221 atcaccagcc ttgaagagta tgcccgccac tttaactgcg tggagggcaa caccacgctt
     5281 tacgccctgc cgaaacccga ggttgtcctg cgctggcgtg aacagaccac agatgacttc
     5341 cgcttctgtt ttaagtttcc ggcgaccatt tcgcatcagg cagcattacg acattgcgat
     5401 gatttagtga ctgaattttt gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac
     5461 tggctgcaac tgcctgccac attcggccca cgggagctgc ctgcgctttg gcattttctc
     5521 gattctctcc ctggcgaatt taattatggt gtggaagtcc gccatccaca gtttttcgcc
     5581 aaaggggaag aggaacaaac gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg
     5641 attttagaca gccgcccggt tcatgcagca tgtccacaca gtgaagctat tcgcgacgct
     5701 caacgaaaaa aacctaaagt tccggtacat gctgtactga cggcgacaaa tccgctgatc
     5761 cgttttatcg gtagtgatga tatgacgcaa aaccgggaat tatttcaggt ctggttacag
     5821 aaattagcgc agtggcatca gaccactacg ccttatcttt ttttacatac gccagacatt
     5881 gcccaggccc cggaactggt acataccctg tgggaagact tacgtaaaac gcttccagag
     5941 atcggagcag ttccggctat tccacagcaa tcttctcttt tctgaatttg ccacctatca
     6001 tagacaggtg ccatcggcca ttttaaaggg agtttgtatg gtaagcgcgc tatatgccgt
     6061 tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc gttcgcttgc gtttgcagta
     6121 ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa agcgctattc gcattcatgg
     6181 taacgcggtg gaatatattc ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg
     6241 cgcagaaacc tggatggtgc atatttgcgg catcgttttg cttgctggtc gtctgatgca
     6301 ttattacggt tttcatcacc gtctgtttcg ctggcgacgt tccggcatga gcgccacctg
     6361 gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat atgccctggg agttggtttt
     6421 ctccctgcgt tagcgcacaa tacgccactt tctttttccc ggatttttac gttatgtctc
     6481 accgcgacac gctattttct gcccctatcg ccagactggg cgactggacc tttgatgaac
     6541 gggtagctga agtcttcccg gatatgatcc agcgttccgt tcccggctat tccaatatta
     6601 tttccatgat tggtatgtta gccgagcgct tcgttcaacc tggtacgcag gtttacgatc
     6661 tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg caacattcat catgataatt
     6721 gcaaaattat tgccatcgac aactccccgg cgatgattga acgctgccgt cgtcatattg
     6781 acgcctataa agcccctacg ccagtagacg tcattgaagg tgatattcgc gatatcgcca
     6841 ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca attcctggaa ccttccgagc
     6901 gccaggcatt actggataaa atttatcaag gactgaaccc gggcggcgcg ctggtgcttt
     6961 cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact gttgttcaac atgcaccacg
     7021 actttaaacg tgccaacggt tacagcgaac tggagatcag ccagaagcgc agcatgctgg
     7081 aaaacgtgat gctgaccgat tccgtggaaa cccataaagc acgcctgcat aaagccggtt
     7141 ttgagcatag cgagctgtgg ttccagtgct ttaactttgg ttcactggtg gcattaaaag
     7201 cagaggacgc tgcatgatcg actttggtaa cttttattct ctgattgcca aaaatcatct
     7261 ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac tggcagcgcg agcagcagca
     7321 cgggctgttt aagcagtggt ccaacgcggt ggaatttctg cctgaaatta aaccgtatcg
     7381 tctggattta ttgcatagcg taaccgccga aagcgaagag ccactgagca ccgggcaaat
     7441 taaacgcatt gaaacgctta tgcggaacct gatgccatgg cgcaaagggc cgttctcact
     7501 gtatggcgtc aacatcgata ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc
     7561 ccatctttct gatttaaccg gacgcaccat tctggatgtc ggctgcggca gcggttatca
     7621 catgtggcgc atgattggcg caggcgcgca cctcgcggtg gggatcgacc ctacgcaact
     7681 gtttctgtgc cagtttgaag cggtgcgtaa actactgggt aacgatcagc gcgcacattt
     7741 gttaccgtta ggtattgaac agcttccggc actgaaagcc tttgataccg tcttttcgat
     7801 gggcgtgctt taccatcgcc gttcgccgct ggagcatctc tggcagttga aagatcaact
     7861 ggtgaatgag ggtgaactgg tgctggaaac gctggttatt gatggcgatg agaacacggt
     7921 gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct
     7981 ggcgctgaaa aactggctga agaagtgtgg ttttgtagat atccgcgttg tggatgtgtg
     8041 cgttaccact actgaagagc agcgacgcac tgaatggatg gttaccgaat ctctgtccga
     8101 tttcctcgac ccacatgatc cgagtaagac agttgaaggt tatcctgcgc ctaaacgcgc
     8161 ggtgctgatt gcgcgcaagc cgtaaaggtc tggcattact gccggatgcg gcttgaatgc
     8221 cttatccggc ctacaaaccc ttgctaattc aatctattgc agggactatg taggcctgat
     8281 aagcatagcg catcaggcag ttttacgttt gcataacctc agcgcccgtt tccgggcgct
     8341 attcacttct tacgcctgaa cagcaggctt atcaaatgcc gttaacttcg gtgcgttacc
     8401 cgtatatttt tcgatataca ccagcgcaga gttaccggca caggcatttg ccagctgcga
     8461 gctggggata tccgccgtta acacgttcgc actgccgttt ttacacaagc cgttttccag
     8521 atctggccat gcaccttcat gcacgcatac cacgcctttt ttgatcccgt cagtcaccac
     8581 cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc accagatcgc cattcgcaat
     8641 accaaatcga gcagcatctt cggtgtgaat agtgatcggt tcacgatcgg caaccgcata
     8701 ttttttacgc agttccgcat agttaagctg actatgtaaa cggtgtgccg gatgtgcggt
     8761 cagaagctgc aactgcttct cgtcggcggt acccttccac tcatcaggcg caagccaggt
     8821 tggatgtgcc gggcaatcct tatagccaaa tttttccagc gttttggagt aaatctcaat
     8881 tttgccgctt ggcgtaccca gcgcattttt caccggatcg gcgcggaaat cagcataacg
     8941 aacgtactgt tcgttcttct cgctgcggcg catttcgatc agtttatttt gctgccagaa
     9001 ggcattaaac atcggcatag tcacgcgttg cgcacgagca cctttctgag cggcatcata
     9061 gaaaaatttc agccacgcca tttcatcttt accttcggta tagatctctt ttccgccagg
     9121 tttcagtaat tcagcaagat cggcaaatac gtcaaaatcg ttacgcgctt caaattgcgg
     9181 agcaacagcc tgcttcatcg gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa
     9241 gtcattgcgc tcaaacgatg tggtgatcgg taatacgata tctgcatgtt tagcagctgc
     9301 ggtccagtag cattcagaaa cgacgatcat ctccggtttc tgccatgctt tgatcagacg
     9361 attagtatcc tggtgatggg taaagttacc gccacccgcc caccagatca ttttgatatt
     9421 cggatacgtt tgttctttac cgttatgctg atatttgcca ccaggatttt ccagcgcatc
     9481 gacgatacgg gcaacaggaa tcgccgtcat tccgccatca tcagcggcct cgctggcctg
     9541 tccggcaatc gccgcagaca tttccggcag cacgccgcca acacgcgtcg gattgccacc
     9601 gttggagtaa tgataagaga aaccaaaacc gccgcccggt gtaccaatct gccctaacat
     9661 cgccgccagc gtcaccagca tccagtgttt ctgttcacca tattgctggc gctgaattcc
     9721 ccagccagcc atcagcattg tgcgattcgc cgccatcagt tcagcgagtt tgactatctg
     9781 cgcttccggt acgccagtaa tttccgccgc ccaggccgcg ctcttcggag tattatcgct
     9841 cttacctgtc agatactctt caaactgcgg atacccggtg gtgtattttt caaggaacac
     9901 tttatcgtgt ttaccttgtg tcatcagggt atgcgcaatc cctaacatca gtgccacgtc
     9961 ggtgcccata ttcggcgcga tccaggtggc attatcacca aagaactcga tggtttcgga
    10021 gcggatagga tcaatggcaa tcactggttt gccagatttt ttcagctgat ggaagtattc
    10081 cagcccttgc tcatcggtac tgctccaggc aatttttaag gtattcagcg ggttcatccc
    10141 ccacagcacc acagcctggc tgttttccag aatcagcggc caggaggtct gctgttcata
    10201 cacctctaca gaaccgacca catgcggcat gatcacctgt gctgccccgg ttgaataatc
    10261 gccgctatgc ccggaataac cgcccgccag gttcatataa cgttgcagta aggtttgcgc
    10321 tttatgcaac acgccagaag agcgccagcc gtatgatccg gcaaaaatag cagatggtcc
    10381 gttagctttg cgaatgcgat cgtgttgctc atgaatcagt tttaatgctt gctcccagct
    10441 cacctgtaca taagtatctt cgccacgacc tttcgccggt tgcagtggat tatcgagata
    10501 gctttttctt accatcggat gctgaatacg tgccgtggtg tgtacctgat ccgccgccgt
    10561 agactgtaag gagttcggta tggttttcgc cagcgcgcct gttgaagaaa caatcttgcc
    10621 gtccttcact tctacgttca tcgctcccca acgtcccgcg gtgaggattt taccgccctt
    10681 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc accagcgctc cagcggcaat
    10741 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
    10801 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
    10861 cagttcgttt tcgctcatgc tggttcgcgc acccattcct ttggcaatgg acggccatgc
    10921 attgacggtg taatggtcag cggcaatagg cgcatggcaa ccagcgcaat aggtatcgtc
    10981 aagtttttca gcgtattgcc atagcggttt acggtccgct aatgcgggat cggtaagcgc
    11041 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
    11101 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
    11161 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
    11221 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
    11281 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
    11341 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
    11401 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
    11461 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
    11521 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
    11581 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
    11641 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
    11701 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
    11761 aggtttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
    11821 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
    11881 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accgaaatca
    11941 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
    12001 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
//
LOCUS       DAZFKM010000146        11852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFKM010000146 DAZFKM010000000
VERSION     DAZFKM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..409
                     /locus_tag="ACHKAY_004236"
     CDS             <1..409
                     /locus_tag="ACHKAY_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5702811.1"
                     /translation="LFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLA
                     ILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEI
                     NNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            733..2121
                     /gene="narU"
                     /locus_tag="ACHKAY_004237"
     CDS             733..2121
                     /gene="narU"
                     /locus_tag="ACHKAY_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO5702812.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2203..5943
                     /gene="narZ"
                     /locus_tag="ACHKAY_004238"
     CDS             2203..5943
                     /gene="narZ"
                     /locus_tag="ACHKAY_004238"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO5702813.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5940..7484
                     /gene="narH"
                     /locus_tag="ACHKAY_004239"
     CDS             5940..7484
                     /gene="narH"
                     /locus_tag="ACHKAY_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO5702814.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7484..8179
                     /gene="narW"
                     /locus_tag="ACHKAY_004240"
     CDS             7484..8179
                     /gene="narW"
                     /locus_tag="ACHKAY_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO5702815.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8176..8856
                     /gene="narI"
                     /locus_tag="ACHKAY_004241"
     CDS             8176..8856
                     /gene="narI"
                     /locus_tag="ACHKAY_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO5702816.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8935..9828
                     /locus_tag="ACHKAY_004242"
     CDS             8935..9828
                     /locus_tag="ACHKAY_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO5702817.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9924..10769)
                     /gene="nhoA"
                     /locus_tag="ACHKAY_004243"
     CDS             complement(9924..10769)
                     /gene="nhoA"
                     /locus_tag="ACHKAY_004243"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO5702818.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10942..11511
                     /locus_tag="ACHKAY_004244"
     CDS             10942..11511
                     /locus_tag="ACHKAY_004244"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO5702819.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11515..11742)
                     /gene="pptA"
                     /locus_tag="ACHKAY_004245"
                     /pseudo
     CDS             complement(11515..11742)
                     /gene="pptA"
                     /locus_tag="ACHKAY_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2844 a         3045 c         3086 g         2877 t
ORIGIN      
        1 tttattcccc ataataaaac agagcaaaaa cttaaatttt ttaaatgttt ctgggaagaa
       61 caattgccct actatgcagc tcatgttatt taatttgttt tccccagcac ttaagcttaa
      121 tactggcctg gcaattcttt cgcctggtgc atttgaagtt cactctgacg gaatagatgt
      181 ggataacgaa ttgtttcact atcctattaa aaaagcatat accccatata atatacacac
      241 ttacaagaca gaggaagttg taaaccagag gaatataaaa gttaaaaaca tgaccttaga
      301 tgaaataaac aatacttact gtaataacga ttattacaat caggcaataa gagaggaacc
      361 gatagacctt ctggacagat cgttttcctc cagttcatgg cctttttagt gaataaatca
      421 tcactaattc tataagtgtc tggtcttgtt gataatatag ttaatatgta accctttaga
      481 ttcacgatct gaagggttga aacatgacat acgttcaaag attaaccccc gtttgaaggt
      541 tagcttgtta gttacattta gtaacacata tctggctact taatccccat tctgcccccg
      601 cggttacgct ttcttatcta ttctttcaat cagcacttca cccaattcat ccttaattct
      661 aataactggc tattgcgcct tacctcctgt tttgtctttt ttcattacca atgtgtgcat
      721 gtgaggaaca atatggcact gcaaaatgag aaaaatagtc gttatctttt acgcgactgg
      781 aaaccagaaa atccggcctt ctgggaaaat aaaggaaaac atattgctcg aagaaacctc
      841 tggatatcag tcagttgtct acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt
      901 accgttaatc tcaataaaat cggttttaat tttactaccg atcaactctt tttattaacc
      961 gcattaccct ccgtttctgg cgcattattg cgtgttccct actcctttat ggtgcctata
     1021 ttcggtggac gccgatggac ggtttttagt actgcaatcc tgattattcc ttgcgtctgg
     1081 ctcggaattg ccgtgcaaaa tccgaatact ccttttggga tatttatcgt tatcgctttg
     1141 ctatgcggtt ttgcaggtgc aaactttgct tcgagcatgg gcaatatcag tttcttcttt
     1201 ccaaaagcca aacaagggag cgctcttggg attaatggcg gattaggaaa cttaggtgta
     1261 agtgtaatgc agctggttgc accgctggtc atttttgtac ctgtatttgc ctttctcggc
     1321 gtcaatggcg taccgcaggc cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt
     1381 tgggtaccgc tactggcgat tgccacgatc gccgcatggt cagggatgaa tgatatcgcc
     1441 agttcacgcg cctcaattgc cgaccagctc cctgtcttac aacgcctgca tctctggctg
     1501 ctgagcctgc tttaccttgc caccttcggt tcgtttatcg gtttttctgc gggttttgcc
     1561 atgctggcaa aaacccagtt cccggatgtg aatattctgc gcctggcgtt ctttggccca
     1621 tttatcggtg ccatcgcgcg gtcggttggt ggtgctattt ccgataagtt cggcggcgtg
     1681 cgggtgacgt tgatcaactt tatttttatg gcgattttca gtgccctgct gttccttacc
     1741 ttaccgggca caggctccgg taatttcatc gccttttacg ccgtatttat ggggctgttt
     1801 ctgactgcgg gtctgggaag tggttctact ttccagatga tcgccgtcat ctttcgccag
     1861 ataaccattt atcgggtaaa gatgaaaggc ggtagtgatg agcaagctca gaaagaagcc
     1921 gtcaccgaaa cggcggcggc tctgggcttt atctcagcca ttggcgcagt gggcggcttt
     1981 tttattccgc aggcgtttgg catgtcgctc aatatgaccg gctctccggt cggcgcgatg
     2041 aaagtgtttt taatcttcta catcgtttgt gtgctgctga cctggctggt ttatggtcgg
     2101 cggaagttca gccaaaaata agagatcgat gcctgatgta caaccgtgtc gcatccgaca
     2161 aggcatcaga tcccaattca ttcctggagc aggagtcatg tcatgagtaa acttttggat
     2221 cgctttcgct acttcaaaca aaagggcgaa acctttgccg atggtcacgg acaggtgatg
     2281 catagcaacc gcgactggga ggacagctat cgccagcgtt ggcagttcga caaaatcgtg
     2341 cgttccaccc acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat
     2401 ggtctggtga cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgccc
     2461 aatcatgaac ctcgcggctg cccgcgtggc gcaagttact cctggtatct ttacagcgct
     2521 aaccgcctga aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc
     2581 aagcaacaca gcgatccggt actggcgtgg gcatcgatta tgaacgatcc gcaaaagtgc
     2641 ctgagctaca aacaagtgcg tgggcgcggc gggtttatcc gctccaactg gcaggaacta
     2701 aaccagctga ttgccgccgc taacgtctgg accatcaaaa cctacggccc ggatcgcgtt
     2761 gccggtttct cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacgcgttat
     2821 ctgtcgctgc ttggcggcac ctgtttaagt ttctacgact ggtattgcga cctgccgccc
     2881 gcctcgccga tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac
     2941 tccagctaca tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccac
     3001 ttctttaccg aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct
     3061 gaagtggcca aattgtgcga ccagtggctg gcaccgaaac aaggcactga tagcgccctg
     3121 gcgatggcaa tgggccatgt gattttaaaa gagtttcatc tcgataatcc cagcgactac
     3181 tttatcaact actgccgccg ctacagcgac atgccgatgc tggtaatgct ggagcctcgc
     3241 gacgatggta gctacgttcc cgggcgcatg atccgcgcat ctgacctggt ggatggactg
     3301 ggcgaaagca acaatccgca gtggaaaacc gtagcagtta ataccgcagg tgaattggta
     3361 gtgccgaacg gttcgattgg tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc
     3421 attgccgccg gtacggaaac cgaattgtcg ttaaccctgc tcggtcaaca tgacgctgtt
     3481 gcaggcgtgg ccttccccta ctttggcggc attgaaaatc cgcattttcg cagcgtaaaa
     3541 cacaatccgg tgctggtgcg ccaattgccc gttaaaaacc tgacgttagt cgatggcaac
     3601 acctgtccgg tggtcagcgt ttatgatttg gtactggcga attacggcct cgatcgcggg
     3661 ctggaagatg aaaacagtgc gaaagattac gctgaaatca aaccgtacac cccagcctgg
     3721 ggtgagcaaa ttaccggcgt gccgcgccag tatattgaaa ccatcgcccg tgaatttgcc
     3781 gataccgccc ataaaacgca tgggcgctcg atgattatcc tcggcgcagg tgttaaccac
     3841 tggtatcaca tggacatgaa ctaccgcggg atgatcaata tgctgatctt ctgcggttgt
     3901 gtcgggcaaa gcggtggcgg ctgggcacac tatgtcggcc aggaaaaact gcgcccacaa
     3961 accggctggt tgccgctggc ctttgcgctc gactggaacc gaccaccacg ccagatgaac
     4021 agtacctcgt ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgctcag
     4081 gagttacttt caccgctcgc cgatgccagt aagtacagcg gtcatctgat tgatttcaac
     4141 gttcgcgccg aacgtatggg ctggctacct tctgcgccgc agctggggcg taacccgctc
     4201 gggattaaag ctgaagccga caaggccgga ttatccccca cagaatttac cgcccaggcg
     4261 ctgaaatcgg gcgatttacg tatggcctgc gaacaaccag atagcggcag caatcatccg
     4321 cgtaatttgt ttgtctggcg ctctaacctg cttggctcct ccggcaaagg ccacgagtat
     4381 atgcagaagt atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc
     4441 gacgggatca aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac
     4501 ctgctggtga cgctcgactt ccgcatgtcc agtacctgcc tgttctccga tatcgttctg
     4561 cccaccgcca cctggtacga aaaagacgat atgaacacct cggatatgca tccgtttatt
     4621 catccgcttt ctgcggcggt cgatccggcg tgggaatcac gcagcgactg ggaaatctac
     4681 aaaggtatcg ccaaagcatt ttcgcaagtg tgcgtgggtc atcttggcaa agaaaccgac
     4741 gtggtattac aacccctact gcatgactct ccggcagagc tctcacagcc gtgtgaagtg
     4801 ctcgactggc gcaaaggcga atgcgatctg attccaggca aaaccgcgcc gaatattgtg
     4861 gcggtggagc gcgactaccc tgctacgtat gaacgcttta cctcgctcgg gccattgatg
     4921 gacaaacttg gcaatggcgg taaagggatt tcgtggaata cgcaggatga aatcgatttc
     4981 ctcggcaaac tcaattacac caagcgtgat ggcccggcac aggggcgtcc gctgattgac
     5041 accgccattg acgcttcaga agtgattctg gcactggcac cggaaaccaa cggtcatgtt
     5101 gcagtcaaag cgtggcaggc gctgggcgag atcaccgggc gcgaacatac ccatctggcg
     5161 ctgcacaaag aggacgagaa gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt
     5221 atctccagcc ccacctggtc cggtctggaa agcgatcacg tatcctataa cgcgggatac
     5281 accaacgttc atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag
     5341 gatcatccgt ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc gcctatcgac
     5401 acccgtagcg tcagtgagat gcgccagata ccgccaaacg gcttcccgga aaaagcactt
     5461 aacttcctga cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta
     5521 atgctgacgc tctctcgcgg tggaccgatt gtctggatca gcgaaacaga tgcccgagaa
     5581 ctgaccattg tcgataacga ctgggtggaa gtattcaacg ccaacggcgc gctgactgcc
     5641 cgcgcggtgg tcagccaacg tgtaccgccg ggcatgacca tgatgtatca cgcccaggaa
     5701 cgcattatga atattcctgg ttcggaagta actggcatgc gcggcggcat tcataactcg
     5761 gtcacccgcg tttgcccgaa accaacgcat atgattggcg gttacgcgca gctggcctgg
     5821 ggctttaact actacggcac cgtcggatcg aaccgcgatg agttcatcat gatccgcaag
     5881 atgaagaacg ttaactggct ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa
     5941 tgaaaatccg ttcacaagtc ggcatggtgc ttaacctcga caaatgtatc ggctgccata
     6001 cctgttcggt gacctgtaaa aacgtctgga ccgggcgcga aggcatggag tacgcatggt
     6061 ttaacaacgt cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag
     6121 agtggcaagg cggctgggtc cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca
     6181 agatgggcgt aataaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact
     6241 acgaaccttt caccttcgac tacgaacatt tgcatagcgc accggaaggc aaacatatcc
     6301 ctactgctcg cccgcgttca ctgattgacg gcaaacggat ggacaaagtg atctggggcc
     6361 caaactggga agaactgctg ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg
     6421 aggccatgca aaaggagatg tacgggcagt ttgaaaacac cttcatgatg tacctgccgc
     6481 gcctgtgcga acactgcctc aatcccagtt gcgtggcgac ctgcccaagc ggcgctatct
     6541 acaaacgcga agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt
     6601 tgtgcattag cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag
     6661 aaaaatgtat cttctgttat ccgcgaattg agtcaggcca acccaccgtg tgctcagaaa
     6721 cctgcgtggg tcgcatccgg tatctgggcg tgctgcttta cgacgccgac cgcattgagg
     6781 aagcggcgag caccgagcgc gaagttgacc tctatgaacg tcagtgcgaa gtattcctcg
     6841 atccacacga tccctcagtg atcgaggaag ccctgaaaca aggtattcca caaaacgtga
     6901 ttgaagctgc ccagcgttcg cctgtctaca aaatggcgat ggactggaaa ctggcgctac
     6961 cgctgcaccc tgaatatcgc accctgccaa tggtctggta cgttcctccg ctgtcgccga
     7021 ttcagtccta cgcagatgcg ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg
     7081 aaagcctgcg tattccggtg caatatctcg ccaatatgtt gagtgccggc gataccggtc
     7141 cggtactgcg ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg
     7201 tggaaggcgt tactgatact cgtgccatcg acgaagtagg cctgagcgtc gcccaggtcg
     7261 aagagatgta tcgttacctc gccattgcca actatgaaga tcgttttgtc atcccgacga
     7321 gccatcggga aatggcgggc gatgccttcg cagaacgcaa cggctgcggt tttacctttg
     7381 gcgacggttg ccacggttcg gacagtaaat tcaacctgtt caacagtagc cgtatcgatg
     7441 ccatcaacat caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt
     7501 gatcggcctg ttgatggagt atccggacga gctgttgtgg gaatgcaagg aggacgcgct
     7561 ggcgttgatc cgcagcgacg cgccgatgct tacggatttc acccacaacc tgcttaacgc
     7621 gccgctgctg gataaacagg ccgaatggtg cgaagtgttt gaccgtggac gtaccacatc
     7681 gctgctgctg ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga
     7741 cctgctggcg gagtatgaaa aggtcggcct gcagctggat tgtcgggaac tgcccgatta
     7801 tctaccgctg tatctggagt atttaagcgt actgcctgac gatcaggcga aagaaggatt
     7861 gctcaatgtt gcgccgatcc tcgccctgct tggcggtcgc ttaaaacaac gcgaggcacc
     7921 gtggtacgcg ttgtttgatg ctctgctgca actggcggga agctcccttt caagtgacag
     7981 tgtcacaaaa caagtaaaca gcgaagagcg cgatgacacc cgccaggcgc ttgatgcagt
     8041 gtgggaagag gaacaggtga agtttattga agataacgcc acggcgtgtg acagctcgcc
     8101 gcttaatcaa tatcagcgac gctttagcca ggatgtcgcg ccgcaatatg tcgacatcag
     8161 tgcgggaggt gggaaatgat tcagtatctg aacgtctttt tttacgatat ctacccgtac
     8221 atttgtgcga cggtgttttt cctcggcagc tggctgcgct acgactacgg gcagtacacc
     8281 tggcgcgcct cctcaagtca aatgctcgat aaacgcgaga tggtgatatg gtcgaatctg
     8341 ttccatatcg gcattttggg gattttcttc gggcacttgt tcggcatgtt aacgccgcac
     8401 tggatgtacg cgtggttttt gccagtggca gcgaaacaac taatggcgat ggtgctcggc
     8461 ggtatttgcg gtgtgttgac attgattggc ggcgcaggac tgctgtggcg caggctgaca
     8521 aatcagcgcg tgcgtgcaac ttccactacg ccggatatta tcatcatgag cattttgctg
     8581 atccagtgtc tgctggggct aagtaccata ccgttttcag cacagtatcc tgatggtagc
     8641 gaaatgatga agctggtggg ttgggcgcaa agcattgtca ctttccgtgg tggatcgtca
     8701 gaaatgctta acggcgtagc gttcgttttc cgcctgcatc tggtattggg aatgaccatc
     8761 ttcctgctct tcccgttcac cagattggtg catgtgtgga gcgcgccgtt tgagtacttt
     8821 actcgtcggt atcagattgt gcgttcgcgg cgataatttc accctggccg gatagccctt
     8881 cgctctccgg ccacttgcag gctatgatag ttctccagtc acaggagaac gttaatgaaa
     8941 ccgcaggtgt accacgtcga tgcttttacc tctcaaccat ttcgcggcaa ttctgccggt
     9001 gttgttttcc ccgccgataa tcttagcgaa gcgcaaatgc agcttatcgc ccgcgagtta
     9061 ggccattcgg aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg catccgctac
     9121 tttacgccaa cggttgaagt gccgatttgc ggtcacgcga cagtagctgc gcactatgta
     9181 cgtgcgaagg tgttaggttt aggaaattgc acgatctggc aaacatcgct ggcaggaaaa
     9241 catcgcgtga ctatcgaaaa gcacaacgat gattatcgta tttcgctgga acaaggtacg
     9301 ccgggctttg agccaccgct ggaaggtgaa acacgtgagg cgattatcaa cgcgcttcat
     9361 ctcactgaag acgatatttt gccgggcttg ccaattcagg tcgcaacaac aggacactca
     9421 aaagtgatga tcccgttgaa accggaagtg gatatcgacg ccctttcgcc tgatcttgct
     9481 gcgctgagcg ctatcagcaa acagattggc tgcaatggtt tcttcccgtt ccagatccgc
     9541 ccaggcaaaa acgaaaccga tggtcgtatg ttctcgcctg cgattggtat tgtggaagat
     9601 ccggtgactg gcaatgccaa tggcccgatg ggtgcatggt tggtacatca caacgtattg
     9661 ccccacgatg gcaacgtgtt atgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgac
     9721 ggcatgattg aagtgacggt gacaattcgt gataaccaac cggaaaaagt caccatttca
     9781 ggcacagcgg tgattttgtt tcatgccgaa tgggcaattg aactctgaaa ccgttgcctg
     9841 atgcgcttcg cctgtcaggt ctacaaccga gctaatgtat gccggataag gcgtttacgc
     9901 cgcatccggc aacctgacat aaattatttt cccgcctccg ggtgcgtatc aaacgccgcc
     9961 atcaccagcg ctaactcatc cacggtaaag ccatgtttcg catcatccac gcccagacca
    10021 aactgttctt gcatcacagc atataatgac gccacatccg gtagatttcg ctgctccacc
    10081 gcgtgcccat tttcataatg ggtaaaatga aaattggtca gcgttagctt gccaccgtcc
    10141 ggcaaatggc ggcacatcag caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac
    10201 cagaaattgc ccatcacata atcgctttgt tgctgctcgc agagatcaaa acggtacatc
    10261 gactgccagt gctgatgatg attaaactgc aacacccaat catcaccctc ctgcaacaac
    10321 cgataatctc cgtgtggcgt ggtctgcacg agatcggaaa ctaaacgaat cggcgcggtt
    10381 agcgtctgcc caccgaaacc gacatcagca atccattttt cctcttccag ttccaccaac
    10441 agcaaacgat gggtgcgcgg cggtaatgct ggcggatttg ataacactac gcgccctaac
    10501 aagctgcgaa cgttaaactc cagctcgcgt aacacccgct caaacacgcc attctgctca
    10561 aaacagtaac cgccacgacg ggctatcacc agtttctctt ccagcgattg atcatcaagc
    10621 tgtatttccc tcggcagcaa aacgtcgagg ttttcaaacg gaatagtgca attgtgtttc
    10681 aggtgcaatg cacgaagcgt atcaatattg accgcagcag ctcccgacca gttaatacgg
    10741 gcaaaatagt gattcagaat gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt
    10801 ataacctgga atagacgccg caccggctat tttgtgcgat cgcggacatg catcacgttt
    10861 gcaaatcgcc aatcccgtta atttcgttac ttattgcgtt acggactacg cttaaaaaaa
    10921 ccttcttcgc gaggcgatac catgagccga ttcatcccca tagaattaca ccatgccagc
    10981 cgtctgttaa atcatggccc aactgtcatg atcaccagtt tcgatgagca atcccagcgg
    11041 cgtaacatta tggcagcagc ctggtcaatg ccggtggagt ttgaaccgcc acgcgtggcg
    11101 attgtggtag ataaatcgac atggaccagg gagttgattg aacgtaacgg taaatttggc
    11161 atcgttatcc cgggcgttgc agcaactaac tggacgtggg cagtgggaag cgtgtcgggt
    11221 cgtgaagaag ataaatttaa ttgctatggc attccggtgg tgaaaggtcc ggtgcttggt
    11281 ttgccacttg ttgaagagaa atgcctggcg tggatggagt gccgcctgct acctgcgact
    11341 tctgcgcaac aaaaatacga cacgctgttt ggtgaggtgg tatcagcagc ggcagacgca
    11401 cgggtatttg tcgaaggccg ctggcagttt gatgatgata agctcaatac gttgcatcat
    11461 ttaggtgctg gcacgtttgt taccagcggc aagcgagtta cggcgggtta agcattacat
    11521 gctgtaaccg ggtttcttta tcaaagcctc catttggggc gcgatttcgg catcccagat
    11581 agcttgccaa gattctggtt gaatctgctg tagagcaatg cttatcgagc tatctttact
    11641 gttcagatgg cgaataataa cgtcggtaat atctgcagca agtgctgctt tttattgttc
    11701 gtccagttca cgcggaaaac atttaatgtc gatgtgcggc atgattgtgc tccttttgaa
    11761 agatgtcagt aaaattaaca cacattgtga atgtcgtgtg tccggtaatg cgtttttacg
    11821 acaataatgc cagggcaaga ttacgaaagc ct
//
LOCUS       DAZFKM010000147        11589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFKM010000147 DAZFKM010000000
VERSION     DAZFKM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            197..1339
                     /gene="yghO"
                     /locus_tag="ACHKAY_004246"
     CDS             197..1339
                     /gene="yghO"
                     /locus_tag="ACHKAY_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO5702820.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1346..2110)
                     /gene="glcC"
                     /locus_tag="ACHKAY_004247"
     CDS             complement(1346..2110)
                     /gene="glcC"
                     /locus_tag="ACHKAY_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO5702821.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2361..3860
                     /gene="glcD"
                     /locus_tag="ACHKAY_004248"
     CDS             2361..3860
                     /gene="glcD"
                     /locus_tag="ACHKAY_004248"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO5702822.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3860..4912
                     /gene="glcE"
                     /locus_tag="ACHKAY_004249"
     CDS             3860..4912
                     /gene="glcE"
                     /locus_tag="ACHKAY_004249"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO5702823.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4923..6146
                     /gene="glcF"
                     /locus_tag="ACHKAY_004250"
     CDS             4923..6146
                     /gene="glcF"
                     /locus_tag="ACHKAY_004250"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO5702824.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6151..6555
                     /gene="glcG"
                     /locus_tag="ACHKAY_004251"
     CDS             6151..6555
                     /gene="glcG"
                     /locus_tag="ACHKAY_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO5702825.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6577..8748
                     /gene="glcB"
                     /locus_tag="ACHKAY_004252"
     CDS             6577..8748
                     /gene="glcB"
                     /locus_tag="ACHKAY_004252"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO5702826.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8976..9221
                     /gene="traT"
                     /locus_tag="ACHKAY_004253"
                     /pseudo
     CDS             8976..9221
                     /gene="traT"
                     /locus_tag="ACHKAY_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9254..10996)
                     /gene="cptA"
                     /locus_tag="ACHKAY_004254"
     CDS             complement(9254..10996)
                     /gene="cptA"
                     /locus_tag="ACHKAY_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5702827.1"
                     /translation="MQKTLTKHEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTN
                     GLRDSLLFSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLF
                     VMFESNTSEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFII
                     YGLILHPVLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEE
                     NNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMF
                     VFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGT
                     HIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKAT
                     APDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFS
                     EYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYD
                     TLPYAELRVNQASN"
     gene            11453..>11589
                     /locus_tag="ACHKAY_004255"
     CDS             11453..>11589
                     /locus_tag="ACHKAY_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5702828.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ET"
BASE COUNT         2907 a         2791 c         3119 g         2772 t
ORIGIN      
        1 ttaagaagaa gccttgtccg gtcgccttaa atcgacaaaa ggcattgagg cgcttctgtg
       61 tagtctctaa ccttatcata atcattaata aatactattt attctttgtt tgtattcatt
      121 gggttgatta ttaaggtgtc gttcgttgct gttgcaacct gtttttcacc gttaattatt
      181 tgttatccac agagatatgg aatgcgatct gctgatgatt aaaattgaaa aagtaattaa
      241 taaaaatgac cttaaggcat ttatcgcttt tccgtcatca ctttatcccg acgatccaaa
      301 ctggataccc cctttattca tagaacgcaa cgagcatttg tctgcgaaaa atccggggac
      361 ggaccatatc atctggcagg cgtgggtggc aaaaaaagcg gggcagatag ttgggcgtat
      421 taccgcgcaa atagatacct tgcaccgtga gcgttacggt gaagataccg gtcatttcgg
      481 catgattgac gccattgatg atccgcaggt ttttgccgcg ttgtttggcg cagcggaagc
      541 gtggttgaag tcacaaggtg caagtaagat cagcggtcct ttcagcctga atatcaatca
      601 ggaaagcgga ttactgattg aaggttttga cacaccaccc tgtgcgatga tgccacacgg
      661 caaaccgtgg tatgccgcgc atattgaaca actgggttat cacaaaggca ttgatttact
      721 ggcgtggtgg atgcagcgaa ccgatctcac tttctctccg gcgctaaaaa aactgatgga
      781 tcaggtgcgc aaaaaggtga ccattcgctg catcaatcgt cagcggtttg ccgaagaaat
      841 gcagatcctg cgtgagattt tcaactctgg ctggcagcac aactggggat ttgtgccgtt
      901 taccgaacat gaattcgcga ccatgggcga tcaacttaaa tatctggtgc cggacgatat
      961 gatctatatc gctgagattg attctgcacc ctgcgcgttt attgtcggct tgccgaacat
     1021 caacgaggcg attgccgatc tgaacggatc gctctttccc ttcggctggg caaaattgct
     1081 gtggcgtttg aaagtcagcg gtgtgcgaac tgcgcgagta ccgctgatgg gcgtgcgcga
     1141 cgagtatcag ttcagccgca tcggcccggt gattgcgctg ttattgattg aagccttacg
     1201 cgatccgttt gcccgccgga agatcgatgc gctggagatg tcatggatcc tcgaaaccaa
     1261 tacaggcatg aataacatgc tggaacgcat tggggcggag ccgtacaagc gttaccgatt
     1321 gtatgaaaag cagatttaat accagctaac tcaggttcat ctccagcggc accgccgagc
     1381 gaatcaaatg gtggccttcc agctcgatat cgtggagatt ctttttcacg gtccgcacat
     1441 gatcgcgtgc tgcgcgctgg gcgacgtgcg gcagccgctg caacaccgcg ttgtagatcc
     1501 tcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg atggtagaga ttatttacgc
     1561 tggcaaacac tgaattaaac atcagatcgg tcaatgattg cagcgtaaac accagcacct
     1621 gattgtgaga agcctgacaa atggcgagat ggaaagcgtg atccaactgc gcatgttcga
     1681 tcagcgaaat ctctttgttg ttctcactgg cggcgagcat tttttcataa cagcgggtta
     1741 tcacaacaaa atcagcctgc gttcccagcg ttgccgccag ccttgccgat tcgccctcca
     1801 gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg ctgcgtactg aacagatgga
     1861 tcagcggact ggtgtcctgc acccgattaa gccgtgcgac acgagaatcg cgaccctgcg
     1921 ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc ttcacgcagt gcggagcgtg
     1981 agaagccgag cttttcacac agtcgacgtt ccgagggaag tggctgaccg accttcagta
     2041 cgccgtcgat aattaaccgt tcgatactct ctgcaaccac ttcgcaaata gggcgacgtt
     2101 catctttcat tcccggacct cgtgcacagg taggaccaat ttttctgttc ttaactcgca
     2161 aaacacgcac atcacgtaag tgattatgtt acatcaattt aacattgagt taaccaaggc
     2221 aaggtcacag agctggaaaa aaaatggtct gaccggtagc taaagagata gacgaaaacg
     2281 aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa aaatcagctg ccacacaaca
     2341 caacaaagcg aagcctactc atgagcatct tgtacgaaga gcgtcttgat ggcgctttac
     2401 ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga gcatgtccct ggacttgaga
     2461 tcctgcatac cgatgaggag atcattcctt acgagtgtga cgggttgagc gcgtatcgca
     2521 cgcgtccatt actggttgtt ctgcctaagc aaatggaaca ggtgacagcg attcttgctg
     2581 tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc aggcaccggg ctttctggtg
     2641 gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc gcgctttaaa gagatcctcg
     2701 acattaaccc cgttggtcgc cgcgcgcgcg tgcagccagg cgtgcgtaac ctggcgatct
     2761 cccaggccgt tgcaccgcat aatctctact acgcaccgga cccttcctca caaatcgcct
     2821 gttccattgg cggcaacgtg gcagaaaatg ccggcggcgt ccactgcctg aaatatggtc
     2881 tgaccgtaca taacctgctg aaaattgaag tgcaaacgct ggacggcgag gcgctgacgc
     2941 tgggatcgga cgcgctggat tcacctggtt ttgacctgct ggcgctgttc accggatcgg
     3001 aaggtatgct cggcgtgacc accgaagtga cggtaaaact gctgccgaag ccgcccgtgg
     3061 cgcgggttct gttagccagc tttgactcgg tagaaaaagc cggacttgca gttggtgaca
     3121 tcatcgccaa tggcattatc cccggcgggc tggagatgat ggataacctg tcgatccgcg
     3181 cggcggaaga ttttattcat gccggttatc ccgtcgacgc cgaagcgatt ttgttatgcg
     3241 agctggacgg cgtggagtct gacgtacagg aagactgcga gcgggttaac gacatcttgt
     3301 tgaaagcggg cgcgactgac gtccgtctgg cacaggacga agcagagcgc gtacgtttct
     3361 gggccggtcg caaaaatgcg ttcccggcgg taggacgtat ctccccggat tactactgca
     3421 tggatggcac catcccgcgt cgcgccctgc ctggcgtact ggaaggcatt gcccgtttat
     3481 cgcagcaata tgatttacgt gttgccaacg tctttcatgc cggagatggc aacatgcacc
     3541 cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc ccgcgcggaa gagctgggtg
     3601 ggaagatcct cgaactctgc gttgaagttg gcggcagcat cagtggggaa catggcatcg
     3661 ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag cgatgaaatc acgaccttcc
     3721 atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa ccctgggaaa aacattccca
     3781 cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca tcacggtcat ttacctttcc
     3841 ctgaactgga gcgtttctga tgctacgcga gtgtgattac agccaggcgc tgctggagca
     3901 ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt cagggcagca atagcaaagc
     3961 ctttttaggt cgccctgtca ccgggcaaac gctggatgtg cgttgtcatc gcggcattgt
     4021 taattacgac ccgaccgagc tggtgataac cgcgcgtgcc ggaacgccgc tggtggcgat
     4081 tgaagcggcg ctggagagcg ccgggcaaat gctcccctgc gagccgccgc attatggtga
     4141 agaagccacc tggggcggga tggtcgcctg cgggctggcg gggccgcgtc gcccgtggag
     4201 cggttcggtc cgcgattttg tcctcggcac gcgcatcatt accggcgctg gaaaacatct
     4261 gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac gatctctcac ggttaatggc
     4321 cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg atgaaagtgt taccgcgacc
     4381 gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa gaagccatta acgaaatcgc
     4441 ccagtggcaa ctccagccgc tacccattag tggcttatgt tacttcgaca atgcgttgtg
     4501 gatccgcctt gagggcggcg aaggatcggt aaaagcggcg cgtgaactgc tggggggcga
     4561 agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa caactgccgt tcttctcgtt
     4621 accaggtacc ttatggcgca tttcattacc cagcgatgcg ccgatgatgg atttacccgg
     4681 cgagcaactg atcgactggg gcggagcgtt acgctggctg aaatcgacag ccgaggacaa
     4741 tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg acccgcttta gtgccggaga
     4801 tggtggcttt gccccgctat cggctccttt attccgctat caccagcagc ttaaacagca
     4861 gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac gcggaacttt gaggagcagg
     4921 ctatgcaaac ccaattaact gaagagatgc ggcagaacgc gcgcgcgctg gaagccgaca
     4981 gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc aacctgccca acctatcagc
     5041 ttctgggcga tgaactggac gggccgcgcg ggcgcatcta tctgattaaa caggtgctgg
     5101 aaggcaacga agtcacgctt aaaacacagg agcatctcga tcgctgcctc acttgccgca
     5161 attgtgaaac cacctgtcct tctggtgtgc gctatcacaa tttgctggat atcgggcgtg
     5221 atattgtcga gcagaaagtg aaacgcccac tgccggagcg aatgctgcgc gaaggattgc
     5281 gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac gcaggtaggg ctggtgctgc
     5341 gaccgttttt accggaacag gtcagagcaa aactgcctgc tgaaacggtg aaagctaaac
     5401 cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct ggaaggctgc gcccagccta
     5461 cgctttcgcc caacaccaac gcggcaactg cgcgagtgct ggatcgtctg gggatcagcg
     5521 tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga ctatcatctt aatgcgcagg
     5581 agaaagggct ggcacgggcg cgcaataata ttgatgcctg gtggcccgcg attgaagcag
     5641 gtgccgaggc aattttgcaa accgccagcg gttgcggcgc gtttgtcaaa gagtacgggc
     5701 agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg tcaagtcagt gaactggcgg
     5761 tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa actggcagtt cgcggcgata
     5821 aaaaactggc cttccactgc ccgtgtaccc tacaacatgc gcaaaagctg aacggcgaag
     5881 tggaaaaagt gttgcttcgt cttggattta ccttaacgga cgttcccgac agccatctgt
     5941 gctgcggttc agcgggaaca tatgcgttaa cgcatcccga tctggcacgc cagctgcggg
     6001 ataacaaaat gaatgcgctg gaaagcggca aaccggaaat gatcgtcacc gccaacattg
     6061 gttgccagac gcatctggcg agcgccggtc gtacctcagt gcgtcactgg attgaaattg
     6121 tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta aagtcattct tagccagcaa
     6181 atggcgagtg caattattgc cgcaggtcag gaagaggcgc agaaaaataa ctggtcggtt
     6241 tccattgctg ttgccgatga cggcggtcat ctgctggcgt taagtcgcat ggacgattgc
     6301 gcgccgattg cggcttacat ctcccaggag aaagcgcgta ccgccgcgct ggggcgtcgt
     6361 gaaactaagg gctatgaaga gatggtgaac aacggacgta ccgcgttcgt gactgcgccg
     6421 ttactgacgt cgctggaagg cggcgtaccg gttgttgtgg atgggcaaat tattggtgcc
     6481 gtgggcgttt ctggtttaac cggagcacag gatgcgcagg tcgcgaaagc ggcagcagcg
     6541 gtgttggcga aataagcgaa aacgaggaga gaaacaatga gtcaaaccat aacccagggc
     6601 cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg aagaagtttt accgggaaca
     6661 gggctggacg ctgcggcgtt ctggcgcaat tttgatgaga tcgttcacga tctggcacca
     6721 gaaaatcgtc agttgctggc agaacgcgat cgcattcagg cagcgcttga tgagtggcat
     6781 cgcagcaatc cggggccggt aaaagataaa gcggcctata aatctttcct gcgtgaactg
     6841 ggctacctgg tgccgcaacc ggagtgcgtg acggtggaaa ccacgggcat tgacagcgaa
     6901 atcaccagtc aggcagggcc acagctggtg gttccggcaa tgaacgcccg ctacgcgctg
     6961 aacgcggcga acgctcgctg gggctcactg tacgatgcgt tatacggcag cgacatcatc
     7021 ccgcaggaag gggcgatggt cagcggctac gatccgcagc gcggtgagca ggttatcgcc
     7081 tgggttcggc gtttcctcga tgaatctcta ccgctggaaa acggcagcta tcaggatgtg
     7141 gtggcgtttc aggtggtcga taaacaatta cgcatccagt tgaaaaatgg taaagaaacc
     7201 acgttacgta ccccggcgca gtttgtcggt taccgtggcg atgccgctgc gccgacctgc
     7261 attttgctga aaaataacgg cctgcatatt gaactgcaaa ttgatgccaa cggacggatt
     7321 gggaaagacg atgcggcgca catcaacgat gttatcgtcg aagctgcaat cagtaccatt
     7381 ctcgactgcg aagattcggt cgcggcggtt gatgcggaag ataaaatcct gctgtaccgc
     7441 aatctgctgg gcctgatgca ggggactctc caagagaaaa tggagaaaaa cggtcgacaa
     7501 atcgtgcgta aactgaatga cgatcgtcag tacaccgccg ccgatggctc tgaaatttct
     7561 ctgcacggac gctcgctgct gtttatccgc aacgtgggtc atttgatgac cattcctgtg
     7621 atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc ttgatggcgt catgactggc
     7681 gcgattgccc tctatgattt aaaagtgcag aaaaactcgc gcactggcag cgtctatatt
     7741 gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg ccaacaaact gtttacccgc
     7801 attgagacaa tgctcggtat ggcaccgaat accctgaaaa tgggcattat ggatgaagaa
     7861 cgtcggacct cgctgaactt gcgtagctgt atcgctcagg cacgcaaccg cgtggcgttc
     7921 atcaataccg gtttcctcga ccgtaccggc gatgaaatgc attcggtgat ggaagctggc
     7981 ccgatgctgc gtaaaaatca gatgaaatcg acgccgtgga tcaaagccta cgagcgtaat
     8041 aacgtgcttt ccggcctgtt ctgtgggctg cgcggtaaag cgcaaatcgg taaaggcatg
     8101 tgggcaatgc cggacctgat ggcagacatg tacagccaga agggcgacca actgcgtgcc
     8161 ggggcaaaca cagcctgggt tccgtcgcca actgccgcta cgctccatgc gatgcactac
     8221 caccaaacca acgtacagag cgtgcaagcc aacattgccc agactgagtt caatgctgaa
     8281 tttgaaccgc tgctggacga tctgctgact attccggttg ctgaaaacgc taactggtcg
     8341 gcgcaagaga tccaacaaga gctggataac aacgtgcagg ggattctggg gtacgtggtg
     8401 cgctgggtgg agcaggggat tggttgttca aaagtgccgg atattcacaa tgtggcgctg
     8461 atggaagacc gcgcaacgct gcgtatctcc agccagcata tcgccaactg gttacgtcac
     8521 ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga atatggcgaa agtggttgat
     8581 cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg ggaatttcgc taactcgtgt
     8641 gcttttaaag ctgccagcga tttaatcttc ctcggcgtga aacagccaaa cggctatacc
     8701 gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa gtcattaata gcgtcaaaaa
     8761 aagccccggc aagttttatt gtcggggcta tgatagtaac tgcggtagta catttatccc
     8821 ggaaataacc ggagcgatta tcagggttta acaatattaa atacccgtag cgcattacta
     8881 gcgggcctat aacaacaata tggacatctt aacgatactc tgcgcctgcg tgtgcagaac
     8941 agaaaccctt acccgatata cttgttatgc cagaggtagc aattgcggca accgatctga
     9001 ctggcatgga tgcagatgca atggtggaag atataaatta cacgataatt acagatgttc
     9061 agattaccga acgaactaaa acctatgtca gagcagataa tgctaccact tttcggcagg
     9121 gaacatcagg caccgaaatt caggctagca cagaaaccag caaccaggtt aatctgaaat
     9181 ttgaaggggc taaacctctt ctggaaatac aactgaaata aatatcctcc ggctatgccg
     9241 gaggatattt attttaattt gaagcctgat ttacacgcag ctctgcgtag ggcagggtat
     9301 catagtcagt taacgcattt ttcttatagg ggtttcctat ccagcgagtg atttctttga
     9361 aatgctggtt taatatggaa cgctctggct catagttatc ataacgtaaa ccagccaaat
     9421 ccgaccaggt atggataaaa tcagccagac tgtatttacg gttaatgtac tcagaaaatg
     9481 tatgatgtcg attaagtagc catttttcag aggcccagag aataaatgga atattgtaca
     9541 taggtcgggt tggggtgtct tcgttacgtc ccagcatttt atgtggtggc gtatcgaata
     9601 cttcctctcc atggtcagaa aaatagaaaa gaaaaccatc aggggctgtt gctttgaaag
     9661 ttttgatcaa cgttgcaaca acatggtcat taaatatatt agcattatcg taatcgttat
     9721 ataattccag ttcattatca gtaagtcctt ctggtgcatg ttccctgtcc ccatcgaata
     9781 ctccctgacc ttcaggataa cggtatttat atttgatgtg tgttcccaac aagtgaataa
     9841 taacaaattt tttgggagct ggatctgcca gtacttcact aaacggagcc agtacgttag
     9901 tatcgtactc acgggcactt tgcgttctct gctgattcat gtagtactgt ttatctgttt
     9961 gccgagagaa gactgtcagc attgtgttgc gtgcagtcat cgtttgttga ttggtgatcc
    10021 agaatgtttt gtaaccggcc tgtttcatta tattcatcag ggaaggttgt gtcaggtaga
    10081 gttcgggctg ctgttcatca gcgaaagtca atacctgctg tagtacttcg atggtatatg
    10141 gtcttgagga gacaacatta ttaaacacaa acatattttg ttccgttttg tataattcat
    10201 ctagtccggg ggttgttttt ctgggatatc catacagact cattctccca cgctgggttg
    10261 attctcctat caccagaacc agtgttcgtg gggtatctcc ggaaatatcg ttaaagtttt
    10321 tcagagcggg gagcgcatta ttttcctcca gcaaacggtt cagagaatta agctggagac
    10381 ggtactgata atatccggta ataaattgcc agggggcagc gggctccata cgggatgcca
    10441 ggcgtcccag agtattgtca atagtcctgt ttttaacaac agtatttatt aatacagggt
    10501 gcagaattaa accgtagata ataaataaag gtataatgta gcgccagtat gatggtatat
    10561 atatcgggct aagacgagtc catagcagaa tagcaatagc tgtatatatt gtcgatataa
    10621 atagtatttt taaactaaaa tactgactaa aatattcgct ggcttcactt gtattacttt
    10681 caaacatgac gaacagtaca ctctgagaga attcctgccc gtagattaga taatagcaaa
    10741 gggcagcaag agatgtgccc cataaaacca cgccgattaa tgctgctgta acctttattc
    10801 tctctgggaa aagaaaaaca gggattaacc agagagagct gaaaagtagc gagtcacgaa
    10861 gtccattggt gccactatat ccagtcagac aaatgataat ctgaagtaaa gtggaaaaat
    10921 accagaaata aatgagagcc catcccaggg ctttccagct aaaaacatgt tcatgttttg
    10981 ttaatgtctt ctgcatatta ataaacctgc acgggaattt actattgtaa tttttatatg
    11041 agatgtatta aaacatttca tacagatttt ttgataagca catagtttca atattcacta
    11101 taatcgtttt ctgctgtgtt gtgtttgatc ctttttcaaa gaggcgctaa tcttccactt
    11161 gccaaggtta gatggtcagg gtccgggtta aacaaaatgt ttgacaggga gcctttccat
    11221 atgctgtctt tccagcgtgg cggtccgtaa tgtactaatt ttaagagagg tccgcaatct
    11281 tccagtggat ggttcatctg cctctctatt accgttacca tcgtcttgcg tcctgtgcta
    11341 tcgcctgtaa cgacatcagc gtcactggta ttaacaaact tatagttgaa gccagaactg
    11401 aaccgccccg gaaatcctgg agactaaact ccctgagaaa gaggtaaaca ggatgactaa
    11461 aaatactcgt ttttcccccg aagtccgtca gcgggcgatt cgtatggttc tggaaagtca
    11521 ggatgaatat gactcacagt gggcggcaat ttgttccatt gccccaaaga ttggctgtac
    11581 gccggagac
//
LOCUS       DAZFKM010000148        11582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFKM010000148 DAZFKM010000000
VERSION     DAZFKM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHKAY_004256"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHKAY_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO5702829.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHKAY_004257"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHKAY_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO5702830.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHKAY_004258"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHKAY_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO5702831.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHKAY_004259"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHKAY_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO5702832.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHKAY_004260"
     CDS             7014..7292
                     /locus_tag="ACHKAY_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702833.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHKAY_004261"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHKAY_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO5702834.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHKAY_004262"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHKAY_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO5702835.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHKAY_004263"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHKAY_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO5702836.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHKAY_004264"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHKAY_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO5702837.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /gene="yhiD"
                     /locus_tag="ACHKAY_004265"
     CDS             10284..10931
                     /gene="yhiD"
                     /locus_tag="ACHKAY_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO5702838.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(10973..11503)
                     /gene="dctR"
                     /locus_tag="ACHKAY_004266"
     CDS             complement(10973..11503)
                     /gene="dctR"
                     /locus_tag="ACHKAY_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO5702839.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT         3205 a         2582 c         2638 g         3157 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctatt ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtatcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcaattt gtaaatatca
     8041 aacaaaaagt taacgtttgc ggattactgg ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aatgatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc tggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     9121 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat agcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcag tttgccaaag gtaattaaca ggacagggct tataataata
    11581 at
//
LOCUS       DAZFKM010000149        11520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFKM010000149 DAZFKM010000000
VERSION     DAZFKM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            255..554
                     /gene="yoaC"
                     /locus_tag="ACHKAY_004267"
     CDS             255..554
                     /gene="yoaC"
                     /locus_tag="ACHKAY_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO5702840.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(674..853)
                     /gene="yoaH"
                     /locus_tag="ACHKAY_004268"
     CDS             complement(674..853)
                     /gene="yoaH"
                     /locus_tag="ACHKAY_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO5702841.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            927..2288
                     /gene="pabB"
                     /locus_tag="ACHKAY_004269"
     CDS             927..2288
                     /gene="pabB"
                     /locus_tag="ACHKAY_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO5702842.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2292..2870
                     /gene="nudL"
                     /locus_tag="ACHKAY_004270"
     CDS             2292..2870
                     /gene="nudL"
                     /locus_tag="ACHKAY_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO5702843.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3054..4418
                     /gene="sdaA"
                     /locus_tag="ACHKAY_004271"
     CDS             3054..4418
                     /gene="sdaA"
                     /locus_tag="ACHKAY_004271"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO5702844.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4549..6147
                     /gene="pdeD"
                     /locus_tag="ACHKAY_004272"
     CDS             4549..6147
                     /gene="pdeD"
                     /locus_tag="ACHKAY_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO5702845.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6151..7707)
                     /gene="yoaE"
                     /locus_tag="ACHKAY_004273"
     CDS             complement(6151..7707)
                     /gene="yoaE"
                     /locus_tag="ACHKAY_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO5702846.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7695..7853)
                     /gene="yoaL"
                     /locus_tag="ACHKAY_004274"
     CDS             complement(7695..7853)
                     /gene="yoaL"
                     /locus_tag="ACHKAY_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO5702847.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8170..9141
                     /gene="manX"
                     /locus_tag="ACHKAY_004275"
     CDS             8170..9141
                     /gene="manX"
                     /locus_tag="ACHKAY_004275"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO5702848.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9204..10004
                     /gene="manY"
                     /locus_tag="ACHKAY_004276"
     CDS             9204..10004
                     /gene="manY"
                     /locus_tag="ACHKAY_004276"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO5702849.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10017..10868
                     /gene="manZ"
                     /locus_tag="ACHKAY_004277"
     CDS             10017..10868
                     /gene="manZ"
                     /locus_tag="ACHKAY_004277"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO5702850.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10923..11381
                     /gene="yobD"
                     /locus_tag="ACHKAY_004278"
     CDS             10923..11381
                     /gene="yobD"
                     /locus_tag="ACHKAY_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO5702851.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2828 a         2893 c         2884 g         2915 t
ORIGIN      
        1 ccatgccgtt gtgttcgccc tctgactagc aactctggtt ttctttctta gctgaataat
       61 ctggttatag cgtgaggact gaccgttaaa tttctccatc ttactggata acacaccatg
      121 agctgctacc tttaaaacac ctgaatgtca acaggttaac tcgtgccata tcgttataat
      181 ccctttgata ttagatgcaa attaaggtca tatatagtct tatcgataac atggttaatt
      241 ttaaggataa gaatatgcct gctgtaatag ataaagccct ggatttcatt ggtgccatgg
      301 atgtatcagc gccaacacca agttcgatga atgaaagcac ggcgaaggga atctttaaat
      361 atttaaaaga actgggagta cccgcgagtg ccgctgatat tacggcgcga gctgaccagg
      421 aaggctggaa ccctgggttc acggaaaaaa tggttggatg ggcaaaaaaa atggagacag
      481 gtgaacgctc tgtgattaaa aatcctgaat acttttcaac atatatgcag gaagaactaa
      541 aagcactggt ctgagttaaa tttatatcag cataaatggg tcaggacgct tttaatcaca
      601 tataaaaagc gtcctttttc tcacaatcaa atgatcatcg gcttctataa cgaaatcgat
      661 acggattagt gtgttactcg tcttcatcct caaaacgcgc cacgattcgc tcgccggagt
      721 ggttggcacg cagctcttct gccaccagcg cgattgcctg gccgctgctc atcccttgtg
      781 ccatcagttc ctggatccgc tcgacagctt tttgctgctg ttcatgggtg agtgaaggta
      841 aacctgcaaa cattgttaac tcctgctaaa ttgttggcgc taattatttc atgctacccg
      901 gcacatagcc agtagagtca ggactgatga agacgttatc tcccgctgtg attactttac
      961 cctggcgtca ggacgccgct gaattttatt tctcccgctt aagccacctg ccgtgggcga
     1021 tgcttttaca ctccggctat gccgatcatc cgtatagccg ctttgatatt gtggtcgccg
     1081 atccgatttg cactttaacc actttcggta aagaaaccgt tgttagtgaa agcgaaaaac
     1141 gcacaacgac cactgatgac ccgctacagg tgctccagca ggtgctggat cgcgcagaca
     1201 ttcgcccaac gcataacgaa gatttgccat ttcagggcgg cgcactgggg ttgtttggct
     1261 acgatctggg ccgccgtttt gagtcactgc cagaaattgc ggaacaagat atcgttctgc
     1321 cggatatggc agtgggtatc tacgattggg cgctcattgt cgaccaccag cgtcatacag
     1381 tttctttgct gagtcataat gatgtcaatg cccgtcgggc ctggctggaa agccagcaat
     1441 tctcgccgca ggaagatttc acgctcactt ccgactggca atccaatatg acccgcgagc
     1501 agtacggcga aaaatttcgc caggtacagg aatatctgca cagcggtgat tgctatcagg
     1561 tgaatctcgc ccagcgtttt catgcgacct attctggcga tgaatggcag gcattccttc
     1621 agcttaatca ggccaaccgc gcgccattta gcgctttttt acgtcttgaa cagggtgcaa
     1681 ttttaagcct ttcgccagag cggtttattc tttgtgataa tagtgaaatc cagacccgcc
     1741 cgattaaagg cacgctacca cgcctgcccg atcctcagga agatagcaaa caagcagaaa
     1801 aactggcgaa ctcagcgaaa gatcgtgccg aaaatctgat gattgtcgat ttaatgcgta
     1861 atgatatcgg tcgtgttgcc gtagccggtt cggtaaaagt accagagctc ttcgtggtgg
     1921 aacccttccc tgccgtgcat catctggtca gcactataac ggcgcgacta ccagaacagt
     1981 tacacgccag cgatctgctg cgcgcagctt ttcctggtgg ctcaataacc ggggctccga
     2041 aagtacgggc tatggaaatt atcgacgaac tggaaccgca gcgacgtaat gcctggtgcg
     2101 gcagcattgg ctatttgagc ttttgcggca acatggatac cagcattact atccgcacgc
     2161 tgactgccat taacggacaa atatactgct ctgcgggcgg tggaattgtc gccgatagcc
     2221 aggaagaagc ggaatatcag gaaacttttg ataaagttaa taagatatta cgccaactgg
     2281 agaagtaaga cgtggaatac cgtagcctga cgcttgatga ttttttatct cgctttcaac
     2341 ttttgcgccc gcaaattaac cgggaaaccc taaatcatcg tcaggctgcc gtgttaatcc
     2401 ccatcgtccg tcgaccgcaa ccggggttgt tgctgactca gcgttcaatt catctgcgta
     2461 aacacgctgg acaagtggca ttccctggag gtgcagtcga tgacacggac gcatcagtta
     2521 tcgccgccgc gctgcgcgaa gctgaagaag aggtcgctat accgccttcc gccgttgaag
     2581 ttatcggcgt gctgccgccc gtcgatagcg taactggcta tcaggtaacc ccagtggtcg
     2641 gcattatccc gcccgatctg ccgtatcgcg ccagtgaaga tgaagtctcg gcggtgtttg
     2701 aaatgccgct cgcccaggca ttacatctgg gtcgttatca ccctttagat atctaccgcc
     2761 gtggcgattc acatcgggta tggctgtcct ggtacgaaca gtattttgta tggggaatga
     2821 ccgcaggcat aattcgtgag ctggcgctgc aaattggtgt gaaaccctga ctatacttat
     2881 ctttacatct acaaaacact acttgagaca atcatcgcaa tattagttaa atcgcggttt
     2941 ttgattagtt taattcatgt gaatagttaa gccagtcgcc gcgttccctc ttacactatg
     3001 cgctgttatt agttcgttac tggaagtcca gtcaccttgt caggagtatt atcgtgatta
     3061 gtctattcga catgtttaag gtggggattg gtccctcatc ttcccatacc gtagggccta
     3121 tgaaggcggg taaacagttc gtcgatgatc tggtcgaaaa aggcttactg gatagcgtta
     3181 ctcgcgttgc cgtggacgtt tatggttcac tgtcgctgac gggtaaaggc caccacaccg
     3241 atatcgccat tattatgggt cttgcaggta acgaacctgc caccgtggat atcgacagta
     3301 ttcccggttt tattcgcgac gtagaagagc gcgaacgtct gctgctggca cagggacggc
     3361 atgaagtgga tttcccgcgc gacaacggga tgcgttttca taacggcaac ctgccgctgc
     3421 atgaaaacgg tatgcaaatc cacgcctata acggcgatga agtcgtctac agcaaaactt
     3481 attattccat cggcggcggt tttatcgtcg atgaagaaca ctttggtcag gatgctgcca
     3541 acgaagtgag cgtgccgtat ccgttcaaat ctgccaccga actgctcgcg tactgtaatg
     3601 aaaccggcta ttcgctgtct ggtctcgcta tgcagaacga actggcgctg cacagcaaga
     3661 aagagatcga cgagtatttc gcgcatgtct ggcaaaccat gcaggcatgt atcgatcgcg
     3721 ggatgaacac cgaaggtgta ctgccaggcc cgctgcgcgt gccacgtcgt gcgtctgccc
     3781 tgcgccggat gttagtttcc agcgataaac tgtctaacga tccgatgaat gtcattgact
     3841 gggtaaacat gtttgcgctg gcagttaacg aagaaaacgc cgccggtggt cgtgtggtaa
     3901 ctgcgccaac caacggtgcc tgcggtatcg ttccggcagt gctggcttac tatgaccact
     3961 ttattgaatc ggtcagcccg gacatctata cccgttactt tatggcagcg ggcgcgattg
     4021 gtgcattgta taaaatgaac gcctctattt ccggtgcgga agttggttgc cagggcgaag
     4081 tgggtgttgc ctgttcaatg gctgctgcgg gtcttgcaga actgctgggc ggtagcccgg
     4141 aacaggtttg cgtggcggcg gaaattggca tggaacacaa ccttggttta acctgcgacc
     4201 cggttgcagg gcaggttcag gtgccgtgca ttgagcgtaa tgccattgcc tctgtgaagg
     4261 cgattaacgc cgcacggatg gctctgcgcc gcaccagtgc accgcgcgtc tcgctggata
     4321 aggtcatcga aacgatgtac gaaaccggta aggacatgaa cgccaaatac cgcgaaacct
     4381 cacgcggtgg tctggcaatc aaagtccagt gtgactaata cttcttactc gcccatctgc
     4441 aacggatggg cgaatttata cccgctttct cgtctgctgt aatattcccc actacacttc
     4501 cactgttgcg tcaggcgttt gtcgccatac gcttacaggg tggcccgcat gcaaaaagca
     4561 caacggatca ttaaaaccta tcgccgtaat cgaatgattg tttgtacgat ttgcgcactc
     4621 gttacgctcg cttcgaccct gagcgtgcga tttatttcac agcgtaactt aaatcaacaa
     4681 cgggtagtac aattcgccaa tcacgctgta gaggaattag ataaagtact gcttccccta
     4741 caggcaggta gcgaagtctt gcttccgctg attggtctgc cctgctctgt cgcccatttg
     4801 ccattacgta aacaggcggc aaaactccaa actgtgcgat ccattggcct ggtgcaagac
     4861 ggcacactct attgctccag catttttggt tatcgcaatg tgcccgtcgt ggacattctg
     4921 gctgaacttc ctgcaccgca accactttta cgcctgacga tcgaccgtgc cctgattaaa
     4981 ggcagtccgg ttttgattca atggacgcct gcagcgggca gtagcaatgc tggggtcatg
     5041 gagatgatta acatcgactt actgacggca atgctccttg agccacaact gccgcaaatc
     5101 agtagcgcca gcctgacggt ggacaaacgg catttgctct atggtaatgg gctggtagat
     5161 tcccttccgc aacctgaaga caatgaaaac tatcaggttt cttcgcaacg ctttcctttt
     5221 accattaacg ttaatggtcc gggggctacg gcgctggcat ggcactatct tccaacacaa
     5281 ttaccgctgg cggtgctgct aagtttactg gtgggctaca tcgcctggct ggcgaccgct
     5341 taccggatga gcttttcccg cgaaatcaat ctgggcctgg cgcaacatga gttcgaattg
     5401 ttctgtcagc ctttgcttaa tgcgcgcagc cagcaatgta ttggtgtaga gattttgctg
     5461 cgctggaata atccgcgtca gggctggatt tcaccggatg tgtttattcc tatcgcggaa
     5521 gaacatcatt taattgtgcc actgacccgc tatgtgatgg cagaaaccat tcgtcagcgc
     5581 catgttttcc cgatgagtag tcagtttcat gttggcatta acgtcgcacc cagccatttt
     5641 cgccgtggtg tgctgataaa agatctcaat cagtactggt ttagcgctca cccgattcag
     5701 caactgatcc tcgaaatcac cgaacgcgat gccttactgg atgttgatta tcggattgcc
     5761 cgcgagctac atcgtaaaaa cgtcaaactg gcgattgatg acttcggcac cggcaacagt
     5821 tcattttcct ggcttgaaac attacgtcct gacgtgctga aaattgataa gtcatttacc
     5881 gcagctatag gttctgacgc ggttaactcg acggtgaccg atatcatcat cgcgctgggg
     5941 caaagactga atattgaact ggtggcggag ggcgtggaaa cgcaagaaca ggcgaagtat
     6001 ttgcgccgtc atggcgtgca tattttgcaa gggtatttgt acgcacagcc gatgccgcta
     6061 cgtgattttc ccaaatggct ggcgggcagc caaccgccgc ccgcccggca taatggacat
     6121 atcacgccca ttatgccgtt acgttaatgc ttactcatct tcatcgtgcg ccggttgctc
     6181 tttaacaatg cgaaccagat caacacgata atcattggct tcaatgatgg tgatatgcag
     6241 tggccctaca tcaatcacat cgcccacacg gggaatgtga ccatttgccg agatcacgag
     6301 gcccgcgacc gtcgcgatat catcgtcatc ggcaaggtgc tcaacatcaa gcgcctgctg
     6361 caaggcatgc aaatctgtac cgccttttac cagccagccg tcaccatcag taatgatttc
     6421 cggcgtttcg tcagcgtccg ggaattcacc cgcaatggct tccagcacat ccagcggcgt
     6481 gaccagacct tgtaccacac caaactcgtt ggtcacgata acaaagctcc cgcgagcacg
     6541 acgcagcacg cccaacaggt tgatcggatc gagggtttcc gggacgataa tcgccggaga
     6601 cgccgaagca atcgccgcca catcaacgcc ctcttccagc gccaccagca gttctttagc
     6661 acgtacaata ccgatgattt catccagttc accgcgacat accgggaaca gactgtgcgg
     6721 tgaagagagc agttgctcgc ggatttcatc gaccccgaga ttagcgtcaa cccagcttat
     6781 ttcaccgcgc ggcgtcatga tcccgcgcag agaacgcgac gccagcgtca gtacgccgtt
     6841 aatcatgtaa cgttcttctt cggcaaatgc accttccggg atcggcatcg gcatcgggtt
     6901 atcggcatcg tgctgaacat tggcctgacg tttcccgccc atcaaacgca ggatggcatc
     6961 ggcagtacgc gctcgcagcg gcaaagtcga ctggtggcga ataaagttgc gacgcgcaat
     7021 ctggttaaac acttcgatga tgatcgagaa gccaatcgcg gcatacaggt aacctttcgg
     7081 aatgtggaaa ccgaaacctt ctgccaccag actcagacca atcattaaca ggaagctcag
     7141 acagagcacc accaccgtgg ggtgctggtt aacgaatcgc gtcagcggtt tggatgccag
     7201 caacataacc gccatcgcaa tcactaccgc cgccatcatc accggcagat ggttaaccat
     7261 ccctactgca gtaattaccg catccaacga gaagacggcg tcaaggatga caatctgtgt
     7321 gacgaccacc cagaaactgg cgtagccttt accgtggccg gaatcatgat cgcggttttc
     7381 cagccgttca tgcagttcgg ttgttgcttt gaacagcaag aatatccccc cgaacaacat
     7441 aatcaggtcg cgtccggaga aggagaaatc catgacggta aatagcggtt tggtcagcgt
     7501 gaccatccat gaaatcagcg acagcagccc cagacgcata atcagcgcca gtgataaccc
     7561 cagcaaacgc gctttatcgc gttgttttgg cggcagtttg tcagcaagaa tggcgatgaa
     7621 gaccaggtta tcgataccca gcacaatttc gagaacaaca agcgtgagta gccccgccca
     7681 aattgagggg tccattaaga attccatgac aagctcctgc ttaaggaata gctattcgac
     7741 gccagaaata atgcaggcgt aacgacaaaa tgcaaacgaa aggtgcggca tagagagcca
     7801 gaaaggtagg cgttaaaagg cctgatgctg aaatgacgtc ggtgacgatc catactgcgg
     7861 gctactgccc tatactccat ggttgttaaa cgggagttaa acatatcaga gacgcctctg
     7921 atttggcaaa gatttacctt cctttgcaaa cgaatgtgac aaggatattt tacctttcga
     7981 aatttctgct aatcgaaagt taaattacgg atcttcatca cataaaataa ttttttcgat
     8041 atctaaaata aatcgcgaaa cgcaggggtt tttggttgta gcccttatct gaatcgattc
     8101 gattgtggac gacgattcaa aaatacatct ggcacgttga ggtgttaacg ataataaagg
     8161 aggtagcaag tgaccattgc tattgttata ggcacacatg gttgggctgc agagcagttg
     8221 cttaaaacgg cagaaatgct gttaggcgag caggaaaacg tcggctggat cgatttcgtt
     8281 ccaggtgaaa atgccgaaac gctgattgaa aagtacaacg ctcagttggc aaaactcgac
     8341 accactaaag gcgtgctgtt tctcgttgat acatggggag gcagcccgtt caatgctgcc
     8401 agccgcattg tcgtcgacaa agagcattat gaagtcattg caggcgttaa cattccaatg
     8461 ctcgtggaaa cgttaatggc ccgtgatgat gacccaagct ttgatgaact ggtggcactg
     8521 gcagtagaaa caggccgtga aggcgtgaaa gctctgaaag ccaaaccggt tgaaaaagcc
     8581 gcgcaagcac ccgctgccgc agcaccaaaa gcggctccaa ctccggcaaa accaatgggg
     8641 ccaaacgact acatggttat tggccttgcg cgtatcgacg accgtctgat tcacggtcag
     8701 gtcgccaccc gctggaccaa agaaaccaat gtctcccgta ttattgttgt tagtgatgaa
     8761 gtggctgcgg ataccgttcg taagacactg ctcacccagg ttgcacctcc gggcgtaaca
     8821 gcacacgtag ttgatgttgc caaaatgatt cgcgtctaca acaacccgaa atatgctggc
     8881 gaacgcgtaa tgctgttatt taccaaccca acagatgtag agcgtctcgt tgaaggcggc
     8941 gtgaaaatca cctctgttaa cgtcggtggt atggcattcc gtcagggtaa aacccaggtg
     9001 aataacgcgg tttcggttga tgaaaaagat atcgaggcgt tcaagaaact gaatgcgcgc
     9061 ggtattgagc tggaagtccg taaggtttcc accgatccga aactgaaaat gatggatctg
     9121 atcagcaaaa tcgataagta acgtattgtg ttgattatca ctcagttttc acacttaagt
     9181 cttacgtaaa caggagaagt acaatggaga ttaccactct tcaaattgtg ctggtattta
     9241 tcgtagcctg tatcgcaggt atgggatcaa tcctcgatga atttcagttt caccgtccgc
     9301 taatcgcgtg taccctggtg ggtatcgttc ttggggatat gaaaaccggt attattatcg
     9361 gtggtacgct ggaaatgatc gcgctgggct ggatgaacat cggtgctgca gttgcgcctg
     9421 acgccgctct ggcttctatc atttctacca ttctggttat cgcaggtcat cagagcattg
     9481 gtgcaggtat cgcactggca atccctctgg ccgctgcggg ccaggtactg accatcatcg
     9541 ttcgtactat taccgttgct ttccagcacg ctgcggataa ggctgctgat aacggcaacc
     9601 tgacagcgat ttcctggatc cacgtttctt ctctgttcct gcaagcaatg cgtgtggcta
     9661 ttccggccgt catcgttgcg ctgtctgttg gtaccagcga agtacagaac atgctgaatg
     9721 cgattccgga agtggtgacc aatggtctga atatcgccgg tggcatgatc gtggtggttg
     9781 gttatgcgat ggttatcaac atgatgcgtg ctggctacct gatgccgttc ttctacctcg
     9841 gcttcgtaac cgcagcattc accaacttta acctggttgc tctgggtgtg attggtactg
     9901 ttatggcagt gctctacatc caacttagcc cgaaatacaa ccgcgtagcc ggtgcgcctg
     9961 ctcaggcagc tggtaacaac gatctcgata acgaactgga ctaacaggtg agcgaaatgg
    10021 ttgatacaac tcaaactacc accgagaaaa aactcactca aagtgatatt cgtggcgtct
    10081 tcctgcgttc taacctcttc cagggttcat ggaacttcga acgtatgcag gcactgggtt
    10141 tctgcttctc tatggtaccg gcaattcgtc gcctctaccc tgagaacaac gaagctcgta
    10201 aacaagctat tcgccgtcac ctggagttct ttaacaccca gccgttcgtg gctgcgccga
    10261 ttctcggcgt aaccctggcg ctggaagaac agcgtgctaa tggcgcagag atcgacgacg
    10321 gtgctatcaa cggtatcaaa gtcggtttga tggggccgct ggctggtgta ggcgacccga
    10381 tcttctgggg aaccgtacgt ccggtatttg cagcactggg tgccggtatc gcgatgagcg
    10441 gcagcctgtt aggtccgctg ctgttcttca tcctgtttaa cctggtgcgt ctggcaaccc
    10501 gttactacgg cgtagcgtat ggttactcca aaggtatcga tatcgttaaa gatatgggtg
    10561 gtggcttcct gcaaaaactg acggaagggg cgtctatcct cggcctgttt gtcatggggg
    10621 cattggttaa caagtggaca catgtcaaca tcccgctggt tgtctctcgc attactgacc
    10681 agacgggcaa agaacacgtt actactgtcc agactattct ggaccagtta atgccaggcc
    10741 tggtaccact gctgctgacc tttgcttgta tgtggctact gcgcaaaaaa gttaacccgc
    10801 tgtggatcat cgttggcttc ttcgtcatcg gtatcgctgg ttacgcttgc ggcctgctgg
    10861 gactgtaaga ctgttgtaca ctaccggggc cttttggccc cgttttttta tctggaggat
    10921 taatgacaat cacggacctg gtactgattc ttttcatcgc cgcactcctg gccttcgcga
    10981 tctacgatca gttcatcatg ccccgccgta acggccccac cctgctggca attcctttgc
    11041 tccggcgtgg tcgcatcgat agcgttatct tcgtcggatt gattgtcatt cttatctata
    11101 acaacgtcac gaatcatggt gcgttaataa cgacatggtt attaagcgca ctggctctga
    11161 tgggttttta tatattctgg atccgcgttc cgaagatcat ctttaaacaa aaaggttttt
    11221 tcttcgccaa tgtctggatt gaatatagcc gaatcaaagc gatgaacttg tcggaagatg
    11281 gcgtgttggt gatgcaatta gaacagcgtc ggctgttaat ccgcgttcga aatatcgacg
    11341 atctggaaaa aatttataag cttctcgttt caactcaata agttatgaat ttagccaaag
    11401 ctatgtttag tgtattttta ataatcagac atagcttagg ctatattacc tcttccctta
    11461 tttgttattt attttaacgt ttcattgata tataaatcta aatgtaaatc gttatcaata
//
LOCUS       DAZFKM010000150        11381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFKM010000150 DAZFKM010000000
VERSION     DAZFKM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..926
                     /locus_tag="ACHKAY_004279"
     CDS             <1..926
                     /locus_tag="ACHKAY_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO5702852.1"
                     /translation="DTIARVVSCISPAKFHECFINWMRDCHSSNDKDVIAIDGKTLRH
                     SYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDA
                     MGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEGKFPLKELNNPEHDSYAISEKSHG
                     REEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSAD
                     LTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTND
                     KVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1195..3432
                     /gene="nrfB"
                     /locus_tag="ACHKAY_004280"
     CDS             1195..3432
                     /gene="nrfB"
                     /locus_tag="ACHKAY_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO5702853.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAGLAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3419..6391
                     /gene="nfrA"
                     /locus_tag="ACHKAY_004281"
     CDS             3419..6391
                     /gene="nfrA"
                     /locus_tag="ACHKAY_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO5702854.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6392..7282
                     /locus_tag="ACHKAY_004282"
     CDS             6392..7282
                     /locus_tag="ACHKAY_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO5702855.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7465..8226
                     /gene="envY"
                     /locus_tag="ACHKAY_004283"
     CDS             7465..8226
                     /gene="envY"
                     /locus_tag="ACHKAY_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO5702856.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8739..9692
                     /gene="ompT"
                     /locus_tag="ACHKAY_004284"
     CDS             8739..9692
                     /gene="ompT"
                     /locus_tag="ACHKAY_004284"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO5702857.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9879..11363
                     /locus_tag="ACHKAY_004285"
     CDS             9879..11363
                     /locus_tag="ACHKAY_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5702858.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         3110 a         2789 c         2744 g         2738 t
ORIGIN      
        1 acgatactat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac gagtgcttta
       61 ttaactggat gcgtgactgc cattcatcaa atgataaaga cgtcattgca attgatggaa
      121 aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat gtcattagtg
      181 cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag aaatctaatg
      241 agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa atcatcacaa
      301 ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag ggaggtgatt
      361 atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag ggaaaatttc
      421 cgctgaaaga attaaataat ccagagcatg acagttacgc aatcagtgaa aagagtcacg
      481 gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt attgatttca
      541 cgtttgaatg gaaaggtctg aagaaattat gcgtggcagt ctcctttcgg tcaataatag
      601 cagaacaaaa gaaagagccc gaaatgacgg tcagatatta tatcagttct gctgatttaa
      661 ccgcagagaa gttcgccaca gcaatccgaa accactggca cgtggagaat aagctgcact
      721 ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga aacgccgcag
      781 aattattttc agggatacgg cacatcgcta ttaatatttt aacgaatgat aaggtattca
      841 aggcagggtt aagacgtaag atgcgaaaag cagccatgga cagaaactat ctggcgtcag
      901 tccttgcggg gagcgggctt tcgtaatctt gccctgcctg ctcacctcct ccaggcactt
      961 ccactacatt atgacctgct cggtgccacc gctccttttc actatgacgt taacctcccc
     1021 tccttgacac catgcttttg tctacattct acgcaaacgg caacttttat aaccagtgta
     1081 aaaataacgt gccgcaatag tcatacctat taatggtaaa aagctgtcac aattcataaa
     1141 aaaccttaat atacgccacc ctaaacataa ccagcgttaa tgtaaggttt ttgtgtggac
     1201 tggcttcttg atgtttttgc aacctggctc tacggcttaa aagtaatcgc gataacgtta
     1261 gcggtcatca tgttcatcag cgggctggac gattttttta ttgatgtcgt ctactgggta
     1321 cgccgcatta aacgcaagtt gagtgtttat cgccgctacc cgcgaatgag ttaccgcgaa
     1381 ctgtataaac cagatgaaaa accgttagcg attatggttc cggcgtggaa tgaaacgggc
     1441 gtcatcggca atatggccgg gctggcggcg accacgctcg actacgaaaa ctatcatatc
     1501 tttgttggca cctaccccaa cgaccccgat actcaacgtg atgttgacga agtgtgcgct
     1561 cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg gtcccaccag caaagccgac
     1621 tgtctgaaca acgtgctgga cgccatcacc cagtttgaac gtagcgccaa tttcgctttt
     1681 gctggtttta ttctgcatga cgccgaagat gtgatttcac cgatggaatt gcgtctgttc
     1741 aactatctgg tcgagcgtaa agatctgatt cagatcccgg tgtatccgtt cgaacgcgaa
     1801 tggacgcact tcaccagcat gacttacatt gatgagtttt cagagctgca tggcaaagat
     1861 gttccggtgc gtgaagccct cgccggacaa gtgcccagcg caggcgtcgg cacctgtttc
     1921 agccgccgcg ccgtgaccgc tctgttagct gacggtgacg gtattgcttt cgacgtgcag
     1981 agtctgactg aagattacga cattggtttc cgcctgaaag agaaaggtat gacggaaatt
     2041 tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg agcagcgtaa atttttacag
     2101 cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt tccccgatac cttttcgact
     2161 gcagttcgac aaaaatctcg ctggatcatc ggcattgttt tccagggctt taaaacccac
     2221 aaatggacct ccagcctgac gctgaactac tttctctggc gcgaccgcaa aggggcaatc
     2281 agtaactttg tcagcttcct cgcgatgctg gtgatgctcc agcttttgct gttgctggcg
     2341 tatgaaagtt tgtggcccga tgcctggcat ttcctttcta tttttagcgg cagcgcatgg
     2401 ttaatgaccc tgctgtggct aaactttggc ttgatgttta accgcatcgt gcagcgggtg
     2461 attttcgtca ctggctacta cggtctgacg caggggctgc tatctgtcct gcgtcttttc
     2521 tggggcaacc tgattaactt catggccaac tggcgcgcgc taaaacaggt acttcaacac
     2581 ggcgatccac gtcgcgtcgc gtgggataaa acaacgcatg acttccccag cgtgactggc
     2641 gatacccgct cgttgcgccc gttaggccaa attctgctgg aaaatcaggt catcactgaa
     2701 gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc tacgcctggg cggttcaatg
     2761 ctgatgcagg ggctgattag cgccgagcag ctggcacagg cgctggcaga gcaaaacggc
     2821 gtggcgtggg aatccatcga tgcctggcag atcccgtcct cgctgattgc cgaaatgcca
     2881 gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc tggaaaatga tgagttaatt
     2941 gtcggcagtg aagatggtat tgaccctgtt tcgctggcgg ccctgacgcg taaagtcgga
     3001 cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg tcacggggtt acgtcactgg
     3061 tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt acaatgcggt tcagcatcag
     3121 tggctcacgg aacagcagac cggtgaaatc tggcggcaat atgtgccgca tcagttcctg
     3181 ttcgccgaaa tactgaccac gctcggtcat attaatcgtt cagcaattaa cgtgttgtta
     3241 ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt tggtcaccga aggcgttatc
     3301 agccaggaaa cgttggatcg cgtcctgaca attcaacgcg aattacaagt ttcgatgcaa
     3361 tcactattac tcaaagcagg tttaaacaca gaacaggttg cgcaactgga gtccgaaaat
     3421 gaaggagaat aaccttaatc gcgtcatcgg atggtctggt ttactgctga cgtctttatt
     3481 gagtaccagc gcactcgcag acaatatcgg caccagcgca gaagagctgg ggctgagcga
     3541 ttatcgccat tttgttattt atccccgtct cgataaggcg ctgaaggcac agaaaaataa
     3601 cgacgaagca accgccatcc gcgaatttga atatatacac cagcaggtgc cggataatat
     3661 tccgctgact ttataccttg cggaagccta tcgccatttt ggtcatgatg accgggcgcg
     3721 gctgttgctg gaggatcaac tgaaacgtca cccaggagat gcccgacttg agcgcagtct
     3781 ggcggctatt ccagttgaag tgaaaagcgt tacgactgtt gaagaactgc ttgcccagca
     3841 aaaagcgtgc gatgctgcgc cgaccctgcg ttgtcgcagt gaagtcgggc agaatgccct
     3901 gcggctggca cagttacctg tcgccagagc gcaactgaac gatgcgacgt ttgctgcatc
     3961 gccggaagga aaaacgctgc gaaccgatct gctgcaacgg gcaatctacc tgaaacaatg
     4021 gtcccaggca gatacgctat acaatgaagc acgccagcag aacacattaa gcgcggcaga
     4081 acgccgtcag tggtttgacg tgcttcttgc cgggcagctg gacgatcgga tcctggcact
     4141 gcaatcacag gggatcttca ccgatcctca gtcatatatt acttacgcga ccgcgctggc
     4201 ttatcgtggc gaaaaagcac gcctccagca ttatctcatt gaaaataagc cgctgtttac
     4261 cacggacgca caagagaaaa gttggctcta tctgttatct aaatacagcg ccaaccccgt
     4321 tcaggcattg gcgaattata cggtgcagtt tgccgataac cgccagtatg ttgttggcgc
     4381 gacgctaccg gtgctgttaa aagaaggtca gtacgacgca gcgcaaaaac tgctcgccac
     4441 cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc agcgtggcga cccgtaacaa
     4501 ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa gaaccggcaa atcttacccg
     4561 cctagatcaa ctgacctggc aactgatgca gaacgagcag tcacgcgaag ctgccgattt
     4621 attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc agccagactt taatggcgcg
     4681 actggcgtct ctgctggaaa gtcatcctta cctggcaact ccagcgaagg tggcgatttt
     4741 atcaaaaccc ttgccgctgg cggcgcaacg tcagtggcaa agccagttgc cgggtattgc
     4801 agataattgc ccggcaatag ttcgcttgct gggcgatatg tcgccttcct acgatgccgc
     4861 cgcctggaac cgtctggcaa agtgttatcg ggacacgcta cccggtgtgg ccttgtatgc
     4921 atggcttcag gccgaacaac gacaaccgaa cgcctggcaa catcgtgcgg tagcctatca
     4981 ggcgtatcag gttgaggact acgccaccgc actggcggcc tggcagaaaa tcagtcttca
     5041 cgacatgagc aatgaggatc tgcttgctgc tgccaatacc gcccaggcgg caggaaatgg
     5101 tgcagctcgc gatcgctggc tacaacaggc agaacaacgt ggactgggaa acaatgccct
     5161 ctactggtgg ctgcatgcgc aacgttacat tcctggtcag ccggaactcg cactgagcga
     5221 tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct tacgttgcgc gggcgacaat
     5281 ttatcgccaa cgtcataatg tcccggcggc ggtgagtgat ttgcgcgccg cgctggaact
     5341 ggaaccgaat aatagcaaca tccaggcagc gcttggttac gccttgtggg atagcggtga
     5401 tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa gggcttccgg acgatccggc
     5461 actgatccga caactggcct acgtgaacca gcgtctggat gacatgcctg cgacgcagca
     5521 ctacgcccgg ctggtgattg atgacattga taatcaggcg ctgataaccc cactgacccc
     5581 agagcaaaat cagcaacgct tcaatttccg ccgtttgcat gaggaggtcg gtcgccgctg
     5641 gacgttcagt ttcgattctt ctatcggctt gcgttccggg gcaatgagca ccgctaacaa
     5701 taatgtcggc ggcgcagcgc cagggaaaag ctatcgtagc tacgggcaac tggaagccga
     5761 gtaccgcatc ggacgcaata tgctgctgga aggcgacctg ctctcagttt atagccgcgt
     5821 ctttgccgat accggagaaa acggggtgat gatgccggtg aaaaatccga tgtccggcac
     5881 cggtctgcgc tggaagccgc tgcgcgatca gatctttttc ctcgccgtcg aacagcagtt
     5941 gccgctgaac ggccaaaatg gcgcatccga taccatgctg cgcgccagcg cctcattctt
     6001 taatggcggc aaatacagcg acgaatggca cccgaacggt tcaggctggt ttgcccaaaa
     6061 cctgtacctc gatgcggcgc aatatatccg ccaggatatt caggcgtgga cggcagatta
     6121 tcgcgtcagc tggcatcaga aggtagctaa cggacagact attgagcctt acgctcacgt
     6181 tcaggacaac ggctatcgtg ataaaggcac tcagggcgcg cagcttggcg gagtcggggt
     6241 ccgctggaat atctggaccg gcgagacgca ctacgacgcc tggccgcaca aagtcagtct
     6301 cggcgtcgag tatcaacata cctttaaggc gattaatcaa cgtaacggag agcgcaacaa
     6361 cgcgtttctc accattggag tgcactggta aatgcgtaag ttcattttcg tattgctgac
     6421 actgcttttg gtcagccctt tttcctttgc gatgaaaggt attatctggc aaccacaaaa
     6481 ccgagatagt caggttaccg atacccagtg gcaggggctg atgagtcagt tacgtttgca
     6541 aggcttcgat accctggttt tgcaatggac ccgttacggc gatgcattca cccagccaga
     6601 acagcgcgcg ttattgttta agcgtgccgc agctgcgcaa caggctggcc tgaagctgat
     6661 tgtcgggctg aacgccgatc cggaattttt tatgcaccag aaacagtcgt ccgcagcgct
     6721 ggaaagctat cttaatcgcc tgctggctgc cgatctccag caagccagat tatggagcgc
     6781 cgcgcctggc gtaacgccgg atggctggta catcagcgcg gaaattgacg acctgaactg
     6841 gcgcagcgaa gctgcccgtc agcctttgct aacatggtta aacaacgcgc agcggctgat
     6901 tagcgatgtt tcagcaaaac cggtttatat cagtagtttt ttcgccggaa acatgtcgcc
     6961 cgatggctat cgccaactgc tggaacaagt taaagcaacc ggcgttaatg tctgggtaca
     7021 ggatggcagc ggcgtggata aacttaccgc tgaacagcgt gaacgttatc tacaggccag
     7081 cgccgattgc caaagtcccg cccctgccag cggcattgtt tatgaactgt ttgtcgccgg
     7141 caaaggcaaa acctttacag cgaaaccgaa accggatgca gagatcgcct cgctgttagc
     7201 gaaacgttcc tcttgcggta aagacactct ctatttctct ctgcgctatt tgcccgtcgc
     7261 gcacggcatt ctcgagtatt aaatctcctc caggtaagtc gggtacgacc tggcttacct
     7321 ctttcgctct cgtcagaatt ccatcaagat taattgctaa aaagcgtgct aaagaaagaa
     7381 gatcgttatc ggcgaacggg ccgatcactc atgcaggaag catagatcct ttgagcggag
     7441 tttctgatat ctgaggagtg cgaaatgcaa ttgagcagca gtgaaccttg cgtggtgatc
     7501 ctgaccgaaa aagaggtaga ggtaagcgtc aataaccatg ctacgtttac ccttccgaaa
     7561 aactacctgg ccgccttcgc gtgcaacaat aacgtcattg aactctcaac gttaaatcac
     7621 gtattaatcc cccacatcaa ccgtaacatc atcaacgatt atctgttgtt tttaaataag
     7681 aacttaacct gtgtaaagcc ctggtcgcgg ctggcaaccc cggttatcgc ttgtcatagc
     7741 cgtacaccgg aagtgttccg gctagccgcc aaccacagca agcagcaacc cagcaaaccc
     7801 tgcgaagcgg agttgacgcg cgcattactt tttaccgtat tgtctaactt tcttgagcaa
     7861 tcgcggttta tagccctact gatgtatatc ttacgcagca gcgtccgcga cagcgtctgc
     7921 cgcattattc aaagcgatat tcagcattac tggaatctgc gaattgtcgc cagttcgcta
     7981 tgtttaagcc ccagcctact caaaaagaaa ttaaaaaacg aaaataccag ctatagccag
     8041 attgtcacag agtgtcgtat gcgttacgcc gtacagatgt tattgatgga taacaaaaat
     8101 atcactcagg tggcgcaatt atgtggctat agcagcacgt cgtactttat ctctgttttt
     8161 aaggcgtttt acggcctgac accgttgaat tatctcgcca aacagcgaca aaaagtgatg
     8221 tggtgaaggg caaagcggaa acggataaga cgggcataaa tgaagaagaa atggcgcgcc
     8281 ctgcaggatt cgaacctgcg gcccacgact tagaagttcc tagaacgaca ttttaagtca
     8341 acaaattacc gcgccatctc tgcgctcaca cgtcccacta cctcaaaaca tgtaaagcct
     8401 tgcaagccat tgtgaggcct tatgtgtctc agttttgtcc ctcttttttg tactaaaaaa
     8461 catagcaatt gaggataaac ctcatgctat tttcgcttat atgcctttaa aggcatggcg
     8521 cttaaataga taaaaacacc acaaaagcat aaaaaaccac acagtaaaac cgcaacataa
     8581 aacaataaca gataattaaa ccaaaaacag atagcgcatt gtgataatca ttcaatacta
     8641 aacaaaatat aaacagtgga gcaatatgtg attgactcat taagttagat ataaaaaata
     8701 catattcaat cattaaaacg attgaatgga gaacttttat gcgggcgaaa cttctgggaa
     8761 tagttctgac aacccctatt gcgatcagct cttttgcttc taccgagact atatcgttta
     8821 ctcctgacaa cataaatgcg gacattagtc ttggaactct gagcggaaaa acaaaagagc
     8881 gcgtttatct agctgaagaa ggaggccgaa aagtcagtca actcgactgg aaattcaata
     8941 acgctgcaat tattaaaggt gcaattaatt gggatttgat gccccagata tctattgggg
     9001 ctgcgggctg gactactctc ggcagccgag gtggcaatat ggtcgatcag gactggatgg
     9061 attccagtaa ccccggaacc tggacagatg aaagtaggca ccctgataca caactcaatt
     9121 atgccaacga atttgatctg aatatcaaag gctggctcct caacgaaccc aattaccgcc
     9181 tgggactcat ggccggatat caggaaagcc gttatagctt tacagccaga ggtggttcct
     9241 atatctacag ttctgaggag ggattcagag atgatatcgg ctccttcccg aatggagaaa
     9301 gagcaatcgg ctacaaacaa cgttttaaaa tgccctacat tggcttgact ggaagttatc
     9361 gttatgaaga ttttgagcta ggtggcacat ttaaatacag cggctgggtg gaagcatctg
     9421 ataacgatga gcactatgac ccaggaaaaa gaatcactta tcgcagtaaa gtcaaagacc
     9481 aaaattacta ttctgttgca gtcaatgcag gttattacgt aacacctaac gcaaaagttt
     9541 atgttgaagg cgcatggaat cgggttacga ataaaaaagg taatacttca ctttatgatc
     9601 acaatgataa cacttcagac tacagcaaaa atggtgcagg catagaaaac tataacttca
     9661 tcactactgc tggtcttaag tacacctttt aacgaagtta accagatttt ctccccggtg
     9721 gtattgtata accccgggga atttgtttac agttacaagt aggcaatatc cactaatttt
     9781 gtaagcattt aacctatgtc ataacgtgta atctcttacc gcaacacaag acctcaatat
     9841 attcaaatct tatctttatt ccgatattca gagacacaat gcgaaaaata attactcatt
     9901 tcaaagttgt tttaacgtta cttctaccag taaccgtatc tgcccagcag atacagtggc
     9961 aatcctgtat ggccagtcaa ttcaaccact ggtttggtga ggaaaaaccg tctcctgact
    10021 tactatgtgg ttatttgtct gttccattaa aatatacaga cacaggcgga gatgcttctt
    10081 atgaaaaaaa atcacaagtc aaactagcgt tgacaaaatt gccggcaaaa agcaagcata
    10141 aaggaagtat cctgataata agtggtggtc ccgggttacc aggcataaat ccttatatta
    10201 actttgactg gccagtcaca aatcttcgtg agtcatggga tattattgga tttgatcctc
    10261 gaggcgtcgg acagtccact ccgacaataa actgccggca atcagataca gagactcagg
    10321 aaaacataac cgaaaagcaa caagtattaa ataaaattaa tgcctgtatc cataataccg
    10381 gagccgaagt cattcgccat atcggctcta acgaggctgt atacgatatt gatcgtatta
    10441 ggcaagcctt gggggataaa caactgacag ccgtggcgta ttcgtatgga actcaaattg
    10501 cagccttata tgcagaacgt tttccctaca acgtaagatc tatcgttctt gatggagtcg
    10561 tcgatatcga tgacctggag gacaacttca catggcaact caaacaggca cagagttatc
    10621 aggaaacgtt tgatcgcttt gcatcctggt gtgcgcgtac aaaaagttgc ccgctttctt
    10681 cagacagaga taaggcaata actcagttcc atgagctatt atcaaaatta catcacaaac
    10741 ctttattaga cagtaagaga gaaaatatat cttcagatga actcatatca ttaacaacag
    10801 accttctgct atggcgttca tcatggccaa cccttgcaac tgccatacgc cagttctctc
    10861 aggggattgt cagtaatgaa attgaaactg cgctcagtgc tccgatagcc tcagaagagt
    10921 caagcgatgc ttcgggggta atcctctgtg tagatcaggg ggatgagcaa ttaacaccag
    10981 aagagcgaaa atcccgaaaa gacgctcttg cgaatgcctt cccggctatt aactttgaca
    11041 atggacgttc cgattcacct gatttttgtg aattatggcc aatacatagc gacctgaaca
    11101 aaactcgcct gaaaaatact gttctgccct ctggtttact gtttatagca cacaaatacg
    11161 acccaacaac gccctggatt aatgcccgta agatggcaga gaaattttcc agcccgttac
    11221 taacaataaa tggtgatggg catacattag ctctcaccgg agttaattta tgtgtagata
    11281 aagcagttgt acatcacctg atcactccac aaaaaataga aaatatatac tgcccaggaa
    11341 attctgaagc agaaatacaa taatttcaaa atattccgct a
//
LOCUS       DAZFKM010000151        11233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFKM010000151 DAZFKM010000000
VERSION     DAZFKM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..138
                     /locus_tag="ACHKAY_004286"
     CDS             4..138
                     /locus_tag="ACHKAY_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5702859.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            117..221
                     /gene="speFL"
                     /locus_tag="ACHKAY_004287"
     CDS             117..221
                     /gene="speFL"
                     /locus_tag="ACHKAY_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO5702860.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            532..2730
                     /gene="speF"
                     /locus_tag="ACHKAY_004288"
     CDS             532..2730
                     /gene="speF"
                     /locus_tag="ACHKAY_004288"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO5702861.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2727..4046
                     /gene="potE"
                     /locus_tag="ACHKAY_004289"
     CDS             2727..4046
                     /gene="potE"
                     /locus_tag="ACHKAY_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO5702862.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4102..5742)
                     /gene="pgm"
                     /locus_tag="ACHKAY_004290"
     CDS             complement(4102..5742)
                     /gene="pgm"
                     /locus_tag="ACHKAY_004290"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO5702863.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5768..6313)
                     /gene="seqA"
                     /locus_tag="ACHKAY_004291"
     CDS             complement(5768..6313)
                     /gene="seqA"
                     /locus_tag="ACHKAY_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO5702864.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6498..7262
                     /gene="ybfF"
                     /locus_tag="ACHKAY_004292"
     CDS             6498..7262
                     /gene="ybfF"
                     /locus_tag="ACHKAY_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5702865.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7402..7695
                     /gene="ybfE"
                     /locus_tag="ACHKAY_004293"
     CDS             7402..7695
                     /gene="ybfE"
                     /locus_tag="ACHKAY_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO5702866.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7835..8365
                     /gene="fldA"
                     /locus_tag="ACHKAY_004294"
     CDS             7835..8365
                     /gene="fldA"
                     /locus_tag="ACHKAY_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO5702867.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8575..8661
                     /gene="uof"
                     /locus_tag="ACHKAY_004295"
     CDS             8575..8661
                     /gene="uof"
                     /locus_tag="ACHKAY_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO5702868.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8654..9100
                     /gene="fur"
                     /locus_tag="ACHKAY_004296"
     CDS             8654..9100
                     /gene="fur"
                     /locus_tag="ACHKAY_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO5702869.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9184..9510)
                     /gene="chiQ"
                     /locus_tag="ACHKAY_004297"
     CDS             complement(9184..9510)
                     /gene="chiQ"
                     /locus_tag="ACHKAY_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO5702870.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9560..10966)
                     /gene="chiP"
                     /locus_tag="ACHKAY_004298"
     CDS             complement(9560..10966)
                     /gene="chiP"
                     /locus_tag="ACHKAY_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO5702871.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2721 a         2763 c         2808 g         2941 t
ORIGIN      
        1 ttaatgattt attatttggg gtatgatcgt tttttgttga tcttcttcac agattatagc
       61 catttcatgg atagaataac tctaccttca actgacacag caagaggtaa aggtaaatgg
      121 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
      181 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
      241 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
      301 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
      361 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
      421 aactactgat ttatttatca gcggtggagc tttgcttttt ttccggcgtg atcgatttct
      481 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaacaa aatgtcagaa
      541 ttaaaaattg ccgttagtcg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
      601 aatatagacg aaagtaatta tattgacgtt gccgccatta ttttatcagt cagtgatgtt
      661 gaacgtggaa aactcgatga aatagacgct actggctatg acattcctgt ttttattgca
      721 acggaaaatg aagaacgtgt acccgcagaa tatctgccac gtatttctgg tgtcttcgaa
      781 cattgcgaat cacgtaaaga gttttatgga cgtcagttag aaaccgctgc cagccattat
      841 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgttaa tcagggcaat
      901 agtgcttttg actgccctgg tcatcagggg ggtgagtttt tccgtcgcca tccggcaggc
      961 aatcagttcg tggaatactt tggcgaaatg ctcttccgct ctgacttgtg taatgctgac
     1021 gtagcaatgg gcgatctgct gattcatgaa ggtgcgccat gtattgcaca acagcatgcg
     1081 gctaaagtct ttaatgccga taaaacctat ttcgttttaa acggtacttc atcttctaat
     1141 aaagtggtct taaacgcatt gctgacgccg ggcgatctgg tgttgtttga ccgcaataac
     1201 cataaatcta accaccacgg tgcgttgcta caggctggcg cgacacctgt ttatctggaa
     1261 accgcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
     1321 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
     1381 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
     1441 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
     1501 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
     1561 gatccgggta ttctggttac gcaatccgta cataaacagc aggctggttt ttctcaaact
     1621 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
     1681 cgcatgaaca acgcctttat gatgcatgcc tccaccagcc cgttctatcc gctgtttgcc
     1741 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
     1801 tgtgtggtaa atggcattaa tgcccgcaag ctgatcctcg ataactgtca gcatattcgt
     1861 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
     1921 gcggttgatc tgcgcttctt ccagtttgta ccaggtgagc actggcactc ttttgaaggc
     1981 tatgcagaga atcaatactt tgtcgatcca tgcaaactgt tgctgacaac cccaggtatt
     2041 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
     2101 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta actccatcct cttcctgctg
     2161 actccggcag aagatatggc caagcttcag caacttgttg ccctgctggt acgcttcgaa
     2221 aaactgcttg agtccgacgc gccattagca gaagtgctgc cttccatcta caaacagcat
     2281 gaagagcgct acgccggtta taccctgcgt cagttgtgtc aagaaatgca tgatttgtat
     2341 gcccgtcaca acgtgaaaca actgcaaaaa gagatgttcc gtaaggaaca cttcccacgc
     2401 gtaagcatga atccgcaaga agccaactac gcctatttac gcggtgaagt ggagctggtt
     2461 cgtctgccgg atgcagaagg ccgtatcgct gccgaaggtg cgcttcctta tcctccgggt
     2521 gtgctgtgtg ttgttccggg tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct
     2581 ctggaagaag ggatcaacct gctgccaggt tttgcaccgg agctgcaggg tgtctatatc
     2641 gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa
     2701 agcaccctgt tgaaagggga aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg
     2761 ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga
     2821 caaagcttgc cgaagttggg acaatttcaa ttatctcctg gctggtgaca gccgtaggct
     2881 caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggcggta
     2941 tgggcggtta tgcggagtat gctttcggta aatccggtaa ctttatggcg aactatacct
     3001 acggcgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca
     3061 ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc
     3121 tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca
     3181 ttaccgtgtg gggggtcatt attccggtgg taggcctgtg cattatcggc tggttctggt
     3241 ttagcccgac gctttacgtt gattcctgga atccgcatca tgcaccgttc ttcagtgcag
     3301 taggttcttc catcgctatg acgctgtggg cttttctcgg tctggagtct gcgtgtgcga
     3361 atactgatgt agtggaaaac ccggaacgta atgtgccaat cgcggtactc ggcggtacgt
     3421 taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgcagggatt gtgcctaata
     3481 tggagctggc aaattcaacg gcaccatttg gcctggcctt cgcacagatg ttcacaccag
     3541 aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct
     3601 ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa
     3661 ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta
     3721 tccagagcgg gttggcgctg atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc
     3781 tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag
     3841 tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca aactttgttg
     3901 cttttgttgg cgcgatgtat agcttttatg cgctgtactc atccggggaa gaagccatgc
     3961 tgtacggttc catcgtgacc ttccttggtt ggacactgta tggtctggta tcaccacgct
     4021 ttgaactgaa aaataaacac ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg
     4081 cccttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc
     4141 ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt agatcttata
     4201 tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca tcactttcag
     4261 accgccgata gaagcaccat tacccggtgc cgccgtcaga cgtgcggtga tcgggtcacc
     4321 tgccagggtg ctggcgctca ccatttccgg agacagctta gacaacgccg ctttttgtgc
     4381 ggaagtcgca gcggcctgca aacggttgta gctcggcgca ccaaagcgtt ttgccagttc
     4441 gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca
     4501 cataatgatg ccgtctttat cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc
     4561 cccggcgctc tcttcgccgc caaagccgaa actgccgtcg aacagaccat caacaaacca
     4621 tttgaatcct accgggactt ctaccagttt acggcccaag gcgttgacca cacggtcgat
     4681 catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg
     4741 ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg
     4801 agtgacgata ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc
     4861 acgcagtgcc agcaggcccg ccatcgcaca ctcggaggag cagtccatac ggatcgtgcc
     4921 gtctttatca aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag
     4981 gttgaggttg taatactcgc caatacgctt ccagtattcg ataccggaac cgcccagcgg
     5041 atcaacgcca agcgtcaggc ccgctttctg aatcgccgcc atatcaacga tatcggccag
     5101 accttccaca aacggctgca ccagatcctg ctctttcaca tgaccggatg ccatcgcttc
     5161 gtcgagagag atacgcttca cgcctttcag gccatcggcc atcagtgcgt tggccctgtc
     5221 ttccaccact ttggtgacgt tggtatcagc cgggccacca tttggcgggt tgtacttgat
     5281 accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc
     5341 acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag tgaatccatt
     5401 gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta cggaaatgaa
     5461 cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag tgatgccatt
     5521 tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct
     5581 gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt
     5641 ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc
     5701 actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg
     5761 caacgtttta gatagttccg cgaaccttct caatcaattc cgccgggaat tgcatcgact
     5821 gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat
     5881 acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt
     5941 catctgccgc aaagtaaacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg
     6001 cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct
     6061 tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa
     6121 tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca
     6181 ccggagcagc aggctgtgat gaggcggaaa atttcaacat acgccgtaaa atgtcggatg
     6241 cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt
     6301 caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaggagtat
     6361 atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa
     6421 ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg
     6481 aaaaaaagaa ctttgccatg aaattgaata tccgcgcgca aactgcacaa aaccagcaca
     6541 ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac cttggcgtac
     6601 tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc
     6661 tttcaccgag agatccggta atgaattacc cggcgatggc gcaggatctt gttgatactc
     6721 tggatgcaca gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg
     6781 taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg atcgatatcg
     6841 caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcggctatc aacgcggtca
     6901 gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag catcttaatg
     6961 aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg
     7021 tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg
     7081 atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg
     7141 atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc gcgggtcact
     7201 gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt
     7261 aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga
     7321 ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg gctgattgtt
     7381 tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg
     7441 ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgaacag
     7501 ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc
     7561 gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga gtcgcgcaat
     7621 atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt
     7681 cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct
     7741 gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc
     7801 ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg
     7861 cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga
     7921 cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag cttatgacat
     7981 tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt
     8041 cttcccgact ctcgaagaga ttgatttcaa cggcaaactg gttgcgctgt ttggttgtgg
     8101 tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc gcgacatcat
     8161 tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc atttcgaagc
     8221 atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg aagaccgtca
     8281 gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg aagagttgca
     8341 tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt
     8401 aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctgt
     8461 acaatgtccc ggtgttcaag tggccttgcc gttgtaaatg taagctgtgc cacgttttta
     8521 ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata
     8581 cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca
     8641 ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac
     8701 gcttccacgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc
     8761 ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata
     8821 tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg
     8881 taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt gcctcgactg
     8941 cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc
     9001 aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg
     9061 cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag
     9121 ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag ttacagctct
     9181 cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg ctttcaccgc
     9241 ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta cgcgtacact
     9301 ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta acacgctctg
     9361 gcaggcttca attttttctg gcgaaccttg tgcggtgttg atacaggcgc tgtatgcctc
     9421 tttcagacga ctgtcttcct gtggcgcggt tgattgcgca caagctacca gccccgatgc
     9481 cattatggcg atgagaatca gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca
     9541 aaacctgccg cgtcgggcat cagaagatgg tgaatggtgc gattaccata aatttcacgt
     9601 cacgctcatc ctggaagatg ttaccgtaac cgccgcccca gcttgggatg tcagagtggt
     9661 tgtcgtattc ggtgaagtgc agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg
     9721 tgtagaccgc atccaggctg taggcagact cttcaatagt ccggtttttg tcgtagtacg
     9781 catccggatt gctctgccag gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc
     9841 cgatggcgaa gcctggaaga ttccagtttt tcaggtcata catcgcaccg aagaagaccg
     9901 ctttttcgcc gttggcgttg aagtcggaac ggttatccca ccagatatcc aggcggccgt
     9961 tggaggaagc gtaggttgga gtcatacgtt gcaggaagta tccctgctga ccgtcagcct
    10021 taacccaggt gccttcgagg cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca
    10081 acgcttgcag ccaggcggtg ccgtcataaa ggtcgttgac gctgcgatcg tcaactttat
    10141 cgcgcgtacc atagaactgg tagctggtgg ttaacgggct accggcgata tcaaatttgt
    10201 agctggcttt ggcaaaatat tgatcgatat acccttccgc ctgaccaaat gccgcttcca
    10261 gtacgaagtt atttttgaag tcgtatttcg caccgatgga gtgcaggtaa tcaactttgg
    10321 tggttttatc gttctgataa aactcatcca tttccagatg ccacggcgct ttgtattcgt
    10381 tggtccacat gtaggagaaa ctcaaggcac cagcatcgcc gtaatcaaaa ttcgccccgg
    10441 cttccgcccc ctgataagta cctggcataa agctccagtg cggcgctaac agcgtttgac
    10501 cagttggctg aatgtaacct gccctcgccc aaaccggacc gtatttaaat ttggccgcag
    10561 ctttatacag gcttataccg cttttgtcgc cggaccagtc ttcgtcatag gctttattac
    10621 tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg
    10681 ccgtaaacgc agcaatatca aggccgaaca tatcagcagc ataaccggac tgaaaatcga
    10741 ggttggcatt ccaggtggag tgagaaaggt tggttttgta tttgtcgccg tcggtaacat
    10801 ctttacggtc gcgttcacgc tgccagtaat atataccgcc ggttaaggtt gaatcgtcga
    10861 tgaatccttc ggcgcgagcc tccggcattg ccataaagcc cgacattgct gtaacaccgg
    10921 cgatagccag cgccagcgta ctacgtttgc cactaaacgt acgcatgggt taatcctctt
    10981 tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa ataaaaatta aaaaagtagc
    11041 gttgagaaaa gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat
    11101 ctctttctgt aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt
    11161 tacaaggctt aagtttgcgg ttgaaaggct tgcagaagaa taaaagcgtg aatttccagc
    11221 aagatgaaag cgg
//
LOCUS       DAZFKM010000152        11217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFKM010000152 DAZFKM010000000
VERSION     DAZFKM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(47..400)
                     /locus_tag="ACHKAY_004299"
     CDS             complement(47..400)
                     /locus_tag="ACHKAY_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO5702872.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHKAY_004300"
     CDS             complement(538..2184)
                     /gene="groL"
                     /locus_tag="ACHKAY_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO5702873.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2228..2521)
                     /locus_tag="ACHKAY_004301"
     CDS             complement(2228..2521)
                     /locus_tag="ACHKAY_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO5702874.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHKAY_004302"
     CDS             2797..4053
                     /gene="yjeH"
                     /locus_tag="ACHKAY_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO5702875.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(4069..4545)
                     /locus_tag="ACHKAY_004303"
     CDS             complement(4069..4545)
                     /locus_tag="ACHKAY_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO5702876.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            4882..6318
                     /gene="aspA"
                     /locus_tag="ACHKAY_004304"
     CDS             4882..6318
                     /gene="aspA"
                     /locus_tag="ACHKAY_004304"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO5702877.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHKAY_004305"
     CDS             6436..7737
                     /gene="dcuA"
                     /locus_tag="ACHKAY_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO5702878.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7853..8191
                     /gene="cutA"
                     /locus_tag="ACHKAY_004306"
     CDS             7853..8191
                     /gene="cutA"
                     /locus_tag="ACHKAY_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO5702879.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHKAY_004307"
     CDS             8167..9864
                     /gene="dsbD"
                     /locus_tag="ACHKAY_004307"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO5702880.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9901..10476
                     /locus_tag="ACHKAY_004308"
     CDS             9901..10476
                     /locus_tag="ACHKAY_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5702881.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            10583..10658
                     /locus_tag="ACHKAY_004309"
     tRNA            10583..10658
                     /locus_tag="ACHKAY_004309"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10616..10618,aa:Phe,seq:gaa)
     gene            10856..>11217
                     /locus_tag="ACHKAY_004310"
     CDS             10856..>11217
                     /locus_tag="ACHKAY_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO5702882.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT         2637 a         2943 c         2786 g         2851 t
ORIGIN      
        1 agcgcatcag gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact
       61 tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
      121 acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
      181 cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc
      241 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
      301 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
      361 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
      421 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
      481 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
      541 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
      601 atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga
      661 agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
      721 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
      781 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
      841 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
      901 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
      961 ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat
     1021 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
     1081 gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
     1141 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
     1201 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
     1261 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
     1321 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
     1381 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat
     1441 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
     1501 agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
     1561 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
     1621 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
     1681 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
     1741 accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc
     1801 aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
     1861 agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt
     1921 accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat
     1981 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
     2041 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
     2101 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
     2161 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
     2221 gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
     2281 cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
     2341 ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
     2401 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
     2461 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
     2521 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
     2581 tttcccggct ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag
     2641 aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
     2701 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
     2761 gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg
     2821 gggctggccc aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc
     2881 gttcctgcgt tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg
     2941 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
     3001 gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc
     3061 ggctggttgt ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc
     3121 gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
     3181 acgctggcgc tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa
     3241 accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc
     3301 aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct
     3361 gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg
     3421 gaatttaaaa atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca
     3481 ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa
     3541 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
     3601 tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag
     3661 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc
     3721 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg
     3781 agcacgttgg tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat
     3841 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga
     3901 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc
     3961 ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa
     4021 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
     4081 atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa
     4141 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa
     4201 atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc
     4261 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac
     4321 acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac
     4381 gaaattctta aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac
     4441 cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
     4501 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
     4561 tagcagccgg taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
     4621 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
     4681 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
     4741 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
     4801 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
     4861 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
     4921 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
     4981 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
     5041 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
     5101 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
     5161 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
     5221 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
     5281 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
     5341 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
     5401 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
     5461 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
     5521 gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
     5581 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
     5641 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
     5701 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
     5761 atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac
     5821 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
     5881 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
     5941 gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc
     6001 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
     6061 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
     6121 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
     6181 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
     6241 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
     6301 gatgaaagcg aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg
     6361 tgcctttttt cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa
     6421 acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg
     6481 ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt
     6541 gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg
     6601 gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag
     6661 acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc
     6721 tatttcctga ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc
     6781 gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta
     6841 tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc
     6901 gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc
     6961 ctgctggcgg ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct
     7021 gacgatccga tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag
     7081 cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt
     7141 ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg
     7201 atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt
     7261 atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc
     7321 gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc
     7381 gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc
     7441 atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg
     7501 ccgatggccc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg
     7561 tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac
     7621 acgggtacaa cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact
     7681 ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga
     7741 ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc
     7801 cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga
     7861 tgaagaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac
     7921 agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat
     7981 ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca
     8041 gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca
     8101 tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta
     8161 cctctcatgg ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt
     8221 gccggattat tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct
     8281 tttgattttc agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac
     8341 tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag
     8401 ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat
     8461 cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc
     8521 taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta
     8581 agcgaagtgg tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag
     8641 cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc
     8701 tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa
     8761 cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca
     8821 ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg
     8881 ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca
     8941 atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg
     9001 agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga
     9061 ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa
     9121 agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg
     9181 ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg
     9241 gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag
     9301 cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt
     9361 ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata
     9421 atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg
     9481 acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag
     9541 ttaaatcagg cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac
     9601 tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa
     9661 gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg
     9721 gcactgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa
     9781 ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca
     9841 catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc
     9901 gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc
     9961 aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc
    10021 ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata
    10081 gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg
    10141 gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt
    10201 atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag
    10261 caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat
    10321 ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg
    10381 gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc
    10441 ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt
    10501 tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga
    10561 ttacggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa
    10621 tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca
    10681 cggaggtccg tttttcgttt cagaacacca tgatttaagt gttcttcctc caaatcaatt
    10741 ctaccgaact caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc
    10801 ataccccagt tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc
    10861 actgactgac gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga
    10921 cggggctggc atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta
    10981 tcgtattctg ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc
    11041 cgaagctcgt acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg
    11101 cgaacagaaa agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc
    11161 acgacgctgg catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZFKM010000153        11109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFKM010000153 DAZFKM010000000
VERSION     DAZFKM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..727)
                     /locus_tag="ACHKAY_004311"
     CDS             complement(137..727)
                     /locus_tag="ACHKAY_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5702883.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(1044..1277)
                     /gene="ydfK"
                     /locus_tag="ACHKAY_004312"
     CDS             complement(1044..1277)
                     /gene="ydfK"
                     /locus_tag="ACHKAY_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO5702884.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            complement(1346..1459)
                     /locus_tag="ACHKAY_004313"
     CDS             complement(1346..1459)
                     /locus_tag="ACHKAY_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO5702885.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            2062..3345
                     /gene="ydfJ"
                     /locus_tag="ACHKAY_004314"
                     /pseudo
     CDS             2062..3345
                     /gene="ydfJ"
                     /locus_tag="ACHKAY_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            3434..4894
                     /locus_tag="ACHKAY_004315"
     CDS             3434..4894
                     /locus_tag="ACHKAY_004315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO5702886.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(4930..5133)
                     /gene="ydfZ"
                     /locus_tag="ACHKAY_004316"
     CDS             complement(4930..5133)
                     /gene="ydfZ"
                     /locus_tag="ACHKAY_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO5702887.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(5311..5997)
                     /gene="rspR"
                     /locus_tag="ACHKAY_004317"
                     /gene_synonym="ydfH"
     CDS             complement(5311..5997)
                     /gene="rspR"
                     /locus_tag="ACHKAY_004317"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO5702888.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(6086..6832)
                     /gene="ydfG"
                     /locus_tag="ACHKAY_004318"
     CDS             complement(6086..6832)
                     /gene="ydfG"
                     /locus_tag="ACHKAY_004318"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO5702889.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            6969..9014
                     /gene="dcp"
                     /locus_tag="ACHKAY_004319"
     CDS             6969..9014
                     /gene="dcp"
                     /locus_tag="ACHKAY_004319"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO5702890.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(9059..9577)
                     /locus_tag="ACHKAY_004320"
     CDS             complement(9059..9577)
                     /locus_tag="ACHKAY_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO5702891.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            9853..10245
                     /gene="ydeI"
                     /locus_tag="ACHKAY_004321"
     CDS             9853..10245
                     /gene="ydeI"
                     /locus_tag="ACHKAY_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO5702892.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            10500..>11109
                     /gene="dgcZ"
                     /locus_tag="ACHKAY_004322"
     CDS             10500..>11109
                     /gene="dgcZ"
                     /locus_tag="ACHKAY_004322"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO5702893.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT         2877 a         2404 c         2704 g         3124 t
ORIGIN      
        1 tcaactgcac aggatattga atggccagca ctgccgtagg gtaaaacata taaattctat
       61 aattagatgt atctttccat ttacggcaag gaagggggct tggaagacgt aaagcatctc
      121 acaccgagat tattttttat atgtcaggtg tctgaagttt tgctttggct cttaaaatgg
      181 tttgccgcga ggttttgaat tcccgggcaa tggcacttat acttacacct gacttaattc
      241 gttcgaatac cacctgtttc tgttcttcat ttaacacagg tggtcgacca aaacgtttcc
      301 ctgcgccgcg ggctcttact atcccggaat gagtgcgttc aagtaaaagg tctcgttcaa
      361 attcagcgac tgctgaaatt acgtgcatca tcatttttcc tgttggactg gtcaggtcaa
      421 tgccccccaa tgctaagcaa tgcactctga tacctgtttc ggtcagttgt tccactgttt
      481 tcctgatatc cattgcatta caaccaaggc gatccagttt tgtcacaatc aattgatcac
      541 cacatttcag gcgagcaagc aaccggttaa aaccaggacg ctcactggtt gctgctgagc
      601 cgctaatgtg ttcttcgatt atttgctgag gtttgatttt aaaacctgca ctttcgattt
      661 cccggcgttg attttcgatg gtctgatcca gcgttgatat ccgacagtaa gcaaaaattc
      721 gagacatagt gagactctat acgaaattgg tgttcatatc ataatgcatc tcagaaaata
      781 attttgatta tttttgtgca tatttgtatg tacacgttcg aaaataaacg aatgcgtatg
      841 caaccccgta attttggtga gacccaaaat cgattttgtg aaaaatggct ttaactcggt
      901 ttgtttttcg agttccgggc ggactcaagg aagaagaata gtgttgcgtg ttattttaac
      961 cagatttcaa gttgtttggt cgtggaaaag tggagcaaaa tgttgttaaa gtggaaaaat
     1021 gataaaaaag taagtttatt atattacatt ttaccattta aattttggtt gtctttaaga
     1081 actgatatcg ctgtttgtaa taattctttg ttatccagcc atgatttttt ctttatgttt
     1141 ccttcaatgt aatcaagcaa tgttctggta ttgataggtc ttccctgttt tgctacttcc
     1201 actacagcat cccctaggat aattcttact tcaggaagct gcgcagggaa ccactttagg
     1261 gtgtcttttg atttcatgaa gatattcctt aaaatattat tgattttcat tgcgatattg
     1321 tatgtctgat tcaggatatg ttgacttata catcggtttt gtctgggtta ttggatatgc
     1381 caatccctaa ttttattagg gcatgactaa aaatgctgaa tatgataagg agagaagtga
     1441 ttatcagtat gctgttcata tagcctcgaa ttagtaatgt gttatatatg atatagttga
     1501 caatttttat cttgggtgtt cttaaagttc gtagataaac attgtcgttt caggtataca
     1561 ggaatgctaa caggtggcag caaaaatcag gcggtttatg gcgcaagctg aagcggcaac
     1621 tgcaaactat cttatgcaga gactctacac ggattgggtt taaaagtata catagataac
     1681 agtttttatc tgaaaaagaa aaatatcaag gtgatatagc ctatatgcct ttgatgtgga
     1741 ggaatgaatg tgatgggagt gatgtatctg aatagttgaa aaaccgcaga cacaccttat
     1801 gcaagaacgt gctgcggttg gctggtaaat ttttcgatag tgcgagtatt gaatgatttc
     1861 cagccgttat tgattttacg cgtaaatcca tgaaaaaact acttatctgt tggggagttt
     1921 tttggggcat atatgggaca gaaataggcc ccagatagac actgagatcg aacttaggat
     1981 gcttttaaaa aaatgcaact atctgaaaaa acctagaaaa cgccaaggaa accacaggat
     2041 gggaaaaaac acctgtgaat tatggatttc cagttatatt cgctcggcgc agcgttagtg
     2101 tttcatgaaa tatttttttc tgaatcatca acggcaatgg cgttaatttt ggcaatggga
     2161 acctacggtg caggttatgt ggcgcgtatt gtcggagcat ttattttcgg caaaatgggc
     2221 gacagaatag ggcgtaaaaa agtgctcttt attaccatca ccatgatggg gatctgtacc
     2281 accttaattg gtgtgttacc gacctatgca cagattggtg tttttgcccc catcttgctg
     2341 gtgacgctgc gtattattca ggggttgggt gcaggtgcgg aaatttccgg tgccggtacg
     2401 atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt tgtagctatg
     2461 ggaactaact gcggaacctt gagcgcaacg gcaatctggg cctttatgtt cttcattctc
     2521 agtaaagagg aactgcttgc gtggggatgg cgtataccgt tcctggcgag cgttgtcgtg
     2581 atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt tgagaaggtt
     2641 aacgacagca accaacccac agcaaaacct gcacctgctg gtagcatgtt ccagagcaaa
     2701 tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg gttaattcag
     2761 actttccttg caggctattt agtgcagacg ttattgttta acaaagcaat tccaacagat
     2821 gcattgatga ttagttcgat tctcggcttt atgaccattc cgttccttgg ttggttatcc
     2881 gataaaattg gtcgccggat cccgtatatt attatgaata cctccgcgat tgtgctggca
     2941 tggccaatgc tttctatcat tgtagataaa agctatgccc cgagcaccat tatggttgca
     3001 ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa cattaccatg
     3061 gcagaaatgt tcggctgtaa aaaccgcttt acccggatgg ctatttctaa agaaattggt
     3121 ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac catgacggaa
     3181 tcctggtatc cgatcgccat tatgatcatg gcatattcag taattggttt aatctctgcg
     3241 ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc tgcggaagat
     3301 caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaatcct caatcccttc
     3361 tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc atatttatat
     3421 ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc ctgtttatga
     3481 tcgtaataac ctggccccaa gaattattca tttaggcttt ggtgcatttc accgtgcgca
     3541 tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg gatattatga
     3601 ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag ataatcttta
     3661 taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg tcgttaaaaa
     3721 agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt gtgaaccgca
     3781 aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc cggcgatcgg
     3841 acagttaatg ctcgatcacc cgatggtcgc tgccgacgtg caaaatcccc accagccgaa
     3901 aacagcaaca ggagtgattg ttgaggcgct ggcccgccgt aaagcggcag gacttcccgc
     3961 atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt
     4021 cacttcatac gcgaaagctg ttgatgtaaa actggcacaa tggatcgaag ataacgtgac
     4081 tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata cgctggcgaa
     4141 aatcgaacaa cttaccggtg tgggcgatcc tgcgggcgtt gcctgtgaac ctttccgcca
     4201 gtgggtaata gaagataact ttgttgccgg acgtccagaa tgggaaaaag cgggagccga
     4261 actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca acggcagtca
     4321 ttcattcctg gcgtatttgg gttatcttgc tggatatcag cacattaatg actgcatgga
     4381 agatgaacat tatcgttatg cggcgtatgg cttgatgttg caggaacaag cgccgacgct
     4441 gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac gctatagcaa
     4501 cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggtagccaga aattgccaca
     4561 gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg atctgctggc
     4621 gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg gaaatctgat
     4681 agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta gtgccgaagg
     4741 gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg atttgccaga
     4801 caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag cgcatggcgc
     4861 gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc atcggcggca
     4921 ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt tctcttcaca
     4981 accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac cttcagtatc
     5041 aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc tgccagtggt
     5101 gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc tgatttacag
     5161 tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata ttttttgttt
     5221 ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg ggaaaagcct
     5281 ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt cgctgttttc
     5341 ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca ttgcacgttc
     5401 taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct ggcgtaacag
     5461 catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa cggtcgcttt
     5521 caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca ggagttgatg
     5581 gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg caatgcgttg
     5641 ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc tcgccgcccg
     5701 acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgtttcgca cctgggccat
     5761 ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc cgttttccgc
     5821 cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa cagaaacttc
     5881 tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc gacgaagaat
     5941 acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga cggtcattcg
     6001 ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac aagcaataac
     6061 ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg gcatagcttt
     6121 gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc gttgacaccc
     6181 accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga taggtttttt
     6241 ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca cccaccagac
     6301 ccggttcgat gtcggtgacg cgcaccgccg taccatgcag gtccgtacgc agattcaggc
     6361 taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca taaggccagc
     6421 tacctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc atacccggta
     6481 agacggcgcg cgtcatatat accaggcctt tgttgttggt atcaatcatc gtttcccagt
     6541 cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca ttatttacca
     6601 ggatatcaat attgcaccac tcggcaggga gcgatgccag catctcttca atagcggcgc
     6661 ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct tttaactcct
     6721 gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga ataaaacgac
     6781 gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc atctcactgt
     6841 tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg ggtaatactg
     6901 gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct cgcgttcagg
     6961 agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg tatctggctc
     7021 ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag ggaatgcagc
     7081 aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat ttcaacaata
     7141 ctattctggc actggaacaa agcggagaat tacttacccg cgttaccagc gtcttttttg
     7201 cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag ttttccgctg
     7261 aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg cgggtagatg
     7321 ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc ctggtggagg
     7381 tgattcatca acgttttgtc cttgccggag ccaaacttgc gcaagctgat aaagcaaaat
     7441 taaaagtact gaatacagaa gctgcgacac tgaccagtca gtttaaccag cggttactgg
     7501 cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca ggaatgagtg
     7561 agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat aacaaatggc
     7621 tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc gatcgtgcga
     7681 cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat ggcaatgata
     7741 cccgcgctat cattcaacgt ctggtggaga tccgtgcaca acaggcgaca ttacttggtt
     7801 ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacacct gaagcagcgc
     7861 tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat gaattagcct
     7921 ccatacaggc ggttattgat aagcagcagg gcgggtttag cgcgcagccg tgggactggg
     7981 cattttatgc cgaacaggta cggcgggaga aatttgatct tgatgaggcg cagctcaagc
     8041 catattttga attaaacacg gtgttgaatg aaggtgtatt ctggaccgcg aatcagctct
     8101 tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta ccatcctgac gttcgtgtgt
     8161 gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat ttcttcgccc
     8221 gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca acgcttaatg
     8281 aaacgcattc ggtaatttat aacgtttgca attatcagaa acccgctgcc ggtgagcctg
     8341 cgttgttact ctgggatgat gtcataacct tattccatga atttggtcat acgctgcacg
     8401 gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg cgtgattttg
     8461 tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta ttcgctcgct
     8521 acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag aaaatgcgta
     8581 atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc gcacttctcg
     8641 atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat gattttgaat
     8701 tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc tatcgcagca
     8761 gttatttcgc ccatattttt ggtggcggat atgccgcagg ttattacgct tatctgtgga
     8821 cacaaatgtt ggccgatgat ggttatcagt ggtttgttga gcagggcgga ttaacgcgtg
     8881 aaaatgggct gcgttttcgc gaggcgatcc tttccagagg taacagcgaa gatctggaac
     8941 gcctgtatcg acaatggcgc ggtaaggctc ctcagattat gccgatgctg caacatcgtg
     9001 gcttgaacat ataaacacca ccaatggctg tcctggagat atcgccaggc cagcctggtt
     9061 atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct aataccgttt
     9121 cgcagtcgcc agcaaaatgc ataaccgcag tataggtctg gcctttaata tgccacgcga
     9181 accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg tagccggtaa
     9241 tggcaatact tacatcagca tccgcacgct ctatggctcc ggttgccatt tctgctgcca
     9301 ctttctcact caccgcagaa tatcgttcaa gagattgctg gcttacgctg aggattttca
     9361 tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt gtatcttcag
     9421 ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta gttaagcgta
     9481 attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata gtgtcggtat
     9541 ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca cgctattata
     9601 tgtaaaggtg atttgctttt tctgtatgct cgtcaaatat aataattttc aaacatataa
     9661 atccgattat tctgaaaata tgtttaagac tcttcaatct tcttaacttt agtgagttaa
     9721 attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac aattggcagt
     9781 attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac caaacgcaat
     9841 taaggagtta gtatgaaatt tcaggccatt gtattagcaa gttttcttgt catgccttat
     9901 gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc gcctcatgca
     9961 atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga tttcgcaaaa
    10021 aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca caaaggagag
    10081 gaccgttacg tttttcgcga taagagcggt gaaattaatg tcgttattcc tgcggccgtc
    10141 tttgatggac gagaagttca gccggaccag atgatcaaca ttagcggcag tctggataag
    10201 aaaagcgcgc cggccgtcgt tcgggtcact catttgcaga aataaaaata aattagcctg
    10261 atggcctgaa ctactgggcc attagggtat tgggttatgc tatttgtcat ccttttattt
    10321 acgataatgc agaggtgatt tacagaatta aacacttttt tatccgctga ttttgtttta
    10381 aatcacaatg ttatctcaga gatgactcat aacttcggat ttctatgaaa atattgacgt
    10441 aattttgctt ttctctcgtt agaatagcgc gcacaaggaa ctgtgaaaaa ggagtggcaa
    10501 tgatcaagaa aacaacggaa attgatgcca tcttgttaaa tctcaataag gctatcgatg
    10561 cccactacca gtggctggtg agtatgtttc acagcgtggt cgcgagagat gccagtaagc
    10621 cagaaataac ggataaccat tcttatggac tgtgccagtt tggtcggtgg attgatcatc
    10681 tggggccact cgataacgat gaattacctt acgttcggct aatggattct gcccatcaac
    10741 atatgcataa ctgtggtcgg gaattaatgc tggctattgt tgaaaatcac tggcaggacg
    10801 cgcatttcga ctcctttcag gaggggttgc tttcttttac tgcggcatta accgattaca
    10861 aaatttattt gctgacgatc cgtagcaata tggatgtttt gacgggattg ccggggcgtc
    10921 gggttcttga tgaatccttt gatcatcagt tacgcaacac tgagcctctg aatctttatt
    10981 taatgttgtt ggatattgac cgatttaaat tggttaatga tacctacggg catttaatcg
    11041 gcgatgtagt attacgcacc ctggcaactt acttagccag ttggacgcgt gattacgaaa
    11101 cggtttatc
//
LOCUS       DAZFKM010000154        11052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFKM010000154 DAZFKM010000000
VERSION     DAZFKM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..457)
                     /locus_tag="ACHKAY_004323"
     CDS             complement(<1..457)
                     /locus_tag="ACHKAY_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702894.1"
                     /translation="MNTGYSPEQGRGFVRPEKQNLQNFAEIIPVISGLTGGSETNIVN
                     ARALQMFDDKKGVNLTYTPDGNQNMSIISESGFYKLIKTKSAPLPERLCEQLTYCAKN
                     ESEQWDYINHVEKRHNCRITGKTKATRYGGPSTQATRYPQRMSIANNATF"
     gene            complement(510..698)
                     /locus_tag="ACHKAY_004324"
     CDS             complement(510..698)
                     /locus_tag="ACHKAY_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5702895.1"
                     /translation="MLSTDRFIREKECQQLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCEAVH"
     gene            complement(1073..2302)
                     /locus_tag="ACHKAY_004325"
     CDS             complement(1073..2302)
                     /locus_tag="ACHKAY_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5702896.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGARPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQK
                     ALKPVTVGDALTYWLESYVKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLKHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGTHENVTTLPIAREI"
     gene            2551..3672
                     /gene="roxA"
                     /locus_tag="ACHKAY_004326"
                     /gene_synonym="ycfD"
     CDS             2551..3672
                     /gene="roxA"
                     /locus_tag="ACHKAY_004326"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO5702897.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(3818..5047)
                     /gene="pepT"
                     /locus_tag="ACHKAY_004327"
     CDS             complement(3818..5047)
                     /gene="pepT"
                     /locus_tag="ACHKAY_004327"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO5702898.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            5297..6433
                     /gene="potA"
                     /locus_tag="ACHKAY_004328"
     CDS             5297..6433
                     /gene="potA"
                     /locus_tag="ACHKAY_004328"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO5702899.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            6417..7280
                     /gene="potB"
                     /locus_tag="ACHKAY_004329"
     CDS             6417..7280
                     /gene="potB"
                     /locus_tag="ACHKAY_004329"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO5702900.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(7513..7662)
                     /locus_tag="ACHKAY_004330"
                     /pseudo
     CDS             complement(7513..7662)
                     /locus_tag="ACHKAY_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            7786..7871
                     /locus_tag="ACHKAY_004331"
                     /pseudo
     CDS             7786..7871
                     /locus_tag="ACHKAY_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(7894..8484)
                     /gene="espM2"
                     /locus_tag="ACHKAY_004332"
     CDS             complement(7894..8484)
                     /gene="espM2"
                     /locus_tag="ACHKAY_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO5702901.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            8668..9318
                     /locus_tag="ACHKAY_004333"
     CDS             8668..9318
                     /locus_tag="ACHKAY_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5702902.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            9393..10451
                     /gene="espW"
                     /locus_tag="ACHKAY_004334"
     CDS             9393..10451
                     /gene="espW"
                     /locus_tag="ACHKAY_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO5702903.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNARNQNKFPIDVKETVIKLQGQKFTCIEDFHKKYNETRLKANTNIQQKQI
                     ADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISPDDTDKITGRKFS
                     ELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYSDEVLQRNGTLIPTP
                     LHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILACSP
                     RNYGLHHNIKTWDPLVRN"
     gene            complement(10579..>11052)
                     /locus_tag="ACHKAY_004335"
     CDS             complement(10579..>11052)
                     /locus_tag="ACHKAY_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5702904.1"
                     /translation="EPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQ
                     SVMAEHRENKTSNKILMEKINSCLFRPDSNHFSCPESFLTCPITLDTPETGVFMRNSR
                     GAEICSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDSKKEAFVASDA"
BASE COUNT         2944 a         2615 c         2416 g         3077 t
ORIGIN      
        1 caaatgtggc attgttggca atgctcatgc gttggggata acgtgtagct tgtgtcgagg
       61 ggccaccgta gcgggtggcc tttgttttgc ccgttattcg gcaattgtgg cgcttctcca
      121 catggttgat ataatcccac tgctcagatt cattttttgc gcagtaggtt aattgttcac
      181 aaaggcgctc cggcaacggg gcgctttttg tttttattag tttgtagaaa cctgactcag
      241 agataatgct catattctga ttgccgtcag gggtgtaagt taaatttact ccctttttat
      301 catcaaacat ctgcaacgct ctggcgttaa caatattggt ttcactcccg ccagtaaggc
      361 cggaaataac cggaataatt tcggcaaaat tttgcagatt ctgtttttca ggacgaacga
      421 agccccgccc ttgttcggga gaatatccag tattcatggt tagatctctg tattagtgga
      481 tgggtggcgg ctgtgtgccg ccagcctgat tagtgaactg cctcgcagct gtccttccat
      541 gccagaactt cggataaaga ccagccaacg gaacgaccgc ccagcttacg ccgtgatggg
      601 aattgtccgg ctttttccag gcggtaccga catgagcggc tcaggcctgt aagttgctga
      661 cattcttttt cacgtataaa gcgatctgtg cttaacacaa ttcccccttt gttgtttctt
      721 aaagagtcat tgggtggatt attgggttgg gttgtgtcgg attgtgtcgg attgtgtcag
      781 ggtgtttgtg gaatggcaaa cactggcggc ggtcgtttta cagaagcagg gggaatgaga
      841 ataaaaactc ttttaattca ttatgatgca aaggcataaa attttacttt tacgcctttt
      901 ttacatattt ttaagagtga ttcgccagtg tataaaaaaa cagtacacct ataaaaatca
      961 aatggttatt acgatggctg atttttaacc cgttgttcca ttttgttcct tgttgttcgt
     1021 cgttgtgtct cgttgtttca cgttgtccgc atcctgaaaa atcgcaaaaa aattatattt
     1081 ctctggctat tggcaatgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct
     1141 ccgtccacat atccagcgcg tcacgtttcg catccagata acgggaatgg ttatagactc
     1201 gttgcatccc tggcatctga tgacctgtaa gctgctccac gacgtgagga tccacgccta
     1261 aatcgttcag catcgttgta aaggtgcgcc ggatgtcatg cagtgaccag tgagggtgtt
     1321 ttagcctcct gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc
     1381 ccagcaataa gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg
     1441 cttccggtat gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc
     1501 agagcatttc ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc
     1561 atccaaaaac aatcaggagg cggattagtg cgacgtagta aggggaaaat atttttttgt
     1621 ccagtgcctg caataattcg ccaagttctt tggtgcttaa gacacgctcg cttatatccg
     1681 gttttttccc aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc
     1741 gcctccggca gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag
     1801 gcgttcgctt tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca
     1861 gcggcatagc accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat
     1921 aatccacgcg gttttccttt acgtaagact caagccagta ggtaagcgca tcgccaaccg
     1981 ttacgggctt taacgctttc tgtacggtgt aattcatctc atgacgtgga tttttcccct
     2041 cagcaagcca agcgcgacac tgtgccgctt tttccctggc agctttcaga ctcagatcag
     2101 gataatttcc cagcttaatc cgttccggtc gtgctcccct tcccgttccg gccctgtagg
     2161 tgaaatacca ggttaaaagg ccgctggttg aatgcctgac gctcaggttt ccaccgtcat
     2221 taagaaaggc tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca
     2281 gtttgttaag tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta
     2341 actacaccga tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca
     2401 caaatctgtt tataatcaaa aacatagtga acaatctgaa acattatgaa acggcaaaaa
     2461 acactaattg ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg
     2521 gttgcagata ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc
     2581 gattttcttg aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat
     2641 tttattgacc cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac
     2701 agtcgactgg tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc
     2761 tacgatcatc tcggtgaaac caactggtca ttactggtac aggcagtgaa ccactggcat
     2821 gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat
     2881 ctgatgatct ctttttctgt accgggcggc ggcgtcggcc cgcatctcga tcagtacgac
     2941 gtgtttatca ttcagggtac cggtcgtcgt cgctggcgag tgggcgaaaa gctgcaaatg
     3001 aaacagcact gtccgcaccc ggatctgtta caggtcgatc cgttcgaagc catcatcgat
     3061 gaagagctgg agcctggcga tattctttat attccgccag gattcccgca tgaaggctac
     3121 gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta
     3181 attagcggat ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat
     3241 ccggatgttc cacctcgcgc tcatcctgcg gacgttctgc cgcaagagat ggataaactg
     3301 cgtgagatga tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag
     3361 tttatatccc agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca
     3421 gatgaaatct acgatgcgct gaaacaaggt gaagtgctgg tgcgcctggg tggtctgcgc
     3481 gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt
     3541 ccggcactgg atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg
     3601 ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc
     3661 ttcgaagggt aagtttggat ttaagccgga tgcggcgctg attgcgcctt atcgtgccta
     3721 catgcactga acgcctgtag gcacgataag tatgtggacc agtatcaata taacggtttc
     3781 atcccggtaa ttggcagtcc acatacgcat cgggcaatca cgacttccgt tgcgccgtta
     3841 actcggcaat acggacaatc acctgcaccg ctttttccat accttccaga gtcacaaact
     3901 catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcacggt aatcccataa
     3961 acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc
     4021 agtcacgcat cgcctgctgg gcgatatcga taatatgcgg atgctcaacc actttctcgc
     4081 gcatattgta gtaactgtct tcaatcacca gttcaatgta gcaatcagga tgtaaccctt
     4141 tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt
     4201 cgaaatcacg gatgatgtag tgcatatcgg ctcggtcaac ggtgcctttc atgctcgcca
     4261 ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg
     4321 catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccggat
     4381 gaacattgtt accgacaatt ttgatattga ccgatgcggc gttgaagttt tcaaactcca
     4441 gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat
     4501 caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac
     4561 catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg
     4621 ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcaacgtc tgacccagta
     4681 gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa
     4741 tatcgccacc gcgatagttt tcaacaattt gcggattcac atttttgccg ctgcaatccg
     4801 gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg
     4861 gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc cccatctctt
     4921 cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc
     4981 tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc tcaagtagtt
     5041 tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa
     5101 tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg
     5161 aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag
     5221 aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc
     5281 catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc
     5341 accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca
     5401 gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg
     5461 taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat
     5521 gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt
     5581 ccaaagctac gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg
     5641 catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt
     5701 gcaactggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc aacagcaacg
     5761 cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct
     5821 ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg
     5881 taagcttggt attaccttcg tctttgtcac tcacgatcag gaagaagcgc tcaccatgtc
     5941 agacaggatt gtggtgatgc gcgagggtcg tattgagcaa gatggtacac cgcgcgaaat
     6001 ttacgaagag ccgaaaaacc tgtttgttgc cggcttcatt ggcgaaatca atatgtttaa
     6061 cgccactgtc atcgaacgtc tagacgagca gcgcgtacgc gccaacgttg aaggccgcga
     6121 atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg
     6181 cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg
     6241 ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa
     6301 tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact ttgaccactc
     6361 tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga
     6421 agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt
     6481 ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca
     6541 gtttcgtaaa aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt
     6601 ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc
     6661 tgggctaccc gtttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc
     6721 tgtttctgct gattgttccc ttctggacca actcattgat tcgtatctac gggctgaaaa
     6781 ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgaca
     6841 caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc
     6901 tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg
     6961 aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgtatc attattccac
     7021 tgacgatgcc gggaattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt
     7081 tctatgtatc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg
     7141 tacagttcct caatattcgt gactggccat ttggtgcagc caccagcatt acgctgacta
     7201 tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtctgctg aataagaagg
     7261 tgaacgaatt agatgattaa tcctttatat tcaatttatt aggtttgttt ttgagtgatt
     7321 ttgtcgttta aggtaacggc gttatagatc ctatcaaaat caatcaattg cattctggtt
     7381 ttggagaaag atttttcgag agcttcggtc tttaactaaa actgattgcg tataatcgca
     7441 ataccccctc cccgactatc tgaatggtcg gggaggcact tcaagcacaa aatatcatcc
     7501 ctgctccgtt gattactcta caggtgaagt cggccactca atatcaggtg ctgctgatgt
     7561 atcaacacga ttcaacaaca cccgatactt tttccaggct tcccgcaatg aagtctcttc
     7621 ctcaattacg atctccagat ctgcagcatc ctgaagtggg gcgcaccacg caaacatcac
     7681 aaaatattgt cgttgtgatg gacctgtgca atagtgaatc acgctacgtt tctgtgtaca
     7741 acgtactgta gttttcttta cgatttatag ctgtactggt gagccatgag taatctgaat
     7801 ccatgcatgg cgtgtggtgc ctgttgtgca tttttctgcg tctcttttac tggtccgaag
     7861 ccgatgatgc tgaagcagtc ctctcctcta gcctcatccc tgtatagcac gcgtcaagta
     7921 agctgggata tcatttcgta gttttacaat atcaatatcc gaatcgatat gtttctggta
     7981 aaacttgtcc gcaagcaagt ttgctatggt atctgatgta cgatttttta catcccggct
     8041 cataccatca caaagagcat ccattttctt gccaaaagaa gcactcccca ttattacatg
     8101 attaatagaa ctttgtgctg ccttaacatc aagggttagt tgattctcct ggctcacaag
     8161 agaaaagact ctttgcctct cttcagtgga aatttttgca tttttttgaa aacagtaatt
     8221 atcaatctcc tggttgatca cccttgaggg atatactatg cgttcatctt ttatccaccc
     8281 atcaatatct ttagcactct gcctgttcac tgtatccagt atatttctgc taaatttgcg
     8341 aacagaaaag ctaatagtcc gttctccaat tgaacatttt acgacctcat cattcttccc
     8401 tcggaaagag ttcctaaagg agccttctct atggcaactt ataccaaaag agctgaaaga
     8461 cgtacctgaa gtattcatcg gcattataat tcctttttat aaaaaagcgt ttacaagatt
     8521 gcatgcttgt gtttattgta gccagaaaaa acatgcatgc aaataatatt ctattaaaag
     8581 ttgtattgtt tgagtcccat ctcatgatcg gtatgttgac tcttgttacc aatatagttc
     8641 acaaaatggg ttgaaaagga taataatatg ccagtcatat taaatttttc taatggaagt
     8701 gtattgccgg aaaatgagct ggaggcttta cgacatattg caagaagcaa tcagaatgac
     8761 actattacta taggaggccg taatatgagg cttcattata tccagtttat ggatggtttt
     8821 agtgtagagc ctattcctgg tggactttgg gaccatcttg gggcaagaga gtctcatcat
     8881 ctcgcagaca gcctggcaag acaacttaac ggaggtaaca cttttctgca ggcatacagc
     8941 ttatatctgg agcagaggca agctgccccg cttgtgcagg aaagcgtcat aaaaacacta
     9001 ctagatcgaa taaattcgaa tgcatttccc gttagtttac aagacttttc ctgtactgag
     9061 gagcatctta attgtccgat aacgctacat attcctgaga caggtgtttt tgtcagaaat
     9121 gctcgaaatt cagaaatatg tgcattatat gatcaggaag cattgactga acttatcctg
     9181 cgtaacgctc tccaccccct cagccgtgac ccctttgctc cagaaatgat tataagcaaa
     9241 gacaagtgtc attttaatat aacaaaacaa tgtttttacg cattacccat ataccccctt
     9301 caacaaaaca gtatttaaaa ataaaaacac aatataagaa gcatacaata agttaatcac
     9361 catataacaa acatatagcg aggagattat ttatgcccaa aatatcatca gttgtatcat
     9421 catgttacca tctgttcagt gaacatcaac aactttcaaa tgaaacaaca atgacgaact
     9481 ccgtctccag aagaattgtt cataaagaat atggtatatc tttaaaatcc gttcctgtat
     9541 ggttggctac agctaaaact ccccttgctc tactcaatgg cagacataca agaagtcact
     9601 catttattat tgcagggact ccaggaatgg gaagcaggag cggagcccaa tactatgcca
     9661 taaacagtga tgataaacgc tcccgcatag acattgactc tttattttta aaaaagttaa
     9721 ataatgcgcg aaatcaaaat aaatttccaa tcgatgtaaa agaaacggtt ataaaactac
     9781 aaggccaaaa atttacatgc attgaagatt ttcataaaaa gtataatgaa actaggctaa
     9841 aggccaatac caatatccaa caaaaacaaa ttgcagatga agtaaaatca cttacatatt
     9901 tgattccttc agaaaaaaaa gagatgtgga tatataaaaa caatggaaaa gataatgcaa
     9961 aaccaaactt aggagagcga gacgtaagaa tgttcgaaaa tattagtcct gatgatacag
    10021 ataagataac aggaaggaaa ttttcagagt taggtgagta tctttattca ggaaatgtaa
    10081 taaaactcag tcagttatca attcgttact tacccaatat caactcaatc tcattaatag
    10141 agacaaaaca gagtttgttg ctacatcgat tatattcaga tgaagtactt cagagaaatg
    10201 gaacacttat cccgacacca ctacatgaag aaaaatcaat tccagctgac aatataaaaa
    10261 caatgctcaa caacataccg acttacaaaa tgttaccgcc attcacagaa acacaaggta
    10321 attgttcttc tggcgcagcc acgtttttac gcaaatcagg cgccgaagaa aaagatattc
    10381 ttgcatgtag cccccgaaat tatgggctgc atcataacat aaaaacatgg gaccccttgg
    10441 ttagaaatta aggatccaaa tattatatat ttcatataaa gcaaggaaaa tattgcctga
    10501 ataattgttc aggcaatata tccttacatg gcactattat aataaactat taatataaaa
    10561 acacaccaac agaaaaaatt aagcatcact tgcaacaaag gcttcttttt ttgaatcaaa
    10621 gtgacattcg tcttttctca tgatcattga ttctgttata ggttctcgac tcagaggatg
    10681 agctccacca gtttcaacaa gttgcactaa cgcgtcctta tcatatagag agcatatctc
    10741 agcacctcgt gagtttctca tgaacacccc agtctcaggt gtgtccagcg ttatcgggca
    10801 tgtcaaaaat gactccgggc aagaaaagtg attagaatca ggtctaaata aacaagaatt
    10861 tattttttcc attaatattt tattacttgt tttattttct ctgtgttctg ccatgacact
    10921 ctgaaaatag ttattaacac tacttaaaaa atcgacacct ccatttaatt gcctttccag
    10981 agccacagcc cttctctcca ttcgatgctc acgccccaat agcctatcca gtaagcctcc
    11041 tctcaccggt tc
//
LOCUS       DAZFKM010000155        11000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFKM010000155 DAZFKM010000000
VERSION     DAZFKM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..859
                     /gene="adiY"
                     /locus_tag="ACHKAY_004336"
     CDS             98..859
                     /gene="adiY"
                     /locus_tag="ACHKAY_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO5702905.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            996..2333
                     /gene="adiC"
                     /locus_tag="ACHKAY_004337"
     CDS             996..2333
                     /gene="adiC"
                     /locus_tag="ACHKAY_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO5702906.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2437..4080
                     /gene="eptA"
                     /locus_tag="ACHKAY_004338"
     CDS             2437..4080
                     /gene="eptA"
                     /locus_tag="ACHKAY_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO5702907.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4077..4745
                     /gene="basR"
                     /locus_tag="ACHKAY_004339"
     CDS             4077..4745
                     /gene="basR"
                     /locus_tag="ACHKAY_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO5702908.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4746..5846
                     /gene="pmrB"
                     /locus_tag="ACHKAY_004340"
     CDS             4746..5846
                     /gene="pmrB"
                     /locus_tag="ACHKAY_004340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO5702909.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5822..5911)
                     /gene="pmrR"
                     /locus_tag="ACHKAY_004341"
     CDS             complement(5822..5911)
                     /gene="pmrR"
                     /locus_tag="ACHKAY_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO5702910.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6023..7525)
                     /gene="proP"
                     /locus_tag="ACHKAY_004342"
     CDS             complement(6023..7525)
                     /gene="proP"
                     /locus_tag="ACHKAY_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO5702911.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7789..8667)
                     /gene="rdcB"
                     /locus_tag="ACHKAY_004343"
     CDS             complement(7789..8667)
                     /gene="rdcB"
                     /locus_tag="ACHKAY_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO5702912.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8664..10892)
                     /gene="crfC"
                     /locus_tag="ACHKAY_004344"
     CDS             complement(8664..10892)
                     /gene="crfC"
                     /locus_tag="ACHKAY_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO5702913.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2749 a         2858 c         2708 g         2685 t
ORIGIN      
        1 tgcatgttta tattcatcat acaattatat aaccatttcc cggtatcgct ttgctttagc
       61 gagaactggt cttttatttg cactcaggag tacatgtatg aggatttgca gcgaccaacc
      121 ttgtattgtt ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat
      181 tagccttaaa gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc
      241 ctctcttaac aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg
      301 gtttctgaat aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccatact
      361 caccctgcca tgcctgacgc cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc
      421 cgcagaaact gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg
      481 ttttctcgac agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag
      541 tgacagcgtt tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt
      601 agccagttgt ttatgtctta gcccaagtct gttaaagaaa aagttgaaaa gcgaaaacac
      661 cagttatagc caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat
      721 ggacggtaaa aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt
      781 tatttctgtc tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag
      841 agaacgcact gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag
      901 cgataacccg gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt
      961 attgtcacga agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca
     1021 aagtgggctt aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt
     1081 ttctgttacc tgcaaacctg gcctctactg gcgggattgc catttatgga tggttggtga
     1141 cgattatcgg tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgatccaa
     1201 gtcctggtgg ttcttacgcg tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa
     1261 ccaacgtcct ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg
     1321 taggatattt aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct
     1381 gcgtcgtggt gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc
     1441 gtgtgcaggc cgttgccacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg
     1501 gctggttctg gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca
     1561 ccttcggtgc aattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa
     1621 gtgcctccgt tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca
     1681 ttggtggggt attgattgcc gccgtttgct atgtactttc taccaccgcg attatgggga
     1741 tgatccctaa tgccgcactg cgcgtttctg cttcgccatt cggtgatgcc gcacggatgg
     1801 cgttgggtga caccgccggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt
     1861 cactgggcgg ctggacgttg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac
     1921 tgttcccacc gatttttgcc cgtgtaaata aagcgggcac gccagtggcg gggctgatta
     1981 tcgtcggtat tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag
     2041 agttcggtct ggtttcttcc gtctcggtca tctttacact ggtgccatat ctttacacct
     2101 gtgcggcgtt actgctgctc ggtcacggtc actttggtaa agcacgcccg gcatatctgg
     2161 ctgttactac cattgccttc ctctactgca tttgggccgt ggtggggtcc ggggcgaaag
     2221 aggttatgtg gtcatttgtc accctgatgg taatcaccgc catgtatgcg ctgaattaca
     2281 accggctgca taaaaacccg tatcccttag atgtaccaat aagcaaagat taattcccct
     2341 taatccagca aacataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc
     2401 gagcatatgc gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga
     2461 ccctctttga atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg
     2521 aatattgcct tttttaaaca ggtgttgcag gcgctgccgc tggattcgct gcataacgta
     2581 ctggttttct tgtcgatgcc ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta
     2641 agctctttct tatggcttaa tcgaccactg gcctgcctgt ttattctggt tggcgcggct
     2701 gcacaatatt tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt
     2761 attgatacca ctccggcaga aagttatgcg ctgatgacac cgcaaatgtt attaacgctg
     2821 ggattcagcg gcgtgcttgc tgcgctgatt gcctgctgga taaaaatcaa acctgccacc
     2881 tcgcgtctgc gcagtgttct tttccgtgga gccaatattc tggtttctgt actactgatt
     2941 ttgctggtcg ccgcactgtt ttataaagac tacgcctcgt tgttccgcaa taacaaagag
     3001 ctggtgaaat ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat
     3061 cagcgactgg caaatctacc gctggtgcga attggtgaag acgcccaccg caacccgtta
     3121 atgcagaacg aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg
     3181 gagaacttct ccctcaacgg ctacccgcgt gaaactaacc cgcggctggc gaaagataac
     3241 gtggtctatt tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc
     3301 atgttctcgg atatgccgcg tgagcattac aaagaagagc tggcacagca ccaggaaggc
     3361 gtgctggata tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc
     3421 tgtaaaggtg cctgcgaccg cgtgcctcac cagaacgtca ccgcgctgaa tctgcctggt
     3481 cagtgcatca acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc
     3541 aataacctgc agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc
     3601 tattacaacc gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag
     3661 atccagacct gtagcaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc
     3721 gactatattg ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc
     3781 agcctggttt atctttctga ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac
     3841 ggtctgcctt atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg
     3901 tcggaggatt atcaaaaacg gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa
     3961 acgcaacact attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag
     4021 acgaagtatt accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga
     4081 aaattctgat tgttgaagac gatacgctgt tattgcaggg attgattctg gcggcgcaaa
     4141 ctgaaggcta cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg
     4201 ccggtcatta cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt
     4261 ttctcgcccg tatccggcag aaaaaataca ccctgccagt actgatcctc accgctcgcg
     4321 atacgctgac cgacaaaatc gctgggctgg atgtcggtgc cgatgactat ctggtgaagc
     4381 cttttgcgct ggaagagtta cacgcccgta tccgcgccct gctacgacgc cataataatc
     4441 agggcgaaag tgagctgatt gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat
     4501 ggatgggcgg tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa
     4561 tgctcaaagc aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg
     4621 acaatgaacc ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg
     4681 gcaaagcccg tatccgcacc gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa
     4741 actaattgaa tctgatgcat tttctgcgcc gaccaatatc gctgcgccaa cggctgatat
     4801 tgaccatcgg ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg
     4861 aaagtaccga gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc
     4921 gccacatcat gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta
     4981 tggtcagcct gacgctattt atctgctatc aggcggtgcg ccgcatcacc cgcccgctgg
     5041 cggagctgca aaaagagctg gaagcgcgca ctgccgacaa cttaacgccc attgccattc
     5101 acagcgccac cctcgaaatc gaagcggtgg tttcggcgtt aaacgatctg gtcagtcgcc
     5161 tgaccagcac gctggataac gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa
     5221 cgccactggc gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg
     5281 tagcaccgtt agtggcacga cttgatcaga tgatggagag cgtctcccag ctgctgcaac
     5341 tggcgcgtgc cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag
     5401 atgtcattct cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc
     5461 tgctattgcc agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga
     5521 tgttattgcg aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta
     5581 tgattaagct gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta
     5641 ttgatgagag taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg
     5701 gcgggattgg tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt
     5761 ttttcctgca aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag
     5821 atcagaacgt ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg
     5881 tagttaaact ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga
     5941 gtatgcgcgt cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc
     6001 acatcaggcc atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg
     6061 tacgtttcgc ctgcaaatcg gcaatctcgt ggtcaatatt atcgattttc tgctcgatat
     6121 tatcgtaatg ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg
     6181 cacctttcaa cggacgattt gccgtctctt tcatggttac gccggtgatt aaaccaatca
     6241 ccgccactac catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc
     6301 aggccgccag tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca
     6361 gcgcgctgta acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg
     6421 taaagcagtt aaggatcacc gccagcatca gtaacccggc aaaaatcagg ccgatgacgt
     6481 tactgttaat cagaataaac gccgggatcg ccaacacaaa cagggcaaca ctaccaagta
     6541 gcacaaacgg acgacggcca aaacggtcac tcagcaagcc catcaccggc tggacaaaca
     6601 gcataccgat cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt
     6661 tatgcgacaa ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa
     6721 ttaccagacc aatacatgtc aacaggctgc gccagtgttt ggtggcaatc tctttaaacg
     6781 agactttcgg gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcgacatgct
     6841 gctggaacgc cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg
     6901 gcagagcaat aaagaacgga atacgccagc cccagtcgag gaagttcgct tcgccgacaa
     6961 tggtcgaaat taacaccact acgccagcac ccagcacaaa cccggcaata gaaccgaagt
     7021 ccagccagct gcccataaag ccacgtttac ggtcaggtga gtattccgca acaaatatcg
     7081 acgccccggt atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca
     7141 gaatcggtgc ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac
     7201 tgatcgacat aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca
     7261 acataccaaa gaagagcccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa
     7321 gtgcagcaac catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt
     7381 aagcaacaaa accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg
     7441 cggtaatggc tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaattg
     7501 gttttacttt tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac
     7561 gccagaaaaa tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct
     7621 gtaacaagcg tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt
     7681 tgacgcctga attgggcagt taatgacctt tttaccctac cagtgtttaa atttgtgatc
     7741 aacttcacac atggatttcg tttaggactc attgatgtaa ctgattattt acgccaaatt
     7801 tacagagaag atttcctcga gcattgcccc cgccagacgc tgcgctgaac agagtcgtgg
     7861 catccccagc gcgacagttt gccagcgttg ggtcactgac cagcaaacgg gttgtttatc
     7921 tgccgtggtc cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac
     7981 ggtcgaagag gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact
     8041 ctctgccaga tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg
     8101 cgggcttccc agacgacagg catcgccaaa tgccccccaa cgcaactctt cgagaacgat
     8161 aaaacagcgc ccaggcaacg accaggcgtt atacgcccca gcacgccagc gggtaaaaat
     8221 ttgctcgctc tgatcacctg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt
     8281 ccagcggcaa cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg
     8341 gaaagcagag tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg
     8401 cgtcagtgcc agatgaccgg gatccagcat cgacgccagt ttttccacca atgtcagacg
     8461 caggctaagc ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacgcgcgc
     8521 aagaagttcc tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc
     8581 atcatcgccc tgcgccagat ccactaaaaa gcggggataa accgactcca gcacgcgacc
     8641 ggggccgtca agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca
     8701 tcgcgtaaca gctgggcgtc ggtgtaaatc cagccgtgag tcatcacagt ccgctgcaat
     8761 tgctgctgca tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt
     8821 aaacgcgtct gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct
     8881 gccgtaacag attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga
     8941 tcgacttctg cgcttaaagc tcgttgcaac acactgtaat caaccgtctc tgtcggggat
     9001 ttctcatttc cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca
     9061 acgcctaaac gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac
     9121 agctggcgag tgttgaagtg gggaactgcg ccgtgaaaaa caggaaaatg aaacccagga
     9181 cgaaaccctg actcgctcaa ttccattttg acttgttgct caatggggcg aataacatcg
     9241 gttaacaccc ggcaaagggt ggattccagc tcggcaaaac gcagcgtaaa gtcgcgactg
     9301 atggtgttct gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc
     9361 tccgaatcat cctgaaacaa cgcggctaac tgtgcattca ccgcatcttg ctgttgacgc
     9421 agaaagtggt tggcggaggc caaggccagc tcgatttcat gtttaatctc gccgctcacc
     9481 tgcgcctgat tgagttgcaa tagctgcaaa ctttcttcga cctgatggat attttgccgc
     9541 aattgttcac aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc
     9601 tgcgcgtaat tcaacagttt atgcgcggca gatcgcagag catacaacga ggcgttagcg
     9661 taagcggcat gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga
     9721 tcggcagcat gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg
     9781 agcgcggcat gggcaaaatc ttccacccag cgttgttgct ctggcggtgg taacttaccg
     9841 ttgttggcta actcatggcg cgcccgattc gccaggtagc cccacatcga cgacaccgga
     9901 aatatctgct gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc
     9961 tggtcggcgt cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc
    10021 ggcaccgatt gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc
    10081 agttgcgtat aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc
    10141 attttttgca gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac
    10201 tgaccgggat aactttccag ccccgccaga tggacaaact ccacttcaat cacgggaata
    10261 tgctcaatgg cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc
    10321 actaaatcat tcaaactttt cagacaatga aaaataggct gggcacccag ataatatttt
    10381 tcgaaagcga cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc
    10441 agcacatcgg tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa tttttgaatt
    10501 aaacggtcga ttggcgcgac atgtgaaaaa tgcagtaccg gttctttttg cccgggcgta
    10561 tggcgaataa gcgtcggcag cgcagtcatt gggcgattac gattaggcaa aacctccgta
    10621 ccaacaatgg cattaatggt ggttgatttc cctgctttca tggtaccgac aatcgccagc
    10681 accatttcca gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgttcggcg
    10741 atattaggct gactccaggg taaagccagt tgtggcgcat cgtctccggg tacagagaga
    10801 ggcatttttt ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc
    10861 tgacttaact catacagggt ctgtgtgtac atagaaaatt cttccttaaa gcaaattttg
    10921 ttacttttat ttagccagat tgtttttgag ttctgttttc ggcttttata attactgcaa
    10981 gaaataattt tatatttagc
//
LOCUS       DAZFKM010000156        10981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFKM010000156 DAZFKM010000000
VERSION     DAZFKM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHKAY_004345"
     CDS             <1..75
                     /locus_tag="ACHKAY_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5702914.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHKAY_004346"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHKAY_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5702915.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1543
                     /locus_tag="ACHKAY_004347"
     CDS             863..1543
                     /locus_tag="ACHKAY_004347"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5702916.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1540..1722
                     /locus_tag="ACHKAY_004348"
     CDS             1540..1722
                     /locus_tag="ACHKAY_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5702917.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            1695..1886
                     /locus_tag="ACHKAY_004349"
     CDS             1695..1886
                     /locus_tag="ACHKAY_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5702918.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            1963..2178
                     /locus_tag="ACHKAY_004350"
     CDS             1963..2178
                     /locus_tag="ACHKAY_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5702919.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2495
                     /locus_tag="ACHKAY_004351"
     CDS             2277..2495
                     /locus_tag="ACHKAY_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5702920.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            2543..2821
                     /locus_tag="ACHKAY_004352"
     CDS             2543..2821
                     /locus_tag="ACHKAY_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5702921.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            2793..2858
                     /locus_tag="ACHKAY_004353"
                     /pseudo
     CDS             2793..2858
                     /locus_tag="ACHKAY_004353"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3020..4183
                     /gene="intD"
                     /locus_tag="ACHKAY_004354"
     CDS             3020..4183
                     /gene="intD"
                     /locus_tag="ACHKAY_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO5702922.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4199..4275)
                     /locus_tag="ACHKAY_004355"
     tRNA            complement(4199..4275)
                     /locus_tag="ACHKAY_004355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4239..4241),aa:Arg,seq:tct)
     gene            4518..5150
                     /gene="fimZ"
                     /locus_tag="ACHKAY_004356"
     CDS             4518..5150
                     /gene="fimZ"
                     /locus_tag="ACHKAY_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO5702923.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(5153..5668)
                     /gene="sfmF"
                     /locus_tag="ACHKAY_004357"
     CDS             complement(5153..5668)
                     /gene="sfmF"
                     /locus_tag="ACHKAY_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO5702924.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(5679..6656)
                     /gene="fimH"
                     /locus_tag="ACHKAY_004358"
     CDS             complement(5679..6656)
                     /gene="fimH"
                     /locus_tag="ACHKAY_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO5702925.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            complement(6698..9307)
                     /gene="sfmD"
                     /locus_tag="ACHKAY_004359"
     CDS             complement(6698..9307)
                     /gene="sfmD"
                     /locus_tag="ACHKAY_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO5702926.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFEGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(9338..10030)
                     /gene="fimC"
                     /locus_tag="ACHKAY_004360"
     CDS             complement(9338..10030)
                     /gene="fimC"
                     /locus_tag="ACHKAY_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO5702927.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(10250..10792)
                     /gene="fimA"
                     /locus_tag="ACHKAY_004361"
     CDS             complement(10250..10792)
                     /gene="fimA"
                     /locus_tag="ACHKAY_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO5702928.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         3172 a         2564 c         2513 g         2732 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggcaggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gttgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcattg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gtacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gaatggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacggcata
      541 aagccatgat tcagtgtgcc cgtctggcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aatactgcat acactgcaga acgtcagcca gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg agattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgcg
      781 catcgtcaga actgacacag gccgaagcag tgaaagctct tggattcctg aaacagaaag
      841 ccactgagca gaaggtggca gcatgatacc ggacattatc ctgcagcgta ccgggatcga
      901 cgtgagagct gtcgaacagg gggatgatgc atggcacaaa ttacggctcg gcgtcatcac
      961 cgcttcagaa gttcacaacg tgatagcaaa gccccgctca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taatgctaag gcgctggcct ggggaaaaca gtacgagaac gacgccagaa ccctgtttga
     1141 attcacttcc ggcgtgaata ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac tgaaatgccc
     1261 gtttacctcc cgggatttca tgaaattccg gctcggtggt ttcgaggcaa taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacgcga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatttgggga gcaatggcga tgacgcatcc tcacgataat
     1561 atccgggtag gcgcgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatcagaaa tccactgaaa gcacagcggc tggctgagaa gataaataat
     1681 aaacgggagg cggtatgcac aaagcatctc ctgttgagtt aagaacgagt attgagatgg
     1741 cacatagcct tgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggtg gcgaaagcag
     1861 aagcagatga gagagaccag gtatgacaac cactgaatgc atttttctgg cagcgggctt
     1921 catattctgt gtgcttatgc ttgccgacat ggggcttgtt caatgacacc tcagcaagaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcat
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac taaagaagca ccgcgaaacg
     2101 gtaacgctga tgggattcac accgactcat ttaagcctgg cgatcggcat gttaaacggc
     2161 gtctttaagg aacggtgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcatcctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaatcg aagaattaca gcgcaataca gcaataaaaa
     2341 tgcgtcgtca gaactaccag actgtatccg caactcattg ttgtgagtgt ggcgatccga
     2401 tagatgagcg aagacgcctg gctgttcagg gttgtcggac ttgtgcaagt tgccaggagg
     2461 agatcgaact taagaacaaa caatggggac tgtgatggcc tcaaagcagc aaatttcaac
     2521 atcgtccaac tgaggtgtaa aaatgttcag aatcattttt cctaacacct ggtacgtcga
     2581 ccaccacggc actccctgca aaatcctgcg ttctacccac aacaaagttc actacatccg
     2641 aaaaggcaga acatgtatcg ccagcatgtt ccgctttaat catgactttg aacctgtgaa
     2701 taaagctgat gcagatcgga tagcagaaga gatcgaaacg gcagaacaca ttaagaagtt
     2761 acgtgacatg cgttcaaaaa gcagaggtaa ccatggaatc atacagcctc acactcgatg
     2821 aggcctgtca gtttcttaag atatccagac caaccgccac caactggata cgaacaggcc
     2881 gcctacaggc aacacgtaaa gatccaacca agccaaaatc tccttacctc acaacacggc
     2941 aagcctgcat tgcggcgctt cagtctccgc tgcatactgt ccaggtgagc gcgggtgatg
     3001 gcataacaga ggaaagaaaa tgtcactctt ccgcagaaat gaaatatggt atgcctcgta
     3061 ttcgctcccg ggcgggaaac gaattaagga atctcttggc acaaaggaca agcggcaagc
     3121 tcaggagttg cacgacaagc gaaaagcaga actctggcga gtagaaaagc taggggattt
     3181 acctgatgtc acttttgaag aggcctgcct aagatggctt gaggaaaaag ctgataaaaa
     3241 atctctcgat tcagataaaa gccggattga gttctggctt gaacattttg agggtataag
     3301 gcttaaagat atctcggagg caaagattta ctctgctgta agcagaatgc ataacagaaa
     3361 gacgaaagaa atatggaaac agaaagttca ggccgccatc aggaaaggta aagaaccgcc
     3421 tgtttatgaa ccaaagccag tatcaactca gacaaaggca aagcatcttg ccatgataaa
     3481 ggccattctc cgtgctgcag aacgcgactg gaagtggctg gaaaaagcgc ctgtcatcaa
     3541 gataccagcg gtcagaaaca agcgagtcag atggctggaa aaggaggaag caaaacgcct
     3601 tattgatgag tgccccgaac cactgaaatc tgtcgtcaag tttgcgctgg caactggtct
     3661 gagaaagtcg aacatcataa atctggaatg gcaacaaatc gacatgcagc gacgagttgc
     3721 ctgggtgaat ccagaagaga gcaaatcaaa ccgcgccatt ggtgtggcgc tgaacgatac
     3781 cgcctgtaaa gtgttgcgtg atcaaatagg caagcatcac aaatgggtgt ttgtacatac
     3841 caaggcggct aagcgagcag atggaacatc aacgcctgcg gtcaggaaga tgcgcatcga
     3901 cagcaagaca tcatggctat cagcttgtcg tcgtgcagga attgaagatt tccgtttcca
     3961 tgacctcaga cacacctggg caagctggct gattcagtca ggcgtcccat tatcagtgct
     4021 tcaggaaatg ggcggatggg agtccataga aatggttcgt aggtatgctc accttgcgcc
     4081 taatcatttg acagagcatg cgaggaaaat agacgacatt tttggtgata atgtcccaaa
     4141 tatgtcccac tctggaatta tggaggatat aaagaaggcg taactgattg aattgtaatg
     4201 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
     4261 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
     4321 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
     4381 ttctgattta tttcgctata gcggcaaaca aacacacacc gctgcgcgtc tgaatcaaga
     4441 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
     4501 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
     4561 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
     4621 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
     4681 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
     4741 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
     4801 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
     4861 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata
     4921 taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag
     4981 tgaccatatt acgttatctg gttagcggat tatctaataa agaaattgcc gataagttat
     5041 tacttagcaa taaaacagtt agtgcgcata aatctaatat ttatggcaag ctaggtttgc
     5101 attcaattgt agagcttatc gactacgcca aattatacga attaatataa tattaattat
     5161 aattgatcat aaatatcgca tccgctttcg ccacaccagg ccgaacaccg ctggctaacg
     5221 ctcgataatt ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg
     5281 ggctcgcctc ccccagtgcg agccgggagc gatcgctatt acgtaattcg atggcgacgg
     5341 tttgtgccat tgcgggatca tccagagcca gcaggttggt atcggatgcc ggcgttcccg
     5401 taaataaaat cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggacga
     5461 gtgccgtggt atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg
     5521 tcttatcaat atccgctgtg tttacggtac aggagaaatc aacaacgtta ccgtgcaaat
     5581 tgatattaat cgttcctaaa gggtcagctg cccatccaat ggaactccac agtaacccgc
     5641 agaaacagct aaagagtact cttctcattc tccgtacctc attgataatc gacgcgtaaa
     5701 taccccagcg cgctaaacgg cccctcggtc ggtttttgac cggtaatact gataggccag
     5761 gcgcgaagtg tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
     5821 agatcgttag gcgtaatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
     5881 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
     5941 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacgcca
     6001 gctggtcgat tccccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
     6061 aattcaacaa tctgcccggc atttaattcg cagttttgcg gcacttcaac ccggccacca
     6121 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
     6181 gtaaacatca cctgtctggg gatctccacc atgttaataa agggacgaat aacctttaaa
     6241 cgaaatatta aacgagaatc catcaccccg aatggaaggt tttgcgaaac gttaggatct
     6301 gttcccatcc gaatataatt ttgcgggggg aactgaatat ccatcacact atcaaccaga
     6361 ctcatcgcac ctaatagata atcatgtaat tgcatatatt tataattatc gatagtttcc
     6421 tgaacaataa agttggtgac ataactacgg tatgtataat tcaccagcgt accgggtgga
     6481 caaattgctg agacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc
     6541 tggttattgg tgctattaaa gatatttgag agatcatagt gttcatcttc tgctacgcca
     6601 gtactatttg cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccatcgcc
     6661 aataataatc tgcagattat cttattgata atttccttta tgagccagga tgtgtgcaaa
     6721 ctgccgaaat aacagtgacg gcttgctgta agctttgctt cggcaataca tagtgcgcaa
     6781 tgcagcgtga gttcgcgccg tctccccact gaaccagtaa ttcaccagac aatggcgcac
     6841 cacttaaata aacttgcccg tcatcaccca ccatactggt tattccggta ctgttttccc
     6901 gtaccagtga tccaaacggc accggttttc tgccctgggt aacggtaatg agcgcccgca
     6961 cgcctatgcg ggtatcaaaa ttggcacgaa ccaacgcgcc ttgcgtcggc acaacgctgc
     7021 taatattttt ttcaacatcg atggaattcc ccatcgtatt ggtatcaagc gcgatacggt
     7081 tataccgata aaccgtggca tacggcatca ccgcatagcc gcgccagtcg gttaaaatgc
     7141 cagtttgatt ttcaatgcgt acaccgcctg cgccaggcgc tttaatcaaa acattggtat
     7201 cccctaaagg ctggctcagc gttatgccat tttcatgccc gaccacaccg ccagacagct
     7261 gccagttaac gtcatgttga tcgcggtcgt agttataccc gacccccagc gtaccgtaag
     7321 cggcctgcca gtttgccgtg gcgctgcccg tatacccatt attcgaggta tcaccctggc
     7381 ttacgtgata actcaggttg tggccttcca gtaaggtccc acctacacct gccagccagc
     7441 tattttgccc gttgctgtta cggttggcgt taaaggaggc ataagcgcgg tcgagcgcat
     7501 tttcccgggt gtagcgacgt ttggtcagaa cattgaaagg cactgaaaca ttaagtccga
     7561 caatacgttc gttatcgggg atccctacag attcattcca cgaaaatgag agtgaataac
     7621 tgatgccaac ccagctgctg gtatacccca cctgatacca ggtatctgaa tccgaagtat
     7681 tccagtattt ttgatgggta ccagagatat aaagcgagcc aaagtcatta agtgattgtg
     7741 aaatatttaa ctgcaaacgg tctttacggc taaagcgtaa attgtggtaa ttcacgatta
     7801 tcggttcatc gcgatgctca ccgtcgtaat cgtaatcata ttcgtacccc tccattcgac
     7861 gatacgcaac atcatcaagg gtataaaaac cttgtgtcga atagcggtaa cccattaact
     7921 gaaaattggt gccgaaggtg ttcatcgatt tcgcatagag gaagcgaatg gaatccccct
     7981 catgacgact gtcgtcggct aactgactgc gcgcatgggt cacatccagc gacactgtgc
     8041 cccagttccc gaggttgcgc cccagcccta ataaaaaggc ggtgtaattg gcagataatt
     8101 gcgtcccgcc gtaggcagta aattcctgtg gtaaaccgcc gagtgccgta ccctgaaaaa
     8161 agaaaggcga tgattgctga ctattaccgc tacgaaaatc gcccgccgtc aggtcaaatt
     8221 tgaaacgccc ttcgcgttgt aaaattggca ctgttgaata cggaattgtg taattctgct
     8281 gattaccatc gcgctcgtcg atggtgacgt ccagatcgcc atttgaagat gtcgggtgca
     8341 aatctgtaat ttcaaaagcg ccgggggaaa catagctttg atagataata aaaccatttt
     8401 gccgaatcgt tagctgggcc gccgtacggg caatcccacg tactgttggg gcaaaccctt
     8461 gctggctatc aggatacata ttatcggaag agtaaagccg tataccacga aatccaacac
     8521 cctcgaaaat atcacttcct gtattgccgt ctcccattac cagttcactt ttcagcggaa
     8581 taatggcgcg ctgtacccag gtgccaatat tattccactg ttctgaatga tatccatttc
     8641 cgcgaaaata gttccaggaa ccattgttgc gtaaacgcca cgggccaata ttaatcccgc
     8701 tgagctcact aaaaaaatag ctatcgttac cattacctct gttaccggtg aaattataat
     8761 tcaggagtaa agcaggaata ccttcatccc actcttcagg cggaatgtaa ccgtgagcgc
     8821 tactcaacaa cgctatctga ggaatagtga tgttcaggcg catcgctgca aaatcaaagt
     8881 taattgtggc atcaggtaca gctttcagta aattgatgca tttattgttt tgctgctggg
     8941 ctaactcggg aaatgcactg ctattaaggc taattcgttc aaggagtacc tggttaaaac
     9001 agggcattaa cccacctgat ttatcacctg tattttctgt tgtcgattca aatacgatat
     9061 cctgtgaacc aatgaactca tcattacgcc agagatctac ccgatacacg cccgcaggtt
     9121 gatgattacc tctctcaaag cgcgataaat cagcaacgga tgtgccattc tctaataaaa
     9181 atgccgggtt gaaataactt tccgcatagc ttaaaaagga gggtaaaatg gcaagagatg
     9241 aataacaaat tccggccaga caccaggtat acctctgcgg aatatccgta gtagtgggta
     9301 ttttcataat tagctctgat attgtcagat gattcggtta gtttaagttc acttctctga
     9361 ccggggttac tgaaccataa tcattaacgg tctgaaattt cagctttccc tgcactcctg
     9421 agggtaaggg aatttgtgaa ttaactctgg gtgcagccat agcattcccc aacttttggc
     9481 tgcccacttg taagttaacc agagtgacat aaaaaggtga gggattatga acattgatat
     9541 agttaccgga tcgcgaaaac ttgagtgtgt ccggtgcttc tgcgggtaat tcttgtaatt
     9601 gaattagacg gagaaataat ttcatgcgcg ataaaatcgc cagttgcaaa acattcctgc
     9661 cgttcaatgc atttttatct accgaaggga tcgtcttaac attcatccag aacagagact
     9721 cacgatctgc tgccagcggt ggaccggtgt aaataatacg caaggtattt tcgcttttgg
     9781 gttcactaac aaacagtggc ggtgtaatga tgaatgactt ttcttttaca ccgctgctgt
     9841 tttcaatcca cgaattgacc agaaagcgct cattggtatg gctatttcta atccataccg
     9901 cagtctgttt agcatcagcg ggataaataa tacgcgtggc acctaatgcg atccctccgg
     9961 cagcatgggc gctggccgaa atgattaaat aaaatataat gagcatcaat aactttattt
    10021 tagtcatcat agttaatacg ccttgtgtat tcctgacggc aaaaataaga aatataaaga
    10081 aatgattgct ttggtttctt acaacacatc attccctttt catttaataa agcggcatga
    10141 tacaaagtga acatgatttc gatttacatt gccaccatac cgcttcagac aaccggtaca
    10201 ttactttgtt ataagacgtg atatataatt caaaacaacg tggttttgat tattcgtatc
    10261 tcataataaa agtcgcgtca gcatttgctt gccctgctga tgcacttgta ccggtgcctt
    10321 tataacgtgc agaaaaatga agaacgttgg tcccggggat caagttctgg ttggttgaga
    10381 agctattccc atcaggtttg agaattgcgg atgtattatc aagtatttca ataccgacac
    10441 ccgttgctgt tgttgtattg gtactggatg caatggccaa taaattatca ttgattgcat
    10501 cagcctgacc agaaaatgca acggcagcat tagcggcaac aacaggatcg cagtcgttca
    10561 actgaatggt gaatggaatt aatgcagagg tattaccaac agcattgaaa atatcggtac
    10621 gatattgacc gagtgtgaca acctggtctg ccgagtcagt attcactgaa caggcagcat
    10681 tcaccagttc gccttcaaag tgaattgtac cgccatctac aacggcagca taactacccg
    10741 tagcggcgaa tagtgcggca gccagcgcag acgaaataaa tcttaatttc atatatattc
    10801 cttcaatctc atttatcgac tccacatccg tatataaccg attactttat ttaagacact
    10861 gatagtagta aaattccttt ttatcctcta agaatgtctt aattgaaaat atgcactcta
    10921 ttctaaaaaa tagagagccc cgttagatga atactttcgc gcaaaatata ttcaacacaa
    10981 a
//
LOCUS       DAZFKM010000157        10895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFKM010000157 DAZFKM010000000
VERSION     DAZFKM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..1794)
                     /gene="glnS"
                     /locus_tag="ACHKAY_004362"
     CDS             complement(130..1794)
                     /gene="glnS"
                     /locus_tag="ACHKAY_004362"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO5702929.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1997..3943)
                     /gene="nagE"
                     /locus_tag="ACHKAY_004363"
     CDS             complement(1997..3943)
                     /gene="nagE"
                     /locus_tag="ACHKAY_004363"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO5702930.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4276..5076
                     /gene="nagB"
                     /locus_tag="ACHKAY_004364"
     CDS             4276..5076
                     /gene="nagB"
                     /locus_tag="ACHKAY_004364"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO5702931.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5136..6284
                     /gene="nagA"
                     /locus_tag="ACHKAY_004365"
     CDS             5136..6284
                     /gene="nagA"
                     /locus_tag="ACHKAY_004365"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO5702932.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6293..7513
                     /gene="nagC"
                     /locus_tag="ACHKAY_004366"
                     /gene_synonym="nagR"
     CDS             6293..7513
                     /gene="nagC"
                     /locus_tag="ACHKAY_004366"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO5702933.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7561..8313
                     /gene="nagD"
                     /locus_tag="ACHKAY_004367"
     CDS             7561..8313
                     /gene="nagD"
                     /locus_tag="ACHKAY_004367"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO5702934.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8570..10234
                     /gene="asnB"
                     /locus_tag="ACHKAY_004368"
     CDS             8570..10234
                     /gene="asnB"
                     /locus_tag="ACHKAY_004368"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO5702935.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10615..10691
                     /locus_tag="ACHKAY_004369"
     tRNA            10615..10691
                     /locus_tag="ACHKAY_004369"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10649..10651,aa:Met,seq:cat)
     gene            10700..10784
                     /locus_tag="ACHKAY_004370"
     tRNA            10700..10784
                     /locus_tag="ACHKAY_004370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10734..10736,aa:Leu,seq:tag)
     gene            10808..10882
                     /locus_tag="ACHKAY_004371"
     tRNA            10808..10882
                     /locus_tag="ACHKAY_004371"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10840..10842,aa:Gln,seq:ttg)
BASE COUNT         2703 a         2821 c         2781 g         2590 t
ORIGIN      
        1 aaattgcgaa gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagtgcggcg
       61 tttgggattg tttgctattt cttgccggat gcgacgtaaa tgcctcaccc ggcctggtag
      121 aacttaaaat tactcgccta ctttcgccca ggtatcacgc agcccaacgg tgcggttaaa
      181 taccggtttt tccgctgtgg aatggcggct gtcgaggcag aagtaacctt cacgctcaaa
      241 ctggaatgct ttacccgcaa ccgcatcttt cagcgacggt tcagcaaagc cctgtttgat
      301 caccagcgat tccgggttaa tcaccgacag gaaatcatcc gcagcaccag ggttcggcac
      361 gctgaacaga cggtcgtaca gacggatttc aaccggcagc gcatgtgccg cgctcaccca
      421 gtgaataaca cctttgactt tacgaccatc tgccggatct ttgcttaagg tgtcggcgtc
      481 ataagtacag aagatggtgg tgatattgcc ttcggcatct ttctccacgc gttcagcctt
      541 aatgacgtaa gcattacgca gacgcacttc tttacccagc accagacgtt tgtactgctt
      601 gttagcttct tcgcggaaat cggcgcgatc gatccaaatc tcaccgctaa acggcacctg
      661 acgactcccc atttccggtt tgttcggatg gttcggcatg gtgaccattt cgccttcgcc
      721 ctgatagttt tcgataacca gtttcaccgg atcgataacc gccattgcgc gcggcgcatt
      781 ttcgttgaga tcttcacgga tgcaggattc cagcgatgcc atctcaatgg tgttgtcctg
      841 cttggtcacg ccgatgcgtt tgcagaactc acgaatagaa gcagcagtgt aaccacgacg
      901 acgcagaccg gaaatggtcg gcatacgcgg gtcatcccag ccttcaacgt gcttgtcggt
      961 caccagcagg ttcagcttac gcttggacat cacagtgtat tccagattca ggcgcgagaa
     1021 ttcgtactgg cgcgggtgaa caggaatggt gatgttgtcc agcacccagt cgtacaggcg
     1081 acggttgtcc tggaactcaa gcgtacacag agagtgcgta ataccttcca gggcatcgct
     1141 gatgcagtgg gtgaagtcgt acatcgggta gatgcaccac ttgttgccag tctggtggtg
     1201 ttcagcaaat ttaatacggt acagcaccgg atcgcgcatc acgataaacg gtgaagccat
     1261 gtcgattttc gcacgcaggc aggctttacc ttcttcaaaa ccaccggcac gcattttttc
     1321 gaacagcgcc aggttctctt caacgctgcg gtcgcggtac gggctgtttt tacccggttg
     1381 cgtcagggtg ccgcggtatt cgcggatctg ttccggcgtc agttcatcaa cgtacgccag
     1441 gcctttattg atcagttcga tcgcataggc gtggagctga tcaaaataat cggaggagta
     1501 acggacgtta ccagaccagt gaaaacctaa ccactctacg tcgtttttga tcgactcaac
     1561 atactcgata tcttctttta ccgggttagt gtcgtcgaaa cgcaggttgc actggccttt
     1621 atagtcctgg gcgatcccga agttcaggca gatagatttc gcatggccaa tatgcagata
     1681 gccattcggc tccggcggga aacgggtgtg tactgtggtg tgcttaccac tggccagatc
     1741 ttcatcgatg atctgacgga taaagttagt cgggcgggct tctgcctcac tcatcgtgga
     1801 ttcctcaaag cgtaaacaac gtataacggc gtatgatctt ataagcggga caggctgaca
     1861 actgttagtt ttttaaaatt gataatctaa caaggattat ggggatgatt gatgaaaaaa
     1921 aaagcggcag agaagatctc caccgcttgt cattgttgga tgcgacgctc aagcgtcgca
     1981 tcaggcataa agcagattac tttttgattt catacagcgg tgtttgaccc gccacaacat
     2041 ggccctgagc tttaatgatc aagccactga aatcgtcgat attgctgcaa accaccgggc
     2101 taatcatcga gcgggcgtta gcgttcaggt aatccagatc catttccaga atcggttgcc
     2161 ctgcgcttac ctgcgcaccc tcttccacca gacgtttaaa gcctttacct tccagcgcta
     2221 cggtgtcgat acccatatgg acgacgatct ccgcgccttt ttcggtttcc aggcagaacg
     2281 cgtggttggt gttgaagatt ttcacgattg tccctgcggc tggtgatacg acgattttat
     2341 ctgtcggttt caccgccaca ccgtcaccca ccgctttgct ggcgaatgct tcgtcaggaa
     2401 cctgatccag tgccacgaca tcaccggtaa tcggcgatac cagctccgcg atagataccg
     2461 cgtttggtac agcctgcggt tttgctacag gcgcggcagt tgccggagtt gcttcagctg
     2521 acgcagcggc taccggacca cgggcaacga ctttcttcat cgcatcgccg atggattctg
     2581 ctttcgcgcc aacaatcacc tgaatagtct gtttgttcag tttcactaca ccagaagcac
     2641 ccagacgttt acacatcgta tcgttgacgc gggcagagtc agccacggtc aggcgcagac
     2701 gggtgataca ggcgtcaatt gctttcaggt tgtcagtgcc gccaaccgca gcaatatagt
     2761 tggtcgccag ttgattgagg ccttcttcag tgttgctgtt ggcttcttca gtaacgatct
     2821 cgtcttcttt atcttcacga cccggcgttt tcaggttgaa catgcggata accaaactga
     2881 acaccacgaa gtagatagcg aagaagacaa cacccataac cagcagcatc cagacgttct
     2941 ggctggcggc cggcaggtta tacatcaacg cgtagtcgat agcccccgct gagaaagaga
     3001 agccagcatg gatacccagc agtgttgcca caaacaggct gataccggtc agcagtgcgt
     3061 gcaggaggta cagcagcggt gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac
     3121 cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc gccaaccatc ggacgacgct
     3181 ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg cagaccgaac atcataatcg
     3241 ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc ggcatagaag cggttgatat
     3301 caccgtggaa aaccgtaccc gccgcgttgg tgaattcacc aatctggaac caggcgatgg
     3361 tgttcagcac ctgatgcaga ccggttggga tcagcagacg gttgatgaaa ccaaagatac
     3421 cggaacccag cgcgcccgca gaaacgatcc actcgcctcc tgcatggata gcgtgctgta
     3481 ccggcggcca gacgtaacca aaaatggccg ccagcaccag gcagaagaag ccggtggcaa
     3541 tcggcacaaa gcgtttgccg ccgaagaaac tcaggaagtc cggcagttta atatcggacc
     3601 aacggttata ggctgcgcca ccaaccagac cggtaatgat acccgccagt acacccatgt
     3661 taatttctgg gttgatggtc accatcgctt tggttaacac aaagtaacct accgcacccg
     3721 ccagcgccgc cgcacctgcg ctgtctttcg accagctgga tgccacaccg atggcgaaga
     3781 ttaatgcgag gttatcaaaa atcgcaccgc ccgcctgggc aataaacgca acgttaagta
     3841 aatctggctg accgaatcgc agcaacagtg ccgccaccgg cagcaccgcg atagggagct
     3901 gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt catcttattc cccctacgag
     3961 aaccctattt ggctcgttta aagccgtatt tttattttgc tgcaaattgt actgccgatg
     4021 ttctgtaatc agattgttag atcatctgct acagagtgtg tgaaaattta attcgtatcg
     4081 caaattaaac gcgtgtcttt tgtgagtttt gtcaccaaat atcgttatca tcactccctt
     4141 ttactggcta aagcagaaaa cttattttat cattcaaaaa atcaggtcgg attgacgcct
     4201 gtctgcgcaa atccaggtta cgcttaaaga tgcctaatcc gccaacggct tacattttac
     4261 ttattgaggt gaataatgag actgatcccc ctgactaccg ctgaacaggt cggcaaatgg
     4321 gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac cgactgccga tcgtccgttt
     4381 gtactgggcc tgccgactgg cggcacgccg atgaccacct ataaagcgtt agtcgaaatg
     4441 cataaagcag gccaggtcag ctttaagcac gttgtcacct tcaacatgga cgaatatgtc
     4501 ggtctgccga aagagcatcc ggaaagctac tacagcttta tgcaccgtaa tttcttcgat
     4561 cacgttgata ttccagcaga aaacatcaac cttctcaacg gcaatgcccc ggatatcgac
     4621 gccgagtgcc gccagtatga agaaaaaatc cgttcttacg gaaaaattca tctgtttatg
     4681 ggcggtgtag gtaacgacgg tcatattgca tttaacgaac cggcgtcttc tctggcttct
     4741 cgtactcgta tcaaaaccct gactcatgac actcgcgtcg cgaactctcg tttctttgat
     4801 aacgatgtca atcaggtgcc aaaatatgcc ctgactgtcg gtgttggtac attgctggat
     4861 gccgaagaag tgatgattct ggtgctgggt agccagaaag cactggcact gcaggctgca
     4921 gttgaaggtt gcgtgaacca catgtggacc atcagctgtc tgcaactgca tccaaaagcg
     4981 atcatggtgt gcgatgaacc ttccaccatg gagctgaaag ttaagacttt aagatatttc
     5041 aatgaattag aagcagaaaa tatcaaaggt ctgtaattgt tatccctgcc ctggctcctt
     5101 gctcagggca atatttttta aaatcggggg tcagaatgta tgcattaacc cagggccgga
     5161 tctttaccgg ccacgaattt cttgatgacc acgcggttgt tatcgctgat ggcctgatta
     5221 aaagcgtctg tccggtagcg gaactgccgc cagagatcga acaacgttca ctgaacgggg
     5281 ccattctctc ccccggtttt atcgatgtgc agttaaacgg ctgcggcggc gtacagttta
     5341 acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat catgcagaaa gccaatgaga
     5401 aatcaggctg tactaactat ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac
     5461 agggcgtgcg cgttatgcgc gagtacctgg caaaacatcc gaatcaggcg ttaggtctgc
     5521 atctggaagg tccgtggctg aatctggtaa aaaaaggcac ccataatccg aattttgtgc
     5581 gtaagcctga tgccgcgctg gtcgatttcc tgtgtgaaaa cgccgacgtc attaccaaag
     5641 tgaccctggc accggaaatg gttcctgcgg aagtcatcag caaactggca aatgccggga
     5701 ttgtggtttc tgccggtcac tccaacgcga cgttgaaaga agcaaaagcc ggtttccgcg
     5761 cggggattac ctttgccacc catctgtaca acgcgatgcc gtatattacc ggtcgtgaac
     5821 cgggcctggc gggcgcgatc ctcgacgaag ctgacattta ttgcggtatt attgctgatg
     5881 gcctgcatgt tgattacgcc aacattcgta acgctaaacg tctgaaaggc gacaaactgt
     5941 gtctggttac cgacgccacc gcgccagcag gtgccaacat tgaacagttc atttttgcgg
     6001 gtaaaacaat atactaccgt aacggacttt gtgtggatga gaacggtacg ttaagcggtt
     6061 catccttaac catgattgaa ggcgtgcgta atctggtcga acattgcggt atcgcactgg
     6121 atgaagtgct gcgtatggcg acgctctatc cggcgcgtgc gattggcgtt gagaaacgtc
     6181 tcggcacact cgccgcaggt aaagtagcca acctgaccgc attcacacct gattttaaaa
     6241 tcaccaagac catcgttaac ggtaacgagg tcgtaactca ataagagaaa gtatgacacc
     6301 aggcggacaa gctcagatag gtaatgttga tctcgtaaaa cagcttaaca gcgcggcggt
     6361 ttatcgcctg attgaccagt acgggccaat ctcgcggatt cagattgccg agcaaagcca
     6421 gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt atcgaacgcg ggctgatcaa
     6481 agaagttgat cagcaggcct ccaccggggg ccgccgcgct atctccatcg tcaccgaaac
     6541 ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcac gacgccacca tcaccctgtt
     6601 tgatctcagc agcaaagtgc tggcagaaga acattacccg ctgccggaac gtacccagca
     6661 aacgctggaa catgccctgt tgaatgccat tgctcagttt attgatagct accagcgcaa
     6721 actgcgcgag ctgatcgcga tttcggtgat cctgccaggg cttgttgacc cggacagcgg
     6781 caaaattcat tacatgccgc atattcaggt agaaaactgg gggctggtag aagcactgga
     6841 agaacgtttt aaagtgacct gtttcgttgg tcacgatatc cgtagtctgg cgctggcgga
     6901 gcactacttc ggtgcaagtc aggattgcga agactccatt ctggtgcgtg tccatcgcgg
     6961 aaccggggcc gggattatct ctaacgggcg catttttatt ggccgcaacg gcaacgtcgg
     7021 tgaaattggc catattcagg tcgaaccgct gggtgaacgc tgccactgcg gcaactttgg
     7081 ctgcctggaa actatcgctg ccaacgccgc tattgaacaa cgggtgttga atctgttaaa
     7141 gcagggctac cagagccgcg tgccgctgga cgactgcacc atcaaaacta tctgcaaagc
     7201 cgcgaacaaa ggcgatagcc tggcctcgga agtgattgag tatgtcggtc gtcatctggg
     7261 caaaaccatc gccattgcta tcaacttatt taatccgcaa aaaattgtta ttgccggtga
     7321 aatcaccgaa gccgataaag tgctgctccc tgctattgaa agctgcatta atacccaggc
     7381 gctgaaggcg tttcgcacta atctgccggt ggtacgttct gagctggatc accgctcggc
     7441 aatcggcgct tttgcgctgg taaaacgcgc catgctcaac ggtattttgc tccagcattt
     7501 gctggaaaat taatgtgctt ttatagtggc gcttattgtt gtcaatattc tgggtagtcc
     7561 atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc tgatgcacga taacgtcgcc
     7621 gtaccgggtg cagcggaatt tttgcacggg attatggata aaggcctgcc gctggtgttg
     7681 ctgaccaact atccttcgca gaccgggcaa gatctggcga accgctttgc cactgccggt
     7741 gtcgatgtgc cggatagcgt gttttatacc tctgcgatgg cgactgccga tttcctgcgt
     7801 cgccaggaag gcaagaaagc gtatgtggtg ggcgaaggcg cactgattca tgaactgtac
     7861 aaagccggtt tcactattac cgatgtgaac cctgattttg tgattgttgg cgaaacgcgt
     7921 tcctacaact gggacatgat gcataaagcg gcctatttcg tcgctaacgg tgcacgcttt
     7981 atcgccacca acccggacac ccacgggcgc ggtttttatc ccgcttgtgg cgcgttgtgt
     8041 gcagggattg agaaaatctc cgggcgcaaa ccgttctatg ttggtaagcc cagcccgtgg
     8101 atcatccgcg cggcattaaa caaaatgcag gcgcattcgg aagaaacggt gattgtcggc
     8161 gataacttgc gcaccgacat tctggcgggc ttccaggcgg gtctggagac gattctggtg
     8221 ctttctggtg tttcatcgct cgacgatatc gacagtatgc cgttccgccc aagctggatt
     8281 tacccgtcgg tcgctgaaat cgacgttatc tgaatagcaa ccacgtctca gggcgtggtt
     8341 tcttattttt acccatcatt aaaatcatcc cattcaataa aactcgccaa aaattatcga
     8401 tccatcaata aatacatcac cacaccctcc cctttttcac aaatcagcaa tcaccattac
     8461 gttttttatt tttgcactgc caaatcgctt gcgcacggtg ctgctttgcg gcattttttt
     8521 aaacaagcaa acacaacaag caacaaatac caggttaacg gagaaggtta tgtgttcaat
     8581 ttttggcgta ttcgatatca aaacagacgc agttgagctg cgtaagaaag cactcgagct
     8641 gtcacgcctg atgcgtcatc gtggcccgga ctggtccggt atttatgcca gcgataacgc
     8701 cattctcgcc cacgaacgtc tgtcaattgt tgacgttaac gcgggggcgc aacctctcta
     8761 caaccaacaa aaaactcatg tgctggcggt aaacggtgaa atctacaacc accaggcact
     8821 gcgcgccgaa tatggcgatc gttatcagtt ccagaccgga tctgactgcg aagtgatcct
     8881 cgcgctgtat caggaaaaag ggccggaatt tctcgacgac ttgcagggca tgtttgcctt
     8941 tgccctgtac gacagcgaaa aagatgcata tctgattggt cgcgaccatc tggggatcat
     9001 cccactgtat atgggctatg acgagcacgg tcagctgtat gtggcctcag aaatgaaagc
     9061 cctggtgcca gtttgccgca cgattaaaga gttcccggcg gggagctatt tgtggagtca
     9121 ggacggcgaa atccgttctt attatcatcg cgactggttc gactttgatg cagtgaaaga
     9181 taacgtgacc gacaaaaacg agctgcgtca ggcactggaa gattccgtta aaagccatct
     9241 gatgtctgac gttccttacg gtgtgctgct ctcgggtggt ctggattcct caattatttc
     9301 cgcgatcacc aagaaatacg ccgcccgtcg cgtggaagat caggaacgct ctgaagcctg
     9361 gtggccgcaa ttacactcct ttgctgtagg tctgccgggt tcaccggatc tgaaagcagc
     9421 ccaggaagtg gcaaaccatc tgggcacggt gcatcacgaa attcacttca ctgtacagga
     9481 aggtctggat gccatccgcg acgtgattta ccacatcgaa acctatgatg tgacaactat
     9541 tcgcgcttca acaccgatgt atttaatgtc gcgtaagatc aaggcaatgg gcattaaaat
     9601 ggtgctgtcc ggtgaaggtt ctgatgaagt gtttggcggt tatctttact tccacaaagc
     9661 accgaatgcc aaagaactgc atgaagagac ggtgcgtaaa ctgctggccc tgcatatgta
     9721 tgactgcgcg cgcgccaaca aagcgatgtc agcctggggc gtggaagcac gcgttccgtt
     9781 cctcgacaaa aaattccttg atgtggcgat gcgcattaac ccgcaggata aaatgtgcgg
     9841 taacggcaaa atggaaaaac acatcctgcg tgaatgtttt gagtcatacc tgcccgcaag
     9901 cgtggcctgg cggcagaaag agcagttctc cgatggcgtc ggttacagtt ggatcgacac
     9961 cctgaaagaa gtggcggcgc agcaggtttc tgatcagcaa ctggaaactg cccgcttccg
    10021 cttcccgtac aacacgccaa cctcaaaaga agcgtatctg taccgggaga tctttgaaga
    10081 actgttcccg cttccgagcg ccgctgagtg cgtgcctggc ggtccttccg tcgcgtgttc
    10141 ttccgctaaa gcgattgagt gggatgaggc gttcaagaaa atggacgatc cgtctggtcg
    10201 tgcggttggc gttcaccagt cggcgtataa gtaagaaatg cgaaacagcc ccggtgaaat
    10261 ccggggctgt ttcagttatt gaggtgcttg ttaattctga tatgcagtaa ggaaatgttc
    10321 cgggtttgcc acatcactgt ttgacaactc atcactgtag gccagtattt catagattaa
    10381 tttcccacca ctattgttga attccgaatt tcatcctgta cgacaaaaga atggctgtag
    10441 ggtggggaaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10501 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10561 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa agatggctac
    10621 gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt cgaatcccgt
    10681 cgtagccacc atcttttttg cgggagtggc gaaattggta gacgcaccag atttaggttc
    10741 tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc accattcacc agaaagcgtt
    10801 gtacggatgg ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt
    10861 tcgaatccag gtaccccagc catcgaagaa acaat
//
LOCUS       DAZFKM010000158        10560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFKM010000158 DAZFKM010000000
VERSION     DAZFKM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            192..767
                     /gene="ssuE"
                     /locus_tag="ACHKAY_004372"
     CDS             192..767
                     /gene="ssuE"
                     /locus_tag="ACHKAY_004372"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO5702936.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            760..1719
                     /gene="ssuA"
                     /locus_tag="ACHKAY_004373"
     CDS             760..1719
                     /gene="ssuA"
                     /locus_tag="ACHKAY_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO5702937.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1716..2861
                     /gene="ssuD"
                     /locus_tag="ACHKAY_004374"
     CDS             1716..2861
                     /gene="ssuD"
                     /locus_tag="ACHKAY_004374"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO5702938.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2872..3663
                     /gene="ssuC"
                     /locus_tag="ACHKAY_004375"
     CDS             2872..3663
                     /gene="ssuC"
                     /locus_tag="ACHKAY_004375"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO5702939.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3660..4427
                     /gene="ssuB"
                     /locus_tag="ACHKAY_004376"
     CDS             3660..4427
                     /gene="ssuB"
                     /locus_tag="ACHKAY_004376"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO5702940.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4634..7246)
                     /gene="pepN"
                     /locus_tag="ACHKAY_004377"
     CDS             complement(4634..7246)
                     /gene="pepN"
                     /locus_tag="ACHKAY_004377"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO5702941.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7512..8714
                     /gene="pncB"
                     /locus_tag="ACHKAY_004378"
     CDS             7512..8714
                     /gene="pncB"
                     /locus_tag="ACHKAY_004378"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO5702942.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8883..10283
                     /gene="asnS"
                     /locus_tag="ACHKAY_004379"
     CDS             8883..10283
                     /gene="asnS"
                     /locus_tag="ACHKAY_004379"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO5702943.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2402 a         2715 c         2818 g         2625 t
ORIGIN      
        1 taattcattg attcaacatc taatgactat ccaacaaata aatatgcgtg atatataaag
       61 ccctttcttt agtttatttt cagaaaaaga tacacaaaat ttatatttgg cattttcttg
      121 tctctccgac agactgaccg aattgttatc aatgttaaca aaaaaagaac aattggttat
      181 aaggagagag tatgcgtgtc atcaccctgg cgggtagtcc tcgctttcct tctcgctcca
      241 gctccttgct ggaatatgcg cgggaaaaac taaattgcct ggatgtagag gtttatcact
      301 ggaatctgca aaacttcgcc ccggaagatc tgctttacgc tcgtttcgat agtccggcac
      361 tcaagacctt caccgaacag ctgcaacagg ctgatggact gatagtcgct acacctgtgt
      421 ataaagccgc ctattccggc gcgctgaaaa ccctgctcga cctgctgcca gagcgcgctt
      481 tgcaaggcaa agtggtgcta ccgctggcga cgggcggtac ggtggctcat ctgctggcgg
      541 tcgattatgc ccttaagcca gttttaagtg cgatgaaagc ccaggagatc ctgcacggcg
      601 tgtttgccga tgactcacaa gttattgatt accatcacaa accccagttc acgccaaatc
      661 tgcaaacccg tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg
      721 ttcaggttcc tgaccttctg tctctgcgag gtaatgtcca tgcgtaacat cattaaactg
      781 gcgctggcgg gattgcttag cgtctctact tttgcggttg ctgcagaatc ctcgcctgaa
      841 gcgttacgta taggctatca gaaaggcagt attggtatgg tattggcaaa aagccaccag
      901 ttactggaaa aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg
      961 caaatgttgg aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg
     1021 ccaatctttg cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag
     1081 cccaaagccg aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt
     1141 aaaggtcaca aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca
     1201 ctgcgtcagg ccggacttaa gtttaccgat atccaaccca cttacctgac gcccgctgat
     1261 gcccgcgccg cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac
     1321 tctgctgcat tattacaggg cgccgtgcgg gtgctgaaag acggcaccga tctcaatcaa
     1381 actggatcgt tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag
     1441 ggcgtactcg caacctttag tgaggccgat gcgttaaccc gcagccagcg cgagcaaagc
     1501 atcgctttac tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat
     1561 catcgccctc ctaccaccat caaaccggtt aacgccaagg ttgccgcctt acagcagcaa
     1621 acggcagatc tgttttatga aaatcgtctg gtgccgaaaa aagtcgatat tcgccagcgc
     1681 atctggcagc ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt
     1741 taccgaccca tggtgacggg cattatcttg gaacggaaga aggttcacgc ccggttgatc
     1801 acggctatct gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga
     1861 tcccaacggg gcgatcgtgt gaagatgcgt ggctggtggc ggcgtcgatg atcccggtga
     1921 cgcagcggct gaagtttctt gtcgccctgc gccccagcgt aacctcacct accgttgccg
     1981 ctcgccaggc cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca
     2041 caggcagcga tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct
     2101 acgaagcctc ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca
     2161 atttcaacgg taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac
     2221 agccttatcc tccgctttac tttggcggat cgtcagacgt tgcccaggag ctggcggcag
     2281 aacaagttga tctctacctc acctggggcg aaccgccaga actagtaaaa gagaagatcg
     2341 aacaagtgcg ggcgaaagct gccgcgcatg gacgcaaaat tcgtttcggt attcgtctgc
     2401 atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc
     2461 atcttgatga tgaaactatc gccaaagcac aggccgcatt cgcccggacg gattccgtag
     2521 gacaacagcg aatggcggcg ttacataacg gcaagcgcga caatctggag atcagcccca
     2581 atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg
     2641 gtcctacggt cgctgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc
     2701 tttcgggcta tccgcatctg gaagaagcgt atcgggtcgg cgagttgctg ttcccacatc
     2761 tggatgtcgc cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg
     2821 tggcgaatga ttttatcccc cgtaaagtcg cgcaaagcta aggaagaaat aatggcaacg
     2881 ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt taccggtggg catcgtggcg
     2941 gtgtggcaac tggcctcctc agttggctgg ctgtcgacgc gtattttgcc ctcaccggaa
     3001 ggggtggtga cggcgttctg gacgctctcc gccagcggcg aactgtggca gcatctggcg
     3061 atcagttcct ggcgggcgct gattggcttt tcaattggcg gatcgctggg gttgattttg
     3121 ggactgatta gcgggctatc tcgctgggga gaacggctgc tggatacctc aattcagatg
     3181 ttgcgcaacg taccgcatct ggcgctgatc ccgctggtga ttttgtggtt tggcattgat
     3241 gaatccgcaa aaatctttct ggtggcgctc ggcacgctat tccccattta tatcaacacc
     3301 tggcatggca tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag ctatggatta
     3361 tccggcatac cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc aattatggtc
     3421 ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga aaccatttct
     3481 gccaattcag gcattggtta tctggcgatg aatgcgcggg agtttttgca aacggacgtg
     3541 gtggtagtcg ctattattct ttacgccctg ctcggcaaac ttgctgacgt cagcgcgcag
     3601 ctgttagagc gcctctggtt gcgctggaac ccggcttatc atttgaagga ggccacggta
     3661 tgaatactgc tcgtctgaac cagggcacac cattgttgct caatgcagta agcaaacatt
     3721 acgcggaaaa tatcgtcctg aaccaactgg atttacatat tccggcaggt cagtttgtgg
     3781 cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg gcaggtctgg
     3841 aaacgccaac cgcaggcgat gtgttagcgg gcaccacacc gctggctgag attcaagaag
     3901 acacgcgaat gatgtttcag gatgcgcgtc tgctgccgtg gaaatcggtg attgataacg
     3961 ttgggttagg ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg gctgcagtag
     4021 gactggagaa tcgcgccggg gaatggcctg ccgcactttc tggcgggcag aaacagcgtg
     4081 tggcgctggc aagagcgttg attcatcgac cgggtttatt gttgcttgat gaaccgctcg
     4141 gggcgctgga cgccttaacg cgactcgaga tgcaggattt gattgtgtca ctttggcagc
     4201 agcacggctt taccgtactg ttggtgacgc atgatgtgag cgaagcggta gcaatggctg
     4261 accgggtgtt gttaattgaa gagggaaaaa ttggtctgga tttgacagtg gatatccccc
     4321 gtccgcgtcg attagggtcg gtgcgattag cggagctgga agcggaagta ttgcaacggg
     4381 taatgcggcg tgggcattct gaacaactga ttcggcgtca tggttaatat gccggatgtg
     4441 gcgcacaaaa ccagcatttt caacggacta gtaggcctga taagacacgt caagcatcgc
     4501 atcaggcagt tgggcacggc tgccggatgc ggcgtgaacg ccttatccag cctacaaaac
     4561 cagcaatttc aacggagttg taggcctgat aagacgcgtc aagcgtcgca tcaggcattt
     4621 tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc gtacagatcg ccagagagat
     4681 tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt ttggcatcat
     4741 aacgtttcag gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg ttacggctgt
     4801 tgaggtcggt aagcatttcc accaggaact ggtaaccgct gccatcttcg gcatggaacg
     4861 ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatacggttc gggttgctca
     4921 tggtaaatga gcgatgctgc aacagaccac gcaccgtctc cagcacattc gccgccgggc
     4981 tggtggcttg caggataaac catttatcca tcaccagacc gtcctgatgc cacttatcgt
     5041 cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc gcagaaagcg
     5101 ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc agcacgtcag
     5161 ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga gtgcgttttg
     5221 caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa atagccagca
     5281 gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact tcggcaatag
     5341 caatcgggtc gatgatatcg aacagttcag ccatttcatt gacagaaggc agcgtcagga
     5401 tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg aaagcgtcag
     5461 ccacatgtac cggcagagac agcggctgcc cttgctgatg acgggcgacg ttcagcttga
     5521 tgtaggttgc cagcaaactt tgcgccgcat cccaacggga gaaatcatta cgcgcatgac
     5581 gcatcaggaa ggtcagttgc tgatcgctcc acttatattc cagtttcact ggcgcagaga
     5641 attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca aaggtctgtt
     5701 ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc aacgggatca
     5761 ctttgccttc gttgtcatac aattcgatgg caaatggaat atgcagcggc tgtttttctg
     5821 cctgatccgg cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc tcggtttccg
     5881 gattgtagtc gtctttgacg gtcacaatcg gcgtgccgga ctggctgtac caacggcgga
     5941 aatgggagag atcgacattc gacgcatctt ccatcgcctg cacaaagtcg tcgcaggttg
     6001 ccgcgctgcc atcatggcgc tcgaaataga gttgcatccc tttctggaag ttttcttcgc
     6061 ccagcagggt gtggatcatg cgaatcactt ccgcgccctt ctcgtaaacg gtcagggtgt
     6121 agaagttgtt catctcaatg accatatccg ggcggatcgg gtgcgccatc gggctggcgt
     6181 cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt gatacggttt accgcgcggg
     6241 aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct ttcaggctga
     6301 gctggaacca gtcgcggcag gtcactcggt taccggtcca gttatggaaa tattcatggc
     6361 cgataacgcg ttcaatatcg agataatctt tgtcggtggc ggtgtcggtg cgggccaaca
     6421 catatttgga gttaaagata ttcagaccct tattctccat tgcgcccata ttgaagaaat
     6481 ccaccgcgac gatcatatag atgtcgaggt catactccag gccaaagcgt tcttcatccc
     6541 atttcatgga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg ttgccgcgat
     6601 cgacgtacag ctccagtgct acttcgcgac cagaacgcgt ggtaaaggtg tcgcgcagta
     6661 catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac gggtcctgcc
     6721 actgtaccca atggcgtccg ttttccagtt caccttgcgc aacgcggtta ccgttggaaa
     6781 gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc gccagcacgt
     6841 ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa
     6901 gcgcatcgcc tgactgataa agcccttcca gcgccgtatt cgccgccggg cttatttcat
     6961 taatgatctt aagcgtaaaa cgctccggca aattactgat gaccagtgcg ccctcttctt
     7021 ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagtttg aggtcttcgc
     7081 cgttgagacg aagcggagca tctgatgcac catgacggac agcctggctg accgcggtaa
     7141 cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc tggtaatccg
     7201 gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat aacctttagc
     7261 atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat
     7321 cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata caccgcgtaa
     7381 tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt gcgaaacaag
     7441 gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta cgaggacgct
     7501 actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg gatacagatg
     7561 cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg
     7621 cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct attcgtgaac
     7681 agattcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg ctctctgccc
     7741 tgcctttctt taaggccgac tatcttaact ggttacgcga gttccgcttt aacccggaac
     7801 aagtcaccgt atccaacgat aatggcaagc tggatattcg tttaagcggc ccgtggcgtg
     7861 aagtcatcct ctgggaagtt cctttgctgg cggttatcag tgaaatggta catcgctatc
     7921 gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa ttagtcgact
     7981 tctcggcgtt aaccgccggc cttgatatgt cgcgcttcca tcttatggat tttggcaccc
     8041 gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa caggaatcct
     8101 ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa
     8161 cacaggcaca cgaatggttc caggcacatc agcaaatcag cccggatcta gccaacagcc
     8221 agcgagctgc acttgctgcc tggctggaag aatatcccga ccaacttggc attgcattaa
     8281 ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc gccagtcgct
     8341 atcagggcct gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa gccattgcac
     8401 attatgaaaa gctgggaatt gatccacaga gtaaaactct ggttttctct gacaatctgg
     8461 atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg
     8521 gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat attgtgatta
     8581 agttggttga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct ggcaaaacta
     8641 tctgccacga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta
     8701 aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc tttaattccg
     8761 aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag gtaacatagg
     8821 tatcccccca tttacgggtg gacaagtgtt tattttttcc gactattaac agagagaata
     8881 ttatgagcgt tgtgcctgta gccgacgtac tccagggccg cgtagccgtt gacagcgaag
     8941 tcaccgtacg cggatgggta cgtacccgcc gagattcaaa agctggcatc tccttcctcg
     9001 ccgtttatga cggttcctgc tttgatcctg tacaggctgt catcaataat tctctgccca
     9061 attacaatga agacgtcctg cgtctgacca ccggctgctc ggtcattgtg acgggtaaag
     9121 tcgtggcgtc gccgggccag gggcaacaat ttgaaattca ggccagcaag gttgaagttg
     9181 ctggttgggt tgaagatcca gacacttacc cgatggcggc aaaacgccac agcattgagt
     9241 atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc gtcgcgcgcg
     9301 ttcgtcatac gctggcgcag gcgctgcatc gcttctttaa cgagcaggga ttcttctggg
     9361 tttcaacgcc actgattacc gcatctgata ccgaaggtgc aggcgaaatg ttccgcgttt
     9421 ctacgctgga tctggaaaac ctgccgcgta acgatcaggg caaagtggat ttcgacaaag
     9481 acttctttgg taaagagtct ttcctgaccg tatctggcca gttgaacggc gaaacctacg
     9541 cttgcgcatt atccaaaatt tataccttcg gcccgacttt ccgtgctgaa aactccaaca
     9601 ccagccgtca cctggcggaa ttctggatgc tggagccgga agtggcgttt gctaacctga
     9661 acgatatcgc gggtctggct gaagccatgc tgaaatatgt cttcaaagcg gttctcgaag
     9721 aacgcgcaga cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc gtttcacgtc
     9781 tggaacgctt cattgaggcc gatttcgcgc aggtggatta caccgacgca gtgaccattc
     9841 tcgaaaactg cggcaggaag tttgaaaacc cggtttactg gggcgtcgat ctctcttctg
     9901 agcatgagcg ttatctggcg gaagaacact ttaaagcacc ggtagtggtt aaaaactatc
     9961 cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga cggtaaaacc gttgcggcta
    10021 tggacgttct ggctccgggc atcggtgaga tcattggtgg ctcccagcgt gaagaacgtc
    10081 tggacgttct ggacgagcgt atgctggaaa tgggcctgaa taaagaagat tactggtggt
    10141 atcgcgatct gcgtcgctac ggtactgttc cgcattcagg tttcggtctt ggttttgaac
    10201 gcctgattgc ttacgtaact ggcgtgcaaa acgtgcgtga tgtgattccg ttcccacgta
    10261 ctccgcgcaa cgccagcttc tgattttctt cctcaaagag ccagcgcctg ctggctcttt
    10321 tttatccttt ctgtcaagtt atctgtttgt taagtcaagc aatctatttg caaccccgcc
    10381 ataacgatca tcctgttacg gaatattaca ttgcaacatt tacgtacaaa aaataacccg
    10441 cattcttatt gcggattagt tttttcttag ctgatagcac aattttcata ctattttttg
    10501 gcattctgga tgtctgaaag aggattttgt gccagttcga taaagtttcc atcagaaaca
//
LOCUS       DAZFKM010000159        10497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFKM010000159 DAZFKM010000000
VERSION     DAZFKM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            564..2132
                     /gene="cydA"
                     /locus_tag="ACHKAY_004380"
     CDS             564..2132
                     /gene="cydA"
                     /locus_tag="ACHKAY_004380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO5702944.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2148..3287
                     /gene="cydB"
                     /locus_tag="ACHKAY_004381"
     CDS             2148..3287
                     /gene="cydB"
                     /locus_tag="ACHKAY_004381"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO5702945.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3302..3415
                     /gene="cydX"
                     /locus_tag="ACHKAY_004382"
     CDS             3302..3415
                     /gene="cydX"
                     /locus_tag="ACHKAY_004382"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO5702946.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3415..3708
                     /gene="ybgE"
                     /locus_tag="ACHKAY_004383"
     CDS             3415..3708
                     /gene="ybgE"
                     /locus_tag="ACHKAY_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO5702947.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3858..4262
                     /gene="ybgC"
                     /locus_tag="ACHKAY_004384"
     CDS             3858..4262
                     /gene="ybgC"
                     /locus_tag="ACHKAY_004384"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO5702948.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4259..4951
                     /gene="tolQ"
                     /locus_tag="ACHKAY_004385"
     CDS             4259..4951
                     /gene="tolQ"
                     /locus_tag="ACHKAY_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO5702949.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4955..5383
                     /gene="tolR"
                     /locus_tag="ACHKAY_004386"
     CDS             4955..5383
                     /gene="tolR"
                     /locus_tag="ACHKAY_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO5702950.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5448..6713
                     /gene="tolA"
                     /locus_tag="ACHKAY_004387"
     CDS             5448..6713
                     /gene="tolA"
                     /locus_tag="ACHKAY_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO5702951.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6846..8138
                     /gene="tolB"
                     /locus_tag="ACHKAY_004388"
     CDS             6846..8138
                     /gene="tolB"
                     /locus_tag="ACHKAY_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO5702952.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8173..8694
                     /gene="pal"
                     /locus_tag="ACHKAY_004389"
     CDS             8173..8694
                     /gene="pal"
                     /locus_tag="ACHKAY_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO5702953.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8704..9495
                     /gene="cpoB"
                     /locus_tag="ACHKAY_004390"
     CDS             8704..9495
                     /gene="cpoB"
                     /locus_tag="ACHKAY_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO5702954.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9660..9735
                     /locus_tag="ACHKAY_004391"
     tRNA            9660..9735
                     /locus_tag="ACHKAY_004391"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9693..9695,aa:Lys,seq:ttt)
     gene            9871..9946
                     /locus_tag="ACHKAY_004392"
     tRNA            9871..9946
                     /locus_tag="ACHKAY_004392"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9904..9906,aa:Val,seq:tac)
     gene            9949..10024
                     /locus_tag="ACHKAY_004393"
     tRNA            9949..10024
                     /locus_tag="ACHKAY_004393"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9982..9984,aa:Lys,seq:ttt)
     gene            10174..10249
                     /locus_tag="ACHKAY_004394"
     tRNA            10174..10249
                     /locus_tag="ACHKAY_004394"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10207..10209,aa:Val,seq:tac)
     gene            10253..10328
                     /locus_tag="ACHKAY_004395"
     tRNA            10253..10328
                     /locus_tag="ACHKAY_004395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10286..10288,aa:Lys,seq:ttt)
BASE COUNT         2587 a         2490 c         2826 g         2594 t
ORIGIN      
        1 ggtggattta tgcctttatt agtaatcctg aaactctgcg tcgtattagc cagtgaccaa
       61 aaaaagaatt aaggtcaacc gtgctgtttt tgcttcgtct ctttttatct ttaattgcca
      121 accgaaacta atttcagcct tataactcac acattttaaa cataaatgtc actaaagtta
      181 ccttattgaa acatgattaa cataatttgt aggaattgat atttatcaat gtataagtct
      241 tggaaatggg catcaaaaag agataaattg ttctcgatca aattggctga aaggcggtaa
      301 tttagctata aattgatcac cgtcgaaaaa tgcaaatttg cttcaacaaa aacctgttta
      361 ttgtaaggat tttgcggcgt aatatatacg tgggatcaat ttgagttttt attaacatgt
      421 ttgcaacctt tctttacgcc gtttttgtgt gcattcacat ggtatgatga aagtgttcaa
      481 acaaatttct attggggcat gcgtgtgacc ctttctaacg gggttcactc tcggagtctt
      541 catgcgatga gcaaggagtc atgatgttag atatagtcga actgtcgcgc ttacagtttg
      601 ccttgaccgc gatgtaccac ttcctttttg tgccactgac gctcggtatg gcgttcctgc
      661 tggccattat ggaaacggtc tacgtcctct ccggcaaaca gatttataaa gatatgacca
      721 agttctgggg caagttgttt ggtatcaact tcgctctggg tgtggctacc ggtctgacca
      781 tggagttcca gttcgggact aactggtctt actattccca ctatgtaggg gatatcttcg
      841 gtgcgccgct ggcaatcgaa ggtctgatgg ccttcttcct cgaatccacc tttgtaggtc
      901 tgttcttctt cggttgggat cgtctgggta aagttcagca tatgtgtgtc acctggctgg
      961 tggcgctcgg ttcaaacctg tccgcactgt ggattctggt tgcgaacggc tggatgcaaa
     1021 acccaatcgc gtccgatttc aactttgaaa ctatgcgtat ggagatggtg agcttctccg
     1081 agctggtgct taacccggtt gctcaggtga aattcgttca cactgtagcg tctggttatg
     1141 tgactggcgc gatgttcatc ctcggtatca gcgcatggta tatgctgaaa ggtcgtgact
     1201 tcgccttcgc taaacgctcc tttgctatcg ctgccagctt cggtatggct gctgttctgt
     1261 ctgttattgt tctgggtgat gaatccggct acgaaatggg cgacgtgcag aaaaccaaac
     1321 tggctgctat tgaagccgag tgggaaacgc aacctgcgcc tgctgccttt actctgttcg
     1381 gcattcctga tcaggaagag gagacgaaca aatttgcgat tcagatccct tacgcactgg
     1441 gcatcattgc aacgcgttcc gtggataccc cggttatcgg cctgaaagag ctgatggtgc
     1501 agcatgaaga acgcattcgt aacgggatga aggcgtactc tctgctcgaa caactgcgtt
     1561 ctggttctac cgaccaggcg gttcgtgacc agttcaatag catgaagaaa gacctcggtt
     1621 acggtctgct gctgaaacgc tatacgccaa acgtggctga tgcgactgaa gcgcagattc
     1681 aacaggcaac caaagactcc atcccgcgtg tagcgccgct gtactttgcg ttccgtatca
     1741 tggtggcgtg tggcttcctg cttctggcaa tcatcgcgct ctctttctgg agtgtcatcc
     1801 gcaaccgcat tggcgagaaa aaatggcttc tgcgcgccgc gctgtacggt attccgctgc
     1861 cgtggattgc tgtagaagcg ggctggttcg tggctgaata tggccgccaa ccgtgggcta
     1921 tcggtgaagt gctgccgaca gctgtggcga actcgtcact gaccgcaggc gatctcatct
     1981 tctcaatggt gctgatttgc ggcctgtata ccctgttcct ggtggcagaa ttgttcttaa
     2041 tgttcaaatt tgcacgcctc ggcccaagca gcctgaaaac cggtcgctat cactttgagc
     2101 agtcttccac gactactcag ccggcacgct aagacaggag tcgtcaaatg atcgattatg
     2161 aagtattgcg ttttatctgg tggctgctgg ttggcgttct gctgattggt tttgcagtca
     2221 ctgacggttt cgacatgggg gtgggcatgc tcacccgttt cctcggtcgt aacgacaccg
     2281 agcgtcgaat tatgattaac tccattgcac cacactggga cggtaaccag gtttggctga
     2341 tcaccgcggg cggcgcactc tttgctgcct ggccgatggt ctatgccgct gcgttctccg
     2401 gcttctatgt ggcgatgatc ctcgtgctgg cgtctttgtt cttccgtccg gtcggttttg
     2461 actaccgctc caagattgaa gaaacccgct ggcgtaacat gtgggactgg ggcatcttca
     2521 ttggtagctt cgttccgccg ctggtaattg gtgtagcgtt cggtaacctg ttgcagggcg
     2581 taccgttcaa cgttgatgaa tatctgcgtc tgtactacac cggtaacttc ttccagttgc
     2641 ttaacccgtt cggcctgctg gcaggcgtgg tgagcgtagg gatgatcatt actcagggcg
     2701 caacctatct gcaaatgcgt accgtgggcg aactgcacct gcgtacccgt gcaacggctc
     2761 aggtggctgc gctggtgaca ctggtctgtt tcgcactggc tggcgtatgg gtgatgtacg
     2821 gtatcgatgg ttatgtcgtg aaatcgacaa tggaccatta cgcagcctct aacccactga
     2881 ataaagaagt ggttcgtgaa gctggcgcat ggctggttaa cttcaacaac acgccaattc
     2941 tgtgggctat tccggcactg ggtgtggttc tgccgctgct gaccatcctg actgcacgta
     3001 tggataaagc cgcgtgggcg tttgtgttct cctccctgac gctggcctgc atcatcctga
     3061 cagccggtat cgcaatgttc ccgtttgtga tgccgtccag caccatgatg aacgcaagtc
     3121 tgacaatgtg ggatgcaact tccagccagc tgacgcttaa cgtcatgacc tgggttgcgg
     3181 tggttctggt accgatcatt ctgctctaca ccgcctggtg ttactggaaa atgttcggtc
     3241 gtatcaccaa agaagatatt gaacgtaaca cccactctct gtactaagta aggagctaaa
     3301 aatgtggtat ttcgcatgga ttctgggaac gcttcttgcc tgttcgtttg gggtaatcac
     3361 cgcgctggcg cttgaacacg tcgaatcagg caaagccggt caagaagaca tctgatgagt
     3421 aagattatcg cgactttgta tgcggtaatg gacaagcgcc ccctgcgggc gctttccttc
     3481 gtgatggcgc ttctgttagc aggatgtatg ttttgggacc catctcgttt tgccgcgaag
     3541 accagtgaac tggaaatctg gcatggttta ttgctgatgt gggccgtctg tgctggtgtg
     3601 attcacggcg tgggctttcg tccgcagaag gttctttggc aagggatttt ttgcccattg
     3661 cttgccgata ttgttctcat tgtcgggctg attttcttct tcttttaaat cagaattctc
     3721 tttaaaaaat tatgggccgc tccaggccca taaattttta cgctccctta acttgccctc
     3781 attcccaaac ctcaatcgcg cgcgtatagt agcagcgttt aaaagctcta acttttgttg
     3841 cattaccggg atgtaaagtg aatacaacgc tgtttcgatg gccggttcgc gtctactatg
     3901 aagataccga tgccggtggt gtggtgtacc acgccagtta cgtcgctttt tatgaaagag
     3961 cacgcacaga gatgctgcgt catcatcact tcagtcagca ggcgctgatg gctgaacgcg
     4021 ttgcctttgt ggtacgtaaa atgacggtgg aatattacgc acctgcgcgg ctcgacgata
     4081 tgctcgaaat acagactgaa ataacatcaa tgcgtggcac ctctttggtt ttcacgcaac
     4141 gtattgtcaa cgccgagaat actttgctga atgaagcaga ggttctggtt gtttgcgttg
     4201 acccactcaa aatgaagcct cgtgcgcttc ccaagtctat tgtcgcggag tttaagcagt
     4261 gactgacatg aatatccttg atttgttcct gaaggctagc cttctggtta aacttatcat
     4321 gttgattttg attggttttt caatcgcatc ctgggccatt attatccagc ggacccgtat
     4381 tcttaacgca gcggcgcgcg aagccgaagc gtttgaagat aaattctggt ctggaatcga
     4441 actctctcgc ctctatcaag agagtcaggg gaaacgggat aatctgactg gttcggaaca
     4501 aatattttac agcgggttca aagagtttgt gcgcctgcat cgtgccaata gccatgcgcc
     4561 ggaagccgta gtggaagggg cgtcgcgtgc tatgcgtatc tccatgaacc gtgaacttga
     4621 aaatctggaa acgcacattc cgttcctcgg tacggttggc tccatcagcc cgtatattgg
     4681 tctgtttggt acggtctggg ggatcatgca cgcctttatc gccctcgggg cggtaaaaca
     4741 agcaacactg caaatggttg cgcccggtat cgcagaagcg ttgattgcga ctgcaattgg
     4801 tctgtttgcc gctatcccgg cagttatggc ctacaaccgc ctcaaccagc gcgtaaacaa
     4861 actggaactg aattacgaca actttatgga agagtttacc gcgattctgc accgccaggc
     4921 gtttaccgtt agcgagagca ataaggggta agtcatggcc agagcgcgtg gacgaggtcg
     4981 tcgcgaactt aagtccgaaa tcaacattgt accgttgctg gacgtactgc tggtgctgtt
     5041 gctgatcttc atggcgacag cgcccatcat cacccagagc gtggaggtcg atctgccaga
     5101 cgctactgaa tcacaggcgg tgagcagtaa cgataatccg ccagtgattg ttgaagtgtc
     5161 tggtattggt cagtacaccg tggtggttga gaaagatcgc ctggagcgtt taccaccaga
     5221 gcaggtggtg gcggaagtgt ccagccgttt caaggccaac ccgaaaacgg tctttctgat
     5281 cggtggcgca aaagatgtgc cttacgatga aataattaaa gcactgaact tgttacatag
     5341 tgcgggtgtg aaatcggttg gtttaatgac gcagcctatc taaacatctg cgtttccctt
     5401 gcttgaaaga gagcgggtaa caggcgaaca gtttttggaa accgagagtg tcaaaggcaa
     5461 ccgaacaaaa cgacaagctc aagcgggcga taattatttc agcagtgctg catgtcatct
     5521 tatttgcggc gctgatctgg agttcgttcg atgagaatat agaagcttca gccggaggcg
     5581 gcggtggttc gtccatcgac gctgtcatgg ttgattcagg tgcggtagtt gagcagtaca
     5641 aacgcatgca aagccaggaa tcaagcgcga agcgttctga tgaacagcgc aagatgaagg
     5701 aacagcaggc tgctgaagaa ctgcgtgaga aacaagcggc tgaacaggaa cgcctgaagc
     5761 aacttgagaa agagcggtta gcggctcagg agcagaaaaa gcaggctgaa gaagccgcaa
     5821 aacaggccga gttaaagcag aagcaagctg aagaggcggc agcgaaagcg gcggcagatg
     5881 ctaaagcgaa ggccgaagca gatgctaaag ctgcggaaga agcagcgaag aaagcggctg
     5941 cagacgcaaa gaaaaaagca gaagcagaag ccgccaaagc cgcagccgaa gcgcagaaaa
     6001 aagccgaggc agccgctgcg gcactgaaga agaaagcgga agcggcagaa gcagctgcag
     6061 ctgaagcaag aaagaaagcg gcaactgaag ctgctgaaaa agccaaagca gaagctgaga
     6121 agaaagcggc tgctgaaaag gctgcagctg ataagaaagc ggcagcagag aaagctgcag
     6181 ccgacaaaaa agcagcagaa aaagcggctg ctgaaaaggc agcagctgat aagaaagcag
     6241 cggcagaaaa agccgccgca gacaaaaaag cggcagcggc aaaagctgca gctgaaaaag
     6301 ccgctgcagc aaaagcggcc gcagaggcag atgatatttt cggtgagcta agctctggta
     6361 agaatgcacc gaaaacgggg ggaggggcga aagggaacaa tgcttcgcct gccgggagtg
     6421 gtaatactaa aaacaatggc gcatcagggg ccgatatcaa taactatgcc gggcagatta
     6481 aatctgctat cgaaagtaag ttctatgacg catcgtccta tgcaggcaaa acctgtacgc
     6541 tgcgcataaa actggcaccc gatggtatgt tactggatat caaacctgaa ggtggcgatc
     6601 ccgcactttg tcaggctgcg ttggcagcag ctaaacttgc gaagatcccg aaaccaccaa
     6661 gccaggcagt atatgaagtg ttcaaaaacg cgccattgga cttcaaaccg taatcgcgat
     6721 gttgactgtt cggacggtca acatcaggca ccggttgcca cggggttctg gtagttttgt
     6781 gtattttagt ttgttaacat tctgctaaat tatcgtgggc catcggtcca gataagggag
     6841 atatgatgaa gcaggcatta cgagtagcat ttggttttct catactgtgg gcatcagttc
     6901 tgcatgctga agtccgcatt gtgatcgaca gcggtgtaga ttccggtcgt cctattggtg
     6961 ttgttccttt ccagtgggcg gggcctggtg cggcacctga agatattggc ggcatcgttg
     7021 ctgctgactt gcgtaacagc ggtaaattta atccgttaga tcgcgctcgt ctgccacagc
     7081 agccgggtag tgcgcaggaa gtacaaccag ctgcatggtc cgcactgggc attgacgctg
     7141 tagttgtcgg tcaggtcact ccgaatccgg atggttctta caatgttgct tatcaacttg
     7201 ttgacactgg cggcgcaccg ggtactgtac ttgctcagaa ctcgtacaaa gtgaacaagc
     7261 agtggctgcg ttatgctggt cataccgcca gtgatgaagt gtttgaaaaa ctgaccggca
     7321 ttaaaggtgc gttccgtact cgtattgcct acgttgttca gaccaacggc ggtcagttcc
     7381 cgtatgaact gcgcgtatct gactatgacg gttacaacca gtttgtcgtt caccgttcac
     7441 cgcagccgct gatgtctccg gcgtggtcac cagacggttc taaactggct tatgtgacct
     7501 tcgaaagcgg tcgttccgcg ctggttattc agacgctggc aaatggcgct gtacgtcagg
     7561 tggcttcatt cccgcgtcac aacggtgcac ctgcattctc gccagacggc agcaaactgg
     7621 cattcgcctt gtcgaaaacc ggtagtctga acctgtacgt aatggatttg gcttctggtc
     7681 agatccgcca ggtgactgat ggtcgcagta acaataccga accgacctgg ttcccggata
     7741 gccagaacct ggcatttact tctgaccagg ccggtcgtcc gcaggtttat aaagtgaata
     7801 tcaacggcgg tgcgccacaa cgtattacct gggaaggttc gcagaaccag gatgcggatg
     7861 tcagcagcga cggtaaattt atggtaatgg tcagctccaa tggtgggcag cagcacattg
     7921 ccaaacaaga tctggcaacg ggaggcgtac aagttctgtc gtccacgttc ctggatgaaa
     7981 cgccaagtct ggcacctaac ggcactatgg taatctacag ctcttctcag gggatgggat
     8041 ccgtgctgaa tttggtttct acagatgggc gtttcaaagc gcgtcttccg gcaactgatg
     8101 gacaggtcaa attccctgcc tggtcgccgt atctgtgata ataattaatt gaatagtaaa
     8161 ggaattattg aaatgcaact gaacaaagtg ctgaaagggc tgatgattgc tctgcctgtt
     8221 atggcaattg cggcatgttc ttccaacaag aacgccagca atgacggcag cgaaggcatg
     8281 ctgggtgccg gcactggtat ggatgcgaac ggcggcaacg gcaacatgtc ttccgaagag
     8341 caggctcgtc tgcaaatgca acagctgcag cagaacaaca tcgtttactt cgatctggac
     8401 aagtacgata tccgttctga cttcgctcaa atgctggatg cacatgcaaa cttcctgcgt
     8461 agcaacccgt cttacaaagt caccgtagaa ggtcacgcgg acgaacgtgg tactccggaa
     8521 tacaacatct ccctgggtga acgtcgtgcg aacgccgtta agatgtacct gcagggtaaa
     8581 ggcgtttctg cagaccagat ctccatcgtt tcttacggta aagaaaaacc tgcagtactg
     8641 ggtcatgacg aagcggcata ctccaaaaac cgtcgtgcgg tactggttta ctaagagaat
     8701 tgcatgagca gtaacttcag acatcaacta ttgagtctgt cgttactggt tggtatagcg
     8761 gccccctggg ccgcttttgc tcaggcacca atcagtagtg tcggctcagg ctcggtcgaa
     8821 gaccgcgtca ctcaacttga gcgtatttct aatgctcaca gccagctttt aacccaactc
     8881 cagcaacaac tttctgataa tcaatccgat attgattccc tgcgtggtca gattcaggaa
     8941 aatcagtatc aactgaatca ggtcgtggag cggcaaaagc agatcctgtt gcagatcgac
     9001 agcctcagca gcggtggtgc agcggcgcaa tcaaccagca gcgatcaaag cggtgcggcg
     9061 gcatcaacga cgccgacagc tgatgctggt actgcgaatg ctggcgcgcc ggtgaaaagc
     9121 ggcgatgcaa acacggatta caatgcagct attgcgctgg tgcaggataa atcccgccag
     9181 gatgacgcaa tggtggcatt tcagaatttc atcaaaaatt accctgattc aacttacctg
     9241 ccaaacgcca attattggct gggtcagtta aactacaaca agggtaaaaa agatgatgcg
     9301 gcgtactatt ttgcttcggt agtgaaaaac tatccgaagt caccaaaggc tgctgatgcg
     9361 atgtttaaag tcggcgtcat catgcaggac aaaggtgaca ccgcaaaagc gaaagccgta
     9421 taccagcagg ttatcagtaa ataccctggt actgatggcg ctaaacaggc acaaaaacgt
     9481 ctgaacgcga tgtaatgcat aacacacgac cagaagtcgc attatttctg gtcgtgtcgt
     9541 gcgaatcata agcagttgag tgatctgcat cgaaattttt gttgcgctca agtctgaaat
     9601 cagtaatata tgccgccgtt gccacgggat atcaaacaaa ccgaaagcaa cgaaaaagtg
     9661 ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc
     9721 ctgcacgacc caccaatcgc taaggtggaa gcggtagtaa aacgtgaagg ataacgttgc
     9781 atcagcaacg gcccgaaggg cgagacgaag tcgagtcatc ctgcacgacc caccactaac
     9841 atagttagtt gtagtatcca gcgtagtatt gggtgattag ctcagctggg agagcatctc
     9901 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaccgg gtcgttagct
     9961 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
    10021 accagtttta gcatcgaaga cagatgttaa gcgtgtagga taacgttgcg tcagcaacgg
    10081 cccgtagggc gagcgaagcg agtcatcctg cacgacccac cactaattac ggtgggttcg
    10141 gtggaagtag tttgtagtat ccagcgcagt atcgggtgat tagctcagct gggagagcac
    10201 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac tcgggtcgtt
    10261 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    10321 acccaccaat tttaacatca aactcagatg ttaagcgtga aggataacgt tgcgccagca
    10381 acggcccgta gggcaagcga agcgagtcat cctgcacgac acaccaatct taaagattgg
    10441 ccccgagtag aaatctttca ggtaacaccc gtatgggtcg ttagctcagt tggtaga
//
LOCUS       DAZFKM010000160        10337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFKM010000160 DAZFKM010000000
VERSION     DAZFKM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            208..1602
                     /gene="galP"
                     /locus_tag="ACHKAY_004396"
     CDS             208..1602
                     /gene="galP"
                     /locus_tag="ACHKAY_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO5702955.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1679..2176
                     /locus_tag="ACHKAY_004397"
     CDS             1679..2176
                     /locus_tag="ACHKAY_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO5702956.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2271..2978
                     /gene="endA"
                     /locus_tag="ACHKAY_004398"
     CDS             2271..2978
                     /gene="endA"
                     /locus_tag="ACHKAY_004398"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO5702957.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3058..3789
                     /gene="rsmE"
                     /locus_tag="ACHKAY_004399"
     CDS             3058..3789
                     /gene="rsmE"
                     /locus_tag="ACHKAY_004399"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO5702958.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3802..4752
                     /gene="gshB"
                     /locus_tag="ACHKAY_004400"
     CDS             3802..4752
                     /gene="gshB"
                     /locus_tag="ACHKAY_004400"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO5702959.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4861..5424
                     /locus_tag="ACHKAY_004401"
     CDS             4861..5424
                     /locus_tag="ACHKAY_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO5702960.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5424..5840
                     /gene="ruvX"
                     /locus_tag="ACHKAY_004402"
     CDS             5424..5840
                     /gene="ruvX"
                     /locus_tag="ACHKAY_004402"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO5702961.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6016..6996)
                     /locus_tag="ACHKAY_004403"
     CDS             complement(6016..6996)
                     /locus_tag="ACHKAY_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO5702962.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            7014..7718
                     /gene="yggS"
                     /locus_tag="ACHKAY_004404"
     CDS             7014..7718
                     /gene="yggS"
                     /locus_tag="ACHKAY_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO5702963.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7736..8302
                     /gene="yggT"
                     /locus_tag="ACHKAY_004405"
     CDS             7736..8302
                     /gene="yggT"
                     /locus_tag="ACHKAY_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO5702964.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8299..8589
                     /gene="yggU"
                     /locus_tag="ACHKAY_004406"
     CDS             8299..8589
                     /gene="yggU"
                     /locus_tag="ACHKAY_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO5702965.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8597..9190
                     /locus_tag="ACHKAY_004407"
     CDS             8597..9190
                     /locus_tag="ACHKAY_004407"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO5702966.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            9183..10319
                     /gene="hemW"
                     /locus_tag="ACHKAY_004408"
     CDS             9183..10319
                     /gene="hemW"
                     /locus_tag="ACHKAY_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO5702967.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2431 a         2724 c         2727 g         2455 t
ORIGIN      
        1 tgttacattt cttttcagta aagtcttaat tgcagataac agcgtttaat ctatgatgat
       61 ataactcaat tattttcatg cacttaaacc ataactaaga taaatgttag tgtaagcgat
      121 tacactgatg tgatttgctt cacatctttt tacgtcgtac tcacctatct taattcacaa
      181 taaaaaataa ccatattgga gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac
      241 aaggcaatga cttttttcgt ctgcttcctt gccgctctgg cgggattact ctttggcctg
      301 gatatcggtg taattgctgg cgcactgccg tttattgcag atgaattcca gattacttcg
      361 cacacgcaag aatgggtcgt aagctccatg atgttcggtg cggcagtcgg tgcggtgggc
      421 agcggctggc tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg cgcaattttg
      481 tttgttgccg gttcgctgtt ctctgcggct gcgccaaacg ttgaagtact gattctttcc
      541 cgcgttctgc tggggctggc ggtgggtgtg gcctcttata ccgcaccact gtacctgtca
      601 gaaattgcgc cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt gatgatcact
      661 atcgggatcc tcggtgctta tctttctgat accgccttca gctacaccgg tgcatggcgc
      721 tggatgctgg gtgtgattat catcccggca attttgctgc tgattggtgt cttcttcctg
      781 ccagacagcc cacgttggtt tgccgccaag cgccgttttg ttgatgccga acgcgtgctg
      841 ctacgcctgc gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt
      901 ttgcaggtta aacagagtgg ctgggcgctg tttaaagaga acagcaactt ccgccgcgcg
      961 gtgttccttg gcgtactgtt gcaggtaatg cagcaattca ccgggatgaa cgtcatcatg
     1021 tattacgcgc cgaaaatctt cgaactggcg ggttatacca acactaccga gcaaatgtgg
     1081 gggaccgtga ttgtcggcct gaccaacgta cttgccacct ttatcgcaat cggccttgtt
     1141 gaccgctggg gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc tgctggcatg
     1201 ggcgtactcg gtacaatgat gcatatcggt attcactctc cgtcagcgca gtatttcgcc
     1261 atcgccatgc tgctgatgtt tattgtcggt tttgccatga gtgccggtcc gctgatttgg
     1321 gtactgtgct ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc
     1381 gccaccaact ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg
     1441 ctgggtaacg ccaacacctt ctgggtgtat gcggctctga acgtactgtt tatcctgctg
     1501 acattgtggc tggtaccgga aaccaaacac gtttcgctgg aacatattga acgtaatctg
     1561 atgaaaggtc gtaaactgcg cgaaatcggc gctcacgatt aatctcctca ggcttcctcc
     1621 catcgcggag gaagccacct cttgcagtca tcttttcttc gctctatcct ctgccgctat
     1681 gaaaacatcc cgtctcccta tcgccatcca gcaggccgtt atgcgccgcc tgcgggaaaa
     1741 actcgcccag gccaacctga agctagggcg taactacccg gagccaaaac tctcttacac
     1801 ccagcgcgga acctccgccg gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc
     1861 cgttttgctg ttggaaaaca gtgaagcttt tattgaagaa gtggtaccgc acgaactggc
     1921 acatttgctg gtatggaaac atttcggccg cgtagcgcca catggcaaag agtggaagtg
     1981 gatgatggaa agcgtgctgg gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc
     2041 cgtgcgtcgc aacaccttcc cctaccgctg caagtgccag gagcatcagc ttaccgtacg
     2101 ccgccataat cgcgtagttc gtggcgaggc cgtctatcgc tgtgttcact gcggtgaaca
     2161 gctggttgcg aaataaccat ctgaactatc aggaactttc ctgatctggc tgattgcata
     2221 ccaaaacaac tttcgctacg ttgctggctc gttttaacac ggagtaagtg atgtaccgtt
     2281 atttgtctat tgctgcggtg gtactgagcg cagcattttc cggcccggcg ttggccgaag
     2341 gtatcaatag tttttctcag gcgaaagccg cggcggtaaa agtccacgct gacgcgcccg
     2401 gtacgtttta ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc
     2461 aatcgtgcgg ctatcaggtg cgcaaaaatg aaaaccgagc cagccgcgta gagtgggaac
     2521 acgtcgttcc cgcctggcag ttcggtcacc agcgccagtg ctggcaggac ggtggacgta
     2581 aaaactgcgc taaagatccg gtctatcgca agatggaaag cgatatgcat aacctgcaac
     2641 cgtcagtcgg cgaggtgaat ggcgatcgcg gcaactttat gtacagccag tggaatggcg
     2701 gcgaaggcca gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa gctgccgaac
     2761 caccagcgcg tgcacgcggt gccattgcac gcacctactt ctatatgcgc gaccaataca
     2821 acctgacact ctctcgccag caaacgcagc tgttcaacgc atggaacaag atgtatccgg
     2881 ttaccgactg ggagtgcgag cgcgatgaac gcatcgcgaa ggtgcaaggc aatcataatc
     2941 cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc tacactagcg ggactctttt
     3001 tgttaacccc taccccacgc gtacaaccgc gtggggagac gacgcggatt tttaactatg
     3061 cgtatccccc gcatttatca tcctgaacca ctgaccagcc attctcacat cgcgctttgc
     3121 gaagatgccg ccaaccatat cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa
     3181 ttgtttgacg gtagcaacca ggtctttgac gccgaaatta ccagcgccag caaaaaaagc
     3241 gtggaagtga aggtgctgga aggcaagatc gacgatcgcg aatctccgct gcatattcac
     3301 ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta cgatccagaa atcgatcgaa
     3361 ctcggtgtaa gcctcattac gccacttttt tctgagcgct gcggcgttaa actggatagt
     3421 gaacgtctga acaagaagct ccagcagtgg cagaagattg caattgctgc ctgtgagcag
     3481 tgtggtcgta accgggtgcc ggaaatccgc ccagcgatgg atctggaagc ctggtgtgca
     3541 gagcaggatg aaggactgaa actgaatctt cacccgcgcg ccagtaacag catcaatacg
     3601 ttgccgttac cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg tttatcggca
     3661 gatgaaattg ccatgactgc ccgctatcaa tttactgata tcctgttggg acctcgcgtt
     3721 ttgcgtacag agacaactgc gctcaccgcc attaccgcgc tacaagtacg atttggcgat
     3781 ttgggctaac ggagaagaat aatgatcaag ctcggcatcg tgatggaccc catcgcaaac
     3841 atcaacatca agaaagattc cagtttcgct atgttgctgg aagcacagcg tcgtggttac
     3901 gaacttcact atatggagat gggcgatctg tatctgatca atggcgaagc ccgcgcccat
     3961 acccgcacgc tgaacgtgaa gcagaactac gaagagtggt tttcgttcgt cggtgaacag
     4021 gatctgccgc tggccgatct cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc
     4081 gagtttatct acgcgaccta tattctggaa cgtgccgaag agaaagggac gctgatcgtt
     4141 aacaagccgc agagcctgcg cgactgtaac gagaaactgt ttaccgcctg gttctctgac
     4201 ttaacgccag aaacgctggt tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa
     4261 cacagcgaca tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg
     4321 aaagaaggcg atccaaacct cggcgtgatt gccgaaaccc tgactgagca tggcactcgc
     4381 tactgcatgg cgcaaaatta cctgccagcc attaaagatg gcgacaaacg cgtgctggtg
     4441 gtggatggcg agccggtacc gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt
     4501 ggcaatctgg ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa
     4561 atcgcccgtc agatcgggcc gacgttgaaa gaaaaagggc tgatttttgt tggtctggat
     4621 atcatcggcg accgtctgac tgaaattaac gtcaccagcc caacctgtat tcgtgagatt
     4681 gaagcagagt ttccggtgtc gatcaccgga atgttaatgg atgccatcga agcacgttta
     4741 cagcagcagt aacccacctt agcgagaagg atctcgttga gactctgagt gacagcgccc
     4801 ttctttccac gcatactggg cgctgttgct tttttgaacc aggaaacaga acctctgaca
     4861 atgaatttac agcatcactt tcttattgcc atgcctgctc tccaggatcc gattttccgt
     4921 cgttccgtgg tctacatttg cgaacataat accaatggtg caatggggat catcgtcaac
     4981 aagccgctgg aaaatctcaa aattgaaggg attctggaaa agctgaagat cacgccggag
     5041 ccgcgtgatg aatcaatccg tctggataaa ccggttatgc tcggcggtcc gctggctgaa
     5101 gatcgcgggt ttattttgca tactccgccc tccaattttg cttccagcat tcgcatttca
     5161 gacaacacag tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa
     5221 ccgtctgacg tattggtggc tctgggttat gcctcctggg aaaaaggtca actggaacaa
     5281 gaaattctcg ataacgcgtg gctaacggcc ccggcggatc tgaatattct gttcaaaacg
     5341 ccgattgccg accgctggcg cgaggcggca aaactgattg gtgtggatat tctcaccatg
     5401 cctggtgtgg cagggcacgc ctgatgagtg gaaccttact cgccttcgac ttcggcacca
     5461 aaagcattgg cgtagcggtc ggccaacgca ttaccggcac tgctcgccct ttgcctgcaa
     5521 ttaaagcaca ggacggtacg ccggactgga acctcatcga acgtttactg aaagagtggc
     5581 agccggacga aatcatcgtc ggtttgccgc tgaatatgga cggcaccgag caaccattga
     5641 ctgccagagc gcgtaaattt gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc
     5701 tccatgacga acgtcttagc accgtggaag cccgttccgg tctgtttgaa cagggcggct
     5761 atcgggcgct caacaaaggc aaagtcgact ctgcctctgc ggttattatt ctcgaaagct
     5821 atttcgagca gggatattaa ggcgatctaa aagcctggcg gatagtcgtt accgccatct
     5881 tgccggatgc ggcgtgaacg ccttatccag cacacatctg gcagcggctg taggtctgat
     5941 aagacgcgtt agcgtcgcat cagacattta ttgcctttat aggccctata aacttgcgca
     6001 tcgggcatgg caacgtcaca aacgcccttc ccccacccgc tgctgataac tctgctgaaa
     6061 cgttaacatc cccacctgct gcccggtttg aataacatgc ggtaactggt gggttttccc
     6121 ttcgcgaatc aaattcccca ccgcgggagt gttaatcaat aattcaaaca gcgccacgcg
     6181 tccttcctgt ttatccactt ccagcttttg tgacaacacg gcccgtaaac tacctgccag
     6241 ttgattacgt acgggatctt tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc
     6301 ctgcgcggca ccacgcgtat gtaatgttgc cagcaccaaa tgcccggttt ctgccgccgt
     6361 cagtgccaga cggattgtct cgctgtcacg cagctctccg agcaaaatca catcaggatc
     6421 ttcccgcaat gcggcccgca atcccgatgc gaacgtcata cagtgcaaac caatttcccg
     6481 ctgctggatc aaacatcgct ggctggcata gatatattca acaggatctt ccagcgtcag
     6541 aatatgcgca tcggcatgtt gatagagata gccaaccatc gccgctagcg tggtagattt
     6601 gccgctcccc gtcgcccccg tcaccagaat caggccattc tcgctcttga gtaattccgg
     6661 caataccgtt ggtgcgccaa gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa
     6721 tgccagagaa ataccttgcc gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc
     6781 cagcgacacg gcaaaatcca gctggccatt ttccagcaat attgcccgct gatcgtcatc
     6841 cagccactcc cgcagtagct cttcgacgtc cggcgcatca aacggcgcag cttccattag
     6901 cccgcgaatg cgccatcgtg cggaccaggc gctgcacagg tgtagatccg agacgttatg
     6961 ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg aaaatgaacg
     7021 atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca acgcgttgcg
     7081 gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca
     7141 tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt caggaagggg
     7201 tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg cattttattg
     7261 gcccgttgca gtctaataaa agccgcctgg tggcagagca tttcgactgg tgtcatacca
     7321 tcgaccgttt gcgcatcgct acccgcctca acgatcagcg cccggcagaa cttccccctc
     7381 ttaacgtact gattcaaatt aatattagtg atgaaaacag taagtccggg attcaactgg
     7441 cagaactgga cgagctggca gctgcggtcg ctgaactacc gcgtttacgt ctgcgcgggc
     7501 tgatggcaat ccctgcacct gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa
     7561 tggctgtagc atttgccgga ctgaaaacgc gctacccgca tatcgacacg ctctctctgg
     7621 gaatgtcgga cgatatggaa gccgccattg cggcaggtag cacgatggtt cgtatcggca
     7681 ctgcaatttt tggtgcgcgt gattactcta aaaaataagg aattaaagga acgccatgaa
     7741 tacgttgact ttcctgcttt caacggtcat tgagctgtat accatggtgc tgttattacg
     7801 catctggatg cagtgggctc attgtgattt ttacaacccc ttctcacagt ttgtagtgaa
     7861 ggtaacgcag ccaattatcg ggccactgcg ccgcgttatt ccggcaatgg gccccattga
     7921 cagcgcctcg ctgctggttg cctatattct cagttttatc aaagccatcg tgctgtttaa
     7981 agtggtgacc ttcctgccaa tcatctggat tgccggttta ctgattctgc tgaaaaccat
     8041 cggcctgctg attttctggg tcctgctggt gatggcgatt atgagctggg taagccaggg
     8101 tcgtagcccg attgaatacg tgctgattca gctggccgat ccgctgctgc gcccgattcg
     8161 ccgcctgcta ccggcaatgg gtgggattga tttctcgccg atgatcctcg ttctgctgct
     8221 gtatgtcatc aatatgggtg tcgcagaagt attacaggca accggaaaca tgctgctgcc
     8281 ggggctgtgg atggcgttat gagtgccgta acagttaatg atgacggtct ggttttacgg
     8341 ctctatattc agccgaaagc cagccgtgat tctattgtcg gtttacatgg cgacgaagtt
     8401 aaagtcgcca ttaccgcgcc gccggttgac ggtcaggcca acagtcatct ggtgaagttt
     8461 ctcggtaagc aattccgggt tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc
     8521 cgccacaaac aaattaaaat cattaatccg caacaaatcc cgccagaaat cgcggcgtta
     8581 attaattagg tatcctatgc aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg
     8641 tgagctggcg tcgctgctta gcgacttcgg tcttgatatc gtggcccaaa cagacctcgg
     8701 cgtagattcc gctgaagaaa ccggcctgac ctttatcgaa aacgcgattc tgaaagcgcg
     8761 ccatgcggca aaagtgaccg gtttaccggc aattgccgac gactctggtc tggcggtaga
     8821 tgtgcttggc ggcgcgccgg gcatttactc cgcgcgttat tccggtgaag acgcgaccga
     8881 tctaaagaat ctgcaaaaac tgctggaaac actgaaagac gtaccggatg accaacgtca
     8941 ggcgcgtttc cactgcgtgc tggtatatct gcgtcacgcg gaagatccga ctccgctggt
     9001 atgccacggt agctggccgg gcgtgattac tcgtgaacca gcgggcactg gtggctttgg
     9061 ttatgatcca atcttcttcg taccttccga agggaaaacc gccgccgaac tgacccacga
     9121 agaaaagagc gccatttccc accgtggtca ggcattgaaa ctgctgctgg acgctttacg
     9181 taatggttaa attaccgccg ctgagtctct acattcacat cccgtggtgc gtgcagaaat
     9241 gcccgtactg cgatttcaac tctcacgcgt tgaaaggaga agtgccgcac gacgattacg
     9301 ttcagcatct gctttgcgat ctggacaacg atgtggctta cgctcagggc cgtgaagtaa
     9361 agacaatctt tattggcggt ggtacgccga gcctgctttc cggcccggcg atgcaaacgc
     9421 tgctggacgg cgtgcgtgcg cgtttgccgc tggcagcgga tgcagaaatt actatggaag
     9481 cgaaccctgg tacggtagaa gccgatcgct ttgtcgatta tcagcgtgct ggtgtgaacc
     9541 gcatctctat tggtgtgcag agttttagcg aagacaagct gaaacgactt gggcgtattc
     9601 atggcccgca agaagcgaaa cgcgcggcaa agctggcgag cgggctgggg ctgcgtagct
     9661 ttaacctcga tttgatgcat ggactaccgg atcaatcact ggaagaggcg cttggcgatc
     9721 tacgccaggc cattgaactg aatccgccgc atctttcctg gtatcaactg accatcgaac
     9781 ctaatacgct gtttggttca cgcccgccgg tactgccgga cgacgacgcg ctatgggata
     9841 tattcgaaca ggggcatcag ttattaaccg cagcgggtta tcagcaatat gaaacttccg
     9901 cttacgccaa atccagttat cagtgccagc acaatctcaa ctactggcgc tttggcgact
     9961 acatcggtat tggctgcggc gcgcacggca aagtgacctt cccggatggg cgcattctgc
    10021 gtaccactaa aacgcgtcat ccgcgtggtt ttatgcaagg aaggtatctg gaaagccagc
    10081 gtgatgtcga agccgcagat aagccgtttg agttctttat gaatcgcttc cgtctgctgg
    10141 aggccgcgcc gcgcgtggag tttattgcgt ataccgggct ttgcgaagat gtgattcgcc
    10201 cacagttaga cgaggcgatt gcccagggtt atctcaccga atgtgcggat tactggcaga
    10261 taacggaaca tggtaaatta ttcttaaact cacttcttga attattcctc gctgagtaat
    10321 cattaatgcg tctgcgg
//
LOCUS       DAZFKM010000161        10331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFKM010000161 DAZFKM010000000
VERSION     DAZFKM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKAY_004409"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHKAY_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO5702968.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKAY_004410"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHKAY_004410"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO5702969.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKAY_004411"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHKAY_004411"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO5702970.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKAY_004412"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHKAY_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO5702971.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKAY_004413"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHKAY_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO5702972.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKAY_004414"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHKAY_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO5702973.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKAY_004415"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHKAY_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO5702974.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKAY_004416"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHKAY_004416"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO5702975.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKAY_004417"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHKAY_004417"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO5702976.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..>10331
                     /gene="yfiL"
                     /locus_tag="ACHKAY_004418"
     CDS             9972..>10331
                     /gene="yfiL"
                     /locus_tag="ACHKAY_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO5702977.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRL"
BASE COUNT         2646 a         2733 c         2427 g         2525 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa ccagctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catctgtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt a
//
LOCUS       DAZFKM010000162        10315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFKM010000162 DAZFKM010000000
VERSION     DAZFKM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..297)
                     /locus_tag="ACHKAY_004419"
     CDS             complement(34..297)
                     /locus_tag="ACHKAY_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5702978.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(329..1345)
                     /gene="lplA"
                     /locus_tag="ACHKAY_004420"
     CDS             complement(329..1345)
                     /gene="lplA"
                     /locus_tag="ACHKAY_004420"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO5702979.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(1373..2017)
                     /locus_tag="ACHKAY_004421"
     CDS             complement(1373..2017)
                     /locus_tag="ACHKAY_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO5702980.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            2123..3091
                     /gene="serB"
                     /locus_tag="ACHKAY_004422"
     CDS             2123..3091
                     /gene="serB"
                     /locus_tag="ACHKAY_004422"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO5702981.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            3140..4522
                     /gene="radA"
                     /locus_tag="ACHKAY_004423"
     CDS             3140..4522
                     /gene="radA"
                     /locus_tag="ACHKAY_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO5702982.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4543..5775
                     /gene="nadR"
                     /locus_tag="ACHKAY_004424"
     CDS             4543..5775
                     /gene="nadR"
                     /locus_tag="ACHKAY_004424"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO5702983.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(6082..7749)
                     /gene="ettA"
                     /locus_tag="ACHKAY_004425"
     CDS             complement(6082..7749)
                     /gene="ettA"
                     /locus_tag="ACHKAY_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO5702984.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            7960..9897
                     /gene="sltY"
                     /locus_tag="ACHKAY_004426"
     CDS             7960..9897
                     /gene="sltY"
                     /locus_tag="ACHKAY_004426"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO5702985.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            9987..10313
                     /gene="trpR"
                     /locus_tag="ACHKAY_004427"
     CDS             9987..10313
                     /gene="trpR"
                     /locus_tag="ACHKAY_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO5702986.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         2328 a         2669 c         2922 g         2396 t
ORIGIN      
        1 aaaatccatt caagtatcta ttcacgaatc tattcattta tgttcagccg tcatccagga
       61 agggcatatc agcaactctg gtggacaacc ataaatttct gccattttgc ttttgatgtt
      121 tgagctaagc cgttccgtct tttcgatctg ttttagagaa ttaatcgaga tacctaacat
      181 tttacttact tcagcaagcg ataatttatg cctgatacgc acggcggcta gccaggaaat
      241 acgctggctt aacattaaat tgataatctc ccgctcctgg ttttccagat cgatcatatt
      301 cccttccttt ggtattttaa tcagcgagtt accttaccgc ccccgctatc cacgtcgaca
      361 actcccgtag ctctttttcc tggtccggga agtcaaccaa cagcgcttcg cactcttgtt
      421 gcagcatatc cgcgcggtac aggcagcctt gcagtcgccc ggcgagggct tccagcggcg
      481 cggggttgag gctgtcggta aacacctggg cgcgggtgat atggcctttt tcaacgtcga
      541 aatgcagttc cacgccgccc cagctaaagc gttcatccag cagatgcgag aatgccggag
      601 cctgaccgaa gttccattcc cagctactct gacgggcaaa ggtttcggcg aagtttggca
      661 agtctggcgt tttgtccggg gagatgattt ccgcttctac acgctcgcca taatgggcga
      721 aaaaggcctt ggttatgacc tcgcaaacct gctcatgggt gatccccggc agcagctcgg
      781 tgaggttggt cacgcgggaa cgcactgagg taatgccttt cgccgccagt ttctttttat
      841 ccggattgag atagtttgcc aggcggctaa ggtcggcatt gagcagcaaa gtgccgtggt
      901 ggaagccacg atctttggtt tcgcgatagg ccgagcctga gactttgcgg tcgccttcga
      961 ctgttttcac caccagatcg ttacgcccgg acgcttcggc gctgacgccg agcgcgttca
     1021 gcgcattgag cacaatcgac gtagagatgg ttttatcgta ctccggcttg ccagccataa
     1081 aggtaaagca ggtattgccg agatcgtgga acaccgcgcc gccaccgcta ctgcgccgcg
     1141 ccaggcggac gttatcttct tccatccgcc gggtattaca ctctttccac gggttctgcg
     1201 cgcgaccaat cactaccgtg tcggcattgc gccagagaaa cagaacgcgc tgcgtggcgg
     1261 gcatttggcg aaaaatacac tcttccaccg ccaggttaaa ccacgggtcg taagagtcag
     1321 agatgagcag gcgtaatgtg gacataacga tttcctttct tgtagtaatg ggtcactctt
     1381 ttttctcgct ttcttcctct tccggcaccg gtttgctggc ggttaacagg aagggcgatt
     1441 gctgccagcg ggtgcgttta ccctgtagca gcgtgcgggt cagcactacg ccgattgcca
     1501 gtgagagcag caacatcagg cgtaaaatgt tagtggtgtt atccacctgt tgggcttcgg
     1561 tggcgagcgt atgggtgtcg agtgtcaggc gcagatagcc gagcggtccg tttttacccg
     1621 caattggctc gacaatctgc tggttaaaat agccgcctgc ttttttaccg tcgagcgcca
     1681 gccggtcgcg cacttcgacg ctttcgcctg aacgggcgat aagatcgcct tgttcgtcat
     1741 acacacccgc gtcgaggatg cggctttcat ccgttaactg atcgaggatc gcctgaatgc
     1801 gtttttcatc tggtgagtcg gtacgcatca gcggtgcaac gttcagcgtc acctgacgcg
     1861 ccagggtgcg ggccagttct tccagctgtg gattacgctg tcgctggtgg ttttgactaa
     1921 accatgacgc cccctgcatc agtgccacta acaaggcaag acagaacagg acaatcactg
     1981 cccgatgcag ccggaatttc agttttgtgc gagccatctt ccaccctttg aaaatttgag
     2041 acttaatgtt gccagaagca atggatacaa ggtagcctca tgcgttattt tccctgcttc
     2101 gaacgatttt acaggagcct taatgcctaa cattacctgg tgcgacctgc ctgaagatgt
     2161 ctctttatgg ccgggtctgc ctctttcatt aagtggtgat gaagtgatgc cactggatta
     2221 ccacgcaggt cgtagcggct ggctgctgta tggtcgtggg ctggataaac aacgcctgac
     2281 ccaataccag agcaaactgg gcgcggcgat ggtgattgtt gccgcctggt gcgtggaaga
     2341 ttatcaggtg attcgtctgg caggttcact caccgcgcgc gctacgcgcc tggcccatga
     2401 agcgcaactg gatgtcgcgc cgctggggaa aatcccgcac ctgcgcacgc cgggtttgct
     2461 ggtgatggac atggattcca ccgccatcca gattgaatgt attgatgaaa ttgccaaact
     2521 ggccggaacg ggcgagatgg tggcggaggt aaccgaacgg gcgatgcgcg gcgaactcga
     2581 ttttaccgcc agcctgcgca gccgcgtggc gacgctgaaa ggcgctgatg ccaatattct
     2641 gcaacaggtg cgtgaaaatc tgccgctgat gccaggctta acgcagctgg tgctcaagct
     2701 ggaaacgctg ggctggaaag tggcgattgc ctccggcggc tttactttct ttgctgaata
     2761 cctgcgcgac aagctgcgcc tgaccgccgt ggtagccaat gaactggaga tcatggacgg
     2821 taaatttacc ggcaatgtga tcggcgacat cgttgacgcg cagtacaaag cgaaaactct
     2881 gacccgcctc gcgcaggagt atgaaatccc gctggcgcag accgtggcga ttggcgatgg
     2941 agccaatgac ctgccgatga tcaaagcggc agggctgggg attgcctacc atgccaagcc
     3001 aaaagtgaat gaaaagacgg aagtcaccat ccgtcacgct gacctgatgg gggtattctg
     3061 catcctctct ggcagcctga atcagaagta attgcctgcc cgtcatcctg cgggcggcac
     3121 agcattaacg aggtacaccg tggcaaaagc tccaaaacgc gcctttgttt gtaatgaatg
     3181 cggggccgat tatccgcgct ggcaggggca gtgcagtgcc tgtcatgcct ggaacaccat
     3241 caccgaggtg cgtcttgctg cgtcgccaac ggtggcgcgt aacgagcgtc tcagtggcta
     3301 tgccggtagc gccggggtgg caaaagtcca gaaactctcc gacatcagcc ttgaagagct
     3361 gccgcgtttt tccaccggat ttaaagagtt cgaccgcgta ttaggcggcg gcgtggtacc
     3421 gggaagtgcc attctgattg gcggtaaccc aggcgcgggg aaatccacgc tgttgctgca
     3481 aacgctgtgc aaactggccc agcagatgaa aacgctgtat gtcaccggcg aagagtcgct
     3541 gcaacaggtg gcaatgcgcg ctcatcgcct tggcctgccg actgacaatc tcaatatgtt
     3601 gtcggaaacc agcatcgagc agatctgcct gattgctgaa gaagagcaac cgaagctgat
     3661 ggtaattgac tccatccagg tgatgcatat ggcggatgta cagtcatcgc ctggtagcgt
     3721 ggcgcaggtg cgtgaaacgg cggcttattt gacacgcttc gccaaaacgc gcggtgtggc
     3781 gattgtcatg gttggacacg taaccaaaga tggctcgctg gcgggcccta aagtgctgga
     3841 acactgtatc gactgttcgg tgcttttgga tggcgatgcc gactcccgtt ttcgcacctt
     3901 gcgcagccat aaaaaccgct tcggcgcggt gaatgagctg ggcgtcttcg cgatgaccga
     3961 acaggggctg cgtgaagtca gcaacccttc ggcaattttc ttaagtcgcg gagatgaagt
     4021 gacctccggc agctccgtga tggtggtgtg ggaaggaacg cgtccgttgc tggtggagat
     4081 tcaggcgctg gtcgatcact cgatgatggc gaatccgcgc cgcgtggcgg taggtctgga
     4141 gcaaaaccgt ctggcaatcc tgctggctgt cttgcaccgt cacggtggtc tgcaaatggc
     4201 cgatcaggat gtatttgtga acgtggtcgg tggtgtgaag gtaaccgaaa ccagtgccga
     4261 cttagcgtta ctgctggcga tggtttccag cctgcgtgac agaccgctac cgcaggatct
     4321 ggtggtgttt ggtgaagtcg ggctggcagg ggagatccgc ccggtgccca gcggtcagga
     4381 gcgaatctct gaagcggcga aacacggttt tcgccgggcg attgttccgg cggctaacgt
     4441 gccgaaaaaa gcgccggaag ggatgcagat ttttggcgtt aaaaaactct ccgacgcgct
     4501 tagcgtgttc gacgacttat aatgagagat acggagggag atatgtcgtc atttgattac
     4561 ctgaaaactg ccatcaagca acagggctgc acgttacagc aggtagctga tgccagcggt
     4621 atgaccaaag ggtatttaag ccagttactg aatgccaaaa ttaaaagccc cagcgcgcaa
     4681 aagctggagg cgttgcaccg ttttttgggg cttgaatttc cccggcagaa gaaaaccatt
     4741 ggtgtcgtat tcggtaagtt ctacccgctg cataccggac atatctacct tatccagcgc
     4801 gcctgtagcc aggttgacga gctgcatatc attatgggtt ttgacgatac ccgcgaccgc
     4861 gcgttgtttg aagacagcgc catgtcgcag cagcccacgg tgccggatcg tctgcgctgg
     4921 ttattgcaaa cttttaaata tcagaaaaat attcgcattc atgctttcaa cgaagagggc
     4981 atggagccgt atccgcatgg ctgggatgtg tggagcaacg gcatcaaaaa gtttatggct
     5041 gaaaagggga tccagccgga cctgatctac acctcggaag aagccgatgc gccacagtat
     5101 atggaacatc tggggatcga gacggtactg gtcgatccga aacgtacctt tatgagtatc
     5161 agcggtgcgc agatccgcga aaacccgttc cgctactggg aatatattcc tacggaagtg
     5221 aagccgttct ttgtgcgtac cgtggcgatc cttggcggcg agtcgagcgg taaatccacc
     5281 ctggtaaaca aacttgccaa tatcttcaat accaccagtg cgtgggaata tggtcgcgat
     5341 tatgtctttt cacacctcgg cggtgatgag atcgcattgc agtattccga ctacgataaa
     5401 atcgcgctgg gccacgcaca atacattgat tttgcagtga aatacgccaa taaagtggcg
     5461 tttattgaca ctgattttgt cactacccag gcgttctgca aaaagtacga agggcgtgag
     5521 catccgttcg tgcaggcgtt gattgatgaa taccgtttcg atctggtgat cctgctggag
     5581 aacaacacgc cgtgggtggc ggatggttta cgcagcctcg gcagttcggt ggatcgcaaa
     5641 gagttccaga acttgctggt ggagatgctg gaagagaaca atatcgaatt cgtgcgggtt
     5701 gaagaggacg attatgacag ccgtttcctg cgctgcgtgg agctggtgcg ggagatgatg
     5761 ggggagcaga gataaccgcg atgaaacggc tcaaaggcga ggtataaaat aagttttttg
     5821 atgagttgaa aacctttctg gaagaggctg tcgagattaa acaaggtttg aaaaaacctg
     5881 cacgggtgac ccgctacgaa attgcggata ctaaggctgt tgtaggccgg aaaaggcgtt
     5941 cacgccgtat ccggcattcg gtgctcaatg cctgatgcga cgcttaccgc gtcttatcag
     6001 gcctggagat tcacaacgta ttgaaatttc ttggtttgta ggccggataa ggcgttcacg
     6061 ccgcatccgg cattttacgc attacttcgc aatacgcttg tacttgatac gcttcggctc
     6121 cagtgcgtct gcgcccagcg tgcgtttctt gtactcttcg tactcagtaa agttaccttc
     6181 gaagaactca actttacctt catcctggta gtccaggatg tgcgtggcga tacggtcgag
     6241 gaaccaacgg tcgtgcgaga taaccatcgc acagcccggg aactccagca gggcgttttc
     6301 cagcgcgcgc agggtttcga tatccaggtc gttggttggt tcgtcgagca gcagcatgtt
     6361 gccgccaacc tgcagcagct tcgccagatg cagacgaccg cgctcaccac cggaaagttc
     6421 accaacgcgt ttaccctgat caaccccttt aaagttaaag cggccaacgt aggcgcggct
     6481 tggcatctcg gtgttgccga tcttcataat atccagcccg ccggaaactt cttcccaaac
     6541 ggttttgctg ttatccattg agtcacggaa ctgatcaacc gatgccagtt tcaccgtttc
     6601 acctaaagtg atggtgccgc tgtccggctg ttcctgacca gagatcatac ggaacagggt
     6661 cgatttaccc gcgccgttcg gaccgatgat cccgacgatt gcccctttcg ggatcgagaa
     6721 gctcaggtca tcaatcagca ggcgatcacc gtaggattta cgcaggttgc tgacttccag
     6781 cactttatcg cccagacgcg gtccaggtgg aataaacagt tcgttggttt cgttacgttt
     6841 ctgatattcg gtgctgttca gctcttcaaa gcgtgccaga cgtgctttac ctttcgactg
     6901 gcggccttta gttccctgac gtacccactc cagctctttc tcgatcgact tacgacgcgc
     6961 cgcttcttgt gaagcttcct gcgccaggcg ctgatctttc tgctccagcc aggaggagta
     7021 gttgccttcc cacggaatac cttcaccgcg gtcaagttcg aggatccagc ctgcaacgtt
     7081 atcgaggaag taacggtcgt gggtaatcgc caccacggtg ccctcgaagt cgtgcaggaa
     7141 gcgttccagc caggccacgg attcggcatc caggtggttg gtcggttcgt cgagcagcag
     7201 catgtctggt ttttccagca gcaggcggca caacgctacg cgacgacgct caccaccgga
     7261 gaggttagcg attttcgcgt cccagtccgg cagacgtagc gcatccgccg cacgctccag
     7321 ctgtacgttc aggttatgac cgtcgtgagc ctgaatgatc tcttccagac ggccttgttc
     7381 agcggccagc ttgtcaaaat cggcatccgg atcggcgtac agcgcataca cttcatccag
     7441 gcgtttcagg gcgttaacca cttcagaaac cgcttcttca atggactcac gcacggtgtg
     7501 ttccgggttc agctgcggtt cctgcggcag gtaaccaatc ttgatgtctg gctgcggacg
     7561 cgcttcacct tcgatgtctt tatcaatgcc cgccataatg cgcagcaggg tagacttacc
     7621 cgcgccgttc agacccagga caccaatttt tgccccaggg aagaaactca gagagatgtt
     7681 tttcaaaata tgacgtttcg gcggaacaac tttgccgaca cgatgcatgg tataaacgaa
     7741 ttgagccacg ttggacttcg cctctatgtt tatcgtgatg atgagttttc aaaggcgaag
     7801 tgtagccttt ttccccgcct aatcccagtt ggtcgatcac cttcgcagta aaagtaaaaa
     7861 agtgtccgta acgtggcgta aacggcaatg actggttagc ataaatctat tacgcggcat
     7921 gacgctgcat tgatgtattt acacttagag gatgcgcttg tggaaaaagc caaacaagtt
     7981 acctggcggc tgttggctgc cggtgtctgt ctgctgacgg tcagcagcgt ggcgcgagcc
     8041 gactcactgg atgagcagcg tagccgttac gcgcaaatca agcaggcctg ggataatcga
     8101 caaatggatg tggtcgaaca aatgatgcct ggactgaagg attatccgct ttatccctac
     8161 ctggaatacc gccagatcac cgatgatctg atgaatcaac cggcggtgac ggtcactaac
     8221 tttgttcgcg ctaaccccac gcttcctccc gctcgcacgc tgcaatctcg tttcgtcaat
     8281 gaactggcgc ggcgtgaaga ctggcgtggc ttgttagcct ttagcccgga aaagcccgga
     8341 actaccgaag cgcaatgtaa ttactactat gcgaaatgga acaccgggca gagtgaagaa
     8401 gcctggcaag gggcgaaaga gctgtggcta accggcaaga gccagcctaa cgcctgtgac
     8461 aagttattta gcgtctggcg tgcgtcaggt aaacaagatc cgctggctta tttagagcgt
     8521 atccgtctgg cgatgaaagc gggtaacact ggcctggtaa cagtgctggc agggcagatg
     8581 cctgccgatt accagactat cgcctcggca atcatttcac tggcgaacaa ccctaatacg
     8641 gtactgacct tcgcgcgtac aaccggagcg accgatttta cccgtcaaat ggcggcggtg
     8701 gcgtttgcca gtgtggcgcg gcaggatgct gaaaatgcac ggctgatgat cccgtcgctt
     8761 gcccaggcgc agcagcttaa tgaagatcat attcaggagc tgcgcgatat cgtcgcctgg
     8821 cgtttgatgg gcaacgatgt caccgacgag caggcgaaat ggcgcgatga cgccattatg
     8881 cgctcgcaat ccacgtcgtt gattgaacgc cgtgtgcgaa tggcacttgg caccggcgat
     8941 cgtcgcggcc tgaatacctg gctggcgcgt ctgccgatgg aagcaaaaga gaaagatgaa
     9001 tggcgttact ggcaggcgga tttattgctg gaacgcggac gtgaagctga agcgaaagag
     9061 attttgcatc aactcatgca acagcgtggt ttctacccga tggttgcagc acaacgcatc
     9121 ggagaagagt atgagctgaa gattgataaa gcgccgcaga atgttgacag cgccctgact
     9181 caggggccgg agatggcgcg cgtgcgcgag ttgatgtact ggaatctcga taacaccgcg
     9241 cgtagcgagt gggccaatct ggtgaagagc aagtcaaaaa cagagcaggc tcaactggct
     9301 cggtatgctt tcaataacca atggtgggat cttagcgttc aggcaacgat cgccgggaag
     9361 ctttgggatc atctggaaga gcgattcccg ctggcttaca acgatctttt caaacgttac
     9421 accagtggga aagagatccc gcaaagctat gcgatggcga ttgcccgtca ggagagcgcc
     9481 tggaatccga aagtgaaatc accggtaggg gccagcggcc tgatgcagat tatgcctggt
     9541 acagcgaccc atacggtgaa gatgttctct attcctggtt atagcagccc tgggcaattg
     9601 ctggatccgg aaacgaatat caacattggc accagttacc tgcaatatgt ttatcagcag
     9661 tttggcaata accgtatttt ctcctcagca gcttataacg ccggacctgg acgggtgcga
     9721 acctggcttg gcaacagcgc cgggcgtatc gacgctgtgg catttgtcga gagtattccg
     9781 ttctccgaga cgcgcggtta tgtgaagaac gtgctggctt atgacgctta ctaccgctat
     9841 ttcatggggg ataaaccgac gttgatgagc accacggaat ggggacgtcg ttactgatcc
     9901 gcacgtttat gatatgctat cgtactcttt agcgagtaca accgggggag gcattttgct
     9961 tcccccgcta acaatggcga catattatgg cccaacaatc accctattca gcagcgatgg
    10021 cagaacagcg tcaccaggag tggttacgtt ttgtcgacct gcttaagaat gcctaccaaa
    10081 acgatctcca tttaccgttg ttaaacctga tgctgacgcc agatgagcgc gaagcgttgg
    10141 ggactcgcgt gcgtattgtc gaagagctgt tgcgcggcga aatgagccag cgtgagttaa
    10201 aaaatgaact cggcgcgggc atcgcgacga ttacgcgtgg atctaacagc ctgaaagccg
    10261 cgcccgttga gctgcgccag tggctggaag aggtgttgct gaaaagcgat tgatt
//
LOCUS       DAZFKM010000163        10269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFKM010000163 DAZFKM010000000
VERSION     DAZFKM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            273..1646
                     /gene="aroP"
                     /locus_tag="ACHKAY_004428"
     CDS             273..1646
                     /gene="aroP"
                     /locus_tag="ACHKAY_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO5702987.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1689..2543)
                     /gene="ampE"
                     /locus_tag="ACHKAY_004429"
     CDS             complement(1689..2543)
                     /gene="ampE"
                     /locus_tag="ACHKAY_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO5702988.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2540..3091)
                     /gene="ampD"
                     /locus_tag="ACHKAY_004430"
     CDS             complement(2540..3091)
                     /gene="ampD"
                     /locus_tag="ACHKAY_004430"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO5702989.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3179..4072
                     /gene="nadC"
                     /locus_tag="ACHKAY_004431"
     CDS             3179..4072
                     /gene="nadC"
                     /locus_tag="ACHKAY_004431"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO5702990.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4275..4715
                     /gene="ppdD"
                     /locus_tag="ACHKAY_004432"
     CDS             4275..4715
                     /gene="ppdD"
                     /locus_tag="ACHKAY_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO5702991.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4725..6110
                     /gene="gspE"
                     /locus_tag="ACHKAY_004433"
     CDS             4725..6110
                     /gene="gspE"
                     /locus_tag="ACHKAY_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO5702992.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6100..7302
                     /gene="hofC"
                     /locus_tag="ACHKAY_004434"
     CDS             6100..7302
                     /gene="hofC"
                     /locus_tag="ACHKAY_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO5702993.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7337..8380)
                     /gene="guaC"
                     /locus_tag="ACHKAY_004435"
     CDS             complement(7337..8380)
                     /gene="guaC"
                     /locus_tag="ACHKAY_004435"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO5702994.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8536..8580)
                     /locus_tag="ACHKAY_004436"
     CDS             complement(8536..8580)
                     /locus_tag="ACHKAY_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5702995.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8605..9225
                     /gene="coaE"
                     /locus_tag="ACHKAY_004437"
     CDS             8605..9225
                     /gene="coaE"
                     /locus_tag="ACHKAY_004437"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO5702996.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9225..9968
                     /gene="zapD"
                     /locus_tag="ACHKAY_004438"
     CDS             9225..9968
                     /gene="zapD"
                     /locus_tag="ACHKAY_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO5702997.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9978..10175
                     /gene="yacG"
                     /locus_tag="ACHKAY_004439"
     CDS             9978..10175
                     /gene="yacG"
                     /locus_tag="ACHKAY_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO5702998.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2525 a         2695 c         2627 g         2422 t
ORIGIN      
        1 ttaattcaat taaaacgaat ttaaattcat tctacatatt gagaggggtt gaggctgagc
       61 tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc gggtgcattc
      121 gctgcctcat accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct
      181 ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt
      241 agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc
      301 agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag
      361 ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca gggattatcc
      421 tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg
      481 tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt
      541 ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt gccatggctg
      601 agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc acctgggttt
      661 ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg
      721 gcgagatgga gttctggttt gccattatca aagttatcgc ggtggtagcg atgatcatct
      781 tcggcggctg gctgctattc agtggcaacg gcggcccgca ggcgaccgtt agcaacctgt
      841 gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg atggcgatga
      901 tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc
      961 cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg attttctata
     1021 ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca
     1081 gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca
     1141 tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta
     1201 tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac
     1261 gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg tgcgtactga
     1321 ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg gtatctgcac
     1381 tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc gccaagcagg
     1441 aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc
     1501 tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg
     1561 tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa
     1621 ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca gggtgagggc
     1681 aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac cacgaccacg
     1741 aacgaggttt tcttcgccat tgaaaccgct gccctcggcg tctccacctt atcgacatgc
     1801 ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg
     1861 aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc gatcaaggca
     1921 tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg
     1981 ccggactgta aacgatgatg cggcgtctga tatcgcgcca gccagtattg ccatgcacgc
     2041 aaaaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat cagccagaac
     2101 agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa
     2161 tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc
     2221 gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga
     2281 cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag tagcgtggga
     2341 acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata
     2401 atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca
     2461 agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc
     2521 agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcacacga aaccgtgccc
     2581 aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca
     2641 tgtttttagc gatatccgga tagcaatcaa tcagtgcccg cgtaaccgcc gcaagctgtt
     2701 gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc ccaatagaaa
     2761 aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat gcacgtttat
     2821 cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc
     2881 gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata
     2941 atgcgtcgat ccacggaccg ccaaactcgc ctggcggaag gctaatattg tgcaccacca
     3001 gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg
     3061 cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata
     3121 cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat
     3181 gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct
     3241 cgatatcccc ggcgcggtag cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc
     3301 caacaatgat attacggcaa aacttttacc ggaaaattct cgctctcatg ccacggtgat
     3361 cacccgcgag aatggcgtct tttgcggcaa acgctgggtt gaagaggtgt ttattcaact
     3421 ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaatgccaa
     3481 tcaatccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc
     3541 gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt
     3601 gctggaaggc accaacacgc agttgttgga tacgcgcaaa accttacccg gcctgcgttc
     3661 agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga
     3721 tgccttcctg atcaaagaaa accatattat tgcctccggc tcagtgcgcc aggcggtcga
     3781 aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga
     3841 acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga
     3901 acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa
     3961 cgtcactgac aaaacactgc gtgaatttgc cgaaacgggc gtggacttta tctccgtcgg
     4021 tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct aatagttgac
     4081 agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc
     4141 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaatc tgctttcccg
     4201 tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcgc caactaaatc
     4261 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
     4321 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
     4381 aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
     4441 gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc
     4501 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
     4561 ggcaagaaag tctcaatggg ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg
     4621 gcgtcaccgg ctggacgcgc aactgcaata ttcaaagtga cagcgcattg catcaagcct
     4681 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
     4741 tcactgccct gtgtctgcgt tatcatggag tcttgctgga tgccagcgaa gaggtggttc
     4801 atgttgcggt agtcgatgca ccttcgcatg agctactgga cgcattgcat ttcgctacca
     4861 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac gccagtcgca
     4921 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctaa
     4981 ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc gaaccagcgg
     5041 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
     5101 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg
     5161 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
     5221 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac
     5281 agcaggtgag ccaggcactg gatgtcaaca cgcttggaat gcagccgtta caactggcgg
     5341 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
     5401 gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaataccgct gacattaata
     5461 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc
     5521 atccgcgtgc cggactcacc tttcagggcg tgttgcgtgc gttattgcgc caggatcctg
     5581 acgtcatcat gatcggagag atccgcgatg gcgaaacagc agagatcgct attaaagcgg
     5641 cgcaaactgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc gaaacgctgg
     5701 tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc atcggcgctt acgctggtaa
     5761 tagcccagcg tctggtacgc aaactttgcc cacattgtcg ccggcagcaa ggggaaccca
     5821 tccacattcc agacaatgta tggccatcgc cgctgcccca ctggcaggca cccggttgtg
     5881 tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg cccataacgc
     5941 cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgcac
     6001 gacaggcggg tatgcgtacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa
     6061 ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcgagtaa gcaactctgg
     6121 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc
     6181 cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccacttag cctgaagcga
     6241 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
     6301 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct ggcggaacag
     6361 catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct cgaacagggc
     6421 attgcttttt ccaatgcctt attaccctgg tcagaggtat ttccgccact ctatcaggcg
     6481 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt
     6541 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatc
     6601 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt tctgccggag
     6661 tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca ggggatcatg
     6721 acgctggcag actttagtgg cgaatggagc tggctgctgg tgttgttcgg ctttctgctg
     6781 gcgatagcca ataagttgct gatgcgccga ccgacctggc ttatagtgcg gcagaaattg
     6841 ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac gcagattttt
     6901 acgattctgg cgctgacaca aagtgcaggc attacttttt tgcagggcgt agagagcgtc
     6961 agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc
     7021 agtaacggtc atcccatctg gctggcgcta aaaaataccg gggagtttag cccgctctgt
     7081 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg acctcatgtt agacaacctc
     7141 gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa
     7201 ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc gatgtatctg
     7261 ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg tgttgctcca
     7321 caccagcgtt ggaagattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga
     7381 taaatgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg
     7441 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
     7501 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
     7561 cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
     7621 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag catgacgaaa tcggcaccgc
     7681 cgccaaaggc ttttgcgaca tcgcccggcg tggtgcagcc accgtcgctg acgatcattc
     7741 cgcccagacc gtgcgccgca tcggcacatt cgattaccgc agaaagctgc ggataaccga
     7801 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
     7861 cagcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg ccagcacaaa
     7921 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
     7981 aaccattcgc cacatcaata caaacgaagt ttaatgccgg gttcaggtcg agaatctgtt
     8041 tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt ttcagcacat
     8101 cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt ttatgcacag
     8161 cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtacct acggtgtcca
     8221 tatttgcggc gataattggc acgccggacc agctctgacc tgaatgtttg aaggtgaatt
     8281 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
     8341 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
     8401 tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa tcaatgcggc
     8461 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
     8521 tgagcgaacg tttcatcaat ttcgctttcg tgcactcatt tttaacgttt tgataatcat
     8581 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
     8641 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
     8701 gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat
     8761 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
     8821 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
     8881 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact gtgggttgtg
     8941 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
     9001 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg cgagcatgtc
     9061 gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc agatgacgtc
     9121 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
     9181 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
     9241 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
     9301 agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
     9361 atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
     9421 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc gtggaccaga
     9481 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atttccgcgc
     9541 cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
     9601 gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
     9661 cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgctcaccc
     9721 aggcacttac catggtgctg gatttaattc gccagtcggc tcccttccgt aaacaaacca
     9781 gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt
     9841 cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt gccattcgtt
     9901 ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc gaactggcct
     9961 gttgctaagg agtgaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
    10021 cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga
    10081 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    10141 aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc gcacgtagat
    10201 actgttatgg cctgcaagtt ttttcctggt ccggtttccc ggattgcagg catgccagac
    10261 acagcgccg
//
LOCUS       DAZFKM010000164        10180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFKM010000164 DAZFKM010000000
VERSION     DAZFKM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..856)
                     /gene="yaaA"
                     /locus_tag="ACHKAY_004440"
     CDS             complement(80..856)
                     /gene="yaaA"
                     /locus_tag="ACHKAY_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO5702999.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(926..2356)
                     /locus_tag="ACHKAY_004441"
     CDS             complement(926..2356)
                     /locus_tag="ACHKAY_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO5703000.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2635..3588
                     /gene="tal"
                     /locus_tag="ACHKAY_004442"
     CDS             2635..3588
                     /gene="tal"
                     /locus_tag="ACHKAY_004442"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO5703001.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3703..4290
                     /gene="mog"
                     /locus_tag="ACHKAY_004443"
     CDS             3703..4290
                     /gene="mog"
                     /locus_tag="ACHKAY_004443"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO5703002.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4325..4891)
                     /gene="satP"
                     /locus_tag="ACHKAY_004444"
     CDS             complement(4325..4891)
                     /gene="satP"
                     /locus_tag="ACHKAY_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO5703003.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="ACHKAY_004445"
     CDS             complement(5040..5753)
                     /gene="msyB"
                     /locus_tag="ACHKAY_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO5703004.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5779..6183)
                     /locus_tag="ACHKAY_004446"
     CDS             complement(5779..6183)
                     /locus_tag="ACHKAY_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO5703005.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6560..8476
                     /gene="dnaK"
                     /locus_tag="ACHKAY_004447"
     CDS             6560..8476
                     /gene="dnaK"
                     /locus_tag="ACHKAY_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO5703006.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8565..9695
                     /gene="dnaJ"
                     /locus_tag="ACHKAY_004448"
     CDS             8565..9695
                     /gene="dnaJ"
                     /locus_tag="ACHKAY_004448"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO5703007.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="ACHKAY_004449"
     CDS             complement(9799..9951)
                     /gene="mokC"
                     /locus_tag="ACHKAY_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO5703008.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2659 a         2558 c         2640 g         2323 t
ORIGIN      
        1 aattctgcaa tatattgaat ctgcatgctt ttgtaggccg gataaggcgt tcacgccgca
       61 tccggcattg actgcaaact taacgctgct cgtagcgttt aaacaccagt tcgccattgc
      121 tggaggaagc ttcatcaaag aagtaacctt cgctattaaa accagtcagt tgctctggtt
      181 tggtcagccg attttcaata ataaaacgac tcatcagacc gcgtgctttc ttagcgtaga
      241 agctgattat cttaaatttg ccgttcttct catcgaggaa caccggcttg ataatctcgg
      301 cattcaattt cttcggcttc accgatttaa aatactcatc tgacgccaga ttaatcacca
      361 cattatcgcc ttgtgctgcg agcgcctcgt tcagcttgtt ggtgatgata tctccccaga
      421 attgatacag atctttccct cgggcattct caagacggat ccccatttcc agacgataag
      481 gctgcattaa atcgagcggg cgcagtacgc catacaagcc ggaaagcatt cgcaaatgct
      541 gttgggcaaa atcgaaatcg tcttcgctga aggtttcggc ctgcaagccg gtgtagacat
      601 cacctttaaa cgccagaatc gcctggcggg cattctccgg cgtgaaatct ggctgccagt
      661 catgaaagcg agcggcgttg atacccgcca gtttgtcgct gatgcgcatc agcgtgctaa
      721 tctgcggagg cgtcagtttc cgcgcttcat ggatcaactg ctgggaattg tctaacagct
      781 ccggcagcgt atagcgcgtg gtggtcaacg ggctttggta atcaagcgtt ttcgcaggtg
      841 aaataagaat cagcatatcc agtccttgca ggaaatttat gccgacttta gcaaaaaaag
      901 agaatgagtt gatcgatagt tgtgattact cctgcgaaac atcatcccac gcgtccggag
      961 aaagctggcg gccgatatcc ggataacgca acggatcaaa caccgggcgc acgccgagtt
     1021 tacgctggcg tagataatca ctggcaatgg tatgaaccac aggcgagagc agtaaaatgg
     1081 cggtcaaatt ggtaatagcc atgcaggcca ttatgatatc tgccagttgc cacatcagcg
     1141 gaagacttag caaggtgccg ccgatgaccg ttgcgaaggt gcagatccgc aaacaccaga
     1201 tcgctttagg gttgttcagg cgtaaaaaga agagattgtt ttcggcgtaa atgtagttgg
     1261 caacgatgga gctgaaggca aacagaataa ccacgagggt aacaaactca gcaccccagg
     1321 aacccattag cacccgcatc gccttctgga taagctgaat accttccagc ggcatgtagg
     1381 ttgtgccgtt acccgccagt aatatcagca tggcgcttgc cgtacagatg accagggtgt
     1441 cgataaaaat gccaatcatc tggacaatcc cttgcgctgc cggatgcgga ggccaggacg
     1501 ccgctgccgc tgccgcgttt ggcgtcgacc ccattcccgc ctcattggaa aacatactgc
     1561 gctgaaaacc gttagtaatc gcctggctta aggtatatcc cgccgcgccg cctgccgctt
     1621 cctgccagcc aaaagcactc tcaaaaatag accaaatgac gtggggaagt tgcccgatat
     1681 tcattacgca aatcaccagg ctggtcagta cccagattat cgccatcaac gggacaaacc
     1741 cctgcatgag ccgggcgacg ccatgaagac cgcgagtgat cgccagcaga gcaaagacag
     1801 cgagaataat acctgtcacc agcgggggaa aatcaaaaga aaaactcagg gctcgcgcaa
     1861 cggcgttcgc ctgaattccg ctgaaaatta tgccgtaggc gatgagcaaa aagacggcga
     1921 acagaacgcc catccagcgc atccccagcc cgcgcgccat ataccatgcc ggtccgccac
     1981 gaaactgccc attggcatca cgttctttat aaagctgtgc gagggaacat tcggcaaacg
     2041 aggtcgccat gccgataaac gcggcaaccc acatccagaa gacggctcca ggtccaccgg
     2101 cggtaattgc cagcgcaacg ccggccaggt tgccgctacc cacgcgcgcc gcaagactgg
     2161 tacacaatga ctgaaatgag gttaaaccgc ctggctgtgg atgaatgcta tttttaagac
     2221 ttttgccaaa ctggcggatg tagcgaaact gcacaaatcc ggtgcgaaaa gtgaaccaac
     2281 aacctgcgcc gaagagcagg taaatcatta ccgatcccca aaggacgctg ttaataaagg
     2341 agaaaaaatc tggcatgcat atccctctta ttgccggtcg cgatgacttt cctgtgtaaa
     2401 cgttaccaat tgtttaagaa gtatatacgc tacgaggtac ttgataactt ctgcgtagca
     2461 tacatgaggt tttgtataaa aatggcgggc gatatcaacg cagtgtcaga aatccgaaac
     2521 agtctcgcct ggcgataacc gtcttgtcgg cggttgcgct gacgttgcgt cgtgatatca
     2581 tcagggcaga ccggttacat ccccctaaca agctgtttaa agagaaatac tatcatgacg
     2641 gacaaattga cctcccttcg tcagtacacc accgtagtgg ccgacactgg ggacatcgcg
     2701 gcaatgaagc tgtatcaacc gcaggatgcc acaaccaacc cttctctcat tcttaacgca
     2761 gcgcagattc cggaataccg taagttgatt gatgatgctg tcgcctgggc gaaacagcag
     2821 agcaacgatc gcgcgcagca gatcgtggac gcgaccgaca aactggcagt aaatattggt
     2881 ctggaaatcc tgaaactggt tccgggccgt atctcaactg aagttgatgc gcgtctttcc
     2941 tatgacaccg aagcgtcaat tgcgaaagca aaacgcctga tcaaactcta caacgatgct
     3001 ggtattagca acgatcgtat tctgatcaaa ctggcttcta cctggcaggg tatccgtgct
     3061 gcagaacagc tggaaaaaga aggcatcaac tgtaacctga ccctgctgtt ctccttcgct
     3121 caggctcgtg cttgtgcgga agcgggcgtg ttcctgatct cgccgtttgt tggccgtatt
     3181 cttgactggt acaaagcgaa taccgataag aaagagtacg ctccggcaga agatccgggc
     3241 gtggtttctg tatctgaaat ctaccagtac tacaaagagc acggttatga aaccgtggtt
     3301 atgggcgcaa gcttccgtaa catcggcgaa attctggaac tggcaggctg cgaccgtctt
     3361 accatcgcac ctacactgct gaaagagctg gctgagagcg aaggggctat cgaacgtaaa
     3421 ctgtcttaca ccggcgaagt gaaagcgcgt ccggcgcgta tcactgagtc cgagttcctg
     3481 tggcagcaca accaggatcc aatggcagta gataaactgg cggaaggtat ccgtaagttt
     3541 gctattgacc aggaaaaact ggaaaaaatg atcggcgatc tgctgtaatc attcttagcg
     3601 tgaccgggaa gtcggtcacg ctacctcttc tgaagcctgt ctgtcactcc cttcgcagtg
     3661 tatcattctg tttaacgaga ctgtttaaac ggaaaaatct tgatgaatac tttacgtatt
     3721 ggcttagttt ccatctctga tcgcgcatcc agcggcgttt atcaggataa aggcatccct
     3781 gcgctggaag aatggctgac atcggcgcta accacgccgt ttgaactgga aacccgctta
     3841 atccccgatg agcaggcgat catcgagcaa acgttgtgtg agctagtgga tgaaatgagt
     3901 tgccatctgg tgctcaccac gggcggaact ggcccggcgc gtcgtgacgt aacgcccgat
     3961 gcgacgctgg cagtagcgga ccgcgagatg cctggctttg gtgaacagat gcgccagata
     4021 agcctgcatt ttgtaccaac tgcgatcctt tcgcgtcagg ttggtgtgat tcgcaaacag
     4081 gcgctgatcc ttaacttacc cggtcagccg aagtctatta aagagacgct ggaaggtgtg
     4141 aaggacgctg cgggtaacgt tgtggtacac ggtatttttg ccagcgtacc gtactgcatt
     4201 cagttgctgg aagggccata cgttgaaacg gcaccagaag tggttgctgc attcagaccg
     4261 aagagtgcaa gacgcgacgt tagcgaataa aaaaatcccc ccgagcgggg ggatctcaaa
     4321 acaattagtg ggattcacca atcggcagaa cggtgcgacc aaactgctcg ttcagtactt
     4381 cacccatcgc cagatagatt gcactggcac cacagatcag accaatccag ccggcaaagt
     4441 ggatgattgc ggcgttaccg gcaatgttac cgatcgccag cagggcaaac agcacagtca
     4501 ggctaaagaa aacgaattgc agaacgcgtg cgcctttcag cgtgccgaag aacataaaca
     4561 gcgtaaatac gccccacaga cccaggtaga caccaaggaa ctgtgcattt ggcgcatcgg
     4621 tcagacccag tttcggcatc agcagaatcg caaccaacgt cagccagaaa gaaccgtaag
     4681 aggtgaatgc ggttaaaccg aaagtgttgc cttttttgta ctccagcaga ccggcaaaaa
     4741 tttgcgcgat gccgccgtag aaaatgccca tggcaagaat aataccgtcc agagcgaaat
     4801 aacccacgtt gtgcaggtta agcagaatgg tggtcatgcc gaagcccatc aggcccagcg
     4861 gtgccggatt agccaactta gtgttgccca taattcctca aaaatcatca tcgaatgaat
     4921 ggtgaaataa tttccctgaa taactgtagt gttttcaggg cgcggcataa taatcagcca
     4981 gtggggcagt gtctacgatc ttttgagggg aaaatgaaaa ttttccccgg tttccggtat
     5041 cagacctgag tggcgctaac catccggcgc aggcaggcga tttgcagtac ggctggaatc
     5101 gtcacgcgat aggcgctgcc gctgaccgct ttaaccccat ttagtgccgc acctacaggg
     5161 cctcccagcc ccgcgccgcg cagcaaacca tgcccaagta cgctcattgc tgcgtgggtg
     5221 cgtaaaatgc gggtcagttg gctggaaagc aaatgcgaca cgccttttgc caataatttg
     5281 tctttcatca gcagcggcag cagctcttcc agctcattca ccctcgcatc gaccgcgtgc
     5341 agaaactcct gtttatgttc ctcgtccata ttcttccagg tgttacgcag aaattgttcc
     5401 agtaactgtt gctcaatctc aaacgtagac atctctttgt cggctttcag cttcaatcgt
     5461 tttgaaacat cgagcaaaat ggcccgatac aatttaccgt gtccgcgcag tttgttggcg
     5521 atactatcgc caccaaaatg ctgtaattct ccagcaatca gctgccagtt gcggcgatgt
     5581 tgctcgggat gcccttccat agatttaaac agttcgttgc gcatcagtac gctggagagg
     5641 cgagttttgc ctttttcatt atgggtgagc aatcgggcga aatttgccaa ctgttcctca
     5701 ctgcaatgct ggagaaaatc cagatctgaa tcattcaggt aattaacatt cattttttgt
     5761 ggcttctata ttctggcgtt agtcgtcgcc gataattttc agcgtggcca tatccgatga
     5821 gttcaccgta tgaccggaaa aggtgatttt tgagacgcag cgtttattgt cgttatcgct
     5881 gttaatgttg atccagtcag tggtttgccc ttcttttatt tctgaaggaa tattcaggct
     5941 ctgactggcg ctacgggcgg ctttgaaata aaccgatgca ccgcttaact gtaaatcgcc
     6001 atggtcggca gagagttgta tgcgtttaac aattcggcaa acaggaagtt tcagcgccag
     6061 atcgttggtt tcgttacgtg gcatcgcaat gacgccgagg attttatggt cgtttgcctg
     6121 cgccgtgcag cacagcatca gactaatcgc caggctggcg gaaatcgtaa aaacggattt
     6181 cataaggatt ctcttagtgg ggagaggtag ggggatggat acccactagt ttactgctga
     6241 taaagagaag attcaggtgc gtaatgtttt ctttttatta caattttttg atgaatgtct
     6301 tggctgcgat tcattcttta tatgaataaa attgctgtca gtttcacgtc ttgtcctgct
     6361 atatcgcgaa atttctgcgc aaaagcacaa aaaatttttg catctccccc ttgatgacgt
     6421 gggttacgac cccatttagt agtcaaccgc agtgagtgag tctgcaaaaa aatgaaattg
     6481 ggcagttgaa accagacgtt tcgcccctat tacagactca caaccacatg atgaccgaat
     6541 atatagtgga gacgtttaga tgggtaaaat aattggtatc gacctgggta ctaccaactc
     6601 ttgtgtagcg attatggatg gcaccactcc tcgcgtgctg gagaacgccg aaggcgatcg
     6661 caccacgcct tctattattg cctataccca ggatggtgaa actctggttg gtcagccggc
     6721 taaacgtcag gcagtgacga acccgcaaaa caccctgttt gcgattaaac gcctgattgg
     6781 ccgccgcttc caggacgaag aagtacagcg tgatgtttcc atcatgccgt tcaaaattat
     6841 tgctgctgat aacggcgacg catgggtcga agttaaaggc cagaaaatgg caccgccgca
     6901 gatttctgct gaagtgctga aaaaaatgaa gaaaaccgct gaagattact tgggtgaacc
     6961 ggtaactgaa gctgttatca ccgtaccggc atactttaac gatgctcagc gtcaggcaac
     7021 caaagacgca ggccgtatcg ctggtctgga agtaaaacgt atcatcaacg aaccgaccgc
     7081 agctgcgctg gcttacggtc tggacaaagg tactggcaac cgtactatcg cggtttatga
     7141 cctggggggt ggtactttcg atatttctat tatcgaaatc gacgaagttg acggcgaaaa
     7201 aaccttcgaa gttctggcta ccaacggtga tacccacctg ggtggtgaag acttcgacag
     7261 ccgtctgatc aactacctgg ttgaagaatt caagaaagat cagggcattg acctgcgcaa
     7321 cgatccgctg gcaatgcagc gcctgaaaga agcggcagaa aaagcgaaaa tcgaactgtc
     7381 ttccgctcag cagaccgacg ttaacctgcc gtacatcact gcagatgcga ccggtccgaa
     7441 acacatgaac atcaaagtga ctcgtgcgaa actggaaagc ctggttgaag atctggtaaa
     7501 ccgttccatt gagccgctga aagttgcact gcaggacgct ggcctgtccg tatctgatat
     7561 cgacgacgtt atcctcgttg gtggtcagac tcgtatgcca atggttcaga agaaagttgc
     7621 tgagttcttt ggtaaagagc cgcgtaaaga cgttaacccg gacgaagctg tagcaatcgg
     7681 tgctgctgtt cagggtggtg ttctgactgg tgacgtgaaa gacgtactgc tgctggacgt
     7741 taccccgctg tctctgggta tcgaaaccat gggcggtgtg atgacgacgc tgatcgcgaa
     7801 aaacaccact atcccgacca agcacagcca ggtgttctct accgctgaag acaaccagtc
     7861 tgcggtaacc atccatgtgc tgcagggtga acgtaaacgt gcggctgata acaaatctct
     7921 gggtcagttc aacctggatg gtatcaaccc ggcaccgcgc ggcatgccgc agatcgaagt
     7981 taccttcgat atcgatgctg acggtatcct gcacgtttcc gcgaaagata aaaacagcgg
     8041 taaagagcag aagatcacca tcaaggcttc ttctggtctg aacgaagatg aaatccagaa
     8101 aatggtacgc gacgcagaag ctaacgccga agctgaccgt aagtttgaag agctggtaca
     8161 gactcgcaac cagggcgacc atctgctgca cagcacccgt aagcaggttg aagaagcagg
     8221 cgacaaactg ccggctgacg acaaaactgc tatcgagtct gcactgactg cactggaaac
     8281 tgctctgaaa ggtgaagaca aagccgctat cgaagcgaaa atgcaggagc tggcacaggt
     8341 ttcccagaaa ctgatggaaa tcgcccagca gcaacatgcc cagcagcaga ctgccggtgc
     8401 tgatgcttct gcaaacaacg cgaaagatga cgatgttgtc gacgctgaat ttgaagaagt
     8461 caaagacaaa aaataatcgc cctataaacg ggtaattata ctgacacggg cgaaggggaa
     8521 tttcctctcc gcccgtgcat tcatctaggg gcaatttaaa aaagatggct aagcaagatt
     8581 attacgagat tttaggcgtt tccaaaacag cggaagagcg tgaaatcaaa aaggcctaca
     8641 aacgcctggc catgaaatac cacccggacc gtaaccaggg tgacaaagag gccgaggcga
     8701 aatttaaaga gatcaaggaa gcttatgaag ttctgaccga ctcgcaaaaa cgtgcggcat
     8761 acgatcagta tggtcatgct gcgtttgagc aaggtggtat gggcggcggc ggttttggcg
     8821 gcggcgcaga cttcagcgat atttttggtg acgttttcgg cgatattttt ggcggcggac
     8881 gtggtcgtca acgtgcggcg cgcggtgctg atttacgcta taacatggag ctcaccctcg
     8941 aagaagctgt acgtggcgtg accaaagaga tccgcattcc gactctggaa gagtgtgacg
     9001 tttgccacgg tagcggtgca aaaccaggta cacagccgca gacctgtccg acctgtcatg
     9061 gttctggtca ggtgcagatg cgtcagggat tcttcgctgt acagcagacc tgtccacact
     9121 gtcagggccg cggtacgctg atcaaagatc cgtgcaacaa atgtcatggt catggtcgtg
     9181 ttgagcgcag caaaacgctg tccgttaaaa tcccggcagg ggtggacact ggagaccgca
     9241 tccgtcttgc gggcgaaggt gaagcgggcg agcatggcgc accggcaggc gatctgtacg
     9301 ttcaggttca ggttaaacag cacccgattt tcgagcgtga aggcaacaac ctctattgcg
     9361 aagtcccgat caacttcgct atggcggcgc tgggtggcga aatcgaagta ccgacccttg
     9421 atggtcgcgt caaactgaaa gtgcctggcg aaacccagac cggtaagctt ttccgtatgc
     9481 gcggtaaagg cgtcaagtct gtccgcggtg gcgcacaggg tgatttgctg tgccgcgttg
     9541 tcgtcgaaac accggtaggc ctgaacgaga agcagaaaca gctgctgcaa gagctgcaag
     9601 aaagcttcgg tggcccaacc ggtgagcaca acagcccgcg ctcaaagagc ttctttgatg
     9661 gtgtgaagaa gttttttgac gacctgaccc gctaacctcc ccaaaagcct gcccgtgggc
     9721 aggcttgggt aaaattaggg tgcgttgaag atatgcgagc acctgaaaag tggcggggat
     9781 cactccccgc cgttgcactt actcggattc gtaagccgtg aaaacagcaa cctccgtctg
     9841 gccagttcgg atgtgaacct cacagaggtc ttttctcgtt accagcgccg ccactacggc
     9901 ggtgatacag atgacgatca gggcgacaat catcgcctta tgctgcttca ttgctctctt
     9961 ctccttgacc ttacggtcag taagaggcac tctacatgtg ttcagcatat agggggcctc
    10021 gggttgatag taaaatatca ctcggggctt ttctctatct gccgttcagc taatgcctga
    10081 gacagatagc ctcaagtacc cgccgctatt atatcgctct ctttaaccca ttctgtttta
    10141 tcgattctaa tcctgaagac gcctcgcatt tttgtggcgt
//
LOCUS       DAZFKM010000165        10127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFKM010000165 DAZFKM010000000
VERSION     DAZFKM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..669
                     /gene="ftnB"
                     /locus_tag="ACHKAY_004450"
     CDS             166..669
                     /gene="ftnB"
                     /locus_tag="ACHKAY_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO5703009.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(748..999)
                     /gene="yecJ"
                     /locus_tag="ACHKAY_004451"
     CDS             complement(748..999)
                     /gene="yecJ"
                     /locus_tag="ACHKAY_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO5703010.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(1114..1200)
                     /gene="azuC"
                     /locus_tag="ACHKAY_004452"
     CDS             complement(1114..1200)
                     /gene="azuC"
                     /locus_tag="ACHKAY_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO5703011.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1463..1786
                     /gene="yecR"
                     /locus_tag="ACHKAY_004453"
     CDS             1463..1786
                     /gene="yecR"
                     /locus_tag="ACHKAY_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO5703012.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1957..2454
                     /gene="ftnA"
                     /locus_tag="ACHKAY_004454"
     CDS             1957..2454
                     /gene="ftnA"
                     /locus_tag="ACHKAY_004454"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO5703013.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2492..2731)
                     /gene="yecH"
                     /locus_tag="ACHKAY_004455"
     CDS             complement(2492..2731)
                     /gene="yecH"
                     /locus_tag="ACHKAY_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO5703014.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2922..4133
                     /gene="tyrP"
                     /locus_tag="ACHKAY_004456"
     CDS             2922..4133
                     /gene="tyrP"
                     /locus_tag="ACHKAY_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO5703015.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4195..4860)
                     /gene="yecA"
                     /locus_tag="ACHKAY_004457"
     CDS             complement(4195..4860)
                     /gene="yecA"
                     /locus_tag="ACHKAY_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO5703016.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5056..5142)
                     /locus_tag="ACHKAY_004458"
     tRNA            complement(5056..5142)
                     /locus_tag="ACHKAY_004458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5106..5108),aa:Leu,seq:taa)
     gene            complement(5155..5228)
                     /locus_tag="ACHKAY_004459"
     tRNA            complement(5155..5228)
                     /locus_tag="ACHKAY_004459"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5194..5196),aa:Cys,seq:gca)
     gene            complement(5283..5358)
                     /locus_tag="ACHKAY_004460"
     tRNA            complement(5283..5358)
                     /locus_tag="ACHKAY_004460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5323..5325),aa:Gly,seq:gcc)
     gene            complement(5510..6058)
                     /gene="pgsA"
                     /locus_tag="ACHKAY_004461"
     CDS             complement(5510..6058)
                     /gene="pgsA"
                     /locus_tag="ACHKAY_004461"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO5703017.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6115..7947)
                     /gene="uvrC"
                     /locus_tag="ACHKAY_004462"
     CDS             complement(6115..7947)
                     /gene="uvrC"
                     /locus_tag="ACHKAY_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO5703018.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7944..8600)
                     /gene="uvrY"
                     /locus_tag="ACHKAY_004463"
     CDS             complement(7944..8600)
                     /gene="uvrY"
                     /locus_tag="ACHKAY_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO5703019.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8742..8858
                     /locus_tag="ACHKAY_004464"
     CDS             8742..8858
                     /locus_tag="ACHKAY_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703020.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            8896..9072
                     /gene="yecU"
                     /locus_tag="ACHKAY_004465"
     CDS             8896..9072
                     /gene="yecU"
                     /locus_tag="ACHKAY_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO5703021.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            9059..9283
                     /gene="yecF"
                     /locus_tag="ACHKAY_004466"
     CDS             9059..9283
                     /gene="yecF"
                     /locus_tag="ACHKAY_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO5703022.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9351..10073)
                     /gene="sdiA"
                     /locus_tag="ACHKAY_004467"
     CDS             complement(9351..10073)
                     /gene="sdiA"
                     /locus_tag="ACHKAY_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO5703023.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2544 a         2546 c         2336 g         2701 t
ORIGIN      
        1 cattttctta tacccgttca gacgttattc ttatttcaga tcatcgtcag aattgactcc
       61 acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt atggtgtttt
      121 gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc aaccgctgga
      181 atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa tctctacctt
      241 cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt ccttcgcgcc
      301 caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa gagtgtcggc
      361 gctaccccca tcgttaaagc cattgatgtt cccggtgaaa aactgaactc tctggaagaa
      421 ctgttccaaa aaacgatgga agaatacgag caacgttcta gtacgttggc acagttagcc
      481 gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga tctggaaaaa
      541 gaacagcagc atgatggtct gttgctgcaa accattcttg atgaagtgcg cagtgcgaaa
      601 cttgcgggta tgtgccctgt gcagaccgac caacatgttc tgaatgtcgt gtcacaccag
      661 ctgcattgat catcatcggc gctaatgcat tgcgccgatg aaggttttga gaaaccgcag
      721 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
      781 ttcaataagc gcgatggctt tttggattgc ctgcatggtg accgggtctg cggcggcggg
      841 atggttagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
      901 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag caaccacatc
      961 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     1021 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     1081 gacacaaaaa aacctgccgg agcaggtttt ttattatcgg aacatattgc ctggcggtac
     1141 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     1201 cgcggcactc cgaccatttg ttatacaggt gttattctct ttgcgcttat aatatgacaa
     1261 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     1321 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     1381 gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     1441 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     1501 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     1561 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     1621 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcgg tcccctttgg
     1681 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     1741 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     1801 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     1861 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     1921 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     1981 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     2041 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     2101 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     2161 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     2221 gaaacctata aacacgaaca attaatcacg cagaaaatta acgaactggc tcatgctgca
     2281 atgaccaatc aggactaccc aacatttaat ttcctgcaat ggtatgtttc tgagcagcat
     2341 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     2401 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     2461 caggcggcag gttttctgcc gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg
     2521 tcgaaaaacc ttcttctgaa ggtataaatt tgccttttgc tgccagaaac gctaccagct
     2581 ctcccgcagt catcccttct gccgagcagg tgtgaaaacg tgcctgttca ccaaaacgcg
     2641 ctttaattgc tgcttccaga ctggcatgcg tatattgctc gcctgattca atcatcatat
     2701 ttaacacttc atgaccgtga atagagtcca tcgtccctcc tcaaaaaaag cctagcgtag
     2761 cgattgccgc ttatgaagac tttgcgccag cgcaggactg aatgcttttt attgtacatt
     2821 tatatttaca ccatatgtaa cgtcggtttg acgaagcagc cgttatgcct taacctgcgc
     2881 cgcagatatc actcataaag atcgtcagga cagaagaaag cgtgaaaaac agaaccctgg
     2941 gaagtgtttt tatcgtggcg ggaaccacaa ttggcgcagg catgctggca atgccgctgg
     3001 ctgcggccgg tgttggtttt agcgttacgt taatcttgtt gattgggctt tgggcgttga
     3061 tgtgctacac ggcgctatta ctgctggagg tgtaccagca tgttccggca gataccggtc
     3121 ttggcacgct ggcaaaacgc tatctgggac gctacggtca atggctgacg ggcttcagta
     3181 tgatgttctt aatgtatgct ctgactgcgg catacatcag cggtgccggt gaattgttgg
     3241 cctccagcat cagcgactgg acaggtattt caatgtcggc aaccgctggc gtgctgttgt
     3301 tcacttttgt tgccggtggc gtggtttgtg ttggaacatc gctggtcgat ttatttaacc
     3361 gttttctgtt cagcgccaaa attatttttc tagtggtgat gctggtattg ctgctaccgc
     3421 atattcacaa agtgaatctt ttaaccctgc cgttgcaaca ggggctggct ctgtcagcaa
     3481 tcccggtgat ttttacctca tttggttttc acggtagcgt gccgagtatt gtcagctata
     3541 tggatggcaa cgttcgtaag ctacgctggg tgtttataac cggtagtgcg atccccctgg
     3601 tggcatatat tttctggcag gtggcaacgc ttggcagcat tgattcaaca acctttatgg
     3661 gattgctggc taatcatgct ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg
     3721 cctctccgca tgttgagctg gcagtgcatt tatttgctga tttagccctc gccacgtcat
     3781 ttctcggcgt tgcgttaggc ttatttgatt atctggctga tttatttcag cgttcaaata
     3841 ccgttggtgg acggttgcaa actggtgcaa ttacctttct gccgccgttg gcgtttgcac
     3901 tgttttatcc acgaggattt gtgatggcgc tgggttacgc cggtgtggcg ctggcggtac
     3961 tggcattgat tatcccttcg ctgttgacct ggcaaagcag aaagcacaat cctcaggcgg
     4021 gttaccgggt gaaaggtggt cgtccggcgc tggtggtggt gtttctctgt ggtattgctg
     4081 tgattggcgt gcaatttttg attgcggcag ggttgttacc agaagtgggg tgatcagata
     4141 gcctcaaatt ccttattggg tgccagaatt aacgctgaca cccaatttga cctcttaatg
     4201 caggcagcac tgcttaaatt tcttaccact accgcacggg caaggatcgt tacgccccgg
     4261 tttctcttct gctttgatcg gttgctgaac agctttttcc tgcggatgcg ccatccagta
     4321 cgcatgtaga tcaagcgccg ccagtcgaat ggcatctacg ctctcttcaa acgcttctgg
     4381 cgacattttc tctacccgct cgaagttttc ctcagtaccg tgcagcgcaa tcgcctccag
     4441 cgctggtttt aacgaatcgg gcaacgttga ccagtcagaa agtgccacgc cccgcatata
     4501 gccaaagcac cactcctcaa caatcgtcag ctcgctgcca tcaacttctc gcaagccgaa
     4561 taacggctca aactgctccg ggaattcgtt cagacgctct gcggtatcgg ccatatgttg
     4621 aaaagccaga ttcataaagc gcgtcatctc tttctctgac gcccagcgcg gcacatagtc
     4681 agccccaccc cacacggcaa ccagccactg ttccggttca atctcttgcg gagaactcaa
     4741 caccgccgtc aataaaccgt ccagctccgc cacatcaagg atggcgtggt cagtgttgta
     4801 tttggtcaga atatcgtcca gccattccaa ctcactttcg tttaacggtc ccgttttcat
     4861 acgcttttcc ttgtggatct caactcgcca gcacctatct tacatgccgg tccgtatcag
     4921 agatactttt tgagtggctt tgctggtgat taaaaattag ggagggtgta acgacaagtt
     4981 gcaggcacaa aaaaaccacc cgaaggtggt ttcacgacac tgcttattgc tttgatttta
     5041 ttcttatctt tcccatggta cccggagcgg gacttgaacc cgcacagcgc gaacgccgag
     5101 ggattttaaa tcccttgtgt ctaccgattc caccatccgg gctcgggaag aaagtggagg
     5161 cgcgttccgg agtcgaaccg gactagacgg atttgcaatc cgctacataa ccgctttgtt
     5221 aacgcgccaa attcttcagg cctttcagcc agacatccgc ttgacgccga tgtcttttaa
     5281 actggagcgg gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac
     5341 caactgagct attcccgcat tcatcaagca atcagttaat cacttgattt tattatcgtc
     5401 tggcaatcag tgccgccgtt cgatgcgttg cattctactt acctggcgcg atgagtcaac
     5461 gatatttttc accacttttg atcgtttgct gaaaattacg ccgaaacgat cactgatcaa
     5521 gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga ccacagagtc agtaccgcag
     5581 ccacaaagaa aagtgcaata ccggcgtact caacccaaat gttcggacgc cacagcagcc
     5641 aggccaacgc caccatctgg gcagtggttt tcactttccc aatccaggag acagccacgc
     5701 tactgcgttt acccaactcc gccatccatt cgcgtagcgc agaaataata atttcacggg
     5761 cgatcatcgt tgccgccggt agggtcaccc accagctgtg ataatgctcg gttaccagca
     5821 ccatggcgat agccacgaga actttatctg ctacagggtc aaggaaagca ccaaaccggg
     5881 tactctggtt ccagcggcgt gccagaaaac catcgaacca gtcagtcacc gccgcgacgc
     5941 agaaaatgag cgcggcggca aacggcgacc aggtgacagg cagataaaag accaatacaa
     6001 agaatgggat aaggatgaca cggaacagtg taagcaacgt agggatatta aattgcataa
     6061 tgacgggtaa ctatctgttg tcagtaagat tacccctatg ttgctacaga gacatcaatg
     6121 tttcaacgac cagaagatct tttctgccag accttgcgaa atacccggca cttttgcaat
     6181 ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc atatatttca acaacatttg
     6241 ccgacgtttt ggcccgacgc cttcaatggt ttccagggag ctggtatttt tgaccttcgc
     6301 ccgtttttta cggtgcccgc caatcgcgtg atcgtgtgat tcatcgcgaa tatgctggat
     6361 aacatgcagc gcgggagaat ctggcggcaa actgaatccc tcaccttccg gctcaaagaa
     6421 cagtgtttcc agcccagcct tacgatctac tcctttggca acgccaagta gcagtggatg
     6481 atttttatcc catgagacat ccagttcggc gaagacattt ttcgcctgcg caagctggcc
     6541 tttgccgccg tcgataagga tcacatccgg gatcttactg tcgtcaatgg ctttaccata
     6601 acgccgacgc agcacctgat tcatcgccgc ataatcatcg cccggcgtga tgcctgtaat
     6661 gttatagcgc cgatactccg cacgcagcgg gccgttagca tcaaacacca cacaggaagc
     6721 gacggtttgt tcgcccatgg tatggctgat gtcaaagcac tccatccgct tcacttccgg
     6781 caatttcaac acgctggcaa gcgcggtcag tcgctggtga acggtagatt gctgcgaaag
     6841 tttgctggtt aaggccgtcg ccgcattggt gcgcgcgagt ttcagataac gcgccctatc
     6901 gccgcgaggt ttggtttgaa cattaatctt gcgtcccgcc agttctgaaa gggaatcggc
     6961 gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc tcacccggta aggtgcgcat
     7021 ctggctgcct tgtaaataga actggcctac gaaggtttct accacctcgc tcagttccgt
     7081 accgccaggc actttcggga aatagctgcg gctgccgagc actttgccct gacgaatgaa
     7141 caatacgtgg acacaagcca tgcccgcatc gaacgccaca ccaataacgt cgaggtcgtc
     7201 gccggtattg gaaacgaatt gtttttcggt gacgcgtcgc accgcctgaa tttggtcgcg
     7261 aatacgtgca gcttcttcaa actccagatt ctggctggca gtttccatac gactaatgag
     7321 ttgcgtaagc acctgatcat ctttgccaga caaaaacagg cgcacatact cgacctgctg
     7381 agcgtattct tcttcactca ccagtccttc aacgcacggt cccagacagc gccctatctg
     7441 gtattgcaga cacggacgcg agcgattgcg ataaacacta ttttcgcact ggcgaatggg
     7501 gaaaatcttt tgcagtagcg ccagtgtttc acgtacggca tagccattcg ggaacgggcc
     7561 gaaatattca cctttggcat gcttcgcacc acgatgcatc gccagacgcg ggtgggtatc
     7621 gccactcagg aagataaaag gataagattt atcatcgcgt agcaaaacgt tgtaacgcgg
     7681 ctgatagagt ttgatgtagt tgtgttccag caacagcgct tcggtttctg tatgagtaac
     7741 cgttacatca atttgctgga tctgggcgac cagcgcttcg gttttgcgcg aagcgaggtt
     7801 gctacggaaa tagctggaaa gccgtttttt caggtctttc gctttgccga catagataac
     7861 cgtaccacca gcatcgtaca tgcgataaac gcctggctgg ctggttacgg tttttaaaaa
     7921 cgcttttgcg tcaaactgat cactcactga cttgataatg tctccgcatt acacagacca
     7981 tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa tgtttagttt actgaacata
     8041 cgatagcggt agctgttcac cgttttcgga ctgagattga gctgttctga gatctcattg
     8101 accttctggc ccttggtgat catcagcata atctgcaatt cacgttcaga caaactggca
     8161 aatgggcttt ctgttttttc tggttcgatc tggcttaacg ccatttgttg agcgatgtca
     8221 gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg cactcacgac ttcctgcgga
     8281 gccgcgcctt tgctgaggta gcccgcagca ccggcctgca tgactttcgc tggtaaaggg
     8341 ttttctgtat ggacggtaag catgatgatt ttgacatcag ctgtggaacg cgcgatttta
     8401 cgcgtcgcct caagaccgcc aatgcccggc atactcatgt ccattagcac cacgtcaacg
     8461 gcatttgccc gacaccactt aacggcgtct tcaccgcacg atgcctcacc gacgactttt
     8521 atacccttta tatcttccag aatgcgtcgt atccctgcgc gcaccagttc gtggtcatca
     8581 acaagtagaa cgttgatcaa aggaatatct ccagaaatag ggataacgct actgatagtt
     8641 agtcattcgt atattagcgg tttttattgc aactttgaaa cgttaaaaat ggtgggtatt
     8701 cgatttttct ctcgtttttg tcaattcgat tgtccacagt tatggtcacg cccgtcacca
     8761 acggcttacc ggatcgtttc acagtaagcc ctgcgacgaa atgtgacaaa aattaccttt
     8821 attcagcaaa aatgaaaatc agcccgctga atatgtaaca ttaattaacc cgaacaatac
     8881 gggcaaaaaa cattcatgat aaaaatattt attggtcatt atattaacgc atttaatagc
     8941 accgttgacg atacgctcaa aaaacaacca ctgctatttt tagcaaagct tatggtatac
     9001 tccgccgcct taacattatt caccgcaaat tttcattgca acatgacgag gaaaataaat
     9061 gagtacgcct gatttttcta ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg
     9121 cctgaaagcg atgctgacgc tgatgctgca ggcgatggga caagctgacg cgggccgcgt
     9181 gatgttaaag atggaaaaac agcttgcgct gatcgaagac gaaacccagg ctgcagtatt
     9241 ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag taatataaaa ccggctgata
     9301 gcgtgccttt cagccggttt ttgcatctgg cacgcaggac agaaaagaga tcaaattaag
     9361 ccagtagcgg ccgcgtaaca ggcaacctgg gtcttattcg gtgcattgat ttttttctgc
     9421 atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat cgctatctct
     9481 gctgatgtct tcccttccgc cgtccacctc agaatttctt tttcgcgctt gctgaaattc
     9541 atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc
     9601 accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgcgctg
     9661 caacgggaaa aggacaaaaa gcccagcgcc cggtttggca gcattaaata ctgagtgaca
     9721 ccgcggcgta aaccatgtgc gcgcgcggct tcccataacg gctgtgcttc gttgaataag
     9781 tcatcattcc acattagatg gccctgacta aagttttcag ggttcagcac cggatcaatt
     9841 gcgagaaagt tttttgcctg ataataacta acccacgcct cagggtaatt ggtgtagaaa
     9901 gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac
     9961 tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc
    10021 tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac
    10081 cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaact
//
LOCUS       DAZFKM010000166        10087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFKM010000166 DAZFKM010000000
VERSION     DAZFKM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..775
                     /gene="ruvA"
                     /locus_tag="ACHKAY_004468"
     CDS             164..775
                     /gene="ruvA"
                     /locus_tag="ACHKAY_004468"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO5703024.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            784..1794
                     /gene="ruvB"
                     /locus_tag="ACHKAY_004469"
     CDS             784..1794
                     /gene="ruvB"
                     /locus_tag="ACHKAY_004469"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO5703025.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1941..2726)
                     /gene="znuB"
                     /locus_tag="ACHKAY_004470"
     CDS             complement(1941..2726)
                     /gene="znuB"
                     /locus_tag="ACHKAY_004470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO5703026.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2723..3478)
                     /gene="znuC"
                     /locus_tag="ACHKAY_004471"
     CDS             complement(2723..3478)
                     /gene="znuC"
                     /locus_tag="ACHKAY_004471"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO5703027.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3557..4489
                     /gene="znuA"
                     /locus_tag="ACHKAY_004472"
     CDS             3557..4489
                     /gene="znuA"
                     /locus_tag="ACHKAY_004472"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO5703028.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4505..5827
                     /gene="mepM"
                     /locus_tag="ACHKAY_004473"
     CDS             4505..5827
                     /gene="mepM"
                     /locus_tag="ACHKAY_004473"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO5703029.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQVVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5947..6918
                     /gene="lpxM"
                     /locus_tag="ACHKAY_004474"
                     /gene_synonym="msbB"
     CDS             5947..6918
                     /gene="lpxM"
                     /locus_tag="ACHKAY_004474"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5703030.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7007..10081)
                     /locus_tag="ACHKAY_004475"
     CDS             complement(7007..10081)
                     /locus_tag="ACHKAY_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO5703031.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIDAWITKEPKPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     CNQLLKSLYYDVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDN
                     TLITFSNEEIIKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALTNIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YAFMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRIMENKLHDAEFFITITNNMTSILRSEFDEIWKAML
                     TLQFHDILPGSCISRVYHETEKEYLKLEAKTEKIISDAQSTLLSKIDTSSYKDPHILF
                     NTTCFARNEWININNNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSE
                     SGDLISLYDKRYGKEYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMT
                     TVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQ
                     TSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVK
                     DQTLELTLLRSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVN
                     QAYIMNHPLQIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESYG
                     LEISAMFSWVCNYHYVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2741 a         2311 c         2422 g         2613 t
ORIGIN      
        1 attcaaatat ttgcttccaa tataacctgt agaataaatt atactgcgcc atttttcaga
       61 tcgttgggac gcctcgcaag ttttcttcat ccttcgctgg atatctatcc agcatttttt
      121 tatcatacag cattatcttt gattcattac gcaggagcgt catgtgatag gcagactcag
      181 aggcatcatc attgaaaaac aacccccgct ggtgttaatt gaagtgggcg gcgtaggcta
      241 tgaagtgcat atgccgatga cctgttttta tgaactccct gaagcgggtc aggaagcgat
      301 cgttttcacc cactttgtgg tgcgtgaaga cgcgcaactg ctgtacggtt ttaacaataa
      361 acaagagcgc acattgttca aagagttgat caaaaccaac ggcgtcggcc cgaagttggc
      421 gctggcgatc ctctccggaa tgtcagcgca gcagttcgtt aatgccgttg agcgtgaaga
      481 agtgggggca ctggtgaaac tgccgggtat tggcaaaaaa accgccgaac gcttgattgt
      541 tgaaatgaaa gaccgattta aaggtttgca tggcgatctc tttacgccag ccgccgacct
      601 ggtactcacg tcacctgcca gcccgacgac cgacgatgct gaacaggaag cggttgccgc
      661 gctggtggcg ctgggctata aaccacaaga agcaagccgc atggtgagca aaatcgctcg
      721 ccctgacgcc agcagtgaaa cattaattcg cgaagcccta cgcgccgcgt tatgaggtaa
      781 aggatgattg aagcagaccg tctgatttct gccggtacca ctttgccgga agatgtggca
      841 gatcgcgcca ttcgccctaa attactggaa gagtatgttg gtcagccgca ggttcgttca
      901 cagatggaga ttttcatcaa agcagcgaaa ctgcgcggcg atgccctcga tcatctgttg
      961 attttcggtc ctccggggtt gggtaaaact acgcttgcca acattgtcgc caatgaaatg
     1021 ggcgttaatt tacgcacgac ttctggtccg gtgctggaaa aggcgggcga tctggcagcg
     1081 atgctcacta accttgaacc gcatgacgtg ttgtttattg atgagatcca ccgtctttcg
     1141 ccagtggtgg aagaggtgtt gtatccggca atggaagact accaactgga tatcatgatt
     1201 ggtgaaggtc cggcggcacg ctccattaaa attgatttgc cgccgtttac cctgattggt
     1261 gcaaccacgc gcgcaggttc gctgacatca ccgttgcgcg atcgttttgg tattgtgcag
     1321 cgtctggagt tttatcaggt gccggatctg caatatatcg tcagtcgcag cgcacgcttt
     1381 atggggcttg agatgagtga tgacggcgcg ctggaagttg ctcgtcgagc tcgcggtacg
     1441 ccgcgcattg ccaaccgtct gctgcgtcga gtgcgtgatt tcgccgaagt gaagcacgat
     1501 ggcaccatct cggcagatat cgctgctcag gcgttggata tgttgaatgt cgatgctgaa
     1561 ggtttcgatt atatggaccg caagttattg ctggcggtaa tcgataagtt ctttggtggg
     1621 ccggtaggtc tggataacct tgcggcagcc attggcgaag aacgtgaaac cattgaggat
     1681 gtgctggaac cttatttgat tcagcaaggc tttttgcagc gtacaccgcg tgggcgtatg
     1741 gcgacgacgc gggcgtggaa tcactttggc ataacgccgc cagaaatgcc gtaagtcgga
     1801 ttggtcgctc gcgccgcatc cgacactggc agtgtgtctg atgcgacact gttcgcgtct
     1861 tatcaggcca gccagagata gcgaaccgta ggtcggataa ggcgctcgcg ccgcatccga
     1921 caaatgtgtt cagcgataga ttagctggcc tgctttttca tcatactgag aataaataac
     1981 agtgccgcac atagcaccac cgacggaccc gccggcgtat cgtaaaacgc ggaaaaggtt
     2041 aaaccgccag ttactgccac catccccacc aaaacagcga caccagccat ctgttccggc
     2101 gtacgggcaa agcgacgcgc agtagctgca ggaataatca gcagtgaagt aataatcaac
     2161 gcgccaacaa atttcatcgc tacaccaatc gtcaatgccg tcaccagcat caacaacaat
     2221 ttcacgcgct gtaatttcac accatcaaca aacgccagat ccgggctaat cgtcatcgac
     2281 agcaaattgc gccattgcca gaacaaaata gccaccacga tgaccacgcc aatcgcaata
     2341 gcgatgagat cttctggcgt cactgccagc aaatcaccga acaggtaagc catcaaatca
     2401 acacgaatgt tagacatcag actaacgacc accaggccca gcgacagggc actgtgcgcc
     2461 ataatcccta ataacgtgtc aatcgccagc tgtggacgct tctccagcca taccagaccg
     2521 cccgccagga gcagcgtaac ggcaattacc gcatagaatg gattcacgtc cagcaacaaa
     2581 ccaaacgcga cgccaagtaa tgaggcatga gccagcgtat caccgaaata agacatacga
     2641 cgccagacta caaacgaacc cagcggaccc gcggcacagg cgagcatgat cccggctaac
     2701 caaccgggaa ataataattc aatcatgagc gatcatttcc ccgacgcaaa acaattcgtc
     2761 cctgtaaatc gtgacgatga ttatgatgat ggcgatagat acccagttgt tcagcaccac
     2821 gaggaccaaa catagaaata aactccggat gtagggaaac aacttccggt gtgccggaac
     2881 aacaaatgtg gtgattcaga cacagcactt catcggtttt tgccattacc agatgcagat
     2941 cgtgagagac cattaaaacg ccacaatcca gttcgcgacg caactggtca ataaggtcat
     3001 ataacgccac ctggccattt acatccacgc cttgagtggg ttcatccagc actaataatt
     3061 gcggtcgatt taacaatgct cgcgctaaca gtacacgctg tgtttcgcca ccagagagct
     3121 tttgcatcgg tgcgttaatc agatgcccgg cctggacacg tttcagtgca ggcaaaatat
     3181 cttctttatg tgtgccaggg cgtaagcgta aaaaacggtt tacggtcagt ggcaacgtgg
     3241 tgtcgagata cagcttctgc ggtacatagc cgatgcgtag ttttccgttg cgcttgataa
     3301 ccccttcatc gggtgttacc agcccgagca ctacccgtac cagtgtcgac ttacctgcgc
     3361 catttggccc aagtaaagtc aaaatttttc caggtttaag ttccagcgac acatcagaga
     3421 ggacgcggcg ttggccaaaa gaaaccgaga cattttccag ggaaaccaga cttgtcatgt
     3481 taattttagt cttgcagtag tcatgaaatg ttataatatc acacttctca tattcattac
     3541 gatgattggt cgcattatgt tacataaaaa aacgcttctt ttcgcagcat tatccgccgc
     3601 tctctgggga ggtgcaacac aggccgcaga tgccgccgtt gtcgcttcgc ttaaacccgt
     3661 tgggttcatc gcttctgcca ttgctgatgg tgtaacagaa acggaggttt tactgcctga
     3721 cggcgcttca gaacatgatt attcactgcg tccatcggat gtaaaacgct tacagaacgc
     3781 ggacttagtc gtttgggttg gcccggagat ggaagcgttt atgcaaaaac cggtaagcaa
     3841 attaccagga gcgaagcagg taacgattgc gcagcttgaa gatgtgaaac cgctactgat
     3901 gaaaagtatt cacggcgatg atgatgatca cgaccacgcg gaaaaaagtg acgaagatca
     3961 ccatcacggc gatttcaaca tgcatctttg gctttcccca gagatagcgc gggctacagc
     4021 ggttgcaatc catggaaaat tagtggaact tatgccgcaa agtcgagcca aacttgacgc
     4081 caacctgaag gattttgagg cacaattagc ctcaaccgaa acgcaggttg gtaacgagct
     4141 cgcgccgctc aaggggaaag gttatttcgt ttttcacgat gcttacggct atttcgaaaa
     4201 acagtttgga ctgacaccgc ttggtcattt taccgttaac cctgagattc aacctggcgc
     4261 gcagcgttta catgaaataa gaacacagtt ggttgagcaa aaagcaacct gcgtttttgc
     4321 tgagccacag ttcaggccag cggtcgttga gagcgtcgca cgagggacat ccgttcgtat
     4381 gggaacgttg gatcccctgg ggacgaatat caaactgggt aaaacaagct attcagaatt
     4441 cctgagtcaa ttagccaacc agtatgcgag ctgcctgaaa ggagattaat gaggaagtga
     4501 ttacgtgcaa cagatagccc gctctgtcgc cctggcgttt aataatttac cgcgaccaca
     4561 ccgcgttatg ttggggtcgc tcaccgttct tactctggcc gtcgctgtct ggcggcctta
     4621 tgtttatcac cgtgatgcca cgccaattgt caaaaccatt gagctggaac agaacgaaat
     4681 tcgttcgctc ttacctgaag ccagtgagcc gattgatcaa gctacacaag aagatgaagc
     4741 cattccccag gacgaactgg atgacaaaat cgccggtgaa gcgggcgtgc atgaatatgt
     4801 tgtttccact ggcgatacgc taagcagcat tctcaatcag tatggtattg atatgggtga
     4861 tatcacccaa ctggctgcgg ccgacaaaga attgcgtaac ctgaaaatcg gtcaacaact
     4921 ctcctggaca ttaaccgcgg acggcgaact gcagcgcctc acctgggaag tgtctcgtcg
     4981 tgaaacccga acctatgacc gtactgccgc taacggtttt aaaatgacca gcgaaatgca
     5041 gcaaggagag tgggttaaca atctgctgaa aggtaccgtc ggggggagct ttgttgccag
     5101 cgccagaaac gccggtttaa ccagcgccga agtgagcgca gtgattaaag ccatgcagtg
     5161 gcaaatggat ttccgcaaac tgaaaaaagg cgatgaattt gcggtgttaa tgtctcgaga
     5221 aatgcttgat ggtaaacgtg agcaaagcca gctgctgggc gtacgtttgc gttcagaagg
     5281 taaagattat tacgctatcc gcgctgaaga tggcaaattc tacgatcgta acggtactgg
     5341 tctggcgaaa ggattcttgc gattcccgac ggcgaaacag ttccgtatct cgtctaactt
     5401 taacccgcgt cgaactaatc cggtgacagg tcgcgttgca ccacacagag gtgttgattt
     5461 cgccatgcca caaggtacgc cagtgctttc agtgggtgac ggtgaagtgg tggttgccaa
     5521 acgcagtggc gcagcaggtt attatgtggc tattcgtcat ggtcgcagct acaccacgcg
     5581 ttatatgcac ttgcgcaaga ttctggtgaa accgggacag aaggtgaaac gtggcgaccg
     5641 tatcgcgctt tccggtaata ccggacgttc aactgggccg catctgcact atgaagtatg
     5701 gataaaccag caggtcgtaa acccgctgac ggcaaaactg ccgcgtactg aagggctgac
     5761 cggctccgat cgtcgcgaat tcctggcgca ggccaaagag attgtgccgc agctacggtt
     5821 tgattaatta acatccattc gcagccggta cgcagtcagt accggctttt tttatttggt
     5881 gcggggcaag ttgcgccgct acactatcac cagattgatt tttgccttat ccgaaactgg
     5941 aaaagcatgg aaacgaaaaa aaataatagc gaatacattc ctgagtttga taaatccttt
     6001 cgccacccgc gctactgggg agcatggctg ggtgtagcag cgatggcggg tatcgcttta
     6061 acgccgccaa agttccgtga tcccattctg gcacggctgg gacgttttgc cggacgactg
     6121 ggaaaaagct cacgccgtcg tgcgttaatc aatctgtcgc tctgctttcc agaacgtagt
     6181 gaagctgaac gcgaagcgat tgttgatgag atgtttgcca ccgcgccgca agcgatggca
     6241 atgatggctg agttggcaat acgcggaccg gagaaaattc agccgcgcgt tgactggcaa
     6301 gggctggaga tcatcgaaga gatgcggcgt aataacgaga aagttatctt tctggtgccg
     6361 cacggttggg ccgtcgatat tcctgccatg ctgatggcct cgcaagggca gaaaatggca
     6421 gcgatgttcc ataatcaggg caacccggtt tttgattatg tctggaacac ggtgcgtcgt
     6481 cgctttggcg gtcgtctgca tgcgagaaat gacggtatta aaccattcat ccagtcggta
     6541 cgtcaggggt actggggata ttatttaccc gatcaggatc atggcccaga gcacagcgaa
     6601 tttgtggatt tctttgccac ctataaagcg acgttgcccg cgattggtcg tttgatgaaa
     6661 gtgtgccgtg cgcgcgttgt accgctgttt ccgatttatg atggcaagac gcatcgtctg
     6721 acgattcagg tgcgcccacc gatggatgat ctgttagagg cggatgatca tacgattgcg
     6781 cggcggatga atgaagaagt cgagattttt gttggtccgc gaccagaaca atacacctgg
     6841 atactaaaat tgctgaaaac tcgcaaaccg ggcgaaatcc agccgtataa gcgcaaagat
     6901 ctttatccca tcaaataaaa aacagcctct caaattattg agaggctgta cttgataaaa
     6961 aatctcaatg agtgttctca ccattttgag tttttagatt agtacgctat tgagttaatc
     7021 gtagcgtgat aatttcaaaa ggtttgaatt caagattgca atattgattg caataaaaac
     7081 tgtcatctaa tttattttct tggagatcga catattgaac gtagtgataa ttacataccc
     7141 acgaaaacat ggctgaaatt tctaatccat aactctcata taatcttacg attataccgt
     7201 cgccgtcttc agctaatttt attgtttcga tgataacgct gttagtatcg atagcaaaaa
     7261 atgatagtga agggggtagt tcacccgggt gtggtttgac agggacgatc tgtaagggat
     7321 gattcattat ataggcctgg ttaacgacac cacccacacg gtaatctcca tgatgaggaa
     7381 ataaggagaa tgtgaatttt tgtttaccaa tatctgcaaa attattaatc aggaaattat
     7441 cataattatc tgtcttaaca acttccccag gtttgtgctg cgaacgcaac aaagttaact
     7501 ctagcgtctg gtctttgaca cgatagccat atttgttttt tgctattaaa gcaatgccag
     7561 tttcttgatc agaaaggtcg acccagtgat gtgctggtat ttcatctttg gcaaaagcga
     7621 acgaatcatc gctatgcgtt ggtctgtcaa ttacaccgaa ctgaatttga cattgtgcaa
     7681 gagatgtttg tactgatacc gggaatttta ctttgagaga aacattaggt tcgtgccaat
     7741 caataaaagt ttggacatca acgcgtggac tgtcctgtgt cagggtaaat gcgaagcgaa
     7801 gataagagga attgtagtga taaatcagcg tcatcgtagt tttaggcccg ctgatcacag
     7861 ttgtcatttt ttcagcgagt aaaacatatg attccccatc acgataattt gaagcaaaat
     7921 cccaggccgc gaaaaaacct gcatcttcgt ggtatgcacg gatctcactg tgcatatttt
     7981 cagtaatgta ttctttacca tatcttttat catatagcga aattaaatca ccgctttctg
     8041 aaaaaagaag tttaatgtaa ttattttcaa tacttcgaga ttctgccttc agaccattta
     8101 cgatcttatt ctttggatca atcacagcat aaccataaga gtttactcgt gctttgagcc
     8161 agttgttgtt tatattgatc cattcgttac gagcaaaaca ggtggtatta aataaaatgt
     8221 gcggatcttt ataagatgaa gtgtcaattt tacttaataa agtagactgc gcatcagaaa
     8281 taattttctc ggtttttgct tctagtttaa gatattcctt ttcagtttca tgataaactc
     8341 ttgaaataca tgagccggga aggatgtcat ggaattgcaa ggtcagcatc gctttccata
     8401 tttcatcaaa ctcactgcga agaattgacg tcatattatt agtaatagtt ataaaaaatt
     8461 cagcatcatg gagtttattt tccataattc gattatgggc tttagttgct gactctgatg
     8521 taaagcaccc ttgatgagcc tcgaagtaaa gttcacccga aataatgggc aaggattcgc
     8581 gaaaggcgtc ggcgtgagtg aagaacttat caacacggct gaaatcaaca tgaggtaagc
     8641 catcaatatt gcgtatgcgt gttaatcgtt ctatatgttc ctcaccgggg ccagcgccgc
     8701 catcaccgac accatacacc ataaacgcat agggacagat ttctttttca ttatagtttt
     8761 gttcaatata ttctaaagag cgagcgcggg ctcggctatc atacgtatct tcaggaaagt
     8821 tatgcatcag aatttctgag ccatccaggc cttgccagcg gaaaaggtaa ttattaaatt
     8881 tattaatctt gttttgtgag agcttttgcg atgcaatata gttaatgcct gattttttga
     8941 gaatttgtgg aagcgtggcg gggaatccaa agacatccgg ttgccagaca taatttatct
     9001 tctttgaaaa attctgcagt gtgaatcgtg tcccatgcat gatttgcctt atcagcgatt
     9061 caccacttac cagatttaaa tcgcattcga cccaaaagca accttgtaac tcaaagcggc
     9121 ctgcggcgat ttgttttttt aacttttcga agaaataggg ataatctttt tttatccagt
     9181 gaaaaagttg gtactgacta gcgccaaaaa tgtaatcagg atatttttca atgtttgtca
     9241 gggcggtggc aaatgttctt attgcttttc tgcgtccttc tcttaaaggc cacatccacg
     9301 ctatatctaa atgggcatga ccagtagcca taatcctgaa ctctgaagga ttattgtttt
     9361 tactaattat ggattggatt gcaatttgac atttaattat ttcttcattg ctaaaggtaa
     9421 ttaacgtatt atcaatatat tcgagaattc ttatgatttc gtcagtacgc tcgcttcttt
     9481 tggtgataat tttttctggg ataatacctt taggatgtat ggattcaaac ttctgaccat
     9541 cattaatcca gtcgaaaagc acttcaacat cataataaag ggatttcagt agttgattac
     9601 atgtcgcgat atgaacatca gtaataatcc ctccattagt gacatagcca aataaatcgt
     9661 tgcacgcagc atcaatccaa aactcgacct cgtcattttc actaacgaga gaaaggtcaa
     9721 ccacccattt acctggttta tctggcggta cgccataaga tgatgcttta ttcgtgatgc
     9781 cgcagatggg tgaaccattc ttatccacaa taagaccttc gccaccgata tcaacgagag
     9841 cgacgagttt gtgtctaaat ttatctgctg gacgtttacc ataaactcgc atccaggcac
     9901 agtcaaacag ttgaccccag tctgagccaa tggtgagttt ctggtaagcg ccgctacggc
     9961 gataaacaaa aggcttcggt tcttttgtta tccatgcatc aatcgacatt tcagagacaa
    10021 cagtataaac acgctgacct attttattcg acagtttgat aatattatca gtaaggtaca
    10081 tagaaat
//
LOCUS       DAZFKM010000167         9929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFKM010000167 DAZFKM010000000
VERSION     DAZFKM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..577)
                     /locus_tag="ACHKAY_004476"
     CDS             complement(<1..577)
                     /locus_tag="ACHKAY_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO5703032.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKISLII"
     gene            complement(574..2262)
                     /gene="yfaA"
                     /locus_tag="ACHKAY_004477"
     CDS             complement(574..2262)
                     /gene="yfaA"
                     /locus_tag="ACHKAY_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO5703033.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2411..5038)
                     /gene="gyrA"
                     /locus_tag="ACHKAY_004478"
     CDS             complement(2411..5038)
                     /gene="gyrA"
                     /locus_tag="ACHKAY_004478"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO5703034.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5185..5907
                     /gene="ubiG"
                     /locus_tag="ACHKAY_004479"
     CDS             5185..5907
                     /gene="ubiG"
                     /locus_tag="ACHKAY_004479"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO5703035.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(6035..9769)
                     /gene="yfaL"
                     /locus_tag="ACHKAY_004480"
     CDS             complement(6035..9769)
                     /gene="yfaL"
                     /locus_tag="ACHKAY_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO5703036.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEDYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2321 a         2743 c         2390 g         2475 t
ORIGIN      
        1 tgataattaa tgaaatttta atatacaact tccatgtcat aagttgttgc agactgactg
       61 cgcgcattcc gttgtcagtt ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca
      121 tccagaccat taagtgctgg gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg
      181 cctggtttat gtcctggcca ataaactggc tgttgtactg aatcaaatta atcgcggtca
      241 cgtaggggcc ggttttcccg ttcccctgat tccagttttg cgccagttga cgctgtccag
      301 gtgttagcgt catctctggc ggcaaatact ggctagagaa accgttactt tttagccatt
      361 tactatcgtg aactttcagc gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct
      421 gctgatacca gcgtggactt ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc
      481 aggcgcgaaa cagcccggat tgttcgacat tcagcatttc gctatgggca acaacacaac
      541 acagccagca aatcagcgcc agcagcccgt gcctcatagc ggctgccagg ttatgggtag
      601 ccactgccag gcggcaccgg gttccatctg ggccagcttc atgacataac gcggttgttg
      661 agataaagcg tccagcttcg gcattaataa agtttgtgcg gcgttataaa aaaccggttc
      721 gagattcttc ggcagactgg tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg
      781 attgatatag agcggaacga tgccatcagt gggtattaca tccaccattg ccgggcggtt
      841 tttattcagt gtttgcaggg cgttattaac cagcgtgtca tcgagggaga aaagcagcgt
      901 tttgttttgc atcgccagcg acacgcggaa aaaataatcc gacattaatt gatcgggttg
      961 cgccgcctgc gctttcggat actgaccgta tcgggaactc acttcgcgac gccagatttg
     1021 cgcttcgccc tgctgagtct ggcttatcgg caaaacacct tctggcgctt tgctttcgtg
     1081 cgcaccaata ttttgcgtaa acagtttccc tggcaattgc gcctgttcgg cagtgccatc
     1141 aaactgaccg acaaacagcg gggtttgcaa ttttgagtct gcataccagc acagccccgc
     1201 ggcaccgtct aacgccccat tcaacttgtc gttttcctga ctgatgtgcg aaagcatctc
     1261 ttcggcaata ccgtgtgaat acggcaccac cacacagaag ctggccccgg caggcatact
     1321 gttccagacc ggcgtaaaat cgaaactggt atctacgctg gcggattcat catttaacgc
     1381 cacaaaactg tgccagccgt cgttacccat ttcgaagcgt acgccagcaa aagaaggcat
     1441 taaccgctgg tagccaaaac ccagcaacct ggcgctgacc acgatgcgct ggcgtactgg
     1501 cgttttttca gcagtacgct cttccaggcc aaagcttgct tgccagcgtt ttttgccgct
     1561 caacaaatca cctgcgatcg ccgtggcttc ggtatcctgc tgatcatctt taaacaacat
     1621 atccgtgctg gaaaacacca gcatcttgtc ctgataagtc gcgaacatca gggcgttatt
     1681 gccgttatag cgcaactgat aaacaggaac agtttcacta tttatcttga tgctactgat
     1741 ttccgttttg cttaactggc tgtcgctggt agcggcaaac aacaatggct ccagcagttt
     1801 gcttaaacca ctgcgctgga tcagcaccat ataatgtgaa agatggcctt gtttatcgtg
     1861 ccacagcgct gcctgcgcgg gctgatctaa gagtgacgaa aagagcttat ctttcagcgt
     1921 gagatcgtgt tcatagacaa tacgacgaat gctgccttca atgcccagac gatcggcatg
     1981 attctgataa tagaaaacga aatcttcgct cagaacatcg tggagaaacg gaatggtgag
     2041 gagatctttg ggaagctggc tcagagagtc gctgtcgaga aagaggtccg gctcattgag
     2101 atcgatttgc agattgttgt gcaccaccag cggcgacaac gttttttctg gcccactgcc
     2161 agcatattgc aacgcccaga cgccagcgga aagcagtgct attgcgccaa aacctacaag
     2221 accatagaac cgccagcctt tcgccttttt ttcaccactc attgccacat tccttgtgta
     2281 tagccagcca ttttttacgg gcacagccaa actttaccgt gccctaatac gacaaaagcc
     2341 cagactttgc agcctggact tttcaattca aacaagggag atagctccct tttggcatga
     2401 agaagtaaaa ttattcttct tctggctcgt cgtcaacgtc cacttccgga gcgatttcat
     2461 cgtccccttc cgcggcactg ccatcgatgg tatccagatc ttcctcgtca accggttcag
     2521 caacacgttg cagacccact acgttttcat cttccgcagt acggatgagg atcacgccct
     2581 gggtgttacg gcctacgatg ctgatttccg aaacgcgagt acgtaccagc gtaccggcat
     2641 cggtgatcat catgatctgg tcgcagtcat ctacctgtac cgcgccaacc actaaaccat
     2701 tacgttcagt aaccttaatg gagataaccc ctttcgtcgc acgcgacttg gttgggtatt
     2761 ccgccactgc ggtacgttta ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc
     2821 cacgaggcac gatcagagag acgactttat cgccttcacc taagcgaata ccgcgaacac
     2881 cggtggtgtt gcagcccatc gcacggacag aagactcttt aaagcgcacc actttacctt
     2941 cagcggagaa cagcattact tcgtcttcgc cgctggtcag gtcaacgccg atcagctcat
     3001 cgccgtcaac cagtttgatc gccactttac cggcggtacg cagacggttg aactcggtga
     3061 ggacagtttt cttcacggta ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa
     3121 actcggtcac tggcaggatc gcagtgatac gttcgtcctg ctccagcggc agcaggttga
     3181 cgatcggacg accgcgcgcg ccacgagtgg cttccggcaa ctgataaact ttcatcgaat
     3241 agacgcgacc acggctggag aagcacagaa tatggtcgtg agtgttcgcc accagcagtc
     3301 ggtcgataaa gtcttcttct ttaatacgtg cggcagattt acctttcccg ccacgacgct
     3361 gcgcttcgta ttcagaaagc ggctgatact taacgtagcc ctggtgagag agcgtcacta
     3421 ccacatcttc ctgagtgatc agatcttcca ggttgatgtc tgcgctgttg gcggtgattt
     3481 cagtacgacg tttgtcaccg aactgttcac gaaccagctc cagctcttca cggatcactt
     3541 ccatcaaacg atcggcgcta ccaagaatac gcaacagttc cgcgatctga tccagcagct
     3601 ctttgtattc gtcgagcagt ttttcgtgct caaggccggt cagtttctgc aaacgcagat
     3661 ccagaatcgc ctgagcttgc tgttcggtca ggtagtacag accatcacgc acgccgaact
     3721 ctggctccag ccattccgga cgcgcagcat cgtcgccagc acgttcgagc atcgcggcaa
     3781 cgttgcccag ctgccacgga ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg
     3841 catgacggat cagttcgatg atcgggtcga tgttcgccag cgccacggct aatgcttcaa
     3901 ggatatgagc acgatcgcga gctttacgca gttcgaaaat agtacgacgg gtcaccactt
     3961 cacggcggtg acgaacaaac gccgcgatga tgtctttcag gttcatgatc ttcggctgac
     4021 catggtgcaa tgccaccatg ttgataccga aagaaacctg caactgggtc tgggagtaga
     4081 ggttgttgag cacaacttca ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac
     4141 cgtctttgtc agactcgtca cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca
     4201 gttccgcaat cttctcgatc aggcgcgctt tgtttacctg atacggaatt tcgtggacga
     4261 taatggtttc acgaccggtt ttggcgtcaa cttccacttc tgcgcgagcg cggatataca
     4321 ccttgccgcg accggtacgg taagcttctt caataccgcg acgaccgtta atgattgccg
     4381 ccgtcgggaa gtccggcccc gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat
     4441 catcgatata cgccagacaa ccgttgatga cttccgtcag gttgtgcggc gggatgttgg
     4501 ttgccatacc tacggcgata ccggaagaac cgttcaccag caggttagga attttggttg
     4561 gcatgacgtc gggaattttt tccgtgccgt catagttatc aacgaaatcg accgtctctt
     4621 tttcgagatc ggccatcagt tcatgggcaa ttttcgccag acggatttcc gtataacgca
     4681 ttgccgccgc agagtcgccg tcgatggaac cgaagttacc ctgaccgtct accagcatgt
     4741 aacgcagcga gaatggctgc gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat
     4801 ggggatggta tttaccgatt acgtcaccaa cgacacgggc agatttttta taggctttgt
     4861 tccagtcatt gcctagtacg ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc
     4921 catctcggac atctggcagc gcacggccaa caatgaccga catcgcataa tccagatagg
     4981 agctcttcag ctcttcctca atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct
     5041 aaccgctatc cctctactgt atcccggatt caaaggtcgc aaattataac acagccgcgc
     5101 agtttgaggt aaacctatac gctttattca catccaatgc ctgatatact cgtttgtctt
     5161 gccaattacg gagtagaagt gccaatgaat gccgaaaaat cgccggaaaa ccataacgta
     5221 gaccacgaag agatcgctaa atttgaagcc gtcgcctccc gctggtggga tcttgaaggt
     5281 gagttcaaac cgttgcaccg cattaacccg ctgcgtctgg gctatattgc cgagcgtgct
     5341 ggcggtttat ttggcaaaaa ggtgctcgat gtcggttgtg gcggcggcat tctggccgag
     5401 agtatggcgc gcgaaggcgc gacggtgacc ggtctggata tgggctttga gccattgcag
     5461 gtggcaaaac tgcacgcgct ggaaagcggc attcaggtgg attatgtgca ggaaaccgtg
     5521 gaagagcacg cggcaaaaca tgccgggcag tatgatgtgg tgacctgcat ggagatgctg
     5581 gagcacgtcc ccgatccgca gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc
     5641 ggcgatgtct ttttctcgac gcttaaccgc aacggcaagt catggctgat ggcggtggtt
     5701 ggcgcggaat atattttgcg catggtgccc aaaggcacgc atgatgtgaa gaagtttatt
     5761 aaaccggcag aactgcttgg ctgggtagat cagacaagcc ttaaagagcg acatataacc
     5821 gggctacatt acaacccgct cactaatact tttaaactcg gccccggcgt ggatgtgaac
     5881 tatatgctgc acacgcagaa taagtgaggt tgttgtttgg ccgagccaat gcctgatgcg
     5941 acgcttgccg cgtcttatca ggcctacaaa tgctccccgt aggccggata aggcgtttac
     6001 gccgcatccg gcaaccgtgc cgactagaca gtgattacca tttcaccgtc atcgacaaaa
     6061 accctgccgt ctgggcaaaa tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg
     6121 acactcgctg actgatattg cccgtgactc ccacttttat ttcaccccgt cgcttcaccg
     6181 catcgtcact gatagtgctg ccatcttctt caatttccgt cgcatgggga tcgtgataat
     6241 aattcagatc taatgttggt gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca
     6301 gccgcgtctg aatatcatcc ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa
     6361 aatcatcctg ctgcacgccc tgataaatca cctgcgcctg cggttcaatc accacaccac
     6421 gccccggtaa ccactgatac ccggcttcca gcgaggcgat aatccccgac gagtggtaat
     6481 gatctgtgcc atcgtcttgt tcggaaacat cattgttaaa ccacgcgtat tgcagccagc
     6541 tatccagcca ggccccctgc ttctgattac cgtgctgata ccagcttgag gtcagcccaa
     6601 cggcataacc gtggttctgg ttatcggcgc gagttccggt catattcgag cggctctcgc
     6661 cctgattatc actgtagcca ccaaccgccc caagcatcca ctcgccatca tcgccccagc
     6721 gcccgctaaa caggtcaccg ctaagctgca ccgtagaagt gtcttcatgc tgagccagtt
     6781 gccctgccgc cgtgtaatga taacgtccgc cgataacacg taaattcagc gtctgaccat
     6841 tgccacctgc atgatcgcgt cgctccatca taaacgcctg atttgccgcc cgcagattat
     6901 tgagataacc gccaactttg gcattcagca ccggctggta agcggggatt ggatcgggtg
     6961 ttgggtcagg atcgggtgtt gggtcagggt ctggatcagg tggcaatggt ggcgttactt
     7021 cttgcgatcg cagataccag tcgttgttat cttccaccag cgtgtagtca tatgctccca
     7081 tattgacgta gccgctgcct gccagactga acttcgcatt gttttgaaac tgcgtgggat
     7141 cggctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     7201 agttaaccac cacagttgtg ttgccagcag tattaccgtt caacaccaat tgatcgctta
     7261 ccgagtcatc gccgtttaat tcgctatcga gcagtagcgt accgccgccg gtataatcac
     7321 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     7381 tggtcagatt gccattaacc gtcacgtcgg aaagataggt caggctgtca gtttcaaggt
     7441 tggtattaag ctcacctgtc aacgtcacgc catcggtggc attcaccagc gtcccatcgc
     7501 cgttaaacag tgaggcatta agggcgacag aagtatcctg cccggtcagg cgcaaaaccg
     7561 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aatagattga atatcctgat
     7621 ccgcgccagt aacgaacgtc gccccatcac cgacccacag tgacgaagca taaggaagga
     7681 tatccgcgac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
     7741 cgctggcggt cagctccagc gtacccgccc ccgttttagt caatgtgcta ccctcatcct
     7801 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
     7861 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
     7921 gagtatcgac attggcagta atctccatca cgctttcttg ttgcttatcg gtaaagataa
     7981 cctggcggtt atagtgggta tctccaagct gtgagttgtc gctcacctcc agcataccgg
     8041 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
     8101 ccgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc atatcgtttc
     8161 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccgctgactt
     8221 caaggccatc gttgtatgat gtgccactct gccaggtgga gaaatcagag agatcgagca
     8281 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
     8341 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
     8401 actgcgcccc cgacagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
     8461 cgatggtgcc agcaaaactg ccttgattaa cagtaacatt gccaccagca tcgatattta
     8521 aagtgccatt ctgaaagccc gtcaatgagt gcacaaaggt ttgttgggtg gagccaacgt
     8581 ttagttctgc ctgattctgg tacttatcaa tactccctat cgtcagaccg tagcagtctt
     8641 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
     8701 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
     8761 ccagatcgcc ggaacctgtt ttggtgatca acccggtacc agcaatagag tcaacagctc
     8821 cgtcattctc tgtattgcca ataaccagcg tttttccgtc ggcaatatca aaggtaactt
     8881 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
     8941 ctgcgctgtt gttctcatcc actaacacgc cgccgttctg gctgtagctg tcatcaacag
     9001 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataatctt
     9061 cggcagtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
     9121 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
     9181 atgtatatgc ctggttgtta tcaaaaataa catcacttaa ataaacgtca ttattaatgg
     9241 tataaattgc gccacctttg ccatcattag cgatgttatt gcgaaacatg gcgttagtga
     9301 cacgtaaatc gacgacacca gtatcattag taccagaaga atagattgcg ccaccatagc
     9361 cgcctgcgac gttaccagaa aaaataacat cagtaagatt cagcgttgag ttttctttag
     9421 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
     9481 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggctc
     9541 ctccgctggc gttattggtc atatccgaaa aaaccagcca ttgcccatcg gcgacactcc
     9601 agtcctgcgt aatgcctgaa aggctttgcc tgctggcgtg acaactcgct ttgatatcat
     9661 atccctggca taaatcaatg gccgccgcga aaccgttagc agagaaaaga gatgcaatca
     9721 ttgacgggag tagagataaa tactccttgc gtagaaagat aatccgcata ttcatctaac
     9781 catcatttta tataagacgg catatttaaa cgcattatac aatatgggtt atctttcttt
     9841 tgtaaattgt ccaacccgat gcaatggcta ttttgttatt atttgatgaa tagtatcagt
     9901 gcgtcataat tcaagttaat aaccttaag
//
LOCUS       DAZFKM010000168         9895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFKM010000168 DAZFKM010000000
VERSION     DAZFKM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1373
                     /locus_tag="ACHKAY_004481"
     CDS             <1..1373
                     /locus_tag="ACHKAY_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703037.1"
                     /translation="NLETVWMQISSTLKGALDINSLIIDYDNEDVQSYWEDVVLPNLN
                     DNGNKALTRDEIIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIAEYAVSRNAI
                     ISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLNDSLIEWKPS
                     YYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRCRNIDFVFGNL
                     SEELYQRLKNNNTLDEFIKSVFDSYSNEYANSGVAALLQYCSSKMDLPSNNDTYHEMY
                     HALNMNLSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHR
                     LIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLNHGT
                     TVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDY
                     VSMMLP"
     gene            complement(1445..1861)
                     /locus_tag="ACHKAY_004482"
     CDS             complement(1445..1861)
                     /locus_tag="ACHKAY_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703038.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(1900..3129)
                     /locus_tag="ACHKAY_004483"
     CDS             complement(1900..3129)
                     /locus_tag="ACHKAY_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO5703039.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(3428..4336)
                     /locus_tag="ACHKAY_004484"
     CDS             complement(3428..4336)
                     /locus_tag="ACHKAY_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFO5703040.1"
                     /translation="MARTLVNVSATIFALMMIVRALFTYIYPGKLPFSIAIIDWLIVI
                     AGSGAAISSIFCFIKKRYPDTAEFLPMFSTVCYVIILIGYAILRYTPAYQTSLSIMVT
                     GMLVGMGWWIQCITSAANTRRSHTLNMIINTRTSPEYQKQLRNSTKFYRGMRYVPQEL
                     SEWRCNPDKEEYKNMKVPDEYRDAINGLLYILNYFEFLAQGIKFKDLDDELLRECFSS
                     FLRGIERRGFHMILESQKQDPAAFEGIIYLSKKWNGTSFVETHRSNPNTVELGVPYPS
                     NDMVEKMVQGQPLIDSDAGPELQVAT"
     gene            complement(4819..5994)
                     /locus_tag="ACHKAY_004485"
     CDS             complement(4819..5994)
                     /locus_tag="ACHKAY_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO5703041.1"
                     /translation="MGKKEQGLLSLPRGVTIRKHKTASTLVITFTYKGVLCREPLSRL
                     EANTRGIKYAERLLGEIQNQIASGTFEYAKYFPNSKKLELFGVVKKTKNIKSYLDEYL
                     KICINRNLSPSTIGGYEKCLSALSELHKLHVTELTPAILKNWIASRKTKLKTIRNNLS
                     FLRSAIDEAVTDGLLTINPVTLVSASRYHVIDSSPSADDYEVDPFTPAETLAIYQSCR
                     YREWENLFRFAFNTGLRSSELCALRWADLDTIANTAHVQAASVVGVLKGTKTKAGTRK
                     VELNSEALAALQAQKQYTFMKSEFIFSDPKTGEPWTNADAIRKKAWVPTLKKAGVRYR
                     NPYQTRHTFATRHISQGVNLFWLAGQMGHKGPEMLFRNYGKYLAEYDGKTSINVVSS"
     gene            complement(5949..6158)
                     /locus_tag="ACHKAY_004486"
     CDS             complement(5949..6158)
                     /locus_tag="ACHKAY_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO5703042.1"
                     /translation="MGLDCVPISTYCRNAGETVDAVNKRIQRGIWKEGVHVLKVDGVK
                     ERWVDLIEVSKWARKNKDYYLCQEE"
     gene            complement(6167..6313)
                     /locus_tag="ACHKAY_004487"
     CDS             complement(6167..6313)
                     /locus_tag="ACHKAY_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703043.1"
                     /translation="MHTVFELWVRKTFGNRYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LNVV"
     gene            complement(6317..6559)
                     /locus_tag="ACHKAY_004488"
     CDS             complement(6317..6559)
                     /locus_tag="ACHKAY_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5703044.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRCTDRSVFFRRMDGHSGRVCIG
                     EFNCLFEHIDHQQYCKILADTEQEKHLKKLRAMQRR"
     gene            complement(6644..6808)
                     /locus_tag="ACHKAY_004489"
     CDS             complement(6644..6808)
                     /locus_tag="ACHKAY_004489"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5703045.1"
                     /translation="MPNDAQWCVVNTEYGYYVQCWSEGQGWLGDDISIPECDVTHWMP
                     LPEPPQEVSQ"
     gene            complement(6896..7159)
                     /locus_tag="ACHKAY_004490"
                     /pseudo
     CDS             complement(6896..7159)
                     /locus_tag="ACHKAY_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(7382..7507)
                     /locus_tag="ACHKAY_004491"
                     /pseudo
     CDS             complement(7382..7507)
                     /locus_tag="ACHKAY_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            complement(7509..7838)
                     /locus_tag="ACHKAY_004492"
     CDS             complement(7509..7838)
                     /locus_tag="ACHKAY_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5703046.1"
                     /translation="MSKIDYQALREAAEAIKVVATPQKLLAFRMKVTPQVVLVLLDEL
                     EAKNKRITELEAREVQLPTRYDLRYGHPINADKRHVMIPKENGSWLCLIDLEHALRVA
                     GIRIKGE"
     gene            complement(7835..8074)
                     /locus_tag="ACHKAY_004493"
     CDS             complement(7835..8074)
                     /locus_tag="ACHKAY_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703047.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTREAFFSRIAAQIRKGGNQ"
     gene            complement(8067..8270)
                     /locus_tag="ACHKAY_004494"
     CDS             complement(8067..8270)
                     /locus_tag="ACHKAY_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703048.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(8267..9145)
                     /locus_tag="ACHKAY_004495"
     CDS             complement(8267..9145)
                     /locus_tag="ACHKAY_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFO5703049.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVLKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWQKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(9136..9672)
                     /gene="yfdR"
                     /locus_tag="ACHKAY_004496"
     CDS             complement(9136..9672)
                     /gene="yfdR"
                     /locus_tag="ACHKAY_004496"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO5703050.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(9801..>9895)
                     /locus_tag="ACHKAY_004497"
     CDS             complement(9801..>9895)
                     /locus_tag="ACHKAY_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703051.1"
                     /translation="QEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT         2605 a         2229 c         2114 g         2947 t
ORIGIN      
        1 agaatttaga aacggtttgg atgcagatat catcgactct caaaggtgct ctggatatca
       61 acagcttgat cattgactac gataatgaag atgtccaaag ttattgggaa gacgttgtat
      121 taccgaactt aaatgataat ggtaataaag ctctcacaag agatgaaata atagcctata
      181 ttaaagacag taagccttgt agaagaatta aaagattaat acatgatgat gctgtgttgg
      241 aggatcaaaa ggataaaaac ttcattgcaa aaatgattgc agaatatgct gtgtctagaa
      301 atgcaattat ttctagcaac acatctggca atgtcattcg gggtgatgaa agcggagtaa
      361 aatggattca atgtggtaat atctttgtct ccctatttca taaggttcaa gatgatcatg
      421 aaaacgatgg agataggatt tggcaaactc tcaatgattc tctcattgaa tggaaaccat
      481 cttattatca gttaataaaa tctgaaattc agaatgcaat cgaagctgag gctttatctt
      541 ttgtaaatca tttggctaac gatcattacg gtcaagctgc gtggttaaat gagatattaa
      601 aatcagactc gcctgatatt agatgtagaa atattgactt tgtatttggt aatttatcag
      661 aagagcttta tcaaagactt aaaaataata atacgctgga tgaatttatc aaaagtgttt
      721 ttgatagcta ttcaaatgaa tacgctaaca gcggagttgc tgcattgctc caatattgct
      781 cttcaaaaat ggatctgcca tcaaataatg atacttatca cgaaatgtat catgctttaa
      841 atatgaattt gtcttcaaag aattttgaag atggtcatat ttctactggc actattttct
      901 ttgatacaga gtcgaacaaa tggtatttat gtgtatctgc ggcatgtgat ttggttccta
      961 ctcagggtaa cgaccctcac catgtaagat taagtccgca caggctcatt aaagttctgg
     1021 agctttttaa cgccagtcag agtaaagcat tgccatttgc tgaacattcg aaatatatat
     1081 atgtaatgca taaaaatcaa agaaaatatc tctctatttt cgaaggggat aaaacgcttc
     1141 ctgttgttga ttatatggtg gtgttgaatc atggaacaac agttgatggc gaagaaaaaa
     1201 atattatttc tgccgtgttt ttaagtaata tggatggcaa cgtgcaaaat gttcctgtcc
     1261 gactcaaact taaatctcaa ctgagaactg gttatgcaga aagatatcag gctatagcgt
     1321 ctcagtatag ctcaaggatt ggtgtggatt atgtatcaat gatgctacca taattattat
     1381 attttaggcg tggtgatttt ttttcgccat gcctatttta tatttatcat cacaatgatg
     1441 ttgtttattt ttgtttaagt cttctaagct tcatgcattc taatgagaat aataatagag
     1501 ttgtgctgta tataaagcca gagttagtta ataactttaa atttaatgct tcaggatttt
     1561 cactgagaaa gtgaaaggca taaactaagt atacagctaa agtagccgtt ccaattgttg
     1621 gtccaatatc gccttgatca tgaggttcaa ggtttacatt gaacttgaag attagccatt
     1681 caaatcccca agtaaaaaaa acaactaata tcatggctgc gaataatttc gcgatgttat
     1741 ctgctgatgg attcatcaga acagattgtt tctgaaaaaa ttcacaaagt gagagtccga
     1801 aaaaaataaa aaaaagtggt ctggaactaa atttttcaaa aactttgaat agggtttcca
     1861 ttttctttac cttcaggatt atattagcta ttctgatact taggctacca gagcatttga
     1921 tctttgagct tgaacgtaat cactccacca ttgcatcaaa ctctgtcgct ctatcagata
     1981 ttctgcacga ttgtatgctg cgataatttc atcttttttc gagtgggcaa gcgctgcctc
     2041 aagaacttca gctctgaatt taccagactc ctctgccgct gttcgtgcaa tagaacgcat
     2101 accgtgagct acaagctcgc ctccgaaccc cattcggatg atagctgcgt tggctgtttg
     2161 ttcatgcata tgattaagag gcgcttttat gctggggaaa acccattctc tatgcccact
     2221 tattgatttc attaattcaa ggatgcgcaa agcttcttta ctcaaaggaa ctttgtgaag
     2281 ctttttcatt ttcatgaaat cagcaggaat gttccaaatg ctgttggttg tatcaatatc
     2341 agaccacctt gcgcgaacgg cttcacccgg acgaacccat gtcaacaatt gccattcaat
     2401 tagcatacgt gtttccaacc ggattgacgc attcgtcaaa gattccataa accttggcaa
     2461 ttcgcttggg ggaagggcag gcatattttg cttttttggt ttactgaatc tttgaccaag
     2521 gttgtcagcc gggttgaact caataagttc ttcagtagct gcccaccgga agatttcatt
     2581 cagacgtgaa atgatacggc gtagagtttc caatacgcct cgttgctcaa taggatcaag
     2641 gtgttgtttt aagagcttag gtcggatctc attgataggg acattaccca gaccgggaaa
     2701 gacatttctc tctaagctgc gccagatgtc tgctgcatgg tcttgtgaga tacctgatgt
     2761 ctttaccttc tcatctaacc atttccgcgc tacggcttgg agagtgtgct cagtagcact
     2821 ctttaatgcc ttcgccttat cgttgttatg gatttgggga tcaacaccat ttgccagaaa
     2881 ggagagatat tcatcacgta aggctctggc tcttgcaagg gtaaggtgag gatatgtccc
     2941 aaggctcatt ttggttcttt tcttgctcac gggtactgca tacctgaaat accaattttt
     3001 cttgcctcct ttcgccaaag gagcgattcg tagaatcaga ccatcaccgt caaacaagtt
     3061 gatttcttta tcggctggct tggtgctttt gatttcagtg tcagtgagct tcttagcgat
     3121 ttttgccatt ttgggaccct cggtttttgg acccttctta gtgggtccca ttcagggtgc
     3181 cataactcgt agttctcagc aattctcact ggacgacaat agacgtaaaa aagcccgcag
     3241 agcttgtgct gtgtgggctt agtagacttc attgaacttc aaacaactaa aaagtggagc
     3301 tggcgggagt tgaacccgtg cccgagcaat gcttaaccca ttgaataaca atgcattctc
     3361 tgtttaaaaa tgcctcaagt gcattttgag tgcattttct ggtcctctct tggtccatac
     3421 aacgagatta agtggccact tgaagttccg gccctgcgtc gctatctatt aacggctgac
     3481 cttgaaccat tttttcaacc atatcgtttg aaggatatgg aaccccgagc tcaaccgtat
     3541 tagggttgga acggtgggtt tcaacaaacg atgttccgtt ccattttttt gaaaggtaga
     3601 ttattccttc aaaggctgca gggtcctgtt tctgagactc taagatcatg tggaagccac
     3661 gacgttctat cccgcgtaag aagctggaga agcactccct cagtaattcg tcatccaaat
     3721 ccttgaactt gataccctgc gcaagaaact cgaaataatt aagaatatat aaaaggccat
     3781 tgatggcatc cctgtactca tcggggacct tcatattctt gtattcttct ttgtctgggt
     3841 tgcagcgcca ctctgatagt tcttgtggta cataacgcat gccccgatag aatttggtgc
     3901 tgttccgaag ctgtttttgg tactccgggc ttgttcgggt gttaatgatc atgttcagcg
     3961 tgtgggacct gcgcgtgtta gcagcggacg ttatgcattg tatccaccat cccattccaa
     4021 cgagcattcc tgtaaccatg atcgaaagcg atgtttgata ggccggtgtg taccttaaaa
     4081 ttgcatatcc aataaggata atcacataac aaacagtgct gaacatagga agaaattctg
     4141 cagtgtctgg atagcgtttt ttgatgaagc agaagataga agatattgct gcaccagaac
     4201 cagcaatgac gatcagccaa tcaatgatgg cgatgctaaa tggtagtttt ccagggtaga
     4261 tataagtaaa aagtgctctg acaatcatca ttagagcaaa aattgtagcg ctgacgttga
     4321 ctaatgttct cgccatctta ttgattccta taacaaaaga cctccgcatg ggaggcctta
     4381 ttgtgctaat ggaggtttgt tttaaaattt taggttgtag ttaaccttca ccgaaaccta
     4441 cggtcagtgt ctttttcata gtgttcactc cagttgtggt gcttcgccga tacctacgtt
     4501 aagctcttga aaagtaaaaa aacataaaag ttttagaatt tgtggagcac aggataacag
     4561 tttgtactgg gactcatcaa gtactatcag tgtccttgca ctgtcctagt gaaaatttat
     4621 tgcaataaag taacttacgt gaagtcaaga aatatatttg cactccattt cttgctatga
     4681 tgtaagttat tgattttaat taaatttaca attacctaaa tgaactatgg aaactatcaa
     4741 taacatagat gatagctcat ttcacaaaaa gcaatggtag actcattctt aattttcatc
     4801 agccttaagc ctatgtactc agcttgacac aacatttata gacgttttac catcgtattc
     4861 agccaggtat ttaccgtaat tgcggaacag catttccggc cccttatgcc ccatctgccc
     4921 ggcaagccag aaaaggttta caccctggct aatgtgtctg gtggcgaatg tgtggcgcgt
     4981 ctggtacggg ttacggtagc gcacaccagc ttttttcagg gtcggcaccc atgctttttt
     5041 acggatagcg tcggcgttcg tccagggttc tcccgttttc ggatcgctga atatgaactc
     5101 acttttcata aaggtgtatt gcttctgcgc ctgcagggct gccagcgcct cactgttcag
     5161 ctccacctta cgggtaccgg cttttgtctt ggtgccttta agtaccccta cgacactggc
     5221 cgcctgaacg tgggctgtgt tcgcgatggt gtcgagatca gcccagcgca gcgcgcacag
     5281 ttctgagctc cgcagacctg tattgaaagc aaagcggaac aggttttccc attccctgta
     5341 cctgcagctc tggtaaatgg caagggtctc cgctggcgtg aacgggtcaa cctcgtaatc
     5401 gtcggcgctc gggctactgt cgatcacgtg gtaccggctg gcgctgacga gggttaccgg
     5461 gttaatggtc agcaggccat ccgtaacagc ttcatcaatg gcgctgcgca gaaatgacag
     5521 gttattcctg attgttttca gcttagtttt tcggctggct atccaatttt ttagtatcgc
     5581 aggcgtcaat tctgttacat gtagtttatg tagttctgat agtgctgata gacatttttc
     5641 gtaaccgcca atggttgacg gcgacagatt acggtttatg caaattttca ggtattcatc
     5701 aagataggac ttaatatttt tggttttctt tactacaccg aataactcca gttttttgga
     5761 attggggaaa tatttcgcat attcaaacgt tccgctggcg atctgatttt gtatctcccc
     5821 tagcaggcgc tcagcatatt ttataccacg tgtatttgcc tcaagccgag ataggggctc
     5881 cctgcaaaga acccctttat atgtgaaagt gataactagt gttgaagcag ttttatgctt
     5941 acgaatagtt actcctcttg gcagagataa taatccttgt tctttcttgc ccattttgaa
     6001 acctctatta agtcgaccca acgctcttta accccgtcga cttttaatac atgtacccct
     6061 tctttccata ttcctctttg tatccgtttg ttaacggcat caaccgtttc tcccgcgttg
     6121 cggcagtagg ttgatatagg tacgcaatcc agccccacag ttcacctcac acaacattca
     6181 gcccacgaca gtgacaccac acttcaaaca ttcgtttcac aatttcacga cagtagaaac
     6241 cgtcaacatc tcgcgtcagg tcatagcgat tgccgaatgt cttgcgaacc cataactcaa
     6301 aaaccgtatg catgtatcac ctccgttgca ttgcgcgcaa ttttttcagg tgcttttcct
     6361 gctcagtgtc cgccaggatt ttgcaatact gctggtggtc aatatgttcg aacaggcagt
     6421 tgaactcacc aatgcatacc cgcccggagt gtccgtccat acgtcgaaag aacacagagc
     6481 ggtccgtaca gcgagtaatc accacagggt atccggccct gtccgtgtat atttgcccgc
     6541 gttgaataag tgcgaacatg agattatccc cagcggcaaa tcgaaaaaat aatcagcgca
     6601 atcgatattg caacacctac aattgcaaat gcttcaggcc aactcattga ctcacctcct
     6661 gcggcggttc tggtagaggc atccagtggg ttacatcgca ttcagggatg ctgatatcat
     6721 caccaagcca cccttgacct tcagaccagc attgcacgta atacccgtat tctgtgttca
     6781 ctacgcacca ctgtgcgtcg ttcggcatta gctcactaca gcttatccaa ccatccggag
     6841 ttaccggaac ttgcggaatg gctgtctgct ctcgaacgtc attaggcgct ataggttctg
     6901 ctgccaactg actggcatat ttgttaatgg taacgataag ctcttgctca gcctcatcca
     6961 gataatcacc gatacctcgc ctgtcaccgt caaaatcatc gaaatcggca cgaatcttgg
     7021 caaccttctg gattgcggac aacacctcac taggaattac cggatagttg gttgacgttt
     7081 ccgcgatttc ccgaaaatta ttggttgacg aattcttatt ttcccgaaag tttctggcct
     7141 gaagcatggc ggcgcggcag gcattccagc cagtagcgta atatttcgca tcctgccagc
     7201 tcgtttgtac atttgagtac gctttttcct ctgtcatggc ctctggtatt gatgccggtg
     7261 caattcgtgg caggccgata tatagtggta catctcccgg ctccatcgaa ttgacgggcc
     7321 aaatatacgc atcactacca tggttaatga gcgcaatccc cgaagtatcg atgatgccaa
     7381 atggctccgc tgtcaatgca gtcaatgcaa tttcataagc ccgacgctca atattgtctc
     7441 gcacgtccag gctgcttatg cgctctttga tttctttaat cagtttctta tcggtgaaag
     7501 tggtcatctc actctccttt gatgcgaatg ccagcgacgc gtagtgcgtg ttctaagtca
     7561 atcaggcaaa gccagctgcc attttcttta ggtatcatga catgtcgctt atcagcattt
     7621 attgggtgcc catatcgaag gtcgtagcga gtcggtaatt gaacttccct cgcttccagt
     7681 tctgtaatgc gtttgttttt agcttctagt tcatccagca gcaccagcac aacctgcggt
     7741 gtgactttca tacgaaacgc aagcaatttt tgcggcgttg ccacgacttt tattgcttct
     7801 gccgcctcac gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttacggatct
     7861 gcgctgcgat gcgcgaaaaa aaagcctccc gcgtatggct gttaagagct ggcgcgaacg
     7921 ccgcgttaag aacggcagca tcacagccgt catcgatata gagcgcaatt tttttctcca
     7981 ggcgcgcttt ggcttcctgc aactgcatac cccggcacgc acgcgggata taatcagcaa
     8041 tttgagcgat agctttttcg ttctgtttaa acatgcttca cctcgatagg cttgatggtg
     8101 tctaacagca gtcggcggcg cgtattttct gcaaaatggc ggcgtccagt ttctttgtgg
     8161 taaaactcgt ttttgccaac gacccacatc cgctctgttt ggtgcaattt ttttacctgc
     8221 gggccatctt tggtgatcac ggtaccggta tgggttttta cgattgtcat gccacgacct
     8281 cttcgaattt caactccaat tggtcacccc agatttcaca tgattcggaa cacgaaccag
     8341 tatcaaaccg cttggccagc accatcgcct gatacaaact tctgtagtcg ctgtcggcat
     8401 acattctggc aatcccgtca agcgtcaggt gaccacggta cataacgtct ttacctgttc
     8461 tgcgatgacc atccctgacg tgtttgcctg taaccagttc attaaaaacc cgcatcagac
     8521 caggttcgtc tttacatgca agccccagct tttgcgttga ctttttgatg caaaaaacac
     8581 agttcccgag gtgctccggg atttgcaaat caaaaggttg tttttgccac caccggataa
     8641 catccgactt atcaaaatct gacagctcgg caagataccg gacgccaggt ttcggtttca
     8701 gcctacgggg ttcgtccgca cgaataccga gccatgtgat gtaattccct cgcccaaaat
     8761 ggtcatcgca atatttcgtg aaaggggtga gttttagcct gtcagtacag aatgcgccgc
     8821 cgatgtatgg cgtaccgtac tttttaacca tgtccataaa cggtttaagc accggcattc
     8881 gcgtctgaat atcctttggt tcccattccg tataaccatt tggctgccca agctctggat
     8941 ttatatcgac ctgcaacaca gttagcggta tgccccagaa cttcaaaacc tcccgaataa
     9001 agcggtatgt cagcggatgt tcgcaaccgg tatccataaa gatgtagcag acgttattgc
     9061 cagcctttct ttgttcttcc atcaggtgaa caagatatgc agatgttctc ccgccagaaa
     9121 aactaactac atgagttatg cacatttgcg taattccgat aactcgttga agcgttccat
     9181 aaacatcccg taggcatggc ccggtgccag tggaatcact ttgaacatct ctgttgccgg
     9241 gataccttcc aatacaggcc agaaagagcc atcatcaagt ccgagatcgc ggcgttcggt
     9301 tgccagcatg atgagatcgg catatttcac tggcgtgctc ataacaggag gtaacccgta
     9361 tttctcacgg attacggcgt ctattttttc ttccatccgt ttatagtcag gaagaagtcg
     9421 tttcagtggc gcggggatgt cctggcaata tgcttctgtt gcatcatgca ttaacgcttc
     9481 aaaagcaaat tcctgcggca ccagctggct gcaaagcacc gcatgctggg cgacactgta
     9541 gaagtgtgaa agatgtcctg caaagcgaca gatatttgaa agggaaactg cgatatcgtt
     9601 aatcacgatg tcgtctttat ttatcttgtc ataataaaaa tgcttcccgg aaaaagtttt
     9661 aataaatgac atttcgttct ccactttata tgcgctgcac cgcgctgaat tctgctaaaa
     9721 ggaagctctc accatccggt gattattgag ttaattacgt ttccataaat gcccccgcag
     9781 gggcatttgc agtaatgaaa tcaggcggtg aaagtaccaa taaaggtttc tactttgctg
     9841 tctttgaatt tctcaacaag cagatcacga aattcgttag ccatttcttc ctgca
//
LOCUS       DAZFKM010000169         9892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFKM010000169 DAZFKM010000000
VERSION     DAZFKM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(400..1593)
                     /locus_tag="ACHKAY_004498"
     CDS             complement(400..1593)
                     /locus_tag="ACHKAY_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO5703052.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1729..3453
                     /gene="iucA"
                     /locus_tag="ACHKAY_004499"
     CDS             1729..3453
                     /gene="iucA"
                     /locus_tag="ACHKAY_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO5703053.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGGCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEL"
     gene            3454..4401
                     /gene="iucB"
                     /locus_tag="ACHKAY_004500"
     CDS             3454..4401
                     /gene="iucB"
                     /locus_tag="ACHKAY_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO5703054.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4401..6143
                     /gene="iucC"
                     /locus_tag="ACHKAY_004501"
     CDS             4401..6143
                     /gene="iucC"
                     /locus_tag="ACHKAY_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO5703055.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6140..7477
                     /gene="iucD"
                     /locus_tag="ACHKAY_004502"
     CDS             6140..7477
                     /gene="iucD"
                     /locus_tag="ACHKAY_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO5703056.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7483..9678
                     /gene="iutA"
                     /locus_tag="ACHKAY_004503"
     CDS             7483..9678
                     /gene="iutA"
                     /locus_tag="ACHKAY_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO5703057.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2339 a         2581 c         2625 g         2347 t
ORIGIN      
        1 tccagctctt tcaggtgtca acgccactct gagagagttt tatgcatccc gtattgggca
       61 ctatgatgcc ggaaatctgc gaggctcgac acctctctga ttttcccctt tatttatcac
      121 ggtgccccag atctaatgtt ggggatgcgt tcatctcatt cccaatgagt aatcgcactg
      181 ttctcattgt ctgcaggcat ccggacagag ccatgtgatg ttgcactcac aaccagcatg
      241 acttcaatgc aacttaagtg acaccggccg gttttatacc gaacataccc tgtaccatgc
      301 ggtgcatttt tgccagtgtt actgcctgac atgctttcat cagacgcagg ttctctctca
      361 tatcctctga gttactccgg taaggtgatg atcatatctt tatttgatag cacggtaatg
      421 ccagataaat acagacacaa tggtaaataa tatagttgca aataatgccg ccataatcag
      481 cgcgattgcg acacctgttt tcgataccag cggcaccagc aaagcgcaca gtccatagcc
      541 aagtgtgtga ctggtagcga gccagcccgc gcctgcgcca tcagccagct tatcattcag
      601 tagaagttga taagcgggtg ttgccagagc tgctccaaat gacagcactg cacagcccgg
      661 gtaaaacaac catagctgtt cggagagcat gatagccaga ccaccactca tcaacactcc
      721 ggcactcagg agcagggcca ccggagtcag gcgctgcgga cggagtaccc cgaactgcgc
      781 gataagcgca gctactgcgg acagacccaa cagccaggcc acctgttggc taatggcggt
      841 ggtatccgtg gcgaactggc gagtaagggc aggcgaaagt ccaagctgca tcatgctcac
      901 cgccgcagcc agtaatattg cgcaaagcag ataaggcaga caatcccgct tcagactgac
      961 gctcttgcgc tccggtgtgg gctgtggtgg tgtgccgggc aaccgcagca gcatcagcag
     1021 cgccagcact ggggctgcca tcagcagccc cagtggcgcc agaggatgaa tggccagcat
     1081 tgctgccgcg cacagcggcc cgaatagcct gccgcaactc aggccggagc tgatggttgc
     1141 cagagcggcc atgcgattcc cttctcctgc tctctgcaat gcccagacct gacaggctgg
     1201 caccattgcc gacacggtca gcccgtaggc gatccgggcg atgattaata ttcccagtcc
     1261 caccgctgtt gttatggcgc tggtagccat cagcacgctt cccagcccta gcagggtaaa
     1321 gcttatgcca tatcctccca gagcccacaa caccacaggt ttactgccag tacgggaaat
     1381 ttgctttccc caccacgggg acgatggcag aaacagcatt gagccaagca tcagcagggc
     1441 agcccataca gacagactca gatttgtctg tatgaccaga acagggagca caaccagcag
     1501 accgttctgc ccgataccga gaagcccggc actgaacgca agtggccagc aggacagtgg
     1561 tttttggggc gtatcttcga tcccaggtga cattttaatg tttcaactcc atgtattaat
     1621 tgtgtttatt tgtaaaatta atttatctga caataacatt tcttattgat aatgagaatc
     1681 attattgaca taattgttat tattttactg tgtgggagct gtttgactat gaccctgccc
     1741 tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc tgaatgcact gattcgtgaa
     1801 actacagact ggaaactgac agaatacccg ccagacgaat tgatcatccc gctggatgag
     1861 cagaaatcgc tccatttcag agtggcttat ttctccccaa cccagcatca ccgctttgca
     1921 tttcctgcac gtctggtcac ggcatcaggc agttatcctg tcgactttac cactctctcc
     1981 cggcttatta ttgataagct acggcatcaa ctgtttctgc ccgttcccct ctgcgaaact
     2041 ttccaccagc gcgtgctgga aagccacgcc catacgcaac aggcaattga tgcccgtcat
     2101 gactggaccg ccctgcgtga aaaagcgttg aattttggcg aagctgagca ggcgctgctg
     2161 acaggacacg ctttccaccc tgcgcctaag tctcatgaac cgtttaaccg gcgggaggct
     2221 gaacgctacc tgcctgatat ggcaccccac ttcccactgc ggtggttttc ggtggataaa
     2281 acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac agcggctgac gcgatttgcc
     2341 gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca accaatggct gttcccgttg
     2401 cacccgtggc agggggaata tcttttgcag cagggggggt gccaggcact tgttgctaag
     2461 gggctgatta aagacttagg tgaggccggc acgtcgtggc tgccgaccac ctcttcccgt
     2521 tccctctact gtgccaccag ccgcgatatg atcaagttct ccctgagcgt acggctgacc
     2581 aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg ggatgcgcct ggcacgcctg
     2641 gctcaaaccg acggctggca gatgctacag gtccgcttcc cgactttccg ggtaatgcag
     2701 gaggatggct gggccgggct gctcgatctt aacggcaaca tcatgcagga aagtctgttt
     2761 gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc agaccaacgt actggtctcc
     2821 ctgactcagg ccgcacctga tggcggtgat tcgctgctgg tttcggcggt aaaacgcctg
     2881 agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg catgggtcga tgcatactgt
     2941 cagcaagtgc taaagccgct gtttacggct gaagcggatt acggcctggt gctgctggcg
     3001 catcagcaaa atattcttgt ccagatgctt ggggatctgc cggtcggatt tatttaccgt
     3061 gactgtcagg gcagcgcttt tatgcctcac gcgacagact ggctcgattc tattggcgag
     3121 gcgcaggcgg aaaatatttt cacccatgag cagttgctgc gctatttccc ttattacctg
     3181 ctggttaact ccacttttgc tgtgaccgct gcgctggggg ctgccgggct ggacagcgaa
     3241 tcgaatctga tggctcgtgt acgagcatcg ctggctgaag tgcgtgatca ggtgactcat
     3301 aaaacctgtc tcaactacgt gctggaaagt ccgtactgga acgtaaaagg taactttttc
     3361 tgttatctga acgatcataa cgagaacacc attgttgacc cttcggtgat ctatttcgat
     3421 ttcgctaacc cgctgcaggc tcaggagctc tgaatgtctg aggcaaacat tattcacagc
     3481 agatatggac tgcgctgtga aaaactcgac aagcccctga atcttggctg gggactggac
     3541 aatagcgcgg tgttgcactg tcccggggag ctgccgacag ggtggctgtg cgatgcgctg
     3601 gatcagatat ttatcgccgc accacaactg tcagcagtgg ctctcccctg ggccgaatgg
     3661 tgtgaggagc cacaggcgct gacgcttttt gggcaggtaa aaagcgacat tatccatcgt
     3721 accgccttct ggcagttacc gttatggttg agttcaccgg caaaccgggc ctccggtgaa
     3781 atggtttttg atgcagagcg tgagatttat ttcccgcagc gcccaccccg tccgcagggt
     3841 gaggtttacc gccgttacga tccacgtatt cgcaggatgc tgagtttccg cattgccgat
     3901 cccgtttctg atgcagaacg tttcacccgc tggatgaatg atccgcgcgt tgagtatttc
     3961 tgggagcaaa gtggctcact ggaggtacag accgcctatc tggagcgcca gctcaccggt
     4021 aaacatgcat tcccgctgat tgggtgcttc gacgatcggc cattcagcta ttttgaaatc
     4081 tactgggcgg cggaagaccg cattggtcgt cactattcgt ggcagccctt tgaccgtggc
     4141 ctgcatctgc tggttggtga acagcaatgg cgcggggccc actatgtgca aagctggctg
     4201 cgcgggctga cacattacct gctgctggat gagccccgta cgcagcgcac agtactggag
     4261 ccacgcaccg ataaccagcg cctgttccgc catcttgagc ctgcgggata ccggacaatt
     4321 aaagagttcg acttcccaca taagcgctcg cgcatggtga tggcggatcg tcatcacttc
     4381 ttcacggagg tcggtctgta atgaatcaca aggactggga ttttgttaac cgtcggctgg
     4441 tggcaaaaat gttgtctgag atggagtatg agcaggtttt ccacgcggaa tctcagggcg
     4501 atgaccacta ctgcattaac ctgccgggag cacaatggcg ctttatcgct gaacgtggta
     4561 tctggggctg gctgtggatt gatgcccaaa cgttgcgctg cacggacgaa ccagtactgg
     4621 cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat gagtgatgcc actgttgcag
     4681 agcatatgca ggatttgtat gccacgctac tgggcgacct gcaactgctg aaggcccgtc
     4741 gcggactgag tgccagtgac ctgattaatc ttgatgccga tcgtctgcaa tgcctgctga
     4801 gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg ctggggcaaa gaggcgctgg
     4861 aacgatatgc gccagagtat gccaacacct tcagactgca ctggctggcg gtaaaacgtg
     4921 aacatatgat ctggcgctgc gacaacgatc tggatattca gcagttgttg actgccgcaa
     4981 tggatccgca ggagtttaca cgcttcagtc aggtctggca ggaaaacggg ctggatcaca
     5041 actggctgcc gctgccggta catccgtggc agtggcagca aaaaatcgcc accgacttca
     5101 tcgctgattt tgccgaaggc aggatggtgt ctctcggcga gtttggcgac cagtggctcg
     5161 cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg gggagggctg gatatcaagc
     5221 tgccgctgac catctacaac acctcatgct accgggggat ccctggcaga tatatcgctg
     5281 ccggaccact ggcttcacgc tggctgcaac aggtttttgc gaccgacgcc accctggtgc
     5341 aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta tgtgtcccat gaaggctatg
     5401 ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct tggtgttatc tggcgggaga
     5461 atccgtgccg ctggctgaaa ccggatgaaa gtccggttct gatggcaaca ctgatggagt
     5521 gcgacgaaaa caatcagccg ctggcaggcg catatatcga ccgctccgga ctggacgctg
     5581 aaacctggct tacgcaattg ttccgggtgg ttgtggttcc gctgtatcac ctgctttgcc
     5641 gctacggtgt cgcgcttatt gcacacggac aaaatataac gctcgccatg aaagaggggg
     5701 taccacagcg tgttctgctg aaagacttcc agggcgatat gcggctggtg aaagaagcgt
     5761 ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt cacatcccgt ctgagtgcgg
     5821 actacttaat ccatgatttg cagacgggtc acttcgtgac agtactgcgt tttatttcgc
     5881 cactgatggt tcgtcttggc gtacctgaaa ggcgatttta tcaactgctg gcagcagtgt
     5941 tgagtgatta catgaacaaa catccacaaa tgtcagagcg ttttgcgctt ttctcactct
     6001 tcaggccaca aatcattcgc gtggtgctga acccggtaaa actgacctgg ccggaccagg
     6061 atggcggcag tcgcatgctg ccgaattacc ttgagaatct gcaaaatccg ctgtggctgg
     6121 taactcagga atatgaatca tgaaaaaaag tgtcgatttt attggtgtcg ggacagggcc
     6181 atttaatctc agtattgctg cattgtcaca tcagatcgaa gaactgaact gtctcttctt
     6241 tgacgaacat cctcattttt cctggcatcc gggtatgctg gtaccggatt gtcatatgca
     6301 gactgtcttt ctgaaagatc tggtcagtgc agttgcacct acaaatccct acagctttgt
     6361 taactatctg gtgaagcaca aaaagttcta tcgcttcctt acaagcagac tacgtacagt
     6421 atcccgtgaa gagttttctg actatctccg ctgggctgct gaagatatga ataacctgta
     6481 tttcagtcat accgttgaaa atattgattt cgacaaaaaa agtcgattgt ttctggttca
     6541 gaccagtcgg ggagaatatt ttgcccgcaa tatctgcctt ggtacaggaa aacaacctta
     6601 tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc catgccagtg aaatgaatct
     6661 tcgtcggccg gacctgagtg gaaaacggat aactgtggtt ggtggaggac agagtggtgc
     6721 agacctgttc cttaatgcat tacgcgggga atggggagaa gcggcggaaa taaactgggt
     6781 ctcacggcgt aataatttta acgcactgga tgaggctgct tttgctgatg agtattttac
     6841 acctgaatat atttcaggct tctccggact gaaggaagat attcgccatc agttactgga
     6901 tgagcagaaa atgacatcgg atggcatcac tgccgattct ttactgacca tttatcgtga
     6961 gttgtaccac cgttttgaag ttctgagaaa accaagaaat atccgtctgc tacccagccg
     7021 ctcggtaaca actctggaaa gtagtggtcc gggctggaag ttattgatgg aacatcatct
     7081 ggatcggggc agagagagcc tggaaagtga tgtggtgatt ttcgccacag gttaccgttc
     7141 tgcattgcca caaatacttc cctcactgat gcccttgatc accatgcacg ataagaacac
     7201 ctttaaagtg cgtgatgact tcactctgga atggagtggc ccgaaagaaa ataacatctt
     7261 tgcggtcaac gccagcatgc aaactcatgg catcgccgaa ccccagctca gcctgatggc
     7321 ctggagatct gcacgtattc ttaatcgcgt actggggcgt gatttattcg atctcagtat
     7381 gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa aaaccgcagc cggaggctgc
     7441 tgctttaact cactatacaa caaatattca ggaataacga tgatgcgcaa aaagtatatg
     7501 ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac cagctgtcgc ccagcaaaac
     7561 gatgataatg agatcatagt gtctgccagc cgcagcaatc gaactgtagc ggagatggcg
     7621 caaaccacct gggttatcga aaatgccgaa ctggagcagc agattcaggg cggtaaagag
     7681 ctgaaagacg cactggctca gttaatcccc ggccttgatg tcagcagcca gagccgaacc
     7741 aactacggta tgaacatgcg tggccgcccg ctggttgtcc tgattgacgg tgtgcgcctc
     7801 aactcttcac gttccgacag ccgacaactg gactctgtcg atccttttaa tatcgaccat
     7861 attgaagtga tctccggcgc gacggccctg tacggtggcg ggagtaccgg agggttgatc
     7921 aacatcgtga ccaaaaaagg ccagccggaa accatgatgg agtttgaggc tggcacaaaa
     7981 agtggcttta acagcagtaa agatcacgat gagcgcattg ccggtgctgt ctccggcgga
     8041 aatgaccata tctccggacg tctttccgtg gcatatcaga aatttggcgg ctggtttgac
     8101 ggtaacggcg atgccaccct gcttgataac acccagaccg gcctgcagca ctccaatcgg
     8161 ctggacatca tgggaaccgg tacgctgaac atcgatgaat cccggcagct tcaactgata
     8221 acgcagtact ataaaagtca gggggacgac aattacgggc ttaatctcgg gaaaggcttt
     8281 tccgccatca gcgggagcag cacaccatac gtcagtaagg ggctgaattc tgaccgcatt
     8341 cccggcactg agcggcattt gatcagcctg cagtactctg acagtgattt cctgagacag
     8401 gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc ggttctaccc gttcccgacg
     8461 gtaaatgcga ataaacaggc gacggctttc tcctcgtcac agcaggatac cgaccagtac
     8521 ggcatgaaac tgactctgaa cagccaactt atggacggct ggcaaatcac ctgggggctg
     8581 gatgctgagc atgagcgctt tacctccaac cagatgttct tcgatctggc tcaggcaagt
     8641 gcttccggag ggctgaacaa ccataagatt tacaccaccg ggcgctatcc gtcatatgac
     8701 atcaccaatc tggcggcctt cctgcaatcc agctatgaca ttaatgatat ttttaccgtc
     8761 agcggtggcg tacgctatca gtatactgag aacagggtag atgattttat cgactacacg
     8821 cagcaacaga agattgctgc cgggaaggcg atatctgccg acgccattcc tggtggttcg
     8881 gtagattacg ataactttct gttcaatgct ggtctgctga tgcacatcac cgaacgtcag
     8941 caggcatggt tcaatttttc ccagggggtg gcattgccgg atccggggaa atattatggt
     9001 cgcggcatct atggtgcagc agtgaacggc catcttcccc tgacaaagag cgtgaacgtc
     9061 agcgacagta agctggaagg cgtgaaagtc gattcttatg aactgggctg gcgctttacc
     9121 ggtgacaacc tgcggactca aatcgcagca tattactcgc tttccaataa gagcgtggaa
     9181 aggaataaag atctgaccat cagtgtgaag gacgacaggc gccgtattta cggcgtggaa
     9241 ggtgcggtgg actacctgat cccggatact gactggagta ccggtgtgaa cttcaatgtg
     9301 ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat atgacgtgaa ggaatcaagt
     9361 ccatcgaaag cgacagctta cattagctgg gcgccggaac cgtggagtct gcgtgtgcag
     9421 agcaccactt ctttcgacgt aagcgatgca gagggtaacg atattaatgg ttacactacc
     9481 gtcgatttta tcagtagttg gctgcttccg gtgggaacac tcagcttcag cgttgagaac
     9541 ctcttcgacc gtgactatac cactgtctgg ggacagcgtg cacctctgta ctacagcccg
     9601 ggttacggcc ctgcttcact gtacgactac aaaggccggg gccgaacctt tggtctgaac
     9661 tactcagtgc tgttctgacg gggattcttt tgcaacaagg gtatgctgta aacaacatgg
     9721 tccttggtgg ccatgttgtt tacagccagt ataatataca gccttaacaa gatattccgt
     9781 ctccggaatg gactgttcac cactgaataa caatatgtct gttttatgga tctttgactg
     9841 aaatttatcg catatcctca tttcattgtc cgtctgaaaa ctaaccttac aa
//
LOCUS       DAZFKM010000170         9874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFKM010000170 DAZFKM010000000
VERSION     DAZFKM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..609
                     /gene="lpfA-O113"
                     /locus_tag="ACHKAY_004504"
     CDS             37..609
                     /gene="lpfA-O113"
                     /locus_tag="ACHKAY_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO5703058.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            657..1388
                     /gene="lpfB-O113"
                     /locus_tag="ACHKAY_004505"
     CDS             657..1388
                     /gene="lpfB-O113"
                     /locus_tag="ACHKAY_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO5703059.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1413..3935
                     /gene="lpfC-O113"
                     /locus_tag="ACHKAY_004506"
     CDS             1413..3935
                     /gene="lpfC-O113"
                     /locus_tag="ACHKAY_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO5703060.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3946..5019
                     /gene="lpfD-O113"
                     /locus_tag="ACHKAY_004507"
     CDS             3946..5019
                     /gene="lpfD-O113"
                     /locus_tag="ACHKAY_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO5703061.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            5267..6307
                     /gene="pstS"
                     /locus_tag="ACHKAY_004508"
     CDS             5267..6307
                     /gene="pstS"
                     /locus_tag="ACHKAY_004508"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO5703062.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6394..7353
                     /gene="pstC"
                     /locus_tag="ACHKAY_004509"
     CDS             6394..7353
                     /gene="pstC"
                     /locus_tag="ACHKAY_004509"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO5703063.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     IAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7353..8243
                     /gene="pstA"
                     /locus_tag="ACHKAY_004510"
     CDS             7353..8243
                     /gene="pstA"
                     /locus_tag="ACHKAY_004510"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO5703064.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8334..9107
                     /gene="pstB"
                     /locus_tag="ACHKAY_004511"
     CDS             8334..9107
                     /gene="pstB"
                     /locus_tag="ACHKAY_004511"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO5703065.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9122..9847
                     /gene="phoU"
                     /locus_tag="ACHKAY_004512"
     CDS             9122..9847
                     /gene="phoU"
                     /locus_tag="ACHKAY_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO5703066.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2540 a         2265 c         2518 g         2551 t
ORIGIN      
        1 tcatggtaaa ggatatatta tatcaatgga gtttttatga agcgtaatat tataggcggt
       61 gcattcactc tggcatctct aatgctggcc gggcatgcac tggcagaaga tggtgttgtt
      121 cacttcgtcg gtgaaattgt cgacactact tgtgaagtta cctccgatac agccgatcaa
      181 attgtcccac tgggtaaagt cagtaaaaat gcattttcag gtgtaggtag tctggcgtcg
      241 ccacagaagt tcagtattaa gcttgaaaat tgcccggcaa cgtacactca agcagccgtt
      301 cgttttgatg gtacagaagc gcctggcggc gacggcgacc tgaaagtggg tacgccgctt
      361 acagcaggca accctggtga ttttaccggt acaggacaag cgattgcggc aaccggcgtt
      421 ggtattcgta tttttaacca gtccgataat tcgcaggtta aactttataa cgactctgct
      481 tataccgcta tcgatgctga aggcaaggct gaaatgaagt ttattgcacg ctatgtggca
      541 accaatgcga ccgtaacggc tggtacggcg aacgctgatt cacaatttac tgtcgaatat
      601 aagaaataat ttatcaaggg cctttttatt cagttggctt tggccaggga gtcgttatgt
      661 ccagaaaaag ttgtttaatc aggctcttta aaacaacagt ttcatgtaag ttatttaccg
      721 cgattatact cagcgttttt ttaggccaac cagcattaac ttatgcaggc gttgtcattg
      781 gtggtacgcg ggtggtttac ttaagtaata atgctgataa atctatttct gtatttagca
      841 aagaagaaaa aataccttat ctcatccagg cttgggtaga tccatttaat aaagaggata
      901 aaagcaaagc gccgtttacc gttattccgc cagtatcacg attagaacct tcgcaagaaa
      961 aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc gagtcagtct
     1021 tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg aactcgctgg
     1081 aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc cgccttattc
     1141 ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg atagcagaga
     1201 atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat gatgtgcccc
     1261 tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat tctgcgggag
     1321 gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg ataaaaatga
     1381 cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata gtggttggcc
     1441 tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc agtgtattca
     1501 atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag tttaatcggg
     1561 caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt aaaatgtttg
     1621 aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac gcttgcttta
     1681 ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc aaatcgttca
     1741 atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc tcatcctggc
     1801 agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac atggacgcga
     1861 tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg ctcaccatca
     1921 actacgattt ttctggttca catacactac gttcagatta tggttcacaa gagacagata
     1981 ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta cgtaattata
     2041 gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc tggatacagc
     2101 gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg gcgagcgata
     2161 ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat atgctacccg
     2221 ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac gccaccgtca
     2281 tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt gcttttgaga
     2341 tcaccgatct caacaccgcc agtacaggtg gcgatttgga cgtaaccatc aaagaagaag
     2401 acggtagcga acaacgattc acccaacctt atgcttcatt ggcgattctt aaacgtgaag
     2461 gtcagacaga tgttgatgtc agcgtgggtg aattgcgcga tgaagacgga tttacaccgg
     2521 acgtccttca ggcgcaaata cttcatggtt tttcccacgg gatcacttta tatggaggta
     2581 tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt cggtaaagat cttggcgctt
     2641 tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa ttttagccat gatgatacag
     2701 aaacgggtca gtcatatcgc tttctctatt caaaacgatt tgacgacaca gacactagct
     2761 tgcgcctggt tggctatcgt tactccaccg agggctacta taccctcaat gagtgggcat
     2821 cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa ccgacgtagt cgcgtggagg
     2881 gaacgctaac gcagtcgttg gggagagatt atggcaattt atacctgaca ttaagccggc
     2941 aacaatactg gcataccgat gatgtcgaac gattaatgca atttggctac agcagtagct
     3001 ggaagcgtct ctcgtggaac gtctcctgga gttattccaa tactgccaga caggggacgg
     3061 ggaacaacca tgccagtgat aacaccagtg agcagatcta catgctctct ttatctgttc
     3121 ctttatcggg ctggtggggt aatagttacg ccacctattc tgtttcgcaa aacgataatt
     3181 ccggtagctc acatcaactc ggactcagcg gtacggcgct ggaacgaaat aacctttcat
     3241 ggaatttaat gcagtcctat aacagtcatg atgatgaggt tggcggtaat atgtccctga
     3301 cctatgatgg ctcttatggc acggtgaacg gcagctataa ctacagccaa aattcccaga
     3361 ggctgaatta tggtatcaga gggggaattc tggcacacag cgaaggggta acgttaagtc
     3421 aggagttagg tgaaactatt gctcttgtta aagcacctgg ggccgccggg ttagaaatag
     3481 ataatatgcg cggtgctgcg acggactggc gcggctatac ggtcaagaca cagctaaacc
     3541 cttatgatga aaatcgggta gcaatcagcg ataactattt ctcgaagtcg aatatagaac
     3601 ttgataatac cgtcgttacg atggtttcca cgcgtggtgc agtggttaaa gcggagtttg
     3661 tgactcatgt gggttatcgc gttctcttca gagtgttaaa tgcaaatggt aaaccggtac
     3721 cttttggagc cattgctgcg atacaagatg caagtttggc agattcagga attgtcggtg
     3781 accgtggcga actttatctt tctggtctac cagaaaaagg acaggttacg ttatcctggg
     3841 gagaaaacgc ctcaacaaaa tgcatcttca attattcact ttcgacacca gaaagtgaga
     3901 gcggattaat tgaacagggt gtgacatgtc attaaaagga tttttatgaa caagtatatc
     3961 aaacagtggt gctttgctgt gtttatgctc tcgttaagta gtgtagcact tgcagctcct
     4021 aaaggtatct gtacccctga taatggcgta tttcacagta cgcttgattt ctcgggatat
     4081 cttattaccg cgaacgaaaa taaggttgga acgacattta acaccactgt aactaatgga
     4141 tcctcatatc ctggacgctg tcattgtgat actggtaacg taggagaatt cccctatatc
     4201 tattatacgt caaaaataaa ccaggcatta acttatgctg gggttcactc taatattaat
     4261 tattatgatc ttaatccgaa tcttgatgtt gggattgcta tagatattct tggggttggg
     4321 tatgtgaatg caccttttga atatcatgct aacaacccgt ctggtaatac aaagtacaat
     4381 tgcaaccgca ttgaaccttt aagtatatcc agtggggcta aagcgatagt atatttttat
     4441 atcaagaaga catttgcagg aaaattgatt attcctgaaa caaagatagt gacattgtat
     4501 ggaacaatta gccgtgacac cccggtggat tactcacagc cgatggctga tgtttatatt
     4561 cggggcgata ttacagctcc gcaaagttgc gaaataaata atttacagcc agtttatttt
     4621 gattttaaag aaataccagc tgcagatttt tcatctgttg ttggaagtgc ggtaacaaca
     4681 cataaaatta ctaaaacggt cactattgag tgtgaaaacc tggggatact gaataccgat
     4741 gatatcagta cgtcttttta tgctaccgaa cccaatacgg acaattcaat ggtcgtaact
     4801 tcaaactcta acgtggggat aaaaatttac gacaaaaata ataaggaaat caaagtgaac
     4861 ggtggtgagt tgccaacaga catgggtaaa tcaacagtgt atggtgaaaa atcaggtagt
     4921 gtaacttttt cagctgctcc tgcgagcctt actggagctc gcccatcccc ggggcaattt
     4981 actgcaacag cgacgataac tgttgagatt gtgcgctaat aggtctgaca acgattgttc
     5041 atcacatcgt tgtcatctca cccatttctt tatcactttt aaatgcatca tgacaaaaag
     5101 atgacatgtg ggattttcat cttcacattt aaaatcagta agttatttgg taattccttt
     5161 ctgtcataaa aatgtcatat tccttacata taactgtcac ctgtttgtcc tattttgctt
     5221 ctcgtagcca acaaacaatg ctttatgaat cctcccagga gacattatga aagttatgcg
     5281 taccaccgtc gcaactgttg tcgccgcgac cttatcgatg agcgctttct ctgtgtttgc
     5341 agaagcaagc ctgacaggtg caggtgcaac cttccctgcg ccggtgtatg ccaaatgggc
     5401 tgacacttac cagaaagaaa ccggtaataa agttaactac cagggtatcg gttcttccgg
     5461 tggcgtaaaa cagattatcg ctaataccgt tgattttggt gcctctgacg cgccgctgtc
     5521 tgacgaaaaa ctggctcagg aaggtctgtt ccagttcccg accgtgattg gcggcgtggt
     5581 gctggcggtt aacattccag ggctgaagtc tggcgaactg gtgctggatg gtaaaaccct
     5641 cggcgacatc tacctgggca aaatcaagaa gtgggatgat gaagccatcg ccaaactgaa
     5701 tccgggtctg aaactgcctt cacaaaacat tgctgtagta cgccgcgcag atggctccgg
     5761 gacttccttc gtcttcacca gctacctggc gaaagtgaac gaagagtgga aaaacaacgt
     5821 tggtactggc tctaccgtaa aatggccgat cggtctgggc ggtaaaggta acgacggtat
     5881 cgccgcgttc gttcagcgtc tgccgggtgc aattggttat gttgaatatg cttacgcgaa
     5941 gcagaacaac ctggcgtaca ccaaactgat ctccgctgat ggtaaaccgg ttagtccgac
     6001 cgaagaaaac ttcgctaatg cagcaaaagg tgcagactgg agcaaaacct tcgctcagga
     6061 tctgaccaac cagaaaggcg aagatgcatg gcctattacc tctaccacgt tcattctgat
     6121 ccacaaagat cagaagaaac cagaacaagg cacagaagtg ctgaaattct tcgactgggc
     6181 gtacaaaacc ggggctaaac aggcgaacga cctggattac gccagcctgc cggatagtgt
     6241 agttgaacag gttcgcgctg cgtggaagac caatattaaa gacagtagcg gtaagccgct
     6301 gtactaataa aactccaggc cgggtacggt gttttacgcc gcatccggca ttacaaaatg
     6361 actttgtaaa cgcgtttaac tgaagagtaa cttatggctg caaccaagcc tgcttttaac
     6421 ccaccgggta aaaagggcga cataattttc agcgtgctgg taaaactggc ggcgctgatt
     6481 gtgctattga tgttgggtgg cattattgtc tctctgatca tctcctcctg gccgagcatt
     6541 cagaaatttg gtctggcttt cctatggacc aaagagtggg atgcaccgaa cgatatctac
     6601 ggggcgctgg tgccgatcta cggtacgttg gtgacttcgt ttatcgcgct gctgatcgcc
     6661 gtcccggtga gtttcggtat cgccctgttc ctgactgaga ttgcgcctgg ctggctgaaa
     6721 cgcccgctgg gtatcgccat tgagctgctg gcagccattc caagtatcgt ttacggcatg
     6781 tggggcctgt ttatctttgc gccgctgttc gccgtttact ttcaggagcc ggtcggcaat
     6841 atcatgtcga atatcccgat tgttggcgcg ctgttctctg gccccgcatt tggtatcggt
     6901 atcctcgcgg caggcgtgat cctcgccatc atgattattc cgtacattgc ggcggtaatg
     6961 cgtgatgtgt tcgaacaaac cccggtgatg atgaaagagt cggcctacgg tattggctgc
     7021 accacctggg aagttatctg gcgtatcgtt cttccgttca ccaaaaatgg tgttatcggc
     7081 ggcatcatgc tggggctggg ccgcgcgctc ggtgaaacca tggcggtgac ctttatcatc
     7141 ggtaacacct accagctcga cagcgcctcg ctgtatatgc cgggcaacag tatcacctct
     7201 gcgctggcga acgaatttgc ggaagcggaa tccggtctgc acgttgccgc actgatggaa
     7261 ctgggcctga tcctgtttgt gattaccttc atcgtcctcg ccgcatcgaa gtttatgatt
     7321 atgcgcctgg ctaagaatga gggggcacgc taatggctat ggttgaaatg caaaccactg
     7381 cggcgctggc tgaatctcgc cgcaaaatgc aggcgcgtcg ccgcctcaaa aaccgtattg
     7441 cgctgacgct ctcgatggcg acgatggcct tcggcctgtt ctggctgatc tggattttaa
     7501 tgtccaccat cactcgcggt atcgacggta tgtcgctggc gctgttcact gaaatgacgc
     7561 cgccgcccaa tacggaaggt ggtggtctgg cgaacgccct ggcgggtagc gggctgttaa
     7621 ttttgtgggc cacggtattc ggtacgccgc tgggcattat ggcggggatt tatctggcgg
     7681 aatatggtcg taaatcctgg ctggcagaag tgattcgctt cattaacgac attctgctct
     7741 ccgcgccgtc gattgtggtt ggtctgtttg tctacaccat cgtggtggca cagatggagc
     7801 acttctccgg ctgggcgggc gtaattgcgc tggcgctgtt gcaggtgccg attgttatcc
     7861 gcaccaccga gaacatgctg aaactggtgc cggacagcct gcgtgaagcg gcttatgcgc
     7921 tgggtacacc gaagtggaag atgatctccg cgattacgct gaaagcgtcg gtgtccggga
     7981 tcatgaccgg tatcttgctg gcgattgccc gtattgctgg tgaaaccgcg ccgctgctgt
     8041 ttaccgcgct ctccaaccag ttctggagca cggacatgat gcagccgatc gccaacctgc
     8101 cggtgacgat ctttaagttt gcgatgagtc cgtttgcgga atggcagcaa ctggcctggg
     8161 ccggggtatt gatcattacc ctgtgcgtac tgctgctgaa cattctggcg cgcgttgttt
     8221 ttgcgaagaa taaacacggt taacactttg ccggatgcgg cgtgaacgcc tgatccggcc
     8281 tacggtaagc ctgattagcg aagtgcatca ggcacgatga ggaaaagatt gcaatgagta
     8341 tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac tacggcaaat
     8401 tccatgccct gaaaaacatc aacctggata tcgctaaaaa ccaggtaacg gcgtttatcg
     8461 ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg tttgaactgt
     8521 acccggagca gcgtgcggaa ggtgaaattc tgcttgatgg cgacaacatc ctgaccaact
     8581 ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaaa ccgacgccgt
     8641 ttccgatgtc catctacgac aacatcgctt ttggcgttcg tctgtttgag aagctctccc
     8701 gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa agccgcattg tggaacgaaa
     8761 ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa cagcgtctgt
     8821 gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa ccgtgttcgg
     8881 cgctcgaccc tatctctacc gggcgtattg aagagctgat caccgaactg aagcaggatt
     8941 acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc gaccacacgg
     9001 cgtttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg ttcaccaagc
     9061 cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc aggagtgcgt
     9121 tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg aactggaaag
     9181 tatccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt ctgatgcaat
     9241 caccgcgatg cataaccagg acagcgatct ggcgaagcgc gtgatcgaag gcgacaagaa
     9301 cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattatcg ccaaacgtca
     9361 gccgacggcg agcgacctgc gactggttat ggtgatcagt aaaaccattg ccgagctgga
     9421 gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg ctggagaaat tctcccagca
     9481 gcatcagccg ttgctggtaa gtctggagtc gctgggccgt cataccatcc agatgctgca
     9541 cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta tttatcgtga
     9601 agataaaaaa gtcgatcagg aatacgaagg tattgttcgt caactgatga cctacatgat
     9661 ggaagattcg cgtaccattc cgagcgtact tactgcgctg ttctgcgcgc gttctatcga
     9721 acgtattggc gaccgctgcc agaatatttg tgagtttatc ttctactacg tgaaggggca
     9781 ggatttccgt catgtaggcg gtgatgagct ggataaactg ctggcgggaa aagatagcga
     9841 caaataattc accagacaaa tcccaataac ttaa
//
LOCUS       DAZFKM010000171         9738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFKM010000171 DAZFKM010000000
VERSION     DAZFKM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHKAY_004513"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="ACHKAY_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO5703067.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="ACHKAY_004514"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="ACHKAY_004514"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO5703068.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="ACHKAY_004515"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="ACHKAY_004515"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO5703069.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(4357..4491)
                     /locus_tag="ACHKAY_004516"
     CDS             complement(4357..4491)
                     /locus_tag="ACHKAY_004516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703070.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="ACHKAY_004517"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="ACHKAY_004517"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO5703071.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="ACHKAY_004518"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="ACHKAY_004518"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO5703072.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="ACHKAY_004519"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="ACHKAY_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO5703073.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /locus_tag="ACHKAY_004520"
     CDS             complement(8992..9609)
                     /locus_tag="ACHKAY_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO5703074.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
BASE COUNT         2140 a         2479 c         2758 g         2361 t
ORIGIN      
        1 attccactcc gcgccgctca tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca caatttatgt gggtactgac cgtaaacccg
      421 catagtttac cgtacaggcg ttaccgtgac atcgtgtaat gcacctgtcg gcgtgataat
      481 gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaacacaat gtttactctg gtgcctgaca tttcaccgac
      601 aaagcccagg gaacttcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccg atccatcttt ccagcacata
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgcg gcaacccaac
      781 gcgcgatgtg gttcagcgtg cgctggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aatgaccgtc tttttgaaac ctggcgatct
      901 gctggttgcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgcggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 actggcagaa aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttgggtgccg atctggtgtt
     1201 gcattcatgt acgaaatatc tgaacggtca ctctgacgtg gtggctggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgtatgga
     1381 gctggcgcag cgcaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgtcagca
     1501 aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgggc gggctgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatttcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacttg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtgggccacg gggaagtatg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gtcagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct agtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtta tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgccac cgtcggtgcg
     3601 ggcttgccga tcaaccacac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagcggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
     3781 gacgacctct ccggcaaaga cgtaatgcgc aagctggtaa ttctggcgcg tgaagcgggt
     3841 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaat
     4021 ggcaaagcgc gtgtgggcgt ggaagcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccatgcgata acgtctttgc catcgaaagc cgctggtacc gcgataatcc gctggtgatc
     4141 cgtgggcctg gcgctgggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtc
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatga agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aatggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ctgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagccg gaaatgtatg cttctgacct ggtgacgctg
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaagt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgctcaac ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt ctttgatgtc gaaagctacc tgcgttttcg tgaccgctgc
     5161 gtatcggcgg gcattgatgt ggaaattatt ccggggattt tgccggtatc taactttaaa
     5221 caggcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcacaaatg
     5281 tttgacgggc tggatgacga tgctgaaacg cgcaagctgg tgggcgcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acactgtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgtaaa aataaggttg tcagctttgt tttcgccctc cttccttgaa
     5581 gaataggaat ctaaaaccct tttttataaa gcagttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatattcata acacgacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggcctgccga
     6061 ctggggcagc tacgccggtc tgtttattcg tatggcctgg cacggcgcgg ggacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcgggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgacc caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgtgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct tattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaatgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggttgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 cctgttcaag tatgagtggg tacagacccg cagcccggct ggcgcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gtttgatccg tcgaagaaac gtaaaccgac
     6901 gatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg accgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tttggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggc cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct
     7621 ggtgggcggg atgcgtgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg accgacgaat cgaaagagct gtttgaaggc cgtgaccgcg aaaccggcga
     7801 agtgaaatac actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtggctgaa gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgcct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtgt ccacaggaaa
     8041 actacacact ggatctctca tgtctgtcgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcga tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgtct
     8581 gtatgcccgt catccgcgcg tggatttatt gtcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccaa tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt agagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtaggagtt
     9721 tcagattact ggcacaga
//
LOCUS       DAZFKM010000172         9723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFKM010000172 DAZFKM010000000
VERSION     DAZFKM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..268
                     /locus_tag="ACHKAY_004521"
     CDS             <1..268
                     /locus_tag="ACHKAY_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFO5703075.1"
                     /translation="EAADFSDALMWLKDGKKVARRGWNGENQFCWLVPEGQYPARMEA
                     IKGYFPGDLVPYGAYFALKNAQGVVVPWVPSVGDLLACDWFVVE"
     gene            275..1057
                     /locus_tag="ACHKAY_004522"
     CDS             275..1057
                     /locus_tag="ACHKAY_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703076.1"
                     /translation="MENTKAIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTIMC
                     HLGFTEEVSKKHGVAIWRTMDTGMRRFITARTPGMTMMDLMQIAPLFECEPLDVFSNP
                     ALCQQLYGEMKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIKSD
                     LKRAILEASCRVVGLGKQSYVSFPAGHEGPAEILIMDADLLWQIQFLIGKSIIRAEEL
                     DQYITCTMTDEVKSVAIANARNLCRAALTELQENTTEEVESD"
     gene            complement(1087..1404)
                     /locus_tag="ACHKAY_004523"
     CDS             complement(1087..1404)
                     /locus_tag="ACHKAY_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703077.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVEGGDEDDDDDDDDFGDHVDQ
                     IVY"
     gene            complement(1394..4381)
                     /locus_tag="ACHKAY_004524"
     CDS             complement(1394..4381)
                     /locus_tag="ACHKAY_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703078.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAQSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEHLDQLLKRFSYNPQTRWQMNQRSMGSVYEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQSGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            4481..5689
                     /locus_tag="ACHKAY_004525"
     CDS             4481..5689
                     /locus_tag="ACHKAY_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5703079.1"
                     /translation="MKQQVSFKFRLKPDGQQECQMRRFAGACRFVFNRALALQNENHE
                     AGKKYIPYTKMASWLVEWKKDTETEWLIDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEISTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHFRIANIRRDYLHKVTTIISKNHAMIVIEDLKVKYMSK
                     SAAGTISHPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWHGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(5729..6094)
                     /locus_tag="ACHKAY_004526"
     CDS             complement(5729..6094)
                     /locus_tag="ACHKAY_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HFO5703080.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGVSGGSSDEEKQPSEHDGVPKLIADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            complement(6091..8010)
                     /locus_tag="ACHKAY_004527"
     CDS             complement(6091..8010)
                     /locus_tag="ACHKAY_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703081.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KMGDVAQNGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLIAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLVDQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRETIAPAGMTGGHSRAMTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAYPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            complement(8012..8614)
                     /locus_tag="ACHKAY_004528"
     CDS             complement(8012..8614)
                     /locus_tag="ACHKAY_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HFO5703082.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVRLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            complement(8601..9044)
                     /locus_tag="ACHKAY_004529"
     CDS             complement(8601..9044)
                     /locus_tag="ACHKAY_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5703083.1"
                     /translation="MIGWGVCALALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            complement(9041..9370)
                     /locus_tag="ACHKAY_004530"
     CDS             complement(9041..9370)
                     /locus_tag="ACHKAY_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5703084.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            9300..9635
                     /locus_tag="ACHKAY_004531"
                     /pseudo
     CDS             9300..9635
                     /locus_tag="ACHKAY_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         2394 a         2551 c         2237 g         2541 t
ORIGIN      
        1 tgaagcagcc gacttttctg acgcgctgat gtggctgaag gatggcaaga aagttgctcg
       61 acgcgggtgg aacggcgaaa accaattctg ctggctggtt cctgaaggac agtacccggc
      121 acgaatggaa gccattaagg gatatttccc cggcgacctc gttccgtatg gtgcttattt
      181 cgccttaaaa aatgcacaag gtgtagttgt tccgtgggtt ccttctgtag gcgacttact
      241 ggcatgtgac tggtttgtag tggagtgatt taacgtggaa aatactaaag caattcaata
      301 ccgcctgcgt aatggccaga gtgtcgaagt gaccatcaat aatgatggag tgccaggcga
      361 aaaggtttct atctctgatc tggctatcga aaaaaccatc atgtgccacc ttggctttac
      421 tgaagaagtg agcaaaaagc atggtgtagc tatctggcgc acaatggata ctggaatgcg
      481 cagattcatt actgctcgta ccccaggtat gaccatgatg gacctcatgc agattgcacc
      541 gctgtttgag tgtgaacctt tggatgtatt cagcaatcca gctctctgcc agcagttata
      601 tggtgagatg aaactcgcgg ttacccccat tgtgctgcat gaaggatcgc ttgctggcgt
      661 gtggaaagtg gagcgtattt caagctacat gcctttccat gtcaacggcg taatcactgg
      721 tgaaaatcaa cctgtttccg ttataaagtc agacctcaag cgcgcaattc ttgaagcaag
      781 ttgtcgagtt gtcggcctgg gcaaacagtc ttatgtttcc ttcccggctg gccatgaagg
      841 cccggcagaa attctgatta tggatgccga tctgctatgg caaatacagt ttctgattgg
      901 caaaagcatc atccgcgctg aagaactcga tcagtacatt acctgcacga tgacggatga
      961 agtcaaaagt gtggctatag ccaatgcccg gaacctatgt cgtgctgcat taacagaact
     1021 gcaagaaaac accacggaag aagtggaaag cgattaaaaa aaatcccgcc gattggcggg
     1081 atttcttcaa tatacgatct ggtctacatg atccccaaaa tcatcgtcgt catcgtcgtc
     1141 gtcctcatcg ccaccctcta ctgctggcca atcaacaaac cagccagcgt aaagatgcag
     1201 cgttcggaga acatcacttg cgggagcatc aagggtgtta acgaatccca tatagctatt
     1261 gggatttgcc ccagctatgg cttcagcgat catgtcctcg gtaatgtcac cggagataat
     1321 gcttaaacgc ccggaaactt cttcattatc atcaaattcg ataatggcat ctccgcctaa
     1381 tggcgctgcg attttaatct gcattattta gctcctttgc cacacctaat aacagttcca
     1441 gcaatccgtc accattcatc agtgatgcgg cagcggcctc tttgtcatga tacaactgaa
     1501 gagccataga gaatacttcc gttgctgacg ttttggaaat agtcggtgat ttctgccgaa
     1561 ttttcccggt gttacttact gaggctggcg ggtatacctt cgccatataa atattactca
     1621 atcgggatct gaagcaccat tcaggcttgc cacgcccacc gatattaacg aaagatggct
     1681 tatccccttc aacattggcc ttcaggaatg accgggcttt ctctaacaaa ccaggattac
     1741 tgtactcaag atgatggccc agctcgtgcc acagtgcact tgcattttca tcgttcaaat
     1801 tgacagcaac aacaccatta agatttgcat atgcccttcc ctggtggtga accacctttg
     1861 ataaggtcga aattttaccg ccggtcaggc gataaatatc agcaagttcc ttgcgcaggt
     1921 ctatcccacc actctgtcca gcgcgggctt cttccacttc ttccgtgata aaagagtcgg
     1981 cccactcaag agctttttct tcagatacgg atgagttggc tatcgcacta ttcatggcag
     2041 ataacacttt ctcatagacc gaacccatac ttcgctgatt catttgccag cgtgtctgcg
     2101 ggttatatga gaatcgctta agtagttggt caagatgctc aagttcttct tcgctgacat
     2161 actttttagc ctcaccaata atgccgggga gaatattgcc gttaggatta aacgctcgcg
     2221 aaaggaagag tttcagcgcc cccatgccct ccgatgcttc aatatcacca ataacacggt
     2281 taacaatggc cgcactcttc ggattagcat ccgccaacgc tctggctacg atttgcaggg
     2341 acgatacgac ctcacgctgc atatcagtcc tgatctcatc aataaactct ggcgttatgc
     2401 cgtgctcttt aaggatatcc ctgccttccg ccgttacccc atcgatatca ccgacatgtt
     2461 tattaacccg actttgcaat gccttaaatg ccttcagaat tccacgggca tcatcagctt
     2521 tactaacggc cttcctgaat gctggcaaga agtctgagtt aacctcattt tgttgatcgg
     2581 cccactgaat ggaggcttcc ttcatctcgt ccagagtcag atctcccaac gcggtatggt
     2641 ctgtgaatat gagcgataac ctctgaacca tttctgccaa tggtgatgct gattgcgccg
     2701 cgctaaggaa tgctttcacc ctggttgggc gaatggaaaa ccagtcaata gctggtggca
     2761 tatctccgtt ttttatcgcc tgcgctatct cgtcaaagcc atcgcgccca agggaggatg
     2821 cgtgatttaa caagccgcga agtaacgaat tgctgatacc gaataatcgg caccattttt
     2881 tcacgtcggc aacaggcatt cgaacaaaat gcgcaagcac ttgaacaagc tgttcatcct
     2941 ggggatctgt acgggaaagc agcctgatca gatgaataat gtctttgatg ccggatgccc
     3001 gatgtaatag caagctggta tatggagcaa caccgttgta actaccgcca gaagctgact
     3061 cgaaaagacc gccagatatc ccttgcatac cttcgttttc cagttcctga gatacttggc
     3121 gaaggatatc ctgtaacgac acatcgccgc cgccaaacat atccccgagc gcctggccct
     3181 ggtgctgtaa ctcatcattg atacgttgcg ccatcaactt aaaggcggtg gccatacgct
     3241 tcgcactacg gttattcgcg acgatgaaca acgcgagtgc tttcacttcc ggggccgttt
     3301 cgctgaacat atccccctga gcaataacat cggtaatatg ctggcctgac tccttcgatt
     3361 gccttaccag gtctaccgca tctttcaatg ccgccagcgc ctttttatcg aggctatccg
     3421 ctgtctcaat gccatcaaca atagttgtca cagcctgctt gtgcgcttct cctgataaag
     3481 cctgcatctg gacaaaatca ttggctgccg cattaagcgc cgtcagaaca ttacgcatat
     3541 ccggatcagg ttcttctgca accatcctta ccaggcgcgc atccttatat gccttggcaa
     3601 agatcgcgtt ttgtatacgg tctacaagtt gccgtgttgg tcgcccatct tccgttacaa
     3661 ggccagcagc ctgtgtggca ccaacttgcg tcatgaatcc gcgaataaac gcgtcattac
     3721 tgcggctaag cagatctcca ctttctgacg ggttaaacaa cgccatcatc gccggtgtta
     3781 tgctgtcggc atcaacaaaa gccttttcac tggctgccat ttcctggaga tcagaaatat
     3841 ttgagtcctt ggcaaactga acacggtcaa ccttcgttaa ccggcggcgc accagtaccg
     3901 gagccgtcat tgattcaacc ttttcaggcc gtatgccgaa ttcggtcgca tgttcaatca
     3961 ggtactcccg ataccgatcc gcattgccgt cctgataggc tttaatgatc cccatggtcc
     4021 gcccattacc tgactcaacg gcattgtcct caccaattat tggcgcgcca tggctggata
     4081 aaccggaatc agtaagctga gcaggccgca aatctttgga tatctggtta acctgaagaa
     4141 ggctggatgc gcgggtccgg tcgcgcggct gaagttcctg gggatagtca ggattaattt
     4201 tcccatccag agtattggat accaaaagag ctgaggcatc gacgatatca aacgctgttt
     4261 ttacctcgtc tcccttcgct gtcaccacat acgaaacccg cccataatcg ggcaggttct
     4321 ttagcagctc gatcagcgtt tctatgctgg tggccattac cacctgatcg cttaagctca
     4381 tccctgttac gccttatcca atgtgccgcc ccctacgctc ctcatagtgg ggatataagg
     4441 cacagttttc ctcccggcta tttttgctta aaatagtgac atgaaacaac aggtgtcatt
     4501 caaattccgg ttaaaaccag atggtcaaca ggagtgtcaa atgaggcgct ttgccggagc
     4561 ttgtcgtttc gttttcaacc gtgctctggc gcttcagaat gagaatcatg aggccggtaa
     4621 aaaatacatc ccttacacga aaatggcttc ttggttggtt gagtggaaaa aagacactga
     4681 aaccgaatgg cttatagatt ctccctcaca gccattgcaa cagtcactga aagaccttga
     4741 gcgggcttac aaaaacttct tccagaatcg ggctgctttt ccccgattca aaaagcgggg
     4801 acagaatgat gcatttcgct acccgcaggg cgttaagctc gatcaggaaa acagccgtat
     4861 ttttctgccg aaactgggct ggatgcgcta ccggaatagc cgtcaggtca cgggagttgt
     4921 gaaaaatgtc actgtcagcc agtcctgcgg taagtggtac atcagtattc agacagaaag
     4981 tgaaatatcc actccggttc acccttcagc atcaatgatc gggctggatg ctggcgtggc
     5041 taaactcgcc acgctgtcag atggcacagt ctttgagcct gtaaacagtt ttcagaaaaa
     5101 ccagaagaag ctggcgagac ttcagcgaca gttaagccgc aaggtcaaat tcagcaacaa
     5161 ctggcagaag cagaaacgca aaatacagcg actgcatttt cgtatcgcaa atatccgcag
     5221 agactacctt cataaagtca caacgatcat cagcaaaaac cacgcaatga ttgtcattga
     5281 ggatttgaag gttaaataca tgtcaaagtc agcggcggga acgataagtc atccgggtcg
     5341 caatgtccgg gcaaaatcag gtttaaaccg ttcgatactg gatcagggct ggtatgaaat
     5401 gcgccgccag cttgagtata agcagctctg gcatggcggt caggtactgg cagtgccgcc
     5461 agcgtacaca agccagcgtt gcgcgtgctg tggtcatacc gcgaaagaaa atcgcctgtc
     5521 acaaagtaaa ttcagatgcc aggtatgtgg atatacagcg aacgccgatg taaacggcgc
     5581 tcgtaacatt ttagcggcgg ggcacgccgt acttgcctgt ggagggatgg tgcagtcagg
     5641 ccgcccgttg aagcaggaac ccaccgaaat gattcaggcg acagcctgaa cgtagcagga
     5701 atccacgccc cgaagggcgt ggaggatgtc acgctgcctc tttaatgttg gcggctatcc
     5761 atgccgccgt gtgctgttta acttggtcca ggtcgatgta tgtgccaaca tattgactca
     5821 agtcctgtaa cgtaccgata aatgcatcag tgctctgatc gacgaattta tcagccagga
     5881 aatcagcaat cagttttggc acaccatcat gttccgaagg ttgtttttcc tcgtcactac
     5941 tgccgccgga cacgccgtac cccatctgtt gcatgatctg gtcaatttca tcgctgatat
     6001 ccagcaactc catgccactc gcggtagccg ctttggacat cagagcatcc aacttatcgc
     6061 tgagatccat taactcaata gctgatagtg tcatgccgct acccccgctt tctggattgc
     6121 taccagcaga tcagccaggt ggcgagcagc gccattaacc agctcttcgt tttcctcaaa
     6181 acgcccggca gcctgaaggg ctgcaatcgc ttcccggaca ttaccccggg cgttacggat
     6241 ctccgccatg tcagtgcttt gcatatccat cacgttattg agatattcaa tggctttatt
     6301 agcctctgca tctgcttcgc taaccgtttc atcaggctgt gccggggcag gttctggctg
     6361 agtaatctca ccgacttcgg cctgcaatgc attgatcatg ctctgcacca ttttctcggt
     6421 gccagcgccc cccggatacg caatattggg gaaagttttt tgaaactgag ttttcagcat
     6481 tacgcggaac tcgtctggtg agctggtggc cagctccaga gctttttgtg catatttgcc
     6541 aaacggacca ttagtaagtg tcttcgccag gaagtcgaaa gaatcctcgc gaggcaataa
     6601 cttcaggtcg tactcactca tttgctgatc agaaagcggg gtatcgtaag tagcaatgcc
     6661 gtagcgtgca tattcataat acgggtcacc ttcatcaggg cgcggcagaa ttgctttgtt
     6721 accttcaggt attgcgccag gggccgccgg acgcatttgc agggcatatc gatatgcacc
     6781 tacagagact tctggttcag gcgaagagct accggtatcc tccgctggtt caggttcgac
     6841 gttttccggt ttatgttctt ctggttggac caggtattcc gatacattac ccgctttata
     6901 ggctttaaac agcttgccga tcgcatctgc catgtccaca ccctgtatgg atttagcctt
     6961 gatcatgtac acgctgccat ccgaatcggt taactggata tacccttcac cgtcctcaat
     7021 gaattgcttc attgatgcac cattactgag cgtcacttcc ccgttcatat gcatacgatt
     7081 tttgatactt gcaaggcgat ccgtcatcgc gcgagagtgt ccaccagtca tccccgcagg
     7141 agcaatggtt tcgcgcccac cagttcgatt gagctgatca atctccgtct gcaaacgctc
     7201 attctcttca taaagagaat ccgcttccga tgcaacagcg ttaattttct gctccagatc
     7261 taccttctgc ccttctaccg ctgccacctg atccacgagg tcgctcatgg catcctcttt
     7321 ctggtcactg tcagcctgta gttgggttat ttcatcaaca agggcttttt tcttcttctg
     7381 cgcacgctgg aattttgccg agtttttctc tgcaaggttg gcaagtttca tggtgacctg
     7441 cgccagcgtc atatcacgtc cactcatcgg agcaacggta tgagtaacgt cttttttatt
     7501 cagtaagaac tggaaagcaa tcagcgtatc gctattggtg atccggtttt ccgctgtcgg
     7561 gctatgaaac agaatgctga tagtctgacc atcactgagc ggaataatgg ctggcagaac
     7621 cggcagcccg ttaacgttac gtgcccggcc aatttcagcc ccgccgatcg cgcgtgcacc
     7681 gttctgggcc acatccccca ttttatcact ccccgcagag attccggtac cattcagctt
     7741 ctggttcaat gcccggacaa atgcctgcat ggtccggtgt agctgcaaac gagtagaact
     7801 aatcgcctcc agtaaatccg tagcacacca gtggatcggc gtgtcataga agaacgtagc
     7861 ctcgatttct tccagggtgt tggattccgt catcagatag cggtcctcac cggccattaa
     7921 tgcgcgatat tcatcatcag tcactggcgg gggaagcacg tcaagcccag gcttgatcgt
     7981 caccccttta ttgatattga actgttccat gttaatttcc tgctttcagt tgcttaagac
     8041 ggcgtttgag ttcgccattt cgggcctttt cgttattgag tcggcctgtc tccttatcca
     8101 gcttcgcccg caaatcagtg atctgctgtt gattgaaaga caccgagttc tgcgctgatt
     8161 tataagcggc aaccacctga gcattccgct gttttgcctc ttgcaggcgc tgaaagttgg
     8221 attttactgc cggtttcttg tctaccggat tggcaacacg tttcgctttg gcgatcagtg
     8281 atttctggaa ttttgcggag tttttgcggg ccgcttgccc catgacggta ccaagcgtct
     8341 tgatatccgg cgactgagcg ttaggaatag cttttccatt cagcctcaca gacgatatat
     8401 cgccagtatc gtttacctgt atggcaagaa tttgtccgtc gttaagaacc agctttgcgg
     8461 ttttaacttt aacgccatct ttcgttgttg cgcggttgct ggagtcaacc tcaattaccg
     8521 taacaccggt tttattgatc gccgcgataa gggatttcag ccccttttca ttaacctggt
     8581 caaaatcgac cgttgcatac ttatttttcg tcatctgaca catcctgtgc gagatttatt
     8641 acgtaacttc tgcggatttg ctgagtaaca gggaaaatcc gatacaacgg gttaatgaac
     8701 gagtcgccat gcgtaaccat gacgttgaaa tgccacagcc gttctccttt acccatatat
     8761 tcagtgggta tgtacaacca ttcactgttt tcgccctgtt cagccgacgt cagacaacgt
     8821 tgttcgcctt caatcactgt cgttggcttc tgaacatcgc ggatccaata tctgaccgtt
     8881 gcgccgcgca aaaacgggaa tttagaccgg tatttgaacg gcacccggat gaaacccggt
     8941 ttaatttcca catcaccaag ttctaaatgc gtgatgtcct tgcgttttag caaatagcga
     9001 tcggctaagg ctaacgcaag agcgcataca ccccagccaa tcatttccca cctccctttt
     9061 tcaccaaact tgtaagaaca ttcagaatgc tatcgatatt cactcgtttc atccctgaaa
     9121 tcacctcatg accgttattg ctggctatcg ttaccattaa gtacgtaatt gataactccc
     9181 agccctcgtg ttgccccaat aggtacgcca ccgcgccagc tgtcactgca acaaagatct
     9241 ccgtaaccaa tcccaacaaa ttgccagact ggcgaccgtc tcggacatcc atcaggaacg
     9301 tgcctatccc accaattact gaaagcagga gcgcaatagc aactggagct aattcctgtg
     9361 tgtcaagcac aagttccctc ctacgttgtc aggaggtaat ggtatgcaaa gtaacttctc
     9421 aaccggtcat gcaaagacaa caagcggaaa aagaaaagca aagcctgcta cagctcgtca
     9481 gggataaaac tcagctctgg gactcacagt tacggctagg cattatttcc gttcagggta
     9541 aacaaaaatt aaccgagtgg attctctatg cgcagaaagt cgaatccacg gatacctcca
     9601 tccttccagt aacgtttccc gaaaagccgg aatgaggaaa ggcccggaat cgggccttaa
     9661 ttaatatcta taagctcaac aaataagagc taacaatttt gttaatttcc tctggaaata
     9721 atg
//
LOCUS       DAZFKM010000173         9641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFKM010000173 DAZFKM010000000
VERSION     DAZFKM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            146..1210
                     /gene="ulaG"
                     /locus_tag="ACHKAY_004532"
     CDS             146..1210
                     /gene="ulaG"
                     /locus_tag="ACHKAY_004532"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO5703085.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1318..2073
                     /gene="ulaR"
                     /locus_tag="ACHKAY_004533"
     CDS             1318..2073
                     /gene="ulaR"
                     /locus_tag="ACHKAY_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO5703086.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2070..2819)
                     /gene="yjfP"
                     /locus_tag="ACHKAY_004534"
     CDS             complement(2070..2819)
                     /gene="yjfP"
                     /locus_tag="ACHKAY_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO5703087.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3001..3330
                     /gene="bsmA"
                     /locus_tag="ACHKAY_004535"
     CDS             3001..3330
                     /gene="bsmA"
                     /locus_tag="ACHKAY_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO5703088.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3479..3754
                     /gene="yjfN"
                     /locus_tag="ACHKAY_004536"
     CDS             3479..3754
                     /gene="yjfN"
                     /locus_tag="ACHKAY_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO5703089.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3871..5496)
                     /gene="aidB"
                     /locus_tag="ACHKAY_004537"
     CDS             complement(3871..5496)
                     /gene="aidB"
                     /locus_tag="ACHKAY_004537"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO5703090.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5580..6743)
                     /gene="yjfC"
                     /locus_tag="ACHKAY_004538"
     CDS             complement(5580..6743)
                     /gene="yjfC"
                     /locus_tag="ACHKAY_004538"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO5703091.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6746..7384)
                     /gene="yjfM"
                     /locus_tag="ACHKAY_004539"
     CDS             complement(6746..7384)
                     /gene="yjfM"
                     /locus_tag="ACHKAY_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO5703092.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7394..7792)
                     /gene="yjfL"
                     /locus_tag="ACHKAY_004540"
     CDS             complement(7394..7792)
                     /gene="yjfL"
                     /locus_tag="ACHKAY_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO5703093.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7810..8469)
                     /gene="yjfK"
                     /locus_tag="ACHKAY_004541"
     CDS             complement(7810..8469)
                     /gene="yjfK"
                     /locus_tag="ACHKAY_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO5703094.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8520..9218)
                     /gene="yjfJ"
                     /locus_tag="ACHKAY_004542"
     CDS             complement(8520..9218)
                     /gene="yjfJ"
                     /locus_tag="ACHKAY_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO5703095.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9237..9638)
                     /gene="yjfI"
                     /locus_tag="ACHKAY_004543"
     CDS             complement(9237..9638)
                     /gene="yjfI"
                     /locus_tag="ACHKAY_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO5703096.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         2512 a         2534 c         2291 g         2304 t
ORIGIN      
        1 aaatctttcc acactcaaaa tcaacaacaa cttccctggc aaaaagtcaa aaaatggtca
       61 ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc aatcacatat tgattcgaat
      121 tgaactatca tgaaggtaaa tggcgatgag taaagtgaaa agtatcaccc gagaatcctg
      181 gatcctgagc actttcccgg agtggggtag ctggctgaat gaagaaattg aacaagagca
      241 ggtcgcccct ggcacatttg cgatgtggtg gctaggctgc accgggatct ggttgaaatc
      301 agaaggtggc accaacgttt gcgttgattt ctggtgcggc actggcaaac aaagtcacgg
      361 taacccgtta atgaaacagg gtcaccagat gcagcgcatg gctggcgtga aaaaactgca
      421 gccaaaccta cgcaccaccc cgtttgttct tgatccgttt gcgattcgcc agatcgacgc
      481 ggtactggcg actcacgatc acaacgatca tatcgacgtt aacgtcgctg ctgccgtgat
      541 gcagaactgt gctgatgacg taccattcat tggaccgaaa acctgcgttg atttgtggat
      601 tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa ccgggcgatg ttgtaaaagt
      661 aaaagacatt gaaattcatg cgcttgacgc tttcgaccgt actgcactga tcaccctgcc
      721 tgccgatcaa aaagcggctg gcgtactgcc ggacggcatg gacgatcgcg cggtgaacta
      781 tctgttcaaa acgcctggcg gctccctgta tcacagcggc gactcccact actctaacta
      841 ttatgcgaag cacggtaacg aacatcagat cgatgtggcg ttaggatcgt atggtgagaa
      901 cccgcgcggt attaccgaca aaatgaccag cgccgatatg ctgcgtatgg gtgaagccct
      961 gaatgcgaaa gtggttatcc cgttccacca cgacatctgg tcaaacttcc aggccgatcc
     1021 gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgt ctgaagtatg gcttcaagcc
     1081 gtttatctgg caggttggcg gcaaatttac ctggccgctg gataaagaca acttcgagta
     1141 ccactatccg cgcggtttcg atgattgctt cactattgaa ccggatctgc cgttcaagtc
     1201 attcctgtaa tctgtcgttt ataccggata gcgtggagaa gttcgcgcta tccggcgtag
     1261 tatttcctgg ctatttcaaa tatcatctaa aaaaatcaaa ttttatcgga atagctcatg
     1321 actgaagcac aaagacatca aatcctcctg gaaatgctcg cacaattggg ctttgtgacc
     1381 gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca ctgcgcgacg cgatatcaat
     1441 aaacttgacg aaagcggcaa actgaaaaaa gtgcgcaatg gcgcagaagc tattacccag
     1501 cagcgcccgc gctggacgcc gatgaatctg catcaggcgc agaatcacga tgaaaaagta
     1561 cgtatcgcta aagcagcctc gcagctggtt aatccgggcg aaagcgtagt catcaactgc
     1621 ggctccaccg cgtttctgct tgggcgggaa atgtgtggca agccagtgca aatcatcact
     1681 aattatctac cgctggcaaa ttacctgatc gatcaagaac atgacagcgt gatcattatg
     1741 ggtggacagt acaacaaaag tcagtccatc actttaagcc cgcagggcag cgaaaacagt
     1801 ctctatgccg ggcactggat gtttaccagc ggaaaagggc tgaccgcaga agggttgtat
     1861 aaaaccgata tgctgacagc aatggcagag cagaagatgc tgagcgtggt agggaaactg
     1921 gtggtactgg ttgatagcag taagattggc gaacgcgcgg gaatgctttt tagccgtgcc
     1981 gatcaaatcg atatgctgat caccggcaaa aatgctaacc cggaaatcct gcaacaactg
     2041 gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct ggcggaaaaa tgtcacggca
     2101 gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg gctgccatga acaggttagc
     2161 agtttatccc gtcccgtctc gcttaaggcc tgctgcaaac gtagtgattc gtcggcaggc
     2221 acaacgtcat cgtcgaggcc atgccacagc agtagaggtc tgtccccaag ttgttccagg
     2281 tggtttgtcg cttcccactc tgccagcggc gcgataatgt tattgaattc attctgctgt
     2341 gctgccgttt caggtatcag cggtggaaac agtgaacggg cgagcgatgt aaaatagccc
     2401 gatcccatca tgctggcggt acatttcacc gtggggtggc gagtggtaat tcccagtgcc
     2461 gtcatcgcgc ccatcgacgc gccaccgact gccagacggt catcaagcag ccagttttct
     2521 tcggctattg ccgcacgtaa agtagtgaat tcctgcatac tttgtagcaa gatttgccag
     2581 aattgattta accgccgcgc ttcgtcgcta ctaaaacggc taccgtgatc gggcgcatcc
     2641 ggcatgatca cccgcaaacc agcctgcgcc agcgcaacgg caaaatagct atacaccagg
     2701 ctggatgaag taaagccgtg ataaaaaatt acgcacggta acggggtatc tttttgcccg
     2761 acaggataag catgaagaac gggaatatct gccagctcgc gtgattctat ttcaatcatc
     2821 tgtctctcct ttttgtctgg tatgacatga agaaatcctt agcacaagtt tctgctggcg
     2881 gtattagttc ataatgttga gatgtgggtt acgctttcgt cacgttttca ttcgaaaatc
     2941 gcgtaccagg taacaattcg ggaacattcc cccaaagtta aattaataag gcactacact
     3001 atggttagca ggaaacgtaa tagcgttatt taccggtttg ccagtttatt attggtgttg
     3061 atgttaagtg cctgtagcgc tctgcaaggt acgccacagc cagcaccgcc agtaacgaac
     3121 catccgcaag agattcgccg cgaccagacg caagggttac agcgaatagg tagcgtaagc
     3181 acgatggttc ggggttctcc ggatgacgca ttagcagaaa tcagagcaaa agctgtcgct
     3241 gcaaaagctg attattacgt tgtcgtcatg gtggacgaaa ccatcgtgac aggacagtgg
     3301 tattcacaag ccattttgta ccgtaaataa atcagacaac ttacatagtc agctttacat
     3361 tgctttgcgt ccctatgcgt ttacctgtgc acaataaatt accggctgga agcctgggcg
     3421 tttattaccg cgatggaatg ccctgcaaca tgtggggaaa cgaaaaatgg agctgacgat
     3481 gaaacaatta cttgcctcac cctcgctgca attagtgact tatcctgcga gcgccacggc
     3541 acagtctgca gaattcgcca gtgctgactg cgtgacgggc ctgaatgaaa taggtcagat
     3601 ctcagttagc aatatttcag gggatccgca ggacgtggaa cgtatcgtgg cgttaaaggc
     3661 cgatgaacaa ggtgcgtcat ggtaccgcat tattaccatg tacgaagacc agcagcctga
     3721 taactggcgc gtacaggcga tactgtatgc atgagtgtta tatacagcaa atgaacaatg
     3781 ccggatgcgg cgcgagcgcc ttgtccggcc tacaatgttc gcatatttca ttgagttaat
     3841 cgaaaccagc gtcgcatcag tcgtatacgc ttacacacac actccccccg tcgcccgcag
     3901 caataaatca ttctggatct gctctgacaa ccgtacgccg ccgcgcgtat ctaacatcac
     3961 ctgacaccac gcctgcgcca ttggcggaga ggcatatttc aacatttgcg caccgcagcc
     4021 cagcaggaat agctgatgag taatctctcg ccccagttct tcagctggct tgcgcagctg
     4081 ctgctgtaaa cgacgaaccg cgcgatcaaa atagcgatcc tgccctttca cttccacaaa
     4141 cgcttccgac aataagtcgt atacgcccgc ttgcttattg agaacgcgca gcacatccag
     4201 gcacataata ttgccggaac cttcccaaat actgtttacc ggcatctccc ggtaaagccg
     4261 cggcagctcg ctctcctcgc aataaccaat gccgcccagc acctccatcg cttcggccac
     4321 aaacggcata cctcttttgc agatcacaaa ttttgccgca ggcgtaaata aacgcgccca
     4381 cagggcttct ttggcatcgg cacgccggtc ccacgctcgc gcaagacgaa acagcaacgc
     4441 cgtttgccct tcaagctgaa gtgccatgcg acttaagaca tgacgcataa ggggctgttg
     4501 gatcaatgga ttaccaaaaa catggcgttg atgtgcatga taaatcgcca gcgaaaatgc
     4561 acggcgcatc atggcatggc tacccagggc gcaatcaaaa cgcgtcatcc cacccatttt
     4621 caggatcaga cgaattcctt ccccttcctg ccccaacaac cagccaatgg catcctgaaa
     4681 ctccacttcg cagctggcgt tagagcgatt acccagctta tctttcagcc gctcgaggcg
     4741 aatcgcgttg cgttgcccgt caggcaaaaa gcgcggcaca aaaaagcagg acaaaccgcc
     4801 cgtagtctgc gccagcacca gatgcgcatc gctttgcggc accgagaaaa accatttatg
     4861 ccccaccagc cgataagagc catcttccag gcgctctgcg cgggtggtgt tgctcatgac
     4921 atcggaaccg ccctgctttt ccgtcattcc catgccaatc aacaaaccgc gtttttgccc
     4981 acctggcaat aagtgagaat cgtagcgatc gctcagcagc ggcgtggtcc agtcctgaaa
     5041 cggcgtgggt aacatctgta acaacaatgg cgtggcggca aaggtcatcg ttattggaca
     5101 taacgaccca gcctcaacct gcgcatgtaa cataaaacgc gccgcgcgcg ccacaaatgc
     5161 gccggagcga gcgtcttctt cccaggcaag attgtgcacc cgattggtac ataacgcctg
     5221 catcagcagg tgccaggcgg ggtgaaaacg cacatcgtcc agacggcgtc cttgcgcatc
     5281 gtagcgcaat aattcaggcg gattcacatt cgccagccgc cccagttcaa gagattcagc
     5341 cgttcctaac tgctgaccaa tactggctag aaaatcgcta tcccagccag caccttcacg
     5401 cgttaccgct tcacagagcg cgccatcaga caggtataag ttgctgttat ttaatggtat
     5461 aggttgatta aaaacggtgt gagtttgcca gtgcactgtg tctccctcca tcaatggcag
     5521 tcacccttag tatggtcact gccatggatt cctgacagaa agagattgct aaaacattct
     5581 tatccagcaa tgtaatgcgg aacgaaacgt gaggtgtctt tggtgatcag cgtgttatct
     5641 tcacggatcc ccattccgca cgcttcatca tcgacaatcc agctaccgat gagtgtgtag
     5701 ctatcgccaa atcgcggcag aggttgaaac gcctggtaga tcatcggttc atcggcgtaa
     5761 tcaccatcag cgtggtcaac gacgttattc tgaccgtcga aaatggtgac gtttccgcct
     5821 tcgcgcgagt agatcggttt acgcacatag ctttcgccag cggcaatctg cggtttttcg
     5881 ccctcaaacc aggatgcaag aaggttagga tgaccaggga agaagcgcca aagcagcggc
     5941 attagccctt tattactcaa gatacttttc cataacggct ccacccattg ctcgcgacgc
     6001 ttgcgcagca gcggaccgtt atcgtcacgc atcatccact ccagcggata cagcttaaat
     6061 gcacgctgga tgacattatc atcaagatcg gtcagtacac cgccgacgcc caaaccgaga
     6121 tcttcaatgt agataaaccg cgactcctgc cctgcctgct gggcgcagtc ctgcaagtac
     6181 agcacggtag tcctgtcttc atcggtgtcc tgacagcagc agaaataaaa cggctcccgg
     6241 ctataaagct cgctaaagcg tgaaatcagg cgttcctgaa tggcattgta ctgatcggca
     6301 tcacgcggaa taatgccgct gcgccgggca tcctccagcc acaaccactg gaaataagcc
     6361 gactcataca acgaggtcgg cgtatcggcg ttgtactcca gcagcttcac cggcgcattg
     6421 ccacaccagg caaaatccat ccggccatac agcgaaggat cgcgtgcgcg ccagctttcg
     6481 gcgatcacat cccagtacaa cggcgggatc gccagttgcg tcaggatctc ttcgtcttta
     6541 accgcgcgat caaccacctc aaggcacatc tgatgcagtt ccgcagtcgg tttttcgatc
     6601 tgctcttcaa tctggcgcag agtaaaacgg taagcccgac tctcgtccca atagatttca
     6661 ttgtcgatga tatgaaagtc gaaaccgttg ttagcggcga tctggtccag atcccgtcgc
     6721 acaggaacgt tgtgtctcag catgattagc ctccccaatg cccacgggcg ctggaagaac
     6781 gaccatagcc gccgcgcgaa acggttgacg cttttttggt ggtaaagccg cctgaagagt
     6841 aagactcttt tttgccagaa ccggagcgcc aggagtaatc accggaagtg ttgcgccaga
     6901 cggggcggga agcaaatgag gatccgccgc tgttatagac gaacggctca tcgcgatctt
     6961 tacgaataac ccggcttaac aaaaatccag aaacgaccgg gatccaactc tgttcaacat
     7021 tgtcgtagta gcaattttcg tacttagact gacaattctg ctgagtcata ttcttcggaa
     7081 catcggcata aaatgccgct ttggcgttgt tccagccacg cgcgcaaatg tcggcattat
     7141 taccgtcatc aatacaatcc tgcactgtcg aataaaacgt tccgtcgccg tcgttatcga
     7201 catcgccact atcgccacaa cctttcaata caaaaaaagc ggcaccgccc attagagcca
     7261 acgttaagta tttttgtgcg tagcgattgc gacgcggttc aaaaggatta ttcggtctac
     7321 caatgcgact gatcgctccg tgaccaattt tactgttatt tcttgatttg cgttttctgg
     7381 ccatgatcgt ccattaccat gtcatacaag ctgcgttaaa aataccgcca gccagcgcgg
     7441 cggttcccat aaacattcct gctgcggtat tgtgattaat aattttttcg cttaatgcgg
     7501 gcatataaag tctgacacca gcaaaaacga gtaactgaat caccagtgca atcccgcccc
     7561 aggcgaaata gtctggaata ctcaccgcat tgattgccgc actggataat gggataacgt
     7621 aacctaacaa tgtaccactg aacgccagtg aagccgcggt attattgttt ttgattaact
     7681 gccattcgtt gtgcggtgta attttagaat agataaaaag aaaaataatc accatagcca
     7741 cgccaataaa aaaataggcg ctaaaggcaa gaagtgaatc cagtatatgc attgtaaacg
     7801 tccttgtaat taaccaataa tatgaagtga tgtcagcggg atatcgacac ccaacgcgcg
     7861 cgaaaataac cactctggct cgccgagatc gttaaaagat tcctcaccat ttaacagcaa
     7921 atattcgtaa gtatcttcgg ttacttgccg ctggtagccc atggtaaaat tatggacctc
     7981 ccatttggca tggttttgat tttctacttt ttccagcatg tagacgggtt cgatatttcc
     8041 cggttcttcg ctgttaaaaa atcgctgcca gcgtttttcc tgccagttta agatcattgc
     8101 ccccatcact ttggcgttga tcgcctcgcg ccagtgactc tctttactga taccgtagct
     8161 ctcttcatag acaaagagct tgatatcatc aatgtcatca atatcttcgc cgccggtggt
     8221 attgatttgc agaaattcat cgcctgaagt gtagtagcgg aaaatctgac taccgccgcc
     8281 cagatcgatg tggctgacag cggctaccgt aaattcctca cccggcagcg caatcagcaa
     8341 ttcatcttcc agcaaacgaa acgctaacgt gtcgagcgta aagccactat tgagatgaag
     8401 tcccagcgga ccgcgagcga tagcaggctt attatccttg ccaaacagac gctggaaaaa
     8461 accagacata ttttatcctt aaaatagttg ccagcctttt agcgaggctg gcaaaaaaat
     8521 tactcgccct gttggcgttg cagtcttgct aatacatcct gggcgctact tttattgcta
     8581 ccgccaattc cggcttccgc cagcttttca tcaagatcgc gaccgtctgc gactttttcc
     8641 aactgtgcag cagcatccag gcgagcctga cgttcggcct gacgcgtttg caggcgtttt
     8701 aaggattctg ccgctgtcga aacgctggaa gatgcgccaa cggtagaggt tgttacagcc
     8761 tgttgtgcac gctgcatggc ttcagtggct ttaacgactt ccatttgctg ctcaaactga
     8821 gcgatacgct gcgctgtcgc tgtaactgct ttttccacgc catcacggga gacttccaga
     8881 ttcgacaaca cttgctcttc agcggtaatg agattctcaa ggcgcgcgat ttcttcagca
     8941 acttcgttaa tcaacgacgg attaacgttc ttgcttaacg cttccagcgc acgggcttcc
     9001 agactggctt tgcgctcacg taaatctttc agcttatcgt gacttaattt cacccgcgcc
     9061 agcagatcaa cgcgagattt tccggcttta tcgagttcag ctttagcgtc acgaatatgc
     9121 tgttccagca tgcgcacgcc ctgggtttct tcaatggatt cttccgcctg ggagataaac
     9181 gatttcccca gcgtaaataa actttttaaa attcccataa atacccgtcc ttgtaattag
     9241 tgtgaatatt cttctgtaat ttcagccaga tccaatgcgt tatctaccag cgaggttatc
     9301 tcaagcagga tatcttcaag agaagattta agcgataacg ccccgaaaac aatgtaatac
     9361 tcttcctgtt gcacgctgga gattcctacc gatgacaacg gcatcatctt ctgatttctt
     9421 aataagaagg tattaaattc atctggattg ctgatactac ttaccggaca aataaaggtt
     9481 tcgataatca tttggcggga agtaaaaaga atattgattt gcaaatcgcc ataatcattc
     9541 attttaataa ttaatgcgct ctcgctattt gtcacatcaa tattttgttc aggtacagtt
     9601 tgcagcgccg tcgccagcgc caacggattc catgtcatat a
//
LOCUS       DAZFKM010000174         9551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFKM010000174 DAZFKM010000000
VERSION     DAZFKM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..146)
                     /locus_tag="ACHKAY_004544"
     CDS             complement(<1..146)
                     /locus_tag="ACHKAY_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703097.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSL"
     gene            224..1318
                     /gene="ddlA"
                     /locus_tag="ACHKAY_004545"
     CDS             224..1318
                     /gene="ddlA"
                     /locus_tag="ACHKAY_004545"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO5703098.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1342..1554)
                     /gene="yaiZ"
                     /locus_tag="ACHKAY_004546"
     CDS             complement(1342..1554)
                     /gene="yaiZ"
                     /locus_tag="ACHKAY_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO5703099.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1814..2122
                     /gene="yaiY"
                     /locus_tag="ACHKAY_004547"
     CDS             1814..2122
                     /gene="yaiY"
                     /locus_tag="ACHKAY_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO5703100.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHKAY_004548"
     CDS             complement(2181..3275)
                     /gene="yaiW"
                     /locus_tag="ACHKAY_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO5703101.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHKAY_004549"
     CDS             complement(3288..4508)
                     /gene="sbmA"
                     /locus_tag="ACHKAY_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO5703102.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4860..6017
                     /gene="ampH"
                     /locus_tag="ACHKAY_004550"
     CDS             4860..6017
                     /gene="ampH"
                     /locus_tag="ACHKAY_004550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO5703103.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6018..6641)
                     /gene="iprA"
                     /locus_tag="ACHKAY_004551"
     CDS             complement(6018..6641)
                     /gene="iprA"
                     /locus_tag="ACHKAY_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO5703104.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6729..9426)
                     /locus_tag="ACHKAY_004552"
                     /pseudo
     CDS             complement(6729..9426)
                     /locus_tag="ACHKAY_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9456..>9551)
                     /locus_tag="ACHKAY_004553"
     CDS             complement(9456..>9551)
                     /locus_tag="ACHKAY_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5703105.1"
                     /translation="FNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT         2493 a         2385 c         2245 g         2428 t
ORIGIN      
        1 agagaaaaaa actcaccgca tggccattta agccaggaat aaattagctt cccgttcatc
       61 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
      121 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
      181 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
      241 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
      301 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
      361 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
      421 tattgcgtta cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
      481 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
      541 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
      601 gttcgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg ttaccaaacg
      661 tctgctgcgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgccaatcg
      721 tcacaacatt agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
      781 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
      841 tgccgtcgat ctggcgttcg aattcgatca taaagtgatc gttgagcaag ggatcaaagg
      901 tcgtgagatc gaatgcgcag ttctgggcaa cgacaacccg caagccagca cctgtggcga
      961 gatcgtactc accagcgact tctatgccta cgacaccaag tacattgacg aagatggcgc
     1021 gaaagtggta gttccggcag ccattgcgcc agaaatcaac gataagatcc gggcgattgc
     1081 cgttcaggct tatcaaacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     1141 cccagaaaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     1201 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     1261 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     1321 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     1381 aatcagcccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     1441 ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc
     1501 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     1561 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     1621 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     1681 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     1741 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     1801 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     1861 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     1921 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     1981 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     2041 cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca
     2101 ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct
     2161 ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     2221 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     2281 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     2341 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     2401 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     2461 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     2521 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     2581 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     2641 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     2701 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     2761 cgcacaggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     2821 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     2881 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     2941 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     3001 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     3061 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     3121 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     3181 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     3241 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     3301 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     3361 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     3421 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     3481 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     3541 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     3601 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     3661 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     3721 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     3781 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     3841 agcaccggca gaaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     3901 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     3961 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     4021 acatagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     4081 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     4141 agcgcggttt gaatcagatc atagaacggt gcataccagg cgttgacggc gaccccgact
     4201 tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc
     4261 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     4321 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     4381 ccggaagcgc cggtgatacg cgccacccag tcacccccac cggcttgcca gaagataacg
     4441 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     4501 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     4561 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     4621 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     4681 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     4741 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     4801 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     4861 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     4921 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     4981 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     5041 gcgtctttcg cagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     5101 cggtcgtgcg tattgcttcc ctcaccaagc taatgaccag tgaaatgctg gtgaaattgc
     5161 tcgaccaggg gaccgtgaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     5221 gcgtgccaac ctacaatggg acgcccatta cgctggtcaa tctggcaacc cataccagcg
     5281 cgctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     5341 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     5401 cggcgtactc taaccttgcg ttcgacctgc tggctgatgc actggcgaat gcgtccggca
     5461 agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca
     5521 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgcagagcgc ggtgccagtc
     5581 catgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     5641 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     5701 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     5761 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggtcccg aaagagggtc
     5821 gtccggggat tattcagaag acaggaggtg gtggcggttt cattacctat atggcgatga
     5881 tcccgcagaa gaatattggc gcgtttgtgg ttgtcactcg ttcgccgttg acgcgcttta
     5941 aaaatatgag cgatggcatc aatgacctgg tgaccgagct aagcgggaat aaaccgctgg
     6001 ttatccccgc gtcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     6061 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     6121 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     6181 tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca
     6241 atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg
     6301 tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat
     6361 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     6421 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     6481 agagagataa ctccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     6541 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     6601 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     6661 atgcccgtca aatccttgac gggcattatt tagattaaat taccagaatt tcttcggagt
     6721 gaagaatatt accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     6781 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     6841 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     6901 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     6961 gcctggtctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     7021 ctgtcgtaag actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     7081 tggaacagac cagaaacgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     7141 tcgtaaccgg ctttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     7201 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagctacca
     7261 tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct
     7321 tgatccacct gaccagaacg gtcattcatg tcacctttag cgaagcctgc agccgcaccg
     7381 acgatccact tagcgttgtt accgtcaatt ttggtatcaa caccgaccat gatgccgtta
     7441 acatcctgat catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt
     7501 acccatgcgc cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg
     7561 gtgtcttgtt ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta
     7621 gcgtagtcgg tcagctccat ctgttgcaga acaacggtgt taccgcgctg ttcagcctga
     7681 taggtgtatg cacccaggtc agctttgtta gcagcagaga aggtcgcgtt ggtgttgacg
     7741 tcgtttacgt agataatttc tttatctttg taatcagcga tagcgccagc gcctgttgcg
     7801 ttgtcgatac gaactttgta gttaccggtt gcagcaacaa cgttgtcaac agagctgtta
     7861 tccagttcgt cgccgttgtt catgtcaccg ttaccgttga tagtcagatg accatcagag
     7921 ttcatagcaa caataccgta gccgtagtta gacttggaag tatcccaggt acggtcgttc
     7981 accaaatcag cgttcagaac ataatcgctg ctgtggatat cgaatacacc agacactacg
     8041 ttgccgttat ggtcagcgat gttgccgctg gtcagttcca aagtatcagt ctggaacgga
     8101 ttatcatcga actggtctac attaatatcc agtaaaccac cgttagtcac agttattgta
     8161 tttgctaaca gcgacggagt gtaataacca ttatagatac ttgaattgtc atcactagac
     8221 aacgctacag tactgtagct atcaactgtc agatgatcag ttccaacata accttcatct
     8281 ccaatgttca aagaagaacc attggtcaga gcgatgctat cagaaatcaa gctagattca
     8341 gacacattaa cctgagagcc attattcaca gctaaagtat caatgtttga agcgttaacg
     8401 gtattccatt cagaaccatt attcagagtg acgttaaaca caccgctctg gaatacttga
     8461 tcatcaatcg tacgacccca agcatttgct tctgttacaa cgcccggata caggctatta
     8521 cctgtaacat cataccagtc agtcgaagca gtagattcat catcaacatt tgctgatgta
     8581 gtagctgagc ctacccactt agaaccatta tccagagtga tattcagttc atcaacatta
     8641 agtgagtcat cagcccagcc accattggtg tccaagtacg ccatcggcat tactgtcatg
     8701 accttgagga tagaagtttg cgttgaaagt acttgcgaaa acaacatcac ccagcagagt
     8761 agaattgctg aaataaacat tgtttttcat ggagtaattt gaagccgcac tatcactgaa
     8821 atacagagct acatcattag cttcgccgtt accagagtaa tcactaggtt ccgcagagtt
     8881 accatagaaa ccactgtctt ccagagtagt tacagaacct gaagtcactg tggaatcggt
     8941 aactgagata ttgttatccc aagtatgaga ttcagtagtg taagtattgt ttacggtatt
     9001 accctgagtc aggctgatac cagcaacgcg ggagttgtta gaaatattga tgttactttc
     9061 gacatcaaga ttcactgcca gcccaaggcc aacaccaccg tttgcaaaca aaccggcgta
     9121 ttcttcacct tcaccagctg gggtatggaa ggtttcgtta gtatatttgg taacgtcacc
     9181 gtccagatat gaatcagtga agtataaacc ttcataatcg tcatcaattg tagaattagc
     9241 aatatttaaa gtgaatacat caccatcatg ccaattatca tcaattacac gatcagtacc
     9301 gtattcagag aactgataga cataatgacc atcactgctt tcacccatag gcagcattga
     9361 agtgattgaa ccatgaatca gggagttgct gatgttcaac tggtttgttg ttccatcagt
     9421 aaagttgatc ctacccacgt aatatggaca caggcctaag cgaggttctt gttttcaaat
     9481 tgttccggac tgaggccgcc acacaaactg tgccgccgcc accgattgta atcacattcg
     9541 atataattaa a
//
LOCUS       DAZFKM010000175         9453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFKM010000175 DAZFKM010000000
VERSION     DAZFKM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(125..1612)
                     /gene="yeeO"
                     /locus_tag="ACHKAY_004554"
     CDS             complement(125..1612)
                     /gene="yeeO"
                     /locus_tag="ACHKAY_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO5703106.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1773..1848
                     /locus_tag="ACHKAY_004555"
     tRNA            1773..1848
                     /locus_tag="ACHKAY_004555"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1806..1808,aa:Asn,seq:gtt)
     gene            complement(1886..2836)
                     /gene="cbl"
                     /locus_tag="ACHKAY_004556"
                     /pseudo
     CDS             complement(1886..2836)
                     /gene="cbl"
                     /locus_tag="ACHKAY_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(2938..3855)
                     /gene="nac"
                     /locus_tag="ACHKAY_004557"
     CDS             complement(2938..3855)
                     /gene="nac"
                     /locus_tag="ACHKAY_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO5703107.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4181..4256
                     /locus_tag="ACHKAY_004558"
     tRNA            4181..4256
                     /locus_tag="ACHKAY_004558"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4214..4216,aa:Asn,seq:gtt)
     gene            complement(4300..5247)
                     /gene="ldtA"
                     /locus_tag="ACHKAY_004559"
     CDS             complement(4300..5247)
                     /gene="ldtA"
                     /locus_tag="ACHKAY_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO5703108.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(5309..6388)
                     /gene="cobT"
                     /locus_tag="ACHKAY_004560"
     CDS             complement(5309..6388)
                     /gene="cobT"
                     /locus_tag="ACHKAY_004560"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO5703109.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6400..7143)
                     /gene="cobS"
                     /locus_tag="ACHKAY_004561"
     CDS             complement(6400..7143)
                     /gene="cobS"
                     /locus_tag="ACHKAY_004561"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO5703110.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7140..7685)
                     /gene="cobU"
                     /locus_tag="ACHKAY_004562"
     CDS             complement(7140..7685)
                     /gene="cobU"
                     /locus_tag="ACHKAY_004562"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO5703111.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7837..8010)
                     /locus_tag="ACHKAY_004563"
                     /pseudo
     CDS             complement(7837..8010)
                     /locus_tag="ACHKAY_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7989..8177)
                     /locus_tag="ACHKAY_004564"
                     /pseudo
     CDS             complement(7989..8177)
                     /locus_tag="ACHKAY_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9174..9453)
                     /locus_tag="ACHKAY_004565"
                     /pseudo
     CDS             complement(9174..9453)
                     /locus_tag="ACHKAY_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2597 a         2534 c         2015 g         2307 t
ORIGIN      
        1 ttctaccgac tgaactacag aggaatcgtg agaacgaggc gaatattagc gatgcccacc
       61 cacaatgtca aagcctgttt tttaaatttg aaatcgtttg ctgaaataat ctgcattttg
      121 ttgtttattc cgacacaact ggcttttttt cacacttttg cggttcgggt cgagggtatt
      181 tccatagcca acgtccagta accattcgcc agtaaaacag cacggcccgc acagcccagt
      241 cggcaaacat tcccatccag acaccaacca caccccagcc aagcatgatg cccagcacat
      301 aaccgacgac aacccggcaa ccccacatgc tcaacataga aacccacata gcgtaacggg
      361 cgtcacgagc ccccttaaat ccagcgggta gcacccatga ggcggaccaa ataggcataa
      421 ataaagcatt tagccaaatc agaatcacaa cgacatgttt aacctgtgga tcctgggtgt
      481 aaaacgatgc cataaccccg gcaaagggag ccgttagcca ggcgatggcc gttaatccaa
      541 gagtggacag ccagaagaca tgccgcaact gaatctctgc ttgcgctatc tgccctaccc
      601 ctaaccttcg gcctgtaatg atcgtagaag cagagccgag cgcacttccg ggtaagttga
      661 taagagccgc aattgaaaac gcgataaaat ttccggcaat aacactggtc cccatcccgg
      721 caacgaacat ttgggttaat aaccgaccac tggtaaataa cactgattcg acactcgctg
      781 gaataccaat ccccatgact tcccagataa tgctaaaatt cagcggttta aaatagctct
      841 ttaacgaaat ccttagcgca ggattaaaac caatcgccag cacccacaaa attgcaactg
      901 cgccaatata acgagaaatg gttaaaccca acccagcccc gacaaatccc agtcccggcc
      961 aggagaaaag gccgtaaatc aatatgccgc taataataat attaagaata ttcaggctac
     1021 cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaatca
     1081 aagtgatggc aactgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg
     1141 ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat
     1201 gaataagcgt tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg
     1261 ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg acagtagtac
     1321 caagatcgat agcagcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca
     1381 cgccggccat cgcatctttt cccagccagc tgaccagaaa agtgctcagg acacccatca
     1441 acaggacaca ggcattctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga
     1501 acaacacttt gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta
     1561 aagcagagga gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata
     1621 atgagggaga atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac
     1681 aaactgttga tagaaacggc aaacagttgg gaaatttaaa aatcgggttt gacaaaagat
     1741 ttttcgccgt taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc
     1801 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa
     1861 gcccgctttt atagcgggct ttttgctata tctgataatc aatttcctct tcactgttct
     1921 ccatcacctg gcgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc
     1981 gccagacata gttacgttga agttgccctc gcttcagtcc caaccataca gtattggcat
     2041 cgaaaagatg ccgcgtatcc agacggatta aattctcttc ctcttgttcg ccgctggatt
     2101 gttcggcaac taatccaatc cccagcccaa gagcaacata ggttttaatg acatcagaat
     2161 cctgcgcact taatacaata tctgccagca aacctttgcg ggcaaatgcg tcatcaatac
     2221 gtgagcaccc cgtaatcccc tatcggtaag tgattaacgg ccacttcgct attgattcca
     2281 gcgtcaacgg tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa ctatggtgcc
     2341 aacgaaacca cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac
     2401 caatatcagc ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta ccctggatta
     2461 gctccagccg aacctccggg aaaagttcgc gaaaagcttt aatgacctca ggcaagctat
     2521 aacgtgcctg agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca
     2581 ggtctgcaag ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta
     2641 atgctttgcc cggttcagtc atacccagca gtcgcttacc tcgtcgaaca aatatttcga
     2701 tgccaagttc atcccccagt tcccgaatat gacggctgac gccggactgt gaggtaaaaa
     2761 gcatattcgc aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta
     2821 gttgttggaa attcacggta aactccgggc agctcagatt tcccgttatt gttaaagtct
     2881 aatgcccggc ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta
     2941 gctcaccaat tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa
     3001 ctctttcact gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa
     3061 agagagactc atggaaggcg tggtaatgcg tgacatccac ccatttactg cgccacataa
     3121 cgaacgcgcg gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt
     3181 aagcgtggca atagactcaa tttcaccaat gacttttgcc gtgagccgcc gtagggaaaa
     3241 agcctcatca acacgaagtc taacagcact gtaatcactg gggagaaaga ggttcatttg
     3301 cgcaatagca ttaacatcaa cgctttgccc cgggcaatct tgagttccta ccaggaaaag
     3361 atcttctttc agcaaagcct gactggatac cccagccaca ggggaatgct cataaatcac
     3421 cgccatatcg agttggtgat ttatcagttt ttcgttaagc actgcaccgc tattttcatg
     3481 aagatagata acgatctccg ggaattcagc gcgaaccgct tgtaataagg gcatggtgat
     3541 agatgacgca gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgcctgacc
     3601 aacgttatgc accgccagtt gggcctgctc acactgacgt aaaatggccc gcgcatgggt
     3661 atagagaatt tttccggcgt ctgttggcgt aacgccccgt tttgtgcgaa tcaaaagttg
     3721 ttgatttaac tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg
     3781 caatacttca gcagcctgag tcaggctacc aatatctaca atttttacga agtatttcag
     3841 gcgtctgaag ttcatgttgc ctccggtttt tgagaatcgg cccaagtgcc gccattactt
     3901 acaaccagac tgcaagatgc ttgccagttt tattttggcg ttgatgtaca agctaaccaa
     3961 ctgtcaaata agagattatg atagattcgt cattggctct tttcaccagc agtcgcgttc
     4021 ccctgcccta taaaaggagg gtatgcacca cgatggttca ttaccaataa gattgaaatc
     4081 tcatcacttt gttgaaattg gcagcaaaca aacaaaaaaa tgcatttcac cctttgacat
     4141 caccatgcac tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt
     4201 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     4261 caaaaaagcc tgctttctag caggattttt gctttctaat taccaacgct cataaaacat
     4321 ctgtcttgaa ccagaactaa tttgcacagg cattcccgat cgacgttgca acgcagcatt
     4381 tgcgcgattc acatcaactt cttgcccgtt gataaacgcc cgcaaagatg gggttaccgg
     4441 caatggcact tttcggtcag actcatattc tgcacgattg cgcgacagcg gctcatgaac
     4501 ttccagccag ttcgagccat ctggttcagt ggtgtatttt actggctggt cgataatttg
     4561 cacacgcgtt ccaacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg
     4621 aatacagccc tgacttaccc gaagtccaat accaaaattg gcattggtac cgtggatggc
     4681 atacaacctg ccaatataaa tcgcgtacag ccccatggga ttatcgggcc ccgcaggaac
     4741 aaatgcgggc aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt
     4801 ccaggttgga gcttcttgtt tacgttcaac ggtagtcacc cagttacgcg gggtttctcg
     4861 cccagcctgg ccgataccaa taggaaagac ttccacagta ttactgtctg gcgggtagta
     4921 ataaagacgc atctcagcga cgttaaccac aatcccttta cgaacagtgg cgggcaaaat
     4981 cagttgctgc ggaatggtga gttgcgagcc agacttcggc aagaaaacat cagcgcccgg
     5041 attcgcttcc agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg
     5101 ctgggtattg tgatcaggaa cagttacagt aagcgactgc cccaccaaac ggctaccctc
     5161 tggaggtaat ggataagtta ccgccaggct agtatggctg gcaaaaagca gagcaaatga
     5221 gcaaagaata tttacacgac gcatcatgtc cctttcctat gtcgcgaaag ctatccgtta
     5281 agtatagctt ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg
     5341 gtagaacaat attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt
     5401 cgatgatggg catcgccaga gctgcgccac tcccctcacc taaacgcatc tccatattga
     5461 gataaggctc cagccccaaa tgcgacagcg ctatacgcgc ccctttttct gccgacaggt
     5521 gagaaggaat gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcagcag
     5581 cataagaaag aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca
     5641 cgccagccat tccgaccaaa tcaaatcccc ccacttttgc cagaacatca acgccatcct
     5701 gcggatttgg ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt
     5761 tatctgtcgg caggtttgcg ccaatcccaa ccacttcttc aggatcccgg ccagtgattg
     5821 tgctgactat tgccgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca
     5881 gcgtgacacc gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt
     5941 cagcctgacg gcgactcatt gccggagctg aagcaatatt gccgctacct cgtgcgacac
     6001 gcatgttgat aagcccgggg ataggctcag cactatcaat accaacatca actacgtgga
     6061 cgttagcgcc cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt
     6121 cagcctgaat ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt
     6181 gatcggcaca cataaccagt accgcttttt tgcccacatg cggtatgcca ttcaaccccg
     6241 gcattcccgc cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga
     6301 gtaacccgtc aatatgccgt tgtgcacgcg acatagcggc aggatcgata gcagggatcg
     6361 tattcagtaa atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa
     6421 gaccagttca ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt
     6481 acgtttgaga agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac
     6541 accatgcata cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca
     6601 ggtttgtcgc ccatcaattt tgccaatgaa cacattgcca agcccttctt cccgggcata
     6661 acgatgacga tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag
     6721 cgatgcaagg atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag
     6781 tacaacaaaa atcaatgcca gaccgccatg ggtgcctaaa cggctatcac gcatgatttc
     6841 caacatgcga tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc
     6901 taggtggaat cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg
     6961 tacgccacac catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa
     7021 tccaattaaa ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga
     7081 cctgcgacgc ggtacaggca gacgcgtaat aaatgagagc atcgcccaaa ataatttact
     7141 catttgattt ttactccaat acccgaaacc accagccata cttcatttgc cgcagcggcc
     7201 aactgctgat ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc
     7261 acaatcccca ttcccacttc gttagtcact aatacaacct ttgcggggca acgttggcag
     7321 gcagcaatca acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat
     7381 tcatcagggt ctttatcgcc gccataatca aacaacagat tagtcaccat tgtggtaacg
     7441 cattcaagca acacaacctc attcgggtta atgtctgcat gaattaattc atcaagatgt
     7501 tgccagcgct ccactgtgcg ccagtgctcc gggcggcctt gccgatgatg ttctatccgt
     7561 gcagccatct catcatcaag gatttgcgag gtagcgatat acagaacctg tgaagagtcc
     7621 ccaataagcg cctctgcgtg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc
     7681 atcataccga ctcctgattc aaatgtatga tggtattcat tgaacagaac aatggatccc
     7741 ccaccgttgc tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa
     7801 tccagcattc tcaatccatt acgaaataag atggcattac ggaatagcca gactttctcg
     7861 tgttaacgtc cgacaaaatg cgtcattgac agcggtttct gtggattgac acatttcatt
     7921 cagataatga attaatgcta ctgccggaac aaccagcaaa cgggcattgt gttctgaaat
     7981 ccaggctatt gattcaacgt cagcgacaga tctgcgctgt aaaagaactt gtaaaacaac
     8041 gtaatagaat tatccggaat ggtggcgact atgcactaga acaccacaat ttcgctctct
     8101 cggtaaggca ctgtcacagc attcaacaga atgtgacttg ccagatttat tagcgccacc
     8161 agatgtttaa ccgggtaacc acgacccagt cggggatata ttccatcttc tcgctgacat
     8221 catcaagaat aaaaaggttt atcacactca gagcaacgtt cttcagcagg cgggataatt
     8281 cttcaacatt tagaagatgt gttattaaag gcatacaaac tggacgtatt gtattttctt
     8341 ttggtggttg acctaaggta gcagtttatc ctgatgcgct gagatttctg taatatctac
     8401 gtcaacattc tcctcgaata gaaaatgcag ccccggcaag tgattcacat tttttaacaa
     8461 aacgttgtta gcgtgccatt ttcagaagct ctccaagaaa gcgaattctt ttctcaaatt
     8521 ctgcattctc agacacagct ttctactcca tggtaatggc ccaaatacga agttgctcag
     8581 gatcattagt ggtgctgaga agttaaataa ccatgcggag taccactcgt catcacgtat
     8641 ttatatatca tgaagctatt gttgtgtttt gtacatcagt agatatattc tgatatactc
     8701 cttttgctag acataacctt tcacctgctt gcaaagcttc tgtgttctga cattgccaaa
     8761 ttgttgcaat tctgtatcca gccttctttc agtcatagct tcgggccgcg ataagactca
     8821 ctgatctgac cctgattcct cttgcagact ttatagacca attaaaatgc agtttctgca
     8881 ggtcaacgtc tgaccatcat tgtcatcact ctggccatta gagtaacctt ctgcattcat
     8941 ccttttgtaa aaagtttatg tcagtatcag caattaaccg gacctgatac tgatatgagt
     9001 cttaccgcat atacggtcaa tttcagcaat taattacatt atccacgcca aagtgtttgt
     9061 catcgcaatg atggtacctt ctttcagaca ccattttttc aactccgttt tccacggacc
     9121 gcactcttat gtcaagagtg cggtgatcct acccacgtaa tatggacaca ggcctaagcg
     9181 aggttctggt tttcaaattg ttccggactg aggccgccac accaactgtg ccgccgccac
     9241 cgattgtaat cacattcgat ataattaaac accgttgccc gcattatttc ccggctgata
     9301 aagtgttctc catggataca ttccactttc agcgaatgaa agaagctttc cacgcagatt
     9361 atgccgcttc agttgcgcct gataatctgc tgaacagtac tggcctccac ggtccgtgtg
     9421 aacgataacg ttccggggcc tcttacgccg cca
//
LOCUS       DAZFKM010000176         8789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFKM010000176 DAZFKM010000000
VERSION     DAZFKM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..278
                     /locus_tag="ACHKAY_004566"
     CDS             45..278
                     /locus_tag="ACHKAY_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703112.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(256..663)
                     /locus_tag="ACHKAY_004567"
     CDS             complement(256..663)
                     /locus_tag="ACHKAY_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703113.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(686..904)
                     /gene="ydfC"
                     /locus_tag="ACHKAY_004568"
     CDS             complement(686..904)
                     /gene="ydfC"
                     /locus_tag="ACHKAY_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5703114.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(977..1276)
                     /locus_tag="ACHKAY_004569"
     CDS             complement(977..1276)
                     /locus_tag="ACHKAY_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703115.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(1541..1948)
                     /locus_tag="ACHKAY_004570"
     CDS             complement(1541..1948)
                     /locus_tag="ACHKAY_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703116.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2025..2252
                     /locus_tag="ACHKAY_004571"
     CDS             2025..2252
                     /locus_tag="ACHKAY_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5703117.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            2236..2787
                     /locus_tag="ACHKAY_004572"
     CDS             2236..2787
                     /locus_tag="ACHKAY_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5703118.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            2759..3799
                     /locus_tag="ACHKAY_004573"
     CDS             2759..3799
                     /locus_tag="ACHKAY_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO5703119.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            3792..4253
                     /locus_tag="ACHKAY_004574"
     CDS             3792..4253
                     /locus_tag="ACHKAY_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5703120.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            4287..5020
                     /locus_tag="ACHKAY_004575"
                     /pseudo
     CDS             4287..5020
                     /locus_tag="ACHKAY_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            5017..5181
                     /locus_tag="ACHKAY_004576"
     CDS             5017..5181
                     /locus_tag="ACHKAY_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703121.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            5186..5719
                     /locus_tag="ACHKAY_004577"
                     /pseudo
     CDS             5186..5719
                     /locus_tag="ACHKAY_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            5880..6638
                     /gene="paa"
                     /locus_tag="ACHKAY_004578"
     CDS             5880..6638
                     /gene="paa"
                     /locus_tag="ACHKAY_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO5703122.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            6974..7129
                     /gene="hokD"
                     /locus_tag="ACHKAY_004579"
     CDS             6974..7129
                     /gene="hokD"
                     /locus_tag="ACHKAY_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5703123.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            7350..7607
                     /locus_tag="ACHKAY_004580"
     CDS             7350..7607
                     /locus_tag="ACHKAY_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703124.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            7677..7955
                     /locus_tag="ACHKAY_004581"
     CDS             7677..7955
                     /locus_tag="ACHKAY_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703125.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            7957..>8789
                     /locus_tag="ACHKAY_004582"
     CDS             7957..>8789
                     /locus_tag="ACHKAY_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5703126.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     T"
BASE COUNT         2375 a         1949 c         2294 g         2171 t
ORIGIN      
        1 tcacccgtgg agaactcctt aattaccaac cctcaggagg gtgaatgtta aaatcaactc
       61 ttattgctaa atgcctttat caaaatcgca tggtaagcag catttcaata ggcgagtctg
      121 cagttaaaag tattttcgaa gagtactttc ccgggcatga ttttaataaa tggaatacca
      181 aattaccgcc agcagtttca acgcgtattc tgaaagcaac cgaaagagca agtacaattc
      241 gcgttaacta tttcattaaa gatttgtggg atctttgata tccacagagc ctaaagtatg
      301 tgcatatgga tgtgctatta tgcgccctcg cagatttgca tcattttcta aattcactga
      361 acgaaacagg gcatcaacaa ggctctgtac aatgcaaagg caatcgaaga ctgtcgccgt
      421 ttctgttttg attgatgaaa gaacatggcc attcacgcaa acagaaatta cccgtttatt
      481 aacatcgctt tcctgctttt gattatcaga accatatagc ccagaaaaag cattgcgcac
      541 attacgaacc atattatcga tggtttcttt ttctgcggta ctaaggtcaa gagtagccag
      601 ttgtgaacga actatattcg atgccatttc ctgtaatggc gttggtaaat ctttaaattc
      661 cattattagc ctcgttggtt agctattaac gtgggtatgt aatcattctg gcaatgctta
      721 atgccgctgc tttttccaga ttggtgatat cctgctccag agcggacaga ttttcagcct
      781 gcttagccct ggcttcattg gcccatttca ggtcctgcac cgccttaatt ttctggtgca
      841 tccactcata aagttcatca tcggtatagt ctggcgcgat gatgacgggg tctcgtttct
      901 gcatgtcggc tccttgtggt tagcgttgcc tgctttttaa ccacgtcagg cgaggtggta
      961 tcctctgagg ggtctgttac tcgagaggaa attggttatg aatacaatca agttttcttg
     1021 cccagaatgt ggtggctaag tctttgacac atcctttaag ccgcagggct ctgacagttt
     1081 cgcgggagcc atctgcaaaa attgtggtca ccttgtaact gaagatgagt cctcgcagtt
     1141 cgatgacgaa atcgttgaca atatcttcgg tgcactcacc agagactttc tgaagtaaag
     1201 gcgcataccg cttagttacc gctctgataa ctcttacctg tccggcaatg gcgctgatat
     1261 caatataaag cgccatcgct gtttctttgc tgatccctgg acgccttcca ttctgatgtt
     1321 tgacttcgcc cactgagaaa tcctctgctt ccccttaacg ccgggtagcg gaactgtttg
     1381 ctgagaacac cgtgcggtgt cttgatgagt agaatttaga atagcctaag agttatggtc
     1441 aagctttttg tgtagaaaaa cctaagcttc ttgatgtaaa aaacacaagt atttgaaagt
     1501 ttgtgctttt tattacagag agttgcgaaa aaaagggggg ttatttattt gcgcttcttt
     1561 tgcgagcttt gagtagttct tcaaaaagtt tgttgaaatt ctcaactcga gcacgcatct
     1621 ctgacaacag agccttttgc tctgactcag gcagtgcgtc gaacagttga agcaactctt
     1681 tttgatcttc tgtcagattg actggctgat tatctgggat cggttcgcct ggttgcttat
     1741 cttcatctcc aaaaagaagc caagtcggcg agcactgaag cgcctggctc agtgcgaata
     1801 atctcttccc cgctggctgt gtttcatctc tttcccattg agaaattgtt acgtgagcca
     1861 ctttgaccag cttacctaat gcggcctgag acagttttaa tttttttcgc ctgtataaga
     1921 ggcgagcacc gaaggtttcg tttttcatat taggtaattc taatttttct tgacttaggt
     1981 ttctctacga tctagtttcc ttaggaaaat ctaaggggtt cgatatgttg aaaattgatg
     2041 ctatagcgtt ttttggcagc aaaacaaagc ttgccaatgt cgcaggagtt aggctggcaa
     2101 gcgttgctgc atggggggaa ctggttcctg aaggtcgcgc gatgcgcctg caagaggcat
     2161 ccggcgggga acttcagtac gaccccaaag tttatgacga atatcgtaag gcaaagcggg
     2221 cggggcggtt gaacaatgaa aatcaccact gaacaggttt gtgaggctct ggatacctgg
     2281 gtatgccgac caggaatgac acaggagcag gcgacgatat taatcacgga agcattctgg
     2341 gctctgaaag aacgcccgaa catcgatgtt caacgcgtca cgtttaatga tggcgaggtt
     2401 gatcaacggg cgctgggcgt taaccgggtg aagatattcg aacgctggaa agctatcgac
     2461 accagagata agcgtgacaa attcacggcg ctgattccgg caattatgga ggctatccgg
     2521 atcagcgatt tcagattgta ttgtgaaatt actgacggaa aaagcattac gtacatgatc
     2581 gccgggttaa acaaagaata tggcgatgtg gtggagtccg ggctgctttt tgcggatcca
     2641 gttgttgtgg aacgtgagac tgacgagctt atagaaaaag ctattgcttt caagcacgcg
     2701 tatcgtcagc aataccaata ttactttgca gataaacaaa tgtctgccag gggttcgtat
     2761 gagtatcgat gcactacgat gggctaaaaa ggtgaaaacc ggcagttcat ccagtaagtc
     2821 agtattgacc tggcttgctg atatgtgcgg tgccgatttg tgtgcatacc cgtctgtatc
     2881 tgcactggca gaagtaacgg aactgaacaa aaagactgtg caggacagct tacgacacct
     2941 gatggagatt gggttaattg ttgataccgg tgagagaaaa ggcagaacaa agcaaattgt
     3001 ggtgtaccga cttatcggtg tagaagaaag tgttgccgag cctgaataca cccaaaaacg
     3061 ggagtcttta aaggtgggta aaattggtgc tgttaataaa aacagtaccg aaaatggtta
     3121 tgtttcagca caaaacagac ccaaaaacag aactcttagc tgcatggaaa ataaccaaag
     3181 acacccaaat tttccatcaa agacacccaa aaacggatca cggaacccaa aggaacccaa
     3241 agatctaaac cccacacata acgcacgcga gagtgctccg accagtgagc aggaagtttt
     3301 gtcgttacag gcagcccccc ctgtattcct gtatggcctg agcgaaccca tcggaaaatt
     3361 cccgatgagc gatagctggt atccgtcacg ggattttcga cgacgggctg cgttgtgggg
     3421 gatggctttg ccggagacag aatttacacc tgctgaactt gccgccttcc gggactactg
     3481 ggcagcggag gggaaagtgt ttacgcagat tcagtgggag cagaaattcg cccgtcacgt
     3541 aaatcacgtc agggcgcagg ttaaaccagt cagcaaaggg gtaaaccatg cagcagcacc
     3601 aggtggcacc gcatcacggg cagttcagga aattcgggca gcacgtgagc agtgggaacg
     3661 tgaaaacgga tttatcagcg acggaaacgg cctggaagct gtgggaactc atgggggtgg
     3721 tttattcgaa ccgctggatc cagaagaacg gggccgcacc ttcgaagctc tggattgcac
     3781 agattggcgc gatgactgag cagcaaatcc ggcaggtctg ccgccagtgc atggaccgct
     3841 gccgggcggg tgaaacatgg cctccggacc tggctgagtt tgtggcactg atttcgaaaa
     3901 gcggagccaa tccattcggt ctgacggtgg atgctgtgat ggaggagtac cgccgctggc
     3961 gcaatgagtc ctggcgatac gacggaagtg ataagtaccc gtggtctcag cctgtgctgt
     4021 atcacatttg cctcgagatg cgttcaaagg ggattgagcg ccagatgacc gaaggggaat
     4081 taaaacggct tgcagaacgg caactgacga aatgggcaaa gcatgttagt aacggcctga
     4141 gcgttccgcc agtccggcga caactggcgg cccccaaacg cccgtcgggg ccaacgccaa
     4201 ttgagttgct gaaacaggaa tatgaacgcc ggaaagcggc tggttttgtt tgagttgaga
     4261 agtgattttt taccgggagg aaatttatgg agactgtttt tgacgcactg aaagcgatgg
     4321 gaaaagccac gtcggtagag ctggctgcgc gacttgatat cagtcgtgaa gaagtactga
     4381 acgagctgtg ggaactgaaa aaggctggct tcgttgataa aagcgtatac acctggcgtg
     4441 tggctgataa caacgttcag caggaacagc cagcgccaga agaaaccacc acggcaacag
     4501 aagcgaaaat ctcagagtgc gatttaaccg cgacgattga acaacgcgga ccacaaacgg
     4561 cggatgaact ggctacgctg ttcggtacaa catcccgcaa agttgcttca acgctggcaa
     4621 tggcaatcag caaaggtcgt ctgattcgcg taaaccagaa cggtaaaatt cgttactgca
     4681 taccaggtga taatttacca gcagagccga aagttgaatc ggtagcggaa accgatggta
     4741 aagcctttcc tcagccagcc ggtgttcgtt accggtacag aaagatgcaa cacaggaaga
     4801 tattaaaaca gaaactgtgg cggacattgt gcagtcgctg ccatcgttta ctgcaacgcg
     4861 agaagatgat ttgattttgc catcgctgca tatggcaaat cgcgaactgc gtagggcgaa
     4921 gaatcatgtc cagaagtggg agcgagtctg cgccgcgctg cgggagctga acaagcaccg
     4981 ggatatggtt gccgggattt gtcggaagtc cgggcaatga gcggatggtg caggcctgaa
     5041 atcatgatac taacaatgaa ggtaaaatgc atcggcagtc tgattggtcg tagtgaggcg
     5101 gcggtcagga tgaaagccca ggttaaggga ataagcctga ttctgcgggg tgattttcac
     5161 cagtcaacaa aatatccgta gcgcgataac ggtcaaaaat tatggcgctg acacttttgt
     5221 gccactggag atgactgtac ctaagttcag gggagaagaa cacgtccggt gggatggtcg
     5281 ggccagattt aaagggcagg tcatggctcc agcctgtacg ctggcaatgg aggctgcctg
     5341 gcgggaaatt gatatgggaa ccacgccact cagggattta ctgccggtcc agagaataaa
     5401 ttcctgttac ggttacacca ctgtgatctt gcaagtgcag gaaagtaggt ctacacggta
     5461 acgcgagtgc gtgtaacttt tgatgtcatt cccgtagaaa caccggacaa attttcgctg
     5521 acaggtcatg cagaaggtat aaatctgcag attatggaca attacggata tccggcaaga
     5581 gccggaaaaa gcatgccgcc tctaattctc agtggaagat ggacttgatt atactcattg
     5641 cattgtcaga aatagttatc cattaaaggc tggctattcc aaacaggatg ttgattacaa
     5701 aaatgtaatc aacatgtaag gtttatactc ttcaatatgc gtataatttt ccttattttg
     5761 ttgactttaa ataacaagct atgcacgagg taaagtcgga taagtttatc tggatgtaat
     5821 atatattatt tgtagtgttt ataactttat ttcatgataa ccaataaaag gagtttttta
     5881 tgaggaacat aatggcaggt tttttaatat tcctgtcttc tgctgcttat gctgatatca
     5941 atctgtatgg tcctggtggc ccgcatacag ccttgcttga tgcagccaaa ctttatgccg
     6001 aaaaaacagg tattatagtg aacgttcatt acggcccaca gaacaaatgg aatgaagatg
     6061 ccaaaaaaaa tgcagatatc ttgtttggcg catcagaaca atctgctctg gctatcattc
     6121 gggaccataa agacagcttc agtgaaaaag atattcagcc tctttatctg cgaaaaagta
     6181 ttttactggt aaagaaaggt aatcctaaaa atatccggag tattgacgac ctgaccagac
     6241 ctgggattgg cgtaattgtt aatgatggtg gtggtaccag taatacatca ggcactggcg
     6301 tctgggaaga tattgccgga cgtaaaggga atatagaaac tgtcgccgca atccgaaaaa
     6361 atattatttt atatgcgccc aatagcggaa ctgcacgtaa ggctcttgag aatcagcctg
     6421 gagcagatgt ctggataacc tgggctgact gggcagccag taatccagaa attggtgatg
     6481 tcgtggaaat agcgccagac tacgtgatat ggcgtgatat gaacattaca gtacgtcagg
     6541 atgcaaatga tgaaacccgt cgatttgcag aatggctaca aaccgatgaa gcggcgcctg
     6601 cattcaaaaa atatggctgg accaggaaag gcacttgaca tcctcgtcct tcaggacgtg
     6661 gattcttttt ccggatgccg cgccagcggc atgtaggggc agctcacaaa acggaaaaaa
     6721 ttgtacgcta agcctcgcca ggtgaactga attcattccg atatgggaat tcccatatcg
     6781 ggcgaaaacg gtttgctgta acggcagagt taagtaggat tgctgcgggt gcttgaggct
     6841 atctgcctcg ggcatgaaca ccaacggcag atagataaaa gccccacccg actataaatc
     6901 gaagtgaggc ccctatatgc tcgtcacata tagattgcct cttacggacc gaaaggtcaa
     6961 ggagaagcag gctatgaagc agcaaaaggc gatgttaatc gccctgatcg tcatctgttt
     7021 aaccgtcata gtgacggcac tggtaacgag gaaagacctc tgcgaggtac gaatccgaac
     7081 cggccagacg gaggtcgctg tcttcacagc ttacgaacct gaggagtaag agaccaggcg
     7141 ggggagaaat ccctcgccac ctctgatgtg tcaggcatcc tcaacgcacc cgcacttaac
     7201 ccgcttcggc gggtttttgt ttttattttc aacgcgtttg aagttccgga cggcgccgga
     7261 atagaatcaa aaatacttaa gtagcgcgca gggagaagag ggatggaccc cgaacagggg
     7321 agtgctattt atctggaagg attctgttga tgagaatcga agaattacgt gaaattttta
     7381 gtgaagatgg cctctatact gtgcgcgttg agaagggcgc tattgtcagc cactgccgta
     7441 ttaaatgttt acagtctcaa caaaggaaga gtggagctgc gttaattcat tttgtggatg
     7501 ggcttgtgac ggatggtttt attttgcgtg caaatgaatt tgtcacatcg ttgccgtctc
     7561 tgaaagaagc tgggattaag gctggttttt ctgcttttga agattagtga attcatctac
     7621 aattcagcgc agggctgaac ccctgttgag taacactgtg ccaccggaga aagccgatgg
     7681 cgcaaaattc cagactacac aattctgata attcagccgt ctttgccagc aggcacgggc
     7741 ggcgttctca tgcattcaaa tctgactggt tccggcacgc cccatgcact gaagaacagg
     7801 ccgaatggct gattcagaac taccgcagac gtgggtatga gtttaggaaa gccctcagcc
     7861 tcgattatcg tcactggata atctacgtca ggctgccgta ctccgagcgc ccaccgcgtc
     7921 cgtcccgcac attccagcaa cgcatctgga ggtaacgtgc gggtattact tcgacctgtt
     7981 ccggtaccgg aacttgggtt ggtggtcctt aaaccaggcc gtgaatccat gcaggtattc
     8041 cataaccctc gagtgcttgt ggagccggaa ccgaaaagca tgcgcggtct gccgtccggc
     8101 atcgtccctg ccgttcgcca gccgctggcg gaggataaat cattactgcc gtttttcagc
     8161 aatgagcgtg tgattcgtgc tgttggtggc gctggtgcac tgtctgactg gctgttgcgt
     8221 catgtcaaat cctgccagtg gcctcatggt gactatcatc acagtgaaac cgttattcac
     8281 cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg acaaccagct gcgtgaccag
     8341 acatccgaat cactcgagca acttgctcat caaaacctgt cagcatggat gattgacgtc
     8401 atcggtcacg caataagcgg tacgcaggag cgtgaattat ctctggctga attatcctgg
     8461 tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag cggtattacg tcgttcgctg
     8521 gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa gcgacatcgt accgggagag
     8581 cagaccgcca ccatcatact gaagcagcgc acaaaaaatc ttgcgccgct gcctcacgcc
     8641 caccagcaaa acccgccaca ggaaaagacg gtggtcagca ttgccgttga tccggagtca
     8701 ccggctcagt atctccagcg ccagaaacca caacgggaag agatgcctgt atacacgcgc
     8761 tgggtaaaaa cgcagaaatg catgacgtg
//
LOCUS       DAZFKM010000177         8757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFKM010000177 DAZFKM010000000
VERSION     DAZFKM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(334..597)
                     /gene="rpsT"
                     /locus_tag="ACHKAY_004583"
     CDS             complement(334..597)
                     /gene="rpsT"
                     /locus_tag="ACHKAY_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO5703127.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            700..918
                     /locus_tag="ACHKAY_004584"
     CDS             700..918
                     /locus_tag="ACHKAY_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO5703128.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            926..1867
                     /gene="ribF"
                     /locus_tag="ACHKAY_004585"
     CDS             926..1867
                     /gene="ribF"
                     /locus_tag="ACHKAY_004585"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO5703129.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1910..4726
                     /gene="ileS"
                     /locus_tag="ACHKAY_004586"
     CDS             1910..4726
                     /gene="ileS"
                     /locus_tag="ACHKAY_004586"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO5703130.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4726..5220
                     /gene="lspA"
                     /locus_tag="ACHKAY_004587"
     CDS             4726..5220
                     /gene="lspA"
                     /locus_tag="ACHKAY_004587"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO5703131.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5308..5757
                     /gene="fkpB"
                     /locus_tag="ACHKAY_004588"
     CDS             5308..5757
                     /gene="fkpB"
                     /locus_tag="ACHKAY_004588"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO5703132.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5759..6709
                     /gene="ispH"
                     /locus_tag="ACHKAY_004589"
                     /gene_synonym="lytB"
     CDS             5759..6709
                     /gene="ispH"
                     /locus_tag="ACHKAY_004589"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO5703133.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6775..7689
                     /gene="rihC"
                     /locus_tag="ACHKAY_004590"
     CDS             6775..7689
                     /gene="rihC"
                     /locus_tag="ACHKAY_004590"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO5703134.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7856..8677
                     /gene="dapB"
                     /locus_tag="ACHKAY_004591"
     CDS             7856..8677
                     /gene="dapB"
                     /locus_tag="ACHKAY_004591"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO5703135.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1999 a         2163 c         2487 g         2108 t
ORIGIN      
        1 aggagatatt tatgaatggt taaagtgtat aggaatttaa ttcaaaaata gagtggtctt
       61 aagtttaata cctcaatgtg tatcacaata tccacattct ttgtagggga gtctggagat
      121 tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct taagccacga
      181 agagttcaga aagtgtaacg gcatgtctgc aaaagccagc atcttttgac tatctggcaa
      241 ccggtagtgt gttctctcac gcatcataaa agcagcaggc ataaaaaaac ccgcttgcgc
      301 gggctttttc acaaagcttc agcaaattgg cgattaagcc agtttgttga tctgtgcagt
      361 caggttagcc ttatgacgtg cagctttgtt tttgtggatc agacctttag cagcctgacg
      421 gtccacgatc ggttgcattt cgttaaatgc tttctgtgca gcagctttgt cgccagcttc
      481 gatagctgcg tatactttct tgatgaaagt acgcatcata gagcgacggc ttgcgttgtg
      541 cttacgagcc ttttcagact gaatggcgcg cttcttagct gatttgatat tagccaaggt
      601 ccaactccca aatgtgttct atatggacaa ttcaaaggcc gaggaatatg cccttttagc
      661 cttcttttgt caatggattt gtgcaaataa gcgccgttaa tgtgccggca ctcgttacgt
      721 agtgatggcg caggattcta ccagcttgca gggtgtgaat acagcttttc cgcgataaaa
      781 attgcagcag gcggtcactt tcttcccgtg atttgcgcca tggcaatgaa aagccacttc
      841 tttctgattt cggtactcaa tcgccggtta accttgaccg ctgtacaagg tatactcgga
      901 cgattttcac tgttttgagc cagacatgaa gctgatacgc ggcatacata atctcagcca
      961 ggccccgcaa gaagggtgtg tgctgactat tggtaatttc gacggcgtgc atcgcggtca
     1021 tcgcgcgctg ttacagggct tgcaggaaga agggcgcaag cgcaacttac cggtgatggt
     1081 gatgcttttt gaacctcaac cactggaact gtttgctacc gataaagccc cggcacggct
     1141 gacccggctg cgggaaaaac tgcgttacct tgcagagtgt ggcgttgatt acgtgctgtg
     1201 cgtgcgtttc gacaggcgtt tcgcggcgtt aaccgcgcaa aatttcatca gcgatcttct
     1261 ggtgaagcat ttgcgcgtaa aatttcttgc cgtaggtgat gatttccgct ttggcgctgg
     1321 tcgtgaaggc gatttcttgt tattacagaa agctggcatg gaatacggct tcgatatcac
     1381 cagtacgcaa actttttgcg aaggtggcgt gcgtatcagc agcaccgctg tgcgtcaggc
     1441 gctggcggat gacaatctgg ctctggcaga aagtttactg gggcacccgt ttgccatctc
     1501 cgggcgtgta gtccacggtg atgaattagg gcgcactata ggtttcccga cggcgaatgt
     1561 accgctacgc cgtcaggttt ccccggtgaa aggggtttat gcggtagaag tgctgggcct
     1621 cggtgaaaag ccgttacccg gcgtggcaaa cattggaaca cgcccaacgg ttgccggtat
     1681 tcgccagcag ctggaagtgc atttgttagg tgttgcaatg gacctttacg gtcgccatat
     1741 acaagtagtg ctgcgtaaaa aaatacgcaa tgagcagcga tttgcgtcgc tggacgaact
     1801 gaaagcgcag attgcgcgtg atgaattaac cgcccgcgaa ttttttgggc taacaaaacc
     1861 ggcttaagcc tgttatgtaa tcaaaccgaa acacggaacc gagaatctga tgagtgacta
     1921 taaatcaacc ctgaatttgc cggaaacagg gttcccgatg cgtggcgatc tcgccaagcg
     1981 cgaaccggga atgctggcgc gttggactga tgatgatctg tacggcatca tccgtgcggc
     2041 taaaaaaggc aaaaaaacct tcattctgca tgatggccct ccttatgcga atggcagcat
     2101 tcatattggt cactcggtta acaagattct gaaagacatt atcgtgaagt ccaaagggct
     2161 ttccggttat gactcgccgt atgtgcctgg ctgggactgc cacggtctgc cgatcgagct
     2221 gaaagtagag caagaatacg gtaagccggg tgagaaattc accgccgccg agttccgcgc
     2281 caagtgccgc gaatacgcgg cgacccaggt tgacggtcaa cgcaaagact ttatccgtct
     2341 gggcgtgctg ggcgactggt cgcacccgta cctgaccatg gacttcaaaa ctgaagccaa
     2401 catcatccgc gcgctgggca aaatcatcgg caacggtcac ctgcacaaag gcgcgaagcc
     2461 agttcactgg tgcgttgact gccgttctgc gctggcggaa gcggaagttg agtattacga
     2521 caaaacttct ccgtccatcg acgttgcttt tcaggcggtc gatcaggatg cactgaaagc
     2581 aaaatttgcc gtaagcaacg ttaacggccc aatctcgctg gtgatctgga ccactacgcc
     2641 gtggactctg cctgcgaacc gcgcaatctc tattgcacct gatttcgact atgcgctggt
     2701 gcagatcgac ggtcaggccg tgattctggc gaaagatctg gttgaaagcg taatgcagcg
     2761 tatcggcgtg accgattaca ccattctcgg cacggtaaaa ggtgcggagc ttgagttgct
     2821 gcgctttacc catccgttta tgggcttcga cgttccggca atcctcggcg atcacgttac
     2881 cctggatgcc ggtaccggtg ccgttcacac cgcgcctggc cacggcccgg acgactatgt
     2941 gatcggtcag aaatacggcc tggaaaccgc taacccggtt ggcccggacg gcacttatct
     3001 gccgggcact tatccgacgc tggatggcgt gaacgtcttc aaagcgaacg acatcgtcgt
     3061 tgcgctgctg caggaaaaag gcgcgctgct gcacgttgag aaaatgcagc acagctatcc
     3121 gtgctgctgg cgtcacaaaa cgccgatcat cttccgcgcg acgccgcagt ggttcgtcag
     3181 catggatcag aaaggtctgc gtgcgcagtc actgaaagag atcaaaggcg tgcagtggat
     3241 cccggactgg ggccaggcgc gtatcgagtc gatggttgct aaccgtcctg actggtgtat
     3301 ctcccgtcag cgcacctggg gcgtaccgat gtcactgttc gtgcacaaag acacggaaga
     3361 gctgcatccg cgtactctcg aactaatgga agaagtggca aaacgcgttg aagttgacgg
     3421 catccaggcg tggtgggatc ttgatgcgaa agagatcctc ggcgatgaag ctgatcagta
     3481 cgtgaaagtg ccggacacat tggatgtatg gtttgactcc ggatctaccc actcttctgt
     3541 tgttgacgtg cgtccggaat ttgccggtca cgcagcggac atgtatctgg aaggttctga
     3601 ccagcaccgt ggttggttca tgtcttccct aatgatctcc accgcgatga aaggcaaagc
     3661 gccgtatcgt caggtactga cccacggctt taccgtggat ggtcagggtc gcaagatgtc
     3721 taaatccatc ggcaataccg tttcgccgca ggatgtgatg aacaaactgg gcgcggatat
     3781 tctgcgtctg tgggtggcat caaccgacta caccggtgaa atggccgttt ctgacgagat
     3841 cctgaaacgt gctgccgata gctatcgtcg tatccgtaac accgcgcgct tcctgctggc
     3901 aaacctgaac ggttttgatc cagcaaaaga tatggtgaaa ccggaagaga tggtggtact
     3961 ggatcgctgg gccgtaggtt gtgcgaaagc ggcacaggaa gacatcctca aggcgtacga
     4021 agcatacgat ttccacgaag tggtacagcg tctgatgcgc ttctgctccg ttgagatggg
     4081 ttccttctac ctcgacatca tcaaagaccg tcagtacacc gccaaagcgg acagcgtggc
     4141 gcgtcgtagc tgccagactg cgctgtatca catcgctgaa gcgctggtgc gctggatggc
     4201 accaatcctc tccttcaccg ctgatgaagt gtggggctat ctgccgggcg aacgtgaaaa
     4261 atacgtcttt accggtgagt ggtacgaagg cctgtttggt ctggcagaca gtgaagcgat
     4321 gaacgatgcg ttctgggacg agctgttgaa agtgcgtggc gaagtgaaca aagtcattga
     4381 gcaagcgcgt gccgataaga aagtgggtgg ctcgctggaa gcggcagtaa ccttgtatgc
     4441 agaaccggaa ctggcggcga aactgaccgc gctgggcgat gaattacgat ttgtcctgtt
     4501 gacctccggc gctaccgttg cagactataa tgacgcacct gctgatgctc agcagagcga
     4561 agtactcaaa gggctgaaag tcgcgttgag taaagccgaa ggtgagaagt gcccacgctg
     4621 ctggcactac acccaggatg tcggcaaggt ggcggaacac gcagaaatct gcggccgctg
     4681 tgtcagcaac gtcgccggtg acggtgaaaa acgtaagttt gcctgatgag tcaatcgatc
     4741 tgttcaacag ggctacgctg gctgtggctg gtggtagtcg tgctgattat cgatctgggc
     4801 agcaaatacc tgatcctcca gaactttgct ctgggggata cggtcccgct gttcccgtcg
     4861 cttaatctgc attatgcgcg taactatggc gcggcgttta gtttccttgc cgatagcggc
     4921 ggctggcagc gttggttctt tgccggtatt gcgattggta ttagcgtgat cctggcagtg
     4981 atgatgtatc gctcgaaggc cacgcagaag ctaaacaata tcgcttatgc gctgattatt
     5041 ggcggcgcgc tgggcaacct gttcgaccgc ctgtggcacg gcttcgttgt cgatatgatc
     5101 gacttctacg tcggcgactg gcacttcgcc accttcaacc ttgccgatac tgccatttgt
     5161 gtcggtgcgg cactgattgt gctggaaggt tttttgcctt ctaaagcgaa aaaacaataa
     5221 taaaccctgc cggataaggc ctacagattg ctgcgaaatc gtaggccgca tccggcaaaa
     5281 atcctcaaaa tataagagca aacctgcatg tctgaatctg tacagagcaa tagcgccgtc
     5341 ctggtgcact tcacgctgaa actcgatgat ggcaccaccg ctgagtctac ccgcaacaac
     5401 ggtaaaccgg cgctgttccg cctgggcgat gcttctcttt ctgaagggct ggagcaacac
     5461 ctgttggggc tgaaagtggg cgataaaacc accttctcgc tggagcccga tgcggcgttt
     5521 ggcgtgccgt caccggacct gattcagtac ttctcccgcc gtgaatttat ggatgcaggc
     5581 gagccagaaa ttggcgcaat tatgcttttt accgcaatgg atggcagtga gatgcctggc
     5641 gtgatccgcg aaattaacgg cgactccatt accgttgatt tcaaccatcc gctggccggg
     5701 cagaccgttc attttgatat tgaagtgctg gaaatcgatc cggcactgga ggcgtaacat
     5761 gcagatcctg ttggccaacc cgcgtggttt ttgtgccggg gtagaccgcg ctatcagcat
     5821 tgttgaaaac gcgctggcca tttacggcgc accgatatat gtccgtcacg aagtggtaca
     5881 taaccgctat gtggtcgata gcttgcgtga gcgtggggct atctttattg agcagattag
     5941 cgaagtaccg gacggcgcga tcctgatttt ctccgcacac ggtgtttctc aggcggtacg
     6001 taacgaagca aaaagtcgcg atttgacggt atttgacgcg acctgtccgc tggtgaccaa
     6061 agtgcatatg gaagtcgccc gcgccagtcg ccgtggcgaa gaatctattc tcatcggtca
     6121 cgccgggcac ccggaagtgg aagggacaat gggccagtac agtaacccgg aagggggaat
     6181 gtatctggtc gaatcgccgg acgatgtgtg gaaactgacg gtcaaaaacg aagagaagct
     6241 ctcctttatg acccagacca cgctgtcggt ggatgacacg tctgatgtga tcgacgcgct
     6301 gcgtaaacgc ttcccgaaaa ttgtcggtcc gcgcaaagat gacatctgct acgccacgac
     6361 taaccgtcag gaagcggtac gcgccctggc agaacaggcg gaagttgtgt tggtggtcgg
     6421 ttcgaaaaac tcctccaact ccaaccgtct ggcggagctg gcccagcgta tgggcaaacg
     6481 cgcgtttttg attgacgatg cgaaagatat ccaggaagag tgggtgaaag aggttaaatg
     6541 cgtcggcgtg actgcgggcg catcggctcc ggatattctg gtgcagaatg tggtggcacg
     6601 tttgcagcag ctgggcggtg gtgaagccat tccgctggaa ggccgtgaag aaaacattgt
     6661 tttcgaagtg ccgaaagagc tgcgtgtcga tattcgtgaa gtcgattaag tcattagcag
     6721 cctaagttat gcgaaaatgc cggtcttgtt accggcattt tttatggaga aaacatgcgt
     6781 ttacctatct tcctcgatac tgaccccggc attgacgatg ccgtcgccat tgccgccgcg
     6841 atttttgcac ccgaactcga cctgcaactg atgaccaccg tcgcgggtaa tgtctcggtt
     6901 gagaaaacta cccgcaatgc cctgcaactg ctgcatttct ggaatgcgga gattccgctc
     6961 gcccaagggg ccgctgtgcc gctggtacgc gcaccgcgtg atgcggcatc tgtgcacggc
     7021 gaatcgggaa tggctggcta cgactttgtt gagcacaacc gaaagccgct cgggataccg
     7081 gcgtttctgg cgattcggga tgccctgatg cgtgcaccag agcctgttac cctggtggcc
     7141 atcggcccgt taaccaatat tgcgctgtta ctttcacaat gcccggaatg caagccgtat
     7201 attcgccgtc tggtgatcat gggtggttct gccggacgcg gcaactgtac gccaaacgcc
     7261 gagtttaata ttgctgccga tccagaagct gctgcctgtg tcttccgcag tggtattgaa
     7321 atcgtcatgt gcggtttgga tgtcaccaat caggcaatat taactcctga ctatctctct
     7381 acactgccgc agttaaaccg taccgggaaa atgcttcacg ccctgtttag ccactaccgt
     7441 agcggcagta tgcaaagcgg cttgcgaatg cacgatctct gcgccatcgc ctggctggtg
     7501 cgcccggacc tgttcactct caaaccctgt tttgtggcag tggaaactca gggcgaattt
     7561 acctcaggca cgacggtggt tgatatcgac ggttgcctgg gcaagccagc caatgtacag
     7621 gtggcattgg atctggatgt gaaaggcttc cagcagtggg tggctgaggt gctggctctg
     7681 gcgtcgtaac ctgtcacatg ttattggcat gcagtcattc atcgactcat gcctttcact
     7741 gatatccctc cctgtttatc attaatttct aattatcagc gtttttggct ggcggcgtag
     7801 cgatgcgctg gttactctga aaacggtcta tgcaaattaa caaaagagaa tagctatgca
     7861 tgatgcaaac atccgcgttg ccatcgcggg agccgggggg cgtatgggcc gccagttgat
     7921 tcaggcggcg ctggcattag aaggcgtgca gctgggcgcg gcgctggagc gcgaaggatc
     7981 ttctttactg ggcagcgacg ccggtgagct ggctggtgca gggaaaacag gcgttaccgt
     8041 gcaaagcagc ctcgatgcgg taaaagatga ttttgatgtg tttatcgatt ttacccgtcc
     8101 ggaaggtacg ctgaaccatc tcgctttttg tcgccagcat ggcaaaggga tggtgatcgg
     8161 cactacgggg tttgacgaag ccggtaaaca ggcgattcgt gacgccgccg ccgatattgc
     8221 gattgtcttt gccgccaatt ttagcgttgg cgttaacgtc atgcttaagc tgctggagaa
     8281 agcagccaaa gtgatgggtg actacaccga tatcgaaatt attgaagcac atcatagaca
     8341 taaagttgat gcgccgtcag gcaccgcact ggcaatggga gaggcgatcg cccacgccct
     8401 tgataaagat ctgaaagatt gcgcggtcta cagtcgtgaa ggccacaccg gtgaacgtgt
     8461 gcctggcacc attggttttg ccaccgtgcg tgcaggtgac atcgttggcg aacataccgc
     8521 gatgtttgcc gatattggcg agcgtctgga gatcacccat aaggcgtcca gccgcatgac
     8581 atttgctaac ggcgcggtaa gatcggcttt gtggttgagt ggtaaggaaa gtggtctttt
     8641 tgatatgcga gatgtgcttg atctcaatag tttgtaacca caaaatattt gttatggtgc
     8701 aaaaataaca catttaattt attgattgta aagggcttta atttttggcc cttttat
//
LOCUS       DAZFKM010000178         8738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFKM010000178 DAZFKM010000000
VERSION     DAZFKM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..610
                     /locus_tag="ACHKAY_004592"
     CDS             <1..610
                     /locus_tag="ACHKAY_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO5703136.1"
                     /translation="HCPYIKDPIIRSYIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHF
                     INRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIE
                     DNLRTFNTNELTRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSR
                     HKMLPINLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
     gene            833..1330
                     /gene="ubiC"
                     /locus_tag="ACHKAY_004593"
     CDS             833..1330
                     /gene="ubiC"
                     /locus_tag="ACHKAY_004593"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO5703137.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1343..2215
                     /gene="ubiA"
                     /locus_tag="ACHKAY_004594"
     CDS             1343..2215
                     /gene="ubiA"
                     /locus_tag="ACHKAY_004594"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO5703138.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2370..4793)
                     /gene="plsB"
                     /locus_tag="ACHKAY_004595"
     CDS             complement(2370..4793)
                     /gene="plsB"
                     /locus_tag="ACHKAY_004595"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO5703139.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4964..5332
                     /gene="dgkA"
                     /locus_tag="ACHKAY_004596"
     CDS             4964..5332
                     /gene="dgkA"
                     /locus_tag="ACHKAY_004596"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5703140.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5442..6050
                     /gene="lexA"
                     /locus_tag="ACHKAY_004597"
     CDS             5442..6050
                     /gene="lexA"
                     /locus_tag="ACHKAY_004597"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO5703141.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6123..7448
                     /gene="dinF"
                     /locus_tag="ACHKAY_004598"
     CDS             6123..7448
                     /gene="dinF"
                     /locus_tag="ACHKAY_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO5703142.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7564..7773
                     /gene="yjbJ"
                     /locus_tag="ACHKAY_004599"
     CDS             7564..7773
                     /gene="yjbJ"
                     /locus_tag="ACHKAY_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO5703143.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7815..8330)
                     /gene="zur"
                     /locus_tag="ACHKAY_004600"
     CDS             complement(7815..8330)
                     /gene="zur"
                     /locus_tag="ACHKAY_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO5703144.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8648..>8738
                     /gene="yjbL"
                     /locus_tag="ACHKAY_004601"
     CDS             8648..>8738
                     /gene="yjbL"
                     /locus_tag="ACHKAY_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO5703145.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDK"
BASE COUNT         2053 a         2080 c         2340 g         2265 t
ORIGIN      
        1 ccattgtcct tatataaaag atccaattat acgcagttat atccattctg gtgaagataa
       61 caagttcgat gattttatgc gtcaacatcg attcagcaag gtggatttcg atacccaaca
      121 gatgatccat tttattaaca ggtttaatat gaataaaggg ctgattgata aaaataacaa
      181 tttttttatc caacttatcg atcaggcctt acgatcaacg gatgatatga tcaaagcaaa
      241 tgcctggtat ctttataaag agtggattcg tagtgatgat gtttcacctc tatttataga
      301 aattgaagat aatttaagaa cctttaacac gaatgaatta acacgaaagg ataatatctt
      361 tatcctgttc tcctctgccg atgatgggcc agttatggtg gtaagctccc agcgcttaca
      421 tgatatgttg aatcctacaa aagataccaa ttggaattcc acgtgtatct ataaatccag
      481 acataagatg ttgcctatta atcttactca ggaaacactt tttagctcca aatctcatgg
      541 taaatatgcg cttttcccaa tttttactgc gagttggcga gctactcgta taaagaatat
      601 aggtatttaa gtaaaggaaa acatcaccgt tcctggcatc atgggcggtg atgcccctac
      661 ggttgccctc gccagcacgg gcatcggcaa agcgtaaggt tcaacagcgg tttaccactt
      721 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      781 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      841 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      901 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      961 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
     1021 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
     1081 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
     1141 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
     1201 gacattaacc cgggacttta ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc
     1261 ccgcctgcga ttaagcggta aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc
     1321 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
     1381 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1441 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1501 cgcgggtgtc tggctgatgc gcgctgctgg atgtgtggtg aatgattatg ctgaccgaaa
     1561 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1621 gaaagaggcg cgcgcactgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1681 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1741 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1801 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1861 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1921 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1981 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     2041 tggcttaggc tggggatatt actggtcaat tgtggtggct ggcgcgctgt ttgtttatca
     2101 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     2161 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     2221 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     2281 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaaggcc ggataaggcg
     2341 ttttcgccgc atccggcaat tctctctgat tacccttcgc cctgcgtcgc actctcaatc
     2401 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2461 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagtgt cagcaccaga
     2521 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga tgccgtgcag cacggagaga
     2581 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttggca
     2641 ctcaacaacc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggcgagc
     2701 agctgtagcg tgcgagaatg caccgggttg atatgcaact catcatcttg cagggtaatc
     2761 agcccctgac gttgcatctc atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga
     2821 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2881 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2941 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgtggc
     3001 agaatgatga tgtcgccgat agtgtctttc tcgacttcaa acttgttcat ttgcagcgcg
     3061 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     3121 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtgagtgag
     3181 cgctgacgtg atgccagtag cgcagtacag cacaggttca tggcgtttgc cgcgcctgcg
     3241 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccaggcc
     3301 ggacgcaccg cttcgatggg atcgatagat tcacgccagt ctggtacgtg ctggttaagg
     3361 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3421 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc gccgcgcagt
     3481 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3541 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3601 ttcggatcca gcaaacgccc cgtgcgggaa cgaccgcctt ccacgaagta ctcgacggaa
     3661 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3721 cctttaaaag tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3781 cagaagttca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3841 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3901 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3961 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     4021 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     4081 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     4141 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     4201 aagtgcatac gcgccacgcg cgccagtttc tgagcgatag ttttatctgt gccgtgttca
     4261 tcggccatgc ggcgcagaga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     4321 cacagtacgg cgaaaaattt ttgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     4381 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4441 agatttgggt tgctgcggtg caaatcaaga tagtcgtgga acagcttaat agactcttct
     4501 ttcggcgtgt aataggtgaa cacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4561 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4621 gcgcgcaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4681 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4741 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4801 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4861 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4921 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4981 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     5041 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     5101 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     5161 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     5221 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgccat
     5281 tatcgtcgcc gtgattacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     5341 gatttcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     5401 atatactcac agcataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5461 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5521 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5581 tgaaggcgct ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc
     5641 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5701 cgcttctggc gcaacagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc
     5761 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg
     5821 atggtgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5881 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5941 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     6001 ccattgaagg gctggcggtt ggggttattc gcaacggcga ctggctgtaa catatctctg
     6061 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     6121 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     6181 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     6241 atagcccggt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     6301 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     6361 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggctg
     6421 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcgctgcat attgttggcg
     6481 gtagtgaagc ggtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgctc
     6541 cggcgtcgct ggcgaatctg gtattactcg gttggttact cggcgtgcaa tatgcccgtg
     6601 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6661 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6721 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6781 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6841 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgataacc gtacttggtg
     6901 cgcgactggg gagtgacatt gtcgctgtta acgcggttct gatgacgcta ctcaccttta
     6961 ccgcctatgc gcttgatggt tttgcctacg cggttgaagc gcactccggt caggcatacg
     7021 gtgcgcgcga cggtagccag ttgctggatg tatggcgggc agcgtgccgc cagtcgggga
     7081 ttgtagcgtt actgttttcg gtggtttatt tgctggcagg ggaacacatc attgcgttgc
     7141 tgacgtcgtt aacccagatt cagcagctgg ctgaccgcta tcttatctgg caggtgattt
     7201 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgtg
     7261 ccgccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     7321 cgctgccgtg gctgggtaat catggtttgt ggctggcatt aaccgtcttt ctggcgttgc
     7381 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7441 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7501 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7561 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7621 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7681 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7741 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7801 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7861 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7921 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat aatgtcttcc
     7981 acgccttctg cacactcttc tttcaccgcg ccgcagcgat cgcaaataaa catggctgac
     8041 gtatgggtgg gctggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     8101 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     8161 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     8221 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     8281 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     8341 aaagtggggg gtaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     8401 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttgtgtt gccttgtcgt
     8461 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8521 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8581 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8641 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8701 aaatcaattc gataatgaag atgcaatcaa agataaat
//
LOCUS       DAZFKM010000179         8731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFKM010000179 DAZFKM010000000
VERSION     DAZFKM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(624..2084)
                     /gene="phoQ"
                     /locus_tag="ACHKAY_004602"
     CDS             complement(624..2084)
                     /gene="phoQ"
                     /locus_tag="ACHKAY_004602"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO5703146.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYIVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2084..2755)
                     /gene="phoP"
                     /locus_tag="ACHKAY_004603"
     CDS             complement(2084..2755)
                     /gene="phoP"
                     /locus_tag="ACHKAY_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO5703147.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(2923..4293)
                     /gene="purB"
                     /locus_tag="ACHKAY_004604"
     CDS             complement(2923..4293)
                     /gene="purB"
                     /locus_tag="ACHKAY_004604"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO5703148.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4297..4938)
                     /gene="hflD"
                     /locus_tag="ACHKAY_004605"
     CDS             complement(4297..4938)
                     /gene="hflD"
                     /locus_tag="ACHKAY_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO5703149.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(4974..6080)
                     /gene="mnmA"
                     /locus_tag="ACHKAY_004606"
                     /gene_synonym="trmU"
     CDS             complement(4974..6080)
                     /gene="mnmA"
                     /locus_tag="ACHKAY_004606"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO5703150.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6134..6595)
                     /gene="nudJ"
                     /locus_tag="ACHKAY_004607"
     CDS             complement(6134..6595)
                     /gene="nudJ"
                     /locus_tag="ACHKAY_004607"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO5703151.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(6605..7258)
                     /gene="rluE"
                     /locus_tag="ACHKAY_004608"
     CDS             complement(6605..7258)
                     /gene="rluE"
                     /locus_tag="ACHKAY_004608"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO5703152.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7430..8680
                     /gene="icd"
                     /locus_tag="ACHKAY_004609"
     CDS             7430..8680
                     /gene="icd"
                     /locus_tag="ACHKAY_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO5703153.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         2138 a         2214 c         2212 g         2167 t
ORIGIN      
        1 cggcggtaac tcactgagat taaagcaaag cgcaaaattg cgcggtcggg cagaatgacc
       61 accacctcaa tcgtgacgga gagtgacaac cagtgacaca ccgtgacaaa aagacggttt
      121 aaccgttcat taaaaatcga cgtgattttg tgctttccgg atgacgtggc caacgtcatt
      181 ttttagcgaa aaattctgat taaggtcact ggctgaatgg tcataatgac cacgcagacc
      241 atcgaggcaa ccatagcgat catcgttacg gttggagctt ttccgccatt ggtggaaaag
      301 gtgcacatct ggatcatcat tacggtgatc aagggtatgt aaaacccacc agcctgatta
      361 aaggttaacc ggaaaaaaac aggtggcccc gttatgggga tccccatatc gacattaacg
      421 cccctcatga attggtgcgc ttccccctgg aaagattacc cgcctgtata tttcttgtgt
      481 ctatttgttc cacgttgttt catacagtgc accgaacggt gtagttaatg gtgtagtcaa
      541 tttgctgttt ttagcacttt ttgcagtgtg agttgtttaa ttaaatcaat tggttgcctt
      601 gctgtggtga taaaaggaca tatttattca tctttcggcg cagaatgctg gcgaccaaaa
      661 atcacctcca tccgcgcacc gcccagcatg ctctctccgg cgacgatttt accctcatat
      721 tgctcggtga tttcgcgggc taccgccagc cctacacctt gcccagggcg taaagtatca
      781 acccgttgac cacggtcgaa aatgacctct cgcttgctta atggaatacc ggggccatca
      841 tcctcgacca caatatagag atgctcgtcg gtttgccttg cagaaatttc gacaaactcg
      901 aggcaatatt tacaggcatt atccagcacg ttgcccatca cctcgacaaa atcgttctgc
      961 tcaccgacaa agctgatctc tggcgaaata tcgagagaga tattgacccc tttgcgttga
     1021 tacactttgt tcagcgctga ggtgagattg tccagcagtg gggcgaccgg atgcagctcg
     1081 cggctgagca atgtcccgcc gcgcatactg gcacgatgca ggtagtagcc aatttgctgt
     1141 gaaatgcggc tgatttgctc cagcattacc ggctcagcat cactgacgct catcttttca
     1201 ctacgcagag aacgcagcgt actttgcagc accgccagtg gcgttttcag actatgggtc
     1261 aggtcggtga gcgtcgtacg gtatttgtcg taacgttcgc gttcactttt taacaatcgg
     1321 ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg ctggattgag caattcgcgg
     1381 ttatgttctt ccagttcgcg gacttctttt gccagggctt cgatggggcg taaactccac
     1441 caggcggcga cccacagcag cgggatcact aacagcagat tggctgagag cacatagata
     1501 aaccagctcc agactatata ggaacttttt agctccaccg gaatggtatc caccaccaca
     1561 atggttaatt ttggcatccg cgatgttgcc gggtagacgt ttactgccac cgagtgggtc
     1621 atctccgcgt cgtcatcatc ttcccgcact tcctgcaact gttgctgtat cgaatgatct
     1681 ccactcagca agaggctggt atcgttaaca tccgcttcaa tttcatgaaa accattcgat
     1741 ttcagccagt caggctggat catcttcatc agccagggca cgtcacgttg cgcccataaa
     1801 agctgcccgt tctcatcata aattagcgtc atggtggggc tttgcttgtc gatattttcg
     1861 ggtaactcga catgcaactt attgttttcc cacttcgcaa gggtatagaa cagattgctc
     1921 tcgccacgta acagccgaaa cgtagtttta tcgaaactga cgctataacc gatcagcgcg
     1981 accattccgt aggcaagcga aagcaccagt actaccgcag ccgttgccaa cagaaaacgt
     2041 acccgcagtg agagcgggaa aaaaagacgc agtaattttt tcatcagcgc aattcgaaca
     2101 aatagccctg gccgcgaacg gtagtaatca cttcttgcgg atattgtgcc tgaatttttt
     2161 tgcgcagacg tcccatcagt acatcaatgg tatggctttc ccgcagctcc gcatccggat
     2221 agagttggag cattaacgaa tctttgctca ccactttgcc attattgcgt atcaacgttt
     2281 ccataatggt gtattcgaac gcggtcagtt tgatcacttc gtcattaata gataattcac
     2341 ggcgagagag atcaacctga aacgggggta gcgaaatgac ctgtgaagcc aggccgctat
     2401 tacgccgcat taatgcctgc attcgcgcca tcacctcttc aatatgaaac ggtttagtca
     2461 cgtaatcatc agcaccggca cttaatactt cgactttgtc ctgccagctt tcacgggcgg
     2521 ttaataccag aatcggcagt gaaacatcat tgctacgcca gcggcgaatc agtgacagac
     2581 cgtcctcgtc aggcaatccg agatcgacaa tcgcaatatc cggtaaatgt tcattgagat
     2641 aataatcggc ttctttggca tcttctgcgt catcgacctg atgaccagca tcctgaatct
     2701 gaactttaag gtggtgacgt aacaacgcat tgtcttcaac aaccagtacg cgcattttta
     2761 tttctccctg tcttaattat taaaatagtg taacgcgatt atgtgtttat gggggtaaac
     2821 attaaataaa ccagcgggga ggggaggtaa agtgaaaaaa gaaaaagtgg ataattttaa
     2881 taaacaggcc ggacagcatc gccatccggc actgatacga gtttatttca gctcatcaac
     2941 catggtgata gctcgaccaa tatagttagc cggcgtcatc gctttcaggc gggctttctc
     3001 ttcttctggc aacgccagac catcgataaa ctgcttcatg ccttcggcgt caacgcgctt
     3061 accgcgagtc agctctttca gcttctcgta cggtttttcg atgccatagc gacgcataac
     3121 tgtctggatt ggctcagcca gcacttccca gttgtgatcc agctcatcca gcagatggtc
     3181 acggtttact tccagtttgc tcacgccttt cagggtggat tgatacgcaa tcagcgcata
     3241 accgataccc acgccgaggt tacgcagcac ggtggagtcg gtcaggtcac gctgccagcg
     3301 ggaaaccggc agtttgcttg ccagatgctg caataccgcg ttggaaaggc ccagattccc
     3361 ttcggagttt tcgaagtcaa tcgggttaac tttatgcggc atggtggaag aaccaatctc
     3421 accagcaatg gttttctgtt tgaagtggtt aagggcgata taaccccaga cgtcacggtc
     3481 aaagtcgatc agaatggtgt tgaagcgcgc aacgcaatca aacaattcgg caatgtagtc
     3541 gtgcggttcg atctgggtgg tgtacgggtt ccactgaata cccagcgagg tgacgaactc
     3601 ttcgctgaac tgatgccagt caacttccgg gtaagcggcg atatgggcgt tatagttacc
     3661 gaccgcgccg ttgattttgc cgaggatctc cacctgatta agctggcggt actggcgctc
     3721 catacggtag gcgacgtttg ccatctcttt accgatggtt gacggcgtgg ctggctgacc
     3781 gtgggtacga gacagcagcg ggatatcgcg gtactgaacg gcgagatctt taatgccatc
     3841 aatcagttga cgccagtacg gcaggatcac ttcatcacgc gcggttttca gcattaatgc
     3901 gtgggagagg ttattgatat cttccgaagt acaggcaaag tggatgaatt cagaaaccgc
     3961 gtgcagttcc gggatctccg ccactttttc tttcaggaaa tactcaaccg ctttaacgtc
     4021 gtggttagtg gtacgctcga tggttttgat gcgtgcggca tcttcttcgc tgaaattggc
     4081 gacaattgca tcaaggtaac cgattgcgtc ggcagcaaaa gcaggaactt ccttgatcgc
     4141 tgcgtgcgcg gccagttttt gcagccaacg tacttcaact tgtacacgga atttcagcaa
     4201 accatattcg ctgaaaattc cgcgcagcgc gctgacttta tcgccgtagc gtccatcgac
     4261 aggggaaacg gcggtcagtg aggataattc catagatcac aactccgggg ttaaatgagc
     4321 aagaatttgt tttgcctgag tggtcaggcg attacgagaa aacatcagtt gcagacgtcc
     4381 gccgccgacc tggtgccaga gcacggcggc gcgaatgcct gccagcaggg ttgcgcgaac
     4441 tttcgcctgc acttgtgggc tttgcagtac agcaggggaa ccggtgacct gaatgcgcgg
     4501 gccaagcggg ctgatcacat caacatagat agcagccatc gcgctcatca gcgtttcgga
     4561 ctgtaaatcg aagtgttcga gctggcgttg caggccgttg atccggttgc ccagagtgtc
     4621 gagcgcgcct ttcgctgagg agagtttgcg ctcaagcacc atcaagctga gtgtgtagcg
     4681 ggttaattcg gcgtttaagc cctggcgact gctggcattg agcacgccga gcagggtttc
     4741 cagcccgacg cgcaggttgg cttcgctacc gccaaaaacc gccagcgtcg agctggggtt
     4801 catatcaata atactgttga gtgagacgtg tagcgcatcg gcatcacaat gcccctggtg
     4861 agcgagttgt tgcaccaggc gtgccgactg acaaataccg gccagggcga gggtgatgtc
     4921 atagtaattc tttgccacgt tcactgcttc cttgttttaa gtaaagataa taatcagacc
     4981 ggcagcggca gacgctgctc aataataccg ccaccgaggc acacttcacc gttatagaag
     5041 acggcagact ggcccggcgt cacggcggca accggttcat cgaaaatcac ttcaatgcga
     5101 tcatcgtcca gcgccttgac ggtgcaaggg atatcggtct ggcgatagcg ggtttttacc
     5161 gtgcaacgca tagtgccggt gaatggttcg cgatcgaccc agtgcaactg ctgggcaatc
     5221 aacccgacag acatcagccg cgggtgttca tggccctgag cgacaaccag aatgttgttt
     5281 tcgacgtctt tgtccaccac ataccacggt tcttcggtac cttctttggt gccaccgata
     5341 cccagacctt tacgctgacc gagagtgtga tacatcagcc cctggtgctc accaatttca
     5401 tcgccatcga cggtaatgat tttgcccggt tgcgccggga gataacggcc caggaactcg
     5461 cggaatttac gttcgccaat gaagcaaatg ccggtagagt cttttttctt cgcggtgacc
     5521 agaccaagat cttcagcaat cttacgcacc tgcggttttt ccagttcgcc gaccgggaac
     5581 aggctttgcg caatctgctc atggctgagc gtataaagga agtagctctg gtctttattg
     5641 ctgtccagac cacgcagcag gcggctcttg ccatcgacat cggcccgacg tacgtaatga
     5701 ccggtagcga tataatcggc acctaaatct tcggcggcaa attcgaggaa ggctttaaat
     5761 ttgatctctt tgttgcacag aatatccgga ttcggcgtgc gaccggcttt atattcggca
     5821 aggaacagtt cgaagacgtt gtcccagtac tcggcagcaa agttaacggt gtgcagttca
     5881 atgccgagct tgtcgcagac agcctgggca tcagccagat ccgctgccgc tgtgcaatat
     5941 tcctcaccgt cgtcttcttc ccagttcttc ataaacaggc cttcgacctg atatccctgt
     6001 tgttgcaaca gccaggcaga aacggaggaa tcgacaccgc cggacatgcc gacgattact
     6061 ttttttgcgg tttcagacat tggatcactc acgacattga acttcaaggc ggcgtattct
     6121 atcacgcagc cttttagatg acaccctttg taaaaggcca gttaaaatcg ccaatcatct
     6181 ccagcggata gcgttgcccg ctttgataac aacggatact ttccgccacc agcggcgaac
     6241 gaagatttga cgcctgtaaa atttcttcgg cgctgaccca acgacagcag tcgatatcgc
     6301 tgtcatgagg ctgggtcggg catatttgct caagctcaat ggcaaagagg aaacgcaaaa
     6361 acggcgtttt atctggcgca atccactgat gcatacgaat aaagtgttgc ggctgcgcgc
     6421 tgatgccggt ttcttcccac agttcacggg cggcggcttc cactaaggtt tcatcggctt
     6481 ccagatgccc ggcaggttgg ttccataacg ctttaccatt aatcgtctct tcaacgacta
     6541 aaaatttgcc ttctgcgtgc accacgcaag caacggtaac gtgcggttta aacattttgc
     6601 atccttaatc tgtcacttct cgccattcgc cattggcaag attatccaaa gagtaatctc
     6661 ccatcgcata gcgaatcagt cgcagcgtgg ggaaaccaac atgggcggtc atgcggcgca
     6721 cctggcgatt acgtccttca tataaggtga tcttcagcca gctggtggga atacttttgc
     6781 gttcacgaat tggtggattc cgcggccata accacgcggg ttcgtcaacc agctccgcgc
     6841 ctgcgggcag ggtagggcca tcatttaagg ttacgccatt gcgcaaggct tcaagtgcgt
     6901 cttgtgtggg aataccttcc acctgcacat aatagatttt cccggtgcgt ttgcccggct
     6961 gggttaaacg cgcctgcaac gcgccgttat tggtcagcac cagtaaccct tcgctatcgc
     7021 ggtcaagacg acctgctgca taaacgccct gaaccgggat gaattctttt aatgttttgc
     7081 gtccggcttc atcggtgaac tgcggaagaa catcgtaggg tttattgaac aggatcacac
     7141 gcgtgggctg gttttcaggt ttacgcctgg tagaacgttg cgagctgaat cgcttaactt
     7201 ggtgatttct aaaagaagtt ttttgcatgg tattttcaga gattatgaat tgccgcatta
     7261 tagcctaata acgcgcatct ttcatgacgg caaacaatag ggtagtattg acaagccaat
     7321 tacaaatcat taacaaaaaa ttgctctaaa gcatccgtat cgcaggacgc aaacgcatat
     7381 gcaacgtggt ggcagacgag caaaccagta gcgctcgaag gagaggtgaa tggaaagtaa
     7441 agtagttgtt ccggcacaag gcaagaagat caccctgcaa aacggcaaac tcaacgttcc
     7501 tgaaaatccg attatccctt acattgaagg tgatggaatc ggtgtagatg taaccccagc
     7561 catgctgaaa gtggtcgacg ctgcagtcga gaaagcctat aaaggcgagc gtaaaatctc
     7621 ctggatggaa atttacaccg gtgaaaaatc cacacaggtt tatggtcagg acgtctggct
     7681 gcctgctgaa actcttgatc tgattcgtga atatcgcgtt gccattaaag gtccgctgac
     7741 cactcctgtt ggtggcggta ttcgctctct gaacgttgcc ctgcgccagg aactggatct
     7801 ctacatctgc ctgcgtccgg tacgttacta tcaaggcact ccaagcccgg ttaaacaccc
     7861 tgaactgacc gatatggtta tcttccgtga aaactcggaa gacatttatg cgggtatcga
     7921 atggaaagca gactctgccg acgccgagaa agtgattaaa ttcctgcgtg aagagatggg
     7981 ggtgaagaaa attcgcttcc cggaacattg tggtatcggt attaagccgt gttcggaaga
     8041 aggcaccaaa cgtctggttc gtgcagcgat cgaatacgca attgctaacg atcgtgactc
     8101 tgtgactctg gtgcacaaag gcaacatcat gaagttcacc gaaggcgcgt ttaaagactg
     8161 gggctaccag ctggcgcgtg aagagtttgg cggtgaactg atcgacggcg gcccgtggct
     8221 gaaagttaaa aacccgaaca ccggcaaaga gatcgtcatt aaagacgtga ttgctgatgc
     8281 attcctgcaa cagatcctgc tgcgtccggc tgaatatgat gttatcgcct gtatgaacct
     8341 gaacggtgac tacatttctg acgctctggc agcgcaggtt ggcggtatcg gtatcgcccc
     8401 tggagcaaac atcggtgacg aatgcgccct gtttgaagcc acccacggta ctgcgccgaa
     8461 atacgccggt caggacaaag taaaccctgg ctctattatt ctctccgctg agatgatgtt
     8521 acgccatatg ggctggactg aagccgcaga cctgattgtt aaaggtatgg aaggcgcaat
     8581 caatgcgaag accgtaacct atgacttcga gcgtctgatg gaaggcgcta aactgctgaa
     8641 atgttcagaa tttggtgatg cgatcatcaa gaacatgtaa tcactacatg tgtttaatat
     8701 tgcaacgggc gtataacacg cccgttgttt t
//
LOCUS       DAZFKM010000180         8706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFKM010000180 DAZFKM010000000
VERSION     DAZFKM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..1547
                     /gene="ulaA"
                     /locus_tag="ACHKAY_004610"
     CDS             150..1547
                     /gene="ulaA"
                     /locus_tag="ACHKAY_004610"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO5703154.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1563..1868
                     /gene="ulaB"
                     /locus_tag="ACHKAY_004611"
     CDS             1563..1868
                     /gene="ulaB"
                     /locus_tag="ACHKAY_004611"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO5703155.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1878..2342
                     /gene="ulaC"
                     /locus_tag="ACHKAY_004612"
     CDS             1878..2342
                     /gene="ulaC"
                     /locus_tag="ACHKAY_004612"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO5703156.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2356..3006
                     /gene="ulaD"
                     /locus_tag="ACHKAY_004613"
     CDS             2356..3006
                     /gene="ulaD"
                     /locus_tag="ACHKAY_004613"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO5703157.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3016..3870
                     /gene="ulaE"
                     /locus_tag="ACHKAY_004614"
     CDS             3016..3870
                     /gene="ulaE"
                     /locus_tag="ACHKAY_004614"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO5703158.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3870..4556
                     /gene="ulaF"
                     /locus_tag="ACHKAY_004615"
     CDS             3870..4556
                     /gene="ulaF"
                     /locus_tag="ACHKAY_004615"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO5703159.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4685..4960)
                     /gene="yjfY"
                     /locus_tag="ACHKAY_004616"
     CDS             complement(4685..4960)
                     /gene="yjfY"
                     /locus_tag="ACHKAY_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO5703160.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5287..5682
                     /gene="rpsF"
                     /locus_tag="ACHKAY_004617"
     CDS             5287..5682
                     /gene="rpsF"
                     /locus_tag="ACHKAY_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO5703161.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5689..6003
                     /gene="priB"
                     /locus_tag="ACHKAY_004618"
     CDS             5689..6003
                     /gene="priB"
                     /locus_tag="ACHKAY_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO5703162.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6008..6235
                     /gene="rpsR"
                     /locus_tag="ACHKAY_004619"
     CDS             6008..6235
                     /gene="rpsR"
                     /locus_tag="ACHKAY_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO5703163.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6277..6726
                     /gene="rplI"
                     /locus_tag="ACHKAY_004620"
     CDS             6277..6726
                     /gene="rplI"
                     /locus_tag="ACHKAY_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO5703164.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6797..7591)
                     /gene="yjfZ"
                     /locus_tag="ACHKAY_004621"
     CDS             complement(6797..7591)
                     /gene="yjfZ"
                     /locus_tag="ACHKAY_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO5703165.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            7863..8156
                     /locus_tag="ACHKAY_004622"
                     /pseudo
     CDS             7863..8156
                     /locus_tag="ACHKAY_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(8220..8645)
                     /gene="tnpA"
                     /locus_tag="ACHKAY_004623"
                     /pseudo
     CDS             complement(8220..8645)
                     /gene="tnpA"
                     /locus_tag="ACHKAY_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2142 a         2055 c         2315 g         2194 t
ORIGIN      
        1 aatcccactc tatccgtgtg gaattatttg cgggtcgcgt cacatttaat cataaataat
       61 cttgttgtga ttacttttga aaattagagt gagtgcacaa cattccgggt gtgcggaata
      121 cccggttacc tcttcttcag gagatcgtta tggagatcct ctacaacatc tttaccgtgt
      181 tttttaacca ggtcatgacc aatgccccgt tgttgctggg tattgtgacc tgtctgggct
      241 acatcctact gcgcaaaagt gtcagcgtta ttattaaagg cacgattaaa accataattg
      301 gtttcatgtt gttgcaggca gggtccggca tcctcaccag caccttcaaa ccggtggtgg
      361 cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc cgatacttac gcgtcgatga
      421 tggcaaccat cgaccgcatg ggcgatgcct atagctgggt aggttacgcg gtgttgttag
      481 cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat taccggtatt cgtaccatca
      541 tgctgactgg ttatatcatg ttccagcagg cggggctgat tgccgttacg ttgtttattt
      601 tcggctactc catgtggaca accattatct gcacggcgat tctggtttcg ctctactggg
      661 gcattacctc caacatgatg tacaagccga ctcaggaagt gacggacggc tgcggtttct
      721 ccatcggtca ccagcaacag tttgcatcat ggattgccta taaagttgcg ccgttcctcg
      781 gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg ctggctgaat attttccacg
      841 acaacatcgt ctccacggcg attgtgatga ccatcttctt tggtgccatt ctgctctcct
      901 tcggtatcga caccgtgcag gcgatggcag gcaaagtgaa ctggacggtg tacatcctgc
      961 aaactggttt ctccttcgcg gtggcgatct tcatcatcac tcagggcgtg cgcatgtttg
     1021 tggcggaact ctctgaagca tttaacggta tctcccagcg cctgatccct ggtgcggttc
     1081 tggcgattga ctgtgcggct atctatagct tcgcgccgaa cgccgtggtt tggggcttta
     1141 tgtggggcac catcggtcag ctgattgcgg ttggcatcct ggtcgcctgc ggttcctcga
     1201 tcctgattat tcccggcttt atcccgatgt tcttctccaa cgccaccatc ggcgtgttcg
     1261 ctaaccactt cggcggctgg cgtgcggcgc tgaagatatg tctggtgatg gggatgattg
     1321 aaatctttgg ctgcgtctgg gcggtgaaac tcaccggtat gagcgcctgg atgggcatgg
     1381 cggactggtc gattctggca ccgccgatga tgcagggctt cttctccatc ggtatcgcct
     1441 ttatggccgt catcattgta attgcactgg cttatatgtt cttcgctggc cgcgcactgc
     1501 gcgcagaaga agatgcagaa aaacaactgg cagaacagtc tgcttaataa ggagttttga
     1561 ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca aggcagttcc atgatcatga
     1621 agatgaaagt ggaccagttt ttaacccaat caaacattga ccacacggta aatagctgcg
     1681 cggttggcga atacaaaagc gagttgagcg gcgcggatat catcatcgct tctacgcaca
     1741 ttgcgggcga aatcaccgtg accggcaaca aatacgtggt tggcgtgcgc aacatgctct
     1801 ctcctgccga ctttggcccg aaactgctgg aagtgatcaa agcgcatttc ccgcaggatg
     1861 tgaagtaagg acgcgccatg aaattacgtg attcgctggc ggagaataaa tccatccgcc
     1921 tgcaggctga agctgagaca tggcaggacg cagtaaaaat cggcgttgac ctgctggtgg
     1981 cggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt gaacagcacg
     2041 gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg gaagagggcg
     2101 ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc aaccacgaag
     2161 ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac acgcaccagg
     2221 aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc gaccgtttac
     2281 gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac gcggcagctt
     2341 aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa ccagactatg
     2401 gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat cgaagtgggt
     2461 accattctgt gcgtgggcga aggcgtgcgt gcggttcgtg acctgaaagc gctctacccg
     2521 cacaaaatcg tactggcaga cgctaaaatt gctgatgcgg gcaaaatcct ttcgcgtatg
     2581 tgcttcgaag ccaacgctga ctgggtgacg gtaatttgct gtgcggatat caacaccgcc
     2641 aaaggcgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga attgaccggc
     2701 tactggacct gggaacaggc gcagcagtgg cgtgacgcag gcattcagca ggtggtttat
     2761 caccgcagcc gtgacgcgca ggccgcaggc gtagcgtggg gcgaagcgga cattaccgcg
     2821 atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct ggcgctggaa
     2881 gatctgccgc tgttcaaagg tattccgatt catgtgttta tcgctggtcg cagcatccgt
     2941 gatgccgctt ctccggttga agctgcacgt cagttcaaac gttccatcgc cgaactgtgg
     3001 ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga aaaagcgctc
     3061 cccgccgggg agtgctggct ggaacgcctg caactggcaa aaacgttggg cttcgatttt
     3121 gtcgagatgt cggtagatga aacggacgag cgcctgtcgc gcctcgactg gagccgcgag
     3181 cagcgtctgg cgctggtgaa tgcgattgtt gaaaccggcg tgcgcgtgcc gtccatgtgc
     3241 ctttctgctc atcgtcgttt cccgctgggc agtgaagatg acgcggtgcg ggcgcagggg
     3301 ctggagatta tgcgtaaagc tatccagttc gcccaggacg tcggcattcg cgtgatccag
     3361 ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg tcgtttccgt
     3421 gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct ggcgatggag
     3481 atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc gcactatctc
     3541 aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg ggacaacgat
     3601 gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtacatgt gaaagacacc
     3661 aaacctggcg tcttcaaaaa cgtgccgttt ggcgaaggtg tagtggattt cgaacgttgt
     3721 ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat gtggagcgaa
     3781 acggcggaag acccggcggc agaagtggcg aaagcgcgtg attgggtgaa agcgcgcatg
     3841 gcgaaagcgg gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtatttgaag
     3901 ccaacatgga tctgccgcgc tacgggctgg tgacctttac ctggggcaac gtcagcgcta
     3961 tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgtcgcctac gaaaccatga
     4021 aagcagacga tatggtggtg gtcgatatga gcggtaaggt ggtggaaggg gagtatcgcc
     4081 catcttccga caccgcgacg catcttgaac tctaccgtcg ttatccgtcg cttggcggca
     4141 ttgtccatac ccactccact catgctaccg catgggcgca ggcggggctg gcgatcccgg
     4201 cgttaggcac cacgcacgct gactacttct ttggtgacat cccgtgtacg cgtggattaa
     4261 gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg caaagtgatt atcgaaacgc
     4321 tgggcaacgc cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg
     4381 cctgggggaa agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga
     4441 aaatggcgtg gattgcgcgc agtattaatc cacaactcaa tcacatcgac agcttcctga
     4501 tgaataaaca cttcatgcgt aagcatggtc ctaacgctta ttacgggcag aagtaaaaca
     4561 cgcgctgcgg aaatttcctt cttcgggaga taactggtct aattccgcag ccgtttttca
     4621 aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct
     4681 ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca
     4741 tcagaattac gtggtagtac gttgcgccct gcgcatctgt ttcttcattt aatgcctgac
     4801 gtgcttcact ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat
     4861 cgtccatatt tctggcctgg tggttattga tttcaatggc tgcccatgta tttgcactta
     4921 gcaaaagcac agccagaagg gctaaaacac gactgaacat agatacctcc tcaacggctg
     4981 actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag
     5041 ttgatggtga ccatttctga tgcagttgtt cgaaaaaaca ctgtgacgga atgtgatgaa
     5101 cttcaaatca gggagttagt tcttttgagc gaaagatgag atttatttgt cggctggcct
     5161 tgagtttagc ggggcataca agtactataa cgcgtcattt ttcagccgac ctttaacacg
     5221 ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc
     5281 aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt
     5341 ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt
     5401 ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac
     5461 tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc
     5521 cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa
     5581 gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac
     5641 gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt
     5701 ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt
     5761 cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg
     5821 caggcatggt gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac
     5881 agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag
     5941 aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac
     6001 tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt
     6061 tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa
     6121 gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc
     6181 tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt
     6241 cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa
     6301 gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac
     6361 ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa
     6421 gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc
     6481 gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt
     6541 aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt
     6601 gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac
     6661 gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct
     6721 gaataattcg ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc
     6781 tatttttcgt caggtattag tttcgcaagt agatccattc accattctct tgtgtccata
     6841 agtcaggatg gtttgctggc gttagatgca tacgatcact ccttatgtct cggtcctgcg
     6901 gcactcgctc gaaacctaaa cccgagtaga ttgctgctga atttgataag gaacgtagac
     6961 caattttcaa atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc
     7021 cgagggcatt agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg
     7081 gtagttcaga tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg
     7141 tttggccggt atagtctgga gtaccataac caggtcccaa aaccacttcg tgtattgtgt
     7201 atttactgtt gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta
     7261 acctcgcacc cgcgtgagct ttattgtatt tctgggcgtg aatattatct aatgtagctt
     7321 tatggttcca tccaaaagaa ttatcaccat tcattgttac tccatcgtaa gcattacact
     7381 tcgttgctaa cttattgaat gcttgccagt cagagagatc tagtttgtgg atctcaacag
     7441 tacgtctgcc atcgcaatta tgatctcttc cacgatttgg cagtaatgta gcacgtgcat
     7501 attgatgaaa ttttaatttt actggtttgt cggtggacga aaaatggggt gaatatgcag
     7561 gaaatgaata aatagtcgtt ggtaatgtca ttgtaatgtt acctgtttaa tgtttttata
     7621 aatatcgcag ctgatgttca cagaaaaaga atcgatatta ataaaaataa gtttgttttc
     7681 tgaagtagtg aaaatagcgg atggtgaaag atattaatat caaaaatcgc tcattatgga
     7741 tataacgatg cccccgggga gaaaatgact tccacgcctt ccaccataca ttcttgttgt
     7801 gcggtatgta attattctac gctgttggta tcggtgcgtt tgttaatcat ctggttaacc
     7861 agccacaaaa agccattgag aaaattattg attttacatg gggttattat attgctaacc
     7921 cttggttttt aaaaattgtg catagtgaaa atcaaagtaa aggtgtgcat tatgcaaaat
     7981 cacagcgtct cttagaaatt aaccatgcgc acttgcagtt aatggaatcc ttattggatg
     8041 aagggaagaa atacaatatt tttaagccag gtatcgaccc attgcaggtg aatattaata
     8101 ttgctgcgct tgggggatat tatttgatca accagcatac gcttggcctg gtttatctgg
     8161 tgcgccgtaa aaccccgtcc ttcagggcgg ggatataagg cgcggttttc cacctgacca
     8221 ggtgtttgct gttgttcaat gtattggcgg atgacggata ttggcgcacc tccgcagcta
     8281 ttggcaaaat agccaggact ccacaaaacg cctttgtagt aatacctgac tgcaatatct
     8341 ggtcgatctc gtcgcagtaa cctacccgat acgcctttga ggctgtttac cagactggat
     8401 atcgccagtt tgggaggata gctaattaac aaatggacgt gatctggttc gccatccatt
     8461 tcaaccagtt cagcttcaaa atcagcacat acatttgaaa agtaagtgcg tagtttttct
     8521 gtcgcgtcat ggtcaaaaat ctggcgtcgg tatttggtga caaagaccag gtgaacatgc
     8581 atcaggaaaa cacaatgcct tccacgccgg atatcggttt cttttttcac agaccaaaat
     8641 ataatttgac ctgtggaacg actacaggca tttaaattcc agttaagacc cggtggtcaa
     8701 caggag
//
LOCUS       DAZFKM010000181         8678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFKM010000181 DAZFKM010000000
VERSION     DAZFKM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..696)
                     /gene="entD"
                     /locus_tag="ACHKAY_004624"
     CDS             complement(76..696)
                     /gene="entD"
                     /locus_tag="ACHKAY_004624"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO5703166.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(733..831)
                     /locus_tag="ACHKAY_004625"
                     /pseudo
     CDS             complement(733..831)
                     /locus_tag="ACHKAY_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(871..3111)
                     /gene="fepA"
                     /locus_tag="ACHKAY_004626"
     CDS             complement(871..3111)
                     /gene="fepA"
                     /locus_tag="ACHKAY_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO5703167.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3354..4555
                     /gene="fes"
                     /locus_tag="ACHKAY_004627"
                     /pseudo
     CDS             3354..4555
                     /gene="fes"
                     /locus_tag="ACHKAY_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            4558..4776
                     /gene="ybdZ"
                     /locus_tag="ACHKAY_004628"
     CDS             4558..4776
                     /gene="ybdZ"
                     /locus_tag="ACHKAY_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO5703168.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            4773..8654
                     /gene="entF"
                     /locus_tag="ACHKAY_004629"
     CDS             4773..8654
                     /gene="entF"
                     /locus_tag="ACHKAY_004629"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO5703169.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         1866 a         2330 c         2400 g         2082 t
ORIGIN      
        1 gtggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgaatcgt
       61 accagatgtt gtcaattaat cgtgttggca cagagttatg actatttttt cttttatctg
      121 ccagtgcaca gcaaacatct cattctcacg atgaatgatg acctgctgtt tattccagct
      181 aattatctga tagtccagaa aacctgcatc agtttggatc tcacttgcct taaatgcgct
      241 ctctttggcg gaaaatgcca gtgtcagcgc cagagaaaag gctaaaccgc agtctgcgag
      301 tcgctcgtgt tctgctggtg taataatgtt gtctgtcaat tcggtcgctg tttgcgcaga
      361 aaaaatttct tcgatatcaa cgccaatcgg ttgacgggaa accacggcca atgccgtagc
      421 cccgcagtgg ctaatactgc cgtatacctc cgcaggccac accggttggc gtagctcgcc
      481 gattgcgggc acacatttat agccatattc ccgcagcgca taaattgcag cgatccgccc
      541 ggctaaatgt tcggctttgc gcttccgtcc agcgtgttgc agttgcgcgt aatgcggcag
      601 ccagagtaaa tcctgctcac aaaaattcgc cggatcgaac tcaacaaaat gcagcgtatg
      661 tccggcaaag gggagggagg tatgcgtagt tttcatgtcg accatattag agactgatga
      721 caaacgctaa actgcctgat gcgctacgct tatcaggcct acatggtcct gcaatatatt
      781 gaattggcaa gatttttgta ggccggataa ggcgttcacg ccgcatccgg catgaacgac
      841 gcgcactttg tcaacaatct gacgttagca tcagaagtga gtgtttacgc tcatgtacca
      901 cgtacgtccc ggctcgttat aggtatacgc cccggcaccc gcgatatagt tggcccctgc
      961 cagatcgccc gtggtctggg cattacccgc acgccacaaa cgtttgtcga acaggttgtc
     1021 cacgccgccg gtcagactga cattcttcgt cacatcccag gtcgcgctca gacccacaat
     1081 gctgtaagga ctgatttctt tggtttccgg gccaaccgct ggctgacctt tgtagttgta
     1141 ttttttcggc tgctgcttac cgtaccaggt gaaggtcgtt tgcatcgaca aatcttcccg
     1201 cgcctgccag ctcagcgttg agttcaacgt gtactccggg atgatcgaca aacggtcgcc
     1261 cgtggttttg ttttcactct tcagcatata agtgatgtta ttggtccaca tcaccgtttc
     1321 gctaactggt acgtttaacg atccttccag accttcaacc actgctttcg gcacgttatc
     1381 ccactgatag agatcggtgc cgactgcgtt ttgccctaca gccacatagc ccgcttcaat
     1441 cttattgcga taatcgttac ggaaccaggt gacgcccgcc agccagccgt cgcgtttgaa
     1501 ctccagacca atctctttgt tgatgctggt ttctgctttc aggtcatcgt taccttgcag
     1561 atagcagccg cccgcgctgg catagcagcc ctgaccttta ctgtagagaa tgtagttcgg
     1621 gttagtctgg tacaggctcg gtgctttata agctcgggca atgcccattt tgagcgtgaa
     1681 gtcatcgcct aaaccttgcg atatgttcag cgccgggctc cagttattgc cgacaatact
     1741 gtgatgatcg aaacgcagcc ccggcgttac gatggtgctg tcagtcagct ccatgttgtt
     1801 ttcggcaaac agcgagaaaa tttctgcttt tgaatacggg ctacggtcgg tggcactcat
     1861 accatcaata gcgccgccgg tgttcgcccc ggtcagcgcc tgggtattgg aactcaagtc
     1921 cttcatccgt tgctgattcc actccgtacc cagcgtcagc gtctggttaa cgaggatatc
     1981 aatcggcagg ttaacttcgc tgtgcagcat cacgtcatcg agatcgatat cgacgaaatc
     2041 ctgtgtcgct ttttcgttaa atttcccttc ggtaccgccc gccagacctt ccggaatacg
     2101 cgagttacgg gtgtgttcat actgcaccca gttgctggtg gtcacgccgt tatcccagcc
     2161 accgttccag gtcagcgcgt agttctggcg atacaggcgg ttggtttcat cgccatattt
     2221 cgagcgagtg taagcgtcag agttggtgtt ctgggtatcg cccgcataca ggttaccctg
     2281 gcggctgtaa cccgcttcca gttccagcga ttgcaatggt gcgaagtccc agcgcaccac
     2341 gccgttaata tctttattga tcaccccttc gcgcccggct ggtaacgtcg tggcatacgt
     2401 tccggcacgc gcggactgat ggccctggtt gatatcccac gcgtcagcct gggttttgtc
     2461 gaggttgcca tacaagcgga agctgaattc gtcgcccagc ggaccggtca ggctgaagtt
     2521 ggtgcgttta gtggctcctt cctctttatg ttctggtgca ttgaaataag cgtcccagga
     2581 gccgtgccac tcaccgctgc cttttttggt aatgatgtta accacgccgc ccgccgcgcc
     2641 gttgccataa cgcgcagctg ccggaccacg cagaacttca atacgttcaa tcatttcagg
     2701 tggcacccag gaggtatcac cacgggtatc gcgctcgcca cgccagccct gacgcaccga
     2761 gttacggctg cttaccggct tgccgtcaat caaaatcagc gtgttttccg gacccatacc
     2821 gcgaatatca atctgtcggt tattcccacg ctgaccactg gtggagttac cggtcaggtt
     2881 aacgcctggc atggtacgga tgatctccga cacatcgcgg gcaacggggt ttttgcggat
     2941 ttcatctgcg gtgatggtcg aaacgccagg cgcctgtaag ttctgctcgg cggcggtaac
     3001 gacaatggta tcgtcatgtg aaacaggagt atcggtcggc tcttgtgcct gcgctacccc
     3061 ataaatcccc agattgacca acaaggccag ggaatgaatc ttcttgttca ttgttttatt
     3121 cctgcatttt tgccacgaat tgcaactgtc gggcatggtc gtcctcaaca cgacgcatcc
     3181 cgctaccgcg aaaacctttg atcctgaaag acacgcagtg cagttggtta ataaatgtcc
     3241 gcgcttccca cggcgcgcca ttacgctatt gcaaatgcaa atagttatca ataatattat
     3301 caatatattt ctgcaatcaa tgaaaaattg cacagtaaac atggggttat ggtgtgacgg
     3361 cgttaaaagt aggaagtgag agctggtggc agtcgaaaca tggcccggaa tggcagcgtc
     3421 tgaatgacga aatgtttgag gtcactttct ggtggcgtga tccccaaggt tctgaagaat
     3481 actcgacgat aaagcgcgta tggatctaca tcactggtgt gaccgatcat catcagaaca
     3541 gccagcccca gtcgatgcag cgaattgcag gtactgatgt ctggcagtgg acgacacaac
     3601 tcaatgccaa ctggcgcggc agctactgct ttattcccac cgaacgcgat gacatttttt
     3661 ctgcaccatc ccccgatcgc ctcgaattac gtgaaggctg gcgaaaacta ttaccccagg
     3721 caatagccga tccgctgaat ccacaaagct ggaaaggcgg gcgagggcac gctgtttctg
     3781 cactcgaaat gccgcaagcg cctctgcaac cgggatggga ttgtccgcaa gcgccagaaa
     3841 tagcagccaa agaaattatc tggaaaagtg aacggttgaa aaattcacgt cgtgtatgga
     3901 tttttaccac cggcgatgca acagcagaag aacgtccgct ggcagttttg ctcgatggcg
     3961 aattttgggc gcaaagtatg cccgtctggc ctgtgctgac ttcgctgacc catcgtcagc
     4021 aacttcctcc cgccgtgtat gtgttgatcg acgctatcga caccacgcac cgcgcccacg
     4081 aactgccgtg taatgcggat ttctggctgg cagtacagca agagttatta cccctggtga
     4141 aagctattgc cccttttagc gatcgcgccg atcgcaccgt ggtcgccggg cagagttttg
     4201 gtgggctgtc cgcgctgtat gccggactgc actggcctga acgctttggc tgtgtattaa
     4261 gccagtcagg atcgtactgg tggccgcatc ggggcgggca gcaagagggc gtgttacttg
     4321 aaaagcttaa agctggtgaa gttagcgccg aaggtctgcg cattgtgctg gaagcgggta
     4381 ttcgcgagcc gatgattatg cgggccaatc aggcgctgta tgcgcaatta caccccataa
     4441 aagaatccat tttctggcgt caggttgacg gcggacatga tgcgctttgt tggcgcggtg
     4501 gcttgatgca ggggctaatc gacctctggc aaccactttc catgacagga gttgaatatg
     4561 gcattcagta atcccttcga tgatccacag ggagcgtttt acattttgcg caatgcgcag
     4621 gggcaattca gtctgtggcc gcaacaatgc gccttaccgg caggctggga cattgtgtgt
     4681 cagccgcagt cacaggcgtc ctgccagcag tggctggaag tccactggcg tactctgaca
     4741 ccggccaatt tcacccagtt gcaggaggca caatgagcca gcatttacct ttggtcgccg
     4801 cacagcccgg catctggatg gcagaaaaac tgtcggattt accctccgcc tggagcgtgg
     4861 cgcattacgt tgagttaact ggagatgttg atgcgccatt actggcccgc gcggtggttg
     4921 tcggactagc gcaagcagat acgttgcgga tgcgttttac ggaagataac ggcgaagtct
     4981 ggcagtgggt cgatgatgcg ctgatattcg aactgccaga aattatcgac ctgcgaacca
     5041 atattgatcc gcacggtact gcgcaggcat taatgctggc ggatttgcaa caagatttgc
     5101 gcgtcgatag cggtaaacca ctggtctttc atcagctgat acatgttgcg gataaccgct
     5161 ggtactggta tcagcgttat caccatttgc tggtcgatgg cttcagtttc ccggccatta
     5221 ctcgccagat cgccaatatt tactgcacat ggctgcgtgg cgaaccaacg cctgcttcgc
     5281 cgtttacgcc tttcgctgat gtagtggaag agtaccagca ataccgcgaa agcgaagcct
     5341 ggcagcgtga tgcggcattc tgggcagaac agcgtcgtca actgccgccg cccgcgtcac
     5401 tttctccggc acctttagcg gggcgcagcg cttcggcaga tattctgcgc ctgaaactgg
     5461 aatttaccga cggggaattc cgccagctgg ctacgcaact ttcaggtgtg cagcgtaccg
     5521 atttagccct tgcgctggca gccttgtggc tggggcgatt gtgcaatcgc atggactatg
     5581 ccgccggatt tatctttatg cgtcgactgg gctcggcggc gctgacggct agcggacccg
     5641 tgctcaacgt tttaccgttg ggtattcaca ttgcggcgca agaaacgctg ccggaactgg
     5701 caacccgact ggcagcacaa ctgaaaaaaa tgcgtcgtca tcaacgttac gatgccgaac
     5761 aaattgtccg tgacagcggg cgagcggcag gagaggaacc gctgttcggt ccggtactca
     5821 atatcaaggt atttgattac caactggata ttcctggtgt tcagacgcaa acccataccc
     5881 tggcaaccgg tccggttaat gaccttgaac tggccctgtt cccggatgaa cacggtgatt
     5941 tgagtattga gatcctcgcc aataaacagc gttacgatga gccaacgtta atccagcatg
     6001 ctgaacgcct gaaaatgctg attgcccagt tcgccgcgga tccggcgctg ttgtgcggcg
     6061 atgtcgatat tatgctgcca ggtgagtatg cgcagctggc gcagatcaac gccactcagg
     6121 ttgagattcc agaaaccacg ctgagcgcgc tggtggcaga acaagcggca aaaacaccgg
     6181 atgctccggc gctggcagat gcgcgttacc tgttcagcta tcgggaaatg cgcgagcagg
     6241 tggtggcgct ggcgaatctg ctgcgtgagc gcggcgttaa accaggggac agcgtggcgg
     6301 tggcactacc gcgctcggtc tttttgaccc tggcactcca tgcaatagtt gaagctggcg
     6361 cggcctggct accgctggat accggctatc cggacgatcg cctgaaaatg atgctggaag
     6421 atgcgcgtcc gtcgctgtta attaccaccg acgatcaact gccgcgcttt agcgatgttc
     6481 ccaatttaac aagcctttgc tataacgccc cgcttacccc ccagggcagt gcgccgctgc
     6541 aactttcaca accgcaccac acggcttata tcatctttac ctctggctcc accggcaggc
     6601 cgaaaggggt aatggtcggg cagacggcta tcgttaaccg cctgttatgg atgcaaaatc
     6661 actacccgct tacaggcgaa gatgtcgttg cccaaaaaac gccgtgcagt tttgatgtct
     6721 cggtgtggga gtttttctgg ccgtttatcg caggggcaaa actggtgatg gctgaaccgg
     6781 aagcgcaccg tgacccgctc gctatgcagc aattctttgc cgaatatggc gtaacgacca
     6841 cgcactttgt gccgtcgatg ctggcggcat ttgttgcatc gctgacgccg caaaccgctc
     6901 gccagaattg cgcgacgttg aaacaggttt tctgtagtgg tgaggcctta ccggctgatt
     6961 tatgccgcga atggcaacag ttaaccggcg cgccgttgca taacctgtat ggcccgacgg
     7021 aagcggcggt agatgtcagc tggtatccgg cttttggcga ggaactggca caggtgcgcg
     7081 gcagcagtgt gccgattggt tatccggtat ggaatacggg tctgcgtatt cttgatgcga
     7141 tgatgcatcc ggtgccgccg ggtgtggcgg gtgatctcta tctcaccggc atccaactgg
     7201 cgcaggggta tcttggacga cccgatctga ccgccagccg ttttattgcc gatccttttg
     7261 ccccaggtga acggatgtat cgtaccggag acgtcgcccg ctggctggat aacggcgcgg
     7321 tggagtacct cgggcgcagt gacgatcagc taaaaattcg cgggcagcgt atcgaactgg
     7381 gcgaaatcga tcgcgtgatg caggcgctgc cggatgtcga acaggcagtt acccacgcct
     7441 gtgtgattaa ccaggcggca gccaccggtg gtgatgcgcg tcagttggtg ggctatctgg
     7501 tgtcgcaatc gggactgccg ttggatacca gcgcattaca ggcacagctt cgcgaaacat
     7561 tgccgccgca tatggtgccg gtcgttctgc tgcaacttcc gcagttgccg cttagcgcca
     7621 acggcaagct ggatcgcaaa gccttaccgt tgcctgaact gaagacacaa gcgtcgggac
     7681 gtgcaccgaa agcgggcagt gaaacgatta tcgccgcggc attcgcgtcg ttgctgggtt
     7741 gtgacgtgca ggatgccgat gctgatttct ttgcgctggg cggtcattcg ctactggcaa
     7801 tgaaactagc agcgcagtta agtcgacagt ttgctcgtca ggtgacgccg gggcaggtga
     7861 tggtcgcgtc aaccgtcgcc aaactggcaa cgattattga tggtgaagag gacagctccc
     7921 ggcgcatggg attcgaaacc attctgccgt tgcgtgaagg taatggcccg acgctgtttt
     7981 gtttccatcc ggcgtccggt tttgcctggc agtttagcgt gctctcgcgt tatctcgatc
     8041 cacaatggtc gattattggc attcagtcgc cgcgccctca tggacccatg cagacagcga
     8101 caaacctgga tgaagtctgc gaagcgcatc tggcaacgtt acttgagcaa caaccgcacg
     8161 gcccttatta cctgctgggg tattcccttg gcggtacgct ggcgcagggt attgcggcgc
     8221 gactgcgtgc ccgtggcgaa caggtggcat ttcttggttt gctggatacc tggccgccag
     8281 agacgcaaaa ctggcaggaa aaagaggcta atggtctgga cccggaagtg ctggcggaga
     8341 ttaaccgcga acgcgaggcc ttcctggcgg cacagcaggg aagtacttca acggagttgt
     8401 ttaccaccat tgaaggcaac tacgctgatg ctgtgcgttt gttaaccacc gcccatagcg
     8461 ttccgtttga tggaaaagca acgttatttg ttgctgagcg cacacttcag gaaggtatga
     8521 gtcccgaacg tgcctggtcg ccgtggattg cggagctgga tatctatcgt caggattgtg
     8581 cgcatgtgga tattatctct ccaggggcgt ttgtaaaaat tgggccgatt attcgcgcaa
     8641 cgctaaacag gtaaattaat aatatctata aacccata
//
LOCUS       DAZFKM010000182         8605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFKM010000182 DAZFKM010000000
VERSION     DAZFKM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(121..197)
                     /locus_tag="ACHKAY_004630"
     tRNA            complement(121..197)
                     /locus_tag="ACHKAY_004630"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Asp,seq:gtc)
     gene            complement(330..1061)
                     /gene="dnaQ"
                     /locus_tag="ACHKAY_004631"
     CDS             complement(330..1061)
                     /gene="dnaQ"
                     /locus_tag="ACHKAY_004631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO5703170.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1126..1593
                     /gene="rnhA"
                     /locus_tag="ACHKAY_004632"
     CDS             1126..1593
                     /gene="rnhA"
                     /locus_tag="ACHKAY_004632"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO5703171.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1590..2312)
                     /locus_tag="ACHKAY_004633"
     CDS             complement(1590..2312)
                     /locus_tag="ACHKAY_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5703172.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2346..3101
                     /gene="gloB"
                     /locus_tag="ACHKAY_004634"
     CDS             2346..3101
                     /gene="gloB"
                     /locus_tag="ACHKAY_004634"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO5703173.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3173..4531
                     /gene="mltD"
                     /locus_tag="ACHKAY_004635"
     CDS             3173..4531
                     /gene="mltD"
                     /locus_tag="ACHKAY_004635"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO5703174.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4579..5349)
                     /locus_tag="ACHKAY_004636"
     CDS             complement(4579..5349)
                     /locus_tag="ACHKAY_004636"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5703175.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5427..6227)
                     /locus_tag="ACHKAY_004637"
     CDS             complement(5427..6227)
                     /locus_tag="ACHKAY_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO5703176.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6468..7382
                     /gene="yafC"
                     /locus_tag="ACHKAY_004638"
     CDS             6468..7382
                     /gene="yafC"
                     /locus_tag="ACHKAY_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO5703177.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7379..8182)
                     /gene="dkgB"
                     /locus_tag="ACHKAY_004639"
     CDS             complement(7379..8182)
                     /gene="dkgB"
                     /locus_tag="ACHKAY_004639"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO5703178.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8345..8421)
                     /locus_tag="ACHKAY_004640"
     tRNA            complement(8345..8421)
                     /locus_tag="ACHKAY_004640"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8385..8387),aa:Asp,seq:gtc)
BASE COUNT         2292 a         2066 c         2078 g         2169 t
ORIGIN      
        1 gattaacaca tttacagcat aacaaaacca tagaccgaag aaatagcggg atttaaatgc
       61 aaaaaaaacc gcaaaattaa aaatcttgcg gttctctgaa ctcattttca tgagtgaatt
      121 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
      181 gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt acggattgcc
      241 tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa atcgcccaag
      301 ccgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcagct tccgcccttc
      361 ttctccacca gatcaagacg ggcttcatga gctgcaagct cttcatctgt cgcaaaaaca
      421 acgcgtaact tacttgcctg acgtacaatg cgctgaattg ttgcttcacc ttgttgctgc
      481 tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat cgccagataa
      541 acttccgcaa ggatctgtgc atcgagtaat gccccgtgta gcgttcgttt actgttatct
      601 atttcgtagc gggcacataa cgcatcgagg ctgttgcgct taccaggaaa cattttcctc
      661 gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat atcgcgctta
      721 agcaacgaaa actcgtagtc cataaagccg atatcgaacg ctgcgttatg gatcaccaac
      781 tccgcgccgc ggatatagtc catgaactca tcggctactt cggcaaacgt gggcttatcg
      841 agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac cagccgatcg
      901 ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac ttcaacggca
      961 ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat accggtggtt
     1021 tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat ttatgtcaga
     1081 cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct taaacaggta
     1141 gaaattttca ccgatggttc gtgtctgggt aatcctggac ctgggggtta cggcgctatt
     1201 ttacgctatc gcggacgcga gaaaaccttt agcgcaggct acacccgcac caccaacaac
     1261 cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca ttgcgaagtc
     1321 attttgagta ccgacagcca gtatgtccgc caaggtatca cccagtggat ccataactgg
     1381 aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa aaaatgtcga tctctggcaa
     1441 cgtcttgatg ctgcattggg gcagcatcaa atcaaatggg aatgggttaa aggccatgcc
     1501 ggacacccgg aaaacgaacg ctgtgatgaa ctggcccgtg ctgcggcaat gaatcccaca
     1561 ctggaagata caggctatca agtcgaagtt taagcctgtg gtttacgata ttgccgggtg
     1621 gctccaaccg cctggcgaat tcgtggcttg tttttactct gtttcatcgg atttagcgtt
     1681 aaaggaatag tccgtttccg ggcaacaata agttgtaagc aaccaagcgc aggaatatgt
     1741 gcattcaata gttttccacc gtgtttgttc cacgggagaa cgtggaaacg gctggcgtgc
     1801 agcacttcaa aattcaacaa agagagccag tccagttgcc gcatcagagt aaacatccgg
     1861 ctgttatagg gcgaggtttt tcgcaatacc ggcacaagtt tgcgtaatcc cattaaacta
     1921 atgggattga agccactaat caccagccag ccatcatcaa tcaatacccg atcggtttca
     1981 cgcaataaac gatgcggatc ggtgcaccac ggcaatgtat gtgccagtag acaaacatca
     2041 acggatttat cggcaaaagg aagatgaagt ggatccgcct gcacctggac gggcattcct
     2101 tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat ttctgcgctt
     2161 aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg gttgagctgg
     2221 cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagcg atcaggagcc
     2281 acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac actaagaggt
     2341 aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg gttttgaatg
     2401 atgaagcagg tcgctgcctg attgtcgatc ccggagacgc agagccagta ttaaacgcca
     2461 ttgccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcat cacgatcacg
     2521 ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat ggtccacaag
     2581 agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc ttcgttttgg
     2641 ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc tgttacttca
     2701 gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt aggttgtttg
     2761 aagggacagc atcacaaatg tatcaatcac ttaataagtt aagtgcgtta cctgacgata
     2821 cattggtatg ttgtgctcat gaatatacgt tatcaaatat gaagtttgct ttgagtattc
     2881 ttccgcacga tttgtccata aatgattatt atcgtaaagt taaggagtta cgggcaaaaa
     2941 atcaaataac actacccgta attctgaaaa atgagcggca aattaatgtt tttttaagaa
     3001 cggaagatat tgatttaatt aatgtaatta atgaagaaac attattgcaa caacctgaag
     3061 agcgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt cttgtcatcg
     3121 gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac acatgaaggc
     3181 aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg gcaacgttca
     3241 acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt ttacaagtca
     3301 ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt gggctttcat
     3361 tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac agaaacagaa
     3421 atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc cgtatatgta
     3481 ctggatagca gggcaagtta aaaaacgtaa catgcctatg gaactggtac tactacccat
     3541 agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag gcatctggca
     3601 gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt atgacgcgcg
     3661 tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc tgaacaagat
     3721 gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag gtcgagtcat
     3781 gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt cgttaccgtt
     3841 gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata ttctcaaaaa
     3901 cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc tggcgcgtgt
     3961 gcacctgagc agcccggttg aaatggcgaa ggttgcagat atggcgggga tttccgtcag
     4021 caagctgaag acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa gtggtccgca
     4081 gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctgg cttcaggcga
     4141 aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca gccgtgttta
     4201 caccgtacgc tctggcgaca cgctttcaag tatcgcttca cgtctcggcg taagcaccaa
     4261 agatttgcag cagtggaaca aactgcgcgg atctaaactg aagccaggcc aaagtttgac
     4321 gattggcgca ggcagtagcg cacagcgact ggcaaacaac agcgatagca ttacgtatcg
     4381 tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga acatcaaaga
     4441 tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgataagc tgacgttgtt
     4501 tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaag gcaccgattc
     4561 ccccggtgcc ttttttattt atgccgcttt atgtgcttct accatgatga tatcactggt
     4621 gaaagagccg tcattctgca aggcaaaata cgttctcacc tctgtcgatg cgctctgttg
     4681 gtaaatgcga atagcgtcta ttaacgcttc tggcgtacgc attctcacga cccatgaaga
     4741 aaattccagc ggtaacttat ctgtaattaa attatcaact atcagattgg cttcattgat
     4801 taacgtcaac cactcaccgc tggcgtagtt tcgtacgtga gaggtatcgc gtaatgcttc
     4861 taccgtctgt aaccagatgt cgcgcactgg gtgacccgga gacattacgt ccatcacaat
     4921 cagcctaccg ccaggtttca atatccggtt cacttcacgc agtgctgcac caacatcatg
     4981 ccagtgatgg gcagaataac ggctgataac aatatcaaat gcgttgtcgg caaatggcag
     5041 actttcggca taaccctggc gggtggtgat atttttcagt tgccggactt cggcagcttg
     5101 tgccacgaca tctagcatgt gggcagataa gtcatacgcc accaccgtgc tcacgttttg
     5161 cgcagcgaca aagctggcat gccctgctcc gcagcccata tcaagtacac ttgcaccagg
     5221 ataatcagcc agacgcaccg ccaggcgctg taaatctcgt ccggatgcat gtacggtact
     5281 ggtcagatat tcgctggctt gagagctaaa ctgcttttct acatggtcat ggtgggattg
     5341 tattgtcatt gcgctgtcct tttgttattt gagtataaaa gggcagcgcc aaacgttggt
     5401 ctgccctgtg gcagacctga cataccttat ttatcaggct tgccgggact gaattcaacg
     5461 agtagcggat tgtgatcgga agcgcgcgta accagtacag aagcttcgct gacgttcaga
     5521 ccacggtaga aaacaaaatc gagcgggcga ccaaacgccc gacggcgctg atcatcggta
     5581 aaacgcacct ggcgcagcga catttcccgc gcaaagcgat ataacgcgtt catccttcta
     5641 cggctccagg cattgaaatc tcccgccata atgaccgggc cgctgtggtg agctatctga
     5701 tcaccaatag gaagtaactg cttactatag acatccacgc ccagactgaa gttgacggcg
     5761 tgtatattaa ccaccatcaa caggcgggtg tcaggtaatg gatagaccgt caccagtgcc
     5821 gacttcgcca gacgcaaaat gggttctcgt tcacgtaacg ggcagcaata cactggatgc
     5881 gccgccgaaa gggtcattac accggaagga tgttgtggca gcacgaaagc gggcacctga
     5941 tcggcggcaa gatagttagc ggtcgcaaac tgtactaact ctggcgttgt ctgcgcttcc
     6001 tgcaataaca ccagatgtgc atctttgccg tagttcttta ataccgacaa ccattcagcg
     6061 cgttgctgtt tgtatatgtt ccacaccagg atccgaatac gctcttcagt acttaacggt
     6121 tctccaggtg gtaatgcctg gccgatgctc gcaaaagacc ccggcggtaa gatcctttcc
     6181 gcaggttgtc cggcaacata gcgcatggca taggtgtttt ttcgcacttt tgactttgaa
     6241 acctctatta cgtcaaccct tccgcactcg ggagggttct actgttatag ggattttagc
     6301 tcactctttc aacgagcaat tgtatattgt tatgtaagca agtgcttaca caggtcagaa
     6361 ttttgctcat tattgagaac attaaatcgg atgacatatc acgcatcctg atggtttaat
     6421 gacaaataaa ttcaaaatat ctcttctatt ttgcaacagg agcaaaaatg aaagccacgt
     6481 cggaagaact cgccattttt gtttcggtcg tagaaagcgg cagctttagc cgggcagcgg
     6541 aacaattagg gcaagcaaac tcagcggtaa gccgggcagt gaaaaagctg gagatgaaac
     6601 ttggcgttag cctgcttaat cggaccacgc gacaacttag cctgacggaa gaaggccagc
     6661 gttattttcg tcgcgtacag tcaattttgc aggagatggc agcggcagaa tcagaaatta
     6721 tggagacgcg taatacaccg cgtggactgt tacgcatcga tgccgcaact ccagtggtgc
     6781 tgcactttct gatgccgtta attaagcctt tccgtgaacg ctatccggaa gttactttat
     6841 cgctagtctc ctccgaaacg attattaatt tgatcgaaag aaaagtggat gtcgcgatac
     6901 gcgctggtac gttaacggat tccagcttac gtgccaggcc gttatttaac agttatcgaa
     6961 aaattatcgc ctcccccgat tatatttccc gctacgggaa gccagaaacg atcgacgatt
     7021 taaagcaaca tgtttgcctg ggattcactg aacccgcttc cctcaatacc tggccgatag
     7081 cctgtagtga tggacaatta catgaggtga agtacggttt gtcatccaat agtggggaaa
     7141 cactgaaaca gctttgcctg agtggcaacg ggattgcgtg tttgtccgac tacatgatcg
     7201 acaaagaaat cgttcgcgga gaattggtgg agttaatggc agataaagtg ttgccagtgg
     7261 aaatgccatt cagtgcagtc tattacagcg accgtgcggt aagtacgcgc atccgggctt
     7321 ttatcgattt ccttagcgag catgtaaaaa cagctcccgg aggagctgtc agagaggctt
     7381 aatcccattc aggagccaga ccttccgggc taaccaggcg gtcgttgcaa tccagtgcgg
     7441 cgatcgcttt tttatcttcg gcatcaagct gtaaattttg tgccttaaga ttactttcca
     7501 ggttttcacg tttagtagaa gaaggaatta ctgagtaacc ttcccccata gcccacgcca
     7561 gaatcacttg tgccggagtc gcattgtgtt tagctgcgat acgagcaata acctcatctt
     7621 tcaaggcttt cccataagcc agcgtcatat aggaagtaat atggatgccg tgctgtttag
     7681 cccaggcaac cactttacgg ttttgcagat aaggagagag ttcaatctgg ttagtagcga
     7741 tgttttcagc gccaataaca gcaatcgcct tttccatcaa tgggatcgtg aagttggaaa
     7801 taccgatctc acgcgtcagc ccttgttttt tggcttccag cagcgcctgc ataaactctt
     7861 caacagagac ttcatcgttt ggtgacggcc agtggattag cgtcagatca acataatcgg
     7921 tacgcaattt ttgcaggctc tctttcagac ttgggatcaa tttgtctttg ctgagatttt
     7981 caatccagat tttagtggtg atgtagagtt catgacgtgg cacgccactt tctgcaatcg
     8041 cctgacctac tgcggcttcg ttatcataga tttgtgcggt atcaattgcg cgataaccaa
     8101 gttcaagcgc cgttttcaca gatgaaataa caacgtcgtc tttcagacgg aaagtaccta
     8161 aaccaaatgc agggatagcc ataatatgcc tcttttaatg ctattttcgt taactgaggt
     8221 gattatgggc ttcagactgt tgaggaaaaa gagcgtaaaa tgcagaggat ttttgcgatt
     8281 ctggcaataa tagatatcca gacgaaaaaa aacctcgagc gttaactcga ggcttcttaa
     8341 taagtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct
     8401 aaccgactga actaccgctc caccgaattc ttttacaacc accggtttta tgaccggctt
     8461 actgcttaat ttgatgcctg gcagtttatg aatcacttcg tgattcaccc ttcgggccgt
     8521 tgcttcgcaa cgttcaaaag ctttcgcttt tgtcctactc tcttatcgag tagtgaactg
     8581 cctcaaagat cagtgccatt cttcg
//
LOCUS       DAZFKM010000183         8407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFKM010000183 DAZFKM010000000
VERSION     DAZFKM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..1598
                     /locus_tag="ACHKAY_004641"
                     /pseudo
     CDS             23..1598
                     /locus_tag="ACHKAY_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1567..3303)
                     /gene="aceK"
                     /locus_tag="ACHKAY_004642"
     CDS             complement(1567..3303)
                     /gene="aceK"
                     /locus_tag="ACHKAY_004642"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO5703179.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3584..4888)
                     /gene="aceA"
                     /locus_tag="ACHKAY_004643"
     CDS             complement(3584..4888)
                     /gene="aceA"
                     /locus_tag="ACHKAY_004643"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO5703180.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4918..6519)
                     /gene="aceB"
                     /locus_tag="ACHKAY_004644"
     CDS             complement(4918..6519)
                     /gene="aceB"
                     /locus_tag="ACHKAY_004644"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO5703181.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6788..7717)
                     /gene="metA"
                     /locus_tag="ACHKAY_004645"
     CDS             complement(6788..7717)
                     /gene="metA"
                     /locus_tag="ACHKAY_004645"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO5703182.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7874..8317
                     /locus_tag="ACHKAY_004646"
     CDS             7874..8317
                     /locus_tag="ACHKAY_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO5703183.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2176 a         2078 c         2062 g         2091 t
ORIGIN      
        1 caataaacaa tgagatattt aaatgattac tcgtatccct cgtagttctt tctctgcaaa
       61 tattaataat acagcccaga caaatgaaca ccaaaccctg agtgaattgt tttataaaga
      121 actcgaggat aaattttctg gcaaggagct ggcgacgcct ctattaaaaa gcttctcaga
      181 gaactgtaga caaaatggtc gtcatatctt tagcaacaag gattttgtca ttaaattttc
      241 cacgtccgtc ttacaagctg ataagaaaga aattacgata attaataaaa acgaaaacac
      301 gacacttact caaaccattg ccccaatatt tgaagaatac ctaatggaaa ttttacctca
      361 acgctcagac actcttgata aacaagaatt aaacctaaaa tcagatagaa aagaaaaaga
      421 attcccaaga attaaactta atggtcaatg ttattttccg gggcgacccc aaaaccgtat
      481 agtatgccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
      541 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
      601 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgtg ttgctgcctg
      661 tggtgatttc gggctagtta tgaaggcata tttcgacaag atggaatcaa atggcatcag
      721 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
      781 gaacacaact gaaggatgta cccattacgt ggtttcggtt tatgatccta atgtcactaa
      841 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
      901 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatatta ttaaccaatc
      961 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gatctgtgga
     1021 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
     1081 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
     1141 aatgttgact gcctataata gtggacatat gaacgttatt aatactattt ttaacgcata
     1201 acccactctg tttaatacgt ttaaattcga taaaaaaaaa tatgaagccc ctcctcctgg
     1261 caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa caacaaaatg
     1321 ttgtagagac ggtttatctt gctttatcta accatgcacg cctgtttgga tttaccgctg
     1381 aagatattat ggatttttgg caacacaaag ccccacaaaa atactctgcc tttgagttgg
     1441 cttttgaatt gggtcaccgg gttattgctg aattaatcct taatacatta aataagatgg
     1501 ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat tatatggaag
     1561 ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc gccgatacgc
     1621 ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc agtaatcagc
     1681 gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt cggcgcatag
     1741 ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc acggttcgct
     1801 ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca cttccgtcat
     1861 gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga caccgaagtt
     1921 tttaaacagc atgtcgccag gaaaaatgtt agcagcggca agctggcgaa tagcgttacc
     1981 gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc agatattgag
     2041 cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac caagatcggt
     2101 gattttttcc gctgcttcct ggagcagtaa ttccattaat gccggggaaa tatgccgctt
     2161 ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac ggtcgtgctc
     2221 tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct gcggcgcgaa
     2281 cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaata ccaacatcac
     2341 cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta gataaacgag
     2401 atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca tatacaattc
     2461 agcagtggtt ttacccggca gaatttcccg tagccactcg accagcgctg cgggcagcgg
     2521 tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt cggcggtcgt
     2581 cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg gcagtaagaa
     2641 gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caagcggctt tattgcggta
     2701 aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc ccagcgtttc
     2761 cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc gcaggcgcag
     2821 cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg ggtgaaagtc
     2881 tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc taaagataaa
     2941 aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg agttaaaaaa
     3001 gctctccgca atctcgaagt gcgggtaatc cggcaacagc cgcgtgtaat gctctttaac
     3061 gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca gttgctccac
     3121 gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct gctggacagc
     3181 atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga ggaatcgacc
     3241 atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca ggccacgcgg
     3301 catttagcgc cctcatcagg agcagagaat tgccggatgc ggcgtaaacg ccttatccag
     3361 cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat caggcatcgt
     3421 gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacgttcggc aacggctgta
     3481 ggcatgataa gacgcgcaag cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg
     3541 tgaacgcctt atccggccta cagtcagcaa cggttgttgt tgcttagaac tgcgattctt
     3601 cagtggagcc ggtcagcgcg gtgactgaag acgtgccgcc ctgaataata gtcgtcactt
     3661 tatcgaagta acctgtaccc acttcctgct ggtgagatac gaaggtatag ccatctttcg
     3721 cggcggcaaa ttccggctgc tgcactttct caacgtagtg cttcataccc tcgccctggg
     3781 cataggcgtt tgccaggtca aacatgttga accacatgct gtggatacct gccagggtga
     3841 tgaactggaa cttgtagccc atatccgaca gctgctgctg gaagctggca atagttttgt
     3901 cgtcgaggtt tttctgccag ttgaacgacg gcgagcagtt ataagccagc agtttgcccg
     3961 gatatttcgc gtggatagct tgtgcaaagc gacgcgccag ttccagatcc ggcgtggagg
     4021 tttcacacca gaccaggtca gcatatggcg catacgccag gccacggctg atcgcttgct
     4081 caatgcccgc atgagtacgg aagaagcctt cactggtacg ctcgccagta ataaattcac
     4141 tgtcatacgg gtcgcaatcg gaggtaatca gatccgccgc atcggcatcg gtacgagcaa
     4201 ccagcagggt tggaacgccc gtcacgtcag ctgcaagacg cgccgcgacc agtttctgaa
     4261 tagcttcctg agttggcacc aaaactttgc cgcccatgtg accgcatttc ttcactgacg
     4321 ccagctgatc ttcgaagtga actgccgctg caccggcttc aatcatcgct ttcatcagtt
     4381 caaaggcatt caggacaccg ccaaaaccgg cttccgcatc ggcaacgatc ggcaggaagt
     4441 aatcgacata gcgcggatcg cccggctcaa tgcctgcgga ccattggatc tgatcggcac
     4501 gacggaaggt gttgttgatc cgctccacca cagctggcac cgagtttgcc ggatagagcg
     4561 actgatccgg atacatgctg gccgccaggt tagcgtccgc cgctacctgc catcccgaca
     4621 gatagactgc ttcaataccc gctttcgcct gttgcagcgc ctgaccgcca gtcagtgcac
     4681 cgaggctgtt gatgtagcct tttttcgact cgccatgcag cagacgccac attttcgccg
     4741 cgcccagttg cgccagggtg cattcaggat tgactgaacc gcgtaatttc accacatctt
     4801 ccgcgctgta tgggcgagta atgccttccc aacgcggttg agtccactct ttctgtaatt
     4861 cttcaatttg ttgtgtacgg gttttcatgt gcagatgctc catagttatg tggtggttta
     4921 cgctaacagg cggtagcctg gcagggtcag gaaatcaatt aactcatcgg aagtggtgat
     4981 ctgttccatc aagcgtgcgg catcgtcaaa acgcccctgg gagaaacgtt cttcgcccag
     5041 ttcgctggca atgactttca tctcttcacc cagcatctgg cggaacaagg ctttggtcac
     5101 cggtttgcca ttgctcaacg ttttttgatg atggatccac tgccagattg aggtacggga
     5161 aatttcagcc gtcgccgcat cttccatcag gccataaatc ggcacgcagc cgttgccgga
     5221 gatccatgct tcgatgtact gcactgccac gcgaatgttg gcgcgcatac cttcttcggt
     5281 gcgttcacca tcacaaggtg ccagcagctg atcggcagta atcggcgcgt cttgttcgcg
     5341 catcacttca agctgatttt tacgggagcc gagaatgtcg ttgaataccg ccattgccgt
     5401 atccgcaagg cctgggtgag caatccatgt gccatcgtga ccgttattgg cttccagcgc
     5461 tttatccgct tttactttgt tgagcacctg gttattgcgc tcttcatctt tgctcggaat
     5521 aaacgctgcc atgccgccca tcgcaaaagc accgcgttta tggcaggttt taatcaacag
     5581 gcgtgagtaa gcattcagga atggtttatc catcgtcact gcctgtctgt ctggcaggac
     5641 gcgatcggga tagtttttca acgttttgat atagctgaag atgtaatccc aacgaccgca
     5701 gttcagacca acaatatggt cacgcagcgc gtgaaggatt tcatccatct ggaacacggc
     5761 gggcagcgtt tcaatcagca acgtcgcctt gatggtgccg cgcggcagat taaagcgatc
     5821 ttctgcatag ctgaagactt cgctccacca ggccgcttcc tgccaggact gggttttcgg
     5881 cagatagaaa tagggaccgc tgccctttgc caacagtgcc tgatagttgt ggaagaaata
     5941 gagcgcaaaa tcaaacaggc taccggggat tgcctcacca cgccaggtga catgtttttc
     6001 cggcaagtgc agaccgcgta cccgacaaat caaaaccgct ggattgggct tgagctgata
     6061 aattttgcct gcttcattgg tatagctgat ggtgccgtta accgcatcgc gcaggttaat
     6121 ttgcccgtcg atcactttgt tccagtctgg tgccagtgaa tcttcgaaat cggccataaa
     6181 gactttcaca ttggcgttca gtgcgttgat caccatcttg cgctcaaccg ggccagttat
     6241 ctcgactcga cgatcttgta agtccgcggg aataccacga attttccagt ccgtatcgcg
     6301 aatggaagct gtttccgaaa taaaatcagg caaagttccg ttatcaatat cttgctgctg
     6361 ctgaatgcgc gctgccagaa gtttattgcg ttgtggcgta aaatgcgtca ccagctcagt
     6421 cagaaattct accgcttcgg cagtaagaat ttgcttctcc tgctcgccat acggccttat
     6481 gaaagccagt tcatcggttg ttgttgcctg ttcagtcatc gtgcagctcc tcgtcatgga
     6541 cccgaaaact tccccactgt gaacgaagga tcgttgtgcg cttttcgttc agcacaacta
     6601 agactactca tttaaaatgc aaaatcaaaa acaatttcca ttttaattaa aaatacttga
     6661 taacttattg ataacaatat gaataaaatt tattcacctc ctgaggtgcg tttcggaaat
     6721 aaaaaaggca cccgaaggtg cctgaggtaa ggtgctgaat cgcttaacga tcgactatca
     6781 cagaagatta atccagcgtt ggattcatgt gccgtagatc gtatggcgtg atctgataga
     6841 cgtaatagtt gagccagttg gtaaacagca aattaccgtg actacgccag ctcgctcgcg
     6901 gtgtattttg cggatcattg tgcgggaaat agttatacgg tacttccggg cctagtccgg
     6961 cttccacatc gcggaaatat tcctgcgcca gcgtttgcgc atcatattcg ggatggcccg
     7021 tcacaaaggc aatgcgctta tctttgctgg caaacagata tgcatcccct tcttccgtct
     7081 ctgccagaat ttccagatcg gtgtaatcac gaatcaacgc tgccggaaag tcagcatagc
     7141 gcgaatgtgg tgccaggaat gaatcatcaa agccacgcgt cagaagcgca tgaggatgga
     7201 gaatatgatg ctcgtaaacg ccagagagtt tgtcggtgcg agtttgctta ggaatgccgt
     7261 agaggatatt gagcgcggcc tgtaccgccc agcagacaaa cagcgtcgag gtgacgtgat
     7321 ctttcgacca ctccagcacc tgtttgatct gcggccagta agcgacatca ttaaactcca
     7381 ccaggcccag cggcgcaccg gttacaatca gaccgtcaaa gttctgttcc tgaatatctt
     7441 caaagttaca gtagaagttg ttcagatgct ctgcgggcgt gttgcgcgat tcacgggaat
     7501 cgatgcgcaa cagctgaata tcgacctgca aaggtgagtt tgaaagcagg cgcagaaact
     7561 gattttcagt ttcaatcttc ttcggcatca ggttaaggat tagaacctta agtggacgaa
     7621 tttcctgacc agacgcacga gaagttgtca tcacaaagac gttttcttca cgcaagaaat
     7681 tgacggcggg tagctcgtcc ggcacacgaa taggcataac ctgattacct cactacatac
     7741 gcttatacgt ttagacatcc agatagctga agataaccag aaaatttgcc aatgtcgagc
     7801 ctgcatgttg aaggtgagaa agtttcaagg aaaaggctac gttagaatat aagaatgacg
     7861 aaaaggagag aaaatggtta ttagtattcg ccgctcacag catgaggaag gggaggaact
     7921 cgttgcgatt tggtgtcgat ctgtcgatgc cacccacgat tttttatcag cagagtatcg
     7981 ggccgagctg gaagagctgg ttcgttcctt tctgccggaa gcaccgttgt gggtcgcggt
     8041 taatgaacgg gatcagccgg ttgggtttat gttgttaagt gggcagcata tggatgcgct
     8101 gtttatcgat cctgatgtgc gcggctgcgg cgtgggtcgg atgctggtgg agcatgcgct
     8161 ctcgatggca ccggaactga caaccaacgt taatgagcaa aatgagcagg cggttgggtt
     8221 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
     8281 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
     8341 ggctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcaccaacgc aaaaaggcca
     8401 tcgtgac
//
LOCUS       DAZFKM010000184         8320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFKM010000184 DAZFKM010000000
VERSION     DAZFKM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            86..346
                     /gene="dinJ"
                     /locus_tag="ACHKAY_004647"
     CDS             86..346
                     /gene="dinJ"
                     /locus_tag="ACHKAY_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO5703184.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            334..621
                     /gene="yafQ"
                     /locus_tag="ACHKAY_004648"
     CDS             334..621
                     /gene="yafQ"
                     /locus_tag="ACHKAY_004648"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO5703185.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            777..1517
                     /gene="dpaA"
                     /locus_tag="ACHKAY_004649"
     CDS             777..1517
                     /gene="dpaA"
                     /locus_tag="ACHKAY_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO5703186.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1488..2255)
                     /gene="yafJ"
                     /locus_tag="ACHKAY_004650"
     CDS             complement(1488..2255)
                     /gene="yafJ"
                     /locus_tag="ACHKAY_004650"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO5703187.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2461..3039)
                     /gene="lpcA"
                     /locus_tag="ACHKAY_004651"
     CDS             complement(2461..3039)
                     /gene="lpcA"
                     /locus_tag="ACHKAY_004651"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO5703188.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3279..5723
                     /gene="fadE"
                     /locus_tag="ACHKAY_004652"
     CDS             3279..5723
                     /gene="fadE"
                     /locus_tag="ACHKAY_004652"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO5703189.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5766..6212)
                     /gene="ivy"
                     /locus_tag="ACHKAY_004653"
     CDS             complement(5766..6212)
                     /gene="ivy"
                     /locus_tag="ACHKAY_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO5703190.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6393..7160
                     /gene="yafV"
                     /locus_tag="ACHKAY_004654"
     CDS             6393..7160
                     /gene="yafV"
                     /locus_tag="ACHKAY_004654"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO5703191.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(7384..>8320)
                     /locus_tag="ACHKAY_004655"
     CDS             complement(7384..>8320)
                     /locus_tag="ACHKAY_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO5703192.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         1996 a         2073 c         2144 g         2107 t
ORIGIN      
        1 ttattaaatt actcatggat tgtcgctgtt gctcatttga gctacaattc aagctgaata
       61 aatatacagc acaggagata ccccaatggc tgctaacgca tttgttcgcg cccgaatcga
      121 tgaagatctg aagaatcagg cggcggacgt actggccggg atggggctga ccatctctga
      181 cctggttcgc ataaccctca caaaggtcgc gcgtgaaaag gcattgccgt ttgatttacg
      241 cgagcctaat caattaacca ttcaatcaat caaaaacagc gaagctggcg ttgatgttca
      301 taaggccaaa gacgccgatg atttatttga taaattagga atttaaatca aagggatatt
      361 gaatactcgg gacaattttc aaaggatgta aaacttgcac aaaagcgtca taaggatatg
      421 aataaattga aatatcttat gacgcttctt atcaataatg ctttaccgct tccagctgtt
      481 tataaagacc acccgctgca aggttcatgg aaaggttatc gcgatgctca tgtcgaaccg
      541 gactggatcc tgatttacaa acttaccgat aaacttttac gatttgagag aactggaact
      601 cacgcggcgc tctttgggta aagcacgctg ccgatgaaat ttgtaaaccc tgcgccgaca
      661 ggcttgcgta aaactctttt ggtgataatg tgagccttcc aaatccagac tattccgatt
      721 tgggatctat tttgccgtcc tggcgtgtgt aaccgtttta tcaaggaata agcagtatgc
      781 gtaaaatcgc attaattctt gcgatgttgt tgatcccgtg cgtttcgttt gccggtttgc
      841 tgggcagcag tagctcgacc acgccagtga gcaaagagta taagcagcag ttgatgggat
      901 cccctgtcta catccagatc ttcaaggaag aacgtacgct cgatctctac gtcaaaatgg
      961 gcgagcaata tcagctgctc gacagttata aaatctgtaa atattccggc ggcttagggc
     1021 caaaacagcg tcagggcgat ttcaaaagcc cggaagggtt ttatagcgtc cagcgtaatc
     1081 agttaaaacc agacagccgt tactacaaag cgattaatat tggtttcccc aatgcctatg
     1141 accgtgcgca tggttatgaa gggaaatacc tgatgatcca cggcgattgt gtttccatcg
     1201 gctgctacgc aatgaccaat cagggtattg atgagatctt ccagttcgtt actggtgcgc
     1261 tggtgtttgg tcagccgagc gtgcaagtga gtatttaccc gttccgcatg accgacgcca
     1321 atatgaagcg ccataaatat tccaacttta aggacttctg ggagcagctg aagccgggct
     1381 acgactactt tgagcaaacc cgtaagccac cgaccgtttc tgtggtcaat ggccgttacg
     1441 tggtcagcaa gccgttaagc cacgaagttg tgcagccaca actggcatca aactacacgc
     1501 tccccgaggc aaaataagcg ccattcgcct ggcataatct tttgccaggt ttcattgccc
     1561 gtcagcggct gtgttgcaat caccgtgacc acatcattcg gtgtggtctg cgagctgaag
     1621 tcgatttcca catcctgatc cagcagcgtt gccacgccaa acggcgcgcg gcgggtgatc
     1681 cagtgtaaat tagtcgagca atacgccatc acatagcgcc cgtcagaaag cagcatgttg
     1741 aaaacgccct tctgccgcag ttcatccgcc agtgaggcga tatatttaaa cactgccgcc
     1801 atgttgcccg gtgtgcgcgg gtaacgctgc gttaatttat gcaggagcca gcagaaggct
     1861 ttttcgctat cggtttcacc gaccgggcgg aagttgccgg tttccagtga tttgtagccc
     1921 gtcagttgtc cgttatgggc ataagtccag ttacgccccc ataactcgcg ggtaaatggg
     1981 tgagtatttt ccagcgccac ctcgccccga ttagcctggc gaatatgagc caccaccgaa
     2041 caggatttta tcgggtagtc ctggacaagt ttggcgatgg gggaattaaa gctgggttgt
     2101 ggatctttaa atgtgcgaca gcctttacct tcgtaaaagg taatgcccca gccatcttta
     2161 tgtggcccgg ttccaccacc acgctgtaca agtccggtga aactaaagca gatatcggta
     2221 gggacgttgg cgctcatccc gagcaattcg cacatgggta acctccacaa cagcgggagc
     2281 gaatggcccc cggcgaagat tgatttaaga attttcaggt cggatgcggc gtaaacgaat
     2341 tattcggcct acggttcggg tacagatttg taggcatgat aagacgcgtc agcatcgcat
     2401 caggcatcag cgcacaagtg ccggatgcgg cgtaaacgcc ttatccagcc tacgccagac
     2461 ttacttaacc atctcttttt caattaactg gatcaggata tggatcactt taatgtgaat
     2521 ctcctgaatg cggtcggcat aaccaaagtg cggtacgcga atttcgatat ccgccgtgcc
     2581 agccattttg ccgccgtctt taccggtcag ggtgatcact ttcatcccct tctcacgcgc
     2641 cgctgcgatc gctttgatca cgtttgcaga gttaccggag gtggagatcc ccagcagtac
     2701 atcgccttcg cgacctaccg cttcaacgta gcgggagaaa atatcattga aaccgaaatc
     2761 attaccgacg caggaaatat gactaacgtc agaaatagca atcgccgggt agcccggacg
     2821 gttttcacgg tagcgaccgg tcaactcttc ggcaaagtgc atagcgtcgc aatgggaacc
     2881 gccgttgccg caggaaagca ctttgccacc ggctttaaag ctgtctgcta acaggaccgc
     2941 cgcgcgctga atggcgtgaa tattggcgtc atcttttaaa aagttagcca gcgtttccgc
     3001 cgcttcgttc agttcgttac gaataagatc ctggtacatg aggatatcct tcagcataaa
     3061 tgtaatagac aaaatgcagt gtaccggata ccgccaaaag cgagaagtac gggcaggtgc
     3121 tatgaccagg actttttgac ctgaagtgcg gataaaaaca gcaacaatgt gagctttgtt
     3181 gtaattatat tgtaaacaca ttgctaaatg tttttacatc cactacaacc atatcatcac
     3241 aagtggtcag acctcctaca agtaaggggc ttttcgttat gatgattttg agtattctcg
     3301 ctacggttgt cctgctcggc gcgttgttct atcaccgcgt gagcttattt atcagcagtc
     3361 tgattttgct cgcctggaca gcctccctcg gcgttgctgg tctgtggtcg gcgtgggtac
     3421 tggtgcctct ggccattatc ctcgtgccat ttaactttgc gcctatgcgt aagtcgatga
     3481 tttccgcgcc ggtatttcgc ggtttccgta aggtgatgcc gccgatgtcg cgcactgaga
     3541 aagaagcgat tgatgcgggc accacctggt gggagggcga cttgttccag ggcaagccgg
     3601 actggaaaaa gctgcataac tatccgcagc cgcgcctgac cgccgaagag caagcgtttc
     3661 tcgacggccc ggtagaagaa gcctgccgga tggcgaatga tttccagatc acccatgagc
     3721 tggcggatct gccgccggag ttgtgggcgt accttaaaga gcatcgtttc ttcgcgatga
     3781 tcatcaaaaa agagtacggc gggctggagt tctcggctta tgcccagtct cgcgtgctgc
     3841 aaaaactctc cggcgtgagc gggatcctgg cgattaccgt cggcgtgcca aactcattag
     3901 gcccgggcga actgttgcaa cattacggca ctgacgagca gaaagatcac tatctgccgc
     3961 gtctggcgcg tggtcaggag atcccctgct ttgcactgac cagcccggaa gcgggttccg
     4021 atgcgggcgc gattccggac accgggattg tctgcatggg cgaatggcag ggccagcagg
     4081 tgctggggat gcgtctgacc tggaacaaac gctacattac gctggcaccg attgcgactg
     4141 tgcttgggct ggcgtttaaa ctctccgacc cggaaaagct gctgggtggc gcagaagatt
     4201 taggtattac ctgtgcgctg atcccgacca ccacgccggg cgtggaaatt ggtcgtcgcc
     4261 acttcccgct gaacgtaccg ttccagaacg gaccgacgcg cggtaaagat gtcttcgtac
     4321 cgatcgatta catcatcggt gggccgaaaa tggccgggca aggctggcgg atgctggtgg
     4381 agtgcctctc ggtaggccgc ggcatcaccc tgccttccaa ctcaaccggc ggcgtgaaat
     4441 cggtagcgct ggcaacgggc gcgtatgctc acattcgccg tcagttcaaa atctctatcg
     4501 gtaagatgga agggattgaa gagccgctgg cgcgtattgc cggtaatgcc tacgtgatgg
     4561 atgctgcggc atcgctgatt acctacggca ttatgctcgg cgaaaaacct gccgtgctgt
     4621 cggctatcgt taagtatcac tgtacccacc gcgggcagca gtcgattatt gatgcgatgg
     4681 atatcactgg tggcaaaggc attatgctcg ggcagagcaa cttcctggcg cgtgcttacc
     4741 agggcgcacc gattgccatc accgttgaag gggccaacat tctgacccgc agcatgatga
     4801 tcttcggaca aggagcgatt cgttgccatc cgtacgtgct ggaagagatg gaagcagcga
     4861 agaacaatga cgtcaacgcg ttcgataaac tgttgttcaa acatatcggt cacgtcggta
     4921 gcaacaaagt tcgcagcttc tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa
     4981 ccggcgatgc cactaaacgc tactatcagc acctgaaccg cctgagcgcc aacctcgccc
     5041 tgctttctga tgtctcgatg gctgtgctgg gcggcagcct gaaacgtcgt gagcgcatct
     5101 cggcacgtct gggggatatt ttaagccagc tttacctcgc ctctgccgtt ctgaagcgtt
     5161 atgacgacga aggccgtaat gaagccgacc tgccgctggt gcactggggc gtacaagatg
     5221 cgctgtatca ggctgaacag gcgatggatg atttactgca aaacttcccg aaccgcgtgg
     5281 ttgccgggct gctgaatgtg gtgatcttcc cgaccggacg tcattatctg gcaccttctg
     5341 acaagctgga tcataaagtg gcgaagattt tacaagtgcc gaacgccacc cgttcccgca
     5401 ttggtcgcgg tcagtacctg acgccgagcg agcataatcc ggttggcttg ctggaagagg
     5461 cgctggtgga tgtgattgcc gccgacccaa ttcatcagcg gatctgtaaa gagctgggta
     5521 aaaacctgcc gtttacccgt ctggatgaac tggcgcacaa cgcgctggcg aaggggctga
     5581 ttgataaaga tgaagccgct attctggtga aagctgaaga aagccgtctg cgcagtatta
     5641 acgttgatga ctttgatccg gaagagctgg cgacgaagcc ggtaaagttg ccggagaaag
     5701 tgcggaaagt tgaagccgcg taattgaata acggagccga aaggctccgt ttctttatcc
     5761 gctaattatt taaaattaaa gccatccgga tggttttcca ggctgccggt caacgccgcg
     5821 aacagcaccg ttttaccatc aatcgaaagc gcatcgttca cattcagcca ggtgagtttc
     5881 tcttgcgacg ttttctcatc aatagtcgag aacagccccg tcatctgatt agatttctcg
     5941 gaccacatca cagcgatacg ttgcgagcca cagtcatgcg gtttgcacgc gctcatcacc
     6001 tgatacgtct catctcccaa cgttacggtt tgtgcgggag tataagtacc gcctttcatc
     6061 acccaggcag gcagcttatg cccttgtacc atctgattaa atgcagcttt ggtggtttcg
     6121 ccctttgcaa ggctgctaat ggttaaatca tcctgcgcca ttgcactggt ggcgatgacc
     6181 agagcggcga ctgtcgttat tgccttaaac atcattcctc ccgagcttat cctgcccatg
     6241 ttattaacct tctaaagcct ggcgataaca cgataatttg caaatcagat atttccaata
     6301 tcagcatttg ttatcaattt attctgcgct aaggcaactg cctgataagg tgttaaagtt
     6361 ataaaaccct tcgcataaag gagttgtaca ccgtgcctgg tttgaagatt acgcttttgc
     6421 agcaaccact ggtgtggatg gatggccctg ccaacctgcg tcattttgat cgtcaactgg
     6481 aaggtattac cgggcgcgat gtgatcgttc taccggagat gtttaccagc ggctttgcca
     6541 tggaagcggc agcttcgtcg ctagcacaag atgacgtagt gaactggatg acagctaagg
     6601 cgcagcagtg caatgcgctg attgcaggca gtgttgcatt acaaacggag tctggttcgg
     6661 ttaaccgctt tttgctggtt gagccgggcg gcacggtaca tttttatgat aagcgtcatc
     6721 tgttccgcat ggcagatgag catctacatt ataaagcggg caatgcgcga gtgattgtgg
     6781 aatggcgcgg ctggcgtatt ttgccgctgg tgtgctacga cttacgtttt cctgtgtggt
     6841 cgcgcaatct caacgattat gacctcgccc tgtacgtcgc caactggcct gctccgcgct
     6901 ctctgcactg gcaggcattg ctgacggccc gcgcgattga gaatcaggcg tatgtggcgg
     6961 gatgcaatcg cgtcggcagc gatggcaacg gctgccatta tcgcggtgac agccgggtga
     7021 ttaatccaca aggcgagatt atcgctactg ccgacgcgca tcaggcaacg cgcattgatg
     7081 ccgagctgtc gatggcggtg ttaagggaat atcgcgataa gtttccggca tggcgggatg
     7141 cggatgagtt taggttgtag tgagtggggg ctgtatttag ggcggggaat tcagaaaggg
     7201 atgtttagtg tgctgagcga gagttaaata ataaaaaact gggaagattt aataatcccc
     7261 ccgcagggca agagcatgaa ttaaatttgt gttaaatttt tcattttatc agttcgaaac
     7321 ccaagctctg agcaggatgc aagcacttct tctatataac atgtgtttaa cgccagggca
     7381 agattacgaa agcccgctcc ccgcaaggac tgacgccaga tagtttctgt ccatggctgc
     7441 ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca aaatattaat
     7501 agcaatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta ttttgcagtc
     7561 gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt gccagtgatt
     7621 tcggatcgct gtggcgaatt tctcagcggt taaatcagca gaactgatat aatatctgac
     7681 cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga ctgccacgca
     7741 taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag ggacatcgca
     7801 aacaatatga agacggattt cttctctgcc gtgactcttt tcactaattg cgtaactgtc
     7861 atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt tatttagccg
     7921 cccctggttt ccttttaccg cgaataaata atcacctccc tgtttttgta tcttctctgc
     7981 aatatctttc tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa tatccagcat
     8041 gttaagaagt tctgggatag ctgtaatctc attagatttc tcatccgtcc tgatctgtcc
     8101 gatgaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg
     8161 actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcgatgacgt ctttatcatc
     8221 tgaagaatgg cagtcacgca tccagttaat aaagcactca tgaaattttg caggactgat
     8281 acaggataca actctggcaa tagtatcgtg aacaggaata
//
LOCUS       DAZFKM010000185         8190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFKM010000185 DAZFKM010000000
VERSION     DAZFKM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            95..925
                     /locus_tag="ACHKAY_004656"
     CDS             95..925
                     /locus_tag="ACHKAY_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO5703193.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            957..1892
                     /locus_tag="ACHKAY_004657"
     CDS             957..1892
                     /locus_tag="ACHKAY_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO5703194.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1985..2227
                     /gene="yhdT"
                     /locus_tag="ACHKAY_004658"
     CDS             1985..2227
                     /gene="yhdT"
                     /locus_tag="ACHKAY_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO5703195.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2217..3668
                     /gene="panF"
                     /locus_tag="ACHKAY_004659"
     CDS             2217..3668
                     /gene="panF"
                     /locus_tag="ACHKAY_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO5703196.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3680..4561
                     /gene="prmA"
                     /locus_tag="ACHKAY_004660"
     CDS             3680..4561
                     /gene="prmA"
                     /locus_tag="ACHKAY_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO5703197.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4890..5855
                     /gene="dusB"
                     /locus_tag="ACHKAY_004661"
     CDS             4890..5855
                     /gene="dusB"
                     /locus_tag="ACHKAY_004661"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO5703198.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5881..6177
                     /gene="fis"
                     /locus_tag="ACHKAY_004662"
     CDS             5881..6177
                     /gene="fis"
                     /locus_tag="ACHKAY_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO5703199.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6263..7147
                     /gene="yhdJ"
                     /locus_tag="ACHKAY_004663"
     CDS             6263..7147
                     /gene="yhdJ"
                     /locus_tag="ACHKAY_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO5703200.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7231..7410
                     /gene="yhdU"
                     /locus_tag="ACHKAY_004664"
     CDS             7231..7410
                     /gene="yhdU"
                     /locus_tag="ACHKAY_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO5703201.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7413..8075)
                     /gene="acrS"
                     /locus_tag="ACHKAY_004665"
     CDS             complement(7413..8075)
                     /gene="acrS"
                     /locus_tag="ACHKAY_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO5703202.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         2024 a         1975 c         2061 g         2130 t
ORIGIN      
        1 tggcccatga ttttcttttc gaattgtcga tattattgaa ggataacttt caaagaaaaa
       61 tacaaggggc tatagtgtta cgcattaaag gccaatgaag agtgttaacg tgccgagaaa
      121 atcgtctaac ctactgaaac gcagattaaa aattgcagaa attgttgccg cccaaggtga
      181 aatcaaagtt gatgacttat ctgcgcaact tggtgtctct ggcgtgacga tccgcggcga
      241 tctcagttat ctggagcaac agggatattt aaagcgatca tttggcggtg ctattgcgac
      301 accactctcc tctgaaccag aagcgataca accgatcgcg ccattgtctc tggcacaaca
      361 aatggaaata gcacgacatt gcgcacggtt aattcgcgat cgtgacacgc tatttcttgg
      421 acatgggaca atctgccgta agatcattcc attgctcagc gaagtgaaaa aactgcgtct
      481 gatcaccaac gatcccgaac atgcgctgct ggcaaatcag tttattgatg gcgaaataat
      541 actggctggc agtgaaatgc tgcgccccga caccgcactg gccggaaaac cgcttgaaca
      601 ggcacttcag catttcacca ttacacatag cattctggaa atcagccatc tggatgctga
      661 tggtacttta aatatcaacg ttcccagact ggccgctgcc tggcagctct gtttcgatca
      721 tgcccagaat aagacggtta tcgttgccgc cgatcctgcg ccatcaacgg ctgctatcgg
      781 gcatttgcat caggcagaaa atgtgatcgt cagccgcagc attaacgaac aatatcagca
      841 acaattgcag tcggctgatt tcgttatcct ttataccagt aatgagtgct ttacctggtc
      901 caatcgcgaa aggcttcagg aatgagaggg gttttgtgac aataactatc tgcgctatgg
      961 gcgaattgct ggccgagttt ttgtcccgca acccacatca aaaattcact cagcctgggg
     1021 agtttatcgg gccatttccc agcggtgcgc cagcaatttt tgctgctcag gtggcaaaac
     1081 tgtcccatcg ggccatcttc ttcggatgtg ttggtaatga tgattttggc cgactcatta
     1141 tagagcgcct ccgtcatgaa ggtgtcatta ccgatgggat ccatgttatg aacaatgccg
     1201 tcacaggtac ggcgttcgtg agttatcaaa atccccagca gcgcgatttc gtctttaata
     1261 tccctaacag cgcctgcggc ttgtttactg ctgagcacat tgataaggat ctgctaaaac
     1321 agtgtaacca tctccatatt gtgggctcat cgttgttctc atttcgcatg atcgatgtca
     1381 tgcgtaaagc aataacgacg atcaaatcgg ctggcggcac cgtttctttc gatcccaata
     1441 ttcgcaaaga gatgctgagc attcctgaaa tggcgcaggc tctcgattat ttgattgaat
     1501 atacggatat tttcatcccc agcgaaagcg aactcccttt cttcgcgcgt cacaaaaatc
     1561 tgtcagaaga acagattgtt agcgatcttc tccacggcgg cgtaaaacat gtggcgataa
     1621 aacgcgccca gcgtggggcc agctattaca agcttaaaaa cggtacatta cacgcccagc
     1681 atgttacagg ccacgatatc gaaattatcg atccaacggg tgcaggcgac tgctttggcg
     1741 caacgtttat cactcttttc ttatccggtt tcccggcaca taaggcactg caatatgcaa
     1801 atgccagcgg cgcgctcgcc gtaatgcggc aaggtccgat ggaagggata tcctcactgg
     1861 ctgacattga agactttttg cagcagcact gaacggcaat tttctgtcag attattttcc
     1921 ctgcctgaag agttgccgta gaattgcccg tttctcgtat ccccccatga ggaggaaccc
     1981 ttccgtggac actcgttttg ttcaggccca taaagaggcg cgctgggcgc tggggctgac
     2041 ccttttgtat ctggcagttt ggttagtagc cgcttactta cctggcgttg cccccggttt
     2101 taccggcttt ccgcgctggt ttgagatggc ctgcatcctg acgccgctgc tgtttattgg
     2161 actgtgctgg gcgatggtga aatttatcta tcgcgatatc ccactggagg atgacgatgc
     2221 agcttgaagt aattctaccg ctggtcgcct atctggtggt ggtgttcggt atctcggttt
     2281 atgcgatgcg taaacggagc accggcacct tccttaatga gtatttcctc ggcagccgct
     2341 ctatgggcgg tattgtgctg gcgatgacgc tcaccgcgac ctatatcagt gccagttcgt
     2401 ttatcggcgg gccaggagct gcttataaat acgggctggg ctgggtattg ctggcgatga
     2461 ttcagcttcc tgcagtctgg ctttcactcg gtattctcgg caagaagttt gcgattcttg
     2521 cgcgccgcta caatgcagtg acgctgaacg atatgctgtt tgcccgctac cagagtcgtc
     2581 ttctggtgtg gctggcgagt ttgagtttgc tggttgcgtt cgttggtgcg atgaccgtgc
     2641 agtttatcgg cggtgcgcgc ctgctggaaa ccgcggcggg tattccttat gaaaccgggc
     2701 tgctgatttt tggtatcagc attgcgttat ataccgcctt tggtggcttt cgcgccagcg
     2761 tgctgaacga caccatgcaa gggcttgtga tgctgattgg caccgttgtg ctgcttattg
     2821 gcgtggtaca tgccgctggc ggcttaagca acgcagtaca gaccttgcaa accatcgatc
     2881 cgcaactggt tacgccacaa ggcgctgacg atattctgtc gcctgccttt atgacgtcgt
     2941 tctgggtact ggtgtgcttt ggcgtgattg gcctgccgca tactgcggtg cgctgtatct
     3001 cttataaaga cagcaaagca gtacaccggg ggatcatcat cggtacgatt gtagtcgcaa
     3061 tcctgatgtt cggtatgcac ctggccggag cgttaggtcg tgcagtgatc cccgatctca
     3121 ccgtaccgga tctggtgatc ccgacgttaa tggtaaaagt gctgccaccg tttgctgccg
     3181 gaatcttcct ggctgcacct atggctgcga tcatgtcgac gatcaatgcc cagttgctgc
     3241 aaagttccgc tacgatcatt aaagatctct atctgaatat ccgtccggat caaatgcaaa
     3301 acgagacgcg tctgaagcgg atgtcggcgg taattacgtt agttctcggc gcgttgctgc
     3361 tgcttgccgc ctggaagccg ccagaaatga tcatctggct gaatttgctg gccttcggtg
     3421 ggctggaagc cgttttcctg tggccgctgg tgctgggtct ttactgggaa cgcgccaacg
     3481 ccaaaggcgc gctaagtgcg atgatcgttg gcggcgtgct gtatgccgta ctcgcgacgc
     3541 tgaatattca gtacctgggc ttccacccta ttgtgccctc gttactacta agtttgctgg
     3601 ctttcctggt cggaaaccgt ttcggtacat ccgtcccgca agctaccgtt ttgactactg
     3661 ataaataaag agttttgcca tgccttggat ccaactgaaa ctgaacacca ccggcgcgaa
     3721 cgcggaagat cttagcgatg cgctgatgga agcgggtgcc gtttctatca cttttcagga
     3781 tacccacgat acgccagtat ttgagccgct gccgggcgaa acgcgcctgt ggggcgacac
     3841 cgatgtgatt ggtctgttcg acgctgaaac cgatatgaac gacgtggtgg cgattctgga
     3901 aaaccatccg ctgctcggcg caggcttcgc gcataaaatc gaacaactag aagataaaga
     3961 ctgggagcgc gaatggatgg ataatttcca cccgatgcgc tttggtgaac gactgtggat
     4021 ctgccctagc tggcgtgatg tgccggacga aaacgccgtc aacgtgatgt tagatccagg
     4081 gctggcgttt ggtacgggta cccatccaac cacctctctg tgcctgcaat ggctcgacag
     4141 cctcgattta accggtaaaa cagtcatcga ctttggctgt ggttccggca ttctggcgat
     4201 cgcggcgctg aaactgggtg cagcaaaagc cattggtatt gatatcgatc cgcaggcgat
     4261 tcaggccagc cgcgacaacg ccgaacgtaa tggcgtttct gaccgtctgg aactctactt
     4321 accgaaagat cagccagaag aaatgaaagc cgacgtggtg gtcgctaaca tccttgcagg
     4381 cccattacgt gaactggcac cgttaatcag cgtcctgccg gtttcaggcg gtttgctggg
     4441 cctttccggt attctggcaa gccaggcaga gagcgtttgt gaagcttatg ccgatagctt
     4501 cgcactggac ccggtcgtgg aaaaagaaga gtggtgccgt attaccggtc gtaagaatta
     4561 accttcgcat cgccgtaggg tgacgcgggg cgaagtgcga gcaagctcac aaaaggcacg
     4621 taaatttgcc ggttatttac gcaaatttgc gtgccaaaat tttcattcat aaagaaaaat
     4681 tgagaactta ctcaaatttc tttgagtgta aattttagtc actattttct aatatgatga
     4741 tttttatgag taattatcgc accacgctca ttttaaatgc aattctttga tccatctcag
     4801 aggattggtc aaagtttggc ctttcatctc gtgcaaaaaa tgcgtaatat acgccgcctt
     4861 gcagtcacag tatggtcatt tcttaactca tgcgcatcgg acaatatcag ctcagaaatc
     4921 gcctgatcgc agcgcccatg gctggcatta cagacagacc ttttcggacg ttgtgctacg
     4981 agatgggagc cggattgaca gtatccgaga tgatgtcttc taacccacag gtttgggaaa
     5041 gcgacaaatc tcgtttacgg atggtgcaca ttgatgaacc cggtattcgc accgtgcaaa
     5101 ttgctggtag cgatccgaaa gaaatggcag atgcagcacg tattaacgtg gaaagcggtg
     5161 cccagattat tgatatcaat atgggttgcc cggctaaaaa agtgaatcgc aagctcgcag
     5221 gttcagccct cttgcagtac ccggatgtcg ttaaatcgat ccttaccgag gtcgtcaatg
     5281 cagtggacgt tcctgttacc ctgaagattc gcaccggctg ggcaccggaa caccgtaact
     5341 gcgaagagat tgcccaactg gctgaagact gtggcattca ggctctgacc attcatggcc
     5401 gtacacgcgc ctgtttgttc aatggagaag ctgagtacga cagtattcgg gcagttaagc
     5461 agaaagtttc cattccggtt atcgcgaatg gcgacattac tgacccgctt aaagccagag
     5521 ctgtgctcga ctatacaggg gcggatgccc tgatgatagg ccgcgcagct cagggaagac
     5581 cctggatctt tcgggaaatc cagcattatc tggacactgg ggagttgctg cccccgctgc
     5641 ctttggcaga ggttaagcgc ttgctttgcg cgcacgttcg ggaactgcat gacttttatg
     5701 gtccggcaaa agggtaccga attgcacgta aacacgtttc ctggtatctc caggaacacg
     5761 ctccaaatga ccagtttcgg cgcacattca acgccattga ggatgccagc gaacagctgg
     5821 aggcgttgga ggcatacttc gaaaattttg cgtaaacaga aataaagagc tgacagaact
     5881 atgttcgaac aacgcgtaaa ttctgacgta ctgaccgttt ctaccgttaa ctctcaggat
     5941 caggtaaccc aaaaacccct gcgtgactcg gttaaacagg cactgaagaa ctattttgct
     6001 caactgaatg gtcaggatgt gaatgacctc tatgagctgg tactggctga agtagaacag
     6061 cccctgttgg acatggtgat gcaatacacc cgtggtaacc agacccgtgc tgcgctgatg
     6121 atgggcatca accgtggtac gctgcgtaaa aaattgaaaa aatacggcat gaactaattc
     6181 aggttagcta aatgcttgat taaaaaggcg ctactcggca tggggaagcg ccttttttat
     6241 aggtgtcaca aagggagtga ccatgagaac aggatgtgaa ccgacccggt ttggtaatga
     6301 agctaagacc attattcacg gtgatgcgct tgccgaactt aaaaagatcc ccgccgaaag
     6361 tgtcgatctg atctttgccg acccaccata taacatcggt aaaaattttg atggtctgat
     6421 cgaagcctgg aaagaagatc tgtttatcga ctggctgttt gaagtgattg cagagtgcca
     6481 ccgcgttctg aaaaagcagg gcagcatgta catcatgaac agcacggaaa acatgccctt
     6541 tatcgatctc cagtgccgca agctttttac catcaaaagt cgcatcgtct ggtcatatga
     6601 cagttctgga gtacaggcga aaaaacacta cggctccatg tacgaaccca tcctgatgat
     6661 ggtgaaagac gcaaagaact acacattcaa cggtgatgct attctggtag aagccaaaac
     6721 cggatcgcag cgcgcgttga tcgattatcg caaaaatcct ccacagccat acaatcatca
     6781 aaaagtaccg ggtaacgtct gggattttcc gcgcgtgcgt tatttgatgg atgaatatga
     6841 gaaccacccg acgcaaaaac cggaagcctt actgaaacgc attattctcg cctcttccaa
     6901 cccaggcgat atcgttctcg acccgtttgc cggtagcttt actaccggtg ccgtagccgt
     6961 ctccagcggg cgaaaattca ttggtattga gatcaacagc gagtacatca aaatggggct
     7021 tcgacggctg gatgtcgcgt cgcattactc tgcggaagaa ctggcgaaag tgaaaaaaag
     7081 aaagaccggc aacctgtcaa aacgaagccg gttaagcgaa gttgaccccg atctcattgc
     7141 aaagtaaagg aagtgtaagc ctggttttca gattattcat ttcgtgtata tttcctgcca
     7201 gacttggtta aacatacaca ggctcaggta atgattcgca agtattggtg gctcgtcgtt
     7261 ttcgctgtct tcgtttttct gtttgatact ttactgatgc agtggattga actgctggca
     7321 acagaaacag acaaatgccg caatatgaac tcagttaatc cactaaaact ggttaactgt
     7381 gacgaactga attttcagga cagaatgtga atttacatga cacttaattc attcgtttga
     7441 tgaattaatt tcgttatgtt ttcatctggc atgaacattc ttaatacgtt atcgaccaga
     7501 gcgggggctt gtttataaag atcataaccc gccatattca ttaaccagtt ttgaacaatt
     7561 ccgctgaagg caccattaat aataatcatc acaacatcta aatcgaggtt atttgctaca
     7621 caaccttgtt gctgacacgc ctgcaatact tcgcggagag tctgcggatt gaagcccatc
     7681 ttttcgcgta tcactccctc ggccagcatc tcatcattaa attcacattt gtgatataag
     7741 attttcagca acgcctgctg gcggggaatt ttggcaatat attgcaagcc gacaatcaat
     7801 ttttcacgca attgttgaaa cgggtcatgc tctaatccag ccgtcaagtg ttcctggatt
     7861 aactcccgca atgaaggctg ttgcaaccac atctcattaa acagttgagt cttgttttcg
     7921 aagtgccagt agatagcgcc acgcgtaacg ttagcagcgt cggcaatgtc gttgagtgtc
     7981 gtcttgctta cgccatgctg cgcaaactgg gcgatggcag tttcaatcag ttcttgccgg
     8041 gtcttcagag cttcggcttt ggttcttttt gccatgatta attattcagg aaataaatat
     8101 attcgacaca gagtgagaaa atagcgaagg ttaatctatc acctaatgtg tatttatacg
     8161 agaggctaat attgagttgc tataaatcgt
//
LOCUS       DAZFKM010000186         7902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFKM010000186 DAZFKM010000000
VERSION     DAZFKM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            300..1571
                     /locus_tag="ACHKAY_004666"
     CDS             300..1571
                     /locus_tag="ACHKAY_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO5703203.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHKAY_004667"
     CDS             complement(1601..2605)
                     /gene="dppF"
                     /locus_tag="ACHKAY_004667"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO5703204.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHKAY_004668"
     CDS             complement(2602..3585)
                     /gene="dppD"
                     /locus_tag="ACHKAY_004668"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO5703205.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHKAY_004669"
     CDS             complement(3596..4498)
                     /gene="dppC"
                     /locus_tag="ACHKAY_004669"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO5703206.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHKAY_004670"
     CDS             complement(4508..5527)
                     /gene="dppB"
                     /locus_tag="ACHKAY_004670"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO5703207.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHKAY_004671"
     CDS             complement(5678..7285)
                     /gene="dppA"
                     /locus_tag="ACHKAY_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO5703208.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7637..>7902)
                     /locus_tag="ACHKAY_004672"
     CDS             complement(7637..>7902)
                     /locus_tag="ACHKAY_004672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703209.1"
                     /translation="GTALIVGCGATALFDQHTTHFPANFAPHISLSINGFVTETIVFF
                     YICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1818 a         2138 c         2024 g         1922 t
ORIGIN      
        1 ttccggcttg ccgctgattt tacgattgct ttttactgtc agcacccggc aatcttcctc
       61 attgcgcgat gccttccagc ttaatttctt tatttgcaat agttgcagtt catatccggg
      121 ctgactccag actggtagtg aaatgcgcaa ttttgtagaa ttgcgcctcc tcgcgacctc
      181 cccagtgaat ctgttgcctc attttttaca tactgcgtat tcgacttctc cacctgttgc
      241 gcaagagaaa ctgggtttat tcatttttgc gagaccgact tctttctgga caggacttta
      301 tgcagcacaa cacactatcg aaacacaatc agaaattgcc gtttacacgc tacgacttcg
      361 gctgggtttt attatgcata ggcatggcga ttggtgccgg aaccgtgctg atgccagtac
      421 aaattggctt gaagggaatt tgggtattta ttaccgcagc gatcattgct tatcctgcca
      481 cctgggtagt gcaggacatt tatttaaaaa ccctttctga aagcgattcc tgtaatgact
      541 acaccgatat tatcagtcat tacctgggga agaactgggg aattttcctc ggggttatct
      601 actttttgat gattatccac gggattttta tctactctct ctccgtggtt ttcgacagcg
      661 cctcgtacct gaaaaccttc ggtttaaccg atgccgatct ttcacaatct ctactttata
      721 aagtcgctat tttcgccgta ctggtggcga ttgcgtctgg tggtgaacga ttactgttta
      781 agatttccgg gccaatggtg gtggtcaaag tagggattat tgtcgtgttc ggttttgcga
      841 tgatcccgca ctggaatttc gccaatataa ccgccttccc gcaagcctcc gtctttttcc
      901 gcgatgtcct gcttacaatt ccattttgct tcttttctgc ggtatttatt caggtactta
      961 acccaatgaa tattgcctat cgtaaacggg aagcagataa agtactggca acccggctcg
     1021 cgctgcgtac ccaccgaatt agttatatca cgctcatcgc ggtgatcctg ttttttgcct
     1081 tttcgtttac cttctcaatt agccacgaag aagccgtttc tgcctttgaa caaaatatct
     1141 cggcactggc gctggccgcg caggtgatcc ctggacatat cattcatatc acctctacgg
     1201 tgctgaatat ctttgccgta ctgaccgcat tctttggcat ttatctcggt ttccacgagg
     1261 ctattaaagg cattattctc aatctgttaa gccgaattat tgataccaag aaaattaact
     1321 cacgcatgct gactctggcg atctgcgctt ttatcgtcat tacattgacg atttgggttt
     1381 cgtttcgtgt atcggtgctg gtgttctttc agttgggaag cccgttatat ggcattgtgt
     1441 cgtgcctcat tccgtttttc ctgatctata aagtcgcaca actggaaaaa cttcgcggat
     1501 ttaaagcctg gctaattctg ctttacggca ttttgctatg cttgtcgcca ctgttgaagc
     1561 tgattgagta aaccggagcg catggccccg gttttgtgag ttaacgctgc ggattttcat
     1621 cctgatcaac agcaaaacaa gcgaccagtt gaccgccgta gtcttttagc tgcggctgca
     1681 actgggtgca ggggccgaag cgccgacgac agcgggcgtt gaaggcgcaa cccggcggag
     1741 gattcagcgg gcttggcagt tcaccggtga gtttgatgcg ttcacgacga tcgtccgggt
     1801 tcaggcgtgg cgtcgcggag agcagcgcct gggtgtacgg atggcgcggg ttattgaaga
     1861 tttggtcctt cgttcctttc tccacgcagc ggcccaggta catcaccatc acttcatcag
     1921 caatgtgctc caccaccgac aggtcgtggg agataaagac ataagaaagc cccaactcct
     1981 gctgcaaatc catcatcaga ttcagcacct gcgcacgcac tgagacgtcc agcgcagaaa
     2041 ccggttcatc ggcaatcacc acgtccgggt cgagcatcag accacgggca atagcgatac
     2101 gctgacgttg tccgccggag aacatatgcg gatagcggtc atagtgctcg gttttcaggc
     2161 cgactttcgc catcatcgac agggcttttt cccgacgctg ttctttgctg aagctggtgt
     2221 taataagcag cggctcttcg agaatttgcc caactttttt acgcggattc agcgaaccgt
     2281 aagggttctg gaagacaatc tggattttct gccgacgcag cttctgcgcc tgcggatcgt
     2341 gcttgagcag atcctgcccc tggtaataca gctcaccacc ggtgggcgtt tcgatcatcg
     2401 tcagcaaccg accgagggtc gatttaccgc agccagattc gcccactact gccagcgttt
     2461 tgccacgttc aaggttaaac gaaacgccgt ccagcgcctt caccagacgt tccggcgcga
     2521 acatgccttt cttcaccgga taatgttttt tcaggtcgat agcctgcaac agcggttgtt
     2581 gcgaggtggc ctcttgcgta ctcatagtgt cggcctcccg gcatcatcaa gtgggtaatg
     2641 gcatttggac tgacgcccat cagcgagcat attcagcgcc ggttcttcag cgcgacatct
     2701 gtcagtggca taggggcagc gcgggttaag caggcagccg ttcgggcggt cgtacttacc
     2761 gggaacgaca cctggcaacg acgccagacg ttctttgtcc tgagcaaatt ctggcagcgc
     2821 acgcagcaat gcctgagtat acgggtgacg cggcgcatgg aagatggcgt gcgcatcacc
     2881 ggtttccacc acctggcctg catacatcac gatgatttta tgtgccgctt ccgccaccag
     2941 cgccaggtca tgggtaatta acaccagcgc catgttctct ttctgctgta gctccagcag
     3001 tagttcgatg atttgcgcct gaatggtcac gtccagcgcg gtggtcggtt catcggcaat
     3061 cagcagtttt ggccgacagg caatcgccat ggcgatcatc acgcgctggc tcatgccgcc
     3121 ggaaagctga tgcgggtaaa tatccagacg cgatgccgga tcgggaatac cgacctgatt
     3181 cagcaggtca atcgcccgct gacggcgagt gctcttatta ccgccctgat gcaccttaat
     3241 cgcttccatg atctggaaac ccacggtgta gcacgggtta aggctggtca tcgggtcctg
     3301 gaagatcatc gccacttcgg cacccaccag gttgcggcgc tctttttctg atatacgctg
     3361 caaatcctga ccgttaaaca ccagcttttc cgccattacg cggcccggat aatcaatcag
     3421 ccccataatc gccagtgaac tgaccgactt accggaacca gactcaccca caatcccgac
     3481 cacttcgccc tgttttacgc tgtagctgat gcggtctacg gcgcggaacg gtgcgctttc
     3541 gtcgccgaaa tgcaccgata atttatctac atttaataac gccatctcga acctcttact
     3601 gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc catcaggtta aatgccagca
     3661 ccgtcagcag gatcgccaga cccgggaagg tcacgaccca ccaggcgctt tgcgcgaact
     3721 gcaacacgtc ggagagcatg gtgccccact ctggtgttgg cggctgcgct cccatgccga
     3781 ggaagccaag ggcggccata tcgagaatgg cgttagagaa accgagcgac gcctgaacaa
     3841 tcagcggcgc aaggcagttc gggaagatgt taataaacat ctgacgcatc gccccggcac
     3901 ccgccacgcg agacgcggtg acgtaatcgc ggttaacttc caccagcacg gcggcgcggg
     3961 ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc cagcgcggcg ttaccaatcg
     4021 acgggccgaa gattgccacc agcaccagcg ccagcagcag acttggcagc gccagcatga
     4081 tatcgaccac gcgcataatg atgttatcga ccaggccgcc aaagtaaccg gcgatcaggc
     4141 cgagaataac gcccataatc agcgataaca caactaccag acagccaacc agcagcgaca
     4201 ggcgcgcacc gtacatcagg cgcgacagca catcacggcc tacgtcatcg gtgcccagca
     4261 agtgcgccat gctgccgcct tcctgccagg ctggcggggc gagcagtgca tcgcggaact
     4321 gttccgccgg gttatagggt gcaatccagt tggcaaagat cgcgatgaac agcacgatga
     4381 cgacgtaaac cagcccgacg accgcgcctt tgttgcgttt aaaatagtgc cagaactcct
     4441 gtaacggggt catcggcacc ggtgcgctaa tcactttatt ttcagtaacc tgtgacatga
     4501 tgtcccctta cttcttatga cgaatacgcg ggttcaccac gccgtacagc agatcgacca
     4561 gcaagttgac gaggataatc atcgtcgcca ccagcaatac gccgccctgc actaccggat
     4621 agtcgcggcg ttgcagcgcg tcaatcaacc agcgccccag accgggccac gagaagatgg
     4681 tttcggtcag aatcgccccc gccagcaatg ttcccacctg caggccgata acggtcacta
     4741 ccggcagcat cgcgttacgc agcgcatgga cgataatcac ccgcatgcgg gttagccctt
     4801 tggcgcgcgc ggtgcggatg taatcctcgc ccagcacttc cagcatcgag gagcgtgtca
     4861 tacgcacaat gaccgccagc ggaatagtac ccagcacaat ggcaggcaag atcatatggg
     4921 cgacggcatc gataaagttg ccgtcttcac cccagatggc ggtgtcgatt agcataaaac
     4981 cggttaacgg attggagtca tcgaggaaca ccatatcgct cacgcgaccg gagacgggcg
     5041 tcaggttcca gtgcaccgaa accagcatga tcagcatcat gccccaccag aagatcggca
     5101 tcgagtagcc ggtcagcgcc aggccaaccg ctgtgtgatc gaaaatggaa ccgcgtttaa
     5161 ccgcagccag cacgccgacc ggaataccga ccgcagtcgc aaaaatcatc gcgcagacgc
     5221 caagttccag cgtggcctgg aggcgcggca cgaactcttc ccaaaccggg atgcggcttt
     5281 tcattgaaat gcctagatcg ccatgcataa cgccccaaat gtaatggaga tactgctgcc
     5341 acatcggttt atctaagccg agttcagcca gcagctgcgc gtgacgctct ggggagatcc
     5401 cacgttcgcc cgccatgatc atcaccggat cgcccgggat catgtggaca aaggcaaatg
     5461 tgagaagggt aataccgata aacgtgggga tgacgagtcc caaacgtcgg agaataaact
     5521 gcaacataac ccggattctc tatagtgacc cgcagcctgg aactgcgtgt ctgtattgct
     5581 cacaaatcaa ttcccgccta ggtcgggctg ggaatgaagt attgctgccg gatggcgctg
     5641 cacgcagagg cacagcgcca ccgggtatgg cttttaatta ttcgatagag acgttttcga
     5701 agtgatgttt gcctaatgga tcaaccacat agcctttaac ttctttgcgt accggttcaa
     5761 acacggtgga gtgagcgatg atcagtgccg gagcctgatc gtgcatcacc acctgcgctt
     5821 gcttgtacag ttcaacgcgt ttgttatggt cgtcggtagc acgcgccggt tgaatcagat
     5881 cttcaaacgg tttgtagcac cattttgagt agttggagcc ttgttcagag gcggcgcagc
     5941 tgaacagggt ggcgaagaag ttatccggat ccccgttatc gccagtccag cccatcatta
     6001 ccgtctggtg ctcgccatct ttcgcacgct tgaggtactc accccattcg taggtgacaa
     6061 ttttggcctg cacgccgact ttcgcccagt cagcctgaat catctccgcc atgcggcgag
     6121 cgttcgggtt atatgggcgc tgtaccggca tcgcccacag gtcgatggag aaaccttttt
     6181 ccagacccgc ttctttcagc aaggctttcg ctttttcagg atcgtaggtg taatcctgaa
     6241 cgtcgtcgtt atagccccac atggttggcg ggatcaggtt tttcgctgat acacccgcgc
     6301 cctgataaac cgctttgatg atcgcgtctt tgttcaccgc gtaggtcagt gcctggcgaa
     6361 ctttcacgtc atccagcggt tttttctgca cgttatagga gaggtagccg acgttcagcc
     6421 ccggcatttc catcaggttg atggatttat cctgcttcat gcgagcgatg tctgccgggt
     6481 tcgggtacgg catcacctgg cattcattct tctgcaattt cgcataacgc acggaagcgt
     6541 caggagtaat agagaagacc agcgtatcga tctgcggttt ggtgccccag tagccatcaa
     6601 acgctttgta gcggatacgg gagtcttttt ggtactgctg taactggaac ggaccggtgc
     6661 cgattgggtt gaggtccagt ttttccggtg taccggcttt catcatcgcg tcagcatatt
     6721 cttttgacag aatagaggcg aagtccattg ccaggtcagc gaggaacggc gcttccgggc
     6781 gagtcagcac aaactgaacg gtgttgtcgt ccaccttttt cacttcgctg atcagctccg
     6841 gcaagcccat gccttcgaag tattcgtagc tgccgccaga aactttatgg tacgggtttt
     6901 gcgcgttttt ctgacgatca aacgagaaca ccacgtcatc ggcgttcagt tcacgcgtcg
     6961 gtttgaattc tttattgtcg tgccacttca cacctttacg cagatggaag gtataggttt
     7021 taccgtcttc gctgacttcc cacttttcag cgaggcccgg gatcacttca gtggtgccga
     7081 ttttaaattc aaccaggcgg ttataaagcg gtacggaaga ggcgtcatag gtggtgccgg
     7141 aggtaaacag ctgcgggtta aacccttccg gagatccttc tgagcaataa accagagttt
     7201 tagcctgaac acttgctgcg acggtcatag ccaccaggct gagaccaagc ttcagcatcc
     7261 ctgacttttt caaggaaata cgcattattc tgctccaatt gtgatgtttg ttgttttaac
     7321 cctttgcagt gggttgtcgc tgcctgacct tttttgtttt ttgcccggtc gggtcaacgt
     7381 tatgaggtgg ggatgccgta ctaattaaca tcagtgtaac agtgcggatc ctccataaaa
     7441 tgcccctcgt gacctacaat ctgtcaacag aatgtgaaaa cgtcaataca gccaaccggg
     7501 atttacacca acggtgagaa tccacaaaca aagattaaaa aaacttcaaa cagctatttg
     7561 cagcagagaa atttgtgcta ctccaacatg accagaacaa tcagcttaat atttagcaac
     7621 atttggtgat taagttttat gcatttaatg aaaaaaatct gaggaaaagg tggatatctg
     7681 agcgattaat accatgaacg ctaaaacgca cagcggaaaa tgccagcgcc agccataagt
     7741 aacgcaaaaa aagatttaag caaatataaa aaaagacaat ggtttctgtc acaaatccgt
     7801 taatggatag tgagatatgg ggcgcaaagt tggcaggaaa atgtgtggtg tgttggtcga
     7861 ataaggcggt cgcgccgcat ccgacaataa gtgcggtgcc gt
//
LOCUS       DAZFKM010000187         7891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFKM010000187 DAZFKM010000000
VERSION     DAZFKM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..209
                     /gene="kdpF"
                     /locus_tag="ACHKAY_004673"
     CDS             120..209
                     /gene="kdpF"
                     /locus_tag="ACHKAY_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO5703210.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            209..1882
                     /gene="kdpA"
                     /locus_tag="ACHKAY_004674"
     CDS             209..1882
                     /gene="kdpA"
                     /locus_tag="ACHKAY_004674"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO5703211.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1905..3953
                     /gene="kdpB"
                     /locus_tag="ACHKAY_004675"
     CDS             1905..3953
                     /gene="kdpB"
                     /locus_tag="ACHKAY_004675"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO5703212.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3962..4534
                     /gene="kdpC"
                     /locus_tag="ACHKAY_004676"
     CDS             3962..4534
                     /gene="kdpC"
                     /locus_tag="ACHKAY_004676"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO5703213.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4527..7211
                     /gene="kdpD"
                     /locus_tag="ACHKAY_004677"
     CDS             4527..7211
                     /gene="kdpD"
                     /locus_tag="ACHKAY_004677"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO5703214.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7208..7885
                     /gene="kdpE"
                     /locus_tag="ACHKAY_004678"
     CDS             7208..7885
                     /gene="kdpE"
                     /locus_tag="ACHKAY_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO5703215.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1600 a         2065 c         2358 g         1868 t
ORIGIN      
        1 aaatcatggc ttttgccatt tttatacttt ttttacaccc cgcccgcaga tttttgcgaa
       61 atctttgcag ccagaattct acccttccgg tatcactttt aggccactgg aggtgcactg
      121 tgagtgcagg cgtgataacc ggcgtattgc tggtgttttt attactgggt tatctggttt
      181 atgccctgat caatgcggag gcgttctgat ggctgcgcaa gggttcttac tgatcgccac
      241 gtttttactg gtgttaatgg tgctggcgcg tcctttaggc agcgggctgg cgcggctgat
      301 taatgacatt cctcttccag gtacagcggg cgttgaacgt atactctttc gtttacctgg
      361 cgtctctgac catgagatga actggaagca atatctttgt gccattctcg gcctgaacat
      421 gctggggctg gcggtgctgt tttttatgtt gctcgatcag cactatctgc cgcttaatcc
      481 acagcagttg ccggggctgt cgtgggatct ggcgctgaat gccgccgtca gctttgtcac
      541 gaataccaac tggcaatctt acagcggtga aaccacgttg agctatttca gccagatggc
      601 tggcttaacg gtgcaaaact ttctttctgc cgccagcggg attgcggtga tttttgccct
      661 catccgtgcg tttacccgcc agagcatgag cacactcggg aatgcctggg tcgatctgct
      721 acgcatcacg ttatgggtgc tcgtccctgt ggcgttgttg atcgcactgt tttttattca
      781 acaaggtgcg ctgcaaaact ttcagcctta tcaggctgtg aataccgttg aaggagcgca
      841 acagctgtta cccatggggc ctgtagcttc tcaggaagcg atcaagatgc tcggtactaa
      901 cggcggtggc ttctttaatg ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac
      961 caacttcgtg cagatgctgg cgatcttctt gatcccaacg gcgctgtgct ttgcctttgg
     1021 tgaagtgacg ggcgatcgcc gccaggggcg catgttgctg tgggcgatgt cagtgatttt
     1081 tgtcatctgc gtaggcgtgg tgatgtgggc agaagttcag ggtaatcctc atctgctggc
     1141 gctcggcgca gacagcagca tcaatatgga aggtaaagag agccgtttcg gcgtgctggt
     1201 cagtagcctg tttgcggtcg tgactacggc ggcttcctgt ggcgcggtga ttgcgatgca
     1261 tgattcgttt accgctctcg gtggcatggt gccgatgtgg ctgatgcaaa ttggtgaagt
     1321 ggtgttcggc agtgtcggtt ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt
     1381 gtttattgcc gggctgatga ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt
     1441 acgcgagatg aaactgactg cactggcgat tctggttacc ccgacgctgg tgctgatggg
     1501 cgcggcgctg gcaatgatga ccgacgccgg acgtagcgcc atgctcaacc ctggcccgca
     1561 tggttttagc gaagtgctgt acgccgtgtc atccgccgct aacaacaacg gcagcgcctt
     1621 tgccggatta agcgccaact ccccgttctg gaactgttta ctggcgttct gcatgtttgt
     1681 cggtcgcttc ggggtgatta tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa
     1741 gagccaaccc gccagctccg gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt
     1801 aatcggcacc gtgttgctgg ttggcgcact gacctttatc cctgccctgg cgcttggtcc
     1861 ggtggcggaa tatctctcct gatgatattg agtgagcact gaatatgagt cgtaaacaac
     1921 tggcgctatt cgaaccaaca cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa
     1981 acccgcaggc gcaatggcgc aatccggtga tgtttatcgt ctggatcggc agtctgctga
     2041 ccacctgtat tagcatcgcg atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg
     2101 cggcaattag cggctggctg tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg
     2161 cagaaggccg cagtaaagcg caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg
     2221 cccgcaagct gcgtgagccg aaatatggcg ctgcggcgga caaagttcct gccgatcaac
     2281 tgcgtaaagg tgacatcgtg ctggtagaag ctggcgatat tatcccctgc gatggtgaag
     2341 tcattgaagg cggcgcatcg gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga
     2401 tccgtgaatc cggcggcgat tttgcctccg tcaccggcgg cacgcgtatt ctttctgact
     2461 ggctggtgat tgagtgtagc gttaaccccg gtgagacatt tctggatcgg atgatcgcga
     2521 tggtggaagg cgcacagcga cgcaaaacac cgaacgagat tgccctgact atcctgctga
     2581 ttgctctgac catcgtcttt ttactggcaa ccgccacgct gtggccgttt tccgcgaggg
     2641 gcggtaatgc cgtgagtgta acggtactgg tggcgctgct ggtctgtctg atcccaacca
     2701 ctattggcgg cctgttgtca gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga
     2761 atgtgattgc caccagcgga cgtgcagttg aagcggcagg tgacgttgac gttctgctac
     2821 tggataaaac cggcaccatc acactcggta accgtcaggc gtcggagttt atccctgcgc
     2881 agggcgtgga tgaaaaaacg ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa
     2941 cgccggaagg ccgcagtatt gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg
     3001 atgtgcagtc gctccatgcc acctttgtac cgtttactgc gcaaagccgg atgagcggga
     3061 tcaacatcga caaccgcatg atccgtaaag gttctgtcga tgccattcgc cgccatgttg
     3121 aggctaacgg cggtcacttc cctaccgatg ttgatcaaaa agtcgatcag gttgcgcgtc
     3181 agggggccac gccgctggtg gtggtggaag gttcccgtgt gctgggcgtt attgcgctga
     3241 aagatatcgt caaaggcggt attaaagagc gcttcgccca gctgcgcaaa atgggcatta
     3301 aaacggtgat gattaccggc gataaccgtc tgactgccgc cgccattgct gcggaagcgg
     3361 gcgtcgatga ttttctcgcc gaagcgacac cggaggccaa gctggcattg attcgtcagt
     3421 atcaggcgga aggccgtttg gtggcgatga ccggcgacgg caccaacgat gctccagcgc
     3481 tggcgcaggc agatgtcgcg gtggcgatga actccggtac tcaggcggcg aaagaagcgg
     3541 gcaatatggt cgatctcgac tctaacccga ccaagttgat cgaggtggta catattggca
     3601 aacagatgct gatgacccgt ggctcgctga ccaccttcag cattgccaac gatgtggcga
     3661 aatacttcgc cattattccg gcggcgttcg cggcaacgta tccgcagtta aatgcgctga
     3721 acatcatgcg ccttcattcg cccgactccg ccattctcag tgcggtcatt ttcaacgcct
     3781 tgattatcgt ctttttgatt cctctggcgt taaaaggcgt gagttataaa ccgcttaccg
     3841 cttctgccat gttgcgccgt aacttatgga tttacggtct gggtgggctg ctggtgccgt
     3901 ttattggtat caaagtcatt gatttactgc tgaccgtttg cggtctggtg tgaggtttac
     3961 catgcgtggc ttacgtccgg cattatcgac atttatcttt ctgttattga ttactggcgg
     4021 cgtttacccg ctgctgacca ccgtgctggg gcaatggtgg tttccctggc aggccaacgg
     4081 ttcgttgatt cgtgaaggtg atacggtgcg gggttcggca ttaatcgggc agaattttac
     4141 cggcaacggc tattttcagg gtcgcccgtc ggcaacggca gaaatgccct ataatccaca
     4201 ggcttctggc gggagcaatc tggcggtcag taaccctgag ctggataaac aaatagccgc
     4261 acgcgttgct gcattacggg ccgctaaccc ggatgccagt acgaacgttc cggttgaact
     4321 ggtgacggca tcggcaagcg ggctggacaa taatatcacc ccgcaagcgg cggtctggca
     4381 aatcccacgc gtggcgaaag cgcgtaatct cagcgttgaa cagctcacgc aactgatcgc
     4441 aaaatacagc caacaaccgc tggtgaaata tatcggccag ccggtcgtca acattgttga
     4501 actcaatctg gcgctggata aacttgatga ataacgaacc cttacgtccc gaccccgatc
     4561 gtctgctgga acaaactgcc gcgccgcatc gggggaagct gaaagttttc ttcggtgcct
     4621 gtgcaggcgt cgggaagacc tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag
     4681 ggctggatat tgtggttggc gtggtagaaa cccatgggcg gaaagatacc gccgccatgc
     4741 ttgaagggct ggctgttctg ccgcccaaac gccaggcgta ccgtgggcgg catatcagcg
     4801 agtttgatct cgatgccgcc ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg
     4861 cgcacagtaa tgcgccaggt tctcgtcatc ctaaacgctg gcaggatatc gaagaactgc
     4921 tggaagctgg cattgatgtt ttcacaaccg tcaacgttca gcatctggaa agtctgaatg
     4981 atgtggtcag cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat ccttttttcg
     5041 atgccgccga cgacgtagtg ctggtggact tgcccccgga cgatctgcgc cagcggctga
     5101 aagaaggcaa agtctatatt gccgggcagg cggagcgcgc cattgaacat tttttccgca
     5161 aaggcaatct gatcgccctg cgtgaactgg cactgcgccg tactgccgat cgcgtagatg
     5221 agcaaatgcg cgcctggcgg gggcatcctg gtgaagagaa agtgtggcac acgcgcgacg
     5281 cgatcctttt atgcatcggg cataacaccg gcagtgagaa actggtccgc gcggcggcgc
     5341 ggttggcctc gcgcctgggt agcgtctggc acgcggtgta tgttgaaacc cctgccctgc
     5401 atcgcttacc ggaaaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc
     5461 tgggcgcgga gacggctaca cttactgatc cagcggaaga aaaagcagta gtgcgttatg
     5521 cccgtgaaca taatctcggc aagattattc tcggtcgccc ggcctcgtgc cgctggtggc
     5581 gtcgggaaac gtttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg
     5641 tcgcgcttga tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctctttta
     5701 aagacaagtg gcgggtacag attcagggat gcgtggttgc cgccgcgtta tgcgccgtta
     5761 tcactttaat tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt
     5821 atctgcttgg cgtggtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcta
     5881 ccgtcattaa tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgctg
     5941 tctctgatgt gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg
     6001 ggaaccttac tgcgggtgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca
     6061 cacggcactt atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg
     6121 ccgccaccag cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc
     6181 ccgatgacaa cggtaaattg cagccgttaa cacatccgca aggaatgacg ccatgggacg
     6241 atgccatcgc gcagtggagt tttgataaag gcctgcctgc gggtgcgggc accgacacgt
     6301 tacccggcgt gccgtaccag attttgccgc taaaaagcgg cgagaaaacc tacggactgg
     6361 tggtggtgga accggggaat ctgcgtcagt tgatgatccc ggagcagcag cgcctgctag
     6421 agacgtttac gctgttagtc gccaatgcgt ttgagcggct gacgctgacc gccagtgaag
     6481 aacaggcgcg gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcggcgcttt
     6541 cgcatgattt acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg
     6601 atctggcaag cgaaggatcg ccccacgccc gccaggccag cgagattcgt cagcatgtgc
     6661 tgaacactac ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct
     6721 ttaatttgaa gaaagagtgg ttaacgctgg aagaagtagt cggcagcgct ctgcaaatgc
     6781 tggaaccggg tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc
     6841 acgttgacgg gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat
     6901 atgcgggtgc gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac
     6961 tggatgtctg ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata
     7021 agtttgctcg cgggaataaa gagtcggcag taccgggggt agggcttgga ctggcaattt
     7081 gtcgggcgat agtggatgta cacgggggca ctattaccgc gttcaaccga ccggaaggtg
     7141 gtgcctgttt tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg
     7201 aggatatgtg acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg
     7261 cacggcgctg gagggcgacg ggatgcgcgt ctatgaggcc gaaacgctgc aacgcggctt
     7321 gctggaagcg gctacccgta agccagattt gattattctc gatctcggcc tgcccgatgg
     7381 tgatgggatt gagtttatcc gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct
     7441 ttccgcacgc agcgaagaga gcgacaaaat cgccgcgctg gatgccggag cggatgatta
     7501 tctgagtaag ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg
     7561 ccactccgcc accgccgcgc ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt
     7621 agccgcccgc gtgattcacc ggggtgagga agaggtgcat ctcacgccga ttgagttccg
     7681 cctgctggcg gtgctgctaa ataatgccgg gaaagtgctc acccagcgcc agttactcaa
     7741 tcaggtgtgg gggccaaacg cggtcgaaca cagtcactat ttgcgtattt atatgggaca
     7801 tctgcgacaa aaactggaac aggatcccgc tcgcccccgc catttcatta ctgaaaccgg
     7861 tattgggtat cggtttatgc catgaatgtt t
//
LOCUS       DAZFKM010000188         7870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFKM010000188 DAZFKM010000000
VERSION     DAZFKM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..583
                     /locus_tag="ACHKAY_004679"
     CDS             59..583
                     /locus_tag="ACHKAY_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO5703216.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            667..1353
                     /gene="lpfB"
                     /locus_tag="ACHKAY_004680"
     CDS             667..1353
                     /gene="lpfB"
                     /locus_tag="ACHKAY_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO5703217.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1377..3908
                     /gene="lpfC"
                     /locus_tag="ACHKAY_004681"
     CDS             1377..3908
                     /gene="lpfC"
                     /locus_tag="ACHKAY_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO5703218.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3919..4974
                     /gene="lpfD"
                     /locus_tag="ACHKAY_004682"
     CDS             3919..4974
                     /gene="lpfD"
                     /locus_tag="ACHKAY_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO5703219.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4980..5504
                     /gene="lpfE"
                     /locus_tag="ACHKAY_004683"
     CDS             4980..5504
                     /gene="lpfE"
                     /locus_tag="ACHKAY_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO5703220.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5757..7448
                     /gene="eptB"
                     /locus_tag="ACHKAY_004684"
     CDS             5757..7448
                     /gene="eptB"
                     /locus_tag="ACHKAY_004684"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO5703221.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7540..7616
                     /locus_tag="ACHKAY_004685"
     tRNA            7540..7616
                     /locus_tag="ACHKAY_004685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7574..7576,aa:Pro,seq:cgg)
     gene            7651..7784
                     /locus_tag="ACHKAY_004686"
     ncRNA           7651..7784
                     /ncRNA_class="other"
                     /locus_tag="ACHKAY_004686"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2048 a         1813 c         1994 g         2015 t
ORIGIN      
        1 ctgcgccccg ttgtatgaac aacatcaagc atatttatct atataaatgg agttataaat
       61 gaaaaaggtt ctgtttgcct tatctgcgct tgcgctgacc tctacttccg tattcgcagc
      121 tgatgcgggc gacggtactg ttaaattcac cggtgagatt gttgacgccg cttgtgttgt
      181 gtctccagac actcaaaaac aagaagttgt tctgggtcag gtgaacaagt ctgtatttac
      241 tgctatgggt gataaatcca cagcaacgcc gttcaaaatc aaactggaaa actgcgatat
      301 ctccactttc aaaaatgtgg aaatcagctt taacggcgtt ggcgatgcag acaacagcaa
      361 actggtttct gtaagcactg aaccaggtgc cgcaactggc gtaggtatcg gtatttatga
      421 taacaccaat accctggttg acctgaatac tggtaaatct gctactgtcc tgaaagaagg
      481 tcagaccgtt ctgtatttca ccgctaacta tgtctctacc aaagatgcag taaccacggg
      541 ttacggtaac gctgaagtcg acttcaacct gacttacaac taatcgatcc tgacggaacg
      601 atcatctctc aatggcaaca gagactgtgt tgccattatt cacaccaatg actcagggac
      661 cgaatcatga aacgtattag ctttgctgtg ctggcacttt taatgaccgc acaacagtgt
      721 tttgctggtg gcgtagcctt aagcacgacg cgtgttattt atgatggcgg caagaaagaa
      781 gcgtcgctga ccgttgaaaa ccataataaa aatgaagagt ttttgatcca gtcatggatt
      841 gatgatgtga atggcaataa aaaaacaccg tttattatta caccgccatt attcaaactt
      901 gatcctgata aaaataacgt cttacgtatc gtcaatattg aagatacctt acctaaagat
      961 cgtgaatccg tttactggat taatgtaaag gccattcctg cgaaaagtgc tgaaaatgaa
     1021 aataaaaacg tcttacagat agccgttcgc acccgtttga agttgttcta tcgtccaaaa
     1081 ggtttgccgg gaagcaacct cgaggccatg aaacagttac gtttccagcg tcaaaataac
     1141 caaattgtgg ttaataacga ctccgcttat aacctgacct ttaatcagtt ttccattaat
     1201 ggacaaaaga ttgaacgggc gggaatggtt ctcgcgaaag gaaagttaaa cattaacctt
     1261 ccagccggta caggcaacgc gcacgaagtg aaatacagca ttattaatga tttcggtgtt
     1321 gctggcgaaa tgctgacgaa gccgattaac tgatcctttt acaaggcgct tttactatgc
     1381 attggatgca tttacctctc tatcactaca gagcgcattt ttccttctct ctgctggcat
     1441 taacgattgc cagctcgtta cctgcgtatg gcggaaaatt taaccctaaa tttctcgaaa
     1501 acgttcaggg tatcgatcaa cacatcgact tgtcggtata cgactcacct gtcggccagc
     1561 aaatccccgg taaataccgg gtttcggtgt ttgtaaacga agaaaaaatg gcttcccgga
     1621 cgctggattt tagtaccgcc agcgaagcga aacgcaaagc cagcggtgag tcgcttatgc
     1681 cttgccttag ccgggtgcaa ctggaagaaa tgggcgtgcg cgttgacagc tttccggcgc
     1741 tgaagatgtc accaccagaa gcctgcgtag cgtttgatga aatcattccc caggccacca
     1801 gccgctttga ttttaatact cagacgctgc atctttcctt tccacaggcg gcaatgatga
     1861 tgaccgctcg cggcacggtt gatccttctc gctgggacga aggaatacct gcgctgttgc
     1921 tggattacag ctttagtggc agcaatggac gcaatgaagg tacaggttca tcttcagatt
     1981 cgaccagtga cagttattac ctgaacctgc gcagcggttt gaacgttggt ccgtggcgtt
     2041 tacgtaataa cagtatctgg aaccgtaccg atggcaaaaa ccaatgggat aacgttggta
     2101 catcgttaaa ccgggcaatt attccactta aatcacagat aactctgggt gataccgcca
     2161 cgcctggcga gatttttgat agcgtacaaa tgcgtggggc gttactggca tccgacgatg
     2221 aaatgttgcc ggacagccag cgtggctttg ctccggtagt gcgcggcatc gccaaaagca
     2281 acgctgaagt gagtatcgaa cagaacggct atgtcattta tcgcactttt gttcagccgg
     2341 gtgcatttga gattaacgat ctctacgcaa cctccggcag cggcgacctg acggtgatca
     2401 ttaaagaagc cgacggaagt gaacaacgtt ttatccagcc gttttcggcg gtggcgattt
     2461 tccagcgtga agggtatctg aaatacagtc tggctgccgg tgaatatcgt gctggtaatt
     2521 acgacagcga taaaccacat ttcgggcagt ttaccgccat gtacggcttg ccgtggggta
     2581 tgaccgccta cggtggtgca ttgttgtcgg cagattacaa cgctttagct ctgggcctgg
     2641 ggaaaaactt cggtaccatt ggcgcagtat cggtggatgt cactcaggcg aaaagtcagt
     2701 tacgcaacaa tgaaaaagag gaaggccagt cttatcgctt cctctattcg aaaagttttg
     2761 agggtggcac tgacctgcgt ctgttgggct acaaatactc aaccagcggc tactacacct
     2821 tccaggaagc aactgatgtt cgcagtgacg ccgacagtga ttaccgccgt tatcacaagc
     2881 gcagccagat acagggcaat attacccagc aactgggaga ctacggctcg gtttatttca
     2941 atatgacgca gcaggattac tggaacgttg acggtaagga aaactcctta agcgccggtt
     3001 atcacggtca cattggtcgg gtgaattaca gcgtggccta tacctggacg agaagtccgg
     3061 aatgggaaga agacgatcgc ttgtggtcat tcagtgtttc catccctctg gggggcgcgt
     3121 ggagcagtta tcgcatgact accgaccaga atggcaagac gtcgcaacag gcctctgtta
     3181 gcggcacgtt actggaagat cgtaacctga gctataacgt tcagcagggt tacaccagta
     3241 atggcgtagg ctacagtggc agcgtcaaca tggggtatat gggtggctct ggtaacattg
     3301 acgtaggcta taactattct aaagataacc aacaggttaa ctatggcgtg cgcggcggtg
     3361 tcatcgttca cagtgaaggt ataacgcttt cgcagccttt aggcgaaagt ctggcgattg
     3421 tttctgcacc gggcgcgcgt ggtggacatg tggttaacag cagcggcgta gaagttgact
     3481 ggatgggcaa cgccgtggtg ccttatctca caccatatcg tgaaaccatt gtggaattgc
     3541 gtagcgacac gttggggcaa aacgttgaac tacaggaagc gttccagaaa gtcgtgccta
     3601 ctcgtggtgc agttgtgcgt tcccgctttg atacccgcgt cggttaccgt gtgctgatga
     3661 gcttaaaaca ggcgaatggc aacgcagtac cgtttggtgc aaccgcagcg ttaattgatg
     3721 agagcaaacc tgccagcagc attgtgggtg aagaaggaca gctttatatc agcgggatgc
     3781 cagaagaggg cgaattgcag gtgagctggg ggaacgagca ggcgcaacgt tgccgtgtgc
     3841 cattccgctt gccagagaac aaggacaatg cggctatcgt gatggttaat gcggtatgcg
     3901 agaaataagg atatttagat gaaaattaaa aatttgttgg caggtatggt gctgttaggg
     3961 acgagcactt ttgctggaaa tatatgggct gcggattggg gaccatgtca gacatcaaat
     4021 ggggtggccc atgagtatag ttttgatttc gtacagacta tccaggtacc ttcagaaaat
     4081 aaggctggga aaatattaac tcagcccttt gctttaggta caaaatattc cgcttattgt
     4141 gaatgcccgg atcccattcc agataatgga gttgtgactt attttaaagg tgtaacgctt
     4201 ctgcctgagc ctggaactgt agatgggtac tataaattca ataattacat tgatattctc
     4261 accaaaatat atgtctacac acaattagat atcccggtgc cttttaccga tcgaagcaat
     4321 ggcacggcac agaaagaatg tacgccctat actgctaaca attgggggac aggtggtaaa
     4381 ggaagtatca gtatttatat ttcacatcct tttgttgggc aaatgattat tcctaaaaca
     4441 cgagttgcgt ccctctttgg gacaaaaaag aaaggtgttt acaatgatag tcagcccatg
     4501 gccaatgtca gtatttccgg gagtattaca gttactcaag gctgtgaact tgccgctggc
     4561 accgtactgg atattccatt cggagaatat caggcccatg attttaaagg gcgtgccgga
     4621 caaccaccac aaaatgttca gaaggtacaa aaagaattaa gctttgactg caataatatt
     4681 tcagatggcg tcaaaattta tctcagtatt gatgcaacac caaatactgc ctatcccagc
     4741 gcaattgatt taggcaacgc cgatgttggt gcagtcattg aggacggtaa aggtaatatt
     4801 cttaagccga atgacagtaa tagcttatta gaaatgaacc caggctcgct gtatgaagat
     4861 gtcaaacgca aagcgacaac cacaattacc gcttatcccg ttagcactac cggaaaatta
     4921 cccgctgccg gggattatag cggtattgct accatacatg tcgaactgga gtaacagtca
     4981 tgaagcgaac aatacttgcg ttatgcctgc tgcctctttc cttttcgtca ttcgcggcaa
     5041 ccacggattt agggccgaaa ggaacattga aattcaccct taaaatcagc cagggtgctt
     5101 gcgaactggt gaaagacagc gttgaggttg acatgggcac cgcggttctc aaaaagccgg
     5161 taagaatcgg aacggaaatt aatcccactc ctttttccat cggcctgaaa aactgtagcg
     5221 aagtggtcag agcctacgtg actatggacg gtacacccaa tgcagacgac tcaaatcttt
     5281 ttgcgctgga tgcgggtggc gctacaggcg ttgggctgaa aatcaaaacc gctgctggag
     5341 tacaacagtt cccggaaaat acaacgccga caccggttga gtttgcaatt aacggtgatg
     5401 gcgcacatca actgaactat atcgccagct acgtgcctat tcgcgccgat gcaactattg
     5461 gtcgcgcaga tgcaacggtc gactttactg tacagtacga ataatttcac atcttaaggg
     5521 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5581 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5641 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5701 ggcctgaaaa gccgctaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     5761 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     5821 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     5881 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     5941 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6001 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6061 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6121 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6181 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6241 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6301 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6361 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6421 atgaatcttc cgataataat tcattgctta atccggcgaa gaaattcact tatcaggcac
     6481 cgcaaaacgt tgatgacact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6541 atatgggcat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6601 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6661 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6721 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     6781 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     6841 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     6901 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     6961 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7021 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7081 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7141 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7201 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7261 aaaatccggc caatgctcag gcatttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7321 cacgccgtca tgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7381 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gtggcagcgg
     7441 ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7501 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcctggt
     7561 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7621 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7681 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7741 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     7801 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcgggg
     7861 aggttcgact
//
LOCUS       DAZFKM010000189         7820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFKM010000189 DAZFKM010000000
VERSION     DAZFKM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            438..1187
                     /locus_tag="ACHKAY_004687"
     CDS             438..1187
                     /locus_tag="ACHKAY_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO5703222.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            1114..3236
                     /locus_tag="ACHKAY_004688"
                     /pseudo
     CDS             1114..3236
                     /locus_tag="ACHKAY_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3406..4458)
                     /locus_tag="ACHKAY_004689"
     CDS             complement(3406..4458)
                     /locus_tag="ACHKAY_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO5703223.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4773..6089
                     /gene="shiA"
                     /locus_tag="ACHKAY_004690"
     CDS             4773..6089
                     /gene="shiA"
                     /locus_tag="ACHKAY_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO5703224.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            6191..7645
                     /gene="amn"
                     /locus_tag="ACHKAY_004691"
     CDS             6191..7645
                     /gene="amn"
                     /locus_tag="ACHKAY_004691"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO5703225.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2143 a         1824 c         1746 g         2107 t
ORIGIN      
        1 aaaaatacaa ccaacaaaca atcctatata caaggcacat ctccagaata taaaagcaca
       61 gacaaacaac ctaaaaaaca accccataca tataaattat tttataggta aaatagatta
      121 tatattctca tagctaccat aaactatact agcctgaact atatttattc tgctgcaatc
      181 aatgcataca taacacaaat atcactcagg tacactactc aaaccacgct gggatttttc
      241 ctcaagttat aatcatcacc ccgaaaatca ttcggcattt actcattaaa tagtcacccc
      301 ataggcctgt acatgtttac tcagaaatat acatcctttt ctctgtcata aaccctctga
      361 ttaatcataa ataaatactt gtgacaccaa tctttttcct taacggaacg aattgttgtg
      421 tagaaggaga taatattatg tcaaaaaaat atcagctatt gcttataact cattatatgt
      481 caacatgggt cactataacg gaagcagttg agatcactac caaagccata aaacaaaaaa
      541 ttactcctag cgatatttat cgtcatgcct tgagtggcaa catcctacta ttggtctatt
      601 tccagtctcc tgtgatactt aagaagatac aaacttttaa tggaaaaata aaattcaggc
      661 agtttgtggg agacctactt gataaactat gcatgcttga cagggacggt tttatttatg
      721 gtcaaaattt aagactctgt acagaagcca gatatatttg tcctgtgcaa caaattatag
      781 atacccccct gttacgcaaa ctcaaccagt ttcgtacgtt tgttcgtaat gtccgcccgg
      841 gtgatgaact ggatgtccag gcacaagtta gtgaaaaaaa tttaaccccg ccgccgggta
      901 acagcagtgg caaccttgag caacagatag ccagtacttc acagctaatt ggatctctgc
      961 tcgccgaaga tatgaacagc gagcaagcgg caaacatagc acgtggatgg gcctcttctc
     1021 agacttcagg ggtaatgaca gactggttaa gccgcttcgg taccgcaaga atcacgctgg
     1081 gcgtggatga agatttcagc ctgaagaact cccgtgacgg caaccctcga aaatggcgat
     1141 tcgatgcaac aaacagtgaa ctatgtgccg aacgtcacga atgctgaaat cacgctggca
     1201 gccttgaagg atccggtgat tgccgacaat aacgatctca cgacactaac agcaccgtcg
     1261 ctgatacaga gggcaatgcg atagccaaca ctgaggtaac atttactcta ccggaagatg
     1321 tgaaggcgaa cttcacgctg agcgatggcg acaaagcggt tactgatgcc gatggcaaag
     1381 cgaaagtcac gctgaaaggt acaaaagcag gcgctcatac cgtgacagca tcgatggttg
     1441 gcggtaagag tgagcagtta gtggtgaact ttactgcgga tacgctcact gcgcaggtta
     1501 atcttaacgt taccgaggac aatttcatcg ccaataacat tggaatgacc aggctacaag
     1561 caatagtgac tgatggaaac ggcaacccag tagaaggtat aaaagttaat ttccgtggaa
     1621 cctccgtcac gctaagcagc accagcgttg aaacggatga tcaggttttt gctgaaattc
     1681 ttgtgacaag caccgaggtc ggactaaaaa cagtttcagc ctctctggca gataaaccta
     1741 ctgaagtcat ctcgcgatta ctgaatgcca aggtagatgt taattctgcg acgattacca
     1801 gtcaggagat acctgaaggt caggtcatgg tcgcacaaga catagcagtt aaagctcacg
     1861 ttaacgacca gtttggcaac ccggtgacgc atcaagccgc gacattcagt gcagcgccat
     1921 cctcgcaaat gatcatcagc cagaacacgg tctctactaa tacgcaggga gtagccgagg
     1981 tcaccatgac gtctgaaaga aacggctcgt atactgtaaa agcatcccta gcgaatggag
     2041 cttcacttga gaaacaactg gaggctattg atgaaaaact gacactcacg tccagtccgc
     2101 ttatcggtgt caatgctcct aaaggcgcta ctctgacggc aacgctaacc tctgcaaatg
     2161 gcactccagt ggagggtcag gtcatcaact ttagcgtaac gctagaaggt gcgacgttaa
     2221 gtggcggaaa agtgagaacc aactcttcag gtcaggctcc agtcgttctg accagcaata
     2281 aagtcggtac atatacggtg actgcatcgt tccataacgg cgtcacaata cagacacaga
     2341 caaccgtgaa ggttactggc aacccaagca ccgcacatgt tgctagcttt atcgctgatc
     2401 cgtcgactat cgccgccacc aacagtgatt taagtacctt aaaggcaacg gttgaggatg
     2461 gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc cttaaaaagc ggctccacca
     2521 cattaacgtc attaacagcg gtgaccgatc aaaacggaat cgcgacaaca aacgtgagat
     2581 gagcgataac agggagcgtc actgtaagcg cagtcataag cgcaggtgga atgcaaacag
     2641 tagatatatc gctagtggca ggcccagcag acgcctcgca gtccatcctt aagaacaacc
     2701 agtcatcact gaaaggggac tttaccgata gtgctgagct acatcttgtt ctgcacgata
     2761 tatcaggcaa tccgatcaaa gtttctgaag ggatggaatt tgtgcaatca ggtacaaacg
     2821 tgccctatat gaaaattagc gcaattgatt acagtcaaaa tatcaatggt gattacaaag
     2881 ctactgttac aggagacgga gagggtatcg caacgctgat ccccgtattg aatggtgttc
     2941 atcaggcggg cctgagtacc acgatagaat tcattagcgc ggaaaccaga ccaatgacgg
     3001 gtacagtgtc agtgaatagt gcaaacctac cgacagcttc attcccttcg cagggattta
     3061 ccggggcgta ttatcagttg aataatgaca actttgcacc aggaaaaacg gcggctgatt
     3121 attcgttttc aagctcggcc tcctgggtag gcgttgatgc tactggtaaa gtgacattca
     3181 aaaatgacgg tgatagcaat acagtaataa tcacagcgcc cccaagatcc ggtggtgcta
     3241 tttaccaaac agttcctcct gaatcgcgtt cggtgtgact atgatcattt tcggtgagta
     3301 ttgctttgat ccaaaggctg gttttctgtg atgattgacc agcctcagag tattgatgac
     3361 accaccgttc ataacagatc ggcattacta tgcataagta ctgcctcaaa ttctcgccaa
     3421 aacacttttt aatgcctttg cgaattcatc cagacttata ttgtcaattt tttgcccaac
     3481 gacgactgac tccgaaacat cggccattgc gggccatcgc gttttgtaat cagcataaat
     3541 tgcaacaaca ggggtattat aggcacttgc catatgtaat attgaggtgt caggagttat
     3601 tgcaatataa gcatctttta tgattgctgc tgaacgttta atcgagggtg ataaggataa
     3661 acggtagaca ttattataac aatccactaa tgccctggct ttatcttccc ctttgggtcc
     3721 ataaacgatc actattggaa tatctgtttc actttgaatt tttgctatta gattttctgc
     3781 aatcgataat gaaaatgtac gttcctgcga gctaccgtct aaattgagcg caatgtaaga
     3841 tcctaacgat cgcatttcct cgcgtacctc atcgagtaca tcatcgctta atggcaattc
     3901 atatattgac ctgactgccg gaaaacccgc ctcacgcatc ataaatgccc atgtcatggg
     3961 tacaggagtc cggagattct gatccatccg ggacgcgttc ttacacaaag gggaatagca
     4021 cttcatggtt aaaccgacga cctggaaatt cgtttttgct ttcaactgac tgataaatat
     4081 catcgttttc aggttctttt tacgcatcgc ctctatacaa agatccggcg taccgtattg
     4141 cagtgttata ttcttcgcta aatcttttat ttcttttaat gttgcgtgat cctgcatagt
     4201 cattgtgact aatgttaatt tagtctgttc aagtttgagc gcattaaaga cttctaaatt
     4261 aattgtcgac gtaacaatta atagatgctt aattttatgc aattcaagcg cccgaataac
     4321 aggaaagatg gccatagcat cgccaatctg atcgggaata tgtatgacaa caaagtctgt
     4381 tttttcaata ttaaaattat aagctttata atcgtagtaa ctaaatgcaa tacgtctcaa
     4441 caatgatact aaaaacataa ctaacctcgc ctccctactg gttataatgc aatgcagtct
     4501 atcagaatca tcagggtgca attttgtgca tatgcggact tttatgtttc atatctcgaa
     4561 cctgtgggcc ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct tttatcttcc
     4621 atcgaatttt tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg
     4681 aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact
     4741 aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc
     4801 ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agtttcgctg
     4861 gtgccgtcgt cgactggtat gattttttac tctatggcat taccgccgca ctggtgttta
     4921 atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgccacct
     4981 ttggtgtcgg cttccttttc cgaccgctcg gcggtgtcat tttcggtcac tttggtgacc
     5041 ggctgggacg taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct
     5101 tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttgctggtga
     5161 cactgcgtgc cattcaggga tttgccgtcg gcggcgaatg gggaggcgca gcgttgcttt
     5221 ccgttgaaag tgcgccgaaa aataaaaaag ccttttacag tagcggtgta caagttggct
     5281 acggagtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg
     5341 acgtacagtt tttaagctgg ggctggcgca ttcctttcct gtttagcatc gtactggttc
     5401 tgggagcatt gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac
     5461 ataatcaagc ggccgcgaaa aaacgcatcc cggttatcga agctctgtta cgacatcccg
     5521 gtgctttcct gaagattatt gcgctacgac tgtgcgagtt gctgacgatg tacatcgtta
     5581 ccgcctttgc acttaattat tcaacccaga atatggggtt accgcgcgaa cttttcctta
     5641 atattggttt gctggtaggt ggattaagct gcctgacaat tccctgtttt gccaggcttg
     5701 ccgatcgttt tggtcgccgc agggtttata tcacaggtgc gttaatcgga atgttgagcg
     5761 catttccttt ctttatggcg cttgaagcac aatctatttt ctggatagtt ttcttctcca
     5821 taatgctggc aaacattgcg catgacatgg tggtgtgtgt gcaacaaccg atgtttaccg
     5881 aaatgtttgg tgccagttat cgctatagtg gcgctggagt cggttatcag gttgccagtg
     5941 tggttggtgg tggatttaca ccttttattg ccgctgcact catcacttac tttgccggga
     6001 actggcatag cgtcgccatt tatttgctgg ctggatgcct gatttccgca ataaccgctt
     6061 tgttgatgaa agacaatcaa cgcgcttgat agcctggcga agatcatccg atcttcgcct
     6121 tacacttttg tttcacattc ctgtgacata ctatcggatg tgcggtaatt gtatggaaca
     6181 ggagacacac atgaataata agggctccgg tctgacccca gctcaggcac tggataaact
     6241 cgacgcgctg tatgagcaat ctgtagttgc tttacgcaac gccattggca actatattac
     6301 aagtggcgaa ttacctgatg aaaacgcccg caaacaaggt ctttttgtct atccatcact
     6361 gaccgtaacc tgggacggta gcacaaccaa tccccctaaa acgcgcgcat ttggtcgttt
     6421 tactcacgca ggcagctaca ccaccacgat tactcgccct actctctttc gttcgtatct
     6481 caatgaacaa cttacgttgc tgtatcagga ttatggtgcg catatctcgg tgcaaccctc
     6541 gcagcatgaa atcccttatc cttatgtcat tgatggctct gaattgacac ttgatcgctc
     6601 aatgagcgct gggttaactc gctatttccc gacaacagaa ctggcgcaaa ttggcgatga
     6661 aactgcagac ggcatttatc atccaactga attctccccg ctatcgcatt ttgatgcgcg
     6721 ccgcgtcgat ttttccctcg cgcggttgcg ccattatacc ggtacgccag ttgaacattt
     6781 tcagccgttc gtcttgttta ccaactacac acgttatgtg gatgaattcg ttcgttgggg
     6841 atgcagccag atcctcgatc ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa
     6901 ctggatcacc gccgaaaccg aagcgccaga agaagccatt tccgaccttg catggaaaaa
     6961 acaccagatg cccgcgtggc atttaattac cgccgatggt cagggtatta ctctggtgaa
     7021 tattggcgtg ggaccgtcaa atgctaaaac catctgcgat catctggcag tactacgccc
     7081 ggatgtctgg ttgatgattg gtcactgtgg tggcttacgt gaaagccagg ccattggcga
     7141 ttatgtactt gcacatgctt atttacgcga tgatcacgtt cttgatgcgg ttctgccgcc
     7201 cgatattcct attccaagca ttgctgaagt gcaacgtgcg ctttatgacg ctaccaagtt
     7261 agtcagcggc aggcccggtg aggaagtcaa acagcggctg cgtacaggta ctgtggtaac
     7321 cacagatgac aggaactggg aattacgtta ctcagcttct gcacttcgtt ttaacttaag
     7381 ccgggccgtg gcaattgata tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg
     7441 cgtgccatac gggacactac tgtgtgtttc agataaaccg ttgcatggcg agattaaact
     7501 tcccggccag gctaaccgtt tttatgaagg cgctatttcc gaacacctgc aaattggcat
     7561 tcgggcaatc gatttgctgc gcgcagaagg cgaccgactg cattcacgta aattacgaac
     7621 ctttaatgag ccgccgttcc gataataagt tctgaacctt cttatcaaaa atagatgtga
     7681 aggattaagt aagacatctg gcaaatagcc cgcaataaac gcgggctgtt tgcttccttg
     7741 ggcggatacg agttttatta tcgtcttaat gatttccaca tattaaaagc aagtatgctt
     7801 tcaaaacaca attataaaaa
//
LOCUS       DAZFKM010000190         7799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFKM010000190 DAZFKM010000000
VERSION     DAZFKM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6223
                     /gene="efa1"
                     /locus_tag="ACHKAY_004692"
     CDS             <1..6223
                     /gene="efa1"
                     /locus_tag="ACHKAY_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO5703226.1"
                     /translation="DKVIFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVHTEEN
                     LLPAPSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELPPHEL
                     LTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMR
                     EGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADA
                     TLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVVS
                     SLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLA
                     LLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQG
                     NKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFG
                     HADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPEND
                     AGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSL
                     RLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTR
                     LQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGET
                     LHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTL
                     AKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTM
                     GKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLT
                     AASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVAS
                     PERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYA
                     NSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWRE
                     AVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFI
                     LRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLAFDLSKQTQSVMLKTT
                     DGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNTFHTSSGGGTVISGGGNNRYIIPR
                     DLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFELSLIYWAGNNIKVQLEDETQLNR
                     FAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQRWQAVYPEENNRPDAILDRLHDMG
                     WSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSEFRLTGSRHYTTAVTGTPGSRYII
                     TGPDTAQISPAQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLT
                     ISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMAL
                     NQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDL
                     SIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEA
                     PSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFR
                     DFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYA
                     RIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGEPFPTFHPWYTDDLSG
                     RYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILS
                     EIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHS
                     SGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRS
                     TVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIA
                     DNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6395..6745
                     /locus_tag="ACHKAY_004693"
                     /pseudo
     CDS             6395..6745
                     /locus_tag="ACHKAY_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7167..7781)
                     /gene="espF"
                     /locus_tag="ACHKAY_004694"
                     /pseudo
     CDS             complement(7167..7781)
                     /gene="espF"
                     /locus_tag="ACHKAY_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT         2293 a         1766 c         1787 g         1953 t
ORIGIN      
        1 agataaagtt atatttaaag ggcaaacact ggatcgtctt tatcatgaag gatatgtatt
       61 ttctgatatc aataccttgt ctcgttatac actacacgga ctaggaataa ctggtgtaca
      121 tactgaagaa aacctgctac cagctccttc atcgtcctta attaatatat tgaaagaaca
      181 ttataatgaa gatgaaatta gtgcgaaatt accactagca tatgattaca ttttaaataa
      241 aaaagaatca agctctattc ctgttgaaat tttgaacaaa ctttcagagt taccaccaca
      301 tgaactactc acacctgttc ttggccagag tgttaatcct ctgggcatgg gctactcatc
      361 cgataatgga aaaatcacag agcaagtaat agtcagtgga gctgatggat ttgataatcc
      421 catatctgga cttatatata cctatcttga agatctatat aacatccatg taaggatgcg
      481 agaaggtaca ctaaattcac agaatcttcg tcagcttctg gaaaactctg tttcttcatg
      541 ctttttgact gagcaaagta ttaataaatt acttagtgag gcagaaaaaa ggccttatca
      601 gtctttaaca gaaatacatc agcatctcac aggattacca actattgccg atgcaaccct
      661 ttcattactt tctgttggat tacctggtac gggaaaacta ttgcgcaggg agcaggacta
      721 tgggcgtcca ccagttacag caattcagga ttccacattt gtactccctt ataatttcaa
      781 aggtattggt tttaacgata acattatatc ctctgcacct gtagtctcct cattacattt
      841 tatcgctgaa catgcgaaat atactttgtt gtcatggcct gagttttatc gtcatcatgc
      901 acagcgatgg ttcgaaatgg ctaaaggata tggaagccag aatattgatt ttcaccctca
      961 gtctctattg gtaacccaag aaggacgctg tatgggatta gccttacttt atttacagac
     1021 tgaagatact gctcattata gcattctcca ggaaaaccta atgactgtga gtgcacttca
     1081 tcagaccagt aatcgcgata agttgccact gtccaaagat gataattcct taatgacaag
     1141 aacttatagt ctgattgaaa tgctacagta tcagggaaac aaatatatta ccaacgaatc
     1201 gctactacat aagaccgcat ggaaccaaga aagaataact ttattattca atgaaaaagg
     1261 agttaagcga gccctgataa gcacgcctaa tcatactctg gttctgcaac aactggagga
     1321 tatttaccgg ctcactgatc caaattttgg gcatgcagat ttcctttcac ctatagatgc
     1381 tctgaagttt attgaggcta tgatacaatt aactccaaca cttcaggaat attatggcct
     1441 attaaacaaa gacattaata aacatataca agtgcattat gccgaatcag atatggtctg
     1501 gaataagctt ctgccagaaa atgatgctgg actgagcacc agaattcagc acaccaccac
     1561 cgaccgtctg gcgaatctgg ctgaaccagt cgctgttgca ggtatctccc tgccagtaaa
     1621 aacactttat gatatcggag ccacccttga cggtcggcgc atcacctctc ctccaacatc
     1681 ggagcaaatc ccttctctgc gtctcaacgg tgatgttctg aatgattatc tgtcccgcac
     1741 agttctgact ccagaacagg ctgataacat aagaaaaata ctgcacactc agggaatacg
     1801 cagcggtacc cgtcccatag atccggagat gattcgtggg acgcaggatg acctagtttc
     1861 gtcacagact cgtctgcaaa ggcaagcaac acgggttaaa cagcaactcg ccggcgtact
     1921 tgatactctg caacagcact tccagaacat tccacgttca tccggtcgtc atctttctgt
     1981 agagaatatt gagctggctg atatcggaag tgggcgtttc aaccttcaaa ttcgagatgg
     2041 agaaacattg cataccactt ctgtggaagt accggaagtt gtgtcccgtt ttcagaaact
     2101 ttctaccatg ctttcagccc tgcctgccag tggaatcatg gatttcgacc tcggcatgag
     2161 tgtggtcgga gtcgtccagt atgcccgcct gctacagcag gggcacgaag acagtaccct
     2221 agccaagata aatctagcta tggatatcaa gcaactttcc gaagcaactc tcggcagcat
     2281 gattcagatt gccgggaata agtttctcaa tacagaagga atccaggggt tcagactgga
     2341 aagcgccgtc gctgaaggta tgcgctcagt agcaacccgt accggaggca caatggggaa
     2401 agccctttct gccagtgccc gtgttcttga actgcctgta ctggaaacag ttctggggac
     2461 atggaacctg tacaacagcg tcattcagct ccagcaagct acatcttatt ctgagacaat
     2521 ggctgcccgg gtacagattg cgtttgattc catttctctg ggattaactg ccgcttcggt
     2581 agctttcccg ccactaatta ttgccactgg ccccattgcg gctattggta tgggagcttc
     2641 cagtattgca cgtaatgtgg cacggaaaga agaacggcat acacaatggc tggaatataa
     2701 aaaattcctg actgatggca gtaaacacat tgttgtggcc tctccggaaa gaggtctgct
     2761 ggatttctcc ggaaacaaag tttttggaaa aatggtgctg gatctgcgtc agtctcctcc
     2821 tctcttgcat ggagaaagct cttttaacgc tgaccgcaaa atcggtcatc gtccggatct
     2881 gggggactgg caaattcgtg agaaggtggg gtatgccaac agtatcagtc cctactcttc
     2941 cctggcgcac ggttacgcca acagtaaatg gccacaaaca ataccgaaaa ttccctcggg
     3001 agaatatgac accataattc tgggctacgg tcaccagtat caggccaata cggaaataga
     3061 atatctgtct aactggattg tatggcggga agccgtacca gacagtactt cccgccacaa
     3121 acgtcctcct ctggaggttc ttaatagtca gtgtactgtg atagctggag agcgtaaaac
     3181 cacagtactt cccctgagag tgctcagcga tctgacaccg gaatgcacag aacaggctat
     3241 atcgttaaaa gattataaat tcatactgag agggggaagc ggtgggctgg ctgttcaggt
     3301 cggtggcgcg ggatattatg atattgatgc aaatcctgtg gcaaaagaaa atacgctctc
     3361 ttttcgcggg ctaccggaag agtttccgct cgcctttgat ttatcaaaac aaacacagtc
     3421 ggtcatgctg aaaacaacgg acggtgaggt gccggtaatg accatcaccc agaagggaat
     3481 aaacaccctg gtcggtacaa cggccggcga aaactggcta acgggtaacg ataaggacaa
     3541 taccttccat acgagttctg gtggcggtac tgtcatctct ggtggaggga ataaccgcta
     3601 tattatcccc cgcgatttaa aaactccgct gacactgacg ctgtctaata gctcggtctc
     3661 tcacgagatc cttctgccag aaacaactct gacagaatta aaacctgtcg cctttgagct
     3721 gagtctgatt tactgggccg ggaacaatat aaaggttcag ctggaggatg agacacaact
     3781 gaaccgtttt gccggaaact tcagggggca taccagtgat ggcattactc tggaggcggt
     3841 ttcccaggaa aatggtattc aactgggggt ttcatcatgt gatgttcaac gctggcaggc
     3901 tgtttatccg gaagaaaaca acagaccaga cgccatactg gacaggctgc atgatatggg
     3961 atggagcctg gcccccgagg tcaggttccg gggaggagaa acatctgcca gctatgaccc
     4021 cctgaaccgt cagctcgttt accggcttca ggagcgttac tcggaattca ggctgaccgg
     4081 cagtcgccat tacaccacgg ctgtaaccgg aacccccgga agccggtaca ttattacggg
     4141 gccggataca gcacagatat caccggcaca aatcatactg gccggtgata atgaccatcc
     4201 ggaaacgatt gatttgcttg aggccagtcc tgttctggtt gaagggaaaa aagacaaaaa
     4261 cagcgtgata ttaacgattg ctacgattca gtattccctt caactgacaa tatccgggat
     4321 cgacgaatcg ctgcccggga caacccgtgt ggcaattcag cctcaggata cccgtttact
     4381 gggtgacgta ctccggatct taccagataa tggtaactgg gtggggattt tccggagtgg
     4441 tcatacacca acggtaaacc ggctggaaaa tttgatggca ctgaatcagg taatgacgtt
     4501 cctgccccgg gtatccggaa gtgcagagca ggtattatgc ctcgaaaacc taggtggcgt
     4561 aaggaaaaaa gtggaggggg agttactgtc agggaagctg aaaggtgcgt ggaaagccga
     4621 aggtgaacct actgttccgg taaatatctc agatctaagt atcccaccct attcacgtct
     4681 gtatctgatt tttgaaggga aaaataatgt gttgctacgc agtaaagtac atgcagctcc
     4741 gttgaaaata acatccgcag gagagatgca gttatctgaa aggcagtggc aacagcagga
     4801 acatattatt gtcaagcccg acaacgaagc cccctcatta atactcagtg aatttcgtcg
     4861 tttcactatt tcatcggata aaaaattttc tttaaaactg atgtgccatc aggggatggt
     4921 ccgcatcgac cgcagatcat tatcggtcag attgttctat ctgcgtgaac agccaggtat
     4981 cggcagttta cgtctgacgt tcagagattt tttcacagaa gtgatggata caacggacag
     5041 ggaaattctg gagaaagagc tgagaccaat tctgatagga gatacacacc gctttatcaa
     5101 cgctgcatac aaaaatcatc tgaatatcca gttaggagat ggcgttctga atctggcaga
     5161 cattgttgcg gaatacgccc gtattcaaaa ggaagaaaca tcaaaaatat tgtatcaata
     5221 tcaaggtgcc atgaaaaaaa aaacagatgg accatctgtg gtagaagatg ccattatgac
     5281 cactactgtc acaacagatt caggtgaacc attccctacc ttccacccgt ggtatacaga
     5341 tgatttatca gggcgttata agagcgtacc tatggcaaga aaagcagata ctttgtatca
     5401 cctgacacca aaaggtgatc tacagataat atatcaggta gctacaaaaa tggtgaatca
     5461 ggcgatgatt gtatccctgc caaactaccg acacgagtgg gaaaaatata atttaagcat
     5521 cttatccgaa atccctcaga acaataatac tgttgtacat tcaatcctca gggttaatgg
     5581 ccccacaatg caggtgcgca caattgacta cagaggaacg gatgaaaaca atcccatagt
     5641 atctttttca gatacaacct tcatcaatgg tgaacagatg ttgagttatg actcgcattc
     5701 atcagggcga gtctattcca gagaagaata tatgatgtgg gaattgcagc aacgggtatc
     5761 agaagcttcc agtgcccgga cacaggatta ctggctgatg gatgcagcgg taagaaacgg
     5821 agaatggaag atcacaccag aattattacg tcacacaccg ggatatatcc ggagtacggt
     5881 atcgaaatgg tccagaggat ggctgaaaac cggcacaata ctccagactc cagaagacag
     5941 aaatacggat gtatacctga ctaccataca gaacaatgta tttagtcgtc aggggggcgg
     6001 ctaccaagtg tattatcgga ttgatggaat ggctggtgcg gatatagcgg ataatgcacc
     6061 aggggaaacc cgctgcaccc tcaggcccgg aacatgtttt gaagtgacaa gtgtggatga
     6121 aaggcattat gagtggaata tcatttatgt cacgctgaaa acctgtggct ggagccgaaa
     6181 tggccaaagc aaaacgccga atggtgacaa cctttttaac taattaaaat ctctgttaaa
     6241 gatgtataac ccggcatcta tgtgctcacc agcatataga tgcaatgtga ttcccctgat
     6301 ggtcgtatca gagatggtat gcgagcctgt acatagattt gtgtaattgc ctgattttga
     6361 tatgttcaat ccaacatcaa aagcaggtta atttatggac gaaaaacagt tgcaggctct
     6421 ggctaacgaa caggccaaaa atctcaaaac ccctgaagat ctcagccagt tcgatcgcct
     6481 gctgaagaaa atcagcgttg aggcagctct caacgctgaa atgtcccacc atctgggcta
     6541 cgataaaaac cagcctaaac cgggtgccaa ctcccgcaat ggctattcca caaagaccgt
     6601 tatcaccggt gatggccccc tggaactacg cactccgcgc gatcgtgatg gctcttttga
     6661 accgtaactt gtgaagaaaa accagacccg gatcaccggg atggataacc agatcctgtc
     6721 gttgtacgcc aaaggcctga caaccgtaag cgtacagcct gaaccgtctg gtcagaatct
     6781 gacgaattag agtaagctgg acatccccag atatattatg gctgccagcg tcatatcggc
     6841 ataaatagag atatccctta acgctaacca gaaaaaaata cctataagag cctgtacata
     6901 gagttgtgtc gttgcctgat gttgatatgt caaatccaac atcaaatagc ggtaaattta
     6961 tggataaaaa attacaggct ctggttaacg aaacaccccc ctcttgctct attcccctct
     7021 ttcatcattt attacaatat ttatttcaat aaaaaccgat attaccaatc acatttatca
     7081 caatgcaact taatatcggt ttttatcttt taactctcta aacaatcaac agtaattaat
     7141 aattttaaat ctacttttta tgcctttttc gacagttcat aggcagctaa atgatctttt
     7201 aatgcctgag caataggcgg taatggccgg gatgcctcgg atgcctgtcc acttgtcggt
     7261 ggcggcggtg ccgggcggct tggcttaaga cctgaagtat caagactttt cgatttttca
     7321 taggcagcca aatgatcttt tagcgcctga gcaatgggcg gtaaagaccg ggatgaccca
     7381 gataccaacc cgcttgtcgg cggcggcggt gccgggcggc ttggcttaag gcttgaagta
     7441 tcaagacttt tcgacttttc ataggcagct aaatgatctt ttaatgcctg agcaatgggc
     7501 ggtaaagggc gggatgcccc ggaagcctgt ccacttgtcg gcggcggtgg ggccggacga
     7561 gagggagtaa atgaagtcgc cgggctactc acattaaaag tcgttctcgc attgatattc
     7621 gacgtaccgg gagaaaaagg agattgagcc ctaatcggat tcagacgaac agcctgaggg
     7681 gacactgaaa agccagataa ccccgcagag cttacccggc ttgccgcact agtaatatgc
     7741 cgtcctagtg tagaaacagc ttgactaatt ccattaagca taattcatgc ctttttata
//
LOCUS       DAZFKM010000191         7604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFKM010000191 DAZFKM010000000
VERSION     DAZFKM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..1751
                     /locus_tag="ACHKAY_004695"
     CDS             66..1751
                     /locus_tag="ACHKAY_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO5703227.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
     gene            1789..4161
                     /locus_tag="ACHKAY_004696"
     CDS             1789..4161
                     /locus_tag="ACHKAY_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5703228.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            4218..7001
                     /locus_tag="ACHKAY_004697"
     CDS             4218..7001
                     /locus_tag="ACHKAY_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO5703229.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            7363..>7604
                     /locus_tag="ACHKAY_004698"
     CDS             7363..>7604
                     /locus_tag="ACHKAY_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5703230.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         2214 a         1680 c         1708 g         2002 t
ORIGIN      
        1 aaataaattt catcagtaaa tcattgccat agataatcat tggatggcac aatcaatttt
       61 cgctaatgat aaccattccc attacgtttt gtatgttgat agcaaagtat ctctgtctgc
      121 tgaagccctt ttggctgcgt aagaataata aaacttcagt gttgttaatc attattatcc
      181 tcgccatgat ccttggcgtg gttaaaattc aggtctggtt aaacgactgg aataatgatt
      241 ttttcaatgc gctaagccag aaagaaaccg ataagctttg gcagcttgtc ctttggttcc
      301 ctgccctgct ggggatcttt gtattaatat ccgtaaacaa aacatggtta attaaactac
      361 tgactattcg ctggcgcgaa tggttaacag attactacct taatcggtgg tttgcagata
      421 aaaactacta cttcacgcag atctacggcg agcataaaaa tacagataac cctgaccaac
      481 gtatcgctga agacattctc ttactgatca gcaaaacgct cagcctttca tttggcttca
      541 tccagtcact tagcatgctg atcaccttta ccgttattct ttggcaaagc gcgggtacgc
      601 tctcattcac tgttggtgga acagaatgga atatccaggg gtatatggtc tataccgttg
      661 tgctcattgt gatcggtgga actttattta cccataaggt tggtaaacgt attcgtccgc
      721 ttaatgtgga aaaacaacgt agcgaagcga catttcgaac aaatcttgtg cagcataata
      781 agcaggcaga actgattgcg ctaagcaatg ctgagtctct acaacgccag gagttgagcg
      841 acaattttca taccattaaa gagaactggc atcgtttaat gaatcgtcag cggtggcttg
      901 attactggca gaatatttat tcgcgctcgc ttagcgttct tccctacttt ctgttattgc
      961 cgcagtttat tagcgggcag atcaatctgg gcggactgat gaaatcgcgc caggcattta
     1021 tgctggtatc gaacaattta agctggttta tttataaata tgacgaactt gctgaactgg
     1081 ctgcggttat cgatcgtttg tatgagttcc atcaactcac cgaacagcgc cctacgaata
     1141 aacctaaaaa ttgccaacat gcggtacaag tggctgacgc gagtattcgt actcctgata
     1201 ataagatcat attagagaac ctgaactttc atgtttcgcc aggcaaatgg ctattactga
     1261 aaggttactc tggtgcggga aaaaccacac tgcttaaaac attatctcac tgctggccgt
     1321 ggtttaaagg tgatatttct tctcctgctg acagttggta tgtgtcacaa acaccgttaa
     1381 tcaaaaccgg cttactgaaa gagattattt gtaaagcact tcccctgccc gtagacgata
     1441 aatcgttgag cgaagtactg catcaggttg gtctggggaa attggctgcg cgtattcatg
     1501 accacgatcg ctggggagat attctttcca gcggcgaaaa acaacgtatc gccctggcac
     1561 gattaatttt acgacgtccg aaatggatat ttcttgacga aactacctct catcttgagg
     1621 aacaagaggc tatccgctta ctgcgtttag tgcgtgaaaa actacccaca agcggcgtca
     1681 ttatggttac acatcaatcc ggtgtctgga acctggcaga tgatatttgt gacattagcg
     1741 cggttttata aaaacaataa aagccctgct gtacggagat ataaataaat gaagcgagtt
     1801 cttattcctg gcgtcatttt atgtggcgct gatgtggcgc aggccgtcga tgacaaaaac
     1861 atgtacatgc atgtttttga agagatgacg gtctatgctc ctgtccctgt acccgtaaac
     1921 ggcaacacgc attacaccag tgaaagcatc gagcgtttac cgaccgggaa tggcaatatc
     1981 agcgatctgc tgagaaccaa ccctgcggta cgcatggatt caacgcaaag tacctcgttg
     2041 aaccagggag atattcgccc ggagaaaatc tctattcacg gtgcgtcgcc ctatcagaat
     2101 gcctatttga ttgacggtat tagtgccact aataacttga acccagcgaa tgagtccgat
     2161 gccagtagtg caaccaatat tagcgggatg tcacaggggt attatcttga tgtcagctta
     2221 ctggacaatg tgacgcttta tgacagtgtt gtgccggttg aatttggtcg cttcaatggc
     2281 ggggtaattg atgcaaagat caaacgcttc aacgctgatg atagcaaggt gaaattgggt
     2341 tatcgcacta cgcgttcgga ctggttaaca tcgcatatcg atgagaataa caagagcgca
     2401 tttaatcaag gctcttcagg aagtacttat tactccccag attttaaaaa gaacttttat
     2461 accttgtcgt ttaatcagga actcgctgat aacttcggcg ttaccgccgg tttatcgcgc
     2521 cgccagtctg atatcacccg cgcggattat gtttcgaatg acggcattgt cgccggtcgg
     2581 gcacagtata aaaacgttat cgatactgca ttgagcaaat ttacctggtt tgccagcgac
     2641 cgctttaccc acgatttaac cttaaaatat accggctcca gccgtgatta taataccagc
     2701 accttcccgc agtctgatcg cgaaatgggt aataaatcct acggtctggc atgggatatg
     2761 gatacgcagc tcgcatgggc caaactgcgt accaccgttg gttgggatca tattagtgat
     2821 tatacccgtc acgatcatga catctggtac accgaacttt catgtacata tggtgatatt
     2881 acagggcgtt gtacccgtgg cggattagga cacatttccc aggctgtaga taattacacc
     2941 ttcaaaacac gcctggactg gcaaaaattc gccgtgggtg atgtttcgca tcaaccctac
     3001 ttcggcgcgg aatacatcta ttccgatgca tggactgaac gccataacca gtctgaatcc
     3061 tatgtgatta atgctgcagg aaagaaaact aaccatacca tttaccataa aggtaaaggc
     3121 agcctgggaa ttgacaacta cacgctgtat atggcggatc acattagctg gcggaatgtg
     3181 tcgttaatgc ccggtgtgcg ttatgactat gacaactatc tgtcaaacca caatatctcc
     3241 ccgcgcttta tgacggaatg ggatattttt gctgatcaaa cctcaatgat tacagcaggt
     3301 tataaccgtt actatggcgg gaatattctt gatatgggat tacgtgatat ccgcaatagc
     3361 tggacggaat cggtatcagg caataaaacc ctgacgcgtt atcaggattt gaaaacgcct
     3421 tataacgatg aactggcaat gggattgcag cagaaaatcg gtaagaacgt tattgcgcgc
     3481 gcaaactatg tttaccgtga agcgcatgat caaatcagca aaagcagtcg taccgacagc
     3541 gcgactaaaa ccaccattac tgaatataac aacgacggca aaacaaaaac gcattcgttc
     3601 aacctcagtt ttgaactggc cgaacccctg catatcagcc aggtagatat taacccgcaa
     3661 attgtcttta gctatatcaa gagcaagggc aacttgtcgt taaacaatgg ttatgaggag
     3721 agcaataccg gtgataacca ggtggtttat aacggtaatc tggtttctta cgatagcgtt
     3781 ccagtggcag attttaataa cccattaaag atctccttaa acatggattt cacgcatcaa
     3841 ccgagcgggt tagtgtgggc gaatacgctg gcctggcaag aagcgcgtaa agctcgcatt
     3901 atcctgggta agacaaatgc gcaatacatc agcgaatatt cagattacaa gcagtatgtt
     3961 gacgaaaaac tggatagcag cctgacctgg gacacccgct tgtcctggac gccacaattt
     4021 ctgaaacaac aaaacctgac gatcagtgcc gatattctca atgtattgga tagcaaaacc
     4081 gctgttgata caacgaatac cggtgtggcg acctacgcca gtggccgtac tttctggctt
     4141 gatgtcagca tgaaatttta atagttcgga ttttacttaa ccgggtaaat tcgcccggtt
     4201 ttcgcatgga gataattatg agaaacctct gtttcttact gacgttagtg gcaactctgt
     4261 tgctccccgg gcggctgatt gccgccgcct taccgcagga tgaaaagtta attaccgggc
     4321 aactgggcaa tggcttgcga tatatgattt atccgcatgc tcatccaaaa gatcaggtaa
     4381 atttatggct gcaaattcat accggttcat tgcaggaaga agacaatgag cgcggcgtgg
     4441 ctcattttgt agaacatatg atgtttaacg gcacaaaaac atggccgggt aataaagtca
     4501 tcgaaacatt tgagtcaatg ggcctgcgtt ttggtcgcga tgttaatgcc tataccagct
     4561 atgacgaaac ggtgtatcag gtgagtttgc cgactacgca gaaacaaaat ctgcaacaag
     4621 tgatggcaat cttcagtgaa tggagtaatg ccgcaacctt tgaaaaactc gaagtagacg
     4681 ctgaacgtgg cgtaattact gaggaatggc gtgcccatca ggatgcgaaa tggcgcacct
     4741 ctcaggcgcg ccgccctttc ctgctggcta atacccgtaa tttagaccgt gaacctatcg
     4801 gcctgatgga tactgtcgcc acggtcacac cggcacaatt gcgccaattt tatcaacgct
     4861 ggtatcaacc aaataatatg acctttatcg tggttggcga tatcgacagt aaagaagcgc
     4921 tggcgctgat aaaggataat ttaagtaagc ttccggctaa caaagcagct gaaaatcgcg
     4981 tctggccgac aaaagccgaa aaccacctgc gctttaatat cattaatgat aaagaaaacc
     5041 gggtgaacgg catcgcactc tattatcgcc tgccaatggt acaagtgcac gatgagcaaa
     5101 gctttatcga acaagctgaa tggagcatgt tagttcagct gttcaatcaa cgtctgcagg
     5161 aacgcataca gtcgggcgag ttgaagacta tttctggcgg cactgcgcgc agcgttaaaa
     5221 ttgcacccga ttatcagtcg ctgtttttcc gtgtaaatgc acgagacgat aatatgcagg
     5281 atgctgcgaa tgcattaatg gcagagttgg caactattga tcagcatggt ttttctgctg
     5341 aagaactcga tgatgttaaa tctacccgcc tcacctggct gaaaaatgcg gttgatcagc
     5401 aagctgaacg tgatttacgt atgctgacca gtcgcctggc atccagctca ttaaataata
     5461 cgccgttctt gtcgccggaa gagacatatc aactttcgaa acgtctgtgg cagcaaatta
     5521 ccgtgcaaag tctggcggaa aaatggcagc agttaagaaa gaaccaggac gcattttggg
     5581 agcaaatggt aaacaatgag cttgccgcca aaaaagcatt gtctcctgcg gctatcctgg
     5641 cgctggaaaa agagtacgcc aacaaaaagc tggcggctta catcttccca ggcagaaatt
     5701 tatcgttaac agtagacgct gacccacagg cggaaattag cagcaaagaa acgctggctg
     5761 agaatctgac atcattaaca ctttccaatg gtgccagggt tattctggca aaatccgcgg
     5821 gtgaagagca aaagctacaa attactgccg tatcgaataa aggcgattta agtttccctg
     5881 cgcagcaaaa atcacttatc gcgctggcaa ataaagcagt aagcggaagc ggcgttggtg
     5941 agctctcctc ttccagcctg aaacgctgga gtgcggaaaa ttcggtaacc atgagcagta
     6001 aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg gactaataac cctgaacctg
     6061 gttttcagtt gattaaccag cgaatcaccc acagcacgat taacgacaat atttgggcat
     6121 cgctacaaaa tgctcaaatt caggcgttga aaacgctcga ccagcgtcca gcggagaaat
     6181 tcgcccagca gatgtatgag acgcgctatg ctgatgaccg cacgaaatta ctgcaagaaa
     6241 atcagattgt acagtttact gccgcagatg cgctggctgc cgatcgccag ttgttttcat
     6301 ctccagcgga tatcacgttt gtcattgtcg gtaatgtctc agaagacaaa ctcgtggcgt
     6361 taattacgcg ttacttagga tcaatcaaac actctgattc gccattagcc gcaggtaaac
     6421 cattaactcg cgcgacggac aacgcatcgg ttactgtaaa agaacaaaat gaacctgtgg
     6481 cacaggtttc acagtggaag cgttatgatt cccggacacc tgttaatctg gcgacgcgta
     6541 tggcgctcga tgcttttaac gtcgcactgg caaaagatct acgtgttaat attcgtgaac
     6601 aggcatctgg agcatacagc gtttcttctc gcctctcggt tgatcctcag gccaaagata
     6661 tcagtcattt gctggctttt acttgtcaac cagaacgaca tgatgaactg ttaacgttag
     6721 cgaatgaagt gatggttaag cgtctggcta aagggatcag tgagcaagaa ctgaatgaat
     6781 accagcaaaa cgttcagcgc agcctcgata tccaacagcg tagcgttcaa caattagcga
     6841 acactattgt aaatagtctt attcaatatg acgatcctgc agcatggact gagcaggagc
     6901 aattgttgaa acaaatgacg gtagagaatg ttaacactgc cgttaaacaa tatctttctc
     6961 atccggtaaa tacttatacc ggagtattat tgccaaaata ataacagccc cgtcaacaca
     7021 tcgttggcgg ggattttagc aatattcgct atttttatgt aataatttta taaatgcgtt
     7081 caaaataata atcaagtact aatagtgata ttttaaggtc tgatttttac gtgataattc
     7141 aggagacaca gaatgcgcat aaaaataaca gcataaaaca ccttaccacc acccaagaat
     7201 ttcatattgc attgtttttc aatgaaaaaa tattattcgc gtaatatctc acgataaata
     7261 acattaggat tttgttattt aaacacgagt cctttgcact tgcttacttt atcgataaat
     7321 cctacttttt taatgcgatc caatcatttt aaggagttta aaatggataa gaagcaagta
     7381 acggatttaa ggtcggaact actcgattca cgttttggtg cgaagtctat ttccactatc
     7441 gcagaatcaa aacgttttcc gctgcacgaa atgcgcgacg atgtcgcatt ccagattatc
     7501 aatgacgaat tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc
     7561 tgggacgacg aaaatgtcca caaattgatg gatttatcca ttaa
//
LOCUS       DAZFKM010000192         7378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFKM010000192 DAZFKM010000000
VERSION     DAZFKM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..843)
                     /locus_tag="ACHKAY_004699"
                     /pseudo
     CDS             complement(20..843)
                     /locus_tag="ACHKAY_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(897..2615)
                     /gene="proS"
                     /locus_tag="ACHKAY_004700"
     CDS             complement(897..2615)
                     /gene="proS"
                     /locus_tag="ACHKAY_004700"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO5703231.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2727..3434)
                     /gene="tsaA"
                     /locus_tag="ACHKAY_004701"
     CDS             complement(2727..3434)
                     /gene="tsaA"
                     /locus_tag="ACHKAY_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO5703232.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3431..3835)
                     /gene="rcsF"
                     /locus_tag="ACHKAY_004702"
     CDS             complement(3431..3835)
                     /gene="rcsF"
                     /locus_tag="ACHKAY_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO5703233.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3953..4768)
                     /gene="metQ"
                     /locus_tag="ACHKAY_004703"
     CDS             complement(3953..4768)
                     /gene="metQ"
                     /locus_tag="ACHKAY_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO5703234.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4808..5461)
                     /locus_tag="ACHKAY_004704"
     CDS             complement(4808..5461)
                     /locus_tag="ACHKAY_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO5703235.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5454..6485)
                     /gene="metN"
                     /locus_tag="ACHKAY_004705"
     CDS             complement(5454..6485)
                     /gene="metN"
                     /locus_tag="ACHKAY_004705"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO5703236.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6673..7248
                     /gene="gmhB"
                     /locus_tag="ACHKAY_004706"
     CDS             6673..7248
                     /gene="gmhB"
                     /locus_tag="ACHKAY_004706"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO5703237.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1807 a         1915 c         1876 g         1780 t
ORIGIN      
        1 gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc ttgatataaa
       61 tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac gcagagacat
      121 caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc gccagtgtaa
      181 gtggatgccc ggctttggca aaggcatccg tgacgaattc cgaacaaaac cacgactttt
      241 tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg cactttggcg
      301 aaaatcctcg gagaacggat tcagtgagca catctggcga gtcaccataa agggaataaa
      361 ttcgacaatg ccgcgatagt tataaccgct atctttgatt ttattggcaa acgcggtgat
      421 atctgtggct tgttgcgggg taagatccgg gactcgtaag acgaaaagct tatcactatg
      481 cttcatcgct tttttaaggg aaacaatctg gacgccagcg cctgtcgctt ctgcaacgtt
      541 attatcaccc agaaagattg caacgtgact cacagaggaa gtgctgaaga cgcggattcc
      601 aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg ccgggtttga gatcaggtgc
      661 cgttatttct tttattgatt gttcggtgaa agagctttga tgctggaatt tgacagccca
      721 tgtttttgcc tcggcatcta ctgctgtggc tgatgggtct ggctggctga tatcaacggt
      781 acaagccgaa agtaaaagaa agctggggag aaacaggcgg cagtacgcct ttggtttatc
      841 cattttatac aatccatgta aaaaaagggc cctgaaattc aggacccttt ctggcatcag
      901 cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa ctgtttctcg
      961 ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc gcccagcaca
     1021 atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg acgctctttg
     1081 cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag tttctcagca
     1141 agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac ctggaacggc
     1201 gcgatagcgt caggccatac gatgcctcgt tcgtcgtagt tctgctcaat cgccgcagct
     1261 accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt acggccatct
     1321 tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg gaagatgtga
     1381 ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct tggatcgcca
     1441 gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc ccagttgatg
     1501 ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat cgccgcaacg
     1561 gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc cggaccggct
     1621 ttaaccacgg cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc aacctgcggc
     1681 agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag cgcaaccagc
     1741 gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt ctcaatcggc
     1801 agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc aaccagcgtc
     1861 atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc cagttcaatg
     1921 ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct ctgcgccagc
     1981 acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcttg tacggcgcgg
     2041 aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc atcgtaggtt
     2101 tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag gaattcgcgg
     2161 gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg gatctgatag
     2221 aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc agtgataact
     2281 tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa acgcagcagt
     2341 tccggaccgt actgttccca acgaccactc tcttgccaca aatcagctgg ctgaaccacc
     2401 ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat gttttcgact
     2461 tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc cagcttgcgg
     2521 atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc aggtgtctcc
     2581 ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca gttggaaggt
     2641 agaacaggct caaggcgagc ctgggacaaa aaaaagtgat ttagtttacc agtgcaaaag
     2701 aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac ttcaaaacct
     2761 gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata ggttttgccc
     2821 gtttcctcac ctttacgata ggccgggcgt gggtcctgcg ccagtacttc gcggataaac
     2881 agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac ctctgcggta
     2941 aaactcaccg ccatctctgc cgctggcgca ctttgcgcat agctggcact ggcatcggga
     3001 agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc atcgaccaga
     3061 tccagactgc cgagcttcag aatcacgctg tctttatggc aaacaacctc tttcagctct
     3121 accagcgaca taccaattgg gttagggcgg aaagtagagc gtgttgcgaa aacccccatt
     3181 ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc ttccatcgtt
     3241 tgatgaaaga cgaaaaggat ccataaatgg ctgaacgctt ccaggccgcg aacggcgtcg
     3301 gcctggttgt agggagcaat gagatgcagt tcgccgttgg cgctttttac cagacctggc
     3361 tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat ttgctcgaac
     3421 tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca gcctgacgat
     3481 agcagcctgg cgtaccgctg gtgacttcgc agctatgcag taataccgca ttggccttca
     3541 ttttcgaggc gttgatttgc atccgcttac gcgcggttgg aatgctcggc ggagagtcct
     3601 gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac ggtttgccga
     3661 ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct ttcggttttg
     3721 caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg gatctgctta
     3781 acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca cgcatagtat
     3841 ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa atgacaagac
     3901 gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa aattaccagc
     3961 ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca tcagactgat
     4021 aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc gttacgatca
     4081 ggtttacgta cggggagtct ttatcttcga caaagatacc gtctttcgct ggagtcaggc
     4141 caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg tcgtccagag
     4201 atcgaggcag ctgcggtgct tccagctcaa caattttcag attttttggg ttctcaacaa
     4261 catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag cccacttttt
     4321 gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca acctgcgaac
     4381 catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc ggataaacaa
     4441 aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga tcaaggtacg
     4501 gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg tttggcagaa
     4561 catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc gcaactttct
     4621 gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta atgtggtttg
     4681 gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg gctcccactg
     4741 ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat ttatgttgtg
     4801 ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcgaac tgaattaaat
     4861 aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgtta tagccgatgt
     4921 agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca cccatcgcgg
     4981 aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc ggcaatgctt
     5041 ccggtaacag cactttacgg acgatctgca tcggcgtggc acccattgca cgggaagctt
     5101 caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca ataaacggtg
     5161 ctgcaccaac ggttaacgga acaatcgctg cctgcaaacc aatcgatgta ccgacaataa
     5221 cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa cggaaaatgt
     5281 tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc cccgggcgcg
     5341 taacataaag caggacgccg accggcagac caatcacaaa gccaaaaaat ccggatacga
     5401 aggtcattgc cagcgtttcc catacgccac gaaccagcaa ccacatcatc ggctcagaca
     5461 taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc ggcttgcgta
     5521 tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga acttaacgcc accggcgtaa
     5581 tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt ttcagaaagc
     5641 agcggggcat cgaccgattg accggtaaac tccaggcgca acatcggcac gcagtcagta
     5701 aatggctccg cttgcagacg ttcctggtaa tcttccggga tatccagatg cagggtcgac
     5761 tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact taccgtgtcc
     5821 tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg cttcacaacg
     5881 tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat gtctttcagc
     5941 agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc atcacacagc
     6001 aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt ctgcccaccg
     6061 gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga cagcaattcc
     6121 gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg cagagccacg
     6181 ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat accaatctgg
     6241 cggcgagctt tggtcaactc ggattctgac agcgtggtca gttcctggcc atcgaccagc
     6301 acgctaccct cggttgggcg ctccagcagg tttacacaac gtataagcgt actcttaccc
     6361 gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag gctgacgttg
     6421 ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt cgaaagtttt
     6481 atcattgatt atttattatc gtcattaagt tagtcgtggc atctcgaatg cctgaaacgg
     6541 gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa tcaggtttat
     6601 gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg ccttattaag
     6661 gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga tggcaccatt
     6721 aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga cggtgttatt
     6781 gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac caaccagtct
     6841 ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga gtggatggac
     6901 tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc gcatcatccg
     6961 cagggtagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca tccggggatg
     7021 cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat ggtgggcgat
     7081 aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt gctggtgcgt
     7141 acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt aaatagcctg
     7201 gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cacaatgatt aaaagatgag
     7261 cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc tataatgcgc
     7321 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagag
//
LOCUS       DAZFKM010000193         7328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFKM010000193 DAZFKM010000000
VERSION     DAZFKM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..339)
                     /locus_tag="ACHKAY_004707"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHKAY_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(655..1299)
                     /locus_tag="ACHKAY_004708"
     CDS             complement(655..1299)
                     /locus_tag="ACHKAY_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703238.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="ACHKAY_004709"
     CDS             complement(1320..1589)
                     /locus_tag="ACHKAY_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5703239.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="ACHKAY_004710"
     CDS             complement(1668..2306)
                     /locus_tag="ACHKAY_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO5703240.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..3522)
                     /locus_tag="ACHKAY_004711"
                     /pseudo
     CDS             complement(2291..3522)
                     /locus_tag="ACHKAY_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4023..6704)
                     /locus_tag="ACHKAY_004712"
     CDS             complement(4023..6704)
                     /locus_tag="ACHKAY_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO5703241.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6945..>7328)
                     /locus_tag="ACHKAY_004713"
     CDS             complement(6945..>7328)
                     /locus_tag="ACHKAY_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO5703242.1"
                     /translation="SNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNR
                     EGLLAANGAVRLDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAI
                     PGWQFLTQPSPEEQHFTWGNGCKKQ"
BASE COUNT         1972 a         1865 c         1446 g         2045 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgtct ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcattgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcgtttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agttccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttccct gcccagtacc
     1981 tggcgatgaa aaccatccac caccaagtaa tggttctttt cctcagacac taccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 acaggctcct gtgcaaaagg actgacctca tgaagcaccc tgcgcaggtg attaagggcc
     2221 tgtatggcct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaagt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc cttcatacgc tgcaggtaac
     2341 gttcatagtg ccggggtttg ttagggctaa aggagagggt ccggcaccag aaatcattct
     2401 ttatgagagt cttacagata cgtcgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cggaatgtca tccgggaagc cccgcgtctg ataccaacgc aggtagacag
     2521 caattttatt acggtaatgt tctgctgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga tgaggcggct tcgtgatacg cttacacagg gcaaaatagg
     2641 caccactctc attggcataa agcgccccgc tggcagcccc tgacacccgt tcacacatcc
     2701 tggcccaggt ttccggctcc agaacatggt aaagccacag tcccttacgc tgttcaggac
     2761 caaaaggttc acagacacgc atgttgcgta acggcacgcc ggcacggtac atcaggtcat
     2821 acagaggatt gtaaatggca caggttctgg cgttatatat ccagatatcg cgggccttcc
     2881 agtcatacaa cggatacatg gtgtaataaa acccttcagg cgatgccgtg gtccagggct
     2941 tatcatcggc atatctcagt ttgcgctgag ataccagtcc cataaagcga ttgagcgatt
     3001 catcagcacg gacaccagtc agtattgcca ctccacaccg gttaccggca aaccaggaag
     3061 aaaatgccgg aacaaattct tcaaacgtca tggcataccg gtaaaaggga aaatacgtca
     3121 tgtctgtaat ggcctcttcc ggtggctggc gaacccaggt cactcctggc tcccagcata
     3181 tccactccgg ctgaaactgc gagacgccgt ttaccgtggt cagggaagtg ccacccagta
     3241 aaaggtttcc gtcacatcat ggtacatttc ccgcatcttc tgaatatgtt caatagtgca
     3301 ccgatactgg gcctcccagt caatgaacag aacagagaaa tgacgttttc tcctgcgggc
     3361 cacctctgcc acaagatgga aaagcacagt ggaatccttt ccacctgaaa aagacaggca
     3421 taccgacgga aatgtttcaa aaatccactc aatgcgggcg attgcggcac gcaaaacatt
     3481 ttctccggta aggatttttt cggttaaaag cgtatcactc attttcctgg ctttatgtcg
     3541 aataattctg tttatgcatt cagacaaact tcattaataa ctcaccgctt tggggataaa
     3601 taataacaat aaaactcttc agatattaat ctggttgttg gatttgttat cacatccttt
     3661 atccacctgc ttcttactgg taacacatct ggaaaatcca tgcattttca acatatacca
     3721 tcctttcaac ttgtaaataa ggaaatatga tgcagatggt gaacaattca aatatcattt
     3781 tgcaggaagt attcactgta atagaattac cccctgataa agtttattca ctaaggaatt
     3841 attcaacaga accattccct gttagctttg agcaatagca cttattttta tatgtatcag
     3901 gaatacgtac gttgatgtaa ccaaaacaaa gaagcctgtg tccatggggt tcgtacaatt
     3961 gtgtgtggca catgaccaaa tcaggtttga gacctcccgc tgcaatattt tttattcaga
     4021 ctttaacctg tcaggcctga cagaaacgct cccaacttcc cttctatatc actgtctata
     4081 gtacgtaatc ttagcaatgg caggccgcat ttcttcagta tactgttttt aagttcatca
     4141 cgttcagcct gaacactggt cagatgataa cctccatcca cttcaataac ccctaatgga
     4201 gtcttcccga ccttatagta aacaacaaag tcacaactgg cccggttacg catgaataac
     4261 tgttcacggt gtgtaaaatc cccccgctcc agcaacacca actgattcag cgctatttgt
     4321 gaatgaaaca tcatactccg ataactatcc tgcgacagga tatcccgcaa caggcaggcc
     4381 acaatctgtt ctgacttaaa gtgtgaatca tttgaattca atctactatt cagtcgttcc
     4441 agtgatttgt catactcact gtacagcaaa tcaaatgctg atatgaccgg gctctcgaaa
     4501 atctctccgt catcagcgta gtatttgatg taacgaatca gcgccgcaat atgtccagcg
     4561 tggcgttcaa atacatcatt accagtcacc agcgtaaatt tatttcgggc ccttgaaaca
     4621 gcgacattca ccagttcagg attatcaaca aacgcgatgt tccgactgtg ctggctgctg
     4681 cgcttcttat ccagcacagt ggaaaacaca atttcatcgc actcccgtcc ctgaaattta
     4741 tgcaccgtcg atttcacaaa atcagcaggc agcactgttt cagccagatt aacctgtgca
     4801 ttatatgggg cgatatatcc cctgctactt tcctcatccc agtaatgtcc ttccagagat
     4861 tcaagctccc tgagatttga aaaattacgg gtatggtttc cttttgccgt gatgatcaga
     4921 gataatgagg cttcacctga atcaagcgtt agcggtatca gagcattatc ataaaactgc
     4981 ttattacaaa attgaatgat tttaggatga cagcgataat gctctttaag aagagttact
     5041 ggcaccatat tcctgaacaa catacaaacc gaatcaagta gactatattt ctcacaatta
     5101 tagtattctg ccggtggaga tgggctgttc gggagtaata caggaacatg tggaagttgc
     5161 tttcgatccc ctacaacgat tacattacgg gcacatccca gtcccagaat gcccggaaca
     5221 atatcctgct gtgaggcttc atcaataatc acataatcaa gaagagcccc cttaccgata
     5281 gagttaataa tagaatgcgt gctgctacca ataaccggaa aacgctttat aaaacggtta
     5341 aattcatccc tgtatgtctc tgccgagaat gaatttcttg ttgaaatatt tcttcgaaga
     5401 tgttgtttta aataaagcac agaccatgat gtcaggcgct ccagtaaaat tttaaaatca
     5461 gattccttta atacttcctc atattcattc agagaaactt gtttttcccg gatgaatttt
     5521 tcataataag aaagctgaag agaagtaaat agtgccagcc ttctctccgg aatcaccaat
     5581 ggtttgactt tcagtatcct gaagtttaac aacaaatcaa ttctgtttct gaatccaatc
     5641 cgcctgtcag aaaggtaatg aatataagcc atcaggtcag ttgtttttcg tgaggaaagt
     5701 cggtatttgt gagaaaaaag ctcctccggt cgaagattct gactctgcca ttgctgcagg
     5761 tatttatact caatattcag ctcattaatt tctgctttta acgaagcgac ctggttaatg
     5821 gcattcaggt gttttttcac ttgttgaagt acatcatcaa tcgcatttgc agacggtgag
     5881 tcaggtaaga cttcctcaga acgcgaaatc gacgttgaaa aaaactgttg cctgttctcc
     5941 gtactcccca gtcgtgccac cacatatccc agttgctgtt tgtccatctt ttcataaaca
     6001 ttactgacag ctgagttatt attggacaaa attgccactg ttttattctg aattaatata
     6061 ttagcaacaa tattgagtat agtctgtgtt ttacctgttc caggtggtcc ttcaataact
     6121 gaaacctgtg aggaaaatgc ccgttcgaca gccagcaact gactttcatt aagaccaaac
     6181 ggaaaaatca aaccccatgg cgaatcacgt tttttactct gtcctgaaca gtatgcatgc
     6241 aaggcggtat ccggatgagg aactattttc tttatctgcc taaggatatt ctcatcgata
     6301 tttctgttat taccgcgggc ataaaaaata cgttcattag ccaccctaca caagtaatta
     6361 aacacggcat gttctgtaaa tgctgaacac tgttgaaagt caagaccggt cgttttcatg
     6421 atgtagggtc gttcaccctc gcgataataa acagcagcat atttatcacc atatataata
     6481 accagttcag cccgggatgt tacagcgttc cctttccttt cgaggaaaac atttcgcaaa
     6541 tttacagttg gcgttatctg acatacagaa agcggacgag tatattttct cccgctgcga
     6601 aaatgacagg tcagcataac ctctctgtcg tcatttccag cccacagcgt ccagtcggta
     6661 attttatgag tcttattttc accgtcaaca caaatcgtga ccatattctt actcagaaat
     6721 tatcatttaa cttacggaaa attgttaagg cactcccgat attagctccg gaggattttc
     6781 cacaactccg ggactgttaa catatttctg cctgccatat atacattgaa tttagtcata
     6841 acagtcaata ccagaattac aggaatgcga tgccgatacg ctggttattg aatactggta
     6901 gcccctgagc actaattcat cacgatactc tgtatttact gttttcactg ctttttacaa
     6961 ccattccccc aggtaaaatg ctgctcctcc ggtgatggct gtgtcagaaa ctgccatccc
     7021 ggaatagcca cgctgaccgc cagcaatgcc gcacagacac cagccacaaa cggtttcaga
     7081 aacgtgggcg gggatttctg aagggagggt gactgctccg cctgcacttt ccccttgcga
     7141 ttatcaaggc tcagtgcatc cagacgcacc gcgccatttg ccgcaagcag cccttcccgg
     7201 ttatccacgc ttcccggcag actcagcgaa aaatcccctg ttcccgtctg aatcagactg
     7261 cctttacgat ttgacaggct ttgtgccgat atctgcagtg tgtcggcatt tatcgtaccg
     7321 ccattact
//
LOCUS       DAZFKM010000194         7325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFKM010000194 DAZFKM010000000
VERSION     DAZFKM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..416
                     /locus_tag="ACHKAY_004714"
     CDS             <1..416
                     /locus_tag="ACHKAY_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="colicin D domain-containing protein"
                     /protein_id="HFO5703243.1"
                     /translation="TPVSDAGGLRDFIYWRPDATGTGVEAVYVMLNDPLDSGRFSRKQ
                     LDKKYKHAGDFGISDTKKNRETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPN
                     TNNVVILKSNGEFLSGWKINPDADNGRIYLETGEL"
     gene            413..676
                     /locus_tag="ACHKAY_004715"
     CDS             413..676
                     /locus_tag="ACHKAY_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFO5703244.1"
                     /translation="MNKMAIIDLAKSFLASKITAMEFSERICVERRKLYGVKNLSQDV
                     LNCGEELFIAAERFEPDSDRADYEFDDNGLRIEVQSILEKFKL"
     gene            746..892
                     /locus_tag="ACHKAY_004716"
     CDS             746..892
                     /locus_tag="ACHKAY_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO5703245.1"
                     /translation="MKKKIGVSIILVLVVLCLTACQNNYVRDVQGGTIAPSSSSKLTG
                     VAIQ"
     gene            complement(1121..1234)
                     /locus_tag="ACHKAY_004717"
     CDS             complement(1121..1234)
                     /locus_tag="ACHKAY_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5703246.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDFTPLAAAIGN"
     gene            complement(1904..2095)
                     /locus_tag="ACHKAY_004718"
     CDS             complement(1904..2095)
                     /locus_tag="ACHKAY_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5703247.1"
                     /translation="MTKQEKTALNMARFIRSQTLILLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            2162..2485
                     /locus_tag="ACHKAY_004719"
     CDS             2162..2485
                     /locus_tag="ACHKAY_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFO5703248.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGHWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            2694..3128
                     /locus_tag="ACHKAY_004720"
                     /pseudo
     CDS             2694..3128
                     /locus_tag="ACHKAY_004720"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3181..3723
                     /locus_tag="ACHKAY_004721"
     CDS             3181..3723
                     /locus_tag="ACHKAY_004721"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFO5703249.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRD
                     AISAHTAALKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTALCVT
                     GGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSG
                     VKGENNWTVEKKNAVRLVRE"
     gene            3730..3963
                     /locus_tag="ACHKAY_004722"
     CDS             3730..3963
                     /locus_tag="ACHKAY_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFO5703250.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
     gene            complement(4448..4867)
                     /locus_tag="ACHKAY_004723"
     CDS             complement(4448..4867)
                     /locus_tag="ACHKAY_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO5703251.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
     gene            complement(4951..5388)
                     /locus_tag="ACHKAY_004724"
     CDS             complement(4951..5388)
                     /locus_tag="ACHKAY_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFO5703252.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGR
                     REFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
     gene            5646..>7325
                     /locus_tag="ACHKAY_004725"
     CDS             5646..>7325
                     /locus_tag="ACHKAY_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFO5703253.1"
                     /translation="MSDNEGSVPTEGIDYGDTMVVWPSTGRIPGGDVKPGGSSGLAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRSGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPVSQLPA
                     AGNNVPVKYRLTDLMQNGTQYIAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDRNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKVELFSKAGV
                     KQPPVYTQEMVERANSVMNEQGTLVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVGPLLMRISLGALWFHSEDAGAGSDIVPGRDMEAMFSLNAQMLAGQGM
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNDSIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPAPQPSAPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTI"
BASE COUNT         1816 a         1611 c         2068 g         1830 t
ORIGIN      
        1 ttactccagt ctctgatgcc ggtggacttc gagattttat ttactggcgt cctgatgcta
       61 ccgggactgg tgttgaagct gtctatgtga tgcttaatga tcctctggat tcagggcggt
      121 tttcacgcaa acagcttgat aaaaaatata aacatgctgg tgattttggt attagtgata
      181 ctaaaaagaa tcgtgaaact cttactaaat tcagggatgc tattgaggaa catttatccg
      241 ataaggatac agtagagaaa ggaacatacc gacgagaaaa aggatcaaaa gtttatttta
      301 atcctaatac aaataatgtg gtgatactaa aatcaaatgg tgagttctta tctgggtgga
      361 aaataaatcc agatgcggat aatgggcgaa tttatttaga aacaggtgaa ctatgaacaa
      421 gatggcgata atcgatttgg cgaagtcatt tttagcttca aaaataacag caatggagtt
      481 ttcagagcgg atttgtgttg agcggagaaa attgtatggt gttaagaatt tgtctcagga
      541 tgtattgaat tgtggggagg agttgtttat agctgctgag cgtttcgagc ctgattctga
      601 tagggctgat tatgaattcg atgataatgg acttaggatt gaagtccaat ctatcctgga
      661 aaaatttaaa ttataaatat gagtgatttt aatatctgga atttaattat aaatgtcaga
      721 tactcactca tgacaaggag atgttatgaa aaaaaagata ggggtatcga ttattctcgt
      781 tcttgttgtt ttgtgtctga ctgcttgtca aaataactat gtaagggatg ttcagggagg
      841 aacgattgct ccatcatcat cctcgaaact aaccggggta gcgattcagt gaaaaagatt
      901 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
      961 ccaccgctac caacggtggt ttgtttgccg gaacaagagc taccaactct ttttccgaag
     1021 gtaactggct ttagtggagc acagatacca aatgctgtcc tttcagtgta gccgcgataa
     1081 ggcttcaact tcaagagttc tgcgtatcta aatccacttt tcagttacca atggctgctg
     1141 ccagtggcgt aaagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1201 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1261 acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1321 gaaaggcgga caggtatccg gtaatcggca gggtcggaac aggagagcgc acgagggagc
     1381 ttccgggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc tctgacttga
     1441 gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta tggaaaaagc gccaccggcg
     1501 cggccttttc cccggctttg tcttgttgct cacatgttct ttccggcgtt atcccctgat
     1561 tctgtggata acagtattgc cgcttttgtg tgagcagaca ccgctcgccg cagccgaacg
     1621 accgagcaca gcgagtcagt gagcgaggaa gcggaagagc atcggcatct gcatgttctc
     1681 cttacgcatc tgtgcggcat ttcacaccgt acatggtgca cttttcatac aatcctcact
     1741 gatgccacac gattaagcga gtatacactc cgctatcgct acgtgactgg gtcatggctg
     1801 cgccccgaca cccgctaaaa cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1861 ccgctcacag gcaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1921 catccccgaa gcgtgcgagg cagctgcggt aaagctcgtc ggcgtggtcg tgaagggctt
     1981 cgcagatgtc tgcctggtcg tcggcgtcca gttcattcag tttttccagc aggatcagcg
     2041 tctggcttct gataaagcgg gccatgttaa gggctgtttt ttcctgttta gtcacagttc
     2101 tgttctccat gtgtggggag tctgtcggag gtttttaatg ataccggtga aacgcgaaag
     2161 gatgctcacg atacgggtca cggatgagga acatgcgcgg ctgctggcac gttgtgaggg
     2221 aaaacaactt gcgtcatgga tgcgtaaggt ctgcctgggt gcaccgccat cgaaaacgtc
     2281 aggtcttccg acactggcac cgccgctgtt gcgccagttc gcttcggtcg gcaataacct
     2341 gaatcagata gcccggaaga taaacagcgg acactggtcg gggcatgacc gggttcatgt
     2401 ggtggctgca ctgatggcca tcggacgtga actgagtgag ctgagggatg aggtccgtaa
     2461 acagggagaa cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     2521 cggttgatta cctgctgggc cgggagcgta accgggaagg ggcgagaatt ctgcggggtg
     2581 atcccgaaga ggtgcgggaa ctcatcgatg ccacgccgtt tgcgaaaaaa tacacctcgg
     2641 gggttctgtc ttttgcagag cagacactgc caccgggaga gcgtgaaagg gtgatggaga
     2701 gctttgagcg ggtactgatg cccggtcttg aaaaaaatca gtacagcatc ctgtgggtgg
     2761 agcaccagga caaggggcgt cttgagctga attttgtcat cccgaatatg gagctggtga
     2821 gcggaaaacg gctccagccc tattacgacc gtgcggaccg tccgcgaatc aatgcctggc
     2881 agacactggt gaatcatcat tacgggctgc acgacccgaa cgcaccggag aacaggcgga
     2941 cacttgtcac cccgaataat ctgccgaaag cgaaacagga ggccgcagaa gcgattacga
     3001 gggggctgga ggctctttat catgccggag cgctaaaaac gcgtcaggat gtcactgagg
     3061 cgcttacagc ggcaggtttt gaggtggtga ggacaaccag gagcagtatc agtatcgccg
     3121 acccggaggg cgggcgtaat ctcaggctga aaggggcgct gtatgagcaa tcttttacaa
     3181 atggggacag acttcgagaa gaaactgaaa gagcgtgccg tctctacaga gaacatgctg
     3241 aacagcgagt tcaggaagct cgaagcgtct gtagacaggg aactgaactt aaacgggcag
     3301 aaaatcagag acgctatcag cgcacacaca gcagcgctga aagaacaact ggagacgctg
     3361 aacagtgccg tgaacgcgca gttcagcgcc acagagaacg aactggcccg gcggcaggaa
     3421 gagctgctct ggaagctgat caaagggcgg atactgtacc cgtcgctgac ggcgctttgc
     3481 gtcacgggcg ggatttttct ggcgtcatgg gggctgattc agtggcagga aagccggatt
     3541 gcggcgaaca tcctggctat cagggaccag gaagagacgc tggcgaaact ccgggaaaaa
     3601 acatggggtg tgacgtatca cgagggcagg aacgggaaat ttctggtatt gccgtccggg
     3661 gtgaaggggg aaaacaactg gacggtggag aagaagaacg cagtaagact ggtcagggag
     3721 taagtgagta tgacagagct ggaaatgcac ttactgaacg ccttagagca attgcagcag
     3781 gactatacgc agcggctgaa agactgggag agcgccttca cggatttgca gcatatgttc
     3841 gagctgacac gacaggagaa cgggcaactg agggaacagt gcgacgactt gagtcagcag
     3901 gtgcagagat tagccgggca ggtggaccgc ttgagtcggt tatttatcac gaacagcaga
     3961 taagggatga acaggaaagg ctggcacgcc tggaacggga gcgtgcgcgg tcctggcatg
     4021 aagagcagga acgggaagta cgggtatggc gtggcccgtc aatggggatg tgacggtgag
     4081 ttgttccggg aggttgccag atggtaccgg aacgcgttaa aaatgcatcc gggcgcgatt
     4141 ctggcgtgtc gtttttgttg ctgtgttttg cctctttgcc agatcgcgct cattcctcag
     4201 cctcatcccg cgatgtctgc gtatgtcatc aactgaaaag atggaaaaaa aacagtgtca
     4261 tgaggtttac gcaggcaaaa ccagcgttat tcacatggct gaatagctat gcatacggtt
     4321 aaaatttatc aggtgcgatc gcggcagctt ttcgggtggt ttgttgccat ttttacctgt
     4381 cagcagccgt gatcgcgctg aacgcatttc agcggtgcgt acaattaagg gattatggta
     4441 aatccactta ctgtcttccc tcgtagccat cgagataaac cgcagaaatc gtctcagcca
     4501 gcagcctgga ttttttcggg gtaaggtctt tgctgcggta atcagtaccc gcaaattgag
     4561 ttgtgccccg gagtgagttt aaaaattcct ggctgtgaaa ctgctgaatg cgttgctgca
     4621 catcactccg tgattttcct gtcgctttat cctttgctcc catatcccag aactgccgga
     4681 aaaaaggcac catcattcgt gttgtgtcgg ccttctgggt ggaaaagtta ggatcaaaac
     4741 catcgtctga tgcataaacg taatgccggg tatgttgttc cacactgccc tttggcgtgc
     4801 aggctgtcag gaccgttgcc gctgtcagca gggcgataag atacagtgtg ccgccggaac
     4861 gtctcatgag tgtctcctta cctttaaatc tttgtactca gtgacttata tttaaccgct
     4921 gactatctta tcatcacttg cagccgatgc tcaccggctg aacagtctgg cccagaatcc
     4981 ccgttttttt tcctgctcca gtgcctgcct gagctgctct atctctttat gtagctgttc
     5041 ccgttcggct tcctggcgcc ggcgttccgt atcctgtgcc tgtttgtcct caagcatcag
     5101 tgtcaggcat tgtttcagct cattcagctc ccggagaatg cgttctgtct gctgatcatg
     5161 tggattttct gcctgtcccg cactgtgcct ttctgctgtc tcatcctgct ttaattcgcc
     5221 gtatgcccgg atcagttcac tggtttcaaa ctcccgtcgt ccgtcctggc cggtacggta
     5281 actgacaagg cctgcgcgca tatcacggta aaactgtgaa cgcgatctgc ctgtcatttt
     5341 cagtgcgtcc tggagtgtgt gccatgccat gaagtgacag tgtcccattg gtgtctcata
     5401 tcatggtttc agtaaaacat actggggccg gagaacggta atgtttgtgc tggtttttgt
     5461 ggcatcgggt gggaatagca cgtggtgtgg gtggctgttt ttttgatcgt tttcacaaaa
     5521 acgaaaatcc acagggttga cagggaaaac gcagcggcgt aattttaaac tgtatataaa
     5581 accagtggtt gtatatacag tatgcgtctt ttaactattt gtttttaatg tcaaagaggt
     5641 tttctatgag tgataatgaa ggtagtgtcc cgacagaagg tattgattac ggggacacaa
     5701 tggttgtgtg gccgtcaact ggacgaattc cgggcggtga tgtgaaaccc ggtggttcat
     5761 caggtctcgc tccatccatg cctccgggat ggggagatta cagtccacaa ggtatcgcac
     5821 ttgtacaaag tgttcttttt ccaggaatta ttcgccggat tatccttgat aaggaacttg
     5881 aagaggggga ctggtcggga tggtctgtca gtgtgcatag tccctgggga aacgagaaag
     5941 tttctgctgc acgaacagtt cttgagaatg gtttacgtag tggtttgcca gaaccatcac
     6001 gcccggctgc tgtttctttt gcccgtctgg agcctgcttc cggaaatgag caaaaaatta
     6061 ttcgtcttat ggttacacag caactggagc aggtaacgga tattcctgtc agccagttac
     6121 cagcagcggg taataatgta ccggtgaaat atcgtctgac ggaccttatg cagaacggta
     6181 cgcaatatat tgctattatc ggaggtattc cgatgacagt gccggtagtt gatgccgttc
     6241 cggttccgga ccggagccgt ccgggaacaa atattaaaga tgtttacagt gcccctgtat
     6301 caccaaatct accggacctg gtattaagtg tggggcagat gaatactccg gttctgtcta
     6361 atcccgaaat tcaggaagag ggcgttattg ctgagacagg taattatgtt gaggcaggtt
     6421 atacgatgtc cagtaataat catgatgtca ttgttcgttt tcctgacggc agcggtgttt
     6481 ctccgttata tatttcaact gtggagattc ttgaccgtaa tggtctgagt cagcgtcagg
     6541 aagcagaaaa taaagcaaag gatgatttca gagtcaagaa agaagaagcg gtagcccggg
     6601 ccgaagctga aaaagcaaaa gtcgagttat ttagtaaagc aggtgtgaaa cagcctcctg
     6661 tatatacaca ggaaatggtg gaaagggcta attcagtaat gaatgagcag ggaactcttg
     6721 ttctgaataa cactgccagt tctgtacaac tggcgatgac cggaactggt gtctggacgg
     6781 ctgctggtga tattgcaggc aacataagca agtttttcag taatgctctg gagaaagtaa
     6841 ccatacccga agtgggtccc ctgcttatgc ggatttctct tggcgctctg tggttccatt
     6901 cagaagacgc cggagcagga agtgatatcg tgccggggcg agatatggag gctatgttct
     6961 cgctgaatgc tcagatgttg gcaggacagg gcatggtcat tgaacctggt gctacgagtg
     7021 ttaatctgcc tgttcgtgga caattgataa acagtaacgg acaacttgct ctggatttac
     7081 tgaagacagg gaatgatagt atccctgctg cggttcctgt tcttaatgct gttcgtgata
     7141 cagctacagg actggataaa attacgctgc cagcagtagt cggtgcacct tccaggacga
     7201 ttctggtaaa tccggcccca caaccttcgg caccaacaga tacaggaaat catcaacccg
     7261 ttccggttac cccagtgcat acaggtacgg aggtaaaacc ggttgaaatg ccggtaacga
     7321 cgatt
//
LOCUS       DAZFKM010000195         7298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFKM010000195 DAZFKM010000000
VERSION     DAZFKM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(413..1479)
                     /locus_tag="ACHKAY_004726"
                     /pseudo
     CDS             complement(413..1479)
                     /locus_tag="ACHKAY_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1482..1844)
                     /locus_tag="ACHKAY_004727"
     CDS             complement(1482..1844)
                     /locus_tag="ACHKAY_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5703254.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2381..4294
                     /gene="mtlA"
                     /locus_tag="ACHKAY_004728"
     CDS             2381..4294
                     /gene="mtlA"
                     /locus_tag="ACHKAY_004728"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO5703255.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4524..5672
                     /gene="mtlD"
                     /locus_tag="ACHKAY_004729"
     CDS             4524..5672
                     /gene="mtlD"
                     /locus_tag="ACHKAY_004729"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO5703256.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5672..6259
                     /gene="mtlR"
                     /locus_tag="ACHKAY_004730"
     CDS             5672..6259
                     /gene="mtlR"
                     /locus_tag="ACHKAY_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO5703257.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6271..6480)
                     /gene="yibT"
                     /locus_tag="ACHKAY_004731"
     CDS             complement(6271..6480)
                     /gene="yibT"
                     /locus_tag="ACHKAY_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO5703258.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6765..7127
                     /locus_tag="ACHKAY_004732"
     CDS             6765..7127
                     /locus_tag="ACHKAY_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO5703259.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1867 a         1817 c         1839 g         1775 t
ORIGIN      
        1 tttttttaac ttagttttat aatgaatacg ataattaaaa gtgaagaaga aaatagttgt
       61 atacatatat tccatgaccc tacattgtga tctatagatt aaatattttt taaatgtgac
      121 tgtcatcctt cagataataa agccatcatc gcaatagatg gaaaagcact cctgctcttt
      181 tttgattaga gccgtcgcag gaggaacaac tcatatcatc attgcgatct cgacgatgta
      241 tagtctcatc ctgcggcaga acaagacgga taaaaaatcc aatgagagca cactcatcca
      301 gaacttatta acataatgaa tattaaagaa aaatcatcgc agctaatgaa gtaatatgca
      361 aaaataatta gtccttacaa ggagcaaagt tccataatct cccccctccc cctcaatgat
      421 ccaaataaag ataatgcatc cagctggtca ttcttagcag cactttccgc atcaccgaaa
      481 catggctgaa atggtcggag taaaccgcca cctgggcgta gatgccgtcg ggtaaggcat
      541 cgatatcatc gttagggtcc agttcaatgg ttcccagcac accgtccgtg ccgggcacga
      601 ccgttaatga ttgcaatacc ccctgcgcct gataagaacc gcctggcacg acaggtaaaa
      661 tactggtcag tttgccgtgg aacacctgcc caggtagcgc gttaaacacc acttctgcat
      721 catcaccagg tttcagacgt aacagcgagt tttgccgaaa ttgggcgaca atttgccgtt
      781 tttgctcggg gatgaaaacc atcaccggac gcaacggcaa ggcagctgcg tatgtacctg
      841 ggcggatcag tacctgagta atgtagccat tgctcggcgc gcgaatgaca gtctgctcaa
      901 gattatattt tgcttcagta agttgcgctc ttaagctcac aatctgcgat tgctcgccgt
      961 taaccatact gtcgagctgg ctctggatct gcgcctgctc cgccaccgag cctttcacca
     1021 gcgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat gtcacgttcc gagaacggat
     1081 tcaccgccgc ctggctgcct ttcagataac gttgataatt tttaaacaga cggtcgcgct
     1141 ccgctgaaac ctgggtggtg ttggcctgcg cttctgtgag ctgcgcgcgc agagtcttta
     1201 tattatgcgt cgccgtcatc aggtcagcct gaagcctgtc aactcgcgcc tggtaacgaa
     1261 ccgggtcgag cttaaaaagc acctcgccct tttgaataag ctgattattc ttgtcagtga
     1321 cttcagtaac aattcccgtc acctgtggcg tgatggggat cgctatcact gccttttgcg
     1381 cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat caaaccactc accagaaaca
     1441 cgccagcgcc acgtaagtta aaacaatcaa tagatccata attagatctc cgggaacgtg
     1501 gaatagtcag tctttttctc ggcggcgagt tgatgctcca gccgggaaat tcggtcagaa
     1561 agtgcggcga tttccgggtc agtcgctttc tcctgcgacg caacatgtga ctgcataccc
     1621 cagccacgct ccggttgata gagcgtcgcc cagatccaca gaaacggcca gataacatgc
     1681 agagtaaaca ggctcaccca gcccgccgta tgaatagcgt cggcatgggg atggttgcgc
     1741 tttttggcaa tcagataagg gatgtcatgt atcgcgatga ttccataaaa aatcaccaga
     1801 aagacgaaga tcagcactcc caacgcgaaa tagtttagaa acatatctgc ctcggattca
     1861 cgtttatcag tgttgttttt gggctggcag ccagaaggga gtcaggctga tattttgaca
     1921 ataatccggg ttcgcgattc tcgccataac accaaagaat aatttttaga ggtgatgagt
     1981 tgcttagtta cataacgatt gtatgacgaa ggcataacat gctgtagatc acatcaggtg
     2041 aacgccgtaa gaaaatatct tgtgattcag atcacaaaga ttcaacaaac catcaaaaca
     2101 aaaatgtgac actactcaca tttaaatgcc atttttagcg aaaatcgccg ccttgttgct
     2161 tttttacaca agcgttttgt gatgaacgtc acgtcaatta cctctctacc ccctatattt
     2221 atgtgattga tatcacacaa aaggccgtcg actggacagt taaccgattc agtgccagat
     2281 ttcgcagtat ctacaaggtc cggctacctc tgccgccaca ttaacaaaaa acctcgggct
     2341 tccagcctgc gcgacagcaa acataagaag gggtgttttt atgtcatccg atattaagat
     2401 caaagtgcaa agctttggtc gtttcctcag caacatggtg atgccaaata tcggcgcgtt
     2461 tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca gggtggttac cgaacgagac
     2521 gctggcgaag ctggtcgggc cgatgatcac ttatctcctg ccgctgctga tcggttatac
     2581 cggtggtaag ctggtaggcg gcgaacgtgg cggcgtagtc ggtgccatca ccaccatggg
     2641 cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct atgattgcag gtccgctggg
     2701 cggctggtgc attaagcact tcgaccgctg ggtagacggt aagatcaaat ccggttttga
     2761 gatgctggtg aataacttct ccgcaggcat catcgggatg atcctcgcta ttctggcatt
     2821 cctcggcatt ggcccgattg ttgaagccct gtccaaaatg ctggctgcgg gcgttaactt
     2881 catggttgtc catgacatgc tgccgctggc gtctatcttt gttgaaccgg cgaaaatcct
     2941 gttcctcaac aatgccatta accacggtat cttctcgccg ctgggtattc agcagtccca
     3001 tgaactgggt aaatcaatct tcttcctgat tgaagctaac ccaggtccag gtatgggcgt
     3061 gctgctggcg tacatgttct ttggtcgtgg tagcgctaaa cagtctgcgg gcggtgcggc
     3121 aatcatccac ttcctgggtg gtatccacga aatctacttc ccgtatgtgc tgatgaatcc
     3181 gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc gtgttcacgc tgactatcct
     3241 gggcggtggt ctggtttctc cggcatctcc gggttctatc cttgctgtac tggcgatgac
     3301 accaaaaggt gcttatttcg ctaacatcgc gggtgtgtgt gcggcgatgg ctgtctcctt
     3361 cgttgtctct actattttgc tgaaaaccag caaagtgaaa gaagaagatg atattgaagc
     3421 agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa ggcacatctc cgctgtctgc
     3481 gggcgatgtg actaacgacc tgagccacgt acgtaaaatc atcgttgcct gtgacgccgg
     3541 tatgggttca agtgcgatgg gcgcaggcgt gctgcgtaag aaaattcagg atgcaggtct
     3601 gtcacagatt tcggtcacta acagcgcgat caacaacctg ccgccagatg tggacctcgt
     3661 catcactcac cgtgacctga ccgaacgcgc tatgcgccag gttccgcagg cgcagcatat
     3721 ttcgctgacc aacttcctcg acagcggcct gtacaccagc ctaaccgaac gtctcgttgc
     3781 tgcccagcgc catactgaaa acgaagtgaa agtgaaagac agtctgaaag acagctttga
     3841 cgattccagt gctaacctgt tcaaactggg cgcggagaac atcttcctcg gtcgcaaagc
     3901 agcaaccaaa gaagaagcga ttcgttttgc tggcgagcag ctggtgaaag gcggttacgt
     3961 tgagccggaa tacgttcagg cgatgctgga tcgtgaaaaa ctgaccccga cttatctggg
     4021 tgagtctatc gcggtgccac acggtacggt tgaagcgaaa gatcgcgtac tgaaaacggg
     4081 cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt gaagaagaag atgacattgc
     4141 ccgtctggtg attggtattg cagcccgtaa caacgagcac attcaggtta tcaccagcct
     4201 gaccaatgca ctggatgatg agtccgtcat cgagcgtctg gcacacacca ccagcgtgga
     4261 tgaagtgctg gaactgctgg caggtcgtaa gtaatccaat cccaccctct ccacatggag
     4321 aaggtggggt taattgcctg atgcgctacg cttatcaggc ctacaggatg catcacaatt
     4381 tgttgaattt gcacgttctt gtaggccgga taaggcgctt acgccgcatc cggcgctgcc
     4441 cctctcctca cggagagggt ttgggtgagg gaaaagcctc accccagccc tctcgggtaa
     4501 aaacattgat gaaggttaat actatgaaag cattacattt tggcgcaggt aatatcggtc
     4561 gtggctttat cggtaaactg ctggcagacg cgggtatcca actgacgttt gccgatgtca
     4621 atcaggtggt acttgatgcc ctgaatgccc gtcatagcta tcaggttcat gtggttggtg
     4681 aaaccgagca ggtagatacc gtttccggcg tcaatgctgt cagcagcatt ggtgatgatg
     4741 tcgttgatct gattgctcag gtcgatttag tcactaccgc cgttggcccg gttgtgctgg
     4801 aacgtattgc tccggctatc gccaaagggc tggtgaaacg caaagaacaa ggtaatgaat
     4861 ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg taccacgcag ctgaaaggcc
     4921 atgtgatgaa cgccctgccg gaagacgcca aagcgtgggt agaagaacac gttggctttg
     4981 tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc ggcaactaac gatccgctgg
     5041 aagtgacggt agaaactttc agcgaatgga ttgtcgataa aacgcagttc aaaggcgcac
     5101 tgccgaacat cccaggcatg gagttaaccg acaacctgat ggcatttgtc gaacgtaaac
     5161 tcttcaccct gaacacgggt catgctataa ccgcgtacct cggaaaactg gccggtcatc
     5221 agaccattcg tgacgctatt ctcgacgaga aaatccgcgc ggtggtaaaa ggtgcgatgg
     5281 aagaaagtgg tgcggtactg atcaagcgct acggctttga cgcagacaaa catgcggcgt
     5341 acatccagaa aatcctcggt cgttttgaga acccgtatct gaaagatgat gtagagcgcg
     5401 taggccgtca gccgctgcgt aaactgagtg ctggcgaccg tctgatcaag ccactgctcg
     5461 gtacgctgga atacagcctt ccgcacaaga atctgattca ggggattgct ggtgcaatgc
     5521 acttccgcag tgaagatgat ccgcaggctc aggaactggc agcactgatc gctgacaaag
     5581 gtccgcaggc ggcgctggca cagatttccg gtcttgatgc caacagcgag gttgtatccg
     5641 aggcggtaac cgcttataaa gcaatgcaat aatggtggac caggcgcagg acaccctgcg
     5701 cccgaataac agattgtcag atatgcaggc aacaatggaa caaacccagg cctttgaaaa
     5761 ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga agctttctga tcaccgccgt
     5821 cgagcttttg accgaggcgg taaatcttct ggtgcttcag gtattccgca aagacgatta
     5881 cgcggtgaag tatgctgtag aaccgttact cgacggcgac ggtccgctgg gcgatctttc
     5941 tgtgcgttta aaactcattt acgggttggg cgtcattaac cgccaggaat acgaagatgc
     6001 ggagctgctg atggcattgc gtgaagaact aaatcacgac ggcaacgagt acgcctttac
     6061 cgacgacgag atccttggac cctttggtga actgcactgc gtggcggcgt taccaccgcc
     6121 gccacagttt gaaccagcag actccagttt gtatgcaatg caaattcagc gctatcaaca
     6181 ggctgtgcga tcaacaatgg tcctttcact gactgagctg atttccagaa tcagcttaaa
     6241 aaaagccttt caaaagtaag caacgtctgc ttactgcccc tctacctgct tcggccgata
     6301 aagccgacga taatactcca gacgttgtag atataacggc acgctttcat cgggtattcc
     6361 ggacggaatc gcgttacggg gaggaagttt tttcagatac tcccggaacg cctggcttga
     6421 tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta atttgcccat
     6481 gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt agtttaggtt
     6541 cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc aataatcatc cgctgctgtt
     6601 tattatcttt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
     6661 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
     6721 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
     6781 aaaacgaaat caaacgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
     6841 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
     6901 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagcaaa gaagcacaga
     6961 agctgatgaa gatgccgttt cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
     7021 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctgggccgtg
     7081 aaatgggcct gcaggagatg actggattct ctaagactgc gttttaagcg cctcagacga
     7141 ctggttcgat catatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
     7201 ctgtctactc gcctaattac caaacaattc actcccttta cgtcgattta ccgctggttg
     7261 atattctgaa aatgcatttt taccgaaata gccaccta
//
LOCUS       DAZFKM010000196         7293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFKM010000196 DAZFKM010000000
VERSION     DAZFKM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHKAY_004733"
     CDS             complement(<1..114)
                     /locus_tag="ACHKAY_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703260.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHKAY_004734"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHKAY_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO5703261.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHKAY_004735"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHKAY_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO5703262.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHKAY_004736"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHKAY_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO5703263.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHKAY_004737"
     CDS             complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHKAY_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO5703264.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1708 a         1962 c         1956 g         1667 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcaccgtc ataactctgt cagaaagcat acctaacaga
     6241 caatcttgca cttccagcgg tgtacgcgtt atctcttcga aacggacgat tttgccgtcg
     6301 cgcattccct ggtaaagcgg ggcagggacc agcgcttccg ttgatgggcc atgattgata
     6361 agcagagcgt agttccagcc gtacttgatt tgatcttcag tggtagatgc gccgccctgg
     6421 atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag gagagatttt
     6481 gcggtgccag gttcccccac caacatcaaa ccgcgtccgg tggcaagagt gactaacata
     6541 cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc gctatcgcca
     6601 aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtta
     6661 tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg cggacgctgc
     6721 agatgattat tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat
     6781 attttacgcg caataaatgt ttggcgcatt agttgtttga gcggaaaata tctggaaagc
     6841 agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta ctattttcag
     6901 gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa ccacggctgg
     6961 accccgttgc aattaagata atgagattta taatcctgac tgttgggacg ttgcgccgga
     7021 tcgaacctat ttcaagtcct ggctaccgga cggggggtgc tgcaggtggt gccctgatcc
     7081 ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt gtaagggtaa
     7141 ggtgctggtg ttgcgctcct taataaggag agcggatgga ctcgctgaca caaaagttaa
     7201 ccgtgctcat tgctgtactg gagctattag tggctctgtt acggttgatt gatttgttga
     7261 agtaacgggc aaattgaaac gcatctaagg ggg
//
LOCUS       DAZFKM010000197         7181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFKM010000197 DAZFKM010000000
VERSION     DAZFKM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(327..1976)
                     /gene="pgi"
                     /locus_tag="ACHKAY_004738"
     CDS             complement(327..1976)
                     /gene="pgi"
                     /locus_tag="ACHKAY_004738"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO5703265.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      2213..2409
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2501..3850
                     /gene="lysC"
                     /locus_tag="ACHKAY_004739"
     CDS             2501..3850
                     /gene="lysC"
                     /locus_tag="ACHKAY_004739"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO5703266.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3995..4921
                     /locus_tag="ACHKAY_004740"
     CDS             3995..4921
                     /locus_tag="ACHKAY_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO5703267.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4957..5748)
                     /locus_tag="ACHKAY_004741"
     CDS             complement(4957..5748)
                     /locus_tag="ACHKAY_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO5703268.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5754..6881)
                     /locus_tag="ACHKAY_004742"
     CDS             complement(5754..6881)
                     /locus_tag="ACHKAY_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO5703269.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1793 a         1851 c         1708 g         1829 t
ORIGIN      
        1 aaatcataaa cttaaaataa aaacaaaagc aattaatctg cgtattttcc gaaataaaca
       61 acgattaaaa atctatttta ggattaacct catggtatga tttccgttaa atcacagaca
      121 aggggattta tctgataaaa aaatgccgac ataacgtcgg cattgttatt aaggttattt
      181 aatcaatcgt gctatgtatt aattgccgaa tacgatgtac atatcggcat cgacctgtag
      241 gcctgataag acgcgaccgc gtcgcatcag gcatcggttg ccggatgcgg cgtgaacgcc
      301 ttatccggcc tacatatcga cgatgattaa ccgcgccacg ctttatagcg gttaatcaga
      361 ccattggtcg aactatcgtg gctgctgatt tctttgccat ctttcagctc tggcagaata
      421 cggttcgcca gctgtttacc cagctccacg ccccactggt cgaaggtgaa gatgttcaga
      481 atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc caggctgaac
      541 ggagtgattt cacgcagcag gatggagttg gtcgggcggt taccttcgaa cactttgaac
      601 ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc ctgctcaacc
      661 acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt agacagcagt
      721 ttctggtgat gatcagagag cgggttatgg gtgatagccg gagcgatgaa atcgcacggt
      781 accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt agtgcctggt
      841 tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg gtcaacatac
      901 ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg catatactgg
      961 tcatacggca gaatcgcctc agtttccgca ccaaagaaat tgttgtacca gatgccaacc
     1021 agcgccagca gtacaggcag gtttttctcg gcaggcgtgg tggagaaatg cttgtccatc
     1081 gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag aacaatcgac
     1141 aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa ctcgaacatg
     1201 ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag cgccgcaaag
     1261 tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg cgcgctatgg
     1321 gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa cagcgtggtt
     1381 tccgggttta cttttttcag cacttcagcg atgtgagtcc catcgacgtt agaaacaaag
     1441 tgcatgttca ggtgattttt gtacggacgc agtgcttcgg tcaccatgta cgggccgagg
     1501 tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt ataacccttc
     1561 cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac cgcgttgact
     1621 tccggcatta cgtctttgcc atcaaccaaa atcggggtat tgctacggtt acgcagcgct
     1681 acgtgcagca cagcgcggtt ttcagtgcgg ttgatcttct cgccagagaa catcgactta
     1741 atcgcgcccg ccagatcgca ctctttcgcc agatcttgta atttcgccag cgtctcttca
     1801 gtgatgcggt ttttggagta atccaccagc atctgatcgc cgaaggttgc ggagaactta
     1861 gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt catttcatcg
     1921 aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt tttcattagc
     1981 aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag tcacagaaaa
     2041 atgtgatggt tttagtgccg ttagcgtaat gttgagtgta aacccttagc gcagtgaagc
     2101 atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg accccgtcgt
     2161 tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag cgcaggccag
     2221 aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata acacctgagg
     2281 gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta caggggctga
     2341 atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg gtggagcact
     2401 tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg ctcttccctt
     2461 gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa ttgttgtctc
     2521 caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg ctgatattgt
     2581 gctttctgat gccaacgtgc gtttagttgt cctctcggct tctgctggta tcactaatct
     2641 gctggtcgct ttagctgaag gactggaacc tggcgagcga ttcgaaaaac tcgacgctat
     2701 ccgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta tccgtgaaga
     2761 gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc tggcaacgtc
     2821 tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc tgctgtttgt
     2881 tgagatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta aagtgatgcg
     2941 taccaacgac cgatttggtc gtgcagagcc agatatagcc gcgctggcgg aactggccgc
     3001 gctgcagctg ctcccacgtc tcaatgaagg cttagtgatc acccagggat ttatcggtag
     3061 cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata cggcagcctt
     3121 gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc cgggcatcta
     3181 caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg cgtttgccga
     3241 agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt tgctacccgc
     3301 agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg caggtggtac
     3361 gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc ttcgtcgcaa
     3421 tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct tcctcgcgga
     3481 agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca cgtcagaagt
     3541 gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata cgttgctgac
     3601 gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaagtagaag aaggtctggc
     3661 gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca aagaggtatt
     3721 cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca gccataacct
     3781 gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg cagaagctgc attttaattt
     3841 gtttgagtaa tatttcccta ctggcgtata acgctcaccg attgttatac gccatcctca
     3901 ctgaaaacat agattaaata atatctgaaa agcaaaatca ctcctgtaga acagtattat
     3961 aatcaccgaa aaatatcatt taacaggagt gttaatggct actttaatgg aaaaagatgc
     4021 acttttaaac ggagccagtc agtgcatcgc gtttttatca aatatcattg ataattgctc
     4081 agtttcgtcg catcaggatt caggcgatgc tctcaagcga ttagtcagct atcgcgatta
     4141 tctttattct acccccgctg aacttgttga ttttacacaa ggtaagattc tccttcagca
     4201 ggtaagaact caatatcagc atgaattcaa caatactacg cattcagaaa ataaagcatc
     4261 ttttgattct atttggcagc gtctgacaaa tcatgaagtc acaccgcaac agcatcccat
     4321 tggttttgtt ttaggtgggc aacccggagc aggtaaatca tctctgattg aattagccaa
     4381 aagagagaca aaagataata taatgattat taacggtgat gattttcgat ttctacaccc
     4441 tgattttaat tatatctacc aaaattatgg ggatgatttt gttacccata cagcgaaatt
     4501 ttccggcgaa acggtcgaac gtgcaattga gagagcgata gtcagtaaac tcaacattgt
     4561 tgtcgaagga acatttcgta atgccgcgac gccgttacaa acattaaaaa aactcaaaga
     4621 tgcaggctac caaacagaag tcatgataaa aacaacctca gcggcattaa gctgggaaag
     4681 caccaatgaa cggtatagta aggacaaaga ggcgggaaat attgcccgaa aagtggataa
     4741 aaaccaccat gatatcgtca ccggtttgct ggcagaaaat gcccgcaagg tgtttgcttc
     4801 gaacctgtca gataagtttg cggtgtattc gcgtgagaag atgatattta gttcacaagc
     4861 ggcaacgaat gacgatattg caacgcttat acagaatgaa atcagcggga atacacaata
     4921 ataataagag ctgatggcgt gaaataacgc cgggctttat gcccggcgca ttaccgtctg
     4981 gtacattaag ctatccagtt ccttaacgtc cccttctctg ctatccggta acagcggtgg
     5041 aatttcccca cgcgccagtt ccgcacgaat aaacgcatgt aacaatggac ggcgcggacc
     5101 atattccgct tctcctgcgc gctgtttacg ttccagtaat tcgtctattt ctgcacgtaa
     5161 cggcgcatca agttcgcttc ccgccagtaa ctcagcaaag cgcattggcg gtacgccttt
     5221 acccgcttcg acccagcgca cggccagcag cggacgcagc acgtaaaagt attttttcag
     5281 tcgaacttcg tcgccctgca aatatccggg gaagtttttc tgcgccatcg aatagtaatg
     5341 ccagcgcgca cgcagcggag aaaaccacat tgggaccatt gccttaaacg ccgttatcgt
     5401 ttcttcatct tgttgataaa ccaccggaga atccagccat tctatcagcg tcgggttcgc
     5461 gcctttcaat aatcccagcg ccttacgcca ctcccaaccg gaaacatcta actcgtcgtc
     5521 gatgggcagc tcaataacat cgcgcggtga ctctactcgc agataccatt ccagcgggtg
     5581 aacatacaaa aaccgtacat cgtagtcgct gtcgggcgag gcaaatcccc agccgcggct
     5641 ccctgattca caggcgtaca gtactttcac atcataccgc cgttcaattt cttttaactg
     5701 ctgcgatacc ctttctcgca tcgcagcact gactccattt aatggcatgt tactcaccct
     5761 ttgatattaa ccaacccatg cagaacctga tccagaccgc caaatacaga gatgttatcg
     5821 atccgttcag ccactctctc cagcgtttcc agttctttta agcgtaacgc taccgggttg
     5881 ttttccatga ctttagcggt attgagtaac gaacgcgtcg ctgccgtttc ttcacgtcgg
     5941 cgaatcacat tggcctgcgc ggatttctcc gcctcaacca gctgcgcaag gatatttttc
     6001 atatcgccag gcaggacgat atctttcacc cctaacgagg caatctccat accaaatggc
     6061 aacatgcgcg atttcacctg ttcgctgaca acgtcatcaa tgacctgttt atcttccagc
     6121 aattcatcca acgtacgcgt acctaccgct tcacgcaggg caaattgtaa ttcccgatac
     6181 aaatgatcaa tcggcttcgt taactggcta aatgccaata acacgtcgct gtagcgccag
     6241 ttagcggcga ggttaatccg cagattgacc ttatctttcg ttaagatttc ttgcccgctg
     6301 acttccagta cctgcaagcg ggtgtcaacc acttcagcgt caaccagatg gttaattttc
     6361 cagtatgcgg tcagccccgg cggtaacagc gcctgcgttt caccatcgat tttcagcacg
     6421 cctacatgcc aggccggaac ctgcaccgtt agtattgcat cgcggccttt aactgcccct
     6481 ttgcgccgtg gttgcaatac cgcattcatc acctccgttt gtacctggac ttgctgcgta
     6541 ttcatccgca ccagtgtcaa atcatcctct acacgccagt acaaacggcg cgttgatggc
     6601 ggcaggatct cctgcaaaat accgtccata tacaacgccc cggcttcaat ctccgacaag
     6661 tctgccacca ggcaatattt ctccacccaa tcaggctgaa agcggcgcaa ataatccgcc
     6721 agcacgtcgg gaacttcgct gccatcgagc gtaatcagca agacttctgg cgtatttaac
     6781 cagggtaaaa gatgctcgcc tgcatgtagc actttgtagt aatcgccgtt tctggagagt
     6841 aatgccagct gatcttttct tacgcttatt tttttaatca ttatctattc cttgcttaac
     6901 agccgtcgcg gaagcagaca cgaagcgaca ggggaattcc cttttgcctg ttacccggca
     6961 tctttatcgg cgacgttatc ggggatcgct gactgcgcaa tccctcactt tttatttaat
     7021 aagttaaaac cagtattcat ttcaggaatg aatgtgggaa tcgaacccat aattggcgtc
     7081 aaaggctgac ggaacacgcc cggaaaacag cctggccgtt cctggtgtgc cgacggggtt
     7141 tgaccccctt tgtaattcag tttcaacgcg tacgagatat t
//
LOCUS       DAZFKM010000198         7142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFKM010000198 DAZFKM010000000
VERSION     DAZFKM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(279..887)
                     /locus_tag="ACHKAY_004743"
     CDS             complement(279..887)
                     /locus_tag="ACHKAY_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO5703270.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1313..1381)
                     /locus_tag="ACHKAY_004744"
                     /pseudo
     CDS             complement(1313..1381)
                     /locus_tag="ACHKAY_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1640..1987
                     /locus_tag="ACHKAY_004745"
     CDS             1640..1987
                     /locus_tag="ACHKAY_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703271.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2192..2893)
                     /locus_tag="ACHKAY_004746"
     CDS             complement(2192..2893)
                     /locus_tag="ACHKAY_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO5703272.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2890..3909)
                     /locus_tag="ACHKAY_004747"
     CDS             complement(2890..3909)
                     /locus_tag="ACHKAY_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5703273.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3906..4445)
                     /locus_tag="ACHKAY_004748"
     CDS             complement(3906..4445)
                     /locus_tag="ACHKAY_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO5703274.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(4515..4745)
                     /locus_tag="ACHKAY_004749"
     CDS             complement(4515..4745)
                     /locus_tag="ACHKAY_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703275.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4850..5539
                     /locus_tag="ACHKAY_004750"
     CDS             4850..5539
                     /locus_tag="ACHKAY_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5703276.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            5621..5884
                     /locus_tag="ACHKAY_004751"
     CDS             5621..5884
                     /locus_tag="ACHKAY_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703277.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            5897..6340
                     /locus_tag="ACHKAY_004752"
     CDS             5897..6340
                     /locus_tag="ACHKAY_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703278.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            6891..7097
                     /locus_tag="ACHKAY_004753"
     CDS             6891..7097
                     /locus_tag="ACHKAY_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO5703279.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
BASE COUNT         1895 a         1681 c         1587 g         1979 t
ORIGIN      
        1 ctaattttta tgattatacc cttatcaggt atgatgtagg aactgaaaac aatattggat
       61 atcattttcg gtatttctct gcaaattcca gcccacagga tctcggtgta caatctgtcg
      121 ctgatcgcgc aaatttaggg tcaatcactt cattttttgc atcaaaccct cactaacatc
      181 gaacgattat agtacaagta gtgttcttga cttctcatta aaccgtcagc attcaattaa
      241 ttacaaaata ctagcggcat ttctctcccg caactttatt acagccgttc agaacgtaaa
      301 tgcgtcaata ctcgactcca cttatcatcc tgccacggcc ggaattttac atgtgccgtt
      361 tctctggcaa ggattgcctg tgccttattg agtatccggg gatattcttg ttcgattgaa
      421 gtgaagcgac cggcttcgcg gtgttctgct acctgaagaa gcggagtgac gttttggcaa
      481 gcggttaaca ttgtgtcact tgcccgccat aaccaagcaa gtgtgcaaag ttcgttatca
      541 gtgaattgtt ttgtgattgg ggattgttga atttctcgat cgagaatatc cagcacccag
      601 cggcggaatt ctttggccac aggagtgcgg gcgaacatgg cgatcagatg ggcgccacgg
      661 agggagaaaa tgcgagtctt cttacgataa tttcctgagg tcgttgattc gacgacctga
      721 gacattccgt cagtaaactc atcaatattt ttgttgtaga tgtcggttac tgctttggtg
      781 ctggcatatt ttaatgctgc cgccaattct ttgctggtaa accagatctg gttgttgtga
      841 tttataggag tgaattttac atcgcggaat gcgagctgag tgaccatagc gataatctcg
      901 ttttgaattt gagctatcac caccgacgac gccaatcgac tggtggtgga ctgtgcaagg
      961 ttggcgtaac cggtcaaaac gaaatccggc gcttccgaag aagccctcac acagcccacc
     1021 ataatttttg tgtagacgtg cactgcgcat aaaaaaaccg cgaacgcggt tatgcgtcgt
     1081 tttgatgtcc gggacgccaa tcccgtgtgc cgattttgcg gcaacgcaca gaatatagcg
     1141 ccggataaat aatgtcgtca acccttgcat atggggggcg cgatgatcac tactaccacc
     1201 ataatcgctc caactgttgc aactacttta ggctaagaca tcacgatttc catatcacct
     1261 ttagccgcca gacaaacaga gactccctgt tcttggctgc caaaaacacc gaatgcaaaa
     1321 aatcgcaggt gtatttctgc atctgtggag ggagaagaga gtttggattg tatgtgcgca
     1381 taaacgtccc cgtttagcgc agaagtcacc gtagttgttc atattctgat gacatgataa
     1441 tttcctattg aatagaaaaa attaacacga ctcattctta tgactggaaa aagcaatcat
     1501 gctaataatc attgttgttc cagttccaaa tgcagcgaaa taatcaccaa tattttttct
     1561 accggtttcg actttgatgc tatattctgc taacacctaa ctaacactac gtttcacaca
     1621 gcaattacat ggaaataaca tgactatcaa agcgactgac atagagattc ttcatcgtta
     1681 tgctgaaggg gtaatggaac gttctaacca tcatgcaaaa aatgttggtg cagccgctct
     1741 tacattgcta ggaggtgtta tctggaaagc cctgcccgga tcaatcgaga taagaacgta
     1801 taacggaagt cttgcaaata tggtgtggtg gcaaagcgaa agaacattaa aaaattatgc
     1861 aatctcatac aaccataatt catgtgaaat tgaaatgaga gatgaatcag tcaaaggtgc
     1921 tgtattattt agcatttcca acgaaaccac tccagaaaaa atattatcac agctttctga
     1981 gctttaatta aaggcggtgc taacaccgcc cataccatta ctccacatct tacgcaactt
     2041 gataattatg ctctattaaa tacccacaat atttcatcaa tgcatgggtc agctacaaaa
     2101 tgtttaccgt ggtaattcct gttttcgcaa catctcaaaa gaacaataaa cactgtattt
     2161 cttcaagtaa cgaagaatct ttctttgccc gtcatacact tgctcctttc agcccaaact
     2221 tagctttgat ttctgcgatc ttcgccagag cctgtgcacg atttagaggt ctaccgccca
     2281 taacaggaag ttgttttact ggttcaggta tcgtctcacc acggttaatt cgcgctgtca
     2341 tacaggtcag ttcatcggca gccttgcgcc gtaattccgc gtcagtcagc gcattggccc
     2401 gcatgttctg gtacaggttg gtaaccaacc agtaatgcgc gttcgatttc cacggataag
     2461 actctgcatc cggatacagg ccacgcttcc ggcaatactc gtaaaccata tcaaccagct
     2521 cgctgacgtt tggcagcccg gcgttaacgg atgcttcttc ccggcaccat gcaacaaact
     2581 gcccgggtga tggcagaaat ggtctattct gccgacgggc aacacgcatt ccggcgctca
     2641 cctgttccat cgtggtgatc ccgttttccc ggaaagccag aacccactgg cggcggattt
     2701 cgttcagttc gttctggtca cgattagcca ggctcgccgg gaaagttgcc agtaactggc
     2761 tgaatacacc gttgattatc tgcgctacct gctgtacctg cggcttttcg tcgtactgtt
     2821 ccggcatgtt gttggcgatc cgacgcatct gctcacggtc aaagttaatc atctgtgcgg
     2881 cgatgttttt catagatcca ccccgtaaat ccagtctgtg tttgtcaggt cgagttttgg
     2941 tttgccgact gtcacgccag cctgttgctt gttccggttg atttcgagct gggtccactt
     3001 gtcgcggagt ttggccggac tcagcacgtt accggaccag aagttgtcct ggcaggccca
     3061 gcggaacagt acacacatgt cgcggtggtt acgtccatca cgttcacgca tcagacggat
     3121 atcgttagcc caccctgcaa aattcggttt tctggctgat ggcgcgatgg tcttcaccat
     3181 gtcaaacatc cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc tgccgctctg
     3241 aatcgcagca tccgctttca ccacaggaag gtcgttttct ggcaggtcag aggattcgcc
     3301 agaattctcg gacgaataag gttttatatt gtcttttgtt agtttgtctt ttgtgtttac
     3361 ctgattcggg taagtgcctt tacctgattt gggtaaactt ttcttacctg attcaggtaa
     3421 atttacctct ttcaggtaaa ctttattttt cttacctgat tcgggtaatg ttgaccattc
     3481 actgaccaca ttattaatgc cgatattccg cccgctctga ataaaaatcc cacgctttac
     3541 cagaacactt tttgcagcag aacacttgtg cggcaatatc ccggtcaact cggaaagttg
     3601 ctcgttgctc acccagtcca gttttttatt aaagccatat gttttgcgca tgacagccag
     3661 gaagaccaga agctggtgct gtgttaatcc ggccagcatt acagcttcca gcaactcatt
     3721 tgcaatgcgc gtataaccat catcgagatc tgccacgcgc ggctcctttt gtgccgcatc
     3781 cggcactgga aaattgaata tctcagcagt gtttgccata attcctcccg caatgagtgt
     3841 gttacgattt gcacctgaaa gtcggttctg ttccagcaga ccggctttcg ccatttctga
     3901 acctgtcata tcgcccccag catggtagta accatcgcca tcaatggacc agccagctct
     3961 gggtccacac gaaacatcga cacaatacct tcactaattt ccttcagttt ctggtggcgt
     4021 ggtgcgttga gaatgacagc ctgttttgcc tcactgagtt ccttttccat ttcagccaac
     4081 ctagccatga agctatcctg ctcaaccagg taaccgcgat attccagcgg tagtaccgcc
     4141 agaattgccg gggtcagttc acgcacgtta tttcggtatt tttcagaatc gaatttgtta
     4201 tcgaggaagc ggaacagctt ctggcgtgta cggctgacat catcagggaa atcgatggtg
     4261 ccgccgccct gctcccgata ctcattcaca atgagtgtgg caacgacatc ctgattatct
     4321 acagccgacc aggcgcggac ggcatcacgg attttttcgt ggcctggcac ctgttttgtt
     4381 tgagaacgat ttatcaccgc agtcgggcta aatccgctag tctgttggta tggaagtggt
     4441 tgcataattg actcctttag tttgaattga ctgttaagtt gattgcttat tgttaaagag
     4501 cgtgaaatgg aaatttaagc tgcgttcttt tcggtgtgtg gaaacaactt cggaagatcc
     4561 gggcgaatct ggtatgcctt cactactcca ccagtagccg taacaatgct gccgacatgt
     4621 tcaggggata cctttgcttt gttgtgaagc cacttataga cggcctgctg tgaaacttcg
     4681 caggcagcgc ccagtttctt ttgtgaacca acgatattga tcgctgtttt gatagctggg
     4741 ttcataacaa cctccgtggt taatttgaat caagattaaa actatggttg tttttagtca
     4801 acaaccattt tcgtttgatg gaataaaacc ttggttgtac atttggacta tgaaaacaac
     4861 actctcagaa agacttaaag aagccagatt agcgcgaggc cttacacaaa aggcgcttgg
     4921 ggatttggtc ggggttagcc aggctgctat tcagaaaatc gaaacaggga aagctaatca
     4981 aacaactaaa atcgtggaga tcgcgaacgc tttgggtgtg cgcgcagaat ggttatcttc
     5041 tggcgttgga aatatgtcag acagtacagt gcaaccaata caatcaactg tcagccattc
     5101 caaatacttc aagattgacg ttcttgatat agaagtcagt gctgggccgg gagtcatcaa
     5161 ccgtgagttt gtagaagttc tacgctcggt tgagtactcg tttgacgatg ctcgtcacat
     5221 gttcgatggt aggaaggcag aaaatatccg catcattaac gtgcgtggtg acagcatgtc
     5281 aggaacgatc gaaccaggtg atctgctgtt cgttgatatc acggttaaat ctttcgacgg
     5341 tgatggtatc tatgcatttc tgtacgacga caccgcccat gtaaagcgcc tgcaaatgat
     5401 gaaggataaa ctgctggtta tctctgataa caagagctac tcaccgtggg accctatcga
     5461 gaaagacgag atgaaccggg tattcatatt cggtaaggtt attgggagca tgccgcagac
     5521 gtacagaaaa cacggataat cagccgcgtg ttgatgaggc ttttgggtaa tacgctgaag
     5581 aaaatacctt ataaaaaata acattacggg aaaggcaaaa atgagtaata aaacacttgt
     5641 taaaaaatca aataactgtg tcgatgcata cgccgacgcg tttggctact cttcgtttgg
     5701 tgacggctct cagcgcctag gttcaatttc tttttttcat aatgtaactg aatggcctgt
     5761 tgatgaaagt gacgatgttg agaacataaa gtacaacatt gcaaccatta gaatgcccga
     5821 agaattgatg cttaaattgg ctgacttcat tcgcgaccag catgacaaag ctaaaacaaa
     5881 ctaatcgtaa ttaattatga aacacgatta ttacgaagct ccaaacctaa atgataaaat
     5941 tgcaaaggcg gcagaatcat tttgtttgca ctccagtgcg tcttcccagc tagcaggtat
     6001 atctggaaaa agatgggaca tgctacaatc aagtggtagc agtgggtctg ggagtgataa
     6061 catgcctgaa aaagttgcaa aactggagtc cgatgttgct catatcaaac gagacgttga
     6121 tgaacttaaa acagacgtta aagctatcga caaaaatatg ataactattc ttgcacgtct
     6181 ggactctata aaagaatcct taacaaacaa gccatcaagt gatgctgtcg atagaaagat
     6241 ttcagacgca aagcttgcgg tattgcttgg tgttccagca atcatcgctg caggaacagg
     6301 actttataag ctatcaatgt actttttctt tagtgcttaa gaaaatgctc gcacccttgc
     6361 attaggtaaa gcgatttttc attccccatt accctcgctc ttaaccaatc atctaaaccc
     6421 ggccaccgtg ccgggttttc ttttgccctc ccctcatcat acaccgttca aaaaaaccac
     6481 cacgacctcg cttcagttat cgctatgcga tgcaagtcac aaaataaatc catcttaaat
     6541 acaaccagtt atatctaaaa caaccaacaa aacaactttt gttgttgacg ataaaacaac
     6601 tatagtttta aatgagttca tcgcaacaac acaacgatac ggcaatcacc tgattcaccg
     6661 ttgcgatgac cgcttagatc cgcagcttga atttcagcag gcttcgggga gtgcgaggga
     6721 tgaaacggac gcgtgaacgt cggtgtgacc agctgaaatc aactcaacat ttcatacctt
     6781 agtcgcttca acgaggcggc ttagttatga caaccggcgg ccatccaccg cctgaatacg
     6841 cgcagaagtc tttatatgtt cagcagccca gcttacgggc aggagttttt atggttcatc
     6901 aacattacgg aacgcagacc gttaatcgcg gtgcggtcat gccaggaatg ctggtcaaac
     6961 gcaaagatgg tacctggact gcatcagcta atttacgcgg acgactttat ctgcatcgcg
     7021 gcattgagcg cacttatacc cgtgacttgc tcgtggaagt ttttctcgac ggacgcggca
     7081 acgctctgaa tcactaatcc cctttcctgt tttccgaatc agcctggcat tccgcgggcg
     7141 at
//
LOCUS       DAZFKM010000199         7111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFKM010000199 DAZFKM010000000
VERSION     DAZFKM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..1445
                     /gene="adeQ"
                     /locus_tag="ACHKAY_004754"
                     /gene_synonym="yicO"
     CDS             111..1445
                     /gene="adeQ"
                     /locus_tag="ACHKAY_004754"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO5703280.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1498..1950
                     /gene="yicN"
                     /locus_tag="ACHKAY_004755"
     CDS             1498..1950
                     /gene="yicN"
                     /locus_tag="ACHKAY_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO5703281.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2161..3351
                     /gene="nepI"
                     /locus_tag="ACHKAY_004756"
     CDS             2161..3351
                     /gene="nepI"
                     /locus_tag="ACHKAY_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO5703282.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3392..3685)
                     /gene="yicS"
                     /locus_tag="ACHKAY_004757"
     CDS             complement(3392..3685)
                     /gene="yicS"
                     /locus_tag="ACHKAY_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO5703283.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            3907..4725
                     /gene="nlpA"
                     /locus_tag="ACHKAY_004758"
     CDS             3907..4725
                     /gene="nlpA"
                     /locus_tag="ACHKAY_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO5703284.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4729..5652)
                     /gene="yicL"
                     /locus_tag="ACHKAY_004759"
     CDS             complement(4729..5652)
                     /gene="yicL"
                     /locus_tag="ACHKAY_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO5703285.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5763..6947)
                     /gene="setC"
                     /locus_tag="ACHKAY_004760"
     CDS             complement(5763..6947)
                     /gene="setC"
                     /locus_tag="ACHKAY_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO5703286.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1791 a         1595 c         1791 g         1934 t
ORIGIN      
        1 ttaaagaata tccattaatg tgcaattgaa atgtgataat tatcactgaa ccgttgttgt
       61 catttgtttt acaaaagcaa gggattaaat ctcctccaat ggacaaaaaa atgaataatg
      121 acaataccga ttacgtgagt aatgaatcag ggacgctttc gcgattattt aaactacctc
      181 agcatgggac caccgtccgc acagaattga ttgcggggat gaccactttt ttaaccatgg
      241 tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc acaaatggac ccgaaagtgg
      301 tgtttgttac cacctgtttg attgccggta tcggcagtat tgcgatgggg atatttgcta
      361 acttacccgt ggcgctggct ccggcaatgg ggctgaacgc cttctttgcc ttcgtggtcg
      421 tgggggcgat gggcatctcc tggcagaccg ggatgggcgc aatattctgg ggcgcagttg
      481 gactattttt gctcacgctg tttcgtatcc ggtactggat gatctccaac attcccttaa
      541 gtttacgtat tggtatcacc agcggaattg gattatttat cgccttaatg ggattaaaaa
      601 atactggcgt tattgtcgcc aataaagaca cgctggtgat gattggcgat ttaagttctc
      661 acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca tcacgtcatt
      721 ttcatgccgc ggtgctggtt tctattgtgg tgacgtcttg ctgtggatta tttttcggtg
      781 atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc attggtgaag
      841 tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc tttatgctga
      901 tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagcg ggcttaatag
      961 atagtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc gtcagttcgg
     1021 tggcgggggc gtttatcggc acctcgtctg ttactgccta tattgaaagt acttctggtg
     1081 tggcagtcgg tggtcgcacg gggctgactg cggttgtggt tggcgttatg ttcctgttag
     1141 ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc ggagcgttaa
     1201 tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat gattttaccg
     1261 aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg atcaccgaag
     1321 ggattgcact cggctttatg tcgtactgca tcatgaaagt atgcaccggg cgctggcgcg
     1381 atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt attctggtgg
     1441 attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg agaactcatg
     1501 atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg ggtgctgaca
     1561 tccggggcca gaaaagcgat tcgccgtctc agcgatcggc tgaacatcga tgtcgtaccc
     1621 gtggagtcga tggtcgatca aatgggaaag tcagctggtg acgaattttt acgttatttg
     1681 catcgtccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat ctggcaaatt
     1741 gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt acaaaaagct
     1801 cgcctcgccg cgccgattac cgacgctcag gtcaggctgg cgctaggttt tctgcgcgaa
     1861 accgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc gttctttcag
     1921 cctgccgagg gcgttcactg gttgcattga aaccgtttcg tcttatcagc ctacagaccg
     1981 cgcctgacct gtcgggtgga taagacgttc acgccgcatc cggtaatctt tgccctgttc
     2041 cgtttcgatc ttaactccag ctcaagcaac accgtaaaag ttgcaaaaac gttaaatgcg
     2101 tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac gggtaacacc
     2161 atgagtgaat ttattgccga aaaccgcggc gcggatgcca tcacccgacc gaactggtca
     2221 gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta tcgttgagtt tttgcccgtc
     2281 agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc cgggcaatcg
     2341 gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca gacaattcag
     2401 gctactgacc gccgctacgt tgttattttg tttgccgttt tgctgacgct ctcctgcttg
     2461 ctggtttcct ttgctaactc attcagtttg cttttaatcg gtcgtgcctg tctggggctg
     2521 gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt gccgccgcgt
     2581 acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct ggtgattgcc
     2641 gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt taatgcggcg
     2701 gcggtgatgg gcgtgctgtg tattttctgg attatcaaat cattgccttc actgccaggc
     2761 gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg tgtgatggca
     2821 gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac atatattcgc
     2881 ccggtgtata tgaacctggc gggattcagc gtggatggct taacgctggt gctgttgagt
     2941 tttggcattg ccagctttat cggtacgtcg ctttcgtcat tcattcttaa acgttcggta
     3001 aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt gctgacgttg
     3061 tggggaagcg ataaaatcgt tgctaccggc gtggcgatta tctgggggct aacttttgca
     3121 ttggttcccg tcggctggtc aacgtggatc acccgctcgc tggccgatca ggcagaaaaa
     3181 gccgggtcta ttcaggtggc ggttattcag cttgctaata cctgtggcgc ggcaatcggc
     3241 ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg cacattgatg
     3301 ttgctgactg cattgttggt tactgcaaag gtgaaaatga agaaatcctg atatcaggta
     3361 aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc cgggcattct accttaccca
     3421 acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc tcgtgtatat
     3481 gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct gataatgacg
     3541 cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt ggttttctct tttgcggact
     3601 gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg catagcgaaa aaagtgaaaa
     3661 taagcagtag tgtcgttggt ttcatggatt caacctttta caaatgtgta tcgaatgaag
     3721 cataagtgta gtttgctttc atcgctctca tcatgctgta cgaaacgcgc ttgcgtggtc
     3781 agtaagaagt gccagacttt atattccact tttattcctt tttattctta tcgatagcgt
     3841 ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag gtaatcatta ataaaaggat
     3901 aaaaaaatga aactgacaac acatcatcta cggacagggg ccgcattatt gctggccgga
     3961 attctgctgg caggttgcga ccagagtagc agcgatgcaa aacacattaa agttggcgtt
     4021 ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtggcaaa agagaaatat
     4081 ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga tgcgacaaat
     4141 catggcgaac ttgatgcgaa tgtcttccag catcgaccct ttcttgaaca agataatcag
     4201 gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccctat ggcgggttat
     4261 tccaaaaaaa tcaaaaccgt ggcgcaaata aaagaaggcg cgacagtggc gatccccaat
     4321 gacccgacca accttggtcg cgcactttta ctgttgcaaa aagagaagct aattactctg
     4381 aaagagggaa aagggttatt acctaccgcg ctggatataa ccgataatcc tcgtcatttg
     4441 cagattatgg aactcgaggg ggcgcagctc ccgcgcgtac tggatgatcc caaagttgat
     4501 gtagcgatta tcagcaccac ttacattcag cagacagggc tttctccggt gcacgacagc
     4561 gtatttattg aagataagaa ttcgccgtat gtgaatattt tggtggcacg ggaagataat
     4621 aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga agtcgctaaa
     4681 gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct ggtaattctc actcatgccg
     4741 cgccggacga tttatttttc tggcgcggcg acgggaatcc attgaaatca aaattaccga
     4801 tgacagaatt agcagcgttc ccagccagtc cggtaatgtg aacgtgatcc ccagcaacag
     4861 caaagagagt agcgcgctac ttaatggttc tgcacagctc aaaatgctgg cttttggacc
     4921 gccaattaat tgtgctcctt tcaggtacag actaaatgtc agggacgtac caatgacgat
     4981 caaataaaaa aacgccagaa tcaaactgcc gttaacgaca aagtttgttc cttgtctggc
     5041 ataaaaaggc aacagaatca gaccgccaat cagcatactc cagccgacga ctggtaacgt
     5101 gccatagcgg gcaattagtg ttgagggata ggtggtatag aatgcagcag caaatgccga
     5161 ggcaatgccc cagaacaacg cggcaggaga gatcgataat gacgtcggat taccgtgtgt
     5221 caccaataaa aaagtcccga ccagcgatgt caaaatagcg cagaaaacca gaatgcccgg
     5281 gcgcgattta cgcaccagtg agaaccaggc gacgataatc gtcggtgaga ggaattgcag
     5341 caccgttgcc gtggctgcgt tcgatttttc gatggttagc aaaaaagtga gctgtacagt
     5401 tagcgcgcca accacggaaa aaatcagcag gctaatggca tctttatgat tgttaataat
     5461 agaaaagatt ttatcgccat gaacaaatga cagcgtcagt agaatcaaac cggcgaatat
     5521 caaacgcgtc atagtcaaaa actgcgacga catctggctt tgctccatga tgtattgcgc
     5581 acagacccct gaacttcccc acaacacggc ggcaatcaga acgttcagca tcccctttct
     5641 ggtggaaccc atttttccct ctgcatactg ttttgtcgtt tatgtatgca ggcatgatag
     5701 caaaaatggc gaggatggct ctgtttgcgc cacgccgatt tttcttagcc cagactggtg
     5761 agctaaatat ctttaataaa cagcaggcaa atcatcgcaa tacccaacat ccctatcgcc
     5821 agccagaaca acgcatggta gctccagatt tcaaccataa ttccgtcaac agagccggcg
     5881 ataatccagc cgacgcgtga agtatttgca tataacgttg tcgcagagcc tattttttca
     5941 ggcatcaagt cctgaaaata aagcatgccg ataccacaga gaataccaag gaagatggca
     6001 ttaagaattt gcaattccag ctcaaccgcc ggagtcgtcg ccatgagtac gctggcgtaa
     6061 aaacacattc cactcacgat agcaatgagc attaatagtc gcttgccaat acgtttcatg
     6121 taatagcctg cgattaacat catcggaatt tccagaccgg cagcgataca aatcatttct
     6181 ccagccagtt tatcggttag atgcagttca tcaataataa atagcggcat atttatcatg
     6241 tagagattat tcgccgccca catcattgaa cagaccacaa aaagtagccg cgtatcccgc
     6301 ttcctgtggg tggagggtaa aatttctacg ggttgggtaa cgacaggaat atttctttgt
     6361 attgatggca aaaacaacca gactatcagt ccgcaaacaa caaatgcgat ggcagcggtg
     6421 agatacatca ctttaaaact aaatcccatt gccagttcat aagcgagcgg tggcccgata
     6481 acccaggcaa gcgagatctg agcacgtaaa aatgtactga acatgaccgt ctcacggcct
     6541 gttctgtcgg cgtgttcacg ggcgagggcg aacatttgcg ggtttgcggt ggaagcaaaa
     6601 ctactcagaa gtacgcccgt tgagaggaga atgaagtagt tgcgattcca cgcaaaaagc
     6661 gtgcaggcca gcactccaaa taagcaacat agcagaatca gtaatttacg gtcgccttgt
     6721 ttatcggagt gccttgccag aaattgactg accagaattc ccataatagc gctaccggtg
     6781 aagaaaaaat ctaccattat aggacgggct ttcagttcat ctccgaggaa tatacttagg
     6841 gtaggagtct gaagagcgcc cgcaataccc gtcagaaagg cgacaagtaa aaatgccgcg
     6901 gcagtgagat caagtatttt tgatggagtg gtagccgttt tttgcattct tctgaatcac
     6961 cgggcgacaa aatataactg agttacaaat aagtaccagt cgaatgagta ttgtattaag
     7021 aatttttgag tatcgctgat acaaccagaa agcaatgcta gcgcaatatt atagggccag
     7081 caattgagat tattagcaaa agcatgttaa g
//
LOCUS       DAZFKM010000200         7058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFKM010000200 DAZFKM010000000
VERSION     DAZFKM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            564..1553
                     /gene="araF"
                     /locus_tag="ACHKAY_004761"
     CDS             564..1553
                     /gene="araF"
                     /locus_tag="ACHKAY_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO5703287.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1623..3137
                     /gene="araG"
                     /locus_tag="ACHKAY_004762"
     CDS             1623..3137
                     /gene="araG"
                     /locus_tag="ACHKAY_004762"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO5703288.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3152..4138
                     /gene="araH"
                     /locus_tag="ACHKAY_004763"
     CDS             3152..4138
                     /gene="araH"
                     /locus_tag="ACHKAY_004763"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO5703289.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4305..5105
                     /gene="otsB"
                     /locus_tag="ACHKAY_004764"
     CDS             4305..5105
                     /gene="otsB"
                     /locus_tag="ACHKAY_004764"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO5703290.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5080..6504
                     /gene="otsA"
                     /locus_tag="ACHKAY_004765"
     CDS             5080..6504
                     /gene="otsA"
                     /locus_tag="ACHKAY_004765"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO5703291.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6511..6939)
                     /gene="uspC"
                     /locus_tag="ACHKAY_004766"
     CDS             complement(6511..6939)
                     /gene="uspC"
                     /locus_tag="ACHKAY_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO5703292.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1786 a         1637 c         1829 g         1806 t
ORIGIN      
        1 atgatagcgc tttttgtatc ttgttgatat taatcaatga atttgaattc atgatgaagt
       61 gatgatttta taaaacgttt tttcattttt gcgagatggc tctcattata cgtgttctgt
      121 taattaatga gtacagtgat aattaatggc gaaatgaatg acgtgcattt tccacatctt
      181 tgagttgcgg ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact
      241 ggttaaaaag aagacatccc gcatgggtac caaagacaac aaggatttcc aggctaatct
      301 tatggattaa tctgctgtgc attcgacaat ttgtctgaca aattggcttt cccttatgtc
      361 ttttcccgct aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct
      421 atggagaatt aatttctcgc taaaactatg tcaacacagt cacttatctt ttagttaaaa
      481 ggtaatgctt tgttttccga ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa
      541 cgacactaaa gctggagaga accatgcaca aatttactaa agccctggca gccattggtc
      601 tggcagccgt tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga
      661 agcaaccgga agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg
      721 atttagggtt tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga
      781 tcgacagcct ggctgccagt ggcgcaaaag gtttcgttat ttgtactccg gaccccaaac
      841 tcggctctgc catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gccgtggatg
      901 accagtttgt taacgccaaa ggtaagccaa tggataccgt tccgctggtg atgatggcgg
      961 cgactaaaat tggcgaacgt cagggccagg aactgtataa agagatgcag aaacgtggct
     1021 gggatgtcaa agaaagcgcg gtgatggcga ttaccgccaa cgaactggat accgcccgcc
     1081 gccgtactac gggatctatg gatgcgctga aagcggccgg attcccggaa aaacaaattt
     1141 atcaggtacc taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc
     1201 tggttcaaca tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtgc
     1261 tgggcggcgt acgcgcgacg gaaggtcagg gctttaaagc ggccgatatc atcggcattg
     1321 gcattaacgg tgtggatgcg gtgagcgaac tgtctaaagc acaggcaacc ggcttctacg
     1381 gttccctgct gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact
     1441 gggtagcaaa agacgttgaa ccgccaaaat ttaccgaagt taccgacgtg gtactgatca
     1501 cgcgtgacaa ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg
     1561 gaaaaattcc cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg
     1621 ttatgcaaca gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg
     1681 ttaaggcgct gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg
     1741 gtgaaaatgg cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa
     1801 ccacgggttc tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac
     1861 ttaacgcggg tgtggcgatt atttaccagg aactacatct cgtgccggaa atgaccgtcg
     1921 cggaaaacat ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc
     1981 tgaattatga ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc
     2041 cgctgaaata tctctccatt ggtcagtggc agatggttga aatcgccaag gcgctggcgc
     2101 gtaacgctaa aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg
     2161 acaatctttt ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt
     2221 ctcaccgtat ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac
     2281 gttatgtcaa aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga
     2341 tggtcgggcg cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc
     2401 gcctgcgtct tgatgctgtg aaagcaccag gcgtgcgtac gccaataagt ctggcggttc
     2461 gcagtggtga aattgttggg ctgtttggtc tggtaggggc ggggcgtagc gaattaatga
     2521 aaggcttgtt tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga
     2581 tcgatattcg taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagatc
     2641 gcaaagcgga aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca
     2701 gacgtaaaca tgtgctcggc ggttgtgtaa tcaacaacgg ttgggaagaa aacaatgccg
     2761 atcaccacat tcgttcgctc aacatcaaaa cgccgggcgc tgagcaactg atcatgaatc
     2821 tctcaggcgg aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg
     2881 tcattttgct ggatgaacct acgcgcggca ttgatgttgg tgctaagcat gaaatttaca
     2941 acgtgattta tgcgctggcg gcgcagggtg tggcggtgct gtttgcctcc agcgacttac
     3001 ctgaagtcct cggcgttgcc gaccggattg tggtgatgcg ggaaggtgaa atcgccggtg
     3061 aattgttaca cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca
     3121 gccaggcagt tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg
     3181 cgcacctaag tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt
     3241 gtttgcggtg ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa
     3301 tatgaaaggg ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt
     3361 ctgccttgct tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt
     3421 caccacggcg gtggttatca acctgactga aagcctgtgg attggcgtgg cagcggggtt
     3481 gctgcttggc attctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa
     3541 tgctctgatc acaacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc
     3601 agacggtaaa gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg
     3661 gttcggtctg cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgtt
     3721 gctgaataaa accacctttg gtcgtaacac cctggcgatt ggcgggaacg aagaggctgc
     3781 gcgtctggcg ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct
     3841 ggtatctgcg atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac
     3901 gtcgattggt tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa
     3961 aggtggcatc ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga
     4021 aaacgccatg aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat
     4081 cctgctggca gcggtgatct tcgaccgtta caagcaaaaa gcgaaacgca ctgtctgatg
     4141 cttttttcca caacaattta acgttttttc ccaccacagc cagccgccac aacggttggc
     4201 tgttcttcat tgcaaatggc gacccccgtc acactgtcta tacttacatg tctgtaaagc
     4261 gcgttctgcg caaaacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa
     4321 ccgaaacccc tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg
     4381 cggaaatcaa accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac
     4441 agctactggc aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg
     4501 agcttgacgc actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc
     4561 gccgtgacat caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata
     4621 ttagcgtgca actgcataca gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag
     4681 ggatggcttt tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat
     4741 tagcgcaacg tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg
     4801 tcgagatcaa accgagaggt accagtaaag gtgaggcaat tgcagctttt atgcaggaag
     4861 ctccctttat cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct
     4921 tcgcagtcgt taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg
     4981 catcatggcg actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccaccg
     5041 cattacaaca aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta
     5101 tctaaccgga ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc
     5161 atactggggg cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg
     5221 aatgaggatc agccgctaaa aaaggtgaaa aaaggtaaca ttacgtgggc ctcttttaac
     5281 ctcagcgaac aggaccttga cgaatactac aaccaattct ccaatgccgt tctctggccc
     5341 gcttttcatt atcggctcga tctggtgcaa tttcagcgtc ctgcctggga cggctatcta
     5401 cgcgtaaatg cgttgctggc agataaatta ctgccgctgt tgcaagacga tgacattatc
     5461 tggatccacg attatcacct gttgccattt gcgcatgaat tacgcaaacg gggagtgaat
     5521 aatcgcattg gtttctttct gcatattcct ttcccgacac cggaaatctt caacgcgctg
     5581 ccgacatatg acaccttgct tgaacagctt tgtgattatg atttgctggg tttccagaca
     5641 gaaaacgatc gtctggcgtt cctggattgt ctttctaacc tgacccgcgt cacgacacgt
     5701 agcgcaaaaa gccatacagc ctggggcaaa gcatttcgaa cagaagtcta cccgatcggc
     5761 attgaaccga aagaaatagc caaacaggct gccgggccac tgccgccaaa actggcgcaa
     5821 cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg tcgaacggct ggattattcc
     5881 aaaggtttgc cagagcgttt tctcgcctat gaagcgttgc tggaaaaata tccgcagcat
     5941 catggtaaaa ttcgttatac ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat
     6001 caggatattc gtcatcagct cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa
     6061 ttaggctgga cgccgcttta ttatttgaat cagcattttg accgtaaatt actgatgaaa
     6121 atattccgct actctgacgt gggcttagtg acgccactgc gtgacgggat gaacctggta
     6181 gcaaaagagt atgttgctgc tcaggaccca gccaacccgg gcgttcttgt tctttcgcaa
     6241 tttgcgggag cggcaaacga gttaacgtcg gcgttaattg ttaaccccta cgatcgtgac
     6301 gaagttgcag ctgcgctgga tcgtgcattg actatgtcgc tggcggaacg tatttcccgt
     6361 catgcagaaa tgctggacgt tatcgtgaaa aacgatatta accactggca ggagtgcttc
     6421 attagcgacc taaagcagat agttccgcga agcgcggaaa gccagcagcg cgataaagtt
     6481 gctacctttc caaagcttgc gtaggagcgg ttaatctccc gtaagtggaa ctaacagcac
     6541 atcgacctca cttgaggcaa taactctttt cgccgagcag gatgctcgcg aaaagaaact
     6601 gtgattgtga ttaccgcaaa tcaccaaatc gaaatgatgt ttgtgacata cttccagaat
     6661 atgttcgctt aactcaccgt aggcaataaa cgtcttgtca acgggatacc ccgcatcctg
     6721 aattaactta tcaagaaagc tttgcgtttc ttcttgcatc acgctgcgta aatcttcaag
     6781 catcggcgcg gctaattgat tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat
     6841 gtgtcccttt actggcctgg cgatagatac cgcttttgcc agcagttgct gactttccgg
     6901 tgtaaccgca acagcgacaa gaatattgct atagctcata acctgttcct tattctgtga
     6961 acttcaggtg acattaaagc tttactccta atggagaaac gacgcaatcc caactcggtc
     7021 aaacaggtaa atattagtct gattattagt aaagacaa
//
LOCUS       DAZFKM010000201         6881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFKM010000201 DAZFKM010000000
VERSION     DAZFKM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(81..842)
                     /gene="kduD"
                     /locus_tag="ACHKAY_004767"
     CDS             complement(81..842)
                     /gene="kduD"
                     /locus_tag="ACHKAY_004767"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO5703293.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(872..1708)
                     /gene="kduI"
                     /locus_tag="ACHKAY_004768"
     CDS             complement(872..1708)
                     /gene="kduI"
                     /locus_tag="ACHKAY_004768"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO5703294.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1995..3176)
                     /gene="yqeF"
                     /locus_tag="ACHKAY_004769"
     CDS             complement(1995..3176)
                     /gene="yqeF"
                     /locus_tag="ACHKAY_004769"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO5703295.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3431..4660
                     /gene="yqeG"
                     /locus_tag="ACHKAY_004770"
     CDS             3431..4660
                     /gene="yqeG"
                     /locus_tag="ACHKAY_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO5703296.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5120..5752
                     /gene="yqeH"
                     /locus_tag="ACHKAY_004771"
     CDS             5120..5752
                     /gene="yqeH"
                     /locus_tag="ACHKAY_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703297.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
     gene            6086..>6881
                     /gene="yqeI"
                     /locus_tag="ACHKAY_004772"
     CDS             6086..>6881
                     /gene="yqeI"
                     /locus_tag="ACHKAY_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703298.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYRE"
BASE COUNT         1960 a         1609 c         1426 g         1886 t
ORIGIN      
        1 aaccatgata tggattatga tgatctacag gtataaaaaa ccctgccatg cggcagggtc
       61 ataaaagtaa gaagaatgaa ttaacgcgcc agccaaccgc catccacggc aatggtataa
      121 ccattcacat aatctgaagc gctggaggca aggaacacta tcggccccat caggtcactc
      181 ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt ccgcgctacg ttgttcatct
      241 gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
      301 atgttgtgtt tagcccattc gttcgccatc aatcgcgtca cacccatcac gccgcttttt
      361 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
      421 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
      481 aatacgctct tgatattcag gttcatgaca tcgtcccagt ccttttcgct gaactcgaga
      541 gcatcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaactcc
      601 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
      661 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
      721 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
      781 ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc acttaaaatc
      841 ataacaatac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
      901 ccatatcatc aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
      961 ttccgacgcc ggaatgaatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
     1021 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
     1081 tattgaaata gaaataaact tccatccggc gctcgtgagt gtgacacggc atggtgttcc
     1141 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttggcaggtt tccagcacat
     1201 ccgggacaaa atatttgtta attgtgcgac ggttactggt gaggttatcg cctaacgtga
     1261 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
     1321 aattgtaata aaactttgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
     1381 cacctttacc cacatacagg gcgtcgcggt gaccgatttc atagcattgg ccatcgacag
     1441 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
     1501 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
     1561 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
     1621 atacctcttc aaccaaaaat tcattgcgca gcccttgggt atccagcgtt tttgcgtgcg
     1681 cactgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
     1741 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
     1801 ctagggtttt caattaaaaa taaaacaatg tttcattttt aagttaagaa ttaaaaaaag
     1861 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
     1921 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
     1981 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgccctg gcccccgccg
     2041 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
     2101 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
     2161 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
     2221 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
     2281 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
     2341 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
     2401 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
     2461 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
     2521 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
     2581 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
     2641 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
     2701 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
     2761 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
     2821 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
     2881 gtagccagat gcagtgcttt aagcccagaa ccgcaaacgt cattaatagt gattgcagaa
     2941 acgctattag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
     3001 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
     3061 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
     3121 ccgcgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca
     3181 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
     3241 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
     3301 tggcacgtaa ggtttgcaac cactaaccca ccaatagagg ggtagatagg gcgttaatct
     3361 cccatactta acctggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
     3421 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
     3481 cggcacagcc gttggtgcag gcacgctctt ccttcctatt cagttaggtt cggcaggggc
     3541 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattggc cacataaagc
     3601 cttatgccag ttcatcctct catcgaaaac atcagcaggt gaagggataa cgggcgcagt
     3661 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
     3721 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
     3781 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
     3841 cctgaatctc atttttctga tgggacgcca tgcaaccatt cgggtaatgg gttttttggt
     3901 attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc
     3961 tgatctatta acgacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc
     4021 gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga
     4081 cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc
     4141 ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc
     4201 accttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat
     4261 gctgccgaac gccccagcat ggctgtcaat ttccgggatt attgtcgcag tagttgcgat
     4321 gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacgg aggtcgtcaa
     4381 aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccgcg cactatcaat
     4441 tatgttggta tcgctgatca ccttcattgt ttgttgcatt aacccgaacg cgatttcgat
     4501 gatttacgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgttgtc
     4561 aacgtatctg atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat
     4621 cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctga tatccattgt gaatggttca
     4681 aaattgctac aattttgaac cattccagtt ttcataatgt tcattcgtta ttattccgca
     4741 ctatcacttc cctcatcata tcctagtatt atcagttagt taaacacaca aagagatctg
     4801 aacatcttaa ataagactat ttaagatgca taacttagat tcgcaagata tcttccggtc
     4861 attcaaaaac aatttgcgat atagatattc tcataccatg agatagttat ctgaaccacc
     4921 ctcaaccagg taagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt
     4981 ttaaaatgtc ttaatcagaa ttatagcaaa tacagattaa tcgcaatttt tcccagcaaa
     5041 attacaaaat catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac
     5101 ggatagggag gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag
     5161 ttgctcaatg cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa
     5221 ctcgtgtttt tgtatgtggc ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg
     5281 tatcttaaca aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt
     5341 aagtttttta cgtctattcc ttgaaaaaga ctggattgac tatcttgcaa gtactgacat
     5401 ggggattgtt ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca
     5461 caactcagca atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa
     5521 gatcagacaa ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca
     5581 gatggaattc accataatgg ggtatatggt ttctggttat aatccatatc agattgctga
     5641 agttctggat atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat
     5701 gggtggtaaa ataaacgaat tatttattcg ttcacattcg gtccaatatt gatactcaac
     5761 gaccagccag aatcatactc tgtttatacg ggcatttatt gaaatgtccg tattttataa
     5821 ataggccgtc ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca
     5881 gaagcattac gaaatatgaa tttacaaaca atagtggcat aaatgttaac catgttaatt
     5941 tacgtaaagt tttacgttgc aacattaaag cctcatttca atcatcatga taaatataaa
     6001 attaatatat atttatgccg taaataccaa tatacttagc aaactatgtg atctccattt
     6061 cgattgattt agtgtttatt gacgtatgta ctggattatt aacgataata tcgagttctg
     6121 gcctgagcac cgaaaattaa tatcggtaca taacgccgat cttaacgtcg ttctgacaac
     6181 gccagccagt cgatgtttat cacttctact tgaagctttt cctgatgtgg ttgcacaaca
     6241 agattttttc accagagtct gggaagaaga aggtatgcgt gtgcctacta acacgttata
     6301 tcagaacata tccattatca gacgcggatt tcgcgctgtt ggtgatacta cccactcgct
     6361 aattgcaacc gtgccgagaa gaggattcaa gatccataat gacattaaca tacaaaatca
     6421 tgtaataaac tcgtcaacag acgcacatac acacaatgcc ccacctgcca taaaagttaa
     6481 tgcgggatac aaagagagca ttggtggcgc aaagaatttc aataacaaaa tcctcaaaca
     6541 tataaaaagt catctaatta tgttgagcgc atttgtcata ggcgcatatt ctgcatattg
     6601 gctatggaat aataatcaac cgaagccatt tttcaaggat tacaagactg ttgcggaaat
     6661 taatggctgc cattttaatg taacagaaga tacaattgac gggttgaaag agttcgataa
     6721 atataaaaca cggatactgg attcggggat taactgtaaa aaacatccgt ggttatactt
     6781 ccctcttgca aaatcctcac cggggatgat tgttatggca tgcaataaaa attataacca
     6841 acatgaagtg gcagactgtt taaccctgtc ttaccgtgag g
//
LOCUS       DAZFKM010000202         6878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFKM010000202 DAZFKM010000000
VERSION     DAZFKM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(186..854)
                     /gene="artM"
                     /locus_tag="ACHKAY_004773"
     CDS             complement(186..854)
                     /gene="artM"
                     /locus_tag="ACHKAY_004773"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO5703299.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(854..1570)
                     /gene="artQ"
                     /locus_tag="ACHKAY_004774"
     CDS             complement(854..1570)
                     /gene="artQ"
                     /locus_tag="ACHKAY_004774"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO5703300.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1577..2308)
                     /gene="artI"
                     /locus_tag="ACHKAY_004775"
     CDS             complement(1577..2308)
                     /gene="artI"
                     /locus_tag="ACHKAY_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO5703301.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2326..3054)
                     /gene="artP"
                     /locus_tag="ACHKAY_004776"
     CDS             complement(2326..3054)
                     /gene="artP"
                     /locus_tag="ACHKAY_004776"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO5703302.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3272..3787)
                     /gene="ybjP"
                     /locus_tag="ACHKAY_004777"
     CDS             complement(3272..3787)
                     /gene="ybjP"
                     /locus_tag="ACHKAY_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO5703303.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3913..4236
                     /gene="ybjQ"
                     /locus_tag="ACHKAY_004778"
     CDS             3913..4236
                     /gene="ybjQ"
                     /locus_tag="ACHKAY_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO5703304.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4233..5063
                     /gene="amiD"
                     /locus_tag="ACHKAY_004779"
     CDS             4233..5063
                     /gene="amiD"
                     /locus_tag="ACHKAY_004779"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO5703305.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5060..6073)
                     /gene="ybjS"
                     /locus_tag="ACHKAY_004780"
     CDS             complement(5060..6073)
                     /gene="ybjS"
                     /locus_tag="ACHKAY_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO5703306.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6172..>6878)
                     /locus_tag="ACHKAY_004781"
     CDS             complement(6172..>6878)
                     /locus_tag="ACHKAY_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO5703307.1"
                     /translation="SVWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIA
                     FDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQV
                     VNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPE
                     KQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWLLMIPAHLFIF
                     RGMAKQIARLAEQSTD"
BASE COUNT         1675 a         1802 c         1810 g         1591 t
ORIGIN      
        1 aaatgaaata aaaaaagacg gataccttag tgagttgtcc gtcttcatta taagaattta
       61 tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac cgtgctgatg
      121 tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga atgtacgcag
      181 ctacattaat ttcgccgttc gaatgccagt gctttgcgct cgatcagacg catcatcagc
      241 gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac cattacatcg
      301 taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtaa
      361 gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga gagcgagcgt
      421 ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta
      481 caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta
      541 tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc tgacaacaaa
      601 tgccacagtg ccggatactc ctgcaaagtc ggaaactggc ccggcccgta ataaatcagg
      661 aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg caccagccac
      721 accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc
      781 acaatcagcg aggcaacggt tagcgtcagg ctggtgtgta gccctttcat cagttcgggt
      841 aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt
      901 tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc
      961 aggtaaatgg ttcctgggta cgagtagcga tgctttttgt ttgcagcatt aaatcattca
     1021 cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg ttaccgaggc
     1081 caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg
     1141 acagccccag cgcctgacca gactcccact gacccaccgg caccgctttc aacgcgcccc
     1201 gaagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga
     1261 acgggctaac gtcgaagttc tcaatgtcca tctgcactgg gatctgcacg aacccaagat
     1321 tgatagtgaa gccatccgaa agcgtcagca gcagctgcga ggagccaaaa tagataaaca
     1381 gcaccaccag aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg
     1441 cgacaggacg ccattttgcc gactcccata ccgcaaagaa catcgccagc gccagcccga
     1501 caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct aaaggaaaaa
     1561 attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat
     1621 ctttcttcac tttttccagc gcagtgttga gtttctgctg cagctcagtg ttgccctgac
     1681 gtaccgcgat gccgaggcca gtgccgaagt aatctttatc ggtcactttg tcgcccaccg
     1741 ccgccagttt cgggttatct ttcagccact cagtgaccac tgcggtgtca ccgaagacgc
     1801 cgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca tacggaacgg
     1861 tagtgatttc cgggtgctta tccataatga atttctggtg tgtcgtcccg ttctgtacgc
     1921 cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga cccacaaaca
     1981 gggcagagtt gtcatagtac ggggtggtaa acagcacctg cttttcacgc tccggagtga
     2041 tatccatgcc cgccatcacg gcttctacgc gacggaattt caggcttggg atcaggctgt
     2101 caaacgcctg gttagagaaa gtgcaggttg catcaatctc tttacacagc gcttgtgcca
     2161 ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg
     2221 atgcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa
     2281 ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata
     2341 gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat
     2401 atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg
     2461 ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg
     2521 tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta cctgcggttc
     2581 catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg
     2641 gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag
     2701 cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac
     2761 ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc
     2821 gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag
     2881 cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc
     2941 gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa
     3001 cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac
     3061 tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga cagaataatt
     3121 atgcaatatt tctgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca
     3181 aaataacggg ggcggtggtc ggcgagcata aatgtcggca ttcctcacga aatgccggac
     3241 aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg
     3301 tacccgccgt ggcatgaacg ctgccgccta aatagcgtac atcgtcgatc acccaacact
     3361 ggccttcctg aatcattaac acttcatcct gccagctctg atcaccctgt tttagatcca
     3421 cacgcagagg aatatttcgc gcatcacgat tcgggattgt tgaggcactg gcaacacggg
     3481 cgctctctgg caaggttgtg cgactggaga atggatcgtt ggtcagtagt tcccgatggt
     3541 tattatcccg ggaggcatcg ctaagcagtg tcgccagttt gtcgctcaga taagggcgca
     3601 aggcggtgat gtcgttgctg cggtgcaaaa tgcggtagtc ataaaattgc tgggccacgt
     3661 tatccgggcc tccttcaacg caaggaccac tgcgtgtgcc gttatcttta taagctggag
     3721 tgactgtggt gcaggcactg aggagcagtg cgcaggggat aagcattgtc aatttgctgt
     3781 agcgcataat gatttcctta taagcgatcg ctctgaaagc gttctacgat aataatgata
     3841 tcctttcaat aatagcgtat cagtctgata atgctttaga gatcgaaggc ttaacaaaca
     3901 aggagatcga tcatgcaatt ttctacaacc ccaactctgg aaggccagat catcgttgaa
     3961 tattgcggtg tggtgaccgg cgaagcgatt ttaggtgcca atattttccg tgatttcttt
     4021 gccggtatcc gcgatatcgt tggcggacgt tccggtgcgt atgaaaaaga actgcgtaaa
     4081 gcacgggaga tcgcctttga ggaattaggc tcccaggcgc gggcgctggg ggccgatgcc
     4141 gtcgtcggta ttgatatcga ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt
     4201 agcgtcagcg gtacggcggt gaaaacgcgt cgatgagaag agtcttctgg ctgatcgctg
     4261 ccgctctgtt actggcaggg tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc
     4321 agcttgatac ccgacgccag gcgcaggcgg cgtatccgcg cattaaagtg ctggtgatcc
     4381 actacaccgc agatgatttt gatagctcgc tggcgacact gaccgataag caggtcagct
     4441 cgcattatct ggtccctgcg gtaccaccgc gatacaacgg taaaccgcgc atctggcaac
     4501 tggtgccgga acaagaactg gcctggcatg cggggattag cgcctggcgc ggggcaacgc
     4561 gccttaacga cacctctatt ggcattgagc tggaaaaccg tggctggcaa aaatcggccg
     4621 gagtgaaata ttttgccccg tttgaaccgg cacagattca ggcgcttatt ccactggcga
     4681 aagatattat tgcccgttat cacatcaagc cggaaaacgt agtggcacat gcggatatcg
     4741 caccgcagcg caaagacgat ccggggccat tatttccctg gcagcaactg gcgcagcagg
     4801 ggattggtgc ctggccggat gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc
     4861 acacaccggt agataccgcg tcattgctgg agcttttggc gcgctacggt tatgacgtta
     4921 aacctgatat gacaccgcgc gagcagcggc gcgtgattat ggcattccag atgcatttcc
     4981 gcccgacgtt atataacggc gaagcggatg cagaaactca ggcgattgcc gaagcattgc
     5041 tggagaaata cgggcaggat tagcgcggca gttttccgtg gtcgcgtagc caggcggcag
     5101 ttttctcgat accttcatcc agggtgatga ccggctgata acctaactct tcctgcgcac
     5161 gcgtaatatc cagcgtaaag tcaaaattca acttggagac gccgtagtgg gtcagcggcg
     5221 gctcttttgc tgacttgcgg cctaaacgct ccatgctgcg ggcgatcata tccagcatcg
     5281 ggtaggggac ggaacgaata cgacagtcaa tattcaactc gtcgatcagc ttctgcacga
     5341 tgctgcgcag tgtgcgatgc tcgccgttgg tgatgttgta cacacgcccg gaaggtagct
     5401 tatcgcaggc ttcctggctt gccagccaca ttgcgtgcac ggcattttca tagtaggtca
     5461 tatcgaccag cgcactgccg ccatgcggta acagaatact gccgtagtgg tgcatcatat
     5521 gcgccagacg gggaataaag actttatcgt gcggtccgaa cagactttgt gggcgcagaa
     5581 tagtaaagcg cgtttgtgga ttcgcctgcg aaagcatatt gatcacttct tcgctggctg
     5641 ctttgctgcg ggcaaactcg ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc
     5701 gatggtggtg ataatcgaag tacagggagg gggaagagat atgaataaag ttacgcacac
     5761 cccaggcgac agtccattca cccaggcggc gagtggcgcg aacgttagcc agatcgaaag
     5821 cctgttgtgt cccccagggt gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg
     5881 cgagcatcac tttagcttgt gatgaaacca gctcggtcag atccgccgga acaaactctg
     5941 cgcccatttt ttccagcaat ttgcccattg cctcgttgcg accggtcgct cgcacgctga
     6001 tgcctttctg gcataaaaac tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa
     6061 ccagtacctt catatcaatc cactgttgtt gagaaaataa cgtgcgcatt cttccgtgat
     6121 ttcccccatg atgcaatggg aaacatgaaa gaataacgca ggttttgtcg attaatctgt
     6181 gctttgttct gccagtctgg cgatttgttt tgccattccg cgaaaaataa acagatgcgc
     6241 ggggatcatc aataaccagt aaaacagccc cggcataccg tgcggatgcc agaaagcgcg
     6301 gacatcgata gtactatagt cgcctttatc ttccaggcta aaacacagtc gtcccagccc
     6361 cggcgctttc atgccaaata acaacgtaag ttgtttttcc ggttcaacga caatcacttt
     6421 ccagctatcc accgcatcgc cagtctgtaa atattcgcgc tccgggcggc ctttcgccag
     6481 cttatgaccg atcgcgcggt ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa
     6541 ataacgctct ttaccgccga tttggttcac tacctgccat aaagcagcaa ggctggcgga
     6601 cgttttaacg gtaaaccccg cctgtttggc aaaataaccg tactctggtc gccagcgggc
     6661 aaaggcctga gcgtcgtagc cccagtcgct ggagttgacc agtttttcct cctctttcaa
     6721 cgtgctacgt accgcgtcat cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc
     6781 ggtatcatcc gccagcagat cgtgtttcag cccctgaatc aacgccctgg cggtggtggg
     6841 cggtacggaa gtaatcacat tgagaaacca caccgaaa
//
LOCUS       DAZFKM010000203         6863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFKM010000203 DAZFKM010000000
VERSION     DAZFKM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..667
                     /locus_tag="ACHKAY_004782"
     CDS             173..667
                     /locus_tag="ACHKAY_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703308.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
     gene            complement(698..1060)
                     /locus_tag="ACHKAY_004783"
     CDS             complement(698..1060)
                     /locus_tag="ACHKAY_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO5703309.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(1063..1335)
                     /locus_tag="ACHKAY_004784"
     CDS             complement(1063..1335)
                     /locus_tag="ACHKAY_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703310.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(1769..2065)
                     /locus_tag="ACHKAY_004785"
     CDS             complement(1769..2065)
                     /locus_tag="ACHKAY_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703311.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            complement(2106..2813)
                     /locus_tag="ACHKAY_004786"
     CDS             complement(2106..2813)
                     /locus_tag="ACHKAY_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5703312.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            2925..3149
                     /locus_tag="ACHKAY_004787"
     CDS             2925..3149
                     /locus_tag="ACHKAY_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703313.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3259..3537
                     /locus_tag="ACHKAY_004788"
     CDS             3259..3537
                     /locus_tag="ACHKAY_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO5703314.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            3573..3734
                     /locus_tag="ACHKAY_004789"
     CDS             3573..3734
                     /locus_tag="ACHKAY_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703315.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            3721..4542
                     /locus_tag="ACHKAY_004790"
     CDS             3721..4542
                     /locus_tag="ACHKAY_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5703316.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            4539..5915
                     /locus_tag="ACHKAY_004791"
     CDS             4539..5915
                     /locus_tag="ACHKAY_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO5703317.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            6002..6217
                     /locus_tag="ACHKAY_004792"
     CDS             6002..6217
                     /locus_tag="ACHKAY_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703318.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            6683..>6863
                     /locus_tag="ACHKAY_004793"
     CDS             6683..>6863
                     /locus_tag="ACHKAY_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703319.1"
                     /translation="MDESRKQFEEYVAKKLRLPFEMITEARNGDRYFAFSSMDIRHSL
                     NEWWTLWQASRAAIEL"
BASE COUNT         1861 a         1484 c         1648 g         1870 t
ORIGIN      
        1 ctaatttcat tttctggcga ccaacacaag tcacacccat ttcactgcgt ggcttgcggt
       61 agtaaagatt gtgcctgtct tttaaccaca tcaggctcgg tggttctcgt gtacccctac
      121 agcgagaaaa atagtaaaat cctcttaccc ctacagtaag agagtaattt atatggatgt
      181 gttatctggt ataactgccg cgaaacaggc ttatgatctg ctgaagacaa taaaagaaac
      241 cagagacgat gctgttatcg ctaaagctat tggtgatcta caccaaagaa taactgactt
      301 acagatgctt aatgcggagc tctctggcct ttatcaggcc gagaaggaga tcgcaatgaa
      361 gcttcgagat gaaaatagaa aaatcaagat gtttgttgtg caagctgaga attatgaact
      421 tcatacaact gaaggtggtt ctgtcgtgta tcgacctaag agtcattccg atccttctat
      481 tcagcagcat aacctttgtg cacattgctt cggcgaacat aaaatatcga tacttcaacc
      541 aagcacggtt actataaaat ctaatgggtt ctttgtgcat tcatgtcctc gctgtaagaa
      601 tgaataccgg atgtatagag cgccagaccc taagcctgta tatgtaccgc cgctgacaaa
      661 ttattaattt tattcctgaa atggcctctt caccccctta ctttgtcagg gggatatctc
      721 cttcagtttt gaccattcgc cttaatactt tccttaagtc gatgtaaagt tgaaggtctc
      781 catttgctgc ggcatcagcc attttttgcc tgacaagcag taatgtttca tacggctcaa
      841 taagaatatc gtcatgagta attaggtgaa gcgttgccgc atcaactatt cctagagctg
      901 cgccaagtat caaaaattcc ctgctatttt tgtcgcatga ggagataagc gtatttagcg
      961 catacctaat attctttata gctgttgtta atgctgcaat ttcttctatg gcgtcttctc
     1021 caatgagctt tttaagctca tatttttctt cctgacccat aattacctcg ccgtcagttg
     1081 ttttgatttc cggtagcctg ccgcgtaaat ggctacgttt ggcaggcaaa tacttccact
     1141 gcattcatct gccttcttgc agcgaaggct tccgagtgat gctgctttat ctgctctgac
     1201 gcaaccagag agctttagcg caatttttcg tgccaatcgc tgttcttgca ttgcctgttc
     1261 acgttgagcc tgtctgcgtg ctctgcggcg atttctggcg ttatcgtcag ccagatatgt
     1321 aatgactact gtcatgttga cctccgatga ttgactttgg cggtgacgcg ccgcgtgctt
     1381 atcttccggt tgccgtcttg cagctgcact tcacgtcacc ccaaagccaa ctactctttg
     1441 gttcccgcat ttcggcggga caatcccatc aatgttaaag agcctgccaa tctgttccgt
     1501 ttggcttcca gcgtcctgct gatggcttaa agataactta ggttataggt gtggtcaata
     1561 acctaattta tattttatgg taaataggtt ataagtgatg gataacaaag gtattttatt
     1621 tttgtaaatg ttgctgattg attggtgttt gagggcttgc gtgcggggtg aaggtgttac
     1681 ctttggcttg atgcttgtct atgatgagga tggttgattg ggtggtgaac ggcaggaaaa
     1741 gaaaacccgg cgctgaggcc gggtgttttt agtcttttct tttgcttagc atttcgtcga
     1801 tttctaagtc aatacgatat tgatctattg cttttctctc gtttggggtt ggtattttat
     1861 actgttcaat tagatctgtt gtgtatttta tttcatctaa tgtgattttt atatcagaga
     1921 gaatctcttt tatatcaatt atatgctctt cttcttttgc acgattagag ctatgttcaa
     1981 tcatttgaga tagcttttta ctaatgctta gcaaaacaaa aagaatgatg accaaaacaa
     2041 caacaaaaac tatcagaaat tccattatcc ctccgcactt ccgtaagtct tcttctgatt
     2101 atcggttacc aactatgaga cgaccagaat actctgccaa taatccttac ggtttcatga
     2161 aattcatctc tatccattac ttcatccggg tactcttcgc gatttattga tctgattatc
     2221 accgatgtag gggtggcgat taatgttttt actcgtaaca aatcagactg gcaaatagcg
     2281 taggttttac catctctgat tgtggtatct tgcgtgttaa caccaacaac atcgccatcg
     2341 tgaagcgttg gttccatgct ttgccctaca accctaacta gcttggctga tctttcagat
     2401 actcccatct ttttcagata gtgctttctg aaaaccaaag agaactccga tgattcctct
     2461 agctcgcagc taccgcttcc agctgaaagc gaaacgttaa gaagaggcaa cgcaacaaac
     2521 tcgtcatcgt ttcttttaat gtcttcccat accacagctt ttaaagatga atcacggaca
     2581 ttggatggtt cttcatgtgc accatccctc atttcaccaa taccagaact aagccattca
     2641 gggcgcactt ttaaagcatt ggctaattca accatcttgc gagatccgtt tgttttaccg
     2701 gacgacatct tctgtatggc tggctgagat attccaacca tgtcagcaag ctgtgattgt
     2761 gatacccctg ctgagctcat ggctgcattt agtctttctg cgaatgtttt catacccaca
     2821 aatctataac tacggttatc caaagtaaaa taacaaaggt tattgctatt ttttataact
     2881 tgagttatct ttggttataa gtaatgacca caagaggtat gctcatgaat ttagtaattc
     2941 aacgagcctt gaaaattgtc ggtagccaaa agcgccttgc cgacaagtgt ggtgtaacgc
     3001 agccagcagt acacaaatgg ctgaaaggcg ggttggtctc tccagagaaa gttaccgcca
     3061 tcgttaacgc cactggaggg cagatcaagg cttacgaaat tcgccccgat ttgccacacc
     3121 tgtttccaaa accgaatcag gcagcataag taacaccgct ctttaacagt catggtcctc
     3181 attcccgccg aaatgcggga atacaacgcg cataagttga tgcgcataac ttcttatttg
     3241 ttaaggaaat acttacatat ggaacttaca agtactcgca agaaagcgaa tgcaattaca
     3301 agcaacatcc tgaatcgaat tgctgtacgt ggtcagcgaa aggttgctga tgcattaggg
     3361 atcaatgaat cgcaaatttc gcgatggaaa gacagcttta tcccaaagat ggccatgctt
     3421 ctggctgtgc tggagtgggg tgttgaagac gaggaattag cggagctggc aaagaaagta
     3481 gccatggtgc tgacaaaaga aaagcctcaa gactgcggca acagttttga ggcctgattg
     3541 tagaaagact gtatcaatcc acaggagtaa ttatgacaaa acgtcgtaag aaataccagg
     3601 aaaaagaaga gattcgacac cctgattcac ctgagggatt agtggtagcc gcagcaaata
     3661 acagggcgtt cgcagagcgc cttgttggtg tttacagact agccaaagca ggagtgaaac
     3721 atgggcgtcg ttaagttagc tgattacagg cctcaactgg aggtcgtgga gcatcgcgtg
     3781 gcagataccg aagatggttt catgcgcgtt gctaacgaga ttatcgacag tctgctgatg
     3841 gctgatttaa ccgtccggca gatgaaggtg atgctcgcta tcatgcgcaa gacatacgga
     3901 ttcaataagc cgatggatcg actcacaaac acgcagatag cagccatgac aggtattcat
     3961 cacactcatg tttgcgctgc caagcgccag cttattgagc gtaaattcct cattgctgat
     4021 ggcgtgaaaa tcggagtgaa caaggtggtt tctcagtgga ttagccagga cagcttaaca
     4081 ttagctaaaa cagctaataa aacattagcc gagtcggcta atgggtataa gccaagtcag
     4141 ctaaacacaa aagacaatat acaaaagaca ataaatacaa ataccccctt accccctaac
     4201 gggggcggcg atgggcaggt taaacctgaa cgtcgcaagg cagaacgaat cgactacgaa
     4261 tccttcctga acgcctacaa caccgaagtc ggtgacagac ttccacacgc tgttgcggtc
     4321 aacgagaaac gaaaacgccg cctgaagaaa atcatcccgc aactgaaaac gccaaacgtg
     4381 gacggtttca gagcgtatgt cagggcgttt gtgcatcagg ccaagccgtt ttacttcgga
     4441 gacaacgaca cgggctggac ggcagatttt gattacctgc tgagggaaga ctcgttaacg
     4501 ggagttcggg aagggaagtt tgcagacagg gggattgcat gagacaggat atcgaagcga
     4561 gcgttatcgg tggcttgctg attggtggat taacaccaac cgccagcgac gttctggcaa
     4621 cgctggagcc ggaagcgttt tcaattccgc tctaccggaa agccttcgaa gttatccgta
     4681 agcaggcgag aaacagaaac ctaatcgacg cgctgatggt tgccgaggcg tgcggagagg
     4741 agcatttcac gtcaatcctg atgaccagca aaaactgccc gagtgccgca aacctgaagg
     4801 gatatgccgg aatggtcgcg gataactatc accgccgtct ggtgctggaa atcatggacg
     4861 aaatgcgtga accaattcag agcggaacca tcgatacatc gagtcaggcg atggacgagc
     4921 ttgtaaagcg tctctcagcc atcagaaagc cccgtgacga ggttaaacct gtacggttag
     4981 gggaaatcat cactgactac actgacacgc ttgacaggcg tctgaggaac ggagaagagt
     5041 cagataccct gaagaccgga atcgaagaac ttgacgccat caccggaggg atgaacgcgg
     5101 aagacctggt gattatcgcc gctcgtcctg gtatggggaa aaccgaactg gcgctgaaga
     5161 ttgccgaagg tgttgcaagc cgcgttattc ctggttctga cgtccggcgc ggagtgttga
     5221 ttttctcgat ggaaatgagc gcattgcaga ttgcagagcg gagcattgcc aacgccggga
     5281 ggatgtcggt tagcgtactg cgaaatcctg catcgatgga tgacgaaggc tgggcgcgtg
     5341 ttgctaacgg catgagtcag cttgcagatt tggatgtatg ggtagtcgat gcctcgcggt
     5401 tatcggtaga agaaattcgc tcaatcgcag agcggaacaa acaggaaaat ccaaacctgt
     5461 cactcatcat ggcggattat cttggcctga ttgagaagcc gaaagcagac cgcaacgacc
     5521 tcgcaattgc tcacatctca ggaagcctga aggcgatggc gaaagacctg aaaacgcctg
     5581 ttatctccct aagtcagctt tcgcgcgatg ttgagaagcg accaaacaaa cgcccgacaa
     5641 acgcagattt gcgtgattca ggaagcattg agcaggacgc agactcaatc atcatgctct
     5701 atcgggaagc ggtatatgac gagaacagta gcgccgcacc atttgctgaa atcattgtga
     5761 cgaaaaaccg gtttggccca cttggtacgg tttaccagcg gttctgtaac ggacactttg
     5821 ttgcatgtga ccaggatgaa gccagacaga tttgcacagc atcaaatgca cctgcttcgc
     5881 gtggcagacg atatgcacaa ggggctgacg tatgaccatc tacatcactg agctaataac
     5941 aggcctgctg gtaatcgcag gcctttttat ttgggggaga gggaattgga ggctttaaga
     6001 aatgagtacg atagctgagc ttgtcagggc taattttcgt gaagagttgg tgcgttggta
     6061 tcggtatcgt tcatcgtcca gtttgccgat tgatgagttg tatgagcatt cacctgctgc
     6121 acgacgctat ccgcgtgacc gtgtccttcg acggttgttc aaactcaaca atgagtttca
     6181 gcgcaacaga ataatccgga gtctggattt aaagtgaagg agtgagcatg agtcgaagaa
     6241 gtagcttttt ggggtttgta atattcctgt cctgcactgg ttacatcgta atctggtcaa
     6301 tttcgaacat tgaccgtggc ggggaatatc tcattgtaat gttctttcct ttgtttcttg
     6361 ggtggtacgc cgcaaggttg ctggaagaat ggggttacag gcataaaaaa taaaggagtg
     6421 ttcagtgaag caaacccttt tttattggat taagagcgag gggtaagtac cgatggtaaa
     6481 tgcatttatt tgtagtttat ttcttgtcgc gatttttcat ggattccttc tgatgatgag
     6541 ttttgttctc tggaataatg gatatcgcat attgggagta ggttttgttt tacggttttc
     6601 agttgtctgc gcgttgctac cgataattat ggcgactatc aaatattatt ggtaacccaa
     6661 aaaatcatcg atggagagtg atatggacga atcaagaaag cagtttgagg aatacgttgc
     6721 caaaaaattg agattaccat tcgagatgat aaccgaggca agaaatggtg ataggtactt
     6781 cgcattttca agcatggata ttcgtcactc cttaaatgag tggtggactt tatggcaggc
     6841 atcgcgagca gctattgaac tgg
//
LOCUS       DAZFKM010000204         6824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFKM010000204 DAZFKM010000000
VERSION     DAZFKM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            591..1307
                     /gene="nanC"
                     /locus_tag="ACHKAY_004794"
     CDS             591..1307
                     /gene="nanC"
                     /locus_tag="ACHKAY_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO5703320.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1327..2433
                     /gene="nanM"
                     /locus_tag="ACHKAY_004795"
     CDS             1327..2433
                     /gene="nanM"
                     /locus_tag="ACHKAY_004795"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO5703321.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2498..3478
                     /gene="nanS"
                     /locus_tag="ACHKAY_004796"
     CDS             2498..3478
                     /gene="nanS"
                     /locus_tag="ACHKAY_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO5703322.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(3486..4136)
                     /locus_tag="ACHKAY_004797"
     CDS             complement(3486..4136)
                     /locus_tag="ACHKAY_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5703323.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            4273..4416
                     /locus_tag="ACHKAY_004798"
                     /pseudo
     CDS             4273..4416
                     /locus_tag="ACHKAY_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(5080..5208)
                     /locus_tag="ACHKAY_004799"
                     /pseudo
     CDS             complement(5080..5208)
                     /locus_tag="ACHKAY_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5184..5333)
                     /locus_tag="ACHKAY_004800"
                     /pseudo
     CDS             complement(5184..5333)
                     /locus_tag="ACHKAY_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(5418..5615)
                     /locus_tag="ACHKAY_004801"
     CDS             complement(5418..5615)
                     /locus_tag="ACHKAY_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5703324.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(5627..6115)
                     /locus_tag="ACHKAY_004802"
     CDS             complement(5627..6115)
                     /locus_tag="ACHKAY_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5703325.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(6112..6486)
                     /locus_tag="ACHKAY_004803"
     CDS             complement(6112..6486)
                     /locus_tag="ACHKAY_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5703326.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(6576..>6824)
                     /locus_tag="ACHKAY_004804"
     CDS             complement(6576..>6824)
                     /locus_tag="ACHKAY_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5703327.1"
                     /translation="LHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNP
                     RHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK"
BASE COUNT         1925 a         1483 c         1627 g         1789 t
ORIGIN      
        1 ataattaaat aacaaccctt ggtttgtgaa ggcctccgca agtgttcgtt tggcttcaca
       61 tggcatcttc ttcttagaaa aatatcgaca tattttgtga cacgaattgc aaatctggtt
      121 ttgttgtatg gattgcgtga tttttgatct ggtataacag gtataaaggt gcaccaagat
      181 agtcaatgag acagggcatc tcgcaatcta tggcaaacat cacttcagtt ctttcacatc
      241 gggtgatgaa aatgcacttc agtctgaaag gaatatgaaa atgagatcaa cagacactct
      301 attttatgac tctgggtaaa atggattgag taagtgatat agcttacgaa cattcaaatc
      361 aattaaacat cagaagagat tttatactca ggtatttaat ctggctctct gtttatttaa
      421 ataatgtgaa aagagatttt tcacaggaga ccttatacaa aaaatataaa atacagctac
      481 cggttgccaa agacactata agcctggcaa aaaaatatta cacaacataa atgctaattg
      541 tttatgcggg ctttgtattg ctttctgttt cctacaaatg agtgaaattt atgaaaaagg
      601 ctaaaatact ttctggcgta ttattactgt gcttttcgtc accattaatt tctcaagctg
      661 cgacactgga cgtacgtggt ggatatcgta gtggaagcca cgcctatgag actcgactca
      721 aagtcagtga gggatggcaa aatggatggt gggcaagtat ggaaagtaat acctggaata
      781 ccattcatga taataaaaag gaaaatgccg cactcaatga tgttcaggtt gatgttaatt
      841 acgcgattaa acttgatgat caatggacgg tgcgcccggg aatgttaacg cattttagca
      901 gcaacggcac acgctacgga ccctacgtaa aactgtcctg ggacgcgaca aaagatctta
      961 attttggcat tcgctatcgt tacgactgga aagcttaccg acaacaagac ttatccggtg
     1021 atatgtctcg tgataacgtt catcgttggg atggatatgt cacttaccat attaatagtg
     1081 atttcacctt cgcatggcaa acgacgctat acagcaaaca gaacgattat cgctatgcaa
     1141 accataagaa atgggcgacg gaaaatgcat ttgttctaca ataccatatg acgccggata
     1201 ttacgccata catagaatat gactaccttg accgtcaggg tgtttacaac ggcagagata
     1261 atttatcgga aaacagttat cgcattggtg tgtcatttaa actgtagtag acaggagaca
     1321 gtcacaatga ataaaacaat aacggcgctt gctatcatga tggcttcatt tgccgcaaac
     1381 gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg gtaccggagc aattgataac
     1441 gacactgtct acattggttt aggtagcgca ggtacggcat ggtacaagct ggatacacag
     1501 gccaaagata aaaaatggaa agcgttagct gcattccctg gtggaccaag agatcaagca
     1561 acctcggcat ttattgatgg caatctgtat gtgtttggcg gcattggcaa aaacagcgag
     1621 ggcttgactc aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa tagttgggtt
     1681 aaattgatgt cgcacgcacc gatgggcatg gcgggtcatg taacttttgt acacaacggc
     1741 aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt tgaagatctc
     1801 aacgaggctg gaaaagattc aaccgctata gataaaatca acgctcacta ttttgacaaa
     1861 aaagcagaag attatttttt caataagttt ctgttgtctt ttgatccctc aacacagcaa
     1921 tggagttacg ctggcgaatc gccctggtac ggaacggctg gtgcggcggt tgtgaataaa
     1981 ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac ggatgccgta
     2041 tttgaacttg atttcaccgg taataattta aaatggaata agcttgctcc cgtctcatca
     2101 ccagatggcg tagctggcgg ttttgcgggg ataagcaatg attctcttat atttgccgga
     2161 ggggccggat tcaaaggttc acgagaaaat taccagaacg gtaagaacta tgcgcatgaa
     2221 ggcctgaaaa aatcatatag cactgatatt catctttggc ataacgggaa atgggataaa
     2281 tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat tgccctggaa taatagtcta
     2341 ttgattattg gcggtgaaac tgcaggcggc aaagcggtga cggattcagt tttgatctct
     2401 gtgaaggata ataaagttac agtacaaaac taacgcttca gggccccggt aaaggggccc
     2461 tgctatcaat ttgtcatttc aattacgcga aatttatatg aacgcaataa tatcgcccga
     2521 ttattactat attcttacca ttgctggtca gtctaatgcc atggcgtatg gcgaaggact
     2581 gccattaccg gacagggaag atgcgcctca tcccagaatc aaacaattag cgagatttgc
     2641 gcatacgcat cccggaggcc cgccatgtca ctttaacgac atgattccac tgactcactg
     2701 cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacga atcatcaaac
     2761 acagtacggc accgttggcc aggcactgca tatagcacgg aaattactgc cctttattcc
     2821 tgataatgca ggggttctca tcgttccgtg ttgccgtggc ggatcggctt ttaccgcggg
     2881 cagcgaaggg acatattcag aacggcacgg agccagccat gatgcttgtc gttggggaac
     2941 ggatactccg ctataccagg atttagtcag cagaacgcga gccgcactgg caaaaaatcc
     3001 gcataacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa tgaccagtga
     3061 ctacgcgtca caccctcaac actttaatca tatggttgaa gcctttcgta gggatctaaa
     3121 acaataccat tctcagctta ataatattac tgacgcaccg tggttttgcg gcgataccac
     3181 ctggtactgg aaagaaaatt tccctcatgc gtatgaagtt atttatggca attatcaaaa
     3241 taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa gaggactgac
     3301 gaacgcgcct gatgaagacc cggacgattt aagcacggga tattacggtt cagcgtaccg
     3361 gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcacgg cagcccgtcg
     3421 ggggattatt tctgacaagt ttgtagaagc aattttgcag ttttggcgcg aaaggtgagc
     3481 gttcattaat ttgatttatt cgcatccatc agcgctttta actgctccgg cgtaaacggt
     3541 ggtttgcgtc ggtgcaacat cgcatggcaa ttagggtaca caggcagcaa atcattaact
     3601 ggatccagct ggtaatcctc tttaatggct gacagcggca ccaggtgatg cacatggata
     3661 aagccttcag cgatatcacc atagatcttc accagatcaa caccgcatac gttgcactgg
     3721 catccatgat gttcgaccgc tttatctcgg gcctttttat cgcgttcata gcggtttact
     3781 gtcaccttta tcgccgcacc ttcaaggtat gtctcctgcg acggcaactc atccgggaac
     3841 agaacgtcgg ctatgggagc cgcagcggct ttatccagat gaatatgaaa tccctgctct
     3901 atgagtagct tgacacattt agattttatt ccgccggtga aatccgcagg ggtaaattgc
     3961 gtccccgtca tcaatgtcgc tgcgatgcca gcaatcgcct tcgaggggta acgtcggcca
     4021 tgatattcaa tctcatacaa ccgcgcaggc tggaactgat gagtttctcc cgcatcccat
     4081 aaatggatag cttcgatgat ataccgccgc ggaagatgat cagggagttt attcatggag
     4141 aatgtcctta tacgttctca gaacaggcag caaaaaaaca aatgagatca aggtaatata
     4201 tcttcacttg caggcttgaa cagcagaaaa tttgcgagca tgatcgcaat tatttcactg
     4261 ccagggatat caatagcttc cagtaaatcg ctacagcagg caattgccaa tataaaaatc
     4321 tggcacaagg gtgaacagcg cgcgccgcat aagccattgt tattgctata cgtattagcg
     4381 ggatacctga atggacatcc gcgccttttc gattattgta gtggaacagt ctacgaggat
     4441 gcagttcgaa gtgagtaagt aaaggttgcg ggaaacgatc aatgccgcca tacaatgatt
     4501 ttttaggatc aacaatgagt aataaaaact acgaaagtca ccgcaaagcg attgttagta
     4561 aaggtatacc acccgctcta ttaaataggc tcaccaattc ggatgttcag gtgatcaata
     4621 ccttcttaac acgagtgagc aaactggaac tatctcaaca agagaaagac tggatcataa
     4681 aaatcatctc tatggtttag acaatttcag ccttgtagcc tttcaagggt tttgtgtggg
     4741 tacaaacatt atcgaatcgg ggtatagccg cagctgtagc tgtatccaca tcagtaagtt
     4801 gatgtaggtt gacttaggtt gagcgaaaaa tagcgaaaag ccttgtgtgg cgcggatttc
     4861 agtcataaaa aaacgtccgt tgatgtctat tgatgttccg atggtgcgaa ggtgcgaagg
     4921 tgcgaaggcc ggactcaaac atgtgactta acctcatgat ttaaaattgt taataataaa
     4981 caatatcatc ttataccaac acagatacca acacgaaaaa tcactggttt tcggcatcga
     5041 accaggacaa tcagctcctg attgagatac cgagctctgt tactcgtcat cttcctcgac
     5101 aggcttcatt gcatcaagta gtactcgcac catcgcgata gaaccaatcc ggataaaagc
     5161 atcttctcca tctatatctg ttaactgata tcgctcactt gatgccggat aattgtgtcg
     5221 atttgcagca gtgaactctt tcgtacagcc agaatgcggc ggatgctggt tgagcagata
     5281 acgtccgtat tctcgttgac cacttccgga cggtaggctg tgatatcaga cataatcgat
     5341 attccttttt gcagataaat gaaaatgcca gccctgttca ggctggcgtc agtggaatga
     5401 agtctggtga gtaagactca ccggttctgg tgctgaagct gcagttggcg cagggtacgg
     5461 atatcctctc tggcctgttc gatacagggc aacagtgctg ctgaatccgt tttatcctca
     5521 ttgttgtcaa agataattcc cgattcgcag tcgatattgt cctgcagcca cgcaatcaga
     5581 atatccagtg cggtttccgt ggttaatgat ttcatgttgt tcctggttac cagttgaaag
     5641 tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg acgccataac
     5701 gtcgggtaag tgtgataacc agacgacgga acgtttttga cagccccagg cgtttaagcc
     5761 gcagaaccgg atatgaccac gcatcggtac gtaacagata tccgctacct gtgaaatgaa
     5821 tccattctga ttcaccaacg tcactggtct gatgcgacag cgagtacagc caggcgttat
     5881 ccttttccgt gatatgtgcg gtactgcagc gtatgccggc aagggttgca aacggtggta
     5941 gtgtgcaggt tgactgttgg tcggattcct ccaccacgcg gagtgaataa ccgttttcag
     6001 cgaccttgtt aatcagttca gtgagattaa caccatcgac gtcaacgaca attcgcccca
     6061 tgttcagtgc ctgcacatta attctgtcgg cttccagcat cagggaaagt ttcatcgttt
     6121 ctccggatgc ttacccaggg taatgttatt taccgttctg taattgtcgc gggtcatcag
     6181 gccggtcgcc ctgcgagccc ggaggatatc aatactgttt attaactgag agcggggaca
     6241 agtgctgaat cccggctggt cggtacgcac cagcgcgtat ttttccacga gaaaattcac
     6301 cgcatcacac agtgaaatgc cagcttcaat atgctgctca atcacacgtt catcggcgaa
     6361 cggtgtgtca ttcagtgtaa ggccgtaatg ctggcccagt agtcgggaga gcagagtctg
     6421 ccagatggtg acgggagacg ggcagctcga tgcctcccgt acgtgtgtat cgggtaatgt
     6481 tttcatactg aaggttttcc tgatatgcag agatgaaaaa tggaaaagtg gcgtggtgaa
     6541 agcaccaggc cggagcagaa ggttattccg gtggtttact ttttcgtttc gggtgtcgga
     6601 taaacagcca gataaacgta accaccgcta ccgagagtgt cagctttgca ggtcagacct
     6661 tttgcataca gcgtgacggt atgctgatgg cggggattca gttcaccgct ggtgagcatg
     6721 agttccagtt gtttcatcag cagcggaaag gcctggtcca ggtggtacgc atctgcatcg
     6781 ctgaacaggc ctctgatacc ggcgcggtcg gcaaggtaat gcag
//
LOCUS       DAZFKM010000205         6811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFKM010000205 DAZFKM010000000
VERSION     DAZFKM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..1311
                     /gene="acrE"
                     /locus_tag="ACHKAY_004805"
     CDS             154..1311
                     /gene="acrE"
                     /locus_tag="ACHKAY_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO5703328.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1323..4427
                     /gene="acrF"
                     /locus_tag="ACHKAY_004806"
     CDS             1323..4427
                     /gene="acrF"
                     /locus_tag="ACHKAY_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO5703329.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4680..4901
                     /locus_tag="ACHKAY_004807"
     CDS             4680..4901
                     /locus_tag="ACHKAY_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703330.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5332..6357
                     /locus_tag="ACHKAY_004808"
     CDS             5332..6357
                     /locus_tag="ACHKAY_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5703331.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6425..>6811
                     /locus_tag="ACHKAY_004809"
     CDS             6425..>6811
                     /locus_tag="ACHKAY_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5703332.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIE"
BASE COUNT         1650 a         1627 c         1751 g         1783 t
ORIGIN      
        1 gtgtttatgt gccttgagac gccagtattc ataactattc cttacatcga cgaatgataa
       61 tttgtaggat agcaaactgt atttttcttt ctgcgagtta acgcgttacc tttctgggta
      121 aataacgcgc ttttggtttt ttgaggaata gtaatgacga aacatgccag gtttttcctc
      181 ctgccctcct ttattctgat ctccgcggct ttaatcgccg gttgtaacga taagggagaa
      241 gagaaagctc acgtcggtga accgcaggtt accgttcata ttgtgaaaac ggccccgtta
      301 gaagttaaga ctgaattacc aggccgcacc aatgcttacc gtatagccga agttcgccca
      361 caggttagcg ggatcgtact gaatcgtaat ttcactgaag gcagcgatgt acaagcaggc
      421 cagtccctgt accagatcga tcccgcgacc tatcaggcaa attatgacag cgcgaaaggc
      481 gaactggcga aaagtgaagc cgccgccgcc atcgcgcatt tgacggtaaa acgttacgtt
      541 ccgctcgtgg gtacgaaata catcagccag caggagtacg accaggccat tgctgatgct
      601 cgccaggccg atgccgccgt gattgccgca aaagccacag tcgaaagcgc tcgcatcaat
      661 ctcgcttata ccaaagtgac tgcgccaatt agcggacgta tcggcaaatc gaccgtgacc
      721 gaaggcgctc ttgtcactaa tgggcaaacg actgaactgg cgactgtcca gcagctcgat
      781 cctatctacg ttgatgtgac ccaatccagc aacgatttta tgaggctgaa gcaatccgta
      841 gagcaaggaa atttgcataa ggaaaacgcc accagcaacg tagagttggt catggaaaac
      901 ggtcaaacct atcccctgaa aggtacgctg caattctctg atgtgaccgt tgatgaaagc
      961 accggctcca taaccctacg tgctgtcttc cctaacccgc aacatacgct tttgccgggt
     1021 atgtttgtgc gtgcacggat tgatgaaggc gtccaacctg acgccattct tatcccgcaa
     1081 caaggcgtta gccgcacacc gcgtggtgat gcaaccgtgc tgattgttaa cgataaaagt
     1141 caggtggaag cgcgccctgt cgttgccagt caggcgattg gcgataaatg gttgattagc
     1201 gaaggtctga aatctggcga tcaagtcatt gtcagcggcc tgcaaaaagc gcgtccggga
     1261 gagcaggtta aagccactac cgataccccc gcagatactg catcgaagta aggtaatctg
     1321 acatggcaaa cttttttatt cgacgaccga tatttgcatg ggtgctggcc attattctga
     1381 tgatggcggg cgcactggcg atcctacaat tgcccgtcgc tcagtatcca accattgcac
     1441 cgcctgcggt ttctgtttcg gcaaactatc caggtgctga tgcgcagacc gtgcaggata
     1501 cggtgacgca ggttatcgaa cagaatatga atggtatcga taacctgatg tatatgtcct
     1561 ccaccagcga ttccgccggt agcgtgacaa ttacccttac cttccagtcc gggaccgatc
     1621 ctgatatcgc gcaagtgcag gtgcagaaca aactccagct cgccacgccg ttgctgccgc
     1681 aggaggttca gcagcagggg atcagtgttg aaaagtccag tagcagctat ttgatggtgg
     1741 cgggctttgt ctctgataac ccagacacca cacaggacga tatctcggac tatgtggcct
     1801 ctaacgttaa agatacgctt agccgtctga atggcgtcgg tgacgtacag cttttcggcg
     1861 cacagtatgc gatgcgtatc tggctggacg ccgatctgct aaacaaatat aaactgacac
     1921 cggttgatgt gattaaccag ttgaaggtac agaacgatca gatcgctgcc ggacagttgg
     1981 gcggaacgcc agcgttacca gggcaacagt tgaatgcctc gattattgct caaacgcggt
     2041 taaaaaatcc ggaagaattc ggcaaagtga ccctgcgcgt aaacagtgac ggctcggtgg
     2101 tacgcctgaa agatgtcgca cgggttgaac ttggcggtga aaactataac gttatcgctc
     2161 gtatcaacgg aaaaccggcg gcgggcctgg ggattaagct ggcaaccggc gcgaatgctc
     2221 tcgataccgc gaaagccatt aaggcaaaac tggcggaatt acagccattc ttcccgcagg
     2281 gaatgaaggt tctctaccct tatgacacca cgccattcgt ccagctttct attcacgaag
     2341 tggtaaaaac gctgttcgaa gccattatgc tggtgttcct ggtgatgtat ctgttcttgc
     2401 agaatatgcg agcaacgctg atccccacca ttgcggtacc cgtggtgttg ttagggacgt
     2461 ttgccatcct cgccgctttt ggttactcca tcaacacact aacgatgttc gggatggtgc
     2521 ttgccatcgg gctgctcgtc gatgatgcga tagtggtggt ggagaacgtc gagcgcgtga
     2581 tgatggagga taagctcccg ccaaaagaag cgacggaaaa atcaatgtcg caaatccagg
     2641 gcgcactggt gggtatcgcg atggtgctgt cagcggtatt tattccgatg gcattcttcg
     2701 gcggttctac tggggcaatt tatcgccagt tctctatcac catcgtttcg gcaatggcgc
     2761 tttctgttct ggtggcattg attcttaccc ctgcgttatg tgcaacgctg cttaaacccg
     2821 tctctgctga gcatcacgaa aataagggcg gtttcttcgg ctggtttaat actaccttcg
     2881 atcatagcgt taaccactac accaacagcg tcggcaaaat cctcggttcc acaggacgat
     2941 atttactgat ctatgcgctg attgttgcag gaatggtggt gttgttttta cgtcttccgt
     3001 cttccttctt acctgaagag gatcagggtg tctttctgac catgattcag ttacccgctg
     3061 gcgcgacgca agagcggacg caaaaagtgt tggatcaagt gacggattac tatctgaaga
     3121 acgagaaagc gaacgttgaa agtgtcttta cggttaacgg ctttagcttc agcggccagg
     3181 cacaaaacgc cggtatggcc ttcgtcagtc tgaaaccgtg ggaagagcgt aatggtgatg
     3241 aaaacagtgc ggaagcggta atccatcgtg ccaaaatgga atttggcaag atccgcgacg
     3301 gttttgtcat tccattcaat atgccagcca ttgttgaact gggcacggca acgggtttcg
     3361 actttgagtt aattgatcag gctgggctgg gtcacgatgc cctaacccag gcccgtaacc
     3421 agttgcttgg tatggcggcg caacatcctg ccagcttagt cagcgtgcgt cctaatggcc
     3481 tggaagacac cgcgcagttt aaactggaag ttgaccagga aaaggcgcag gcattaggtg
     3541 tttcactttc tgacatcaat cagaccattt caacggcgtt gggtgggact tacgttaacg
     3601 acttcatcga ccgtggtcgc gtgaaaaagg tgtatgttca ggcggatgcc aaattccgta
     3661 tgctgccaga agatgtcgat aaactttatg tccgcagcgc caacggcgaa atggtgccat
     3721 tctcggcctt taccacttca cattgggtgt atggctctcc gcgactggaa cgctacaacg
     3781 gtctgccgtc aatggagatt cagggggaag ccgcgccagg aaccagttcc ggcgatgcca
     3841 tggcgttgat ggaaaacctt gcgtcaaaat tacctgctgg cattggttat gactggacgg
     3901 gtatgtcgta tcaggaacgc ttatcgggaa accaggctcc cgctctggta gcaatttcct
     3961 ttgtggttgt tttcctgtgc cttgctgcac tctatgaaag ctggtcaatt cctgtctcgg
     4021 ttatgttggt cgtgccgtta gggattgtcg gcgtgctgct ggcggcgaca ctctttaatc
     4081 aaaaaaatga cgtctacttt atggtgggct tgctaacgac aattggcttg tcggccaaaa
     4141 acgctatttt gattgttgag ttcgctaaag atctcatgga gaaagagggt aaaggtgttg
     4201 ttgaagcgac actaatggca gtacgtatgc gtctgcgtcc tatcctgatg acctctctcg
     4261 cctttattct cggcgtatta ccgctagcta tcagtaacgg tgccggcagt ggcgcgcaga
     4321 acgcagtggg tatcggggta atgggcggaa tggtctctgc aacgttgctg gcaatcttct
     4381 tcgtaccggt gttctttgtg gtgatccgcc gttgctttaa aggataaatc agaaacataa
     4441 aggcactttc gggtgccttt attattttca gtgaaaccca taaaaattaa taagatattc
     4501 ttccactcac tctttaaaaa ctttctatag ttcccgctcc cttcactatt tttacaattc
     4561 acataatttg tcattattca ttccgcaata tttataacct ccggtgataa aatggcattc
     4621 agctcgttaa taagagagtt aacttattaa gcgttagcgt ttattactga ggtaacacca
     4681 tgaaaagatt aattcctgtg gcattgctca ccgcattgct ggcgggctgt gctcacgatt
     4741 cgccctgcgt tccggtttat gacgatcagg gtcgtctggt tcacaccaat acctgtatga
     4801 aaggcacgac tcaggataac tgggaaacgg ctggggctat tgccggcggg gcagcggctg
     4861 ttgctggcct gacgatgggg atcatcgctc tgtcgaaata actgaacagg gcgcggagtt
     4921 tcgcgctctg tttaacgata cagtatcttt acataagata aaatattgct atattctgct
     4981 ggctaattct ctgctgattt agcatgttat atcgcgttat ttcgctgctt tcgaaataac
     5041 gcatcaattt aactttcaca cttcccacca gaaatatact cacgctaaca atcattattc
     5101 tgcttagtaa aacacgcgta aatattttgc gccaaaatgt ggcgcatgtt tcattttcgc
     5161 accattgcgg ggcgctgttt ttattatttc ctgtctacac tcagcttatt gcgacgcgaa
     5221 atcgggcgca aaagctggca ctacttttgc ttataagagg atggccacag acaggtaaaa
     5281 gacgtttccc aaacgtccta taacgataat tttcgcctca caggaagcat tatgaaaaag
     5341 atgatgatag ccacactggc tgccgccagc gtgctgcttg ccgttgcaaa tcaggcgcat
     5401 gctggagcga cgcttgatgc cgttcagaaa aaaggttttg tgcaatgcgg gatcagtgat
     5461 ggattacctg ggttctctta tgccgatgca gacggtaagt tttcaggtat tgatgttgat
     5521 gtttgtcgtg gtgttgccgc tgctgtattt ggtgacgaca cgaaagtgaa atatacccca
     5581 ctcacagcaa aagaacgctt cacggcttta cagtcagggg aagtggattt gctctcccgt
     5641 aatacgacct ggacttcatc tcgcgacgcc gggatgggaa tggcatttac tggcgtcact
     5701 tattacgacg gcattggctt cctgacgcac gataaagcgg ggctaaaaag cgcgaaagaa
     5761 ctggatggcg ctaccgtctg tattcaggcg ggtactgata ccgaactcaa cgttgccgac
     5821 tatttcaagg caaacaatat gaagtacaca ccggtgactt tcgatcgctc tgacgaatca
     5881 gcgaaggcac tggaatctgg tcgctgcgat acgctggcct cggatcagtc gcaactgtat
     5941 gccctgcgca tcaaattaag caacccagct gaatggattg tcttaccgga ggttatctct
     6001 aaagaaccgc ttgggccggt agttcgtcgt ggcgatgatg aatggttctc gattgtacgc
     6061 tggacgcttt tcgccatgct gaatgctgaa gagatgggca tcaattccca gaacgtcgat
     6121 gaaaaagcgg ctaatccagc aacgcctgat atggcacatc tgctgggtaa agaaggcgat
     6181 tacggcaagg atctgaagtt ggataataaa tgggcctata acatcatcaa acaggtgggt
     6241 aactactcgg aaattttcga gcgtaatgta ggttcagaaa gcccgctgaa aattaaacgt
     6301 gggcaaaata atctctggaa taacggcggt attcagtacg cgccgcccgt gcgttaagtc
     6361 ggtgtgaggt aacgagcgtc gctacggcgg cgctctcttc agcgttatgc tttccgaggt
     6421 ttctatgtct catcgccgct caaccgttaa aggctcactc tcctttgcca accctacggt
     6481 tcgtgcctgg ttattccaga tccttgccgt tgttgctgtt gtcggcattg ttggttggtt
     6541 atttcacaac actgtaacga atctcagtaa tcgtggcatt acttcaggtt ttgcctttct
     6601 ggatcgcggc gctggcttcg gtattgtcca gcatttgatc gattaccagc agggcgacac
     6661 ctacggacgc gtttttattg tcggcttact caatacgcta ctggtttctg cattgtgtat
     6721 tgtgttcgct tctgttttgg gcttctttat cggtctggcg agactttcgg ataactggct
     6781 gctacgaaag ctttccacaa tttatattga g
//
LOCUS       DAZFKM010000206         6750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFKM010000206 DAZFKM010000000
VERSION     DAZFKM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKAY_004810"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHKAY_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO5703333.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKAY_004811"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHKAY_004811"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO5703334.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKAY_004812"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHKAY_004812"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO5703335.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKAY_004813"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHKAY_004813"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO5703336.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKAY_004814"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHKAY_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO5703337.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1474 a         1832 c         1831 g         1613 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata
//
LOCUS       DAZFKM010000207         6694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFKM010000207 DAZFKM010000000
VERSION     DAZFKM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1229
                     /locus_tag="ACHKAY_004815"
     CDS             <1..1229
                     /locus_tag="ACHKAY_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5703338.1"
                     /translation="YLLNNMTVNDDANDVAVTESYKPIWNWKISQYNVFDIKFETMIK
                     PQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQL
                     LNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTFL
                     GDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDE
                     DTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELA
                     AIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVA
                     HNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKN
                     RNGLMPLIRRRTMLQL"
     gene            1419..1532
                     /gene="yhiY"
                     /locus_tag="ACHKAY_004816"
     CDS             1419..1532
                     /gene="yhiY"
                     /locus_tag="ACHKAY_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO5703339.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1595..2662
                     /gene="yhiI"
                     /locus_tag="ACHKAY_004817"
     CDS             1595..2662
                     /gene="yhiI"
                     /locus_tag="ACHKAY_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5703340.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWGRVNEELPWPDDLVVRLPQ"
     gene            2659..5394
                     /gene="rbbA"
                     /locus_tag="ACHKAY_004818"
     CDS             2659..5394
                     /gene="rbbA"
                     /locus_tag="ACHKAY_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO5703341.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5394..6518
                     /gene="yhhJ"
                     /locus_tag="ACHKAY_004819"
     CDS             5394..6518
                     /gene="yhhJ"
                     /locus_tag="ACHKAY_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO5703342.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1579 a         1653 c         1856 g         1606 t
ORIGIN      
        1 tttatttact taataatatg acagtaaatg atgatgctaa tgatgttgca gttactgagt
       61 cgtacaaacc catctggaac tggaaaatat cgcaatacaa tgtatttgat attaaattcg
      121 aaacaatgat aaaaccccag ttcgctgaca gaacctattt ctccaattgt tcacctgttg
      181 atcccacaag taccagacca acctattttg gcgataccga tggctcagtc ggtgcagttt
      241 tgtacgcgct ttttgcaacg ggacatttgg gaatcatggc tgaaggagaa aatttcctta
      301 gccagttgct taatatagaa gatgaagttc tcaacgtatt gctaagggag aactttaatg
      361 agcaacttga cactaatgtt aatataataa tcagtatcct gaacagaaga gacattgttt
      421 tagaaagtct tcagccttat ttggttatta ataaagacgc agtcacgcca tgtacgtttc
      481 tgggcgacca gactggcgat cgatttagta atatctgtgg cgataaattt atcatcgatt
      541 tattaaaacg cataatgagt atcaatgaaa acgttcatgt attagctgga aatcatgaaa
      601 cgaattgcaa tggtaactat atgcagaact tcacgcgcat gaaaccactt gatgaggata
      661 cctacgctgg gataaaagat tatcctgtat gtttctatgg ccccaaatac aaaataatgg
      721 caaatcatca tgggataact tttgatgatc agcgaaaacg ttacattata ggaccgataa
      781 cagtctctag tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatca
      841 ataaaaagca ccatgctgta attaatggca aaaagtttaa aaatagccgg gctatctctt
      901 gtcgatcctt taatcgttat ttttctgttt cgacagatta cagacctaaa cttgaagcac
      961 tattagcatg ctcacaaatg ttaggtataa atcaagttgt tgcacataac ggtaatggtg
     1021 gaagagaatg tataggtgaa acggggacag ttttaggtct taacgcccgt gacagtaaac
     1081 atgctggaag aatgttcagc atgcataatt gccaaattaa ccctggtgca gggcctgaga
     1141 taaccactcc ctggaagtct taccagcatg aaaaaaacag aaatggactt atgcctttga
     1201 ttagaaggcg cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta
     1261 tctggttgag actgttgata aaacgtaaaa aggaatgctc tgttcctgca actgaacagg
     1321 cgtgaaacgc gatccaacag gatggatacc gttttgtcag ccagttttat agatcacact
     1381 tatgagtatg cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg
     1441 ttttcactaa gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt
     1501 tccttcttat cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc
     1561 gtcgtttgct tacagggatt atggcgggcc gattatggat aagagtaagc gccatctggc
     1621 gtggtgggtt gtcggggcac tggcggtggc tgccgtcgtg gcgtggtggc tgttgcgccc
     1681 ggcgggtgtg ccggaaggct ttgctgtcag caatgggcgc attgaagcga cggaagtgga
     1741 tatcgccagc aaaattgccg ggcgtatcga caccattctg gtgaaagaag gccagtttgt
     1801 tcgcgaaggt gaagtgctgg cgaagatgga tactcgcgtg ttgcaggaac agcgactgga
     1861 agccatcgcg caaatcaaag aggcacaaag cgccgttgcc gccgcgcagg ctttgctgga
     1921 gcaacgacaa agcgaaagtc gcgccgcgca gtcgctggtt aatcaacgcc aggcagaact
     1981 ggactccgta gcaaaacgtc atatgcgttc ccgttcactg gcccaacgag gggctatttc
     2041 tgcgcaacag ctggatgacg accgcgccgc cgctgagagc gcccgagctg cgctggaatc
     2101 ggcaaaagct caggtttctg cttctaaagc cgctatagag gcggcacgta ccaatatcat
     2161 tcaggcgcaa acccgcgtcg aagcggcaca agccactgaa cggcgcattg ccgcagatat
     2221 cgatgacagc gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc
     2281 aggggaagtg ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta
     2341 tatgaccttc ttcctgccaa ccgaacaggc gggcacgctg aaactgggtg gtgaagcccg
     2401 gctgatcctc gatgccgcgc cagatctgcg tattccggcg accatcagtt ttgtcgccag
     2461 tgtcgcccag ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt
     2521 ccgcgtcaaa gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac
     2581 cggtttaccg ggcgtagcgt gggggcgggt gaatgaagaa cttccgtggc ctgacgacct
     2641 cgtggtgagg ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa
     2701 ctggcgggcg tgagccagca ttatggaaaa accgttgcgc tgaacaatat tactctcgat
     2761 attccggccc gctgtatggt cgggctgatt ggtccggatg gcgtcgggaa gtcgagctta
     2821 ttgtcgttga tttccggtgc ccgcgtcatt gagcagggca atgtgatggt gctgggcggc
     2881 gatatgcgcg acccgaagca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag
     2941 gggctgggca aaaatctcta ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct
     3001 cgcctgttcg gtcacgacaa agcggagcgg gaagtgcgaa ttaatgagct gctgaccagc
     3061 accgggttag caccgtttcg cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa
     3121 aaactggggc tgtgctgtgc gttaatccac gacccggaac tgttgattct tgatgagcca
     3181 acaacggggg ttgactcgct ctcccgcgcc cagttctggg atctgatcga cagtattcgc
     3241 cagcggcaga gcaatatgag cgtgctggtt gccaccgcct atatggaaga ggccgaacgc
     3301 ttcgactggt tggtggcgat gaatgccgga gaagtgctgg caaccggcag cgccgaagag
     3361 ttgcggcagc aaacgcaaag cgccacgctg gaagaagcat ttataaatct gttaccgcaa
     3421 gcacaacgcc aggcgcatca ggcggtagtg atcccaccgt atcaacctga aaacgcagag
     3481 attgccatcg aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac
     3541 gtcaatttcc gcattccacg cggggagatt tttggattcc ttggttcgaa cggctgcggt
     3601 aaatccacca ccatgaaaat gcttaccgga ctgctgcccg ccagcgaagg tgaggcgtgg
     3661 ctgttcgggc aaccggttga tccaaaagat atcgataccc gccgtcgggt aggctatatg
     3721 tcgcaggcgt tttcgctcta taacgaactc accgtgcggc aaaaccttga gttacatgcc
     3781 cgtttgtttc acatcccgga agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt
     3841 tttaagctca acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag
     3901 cggctttcgc tggcggtggc ggtgattcat cgcccggaga tgttaatcct cgatgaacct
     3961 acttctggcg tcgatccggt ggcgagggat atgttctggc agttgatggt cgatctctcg
     4021 cgccaggaca aagtgactat cttcatctcc acccacttta tgaacgaagc ggaacgttgc
     4081 gaccgcatct cactgatgca cgccggaaaa gtgcttgcca gcggtacacc gcaggaactg
     4141 gttgagaaac gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg
     4201 gcagggcaga gcaacgaagc cgaagcgccg cccgtggtac acgacatcac ccacgcgccg
     4261 cgtcagggat ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc actggaactg
     4321 cgccgcgatc cggtccgttc gacgctggcg ctgatgggaa cggtgatcct gatgctgata
     4381 atgggttacg gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac
     4441 cagaccgtca gtagccaggc gtggacgctc aatctctccg gttcccgtta ctttatcgaa
     4501 cagccgccgc tcaccagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg
     4561 gtggcaattg agatccctcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa
     4621 ctcggcgtct ggattgacgg agcgatgccc agtcgtgccg aaacggtaaa aggttacgtg
     4681 caggcaatgc accagagctg gttacaggat gtggcgagcc gacaatccac acccgccagc
     4741 caaagcgggc tgatgaacat tgagacgcgt tatcgctata acccggacgt aaaaagcctg
     4801 ccagcgattg ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc
     4861 gcccttagcg tggtgcggga aaaagagctt gggtcgatta tcaaccttta cgtgaccccc
     4921 accacgcgta gtgaattttt gcttggtaaa cagttgccat acatcgcgct ggggatgctg
     4981 aactttttcc tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt
     5041 ttcctgacgc tcaccctggc ggcgctgctg tatatcatca ttgccaccgg aatggggctg
     5101 ctgatctcca cctttatgaa aagccagatt gccgccattt tcggaacggc gattatcacg
     5161 ttgatcccgg cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggcct
     5221 ggacgttgga tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcgggacg
     5281 ttctcgaaag cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc
     5341 atcccgctgg tgatgggctt aagtatcctg ctgctgaaaa aacaggaggg atgatgcgcc
     5401 atttacgcaa tatttttaat ctgggtatca aagagttgcg cagtctgctc ggtgataaag
     5461 cgatgctgac gctgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta
     5521 cgccaggatc gttgaacctc gcgccgatcg ccattgccga tatggatcaa tcgcagttat
     5581 cgaaccggat cgttaacagc ttctatcgtc cgtggttttt gccaccggag atgatcaccg
     5641 ccgatgagat ggatgccgga ctggacgccg gacgctatac cttcgcgata aatattccgc
     5701 ctaattttca gcgtgatgtc ctcgccggac gccagccaga tattcaggtg aacgtcgatg
     5761 ccacgcgcat gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg
     5821 aagtgaacag ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa
     5881 cccggatgcg ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg atggcaatca
     5941 tcaacaacat taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg
     6001 aacacggcac ggtggaacac ttgttggtga tgccgataac gccgtttgag atcatgatgg
     6061 caaaggtctg gtcgatgggg ctggtggtgc tggtggtgtc gggattatca ctggtgctga
     6121 tggtgaaagg cgtgctgggt gtaccgattg aaggctcgat cccgctgttt atgctgggcg
     6181 tggcgctcag tctgtttgcc accacgtcaa tcggcatttt tatggggacg atagcgcgtt
     6241 caatgccgca actggggctg ctggtgattc tggtgctgct gccgctgcaa atgctttccg
     6301 gtggatccac gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgaccatgc
     6361 cgacgacaca ctttgttagc ctcgcgcagg ccatcctcta ccggggtgcc ggattcgaaa
     6421 tcgtctggcc gcagttcctg acgctgatgg caattggcgg cgcgtttttc accattgcac
     6481 tgctgcgatt caggaagacg attgggacaa tggcgtaaat acaattgccc gatgcgtaag
     6541 cttataggga ctacgcatcg gtgcaattta ttgaatttta gttgttttta ggccggataa
     6601 ggcgtttatg aactgacccc caaaacctgg acagtttcag ttaaccggct ttttgatact
     6661 cagtcctgta ttcagggcaa gattacgaaa gccc
//
LOCUS       DAZFKM010000208         6588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFKM010000208 DAZFKM010000000
VERSION     DAZFKM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2970
                     /gene="ehxA"
                     /locus_tag="ACHKAY_004820"
     CDS             <1..2970
                     /gene="ehxA"
                     /locus_tag="ACHKAY_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO5703343.1"
                     /translation="FNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYEAQGVGINEL
                     VKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDKLLQKYQKVG
                     SKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGEDISQNDIAKS
                     SIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNLPDLGSLGDG
                     LDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQYILAQRMAQG
                     LSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNYEGDALLAAF
                     HKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTISGILEASKQ
                     AMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFSRQHAVERAV
                     AITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQQVFDPQKGT
                     IDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWSVKGIKNHKG
                     VYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHDTVSYNKTDV
                     GKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQYRDFEFRTGG
                     IPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGNDRLYGDDGDD
                     YISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSGGTGDDKLYG
                     GGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLSDISFDDIAF
                     KRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVITDKDGREIK
                     VDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSGDERLASLYN
                     LSLHQNNTHSTTLTTTV"
     gene            3020..5140
                     /gene="ehxB"
                     /locus_tag="ACHKAY_004821"
                     /gene_synonym="hlyB"
     CDS             3020..5140
                     /gene="ehxB"
                     /locus_tag="ACHKAY_004821"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO5703344.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5144..6583
                     /gene="ehxD"
                     /locus_tag="ACHKAY_004822"
     CDS             5144..6583
                     /gene="ehxD"
                     /locus_tag="ACHKAY_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO5703345.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         2120 a         1035 c         1483 g         1950 t
ORIGIN      
        1 ttcaataatg cgacattgac tacaaaatca gcatttaata cagcatcatc aagcgtacgt
       61 tccgctggca aaaaactcat attattaata cctgataatt atgaagctca gggcgtgggt
      121 attaatgagt tggtcaaagc tgctgatgag cttggaatag aaatacaccg tactgaacga
      181 gatgatacag cgattgcaaa ccagtttttt ggtgcagcag aaaaagttgt aggattaact
      241 gaacgtggtg ttgcaatatt cgcaccacaa cttgacaaac ttctgcagaa gtatcagaaa
      301 gttgggagta aaataggaag aaccgctgaa aatgtaggta ataatctggg aaaagccgga
      361 acagttctct cagcactaca gaattttacg gggattgctt tatcaggcat ggctcttgat
      421 gaattgctga gaaaacaacg ggaaggagag gatataagtc agaatgatat tgccaaaagt
      481 agtattgaac ttattaatca gcttgtagat acagtatcaa gtataaacag taccgttgat
      541 tcattttctg agcagcttaa ccagcttggc tcatttttat ccagtaaacc tcgcttaagt
      601 tctgttggtg ggaaattaca aaatttacca gacctgggct ccctggggga tgggctggat
      661 gttgtctccg gaattctttc tgctgtatca gcaagcttta ttctgggaaa cagtgacgca
      721 catacaggaa caaaagctgc agcgggtatc gaactgacaa ctcaggttct tggaaatgtt
      781 ggtaaagctg tttcgcaata tattctggct cagagaatgg cacaggggtt atcgacaaca
      841 gctgcaagtg cgggtctgat cacatcggct gttatgctgg ctatcagtcc tctttctttc
      901 ctggctgctg cagataaatt tgagcgagct aagcagcttg aatcatattc tgaacgattt
      961 aaaaaattga attatgaagg ggatgcttta ctcgcagcct ttcataaaga aaccggagct
     1021 atagatgcag ccctgacaac aataaatact gtcctgagtt ctgtatctgc gggagttagt
     1081 gcagcctcca gtgcatccct cataggggcc ccgataagca tgctggtgag tgcattaacc
     1141 ggtacgatat ctggcattct ggaagcatca aaacaggcta tgtttgagca cgttgcagag
     1201 aaattcgctg ctcggatcaa tgaatgggaa aaggagcatg gcaaaaatta ttttgagaat
     1261 ggctatgacg caagacatgc tgcgttttta gaagactctc tgtctttgct tgctgatttt
     1321 tctcgtcagc atgcagtaga aagagcagtc gcaataaccc agcaacattg ggatgagaag
     1381 atcggtgaac ttgcaggcat aacccgtaat gctgatcgca gtcagagtgg taaggcatat
     1441 attaattatc tggaaaatgg agggctttta gaggctcaac cgaaagagtt tacacaacaa
     1501 gtgtttgatc ctcaaaaagg gaccatagac ctttcaacag gtaatgtatc aagtgttttg
     1561 acatttataa caccaacatt taccccagga gaagaagtta gagaaagaaa acagagtggt
     1621 aaatatgaat atatgacatc tcttattgta aatggtaagg atacatggtc tgtaaaaggc
     1681 ataaaaaatc ataaaggtgt atatgattat tcaaaattga ttcagtttgt tgaaaagaat
     1741 aacaaacact atcaggcgag aatgatttct gaactcggag ataaagacga tgtggtttat
     1801 tctggagcag gctcatcaga agtatttgct ggtgaaggtc atgataccgt atcttataat
     1861 aagacggatg ttggtaaact aacaattgat gcaacaggag catcaaaacc tggtgagtat
     1921 atagtttcaa aaaatatgta tggtgacgtg aaggtattgc aggaagtcgt taaggaacag
     1981 gaggtgtcag tagggaagcg aacagagaaa atacaatatc gtgattttga attcagaacc
     2041 ggtggaattc cttatgatgt aatagataat cttcattctg ttgaagagct cattggcgga
     2101 aaacatgatg atgaattcaa aggcggtaag tttaatgata tattccatgg cgcagatggg
     2161 aacgattata tcgaaggtaa ttatggtaat gatcgactat acggcgatga tggggatgat
     2221 tatatatccg gaggacaggg agacgaccag ttatttggtg gtagtggaaa cgataaattg
     2281 agtggagggg atggtaataa ttatctgaca ggaggaagcg gtaatgatga gcttcaggca
     2341 cacggagctt ataatattct gtcaggtggt actggtgatg ataaacttta tggtggtggt
     2401 ggtattgatc ttctggatgg aggggaaggt aatgactatc tgaatggtgg ttttggtaat
     2461 gatatttatg tttataggca aaactatggt catcatacaa ttgcagatga aggaggtaaa
     2521 ggagatcgtt tgcacttatc tgatattagc tttgatgata tcgcatttaa gagagttgga
     2581 aatgatctta tcatgaataa agccattaat ggtgtacttt catttaatga gtcaaatgat
     2641 gtcaatggga taacatttaa aaactggttt gcgaaagatg cctcaggagc agataatcat
     2701 cttgttgagg ttataacaga taaagatggt cgagagataa aagttgataa aatacctcat
     2761 aataataatg aacggtcagg ttatataaaa gccagtaata tagcatctga aaaaaacatg
     2821 gttaatatca ccagtgttgc caatgatatt aataagatta tttcttcagt ttcagggttc
     2881 gattcaggtg atgaacgatt agcatcttta tataatttat ccttacatca aaacaacaca
     2941 cactcaacaa ctttaacgac aactgtctga gactaaaaga ggaggtgatg gccattccag
     3001 gaggaatggt catcgttata tgatgagtaa atgtagttct cataatagtc tgtatgcact
     3061 gatattgctt gcacaatatc ataatataac tgtcaatgct gaaactataa ggcatcagta
     3121 taatacccac acacaagatt ttggggtgac tgaatggtta ctggcagcga aatccattgg
     3181 cttaaaagca aaatatgtag aaaaacattt ttccagattg tcaataattt ctttacctgc
     3241 gttgatatgg cgggatgacg gtaagcatta tatattgtct cgtgttacta aagattcatc
     3301 acgctatctt gtttatgatc cagaacaaca tcagtcacta acttttagtc gggatgagtt
     3361 tgaaaaactg tatcagggaa aagtcattct ggttacgtca agagcaacag tagtcggaga
     3421 gttagctaaa tttgattttt cttggtttat cccctctgtt gtgaaataca ggaggatttt
     3481 acttgaggtg ttaactgttt ctgcttttat tcagtttctt gcgttaataa cacctctttt
     3541 ttttcaggtt gtaatggata aggttttagt tcaccggggg ttttcaacgt taaatattat
     3601 cacaatagca tttattatag tgatactttt tgaagtgata ttaaccggag ccagaactta
     3661 tattttctct catactacaa gtcgtattga cgtcgaactg agcgctaagt tattcagaca
     3721 tttgcttgca ttgcctgttt catattttga aaatcgcagg gtcggagaga ccgttgccag
     3781 agtaagggaa ctggagcaaa ttcgtaattt tttaaccgga caagcgttga catcagttct
     3841 tgatctattt ttttctgtaa tatttttttg tgtcatgtgg tattacagcc ctcaattaac
     3901 actggttata ttattgtcac taccttgtta tgttatatgg tcattgttta tatcaccctt
     3961 attacgtcga cgtcttgatg ataagtttct caggaatgca gaaaatcaag cttttcttgt
     4021 cgaaacggta acagcaataa atacaattaa atccatggca gtatcaccac agatgattgc
     4081 tacatgggat aaacaactgg ccgggtatgt tgcttccagt ttcagggtaa atctggttgc
     4141 aatgacaggg cagcagggga tacagctgat acagaaaagt gtaatggtaa ttagtctatg
     4201 gatgggagca catcttgtca tatcgggaga gataagtatc gggcagttaa tcgcgtttaa
     4261 tatgcttgca ggtcaggtca ttgctcctgt tatcagactg gctcatcttt ggcaggattt
     4321 tcagcaggtt ggtatttctg ttgagcgcct tggtgatgta ttaaatacac cggtagagaa
     4381 aaagtcaggc agaaatatac tgccggaaat tcagggggat atcgaattta aaaatgtcag
     4441 atttcggtat tcttctgacg gtaatgttat tttgaataat attaatttat acatatcaaa
     4501 gggggatgtt atcggtatag ttggccgttc tggttcagga aaaagtacat taacaaaact
     4561 gcttcagcgc ttttatatac cagagaccgg acagatttta attgatgggc atgatttatc
     4621 acttgcagat ccagaatggt tacgacgcca gattggtgtt gtattgcagg aaaatatact
     4681 attaaatcgt agtattatcg ataatattac attagcttct ccggctgtat ctatggaaca
     4741 ggctattgag gcagccagac ttgcaggtgc ccatgatttt attacagaac taaaagaagg
     4801 gtacaatact attgttggag aacagggcgt tggtctttca ggagggcagc gccaacggat
     4861 cgcaatagcc cgggctcttg ttacaaatcc tcgaattctt atttttgatg aagcaaccag
     4921 tgctcttgat tatgagtcgg aaaatataat aatgaaaaat atgtcaagaa tatgtaagaa
     4981 cagaaccgta attattattg cacacaggtt gtcaactgta aaaaatgcaa acagaataat
     5041 tgttatggat aatggcttta tttctgaaga tggcacacat aaagagctta tctccaaaaa
     5101 agacagttta tatgcatatt tatatcagtt gcaggcataa cgtatgcgct tttacatgaa
     5161 aggtttatgg gatttggttt gccgttataa gacggttttt tctgatgtct ggaaaattcg
     5221 tcacacactt gatgctcctg tgagagaaaa agatgagtat gcatttcttc ctgctcacct
     5281 tgaactcatt gaaacaccag tatccagacg ttctcatttt gttgtatgga gtattttatt
     5341 atttgtaatt atatctcttc tcttatctgt tcttgggaaa gttgaagtgg tttcagtagc
     5401 aaatggtaag tttactcata gtggaagaag caaagaaata aaaccgatag agaatgcgat
     5461 tgtagaaaaa ataatggtaa aagatggctc ttttgtaaaa aaaaatgatc cattagttga
     5521 attgacggtg cctggtgttg aatctgatat tttaaaatca gaagcatctt tgttgtatga
     5581 aaaaacagaa caatatcgct acgcaattct ttctgaatca atacaacgga atgagcttcc
     5641 tgaaataagg ataacagatt ttcctggcgg agaagataac gcgggaggtg aacattttca
     5701 gagggttagc tcattaataa aagagcaatt catgacatgg cagaacagaa aaaatcaaaa
     5761 gcagctcaca ttaaataaaa aaatagttga acgggatgca gcgcttgctc gtgttagcct
     5821 gtatgagcat caggtatcac aggaaggaag aaaactcaat gattttaagt atttgttgaa
     5881 taaaaaagct gtttctcaac attcagttat ggagcaggaa aatagctata ttcaggcaaa
     5941 aaatgaacat gcagtctggc ttgcacaggt ttctcaactt gaaaaagaaa tagaacttgt
     6001 gcgggaagaa ctggcactgg agacgaatat cttcagaagt gaaattatcg agaagcacag
     6061 aaaatcaaca gataacattg tgttgctgga gcatgagctt gaaaaaaaca ggcagagaaa
     6121 agcatcatct tttattaaag ctcctgtgag tggtactgtt caggagttaa atatacatac
     6181 agaaggtggt gttgtcacaa cagcagaaac gctgatgatt attgtccctg ataatgatat
     6241 tctcgaagta acagcatctg tacttaacaa ggatatcggt tttatacaac ctggacagga
     6301 ggtcgttatt aaagtagatg catatccata tacacgtcat ggttacctta cagggaaagt
     6361 aaaaaatata actgcagatt ctgtttctgt tccggataca gggcttgtat ttaacgtgat
     6421 tatatctgtt gatcggaatg atatacaggg agaaagaaaa aaaatccctg ttacagctgg
     6481 aatgactgtt atggcagaaa taaaaaccgg ggtgcgtagt gttatcagct atcttcttag
     6541 tccattaaag gaaactatta atgaaagttt acgtgaacgt tagtatat
//
LOCUS       DAZFKM010000209         6483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFKM010000209 DAZFKM010000000
VERSION     DAZFKM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1036)
                     /locus_tag="ACHKAY_004823"
     CDS             complement(<1..1036)
                     /locus_tag="ACHKAY_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5703346.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFTWQF
                     LEQYPAAQDCAYKFLVCEDKPGMPRPAIDSWDTEYMQENRWDEESASFIPVEPESDPI
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     KAMYPERKLFAIGWVKHKCKPGTKWPEIQAELRNWKKRQDAERKETGKYTSVVDLARA
                     KANRQHTENPAEKIPPVTAAIHREYKQTWKTLDRELAYYLWPGDADAGNIDGSILRWA
                     KNEVIARDREDWKRISASMRKQPDALRYSRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEG"
     gene            complement(1130..1321)
                     /locus_tag="ACHKAY_004824"
     CDS             complement(1130..1321)
                     /locus_tag="ACHKAY_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5703347.1"
                     /translation="MNTTFALVLTVYLVSGESLDLVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(1318..1506)
                     /gene="dicB"
                     /locus_tag="ACHKAY_004825"
     CDS             complement(1318..1506)
                     /gene="dicB"
                     /locus_tag="ACHKAY_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO5703348.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            2074..2283
                     /locus_tag="ACHKAY_004826"
     CDS             2074..2283
                     /locus_tag="ACHKAY_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703349.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(2284..2922)
                     /locus_tag="ACHKAY_004827"
     CDS             complement(2284..2922)
                     /locus_tag="ACHKAY_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703350.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESMANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLEKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2934..3086)
                     /locus_tag="ACHKAY_004828"
     CDS             complement(2934..3086)
                     /locus_tag="ACHKAY_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5703351.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNTD"
     gene            complement(3352..3771)
                     /locus_tag="ACHKAY_004829"
     CDS             complement(3352..3771)
                     /locus_tag="ACHKAY_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703352.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            3871..4152
                     /locus_tag="ACHKAY_004830"
     CDS             3871..4152
                     /locus_tag="ACHKAY_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO5703353.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            4136..4561
                     /locus_tag="ACHKAY_004831"
     CDS             4136..4561
                     /locus_tag="ACHKAY_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5703354.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(4768..5223)
                     /locus_tag="ACHKAY_004832"
     CDS             complement(4768..5223)
                     /locus_tag="ACHKAY_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703355.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            5302..6417
                     /locus_tag="ACHKAY_004833"
     CDS             5302..6417
                     /locus_tag="ACHKAY_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5703356.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGP
                     DRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
BASE COUNT         1659 a         1433 c         1603 g         1788 t
ORIGIN      
        1 ttccttcatc ttcaaacacg ccctttgtag tcaggtattc agtaatgtat ttgttcagtg
       61 ccacagggtc tttgtgaatg tcgatcggac gttcacggac aaggccaaaa atagtctggc
      121 ggctgtagcg aagcgcatca ggttgtttgc gcattgatgc ggagatgcgc ttccagtctt
      181 cgcgatctct ggcgataact tcatttttag cccagcgaag gatgctgccg tcaatgtttc
      241 cggcatccgc atcaccaggc cagagatagt aggccagctc cctgtccagg gttttccatg
      301 tctgcttgta ttcgcgatga atggcggcag ttacaggagg gattttttct gctgggtttt
      361 cagtgtgctg tcggttggct ttggcgcggg caagatcaac aacagacgtg tattttccag
      421 tctctttgcg ctctgcgtcc tgccgttttt tccagttacg taattcagcc tgaatttcgg
      481 gccatttggt acccggctta catttgtgtt taacccatcc gatagcgaac agtttgcgtt
      541 ccggatacat agctttaatt tcaggcgttt tcatcagtgc ttcaacgata tgcccgtcaa
      601 aggtagccac gtcttcttgc agtaattcct gcgcgtcaat cgccatatca acggtgatgt
      661 tttcacatgt accgaactta accaggaccg cgttctgtac ttcaagggac agcttgtcaa
      721 aattgacgtt aataggatcg gattctggtt cgaccggaat aaaggaagcg gattcctcat
      781 cccagcggtt ttcctgcata tattcggtat cccaggagtc gatagcaggg cggggcatgc
      841 cgggtttatc ttcgcagaca agaaatttat aagcgcagtc ctgagcagca ggatattgct
      901 ccaggaattg ccaggtaaat ttggcacggg cgcggcgttc atcaccggct tcaatggcag
      961 tggctacagc aactgcgccc tcttctttta ttgcctgttc gtccggaatg gcggcgcaaa
     1021 taaagacttt actcattttg ttttaacctc attacagatt taagggtgaa caaatccctg
     1081 ccattgctgg catataaaaa tgaaaccgga tattaattac ggtgctgttt taaagtcctg
     1141 ccggtatttc gttattatta gtgtgagtag ttttatctac cggataacag ttaccgggaa
     1201 ttttttgttc tgctgctgca gccatgcatt ctttcattga accgtataag ccagtcacca
     1261 gatcaagaga ttcgccggaa acaagataaa ctgtcagaac gagtgcaaat gttgtattca
     1321 ttgtttatat cctttttgca gcaggtccag acgagccagc attgaaggaa tgcatacttc
     1381 atttaacagg tcctgctcga gttttctctg cttaatggcg tcttcaataa atgttttgtc
     1441 tccagtgata acgccaattt cgaaacgaag ttcagacgtg ctggcattac atgataactt
     1501 ttccattatc gcgtcctcaa caatgaattt tgtgatgcgg tgcctggtgc ctccaggtga
     1561 cgttaaccag ttaacaatta acgccggata cagagaatcc acccataaca ctgtttttgg
     1621 ttttaactgt tccgcgtgcg ctgagccgca ttcaccgcat cacaaaattc acttttaaaa
     1681 aagggcggca gagcagtcac ggagtaaaac tgataccgcc aaatgtcacc agaatattga
     1741 taacagaggg cgttgtagcg ggggtgtcac ttaagcgtat ggtcaacctg acaacccggt
     1801 gtcctcaact ggggaaggaa taaccccgcc atacttaccg ccgcgccatt tcgcggattg
     1861 ccacaaccgg aagcgcacgt tcgaagaaat ctaacgacaa gccttctaag ggaaagagct
     1921 tcgccgtacg ctttcgcgtt atgccctgac ttttcaggga aatatccttt cagtaaactg
     1981 tcagtaccgg attcttatcc gtgtccggcg cacgaccaca cgtgacagcg tgttggtctc
     2041 catttttaac ccagaacctc aatggaggat aaaatgccaa acaaaaaaag aaatccgctt
     2101 attgaaaaac agattgaatg cctggtaaat caactcaggc aatcagggtt attaaaaact
     2161 cattcagagt tgaggctcac agaatcagca ttcgacgata aattaaataa tgtcctttat
     2221 aatggcatta ttgattttaa tcgttctgtt ggtcgccgcg gccctgctgg tgtttcctta
     2281 taattaccag tcaatccaga gtggaccgtg ttcagcgtaa atataactgt acacatccag
     2341 attatatttg tggtctgtta agaacaggcc gcaaatacat gccgaagctt ccagtgcagc
     2401 ggctctgtta ctgaataacc atgtagcaac attccagcgt ttttctgcat cccagtcttt
     2461 ctcaaggcct gataccatga agaaaccgtt agtgttgcca tcaaataatt ctgtttccaa
     2521 atttttaagc aatgcctgat ggactcttgc caggtattcc gccggaattt cgccacgaat
     2581 tctaatgaga ttgtcataaa caaacatgtt ccccgcatat ggcgattttt ctttcttgtt
     2641 ttttaaacca gcatcatgag caaactgatc aatttcttct tccgttggtt tcgtattgat
     2701 gttttgcgct gtcgtttctg caattttatt tgccatactc tctgagtcgt gtttatttat
     2761 agacgcacag aaatacaatc cggtaaacgc atcgcgcaca ttacgagcca tattatcagt
     2821 gtcttttttc gttaccgatt ccaattcaag ttcgttcaga cgatgacgaa gtgtgtgtgc
     2881 tgcaatctcc tggattgaag gaggtaaatc tttaaattcc atcgtcaacc tcatcagtca
     2941 gtgtttctgg ctaaccagcg acgcgcgcca gcttcagttt taaacgtttt gcttttggta
     3001 tacgtcatcg cggtgaacgt accgtcctgg ttgggaaaca cgccgcatac cagagattcg
     3061 ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc cgggtagcgg
     3121 aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc atacgcctcg
     3181 ggctggctac ttaccccctg accactgcct ggtaactcga agtattgccc tgcgttctgt
     3241 gggacggggt gggttggtat gaaaggaagg atacccatgg gtatttaaaa agtaaatacc
     3301 catgggtaat ttttttgatg tgtcttaact ggtgactggt tgtttggtga gttatgatgc
     3361 gttttgtgct ttctttttac ggatttcttc gtagatcata ttgtaatact gttttttctc
     3421 ttcaagagtt tttaataagt tatccgcttc actttctggc agttcgtcta agagatctaa
     3481 aaaaatacgt tgtcgtggcg ttagaaccct tgtttcataa ttggaggctg tgttcgttga
     3541 tgatgaaacg ataccatcca tccatccccg gggtaaccca aaggactctt ctataatctc
     3601 caccatatca tcagcgatcc gtttttttcc ttttttcccc tctgggtaca acattcttga
     3661 tacataagaa ggctcgcgac cgatctttct ggccacgtta accgctttac catcgcattt
     3721 ctcatcacga attttgatga gttgttgtcg tctaaattca tatttgtcca taggtaaata
     3781 atagatgcga ttaccgcaag gtaaacaacc caagggtatt gacttttgtt tacctgtagg
     3841 tattcttgct gtgtttacta aggagtagct atggaagaat taagaatatt tctcaattct
     3901 ctttcgtcag atgaacagcg tatgtttgca tgtgagtgtg gtactagcat cggttatcta
     3961 agaaaggcat tgagtaaagg tcaaatgtta ggggcatcgt tatgtgtcct tattgagcga
     4021 gccagtaatg gtgaagttac acgtcagcaa ctaaggcctt ttgattggat gaatatttgg
     4081 cccgagctgg aagataccaa aacgttaaca caaccactta ctaggagctt gattcatgaa
     4141 aatcaagcat gaacacatcc gcatggcgat gaatgcctgg gcgcgtcctg atggcgaaaa
     4201 agttccagca gctggaataa cccaggctta ttttgagttg ggtatgacgt ttcctgaact
     4261 gtatgacgac agccatccgg aagccctggc tcgcaatacc cagaaaattt tccgctgggt
     4321 ggagaaagac actcctgatg cggttaaaaa aattcaggcg ttgttaccag cgatcgaaaa
     4381 agcgatgccg cctccgctgg tggcccgaat gcgcagccac agttccgctt attttcggga
     4441 gttggtagag acgaaggaac ggctggtgaa agatattgat gatttcgttg catcagcgat
     4501 cgttctgttc gatcagatga atcgtggtgg cccggcagga aacactctgg ctgtgcatta
     4561 attgggtaat aaatatgagt aatgacaaaa aattgacact gagcgtttac gaaaacagtc
     4621 cgcacatctg gcgtggcggt ttatctgatg tggagctggc agagtggttg atacataaag
     4681 ctaatgcgct gctctggcgt ttgtcagcca gagaacagcg caaggaaacc agaataaagc
     4741 tggctgatgc agaagcgtgt gccgggctta ttgaggatta tacaaatctt ggtatttctt
     4801 cagcagagag tgatcccatt cagcctctga gcagggagtc aatccagcac gctggttgta
     4861 tggcacatct tgtaactgct cgtcaacatg aggtgggtat tggatcactt ccggtgggat
     4921 attcgctgat tccagagctg gttgaagcaa gaaaatcagt tcagaaaaag agagatgacg
     4981 cacttcaatt attgagagag cactatggcg cgataccaga atgcgaacag catcgatacc
     5041 ctgaaggtta tgaatggatg cagtctcttt ttgaagttcg ctaatcaata tgtcgagacg
     5101 aaggtatgtt tcggcgcgca gccaggctct gtaatccggg agcatttcgg ggctgttaca
     5161 ccagcggttt gttgctgcaa catttaatac atgagcctga taaaggcttt tcaaaaaata
     5221 catgtcgaac ctcctctggt tctgtcgatt gggaaccaca gattatatcc ggaggaaggt
     5281 tcggcaccag atgaggtagc catgcgtgat tacgcaaaag tttctccgcg attctggctg
     5341 ggagaaacgg ggagagaact tagaaaggcg ggtgcagaag cgcaagttgt tgctttttac
     5401 ctgatgacat cccctcacgc aaatatgctg ggtttgtatt acctgccagt tttatacctt
     5461 gctcatgaaa ccgggcttgg tctggaaggg gcttcaaagg ggcttaaaag ggctgttgaa
     5521 gctggttttt gtagctatga ccatgatgca gagatggtct gggtccatga aatggcagcc
     5581 tggcaggttg gggaaacgtt gaagcctggc gataaccgtt gtgcaggtgt caggaatgag
     5641 tatgcatcat tacctgaaaa cgcttttctg tcagtgtttt acgacagata taaaacggat
     5701 ttccatctgg atgtgaggcg gaataatagc cgaaattcgg taaggggctt cgaaggggct
     5761 tttaaggggc ttcgaagcca agaacaggaa caggagcagg agaaagaaca ggaacaggac
     5821 aaaaacacta tggttcatgg caaaaaaaac accacgaacc aggcagggga tgttcagacc
     5881 gtcaatcctg gtcagccagc aggcacgaca ccggaagccg attcagcgta tgcgctgaaa
     5941 gccgattcgg gcgctgtgca gcaggtgatg accgcagggt cggagcaatc acaccaactg
     6001 cagcagcctg aagccgattc cgccattcag cgggaagccg atcgggtagt cccggaaagc
     6061 accgggcagt ctgtgggacg agtggattat ccggatgtgt tcgaacaggt ctggcgggaa
     6121 tacccgttgc gtgctggggc aaacccgaag aaatccgctt tcagtgcctg gaaggccaga
     6181 ttgcgcgagg gggtgccacc agagaccatg ctggatggtg tgaggcgtta cgcgagatac
     6241 ctggcggcga ccgggaaagc gggaacggaa tttgttcagc gagcgacgac gttttttggg
     6301 ccggaccgga attttgaaaa cccctggttg ctcccggtaa gcggcacgaa caaccagcgt
     6361 tgtgtgaatc atatttctga accggatacc gaaattccgc cgggattcag ggggtgatgt
     6421 ggcatgaaaa acattgcggc agccggggtt cttgaacgta ttcgcagact tgcaccacag
     6481 gcg
//
LOCUS       DAZFKM010000210         6389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFKM010000210 DAZFKM010000000
VERSION     DAZFKM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..364
                     /gene="ydjO"
                     /locus_tag="ACHKAY_004834"
                     /pseudo
     CDS             1..364
                     /gene="ydjO"
                     /locus_tag="ACHKAY_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(368..1759)
                     /gene="tcyP"
                     /locus_tag="ACHKAY_004835"
     CDS             complement(368..1759)
                     /gene="tcyP"
                     /locus_tag="ACHKAY_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO5703357.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1892..2482)
                     /gene="ydjM"
                     /locus_tag="ACHKAY_004836"
     CDS             complement(1892..2482)
                     /gene="ydjM"
                     /locus_tag="ACHKAY_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO5703358.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2645..3313)
                     /gene="hxpB"
                     /locus_tag="ACHKAY_004837"
     CDS             complement(2645..3313)
                     /gene="hxpB"
                     /locus_tag="ACHKAY_004837"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO5703359.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3460..3996
                     /gene="yniB"
                     /locus_tag="ACHKAY_004838"
     CDS             3460..3996
                     /gene="yniB"
                     /locus_tag="ACHKAY_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO5703360.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4037..4897)
                     /gene="yniA"
                     /locus_tag="ACHKAY_004839"
     CDS             complement(4037..4897)
                     /gene="yniA"
                     /locus_tag="ACHKAY_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO5703361.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5003..5293)
                     /gene="ghoS"
                     /locus_tag="ACHKAY_004840"
     CDS             complement(5003..5293)
                     /gene="ghoS"
                     /locus_tag="ACHKAY_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO5703362.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5394..6323)
                     /gene="pfkB"
                     /locus_tag="ACHKAY_004841"
     CDS             complement(5394..6323)
                     /gene="pfkB"
                     /locus_tag="ACHKAY_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO5703363.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1650 a         1597 c         1568 g         1574 t
ORIGIN      
        1 atacatcctg ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt
       61 tccttcgata atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg
      121 aatgggaagt ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg
      181 tctcgggtat gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat
      241 tgataagttg gacatttttt tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc
      301 cgcgatggaa catttaaaaa gtaaagcaac ggcccctata caaaacggac cgttgccagc
      361 ataagaatta acggtgtgcc agttcggcgt cgtcttcgct atccagaatg gctttatcgg
      421 tttgcttcag ccactggctg gtcagcgtgc cagctgtcat cgagccacta acgtttagcg
      481 ccgtacggcc catgtcgata agcggttcaa cggagattaa cagcgccacc agggttactg
      541 gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca ccgacaccgg
      601 caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac atcgggtcca
      661 gcgggttaat gccaaccgta ggcgcaacca tcaccgccag cattgccgga tacaaaccgg
      721 cgcagccgtt ctgaccaatg gttgcaccga aagaggcggc gaaactggcg atggattcag
      781 gaacgcccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca gcactggaac
      841 ggctggtaaa ggcaaacgtc agcacaggcc ataccttacg gaagtacttc agcggactca
      901 cgccattaat gcccagcaga atgccatgca ctgcaaacat aatcagcaga ccgaggtagg
      961 acgcgacaac gaaacttccc agtttgatga tgtcttgcag gttagaacct gcaaccactt
     1021 tggtcattag tgccagaacg ccgtaagggg tcaactgcat gaccaggcga accagtttca
     1081 tcacccagct ttgcagggta tcgatagcgg ttaagacgcg ttcacctttc ggcgcatcat
     1141 ccttcagcag tttcagcgca gctacaccga ggaatgcggc aaaaattacc acgctgataa
     1201 ttgacgtcgg attggctccg gtcagatcgg caaacgggtt tttcgggata aaggacaaga
     1261 ccagctgcgg aacgctcagg tcagagactt taccaacata gttactttcg atggcgttca
     1321 gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca aacaggttgg
     1381 tgaccagcac accgaccagc gccgcaatca gtgtggtaaa caaaagcgta ccgatggtca
     1441 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     1501 aggcgaagac taacggcata acgatcattt gcagcagttg aacatagccg ttaccaacga
     1561 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     1621 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     1681 actgtttgtg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     1741 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     1801 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     1861 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     1921 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaacc gcgctgtttt
     1981 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     2041 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cgacatggcc
     2101 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctt aaataaccca
     2161 gcaccattcc ttgtagcgca tcagccggaa tgaaccagct ttcaggaacc ttaaggtaaa
     2221 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     2281 cgcgggcgat cggttttgat atccacttta atcgctgccc aagaaacgac tttgggtgat
     2341 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     2401 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     2461 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     2521 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     2581 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgagatc actgcccctg
     2641 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     2701 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacaatg gaacgcatgc
     2761 gggctgcttt agaggcgatc atgccattta ctgaatcttc cagcgctacg caggtcagtg
     2821 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag
     2881 gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     2941 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     3001 tgcataacgc aacggcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg
     3061 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     3121 gccgggcgta ccaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct
     3181 cgttacgacg ggagatatcc acccccaggc ttgccatcac atccagttcg gctcgatccc
     3241 ataaaggttc tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac
     3301 gcggggttga cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct
     3361 tatgattaag cgcctatcat atcgccttta aagaatgggc gaaaacagat atctacaggt
     3421 aaactaagtg aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga
     3481 cgcattgctg ttttgaaaag gattttgggt tgggtgattt ttatacctgc attgatctct
     3541 acattgattt ctttgctgaa atttatgaat acccggcagg aaaaccagga aggcattaat
     3601 gcggtcatgc tcgactttac tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt
     3661 ttgaatctgt tctggtataa ctccccgacg cccaatttta atggtggcgt gaacgttatg
     3721 ttctgggtga ttttcatcct gatttttgtc ggactggcgt tgcaggattc cggtgcccgg
     3781 atgagtcgcc aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa
     3841 gccaaagggg aagaggggct aacgcgtgaa caaattgagt cccgtattgt tgttccgcac
     3901 catactattt tcctgcagtt tttctcacta tatatcctgc cggttatttg tattgctgca
     3961 ggttatgtgt tcttttctct gcttgggttt atttaatcaa aaggccgcac ggagcggcca
     4021 ttatcctcaa cccatatcat gctgctaata atctatccaa tgactgctga gcaataacca
     4081 aatgctgacc gccaaataat cttgcacgat ttaacagcgt gtagagttgg taaaccggtt
     4141 gacgttcaag gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg
     4201 gcggctgttc agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc
     4261 aggcagggtc gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat
     4321 cgccgtgcaa cagagaaggc tgcggttgat gcgaggccag acgctgctga atatgctcga
     4381 cgatggcgtc gatattgccg aaagcgatcc ctttctctgc tgccagttcc aactgccagc
     4441 caatccgttg ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag
     4501 ttgtggagag cgagttatca aaatcgaggc caaattgtgg ttggtcactc cattgatgta
     4561 aacgcgcaat ttgctgacca agaataaatg cgctatgcgc atccagcgga cgaggtggga
     4621 gataatccat caccagaaaa ctgtagtcac ggtcagcgcc tactgcccaa accttaggca
     4681 cggtgacggt tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaaccgg
     4741 gaagcagttc cctttcatca catttgacga aaaagtcatg tcctgcatag cgcaaatgcc
     4801 atgcggcatg gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta
     4861 actgctcgct caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc
     4921 cagatcataa ggttagcgca agaatgcggt gtaaaaacac gagctaacgc acaccacaga
     4981 ctgattattg cccaaaaacc atttattttc tgttgctatt ccattcctcc caggtcgtga
     5041 tggtgatttc aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa
     5101 tttcttcttc actttgggta ctgataagtc caaaagtgtt agtccccagc tcatggatat
     5161 ttccctcatc atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact
     5221 cgttaatttc agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat
     5281 cgccactggc catagttcac ccctttgtta tcacatgact tttgagcata gtcggagaaa
     5341 cgcgttgccg acaggttggt ggtgattccc ccaatgctgg gggaatgttt ttgttagcgg
     5401 gaaaggtaag cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg
     5461 agtgtggctg cactccccgc agctacgcca aaacggacca tctcttcaag agaggcattt
     5521 tctgccagtt tcagtgtcat cgcgccgacc atgctgtcac cagcgccaac ggtactctgg
     5581 cttttcaccg gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct
     5641 tgtggaccaa gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg
     5701 gctttgcgga catcgtctgg ctgggtgagt tcgcgattca ccagcgcact gagttctttt
     5761 tggttaggct taaccaactc gatgttacca atcgccagtg ctgcacttaa cgcttcgcca
     5821 gaactgtcga tgatgcagcg gatcccttgg ttttgcgcag cggaaatcag ttgggttaat
     5881 ttttccagct tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca
     5941 atttccagaa cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc
     6001 ataacaaaac gatactgctc accgcttgct tccacatgta cgtgtaaatt ctgccgggtc
     6061 cagtctttgg cttctacagt agcgacgggg acattttcat ccgccaacag agaaaccagg
     6121 tgttcgccgg tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca
     6181 atggcgcggg cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc
     6241 agttttcctt cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt
     6301 gtcaacgtat agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga
     6361 atcagccgaa agtgaagaaa ttaacaata
//
LOCUS       DAZFKM010000211         6146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFKM010000211 DAZFKM010000000
VERSION     DAZFKM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..852
                     /gene="exuR"
                     /locus_tag="ACHKAY_004842"
     CDS             76..852
                     /gene="exuR"
                     /locus_tag="ACHKAY_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO5703364.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1092..1175
                     /gene="yqiM"
                     /locus_tag="ACHKAY_004843"
     CDS             1092..1175
                     /gene="yqiM"
                     /locus_tag="ACHKAY_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO5703365.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1197..1859
                     /gene="yqjA"
                     /locus_tag="ACHKAY_004844"
     CDS             1197..1859
                     /gene="yqjA"
                     /locus_tag="ACHKAY_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO5703366.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1863..2246
                     /gene="mzrA"
                     /locus_tag="ACHKAY_004845"
     CDS             1863..2246
                     /gene="mzrA"
                     /locus_tag="ACHKAY_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO5703367.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2393..2761
                     /gene="yqjC"
                     /locus_tag="ACHKAY_004846"
     CDS             2393..2761
                     /gene="yqjC"
                     /locus_tag="ACHKAY_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO5703368.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2799..3104
                     /gene="yqjD"
                     /locus_tag="ACHKAY_004847"
     CDS             2799..3104
                     /gene="yqjD"
                     /locus_tag="ACHKAY_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO5703369.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3107..3511
                     /gene="yqjE"
                     /locus_tag="ACHKAY_004848"
     CDS             3107..3511
                     /gene="yqjE"
                     /locus_tag="ACHKAY_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5703370.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3501..3800
                     /gene="yqjK"
                     /locus_tag="ACHKAY_004849"
     CDS             3501..3800
                     /gene="yqjK"
                     /locus_tag="ACHKAY_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO5703371.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3986..4378
                     /gene="yqjF"
                     /locus_tag="ACHKAY_004850"
     CDS             3986..4378
                     /gene="yqjF"
                     /locus_tag="ACHKAY_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO5703372.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4448..5434
                     /gene="yqjG"
                     /locus_tag="ACHKAY_004851"
     CDS             4448..5434
                     /gene="yqjG"
                     /locus_tag="ACHKAY_004851"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO5703373.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5727..6092
                     /gene="yhaH"
                     /locus_tag="ACHKAY_004852"
     CDS             5727..6092
                     /gene="yhaH"
                     /locus_tag="ACHKAY_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO5703374.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1535 a         1521 c         1549 g         1541 t
ORIGIN      
        1 tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca
       61 ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgccgct
      121 gacctgaaag agcgcatcga acagggcgtc tatctggtag gtgataaact gcctgcagaa
      181 cgctttattg ccgatgaaaa gaacgtcagc cgtacggtag ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt cgaagtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagccgcgcc atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag
      421 gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag
      541 aactccgccc tggcggccat cgttgaaaaa atgtggaccc aacgtagcca taacccgtac
      601 tggaaaaaac tgcacgaaca cattgattcc cgtaccgtcg ataactggtg tgatgaccac
      661 gatcaaatcc tcaaggcgct gattcgcaaa gatcctcatg ctgctaagct ggcaatgtgg
      721 cagcacctgg aaaacaccaa gatcatgtta ttcaacgaaa ccagcgacga cttcgagttc
      781 aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac ggccaccagc
      841 ggcagtaaat gaaatacctg tccgacaggc gctgcgacgc gcctgtttgt ctcgtcggta
      901 agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcattcag
      961 caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa
     1021 ttagattcaa tttgaattta tgtttttgaa tgctttctta tctcacgatt taacagggaa
     1081 tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg
     1141 cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg
     1201 aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc
     1261 catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaacggct
     1321 tgcttccagc ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg
     1381 cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg
     1441 gctgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact
     1501 ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg
     1561 gtttatcggc gctgttaatt ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga
     1621 ttgccgggtt atcagggctg aataacgcgc gctttcagtt tttcaactgg atgagcggtc
     1681 tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg ccggtatttt
     1741 taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt
     1801 ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag
     1861 ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc
     1921 tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga
     1981 ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg
     2041 acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat
     2101 ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg
     2161 gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac
     2221 gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg
     2281 tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt
     2341 ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata
     2401 ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct
     2461 gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa
     2521 ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga
     2581 tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga
     2641 acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgataaga ttgccaaacg
     2701 cgaacggaaa ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta
     2761 actcacaata gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg
     2821 aacatctgcg tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat
     2881 ctggcgagaa gtcgaaagaa gagttgagta agattcgtag caaagcggag caggcactga
     2941 aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg
     3001 cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg ggcattggcg
     3061 ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca
     3121 tcacgcgcaa gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat
     3181 ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc
     3241 taatctcttt caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct
     3301 tatgagcctg atggtgctaa ttatttgggc ggttgatccg caatatcgcc tgaatgcgat
     3361 gattgccacc accgtggtgt tgctgctact ggcactaatt ggcggtatct ggacgttacg
     3421 taaatcgcgt aaatccacct tgctgcgcca tacacgccat gagctggcaa acgatcggca
     3481 actgctcgag gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac
     3541 tgcttagcca gatccagcaa caacggctgg atctttccgc cagtcgtcgt gaatggctgg
     3601 aggcaacagg cgcttacgat cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc
     3661 tggttggcag tagcgtgatg gcgatctgga cgattcgcca tcctaatatg ctggtccgct
     3721 gggccagacg cggttttggc gtatggagcg cctggcgtct ggttaaaacg accctcaagc
     3781 agcaacagct tcgcggttaa cttccctctg gccggagcaa ttccggcctt atccctcaaa
     3841 tttcttgaag atttttgaca gttttccttg ctaacaatca tcattcacca cgtttatgat
     3901 tctctccatc gacagcaacg acgctaatac cgcgcccttg cacaaaaaaa caatcagcag
     3961 cctgagtggc gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc
     4021 gcgcatttta atgccgattc tgtttattac cgcaggctgg ggaaaaatca ccgcttactc
     4081 tggtacccaa caatatatgg aagcaatggg tgtcccgggt tttatgctgc cgctggtgat
     4141 tctgcttgag ttcggtggtg gtctggcaat cctgttcggt ttcctgactc gcaccacagc
     4201 cctgtttact gcgggcttta cgctgctgac ggcattttta tttcacagca actttgctga
     4261 aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat tcctgctgct
     4321 ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc
     4381 acgcgctata cttaacgata aaaaacgagg aggaagctcc tcgtttttgc tattggagga
     4441 gagaaaaatg gggcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc
     4501 taccggcggt aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg
     4561 cgcacctggc cccactggca caggcggttt tatcgcagag aaagatcgtt atcatctcta
     4621 tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact
     4681 ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt
     4741 tgatgacagt tttccgggag caaccggaga tacgctctat caacatgaat ttctgtatca
     4801 gctttatctc cacgccgatc cacactacag cggacgagtt actgttcccg tgctgtggga
     4861 caaaaagaac cacaccatcg tcagcaacga atcagcagaa atcatccgca tgttcaatac
     4921 cgcgtttgat gcgctgggcg caaaagcggg tgattactac ccaccagccc tgcaaacgaa
     4981 aattgacgaa cttaacggct ggatttatga caccgttaac aacggcgtgt ataaagctgg
     5041 ttttgccacc agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc
     5101 gcgactggag cagattttag gccagcatcg ttacctgacc ggcaaccagc taaccgaagc
     5161 cgatattcgc ctgtggacca cgctggtccg ttttgatcca gtgtatgtga cccacttcaa
     5221 gtgtgataag caccgcatca gcgattacct taatctgtat ggcttcctgc gtgatatcta
     5281 ccagatgccg ggaattgccg aaacagtcaa tttcgatcac atccgtaatc attacttccg
     5341 cagccataag accatcaacc ctacggggat tatttcaatt ggtccatggc aggatctcga
     5401 tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag gcaccttacg aggtgcctct
     5461 gataattagc taagtgcatg ttgttcatgt cggatgcggc gtgaacgcct tatcaaacct
     5521 acaaaacatt tatattcaat atgttgcaat gaccgcgcag gccagataag cgtagcgcat
     5581 ctggcgattt tgcgcttgtc atcagtctta gcgcgccttc acagcacttt attaattcct
     5641 tgaacgaata tttactgcca tttcattcac gtctattctt aatttgctgc tttaaaaaca
     5701 agtgattgag caaaattgag gcaaaaatgg actggtatct gaaagtacta aaaaattatg
     5761 tcggtttccg gggtcgtgcg cggcgcaaag agtactggat gtttattctg gtcaacatca
     5821 tctttacgtt cgtgctgggg ctgctggata aaatgttagg ctggcaacgc gctggtggcg
     5881 aaggcatcct gacgacaatc tacggtattc tggtgttttt accgtggtgg gcagttcagt
     5941 tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc actgttgttc ttaatcccgt
     6001 ttatcggctg gcttattatc atcgtcttta actgccaggc gggtacgcca ggcgaaaacc
     6061 gctttggacc agatccgaaa ctggaacctt aaaacaaatc tgctcgccgg atgtctgact
     6121 catccggctt ttatcatcat gttgat
//
LOCUS       DAZFKM010000212         6131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFKM010000212 DAZFKM010000000
VERSION     DAZFKM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            111..245
                     /locus_tag="ACHKAY_004853"
     CDS             111..245
                     /locus_tag="ACHKAY_004853"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703375.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            846..1667
                     /locus_tag="ACHKAY_004854"
     CDS             846..1667
                     /locus_tag="ACHKAY_004854"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO5703376.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            1667..2773
                     /locus_tag="ACHKAY_004855"
     CDS             1667..2773
                     /locus_tag="ACHKAY_004855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO5703377.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            2867..4588
                     /gene="cptA"
                     /locus_tag="ACHKAY_004856"
     CDS             2867..4588
                     /gene="cptA"
                     /locus_tag="ACHKAY_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO5703378.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            4662..5660
                     /gene="lpxM"
                     /locus_tag="ACHKAY_004857"
                     /gene_synonym="msbB"
     CDS             4662..5660
                     /gene="lpxM"
                     /locus_tag="ACHKAY_004857"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO5703379.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLIAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            5713..5901
                     /locus_tag="ACHKAY_004858"
                     /pseudo
     CDS             5713..5901
                     /locus_tag="ACHKAY_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5964..>6131)
                     /locus_tag="ACHKAY_004859"
     CDS             complement(5964..>6131)
                     /locus_tag="ACHKAY_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5703380.1"
                     /translation="HGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
BASE COUNT         1558 a         1350 c         1555 g         1668 t
ORIGIN      
        1 tagcagcttt ttaaactaat atcgactttc aataaataag gacaggtaat ggacaacaaa
       61 aatatctgaa cccgggtgag attcataagt tgcacagtgc tgtcttcaaa gtgccacacc
      121 cggaacgtaa tcactgcctg ctccttatgg ggtatcttca tggtgtacaa gcatcagagt
      181 tattaggtat aaaactgtct gatattgatt tgcaggccgg taacctgaat atccggcggc
      241 tctaaacggt ttgtcaactc agcccctcgg ctgaaacagg aggtacagtc cattcgtcgc
      301 tggctgaaac ttcgggatga atgggtagaa gagggatatg aatggttatt taacctctat
      361 ggcaggtctg ctacaggtat tactgttgtt ctggggggca ctggtgtggt taatatctac
      421 gatgcaagag attctcacga tggcccggtc catcagtgca tgcggcgcga tattgtgcga
      481 gtatttttca gggggagtgg tatccggatg cagaaaattc cgctactcga ctggttttat
      541 gcaataaatc aggatgataa tcttctgatt ttttactgag gcggatatct ctcgctgtgt
      601 ttactgaaat tgcggtattc tgttttttct gaaaaacaga aaatctgatt aaaaagagca
      661 tatgcgttaa gggaaattta agttctggtt gattaactga taaaaatatt aaaaaatccc
      721 tgttgtcggt aatctccgga ccattccggg attacacaat aatatcacca tgtgtgatat
      781 ctatggatcc tcactgttgt ttacgcgagg atattttcag aatgcttaac agacgataat
      841 tttttatgtt gaatgcaagg catcttcccg tactgatgta tcatcatgtc agtcgttgtc
      901 cggggcttgt gacactctca cctgaaacat tttgtgaaca gatgaagtgg ctcgcagata
      961 ataactggcg gacggtaaca tctgctgagc ttgaatattt ttatcagggg ggaacgcttc
     1021 cacgaaagag tgtcatgctc acattcgatg atggttatct tgataactgg ctcagagcat
     1081 ggccggtcct gaaggaatat aaccttcatg cacatatttt tctcattacg gggctgattg
     1141 gtaagggaaa cgtacgtagc aggcaggagc aggaatattc tcaccgcgac tgtgaacggt
     1201 tgattgccga taaccgttca gatgaggtca tgcttcgctg gtctgaagtc cgggaaatgc
     1261 gtgattccgg actggttgaa tttcatgtgc acacccattc acataaacgc tgggacaggt
     1321 tgtctgtatc acgtgctgaa cagtgccggc tcatgaaaga agatattctg gtgggaaaac
     1381 aatgtctgac ggaaaagctg gggttctgta gttcccatct gtgctggcct gaaggatatt
     1441 ataatcggga ttatattaat ctcgccggta agcttggttt ctcttattta tatacaaccg
     1501 aaagaagaat gaactgtcct gaaaatggtt cattacgcat agggcgtatc agcaccaaag
     1561 agcgggaaca ttccggctgg ctgaaacgac gcctttttta ttatacaacg ccactttttt
     1621 cttcagtact ggctcttcat aaggggccaa gattaccaga taattaatga tgaaaatttt
     1681 attcactgaa tcttcgtcag atattggggg ccaggagctg caggcactgg cccagatgac
     1741 tgctcttcaa aagcagggac attccgtttt acttgcctgc agggaaaaaa gtaaaattgc
     1801 tccggaagcc agaaaaagag ggcatgatgt cacttttatt cctttcagga acagccttca
     1861 ccttccttcc atcctcagac ttcgacggat tatcggggaa tttaaacctg atctggtcat
     1921 ctgccatagt ggccatgaca gtaatatagc aggactgagc cgtctgatat gctgtcaccg
     1981 cttcagtatt gtcagacaaa aaacctacat cacccgtaag acccgaacgt tttccctgaa
     2041 ttatctgtgt gatttcattg ttgtgcctag ctcagccatg atggcgcatc tgatggcgga
     2101 aggtgtcagg acaccggtaa cggttattcc accgggtttc gactggcccg cattgcataa
     2161 tgaagccatg cgaccgttac ccctgcatat ccacgcctgg gctgcatcag cagacaatgt
     2221 tccgcttatc gttcaggtgg ggatgctacg tcccgaaaaa ggacatgaat ttatgctgcg
     2281 tgtattgtat cagctgaaga tggaagggaa atcatttcgc tggctcgtgg tgggcgcggg
     2341 gcgtgaagaa tacgaagcac gtctgcgtca gcagacagaa cacctgggaa tgagcggaga
     2401 tgtgttaatg gcaggtgccc ttttcccagc gttaccggtc taccggattg ccagtgtggt
     2461 ggtcatgcct tcggaaaatg aagcgtttgg tatggtactg gctgaagcgt cagtctctgg
     2521 cgtgcccgtt atcgccagtg agacgggcgg gatccctgat gtcattcaga agaatgtgac
     2581 agggacgctg ttacccgtgg gggatgtgtc tgcatggacc ggcgcactgc gcgatttttt
     2641 atcccgaccg gaacgcttcc ggatgatggc agccagcgcc agagaagata ttgagtaccg
     2701 gtttgatatt aacagaacgg cacagatcat tgtgtctctg gctagtcagg ctaaggggaa
     2761 atgtaacaga taacggcgga tcctcttttc agcccgaatt ttgttcagcg gttttccggc
     2821 aagtaacgga gtaatttatc acattgttta aatacgggga cggtcaatgc atttaaatac
     2881 cggacagaac aggcctactt tcagctggag tgcgctggga tgggcaattt tttattttgg
     2941 ctttttttcc actctcctgc aggtcatcat cttcagcagt gggtacagcg gaacgaatgg
     3001 aatacgggac tcactgttat tcagttgtct gtggttgatc ccggtgtttc tctatcctga
     3061 tcggataaaa ataattgcag ctgttgtcgg tttcattctc tggggcacgt cgctggcagc
     3121 actttgttat tattttctct atggtcatga attctctcaa agtgttcttt tcgttatgtt
     3181 tgaaacgaat gccagagagg ctggtgaata tttcagccag tatttcagcc ttaaactgtt
     3241 gcttatctcg ctggtatata ctgcggtgtc cgtttttctg tggacacgtc tgcgtcctgt
     3301 atatattcct ttgccatggc ggagaattgt ctctttcctg ctgctttatg ctctgcttct
     3361 gcatccggtt gttctgaaat cgttaatcag acaggagccg ctgaatgata ctctaggcaa
     3421 actggcatcc cgaatggagc ctgctgcccc ctggcagttt gtatccagtt attatcagta
     3481 ccatcagcaa ctgaatgcac tgacaacctt cctgaatgaa aatagcgcac tgccaccact
     3541 gggtaatctc agggatgaat caggggagag accacgcaca ctggtcctgg tgattggtga
     3601 gtcgacacag cgcgaacgca tgagcttata cgggtatcta cgtgaaacga cgccggagct
     3661 ggatgcactg cgtaaaaccg atccgggtct tactgtgttt aataatgtgg tggcatcgcg
     3721 tccgtacacc attgaagcat tgcaacaggc ccttactttc gccaacgaaa agaatcctga
     3781 tctgtatctg acgcagccgt cgctgatgaa catgatgaag caggcaggct ataaaacatt
     3841 ctggattacc aaccagcaga caatcacagc ccgtaacacc atgctcactg tattttcgcg
     3901 ccagacggac aggcagtact acatgaatca gcagcgaaca caaagtgcgc gtgaatatga
     3961 cactaacgtg ctgaagccgt tccgggaagt gctgaatgac cctgcaccaa agaaactgat
     4021 catcgtgcat ctgctgggta cgcacattaa gtataaatac cgttatccgg aaggtcaggg
     4081 gcggtttgat ggcattacag ggcatattcc cactggatta aatgcgaaag agctggaagt
     4141 gtataacgat tacgataatg ccaatctgtt taacgatcat gtggtggcca gtctgataaa
     4201 ggacttcaga gcaactgcgc cggacggctt tctgctttat ttttcagatc atggtgaaga
     4261 agtatatgat actccgccat ataaaacgca gggacggaat gaagataacc ccacacgtcc
     4321 catgtacact gttccgttcc tgctgtggac ctcggaaaag tggcatgctg cacatccgcg
     4381 agatttttcg cagtatgttg accgcaaata cagtctggct gaactgatcc acacctggtc
     4441 agatttggcg ggactgacat acgatggtta cgatccgacc cgttctctgg tgaatccgca
     4501 attcagggaa actacccgct ggattggaaa tccgtataaa aagaatgggc tcactgattt
     4561 cgacactctt ccgtatggtg agccatagta ttttcttatc ttcggttccg ttctgaaggc
     4621 aggaggatgt gttttcctct gctgtccggt ggactccagg gatggtacca ccttctgctg
     4681 tactctgtta tcataatgag ataagcaggc agataccggt aaatatgaaa aatatcagaa
     4741 ctgagtttat tccccgcttt aaccttacat tatgcttccc ccgttactgg atgacgtgga
     4801 caggtatcgg tattatctgt gtgtttgcaa tggtcccccc cgcactgaga gatccattgt
     4861 tgggaaaatt gggcatgctg gttgggcgtc tggggaaaag cgcacgtcaa cgggccttga
     4921 ttaatttatc gctttgtttt ccggagtaca gtgataaaga aaaggagaat atcgttgatg
     4981 ccatgtttgc aacagcctcc atggccgttg tgcttatggc tgaattagca ctgtccgggc
     5041 ctgataaaat ttcgcatcgt atacgctgga atggtctgga gattgtggag aagatggcac
     5101 agaacaatga aaaggtcatt tttcttgtgc cacatgcctg gggagtggat attcccgcaa
     5161 tgctgatagc tgcgtcgggc agaaaaatgg cagcgatgtt tcataaccag agaaatccgg
     5221 tggtggatta tgtctggaac agtgttcgtc gtcgtttcgg aggaaaactg cacgcacgta
     5281 atgacggtat tgcctccttt gtccgctcag tccggcaggg atactgggga tattatcttc
     5341 ctgatcagga tcatggcccg gaattcagtg agtttgcaga tttttttgcg acgtataaag
     5401 caactttacc ggttatcgga cggctcagca ggatcagtgg tgcacggata ataccgcttt
     5461 tccctgttta tgacggaaag acccatcatc tgactattca tgtcagcccg ccacttgcta
     5521 tacgacagaa gagtgatgct catattgcgc gacagataaa cgaagtggtt gaaaatttcg
     5581 ttcgtccaca tcctgagcag tatacgtgga tcctcaagtt gctgaaaacg cgtaaagaag
     5641 gggaagagga tccttactga ggcaggtttt tcaggataat gagcttaccc agcaatagtg
     5701 gacacacgct aagtgagtaa actctcagtc agaggtggct catatgacaa aaaaacagta
     5761 ccaaccagta aaaaaccccg taaatagcat tcgcctgaat ttcgcagtga agccctgaag
     5821 cttgctgaac gcatcggtgt tgctgtcgca gcccgtgtgc tcagtctgta tgaatcacag
     5881 cttgtaagcg gctcgtcaga actgtattga tatttactga gcggtcgtaa gcgtacagcg
     5941 agggccgtat tgacggggat gtgttattca gctggcagtg ctatgcgcca cggaagcagt
     6001 tcgctgaccc ggttgaccgg ccagtctgct atgacgccag gcacatggcg aaggtagctt
     6061 tctggatcca cgtcattcag tttgcacgtc ccgatcaggc tgtacagtag cgctccccgc
     6121 tcaccaccat g
//
LOCUS       DAZFKM010000213         5978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFKM010000213 DAZFKM010000000
VERSION     DAZFKM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..730
                     /locus_tag="ACHKAY_004860"
     CDS             <1..730
                     /locus_tag="ACHKAY_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5703381.1"
                     /translation="FNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDF
                     LNGDPDQPIIMGRTYHQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQV
                     YIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGS
                     LSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQ
                     VGESTVIRLSKDGITIVGGSVFIN"
     gene            758..1219
                     /locus_tag="ACHKAY_004861"
     CDS             758..1219
                     /locus_tag="ACHKAY_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO5703382.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1239..5975
                     /locus_tag="ACHKAY_004862"
     CDS             1239..5975
                     /locus_tag="ACHKAY_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO5703383.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQDERRLGIEYDHHNRPETLTDVM
                     GREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAETGAEGFRYDRHGNLLA
                     YTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVINPQGESYRYILDN
                     CGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETILYSRDAAGRLAEVITPEGKTQY
                     AYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDEHTVIRCILPPED
                     ERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQG
                     WNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYR
                     LDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETDQYAGGHRLK
                     QAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEYRHDAS
                     GRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELISQQFSRVRQ
                     AHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPD
                     PRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRY
                     VPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGKVREALDVHYEDLVRRKLGGIS
                     QEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKSTRTQIKKTIELAEEQGKEAQFW
                     FKYGVSPKVREYIESKGGKVILGMGN"
BASE COUNT         1463 a         1604 c         1843 g         1068 t
ORIGIN      
        1 gttcaactgg gaccgttata acccggcaga ccaagacagt tcgtgctgga tccgtgtggc
       61 acaggcgtgg gcaggcaccg gttttggcca cctggcgata ccgcgtgtgg gtcaggaggt
      121 gattgtagac ttcctcaacg gcgatccgga ccagccgatc attatggggc gcacctacca
      181 ccaggaaaac cgcccccccg gcagcctgcc gggaacaaag acgcagatga ccatccgctc
      241 caaaacgtac atgggcagcg gatttaatga gctgaagttt gatgatgcga cagggagaga
      301 acaggtctac atccacgcgc agaagaacat ggataccgaa gtgctcaacg accgtaccac
      361 caccgtaaaa cacgatcacc gcgaaaccgt aaaaaatgac cagacggtca cgatccagga
      421 aggtaaccgc cttcttacgg tggaaaaagg ccacaagatc accggagtac tgaaagggtc
      481 tttatctgag gatgtctttc aggacagagg cacgattgcc ggttcggtgc atgttgacgc
      541 tgtaaacaat ggtggcgaag gcgacggtat acaggcttat acggcgatta aggaaatttt
      601 gctggctgtg gaggaaagca aaattgcgct gacgccggat ggcattcagc tacaggtcgg
      661 ggaatcgacg gtaatcaggc tgtcgaagga tggcatcacc atcgtgggcg gttctgtttt
      721 catcaactga ttcactcaga ttatcaggga gtattttatg cagttcactt ttaatgaagg
      781 ccacattcaa ctgccgtctc aatggcagga tcagtcaatg caggtcctgg tgtccacaga
      841 taacagtggc atcaacctgg tcattacccg cgaagccgtt ccacagggaa ccctgacacc
      901 agagctgtac caggaaacgc tggcgctgta ccagggcaaa cttgatgggt acaccgaaca
      961 tgcctgccgg gaaatcactc tggcagaggc cccggcctgg ctgctggatt attcctggaa
     1021 cggtccggaa gatgagggga atcaggggcg gattagccag atagccgtat tccagcggcg
     1081 tggagatacg ctgctgacct tcactttcag cacctcctta tccctgaaga acagtcaaaa
     1141 gacgatgctg ctggaggtga taaagagctt caccccgttg ccaccggaga atgacatcca
     1201 aaaggaccag ccgcgctgac ataaacgagg gataacacat gagtgaagga ccaggcgggc
     1261 cacagggagc gaccgcaggc ggtacgctgg caatgcgaat gctgtcacag caggcgatgg
     1321 tcgccagcca gatgaaacgg gcagccaacg acaaagccat tgcacagatg ctggcagcaa
     1381 agaagtccgg cccacctgcc gccaggctgg gcgatgaaat tcagcataaa agttttctgg
     1441 gggcactggc aggggccgtg ctgggggcga tagtgaccat cgcagaaggt tgcctgatta
     1501 tggccgcctg cgccaccggc ccttatgcgc tggttctggt gcctgcgctg atgtatgcca
     1561 gctataaggc gagtgattat gtggaggaga aacagaacca gcttgaatca tggataaaca
     1621 gcttttgtga cacggacggc gccatcaata ccggttctga aaatgtaaac attaacggaa
     1681 agcccgctgc aagggccgcc gtcacccttc cccctcctcc cccacctgga gcaatacctg
     1741 aaatcccaca gggggaaccc tcatggggtg atattgccac tgacctgctt gaatcggcag
     1801 cggaaaaagc agtaccactg gcgaaggcct gggggaacgc tgttatcacc ctgacggaaa
     1861 gcaatgccgg ttttatggat cgcgtcagcg ccggcgcatc gcttctgttt cccgccggtc
     1921 cggtattaat ggagtttgcc accatggtgg gcgggcgtag cgaaatcaaa aaagatgtgg
     1981 atttcccgga agccggtgag gacacggcgc tctgcgacaa ggagaacaaa ccaccgagga
     2041 tagcccaggg cagtagcaac gtctttatca acaatcagcc tgccgcgcgc aagggcgaca
     2101 aactggagtg cagtgcggcg atcgtgggag gttcgccgga cgtctttatt ggcggtgaac
     2161 aggtgaccta tctggatatc cagccggagt tcccgccgtg gcagagaatg atcctggggg
     2221 gaataacgat agccagctat cttctgccgc ctgcgggact gctgggaaaa ctgaagaatc
     2281 tggcgagact cggcaaactg ggaaacctgc tggggaaaag cgggaagctg ctgggcgcaa
     2341 agctcggcgc gttgctgggg aaaacaggta agtcgttaaa aagtattgcc aataaagtca
     2401 tcagatgggt aacagatcct gtcgatccgg taaccggcgc atactgcgac gaacgtaccg
     2461 acttcaccct gggccagacc ctccccctct ccttcacccg tttccacagt tctgtactgc
     2521 cgctgcatgg cctgaccggc gtgggctgga gcgactcctg gagcgaatac gcctgggtgc
     2581 gtgaacaggg aaatcgggta gatattatca gccagggagc cacgctgaac ttcgccttcg
     2641 acggtgacag tgatacggcg gttaacccgt atcacgccca gtacattctg cgccgccgcg
     2701 acgattacct ggagctgttc gacagggatg cactgagcag ccgcttcttt tatgacgcct
     2761 ttccgggaat gcgtctgcgc cacccggtga ctgacgatac cagcgatgac cgcctggcac
     2821 acagccccga cgaccggatg tatatgctgg gcgggatgag cgacaccgcc agcaaccgca
     2881 tcacgtttga gcgcgacagc cagtaccgga tcacgggtgt cagtcacacc gacgggatcc
     2941 ggcttaaact gacgtaccac gccagcggct acctgaaagc cattcaccgc acggataacg
     3001 gcatacagac gctggcgacc tacgaacagg atgcgcgggg gcggctgaca gaagcggatg
     3061 cgcggctgga ctaccacctg ttttatgagt acgacgctgc ggaccggatc atccgctggt
     3121 ccgataacga ccagacgtgg agccgtttca cctacgatgc acagggccgg tgcgtgaatg
     3181 tcaccggggc ggagggctat tacaacgcca cgctggacta tggtgacggc tgcaccaccg
     3241 tgacggacgg caagggcact caccgttatt actatgatcc tgacggcaat attctgcggg
     3301 aagaagcgcc ggacggcagt accaccacgt atgaatggga tgaattccat cacctgctgg
     3361 cccgccactc ccctgccggg cgggtggaga agtttgaata caacgccgca ctcggtcagt
     3421 taagccgtta cacggcggca gacggcgcgg agtggctgta ccgctatgat gagcgcggcc
     3481 tgccggtaag cctggtgtcg ccacagggcg aagagacccg gctggcatac accgctcagg
     3541 ggctgctgtc ggggatattc cgccaggatg aacggcgtct gggcatagag tacgaccacc
     3601 acaaccggcc ggaaacactc accgacgtga tgggccgtga acaccacacc gaatacagcg
     3661 gtcacgacct gccggtgaag atgcgcggcc ccggcggtca gtcagtgcgg ttacagtggc
     3721 agcagcacca taaactgagc ggccttgagc gggcagaaac cggcgcagaa ggattccgct
     3781 atgaccgcca cggcaacctg ctggcgtaca cggacggtaa cggcgttgtc tggacaatgg
     3841 aatacggccc gttcgatttg ccggtggcgc gaacggacgg tgaaggccac cgctggcagt
     3901 accgctacga taaagacacg ctgcagctca cagaagtcat taacccgcag ggcgagtcat
     3961 accgttatat tctggacaac tgtggccggg tgacggaaga gcgtgactgg ggcggcgtgg
     4021 tctggcgtta ccgctatgac gctgatggcc tgtgtaccgc cagggtcaac ggcctggagg
     4081 aaaccatcct ctacagccgg gacgccgcag gccgcctggc agaagtcatc acgccggaag
     4141 gcaaaacgca gtatgcgtat gacaaatccg gcaggctgac gggtatcttc agcccggacg
     4201 gcacatcaca gcgcaccggc tatgacgaac gcgggcgggt gaatgtcacc actcagggcc
     4261 gacgggccat tgaataccac taccccgatg aacacaccgt tatccgctgt atcctgccac
     4321 cggaagatga acgcgacaga caccccgatg aatccctgct gaaaaccacg taccgctaca
     4381 acgccgccgg agaactgaca gaggtcattc tgccggggga tgagacgctg acgttcagcc
     4441 gtgatgaggc gggacgtgaa gtgctccggc acagtaaccg gggttttgcc tgtgaacagg
     4501 gctggaatgc agccggccag cctgtcagtc agcgcgccgg attttttccg gaggaaacca
     4561 catggggcgg gctgctcccc tcactggtac gggagtaccg ttacgacagc gcgggcaatg
     4621 tgtcggctgt caccagccgg gaagattacg gacgggaaac ccggcgggag taccggctgg
     4681 accggaacgg ccaggtcacg gcggtgacag cctcaggcac cgggctgggc tatggcgaag
     4741 gcgatgagtc ctatggctat gacagttgtg gctacctgaa ggcgcagtct gcgggcaggc
     4801 accggataag tgaagagact gaccagtatg ccggaggcca ccggctgaaa caggccggaa
     4861 acacgcagta tgactatgac gccgcaggcc ggatggtcag ccgcaccaga caccgtgacg
     4921 gctaccgccc ggaaacagag cggttccggt gggacagccg ggaccagctg accgggtatt
     4981 gcagcgcaca gggtgagcag tgggaatacc gccacgacgc cagcggcaga cgaacagaga
     5041 aacgctgcga ccggaagaaa atccgcttca cgtacctgtg ggacggcgac agtattgcgg
     5101 aaatccggga ataccgcgat gataaactgt acagcgtacg gcacctggtg tttaacggct
     5161 ttgagctgat aagccagcag ttcagccggg tacggcaggc gcatccgtcg gtggccccgc
     5221 agtgggtgac gcggacgaat catgcggtga gcgacctgac gggccgcccg ctgatgctct
     5281 ttaacagtga aggtaaaacc gtctggcggc cgggacagac cagcctgtgg gggctggcac
     5341 tcagcctgcc cgcagacacc ggctacccgg acccgcgcgg ggagtgggac ccggaagccg
     5401 accccggcct gctgtatgcg ggacagtggc aggatgcgga atcggggctg tgctataacc
     5461 ggttccggta ctacgagccg gaaaccggaa tgtacctggt gagtgatccg ctggggttgc
     5521 aggggggtga gcagacttac cggtatgtgc ctaatccact ggggtatatt gatccgctgg
     5581 ggctggcaaa aacttctgtt cccgcagaaa aaataagcct ttcggataaa gcaagagatc
     5641 tttttagaca aggaaaagta agagaagcat tagatgttca ttatgaggat cttgtacgaa
     5701 gaaagcttgg tggtatatct caagaaattg ccgggcgtga atatgatgtt gttactgaca
     5761 agatcatagc gcaggtcaag aggacttata gctctataga taatccaaaa aacttcttaa
     5821 gtaagtctac tcggacacaa attaagaaga caattgagct ggctgaagaa caggggaaag
     5881 aggctcagtt ttggtttaaa tatggagtaa gccctaaagt aagagaatat atagaaagca
     5941 aaggtgggaa agttatattg ggtatgggga attaacat
//
LOCUS       DAZFKM010000214         5929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFKM010000214 DAZFKM010000000
VERSION     DAZFKM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..886
                     /locus_tag="ACHKAY_004863"
                     /pseudo
     CDS             1..886
                     /locus_tag="ACHKAY_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(937..1051)
                     /locus_tag="ACHKAY_004864"
                     /pseudo
     CDS             complement(937..1051)
                     /locus_tag="ACHKAY_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1138..1614
                     /locus_tag="ACHKAY_004865"
     CDS             1138..1614
                     /locus_tag="ACHKAY_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5703384.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACMAQIARENGVND
                     DVIFKWLRLWQNEGRISRRLPVTTTSDAGVELLPVEITPDEPKEPMAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVKWHTEFGHLNRGDMLTSEQHRCSNEKKKF"
     gene            1595..2747
                     /locus_tag="ACHKAY_004866"
                     /pseudo
     CDS             1595..2747
                     /locus_tag="ACHKAY_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            2810..4259
                     /locus_tag="ACHKAY_004867"
                     /pseudo
     CDS             2810..4259
                     /locus_tag="ACHKAY_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4326..4818)
                     /locus_tag="ACHKAY_004868"
                     /pseudo
     CDS             complement(4326..4818)
                     /locus_tag="ACHKAY_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4741..4971
                     /locus_tag="ACHKAY_004869"
     CDS             4741..4971
                     /locus_tag="ACHKAY_004869"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5703385.1"
                     /translation="MNCWRCFPLFFIGILTTDEWGSHIRYVIEGDSSIFIQHIECMRI
                     LIKHLTRRAICFPHSVNFIKKHRYFIEKYMFY"
     gene            5086..>5929
                     /locus_tag="ACHKAY_004870"
     CDS             5086..>5929
                     /locus_tag="ACHKAY_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO5703386.1"
                     /translation="MIHPGSFLDKTINNTRGKNVNANIKHSQIQEIKRNFTYKKNDDI
                     SSRFKFYSSLVKQKNSTEDVVLIGKMISDEVRNYSTIHNDRNIIINSGNWKTSFLCNL
                     ARLLYSMFNGSVYFPSRDDENSSSPSPTLPTIHQSERKELSQQKSINHLKKSDTIDEY
                     IKIRKTRGIEDQITTTSQSLIINELLNGVNRNTIPFQKISELNDIIHSYENMQTKNSR
                     KGIEILVKQGELLSSLINYDKENKQLSDNISKIINLLGIEYQSHKVDIEPFIHAVWVA
                     GAPPE"
BASE COUNT         1741 a         1355 c         1483 g         1350 t
ORIGIN      
        1 attgtctcgc gtgatcgcag tggggtctgt gcgacagcag cacgtgaagg tgtaccacag
       61 gccagacagg tggtcgatgc cacctgctaa aaaatattgg cgatgcgctt gaacgaatga
      121 tgtacagaca tatacctctg atacgtcttg ttgccagcga gttgtcacca aagaaaccac
      181 ctgagccaga actgtctgag cctgcagcat cgctccgtcg tccggaacgc ctaaaacaac
      241 aaacccgcaa aaaacggcat cagcgttgga cagaggttat ggcccagcat aacaagggat
      301 gtagtttcag ggaaatatcc cgtattacag gcctgtcgcg tgtgacagtc agtcgctggg
      361 tgcgttcagg aacattccct gaaatgtcaa cccgacctcc aaagcgaggg cttctggacc
      421 catggaggga gtggttaaaa gagcaacgag aaagtggtaa ttataacgcc agccggatat
      481 ggcgggaaat ggtggcccgg gtaagccgct ggttgatgcc ctggagaata accagggatg
      541 aagaaaatta tgcttcccga tttattagtc tgatgtgtga aaagaacctg agctgaaaat
      601 agcgcagcaa ctggcactcg agttctaccg tattctgaaa acccaaaata aatcacagct
      661 aagtagttgg ttcactcgag tccacgaaag cggttcagca gaatttcggc gagtggcggc
      721 ggggatggaa gctgatgctg cagctatatg tgaggcaatc agtagtcgct ggagtaatgg
      781 tgttgtcgaa ggtcatgtaa atcgcctgaa gatgctgaaa cgccagatgt atggtcgagc
      841 aggatttgaa ctactcaggc agagggtaat gagtccactg gcatgaaaat ctccacccag
      901 gttgcggaag aggcactttt catccgtcgt tgacagctcc agcagatttt ctgacacagg
      961 caggctgtgt gcggtaaggg ctgaacttcc gggaagcctt tgccctcagt ccgtgacggc
     1021 acaggcttgc catcacggtt tttacgttaa aagtaagcgt aaaccgaccg cagtatgtag
     1081 ccattagaca agaattggta acttagacgc ccatctgaca cagacggaca tccaagtatg
     1141 gaattacagg actggcgaaa agaacctcgt aaaaagtatt cgaatgaatt caaacttcgt
     1201 atggttgaac tggcatcaca acccggtgct tgtatggccc agattgcacg agaaaatggc
     1261 gtcaatgatg atgttatttt caaatggctc agactctggc aaaacgaagg gcgtatatca
     1321 cggcgtcttc cggtgacaac cacttctgac gcaggcgttg aattattacc tgtagagata
     1381 acgcctgatg agccgaaaga accgatggct gctcttactc catccttatc cactcagact
     1441 actgtcagtg ccagctcctg caaagtggag ttccgtcacg gtaacatgac gctggaaaat
     1501 ccttcaccag agctgctcac tgtgaagtgg cacactgaat ttggccacct gaacagaggt
     1561 gatatgctca cctcagaaca acacaggtgc tctaatgaaa aaaagaaatt ttagcgcaga
     1621 gtttaaacgc gaatccgctc aactggttgt tgaccagaaa tacacggtgg cagatgccgc
     1681 caaagctatg gatgttggcc tttctacaat gacaagatgg gtcaaacaac tgcgtgatga
     1741 gcgtcagggc aaaacaccaa aagcctctcc gataacacca gaacaaatcg aaatacgtaa
     1801 gctgaggaaa aagctacaac gcattgaaat ggagaatgaa atattaaaaa aggctgccgc
     1861 gctcttgatg tcagactccc tgaacagttc tcgataatcg ggaaactcag agcgcattat
     1921 cctgtggtca cactctgcca tgtgttcggg gttcatcgca gcagctacag atactggaaa
     1981 aaccgtcctg aaaaaccaga cggcagacgg gctgtattac gcagtcaggt acttgagcta
     2041 catggcatca gccacggttc ggccggagca agaagcatcg ccacaatggc aacccggaga
     2101 ggctaccaga tgggacgctg gcttgctggc aggctcatga aagagctggg gctggtcagc
     2161 tgtcagcagc cgactcaccg gtataaacgt ggtggtcatg aacatgttgc tatccctaac
     2221 taccttgaaa ggcagttcgc cgtgaccgag ccaaatcagg tgtggtgcgg tgatgtgacc
     2281 tatatctgga cgggtaagcg ctgggcgtac ctcgccgttg ttctcgacct gttcgcaaga
     2341 aaaccagtgg gctgggccat gtcgttctcg ccggacagca ggctcaccat gaaagcgcgg
     2401 gaaatggcat gggaaacccg tggtaagccc ggcggggtga tgttccacag cgatcagggc
     2461 agtcattata cgagcaggca gttccggcag ttattgtggc gataccagat cagacagagt
     2521 atgagccggc gcggaaactg ctgggataac agcccaatgg aacgcttctt caggagtctg
     2581 aagagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
     2641 taacggacta tatcgttgga tattacagcg cactaagacc gcacgaatat aacggtgggt
     2701 tacccccaaa cgaatcggaa aatcgatact ggaaaaactc taactaggtg gccagttttt
     2761 gttgaccact tcagaaagcc agttccgtca gaaagaccag caactaagtc tggttgaaga
     2821 gacggaggcc ttcctgtgct ctgcactggc acgtgccgaa gaaaagatcg aagaagatga
     2881 acgggaaata gaacatctgc agactcagat agaaaaactg cgccggatgc tgttcggtac
     2941 ccgttctgaa aaactgcgtc gtgaagttga acaggctgag gccctgctga aacaacgcga
     3001 acaggacagc gatcgttaca gtgggcggga agacgctcca caggttcccc gccagttgag
     3061 acagccgcgc catcgtcgtc cgttaccggc acaccttccc catgcaatac accgtctgga
     3121 gtctgaagaa agctgttgcc cggagtgtgg cggtgagctg gattatctgg aggaagtcag
     3181 tgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg atccgcactg ttcgggtaaa
     3241 aaagcctgta caaaatgtga ctgcatcgtt gaagcaccgg cgccgtcccg cccgatagag
     3301 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacag
     3361 ctgccactgt atcgtcagag tgaaatcttt gcccgacagg gtgtcgaact gagccgtgca
     3421 ttactctcca actgggttga cgcgtgctgc cagttaatga ctccgctgaa tgatgccctg
     3481 taccgttatg tgatgaatac ccgcaagctt cacactgacg acacaccggt aaaggtactg
     3541 gcactgggcc tgaaaagacg aaaacagggc gcatctggac gtatgtccgg aatgatcgca
     3601 atgcgggttc gtcatctcct ccggcggtct ggttcgcgta ctcaccgaac cggcagggga
     3661 aacacccgga gcaacacctc cgccccctgc aggcggatgc gttcaacggt tacgatcggc
     3721 tgttcagtgc agaacgtgaa gatggtgcgc tgacagaagt tgcgtgctga gcccatgtcc
     3781 ggagaaaaat ccacgatgta tacatcagca gcaaaagtgc gacggcagaa gaagccctga
     3841 agcgcatcag tgaactgtac gccatcgagg atgaaatacg gggattacca gagtcagagc
     3901 gtcttgaagt caggcagcag cgaagcaaag cgttactgac gtcgctgcat gaatggatgg
     3961 tggagaagaa tggcacgctg tcgaaaaaat ccagaccagg cgaagcgttc agctatgtac
     4021 tgaatcagtg ggacgccctc tgttattaca gtgatgacgg tctggcggag gcggacaata
     4081 acacagcgga aagagcgctt cgtgccgtct gtctcgggaa aaagaattac atgttcttcg
     4141 gtagcgatca cggcggcgag cgtggagcac tgctgtacgg gctgatcggc acctgccgtc
     4201 tgaacggtat cgatccggaa gcgtatctgc gccatattct gagcgtacta ccggaatagc
     4261 cctccaaccg tgttgacgaa ctcctgccat ggaacgtagt actcaccaat aaataagcgt
     4321 caatacggtg ctccgtagcg ctgttttgac cggataaaag ccgcgagtac cacgctgtcg
     4381 cagtgctgac ggaactgctg acatgtactt atccttgtcc gccgctgaca ccacatatac
     4441 cagtcactgc ggccttcctg gagcacatgg cacattgcct tgatgttgaa cacagcctga
     4501 tgtttttcaa taaagacata cttcatttca ggcgcttcgc gaagtatgtc gcggcctttt
     4561 ggaggatggt cagctcttca tcccgttccg ccagctggcg tttgagacgt gcaacatcgg
     4621 tagacatctc cagctcacgt tcagaagaac gtctactgat tttgttgttg actacccagt
     4681 tgtagactgt gattcataca ggctgagctc acgggctgca gcagcaatac ctatactgtt
     4741 gtgaattgct ggcgctgctt ccctcttttt ttcattggta tattgaccac tgacgaatgg
     4801 ggtagtcaca tcagatatgt tatagaagga gactcatcta tttttattca gcatattgag
     4861 tgcatgagaa tattaatcaa acatctgacc cgtagggcta tctgtttccc gcactctgta
     4921 aatttcataa aaaaacaccg gtactttatc gaaaaataca tgttctacta attggatatc
     4981 accaaatcat ataaaataat gtgttttata taacaacctc aaaatacatt tgaggttgtc
     5041 accaatcaaa attcattaat tgttgcgtaa ctagagagta tacatatgat tcatccaggt
     5101 tcttttttag ataagacaat taacaataca agaggtaaaa atgttaatgc taatatcaaa
     5161 catagtcaaa ttcaagagat aaaaaggaat ttcacctata aaaaaaatga tgacatctca
     5221 tccagattta aattttacag ctcgctagta aagcagaaaa actcaacaga agatgttgtt
     5281 ttgataggaa aaatgatttc agatgaagtt agaaattaca gcaccataca taatgataga
     5341 aatatcataa ttaactctgg aaattggaaa acatcttttt tatgtaatct tgccagacta
     5401 ctatatagca tgtttaatgg tagcgtctat tttccttccc gtgatgatga aaatagctca
     5461 tcccccagcc caactctacc tactatacat cagtctgaaa ggaaggagtt atcacaacaa
     5521 aaaagcatca atcatttaaa aaaaagtgat accatcgacg aatacattaa aataagaaaa
     5581 acaagaggca ttgaagatca aataacaact acctctcaaa gtttgataat taatgagctg
     5641 ttaaatggag ttaatagaaa taccattcca tttcagaaaa taagtgagct caatgacatc
     5701 atacattcat atgaaaatat gcaaactaaa aatagccgaa aaggtataga aatattagtt
     5761 aagcagggag aactgttatc atcattaata aattatgaca aagaaaacaa acaattatca
     5821 gacaatatat ctaaaataat aaatttatta gggatagagt atcagtcaca taaagtagac
     5881 atagagccat ttatacatgc agtatgggtt gcaggtgcac ctcctgaaa
//
LOCUS       DAZFKM010000215         5899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFKM010000215 DAZFKM010000000
VERSION     DAZFKM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..1210
                     /gene="yiaY"
                     /locus_tag="ACHKAY_004871"
     CDS             59..1210
                     /gene="yiaY"
                     /locus_tag="ACHKAY_004871"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO5703387.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1375..2913
                     /gene="aldB"
                     /locus_tag="ACHKAY_004872"
     CDS             1375..2913
                     /gene="aldB"
                     /locus_tag="ACHKAY_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO5703388.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3132..3335)
                     /locus_tag="ACHKAY_004873"
     CDS             complement(3132..3335)
                     /locus_tag="ACHKAY_004873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703389.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            3458..3781
                     /gene="yiaW"
                     /locus_tag="ACHKAY_004874"
     CDS             3458..3781
                     /gene="yiaW"
                     /locus_tag="ACHKAY_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO5703390.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3787..4923
                     /gene="yiaV"
                     /locus_tag="ACHKAY_004875"
     CDS             3787..4923
                     /gene="yiaV"
                     /locus_tag="ACHKAY_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO5703391.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4920..5894)
                     /gene="yiaU"
                     /locus_tag="ACHKAY_004876"
     CDS             complement(4920..5894)
                     /gene="yiaU"
                     /locus_tag="ACHKAY_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO5703392.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1452 a         1414 c         1544 g         1489 t
ORIGIN      
        1 ttcgaatgaa aatcctcagt aagctgccca ccctttttta cactttcagg agtgtgttat
       61 ggcatcttca actttcttta ttccttctgt gaatgtcatc ggcgctgatt cattgactga
      121 tgcaatgaat atgatggcag attatggatt tacccgtacc ttaattgtca ctgacagtat
      181 gttaacgaaa ttaggtatgg cgggtgatgt gcaaaaagca ctggaagaac gcaatatttt
      241 tagcgttatt tatgatggca cccaacctaa ccccaccacg gaaaacgtcg ccgcaggttt
      301 gaaattactt aaagaaaata attgcgatag cgtgatctcc ttaggcggtg gttctccaca
      361 cgactgcgca aaaggtattg cgctggtggc agccaatggc ggcgatattc gcgattacga
      421 aggcgttgac cgctctgcaa aaccgcagct gccgatgatc gccatcaata ccacggcggg
      481 tacggcctct gaaatgaccc gtttctgcat catcactgac gaagcgcgtc atatcaaaat
      541 ggcgattgtt gataaacatg tcactccgct gctttctgtc aatgactcct ctctgatgat
      601 tggtatgccg aagtcactga ccgccgcaac gggtatggat gccttaacgc acgctatcga
      661 agcgtatgtt tctattgccg ccacgccgat cactgacgct tgtgcactga aagccgtgac
      721 catgattgcc gaaaacctgc cgttagccgt tgaagatggc agtaatgcga aagcgcgtga
      781 agcaatggct tatgcccagt tcctcgccgg tatggcgttc aataatgctt ctctgggtta
      841 tgttcatgcg atggcgcacc agctgggcgg tttctacaac ctgccacacg gtgtatgtaa
      901 cgccgttttg ctgccgcatg ttcaggtatt caacagcaaa gtcgccgccg cacgtctgcg
      961 tgactgtgcc gctgcaatgg gcgtgaacgt gacaggtaaa aacgatgcgg aaggtgctga
     1021 agcctgcatt aacgccatcc gtgaactggc gaagaaagtg gatatcccgg caggcctacg
     1081 cgacctgaac gtgaaagaag aagatttcgc ggttctggcg actaatgccc tgaaagatgc
     1141 ctgtggtttt actaacccga tccaggcaac tcacgaagaa attgtggcga tttatcgcgc
     1201 agcgatgtaa tcatcatttc cacaacggct ggcaaattgt cagccgcttt ttcaactatc
     1261 tctgtaaccc ttgcccgtaa attcgtgata gctgtcgtaa agctgttacc gactggcgaa
     1321 gatttcgcca gtcacgtcta cccttgttat acctcacacc gcaaggagac gatcatgacc
     1381 aataatcccc cttcagcaca gattaagccc ggcgagtatg gtttccccct caagttaaaa
     1441 gcccgctatg acaactttat tggcggcgaa tgggtagccc ctgccgacgg cgagtattac
     1501 cagaatctga cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc gggcaaacga
     1561 gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc gcacacctcg
     1621 gtgcaggatc gcgcggcgat tctgtttaag attgccgatc gaatggaaca aaaccttgag
     1681 ctgttagcga cggctgaaac ctgggataac ggcaaaccca ttcgcgaaac cagtgctgcc
     1741 gacgtgccgc tggcgattga ccattttcgc tatttcgcct cgtgtattcg ggcgcaggaa
     1801 ggtgggatca gtgaagttga tagcgaaacc gtggcctatc atttccatga accgttaggc
     1861 gtggtggggc agattatccc gtggaacttc ccgctgctga tggcgagctg gaaaatggct
     1921 cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac cccgctttct
     1981 gtactgctgc taatggaaat cgtcggtgat ttactgccgc cgggcgtggt gaacgtggtc
     2041 aacggcgcag gtggggaaat tggcgaatat ctggcgacct cgaaacgcat cgccaaagtg
     2101 gcgtttaccg gctcaacgga agtgggccaa caaattatgc aatacgccac gcaaaacatt
     2161 attccggtga cgctggagct gggcggcaaa tcgccaaata tcttctttgc tgatgtgatg
     2221 gatgaagaag atgccttttt cgataaagcg ctggaaggct ttgcactgtt tgcctttaac
     2281 cagggtgaag tttgcacctg tccgagtcgt gccttagtgc aggagtcaat ctacgaacgc
     2341 tttatggaac gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc actcgacagc
     2401 gtgacgcaaa tgggcgcgca ggtttctcac gggcaactgg aaaccatcct caactacatt
     2461 gatatcggta aaaaagaggg cgcagacgtg ctcaccggcg ggcggcgcaa gctgctggaa
     2521 ggtgaactga aagacggcta ctacctcgaa ccgacgattc tgtttggtca gaacaatatg
     2581 cgggtgttcc aggaggagat ttttggcccg gtgctggcgg tgaccacctt caaaacgatg
     2641 gaagaagcgc tggagctggc gaacgatacg caatatggcc tgggcgcggg cgtctggagc
     2701 cgcaacggta atctggccta taagatgggg cgcggcatac aggctgggcg cgtgtggacc
     2761 aactgctatc acgcttaccc ggcacatgct gcgtttggtg gctacaaaca atcaggtatc
     2821 ggtcgcgaaa cccacaagat gatgctggag cattaccagc aaaccaagtg cctgctggtg
     2881 agctactcgg ataaaccgtt ggggctgttc tgatataaga agctggtcgc attgggtatt
     2941 cattgcctga tgcgacgctt acgcgtctta tcatgcctac gggaacctga ccgtaggccg
     3001 gataaggcgt acacgccgca tccggcataa acaaagcgca cgttgttaca atttacgttt
     3061 taaacctacg tgcgcaataa cccagactac gccaacagaa gtgcgtttct ggggcgtgtt
     3121 ttgccaggag tttactctcg cgtttcgatg gtgagcgggc aaaccaggcc gtaaatcacc
     3181 attggcgttc cgaggccaat cagcaataaa cggtagataa ttgaaggttt aatacgcttt
     3241 atctgtactg tggatgtttt actggagtta ttcattagct tggtcaatag ttccattatt
     3301 tcaatatgat aaacctcgac ctgttgaaaa gccacgcagc acacttcaga aagtatataa
     3361 aaaacaatat gccgttatat aaataaggaa attgcactgc atcatgggat ttcataatat
     3421 gcgttaattg ttctttttta tattctctga gggaaatatg tttctggatt attttgcact
     3481 gggagtgctt atttttgtat ttctggtgat attctatggg attattattt tacatgatat
     3541 tccctacctg attgctaaaa aacgtaatca tcctcatgcc gacgccattc atgttgctgg
     3601 ttgggtgagt ctttttacgc tgcatgttat ctggccattt ttgtggattt gggccacgct
     3661 ttaccgcccg gagcggggat ggggaatgca aaaccatgac tcctccgttg tgcaactaca
     3721 acagcgtatt gccgggctgg agaaacagct cgccgacatc aaatcctctt ctgccgagta
     3781 atacttatgg atttactgat tattttgacc tatgtggctt ttgcatgggc aatgtttaag
     3841 atcttcaaaa ttcctgtaaa taaatggacc attcccacag cggccctggg tggcatattt
     3901 attgtcagtg gtttaattct gttaatgaac tataaccatc cgtatacctt caaagcgcaa
     3961 aaagcggtta tttctattcc tgttgtccca caggtgacag gcgtggtgat cgaagtgacg
     4021 gataagaaaa atacgctgat taaaaaaggt gaggtgctat ttcgactgga cccgacgcgt
     4081 tatcaggcgc gggtggatcg gctgatggcg gatatcgtta ccgcagaaca taaacagcgg
     4141 gcgttgggcg cagagttaga tgagatggcg gcgaatactc agcaggcaaa ggccacgcgg
     4201 gataaattcg ctaaagagta taagcgttac gcacgtggca gccaggcgaa agtaaacccg
     4261 ttttcagaac gcgatatcga tgtggcgcgg caaaattatc tggcgcagga agcctccgtg
     4321 aagtcatcgg cggcggaaca aaaacagatc cagagccagc tggatagcct ggtgttgggt
     4381 gaacattctc aaatcgccag cctgaaagca cagctcgcgg aagcaaaata taaccttgag
     4441 cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc aggtactgat tcgtccgggt
     4501 acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt ttatacccga tcagaaacga
     4561 caaatcgtgg cgcagttccg tcagaactcc ttgctgcgac tggctcctgg cgacgatgcg
     4621 gaagtggtgt ttaatgctct gccaggtaag gtattcagcg gtaagctggc agccattagt
     4681 ccagccgttc ccggcggagc ttatcagtcg accggcacct tacagacgtt aaacacagcg
     4741 ccgggttcag atggcgttat cgcgaccatt gaactggatg agcacactga tttgagcgct
     4801 ttaccagacg gtatttacgc ccaggtggcg gtttactctg atcatttcag ccatgtctcg
     4861 gtgatgcgca aagtgctatt acgcatgacc agctgggtgc attaccttta tctcgatcat
     4921 taatcttcgc tttccgccgc ttgttcaggc cgcagttgct gcgactgagc aagctggtgg
     4981 cgaatttcac gcagcatttc atccagcaca tactggaccc gccagggctg gtattcccgt
     5041 ttacggaaga tagccaccag atccagccac cattcatagg gttgctgaaa gcagggcacc
     5101 agcgtgccgt cttgcaggtc gctctgcaca cttttgtctg gcgcaaacac aatccccagg
     5161 tgatttcgtg ccagctccag cgcagattgc gtgttgtcgc aaacgtaatt tcccttcacg
     5221 cgataatccc gcacctcttt actcccggcg acgttgaagc gccagatatt cgcgtcgtcg
     5281 atcatcatcg agtcgatcaa aatacaggag tgatgttcga gttcgtcagg acggctaatg
     5341 ggatgtttct caagatagcg ctggctggca taagcggtta cggcatattg ggtaataaaa
     5401 ctggcaacca gcgattcatc ttttggtgga gcgtagctga ttaaaacatc acaatcatca
     5461 ggaaattcga cgccttcata aaaggcatta cgctcaagat tgcaggtttt tagcgataag
     5521 gtgatatcac cgatatcttt aattttatcg attacatgtt tggataaata ggtaattatg
     5581 ccggttgggg cgtagatggt gacccgacca cgtttctcat gcttataatc tgcaataaaa
     5641 ttattaagtt gctcgtttct gtccagcatg tcgttgatgt acggtagcag cgcggtgcca
     5701 aaaggtgtga gcatcagctg ccgggtggtt cggtcaaaaa cttttaaacc cacttttgat
     5761 tcaaaatcag caagatattt gctgacgttg gcctgtgcga tgccaagtac agtcgcggca
     5821 tggctgatat tttcactggc agcgattacc gagataattt ttaactcccg gtacttaagt
     5881 tgtaattttg tcatcgcaa
//
LOCUS       DAZFKM010000216         5879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFKM010000216 DAZFKM010000000
VERSION     DAZFKM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..288
                     /locus_tag="ACHKAY_004877"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHKAY_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKAY_004878"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHKAY_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO5703393.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHKAY_004879"
     CDS             complement(965..1639)
                     /locus_tag="ACHKAY_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO5703394.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKAY_004880"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHKAY_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO5703395.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKAY_004881"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHKAY_004881"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO5703396.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHKAY_004882"
     CDS             complement(3810..4130)
                     /locus_tag="ACHKAY_004882"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO5703397.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHKAY_004883"
     CDS             complement(4139..4441)
                     /locus_tag="ACHKAY_004883"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO5703398.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..5230)
                     /locus_tag="ACHKAY_004884"
     CDS             complement(4451..5230)
                     /locus_tag="ACHKAY_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO5703399.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            5611..>5879
                     /locus_tag="ACHKAY_004885"
     CDS             5611..>5879
                     /locus_tag="ACHKAY_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703400.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDI"
BASE COUNT         1322 a         1536 c         1585 g         1436 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaaaacgga
     5221 gatccagcat ggcctgccag ccttttttat tttgtggtaa cgcggtactg agcaatctta
     5281 cgcccctgat aaataaagat aaaacgacag taaagagccg gatttccttg ttatataaaa
     5341 gatgatgcct ttccagtgag ttttcgtctg aataatacga atgtttgttt tcgtcatgaa
     5401 gcgttccggc tcccggggct atacatcgcc agaaaagcaa tgcatattgt cgggattatc
     5461 cagagaatca ggcagaaaga tccgacaatg ggctgtgatt atgccatctg gctcagaatg
     5521 atatcaaggt gttatgggga taacttgctg atacaatagc agtatgacag attcattaat
     5581 gcggaaactg ctcactgaaa cagagtgttc atgactgagc actggtcggg tcatgcagat
     5641 gataatttcc ccttttcttg gggaagcgac agtattttca tgcatgcctg tcttcagtca
     5701 tacggggaga gcatctgtga tacagtccat acatttgttg cggacgtcgc tcctgagtca
     5761 ctgactggtc tgcaaacagt ttatctgtac acggcagggc ttctgtgtga tatcgttttt
     5821 catatcgagg gggggcctcc agagatgaat ggaataataa tatttcctga tgatataat
//
LOCUS       DAZFKM010000217         5761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFKM010000217 DAZFKM010000000
VERSION     DAZFKM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..354
                     /locus_tag="ACHKAY_004886"
     CDS             <1..354
                     /locus_tag="ACHKAY_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO5703401.1"
                     /translation="NGTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEP
                     LSAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMA
                     QMELVMKAAGFKEVK"
     gene            complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHKAY_004887"
     CDS             complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHKAY_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO5703402.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHKAY_004888"
                     /gene_synonym="yehT"
     CDS             complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHKAY_004888"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO5703403.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHKAY_004889"
     CDS             complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHKAY_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO5703404.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3534..3599
                     /locus_tag="ACHKAY_004890"
                     /pseudo
     CDS             3534..3599
                     /locus_tag="ACHKAY_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3827..4075
                     /locus_tag="ACHKAY_004891"
     CDS             3827..4075
                     /locus_tag="ACHKAY_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO5703405.1"
                     /translation="MRVESCIAKEKISKMPKGSVPALQQEMLRRIRKRYDNAEIIIKS
                     ASNNGLTILRATDKESAHEFVQETLQEARETADDWFIH"
     gene            4697..5488
                     /locus_tag="ACHKAY_004892"
     CDS             4697..5488
                     /locus_tag="ACHKAY_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avirulence protein"
                     /protein_id="HFO5703406.1"
                     /translation="MGHLERGLWKTETRWDGNDREQLGKEALGSEEPKPGSPFYGSRG
                     LKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQTGQVLAGRLKIQERVKFRADNRI
                     DAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYAAEHFGK
                     TWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRYDAGMEEKYVNTLDI
                     FTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
BASE COUNT         1651 a         1405 c         1288 g         1417 t
ORIGIN      
        1 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
       61 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
      121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
      181 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
      241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
      301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatcggtt
      361 ggcggtcatg ctctaaacat gaccgccaat tttttagcct tttttcacat gctggcgcgc
      421 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
      481 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
      541 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
      601 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
      661 tttttcataa atcaggccat tgaggaatga cgacaaaaaa atgtccggac aacgctgaaa
      721 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
      781 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
      841 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
      901 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
      961 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
     1021 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
     1081 tttccagagt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
     1141 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
     1201 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
     1261 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
     1321 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
     1381 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
     1441 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
     1501 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
     1561 gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
     1621 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
     1681 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
     1741 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
     1801 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
     1861 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
     1921 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
     1981 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
     2041 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
     2101 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
     2161 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
     2221 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
     2281 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
     2341 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
     2401 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
     2461 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
     2521 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
     2581 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
     2641 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
     2701 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
     2761 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
     2821 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
     2881 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
     2941 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
     3001 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
     3061 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
     3121 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
     3181 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
     3241 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
     3301 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
     3361 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
     3421 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
     3481 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
     3541 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
     3601 gacgcaacat ttttaaaaat caacgaagat cgtccaggag cgccgatttt caagggttgg
     3661 acagataatt cgttttcgat gtcgacgaac ttcgacggat ttcgcctata aaaagtgtcc
     3721 catacatgcc ccattgatgc cccatagaca atatcagcaa ttgacactgg ttatctatac
     3781 aactaaaaat aacacctgta cataaccaca gttacaggag ggtgttatgc gtgttgagag
     3841 ctgtatcgct aaagaaaaaa tcagcaaaat gccaaaaggt tctgttcctg ccctgcaaca
     3901 ggaaatgctg cgccgtatca ggaaacgata tgacaatgca gaaatcatca tcaaatctgc
     3961 cagtaataat ggactcacta ttttaagagc cacagataaa gagtccgcac acgaatttgt
     4021 acaggaaaca cttcaggaag ccagggaaac agcggacgac tggtttattc actgactaaa
     4081 atcccgtcca catgccggga atttcctatg tccgaagaaa atggtgtggt taactaagca
     4141 aaagtgtccc tttatcggta tagcaactca ttttgttaac atacggttgc aaaattgatg
     4201 acaagtgttt attttagcca caagaaatgt atgcaaacca gaattgggaa atgcatttat
     4261 aaaataagga atagcagata tgtttgcagc aaagcccgaa ttaagtcaaa gatgtatttc
     4321 tggactcctc acaactgggc caaatgatga aaacgtaaat aataaaccgt caagtgatat
     4381 gccgaacaga atcaaactag ttgtaagcga ttcacaaatc ggtatatata caactgacag
     4441 taatactctg aaggatggat tgtgtagcca agcccacata ggaactcaac cttatcggcc
     4501 aggtgctgac tcgccgataa ctgtgagatc attcctacaa atcacacttt ccaacaataa
     4561 ttacaaatca ttagagcatc tgcaaacatg agcagagaat tatctgaggt atcgcaaagc
     4621 agaagagaat attctgcgtt cgaccgttga ggggctaacc aatccagaat ctcctgtatt
     4681 caaacaaaca gcatggatgg ggcatctgga gcgtggcctt tggaaaacgg aaacacgctg
     4741 ggatggaaac gaccgcgagc agctaggcaa agaggctctt ggctcagagg agccaaaacc
     4801 tggttcacca ttttacggta gtcgtggact aaaattatcc gacagtgcgc attctgcctt
     4861 ctcaatgatg ctgtgcggtt ctgaaggtcc attcacaaaa gaacaagcac tctctggttt
     4921 cgagcttgct caaactggcc aagtgctggc tggaaggcta aagattcagg agcgagttaa
     4981 attccgcgca gacaaccgca ttgatgctca gcgtaacggt acacactcaa cccgcacacc
     5041 aacaggcatg gatctttctc aagatatcgg cacaattatg cgtgataaag ctgggctacc
     5101 ggtcatgagc ggaacatccg gttcctcctc tgatgccact cttgcaacac gttacgctgc
     5161 cgagcatttt ggtaagacat gggccgctcc aggcttaagc caagcagaag gatgcaaagc
     5221 tatctcggat ctgtcacatc actattttcg tgcagaaggc tccagtccac cacaatcaat
     5281 ggcaacggga ataaataagg ttcgttatga tgcaggaatg gaagagaaat atgtaaatac
     5341 actggatata tttacacata gctacccaga aatttatgct ggggttgcat tgacgattgc
     5401 tggggctggc ggtaatgatg agcaggcaat gtataatgtt acgcaagaag ccgctcgtat
     5461 tctgcacgaa gctgaaacta aagattgaac attctcaata aagagctgcg ccaggcagca
     5521 catattcagc attgcagtaa gatatgcatg cccagccccc tgtaaaccta taaatatttc
     5581 aaatcatatt gattatctta tagccatatt ttttatgaca ccctgtttca gtgaacatga
     5641 agacactcac cttgttcctg atattcatta gcatcatcct gattatttaa cttctctatt
     5701 cagggaagcc cgtctgatac aaagcttcta ttcctcgtta aatattctcc attctggcgc
     5761 c
//
LOCUS       DAZFKM010000218         5628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFKM010000218 DAZFKM010000000
VERSION     DAZFKM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            353..880
                     /locus_tag="ACHKAY_004893"
     CDS             353..880
                     /locus_tag="ACHKAY_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5703407.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPQ
                     QPSEPAYDFDDDIPF"
     gene            936..1169
                     /locus_tag="ACHKAY_004894"
     CDS             936..1169
                     /locus_tag="ACHKAY_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO5703408.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            1233..3191
                     /locus_tag="ACHKAY_004895"
     CDS             1233..3191
                     /locus_tag="ACHKAY_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO5703409.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3246..3680
                     /gene="psiB"
                     /locus_tag="ACHKAY_004896"
     CDS             3246..3680
                     /gene="psiB"
                     /locus_tag="ACHKAY_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO5703410.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
     gene            3677..4396
                     /locus_tag="ACHKAY_004897"
     CDS             3677..4396
                     /locus_tag="ACHKAY_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO5703411.1"
                     /translation="MSVHSRALVPLCTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPDDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLTGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREVA"
     gene            4396..4914
                     /locus_tag="ACHKAY_004898"
     CDS             4396..4914
                     /locus_tag="ACHKAY_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703412.1"
                     /translation="MQNARHPKTHRELCELACRFLRNNGFKVVFSDRFHAWTSCGEHP
                     DALGFRNGASCLIEVKCSRSDFLADRKKVFRSEPEKGMGDWRFYLCEPGVITVADLPA
                     GWGLLYAINGRVRKVHGWPGNHQWVVTVAKPFGANKQAECDYLFSALRRLEIRGVTEN
                     GADADRVQTYGL"
     gene            5032..5184
                     /locus_tag="ACHKAY_004899"
                     /pseudo
     CDS             5032..5184
                     /locus_tag="ACHKAY_004899"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            5129..5251
                     /locus_tag="ACHKAY_004900"
     CDS             5129..5251
                     /locus_tag="ACHKAY_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5703413.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
BASE COUNT         1256 a         1476 c         1755 g         1141 t
ORIGIN      
        1 cgcttcacgt cgttgcggcg ggtccctgcc cgttgttctg tccggtgcca ttcacctgtt
       61 taagcccctg tcgccggggg catagacagc cacaacctgc cgcactgact gtccagggta
      121 aatgaacagc ccacaaaatt ttttacccaa aacccggtaa aaaattttgc gttctgccca
      181 ggacaggtgc gtcaggccgt ggcagtgatg ccccttgcgg cgaaggttct caaacaggtg
      241 atggtgaata ccggagggaa caaaaggcag gtccccgcaa aacgtcaaaa ccgcggctcc
      301 cgaaactcaa cagatgatgg gggctgaaaa catcaacaga aggagacaca tcatggcagt
      361 tcgtggcatt aacaaggtca ttcttgtcgg tcgtctggga aaagatccgg aagtccgtta
      421 catcccaaac gggggcgcag tggcaaacct gcaggtggcc acgtcagaaa gctggcgtga
      481 caaacagacg ggggagatgc gggagcagac agaatggcat cgcgtggtgc tgttcggcaa
      541 gctcgcggaa gtggctggtg aatatctgcg caagggcgcg caggtctaca tcgaggggca
      601 actccgtacc cgtagctggg aagataacgg tatcacccgt tacgtcactg aaattcttgt
      661 taagaccacg ggcaccatgc agatgctggg acgtgccgca ggtactcaga ctcagccgga
      721 agaggcgcaa cagttcagcg gtcagcctca gccggaatca cagccggaac cgaaaaaagg
      781 tggcgcgaaa acgaaaggcc gtggacgtaa ggccgcgcag ccggagcctc agcagccgtc
      841 tgaacccgcg tatgacttcg atgacgacat tccgttctaa cgaaagacag tgacaaccgc
      901 cccgtccagt gcggggcatc accggagata tgaggatgag cgaatatttc agaatatttc
      961 aggggctgcc tgacggctcc tttacccgcg aacaggctga agccgttgcc gcacagtacc
     1021 ggaatgtctt tatcgaggat gatcagggaa cgcattttcg cctggttgtc cgcaataaca
     1081 gtggcctggt ctggcgtacc tggaattttg aggacggtgc cgggtactgg atgaaccatg
     1141 tcatccggaa tttcggcatt cttaagtaag agaggtgccg gacgggaacg cgtccggcag
     1201 acatacaacc atcccggtca aaggagaaat atatgtcagt tacagagtct aagacaaaaa
     1261 cggagcgtaa atccagccgt aaacctgcaa aaacgcagga aacagtcctg tctgccctgc
     1321 tggagcagac ggaggaagtg agcgtgtcgc tggcctcgct gattaaatca ccgctgaatg
     1381 tgcgcacggt gccgtattcc gcagagtccg tcagcgaact ggcggagtcc attaagggcg
     1441 ttggcctgtt gcagaatctg gttgttcatg ccctgcctgg tgaccgccac ggtgtcgccg
     1501 caggtggtcg ccgactggca gcactcaaca tgctggcaga gcgtggcatc cttccggctg
     1561 actggcctgt acgcgtgaag gttattccgc aggagctggc gacggctgca tcaatgactg
     1621 aaaacggtca gcgtcgggat atgcaccctg ccgaacagat tgccggattt cgtgcaatgg
     1681 cgcaggaagg caaaacgcct gcacaaatcg gtgatttgct gggctattcg ccccgccacg
     1741 ttcagcgaat gctgaaactg gcagaccttg cgcctgtcat cctcgatgcg ctggcagaag
     1801 accgcatcac cacagagcac tgtcaggcgc tggcgctgga gaacgacacc gcgcgtcagg
     1861 tgcaggtgtt tgaagccgcc tgccagtcgg gatggggcgg taaaccggaa gtgcagacca
     1921 ttcgtcgtct ggtgaccgaa agtgaagtgg cggtggcggg gaacagtaaa ttccgcttcg
     1981 tgggggctga tgccttctcg ccagacgaac tgcgcaccga tttgttcagc gacgacgaag
     2041 gtggctatgt ggactgcgtg gcgctcgatg ccgccctgct ggaaaaactc caggctgtcg
     2101 cagagttcct tcgggaagcc gaaggctggg aatggtgcgc cggacgcatg gagcctgtcg
     2161 gtgagtgccg tgaggatgcc ggaacatacc gctgtctgcc ggagccggaa gcggtgctga
     2221 cggaggcgga agaagaacgt ctgaacgaac tgatgacgcg ttacgacgcg ctggaaaacc
     2281 agtgtgagga atccgacctg ctggaagcag aaatgaagct gattgactgc atggcgaagg
     2341 tcagggcgtg gacgccggag atgcgtgccg gaagtggcgt ggtggtgtcc tggcgttatg
     2401 gaaacgtatg tgtccagcgt ggtgtgcagt tgcgcagtga ggatgacgtg gctgacgacg
     2461 ctgaccgtac ggaacaggtg caggagaaag cgtcagtgga ggaaatcagt ctgccactgc
     2521 tgacgaaaat gtcttccgaa cgcacgctgg cagtccaggc agcactgatg cagcaaccgg
     2581 acaaatccct gacactgctg gcatggacgc tctgcctgaa tgtgtttggc agcggagcgt
     2641 acagtaaacc agcacaaatc agcctggaat gtaaacatta ttcgctgacc agcgatgcgc
     2701 catcggggaa ggaaggtgcc gcattcatgg cgctgatggc agaaaaagcc cgtcttgctg
     2761 ccctgttacc ggagggatgg tcacgggaca tgacgacatt cctgtcactc agtcaggagg
     2821 tgctgttatc cctgctcagt ttctgcaccg catgcagcct taacggtgtc cagacccgtg
     2881 agtgtggtca cacgtcacgc agtccgcttg acacgctgga aaccgccatc ggctttcaca
     2941 tgcgagactg gtggcagccg acaaaagcaa acttcttcgg acacctgaaa aagccgcaga
     3001 ttatcgcagc cctgaatgat gccggactgt ccggtgccgc acgggatgca gagaagatga
     3061 agaaaggtga tgcggctgaa catgcagagc accatatgaa agacaaccgc tgggtgcctg
     3121 gctggatgtg tgcaccacat ccacagacag ataccactga acgcactgat aacctggctg
     3181 atgccgcctg atgaacaacc acaccgcccc gccggagacg gggcggcagc aagggagata
     3241 ccgtgatgaa aactgaactg accctgaatg tattacagac catgagcgca caggaatatg
     3301 aagatatccg ggctgccgga agtgatgaac gtcgtgagct gacacacgcc gtgatgcggg
     3361 aactggatgc gccggacaac tggacgatga acggcgagta cggcagcgag tttggcggat
     3421 ttttccccgt ccaggtccgt ttcacgcctg cccacgaacg ttttcacctg gcgttatgtt
     3481 caccgggcga tgtctctcag gtctgggtgc tggttctggt gaatgccggt ggtgagccgt
     3541 ttgcggtggt tcaggtgcag cggcggtttg caccggaagc cgtcagccat tcactggcac
     3601 tggcggcgtc actggatgcg caggggtaca gcgttaatga catcatccat atcctgatgg
     3661 cggaaggagg tcaggcatga gcgtacattc acgggcactg gttcccctct gcacagagca
     3721 acaggccgca tggcgggcgg tggcggagac ggaaaaacgc cgccatcagg gaaacacgct
     3781 ggcggaatat ccgtatgcgg gggcgttttt ccgctgtctg aacggaagcc gccggatttc
     3841 gctgtctgac ctgcgatttt tcatgccgtc cctgaccgca gaagaactgc acggcaaccg
     3901 tcttcagtgg ctgtatgcca tcgatgtgct gattgaaaca cagggcgagg tctgcctcct
     3961 tccccttccc gacgatgcag cagaacgctt gttcccgtcg gtgcggtttc gtgtcaggga
     4021 acgcagtcgc cataaatccg cactggtcat gcagaagtac agccgtcagc aggcgaggga
     4081 ggcagagcag aaagcccgtg cgtaccaggc gctggtcgct caggctgaaa tcgaactggc
     4141 attccattcg ccggaaacag tcggaagctg gcatgcacgc tggagtgacc gggtggcaga
     4201 gcatgacctg gaaaccctgt tctggcagtg gggcgagcgt tttccgtcac tgaccgggat
     4261 ggaacgctgg cagtggcagg atatgccatt ctggcaggtc atcgcagagg ctggtatggc
     4321 ggcaagggag gccggtcacg ctgtccgtga gatggagcgg tggatggtcc cgaacaaact
     4381 ccgggaggtg gcgtgatgca gaacgccaga cacccgaaaa cacacaggga actgtgtgaa
     4441 ctggcctgcc gttttctccg caacaacggc tttaaggtgg tgttcagtga ccgttttcac
     4501 gcatggacgt cctgcggtga acatcctgat gccctgggct tccgtaacgg agcgtcatgt
     4561 ctcattgagg tgaaatgctc acggtctgat ttcctggctg accggaaaaa ggtgttccgg
     4621 tcagagccgg aaaagggcat gggagactgg cgtttttatc tctgcgagcc gggggtgatt
     4681 accgtggccg atttaccggc gggctggggg ttgctgtatg cgattaacgg gcgggtcagg
     4741 aaagtacatg gctggcccgg aaatcatcag tgggtggtta cggtcgcgaa accgttcggg
     4801 gcaaacaaac aggctgaatg cgattatctg ttcagtgctc tgcgtcggct ggaaatccgg
     4861 ggcgtgacgg agaacggtgc agatgctgac agggtgcaga catatgggct atgatgtgcc
     4921 cggcgcttga ggctttttgc ctcatgacgt gaaggtggtt tgttaccgtg ttgtgtggca
     4981 gaaagcagaa agccccgtag ttaattttca ttaacccacg aggcccactg tatgtctaac
     5041 caacaccagt atggcctctt accgtgcaaa ttgcaaggag gagtaaacca tgaaactacc
     5101 gggagacgcc cttatctggt gcgtattaat cgtgtgctgc acgttgttga tattcacact
     5161 cctgacccgg aatcgcctgt gcgaagtccg gctgaaggac ggatacaggg aagttacggc
     5221 aacaatggct tacgaatccg gcggtaagta gcaacctgga ggcgggcgca ggcccgcctt
     5281 ttcagggctg atgctggtct gactgcactg aagcgccttt ataaaggggc tgctggttcg
     5341 ccggtggccc ctttctcctt gctggcattg tgcgggcatg aacaaccaga ctgccaggaa
     5401 ggccgtttcc ctccgggaac ggggaagaac gcgcgcctgc ggcgcccgtt cttttccccc
     5461 gccttctctg tttatgacct gtcagaaatc acatcccttc cctttgatta ttctcttttt
     5521 cctcctgttc tgactctgat tactgctgcc ctgattattg tctgttttgt tgttctcctg
     5581 gctgtgtctt ttccgggcgt ccccctggcg ggcttccatc cctgacgc
//
LOCUS       DAZFKM010000219         5227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFKM010000219 DAZFKM010000000
VERSION     DAZFKM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..833)
                     /locus_tag="ACHKAY_004901"
     CDS             complement(<1..833)
                     /locus_tag="ACHKAY_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5703414.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     T"
     gene            complement(835..1107)
                     /locus_tag="ACHKAY_004902"
     CDS             complement(835..1107)
                     /locus_tag="ACHKAY_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703415.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1054..1233
                     /locus_tag="ACHKAY_004903"
     CDS             1054..1233
                     /locus_tag="ACHKAY_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703416.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(1275..1430)
                     /gene="hokD"
                     /locus_tag="ACHKAY_004904"
     CDS             complement(1275..1430)
                     /gene="hokD"
                     /locus_tag="ACHKAY_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5703417.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1988..3085
                     /locus_tag="ACHKAY_004905"
     CDS             1988..3085
                     /locus_tag="ACHKAY_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO5703418.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRD
                     MEIRVIPVSSPTRPSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINHLGIPLSNI
                     DIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVGL
                     YSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDF
                     WVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGN
                     YKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
     gene            complement(3045..3623)
                     /locus_tag="ACHKAY_004906"
     CDS             complement(3045..3623)
                     /locus_tag="ACHKAY_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO5703419.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(3929..4240)
                     /locus_tag="ACHKAY_004907"
     CDS             complement(3929..4240)
                     /locus_tag="ACHKAY_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703420.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(4233..4487)
                     /locus_tag="ACHKAY_004908"
     CDS             complement(4233..4487)
                     /locus_tag="ACHKAY_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703421.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(4484..4906)
                     /locus_tag="ACHKAY_004909"
     CDS             complement(4484..4906)
                     /locus_tag="ACHKAY_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5703422.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            complement(4947..>5227)
                     /locus_tag="ACHKAY_004910"
     CDS             complement(4947..>5227)
                     /locus_tag="ACHKAY_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703423.1"
                     /translation="DGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDS
                     FWVKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
BASE COUNT         1363 a         1243 c         1115 g         1506 t
ORIGIN      
        1 cacgtcatgc atttctgcgt ttttacccaa cgcgtgtata caggcatctc ttcccgtcgt
       61 ggtttctggc gctggagata ctgagccggt gactccggat caacggcaat gcttaccacc
      121 gtcttttcct gtggcgggtt ttgctggtgg gcgtgaggca gtagcgcaat attttttgtg
      181 cgctgcttca gcatgctggt ggcggtctgc tctcccggca cgatgtcgct ctcgcggtat
      241 actgagcgaa ttttttccgc gcgtaatccc agtgaacgac gtaacaccgt ctccggtagt
      301 gcgtccgcta cgttatttat ggttgcccac caggataatt cagccagcga taattcccgc
      361 tcctgcgtgc cattcattgc gtggcggatg acatcaatca tccatgctga caggttttgg
      421 tgagcaagct gcccgagtga ttcggaagtc tggttacgca gctggttgtc gcagtgccag
      481 cacaacacca tcgcgccggt accgtaacga tgtatgacgg tttcactgtg atggtagtca
      541 ccatgaggcc actggcagga tttaatgtgg cgtaacagcc agtcagacaa tgcaccagca
      601 ccaccagcag cacgaatcac ccgctcatcg ctgaaaaatg gcagtaatga tttatcctcc
      661 gccagcggct ggcgaacagc aggaacgact ccggacggca gaccgcgcat gcttttcggt
      721 tccggctcca ccagaactcg agggttatga aatacctgca tggattcacg gcccggtttt
      781 agcaccacca gcccaagttc cggtaccgga acaggtcgaa gtaatacccg cacgttacct
      841 ccagatgcgt tgctggaatg tgcgggacgg acgcggtggg cgttcggaat aagggagcct
      901 gacatagatt atccagtgac gataatcgag gctgagggct ttcttaatct cgtatccgcg
      961 tctgcggtag ttatgaatta gccattcggc ctgttcttca gtacatggtg ggtgttggta
     1021 ccagtcggtt ttaaatgcgt gtgaacgccg cccatgccgg atggcaaggt cggtatcaga
     1081 attgtgaaat ttggttttgt gcaccatctg ttttctctgc tggcgcagca ggtgtcaggt
     1141 gttcaggctg acgtgcgaat tgtaaaccag aatgccagga aaaaacaaaa cccgccgaag
     1201 cgggttaagt gcgggtgcgt tgaggatgcc tgactcatca gaggtggcga gggatttctc
     1261 cctcgcctgg tctcttactc ctcaggttcg taagctgtga agacagcgac ctccgtctgg
     1321 ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt cactatgacg
     1381 gttaaacaga tgacgatcag ggcgattaac atcgcctttt gctgcttcat agcctgcttc
     1441 tccttgcctt tcggcacgta agaggctaac ctacatgtgc aaagcatgaa attggcctca
     1501 gattaatgtt aagcgtcttg ccggacgcgt aatgttaact ggggcttttc tctatctgcc
     1561 ttttggtgtt catgcctgag gcagatagcc tcaagcaccc gcagcaattc taactatcca
     1621 atagatcaat gccaacttct tttcctgcaa catctctcat cagaagtgac ccaaattcat
     1681 ccaatcagca caaaagaaac atttaaagcg tgaatgctac cgaacgttca tttcaattac
     1741 atcgttttga tttttaacag ttttcacctc aacaaatcaa atggcattag cattcatcat
     1801 ggaagaaaga ctgataaaaa tgatcatttt caatgactta tgatcacacc gggtattgcc
     1861 aagtcattcc ttttacgcca ggatacccac aagtgagtag tcgaggagag tttgccaatt
     1921 tttgcgatat aaatttcaat gtccgaagaa ccattaattg ttaattgatt ctgtataagg
     1981 tattaacatg acaatttcat atattcctat tctgaaagcg aaacgttctg agttatcagc
     2041 tttatcgcag ctatccattg aaaagaaatc aaaaattttg ccattactcg aaattgaacc
     2101 agtgccaatt gaccctgatt caggtattgc cttaaagagt tataacgaga ctcttataga
     2161 gttcggaaag aaagtctcaa aatcttgctc agatatgcaa ggtgtttata ttgatggatt
     2221 attaattgaa gagcatttta tttctcctga agatcattac cctataataa atgcggttaa
     2281 tcaagttaga gatatggaga taagagttat tcctgtcagt tcaccaactc gcccatctaa
     2341 ctataaaaga gcgattgatg aattaatgca gaatgaaata tgcttgagat taaccacgtt
     2401 agatctggtt aacccacaat taataacgca ttacattaat catctgggaa ttcctttatc
     2461 aaatattgat ataattattg acttaagaga tgagttaacc gaggataaaa ttaattccgg
     2521 cgaactatat actttggcaa tgggattgat aaacaatctg gcgcacctca atgaatacag
     2581 aaaagtgatt ctttctgggg gttcatttcc tacagatctc agtgatattt ctgttggtct
     2641 atattctcaa cctcgaatcg agtggatttt atggcagagt ttaatgaata gaaaagaact
     2701 tgccagaaat gtgatttata gtgattatgg agtacagcac cccgacttta ataggctttc
     2761 gacgcgattc cctagcgtat ctgccagcgt cagatactct ggagataatg acttttgggt
     2821 atttcgagga agagtagcta atcgctttgg ttatgaacaa tatggtaaac atagtgagga
     2881 tattcttgct catcgagaat attcagggcc tacattctgt gcgggagata gagacataga
     2941 atattatgca aacgagtatc aagcctacaa agccaatccc tctggtaact ataaatttgg
     3001 tagcccagag gtatggcgta ggattgggca aaaccatcac ataactaagg ttgttgagca
     3061 actcgccact ctttacgggc tttaagttta actcgaacag cttcacgaag ctcgccaaca
     3121 tccatactat tggcgagttt tgaccataag acgcgtttag gtttattttt caaattctgt
     3181 agctccccta catcacttaa tagggaaagc aattcatctt tccaaagaag cattgttaag
     3241 gaaagtttgt ccacttgtgg attcaacttg ctagttcgaa ttgtttttaa atggatacct
     3301 tgccgtgcac cttgcgtaac ttgctttatc ccccaccatg atggaactat gcttaaagca
     3361 tcataaagat ggcaatcaga gacaaccaaa gttactttat ccatcactga tgagtaatgt
     3421 tgcacctgcg ctggtaatct gagcaagtta tcactcttgc tcttcaactc ataaccatgt
     3481 ataagcccat tgatcactgc tatatcagct ctgctagccc ctagattcat cgtaaactca
     3541 tcaatgatta gggtgtcagg atctttatga tgatctctca gaatcttggc atgcacagcc
     3601 tttcttacat caatgtctct cataccatag ttgcgctttc atcgactctc tcaagccatg
     3661 attctatatc atggttcgca tgaatgccat ttgatatgac cagtggttaa acattgcaca
     3721 gaaaagtgga tgtgtatttt aacaaaagca atgacggcta accatgttgg tatgtcgata
     3781 aaatgtttca acgtaagcat tatctgttcc gccctttcag acggcctcct gatgttctga
     3841 gggtgcagaa tccctccggt taagggttta ataaaaatcg tttctgattt aaatcttcag
     3901 tatttagttg ttagtcggtt tatagccttt atgcttcggc cttatttctc agccatacac
     3961 aaaccgggcc atcttcggtg tcatgtattg aaccaataaa ccatccattg ccctctggtc
     4021 gttccggttc ccatgcagaa atatcagcat cacacgcatc aaggtcagca catccttcat
     4081 ctctgaagca gaggacgtat tgaagattat tttcctccat ccaggcgtta aactcttccg
     4141 ttgaaatata ttcccgaccg tcacagaatt tttcatattc aggatgcgtc cagcagccat
     4201 attcatcacg tactactggt atttctttaa tttcattcat ttctgttctc ccacgttttc
     4261 agactttcac cacagaacgg acaaaatgaa acccgaactg gtaatttaga aaattcaccg
     4321 gaacgcaaca tcacaaaatc aggaccgcga gttaaactct cattccagat tttgtatatc
     4381 agcagacctt ttcgcatcgt gtattcagca tcatgctcaa gggactttgc cagtgctgca
     4441 catggttcta tcttgttgcc attaacctgg cattttgatt cactcaccgc accacctcct
     4501 caaaattccc ctgataaaac gccagtacgc gctgcataac ttcgctcttc cggcactcga
     4561 gacagattat gttcagacgc ctgtcgtagc ggcgtatttc gccatctggt aatgaccaga
     4621 taaggtccgg atcaaccgca gatggtttct tcggctttgc ccttgagagc tttttacggg
     4681 cattttgcca gtccttacgc gcctgttcag acgggaataa cccgtaacca gagttgtata
     4741 catcgccact ggcaaccagc tctctggcca gaacgctcat cagatatctt gttgccccag
     4801 ttttagtttc cagttgtcgt aacgtctcgc gcccactctg gcgtacgagt tcaacaacct
     4861 gccctttaat tttttcccgc tcttcttgtg taaaaacttt tgccacaagc cctcctgaaa
     4921 attacctcat gaccagaaat taacacttac cccctgaagc ccggcggaat ttcagtgtcc
     4981 ggttcagaaa tgtgattcac gcaacgctgc gcaggcgaac gccccaggcg gataaccagt
     5041 tcatcccatt tttcccggag ttttgccgga ctcatgatgt tttttaccca gaacgaatcc
     5101 cgctggagac gcccaaacat ttcacaaatt tgtctgtgag ttctgccatc cagcatccgc
     5161 attgtgcgaa cgtcattggc ccatgctgtc cagttgggtt ctttcggtct agtgatctcg
     5221 ccatcat
//
LOCUS       DAZFKM010000220         5123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFKM010000220 DAZFKM010000000
VERSION     DAZFKM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..834
                     /locus_tag="ACHKAY_004911"
     CDS             <1..834
                     /locus_tag="ACHKAY_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5703424.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
     gene            876..1271
                     /locus_tag="ACHKAY_004912"
     CDS             876..1271
                     /locus_tag="ACHKAY_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5703425.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            1283..1657
                     /locus_tag="ACHKAY_004913"
     CDS             1283..1657
                     /locus_tag="ACHKAY_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5703426.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            1648..2226
                     /locus_tag="ACHKAY_004914"
     CDS             1648..2226
                     /locus_tag="ACHKAY_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703427.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            2223..2618
                     /locus_tag="ACHKAY_004915"
     CDS             2223..2618
                     /locus_tag="ACHKAY_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5703428.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            2626..3366
                     /locus_tag="ACHKAY_004916"
     CDS             2626..3366
                     /locus_tag="ACHKAY_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703429.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            3382..3804
                     /locus_tag="ACHKAY_004917"
     CDS             3382..3804
                     /locus_tag="ACHKAY_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5703430.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            3786..4220
                     /locus_tag="ACHKAY_004918"
     CDS             3786..4220
                     /locus_tag="ACHKAY_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5703431.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            4213..>5123
                     /locus_tag="ACHKAY_004919"
     CDS             4213..>5123
                     /locus_tag="ACHKAY_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5703432.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT         1133 a         1313 c         1642 g         1035 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaagtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cacagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcctgcag aacatgcgag acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgcttaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga agtggatatg ggccgcagtg tggcgaacaa catcacgcag
      301 tccggcggta cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga tccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcgaag cggtgtccta taaggggatg
      541 tatggcgata cggcgatcgt cgtgtattcc ggacagtacg tggaaaacga cgtcaaaaag
      601 aacttccttc cggacaacac gatggtgctg gggaacactc aggcacgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggta ttaacgcctc tgcccgctac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga ccctgatgcg ttcgtgtccg tacaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agtccatgac gaaagatgaa ctgattgccc
      901 gtcttcaggt gctgggtgag caactgaacc gtgatgtcag cctgacgggg acgaaagaag
      961 aactggcact ccgtgtggca gagctggaag aggagcttga tgacacggat gacgctgccg
     1021 gtcaggacac atctgtcagc ccggaaaatg cgctgaccgg acatgaaaat gaggttgtat
     1081 cagcgcagcc ggataccgtg actgatacgg ctgatctggt cacggttgtg gcactggtga
     1141 cgctgcatac tgatgcactt cacgccacgc gggatgaggc tgtggcattt gtgctgccgg
     1201 gaacggcgtt ccgtgtctct gccggtgtgg cagctgaaat gacagagcgc ggcctggcca
     1261 gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg ctgccattgc
     1321 ctgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatga catccggtga
     1381 gcagtccggt gctgtgatac gtggtgtttt tgatgaccct gaaaatatca gctatgccgg
     1441 acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg atgatgtgcg
     1501 gcagctgcgg cgcggcgaca cgctgaccat cggtgaggaa aacttctgga tagaccggat
     1561 ttcgccggat gatggcggaa gctgtcatct ctggcttggg cggggcgtac cgcctgccgt
     1621 taaccgtcgc cgctggaagg gggatgtatg gccataaaag gtcttgagca ggccgttgaa
     1681 aacctcagcc gtatcagcag aacggcggtg cccggtgccg ccgcaatggc cattaaccgc
     1741 gttgcttcat ccgcgatatc gcagtcggtg tcacaggttg cccgtgagac aaaggtacgc
     1801 cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa tccgcaggcc
     1861 agaatcaagg ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc gcgggttgtc
     1921 ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg tggcggcagc
     1981 gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact gaaaaatggc
     2041 cggtggcatg ttatgcagcg tgtggccggg aaaaaccgtt accccattga tgtggtgaaa
     2101 atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg gatacggcgt
     2161 gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat ggtaataaag
     2221 cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag catgacaccg
     2281 gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt ccggcagttg
     2341 ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat acctggcagg
     2401 cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag ctggatgcgt
     2461 ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca gagttgatca
     2521 ccagtatggt ggccagtggc tatgactacc gacgcgacga tgatgcgggc ctgtggagtt
     2581 cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc tgtaccaaat
     2641 cctgtaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg gagcggtgac
     2701 ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt taaagacctg
     2761 acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga tgaagatgcg
     2821 gactggactg cgaccgggca ggggcagaaa tcagccggag ataccagctt cacgctggcg
     2881 tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga aggtgatacc
     2941 cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg ctgggtcagc
     3001 agtatcggta aagcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa ggtcaccaat
     3061 gtgggacgcc cgtcgatggc agaagatcgc agcacggtca cagcggcaac cggcatgacc
     3121 gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac cgtggcattc
     3181 cagccggaag gcgcaaccga caagagcttc cgtgcggtgt ctgcggataa aacaaaagcc
     3241 accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg caaggtcaac
     3301 attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac tgtcaccgac
     3361 agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca taacggcgtg
     3421 accgtcacgc tttctgaact ttcagccctg cagcgtattg agcatctcgc cctgatgaaa
     3481 cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttacagtgga agacgccatc
     3541 agaaccggtg cttttgtggt ggcgatgtcc ctgtggcata accatccgca gaagacaaag
     3601 cagccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac cacctggccc
     3661 acagaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat gtatgagttt
     3721 gtggtgaatg atgcccctga acaggcagag gacgccgggc ctgcagagcc tgtttctgcg
     3781 ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgcgag atggggcgac
     3841 ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac tggcaccgct
     3901 tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc gggctgacgt
     3961 acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat ttcagtctgc
     4021 tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag aaagcggcag
     4081 ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc gcttccccgg
     4141 atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa gggatcgcag
     4201 gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag tctggatgcg
     4261 gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac ggaaagtgat
     4321 gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct ggctgcacag
     4381 aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc tgcacagttc
     4441 accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct gctgcaacag
     4501 ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg gcttgccggt
     4561 gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg tgcgctggcg
     4621 tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct ggtcctttcc
     4681 ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc cgggcaggcg
     4741 gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa ggcgggggta
     4801 agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc ctctgcatcc
     4861 ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga cccgacgtcg
     4921 gggctgacgg cgatggcacg ccagttccat aacgtgacgg cggagcagat tgcgtatgtt
     4981 gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa cgaggccgca
     5041 acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac gctggagacc
     5101 tgggcagaca ggactgcgcg ggc
//
LOCUS       DAZFKM010000221         5104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFKM010000221 DAZFKM010000000
VERSION     DAZFKM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..596)
                     /locus_tag="ACHKAY_004920"
     CDS             complement(<1..596)
                     /locus_tag="ACHKAY_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO5703433.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHKAY_004921"
     tRNA            complement(711..786)
                     /locus_tag="ACHKAY_004921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHKAY_004922"
     tRNA            complement(793..867)
                     /locus_tag="ACHKAY_004922"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(983..1067)
                     /locus_tag="ACHKAY_004923"
     tRNA            complement(983..1067)
                     /locus_tag="ACHKAY_004923"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Tyr,seq:gta)
     gene            complement(1076..1151)
                     /locus_tag="ACHKAY_004924"
     tRNA            complement(1076..1151)
                     /locus_tag="ACHKAY_004924"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1116..1118),aa:Thr,seq:tgt)
     gene            1513..2463
                     /gene="coaA"
                     /locus_tag="ACHKAY_004925"
     CDS             1513..2463
                     /gene="coaA"
                     /locus_tag="ACHKAY_004925"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO5703434.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2492..3457)
                     /gene="birA"
                     /locus_tag="ACHKAY_004926"
     CDS             complement(2492..3457)
                     /gene="birA"
                     /locus_tag="ACHKAY_004926"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO5703435.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3454..4482)
                     /gene="murB"
                     /locus_tag="ACHKAY_004927"
     CDS             complement(3454..4482)
                     /gene="murB"
                     /locus_tag="ACHKAY_004927"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO5703436.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4785..4900)
                     /gene="rrf"
                     /locus_tag="ACHKAY_004928"
     rRNA            complement(4785..4900)
                     /gene="rrf"
                     /locus_tag="ACHKAY_004928"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4995..>5104)
                     /locus_tag="ACHKAY_004929"
     rRNA            complement(4995..>5104)
                     /locus_tag="ACHKAY_004929"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1278 a         1316 c         1158 g         1352 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtgccgaa attggtggtg ggggaaggat tcgaaccttc gaagtctgtg
     1021 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccgga cttgatggtg
     1081 ccgactaccg gaatcgaact ggtgacctac tgattacaag tcagttgctc tacctactga
     1141 gctaagtcgg catcaagtag cgcgcattct atggagacat gcgagttcat gcaactaaaa
     1201 aattgcataa tttgttttat tggtcacatt ttatgcgaca cgatgaagaa acagccgaac
     1261 ggagggcaga ttagcacact ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa
     1321 ccataattct ttttatcaga tggaatatct gtcacattgc ttttcaacga tagcttcctg
     1381 gcagagattt tttcttatta ttcctcccca tctggtgtta ccctcctgcc cattaaccca
     1441 ttcaacagaa ctgtgacgcg ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc
     1501 agaaacatgc ttatgagtat aaaagagcaa acgttaatga cgccttacct acagtttgac
     1561 cgcaaccagt gggcagctct gcgtgattcc gtacctatga cgttatcgga agatgagatc
     1621 gcccgtctca aaggtattaa tgaagatctc tcgttagaag aagttgccga gatctattta
     1681 cctttgtcac gtttgctgaa cttctatata agctcgaatc tgcgccgtca ggcagttctg
     1741 gaacagtttc ttggtactaa cgggcaacgc attccttaca ttatcagtat tgctggcagt
     1801 gtcgcggtgg ggaaaagcac aaccgcccgt gtattgcagg cgctattaag ccgttggccg
     1861 gaacatcgtc gtgttgaact gatcactaca gatggcttcc ttcaccctaa tcaggttctg
     1921 aaagaacgtg gtctgatgaa gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg
     1981 gtgaagtttg tttccgatct caaatccggc gtgccaaacg ttacagcccc tgtttactcg
     2041 catcttattt atgatgtgat cccggatgga gataaaacgg ttgttcagcc tgatatttta
     2101 attcttgaag ggttaaatgt cttacagagc gggatggatt atccacacga tccacatcat
     2161 gtatttgttt ctgattttgt cgatttttcg atatatgttg atgcaccgga agacttactt
     2221 cagacgtggt atatcaaccg ttttctgaaa ttccgcgaag gggcttttac cgacccggat
     2281 tcctattttc ataactacgc gaaattaact aaagaagaag cgattaagac tgccatgaca
     2341 ttgtggaaag agatcaactg gctgaactta aagcaaaata ttctacctac tcgtgagcgc
     2401 gccagtttaa tcctgacgaa aagtgctaat catgcggtag aagaggtcag actacgcaaa
     2461 taatttgcag gggagcgaat actccccttt cttatttttc tgcactacgc agggatattt
     2521 caccgcccat ccagggtttt attattccat cctgctcaag taataaagcc ccctgtttat
     2581 ctattccgcg tgaaatgcca aatatttctt tatcaccaat gataagtttc actgggcgat
     2641 taataaaatt atccagcttt tcccagcgcg acagataagg tgccaatcct tcttgttcga
     2701 agagttccaa cgcagcacgt aattcacgta ttagcatggc cgccaacgta ttacgatcga
     2761 gattgatccc cgcttcctgc agcgtgatcc acccctgatt aacgacactc tcttcaacac
     2821 ggcgcattgc catgttgatc ccggctccaa tgactatttg cgccgcatcg ccagttttgc
     2881 cagtcagctc cacaagaatg cctgccagct tgcgatcctg cagatagagg tcattaggcc
     2941 atttgacacg aactttatct gctcccagct tgcgtaatac ttccgccatc acgataccga
     3001 taaccagact taaaccaatc gccgccgccg ggccttgttc cagacgccag aacatcgaca
     3061 aatataagtt tgcgccaaaa ggcgaaaacc atttccgccc ccggcgacca cggccagcct
     3121 gctggtattc tgcaacacag gcatcgcccg atttaagctc tccgatacga tcaagaaggt
     3181 actgattcgt ggagtcaata actggcagca cggctacact accgccatcc agctgaccca
     3241 atatctgttc agcattaagt aactggatgg gctcaggcag gctgtatcct ttacccggaa
     3301 cggtaaagac atcaacgccc cagtcacgca gtgtctgaat gtgtttatta atagccgccc
     3361 ggctcattcc cagcgtttca cccaactgct cgccagagtg aaattcaccg ttcgctaaca
     3421 gggcaatcaa tttcagtggc acggtgttat ccttcatgaa attgtctcca ctgcgctcac
     3481 ttcacctgat gcaccaataa agcggacttc aggctcaagc cagacattaa atttttcacc
     3541 cactttttgt cttacatggt gtgccagttg caccacatct tcgctttttg cattgtcttc
     3601 attaatgaga actaacgcct gttgacggtg caccgcagcc ccacccattt gcatcccttt
     3661 tagctggcac tgatcaataa gccaacctgc tgccagtttt actgaaccac ccgcctgggg
     3721 gtaatttggt gctgttggaa attgcgccag taatgcttta gccgtttcgg cagatacaac
     3781 agggtttttg aagaaactac cggcattgcc attcactttt ggatcaggga gtttggtggt
     3841 gcgcatatga cacaccgcat taaatacttg ctgtggcgtt actgttgtag gatccagacg
     3901 agttaagtca ccatacgtta gtacaggttg ccactctttt ggcagacgca gacctacggc
     3961 tacaatggcg aagcgatcct ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg
     4021 gcactctttg gcagttaagc gcacttgctt gcctgtcgcc agttcaacac aatcaacata
     4081 agcgcaaact cgctgtaatt ctacgccata agcaccaata ttctggatag gtgatgagcc
     4141 gacacaacca ggaattaatg ccagattttc cagaccaggc ataccttcct gcaaagtgta
     4201 ttttaccagg cgatgccagt tttctccggc tcctacatgt aaataccacg catcaggttc
     4261 atcatgaatt tcgatacctt tgatccggtt gatgatcacc gtgccgcgat agtcttccag
     4321 aaaaagtaca ttacttcctt cacccagaat aagaacgggt tgtccttctg cggttgcata
     4381 ctgccaggca ttgagtaatt gttgttcgtc ttcggcacat acaatgtgct gagcattatg
     4441 atcaatgcca aatgtgttcc agggttttaa ggagtggttc atagctgctt tcctgatgca
     4501 aaaacgaggc tagtttaccg tatctgtggg gggatggctt gtagatatga cgacaggaag
     4561 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct
     4621 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa
     4681 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4741 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     4801 ctctcgcatg gggaaacccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     4861 gcatggggtc aggtgggacc accgcgctaa ggccgccagg caaattctgt tttatcagac
     4921 cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaaaac
     4981 atcttcggcg ttgtaaggtt aagcctcacg gttcattagt accggttagc tcaacgcatc
     5041 gctgcgctta cacacccggc ctatcaacgt cgtcgtcttc aacgttcctt caggaccctt
     5101 aaag
//
LOCUS       DAZFKM010000222         5073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFKM010000222 DAZFKM010000000
VERSION     DAZFKM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..957
                     /gene="ybeF"
                     /locus_tag="ACHKAY_004930"
     CDS             4..957
                     /gene="ybeF"
                     /locus_tag="ACHKAY_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO5703437.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1166..2131
                     /gene="lipA"
                     /locus_tag="ACHKAY_004931"
     CDS             1166..2131
                     /gene="lipA"
                     /locus_tag="ACHKAY_004931"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO5703438.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2233..2436)
                     /gene="tatE"
                     /locus_tag="ACHKAY_004932"
     CDS             complement(2233..2436)
                     /gene="tatE"
                     /locus_tag="ACHKAY_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO5703439.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2565..3353)
                     /locus_tag="ACHKAY_004933"
     CDS             complement(2565..3353)
                     /locus_tag="ACHKAY_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO5703440.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3446..3829
                     /gene="crcB"
                     /locus_tag="ACHKAY_004934"
     CDS             3446..3829
                     /gene="crcB"
                     /locus_tag="ACHKAY_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO5703441.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3884..4093)
                     /gene="cspE"
                     /locus_tag="ACHKAY_004935"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3884..4093)
                     /gene="cspE"
                     /locus_tag="ACHKAY_004935"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5703442.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4268..4828)
                     /gene="pagP"
                     /locus_tag="ACHKAY_004936"
     CDS             complement(4268..4828)
                     /gene="pagP"
                     /locus_tag="ACHKAY_004936"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO5703443.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1371 a         1265 c         1124 g         1313 t
ORIGIN      
        1 tgcgtggata gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa
       61 ccacaaatat ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct
      121 gtatatgtac ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca
      181 atcagtcagt ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa
      241 ggccagggtg tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc
      301 cttgagtcca ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata
      361 actattgcga ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt
      421 aaaactcact atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa
      481 ctcagtcagt ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg
      541 caacatcatg ttctgttcac cgacaatatg gtgttaattt gccgcgaggg aaatccacta
      601 ctctctttag aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg
      661 gaagagcaaa atttcagcgg tctgcggcag agagttcaag aaatgtttcc ggaccggcaa
      721 atcaatttca ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg
      781 ttagcgatta ttcccagccg tttttataac ctgtttagcc gctgctggcc gctggaaaaa
      841 ctgccttttc cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc
      901 agcctgcgtg atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa
      961 agtattgtta cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt
     1021 ttacattttc gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc
     1081 gttaattcaa aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt
     1141 aattcgcaac tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa
     1201 ataccgcgat gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga
     1261 agccctgctg cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat
     1321 ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc
     1381 ctgccctaac ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc
     1441 tatttgtacc cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga
     1501 tgccaatgaa ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt
     1561 tatcacctcc gttgaccgtg atgacctgcg cgatggcggt gcccagcact ttgcggattg
     1621 cattactgcc attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt
     1681 ccgtggtcgt atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa
     1741 ccataacctg gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa
     1801 ctggtcgctg aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc
     1861 tggtctgatg gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct
     1921 gcgccgtcat ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca
     1981 cctgccggtt caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct
     2041 ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga
     2101 tttgcaggcg aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga
     2161 taaaaaaacc cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc
     2221 ctgctcgcca cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc
     2281 ttttttcgcc gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc
     2341 aaggtctccg cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc
     2401 aactactagc agtttggtaa tactaatctc acccatagat accttcttga catataaccg
     2461 gcaacagaac gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa
     2521 tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc
     2581 ggcgcaaagc gacggttgtt taagacgggc agttgcgcgc gcacctgacg cacacgttcg
     2641 ggcgtcactt ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca
     2701 aagggatcta taatccggct ttgaccgata tttttgttcc cgcactcccc cgccgccacc
     2761 atataacagg tggtatccag cgcacgagcg gcaagcaacg ttgaccaatg atgctctttg
     2821 agcggcccgc gaacccaggc ggcaggaagt accaggattt cagctccctg taatgcctgt
     2881 gccagcgcca gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatcccc
     2941 tccacctcga gtaacggagc gatttcatta ccagcatcaa cacggcgtga ttcctgaatg
     3001 gcaaatgcat catagagatg caatttggca taacgggcga cgatgtttcc tgcctgtagc
     3061 gccaccagca tattccatgc gcgccccggc gttgaaggaa catgaatcgt cagaattgtc
     3121 gtcatcatgt tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc
     3181 tgtgctgatt taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc
     3241 aggacgaaca gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca
     3301 gcgttctttt cccacacaga tgtaacagca aactgtcctg cggcaaccag cattgccacc
     3361 tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat
     3421 acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg
     3481 tggtacggga agcgtggcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc
     3541 gattccgttg gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt
     3601 cgcctggttc agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg
     3661 attttgtggc ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca
     3721 agagggccgc tttggctggg cgttactgaa cgttttcgtc aaccttctgg ggtcttttgc
     3781 catgacagca ctggctttct ggctgttttc ggcctcaacc gcacactaaa gaccaaaaaa
     3841 aaacccgctg attaagcggg ttttgaattc ttgctgacgt atcttacaga gcgattacgt
     3901 ttgcagcaga agggcctttg gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag
     3961 caagagtttt aaaaccatta gtctggattg cagagaagtg tacgaacacg tctttgctgc
     4021 cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt aaaccactta acgttacctt
     4081 taatcttaga catcaaaatt acctttacat gaaaaataga cacaaatgct gtgtcggtta
     4141 ccagtacacc aattgtggta cgctttgtcc agtgcaactt tactaaaaag tgataaaagt
     4201 cgcgttattt ttgatgttac ttattttagc tattgatttt aaagaagtta ctaaaacttc
     4261 atttgtctca aaactgaaag cgcatccagg caaagtacac attgccattg ttgtaggtac
     4321 ccggaatgta ggtcatctga aaagtcactg ggccataacc cacggaggcc aatggcagta
     4381 gaaccgggag agggatgtaa ttccagttat cgcgtgccgt tacgccagcg gtgaatccca
     4441 gacctaaatg aaaattttca tccgccagcg gtcgccaggt actttcccat ccgtatccgg
     4501 caatcggttc ccatttgttc cacgagtcct taaatgccat ggcatacagg ccatgccagt
     4561 ttcctttttc atcccaacgc gacaggccaa aaccgccacc ccacggtcgc tcgttatagc
     4621 gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca ggtgatggca ggaatatata
     4681 aatcataatg ttcaggctgt tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc
     4741 actcatctgc gttagcaaaa actttaccaa cgctgattaa ctgaataaaa acaaaggaaa
     4801 agatagcgac atatttactc acgttcattt gtgaccataa aacatttatc aaaaatctac
     4861 tactagcata gcaaagctac aattaacata accttaatag acccaacata aagaataatc
     4921 tgaatagctg ctctatttat ttccgcttgt tcaagttaaa tatttccgcg catcgattaa
     4981 agatgatgcg tcgttttctg cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg
     5041 atgacaaaat gcaatcaagg aaaaattatt tta
//
LOCUS       DAZFKM010000223         4987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFKM010000223 DAZFKM010000000
VERSION     DAZFKM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..165
                     /locus_tag="ACHKAY_004937"
     CDS             <1..165
                     /locus_tag="ACHKAY_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFO5703444.1"
                     /translation="GGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSEL
                     LPWRIALPAE"
     gene            608..4510
                     /gene="espP"
                     /locus_tag="ACHKAY_004938"
     CDS             608..4510
                     /gene="espP"
                     /locus_tag="ACHKAY_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFO5703445.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRATGKKKHKRILALCF
                     LGLLQSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKYSYNVDMSGAQVATIENGKLTG
                     TGSDTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSTVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SILSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSVGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEN
                     GADVTTQSGMFSTSDISISGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTNL
                     SESDKLEVKKHLSGENNILLVDFLQKPTPEKQLNIELVSAPKDTNENVFKASKQTIGF
                     SDVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTD
                     SSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4758..>4987)
                     /locus_tag="ACHKAY_004939"
     CDS             complement(4758..>4987)
                     /locus_tag="ACHKAY_004939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703446.1"
                     /translation="AQNQPAETVRAAAAATGRFAVAFPVRVAPGSGCFMKTNAPRLFF
                     VDKEAGVSFDVFHVLSVSFPVTACATHDGGM"
BASE COUNT         1513 a         1132 c         1113 g         1229 t
ORIGIN      
        1 ggtggtgagc ggggagcgct actgtacagc ctgatcggga cgtgcaaact gaatgacgtg
       61 gatccagaaa gctaccttcg ccatgtgcct ggcgtcatag cagactggcc ggtcaaccgg
      121 gtcagcgaac tgcttccgtg gcgcatagca ctgccagctg aataacacat ccccgtcaat
      181 acggccctcg ctgtacgctt acgcataaat attgaataca tttactgagc atgcaaaaga
      241 aaaagacaaa ctaatcccgg cctttcacag aatttcctct atcagaggct gagttgacaa
      301 cctgctcacc caggaaatag taacacccga aaacatgaag agactctgat aaacaatctc
      361 gatacatatt cctcatcttt cacagcatct cacttcttta acaggaatta ttattatcta
      421 cgcctcctcc tttcatgtat ccctcatgaa tgattaagat aaacatactg tgtttgttat
      481 ccgtatgata acaaacacat aaaggagtaa acatttatct ggatgatgat tacaatgttc
      541 acgactgtca gagacacacc tctgatacat tagaaaaacc aatgtttccc ttaaaaatgg
      601 agcttatatg aataaaatat actctcttaa atacagccat attacaggag ggttaatcgc
      661 tgtttctgaa ttatccggca gagtatcatc aagagcaact ggtaagaaaa aacacaaacg
      721 catacttgca ttatgttttt taggcttatt acaatcctca tattcttttg cgtcacagat
      781 ggatatttca aatttctaca tccgtgacta tatggatttt gcacagaaca agggcatatt
      841 tcaggctggc gcaacaaata ttgaaatagt gaagaaagat ggctccaccc tgaaactacc
      901 ggaagtacca tttcctgact tctcaccggt tgcaaacaaa gggtcaacca catctattgg
      961 tggtgcatac agtatcacag ccacacacaa tacgaaaaac caccactcag ttgcgacgca
     1021 aaactggggg aacagcacgt acaaacaaac tgactggaat acttcacatc ctgattttgc
     1081 agtatcccga cttgacaagt ttgttgttga gacccgaggt gcgactgaag gcgcagatat
     1141 ttcgttatca aaacagcagg cacttgaacg ttacggggtt aattataaag gagaaaagaa
     1201 acttatcgca ttcagagccg gctctggtgt ggtatccgtt aaaaaaaatg gacgcataac
     1261 tccatttaat gaggtttctt ataagccaga aatgttaaat ggctctttcg ttcacattga
     1321 tgactggagt ggatggctga tattaaccaa caaccagttt gatgagttta ataacattgc
     1381 ctctcagggt gacagcggtt cagcactgtt cgtctatgat aaccaaaaga aaaagtgggt
     1441 tgtcgctgga actgtctggg ggatttataa ttacgccaat ggcaaaaacc acgcagcata
     1501 cagtaaatgg aaccagacaa ccattgacaa cctgaagaac aagtattctt acaacgtgga
     1561 tatgtcagga gctcaggttg caaccattga aaatggaaaa ctgacaggca ctggctcaga
     1621 caccaccgat ataaaaaata aggacttaat atttactggc ggtggagata tcctcctgaa
     1681 atcctctttt gataatggtg ctggcggtct tgtctttaat gataaaaaga cctatcgagt
     1741 aaacggggat gatttcacct ttaaaggtgc cggtgttgat acaagaaacg gcagcaccgt
     1801 tgagtggaat atccggtatg ataataaaga caaccttcac aaaattggtg atggcacatt
     1861 agatgtccga aaaacccaga acaccaacct gaaaacaggt gagggtcttg tcattcttgg
     1921 agctgaaaaa acattcaata atatctacat aaccagtggt gatggaactg tccgactgaa
     1981 tgcagaaaat gcactgtctg gcggtgaata caacggtatt ttctttgcga aaaatggcgg
     2041 aactcttgac ctgaacggat ataatcagtc tttcaataaa attgctgcaa ctgattcagg
     2101 tgctgtaata accaatacgt caaccaaaaa atccatttta tccctgaata atactgctga
     2161 ctatatctat cacggcaaca taaacgggaa tctggacgta cttcagcatc atgagacgaa
     2221 aaaagagaac cgtcgtctta ttcttgatgg gggcgtggac acaacaaatg atataagcct
     2281 gcgtaataca caactgtcca tgcagggaca tgccactgaa catgccattt atcgggatgg
     2341 agctttctct tgttcactac cagctcctat gcgctttttg tgtggcagtg attatgttgc
     2401 aggaatgcaa aatacagaag ctgatgctgt aaaacaaaac ggaaatgcct ataaaaccaa
     2461 caatgctgtc tctgatttat cgcagccaga ctgggaaacc ggaacattca gatttggaac
     2521 gctacatctt gaaaattccg atttttctgt tggtcgtaat gcaaatgtaa tcggggacat
     2581 tcaggccagt aaatcaaaca ttactattgg tgacactaca gcatatattg atttgcatgc
     2641 tggtaaaaat attaccggtg atggttttgg cttccgccag aatattgtgc gtggaaactc
     2701 acaaggagaa acgctgttta caggagggat cacagcagaa gacagcacta tcgttattaa
     2761 agataaagca aaagcattat tttcaaatta tgtatacctg ctgaacacaa aagcaaccat
     2821 agagaacggt gctgatgtga caactcaaag tggtatgttc tccacgagcg atatcagcat
     2881 ctctggtaat ctgtccatga caggcaatcc cgacaaagac aataaattcg agccctcaat
     2941 atatctgaat gatgcttctt atctactgac tgacgactcc gccagactcg ttgccaaaaa
     3001 taaagcatct gtggtgggag atatacactc cactaaaagt gcatccatca tgtttggtca
     3061 tgatgaaagc gacctctcgc agttgtctga cagaacctca aaagggcttg cacttggtct
     3121 tttaggtggc tttgatgtct catatcgcgg ttcagtcaat gccccgtcag catctgccac
     3181 tatgaacaat acctggtggc aactaaccgg agattctgcg ctgaaaacac tgaaaagtac
     3241 aaacagcatg gtctatttca ctgacagcgc aaacaataag aaattccata cgctgacggt
     3301 cgatgagctg gcaaccagca acagcgccta tgcgatgcgt acaaaccttt ctgaatcaga
     3361 caaactggag gtcaaaaaac acctgtctgg tgagaacaat attttactcg ttgatttcct
     3421 tcagaaacca acgcctgaaa aacaactgaa tattgaactg gtaagcgcgc caaaagacac
     3481 caatgaaaat gtctttaaag ccagtaaaca aaccattggt ttcagtgatg taacgccggt
     3541 cattacaacc agggaaaccg atgacaaaat aacatggtca ctgacaggct ataacacggt
     3601 agcaaacaag gaagcaaccc ggaatgccgc cgccctgttc tctgttgact ataaagcgtt
     3661 tctgaacgag gtcaacaacc tgaacaaacg tatgggtgac ctgcgtgata tcaacggcga
     3721 agccggtgca tgggcacgca tcatgagcgg taccggctct gccagtggtg gtttcagtga
     3781 caactacacg cacgttcagg tcggggtcga caaaaaacac gagctggacg gactggattt
     3841 gtttaccggt ttcactgtca cacacactga cagcagtgcc tccgccgatg ttttcagtgg
     3901 taaaacgaag tctgtggggg ctggcctgta tgcttccgcc atgtttgatt ccggtgccta
     3961 tatcgacctg attggcaagt atgttcacca tgataatgag tacactgcaa cctttgccgg
     4021 actcggaacc cgtgattaca gcacgcattc atggtatgcc ggtgcagaag cgggctaccg
     4081 ctatcatgtc actgaggatg cctggattga gccacaggct gagctggttt acggttctgt
     4141 atccggtaaa cagtttgcat ggaaggacca gggaatgcat ctgtccatga aggacaagga
     4201 ctacaatccg ctgattggcc gaacgggtgt ggatgtgggt aaatccttct ctggtaagga
     4261 ctggaaagtg acagcccgtg ccggtctggg ctaccagttc gacctgctgg ctaacggcga
     4321 aaccgtattg cgggatgcat ctggtgaaaa acgcatcaaa ggtgaaaagg acagccgtat
     4381 gctgatgtcc gttggcctga atgcagaaat cagggataac gtccgctttg gactggagtt
     4441 tgagaaatcc gcctttggta agtacaacgt tgataatgca gtcaacgcta atttccgtta
     4501 ctcgttctga tactccgtat tcaaaagggc ctgcgggccc tttacgctca gtgcctgccg
     4561 ccctgatatg tctgagagac atttccggga tgtcagtcga ctggtttact taactgcgat
     4621 gactaaagct ggcagtaatg ccggcttttt tacggccaca tacgtcttcc tctcatccgg
     4681 aactgtgata caaatcctgc atacaggatt gaccgccggt tcctgcaaat ggcacatttt
     4741 cagtaagata gcgaaggtta cattcccccg tcatgtgtgg cacaagctgt caccgggaaa
     4801 gaaacggaaa gcacatgaaa aacatcaaaa ctcacaccgg cctccttatc cacaaagaac
     4861 aaacgcggcg cgttcgtctt catgaaacac ccactgcctg gtgccacacg caccgggaat
     4921 gctacagcaa aacgaccggt cgccgctgcg gcagccctga cagtctcagc aggctgattc
     4981 tgagcag
//
LOCUS       DAZFKM010000224         4939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFKM010000224 DAZFKM010000000
VERSION     DAZFKM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..358)
                     /locus_tag="ACHKAY_004940"
     CDS             complement(<1..358)
                     /locus_tag="ACHKAY_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5703447.1"
                     /translation="MTLTLLTAYNVPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLI"
     gene            complement(625..1290)
                     /locus_tag="ACHKAY_004941"
     CDS             complement(625..1290)
                     /locus_tag="ACHKAY_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO5703448.1"
                     /translation="MTILSLENPVILLLENMLEALSTAPDSINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(1431..2072)
                     /locus_tag="ACHKAY_004942"
     CDS             complement(1431..2072)
                     /locus_tag="ACHKAY_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO5703449.1"
                     /translation="MCLSELQAGLNEDQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNIRELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAPPLPESRIALLLHLVSDDGALT"
     gene            2774..3805
                     /gene="repA"
                     /locus_tag="ACHKAY_004943"
     CDS             2774..3805
                     /gene="repA"
                     /locus_tag="ACHKAY_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFO5703450.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATSFLVEPERK
                     RTRRRRGEHSTKPKCENPSWYRPARYKELSGQLGHAYNRLVKKAPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAKEISPKD
                     SKGNVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT         1328 a         1208 c         1303 g         1100 t
ORIGIN      
        1 caatcagtct gccggtgcgg tgtacgagag gacatgtcag cagcaggcaa agtggtgcaa
       61 ggagaatgtt cggggcagga gcctgcagca gatgaataat aagaccctgc aggagaatgc
      121 cggatatacc aaaaaaacct gccagcatac agaggacgat cagcaggggg atccgtccga
      181 tgttcagcca gtccagcgcc tctcctgtca gcccctccgg taaatcaggt gtatccatag
      241 ctgaagagag ggagtgtccg cagagcaggg cgataagttc agcgatacca gtaagcagaa
      301 caaaagtcag ggctgccaga tagggtacgt tatacgcggt aagtaacgtc agtgtcattt
      361 ttcctcctgt ggcaggtcag tcaaacaaca ttatggcgtg aagcttccgc ttctttaatt
      421 gtcagaagaa gcatatcgtc agtcatcaga agacaagggg atgtctgcag gaggggatga
      481 taacaactga agcctcacgc cggaatgcgc tttatgacaa ccggagagga cggataataa
      541 aaaatgggac atccacatca gcaaaccgcg atttgcgaaa aaaagtcagc ggagagtggc
      601 tgccgttgtg gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca
      661 ttcggctttc ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc
      721 gctgccgttc aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat
      781 ctcttccact tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct
      841 gagcattctg cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg
      901 tttacctgtt tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt
      961 gccaatctgc catcccagac ttctgcacgc ggaaagagcc gagcggaagc ggtacaggtc
     1021 gtataatgct gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat
     1081 gtccgtattg gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa
     1141 tgcgctcatc tgacgcgcag attcaagcca gttcagcagg taacgccggc gacgccgttc
     1201 gttgttgata ctgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat
     1261 gacagggttt tccagggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc
     1321 gctctccgta caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aaggaaaaaa
     1381 aatcccggcc ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac
     1441 gctccgtcat cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggcggt
     1501 gcatcaagcc ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca
     1561 tccagctcct ccctggctgc cggattgagc gcaggatccg ggtacacttt catcaaagac
     1621 tgaacgatat cctccctgac ggttttgatt tcaccggcca ttttcacgaa gttcagaaag
     1681 gcactgtgac ggcgaaggtg agtgaagctg tggcgttcgg ggtctgcttt caggaaaaag
     1741 taaccgggaa acacggcgtg tatatgtcga cggtaaccgc agactttatc cgtgcggcgg
     1801 acttttttca gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag
     1861 agatgttcgc ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt
     1921 aattctctga tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc agaggggctt
     1981 cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgatcctcg
     2041 ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg
     2101 acggtttcga ggccggcgaa aaccgacaaa acctgcaaac aggattaatg gctggcaaac
     2161 atattacata aggatatatc tgtggcaaga gcgaagataa gtagttaaat agatcgttat
     2221 atttgataaa gcagcatatt acgctgggtt gtgtgaggtg tttggatgat tagatcgtag
     2281 atcgtaatca caagatcgat tcatccaaaa aacatgaaaa acacagcata aaagatcgtt
     2341 tgtggaattg tctatttttt gacacagttc aaaaaaacga cgttttttag gtggttaatg
     2401 agaggcctgg taaatcagag aaatcgtgtt ggcttttcaa agcggtggaa aaggggtata
     2461 ttgcggatcg ttatgcagtg gcttttggga tcgtcaggat ccgggaagtc agaaaatggc
     2521 aggatgcgcc ataaggcatt caggacgtat ggcagaaacg acggcagttt gccggtgccg
     2581 gaaggctgaa aaaagtttca gaaggccata aaaggaaaac ccccataatc ttctcatcga
     2641 acttggcgga acgttagaag attatggggg cctcacagaa tacgggataa gtatatatga
     2701 aaccgtgtca gagattcaac ccttcgcagt gtattaacgc acggcattct gtttccgcca
     2761 taagcgacag cttgtggcag gtctgaagaa tactccatat aacgcagtac actggagtca
     2821 gttagcaccc gaagagcaga tccgtttctg ggaagactat gaagcgggaa gggcgacctc
     2881 tttcctggtt gaaccggaaa ggaagcgcac caggcgccgt cgtggcgaac actccactaa
     2941 acccaaatgc gaaaatccgt cctggtatcg tcctgcgcgc tataaggagc tgagcgggca
     3001 gctcgggcac gcctacaacc gtctggtgaa aaaggccccg gtgaccggcg agcagagcct
     3061 gcgtatgcgt atgtcgcggc atccttttta cgtgcagaaa cggacgttcg tcggtcgcaa
     3121 atatgctttc cggccggaaa aacaacgcct cctcgatgcc atctggccgg ttctggtcag
     3181 cttcagtgat gccggcacac acactgtggg gatgagtgtc actcgcctgg ctaaagaaat
     3241 cagtccgaaa gacagtaagg gaaatgttat tccggaactg gaagtgacgg tctcccgtct
     3301 ttcccgtctg ctggccgaac aggtgcgttt tggtgtgctg ggtgtttcag aggaaacgat
     3361 gtgggaccgt gaaacccgcc agcgtctgcc gcgctatgtc tggataacac cggctggctg
     3421 gcagatgctg ggcgtcgaca tggtaaaact tcacgaacag cagcagaaac gactgcgtga
     3481 aagtgaaatc cgccagcagc tcattcggga aggtgttctg cgtgaggatg aagatatctc
     3541 cgtacatgcg gccagaaaac gctggtatct gcagcgcagt caggatgcgc tgaaacaccg
     3601 tcgtgcgaaa gcagcagcca gtaagcgcgc cagacgcctg aagaaactgc ctgccgacca
     3661 gcagattcat gagatggcag agtatctcag gaagcgtctg ccgccggatg aagcctattt
     3721 ttgttccgat gaccatctga agcgactggc catcagggag ttgcgtcagc ttgaactgac
     3781 gctggctgcc ccgccaccgc actagacagc accattccct cagcactgaa tcatcgccag
     3841 cccctccggg gctttcggcg caggtatcgc tcagcccaaa atccgcagta atcaccttaa
     3901 atcccctcag aggggcatat ttgctcataa aaccacgcat cagtcatcag aacgtggcca
     3961 cgttgtttca gttatccaca taaatccgca aacaaagaac tttaagaagc tgcaaacctg
     4021 aaacagcaaa cctgcaatat agtcttaacc ccattattta atcccctgcg ttgcttcgcc
     4081 gcagcgaaag tctttatctc tgaaaccact gtgaacaaac acaaagaggc cttcgcttgc
     4141 agcggccaag gccgcgccgc tcagaatcta aaagcacctc ccacgctgac gcgcgggccc
     4201 cgaacctcac cgttctggaa ccacaacaaa aaaacatcag gaataaaaac accacacaaa
     4261 cgcagcaccg tgcccacccc tcataactga aaagcgccgc cgccccggcc caaagggccg
     4321 gaacagagtc gcttttaatt atgaatgttg taactaaaca tcatcatcgc tttaagaccc
     4381 catttgtcta gcagggcgat ggcccactgc gtgaaccatc accctgcaca cagggtattt
     4441 tatgtgcaat tgtcaaccac tgaatcggaa ccccagaggg gcccccacga ttcagaagtt
     4501 gacaatttaa gcacagggac tacatgagaa aaatcgtggt tcgtctgtca tacacgcgcg
     4561 taagcggcac tgatagccct cttacgcgga aatacgccca taagcgctaa cttaagggtt
     4621 gaaccgacgc cacggtgtct cgttaagact tcaattgcgt tttgtaggca gtcagggata
     4681 ctgtcccatg aatggccacc tgtaagctcc agataaccat ttttgttatt ctccacaacg
     4741 aattccgcat accggaaatc agcgctactt ctggggagga atggccttaa gagtgataaa
     4801 gattatatgc ttttcataga gagactcatc tatgccggta acactgaaaa atgtattgtg
     4861 ttccaagaag tttttttgag tgaacatatt ttcgctcaca acaatcgaat tattcaaagc
     4921 cacaatttta aaaaatatt
//
LOCUS       DAZFKM010000225         4935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFKM010000225 DAZFKM010000000
VERSION     DAZFKM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            141..877
                     /locus_tag="ACHKAY_004944"
                     /pseudo
     CDS             141..877
                     /locus_tag="ACHKAY_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1056..1352
                     /locus_tag="ACHKAY_004945"
     CDS             1056..1352
                     /locus_tag="ACHKAY_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703451.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1660..1776
                     /locus_tag="ACHKAY_004946"
     CDS             1660..1776
                     /locus_tag="ACHKAY_004946"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703452.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1995..3428
                     /locus_tag="ACHKAY_004947"
     CDS             1995..3428
                     /locus_tag="ACHKAY_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO5703453.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4249..4812
                     /locus_tag="ACHKAY_004948"
     CDS             4249..4812
                     /locus_tag="ACHKAY_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO5703454.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
BASE COUNT         1463 a          930 c         1113 g         1429 t
ORIGIN      
        1 ctgttaactg gttttcattt ttgaatcaat attttctgaa cagggtaata gtacacgtat
       61 taacagatta tgtcagaagg ccccggtgat gccttctgac gatagattaa tgttgtattc
      121 tattggtatc tggagaccat atggcaacag ttacagtata ttctccccgc tgtaattcct
      181 ataaagtgta ccgttatgat cacagcacct cagatcatga gcgtttccag tgctaatcct
      241 gcaaacgtgt atttcaactg aattacatct acgaaacccg aaaaccaggc attaaagagc
      301 aaattgttga aatggttcat aacggagccg gggttcgcga tagctccaga acgctgaagg
      361 tggatatcaa caccgtcatt ctcactttaa aaaacgctca ccacgtaaag taaccatatc
      421 tccagccgcc agtgtcgatg tggcacttat ctgtgaactt gatgaacaat ggagttttgt
      481 cgaaaacaaa gctcgtcagc agtggcactg gtacgcgtat aagaccaaag ctgacggtgt
      541 gctggcttac acttttggtc ctcgcactga tgaaacatgc cgtgagttgc cggaattcct
      601 gaagccattc tccgcaggca tgataaccag agacaaccgg agcagttata ccagagaaat
      661 gccacaggat aagcatcttg tcggcaagat atttacccgg cgcattgagc gcaacaatct
      721 gacacttcgt actcacatca aacgacctgc ccggaaaacg atttgctttt tacgctccct
      781 ggaaattcac gaaaagccat tggttcattt atcgaaaaca catgtactac tgacaggagt
      841 cattaccagg gcctcttttg ctgttttttt gccatgaatg ataatgagaa tcgacagcga
      901 taagggaaaa gtaaaataac actaatgctg tcgttttcta tcatattgat ttttaattat
      961 ttattgtctt ttttctcaac ccattttttt acgcagacaa atttacccgg ggaatacaaa
     1021 ctgtaacgac agttcaccac actatggggg ataaaatgcc agatcaatcc ggacactgca
     1081 ggactccttc acgcatctta cggatgcctg aactgagcca attgttaggt atatctcgtt
     1141 cgacaattta cgaaaaaatg aatccgttat cgaaatacta cgatgcaacc ttccccaggc
     1201 cggtacgtct tggtagtggt tctgtgggct ggcgttcatc agctattgac gaatggctta
     1261 ctttacatac tgtacctgcc agatcagcag tcaaaggtgt taatgacgaa gtgagacata
     1321 cgctttcttc agatatgacc atgagcagat aagtttttgt catcacgtga tcacctcagg
     1381 aggaaaaaga agcataagaa caatttagcc agaagtttaa ttgataaaca gaatttccag
     1441 cattcattgt tgaaagagca gctattgtcg ctattgtagc ggtaattgct gctataaccg
     1501 aaacgtatta cgaaaaagga agcatttatg ccttacatca aaccggatat gtcgtattca
     1561 gtatatgcat gaccatcaat gatagggaag gcaataatta aagctcaata caataaacaa
     1621 gtacctctga ctctggttgc aatgtcagta ttaacagcga tgtcaattgc ctgtcagaat
     1681 caggttgacg tatgcagtcc cggaaattta cgtggggccg ttaatattta cactatggtt
     1741 ctggagaaag ggaaaagacc gggggaaagg tattagtgaa ataatttccg gaggtattaa
     1801 gaatgagaaa tatgaatgat atttcgtggt gaataataca gtcttcactg aattaacctt
     1861 atcggtttat taaacatcag atctacaaat gctctgtatt tctcagagca tcaggtctga
     1921 atagcagcta ttttcgctat tgtagcggga tttgctgcta ttttactaac ccattaatga
     1981 aaagagaggc ttttatgtgc ttacttgcac cagaaaatcc ttatccgata tatgcactac
     2041 caccgctggt gagaaatgca ataattgaaa ctcaaaaaaa tacacaggca cctttggcta
     2101 tggtggcaac atccgcatta acagcgatct caattgcctg tcagaatcag attgacgtgt
     2161 gcagacctgg aaatttacat gggcctgtta atctttactc tctgattctg gctgattccg
     2221 gtgaaaggaa aacgaccgtg gataaagtgt ttatgaaagc attttatctc agggatgaag
     2281 ccctggcgga tgaatacgcg aaactggttg agaattacag tacagaaaag gaaatatggg
     2341 agcaaaaaca aaaagcgctg gaatcaaaat ttcataaaga gattcgtgcc ggtaaagatt
     2401 acaaggcaac agaatcagag cttgaaacgc atctgaataa acctcctgtt ccgccgcaga
     2461 tacgtcgaac aatttttaat gagacaacga tagagggaat gttaaaatat tactccgata
     2521 gcaatcgctc ttttgctctt gtatccagtg aagggggggt aatttttgac agccgggcca
     2581 tgagtaaact gggaattatt aacactttgt gggatggagg ttctcttttc atcgacagga
     2641 aatcatctcc cggaattaat ttgaaggagc caagactgac gatatcggtg atgattcagc
     2701 ctgatgttta ccacaaaggt ttttgtacgc gaaaaaaaga aattgtgaaa acgtcaggac
     2761 atcatgcaag gtttttgatg tgtcaaccaa catcaacgca ggggacaagg ataataaccg
     2821 gagataatta ttcatcacag tatcaggatt tgtttgaaga gaggatcaat gagcttattg
     2881 atgaaagtct ggcgatgagt ggtgaacgac gttgccttca cttttctcct caggctgcca
     2941 gaatctggac ggattattac aatgatgtgg aatcgaagct ggggggattg gggcctttaa
     3001 gacattgccg ggaatatgct gctaaaaatg cagagtatat ggcaagactg gcaggactta
     3061 tttaccattc gagcggtgaa gagggggaga tttcccctta cactgcagaa atggcgagag
     3121 aattagcaat atggtacggt aatgagtatg tgcggttgtc taatccgtta acttttgaca
     3181 actctgccct gaccgtacct gtgcgactca ttccagagga gcttgaactt ttcaactgga
     3241 taaaaagcta ttgtattgag aaggggatcc tctgtatgaa aaaaaatgac attttacagc
     3301 gtggtccgaa ccgtttccgg aaaaaggata aaatcaactg gttactggat ttactgtatg
     3361 aacaaaacag agttgtaccg gttattgagg gaaaaacgtt gtgtgtcgca cctaactttg
     3421 acctctgaac gacttaatcg ttgagtacat atagtctcct ctgctacttg cggaggagac
     3481 taatgtcttt accatgtcag cgttgtatta gaaaactctg gtacaggtca gtcagtaaat
     3541 ataactggtg taaccactat ttactattgg atagagtgtt actcaggata ttgtcggctt
     3601 tagtctgagg tgaggtgggg ccttcattgt tttcgtgttc aataaataat ttaggttaat
     3661 tagtattatt agtggaagtt atgtgatctg ttatgttgtc ttgtacgcac ttaacactgg
     3721 ttacgtaccg gaacactatt agagtatgtt atggctctgt tacagtttat aggtatgaaa
     3781 aaatataatc tttcctgtat gaaataatat tcttatggtg tgtggggtat ggtctgtata
     3841 ccatcatttt tatggtgaag ttaaatatct gtagaggcct caagctgggt cgctgttttt
     3901 acttaatggt acagaagtat taatagcggt gttgcctgtg gctacccgat actggctttt
     3961 gtgaatgtca gattacttcc tgtaagtgat tgaatggtac ttccttaaac tcaattctat
     4021 gaggtgtgtt tttaccattg taattcgtac tggtcctcag tttaagtgag tcttactgta
     4081 ctctgaaata ttacctcttt ggagtaatga ggtaatacga aacagtttta tcaggttgtc
     4141 tgtgctgctg gttacctcgt ttattaatgc cgcggtacgg cgtttctgta ctctcagtat
     4201 ttatgtaatt ccaccttcct gtaactttta taaagtaagg actataaaat gtataatgct
     4261 aatcccaatt acgaaatgga cttcatgatt ctgaaagacg tgaatgaaca catggaaggt
     4321 ctgtttcaac gtttttctaa actgttacct ttcagaattg attttgccta caggaaagac
     4381 acgcccagtt ttggtcattc ctgtaggcac agtatgtgta ttgaaatgca ccgcttactt
     4441 agtgaaaccc aaacgatgct ggcgggatat tactgggtaa tggaatatac gtcaaacaag
     4501 ggacttcata ttcattttat cggctacctg gatgggcaac gtcacaacaa atcttatcgg
     4561 atatccaggc aactgggaga tatctggagg cggatcacgg aaggggaggg ttattttcac
     4621 ctgtgcaggg ctaaagacaa atacccggtg cgtatcgatc acgtaattca ctatagtgat
     4681 aaatctgcgg tagacgactt acgttacgcc cttagctatc tggccaaaca ggatcagaaa
     4741 gaacacggta ttattctgag gcgtagcagg cttcccgaaa aaagtaaccg gggccgacca
     4801 cgacataact gaattacagc tgtattaggt attagaatta agtttctgta agttgttggg
     4861 aggtgcttga gttaataggc agaagagggc ggggcgcttt gtcatcagtg taggggaccc
     4921 gaatttctgc cgtgg
//
LOCUS       DAZFKM010000226         4907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFKM010000226 DAZFKM010000000
VERSION     DAZFKM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..307
                     /locus_tag="ACHKAY_004949"
     CDS             <1..307
                     /locus_tag="ACHKAY_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5703455.1"
                     /translation="KEIVISAPDGAVFRYDADAGALSASGMKTATLQASVSVKLDTPV
                     VECTNLLRTATLDVTKGGKMSGNITHSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            304..654
                     /locus_tag="ACHKAY_004950"
     CDS             304..654
                     /locus_tag="ACHKAY_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO5703456.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILITPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            658..1554
                     /locus_tag="ACHKAY_004951"
     CDS             658..1554
                     /locus_tag="ACHKAY_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO5703457.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLSEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            1547..2077
                     /locus_tag="ACHKAY_004952"
     CDS             1547..2077
                     /locus_tag="ACHKAY_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO5703458.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDTPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            2080..2793
                     /locus_tag="ACHKAY_004953"
                     /pseudo
     CDS             2080..2793
                     /locus_tag="ACHKAY_004953"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            3358..4212
                     /locus_tag="ACHKAY_004954"
     CDS             3358..4212
                     /locus_tag="ACHKAY_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703459.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     TSGAHAPAFIRSRRDTIDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALM
                     QGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGAHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNAENTVKNIA
                     FNYIVRLA"
     gene            4212..4790
                     /locus_tag="ACHKAY_004955"
     CDS             4212..4790
                     /locus_tag="ACHKAY_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5703460.1"
                     /translation="MTFRMSEQSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEADKLFRIREAEETKNSLMQVASEHIAPLQDAIDLDIATEEE
                     TSLLAAWKTYRVLLHRVDTTVAADIEWPVAPQ"
     gene            complement(4834..>4907)
                     /locus_tag="ACHKAY_004956"
     CDS             complement(4834..>4907)
                     /locus_tag="ACHKAY_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703461.1"
                     /translation="FINKDIPSNCTYITKKSGAVLYK"
BASE COUNT         1271 a         1235 c         1342 g         1059 t
ORIGIN      
        1 gaaagaaatc gtgatcagcg cgccggacgg cgcggtgttc cgctacgacg cggacgcagg
       61 cgcactgagc gccagcggca tgaaaacggc cactttacag gcatccgtca gcgtgaaact
      121 ggacacgccc gtcgtggaat gcacaaacct tctgagaacg gcgacgcttg acgtcacaaa
      181 agggggaaag atgagcggca atatcacgca cagcggcggc gatttcacct caaacggcat
      241 cacagtgcat acgcataagc acggtggcgt taaaggtggc agcgattcga caggaggccc
      301 gcagtgacaa cccgctacac aggaatgaat ccggacggga cgggaaacct gaacgatatg
      361 gagcacctga aacagtcagt cagggacatc ctgatcaccc cgctggcaag ccgggttatg
      421 cgacgggaat atggcagcct tgtgcctgat ttgattgacg aacccatgaa taacacaact
      481 cgtctgcaat gcatgagtgc tgccgtgatt gcgctgacac gatgggaacc ccgcattgcc
      541 ctggacgcca tcgacgttgt ctggaaggca ggaggccgcg ccggggtgac gctgtcgggc
      601 actgtcatgc agaccatgca gaatgttgaa ttaaccatca cgctgagaga gtaaatcatg
      661 cctgctgttg acctttccca gttaccggaa cccgccatca tcgcggagcc tgattttgag
      721 gcaattctgg ctgacacaaa ggccatgatg attgcgtcct atcctgccga acagcgtgaa
      781 gccgtctccg ccgcgctgga gctggaatcg gaacccctga acgttatcgc tcaaaccatg
      841 tcttttcgtg aaatgctgtt acgccagcgg gttaacgagg gcgcacgcgc ctgcatgtta
      901 agccacggtt cagggacaaa cctggacaac ctcgcgggca atatgaacac aaagcgcctg
      961 gttatcactc cggcaacgga taccaccgac gcggtgatgg agagcgacac ctcgctgaga
     1021 ttgcgggcgc aacgggcgta tgacggcctg agtgttgctg gcccgtcagg tgcatacgag
     1081 tattttgcac gcagcgccag cggtctggtg cgtgatgcgc gggccatcag cccgtctccg
     1141 gcctgtgtga cggtttccat cctgtccact gagggcgacg gcacagcaac ggaggcgttg
     1201 cttaataccg ttcgcgccgt tctgaatgca gaggataccc gcccggtggc cgaccgcctg
     1261 accgtacaga gtgccagaat cgtgacatgg cggctgaatg caaaactgta cttttacccc
     1321 ggcccggaat ccgaacctat tctggccgcg gcggaatcgt cgttcaggaa gtggctgtct
     1381 gagcaggggc ttatcggtca ggacgtggcg ttgtcagcca ttgctgccgc actgcatgtg
     1441 cacggtgtgc aacgcgtgga gataatcgaa cccacacaaa atatggccat cagcgacata
     1501 caggcggcgc gctgtgagtc attcaccatc agcgaaggtg ggcgtaatga gtaattcact
     1561 gttaccgcca tcagccagca atttcatgcg ttgtgccgaa gctgtcggaa cgcgcattac
     1621 agacactccg gtagacctca acacgctgtg gtcgccggac acctgcccgg tgcatctgct
     1681 gccttatctc gcctgggcat tttccgttga ccgctgggat cgcaactggc cggaagagac
     1741 aaagcgacaa gtgattcgtg atgcatggct gatacaccga cacaaaggga ccatcagcgc
     1801 actgcgccgg gccattgagc cgctgggata cctcattcgt gtgtctgagt ggtgggagtt
     1861 cggcggagaa ccgggaacat ttaccgttga agtcggcacg ctggacagtg gcgtgacgga
     1921 ggaaatgtat ctggaaatgg agcggttgat tgctgatgcc cgcccggtca gccgccacat
     1981 gacagggctg aatatcattc aggagatccc gggggatatt ttcgcggcgg cagcaactta
     2041 cgacggtgaa gtcattacca tttatccgga cgattaagca tgagtaccac aacacgaaaa
     2101 tttaaaacca ttatcactga tactggtgcc aaaaaattag ctcaggcagc cgcgccagat
     2161 ggtaatcctg tccgcctgac tcacatggcc gtgggcgacg gtggcggcac gttgcccaca
     2221 ccagacagta agcagacccg tctggtgcat gaggtgtggc gacatactgt taatcgcgtc
     2281 atcctggacg caacacatca gaaccgcatt attgcggagc tggttattcc tcctgaaacg
     2341 ggaggattct ggatccggga aattggtgtg tttgatgagc acggcgattt aatcgcggtg
     2401 ggcaatactg ccgaaagtta caagccagcc gttgccgaag ggtccggacg tgcacaaaca
     2461 tttcgcacca ttctgacagt atccagcact gccaccgtgg cgcttaccgt ggataacacc
     2521 atggttatgg ccacagtgga ttacgtggat aacaaactga aagagcatga acagtcacga
     2581 cgtcacccgg atgcctcgct gaccgcaaaa ggctttgttc aactcagtag cgccactaac
     2641 agcgattctg aaacgctggc tgcaacgccg aaagcggtta aggtcgcgta tgatcttgct
     2701 aacggaaaat atacagcgca ggatgccaca acagcgcgaa aaggccttgt ccagcttagt
     2761 agtgcaacca acagtacatc tgaaacgctg gcggcaacat caaatgcagt aaaagctgcc
     2821 tatgacaatg ctgaaaaacg tctgcagaaa gctaagaatg gtgaggatat ctctgacaaa
     2881 gacaccttta cgaaaaatat cggtgcctgc cgtgcatata gtgcagagct gaatattggt
     2941 ggagatagtg aagcatggac aactgcgcag ttgatttttt ggctagagag tcagggggca
     3001 tttaaccatc cttactggat gtgcaaaggc tcatgggctt atgcaagtaa taaggtcatt
     3061 acagatacag gttgcggaaa tatttgtctt gcaggtgctg ttgtggaagt tattggcacc
     3121 cgcggcgcaa tgaccatacg cattaccaca ccgagtacat ccagcggtga aggcatccct
     3181 aatgctcaat ttacttatat taatcatggt gatgcttatg ctcctggctg gcgaagggac
     3241 tataactcca ggaataagcc aacagcatca gagatcgggg cgttaccgtc agatgggaca
     3301 gcagtatcgt cagttaatct ggcttcaaaa ggtcggctga ccgccctgac agataatatg
     3361 cagggggcca caggtctgga gttatacgag gcgtataaca acggatatcc aacaacgtat
     3421 ggaaatatca ttcacctgaa agggatgaca gccgttggcg aaggtgagtt actcatcggc
     3481 tggagtggta caagcggtgc tcatgctccg gcatttattc gttcacgacg ggatacgatc
     3541 gacgcaaact ggtcgccgtg ggcgcagctt tacacctcgg ctcatcctcc tgcagagttt
     3601 tatccagtcg gtgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg
     3661 atgcaggggc agacttttga caaatctgca tacccgaaac ttgcagccgc ttatccgtca
     3721 ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgggcc
     3781 gtattgtctc aggaacagga cggcataaaa tcgcacaccc acagcgccag cgcatccagt
     3841 acggatttgg ggacgaaaac cacatcgtcg tttgattacg gtactaaaac gaccagttca
     3901 tttgattacg gcacaaaaac cacaaatagc gcaggagctc attcacacaa tatacctgtt
     3961 ggtcacactg gcgcggggaa tggtgtatca gccggttata acgctgcgtt aggtactggt
     4021 accacgtcga gcgcaggaga gcatgctcac aatgtatata tcggtgccca taaccacact
     4081 atcggcattg gtgctcatgc ccattctgtc attattggtc cccacggaca caccatcacc
     4141 gttaatgcta cggggaacgc agaaaacacc gtaaaaaaca tcgcatttaa ctatattgtg
     4201 aggcttgcat aatgacattc agaatgagtg aacaatcacg gaccataaaa atttataatc
     4261 tactgaccgg aaccaatgag tttattggtg aaggtgatgc atacattcca cctcatacag
     4321 gtctgcctgc aaacagtacc gatattgccc cgccagatat tccggctggc ttcgtggccg
     4381 ttttcaacag tgatgaggca tcgtggcatc tcgttgaaga ccatcggggt aaaacggttt
     4441 atgacgtggc atcaggggac tcgttattta tttctgaact cggtccgtta ccggaaaatg
     4501 ttacctggtt gtcgccgtat ggagagtatc agaagtggaa cggcacatcc tgggtgaaag
     4561 atgcagaagc agacaaacta tttcggataa gggaggcgga agaaacaaaa aacagcctga
     4621 tgcaggtagc cagtgagcat attgcaccac ttcaggatgc tatagattta gatattgcga
     4681 cggaggaaga gacatcgtta ctggctgcat ggaagacata tcgagtattg ttgcatcgtg
     4741 ttgatacaac agtagcagca gatattgagt ggccagtcgc cccacaataa aaagaaaaag
     4801 ccatcgatag aaatatcgat ggctttatgt actctattta tacaatacag caccgctctt
     4861 tttagttata tatgtgcagt tcgatggtat atctttattt ataaaag
//
LOCUS       DAZFKM010000227         4867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFKM010000227 DAZFKM010000000
VERSION     DAZFKM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..1129
                     /gene="hemB"
                     /locus_tag="ACHKAY_004957"
     CDS             155..1129
                     /gene="hemB"
                     /locus_tag="ACHKAY_004957"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO5703462.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHKAY_004958"
     CDS             complement(1235..2086)
                     /gene="tauD"
                     /locus_tag="ACHKAY_004958"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO5703463.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2083..2910)
                     /gene="tauC"
                     /locus_tag="ACHKAY_004959"
     CDS             complement(2083..2910)
                     /gene="tauC"
                     /locus_tag="ACHKAY_004959"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO5703464.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2907..3674)
                     /gene="tauB"
                     /locus_tag="ACHKAY_004960"
     CDS             complement(2907..3674)
                     /gene="tauB"
                     /locus_tag="ACHKAY_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO5703465.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3687..4649)
                     /gene="tauA"
                     /locus_tag="ACHKAY_004961"
     CDS             complement(3687..4649)
                     /gene="tauA"
                     /locus_tag="ACHKAY_004961"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO5703466.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1120 a         1407 c         1244 g         1096 t
ORIGIN      
        1 tcagtgaata tgaaattatt gctatttcta ttacaaagat gtcctggtct ctctttcatt
       61 ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct tcaggtatac
      121 tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc aacgccctcg
      181 tcgcctgcgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa
      241 cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag ccgttgaagc
      301 catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg aacgcatcgc
      361 caacgccggt atccgttccg taatgacttt tggcatctct caccataccg atgaaaccgg
      421 cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct gcaagcagac
      481 tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt ctcacggtca
      541 ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgca actctggaaa atttaggcaa
      601 gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg ccgcgatgga
      661 tggccaggta caggctattc gccaggcgct ggacgctgca ggctttaaag atacggcgat
      721 tatgtcgtat tcgaccaagt tcgcctcttc cttttatggt ccgttccgtg aagctgccgg
      781 aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc
      841 gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg ttaaacctgc
      901 tggagcatac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc
      961 gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg gtgctataga
     1021 tgaagagaaa gtcgtgctcg aaagcttagg ttcgattaag cgtgcgggtg cggatctgat
     1081 tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat ttttttcctc
     1141 tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc ctaaccctct
     1201 ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa aacggtttat
     1261 ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg gcattggcat
     1321 agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag cgccaacgca
     1381 cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct tcgctctctt
     1441 tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc gcctgtttac
     1501 cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc gcgaccgcct
     1561 cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg aacgatttac
     1621 ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca gagagcgcct
     1681 cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt aactctttag
     1741 ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta tgccagttgt
     1801 cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca accccttcgg
     1861 catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct
     1921 gctgcggcgc aatagtttga tcgcgcagaa acaccacctg atggcgcagc accgcatggt
     1981 aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg
     2041 cgccgatata cggccccagc ggggtaatgc tcagacgttc actcattgta cttctccatg
     2101 ccagggcgtc agacggcgct gtaacgcgcg caaacccagt tctaaaagaa aggcgataat
     2161 cgcaatcacc gcgatccccg ccagcaccac gtcagtcgcg agaaattcac ccgctgactg
     2221 aaccataaat cctaaaccgc gcgtcgcggc aatcagctcc gccgctacca gcgtagacca
     2281 gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat
     2341 gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac gaatgcgaac
     2401 ctgctgcgcg cttttcaccc ccgccagcgc cgacatcgcc accggtgcaa aaatcgctaa
     2461 atagatcagt aagatcttcg aggtttcacc aataccaaac cagatcacca tcagcggcaa
     2521 ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa tgccgcgcac
     2581 cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg ccgcgagtag
     2641 cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg cgtccataaa
     2701 tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa
     2761 cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta aaaccgctaa
     2821 cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca gccgccgcga
     2881 atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc aaatacgcgg
     2941 cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct gcggctcgac
     3001 tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac gcggccaggg
     3061 ccgggtgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat atcgtgggta
     3121 atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagggt ttgcatctgg
     3181 tcgcgggtga aggcgtccag cgcaccaaac ggctcgtcga gtaataacag ctggggattc
     3241 gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag ctgccagata
     3301 tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc gatttccagt
     3361 cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc ctgtacattg
     3421 cgccacggta gcagcccttc attctgaaaa accaccccgc gctctgctcc cggtccctca
     3481 atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc ggcaatcaga
     3541 ttcagcaggg tggttttacc gcagccggac ggccccagca ccaccagtag ctcgccgctt
     3601 tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc gtaaagatga
     3661 gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac gtactggctg
     3721 taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg
     3781 atcgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt cagataggta
     3841 ttccccttca ccagccccgg aacgtcacct tccggcacgc cgcttaaacg cgccagtttg
     3901 ctgatgtttt ccggctgttt cagccacgcg tctgggttag caatgtacgg ttgctgagca
     3961 tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc aaaatctttg
     4021 cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc agaatcggtc
     4081 agcaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata agcaccatca
     4141 atatctcccc gctgccaggc agcgataatc gcgggcggct gcaggttcac aatctccact
     4201 tgcccgggtt taatgcccca gtgtttcagc gccgccagca ggctgtagtg ggtggtagag
     4261 ataaacggta cggcgatgcg tttgccaatc agatcttccg gtttgctgat agttttcttt
     4321 accaccagcg cttcggagtt acccagtttt gacgccagca agaaaacttc aatcggcacc
     4381 tgttggctgg ctgcaaccgc taacgggctg gaaccgaggt tgccgatttg cacatcgcca
     4441 gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc cacggttgct
     4501 ccgctttctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc ggctgaggtt
     4561 tgatacgcaa cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca
     4621 agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt gttatgtgag
     4681 cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac agatgatttt
     4741 attcttagca aaaaaacggc aaaggaaaga ttaaatatgc aattactccg ttctaacaat
     4801 tgggaaaatt actacacttc attttagtga tgcataaaca accacattac gagatttatt
     4861 acttcat
//
LOCUS       DAZFKM010000228         4805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFKM010000228 DAZFKM010000000
VERSION     DAZFKM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..1340
                     /locus_tag="ACHKAY_004962"
                     /pseudo
     CDS             9..1340
                     /locus_tag="ACHKAY_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1353..2312
                     /locus_tag="ACHKAY_004963"
     CDS             1353..2312
                     /locus_tag="ACHKAY_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO5703467.1"
                     /translation="MKWLLFITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2351..3250)
                     /gene="nhaR"
                     /locus_tag="ACHKAY_004964"
     CDS             complement(2351..3250)
                     /gene="nhaR"
                     /locus_tag="ACHKAY_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO5703468.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3316..4482)
                     /gene="nhaA"
                     /locus_tag="ACHKAY_004965"
     CDS             complement(3316..4482)
                     /gene="nhaA"
                     /locus_tag="ACHKAY_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO5703469.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1456 a         1084 c         1004 g         1261 t
ORIGIN      
        1 cataatcggg tacgatttta tccgataact ataatgctat aactctggga aatggttgga
       61 atacaccgct gggtgccatc tctttttacg ctgcccgatc gagtagtaag ttaaataatg
      121 ataccagaca cgaaggtacg agctatcagg tcgcctataa taaatatcta gtacaaacag
      181 ccactcgatt tagcgtggcc gcctggcgtt atgcttcgca ggattacagg acattcagcg
      241 accatcttta cgaaaatgat aaacactatc atcagagtga ttatgatgat ttttatgata
      301 ttggcagaaa aaatagcctt tctgctaata ttatgcaacc tttatccaat aatctgggaa
      361 atgtatcatt aagtgcgctt tggcggaatt actgggggcg aagtggaaat gctaaagatt
      421 accaattcag ttactccaat aactggcaac acattagtta tactttctct gccagccaat
      481 cttatgatga aaataataaa gaagaggagc gttttaatct gtttatctcc attcctttct
      541 actgggggga tgatattgcc aaaacacgtc accaaattaa cttatcgaat tcgacctcat
      601 tttccaaaga tggctattcc tccaacaata ctggaattac tggcatagcc ggtgaacatg
      661 atcagttaaa ttatggtata tatgttaatc agcaacaaca aaataatgat acctcgcttg
      721 gtacgaattt aagctggaga actcccatcg ccataataga tggcagctat agtcattcta
      781 aaaacgcctg gcaaagtggt ggaagtatta gtagtggatt agttgtctgg cccggtggta
      841 ttaatatcac taaccagtta tccgatacat ttgcaattct ggatgcgcct gggttagaag
      901 gtgcacatat taatggacaa aaatacaatc gaacaaacag caaaggccag gttgtttacg
      961 acctgataat acctcatcgt gaaaaccatc tggtacttga tatagcaaac agtgaaagtg
     1021 aaacagaatt gcagggcaat cgtcaaatta ttgcacctta ccgtggagca gtttcttatg
     1081 tgcagtttac aactgaccaa cgtaagccct ggtatataca ggcactgcgt cccgatggtt
     1141 cgccattaac ctttggctat gacgtactgg atctccagga aaataatatt ggagtcgttg
     1201 gccagggtag tcgccttttt attcgcgtag atgaaattcc cactggcata aaagttgctc
     1261 tcaatgatga acagaactta ttctgtacta ttacttttca acacgttatc gatgaaaaca
     1321 aaacatatat atgccagtaa aaggagttta ctatgaagtg gcttttattt atcaccttga
     1381 gtctatattc ttttattgtg caaagtgctc cctgtgcatt gaccaatgtc ggtgaacaac
     1441 gtggaacata tatactcaag ccactgagta tgaaaggtaa tctaaccgcc gaaaaaattg
     1501 aaaacaccgg aaatatcatt gagtgtacag acttgcaaga gacaaccaac gccattaaag
     1561 ttcatttttt aacacagaac gctttaaggt ttaaagtcgg aagtcgtcat tatattatta
     1621 atttcccctt tgaatctgac ggtaaaaaaa tagagcttcc agggaagtca tcttataatt
     1681 tagaagaaat gctatctgcg ataaatttca ccattccata ttctatcgca tctgtatcag
     1741 atagttctaa caccgcagac atttttcctg gaactccttt tcctttgttg ttcgaaattg
     1801 aagtgagttc atgtacaggg aataatacta attgtaatag cgtccgcttt aattataatt
     1861 taaatactat tttacaggta aatttaatga cctgtggttt tgaagatcaa tccatcgaca
     1921 ctggacgatt tgtactgtct cgtatcaata atgaaaatta tcagtttcat catataagat
     1981 ttgactgcat ccagggcgag caaggtgaat tagtttttga acccagtgat gttgaatatt
     2041 actttgaacc agtgacgata caggagtcag gaaccagcat attaaaaaat gagttagaaa
     2101 acagtgagga cggtgcgggt caaataggct ttcaactctc caatgatggt acgaatgaaa
     2161 tccagtatgg taaaagcaat tactactctt ttcagcatcc ccatgaagga agtaatcaga
     2221 ttcctctatt tattcgcccg cgtacatatg gaaataatgt ctcaagcggg caaataatga
     2281 gccgagtcaa aatcgtggtt atgtacaact aatctatctc ccccacctta actttggggg
     2341 gaggtgccga ttaacgcgcc gctggactaa aaagcgcaga ataatccgta ttgcagattc
     2401 gctgtaccgc cgggtgctga atcatccgct cagcaaaaat agcatggtac tcttccatca
     2461 cattctcgac gcgaccaatt tctacgacag ttttatcggc ataaaagtca tatgcataaa
     2521 gcgttggggc aacgaagatt gcattgtgca tcgcaccaaa agctttcatc aacgcggcat
     2581 catcaaactc gccgaggatt tctacgttta atccctggga gttaaaccag ttaagcaatt
     2641 tgcgccctaa cattgaacgt cgcccaggaa tcaaaagtcg ccgttcttcc agacaagccg
     2701 ggaacggttt ttctggtggt ggatttgtac accagaaact cacgccacat tcgccaattc
     2761 tcacggagaa caggccttcc tgctgcgtag agtctatcgg acagtcagaa atgatcatat
     2821 ccagtttatg ctgacttaat tgctccagca gcatttcgtg ggtggattcg aagcagcgaa
     2881 gatgaatggg ctcgccttct accactgcgg cgttaagtac gctactgacc aggcgtttgg
     2941 aaagtgcatc agccacacca acgtcaaaca ataaattgga ttctttgcga tagttcacaa
     3001 tatccagcat ttcctggctt aaggtgaaca ttttatcggc atagcgatag accagttctc
     3061 ccagctcgct gggttcgaga ccacgtccct tgcgtttaaa taatttgcct tgcaggcgct
     3121 cttccagcgc tcgaatctgt ccggtaatgg tttgtggagt taaataaagc gcctccgctg
     3181 cgccaaccac ggaaccttct ttatagacat gccagaagta atacaagtgg ttgtaattga
     3241 tatgagacat gctcatttct ctccctgata acaatgaaaa gggagccgtt tatggctccc
     3301 cggtaaaccg tcctgtcaaa ctgatggacg caaacgaacg cgtaaccagc tgtatccaat
     3361 taccgccgaa gagatagaac cgaccaggat accgagtttc gcccagttaa tcagttctgg
     3421 atctacgcta ccaaaggcca ggctggcaat aaagatagac atagtaaaac cgataccgca
     3481 caggatcccc accgccataa tttgctgata agtcgttccc tcaggcagat gcgccagttt
     3541 caaacgcagc gccaaccagc agaacagact aatccccagc ggtttgccaa tcagcaagcc
     3601 agcgatgatc cccaatggca gaatggaggt caagccatcc agcgtgacac cttgcagtga
     3661 aacgccagca ttagcaaatg caaacagcgg caaaatcaga tacgccaccc acgggtgtaa
     3721 cacatgctcc agtcgcttcg ctggagaacg cccatgcttc tctttcaaag gaataaagaa
     3781 gccgacaatt acccccgcca gggttgcgtg aacccccgat ttcaacaccg cagtccacaa
     3841 cactacgcca accagaatat agacgcccgt gcggcgtaca ccacacagat tcaataccgc
     3901 gagtaccgca attgctacag ccgcgacgcc aagagaggcc atcgataagt cattagtgta
     3961 gaacaatgcg atgataatga tggccccaag atcgtcgata atagccagag ccatcaaaaa
     4021 gatcttcagc gctaacggaa cacgacttcc caacagcgcc agcacaccaa gtgcaaaggc
     4081 aatgtcagta gccgccggga tcgcccaccc ttcgcgggta atcggatcgg catagttaaa
     4141 ggccagatag agtaatgccg gaacaatcat cccaccaata gcggcgataa ctggaaatgc
     4201 ggcctggcgt aagctggcta gcgatccttg catcagttca cgtttaactt ccagaccgac
     4261 taacaggaaa aataccgcca tcagcgcgtc atttatccat aacagcatgt ttttgttgat
     4321 ttcgagtgaa ccaacccgga gctgaaccgg cgtctccaga aagtcgtgat accatccact
     4381 ggttgcgccg ctattggcca taatcatcgc caggatagcg gcaatgataa gaataatgcc
     4441 tcccgaggca tcactgctaa agaatcgatg cagatgtttc actttttatt tctctttcag
     4501 gtgaatagat cgtttttact attttacccc gcacaaatca tcgttaaaat cagattatag
     4561 tgtaggaata actcgttttt tacgatccat tgaagaattt gcgccatcag cggtgagatg
     4621 agtttttgac ggctgtaagc gctcacgagt gaccctaagc ccagataatc atagcttaag
     4681 gtgaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
     4741 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaaga
     4801 gataa
//
LOCUS       DAZFKM010000229         4731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFKM010000229 DAZFKM010000000
VERSION     DAZFKM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            330..1307
                     /locus_tag="ACHKAY_004966"
                     /pseudo
     CDS             330..1307
                     /locus_tag="ACHKAY_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1830..>4731
                     /gene="efa1"
                     /locus_tag="ACHKAY_004967"
     CDS             1830..>4731
                     /gene="efa1"
                     /locus_tag="ACHKAY_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO5703470.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEIIEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENIFKNIEMAIADIKKEYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQNTPQKNIDFINLYNE
                     IRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFFLENMIKLQDNFFNYCIV
                     KGVTEINDELRINYLKNVIKLSDDDIGNYQKTINDNKDRVKKLILDLQKQFGENRISI
                     KDVNSLTSLSKSENNHNYQTEMLLRWNYPAASDLLRMYILKEHGGIYTDTDMMPAYSK
                     QVIFKIMMQTNGDNRFLEDLKLRRAISDGVLRYVNNQNIDEVNYNEISDADKNIIKKI
                     LTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDA
                     VIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGILVKKYLSGSLFSKFR
                     QDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKLSDKAYIGVYKTTGT
                     GKYDWLNPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSKLKTENLPELFLTKIDT
                     DTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSSTIDFKNLYEIDQMLHMIMLEMNDDI
                     AKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLHKKNDNDLSMSIKGFLASNP"
BASE COUNT         1664 a          890 c          926 g         1251 t
ORIGIN      
        1 tatatgagaa caccaataag aataataata tttgatcaca actgaagatg ctttttacaa
       61 tgaagaatta ttgttaaaaa acgctttgtt gaatcaaccg aactgttgct gagttgcagg
      121 atcacatcac gtattttcct gaaaacgcag gctgcttagt agcaaagcag aagttcaaaa
      181 tcaccaacta aatagctgcg ccgaatagta gatcaatgaa agggaactca gcccggattg
      241 tgcgatctga tcaatcgcca aaaatccccc cctaaccgga ctgagcgatg ccgatcatag
      301 caccaattcc ccgcgacgaa tgacgcctga tgcaaaaagc aatctataaa actcacgata
      361 aaaattatgc ccgcaggctg accgccatgc tgatgctgca ccggagcgct cgcgtcagtg
      421 acgttgccag aacgctctgc tgcgcccgtt cctccgtcag gcgctggatt aactggttca
      481 cgctgtcgag tgttgaaggt ctgaaatcat tacccgcagg acgttcccgc cgctggccgt
      541 ttgagcatat ctgcacattg tttcgtgaac tgataaaaca ctctcccgga tattttggct
      601 atcagcgttc acgctggagt acagaactgc tggcaataaa aatcaacgag ataaccgagt
      661 gccagttaca tgccggaacc gttcgccgct ggttgccatt agtcgggctt gtgtggcgca
      721 gggctgcgcc aaccctgcgt acccgtaacc cgcataaagc agcaattcac aaagcactgg
      781 acgaatgtag tgcggagcat ccggtctttt atgaagatga agtgtatatc tatcttaatc
      841 ccaaaaccgg tgcggactgg caacttcgca gacagcaaaa acatgtggta acaccggggc
      901 agaatgaaaa atactatctg gctggtgctt tacatagtgg aacgggcaaa gtcagctatg
      961 tgggtggcaa cagcaaaagc tcagccttat ttatcagtct gttgaaacat ctgaaattga
     1021 cataccgacg ggataaacct atcacgctca tagtggacaa ctacatcatc cataaaagtc
     1081 gggaaacaca gcgctggctg aaagaaaatc cgaagttcag agtgatttat cagccagttt
     1141 actcgccgtg ggtgaatcat gttgagcggt tatagcaggc gcttcacgat acgataacgc
     1201 gcaatcatca gtgccgttca atgtggtaac tgcttaaaaa ggttcattat tttatggaaa
     1261 ccgtgagccc attccccggt ggtaaatatg ggctgttaaa agtgtagcgg tattcggcgc
     1321 agctatttag gatggtcagg tcgcagaagt tctgtgccac tacagtgcgt gtactaaaca
     1381 aaatgacgag ggcacgaatg tcataatacg tgcgtattac ctgcagtagc taccaactga
     1441 gggggcttat cccaaacctg atttattcaa caaagctgga ctggacttat ccttactctg
     1501 aattattttc cagacatatg catcatgtac tggctcgctt ggttcaggaa atacaggagc
     1561 aaatctacag ctattagatt tattcctatt tgtatgcaaa gtcgcattgc ataatgcgga
     1621 gctatgttac agatacgcct gacgtgcgta gtaaaattat aaagcaacat atatatacaa
     1681 ggagaaaccg agaaaaataa atgagtcgta tgttgggtag catcgagata aatatttttt
     1741 tatcacatta gcaccttggt taagggtgtt aataataaga ttttctcact tattaaaaca
     1801 taaataattt tttaagcgga aatgtaatta tgagactgcc agagaaagtt ctttttcctc
     1861 ctgtcactag tggcctgtca gggcaggaaa aacaaaaaaa accgaagagc attaccggat
     1921 ttcaggaaaa ttatcaacgc aatatcaggc caatcaaaac agcatcagaa gcccgactac
     1981 gcttctttga taaaatggtt tcgaaagaaa actctctgga agatgttgtt tctttaggtg
     2041 aaatgattca gaaggaaatt tatgggcatg aacaaagaac attttcacca gttcatcata
     2101 caggtaactg gaaatcatca ttgttacaca acgcgctcct tggtctggca aatgtttata
     2161 atggcttacg ggaaacagaa taccctaaca ctttcaacag agatggtata aaaagtacta
     2221 actcttttag agataactta ttgacaaaaa caagaactcc cagagataat tttgaggaag
     2281 gaataaaaca tcctgaacat gcaacaatac catatgacaa cgacaatgaa agtaataaat
     2341 tgctaaaagc aggaaagata gctggtaaca ataacgagct gttgatggaa ataaaaaagg
     2401 aatccaaaag cgaccatcaa atccccctgt cagataagtt cctgaaaagg aaaaaacggt
     2461 ctcctgtagc tgaagataaa gttcaaaact cgttaacacc agaaaatttt gttcagaaaa
     2521 tttcacttag tgatgagctt aaaacaaaat atgcaaatga aattatagag ataaaaagaa
     2581 taatgggaga atacaatctt ttaccggata aaaacagtcg taatggtcta aaacttctac
     2641 agaagcaagc tgatttacta aaaataatca tggaggatac ctctgttaca gaaaatatct
     2701 ttaaaaacat agagatggct atagcagata taaaaaagga gtactattcg catacagttg
     2761 atattgagaa aaatattcat gccatatggg ttgcgggttc cccacctgaa agcatctcgg
     2821 actatattaa gacttttctc aaaacctaca aagagtttac ttactatctt tgggttgatg
     2881 aaaaagcatt tggagccgcg aaatttacca gtgtcttaaa acaaattgca tttgatttag
     2941 catgcagaac tatacagcag aatactccac aaaaaaatat tgattttatt aatctatata
     3001 atgaaataag gaagaaatac aacaacaacc catcgggaca acaagagtac ttaaacaaac
     3061 tcagagagct ttatgctact tatcaaaaaa tatctacccc tctgaaacac atgtttaatt
     3121 cattttttct agaaaacatg attaaacttc aggataattt cttcaactat tgcattgtca
     3181 aaggtgttac agagattaat gatgagttac gaataaacta ccttaaaaat gtaataaaac
     3241 tgtcagacga tgacattggt aattatcaga aaacaattaa cgacaataaa gatagagtaa
     3301 aaaaactaat tcttgattta cagaaacaat ttggtgaaaa ccgtatttca attaaagatg
     3361 ttaactcttt aacctctctt tcaaaatcag aaaataatca caattatcaa actgaaatgt
     3421 tgcttcgatg gaactatcct gccgcctcag acctgctcag gatgtatatc cttaaagagc
     3481 atggtggtat ttatacagac acagacatga tgcctgcata ctctaaacaa gtaattttta
     3541 aaattatgat gcagacaaac ggagataatc gtttcttgga agatttgaaa ctacgtcgtg
     3601 caatatctga tggtgtatta agatatgtta ataaccaaaa tattgatgaa gttaactata
     3661 atgaaatcag tgatgcagat aaaaacatta tcaagaagat attaacagaa atatctaaaa
     3721 tgccagaaga tagcattttt actaagatca atacaaggat tcctcgagac acaatgccca
     3781 tccttcgtcg ttatcaccta tggcctgatg gatggaatat tcgtgggctc aatggattca
     3841 tgctatcaca taaaggtagt gaagtgattg atgctgttat cgcaggccag aatcaggctt
     3901 acagggaact aagaagaata agagataata ttcatagtga aatatacttc aaacaaactg
     3961 atgaattgtc ctcacttcca gatacagaca aaattggagg gattctggta aaaaaatacc
     4021 tttcaggaag tcttttttca aaattcagac aagatactat tatcccggag gcattgagca
     4081 ccctccagat atcaggtcct gacttgattc aaagaaagat gttgcaattt ttcaggagta
     4141 gaggggtgtt aggtgaagag ttcattaatg aaagaaaact gagtgataaa gcttatattg
     4201 gtgtctacaa aacaactggc acagggaaat atgactggtt aaaccctgaa tcgatcggcg
     4261 ttaatgatgt cacgcccgca gatgaaagta cctggtgtat aggaaaaggc cggtgtgttg
     4321 atgacttcct gttcaaagat gtttcaaaac taaaaacaga aaatcttcca gaattattct
     4381 taacaaaaat agatactgat acgtttttct ctcagtggtc aaccaaaacc aagaaagatc
     4441 tgcaaaaaaa aatacaagac cttactgtac gttataatga gctaattgac tcgtcgacta
     4501 tcgactttaa aaatctatat gaaatagatc aaatgctcca tatgattatg ctagagatga
     4561 atgatgatat agccaaaagg tcattgtttt cattgcaagt tcaaatagcc gaaaaaattc
     4621 ggaggatgac cattcctgta gacaatataa ttaacatcta tcctgatcta cataaaaaaa
     4681 atgacaatga tctgagtatg tccataaaag gctttcttgc gagtaatcca c
//
LOCUS       DAZFKM010000230         4688 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFKM010000230 DAZFKM010000000
VERSION     DAZFKM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1297
                     /locus_tag="ACHKAY_004968"
     CDS             <1..1297
                     /locus_tag="ACHKAY_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5703471.1"
                     /translation="RGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            1294..1500
                     /locus_tag="ACHKAY_004969"
     CDS             1294..1500
                     /locus_tag="ACHKAY_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5703472.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            1497..3098
                     /locus_tag="ACHKAY_004970"
     CDS             1497..3098
                     /locus_tag="ACHKAY_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5703473.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3079..4398
                     /locus_tag="ACHKAY_004971"
     CDS             3079..4398
                     /locus_tag="ACHKAY_004971"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5703474.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            4408..>4688
                     /locus_tag="ACHKAY_004972"
     CDS             4408..>4688
                     /locus_tag="ACHKAY_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5703475.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT         1053 a         1262 c         1460 g          913 t
ORIGIN      
        1 ccgtggctcc acgcccaaag tgagaggcac ctgccagatt gagcgtgcag ccagtgaatc
       61 cccgcatttt atgcgttttc atgttgcctg cccgcactgc ggggaggagc agtatcttaa
      121 atttggcgac aaagagacgc cgtttggcct caaatggacg ccggatgacc cctccagcgt
      181 gttttatctc tgcgagcata atgcctgcgt catccgccag caggagctgg actttactga
      241 tgcccgttat atctgcgaaa agaccgggat ctggacccgt gatggcattc tctggttttc
      301 gtcatccggt gaagagattg aaccgcctga cagtgtgacc tttcacatct ggacggcgta
      361 cagcccgttc accacctggg tgcagattgt caaagactgg atgaagacga aaggggatac
      421 gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag acgtgggaag cgaaaatcgg
      481 cgaacgtccg gatgctgaag tgatggcaga gcggaaagag tattattcag cgcccgttcc
      541 tgatcgtgtg gcttacctga ccgccggtat cgactcccag ctggaccgct acgaaatgcg
      601 cgtatgggga tgggggccgg gtgaggaaag ctggctgatt gaccggcaga ttattatggg
      661 ccgtcacgac gatgaacaga cgctgctgcg tgtggatgag gccatcaata aaacctatac
      721 ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc tgggatactg gcgggattga
      781 cccgaccatt gtgtatgaac gctcgaaaaa acatgggctg ttccgggtga tccccattaa
      841 aggggcatcc gtctacggaa agccggtggc cagcatgcca cgtaagcgaa acaaaaacgg
      901 ggtttacctt accgaaatcg gtacggatac cgcgaaagag cagatttata accgcttcac
      961 actgacgccg gaaggggatg aaccgcttcc cggtgccgtt cacttcccga ataacccgga
     1021 tatttttgat cttaccgaag cgcagcaact gactgctgaa gagcaggtcg aaaaatgggt
     1081 ggatggcagg aaaaaaatac tgtgggacag caaaaagcga cgcaatgagg cgctcgactg
     1141 cttcgtttat gcgctggcgg cgctgcgcat cagtatttcc cgctggcagc tggatctcag
     1201 tgcactgctg gcgagcctgc aggaagagga tggtgcagca accaacaaga aaacactggc
     1261 agaatacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgccgct
     1321 gcccgtgcgg cactgcatga cctgatgaca ggaaaacggg tggcaacggt acagaaagac
     1381 ggacggcgag tggagtttac gaccacttcc gtgtctgacc tgaaaaaata cattgctgag
     1441 ctggaagtgc agaccggcat gacacagcga cgcaggggac cagcaggatt ttatgtatga
     1501 aaatgtccac cattcccacc cttctggggc cggacggcat gacatcgctg cgtgaatatg
     1561 ccggttatca cggcggtggc agcggatttg gtgggcagtt gcgggcgtgg aacccaccgg
     1621 gtgaaagtgt ggatgcagcc ctgctgccca actttacccg tggcaatgcc cgcgcagacg
     1681 atctggtacg caataacggc tatgccgcca acgccatcca gttgcatcag gatcatatcg
     1741 tcgggtcttt tttccgactc agtcatcgcc caagctggcg ctatctgggc atcggggagg
     1801 aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaagatg
     1861 actgttgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg
     1921 tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat acccgtccct
     1981 cgcgactgtt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca
     2041 ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc
     2101 tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga
     2161 taccccgcga gttacccggc ggtcgtgctt cgttcattca cgtctttgaa cccgtggagg
     2221 acgggcagac ccgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg
     2281 acacgctgca gaacacgcag ctgcagagcg ccattgtgaa ggcgatgtat gccgccacca
     2341 ttgagagtga gctggatacg cagtcagcga tggattttat tctgggcgcg aacagtcagg
     2401 agcagcggga aaggctgacc ggctggattg gtgaaattgc cgcgtattac tccgcagcac
     2461 cggtccgtct gggaggcgca aaagtgccgc acctgatgcc gggggactca ctgaacctgc
     2521 agacggctca ggacacggat aacggctact ccgtgtttga gcagtcactg ctgcggtata
     2581 tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg gaattacgcc cagatgagct
     2641 actccacggc acgggccagt gcgaacgagt cgtgggcgta ctttatgggg cggcgaaaat
     2701 tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg gctggaagag gccatcgttc
     2761 gccgcgtggt gacgttacct tcaaaagcgc gcttcagctt tcaggaagcc cgcagtgcct
     2821 gggggaactg cgactggata ggctccggtc gtatggccat cgatggtctg aaagaagttc
     2881 aggaagcggt gatgctgata gaagccggac tgagcaccta cgagaaagag tgcgcgaaac
     2941 gcggtgacga ctatcaggaa atttttgccc agcaggtccg tgaaacgatg gagcgccgcg
     3001 cagccggtct taaaccgccc gcctgggcgg ctgcggcatt tgaatccggg ctgcgacaat
     3061 caacagagga ggagaagagt gacagcagag ctgcgtaatc tcccgcatat tgccagcatg
     3121 gcttttaatg agccgctgat gcttgaaccc gcctatgcgc gggttttctt ttgtgcgctt
     3181 gcaggccagc ttgggatcag ccgcctgacg gatgcagtat ccggcgacag cctgactgcc
     3241 ggagaggcac ccgcggcgct ggcgttatcc ggtgatgatg acggaccacg acaggcccgg
     3301 agttatcagg tcatgaacgg catcgccgtg ctgccggtgt ccggtacgct ggtcagccgg
     3361 acgcgggcgc tgcagccgta ttcggggatg accggttaca acggcattat cgcccgtctg
     3421 caacaggctg ccagcgaccc gatggtggac ggcattctgc tcgatatgga cacaccgggc
     3481 ggaatggtgg cgggagcatt tgactgtgct gacatcatcg cccgtgtgcg tgacataaaa
     3541 ccggtatggg cgctggccaa cgacatgaac tgcagtgcag gtcagctgct tgccagcgcc
     3601 gcctcccggc gtctggtcac gcagaccgcc cggacaggct ccatcggcgt catgatggct
     3661 cacagtaatt acggtgctgc cctggagaaa cagggcgtgg aaatcacgct gatttacagc
     3721 ggcagccata aggtggatgg caacccctac agccatctac cgggtgatgt ccgggaaaca
     3781 ctgcagtccc ggatggatgc aacccgccgg atgtttgcgc agaaggtgtc ggcatatacc
     3841 ggcctgtccg tgcaggctgt gctggatacc gaggctgcag tgtacagcgg tcaggaggcc
     3901 attgatgccg gactggctga tgaacttgtc aacagcaccg atgcgatcac cgttatgcgt
     3961 gatgcactgg atgcacgtaa atcccgtctc tcaggagggc gaatgaccaa agagactcaa
     4021 tcaacaactg tttcagccac tgcttcgcag gctgacgtta ctggcgtggt gccagcgatg
     4081 gagggcgaaa acgccagcgc ggcgcagccg gacgtgaacg cgcagatcac cgctgcggtt
     4141 gcggcagaaa acagccgcat tatggggatc ctcaactgtg aggaggctca cggacgcgaa
     4201 gaacaggccc gcgtgctggc agaaaccccc ggtatgaccg tggaaacggc ccgccgcatt
     4261 ctggccgcag caccacagag tgcacaggcg cgcagtgata ctgcgctgga tcgtctgatg
     4321 cagggggcac cggcaccgct ggctgcaggt aacccggcat ctgatgccgt taacgatttg
     4381 ctgaacacac cagtgtaagg gatgtttatg acgagcaaag aaacctttac ccattaccag
     4441 ccgctgggca acagtgaccc ggctcatacc gcaaccgcgc ccggcggatt gagtgcgaaa
     4501 gcgtctgcaa tgaccccgct gatgctggac accgccaccc gtaagctggt tgcgtgggat
     4561 ggcaccaccg acggtgctgc cgttggcatt cttgcggttg ctgctgacca gaccagcacc
     4621 acgctgacgt tctacaagtc cggcacgttc cgttatgagg atgtgctctg gccggaggct
     4681 gccagcga
//
LOCUS       DAZFKM010000231         4670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFKM010000231 DAZFKM010000000
VERSION     DAZFKM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..412
                     /gene="grlA"
                     /locus_tag="ACHKAY_004973"
     CDS             <1..412
                     /gene="grlA"
                     /locus_tag="ACHKAY_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFO5703476.1"
                     /translation="GSKNKNGDYVIPDSVKNYDGEPLYILVSLWCKLQEKWISRNDIA
                     EAFGINLRRASFIITYISRRKEKISFRVRYVSYGNLHYKRLEIFIYDVNLEAVPIESP
                     VSTGPKRKNYRVGNGIVGQSNIWNEMIMRRKKEN"
     gene            complement(798..1253)
                     /gene="cesD"
                     /locus_tag="ACHKAY_004974"
     CDS             complement(798..1253)
                     /gene="cesD"
                     /locus_tag="ACHKAY_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO5703477.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
     gene            complement(1267..2805)
                     /gene="escC"
                     /locus_tag="ACHKAY_004975"
     CDS             complement(1267..2805)
                     /gene="escC"
                     /locus_tag="ACHKAY_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO5703478.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISNSVNDDFSGEIKNEKPMKVLEKLSRLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLRDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNANNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2805..3260)
                     /gene="sepD"
                     /locus_tag="ACHKAY_004976"
     CDS             complement(2805..3260)
                     /gene="sepD"
                     /locus_tag="ACHKAY_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO5703479.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPPTHLFSDIGRIV"
     gene            complement(3266..3838)
                     /gene="escJ"
                     /locus_tag="ACHKAY_004977"
     CDS             complement(3266..3838)
                     /gene="escJ"
                     /locus_tag="ACHKAY_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO5703480.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3840..4217)
                     /gene="sctI"
                     /locus_tag="ACHKAY_004978"
     CDS             complement(3840..4217)
                     /gene="sctI"
                     /locus_tag="ACHKAY_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO5703481.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSIQ"
     gene            complement(4301..4603)
                     /locus_tag="ACHKAY_004979"
     CDS             complement(4301..4603)
                     /locus_tag="ACHKAY_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO5703482.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMTPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
BASE COUNT         1355 a          865 c          889 g         1561 t
ORIGIN      
        1 gggatctaaa aataaaaatg gcgactatgt aattcctgac tcagtaaaga attacgatgg
       61 tgagcctctg tatatcttgg tttctctttg gtgtaaattg caggagaaat ggatttctcg
      121 caatgatatt gccgaagcat tcggtataaa cctgaggaga gcatcattta ttataactta
      181 tatatcgaga agaaaagaaa aaatttcatt tcgtgtcaga tatgttagtt atggtaactt
      241 gcattataag cgccttgaga ttttcattta tgatgttaac ctcgaggctg ttccgataga
      301 aagtccggta tcaaccggac caaaaagaaa aaactaccga gtcggtaatg gtattgtggg
      361 gcagtctaat atctggaacg aaatgatcat gaggcggaaa aaggagaatt aaacgtattt
      421 ttttgagtat attttataga agaataaaaa taaagctcta ttcaagggct ttgttttcca
      481 ggataagcct ttttctctgc caaagtttga aagtcatttc ctgacaaata cactctgggc
      541 tatagatgat tgaaacagtc agatttctga tgctaagtta agatatatac tgcttattct
      601 ataagcggaa atgacattga gtacagcaga agatggttca ggcgcataaa atcctggtta
      661 atggagtaac ccactcctgt agataaatct gccagcggtc ggtgctcaac gttgggcgca
      721 cttcaccaaa gcagctttat tatttccgat agaaaatccc cagacagcct ggggattggt
      781 atggcagatg gtagttttta atctgtatta cctaacaatt ttttaatatt ttctttatat
      841 gtagcgtatt tttcttgttc attacaccac attaaacaag ccttaaaggc ttttttggcc
      901 attttccgat ttccgaccaa taagtagcta atcccagaat aataagatgc cctgggatct
      961 tgcataacta aagttgcaca gcgagcgaaa cacagttgtg catcttcatg atttgataaa
     1021 cgctgatgac acaaccccag agatagggtg tagtcataat tccagtgctc cagatatgtt
     1081 agcagatgaa atagatttct tgcggttata acatcaccag attgatatgc tgtataggca
     1141 taagaatgaa gatcattgag atcttgttgc gtcaaatttg tcagtgatgc taacgtacca
     1201 ccatcctggt agaaatcata gatatcctgt agagagctaa atttcctgct cataaagcct
     1261 catttattat tcgctagatg ctgattttat cggggtcgct ttaatcaaaa agagtcgaac
     1321 aacgctgtgt ttttgttttg ttgtactgga gaataagtta ccaataacag gaatatcact
     1381 taacaacggt ataccatctt tacttgaact ctctttatcc tggataaatc cgccaagcag
     1441 cagactttct ccagcgctta atgtcgcttc tgttgtcatt tctgaatttt gtacttcagg
     1501 taagatgctg ctggcatcct gagcattact ttggttagtc gattgattgc catcctgaat
     1561 atcaagtaac aaacgaactc gttcccgacg ttttccggta agactattcg agttgtcatc
     1621 aagtatacgt ggtgttactc tcagtaatgt tccgctggta atactttcca gagatgccac
     1681 cttttctccg gaaacttttg tataaaacgt tacgttttta tcaagaatcg cctgcatatt
     1741 atttaaggtt ataattgatg gctgagagag tatttttgct ttcgagttct gttgtaacgc
     1801 attcactctg atcatgaaat tattggcgtt gcttatcaca gaagaactga gattagcttg
     1861 cgccgttgag ctgttaaagg caatagaacc ttgccctgca tttaatgtac cggaccagtt
     1921 tactcccagt tgctgaagat catttgcatc aacatcaata attgaaacag aaatctcaat
     1981 ttgcctttgc tcaatatcaa gctcagaaat taattgccga tatatgtcca tagttatttc
     2041 acgatctttt acaacaactg cattaagacg tggatctgcg gaaatcttta cactgttatc
     2101 aaacaggttg ccgtttcttt ctacagcacc tttgcctgtg ctgggaagtg agccattact
     2161 tgccatcgtc ttcaggatgg agacgacgcc aggcacgaca acattttggt cgcggtattt
     2221 atacgttata tctgtcgctg atgcatagtt taatttaaat actctaacga catctttatt
     2281 ttttgcttta gactgtgctt ctttatcaag actttcagat aaagatgtaa tgtattttat
     2341 gcattcagga acgccacgaa cttcaatcga gttaaatgtt gtgatttttc tcacgttaca
     2401 ggagttttta tttactgaga ttgtatcacg cagatatttt aacagtgagt caatatccag
     2461 atatgttggc gtaattatgc ttcttgatat ttcatttgtt ttataaatat acaaaatgtt
     2521 ctcatcataa taccatgtga gatgatagag cctggacagt ttttccagta ctttcattgg
     2581 tttttcattt tttatttcac cagagaaatc atcattcaca gaattactga tgaaaacagg
     2641 gatgctataa ttagcggcaa aatcattgag gattgcacgt accggagagc tttttgtaat
     2701 aataaaatac tcgttcttgc ctaatctttt ttccagagag gacggggcag cctgagcact
     2761 gcagcaaaat agtgctgtaa aaataaaaaa acttattttt ttcattacac aattcgtcct
     2821 atatcagaaa acaaatgtgt tggaggcatc gttgtatctg atgtagatgc ttgctctaac
     2881 ttatcaaatt gcttgtcaag caaagaggcg agtgtgtgtt gcattattgc ttttgacatg
     2941 tttgcgcgat gattattatc tttattaaac agttggcaaa gataccattt tttattagat
     3001 tgaaaaatgc ccatttgttt atattcttta ctaacggcca gaatattaca gagtctgatg
     3061 aaggtatctg ttggaacctg gcgaaaatcg ccattaagct cgatatacag aacccagtaa
     3121 cttccaaagt cgtgtaagaa ggcatttttt tgatagatat gaaaagtaag atgctttggt
     3181 gaacctttga catcttttac aaaatcaaga gccgttagcc agtcactatc attcttaaca
     3241 atgccattat tattgttcat acatattacc cgtcctgtcc tgaggatgac ttgataacga
     3301 cactaatatt ttctagtttg agatcgtcta cgctattctt aactaagttt tttatctgaa
     3361 tgactgatgg tgctaagtta acctctggcg agctaatgac taaaacagcc gctgaggatg
     3421 gttggctctc gttattgtta acatttaatg aaacgctgca atcgataaca ccggggattt
     3481 tacttagtaa cctttctata tcttgttctt tgagatagtt gatttttgca ttttcttgtg
     3541 atggcgatgc cactagctga ctgggcggaa aaataacttc aatatctgca aatttctttt
     3601 tcgggaagcc attgttattt aaaatggtta tcgcacggac aaaatcctct ttttccacac
     3661 ttattgttac atttccactt ttgtccattt ctttagatac attgacatca ttggataaca
     3721 acaatgcttg catctgattg gcttcttttt ccgttaatcc tgtatacaat tgctctttac
     3781 atgcaactgt caaacaaata aaaacaaata aaaaggtgtt tttaatgtat tttctcattt
     3841 tattgtattg aaaccagttt tgtaaccgat gttgaaagaa gacttgctaa cttggatacg
     3901 acatcaacat tgacgtttgc gttgagcaca ttcttttgta atgtgagcac atcttctggc
     3961 gagagtccac ctgcagatag ggcatcgaaa ctgagatcgg tgttcattgt tgcaagatgt
     4021 ctctttttaa tttcttcaat taaaacttgc tctggagaag atacaccagc tgttttagga
     4081 agtaattgat caagaaactg agccgctcca gccgcagagg gcgttggtgc taacggggta
     4141 tggtcatcaa cgaaatttgt ttgggatact ttattaaaat gaatattaat attttctgat
     4201 tgattaatat tgttcatatt tacggttagt ctcttttcat gagagaggta atggcataat
     4261 gcatccatga cctctctatt ttatttatgc gatgcgatga ttaggcatat ttcatcgcta
     4321 atccgccgcc aagcgcagta acaccaccta atacaccggt agcaatacct aaacctaaga
     4381 attcttggga gcttgcatct gttgtgcatg ccattgcgat accagtacct aaagcagcca
     4441 gagctgtagt agcgagtgca attccggctg caattctgat attacgactt accccatttt
     4501 ctggtgtcat tactcctgta ttttcatcca ccgaaacatt tccattgatt gtggctgcca
     4561 gtggtaatac tccgccagaa ggacttaaat ttgctgcatc cattaacttt tctccattgt
     4621 ctaatttaga catttgtctg gttatcaata accaaattat cccatgggca
//
LOCUS       DAZFKM010000232         4605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFKM010000232 DAZFKM010000000
VERSION     DAZFKM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..299)
                     /locus_tag="ACHKAY_004980"
     CDS             complement(<1..299)
                     /locus_tag="ACHKAY_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5703483.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVG"
     gene            complement(299..628)
                     /locus_tag="ACHKAY_004981"
     CDS             complement(299..628)
                     /locus_tag="ACHKAY_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703484.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(625..3237)
                     /locus_tag="ACHKAY_004982"
     CDS             complement(625..3237)
                     /locus_tag="ACHKAY_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5703485.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKV
                     EYQKRLNELAQQAVRFEEQQSSKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAA
                     LAKATSALKNTWSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRTEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            complement(3218..3631)
                     /locus_tag="ACHKAY_004983"
     CDS             complement(3218..3631)
                     /locus_tag="ACHKAY_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5703486.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGIYGGVRYVPASG"
     gene            complement(3658..4080)
                     /locus_tag="ACHKAY_004984"
     CDS             complement(3658..4080)
                     /locus_tag="ACHKAY_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5703487.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHY
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(4094..>4605)
                     /locus_tag="ACHKAY_004985"
     CDS             complement(4094..>4605)
                     /locus_tag="ACHKAY_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703488.1"
                     /translation="FTLAWKPGEEGQKWLIGWFESGDVRAYKIRFPNGTVDVFRGWVS
                     SIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITPVTAIKVTPTSGTVAKGKTTTLTV
                     SFEPESATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEV
                     TVTEAGAAG"
BASE COUNT          916 a         1545 c         1059 g         1085 t
ORIGIN      
        1 cccaccaggc tctgcaggtc ctccgccatc ccggtgacaa gaccgaacag attggacacc
       61 gtcagcgacg ggcgggcact gctgcccttt ccgttcatct caaagccgct gccctcaatc
      121 gggtacgcct gatattcacg cccctgccag gtcaccggct cccctttttc attcagctca
      181 ttgcagaaaa aataccgctc accgccctgc gccgtcaggt cgatttccca gagtaccacc
      241 cgcggtgact gctctgattt aaccgactcg ttcagacttt cttcgtgaat atcctgcatc
      301 agttcaccac ctgcttaaac tccgcgctga actcaacgcg caacatcccg acccgcgcag
      361 accacccggc acaggtcacc tttatctgcc ggtatgcata gggtggcttc cacaaaaatg
      421 ccttccagcc cccgtgctct gccagaaacg cttccagatg ccgggcctcc tcccgggtca
      481 cggaaagcgt cacccggtat gttttcaggt cagcattcag ccctgccgcc atacgctgtg
      541 agtacccgtc accaaaacgc acttcacgca ccgatggctg cgagttcacc tccatatccg
      601 gcttcacttt ccagcgaaag gttttcatcg cccgctccct gataacatac cgccatcacg
      661 caactgcagc cggagctcat cctgcgcccc cttgcgggcc atgtcataca ccgccttcat
      721 cagctgcggc cccgcctgtc cgttggtgcc gtcgttctga atcaccacgt gattgttctg
      781 attaaaatta atgccttccg tccgccgcat ctgcgccgga cttccggcac cgcccacata
      841 accaccttcc gcatagccgc gcatcagacg gtaaagattc cccacaccta tccggctggt
      901 tgcctctttc gtgaaaacaa actccccgcg gtgaactatc cccgcaggct catatttgcc
      961 gcccgtcccc gtaaatcctc cggtcgcgaa atggaagttc gccgccgcag cctcaatggc
     1021 cgtccccgag gaagcagatg caccaccacc gaaagcaccg ccaatggcgc tgccgatacg
     1081 cccgacaatg cccaccatgg cctgtttaag caggatttct gtcatcatgg acagcacaga
     1141 acgggtgaat ccccgccagt ctgcctctgc accggtcagc atcgccgcca tattctgtgc
     1201 aataccgtca aaggtctgcg tggcagcact tttaacctgc gaaaaactgt ccgtcgcact
     1261 ttccgcccac tcgccccagc cggacttcag cccggccatc cagcttccac gaagctgctc
     1321 ctccgcagac caggtgttct tcagtgccga tgtggccttc gccagcgcag ccggattatc
     1381 accgtacacg tcccgaagac gctgcgcttc agactcccgc tgcgcctgac ggtcagtgag
     1441 accgcgggct tttgcgctga tggcggcctg cttcgagctc tgctgctctt caaaccgcac
     1501 cgcctgctgt gccagctcat tcaggcgttt ctggtattca accttgtcgc ccagctcagc
     1561 cagctggcgt ttgtactcca gcgtctcgtc tttatgcgcc agcagggatt tttcctgctc
     1621 agataactgt cgtttcgtgg cagcctcttt caggaccgca tactgacttt ccgccttcca
     1681 taaatcacgg cgctgccggc tgattttctc attcgcaccg ctgtgttttt ccagcgtcct
     1741 gagctcagct tcaagggcaa gcagagcctc tcttgcctgc tcctcttccc tctccccggc
     1801 agagcgcgtt ttcggtgatg tatgcttttt acctgtcagc tcttcagcca gacggctgac
     1861 ggcttcctgc tgccccggac ctttgctgac gcctgttgca cgcgaacggt tgatgtaccc
     1921 catttccccc tggcgtatac gcgcatcccg ttccgcaatg gattttctca gcgccagttc
     1981 atcgcgtttt gttttctcaa taaatacgcg gttctcttct gccagttcac caaacaacgc
     2041 accaacgccg ggcacattct ttgtcgtttc ccaggctgac tgaataaatt cagccagcgc
     2101 cagatccccc tgcacaagca gcagcttcac ttgttcaacg gttccggcca ccacgtcagt
     2161 gatcagactg agtgccccca gtgtatgatc acctatccat gcccatgcgt cagaagtcca
     2221 ggttttaaca tcgtcccaga ttttttccac cggcgtggcc gctttatcaa gttgctccag
     2281 acgtgcattc atgacatccg caaacaggga catcgcctcc gtcaccgcag cctgtttacc
     2341 tttcgtgcgc tcaagctcat caatatggcg taactgggaa acgctcagga agttatactg
     2401 ctgattcagg gaggccagcg ccttcaccgg atctgctgca atcccttcaa aggctttttc
     2461 caccttcccg gcatcgtccc ccaccgtctg cagccatctc tgagaggttt cccccatgat
     2521 ccgtagctgc ccggcggtat atttcccgct ttctgccaga cgggccagat tttctgccgc
     2581 ctgtctgata ccaccaccgg cttcatcgct gatcaccccg gccattttcc acaattctgc
     2641 cgttgtggtg gcagccgccc ctccggtcag gatcagtgaa cgcaataagg cccggtcagc
     2701 ctgctctgcc tgccaggcgg cggcagcaag cgcggccagt acggcaaccc cgccacctgc
     2761 cgccacacgg gccaccgaca taaatcgtcc cagctcaccg gcattccggg cattttcagc
     2821 cagtgcattt gccgtatctg acagcgactc ctctgatgat tcagaggcat ccctgatccc
     2881 gagaagttcc tccttcagca gggtaagcag gctgagcggt ccaccgaatg aatcgctgat
     2941 ctgccccccc tgctgcagca tgataaggaa gggattctga ccaccggcaa gctgagtgac
     3001 aatatccgtg aactgtgcgg gcagtgtgcg catggcagcc ttatactgtc cgactgatat
     3061 cccggctttt tgtgccgcca gcgcctgtcg gctcagcccc tgttcaacag tactggcggt
     3121 ttttctggca tccgcctcca gactggaaaa atgacggcgt acccgggcca tctgttcatc
     3181 aaaccgggcc gcatccagac tcaaatcaat aaccagatca cccgctggct gggacatatc
     3241 tcacacctcc gtaaatcccc gctgaagcca tcattaatgc aacatcatcc tcgctgacat
     3301 ccaccacatc cgcagaaggt gaaatatcgc gccctccgtc ccctccgaac cggacgcctc
     3361 cggcaactcc tgtcgctttc tgcatcagca tttcttcctc gtccggcatc tccgtctgct
     3421 cttcctcaca cgctggagca agcagactga aatccgccgg atgcatatcc ggatcgccaa
     3481 aaaacaggct gagtacggcg tacatcagcc cggaaaaatg agcgtccagt tgggtatcct
     3541 gaaaataatg cgtgcagtaa aaacgtcgcc agtcggcata ttcggtggat gtcatcccgg
     3601 caagcatggc gcgccagtcg ggtctcccca tctctcgcgc cagtttcagg acaaagttca
     3661 gctcgccttc gaatgctttt ttgatgttac cggctcagtc gcttctgctt tcccggtttg
     3721 ttcaggatcg gcatcgtgcc ggttatccag catacctgaa agataaagca cccggttcgt
     3781 tgcctgattc agtgcatcag caggccatcc cagcatcact tcacggcgga tctgctgcat
     3841 ctctgtctcc ggagaggcca gagtgccttt gagggaatgg gaatgccata gcgacatcgc
     3901 cacaagcagg gatgccgttt ccagatatcg ctggttaatg tgcacgacat aatgcttcgt
     3961 tgtctcctgt tgttctgcgt ctgaaacaaa ctttatataa tcaaaccgct gcagcgcaga
     4021 caattcggaa agcgtgacgg acacaccgtt atattcaaat tgttctgttt tcagaaacat
     4081 gtattacctc cgtttaccct gcagcgcccg cttcagtaac ggtgacttca gccactgcgg
     4141 cgaactgacc atttccgctc accacaggga tctgcacctt acctgtcgcc acgccgttta
     4201 ccgtaattgt catatctttc acactaatgg tggctttcga cggatcggcg gaaaccgctc
     4261 tgaacgtctt gtcggttgca ctttccggct caaaagaaac cgtcagggtg gttgttttcc
     4321 cttttgccac cgtaccggat gtcggcgtca ccttaatcgc agtgaccggc gtaattttgc
     4381 tgcgttcttc cgctacagaa ggtttgccca cgttggtcac tttcaccgtg cgggtgatca
     4441 cttctttcgc cgtcacggcc ttaccgatac tgctgaccca gccacggaac acatccaccg
     4501 tgccatttgg gaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     4561 gccatttctg accttcttct cccggtttcc aggccagcgt aaaac
//
LOCUS       DAZFKM010000233         4527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFKM010000233 DAZFKM010000000
VERSION     DAZFKM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..583)
                     /locus_tag="ACHKAY_004986"
                     /pseudo
     CDS             complement(1..583)
                     /locus_tag="ACHKAY_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(543..662)
                     /locus_tag="ACHKAY_004987"
                     /pseudo
     CDS             complement(543..662)
                     /locus_tag="ACHKAY_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(675..2285)
                     /locus_tag="ACHKAY_004988"
     CDS             complement(675..2285)
                     /locus_tag="ACHKAY_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO5703489.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(2960..3949)
                     /locus_tag="ACHKAY_004989"
     CDS             complement(2960..3949)
                     /locus_tag="ACHKAY_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703490.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(4024..>4527)
                     /locus_tag="ACHKAY_004990"
     CDS             complement(4024..>4527)
                     /locus_tag="ACHKAY_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703491.1"
                     /translation="SKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLIP
                     GYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQR
                     IQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESG
                     GNWMDNL"
BASE COUNT         1187 a         1044 c          970 g         1326 t
ORIGIN      
        1 attatttgag attaataaaa catcaccaac ttcaatatca acaagacttc tgtaacatgt
       61 tttactaaat ccaattacaa aatgaatgga ccaatataat gattttttgt ctaacactgg
      121 agcgtttccg tattggcaga ctgtgacttt atctaaccaa agattcaatt cccctgttca
      181 tattcttatt agtggttgag cagggctcaa tactccatca aatactttac ctacgtgcaa
      241 acgattatat tttagttcag gatgctttag atttaacggg gaactggata aactgagcca
      301 ttcccctatt aacctttccg tttctggcat taaagccatc ccagcgagct cagatgaaat
      361 taagggaaat atttctccaa gcaataccca tccagaccag ctgccactct cttctgctat
      421 cggtaaccat ttagcactaa attcgggcat taataatgca acatcttcgc cattttcttt
      481 ccaattttga aaagtggttt caaacgtatg agtgtttcga tttactttcc tcagaccgaa
      541 cattaacttt catccttctg atcgttttcg aatttgcttt cattttcttg ctcctcataa
      601 ggctgatgtc gttgtccttg tcgttcatct tgctcagtca atacccaacg ttgtgctgta
      661 actcattatg aaaactattt aattccctca tcattgataa ggttggacat ctctttcaac
      721 atgagagtca ttgaaccatt cgaccaattt agcggaatga tagggcttgg cgcttcatgc
      781 aaagttcccg acttgtggat atagttatag ctttcgggaa gtgccatttc aggtacactc
      841 ctgccatttg caccaatcat attctcgcct gttatttggg ccaatgatct attcataaat
      901 tgcactgagt cattcaaata ctcttccttt cttgattctt tataaacaat tgctgctgat
      961 tttgcatacc atgagtcaaa aaaccattct gcttctgttg atggaatgaa gctcttttct
     1021 cttttcgcat gtgaattttg atcggtatcg gttttaatat cattgaacca aaaatttgcc
     1081 gactgataat tatctttctc gtatcttttt ataccatacg gacctgctag ttttttaact
     1141 atttttagaa cctgctcttt tcttctggtg tttatctttg caagatttgc aggataaata
     1201 acattaagta aagccgcatc ggcttctctg taatgtttat ctttttccag ataacctggg
     1261 gattctcccc ctaaatcaag ctgtaacgtt attctttcat aacctttatc tatgaggtgg
     1321 ttcaacctcg ttgttgaaag aggttcatcc agttcattgg ctttagcttc gcgtagtaaa
     1381 tcagagacaa aaacagagtc tttcttcgag agtaagttcg acaaacgttc cagtccagag
     1441 gtcaccagtg caacggaaga tgtgtttaat cgggcatctt cttcccatgc tccggaatct
     1501 tccataacat agaaattggc tttatccaga taagcaatca gcagcgcaac cgattttaat
     1561 cggtcacctt tttgccaatc ttcagcgtta attgtatcag tgtcgattgc ttgtataagg
     1621 agatcaagat aaagacctaa cgcatcattt tgtttatggt tccacagctg cggcttgcct
     1681 tcttcctgca catctgccat cacgggggag ttgctgtcaa atcggatatg cacaacattc
     1741 atttgcccgg gaactccatc cagtcgtttg gggttgctaa tgacgtcctg catccgctta
     1801 atctggtcgg gcgtagacat ataatcccac agggtgagca gaaccttttt tgcggctaca
     1861 ctatttccct gatctgatac taatgccata tagccccata aactatccct cagccagaca
     1921 gcatcatagt gggtttctgc cgctgttttg tcatcttcga aagtagaagc aagaataaac
     1981 gttccccagg gtttctccgt caacgttaac tttcgtgtgt tttctgttaa tgttagcaat
     2041 ccttgaattt cgtctttggt atagtggctc tttatttcat tttgtaattt ggctgagtga
     2101 atcaaaagac gataattagg atacttatct ggatttaacg ataaataact tttatctgtt
     2161 gctgcccccg tggaattaag aatgttaaat acagattcac tcgtcacttc tgcccctgtc
     2221 gcctggcatg tgccgcacga aaatatacca gcgagcagaa gagttaatgc agatttccct
     2281 ttcataaaat attccatcaa tcgtttgtta aaagcgtaga taaccactta tttaacaact
     2341 tgaaaataag gtgttttacc tgggttgtta caaaaggatt gcattgcgta aacgcttttt
     2401 atttacaaca aaatggggaa gtattacggc gattgcgaaa agtctgaagc gattgaatta
     2461 agataatata gaaaatcaga tacagcaaaa atggcagatt gatcaggtga agataaattg
     2521 gagcgggcga agggagccta cgacaaatcg aagacaacca gctaacctct tgtttcttaa
     2581 agactgatga atccagttgg ttgtagtgat agtcacacat ttagcgatag acggtcaaga
     2641 cttgaagcac gtaatattcc ctctaaagga atagaactca cggtctgact acccggccct
     2701 tttggtctga ctcagggtcg ggctatgggg acttttggct cgctctttat attagatgag
     2761 gtctcacatt tttcagtcaa aactcatcgt ctaatagccc atagccagac caccagtccc
     2821 agctttagca ggaagaccac gagatagact ttgtgtaggt ccatttggtc ctcctccctt
     2881 ctgcattcac gaactagatt actagtacaa aataaaggcc agacatccag ttcggaagtc
     2941 tgaccaatag tcgttattgt tagttagctt ctttagtgaa gctcaggcgt ttgactacag
     3001 gcaggacagc agtagccgag ccagtcccca tcttgatggt cgtagcagct tcagctacaa
     3061 caaacacctt gccgagaatc tcaaggtcgt gagactggga gtcaccatca cgtgaaagga
     3121 ccacagtaat gtggtcccca tcgtgacgga agcaaccgaa gctcagagcc gtaaaacgct
     3181 tagccatcat gaagacgcca cggtcttcac cgttgtaata ggcgatgtca gtccccaaca
     3241 tatcgacgat agcgtcttca ccaccaaagc cctgcttagc agcaagacgt gacagtgcct
     3301 tgaggtgtga ctcatgggcg atgatgccgt aatcagcaac ctcagaaccg aaagaaggct
     3361 cggtgttctc gtacagcgcg tccatgatag tgtctgcctt agctgtcagg ctaccagtca
     3421 ggaccggaag ctctacagtc ttcaacagct tggaggattc cagtacatcg accatgcgac
     3481 tattgatggt acggaccacg ttatcgcaga tgttcagagc gtcctgctgg agcgtcccct
     3541 gagtgaaacc agtggaagac ttagcgtccc atagcttggt ctggtgggac acgaggatag
     3601 cttgctcaat cggctcaacg gtgatttcag taccagacgg agcggtcacg acttcaggtc
     3661 gcttaccacc acggacagga acgaccacag agcggtcagc cagagtgtga gcaatgattg
     3721 gggtcggtaa gctattgcga gttggcagaa tctcaatcgg gaatggaatg ttatcctgag
     3781 cggacttcag cacaccattc agcatggtct tgacagcttt agccagtgcc agagcataga
     3841 cctgtgggtc agcattggtt ggcagacctt cgttaacctg aatgttgtct gtgtcggtga
     3901 tacgtactgg ctcaatgccg aactgatagc tgtctttagt catagtcata ataatcaatc
     3961 tcctaaatta gtttagatac gtgggtcccg acatcaggtc aggaccactt gtgtgcgtct
     4021 gtcttacagg ttgtccatcc agttaccgcc tgactcaagg tcagcgacga tagcggcacg
     4081 ggagcgacca gcggaatggg tagcactacg aaggttctca ccgtaggtcc ggcgttcagc
     4141 tttgacctgt tcacggcgct gtagctcttt ctgccactct gcatcagtca tgttctcttc
     4201 acgcatgcgc tcggcctgaa tgcgctgctc tttagcgata cgctcagcct cagctcgctt
     4261 gttctgctgg cggcgaccat aggcggtcat caggtcatta ctgaagaagt taccaatggt
     4321 cacgccagct tcagccaggg cttccaataa ctccatagac tcgcggctgg tagacggcag
     4381 gcctgacagg tagcccggaa tcagggtctt gatagcgagg gacagcggat agaactgacc
     4441 gtccagactc acagtattct cgtagagatt gatgtcggtg actgacagtt tccagtcttg
     4501 gttattgaat aacgcactag ctttgct
//
LOCUS       DAZFKM010000234         4514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFKM010000234 DAZFKM010000000
VERSION     DAZFKM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            532..792
                     /locus_tag="ACHKAY_004991"
     CDS             532..792
                     /locus_tag="ACHKAY_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703492.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            789..1337
                     /locus_tag="ACHKAY_004992"
     CDS             789..1337
                     /locus_tag="ACHKAY_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5703493.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1337..1561
                     /locus_tag="ACHKAY_004993"
     CDS             1337..1561
                     /locus_tag="ACHKAY_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703494.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1558..1881
                     /locus_tag="ACHKAY_004994"
     CDS             1558..1881
                     /locus_tag="ACHKAY_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO5703495.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1896..4229
                     /locus_tag="ACHKAY_004995"
     CDS             1896..4229
                     /locus_tag="ACHKAY_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5703496.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1100 a         1046 c         1156 g         1212 t
ORIGIN      
        1 taaagaataa aagatgtctt atgtctgttt aataattaaa cattcaacct ccctgcactc
       61 agttgcattt caggaaaata ttcataaaaa tgaatttgtc tgatgttcat taaacacatc
      121 cggatacatg cggaagtaag atggtaattt aatttcttta atcatcggat ttattgatac
      181 atatggtgag ctgtttattt ttggccgttt tggctctttt attaatcagg tgttgtctta
      241 gtgcttatga tgtaacgccc tgaatgatat gggctggttc agatttgagc tgtgatcctg
      301 cctagtgtaa tagacttttt tattattgat gctccttatt atattccata aaggaattgt
      361 ttgtttttgt tcgtttcata cggtttttag ctgttttatt ttttgagaaa tcaacatttc
      421 acacaaaata aataaagcat gcgcgagccg cttatataaa cataataagg agctacctga
      481 atccgcatgg aattgcccgg actgttgtaa acatattaga gaggtagcca gatgaatagt
      541 atttccgttt ccaccccggc accagctgct ccggtgctcc cggttccggc tcagcaggag
      601 cgcttcatac gattacctga agtgatgcac ctttgcgggc tatcccgttc aaccatctac
      661 gaccttatca gccgcgatgc cttcccgcag cagatatccc tgggtggaaa aaatgtggcc
      721 tgggtacagt cggaggtcag tgcatggatg gcggcgcgaa ttgctgcccg cgagcaagga
      781 ggccatgcgt gaaacttgct ttgcagtctc atcttctttt ttctggcttg cgtcctgata
      841 tcttttccag gtatagtttt cccgctgccg caaaatcggc tgccgggcgt aggaacccga
      901 gttacttatt ggcgacacca gacgcgccgt gcgtcttttt ttacgtcgtt gctcaggcac
      961 acccattttt cgggctgcgg tgcttatacc ttagcccctt tcagataatg gtggtccggg
     1021 cggggcagcc ttcgggctgg ccggtttcca ataaggccgg tattcctacc cccgttcggg
     1081 tcaccaccca tgagcgtagg aactccggtg gtggcattta ccgctactta ttggaggttg
     1141 cccttatggc tacgatcctc gccccgtcac atcctcaatt tatatttgtt tttgccgccg
     1201 ttcgtcgtgc agaacgtaaa ccacgtatca gtatgctgcg tatcatcgcc agcaatgagc
     1261 atcatgcacg tctttcactg gtccatgagt atgtgctttg tcttgctgct cgcctgcctg
     1321 tccgggaggt gatctaatga atcacgccgc aatctcttat gacgatattc tccgcctgaa
     1381 gcatcttcgt aacgtgggag agtttgtcac tggaatggct gttcttcagg attgctacga
     1441 aaaacctgcc agtgctcaat gtgaacaact ggtttccctg atttatctga tgacagagca
     1501 gcttgatggc gtggtacagc gttgccagga tgacctcatg aacatggagg tggtgcaatg
     1561 aaatcacgca ctctttccct ttcacttcgg gtagcgttgt atcgccgcgc ggtagcctgc
     1621 gcctggttga gtgcctgcta tcagcaaaac cgccatctgc agctcactct ggatgaaatt
     1681 gaaaccgcta ttgcccgtga gcttgaagga ttttatctgc gacagcacgg gcaaacaaaa
     1741 ggcatggaaa tcgcctgcgc cctgctttca gacctgatgg aatacggccc tctaatgtcc
     1801 tgtcccgcac tctcccagct cggaatagcc gtaatggatg agttatgcgt ccgtcacatc
     1861 aaaaagccgg ttttacactg agggagaact gcgtcatgtc aggaatgaaa gtcagtcagg
     1921 ccgtgaaggc tgcccgcggt cactgggctc agattctgcc agcgctgggc gtgaatatat
     1981 tgaaaaatcg ccatcagcct tgcccggtct gtggcggtaa agatcgtttt cgcttcgacg
     2041 atcaggaagg acgcggaaca tggttctgca atcagtgcgg agccggggat ggtttagcgc
     2101 ttgtcaccag ggcactgaat gtggatatca gtgaagcagc tgacaggata catggactga
     2161 cacatgatct gtctgtagtt aattccgagg tcaggacgtt aaccgccgat accgatagcg
     2221 ggaaagatgc agcggcagca cttgccgcgc gactgctgca agcctcgcgt gaatctgccg
     2281 gaaacactta tcttacacat aaaggtttcc cggagcatat ttgccatgag ctgacttcag
     2341 ctcataaggc cggtggtatg atgttccgcc ccggtgattt gatcgtccca ctctataacg
     2401 ctgacgggga gctggtgaat attcagctca tcagtggcaa tggtagcaag tgttttctta
     2461 aaggaggtca ggttaaggag gcctatcatc tgatagaagg gggcgggagt tctgtaagaa
     2521 ggatttggat tgcggaaggt tacgccacgg cgcttaccat tcattatttg acaggagaag
     2581 ccgtcatggt ggcattttcg tcggtcaact ttctttctct gagcagcgtt gcccataata
     2641 agtatccggg atatcagtta attattgcag cggaccgcga tctgaatggt tcaggccaga
     2701 acagggctga aactgctgca aaagcgagtc agtgtgacat tgtgctgcca ccggtttttg
     2761 gtgactggaa tgatgcgctt acccaatacg gagaggaatc cacccggaag gcaattcttg
     2821 aggctttgaa gccacataac gccagtcctt tcgacacaat gagtgaagca gaattcacag
     2881 cgatgagtgt cagcgaaaaa gctcagaggg ttcgggagca ttacagggat gcactggcgg
     2941 ttgatccgaa cgggcagctt ttatcccgct acgagtctgg agcgtggaaa gttatttctc
     3001 agtcagattt tgcccgagat gtcgcagccc ttttccagcg cctcggggca ccgttttcct
     3061 caggaaaaat tgcctcactg gtggaaacat taaagttaat tgttccgcag cagcaaaatc
     3121 cgtcacgtca tctgattggt tttcgtaatg gcgttctcga tacgcgaaca gggcttttta
     3181 gtccgcactg taaagagaac tggctgcgta ccctgtgtga agtcgatttc acgccaccgg
     3241 taaaggggga aacgcttgaa actcatgccc cggcattctg gcgttggctg gaccgggccg
     3301 ccggacacaa gccagcaaaa cgcgacatta ttcttgcagc gttatttatg gtgctggcga
     3361 accgctatga ctggcagctc tttctggaag tgactggccc tggtggaagt ggcaaaagta
     3421 ttctggctga aattgcgacc atgcttgctg gagaggataa cgccacctct gcgaccattg
     3481 aaacgctgga gtccccgcga gaacgtgcgg ctctgatagg tttttctctt attcgcctgc
     3541 ctgatcagga gaagtggagc ggcgacggcg ccgggcttaa ggctataact ggtggcgatg
     3601 cagtatctgt ggaccccaaa tatcagaatg cttattcaac acatattccg gcggttatct
     3661 tggccgtaaa taataatcct atgcgcttca ctgatcgcag tggtggtgtg tcccgcagga
     3721 gagttattct gcattttcct gagcagatag caccggaaga acgcgatccg cagttgaaag
     3781 ataaaattgc acgagaactg gctgtgattg ttcgtcagct catgcagcgt ttcagcgatc
     3841 cgatgagcgc cagaacattg cttcagtcgc agcagaattc tgatgaggct ctcaccatca
     3901 agcgtgatgc tgatccagca tttgattttt gtggctacct tgaggcatta cctgacacca
     3961 acggtatgtt tatggggaac gccaatattg tcccacgcca gccacgtaca tacctttacc
     4021 atgcctacct cgtatacatg gaggctaatg gctataaaaa caccctgagt cttacgatgt
     4081 ttggcaaagg gttgccagtc atgctgaagg agtacgggct acattatgag aaacggcgaa
     4141 ctaatcaggg aatgcaaact aacctcactt tgaaagagga gagtaatgca gactggctgc
     4201 ccaagtgtga tgagccaata ttaaaataac gattaactaa cccggctgat gccgggcttc
     4261 ttattggaaa cgcacaaatg tagagatagc tgttcactct tcacctcgct gttaacacgt
     4321 aaacaattga tatataagtt aaaaatggca gggtgaatag tgtgcatggt tttgcgaaaa
     4381 aaaagtattt tttgtagagt caagtcccgg tttggatccg tttaacggaa cctaactaga
     4441 ttgactattt ctctgatgat ttttggataa ataatcaaca aaaatcaaaa tgtgaacaga
     4501 gaatggtggg ggca
//
LOCUS       DAZFKM010000235         4505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFKM010000235 DAZFKM010000000
VERSION     DAZFKM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..269)
                     /gene="ariR"
                     /locus_tag="ACHKAY_004996"
                     /gene_synonym="ymgB"
     CDS             complement(3..269)
                     /gene="ariR"
                     /locus_tag="ACHKAY_004996"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO5703497.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(298..570)
                     /gene="ymgA"
                     /locus_tag="ACHKAY_004997"
     CDS             complement(298..570)
                     /gene="ymgA"
                     /locus_tag="ACHKAY_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO5703498.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(613..849)
                     /gene="ycgZ"
                     /locus_tag="ACHKAY_004998"
     CDS             complement(613..849)
                     /gene="ycgZ"
                     /locus_tag="ACHKAY_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO5703499.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1163..2374
                     /gene="bluF"
                     /locus_tag="ACHKAY_004999"
     CDS             1163..2374
                     /gene="bluF"
                     /locus_tag="ACHKAY_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO5703500.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2579..3310
                     /gene="bluR"
                     /locus_tag="ACHKAY_005000"
     CDS             2579..3310
                     /gene="bluR"
                     /locus_tag="ACHKAY_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO5703501.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3531..3935
                     /gene="ycgX"
                     /locus_tag="ACHKAY_005001"
     CDS             3531..3935
                     /gene="ycgX"
                     /locus_tag="ACHKAY_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO5703502.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3988..4098
                     /locus_tag="ACHKAY_005002"
     CDS             3988..4098
                     /locus_tag="ACHKAY_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703503.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            4334..4378
                     /locus_tag="ACHKAY_005003"
     CDS             4334..4378
                     /locus_tag="ACHKAY_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703504.1"
                     /translation="MFSKLAQSSIKAMF"
BASE COUNT         1351 a          915 c          872 g         1367 t
ORIGIN      
        1 agttacatat catcagctgt gtatcgcaac acgatttcca gtgtttttct aatcacatca
       61 gctttgagaa tatcacttgt tgtttccagg gagtgtatca gacttaagat aatattttta
      121 ttattaacat tatcgcctga aagcattaaa ttggtgacag cctgccctaa cactgctgac
      181 tcttcttcta acaaattacc cgaactgcga aagtaacttg ctaacgcttt atcagttatt
      241 gcattatgaa ttgtagtatc ttcaagcatg gtaacctctc atcttactta tgaaatttta
      301 atgtattctg tttattttct taccattgaa catactttta ttgtcagctg tcgctgttct
      361 tcggtttggc cctcctgaat cactattgct aaaatcacga attatgctgt caattaaatc
      421 gcgtcggaaa gcatcttcct cagccgattt cattacgagt aattgcttaa taagagtctg
      481 caatgaaaaa tttatcttca tgcgcagtat ctggatcacc gcctggccag taatttcata
      541 ttttatacgt tcattatcag atgtcttcat aacgacttca tagctaacct gttaaataat
      601 caggctgttg cattattcaa aaagcaaccc aattagtgca ttataatgtt tctgttcctc
      661 ttccccagtc gcgtgttcca gtcggcaaag aagtttggca caaagcgact ttcgactcag
      721 attacgtcca tctttcaaaa tctcagtcac aatttccccg agagtttcct gctgagtagg
      781 caagttagct tttgcaaagt aacgagtgat tgctcccgca gaatctaaag tcactgaatt
      841 ttgatgcatg ctacgcctct gttaaaaatg ttaatcaatg ttaagtgagg taacaatagc
      901 aaacttgtac gaaatatgtg tacagaatga taaattaatt ttttgcaatt agtgctaatg
      961 catataaaac aatgtgttat gataaaacat attgcatcat ttaactgaca atctatacaa
     1021 gatttttcgc caactagtgt tcaacttttt ccatttttta tcaatgtaaa gaaacggtta
     1081 tgttttggta ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt actctcgcct
     1141 cacccattac cctggattgt taatgcttac cacccttatt tatcgtagcc atatacgtga
     1201 cgacgaacct gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca
     1261 gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg
     1321 tccggaagaa caggttaaaa tgatatatcg ggctatatgc caggatccac ggcactataa
     1381 tattgttgag ctgctgtgcg attacgcgcc tgctcgccgt tttggcaaag cgggaatgga
     1441 attatttgat ttgcgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg
     1501 cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt
     1561 ccttgcaacc gaacaatcaa cctatttcga gatccctgcc gaagactcct ggctttttat
     1621 cgctgacgga tctgataaag aacttgattc ctgtgccctt tcaccaacta taaacgacca
     1681 ctttgccttt catcctattg tcgatccctt atcgcggcgg ataatcgctt ttgaagccat
     1741 tgtgcaaaaa aatgaagata gcccatcagc catagcggtt gggcagcgta aagacgggga
     1801 aatctacaca gcggatctca aaagtaaggc gcttgcattc acgatggcac acgcacttga
     1861 gctcggtgat aaaatgattt caatcaatct attacctatg accctggtta acgaacctga
     1921 cgcagtctct tttttactta atgaaataaa ggccaatgct ctggtgcctg aacaaatcat
     1981 cgttgaattt actgaaagtg aagtcatatc tcggtttgat gagtttgccg aagcgattaa
     2041 atcgctaaag gctgctggta tcagtgtagc aattgatcat tttggcgcag gttttgctgg
     2101 tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac
     2161 caatgttcat aaaagtgggc cacggcaggc aattattcag gcgatcataa aatgctgtac
     2221 atcacttgaa attcaagtca gtgctatggg cgtggcaaca ccagaagagt ggatgtggct
     2281 tgaatccgca ggaattgaga tgtttcaggg agatctgttt gctaaagcta aattgaatgg
     2341 tatcccttcc atagcgtggc cagagaaaaa ataattttca gcacattctt tcacatgatt
     2401 tcagtaaatc atcgtgaaaa tgaatacgtt acgtttaaat ttaatccaca ttaaaaataa
     2461 tgtttcatcc aatttagctg aaatcttatt tatactttgt acaattttag cgtatattgt
     2521 acaaagtatt attgggtcgt gtacaggcga cggagatttg tgaccgcaag gaggaattgt
     2581 ggcttattac agcattggtg atgttgctga acgttgcggg attaatcctg tcactctccg
     2641 ggcctggcaa cgccgctacg gtttgttaaa accacagcgc agtgaaggcg gacaccgact
     2701 ctttgatgaa gaagacatac aacgcatcga agagatcaag cgttggataa gtaatggcgt
     2761 ccctgtaggc aaagttaaag cattactgga aaccaccagc caggatacgg aagatgactg
     2821 gagccgcctg caagaagaga tgatgtcaat tcttcgcatg gctaatcctg ccaaactacg
     2881 cgcgagaatt atttcactgg gtcgagagta cccagtagat caattgatta atcatgttta
     2941 ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc tcccgcatta tgagcagtat
     3001 gtttgacggc gcattaattg aatacgcagc aacctcgctt ttcgaaatgc gccgtaagcc
     3061 cggtaaagaa gccattctga tggcgtggaa tgttgaagag agagcacgat tgtggctgga
     3121 agcatggcgt ttatcattgt caggatggca catttctgtt cttgctgatc ccattgaatc
     3181 gccgcgcccg gaactgttcc cgacgcaaac attgattgtc tggacaggca tggcaccaac
     3241 gagaaggcaa aacgaacttt tacaacattg gggtgagcaa ggttataaag tcatctttca
     3301 tgccccctaa tttcaccttt atcccgcccc ttagacagaa tgttataatc tgaacccaca
     3361 acagcgtttg taccggagct ggtccgttgc aaaatgctca ccgggagaat tcagatattc
     3421 cctctatcac actctgtaca tatcacagcc aaaggacaga ccagattgaa gtggtaaaac
     3481 cgatcgtgca cactacgcag tctcacaact aacaagaggt tattatcctg atggatcagg
     3541 tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga gcaaaacttc tcctggtttt
     3601 attcagaatt aaaacatcac cgtattgcac attacattta ttatctggct acggataaca
     3661 tcagaattat tactcacgat gacacggttt tgttattaag aggaaccagg aacctgttaa
     3721 aagttagtac gaccaagaac cctgctaaaa taaaagaggc cgcattgctt catatttgcg
     3781 gaaaatctac atttcgggaa tactgttcaa cactagcagg cgcgggcgtt ttccggtggg
     3841 ttactgatgt taatcataac aaacgcagtt actacgccat tgataatacg cttttatata
     3901 ttgaagatgt agaaaataat aaaccattaa tctagctaaa gttggatgct taagaaatgc
     3961 ttcataattc agtaaggcat tagcataatg ggaataaaag tacagagact atccctatgg
     4021 atgataaata ctgtcctttt attgctaccc ataaataatc accatgccaa tactatcaaa
     4081 ttgatatttg aaatgtgatc acttgacttt ctatacgtta ttttataacg gttaatatat
     4141 ttataaaaac aacgggtgtg ccatacgccc gtttcaatac ttaacgcaca tgtgttttgg
     4201 tttagtcatc atccagttat atgtatttta gccaggaaca ggttaaacct ttcctatata
     4261 actcaaaaat tgaaacctta ttctcatgtc atgcttatat tcattattat cgttatataa
     4321 aaaggcaacc ataatgttta gcaaattggc acaaagtagc ataaaggcta tgttttaatt
     4381 acaggatgtt cagtcatttg aatgtataac attatagcta aacaaatcta aaacgaagtg
     4441 aataatttat tgttttcact aaatctcatt ttgtttaaca tccattgaga ttccttgctt
     4501 taaat
//
LOCUS       DAZFKM010000236         4316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFKM010000236 DAZFKM010000000
VERSION     DAZFKM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            240..1172
                     /gene="rfaD"
                     /locus_tag="ACHKAY_005004"
     CDS             240..1172
                     /gene="rfaD"
                     /locus_tag="ACHKAY_005004"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO5703505.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1182..2228
                     /gene="rfaF"
                     /locus_tag="ACHKAY_005005"
     CDS             1182..2228
                     /gene="rfaF"
                     /locus_tag="ACHKAY_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO5703506.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2232..3224
                     /gene="rfaC"
                     /locus_tag="ACHKAY_005006"
     CDS             2232..3224
                     /gene="rfaC"
                     /locus_tag="ACHKAY_005006"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO5703507.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3221..>4316
                     /gene="rfaL"
                     /locus_tag="ACHKAY_005007"
     CDS             3221..>4316
                     /gene="rfaL"
                     /locus_tag="ACHKAY_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO5703508.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYY"
BASE COUNT         1144 a          981 c         1049 g         1142 t
ORIGIN      
        1 atattatcca ttcaaatgct tccgttattt atcatacatc ggaatattga tactaaagca
       61 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
      121 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
      181 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
      241 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaaa gccctgaatg
      301 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
      361 acctggtgga tctgaatatc gcagactata tggataagga agacttcctg atccagatta
      421 tggctggcga agagttcggc gatgtcgaag cgattttcca cgaaggcgcg tgctcttcca
      481 ccaccgagtg ggacggcaag tatatgatgg ataacaacta tcaatactcc aaggagctgc
      541 tgcactactg cctggagcgt gaaatcccgt tcctgtacgc ttcttccgca gccacctacg
      601 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aacgtctacg
      661 gttactcaaa attcctgttt gatgaatatg ttcgccaaat cctgccggaa gcgaactcgc
      721 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
      781 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
      841 tcgaaggtag cgagaacttt aaacgcgact tcgtctatgt tggcgatgtg gcagatgtaa
      901 acctgtggtt cctggaaaat ggcgtttccg gcatcttcaa cctcggcacc ggtcgtgcgg
      961 aatccttcca ggctgtagct gatgctacgc tggcttatca caagaaaggc cagatcgaat
     1021 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     1081 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     1141 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     1201 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     1261 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     1321 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     1381 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     1441 tacctaactc cttcaaatcc gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     1501 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     1561 cctggccgct aatggtggaa cgctatatag cgctggccta tgacaaaggc attatgcgca
     1621 cagcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     1681 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggtttttgcc
     1741 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gagctggcaa
     1801 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctcggcgaaa gatcatgaag
     1861 caggcaatga gattcttgcc gctttaaata ccgagcagca ggcatggtgt cggaacctgg
     1921 cgggggaaac acagcttgat caagcggtta tcctgattgc agcctgtaaa gccattgtca
     1981 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     2041 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     2101 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     2161 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     2221 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     2281 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     2341 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     2401 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     2461 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     2521 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     2581 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     2641 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     2701 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     2761 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     2821 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     2881 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     2941 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     3001 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     3061 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     3121 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     3181 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     3241 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     3301 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     3361 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     3421 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     3481 ccctgatgca ggtgctagca tgaaggaaat tttcaaagct attattgaga atactttact
     3541 atgcacaata gcaataccga tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     3601 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     3661 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     3721 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     3781 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     3841 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     3901 acaatggaag ttactattag taggagtaat ggttgccatc attgcattgt cggttatttt
     3961 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     4021 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     4081 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     4141 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     4201 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     4261 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attata
//
LOCUS       DAZFKM010000237         4314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFKM010000237 DAZFKM010000000
VERSION     DAZFKM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..473)
                     /locus_tag="ACHKAY_005008"
     CDS             complement(<1..473)
                     /locus_tag="ACHKAY_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5703509.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLL"
     gene            742..858
                     /locus_tag="ACHKAY_005009"
                     /pseudo
     CDS             742..858
                     /locus_tag="ACHKAY_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(963..1039)
                     /locus_tag="ACHKAY_005010"
     tRNA            complement(963..1039)
                     /locus_tag="ACHKAY_005010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1003..1005),aa:Arg,seq:tct)
     gene            complement(1053..1129)
                     /locus_tag="ACHKAY_005011"
     tRNA            complement(1053..1129)
                     /locus_tag="ACHKAY_005011"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1093..1095),aa:Arg,seq:tcg)
     gene            complement(1137..1212)
                     /locus_tag="ACHKAY_005012"
     tRNA            complement(1137..1212)
                     /locus_tag="ACHKAY_005012"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1177..1179),aa:Ile,seq:cat)
     gene            complement(1241..1954)
                     /locus_tag="ACHKAY_005013"
     CDS             complement(1241..1954)
                     /locus_tag="ACHKAY_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703510.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRALFLMH
                     YGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNNPALAEALITSQRFSGD
                     TTDMFLMMACLSLFESDERILLFLSGCLSSISAKVRAIIQTDISASWTLGAIALRLHM
                     SESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(2092..2289)
                     /locus_tag="ACHKAY_005014"
     CDS             complement(2092..2289)
                     /locus_tag="ACHKAY_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5703511.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(2576..3394)
                     /locus_tag="ACHKAY_005015"
     CDS             complement(2576..3394)
                     /locus_tag="ACHKAY_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO5703512.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(3546..3917)
                     /locus_tag="ACHKAY_005016"
     CDS             complement(3546..3917)
                     /locus_tag="ACHKAY_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5703513.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(3907..4278)
                     /locus_tag="ACHKAY_005017"
     CDS             complement(3907..4278)
                     /locus_tag="ACHKAY_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5703514.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
BASE COUNT         1312 a         1098 c          857 g         1047 t
ORIGIN      
        1 agcagttttt tggcaatatg taagccctgg ccgacacagc cgtactgccc tttgctcagg
       61 tctgccttcg gatgattcag cgtactcata tcctgcacat catgcaggca gtggtcagcc
      121 ggaatgatgt cgttatacgt acaactctct ccacccggag ttaccgtgct gcggcgcgcc
      181 agctgtttaa tgcgcggatc cggagcatcg taagaatccg gtaacggaag cccttcaccg
      241 taggccatgc cgttggactg tccggcaagc acaaccacgt agaaccagtc cggctcagat
      301 gaagggccga cctgtggatc tccttcaata gccaccgcct gcatcagtgt gtacggcgta
      361 atggcaaccg gtccgccgta tggctgccag ccctctttca gtttgtgtgt cagcttttcc
      421 gcaaggtctg acggcgacgc cgccctgaca acatcatagt gtttaaatgc catgaatcct
      481 cccggccggg ataatattgt gagtaaaatg aggagcgggc tgaagtccgg aagttacagg
      541 acaatggcag aagagagaca acagcccgca atacgaaaaa ggccgcgcta ttgcgcagag
      601 tgattactgt cggatattat tcgccagctg aaatattact tcacgttttg ttgtttattc
      661 cttgccgccc gcgtctccct gcgcgggctt tttttgtcca taagaaagcc cctccggaga
      721 ggggctggag agtggcgcta tgtgccattg catggtgccg ggtgcctccc ggtgaattca
      781 gtaccagcac ctgaatccgc gattatccca tatacctact cgctgattgc ccctccgcac
      841 agggggattc accatgccag tttcttttaa caaactcccc gcaaaccaga caacagtcaa
      901 ccgcctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa agcgagccag ggaaaataag
      961 tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc ttagaaggca attgctctgt
     1021 ccggctgagc taacaacgca ggatacagat aatggaccgc cttcggggac ccgaactccg
     1081 cgcaaccagc ttcgaaagct ggcgctcttt cctgatgagc taatggcggt atgtgatggt
     1141 ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct ctcaccactg
     1201 agctaaaggg ccgggtcaaa aaataataat cagatgaaaa tcaataatca agcccttgcc
     1261 tggatacata tctgtctggc gggaagccat aatagcggtg aaatacagaa ataaagtagg
     1321 accagcttga ataaccgcat ttttctgcta cagcctgtcc atatccatgc cgggaacata
     1381 acatattcac agcaacacgc atccgctctt ccagcaacaa gcgactgaac atgtgccctt
     1441 catttttcag ttttatcttt aacaaactct cactcatatg caggcgtaac gcaatcgcac
     1501 caagcgtcca gcttgctgat atatctgtct gaattatcgc cctgactttg gcacttatac
     1561 tggataaaca tccacttaaa aataataata tccgttcatc tgattcaaac agcgacaggc
     1621 atgccatcat aagaaacata tccgtggtat ctccggaaaa tctctggctg gtaattaaag
     1681 cctcagccaa cgcagggttg ttgggttcca gtgacagata aagcggaacg tcagtcagat
     1741 gagttcttgt cagcttatgc tgaatttcca gatattgact tactatggaa tggtttatat
     1801 cgaaaatttt aactttgcca taatgcataa ggaaaagcgc cctgatgcat ttggtggcca
     1861 gaacgactga gccgggctta agtgacaacg tatccttttc aagaaaaata ttaattgggg
     1921 agcaaaccat gataactgaa cagacaacaa ccattataat tttactttca ttagcaattg
     1981 gttagttcaa ttatagcccc aaaaggtaaa ttatcatcaa cacataagca aaggactgac
     2041 aggtgccgct aacacccacc agccgcccat ttaccacaaa taaaaaagcc ttcaggactg
     2101 aaggcgtctg taacaaccgc actgatagtc tgccagaccc gccataacaa gctgggtcag
     2161 tattaactgg cagcgttcgc gtgaaaggta agtattctgc gcaatctccc cgactgtcgc
     2221 cggttcggtg acgcttaatt catcaaacac aactctggcg gtttctgtca tatcctgctg
     2281 tttcagcatg tctttttacc ctttccggtt aacgtgacac accaataact cttgccgaaa
     2341 aagccagcaa gctgaaagac ctgtattaat aactaccagc acatttaacg cactgcgcta
     2401 ctttgcgggc acaaaaaacc cgctcagagg cggggtcaag ctatgcggcg aaataaccac
     2461 tcttaacagc atacctgatt ttttacgtac gtaaatgctt tgccgtgcat atttttcatg
     2521 caaatgtcac gtcctgctat ttttcagtct tataaattta aaaccataga aaaaatcaat
     2581 tatgttttaa aaatggatag gtaaagaata acaagtgaca cagattcaaa ccaaaatgga
     2641 aaagggtggc aacccacaaa cgcccactcc acatccatga caatccatac acaacaccag
     2701 ataacgtggc aaataataca agtaaagcac cacctgaata gtgataaaaa ccaaacaaca
     2761 aagccgccac aattaatgca accaatggag acgttacttc tgatagccgt gattgaatat
     2821 accctctaaa taatgattcc tctgccagag acacaaaaaa caaattagcc aatataaact
     2881 ctggcaacca ctcaggaaaa tgaatctctg gctttaatcc accaaaaaaa acagccaaaa
     2941 acaggataag aggaacagag agcgacagag ccccccactt ccacaaagac acttctgatt
     3001 ttacttcttt tttaaacaaa gaagatgtac acaggactaa caaaaatggc accagcgctt
     3061 tatcaaaatt aaaatacatt gtatagggag tactctgagg gccaacagta acagaattta
     3121 gcactacagg attgtgaaac cctggccata gatggaaaga taatgctatg gctgataaaa
     3181 ctatgccaac ttcatatata gatttagccc aggcgttata tttccagttg aacttcaaaa
     3241 taataaaaaa aacgattgta gcaacaaaaa ataacaccga ccaatcaata atatcattaa
     3301 gcacagccag gacaacagac accgtcaaca atgaaaaagc cactacctta tgccaactga
     3361 gagttgacag tgacagtact aacacaatcc acataagcac tccttttatt taatgatgaa
     3421 gattgattat caatattttc aattcaccag gcaacatttt atctaccttc cacaatacat
     3481 gaccatcaaa aaataaacat gttaattata aacacagaaa acataaccct catcactata
     3541 tatccctacc gcatatccat atctaaccgg acattcagag ccataagcat tccttcaata
     3601 ataccttccg ctttataaag ccttttacca ataagcccat cagaacatct atgcttacgt
     3661 gcaagagcca taaatgtcat tccacctacg taataatcca ccaacaaatc gtgcaaatac
     3721 tgattattct tgtttaatcg tgccatacaa ccacaaatta tcatggcgtc atcatcagaa
     3781 cactgaggac gtgatttcac tttagtcggg attaatcctt taaaaccagc agctatcgag
     3841 ggccatgtta catcttcatg attatttgct gcccatgccc cccatcgctc aagaaccatc
     3901 tggatatcac gcgccatcgt taccacctct gatttcgtaa atcttcacgc ccagccgtcc
     3961 acctggcaca ggctgaccgc gcacaatatt gatttcatca aactgctcgt catcaatgag
     4021 cacttccgca tgcgtcagcg catccagcgg tgctttcaga atgttgtcca ggtcacggcg
     4081 gcgcttatcc ggtggttctg caataatttt tattgccagc cgtccggaca ggcttaattt
     4141 cagccgctgc tggcgaacaa taagcgccac agcccggcga taacgctcac cggcttttga
     4201 tacaaaatat gtgctgccac gacgtcgcca gtaggtgttc accgtcggcg ggtaaggcaa
     4261 aacaaactct atacgcatca gtaacctctt ttacccgagc acgccggttg caaa
//
LOCUS       DAZFKM010000238         4301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFKM010000238 DAZFKM010000000
VERSION     DAZFKM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            381..989
                     /locus_tag="ACHKAY_005018"
     CDS             381..989
                     /locus_tag="ACHKAY_005018"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO5703515.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1087..2478
                     /gene="selA"
                     /locus_tag="ACHKAY_005019"
     CDS             1087..2478
                     /gene="selA"
                     /locus_tag="ACHKAY_005019"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO5703516.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2475..>4301
                     /gene="selB"
                     /locus_tag="ACHKAY_005020"
     CDS             2475..>4301
                     /gene="selB"
                     /locus_tag="ACHKAY_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO5703517.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT          959 a         1103 c         1264 g          975 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt
      181 tgtctgcttc caggcaaaac cctacagatt tataagaaag ccttaagtga gatatccatc
      241 agatagaaac aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa
      301 gtaacaaacc atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt
      361 cctcgcacaa aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca
      421 aactttctat tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct
      481 ataacgcgga caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga
      541 ccgaagaggg cgaatgttgg tttgattcgc cgatcatcgc tgaatatatt gaattaatga
      601 atgtcgctcc ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg
      661 aggcactggc ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc
      721 cagcggcgca gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc
      781 tggatgtgct ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg
      841 cgactatcgc cattgcctgt gccgtcggat atctcaattt ccgccgcgtt gcgccaggct
      901 ggtgtgtcga tcgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt
      961 ttgctcgcac cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg
     1021 ttacaatccg tggttatgtt aaacgccctt ctccgtgtga gagggccttg atcagccagg
     1081 tttcctatga caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta
     1141 ttgcgcgata gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa
     1201 ttgttgcgtc agatgctcga cgaagcgcga gaagtgattc gtggcagcca gacgctgcct
     1261 gcgtggtgtg aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga agcgcagagc
     1321 gcgctgcgtc cggtgatcaa cctgacggga accgtgctgc ataccaacct tgggcgagct
     1381 ttacaggcgg aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc
     1441 gagtacgatc tggacgacgc tggacgcgga catcgcgatc gtgcgctggc gcagttgctg
     1501 tgccgcatta cgggggcgga agatgcctgt atcgttaata acaatgcggc ggcggtgtta
     1561 ttgatgttgg cggccactgc cagcgggaaa gaggtggtgg tttctcgcgg cgaactggtg
     1621 gagattggcg gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac
     1681 gaagtaggga ccaccaaccg cacgcacgcg aatgattatt gtcaggcggt gaatgaaaat
     1741 accgcactgt tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg
     1801 atagatgaag cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat
     1861 ttaggcagtg gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg
     1921 caggagttga ttgcggcggg cgtcagtctg gtgagtttct ccggcgacaa gttgttaggc
     1981 gggccgcagg caggaattat tgttggtaaa aaagagatga tcgcccgcct gcaaagccac
     2041 ccgctgaagc gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg
     2101 cgtctttatt tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc
     2161 cgcagcgcag aggtcattca aatccaggca caacgtttac aggcccccct tgccgcacat
     2221 tacggcgcgg agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg
     2281 ctgccggttg atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt
     2341 agccaccttg agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt
     2401 atttatgacg gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg
     2461 gagatgttgt tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct
     2521 tattgcaggc gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca
     2581 tgaccatcga tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctggtttta
     2641 tcgacgttcc cggtcatgaa aaatttcttt ccaacatgct ggcgggcgtt ggtggtatcg
     2701 atcacgcgct gttggtggtg gcgtgcgatg acggtgtgat ggcacagacc cgtgagcatc
     2761 tggcgatttt gcagctgacc ggtaacccga tgctgacagt ggtgctgacc aaagccgatc
     2821 gcgtggacga agcgcgtgtt gatgaggttg aacgtcaggt aaaggaggtg ctgcgggaat
     2881 acggttttgc tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcga ggaattgatg
     2941 ccctgcgcga gcatctgctt cagttaccag aacgtgaaca cgccagccaa catagtttcc
     3001 gcctcgcgat cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg
     3061 cgttaagcgg ggaagtgaag gtaggcgatt cactatggct aactggtgta aataaaccga
     3121 tgcgtgtacg tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc
     3181 gtatcgcgct taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc
     3241 tgcttgccga tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata
     3301 cgccgctgac ccagtggcag ccgctgcata ttcaccacgc cgccagccac gttacgggac
     3361 gcgtttcgct gctggaagat aaccttgccg agctggtgtt cgacacgccg ctgtggctgg
     3421 cggataacga tcgcttagtg ctgcgcgata tctcggcccg caacacgctg gcgggggcgc
     3481 gtgtggtgat gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat ctgcaatggc
     3541 tggcgtctct tgcacgggcg cagagcgatg ccgatgcgtt atctgttcat ctggaacgcg
     3601 gcgcggttaa ccttgcggat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg
     3661 aattgctgca acagcctggt tatattcagg ctggttatag cttgttgaat gcgccggttg
     3721 ccgcccgctg gcagcggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg
     3781 atgaacctgg tcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag
     3841 cgctggtact gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg
     3901 gttggctgca tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc
     3961 aaaaagcaga gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga
     4021 cgggtaccga cgagcaggca atgcgcctga cgctacgcca ggcggcgcag caaggaataa
     4081 ttaccgcgat cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata
     4141 tgatccgcga tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct
     4201 taggcgtagg ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta
     4261 cgcgtcgtcg tggaaatgat catttattgc gcgacgcatt a
//
LOCUS       DAZFKM010000239         4290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFKM010000239 DAZFKM010000000
VERSION     DAZFKM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..798
                     /gene="ydeO"
                     /locus_tag="ACHKAY_005021"
     CDS             37..798
                     /gene="ydeO"
                     /locus_tag="ACHKAY_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO5703518.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            1200..2882
                     /gene="ydeN"
                     /locus_tag="ACHKAY_005022"
     CDS             1200..2882
                     /gene="ydeN"
                     /locus_tag="ACHKAY_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5703519.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKQKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2934..4091
                     /gene="ydeM"
                     /locus_tag="ACHKAY_005023"
     CDS             2934..4091
                     /gene="ydeM"
                     /locus_tag="ACHKAY_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO5703520.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4057..4179)
                     /locus_tag="ACHKAY_005024"
     CDS             complement(4057..4179)
                     /locus_tag="ACHKAY_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703521.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT         1313 a          893 c          844 g         1240 t
ORIGIN      
        1 gtatcgataa aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata
       61 tttattcatc atgccttcaa cgctaacatt ttagataaag attacgcctt ctctgacggc
      121 gagatcctga tggtagataa cgctgttcgt acgcattttg aaccttatga gcggcatttt
      181 aaagagatcg gatttactga aaataccatt aaaaaatatc tacaatgcac taacatccag
      241 acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg
      301 cttaatgaaa tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa
      361 cttttgcttt tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta
      421 actaacggtg tgctatccgt ttccggaaaa gtgagaaata ttgtcaacat gaagctggcg
      481 cacccatgga agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag
      541 aaaaaactaa agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag
      601 cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt
      661 tatgccagta catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag
      721 agagtttcta aggagtaccg ttgtcaaagt cacacgggta tgaatacggg caacacgatg
      781 agtgctttag ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa
      841 ttatatgggg cagccattcc ctgccccgcg ttgtttttag gcgatatatt tattgaaata
      901 aataagtgac atccatcaca tatttatgca cttgcataac ctgttgcatg attatttatg
      961 atctcaattc ggcattttgt cagtaaaatg caataattta ttaaatatca ataaattagt
     1021 tgtttatcgg cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg
     1081 ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc
     1141 cacttatttc tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta
     1201 tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg
     1261 gtatggctgc atttgctgct catgcggcag atgatgtaaa gcagaaagca accaaaacaa
     1321 acgttgcttt ctcagacttt acgccgactg aatacagtac caaaggaaag ccaaatatta
     1381 tcgtactaac catggatgat cttggttatg gacaacttcc ttttgataag ggatcttttg
     1441 acccaaaaac aatggaaaat cgtgaagttg tcgataccta caaaataggg atagataaag
     1501 ccattgaagc tgcacaaaaa tcaacgccga cgctcctttc attaatggat gaaggcgtac
     1561 ggtttactaa cggctatgtg gcacacggtg tttccggccc ctcccgtgcc gcaataatga
     1621 ccggtcgagc tcccgcccgc tttggtgtct attccaatac cgatgctcag gatggtattc
     1681 cgctaacaga aactttcttg cctgaattat tccagaatca tggttattac actgcagcag
     1741 taggtaaatg gcacttgtca aaaatcagta atgtgccggt accggaagat aaacaaacgc
     1801 gtgactatca tgacaacttc accacatttt ctgcggaaga atggcaacct caaaaccgtg
     1861 gctttgatta ctttatggga ttccacgctg caggaacggc atattacaac tccccttcac
     1921 tgttcaaaaa tcgtgaacgt gtccccgcaa aaggttatat cagcgatcag ttaaccgatg
     1981 aggcaattgg cgttgttgat cgtgccaaaa cacttgacca gccttttatg ctttacctgg
     2041 cttataatgc tccgcacctg ccaaatgata atcctgcacc ggatcaatat cagaagcaat
     2101 ttaataccgg tagtcaaaca gcagataact actacgcttc cgtttattct gttgatcagg
     2161 gtgtaaaacg cattctcgaa caactgaaga aaaacggaca gtatgacaat acaattattc
     2221 tctttacctc cgataatggt gcggttatcg atggtcctct gccgctgaac ggggcgcaaa
     2281 aaggctataa gagtcagacc tatcctggcg gtactcacac cccaatgttt atgtggtgga
     2341 aaggaaaact tcaacccggt aattatgaca agctgatttc cgcaatggat ttctacccga
     2401 cagctcttga tgcagccgat atcagcattc caaaagacct taagctggat ggcgtttcct
     2461 tgctgccctg gttgcaagat aagaaacaag gcgagccaca taaaaatctg acctggataa
     2521 cctcttattc tcactggttt gacgaggaaa atattccatt ctgggataat taccacaaat
     2581 ttgttcgcca tcagtcagac gattacccgc ataaccccaa cactgaggac ttaagccaat
     2641 tctcttatac ggtgagaaat aacgattatt cgcttgtcta tacagtagaa aacaatcagt
     2701 taggtctcta caaactgacg gatctacagc aaaaagataa ccttgccgcc gccaatccgc
     2761 aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat cgacagcagc cagccaccgc
     2821 ttagcgaagt aaatcaggag aagtttaaca atatcaagaa agcactaagc gaagcgaaat
     2881 aactaaacct tcatgcggcg gaattttccg ccgccttatt gagcgagata gcgatgcacg
     2941 ttacagccaa gccctccagt tttcaatgta atctcaaatg tgattactgt ttttaccttg
     3001 aaaaagagtc gcagtttact catgaaaaat ggatggatga cagcactctg aaagagttca
     3061 tcaaacaata tatcgcagcg tctggcaatc aggtctattt tacctggcaa ggcggtgaac
     3121 ccactctggc tggcctggat tttttccgta aagttattca ctatcaacaa cgctatgcag
     3181 gccaaaaacg tatttttaat gcattacaaa cgaatggcat tttattgaat aatgaatggt
     3241 gtgcttttct caaagaacat gagtttctgg ttggtatctc gatcgatggc ccccaggagc
     3301 tacatgaccg ttacagacgc agtaattcag gtaacggtac ttttgcaaaa gtgatagcag
     3361 ccatcgagcg tctgaaatca tatcaagttg agtttaatac gttaaccgtc attaataacg
     3421 ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata
     3481 tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt ggtcatagtg
     3541 agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt
     3601 caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatt cgtcagtttg
     3661 aaagctttgt cagccgtttt ttggggaatg ggcataccag ttgtattttc caggagtcct
     3721 gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct
     3781 atccacagta caaaattgga aacattaata aatctgaact caaaacgatg aacagtgtac
     3841 aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     3901 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     3961 atttttgcga aggttataaa aacctttttt caaccatggt accttatatg aacgccatgg
     4021 tggagttagc taagaacaga gtaccgcttt atcacattat ggatgttgca aaacaaatgg
     4081 agaataatta ataacacgcc cttttagctc cattcttagc ctccttctta tgaccttaac
     4141 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     4201 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     4261 ccataactaa tgaaaatgaa atatttcttt
//
LOCUS       DAZFKM010000240         4284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFKM010000240 DAZFKM010000000
VERSION     DAZFKM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHKAY_005025"
     CDS             <1..75
                     /locus_tag="ACHKAY_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO5703522.1"
                     /translation="RMAEHIRYMVETIAHHQVDIDSEV"
     gene            81..866
                     /gene="bet"
                     /locus_tag="ACHKAY_005026"
     CDS             81..866
                     /gene="bet"
                     /locus_tag="ACHKAY_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO5703523.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            863..1540
                     /locus_tag="ACHKAY_005027"
     CDS             863..1540
                     /locus_tag="ACHKAY_005027"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO5703524.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            1540..1722
                     /locus_tag="ACHKAY_005028"
     CDS             1540..1722
                     /locus_tag="ACHKAY_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO5703525.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            1695..1886
                     /locus_tag="ACHKAY_005029"
     CDS             1695..1886
                     /locus_tag="ACHKAY_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO5703526.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            1963..2178
                     /locus_tag="ACHKAY_005030"
     CDS             1963..2178
                     /locus_tag="ACHKAY_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO5703527.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2277..2498
                     /locus_tag="ACHKAY_005031"
     CDS             2277..2498
                     /locus_tag="ACHKAY_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO5703528.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2495..2800
                     /locus_tag="ACHKAY_005032"
                     /pseudo
     CDS             2495..2800
                     /locus_tag="ACHKAY_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            3097..3447
                     /locus_tag="ACHKAY_005033"
     CDS             3097..3447
                     /locus_tag="ACHKAY_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5703529.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            3522..3764
                     /locus_tag="ACHKAY_005034"
     CDS             3522..3764
                     /locus_tag="ACHKAY_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5703530.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            3883..4227
                     /locus_tag="ACHKAY_005035"
     CDS             3883..4227
                     /locus_tag="ACHKAY_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703531.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT         1224 a          978 c         1115 g          967 t
ORIGIN      
        1 cgcatggcag aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt
       61 gattcagagg tataaaacgg atgagtacag cactcgcaac gctggctggg aagctggctg
      121 aacgtgtcgg catggattct gtcgacccac aggaactgat caccactctt cgccagacgg
      181 catttaaagg tgatgccagc gatgcgcagt tcatcgcatt gctgatcgtc gccaaccagt
      241 acggccttaa tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcatcg
      301 ttccggtggt gggcgttgat ggctggtccc gcatcatcaa cgaaaaccag cagtttgatg
      361 gcatggactt tgagcaggac aatgaatcct gcacatgccg gatttaccgc aaggaccgta
      421 atcatccgat ctgcgttacc gagtggatgg atgaatgccg ccgcgaacca ttcaaaaccc
      481 gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacgtcata
      541 aagccatgat tcagtgtgcc cgtctcgcct tcggatttgc tggtatctat gacaaggatg
      601 aagccgagcg cattgtcgaa aataccgcat acactgcaga acgtcagccg gaacgcgaca
      661 tcactccggt taacgatgaa accatgcagg aaattaacac tctgctgatc gccctggata
      721 aaacatggga tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgtg
      781 catcgtcaga actgacacag gccgaagcag taaaagctct tggattcctg aaacaaaaag
      841 ccactgaaca gaaggtggca gcatgacacc ggacattatc ctgcagcgta ctgggatcga
      901 cgtgagagct gtcgaacagg gagatgatgc gtggcacaaa ttacggctcg gcgtcatcac
      961 agcttcagaa attcacaacg taatagcaaa accccgatca ggaaagaagt ggcctgacat
     1021 gaaaatgtcc tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt
     1081 taacgctaag gctctggcct ggggaaaaca gtacgagaac gacgccagaa ccctctttga
     1141 gttcacttcc ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg
     1201 caccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgagc tgaaatgccc
     1261 gtttacctcc cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaaatcggc
     1321 ttacatggcc caggtgcagt acagcatgtg ggtgacacga aaagatgcct ggtactttgc
     1381 caactatgac ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga
     1441 aaagtacatg gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc
     1501 actggctgaa attggttttg tatatgggga gcaatggtaa tgaagcatcc tcacgataat
     1561 atccgggtag gcacgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc
     1621 ggcctttctg ttatccgaaa tccactgaaa gcacagcggc tggctgaaga gataaataat
     1681 aaacgagggg ctgtatgcac aaagcatctc ctgttgaatt aagaacgagt atcgggatgg
     1741 cacatagcct cgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag
     1801 aatttcatac gttagccaca tccctttcac aaaagctgga aatgatggcg gcgaaagcag
     1861 aagcaaacga gagagacccg gcatgacaac aacagaatgc atttttctgg cagcaggctt
     1921 catattctgt gtgcttatgc ttgccgacat gggacttgtt caatgacacc tcagcaggaa
     1981 aacgcccttc gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcag
     2041 tttccggata aaaacgtcga tgacatttgc cgtagcgtac tgaagaagca ccgcgaaacg
     2101 gtaacgctaa tgggattcac accgactcat ttaagtctgg cgatcggcat gttaaacggc
     2161 gtctttaagg agcgatgaac atgaaaagca aaatcatcag ggagctacag gctccttttt
     2221 tattgttcgc attcaccctc aagcgtatta accaacaatt cagggattaa tggaagatgg
     2281 cagacatcat tgattcagca tcagaaattg aagaattaca gcgcaacaca gcaataaaaa
     2341 tgcaccgcct gaaccaccag gctatatctg ccactcattg ttgtgagtgt ggcgatccga
     2401 tagatgaact aagacgtctg gccgttcagg gttgtcggac ttgtgcaagt tgccaagagg
     2461 atctggagct tatcagtaaa cagagaggtt cgaagtgagc gaaattaact ctcaggcact
     2521 gcgtgaagcg gcagagcagg caatgcatga cgactgggga tttgatgcgg accttttcca
     2581 tgagctggta acaccatcga ttgtgctggc actgctggat gaacgggaaa gaaaccagca
     2641 atacatcaaa cgccgcgacc aggagaacga ggaaattgcg ctaacggtag ggaagctgcg
     2701 tgttgagctt gaggaagcaa aatcaaaact caacgagcag cgtgagtatt acgaaggtgt
     2761 tatctcggat gggagtaaac gtattgctga actggagaaa agcgaagagc aactcattaa
     2821 cgagcgtgac catgctgagt ctgctttagc tgatatgtac ttcgcaacaa ccggggatag
     2881 gcctgagtgg agtaactgtt tcagtttttc agatgctgtc gatgccgtag ttgacagaat
     2941 tgctgattta gaagccaaac agccatcgcc agtagtaccg gaagaaaaac caatgcctaa
     3001 ccctcttagc atgtacacag ttgatgctgt tgcagctatt gcagaggtga gaggctggaa
     3061 cgcctgccgt gcagccatgc ttaagggaga taaatcatga ttaatcgaac caaactggag
     3121 cacatcctcg agtatgccag gcagcagagg cgctttggcc agctttgtaa aattctgcca
     3181 ggagatatgg ttgaaatcgt ggagattgcc atgcgtaaga ctggcaactc tccggtaagt
     3241 ccgggtggtt ggataagctg tagtgagcga atgcccgctc aagatgattg gattttaatt
     3301 tattcaaagc acggtgagta tatggcagga caggtacaag aggaatacgt ggagttgagc
     3361 gacggcactt tatcgtggtt agggaacgcc ttgtactgga tgctgctacc agaaccgccg
     3421 cagggagtga atgatgaatt ggcctgaagc attcaccgct gtaggagttg caatcgcggt
     3481 ggcatttatt ctgtattcgc ttttccgctg gggataaagg aatgttcgct ctgattcaac
     3541 gtggtcagat atacaccgat agcgccggct acccgataaa aattcttcgc tgcataaaca
     3601 acactgtgtt gtacagaaga atggatgggc gaacacagtc ggtaaaaata aacgatttta
     3661 atgaactgtt tgaacggatc gatcaccagg aataccgaca aattctggct gaaacagagc
     3721 aggagaacca tctgaaaaaa ttacgcgcca tgcaaaggag ataaaccggt aaaggtgttc
     3781 gcgataaagg tgaatatcgg caatgaataa caatcctcgc actcgcgggg atttctttta
     3841 tctgaactcg ctacggcggg ttttgtttta tggagacaag aaatgtcaga tttggctatg
     3901 aaggttttga aatggcaatc gactggcgat gttggcatca gtagcgcaac tcttgcctca
     3961 atcgcatgtg gactgaaaaa gaatatctat ggtcatcact tcggcgctcc acatgacgca
     4021 gcagacttcc ggcgatgcgt tgcacttgtt gagcagattc cagaaatcag agattcattc
     4081 gacaaggttg caaagcgcgt tccggcattc aaaggcatcc tcaacgaatg ggattccctc
     4141 gtcgctctgt tgaagtctga aatgaagata cacggaaaca aagcaccaga gacttacaga
     4201 agaattagcg agctacgcaa ggactaacca cagcctcaca ctcaatgagg cctgttcatt
     4261 tctcaagata tccagaccta ccat
//
LOCUS       DAZFKM010000241         4284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFKM010000241 DAZFKM010000000
VERSION     DAZFKM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..268
                     /locus_tag="ACHKAY_005036"
     CDS             <1..268
                     /locus_tag="ACHKAY_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system, LEE associated
                     protein"
                     /protein_id="HFO5703532.1"
                     /translation="DFNGFSFFIEIIDSNVFITFALEYNENAFFSFFNALAPERTQGV
                     IEHIFVYDNKLCLSCLLTNIDVFSLMNTFQQHVQIIERVRRMTS"
     gene            265..2292
                     /gene="escV"
                     /locus_tag="ACHKAY_005037"
     CDS             265..2292
                     /gene="escV"
                     /locus_tag="ACHKAY_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO5703533.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLTDDTVYFEAGHAEISFEFVQEKLSANSIVYKTNEAN
                     QQLAHVSGMDVYAKTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVRGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIVNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
     gene            2276..3616
                     /gene="escN"
                     /locus_tag="ACHKAY_005038"
     CDS             2276..3616
                     /gene="escN"
                     /locus_tag="ACHKAY_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO5703534.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            3676..3996
                     /locus_tag="ACHKAY_005039"
     CDS             3676..3996
                     /locus_tag="ACHKAY_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO5703535.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQKRTKEDLQHVNKSIEKLSFAIKE
                     HYFD"
     gene            3989..>4284
                     /locus_tag="ACHKAY_005040"
     CDS             3989..>4284
                     /locus_tag="ACHKAY_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFO5703536.1"
                     /translation="MTSISLKRNLIEPVSFQPSDDWPQGEESFNDIFNRIKKRTPDTS
                     FIRMASLWKRLFYAPGQRRRYFEQGERSFSIICGKLRGIVLTIKCSNGTIYLSIT"
BASE COUNT         1211 a          769 c          977 g         1327 t
ORIGIN      
        1 tgattttaat ggtttttctt tttttattga aataattgat agtaatgtgt ttattacgtt
       61 tgccttagag tataacgaaa atgcgttttt ttcttttttc aatgcccttg cccctgaacg
      121 gacgcaggga gtgattgaac atatttttgt ctacgataac aaattgtgct taagttgttt
      181 gttaaccaat attgatgtgt tttctctaat gaatactttt cagcagcatg tgcagattat
      241 tgaacgagtt cgcaggatga catcatgaat aaactcttaa atatatttaa aaaagcagag
      301 tcatatcacg atcttattct ggctctcttc ttctttatgg ctgtaatgat gatgattatt
      361 ccattgccaa cagttgtggt ggatatcatt atcgcgatta atatatcgac agcgttgctt
      421 ttattaatgc tctcaatcta tataaaaaat cctcttgagc taacttcttt tcctacaatc
      481 ctgttgatta cgacgttgat gcgcctgtcg ctcagtgtta gtacaaccag acttattctg
      541 ctgcatcacg atgcgggtga cattatttac tccttcggta actttgttgt tggcggtaat
      601 attgttgttg gcctggttat ttttaccatt atcaccattg tacaatttat ggttattaca
      661 aaaggggctg agcgtgttgc tgaagtgagt gctcgttttt cccttgatgg tatgccagga
      721 aaacaaatga gtattgatgg tgatatgcgc gcaggcgtta ttgatccgct tgaggcaaaa
      781 gttcttcgct cccgagtgca aaaggaaagc cagttttatg gttcaatgga cggggcgatg
      841 aagtttgtaa aaggggacgc tattgccggt atcatcattg ttttagttaa cctgtttggt
      901 ggcgtgctca ttggaatgtg gcaatttgac atgccattta gtgaagcact tagcctgttt
      961 tctgtattgt ctgtcggtga tgctttggtt gcccagatcc ctgcgcttat tatttccgtc
     1021 actgcggggg ttgttgttac tcgtgttccc ggtgaaagcg aaaaagaaga aaaccttgca
     1081 ggtgatattg ttcaacaggt ttctgttaat agtcgccctt ttttaatcag tgccgcactc
     1141 atgatcgtaa tggcgattat tcctggcttt cctacattgg tcttcttgtt tctggcggtt
     1201 tgcctgttgg gtatcgcctg gaaattacaa aagaaaagaa cgtttggaac cggaaataat
     1261 aaggacgcta tgggagctga tttgtctaat agtcaaaata tctcaccggg tgccgagccg
     1321 ttgattttaa acttaagtag taatatttat agctcagata ttacacagca gattgaggtt
     1381 atgcgttgga atttctttga ggaaagtgga attccattgc ctaagattat tgttaatcca
     1441 gttaaaaata atgatagcgc tatagaattt ttgctctatc aagagtcaat atacaaagat
     1501 actcttacag atgatactgt ttattttgag gctgggcatg cagagatatc atttgaattt
     1561 gtccaggaaa agctttcggc taactctatc gtgtataaaa cgaatgaggc taaccaacaa
     1621 ctagctcacg tctcaggtat ggatgtttat gcaaaaacaa atgacaagat aacgtttttg
     1681 ctaaagaaac ttgtattgtc taatgccaaa gagttcatcg gcgtacaaga aacgcgttat
     1741 ctgatggaca tcatggagag gaaatataac gaacttgtga aagagcttca gcgtcagctt
     1801 ggtctgagca agattgtgga tattcttcag cgtctcgtag aggaaaatat ctcaattaga
     1861 gatctacgaa ctatttttga aacgcttatt ttttggtcca caaaagaaaa agatgtggtt
     1921 atcttgtgcg aatacgttcg tatcgccctc cgtcggcata ttttaggtcg ctatagcgtt
     1981 cgcggtacac ttctgaatgt ctggcttatt ggctctgata ttgaaaatga gttacgagag
     2041 tcgatcagac aaacgtcatc aggttcgtat ttgaatatct caccggagcg aagtgagcag
     2101 attattggct tcttaaaaaa tattgtgaat ccaacgggga atggcgtcat tctgaccgct
     2161 ttagatatca ggcgctatgt gaagaaaatg attgaaggtt cgttcccgtc agtccccgtg
     2221 ctctcttttc aggaagttgg gaataatatc gaacttaaag tattaggaac ggtaaatgat
     2281 tttagagcat gattctgtat tggaaagata cccacgtatc cagaaagtgc tcaatagcac
     2341 tgtgccgaca ttatcattaa attcgtctac cagatatgaa ggcaagatta ccaatatcgg
     2401 cgggacgatt atcaaggcgc gtttaccaaa agcgcgtatt ggtgcctttt acaagataga
     2461 acccagtcaa cgtttagctg aggtgatcgc tattgatgaa gatgaggttt tcctgctgcc
     2521 ttttgagcat atttccggca tgtattgtgg ccagtggcta agttatcagg gggaagagtt
     2581 caagattcgt gttggggacg aactccttgg gcgactggtt gatggtatag gcagaccaat
     2641 ggggagcaat attactgcac cttatcttcc gtttgaacgc agtttgtatg ctgaaccccc
     2701 cgatccttta ttaaggcagg ttattgatca gccatttacg cttggcgtca gggcaataga
     2761 tggtttactt acttgcggta ttgggcaacg catcggtatt ttcgcggggt ccggcgttgg
     2821 taaaagtacg cttttgggaa tgatttgtaa tggcgcatcc gcggatatta ttgtccttgc
     2881 ccttatcggc gagcgtggtc gcgaagtaaa tgaattcctc gcgcttttac ctcaatccac
     2941 gctttctaaa tgtgtactgg tcgtcacaac gtcagaccgt cccgcgctgg aaagaatgaa
     3001 ggccgcattt acggcgacaa ccattgcaga gttcttccgg gatcagggca aaaatgtatt
     3061 attaatgatg gattccgtaa ctcgttatgc ccgagccgcg cgtgatgttg ggcttgcatc
     3121 gggagagcct gatgtaagag ggggattccc tccgagtgtt ttttcctcgt tacccaaatt
     3181 attagagcgt gcgggacctg cgccaaaagg ttcaataact gcgatttata cggtgctgtt
     3241 agaaagcgat aatgtgaatg atcccatcgg tgatgaagtt cgctctattc ttgatggaca
     3301 tattgtgctt acccgagaac ttgcagagga aaaccatttc cctgcaattg atattggttt
     3361 aagcgccagt cgtgttatgc ataacgttgt taaatcggaa catttacgtg cagcggctga
     3421 atgcaaaaag cttattgcaa cctataagaa tattgaactg cttattcgta ttggtgagta
     3481 cacgatgggg caagatcctg aagcggataa ggcaataaaa aacaggaaag caattcagag
     3541 ctttatacaa caaagcacca aagatatcag tagttacgaa aaaacgattg aaagcctatt
     3601 caaagtggtt gcctgaatat gttggacaga attttatcta ttcgtaaaag cagagcgaat
     3661 cgattgagag aatcaatggc taagattaac tctcagataa aagaggtgga tggtaaactc
     3721 gatgactgcg agcaagccat taaagagtcg attgcctcta agcaggcgta ttgtgcatcg
     3781 ctggtgaatc tggataaagt gtcgttgtat aaataccaga ttaaaaacaa tgcatttgat
     3841 gagcaaaagc agcgattata tgagaaaaaa tcctcacttt ctaaagaaaa gcgttcattg
     3901 ctcgattcac aaaaacgaac aaaagaggat ctacaacatg ttaataaatc aatagagaaa
     3961 ttatcttttg ccataaagga acattacttt gactagcatt tctctaaaaa gaaatttgat
     4021 agagcctgtt tcttttcagc cgtcagatga ttggccgcag ggagaagaaa gttttaatga
     4081 tatttttaat cgcataaaaa aaagaacacc tgacacttcg ttcatacgaa tggcttcttt
     4141 atggaaacga ttgttctacg ctcctggaca gaggcgacgc tactttgaac aaggagagcg
     4201 ttcatttagt attatttgtg gaaagttacg cggaattgtt ttaacaataa aatgtagtaa
     4261 tggaaccatt tatctgtcta ttac
//
LOCUS       DAZFKM010000242         4267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFKM010000242 DAZFKM010000000
VERSION     DAZFKM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..67)
                     /locus_tag="ACHKAY_005041"
     CDS             complement(<1..67)
                     /locus_tag="ACHKAY_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703537.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
     gene            complement(64..639)
                     /locus_tag="ACHKAY_005042"
     CDS             complement(64..639)
                     /locus_tag="ACHKAY_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703538.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(654..1007)
                     /locus_tag="ACHKAY_005043"
     CDS             complement(654..1007)
                     /locus_tag="ACHKAY_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5703539.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKAY_005044"
     CDS             complement(1000..1374)
                     /gene="gpFI"
                     /locus_tag="ACHKAY_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5703540.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1426..2310)
                     /locus_tag="ACHKAY_005045"
     CDS             complement(1426..2310)
                     /locus_tag="ACHKAY_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5703541.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVASSRYPKHWLTVGDPAREFTMTQS
                     APLMVLPDPDEFVVVQVK"
     gene            complement(2368..2715)
                     /locus_tag="ACHKAY_005046"
     CDS             complement(2368..2715)
                     /locus_tag="ACHKAY_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5703542.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(2752..4257)
                     /locus_tag="ACHKAY_005047"
     CDS             complement(2752..4257)
                     /locus_tag="ACHKAY_005047"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5703543.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          907 a         1340 c         1033 g          987 t
ORIGIN      
        1 gcgtcgcccc gtgttcatgc tgctcgagtg catccagcac tgcagcacgg atatcagtat
       61 gtttcatcgc tttatcacaa tccttagttg ttgtttcaac gcatagccca gttcttttgg
      121 cagacgttct cgccggatac ggttaacgtt ctcatcaaaa gcctgcttca gtggggccgc
      181 catcggaatt ttaaccacct gaatgggaag acgattacgc tttttccttc ctttatcgtc
      241 attgcccttc gcataccggg cttctggcaa acgttgcata acatgccagc gcccattatt
      301 taatcgctgg atgaatgccc gctgataacg atgctgaccg gctttaagta tgctgttcgg
      361 acgatgacca agcatcctga tccccagctt aatagcaggg agatcaccgc ggttaacgat
      421 aattctggca ttcggatttc tgaccgtcgc ccgtttcagt ctggaccgtt cctttaccag
      481 tttccggctc accctggtct cccgggcaac ctgtgatgaa gactgattaa tcgccgttgt
      541 agccacgcgg ttaatagcca ttgctgaagc cgacggaatg gcgtttttac gaacccggct
      601 cagattttca atcgcctgat caagcccttt tatcgccata attcaccctg cgtttatcgt
      661 cgccggttaa ctgcgggtgg ttgcccacgg ttcagccaga gataacaact gcccccgtca
      721 tccggagaaa cacgatccac ccagaacgtc tcaccattaa tggtcaacgt gtcaccacgc
      781 cgcacggcac gaaccgtatc cgtccgcaca aataatgacg ggctgcttcc ttcaatacgg
      841 atcccactac tggcaaaccc cagcgactcc ggatcgtcaa aaaccccctg aacttcgccg
      901 ccacgctgtg caccggaggt gaactgcgca ctgatgccca tcacttcaac aatcgtactg
      961 tccaccccgg caagggcggc atcaaaggca ttctgaaaat cacgcataaa cagccattcc
     1021 accatcaacg tgtgtttttg catctgagga cataatcaga atcacccgac caacatccgc
     1081 aagctcaacg gattcccccg tttcaccatc aacgccacag agatggaggc aggtcagaac
     1141 tctgatgcgc gttaacgcgc cggatgtttc ctcacgaaca tcatgagccg cgttttccct
     1201 ctcccggata tccatattca taacctgtac atcatcgccg gatgactgca tttcctcttc
     1261 ccattctgcc acccgctgcg ctatctctgc ggcactcccg gatatatccg gctcacgccc
     1321 cagaatcagg gccagttcat caagccgttt cagattttgc tctttcgttg ccatatcatc
     1381 cccctgtgaa aaaagacacg ggggcatttc gcccccgctc acggattatt tcacctgtac
     1441 caccacaaac tcatccgggt ccggcaacac catcagcggc gcggactgcg tcatggtaaa
     1501 ttcacgggcg ggatccccta ccgtcagcca gtgtttcgga taacgggaag aggccaccac
     1561 aatggccaga tcgccgtaat accctttgaa ggacaccacc gcgcccaggt ctttcaccgc
     1621 tgtttcgagt tgcgaattag agccgcgacg ggtatccagt ttttcgcgga acaatttaaa
     1681 gccattcagc agacgccaga cggtaccgtc cataatggca atattcacaa gaccgctggc
     1741 ctggtcgcag tagaggtcaa gatcatgtgt aggatcgaac gtgtcacgat cctgttttga
     1801 ccactcctta ccactaccct gagtgatgtt attcttcgtc gacctgccaa aatcgacctc
     1861 aattttctcg aactggtctc cttccatcgt gtatttgcca tacagcacgg cattcaccgc
     1921 ctgcatttct tccacccgga caatggcgtg ctcttcctgt ttgaggttat cagtgatgat
     1981 acgcagacga cggtaggccg ggtcgttcag ctgagccgga tcttcaccgg gaagacgctc
     2041 aaccgcctgc tggtaattaa attcgtgttt cggcttgacg tagcccggac gtaacacgcg
     2101 ggtttcacca ccacgatgac gcagcacttt tccttcaaca accggggaaa cataggccgc
     2161 caccggcgtt tttccggtaa ttttgtccag catcacctct tcggtatgga aattcaccgt
     2221 acggcggaaa aacagctcca gaaacagcgc acgaaatttc actttttgtt cggtataacc
     2281 gagtaactgg cgggtcgtaa acaatcccat aaatcagttc ctttcattca gaaatcagtc
     2341 aggccaccgc ggtggcctga taacgtgtta cggcagcgcc gcgtgactca gggcactgcc
     2401 ggcaaaggcg ttggcctttt tgtgttcatc cacactttca ggccagcgga ttgcctccgt
     2461 cgcaaaggtc cccgacttgt aataggtcag cgccgtctct gtgccttcaa gcggcagtac
     2521 cagtatgcca accgcactac cggctttctg tccgtcccag accaccagtt tcccggtggc
     2581 ctcatccagc atcaggggcg tcagtgccgg tgttgccgag gaaatcccgc tgctgcctgt
     2641 ggcggtatga gccggatcat taccggcaaa aatacgtact tccacacgct gttcagtgat
     2701 agttttcgtc accatattgt aaaaacctcc tgttgatggt cagcactggc ttcatggcat
     2761 ggccatgagc attttcacgt ccgcatcacc gtctgctgac gtctgtggca cgccaccctg
     2821 taccgctgcc ggtgaatggt tcgccatgat gcgttcaaac atggcggttg tggatgcaga
     2881 gaccggttct gccttacctg atcccgcagc cagcacagcc cgggcgctct ccacagtcat
     2941 tcccgggcag gcagccagct gttcagcctg cgcctcagcc ccttttgcct catccagtgc
     3001 catgatctga tcacggagtg agggtccggc atccgcctgc ggtgaagcag ccaggatcgg
     3061 gcgggctttt tccaccgtca tctccggcat cgccgccagc gttgccgcca gttgttcacg
     3121 acctttcgct tcttcacacg ccataatgcg atcggcttca ctctgcgcgg atgccaccgg
     3181 ctgctgtggt gccgccgcgg ccagaatcgc ccgggcctgt tcaacgctca tgccctgttg
     3241 tcctgccagc atcgtggcaa gctgttcacg tcctttcgct tcctggcatg tcaggatccc
     3301 catcactcgc tggttctcct gcgcggcggc ttccgttgca gttaattgcg gcatagtgcc
     3361 tcctctgaca ttactgttca gcgccgtggc catcacactg atggcatccg acgcattgat
     3421 taattcatcc gccagcccgg cctcaatgcc ggactgacct tcaaaaacgg cggcctctgt
     3481 tcccgtgaca gcatcaacag acagaccggt atacatcgct actttttcag caaacatccg
     3541 gcgcgccgca tcaatgcgct gttgcatgtc ctggcgaacc tctgccggca acgcttcaaa
     3601 ctgattgcca tccaccttgt gcgcccctga gtaaatcagc gtgatatcaa caccggcctg
     3661 cgccagatga ccggcatagc tgacatggct catcatcacg ccaatggagc cgatacggga
     3721 tgtctgggta accagccgtc gggagcaggc cgacgccagc agcatggctg cagagcaggc
     3781 cgtgtcattg cacagtgccc agaccggctt ctgctgacgg aggcggtaaa tcatgtcagc
     3841 gcagtcaaac gcgccggcgg cctgcccgcc cggactgtca atgtccagca gtacgccccg
     3901 cacctggcta tccgccattg cctgctgaag acaggcgaca atgccgtcat agcctgtcat
     3961 tccggaaaat ggccgcatac cccccagccg gtgcaccagc gtgccggtca ccggcagtac
     4021 agcaataccg ttcaccaccc ggtaaacacg ggccggtcgt ttacctccgg ccatgtactc
     4081 gtccgtttca gccagcattc cgggagcatc aaactgtacc tgctgttgtg gtaccgaaag
     4141 acttgatgcc cccatctcgc gcccgagcgc gcaaaagaaa acccgcgcat aggcgggctc
     4201 cagaagcagc ggttcattga atgctgcggc aataatgtgt gaaagattac gtctcacgtg
     4261 gtgttgt
//
LOCUS       DAZFKM010000243         4097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFKM010000243 DAZFKM010000000
VERSION     DAZFKM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..1710
                     /gene="dpiB"
                     /locus_tag="ACHKAY_005048"
     CDS             52..1710
                     /gene="dpiB"
                     /locus_tag="ACHKAY_005048"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO5703544.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1679..2359
                     /gene="dpiA"
                     /locus_tag="ACHKAY_005049"
                     /gene_synonym="citB"
     CDS             1679..2359
                     /gene="dpiA"
                     /locus_tag="ACHKAY_005049"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO5703545.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2400..3785)
                     /gene="dcuC"
                     /locus_tag="ACHKAY_005050"
     CDS             complement(2400..3785)
                     /gene="dcuC"
                     /locus_tag="ACHKAY_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO5703546.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1127 a          957 c         1052 g          961 t
ORIGIN      
        1 cattttaatt acatccgtca aagaggctcg ggacaacccg caaggaaaac aatgttgcag
       61 cttaacgaga ataaacagtt tgcatttttc caaagactgg catttccgct gcgtatcttt
      121 ttgctgattc tggtgttctc aatatttgtc attgcagccc tggcgcaata ttttacggcc
      181 agttttgagg actatttaac gcttcatgta cgcgacatgg caatgaatca ggcgaaaatt
      241 attgcctcca atgacagtgt catctctgcg gtgaaaacgc gtgactacaa acggctggcg
      301 accatcgcta acaaattaca aagagatacc gattttgatt atgtggtgat tggggaccgg
      361 cactcgatcc gcctttacca tcctaatccg gagaaaattg gttatcctat gcagttcacc
      421 aaacagggcg cgctggagaa aggggagagc tacttcatta ccgggaaagg gtcaatgggg
      481 atggcgatgc gcgccaaaac gccaatcttt gatgacgatg gaaaagtcat cggcgtggtg
      541 tcgattggct acctggtgag taaaatcgat agctggcggg ctgagttttt attaccgatg
      601 gcaggcgtgt ttgtcgtgct gttagggatt ctgatgttgc tgtcgtggtt cctggcagcg
      661 catatccgtc ggcaaatgat gggcatggag ccaaagcaaa tcgcacgcgt ggtccgtcag
      721 caagaggcgc tgtttagttc ggtttatgaa gggctgattg cggtggatcc gcatggttac
      781 attaccgcca tcaatcgtaa cgcaagaaag atgctggggc tgagctctcc cggacggcaa
      841 tggttgggta aacccattgc tgaagtggtc aggcccgccg atttctttac cgaacagatt
      901 gatgaaaaac gtcaggatgt ggtggctaac tttaacggtc tgagcgttat tgccaatcgg
      961 gaagctattc gttcaggtga tgatttgctg ggggccatta tcagctttcg tagtaaagac
     1021 gaaatttcca ccctcaatgc gcaactgacg caaataaaac aatacgtcga gagccttcgt
     1081 acattgcgac acgagcatct caattggatg tcgacgctca atggtctgtt gcagatgaaa
     1141 gagtatgatc gcgtgctggc gatggtgcag ggggagtctc aggcccagca acagcttatt
     1201 gacagcctgc gtgaggcgtt tgccgatcgc caggtggcgg ggctgctttt tggtaaagtg
     1261 cagcgcgccc gggaactggg gctaaaaatg atcattgtcc ccggtagcca gctttcgcaa
     1321 ctgccgccag gactggatag caccgagttt gcagccattg tgggcaattt acttgataac
     1381 gccttcgaag ccagcctgcg tagcgatgaa ggaaacaaga tcgttgaatt attcctcagc
     1441 gatgaaggcg atgatgtggt gattgaagtc gccgatcagg gctgcggcgt tccagagtct
     1501 ctacgagaca aaatatttga gcagggggtc agtacgcgtg ctgacgagcc cggtgaacat
     1561 ggcattgggt tgtacttgat tgccagctac gtaacgcgct gcggtggtgt tatcactctc
     1621 gaagataatg atccctgcgg taccttgttt tcaatctata ttccgaaagt gaaacctaat
     1681 gacagctcca ttaaccctat tgatcgttga ggacgaaacg ccgctggcag agatgcatgc
     1741 ggaatatatt cgtcacattc ccggattcag tcagatatta ctggcgggaa acctggcgca
     1801 ggcccgaatg atgatcgagc gttttaagcc ggggctaatc ttgctcgata actatcttcc
     1861 tgacggcaga gggattaatt tattgcatga actggtgcag gcgcattatc ccggcgacgt
     1921 ggtgtttacc actgcagcca gcgatatgga aacggtgtct gaagcagtac gttgtggtgt
     1981 atttgattat ctcattaagc ccattgccta tgaacggctg gggcaaacgc taacccgttt
     2041 ccgccagcgt aaacatatgc tggaaagtat tgatagcgcc agccagaagc aaattgatga
     2101 gatgtttaat gcctatgctc gcggtgaacc taaagacgaa ctgccgaccg gcattgatcc
     2161 cttgacgcta aacgcggtgc gaaaactgtt taaagagcct ggtgtgcaac atacggcaga
     2221 aacggtggcg caggcactga ccatcagtcg caccactgcc aggcgttatc ttgaatattg
     2281 cgccagccgc catctgatta ttgctgaaat tgttcacggc aaagttggca gaccgcaacg
     2341 catataccac agtgggtgac atgataaagc cggaggaaac ttgcctccgg cattactatt
     2401 tacttgcctg tgaccgctgc tgccgttcct ggcaccatca gctctgtagc aacgataaca
     2461 atcaccaaac caacaagcac cggtaccgag gtgcgtttta cgacttcaaa cggcgagatc
     2521 ttcgccatcc cggcaaccgc aacgactacg ccagaaacgg gcgaaagggt acggcccagg
     2581 tttgacgcct gcagcatcgg gatagtcaaa tacgccgggt taatgccgga agagtgcgcc
     2641 agtttcggga tcatctcaac aaacgcataa aacggcgcat tgcctgaacc ggtcgtgact
     2701 gccgccagca tggtcagaat caccaatacc agcatcagga tgatactcgc cgaaccaaac
     2761 gaggtagcga tagaaatcag actttgaata aagccgatgg tgctaagccc ctgagcgaat
     2821 accccagcgg caaccagcag catcaccacg ttagcaaacg catctgccat tccgcgataa
     2881 gccacttcca gaccagagaa aactttctgg gtattaaagc tgcggaggaa ctccagaatg
     2941 gaggcaatca gcatacaaat caccagaata gtgatgatgt gtaattgcgg accccattta
     3001 ccgtcaaaaa tcagtacacc gatgatcggc gtgaacggca aaatggcata aaacgcagga
     3061 gcagtggtgg tgatttcact gacatctaac atttcatgag agatgtgctc ttttttatcc
     3121 agataacgtt gccagaagaa gtgggcgatc gccatgccga taattgcagc aattgagata
     3181 ggcagcgtcg ttttgaaggc gaagtcaatc agcgacattt cggaagcttg cgccgccagc
     3241 accacatccc ctgaagtcgg tgcgagaata atcgccgccg gggaggcaca aatggcagca
     3301 gctgcgccac gactgatacc aacgtttacc atcaccggaa atagggttgc catcagcaaa
     3361 acacccagac cggttgcgga agagacggcc agagacatca gacaggcgac aaaataggcg
     3421 gcaatcatca gcaggtaagg ggagttaata tactgcaatg gttttgacgc cagcttgacc
     3481 accatatcat tcgcgccgat atgggtcatg taagcggcaa atccacacag catcataatc
     3541 atcatgccga ggtcgccgcc gcggctcatt agtaatattt taacgtattc aacgatatcc
     3601 gtggcgctgt agcctgttga agcctggctg gacggtaaca ctttgtgccc cataatggca
     3661 ctgataatca gcaataacag gccaccgaca aataacacac cagtggcgga atacccttta
     3721 atgatgtagc gagctacacc cacaataacc acaaccccaa taaggagttc aatgaatgtc
     3781 agcataattt ttcctgtctc caggccccaa agtaaataat aaaaaattcc taaagcttaa
     3841 ggaaaaaata tgcccaataa attggcgatg aatgctgatt aaaatcaaga aaaactgcca
     3901 ttaagacatt gaagttgctg tttttataca tagataacaa aacgcatatt tagcggttgg
     3961 caattatcac ataataatgt gtgtaaattg atggtggatt gatgcaaatt tgttaattgc
     4021 aatggtgtta gctgcggctg tgcgctcaaa aaataatcta acatgagcat aggttgacga
     4081 tttatattat atacttc
//
LOCUS       DAZFKM010000244         4092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFKM010000244 DAZFKM010000000
VERSION     DAZFKM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1233
                     /locus_tag="ACHKAY_005051"
     CDS             <1..1233
                     /locus_tag="ACHKAY_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5703547.1"
                     /translation="PKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAG
                     QCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNV
                     PTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAWARRGIISDRLATAILRHA
                     GRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGGRAEV
                     VSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYV
                     MAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGA
                     FDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRNATY
                     PVLIDSIAVEVNSTDTAA"
     gene            1369..1548
                     /locus_tag="ACHKAY_005052"
     CDS             1369..1548
                     /locus_tag="ACHKAY_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5703548.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            1589..1834
                     /locus_tag="ACHKAY_005053"
     CDS             1589..1834
                     /locus_tag="ACHKAY_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5703549.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            1912..2127
                     /locus_tag="ACHKAY_005054"
     CDS             1912..2127
                     /locus_tag="ACHKAY_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5703550.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2132..2665
                     /locus_tag="ACHKAY_005055"
     CDS             2132..2665
                     /locus_tag="ACHKAY_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5703551.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2940..3509
                     /locus_tag="ACHKAY_005056"
     CDS             2940..3509
                     /locus_tag="ACHKAY_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5703552.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            3509..3658
                     /locus_tag="ACHKAY_005057"
     CDS             3509..3658
                     /locus_tag="ACHKAY_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703553.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            3666..4031
                     /locus_tag="ACHKAY_005058"
                     /pseudo
     CDS             3666..4031
                     /locus_tag="ACHKAY_005058"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            4000..4071
                     /locus_tag="ACHKAY_005059"
                     /pseudo
     CDS             4000..4071
                     /locus_tag="ACHKAY_005059"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
BASE COUNT         1046 a          927 c         1208 g          911 t
ORIGIN      
        1 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
       61 ccggcaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      121 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      181 acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctat
      241 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggggtg
      301 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
      361 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagc
      421 gcctgggcac gccgtgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg
      481 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccttcatcg
      541 ccttccggtg cagaagccac aggcgtcaca acactgctct cttaccttgc cagcgagtca
      601 gagggaagcc tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc
      661 gatgcggagg gaaccggagg taaggcagtg aagctgacca aggaggccgg taaaagcagc
      721 tgggtgctgg agtacgccgc gggcaacggt gcggctctgt tacagaaagg ggggcagatt
      781 cgctgccgct ttaaggtttc gggagcgctg gctgcgaacc agtatgttat ggcgttttac
      841 tggccggtct cttcactgcc acagggcgtt gccctgaccg gagacggggg gaataacctg
      901 ctggcagcgt tctacatcca gacagatgca aaagacctga atgtgatgta ccacaatgcg
      961 aaagtggcga caaacaacct gaaactggga acctttggcg catttgataa cgaatggcat
     1021 acgctggctt tccgctttgc cgggaataac agccttcagg tgacgccggt tattgatggt
     1081 caggatggca caccgttcac gctgacgcag tcaccggtca gtgcatttgc ggcggataaa
     1141 ctgcatgtga cagacattac caggaatgcg acttacccgg tactgataga cagcattgcg
     1201 gtggaagtga acagcacaga cactgcggca tgataaaaaa aaccgccagc gacaggaatg
     1261 gacgctggcg gtggtgatac ctatggagaa aaaataaagg aacgatactt tcgtactctg
     1321 gtttttaatg aaaacagttc ttattgtcaa caataacgga aagaaattat gacatttctg
     1381 aaccagttaa tgctgtactt ctgtacggtg gtctgtgtgc tgtatctcct ttcgggtggg
     1441 tacagggcag tgcgcgattg ctggcgcagg cagattgaca aaagggccgc tgagaaaatc
     1501 agcgccggtc agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct
     1561 taatcacacc tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga
     1621 agcagtgaag gaagtcctgc gctctgaaga agtccggagc gcactgaaac agaaacttcg
     1681 ccataacctg gaagcgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
     1741 cgcaccggca gctccggagc cggaaggcat cgcgggtgag gggagtgctt cagatagcgg
     1801 tgaccccaca ccggacagcg acatgatgat gtaagcatgc gtcagggacc atcggtgtgt
     1861 gccggtggtc ttttttattg ttgtgagctt ccggattgcg ggaggcgggg tatgaaccag
     1921 atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtgggaac gggctactgg
     1981 ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg
     2041 agtctgctgt ttggtctgct gacgtacctg acgaacctgt atttcaaaat cagagaggac
     2101 aggcgtaagg cggcacgggg agagtaagct gatgagcagg aaactccgct atggtttatc
     2161 ggctgccgtt ctggcgctga ttgccgcagg tgcttctgcg cctgaaatcc tcgaccagtt
     2221 tctggatgaa aaggaaggca accacaccac ggcataccgt gatggtgcgg gtatctggac
     2281 catctgccga ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc
     2341 gaaggaaaaa tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga
     2401 gcgtaatata aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc
     2461 ctataacatt ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg
     2521 cgatcgcagg ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg
     2581 ccgtatccgt tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct
     2641 ggcatgctgg ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac
     2701 gcgggtagac aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag
     2761 attgtttcat gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg
     2821 taaataaacc aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt
     2881 aaccctcttt gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga
     2941 tgaatatgat gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg
     3001 aaccatacgt tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt
     3061 tggctaagtt aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg
     3121 atgggaaacg acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa
     3181 ccatcaatcc caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag
     3241 agtgtgacga tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa
     3301 tgagtgtgat ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg
     3361 ccagtgtgtt tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa
     3421 aaatccgcac attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag
     3481 acaaagggaa aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact
     3541 cataacgctg gcgaagatcc tctgtgtaat cgtcggcatt tcatttttag tcattctggt
     3601 tgccattttc ttttccaccg cttggcgagt cctgacgtta tcgggactgg tggggtgaaa
     3661 gagagatgaa ccgtgttctg tgtgtggtga ttattgtcct gctggtagcc tgtggtgcac
     3721 ttagtctggg gctgaatcat taccgcgata acgccataac ctacaaagag cagcgcgata
     3781 aaaaagtcag tgagctggag ctggcaaatg caaccattac tgatatgcag cagcgccagc
     3841 gtgatgttgc tgcacttgat gccagatact cgagggaatt agccgatgcg agagctgaaa
     3901 atgaaactct gcgtgctgat gttgccgctg gtcgtaagcg cctgcgcatc aacgccaact
     3961 gtccaggctc cttgcgtaaa gcccccatca cctccggcgt ggataatgca accggtcccc
     4021 gactggcaga agccgctgaa cgggattatt tcatcctcag agaacggctg atggcaatgc
     4081 agaagcaact gg
//
LOCUS       DAZFKM010000245         4063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFKM010000245 DAZFKM010000000
VERSION     DAZFKM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..548
                     /locus_tag="ACHKAY_005060"
                     /pseudo
     CDS             48..548
                     /locus_tag="ACHKAY_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            550..843
                     /locus_tag="ACHKAY_005061"
     CDS             550..843
                     /locus_tag="ACHKAY_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO5703554.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(904..2024)
                     /locus_tag="ACHKAY_005062"
                     /pseudo
     CDS             complement(904..2024)
                     /locus_tag="ACHKAY_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(2033..2800)
                     /gene="sctT"
                     /locus_tag="ACHKAY_005063"
     CDS             complement(2033..2800)
                     /gene="sctT"
                     /locus_tag="ACHKAY_005063"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO5703555.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(2802..3062)
                     /locus_tag="ACHKAY_005064"
     CDS             complement(2802..3062)
                     /locus_tag="ACHKAY_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5703556.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(3072..3737)
                     /locus_tag="ACHKAY_005065"
     CDS             complement(3072..3737)
                     /locus_tag="ACHKAY_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO5703557.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(3727..4063)
                     /locus_tag="ACHKAY_005066"
                     /pseudo
     CDS             complement(3727..4063)
                     /locus_tag="ACHKAY_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
BASE COUNT         1380 a          803 c          635 g         1245 t
ORIGIN      
        1 ttacacgtaa tatttaccct aaatgatttt agagtacagg tgggcatatg caagtctttt
       61 ccagtgatgt ctacttcact gtaggcacta atgcattatt agcatcccaa aaagaatatt
      121 acagcgattt agttgcgttg gttgatcttg gacattcgtt cgttgttatt gatgagcatc
      181 aacatcgaaa tctcaaacca aatacagaac ctgtaaacat ccttttgagc aacaatttca
      241 tcaggatcaa taagaatatt acattatctg atttaacgca tttcctggtt tcaaatcttc
      301 atacacaaaa tgtgtattcc acacaagaac cacttaccca tgatgagata gatatcctgc
      361 ggttatgtgt ttcttactcg ttaaaacaaa tagccattat taaaggaatt gattataaaa
      421 caatatcata tcacaaaata agagccctaa ataaacttaa cataaaaggt actgtttagt
      481 tgtttatagc actctgtgaa tgggataaac attattttaa actccaatct tgcgtaagag
      541 aaagttgata tgtcattagt aggatataat agtaacatca taataaccat cgcgctgata
      601 tcgttgctct ctgcatgcac cagagaaaat tcattaaagg agaatcaaaa taaaagaagt
      661 ggcgaatccc aagcgatagt agatgcaaga cagttcttta gcgaacaccc ggaatatata
      721 tcatcagcag aactggagca aaagctatat cgcgaatttg cacaggttac tacagttcca
      781 gcttataaag agatgagtat gtatcaattg cttatcattt cacaagatcg tttaactcat
      841 taaataagga tctacacgct ttttgcgtgt agatccttat aaatttctga ctacgacaga
      901 tgtttaattt tttaaattat cgtctttatt ctcgattttt gtgtcttcac cctcaatgaa
      961 tttatcttct gaagagagct gttcatcttg aacaggttgc ccagcattct ccacatcttc
     1021 aagccacagc agaagacgta atatttcatc tatattttcg aagctaacat aatcgtagcg
     1081 acgatgggta gcataatttt ttcgtgctaa ttttttatct gtgataattg gtattccgat
     1141 ttcctttgca tatttcctta cgcctaaggc cacctcatta gttactctta cagaaatcaa
     1201 tggaataggt gaaagatgtg gcttaaagta tatccctatt gcaatgtgag tagggttgga
     1261 aatcatcaaa cgactattac tgacatcaga cttcaattgc tcagaaagaa tttcctgatg
     1321 cgctctctgc gtttagactt aatttctgga tttccttctt gctccttgta ttctctttta
     1381 acttcttgtt tatccatttt catatctttc ataaataaaa aatattcagc gataaaatca
     1441 aaaattaaga caattatcat actgccgaga caatacagta tgagaaggaa tagcatctct
     1501 ccccaatctg ataataaaga tatgatatct ccatcaagag ttttaaaaag cagtgattta
     1561 tgattactcc aaaatacttt gatcgccaat gcaaaaaaaa caatataaag aattgcttta
     1621 acaaattctt ttacggtttt taagccaaat atacgcttta agccatttac tggattcaat
     1681 gaatcaaact taaatttaac agcttcagtt gctaattgca ttttactttg aaaccaggag
     1741 acaagtattg ttgtgaacat gcaaaccagc aaaaaaggaa tgttcgtttt caaccaacca
     1801 acccccatag ctttaaaata gtcccaaata tctggtttag cgttatgatc aaggatgtat
     1861 tcaagaatcg acataatgtg atgcacatca aaaacatatc cgagatataa agtacccaca
     1921 agcattatta cagagatggt taagtctcta ctttatagaa attgcccttt tttagaagca
     1981 tcctggagtt tcttttgtgt tggcttttca gttttattgg ccatgaaaca ctctatttct
     2041 caatgtatag ttgcaattgt tctgggaaaa aggataacgg cattactcgt tcagccagta
     2101 ttggggcaaa ataaataatt aatattaaaa atgccagcgc acttttgaca gtcagtgaaa
     2161 tggcaaaagc atttagttgt gaagcataac gtgccagcaa tcccaggaca gcctcaccgc
     2221 ccagcataac ggcaatcacg gggcttgcgt aaacgatagt atgagtcatt atgttattaa
     2281 tatatgagaa taaaggctct aatttaggaa aattaaaatt gcctgaaggc cataaattat
     2341 aactttgcca aagagtttcc agaataaagt tcattccacc tttagtaaga taaaccgcag
     2401 cagagaaaag gttaaataat cttgctaatt ccgaggtatc gacacctgta gccggatcca
     2461 aagtactact taatgttgca ccgcgttggt tatcgataaa gctacctata gcaaggaaaa
     2521 tccaaaacgg ggatgctaat aagcatgcaa taaacagccc tatgataatt tctctggcag
     2581 cgaacaaagc aatatcgatt tcatttaagt tcaataattc atagtggtaa tgctgaacta
     2641 atgcacatga taccactatt attattgggt gacgaactac tgttggtata ttcccactgg
     2701 cgaaaaatgg taacaaatag aaagttggtg ccaatcggca aaaaccgagc gctgtagcag
     2761 ccagcagact atgcaactga tagagtatcg cctcccccat gctatcctct cgcgaatgct
     2821 aaattcagca tttcgatacc aaatgaatat aatttttcac cataccaccc gctcattaaa
     2881 aaaaagcaaa tacttacgca caataatttc acgccaaatg gcaacgtctg ttcttgcaac
     2941 tgcgttaccg tttgaaaaag ccctaccaat aaaccaacaa atgtcgcaac tgcaataggt
     3001 cctgcagaca taactaaaat cagatacagc gccctattcc ccgcaaaaac aatatcgtcc
     3061 atattttttt ctcagggatt aatggatagg tcaaaatatt gtaaaatgag cccttttgat
     3121 aacattgtcc aaccgtccat cgcgacaaac aaaattaatt ttatcggtgt agatatggtt
     3181 accggactca tcatcatcat acctaacgtt aacagaacgc tggatataac cagatccact
     3241 acgacaaagg gtaaatagat ataaaaacct ataataaatg cactttttat ctcactcaat
     3301 gcatatgcgg gcagtaacga gaaaattgaa atattatcat catcgattat ctcttcattg
     3361 tcttcagaac tattcacctt ctgaatcttt tcaaaaaagc taaccaactc aggttcagaa
     3421 tattttatca aatatgattt ataaccactc atgcctgtct caacaaaatt aacaaccgat
     3481 gctacattat tgaatgaaag attctcattc tgactgttat tatatatttc cttcccaacc
     3541 ggcatcatta cgaacattga caatagcaaa gcaaccccat taagtgtcat atttgatggc
     3601 acttgctgaa gtcccagcgc gtttcggaca ataacaaaaa caatcgagaa tttaataaaa
     3661 catgtcccgg atgctattat aaacggcaat agtgtaaata atgataatat cgctatcagc
     3721 gaaatgctat ttgacattat tattccctga taaccaagaa tgaatttcta cgcccaattt
     3781 atcatccact tcaacaagtt cgccatagcc agttagcgca ccatttacac gtatctctat
     3841 attttctcag attcaggaag cagagatatt attctttttg cacagagatc gtctatttca
     3901 taaagattaa ttattttctt accaagaaca aactcaattt ttacaggcaa ctcttcaaaa
     3961 ctattaatca tctgttcgtc attttcatca taaatctcac tctcgttttt tttgatatca
     4021 aaatcatctg tttcaaaatc ttcctcgtac tcaaaatgat cag
//
LOCUS       DAZFKM010000246         4057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFKM010000246 DAZFKM010000000
VERSION     DAZFKM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1820
                     /locus_tag="ACHKAY_005067"
     CDS             <1..1820
                     /locus_tag="ACHKAY_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5703558.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDIGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHKAY_005068"
     CDS             1817..2023
                     /locus_tag="ACHKAY_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO5703559.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..3621
                     /locus_tag="ACHKAY_005069"
     CDS             2020..3621
                     /locus_tag="ACHKAY_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5703560.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSNEQRDKLAGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3602..>4057
                     /locus_tag="ACHKAY_005070"
     CDS             3602..>4057
                     /locus_tag="ACHKAY_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5703561.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRD"
BASE COUNT          924 a         1053 c         1253 g          827 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgacgagc
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ttggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaagcatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 ttggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatctgaccg
     1561 aagcgcagca gctgacagct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaaa cgacgcaatg aggctctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcgctg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagattac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggtaaac gggtggcaac agtacagaaa gacggacgaa gggtggagtt
     1921 tacggccact tccgtgtctg acctgaaaaa atatattgca gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gacctgcagg attttatgta tgaaaacgcc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgcgaat atgctggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgagtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagctgcat caggatcata tcgtcgggtc ttttttccgg
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagaa tttgccgagg atgactgctg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggagaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaatccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc
     2701 ggcgggcgcg cctcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgaaag tgagctggat
     2881 acgcagtcag cgatggattt tattcttggc gcgaacagta atgagcagcg ggacaagctg
     2941 gcgggctgga ttggtgaaat tgccgcgtat tacgccgccg cgccggtccg gctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggtgac tctctgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cggactgggt
     3121 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     3181 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     3241 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta
     3301 ccttcaaaag cgcgtttcag ctttcaggaa gcccgcagcg cctgggggaa ctgtgactgg
     3361 ataggctccg gtcgtatggc catcgatggt ctgaaagaag tacaggaagc ggtgatgctg
     3421 atagaagccg gactgagtac ctacgagaaa gagtgcgcaa aacgcggtga cgactatcag
     3481 gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagccgg tcttaaaccg
     3541 cccgcctggg cggctgcggc atttgaatcc ggactgcgac aatcaacaga ggaggagaag
     3601 agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct
     3661 gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat
     3721 cagccgcctg acggatgcgg tgtccggcga cagcctgact gcccaggagg cactcgcgac
     3781 gctggcatta tccggtgatg atgacggacc acgacaggcc cgcagttatc aggtcatgaa
     3841 cggcatcgcc gtgctgccgg tgtccggcac gctggtcagc cggacgcggg cgctgcagcc
     3901 gtactcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga
     3961 tccgatggtg gacggcattc tgctcgatat ggacacgccc ggcgggatgg tggcgggggc
     4021 atttgactgc gctgacatca tcgcccgtgt gcgtgac
//
LOCUS       DAZFKM010000247         4046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFKM010000247 DAZFKM010000000
VERSION     DAZFKM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..210
                     /locus_tag="ACHKAY_005071"
     CDS             <1..210
                     /locus_tag="ACHKAY_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO5703562.1"
                     /translation="EVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALAL
                     VDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            279..500
                     /locus_tag="ACHKAY_005072"
     CDS             279..500
                     /locus_tag="ACHKAY_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5703563.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            580..948
                     /locus_tag="ACHKAY_005073"
     CDS             580..948
                     /locus_tag="ACHKAY_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO5703564.1"
                     /translation="MSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQESNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            1038..1415
                     /locus_tag="ACHKAY_005074"
     CDS             1038..1415
                     /locus_tag="ACHKAY_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO5703565.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            1412..1900
                     /locus_tag="ACHKAY_005075"
     CDS             1412..1900
                     /locus_tag="ACHKAY_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO5703566.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            1912..2109
                     /locus_tag="ACHKAY_005076"
     CDS             1912..2109
                     /locus_tag="ACHKAY_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5703567.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSTALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            2194..3060
                     /locus_tag="ACHKAY_005077"
     CDS             2194..3060
                     /locus_tag="ACHKAY_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO5703568.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEICEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            3132..3395
                     /locus_tag="ACHKAY_005078"
     CDS             3132..3395
                     /locus_tag="ACHKAY_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO5703569.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            3392..3718
                     /locus_tag="ACHKAY_005079"
     CDS             3392..3718
                     /locus_tag="ACHKAY_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5703570.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQL"
BASE COUNT         1082 a         1044 c          988 g          932 t
ORIGIN      
        1 gaagtgatta aacgcgccct gtatcacaat gccgctgccg tggtgctggc acacaatcac
       61 ccgtctggtg aagtcacacc cagcaaggca gaccgcctta tcacggaacg tctggtacag
      121 gcactggctc tggtggatat ccgggtgccg gaccatctga tagtcggtgg cagccaggtt
      181 ttctcctttg cggaacacgg cctgctttaa cccgtcaccg tcacaaccac atcacacctg
      241 ttttcacttt tatcttctgt cttcagaggt atcccattat gaaaatcatc acccgtggtg
      301 aagccatgcg tattcaccaa caacatcctg catcccgtct ttttccgttc tgtaccggca
      361 aataccgctg gcacggcagc actgaagcgt ataccggtcg tgaagtgcag gatattcccg
      421 gtgtgctggc cgtgtttgct gaacgtcgta aggacagttt tggtccgtat gtccggctga
      481 tgagcgtcac cctgaactga gtcaggaagg gcattctgat gagccgaatt atcgccacca
      541 ccggagcatt cttaaccgat tttctgtgag gactttatcg tgtcagacac attctccggg
      601 acaacgcatc ccgatgacaa tcacgaccgc ccctggtggg ggctgccctg taccgtgaca
      661 ccttgttttg gcgcccgtct ggtgcaggag agtaaccggt tgcattacct tgccgaccgc
      721 gccggtatca gagggcggtt cagcgacgcg gatgcgtacc acctggacca ggcctttccg
      781 ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc ccgccatcag
      841 cataccgtga cactgtatgc aaaagggctg acctgcgaag ccgacaccct cggcagttgt
      901 ggctacgttt atatggctgt ttatccgacg cccgaaacga aaaagtaact ctccggaata
      961 accttctgct ccggcctggt gttttcacca cgccactttc ccatttctca tctctgcata
     1021 tcaggaaaat cttcagtatg aaaacattac ccgacacgca tgtacgggag gcatcgcgct
     1081 gcccgtctcc cgtcaccatc tggcagatac tgctgtcccg actgctggac cagcattacg
     1141 gcctcacact gaatgacaca ccgtttgccg atgaacgtgt gattgagcag catattgagg
     1201 caggcatttc actgtgcgat gcggtgaact ttctcgtgga aaaatacgca ctggtacgaa
     1261 ccgaccagcc ggggttcagc acctgtaccc gctctcagtt aataaacagt attgatatcc
     1321 tccgggcccg ccgggcaacc ggcctgatgg cccgcgacaa ttacagaacg gtaaataaca
     1381 ttaccctggg taaacatccg ggggcgaaac aatgaaactg gccctgacgc tggaagccga
     1441 cagcattaac gtgcaggcac tgaacatggg gcgcattgtc gttgacgtcg atggtgttac
     1501 tctcgctgaa ctgattaacg tggtctgcga taacggttac tcccttcgtg ttgttgatga
     1561 atctgaccgg gcatcagcag agcgcacgcc accatctgcg gcccttaccg gcatacgctg
     1621 cagtaccgca catatcacgg aaacggacaa tgcctggttg tactcgctgt cacatcagac
     1681 gaatgacacc ggcgaatcag aatggattca tttcacaggt agcggatatc tgttacgtac
     1741 cgatgcgtgg tcatacccgg ttttgcggct taagcgcctg ggcctgtcaa aaacgttccg
     1801 ccgtctggtt gtcacactta cccgacgtta tggcgtcagt ctcattcatc tggatgccag
     1861 cgctgaatgc ctgccgggtt tacccacttt caactggtaa ccaggaacaa catgaaatca
     1921 ttaaccacgg aaacagcgct ggatattctg attgcgtggc tgcaggacaa tattgactgc
     1981 gaatcggaaa ttatctttga caacgatgag gataaaacgg attcgacagc actgctgccc
     2041 tgcattgaac aggccaggga agatatccgt accctgcgcc agttgcagtt tctgcaacag
     2101 aaccggtgag tcttactcac cagacttcat tccactgacg tcagcctgaa tacggctggc
     2161 gttttcgttt ataaaccaaa aggaaaaacg atgatgtcag atatcacagc ctcccgtccg
     2221 gaagtggtca ccgggcatac ggacgttatt tgctcaacca gtatccgcca cattctggct
     2281 gtacgaaaaa gtacactgct gcaaatcgac acacttatcc ggcaactggc tgaaatatca
     2341 gcaatgacag aaagtattgg aggtaaaacc gcccgggact gggccatgaa acaggatttt
     2401 cgctgcggct gctggctgat ggagaaaccg gaaaccgcaa tgaaagccat cacccggaat
     2461 ctcgatcgcg aactctggcg tgacctgatg caacgttcag ggatgctgtc attaatggat
     2521 gcacaagccc gtgatacatg gtaccggtca ctggagtacg ataattttcc ggaaatctgc
     2581 gaagcgaaca ttctgagcac atttgaacaa ctgcaccaga ataaggatga ggtgtttgag
     2641 cgaggggtga tcaacgtctt caggggactg agctggaatt acaaaaccaa ttgcccctgc
     2701 aaatttggca gtaaaattat cgtcaacaac ctggtgaggt gggaccggtg ggggtttcat
     2761 ctgaacaacg gcccacaggc agatcgactt gccgatctgg aaagaatgct acacctgttc
     2821 agcggcaaac cgatccccga caaccgggaa aacatcacca ttcatctgga tgatcacatc
     2881 cagtctgttc agggtaaaga ggactatgaa gatgagatgt tcagcatcag atactttaag
     2941 aagggctccg cgcacatcac gttcaggaaa ccagagctgg ttgacaggct taatgatatt
     3001 attgccagac actacccgga agccctggca agcacagtga acaaaagccg caaggcataa
     3061 aagaggtatt ttacaattcg cgaatattaa ctattattaa ttgatagtaa tcaatcggcg
     3121 agaaaatcac catgcctacc agtgtatccc tcagtcctta ctttgaaacg tttatccgcg
     3181 aacaaataga aagtggtcgg tataacaata caagtgaagt cattcgtgcc ggacttcgtg
     3241 ctctggaaga acgggaacag cagataaagc ttgaatcact ccagagcgcc gtgaccgcag
     3301 gcatcaacag cggtgaaagt aaaagtgctg aagaggtatt cgggcgactg acgcacaaat
     3361 acaaaaagat ggcagaaggt gaacagccaa tatgaaactg actgtttcac cattagcaga
     3421 acacgatctt gaagctatag gtgactggat cgctcaggat aatcctgtcc gtgcaatcag
     3481 ttttaccgaa gagctttacc aacaatgtct gttgatagcc gaatcgccag tactctacag
     3541 agaaagaccc gagttagggg ctgggatcag aggttgttcg tatggtcgtt accttctgct
     3601 gttcagggta ctggatactg aagttcgtat cgaacgtatc gttcatggtt cccgggatat
     3661 caacagggta cttgcagagc cagacgacaa accatcagat catttctggc agctataaac
     3721 aaaatctgtg cgttaacgct ccctaacaca ggtattttat aaatctctgt attttgttgt
     3781 tacttcgatt ccgcgattca gcatatcgaa ccagacaaaa tgattacaac aaagcacaaa
     3841 aaagagggat tatcgcgtca aaagaattcg aattgcacat gggggcctca ttggtggccc
     3901 cgaaaagaac aaagacaaaa caatcgatat aaaacaattg attatataac cagtttcatt
     3961 ccgagtccgg gcaccaaatt catataaacg gacctccacg gaggtccgtt tttcgtttca
     4021 ggacgccacg atttaagcgt cctgcc
//
LOCUS       DAZFKM010000248         3958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFKM010000248 DAZFKM010000000
VERSION     DAZFKM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            178..1359
                     /locus_tag="ACHKAY_005080"
     CDS             178..1359
                     /locus_tag="ACHKAY_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO5703571.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQKPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEVLYGRDHVIYINTNILDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQ
                     E"
     gene            1373..1613
                     /locus_tag="ACHKAY_005081"
                     /pseudo
     CDS             1373..1613
                     /locus_tag="ACHKAY_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            1633..1964
                     /gene="sctI"
                     /locus_tag="ACHKAY_005082"
                     /pseudo
     CDS             1633..1964
                     /gene="sctI"
                     /locus_tag="ACHKAY_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1961..2695
                     /locus_tag="ACHKAY_005083"
     CDS             1961..2695
                     /locus_tag="ACHKAY_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO5703572.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            2711..3292
                     /locus_tag="ACHKAY_005084"
     CDS             2711..3292
                     /locus_tag="ACHKAY_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO5703573.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            3321..3506
                     /locus_tag="ACHKAY_005085"
     CDS             3321..3506
                     /locus_tag="ACHKAY_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703574.1"
                     /translation="MVSLKESILLETSAKIQSIEVKREEKFIQGYYDGYTKGIIDVMD
                     NFIPLISLLSSELEKKE"
     gene            3509..3940
                     /locus_tag="ACHKAY_005086"
     CDS             3509..3940
                     /locus_tag="ACHKAY_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703575.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLYIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
BASE COUNT         1347 a          611 c          739 g         1261 t
ORIGIN      
        1 aacattgagg gaatagggtt tataccttaa attgtaaata aagttatgca tagaggcatt
       61 taaaatagtt ctcggaatta taattttttt attctgtgtc atcataattt ggtcatgaaa
      121 tttccgtctt gttacactac tctaaactag agcttatgga attagcgggt ggtattcatg
      181 gaaaataatg ataaattctt atcacaagac ttattggaat cttatgccat tcgtttgttg
      241 agcggaccat tgaatggatg cgagtatgaa atacttaatg ggcgtctact tgttatcatt
      301 ggtaatgatg tttcgttagg aagaagtgat gctttttctg agttaccaga aaacacaata
      361 gtcgttcctt atggcgagct tacaggtagt tttgagataa taattactac cgaccctgat
      421 ctggtagtaa cattcagaga attaacagca caaaaacctg aagatagaac gttaacattg
      481 aatcagcaaa tagaagtttt aggtctcaaa tttgctgtaa aagaaaaaaa tgaagtttgg
      541 cagtattcat tgccaggcat tattgaaaat aacattattt ctacaaaaca acatttcttt
      601 agcagtaagc tgtttaagta tgtaatgctt ttttttcttt ttgctatcat tttcattgct
      661 ttttatattg ttaatgccag taatgatccg cagctgagac atatcgataa aattcttgta
      721 aacaaaaaca ggaattatga agttttatat ggtagagatc atgttatcta tattaatacc
      781 aatattttgg atgaagcagt ctgggtcaaa caagcactgg aaaaaaatca accaggaaag
      841 ccagtacggg tgataaatcc tgatgatgaa tcgatacgaa ttttttcatg gcttgctgat
      901 aatttccctg atttacaata ttttaaactt caactattag atgccagtaa tctaagattg
      961 acagtgagta agcaacggaa cgctatcaca cagcaactaa tcgataatct tattaaagga
     1021 ttactgcaaa ctatgccata tgccagcaat ataagtattg cggtattaga tgataatgta
     1081 ttggaaagtc aggctattga aacgttgtca gcgataggtc tttcttatga aaaatataaa
     1141 acagctaaca atgtgtattt caatatcatt ggtacgttaa gtgacagtga attaaataaa
     1201 attaataact atgttgacga atattataaa caatggggta aacaatatgt aagatttaat
     1261 gtgaatttga aaaatcagga cacaaataat agttcattta gctacggaga taaccgattc
     1321 gagaagtctc aaggtagcaa ctggacattt caggaataat tgaggaggac ttatggcaga
     1381 ttggaatggt tatattatgg atatcagcaa tcaatttgat cagggcgttg atgatctgaa
     1441 ccagcaagtt gaaaaagcgt tggaggattt agccaaccaa tccctccgac acgaaatttc
     1501 ttgccgaata tcaaagtgca ttagctgagt atacattata tcgaaatgcg caatctaacg
     1561 ttgtaaaagc gtataaagat cttgattcag caatcataca aaacttccgc taaactagat
     1621 aaggatttta ttatgttagt aagcaatatg ccacctattg atagagctga gcaatcaact
     1681 gctcatgaaa tacagcaagc gaaagtgatt gatttaaatg accgggttct caatcttgac
     1741 aacccggatg ataaaatgat ctccgctttt gccaactatg ctgtacaaac tgaaaattgg
     1801 cagcaaaatg cgttacaagc attaaggtct gataaagagg ggttaactcc tgaaaaatta
     1861 ttagtcttgc aggatcatgt gcttaattat aatgtcgagg tttcattggt ggaacattag
     1921 caagaaagat tgtcgctgca gttgagacgt tgacccgctc atgaaatata tatcattatt
     1981 attgtttatc ctgctgttgt gcggctgcaa gcagcaggaa ttattgaacc atcttgatca
     2041 acagcaagct aatgatgtcc ttgctgtttt acaaagacat aatattaatg ctgaaaagaa
     2101 ggatcaaggc aaaacaggat tttcaatatt tgtcgaacca acggattttg cttctgcggt
     2161 ggattggcta aaaatctaca atctaccggg taagcctgat attcaaatat cgcagatgtt
     2221 ccctgcagat gcactcgttt catctccacg agcggaaaaa gcaaggctat attctgctat
     2281 tgagcagcgg ttagagcaat ctctcaaaat tatggatggc attatttctt cgagagtaca
     2341 tgttagttac gatgttgata atggcgatag tggtaaaact gctttaccaa ttcatatttc
     2401 tgtacttgca gtatatgaaa aagatatcaa cccagaaata aaaataaatg atatcaagag
     2461 atttattgtt aatagctctg caagcgtaca atatgaaaat atttctgttg tattgtcaaa
     2521 acgaagagac attattgagc aagcaccaac atatgaaatt agcgaacctg tttttgctta
     2581 tgataaggct atgccggttt ctatcttact tgcattaata tccgtagcga cttgctggct
     2641 gttatggaag tatagggcca ttctaactaa tttagtcagg ttgaaaatta agtagacata
     2701 tgagagcgtt atgaatcttg ccctaagaaa gattatatat gatccaatta gttatattca
     2761 tcctcaacga gtaagcttga ataatacacc tattaacaat ccggtgctta gatctattac
     2821 caatgaaatg attgttctgc agtacaatct ttcagttgaa cattttaatc tgaatagttc
     2881 acttatttac tacataaaca attggaattt atttccattg ttctgtttat tcagtggtta
     2941 tcacttttat cgagaaaggt ttgcagaacg cggatttttt tataaggttc ctgctgtgct
     3001 aagggattat ttatcagcta taccggtgaa aataaatgaa aaggcacgat acaaacccgg
     3061 catcgctagc tatcacaata tcatcacatg tggtttttca acgttgtcac cctatataag
     3121 gcaacaacca ttggcaatgc agcaacgatt taatttatta tttcctgatt ttgttgatca
     3181 catacaattg ccgttaccac ttgcatcaac tcttttagag agaattacat tttatgcgaa
     3241 aaaaaataga gatgagcttg ataaaatctc ctgcaaatgg tgttgtgatt aaaagaaaaa
     3301 tttctgatgg attgaaataa atagtttctc ttaaagaaag tatccttctt gaaaccagcg
     3361 caaaaattca gagcattgag gtaaagagag aagaaaaatt tattcaaggt tattatgatg
     3421 gctatacaaa aggcattatt gatgtgatgg ataattttat acccttgata agcttgttga
     3481 gctcagaact agaaaaaaaa gaataaacat gataaacgat ctgaagagta tattgttaaa
     3541 atcctcagag gaagttgatg tttttatcaa aatttttgaa agttggagaa aaaaattacc
     3601 ggcaatatca ggtcctgtaa atctctatat acctaccaga ttcaaagata agtatcttga
     3661 agtagagtca tattttgtcg acaaatcaat atggaatgtc catattacat ttcatgatga
     3721 taagcgtttt gtgtttttta ccgatcaatt tattgctgaa tttagtccgc aggaatttgt
     3781 ggataattgt gagcaatatt taattaataa tcattgtttc tcccctgaca aggttaacga
     3841 aatctgcgaa caagccagac attatttagt tgaaaaaatg tttgaaacac attctttgga
     3901 tatgaataat tctgttttgg caagccctga ggacttatag ctccctggct caagttta
//
LOCUS       DAZFKM010000249         3952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFKM010000249 DAZFKM010000000
VERSION     DAZFKM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..685)
                     /gene="traJ"
                     /locus_tag="ACHKAY_005087"
     CDS             complement(14..685)
                     /gene="traJ"
                     /locus_tag="ACHKAY_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFO5703576.1"
                     /translation="MYPTDPRQLNTERQIYLDKQFFVDVFSIPACVRNTNGDFIGYNE
                     KFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLPSSMNKIQSVAIGDKLWLVQF
                     IPLIYGEVVNVLWLFFCKNSNVIVDYCRGLRTNITNDRMLEFKNKSTEIQWKVFILYS
                     FGFCHESIASLLSITNGSSRNAISEVYKFFGIHSKHDLLMIFHTSRMHSLFFDELFFI
                     LKCAE"
     gene            complement(879..1262)
                     /gene="traM"
                     /locus_tag="ACHKAY_005088"
     CDS             complement(879..1262)
                     /gene="traM"
                     /locus_tag="ACHKAY_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO5703577.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            1585..2187
                     /locus_tag="ACHKAY_005089"
     CDS             1585..2187
                     /locus_tag="ACHKAY_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO5703578.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQNN"
     gene            complement(2484..3305)
                     /locus_tag="ACHKAY_005090"
     CDS             complement(2484..3305)
                     /locus_tag="ACHKAY_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5703579.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEKFREWKS"
     gene            complement(3415..3711)
                     /locus_tag="ACHKAY_005091"
     CDS             complement(3415..3711)
                     /locus_tag="ACHKAY_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703580.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(3735..>3952)
                     /locus_tag="ACHKAY_005092"
     CDS             complement(3735..>3952)
                     /locus_tag="ACHKAY_005092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5703581.1"
                     /translation="SRVNEQPTKFFTQNPVKNFAFCPGQVRQAVAVMPLAAKVLKQVM
                     VNTGGNKRQVPAKRQNRGSRNSTDDGG"
BASE COUNT         1190 a          866 c          751 g         1145 t
ORIGIN      
        1 aacgaaacat caactattca gcacatttta aaataaaaaa caattcatca aagaataaac
       61 tatgcatcct gcttgtatga aaaatcatta acaaatcatg ttttgaatgt ataccgaaaa
      121 acttatacac ttctgaaatt gcattccggg aagaaccatt agtaatagac aaaagagatg
      181 ctatcgattc atgacaaaaa ccaaatgaat aaagaataaa aactttccat tggatttccg
      241 ttgatttgtt tttaaattca agcattctgt catttgttat atttgtacgt aatcctctac
      301 aataatccac tatcacatta gaatttttgc agaaaaaaag ccataaaaca ttaaccacct
      361 ccccatatat taatggtata aattgtacca gccagagttt atcacctata gccacgctct
      421 gtattttatt catggacgaa ggcaaagaca tagcatccag ttcttctctt aagaacgatg
      481 tagctacttg tacaggaagg gagtaaaacc actctttgat atcaagacta cctataaatt
      541 ctttagagaa tttttcattg tagccgataa aatctccatt agtatttcga acacaagcag
      601 ggattgaaaa aacatcgaca aaaaactgtt tatccaaata tatttgacgt tcagtattaa
      661 gttgtctagg atccgtcgga tacataggaa cctcctctca aaggattcta tggacagtcg
      721 atgcggggag gtcgctctcc ctgcatcggc gatttttaat atctgtttta acgtggcatt
      781 aaccacataa ttaatataac ctttatgtgg ttaattgtca tcaaattgaa ccagatcaaa
      841 atcctgttat ttgaagtaaa tcaactttta attctttttt attcctcatc attttttgga
      901 aaaaatcgtt ccatctcaga tgacaccttc tcacgaatat cttcaaccat attggcatat
      961 tcaaactttg gattcccgga gacatgggga cttagagact caatacccaa aattttcgct
     1021 actgatgact gtgtttttac aacacattca agaagaagtt tattaaactc tgtctgatta
     1081 aacgcagact ctttacgctc catctgagcc tcatatacac gaagccccag ttcaagaagc
     1141 attgaagctg ttcccgaaac acttatatct ttatctcttg ccccctcctg acgacgctgt
     1201 tcaactatcg catttacttt atcatagaca tcattactga tatacaaaat tactctggcc
     1261 ataagataaa acctttcatt attaagcaat gaacttttca ctataaatat gcatatagtg
     1321 tttacaagta agaaagacac tcctagcagc gcctctagga tcatcctata aaaaaatgcg
     1381 atccggcgct aggggcgtcc ctaatatata tcaatgtttt tcatgaaaat tgtcagtact
     1441 gatcctaata agagtcgcta tagggtcgta acaggatcgc caacgactct ctatttaata
     1501 attcagaatt attaaatata aatagcgttt gttaattaca tgatttaaaa cgtaaatcag
     1561 caaaaacttg tttttgcgta gtgtgtggtg cttttggtgg tgagaaccac caacctgttg
     1621 agcctttttg tggagtgggt taaattattt acggataaag tcaccagagg tggaaaaatg
     1681 aaaaaatgga tgttagcaat ctgcctgatg tttataaatg ggatctgcga agccgccgat
     1741 tgctttgatc ttgcaggtcg ggattacaaa atagacccgg atttactgag agcgatatca
     1801 tggaaagaat cccgttaccg ggttaatgcc attggtatta atccggtaac gggatatggc
     1861 agcggactga tgcaggtaga ttcccagcat tttaacgaac tggcccgcta tggaattaag
     1921 ccggaacatc tgacaacaga tccctgcatg aacatttata ccggtgctta ttatctggca
     1981 atagccttta aaaaatgggg agtctcctgg gaggccgttg gtgcatacaa tgccggattc
     2041 aggaagaccg aacgccagaa ccagagacgt cttgcctacg catcagaggt ttaccggatt
     2101 tatacctgga taaagagcag taaaggcatc cgggttccga ccacgaagaa atcactttcc
     2161 caaattaaca gtgtgcagaa caattaatat tgtatacatt gtcaaatacc ggttatgccg
     2221 cgacagcggc atggaaggcc acacacaaaa atatcatatg agtgataatg ctgactggcc
     2281 gaagccccgc aaagcgggac cccatcttat atatatccgg taactgcgca gcctcctgcc
     2341 agaccatttt tcgcgggcac aatatttaca gactaccgaa aagccagttg atgaaattga
     2401 ggcctatcag acgacacagc aatgccgcag gatgataata taagtaaagc cccgaaaaat
     2461 ttttcggggc tttacttata ttatcatgac ttccactccc tgaacttttc ggcaatcatc
     2521 cataatgctt tgttgattcg gatatcaccg tcaatgcctg taatggctct tgttctggta
     2581 tttttaccgg aggcacttcg ccccgataat ccacctttta tcatattctc ctgaacccgc
     2641 tgccaggttg tccagagatc gttctgttta tcctcccaac ggcggggagt aattatttgt
     2701 tctggcgtca ctggcgtttt attttcatct tcgtacctgg ccatcaatgc agctctgcca
     2761 aataaatgtt gttcgtcact gttaagatgt atctctttca ttgcctccat attttcagtg
     2821 actttatcaa agacaccgag cacttcatac gctccctcaa taacctgccc gacaatatca
     2881 cctttatgtg gaacgcggat ttcgccaaaa ttattcccac acactaggcc atttgtgcag
     2941 acaaaacgaa aaattcctgg gatcatctga taactggatg aaccatcatg tgaattaagc
     3001 aggataattt caggaacttc tttcccgtta atatgccctt ccctgcgaag acgtaacata
     3061 tgtttactgt attcgcggcg tcctaaatca cgaacccgac tctgacaggc aaagaacggc
     3121 tggaaacctt catcacgtaa cttattgatg atattgatgg ttgggatata tgtataacgc
     3181 tcactccggg attcatgttt atcaccagaa aagacagatg gaacaaactg cattaattcg
     3241 tcatccgtta acgggcgctc acggcgaata gaattatatc gaccaaaacg ggaagctaat
     3301 cgcataacag taactccttt atgaaggcat aaaattaata aaagtttttt cgcttaatat
     3361 catccccctc ggttatcgtt aacaaaaaga caacaattcc gaggggaagg accatcaaat
     3421 caggtcagtt cgggactgac caacctgccg agtgaaaagc agtaacagcg cgatcaactc
     3481 atcagcgttt tttctgacaa gaggagtatg attctccagg ctatccagta agtcatgcag
     3541 ctcattattg acctggtgga gcaccttaaa gagtgcacca gaatcccggc agctctgata
     3601 tgcaagattt gcctgaacag cacgattctg catgatcccg atttgtgcga ttgcgtcttt
     3661 ctgctgatcg cctgttggct gataagtggt gttaaaagaa aatgcgctca taattcctcc
     3721 tgtatctgat gttttcagcc cccatcatct gttgagtttc gggagccgcg gttttgacgt
     3781 tttgcgggga cctgcctttt gttccctccg gtattcacca tcacctgttt gagaaccttc
     3841 gccgcaaggg gcatcactgc cacggcctga cgcacctgtc ctgggcagaa cgcaaaattt
     3901 tttaccgggt tttgggtgaa aaattttgtg ggctgttcat ttaccctgga ca
//
LOCUS       DAZFKM010000250         3898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFKM010000250 DAZFKM010000000
VERSION     DAZFKM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..62
                     /locus_tag="ACHKAY_005093"
                     /pseudo
     CDS             1..62
                     /locus_tag="ACHKAY_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            66..257
                     /locus_tag="ACHKAY_005094"
                     /pseudo
     CDS             66..257
                     /locus_tag="ACHKAY_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(265..618)
                     /gene="cmi"
                     /locus_tag="ACHKAY_005095"
     CDS             complement(265..618)
                     /gene="cmi"
                     /locus_tag="ACHKAY_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO5703582.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYHCEEGYVEYAND"
     gene            complement(791..1573)
                     /locus_tag="ACHKAY_005096"
     CDS             complement(791..1573)
                     /locus_tag="ACHKAY_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO5703583.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNKHA"
     gene            complement(1575..1988)
                     /locus_tag="ACHKAY_005097"
     CDS             complement(1575..1988)
                     /locus_tag="ACHKAY_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703584.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELIFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2548..2778
                     /gene="vapB"
                     /locus_tag="ACHKAY_005098"
     CDS             2548..2778
                     /gene="vapB"
                     /locus_tag="ACHKAY_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFO5703585.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLEYEKADPDFMAEREDVVSDEGRFNL"
     gene            2775..3191
                     /locus_tag="ACHKAY_005099"
     CDS             2775..3191
                     /locus_tag="ACHKAY_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO5703586.1"
                     /translation="MNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDKFCARLDAILPWDRAAVDATTKIKVALRLAGTPIGPN
                     DTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK"
     gene            complement(3353..>3898)
                     /locus_tag="ACHKAY_005100"
     CDS             complement(3353..>3898)
                     /locus_tag="ACHKAY_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703587.1"
                     /translation="MQICKSITASADCEEIIKLIYLRRYFEIVDERGDAYQLLSNLFH
                     RRVAPLDYREPAAAGSGYPKMAPEKIQQALRDIREYVDSFDYPRLQALVSSPDEIKNL
                     YRRCRNGYEKLQVFRLLELDQDHPVIRKFVNETYHIENEFICQLDPSRFDLIPEYVIM
                     ECDKLIALPPAANQSSVARIA"
BASE COUNT          980 a         1004 c          963 g          951 t
ORIGIN      
        1 ttattatcta tcaacaacag aatgagcaat tatatccagc acaggaacat cttcttttac
       61 aatgttcata tgatgataaa tacgatttta acgccagcac tcaccgtggc atcatcggag
      121 agtcgctcac aaggctcggg gcgatgtttt ctggtaaaga gtaccagata ctgcttcctg
      181 gtgaaattca cattaaagaa agtggtaagc gagaatataa gggccgggat tccggccttt
      241 ttatgcagcc tttataacag tgtattagtc attcgcatat tcaacatatc cctcctcaca
      301 atggtatatg ccataataca ttctcttacc ctccgggcct tcagctttaa atgggacaag
      361 aagtgttttt tcatcttcaa catctgttaa acttctctca atcttttccc atgtaatttt
      421 cgggatttct gtaccaagaa gttgaacatc gttgtcccag cgtttcattc tttgcaggta
      481 atcatttttt acaaacgttc tggcctgttc atcgctgacc tgatagcagg ccggaccttt
      541 atcttcagac cagaaaaaaa cagcagcaag agcaataatc gttaaaataa aaataaattt
      601 catgctaatt actttcataa aatctccaca acttctgtcg ttcattcgat ataaaaaaac
      661 attacgaata tatccgtaca atgtgagcat actgtgaagt ggctttaaca attatgaaat
      721 aatacaagag atcacatatt gcagtggtct cttttttata gaagaaatca caattcataa
      781 ttgtgactta ttatgcatgt ttgtttttca gcattgtgac cgcatcggac tcaggcatcg
      841 aaaactgcac ccggtgccgg gcagcgacat ccagcgcaaa caccttcgtg tagacctccg
      901 ttgagctgat ggacttatgc cccatcagac tctgcaggac cttcagcggt ataccggcat
      961 acagcatatg catcgcatag gaatggcgga acgtatgtgg cgtgaccggg acagagaacg
     1021 tcacaccatc agcggcagcg gcggcaaccg cctccccaat ccaggtcctg accgttctgt
     1081 ccgtcacttc ccagatccgc gctttctctg tcctgcctgt tcgtttatta cgccgttcca
     1141 tggggatttt caatgtggcc accatcgtct gcagctggct gacgtaccag gagtcggaga
     1201 gcggaaccag ccggtgagtc tgctgcccgg cgggcgttct tcctgccgtc ctggcggctt
     1261 tttcggtccg ttgtttcagg gtcgcaagct gcacaaacgg atacggaggc gtgagcgaaa
     1321 aatccccccg cgtcagcgcc agtgcttcat taatgcgcgc accggtgttc cacagtgtgg
     1381 ccagcagcat cttgcggcgc agatccggga cgtaatggag cagggcgctc acttcagggg
     1441 ccagcaggta tttgggcagt tcatcatgaa ccatcgacat ctggcggagt gccagcgctg
     1501 ccggataatc gatggcaaca ggaagctgag cggcatcccg ggcatggtgg atcggtgccg
     1561 gcaggtgctg catatcagtg ttcactccac ggggaaacgt cgggcaggtt ttccacaacc
     1621 cggtactttc ccggataacg gagctggtta ccgccacgcg tccagacggt gacaacaaaa
     1681 ccccctccct ggttgttttc ggggagcagg cgatcgcact gctcactcag catgtggatc
     1741 acgaacggcg catcgctgcc gtcttcaaat atcagttcca gcgcatcgcg gccatgcagc
     1801 gttcccttac tgatgatgac gtactcagcc ccccgcatct ccctgagtaa atgttttgcc
     1861 gcatccggga caagcagcct ggctgcgccg gcattccagc tgaggtagac atatccggcc
     1921 tgcgcctgta cagactgcca gtaatccgtt ctggcgacag cctgaccttc attctcaata
     1981 aaaaacatgg gtgactcctt ttttcttttc cgggcaggga cggtcatgcg ataacgtcat
     2041 ccatcatcac tgctcttacc gtcacgccat gtgataacat catgccgtta tcatcatcac
     2101 catgataaca agcatccata taaccacgct gttaaattac cggaggacca tttccgacag
     2161 atccgatttt tctgttattg acgggttttc ggaagtgtat cacgctgagg aaataatcat
     2221 atcgctgttt tccgcccctg cgcgacgcat cacaggtcag ggagatgaca caacatatag
     2281 tgtcatcagc agatgaatcc ggcaccacat gttgtgatga cgggaggtgg cgttgtgtgg
     2341 ggggagagga agatcctgaa caaccttttt tcggatctcc gataaaaaag aaagaaatag
     2401 agatatgcta aacatatgcc tggcatctac atatgctacc gctatacata tgccacacat
     2461 ctgcctatac taaacatatg cacacacccg gtgctttgaa tattccggat atgtttcaca
     2521 tatccatatg ctcgggagag aaagaacatg agaaccgtat cgatatttaa aaatggcaac
     2581 aaccgcgcca tccgtctgcc tcgtgatctg gattttgagg gggtgagcga gctcgagatc
     2641 gtccgggaag gggacagcat catcctgcgc cccgtccggc caacctgggg atcattccta
     2701 gagtacgaaa aagccgatcc agattttatg gcggagcgcg aggatgttgt cagcgacgaa
     2761 gggcgattta acctgtgaac aagacttata tgcttgatac ctgtatctgc tcgttcatca
     2821 tgcgtgaaca gccagaagcc gtcctgaagc gtctggagca ggcggtgcta cgcggccacc
     2881 gcatagtggt ctcggccatc acctactctg aaatgcgctt cggggctacc ggcccaaagg
     2941 cctcaccacg tcacgtccaa ctggtcgata aattctgcgc ccgcctcgat gccatcctgc
     3001 cctgggatcg cgccgcagta gacgccacca cgaagattaa agtggcactg cggctggccg
     3061 ggacgccgat cggcccgaac gacacggcga ttgccgggca cgccatcgcc gccggggcga
     3121 tactggtaac gaataataca agagaatttg agcgggtgcc tgacctggtg ctggaagact
     3181 gggtgaagta agacactctt ttcaaagtca ggagataagg gcaccaagaa acagcccgta
     3241 tacgggctgt tttaacagat atctacagta agcggtccta aactgtaggg tatcttcgaa
     3301 gatcagacta tcagggagct aaagcctatc ttccggcggc aaaaacagtg gctcaagcga
     3361 tacgggcaac cgagctctgg tttgctgccg gtggcagggc gatgagcttg tcacattcca
     3421 taatcacgta ctccgggatg agatcgaatc tggacggatc tagttgacag atgaactcat
     3481 tttcaatgtg gtacgtttcg ttaacgaatt ttcgtattac cggatggtcc tgatccagtt
     3541 ccagtaaacg gaatacctgc agtttttcat agccgttacg acagcgacgg tacaggtttt
     3601 ttatttcatc tgggctgctg acgagagcct gaagtcgcgg gtaatcaaaa ctgtccacat
     3661 actcccggat atcccgcaag gcctgctgaa ttttctcagg agccattttg gggtagcccg
     3721 agcccgcagc ggcaggctcg cgataatcca gcggggcgac gcgtcgatgg aatagattgg
     3781 atagaagctg ataagcatca ccacgttcgt caacgatttc aaagtagcga cgaagataga
     3841 tgagcttaat gatctcctca cagtctgctg acgcagtgat ggatttgcag atctgcat
//
LOCUS       DAZFKM010000251         3895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFKM010000251 DAZFKM010000000
VERSION     DAZFKM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..3072
                     /gene="agn43"
                     /locus_tag="ACHKAY_005101"
                     /gene_synonym="flu"
     CDS             <1..3072
                     /gene="agn43"
                     /locus_tag="ACHKAY_005101"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO5703588.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSR
                     AGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLE
                     TGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDM
                     TFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSP
                     SQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            3193..>3895
                     /locus_tag="ACHKAY_005102"
     CDS             3193..>3895
                     /locus_tag="ACHKAY_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFO5703589.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLT"
BASE COUNT          899 a         1054 c         1165 g          777 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggattcgtc
     2281 cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac cgcgggggta
     2341 tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc ccgtgccggc
     2401 acggtccggg atgatgccgg cagcctgggc ggatacctga atctgataca caacgcctcc
     2461 ggactgtggg ctgacattgt ggccctggga acccgccaca gcatgaaagc gtcaacggac
     2521 aataacgact tccgcgcccg gggttggggc tggctgggtt cactggaaac cggtctgccc
     2581 ttcagtatca ctgacaacct gatgctggag ccacaactgc agtatacctg gcagggactt
     2641 tccctggatg acggtaagga caacgccggt tatgtgaagt tcgggcatgg cagtgcacaa
     2701 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg cgaaggcacc
     2761 tcatcccgtg cccccctgcg tgacagtgca aaacacagtg tgagtgaatt accggtgaac
     2821 tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggagatat gcgtgtgggg
     2881 acttccactg caggcagcgg gatgacgttc tctccctcac agaatggcac atcactggac
     2941 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt
     3001 tatgcccaca gcgtcagcgg cagcagcgct gaagggtata acggtcaggc cacactgaat
     3061 gtgaccttct gacagaacca tcgcctctct gtggtcccgg tcatcatgac cgggacccgg
     3121 accggcgcaa cggatcttca acgccacatt cgctggcatt aacaataaca tgatattcat
     3181 cacggagtga ctatgttaca gatagtcggc gcgctgatcc tgctgatcgc aggatttgcc
     3241 attcttcgcc ttttgttcag agcattaatc agcacggctt ctgcgctggc agggctcata
     3301 ttgctgtgtc tgttcggccc ggccttactg gctggctata tcaccgaacg cataacccgg
     3361 ttgttccata ttcgctggct ggcaggcgta tttctgacga ttgccggaat gatcatcagc
     3421 ttcatgtggg gacttgatgg taaacatatc gcgctggagg ctcacacctt tgactctgtg
     3481 aaatttattc tgaccaccgc tctcgccggt ggtctgctgg ctgttcccct gcagatcaaa
     3541 aacattcagc agaacgggat cacaccagaa gatatcagca aggaaattaa cgggtattac
     3601 tgctgttttt atactgcctt tttccttatg gcgtgttctg catgcgcacc attgatcgcg
     3661 ttacagtacg atatttcacc gtcactgatg tggtggggcg ggttgttgta ctggctggct
     3721 gcattagtga cgctgctatg ggcggccagc cagatccagg cgctgaaaaa actgacctgt
     3781 gccatcagcc agacactgga agaacaaccg gtgctcaaca gtaaatcgtg gctgaccagt
     3841 ttgcaaaacg attacagcct tcctgactca ctgacggagc gcatctggct gacgc
//
LOCUS       DAZFKM010000252         3864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFKM010000252 DAZFKM010000000
VERSION     DAZFKM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..69
                     /locus_tag="ACHKAY_005103"
     CDS             <1..69
                     /locus_tag="ACHKAY_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HFO5703590.1"
                     /translation="YAEVLIAITEYHRALLSRLMAD"
     gene            319..657
                     /locus_tag="ACHKAY_005104"
     CDS             319..657
                     /locus_tag="ACHKAY_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703591.1"
                     /translation="MNISIKNNYLKTIARFDGKTLRDAFSRKIATFDGKILRDASLKK
                     IATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGQNLRDASSKIILSADAEVGI
                     VVMAFVAELI"
     gene            1231..2370
                     /locus_tag="ACHKAY_005105"
     CDS             1231..2370
                     /locus_tag="ACHKAY_005105"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFO5703592.1"
                     /translation="MNKPRFNGTPVASLDSLSAMLGIERKRLDWIVKSVSMSYKQFKV
                     ETGKNKKERQIFEPKRSLKGIQKKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRK
                     RTVICLDITNFYPSISKKDVCSIFKNLMRFSPEVSLCLTELVTLNNKVPQGGCCSSYI
                     ANLLFFNSEYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYR
                     LSINESKTTIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERH
                     TKEYHDLWNRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNK
                     FTPPLTDEQIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
     gene            2375..2578
                     /locus_tag="ACHKAY_005106"
     CDS             2375..2578
                     /locus_tag="ACHKAY_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="HFO5703593.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICK
                     GILVRFKLEKTDKGFKAVAIQKV"
     gene            complement(3415..3609)
                     /locus_tag="ACHKAY_005107"
     CDS             complement(3415..3609)
                     /locus_tag="ACHKAY_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5703594.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
BASE COUNT         1061 a          721 c          902 g         1180 t
ORIGIN      
        1 tatgcggagg tgctgatcgc tattacggag tatcaccgtg ccctgttatc caggcttatg
       61 gcagattagc ttcccggaga gaaaactgtc gaaaacagac ggtatgaaca ccgtaagctc
      121 ccaaagtgat caccattcgc tttcatgcat agctatgcag cgagctgaaa acgatcctga
      181 cgcatccttc ctgtttttcc ggggtaaaac atctcttttt gcggtgtctg cgtcagaatc
      241 gcgttcagcg cgtttcagtg gtgcgtacaa ttaagggatt atggtaaata caattggtta
      301 aattactatt ggtgataaat gaatatttca ataaaaaaca attacttaaa aactattgct
      361 cggtttgacg gtaaaacctt aagagatgcc ttttcgagaa aaatagcgac ttttgatggc
      421 aagattttaa gggatgctag cttaaaaaaa atagcgactt ttgatggcaa ggttttaagg
      481 gatgcctctt taaaaaaagt ggcgactttt gatggcaagg ttttaaggga tgcctcttta
      541 aaaaaagtag caacctttga cggtcagaat ttaagagatg ctagttcaaa aattatcctg
      601 agtgccgatg ctgaggttgg gattgttgtt atggcctttg tagctgagtt gatttgaatc
      661 tcatgctgca taccttttag cctatcgctg gcaggataag gcgctttctt gttggtgaca
      721 gaaagataaa aaactttata ataatcagcg aagcgggcga accacgcctg gctctcgctc
      781 tttaacaaat ggttacgtgt atgttgcgca aggaaaccga tgccttgttt ttaaggcgat
      841 ctcgcatgag atcatgaccg gcctggccgc taattaatgg tgagaacgcc cctgagcgtg
      901 tatcagcact aaaagaatag cgacgattgt ctggcaatgt gagttaaaag tcccaaagag
      961 ccaatagagt ttttccttgc tttcctactg gtctggcaaa atttcataac caataggagt
     1021 tgcatcatgt ttattatcaa catcaacatt aacgtaacca agagctcttc atcttgttct
     1081 ggagacggtc agatgaagaa aaaaagagag gttggctctc tggaggcact gaaaaaaaca
     1141 cagtttttgt tttctatgtg cttgattatg tttaaagtaa tagattacgt atcgaaactt
     1201 tttaactaat cgacttttaa ctcaaacatc atgaataaac ccaggtttaa tggaactcct
     1261 gttgcatctt tggattcttt atctgcgatg ttaggcatag aaagaaaaag attggactgg
     1321 atagtcaagt ctgtatcgat gtcgtacaag cagtttaagg ttgaaacagg gaagaataaa
     1381 aaagaaaggc aaatttttga acccaagaga tctttgaaag gaattcaaaa aaaaataaat
     1441 aaagaaattt ttgagaaaat tgattaccct cattatttgc atggggcgtt aagtggtcgc
     1501 gattatatat ctaatgccgc tgttcatact aggaaaagaa cagttatatg tcttgatatc
     1561 accaattttt atcccagcat tagtaaaaaa gatgtttgtt ctatttttaa aaatttaatg
     1621 cgtttttctc ctgaggtttc tttgtgctta acggaacttg tgacgttgaa taataaggtt
     1681 cctcagggtg ggtgctgttc tagttatatt gcaaatcttt tattttttaa tagtgagtat
     1741 aatctctata atagattgaa gtctatgggg ctttcatatt caaggctttt agatgatatt
     1801 actatatcct ctgataagga tctttcatca gaggaaaaaa caaaagtaat aaaactcgtt
     1861 cacggaatgg tgaatcagta cagactttca ataaatgaaa gtaagacaac gatagagcac
     1921 tcaaaagatt cgtcttctaa acttagtgtc actgggttgt gggtaaagca tggcgttcct
     1981 aagttaacaa aagaaaatag acgttatatc aggtacttag tttatatatg taaaaaacaa
     2041 ggtgcttatg aacgtcatac gaaagagtac catgatttat ggaatagatg ctctggcaag
     2101 gttgctcaaa tgtcgaggct tgggcatgtc caggcggttg aattgagggc gattctttct
     2161 gagataatgc cagtttatga tgattacaag atatctaaat taaagttgat ggctaagcat
     2221 tatctaaata aatttacgcc gcctttaact gatgagcaga taaggaaaat agataggatg
     2281 ctctatgatt ttgatatagt agggcggact aataagaatc ttgcaaaact atatagacgt
     2341 aagcttgtgg cattacttcc agataggtga ttatatggaa aatggatttg ttaattttta
     2401 tgaccatgtt aaagggtatg gctttataag gcgtgagcgt ggacgtgatg ttttctttcg
     2461 gtatgatgat tttttgtttt tagggcatga tgtcgatata tgtaaaggta ttttagttag
     2521 gtttaaatta gaaaaaacag ataaagggtt taaagctgta gcgattcaaa aggtataaca
     2581 tcgtggatgt gtcagcttgt ttttatgata tgagaacttg agatcctttc tatctcgtcg
     2641 taatctcttg ctgtgtaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctca
     2701 gagctaccaa ctctttgagc cgaggtaact ggcttggagg agcgcagtca ccaaaacttg
     2761 ttctttcagt ttagccttaa caggcgcatg acttcaaggc taactcctct aattcggtta
     2821 ccagtggctg ctgccagtgg cgataagtcg tgtcttctcg gtttggattc aagtcgatag
     2881 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     2941 gagcgaacga cctacaccgg gccgagatac caacagcgtg agctatgaga aagcgccacg
     3001 tttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3121 cacctctgac ttgagcgtcg gtttttgtga tgctcgtcag gggggcggag cctatggaaa
     3181 aacgcctcct gctcgccctt cttctgggcc tttgtctttt gctcacatgt tctttccggc
     3241 tttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagttg acaccgctcg
     3301 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgccttat
     3361 gtgacatttt ctccttacgc tcctctatgc cgttctgcat cctgtcctga tgcgttatat
     3421 cccggtgcga ttttccgctt caaagcgtgt ctgtatgctg ttctgaagtt cttccgcgag
     3481 ttcgtgcagc ttctcacaca tggctgcctg ttcgtcagca tcaagtgcgt ccagtttttc
     3541 gagcagcgtc aggctctggc tttttatgaa tcctgccatg ttgagtacgg tttgctgctg
     3601 cttgttcatc tttctgtttt ctccgttctt tctgtcatct gctttgtgtg attatatcgc
     3661 gtaccacttt tcgactgttt taccgtcggt attctgctgt ctgactcttt gacgggcatt
     3721 tctgtcagac aacactgtca ctgccaaaaa actgccgtgc ctttgtcggt aattcgagct
     3781 tgctgacagg acaggatgtg taattgttat accgcgcatg catgcacgct attacaatta
     3841 ccctggtcag ggctttgccc cgac
//
LOCUS       DAZFKM010000253         3841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFKM010000253 DAZFKM010000000
VERSION     DAZFKM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..288)
                     /locus_tag="ACHKAY_005108"
     CDS             complement(<1..288)
                     /locus_tag="ACHKAY_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5703595.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDM"
     gene            complement(427..660)
                     /locus_tag="ACHKAY_005109"
     CDS             complement(427..660)
                     /locus_tag="ACHKAY_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703596.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            774..1478
                     /locus_tag="ACHKAY_005110"
     CDS             774..1478
                     /locus_tag="ACHKAY_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703597.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1615..1902
                     /locus_tag="ACHKAY_005111"
     CDS             1615..1902
                     /locus_tag="ACHKAY_005111"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO5703598.1"
                     /translation="MKVLNVEKLHSFSRKHNQAKGALDSWYDEVIRENWKTTQDIRNR
                     FNSADFLPNNRVIFNIKGNNYRLVVQVVYQAGMVIVERVGTHAEYDKWRLK"
     gene            1899..2555
                     /locus_tag="ACHKAY_005112"
     CDS             1899..2555
                     /locus_tag="ACHKAY_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFO5703599.1"
                     /translation="MNRTSWRIIKNSEEHAAAMERLIELASSDLQPGTEDFDEFELLG
                     LLIEHYESREFPMDKPDPIEAIKFRMDQQGLSYADMKQYIGSASKVSEVLNRKRPLSL
                     SMIRRLHDGLGIPADILIQDMSAIEWSLVDAEEEETAMTSVIARCESAVTSPSAYFAE
                     KATESYFSKMLFSAVRGNGKCKEKRNVFSLISNLSSSFTAASNLNDEMTSDGNYLLLP
                     "
     gene            2552..2956
                     /locus_tag="ACHKAY_005113"
     CDS             2552..2956
                     /locus_tag="ACHKAY_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703600.1"
                     /translation="MKIELISKKVERLVMTRLEGDSTAKKAIKTTVNLNNELYTNVKD
                     SKLFRVRYFASVTIEGRLEMDITYDFDFRSEDDFSHEMAKSYEVRSIAPNMAYPYIKT
                     YAEQIILMSNLGRFTLPYFDFLANPMETNSNK"
     gene            3743..3811
                     /locus_tag="ACHKAY_005114"
                     /pseudo
     CDS             3743..3811
                     /locus_tag="ACHKAY_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT         1181 a          790 c          848 g         1022 t
ORIGIN      
        1 catatcaatt tgcttagagt ccccggcggc caccggggat tttttctttg tgatttcatc
       61 aagcgcatac ttaaaagctc tgctaatcgg actgatgtct gatgccatgc caaaagcaca
      121 caagaccgaa gctataaacc tccagtctgt tctgcttatc ttcgattcat gacagccaat
      181 catctttgcc agaccgcgct gggtaagcgt tgacaggttg atgagtaaat cagtttcagc
      241 gcgatcaatt tctcgctgtg atagtttgct gtaacttgtt tgttccattt cttaagattt
      301 ccaatagtga atagctagtt gaaaggtatg cgtggaaacg catatggcct tagttggtca
      361 gatatattgg gactcgcttt gtcagcgacg taggacgaat gtccattgtg aaaatagcgg
      421 tgttacttat gcagccgatg ctctacgcga tacgaacact aggttttcct ttttcacagg
      481 tttataaccc gtgaaattac gagtagcttc ttcgattgca ttcgctttat caggggaagc
      541 tcttcgaaat ccatatgcaa tctggtcaag atagccaact gaagttttcg ctaatgcggc
      601 gagtcgcttc cattcctcac tagaagcctc ttttcgccag cgtagtagtt cattactcat
      661 tagtgcctcc gtttatcaca cagaacaact ttaccatttt gataaatcat ccgcaatgta
      721 aatttatcat attgcgtatt tatccatttg ctaaatagag ggaaaattgt gagatggaaa
      781 acaaagatat tcgcaaatcg aatctggcgt ttttgctaga tgagcataaa aaaatcgcgg
      841 gtaacactaa tgcaagcttt gccgataagc ttggggttag cccttctcaa ctcacgcaag
      901 tctccggtga aaaaagcact cgaaacatag gggataaact agcaagaaaa tttgaagccg
      961 cgcttgggtt acctaatggg tggcttgatt tggtacatga tgtaacacca attgcatcat
     1021 gctcagattc tttaactttt gtcggtcagg taagaaaagg gttagtgcgc gtggttggtg
     1081 aggcaattct tggtgttgat ggtgccatcg agatgaccga agagcgcgat gggtggctca
     1141 aaatttatag cgatgatcca gatgcctttg gtcttcgtgt gaaaggagac agcatgtggc
     1201 ccagaataaa atcaggagaa tatgtactca ttgagcctaa caccaaagta ttcccgggtg
     1261 atgaggtgtt tgtcagaact gttgaaggac acaacatgat caaagttctt ggctatgaca
     1321 gagacggaga ataccaattt acaagcatca accaggacca caggccaata acgttgcctt
     1381 atcatcaagt agcaaaggtg gagtatgtgg ctggtattct gaagcaatct cgccatctgg
     1441 atgacatcga ggcaagggag tggctgaaaa gttcgtgact tcatcgtcac atagctggta
     1501 accagtggcc tgaagagacg tttgggtgat gtacatagca tttctggata aaaatacaga
     1561 ttccctttat gggaaatgaa tctataattc ccaaagaggg aacaaaatcg gattatgaag
     1621 gtcttaaacg tagagaagct tcacagtttt agccggaagc acaatcaggc caagggggct
     1681 ttagactctt ggtatgatga agtgataaga gaaaactgga aaacgactca agacatacgg
     1741 aatagattta attctgccga cttccttcct aacaacaggg taatttttaa tataaaaggc
     1801 aataactatc ggctcgttgt ccaagttgtt taccaggcag gaatggtcat agttgaaaga
     1861 gttggaactc atgcagagta cgacaagtgg aggcttaaat gaatcgaact agctggcgca
     1921 tcattaaaaa tagtgaagag catgctgcag ctatggaaag gctcattgaa cttgcgtcta
     1981 gtgatttaca acctggaact gaagattttg atgagtttga actactaggc ttgcttatcg
     2041 agcactatga gtcacgcgag ttccctatgg acaagccaga tcccatagaa gcaatcaagt
     2101 tccgtatgga tcaacaaggc ctctcttatg ccgatatgaa acaatacatt ggctcagcat
     2161 ctaaagtatc tgaggtctta aatcgtaagc gtccattaag tctttcaatg atccgtagac
     2221 tacatgacgg acttggaatt cctgcagata tcttaattca agatatgagc gcaattgaat
     2281 ggagcctagt tgacgcagag gaagaagaaa cagccatgac tagcgtcatt gctcggtgtg
     2341 agtcagccgt cacatcacct tctgcttatt tcgctgaaaa ggctacagaa tcttactttt
     2401 caaaaatgtt gttcagcgca gtaaggggta atggcaaatg caaagaaaaa cggaatgttt
     2461 tttctttgat aagtaacttg tcatcaagtt tcacagcggc tagcaacctg aatgacgaaa
     2521 tgacttctga cggaaattac ttattattac catgaaaatt gaactcatta gcaaaaaagt
     2581 tgaacgcttg gttatgacac ggctagaagg cgattcaaca gcaaaaaaag ccataaaaac
     2641 aaccgttaat ctaaataatg aactttacac taatgtgaag gattcaaagc tatttagagt
     2701 gagatatttt gcctccgtaa ctattgaagg caggcttgag atggacatca catacgactt
     2761 tgacttccgg tcagaagatg atttttctca tgaaatggca aaatcatatg aggtaaggtc
     2821 cattgctccc aatatggcat atccctatat aaaaacatat gccgagcaaa ttatcctcat
     2881 gtcaaacctt ggtaggttca ctctgcctta ttttgatttc ttggccaatc ccatggaaac
     2941 gaacagtaat aagtgaaatt ccccacccgg cctcagcgcc gggttttctt tgcctcacga
     3001 tccccacacc taaaaacaca taaccaattg tatttgttga aaaataaata gatacaactt
     3061 gctaaacaac gcaatccaga tctccctcaa atctctttat ttatcctgtc gaattcctac
     3121 aacaaaataa aacaccataa gaatcaatac gatatttgaa aaccaagaga atttatcatt
     3181 ttgctattgc cattaattta tcattccgat aaagttcacc catcagcagg acgcactact
     3241 caccagggcg gtgaatatac aacgattcga atatgaatct acggcgctga caaagcgcaa
     3301 taaccaaagt gaactttggg gtgaatgcag aagctaacct tctcggcgga ggcgctttgc
     3361 aatgattacg cgaccggagt tagtcgcccg gctgtattca ccgccaaagt tcatcaggag
     3421 gttccatatg acacgcagaa ctcagttcaa aggcaattct cgttctcgtc gtcgtgagcg
     3481 tttaaaggca aaggcattag ctaacggcgt actggcccgc gaagaagcaa taagttcaga
     3541 agtattacac cgcccaactc taagtagagc gcagattcag gctaaaggta ctcacgaaac
     3601 gcctgatcgc atagaagacg ctaagccaat taagttcatg gcacaggacg tgatctggca
     3661 gcaggaagaa tacagacgca atctggagcg agcggccatt gtgtacgcga atgagtttgg
     3721 acataagcaa ccagaaactg gtgtatgtct tccaaatgta gctctttacg cggcaggcta
     3781 ccggaaatcc aaacaactga cagcaagatg acttgtgttg gtcgccagaa aatgaaatta
     3841 g
//
LOCUS       DAZFKM010000254         3820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFKM010000254 DAZFKM010000000
VERSION     DAZFKM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            136..498
                     /locus_tag="ACHKAY_005115"
     CDS             136..498
                     /locus_tag="ACHKAY_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703601.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            514..642
                     /locus_tag="ACHKAY_005116"
     CDS             514..642
                     /locus_tag="ACHKAY_005116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703602.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
     gene            908..1127
                     /locus_tag="ACHKAY_005117"
                     /pseudo
     CDS             908..1127
                     /locus_tag="ACHKAY_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1105..1377
                     /locus_tag="ACHKAY_005118"
     CDS             1105..1377
                     /locus_tag="ACHKAY_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO5703603.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(1401..1940)
                     /locus_tag="ACHKAY_005119"
     CDS             complement(1401..1940)
                     /locus_tag="ACHKAY_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO5703604.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            2196..3164
                     /locus_tag="ACHKAY_005120"
                     /pseudo
     CDS             2196..3164
                     /locus_tag="ACHKAY_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            3189..3320
                     /locus_tag="ACHKAY_005121"
     CDS             3189..3320
                     /locus_tag="ACHKAY_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5703605.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            3305..3457
                     /gene="kil"
                     /locus_tag="ACHKAY_005122"
     CDS             3305..3457
                     /gene="kil"
                     /locus_tag="ACHKAY_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5703606.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            3454..3612
                     /locus_tag="ACHKAY_005123"
     CDS             3454..3612
                     /locus_tag="ACHKAY_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703607.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            3714..>3820
                     /locus_tag="ACHKAY_005124"
     CDS             3714..>3820
                     /locus_tag="ACHKAY_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO5703608.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCE"
BASE COUNT         1268 a          813 c          853 g          886 t
ORIGIN      
        1 tagttaaaaa tgcacgatgt agagatattt gtgcttaaca tcaattaatc attgctaaat
       61 cgtgcttatt gttcagaaat cgcccaagtc atattgaaaa tctgttggcg aacacctata
      121 taaggagtat agttagtgac ccaaaacgta ttgccaatca acaagagctt gcatgaccga
      181 gctgttgatg aatttaatcg tctgcatgga acaatgattg gcgagattag tgcaatgctg
      241 aaaacagcta aagtagcgcc attggtggat cttcgtaaga aagatcctac cttttcgaac
      301 gttgtcgcag aattacggac attcagagac gtatgtaatg ctctgctccc atactttcgt
      361 gtagataaga ccagcgaaat cgctgtcatc gataagttac ttatactggc taatgacctg
      421 gctcaagcca tcgatgctga cgatcctgac gcattgtgcg cagcaatagc agcccttgat
      481 gttgagcctt acatttaaac gaggggaaaa aaaatgacta aagcgtttga ctatactgcc
      541 gttagtaagc ttttggctga aatgcgcgga tgtgtagagc gcgttcaaaa tctacgccgc
      601 gactttgaag ctcacatcaa tcattcacaa aaagcggctt gatacttaag ttagatgaca
      661 gaacccggcc tcagcgccgg gttttctttg cctcacgatc cccacaccta ataacacctt
      721 aaccaattgt atttattgaa aaattgatag atacaacttt ctaaaccacg aaattctgat
      781 ccccctcaag tctcttcatt ccttctgata aattcccgca atcaaataaa aaaccataat
      841 aatcaacagg atatttgaaa acccaaacaa tttatcattt tgctattgcc atcagtttat
      901 catttcgata aagtcaactc atcagcagga cgctgtaagc caaacggagc agactggcag
      961 gctctttaaa caacgtcgaa cactcgacta cgtggctgaa aagccagatc acccaaccac
     1021 ataagctgtg ggatgcaatg ccgaagtaac cgtctcagga ggagcttcga gattgcatcg
     1081 ccaaagttta ttcgggagga atctatgtcc agaaaaacag aatttaaagg caccgcagct
     1141 tctcgccgta gagctcgtcg cgcaaacctg caaagtcagg aggcgattag ctccgacaag
     1201 ctacacaggc caaccccttc acgagtggtc ttgcaatgca agcgcaaacc agcaatgaga
     1261 gcagaagtta taactctgac aacgttgacc agaaaatatg aaggttcaac ttgtcttcca
     1321 aacgtagcca tttacgcggc aggctaccgg aaatcaaaac aactgacagc gaggtgacga
     1381 tcccccccgc cgtagaggaa ctaaattagc tcatcagctt taccagcaaa gcgcatataa
     1441 cactcatgga accagacgtc aggtataaca aatttctctt tacctgacgc gctgtaagtc
     1501 acacctgagc gatggagaag gccttttctc ataagagata aagcgaccgg atcgccgtgc
     1561 ttaagtacga ttttgttatt tgccataaca gcaaatgcta aaacctcttt ttcctgaata
     1621 ctcagagaat tgaataggcg ctcagtctcc acaattactt tagcctcatc tctcttactg
     1681 agatattttt tatatctgtc tgaagatgcc agaaatataa actccatccc cctgttcaga
     1741 acataactga tacacaaaag cattgcgtaa tacatccagt gatctggaag tatttcgggg
     1801 ttccttatat ctacccactc tttaattgat gctggggtga ttattatcaa ggcaattaaa
     1861 ataataagca tatgaattaa ctgttgtagc gtaagaccac gcaggaagaa acgtaatagt
     1921 tcctgccacc agttactcat cggtgaatct ccatcattct ctctgtaggg gtgaatagag
     1981 tttatccgat ttctcgctgt aggggtacac gagaaccacc gagcctgatg tggttaaaag
     2041 acaggcatac gaataaacac tgcactgtgt attcattcca acgagtgaat acacggagca
     2101 atgtcgctcg taactaaaca ggagccgact tgttctgatt attggaaatc ttctttgccc
     2161 tccagtgtga gggcaatttt tttgacggag gatatatgag tgaagtaaca gatttagttg
     2221 ttattgaaaa agcaaatgca atgactgtaa tttagtctgc cgaccagatt gaagaaattc
     2281 ttcaaaaggt tgaacgtgaa gttatgtcct ttgtgcctga tatcacaacg gcaaagggca
     2341 gaaaggagat cgcttatctg gcgtataaag ttgcgcagac gaaaacatat ctcgatggtc
     2401 ttggcaaaga ccttgttgct gaactgaagg aaattccaaa gctaattgat gctaaccgca
     2461 agacagtgcg cgatcgcctt gatgaactga aagccaaggc gcgccagcct cttactgatt
     2521 atgaggaaga acaggcgcgg attaaagccg aagaagaagc taaggcagca gctgaagctc
     2581 tcgcaaagca aattgagtct gaccatgaaa tagcgatttt gatggatcgc gaatttgacc
     2641 gccaaagaga agaggcaaga ctcaaagcgg agcaggaaaa gcgagagcat gaagaacgct
     2701 taaaaagaga agctgaagag aaagccagag ctgaagccga agcaaaggca aaagccgaaa
     2761 ttgaagcagc agcaaggcga gaagcagaag ctaaggccgc agaggaacgt gcagagcgtg
     2821 aacgcattga agccgagcaa cgagcacagc gcgaagcaaa agaggcagca gaacgagctg
     2881 aaagagaaaa gcatgcggca attgaagcag aacgccgtaa agcacaggag gaggcagaac
     2941 gaatcctgcg cgaggctgaa gcaaaagagc aagccagaat agcagaagaa aaaagaatca
     3001 aggacgaaga agagcgtaga gcaaaggata aagctcaccg gaaagaagta aataacaaaa
     3061 tacttgctga ccttatcaag gttggcgcat cagaagatgt tgctaaaaat atcataacag
     3121 ccatcgtaaa aggcgaatta ttcgcaacaa aaataaccta ctaataaaac caacataagg
     3181 aaccacccat gatttacgca atcgcgggag gcgctcgcat gggtgccttc caattaaatg
     3241 aatctttact tgaacgaatc acccgtaaat tacgtgacgg atggaaaaga gttgaggtct
     3301 tattatgcgc aatgaaatag ccatcaatca ccagatgctt cgtgctgcac agaacaaggc
     3361 agtaatagcc cgatttattg gtgattcaaa aatgtggctt gaagcaaata aagcgatgaa
     3421 atcagctatc aaccttccgt gttatcgcag gaaatgagtt ttacagataa ctggtcagac
     3481 gaagaattca ttcgtcagat gaaagaatta atcggtaacg aaggagatat tcatgtcact
     3541 tgcaaccaca gtgaaggaga gcaagttaca gaggcgcatg tacacgcaga aagctctctg
     3601 gtatcgccat aatggtgacc gcgaaggaat gcgggtatgc cttaatttgt cccgagtcga
     3661 agtattaaat cagcgttatt tccttgggcc gtgtccattc tgagaacaat catatgagca
     3721 aagaatttta cgcaagactg gcagctattc aggagaatct gaacgcgcca aagaatcagt
     3781 acaactcatt cggcaaatat aaatacagaa gctgcgaaga
//
LOCUS       DAZFKM010000255         3815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFKM010000255 DAZFKM010000000
VERSION     DAZFKM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /locus_tag="ACHKAY_005125"
     CDS             <1..450
                     /locus_tag="ACHKAY_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5703609.1"
                     /translation="DIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHRE
                     DDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHH
                     LPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            447..1028
                     /locus_tag="ACHKAY_005126"
     CDS             447..1028
                     /locus_tag="ACHKAY_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5703610.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1371..>3815
                     /locus_tag="ACHKAY_005127"
     CDS             1371..>3815
                     /locus_tag="ACHKAY_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5703611.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRSFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTV
                     QFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVGKSAF
                     VEAVGRASDDAEGYLDF"
BASE COUNT          845 a         1013 c         1191 g          766 t
ORIGIN      
        1 gacattcaca aattccgctg tgtgccacat ctgacaggac ggcgctttga gcacggggtg
       61 acggactgtt acacgctgtt ccgggatgct tatcatctgg cggggactga aatgccggat
      121 tttcatcgcg aggatgactg gtggcgcaac ggtcagaacc tttacctgga caatatggcg
      181 gtcaccggct tttaccgggt gcccctgtcc tctgcacagg cgggcgatat tctgctgtgc
      241 tgctttggtg cttcggtacc gaaccatgcc gccatttact gcggcaacgg tgagctgctt
      301 caccatctgc ctgaacaact gagtaaacgg gagaggtatt ccgaaaaatg gcaacgacga
      361 acgcattctg tctggcgtca ccgccactgg cacgcatctg ccttcacggg gatttacaac
      421 gatttggccg ccgcctcagc ctgtatgtga acacggcagc ggaagccatc cgggcgctgt
      481 cgttacaggt gccgggcttt cgccgtcaga tgaacgaagg ctggtaccag atacgtattg
      541 ccggtgatga cacggcaccg gaggcggtgt acgcccgtct tcacgaacag ctgggtgagg
      601 gaacggtcat ccatattgtg ccgcgactgg ccggggccgg aaagggtgga ctgcagattg
      661 tgctgggggc ggcagccatc gtgggctctt tcttcactgc cggggcatca atggcgttat
      721 ggggttcagc cctggcagcc ggtggttttt ctgccaccac aatgctgttt tcacttggag
      781 ccagcatgat tctgggcggt gtggcccaga tgctggcccc gaaggcaaaa acaccggatt
      841 accgcgcaac ggataacggc agacagaaca cgtacttttc ctcgctggat aacatgattg
      901 cccaggggaa cccgatgccg gtgccttacg gggaaatgct ggttggctcc cgccgtatat
      961 cccaggacat cagcacccgt gatgaaggcg ggggcggaac ggtcgtggtt gtcgggcgac
     1021 agggataaaa cataaaaaaa tcccgcagtg atcgcggagc tgcggggaca gacaaatgaa
     1081 gatcaatgtt aaggagttgt ttttgttact cgggcaaaaa aacactaacg cagcgaaatt
     1141 ataagcgcca cagtcagtgt gtgaaaatgt gaagatattc agaattttta tgccattacc
     1201 ggttttaacc aacaggatta tcggtgggca tgaaagaaaa ccccggtatc tgctgatacc
     1261 ggggtttctc tttagcatgg cagaaatgtg tttcatgctt ttcgggcgaa ggatatccga
     1321 cttctgtacg gaatggcaag tggcggttaa tttattcagg ggaaggctgt atgggaaaag
     1381 gtggcggtaa ggcacacacg cctcgtgagg cgaaggataa tctcaaatcc acgcagatga
     1441 tgagtgtgat tgatgcgatt ggtgagggac cgatagaagg tccggtgaag ggactgcaga
     1501 gtattctggt gaacaaaacc ccactgacgg acacggacgg caatcccgtg atacacggtg
     1561 tgaccgcggt ctggcgtgcc ggggagcagg agcagacacc accggaaggc tttgagtcct
     1621 ccggagctga aaccggactg ggcgtggaag tgacgaaggc aaaaccggtg acgcgcacca
     1681 ttacgtccgc gaacattgac cgcctgcggg ttaccttcgg ggtgcagtca ctggtggaga
     1741 ccacctcaaa gggtgaccgt aacccggcat ccgtccgcct gctgattcag ttacagcgta
     1801 acggtaactg ggtgacagaa aaggacgtca ccattaacgg caagaccacc tcacagttcc
     1861 tggcctcggt gattctggat aatctgcctc cccggtcctt taacatccgg atggtcaggg
     1921 agacggcgga cagcaccacg gaccagctgc agaataagac gctgtggtcg tcatacaccg
     1981 aaatcatcga tgtgaaacag tgctacccga acacggccat tgtggggctg caggtggatg
     2041 cggagcagtt cggcggccag cagatgacgg tgaactacca tatccgcggt cgcatcatcc
     2101 aggtgccgtc aaactatgac ccggaaaaac gcacgtacag tggtatctgg gacggcagtc
     2161 tgaaaccggc atacagcaac aacccggcct ggtgtctgtg ggacatgctg actcacccgc
     2221 gctacggcat gggaaaacgt ctgggggcgg cggatgtgga caagtgggcg ctgtatgcca
     2281 tcgggcagta ctgcgaccag acggtcccgg atggtttcgg ggggaccgag ccgcggatga
     2341 cctttaatgc gtacctggca caacagcgta aggcgtggga cgttctcagt gatttctgct
     2401 ctgcgatgcg ctgtatgccg gtatggaacg gtcagacgct gacgttcgtt caggaccgcc
     2461 cgtcggatgt ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat aacggcgtgg
     2521 gattccgcta cagcttcagt gccctgaagg accggcacac ggcggtggag gtgaattaca
     2581 ccgacccgca gaacggctgg cagacctcca cggaactggt ggaagacccg gaagccatac
     2641 tgcgctacgg acgcaacctg ctgaagatgg acgcgttcgg ctgtaccagc cgcggtcagg
     2701 cccaccgtgc cggactgtgg gtgataaaga ccgaactgct ggaaacgcag acggtggatt
     2761 tcacgctcgg gtctcagggg ctgcggcaca cacccggtga cattattgaa atctgtgata
     2821 atgactatgc cgggaccctg accggcggac gtgtcctgtc cattgatgct gccacccgca
     2881 ccctgacgct ggaccgtgag gttaccctgc cggagacagg tacatcggcg gtgaacctga
     2941 ttaacggcag cggtaagccg gtgagtgtgg acatcaccgc acaccccgcg ccggaccgga
     3001 tacaggtcag taccctgcct gatggtgtgg agacatacgg ggtgtgggga ctctccctgc
     3061 cgtcactgcg ccgtcgcctg ttccgctgtg tctccgtccg ggaaaacacg gacggcacct
     3121 ttgccatcac ggcggtgcag cacgtaccgg aaaaagaagc catcgtggat aacggtgccc
     3181 gctttgagcc gcagtcaggt tccctgaaca gcgtcatccc accggcagta cagcacctga
     3241 cggtggaggt gagtgcagct gacggccagt atctggcgca ggctaaatgg gacacgccgc
     3301 gggtggtgaa gggcgtgcgc ttcagtctgc gcctgaccag tggtaaggga acggatgcca
     3361 gactggtgac caccgccatc accgcagaca cggagcaccg tttcagcggc ctgccgctcg
     3421 gggaatacac cctgacggtg cgggcgataa acagctatgg ccagcagggt gaacctgcca
     3481 ccaccacctt ccggattgcc gcaccggcag caccgtcgcg gattgagctg acgccgggct
     3541 attttcagat aaccgccacg ccgcatcttg ccgtttatga cccgacggta cagtttgagt
     3601 tctggttctc ggaaaagcgg attgcggata tcaggcaggt tgaaaccgca gcccgctatc
     3661 ttggctcggc gctgtactgg atagctgcca gtatcaatat caaaccgggc catgattatt
     3721 atttttatat ccgcagtgtg aatactgttg gcaaatcggc attcgtggag gctgtcggtc
     3781 gggcgagcga tgatgcggaa ggttacctgg atttt
//
LOCUS       DAZFKM010000256         3777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFKM010000256 DAZFKM010000000
VERSION     DAZFKM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2972)
                     /locus_tag="ACHKAY_005128"
     CDS             complement(<1..2972)
                     /locus_tag="ACHKAY_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFO5703612.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMTYFSEQDLAIINAHRK
                     PASRFGFAVLLCYLKSIGFAPDKKSSPPAKLLKTIGARLKLSAELWQEYVSGRDTTRR
                     EHMRELYRYLELKAFNGALQKECIRHLLPLSTRTDRGILIAEELLKYLHQQRIIIPGI
                     DVLERTCAEAMTLGDKTVYTTLNAHLDITHKSALDALLVTNEKQISRLTWLLQPPGKI
                     NGKNVLQHIDRLNAIEALALPEGVERTVHQNRLLKLAREGRKMSSRDLADFSPARRYA
                     TMVCVLTEARATIIDEIIELHERILGSMFSRAKHQQAERLQKTGKLIQQKLRQYVSVG
                     QAILDARNAGEDPLSAIERVLPWDDFVASLEETKLLARKDNFEPLHLITEKFSTLRKY
                     SFRFLSALQLRATSAALPLNDALETIRDMYRKQLRKVPASAPLDFIPESWRKMVVTPS
                     GIDRQYYEICAMNELKGALRAGDIWVRGSRRYKNFDDYLMPNDDFEKLLNNNLLNLPV
                     GTECGAYLNARLTLLASRLEEVNAMAVAGELPDVDISDKGVKITPLDNSVPSTVSPLA
                     DLIYNMLPHPKITEILDEVERWTAFSRHFTHLKNPLIRPQDNRLLLTTILADGINLGL
                     AKMAESCPGTTRASLENIQAWYIRDETYSAALAELVNAQKSRPLAALWGDGSTSSSDG
                     QNFRVGSHGRYAGQVNLKYGQEPGVQFYTHISDQYSPFYTKVISRVRDSTHVLDGLLY
                     HESDLDITEHYTDTAGFTEHVFALMHLLGFAFAPRIRDLHDKRLFIQGKADNYPALQS
                     IISTTPLNIKEIKIHWNEILRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGR
                     IERTLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFMHRLGEIRDRGIENQSYRAS
                     GLTLLTAAITLWNTVYIENAINLLKKKGLHINEQLLSHLSPLGWEHINLTGDYIWKNN
                     KKLAEGKYRPLRAVDVSQ"
     gene            3139..>3777
                     /locus_tag="ACHKAY_005129"
     CDS             3139..>3777
                     /locus_tag="ACHKAY_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO5703613.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLDRPELKRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYKEVKPD"
BASE COUNT         1030 a          876 c          869 g         1002 t
ORIGIN      
        1 tactgactga catcaactgc tcgcaaagga cgatacttcc cttcagccag cttcttattg
       61 tttttccaga tatagtcccc tgtcagatta atgtgctccc agcccagcgg agacaaatgt
      121 gacaacaatt gctcattaat atgtagccct ttctttttca gcaggttaat ggcgttttct
      181 atatatacgg tattccacaa agtgattgcg gcagtgagca gcgtaagccc actggcgcgg
      241 taactctggt tttcaatacc acgatccctt atttctccca gacgatgcat gaagacagct
      301 ctggcaaggg cattccgggc ttcccctttg ttcagacctg cctgaaccct acgacgaagc
      361 gccggatcgc ggaaccagtc cagcatgaac agtgttcgct caatacgacc aatttctctt
      421 aacgccttag caaggccatt ctgtttcgga taactggcca attttttcag catcagagaa
      481 gcggtcactg ttccctgttt tattgaggtc gccagtctca gaatttcatt ccagtgtatt
      541 tttatctctt tgatattcag gggggttgtg gagataattg actgtagagc aggatagtta
      601 tcagctttac cttgaataaa caaacgtttg tcatgcagat cccggatcct tggggcaaaa
      661 gcgaatccca gcagatgcat tagtgcaaaa acatgctccg tgaaacctgc tgtatcggtg
      721 taatgttccg ttatatcaag gtcactttcg tgatacagaa gaccatcaag tacgtgggtg
      781 gaatcacgaa cacgactgat tactttggta taaaacggac tatattgatc tgagatatgc
      841 gtataaaact gcaccccagg ttcctgaccg tacttcaaat taacctgacc ggcatagcgc
      901 ccatgactgc caaccctaaa attctggccg tcagaggatg aggtggaacc gtctccccat
      961 aatgccgcca gcggtcgtga cttctgggca ttaaccagtt ctgccagtgc agcagagtac
     1021 gtttcatcac ggatgtacca tgcttgtatg ttttccagcg aagctcgtgt ggttcccgga
     1081 caggactctg ccatttttgc caaccctaga ttgatgccgt ccgccaggat agtggttagt
     1141 agtaaacgat tatcctgagg acggattagc ggattcttaa gatgagtaaa atgtcgcgag
     1201 aaggcagtcc atcgctcgac ttcatcaagt atctctgtaa ttttggggtg cggtagcatg
     1261 ttgtaaatca ggtcggccag gggagagact gttgaaggaa cgctgttatc cagtggggtg
     1321 atctttacac ctttatctga aatatccaca tcaggaagct cccctgcgac agccattgcg
     1381 ttaacttcct ccagacggga ggccagtaga gtcagtctgg cattaagata agccccgcat
     1441 tctgtgccaa caggtaaatt taataggtta ttatttaata acttttcgaa atcatcgtta
     1501 ggcataagat aatcatcaaa gtttttgtag cgccgggagc ccctgaccca gatatcaccc
     1561 gcccgcagcg ccccctttag ctcattcatt gcgcaaattt catagtattg tcggtcaatg
     1621 cctgaggggg tgaccaccat ctttctccag ctctcaggaa tgaaatccag tggtgccgat
     1681 gcaggaacct ttcgtagctg tttacggtac atatcacgaa tagtttcaag ggcatcatta
     1741 agcggcaacg ctgccgaagt ggctctcagt tgcagggccg atagaaaacg aaaagaatat
     1801 ttgcgcaatg tactgaattt ttcggtaata agatgaagcg gctcaaaatt atccttacgt
     1861 gcaagaagct ttgtttcctc cagactggcg acaaaatcgt cccacggtaa cactcgttca
     1921 atagcagaca gaggatcttc gcctgcattg cgtgcatcca ggatagcctg gccaacagaa
     1981 acatactgac gtaacttttg ctgtatcaac ttgcccgttt tctgaagacg ctcggcctgc
     2041 tgatgttttg cccgactgaa catgctgcct agaatacgtt catgcagttc tataatttca
     2101 tcaatgattg tggctctggc ctcagtcagt acacagacca tcgttgcgta tcgacgagca
     2161 ggggaaaaat cagccagatc gcgactgctc attttgcggc cctcacgcgc gagtttcagc
     2221 aatcggttct ggtgaacggt tctctccact ccttccggca gcgccagagc ttcaatagcg
     2281 ttaaggcggt caatgtgttg caggacattc tttccattga ttttccctgg aggttgaagc
     2341 agccacgtca aacgggatat ttgtttttca tttgtcacca acagggcatc aagcgcagat
     2401 ttatgcgtga tatcaaggtg cgcattcagc gttgtataca ccgttttatc acccagtgtc
     2461 attgcttctg cacaggtacg ttccagaaca tcaatgcctg gaataataat tcgctgttga
     2521 tgcaggtact ttagtaactc ctctgcgata agtatgcctc tgtccgttct ggtagacaga
     2581 gggagcagat gccggataca ttctttctga agtgcaccgt taaaggcttt cagttcaaga
     2641 tatcgataaa gttccctcat atgctcacgt ctggtggtat cacgaccgga aacgtattcc
     2701 tgccaaagtt ctgctgataa cttaagtcga gcgccaatgg tttttagcaa ttttgcgggt
     2761 ggggatgatt ttttatccgg ggcaaaccca atacttttca gataacagag aagaacggca
     2821 aaaccaaacc ggctggcagg tttacggtga gcattgataa ttgccagatc ctgctcactg
     2881 aaatacgtca ttcgagtcag tgttaactca tcgtctggca atgccagtaa tgattctctt
     2941 tccgatagag aaagaatctg cctccttgcc atatagttat ccttgttaat tatttagtac
     3001 gttatttttg atgaaaaggt tattgcataa gggtacaccc tgatgcgaga tataaaatgt
     3061 gacctcatac aaataatgac ataacctata tgcgatagaa tatatgcaac aacctaaatg
     3121 cgacaatgcg gtgaaaaaat gttcatcaga gcttatttaa gggcgtcaac agaagatcag
     3181 tttgcagatc gagcaaaaga gatgctggaa cagttcgtcc aagaacgggg gcataaaatt
     3241 gccagttatt atcgggagaa cataagcggt acaaaacttg accgtccgga actgaaacgc
     3301 ttactgatgg acagtcaccg taatgatatt ttgctggtcg agcagataga ccgcctgacc
     3361 cgactgagta acagcgactg gataacgctg aaaaagcaga tagaacaaca cgaactgcgg
     3421 attgtcagtc tggatgttcc aacatcatgg caagccctgt cagataaaga tccttcacaa
     3481 tcagatccga ttacccgcgc agtgataact gcaataaata atatgcttat agacctgatg
     3541 gccgcgatgt cgcataagga ctggctgagt cgtcgccaac gccaaaaaca gggaattgaa
     3601 cgagctcata cgttgggtaa ataccgggga aaacaggccg atcaggaacg tcatcagaaa
     3661 gtgttgtact acagacaagt aaaaaaactc agcattagag aaacagcaga tgcaacgggc
     3721 tacagcactt cgcaggtttg tcgcattcag gctctctata aagaggttaa acctgat
//
LOCUS       DAZFKM010000257         3770 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFKM010000257 DAZFKM010000000
VERSION     DAZFKM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(85..1623)
                     /gene="cadC"
                     /locus_tag="ACHKAY_005130"
     CDS             complement(85..1623)
                     /gene="cadC"
                     /locus_tag="ACHKAY_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO5703614.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1883..1997)
                     /locus_tag="ACHKAY_005131"
                     /pseudo
     CDS             complement(1883..1997)
                     /locus_tag="ACHKAY_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2372..2521)
                     /locus_tag="ACHKAY_005132"
     CDS             complement(2372..2521)
                     /locus_tag="ACHKAY_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO5703615.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
     gene            complement(2521..3011)
                     /locus_tag="ACHKAY_005133"
                     /pseudo
     CDS             complement(2521..3011)
                     /locus_tag="ACHKAY_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            complement(3103..3678)
                     /locus_tag="ACHKAY_005134"
                     /pseudo
     CDS             complement(3103..3678)
                     /locus_tag="ACHKAY_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT         1038 a          792 c          803 g         1137 t
ORIGIN      
        1 aattgccgca acatattata ccaacagaaa catacaaaaa ctcaacaaca aatattcccg
       61 agcataaatc aacccgggag ttacttattc tgaagcgaga aatttgtcga gataaggtac
      121 aacataagga acagaagtct ggaatatacc attttcaatc cagtaaaggg tgtttgcccc
      181 tgggcgtaaa ttaaaggcgg tgagatatgc atcagccgct tcccggttca tccccttcat
      241 ttcataaacc ttgccaagca acacataatt tagccaggac atttcaagat caatgccagt
      301 atttatcgcc tggtaagact catctgtttt accttttacc agagcactga ccgcttttat
      361 ttgatatata atggacaggt tattcaattc cggcagtgta acaatgttgt ctatttctgt
      421 gttcagtgct gctaattgtt tttcatctaa aggatgttga gaatggcgca cgatatcaac
      481 taatgctttt tctgctctcg cgtaggtaaa ttctggggat gattgaacaa tctcacctaa
      541 taattcactg gcacgattca atgatttatc atcgccatgc agtaaataat catgtgcctg
      601 ataaaaatta gttaataacg caccacgatg aggcaatatt tgctggagcg tctcctgcat
      661 tcgttgtggc cacggttggt ttaacgcttt tgataaactc tccagtaaat cattttgaat
      721 cgccagctga ttaccgttag tgatgacata acgtttatcc agcatggttg aaccatctgc
      781 attgtctacc aattttatcg acataaagca ttgttgagca cggtattggc gctgattaac
      841 aaacgcaata gataatgttt taccggaact gctcggttca tcaatgttgt agttgatttt
      901 gtcatgcacc ataaaggtgg agaaggtgtt aagtgatgtc gccaccaaat cacccacgcc
      961 tatcgcgtaa gagagctgat acggggaact ccagctgtta caacttttat ttaccatatt
     1021 aatgtcaata tcgcgtggat tgagcaaaat acgcgatttg ctcataggaa gacgtgtatc
     1081 aagagttgaa aacgctacca gtgctacaca gatacctaac gacaacagga aaaaaaacca
     1141 tacccaaaag gtagtgaatc gtttgctttt aactggggat tgttcaggtg gcgttgcggt
     1201 gttttgaatg ttaagactgt gggagggaga atctgtggca ggaaccgcct ctggtatagg
     1261 gggaggcgaa gatagcatta tttcctctcc ctcttcttcg ctgtaccaga taaccggcac
     1321 cattaattta tagccgcgct ttggtacagt agcgatatag acaggactat cttcatcatt
     1381 atcttttaat gacttacgta gttctgagat actctgcgtc acaacatgat tggtgacaat
     1441 acttctcttc cagacattat cgataagttc atccctgcta agtacttcgc cactgtgttg
     1501 agcaaagaaa accagaagat cgattaatct cggctcaagg gtaagttgac gcccattgcg
     1561 gctaatttgg tttatggacg gagtaacaag ccattcgcca acgcgaacta caggttgttg
     1621 cataatagaa actcattcga aaagggaatg atgcaatgat aattgccaca acctattttt
     1681 accatctata gatgggttta tttacatatt attggtgaat gcaagacgtt atttttacca
     1741 gccataaact tcttgattac atagtattac gaaaggattt tactgagaac cagaagtaat
     1801 attccttacc atcaaaattc atcatctttg ccaaagaaaa atgttcagaa aataatccat
     1861 ggaaaattgt ccggagcgct tactatttta atggattgtt agtctttgca tgagcaagcg
     1921 gactgataca tttctctttg ttctcattca gaaaatctca tcagtcggcg ttcagaccgg
     1981 tgttgtgctt tatggatgcg ttacgctcct gatgacgtca tttgacgttc aacaacatca
     2041 cggggccgca cgacatttca cgtcagttag tgctatcgct caggaacaaa ttttcccgaa
     2101 ttgggatatg cccgcaaatt gctggtgatg tggggagaat ctggttgagt tcgggagaat
     2161 tgatttggag gcagaacgct taaattgtgg cgtccagaaa cgaaaaacgg acctccgtgg
     2221 aggtccgttg atatgaattt ggtgccggac tcggaatcga aaatttcctt tatcatattg
     2281 atattaatgc aatttgtttg gtttgattaa aaatgcaccc tcaattatac ccgcagtccc
     2341 ttttgatatc gaaagggaat cacactcggg atcaggctga gagcacagaa ggaaagtacc
     2401 tggcttccct ggcctgttca atacagggca gcagtgctgc cgaatccgtt ttatcctcat
     2461 cgttgtcaaa gataattccc gattcgcagt cgatattgtc ctgcagccac gcaatgagga
     2521 tatacggaga agcacatccg tatcatcagt gtgacgactg cggcggccat ccatccagtc
     2581 atcggttcgt ctgagaatga cgtacaactg cgcacgcgac acccggagac aacggctgac
     2641 taagcttact ccccatcccc gggcaataag ggcgcgtgcg ctatccactt ttttgcccgt
     2701 ccatattcaa cggcttcttt gaggagttca ttttccatcg ttttcttgcc gagcaggcgc
     2761 tggagttctt taatctgctt catggcggca gcaagttcag aggcaggaac aacctgttct
     2821 ccggcggcga cagcagtaag acttccttcc tggtattgct tacgccagag aaataactgg
     2881 ctggctgcta caccatgttg ccgggcaacg agggagaccg tcatccccgg ttcaaagctc
     2941 tgctgaacaa ttgcgatctt ttcctgtgtg gtacgccgtc tgcgtttctc aggccctaag
     3001 acatcaatca tctgttctcc aatgactagt ctaaaaacta gtattaagac tatcacttat
     3061 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagttttttg ttatgttggc
     3121 tgagtgacag gatgttaatc tgatgtcttc tacttcatga agattatgtt ctaccagacg
     3181 attgacgata tcagttggtg ataaaaattc atctccacac ttaagaagag gtaggccagc
     3241 agaaccatcc cctggcagat aaattttatt gtatttttct ggtgttaaat cctttggtag
     3301 gatagttttc atctctgttg ccgcaatact tatagctgct gcggcagcaa catcggtgtt
     3361 acttgattgt agataacttc tgtttgcacc aggaaaggta aactctgttt tctttttatc
     3421 aaaatcttgt tttgtagctt cgtgcaggaa taatatatca taaggttttt tccctgttgt
     3481 gactcttgaa acattttgac caagaatatt aagagcatat tcacctacgg attttgttcc
     3541 taaaaacaca agcaatcctt ctttctctgg cactgtctct atttttctcc ctccaggaat
     3601 tgtggagatg gattgagatt gttctgccgg aattgatacg ctggcgctta taggtagctg
     3661 agatcttata ctaaacatac aacctccttc ttagtttctt taaatgtgag ttcatgataa
     3721 ttctaatgga gtgagtaata ttttagttga taatagctga tatccttttc
//
LOCUS       DAZFKM010000258         3731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFKM010000258 DAZFKM010000000
VERSION     DAZFKM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..277
                     /locus_tag="ACHKAY_005135"
     CDS             92..277
                     /locus_tag="ACHKAY_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5703616.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(407..547)
                     /locus_tag="ACHKAY_005136"
     CDS             complement(407..547)
                     /locus_tag="ACHKAY_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703617.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(904..1128)
                     /locus_tag="ACHKAY_005137"
     CDS             complement(904..1128)
                     /locus_tag="ACHKAY_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703618.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1193..1399)
                     /locus_tag="ACHKAY_005138"
     CDS             complement(1193..1399)
                     /locus_tag="ACHKAY_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5703619.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            complement(1627..1773)
                     /locus_tag="ACHKAY_005139"
     CDS             complement(1627..1773)
                     /locus_tag="ACHKAY_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703620.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            complement(1773..2339)
                     /locus_tag="ACHKAY_005140"
     CDS             complement(1773..2339)
                     /locus_tag="ACHKAY_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5703621.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMIDFVLADTGKGKITKAD"
     gene            complement(2610..3143)
                     /locus_tag="ACHKAY_005141"
     CDS             complement(2610..3143)
                     /locus_tag="ACHKAY_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5703622.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(3194..3538)
                     /locus_tag="ACHKAY_005142"
     CDS             complement(3194..3538)
                     /locus_tag="ACHKAY_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO5703623.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            complement(3543..>3731)
                     /locus_tag="ACHKAY_005143"
     CDS             complement(3543..>3731)
                     /locus_tag="ACHKAY_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5703624.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKTARGD"
BASE COUNT          983 a          998 c          791 g          959 t
ORIGIN      
        1 cgggtattca gtgttttgat tctgctctgg agtgtacgaa gctcagccat gtaaaatccc
       61 cgtcatatgg caatcagtaa aggaaataaa tatgtcatcg aaaaaccgga cccgcagaac
      121 cacaacccgc aatatccgtt tccccaatca catgattgaa cagatcaata tcgcccttga
      181 acataaaggt tccggtaact tttcagcgtg ggttattgaa gcctgcagaa gaaggctgtc
      241 aacagagcgt tcgggtatga attacataat taagtaacat ggtgttcaca gaacacacag
      301 ttaccggaca catcagtttt ccattcgtcc cccggcagta caggcttccc gtctgacggg
      361 atagcctgaa aaaacacaga aaattatttg ttataattaa tataacttac tcaaaaaaaa
      421 gcgacgagaa aatcagcatc aacgagcaat aagcgccaat acgtgataac aaatggcagc
      481 catatttatc tgcagtataa gcaatggaca ggataaccac accagaaacc gtcagcataa
      541 aatccatttg aacttccccg gacaaaatcg actcatctaa agatttacag ctctttttat
      601 tatcaatatg ttaaaagtaa aataaacaga tgttcaataa tacgaataca aaaacgtgct
      661 gaaaatcaat gaatccattt ctgtgttatc aattaatagt gataaacatc cggcttcttc
      721 caccatcgca ccggaccagc gaccatgagg ggacaacgcc gcgctccgtt aacgcggtaa
      781 accccggtgt gtatcgtttt tgattatccc cgcacactcg cgcagaggat tctcccggtc
      841 gggctgcggt ctctgttaat gcaggaatac ggcgacaata ccgcgcatga ataataaggt
      901 cgctcaacac actggctgta atgcagcgga taccatgcgg catttagcgg cattcatcgt
      961 acactcaacg gttagctctt cattcgtggc attcacctga aaggtcctgg agtgtaattg
     1021 cgtacattta ccactgaacg aaccttcaac aagaacacga ccacgctgca aaatacggaa
     1081 cggaattgtt ccctgaaaag gctttacggt taccaataat ttcttcatgc attctccgaa
     1141 taacaaaaat actagttaat acactgagtg cggatatatt cctgaagcat tctcaatgct
     1201 gcctggtcgc tgatgattcc gtctctgata ccgagaacgt ttcgtccagc aaccggagag
     1261 agttcgacgg cggcatcatt gcccacgccg gaggtgccgg tggcttcacg cacggtaccg
     1321 gggcaggtgg cgttgatccg caggcgctta cgaccagcgg caacatcagc acgcagagtt
     1381 tcattttcag ctctcgcatc ggctaattcc ctcgagtatt ttgcatcgag cgcagcaaca
     1441 tcacgctggc gcacctgcat gtcagtaatg gtggcattcg cctgttccag ctctctggct
     1501 tttttatcgc gctgcgcttt gtaggtgagc gcgttgtcac ggtaatggtt tgttgccagc
     1561 cacagcgcac cacagccaac cgccatgaca ataatcacca cacacagaac acggttcatc
     1621 tctctttcac cccaccagtc ccgataacgt caggactcgc caggcggtgg aaaagaaaat
     1681 ggcaaccagc atgactaaaa atgaaatgcc gacaagtaca cagaggctct tcaccagcgt
     1741 tatgagttta tctgatatca ttagccaccc catcaatccg cctttgttat tttccctttg
     1801 cctgtatcag ccaggacaaa atcaatcata ttcgcttcat ttaccagcgt acggattttt
     1861 gatacatgcg cggctttaac ctgtttccac tcattcagcc cggtagcaaa cacactggca
     1921 atgtttttat cccgtttcat gtcagcacaa gcctggttga gttcttccat cacgctcatt
     1981 ttacggggat taacgacaaa acccttcgtc cagtactcgt aaagaacatc gtcgcactct
     2041 tcctgatacc ggatgacctt atcgcggatt tcgggtttta ctttgttggg attaatggtt
     2101 tgtagccagc cggcaagttt tcgaagtggc atggacacca tattgcgttg tttcccatcc
     2161 tcagcaacca taacgatttc cgttatagtt gacgcaaaac gctgtcttaa cttagccaac
     2221 tgtgattgcc aggccagccc catccccgca acgacaggtt tcatgggaac gtatggttcg
     2281 ccattatggt taactacata aagagagttg ccgtgaaacg gcacggccat catattcatc
     2341 ggttatttcc ttttagtgat gaaccttgtc tcacaggaat ccagcccaca gaaaggcacc
     2401 gacagccaaa ccggtatcct caagggtcat cctgaaaggt tctgtgttgt gagatgcgcg
     2461 tgagatgcgc agaaatgaca aaggcatcat tacggtgcct gagtgttaaa caactgtttt
     2521 gactttattc acttacattt tgccaatttg caggatttcg tgttatccat ccatgtaagc
     2581 aaacctcatt tttcagcaaa atattctgct tatctgtcga ttccccagca cgccagcgcg
     2641 ctctcctggt cacgacggga tacctgaccg taacagttat ttgagcgaat acggcagtcc
     2701 ctgccaccgt ccttaatcca ccagcgaata gcttcgcagg caccttttcg atctcctgca
     2761 ttaatccgtc tgtaaaacgt cgacgggaaa cacttaccgg ggccaatgtt gtacggacag
     2821 aatgacgcaa tccccgcttt ctggggttcg gtcagtggca ctctgatgtt tttctccacc
     2881 catgccagcg ccttatcacg ctcaatggcg ttaacccggt cgcatttttc cttcgacagc
     2941 ttcatgccag gaataacagg cttaccatcc acccgggtgg ctccacggca gatggtccag
     3001 atacccgcac catcacggta tgctgtggtg tggttacctt ccttttcatc cagaaactgg
     3061 tcgaggattt caggcgcagg cgcacctgcg gcaatcagcg ccagaacggc agccgacagg
     3121 ccgtatctga tttttgcgtt catggatatt tatcagggtt tatcgatttc aaatccctgg
     3181 atatgttaag tcttcaggcc agcggtggag tcttcagaga acccgtaatt attcccggta
     3241 gttttcctct gtaggttatc aacacatcct gcgcctctaa aattacggga cgcttttccg
     3301 gtaacggacc atccccttca cataacccgg cagcaacatc catgaaaaac tgcttcgcct
     3361 gctttttcgc ctcagcttcg taaaactcca gcgtggcacc ttcagtacgg tcaagactaa
     3421 tcgccacatg tggcaacaac aacgacggat gcccgccaat ttcaagtgcc acagtaacag
     3481 caatcttatc cgggtaatta tttatctctt taacaaccag ttcgtatttt ttcttcatcg
     3541 ctttagtctc cccgcgccgt cttacggcgg tcctccctga ttttgaaata caggttagtc
     3601 agatacgtca gcaggccaaa cagcagactc cccagtacac ctattgccac ccactgggac
     3661 ggagagactt tgtccagcag ttgcagtaac cagtatcccg tccctaccgc tgacgtggtg
     3721 tatgacacac c
//
LOCUS       DAZFKM010000259         3695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFKM010000259 DAZFKM010000000
VERSION     DAZFKM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..173)
                     /locus_tag="ACHKAY_005144"
     CDS             complement(<1..173)
                     /locus_tag="ACHKAY_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703625.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTV"
     gene            complement(163..342)
                     /locus_tag="ACHKAY_005145"
     CDS             complement(163..342)
                     /locus_tag="ACHKAY_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5703626.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(518..1069)
                     /gene="ymfL"
                     /locus_tag="ACHKAY_005146"
     CDS             complement(518..1069)
                     /gene="ymfL"
                     /locus_tag="ACHKAY_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO5703627.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGAVACRDCGETNA"
     gene            complement(1062..1322)
                     /locus_tag="ACHKAY_005147"
     CDS             complement(1062..1322)
                     /locus_tag="ACHKAY_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703628.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            1420..2112
                     /locus_tag="ACHKAY_005148"
     CDS             1420..2112
                     /locus_tag="ACHKAY_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO5703629.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(2432..2947)
                     /locus_tag="ACHKAY_005149"
     CDS             complement(2432..2947)
                     /locus_tag="ACHKAY_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703630.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            3092..3403
                     /locus_tag="ACHKAY_005150"
                     /pseudo
     CDS             3092..3403
                     /locus_tag="ACHKAY_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3418..>3695
                     /gene="yfdP"
                     /locus_tag="ACHKAY_005151"
     CDS             3418..>3695
                     /gene="yfdP"
                     /locus_tag="ACHKAY_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO5703631.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGR"
BASE COUNT          999 a          901 c          827 g          968 t
ORIGIN      
        1 ctgacggtac tcatccccgc gccaatctga cgggcaatag tttcaattga tggccagcac
       61 acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct ggataatttc
      121 atgcctgatg cagcgcaacc atcccataca tagccggtta atttagtgct catgaccgac
      181 ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact catgctcata
      241 gccttcgcgg aggtagataa ctcgttgtgt ttccggctcc caacgaatga ctctgacggg
      301 tactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt ctccttctgc
      361 cggttaaaat cacccacagc ccactctgca aagctgtggg ttacaattac cctgccacct
      421 ggtacattaa ctgcatagca atactccacc ttcgcttttc cacccggtac aggaagcgca
      481 atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt tctccacagt
      541 cacgacacgc cacggcgccc ggagctgcac actcgcgggc gtcattactt tctgaaatgc
      601 aaaaaatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag aggtacagct
      661 cgtcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcaatt gctgaacgaa
      721 tctgttttga ataactgccg atctgttcaa tgacctccag caggcgttgg ttgatatcgg
      781 cgttgtccac atcctcgaca tcaggaagag acacaaagac gccatttgca gactgcgcca
      841 cagcgtcagc aatgaagtga gttccaccag cacgttgcaa aatcattgcc catcccagcg
      901 ggaaaatctg atcgccatcg gcacgaaggc ggttaaataa tgcgttctct gttacatcca
      961 gccagtcagc agcttcagcg tacccccccg gcaacgctgc gatagttttt ctgacagctt
     1021 tcacgtacca ctcaggctgt ttttctactt tccagtgatg cttacccacg gttcacctcc
     1081 tgttcctgtg gtttaaaccc attctggttt tggctagatt gaaaacgtgc cggataaaga
     1141 atctgcattt cgctgacttc acccttaaaa aaattggcta aacgttctgc aagctcgata
     1201 gatggaatct gctccagcct ctcaatacga ctcaacgtcg ctggattgac ttgaacaccc
     1261 gcagcaacat gctgcaaagt gaaaccatgc gccttacgca catttcgtaa tggtgattgc
     1321 atacgtcctc caaatattgc gcgttatgca tgttatttca cgcaattatt ttgcgcaagt
     1381 tgatttgctt atcacgcaat aaagaaatgt aataaacgca tgaacatagg aaaccgagtc
     1441 agacaacttc gccaagcgaa gaacatgaaa atcgccgatc tcgctgaagc gataggagta
     1501 gatgcggcga acatctcgcg cttagaaacg ggtaagcaaa aacaatttac cgaacaaaca
     1561 ctgagtaata ttgccaagag cttaggtgtt gatattgctg atctctttac ctctgcccac
     1621 aaaagtaata ctgtatataa aaacagtaat aatgaggatg ttgcgcaggt gaaggatgtg
     1681 ttccgtattg aaatgctgga tatcagtgcc agtgcgggaa atggccttat ccagggcggt
     1741 gatgtcattg atgtgattca tgccatcgaa tacagaactg ataatgctgt atcaatgttc
     1801 ggcggacgac cagccaatca catcaaagtt atcaacgttc gtggggacag tatgtgtcca
     1861 accattgagc caggagatct catcttcgtt gatgtcagca tcaatcagtt tgatggtgat
     1921 ggtatatatg tctttggttt tgatgacaaa atatacgtta aaagacttca aatgattcct
     1981 gacaaactgc tggtgatttc tgataaccag atttaccgtg aatggggaat tactagcgaa
     2041 aacgaacacc gattcatggt ctttggaaag gtcttaatca gtcagtcgca aacccttaag
     2101 agacataatt aacctcaata tcccatccat cggccaccga aaggtggctt tttattacct
     2161 ataaatttgc atatcacgca aatatcactt gcatatctcg caatttaatt ttatcttttg
     2221 ttccagacca actacaggat tacaacaaaa tctggttgca acacggtgca tgtgtcgtaa
     2281 gcagtcagta aatgtcaaaa acgaacaggc aggacgccca cgaagtagcc gcctggggca
     2341 tatgaagtcc aggatgattc gttagcaaca aaaaagcgcc ctacaggacg cttagctctt
     2401 taacaatctg gatatccaca acagtagtaa tctacagatt gccgttaagt tttctggaca
     2461 actcctcaat ggatggaggc gatacgtaat ccggattttt attcatcaga aacttatttt
     2521 cacagtggag gcacctgctt ttatgaaaaa gctcatcttc gctaaccggg aatggttgaa
     2581 gtatcgatac tatcttttgt ccaaaacatt ttgggcaaag atgcatggtt atgctgccac
     2641 cgttcacgat tacctccttc gagtatacaa aagtacccga ctcaagttgg ttaaggatat
     2701 agccttccgt ctgagcctca aagttttcga attctgcaat tttagctttg agagaagcat
     2761 ttatttcttg ataagagccc accagttcaa cgagagacac gcattcgcgc tgaatagacg
     2821 caagctttga gttcagctca ccaatagccg catttacttc agcttgagtt tttgcctcgt
     2881 tcattagttt tgcaatctgg gctgtttcac gaatagccgt cattgctgcc gttaattcag
     2941 cgatcacatt gaatactctt attgttgttg gggatatcca gattaaccga atccttgttg
     3001 ttggggaata accaggtcca cctcgcctga tgtggctaaa agcaggcaca taacagctaa
     3061 gtattttcaa ccagagagaa tccttagcgt tgtggtgaat gcggctcagc gcacgcgggt
     3121 taaggttgag gctgacagtc gaccttctgt ggatacccac ccgcctggtg tgcaaccttc
     3181 gccaggcacc gggaggcacc cggcaccaca actttatgct gtgtgtagtc ctggcggtac
     3241 cagtttgtac ccttgcttcc ggctggtacc gtcctttttt gcaaaacaga gaagagcatc
     3301 accggacgac gggctcataa cccaatccat ccgggcggct gccaccgcag gtgttcttct
     3361 ctgttttgtg gagaaaccaa ccgaccttgc aaggtcgata tgatgaggag cagcaaaatg
     3421 gctagcgaac gcagtactga tgtgcaggca tttatcgggg agctggacgg cggcgtattt
     3481 gaaaccaaaa tcggcgcagt tctcagtgaa gtcgcttccg gtgtgatgaa cacgaaaacc
     3541 aaagggaagg tctcgctcaa cctggaaatc gaaccgtttg atgagaaccg tgtgaaaatc
     3601 aaacacaaac tctcatatgt tcgcccgact aaccgcggga aaatttccga agaagacacc
     3661 accgaaacgc cgatgtatgt caatcgcggt ggtcg
//
LOCUS       DAZFKM010000260         3667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFKM010000260 DAZFKM010000000
VERSION     DAZFKM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            156..1259
                     /gene="proB"
                     /locus_tag="ACHKAY_005152"
     CDS             156..1259
                     /gene="proB"
                     /locus_tag="ACHKAY_005152"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO5703632.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1271..2524
                     /gene="proA"
                     /locus_tag="ACHKAY_005153"
     CDS             1271..2524
                     /gene="proA"
                     /locus_tag="ACHKAY_005153"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO5703633.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2639..2714
                     /locus_tag="ACHKAY_005154"
     tRNA            2639..2714
                     /locus_tag="ACHKAY_005154"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2672..2674,aa:Thr,seq:cgt)
     gene            3319..3477
                     /locus_tag="ACHKAY_005155"
     CDS             3319..3477
                     /locus_tag="ACHKAY_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703634.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          924 a          873 c          986 g          884 t
ORIGIN      
        1 aaatctgtaa taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac
       61 gccatgcttt gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg
      121 atatcatttt tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg
      181 taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg
      241 ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga
      301 cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta
      361 tcgcctcgaa acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac
      421 agctgttttc gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg
      481 aagaccgtga acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata
      541 tcgttccggt aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata
      601 acgataacct ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga
      661 ccgatcaaaa aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta
      721 aagatgttta cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc
      781 tcggaactgg cggcatgagt accaaattgc aggctgctga cgtggcttgc cgtgcgggta
      841 tcgacaccat tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca
      901 tttccgtcgg tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga
      961 ttttcggtgc gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc
     1021 ttgaacgcgg cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc
     1081 gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga tatcgcccac ggcgtcagtc
     1141 gttacaacag cgatgcatta cgccgtattg ccgggcacca ctcgcaagaa attgatgcaa
     1201 tactgggata tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag
     1261 gagcaggctg atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc
     1321 gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc
     1381 acaaagcgaa atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gtgccaatgg
     1441 ccttggcgaa gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc
     1501 cgatgatgtg cgccaggtgt gtaacctcgc cgatccggtg gggcaggtaa tcgatggcag
     1561 cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt
     1621 gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg
     1681 taatgcggtg atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc
     1741 ggtgattcag gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga
     1801 taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct
     1861 gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt
     1921 gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga
     1981 agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga
     2041 aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat
     2101 ggcggaaagc ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg
     2161 ccctgcgaag gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga
     2221 tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg
     2281 cacacaacac tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa
     2341 cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca
     2401 gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat
     2461 ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc
     2521 gtaaataaaa ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg
     2581 cccggttagt tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc
     2641 cgatatagct cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc
     2701 tattatcggc accattaaga aaatcaataa cttagcttaa cttctctaag aaattgtatc
     2761 ttctgttatc acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat
     2821 gggcgagtag ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc
     2881 accgtctcta aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc
     2941 caatgatgct acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag
     3001 ttttaagtgc atagtggatt aaagcccacc tcttcaaaac ctaacttgat cgggaaagaa
     3061 tcaagctcta gcacggtttc gtccaccgga gtacaacggg tgcctgataa gagaagaact
     3121 gctaagactt catagctttt gtgaaaaagc cctttttagt ggatacgcct acggcgctta
     3181 ttttttggta taaccatcaa aggcgcttta tggctcctat tgtcttgaaa atgtatcttt
     3241 acttgtattg tgttttatgc gtagtctccc tgtgtagtat gtaacttgtt aattttcata
     3301 tgaatacaga ggcacacgat gaaaaacatt gcagctatca aacgtaataa ccgcaagatt
     3361 cacgctcgta agttcctgtc tacgccagag ggaaaagcct ggctagagcg aaagcaacga
     3421 gagaacgaag aaagaaaact ccttagtgag ttgaaatggc ttagagagga tttctgaaaa
     3481 tctccagaac gcaacaggac gcattctaac gctgttttca ttgcttggca tagggatgta
     3541 taggtgatgt gtgtttaatg cgtatgtgag cttgttttgt gccttatttt gcattttcat
     3601 aatgcagaag agaacacgga aaaccttaaa attaattatt ggggtattta aggctatttt
     3661 caggtta
//
LOCUS       DAZFKM010000261         3659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFKM010000261 DAZFKM010000000
VERSION     DAZFKM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            83..364
                     /locus_tag="ACHKAY_005156"
     CDS             83..364
                     /locus_tag="ACHKAY_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5703635.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            361..588
                     /locus_tag="ACHKAY_005157"
     CDS             361..588
                     /locus_tag="ACHKAY_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703636.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            581..892
                     /locus_tag="ACHKAY_005158"
     CDS             581..892
                     /locus_tag="ACHKAY_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703637.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            1020..1238
                     /locus_tag="ACHKAY_005159"
     CDS             1020..1238
                     /locus_tag="ACHKAY_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5703638.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            1240..1797
                     /locus_tag="ACHKAY_005160"
     CDS             1240..1797
                     /locus_tag="ACHKAY_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5703639.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            2088..2243
                     /locus_tag="ACHKAY_005161"
     CDS             2088..2243
                     /locus_tag="ACHKAY_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5703640.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            2363..2707
                     /locus_tag="ACHKAY_005162"
     CDS             2363..2707
                     /locus_tag="ACHKAY_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO5703641.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            2829..3101
                     /locus_tag="ACHKAY_005163"
     CDS             2829..3101
                     /locus_tag="ACHKAY_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703642.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            3103..>3659
                     /locus_tag="ACHKAY_005164"
     CDS             3103..>3659
                     /locus_tag="ACHKAY_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5703643.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          973 a          784 c         1025 g          877 t
ORIGIN      
        1 gccgggatat tctccgggat attaccgcca ccagagaaca gcagcggtga gtgggtggaa
       61 gacgtggtgc cgggcggaaa tcatgatact ccggcagtgt gcgggaacga tgaaggtaaa
      121 aagcgttggc gcacttatcg gacgaactga agcggcagtg agaacgaagg cacgggagct
      181 gggcatcagc atgatgttac gtggtgattt tcacccgtcg gcaaaatatt ctcagcgtga
      241 tattgagctg gcgcggcaac tgcatcagag aggcatgcaa agaagggaaa ttgccagaaa
      301 attaggcatg ccgctgcgca tagtgaataa ctacgtttat ttcgacagga gggtgtctgc
      361 gtgaaaatcc tgtatcagga ttacggcccg gtggggcagg tggttatcag cagtactgta
      421 atggagtttc ggaagcataa ccgtgtggtg gatgctgtgc tgttaacctg tccggggata
      481 tcggcgagtc gtgcaggtgt gtttattatg aagacgaaat tatatggcag taaggcgtgg
      541 ataaagaagg cgtatcgtgt agcgttgcag gaggttaaca gtgagtgaaa ttaaagaaat
      601 gccggtagtt cgtgacggat atggctactg gacacatcct gaatatgaaa aattctgtga
      661 tggtcgggaa tatatttcaa cggaagagtt taacgcctgg atggaggaaa ataatcttca
      721 atacgtcctc tgcttcagag atgaaggatg tgctgacctt gatgcgtgtg atgctgatat
      781 ttctgcatgg gaaccggaac gaccagaggg cgatggctgg tttattggtt ccattcatga
      841 tacggaagat ggcccggttt gtgtctggtt gcgaaataag gctgaagcat aaaggcgata
      901 aaccaactaa caactaaata ctgaagattt aaatcagaaa cgatttttat taaatcctta
      961 accggaggga ttcctgcacc ctcagaacat caggaggccg cccgaaaggg cggtaagaaa
     1021 tgaaacatta tttagaaaaa aattacccac gaaagagcag aacaacagag tttctgtttt
     1081 tcattctgtt tatagtgttg atgataccga tatccccgct attactggtc tggataattg
     1141 gaaggacatt tgaaccagtt attgagctat ataccgatgt gacatgggaa tcattcagcg
     1201 cactgcacaa taaaattaat ccgtataagg aaaactgata tgagcactat taccagagaa
     1261 cgcgcggaga ttaaatcata catcacaggc ttcctgagcg actcggcgca cgataacaag
     1321 tcttcagaca gcctgctggc taatgtgttt cgtatcgcgc tggcatcact ggaagcagag
     1381 ccgatagcaa tggtagtgcc tgatgaaatg gatttgctta cctgccatct cgacggtgta
     1441 actaaaacat atgctgatgg ctggaacgcc tgccgcgtcg ccatgcttca ggccggaaac
     1501 tttcgggaaa ataagaattc gtcaaccaac aattttcggg aaatctcgga aacgtcaacc
     1561 agatctccga taactctgga tggctggata agctgtactg agcgaatgcc tgaaaagagc
     1621 cagaacgtgc ttatttcgat gaatatcgat agcgaggctg ggccattaat atattccgca
     1681 cgctatctcg gaggcacgtt ccggcgcgga ggtatagcag ttagtccggg taatgatctt
     1741 aggcaagcaa cccactggat gtcgctacca gaaccgccgc aggaggtgaa tcaatgacct
     1801 ggcctgaagc attcacaacg gtaggaattg cgatggcggt ggcgctggtg gtgtattcga
     1861 tttgccgctg gggataaaaa cggtttgcgg gaaaaggata gttaagtaga attgcagcgg
     1921 gtgcttgagg ctatctgcct cgggcatgaa caccaacggc agatagagaa aagccccagt
     1981 taacattacg cgtcctgcaa gacgcttaac attaatctga ggctcaatcc atgctgaaca
     2041 catgtaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     2101 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     2161 cgaggaaaga cctctgcgag gtgcgaatcc gaaccggtca gacggaggtc gctgtcttcg
     2221 tagactacga atctgagaag taagagacca ggcgggggag taatctcccg ccacctctga
     2281 tgtgtcaggc atcctcaacg cacccgcgct ttaccatact gaaaatgctg tttgaatgtt
     2341 catctctgaa agaggactat gaatgaaaaa ggtattgatt gcagcactta tttccggtgt
     2401 gtcttttggc gcttttgcac agcagggtgg tttccagggg ccagaagcag agcgttcaac
     2461 agtagcgcag gcaaaagaac tgaaggatga tgcatgggtt atccttgaag ggagcatcgt
     2521 taaaaaagtg ggtgatgaac gttatgagtt tcgtgacaat agcgggacaa ttgtcacgga
     2581 tattgatgac agcgtatggg ccgggcagaa tgtttctccg aaagacaaag taagaattga
     2641 gggtgaaatt gataaagacc tgagcagtgt tgaagttgat gtaaaggcac tgaaattatt
     2701 aaagtaaccg cccctgcttg ttaagcccgt cttactgacg ggttttctgt ttgtacattc
     2761 cggcgtattg ccttacaatt cgcgcagtca gcctgaacaa ctgacacctg ctgtcaccgg
     2821 agaatccgat gacacaacac ataaaatccc acaattctga agccgacccg gaaattaagc
     2881 aggggaggcg ttttcgtgcg cctcagtatg gctggtttca ctatctgttc tgtacgatcg
     2941 atgaggcaga tatgcttcaa gaggcgtatc tgcgtcgcgg tgtccgtgtg gagcggagtc
     3001 tgaacgctga tcgtctgacc tggaccgttt ctgtatatct tcctgttcgt gcacatctgc
     3061 cacggacaca tgcctgctac cgtcagcgcg tctggaggta atgtgcgggt attacttcga
     3121 cctgttctgg ttccggaact cgggctggtg gtccttaagc ccggtcgtga atcattgcca
     3181 gtttttcatc gcggcagggt gctggtggag ccggaaccga aaaacatgcg ggcgctgcca
     3241 tctggagcgg ttcctgctgt tcgccagccg ctggcggaag ataaatcact gctgccattt
     3301 ttcagcgatg agcgggtgat tcgtgcagct ggcggcgctg gtgcactgtc tgactggtta
     3361 ttacgtcacg tgaaatcctg ccagtggcca cacggcgatt atcatcacag cgaaaccgtt
     3421 attcacagtt acggtgctgg cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgc
     3481 gaccagacct ccgaatcact tgagcaactt actcaacaaa atctgacagc ctggatgatt
     3541 gacgtcatac gccatgtaat gaatggcacg caggagcggg aattatcgct ggctgaatta
     3601 tcctggtggg cagtctgcaa tcaggtggtg gacgcattac ctgaggcagt atcgcgtcg
//
LOCUS       DAZFKM010000262         3645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFKM010000262 DAZFKM010000000
VERSION     DAZFKM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            101..421
                     /locus_tag="ACHKAY_005165"
     CDS             101..421
                     /locus_tag="ACHKAY_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO5703644.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            436..2769
                     /locus_tag="ACHKAY_005166"
     CDS             436..2769
                     /locus_tag="ACHKAY_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO5703645.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRHLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            3353..3481
                     /gene="rhuM"
                     /locus_tag="ACHKAY_005167"
                     /pseudo
     CDS             3353..3481
                     /gene="rhuM"
                     /locus_tag="ACHKAY_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          849 a         1036 c          994 g          766 t
ORIGIN      
        1 ttcgcgcagg gatacgcacg tcttcaggag acgcagatat gccttatcag ttaatgcaac
       61 cggcacggaa tgcagtcatc tgtcacaggg aggaaagcaa atgaaaacac ccttaccgcc
      121 cgtcttacgc gctgcccttt accgtcgcgc tgtcgcctgt gcctggctga ccgtgtgcga
      181 acgtcagcac cgctacccgc atctcaccct tgagtcactg gaggcggcca tcgccgctga
      241 gctggaaggc ttttatctgc gccagcacgg tgaggaaaaa gggcgtcaga tagcctgtgc
      301 cctgctggaa gatttaatgg aatccggccc cctgaaggcc gcgccgtcgc tgtcctttct
      361 cgggctggtt gtgatggatg aactctgtgc ccgtcacata aaagcgccgg tactgcactg
      421 aaggagaaca acaccatgaa aatgaacgta accgccaccg tcagccatgc gctcggccac
      481 tggccgcgta ttctcccggc gctggggatt caggtgctga agaaccatca tcagccctgt
      541 ccggtctgtg gcgggagtga ccgcttccgt tttgatgaca gggaggggcg cggcacctgg
      601 tactgcaatc agtgtggtgc cggtgacggc ctgaaactgg ttgaaaaggt gtttggtgtt
      661 tccccgtccg acgcggcagc aaaggtggct gccgtgaccg gcagcctgcc cccggctgac
      721 ccggcagtga cggccgccgc cgttgctgaa acagacgctg cccggaagaa cgccgccgca
      781 ctggcacaaa ccctgatggc gaaaacccgt cccggaaccg gtaacgccta cctgacccgc
      841 aagggctttc ccggccggga atgccggatg ctgaccggca cacacagagc cggtggcgtg
      901 agctggcgcg ccggtgacct tgtggtgcca ctgtatgacg acagcggcga actggttaac
      961 cttcagttaa tcagtgctga cggccgtaag cgcaccctga aaggcggaca ggtcaggggc
     1021 acctgtcaca cccttgaagg acagaatcag gccggaaaac gtctgtggat agcggaggga
     1081 tacgcgaccg cacttaccgt gcatcacctg accggtgaaa cggtaatggt ggcgctttct
     1141 tccgtgaacc tcctttctct ggccagcctt gcccggcaga agcatccggc ctgtcagatt
     1201 gtccttgccg cagaccgtga cctcagcggt gacggccaga aaaaagccgc cgcagccgca
     1261 gatgcgtgtg aaggtgttgt tgccctgccg ccggtcttcg gtgactggaa tgatgccttc
     1321 acgcagtacg gcggggaagc cacccgtaag gccatttatg atgccatccg gccaccggct
     1381 gaaagcccgt tcgacaccat gagcgaagcg gagttctccg ccatgagtac cagcgaaaag
     1441 gccatgcgta tctatgagca ttacggcgag gcgctcgcgg ttgatgccaa cggccagctt
     1501 ctgtcccgtt atgaaaatgg tgtctggaag gtgctgccac cacaggactt tgcccgggat
     1561 gtggccgggc tgtttcagcg gctgcgcgcg ccgttctcct ccgggaaggt ggcctccgtg
     1621 gttgacaccc tgaagctgat tattccgcag caggaagccc catcccgcca cctgattggc
     1681 tttcgtaacg gcgtgctcga cacgcagaac ggcacgttcc acccgcacag tccgtcacac
     1741 tggatgcgca ccctgtgcga tgtggatttc accccgccgg tggacggtga aacgctggaa
     1801 acccacgctc ccgcgttctg gcgctggctt gaccgtgccg ccggtggccg tgcggaaaaa
     1861 cgcgacgtga ttctggctgc actgtttatg gtgctggcaa accgctacga ctggcagctc
     1921 tttctggagg tgaccggtcc cggcggcagc ggcaaaagta tcatggccga aatagccacc
     1981 ctgctggccg gggaggataa cgccacgtcg gccaccatcg agacgctgga atccccgcgt
     2041 gaacgtgccg cgttaactgg cttctcactg atacgcctgc cggatcagga aaaatggagc
     2101 ggcgacggtg ccggactcaa ggccatcacc ggcggcgatg cggtgtccgt ggacccgaaa
     2161 taccgggatg cgtactccac gcatattccg gcggtaattc tggccgtgaa caataacccg
     2221 atgcgcttca ccgaccgcag cggcggcgtg tcacgcaggc gggtgattat tcacttcccg
     2281 gaacagatag ccccgcagga gcgcgacccg cagcttaagg acaaaatcac ccgcgagctg
     2341 gcggtcatcg tgcgtcacct gatgcagaag ttcagcgacc cgatgctcgc ccggtcactg
     2401 cttcagtccc agcagaactc agacgaggca ttgaacatca aacgggatgc cgacccgacg
     2461 tttgatttta tcggctatct ggaaaccctg ccgcagacca gcggcatgta tatggggaac
     2521 gccagtatca tcccgcgtaa ttaccgtaaa tacctctatc acgcctatct ggcctacatg
     2581 gaggcaaacg gctaccggaa tgtactcagt ctgaaaatgt tcgggctggg gctgccggtg
     2641 atgctgaagg aatacggact gaattacgag aagcgccata ccaaacaggg gatacagacc
     2701 aacctgacgc tgaaagagga aagctacggc gactggctgc caaaatgtga cgaccctgca
     2761 acaacctgac cctcatgact catctgaccg gcatctgccg gtcttttttc atccctgaat
     2821 cccccgaagg tgaacaatcc actgttcacc cttcaccgta tattcacccg ttatcacact
     2881 gaaattaaaa gagaaaaacg aaaggtgaac agtgtgaaca atcaaatcaa aaaaaacttt
     2941 tttcttcctg agtgatttca gtgcggagga ttaatcaccg gtatgagtca caccggcaga
     3001 atgccggagg tgaagaatcg aatgttcacc cttcacccat tattcaccac ctatcaaact
     3061 gaaataaaag gagaaaacag aaaggtgaac agtgtgaaca gttcttccga aaaaaaattt
     3121 ttttcctgag ttaacgtgcg tgaagatacg tgtaacaatt aatcgataga tattatatgt
     3181 ataaggatgt gtataaatga attagaatag aaaaatataa acgatttgaa tcaagtggtt
     3241 atatggcaat tttgactcct gtgatcttcc gccaaaatgc ctcttctgac gttctccaac
     3301 cccttgtaat actgtcaatc cagatatttt ctactgctca atattcaatc ggcttcagga
     3361 atacctggtc taaggttttg tgatggacga tgagcggctg aagaatccgc ctgtcggttc
     3421 ttctgtcctc gaacgcatcc gcgatatgcg cgccagcatg tgccgtgttt atttgcaggt
     3481 gatttgcccc ctgctggctc tcagccaact gtatcgccat taaggtgaag atgttgtttt
     3541 actcgcccca gaacaccctt ttttatacga ctattctatt tctttaaccg ctggcgaaaa
     3601 aaactgacta taaacttcac caataatcag actgccatca agatt
//
LOCUS       DAZFKM010000263         3589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFKM010000263 DAZFKM010000000
VERSION     DAZFKM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            139..780
                     /locus_tag="ACHKAY_005168"
     CDS             139..780
                     /locus_tag="ACHKAY_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5703646.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            811..933
                     /locus_tag="ACHKAY_005169"
                     /pseudo
     CDS             811..933
                     /locus_tag="ACHKAY_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(1721..2155)
                     /gene="uspF"
                     /locus_tag="ACHKAY_005170"
     CDS             complement(1721..2155)
                     /gene="uspF"
                     /locus_tag="ACHKAY_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO5703647.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(2296..3429)
                     /gene="ompN"
                     /locus_tag="ACHKAY_005171"
     CDS             complement(2296..3429)
                     /gene="ompN"
                     /locus_tag="ACHKAY_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO5703648.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1047 a          684 c          802 g         1056 t
ORIGIN      
        1 atgagaaaag atgaatgtca ctttgataca aaaagagaag ctttttgttg taagtgataa
       61 ttaatgatat aaatatagtt ttatgtgctt ttatttttta ctaaagcaaa attaagttat
      121 taaaatggag ggagaggaat gcctgtagat ttaacgcctt atattttacc tggggttagt
      181 tttttgtctg acattcctca agaaaccttg tctgagatac gtaatcagac tattcgtgga
      241 gaagctcaaa taagactggg tgagttgatg gtgtcaatac gacctatgca ggtaaatgga
      301 tattttatgg gaagtcttaa ccaggatggt ttatcgaatg ataatatcca gattggcctt
      361 caatatatag aacatattga acgtacactt aatcatggta gtttgacaag ccgtgaagtt
      421 acagtactgc gtgaaattga gatgctcgaa aatatggatt tgctttctaa ctaccagtta
      481 gaggagttgt tagataaaat tgaagtatgt gcatttaatg tggagcatgc acaattgcaa
      541 gtgccagaga gcttacgaac atgccctgtt acattatgtg aaccagaaga tggggtattc
      601 atgaggaatt caatgaattc aaatgtttgt atgttgtatg ataaaatggc attaatacat
      661 cttgttaaaa caagggcggc tcatcctttg agcagggaat caatcgcagt ttcaatgatt
      721 gtaggaagag ataattgtgc ttttgaccct gacagaggta acttcgtttt aaaaaattaa
      781 gatagcaatg gcgggttaac ttttcgtaaa atgaaattaa ttctcggtac ggcatgtaaa
      841 acgcggcacc tcgtatgcaa gaacgtactg cggttggttg gtgaactttc gatagtgcta
      901 gtattggatg atttccagcc gttaccgatt ttatataact tcagtggaat tattcaacac
      961 acaggcaaaa cggaataagc tgagaaaaaa agcgtgcttt cccaatctgg ggtaaatcac
     1021 gtctgaatga cacctgaaaa caggtggtgg cctcaagaag gcttggaata ttcttcttta
     1081 aatgttatgt aatttattga tttttcgtgt acgattttaa gacatttatt ccaagaaaaa
     1141 tttttaactc tttgattttt cgactctgta tcatcggtct cgaaacccgt agccacgtcg
     1201 tatgcaagaa cgtgccgcgg ttggctggta agcttttcga tagtgcgagt atctaatgat
     1261 ttccagccgg aactgattct attggaaaga aatcatgggc gcaatttaca gatcacagat
     1321 tgattagtta tagattttta ctgtggcaaa aatgggacaa aaggccgcaa aaggggcaaa
     1381 aatggggcaa aaaaagtgta gattattgtg ctttattgta gttgttaatc ctgcctaaca
     1441 tattgaaaaa cataaaagtt attgttcgtc agatagttgc ggtttttact ctgacttgtt
     1501 caggttgtat tgttctttct tactaatttc ttgattttgc gacatttaaa agcgactcaa
     1561 ttcgttatat ggcatcagaa gagtatgcgt catgccggaa cgcccagcat aagaaatctg
     1621 atataaaaac tgtggcgtgt atggtacgga ttagagggga aaatgtcagc acatttgcga
     1681 aatgaatcaa aaagcccgca gcaatgtgcg ggcgttagtg tcagcgcaca accagcacgg
     1741 agcactctgc gtgacgcact acagctgcgg cgttagaacc gagcagataa gtggtgatat
     1801 ccggtcgatg ggaagcaatg atgatcatat gagcggggat cttcttcgcc aattccagaa
     1861 tgcggtcttt tggcgagcct tcctcaacat ggacatgcac tctgtcggtt ggcagtttaa
     1921 attttttaat gatctcttcc agttgcgatt tggcttccgc tttcaggtca tccattgccg
     1981 gtaattctgc ggaatacgct aaacccagag aggcatagta gggcagtgaa ggtattaccg
     2041 tcaggaaatg aacctctgca tcatcaatct ttgcctctgc ctcaacgtgg ctaatcacgc
     2101 gttgagttaa ttctgaatcg gaaatatcga tagggacaag aatcgttctg ttcataaaac
     2161 ctcctgtttt agtatccgca taaagtgtaa cgccagatga cactttttgt gtaatgacgg
     2221 agttcacatt tttaatttag atcaaaggag gaagaataag cagaaaaagc ccgccataac
     2281 agcgggcagg aggatttaga actgataaac cagacctaaa gcgacaatat catcggtaga
     2341 gatgccgttg gcagcgtaga aactgtcatc ttcatccaac aggttgattt tatagtcaac
     2401 ataagtggac atgtttttat tgaaatagta agtcgcgcca acatcggcgt atttaaccag
     2461 atctttatca tcaacacctg ccgggttgtc tgcaccaccg gcagcgtgca ggtcacggcc
     2521 tttagacatc aggaaagaga ctgccggacg cagaccaaaa tcaaactggt actgtgcagt
     2581 gacttcaaag ttctgggttt tgtttgcaac agcataatcg ctgtcgccaa acggggtcat
     2641 attacgcgtt tctgaataca tggttgccag gtaaatattg ttagcatcgt attttagccc
     2701 agcagtccac gcgtctgctt tatcaccacc cgccgcagta tggttaacct ggtcattggt
     2761 gcggtcagaa gaggtgtatg ccgcaccagc gctaaagccc atgcctaaat catatgttgt
     2821 ggaaagaccc cagccgtcac cgttttcatg gcgaacatca cgtccgttgt tggtgccttc
     2881 ctgaccatta ctggcacctt cgttgttacc ttgatactgc accgcgaagt tcagaccatt
     2941 taccagaccg aagaaatcag tattacgata agtcgcgacg ccattggctc gaccagtcat
     3001 aaagttgtct gcattggtat aagagtcacc gccaaattca ggcagcatat cggtccagcc
     3061 ttcgatgtcg tacattacgc cataattacg tccgtaatcg aaagaaccgt aatctgcaaa
     3121 tttcagtccg gcaaatgcca gacgggtcca tgactggttt tttgaagatt cagtgttgtt
     3181 tgcctgaata ttgtattccc attgaccgta gccagtaagt tgatcgttaa tttgggtttc
     3241 gcctttaaaa cccagacgcg catagctctg gtcgccatct ttcgctgaat tatcagaaaa
     3301 ataatgcagg ccatcaactt tgccatacag atctaatttg ttgccgtctt tattataaac
     3361 ttcggctgca tgtgcagcac ctgcggcgag cagggcagga attaaaagtg ccagtacttt
     3421 gcttttcatt gaataaatcc tttagttatt ttatttgcct tttatcccca ttttggggag
     3481 tgaaaacatc ctaaaggaga agttcagtta gccgataaat atcatttgtt acgccttaag
     3541 taaaacctta atcaaataat tctcctgaaa tgataataag tctggtgaa
//
LOCUS       DAZFKM010000264         3499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFKM010000264 DAZFKM010000000
VERSION     DAZFKM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..745
                     /gene="nleG"
                     /locus_tag="ACHKAY_005172"
     CDS             98..745
                     /gene="nleG"
                     /locus_tag="ACHKAY_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO5703649.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(882..1028)
                     /locus_tag="ACHKAY_005173"
     CDS             complement(882..1028)
                     /locus_tag="ACHKAY_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703650.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
     gene            1456..1734
                     /locus_tag="ACHKAY_005174"
     CDS             1456..1734
                     /locus_tag="ACHKAY_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO5703651.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
     gene            2272..2433
                     /locus_tag="ACHKAY_005175"
                     /pseudo
     CDS             2272..2433
                     /locus_tag="ACHKAY_005175"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2417..2482
                     /locus_tag="ACHKAY_005176"
     CDS             2417..2482
                     /locus_tag="ACHKAY_005176"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO5703652.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            2598..2855
                     /locus_tag="ACHKAY_005177"
     CDS             2598..2855
                     /locus_tag="ACHKAY_005177"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5703653.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(2902..3471)
                     /gene="nleF"
                     /locus_tag="ACHKAY_005178"
     CDS             complement(2902..3471)
                     /gene="nleF"
                     /locus_tag="ACHKAY_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO5703654.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
BASE COUNT         1085 a          748 c          660 g         1006 t
ORIGIN      
        1 caaaatttaa ttttttgttt gaatcctatc acatatttag tatcttgact cctgtcacca
       61 atatggttca ataacataga ctaaagaggg agaaaatatg cctgtcatat taaacttttc
      121 gagtgaaaga gtattgtctg aaagtgaact ggaggcttta cgtcatgtcg gaagagttag
      181 tcagagtgaa cagcttgttg tgagaggccg cactatgaga cttcatcata tttcatttat
      241 ggacagtttt agcgtggaac cggtttccgg cggactgttg gatcgcctta gtgcaagagg
      301 gcatcgcctt ctcgcagaaa atctggagat acagcttaac agaggccata cttttttgca
      361 ggcattccgt ttatatatgg aacagagccg tgcaacccca tgtacgcgcc aaaacgtcag
      421 tagtgcaata caaaacaaaa ttaactctca tgcatttact gttagtcatc aagacttttc
      481 ctgtcatgaa caacacctga attgcccgat aacattatgc attcctgaaa ctggtgtttt
      541 tgtcagaaat gcaaaaaact cagaaatatg ttcattatat gaccacaatg cactcactga
      601 acttatccga cgcaacgctc cacaccctct cagccgtgaa ccctttgttc cggaaatgat
      661 tgtcagcaaa gatgaatgcc attttaatct aatagaacaa tatttttgca tattagccac
      721 acaaaatata tgcactagaa tttgactgta tggttcgatt taaatgttgc aaaattaatt
      781 ttactagtta ttgccatcta tttcatactc cctaattctt gatacaatct gtctgtcaaa
      841 atagtagatt agatcgaact tatattacag ctatttagtt tctaaaatgg aatttttaac
      901 gttttatact tcacattaca atctgtacca taagcctcct cagcgaggtt gcctgaatac
      961 cctgtcgtgc ccatttttcc atcggacgca gttgtctgct ctctcgaact gctggcaata
     1021 aaaatcaacc agacagcttg atgtccgtta catgcaggac taatcacgat tggttaccgt
     1081 ttacaaaact tgcgtaacat tggaggagaa cgaataatat tctctagctg gtacgcgttc
     1141 catagcgaaa cgggcaaggt ccaacagcaa gagctgaaac tcagggggat ttatcagtcg
     1201 atttattcgc catggatgaa tcacgctgta cggttatggt agctcttagc gacaaggcat
     1261 aatcatcagt acaccaatgt aacaaccgtt gaaaaagact cattatttta tgaaccagcc
     1321 atcccatttc cagagtggta aatatgattt gttaaagtgt aacagtactc tgcgtagcct
     1381 ttttagattc atagtttaat gtttctaaat acattaaatt gcactataag agaaagacat
     1441 aatgtgagga taaaaatgcc attttcaata aaaagtattt tttcaggaca cacttggcat
     1501 caacctgaaa tatctaggcc tatagcagat aaatcatcta ctaaaaattg tatcttagac
     1561 tcaacaacat gcaatgtaga tggctttaca gtgtttaaca gaagatcctg tagttttgat
     1621 atgcgtcctc ccggaagcgc agacagaact ccacaactga gattatcaat atcagaagtc
     1681 gcatggatgt caaaaataat agagacagag acaaataata caaacaaatc atagctatta
     1741 gcccagaaaa gagttggtac actagccatg aataacttcc taacaacttg attttttagt
     1801 tatttttttc ttcattcttg aaaaaggatg ccccgaaagg gttccagagt tcgattttct
     1861 gcgcttctgc aaataatcaa ggggttacaa agtggttgcc cctttgtttt tagtgctctt
     1921 ggtataccca ttggtacatt tagcgcaata ccatctggta tcacttaaag acacgaaaaa
     1981 caacttttta tctttttgtt tcgctcaaat tagtataaaa agccgaacaa caaacaataa
     2041 aaaaccatta acatcaatag attatgatgg cttcaatcta aataataggc tatataatac
     2101 tgcaagacac aacacatgct gtcactatga gtcaactatt tagatgatat tagtgacctg
     2161 tttcagagca ttagcgcaag gcgattcttt gtcttcttgc actaattttt tatcataaaa
     2221 atgttcctag cactgggcat caatatcgca ggtcagaaag agctcctggg gatgcggctg
     2281 gccgaaaatg aaggggcgaa tttctggttc aatgtgctga ctgaactgaa aaaccgcggt
     2341 ctgaacgata tcctcatcgc ctgtgtgtat ggcctgaaag aattcccgga ggcccgcatc
     2401 cagttatgca tcgtgcatat ggtgcgcaac agcatgcgct tcgtgtcatg gaaggaatac
     2461 aaagccgtca ctcgcgacct gaaagcgatt agcctcccac agaagaggca ggccagcagg
     2521 cactggaagc gtttgctgcg gcctgggact gccgctatcc gcagataagc cggtgctagc
     2581 tgtcaaactg gactaacttg gcgacgtttt tcgcttatcc ggcagatatc cgcaaagtga
     2641 tctacacaac gaacgccatc gagtcgctga acagtgtgat ccggcatgcc atcaggaaac
     2701 gcaaggtgtt cccgacggac gagtcagtaa aaaaagtggt gtggctggca atccaggccg
     2761 cgtcacagaa atggacaatg ccgttaaggg actggcgaat ggcaatgagc cgctttatta
     2821 tcgagtttgg tgaccgtctt gacggtcact tttaagaaaa ggcatttaca cagaatccta
     2881 aacaggctca caggcctcta atcatccaca ttgtaaagat cctttgttgt aagtaagatc
     2941 tggtacccta ataatatcaa aaggattaaa atcatcactg catccttcca cgaggcattt
     3001 cattgctcgt agatcaccat atagctctga agagtgatgc ggacatagca gattataaac
     3061 tttacatttc gaatcttctt tgccttgtct actttcctca atttttactt tcaatgaatg
     3121 cagaaaaaaa agatccctga tataactatc cctgtcctct atcgtcattc ccgccctcag
     3181 cgctccgaca acctcatcag tattttcatt tctcacctca aatacactat ttctcttatc
     3241 catcagccca cttacaaaag cacttagctc ttgaaaaatt tcctcaagct cattattatt
     3301 tctggcacat gaatatatag agaccataac atcaagttta tcttgtaatt caggagaaag
     3361 aaagtgatta tgattaagct cacttactga ttccggagtc gaagggttac ctcttcctga
     3421 tacatacatc catgaataaa gatttgctga agaaccactt gttggtaaca tatcaaaacc
     3481 cccttaacaa ataaatcca
//
LOCUS       DAZFKM010000265         3447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFKM010000265 DAZFKM010000000
VERSION     DAZFKM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(655..852)
                     /locus_tag="ACHKAY_005179"
     CDS             complement(655..852)
                     /locus_tag="ACHKAY_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703655.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(918..1188)
                     /locus_tag="ACHKAY_005180"
                     /pseudo
     CDS             complement(918..1188)
                     /locus_tag="ACHKAY_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1681
                     /locus_tag="ACHKAY_005181"
     CDS             1493..1681
                     /locus_tag="ACHKAY_005181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703656.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            2009..2203
                     /locus_tag="ACHKAY_005182"
     CDS             2009..2203
                     /locus_tag="ACHKAY_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703657.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2546..2674
                     /locus_tag="ACHKAY_005183"
     CDS             2546..2674
                     /locus_tag="ACHKAY_005183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703658.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2984..3319)
                     /locus_tag="ACHKAY_005184"
     CDS             complement(2984..3319)
                     /locus_tag="ACHKAY_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703659.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1032 a          782 c          746 g          887 t
ORIGIN      
        1 agcttatgca caaaaataac caagtatatt atcttctggt agagattcag gatacagttt
       61 gtaggagctt tcttttttga acaatgattt gcaggacagt tatgctaaac tagcggaatc
      121 agtatcacaa caataaatga ctgatcatac ctgaaatttg aattaacagg catcatgaat
      181 gtcaatagtc taagcggtga ctacttatat cgcttgcata aatactaaac actgactaga
      241 aaagtattgg ataaccccgt cactaaaagt aacgcttcgg ttatttgctc ggaatgtatt
      301 aacgccaaga ttgttagccg ttacgcctta tatcactggc gctgacaaat gtttcttttc
      361 taatttactg aaccggcgca gacacgcagg aaagcacgcc ggcaccggtg taatgccagt
      421 tctaatcaat aagaatggaa tgacgaatca gtcaggaagg gataacaacc cgctgctgaa
      481 cggaaggtat cagcaggaag atgaacggga atatcgccag caccaagggc agggccgggt
      541 tccacaatac cagcgctggc attttgcaca ttgccagcgt accggcatta caacacgttt
      601 catgctgctg ccccccagga ttcctcgatt cgttgctaca tccactgctc tacttcactt
      661 ccactgctcc acttcacttt tgtaccagcg gctgctgcgc cccagtttga tgggtttagg
      721 gaaatggcct tcactgatca acttgtaaaa ccatttgtcg gtcataccgg tgaaactggt
      781 gatatagttc atgtcgatca gggaattatc ggcacgcaga aatacgctgt cgtggcttgt
      841 tgtagtcaac atggtcagaa tgtcctgtga gatttatgaa gattgtgcga atgcgggaaa
      901 tctactgagc tgtgctttca gaactggcct gttacgggat accagggatt accggcgggg
      961 taacgggctt ccggatcata cacaccacga ttatcgcgga caaaatcact gaacgcccat
     1021 atcacctctt taagtatgtc ttcgcagtcc ggtaaatgac gatccagcgc cacatcccgg
     1081 gtggtacagc tttgatgcgc ccggtgacac aaagcccgga ttgttccaga catcctgaat
     1141 caaacgcccc agattagggg cgtcgaaata tgcctctctg accattatat tccggcgtat
     1201 aggtagcagg tctgaagtga caatgcgtca cctgacgtta aaagtcaccc aagatgaccc
     1261 cagtgggggc aacaagtata aaaccgtttt ttgattaagt ggtcggatat gaaaagggga
     1321 aggtttatgt gaaaaatacc tgtgttttct ttgaaaacac acaacagtat caggttattt
     1381 atgtttatac agtgcgttcc aaactgacct gaataattac gcaagttgca acaataaaac
     1441 atttttgccg tagtgtgagg tactccggta aattgcttcc caggtgcaga caatgactaa
     1501 cgaagaacta caggcaatcg cattatccat gcttgagcgc cagcgcgaaa aagtacacca
     1561 gcaacgagag tctttgctta acccatctcc attcaccagg ccaaaacgat tggagcttga
     1621 agcattcttg gacaaatacc cacggcggct taaatcaggc aaaaccagac caccaggctg
     1681 atagctcaca gtttgttctc cgattcaata aaacggataa gaaccatcag cgcggtagcg
     1741 taaggcacta cagcgcattg ctttacgagt aataacaatg gatttatcac cagaagacgc
     1801 aaagttttta cgagaactgc aacaccgtat gctcagagaa gccattatcg aacagcaaac
     1861 gggcatgagc gccatagacc cattgcgata caaacgggcc tcatctcctt tagttgacga
     1921 caattccaat gcggataaaa cacgtccacc acactgatta catttttcat tagcacagtg
     1981 gtgtgatgca gcatacgagg aaaaccggat gaatgagaac aaaataaaaa ggctggagca
     2041 actgttacag gcacgccaac gggagtttgc cactaaatgg ggacgtgccg cgctgccaca
     2101 tgaacgccta acctattttc cacttgaaga cctcattaaa aataatggca aaaaaatcag
     2161 gtctgataag aaacacttct tagaatgcaa tgaagacaaa taacaacaat ctgcatattg
     2221 ccaccaacca cagatgtggc atcgttatca gcgcacttga ttttctttat atccactgac
     2281 gagtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagca cactagcatg
     2341 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
     2401 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
     2461 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
     2521 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
     2581 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
     2641 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     2701 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaattgc
     2761 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     2821 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     2881 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     2941 atataaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     3001 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     3061 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     3121 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tcccctttta ccagtgctga
     3181 tttccacatt ccgtccaaca gagcttatag cctttccctg gattatagca ttgtccggct
     3241 gaagttcttt ttgaataata atagaagcac tgctggcaga tccagtccgt ttttcataac
     3301 ccactgtact gataaccata atctaatcag tagaaattga gtcgaaaata agcactactc
     3361 catacaggat aattagaggt cagtttgatt attcacaatt catcatcagc attttctatt
     3421 tctgacgaaa tcaatatgaa aataacc
//
LOCUS       DAZFKM010000266         3430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFKM010000266 DAZFKM010000000
VERSION     DAZFKM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            195..881
                     /locus_tag="ACHKAY_005185"
     CDS             195..881
                     /locus_tag="ACHKAY_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO5703660.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(972..1108)
                     /locus_tag="ACHKAY_005186"
     ncRNA           complement(972..1108)
                     /ncRNA_class="other"
                     /locus_tag="ACHKAY_005186"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1152..1288)
                     /locus_tag="ACHKAY_005187"
     ncRNA           complement(1152..1288)
                     /ncRNA_class="other"
                     /locus_tag="ACHKAY_005187"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            1507..2709
                     /locus_tag="ACHKAY_005188"
     CDS             1507..2709
                     /locus_tag="ACHKAY_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5703661.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(2896..3430)
                     /locus_tag="ACHKAY_005189"
                     /pseudo
     CDS             complement(2896..3430)
                     /locus_tag="ACHKAY_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          922 a          683 c          831 g          994 t
ORIGIN      
        1 attaattctt tgctaatatt gtgtgttgtt atttttttgt ggaaggcaag ccgcaatggc
       61 taaataggga tggttgtaat aatcaaaaca tgatactaac tatatattga tcgttgcatt
      121 gttggcatgt aacacgctgc agcgagtaac attggtaatg aaatataatc aacatggata
      181 ggtgaaaatt aattatgagc tttatggctt ttgtaacttg tttgacaatt gcatttatcg
      241 ccggttttgc tgttgccaga aaattgacac tccgaaaagt tgcctcatcg gtaatcatcg
      301 ctaaatcgca acccgtccct tcatctgcaa cttcccctgc cgctaaggcg tcgacaagga
      361 gctatatacc gcagaaaacc tatcgtgact ctcgttggca agagcgagat gagttatttc
      421 agggaatggt tgccgaaggt attttattgg aaagaaaaga gtggttaatc acgcgttgtg
      481 aaaaaatgta tttcgataat ctggaaaaat ggtttggaca ttattgtcat atccattgcc
      541 aggtctctct ggggcgcttg atcaactttc cagaacaacc tgagtttagc gaagaagaaa
      601 gaaagcgttt ctttgcaatt tataatggca tggcaatgga ttatgttctg gtttcaaaga
      661 agaacaataa aatagtctgt gttattgagc tggatgaccc gagtcatgaa agggcggatc
      721 gcattattcg tgaccgttgg ttggatacaa ttatgactct tgcacgggtg ccttttgtac
      781 gagcttctgt cactaaattg gaggaaattc ctccggtctg ggagactcgt gacaaaattc
      841 gcccattagt cgcggcgaca aagagtgaac cggtgcatta actaagcatc ccggtcgggg
      901 cttctcatcc ccccggtgtg tgcaatatac gaaaaaaagc ccgtaccttc gtacgagctc
      961 ttctttaaag atggcggtga gggggggatt gactcgcttc gctcgccctg cgggcagccc
     1021 gctcactgcg ttcacggtct gtccaactgg ctgtcgccag ttgtcgaacc ccggtcgggg
     1081 cttctcatcc ccccggtgtg tgcaatatac gaaaaaaagc ccgtactttc gtacgagctc
     1141 ttctttaaat atggcggtga gggggggatt gactcgcttc gctcgccctg cgggcagccc
     1201 gctcactgcg ttcacggtct gtccaactgg ctgttgccag ttgtcgaacc ccggtcgggg
     1261 cttctcatcc ccccggtgtg tgaaatatac gaaaaaaaag cccgtacttt cgtacgagct
     1321 cttctttaaa tatggcggtg agggggaccg cctgaaataa atctgactta atccaggggt
     1381 gttatataat ttcattataa acaatgtatt acactttttg ttgtccgata ttatctaatg
     1441 tattcttagt gaagcgagag actaatggca tactggatgg cataccagag tgacgggaat
     1501 tagcgtatgg cagtattgac ggatacgaaa gcaagacata tcaaacctga tgacaaacca
     1561 ttgccccatg ggggaattac cggactgacc cttcatcctt cttcagtaaa ggggcggggg
     1621 aaatgggttt ttcgttatgt aagtccggtg acacaaaaaa gacgtaatgc tggattggga
     1681 acttacccag aggtcagtat tgctgaagct gcacgtactg cccggataat gcgagagcaa
     1741 cttgctgcag gtgatgatcc tctggagatt aaaaaggctg aatctgagaa agtcgctatc
     1801 ccaacatttg ccgatgcagc caggcgtgta catgcagaac tgtctcctgg atgggaaaat
     1861 ccaaagcatg taaggcagtg gttatcgacg cttgagaatt acgcgtttcc tcaactggga
     1921 gcaaaaacgc tggattcgat tacggctgcg gacgtggcag aaacactgcg tccagtctgg
     1981 ttaaccttgt cagaaacggc aagccgggtt aaacagcgca ttcatgttgt tatgcagtgg
     2041 ggatgggcgc acggtttttg tgtagcaaat cctgttgatg tggttgacca tttgcttcct
     2101 cagcagacaa gaggacgtga tgaacaccaa cccgcaatgc cctggaggca gttaccgctt
     2161 tttgtggcga ccagtgtgta taccgatgaa ccttataatg ttacccgcgc actgttatta
     2221 atggtgatac ttacagcaac tcgctcgggc gaagccaggg gaatgcgctg ggctgaaatt
     2281 gattttcata agcgggtatg gactatacct gcagaaagaa tgaaagccag gatacagcat
     2341 cgtgttcctt tatcccggca ggctatttac attctggaaa atatacgtgg cctgcatgat
     2401 gaactggtgt tcccttcacc cagaaagcag cagatccttt ccgatatggt gttgacaagt
     2461 tttctgcgta aaaagaaagc cgtcagtgac attccggggc gagttgccac ggcacatggt
     2521 tttcgctcaa cattcaggga ctggtgtagc gaacaggggt attcgcggga tctggcggaa
     2581 agggcgctcg ctcatacgct gaaaaataag gttgaggcgg catatcatcg tactgatcta
     2641 ctggagcagc gtgtaccgat gatgcaggca tgggcggatt atgtgatgtc tcaaattgtg
     2701 aataaataat agtgaaaatg tatattgtac gatcatgatt tgatatgtga tcgtagcagt
     2761 agtaattaag aaaccgcttt tatgtcaaat gtattgagct gataattatc agatcgtcag
     2821 gaaagcgtat tttgattaac aggggattac ttaacaatcc cctttttata aaaaataatt
     2881 tgcctgttta actcttcact cctgctgaga ctgaattatt cctagtgcgt cataaatcag
     2941 tcttctgcgt agtttattgt ttttatctgc accatataac ataaatatat tttcaacttt
     3001 ttcttcaggt gttctgagat aatggataag aaaggttatc attccaacac aatggaataa
     3061 agtgaatcca tcaaacaggc ttatcaggtt tccttttttt cccgtgttgt aagtattttt
     3121 tcgcgggtaa gaatatttgc cagttcatgg ggggctgata tgtttctgct gtctataatg
     3181 tcatttattt taccaagggc tatatcaact tcctgtaata ggttatttat catagctgag
     3241 tgaatgttat tggtttcatg ttcctgacag tttatgaact tataaatcat tgagttatac
     3301 agaaatatta ctcttcctaa taaatatgtg tttgtgtcat tttgaagaca ggactctggg
     3361 tattcgtgat ccagtgaaat aaggttaatg gactcgccct gataagtcat aaaataattg
     3421 atgtcattta
//
LOCUS       DAZFKM010000267         3427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFKM010000267 DAZFKM010000000
VERSION     DAZFKM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(484..2832)
                     /gene="nleL"
                     /locus_tag="ACHKAY_005190"
     CDS             complement(484..2832)
                     /gene="nleL"
                     /locus_tag="ACHKAY_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO5703662.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMEN
                     GSLLQAGMYFERHPGAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVT
                     QQSDFGLCDGSGKPDWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWM
                     SFFLYKDGEVQNTFGYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKER
                     FIEALNSNKSDYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQ
                     AEILFCLGGGFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDI
                     LDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
     gene            complement(2852..2941)
                     /locus_tag="ACHKAY_005191"
     CDS             complement(2852..2941)
                     /locus_tag="ACHKAY_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703663.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            complement(2954..3046)
                     /locus_tag="ACHKAY_005192"
                     /pseudo
     CDS             complement(2954..3046)
                     /locus_tag="ACHKAY_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(3048..3317)
                     /locus_tag="ACHKAY_005193"
     CDS             complement(3048..3317)
                     /locus_tag="ACHKAY_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703664.1"
                     /translation="MNILKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(3319..>3427)
                     /locus_tag="ACHKAY_005194"
     CDS             complement(3319..>3427)
                     /locus_tag="ACHKAY_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703665.1"
                     /translation="VQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT         1135 a          765 c          503 g         1024 t
ORIGIN      
        1 attaatctga atatcaattc ataaaatatg attctccacg atttgcccag aaataacgaa
       61 cataattttc gttgtaatta aaatcatatt tcatctatag tgaccattca cagcaaaaca
      121 aaatcaatta tcacattcaa aaaaaatact gacaacaaag atactcttca gacaaagttg
      181 tcatatgtaa attttccctt aaaacgttgc tcaaaaaaaa tataaaaatc atatgcccta
      241 atcaacatcc tcaagcacta cgatatatgc agtatcctat tcatatatta ccaccatatc
      301 agacaaatcg aatcacaacg agttctatcg tactctttca tcttcgacac tgtaaatttt
      361 aagaacattc ggctcatgcc actaaaccaa gacataataa caacggagca ataaatgttc
      421 ccactgaaca tgtgcaaacc taaaaagaca gtgttacaat aatattcatc catggtctct
      481 aaatcaacgc cacgcaacag gataatacaa tgaaaatatt tcaggaaaag attcttttgc
      541 atgctccgtt agcatatcag tcagcacagc ggtacaagta aaaacattat ttcttccttg
      601 tagcctgtct aaaatatcat tgtaataata aactgagcca aatacctgag gatctgtttt
      661 ataagcttgt tcaatcaatc catttgcata tctccgtaga atctcaggag aatcatactc
      721 tgtaccaaac atatcactcg atgagtattt acagaaaccc ccccctaaac aaaataatat
      781 ttcagcctgt tttcttagtg gcatatcttt caatacatgg ctccccataa tcatatctac
      841 atgctgagcg ttcagttccc aaccatcaag aaagggattc cagacacagg caagtttcct
      901 ttgctgatca tcagcaatca ttttataatc tgatttattg gaattaagtg cctcaataaa
      961 tctctctttg agttcattat cagaaatgag tatatccaga ataccagaaa cgaaattctg
     1021 ggaaaaagct ttatgataag gaatactgaa aattggaaat gattcagaaa aaagattgct
     1081 caatgaatac ccaaatgtat tttgaacttc tccatcctta tataaaaaaa aggacatcca
     1141 gttagttgat gtattaggag ataacatatc aaccatatga ctcaaagaac acatcattgc
     1201 cataccatca tcctgtgatg ataacaaaac ccagttataa gccaaatcgg aatcatcatc
     1261 ccagtcaggc ttcccagagc catcgcacaa tccaaaatca ctctgctgtg tgacaggata
     1321 aactttttca ttttttaaat atacttcata gagttcccta aatcgttcct gcattccatc
     1381 acgccgtgaa ccattcatta caatttgtat aaaggaacta ttataagata ccatcgcacc
     1441 tggatgtcgc tcaaaataca tccctgcctg caataaacta ccattctcca tatacatcat
     1501 tattgaatta ttaaaccaag acattatagt atcaaaaaag tcatcacata taggctcgag
     1561 ccattgctgt atatttgcgt tgttcaaata aggatgaata ctaaatgaat cagccaaaga
     1621 catcttaaca tagggccgga acgccgctaa ttcgtcaaga gatatttttt tgataagttc
     1681 ttcagcggcc cttactttat catggttata cttatcatca gatgtattca tagtcaataa
     1741 aattgattta tttgattttg tatagttatc caacacagat aatatatttt cctgtatgtc
     1801 agatagatct atgtaacacc ctcctagatt aatggtatta cttttttcac taaagagctc
     1861 ctttacatca cctaagatag caccacttaa aaccattcca ggaattataa tagctcctgt
     1921 taaatcagcc tcgttcaaaa ttggaggata ttgtatatat gaagatcctt taaatgatgc
     1981 atttttaaga ttggaattgc tgaaattaga gttttcaaac aaagccctaa taatacaatt
     2041 agataaatta gcataacaaa aattacattc attcaaaatc gagtttttaa aagaacagtt
     2101 ttccaataca gcctgagaca aatcactcat cctgagatct gtatcatcaa gcactgctcc
     2161 agaaaaattt actgatgata aattcagccc tacaagactt agacctgaca agtcacatcc
     2221 agagtaattc aattcttcag cagactcttc acccgttcgg ttagccgaca accaaattaa
     2281 gtcagcagta agctcagctt tgctcaggca ggcacggcct tgactgacat catatgcaag
     2341 gaattgacat tgcgattcat ttaatgaatt acagccattt aaatatacgg aatttctgag
     2401 tgcctcagga aaggaacctt ctatatgttt caaagaacta caatggcata aagataaatt
     2461 aatgacattt tcaggtatgc tgcaatttat aacctctaat gatgagcatc caaccatact
     2521 taatgaagat aagttggggg gtaagcagtt tattgattta agctctgtac atccattcaa
     2581 aaccagttct ttcagagaac ctggaagcaa gtctggtaat gttgttattg gctcactgat
     2641 tgataaagtc tctccattag tacttataac atcaagtatt tttgatgcaa cctcatgacg
     2701 attttcgcta aattctccct cagcgcacca cttttcgagg gcaacttcaa catcctcgtt
     2761 agatggtgaa tctacaggac tttcaaaaga tattactcca gaatttacag agatatttgt
     2821 agtgggcagc atttatctca ctccttttta cttagtccac taaagtaaaa ttagaaaaca
     2881 caactgccgg gacaactttc tgtccctgta cccacaaata agatgtcaga tttatcggca
     2941 ttaatttaac tccctattga attatctcct cttttttatc taacaaatat tccccggaca
     3001 tgacaacaaa aaccggagcc ggactccggt tttgtgaagc tgtcgggtta cttcatccct
     3061 ccaatatttt cccacgtccc gtcagcacgc aggatttgca gcggtcttac cacgcactgt
     3121 atctgctttt tatccgcatc cagtatcacc acctgcgtga ttaccctgtc ctgctccggg
     3181 ataatgccat tctcatctga ctccaggatg tctgccggcc ccagtcgcag ctgtgctgta
     3241 agtaactccc cgttttcacg gtcatcatgc tttccgcaac cgcacagacg ctgcataagt
     3301 ttttttagta tattcatgtc attctcctgt tctgcctgta tcactgccca cttcatccag
     3361 ccccttgaca tcctgccacg gcccgtcgcc aaatctgacc tgcaaatgct gaaaaaaacc
     3421 ctgaacc
//
LOCUS       DAZFKM010000268         3353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFKM010000268 DAZFKM010000000
VERSION     DAZFKM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            217..1737
                     /locus_tag="ACHKAY_005195"
     CDS             217..1737
                     /locus_tag="ACHKAY_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO5703666.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1762..2100)
                     /gene="maoP"
                     /locus_tag="ACHKAY_005196"
     CDS             complement(1762..2100)
                     /gene="maoP"
                     /locus_tag="ACHKAY_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO5703667.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2219..3058
                     /gene="hdfR"
                     /locus_tag="ACHKAY_005197"
     CDS             2219..3058
                     /gene="hdfR"
                     /locus_tag="ACHKAY_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO5703668.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3154..3229)
                     /locus_tag="ACHKAY_005198"
     tRNA            complement(3154..3229)
                     /locus_tag="ACHKAY_005198"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3194..3196),aa:Trp,seq:cca)
     gene            complement(3238..3314)
                     /locus_tag="ACHKAY_005199"
     tRNA            complement(3238..3314)
                     /locus_tag="ACHKAY_005199"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3278..3280),aa:Asp,seq:gtc)
BASE COUNT          902 a          822 c          820 g          809 t
ORIGIN      
        1 aaaatagtga gtgaaaaaaa ttcaatttca gtatgaatgt tgttcccttc ctcgtagttc
       61 atcctttgtc cgctgcattt ttgcgagcat cattccaccg ccagaacaac tcaacttcac
      121 ttgcacaaat aaacaggaat cagccactaa aattcacgaa ttagcgggct acagcagcga
      181 tatcttatgc acataatagt gactcaagga gggcttatgt cactgtcaat tgttcatacc
      241 cgcgcagccc tgggagtaaa tgcgccccca atcactgttg aggtacatat cagtaaaggt
      301 ctacccggct taacgatggt gggcttacca gaaacaactg taaaagaagc tcgcgatcgc
      361 gtgcgcagcg ccattatcaa tagcggatat gaatatccgg cgaaaaaaat caccatcaac
      421 ctggctccag ccgatctgcc aaaagaaggg ggacgatatg atttacctat cgccattgcg
      481 ttgctggcgg cctcagaaca gcttacagcc aataagttag atgaatatga attagtcgga
      541 gaactggcgc ttacaggcgc tctgcgtggc gttcccggcg caatctccag tgcaactgaa
      601 gctattaagt cgggcagaaa aattatcgtc gcgaaagata acgaagatga agtggggcta
      661 attaacggtg aaggatgcct gatagccgat catctgcagg ctgtctgtgc gtttctggaa
      721 ggtaagcacg ctctcgaacg cccgaaacca actgatgcag tatcccgggc gctacaacat
      781 gatctcagtg atgttgtcgg tcaggagcaa ggaaagcgag gactggaaat taccgccgct
      841 ggcgggcaca accttttact gattgggccg ccgggaacag gtaaaacaat gctcgccagc
      901 cgtattaatg gcctgttgcc agatttaagc aatgaagagg cactggagag cgctgcgata
      961 ttaagtctgg taaatgctga atcagtacaa aaacaatggc ggcagcgccc gttccgctca
     1021 ccgcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc agggccaggt
     1081 gaaatttcgt tggcgcataa cggcgtgctt tttcttgatg agctacctga atttgaacgg
     1141 cgtacactgg atgccttgcg agagccgatt gaatccgggc agatccatct ttcacgcaca
     1201 cgagccaaaa taacctatcc agcccgtttc cagcttgttg cggcgatgaa tcccagccct
     1261 accggacatt atcagggaaa ccataaccgc tgcacgccag aacagacatt acgttatctc
     1321 aaccggctct cggggccctt tctcgaccgc ttcgatctct cactggagat cccattacca
     1381 ccccccggca ttttgagtaa aacggtagtg ccgggagaaa gcagcgccac cgttaaacaa
     1441 cgcgtaatgg ccgccagaga gcgccaattt aagcggcaga ataaactgaa tgcctggctg
     1501 gatagtccgg aaatacgcca attctgcaaa cttgagagcg aagatgcgat gtggttggaa
     1561 ggaacactga tccatctggg gttatcgatt cgtgcctggc agcggttatt gaaagttgca
     1621 cgaaccattg ctgatattga tcagtctgac attatcacac gtcagcattt gcaggaggca
     1681 gttagctatc gtgcgattga ccgtttgctc atccatctgc aaaaactact gacataaaaa
     1741 aagggcattt cgcccttttt attaatcatc agaatcggtg tagtcttcag caccttcaac
     1801 ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga cgcttaatac gcgtcatata
     1861 cttggaccac acgcgttctg cttctgtcac tggctcacgt tcgccacggc atactgctac
     1921 gaagagtttc tcttcctcgg taaccggctc gcgtttgcca agatccaact cattgaaggc
     1981 ataaccatga cgctcaagca gttgtgcctc tttgatggtg aaatcaccat gacgagagaa
     2041 tccacgtgga taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat
     2101 cctgcacgct cctaattctt tgaccgagct agttatggcg cggagtatta gttacgcttg
     2161 acagagtgta aaacaaaaca tttaaatcat aacgacaaat aattttgcgg agagcactgt
     2221 ggatacggaa ttgttaaaaa ctttcctgga agttagccga acgcgtcact ttggtcgagc
     2281 ggctgaatcg ctctatctga cccagtcagc agtgagcttt cgaatcagac aactggaaaa
     2341 tcaactgggt gtgaaccttt tcacccgcca cagaaacaat atccgtttaa ccgctgccgg
     2401 tgaaaaacta ctgccttatg cagaaacgct catgagcacc tggcaggccg cccgcaagga
     2461 ggtggcgcat acctcacgac ataacgagtt ttctatcggt gccagcgcct cattgtggga
     2521 atgtatgctt aatcagtggc tgggacgttt gtatcaaaat caggatgccc atacaggctt
     2581 acagttcgaa gcgcgaattg cccaacggca gtctctggta aaacagctgc atgaacgcca
     2641 gcttgatctt cttattacca ctgaagcgcc caaaatggac gaatttagta gtcagttgct
     2701 gggatatttc actttagcgc tttataccag tgccccttca aaactaaagg gagatcttaa
     2761 ttatctgcga cttgagtggg ggccagattt tcaacagcat gaggcaggtt tgatcggtgc
     2821 tgacgaagtg cccattctga caaccagttc tgctgaactg gcacagcaac agattgcgat
     2881 gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac
     2941 cgttgtcgat agcacaacac tttcacggcc gctttatgcc atatggctgc aaaatagcga
     3001 taaaaatacg ttgattcgcg atcttttgaa aattaacgtg ctggatgaag tgtattaata
     3061 tgaatggctg gcaaggatgc cggtagaagg atttacttcg gagagggtta tttcagataa
     3121 aaaaaatcct tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc
     3181 caacacccgg ttttggagac cggtgctcta ccaattgaac tacgccccta attagggtgg
     3241 cggaacggac gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac
     3301 tgaactaccg ctccaccgaa ttcttttaca accaccggtt ttatgaccgg ctt
//
LOCUS       DAZFKM010000269         3303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFKM010000269 DAZFKM010000000
VERSION     DAZFKM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /locus_tag="ACHKAY_005200"
     CDS             <1..517
                     /locus_tag="ACHKAY_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5703669.1"
                     /translation="YGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRDGAGIWT
                     ICRGATRVDGKPVLPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQKAGIASF
                     CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQ
                     ESALACWGIDR"
     gene            810..941
                     /locus_tag="ACHKAY_005201"
     CDS             810..941
                     /locus_tag="ACHKAY_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703670.1"
                     /translation="MSDKLITPAKVLCVIVGISFSLMLVALFLSLAWVMLSSSGLLG"
     gene            905..1060
                     /locus_tag="ACHKAY_005202"
     CDS             905..1060
                     /locus_tag="ACHKAY_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703671.1"
                     /translation="MGDVVFVGAAGVTVTDDISRALAFAIKWVAVGIAVSPMLYGLAK
                     LVIALKS"
     gene            1072..1158
                     /locus_tag="ACHKAY_005203"
     CDS             1072..1158
                     /locus_tag="ACHKAY_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703672.1"
                     /translation="MSAELIRAMAFAIRIVAIAVLVWAIRWW"
     gene            1380..1565
                     /locus_tag="ACHKAY_005204"
     CDS             1380..1565
                     /locus_tag="ACHKAY_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5703673.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            1966..2292
                     /locus_tag="ACHKAY_005205"
     CDS             1966..2292
                     /locus_tag="ACHKAY_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO5703674.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(2424..2624)
                     /locus_tag="ACHKAY_005206"
     CDS             complement(2424..2624)
                     /locus_tag="ACHKAY_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5703675.1"
                     /translation="MSSKNRPRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2666..3031
                     /locus_tag="ACHKAY_005207"
     CDS             2666..3031
                     /locus_tag="ACHKAY_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5703676.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAASDMPD
                     PTLPEVSGGSGREDDIIGL"
BASE COUNT          775 a          725 c          939 g          864 t
ORIGIN      
        1 atacggcctg tcggctgccg ttctggcgct gattgccgca ggtgcgcctg cgcctgaaat
       61 cctcgaccag tttctggatg aaaaggaagg taaccacacc acggcatacc gtgatggcgc
      121 gggtatctgg accatctgtc gcggtgccac ccgggtggat ggtaagcctg tcctcccggg
      181 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgaacgtg ataaggcgct
      241 ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa ccccagaaag cggggatcgc
      301 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
      361 aattaatgca ggtgatcgca ggggagcgtg tgaggcgatt cgctggtgga ttaaggacgg
      421 tggcagagac tgccgtattc gttcaaacaa ctgctacggt caggtatccc gtcgtgacca
      481 ggagagcgcg ctggcgtgct ggggtatcga cagataagca gaatattttg ctgaaaaata
      541 aggcatggcc acgcgggcgg ataacacgaa atcctgcgaa ctggcgaaac gtaagtgaat
      601 aaaagtaaaa atcccgtttg ttggcagcaa gcggggtttt gtttttatgg cagtaagcta
      661 tgggaggctg ccttgattga ttttagcaaa ctgattaggg agttgcgact catgattagt
      721 caattaccaa actggaaatt tttgctggtc tggagcatcc cttttttatg ggtagtatcc
      781 cagttaattg tggcaattaa ggggtagcta tgtcagacaa actcataacg ccggcaaagg
      841 tcctctgtgt gattgtcggt atttcatttt cactaatgct ggttgctctt tttctgtccc
      901 tcgcctgggt gatgttgtct tcgtcggggc tgctggggtg acagtgactg atgacatcag
      961 cagagcgctg gcttttgcta ttaagtgggt ggctgttggt attgctgtgt ctccgatgct
     1021 atatgggctg gcaaaactgg tcattgcgct gaaatcgtga actttaaaaa gatgagtgct
     1081 gaacttattc gggcaatggc atttgccatt cgtattgtgg ccattgctgt tctggtctgg
     1141 gcaatccgtt ggtggtgaaa tgaaccgcgt tctgtgtgtg gtcatcattg ctctgctggt
     1201 ggcctgtggt gcgcttagtc tggggctgaa tcattaccgc gataacgcca tcacttacaa
     1261 agcgcagcgc gataaaaaag ccagtgaact gaagctggcg aacgcaacca ttactgatat
     1321 gcaggtgcgc cagcgcgatg ttgctgcgct cgatgcaaaa tactcgaggg aattagccga
     1381 tgcgagagct gaaaatgaaa ctctgcgtgc tgatgttgcc gctggtcgta agcgcctgcg
     1441 gatcaacgcc acctgctccg gtaccgtgcg tgaagccacc ggcacctccg gcgtggataa
     1501 tgcaaccggc ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg
     1561 gttgatgaca atgcagaagc agctggaagg ggcgcaggaa tatatccgta ctcagtgcct
     1621 gaaataagtt ttgttgatgc gccgtatcgt cgctgtattc cctcattaac agagaccgca
     1681 gcccgacagg gagactcctc tgcgcgagtg tgcggggata atcaaaaacg atacacaccg
     1741 gggtttaccg cgttaacgga gcgcggcgtt gtcccctcat agtcgccagt ccggtgcgat
     1801 ggtggaagaa acaggacgat gtgttacctc gcaagccctg ttatgtcatg tgtctgattt
     1861 gtgatttaag tcggataatt gtcgttgcca ttaagcagag gattgatgac cgacagggcg
     1921 gcattgttag aataagactt atttttatct gtgcggggaa tgaaaatgaa aagaaatctt
     1981 ccgttaatta ttttgttgtc ttctctggtt atgggctgta cgcaacataa aacagatatg
     2041 ccccgacagt tggttaaggc attaccacaa tatccggcct atgcagcggc aaattatata
     2101 aagggacggg ttgatgtgag gtttgatatt ggtgctgatg gtactgtcac ccgaattgag
     2161 tttattcgtt cagagccgca ccatctgttt gatgagcagg ttgtaaaggc gatggcaaaa
     2221 tggcgatttg agaaggacag gccgtgtaaa ggcgtgaaga aaacgtttat ctttagtcct
     2281 tctgcaccct gattatttca tcagaaatta attatcactc tgttgttatt ctgtacatcc
     2341 cggcagggta agtcttgttc cgtcggatat gaagatgaaa tattgtggga ggacagtggg
     2401 tacctgctcc tgtaaccgaa cgttcatttc tcgttatttg tcatgctggc cgggcgcaga
     2461 tgcgttgcat ctgttgccag ccttctcctg caggcttcaa taacccacgc tgaaaagtta
     2521 ccggaacctt tatgttcaag ggcgatattg atctgttcaa tcatgtgatt ggggaaacgg
     2581 atgttgcggg ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg
     2641 acaagctata tggagaggat tttacatggc tgtgcttcgt acgttaccgg gcagaatcaa
     2701 aacactgaat acccgacggg tgaatattct gaagggtgaa cagcgtcgtg tcagtggcag
     2761 tgcacgtgtt tccctcaagc gtcatatctg gctcagggac gccgggcagt gccgtctctg
     2821 tggtcgtgtg gttgacctct gtgacagtga actcgatcac cgaattgcac ttcagttcgg
     2881 tggtggtaat gaggagacga atctctggac gctctgtacc gaatgccatc gacaaaagtc
     2941 tgctcgtgaa gcggcgagtg atatgccgga cccgacgctg ccggaggtgt ccggaggtag
     3001 tggcagagag gacgacatca tcggactgta acccgacccc ggggggggta tcatccggcg
     3061 taaaaaacga tcgctttgga caccgccccc cgtctcacgc agagaaaaaa ttcccgtttc
     3121 aggccagtta acatgttaac tggctgtccg ggcatttttg cggtttttat ctttattatt
     3181 cagtttgttg cgtggaaaaa atgttaacag gctttttcag caaatgttaa ccaggcagca
     3241 gttaacattt gcggcatgag acgccgggaa aaatgggctg aaccataccc ggctgagtgc
     3301 gtt
//
LOCUS       DAZFKM010000270         3299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFKM010000270 DAZFKM010000000
VERSION     DAZFKM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..173)
                     /locus_tag="ACHKAY_005208"
     CDS             complement(<1..173)
                     /locus_tag="ACHKAY_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703677.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTV"
     gene            complement(163..342)
                     /locus_tag="ACHKAY_005209"
     CDS             complement(163..342)
                     /locus_tag="ACHKAY_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5703678.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(518..1075)
                     /gene="ymfL"
                     /locus_tag="ACHKAY_005210"
     CDS             complement(518..1075)
                     /gene="ymfL"
                     /locus_tag="ACHKAY_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO5703679.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(1119..1319)
                     /gene="ymfT"
                     /locus_tag="ACHKAY_005211"
     CDS             complement(1119..1319)
                     /gene="ymfT"
                     /locus_tag="ACHKAY_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO5703680.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1410..2084
                     /gene="ymfK"
                     /locus_tag="ACHKAY_005212"
     CDS             1410..2084
                     /gene="ymfK"
                     /locus_tag="ACHKAY_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5703681.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYTIKA"
     gene            2753..3115
                     /gene="yfdP"
                     /locus_tag="ACHKAY_005213"
     CDS             2753..3115
                     /gene="yfdP"
                     /locus_tag="ACHKAY_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO5703682.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            3181..>3299
                     /locus_tag="ACHKAY_005214"
     CDS             3181..>3299
                     /locus_tag="ACHKAY_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703683.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPEN"
BASE COUNT          888 a          814 c          761 g          836 t
ORIGIN      
        1 ctgacggtac tcatccccgc gccaatctga cgggcaatgg tttcaattga tggccagcac
       61 acaccttcgt cattactgaa atcagccagg cgggccataa ttgccacgct ggataatttc
      121 atgcctgacg ctgcgcaacc atcccataca tagccggtta atttagtgct catgaccgac
      181 ctctatttcc ctgaatttac gacgaaactg ttcgagcggg ctgaagcact catgctcata
      241 gccttcacgg aggtagataa cccgttgtgt ttccggctcc caacgaatga ctctgacggg
      301 cactccgtag tgatctttga accagcggtt aacttgtcgc aaaggactgt ctccttctgc
      361 cggttgaaat cccccacagc ccactctgca aagctgtggg ttacaatttc cctgtcacct
      421 ggtacattta ctgcatagca atactccacc ttcgcttttc cacccggtac aggaagcgca
      481 atcagttgcg agcgacggta gtgtgttgtt aaactgttca tgcgttagtt tctccacaac
      541 cagaagcaat cgacgccacg acgcccggag ctgcacactc gcgggcgtca ttactttctg
      601 aaacgcaaaa aattttgtag acaagtgctg catgctcctg cagcttcgaa attgagaggt
      661 acagctcgtc gttaattgct gtcttctcat gcggttccac tacaccgtct tcgattgccg
      721 aacgaatctg ttttgaataa ctgccgatct gttcaatgac ttccagtaaa cgctggttaa
      781 tatcggcatt gtccacatcc tcgacgtcag gaagagacac aaagacgcca tttgcagact
      841 gcgccacagc gtcagcaatg aagtgagtgc caccagcacg ttgtaaaatc attgcccatc
      901 ccagcgggaa aatctgatcg ccatcggcac gaaggcggtt aaataatgcg ttctctgtta
      961 catccagcca ctcagcagct tcagcgtaac cccccggcaa cgctgcgata gtttttctga
     1021 cagctttcac gtaccactca ggctgttttt ctactttcca gtgatactta cccacggtta
     1081 gcctcatcgt tctgtggtta aaaattgaag gtgttctgtt aatctttcgg atagatatcc
     1141 ggtcttaagt cagatttcgt aattgcacct gacgtgcatt gctcaagttt tttcgccagc
     1201 acaaaactgg cttttttata accattgaaa accagccgta agtagccagg tgttgagcca
     1261 acttttccgg ccaactcgcc ctgctgttct ttggttaaag agtcccaata cgctttcata
     1321 caacatgtac ctccggtata catattacat gattgaaatg aaccttcaag atacttgtac
     1381 cttatcggta caaaggtttt aatttcgtta tgaaaacaat ccatgacatc cggcggtcta
     1441 acgccagaaa actgagagat ggtgttggcg gaaattcatc ctttgccacc atgattgatc
     1501 gcgagccaac ccaaaccagc aggtttatgg gggatggcgc tactaaaaat atcggtgaca
     1561 gcatggcacg ccacatcgaa aaatgtttcg acctgcctgt cggatggctt gatcaagaac
     1621 accagacaac gaacatcaca aaaaaacctg atgtttcaat cactaacaaa caaataacgt
     1681 tagtccctgt catatcatgg gtacaggccg gagcatggaa agaagttggc tattctgagg
     1741 ttgatttgag cacagcagaa acgtatccct gccctgtacc ctgtggcgaa atgacttata
     1801 tcttgcgggt gattggtgat tcaatgattg atgagtaccg ccctggagac atgatttttg
     1861 ttgatcccga agtccctgcc tgccacggtg acgacgttat tgcattgatg cacgatacag
     1921 gcgaaaccac cttcaagcga ttgatagaag atggaacaca gcgttatctc aaagcattaa
     1981 acccaaactg gcctgagcct tacattaaga ttaacggtaa ttgctctata attggtacag
     2041 tgattttctc gggaaaacca agaagataca caataaaggc ctaatcaata tttatgaacc
     2101 tgcttcggca ggttttttta tacttgacaa tgtacccatg agatacataa tgtatccaaa
     2161 agaaacatga ggcaggcaag attcaaacaa aatttggttg taacacggcg tatggcacat
     2221 gcgtcgttag cggtctgggg acgttaaagg ggacaatcca ctccttgcta gggcaaacaa
     2281 accaggtagc cggaatgtgc aagtcaatga tgatgctgat aagacgccta accagcgtgg
     2341 cgatccggtt tgacgcctgg gaagagacca gggtgcaacg atgagggcat ttatggagcc
     2401 gcgacaaagt gtggtgccgt aactggctaa gtgctctcag cgttgtggtc atccgcgaaa
     2461 tggcgcggcg gtaagtatgg cggggttact ctttccccgt tgaggacacc ggattgtcag
     2521 gttgaccata cgcctgagtg acaaccccac cacaacagcc actgctttgg cggtaccagt
     2581 ttgtacactt gcttccggct ggtaccgctc tttttacaaa acagagaaga gcatcaccgg
     2641 acgacgggct cataacccaa tccacccggg cggctgccac cgcaggtgct cttctctgtt
     2701 ttgtggagaa accaaccgac cttgcagggt cgatatgatg aggagcagca aaatggctag
     2761 cgaacgcagt actgatgtgc aggcatttat cggggagctg gacggcggcg tatttgaaac
     2821 caaaatcggc gctgttctca gtgaagtcgc ttcaggtgtg atgaacacga aaaccaaagg
     2881 gaaggtctcg ctcaacctgg aaatcgaacc gtttgatgag aaccgtgtga aaataaaaca
     2941 caaactctca tatgttcgtc cgactaaccg cgggaaaatt tccgaagaag acaccaccga
     3001 aacgccgatg tatgtcaatc gcggtggtcg cctgactatt ctgcaggaag accagggaca
     3061 attactgact cttgccggtg aacctgacgg aaaactccgc gcagcaggtc attaatatcg
     3121 ttcttaatta actgattatt tatctcatca ctgaatatcc ttatatagtg aggacttatt
     3181 atgtctcaga acttagacgc aaccgcaatt aatcaaatcc atgcccttat ttctgctcag
     3241 ggtgttaatg aaattattag taagattggt gccgatgctg tggcattgcc tgagaattt
//
LOCUS       DAZFKM010000271         3299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFKM010000271 DAZFKM010000000
VERSION     DAZFKM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..305)
                     /locus_tag="ACHKAY_005215"
     CDS             complement(<1..305)
                     /locus_tag="ACHKAY_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703684.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRVEFSA
                     "
     gene            complement(302..2881)
                     /locus_tag="ACHKAY_005216"
     CDS             complement(302..2881)
                     /locus_tag="ACHKAY_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5703685.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKISQQRRDL
                     WKAENQYAVLKEAATKRQLSEQEKSLLTHEKETLEYKRQLAELGDKVEHQKRLNELAQ
                     QAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQS
                     WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASASTGTAIQAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDEIQAQMRDGGVFSG
                     GRR"
     gene            complement(2862..>3299)
                     /locus_tag="ACHKAY_005217"
     CDS             complement(2862..>3299)
                     /locus_tag="ACHKAY_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5703686.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          641 a         1124 c          806 g          728 t
ORIGIN      
        1 gcactgaatt caacccgcag catgcgaacc cgtgatgacc aggcggcaca ggtcaccttt
       61 atctgccgcc aggcataagg cggtgtccac agaaacgcct tccagccacc gtgctctgcc
      121 aggaatgcct ccagatgtcg ggcctcctcc cgggtcacgg aaagcgtcac acggtatgtt
      181 ttcaggtcag cattcagccc cgccgccata cgctgcgaat acccgtcacc aaaacgcact
      241 tcacgcaccg atggctgcga gttcacctcc atatccggct tcactttcca gcgaaatgtt
      301 ttcatcgcct gcctccggaa aagacgccgc catcacgcat ctgcgcctga atctcatcct
      361 gcgccccctt gcgggccatg tcatacaccg ccttcatcag ctgcggcccc gcctgtccgt
      421 tggtgccgtc gttctgaatc accacgtgat tgttctgatt aaaactaatg ccttccgccc
      481 gccgcatctg cgccggactt ccggcaccgc ccacataacc accttccgca tacccgcgca
      541 tcagacgata caggttgccg acgccaatcc ggctggttgc ctccttcgtg aagacaaact
      601 ccccgcggtg gacaataccg gcaggttcgt atttgccacc ggttcccgta aatcctccgg
      661 tcgcgaaatg gaagttcgcc gccgcagcct gaatggccgt ccccgtggag gcagacgccc
      721 caccaccgaa agcaccaccc atggcgctgc caatactccc gacaatcccc accatggcct
      781 gcttcagaaa aatctctgtc agcatggaga gcacagaacg ggtgaaacca cgccagctct
      841 gttcgctgcc ggtcagcatc gctgccatat tctgtgcaat accgtcaaag gtctgcgtgg
      901 ccacgctttt aacctgcgaa aaactgtccg tcgcactttc tgcccactcg ccccagccgg
      961 acttcagacc ggccatccag cttccacgaa gctgctcctc cgcagaccag gtgttcttca
     1021 gtgcagatgt ggccttcgcc agcgcatccg gattatcacc gtacacgtca cgaaggcgct
     1081 gctcttccga ctcccgctgc acctgacggt cggtgaggcc ccgcgcctgg gcactgattg
     1141 ccgcctgctt cgcgccctgc tgctgctcaa accgcgcagc ctgctgtgcc agctcattca
     1201 gccgtttctg gtgttcaact ttgtctccca gctcagccag ctggcgtttg tactccagcg
     1261 tctctttctc atgggtcagc agggattttt cctgctcaga taactgccgt ttcgtggctg
     1321 cctctttcag gaccgcatac tgattttccg ctttccataa atcgcgacgc tgctggctga
     1381 ttttcccatt cgcaccgctg tgtttttcca gcgtccggag ctcggtttca agcgccagca
     1441 gggcagcatg cgcctggtct tcctgacgct caccggctga cactttgact cctgacgact
     1501 tcggcttttt cagcgtcgat tcataatcct ttttcgccgc cgccatcagc gtgttgtaat
     1561 ccgcctgcag gattttcccg tctttcaggg ccttattcag ttcttcctga cgggcggtat
     1621 atttctccag cggcgtcagc agacgctcat acgccttctg cgcctctccg gtatacttca
     1681 gctgtgatgc gtcccgttcg gcccggtccc tggcggccag ttcaccggct ttttccatat
     1741 ccgactgcag cgtggccgct gccagcccca gacgggcatt ttcccggtca ttccatgcgc
     1801 cctgaaggtt ggcccggaaa gaggaggttt taccgcggcg ctggctccga ctctggtacc
     1861 actgccattt tttatccgcc tcatcaaatg ccttctgcgc actggcgagc atatccgctg
     1921 aggattccgg acgaccgata tccagaatgg catcccacat cgatttgaat gccttccctg
     1981 ttttatccgc ccaggtctcc agtgttccca tgttttcttt caggcgacgg gtctgctcat
     2041 caaagccttt cgtggcgata tcgttcgccg cctgcaatgc cccggcctcg tctccggaac
     2101 gctgcagctg tgcaacatac gcaatctgct ctgccgtcac gttacggaac tggcgcgcca
     2161 tcgccatcag tcccgacgtc gggtcagtgg tcagcttccc gaaggcttca gcgactttat
     2221 ccacctccac accggatgca gacgcaaaac gcgcgacact ctggttgatg gcatcaaact
     2281 gttcaccacc acgcacaccg gcattcacca gggctgccag tgactctctc gcctggttaa
     2341 acgtcagccc tgctgcctgc ccggctcttg agagagtcag catacgatcg gcagtcagtc
     2401 cggactgatt accggaaaga accagggttt tattaaacgc tgaaagcgtg gaatccccct
     2461 ggtaccaggc gtacgccagc gcacctgtcg ccaccgccag cgaggtgacc ccgaccatcg
     2521 gcagggtgat cgcaccggca agccccctga gcatggggat catcccgccg aaggagtcct
     2581 tcacctgacc gccctgttgc agcaggatca gccagggatt ctgaccaccg gcaagctgcg
     2641 tggcgatatc cgtaaactgt gcgggcaggg tgcgcatggc cgctttatac tgtccgacgg
     2701 aaatcccggc tttttgcgca gccagcgcct gacggctcag gccctgttca acagcagtgg
     2761 cggtttttct ggcgtcagta tccagtcctg aaaaatgacg ccttacccgg ctcatctgct
     2821 catcgaaacg gaccgcatcc agactcaggt caataataag atcaccaacc ggctgggaca
     2881 tatctcacac ctcccggaat ccccgctgaa gccatcatta atgcggcatc atccaccatg
     2941 acatccgcca catccgcaga cgataaaata tcgccccctc cgtccccacc gaaccggacg
     3001 cctccggcaa gtcctgccgc tttctgcatc agcattttgt cctcatccag cctctccacc
     3061 tgctcttcct catgccgggg gacaagcaga ctgaaatcag agggatgcat atccggatcg
     3121 caaaaaaaca ggctgagtac agcgtacatc agcccggaaa aatgcatatc cagttgggta
     3181 tcctgaaaat aatgcgtgcg gtaaaaatgt cgccagtcgg catattcggt ggatgtcatc
     3241 ccggcaagca tggcgcgcca gtcaggcctc cccatctctc gcgccagtct gagggcaaa
//
LOCUS       DAZFKM010000272         3295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFKM010000272 DAZFKM010000000
VERSION     DAZFKM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..612
                     /locus_tag="ACHKAY_005218"
     CDS             <1..612
                     /locus_tag="ACHKAY_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFO5703687.1"
                     /translation="APTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTI
                     NQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQI
                     IIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVID
                     MPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            683..1282
                     /locus_tag="ACHKAY_005219"
     CDS             683..1282
                     /locus_tag="ACHKAY_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5703688.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            1347..>3295
                     /locus_tag="ACHKAY_005220"
     CDS             1347..>3295
                     /locus_tag="ACHKAY_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5703689.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSAMAAGNSVKAAKT
                     SETNAKSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGVVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDI
                     GTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRA
                     GNGNPKGITGALWQRTLTGFTNFAWV"
BASE COUNT          871 a          841 c          953 g          630 t
ORIGIN      
        1 gcccccacca ttaccagcgg tggcagtcct ccggtatttt ccctgacatc agacggaaag
       61 ctgaccgcta aaaatgcgga tatcagtggc agtgtgaatg cgaactcagg aacgctcaac
      121 aatgtcacga ttaaccagaa ctgtacgatt aagggcatgc tggaggcgac ccaggtcaga
      181 ggggatttcg ttaaagctgt atcaaaagcc ttcccgaaaa aagtcggtac gtggggtaac
      241 acggaaacac caaacggtac ggttacagtc accatcagcg atgatcataa ctttgaccgc
      301 cagattatta ttccgcccat tatttttaac ggtatagcgt atgacgatcc ggggagcgga
      361 aataacccag gaggcacgcg atacacgggt tatggttttg aagttcgcaa aaacggcgta
      421 ttaatcgcat ccagagaaac taaaggggcc attcccggta gttacagtgc agttattgat
      481 atgcctagtg gtggtggtag cgtcactctg gagtttaaga ttttccagaa aggcaatcag
      541 ggggcaggca atatcaccga ttgtacggtg attgtgacca aaaaagctgc ttccggcatc
      601 agtattcgtt gaaatattta taaccccaat aaagggcgtc aggaatgacg ccttttttat
      661 tgcagaaaag cgagaggtaa ttatgcgtaa agtttgtgca gccattttgt ccgcagccat
      721 ctgtctggcc gtatccggtg cgcctgcatg ggcgtctgag cagcaggcca cactgagcgc
      781 agggtatctt catgcccgta cgaacgctcc cggcagcgat aatctgaacg ggattaacgt
      841 gaaataccgt tatgagttta cggacacgct ggggctgatt acgtcattca gttatgccaa
      901 cgctgaagat gagcaaaaaa cgcattacag cgatacccgc tggcatgaag attccgtgcg
      961 taaccgctgg ttcagcgtga tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc
     1021 gtatgcgatg gcgggtatgg cttacagccg tgtttcgact ttctccggag attatatccg
     1081 cgtaactgac aacaagggga aaacgcacga tgtgctgacc ggaagtgatg acggtcgcca
     1141 cagcaacacg tctctggcgt ggggagctgg cgtgcagttt aacccgaccg aatccgtggc
     1201 cgttgacgtc gcttatgaag gctccggcag cggtgactgg cgtaccaaca gtttcatcgt
     1261 gggtgtcggt tataaattct gattagccag gtaacacagt gttatgacag cccgccggtt
     1321 caggcgggct tttttgtggg gtgaatatgg cagtaaagat ttcaggtgta ctgaaagacg
     1381 gcacaggaaa accggtacag aactgcacaa tccagctgaa agcaaaacgt aacagcacca
     1441 cggtggtggt gaacacgctg gcctcagaaa atccggatga agccgggcgt tacagtatgg
     1501 acgttgagta cggtcagtac agcgttattc tgttggtgga aggattcccg ccgtcacatg
     1561 ccgggaccat caccgtgtat gaagattccc gacccggtac gctgaatgat tttctcggtg
     1621 ccatgacgga ggatgatgcc cgtccggagg cactgcgccg ttttgagctg atggtggaag
     1681 aggtggcgcg taacgcgtcc gcggtggcac agaacacggc agccgcgaag aagtcagccg
     1741 gcgatgccgg cacatcagcc cgtgaggcgg caacccatgc gactgatgct gcaggctcag
     1801 cacgtgcagc cagcacatca gccgggcagg ccgcgacgtc ggctcagtca gcgtcttcca
     1861 gcgcaggaac agcatcaaca aaggccacag aagcatcaaa aagtgctgcc gctgcagagt
     1921 cctcaaaaag cgcggcggcc accagtgccg gtgccgcgaa aacgtcagaa acgaatgctg
     1981 cagcgtcaca aaaatcagca gccacttctg catccactgc gacgacgaaa gcgtcagaag
     2041 ccgccacttc agccagagat gcggcggcct ccaaagaggc ggcaaaatca tcagaaacga
     2101 acgcctccac aagcgccagc agtgcagcct cctcggcaat ggcggcagga aattccgtga
     2161 aggcggcaaa aacgtccgag acgaatgcta agtcttctga aacggcagcg gaacagagcg
     2221 cctcagctgc ggcagactct gagacagcag ctgcatcatc ggcccgtgag gcgtccatta
     2281 aggcagagga ggcagcagtc agtgccaccg ccgccggaaa atcggcagaa agcgccgcat
     2341 catccgcttc aacagccaca acgaaggctg gcgaagccac tgaacaggcc agcgcagcag
     2401 cgagttctgc ttccgcagcg aagacatccg aaacgaacgc gaaagcgtcg gaaaccagcg
     2461 cagaatcctc aaaaacggct gccgcatcgt ccgccagttc ggcggcgtca tcggcatcat
     2521 ctgcgtctgc ttcaaaagat gaggcgacca gacaggcgtc agcagcgagg ggcagcgcca
     2581 cgacggcatc cacgaaggcg acagaggctg ctggcagtgc ggcagcggca gcacagagca
     2641 aaagtacggc agaatccgca gcaacgcgcg ctgagacagc ggcaaaacgt gcagaggata
     2701 ttgcatccgc cgtggcgctt gaggatgcga gcacgacgaa aaagggggta gtacagctca
     2761 gcagtgcgac caacagcact tccgagtcac aggcggcaac gccaaaagcc gttaaggccg
     2821 cgtatgacct ggctaacggg aaatacaccg cacaggacgc tacgacagca caaaaaggaa
     2881 ttgtccagct cagtagtgca accaacagca catctgaaac gcttgctgcg acaccgaaag
     2941 cagtgaaagc agctaatgac aatgcgaatg gtcgggtacc ttctgcccgt aaggtgaatg
     3001 gtaaggcgct ttcagcggat ataacactga cgccgaaaga tattggtacg cttaactcaa
     3061 caacaatgtc attcagcggt ggtgctggtt ggttcaaatt agcaacggta accatgccac
     3121 aggcgagttc tgttgtttca attacgttga ttggtggcgc tggatttaac gtggggtcac
     3181 ctcaacaggc aggtatatct gaacttgttt tacgtgcagg taatggtaat ccgaagggga
     3241 ttactggtgc tttatggcag cgcacattga cagggtttac aaattttgcc tgggt
//
LOCUS       DAZFKM010000273         3254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFKM010000273 DAZFKM010000000
VERSION     DAZFKM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKAY_005221"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKAY_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO5703690.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKAY_005222"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKAY_005222"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO5703691.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHKAY_005223"
     CDS             1252..1521
                     /locus_tag="ACHKAY_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO5703692.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKAY_005224"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKAY_005224"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO5703693.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHKAY_005225"
     CDS             2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHKAY_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO5703694.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          823 a          782 c          793 g          856 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattga tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatggcgatt
     1021 ggcattaacc acgacgtgag agagctgcgt cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcaa gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcgca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaatttcc cgtggttaac cggggaagat tactggctgc gacaaacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttacaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgtccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt cactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtc aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggcgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gtatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaaataattc aatgtaaatt
     3241 acataaggcc cgtg
//
LOCUS       DAZFKM010000274         3253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFKM010000274 DAZFKM010000000
VERSION     DAZFKM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..67)
                     /locus_tag="ACHKAY_005226"
     CDS             complement(<1..67)
                     /locus_tag="ACHKAY_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO5703695.1"
                     /translation="MNIENLKTKAEADISEYITKKI"
     gene            complement(239..469)
                     /gene="ynfN"
                     /locus_tag="ACHKAY_005227"
     CDS             complement(239..469)
                     /gene="ynfN"
                     /locus_tag="ACHKAY_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO5703696.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(474..539)
                     /gene="ynfR"
                     /locus_tag="ACHKAY_005228"
     CDS             complement(474..539)
                     /gene="ynfR"
                     /locus_tag="ACHKAY_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO5703697.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(542..730)
                     /gene="ynfQ"
                     /locus_tag="ACHKAY_005229"
     CDS             complement(542..730)
                     /gene="ynfQ"
                     /locus_tag="ACHKAY_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO5703698.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(741..953)
                     /gene="cspI"
                     /locus_tag="ACHKAY_005230"
     CDS             complement(741..953)
                     /gene="cspI"
                     /locus_tag="ACHKAY_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO5703699.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(1316..1813)
                     /gene="rzpQ"
                     /locus_tag="ACHKAY_005231"
     CDS             complement(1316..1813)
                     /gene="rzpQ"
                     /locus_tag="ACHKAY_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO5703700.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(1810..2343)
                     /gene="rrrQ"
                     /locus_tag="ACHKAY_005232"
     CDS             complement(1810..2343)
                     /gene="rrrQ"
                     /locus_tag="ACHKAY_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5703701.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(2340..2651)
                     /gene="ydfR"
                     /locus_tag="ACHKAY_005233"
     CDS             complement(2340..2651)
                     /gene="ydfR"
                     /locus_tag="ACHKAY_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO5703702.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            complement(2656..2871)
                     /gene="essD"
                     /locus_tag="ACHKAY_005234"
     CDS             complement(2656..2871)
                     /gene="essD"
                     /locus_tag="ACHKAY_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5703703.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            3091..>3253
                     /gene="ynfU"
                     /locus_tag="ACHKAY_005235"
     CDS             3091..>3253
                     /gene="ynfU"
                     /locus_tag="ACHKAY_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO5703704.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKA"
BASE COUNT          955 a          734 c          599 g          965 t
ORIGIN      
        1 taattttttt tgttatatat tcagaaatat ctgcttctgc ttttgttttt aagttttcaa
       61 tattcatcat ttcctctttt agtctgttat gactttccag ttacacagta agtcgattat
      121 atggtgcaaa cgtgtaaaag ataagatgaa acatcgcaat aatcaacata cgatagtcta
      181 aattttacac aaacagacaa agagaatttt cctgaattat caatacaata gcatcaaatc
      241 aactcaagag ccttattgct gcttccagaa tttcttctga agtaacatgt cgatccgcgg
      301 ctacataaat gactttatga tctccggtca gagatggaaa ccctgcggcc attacagtaa
      361 ggtgtgtttt ttcgccattt ggatattcac gcatgatggt gttaactcca gtcatcgctg
      421 gcactaccac tgctggttca gagttaaaaa aaactatgat ttttttcatg atgttaccgt
      481 agtatgtgag tatccatcga atagatacca agcaaaaaag ctcccgaagg agccttcatt
      541 ttcacttttt taaatccaac gacagacggc tggcatttaa gtattgtgaa atattatcaa
      601 atgtaatcat cattgattta caaaagatac attttgcccc gaaaggattc atgtcagaaa
      661 catcaaaaga tgatgttcta tactgggaac catgacaaca cgggcatcta aagtgaatat
      721 ggtttgtaat attgtctacc tcaaagcgcc actacatgaa cagcggcagg acctttaggt
      781 ccgttctcaa taccaaattc aacttcctga ttctcagtta atgttttgaa atcgttgctc
      841 tgaattgctg agaaatggac aaacacatct ttgctgccat ctttcggcgt gatgaaacca
      901 aaaccttttt cagggttaaa ccatttcact aaaccagtca ttttgttaga cataattatt
      961 accttttgaa gaaattagcc cttgggcaga atggtccgaa aaaaaatatc agagagaaaa
     1021 accaacaagg aaatctcaag aggtacaaat aataaaatta taacaatgac tgcttcagat
     1081 aaatttgtaa caaaccagaa caccattaac gcatgattaa ccacccatag caaggattac
     1141 ttttgtaaag aaaaacacag caatgaaaga atagctttat ttattaataa aacgtgtcat
     1201 tctgattaag accttttatc ttacccttaa gatttcagga attttggctc atggaagagt
     1261 cctttttatt taaattttac attccgcgat gtaaatgttc cgatttaata ttaccctaca
     1321 tttgatgctt tttatctctt aaagattcat agatctgttg acaagtcact cctgcgatgt
     1381 agcgttcgtc agcaatttca gcataaagct gagcttctgc tgcaatatct ccgagcatgt
     1441 tggtgagcat tccttcggcg gttttggttg ttttgcctct gacggcagcg gcaagatctg
     1501 cggtatgctt cgctgcgtca aggcgtatgg catatttttt tgcttcggca cgcaactggt
     1561 taacactatc agacagataa gcagccctgg cagaaatttc agcagatttc tgttgtgcat
     1621 ctttaacagc ctcatcacgg gctatagttc gcccctgttc aattattcga gcagcaaatt
     1681 gagcatttac ctcttgtgat aatgcggcag catcacgttc cgcccatttt ttttgccatc
     1741 ctctgtcgct ccagacattt ccgacgataa atcctgacaa cacgagaaaa atcaccatga
     1801 atatctgatt cactgttcta tcccccagca ggttaatgcg ctctcctggt cacgacgaat
     1861 aacctgaccg taacagttat ttgaacgaat gcggcaatcg cgtccgccat ccttaatcca
     1921 ccagcgaatc gcttcgcatg cacctttacg atcaccagca ttcagccgct tataaaacgt
     1981 cgacgggaaa cacttaccgg ggccaatgtt atagggacaa aatgacgcga tacccgcttt
     2041 ttgtggttcg gtcagtggta ctttaatatt gcgctccacc catgccagcg ccttatcacg
     2101 ctcaatggcg ttgacctggt cgcatttttc cttcgacagt ttcatattgg gaaaaacggt
     2161 ttttccatcc accactgtgg caccccgaca gatggtccat atgccagaac catcgcggta
     2221 tgccattgtg tggttacctt ctttttcgtc cagaaactgg tcaagtatct gaggagcaga
     2281 tgcgccagca ccaatcagcg ccagaacggc agccgacagg ccgtatctga tttttgtgtt
     2341 catatatatt tatgatgagg acgctcgtgc ttattggcag gattttcaat cttaaaggag
     2401 tactgatgct gcagataaga ctcaactttt tctgacaatt tttctgctac ttccaggaag
     2461 acttgccgga cgctccttct ggctgctgcc tcataaaact ccagcgcagc tccttcaaca
     2521 cggtccatgg cgacatccag gtcaaaaatt tcaccgtcaa agcgttcctt gtcctgtaag
     2581 gctacagtta ccgtaacttt attctcaaaa ttacggactc ctttcacaac cagttcatag
     2641 tcttgagtca ttggattact ctcctctcgc agccttacgc ctgtcttctt taatcttgaa
     2701 ataaagattt gtcagatacg tcagcaggcc aaaaaccagg ctacccagca caccgattgc
     2761 agcccactgt gacggagtta ctttatcgag taactgtaat gcccagaaac cagcattacc
     2821 cgccgatgtg ccataggcaa cacctgttgt taacttatcc attgatttca tatcctcacc
     2881 ccgatgtaca cggatggtgc aatatgtttg aaaaagatcg gagtctacgg ggtagttttg
     2941 acagcacacg ttgttctcaa cggcgctaaa gaaacataca cattaaaaat gtgagtaatt
     3001 attttgaaag aaagtcatat ataaaataat aatacgagaa atgttttcat atttagtgta
     3061 ctgtatacgg ccattataca ggaaaagcct atgtcagaac gtaaagactc aaaatcacgc
     3121 cgtaattatc tcgttaaatg ttcctgccca aactgcaccc aagagtcaga acacagtttt
     3181 tcaagagtac aaaaaggtgc ccttttgatc tgccctcatt gcaacaaagt attccagaca
     3241 aatcttaaag ctg
//
LOCUS       DAZFKM010000275         3198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFKM010000275 DAZFKM010000000
VERSION     DAZFKM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..851
                     /locus_tag="ACHKAY_005236"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHKAY_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHKAY_005237"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHKAY_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHKAY_005238"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHKAY_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          782 a          841 c          763 g          812 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc gggcgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgc
//
LOCUS       DAZFKM010000276         3137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFKM010000276 DAZFKM010000000
VERSION     DAZFKM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..115
                     /locus_tag="ACHKAY_005239"
     CDS             <1..115
                     /locus_tag="ACHKAY_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703705.1"
                     /translation="AKNELEPWTPEGNAPNYCWWEVDPVDAANEAMSYHND"
     gene            148..366
                     /locus_tag="ACHKAY_005240"
     CDS             148..366
                     /locus_tag="ACHKAY_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO5703706.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            368..631
                     /locus_tag="ACHKAY_005241"
     CDS             368..631
                     /locus_tag="ACHKAY_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703707.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            642..767
                     /locus_tag="ACHKAY_005242"
                     /pseudo
     CDS             642..767
                     /locus_tag="ACHKAY_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            978..1073
                     /locus_tag="ACHKAY_005243"
                     /pseudo
     CDS             978..1073
                     /locus_tag="ACHKAY_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1323..1511
                     /locus_tag="ACHKAY_005244"
     CDS             1323..1511
                     /locus_tag="ACHKAY_005244"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5703708.1"
                     /translation="MPDTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WKPSHWMPLPEPPQEVRQ"
     gene            1627..1731
                     /locus_tag="ACHKAY_005245"
     CDS             1627..1731
                     /locus_tag="ACHKAY_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703709.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            1978..2133
                     /gene="hokD"
                     /locus_tag="ACHKAY_005246"
     CDS             1978..2133
                     /gene="hokD"
                     /locus_tag="ACHKAY_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO5703710.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2301..2579
                     /locus_tag="ACHKAY_005247"
     CDS             2301..2579
                     /locus_tag="ACHKAY_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703711.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2581..>3137
                     /locus_tag="ACHKAY_005248"
     CDS             2581..>3137
                     /locus_tag="ACHKAY_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5703712.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          829 a          712 c          838 g          758 t
ORIGIN      
        1 ggcaaaaaat gaattagaac cgtggacacc tgaaggaaac gcaccaaatt attgctggtg
       61 ggaggttgat ccggttgatg ctgcaaatga agccatgagt taccacaacg attaatgtcg
      121 ggaggccgcc cgaaagggcg gtaagaaatg actacattat tcagaaaaga atatccgcaa
      181 aaaagtaggg cgacagaatt tttgtttctc attctgttta tcgtattgat gataccgata
      241 tcccctctaa tttttgtctg ggcaatcggg aaaataattg agccagttac tgaattgtat
      301 accgacgttg tatgggcgtc gttcaacaca ctgcacaata aaattaatcc gtataaggaa
      361 aactgatatg gcaactttga caaaaaaaga acgggcatgg ttaaacgaat tacaggaagt
      421 tcttgatcgc tgtccatcac cgaaaaaaat tggcttttac accattggcg ataaaagcat
      481 ttacctgtat gacctacgcc gcatggatga aatcatggag gctcttgata atcgttcgtc
      541 gatggattgg tgtgttgctg ttcatgatat gaatgcaggg tttgatgaaa agattttgtt
      601 cccctcatca gttgaaagca ctgcgggtta aggagtaaca catgaccact attaccaaag
      661 aacgtattga attgttcatt aaaaatccgg ttgaaaacgg gcttacccgt ggtgaacaaa
      721 tggaactggc acggattgcg ctggcatcgc tggaagcaga gccggtggca aagattatag
      781 ctcattaccc attaggggtt gacgtaggca aacaaaaatt cgtacaggcc attagagagc
      841 ttcctgactt tggcggatat ctatttgccg cccctccagc gccgatagtg ccggaagaaa
      901 tgtattggca ggatgcgcca gttgaaggca gcagcaaagc ggctgcatac gctacaggct
      961 ggaacgattg ccgcgaagcc atgcttcagt ccggaaactt tcgggaaaat aaagattcgt
     1021 caaccaataa ttttcggaaa atcccggaag cgtcaaccag ctctccggta actccggctc
     1081 ttctgcctgg tggtttcacc attgaggagg cgaaggaatt acatgaagac ctggtacgca
     1141 gccacataag caaggcctta agtggcgaaa agatgaaaaa gaaagatcgc gatgctgatt
     1201 tgcgctggat tcatggcgtt atagttcagg cagcgtggtt tgtaaaagca tcactggagc
     1261 agaatgcact atcgggcaac tatccggtaa ctccggatag ttggataagc tgtagtgagc
     1321 gaatgccgga taccaaaaca gccgttcttg ttgccgtgga gtttgacagg aaaggtgact
     1381 ggcgaatgaa atgggcgact tacatcccgg gtcatcctga cgctaatgat gggtggataa
     1441 ttcctggtgc gtcgtggaaa ccgtcacact ggatgccgct accagaaccg ccgcaggagg
     1501 tgcgccaatg aactggcctg aagcatttgc aattacaggc gttgctatgg ctatcgcttt
     1561 tttagtatat gttatttgtc ggtgggggta aaaacgttcg ccgggattaa caccaaagga
     1621 gggaatatgt cggatgatat atcactggca atggaaggtg cgctggctgt tgttgctgtt
     1681 gtgggcgttt actgcctggt tgtgtttttg atggatcgac tagggaactg aattcattac
     1741 gatatgggaa ttcccatatc gggtaaaaac ggtttgcggg aaaaggagag ttaagtagaa
     1801 ttgctgcggg tgcttgaggc tatctgtctc aggcatgaac accaaaaggc agatagagaa
     1861 aagccccagt taacattacg cgtcctgcaa gacgcttaac attaatctga ggcccaatct
     1921 atgtctcaca aatgtaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg
     1981 aagcagcaaa aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg
     2041 gcactggtaa cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca gacggaggtc
     2101 gctgtcttca cagcttacga acctgaggag taagagaccc ggcgggggag aaatccctcg
     2161 ccaccgctga tgtgtcaggc atcctcaacg cacccgcact taacccgctt cggcgggttt
     2221 tgttttttct ggtcgttctg gtttacaatc catccgtcag cctgaacaac tggcacctgc
     2281 tgcgccagca gagacaaccg atggcgcacg ataccaaatt atacaattct gatgattctg
     2341 ccgtctttgc cagcaggcgc ggacggtgtt ttcacgcatt caaatctgac tggtaccagc
     2401 atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc aggcgcggat
     2461 gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc gtcaggctcc
     2521 cttactccga acggccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaat
     2581 gtgcgggtat tacttcgacc tgttctggta ccggaactcg ggctggttat cgttaagcca
     2641 ggccgtgaat ccatgcgggt atttcatggc ggcagggtgc tggtggagtc tgaaccgaaa
     2701 agcatgcgta atctgccgtc cggggtcgtt cctgccgttc gccagccgct ggcggaagat
     2761 aaatcattac tgccattttt cagcaatgag cgtgtgattc gtgctgctgg tggcgctggt
     2821 gcactgtctg actggttatt acgtcacgtt aaatcctgcc agtggcctca tggtgattat
     2881 catcacagcg aaactgtcat acatcgttac ggtaccggcg cgatggtgtt gtgctggcac
     2941 tgcgacaacc agctgcgcga ccagacatcc gaatcacttg agcaacttgc tcaacaaaac
     3001 ctgtcagcat ggatgattga cgtcatccgc cacgcaatga atggcataca ggaacgggaa
     3061 ttatcgctgg ctgaattatc ctggtgggca gtctgcaatc aggtggtgga cgcattacct
     3121 gaggcagtat cgcgtcg
//
LOCUS       DAZFKM010000277         3105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFKM010000277 DAZFKM010000000
VERSION     DAZFKM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..475)
                     /locus_tag="ACHKAY_005249"
     CDS             complement(<1..475)
                     /locus_tag="ACHKAY_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO5703713.1"
                     /translation="MRTSLVTREEMIEAIEQHTICISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIYAPSVQES
                     ELVVVQNQPDEIPVLEWQGVRVVTTETLARGYGVDEANIRNNLSRNLDRFEEGKHY"
     gene            complement(472..693)
                     /locus_tag="ACHKAY_005250"
     CDS             complement(472..693)
                     /locus_tag="ACHKAY_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5703714.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(1282..1839)
                     /locus_tag="ACHKAY_005251"
     CDS             complement(1282..1839)
                     /locus_tag="ACHKAY_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5703715.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKEEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLINE"
     gene            2008..2496
                     /locus_tag="ACHKAY_005252"
     CDS             2008..2496
                     /locus_tag="ACHKAY_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5703716.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHRVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSSANNEPPMDFDDDI
                     PF"
     gene            2694..>3105
                     /locus_tag="ACHKAY_005253"
     CDS             2694..>3105
                     /locus_tag="ACHKAY_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFO5703717.1"
                     /translation="MNNMTTKELLTALPKYKSHKTVRASKIKDIEIIALMDVVLLCNI
                     EVVEPEGVKVHVDKMFLQKHRPEIGGYLVAYEDGSLSYSPEKTFEEGFSRTNDFFENG
                     ALLSIEGHNGVTFITARDVTISAGGIATLQEEIDL"
BASE COUNT          864 a          755 c          734 g          752 t
ORIGIN      
        1 agtaatgctt accttcttca aagcggtcaa ggttgcgaga caaattgttg cgaatattgg
       61 cttcatcgac cccataccct ctagcaagag tttcggttgt cacgacacgt actccctgcc
      121 attccagaac gggaatttca tccggctgat tctgaacaac caccagttcc gattcctgaa
      181 ctgaaggtgc ataaattttt tctgatttaa cgttagttgc tttcattctg tgtgcctcct
      241 tgcgtgcttc ggctgcgacg gttgcgtaat tcagatgacc ctgttcgagc aggtattcgc
      301 ggatatcaga caacaggata cggtgaaccg cgttcttgtc ctttctccgg taaagttgtt
      361 tggtgatcat gaagtagttg gcaataacgc caggtatatc cctggtgctg atacagattg
      421 tgtgctgttc aattgcctcg atcatctctt cacgggtgac taatgacgtt ctcatagtcc
      481 ctcctgagca gaagcgttaa cagggaggca ccagtaactg agagaattgc gtgaatcagt
      541 ggaaaaacgg gcagagaaaa tacatggggc gtcaggaagc tgagagcggg cctcatcttc
      601 tgttggtgca ataacgaagt gatagtggcg tttctggcag gagtaaaagc gccagataaa
      661 ttcagggcgt gcgcaaggat tggcattaac catagttacg gcctcacaat caggtttaac
      721 aacctgctac ccgctgtcaa acaggtggca ggacgtgaca gggttgacag actggcgatt
      781 gtgaaaccag caggccgaag cctccccatc acgccccacc ataatttggg cgtaacgcgg
      841 tttaacggac acaaaaatac cgcaatatcg gaaatctgcg gttgtccgca caatcattca
      901 ggctgtcaaa cctggtcgca gaatttgcta cgacggcgga actataagcc tgaacgacga
      961 tgagatcaat agagccgtgt tcgatggtag gttatttgct ctaataaatc aaatttatta
     1021 gagcaataat ggttaaatca tcttcgcctg cgtgccatat atttgcgcac cgcgcggcgt
     1081 ggacaatctg aagcggtttc tttctgctgc atcaatctcg cagccatgct caaaaatgtc
     1141 aggcacagcc gaagcccggc atataatagc ggttccagtg gccacgtctc attgagcaca
     1201 tataccgcca tgaaaatcga gtcgaaaact atcgccgcca gcgataactt cattgtcgaa
     1261 agtcggcgga gctgccggag tttattcatt gatcagcccc gtcaggcaaa gctggcgttc
     1321 tttttcacgg cgaatcttta aacctcgcag gggcacgccg ttactgttca cgaaatcagg
     1381 gagatggtta cacatattca cccattcctc tttctgcgcc cacttgtgga tggacgtttc
     1441 tactcgcatg cctcgcgctt tgctgtagta ggtccgtaag ctattgcatc ccatattgaa
     1501 tgccgcgctt gtcattgcac tgaaggcatt atcgggcatg tctttgcccc gaaagtgctg
     1561 attaatacag cgttcagcga tcaggatatt cttttcccaa tcagcggcga tttgctggtc
     1621 ggtttttcgc acacccggcg ttaccccgtg tgtattaccg atcccgtcag tccatacccc
     1681 cgccgggcac atgtatggat cacgtcggca accttcagcg ttaccaatca gctcaagccc
     1741 cgcctggttg gttcgcacat tgccattacc catcacgatg gtaatcatca ccgcgatagc
     1801 gcaaattgca ccgcctcctg cggctgtttt tcccttcata aagacctcat aagcgaattt
     1861 tttacgctcc aggacaacac ccattcacag ccaataccga ctgactcgat ccctttagaa
     1921 ggcacaggat aatgcaaatc acttgttagc tacgtttcaa agatatacat tattgctcta
     1981 attaatttat tttattaggt aagataagtg gcacaacgcg gtgtaaacaa agtcatcctg
     2041 attggtaccc tggggcaaga cccagagatc aggtatatac caaatggcgg agcggtcgga
     2101 agactcagca tcgcaacgaa tgaatcatgg cgcgacaagc aaacgggcca acagaaagag
     2161 caaacagaat ggcatagagt cgttttgttc gggaaacttg ctgaaattgc gagtgagtat
     2221 ttacgaaaag gttctcaggt ctacatcgaa gggaaactta aaacccgtaa gtggacagat
     2281 gacgccggtg tagaacgtta cacgacggaa attatcgtca gccagggcgg aactatgcaa
     2341 atgatcggcg cccgccgcga cgattcacag tcctcaaatg gctgggggca atcaaaccaa
     2401 cctcaaaacc accagcaata cagcggtggc ggcaaacctc agagcagcgc caataacgaa
     2461 cctccaatgg actttgacga cgatattccg ttttgaatga gtaaaaaacg attgaaagaa
     2521 aagcggtggt ccagacgcca acaaaagcac gaactcgcta aaaaacgcca aagttggcaa
     2581 tggcacgcgc ttttcacgaa aagaacaccc cgagatattg ctttcgctgg tgggaaaaca
     2641 ttcctgaccc acctgaaggc gcaatacacc aggttttaag cagaggaaag actatgaata
     2701 atatgacaac gaaagagctt ttgacggcac ttccaaaata caaaagccat aaaacagttc
     2761 gtgcctcaaa aatcaaagat atcgaaatta tcgccctaat ggatgtggtc cttctttgca
     2821 acatcgaagt cgttgagcca gaaggggtaa aagtccatgt tgataaaatg tttttgcaaa
     2881 aacaccgacc agaaattggc ggatatctgg tcgcttatga ggacggatct ctgtcctatt
     2941 caccagaaaa aacatttgaa gaaggcttta gtcggactaa cgacttcttc gaaaatgggg
     3001 cattgctcag cattgaaggt cacaatgggg tgacattcat tacagccagg gacgtaacta
     3061 tttctgctgg cggtatcgct actctacaag aagaaatcga ccttg
//
LOCUS       DAZFKM010000278         3077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFKM010000278 DAZFKM010000000
VERSION     DAZFKM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..403)
                     /locus_tag="ACHKAY_005254"
     CDS             complement(59..403)
                     /locus_tag="ACHKAY_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703718.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(432..599)
                     /locus_tag="ACHKAY_005255"
     CDS             complement(432..599)
                     /locus_tag="ACHKAY_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703719.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(672..956)
                     /locus_tag="ACHKAY_005256"
     CDS             complement(672..956)
                     /locus_tag="ACHKAY_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO5703720.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(949..1092)
                     /locus_tag="ACHKAY_005257"
     CDS             complement(949..1092)
                     /locus_tag="ACHKAY_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703721.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1102..1251)
                     /locus_tag="ACHKAY_005258"
                     /pseudo
     CDS             complement(1102..1251)
                     /locus_tag="ACHKAY_005258"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
     gene            complement(1728..1865)
                     /locus_tag="ACHKAY_005259"
                     /pseudo
     CDS             complement(1728..1865)
                     /locus_tag="ACHKAY_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1867..2424)
                     /locus_tag="ACHKAY_005260"
     CDS             complement(1867..2424)
                     /locus_tag="ACHKAY_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5703722.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            complement(2421..2978)
                     /locus_tag="ACHKAY_005261"
     CDS             complement(2421..2978)
                     /locus_tag="ACHKAY_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703723.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            complement(2975..>3077)
                     /locus_tag="ACHKAY_005262"
     CDS             complement(2975..>3077)
                     /locus_tag="ACHKAY_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO5703724.1"
                     /translation="KPMPSREELLKRKSFGSVNDNKYLNAMWRSGKK"
BASE COUNT          730 a          782 c          647 g          918 t
ORIGIN      
        1 atggtaggtc tggatatcat tgagccatga acaggcctca tcgagtgtga ggctgtggtt
       61 agtcctttcg taactcgctg attctcctgt aagtctctgg tgctttgttt ccgtgtatct
      121 tcatttcaga cttcaacaga gcaacgaggg aatcccattc gttgaggatg cctttgaatg
      181 ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc ttctcaacaa
      241 gtgcaacgca tcgtcggaag tctgctgcgt catggggagc gccgaagtga tgaccataga
      301 tattcttttt caggccacat gcgattgagg caagagttgc gctactgatg ccaacatcgc
      361 cagtcgattg ccatttcaaa accttcatag acaaatctga catttcttgt ctccaataaa
      421 aaaaacctcc atcaggcggc ttggtgttct ttcagttctt caattcgaat attggttacg
      481 tctgcatgtg ctatctgcgc ccatatcatc cagtggtcat agcagtcatt gatgttctct
      541 gcttcgataa ctctgttgaa tggttctcca ttccattcac ctgtgactcg gaagtgcatt
      601 tatcatctcc ataaaacaaa atccgccgta gcgagttcag ataaaagaaa tccccgcgag
      661 tgcgaggatt gttattcatt gccgatattc acctttatcg cgaacacctt taccggttta
      721 tcaccgaagt gcggatgtgt gattgtcttg atttcatatc cgtcatacgg aacatcaatt
      781 ctgcggctgg aatcgtcgcg cttcggatat ccctttgtga taatcaggcg gtcatactcc
      841 cggaacataa ttcgcttatt ccaatagtca ttacacaggc gatactcttc cgttttctct
      901 cctcgaatca tggcatcgaa gtattcacct ttgacggcaa gttgtaggtt agccacggtt
      961 aacctcctgc ggagtagcct ttacaagcac tggtgcaaat ccatcttcat taaggttatg
     1021 aatatagact tctgttctcc tgcgctcttc aacgtttaat attgtttctg gatcataaac
     1081 ccatactttc attcgactat tccatgaaga aatcgcttca gatctagttc gtttttctgg
     1141 tccttgggca ccacatttgc atgacacata gcgcattttc cctctgatac tgaatgagta
     1201 tccgatgtta agcacagtgg aaccacagaa tgggcagcga tatagattca taaatcatcc
     1261 accttaggcg cagctgagac agcaacattc cagaactcag aaaacatatt gtatgcacca
     1321 gatagattgg aagtagcata ccctccaagc tcacttctaa tctgaacggc tctcatcatt
     1381 tccggggtta actccttcgg caccataacc caaccatccg gagttaccgg agagttgcca
     1441 ccgggaatat tttccggaat attttgttgt gcgttttgtg gttgttctga tttaccctga
     1501 agcatggcgg cgcggcaggc gttccagcca gctgttcgcc caagcgcgta aacttcagat
     1561 ggatcaagat aatcaatgtc atgcccgtcc tcatcgtcgt tctcaggtaa tgcagcaggt
     1621 actaccggta ctggcggagc ggcgtagacc tcaataattc cattatcaat aggccattct
     1681 ccatccttga ggtagtcact tgtgccgtca acttgctgtt ctgcaatgtg gaatgcacct
     1741 attggttttg cttccagcga tgccagagca atccgtgcca gttccatttg ttcgccacta
     1801 gtaagtccgt tatcaagcgg ggatttaatg aacaattcga tacgttcttt agtgatagtg
     1861 ctcatatcac tctcctttga tgcgaatgcc agctgcgctt gctgattctt catatgcgcg
     1921 tttagcagcg ttaaggattg ctgccagtgg cgtatagccg ccatccattc ggattgtgtt
     1981 gtggatgcca gccattgtgt cgcgcaattt gctgtggcta gcagacagtt ctgctatgca
     2041 cttctctgcg gcatcggctc gatctttttc acgctggaac attttgtgag tgcggtctat
     2101 agcttcgatt cgtgccgctg atgtcttctg cacggcttca agctcaacac gcagcttccc
     2161 aaccgtaaga gcaatatcct cgttcttctg gtcgcggcgt ttgatgtatt gctggtttct
     2221 ttcccgttca tccagtagtg ccagcacggt ttctggcccg gtcagaaatt tgaaggcgtt
     2281 gagcgcatca atatccacac cgtaatcctt aagttcctgt tcagttaaca aatcatcatc
     2341 aactggcaac attaacaggc gttccattgc cggaattgca cgctctgccg cctcacgcag
     2401 tgcctgatag tcaatcttgc tcattggatg actcctttac gaagctgttc ggcgatatct
     2461 tcgagaacgc catcagagaa tgaacggtca aaatcgcctt ccggcgcatt ggccataaac
     2521 tcagtagagg taagaatcat ccgggcaata tcagcggcgt tcttcgcagt atcatcaata
     2581 aaaccagctt cccaggcagc cagcattctg ttcgccacaa agtaagcgcc ctccttgcgt
     2641 gcttcagtct tcacttcagc cagaaaagca tcggtggctg gggtttcagt aatatcatct
     2701 tcccattcgc taaactcctc acgacaaaag tcattaaatt ccttctcaga ttgcttaagc
     2761 gaggaatttt cagcagccat cttcgcgcat ttagcctcaa ggttatcaat cgtgattcca
     2821 gcagaacgac actcccgcaa cgccgtttct agttttgatt caagttcacc gaacttacgg
     2881 acaagatatt catcgtttgt ttcgtcaacc tttaaatcac ttgggatgca tttacctttc
     2941 agaaaaccat ccatctcaat tagtgtcatt tgtttcattt cttcccactc cgccacatcg
     3001 cattcagata tttgttgtca ttaacagaac cgaaactctt tctcttaagc aattcctctc
     3061 tcgatggcat tggcttt
//
LOCUS       DAZFKM010000279         3001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFKM010000279 DAZFKM010000000
VERSION     DAZFKM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..550
                     /locus_tag="ACHKAY_005263"
     CDS             <1..550
                     /locus_tag="ACHKAY_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO5703725.1"
                     /translation="ALNDKSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKST
                     NVRRATAFAISVINDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEM
                     LQDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLD
                     TMLYKFDDNEIITSAIDKLKRS"
     gene            578..1536
                     /locus_tag="ACHKAY_005264"
                     /pseudo
     CDS             578..1536
                     /locus_tag="ACHKAY_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1548..2009
                     /gene="yibG"
                     /locus_tag="ACHKAY_005265"
     CDS             1548..2009
                     /gene="yibG"
                     /locus_tag="ACHKAY_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO5703726.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2644..>3001
                     /locus_tag="ACHKAY_005266"
     CDS             2644..>3001
                     /locus_tag="ACHKAY_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO5703727.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAAD"
BASE COUNT          960 a          537 c          671 g          833 t
ORIGIN      
        1 ggcattgaat gataagagcg cttgcgttcg agctacggca atcgagtcaa cggctcagcg
       61 atgcaagaaa aacccaattt attcacctaa aatagtagaa caatctcaaa ttactgcttt
      121 tgataaatcg actaatgtca gacgtgctac agcatttgct atttctgtta tcaatgataa
      181 agcaacaatt ccactattga ttaatctgtt aaaagatcca aatggagatg tcagaaactg
      241 ggccgcattt gcaataaata tcaataaata tgataatagt gatattaggg attgttttgt
      301 ggagatgctt caggataaaa atgaagaagt ccgtattgaa gcaataatcg gactttccta
      361 cagaaaagat aaaagggttt tatctgtttt atgcgatgag ttaaaaaaaa atactgttta
      421 tgatgatatc attgaagctg cgggtgaatt aggtgataaa acgctacttc ctgttttaga
      481 tactatgttg tacaagtttg atgacaatga aattataact tccgctattg ataagctgaa
      541 gcgttcatag cgtgagttgc ctatgcacag tgggggattc ccgccggcac ggtgcagatg
      601 ctcgaccggc tggaaagtga aatcctggct gaccgggtga gtgaggaaag ccgccgctgg
      661 ctggcatcgt gcggcctgac cgtggagcag atgaaaaacc agatggaccc ggtgtacacg
      721 ccggcgcgaa aaatccacct gtaccactgc gaccatcgcg gcctgccgct ggcgctcatc
      781 agcacggaag gggcaacagc gtggtgcgca gaatatgatg aatggggcaa cctgctgagt
      841 gatgagaacc cgcatcatct gcagcagctc attcgcctgc cgggtcagca gtatgatgag
      901 gagtccggcc tgtattacaa ccgccaccgc tattatgacc cgctgcaggg gcggtatatc
      961 actcaggatc cgattgggct gaagggggga tggaattttt atcagtatcc gttgaatccg
     1021 gtcataaatg tagatccgca aggtttggtt gatataaatt tataccccga aagtgatctt
     1081 atccattctg tagctgatga gattaatatc ccaggcgttt tcacaatcgg ggggcatggt
     1141 acccccacat ctattgaatc cgcaacgcgc agtatcatga cagctaaaga tctagcatat
     1201 ctaattaaat ttgatgggaa ttataaagat gggatgacag tttggttatt ttcttgtaat
     1261 acaggtaaag gacaaaattc atttgctagc caattagcta aagagttaca tacaaatgta
     1321 ataggacctg acacgctatg gacgtggtgg gggcgaggaa ctaatggtaa gttaaaaatg
     1381 gatacagtgc taacagcacc aacgaacctt aattcaaata aggatctaat ggctataaca
     1441 acaaaagacc ttggtaattg gataacatat gggccatctg ggcaccccat ttctaatatg
     1501 caaggtacgc cagaaaaacc cagtgatata agataggttg tagatgtatg aaagcatgct
     1561 tgttactatt tttttatttt tctcttattg gtcaattgca tggtgctgat gtgaaaataa
     1621 aagaaaacga aagtgtgatg ggatctacag caatgaccta tgatttaagt gaagaaaagc
     1681 tgatgaaact gaagtataaa tcacaacatg gtgattcaga ggcatcattt cgactatatc
     1741 aatattactg ctttactaaa aataatattg ataaacaatt gcgattcttg gaaagatcag
     1801 catctcaggg gaatgttaca gcgcagttta attatggggt ctttttatca gatacaaatc
     1861 caacattatc agaatattat aatttgaata gagctattta ttggatggaa ttcgctgtaa
     1921 ataacggtaa tattgatgca aagagcaaac ttcaagagct caaaaagcta aagcgaatgg
     1981 atagaaggaa gaataaggag aatccgtaaa agcaatagtt catcagactg ccgcagcagt
     2041 agtatgatga tgaatcaggg ctgtactata actattatcg gtaacattaa ccgcagcagg
     2101 ggcggtataa cactcaggct ccgattgggc tggaaggtag gtggaatttt tatcagtatc
     2161 cgttgaaccc gatttctgga atcgatcccc ttgggattag ccacctgttt atacagtata
     2221 actttaggta tgctttcgtg tgtttcagat ataccatgtg atgataattt ttatgatgtg
     2281 cttagtattc cagtcgcttc tggtaataat atgcaatgta aaaataatcc ccgttgtaca
     2341 catttataaa tagatggcca atacctcaag gcctatggag ttagaatgtt aatgacccgg
     2401 gagcaagcaa tagaaaacct gacggcatac gattagtacc gtcagttaat acaggaactt
     2461 ataatcgcaa tggattttca atacatagtt gtttaaacgc ttttggtccg tctttaggac
     2521 ctcgattctg ttccgagggt tgtataactg gtttatctaa tgacatgcaa aaattaaatg
     2581 agttaatatt ttcagagcca gatagcacat taacagttac ggactaaaaa atatggcaag
     2641 gttattaaca attgtatttt tttctctatt tctgcgagtg cagttactgc aagacaattt
     2701 cagagagagt tagatttggc agacaaagca atattatggg cactaatatc agccagtact
     2761 aaagagggac gaaaagcgtg ttcactaagc tattttgcct gtaaggccgc agaggctgaa
     2821 ctcggactgg catatatggc tgcgaatgat aacaaggaat ttctcacatc cttatctaat
     2881 ataatgaggt acaaaataga tgctggactt tccgaatcct atacatgcta tttattaagt
     2941 aagggaaaga tcataagacc atatctgaaa aatctaaatc cactccaact tgccgctgat
     3001 t
//
LOCUS       DAZFKM010000280         2972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFKM010000280 DAZFKM010000000
VERSION     DAZFKM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..81
                     /locus_tag="ACHKAY_005267"
     tRNA            6..81
                     /locus_tag="ACHKAY_005267"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHKAY_005268"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHKAY_005268"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(300..1058)
                     /gene="yhdZ"
                     /locus_tag="ACHKAY_005269"
     CDS             complement(300..1058)
                     /gene="yhdZ"
                     /locus_tag="ACHKAY_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO5703728.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1066..2169)
                     /gene="yhdY"
                     /locus_tag="ACHKAY_005270"
     CDS             complement(1066..2169)
                     /gene="yhdY"
                     /locus_tag="ACHKAY_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5703729.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2179..>2972)
                     /locus_tag="ACHKAY_005271"
     CDS             complement(2179..>2972)
                     /locus_tag="ACHKAY_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO5703730.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          808 a          789 c          723 g          652 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcaa aaaggccatc cgaaaggatg gcctttttgc ttttcgaact aacattcaat
      301 taatggatta cctgcgataa aaatgccctc gtacgctctg atttaggatg cgtaaaaaat
      361 tcatcaggtg cagcttgctc cactatttcc ccacgatcca taaaaatcac ccggtcagcg
      421 acggttcgcg caaaccccat ctcatgcgtt acacacaaca ttgtcatacc cgattgcgcc
      481 agcccaatca tcgtatccag cacctctttc accatctccg gatcgagcgc cgacgttggc
      541 tcatcaaaca acataatttt cggcttcata cacagcgaac gcgcaatggc aacgcgttgc
      601 tgctgaccac cagaaatttg tccgggaaac ttatgcgcat gttcggcaat tctcacccgc
      661 tcaaggtaat gcatcgccag agcttcagcc tctttctttg gcatcttgcg tacccaaatc
      721 ggtgccaggg tacagttctg tagaacggtc agatgaggga agagattgaa atgctgaaag
      781 accattccca cttcctgccg gacgcgctca atattgcgga tatcttcatt aagttcgatg
      841 ccatctacca cgatccgtcc ctgttgatgt tcttccagat gattaataca acgaatggtt
      901 gtcgatttac cggaacctga agggccacac agaacgatcc gttctcccgg ttgcacggtt
      961 aaatttatat ttttcaaaac atggaattgt ccataccatt tattgacgtt ttccagcgta
     1021 atcatcgcgt tagcaggttg cagtaaaatt tggctcatag tgtcctcaat gcggtgtacg
     1081 cccggtgtta aaacgttttt ccagatgctg gctatagcgc gacatgctga aacagaagat
     1141 ccagtagatc agcgcggcga aaacataccc ttccgtcgac atacccaacc aggtgggatc
     1201 aacggttgcc tgctgaacgc tactgaaaag atcgaacaac ccgataatga tcaccaggct
     1261 ggtatctttg aagagtgcga tgatggtatt taccagccca ggaattacca gcttcaacgc
     1321 ctgtggcaga ataaccagcc cctgagtttt ccagtaaccc aacgccagcg actctgccgc
     1381 ttcatactgc cctttaggca gtgcctgtaa tccgcctcgc acgacttccg caacatatgc
     1441 tgactgaaac aggatcacgc caaccagcgc gcggatcagt ttgtcgatac tggtgccttc
     1501 tgccataaac aacggcagca tgaccgaaga cataaacaat acggtaatca gcggtacgcc
     1561 gcgccagaac tcgataaaaa tgaccgataa gatacgcaca atcggcatat gggagcggcg
     1621 acctaacgcc agtaagatcc cccacggtag cgcccccgca atcccaacgg atgcaataat
     1681 taaagttagc gtcagcccgc cccattgccg ggtttcaacc cgctccagac caaaaaaacc
     1741 gccatacatc agccaccaga caatcagtgg gtaaatcacc gcccagaccg caatatagcg
     1801 accgcgatgc gggagtattt tccagaacat tggtgcgatt gacacaaggc caatcagtaa
     1861 tgccaggtta attcgccagc gttggtcgtg tgggtaaagt ccatacataa actgaccaaa
     1921 tcgttcgtgg atgaagaccc aacaggcacc ggcttttgtg cagtcggcac gcgttgagcc
     1981 aacccaatta gcctgcaaaa atgcccagtt cagcaacggt gggatcagtt cccacatcaa
     2041 ccatatgcag ccaatagtca gcaggctatt gctccagctg gagaacagat tttttcgcac
     2101 ccacaccatc gcgcggcttg agttatggct cgctgggcgc gagggctgag acagcaatac
     2161 ttttgtcatc atgactcctt agcgttcaac gatcgcgatg cggcggttat agatattcat
     2221 cagcagcgag atagtcaggc tgataatcag atagaccgac atggtcatgg ctatcgtctc
     2281 gatggcttgc cccgtctggt tcagcacggt gccagcaaac agcgaaacca tatcgggata
     2341 gccaatagcg gcggcaagag aggagttttt gacgatgttg agatactggc tggttaacgg
     2401 cggaataatc actcgcagtg cctggggaat aatgacctgg cgtagcgtaa ccgggttggg
     2461 taatcccagt gaccgagccg cttcatgttg accataaggc actgcctgga tcccagcgcg
     2521 gataatctcg gcgataaatg cagatgtata aaccgaaagt gccagcgtta aggctgccag
     2581 ttcaggaatt aaaaccatcc cgccgcggaa gttaaagcct cgtagggctg ggacatccca
     2641 gtgtagtgct gcgccaaaaa gccattgcgc cagtaaaggc aaaccaatga tcaacactgc
     2701 ggcgatcggc caggtgcggc gcagttgtcc ggtctttatc tggtatgttt tattaaagcg
     2761 gaatagccca acagaaagga ttatagccat aacaacagcc aggataaacg caataaatcc
     2821 gtctcccagc tgcggtgacg gaatataaag cccacgattg ctcaaaaagg ccagatcaaa
     2881 cgcgctcaca gcctggcgtg gtccgggcaa attgcgcaac acggcaaagt accagaagaa
     2941 gatttgcagc agcgggggaa tattacggaa ga
//
LOCUS       DAZFKM010000281         2959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFKM010000281 DAZFKM010000000
VERSION     DAZFKM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(127..203)
                     /locus_tag="ACHKAY_005272"
     tRNA            complement(127..203)
                     /locus_tag="ACHKAY_005272"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Arg,seq:tct)
     gene            complement(301..376)
                     /locus_tag="ACHKAY_005273"
     tRNA            complement(301..376)
                     /locus_tag="ACHKAY_005273"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Ile,seq:cat)
     gene            complement(426..1475)
                     /locus_tag="ACHKAY_005274"
     CDS             complement(426..1475)
                     /locus_tag="ACHKAY_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO5703731.1"
                     /translation="MINTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWEGDDDYLKWLDQCLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMA
                     PLISYFRDARKSLGITSKQIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVA
                     AEKHQRGELEKPHHQLVSTYSGLNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(1626..1823)
                     /locus_tag="ACHKAY_005275"
     CDS             complement(1626..1823)
                     /locus_tag="ACHKAY_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO5703732.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(2055..2597)
                     /locus_tag="ACHKAY_005276"
     CDS             complement(2055..2597)
                     /locus_tag="ACHKAY_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO5703733.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(2606..>2959)
                     /locus_tag="ACHKAY_005277"
     CDS             complement(2606..>2959)
                     /locus_tag="ACHKAY_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5703734.1"
                     /translation="IEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRL
                     KLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQRV
                     PGGRLGVKIYKIESE"
BASE COUNT          727 a          811 c          632 g          789 t
ORIGIN      
        1 acaaacactc aatcaatact taacagaact tttatttttg acaaacatat aatattttca
       61 acaatatcct gagccaggta tattccagta taaggctctg ccggaagaaa tctggaagaa
      121 tgaatatggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
      181 ctgtccggct gagctaacaa cgcataatgc agataatgga ttgccatcgg ggacccgggc
      241 cccacacagc cagtttcgaa agctgacgct ctctgccgat gagctaatgg cggtatgtga
      301 tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct cgctctcacc
      361 actgagctaa agggccggta gcagaataat aatggtgcgt aattaattct gcaatcccat
      421 ccgtttcaaa cgattaaatc ctgaacttcc ctgactgtct gttcaaaacg tcctgtctcc
      481 agctcaacac caatcgcaca acgccccagt gccatcgccg cttttaccgt tgaacctgaa
      541 cccataaaaa aatctgcaac caggtctccc ggacgactgc tcgcgttgat tatctgctgc
      601 agcatttctg ccggtttttc gcacggatgt ttccctggat agtactgcac cggtttatgc
      661 gtccagacat cggtgtacgg aacctgcgcc gtcacaccga aataccgccg caaattttta
      721 tattcactca gcagttccgt atactgccgg ttcagcccac tgtatgtgct gaccagctgg
      781 tggtgtggct tttccagttc cccgcgctga tgtttttctg ccgcaacacg cgcaaacaac
      841 gcctgcaatt tgttgtaatt accctcgttc ggtaactgcc actgactggt accaaaccag
      901 tgcgaagcca tgtttttctt tccggtggct tccgctatct gttttgacgt tattcccagt
      961 gatttacgcg catcacgaaa gtaagaaatc agcggggcca tgacgtgctg ttttagctcg
     1021 cgcccctgtg ccacatagcc atcatctttc gggcgatacg gtccctgata atgttctgca
     1081 aacagaatgc gctctgttgc cggaaaatac gcccgcagac tttccttatt gcacccgttc
     1141 cagcgtccgg acggcttcgc ccagataata tggttcagca cattaaagcg ctcacgcatc
     1201 atgatttcgg tgtcagatgc caggcgatga ccaaagaaca ggtaaagact tccggcaggc
     1261 ttcagtaccc gccagaactg cgccagacac tggtccagcc atttcaggta atcatcgtcg
     1321 ccctcccact ggttatccca gccctcgggc ttcactttaa agtatggcgg gtctgtgact
     1381 atcagatcga cagagttttc cggtaaggtc tggataaatt ccaggcaatc agcgttgatt
     1441 aactcacaac tggatatttt tacagtatta atcatagatc aataagcact tctctgatag
     1501 gctcataccg cttttgcgca aagcagatgg gcctgaggtt tgcttgtgac cccaacgcat
     1561 gagcagatgg ctggcaggtg ccgctaacac ccaccagccg cccattacca caaattaaaa
     1621 agccttcact gcggaaggcg tctgtaacaa ccgaactgat aatctgccag acccgccata
     1681 acaagctgag tcagtattaa ctggcagcgt tcgcgtgaaa ggtaagtatt ctgcgcaatt
     1741 tccccgacgg tcgccggttc ggtgacgctt aattcattaa acaccactct ggcggtttcg
     1801 gtcatatcct gctgttttag catgcttttt tcccttttcc ggttaacgtg acataccaat
     1861 acctcttgtc gaaaaagcca gcaagctgaa agaccagtat tcacaactac cagcgcgttt
     1921 aatgttctgt gccgtttttc agacataaaa aaacccgcat aaagcgggtt ctttcaggtg
     1981 tccatgtctg ctattcgcct cgcggtatag ctttgcgaag cgtagctgga ttgaaacagt
     2041 ttatgcgtaa aaaatcaaga cattttttga gcaaacgatt ctcgcatagg gatgtatagc
     2101 gcatattcag caacagccaa ccaattagca attcgctttt cgcatgtact aaaacaccat
     2161 tccgggtgca cctcattcaa caattcagcc attttgcgtt tactcatccc ccgcccttcg
     2221 tacctttgcc gcaggatatc aatcaatcca ggataacgtg caagcgcttt acttatcacc
     2281 ccatcaatgc gtaacgcctc tgcatcagta cagtgagaca accagctctt ctgtctgcca
     2341 gcgatcatct ctcgcaagaa tgcttccagc tctggtttat caatccctga ctccctgatt
     2401 ctacgcaggg cttcattgat tgcggttttt gtcagttttt tggatgccag caactgattg
     2461 aacatatttc ctggtttgcc accacctata tacgaccaac gcccccacat ccgtaatttc
     2521 ccctggatcc agacggcttc cagcgttttt agacgtaaat gctcgccgct tttgcctgta
     2581 atttccgggt atatcatatt tacgatcact cactctcaat tttgtaaatc ttcacgccca
     2641 gccgtccccc aggaacgcgc tgaccgcgca caatattgat ttcatcaaac tgctcgtcgt
     2701 ctatgagaag tccggcatgc gtcagcgcat ccagtggtgc tttcaggata ttgtccaggt
     2761 cacgacgacg tttatccggt ggctctgcaa taatctttat cgccagcctt ccggacaggt
     2821 ttaatttcag ccgctgctgg cgaacaataa gcgccacatc acggcgataa cgctcaccgg
     2881 cttttgatac aaaatatgtg ctgccacgac gtcgccagta agtgttcacc gttggcgggt
     2941 aaggcaaaac aaattctat
//
LOCUS       DAZFKM010000282         2908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFKM010000282 DAZFKM010000000
VERSION     DAZFKM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..845)
                     /gene="espL2"
                     /locus_tag="ACHKAY_005278"
     CDS             complement(<1..845)
                     /gene="espL2"
                     /locus_tag="ACHKAY_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO5703735.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVYFVENES
                     WGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKENKFVINFYDPNQTATH
                     KRVFFCTNNICDIINLTAYDFLSEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLP
                     DNILQGVVINFAMGAGLREIIKKVYNDTRFTDLTKSQMKILCESKNVNNVPGLLLALQ
                     NGHDNV"
     gene            complement(836..1399)
                     /locus_tag="ACHKAY_005279"
                     /pseudo
     CDS             complement(836..1399)
                     /locus_tag="ACHKAY_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1633..2310)
                     /locus_tag="ACHKAY_005280"
     CDS             complement(1633..2310)
                     /locus_tag="ACHKAY_005280"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO5703736.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            2508..>2908
                     /locus_tag="ACHKAY_005281"
     CDS             2508..>2908
                     /locus_tag="ACHKAY_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFO5703737.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVG"
BASE COUNT          740 a          683 c          570 g          915 t
ORIGIN      
        1 acattatcat gtccattttg tagagctaaa agcaacccag gaacattatt aacatttttt
       61 gattcacaca agattttcat ctgagatttt gtaagatctg taaatcgagt atcgttatat
      121 acctttttta ttatctctct tagtcctgct cccatagcaa aattaataac aacaccttgg
      181 agaatattat caggtaattt ttttataaac acattattgt tgtcattcga ttttgtttta
      241 tccacaaaaa gtgacagtgt gtcttctttt agcccataac atttcagaca ttgctcacta
      301 agaaaatcat atgctgtcag gtttattata tcacaaatgt tattagtgca aaaaaatact
      361 cgtttatgcg tcgcggtctg attgggatca tagaaattaa taacaaactt attttccttg
      421 tcgtttttta ttttaatgcc cagagccatt gcatgtccgg gagaatgaac ttctaatgat
      481 gtgtgagatt tattctcttt ctcaagttga agaaaaaggt tatatataac gcttccccaa
      541 gattcatttt caacaaaata cacatcaccc ttaatattat ttgtcccctt gttttcctga
      601 attacaatag ctttgcttaa taattgaggg gatgaaaatg tttcgtagtc tatctttcct
      661 gaattactac aaaaaaactc actccaatat gatgctatat gtcgacacca tatgatttcc
      721 ccattgcaat cactaacctt tccatttaga tttatactgg catctctctt ttttgataaa
      781 taagggggat tgttttttga aatatattca acataatttg atgccgattt gtttattatt
      841 ggcatatgtt tgtatccttt ctataaattt tccgcctgac agtggaaata atatattcta
      901 ctgatttttg tagtttaaac gatttatgaa gaattttact gttgtacagt accagatact
      961 cagccagttt cagattgaac atattcagct attcaaagag caattcttca ttaaactcaa
     1021 taaattgttc cctcagtgtt cggttaaaat gctcacttat ggcatttatt ttcggcgtat
     1081 agagataagt ccatacgaac ccgacggtgg cttcctgtag cgttttgtcg aattttcccg
     1141 agaactcttt gcccatcatc agtgatgatc tggttgatac caacaaggaa caactgggct
     1201 acacagcaga agaaatgcct gatgatatca cttttaagcg atagtacggc cagagagtag
     1261 ttgctgtgtt cgtcgagcat ggtaatgaaa taacggtaca gctcgccatt ctgagctcaa
     1321 tagtatccat gccactgagc ttacaggtct ttatcgggcg atagtgcttt attcttcggg
     1381 gctttgtgga tgatttttcg aaatgttcat gatagttagg taaagctacc gcatatcatt
     1441 tgcaaaatga cagatagata tacttcaatt tgaatcttaa caattagtat gggaatgctg
     1501 tcaatcctct gtatgcgtcc accacatatt gaaatttttg ttctctaata tattccacgg
     1561 gcgtcaagag tgtgaggcct gtgctgttat acagcaccag acgttgtccc ccatctttga
     1621 cctgtctctt gatcagatct cctgatcaag agacttcatc accaggtaac cctcagccat
     1681 atcctgatgt ctgaaccagc catcccacat aactacccaa ccggggctgc ttgtgcgttt
     1741 gctgtcatgc catcgcccca gtttagccag tttcaaacag gcccatttca gggtcggtgt
     1801 ttgcgctgga agctgctttc cttccagctt aacccacagt agtttccact ctgtcggcgt
     1861 cagtattttc tcacagctgt cattttgtgt ttcttcactg atacttccct gccgcaggcc
     1921 cagcacccgc accgcgataa ccaccatgcg ctcaaggtta tcccgggtct gcattcgcag
     1981 cgattccaca catgtaccac cacttttcca cgctttgtgg tattcctcta tcagccagcg
     2041 ttgctcgtaa tggctgacga tacgtcgcgc atcggcggca ctcgccactt tttctgatgt
     2101 cagcggatac cagcaggctc cgtcctctcc tgctccccgc aacagacata cgtgagcgag
     2161 agcgcctggc cgctgttgtc gggatttttt atgctgactt cgctgtaact gatgagcatc
     2221 cgggcctggc gggctgcccg cccgcctttt tgcatcacat tcagcgtgtg gcttcccgcg
     2281 gctgacttta taatgcaggt aatgccagat atccgcttcc cggtcacaga cagtgattac
     2341 ctgtttctgt atctccccca gccgttcggc catgcgctcc gaagcctgct gccagcggta
     2401 actttctttt tctgtcacga acggtgcaat agtggttctt ccgcaacttg ggtggaagaa
     2461 aatggatgaa actttctggt gaacaaagat ctaagctgtt ttaagtcatg ggcaactcaa
     2521 tgcactctct taagacactt ctacagttac cttgcggatg gcgatgcagt cgacaaatta
     2581 ttagctctga cggtataacc ctccatctcc acggaaaacg caaaaaagca caatgtcctg
     2641 aatgctttaa gcgtagcgac tctgttcata gttgtcgtcg gcgccggata cagcatctac
     2701 cctgctcagg acatacgcta tggcttgtat tttccgtccg ccactggtac tgccgtaacc
     2761 cggcttgttc acgtaaaatt tttgccgggt cgcttgctcc cttcgccggt tcacaccaac
     2821 agtcttcaca ggcgttacaa aatttacaac gtcaactggg attaatcgcc ggaggtgagg
     2881 ccgggaaacg ggcagcaacg gcagtggg
//
LOCUS       DAZFKM010000283         2905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFKM010000283 DAZFKM010000000
VERSION     DAZFKM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..488
                     /gene="agaB"
                     /locus_tag="ACHKAY_005282"
     CDS             12..488
                     /gene="agaB"
                     /locus_tag="ACHKAY_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO5703738.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            527..1330
                     /gene="agaC"
                     /locus_tag="ACHKAY_005283"
     CDS             527..1330
                     /gene="agaC"
                     /locus_tag="ACHKAY_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO5703739.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1320..2111
                     /gene="agaD"
                     /locus_tag="ACHKAY_005284"
     CDS             1320..2111
                     /gene="agaD"
                     /locus_tag="ACHKAY_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO5703740.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2112..2867
                     /gene="agaI"
                     /locus_tag="ACHKAY_005285"
     CDS             2112..2867
                     /gene="agaI"
                     /locus_tag="ACHKAY_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO5703741.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          713 a          681 c          728 g          783 t
ORIGIN      
        1 aggattacac aatgagcagt ccaaatattc tcttaacccg tattgataac cgtctggttc
       61 acggtcaggt tggcgtgacc tggacatcca ccatcggtgc gaatctgctg gtagtcgtgg
      121 atgatgttgt cgctaacgat gatattcaac agaaattaat gggtattacc gcagaaacct
      181 acggctttgg cattcgtttc tttactatcg aaaaaaccat taacgtcatc ggcaaagctg
      241 caccgcatca gaagatcttc ctgatttgcc gtacgccaca aacggtacgt aaattggtag
      301 aaggtggcat tgacctgaaa gatatcaacg tcggcaatat gcatttctcg gaagggaaaa
      361 agcaaatcag cagtaaagtt tatgtcgatg accaggatct cgcggactta cgttttatta
      421 aacaacgtgg cgtgaatgtt ttcattcagg acgtccctgg cgatcaaaaa gaacaaatcc
      481 ctgactaaat ctaaaatcgc cttaatattg gtttgaggta ataaaaatgc atgaaataac
      541 cctacttcag ggattatccc tggcggcgtt agttttcgtt ctggggattg atttttggct
      601 ggaagcctta tttttattcc gcccgataat cgtttgtacc ctaactggcg ctattctcgg
      661 tgatattcag actggcttaa ttaccggtgg tctgacagag ttggctttcg ccggattaac
      721 ccctgcaggt ggtgttcagc cgcccaaccc gattatggcg ggtctgatga ccaccgtcat
      781 tgcatggtct acgggcgttg atgccaaaac agcaattggt cttggcctgc cgtttagttt
      841 gttaatgcag tacgtcattc tgttcttcta ttccgctttc tcattattta tgaccaaagc
      901 cgataaatgc gcgaaagagg cggatacggc agcgttttcc cggcttaact ggacaacgat
      961 gctcatcgtc gcttcagcgt atgcggtgat tgctttcctc tgtacttacc tggcacaagg
     1021 ggcgatgcag gcgctggtga aagcgatgcc cgcctggttg acccacggct ttgaagtggc
     1081 tggcggtatt ctgcctgccg ttggttttgg cttgctgctg cgcgtaatgt tcaaagcgca
     1141 atatatccct tacctgatcg ccggtttcct gtttgtttgc tacatccagg tcagcaacct
     1201 gttgccggtt gccgtactgg gcgcaggctt tgcggtgtat gagtttttca atgcgaaatc
     1261 ccggcagcaa gcgcaaccgc agcccgttgc cagtaaaaat gaagaagagg actacagcaa
     1321 tgggatctga aatcagtaaa aaagatatca cccgtctggg ctttcgttcg tcgctgctgc
     1381 aagcgagctt taactacgaa aggatgcagg cgggcggttt tacctgggcg atgttgccga
     1441 tcctgaaaaa gatttataag gacgacaaac cgggcttaag cgcggcgatg aaagataacc
     1501 tcgaatttat taatacccac ccgaatctgg tcggattcct gatggggtta ttaatttcga
     1561 tggaagaaaa aggagaaaac cgcgacacca ttaaaggcct caaagtggca ctgtttggcc
     1621 caatcgccgg gattggcgat gcgattttct ggtttacttt gttgccgatt atggcgggaa
     1681 tttgctcatc atttgccagc cagggaaacc tgctggggcc gattctattt ttcgccgttt
     1741 acctgcttat ctttttcctg cgcgtcggct ggacccacgt cggttattca gtcggcgtga
     1801 aggcgatcga taaagtgcga gagaactcgc agatgattgc tcgctcggca accatcctcg
     1861 ggatcacggt aatcggcggg ctgatcgctt cgtatgtgca tattaacgtg gtgacatcgt
     1921 ttgccatcga cagtacccac agcgtcgcgc tgcaacagga tttcttcgat aaagtcttcc
     1981 cgaacatttt accgatggcc tacaccctgc tgatgtatta cttcctgcgg gtgaaaaaag
     2041 cgcatccggt gctgttaatc ggcgtgactt ttgtgctctc tattgtttgt tccgcattcg
     2101 gcattttgta aatggaacga ggcactgctt ctggcggtgc ctctttactt aaggaatttc
     2161 atcctgtgca aacccttcag caagttgaaa actatacggc gttaagtgaa cgagccagcg
     2221 aatatttatt ggctgtgatc cgtagcaaac cggatgccgt gatttgcctg gcgaccggag
     2281 ccacgccatt actgacgtat cattatctgg tagaaaaaat ccaccagcag caggttgata
     2341 tcagccagct caccttcgtg aagctcgacg aatgggtgga tctgccatta acgatgcctg
     2401 gcacctgcga aactttccta caacagcata tcgtgcagct gctggggctg cgtgaagacc
     2461 agctcatcag ctttcgctcc gaagagataa atgagacaga gtgcgaacgg gtaacgaacc
     2521 tgattgcgcg caaaggcggt ctggatttat gcgttctagg attggggaaa aacggtcatc
     2581 ttgggctgaa cgaaccggga gaaagcctac aaccggcctg ccatatcagt caacttgatg
     2641 ccagaacaca gcaacatgag atgttaaaaa ccacgggtcg ccccgtgact cgtgggatca
     2701 ccttaggcct gaaggatatt ctcaatgccc gcgaggtttt gttattggtg actggcgaag
     2761 gaaagcagga tgcgacagaa cgttttctca cggctaaagt ctccaccgct atcccggctt
     2821 cgtttttatg gctgcacagt aactttattt gtttaattaa tacctaacca gatgaaaatt
     2881 tgctcaaaat ttttaaatta actta
//
LOCUS       DAZFKM010000284         2889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFKM010000284 DAZFKM010000000
VERSION     DAZFKM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..2841
                     /gene="eae"
                     /locus_tag="ACHKAY_005286"
     CDS             22..2841
                     /gene="eae"
                     /locus_tag="ACHKAY_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO5703742.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSST
                     YDLVTKNQLINVGVNNKNAFSVCVK"
BASE COUNT          847 a          559 c          678 g          805 t
ORIGIN      
        1 cattgtggtg gagcccataa catgattact catggttttt atgcccggac ccggcacaag
       61 cataagctaa aaaaaacatt tattatgctt agtgctggtt taggattgtt tttttatgtt
      121 aaccagaatt catttgcaaa tggtgaaaat tattttaaat tgagttcaga ttcaaaactg
      181 ttaactcaaa atgccgctca ggatcgcctt ttttatacgt taaaaacagg tgaaactgtt
      241 gccaatattt ctaaatcaca gggtatcagt ttatcggtaa tttggtcact gaataaacat
      301 ttatacagtt ccgaaagcga aatgatgaag gctggacctg gtcagcagat cattttgcca
      361 ctcaaaaaac tgtctgttga atatagtgcc ttacctgtct taggttcggc acctgttgtt
      421 gctgcaggtg gtgtcgctgg tcatacgaat aaaatgacta aaatgtcccc ggacgcgact
      481 aaaagcaaca cgaccgatga caaggctcta aattatgcgg cacaacaggc cgcgagcctt
      541 ggtagccagc tccagtcgcg ctcactgaac ggcgattacg cgaaagatac cgctcttggt
      601 atggccagca gccaggcttc gtcacagttg caggcctggt tacaacatta tggaacggca
      661 gaggttaatc tgcagagtgg taataacttt gacggtagtt cactggactt cttattaccg
      721 ttctatgatt ccgaaaacat gctggcattt ggtcaggtcg gtgcgcgtta cattgactcc
      781 cgctttacgg caaatttagg tgctggccag cgttttttcc ttcctgaaaa tatgttgggc
      841 tataacgtct tcattgatca ggatttttct ggtgataata cccgtttagg tattggtggc
      901 gaatactggc gagactattt caaaagtagc gttaacggct atttccgcat gagcggctgg
      961 catgagtcat acaataagaa agactatgat gagcgcccgg caaatggttt tgatatccgc
     1021 tttaatggct atttaccatc atatccggca ttaggcgcca aactgatgta cgaacagtat
     1081 tatggtgata atgttgcttt gtttaattcc gataagttgc agtcgaatcc tggcgcggcg
     1141 accgttggtg taaactacac tccgattcct ctggtgacga tggggatcga ttaccgtcat
     1201 ggtacgggta atgaaaatga tctcctttac tcaatgcagt tccgttatca gtttgataaa
     1261 ccgtggtctc agcaaatcga gccacagtat gttaacgagt taagaacatt atcgggcagc
     1321 cgttacgatc tggttcagcg taataacaat attattctgg agtacaaaaa gcaggatatt
     1381 ctttctctga atattccgca tgatattaat ggtactgaac acagtacgca gaagattcaa
     1441 ttgatcgtta agagcaaata cggtctggat cgtatcgtct gggatgatag cgcattacgc
     1501 agtcagggcg gtcagattca gcatggcgga agccaaagcg cacaagacta ccaggctatt
     1561 ttgcctgctt atgtgcaagg cggcagcaat atttataaag tgaccgctcg cgcctatgac
     1621 cgaaatggta atagttctaa taatgtacag ctcactatta ccgttttacc gaatgggcag
     1681 gttgtggacc aggttggggt aacggacttt acggctgata aaacatcggc taaagcggat
     1741 ggcatagaag ctattaccta taccgcgacg gttaaaaaga atggtgtagc tcaggctaat
     1801 gtccctgtaa catttagtat tgtatccggg actgcaactc ttggggcaaa tagtgccaga
     1861 acggatggta acggtaaggc gaccgtaacg ctgaagtcgg ctacgccagg acaggtcgtc
     1921 gtgtctgcta aaaccgcgga gatgacttcg ccacttaatg ccagcgcggt tatatttgtt
     1981 gatcaaacca aggccagtat tactgagatt aaggctgata aaacaacagc gaaggcagat
     2041 ggttctgatg cgattaccta tactgtcaga gtgatgaagg agggggcacc cgtagtagat
     2101 cagaaagtga ccttttctaa ggattttggg accctgaata agactgaagc aacaaccgat
     2161 cagaatggtt atgctactgt aaaattatca tccaatactc ctggcaaggc cattgttagt
     2221 gcaaaagtga gtggagtagg tacagaagtt aaggctacta ccgttgagtt ttttgccccg
     2281 ttgagtattg atggtgataa agtgaccgta attggtactg gtatcacggg ggctctgcca
     2341 aagaactggt tacagtatgg tcaagttaag ctacaggcaa cagggggcaa tggaaaatac
     2401 acatggaaat ccagtaatac taaaattgct tctgttgata actcgggagt gataacctta
     2461 aatgaaaaag ggagtgccac aattactgta gtatctggtg ataatcagag tgcgacatac
     2521 acaattaatg caccgggtag tattgtaatt gctgtggata aaaatactcg agttacgtat
     2581 tttgatgccg aaaacaaatg taagacaaat agcgcaaatt tagcacagtc aaaagaacta
     2641 ttggccaata tctattcaac atggggtgct gcaaataaat atccttacta ttctggttct
     2701 aaatcattga ctgcttggat taaacaatcc tcttctgaac agtcatcagg tgtatcaagc
     2761 acatatgatt tggttacgaa gaaccagttg atcaatgttg gagtaaacaa taagaatgct
     2821 ttttctgttt gtgtaaaata atttaattac atctcaaatc ccggaaaata aaattcattt
     2881 tccgggatt
//
LOCUS       DAZFKM010000285         2885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFKM010000285 DAZFKM010000000
VERSION     DAZFKM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..415
                     /locus_tag="ACHKAY_005287"
     CDS             <1..415
                     /locus_tag="ACHKAY_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO5703743.1"
                     /translation="NKNDLEALSDTRLNEAKCLLDHGFFHGAYYLCGYAVECALKACI
                     AKSFLQHEFPNKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWSE
                     QFRYDNNISKAMAEQLFDAVGDQNSGVLKWVKAHW"
     gene            394..795
                     /locus_tag="ACHKAY_005288"
     CDS             394..795
                     /locus_tag="ACHKAY_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703744.1"
                     /translation="MGKSTLVIGRELTKDMEFSGQFLLKKLKLQNLTIDAAMWFYYPD
                     LSWRYILVISDFSERGPAEIYRKISEINRNSISKKYKPIPLEAIEAKGDSAFVYKMLK
                     GFARVNDGKVRVSNSMVNGLEIVDCLIYELK"
     gene            919..1020
                     /locus_tag="ACHKAY_005289"
     CDS             919..1020
                     /locus_tag="ACHKAY_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703745.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            1017..1472
                     /gene="ybcN"
                     /locus_tag="ACHKAY_005290"
     CDS             1017..1472
                     /gene="ybcN"
                     /locus_tag="ACHKAY_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO5703746.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            1472..1642
                     /locus_tag="ACHKAY_005291"
     CDS             1472..1642
                     /locus_tag="ACHKAY_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5703747.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1635..1925
                     /locus_tag="ACHKAY_005292"
     CDS             1635..1925
                     /locus_tag="ACHKAY_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5703748.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1922..2284
                     /gene="rusA"
                     /locus_tag="ACHKAY_005293"
     CDS             1922..2284
                     /gene="rusA"
                     /locus_tag="ACHKAY_005293"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO5703749.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            2281..2421
                     /locus_tag="ACHKAY_005294"
     CDS             2281..2421
                     /locus_tag="ACHKAY_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5703750.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            2507..>2885
                     /gene="quuD"
                     /locus_tag="ACHKAY_005295"
     CDS             2507..>2885
                     /gene="quuD"
                     /locus_tag="ACHKAY_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO5703751.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNS"
BASE COUNT          811 a          593 c          704 g          777 t
ORIGIN      
        1 gaataaaaac gaccttgaag cattatctga cactaggctt aatgaggcca aatgcttgct
       61 tgatcatggc ttttttcatg gtgcatatta tctttgtggg tatgcagttg aatgtgcatt
      121 gaaggcctgt attgccaagt catttttaca acatgagttt ccaaacaaaa aagtcgtaaa
      181 tgattcatat actcatgatt tgtcgcaact tctcaaaatt gccaacttac atcaaatttt
      241 gattgctgac gcaaaaaatg atgtttcgtt ggagattaac tggtcggtcg ttaaagactg
      301 gagcgagcaa tttagatacg acaataacat aagtaaagct atggccgaac aattgtttga
      361 tgctgtaggt gaccaaaatt ctggagtttt gaaatgggta aaagcacact ggtaatcggc
      421 agagagttga caaaagatat ggaattctca ggtcaatttt tgttaaaaaa actcaagtta
      481 cagaatttaa ctattgatgc tgcaatgtgg ttttattacc cagatctatc ttggagatat
      541 attttagtta tcagtgactt ctcagaacgt gggccggcag aaatatatag aaaaatcagt
      601 gagataaata gaaatagcat atcaaaaaag tataagccga taccattaga agcaattgag
      661 gctaaggggg attcagcttt tgtttataaa atgttaaaag gatttgctag agtcaacgat
      721 ggtaaagttc gcgtttctaa ttctatggta aatggtttag aaatcgttga ctgtctgatc
      781 tatgagttaa aataagaaat ctcttgctgg gtatcattat tgtttaaatg acttttgatt
      841 ttcaataatc aacttgtcat aattaagtca ccggagtttg aactcctccg gtgacttctg
      901 cgctaaacgg ggacgtttat gcgcacatac aatctaaact atcttctccc gtcacagatg
      961 cagaaatgca cctgcgattt tctttatcca gcgtttgacc tctgcggagg tgaagcgtga
     1021 acctcccaca agacggcatc aaactgcatc gcggtaactt caccgctatc ggtcagcagc
     1081 tccagcctta tttggaagac ggcaagtgct ttcgcatggt gcttaaaccg tggcgcgaga
     1141 gacgcagtct ttcccagaat gcacttagcc acatgtggta cagcgaaatc agtgaatacc
     1201 ttatccgcag ggggaaatcg ttcgctaccg cagcatgggt aaaagatgct ctcaaacaca
     1261 catacctcgg ttatgaaacc aaggacctgg ttgatgtcgt aaccggcgaa atcactacta
     1321 tccagtcgtt acgccatacc tccgatcttg ataccggaga gatgtatgtc ttcctgtgta
     1381 aggttgaagc ctgggcgatg aatattggct gccacctgac tattccgcag agctgcgagt
     1441 tccagctgct gcgcgacaag caggaggcgt aatggctaca ccgcttattc gtgtcatgaa
     1501 cggacacatc tacagagtac caaatcgtcg taagcgtaag cctgagctaa aaccatccga
     1561 aataccaaca ctgctcggat ataccgccag cctggttgat aaaaaatggt tgcgactggc
     1621 agcaaggagg aatcatggct gatttgagaa aagcagcgcg tggtcgcgaa tgccaggtaa
     1681 gaatccctgg cgtatgcaat ggcaaccctg aaacgtctgt actggcacat atccggctgg
     1741 ctggactgtg tggcaccggt attaaaccgc cagaccttat tgccaccatt gcctgttctg
     1801 cctgtcacga cgaaatcgac cgccgcacgc attttgtcga tgctgagtac gcaaaagaat
     1861 gcgcgctgga aggtatggcg agaacgcagg ttatctggct gaaagagggg gtaatcaagg
     1921 cgtgaatacc tacagcatca cattaccctg gcctccgagc aataatcgct attaccgcca
     1981 taatcgcggg cgcacgcacg tcagcgcaga ggggcaggca taccgcgata acgtcgcccg
     2041 aatcattaaa aacgcaatgc tggatatcgg cttggctatg cctgtgaaaa tccgcattga
     2101 gtgccacatg ccggatcgcc gtcgccgtga cctggataat ctgcaaaaag ccgcttttga
     2161 cgcactcact aaagcaggtt tctggctgga tgatgctcag gtcgttgatt accgcgttgt
     2221 gaagatgcct gttaccaaag gtgggaggct ggaactgacc atcaccgaaa tggggaatga
     2281 atgatgtttg agttttatat ggcagaacgt cttcgccacc gctgggggcg tctgcgctta
     2341 tatcgtttcc ccggttctgt tttgaccgat taccgaatac tgaagaatta cgccaaaacc
     2401 ctgacaggag caggagtatg aagtcagaga taacaatcaa ctaatactgt ttcgttgatt
     2461 tttgcttgta attggcgttc tggtctgatt tttgtggagt aagttgatgc gtgatattca
     2521 gatggttctt gagcgttggg gagcgtgggc ggctaataat catgaagatg tgacctggtc
     2581 gtccattgcc gccggtttta agggattaat tacttcaaaa gtaaaatctc gcccgcaatg
     2641 ttgtgacgat gacgcgatga tcatttgcgg gtgcatggcc cgtctgaaaa agaacaacag
     2701 cgatttgcac gatttattag tagattatta tgtagtcggt atgacattca tgtcactggc
     2761 aggtaagcat tgctgctctg atggttatat cgggaaaagg ttacagaagg ctgagggcat
     2821 aattgaaggg atgttaatgg cattagatat ccggttagag atggatatcg ttgttaataa
     2881 ctcta
//
LOCUS       DAZFKM010000286         2842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFKM010000286 DAZFKM010000000
VERSION     DAZFKM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHKAY_005296"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHKAY_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO5703752.1"
                     /translation="ENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGES
                     HLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1418..1775)
                     /locus_tag="ACHKAY_005297"
                     /pseudo
     CDS             complement(1418..1775)
                     /locus_tag="ACHKAY_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1995..2759
                     /locus_tag="ACHKAY_005298"
                     /pseudo
     CDS             1995..2759
                     /locus_tag="ACHKAY_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          690 a          729 c          738 g          685 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcaat gtactgaaac gcgacaaatg gggccttgcc
       61 ggtcaactgc gggagcttaa ggaatctaac cttatcctga attcattgct gcttacccct
      121 ctgggagaat ctcatctggt tactgtgggg ggcgagtatc agagttcgtc catgaaagat
      181 ggggttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggacgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcggggggca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagctc
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaacgcca atatcacagg ttttatgact gacttctcta acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatataaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct atggtcagag gatgtcacgc tgtcgctgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataat aaaggtgcac cgctgagtta taccccagaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcgct gagcgctgta
      901 cagaagaaag tatatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaaggtgac ggatgccctg acgctgaatg cggcagtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagtctgtac agtgccggta agagtacgct ttatgccggt
     1081 gattacttcc agacgggatc atcaacaacg ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataacaa caaaacgcta tcactgacgg tagcatacgt
     1201 tgccactgca actcctggcg gaacggtggc aatgttttag gttaagtgcg tttccggtcc
     1261 gctaatgaga tttcgttacc aacaactaat atcatcaccg gaaatgcacg gattattttt
     1321 taactcatca tttacatact tgtccagaaa tttatactcg gctagctctg aattaagggc
     1381 cgcgtccgca tctccattac cgtcttatat cactggcgct gacaattgtc tcacatatag
     1441 ctgcagtatc ggctccatcc ccgtggctac gcgctgaaat tctagtgaac cgctttcgtg
     1501 gacttgagtg aaccaactgt ttagctgtga tttatttggg gggcagaata cggtagaact
     1561 cgagtgccag ttgctgcgct attttctgtt ccggttctgt ttcacaaatc gggacgcata
     1621 attttcttcc ccctgattat tttccagggc accaccccgc agcttcccag ggacagtgaa
     1681 ggaagtcggg cggcaatagc aaacggcggg atccaacctt tacgccattt ggcaacagta
     1741 tctctgatga tagtttcagt gcctgtaact ccccgttact ttattcgtac ccttgtaatg
     1801 gggtgttagc cagccccggc atgattaatt cccccagtcg tccatgattc ggggggatgt
     1861 caccgggtct ggtggggcgc tggtaaccgc tgataggggc ctggtcagac atcatgtcgg
     1921 gacagtctgt aacgtggatg ccggtacctg ctccccaagg ttatctggtt aacccgtata
     1981 aacggggaga cacaatgccc gaatccagcg attacgaatc aatcctggtc tttattggcg
     2041 ttgatgtagg taaagacacc cattacgccg ttgccgttaa tcgttcaggt aaacgtttgt
     2101 tcgacagagt attacccaac gacgaaaaca aactcagagc gctgatatct gacctgaaac
     2161 agcatggaca gatactgctg gtcgttgacc agcctgctac catcagggcg ttacccgtcg
     2221 ccgttgcccg ctctgaaggc gtccttgtcg gataccttcc tggactggca atgcgacgca
     2281 tcgccgattt gcactccggt gaagccaaaa ccgatgcccg tgatgcggcc atcattgccg
     2341 aagctgcacg tactctgcct catgctcttc gctctctaaa actggccgac gaacaaatcg
     2401 ccgaactctc catgctttgc ggcttcgatg atgatcttgc cgcacagaca acacaggcca
     2461 gcaaccgcat ccgcggcttg ttgacccaga tacatccggc tctggagcgc gttctcggcc
     2521 cgcgactgga tcatcctgca gtacttgatc ttctccagcg atatccctca cccgaaaaac
     2581 tcgctttact gggtgagaaa aaactggcaa cccagctctg caaactcgcg ccccgtctgg
     2641 gaaaacgcct tgctgcagat atcccccagg cactgacaca gcaaaccgtc atcgtccccg
     2701 gtacgaatgc tgctgtcgtt gttctcccac gcctggcact ccagctcatc acgctgcgtt
     2761 agccaagaga cgaagtgact ctttgaaccg ccccgggttt cctggagagt gttttatctg
     2821 tgaactcagg ctgccagatc at
//
LOCUS       DAZFKM010000287         2835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFKM010000287 DAZFKM010000000
VERSION     DAZFKM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKAY_005299"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHKAY_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO5703753.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..2833
                     /gene="safA"
                     /locus_tag="ACHKAY_005300"
     CDS             2636..2833
                     /gene="safA"
                     /locus_tag="ACHKAY_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO5703754.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          752 a          664 c          686 g          733 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     2821 tttgcaagac tgaaa
//
LOCUS       DAZFKM010000288         2834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFKM010000288 DAZFKM010000000
VERSION     DAZFKM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /locus_tag="ACHKAY_005301"
     CDS             <1..450
                     /locus_tag="ACHKAY_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFO5703755.1"
                     /translation="GGTTLDVAKVQGQLAGISQVFCDPHVGVSLIADAVLSVMATNGM
                     RTSHHIANTIIEHRHDEAWLRQHIHNDAHYDSLMAVIREKEETLKQRVIRALAGFSGY
                     GRVMVVGGGAEIAAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            455..766
                     /locus_tag="ACHKAY_005302"
     CDS             455..766
                     /locus_tag="ACHKAY_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5703756.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPASQPVPTTQITSRMAGNIRALFP
                     E"
     gene            830..1162
                     /locus_tag="ACHKAY_005303"
     CDS             830..1162
                     /locus_tag="ACHKAY_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFO5703757.1"
                     /translation="MQHIDREKAQRLIERMEALAKEENVNIQKIAECGQIVLRREKDL
                     KQLMSGETSKTWTSGEETIYCSFCNKSQYEVTKVIAGPSVCICNECVDLCNEIIRREV
                     TDKKGKNA"
     gene            1159..1497
                     /locus_tag="ACHKAY_005304"
     CDS             1159..1497
                     /locus_tag="ACHKAY_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703758.1"
                     /translation="MNRKQKQELKYFLRKEIARLEDAESQSSEIPFGMDINDARMLQA
                     YRIAQAALQAKPSDGLVRAVRFYELVKRENPPAETGAWKDAVDWVLKEACQVVNTGIK
                     GDLAQPASKL"
     gene            complement(1989..>2834)
                     /locus_tag="ACHKAY_005305"
     CDS             complement(1989..>2834)
                     /locus_tag="ACHKAY_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5703759.1"
                     /translation="ARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSALALDWFVFG
                     NAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPGKVCHLMNPD
                     INQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQVNRESMDSLK
                     ETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASRDDVLAAHRV
                     PPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVIRFNPYALLD
                     TQPTS"
BASE COUNT          764 a          713 c          769 g          588 t
ORIGIN      
        1 ggcggaacca cgctggatgt ggcaaaggtc cagggacaac tggcaggtat cagccaggtg
       61 ttttgcgatc cacacgtagg cgtttccctg atagccgatg ccgtactgtc ggtgatggcc
      121 actaacggta tgcgtaccag tcaccatatc gccaatacca ttatcgaaca tcgccacgat
      181 gaagcctggc tgcgccagca catccacaat gacgcgcatt acgacagcct gatggcggtt
      241 attcgtgaaa aggaagaaac actgaaacaa cgagtgatcc gcgcgctggc gggtttttcg
      301 ggttacgggc gggtgatggt tgtcggtggc ggggcggaga ttgcggcacc cgctatccgc
      361 gaagcctgcg gagttaatgc gactttcatc gcggacgggg tgccacagtt tgctctggtt
      421 aatgggctgt acgcaatgga caaggagtaa accaatgacg acaccaacca gacggataag
      481 tttctatctg aagcccgccg ccgtcaagaa cgaaggcgaa gcatgcgcct ggctggacag
      541 ccttacacca gaagcccgca aaagcggcca acgtgtggct tttctggccg ggctggcact
      601 tctgaaaatg aatccggcag aggcttaccg actggctgca tgggctgacg atgaggcgtt
      661 atcagtgaca caaaccagga cagaacgccc cgcgtcacag ccagtaccaa ccacacagat
      721 aaccagtcgg atggccggaa atatcagggc gttatttccc gaataacaca acatcagggc
      781 gcatccgccc tgatgacttt aatccgggaa cataaacaag ggggacacaa tgcaacacat
      841 tgacagagaa aaagcgcagc gactgattga gcggatggaa gcgctggcga aagaagaaaa
      901 tgtcaacatc caaaaaatag ctgaatgtgg ccagatagtt cttcgtcgtg aaaaagacct
      961 caaacaactg atgtctggcg aaaccagcaa aacatggact tcaggcgagg agacgattta
     1021 ttgcagcttc tgcaataaat ctcagtacga agtcacaaag gtgattgccg gaccgtctgt
     1081 ttgcatctgc aatgagtgcg tggatttgtg caatgaaatt atcaggagag aagtgacaga
     1141 caaaaaggga aaaaacgcat gaacagaaaa caaaaacaag agttgaaata ctttttacgt
     1201 aaagaaattg ccaggcttga agatgcagaa tcacaatcat cagaaattcc gttcggaatg
     1261 gatatcaacg acgcccgtat gctccaggca taccgcatag ctcaggctgc actacaggca
     1321 aaaccgtcag atggactggt tagagcagta cgattctatg aactggtaaa gcgtgagaat
     1381 ccgccagccg aaaccggagc atggaaagac gctgttgact gggtactcaa agaggcttgt
     1441 caggttgtaa acactggcat caaaggagac ctagcccagc ctgcatcaaa gctatagaaa
     1501 tgagccaaaa cgccctcttc cagggcgttt atttcaatgc acaatagtgc acaaatttgc
     1561 acaatttttt tgaacgactt tttgcccttc cggcccgcgt ggcagttgga tccgtcaagg
     1621 atccgtgcgt gcacaaaaaa acgcgctttt tctgcgcgca ggtgacgggg gaacagcccg
     1681 cgtttcaggg ggtaaatagc atcccctgaa cgatgtcgca gcaacacaac agaatggctg
     1741 tatttctcac gctgagcgtg aaaaagacgt gagggctttt gatttgatgg ggtggcagat
     1801 aaggccgtca aaatcgcact gaggcggcga gaacatgcag ttaacgcggt gggattgcgt
     1861 aagagtctga ccgtcgatga tggcaataag caggaaagcg tcgtgaaatt atctgactga
     1921 tacaggagct ggagagtcgg ggcataaatt ttttatgccc cggcgaagca gcagacaagc
     1981 gaagcgcgtc aggatgtggg ctgggtgtcc agcagtgcat aagggttaaa gcggatcacc
     2041 tcttcgccaa gccagtcatt gatgtgcttc atggcctcca tgacgggcat cagctcgtta
     2101 attgcgtaaa cccgcgcggc cttctccaca tcaccaaacg cacttttttc acccggcatc
     2161 gcccccatca gttgcggcgg gacacggtgc gcagccagca catcatcacg ggatgccgcc
     2221 ttaacattca tgaactcatc ctttgcggtg atctgctgga acggcaaaat ttgcaccccc
     2281 tctttgcccc cgtttggcgc atgaatgagc acgtttttaa acgcaccacc accacgtgca
     2341 ccctgtaacg tttctttcag ggagtccatg ctttcgcggt ttacctgcgc tgcaccgatg
     2401 tagatgatgc acccggcgtg ggatccgttg tcgtaataca gttttctgaa catgtccgcc
     2461 gaatgagaca ggctggccga gagtaatgcg ccgagatatt ccggcatgcc gtaaatttcc
     2521 tggttaatgt ccggattcat caggtggcac actttgccag ggcgaaactg gaacgcgtcc
     2581 ttgccatcct gcacatacca ccatgattca agatcgcttc cgcgtcgcat gtatttcgcc
     2641 agtgcgtgcc gtaatttaag cggttcgccg agcatattgc ttcgaagctc aaggaatgcg
     2701 ttaccgaaca caaaccagtc cagcgccagc gccgagaaat cctgccggga aagcagcggg
     2761 tgcgggatgt agcaaccgag taatacattg cgcttaaagt aaagcgcaga ctgatgccag
     2821 gacgtttgcc gggc
//
LOCUS       DAZFKM010000289         2791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFKM010000289 DAZFKM010000000
VERSION     DAZFKM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..226)
                     /locus_tag="ACHKAY_005306"
     CDS             complement(<1..226)
                     /locus_tag="ACHKAY_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703760.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQ"
     gene            complement(235..327)
                     /locus_tag="ACHKAY_005307"
                     /pseudo
     CDS             complement(235..327)
                     /locus_tag="ACHKAY_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(360..698)
                     /locus_tag="ACHKAY_005308"
     CDS             complement(360..698)
                     /locus_tag="ACHKAY_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703761.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(701..1006)
                     /locus_tag="ACHKAY_005309"
     CDS             complement(701..1006)
                     /locus_tag="ACHKAY_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO5703762.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1321..1971)
                     /locus_tag="ACHKAY_005310"
     CDS             complement(1321..1971)
                     /locus_tag="ACHKAY_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO5703763.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            2052..2237
                     /locus_tag="ACHKAY_005311"
     CDS             2052..2237
                     /locus_tag="ACHKAY_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5703764.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2347..2643
                     /locus_tag="ACHKAY_005312"
     CDS             2347..2643
                     /locus_tag="ACHKAY_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5703765.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVVAGDSKQIDMQF"
     gene            2676..>2791
                     /locus_tag="ACHKAY_005313"
     CDS             2676..>2791
                     /locus_tag="ACHKAY_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5703766.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEA"
BASE COUNT          733 a          625 c          573 g          860 t
ORIGIN      
        1 tttgtttcca ttcctgtaac cactgatttg gtgctggttt aaaattaaca atccaatgcg
       61 caggaaccaa ccatgcataa tgctctgtct gatgaaaagc tatatattga agtgcgaata
      121 tttttatccc atcttcttca actgtcgcct ggaatctcca gaaaacaggc attccatcat
      181 gttcagtttc tgattcagga aaaggtacgc tccatgattt tgtcatatct cacctcaaat
      241 aagtggtttg ctgcctaatt tcattttctg gcgaccaaca caagtcacac ccatttcact
      301 gcgtggcttg ctgtaataaa ttaggttagt tcagccaata aaaaacccac cgaagtgggc
      361 tatgaccatt ttttatttgg atttcgttgg tgagcgtgat taacaactct gtgcattaca
      421 tcctcatatt tttcatcttc aattttttcg acatcgcgag gaaatggtgt tgctaatgct
      481 ttgtcaactt tgtccattgg gtcttcatta atcttatatt caggaccgtc atctatagca
      541 ttaaatccag gtgttacacc gttttttaat gcatatgcta tcctcttttc ccatctcgct
      601 attctcctcc tgtctcgaga tgtaagacct ctatcagata cttttctgtt ttgtccgcgg
      661 tcaggattaa cataaatagt ctttttcacc ataagcatac tcaataagca ccgtacggta
      721 gtttactgta caattttatt ttttggactg catgtatttt gtttcctaat gggtttgaat
      781 ccttgtaata aatacttcta ttttttcgaa caacttcttc tttcttcttg cagcaaaggc
      841 ttcctagtga tgctgctttg tctgctctga cgcaaccaga gagctttagc gcaatttttc
      901 gcgccagtgc ttcattactg cgtcgctcgg caataagttc tgctctgcga gctttgtagc
      961 ggctttttgc cgtacctttg gattctttcc agacaatggt taccatgatg gtctccttta
     1021 agtggctttg gcgcatgacg cgtcgaggtg cttatcttct cgatcgctgt cttgcagctg
     1081 caattcgcgc catccccaaa accactcaag ttctggtctc aacggttagg ttgagagttc
     1141 gtcgatgtta aagagcctgc caatctgttc cgtttggctt ccagcctcct gctgacggct
     1201 taaaattaag acctcttaat tttttggtca agtgcatttt tgaagaaaac ttaattttat
     1261 gggcgcgaat ttagtttgtc tttgattttt aacgggaaat aaaaaagggg cgaaagcccc
     1321 ttaaggaagg tttgctagct tggcatcaac gacaacgcca atgattttac agttcccatt
     1381 gatttcaatc attgggtatt gtggattgag tggtttcagg aattttctac cggcatcaat
     1441 aactaacttt ttgaatgtcg cctcgttttc tccttcaagt ttggcgacta ccagctttcc
     1501 attacgtggt tcgacttctg ggtcgacgag aataatcatc ccttcaggaa tactcagtcc
     1561 tgccggggca gtcattgaat cacctttaac gtcgagccaa aaagagtctt cagaacaatc
     1621 taccgttgtg tcgtaccagt tatctattgc acgcctatga tatggctcta cagcttccat
     1681 ccaacatcct gcgcttaccc aactaattag aggatacgaa cctcttggat catgcctgct
     1741 gtgataggca atgtttgaaa gactatcctc tcctttcaac aggtaatcag gggagcactg
     1801 caaagccttg gctaaggcca ataggttttc gccattgggc tcagtttcag atcgctccca
     1861 ttgggaaata gcaacattag acacgccaac catcttgcca agggcagcct gcctaatctt
     1921 gagttctttt ctgcgagcgc gaatacgctc acccatcagt tgtgtattca tagttaagac
     1981 atcttaaata aacttgactt aagattcctt tggtggataa tttaagtgtt ctttaatttc
     2041 ggagcgagtc tatgtacaaa aaagatgtta ttgaccactt cggaacccag cgtgctgttg
     2101 ctaaagcact aggcattagc gatgcagcag tctctcagtg gaaagaagtt atcccagaga
     2161 aagacgccta tcgattggaa atcgttacag ctggcgccct gaagtatcaa gaaagtgctt
     2221 accgccaagc ggcataagta aattgctctt taacagttct ggcctttcac ctctaaccgg
     2281 gtgagcaaac atcagcggca aatccactgg gtgtgccgct atatatcaat ataggaaaat
     2341 taacaaatgg cacaagcaag ttacagcaag ctaacacagc gagaaattga tcgcgcagaa
     2401 acagatttac tcatcaacct gtcaacgctt acccagcgcg gtctggcaaa gatgattggc
     2461 tgtcatgaat cgaagataag cagaacggac tggagattta ttgcttcggt cttgtgtgct
     2521 ttcggaatgg catcagacat cagtccgatt agcagggctt ttaagtatgc gcttgatgaa
     2581 atcacaaaga aaaaatcccc ggtggtcgcc ggggactcta agcaaattga tatgcaattc
     2641 tgagggaatt actggatcaa tccacaggag tcattatgac aaatacagca aaaatactca
     2701 acttcggcag aggtaacttt gccgaacagg agcgtaatgt ggcagatctc gatgatggtt
     2761 acgccagact atcaaatatg ctgattgagg c
//
LOCUS       DAZFKM010000290         2786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFKM010000290 DAZFKM010000000
VERSION     DAZFKM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..161)
                     /locus_tag="ACHKAY_005314"
     CDS             complement(<1..161)
                     /locus_tag="ACHKAY_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5703767.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEP"
     gene            complement(169..564)
                     /locus_tag="ACHKAY_005315"
     CDS             complement(169..564)
                     /locus_tag="ACHKAY_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5703768.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(561..1139)
                     /locus_tag="ACHKAY_005316"
     CDS             complement(561..1139)
                     /locus_tag="ACHKAY_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703769.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1151..1504)
                     /locus_tag="ACHKAY_005317"
     CDS             complement(1151..1504)
                     /locus_tag="ACHKAY_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO5703770.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1516..1911)
                     /locus_tag="ACHKAY_005318"
     CDS             complement(1516..1911)
                     /locus_tag="ACHKAY_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO5703771.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1953..>2786)
                     /locus_tag="ACHKAY_005319"
     CDS             complement(1953..>2786)
                     /locus_tag="ACHKAY_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5703772.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQL
                     A"
BASE COUNT          577 a          873 c          699 g          637 t
ORIGIN      
        1 ggttctgccg tcatctcgcc cggcgtcaga tccttcacct tagccagtcg ctgccagtca
       61 tcgtctgaca acgggtttgc ataagcatca cccttgccgt tgtaaaccca cagagtggta
      121 ccggcacctt ttaccggctc aaggggattt ggtgttgcca tatcgtcctc acatctcgta
      181 tgtaatggaa taagtcagat ctgcagaact ccataacgcc atatcgtcat cacgacgata
      241 ctcatagccc tgcgtaacca tcgtggtaat cagtcctgcc agtgccggga tatcgctcat
      301 caccggataa atccgggact ccatccacgc atccagctct gaatccggca cctgagcagg
      361 caggaaaact tcaatatgca gctccgcctg ccaggtatcg ctgtccagcg cttcgcccgt
      421 gtattcagcg ccggtgagat aaacggcaac tgccggaaaa tccgcctcat caaaaacagc
      481 ggggcgacca tcaaaaagcg tcgccccggt gtcatgcttc tccagtgcat ccagtacggc
      541 tgcacggagt tcagtatgtt tcatcgcttt attaccattc tcagttgatg ctgcagcgca
      601 tagcccagct ctttcggaag acgttcacgc cgtatccgtt caatattctg tttaaacgcc
      661 gtggtaagcg gcaccgccat cgggattttc accacatcaa tggggtaacg gtttttcccg
      721 gccacacgct gcatgacatg ccaccgccca tttttcagtt gctgaataaa cgcgccggga
      781 atacgacggt ttcccaccac aagcacgctg ccgccacctt tcagggatga acgctgcccc
      841 tttttacgac gcctgcgtcg ggacaggaca acccgcgcat tccccagctt gattacgggc
      901 aaatcccccc ggttaacctt gattctggcc tgcggatttt taaccgtggc ccttttcagc
      961 ctggcccttt cctttaccag tttccggcgt acctttgtct cacgggcaac ctgtgacgca
     1021 gactgcgata tcgcggatga agcaacgcgg ttaatggcca ttgctgacgc accgggcacc
     1081 gccgttttgc tgatacggct gaggttttca acggcctgct caagaccttt tatggccata
     1141 catccccctt tcagcggcga cggttaacgg caggcggcac gccacgccca agccagagat
     1201 gacagcttcc gccatcatcc ggcgaaatcc ggtctatcca gaagttttcc tcaccgatgg
     1261 tcagcgtgtc tccacgccgc agctgccgca catcatcagt ccggacaaac agggacgggc
     1321 tggagccttc aacgcgcacg ccctgtccgg catagctgat attttcaggg tcatcaaaaa
     1381 caccacgtat tactgcgccg gactgctcac cggatgtcat ggtggctgac gttcccatgt
     1441 acccgcgtat cgtttcatcg gcgcgggcaa tggcagcatc gaacaggtta tcgaaatcag
     1501 ccacagcgcc tcccgttatt gcattctggc caggccacgt tctgtcattt cggctgccac
     1561 accggcagag acacgaaacg ccgttcccgg cagcacaaat gccacaggtt catcccgcgt
     1621 ggcgtgaagt gcatcggtat gcagcgtcac cagtgccaca accgtgacca gagcagccgt
     1681 atcagtcacg gtatccgtct gtgctgatac cacctcattt tcatgtccgg tcagcgcatt
     1741 ttccgggctg acagacgtgt cctgaccggc tgcgtcatcc gtgtcatcaa gctcctcttc
     1801 cagctctgcc acacggagcg ccagttcttc tttcgtcccc gtcaggctga catcacggtt
     1861 cagttgttca cccagcgagc ggagacgggc aatcagttca tctttcgtca tggactcatc
     1921 cacagagaaa caatggcccc gaagggccat gattacgcca gttgtacaga cacgaactca
     1981 tcagggtcag ccagcagcat cagcggtgct gactgaatca tggtgaactc tcgcgccgga
     2041 tcgccggtag tcacccagtt tttcgggtaa cgggcagagg cgttaatgcc ttcgcgctgt
     2101 gcgtccgcat cctgaataca gccataggta cgcagaccgc gtgcctgagt gttccccagc
     2161 accatcgtgt tgtccggcag gaagttcttt ttgacgccgt tttccacgta ctgtccggaa
     2221 tacacgacga tggccacatc gccatacatt cccttataag acaccgcttt gcccaggtct
     2281 tttaccgctg tctccagctc ggaatgagag ccgcgacggg tatccagctt atccctgacg
     2341 gctttgaagg aacggaacag cgcccatcct ttcggatcaa acacgatgat attcaccacg
     2401 ccgctggcgt tcagcgcgta ggcttcgata tcgtcggtcg ggtcatacgt ggacttgtca
     2461 cgcttgctcc actccgtgcc accggactgt gtgatgttgt ttgccgcact gcggcccata
     2521 tccacctcaa ccggatcaaa ggcttcaccg gtcatggtgt atttgccctt aagcacggca
     2581 gaaactgcct gcatctcttc gacctgggca atggccagct cttcgtcacg catgttctgc
     2641 atgatgatgc gacggcggcg gtaagccggg tccgccagat tctgcggatc ttcatccggc
     2701 aggcgacgca gggtcatctg cggattcacc tcatgcttcg gcttgacata tcccggcgta
     2761 aattcagagg tggagccgcc acggga
//
LOCUS       DAZFKM010000291         2784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFKM010000291 DAZFKM010000000
VERSION     DAZFKM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKAY_005320"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHKAY_005320"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO5703773.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKAY_005321"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHKAY_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO5703774.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          714 a          676 c          662 g          732 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt ttcgctaccg
     2701 tgaaagtgct acaaagataa agatttagtt tcaaaaagaa aaaatatgta accaaaagta
     2761 aaaattaagg aactttgtga acac
//
LOCUS       DAZFKM010000292         2774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFKM010000292 DAZFKM010000000
VERSION     DAZFKM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..1515
                     /gene="espK"
                     /locus_tag="ACHKAY_005322"
     CDS             154..1515
                     /gene="espK"
                     /locus_tag="ACHKAY_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO5703775.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(1892..>2774)
                     /gene="espN"
                     /locus_tag="ACHKAY_005323"
     CDS             complement(1892..>2774)
                     /gene="espN"
                     /locus_tag="ACHKAY_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFO5703776.1"
                     /translation="SKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGGGFYRYIESFNP
                     ISSETPNKIVYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDADGTFYKSEGLHG
                     GGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIVPTPEHSIKQIY
                     SKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVR
                     IGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAII
                     DRTFGMKENISMVNASK"
BASE COUNT          908 a          592 c          484 g          790 t
ORIGIN      
        1 caaaagttaa aatcaacagt ctgttgattt tgagatataa ctctcttttc taatattgac
       61 accttggctt tgctggttgc taaaattagc ttgttgatta gcagtatgga tagaacttac
      121 actgttattt acttctaaaa atggaattta tcaatgcttc ctacatcgca attacgaccg
      181 tccgggacat tctactccaa ttccgcagaa acatcagcag gcatcaaaag cgaaatcaca
      241 ccaactcaga tagaagaagc gcgggccatt ggtcgtttat atatcaaaga ttgtgatatt
      301 gagtatctgc cacagttacc aaacgaaata acatcagtta caatcgaaaa ttgcaataac
      361 ctgaccaccc ttacaggatt accggttaat acacaaaacc tgtccgtcat taactgtgaa
      421 aaattacaaa tcacagacat accatcgaca gtaaaaaatc tacatattga attaactgat
      481 tcaccattta tacacatcat atctgaaggc atcgaatgcc tgacggtttg ccactgccat
      541 atatctggag tgccagagag tgtccgtcat cttgagatca gaggttacgc cacagacggc
      601 ataaaaaatg ttccaaacgg gttattatct ctcagcatca atcgctacac cccagaaaat
      661 caggccagaa ttgacagcct gatatcaccg tcactacaga cgctatctct gactggatgt
      721 agcaatatta tactgccgga gaaactcccg gagagtctga gatctgtaac cattcatgtg
      781 gagcagaaaa ctacatggaa catcgtagct gaaggggttc ctgatggact ggatcttgat
      841 ttacaaaatg tactactctc tccgaatgta attaaagaaa aaaacatcac ttttcagggc
      901 aacgcactgg atgctgcctt gcattttcgt ccgggagaca ttgtctatgg gctatcctct
      961 cccagagaaa aacttgtcaa cagcatcaaa ctggttaacg acttttccaa aaaagatatc
     1021 gtaattcaga atacattaac aaacgcagta tgggacgcca gaaccccccg taaatataag
     1081 caagactcgc ttatcaaaag agcattaaat gaacatgaaa gaggaacaaa atttaaacag
     1141 tgcttaaaaa atcacaataa ttataatgtc atcatggccg acctttccat atacaatcga
     1201 gacaagttat gggcaaaaac aagcaaggca ggcctagagt ttcaaacatt aattcgcaac
     1261 agaacggtta tgttttgtgc ggatgggctt gtcaattcac tcaaactcat agctaacaag
     1321 tcagagggct atggtcagag tattaccgcc agcgaattac gatggattta tcgtaataaa
     1381 gacaacagcc aaataatgaa aaacataaaa ttttatctgc atggcaaaga gataccagca
     1441 gaaagaatat tagatacacc agagtggaaa gactaccgtc caaaatactc cggttccaca
     1501 tataaatatt cttaatgata gcaaaaaata tattttcgat ataatcaatg ttatgattaa
     1561 agagtatttc atcagggcag gtaaaaacag agtaaatcag caaaagaagc tgatcttcag
     1621 cgatactgac actaactgac ggtttaagcg gtcgtatgaa gcagcagctt tccgacggac
     1681 tgccatgcgg atcgtttacc ttttgggcta ttccgcccgt catcaagcgg ctcacgagta
     1741 ctgagtttat caggatgata ttgctgacaa tggtaattcg ttgaccgatg tgtacttcac
     1801 tatatatcgg tcaacaccgt actgccgtgc aacatctcaa agatgtagca tcgtttttac
     1861 cagaggaggc aggacataga ttgtaaaaaa actatttcga tgcatttacc attgagatat
     1921 tttccttcat accaaaagtt ctatcaatta tcgcaagtgg ttcaacaggt gcggtcttat
     1981 ctataaccca accactctct gtaacacttc ctcgtgtgat atattttgcc tttactttaa
     2041 caactattag ataatgctca cgactaaacc gatcagcaac gctaagatcc gatgtgtatt
     2101 caggcaatat tcttccaata cgtacctgct gttgtgccat atattcatct ggtgctttta
     2161 catttggatt tgaatcagga agccctccag cagttttata ttcaacgata ttttcagctt
     2221 gaataccggt tgtgccgcga cacaatatga tgtctgaatc ggacagttct atgtgcccct
     2281 ccttaatttt cgaataaatt tgtttaatac tatgttctgg agttggtact atttcagaag
     2341 ataaagcagg caacgtgtct tcaagcaccg gagaatcatc gtatatatcc aacaaatcct
     2401 catcataccc aatatctcct tctcttaact gagaatacgg tttatctatg tatctaataa
     2461 ctcccccacc atgaagacct tctgatttat aaaatgtacc atcagcgtca cgaaagaaat
     2521 aaacattaac ctgtttgtta tttatagaat acctacctga atataattcc gaatttggct
     2581 ccttagttaa atctactgat tcccccttgt agactatttt atttggtgtc tctgaagata
     2641 tggggttaaa ggattctata tatctataaa aaccaccgcc aactttctca taatacccat
     2701 acttagaaga gttaagatct actgcactat atctattccc cccacgatcc tgtttcattt
     2761 ctaatatttt agat
//
LOCUS       DAZFKM010000293         2768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFKM010000293 DAZFKM010000000
VERSION     DAZFKM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /locus_tag="ACHKAY_005324"
     CDS             <1..517
                     /locus_tag="ACHKAY_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5703777.1"
                     /translation="VAAITCISHPKKTTLRDKAMVNYAFDYLSSPGSLPFTTAATELS
                     AIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLG
                     VKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKN
                     AERLLAGLPAH"
     gene            complement(527..1954)
                     /locus_tag="ACHKAY_005325"
     CDS             complement(527..1954)
                     /locus_tag="ACHKAY_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO5703778.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1951..2658)
                     /locus_tag="ACHKAY_005326"
     CDS             complement(1951..2658)
                     /locus_tag="ACHKAY_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO5703779.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
BASE COUNT          792 a          662 c          612 g          702 t
ORIGIN      
        1 agttgcagca attacctgta ttagccaccc caagaaaaca acgttgcgtg ataaagccat
       61 ggtgaattat gcctttgatt atttaagctc accgggcagt ctaccgttta ccacggcggc
      121 cacggagctt tccgcgattc atggtcactc aacgtcgcaa tatcgccttg gagaatttta
      181 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
      241 atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg gggtggatct acctcaaagg
      301 tctgggggtc aaacccgaca cccgtaaagc aattctctgg tataaggaag cagctgaaca
      361 agggtatgct catgctcaat atactttagg tttgatctac agaaatggct caggtattaa
      421 tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa
      481 tgcggaaaga ttacttgccg ggcttcccgc acattaacgt cgactgtcaa ccgaacagat
      541 ggagtatttt ccctatcaaa ccagcaaaga taaaaatata gaaaataatt ttaatgatgc
      601 ccagacagcc tggcccacca tcgtcaacgg gcattggctg cggttgcggc tccggttcaa
      661 cagtttgctt ctcggtaaaa ttcgccgcct gaattaataa tcgacgaata attaatgctt
      721 ccagtggaca acgatcttct gcatcaaggc ggctcacaat cggcattaat gattcttcgg
      781 ttggttggtt cgcctgcact aattgtttgg caactaaata taatggccat aagggatgtt
      841 ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca
      901 gtgaacgccc cagagcagag agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg
      961 tacgggcgct gtcgccgggg atataaagta gcggttttcc ctgatcgtta accagttcac
     1021 gagcttcaat catcatcacc actaacgggt aataatcgac ccgatgctga cggcaccatt
     1081 gcaatattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtca caaagttctg
     1141 ccagcaagct ttcgccttct ccacaataga cgcgttgtgc gtattcataa taacgcgcat
     1201 cttcattatc agattcagct tcccgccacg accgggcgta atccaggagt tcgggaattc
     1261 ccaggcgacc tgcgctatac cagcggagta aatttctgtg cagttttgca tcatcgggga
     1321 taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa
     1381 tccgagcgaa catttggtaa aaatcgatag tgtcattttg cacggcaacg ggcagatgca
     1441 gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt
     1501 cttcttcgtc tatttccccg acgttttcct cttgcaacca ccgcaatctt tctgctaata
     1561 acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac agcgaccagc
     1621 gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg
     1681 cggagggcag aaaacgttcg ctatcagagg caagtaacgc acgaaaggcg agcagaattt
     1741 catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc gactgcgcaa
     1801 tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg
     1861 gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg tcagtcgttt
     1921 cctctatttc gagaatcttc cagcaatttt tcataggtcg ctcatatcct taagcgtttc
     1981 cgggcatgca gaatagtaag ccaatggttg ccaggacaag gtagggtaat acgcatcatc
     2041 actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa
     2101 gaattcgcgt aataactgca gatgatcatc ttcgggagac tgccaatatt gccgaccaaa
     2161 ggtataaccg gcaaagtatt gccgccagtt acgataataa cgtagagccc gtagatgaat
     2221 gcgcgactgg atccatagac tttcttcttc gctcaaccag ttattaagta cgcccattcg
     2281 gcttaaaaaa cccatccgga cataatccca ggctttaatt cctccctcac cacagcattc
     2341 ggcggtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttagtt cagcaagata
     2401 gtcacgccat tcacatggcg aataacgaaa ccagcggtga taaaaccctg ccaaccgagc
     2461 ggcatgaccg ttatcgatca ttcggtgaat tgtgcgcagc aggtcatcac gggcatcaat
     2521 tccccaggag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg gtgatgaatg
     2581 cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt
     2641 ttgcgactcc atgtccatat aatttatcct taaatattca ttaaatgaat attagccgca
     2701 ttgcgaggct cgtttattta tacttttctg acgcgacata atacgcataa aataaggctt
     2761 acgcaaaa
//
LOCUS       DAZFKM010000294         2767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFKM010000294 DAZFKM010000000
VERSION     DAZFKM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..187
                     /locus_tag="ACHKAY_005327"
     CDS             2..187
                     /locus_tag="ACHKAY_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO5703780.1"
                     /translation="MEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDAGQEVE
                     MPEPEKKKREMIQLWGE"
     gene            190..513
                     /locus_tag="ACHKAY_005328"
     CDS             190..513
                     /locus_tag="ACHKAY_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO5703781.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            510..902
                     /locus_tag="ACHKAY_005329"
     CDS             510..902
                     /locus_tag="ACHKAY_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO5703782.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHYQLPHGAYPA"
     gene            899..1306
                     /locus_tag="ACHKAY_005330"
     CDS             899..1306
                     /locus_tag="ACHKAY_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO5703783.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASALKST
                     VDNLNIISTGVQDMQQVLAQLRVENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNTSPLP"
     gene            1444..1911
                     /locus_tag="ACHKAY_005331"
     CDS             1444..1911
                     /locus_tag="ACHKAY_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703784.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            1904..2539
                     /locus_tag="ACHKAY_005332"
     CDS             1904..2539
                     /locus_tag="ACHKAY_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO5703785.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRMFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            2551..>2767
                     /locus_tag="ACHKAY_005333"
     CDS             2551..>2767
                     /locus_tag="ACHKAY_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO5703786.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMII"
BASE COUNT          676 a          753 c          815 g          523 t
ORIGIN      
        1 actggaaggc gacaccgtgg attcgctctg tttccggtac tacggcacga cgcagggcgt
       61 caccgaaaag gtgctggatg ccaaccccgg actctgtcag caggtatttc tggacgccgg
      121 gcaggaagtg gagatgccgg agccggagaa gaagaaacga gaaatgattc agttgtgggg
      181 ggagtagcag tgagcaccat tcaaacaggg atcacagagc aggttattgc gtggctcttt
      241 gaccacctgc caacggtgta tgcagtaggc gcggcggtca gcatttccgc gctgatgagt
      301 ctttatgacg gacgaacact ggttcagacc gtaacgggat cgctggcgtg cggcgttctt
      361 gccatggccg tggccgggtc gttgcgcttc ttcggttttc ctgaagatgc cgtgacgttt
      421 atcggcgcat caatcggttt tatgggcgca gagaaagcac gcgacaaggt tattgcagcc
      481 tttaatcgca gggtgaagga gaaggacgaa tgagcaacac atttaaattc agcagccgga
      541 gcgaaaagaa tttgcagggc gtaaatcctg atctggtgaa agtgacccga cgggcactgg
      601 aaatttcgga agtggatttt ggtatcaccg aagggttgcg cagccgttac cgccagaagc
      661 aacttgtggc cacaggtaag agccagacca tgaacagccg ccaccttacg ggacatgccg
      721 tggatgttgt ggcttatatc ggcagccagg tgtcatggga gtggccgctg tacgaaaaaa
      781 tcgcagcagc attcagacag gccagccggg aactgaatat tccggtggaa tggggcggcg
      841 actggaagac cctgaaagac ggaccgcatt atcagttacc acacggagcc tatccggcat
      901 gaagctctgg cccacgctgg gcgtcgcttt ccttctgatt gccgcatggg gaacatccat
      961 gcgtctgtcg tggtcgctgg gccgggagaa cgccagaaac gaagcgcagg ccagcgccct
     1021 gaaaagcacc gtcgacaacc tgaatatcat cagcaccggg gtacaggata tgcagcaggt
     1081 gctggcgcaa ctccgcgtgg aaaatcaaca gcgaaatcag gacggagagg ccagacgtga
     1141 acagctacgc aacgatattg caaaagatga atgcgcccac gctttgcctg acgctcgttt
     1201 tactgacagg ttgcgcaggc acgcagaacg cgccacggcc agcgccgtca gtccggctta
     1261 taccgcagac gctgaccata ccggtaacac ctcccccctt ccctgacact cccacatggg
     1321 gaaatctcgg tatatggggc gaccgccttc tggatgcact ggaaacctgt aacgcggata
     1381 aacgggccat tgaattactg gaacagcgca gactgcaacg actgaacaac gaggacaaca
     1441 actatgctga aaactgattc cctgcgtgaa gccatgaccc gttcatgccg atggtgtcag
     1501 gccaacccgg aaaaattcac cattttcgtg gagagcggca acattgaaac gacaggagaa
     1561 accccatcgt ttgtttaccg ctatcagatg gtgatgtttg tcatggatta cgccggggag
     1621 ctggacgacc tcacgctgcc gctgctggcg tggttatccg aaaatcagcc acagttgttg
     1681 ctcaaccctg agcgtaatca ggacatcaaa ttttccgccg ttatcaatga cgatgacagc
     1741 gccgatctcc tgtttacgct ccccctgcgg gaacgcgttc gcatcacgcg cagcagtcag
     1801 ggcacaccgc aggcagaaca cctgccggag ccaaaacccc gcctgccctc ttccgaaggc
     1861 gactggtcgc atgtattcca ggatgtgacg tggggtgaaa gcgatggata aggcattcac
     1921 ccgcgtggat gaaacctttg aggccatccg cgacagcctg aatcagcagg ccatcaataa
     1981 catcgccaga aagctggcac aggatttacg ccgcgcccag caggcgcgca tccggtcaca
     2041 gaaagcgcca gacgggaccg catggacacc acgcagacgc cgcgtaaccc ggatacagga
     2101 acgcattcgc tttatctgga ataacgaagc acgcacgctg aaaaactggc atcacgacac
     2161 ggggaaatac gggcgaacca ttaccgggtg ggatgaggat aaaaacaata tccgcatgtt
     2221 ttaccgggat gacatcgacc gttttctgga aatacgcacc cggcgcatca accaggacag
     2281 cacaaagcgc gtccccatgt tcgtaaaact gcgcaccgcc cgctacctga aagcccgtgc
     2341 agatgcttcc ggtgtgacgg tgggttacag cggcgtggcc gcacgtattg cacgcgttca
     2401 tcagtttggt gagcgcgatc aggttgcgcc gggcattttc accgattacc cggtacgtga
     2461 gctgctgggt atcagtcagg cagatgaacg cctgatttat aacacggtgc tgggccggat
     2521 tgcggaggct gtacggtgag cgcagaactc atgcgactgc tgagcaatat catccgcacc
     2581 gggatcatct ctgaagttga cgagaagtcc tggcgcgtgc gcgttcgcag cggcgaactg
     2641 gaaacaggct ggctgcgctg gaacaccacg cgcgcgggag ccttcaatgt gtggctgccg
     2701 ccatcacctg gcgaacaggt ggtaattgcc tgcattggcg gcaacccgga aaccgccatg
     2761 ataattg
//
LOCUS       DAZFKM010000295         2747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFKM010000295 DAZFKM010000000
VERSION     DAZFKM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHKAY_005334"
     CDS             <1..480
                     /locus_tag="ACHKAY_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5703787.1"
                     /translation="LNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGNYVGA"
     gene            480..1133
                     /locus_tag="ACHKAY_005335"
     CDS             480..1133
                     /locus_tag="ACHKAY_005335"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5703788.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1130..1456
                     /locus_tag="ACHKAY_005336"
     CDS             1130..1456
                     /locus_tag="ACHKAY_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO5703789.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1453..1842
                     /locus_tag="ACHKAY_005337"
     CDS             1453..1842
                     /locus_tag="ACHKAY_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5703790.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTVGAAA"
     gene            1862..2671
                     /locus_tag="ACHKAY_005338"
     CDS             1862..2671
                     /locus_tag="ACHKAY_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5703791.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          672 a          669 c          812 g          594 t
ORIGIN      
        1 ttgaatgaag ttcagaaatt cattgaagcc catccgggat gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt cagcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggaaccgga accacaacca gttcgagaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc acgggtgatt ttgtgcctga cccgccaggc tgaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcggtgctt acggaaaagc
      421 agcaagcgcg ctgcatcggg tgaagagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgagtaataa atattgccag gcgctggtgg aactgcggaa caaaccagcc catgaactga
      541 aggaagtggg cgatcagtgg cgcacgccgg acaacatttt ctggggaatt aacaccctgt
      601 ttggcccgtt tgttctggat ctgtttactg acggtgataa cgccaaatgt gccgcgtatt
      661 acacggcgga agataacgcg ctggcgcatg actggtcaga acgtcttgcg gagcttaaag
      721 gtgctgcctt tggtaatccc ccatacagcc gcgccagtca gcatgagggg caatacatca
      781 ccggcatgcg ttacatcatg aaacatgcca gtgccatgcg tgataagggc gggcgctatg
      841 ttttcctgat caaagctgcc accagcgaag tgtggtggcc ggaagatgcg gaccatattg
      901 cttttattcg cgggcgtatt ggttttgaac tgcctgcctg gtttatcccg aaggatgaga
      961 agcaggtgcc gacaggcgct ttcttcgctg gtgctattgc tgttttcgac aagacctgga
     1021 agggaccggc aatcagctac atcgggcgcg atgaacttga ggcatgtggt gaggcctttc
     1081 tggcgcaggt tcgccagcag gcggaaaaac tggtcaggga gatggcggca tgacgacgtt
     1141 aactcaatgc cagcagcagg tgctggatat gctgatttct taccagcaag aacgtggctt
     1201 tccgccaacc aatcaggagg tggcaaccat gctgggatac cgttcggtga atgcagcggt
     1261 ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg gcgtggcccg
     1321 ggggataact cttcataccg cggtgaagga cgacgacagc gaggcggtcg ggattatccg
     1381 cgcactgctt gccggtgagg aaaacgccag gctgcgtgca gcccactggt tacatgagag
     1441 gggcctgaaa gtatgaagct gatcttgcct ttccctccca gcgtgaacac gtactggcga
     1501 caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg gcgcaaattc
     1561 cagagcgcgg cgtgcgcagc aatagttgag cagttacgtc gtctgccgaa accaacgtcg
     1621 gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg cgatctggac
     1681 aactataaca aggcgctgtt tgacgccctg acccatgcgg gtgtgtggga agacgacagt
     1741 caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa ggtcgagatc
     1801 actatcagta agtacgagaa aacggtgggt gcagccgcct gatcaagagg agaaacgaag
     1861 tatgaataat ctgatggtca ttgatggtat tgaagttcgt cgtgatgctt atgggcgtta
     1921 cagcctgaac gatctgcaca gggcagccgg gggagaacaa aaaaaacgcc cgaaatactg
     1981 gctctccaat aagcaaacct gtgaattgat tgaacaactt ttcaccgagg gtggaattcc
     2041 gcctctggaa aagaatcaac cagttagcgt tattaatggc ggaaataacc aggggacgta
     2101 tgtctgcaaa gaactggtgt atgcctatgc aatgtggatc agcccgtcat tccatctgaa
     2161 ggtgatccgt actttcgata tggtaaccag cgcatcggaa aaattatccg ggcaggctgc
     2221 tgacaagatg caggctggcg tgattctgct ggactttatg cgccgggagt taaacctgtc
     2281 taactcttca gtgcttggtg cctgtcagaa actccaggag gctgttggct taccgaatct
     2341 ggcaccgcgc tatgccattg atgctcctgc tgacgcgcct gatggctcaa gccgtcccac
     2401 gctgtcactg agtgcactgc tgaagcagta tggtatccgc ctgacagcta atcaggcata
     2461 tcaccagatg gtgaagctgg ggatcgtcga gcagcgcgaa cgatacagcc gtaccgcgat
     2521 taacaacatc aaaaaattct ggtcgctgac agcgaaaggc tgcatgttcg gcaagaacat
     2581 caccagtccc gcaaatccgc gcgagacgca gccgcatttc ttcgaatccc gattccctga
     2641 gctgttaaag ctgctcgata ccgttcattg aggtgaccgt gagagcacta ctgacccctg
     2701 aaattgcccc gcgtatgggg atcgtattgt tcaggccagg ttcagag
//
LOCUS       DAZFKM010000296         2702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFKM010000296 DAZFKM010000000
VERSION     DAZFKM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.299"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            283..1017
                     /locus_tag="ACHKAY_005339"
     CDS             283..1017
                     /locus_tag="ACHKAY_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO5703792.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNNADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACGSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQAAELKARGLLS"
     gene            1014..1514
                     /locus_tag="ACHKAY_005340"
     CDS             1014..1514
                     /locus_tag="ACHKAY_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5703793.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1588..2160
                     /locus_tag="ACHKAY_005341"
     CDS             1588..2160
                     /locus_tag="ACHKAY_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO5703794.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(2488..>2702)
                     /locus_tag="ACHKAY_005342"
     CDS             complement(2488..>2702)
                     /locus_tag="ACHKAY_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703795.1"
                     /translation="YNNKHQLLSVAQKIDASSYKTQWDRSEKLDEMLNKLALKLPKDS
                     LVINKIREAIPDDYKTIFYPHLKIAC"
BASE COUNT          713 a          652 c          705 g          632 t
ORIGIN      
        1 ccatcaacgg gtgaacagtg gtgaacagac ggtgaacagt cattactgcg attgttcacc
       61 ctttaactta ctgtattact tatctttttt cttatggtga acagaggtga acagtaaaat
      121 ataaaaaaac aaacagtaag ccggtttttc ctgcgacctt ttcctggctt gccggtctga
      181 ggatgagtct cctgtgtcag ggctggcaca tctgcaatgc gtcgtgttgt tgtccggtgt
      241 acgtcacaat tttcttaacc tgaagtgacg aggagccgga aaatgtctga caacaccatc
      301 cctgaatatc tgcaacccgc gctggcacaa ctggaaaagg ccagagccgc ccatcttgag
      361 aacgcccgcc tgatggatga gaccgtcacg gccattgaac gggcagagca ggaaaaaaat
      421 gcgctggcgc aggccgacgg aaacaacgct gacgactggc gcacggcctt tcgtgcagcc
      481 ggtggtgtcc tgagcgacga gctgaaacag cgccacattg agcgcgtggc acgccgggag
      541 ctggtacagg aatatgacaa tctggccgtg gtgctgaatt tcgaacgtga acgcctgaaa
      601 ggggcgtgtg gcagcacggc caccgcctac cggaaggcac atcatcacct tctgagtctg
      661 tatgcagagc atgagctgga acacgccctg aatgaaacct gtgaggcgct tgtccgggca
      721 atgcatctga gtatcctggt acaggaaaat ccgctcgcca acaccaccgg ccatcagggc
      781 tacgtcgcac cggaaaaggc tgtcatgcag caggtgaaat catcgctgga acagaaaatt
      841 aaacagatgc aaatcagcct caccggcgag ccggttctcc ggctgaccgg actgtcagcg
      901 gcaacacttc cgcacatgga ttatgaggtg gcaggcacac cggcacagcg caaggtgtgg
      961 caggacaaaa tagaccagca ggcagcagag cttaaggcca gagggctgct gtcatgattt
     1021 actgcccgtc gtgtggacat gttgctcaca cccgtcgcgc acatttcatg gacgatggca
     1081 ccaagataat gattgcacag tgccggaata tttattgctc tgcgacattt gaagcgagtg
     1141 aaagcttttt ctctgactgt aaagattcag gaatggaata catttcaggc aaacagagat
     1201 accgcgattc actgacgtca gcctccggca gtatgaaacg gccgaaaaga atgcttgtta
     1261 ccggatattg ttgtcggaga tgtaaaggcc ttgcactgtc aagaacatcg cggcgtctgt
     1321 ctcaggaagt caccgagcgt ttttatgtgt gcacggatcc gggctgtggt ctggtgttta
     1381 aaacgcttca gaccatcaac cgtttcattg tccgcccggt cacgccggac gaactggcag
     1441 aaagcctgca tgaaaaacag gaactgccgc cagtacgctt aaaaacacaa tcatattcgc
     1501 tgcgtctgga atgagggctg ccggttaaca ccggccgtcg ccgcacaccg tatttttatt
     1561 cttcagcatg atgagaaaga gataacgatg gaaagcacag ccttacagca ggcctttgac
     1621 acctgtcaga ataacaaagc agcatggctg caacgcaaaa atgagctggc agcggccgaa
     1681 caggaatatc tgcggcttct gtcaggagaa ggcagaaacg tcagtcgcct ggacgaatta
     1741 cgcaatatta tcgaagtcag aaaatggcag gtgaatcagg ccgctggtcg ttatattcgt
     1801 tcgcatgaag ccgttcagca catcagcatc cgcgaccggc tgaatgattt tatgcagcag
     1861 cacggcacag cactggcggc ggcactggca ccggagctga tgggctacag tgagctgacg
     1921 gccattgccc gaaactgtgc catacagcgt gccacagatg ccctgcgtga agccctcctg
     1981 tcctggcttg cgaaggggga aaaaattaat tattccgcac aggatagcga cattttaacg
     2041 accatcggat tcaggcctga cgcggcttcg gtggatgaca gccgtgaaaa attcacccct
     2101 gcgcagaaca tgattttttc gcgtaaaagt gcgcaactgg catcacgtca gtctgtgtaa
     2161 aattccccga aaatccgcct gtttttactg aaaaaagcca tgcatcgata aggtgcatgg
     2221 ctttgcatgc gttttcctgc ctcattttct gcagaccgcg ccattcccgg cgcggcctga
     2281 gcgtgtcagt gaaactgcat taaaaccgcc ccgcaaagcg ggcgggcgag gcggggaaag
     2341 cactgcgcgc aagtggtgtt ttataatcaa agacagataa ctgtattatt atattggtaa
     2401 ttaatagttg taacgtaatg tttgttgggt aaagcatttt gtgtaaaaag ccgctactat
     2461 agcggcttga atattggaac tatatattta gcaagcgatc ttcaggtgag gatagaatat
     2521 cgttttataa tcatcgggaa ttgcctctct gattttatta attaccaagc tatctttagg
     2581 taattttaag gctaatttat ttagcatttc atcaagtttt tcactacggt cccattgcgt
     2641 tttataactt gatgcatcta ttttttgtgc aacggataat agctgatgct tattgttata
     2701 aa
//
LOCUS       DAZFKM010000297         2662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFKM010000297 DAZFKM010000000
VERSION     DAZFKM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1215
                     /locus_tag="ACHKAY_005343"
     CDS             <1..1215
                     /locus_tag="ACHKAY_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5703796.1"
                     /translation="KALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDSVQPEPVKV
                     TADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETCDSVNQNQQE
                     SEQNDQKTEQNSPNAQQNEPETKQEPEKACTACGQTGGGSCPDCGAVISDATYQEIFD
                     EENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANGHHNLTSTS
                     RAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAGGVIDRDTI
                     KWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGATFDNVILRRS
                     YERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNALNDARYQAKYVSA
                     IWQKLLPSQADF"
     gene            1283..1525
                     /locus_tag="ACHKAY_005344"
     CDS             1283..1525
                     /locus_tag="ACHKAY_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO5703797.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1503..2522
                     /locus_tag="ACHKAY_005345"
     CDS             1503..2522
                     /locus_tag="ACHKAY_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5703798.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
BASE COUNT          852 a          588 c          647 g          575 t
ORIGIN      
        1 aaggcgctgg ctgccggggc tactgaaatc acgaaagaag aatatcttgc ccgcaaaaac
       61 ggaacataca cggacttacc gcaaaatgtg gataccgctg aagattccgt acaaccggag
      121 ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa tatcagccag
      181 cctgacgccg ataaattgct tgctgcatca cgtggtgaat ttgttgaagg gattagcgat
      241 ccgaatgatc cgaaatgggt gaaggggatt gaaacctgcg attctgtgaa ccagaaccag
      301 caagaatcgg aacagaacga ccagaaaacg gaacaaaaca gcccaaatgc gcaacaaaac
      361 gagccagaaa cgaaacagga gccggaaaaa gcctgcaccg cctgcggtca gaccggcggc
      421 ggtagctgcc ctgattgtgg tgcggtgata agtgacgcaa cataccagga aatattcgat
      481 gaagagaatc agcctgaagt tcaggaaaat gatccggagg aaatggaagg cactgcgcat
      541 cagcacaagg agaacactgg cggcaatcag catcatgcca gcgatagtga aactggcgag
      601 gcgtcagatc ccttaattaa ggcgaacggt catcataatc tcacatccac cagcagagcg
      661 gggattcatc tgatgatcga ccttgaaacc atgggaaaaa atcctgatgc cccgattatc
      721 tcaataggtg caatattttt cgatccgcaa accggagaga tggggccaga attcagcaaa
      781 actatcgatc tggaaactgc tggcggagtc attgatcggg acaccattaa gtggtggctg
      841 aaacagtcac gcgaagcgca atccgccatt cttaccgatg aaatcccgtt agatgatgca
      901 ctgctgcaat tacgggaatt tatcgacgaa aactccggtg aattttttgt tcaggtctgg
      961 ggtaacggtg caactttcga caacgtgatt ttacgccgtt catatgaacg gcaggggatc
     1021 ccctgcccgt ggcgttacac caatgatcgc gatgtaagaa cgatggttgc tctgggactg
     1081 gtgatggatt tcgacgcaag aacgactatt ccattcgaag gtgaacgcca taacgctctg
     1141 aacgatgcac gttaccaggc gaaatacgtt tcagccatct ggcaaaaact gctcccgagt
     1201 caggctgatt tttgatgttc aacccatatc gccgcccacc agctatagtg gcggcggtca
     1261 tgctgtaaag gcacgtgacc acatgtacga attaactcta tctccagcag agattaaaga
     1321 gatcacgaaa tacgagcgat acacaaaaca gcaacaccag ttaagactgc acggcatccc
     1381 atttgtaatc ggccctaaaa acgaacccat cgttctccgc agggatattc cacacggtct
     1441 gacaacgatg ccaaaaacat ctgaactggt ttctgctgaa cccgattttg aggcgctgaa
     1501 caatgggaag accaagaaaa aataaaaaag ataatgtact gccaccgcgg gttagatcga
     1561 atggttacag ttacgtgtgg aaacccgaag gaagtacaag aagtataggg ctaggaagag
     1621 tgcggaaaac cagcgtagct aaagtctggc aaaattatga actggaaaaa gcaaaactcc
     1681 acaacataat gaccgtagct aaattgtggc acatgtttat ggactcccct gcatttacag
     1741 aactggcccc ccgaacccaa aaagattatc gacaacatca gaaggcgttg ctgatggtat
     1801 tcggaaaagt gcttgctgat aatgtcaaaa ctgagcaggt aagaattttc atggataaac
     1861 gagggcttga gagcaagacc caggcaaatc atgaactggc aagcctgagt cgagtatacg
     1921 ggtggggata tgagcgtggg tatgtgaaga ataacccatg caaaggagtc agaaaattct
     1981 ctcttaaagc ccgcactgtt tacatcaccg atgaacagta tgcggcgata tatgcggaag
     2041 caattccaca gttacgcatt gcaatggaga tttcctatct ctgtgcggca agactcggtg
     2101 atgtgcttga gttgaaatgg caggatatta tggataaagg gatctacatt gagcaaaaca
     2161 aaaccggcac caaacaaatc aaggaatggt caccgcgatt acgtacagcg atccagttag
     2221 cccgaaatgt atcttcctgt acatgcgaat atgtgatcaa tacaaccaaa ggcgggaaag
     2281 tcatagctaa aacgctgaat aactggtgga atcaggctaa acgcgcagcc gagcaaaaag
     2341 ttggcgttcc gttcgggtgt aattttcacg acataaaagc caaggggatc tcagattacg
     2401 aaggcagcag tcgcgacaaa caaattttca gcgggcataa aacagaaaat caggtgttga
     2461 tttacgatcg taaaacaaaa atcacaccaa cactggattt gccgctcgtg gttagcaagt
     2521 agacaatcca gaaaaatttc tggcaaaaag acttggtata ctagctatga gcaacttcct
     2581 aataccttga tttttcttgg tgttaaattt gattaacttt tcagcaattt cacgtattac
     2641 actgattttt atttgttttt ct
//
LOCUS       DAZFKM010000298         2659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFKM010000298 DAZFKM010000000
VERSION     DAZFKM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            257..451
                     /locus_tag="ACHKAY_005346"
     CDS             257..451
                     /locus_tag="ACHKAY_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO5703799.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            939..1100
                     /locus_tag="ACHKAY_005347"
     CDS             939..1100
                     /locus_tag="ACHKAY_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO5703800.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHVLTPLAAAIGNCHGGVNL
                     EVMHLLRLN"
     gene            complement(1347..1724)
                     /locus_tag="ACHKAY_005348"
     CDS             complement(1347..1724)
                     /locus_tag="ACHKAY_005348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703801.1"
                     /translation="MNDFNPISQGGSTSGSSASYNPNPNYSLINNRVEKYSDKIGTGD
                     DAKSSVVWFVITFTLTMYACLISALVIVDIFNNKGVNILNNIKESWAVFTPIITLSLG
                     YMFGRVEASHAANKESVKPINKD"
     gene            complement(1724..2086)
                     /locus_tag="ACHKAY_005349"
     CDS             complement(1724..2086)
                     /locus_tag="ACHKAY_005349"
                     /inference="COORDINATES: protein motif:HMM:NF045442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6864 domain-containing function"
                     /protein_id="HFO5703802.1"
                     /translation="MKIEAEVDGKELVYSSSLTMNPNGKLVFNFDNEARITVVFISED
                     TGDNGNRLVKSTVDDDGLGMTISCYNFKMYSPREEGLASEPQFIFTKNNKKHYFNFRT
                     NLFNSESRWISINFLRDK"
     gene            complement(2591..>2659)
                     /locus_tag="ACHKAY_005350"
     CDS             complement(2591..>2659)
                     /locus_tag="ACHKAY_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HFO5703803.1"
                     /translation="YAEVLIAITEYHRALLSRLMAD"
BASE COUNT          852 a          641 c          507 g          659 t
ORIGIN      
        1 tgtcggggca aagccctgac cagggtaatt gtaatagcgt gcatgtatgc gcggtataac
       61 aattacacat cctgtcctgt cagcaagttc gaattaccga caaagacacg gcagtttttt
      121 ggcagtgaca gtattgtcag gcagaaatac ccgtcaaagc gtcagacggc agaataccgg
      181 cggtaaaaca gtcgaaaagt ggtacgcgat ataatcacac aacgcagatg acagacagaa
      241 cggagaaaac gaaaagatga acaagcagca gcaagccgta ctcaacatgg caggattcat
      301 aaaaagccag agcctgacgc tgctcgaaaa actggacgca ctcgatgctg acgaacaggc
      361 cgccatgtgt gagaagctgc acgaactcgc ggaagaactt cagaacagca tacagacacg
      421 ctttgaagcg gaaaatcgca ccgggatata acgcatccgg acaggatgca gaacggcata
      481 gaggagcgta aggagaaaat gtcacataag gcgctcttcc gcttcctcgc tcactgactc
      541 gctacgctcg gtcgttcgac tgcggcgagc ggtgtcagct cactcaaaag cggtaatacg
      601 gttatccaca gaatcagggg ataaagccgg aaagaacatg tgagcaaaaa gcaaagcacc
      661 ggaagaagcc agcgccgcag gcgtttttcc ataggctccg cccccctgac gagcatcaca
      721 aaaatcgacg ctcaagccag aggtggcgaa acccgacagg actataaaga taccaggcgt
      781 ttccccccgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc
      901 tcggcccggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      961 ccgactgctg cgccttatcc ggtaactatc gtcttgaatc caacccggta agacacgtct
     1021 taacgccact ggcagcagcc attggtaact gtcatggagg agtaaatctt gaagtcatgc
     1081 acctgttaag gctaaactga aaaaacaagt tttggtgact gagctcctcc aagccagtta
     1141 cctcggttaa aagagcgcca actcttttaa ccttcgaaaa accgcctgcc aaggcggttt
     1201 tttcgtttac agggaatgag attacgcgca gaaaaaaagg atctcaagag gatccttgca
     1261 tgtcttacct ccccctaacg ataaagcaag ccatcaagtt catgaatgaa catattccta
     1321 tccttctctc aaaaaataag aaataattaa tccttgttga tgggttttac actttctttg
     1381 ttggccgcat gcgatgcctc aaccctacca aacatataac ctagggataa tgttattatt
     1441 ggcgtaaaaa cagcccatga ttcttttata ttattaagaa tattgacacc tttattgtta
     1501 aagatatcaa caataacaag tgcagatatt aaacatgcat acatagtcaa tgtaaatgtt
     1561 atgacaaacc aaacaacact actttttgca tcatcaccag tccctatctt atctgagtat
     1621 ttctccaccc gattatttat taaagaataa tttggatttg gattataaga ggccgaacta
     1681 ccagaggtag atccaccttg agaaattgga ttaaagtcat tcattactta tccctcaaga
     1741 agttaatact aatccatctc gactcagaat taaaaagatt tgttctaaaa ttaaaataat
     1801 gctttttatt attttttgta aagataaact gaggctctga agccaatcct tcttctcttg
     1861 gactatacat tttgaaatta taacaagaga ttgtcatccc caatccatca tcatcgactg
     1921 tagatttaac aagcctattg ccattatcac ctgtatcttc agaaataaaa acaactgtaa
     1981 ttcttgcttc attatcaaaa ttaaaaacca attttccatt aggattcata gtcagagaac
     2041 tagaatatac cagctctttt ccatcaactt cagcttcaat tttcatatta tctcatctta
     2101 taaacaataa taaccaacac agaaaaaacc aaaacaataa acccaggcat tacaacaacc
     2161 cgatgaagtt tatgtaaaaa cattgactta attagaccat gcataacatg ctttaaaagc
     2221 agaaataaga tcacaagaag tataattatt atcaatgtta aaaaaaccac cgaataattc
     2281 acttatcccc ccactctcct ttgcctcatc aataaaaaac agaacaatta tacatcaaaa
     2341 caaacagtta catccccttc actccaatac ttttaacata atcccttaat tgtacgcacc
     2401 gctgaaacgc gctgaacgca attctgacgc aaataccgca aaaagagagg tttagccccg
     2461 aaaaaacagg aaggatgcgt caggatcgtt ttcagctcac tgcatagcta tgcatgaaag
     2521 cgaatggtga tcactttggg agcttacggt gttcataccg tctgttttcg acagttttct
     2581 ccccgggaag ctaatctgcc ataagcctgg ataacagggc acggtgatac tccgtaatag
     2641 cgatcagtac ctccgcata
//
LOCUS       DAZFKM010000299         2631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFKM010000299 DAZFKM010000000
VERSION     DAZFKM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            465..608
                     /locus_tag="ACHKAY_005351"
     CDS             465..608
                     /locus_tag="ACHKAY_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO5703804.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            621..830
                     /gene="cspE"
                     /locus_tag="ACHKAY_005352"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             621..830
                     /gene="cspE"
                     /locus_tag="ACHKAY_005352"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO5703805.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            996..1805
                     /gene="rlmA"
                     /locus_tag="ACHKAY_005353"
     CDS             996..1805
                     /gene="rlmA"
                     /locus_tag="ACHKAY_005353"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO5703806.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1802..2368)
                     /gene="mntP"
                     /locus_tag="ACHKAY_005354"
     CDS             complement(1802..2368)
                     /gene="mntP"
                     /locus_tag="ACHKAY_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO5703807.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          698 a          617 c          626 g          690 t
ORIGIN      
        1 aagcgtggta ttatttatta gcgaaacgtt tctctcttga ttttttgctc attcatcaat
       61 ttttcttatt ttaaattttc aatccctttg gggattgact tctctttagg gtaattgata
      121 gccgttaact gactgtttta tgagaaaaag tgatataact ttttattcat tgcatagcaa
      181 aaaatgtgat attgcacgca ctatgtaata acttctccca ctggcctgga acaactgaac
      241 ttattgaact atgttagaaa atacgccagt ttaagtatct gcctgaactg gcaaggttaa
      301 gcacaatgat atatcggcgc gtattccgtt gcataagtgt gcaaaaaaag tggaagacgt
      361 atcgagattt gtgcgtctga tcgagacatg tttaaaaatg gcttgccata attaacgttg
      421 tatgtgataa cagatttcgg gttaaacgag gtacagttct gtttatgtgt ggcattttca
      481 gtaaagaagt cctgagtaaa cacgttgacg ttgaataccg cttctctgcc gagccttata
      541 ttggtgcctc atgcagtaat gtgtcagttt tatctatgtt atgcctgcgg gcgaagaaaa
      601 caatctaagg aatttttcaa atggcaaaga ttaaaggtca ggttaagtgg ttcaacgagt
      661 ctaaaggttt tggcttcatt actccggctg atggcagcaa agatgtgttc gtacacttct
      721 ccgctatcca gggtaatggc ttcaaaactc tggctgaagg tcagaacgtt gagttcgaaa
      781 ttcaggacgg ccagaaaggt ccggcagctg ttaacgtaac agctatctga tcgaatccac
      841 tgatctgaag tgtgaatacg cttcaatctc gctataaagc ctcgtcgaat gcgaggcttt
      901 ttactatgct ttatcttcgc tcctggcgtt cggatatttg cccgccgcgt gattcgcgtt
      961 acacttgcgg cctttagtat cctgccggag ttgtcatgtc gttttcctgt ccactttgcc
     1021 atcagcctct ttcgcgtgaa aaaaacagct atatctgtcc ccagcgacat cagtttgata
     1081 tggcgaaaga agggtatgtc aatctgctgc ccgttcagca taaacggtct cgtgatccgg
     1141 gcgacagcgc ggaaatgata caagcacgcc gcgcattctt agatgccgga cattatcagc
     1201 cgctgcgtga tgcaattgtc gcccaactgc gggaacggct tgatgaaaag gccacggcgg
     1261 tgctggatat tggctgtggt gaagggtatt acacacacgc atttgccgat gtgttgcccg
     1321 aaatcaccac gtttggcctg gatgtctcga aggtagcgat aaaagcggcg gcgaaacgct
     1381 atccgcaggt cactctttgt gtcgcttcca gccaccgttt gccgttttcc gataccagta
     1441 tggacgccat aatacgtatt tacgcgccgt gtaaagcaga agaattagca cgagtagtga
     1501 agcccggcgg ctgggtcatt actgccacgc cgggaccgcg acatttgatg gagctgaagg
     1561 ggctgattta caatgaagta catcttcatg cacctcatgc agaacaactg gaaggtttta
     1621 cattacagca gagtgatgag ttgtgttatc cgatgcgtct tcgcggtgat gaagccgtcg
     1681 cattattgca gatgacgccg tttgcctggc gtgcgaagcc agaagtctgg caaacactgg
     1741 cagcaaaaga agtgttcgac tgccagacgg actttaatat tcacctctgg cagcgttctt
     1801 attaaccgtg gaagtgcgtc cagaggatct ggacgccgat gccgatcagc accagcccgc
     1861 cgagaatttc cgcttttttc ccaataattg agccgataaa gcgaccaacc atcatcccta
     1921 atgtcgacat aatcaaggtt gcacaaccaa tggccaatgc ggtcgcgata atgttgacct
     1981 gcaggaaagc aagaccaaca cccacagcca tggcatccag gctggtggca atcgcggtgg
     2041 ttaccagtag ccagaaaccg tgccgacggc gcggctcttc atcttcatca tctgcgccac
     2101 gaaaaccctc aataatcatt cgcccgccga ggaatatcag cagcacaaac gcaatccagt
     2161 ggttccattc aaggacaaac cggctggcta acatgcccat tccccagccg atcagcggcg
     2221 tcagggtttc gacggcacca aaaataaggc cggttcgcaa tgcttcagaa aatttcggtt
     2281 tatggagggt ggcaccttta ccgattgatg cagcaaatgc atccatcgac ataccaaacg
     2341 caagaagaac agtagcagtg atattcatga caatgtcctg accggggtat ccataaaaca
     2401 catcatcgcc cccagcaaac atgcatacat gaacaacaag ccgcgatggt tttcgcgttc
     2461 atgcaggcat agcgattgat gtgcatatgg tctcgcctga ctgaaagtat tcagtccgta
     2521 cgtgccacgt tttgcaacga gtatgttgac acgtacattt ccggaatctg gaaatcggct
     2581 actccccaaa tgagggcgca accttaacat attttgagaa tataaaacaa c
//
LOCUS       DAZFKM010000300         2621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFKM010000300 DAZFKM010000000
VERSION     DAZFKM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..1120
                     /locus_tag="ACHKAY_005355"
     CDS             161..1120
                     /locus_tag="ACHKAY_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO5703808.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(1264..1335)
                     /locus_tag="ACHKAY_005356"
                     /pseudo
     CDS             complement(1264..1335)
                     /locus_tag="ACHKAY_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(1332..1520)
                     /locus_tag="ACHKAY_005357"
     CDS             complement(1332..1520)
                     /locus_tag="ACHKAY_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO5703809.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1517..1879)
                     /locus_tag="ACHKAY_005358"
     CDS             complement(1517..1879)
                     /locus_tag="ACHKAY_005358"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5703810.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(1876..2166)
                     /locus_tag="ACHKAY_005359"
     CDS             complement(1876..2166)
                     /locus_tag="ACHKAY_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO5703811.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(2166..>2621)
                     /locus_tag="ACHKAY_005360"
     CDS             complement(2166..>2621)
                     /locus_tag="ACHKAY_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5703812.1"
                     /translation="LVDRWRELEETAVNIPKTLPEALRLAADLAEQKMQLENQLAIAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
BASE COUNT          734 a          584 c          556 g          747 t
ORIGIN      
        1 aaacaccgtt acattaaaag ataataaaaa ccgcaataaa aaacgaataa atcaattgtc
       61 tcacgcaatt ataaaacatc atattgatta cgcaccttgt attacaaact catgtatgta
      121 aaatacgcgc accattcaaa aaaaaggaag acaataacat atgaaaaaaa gtgtcatcgc
      181 tggcgtcttt attgctctgt catttaccac gtgttcagct atcgcgaaca gccttgcatt
      241 atcattagca aatgatgatg cagggaagtt tcaaccaata cttaatgata tttatggcaa
      301 taaacatgaa aacagagatg attactcaca aggcttattt ctgggatata gccacgatat
      361 ctcagactcg agccaattat ctctccatat tgcgcaagat atttactctc catcaggcag
      421 taataaaaga cacaacacag ctgtaactgg agacagagct tttagtgcat acactcacac
      481 tggtattgaa tggaactccc ttgcgaatga ctggattcgc tatcgattag gtactgacat
      541 aggtgttgtt ggccccgacg caggcggtca gaaagtacaa aataaagctc atgagattat
      601 tggggcagaa aaatatcatg catgggatga tcaaatagag aatcgctacg gttatactgt
      661 aaaagggatg ctatccatga caccaagtat ggatatttta ggtgctaatg ttggattata
      721 ccctgaagtt tctgctgtta ctggaaactt atttcaatat gtagcatatg gcgcaaccat
      781 tgccattggt aatgataaaa ccttcaattc ggataatggc tttggtctgc tggctccccg
      841 tggtttaatg catatgtccg atacaagcgg attcaaatac aagatttttg caggtatgga
      901 aagacgagat gtcaatcgca actatactct cgaaggaaaa acaatacaga cgaaacaaac
      961 aacagtatcg ctaaacaaaa ctgttgatga atatcaagtt ggcgcaacaa ttgggtatgc
     1021 acctgtagcc ttcacactag catttaataa agtaacatca gaattcaaga caggggatga
     1081 ctattcattt ataaatggag caatcacctt ctttttttaa ctgaattgaa ttcaatcaaa
     1141 ataacataag tccaacaaaa acataaagtg cgaaatgaat gccagctcca tttatttcgc
     1201 actataaaag attaaaagtt gcaataaaat aataaaatga ctcagttacg aaaaccaata
     1261 aactgtggcc agtagtgagt cgctcatcat cgggcttttt ggcgaatgaa atttagctac
     1321 gctttcgagt ctcatcgtct tcccctcttg ccctgtttga ccatcaggac gccgttaact
     1381 attacatgac gctcgccttt gctgtctcgg ttgtacttga gcactgttcc tcttgcgcag
     1441 gaaagcatcc tcgccacttc ggtctgattg cctcgtgtct ggataagaag ctctggtatc
     1501 gtttgaattg tggcgttcat gcgttctcca gttcggtgat ttttattcca agccgtccgc
     1561 ctggtacttt cacaccacga attacgcgaa tgtcatcgaa ttgctcgtcg tcttccgcaa
     1621 atccggcatg gataagggag tcgagtaaac ccttcaggat gttatctagg tcgcggcggc
     1681 gggagtctgg aacgtctgcg attactttga tgcggagtcg tgatttggtg aaaatatcta
     1741 acttgagttg gtggatgatt tgctgaacgt cttttcggta tttctggcct ttatcgctga
     1801 tgtagtattg gcttccccgt cttcgccagt aggtattcac cgacggcggg tatggaagca
     1861 caaactgata ttcgttcatg acttaatctt cccctccttc agcagtatcg cctgcgtcct
     1921 gatcacgcct tcgaggtggt aaagtctggc gtctttgttg tcgaggttat gggtgcgtcg
     1981 gtcgatttca tcgtgacacg cgctacaagc ccatgcgccg atcaggtcgt caggcttcat
     2041 tcccgttccg caaattccag ccatccggta atgtgccaga actgtagttt caggattgcc
     2101 attgcatatg ccgtaaatac gtacctggca ttctctgccg cgagattctt tgcgtaggtt
     2161 agccattaag cagcctcccc tgttactttc agcattccgt tatcgagcag ctttctggtc
     2221 agccactgtt gaccacgccc ggtgattttt gtggtgaacg atatctgtat tccgtgattt
     2281 gtgttgaccg ctgtttcttt cactgtgaaa tagccgcgat ccatatattc ctgcattggc
     2341 acattgcgcc gggaacctga agcaataagg attttgtgat cgcgcatcca cgcaaacagt
     2401 ttgtttggac caataccaac aacctttgca aagtttccaa tcaaaattcc gctggcctcg
     2461 ccaacgcgat cggcaaactc aactttaggt gcggcaattg cgagctggtt ttccagttgc
     2521 attttctgct cagcaagatc agcagcaagg cgcaacgctt ccggtaacgt tttggggata
     2581 ttaaccgcag tttcttcaag ctctcgccaa cggtcaacaa g
//
LOCUS       DAZFKM010000301         2598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFKM010000301 DAZFKM010000000
VERSION     DAZFKM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /locus_tag="ACHKAY_005361"
     CDS             <1..450
                     /locus_tag="ACHKAY_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFO5703813.1"
                     /translation="GGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLAVMATNGM
                     RTSHHIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGY
                     GRVMVVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            455..766
                     /locus_tag="ACHKAY_005362"
     CDS             455..766
                     /locus_tag="ACHKAY_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO5703814.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTECPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            865..1083
                     /locus_tag="ACHKAY_005363"
     CDS             865..1083
                     /locus_tag="ACHKAY_005363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703815.1"
                     /translation="MNRKQKQELKYFLRKEIARLEDAESQSSEIPFGMDINDARMLQA
                     YRIAQAALQTKPLNNIHEKYDYFTDTAC"
     gene            complement(1753..>2598)
                     /locus_tag="ACHKAY_005364"
     CDS             complement(1753..>2598)
                     /locus_tag="ACHKAY_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5703816.1"
                     /translation="ARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSALALDWFVFG
                     NAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPGKVCHLMNPD
                     INQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQVNRESMDSLK
                     ETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASRDDVLAAHRV
                     PPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVIRFNPYALLD
                     TQPTS"
BASE COUNT          685 a          661 c          699 g          553 t
ORIGIN      
        1 ggcggaacca cgctggatgt ggcaaaggtt caggggcaac tggcaggtat cagccaggtg
       61 ttttgcgatc cacacgtagg cgtttctctg atggccgatg ccgtactggc ggtgatggcc
      121 actaacggta tgcgcaccag tcaccacatc gccaatacca ttatcgaaca tcgccatgat
      181 gaagcctggc tgcgccagca catccacaat gacgcgcatt acgccagcct gatggcggtt
      241 attcgtgaaa aggaagaaac actgaaacaa cgcgtgatcc gcgcgctggc ggttttttcg
      301 ggttacgggc gggtgatggt tgtcggtgga ggggcggaga ttgtggcacc cgctatccgc
      361 gaagcctgcg gagttaatgc gactttcatc gcggacgggg tgccacagtt tgctctggtt
      421 aatgggctgt acgcaatgga caaggagtaa accaatgacg acaccaacca gacggataag
      481 tttctatctg aagcctgcgg ccgtcaagaa cgaaagcgaa gcatgcgcct ggctggacag
      541 ccttacacca gaagcccgca aaagcggcca acgcgtggct tttctggccg ggctggcact
      601 tctgaaaatg aatccagcag aggcttaccg actggctgca tgggctgacg atgaggcgtt
      661 atcagtgaca caaaccagga cagaatgccc cgcgtcacag ccagtatcaa ccgcacagat
      721 aaccagtcag atggccggaa atatccgggc attatttccc gaataacaca acatcagggc
      781 gcatccgccc tgatgacttt aatccgggaa cataaacaag ggggacaaaa tgcaacacat
      841 tgacagagaa aaaggaaaaa aagcatgaac agaaaacaga aacaagagtt gaaatacttc
      901 ttacgtaaag aaattgccag gcttgaagat gcagagtcac aatcatcaga aattccgttc
      961 ggaatggata tcaacgacgc ccgtatgctt caggcatacc gtatagctca ggctgcacta
     1021 cagacaaaac cgttaaataa catacatgaa aagtatgatt attttacgga taccgcttgc
     1081 tgacatctga agaacaaaag atagcccaac ttttaggcga cgcatggaac ctgtatttaa
     1141 cattgccagt tgagcatcca atgggtagag atgaattttg ccgggcaatt catcattgcc
     1201 aaaatatgat gttggcacgc ccggccatca ggacgctggc caggaagggg caaggctata
     1261 aaagataagt aaaacgccct cctccatggc gtttatttca atgcacaata gtgcacaaat
     1321 ttgcacaatt tttttgaacg actttttgcc cttccggccc gcgttgcggc tggatccgtc
     1381 aaggatccgt gcgtgcacaa aaaaacgcgc tttttctgcg cgcaggtgac gggggaacag
     1441 cccgcgtttc agggggtaaa tagcattccc tgaacgatgt cgcagcgaca caacagaatg
     1501 gctgtatttc tcacgctgag cgtgaaaaag gcgtgagggc ttttgatttg atggggtgaa
     1561 aggtaaggcc gtcaaaatcg cactgaggcg gcgagaacat acagtcaacg cggtgggatt
     1621 gcgtaagagt ctgaccgtcg atggtggcga taaactggaa ggcgtcgtga aattatctga
     1681 ctgatacagg agctggagag tcggggcata aattttttat gccccggcga agcagcagac
     1741 aagcgaagcg cgtcaggatg tgggctgggt gtctaacagt gcgtaagggt taaagcggat
     1801 cacctcttcg ccaagccagt cattgatgtg cttcatggcc tccatgacgg gcatcagctc
     1861 gttaattgcg taaacccgcg cagccttctc cacatcacca aacgcacttt tttcgcccgg
     1921 catcgccccc atcagttgcg gcggaacgcg gtgcgcagcc agcacatcat cacgggatgc
     1981 cgccttaaca ttcatgaact catcctttgc ggtgatctgc tggaacggca aaatttgcac
     2041 cccctctttg cccccgttgg gcgcatggat gagcacgttt ttaaacgcac caccaccacg
     2101 cgcaccctgt aacgtttctt tcagggagtc catgctttcg cggtttacct gcgctgcacc
     2161 gatgtagatg atgcacccgg cgtgggatcc attgtcgtaa tacagttttc tgaacatgtc
     2221 cgccgaatga gacaggctgg ccgagagtaa tgcgccaaga tattccggca tgccgtaaat
     2281 ttcctggtta atgtccggat tcatcaggtg gcacactttg ccagggcgaa actgaaacgc
     2341 gtccttgcca tcctgcacat accaccatga ttcaagatcg cttccgcgtc gcatgtattt
     2401 cgccagtgcg tgccgtaatt taagtggttc gccgagcata ttgctccgaa gctcaaggaa
     2461 tgcgttaccg aacacaaacc agtccagcgc cagcgccgag aaatcctgcc gggaaagcag
     2521 cgggtgcggg atgtagcaac cgagcaatac attgcgctta aagtaaagcg cagactgatg
     2581 ccaggacgtt tgccgggc
//
LOCUS       DAZFKM010000302         2562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFKM010000302 DAZFKM010000000
VERSION     DAZFKM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2115
                     /locus_tag="ACHKAY_005365"
     CDS             <1..2115
                     /locus_tag="ACHKAY_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5703817.1"
                     /translation="ELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSI
                     DAASRTLTLDREVTLPETGTPTVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVETY
                     GVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNS
                     VIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITA
                     DTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPAGVELTPGYFQITAV
                     PKLTIYDPTVQFEFWFSEAKIADAAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVR
                     SVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMK
                     TSITETRNEITQTVSKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVA
                     GIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAV
                     SITSSGNPPAFSLTPDGRLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIE
                     GDIVKTVSKSFPRTSTYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQS
                     YWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTP
                     TTSISDLLVVVMKKATAGISIS"
     gene            2183..>2562
                     /locus_tag="ACHKAY_005366"
     CDS             2183..>2562
                     /locus_tag="ACHKAY_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5703818.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTHYSDTRWHEDFVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVST"
BASE COUNT          620 a          665 c          754 g          523 t
ORIGIN      
        1 gaactgctgg aaacgcagac ggtggatttc acgctcgggt cacaggggct gcgtcacaca
       61 cccggtgaca ttattgaaat ctgtgataac gactatgccg ggaccatgac cggcggacgt
      121 gtcctgtcca tcgatgccgc cagccgcacc ctgacactgg accgtgaggt gaccctgccg
      181 gagacaggta caccgacggt gaacctgatt aacggcagcg gtaagccggt gagcgtggcc
      241 atcactgcac accccgcgcc ggaccggata caggtcagca ccctgccgga tggcgtggag
      301 acatacggtg tatggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtc
      361 tccatccggg aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa
      421 aaagaagcca tcgtggataa cggggccagc tttgagccgc agtcaggcac cctgaacagc
      481 gttatccctc cggcagtgca gcacctgacg gtggaggtga gcgcggctga cggtcagtat
      541 ctggcacagg cgaaatggga cacgccgcgg gtggtgaagg gcgtgcgctt cagtctgcgc
      601 ctgaccagcg gaagcggtga gaacagccgc ctgctgacca ccgccatcac tgccgatacg
      661 gagcaccgtt tcagtggcct gcctctcggg gaatacaccc tgacggtcag ggcgattaac
      721 agctatggcc agcagggcga acccgccacc accacgttcc ggattaacgc accggcagcg
      781 cctgccggtg ttgaactgac gccggggtat tttcagataa cggcagtccc gaaactgacc
      841 atttatgacc cgacggtgca gtttgagttc tggttttcgg aggcaaaaat tgcagacgcc
      901 gcacaggtgg aaacctctgc ccgttatctg gggaccggca gtcagtggag tgtctccggt
      961 ccgcacatta agcccggaaa ggatttctgg ttttatgtgc gcagcgtcaa cctggtgggg
     1021 aaatctgctt ttgtggaagc cagtggccgg gccagcaatg atgcagaagg gtatctgggg
     1081 ctgtttcggg aaaaaatagg aaaactgcat ctggctcagg ggctgtggga gctgatagac
     1141 aacagccagc ttgcggatga gatggcggag atgaagacca gcatcaccga aacccgcaat
     1201 gaaatcacac agacggtcag taaaacactg gagaaccaga gcgccaccat acagcagata
     1261 cagcgcgtgc agaaggacac aaatgatgac ctggctgcgc tgtacatgct gaaggttcaa
     1321 aaaacgaaag acggcattcc ctatgtggcc gggattggtg cagggattga ggatactgat
     1381 ggccagccac tgagcaacat actgctgctg gctgaccgta tcgcgatgat aaatccggag
     1441 agcggcaaca gcacgccgtt atttgtggcg caggggaatc agctgttcat gaacgacgtg
     1501 ttcctgaaac gactgtttgc ggtgagtatc acgtcatccg gcaatcctcc ggcattttcc
     1561 ctgacgccgg acgggcgact gacggcgaaa aatgcggata tcagtggcag tgtgaatgcg
     1621 aactcaggga cgctcaacaa cgtcacgatt aatgagaact gtcagattaa ggggaaactg
     1681 tcagccaacc agattgaagg cgatattgtc aaaacggtca gcaagtcttt cccccgcacg
     1741 agcacttatg ccagtggcac catcacggta agaatcagtg atgatcagaa gtttgaccgg
     1801 caggtcatga taccgccagt gttattccgc ggtggtaagc atgagaattt caacagtaat
     1861 aaccaacagt catactggta ttcaacctgc cggttaagag tgacccgcaa tggtcaggag
     1921 atttttaatc agtccacgac ggatgctcag ggcgtatttt cctcagttat agatatgcct
     1981 gccggacagg ggacgctgac actgacattc accgtatctt catcaggagc gaataactgg
     2041 acaccaacaa ccagtatcag cgatctgttg gttgttgtga tgaagaaagc caccgcaggc
     2101 atcagtatca gctgaatttt ataacccata tacgggcgcc agaaatggcg ccttttttat
     2161 tgcagaaaag cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat
     2221 ttgtctggcc gtatccggtg cgcctgcatg ggcatctgaa catcagtcca cgctgagcgc
     2281 ggggtatctt catgtctcga cgaacgttcc tggcagcgat gaactgaacg ggattaacgt
     2341 gaaataccgt tatgagttta cggacacact ggggatggtg acgtcgttca gctatgcagg
     2401 agacaggaat cgccagctga cccattacag cgatacccgc tggcatgaag atttcgttcg
     2461 taaccgctgg ttcagcgtaa tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc
     2521 gtatgcgatg gcgggcgtgg cttacagccg tgtgtcgact tt
//
LOCUS       DAZFKM010000303         2543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFKM010000303 DAZFKM010000000
VERSION     DAZFKM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..269)
                     /locus_tag="ACHKAY_005367"
     CDS             complement(<1..269)
                     /locus_tag="ACHKAY_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703819.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIEFIYS"
     gene            complement(281..973)
                     /locus_tag="ACHKAY_005368"
     CDS             complement(281..973)
                     /locus_tag="ACHKAY_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5703820.1"
                     /translation="MINQLTFTKHYDTFDNVSKIYSDKFPQGKDLDLLHIVLYFRFLS
                     YQENNLNCYESHETLAKIFKSSASTIKRKINDLKEMGLLETSPHPDPYISSLIYNALP
                     LTDAHITPPGESSLSDLSEAEEAQEQPKGHKQPSFDVLDDDWDTPLPWETEETPVSSS
                     EKVANDNEEDVLKNFASLIIKQKNTRTGGASFIEFATSLAYKHRLKLPNGIEAYFAKK
                     HPKVYEDFDIPF"
     gene            complement(1027..1197)
                     /locus_tag="ACHKAY_005369"
     CDS             complement(1027..1197)
                     /locus_tag="ACHKAY_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703821.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(1217..1405)
                     /locus_tag="ACHKAY_005370"
     CDS             complement(1217..1405)
                     /locus_tag="ACHKAY_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703822.1"
                     /translation="MKWFTPEHVVQAFKKGELTRHQVVMNRNMARSRGYPERAACFNE
                     ALKIIDELRKNEKESETE"
BASE COUNT          750 a          465 c          463 g          865 t
ORIGIN      
        1 gaatagatga attctatttc tttatctagt cttttgagat tgaatgtaga gaaagataat
       61 tctttaattt ctataacacc gaagtttgat agctgtttca gccagaattt taaaccatct
      121 tcagagcacc ctgctcgatg taacacatcg ctatatttta gcggtgttgg ctttccttta
      181 tagaggtaag caagtgtttc gtagatgcgt tttcctgtgt cgttacactg aattctgtct
      241 acttgatttt ttactaaata ttgaatcata accctcctta ttaaaatgga atatcaaagt
      301 cttcgtatac tttgggatgt ttcttagcaa aatatgcctc aataccattt ggtagcttta
      361 acctgtgctt ataggctaac gatgttgcaa attctataaa tgatgctcct cctgttctag
      421 tattcttctg cttgataata agagaggcaa aattctttaa cacatcctcc tcattatcat
      481 tagctacctt ttcacttgat gaaacaggtg tttcttccgt ctcccacggt aaaggcgtat
      541 cccaatcatc atcaagaaca tcaaaagaag gctgtttatg gcctttaggt tgttcctgtg
      601 cttcttctgc ctcagaaaga tcagagagtg atgattctcc tgggggagta atatgtgcat
      661 ctgtgagagg gagagcattg taaatcaaag atgaaatata cggatcagga tgtggggatg
      721 tttctaataa tcccatctct ttaagatcgt ttatcttacg tttaattgtt gacgctgaag
      781 acttaaaaat cttagctaat gtttcgtgac tttcataaca attaaggttg ttttcctgat
      841 aactaaggaa tcgaaaataa agcacaatat gtaatagatc taaatctttt ccttgaggga
      901 atttatcaga ataaatcttt gatacattat caaacgtgtc atagtgtttt gtaaaagtta
      961 gttggttaat cattaaaaat ctccttgatg taatcacgct ctatggcgct actatggtga
     1021 taaaaatcac ttgcttttat ttgagagtgt ttcagcctct cttttcaaca tatcaagaat
     1081 gtaatctgtg acagtgatcc gtctacttct ggcttcttta aataacacct gaaatacttc
     1141 atcaggaata tgaaagcaag tgcgtggttg tcttttcttt tctgtagttt cgctcatagt
     1201 tttctccttc tcttctttac tctgtttcag attctttttc attttttctt agttcatcaa
     1261 taatcttgag tgcttcattg aagcaagctg ctcgctctgg ataacctcga ctccttgcca
     1321 tattacgatt cattactact tggtggcgtg taagctcacc ttttttaaat gcctggacga
     1381 cgtgctcagg tgtaaaccat ttcataaaaa actccttatt cagttaaaag aaaaaacacc
     1441 ttgcaagcct gacggcttca aagtgcattg attttgatta tttagagaag aatgtttttc
     1501 tgtccctcta atacctatta ttacatataa agaaacattt gtcaatacct tttcataaaa
     1561 aatctgatct ttttcatgca cttacatttt ttcttagcaa ttcgtgctct cctatgtagt
     1621 gctattcttc cttattgtct aaaacgctct aaaacgcgtt ctaacgagat tttgataaaa
     1681 gcaatacatt cccttatcta atggctgttt agctcgccag aggcttattt aagtggctta
     1741 ttttgtattt gttagatgat gatagatata aaacagagag tcactttata aaacgctctg
     1801 taaagccctg tagtgagttt attatatgta ggcaatgcgt ttccattccc taccctgctt
     1861 aatgttgtta gagaagcatt tagacccctt atttttaatt acctaaagta tcctctatac
     1921 atctctgtta tgaaacacct ctgaatcctt cataaaacac attatgacgc aaagaacact
     1981 tttctaaggt gattcccttc ctgaatgctg tttcttgcgt cagagagcta cacagagctt
     2041 ataaagatat tattaataat aaagtagata aagtaagtaa gataagtaag taagataact
     2101 aagatagata agattaataa gatcagaatt ctctttcggt gtcgcctctg gcttttccac
     2161 cgaaaacaat tacacgacta cagaatgtag gaagtgaaat tgtattgagg cttagtgaaa
     2221 ttacacgagg aaagcagagt gtaattgagc tttagccgct ctcttcgagt aattacacaa
     2281 ctacagagaa taacgagagt tattaaatgt aggaagtgga attaagaggt agagagttac
     2341 acataagcaa tcagtatgat tggttaggtg attactcttc tgcgcacagc gcaatagtat
     2401 ttcttcgtgg gcgttaccgc cacgatagtt acacgaagaa agtaacgctt tgctttcggt
     2461 gtgagcggaa cgggccagcc acgaagcccg aaaggtagcg gagtaaggct gcttgccaga
     2521 gagtgaagct aacgccggaa ggc
//
LOCUS       DAZFKM010000304         2529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFKM010000304 DAZFKM010000000
VERSION     DAZFKM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..823)
                     /gene="cif"
                     /locus_tag="ACHKAY_005371"
     CDS             complement(<1..823)
                     /gene="cif"
                     /locus_tag="ACHKAY_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO5703823.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKTL"
     gene            complement(1047..1928)
                     /gene="nleH"
                     /locus_tag="ACHKAY_005372"
     CDS             complement(1047..1928)
                     /gene="nleH"
                     /locus_tag="ACHKAY_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO5703824.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(2034..2303)
                     /locus_tag="ACHKAY_005373"
     CDS             complement(2034..2303)
                     /locus_tag="ACHKAY_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703825.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(2305..>2529)
                     /locus_tag="ACHKAY_005374"
     CDS             complement(2305..>2529)
                     /locus_tag="ACHKAY_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703826.1"
                     /translation="RFRLGPGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQGFF
                     QHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          692 a          628 c          453 g          756 t
ORIGIN      
        1 tcaatgtttt ttcaatatta tcggtgtcat actcaaacaa ctggaaacta aaattgtcat
       61 gcttttgata ttgaatattt tcctttcgta acattttaac atttccatca atatcaaata
      121 acgttgcaat ctgctccaaa tctgtcttat tttgagacat gcaggagaag taattacaaa
      181 tatcatccag caaaatcgga gtcaatgctt tatgcgtcat ccagtcctca aatcttaact
      241 ttctggtgac gccctctcct aaatctgact gatacaagaa tgcaggacga caagagtttt
      301 ctcctttggg aatatcgaca atgtaagcat gaccaagcgc accatcatta acacgtaata
      361 caaagctctt acattcaggc atacaggaca ggcgctcctg aagctgctcg agcgatagtg
      421 tctgttcatt ttcaaagcag taactgaagc catccttgtc cagaaatagt ttcataatag
      481 cattagcggt aactccgcag gagggttcca caatgccttt ctctcgccct ataataacct
      541 caacctcctc aatcgacatc ccccagtctg ttgccatctg tttgatgtta gagtcgatat
      601 tttgcattat tgcagcagga tttttctgat gtataggtac ttcgtggtca tcactattta
      661 gcaatacatt agctttgagg ttctgtgagc acagggaagc aaaatctctt acatgtaagg
      721 ctgaagtgtg ttgcatggga gataatacag cttcagtatt tacagatatg gcccctgtca
      781 gacaggacgc ggacgtcggg gggggaaggg taatgtcttt catatctcat tcattcactc
      841 tatatttatc tataaataca ggcgaggtgc aaaaagaact tctggcatgg gcattatgtc
      901 atgtctgaaa tttttctggt gcctgtatgc cattcgccag tctctcagcg gcattgtcca
      961 tttctgtgac gcggcctgga ttgccagcca caccactttt taccacctcc atccatgcaa
     1021 caaatccact acactggata aaattactaa attttactta ataccacact aataagatct
     1081 tgctttcctc catgataaga ttgcattatt tgattttcac tccatgaacg ttcagaaata
     1141 ttataagatg atatatctat tggattaaat tcatttctcg tgcgatcata taagacattt
     1201 gtctctgttg tatcgataaa aagaattcct ttttgctcaa gcctgtcaaa catatcataa
     1261 atggcatgct cagcctgtgc cggcaaggac gaaatattta aaagcgattc tccatttatt
     1321 ttatccattc taataccaat aatatcgcca ttatcgccat atattttttc tgcactcccg
     1381 gcaccataat attggttgaa gcaacgaact tcgtttgtta cctcttcatt gctttgagat
     1441 gtagtaaaca tcttcaggac ttttgttgca tcctcagcat cttcatatac tacagcgtta
     1501 ccccctttgc caataacatt accaggcaca ggcaagctgt tatagtccac actcggtaac
     1561 tctggcggtg cataatcaac aggaggtaaa tcaggtctat tcgaatgaac agcacctctt
     1621 tccatagccc tattcactgg cgatgaattc agcatcacct caattttcct gctaatctct
     1681 cccttaggcc atcccagcct gtgcaacaga ttagtaaagc aaccactatg actttctctt
     1741 gtaacgcaaa acttattatc agtgacaaca acacgatatg ttctgttgcc cacctttact
     1801 tgcgccccat tatccgagct agcagctgca tcccttacag aggataaaat acgactgtca
     1861 ggagaagtca ggtttctggt taaagaattc catgaacacc ccaaatttac agaagatggt
     1921 gatagcatac atttcaacct tcaaaatgaa tcaatcttta ctttcttaac aaacatcacc
     1981 atgacatgac aacaaaaacc ggagccggac tccggttttg tgaagctgtc gggttacttc
     2041 atcccgccaa tattttccca cgtcccgtca gcacgcagga tttgcagcgg tcttaccaca
     2101 cactgtatca gctttttatc tgtatccagt atcaccacct gtgtgattac cctgtcctgc
     2161 tccggaataa tgccattctc atctgactcc aggatgtctg ccggccccag tcgcagttgt
     2221 gctgtaagcg actgcacgtg ttcacggcca tcatgctttc cgcaaccaca cagacgctgc
     2281 ataagttttt ttagtatatt catgtcattc tcctgttctg cctgtatcac tgcccacttc
     2341 atccagcccc ttgacatcct gccacggccc gtcaccaaac ctgacctgca aatgctgaaa
     2401 aaaaccctga acccgtgtgg catctttggg gtcaagaaag gtcagtccgg tgatgagtgc
     2461 gccatctgta tccgggaacc agccattgct gtttgtctca ataatgtttc ccggccccag
     2521 acggaaccg
//
LOCUS       DAZFKM010000305         2526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFKM010000305 DAZFKM010000000
VERSION     DAZFKM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(201..358)
                     /locus_tag="ACHKAY_005375"
                     /pseudo
     CDS             complement(201..358)
                     /locus_tag="ACHKAY_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            687..1337
                     /gene="nleG7"
                     /locus_tag="ACHKAY_005376"
     CDS             687..1337
                     /gene="nleG7"
                     /locus_tag="ACHKAY_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO5703827.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQKQHCK"
     gene            complement(1562..2437)
                     /locus_tag="ACHKAY_005377"
     CDS             complement(1562..2437)
                     /locus_tag="ACHKAY_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO5703828.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT          757 a          527 c          434 g          808 t
ORIGIN      
        1 accatttttt tcgagttttt tagagaaatt ttcgggaata tttcaggtca atccatgcat
       61 acacaagcaa ttcctgtatt gaataattcc gtagcaatta tgtaaaatca tctccggctg
      121 attttcattc aaactcgcgc tatcgaacgt ccatcagcca gccgtggcac gttcttgcat
      181 acgacgtgcc gcggatccta ttattctagt atgaaatccc taaccgattt caaattctcc
      241 agactgctca gattctcttt tatcccacgc tggcgctgat aaatctcgtc attgcaatcg
      301 acctgcgcta tcgctgccgc agttcttcca gtttctgcat cgataactcc atcttctgag
      361 caataaacct cacctatgca atattttatg tcaattattt caattgcatt aatatcttga
      421 atggattaca tagagttaat gtatccagta ctccctattc tcttgcatat aatacatgtt
      481 gcaacttaca tctcagcgct atgaaaaaac accacctctc tctttatgaa attctggatt
      541 taccaagcgc taatttatca tttcaatcta cctttaagca ttgcatttat ctccctacga
      601 ggtcatactt taggaagtta aatatgaatg ataatatacc tacagcgcga aatcacaaac
      661 aatcgacttg tattacagaa aaaaacatgc ctatcttttt aaacttcaca gcaggcagta
      721 tcttacctga gaatgagcta gcatctttac gttatattgt gcagcaaaat caaaatgata
      781 ctgtaatcat aaaagaacgt tataaaatgg atatccgtta tatcgaatca gtcaatggtt
      841 ttacagtaaa tcctgtatgc agtaatcatt tctccatatt tatggcgaga caaaacacta
      901 ttgctcgcaa cctggaacag cagatcaaca acggacgaag ttttgcacaa atatctcagg
      961 attttatgct tcaattatct tcaaatatag gatggaaaaa aggggccgaa aacgccctta
     1021 aaaataaaat ccattctcat tcatttgttg taaatcctga tgaattctct tgcgacacac
     1081 aatttcttaa gtgcccaata acattatgcg ttccagaaaa aggggttttt gtcaagaacg
     1141 cactgaactc caacatatgc actctttatg ataagtctgc gttcatgaat ctcacaagag
     1201 aacatctacc ccaccctctc agcagggaaa agatagtaaa agaaatgatt attgaaagga
     1261 atatgtgtta ttttgacacc ataagtcagc atttcataat tatggatgca gaccaacaga
     1321 aacagcattg taaataaaat gtaataatta catactatta gtgattctca tgcatcgtaa
     1381 gcggctcgcc agaaccgtat tgatatttac tgagcgctca gatcaacttt ccatggcaac
     1441 agatcgcgta cccggtttgc cggccagtcc tggatatgct cccgccgaca atattaatgg
     1501 ccaggaaatg tttaaaacaa tgattctgtt tcctaaccat caagtgtata tgctggctca
     1561 attactcaat atctatgggt tgagttctac ccaaagtggc tctgtagtcg cttcttctta
     1621 cagtatgttc aggtgttgca ggcacagttg tggagcgtag atgcgttgtt ggtttaccat
     1681 gctctggtac ataaaaaacg ccagtgccat ggtaacctga tatagaaacg ttaattccgc
     1741 gacgttcaaa ctcggcatag acgtgttctg ctaaagacct cttttgacca aataatgccc
     1801 tggccaacca gccgttattc atatttgcgg atttttctat ttcatcagga gagaagtctt
     1861 tgttttttat tatgttggct gagttacagg atgttaatct gatatcatct atttcatgaa
     1921 gattatgttg aacaatgcgg tcgacaatat ctgacggtga taaaaattca tctccgcact
     1981 taagaagagg tagaccagca gaaccatgcc cagacagata aattttgttg tattttcctg
     2041 gtgttaaatc atctggcagg atggttttca tctctgtcgc tgtaatactt atagctgcag
     2101 cagcaacaac atcgctatta cttgattgta gatggctttt atttgctcca ggatatgtaa
     2161 actccatttt ccttttatca aaatcttgtt ttgtagcatc attcaggaat aataaatcat
     2221 acggtttttt ccccgttgtg actcttgaaa cattttggcc aagaatatta agagtatagt
     2281 cattaacgga tttcattcct aaaaaaacaa gtaatccttc tttctcttgc gctttttcga
     2341 ttgttctccc tgctaaagtt attgggatgg attgagattg ttttgtcgga actgatacgc
     2401 tggcgctaat aggtagtagg gttcttatgc taaacataca acctctttct taattttcga
     2461 acctgtttag gattctgtgt aaattcaaaa taaacctgtc tgaacattcc taacaaacat
     2521 ccaccg
//
LOCUS       DAZFKM010000306         2522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFKM010000306 DAZFKM010000000
VERSION     DAZFKM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(151..2418)
                     /gene="adiA"
                     /locus_tag="ACHKAY_005378"
     CDS             complement(151..2418)
                     /gene="adiA"
                     /locus_tag="ACHKAY_005378"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO5703829.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          583 a          617 c          687 g          635 t
ORIGIN      
        1 acaggcaaaa aaaagcgcgg ggtggggccg cgcaaaaagt atgacatttt tgtttgcagc
       61 aatttttatg aaaacgttgc catactcaaa aggcctaagg ccaggcggcg taatgttatt
      121 taaacaatta cgccttcagc ggaatagtgg ttacgctttc acgcacataa cgtggtaaat
      181 accgtcaata atttcagtcc cttcagtttc gtgttcaaat ccagggaaat ggtggtccca
      241 ggattgcagc gagcgtaaat aacttacttg cggactgttt ttatcgccga agttttcacc
      301 agacagcagc atcgggattc ctggcggata cgggataact gagtttgccg cgatgcgtcc
      361 tggcagattt tcaatggata ccagttcgac attgttgtcg acaatcgcgt tgtacgcttc
      421 acgcggggtg atttccgcca tcggcaggcc ggaataggct tcgttcaacc gtgcgccagg
      481 gttgttttct ttcagccagg caaacatggt gtcacccaga tcgtgaatcc ccatgttcgc
      541 gtaagtgtca ggatattgtt caacaagttc cggcatcacc tgcgccagcg gtgtgttggc
      601 gtcatagtgg cgtttgaagg aacaaagggt gttaaccaga gtcccccatt tcccacgggt
      661 tacgcccata gagaacagga acataatttg gaagtcagtg gtgcgggtag gcacaatgcc
      721 gtggcgacca agccaggcag tgaccagcgc cgccggaaca ccggtttctt ccagttcacc
      781 atcttcaccc attcccggag caaggatgct gactttaatt gggtcgagca tactccagtt
      841 atccggaata tctttgaagc cgtgccagct ttcgcccgga tgcattaccc agcagtcctg
      901 aacggtggtc agcagtttgg ttggtgcgtc agcaaagtca taggttttgc cggtttgtgg
      961 gtcggtgacg acttctttgt tccacggttt gaagaaccag ctaccgtcag cggtgaactc
     1021 tttatatagc cgcgccatcg cctgacggaa atcaaccgct tcgtcaatca cttcctgtgt
     1081 cagtgacagg ccgctgttgc cgtccatcat cgacaccgcc acgtcgttgg atgcgcagat
     1141 ggcatacagc ggggaggtgg tggcatgcat catgtaggcc tggttgaagc gggagaagtt
     1201 aatcgcccca cgaccttcac gtacatgaat ataagaagcc tgtgacagcg cattcagcag
     1261 tttgtgggtg gagtgggtgg cgaaaacggt aggaccgttg tgatcgccag gttcgccgcg
     1321 catggcatag tgatcggcat agatcgggtt gaaacgtgca tagccgtacc aggcttcgtc
     1381 aaagtgcaga cgatcggagg ttttttccag cagatcctgc gcttctttag cgttataaca
     1441 cacgccgtca taggtgcagt tggtcaccac gcagtaagac ggtttttgcc cggctttgtc
     1501 tttggtcagc gggctttcac tgattttctt ctgcaaggtt tcaggttgca tttcctgcgg
     1561 atagattggc ccgataatgc cgtagcggtt gcggcttggc accatataga ccggtttcgc
     1621 gcctgtcagc atcaagcctt gttcgatgga tttatggcaa ttacggtcaa cgaccacgac
     1681 atcgttatcg gtcatgcaag cctgcatgat ggtgcggtta gagccggaag taccgacgac
     1741 taccgaccag gagcgatcgg caccaaatac gcgtgcggca tatttttcgc tttcgccaaa
     1801 tgcgccagta tggtcaagca aagaaccgag ggaagttcgt tcgatgccca tgtcggtgcg
     1861 gaacagattt tcaccatagt agtcatggta gaaacgtccg gcgggtgttt tggtaaaacc
     1921 aacgccgccc tggtggcctg gcgctgccca ggaatattca tggatgtcac tatatttcat
     1981 cagcgcgctg aacagtggcg gcaacagctg ctggcggtag cgggtcatcg cggcaacggc
     2041 gcgtccggcg ataaagtcgg cggtatcttc cagaatccag gcgaattcat cgacaagctc
     2101 cagcagatcc cggtccattg cggcgagggc tttttcccga tcgcccaaca ggaagaccgg
     2161 cacgttttgt tggcgctcat gaagcttacc gatcaattgt ctgacgtttt gatgttcgtc
     2221 cggatgttcc atttgatagc tgaacatcag gcagtcaatg gcttcgtttg aagagagaat
     2281 ggcaaaacca tcatcaaagg aggtggattt aatcacggta acattttgct ggcttaaagc
     2341 atctgccaga cgctcaacgg cgttaccgac ccaggtgtct tgatggagaa actcgctttc
     2401 aacaattaat actttcatca ttgcttaccc ggttatgaag gaatcttcgt tgcgggcaag
     2461 tatcttccgg ccttttcggg ctgtaaagcg cgtgaaaaac aaacattttt gaaatattta
     2521 gt
//
LOCUS       DAZFKM010000307         2491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFKM010000307 DAZFKM010000000
VERSION     DAZFKM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..369
                     /locus_tag="ACHKAY_005379"
     CDS             <1..369
                     /locus_tag="ACHKAY_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703830.1"
                     /translation="QKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNIIN
                     TDLVIGEKIHFRTLSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYI
                     SKIKDNHNTPWLLNNENVQE"
     gene            complement(1240..1380)
                     /locus_tag="ACHKAY_005380"
     CDS             complement(1240..1380)
                     /locus_tag="ACHKAY_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5703831.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1682..1945)
                     /locus_tag="ACHKAY_005381"
     CDS             complement(1682..1945)
                     /locus_tag="ACHKAY_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO5703832.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2368..>2491
                     /locus_tag="ACHKAY_005382"
     CDS             2368..>2491
                     /locus_tag="ACHKAY_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO5703833.1"
                     /translation="MKTKKVMMIALVSSALALSGCGAMGTAIKKRNLEVKTQMSE"
BASE COUNT          786 a          572 c          447 g          686 t
ORIGIN      
        1 caaaaaaaac taaaacagac gtctgtgcct gattttattt attattcaat agaaaaacac
       61 aacaaatatc ccatatatgt attacacatg ccatcaaaca atataattga aataggctac
      121 aatataataa acaccgatct ggtcattggc gaaaaaattc atttcaggac actaagcaat
      181 cgttctttat attttaacct cacccaaccc ccattaatcg ccacaataaa agatgatgtg
      241 ttttgcactc ttcatgatta ctataaccac aataatgaaa cgaaatccac tattgacaat
      301 tacatatcaa aaataaagga taatcataat accccatggt tattaaacaa cgaaaatgtt
      361 caggaataaa aaaaagagac aattcccgag ataaaacaaa agagcataaa catatgaacc
      421 ggaaattcta tttcaaaaat atcctgtggg gaatgtgccc cattataata tccatattgc
      481 atctgatata tgacacacca acagaagcat cgataaaagt tgacttattt ctgatactca
      541 gtggcctggc ccttccttgt tcaatatacc tgacaaaaac aatcacacta aaatacacat
      601 ccatatcatt ctggaaaaaa cctcttttct ggtcttatgg tatatatcat tttcatcttg
      661 tttatataat gacatgtata gttttagcaa taccttctgc aattatcgtt ttattctttc
      721 acttttatca aaacatcacc agaaactgga agtgacaacc gcaataatga acataaaaag
      781 gcaaatcaga atggcagtaa atcaataaag taccatttca tatgaatcat cagaaaaacg
      841 gtggacgcga catccagcac gaggcaaaac acagcaacaa aataagccgg atatcgcgcc
      901 ctgtactttt ctaactcaac tcccggcatt gtgcaagttt taatcgcagg tggattcaga
      961 gccacgcccc cattgattca gcctgaagac acgtatatcc ttggagaaag caggaatctc
     1021 attgctgtga aattccttac agaatcttca gccccatttt atggggcttt ttttggtgcc
     1081 cccacgctgt taccacactt tcgggatgcg aataaaaaag cccacagttt ttacactgcg
     1141 gacttttgtt ttttctttgt gatttgtgtg caagggtgcg actttcgacc gaacctttgg
     1201 caaccggata gcggggactg ttgtccccgc tgcggtttct tacggtttac actgcaagaa
     1261 cgccgcaatt tccgaccaca tgaagcttac cctgaattca cacggtgatg cgtgtaacac
     1321 ccagacggtc agcaacatgg tcaggcataa catgcccaca atgatcgttt tttgcggcat
     1381 agcacttgct ccttttacag agaggcgcta accttccact tgctgaaggt agatggtcag
     1441 ggcctcgggt taacgtttaa gttgactcgg ggcctttcca catccggcct tcaggtaatc
     1501 cttccagccg tcagccgaaa ggcacccgcg cgtaatgtac tggttttcag agaggtccgc
     1561 aatcttccgg tgacgagatc atctgcacac gccccggtgg gacgggctaa cggtatattc
     1621 aggtgcagga tctctctgtc tggtgcgttt ttaaattgtc aaacgcaccc tggcggcaaa
     1681 atcagccacc ttttttcgca tcaacaaaac gcccaaaacg ctgggtaaag cgtgcaacat
     1741 taccttctga agcgacagtt ctcagtttac ctgtataaaa cggatgcgac ttagaagaaa
     1801 cgtcaattgt aacatatggg taagttacac catccagttc aattacccgg tcagttttga
     1861 tcgtcgaacc gatcctgaag tactcatcaa tactggtatc gtgaaatact actgtacggt
     1921 attccggatg gatattcggt ttcacaacag ttttccccct ttaagtgaaa attccgcata
     1981 aaggtacata ttcagacggt cagattcaac tgtctgttct tataacctca ctctctttca
     2041 gtattacaat atgttatata attataaact ggacttcccg aaaaaatcca ttaaataaga
     2101 agcgagccgt tgcggaggct tgctctctcc tttaacctca tgtgcaatat ccgtaacgcg
     2161 ctcacataaa tcatcatctg ataatataac aacacccatt attttattct gacctaaaaa
     2221 cttcttatga aataaacgtt tacccgacgt atctgatccc gctgttttta cctgtatcct
     2281 ttaacatact tgagaagccc gctgaaaaat ataccgtaaa ggcggttctc tgtcttccat
     2341 aaacctgtcg gataagaagg gcattaaatg aaaaccaaaa aagttatgat gattgcactg
     2401 gtcagctctg cgctggctct ttcaggatgt ggagccatgg gcacagcgat caaaaaacga
     2461 aatctggaag taaaaaccca gatgagtgaa a
//
LOCUS       DAZFKM010000308         2451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFKM010000308 DAZFKM010000000
VERSION     DAZFKM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..937
                     /locus_tag="ACHKAY_005383"
     CDS             <1..937
                     /locus_tag="ACHKAY_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO5703834.1"
                     /translation="FNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDF
                     LNGDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQV
                     YIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQII
                     NVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKV
                     GNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKE
                     QVNGDSLLINWNCAASKSPPKPPDEKQDTPDMREY"
     gene            1005..>2451
                     /gene="rhsD"
                     /locus_tag="ACHKAY_005384"
     CDS             1005..>2451
                     /gene="rhsD"
                     /locus_tag="ACHKAY_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO5703835.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDT
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVV"
BASE COUNT          542 a          662 c          811 g          436 t
ORIGIN      
        1 gttcaactgg gaccgttata acccggcaga ccaagacagt tcgtgctgga tccgtgtggc
       61 acaggcgtgg gcaggcaccg gttttggcca cctggcgata ccgcgtgtgg gtcaggaggt
      121 gattgtggac ttcctcaacg gcgacccgga ccagccgatt attatggggc gtacctacca
      181 ccatgaaaac cgcacacccg gcagcctgcc ggggacgaag acgcagatga cgatccgctc
      241 gaaaacctat aagggcagcg ggtttaatga actgaagttt gacgatgcga cagggaaaga
      301 acaggtctac atccacgcgc agaagaacat gaacaccgag gtgctgaata accgcaccac
      361 ggatgtgata aacaaccatg ctgagaccat tggcaacaat cagatgattg cggttaccaa
      421 caatcagata cagacggtgg gcgttaacca gatagagacg gtgggcagta accagatcat
      481 taacgtgggt tctgttcagg tggaaacgat tggacttgtt cgtgcgctga ccgtgggcgt
      541 ggcgtaccag acgacggtag gtggcattat gaacacttcg gtggctctga tgcagtcctc
      601 gcagatcggt ttgcataaat cgttgagggt cgggctgggt tatgacgtca aagtcggaaa
      661 taacgttacc ttcaccgttg gtaaaacgaa aaaggatgat accgggcaga ccgcgattta
      721 ctccgccggt gagcatctgg agctctgctg tggtaaggca aggctggtgc tgacgaagga
      781 cggacaaatt tttctcaacg gcacaaaaat tcatttgcag ggtaaggagc aggttaatgg
      841 tgactcactg ttgattaact ggaactgtgc agcctcgaaa tccccaccga agccccctga
      901 tgaaaagcag gatacgccgg atatgagaga gtactgatcc cgtacctgtt cggaattcag
      961 taacaaacca gcattaaaaa taaacagaag aaggaatttg aatcatgagc ggaaaaccag
     1021 cggcgcgtca gggagatatg actcagtatg gcggtcccat tgtccagggt tcggcaggtg
     1081 taagaattgg cgcgcccacc ggcgtggcgt gctcggtgtg tccgggcggg atgacttcgg
     1141 gcaacccggt aaatccgctg ctgggggcga aggtgctgcc cggcgagacg gaccttgcgc
     1201 tgcccggccc gctgccgttc attctctccc gcacctacag cagctaccgg acgaagacgc
     1261 ctgcaccggt gggcgttttc ggccccggct ggaaagcgcc ttctgatacc cgcttacagc
     1321 tacgtgatga cggactgata ctcaacgaca acggcgggcg gagcattcac tttgagccgc
     1381 tgctgccggg ggaggcggtg tacagccgca gtgagtcaat gtggctggtg cgcggtggta
     1441 aggcagcaca gccggacggc catacgctgg cgcggctgtg gggggcgctg ccgccggata
     1501 tccggttaag cccgcatctt tacctggcga ccaacagcgc acaggggccg tggtggatac
     1561 tcggctggtc agagcgggtg ccgggtgctg aggacgtgct gccagcgccg ctgccgccgt
     1621 accgggagct gacggggctg gcggaccgct tcgggcggac gctgacgtac cggcgtgagg
     1681 ccgccggtga cctgaccggg gaaatcaccg gcgtgacgga cggtgccggg cgggagttcc
     1741 gtctggtgct gaccacgcag gcgcagcgtg cggaagaggc ccgcacctct tcgctatctt
     1801 cttctgacag ttcccgccct ctctcagcct cagcgttccc cgacacactg cccggtaccg
     1861 aatacggccc cgacaggggt atccgccttt cggcggtgtg gctgatgcac gacccggcat
     1921 acccggagag cctgcccgct gcgccactgg tgcggtacac atatacggaa gccggtgaac
     1981 tgctggcggt atatgaccgc agcaatacgc aggtgcgcgc tttcacgtat gacgcgcagc
     2041 acccgggccg gatggtggcg caccgttatg caggcaggcc ggagatgcgc taccgctacg
     2101 acgatgcggg gcgggtggtg gagcaactta acccggcagg cctgagttac cgctaccagt
     2161 atgagcagga tcgcatcacc gtcacggaca gcctgaaccg gcgtgaggtg ctgcatacag
     2221 aaggcggggc cgggctgaag cgggtggtga aaaaagaact ggcggacggc agcgtcacgc
     2281 acagcgggta tgacgcggca ggaaggctca cggcgcagac ggacgcggcg ggacggcgga
     2341 cagagtacgg tctgaatgtg gtgtcaggcg atatcacgga catcaccacg ccggacgggc
     2401 gggagacgaa attttactat aacgacggga accagctgac ggcggtggtg t
//
LOCUS       DAZFKM010000309         2426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFKM010000309 DAZFKM010000000
VERSION     DAZFKM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            399..1406
                     /locus_tag="ACHKAY_005385"
                     /pseudo
     CDS             399..1406
                     /locus_tag="ACHKAY_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2026..>2426
                     /locus_tag="ACHKAY_005386"
     CDS             2026..>2426
                     /locus_tag="ACHKAY_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFO5703836.1"
                     /translation="MGNAMHSLKTLLQLPCGWRCSRQIINSDGIPLHLRGKRKTAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGQTLWLVFSVRHRYSRNPACSRKIFAESFVPFGGS
                     HLQSSQALQNLQRQLGLIAGGEAGKRAATAVG"
BASE COUNT          633 a          549 c          633 g          611 t
ORIGIN      
        1 aggcagccta gcggctgcgg cttgtcatgg cctgaaatta ccgttataaa aacaaacaat
       61 gtcattgtct ttcaggtagt tatatggcac gttcagctaa accccgtaaa cgaaaactcg
      121 cacctcaaag aagcaaactt ccccgctatg ttgtaaaact tcacgaagat gatttttttg
      181 acgaagaaga agtagagatt ctgcgcttcg ataattttga cgatactgtt gagtgttgcg
      241 cagacctgaa tattccgttc tttgtggatg ccggaaacaa aaagctggtc ttctggtttg
      301 tacgtgttga tgacgaaggg catccggaaa tcgcccgctg tacagagcgg gagtttacga
      361 ccattcttgc cggtatcagc gccggcggca tgtactgccc ggactgttgc aatacaaaga
      421 aggtctgttt tcggtgtaaa aaacgctcct gcccacactg cggggtgaaa gcgggtgcac
      481 agtggataca gtatctgctg agtctggtcc ccgacggccc gtggcagcaa ttgtattcac
      541 gcttccctgc cagtactggt ccctggtgtt ccacaaccgg tggttactgg cagagatgag
      601 ccgcatagca gcagatgtga agccggggat attcacggta atccacacat ggggccggga
      661 ccagcagtgt catccacata tccacatatc catttatcca tttatcgaca actgccggcg
      721 gtgtgacatc acatcacacc tggaaaaacc ttcattttta cgcccgtaag gtgatgagta
      781 tgtagcgata ccggataaca cggttactgt cacggaaata cccggaacta ttgataccgg
      841 atgagctggc tgctgaagga agcagtaaac gggaatggaa tcgtttcctg gacagtcatt
      901 accgacgggg caggaatgtc aacgtatcaa aggtgatgga taacgccaca catgtggcgg
      961 tgtactttgg ctcttacctg aaaaagccgc cggtgccaat gagccgtctg gagcactatg
     1021 ccggtcagga tgaaatcgga ctgcgttaca acagtcaccg cacacgacgt gaagaatacc
     1081 tattgatgag tggtgatgcg tttatggaaa ggttctcctg gcatgtggcg gataaggggt
     1141 tccgtatggt gaggttctac gggttcctta gtccggcgaa acgccggttg ctggaagaat
     1201 tagtgtacgt aataacagag acggtgataa aaacggcgat gcaaatcagg tggagaggga
     1261 tgtatcagcg gttactgaag gttgacccgc tgaaatgcat cctgtgcgaa agtcagatgc
     1321 ggtttacggg gctgaagcgg ggttaccgtc tggcagagct ggttatgatg catgagccgc
     1381 tggcccgaat gcagtattgc ggctgagagc cgcagagggg aagttgcgtc cattttgagg
     1441 gaaatggagc aaaaaaacgt caccgatacc ctgtatcaat caatgtcagc aatataagcg
     1501 ctgatatata cccgatgagg ttccggatgt gttgaataaa tgttcgtttt ttgttttctt
     1561 aatctttccg ggggataaat tcctgtcgat gaacatatca aaatcaggaa attacacgaa
     1621 tctatgtaca ggctctctgg gaacacttta cgctttgaca cagagggcta aataatgagc
     1681 gattcagatg ccatttttgc atacagagga ttgacagcat tcccatgcta attgttaagg
     1741 tttaaattga agtatatcta tctgtcattt ttgcaaatga catgcggtag cataacgttc
     1801 cgaggtctct tatgcagaca tacgtggcga cattaaccac tcccagatac agccagcctt
     1861 catccgtacg taagtacgtg atgtctcgcg cccacttctg gctctggcta ctggcgtaaa
     1921 aatcctgctc ctgtcacgaa cggtgcaaaa gtggttcttc cgcaacctgg gtggagaaaa
     1981 tggatgaaac tttatggtga acaaggatct aagctgtttt tagttatggg taacgcaatg
     2041 cactctctta aaacacttct acagttacct tgcggatggc gatgtagtcg acaaattatt
     2101 aactctgacg gtatacccct ccatctccgc ggaaaacgca aaacagcaca atgtcctgaa
     2161 tgctttaagc gtagcgactc tgttcatagt tgtcgtcggc gccggataca gcatctaccc
     2221 tgctcagggc agacgctatg gcttgtattt tccgtccgcc acaggtacag ccgtaacccg
     2281 gcttgttcac gaaaaatttt tgccgagtcg tttgttccct tcggtggttc acatctgcag
     2341 tcttcacagg cgttacaaaa tttacagcgt caactgggat taatcgccgg aggtgaggcc
     2401 ggaaaacggg cagcaacggc agtggg
//
LOCUS       DAZFKM010000310         2398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFKM010000310 DAZFKM010000000
VERSION     DAZFKM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            14..820
                     /gene="nirC"
                     /locus_tag="ACHKAY_005387"
     CDS             14..820
                     /gene="nirC"
                     /locus_tag="ACHKAY_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO5703837.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            839..2212
                     /gene="cysG"
                     /locus_tag="ACHKAY_005388"
     CDS             839..2212
                     /gene="cysG"
                     /locus_tag="ACHKAY_005388"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO5703838.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          538 a          601 c          665 g          594 t
ORIGIN      
        1 aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg ctgcacgcat
       61 tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca tggcgggcgc
      121 atatgtgggt cttgggatca tcctgatttt cacgctcagt aatttgctcg acccgtccgt
      181 acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga ttatcgccgg
      241 ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag cgggcagcat
      301 cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc tggtcggttc
      361 cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg tagataccag
      421 catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac tgttcttcaa
      481 aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc gcactgaagg
      541 ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt ccggctacga
      601 gcactctatc gcaaacatga cgctgttcgc gctctcctgg ttcggcaacc acagcgaagc
      661 ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta atactttatc
      721 aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga atcgtccggt
      781 tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg gtttttacgt
      841 ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg tcggcggtgg
      901 tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct taacggtgaa
      961 tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt taaccctcgt
     1021 cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg cagcgacgga
     1081 tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca tcttctgtaa
     1141 cgtagtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg accgctcacc
     1201 gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc tgttgcgcga
     1261 aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg ccgggcaatt
     1321 acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt tctgggagaa
     1381 attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga aagccattac
     1441 tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag tggtgctggt
     1501 tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac aaattcagca
     1561 ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc tggtacgccg
     1621 cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac cgcaggaaga
     1681 gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc ggctgaaagg
     1741 tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt gcaacgcggg
     1801 tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg cctattcggg
     1861 tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg gacacttaaa
     1921 aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc tggtgttcta
     1981 tatggggctg aatcaggctg cgactattca gcaaaagctg attgaacacg gtatgcctgg
     2041 cgaaatgccg gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg tgattgacgg
     2101 tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc taattattat
     2161 tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt aatttaaccc
     2221 gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa ataaaacata
     2281 gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg acttacaaaa
     2341 ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta acgagcag
//
LOCUS       DAZFKM010000311         2370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFKM010000311 DAZFKM010000000
VERSION     DAZFKM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(85..297)
                     /locus_tag="ACHKAY_005389"
                     /pseudo
     CDS             complement(85..297)
                     /locus_tag="ACHKAY_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            447..1115
                     /locus_tag="ACHKAY_005390"
     CDS             447..1115
                     /locus_tag="ACHKAY_005390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO5703839.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1060..1197)
                     /locus_tag="ACHKAY_005391"
     CDS             complement(1060..1197)
                     /locus_tag="ACHKAY_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO5703840.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1170..1754)
                     /locus_tag="ACHKAY_005392"
     CDS             complement(1170..1754)
                     /locus_tag="ACHKAY_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5703841.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1754..>2370)
                     /locus_tag="ACHKAY_005393"
     CDS             complement(1754..>2370)
                     /locus_tag="ACHKAY_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5703842.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHSHVDGRRFN
                     TSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMANTNSAGAHTHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          627 a          569 c          511 g          663 t
ORIGIN      
        1 acggtccttt tttcgccgaa aactgatatt actttaatca aaagttgacg tgccagattc
       61 gaagtaaatt tataaaatat caaatcaata tacattttgc accttcgaga tagtaatgac
      121 tgttactgta acatgagtta caaacagctt catacccaga gctactgatg tatcaactcg
      181 attcaacaat acccgatatt tattccatgc ctccagcaac gatctttctt cctccgttgc
      241 gatttccaga tctacagcat cctgcagtgg cgcaatatac tcactgaatt cctggatgta
      301 gaactgtgtg gtgacggtct tccagccatt cggctcctgt tgtatcgaag cataccaggc
      361 tatttcaata tcgctatgct gcggcagcat ttaacccctt gtaattcatc gccataattg
      421 atttaattca caaataaaac tataacatgg tgaaattaat gaaaaaaaac acagatgatg
      481 gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt ttgggagtat
      541 caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac tttctggaac
      601 atttaggtaa caaccatctg gatattggtg ttggaactgg gttttacctt actcacgtac
      661 ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac gcggcatcta
      721 caagggctgg ggaatcaaaa attaaacata aaattagcca tgatgttttt gaaccttatc
      781 ccgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt cactgcctgc
      841 ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg
      901 atggaactct atacggagcc acaattcttg gcgatggagt tgtgcacaat agcttcggtc
      961 aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa gattccgaag
     1021 aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag gttcaaggta
     1081 ctgtagtaat gttttccgct tcagggaaaa aatagcatcc aaccgcagca cgttcttgct
     1141 taagacgtgc tgcggcataa tcccaatgat tactccctga cagggttcgt aggccactca
     1201 atatcaggtg cagttgatgt atcaacacgg ttcagcaaca cccgatactt tttccaggct
     1261 tccagcaatg aggtttcttc ctccgttgcg atttccagat ctacagcatc ctgaagtggc
     1321 gcaatatgct cactggctac ctgcatcagg ctgtttttta tttcttccgc ctctcggatc
     1381 cggaacagtt tttctgcttc tgcatctttc acccaggctg tgccgttcca cttctgaaac
     1441 tccccttccg gcgataacca ggtaacattt tccggtaacg gaccgagttc agaaataaat
     1501 aacgcgtccc ctgacgctac gtcataaacc gttttacccc gatggtcttc aacgagatgc
     1561 cacgatgcct catcactgtt gaaaacagcc acaaagccag caggaatatc tggtggtgca
     1621 atatcggtac tgtttgctgg cagacctgta tgaggcggaa tatatgcatc accttcacca
     1681 ataaattcat tagttccggc cagcagatta taaattttta tggtccgtgc ttgttcactc
     1741 attctgaatg ccattatgca agcctcacaa tatagttaaa tgcgatgttt ttgacggtgt
     1801 tttccgcgtt accagcagcg ttaacggtga tggtgtgtcc atgtgagcca atcgcaacgg
     1861 agtgcgtatg agcaccaata ccgacagtat gtgcgtgtgc acctgcgctt gcagcagtgc
     1921 cggacagtga gtgggtatgc gcgccagcag agttcgtatt cgccatcgtg ccaatgccca
     1981 gaccaaccgc cccttgtacc tggtatgagt tacttccgac agctgtagaa ccatactgat
     2041 aggtgtcttt aaaagtgctt gtattaaacc tgcgaccatc aacgtgcgag tgtgctccgg
     2101 ccgagcttgt agagccgctc agactgtgcg tatgagcccc ggtgttattc gtggatttag
     2161 tgccgtaatc aaacgacgat gtggttttcg tccccaaatc cgtactggat gcgctggcgc
     2221 tgtgggtgtg cgatttaatg ccgtcctgtt cctgagacaa tacggcacga ccactggcgg
     2281 gtttgccctt aatcgtccag ccacgcatat cagggatcac gcctgacgga taagcggctg
     2341 caagtttcgg gtatgcagat ttgtcaaaag
//
LOCUS       DAZFKM010000312         2370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFKM010000312 DAZFKM010000000
VERSION     DAZFKM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..296)
                     /locus_tag="ACHKAY_005394"
     CDS             complement(<1..296)
                     /locus_tag="ACHKAY_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703843.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAG"
     gene            451..1287
                     /locus_tag="ACHKAY_005395"
     CDS             451..1287
                     /locus_tag="ACHKAY_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO5703844.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDSQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            1311..2363
                     /locus_tag="ACHKAY_005396"
     CDS             1311..2363
                     /locus_tag="ACHKAY_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO5703845.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
BASE COUNT          590 a          658 c          618 g          504 t
ORIGIN      
        1 cctgcggcca tcacatccgg ctggccatca tgcagctttt taagctgccg ggtcagcagg
       61 tctatttcct taaagtcacc gcctgtttta ttctgttttt cagtaagctg gatgaggcgc
      121 gcatcgatgg actgcgtgac acgctgcacg ggtggcgttt catcccactg gtcacgtttt
      181 ttccacgcat aaatcgtgtt cgggtttatt cccatcagac gtgatatttc tgcgggcgga
      241 taaccctgcc agtaaagttg ccgcgcacgc tggcgcacaa aagcgtcctg aatcattgct
      301 ccccctgagt aattacagga agattacccg cgcgcgaaac tgttctcctt aaccccctgt
      361 tctggccgtt ttcttacaac aaaaaccctt tgtatcagcc tgttacgctt tgccatcatg
      421 actgaagaac cagtcagagg ggcaaaaact atggctaatg aaaaaaagac atcccgcaaa
      481 aagtttcgcg tggctgtctc cggatcaact gttgatggcc gtgaaatcag tccggtgcat
      541 ctgcgtgaag ccgccgagaa cttcaacccg gatgtttacg ctgcccgcgt gaacgttgag
      601 cactatctct cgccatgccc gtcaagcgaa ttttccgcaa tgggcgatgt caccgcactg
      661 agtacggaag acattacgga aggtccgctg gccggacgta ctgcgctgta tgcagaaatc
      721 gaaccgaccg agcgcatgaa gcagcttgtc gcggacggca agaaaatcta ttccagtatc
      781 gaactgcacc cgcagttctc cgttaacggg cgcgcctatc tggtcgggct ggcgatgacc
      841 gacaccccgg caagcctggg cactgagcgc ctgaaattca cggcacagca acgtcaggcg
      901 gtgatgacgt tcaacagtat ccagggtgaa gcgccgctta tctccgaagc catcgagtct
      961 gaaatcatcg aaatggcaga acaacgccag gaagaaggca cccagtggtt taaccgcgta
     1021 atggggatta ttggccgtgg ccgcaaagcg gatgacgcca gtttttcccg tattcaggaa
     1081 gcggtggaag gcgtcgcaac gtcacaggcc gacattatcg accgttttaa tgtgctggaa
     1141 acccgccatc agcaggacag ccagaaaatt acgtcactga ccacagagct ggcagcactg
     1201 aaggaaaaac tgcgcacgca ggacggcgat ccgcagaacc ggttcaccgc aacaggtgca
     1261 gcctccgacc agctggctga cttctgataa gacaaaggag caaatttttt atgaatctgg
     1321 tgatgtcaga tattacccgc aacaagctgg gttgctatat ggcgcagcag gcgtcgctta
     1381 ataatattcc ggtttccgca ctggtatcgc gatttaccgt ggaaccctcg gtgcagcagc
     1441 gttttgaaaa cgcctcaaag gaaagcaccg aatttacaaa aagaattaac gtgatcggcg
     1501 tgaccgacca gaaaggcgaa aaaatcctcc tggacaccac cgggccaatt gcacgcacga
     1561 ataccagtta tgacggcaca aaacgccgta acccgaataa cgtggttgat ctgaaaaacc
     1621 gcaaatacca gtgcgaacag gtgaactacg acacgtttat ttcatatccg cagcttgatg
     1681 cctgggcggc acatcctgat tttcagtccc gcatcagcgc acagattgcc cgacaggtgg
     1741 cgcttgaccg catcatgatc ggtttcaacg gcacgtctca cgcggatgag tccaacttca
     1801 gcaccaacaa gctgcttcag gacgttaacg tgggatggct ggagcacatc agaaccgacg
     1861 ccagcgaacg cgttatgaat gacgtgacgc tgacctcccg caacatggac aacaccgtgg
     1921 cgcacgcggg taagtatgcg aacgctgatg cactggtaca ggacgcgcgt tcatccctgc
     1981 tggatgaatg gcacaaggaa gctgacgacc ttgtggtgat tatggggcgc aacctgttta
     2041 actcgctgcg tctgcccgtg ctgaacagca tcagcggcca gaatcccaat gcggaattac
     2101 ttgccgggca gctcatcctg tcatcgcgca ccattggcgg gctgggcgtg ttccttgcgc
     2161 cgttcttccc ggatgcaacg atgctgatca cctcgttcaa caacctgtcg atttactggc
     2221 agaaaggttc aatgcgtcgc ctgatgaaag acgagccgga atacaaccgc atcgccacct
     2281 accagtccat caatgacgct tatgtcgttg aagactatgg caagtgcgcg atggtcactg
     2341 gcctgaagtt cgccgacagc taatcaactc
//
LOCUS       DAZFKM010000313         2358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFKM010000313 DAZFKM010000000
VERSION     DAZFKM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.316"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            227..1087
                     /locus_tag="ACHKAY_005397"
     CDS             227..1087
                     /locus_tag="ACHKAY_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO5703846.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1235..1450)
                     /locus_tag="ACHKAY_005398"
                     /pseudo
     CDS             complement(1235..1450)
                     /locus_tag="ACHKAY_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1562..1942)
                     /locus_tag="ACHKAY_005399"
     CDS             complement(1562..1942)
                     /locus_tag="ACHKAY_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFO5703847.1"
                     /translation="MALKPRELSKGNAGAAGLYAFFAEHIDPTCGAVVCDQQFLADHL
                     EVSTRTIRNWLKYLETERALVRIPVAGRVCAYALDPHEVWKGYDNAKEYAAFVTKTLV
                     NKDGDIKRRIMSMFSPEERPDDRE"
BASE COUNT          610 a          549 c          560 g          639 t
ORIGIN      
        1 gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
       61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      241 ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      421 aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg ttgacgccgg
      481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      601 aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag gaccgaagga
      661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
      781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1201 acgtgagttt tcgttccact gagcgtcaga cccctagaaa gccatgctcc agccgcccgc
     1261 attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
     1321 ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct
     1381 cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca
     1441 catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg
     1501 aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
     1561 gtcactccct gtcgtctggg cgttcctctg ggctaaacat cgacatgatc cggcgcttaa
     1621 tatcgccgtc cttgttgacc agcgtcttgg tcacgaaggc cgcgtattct ttggcgttgt
     1681 cgtacccttt ccagacctcg tgggggtcaa gagcgtaggc gcaaacccgc ccagcgactg
     1741 ggattctgac caaggcgcgt tctgtctcaa ggtacttgag ccagttccga atcgtgcggg
     1801 tgctaacctc cagatggtca gccaggaact gctgatcgca gacaacagcg ccacaggtag
     1861 ggtcgatgtg ttcagcgaag aaggcataca ggccagcggc ccccgcattg ccctttgaca
     1921 gctcccgggg tttgagggcc aaccgtacga aaatgtacgg taagaggaaa attatcgtct
     1981 aaaaatcgat tagtagacaa tgccgtccgt taagtgccaa ttttcgatta aaaagacacc
     2041 gttttgatgg cgtttttcta tgtccattat gtcttgatat atcatacagt tacttcacta
     2101 acgtacgttt tcgttccatt ggacttcaga ccccgtgtta cgaacctgaa tagcaaaaaa
     2161 gaatctgttt attattgtaa gtccattggc attgatcgcc ttagttattt atcggtaaaa
     2221 atggcttgtg ataaatattc agctttaggg tgtgataaat gaaacatcta aaataggggt
     2281 cagtttggat atcgaaaaat taggatacat tctttcagaa aggtggcacc tatgaacagg
     2341 tgccaataat taaatctg
//
LOCUS       DAZFKM010000314         2309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFKM010000314 DAZFKM010000000
VERSION     DAZFKM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..371)
                     /locus_tag="ACHKAY_005400"
     CDS             complement(<1..371)
                     /locus_tag="ACHKAY_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703848.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNT"
     gene            complement(633..2240)
                     /locus_tag="ACHKAY_005401"
     CDS             complement(633..2240)
                     /locus_tag="ACHKAY_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO5703849.1"
                     /translation="MKAIDNIFKYVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDITYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELLNTRD
                     MILNELHKHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMESNKDSRI
                     NKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCHYDSERKVLTSHHGI
                     IRDEENACYCLGALQVPFKQMKNPTDPEELANIFNKKHKEHMNDPLLHLIRSNIMKPT
                     AVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQKYGHHGVGVDQNQQFDNGVM
                     GLNSTKKVLDKNNKIIYSSGLSCFQSH"
BASE COUNT          725 a          424 c          362 g          798 t
ORIGIN      
        1 gtattatcaa gatagcagcc gtaatttata ctattaattg tcacaataaa ctgataatta
       61 ttatccctac gtagaatatg agcgcgcatg cccgatagca tactatattt ttctgaatga
      121 ttcgtttgta tggtaaatga aattttgtca tctcgaatag attgttggaa acgttctatt
      181 tgccaatcat caattttttc cacaacttca ccgagcattg tcctggcaat acttgatata
      241 tcatcaccta taccatttgc aatataccca aacagttctt tagtgaagtt agtaacatgc
      301 tcttgcacca tatcatttgt tgcgattgtc gttgtactgt cgttagtgcc tgccacagtt
      361 ccaattttca tcttttacct catataattc accgcgagga atgcatagtt aacatttaat
      421 cttatgacat acacctcatg ttcgatggaa aaataattat attttgcaca aatcattcag
      481 ccttaaagca taatatgaaa tttcttatct catagcagag aaagtcccta aagattgtag
      541 aggcgtcatc agtacatacc tctacaatcg attaagaaaa cctatgaaat actggcagga
      601 ttaaaaaggc ataccaccgt attcatggag ttttaatgag attgaaaaca actaagccct
      661 gaggaataaa ttattttgtt atttttatca agtactttct tcgtggaatt tagccccata
      721 acaccattat caaattgttg attctgatct accccgaccc catgatggcc atatttttgt
      781 ctaatgtata tgctgggatc ttcacatttg agtgtctctc cacatatgaa gatacgttca
      841 ggttttggtc tgaaatcagt agtgttatta atataatcag catatacagc tgtgggtttc
      901 ataatattgc ttcggattag atgtaataga ggatcattca tatgttcttt atgtttttta
      961 ttaaatatat ttgctaattc ctcaggatca gtaggatttt tcatctgttt aaatggaacc
     1021 tgcaaagcac cgaggcaata acatgcattt tcttcatctc ttattattcc gtgatgactg
     1081 gttaaaactt tcctttctga gtcataatga caaacatcga gcgttttaat taagttatac
     1141 gtatcaccgg cagagagttt atgatttgct agtcgtgcag tatagttacc attaaaattg
     1201 atttcatgat ttccagccag aacgacgaca tttttattaa tacggctatc tttattactc
     1261 tccatatttc gcatagaatt tagcaaagta aggatatatt tatcaccaaa aatagtacta
     1321 aatctatcgc ctgtatgatc cccaaggaag atacaagggg ttatcgcatc gtcctttaaa
     1381 aaaacatgtt tgtgcaactc attcaatatc atgtctctcg tatttaataa ctcgtagagc
     1441 actttattat ttttatgctt gtatgcagat gaagccatgt tctcgtgatt taataattca
     1501 cataataaac tccaaccctt ttccctgatg cctatatgcc ctgaaacgag caatgcataa
     1561 agtacagcgc ctactcttcc atctgtatcg ccaaaataag ccgtgttggc attttgtggg
     1621 ttaatcacac cacattgttt ataaaagtct attttagaaa acacagattt aacatagcca
     1681 gcaaacttat ccttaggaaa cgtctcttta gagacatccc aacgccagtc agctttaaaa
     1741 ttaccgctaa gaagagtaaa gaaattttca ggtatgatag aaagcccatt aactttataa
     1801 gttatgtctg atttatctcc agaggaatta ataaaataat aaccgccctt acactcaaca
     1861 gtaacggtaa tgggagacga taattttaac acatggttat tgtctggtat aattgtaaag
     1921 ataaacgtgt cagtagtatt gtcggcgaat ctattatatt cttgaatatc tatagaaggt
     1981 tcactattgt aaaaccatac tttctccatc tgatgactac gatcatttac cctccagtga
     2041 ccatgttgca ggaaatagat gatatgcatg ccaaaatcgt tatctatttg cgaatcatta
     2101 tccagtttat caatttcaac tatctttgaa cgatgtgttt tatcaacctt gctatggtgt
     2161 tgatgattat taactttatc tgaatgctta tctttatggt ttgaagggac gtacttaaat
     2221 atattgtcaa ttgctttcat aaataacctg cgatattttt gacttgttag taacaaaata
     2281 aataatacat gtctgaaaga catattttc
//
LOCUS       DAZFKM010000315         2304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFKM010000315 DAZFKM010000000
VERSION     DAZFKM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..530
                     /gene="ygeG"
                     /locus_tag="ACHKAY_005402"
     CDS             39..530
                     /gene="ygeG"
                     /locus_tag="ACHKAY_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO5703850.1"
                     /translation="MSTETIEIFNNSDEWSNQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            865..2241
                     /gene="ygeH"
                     /locus_tag="ACHKAY_005403"
     CDS             865..2241
                     /gene="ygeH"
                     /locus_tag="ACHKAY_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO5703851.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDILQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          840 a          384 c          384 g          696 t
ORIGIN      
        1 ataatttggc atcacgagac acatcacaga ggaatattat gagcacagaa acaattgaaa
       61 tattcaataa tagtgatgaa tggtcaaatc aactaaaaca cgcattatcg aaaggagaaa
      121 atctggcatt actacatggt ttaactcctg atatccttga tagaatatat gcatatgcat
      181 tcgactacca tgaaaagggt aatataacgg acgcagaaat ttattataaa tttttgtgca
      241 tttatgcgtt cgaaaatcat gagtatctaa aagattttgc atcagtatgt cagcccaaaa
      301 agaaatatca acaagcatat gacctttaca aactaagtta caattactcc ccgtatgatg
      361 actattcagt tatttatcgt atgggtcaat gtcagattgg ggctaaaaat atcgataacg
      421 caatgcaatg tttctatcac attattaaca attgtgagga tgatagtgtt aagagtaaag
      481 cgcaggcata tattgaactc ttaaacgata attcagaaga taatggctaa gcattacaat
      541 catttggtta aatttgctgg ttatagtgca gttaaccact ataaccagca cctcttatgt
      601 tttcttaaat cagcatctta aaacagttaa gagcatacta taaatcatta tttttcaatg
      661 cgttagagaa caatctactt tatcaaaatt gaataaggct atttcagatt acatcactga
      721 attcctaaga taaattagtg aacacaacca tagatatgtc gaatgtaatt tcctgtagcg
      781 ttaatgacac ataaatatga atagccataa tttctattgc ttaagcagga tgcaagaaac
      841 caattttttc atagaggtta actaatggac ttagaaaata aattctcata tcattttctt
      901 gagggattaa cgctcacgga agatggaatt cttactcaag gaaatgagca agtttatatt
      961 ccacagaaag agttaggtgt attaatagta ttacttgaat ccgctggtca tgttgtactg
     1021 aaagatatga tcatcgaatc agtatggaaa aatattattg ttagtgacga gtccctgaca
     1081 agatgtatct attctttgcg ctgcattttt gaaaaaattg gctatgatcg ttgcatagaa
     1141 acaatctacc ggaaaggtta tcgtttcagc gggcaggttt tcaaaactaa aattaatgaa
     1201 gataatactt cagactattc catagctata ttccctttca ctacttcgtt gaatacactg
     1261 gatccattaa tacttaatca ggaattagtg caaatcattt caaataaaaa aatcgatggt
     1321 ctctatacct atccgatggc tgcgacaaat ttttgtaatg atcacatatc tcaaaattca
     1381 ttcttgagca gattcaagcc agattatttc gttacaggaa gaataaacca gaataatgca
     1441 gtgaacactt tatacattga gttgatcgac gctaaaaacc ttttcctcat cgccagtaat
     1501 catctccctg ttgatgaact acataataca tcacaattta ttatagataa tatccttcaa
     1561 acggtacata aaccagaacg atctgtaaga ttagctaagc aggaccaagg atataagaat
     1621 cattatttat cagatgaaat gttagccgga aagaaagaac tttacgactt cacccctgaa
     1681 agcatttaca gggccatgac tatatttgat atattacaaa ataaaagtga tatacagacg
     1741 ctaaaaacag aatgttattg ccttctagcg gaatgccata tgtctttggc acttcatgga
     1801 aaaagtgaac ttgaacttgc tgctcaaaaa gcattagagc ttttagatta tgtatcagac
     1861 ataaccactg tcgatggaaa aattttagct attatgggac tgataactgg tctgtctgga
     1921 caagcaaaag tatctcatat cttatttgaa caggctaaga tacacccaac tgatatagcc
     1981 tctctctact actatagggc actagtccac tttcataatg aaaaaattga agaggcaagg
     2041 atttgtatag acaaatcact acaactcgaa cccagaagac gaaaagcagt tgtgataaaa
     2101 gaatgtgtag atatgtatgt gcctaacccg ctcaaaaaca acattaaact ctactataaa
     2161 gaaactgaga gtgaaagcca tcgagttata attgacaaca ttttgaaatt aaagcagctg
     2221 acgagaattt gtatgcgata aatttttatt gacgcaattt tattatttag atgtatgcaa
     2281 cttgttattt aaacaacaga gata
//
LOCUS       DAZFKM010000316         2242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFKM010000316 DAZFKM010000000
VERSION     DAZFKM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..486
                     /locus_tag="ACHKAY_005404"
     CDS             76..486
                     /locus_tag="ACHKAY_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703852.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPDFQILDNADHYGDF"
     gene            488..823
                     /locus_tag="ACHKAY_005405"
     CDS             488..823
                     /locus_tag="ACHKAY_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703853.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPASLNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGTITEE"
     gene            1173..1385
                     /locus_tag="ACHKAY_005406"
     CDS             1173..1385
                     /locus_tag="ACHKAY_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703854.1"
                     /translation="MLFLNDKEQIIKYKDEMLKINPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
     gene            1382..1744
                     /locus_tag="ACHKAY_005407"
     CDS             1382..1744
                     /locus_tag="ACHKAY_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703855.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYVSGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
     gene            1763..>2242
                     /locus_tag="ACHKAY_005408"
     CDS             1763..>2242
                     /locus_tag="ACHKAY_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5703856.1"
                     /translation="MILGNDYVDLIPLFQTHSTRNFLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYIAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     "
BASE COUNT          721 a          419 c          425 g          677 t
ORIGIN      
        1 attggttatt gttctttctc acgttagaaa catacttcgg ggtgttttcc tttaaaagaa
       61 aaggaaaagt aaataatggc aaaaagaaaa aacaacgttg ttaagaaaat cggggattca
      121 gcttctcttc taagtaaacc taaatctatt ctaagacgca aagactttaa agagctagtt
      181 acgctcacca agcaaaatag cgcccctggc gaatggaaaa cagagatcat agaacattct
      241 ccctctgtgc cttgcggaga tgaatttaac gccttacaag aaatcttatc ttcaacacct
      301 ggcgtattct ggaaacctag aaaaagaaaa gagtatatcg ttgatagctc tgatttacgc
      361 aaataccaga ttttaggatt tgaagactat aaccactacg ttggttatct cgccacaaat
      421 ggcctaaata acttagttcc agacttccag attctagata atgctgatca ctatggagac
      481 ttttaatatg aacaaaaaca cgtatgacac aatttactca ctaattaatt attatgagga
      541 tgattacctt ctgcctttaa accgcgctga acttgaagca tacaaagaaa acacaccagc
      601 gtcactaaat gaggcgttta aacactggga tttagctgtg aatgcctttg agcacctctc
      661 taagcgtgta gagatgctct gtaagcgcga aaacgcatac ctgacagccg atcagatatg
      721 ggagctatca aactggatag aagatatcga atctgatgtt cgctatgtgg gagatggact
      781 tgtcgaactc gctcaacgct taggcactat cacagaagaa taaaaaaagc aacaaaaaag
      841 ttgtaaaata tcttgacatt ccatctatta tatgttaata taattatatg gggtgattat
      901 ttttccttta ttggttcgat attaaaaatt cttcttgtcg ttaaagggga gtaaaatctc
      961 cgctccccgc ttctcctcct taagtaaata ctgtgtcatt attacctctt ttttcctttg
     1021 ttatgcatac atcttggagt atgcataaca tagggtaatt acataggtac aagttaataa
     1081 aaggcggctc taaatgctgc cttttatttt gcccgttatg caacttaaaa gttgtatttc
     1141 ttactttaaa caaggtaaca aggtttatat aaatgttgtt cttaaatgat aaagagcaaa
     1201 tcatcaagta taaagatgaa atgctgaaaa taaatccaca aattaccgaa atggtagcta
     1261 aatacgctgg gtgttctgta gaagaagtag aaagatgtgt tgaaaaatat ttttctcctt
     1321 cttctccgtc tacaccttcc cttaatgaat taatcaaatt aaaagctaag gagattacta
     1381 aatgattatt gatttagaca ctctgtttcc agttcgaaag actttcacag atattgtaag
     1441 ttattgtact gatccgtttg cctctgtaga aagcaaggtt tttgcttctc ttcctgctga
     1501 tgttgaatgc ggcgacttga tcaccagcac tggcgctaaa tatgtatcag gagatgatat
     1561 ctatgttgtt atgagtgaat ttgtaacggc tggagaaaac aaacctgtag atgttttacg
     1621 tagcaatgct gggcttgtat gcatcaaagc tgatgcgctg aatgctgtaa gtgaagcagc
     1681 aaaaacagca ttaattaaaa aaggcttcca gcttgaaggg ttccattctg ttttcacttc
     1741 ttaataaaag gaataataat aaatgattct aggcaatgat tacgttgatt tgatccctct
     1801 gttccagaca cacagcacgc gtaacttcct gctctctact cttgatttta cggattctgt
     1861 aggggttaca tctcataaag tagctgtttc ccagcttgtc gaaagcaacg aatctctctt
     1921 taacaaagag acaagccgtt ttagttctga acacaatgtt actaagcgag aacaaggtaa
     1981 agaatggctg atcgagatcc cttacttcct gcgtgaagat gtgatccgtc cttctgatgt
     2041 tcaaggcaaa cgtaaacctg gcactgattt ccaggaaaca ctcactgata tttatgcgga
     2101 atatatcgct aaacaccacg tagcgtatca gcgtacaaaa gagagcgtat tggctgcttc
     2161 tttgttctcg ggcaaaactt atacaccgaa aactgatgat gttcttatcg aatggggcaa
     2221 gctgtttaac gtatcagcta tg
//
LOCUS       DAZFKM010000317         2240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFKM010000317 DAZFKM010000000
VERSION     DAZFKM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(19..393)
                     /locus_tag="ACHKAY_005409"
     CDS             complement(19..393)
                     /locus_tag="ACHKAY_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703857.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHKAY_005410"
     CDS             complement(416..634)
                     /gene="ydfC"
                     /locus_tag="ACHKAY_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO5703858.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHKAY_005411"
     CDS             complement(664..792)
                     /gene="ydfB"
                     /locus_tag="ACHKAY_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO5703859.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(794..949)
                     /locus_tag="ACHKAY_005412"
     CDS             complement(794..949)
                     /locus_tag="ACHKAY_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO5703860.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1222..1938)
                     /locus_tag="ACHKAY_005413"
     CDS             complement(1222..1938)
                     /locus_tag="ACHKAY_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO5703861.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1988..2203
                     /locus_tag="ACHKAY_005414"
     CDS             1988..2203
                     /locus_tag="ACHKAY_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO5703862.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          565 a          484 c          490 g          701 t
ORIGIN      
        1 acctcacctg tgaataaatt aatgattaat tgatattttg ttgtttgatt caactttccc
       61 atcggatgtg tgatgcttta aatcacagga attaatactg cttgctgtaa aatgattttc
      121 aaggggagct attcgaatcc ctttcttttt cattaacaag ccaaatcctt tattaatgat
      181 gtccattaat tccagaaagt atttttcatg taaatcctgg ttatcagaga gctgcttctc
      241 ttcgtacagc ccgataaagg ctcggcgtac gttaccagat atattgtcga tggtttcttt
      301 ttctacggta ctcaggtcaa gagtcgccag ttgagagcga accacattcg ctgccatttc
      361 ctggaatggc attggtaaat ctttaaattc cattattagc ctcgttggtt agctattaac
      421 gcgggtatgt aatcattctg gcaatgctta atgccgctgc tttttccagc ctggtgatat
      481 cctgctccag agcggacaga ttttcagcct gcttagccct ggcttcattg gcccatttca
      541 ggtcctgcgc agccttaatt ttctggtgca tccactcata aagttcatca tcggtatagt
      601 ctggcgcgat gatgacgggt tctcgtttct gcatactgat tcctcgcggt gctgtttcgc
      661 ttatcagccg ttagattttg ccgaactgga aagcgcctgt ttaaactcac tgaagctgag
      721 ggcttcttcg ccttcggcaa ggccttcgaa gtattcttcg taagcctttt ccatgattgt
      781 gttgaaatcc atatcactca cctgagtttc tttccagcca gcgacgggca ccattttcgg
      841 ttttaaacgt tttgcttctg gtatacgtca tcgcggtgaa cgtaccgtcc tggttgggga
      901 acacgccaca taccagagat tcgctgttgc caagatcgat agtatccatg ctgacctcat
      961 ttccccttaa cgccggggta gcggaacaaa aacctgctgc atagttatta aagttgaacc
     1021 ctgccgtcat gttcttacgc ctcgggctgg ctacttaacc cctgaccact gccgggtaac
     1081 tcgaagtatt tccctgcgtt ctgtggggcg gggtgggttg gtatgttgtt aaggtaacaa
     1141 gagttacctt tcgagtcaat acaatgttgc aaaaggtaca tttgagggca taaaaaaccc
     1201 gcaatgaatg cgggttctga ctcagtctaa gtattgatgt atttgtgaaa ctttaccttt
     1261 aatggtgtaa ccaccattca gttcgatggg tttgtaaagc ggattcagtg acaacagata
     1321 gatgtttggt ccgtcaatcg caactttttt tagtgttacg tttggcgttc cttccaattg
     1381 gattaagatt atttttccca ccagttctct aatgttactt gagcatggtg tgatcagcac
     1441 ggtagatccg tcggggatgg ttgggaggcc gttagagttt gtcatcgcat ctccctcaac
     1501 atgcaataaa aaagagtttt cagcggtttt tgtcatgaca tcaacccagt tcttaatacc
     1561 aggaatcttg gttactggac aactcatatc ccaataacca gcctgttccc acgttaaaac
     1621 gggcaaccgg gcgatgttgt cactaatgta agggtactga ttcagacgca gatcatcggt
     1681 tttatcgtga ccgtcctttc cataaagaat ccattcagga gatttggaaa gcaattttga
     1741 cagtagatac aaattctcac cgtcaggttt tgaagagcca ttttcccatt ttgttacgga
     1801 tacacgagat atgccgattg ctttcgcaac ctgctgttgg gttaatccaa cgtcttttcg
     1861 acgattccga atacgttcgc tgatagtgtt tttcatgtaa ccaatgttac taccaagtga
     1921 tgttgctatg gttgacattg ttatgtaact attgttaccc ttctgctcga aataacagga
     1981 gagttttatg ttcaaagatg atgttctgcg ctatttcaaa aaaaagcgac tagtagctga
     2041 ggctcttgga atttcacatg tggctgttgt gcggtggaaa gcagttattc ccaaacttcg
     2101 cgcaatggaa ctggatgaaa ttactaacgg tgaattgaaa tacaacccag aactttacaa
     2161 gaagcaggat agcacctcga acgaaggaaa gaatgattca tgaaaatcaa gcatgaacac
     2221 atccgcatgg cgatgaatgt
//
LOCUS       DAZFKM010000318         2221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFKM010000318 DAZFKM010000000
VERSION     DAZFKM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..237
                     /locus_tag="ACHKAY_005415"
     CDS             <1..237
                     /locus_tag="ACHKAY_005415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703863.1"
                     /translation="IFALTFQNHTNCPLTDLGGKTCIFSHPVYLFLREFGLQDSRGGS
                     VDKCLLQRLSTSYKQWWFIALPHEEKWKQVIPVS"
     gene            884..1861
                     /locus_tag="ACHKAY_005416"
     CDS             884..1861
                     /locus_tag="ACHKAY_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO5703864.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            2045..>2221
                     /locus_tag="ACHKAY_005417"
     CDS             2045..>2221
                     /locus_tag="ACHKAY_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5703865.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREG"
BASE COUNT          592 a          547 c          527 g          555 t
ORIGIN      
        1 atattcgccc tgactttcca gaaccatacg aactgcccgt tgacggacct cgggggaaaa
       61 acgtgtattt ttagtcatcc tgtttacctc tttctcaggg agtttggtct ccaggattcc
      121 cggggcggtt cagtggataa atgcttatta caacgtttat cgacctcata caaacaatgg
      181 tggtttatcg ccttgccgca tgaagaaaaa tggaaacagg ttattccggt gtcctgattt
      241 tatgatccac tacacattcc tgacgaacgc cactgcataa atcacattgc acatcctcag
      301 agagtacgca gtataggagg ggtatatata cattatcggt atatcaggcg acttctgtct
      361 gtatgttgtc ggtgctcgcc gcagaccggt gaccgggcga agcccgcgaa ccggcgagga
      421 agcgcaatgt atctgtagtt acagtgagca gatacgccct gaagcttcct cataaaacag
      481 ttgtgtaaac tgttttcccg gaatgattta tccacatatc cggcggcccg atccagtaaa
      541 cagatccata aatgatcaag aaatgatcca ttaaagatcc ccatatcgct gcaaaccttg
      601 tcactcatgc tccgggacca cgatcacata agctgtggca tgtttctgat aaactgtagc
      661 aggatcatga taagctgtat tcagtaatgt atatactgta gtaagttaat gacataaatt
      721 atggtcagta cgccaggctc agctgttaaa tacagactgc aggtttttct tcagtcagtt
      781 gacggggttc tgacactcaa ttttctgttt attcttttac tgtccacagg caggaggctt
      841 tctggaaaac gaaaattcag acatcaaaaa actgttcggc gaggtggata agtcgtccgg
      901 tgagctggtg acactgacac caaacaataa caacaccgta caacctgtgg cgctgatgcg
      961 tctgggcgtt tttgtaccga cccttaaatc actgaagaac agtaaaaaaa atacactgtc
     1021 acgtactgat gccacggaag agctgacacg tctttccctg gcccgtgctg agggatttga
     1081 taaggttgag atcaccggcc cccgcctgga tatggataac gatttcaaga cctgggtggg
     1141 aatcattcat tcctttgccc gccataacgt aattggtgac aaagttgaac tgccttttgt
     1201 cgagtttgca aaactgtgtg gtataccttc aagccagtca tcccgcaggc tgcgtgagcg
     1261 catcagccct tccctgaaac gcattgccgg taccgtgatc tccttttccc gcaccgatga
     1321 gaagcacacc cgggaatata tcacccatct ggtacagtca gcctactacg atactgaacg
     1381 ggatattgtt cagttacagg ctgatccccg cctttttgaa ctgtaccagt ttgaccggaa
     1441 agtgctgctg cagctcaagg ccatcaatgc cctgaagcga cgggagtccg cccaggcact
     1501 ttacaccttt atagagagcc tgccccggga tccggcaccg atatcgctgg cgcggctgcg
     1561 tgcccgcctc aatctgaagt ctcctgtatt ttcccagaac cagacggtca gacgggcaat
     1621 ggagcagttg cgcgagattg gatatcttga ttacacggag atccagcggg ggcggacaaa
     1681 attcttctgt attcactacc ggcgtccccg gttaaaagcg ccgaatgatg agagtaagga
     1741 aaatccgttg ccaccttcac ctgcggaaaa agtcagtccg gagatggcgg agaaacttgc
     1801 cctgcttgaa aaactgggca tcacgctgga tgacctggaa aaactcttca aatcccgctg
     1861 aacataaact gtagtcagtg aagagtgctc ctttactgac tacagcttat attatcaggt
     1921 gcagtgagtg gtctgctcac tgcagtttat atgtaagcgt acagcctgaa ccgtctggtc
     1981 agaatctgac gaattagaca aagtggtgtc caccaaataa gtagtgggaa ccaaagtgtc
     2041 agatatgcag aaaaatgtga ctcccggcag gcgaaaaggc tgccctaatt atcctcccga
     2101 atttaaacag cagctcgttg ctgcctcctg tgaacccggg atatccatct caaaacttgc
     2161 tcttgaaaat ggcattaacg ccaatctgtt gttcaaatgg cgacaacaat ggcgcgaggg
     2221 a
//
LOCUS       DAZFKM010000319         2218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFKM010000319 DAZFKM010000000
VERSION     DAZFKM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.322"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..932
                     /locus_tag="ACHKAY_005418"
     CDS             15..932
                     /locus_tag="ACHKAY_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO5703866.1"
                     /translation="MNLLNSQLNMKINDFYLPLLSVIGTGRLYITPEGHACHAYFREV
                     SGNGIRFTLTCSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILEITVQSLQYLINENWQFVPHS
                     NTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECWLVINNPLATVKLCDNNL
                     FIADVQAADLLCTISNEAVMDRIYCVIGTIHVDIHMLRNAKKDDIIDSTGYHLFGGCR
                     LIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            963..1478
                     /locus_tag="ACHKAY_005419"
     CDS             963..1478
                     /locus_tag="ACHKAY_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO5703867.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
     gene            complement(1697..2056)
                     /locus_tag="ACHKAY_005420"
     CDS             complement(1697..2056)
                     /locus_tag="ACHKAY_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO5703868.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEINSNRDLLGMFFLSLKEDSVLLNIAFPADKVNITEFIN
                     LMENGYLLKNEVISLLS"
BASE COUNT          688 a          354 c          447 g          729 t
ORIGIN      
        1 agaaatcttt cctgatgaac ctattgaatt ctcaattgaa tatgaaaatt aatgattttt
       61 acttgccatt gttgtcagtc ataggaaccg gtcgcctcta tattacgcct gaagggcatg
      121 cttgccatgc atattttcgc gaagtcagcg gtaatggtat ccgcttcacg ttaacatgct
      181 caggctatga ggggcgtttt tggatatcag aagaacagtt cattcagtgg tgccaggagt
      241 tattcccgta tagcgaaagc cggctgatac cggaagatat gataaaactc atgatcttat
      301 gggtaatgca gactgcttta ccagaaggtg atgtgtctgt tgatgatgtt caatttacga
      361 tgctaaataa agatgtttat ccggtaatag aaaataataa tggagaaaac agacttaatg
      421 taattatact cgaaataacg gtacagagtt tacaatatct aattaatgaa aattggcaat
      481 ttgttccaca ttctaatacc attttctttg atggatatat tgctcctggg tggactgatt
      541 acccggttac agagctgact gttggtgata gtttaaggct ctatcatgtt gatgacagca
      601 aagaaagaga gtgttggctt gttattaata accctttagc aaccgtaaaa ctttgtgata
      661 ataatttatt tattgcggat gtccaggctg cagatttgtt atgtactatc agcaatgagg
      721 ctgtaatgga tcgcatttat tgtgttattg gaacaattca tgttgatata catatgttgc
      781 gcaatgccaa aaaagatgat attatcgatt caactggata tcatcttttt ggtgggtgcc
      841 ggttaatacg caataatacg actatagcat acggaagcat tgtaaaaatc aacgaagatt
      901 tttattttac tgttagttta gtatgtgatt aatcaggaat aagttatata gggagatgta
      961 ttatgtcgtc atcgttatca ggaataatac tcaatacttc gctgacaagt caccatatgt
     1021 catggggcaa gtttagcaga actcttcatg tattgaatga agatatcgta gatgaaattc
     1081 agcttaaagc ccgaacacaa caacgccacc atcgagtata ttcagaaatg gcggattact
     1141 ccgttgtctc ctttcggggc aaagaatatt ctgttcgttt tattaaagat tacaaaacag
     1201 ataattatgt gtacaaagta caaaagatga ctcaggaaag tgggtgtctc tcaagattat
     1261 ttagctttct ttctggcggt gtgactaagg ctttggaaag taaactaaat gagaggcata
     1321 tcactccact ttctccaacc tggtttcctc gaactccact ggagggggtc ttaacggaaa
     1381 gaggattatc ttctttgtta cgtcgcgtac agtctactga aaaccttgat tatagtaatg
     1441 ctatgatatc aaactctttc tctaactccg tagtgtgatt atcatttgag ctttcctcct
     1501 ggtttaactc cagaataatt gtgattctgg agtttttctg tttgttttat gtcgataata
     1561 cattgttttt aggaaaatat gggatgaata ttcagagtga gtttggctgg cagaagtcag
     1621 agattctgtg atgaggaggg gattgagctg aatggatgaa ttccttccag ctcataattt
     1681 atcaggataa aacatcttac gacaataaac taattacttc attctttaat aaataaccat
     1741 tttccattaa attgataaac tctgtaatgt taaccttatc tgcagggaat gcaatattca
     1801 aaagtacgct atcttctttt aatgatagaa aaaacatacc gagtagatct cgattgctat
     1861 ttatttctag tattttatca atttttgaag attcattatt gaataaataa caataaaaaa
     1921 acaggacctc agattgaggt gcacactcca gtatccattc atgaccatcg tgatcatgga
     1981 aagatagggc gttagtttgt tcttcaagag ttattccgaa cgtatgtgca agatctgtgc
     2041 gaaattgttc attcatattg tagtcctgtc attctttcaa tgagggtatg cttaaacgta
     2101 attaactcgt gagctctggc acaaataata agacgaaaat ataagatgca agtaatagtg
     2161 ttgcatattt atgatagcat gtgataaatg tagtgcaact acttgtagat agtgccgg
//
LOCUS       DAZFKM010000320         2206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFKM010000320 DAZFKM010000000
VERSION     DAZFKM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.323"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            353..1105
                     /locus_tag="ACHKAY_005421"
     CDS             353..1105
                     /locus_tag="ACHKAY_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO5703869.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            1102..1656
                     /locus_tag="ACHKAY_005422"
     CDS             1102..1656
                     /locus_tag="ACHKAY_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO5703870.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1658..1930
                     /locus_tag="ACHKAY_005423"
     CDS             1658..1930
                     /locus_tag="ACHKAY_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5703871.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          650 a          517 c          532 g          507 t
ORIGIN      
        1 tggtgaatat tggtgaggag aaaagatcca gtaataaact gaaataaata tcgcgactta
       61 tatgaaaaag aaatatatgt ttaattttat ctctgtgaac agtcatgaac agtagatgat
      121 cactttaatt aaaacactac accacttaac cacatgtatt tactatattt ttattgaagt
      181 gaacagtagt gaatagtatg tattaaagga agcgtgtaga tcagacatga cacctttctc
      241 tggctagcca gaacaaggtc attgttcagt cactgacaca atcctcctcg gtagtgcgct
      301 tgtatggacc ccggcacaat tgacacaaca acaaaacact accggagcag tcatgactac
      361 tgttaaccag atccctgatg caaacattac accctccctc ccaccgaaaa ttcgcgaagc
      421 ggtggataaa gttaaggcag caaaagctgt ctggcaggaa gaacgacgaa aacaaactga
      481 agctgctgca atgattgaaa ctatccgtaa acgtcaggaa gatacaaaaa cggaaacgca
      541 ggcgcttaat gatgaatggc gaaacctgtt tcgtgagaat cagggaaata tgacgccacg
      601 catgaaaaaa ctgcgggcag aaatcgccct cggacgcgaa acactggatg agttcgaaga
      661 tttgctggca gctcaggcgg cagaaaatga attcctgccc tggaaaactg cggatgctgc
      721 aaaccactac atcagcgaac ataatcgcct gattgaaact catgcagtgt ggctctggaa
      781 tgagtttatg aaggaacacg gtcagaaact tattcagatc cttggactgc tgaaaatgac
      841 tctgggccga agcgcttctg ctgttatcgg tgtagttcat accgtaaacg accccgaaag
      901 tgtgctgaag caatttatca gcgagcaact caccactccg gcactgtcct gtaacgtatc
      961 ttcaaccgat gatattaccc tgccggggat cagcatttat gcggacgata aagccatcca
     1021 ggatgccaga caatccccca gccctgcagc gcgttcacgg atgcttaaac agcgtgacat
     1081 ggttaaaggg ggcgagaaga aatgaatgcc ggaaccatta ctcaggaagc actcaacgat
     1141 taccgctcgg cgataaaaag ctggctgaca ctacgtaata cgcaaagtac ctaccagctt
     1201 cgtctggcgg ctctgctgga tactgaagaa aaaccggcag catatgccag ccagcttgag
     1261 gatcttcgtg agcgtcttgc agtcctcgaa tggcagatta actgtgctgc acgggatggt
     1321 ctttatgctc atcagattgt gctggagagt tgtgttacaa gcgcaacgga aaacttcatg
     1381 agcgagcatg gtgatgcact cactgacgct ctggctcctt ttctttgcgc tccatacggg
     1441 cttgaggcgg caatgaaaat attacgaacc gctgtagccc gacaaacgga aatgcgtact
     1501 ccggtaattt cagcagcata taagagcatt atcgacgaaa ccggattaac ggtggatgca
     1561 tcaatgcgcg ctgatgcttc agccagtttc accccggcac aacataaagt tttcctggcc
     1621 cgccttaacc gacttaatga aaaaggagtg tgctgatatg gccctgaagt gtcctgaatg
     1681 tggcgcggtc gctcacgcca gaaccagcgc ctatgaagct ccgtcggtta aacgctcatg
     1741 gtatcagtgc cagaatctgg aatgttcctg cacatttacc gcactggaaa gcgtggatac
     1801 gataattatg aagcctcggt gcaatgaaca ggaatcagac aaagcaaaaa taccggaaaa
     1861 acaacagcaa actctcaatc gctatggctc cgcgtcaaag ctgtcaaacc gtcagcaaat
     1921 tcctgtctga ttagcaaaat acgaccacga agtcccggtc cagtaccggg attttttacg
     1981 cctttttcct ggctggcctg agagcgtatg agtgcatatc tatggcgcat gaaaacgcat
     2041 gagtctcatg cgccattttt gacgcgaaag cccttgtgtg gtggcttctg agacgattta
     2101 cggggtgcat gaaaaccagt ctgttaagcg aagcgggcag gcgggcgggg cattgcgcgc
     2161 aggaatgtgt ataccgtttt agtaatgatc gctgacatct cacttt
//
LOCUS       DAZFKM010000321         2178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFKM010000321 DAZFKM010000000
VERSION     DAZFKM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..327)
                     /locus_tag="ACHKAY_005424"
     CDS             complement(<1..327)
                     /locus_tag="ACHKAY_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO5703872.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELM"
     gene            complement(479..1078)
                     /locus_tag="ACHKAY_005425"
     CDS             complement(479..1078)
                     /locus_tag="ACHKAY_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5703873.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDDPKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYDGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1146..>2178)
                     /locus_tag="ACHKAY_005426"
     CDS             complement(1146..>2178)
                     /locus_tag="ACHKAY_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5703874.1"
                     /translation="FFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNVSNEITQ
                     TVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPAG
                     MQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFS
                     LTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFP
                     RDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKN
                     GAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMK
                     KATAGITIS"
BASE COUNT          513 a          623 c          496 g          546 t
ORIGIN      
        1 catcagttca aaacgacgca gtgcctccgg ccggacgtca tcctccgaca tggcaccgag
       61 aaaatcattc agcgtccccg gttgagaatc ttcatacacg gtgatggtcc cggcatgtga
      121 cggaggaaat ccctccacca acagaatgac gctgtactga ccgtactcaa cgtccatgct
      181 gtaacgtccg gcttcatccg gattttcaga ggccaccgtg ttcaccacca ccgtgctgct
      241 ggttcgtctg gccttcagca caatggtgca gttctgtact ggttttcctg tgccatcttt
      301 aagcacgcca gaaattttta ctgtcatact tttccaccaa taaaaaaagc ccgcagcagt
      361 gacgccacgg gcttcaggac agtgtaactt tacgtttcct caaacgcagt tcaccccata
      421 aggtggatga acctgcgtat cataacaata tttacagaag ataaatcggc gtctgttgtc
      481 agaaacggta tccgatacca acaataaatg catccgatcg ccagtcgcca ctaccggaac
      541 cgtcataagc aaggtcaatg gtcacggatt cggtcgggtt aaactgcacg ccagcccccc
      601 acgccagaga cgtgttgctg tggcgaccgt catcacttcc ggtcagcaca tcgtgcgttt
      661 tccccttgtt gtcagttacg cggagataat ccccggagaa agtcgacaca cggctgtaag
      721 ccacacccgc catcgcatac gcgctgaacc attcattcac gcgtacagac ggccccgcca
      781 tcacgctgaa ccagcggtta cgcacggaat cttcatgcca gcgggtatcg ctgtagcgcg
      841 tttttgggtc atcctcagca ttggcataac tgaaggacgt aatcagcccc agcgcgtcca
      901 taaactcata acggtatttc acgttaatcc cgttcagatc atcactaccg ggaacgttcg
      961 tcgaggcatg gagatacccc gcgctcagcg tggactgatg ttctgctgca ctcgctggcg
     1021 tagcagcggc gacctgccag actactgcgg acaaaataac agcacataat ttacgcataa
     1081 ttacctctcg cttttctgca ataaaaaagg cgccatttct ggcgcccgta tctgggttat
     1141 aaaattcagc taatcgtgat gcctgcagtg gctttcttca tcaccacaac cagcaaatcg
     1201 ctgatacttg ctgtgggata ccagttattt accagccatg ctgacaccga aaactccagt
     1261 gtcatgtgac cgtgaccggc aggcatatca ataacaccac tgtaaatcag cgtattatcc
     1321 agcgcggtac ggttataaat ttcagcaccg tttttccgca ctatcagacg gcatgaggag
     1381 taaatatcag tatgctcttt ctcatgttta gcgccgctga atgccaccgc cggaataaca
     1441 atctgccggt caaacggctg atcgtcataa accctgacgg taatggttcc tgatggccac
     1501 cgctccggtg cacgggagtc ccgggggaaa gctttgccca ctgttttaac gagatcgcct
     1561 tcaatctggt tcgcggacaa ttttcccaga acccgacagt tctcgttaat cgtgacgttg
     1621 ttgagcgtcc cggagttcgc attcacgtta ccgctgatat cggcattttt cgccgtcagc
     1681 cgcccgtccg gtgtcaggga aaatgccgga ggattaccgc cgctggtaat ggtgggagcc
     1741 gtcagatatt tcaggaacac ttcattcata aatatctgat cgccctgacc aacaaacatc
     1801 ggctttgtgt tgccattcgc aggattaatc atcgcaatcc tgtctgccgc cagcagcacc
     1861 tgactctgca ttcctgctgg cgtattctca ataccggcac cgatacccgc aatataaagg
     1921 cgtccgtcct gcatctgctg cagtttcacg gcccacatgc tgttcaggtt attatttgta
     1981 tcaacctgaa ctttctgtat ctgctggatt gccgcactct gattttccag ttttttattg
     2041 acggtctgcg tgatttcatt gctgacattc gtaatggacg tcctgatttc agccaggtcc
     2101 ggcgcaagct gaccgttatc aatctgcgtc cacagctcct gggccagatg tgttttcccg
     2161 atttctcctt tgaaaaaa
//
LOCUS       DAZFKM010000322         2175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFKM010000322 DAZFKM010000000
VERSION     DAZFKM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1743
                     /locus_tag="ACHKAY_005427"
     CDS             <1..1743
                     /locus_tag="ACHKAY_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO5703875.1"
                     /translation="NIIMLLMSSLILSGCGPESENKESQQQQPSTPTDQQVLAAQQAA
                     IKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQI
                     ANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEE
                     IVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKH
                     ITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPL
                     GPTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFI
                     RLIELADGVTDISQ"
     gene            complement(1814..>2175)
                     /locus_tag="ACHKAY_005428"
     CDS             complement(1814..>2175)
                     /locus_tag="ACHKAY_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFO5703876.1"
                     /translation="SNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQR
                     ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQL
                     EIKHSQPKNRASGKRVS"
BASE COUNT          628 a          468 c          521 g          558 t
ORIGIN      
        1 aatataatca tgttgcttat gagtagtttg attttgtcag gatgcgggcc ggaatctgag
       61 aataaggaaa gtcagcaaca acagcccagt acccccacag accagcaagt gcttgccgcg
      121 caacaagctg caataaaaga ggccgagcaa agatccgtcg ccgataaagc cactgcagac
      181 gcgaaagcaa aagccttagc ccagcaagaa gcgcaacaat attcagacaa acaggctttg
      241 caggggcgct tacaggcggc tccaaaatat caacatgcag ccagagaaaa agcagcgtcc
      301 caaatcgcaa atccaggaac cgctcgctac aagcagttcg atgataatcc ggttaagcag
      361 gtagcgcaaa acccgttggc gacgtttagc cttgacgtcg acaccggcag ttatgcgaat
      421 gtacgccgtt ttctcaatca tgggctgtta cctccgccag acgctgtgcg ggtggaggag
      481 atagtcaatt atttcccgta tgactgggat atcaaagaca aacaatctat tccggccact
      541 aagccaatac ctttcgctat gcgctacgaa ctggcacctg cgccgtggaa tgaacagcta
      601 acattgctga aaattgatat cctggcgaaa gatcataaaa gtgaagagtt accagcttct
      661 aatctggtct ttcttatcga cacttctggt tcaatgattt ctgatgaacg tttgccactt
      721 atccagtctt cgttgaaatt attggtcaaa gaacttcgtg agcaggataa cattgccatc
      781 gtgacctacg ctggcgactc ccgtattgcg ttgccttcta tctccggtag tcacaaggcg
      841 gaaattaatg ccgcaattga ttcgctggat gccgaaggca gtaccaatgg cggtgccggg
      901 ctggaactgg cttatcagca ggcggcgaag gggtttatta agggcggcat caatcgcatt
      961 ttattagcca ctgacggtga ctttaacgtt ggcattgacg atccaaaatc gattgaatca
     1021 atggtcaaaa aacagcggga gtctggtgtt actctgtcga cgtttggcgt ggggaatagc
     1081 aattacaacg aggcaatgat ggtgcgaatt gccgatgttg gtaacggcaa ctacagctac
     1141 attgataccc tctctgaagc gcagaaagta ttgaatagtg aaatgcggca gacgttgatt
     1201 accgtagcaa aagatgtcaa agcgcaaatt gagtttaacc ccgcgtgggt aacggaatac
     1261 cgtcagattg gttatgaaaa gcgccaactt cgggcggaag attttaataa cgacaacgtt
     1321 gatgcggggg atattggcgc aggcaaacat ataacgttgt tattcgaatt aacgctgaac
     1381 gggcaaaaag catcaattga taagttacgc tatgccccgg ataacaaatc agtgaaatcg
     1441 gacaaaacga aagaactggc ctggttaaag attcgctgga aatacccgca gggaaaagaa
     1501 agtcagttag ttgaattccc gctggggcca acaataaacg ccccctctga agatatgcgt
     1561 ttttgcgcag cagtagctgc atatgggcaa aagttacgcg gttctgaata cctgaacaat
     1621 acctcctggc agcagatcaa acagtgggct cagcaggcaa aaggggaaga tccacagggt
     1681 tacagggcgg aatttattcg cctgattgaa ctggcggatg gtgtgactga catcagtcag
     1741 tgatgactgt ttagcaaact atgttcgacc agtcagcata tttgctgact ggtcgaattg
     1801 attaacaatg atgttaactc actcttttgc cggatgctct atttttgggt tgtgagtgtt
     1861 ttatttctaa ttgtgtataa gaagcattga ttcgtgcatc gccatattta tctaaataac
     1921 aatttctgtt taccagtcga tgttgaaaat caatttcgta ctgctcttct gctgaatatt
     1981 ttttaaatct gtcagcatag ttttttaata acgtataggg ggcgctgtca gaattgcttt
     2041 ccctttgttc aatcacttcc tgggctgcca tgcacacaaa cgcaccgcaa ctttggctta
     2101 agtattgctg aagatcggca tgtaaaaata ttatctctgg ttcatttaaa tgaactttta
     2161 tattattatt cgaca
//
LOCUS       DAZFKM010000323         2171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFKM010000323 DAZFKM010000000
VERSION     DAZFKM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..184)
                     /gene="rrrD"
                     /locus_tag="ACHKAY_005429"
     CDS             complement(<1..184)
                     /gene="rrrD"
                     /locus_tag="ACHKAY_005429"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5703877.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
     gene            complement(184..399)
                     /gene="essD"
                     /locus_tag="ACHKAY_005430"
     CDS             complement(184..399)
                     /gene="essD"
                     /locus_tag="ACHKAY_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO5703878.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            988..2070
                     /locus_tag="ACHKAY_005431"
     CDS             988..2070
                     /locus_tag="ACHKAY_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO5703879.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          671 a          468 c          437 g          595 t
ORIGIN      
        1 gcatgatgtc ttttcctgta tgcccgtgac atacagtcca tacaccaaca atatctttgt
       61 atggtatgta gctgacacct tccagaccat cgttaccact tgggccagtg attaacactg
      121 atgctatagc aattgctccg ccaccaatag cagcagcaac ggcttttcgt aatgatggag
      181 gcattattca cctctcgcag ccttgcgctt atcttcttta atcttgaaat aaaggtttgt
      241 caggtacgtc agcaggccaa ataccaggct acccagcaca cctattgctg cccactgtga
      301 gggcgtgact ttatcgagca gctgtaaaaa ccagtaaccg gcactacctg ctgaggtgcc
      361 ataggcgaca cccgttgtta acttatccat ggatttcata accccacctc gcagacaaag
      421 cgggtgtaaa ttgagggaat acaacgtatc gcaaaaaagc agaaacgtaa cagactcgga
      481 gtcagtgaat aactcaggta ttgggttatc agctaatatc gagactcaaa aaatggaaaa
      541 acccgctcga cggcgggttt aagctgtgtg acgaagtaac cactcttaac agcataacca
      601 attttttacg tacgtaaacc actaaatgat atttgcgaga atgctaccga gtattgaaaa
      661 caccactaca aatacataaa caaatctcaa caaataacca acaaataatt tccagcgtta
      721 tttttagccg atttaaattg aaccttcaaa ttatagggca cttataaata acagccatta
      781 atataaattg gctaatagat ttatttttat tcagccaaga gccatgaata ggattcgata
      841 gaaaaagttc agataaaaat agagatctac ttcacaaatc aaatgagaaa ccaaaactta
      901 catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta
      961 ataatagttc aaaaaaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta
     1021 gctgcatcag tactgatggc gatgtctgct caggcagctg aaatttataa taaagacagt
     1081 aacaagctgg atctgtacgg gaaagttaat gctaagcact acttctcctc taatgatgca
     1141 gatgatggtg atactactta tgcccgtctt ggcttcaaag gtgaaaccca aatcaacgat
     1201 caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgcgc tgaatctcaa
     1261 ggttcctcca aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc
     1321 tccatcgatt atggccgtaa ctacggtgta gcatacgata ttggtgcatg gaccgacgtt
     1381 ctgccagaat tcggtggcga tacctggacc caaacagatg tattcatgac tggtcgcacc
     1441 acaggtgttg caacttatcg taacaatgac ttctttggtc tggttgatgg tctgaacttt
     1501 gctgctcagt atcagggtaa aaatgaccgt actgacgtaa ctcaagctaa tggtgatggt
     1561 ttcggtttct ccactactta tgagtatgaa ggtttcggcg taggtgcaac ctatgctaaa
     1621 tctgatcgca ctaataatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa
     1681 aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctact
     1741 acctattctg aaacccaaaa catgactgtt tttggtaaaa accatattgc caacaaagca
     1801 caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct
     1861 tatctgcaat ctaaagggaa agacttgggt gcttggggtg atcaggacct ggttgaatat
     1921 attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgactacaaa
     1981 atcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc
     2041 gttgctgtag gtatggttta ccagttctaa tttgattact aaaacatatg ttgtgggagg
     2101 ctttgcctcc ccaacatata agtggctccc tcaagccact tcctttagaa gcacaacctt
     2161 gcttctaact a
//
LOCUS       DAZFKM010000324         2155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFKM010000324 DAZFKM010000000
VERSION     DAZFKM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1233
                     /locus_tag="ACHKAY_005432"
     CDS             <1..1233
                     /locus_tag="ACHKAY_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5703880.1"
                     /translation="PKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLADMAG
                     QCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENGVNV
                     PTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAILRHA
                     GRVALNAGASSTVSEVRPSSPSGAEATGVTALLSYLASESEGSLKVQGWSASGGRAEV
                     VSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYV
                     MAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGA
                     FDNEWHTLAFRFAGNNSLQVTPVIDGQVGTPFTLTQSPVSAFAADKLHVTDITRGATY
                     PVLIDSIAVEVNSTDTAA"
     gene            1369..1548
                     /locus_tag="ACHKAY_005433"
     CDS             1369..1548
                     /locus_tag="ACHKAY_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO5703881.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            1589..1861
                     /locus_tag="ACHKAY_005434"
     CDS             1589..1861
                     /locus_tag="ACHKAY_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO5703882.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            1938..2153
                     /locus_tag="ACHKAY_005435"
     CDS             1938..2153
                     /locus_tag="ACHKAY_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5703883.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
BASE COUNT          545 a          498 c          654 g          458 t
ORIGIN      
        1 ccgaaaaatg tgctgtttgc cgtggtgtgg atgcaggggg aatttgattt tggcggtacg
       61 ccggtaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctggcg
      121 gatatggcag gtcagtgcgt cggtggctct gctggcggtg ttccctggat atgtggagat
      181 acgacgtatt tctggaagca gaagaacgaa tcctcgtacc agacggtgta cggcagctac
      241 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgacggatga gaacggggtg
      301 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
      361 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagt
      421 tcatgggctc gccgcgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg
      481 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccgtcatcg
      541 ccttccggtg cagaagccac aggcgtcaca gcactgctct cttaccttgc cagcgagtca
      601 gagggaagcc tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc
      661 gatgcggagg gaaccggagg taaggcagtg aagctgacca aggaagccgg taaaagcagc
      721 tgggtgctgg agtacgccgc gggcaacggt gcggctctgt tacagaaagg ggggcagatt
      781 cgctgccgct ttaaggtttc gggagcgctg gctgcgaacc agtatgttat ggcgttttac
      841 tggccggtat cttcactgcc acagggcgtt gccctgaccg gagacggggg gaataacctg
      901 ctggcagcgt tctacatcca gacagatgca aaagacctga atgtgatgta ccacaatgcg
      961 aaagtggcga caaacaacct gaaactggga acctttggcg catttgataa cgaatggcat
     1021 acgctggctt tccgctttgc cgggaataac agcctgcagg tgacgccggt tattgatggt
     1081 caggttggca caccgttcac gctgacgcag tcaccggtca gtgcctttgc ggcggataaa
     1141 ctgcatgtga cagacattac cagaggtgcg acttacccgg tactgataga cagcattgcg
     1201 gtggaagtga acagcacaga cactgcggca tgataaaaaa accgccagcg acaggaatgg
     1261 acgctggcgg tggtgatacc tatggagaaa aaataaagga acgatacttt cgtactctgg
     1321 ttttttaatg aaaacagttc ttattgtcaa caataacgga aagaaattat gacatttctg
     1381 aaccagttaa tgctgtactt ctgtacggtg gtctgtgtgc tgtatctcct ttcgggtgga
     1441 taccgggcca tgcgtgacgt ctggcgcaga cagattgaca aaagggccgc tgagaaaatc
     1501 agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcatttagcg gcaactttct
     1561 taatcacatc tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga
     1621 agcagtgaag gaagtcctgc gctctgaaga agtccggagc gcactgaaac aaaaacttcg
     1681 ccataacctg gaagcgcgtc ttgatgcaga ggttgatgcc attctggatg agctgcttgg
     1741 tgtacaggca gagccaccga ctgaagcggg agataccacc gcagagagcg gtgaagttca
     1801 gcctgaatca ccggtcgccg atgcgactga acctcaaccc gaatcggtca tgatgctgta
     1861 acggggagtc agggccatca gtagttaact ggtggctttt ttattgttgt cagcttccgg
     1921 ataacgggag acggggtatg taccagatgg aaaaaatcac aacgggtgtg tcatacacca
     1981 cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc tctccgtccc
     2041 agtgggtggc aatcggtgtg ctggggagtc tgctgtttgg cctgctgacg tatcttacta
     2101 acctgtattt caaaatcaga gaggaccgtc gtaaggcggc gcggggaaag taaag
//
LOCUS       DAZFKM010000325         2128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFKM010000325 DAZFKM010000000
VERSION     DAZFKM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>2128
                     /locus_tag="ACHKAY_005436"
     CDS             <1..>2128
                     /locus_tag="ACHKAY_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO5703884.1"
                     /translation="INGDILQHYINTHYLSESQTQKIKDIVDFLGIQDNTKRVKLESD
                     IKHISEVQQPLHSIISRQKEHVKNLLSGLLNEFSNKLRQQGLSLKTNVLSVNNFKASK
                     INSDTVEVTVTDLQGRLYSVDIDTRVIGLTFKEGINSLSEALEHMNIDAIMSVIGLVQ
                     YTRMIKTNDNISAIDHAGAVSDIKNIVDKFLGGILTFTNNRVYNPGGISGASLEGFIS
                     SGLEVCASRIGGTAGRYLSNVAKVIKLPLLDIGINIWSLYDSSLNHARATTQIEYIST
                     AIDVSFSSINTALSIGAIAYPPLAIAIVPITIFSHEVKNYAVYVNQINERHKLWLEAE
                     KYLDNGSAKVLSINKDTGIIDLSNNQVLGNVHLDMRENPPILHGEKSYNSGKNIGSHP
                     DMTDREIMESGAYNFACTKLSDAGEPDIFGWGDKDVCNSMELSESQLANGYSNRQWPS
                     QSPVIPEGIYNTVYLGYGETLRANTEVTLSSTGYFYEIARAYTDDELSEPLLTVCNQR
                     SSVIGGKEPLTIIIPALEHGMLGSNLHMIERFKNYNFSISGGKGGIKLMVGGIGDYNI
                     ECTPGVRNIISFEQLSRDFNLDLDLSDERKQNFYFHHPSGFYSGKVMSITQKGINSVV
                     GTKQGYDKIRGNNLDNTFSLGNGGGVIYSGGGSNTYFVPATLQHNLHIYISEKSNGNH
                     IILGETHSRLSIECHFSDDKREFIKM"
BASE COUNT          741 a          313 c          416 g          658 t
ORIGIN      
        1 aaataaatgg ggatatttta caacattata taaatacgca ttatttgtca gagtcgcaaa
       61 ctcagaagat aaaagatatt gtggattttt taggcattca ggacaacaca aaaagagtta
      121 agcttgaaag tgatattaaa catataagtg aagttcaaca gccccttcat tctattatct
      181 cacggcagaa agaacatgta aaaaacttac tatctggatt acttaatgag ttttcaaata
      241 agttaagaca gcaaggcctt tcacttaaaa caaatgttct ttcagttaat aacttcaaag
      301 catcaaagat aaatagtgat actgtggagg ttacagtaac tgatctgcaa ggaagacttt
      361 atagtgttga tatagataca agagttattg gcttaacttt taaagagggg ataaatagtt
      421 tatctgaagc actcgaacac atgaatatag atgcaattat gtctgttatt ggcttggttc
      481 aatatacacg aatgataaaa acaaatgaca atattagcgc aatagatcat gcaggagcag
      541 tatcagatat taaaaatatt gtagacaaat ttttaggcgg aattttaact tttactaata
      601 atcgcgtata taaccctggt ggtatatctg gtgcctcgct tgaaggattt atatcttctg
      661 gtttagaagt gtgcgcgtca agaataggtg gcaccgctgg gcgttatctt agcaatgtgg
      721 caaaagtaat aaaattacct ttgctagata taggcataaa tatttggtca ttatatgact
      781 cttcattaaa tcatgccaga gccaccacac aaattgaata tatatcaaca gcgatagatg
      841 tatctttttc atctataaat actgctttat caataggcgc aatcgcatat ccacctttgg
      901 caatagctat agttccgata actatctttt ctcatgaagt aaaaaactat gctgtttatg
      961 tgaatcaaat taatgagcga cataagttat ggcttgaagc tgaaaaatat ctggacaatg
     1021 gaagcgcaaa ggttctaagc attaacaaag atacaggcat aatcgattta tcaaataatc
     1081 aggtgttagg taatgttcat ttagatatga gagaaaaccc tccgatatta catggtgaaa
     1141 aatcatataa tagcgggaag aatattggaa gtcatccaga tatgactgac agagaaatta
     1201 tggagtctgg agcatacaat tttgcatgta caaaactctc agatgcaggt gaacccgata
     1261 ttttcggttg gggagataag gatgtatgta attcaatgga actgtctgaa tctcaattag
     1321 ctaatgggta ttctaaccgc caatggcctt cacaaagtcc agttattcct gaagggattt
     1381 ataatacagt atatcttggg tatggagaaa cattgcgagc taatacagaa gttacattga
     1441 gtagcactgg ttacttttac gagattgcaa gggcatatac tgatgatgaa ctatctgagc
     1501 cattattaac agtatgtaac caacgtagta gtgtaattgg tggaaaagaa ccattaacaa
     1561 taatcattcc agctcttgaa catggtatgc ttggctctaa tctacatatg attgaacgtt
     1621 ttaaaaacta caatttttca atatcaggag ggaagggcgg gataaaatta atggttggtg
     1681 gtattggtga ttacaacata gaatgtaccc ccggtgttag aaatattatt tcttttgaac
     1741 aattatcacg tgacttcaat ctggatttgg atttatcaga tgagaggaaa caaaattttt
     1801 actttcatca tccctctggt ttttattcag gaaaagttat gagtataacg caaaagggca
     1861 taaattcggt tgttggtact aaacagggat atgataagat tcgtggtaat aatttagata
     1921 ataccttttc ccttggaaat gggggggggg ttatatattc tggtggagga agcaatacat
     1981 attttgttcc tgctacacta cagcataatt tgcatatata tatatcagaa aaatcaaatg
     2041 gtaaccatat tattttaggt gagacacact ctcgcttatc tattgaatgc cactttagtg
     2101 atgataaaag agagtttata aagatgga
//
LOCUS       DAZFKM010000326         2102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFKM010000326 DAZFKM010000000
VERSION     DAZFKM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..356)
                     /locus_tag="ACHKAY_005437"
     CDS             complement(<1..356)
                     /locus_tag="ACHKAY_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5703885.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVP"
     gene            484..1344
                     /locus_tag="ACHKAY_005438"
     CDS             484..1344
                     /locus_tag="ACHKAY_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO5703886.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNMKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQIEDIISVINWAEKQACIDNQRIG
                     LWGTSLGGCHVFSAAAQDQRVKCIVSQLAFADGDALITGEMNELERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLNDDESKVFFEKVKAQHPEMDIKIPFLTVMETLQYKPAESAA
                     RVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGTFFERVAA
                     VQTQWFKKYI"
BASE COUNT          562 a          436 c          524 g          580 t
ORIGIN      
        1 ggaaccagac ggacttcatc ttttggcggg cgtccgcgtc gcgcgcgcac tttttcggac
       61 aggatccgca caaacggtct tacaccatcc agatcaaatg attccggtgt cagcatccgg
      121 gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg gtgacgctga
      181 tccggcatat agaaaagaag ggcactcact tccggggcca gcaggtaggc cggtgtggcc
      241 cctgtggaag tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca
      301 atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt
      361 tatttttcac gtagcacacg cactgtttat ggatactgat tgcgctgggc attctggtat
      421 tgtgactggt atgatgctgg cgctgattct gatgattaat aaacaacata acacaggata
      481 tttatgaaaa ttacggatca caaactttct gaaggtattg ctctgacatt tcgtgttcct
      541 gaaggaaata tgaaacatcc tttgattatc ttatgccatg gattttgcgg tatccggaat
      601 gttctgctcc cttgctttgc gaatgctttc acagaagcag ggttcgcaac gattactttt
      661 gattatcgtg gattcggtga gagtgaagga gaacggggcc gtctggttcc agcaatgcag
      721 atcgaagata ttatttcagt tatcaattgg gcagaaaagc aggcatgtat tgataatcag
      781 cgtatcgggt tatggggaac atcactgggt ggttgtcatg tgttcagtgc agctgcgcaa
      841 gatcaaagag tgaaatgtat tgtcagccag ctggcttttg ctgatggtga tgctctgatt
      901 accggagaga tgaatgaatt ggagagagct tcttttctgt ccacactgaa taaaatggca
      961 gagaaaaaga aaaacacagg gaaggagatg tttgtggggg ttacacgggt actaaatgat
     1021 gatgagtcga aggtattctt cgagaaagtc aaagctcagc atcccgaaat ggatataaaa
     1081 atacctttcc ttactgtcat ggaaacgttg caatataaac cggccgagag cgcagccaga
     1141 gtccagtgtc cagtattggt tgttattgct ggccaggata gtgttaatcc accggaacag
     1201 ggaagagcat tatatgatgc tgttgcttct ggtactaaag aattatatga ggaagctgac
     1261 gcatgccatt atgatattta tgagggcaca ttttttgagc gggtagcagc tgtgcaaaca
     1321 caatggttta aaaaatatat ttaatagtga gcctgtgttt accattctgt gtaaatactt
     1381 tttcttaaaa gtgaccgtca aggcggtcac taaactttat aataaagagg catcgttcat
     1441 ttccgggagg cagactggat tgccagcccc ccacttttta ccaagctgca ggtatggggg
     1501 acacggacac tggtgttcac agaaaaaaat aaccatctct gaaatgtctg aaccgccccg
     1561 gttttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt ttctgatgga
     1621 agcataataa gctttttctg cttctgccgg agggatatga cccagccttc aggaaaaccg
     1681 gggcggttca aaccactttt gcaccgatcg tgacattttc ccttaataaa gcatctgtaa
     1741 ctgaaacaat cagcatatta ataatatatt gacaggcatc attgctgtct gtgaaaaata
     1801 agtatctaca aacatgcaaa gccttttagc cagtgtcttc ttcaggtcag tcgctggccc
     1861 ttttttatca tgatgtaggt gtatttatct ccagcaccag actttctatc tcaacgccat
     1921 acgctgcatt ttttgtaaca tccgtcagca tcagcactat gctgacgcat gacgtggagc
     1981 ctgtgattga cacgctgaag tcagtcagaa ggttgttcag taatcaggtg accgcctcct
     2041 gcctgcgcct gtctgctgga gtcatagaag aattacctgt taacgacggc gatatcatga
     2101 ca
//
LOCUS       DAZFKM010000327         2085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFKM010000327 DAZFKM010000000
VERSION     DAZFKM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHKAY_005439"
     CDS             <1..480
                     /locus_tag="ACHKAY_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5703887.1"
                     /translation="LNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLRQSGRV
                     AHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELES
                     RGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYVGA"
     gene            480..806
                     /locus_tag="ACHKAY_005440"
     CDS             480..806
                     /locus_tag="ACHKAY_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO5703888.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVAAMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTTVKDDDSEAAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            803..1192
                     /locus_tag="ACHKAY_005441"
     CDS             803..1192
                     /locus_tag="ACHKAY_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5703889.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDRQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            1212..2009
                     /locus_tag="ACHKAY_005442"
     CDS             1212..2009
                     /locus_tag="ACHKAY_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO5703890.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          511 a          513 c          609 g          452 t
ORIGIN      
        1 ttaaatgacg ttcagaaatt cattgaagcc catccggggt gtacttccgg agacattgcg
       61 gatgcttttg caggttactc acggcagcgc gttctgcagt ctgcaagcaa gttacgtcag
      121 agtgggcgtg tggctcaccg ttgtgaagga gatacacgca gacatttccc gcgcctgact
      181 gagagagcgc aggagccgga accacaacca gttcgtgaaa ccagacctgt gcgcaatttc
      241 tatgtcggca ctaacgatcc ccgggtgatt ttgtgcctga cccgccaggc ggaagaactg
      301 gagtccaggg gcttataccg tcgtgctgca acggtgtgga tggcggcatt ccgtgaaagc
      361 cactcccagc cagaacgaaa caattttctg gcgcgtcgtg agcagtgttt acggaaaagc
      421 agcaagcgcg ctgtatcggg tgatgagtgg tatctgtcag ggaattacgt gggggcttaa
      481 tgacgacgtt aactcaatgc cagcagcagg tgctggatat gctgatttct tatcagaaag
      541 aacgtggttt cccgccaacc aatcaggagg tggcagccat gctgggatac cgttcggtga
      601 atgcagcggt ggagcatctt cgcgcactgg agaaaaaagg cgtcatcacg ataaagcgtg
      661 gcgtggcccg gggtatcacg cttcatacca cagtgaagga cgacgacagc gaggcagccg
      721 ggattatccg cgcactgctt gccggtgagg aaaacgccag actgcgtgca gcccactggt
      781 tacatgagag gggcctgaaa gtatgaagct gatcctgcct ttcccgccca gcgtgaacac
      841 gtactggcga caccccaaca aaggggcgtt tgctggtaag agtctgataa gcgcagcggg
      901 gcgcaaattc cagagcgcgg catgcgcagc aatagttgag cagttacgtc gtctgccgaa
      961 accaacgtcg gcacctgctt cagtggagat cgtgttgttt cctccggata accggatccg
     1021 cgatctggac aactataaca aggcgctgtt tgacgccctg acccacgcgg gtgtgtggga
     1081 agacgacaga caggtgaaaa gaatgctggt ggagtgggga ccggttatcc cgaaagggaa
     1141 ggtcgagatc actatcagta agtatgagaa accggcgggt gcagccgcct gattaagagg
     1201 agaaacgaag tatgaataat ctgatggtta ttgatggtat tgaagttcgt cgtgatgctt
     1261 atgggcgtta cagcctgaac gatctgcaca gggctgctgg ttctctggat aagcataagc
     1321 ctgcattctg gctccgcaat gagcaaactg agcgtttaat aagcgagttg cagatttgca
     1381 actcggtcaa tatagagcca gttaacgtta ttcgtggcgg aaataaccag gggacgtatg
     1441 tctgcaaaga actggtgtat gcctatgcaa tgtggatcag cccgtcattc catctgaagg
     1501 tgatccgtac tttcgacatg gtaaccagcg caccggaaaa attatccgga caggctgctg
     1561 acaagatgca ggctggtgtg atcctgctgg actttatgcg ccgggaatta aacctgtcta
     1621 actcatcagt gcttggagcc tgtcagaagc ttcaggaggc tgttggctta ccgaatctgg
     1681 caccgcgcta tgccattgat gctcctgctg acgcgcctga tggctcaagc cgccccacgc
     1741 tgtcactgag tgcactgctg aagcagtatg gtatccgcct gacagctaat caggcatatc
     1801 accagatggt gaagctgggg atcgtcgagc agcgcgaacg atacagccgt accgcgatta
     1861 acaacatcaa aaaattctgg tcgctgacag cgaaaggctg catgttcggc aagaatatca
     1921 ccagtcccgc aaatccgcgc gagacgcagc cgcatttctt cgaatcccga ttccctgagc
     1981 tgttaaagct gctcgatacc gttcattgag gtgaccgtga gagcactact gacccctgaa
     2041 attgccccgc gtatggggat cgtattgttc aggccaggtt cagag
//
LOCUS       DAZFKM010000328         2052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFKM010000328 DAZFKM010000000
VERSION     DAZFKM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /locus_tag="ACHKAY_005443"
     CDS             <1..183
                     /locus_tag="ACHKAY_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5703891.1"
                     /translation="PTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLT
                     PEMLRALIREMKGGTR"
     gene            180..527
                     /gene="tnpB"
                     /locus_tag="ACHKAY_005444"
     CDS             180..527
                     /gene="tnpB"
                     /locus_tag="ACHKAY_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5703892.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            547..2052
                     /locus_tag="ACHKAY_005445"
                     /pseudo
     CDS             547..2052
                     /locus_tag="ACHKAY_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          510 a          569 c          592 g          381 t
ORIGIN      
        1 ccgaccagac cagttacaaa tcccttctgg cgtaacaagc ctgatgagtg ccctgagagt
       61 gatcccggaa acgtcccacg gtgcgagctg catcttaaat caggtgtggt aaaactgttt
      121 gaccctctca ctccggaaat gttacgggcg ctaatccgcg aaatgaaagg aggtacccga
      181 tgataacgct gccaaccggt accagaatct ggatcatcgc tggcatcaca gatatgcgtt
      241 gtggcttcaa cggcctggct tcgaaggtgc agaacacgct gaaagatgac ccgttctccg
      301 ggcatatctt cgtcttccgg ggccgcagtg gcaaaatggt gaaaatactg tgggccgatc
      361 gtgacgggtt atgcctgttc gccaaacgcc tggaacgggg ccgcttcgtc tggccggtaa
      421 cccgggaagg gaaagtgcac ctgacgccag ctcagttatc catgctactg gaggggatcg
      481 cgtggcaaca tcccaaacgg acagaacggc ctggcatccg gatataaccc gtgataaaac
      541 aggggaatga acaacacact ccccgacgac atcgagcaac tgaaggccct gctgatcgca
      601 cagcaggctg ttatcgtctg tctggtgaaa taaccggcta tgcccgcgag atcagctcac
      661 tcagagcgct ggtcgctaaa ctgcagagaa tgttgttcgg tcgcagcagc gagaaaagcc
      721 gcgagaagat agaaaagaag atcgcacggg cagaaacgcg tataaccgag ctccagaaca
      781 ggcttggtga ggcgcagttg caactcacct caatggccgg agagacagcg ccgaaaacat
      841 cagactctcc cgtccgcaaa gcacttccgg caacacttcc ccgtgacagg caggttatct
      901 ccccggcaga aaccgaatgc cccgtctgca gcggcaaact gaaaccgctg ggagaaagca
      961 tctctgaaca actggatatc atcaacaccg cgttcagggt aatcgaaacg gttcgcccaa
     1021 aactggcctg cagccggtgc gactgtatag ttcaggctcc gcagccacca aaacccatcg
     1081 agcgcagtta cgccagtccg gctctgctgg cccgcataat catggctaag ttcgccgagc
     1141 atctgccgct gtaccgtcag tcggaaatct atgcccgcca gggcgtggag ctgcaccgca
     1201 atacgatggg gcgctgggtt gacatcatgg gagagcagct tcgcccgctg tatgatgaac
     1261 tgaagcacta tgtgctgatg gcgggtaaag tgcatgccga tgacacgccg gtaaatgtac
     1321 tggagccggg tcagggtaaa acccgtaccg gacggctgtg ggtctatgtt cgtgacgatc
     1381 gcaacgccgg ttcgaccatg ccggcagcgg tgtggttctc atactctccc gaccgcaaag
     1441 gcatccaccc acagcaacat ctggcggact acagaggtat cctgcaggcc gatgcatatg
     1501 cgggttacaa tgctctttac gaaagcggtc aggtaaccga agcggcttgt atggcacatg
     1561 cccgacgcaa gatccacgat gtacatgtcc gccatccaac gacagtaacg ggagaagcgc
     1621 tccgtcgtat cggggaactg tacgctatcg aggctgagat ccgcggcagt ccggcagaag
     1681 agcgactggc ggtcagaaaa gccagaacgg taccgctaat gcagtcgttg tatgagtggc
     1741 tccaggggca gatgagcacg ctgtcgcgcc actcggatac agcgaaagcg ttcacctatc
     1801 tgctgaagca atgggacgct ctgaacgaat actgccgcaa tggctgggtg gagatcgaca
     1861 ataacctgtg tgaaaacgcc ctccgggtag ttgcactggg gcggcgtaac tacatgttct
     1921 tcggctctga tggtggaggc gagagtgcgg cagtgatgta cagcctgatc ggtagctgca
     1981 aacttaacgg aatcgagccg gaaacgtggc tacgccacgt gatcagtgta atcaacacct
     2041 ggcctgccaa cc
//
LOCUS       DAZFKM010000329         2043 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFKM010000329 DAZFKM010000000
VERSION     DAZFKM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..1162)
                     /locus_tag="ACHKAY_005446"
     CDS             complement(20..1162)
                     /locus_tag="ACHKAY_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFO5703893.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKVASMKNSNEIYTDGQNKALDNKTLEFKK
                     QLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVSSLMSKVGDVANKVGSNIVKTVTK
                     LADVFVENVVSKVAATANGFTTSARSIGTTVLNNDSAQYSILAQLSAYAVQNLTRQSE
                     NLGESAKVELDKAAAELRNQASYLQNASQLISDSARVNSRIVSGRV"
     gene            complement(1175..1753)
                     /gene="espA"
                     /locus_tag="ACHKAY_005447"
     CDS             complement(1175..1753)
                     /gene="espA"
                     /locus_tag="ACHKAY_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO5703894.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(1811..>2043)
                     /locus_tag="ACHKAY_005448"
     CDS             complement(1811..>2043)
                     /locus_tag="ACHKAY_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO5703895.1"
                     /translation="ILNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFF
                     ASLPDACFIDREQRDNALLMIGKVIDYKEDVI"
BASE COUNT          564 a          481 c          390 g          608 t
ORIGIN      
        1 attaaattct ctttaataat taaactcgac cactaacaat acggctattt actcgtgctg
       61 agtcggatat taactgagaa gcattttgta aatagcttgc ctggtttcgc aactcggctg
      121 cagctttatc cagctcaacc ttcgcactct cacccaggtt ttcactctgt cgagttaagt
      181 tttgtaccgc gtaagcagat aactgagcca atatgctgta ttgcgcagaa tcattattca
      241 gcacagttgt cccaatagaa cgtgcagagg tcgtaaatcc atttgccgtt gccgcaactt
      301 ttgaaacaac gttctcaaca aatacatcgg ccagtttagt aactgttttc acgatgtttg
      361 aaccaacttt gttagcgaca tcgccaactt tagacataag tgaagacacc ccgccaatgc
      421 cggctgtcag aatacttgca gcaatagata ctccgccaaa gacttgcgct gctgtcttta
      481 acgcctgcgg ggccttatcg cccattacat caactgcagt ttgcagtgcc attgctgttg
      541 cgctaacggc aacaacagcc cagagtgccg ggttaaaaat agctgcaata gcagttaccg
      601 cgacgcccaa ccaaccaaag acctgaccaa gaattttact tttttgtgct ttctcttcgg
      661 ctttctgttg ttcttcgagc tgctttttaa actccagcgt tttattatcc agcgccttat
      721 tttgcccatc cgtgtagatc tcgttggagt ttttcatact cgcaactttc tgcgctgaag
      781 catccaggga taacagggtg accatcatca tcatggcctg agggtcaacg ttattcacct
      841 gagaaagata aggatagcca gagcttgcat tctgagtgct gcgctgtggt tcggttccct
      901 ctgttcgact cctaacttca ccactaatac caccaaacag tttactcagg acatcctcag
      961 caacagaggg cgtcactaat gagtggcctg ccggtggcgt tggtaaagcg gtactttctg
     1021 tccaacttgc tgatggggcc gtggatttaa ccagttgtaa atccagcgcg gaagtcactt
     1081 caggctgatt aatacctgaa gtagccgtag cagcgctggc cccagacctc atagactgga
     1141 tatcgctatt tacattaagc atagttatct ccggttattt accaagggat attgctgaaa
     1201 tagttctgta ttgcagtgac tgaatatcag aacgtgcact cgttaatagg tttaacgtat
     1261 ttgacatttg ctgtatttca agctggctat tattcactac cgtggtcaag ttattcgatt
     1321 tggccgaaat agcggccttc accgtttgca aatcaccagc gcctaattca gcatttagat
     1381 cgctaatacc acttactgta atgtcattgc gaggatcatt tatatagtca atcacttctt
     1441 gaggaagttt ggctttctta ttcttgtcag aactactctg aacatcagca attttagcat
     1501 ccacaagatt agccattttt tgggctgtgg ttgactcctt agatgcctca ttcatatcag
     1561 caaactttgc aatcgacaga tcgctttgtg cctgatacat ataggcaaac atgagaagcg
     1621 cagcctgaaa aacaccgact ttattaaata gctgaactac ttcgtctttc gacatactgc
     1681 ctaagtcata gactgtcgat gtcgaagtac tcgcgttcgc actagcaact gatgttgcag
     1741 ttgatgtatc catgtatata cctcttaata atctttctta gcaagaccaa aaacgattaa
     1801 aaaaccaata tcaaataaca tcctccttat aatcaattac tttaccaatc attaataatg
     1861 cattatctct ctgttcccta tcaataaaac aagcgtcagg taatgatgca aaaaagtctt
     1921 tcaataggtg gaaataataa acatgtttat cttcgacaat cgatacccga gaagggcttt
     1981 ccaggagcca gtcttctgtt aaccatacat gttcaataat agacaatact tcatttaaaa
     2041 tga
//
LOCUS       DAZFKM010000330         2026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFKM010000330 DAZFKM010000000
VERSION     DAZFKM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..331)
                     /locus_tag="ACHKAY_005449"
     CDS             complement(<1..331)
                     /locus_tag="ACHKAY_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5703896.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDT"
     gene            complement(420..929)
                     /locus_tag="ACHKAY_005450"
     CDS             complement(420..929)
                     /locus_tag="ACHKAY_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703897.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            1387..1845
                     /locus_tag="ACHKAY_005451"
     CDS             1387..1845
                     /locus_tag="ACHKAY_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703898.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            1945..2026
                     /locus_tag="ACHKAY_005452"
                     /pseudo
     CDS             1945..2026
                     /locus_tag="ACHKAY_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          633 a          455 c          386 g          552 t
ORIGIN      
        1 atgtatccgg cacaccagaa aggctggcct tcaggctgta cacgcagcca aaccgtttat
       61 cgttaaacac cacatttttc tggctgatgc cccattcttc acgcagcgcg gcaatcaatt
      121 gcggtgtacg ggtaagcagc aagcgaaaag gcagttcaaa actggtgacg taatccacat
      181 tcagcagtgc aatacgcaac cgttcttctg gcccggcctc tgtctgccgg cactcctcca
      241 gaacatcctg ccactgccgg cgaagacggg gggattcgtt ccgttctgta aaacagtatt
      301 tatccgccag atagtcaatt cgtgtacgca tactgaaagg aattccgtat aaagattcag
      361 cagacaaaac tttatcagcc agtaaaaatt aacggaagca ttgatattcc tcccgacaat
      421 caccggatga ttgttgcaat aacctgcgac ataaaggact gaacagccgt ttttaacgcc
      481 acgtattgcc ctgatgaacc aggccgggcc acccgaaaac atctttccac ccggcattgt
      541 ccgccagcac ttatcaccga ccaggtctgt tgcagtaaat ccaccagttg cgcacattct
      601 tcatcagcca tgacgccgac tcgcggatgg caaatactcg tctggctgag cagcaacaga
      661 catcgtatct gtgctccttc atgttgttcc cgcaaccagc gtaataccac cggcgctgat
      721 gggctgaccg tgtgattatg ctccgtaata cgttcgtgta cccgtcggtg aaaggaacta
      781 caaaatgtct gaatctgttg accatagatt ccttctgccg aatagagcgt gaagtgaaat
      841 gccaacagat ttggccagtg gtccaccgcc tgctgaacaa aacgccggat ttcccccggc
      901 tctgaaatta aggcttcggt tatttgcacg aaatgttcct tttcaacccc tgttgaattt
      961 tgtcaagccg gtgcagacac agtaaagcgc gcatcaacac ctgaaaggta cgatacaaac
     1021 acaagttaca aacaataaga acggaaggac aattaatcag tgaggaataa taactaaatt
     1081 aaccaaaaga atgttactga caatactacc aatacttaaa tagcaatata ttaattaatg
     1141 taagaacaga tgcccggtag gacatataca caatacatcc acaaacacac tcttttcatt
     1201 aaatcaatca cctgattaaa ccatgaaagt gtgatgtaag tcaaaattac tgatactatc
     1261 aattgataat gattatctct atcattatcg tgaaattcga gaatgaaagg agaagtagtc
     1321 attataagcc ttttataaat cgtcatcatc atagtaaagt agaacacttt ttatatgagt
     1381 tactatatga aaatatactg gacaagaaaa cgtattcctg aactttcagc acttccacct
     1441 tcattaagaa agaaaaattt tactgacgca tataatgcag catcttctca tatcgaatac
     1501 tggataggtg ctggcgtctc atttatctcg atgatgattt tatccagagt atatgacttt
     1561 cttttacccg cccaagatac tttcccgggt gatatcatca gaagtttatg tgttgtgtgt
     1621 ccatccatat taatctggtt tcagttttcg gtatatgtca tgaggaaata ttacagacat
     1681 attctggtgc gaggaaaaga aaccgaaacc atttcagaaa ggttgatacg tgaggctgat
     1741 acacgagaat atgaactatg gcgtccggtc cgtcgctttt tctccattgt tttcctcatt
     1801 gttctattag gttgcattca ttcactgata acaactataa aataaaagtt atggctgttc
     1861 tgatacagga aaataaacgt tcattctatg gttacctctt cctgttatat agaagaattt
     1921 taaacgacaa gtaagggaat ataaatgaaa ataacaaaaa ataaaaatgc aactataaaa
     1981 aacagtcatc agtcggctgc ttttcaatgt ttaccttatc attttc
//
LOCUS       DAZFKM010000331         1989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFKM010000331 DAZFKM010000000
VERSION     DAZFKM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1989)
                     /locus_tag="ACHKAY_005453"
     CDS             complement(<1..>1989)
                     /locus_tag="ACHKAY_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5703899.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFT
                     QLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGK
                     SAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVD"
BASE COUNT          390 a          631 c          524 g          444 t
ORIGIN      
        1 atcaactttt tccagcagct ctttaccgag atgggattcg gttatcttgc ctttgaaaaa
       61 atccaggtaa ccttccgcat catcgctcgc ccgaccgacg ccctccacga atgccgattt
      121 gccaacggtg ttcacactgc ggatataaaa gtaataatca tggcccggtt tgatattgat
      181 actggcggct atccagtaca gcgccgtacc aagatagcgg gctgtggttt caacctgcct
      241 gatatcggta atccgcgttt ccgagaacca gaactcaaac tgtaccgtcg ggtcataaac
      301 ggcaagatgc ggcgtggcgg ttatctgaaa atagcccggc gtcagctcaa tcctcgacgg
      361 tgctgccggt gcggcaatcc ggaacgatac cgacgccgga tcgccctgct gcccccacgc
      421 atttaccgcc cggactgtca gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt
      481 ggtttccgtc gtccgggccg tgctgaccag ccgctcactg ccgtcatccg ctgttacggt
      541 cagacggagc aggaagctca cgcccttcac caccttcggc gtgtcccagc gcgccagcac
      601 ctgatattcc ccgctgtctg cggtgacttc ggcggtcagg tgctgcaccg ctggcggcgt
      661 gacaccattc accgtgccgc tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc
      721 ttctttttcc ggtacatgct gcacggcagt gatggcatac gtgccgtcat cgttatcacg
      781 gatactcaca cagcggaaca ggcgctgacg caacgtcggc agcttcagcc cccacacact
      841 gtattctgca acgccgtcag gaacccggtt cactttcacc ttaagtccgt cggtgacgga
      901 ctgaacctcc acgctgaccg gattaccact tccgtcaacc aggcttatca gcgtggtacc
      961 ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc
     1021 cagcacgcga ccaccggtgc tgataccggc atagtcatca tcgaagattt caataacatc
     1081 gcccggtaca tggcgaagcc cttcagcacc cacgctgaaa tccacggtct gcgtctccag
     1141 cagttccgtt ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc tggtacagcc
     1201 aaaggcatcc atcttcgtga cattacgacc gtaacgggca atggcctgcg tatcttcaac
     1261 aagctctgtc gccgtctccc agccgttgtt cgggtcaatc cagttcacct caacggcatt
     1321 atggcggtcc ttcagggcgc tgaagctgta gcggaacggc gcgccatcat ccggcatcac
     1381 cacattactg cggttatagg tccacacctt atctgatggt cggtcctgca cgaacgtcag
     1441 cgtctgcccg ttccataccg gcatacagcg catcgccgag cagaaatcac tgagcacatc
     1501 ccacgccttg cgctgtgtgg tcaggtatgc attacaggtg atgcgcggct ccgtgccgcc
     1561 aaagccgtcc ggtactgact ggtcgcagta ctggccgatg acatacagcg cccatttgtc
     1621 cacatccgcc gcaccaagac gtttccccat gccgtagcgt ggatgggtca gcatatccca
     1681 cagacaccag gccatgttgt tgctgtatgc cggtttaaac gttccgtccc agataccgct
     1741 gtattgtcgc gtctgcgggt tatagttcga cggcacctgc agaatgcgcc cgcgaagatg
     1801 ataattacga ctcacctgct ggctgccgaa ctgctccaag tccacctgta cgccgaccag
     1861 tgccgtgttc gggtagcact gtttcacatc gatgatttcg gtgtatgacg accagagcgt
     1921 tttgttctgc agctggtctg tggtgctgtc cggcgtcatc ctgcgcatcc ggatattaaa
     1981 cgggcgcgg
//
LOCUS       DAZFKM010000332         1988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFKM010000332 DAZFKM010000000
VERSION     DAZFKM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(310..582)
                     /locus_tag="ACHKAY_005454"
     CDS             complement(310..582)
                     /locus_tag="ACHKAY_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703900.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            742..1275
                     /locus_tag="ACHKAY_005455"
     CDS             742..1275
                     /locus_tag="ACHKAY_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5703901.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1496..1609
                     /locus_tag="ACHKAY_005456"
     CDS             1496..1609
                     /locus_tag="ACHKAY_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703902.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1611..1928
                     /locus_tag="ACHKAY_005457"
                     /pseudo
     CDS             1611..1928
                     /locus_tag="ACHKAY_005457"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            1873..>1988
                     /locus_tag="ACHKAY_005458"
     CDS             1873..>1988
                     /locus_tag="ACHKAY_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO5703903.1"
                     /translation="MVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQTPLNG"
BASE COUNT          525 a          403 c          540 g          520 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat
      421 attatgtttc agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt
      481 tttatgtgag atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc
      541 tgccatcaga agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct
      601 gcgcaaactt tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc
      661 aagggatatg tttatgagag cgaccatgag tgttggatta tatatctaac atatcaaggg
      721 attagaaatc gataaatccc catgaacgaa aaaataaaat acggcctgtc ggctgccgtt
      781 ctggcgctga ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa
      841 aaggaaggta accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt
      901 ggtgccattc tggtggatgg taagcctgtt attcctggca tgaagctgtc aaaggaaaaa
      961 tgcgaccggg ttaatgccat cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc
     1021 aaagtgccgc tgaccgaacc ccagaaagcg gggatcgcgt cattctgtcc gtataacatt
     1081 ggccccggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg tgatcgaaaa
     1141 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
     1201 tcaaataatt gctacggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
     1261 gacatcgaca gatagcagaa tattttcctg aaaaatgacg ttggccaacg cgggtggata
     1321 acacgaaatc ctgaaaactg gtaaaaccta agtgaataaa agtaaaaacc ccgtttgttg
     1381 gcagcaagcg gggttttgtg ttttctgacc ttgagtaagg caagggagaa attatgggta
     1441 gggaggtact ttccctgtga ggaagtataa aagattcttt ctgaggttgt ccattatgaa
     1501 aggcattgaa gtggagacgc cagccagtct ggatttaaca agagcggcag cttttgccat
     1561 tcgtattgtg gccattgctg ttctggtctg ggcaatccgt tggtggtgat atgaaccgtg
     1621 ttctgtgcgt ggttatcatt gtcctgctgg tggcctgtgg tgcgcttagt ctggggctga
     1681 atcattaccg tgatcacgcc atcatctaca aagagcagcg cgataaaaaa gccagtgagc
     1741 tggagctggc gaacgcgaca attactgata tgcagatacg ccagcgtgat gtcgctgcac
     1801 ttgatgccag atactcgagg gaattagccg atgcgagagc tgaaaatgaa actctgcgtg
     1861 ctgatgttgc cgctggtcgt aagcgcctgc ggatcaacgc cacctgctcc ggtaccgtgc
     1921 gtgaagccac cggcacctcc ggcgtggata atgcaaccgg cccccgactg gcagacaccg
     1981 ctgaacgg
//
LOCUS       DAZFKM010000333         1975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFKM010000333 DAZFKM010000000
VERSION     DAZFKM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..>1975)
                     /locus_tag="ACHKAY_005459"
     CDS             complement(32..>1975)
                     /locus_tag="ACHKAY_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5703904.1"
                     /translation="GERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWL
                     TYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQ
                     HLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATD
                     PAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDE
                     RTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSR
                     KPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKL
                     QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQ
                     QHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQ
                     LLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHA
                     GIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVG
                     IGAGVDASVMTLACRTWQECWVN"
BASE COUNT          366 a          633 c          494 g          482 t
ORIGIN      
        1 aaaaatatat attttttcat atttcctcct gttaattgac ccagcattct tgccacgttc
       61 tacaagccag agtcataact gaagcgtcaa cacctgcacc gataccaaca gattctgtcg
      121 atgattctgg attatcaaca tccactacat aagtatacgc aaaatgcccc ccaactccgc
      181 cgccaaccgt tacacctact gaattgctga ctcccgatgc aggtaaagac ttggtctcag
      241 aatatgtgat agccgcattt atcccaccac caattcctgc atgtccgcat gctgtagcat
      301 aaatacaaat atcagataac atttcacctt tctttttagc aaacgcaacg ccaagtcctg
      361 catgaccacc taatgatatt agatctccac tgccagtaat atcaattgat gatgttccat
      421 atgaatcatc atgccaaaca tcatataata attgatataa attatacaaa cccaaaggat
      481 ccatccttat tatcggattc agtgggtaat tatacaggtt ccatcccccc gccagcccaa
      541 tggggtcttg tgtaatatac ctcccctgta gcgggtcata gtaccggttc cggttatagt
      601 acagccctga ttcctcatca tgctgctgcc ccggcagacg gtatggctgg tgcaggtaat
      661 acgggttctc ttcattaagc tggttgcccc attcatcata ctccccgcgc cacgccgtat
      721 tgccgtcttc gctgatgagc gccagcggca ggccccggtg gtcgcagtgg taaaaatgaa
      781 cttttcgcgc cggtgtgtat tccggctcca cctgtctggc cagttgctcc accgtcagcc
      841 cgcactgcgc aagccacgcc cggctttcac tgctcacgcg gtctgcccgg atttcttcct
      901 ccagcctgtc cagcagccgc accagttcag ccggaaatac cacgccgtgc ccgtcctcgc
      961 tcccttcctg ctgcagcttc tccgccaggc tgcggcgctg cgctttctcc cgctcgccgt
     1021 tgtctgtttc gatgcggatg agcggcgcga agctccccgg ctgatatacc gtctggatac
     1081 gcgtggtgtc ggtctgtacc gtcgtcagcc ggtcgccgtc ccacccgtac cacgtcatct
     1141 ccggtttacg cgacagcgac atccaccccg tcaggtcacg ctcccgccgc catacccgtt
     1201 tcgccattcg ccgtcccagc gggtcgtaga ggtagcggct ctcgaccagt ggctcgccat
     1261 gctgtatccg cgtgtagaac accagacggt gctggctgtc gtagtggtag tggtgggtcc
     1321 gctcgtcgtc cgtccggata acacccgccg ggataaggtc cgtcttctcc gtcagtctgc
     1381 cgtattcatc atagtggtaa acatagtgcg catcctctgc gatgcggtta tccggccacg
     1441 ccgtgagggt actgtccggg tgcagctccg ggtccggcag gcggtttccc gccgggtcgg
     1501 tggcatacgg gatgcggatg tccaggtccg gtgcgagggt acgcacgctc tccagcctgc
     1561 ccgtggcgct gtagccgtat tcccgcgtct gtcgcgggcc gctgatgcgc accaggtcgc
     1621 cgttgtcatt ccacccgtag tcacggtcat acaccaggct gttcaggtgc tggctctgta
     1681 actggcctgc gggggtgtat gtgctggtca gtttatatgc ggcattactg cctgccatgc
     1741 tgccaaagct gcgcaccgtc tcccggtgca tgcggtcgcg cgtgtactcc agcagcggcg
     1801 tgtcgccaag cttcatgccc gccagataac cgctgccgta ggtcagccac tccaccggcg
     1861 gcaggctgtc cggcgtgacg cggtttgcca gcccctgttc gttgtaggcg tgtgttgtct
     1921 catgctgcca cagcagttcc cccgtctccg ggttctccac cgtctgccgt tcgcc
//
LOCUS       DAZFKM010000334         1964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFKM010000334 DAZFKM010000000
VERSION     DAZFKM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1230
                     /locus_tag="ACHKAY_005460"
     CDS             <1..1230
                     /locus_tag="ACHKAY_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5703905.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHKAY_005461"
     CDS             1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHKAY_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO5703906.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          590 a          425 c          492 g          457 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccgcc tgaccacaat acagaacgac agaacccgca tccagacgat ttatcagccg
      181 gggagcttca cgccactcat cagggttgaa accgccaccg gtgagcaggc gaaaacgcag
      241 cgccgcagcc tggcggatac ccttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1681 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1741 cttacgaatg tttaataaag atacagcatg aactaattcg tagtgaagag aaataaaatc
     1801 tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg aataggtgtt
     1861 tattttgcgt tccatttgca gggaaagatc acgtaacgct acttttttgt actaaataat
     1921 tcgaatttta agttcaacaa ttgagatact acttattgtc taaa
//
LOCUS       DAZFKM010000335         1941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFKM010000335 DAZFKM010000000
VERSION     DAZFKM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..202
                     /locus_tag="ACHKAY_005462"
                     /pseudo
     CDS             1..202
                     /locus_tag="ACHKAY_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            331..576
                     /locus_tag="ACHKAY_005463"
     CDS             331..576
                     /locus_tag="ACHKAY_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703907.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            548..958
                     /locus_tag="ACHKAY_005464"
     CDS             548..958
                     /locus_tag="ACHKAY_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5703908.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            955..1131
                     /locus_tag="ACHKAY_005465"
     CDS             955..1131
                     /locus_tag="ACHKAY_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5703909.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            1134..1535
                     /locus_tag="ACHKAY_005466"
     CDS             1134..1535
                     /locus_tag="ACHKAY_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703910.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            1495..1704
                     /locus_tag="ACHKAY_005467"
     CDS             1495..1704
                     /locus_tag="ACHKAY_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO5703911.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEYCCAELMSDPNSSMYEEEDDE"
     gene            1697..>1941
                     /locus_tag="ACHKAY_005468"
     CDS             1697..>1941
                     /locus_tag="ACHKAY_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5703912.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQ"
BASE COUNT          612 a          386 c          490 g          453 t
ORIGIN      
        1 tgagtttatg ctcgtcgcac tcaaatgcgt tggcgtttga tggattcttc tgacgttatt
       61 tattgttctg catagctaca ttcgtcttgt gaatgacggt aaagacccat ggtatacgtt
      121 gtttggcgct gcatttgtct gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaactgaggg taagtatcga tggacgaatc aagaaagcag
      241 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
      301 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      361 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      421 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      481 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      541 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      601 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      661 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      721 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      781 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      841 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      901 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      961 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
     1021 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
     1081 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatgcttt
     1141 taattcaacc tggatttgga cttagcatca aaaaaggcca catgtttggc gagaaagagt
     1201 cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg ctaaacaggg
     1261 aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct gactggtttg
     1321 tgaaaaacca ccacgcagtt cgtcaggcaa agagaaaggc caatatgaca tacatgaagg
     1381 cgtatcaaaa agcatggaaa gaacaccgcg atcgatacca acaagacatg gaaaagcttg
     1441 aatcagaaaa catggaatta agacgaaagc tcggtgaagc aaaacgagac attgatgctt
     1501 acaagcgact ttttaatggt gaaagccatg cttagcccat cccaatccct tcaataccag
     1561 aaagaaagcg tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct gcatgtgctg
     1621 gaagttcacg tgtgtgagta ctgctgcgca gaactgatga gcgatccgaa tagctcaatg
     1681 tacgaggaag aagacgatga atgagttaat aaatggcaat gccatcaaaa tgacaagcat
     1741 tgaaatagct gagttggtgg gtaagcgtca tgacaatgtg aaacgtacca tcgaaacgct
     1801 ggctaaaaat ggtgttatcc ggcttcctca aattgaggtt tccgaaagaa tcaataactt
     1861 agggttcaat gttcagtacg agcattacgt cttcgagggc gaacaaggta agcgagacag
     1921 tattgtcgtt gttgcccagt t
//
LOCUS       DAZFKM010000336         1895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFKM010000336 DAZFKM010000000
VERSION     DAZFKM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..710
                     /gene="tnpA"
                     /locus_tag="ACHKAY_005469"
     CDS             33..710
                     /gene="tnpA"
                     /locus_tag="ACHKAY_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO5703913.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKAY_005470"
     CDS             710..1057
                     /gene="tnpB"
                     /locus_tag="ACHKAY_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5703914.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1077..>1895
                     /locus_tag="ACHKAY_005471"
     CDS             1077..>1895
                     /locus_tag="ACHKAY_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO5703915.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTR"
BASE COUNT          448 a          530 c          512 g          405 t
ORIGIN      
        1 tctggtctgt ttcctccggc tctacaaaaa taatgtccat catttttaat ggacactatc
       61 gtatgaaaca ccggacctgg atcactgaag ctttacgtct tcactttgaa gaacatttac
      121 cccgggttgt ggccgggcgt cgcctgggtg taccaaaatc aacagtttgt agtatgttcg
      181 tgcgctttcg gagagctggc ctttcgtggc ctttgcccgc aggcatgtcg gagcaggaac
      241 ttgatgcctg cctttacgga caattttcca cggtaccagt cgtacgtcct gaaagcaccg
      301 ttatatccga agcccccgtg gtaaaaaaac gtccccggcg gcccaacttc ccttatgagt
      361 ttaaaatcgc cttagtggag cagtcactgc agcccggagc ctgtgtggcg cagatcgccc
      421 gggaaaacgg aatcaacgat aacctgctct tcaactggcg ccatcaatac cggaaaggtg
      481 gcctgctgcc ttccggaaaa aatatgccgg cactgcttcc cgtgacgtta acgccggagc
      541 cggataataa aatcccggcc cccgcacagg aaccagagca gataaataca ccgtccgaca
      601 gtctgtgttg tgagctggtt ctgccggccg gaactctcag gcttaaaggt aaactgacgc
      661 cggcgttatt acagacactt atccgcgaaa taaaagggag cagccactga tgatatctct
      721 ccctgcaggt tcgcgtatct ggctggttgc aggtatcacc gatatgcgaa atggctttaa
      781 cggcctggca tcaaaagttc agaacgtcct gaaggatgac ccgttctccg gacacctgtt
      841 catcttccgc ggacgccggg gtgaccagat aaaagtgttg tgggctgaca gtgacggact
      901 gtgcctcttc accaaacgcc tggagcgggg ccgcttcgtc tggccagtca cccgtgacgg
      961 caaggtgcac cttactccgg ctcagttatc catgcttctt gaaggtatca actggaagca
     1021 cccgaaacga acggaacgcg ctggaatccg catataaccc gttgtaaagt gaggatatgg
     1081 acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg caacaggaca
     1141 ccatccgcca gatggctgaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt
     1201 ccgaaatcaa ccggctgaag gcgctggttg cgaaactgca acgtatgcag ttcggtaaaa
     1261 gctcagaaaa acttcgtgca aaaaccgaac ggcagataca ggaagcacag gagcgaatca
     1321 gcgcacttca ggaagaaatg gcggaaacgc tgggtgagca atatgacccg gtactgccat
     1381 ccgccctgcg ccagtcttca gcccgtaaac cgttaccggc ctcacttccc cgtgaaaccc
     1441 gggttatccg gccggaagag gaatgctgtc ctgcctgtgg tggtgaactc agttctctgg
     1501 gatgtgatgt gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac
     1561 aacgtccgaa acaggcctgt tgccggtgcg accatatcgt gcaggcacca gtaccttcaa
     1621 aacccattgc acgcagttat gccggagcgg ggcttctggc ccatgttgtc accgggaaat
     1681 atgcagacca tctgccgtta taccgccagt cagaaatata ccgtcgtcag ggagtggagc
     1741 tgagccgtgc cacactgggg cgctggacag gtgctgttgc tgaactgctg gagccgctgt
     1801 atgacgtcct gcgccagtat gtgctgatgc ccggtaaagt ccatgctgat gatatccccg
     1861 tcccggtcca ggagccgggc agcggtaaaa cccgg
//
LOCUS       DAZFKM010000337         1893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFKM010000337 DAZFKM010000000
VERSION     DAZFKM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..1830
                     /locus_tag="ACHKAY_005472"
                     /pseudo
     CDS             58..1830
                     /locus_tag="ACHKAY_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          713 a          355 c          295 g          530 t
ORIGIN      
        1 tgcaaaatat ttttatcaat agttagtatt atggaaacca ttaattcagg aggaaacttg
       61 attccaaatt caacttcaaa taaaggttat gtatgcattg acatgcagtg ttcgtcaacc
      121 tctgaaccaa cagcttctac ctccagcaac cggagtatta aattagccgc ttccacaaat
      181 gtatatccga taacaagaaa cgactccgag ctcactctga acgattttct tgataatagc
      241 tcttctacgt catcattgga ctacattaat gaattgggtt ctcaactgac gttaaatgat
      301 tttcttgaca acataaagac aaatgaggtg gatagaacat gtacggatgt ggtaattaat
      361 atcccacaag agatacaaac aaatacacag gaaaatgatt tgttattatc cgataaaaat
      421 aattcaatat gcattgaaat cgatgaaaga attacaaaaa tcctaacatg caagcaaaaa
      481 tatcaactgg acagcatcat tcatgaaatt ataccaaaag agaatgagag tgcagaaact
      541 gttctccatc ttatgagagt tctgaacgat caatatcatc aggtatataa tcagtcagga
      601 tgtttttata aagcctatat ggccatacac aataaaatcg aacagatact tccatatgcg
      661 ttcagagccg gaggcggaat cagcattcac ttgctcatac aggcattatt ttttaatggc
      721 gactataaca aatcaccttc acagtctcaa caaccatctt tatatacatc accttctcca
      781 acaataaata cagaagcatt cttaagtaat gtattatcac tagatataac ccaggtacgc
      841 atacttggtg atttactatc agcaacttta tttcatgcac caacaatatt ctatcaatat
      901 tctaaactaa tagatgaagt taagtattgt ataagtaata aaaaaataac aggttcggtt
      961 atagcacgat ttactctatg tttaacaagt acattactaa ccatgtcacc actgttaatg
     1021 cttaatggag cagttaaaac aggtagcata gtaagaacta taggtagggg agtgagttat
     1081 gttgatatac cattggcctt agctatatta ggtgactcgt ggtataaagc ttataaacat
     1141 ggttcttctg ataacccaaa ttctgctcag agatttatat cgcaagaagc ggcctttaaa
     1201 accacccagc gggtattaac acaaggatta agtctgatgt cctctttatc gggagcaatc
     1261 atgcgctctc ttgagaaagg cacaccacca caaatgatgt ctttattcat cgtaaacata
     1321 ctaaatctat tatttcatca aaatccatat gaaggggcat cagcaagtgc taatgctttg
     1381 aaaagatcaa cttactccca taatccggac atactaaata ctcaggcaat agctctttgt
     1441 gttgacctcc agcatacaaa aaatataacc atgccacttt tcaaaacaaa agacaggata
     1501 tcttacgcat tcaatggaca aagaacatcc ccagaagacc aaaaacaaat actgaaagaa
     1561 gttataaact cctgtaccca aggagaaaga gccattttaa atacatcaca atcagaaaca
     1621 tgcaaacata aaatcgatga gatttatgaa aaaagattct cagaaacaga actaaataca
     1681 taaccaaacg aaatgaaaaa tttcttgata tttttaaaca aaactcatga aaaagatatt
     1741 tcgcgtttaa gcatgggtaa cgaagttaat gaaaaaataa ttgcagttat agtcaagacg
     1801 ttagcatata gagagtctat gttgtgttag actttaactc tatattgata taacattagc
     1861 caaatattca acagtgtatg cgaccaaaca cca
//
LOCUS       DAZFKM010000338         1890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFKM010000338 DAZFKM010000000
VERSION     DAZFKM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            152..823
                     /gene="queE"
                     /locus_tag="ACHKAY_005473"
     CDS             152..823
                     /gene="queE"
                     /locus_tag="ACHKAY_005473"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO5703916.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1163..1740
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1167..1191
                     /rpt_unit_seq="tccccgcgccagcggggataaaccg"
BASE COUNT          483 a          446 c          463 g          498 t
ORIGIN      
        1 tttcttctga cataatacgt tactcccttt aattattaac gatatgtttc gcatttttct
       61 ttaaatctgc ctgtaacaga atgttaataa gacatgattg ttgtggcagg atctgcaata
      121 attaacaatt atccacttca cagagaatgc tatgcagtac ccgattaacg agatgttcca
      181 gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc atttttattc gtttacaggg
      241 atgcccggtt ggctgtgcct ggtgcgacac caaacacacc tgggaaaagc ttgaggatcg
      301 ggaagtctcc cttttcagca ttctggcgaa gaccaaagag agtgataagt ggggggcggc
      361 gagcagtgaa gatttgctgg ctgtcattgg tcggcaggga tataccgcgc ggcatgtggt
      421 gattacgggt ggtgagcctt gcattcatga tttgctgcca ctgactgacc tgctcgaaaa
      481 gaacggtttt agctgccaga tcgaaaccag tggaactcat gaagtacgct gcacaccgaa
      541 tacctgggtt accgtatcgc caaagctgaa catgcgcggc ggctatgacg tgttgtcaca
      601 ggcactggag cgagccaacg aaatcaagca tccagtgggg cgcgtacgtg atattgaagc
      661 actggatgaa ctactggcaa cgctgaccga tgataaaccg cgagtcattg cactgcagcc
      721 gattagccaa aaggatgatg ccacacgttt gtgcattgaa acctgcattg cgcgtaattg
      781 gcgtttgtcg atgcaaacac ataaatatct aaatattgcc tgattaaaca tttataagcg
      841 ttataaatgg gtggaacctg taaagacttc tactcattta tattgtttgt cgcctctgaa
      901 aactcctcca ttttacccat ccagggctaa tcattagcat tctctacaaa ttctgtggca
      961 ttaatttttc gctggagtga aaattattgc ggtaaagttt ggtagatttt agtttgtata
     1021 gagttatttt aaatatttac ctttttaatc aatggattaa gtgctcttta acataatgga
     1081 tgtgttgttt gtgtgatact ataaagttgg tagattgtga ctggcttaaa aaatcattaa
     1141 ttaataatag gttatgttta gagtgttccc cgcgccagcg gggataaacc gatctcctca
     1201 tcctcccagc taccgacagt agtgtgttcc ccgcgccagc ggggataaac cgcaagatct
     1261 gccgcgagtg gtttcacccg gcaggtgttc cccgcgccag cggggataaa ccggatattt
     1321 gggcgtaata ccgcccggat agtgtgtgtt ccccgcgcca gcggggataa accgtggtac
     1381 ggaacatgct gtctatattt cgaccagtgt tccccgcgcc agcggggata aaccgccagt
     1441 ggagccgccg cgaatttaca cacgattgtg ttccccgcgc cagcggggat aaaccgtaag
     1501 gccgtcgccg gatcagcctg gctatgccga gctccccgcg ccagcgggga taaaccgttc
     1561 ttgcgggtgt tgcaaatatt cttcacgtag agctccccgc gccagcgggg ataaaccgga
     1621 gcctgacgag actactgagg ccgttctgtc gagttccccg cgccagcggg gataaaccgg
     1681 acgccgccgc cgcgaagccg tttccgatgt tgagctcccc gcgccagcgg ggataaacca
     1741 ccatataacc cgttatctct ttctcaagtt tttatattag cagtacttgt aataagcaac
     1801 atatccacgt aacacctcat gttcaaaata gttctccatg ccagagaggt tcacaattat
     1861 cgatacaaaa aattaaattt aatcaaagtg
//
LOCUS       DAZFKM010000339         1883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFKM010000339 DAZFKM010000000
VERSION     DAZFKM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1883
                     /locus_tag="ACHKAY_005474"
     CDS             <1..>1883
                     /locus_tag="ACHKAY_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO5703917.1"
                     /translation="FPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEALRRFELMV
                     EEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAGQAASSAQS
                     ASSSAGTASTKATEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQSAATSASTAT
                     TKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     EATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEA
                     TRQASAAKSSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLS
                     SATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAETP
                     KAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATV
                     TMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTN
                     FAWV"
BASE COUNT          502 a          534 c          551 g          296 t
ORIGIN      
        1 ttcccgccgt cacatgccgg gaccatcacc gtgtatgaag attcccgacc cggtacgctg
       61 aatgattttc tcggtgccat gacggaggat gatgcccgtc ctgaggcact gcgccgtttt
      121 gaactgatgg tggaagaggt ggcgcgtaac gcgtccgcgg tggcacagaa cacggcagcc
      181 gcgaagaagt cagccagcga tgccagcaca tcagcccgtg aggcggcaac ccatgcgact
      241 gatgctgcag gctcagcacg cgcagccagc acgtcagccg gacaggccgc gtcgtcggct
      301 cagtcagcgt cttccagcgc aggaacggca tcaacaaagg ccactgaagc atcaaaaagt
      361 gctgccgctg cagagtcctc aaaaagcgcg gcggccacca gtgccgctgc ggcgaaaacg
      421 tcagaaacga atgcggcagc gtcacaacaa tcagcagcca cttctgcatc caccgcgacc
      481 acgaaagcgt cagaagctgc cacctcagcc cgggatgcgt cggcttcaaa agaagcggca
      541 aaatcatcag aaacgaacgc atcatcaagc gccagtagtg ccgcttcctc ggcaacggcg
      601 gcaggaaatt ccgcgaaggc ggcaaaaacg tccgagacga acgctaagtc ttctgagacg
      661 gcagcgggac agagcgcctc agctgcggca ggctcaaaaa cagcggctgc gtcgtctgcc
      721 agtgccgcgt caacaagtgc cgggcaggcc tcagccagtg ccaccgccgc cggaaaatcg
      781 gcagaaagcg ccgcatcatc cgcttcaaca gccacaacga aggctggcga agccactgaa
      841 caggccagcg cagcagcgag ttctgcttcc gcagcgaaga catccgaaac gaacgcgaaa
      901 gcgtcggaaa ccagcgcaga atcctcaaaa acggctgccg catcgtcagc cagttcggcg
      961 gcgtcatcgg catcatcggc gtctgcttca aaagatgagg cgaccagaca ggcgtcagca
     1021 gcgaagagca gcgccacgac ggcatccacg aaggcgacag aggcagctgg cagtgcgacg
     1081 gcggcagctc agagcaaaag tacggcggaa tccgcggcaa cgcgcgccga gacagcagct
     1141 aaacgggcag aggatattgc atccgccgtg gcgcttgagg atgcgagcac gacgaaaaag
     1201 gggatagtac agctcagcag tgcgactaac agcacttccg agtcactggc ggcaacgcca
     1261 aaagccgtta aggccgcgta tgacctggct aacgggaaat acaccgcaca ggatgcaacg
     1321 acagcacaga aagggatcgt tcagctcagc agcgcgacca acagcacatc tgaaatgctg
     1381 gcggcaacgc caaagtcggt aaaggcagcc tatgaccttg ctaacgggaa atataccgct
     1441 caggacgcta cgacagcaca aaaaggaatt gtccagctca gtagtgcaac caacagcgca
     1501 tctgaaacgc ttgccgagac accgaaagca gtgaaagcag ctaatgataa tgcgaatggt
     1561 cgggtacctt ctgcccgtaa ggtgaatggt aaggcgcttt cagcggatat aacactgacg
     1621 ccgaaagata ttggtacgct taactcaaca acaatgtcat tcagcggtgg tgctggttgg
     1681 ttcaaattag caacggtaac catgccacag gcgagttctg ttgtttcaat tacgttgatt
     1741 ggtggcgcgg gatttaacgt ggggtcacct caacaggcag gtatatctga acttgttttg
     1801 cgtgcaggta atggtaatcc gaaggggatt actggtgctt tatggcagcg cacatcgaca
     1861 gggtttacaa attttgcctg ggt
//
LOCUS       DAZFKM010000340         1879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFKM010000340 DAZFKM010000000
VERSION     DAZFKM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            243..1190
                     /gene="stxA1a"
                     /locus_tag="ACHKAY_005475"
     CDS             243..1190
                     /gene="stxA1a"
                     /locus_tag="ACHKAY_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5703918.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1200..1469
                     /gene="stxB1a"
                     /locus_tag="ACHKAY_005476"
     CDS             1200..1469
                     /gene="stxB1a"
                     /locus_tag="ACHKAY_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5703919.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          499 a          332 c          462 g          586 t
ORIGIN      
        1 gtggctggct tttttatgtc cgtaacatcc tgtgtatcaa taaatgttgt tatctacgta
       61 cgtcaagtag tcgcatgaga tctgaccaga tatgttaagg ttgcagctct ctttgaatat
      121 gattatcatt ttcattacgt tattgttacg tttatccggt gcgccgtaaa acgccgtcct
      181 tcagggcgtg gaggatgtca agaatatagt tatcgtatgg tgctcaagga gtattgtgta
      241 atatgaaaat aattattttt agagtgctaa cttttttctt tgttatcttt tcagttaatg
      301 tggtggcgaa ggaatttacc ttagacttct cgactgcaaa gacgtatgta gattcgctga
      361 atgtcattcg ctctgcaata ggtactccat tacagactat ttcatcagga ggtacgtctt
      421 tactgatgat tgatagtggc tcaggggata atttgtttgc agttgatgtc agagggatag
      481 atccagagga agggcggttt aataatctac ggcttattgt tgaacgaaat aatttatatg
      541 tgacaggatt tgttaacagg acaaataatg ttttttatcg ctttgctgat ttttcacatg
      601 ttacctttcc aggtacaaca gcggttacat tgtctggtga cagtagctat accacgttac
      661 agcgtgttgc agggatcagt cgtacgggga tgcagataaa tcgccattcg ttgactactt
      721 cttatctgga tttaatgtcg catagtggaa cctcactgac gcagtctgtg gcaagagcga
      781 tgttacggtt tgttactgtg acagctgaag ctttacgttt tcggcaaata cagaggggat
      841 ttcgtacaac actggatgat ctcagtgggc gttcttatgt aatgactgct gaagatgttg
      901 atcttacatt gaactgggga aggttgagta gcgtcctgcc tgactatcat ggacaagact
      961 ctgttcgtgt aggaagaatt tcttttggaa gcattaatgc aattctggga agcgtggcat
     1021 taatactgaa ttgtcatcat catgcatcgc gagttgccag aatggcatct gatgagtttc
     1081 cttctatgtg tccggcagat ggaagagtcc gtgggattac gcacaataaa atattgtggg
     1141 attcatccac tctgggggca attctgatgc gcagaactat tagcagttga gggggtaaaa
     1201 tgaaaaaaac attattaata gctgcatcgc tttcattttt ttcagcaagt gcgctggcga
     1261 cgcctgattg tgtaactgga aaggtggagt atacaaaata taatgatgac gataccttta
     1321 cagttaaagt gggtgataaa gaattattta ccaacagatg gaatcttcag tctcttcttc
     1381 tcagtgcgca aattacgggg atgactgtaa ccattaaaac taatgcctgt cataatggag
     1441 ggggattcag cgaagttatt tttcgttgac tcagaatagc tcagtgaaaa tagcaggcgg
     1501 agattcataa atgttaaata catctcaatt cagtcagttg ttgccggtct gataatagat
     1561 gtgttagaaa atttctgcat ggtgaatccc cctgtgcgga ggggcgactg gtgaacggta
     1621 tgatctcttt gatgatcgta agcgagaata cgcgggtttg gtggcaccag gccgaactca
     1681 ccgggaggca cccggcatca tgctgtatac agagattagg catatatcca ggctcctcat
     1741 cgcaggagcc tttttacatg caaaaaaaag cccgagtggg ttcgggcaac agcatgagat
     1801 acttgcattg tcatttttat cgtgcggatt ttaaccagga ttcataaggc tgcgcaactg
     1861 cgcggccttt ttcgtattt
//
LOCUS       DAZFKM010000341         1869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFKM010000341 DAZFKM010000000
VERSION     DAZFKM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..182
                     /locus_tag="ACHKAY_005477"
     CDS             <1..182
                     /locus_tag="ACHKAY_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703920.1"
                     /translation="GWFPDTDGALITGLTFLDPKDATRVQGLFQHLQVRFGDGPWQDV
                     KGLDEVGSDTGRTGE"
     gene            184..453
                     /locus_tag="ACHKAY_005478"
     CDS             184..453
                     /locus_tag="ACHKAY_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703921.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGWMK"
     gene            663..1310
                     /locus_tag="ACHKAY_005479"
     CDS             663..1310
                     /locus_tag="ACHKAY_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5703922.1"
                     /translation="MPINLTAYLTASGGLGEIPQDTLSGIRILAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(1570..1789)
                     /locus_tag="ACHKAY_005480"
                     /pseudo
     CDS             complement(1570..1789)
                     /locus_tag="ACHKAY_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          536 a          333 c          472 g          528 t
ORIGIN      
        1 atggctggtt cccggataca gatggcgcac tcatcaccgg actgaccttt cttgacccca
       61 aagatgccac acgggttcag gggctgtttc agcatttgca ggtcaggttt ggtgacgggc
      121 cgtggcagga tgttaagggg ctggatgaag tgggcagtga tacaggcaga acaggagaat
      181 gacatgaata tactaaaaaa acttatgcag cgtctgtgtg gttgcggaaa gcatgatgac
      241 cgtgaacacg gggagttact tacagcacaa ctgcgtctgg ggccggcaga catcctggag
      301 tccgatgaga atggcattat cccggagcag gccagggtaa tcacgcaggt ggtgatactg
      361 gatgcggata aaaagcagat acagtgcgtg gtaagaccgc tgcaaattct gcgtgctgac
      421 gggacgtggg aaaatattgg ctggatgaag taacccgaca gcttcaaaaa accggagtct
      481 ggctccggtt ttttgttgtc atgtcatggt gatgtttgtt aatgaagtta aatttatttt
      541 ggcttgataa tggagtctta tcttttgcaa gactgataat ggtgtgtgaa agcattgtga
      601 taagtaagat gcttcaacag gaaatcaagt aaaatgttaa tgaagttaat gggggtttta
      661 ttatgccaat aaatttaaca gcatatctaa cagcgagtgg gggactgggg gaaataccgc
      721 aggatactct ttctggcata cgaatactgg cttttaacgg aggagctcag gttcagatgg
      781 ggagcactat tgttacaata cgttctgtct ttttaggctt ctttatggga agtgtaagcc
      841 ctgaaggatt atcagagaga gctttacgaa ctgcattgaa taatgttaat cgtcttgaac
      901 gcgaccttaa tggagggcta tccggccgcc agattttagc aagtataccc agcccgtatg
      961 cttctccacc tcgtccttct gtaatgcaaa cagagttggt gttagagcaa atagaaaaat
     1021 gctcatttaa tgttaactcg tcatctctga gggcgactga agaggcatta acatgcccga
     1081 ttacattatg catccctgag catggtgtga ttatgagaaa tgctggagat tcagatgtat
     1141 gtactttata tgataaagaa tcattaaaac atcttgttaa cacaagttca cctcatcctc
     1201 tgagcagaga aaaaataaca gagtcaatga ttgttaagga aaactcgtgc tattttgatt
     1261 tcacatcagg aagtattaaa aatgttcgtg gtgagataac tagactataa gatgttagaa
     1321 catattgact gataagtgta attcacagac atcttttcag ttttttctgt taattttcta
     1381 attgatggac tgtatacagg gataaaagga gagggtattg tctggacatt tctgttagca
     1441 taattggaaa tgtgcgtgaa aaaaagcata acagacatca tgtgttatgt atgtattcag
     1501 agtgcccata attcaccagt gcagggataa attgtaaaaa aatcgcagta cgtcgtatgc
     1561 agaacggtgc ggcgtacgca gttccagtgg accgtcgcct gtgataacgg tctttgtgga
     1621 ataaccgttg cgggagttag ctcctgatct ggactgattt ttctcattcc caagatggtg
     1681 tgtcatctct gcattgagag cggcttcaac gctgagcttt ttcagcaacc gatcaaactg
     1741 actgaggtct tctgaggggg ctgaggtttt tggccagttc gttagcaata caccagaaaa
     1801 attctaaacg agcctgtaca tagatttgtg taattgcctg attttgatat gttcaaccca
     1861 acatcaaat
//
LOCUS       DAZFKM010000342         1868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFKM010000342 DAZFKM010000000
VERSION     DAZFKM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(372..448)
                     /locus_tag="ACHKAY_005481"
     tRNA            complement(372..448)
                     /locus_tag="ACHKAY_005481"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Arg,seq:tct)
     gene            complement(462..538)
                     /locus_tag="ACHKAY_005482"
     tRNA            complement(462..538)
                     /locus_tag="ACHKAY_005482"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(502..504),aa:Arg,seq:tcg)
     gene            complement(548..623)
                     /locus_tag="ACHKAY_005483"
     tRNA            complement(548..623)
                     /locus_tag="ACHKAY_005483"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(588..590),aa:Ile,seq:cat)
     gene            complement(664..777)
                     /locus_tag="ACHKAY_005484"
                     /pseudo
     CDS             complement(664..777)
                     /locus_tag="ACHKAY_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(1065..1499)
                     /locus_tag="ACHKAY_005485"
     CDS             complement(1065..1499)
                     /locus_tag="ACHKAY_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5703923.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(1492..1686)
                     /locus_tag="ACHKAY_005486"
     CDS             complement(1492..1686)
                     /locus_tag="ACHKAY_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO5703924.1"
                     /translation="MTFTVKTIPDMLLEAYGNQSEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            complement(1683..>1868)
                     /locus_tag="ACHKAY_005487"
     CDS             complement(1683..>1868)
                     /locus_tag="ACHKAY_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5703925.1"
                     /translation="HKSGNLVPYRVELINRIGQEAVDEIESNHNRHRWTIEECKAIKA
                     GYQQKLKDLRNSRSEAA"
BASE COUNT          458 a          498 c          414 g          498 t
ORIGIN      
        1 aatgacaatg caagtatctc atgctgttgc ccgaacctac tcgggctttt tttgcatgta
       61 aaaaggctcc tgcgatgagg ggcctggata tatgcctaat ctctgtatac agcatgatgc
      121 cgggtgcctc ccggtgaatt ctgcaatgac cagacagaat ccgcaacttg cctatacaat
      181 acgcaaccaa acatctgtca ttatgccccg ccgcccaggg ggattcatca tgcaggattt
      241 ttttaacaaa cgctcagcat gtcaggcaac agtcgactac ctgaattgtg aggcatttaa
      301 catttcactg tccggtgtct ttcctgtaat aaaaagcccg caaaagagag tcagggcaga
      361 taagtgtggt gtggcgcgtt gtactggatt cgaaccagtg accgattgct tagaaggcaa
      421 ttgctctgtc cggctgagct aacaacgcat gatgctgata atggaccgcc atcggggact
      481 tgaaccccgc acagccagct tcgaaggctg acgctctatc ccgatgagct aatggcggta
      541 tgtgatatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
      601 tctcaccact gagctaaagg gccgggcgca ggataataac ggtacgtaac taatcctgca
      661 atatcatccg ttctgactga ctaaatcctg aacttccctg accgtctgct caaaacgttc
      721 agtctccagc tcaacgccaa ttgcacgacg ccccagcgac attgctgctt tgacgctaca
      781 gacataaaaa agccagccac tgggggaggc tggcaaactc gtagagcaaa atgctgttac
      841 gcaaacttcg ttacagggtc atcctgcaat acaaaaaata cacaatattt agaaaactaa
      901 tagtgccatg tgcaattttt aagattttgt tattaattgt ggtcgcacct tcctttctgt
      961 gtactttccg tatagctcac aggattctgg gtacaaaaaa acccgcgcat cggcgggtta
     1021 agcagcgtgg caatgtaacc actcttatca tgatatgcag atttttacga tcgtaaacta
     1081 ttttttcgct gataaaatac agaggttctc cctcccggca attcacgctc aacataccga
     1141 tccatctcaa gcctcactcc cagcatcatc agcatgcctt caacaatccc ctccgctttg
     1201 tgaaggcgtt tacctataca ggtgtcagag cacccatgtt tccgtgccag cgccatgaac
     1261 gtctccccca acacgtaata atcaaccagc aagtcatgca gatcgctgtt gttcctgtta
     1321 aggcgagcca tacacccgca tataatcatc gcgtcatcgt cacaacactg tggacgtgat
     1381 tttacttttt cggggatcag tcccttaaat ccggcagcaa tgggcgacca tgtaacatcc
     1441 tcatggttat ttgccgccca tgccccccag cgctcaagaa cctgccggat atcacgcatc
     1501 agtatcttta ccccatccgc gatgaaccat aaggacgcca ttgacgacgg cgtgcttttt
     1561 cccttcttta tcgccaatgt attttctgac tgtggcacga ttgcagttca gtattcgggc
     1621 tacctcggac tgatttccat atgcctcaag gagcatgtca ggaatggttt ttacggtgaa
     1681 cgtcatgcgg cctcacttct gctgtttcgc aggtctttga gtttctgttg gtaccctgcc
     1741 ttgatcgcct tgcactcttc gatagtccag cgatggcggt tatggtttga ttcgatttcg
     1801 tctactgctt cctgcccgat gcggttaatc agttcgacgc gatacggaac gagatttccg
     1861 cttttatg
//
LOCUS       DAZFKM010000343         1865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFKM010000343 DAZFKM010000000
VERSION     DAZFKM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            632..922
                     /locus_tag="ACHKAY_005488"
     CDS             632..922
                     /locus_tag="ACHKAY_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5703926.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            946..1197
                     /locus_tag="ACHKAY_005489"
     CDS             946..1197
                     /locus_tag="ACHKAY_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO5703927.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1245..>1865)
                     /gene="traT"
                     /locus_tag="ACHKAY_005490"
     CDS             complement(1245..>1865)
                     /gene="traT"
                     /locus_tag="ACHKAY_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO5703928.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          478 a          384 c          435 g          568 t
ORIGIN      
        1 cattaacagg attttgtaat aagccacatt tattctttcg ggaattaaaa aacataacga
       61 aattttgcct cattctgcat ttcattgaaa tagtatgttt ctcatcgaaa gcaagtttgt
      121 caatataaat ttgtaacaga aatgactttc cggggataaa caaaactgtt catggcaatg
      181 gctttctctg tcgccaacat ggatacattg ttccggataa agcactgatg tcattgactg
      241 acgcagacta tcaggccatc tgatgctttc ttttccttca ccggtattga tgatatgcct
      301 tatgaagggg gacttattcc tgtaacccaa tcacaggcac tgtgattact ggtttatgat
      361 tgatgctgat ttgagcagat atgcacagca gttgtggatt caggggatat tttctgccag
      421 atattcatac cagattgatg acctctttgc tcagatgctc ttcgttctga gccagccagg
      481 attttgtggc cacatacatt gttcgccgat ggggaatcga gacaggcgat tggctgtgat
      541 gcgattttgc cgctaactga ggatgagtcc gcccctcccg gggaattcct gtaagttaag
      601 ccgtacctgt tgattagcac taaactgggt aatgaattta tctgccagta tgaatgatgc
      661 cggggacggt tttgaactcc tgatgtcggg taatatgcag gattttaata tttgttgtcg
      721 tcgggataat cattatcctg aacataaact gataccttta tcagagttat ttgacgttaa
      781 ttatggactg aatcttgagc tgaataaact ggagaaagat tcttcaggaa ttaattttgt
      841 ttcaagaacg tccaaaaata atggcgtctc cgccagggtt aaattaaaag atggtattag
      901 tccatgccag cagctgtgtt aactgtagca gcaggaggtt ctgttatgga atcgtttgta
      961 caggattcac cattttatag tggaagagat ctttattggt tacgtcctaa agttgaactg
     1021 actctggaag agaaattgta ctactgctct tgtataagaa gaaacagaca caaatacagc
     1081 tacgggcgtc aggcaaaccg aacgctgaag aatttactgg taccgtcatt agattctgtt
     1141 ccggcatggg tatatggtgt gacagggaaa ataatatctg aattgtctga acgttgagac
     1201 taagagggcc ggaggccagg tgaatggcca ccgggatatt tatatcagag tatattggcg
     1261 attgattttg ccagctggtc ttccagatga ggtttagctt cttcaaactt cagattaacc
     1321 tggttggcac tggatacaac ccgggtctgg tatttatgct ggttgccggt ttctgtgctt
     1381 gtctgaattt tggagcctga tgttccctga cgcaaagctg caacattatc cgttctgacg
     1441 ctggttctgg ttcgctcggc aatctgaaca tctgtaatca ttgtgtaatt tatatcttcc
     1501 accattgcat ccgcggccat gccaatcaga ccggtagcca gtccaacacc gagagtggta
     1561 cctgccgacc cggtattgta cgctgtgatt ccacctccaa gagcagctcc gactgctgca
     1621 ccttcataac cgcgactcag ccatccctga gattcacgca gatccatttt gtctgctttg
     1681 agaacgttag cctggatcca gtagtaagct ttatccgggg aagtcatgat ctgataacct
     1741 ctggctttga ctgcatcggc aatttttccc tgtagtccac tcatgtcttt atccgacgtg
     1801 tttttgatct gcaggaaaac ggtgcgttcg ctcgcaggtt caagccagat ggttgaggtg
     1861 tactg
//
LOCUS       DAZFKM010000344         1829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFKM010000344 DAZFKM010000000
VERSION     DAZFKM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            225..734
                     /locus_tag="ACHKAY_005491"
     CDS             225..734
                     /locus_tag="ACHKAY_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5703929.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            706..>1829
                     /locus_tag="ACHKAY_005492"
     CDS             706..>1829
                     /locus_tag="ACHKAY_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5703930.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLD"
BASE COUNT          470 a          420 c          494 g          445 t
ORIGIN      
        1 ggtcctttcc ggcgatccga caggttacgg ggcggcgacc tcgcgggttt tcgctattta
       61 tgaaaatttt ccgggaacca tgtccggttt ctcttcaagt taactatatg aaaaatataa
      121 aaacaggtct tctgtgaacc ggacatgcac aaaaaataga tatgtaaacc ggacatgacc
      181 ggttttgttg tgattgtgag gtgagagttt ttgcgaggtg aggagtggct acgcagactg
      241 aagttgccag gcatttaagt ctgaccgatc gccagcttcg cagattgcag aaattgccgg
      301 gtgccccgat atcaaataag cgagggcaac tggatctgga tgcctggcgc gatttttaca
      361 tatcgtatct gaggagaagt aaaaacgatg tgcctgatgg cgatagcgaa gacgactatg
      421 aggaaaaatt gcttattgcc agatgggaac tgacagcaga acaggctgtt acacagcagt
      481 taaaaaatga ggtgtcaaaa ggaaaactta ttgacaccgg gttctgtatt tttgccctca
      541 gtaagctggc aatggcgtta tccagtacgc ttgattccat ccctttatcc atgcaacgac
      601 agtttcctga tttaacaccg cgccatcttg accatctgaa aacccttatt gcgaaggggg
      661 caaatcagtg tgcgcgggcg ggggataaat taccggattt actcgatgaa tatatcagag
      721 caacaactga ataatatgat gagcgctgtc acaactgcat tacagcccct gataagggca
      781 ttgccggtga cgccagttga atgggctgat caaaattatt atctgcctaa agaatcttca
      841 tatggtgagg gcgaatggaa aacgctgcca ttccagatcg ccatcatgaa cagcatgggg
      901 aatgatcaga tccgcactgt taatctgatt aaatctgccc gtgttggcta tacaaagatg
      961 ttgctggggg tggtcgggta ttttattgag cataaatccc gaaacagtct gctttttcag
     1021 cccacggatt ctgccgctga agattttatg aagtctcacg tggaggcgac gattcggaac
     1081 gtgccatgcc tgaaagacct ttccccatgg ctgggtcgta aacatcgtga caatactctc
     1141 acgctgaaac gcttttcatc gggcgtcggt ttctggtgcc tgggcggcgc tgccgccaaa
     1201 aactaccgtg aaaaatccgt ggacgtggtc tgctatgacg aactttcctc gttcgagccg
     1261 gatgtcgaaa aagagggctc gccaaccctg ctgggggata agcgtattga ggggtcggtg
     1321 tggccaaaat ccattcgcgg ctcgacgcct aaaatcaaag gcacctgcca gatcgaaaaa
     1381 gccgctaacg agtcggcgca ttttatgcgt ttttatgtgc cctgcccgca ctgtggggag
     1441 gagcagtatc tgaaatttgg cgatgagtcc acgccttttg ggcttaaatg ggagaaggac
     1501 agccctgaaa gtgttttcta cctctgtgaa catcatggct gcgtgatcca tcagtctgaa
     1561 ctggaccaga gcaacgggcg gtggatctgt gaaaacacgg gcatgtggac ccgtgacggt
     1621 ctgacgtttt tcagcgcccg gggtgatgaa attccgccgc cgcgctccat cacgttccat
     1681 atctggacgg cgtacagtcc gttcaccacc tgggtacaga ttgtctatga ctggctggat
     1741 gcactgaaag atcccaacgg cctgaaaacc tttgtgaaca ccacgctggg cgagacctgg
     1801 gaagaggccg tgggcgaaaa actcgatca
//
LOCUS       DAZFKM010000345         1820 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFKM010000345 DAZFKM010000000
VERSION     DAZFKM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            186..452
                     /locus_tag="ACHKAY_005493"
     CDS             186..452
                     /locus_tag="ACHKAY_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703931.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            449..1048
                     /locus_tag="ACHKAY_005494"
     CDS             449..1048
                     /locus_tag="ACHKAY_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5703932.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1041..1328
                     /locus_tag="ACHKAY_005495"
     CDS             1041..1328
                     /locus_tag="ACHKAY_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO5703933.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1321..1776
                     /locus_tag="ACHKAY_005496"
     CDS             1321..1776
                     /locus_tag="ACHKAY_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703934.1"
                     /translation="MFDFPRPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQEPVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
BASE COUNT          381 a          523 c          485 g          431 t
ORIGIN      
        1 tttccagctc cttccagagc gttttaaagc tatctggcgc tgatgtacaa aaaatacaca
       61 gccattgtaa ggctgccgga acaaattccc cctgttgcgt ctgccgaaaa tattcacaaa
      121 ataaagcgct acccgaagcc ggacggactt atccggtgct gtatggacat taacgaggta
      181 gcccgatgca agctgttttt tcttccccgt ctcccgcccc agtgacgcca ctgatgccgc
      241 tgccggacat cacgcaggag cgttttttac gtctgccgga agtgatgcac ctgtgcggcc
      301 tgtcacgctc gaccatctat gaacttatcc gtaaggggga atttccgccg caggtgagtc
      361 ttggcggtaa aaatgtggcc tggctgcact ctgaagtcac cgcatggatg gccgggcgca
      421 ttgccggacg caaacggggg tacgacgcat gatgatgccc gctctgcaaa aactcccttt
      481 ttctggcttg cctttttccg gcatttgcgg atatagtttt tccgctgccg caaaatcggc
      541 agccgggcgt gagaacccga gcaattcaca ggcgacatat gacgcgccac gcgtcttttt
      601 ttgtgtcgca atcaatgcca cagagcgcca gattatggtg tggtgtgtgg tcagtcgtgc
      661 agatggcatt ccgtgcatcc tgcccgcgtc cgctcattat gccgcagagt caatggtagc
      721 tcaggcgggg cagcctccgg gctggccggt atcctgtgaa gccggtattc tcacccccgt
      781 ctgggctatc gccatcgagc gtgagaactc cggcgatagc gttatttgct attcacagga
      841 ggctgccatc atggctacaa cccttacccc ctcacaccct gaatttgtct ttgtgtttgc
      901 ggctgtccgt cgcgcagacc gtcatccccg tatctgtatg cttcgcaccg tcgccggtga
      961 tgaacgcagt gcccgccgtt cccttgtccg tgactatgtg ctctcccttg ctgcccgtct
     1021 gccggtggtg gaggtgtccc gtgcgtaata aaaaagcccc tcagaccgtc tcagcacgtc
     1081 atgacgcccg tgaacacctc agcattgagg cttaccataa gctcaaccgc gccagcgccg
     1141 tatcccagtt tgttgggggt gatttgattc accgtgaact ctccggcctg catcagctct
     1201 acattccgca tattttcagc tacctgaatg aagatattga ttttgtgctg aatgagctga
     1261 aagccaaagg cctgtgccgc gattttctcg cccagcagaa agaccgggga gacaggacgc
     1321 atgtttgatt ttccccggcc cggtgagatt taccgttctg ccggttttcc cgatgtggcc
     1381 gtggtcggca ttctggaaga cggtattccg tgggaaatgc cgtaccgctg cccggaaatt
     1441 gtctggaacc cgtaccgccg taaattcagt atccttgtgc gtatcctcgc tgacgggcgc
     1501 accacagaca tcccgctggg gcgttttctg cgggaattta cctgtgaccg tcctgacctg
     1561 tttaaacgca gccccgtaaa ccggcatgcg gtactgaaag aaatggccgg agacccggaa
     1621 ttacagaaat ggcgggagaa atatctggat atttacccgc aggagcctgt tccggtcagc
     1681 cgggcggcac cggtggcgcg ggaatggcgg gaaattcccc gcacggagcc tgacccggaa
     1741 atcaccccgg ataacagtta ccgcaattat ctgtaattaa aaaacgacac ccgaaaaaaa
     1801 aattaaatgt gcgtattcgc
//
LOCUS       DAZFKM010000346         1818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFKM010000346 DAZFKM010000000
VERSION     DAZFKM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..397
                     /gene="escR"
                     /locus_tag="ACHKAY_005497"
     CDS             <1..397
                     /gene="escR"
                     /locus_tag="ACHKAY_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO5703935.1"
                     /translation="INYTDSDFFQKVDEKILSPYRGFLEKNTEKENVEFFERAAQKKL
                     GNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSP
                     VTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            397..666
                     /gene="escS"
                     /locus_tag="ACHKAY_005498"
     CDS             397..666
                     /gene="escS"
                     /locus_tag="ACHKAY_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO5703936.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            666..1442
                     /gene="escT"
                     /locus_tag="ACHKAY_005499"
     CDS             666..1442
                     /gene="escT"
                     /locus_tag="ACHKAY_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO5703937.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILLLVIHVFPDFITANIHSDIIDRSLPSMINE"
     gene            1435..>1818
                     /gene="escU"
                     /locus_tag="ACHKAY_005500"
     CDS             1435..>1818
                     /gene="escU"
                     /locus_tag="ACHKAY_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO5703938.1"
                     /translation="MSEKTEKPTPKKLRDLKKKGDVTKSEEVMAAVQSLILFSFFSLY
                     GASFFVEVVELVNTTIDSLNRPFLYAIREILGAVLNIFLLYILPISLIVFVGTVTTGV
                     SQTGFIFAVEKIKPSAQKISVKNNLK"
BASE COUNT          542 a          289 c          330 g          657 t
ORIGIN      
        1 aatcaattac accgactctg atttttttca aaaagttgat gaaaaaatat tatcaccata
       61 ccgcggtttt ttagaaaaaa atacagagaa agaaaatgtt gagttttttg aacgtgcagc
      121 tcaaaaaaaa ttgggtaatg aaactatctt aaaaaaagac tctctattta tactgttacc
      181 agctttcacg atggggcagc ttgaagctgc attcaagata ggttttttgc tctatttacc
      241 ttttattgcg atagatttga tcatttccaa tatcttattg gccttgggta tgatgatggt
      301 atcgccagta acaatttcga ttccttttaa gatcttgtta ttcattcttg ttggaggctg
      361 gcaaaaatta ttcgaatttc tgttggtggt gaattgatgg aaactggata ttttgttcaa
      421 ttatgtgtgc aaacgctttg gataatattt atcctctcac tgcctacagt catagcggcc
      481 tctgttgtcg gcattattat tagcctggtt caggctataa cgcagttaca ggatcaaaca
      541 ttgccttttt tgctaaaaat aatagcagtg tttgctacgc ttgccctgac ttatcactgg
      601 atgggaacaa caatcatcaa cttctcgtcg ataatatttg aaatgattcc gaaggtgaat
      661 ggctaatgaa tgaaataatg acggtcatag tatcatcatt tttttgtata ctcaggccgc
      721 tggggatgtt tattattcta ccaatatttt caacaggtgt tttgctcagt aactttatac
      781 gcaactcaat tatgattgcg tttacacttc caattatagt agagaactat acattttcgg
      841 aaaaattacc atccggaatt tttcagttga caggcatagt gctaaaagaa ataagtatag
      901 gctttttcat tgggttatca tttactattc ttttttgggc aatagatgcg gctgggcaga
      961 ttattgatac tttaagaggt tcaacaatat cttcaatttt taacccgtct ataagtgatt
     1021 catcttctat cactggtgtt attttatacc aatttatcac tgtgatcttt gttattcatg
     1081 gtgggataca aagcgttctg gataagttgt atttatccta cgagatatta ccattacaag
     1141 ccgatattgc attcaatcgt gctttagtag attttttatt ttcgctatgg gattcattta
     1201 ttaaactgat gttatcattt tcaattccca tgattatcgg tatcttctta tgtgatatgg
     1261 ggtttgggtt tctcaataaa acagcgcctc agctaaacgt attcacatta tcactgccgg
     1321 taaaaagttt gatagcaatc tttatattgt tattagttat tcatgttttc cctgatttta
     1381 ttacggcaaa tattcattct gacataattg acagatcgtt accgagcatg attaatgagt
     1441 gaaaaaacag aaaagcccac acccaaaaaa ctaagggatc taaaaaagaa gggcgatgta
     1501 acaaaaagtg aagaggtaat ggctgcagtg cagtcattaa tcttattttc atttttttct
     1561 ttatatggtg cgagtttttt cgttgaggtt gttgagttag ttaatacgac tatagactcg
     1621 ctaaatagac cgtttttgta tgccattcgt gaaatattag gtgcggtatt aaacatattt
     1681 ttattgtata ttttgccaat ttctttgatt gtctttgttg gaactgttac aactggtgta
     1741 tcacaaacag gattcatctt tgcggttgaa aaaataaaac catcggctca gaagattagt
     1801 gtaaaaaata acctgaaa
//
LOCUS       DAZFKM010000347         1800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFKM010000347 DAZFKM010000000
VERSION     DAZFKM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHKAY_005501"
     CDS             <1..198
                     /locus_tag="ACHKAY_005501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703939.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>1800
                     /locus_tag="ACHKAY_005502"
     CDS             234..>1800
                     /locus_tag="ACHKAY_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703940.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAPRDLASFRICSDFFDLLVGIKNNKLKIDFESYDKPFHLADIRELLLLMKIL
                     NAPSGDLKLTIKNNEGNETSFLASDNSFSANPITNEIIKAEQIALALIQVASYFRVDK
                     NCKVSINELIRKQKDIERMHTLISKVPGGYPALRLEIPEHDDNIDSSKELRIFIAIPL
                     IFSSFSICLTCVARGSFTYQNNAMTFNDYEINIEKLIRATNLNELKKQCQAVSDKVNK
                     KFEKDSMDFYYYNS"
BASE COUNT          608 a          294 c          342 g          556 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat cgaaataaga tctgttgaag cattctcaac aggctctata atattcgaag
     1021 atgaaaaaga agatgtctct tttaagtgtg aatttttcgt ttctcctatt gcacttaatg
     1081 ctccaaggga tctggcaagc tttagaattt gctcggactt tttcgattta ctggttggta
     1141 ttaaaaataa caaattgaaa atagattttg aatcatatga taagcctttt catttagctg
     1201 acattagaga attactgtta ttaatgaaga tattaaatgc cccaagcgga gatctgaagt
     1261 taacaataaa aaacaatgag ggtaacgaga catccttttt agcctcggat aattcatttt
     1321 ctgcgaatcc aattactaac gaaataataa aggcagaaca gatagccttg gctttgatac
     1381 aagttgcttc atactttaga gtagataaaa attgtaaagt aagtataaat gaattaattc
     1441 gcaaacaaaa agatattgaa aggatgcata ccttaattag taaggttccg ggcggctatc
     1501 cagctcttcg cttagagatt ccagaacacg atgataacat tgactcttca aaggaattaa
     1561 gaatatttat cgccatccct ttaattttct catctttttc aatttgcctc acttgcgttg
     1621 ccaggggctc ctttacatat caaaataacg ctatgacatt taatgattat gaaatcaata
     1681 ttgaaaaact aattcgcgca actaatctta acgaacttaa aaagcaatgc caagcagttt
     1741 ctgataaagt aaataaaaaa ttcgagaagg atagtatgga tttttactat tacaactcat
//
LOCUS       DAZFKM010000348         1782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFKM010000348 DAZFKM010000000
VERSION     DAZFKM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..610)
                     /gene="nleH1-2"
                     /locus_tag="ACHKAY_005503"
     CDS             complement(<1..610)
                     /gene="nleH1-2"
                     /locus_tag="ACHKAY_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO5703941.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDN"
     gene            complement(717..788)
                     /locus_tag="ACHKAY_005504"
                     /pseudo
     CDS             complement(717..788)
                     /locus_tag="ACHKAY_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            782..1776
                     /locus_tag="ACHKAY_005505"
                     /pseudo
     CDS             782..1776
                     /locus_tag="ACHKAY_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          532 a          431 c          381 g          438 t
ORIGIN      
        1 cattatcatt atatattttc tctgcactcc cggaaccata atactgattg aaacaacgaa
       61 cttcgcttgt cacctcttca tggctttgag atatagtaaa catcttcaac acttttgttg
      121 tatcttccat atcttcatac acgacagcat taccaccttt accaataaca ttacctggca
      181 cgggtgattt attgttgcta accctcggca actctgattg agtataatcc gctggaggta
      241 actccggctg cgcataatcc actggaggta aatcaggtct gttcgaatga acagagcctc
      301 tttctatagt cgtgctcact ggcgatgtat tcagcatagc ctcaattttt ctgctaatct
      361 ctcccttagg ccatcccaac ctgtgcaaca gattagtaaa acaaccacta tgactttctc
      421 ttgtaacgca aaacttatta tcagtgacca caacacgata tgttctgttg ccaaccgtta
      481 cttgcgtccc gctatcagag tgaacagcag catcccttac agaggataaa acacgattat
      541 caggcgaagt caggtttctg gttaaagaat tccatgaaca tcccaaattt atagaagagg
      601 gcgataacat acatttcaac cttcaaaata aacctatcta attattccta acagacatcc
      661 cccatacatg acaacaaaaa ccggagccgg actccggttt ttgtgaagct atcgggttac
      721 ttcatttcgc caatattttc ccacttcccg tcagcacgca ggatttgcag cggtcttact
      781 acacactgac ccgaggacga aaaaagagtt cgggctggga agagacagaa gagtagcaat
      841 ttcagaagca ttcacaccaa cattgagctt ttcagcgaaa gtgggcacga atcattgctg
      901 gacagggtta aaggcactga ctcaatcact cttcatacgt ggcttgatcg atatgaaaca
      961 atcctcagct agagggggag caagccgaaa acattactcg actacgccag caaaatcagg
     1021 gcaattcgaa gaaaattgcc ggacaaaccg ctcactgaca tatcaacgaa agaggtggca
     1081 gcaatgctaa acacctacgt cgcagaaggt aaagcggttt ccgcaagagt aatcaggtca
     1141 acccttgttg acgtttttcg aggggcaata gccgaggggc atgtggcaac gaatccagta
     1201 acaacaaccc gtgcagcaaa gtcagaagta aggcgctcaa ggctgacagc taatgagtat
     1261 gtcgctattt accatgctgc tgagcacctc cccatctggc tgaggctgtc aatggattta
     1321 gctgtcgtta cagggcagag agtgggcgat ttgtgcagaa tgaagtggtc agacataaac
     1381 gatggtcatc ttcacattgg acagagtaaa acaggagcca aaattgccat tccgctggct
     1441 ctaaccattg acgcactcga catctcactg gttgatacac tacagaaatg cagggaggcc
     1501 agcagcagtg aaacaataat cgcatcaacc tatcacgaac cactttctcc agccacagta
     1561 tcacggtatt taacaaaggc gcgaaatgca tccgggatct cgtttgatgg agacccaccg
     1621 acatttcatg aactacgtag tctgtccgcg aggctatatc ggaaccagat tggctacaag
     1681 tttgcacaac gtcttcttgg acataaatct gattcaatgg cggcgcatta tagggacagt
     1741 cgcgggcggg agtgggacaa aattgaaatc ggataatgat tt
//
LOCUS       DAZFKM010000349         1768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFKM010000349 DAZFKM010000000
VERSION     DAZFKM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1768
                     /locus_tag="ACHKAY_005506"
     CDS             <1..>1768
                     /locus_tag="ACHKAY_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5703942.1"
                     /translation="ATKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMAAGVGK
                     ATTELIAAKSGTVALAAAQADAAVQVQRKAAADALAAERAWQLAQAELALARNSSAEA
                     LATQNAVAKRRAMIVANAALVQSNQAVAASRKALENATSVTGMLKSGAASLLSLVGGI
                     PGLLMLGAGAWYTVYQNQEQARESARQYASTIDEIRAKVPGMTLPELADNEGKTREAL
                     EEQNRLIAEQESKVRGLKTQIADYQRWLQENPQGGSGVEIITHGLAEATGKLAVEQSR
                     LTQMQEKAKSVQEALASLEYNRVARIREEAAEQNRAYQALLQMNVQHTEFNRLLGLGN
                     ELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDL
                     GFVGDPYQEARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLA
                     LVGQNTELAKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNEN
                     AAARASNDAELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQA
                     LAQNAQYLSERLKEQEAFYAESD"
BASE COUNT          457 a          415 c          594 g          302 t
ORIGIN      
        1 gcgacgaaaa cgctctccgg cgtgctgaac ggtgttgccg gtaatattga taatgtggca
       61 acagccgcgg gggcgctggt tgccgtcggg gtggcccggt actttggcaa tatggccgca
      121 ggcgtgggaa aggccaccac agagctgatc gcggcgaagt ccgggacggt tgcccttgca
      181 gccgcacagg cagatgcggc tgttcaggtg cagcgtaagg cggcagctga cgcgctggcg
      241 gcagaacgtg cctggcaact ggcacaggct gaacttgcac tggccagaaa tagcagtgcg
      301 gaggcgctgg ccacacagaa tgccgtcgca aaacgccggg ccatgattgt ggccaatgcc
      361 gcactggtac agtcgaatca ggcggtggcg gcctcgagga aggcgctgga gaatgccacg
      421 tcggttacgg gaatgctgaa aagtggcgcg gcatccctgc tttcgcttgt cggcgggata
      481 cccggtttac tgatgctggg agccggtgcc tggtacacgg tgtaccagaa tcaggaacag
      541 gccagggagt ccgcccgtca gtatgcatcg acgattgatg aaatccgggc aaaggtaccg
      601 ggaatgaccc ttccggaact ggctgataat gagggtaaaa ctcgcgaggc actggaagag
      661 cagaatcgtc tgattgctga gcaggaaagt aaagtccggg gactgaaaac acagattgct
      721 gactatcagc gctggctgca ggaaaatcca cagggtggtt caggtgtgga gattataacc
      781 cacggccttg cggaggcaac tggcaaactg gcggttgaac agtcacgact gacacagatg
      841 caggaaaagg cgaagtctgt acaggaagct ctggcgtcac tggaatacaa ccgtgtggcg
      901 aggatacgcg aggaggcagc agagcagaac agagcatacc aggcattgct gcagatgaat
      961 gttcagcata cggaatttaa ccgtctgctg gggctgggga atgaactgct tcagcagcgg
     1021 cagggactgg tgaatgtgcc gttacggctg ccacaggcca ccctggatga taaacagcag
     1081 agtgccctga caaaaacaga gcgtgagctg gccctgtcca gactgaaagg ggaagaaaaa
     1141 gagcgtgtcc ggctggggta tgcggcggat gacctcggtt ttgtgggtga tccgtatcag
     1201 gaggcgagac aacgttatat cagtaatgcc ctggaagcct ggcgcaataa cgaggcgaat
     1261 aaacccaaat cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg
     1321 ctgctgaagc agcagaaaga gcaactggca ctggtggggc agaatacaga gctggcgaag
     1381 ctgaaatacc agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggag
     1441 ctgctgcgta atgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
     1501 gaagagacac tgaagaatga gaatgcggct gcgcgtgcgt cgaatgatgc tgaactgctg
     1561 gggtacgggc agggggaacg agccagagaa cgcatgcggg agttgcagca gatccgcgac
     1621 agcttccgcc agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag
     1681 gatttttaca gacaggctct ggcgcagaat gcacagtatc tgagtgaacg tctgaaagag
     1741 caggaagcct tttatgccga atcggatg
//
LOCUS       DAZFKM010000350         1762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFKM010000350 DAZFKM010000000
VERSION     DAZFKM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..1358)
                     /locus_tag="ACHKAY_005507"
     CDS             complement(171..1358)
                     /locus_tag="ACHKAY_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFO5703943.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRRLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQEWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1358..>1762)
                     /locus_tag="ACHKAY_005508"
     CDS             complement(1358..>1762)
                     /locus_tag="ACHKAY_005508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703944.1"
                     /translation="YKNRQYHCLSGSYMSRSAKPRKRKPAPQRSKLPRYVVKLHDDDF
                     FDEEDAEALRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTER
                     EFATILAGISAGGMYCPECGTVHWPDGVPPPF"
BASE COUNT          415 a          523 c          412 g          412 t
ORIGIN      
        1 tcccaaaaag attaaaaaaa cacccaaaaa cggatgttta ttcaacactg cctgcgtgcc
       61 atgagtgcac cgccgtcaat taaatggatc ttatcgattg atactgcata tcactgatgg
      121 ttttttaccc cattcccctg aaaatggacg caacttctcc gatgcggctc tcagccacac
      181 acccgctgtt gcgccagtgg ctcatgcatc aggaccagct ctgtcagacg gtagccccgc
      241 ttcagccccg taaaacgcat ctgacctccg cacaggatgc acttcagcgg gtcaaccttc
      301 agtaaccgct gatacatccc tctccacctg atttgcatcg ccgtctttct caccgtctcc
      361 gttatgacgt acacaacatc ttccagtaac cggcgcttca ccggactcag gaaaccgtag
      421 tacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc cataaactca
      481 tcaccactca tcaccaggta ttcttcccgt tttgtccggt gactgttgta acgcagacca
      541 atttcatcct gaccagcata gtgctccaga cggctcatcg gcaccggcgg ttttttcagg
      601 taagagccga agtacaccgc cacatgtgtg gcgttatcca tcacccggga tacgttgaca
      661 ttccagcccc gccggtaatg actgtccagg aagcgattcc agtcccgttt actgcttcct
      721 tctgctgcca gcgcatccgg tatcaccagg tccggatatt tccgtgacag taaccgcgtt
      781 atccggtaac gccacatact catcacctta cgggcgtaaa aatgaaggtt tttccaggtg
      841 tggtctgatg tcacgccgcc ggtcgttgtc gacaggtgaa tgtgcggatg ccactcctgg
      901 tcacgtcccc atgtgtggat caccgtgaat atccccggca ccacatctgc ctggcggcag
      961 atttcctgta tcacatccgc agcaatgcgg ctcatctctg ccagtaacct ccggttgtgg
     1021 aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca cggacagtcg
     1081 ggaaccagac tcagcagata ctgtatccac tgtgcgccag ccttcactcc gcagtgcggg
     1141 caggagcgac ttttacaccg gaagcagacc ttttttatgt ggctgcagtc cggagatgaa
     1201 cagcaccact gtgtgtaccc catcagcgtg gtcccgcagg ccatgatttt tgtcacagac
     1261 tcaatgacta ccggacgcac tgaaccttcc ggttgtttct ccagccagtt aagccagcgg
     1321 tttccctgct gaaaaatgtc ggcaaaacgg ggaagcatca gaagggcggg gggactccgt
     1381 ccggccagtg aaccgtgcca cactccgggc agtacatgcc gccggcgctg ataccggcaa
     1441 gaatggtcgc aaactcccgc tccgtgcagc gggctatttc aggataccct tcgtcatcaa
     1501 cacgtacaaa ccagaagacc agctttttgt ttccggcatc cacaaagaag ggaatattca
     1561 ggtctgcgca gcactcaacg gcatcgtcaa aattatcaaa gcgcagagct tctgcgtctt
     1621 cttcgtcaaa gaagtcatcg tcgtgaagct tcacgacata gcggggaagt ttgcttcttt
     1681 gaggggcagg ttttcgttta cggggtttag ctgaacggga catataacta cctgaaagac
     1741 aatgatattg tctgttttta ta
//
LOCUS       DAZFKM010000351         1752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFKM010000351 DAZFKM010000000
VERSION     DAZFKM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..397)
                     /locus_tag="ACHKAY_005509"
     CDS             complement(77..397)
                     /locus_tag="ACHKAY_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5703945.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            505..678
                     /locus_tag="ACHKAY_005510"
     CDS             505..678
                     /locus_tag="ACHKAY_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO5703946.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            749..1672
                     /locus_tag="ACHKAY_005511"
     CDS             749..1672
                     /locus_tag="ACHKAY_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO5703947.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGELIGKQEKKTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSCRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWLRLKNKELPPSLHHY"
BASE COUNT          491 a          381 c          386 g          494 t
ORIGIN      
        1 atgcgcggca tggtggctaa ttttggcggt ttcctttcca tcaataaact ttcgcagtaa
       61 tggattatgc ccaccgtcag gtgggttttt tatttagtag ttctctcaac ttttcgttct
      121 gctctctgaa cttttcctgt atttcttttt gcatggcgat aacctgggct tgaagttgaa
      181 ctagcgcatc aacatttagc ggaaccgaga cgctgttaat tttatctgct atttccccga
      241 gtgtattttc tgcattcaag gcatcttcca gtatctgaac aatctctgag ttcatagagc
      301 gcccgtttcg tttggctcgt tcagctatag catctcgcat tccgtcagga aaacggaggt
      361 tgaacttgtc gtagtctttt acttgttttt cggccattgc aaatctctca aaaaaaatca
      421 tggtgccata ttgccatacg atttcaatag tggcattatg gccttcatgg tgtcactttg
      481 gcaccaaata aaggagatta gataatgcaa gatacacttt tcactgagcg caaaaatatc
      541 aaactcaacc ttcgccttcc atcacggctg aatgaagacc ttcgccgcct ggcggaaatg
      601 gactgtatat ctctgaactc tgcaattgtt cgtttgctgg caaaaggtgt tagggaagag
      661 gtggcgaatg gtcgctaaaa acagcgaagc ctcaatggct gcaaccattg aggcttctaa
      721 attaccagtt aaccacgaga aaactgatat gactagttta gcaattgcag atcgcacaat
      781 caatgttcca ttccacggaa caaatctctt tttggttgga attaacaatg agccttatgt
      841 tcctatgaag cctgttgttg aaggtatggg gatggtttgg gctgctcaat ttgttaagtt
      901 aaaacagagg tttgtcaaag gtatttcgga aatcgaaata ccttctgctg gcggtaaaca
      961 gttaatgaca tgtcttgcct ttcgtaaatt tgcggcttgg ctttcaagca ttcaaccaaa
     1021 caaagtccgc cctgaaatcc gcgacaaggt aatccagtat caggaagagt gtgacgatgt
     1081 gctctacgag tactggacta aaggccatgt gattaacccg cgcaaagcta aaaaggcatt
     1141 gccggggaaa atcaccactg aacagcagga agccattaaa caactcgtca tgagtcgcgg
     1201 tcagtctctg ccaaaagaaa aacaggcgaa ggcgatgatc accatgtggt cgtcactgaa
     1261 atctcatttt gggtgttcat acaaagaaat cagcgaggag cagtttaccg aagctctgtc
     1321 acttgctgct cgcgttccgc ttgaaggaga gttaatcggc aaacaagaga agaaaaccaa
     1381 cgagctttct gcaaaagaag caaacagcct tgtatggtta tgggattatg ctaaccgctc
     1441 acaggcatta ttccgcgaac tgtatccggc gctaaagcaa attcaatcga actattcctg
     1501 cagatgctac gactacggtc atgagttctc gtatgttatt ggaatggcga gagacgtttt
     1561 aatcaatcac acgcgagata ttgatattaa tgagccagac ggaccaacga atctttctgc
     1621 atggctaaga cttaagaaca aagaattacc tccttcactg catcactact aacagattgc
     1681 caacgaaatg acccagcttc ggctgggttt tttatcagga gttctcatgc tctatagcaa
     1741 tatattggcg ca
//
LOCUS       DAZFKM010000352         1745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFKM010000352 DAZFKM010000000
VERSION     DAZFKM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1563
                     /locus_tag="ACHKAY_005512"
     CDS             <1..1563
                     /locus_tag="ACHKAY_005512"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO5703948.1"
                     /translation="SKDIPIVNEYSSDLSDEHLLSSHIIYSNIQKVTSSRGNPLISRV
                     PPDFFDLIIIDEAHHAPAQSWRDTLHYFSNAKILHVTGTPYRGDNQEIPGVKIHETPL
                     SEVMRDKYVKWLRKETVNAHDLFFYTPEQPGVKLSKEQVLMFKDKEWIEKSIALSKEC
                     SLDVINHSIKKLNELKELSPNVPHKILAIGCSISHAEDLLSWYKAKGMTSVIVHSEMD
                     QNDLSDALMKIEHHQCDVVISVNMLMEGYDHKYLTVLAIFRPYRSLNAFAQVVGRILR
                     AIPESEITHFEIDNNGIVIFHEEIGLNVMWETFQKEVERARRASVREYTFTDRDYHER
                     SNTLAGIESDSAYVSDTDSYLDDVDFNAMFEQKRNEINNSVEQQIEKIKSSGVDLPED
                     ALEALRQTLAEKAVQKAAEIIDPELIEKRPHQARKLMRNILKTRTQDGVSNLLIDNGI
                     GEKSSELYSIFNRHVPYLKTTDPNDGILVRYVNLKLAKAYGPVDKRDNKTLLQSINDL
                     DKILAEVERMIK"
     gene            1560..>1745
                     /locus_tag="ACHKAY_005513"
     CDS             1560..>1745
                     /locus_tag="ACHKAY_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703949.1"
                     /translation="MTNYSQIMEEINKIISFCMVKGVQPHELISAIFEDEYKHIETYK
                     KGEHIHLILSYSDTHEDG"
BASE COUNT          640 a          281 c          320 g          504 t
ORIGIN      
        1 agcaaagata taccaatagt taatgaatac tcatctgatt tatctgatga acatctttta
       61 agcagccaca taatttattc aaacatacaa aaagtaacat cttctcgagg caacccacta
      121 ataagtcgtg taccccctga ctttttcgat ctcattatta ttgatgaggc tcatcatgcc
      181 cccgcacaaa gttggcggga tactcttcat tatttttcca atgctaagat attgcatgtt
      241 actggcactc catatagagg cgacaatcaa gaaataccag gagtaaaaat acatgaaact
      301 cctttatctg aagttatgcg ggataaatat gtcaaatggt taagaaaaga aaccgtcaat
      361 gcacatgatt tattctttta cacgccagaa cagccaggag taaagttatc taaagaacaa
      421 gttttgatgt ttaaagataa agaatggata gagaaaagta tcgctctttc caaagagtgc
      481 tctttagatg ttattaatca tagtataaaa aaacttaatg aactaaaaga gctgtcacca
      541 aatgtcccac ataaaatttt ggctataggc tgtagtatat ctcatgctga agatttactt
      601 tcatggtata aagccaaagg aatgacatca gtaattgttc atagtgaaat ggatcaaaac
      661 gacctttctg atgctctaat gaaaatagaa catcatcaat gtgacgtcgt tatatctgtc
      721 aacatgctca tggaagggta tgatcataaa tatctaacag tacttgctat atttagacca
      781 taccgtagcc taaacgcttt tgcacaagtt gttggacgca ttttacgagc gattcctgaa
      841 tcagaaatta cccactttga aatagataac aatggaatag tcatattcca tgaggaaata
      901 ggtttaaatg tcatgtggga aacatttcag aaagaagtgg agagagcacg cagagcaagt
      961 gtaagagaat atacatttac agacagggat tatcatgaaa gatctaatac attagctggg
     1021 atagaaagtg atagtgctta cgtaagtgat acagactctt acctagatga tgttgacttt
     1081 aatgctatgt ttgaacagaa gcgtaatgag atcaacaatt ctgttgagca acaaatagaa
     1141 aaaataaaat cgtctggagt agatcttcct gaagatgctc ttgaagcatt acgccaaaca
     1201 ttagcagaaa aagctgtgca aaaagccgca gagataattg atcctgagct aattgaaaaa
     1261 aggcctcatc aagcgagaaa gttaatgaga aacatcttga aaacccgcac tcaggatggt
     1321 gtttctaatt tattgattga taatggaatt ggtgaaaaaa gctccgaact gtatagtatt
     1381 tttaaccgac atgtacctta tttaaagaca actgatccaa atgatggtat attggttaga
     1441 tatgttaacc taaaattagc caaagcatat ggtcctgttg ataagcggga taataaaacc
     1501 cttcttcaat caataaatga tttggacaag attctcgcag aagtagagag gatgattaaa
     1561 tgacaaacta ttctcaaatt atggaagaaa ttaataaaat aatttctttc tgtatggtta
     1621 aaggtgttca acctcatgaa ttaattagtg caatattcga agatgaatat aagcacattg
     1681 aaacttataa aaaaggtgag catatacatc ttatattgtc ttattcagac actcatgaag
     1741 atggt
//
LOCUS       DAZFKM010000353         1712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFKM010000353 DAZFKM010000000
VERSION     DAZFKM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..436
                     /gene="yqeJ"
                     /locus_tag="ACHKAY_005514"
     CDS             <1..436
                     /gene="yqeJ"
                     /locus_tag="ACHKAY_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO5703950.1"
                     /translation="GFILFTVYSYTAEKMIYNETCTANWVIFNDQGRANLTIDFMYNK
                     KNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDR
                     LAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(469..549)
                     /locus_tag="ACHKAY_005515"
     CDS             complement(469..549)
                     /locus_tag="ACHKAY_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703951.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(585..1010)
                     /gene="yqeK"
                     /locus_tag="ACHKAY_005516"
     CDS             complement(585..1010)
                     /gene="yqeK"
                     /locus_tag="ACHKAY_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO5703952.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            1204..1694
                     /locus_tag="ACHKAY_005517"
                     /pseudo
     CDS             1204..1694
                     /locus_tag="ACHKAY_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          636 a          294 c          266 g          516 t
ORIGIN      
        1 tgggtttatt ctcttcactg tctattctta cactgcagaa aaaatgatat ataacgaaac
       61 atgcactgca aattgggtaa tttttaatga ccagggacga gcaaatctta ccatcgactt
      121 tatgtataac aaaaaaaata aaacaggaac tgttgcgttg agtggaacct ggcaacaagg
      181 taacagagaa agtaaatcaa tacggcgaaa tattgaatac acatggattg aaaactatga
      241 cacagcccat ttaacatcaa agaaagttaa taagtttgag attatggatc aagttgatga
      301 tgatagactt gcacaactta ttcctgattt ttatgtcttt ccagaaaaaa gtgtaagcta
      361 taatatacta aagcaaggta agcatgcttt tattttgagc attggtaaca gagcaataat
      421 gcattgtgca aggtaaacta aaatagataa caaaaagatg gaacctcgtt aatcataaaa
      481 caataaaatt aaatattctc gcagtatatg gcagtctaaa gcatcaaagt tttgatcaac
      541 atctttcatt ttagacatct ccttgcaatg taaaatatca tgtatcaaca acatctggtg
      601 caaatccatg agtcgaactc gacattcatc ttagctcgat tattattcct tcgtacagtc
      661 gatgtaaaca atacagaaag aggattatta agaaccagtt tactcagtga atcaatagag
      721 gaaaggacta acgtttcttt aaaagaattg atttcatcat ctgttaaact aaactcatca
      781 ttgacagatc gtgagatata actgctttta actttactct ttacgttgac tttattgaca
      841 gaattaacat tcacaaatct tgaatttaat gcccatgatg ttgtttgcgt gagaaccttc
      901 tcagcattaa ggaatttttt taacggaatc cttggtttct ttttcgagtc cgaatttaca
      961 atatcatgca tatagaccat ttcatgaatc tgcgaaaact caatgtccat tatatacctc
     1021 acatttttac cgtgactcga tgttactgtt caataatcac cttccatcaa tactaaaatt
     1081 aataccccta atgtgccgat aacaaatata gtcattctac gtaatgtctc cataaggtga
     1141 tatttgacat tatcagaagc tgcgaattcg gattttgctc taatcaaatg agagaaatgt
     1201 aaaatgaagc cacgaaatat taataatagc ctaccactgc aaccattagt tcctgatcag
     1261 gagaacaaaa ataagaaaaa tgaagagaaa tccgttaatc cagttaaaat cacaatgggg
     1321 tctggtttaa attatattga acaagaatct cttggaggaa aatatctaac acatgatttg
     1381 tcaataaaga tagcggatat ttctgaagag ataattcagc aagcaatatt atctgctatg
     1441 agcatatata aattttcgat aacagatgat ttaatgagta tggctgtaaa tgaactcata
     1501 aaactgacca aaatagagaa taatgtagac ctgaataaat tcactactat atgcacagac
     1561 gttctatccc cccgcgtcac cagacataat aaagaaaaaa aacaaacgac attctaccct
     1621 tctcaaaaat ccccttttta attttcattg aaaaaatggc aattgatact atgaatccag
     1681 gtcatacaca ttaaggcatt tatgtaatgt cg
//
LOCUS       DAZFKM010000354         1695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFKM010000354 DAZFKM010000000
VERSION     DAZFKM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHKAY_005518"
     CDS             <1..882
                     /locus_tag="ACHKAY_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5703953.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
     gene            1173..1526
                     /locus_tag="ACHKAY_005519"
                     /pseudo
     CDS             1173..1526
                     /locus_tag="ACHKAY_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          462 a          400 c          406 g          427 t
ORIGIN      
        1 ttccccttta tcggcaaccg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagcaaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgctgtaa tgcgttacgc cgtacagtct ggcatcatca gatataaccc ggctctggat
      181 atggctggtg cattgaccac ggtaaaacgc cagcatcgcc ccgctctgaa tctttcacgc
      241 ctgcctgaac ttctgtcgcg tattgacggt tataaaggcc agcctgtcac ccggcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatatgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggctgtag ccatactgac agaactacag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccgcg taaaccaatc agtgaaaata ctgtaaataa agccctgagg
      601 gtgatggggt atgacacaac ccaggatgtc tgtggccatg gattccgggc gatggcgtgc
      661 agtgcattga ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca gatgagccat
      721 caggagcgta atggtgtacg tgctgcgtat atccataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt cttgatgcga accgggagaa ggggatcagt
      841 ccgtttgaat acgcaaatgt taacaatccg ctgagaaggt gacagaaggc tattcacaca
      901 catgctcttc ccatggggaa gagcaacctg ctccaataaa tttctttttt attccggctg
      961 atagcaccgc aatcacccgt tacctctaaa tagccactta gcattcggcg tataagttga
     1021 cagagaagga gtatctcagc aggagcttaa agtgtggttt taggcagaat ttgattgatc
     1081 ctccatttgg ggcatgtgac taatcgatat agcttcctga acggaaaaag tgttttccct
     1141 tccaatataa actattcgac aataatataa atcaattaca gaataaaact ctgtataaac
     1201 atgcaaaaat ggtaaaggcg ttcgactata tcctgaatca ctggaatgcg ctgaacgagt
     1261 tctgtcgtga cggctgggtg gaaatagaca acaacatcgg tgaaaacgcg ttacgatcgg
     1321 tggcggttgg aagaaaaaat tatctcttct tcggctcaga caagggggga gaaagcgcgg
     1381 cgatcatcta cagtctgctg gtcacctgca aactgaacga agtggagccg gaggactggt
     1441 tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgtgtgcat gaactgctgc
     1501 cctggaactg ctcaactgta aaataatcct tacgctacgt acttctcggg gcgcttacga
     1561 atttttcacg tgccacagaa ttttgaacgc tttctcttac aatatttcaa tgtttctatc
     1621 agtattcgcc ggaagaagtc atcgaccaaa tcatcccagt cgtctcgcac cactgaccat
     1681 tcatggttga catgt
//
LOCUS       DAZFKM010000355         1689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFKM010000355 DAZFKM010000000
VERSION     DAZFKM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..730
                     /gene="yiiX"
                     /locus_tag="ACHKAY_005520"
     CDS             122..730
                     /gene="yiiX"
                     /locus_tag="ACHKAY_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO5703954.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            875..1550
                     /locus_tag="ACHKAY_005521"
                     /pseudo
     CDS             875..1550
                     /locus_tag="ACHKAY_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          443 a          422 c          392 g          432 t
ORIGIN      
        1 tattgcaatt tattgaattt gcacgaactt gtaggccgga taaggcgttc acgccgcatc
       61 cggcataaac aacgagcacg ttgtctgcga cccaccgctt tttatacatg gacgtttaac
      121 tatgaaaaac aggctgctga tcctcagcct gctggtttct gtacctgcct ttgcctggca
      181 gccgcaaact ggcgacatca tctttcaaat ctctcgctca tcgcaaagta aagcgatcca
      241 actggcaacc cattccgatt acagccacac cggtatgctg gtgatgcgta acaaaaagcc
      301 ctacattttt gaagcggtcg gcccggtgaa atacaccccg ctcaagcagt ggattgccca
      361 tggtgaaaag ggcaaatacg tcgttcgccg cgttgaaggc ggactgagtg ttgaacaaca
      421 gcaaaaactg gcgcaaacgg caaaacgtta tctcggtaaa ccgtatgatt tcagcttctc
      481 atggagcgac gatcgccagt actgttcgga agtggtgtgg aaggtttacc agaacgcgct
      541 gggaatgcgc gtgggcgaac agcagaagtt gaaagagttt gatctcagca acccgctggt
      601 ccaggcgaag ctcaaagaac gctacggtaa aaatattccg cttgaggaaa cggtagtttc
      661 accacaagcc gttttcgacg cgccacaact caccacggtt gccaaagagt ggccgctgtt
      721 ttcgtggtaa tggatgatga aaaaaataat tcccgccgta gtctgtttgt tgctttgtag
      781 ttgcacgacc tttccgcaag ctgtcgctcc ggtaaacggt gatgcctcca attagttgaa
      841 ttaaggatat aatactcgct tttaaggttg tttcatggtc acagttaacc ttcattgccc
      901 ccgttgccag tccgttcagg tttaccgtca cgggcagaac ccaaaaagac atgatcgatt
      961 ccgcggccgt gactgtcacc gggtatttca gctcagttac tgttacgagc cccgtaaacc
     1021 gggagttaaa gaccaaatcg ctgaaatgac tttcaacggt gccggggttc gcgatactgc
     1081 ccgtacgctg aaaatcggca ttaatacagt catccgaact ttaaaaaact ctcgccacga
     1141 agaataacgt catccccggt cgctcatgct gatgtggcac tcatctgcaa acttagtcag
     1201 caatggagct ttgtcggcag taaagcgcga cagcactggc tctggtacgt gtacaacacg
     1261 aaaactggtg gtgttctggc ctacactttt ggcccccgga ctgacgaaac ctgtcgtgaa
     1321 ttgcgggcgc tgcttaccct tttaccatcg gcatgttgac cactgacgaa tggggtagtt
     1381 acgctagaga gttaccgaag gagaagcatc tgactggtaa gatttttact cagcacattg
     1441 agcgtaacaa tctgaccctg agaacccgaa tcaaacgtct ggcccgcaga accatctgtt
     1501 tctcgcgttc tgtagaactt catgaaaaag tcatcggtgc ttttatcgaa aatatatgtt
     1561 ctactaattg gagtcatcac ccttctgata agctttcctt ggatgattac cagactgaat
     1621 acacagccaa agatttttac ctcattctgc tcttttttct caaatgacat atattatatt
     1681 ttacatcat
//
LOCUS       DAZFKM010000356         1687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFKM010000356 DAZFKM010000000
VERSION     DAZFKM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..202
                     /locus_tag="ACHKAY_005522"
     CDS             <1..202
                     /locus_tag="ACHKAY_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703955.1"
                     /translation="ELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFGSI
                     FLWVIIGVAPVAVAKMAWRFVS"
     gene            210..656
                     /locus_tag="ACHKAY_005523"
     CDS             210..656
                     /locus_tag="ACHKAY_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO5703956.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            653..1180
                     /locus_tag="ACHKAY_005524"
     CDS             653..1180
                     /locus_tag="ACHKAY_005524"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO5703957.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1177..1359
                     /locus_tag="ACHKAY_005525"
     CDS             1177..1359
                     /locus_tag="ACHKAY_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO5703958.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          459 a          349 c          464 g          415 t
ORIGIN      
        1 tgagttgatt ttttctgcat tgaggattct cggtgctatg tggatggtgg cgacgttcat
       61 tgtggttgcc agcagttttg tccggctggt aggcgaaggt aaagacctgg ttggtgtgct
      121 tttcggtagc attttcctgt gggtgattat cggtgttgcg cctgtcgctg tagcaaaaat
      181 ggcgtggcgt tttgtgagtt gaggtgacaa tgaagcaaat atacatgctt cgcaacgaag
      241 caatcagaaa caacgccata gacgcaatac tctcacttcc gatcgacgac aagtcacctc
      301 acgaagtcca cattaaagaa ccaaagcgga gcaatcctca aaaccgcctt atgtgggcgt
      361 tattgcagga cgtatcacgt caggtgcttt ggcatggaca gagacttgcg ccggaggact
      421 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgtgc
      481 ctggtatcga tggtggcgtt gtcatgcttg gcgtgcgtac cagcaaaatg cgaaaggcca
      541 gcatgactga gcttatcgaa atcatgtttt ggttcggctc agagcgcaac gtgcggtgga
      601 gtgatgactc ccggcgagag tatgaatggt cacaacgaaa aggtagggct gcatgactat
      661 caaatcaaat acaccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
      721 tgcgctggat attgagtttg gattctggct ggattcagct gcgagcgaca aaaatgctct
      781 gtgcgctcac tggctaactg aggccgacga cgcactcaat tctgagtgga taagccacgg
      841 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
      901 gcagtgcata caacagcgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
      961 atggttcagc aaggctctgg agagtgttga cgaagttcgc attatcactg atggacggat
     1021 taattttatc gaaccatcga cagggctgga gaagaaggga aacagtaaag gctccatgct
     1081 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     1141 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtttc
     1201 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctacag aaataaacgc
     1261 aagccaatcc caaaagaatc tgacgtaaaa accttcaatt acacggctca cctgtgggat
     1321 atccggtggc taagacatcg tgcgaggaaa acaaggtaat tgactaaaat cgaagttacg
     1381 aacaagaaag cgtcgagcgg gcttcagtgt acactgagtg gattctatct aggcttagtg
     1441 catacagaag attgctggta aaggacatgc caggcaaaac gatgaggact gatatttatg
     1501 aaaacatctg attttttact gttcttgcat gcggtacagg aggggctttg accgggcatt
     1561 ttatcgtgaa tattttcact tggtatttct ttggttttag agattacttc actcgatggg
     1621 ttttaaatag ttttcgtcgg tttatcgggt gcaagcctga tatgagaatt tataaagatg
     1681 aaaagaa
//
LOCUS       DAZFKM010000357         1682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFKM010000357 DAZFKM010000000
VERSION     DAZFKM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..103
                     /locus_tag="ACHKAY_005526"
                     /pseudo
     CDS             1..103
                     /locus_tag="ACHKAY_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2737 family protein"
     gene            100..207
                     /locus_tag="ACHKAY_005527"
                     /pseudo
     CDS             100..207
                     /locus_tag="ACHKAY_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            684..1352
                     /locus_tag="ACHKAY_005528"
     CDS             684..1352
                     /locus_tag="ACHKAY_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO5703959.1"
                     /translation="MSLIDYQALRAKAEKATCGVWSLEYGESRFDCDDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDELERNQQYIKRRDQE
                     NEDIALTVGKLRVELEGKDKLIAELGKQCAEWERKALSNFEECAAMAERIEEMSKQSC
                     EARERDLFESWVMHSICISKSTLEGLRTETGYRNATLSGTDFNRMWKQWKSIRATGIR
                     IKGE"
     gene            1354..1542
                     /locus_tag="ACHKAY_005529"
     CDS             1354..1542
                     /locus_tag="ACHKAY_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703960.1"
                     /translation="MSLTVRQLISKLRKMPPEAVVVWQGYDQSEGEYNDFVGHVADVT
                     DENAPSFDPEVRVVALRG"
     gene            1546..>1682
                     /locus_tag="ACHKAY_005530"
     CDS             1546..>1682
                     /locus_tag="ACHKAY_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703961.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     A"
BASE COUNT          500 a          335 c          466 g          381 t
ORIGIN      
        1 aaagccaatg ccatcgagag agtaattgct taagagaaag agtttcggtt ctgttaatga
       61 caacaaatat ctgaatgcta tgtggcggag tgggaaaaaa tgaaacaaat gtcactaatt
      121 gagatggatg gttttctgaa aggtaaatgc atcccacgag atctaaaggt taacgaaaca
      181 aacgctgaat atcttgtccg taagttcggt gaacttgaat caaaactgga aacggcgttg
      241 cgggagtgtc gttctgctgg aatcacgatt gataaccttg aggccaagtg cgcggcgctg
      301 gcagcggaga gtgcggggat gaagaagttc tgcaaagacg ctgcattcga tgccgattac
      361 gaagcagagc taggtatgga gcgtggttta ttcagtgatg cgcttaacga aatcaaaacc
      421 ccagccaccg atgctttcct ggctgaagta cgtgcggaag cacgcaacga gggtattaac
      481 tatgccgcaa gccgtcttgc tgctgctttc aaccacggat ttatcaataa gtctttacgt
      541 gaagttttcg atgttacgcg catgattctg tcagcgaaag aagagttggc taatgaaccg
      601 catccgattg atggcctgtc tggtgaatat gcggagaaat cccttgaaga atgggcggaa
      661 cagattcgca aaggaggcaa ccagtgagcc tgattgacta tcaggcactg cgtgccaagg
      721 cagaaaaagc aacgtgtggt gtatggtcgc tcgaatatgg agagagccga tttgattgtg
      781 atgatgcgtt aattcatcgt gaagttgttg gatatcttcc catttgcaga attgaaggag
      841 cacatccaga aagcggtttc gatgaagatt tccaaatgga acagcaggcc aatgctgaat
      901 tcatcgccgc agccaatccg gctactgtgc tggcattact ggatgaactg gaaagaaacc
      961 agcaatacat caaacgccgc gatcaggaga acgaggatat tgcgctaacg gtagggaaac
     1021 tgcgtgttga gctggaaggc aaagacaagc tgattgcaga gcttggaaaa caatgcgccg
     1081 aatgggagcg aaaagcatta agcaactttg aagagtgtgc tgcgatggct gaacgtatcg
     1141 aagagatgag taagcaaagt tgcgaagccc gggagcgtga tttgtttgaa tcatgggtaa
     1201 tgcattcaat ttgtatctcc aaatcgacgc ttgaaggatt gcgtaccgaa actggatacc
     1261 gtaacgcaac cttatcaggc acagacttca accgaatgtg gaaacaatgg aaatctatcc
     1321 gcgctactgg cattcgcatc aaaggagagt gagatgtcgc tgacggttag gcagttaatc
     1381 agcaagctca gaaaaatgcc tcctgaggct gtcgtggtct ggcagggcta tgaccaatca
     1441 gagggtgagt ataacgactt tgtaggtcat gttgctgatg ttacagatga gaatgcacca
     1501 tcttttgacc cagaagtgcg cgttgtggcg ctaagaggat aacccatgac cactattacc
     1561 aaagagcgac tactgacaat caagcagtgg cgcgaaacat acggacctgg tagcaacgtt
     1621 gtactgccag cagaagaagc ggaagaactg gcacgaattg ctctggcatc gctggaagca
     1681 ga
//
LOCUS       DAZFKM010000358         1676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFKM010000358 DAZFKM010000000
VERSION     DAZFKM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..616
                     /locus_tag="ACHKAY_005531"
     CDS             2..616
                     /locus_tag="ACHKAY_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO5703962.1"
                     /translation="MLRNEKTSYSLIKTEQTGKLLNRKITTTPTPAKLLSYRNADLIK
                     ENYITEKVLSIFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFG
                     IMMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSAS
                     VGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(629..850)
                     /locus_tag="ACHKAY_005532"
     CDS             complement(629..850)
                     /locus_tag="ACHKAY_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703963.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(934..1314)
                     /locus_tag="ACHKAY_005533"
     CDS             complement(934..1314)
                     /locus_tag="ACHKAY_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703964.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1515..>1676)
                     /locus_tag="ACHKAY_005534"
     CDS             complement(1515..>1676)
                     /locus_tag="ACHKAY_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5703965.1"
                     /translation="AVCTLFDVDALSRVVNDGSVHPLTRAPITPSMIVKPEECKYDPA
                     RGSFIIKDS"
BASE COUNT          571 a          351 c          279 g          475 t
ORIGIN      
        1 catattacgc aacgaaaaaa catcatattc tctaataaaa acagagcaaa caggaaaact
       61 gttaaacaga aaaataacca ccactcccac accagcgaaa ctattatcat atcgaaatgc
      121 agatttaata aaagaaaact acattacaga aaaagtttta tctattttca atataaaacg
      181 ggatttcgtt gctgtaagaa tccaaagtaa tcagtttact gatttaaaaa acaagacaat
      241 tcagggtcat aaagacactg tcgcaaaagt catagactgg tataacccac aaaaaaacgc
      301 atttggtatc atgatgggca caccaagacg ttctgctgat atagcaaagg aagaatccag
      361 aaacgctcta aatttcatga taatggaaaa ggatacattt aatgaaaaga tacttaacag
      421 caatgctaat ttacaaaaaa aatacggcac aacagaaaat agtagttggg tatcggcttc
      481 tgttggttct cttctggaca aaggagccaa agtatatcca gatatatcat gctctctgag
      541 actgggagaa ccattcatta ttacacttcc agaaacagtc aggctggatg ttaatatcca
      601 cccactcaaa aaatgatcct gaacgttttt atgcaggcaa atgcgtccac atcaaggctt
      661 caaccttgtg ctctccccct gtcacaggat aaatttgcct gtaaaaatca aactctccct
      721 gctcattacc aataccaatc agttgaatgc agttgccatc tacaataaat ttttccgctg
      781 acgtaaccaa aataaaggct tccggatgtt cattaggaaa ggtgttagca agagtacact
      841 gaatatccat ctccaacaaa atctcctgat tattagcgta aactcatcga cgaaatcgtt
      901 actatcccct gggcacatat gtaccattga atactactgt tctccagatt ctgataatcc
      961 atggcgaaca ataagaacac tacagccgct ttgctgatta gttagccata acggtgaact
     1021 ttgctggata gtttgagaaa tatcatttcg ccggaggctt atacactgat tactcaagct
     1081 tacaactgca ggtgcatcaa agcgctccgg actatcagta tcacgcatag ctgtaaacag
     1141 aatataatca ctaccagcca tatcatattc cgtttctttt agtcttaaca agccatcctc
     1201 tcttgtaata ttaactgtaa ttaaagatgt ctcatcatga tgggcatagc gctgtgtatt
     1261 aagaattaaa tgctcttcca gaaattttac agggttacta cgtatttcat tcattataca
     1321 ccgaagctct gaacgatgcg gcaccagaaa gtgcccggta ttttgtgaaa taatactact
     1381 taccttgatt gttttctctc cagctcatga aaaataactc gcgtattata ttgttttacc
     1441 aaccacaggc cccgaatgcc tgtggtgttt ttatataaat tatcggaagt taatattgtt
     1501 tcatttgaaa catcttaact atcttttata ataaagcttc cccttgcagg gtcatattta
     1561 cactcttctg gtttaacaat cattgatggg gttattggag ctcgtgtcag agggtgaact
     1621 gaaccgtcat taaccacgcg agaaagtgca tcaacatcaa ataaagtgca tactgc
//
LOCUS       DAZFKM010000359         1648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFKM010000359 DAZFKM010000000
VERSION     DAZFKM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..186
                     /locus_tag="ACHKAY_005535"
     CDS             <1..186
                     /locus_tag="ACHKAY_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5703966.1"
                     /translation="HKSGNLVPYRVELISRIGQEAVEEIESNHNRYRWTVEECRAIKA
                     EYQQKLKKLRNSRSEVA"
     gene            183..848
                     /locus_tag="ACHKAY_005536"
     CDS             183..848
                     /locus_tag="ACHKAY_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO5703967.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            845..1468
                     /locus_tag="ACHKAY_005537"
     CDS             845..1468
                     /locus_tag="ACHKAY_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5703968.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT          494 a          357 c          420 g          377 t
ORIGIN      
        1 cacaaaagcg gaaatctcgt tccgtatcgc gtcgaactga ttagccgcat cgggcaggaa
       61 gcagtagagg aaatcgaatc aaaccataac cgctatcgct ggactgtcga agagtgcagg
      121 gccatcaagg cggagtatca acagaaactt aaaaaactgc gaaacagcag aagtgaggtt
      181 gcatgaatat ctacgaaaga attgatggca gcaaataccg aaatatttgg gtagttggcg
      241 atctgcacgg atgctacacg aacctgatga aaaaactgga gacgatagga ttcgacacca
      301 aaaaagacct gcttatctcg gtgggcgatt tggttgatcg cggtacagag aacgtagaat
      361 gcctggaatt aatcacattc ccctggttca gagctgtacg tggaaaccat gagcaaatga
      421 tgattgatgg cttatcagag cgtggaaacg tcaatcactg gctgcttaat ggcggtggct
      481 ggttctttaa tctcgattac gacaaagaaa ttctggctaa agctcttgcc cataaagcag
      541 atgaacttcc gttaatcatc gaactggtga gtaaaggaaa aaaatatgtc atctgccacg
      601 ccgattatcc ttgtgacgaa tacgagtttg gaaagccagt tgatcatcag caggtaatct
      661 ggaaccgcga acgaatcagc aactcacaag acgggatcgt gaaagaaatc aaaggcgcgg
      721 acacgttcat ctttggtcat acgccagcag tgaaaccact caaatttgcc aaccagatgt
      781 atatcgatac tggcgcagtg ttctgcggaa acctcacatt gattcaggta cagggagaag
      841 gcgcatgaga ctcgaaagcg tagctaaatt tcattcgcca aaaagcccga tgatgagcga
      901 ttcaccacgg gctacggctt ctgactctct ttctggtact gatgtgatgg ctgctatggg
      961 gatggcgcaa tcacaagccg gattcggaat ggctgcattc tgcggtaagc atgaactcag
     1021 ccagaacgac aaacaaaagg ctatcaacta tctgatgcaa tttgcacaca aggtatcggg
     1081 gaaataccgt ggtgtggcaa agctcgaagg aaatactaag gcaaaggtac tgcaagtgct
     1141 cgcaacattc gcttatgcgg attattgccg tagtgccgct acgccgggcg caagatgcag
     1201 agattgccac ggtacaggcc gtgcggttga tatagccaaa acagagcagt gggggatagt
     1261 tgctgagaaa gagtgcggaa gatgtaaagg cgtcggttat tcaagaatgc cagcaagcgc
     1321 cgcatatcgc gctgtgacga tgctaatccc aaaccttacc caacccacct ggtcacgcac
     1381 tgttaagccg ctgtatgacg ctctggttgt gcaatgccac aaggaagagt caatcgcaga
     1441 caacattttg aatgcgatca cacgttagcg ccatgattgc cacggatggc aacatattaa
     1501 cggcataata ttgacttttt gaataacttt ggggaaactt gacaccaata atgggcgttt
     1561 tttacatgtc attgatgagt ctcaataacc tgccgccgag tagtttttat gctctgaatt
     1621 gtatttgtgt agtaaacatg ctgactgc
//
LOCUS       DAZFKM010000360         1640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFKM010000360 DAZFKM010000000
VERSION     DAZFKM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.363"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..525
                     /locus_tag="ACHKAY_005538"
     CDS             <1..525
                     /locus_tag="ACHKAY_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5703969.1"
                     /translation="LPWLSEADRRLQIKSALSWWLVCRGDIHKFRCVPHLTGRLFEHG
                     VTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEATGFYRISLPSAQPGDI
                     LLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHVSAF
                     TGIYNDLAAASACM"
     gene            522..1103
                     /locus_tag="ACHKAY_005539"
     CDS             522..1103
                     /locus_tag="ACHKAY_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5703970.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKMVVIGRQA"
     gene            1342..>1640
                     /locus_tag="ACHKAY_005540"
     CDS             1342..>1640
                     /locus_tag="ACHKAY_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703971.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPV"
BASE COUNT          377 a          404 c          521 g          338 t
ORIGIN      
        1 ctgccctggc tgagcgaggc cgaccggcgg ctgcagataa aaagtgcact gtcctggtgg
       61 ctggtctgcc ggggggacat tcataaattc cgctgtgtgc cacatctgac aggacggctc
      121 tttgagcacg gggtgacgga ctgttacacc ctgttccggg atgcatacca tctggcggga
      181 attgatatgc cggattttga gcgtgaggat gactggtggc gcaatggtca gaacctgtac
      241 ctggacaata tggaggcgac tggtttttac aggatttccc tgccttccgc acagcctggc
      301 gatatcctgc tgtgctgctt tggcgcatcg gtggccaatc atgccgccat atactgcggc
      361 aacggtgaac tgcttcacca tctgcctgaa caactgagta aacgggagag gtattctgaa
      421 aaatggcaac gacgaacgca ttctgtctgg cgtcaccgcc actggcacgt atctgccttc
      481 acggggattt acaacgattt ggccgccgcc tcagcctgta tgtgaacacg gcagcggaag
      541 ccatccgggc gctgtcgtta caggtgccgg gattccgccg tcagatgaac gaaggctggt
      601 accagatacg tattcgcggt gaggacacgg caccggaggc ggtgtacgcc cgtcttcacg
      661 aacctctggg tgagggaacg gtcatccaca ttgtgccgcg actggccggg gccggaaagg
      721 gtggactgca gattgtgctg ggggcggcgg ccatcgtggg gtcgttcttc acggccgggg
      781 catcaatggc gttatggggt tcagccctgg cagccggtgg tttttctgcc accacgatgc
      841 tgttttcact gggggccagc atgatactgg gcggtgtggc ccagatgctt gccccgaagc
      901 caaaaacacc ggaatacagg gcaacggata acggcagaca gaacacgtac ttttcctcgc
      961 tggataacat gattgcccag gggaacccga tgccggtgcc ttacggtgaa atgctggttg
     1021 gctcccggcg aatctcccag gacatcagta cccgtgatga aggcggtgac gggaagatgg
     1081 tggttatcgg gcggcaggca taaaagcgaa aaaatcccgc agtgctcacg gacaggaact
     1141 gcgggagcgt tacgaagatt gagtgtaagg aattattctt atgtcacgac aaaaaacatt
     1201 aacgcagaga aattattagc gccacagtca gtttgtgaaa atgtgaagat attcagaatt
     1261 tttattcagt catgatacag gcatcctccg ggatgcctgt tgtttttgtg cgtaacagtt
     1321 atcacagtaa agggtgagac aatgggcaaa ggtggcggca aggcgcacac gccggtagag
     1381 gcaaaggaca atcttaagtc cacgcagatg atgagcgtga ttgatgccat tggtgaaggg
     1441 ccgattgaag gcccggtgaa ggggctgcag agtatcctgg tgaacaaaac cccgctgacg
     1501 gacacggacg gcaatcctgt gatacacggt gtgaccgcgg tctggcgcgc cggggagcag
     1561 gagcagacac caccggaagg ctttgagtcc tccggagctg aaaccggact gggcgtggaa
     1621 gtgacgaagg caaagccggt
//
LOCUS       DAZFKM010000361         1626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFKM010000361 DAZFKM010000000
VERSION     DAZFKM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.364"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1614
                     /gene="tir"
                     /locus_tag="ACHKAY_005541"
     CDS             <1..1614
                     /gene="tir"
                     /locus_tag="ACHKAY_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO5703972.1"
                     /translation="PIGNLGHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMGS
                     RLLFTPIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNSA
                     IGSSLFRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRGG
                     AGHAMVTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLRA
                     DPKLWLSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLTK
                     EAFQNPDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQK
                     YDEQHAKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVVD
                     NQPTNNASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRND
                     SLARISEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQGIQSTYALLASSGGLR
                     LGMGGLTGGGESAVSTANAAPTPGPARFV"
BASE COUNT          445 a          373 c          430 g          378 t
ORIGIN      
        1 cctattggta atcttggcca caatcccaat gtgagagctt taattccacc tgcaccgcca
       61 ttaccttcac aaaccgacgg tgcaggaggt gcccgtaatc agctcattaa ctcaaatggc
      121 ccgatggggt ctcgtttgct atttacgcct ataaggaatt ctgttgctga tgctgctgat
      181 tctcgtgcca gtgatattcc cggacttcct acaaatccac tgcgctttgc tgcgtccgag
      241 gtatctttgc atggtgcgct tgaagttctt catgataaag gggggcttga tactcttaac
      301 tctgctattg gatcttcgtt attccgtgtt gaaactcggg atgatggcag ccatgttgct
      361 atcgggcaaa aaaatggcct cgagaccact gttgttttaa gtgagcaaga gttttctagc
      421 ttacagtccc ttgatcctga aggtaaaaac aaatttgtat ttactggagg ccgcggtggc
      481 gcagggcatg ctatggtcac ggttgcttca gatatcgccg aagcccgtca gaggataata
      541 gataaattag aaccaaagga tacaaaggag acgaaggagc caggggatcc aaatagtggc
      601 gagggaaaaa tcattgaaat tcatacctca acctcaactt ctagcctccg tgcagatcct
      661 aaactttggt tgtcattggg gactattgct gcaggtctga tagggatggc tgcgacgggg
      721 attgcacagg ctgttgcgtt gactccagag ccggatgacc caatcactac cgaccctgat
      781 gctgcagcaa acacagctga agcagcggca aaagatcagt taacgaaaga agcattccag
      841 aacccagata accagaaagt taatatcgat gagaacggaa atgcaattcc gtccggggaa
      901 ctaaaagatg atgttgttgc gcaaatagca gaacaagcta aagcggcggg tgaacaggcc
      961 agacaggaag ctattgaaag taattctcag gcgcagcaaa aatatgatga acagcatgct
     1021 aaacgcgaac aggaaatgtc tctttcatcg ggggttggct acggtattag tggtgcgctg
     1081 attcttggcg ggggaattgg tgccggtgtt actgctgctc ttcatcggaa aaaccaaccg
     1141 gcagaacaaa caatcactac acgtacggta gtcgataatc agcctacgaa taacgcatct
     1201 gcgcagggca atactgacac aagtgggcca gaagagtccc cggcgagcag acgtaattcg
     1261 aatgccagcc tcgcatcgaa cgggtctgac acctccagca cgggcacggt agagaatccg
     1321 tatgctgacg ttggaatgcc cagaaatgat tcactggctc gcatttcaga ggaacctatt
     1381 tatgatgagg tcgctgcaga tcctaattat agcgtcattc aacatttttc agggaacagc
     1441 ccagttaccg gaaggttagt gggaacccca gggcaaggta tccaaagtac ttatgcgctt
     1501 ctggcaagca gcggcggatt gcgtttaggt atgggaggat taacgggggg gggcgagagc
     1561 gcagtaagta ctgccaatgc cgcaccaacg ccgggacccg cacgtttcgt ttaaatatat
     1621 ccgtga
//
LOCUS       DAZFKM010000362         1613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFKM010000362 DAZFKM010000000
VERSION     DAZFKM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1364)
                     /locus_tag="ACHKAY_005542"
     CDS             complement(<1..1364)
                     /locus_tag="ACHKAY_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5703973.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEG"
     gene            complement(1351..1506)
                     /locus_tag="ACHKAY_005543"
     CDS             complement(1351..1506)
                     /locus_tag="ACHKAY_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO5703974.1"
                     /translation="MCFEHIEDFVADIAVIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(1515..>1613)
                     /locus_tag="ACHKAY_005544"
     CDS             complement(1515..>1613)
                     /locus_tag="ACHKAY_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703975.1"
                     /translation="MASRDFIQLCVVAVNFLSGADSGGKNEQATEA"
BASE COUNT          316 a          511 c          418 g          368 t
ORIGIN      
        1 ccttcaagtt cttttctcag tttgggtaag gagccgtcac ctgccgcatc aacgagggcc
       61 ataaatgatg tgaaagcctc ttctccggca atgtccttaa agaacgatac ccggtcaact
      121 tccccgtatt tgcgggtagc tttataaagg tcagccagca catcctccat cgggcgcatt
      181 ttgcccccgg catccgagac agacacgcca agctctttca gcgcctctgc tgccgccttt
      241 ggcggtgatg ccagacgagc caggctggca cgcattgccg taccagcatc actccccctg
      301 atacccatat tcgccagcac gcccgccatc gctgcggcct gctccagcga tattcccagc
      361 ttacccgcca ccggacctgc atatttcatg gtttcaccca gtgcgcgaag gtcagtgttg
      421 gtacgggtaa acgctgcggt gagtgtgtcg ccgacccggt ccatctggtc agcagaaaga
      481 ccgaactgcg tcagaatatt tgagccaata tccgccgtct caccgaggtc cataccgcca
      541 gccgttgcca tgctcagtac gccagggagc gcagcctgaa tggcctgtgg tgtgaagcca
      601 gccattgcaa gaaatgcctg tccactggcg gcatcgcctg cggtgaactg cgtttcagag
      661 ccaagtttta acgcctgctc acgcagcgcc ttaaactgcg ggctgttctg gtcgattcgc
      721 gtcagtgcct gaacgcggga catctctttg ccgaacccga tcgcaggctg caaaaaacgc
      781 ccggcagcat agccgcccgc cgctgccgca ccaattgcca gcgcaccacc tgttttcagt
      841 tttcccgcgg tttcctgcgc gcgcgaatac cgctcacgcg cccgcgttac acgcgcaagc
      901 gcctgccgtt cgcgttcaag ctggttgttg tactgttcgg tgcgtctgat ggcctgctgg
      961 atggtgttat cgctgcctgt cagggaaatg ccgtggcgtt tcagctctcc gccaagctcc
     1021 cgcattttct gaatttcccg cgtgcgcgat tcattcaggc gttcaagccg ggtgcttaac
     1081 tgctgcatca gcttttgttg tttttcgctg agcactgtac ccgtgcgttg taactgatta
     1141 agggcgttaa gctggcgtcg tgctttcacg atgccagcat ccgctttact gacagcgtca
     1201 cgggcgcgct caaatgaacg cgcctgacgc tcgagatttt tgatcgcccc ctgcgttcgc
     1261 tggatggagt caccaaactg ccccatcagg cggcgggcgt tttcggcagg ccgggtcagc
     1321 ctgtcaacgg cgctgaaagc gacccggata tcaagagtct tcattgtctg cattcccgct
     1381 gcgaagtgcc gcccgctcac gccagctaac cacttcgccg ggcgtcatca tgaagatttc
     1441 ggcgggcgac cagttaaaaa taacggcaat atctgccaca aagtcttcta tgtgctcaaa
     1501 gcacacaacc gtgatcaggc ttccgtcgcc tgttcgttct tcccgccaga gtccgcaccg
     1561 ctcaaaaaat ttacggcaac cacacataac tgaataaagt cacgggatgc cat
//
LOCUS       DAZFKM010000363         1583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFKM010000363 DAZFKM010000000
VERSION     DAZFKM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..290
                     /gene="ygeI"
                     /locus_tag="ACHKAY_005545"
     CDS             72..290
                     /gene="ygeI"
                     /locus_tag="ACHKAY_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO5703976.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            373..875
                     /locus_tag="ACHKAY_005546"
                     /pseudo
     CDS             373..875
                     /locus_tag="ACHKAY_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(894..1526)
                     /locus_tag="ACHKAY_005547"
                     /pseudo
     CDS             complement(894..1526)
                     /locus_tag="ACHKAY_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          545 a          300 c          241 g          497 t
ORIGIN      
        1 atttaaaatg ctctagagtt aaaagtatga tgtaaagacc attggcctgg acaattaaaa
       61 aagggagctt tatgactaat ccaatcggta tcaataattt atctcaaagt tcaaatatag
      121 cgaatgcaac gggtgatgaa gtcgtatcat tagataaaca catcaacacc agtgctactg
      181 atacagatca aatacaagca tttatagttt caacatggat ggcgcctttt caaaatgata
      241 tgtattcaga agataatcct atctcacctt actataaaat tgaatggtag acgattgacc
      301 aaataaatca taacgagata cttttataat agtatctcgt ccctttcatt caatcctcac
      361 acatgaaaaa atatgcgctc accaaaagtt aaattcttaa ccatcttcac gttttgtata
      421 tttattacaa aaatgtcttt cgccagcaac tcttgctcta atgaagcagg aacaatgttc
      481 agaatcgaac caaatttaat taaagctatc gctcttgttg aaagcaatct aaaaaaggat
      541 agcataggca agaacagaga taagaataac aatataaaga gtttagatta ttggttaatg
      601 cagataaacc agatgcatat tcccctgtta aaaaaacgtg gaataataaa agatgaacga
      661 gatttgttag ataacccttg tctgaacatt aaaataggaa ccgagatttt atataaccat
      721 ttttcacgtt gtggagtaac gtggcaatgc ctggggactt ataatgcggg ttttgcaatg
      781 gataaccaaa aaaagagaca gcaatatgcc ccaaaatata cattgtatat accaggctta
      841 atgaactaga caatcgtaag gcttcagcaa aatgagccta aagcctcttt tttttatata
      901 gtgcaaacac ccatacgtaa agctgttttg atcaactcga taccactgtg aacatctaat
      961 ttcttcatga tattgagtct atgagtttcg actgtttttc tgcttaactg caagaaatta
     1021 gcgatttctt tgtttgtttt accatcagct atgagttgta atatttctga ctcacgattt
     1081 gtaagttgat tatcagagga atacctggaa gatatttttt cacaatccat atgtacacta
     1141 tcaaaataag tgtaaccatt actaatcgaa tcaatagcac gatttagttt cgccgggtga
     1201 ctggttttcc atacgcatcc tttcgcacct gcttcaagca attttattga atcaatatac
     1261 ggcatctttg ctacaaatgt aataatatga gcatctatct tatgcgattt aacccatttt
     1321 acgagttcgg accccatcat tccacctgca aattctccta aaaaaaatat ttaggctgta
     1381 gatttgttac actccgctat agctttcttc agatctttat agcccccaac aacctcatat
     1441 aaatcaggat aatgtccgag aaatccaatt atcccatcaa tcataaacgg ctgctgatct
     1501 gaaactacaa ttttaatttt tcccatatat tcatcctgag tgtaaatgct ttaacgcatt
     1561 catttataat catcccttcc att
//
LOCUS       DAZFKM010000364         1579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFKM010000364 DAZFKM010000000
VERSION     DAZFKM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..996
                     /locus_tag="ACHKAY_005548"
     CDS             <1..996
                     /locus_tag="ACHKAY_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO5703977.1"
                     /translation="IVSRDRSGVYATAAREGAPQARQVADRWHLLKNIGDALERMMYR
                     HIPLIRLVASELSPKKSPDPEPSVPAASLRRPERLKQQTRKKRHQRWTEVMALHNKEC
                     SFREISRITGLSRVTVSRWVRSGTFPEMSTRPPKRGLLDPWREWLKEQRESSNYNASR
                     IWREMVARGFTGSETIVRDAVAKWRKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEE
                     NYASRFISLMCEKEPELKIAQQLALEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVA
                     AGMEADAAAICEAISSRWSNGVVEGHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            993..>1579
                     /locus_tag="ACHKAY_005549"
     CDS             993..>1579
                     /locus_tag="ACHKAY_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO5703978.1"
                     /translation="MKISTQVAEEPLSVVDTDDIFLLKQRLAEQEALIHALQEKLSNR
                     EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLHKESDTLAGRVYDPAVQR
                     PLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTTEQLELMRSAFRVIR
                     TVREKHACTQCDAIVQAPAPSRPIERGIARPGLLAR"
BASE COUNT          407 a          402 c          450 g          320 t
ORIGIN      
        1 attgtctcgc gtgatcgcag tggagtctat gcgacagcag cacgtgaagg tgcacctcag
       61 gccagacagg tggccgatcg atggcatctg ctaaaaaata ttggcgatgc gcttgaacga
      121 atgatgtaca gacatatacc tctgatacgt cttgttgcca gtgagttgtc accaaagaaa
      181 tcacctgatc cagaaccatc tgtgcctgca gcatcgctcc gtcgtccgga acgcctaaaa
      241 caacaaaccc gcaaaaaacg gcatcagcgt tggacagagg ttatggccct gcataacaag
      301 gaatgtagtt tcagggaaat atcccgtatt acaggcctgt cgcgtgtgac agtcagtcgc
      361 tgggtgcgtt caggaacatt ccctgaaatg tcaacccgac ctccaaagcg agggcttctg
      421 gacccatgga gggagtggtt aaaagagcaa cgagaaagta gtaattataa cgccagccgg
      481 atatggcggg aaatggtggc ccggggattt acaggcagtg aaaccatcgt cagggatgcg
      541 gttgccaaat ggcgtaaagg ctggaaccca ccggttacta ctgccgtcag acttccttca
      601 gtgtcccggg taagccgctg gttgatgccc tggagaataa ccagggatga agaaaattat
      661 gcttcccgat ttattagtct gatgtgtgaa aaagaacctg agctgaaaat agcgcagcaa
      721 ctggcactcg agttctaccg tattctgaaa acccaaaata aatcacagct aagcagttgg
      781 ttcaatcgag tccacgaaag cggttcagca gaatttcggc gcgtggcggc ggggatggaa
      841 gctgatgctg cagctatatg tgaggcaatc agtagtcgct ggagtaatgg tgttgtcgaa
      901 ggtcatgtaa atcgcctgaa gatgctgaaa cgccagatgt atggtcgagc aggatttgaa
      961 ctactcaggc agagggtaat gagtccactg gcatgaaaat ctccacccag gttgcggaag
     1021 agccactttc cgtcgttgac actgacgaca tcttcctgct gaaacagcgc ctggccgaac
     1081 aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc
     1141 atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag
     1201 tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcataaa gagagcgata
     1261 cgctggctgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag acccgcaccc
     1321 gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct gcggcgccgt
     1381 gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccacc gaacagctgg
     1441 agttgatgcg tagcgccttt cgggttatcc ggacggtacg ggaaaaacat gcctgtactc
     1501 agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg ggtatcgcca
     1561 gaccggggct gctggcccg
//
LOCUS       DAZFKM010000365         1574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFKM010000365 DAZFKM010000000
VERSION     DAZFKM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..354)
                     /locus_tag="ACHKAY_005550"
     CDS             complement(<1..354)
                     /locus_tag="ACHKAY_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703979.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEV"
     gene            complement(645..1220)
                     /locus_tag="ACHKAY_005551"
     CDS             complement(645..1220)
                     /locus_tag="ACHKAY_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO5703980.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            complement(1333..1572)
                     /locus_tag="ACHKAY_005552"
     CDS             complement(1333..1572)
                     /locus_tag="ACHKAY_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5703981.1"
                     /translation="MCGCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          435 a          440 c          268 g          431 t
ORIGIN      
        1 cacctcacca ggacagtgaa gtttttcaat atcaacagta aaggtacatt ccgatatttt
       61 ggcctgcaaa gagttctctt gtcgctgcgc caccattcca ggaagcacgc ttaacaccct
      121 gtttctgagc gcatcaactc catcattaaa accaatattc aataattctg cataaaccag
      181 tcccagccct tcactttctg ctaaaaaacg gcctgtatct ggcatataag aaactctaac
      241 tctctcccct cccaggagaa atcctccaat ctgcccttcc attacctgct gacgcatttc
      301 cgccatctca ttcgcgaatg aagtcgatga tatatatgat actaatccag gcattaccat
      361 attccttcat acagataaat atttcctctg ggcttcataa caaacatctc cctgacatga
      421 caacaaaaac cggagccgga ctccggtttt tgtgaatccg tcggctattt catcccgcca
      481 atatttcccc gtcagcacgc cagatttgca gcggcctcac cactcactgt atatgctttt
      541 tagctgcatc cagtacacca attaccacat ccttataccc tgaacatctg tagcatttca
      601 tataagcaac tggcagttaa catatagctt gggtaatatt tatttcactt atcctttatg
      661 acaaagtttc ctttggtttg gtcataaata cattgttctt gacttacaat cattgatgac
      721 gttattggtt ctcgtgttag cgggtgcttc ccaccgtcgc gaaccaaatg agaaaaagaa
      781 accgaatcaa ataaggtaca aaccaaagaa ccttctgaat ttttaacaaa cacaccttct
      841 tctggtttat ccagtataat tgggcatcga accgcctcgg atggacactg aaggctctcc
      901 gtattaacag aaaacttaca ttgtgatatt ttcccctcaa tatcaccatg tgattgtgtg
      961 ttacctgaac ctgaaaccat tcttaacatt atatccctga gcgcttcagg accaccatta
     1021 agccctgtaa gtaataatcc agaaagcaaa ctgttattgc ccccactcgc cagaaagcga
     1081 ccatccgttt cactatatgt gatacggacc gtctgcccgc ctactgtaat ttctccaccg
     1141 ccactggcgg ccatttgagc acgaagaacc tgcattccgg tggaaaatga attggatgca
     1201 atagctgatg ttaacggcat gacaaaactc cctatttaaa tattcattcc aaacacaggg
     1261 agtcataaca aacagccccc atacatgaca acaaaaaccg gagccggact ccggttttgt
     1321 gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag cacgcagaat
     1381 ttgcagcggt cttaccacac actgtatctg ctttttatcc gcatccagta tcaccacctg
     1441 cgtgattacc ctgtcctgct ccgggataat accattctca tcggactcca ggatgtctgc
     1501 cggccccagt cgcagttgtg ctgtaagcga ctgcacgtgt tcacggccat catgctttcc
     1561 gcaaccacac agac
//
LOCUS       DAZFKM010000366         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFKM010000366 DAZFKM010000000
VERSION     DAZFKM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..908)
                     /locus_tag="ACHKAY_005553"
     CDS             complement(<1..908)
                     /locus_tag="ACHKAY_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5703982.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHSDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPS"
     gene            complement(892..1248)
                     /locus_tag="ACHKAY_005554"
     CDS             complement(892..1248)
                     /locus_tag="ACHKAY_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5703983.1"
                     /translation="MARPPKAPAYLNDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(1371..1553)
                     /locus_tag="ACHKAY_005555"
     CDS             complement(1371..1553)
                     /locus_tag="ACHKAY_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703984.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
BASE COUNT          315 a          439 c          399 g          420 t
ORIGIN      
        1 tgcgacggaa taccccgcgc tttctggatg gttgaggcca gtttttcacg atccacggaa
       61 acatcgatgt tagggttagc ctttatccac atcgccggat catcaacctc gttttcgtcg
      121 tccagctcgt aaatcagcac aaaaattgaa tcgttgacct cttcgccgtc caggatctgg
      181 cagcaataat catagtgctg tttacaggct gaaacgacgt tactgcccga tgtggtaatg
      241 gcaaataaca gcccttcggg acgtgcgccc atccccagct caagcgcgga ataaacgccg
      301 ttatcggagt gtaggtggta ttcgtccacg atggaaaggc ttgggtttgt gccctcgatg
      361 gttgccgctt ttgctgccag cggctttaac aggctgttgc ttttcgggtg catcaccttg
      421 tgggcctgaa tattcacccg cctgcgtaac ggtcgggata aaaggcacat ctgacgcgca
      481 tcatcaaaaa cgatccgcgc ctggtcacgg ctcaccgctg cggtgtaaat atcctgctgc
      541 ccgttctcca taatcagaaa ccagttagcc agaatcgcgg cggtcgtgga tttcgcattt
      601 ttgcgcggca cttcgataaa ggcgctcgtg tatttgcgcc gtccggtggc cttaacctta
      661 aagccgagga tgcacgcaaa ggcgaactgc tgccacggct ccagctcaat gggtctgcca
      721 cgcatcggcc cttttacgtg cgggcacacc ctggaaaagg caataaaccg ctccacaacc
      781 tcacgatcga acgtgtaaag ggggctttca aggtccgaaa agtaccgttt aacggcctgt
      841 ttcagccgtt tacaggccgg aattttgccc gtttttacgt cttctgcgta cttattccag
      901 gcggtcaagc tcgtcctctt cttctgtttc cggtggattt ttacggcggc ttatcgggtc
      961 aaaaccgagc aaggaggcca tttttatcat cactctttca gcgtcggatt ttgcgcttaa
     1021 tgcggggttt ctgctctcgc caccctgact gttaacaatg ctgaacccgc gcgccgcaag
     1081 gtctgcgacg gctttccggt atatcgagta attgacgcaa tacagttcca gattactcca
     1141 gtcggcgggg gtaaggtctc cccgttccgc aagctgccgc gatttttccc gccactgctt
     1201 cacggcgatg tcattcaggt aggcgggggc tttcggtggt cttgccatgc ttatttttcg
     1261 ccagattatt tttcaaaaaa ttcccgtgca taaaaatttg aggaggcgtt cggtgcccgg
     1321 cggggtcggg tttgtcctga aaacgccccc caccccgtca tacagcctca tcagcgattg
     1381 cggaaacatt ccataacctc gcggtcacgg tcggttaatc gcttcgctgt gatgcgttct
     1441 gcgcgtcctg acgctttatc tttatgccct gtttcctgtg tcttccatgc gtcacgctgc
     1501 cttataagtc cacggataag gcggttttgt tcccgctcat tcatcagcgt catacatcca
     1561 gttattgcgg tta
//
LOCUS       DAZFKM010000367         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFKM010000367 DAZFKM010000000
VERSION     DAZFKM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.370"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHKAY_005556"
     CDS             <1..261
                     /locus_tag="ACHKAY_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFO5703985.1"
                     /translation="ENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLI
                     AAGLESNLTVYTGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(403..1527)
                     /locus_tag="ACHKAY_005557"
     CDS             complement(403..1527)
                     /locus_tag="ACHKAY_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO5703986.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          466 a          360 c          290 g          456 t
ORIGIN      
        1 gagaataata cctcctgggt aagtgagcag atactttcag cgcctccgaa agatgtaaat
       61 attcgacttg gagtcggttc tttagaaggt acaaccgtct cacatgttca gcgattgcat
      121 cattcgttaa tcgcagctgg tttggaaagt aacctcactg tctataccgg tggtcacgat
      181 tatgcctggt ggcgcggagc aattattgat gaattagcaa attataattg caggaaggta
      241 tcaaataata actctgtgta aaatatcgtt ttatcgtcag ataactcttt ctgcaatatt
      301 attatgggag actatctcat ttatttagtg atgtccttca tttatgtgat gaaggacatt
      361 atattactca taacatttct cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt
      421 gttttttaat tttctcatca aaacctctgc aatctctgca ggactgggtt gttcagccat
      481 ttcagcggct acctgatctg aacagaagcg tagtgaatca tcataaagaa ggcgatttac
      541 caaatcactg gtcagtccat gcttgtccgg aataatccca cacccacgct tcgcaacaat
      601 ttctgcatta acagagcgat ctgcaccttc gccaaacaca atctgtggta ttccactata
      661 cagcgctgtc agggtatttc ctgcgccacc gtgatgaata aatccatcag ctccattaag
      721 gaatacaccc ataggtatcc agtcaaccag acgtacattt gatggtagtt ttcgtaaacc
      781 agtacgagca tttattgcaa gttgcaaaat gatatcagca tcaacttcat ttgcagaatc
      841 cataacccat gaaatcagct ccagaccatc aaccattggt tttacagtgc caaggctgat
      901 aagtaaacgt ttccgatcag aatccctgtc ccaccatgtt tcctttacag cacctccgtt
      961 ataaggaata tatctcattg agataaccgg ttctccagca tttttaagaa tgctcatact
     1021 tgggggagca acatcaatcc atgctaaatc acatagtgga ccgctgactc catgccgcct
     1081 gtaagcattg cttaaagaac ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc
     1141 aacagccagc atcactgaag gaattctata tttagcagca accaatgggc ctgccggatc
     1201 aagaggggga tagactatta aatctggcct ccattttcct gcaaaatcga tgaggttatc
     1261 tgccatttca tcgctaaaaa aagagaaatg accaataata ttactttttt tcctcaactc
     1321 ttcctgatgg cgatatccag cctctgaatc taaacctgga actgcatcaa aaacaaccag
     1381 tccagcttct gctgctttat tcgcgaattt gccagcgcta ctaattacta catcatgtcc
     1441 aattacacga aaggcctgag ccagagaaat caatgggtat aacaaaccgt acagcggtgg
     1501 gccaataaaa agaatacgtt ttcgcattaa aaaagtccgt cttctgctta cctgtttatt
     1561 tttctatctc ta
//
LOCUS       DAZFKM010000368         1552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFKM010000368 DAZFKM010000000
VERSION     DAZFKM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.371"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(924..1400)
                     /locus_tag="ACHKAY_005558"
     CDS             complement(924..1400)
                     /locus_tag="ACHKAY_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5703987.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          345 a          412 c          393 g          402 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctcctgcacg ttttttgccg tccgttcacc gttaaccccc
      241 gcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccatctggtc agggcttcgc cccgacaccc cgtatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactaac gttcggtcgc tggggctgcg
      421 gcgagcggtg ccacctctcg catgttgtcg gtgattccgc tgttgttctt tcggggatga
      481 gtcgtcagat agcccttcaa acgcgctcag aagctctcag acgcacgagg cggggtttaa
      541 atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agcgtttcag gaggtcaggc
      601 gcgccgatac ccctagccag gggctttcag gtcgccagga accgcgccag ccccctgcaa
      661 agcccatttg cacggtacag gcagtcactc ggttggtagg tggtcgtaga ccgtcggtgt
      721 tacagcgatg ggggtctggg gttctcccca gacggtttcc ggttgcgctc aaggcgctgc
      781 cggaaccgtg gcggctcggt cgttctggag atcggggagc aaaaacacgc aagggatttt
      841 gaggcggcaa aagagcaatg caggatagac aatcatggtc agaacgttaa cacgtagccc
      901 ctgattgtcc gcctgccgct ggctcaattt gaaaacatgg ccatgattcg gcgctgaata
      961 tcaccgtctt tgttgaccag tgttttagtg acaaatgctg catggttttt cgtagtgttg
     1021 tatcccttcc agacttcatg tggatcgagg gcatacgcac aaaccttacc agcaacgggg
     1081 attctaacta atgcattttt tgattctaag taattgagcc aacgaataat tgtgcttctg
     1141 ctaactccaa gtttttcagc taggaattgc tgatccgcaa cgacagcgcc acacgtagga
     1201 tcgatatgct ccgctaaaaa tgagtacagc ttcagtgctg atatgccttg gctatccttc
     1261 agcagctctc gaacgcgttc ccaacctttt ggggatacct gtgtaaacct tgaattcttc
     1321 tttgcttctt cgagatcctc ggtgtttttc tttgcctcat ctgaaagttg tttcagtcgt
     1381 ttttctgaat caataaacat gattttttcc ctctaaaaat cccatgcact aacgttggtt
     1441 caaatctaag ccatcatgac acatctgtgc aacaccttgt tgcgtgttgt cgcatccagg
     1501 atgcgacaac gttgtgtcac cctggatgcg acaggtaaac aaaaaacaca ct
//
LOCUS       DAZFKM010000369         1522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFKM010000369 DAZFKM010000000
VERSION     DAZFKM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..354
                     /locus_tag="ACHKAY_005559"
     CDS             <1..354
                     /locus_tag="ACHKAY_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5703988.1"
                     /translation="IEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVRQQRL
                     KLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVRGQLV
                     PGGRLGIKITELECA"
     gene            351..1040
                     /locus_tag="ACHKAY_005560"
     CDS             351..1040
                     /locus_tag="ACHKAY_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5703989.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            1236..1311
                     /locus_tag="ACHKAY_005561"
     tRNA            1236..1311
                     /locus_tag="ACHKAY_005561"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1269..1271,aa:Ile,seq:cat)
     gene            1402..1475
                     /locus_tag="ACHKAY_005562"
     tRNA            1402..1475
                     /locus_tag="ACHKAY_005562"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1436..1438,aa:Arg,seq:tct)
BASE COUNT          405 a          326 c          407 g          384 t
ORIGIN      
        1 atagaatttg ttttgcctta cccgccgacg gtgaatacct actggcgacg tcatggcaat
       61 acgtatttca tctcggaagc cggaaagcgt tatcgccgtg atgtggcgct aattgttcgc
      121 cagcagcggc tgaaattaaa cctgtccgga aggctggtga taaagattat tgcagagcca
      181 ccggataagc gccgtcgtga cctggacaat atcctgaaag caccactgga tgtgctgacg
      241 catgccggac ttctcataga cgacgagcag tttgatgaaa tcaatattgt gcgcggtcag
      301 ctcgttcctg gtgggcggct ggggataaaa atcacagaac tggagtgcgc atgaataacc
      361 actatttaca gtttgtgcgt gagcagctca ttatcgccac cgctgatttg agtggggcaa
      421 caaaaggtca gcttgaagcc tggcaggaga atgccatgtt cgatacaggg cgttacaggc
      481 gaaaaaaaat ccggtaccgc gatgaagtga ctggaaaaat gataacgcgg gataatccac
      541 caatcccggg aaagcaatcg ctggcgaagg tgacgtcaat tcctctggtc agtccggttg
      601 agttttcgac atcatcgtgg cggcgggctg ttctgtctct tgaagaacat cataaagcct
      661 ggttgttgtg gtgttacagc gggagtattt gttgggaata tcagatcgcg ataacacagt
      721 gggcgtggaa tgaatttaat actcaatccg gtaccagaaa aattgctggg aaaacgcagg
      781 aacgcctgaa aaaattaatc tggctggcgg cgcaggcagt aaaagcagaa ctttttggtg
      841 gggaaggtta tgaatatcag gagctggcat tactggcggg agtgacaact aaaaactggt
      901 ccaaaacatt tactcgtcac tgggttgcaa tgaaacacat ttttcaccga ctggatagtg
      961 aggctttatt atttgtaatg agaacacgtt caaaacaaaa ggcggcattt tcaaagcaaa
     1021 gtgttgcaaa agtagattga aaggcatata tttcatgcaa atctgatatt ttgccgattt
     1081 tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttgtt gtgccactta
     1141 tctcggatag acatggtgaa tgcgctggtg gaggagataa gggtgatttt tgtatgcttg
     1201 caacattgat ttcgtaacgt tattatcctg cgcccggccc tttagctcag tggtgagagc
     1261 gagcgactca taatcgccag gtcgctggtt caaatccagc aagggccacc aaccgccact
     1321 agccatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga gtccccggtg
     1381 gtggtccatt atcggtattc tgcgttgtta gctcagcagg acagagcaat tgccttctaa
     1441 gcaatcggtc actggttcga ctccagtaca acgcgctaca cttattttcc cggctcgctt
     1501 ttgcgggcct tttttgtatc tg
//
LOCUS       DAZFKM010000370         1511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFKM010000370 DAZFKM010000000
VERSION     DAZFKM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1158
                     /locus_tag="ACHKAY_005563"
     CDS             <1..1158
                     /locus_tag="ACHKAY_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5703990.1"
                     /translation="GPLAKLRLGFSLLTGSMNAVRVLPALWGMVTGSVSLLGGAIGAL
                     FSPVGLIVAALAGAAILIWKYWDPIRAFFAGVFSGIMERLAPLRETFERFGPVFDAIG
                     SGISQVFNWFKSLLSPMESSKETLDKCTSAGEIFGNVLGGALQLVLTPAKMLLDTLAW
                     ILEKLGVLPDEAERARKKIEDAQRAAILQDKVALLQGDLAKINPPKPVENGNGTGGDK
                     PKDNKPLTDSNTGTLRRLSKIADNTGKLVDETKKRIGPGDIVFKNLPRALAVRGEWQE
                     RKIAQVSKPAPAINITPVVPAPLPPALVPVVAASSRPVAEAIRSPVASVPATSRNRET
                     VASGFGGEIHVHLHNVVTQNPRELAKLVGEMVRAEMERRARAGRGSFYDKD"
     gene            1171..>1511
                     /locus_tag="ACHKAY_005564"
     CDS             1171..>1511
                     /locus_tag="ACHKAY_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703991.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKK"
BASE COUNT          340 a          366 c          450 g          355 t
ORIGIN      
        1 ggcccgctgg caaaactgcg tcttggtttt tccctcctga ccggatcaat gaatgctgtc
       61 agggttctgc cagcactatg gggaatggtg acgggttccg tttcgttact ggggggcgct
      121 atcggggcgc tgttcagtcc ggttggtctt atcgtggctg cgcttgccgg agctgccatt
      181 cttatctgga aatactggga cccaatcagg gcattttttg ccggggtgtt cagtgggatt
      241 atggagcggc tggccccgtt acgcgaaacc tttgaacggt ttggtcctgt ttttgacgca
      301 atcggaagcg ggataagcca ggtgtttaac tggtttaaat cgctgctgtc accgatggag
      361 tccagcaagg aaacgctgga taaatgtacc agtgctggcg agatattcgg taacgttctt
      421 ggcggtgcgt tacaacttgt tctgacacct gcaaaaatgt tgctggatac gctggcgtgg
      481 atacttgaaa agctcggtgt gcttccggat gaagcggaaa gggcgcgcaa gaaaatcgaa
      541 gacgcacagc gtgcggccat tcttcaggac aaggttgcct tgcttcaggg ggaccttgcg
      601 aaaatcaatc cgccgaagcc tgtggaaaat ggcaatggca ccggaggtga taaacccaaa
      661 gacaacaaac cgctcacaga cagcaatacc gggacgctac gcagactcag caaaattgct
      721 gataacacag gtaagctggt tgatgagacg aaaaaacgca ttggccccgg cgatattgtc
      781 tttaagaacc tgccccgcgc acttgctgtt cgtggggagt ggcaggagcg gaagattgcg
      841 caggtcagta agcctgcccc cgcaattaat atcacacctg tggttccggc tccgctgcct
      901 ccggcgctgg tccctgttgt tgcggccagc tcccgcccgg tggcagaggc catacgatcg
      961 ccagtggcat cagttcctgc aacttcccgt aaccgggaga ctgttgcctc cggatttggc
     1021 ggtgaaattc atgttcatct gcataacgtt gttacgcaga atccccgcga actggcgaaa
     1081 ctggtcggtg aaatggtcag ggcagaaatg gaacggcgcg cccgtgccgg gcgtggcagt
     1141 ttttacgata aagattgagg agtcatggcc atgatgatga tctatggcat gtttgttttt
     1201 gagctgcgca cgctgccgca tcagcagtta cagcaaaaca aaagctggcg gcatgtgaaa
     1261 aatgaacgcg ttaaccgttc agcaagctgg cagtatatcg gcgcaggtga tgatcgcatc
     1321 gttctttctg gtgtgcttta tcctgaaatt acaggtggcg aagtgtcgct gtcgctgctg
     1381 accacgcagg cgtatacagg acgaccctgg cctttgattg atggcgtcgg gcagatttac
     1441 ggcatgtatg tcctgaccgg aacgaatacg acccgttccg agtttgatcg ctacggtaag
     1501 gcgaaaaaga t
//
LOCUS       DAZFKM010000371         1511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFKM010000371 DAZFKM010000000
VERSION     DAZFKM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1158
                     /locus_tag="ACHKAY_005565"
     CDS             <1..1158
                     /locus_tag="ACHKAY_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5703992.1"
                     /translation="GPLAKLRLGFSLLTGSMNAVRVLPALWGMVTGSVSLLGGAIGAL
                     FSPVGLIVAALAGAAVLIWKYWDPIRAFFAGVFSGIMERLTPLRETFERFSPVFDAIG
                     SGISQVFNWFKSLLSPMESSKETLDKCTSAGEIFGNVLGGALQLVLTPAKMLLDTLAW
                     ILEKLGVLPDEAERARKKIEDAQRAAILQDKVALLQGDLAKINPPKPVENGNGTGGDN
                     PKDNKPLTDSNTGTLRRLSKIADNTGKLVEETKKRIGPGDIVFKNLPRALAVRGEWQE
                     RKIAQVSKPAPAINITPVVPAPLPPALVTVVAASSRPVAEAIRSPVASVPVTSRIREP
                     VASEFGGEIHVHLHNVVTQNPRELAKLVGEMVRAEMERRARAGRGSFYDKD"
     gene            1171..>1511
                     /locus_tag="ACHKAY_005566"
     CDS             1171..>1511
                     /locus_tag="ACHKAY_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5703993.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKK"
BASE COUNT          337 a          362 c          449 g          363 t
ORIGIN      
        1 ggcccgctgg caaaactgcg tcttggtttt tccctcctga ccggatcaat gaatgctgtc
       61 agggttctgc cagcactatg gggaatggtg acgggttccg tttctttgct gggaggcgct
      121 atcggggcgt tgttcagtcc ggttggtctt atcgtggctg cgcttgccgg agctgccgtt
      181 cttatctgga aatactggga tcccatcagg gcattttttg ccggggtgtt cagcgggatt
      241 atggaaaggc tgaccccgtt gcgcgaaacc tttgaacggt ttagtcctgt ttttgacgca
      301 atcgggagtg ggatcagcca ggtgtttaac tggtttaaat cgctgctgtc accgatggag
      361 tccagcaagg aaactctgga taaatgtacc agtgctggcg agatattcgg taacgttctt
      421 ggcggtgcgt tacaacttgt tctgacacct gcaaaaatgt tgctggatac gctggcgtgg
      481 atacttgaaa agcttggtgt gcttccggat gaagcggaaa gggcgcgcaa gaaaatcgaa
      541 gacgcacagc gtgcggccat tcttcaggac aaggttgcct tgcttcaggg ggacctggcg
      601 aaaatcaatc cgccgaagcc tgtggaaaat ggcaatggca ccggaggtga taaccccaaa
      661 gacaataaac cgctcacaga cagcaatacc ggtacgctgc gcagactcag caaaattgct
      721 gataacacag gtaagctggt tgaggagacg aaaaaacgca ttggccccgg cgatattgtc
      781 tttaaaaacc tgccccgggc acttgctgtt cgtggggagt ggcaggagcg gaagattgcg
      841 caggtcagta agcctgcccc cgcaattaat atcacacccg tggtcccggc tccgctgcct
      901 ccggcgctgg tcactgttgt tgcggccagc tcccgcccgg tggcggaggc tatacgatct
      961 ccagtggcat cagttcctgt aacttcccgt atccgggagc ctgttgcctc cgaatttggt
     1021 ggtgaaattc atgttcatct gcataacgtt gttacgcaga atccccgcga actggcgaaa
     1081 ctggtcggtg aaatggtcag ggcagaaatg gaacggcgcg cccgtgccgg gcgtggcagt
     1141 ttttacgata aagattgagg agtcatggcc atgatgatga tctacggcat gtttgttttt
     1201 gagctgcgca cgctgccgca tcagcagtta cagcaaaaca aaagctggcg gcatgtgaaa
     1261 aatgaacgcg ttaaccgttc agcaagctgg cagtatatcg gtgcaggtga tgatcgcatc
     1321 gttctttctg gtgtgcttta tcctgaaatt acaggtggcg aagtgtcgct gtcgctgctg
     1381 accacgcagg cgtatacagg acgaccctgg cctttgattg atggcgtcgg gcagatttac
     1441 ggcatgtatg tcctgaccgg aacgaatacg acccgttccg agtttgatcg ctacggtaag
     1501 gcgaaaaaga t
//
LOCUS       DAZFKM010000372         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFKM010000372 DAZFKM010000000
VERSION     DAZFKM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            832..1434
                     /gene="fimB"
                     /locus_tag="ACHKAY_005567"
     CDS             832..1434
                     /gene="fimB"
                     /locus_tag="ACHKAY_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO5703994.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          456 a          269 c          329 g          456 t
ORIGIN      
        1 aggaaagatt tgcaagataa ctcagtgggc gtggtgaaaa taagtgttta cccgccatca
       61 ggctgagcat aattctcatc atgaaatatg tttcctggtt tgtggcttgt agctggtcac
      121 tcctgaagtc tctctggaga ggcttgttga tgttggtgtt ttcaggatga tgtttcactt
      181 agcttgtttg ccgaatagcc cggcgaacag ctgtgattga ggaaggttta agtcgcagtg
      241 accaaagcca tatttaccaa cggatgcaga tgaaaaaatc atctcctgct ttcccccata
      301 tctccaggat aaaaaggaat gtaacaatct catgaactaa actgacgaac cagccaggaa
      361 taatcgctag ggacctaaga attagcctga taatagctac taagaaatta ctgcgttcca
      421 tgaaatagcc attttgtggc aaatggagtt gtctaataat gtcatatgtg agacggatag
      481 ttgaacgaat attaaatttt gctgaatttt ttatgttgat tttacttgtt acagaacata
      541 tcacatgata tatagataag attagttgca ttaatgatga gggttattat tagattcgta
      601 tccgattgat aaatatataa aggtacatag catgcaagag catagcgttt gtatggcaac
      661 gttattataa gtaactgttg ctactccatt taagttcact cagaagaact ggtccactta
      721 cgttagttat taagcaaacg ttcgctttta taaacataat caggataaaa atgttggatt
      781 attgctaacc cagcacagct agtgcgcgtc tgtaattata agggaaaaac gatgaagaat
      841 aaggctgata acaaaaaaag gaacttcctg acccatagtg aaatcgaatc actccttaaa
      901 gcagcaaata ccgggcctca tgcagcacgt aattattgtc tgactttgct ttgttttatt
      961 catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga tcttaaggca
     1021 aagtgtatat atatccatcg attaaaaaaa ggcttttcaa caacgcaccc gctattgaac
     1081 aaagaagttc aggctttaaa aaactggttg agtatccgta cttcgtaccc gcatgctgag
     1141 agcgaatggg tatttttatc gcgtaaggga aatccgcttt ctcggcaaca gttttaccat
     1201 attatctcga cttccggtgg taatgccggg ttgtcactgg agattcatcc gcacatgtta
     1261 cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact tatccaggat
     1321 tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa tgcagggcgt
     1381 ttttacggca tctgggatag agccagagga cgacagcgtc acgctgtttt atagagatag
     1441 ttgagatacc agggatggtg ttaaaaacta cattattctt acgatatatc aaaaatgatt
     1501 aaccatttat
//
LOCUS       DAZFKM010000373         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFKM010000373 DAZFKM010000000
VERSION     DAZFKM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..270)
                     /locus_tag="ACHKAY_005568"
     CDS             complement(<1..270)
                     /locus_tag="ACHKAY_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703995.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHKAY_005569"
     CDS             complement(365..571)
                     /locus_tag="ACHKAY_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5703996.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHKAY_005570"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHKAY_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          378 a          340 c          414 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tggtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZFKM010000374         1457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFKM010000374 DAZFKM010000000
VERSION     DAZFKM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1180)
                     /locus_tag="ACHKAY_005571"
     CDS             complement(<1..1180)
                     /locus_tag="ACHKAY_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5703997.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     Y"
     gene            complement(1126..1347)
                     /locus_tag="ACHKAY_005572"
     CDS             complement(1126..1347)
                     /locus_tag="ACHKAY_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5703998.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
BASE COUNT          284 a          479 c          359 g          335 t
ORIGIN      
        1 gatacagtgc atcaccgccg gccaggggcg gcaggttctc ccgtttacgg gcctcattgg
       61 gcgtgagaag cgtatttttc accgcatccc ccagcgtttt catgcgccgc tcactgtcca
      121 ttctcagcag cgtggtgaca tcaaattctg tactctcgtt ttcccccgtt tccagcgcct
      181 catccagtaa cagttcaatg gactcaatca gcgtctgcag gcactgggaa taatactgct
      241 gctccagcgc ctccacgttg tcactggaag gcggttgtcc cacgccaatc ttgtaggccg
      301 ggacacggaa caccgaacag acaatttcag cggtcatctt cagttgttcc accgtctgcg
      361 catccacagg tgaaaacgtc gtggggttgt attttgcccc gttgctcaga atggccgttt
      421 tccccgcatt ttcgcctgta tacccgctgt cccagttgct cttcagtttt ttcgcatttt
      481 cttccgtaat actgccgggg atctcaatca ccccggacgg cctgccgcca tttctgaaaa
      541 aagacgtcga attttcctga atatgatgcc cctgcgtggc cgccagcccg gcgacataca
      601 ccggcggcaa tcctataagc ggatgaaaaa aacagttaaa ccggtcgtgg atcacttccc
      661 gggcaggcac cgtcaccgcc tccgtgatcc cgcagttccg gtccggcgtg atgcggtaga
      721 acacctcgcc gtcatccgcc accagaggtt caacccggct ccagtccaga atacgcagtt
      781 ctttgatctg cccccgggca ttacggattt tcagcaccac cgtattgcca tgacgcagtt
      841 tggcgttcag ccacagttca aaaaactgga tgcggttctg ctgggcgttg ggacgacgac
      901 agaggcgggc aatatccccc cggcgcgttt ccctgcgtat cccatgcgca tccgtctgca
      961 taagacgcag ccgcattttg gcgatatcct gggatatcag cgaaatacat gcaaacaccg
     1021 catgaaagga gaggacggct tcaggatcgg ctttcacgcc ctgctgccag gcgccggaaa
     1081 agggctcagc caccgcctga aacaggctgg tccagcccgc ctctcttacg tcacgtcctg
     1141 atttctggtt ttttcgggtt cgccgtaaaa ggttccacat tcgccatgct ccgcatcacg
     1201 tttctttttc tgacctgccg gacgtcgcac cgtgatgtac tccgcctttc ccaggcgaac
     1261 cagcacctcc gcacacggct gtgccacatc acggatatcc ccggcccggg catcatgcgt
     1321 gccctgcaga tatcggatct ttgccataac ctgttacggg aggcgcacgc ctcccgtcct
     1381 ccttatcaga ctcagccgcc ggacgcactg ccgtagttca ctccggtgat caccgccacc
     1441 gccacaccgc cgtcatc
//
LOCUS       DAZFKM010000375         1457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFKM010000375 DAZFKM010000000
VERSION     DAZFKM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1271)
                     /locus_tag="ACHKAY_005573"
     CDS             complement(<1..1271)
                     /locus_tag="ACHKAY_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5703999.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGHHIQANSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGESVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLDALSRRDAREDPFASAGKTVSS"
     gene            complement(1217..1438)
                     /locus_tag="ACHKAY_005574"
     CDS             complement(1217..1438)
                     /locus_tag="ACHKAY_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704000.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
BASE COUNT          291 a          461 c          366 g          339 t
ORIGIN      
        1 tgtgatgaaa ctgttttccc ggctgacgcg aacggatcct cacgggcatc acgacgggac
       61 agcgcgtcca gactgtagtt ctgctgctga agatacagtg catcaccgcc ggcaaggggc
      121 ggcaggttct cacgtttacg ggcctcattg ggcgtgagaa gcgtattttt caccgattca
      181 cccagcgttt tcatgcgccg ttcgctgtcc attctcagca gcgtggtgac gtcaaactcc
      241 gtgctctcgt tttcccctgt ttccagcgcc tcatccagta acagctcaat ggactcaatc
      301 agcgtctgca gacactggga ataatactgc tgctccagcg cctccacgtt gtcactggaa
      361 ggcggctggc caacgccaat cttgtaggcc gggacacgga acaccgaaca gacaatttca
      421 gccgtcatct tcagttgttc caccgtctgc gcatccaccg gtgaaaacgt cgtggggtta
      481 tatttcgccc cgttgctcag gatcgccgtt ttccccgcat tttcgccggt atacccgctg
      541 tcccagttgc tcttcagttt tttcgcattt tcttccgtta tactgccggg gatctcaatc
      601 accccggacg gcctgccgcc atttctgaaa aaagacgttg aatttgcctg aatatgatgc
      661 ccctgcgtgg ccgccagccc ggcagcatac accggcggca gccccacaag cggatgaaaa
      721 aaacagttaa accggtcgtg gatcacttcc ctggcaggca ccgtcaccgc ctccgtgatc
      781 ccgcagttcc ggtccggcgt gatgcggtag aacacctcgc cgtcatccgc caccagaggt
      841 tcaacccggc tccagtccag aatacgcagt tctttgatct gcccccggga gttacggatt
      901 ttcagcacca ccgtattgcc gtgacgcagt ttggcgttca gccacagttc aaaaaactgg
      961 atacgattct gctgtgcatt gggacgacga cagagacggg caatatcccc ctgccgtttt
     1021 tcacggcgga tcccctgtgt atcggtctgc atcaggcgca gtcgcatttt ggcgatatcc
     1081 tgggatatca gcgaaatgca agaaaacacc gcatgaaagg agaggacact ttccggatcg
     1141 gctttcacgc cctgctgcca ggcgccggca aaaggctcag ccaccgcctg aaacaggctg
     1201 gtccagccca cctcttttac atcacgtcct gatttctggt tttttcgggt tcgccgcaaa
     1261 aggttccaca ttcgccatgc tccgcatcac gtttcttttt ctgacctgcc ggacgtcgca
     1321 ctgtgatgta ctccgccttc cccaggcgaa ccagcacctc cgcacacggc tgtgccacat
     1381 cacggatatc cccggcccgg gcatcatgcg tgccctgcag atatcggatc tttgccataa
     1441 cctgttacgg gaggcgc
//
LOCUS       DAZFKM010000376         1453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFKM010000376 DAZFKM010000000
VERSION     DAZFKM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            388..537
                     /locus_tag="ACHKAY_005575"
     CDS             388..537
                     /locus_tag="ACHKAY_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO5704001.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            1313..>1453
                     /locus_tag="ACHKAY_005576"
     CDS             1313..>1453
                     /locus_tag="ACHKAY_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5704002.1"
                     /translation="MAIPYEPYGDLTMTYKYNPFWQQRIRETVRHALDVHPRLTALRV
                     DLR"
BASE COUNT          403 a          323 c          369 g          358 t
ORIGIN      
        1 taagagagat caaacacatg agatcaaata catgagttca tatatgtgaa ataaacacgg
       61 tgagtgtgat cagtgggcat gatgagaacg cagtagcaac agactgcgct atccctgata
      121 gctggcgtat aacctgcgta tataaaacgt accagcaacg cacgtgaaca gagtgcatta
      181 agcatccgtc gttgacaccg acctggtgag tgacagtctg agagaccggt taagtggtgg
      241 tacgggagta atgagtttgg ggagggtacc agaatgtcag tctggtacgg tatgctgaca
      301 gtatcacgtt aattgcagca taaagtgatg agaggctaat ggaagttaaa accagagcag
      361 ttccgtcgtt gttcatcccg ggagacactg gaaaaagtga tttcccacac gcgttataag
      421 cttactccag cggagatgga agccttcaac tctgcggtcg atcaccgact ggcagaactg
      481 acaatgaaca agctttacga tcgtgttccc gcttcagtct ggaaatatgt catctgagcg
      541 ttcggtaatc acaactgcat cgcgctaggt gttatccgct ttgtgattct tattggctct
      601 tctcaggtta ttaaaagctg aatatgttcc tgttgtcggg aaccaggata ataccgggag
      661 cgttaagcgg tatagcgaca acaaagaatt cgaaatcaaa agggaatggt gccggaggcg
      721 tgggcggtaa taggctgatt ttcaggtatt accggcttac aacgtgtggt tgatattgag
      781 gggctgtgag tttcgcgtat ttgtcagaaa cccagatgag ttttcctgta tgggacgaaa
      841 taaggcagcg gcaatacctg gaaaatttaa tatggtatga aggagaacct gtcggttacg
      901 cttaatatac acggtacact gtcagctcag gggatacatg aaccgttatc tcaccagcta
      961 cacagtatac acacactgac gctgacagta gtcagcagga ccacaataat cagcgccagc
     1021 agtatggtaa cgatacggtc tgtattccgg tagtagccga aggggtcgac agaaccacaa
     1081 tacgggcaac acactgtatt attagtaatc gcgtttccac agtccctgca cttcttttgc
     1141 gtatcaccat aactccctcc tcacagcctt acccgtaacc ggttttttac attaaaaaac
     1201 aacgctgaaa attcaaccgt cagtccggag acgaccgttc gggttatcac agagtgcctg
     1261 agacagtgtc ctgccggagg tcacttcagg gactctgcgc attttttacg acgtggctat
     1321 tccgtatgaa ccatacggag atttaaccat gacctacaaa tacaacccct tctggcagca
     1381 acgtattcgt gagacggtgc ggcacgcact ggatgttcat ccccgtctga cggcattgcg
     1441 ggttgatctg cgt
//
LOCUS       DAZFKM010000377         1452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFKM010000377 DAZFKM010000000
VERSION     DAZFKM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..225)
                     /locus_tag="ACHKAY_005577"
     CDS             complement(<1..225)
                     /locus_tag="ACHKAY_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5704003.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPAE"
     gene            411..1379
                     /locus_tag="ACHKAY_005578"
     CDS             411..1379
                     /locus_tag="ACHKAY_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO5704004.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT          363 a          357 c          380 g          352 t
ORIGIN      
        1 ctctgccggg tcaagccagg tgcatcgcag caggattttc gcagcaacag tcatctggtc
       61 atgttcaaac cacggcggtt gtccgtcatt tccccgctta agtgttaacg gacaaccaca
      121 ggacacacag cacaaaacgt cctgtcggtt gtgcttcgcg gcttctttcg ccgtaacaaa
      181 atggcctttg ctgtcattcg ccatatagca gttcagaatt cgcatatctc ctccttgtat
      241 cttgacaatt ggttaaaata actgtgatta gatttggcaa ggtgtatgca gcccgatccc
      301 tcaaggggtt catagcccgt agctcagcga atggcgcata cactgagcca ctcatctgaa
      361 acccgaacag ttgtcagtac gccataacgc atttgcaagg atgggtaatc atgagtcagc
      421 caggttcact ttgtgtggat atcgatgttt cgaaagcgat actggatatt gccgccagca
      481 gcgctattga acaattttct gtcggcaatg acagtgatgg ctttgacgcc atcattgctg
      541 agttgagaat gtacgccatt gcgctggttt taatgaaagc cactggagga cttgaaaccg
      601 ccatagtctg tgcgcttcag gcagaaggtt ttgagcttgc cgtggttaac cccagacaag
      661 ctcgtgactt tgcccgcgcc atgggatacc tcgcgaaaac tgatagtatt gatgccagag
      721 tgctttccca gatggctgag gttctcaacc ggcatccgga gcgggaacgc tttatcagag
      781 cactgccgga tactgaatgt caggttctca ctgcaatggt agttcgtagg cggcagttga
      841 ttacgatgct ggttgccgag agaaaccggt tgcaccctgc tcatccattg aacaggaaaa
      901 gcattaatat catcatcaaa gcactggaag acgagcttgc gcgactagat gctgacatga
      961 acagccatat ccatagccac ttcaaagagc ttgctaagcg actgagcagt attaaaggtg
     1021 ttggcaccat gacggctgct gcgctgctgg cggaagtacc cgaacagggt aacctctcca
     1081 ggcgtgaaat cagtgtgctt gtcggtatgg ttccagtaaa ccgggactcg ggcacgatga
     1141 gagggcgacg aaccatcttt ggcggcaggg ctggtgtgcg aacagccctg tacatggcag
     1201 cgcttgtcgc aacccgcttt aacccggtaa taaaggtgtt ttacatgcga ctgcttgccg
     1261 caggaaaacc caaaaaagtg gcgctggtta cctgtatacg caagctgctg actatcctga
     1321 acgcaatgct cagaaagaac gaagaatgga atgaatcgta ccatcacgtt gctccataat
     1381 ttttacgttc aagacagttg ctgagctgtc cggggaagac tgaaacctcc ggtctggcag
     1441 tgtgtgcagt ac
//
LOCUS       DAZFKM010000378         1450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFKM010000378 DAZFKM010000000
VERSION     DAZFKM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1450
                     /locus_tag="ACHKAY_005579"
     CDS             <1..>1450
                     /locus_tag="ACHKAY_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3491 domain-containing protein"
                     /protein_id="HFO5704005.1"
                     /translation="LESDTLQKINTFSKNITIQTADGVMANWDDESSTLSVYSIDMIA
                     WRDKNKTEKEPLPLDVIQLTNWRIHNSCSLFYDKYQVDIEANKLTYTILFPDTELPVQ
                     LNYASVIYGNHGAKYTFLDSGSKAITIHITDESGDYDTFDFRNIVFEHHTNDMFISFD
                     NQGGFVISISNNATNELTDINVFRKNMTSFDGCRSLIYLPSGDVFYISDIYKMSRGRK
                     SVKLNVEKKPDINDIINVAILETSYLQIKKIPNNDDGSYVLCLDNPNLSSYTLHFNDL
                     SGYISSIWDNIRGSFTPFHNNTVNIAPNEKKFISLTEPDKLSFNIDVFREALEVKNSY
                     KLSKFTWETYGDIVVSPEDRISHLELDGFNYFSQPELDAQISDTFSYLYDNFQIADGD
                     VHIKLLHLDRETKQITPHRIILQKYFIDSFAKTSISDRENNIYPVICDSPDHFTNDIY
                     RHPFRIVLGNKTLYPSEELIKFIRTSKEYLSN"
BASE COUNT          526 a          224 c          227 g          473 t
ORIGIN      
        1 ttttagaaag tgacacttta caaaaaataa atacattttc aaaaaatata acaatacaga
       61 cagcggatgg agttatggca aactgggatg atgaatcaag tactctgtct gtttattcaa
      121 tagatatgat tgcctggagg gataaaaata aaactgaaaa agagccttta ccacttgatg
      181 tgattcagtt aactaattgg aggatacata actcatgctc tttattttat gataaatatc
      241 aagttgacat tgaagccaat aaattaacgt atacaatttt atttcctgat acggagttac
      301 ctgttcaact taattatgct agtgttattt atggtaatca tggggcaaaa tatacattcc
      361 tcgactcagg aagtaaagct ataaccatcc acattactga tgaaagtggc gattatgata
      421 cttttgactt ccgcaatatt gtttttgaac atcacaccaa tgacatgttt ataagctttg
      481 ataatcaagg aggatttgtt atatcaatat caaataatgc cacaaatgaa ctgacagata
      541 ttaatgtatt ccgaaaaaac atgacttctt ttgatggctg tcgttcattg atatatttgc
      601 ctagcggtga tgttttttat atttcagata tctataaaat gtccagagga agaaaatcgg
      661 ttaaattaaa tgtagaaaag aaaccggaca ttaacgacat catcaatgtg gctattttag
      721 aaacttctta tctgcaaata aaaaaaatcc caaataatga tgatggcagt tatgttttat
      781 gtctagacaa ccctaacctc tcatcttaca cattacactt taatgactta tctggatata
      841 tatcaagtat atgggataat attagaggaa gcttcactcc ttttcataat aatactgtga
      901 atatcgcccc taatgaaaaa aaatttattt ccctaacaga gccagataag ttgtcattca
      961 acattgatgt ttttagagaa gcattagagg taaaaaatag ttataagtta agtaaattta
     1021 catgggaaac gtatggtgat atcgtggtat cacctgaaga tagaatttct catctggaat
     1081 tagatggatt taattatttt tcacaacccg agttagatgc tcaaatatca gacacctttt
     1141 catatctata tgataatttt cagattgctg atggtgatgt tcatattaag cttcttcatc
     1201 tcgatagaga aacaaagcaa ataacgccac atcgtattat ccttcaaaaa tactttatag
     1261 actcatttgc taaaacaagt atctctgaca gagaaaacaa tatatatcct gttatttgtg
     1321 attcacctga tcacttcaca aatgatatat ataggcatcc atttaggata gttctcggga
     1381 ataaaacatt gtatccatca gaagaactta taaagtttat aagaacctca aaggaatatt
     1441 taagcaatat
//
LOCUS       DAZFKM010000379         1447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFKM010000379 DAZFKM010000000
VERSION     DAZFKM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..1304
                     /gene="escD"
                     /locus_tag="ACHKAY_005580"
     CDS             84..1304
                     /gene="escD"
                     /locus_tag="ACHKAY_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO5704006.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
BASE COUNT          464 a          283 c          271 g          429 t
ORIGIN      
        1 tcctttctac tctattctta gttgatgtaa gttcaccata ttttttctca ttgttcaacc
       61 accaggatga ataaaattta aaaatgttat cctcatataa aataaaactt ctcaatggtg
      121 cgatgcgtaa tcgggaatta caacttccga tgggaaacct aacgataggc acagaagata
      181 acgatattgt ttattttccc cttgaacaag gcctcaatca gtttttactt gatatacgtg
      241 aggaaggtgt tttcctttta tctccggttg aattttggat tgatggacag cccaccccct
      301 atgaagcaga tcagtcatta cctgtcggaa aggtcatcga tatagcggga tgttgcttca
      361 tcattggtga tattgaccac accttgccat tgtctgatgt gccagaaagg ttctctgcca
      421 aaaatcgacg taaaaagcgt cttatcctgg ctagcgtgat tggtgctgcg attgcgttgt
      481 ctggcgccat cggcagttac gttttgcttt ccccaaaagc tgaaccccca gcgtttacca
      541 gagctgatgt ataccagcag ctaaaagaaa acaaacttca tgcaatcact ctggtatggc
      601 atgggaaaaa tgtcgcgctt tatgggcgtt gcgaatcaac cactgattta actccatttt
      661 ttaattacct gaaagaaaaa aatatttttt attacaacaa tatcatttgc aataaccaaa
      721 tcatctccgc gattaatgat attttaacgg aatacggtta taaggatatc atcattacaa
      781 aaggtaataa gcctggattc ttcctgttat cgggatacat tcccccttca ccaaaatgga
      841 gcgaagttga aaatctgtta ctgaatacgc caggggttgc tggatgggaa atacataaca
      901 acagtaacaa taaaatcaat gaactggcat cagagttcaa aaagaataaa ttaataaact
      961 atgtcaatat tttcaaaaag aatgatgtca tcattgttgc gggggaggtt tctcaacaga
     1021 acgagagtaa gatccttgcc attataaatg cgatgaacaa aaatagtaac gtcaagatac
     1081 tatttcagaa cattcagccg tacatttcag cagatatttt ccccggaaaa atactcagaa
     1141 tcagcggcac gatgaagaat ccaacaattg ctcttgataa cggtacaagc ctgggaattg
     1201 gtagcattct gaaaggaggc tatgtcatag acgcgattga tcctaaagat ggtatcaaca
     1261 tctcccgccc agacgaatat atacatattc cgttgtctta ttaacttatt attatcttcc
     1321 ccctcttcgg agggtttttt attgtgcaga tgttgtagat atggtaaaca aggtctctat
     1381 caaacaagat acacgaaaaa cttattccga aatgagacat tgattttaac atgaaacgtt
     1441 tattaaa
//
LOCUS       DAZFKM010000380         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFKM010000380 DAZFKM010000000
VERSION     DAZFKM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..131)
                     /locus_tag="ACHKAY_005581"
     CDS             complement(<1..131)
                     /locus_tag="ACHKAY_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5704007.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     "
     gene            70..252
                     /locus_tag="ACHKAY_005582"
     CDS             70..252
                     /locus_tag="ACHKAY_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704008.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
     gene            911..1411
                     /locus_tag="ACHKAY_005583"
                     /pseudo
     CDS             911..1411
                     /locus_tag="ACHKAY_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          425 a          282 c          301 g          431 t
ORIGIN      
        1 gcggctgcgt tagtcagctt tttctcatag ccggatgccg agacaatcat cacatcctct
       61 gcagcagcta tgctgctggc tgaaaatccg agacagggaa tgactacgga agccagagtg
      121 gttattcgca tattactaat ctccgatcat gttaaccggc aagacaatac acaaatacaa
      181 acaataatga gaataattta tatttgcttt ttgcaaaaag taatgattat tgacaaaacc
      241 aaatataaat aacgactgcc tcggcatgcc agttaacagc tatccgtatg gcgtgttcgg
      301 ttttgcgaat aaaaaatggt tactgtagaa gcggatttcc accacatgtc aaccatgaat
      361 ggtcagtgat gcgagacgac tgggatgatt tggtcgatga cttcttccgg cgaatactga
      421 tagaaacatt gaaatattgt aagagaaagc gttcaaaatt ctgtggcacg tgaaaaattc
      481 tacagcgtca tcaccttatt cagtcgacct tcaaaataaa cgtttaacag agacaatcac
      541 catttggatt gtccgctgag gaggcgaaca atttcctaga cagctttatc atgtcagccc
      601 acattgttgg cgctggcaat actaaggaca aacgatgggg aagtagcttt ttagcgttat
      661 tttcgggagt aaaaaacgtg gtatctttcc gtatgtcagt tgtatcttgc tggtattcgt
      721 agtcgtgata aggcatacaa cgatatttat cttgacgtta gtgaagaata agttgttttg
      781 tgatctatgt taatctggga ttacaatccg ggcttgatac atcgcatcac gacttttatt
      841 ctcttcagaa tagaatatgt tactccccgc tttacaggct caggatttga attacaaatt
      901 atacttttaa gtgaacaata accaagggta tttcttattt cctgaaacca ggcttggccc
      961 atttcgggat gagtttgaaa aaactcttga cgcatacgat agtgatgaaa taaacgaaac
     1021 aaattacgtc aacaaactca ggcgtcttgc ctgacatgct cctgacatca ttgatattca
     1081 cgctcatctg gcatatgcat ttgcgctctt tatgctgcaa ttctggcgaa tgttcattta
     1141 cagcgtcatc aggatgccgt tatactcatt gataaaatac taacatataa ccctgagaat
     1201 aatcagggaa cacgctggtt actgggggcg gaattgttac gtgctggcaa gcatggcagg
     1261 gctctgtacg tattgacagg acatgtcaat gaatttcccc cctactggta cgagcttgct
     1321 cttttacatt ttatgaatga ggcatatatt aatgctgcaa cggcatttcg atatggattt
     1381 ttaacaagta tatattgttg aaatgctttg ttagaatctt tatccattcc ctagggctg
//
LOCUS       DAZFKM010000381         1430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFKM010000381 DAZFKM010000000
VERSION     DAZFKM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..411
                     /locus_tag="ACHKAY_005584"
     CDS             37..411
                     /locus_tag="ACHKAY_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO5704009.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            408..1229
                     /locus_tag="ACHKAY_005585"
     CDS             408..1229
                     /locus_tag="ACHKAY_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5704010.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          371 a          285 c          412 g          362 t
ORIGIN      
        1 tttgcaaccg gcgtgctcgg gtaaaagagg ttactgatgc gtatagagtt tgttttgctt
       61 tacccgccga cggtgaacac ctactggcga cgtcgtggca gcacatattt tgtatcaaaa
      121 gccggtgagc gttatcgccg ggctgtggcg cttattgttc gccagcagcg gctgaaatta
      181 agcctgtccg gaaggctggc gataaagatt attgccgagc caccggataa gcgccgccgt
      241 gacctggaca atattctgaa agcgccgctg gatgcgctga cgcatgcggg agtgttaatg
      301 gacgatgagc agtttgatga aatcaatatc gttcgtggtc agccagtatc tggtggacgt
      361 ctgggggtga agatttaccc cataatgctt gaagggctgg tcaaaaaatg aaactggaag
      421 atttaccgaa atactactcc ccaaaatccc ccggcctgac tgatgcatcg gcctcaacgt
      481 cgaaagatac gctgagtatc actgatgtga tggccgcgca gggcatgaca cagaattggg
      541 ctgagatggg gttttctgcg ttccttggga aaatgggcat tagtatgaat gacagagagc
      601 gggcaacaga attgctgaca gaatatgcac tcagtcggtg tgatcgcgtg gcggcgttaa
      661 gaaaactccc ggcagaaata aaaccggcag tgatgcgtat tatggcttcg tatgcgtttg
      721 aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc ctgttgtcac ggaaaaaaat
      781 ttattgaaag cgaggttttt acaaacaaga tccagtatcc ggatggtaag ccgccagtgt
      841 gggcaaagtg cacaaaaggc gtgtatccgt cttactggga ggaatggaaa aaagtcaggg
      901 aggtggtaaa agttgcctgt ccggagtgtg gagggaaggg ggaggtttcc accgcctgta
      961 aagattgtcg tgggcgcggt gttgccattc atcgtgaaga gtcggtaaaa cgtggtatgc
     1021 ctgttatcag agactgccag cgttgtggtg gtcgtggcta tgaaagatta ccttcaacgg
     1081 aggcatttaa tgccatatgt aatgtaaccg atgccatatc tcttgataca tggaaaaaaa
     1141 cagttaaacg tttttacgat acgctggtgg tgcagtttga tattgaagaa gcatgggcag
     1201 aacaacaact gaaaaaggtg accagatagc tttgttgatt tttcccgaat ctgtggtaaa
     1261 tttgccctaa cgatgggcgt tttatgcctg acgttagaag attttttaca cccgtcgcca
     1321 ggcgggtttt tttatgactg aaatcacgcc agtacagtaa acgcgctggt ggttgtgaat
     1381 accggtcttt cagcttgctg gctttttcga caagagttat tggtgtgtca
//
LOCUS       DAZFKM010000382         1421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFKM010000382 DAZFKM010000000
VERSION     DAZFKM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(28..207)
                     /locus_tag="ACHKAY_005586"
     CDS             complement(28..207)
                     /locus_tag="ACHKAY_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5704011.1"
                     /translation="MATGSTNNKSQQLNARFPHDVVADLEKNLDEGESKAQFIVTAVK
                     GEIKRRQRRKAKEQE"
     gene            398..598
                     /locus_tag="ACHKAY_005587"
     CDS             398..598
                     /locus_tag="ACHKAY_005587"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5704012.1"
                     /translation="MRNHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQGVGL"
     gene            595..825
                     /locus_tag="ACHKAY_005588"
     CDS             595..825
                     /locus_tag="ACHKAY_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704013.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHVQ"
     gene            815..1039
                     /locus_tag="ACHKAY_005589"
     CDS             815..1039
                     /locus_tag="ACHKAY_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704014.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDISVIADRAHKHAARESECIFH
                     YLTFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            1032..1397
                     /locus_tag="ACHKAY_005590"
     CDS             1032..1397
                     /locus_tag="ACHKAY_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704015.1"
                     /translation="MHNLSISDLNSIQFDEKFTGQLLVNVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPSTSHHDDDLHFTGCMVSHYENGVEVSCERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
BASE COUNT          384 a          339 c          357 g          341 t
ORIGIN      
        1 ttactcacac cacggcgctg gtgatggtta ctcctgctct ttggccttgc gacgctggcg
       61 gcgtttgatc tcacctttaa cggcagtaac tataaattgc gccttgcttt caccttcatc
      121 taaatttttt tctaagtctg caacaacatc atgcgggaat ctggcattta actgttgcga
      181 cttattattt gttgaacctg ttgccattac tgaatcctct ctaagttggt gcgattcagt
      241 atacacaaaa aaaatttcag agaaagactt gaagtgcgat tcacttactg ataggttaaa
      301 aacgaatggt gcgatgcacc aaacaacaac gccccgcagt ggtggcacac atgcagggcg
      361 tctaaccacc aacgatagcg aaagtatcga ggtagctatg agaaatcata ccatacaccc
      421 gcaagggcgg gactcgcaca acctgaataa atacatctgg cgttttatcg ccctgagcac
      481 ggcacaaccg cgcgtgatta ccattgaggc caccagcgaa caggaagcac gccagcaatc
      541 tccggctggc tgcgtgatgg tatttgctgc ccgtattcgt cagggggtag gcttatgagc
      601 caggaaatca cactacaaca ggcagcagaa cgcgcccacc aaatcgaagt tatttgcgca
      661 ctggcagagg attaccctgg catgatgacc gacagcgaat caggggcaat catcggctta
      721 cttaaacgcc ttagcggtga ggtctgcgta ttcctgagcg atgaacagga aagaagaacg
      781 cttatttcta acgaaaaaaa atgcggaggg gtacatgtgc aataaaacca caccggacgc
      841 agccgccgcc gcgctcacta cgctgatgca cgcgcttatt gatatttctg ttattgctga
      901 cagggcgcat aaacacgcag ccagagaatc agaatgcatc ttccattatt taacatttgt
      961 gcagctaaaa gccgatcagg cactggataa ggccggaaaa attatcatgg ctgatgtgca
     1021 ggaggtgcac catgcataac ctgtcaattt ctgaccttaa cagcattcag tttgacgaga
     1081 aatttaccgg gcagttgctg gtcaatgtgg agaacgggcg catagtgcgt aattatcacc
     1141 tgccggatgg tgcaattgcc ggaagcgttg aagcattgct ggaactggcg gaacgtgcgc
     1201 gactgattaa gccgtcaacg agccatcacg atgatgatct gcattttacc ggatgtatgg
     1261 tgagtcacta cgaaaacggc gtcgaggtat cctgcgaacg gctgcgtgat gattgctgtt
     1321 tcggcacact gccggaattt atcgagttgc tgaccagttg cggttatcag gtcattcagg
     1381 ggggtaaaca tgcgtgatga tcgttttaat tccctgaaac a
//
LOCUS       DAZFKM010000383         1419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFKM010000383 DAZFKM010000000
VERSION     DAZFKM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..340)
                     /locus_tag="ACHKAY_005591"
     CDS             complement(38..340)
                     /locus_tag="ACHKAY_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5704016.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            438..809
                     /locus_tag="ACHKAY_005592"
     CDS             438..809
                     /locus_tag="ACHKAY_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704017.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            830..1021
                     /locus_tag="ACHKAY_005593"
     CDS             830..1021
                     /locus_tag="ACHKAY_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO5704018.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            1023..1301
                     /locus_tag="ACHKAY_005594"
     CDS             1023..1301
                     /locus_tag="ACHKAY_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO5704019.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          433 a          315 c          307 g          364 t
ORIGIN      
        1 acattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaact ccatcagata
       61 agcatgcact acaatcacct tcagcatgaa ctacatgtgt ttgccccaaa cgaatgccgc
      121 tcgcatactc aggccaaata agctcccaat catgaggtcg tagctccgcc ctacttactt
      181 ggccttccgt cgcagattcg atcataaggg cgcgggttgg agatatagct gttcgtccag
      241 acgccatttg cgataagtaa gatggcgata caccaagtct ggccgcgaat ttcttagcat
      301 caccaaccct taatgattta ataaactctt ttaatgtcat accttcctcg gtttagtgtt
      361 tttttgcaag tttagtgttt aataaaccat taagtcaagt atttgcttgt ttagtgatta
      421 ctaaagataa ttaccacatg caaaaaaaag aaattcgccg tttacgtctc aaggagtggt
      481 ttaaagataa aactctgcca cccaaagaga agagctacct atctcaacta atgagtggga
      541 gagcctcgtt tggagaaaag gctgccagaa gaatagagca aacatacggg atgccggaag
      601 ggtatctgga tgcggaatac gcagaacaac cgggggtttc tccaccacat gcagggttaa
      661 cgtctaatca actggaatta ttgcagattt tttcagcctt ccctgaggat gagcaacgcc
      721 agataatcag cgagttaaag cagaaaaaag aatcaatgga agatctcata gcgagatgga
      781 ttgcggcgca aaaatgccgc cgcgcctgag ttataaaacc ggaggaaaca tgaatagagc
      841 cctttcacca atggtttctg aatttgaaac cattgaacaa gaaaacagtt acaacgaatg
      901 gctgcgtgcg aaagtagcaa cgagccttgc agatccgcgc ccagcaattc cccatgacga
      961 agttgagcgc agaatggcag aacgctttgc taagatgcgc aaggaacgga gcaagcagta
     1021 aaatgttacc cgtgttatgg cttgaaagcg cagataccga cctagatgat ataactagtt
     1081 atattgctcg tttcgacata gatgcggctg aacgcttatg gcagcgatta aggggttgtg
     1141 tgctgccgtt atccgaacat ccgtatttat acccaccaag cgacagagta cctggcttgc
     1201 gtgagattgt agcccaccct aactatataa ttctataccg cgtaacaaca tcaagcgttg
     1261 aagtagtaaa cgtgatccac gcaagacgcc agtttcccta actttcacta ccaatagaaa
     1321 cataacaacc gcaacgactt tatcaaaagc gttgtgtttg ttatgtcccg cggtttagtt
     1381 tttacttgac ttaagtttaa tgtttattaa actaaaaat
//
LOCUS       DAZFKM010000384         1401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFKM010000384 DAZFKM010000000
VERSION     DAZFKM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1347
                     /gene="rhsB"
                     /locus_tag="ACHKAY_005595"
     CDS             <1..1347
                     /gene="rhsB"
                     /locus_tag="ACHKAY_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO5704020.1"
                     /translation="THQYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWR
                     RERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGE
                     LAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCG
                     LTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEEN
                     PHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPI
                     SDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICED
                     KEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWD
                     EIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1319..>1401
                     /locus_tag="ACHKAY_005596"
     CDS             1319..>1401
                     /locus_tag="ACHKAY_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704021.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTY"
BASE COUNT          400 a          320 c          381 g          300 t
ORIGIN      
        1 acccaccagt accattacga cagtcagcac cggctggtgc actacacgcg gacacaatat
       61 gcagagccgc tggtcgaaag tcgctatctt tacgacccgc tgggccgcag ggtggcaaaa
      121 cgggtatggc gacgtgaacg ggacctgacg ggctggatgt cgctgtcacg gaaaccggaa
      181 gtgacctggt acggctggga cggcgaccgg ctgaccacga tacagaacga cagaacccgc
      241 atccagacga tttatcagcc ggggagcttc acgccactca tcagagttga aaccgccacc
      301 ggtgagctgg cgaaaacgca gcgccgcagc ctggcggata cccttcagca gtccggcggc
      361 gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc agatgctcga ccggctggaa
      421 agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc gctggctggc atcgtgcggc
      481 ctgactgtgg agcagatgca aaaccagatg gacccggtgt acacgccggc gcgaaaaatc
      541 cacctgtacc actgcgacca tcgcggcctg ccgctggccc ttatcagtaa ggaaggggca
      601 acagaatggt gcgcagaata cgatgagtgg ggcaacctgc tgaatgaaga gaacccgcat
      661 cagctgcagc agcttatccg cctgccgggg cagcagtatg atgaggagtc cggcctgtat
      721 tacaaccgcc accgctatta tgacccgctg caggggcgat atatcactca ggatccgatt
      781 ggactgaagg ggggatggaa cctgtatgga tatcaattga atccgatatc agacatcgac
      841 cccctgggtt tatctatgtg ggaggatgca aaatcggggg catgtactaa tggtctttgc
      901 ggcacactat ccgctatgat aggtccagat aaatttgatt ctatagatag caccgcatat
      961 gacgccttaa ataaaataaa tagccaatct atttgcgaag ataaagagtt cgctggttta
     1021 atatgtaagg ataatagtgg cagatatttc tcaacagcac ctaaccgagg agaaagaaaa
     1081 ggatcatatc cattcaatag cccttgccct aatggtactg agaaagtatc agcttatcat
     1141 actcatggtg cagatagtca tggagaatat tgggacgaaa tattttcagg taaagatgag
     1201 aaaatagtta aaagtaaaga taacaatatc aagtcatttt atttaggtac gcccagtggt
     1261 aattttaaag caatagataa ccacgggaag gaaataacaa acagaaaagg attacctaat
     1321 gtctgcagag ttcatggtaa tatgtaaaaa aatattgttt aggaactgtg tcattgtatc
     1381 tttgtttgtt tttacataca a
//
LOCUS       DAZFKM010000385         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFKM010000385 DAZFKM010000000
VERSION     DAZFKM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1160)
                     /locus_tag="ACHKAY_005597"
     CDS             complement(<1..1160)
                     /locus_tag="ACHKAY_005597"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5704022.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKYFDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIR"
     gene            complement(1224..>1398)
                     /locus_tag="ACHKAY_005598"
     CDS             complement(1224..>1398)
                     /locus_tag="ACHKAY_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704023.1"
                     /translation="AWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLN
                     PEPKKKAYEVFFI"
BASE COUNT          291 a          434 c          367 g          306 t
ORIGIN      
        1 cggatattga acggcacctg acgaagtgca gggatcccgc cctgaccaaa tcgcccgata
       61 atggtctgcg gacgcaggta atcaataaag tcctgcgcat attcctggta ttcagacagg
      121 ctgcctgccc actgcgggtc cgtggtggtc cctgccccca ccgccgattt caggacatga
      181 tgcagacggc tgtcatccgg atactgacga cgggccactt ccagggcttc agagcggaca
      241 cctttagccg cggccagtga tttggcaaag cgggcgaaac caatcccctt ctccagtttc
      301 tgctcaacac ggatcaccgg cgctgaagcc accgtggcca cattcccgtt accggcctgt
      361 ttcaccggct gtgccgtggc ggccttactg gtttccagtt cacgcagacg cttcaggtgc
      421 gcatccacct gacggatttc cgctgcggtg ttgtcgtagt gctcttcctc ttccacatcc
      481 agtgtgcggc cttcctctgc ggctttgttc atgatctcct caagggaggc tgccagcgcc
      541 gcacgcttgt tttcaaaact tttaatctgt tcaccagtat tcattgctga cttttcctta
      601 tgaaaagagg ttattgactg tgccgaagcg ccggcagaag atgcgatttt caccaccggt
      661 ttccggttgc cggacgcggc agaaaacggg cggtcgaaat atttaatggt ccggatggtg
      721 cattccgcat tcgcgggcac ggtgacggca gacacctcca tcagctccca gcgcagaaaa
      781 tgcagtccgc ctccgtccag ataagtgtat tcatggggcc ggaagcccac agaaagcccc
      841 ctgaccagcc cggtcttaat ggcagcccag gcctcatcca gccgggctgc catctgggag
      901 ggcatccccg gctccggctt caccagcatt gccgtgattt ccagcccttc cctgacccga
      961 cgcaccgtac actggcctac agggcgggaa tggtcatgct gccagagaaa cgggatcgta
     1021 ctgccaaact ccgccccctc cggctccagg atgtcaccat cccgatccgg agaaggcgtt
     1081 gacgcaatcc cggtgatcac ccgttcatcc tcactgaagg atttcaccgt cagcagggaa
     1141 caggcccgtt taagagtcac atcagcctcc tgaaaataaa aaaaccgccg cagcggttca
     1201 tgatggttac agggtgagca gggttatatg aaaaaaacct catacgcttt ctttttcggt
     1261 tccggattca gggacatcag ggacaccgca ttgaagagcg ccatcagcgg gtcaattttt
     1321 ccccgtccgc tggcctgttt ggtgataaga atggcgttac ctttaggctc cacccgggca
     1381 ttaccgacac accaggcc
//
LOCUS       DAZFKM010000386         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFKM010000386 DAZFKM010000000
VERSION     DAZFKM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..255)
                     /locus_tag="ACHKAY_005599"
     CDS             complement(34..255)
                     /locus_tag="ACHKAY_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5704024.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(324..800)
                     /gene="radC"
                     /locus_tag="ACHKAY_005600"
     CDS             complement(324..800)
                     /gene="radC"
                     /locus_tag="ACHKAY_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO5704025.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(816..1301)
                     /locus_tag="ACHKAY_005601"
     CDS             complement(816..1301)
                     /locus_tag="ACHKAY_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO5704026.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
BASE COUNT          327 a          364 c          400 g          307 t
ORIGIN      
        1 gccagtcgta ctcgttttcc gggtgacgga acatcagttc agggtgacgc tcatcagccg
       61 gacatacgga ccaaaactgt ccttacggcg ttctgcaaac acggccagca caccgggaat
      121 atcctgcact tcacgaccgg tatacgcttc agcactgccg tgccagcggt atttgccggt
      181 acagaacgga aaaagacggg atgtcggatg ttgttggtga atacgcatgg cttcaccacg
      241 ggtgatgatt ttcatagtgt gatacctctg aagacagaag ataaaagtga aaacaggtgt
      301 gatgtggttg tgacggtgac gggttaaagc agaccgtgtt ccgcaaagga gaaaacctgg
      361 ctgccaccga ctatcagatg gtccggcacc cggatatcaa ccagggccag cgcctgtacc
      421 agacgttccg tgataaggcg gtctgcctta ctgggggtga cttcaccgga cgggtgattg
      481 tgtgccagca ccacggctgc ggcattgtga tacagggcac gtttaatcac ttcccgggga
      541 tggacttccg tgcggttgat ggtgccggtg aagagggttt caccggcaat cagctgattc
      601 tggttattca gatacagcac ccggaattct tcacgctcca gtcccgccat gttcagaatc
      661 agccattccc gtgccgcacg ggtggaggtg aaggccacac cgggttcatg aagatggcgg
      721 tccagggttt tcagagcccg cagaatgaga ctgcgctcgc cgggcgtcat ttctccgggc
      781 agaaaggaaa gctgctgcat tgtccctgcc tccattcagt cgatgatgcg cataatggcg
      841 ctgcattccg gatgctgcag ggcgtaatcc cgcagccggt aataatggac cgtcatggca
      901 taacactccg tacgacaggc atgatgactg tacgtcatca gacaggcagc aatgccagcg
      961 gcttccgggc tcatttcagc gcggttgccg ttcatggcat tgaacagtac ccatgtttcg
     1021 tcatcatcgt catccggttc gggggccata aatgccccgc cgttgttcag agtgtacaga
     1081 ttccagatac caccgcagta gtcttcgcac agacggtcca tccagccgaa aacacggggc
     1141 tccagggtca cccactgtgg aatgaggcca aaatgctgcg gccagaagct gatgcgctgt
     1201 tcatcaggga ctatggtggc aaccaactga ggctggtcat tccctgatgc agcggttacg
     1261 gaaacagaag atgtggtgga attatgtgaa atggttttca tgaaatgact ccttaaagga
     1321 caaataaatg cgtggttttc agggtaatgg ctgaatcagc gcaggctctc cagcagggtt
     1381 tccgccatca cccacaat
//
LOCUS       DAZFKM010000387         1397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFKM010000387 DAZFKM010000000
VERSION     DAZFKM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..1396
                     /gene="nleA"
                     /locus_tag="ACHKAY_005602"
     CDS             74..1396
                     /gene="nleA"
                     /locus_tag="ACHKAY_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO5704027.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPL
                     KNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKT
                     ENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLD
                     NPNTKYAVLTNAAANALGAVGGFAVSRFTGTDPMLSPHIGAMVGQAAGHAIQYNTPGL
                     KPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGADI
                     RAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSNYVDTFDIIQETRV"
BASE COUNT          444 a          298 c          298 g          357 t
ORIGIN      
        1 tcggatatgt gacactaata atatcaatgg attgatatta ttaatggata taaacatgca
       61 ataaggattt atcatgaaca ttcaaccgat cgtaacatcc ggaatcacca cacaaaacaa
      121 tcgacatcat cacgcagaac aaacgtcccc tacacaaata ccgcaatccg aattacctaa
      181 tggatgcgaa acgggatttg ttgttcatat cccagaggat atgcagcgac atgcaccgga
      241 atgcggtgaa acaacagctc tactgagctt gataaaagat gaaggtctgc tctctgggct
      301 ggataaatat cttgcacctc atcttgaaga aggctctgca ggaaaaaaag cattggatat
      361 gtttggttta ttcaatgtct ctcagatggc attagaaata cccagcaccg ttccgggtat
      421 ctctggtaaa tatggtgtcc agctaaacat tgtaaaacca gatattcatc ctacatcagg
      481 taattatttt ttacagatat tccctttgca tgatgaaata ggtattaatt ttaaagacct
      541 tcctggtcca ttaaaaaatg cattaagcaa cagcaatata ccaaccactg tatcgactgc
      601 tgcatccact attgcatcag ccactacttc gacggtaacc accgcgtcaa aagacccaat
      661 accatggttt ggattaacag ctcaagtagt tcgtaatcat ggtgtggaac ttcctatagt
      721 caaaactgaa aatggatgga agcttgttgg agaaactcct cttactcctg atggccccaa
      781 agcaaattat actgaagagt gggtgatcag accgggagaa gcagatttta aatatggtgc
      841 atctccacta caggcaactc tagggctgga gtttggcgca catttcaagt gggatttaga
      901 taaccctaat actaaatatg ccgttcttac caatgctgcc gcaaatgcgc ttggtgctgt
      961 agggggattt gcagtatcca gatttactgg tacagatcca atgttaagtc ctcatatcgg
     1021 tgcaatggtt gggcaagcag cggggcatgc catacagtat aatacccccg gattaaagcc
     1081 agacactatt ttatggtggg caggtacgac tctggggctg gcagatttaa acaaagccga
     1141 gacaggtgaa ggcagattca ctgactatcc tcgtatatgg tggcacgcaa gagaaggtgc
     1201 catcttccct aataaagaag atattgcccg tgcaacaggg gctgatatac gagctatgga
     1261 agaaggtata cccgttggac agcgacatcc aaatccagag gatgtggtca tcgatatcga
     1321 aagcaatggc tcaccacatc ataacccatc aaattatgtt gatacctttg atataatcca
     1381 agaaacaaga gtctaag
//
LOCUS       DAZFKM010000388         1384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFKM010000388 DAZFKM010000000
VERSION     DAZFKM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1033
                     /locus_tag="ACHKAY_005603"
     CDS             <1..1033
                     /locus_tag="ACHKAY_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5704028.1"
                     /translation="SAKNHLEKNQGIINSKLTKFNNTIEEKIHSLLKKWADEIKVDRS
                     DYYAKTISVVDSIDNDSQIENVFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLD
                     SAFSLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEH
                     PGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITI
                     EFSEEFKQIAITDIPLRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPA
                     IDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANF
                     VIQFNGVEF"
     gene            1035..>1384
                     /locus_tag="ACHKAY_005604"
     CDS             1035..>1384
                     /locus_tag="ACHKAY_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO5704029.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDH"
BASE COUNT          476 a          211 c          250 g          447 t
ORIGIN      
        1 gtcagcgaaa aatcaccttg aaaaaaatca aggtattatt aattctaaac taactaagtt
       61 taataacacg atagaggaga agatacattc tcttttaaaa aaatgggccg atgaaataaa
      121 ggttgatcga agtgattatt atgctaaaac tatatcagtt gttgattcaa tagataatga
      181 ttcacaaatt gaaaatgtgt ttaatttgct cgatagttta tatgttgagt cagttgatac
      241 cctaactttc aaatatcaat caataataaa aggtctcgat agattatttg aaggtataaa
      301 cttagattca gcattctcat tatctgaaga agaacgctca tattttgaag aaaaagctaa
      361 aagtttaaac gcgcttgcac agttaggtat tagtgttgag ataatatctc atgaacttga
      421 agaaatggat tctatggtaa ccagaggact aaactctctt cctacttctg taaaagaaca
      481 ccctggtttt tcattggcgt taaatgctca cagatcgctt actcaacaaa tacgtttctt
      541 atcacctttg aaaatatcag gttatcaatc caggcagaga ataactggaa aaaatatcat
      601 ggattatgtc ctgaagttct ttggggagcg tttcgaacgg caacgaataa ctattgaatt
      661 tagtgaagag tttaagcaaa tcgcaataac agatatacca ttaaggatct atcctgtttt
      721 tactaatatt atcaacaatg caatgtattg ggtcagtctg tcaaataata ggctcataaa
      781 gattggtttt gtgaattctt tggttatcat agcaaattct ggtccggcaa ttgataccga
      841 tgatatcccg cgactatttg aactatttta tagcaaaaga gcaaatggac atggggtagg
      901 tctgtatcta tgtcgagaaa accttgctgt tgcacatcat aaaatatggt attcagaacc
      961 tgatgaaggc gataactatt taataaaaga tggcgctaat tttgtgatcc agttcaatgg
     1021 agtggagttc taatatgaca gtggcaaatt ataattctct tgtccagaaa actttctgcg
     1081 aaaatgcaat tcgttccgtt gtcatgattg atgacgattt tctgacgtat tctgaatcaa
     1141 tcagggcgtt gaataacgaa gttgatttag actacaacaa aattgactca tctaaacgag
     1201 ccgctactct tgagagcttt tttcaatcta aaaatatgat ttgtgatgtt gacaatggtt
     1261 ctgttaattt cgatgtggat cggattagaa aatcagatct tattattgta gattatcatc
     1321 ttgataataa tgcacctgat aaaacactta aactattaca agatttgaaa gactccgatc
     1381 attt
//
LOCUS       DAZFKM010000389         1382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFKM010000389 DAZFKM010000000
VERSION     DAZFKM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..107
                     /locus_tag="ACHKAY_005605"
     CDS             <1..107
                     /locus_tag="ACHKAY_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO5704030.1"
                     /translation="VKKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
     gene            116..1258
                     /locus_tag="ACHKAY_005606"
     CDS             116..1258
                     /locus_tag="ACHKAY_005606"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO5704031.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(1294..>1382)
                     /locus_tag="ACHKAY_005607"
     CDS             complement(1294..>1382)
                     /locus_tag="ACHKAY_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704032.1"
                     /translation="FVIRGNVEQIEPNLLGVYAGLLLAMKNK"
BASE COUNT          457 a          225 c          268 g          432 t
ORIGIN      
        1 ctgttaaaaa acatgagtac aaagaaaaat ataaacactt gctttaccag aaaaaatttc
       61 tcgatggtgt ttttacagca attaaatata atgttatgaa aggttaatat acgagatggt
      121 tgataaaata atatttacgg ttactcctat tttttcaatt cctcctcgcg gagcggctgc
      181 tgttgaaacg tggatgtatc aggttgctca gcgtaccaat tttccaaatc gtattgtttg
      241 tatcaaaaat ccaggatact caaattacac ctttgttaat gataattgta gtattcatag
      301 ggttggattc agtcgcatat ataagcggtt atttcaaaaa tggacaagac ttgatcctct
      361 tccatattca cagagaatat tgaatattgc acatgatttt cctataacga aagaaagtgt
      421 tattgtagtt cataatagca tgaagctata cactcaaatt cgtaagcgag ctcctcaggc
      481 aagagtggtt atacatatgc ataatgcatt tgaaccaaaa ttgcttgagc aaaatgttaa
      541 aatgatagtg cctagtttat acttgaaaaa gtattaccaa tcttatttag caaatgcaga
      601 tattgagatt gtaccaaatg gaattgattt agaaacctat caatctaatt ttcagccgat
      661 cactagaagc gaacttaata tctcccctga agagaaaatt attttttatg ctgggcgtat
      721 cgtccctgac aaaggcatat tgctgttgat gcaatcattc gaaaaattgg ccgctgctca
      781 caaaaattta aaattagttg tgattggtga ttatacagaa atgagtaaaa gtgacaaagg
      841 tgcatatcaa cgcaatgtca gggaaattgc aaaacgtttg aaggatcgtt gtattatgtt
      901 aggaagcata ccgcctgaaa agatgcattg ttattaccca ctggcagatt tagtcgtcat
      961 tccttcccag ttccaggaac cattttgcat ggtggcaatt gaagctatgg gtgctggtaa
     1021 gcctgtttta gtgagtacgc gtggggggat gacggaattt gtcaaagaaa atactacggg
     1081 attccattta aaagaaccta tgactgcaga ctccataagc agcgatattt taaaaacgtt
     1141 agcgaatcct gaattaacag cagttgcaaa gcaaggtcag gactttgtgt ttgaacacta
     1201 cagttgggat ggcgtaactc aaagattcga agaggttatt cataattggt ttgaataaat
     1261 aaaaaggccg cattatgcag ccttttttat tttttacttg tttttcatcg ctaataataa
     1321 gccggcgtaa acgcctaata aatttggttc aatttgttct acgtttccac ggataacaaa
     1381 at
//
LOCUS       DAZFKM010000390         1367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFKM010000390 DAZFKM010000000
VERSION     DAZFKM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..289
                     /locus_tag="ACHKAY_005608"
     CDS             <1..289
                     /locus_tag="ACHKAY_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFO5704033.1"
                     /translation="VQSIAEIRSAFIPLSIMSFAIFMGIYNFMFGSVGLSIRGYKKEF
                     SYIVAITGVSTIILSLCLSYFFAEIGAAIAYVFAEFILLILILRIYKVKRL"
     gene            355..>1367
                     /gene="wzy"
                     /locus_tag="ACHKAY_005609"
     CDS             355..>1367
                     /gene="wzy"
                     /locus_tag="ACHKAY_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen polymerase"
                     /protein_id="HFO5704034.1"
                     /translation="MYYIIFVMVLGLWIIAFNYARANKLSNLVIVLIITTLFIINRQN
                     QDYEAYVDIFNVNELYAEIGYRWLIYGVKYLGGTHEVIIGLLGLFLGTTFLRLIQYSK
                     YTAFGLLLYMLCPMPIDIVQIRNTFLFLFVINSLIELEKEHKFNSLCFVFMAPLFHSL
                     GIVYVLAWVIIQFRTWRGYNKLMVFGLVLSFIVVPLLIKMLILLFNTRTLHAYIADGI
                     KVHSLIIWAGPYLFDLFLLCYFRKKIVITDLHTKQWIDIIFSLMLFLSVFSPLLLYID
                     EINRIFRNALLLKYLVMMSIAQYLSRPTRYILYSYLLLFTFALSIYYTLQIDYDYIVF
                     GLP"
BASE COUNT          395 a          194 c          238 g          540 t
ORIGIN      
        1 ggttcaaagt atcgctgaaa ttagaagcgc gttcatccct ttatcaataa tgtcttttgc
       61 aatatttatg gggatataca attttatgtt tggttcggtt ggattgtcca taagagggta
      121 taaaaaagaa ttttcttata tagtggccat tacgggtgtt tcaactatta ttttatcatt
      181 atgcctgagt tatttctttg ctgaaatagg cgctgcaatt gcttatgtat ttgctgagtt
      241 tatcttactt attctcatac ttagaattta taaagtgaaa cgattataat tcccggtaag
      301 aactgatagt gcaaaatcat catggctata gactgaataa attgcgggga aagaatgtat
      361 tacatcatat ttgtgatggt tttaggttta tggattattg cattcaacta cgctagagca
      421 aacaagctaa gtaatttggt aattgtgctt attattacta ctttatttat tattaatagg
      481 cagaatcaag actatgaagc gtatgttgat atatttaatg tcaatgaact ttatgccgag
      541 attggttatc gctggttaat ttatggtgtt aagtatttag gcggtaccca tgaagtcata
      601 attggcttgc tgggtttatt ccttgggacc acattcctac gattaataca atacagtaag
      661 tatacagcat ttggtttgct gctttacatg ttgtgtccaa tgcctattga cattgttcaa
      721 atcaggaata catttctttt tttatttgtc ataaattctc ttatcgagtt agaaaaagag
      781 cataaattta actctctttg ttttgtcttt atggcacctc ttttccatag tttaggcatt
      841 gtttatgtat tggcgtgggt aataatccaa tttcgtacct ggaggggtta taataagtta
      901 atggtttttg gactggtgct gtcttttatt gtagtgccac tcttgattaa aatgttaatt
      961 ttattattta acactaggac gttacatgca tatatagctg atggcattaa ggttcattct
     1021 cttattatat gggcaggtcc ttatcttttt gatctttttc tgttatgtta cttcagaaaa
     1081 aaaatagtaa taactgattt acatactaaa caatggattg atataatatt ctcgctaatg
     1141 ttatttcttt ccgtattttc accactactt ttatatatcg acgaaataaa taggattttt
     1201 cgaaatgcgc tattactgaa gtatctggta atgatgtcta tcgcacagta tttaagtaga
     1261 ccgaccagat atatacttta ttcatattta ctgttattca cttttgcatt atcaatatat
     1321 tatacactcc aaatcgatta tgattacatt gtatttgggt taccata
//
LOCUS       DAZFKM010000391         1360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFKM010000391 DAZFKM010000000
VERSION     DAZFKM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..291)
                     /locus_tag="ACHKAY_005610"
     CDS             complement(<1..291)
                     /locus_tag="ACHKAY_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO5704035.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQM"
     gene            complement(430..663)
                     /locus_tag="ACHKAY_005611"
     CDS             complement(430..663)
                     /locus_tag="ACHKAY_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO5704036.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            777..>1360
                     /locus_tag="ACHKAY_005612"
     CDS             777..>1360
                     /locus_tag="ACHKAY_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5704037.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQ"
BASE COUNT          383 a          278 c          315 g          384 t
ORIGIN      
        1 catctggatt tgttcagaac gctcggtctt gcacaccggg cgttttttat tggtgagtcc
       61 atcaagcgca tacttaaaag ctctgctaat cggactgatg tctgatgcca ttccgaaagc
      121 acacaagacc gaagcaataa atctccagtc cgttctgctt atcttcgatt catgacagcc
      181 aatcatcttt gccagaccgc gctgggtaag cgttgacagg ttgataagta aatctgtttc
      241 tgcgcgatca acatcacgct gtgatagttt gctgtaactt gtttgttcca tttcttaaga
      301 tttccaatag tgaatagcta gttgaaaggt atgcgtggaa acgcatatgg ccttagttgg
      361 tcagatatct tggaactcgc ttttcagcga cgtaggacga atgtccgttg ttacaaagag
      421 cggggttact tatgctgctg atgctctacg cgatacgaac accagatttt cctttttcac
      481 aggtttataa cccgtgaaat tacgagtggc ttcttcgatt gcattcgctt tatctgggga
      541 agctcttcga aacccatata caatctgatc aagatagcca actgaagtat tcgctaatgc
      601 ggcgagtcgc ttccattcct cactagaagc ctcttttcgc cagcgtagta gttcattact
      661 cattagtgcc tccgtttatc acacagagcg actttaccat tttgataaat aaaacgcaat
      721 gaagatttat caaaatgcat atttatccat ttgctaaata gagggagaat cgtgggatgg
      781 aaaacaaaga tattcgcaaa tcgaatctgg cgtttttgct agatgagcat aaaaaaatcg
      841 cgggtaacac taatgcaagc tttgccgata agcttggagt tagcccttct caactcacgc
      901 aagtctccgg tgaaaaaagc actcgaaaca taggggataa actagcaaga aaattcgaag
      961 ccgcacttgg gttacctaat gggtggcttg atttggtaca tgatgtaaca ccaattgcat
     1021 catgctcaga ttctttaact tttgtcggtc aggtaagaaa agggttagtg cgcgtggttg
     1081 gtgaggcaat tcttggtgtt gatggtgcca tcgagatgac cgaagagcgc gatgggtggc
     1141 tcaatattta tagcgatgat ccagatgcct ttggtcttcg tgtgaaagga gacagcatgt
     1201 ggcccagaat aaaatcagga gaatatgtac tcattgagcc taacaccaaa gtattcccgg
     1261 gggatgaggt gtttgtcaga actgttgaag gacacaacat gatcaaagtt cttggctatg
     1321 acagagatgg agaataccaa tttacaagca tcaaccagga
//
LOCUS       DAZFKM010000392         1354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFKM010000392 DAZFKM010000000
VERSION     DAZFKM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..295)
                     /locus_tag="ACHKAY_005613"
     CDS             complement(167..295)
                     /locus_tag="ACHKAY_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704038.1"
                     /translation="MADLLLSILVPVDAGKYRKGGSVPSQNMAGGIVRRLVIVGMT"
     gene            351..665
                     /locus_tag="ACHKAY_005614"
     CDS             351..665
                     /locus_tag="ACHKAY_005614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704039.1"
                     /translation="MTDLSIDITQMNSSLFCHISQKQHIAKFITIINGKVQKIIPLNA
                     FNTASYVRKVQFLILVMENHDEQRFSIIEVISGKREGYEGRAQQNAMKEGVREFLVKT
                     ED"
     gene            746..937
                     /locus_tag="ACHKAY_005615"
     CDS             746..937
                     /locus_tag="ACHKAY_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5704040.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFPGKFKPDPREETE"
     gene            complement(990..1223)
                     /locus_tag="ACHKAY_005616"
     CDS             complement(990..1223)
                     /locus_tag="ACHKAY_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704041.1"
                     /translation="MARSDYDIINLSLKHELNEWLTERGYAGLADNRNRLAEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
BASE COUNT          416 a          322 c          273 g          343 t
ORIGIN      
        1 atcctctcca ctacgccgga tatccgcggc ctgaagagat aaaggcaata ataaaacaga
       61 catcagtgtc agtgacagca aggacggaca gcgccggaat aagctttgtt cctcagccct
      121 ctcgttcctg caacataatt ttccagtcaa acgaaaaacc ttttattcat gtcataccaa
      181 caatcaccag cctcctgacg atacctcccg ccatgttttg cgatggcacg ctaccgccct
      241 tccggtattt acccgcatca accggtacca gaatgctgag caataaatct gccaattttt
      301 agctgactta tttttgatca ttatagaata atgatctgaa ctacagcaat atgacagatt
      361 tatcgatcga tataacacaa atgaacagtt ctttattctg ccatatatcg caaaaacagc
      421 acattgcaaa atttataacc ataattaatg gaaaagtgca gaaaattatt ccactgaatg
      481 cttttaatac agccagttat gtcagaaaag ttcagttttt gattctggtc atggaaaatc
      541 acgatgagca acgattcagc atcattgaag tgatttctgg aaaacgggaa ggatatgaag
      601 gaagagcaca acaaaatgca atgaaagaag gagtgaggga atttttagta aaaacagaag
      661 attaacaaaa cacatcagga ggcagagact cctgattcag tactaccaca tgaaaattta
      721 gttaactgtt accgttctga aatctatgtc agaagatatc ccattaccga aaatcaatca
      781 gcgatacaga gacgatcatg gtgcgcttgt caccgtgacc agtgttgagg aaacccgggt
      841 ggtgtttatg cgggacggct atccgcatcc ctgcatgcga ccgatgtaca actttccagg
      901 aaaattcaaa cccgaccccc gggaagaaac agaataaaac gaaatccctg ccggcaatca
      961 cactgcctga tggcagcata atccacttat taattctcat ccccggagac aagcccggaa
     1021 aaccactccg ggtgttcact gtatgccgtg ttcagcgcat cccaggaaac gtttatataa
     1081 aagctgtcct gcaatttgcg ggtaaccacc tctgccaggc ggttccggtt atccgccagt
     1141 ccggcataac ctctctctgt cagccactca ttcagttcat gtttcagaga cagattgata
     1201 atatcataat cggaccttgc cattcccgtt tccttagcac atgttttcag gcacggggat
     1261 aaagtataaa gaatgtcgta ctgctgctgc cagagcaata ttccccctga tggttgtatc
     1321 agagatggta tgccggtact ttgcgggttg ttcc
//
LOCUS       DAZFKM010000393         1353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFKM010000393 DAZFKM010000000
VERSION     DAZFKM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            14..1338
                     /gene="nleA"
                     /locus_tag="ACHKAY_005617"
                     /pseudo
     CDS             14..1338
                     /gene="nleA"
                     /locus_tag="ACHKAY_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
BASE COUNT          434 a          282 c          281 g          356 t
ORIGIN      
        1 ataaggattt attatgaaca ttcaaccgac catacaatct ggaatcacct cacaaaacaa
       61 tcaacatcat caaacagaac aaataccctc tacacaaata ccgcaatccg aattacctct
      121 aggatgccaa gctggatttg ttgttaatat tccagatgat atacagcaac atgcaccgga
      181 atgcggtgaa acaacagctc tactgagctt gataaaagat aaaggtctgc tctcagggct
      241 agacgaatat atagctcctc accttgaaga aggatccata ggaaaaaaac attggatatg
      301 tttggtttat tcaatgttac ccaaatggca ttagagatac ctagttccgt ttcaggcatc
      361 tctggtaaat atggtgtcca gctaaacatt gtaaaaccag atattcatcc tacatcaggt
      421 aattattttt tacagatatt ccctctgcat gatgaaatag gttttaattt taaagacctt
      481 cctggcccgt taaaaaatgc attaagcaac agtaatatat caaccactgc agtgtcgact
      541 attgcatcga ctggaacatc agccactact tcgacggtaa ccaccgagcc aaaaggccca
      601 ataccatggt ttggattaac agctcaagtg gttcgtaatc atggtgtaga acttcctata
      661 gtcaaaactg aaaatggatg gaagcttgtt ggagaaacac cacttactcc tgatgggccg
      721 aaagcaaatt acacggagga gtgggttatc agaccgggag aagcagattt taaatatggt
      781 gcatctccat tacaggcaac tctagggctg gagtttggcg cacatttcaa gtgggattta
      841 gataacccta atactaaata tgccgttctt accaatgctg ccgcaaatgc gcttggtgct
      901 ttagggggat ttgcagtatc cagatttgct agtacagatc caatgttaag tcctcatatc
      961 ggtgcaatgg ttgggcaagc agcagggcat gccatacagt ataatacccc tggattaaag
     1021 ccagacacta ttttatggtg ggctggtgcg acactggggg ctaccgattt aaacaaggcc
     1081 gagtttgaag tagctagatt cactgactat cctcgtatat ggtggcacgc aagagaagga
     1141 gctattttcc ccaataaagc agatattgaa catgccacag gtgctgatat acgcgcaatg
     1201 gaagaaggta tccctgttgg acagcggcat ccaaatccag aggatgtggt aatcgatatc
     1261 gaaagcaatg gcttaccaca tcataatcca tcaaatcatg ttgatatctt tgatataatc
     1321 caagaaacaa gagtctaagc tttaacttgt tta
//
LOCUS       DAZFKM010000394         1346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFKM010000394 DAZFKM010000000
VERSION     DAZFKM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..63)
                     /locus_tag="ACHKAY_005618"
     CDS             complement(<1..63)
                     /locus_tag="ACHKAY_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704042.1"
                     /translation="MSALLSPLSLQAADVRRSGDE"
     gene            738..929
                     /locus_tag="ACHKAY_005619"
     CDS             738..929
                     /locus_tag="ACHKAY_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5704043.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(982..1215)
                     /locus_tag="ACHKAY_005620"
     CDS             complement(982..1215)
                     /locus_tag="ACHKAY_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704044.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
BASE COUNT          421 a          317 c          264 g          344 t
ORIGIN      
        1 ttcatctccg ctacgccgga catccgctgc ctgaagagat aaaggcgata ataaagcaga
       61 catcagtgtc agtgacagca aggacggaca gcgccggaat aagctttgtt cctcagtcct
      121 ctcgttcctg caacataatt ttccagtcaa acgaaaaacc ttttattcat gtcataccaa
      181 caatcaccag cctcctgacg atacctccca ccatgttttg cgatggcacg ctaccgccct
      241 tccggtattt acccgcatca accggtacca gaatgctgag taacaaacca gctagaattg
      301 acttaatttt gatcattata gaataatgat ctgaactaca gcaacatgac agatttatcg
      361 atcgatataa cacaaatgaa cagttcttta ttctgacaaa tatcgcaaaa acagcacatt
      421 acaaaattta taaccataat tactggaaaa gtgcagaaaa ttattccact gaatgctttt
      481 aatacagttg gttagcttaa aaaatagcaa tttttgattt cagtcattga aagttgcaat
      541 gtttgacgat tcaacagcat ttaagtgagt ttcaggatat gggaaggaca tgaaaggagg
      601 agcgcaataa aatacacagg aagaagggga aaatgtttcg taaaaacaga aaattaacag
      661 cgcacatcag gaaacgcagt ctcctgattc agtgctacca catgaaaatt tagttaactg
      721 ttaccgtact aaaatttatg tcagaagatg ttccattacc gaaagtcaat cagcgataca
      781 aagacgatca tggtgcgctt gtcaccctga ccagtgttga ggaaacccgg gtggtgttta
      841 tgcaggacgg ctatccgcat ccctgcatgc gaccgatgta caactttctg ggaaaattca
      901 aacccgaacc ccgggaagaa acagaataaa acgaaatccc tgccggcaat cacactacct
      961 gatagcagca taatccactt attaattctc atccccggag acaagcccag aaaaccactc
     1021 cgggtgttca ctgtatgccg tgttcagcgc atccctggaa acgtttatat aaaagctgtc
     1081 ctgcaatttg cgggtaacaa cttctgccag gcgattccgg ttatccacca gtccggcata
     1141 acctctctct gccagccact cattcagttc atgttccaga gacagattga taatatcata
     1201 atcggacctt gccattcccg tttccttagc acatgttttc aggcacgggg ataaagtata
     1261 aagaatgtca tactgctgct gccagagcaa tattccccct gatggttgta tcagagatgg
     1321 tatgccggta ctttgcgggt tgttcc
//
LOCUS       DAZFKM010000395         1329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFKM010000395 DAZFKM010000000
VERSION     DAZFKM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.399"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..911)
                     /locus_tag="ACHKAY_005621"
     CDS             complement(<1..911)
                     /locus_tag="ACHKAY_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO5704045.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADRTARA"
     gene            complement(892..>1329)
                     /locus_tag="ACHKAY_005622"
     CDS             complement(892..>1329)
                     /locus_tag="ACHKAY_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO5704046.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLD
                     MHFSGLTYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVR
                     FGGDGGRDILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
BASE COUNT          291 a          438 c          343 g          257 t
ORIGIN      
        1 gcccgtgcag tcctgtctgc ccaggtctcc agcgtgccca tgttctcttt caggcggcgg
       61 gtctgctcat caaagccttt cgtggcgata tcgttcgccg cctgtaaggc cccggcctca
      121 tcaccggaac gctgcagctg cgcaacatac gcaatctgct ctgccgtcac gttacggaac
      181 tggcgcgcca tcgcaatcag ccccgacgta gggtcggtgg tcagttttcc gaaagcctct
      241 gcaaccttgt ccacctccac accggatgca gaagcaaaac gcgcgacact ctggttgatg
      301 gcatcaaact gttcaccacc acgcacaccg gcattcacca gggctgccag tgactcactc
      361 gcctggttaa acgtcagccc tgcggcctgt ccggctctgg agagcgtcaa catgcgatcg
      421 gcagtcagtc cggactgatt accggaaaga accagggttt tattaaacgc tgaaagcgtg
      481 gaatccccct ggtaccaggc gtacgccagc gcacctgtcg ccaccgccag cgaggtgacc
      541 ccgaccatcg gcagggtgat cgcaccggca agccccctga acatggggat catcccgccg
      601 aaggagtcct tcacctgacc gccctgttgc agcaggatca gccagggatt ctgaccaccg
      661 gcaagctgcg tggcgatatc cgtaaactgt gcgggcaggg ttcgcatggc cgctttatac
      721 tgcccgacgg aaatcccggc tttttgtgca gccagcgcct ggcggctcag gccctgttca
      781 acagcactgg cggtttttct ggcgtcggta tccagacctg aaaaatgacg ccttacccgg
      841 ctcatctgct catcgaaacg gaccgcatcc agactaaggt caataacaag atcaccaacc
      901 ggctgggaca tatctcacac ctccggaaat ccccgctgaa gccatcatta atgcggcatc
      961 atccaccatg acatccgcca catccgcaga cgataaaata tcgcgccctc cgtccccacc
     1021 gaaccggacg cctccggcaa gtcctgccgc tttctgcatc agcattttgt cctcatccgg
     1081 cctctccacc tgctcttcct catgccgggg gacaagcaga ctgaaatcag agggatgcat
     1141 atccggatcg caaaaaaaca ggctgagtac agcgtacgtc agcccggaaa aatgcatatc
     1201 cagctgggta tcctgaaaat aatgcgtgcg gtaaaaacgg tgccagtcgg catattcggt
     1261 ggatgtcatc ccggcaagca tggcgcgcca gtcgggtctc cccatctcac gcgccagtct
     1321 gagggcaaa
//
LOCUS       DAZFKM010000396         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFKM010000396 DAZFKM010000000
VERSION     DAZFKM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..142)
                     /locus_tag="ACHKAY_005623"
     CDS             complement(<1..142)
                     /locus_tag="ACHKAY_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704047.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     HFS"
     gene            224..1325
                     /locus_tag="ACHKAY_005624"
                     /pseudo
     CDS             224..1325
                     /locus_tag="ACHKAY_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          486 a          182 c          315 g          342 t
ORIGIN      
        1 ttgaaaaatg aagtttttga ctggattggt aaatggtcat aatgaacaac accagaaaga
       61 gaaaaatata aaatacatac agaaagacat gagagcaagg gacggggcag ggcaagatca
      121 tgaaaattgt gatgtaaatc acgattttca tcttggcttt aacgcctaca ggtgatcaaa
      181 atttaacctt tggaataact aaaaagataa aaaaggacgc caggtgagta ttcaaagttt
      241 gcttgattat atttcagtga tccctgatat acgacaacaa ggaaaggtta aacataaatt
      301 atctgctatt ttgtttctca ccgtatgtgc agtaattgca ggtgccgatg agtggcagga
      361 aattgaagat tttggacatg aaagacttga atggctaaag aaatatggtg attttgataa
      421 tggcattccg gtcgatgaca ccattgcacg cgttgtgagt aacattgaca gtttggcctt
      481 tgaaaagatg tttattgaat ggatgcagga gtgccatgaa atcactgatg gtgaaattat
      541 agcaatagat ggaaagacca taagaggctc ctttgataag ggaaaaagaa aaggagcaat
      601 ccatatggtg agtgcattct cgaacgaaaa tggtgttgta ctggggcagg tgaaaacgga
      661 agccaaaagt aatgagatta cagccattcc agagttgctt aacctactgt atttaaagaa
      721 aaatttgata accattgatg ctatgggctg tcagaaagat atcgcttcga agatcaaaga
      781 taaaaaagca gattatcttc tggcagtaaa aggcaatcag gggaaattac atcatgcatt
      841 cgaggaaaaa tttcctgtaa atgtgttttc taattataaa ggcgattcgt ttagtacgca
      901 ggagataagt catggaagaa aagaaacacg tttgcatatt gtcagtaacg taacgcctga
      961 acttttgtga ttttgaattc gaatggaagg gattaaaaaa gctttgtgta gcattgtcat
     1021 tcaggcagaa gaaagaagat aaatcagcag aaggtgtaag catccgatat tatatttcat
     1081 caaaggatat ggatgctaaa gaatttgcac atgctatcag agcgcactgg ctgatcgagc
     1141 acagtcttca ttgggtgtta gatgtaaaaa tgaatgaaga tgccagccgg ataagaagag
     1201 gaaacgcagc ctaaataata tctggaataa agaagatggc actgaattta ttaagggatt
     1261 gcaaagacat taagggtgaa gaggaaaaga aagaaggctg cgttaaacac agggaaagat
     1321 catca
//
LOCUS       DAZFKM010000397         1324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFKM010000397 DAZFKM010000000
VERSION     DAZFKM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..321
                     /locus_tag="ACHKAY_005625"
                     /pseudo
     CDS             1..321
                     /locus_tag="ACHKAY_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    2..66
                     /locus_tag="ACHKAY_005625"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            844..>1324
                     /gene="ehxC"
                     /locus_tag="ACHKAY_005626"
                     /gene_synonym="hlyC"
     CDS             844..>1324
                     /gene="ehxC"
                     /locus_tag="ACHKAY_005626"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO5704048.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     "
BASE COUNT          383 a          217 c          312 g          412 t
ORIGIN      
        1 ctgctggata agctgcgtga gcagtacggg gtcggaccgg tatgcagtga actgcatatt
       61 gccccgtcaa cgtattacca ctgtcagcaa cagcgacatc atcctgataa acgcagtgcc
      121 cgtgcgcagc gcgatgactg gctgaagaga gagatacagc gcgtatacga tgaaaatcat
      181 caggtatacg gtgtgcgtaa agtctggcgc cagttgttac gcgaaggtat cagggtggcc
      241 agatgtacag tggcgcgcct catggcggtt atgggacttg ccggtgttct ccggggtaaa
      301 aaggtccgta ctaccgtcag ccgatttacg ggccgtaagt aacgaagttg gatgcaaatg
      361 tcagatcgtg tgcgcctgtt atggctcggc tggtaagaga gccttatagg cgcatttgaa
      421 aaaactccag ctataccgga agatatttat tattaacagg agaggtttgc ttttctgtgt
      481 aggtttagat tttgtgtttg cagggtaaac aaaactgatt aactcgtcat ttcggtaact
      541 gaaatagttt gctgtattct gtttacaatc tctttaataa ggcatttgtc acgtggctat
      601 tcatatgaaa atcatacgtt atcttacaaa tcaatcatct gagtgttata atataactta
      661 gctgtgatat gtgtaagaat gtttaggcaa tatattatta tcgctaatgt tgttttggtt
      721 gggagagatt ctccttgcgg tagctttttg tttttagatg cttcttgctt aaaaagatat
      781 aatttctgtt cttttatata ggattcttta catttactga gttttcttat ttatttaaac
      841 attatgaaat ctaatgcttt tgacgtcatg gggaaggttg catggctatg ggcctgttct
      901 cctctgcata aaaaatggcc attatctgtt tttgctatta atgtcatacc tgctattcag
      961 acaaatcaat ttgctctctt aataaaggat gagcttcctg tagctttttg tagctgggcc
     1021 agtttagatc tggaatgtga ggtaaaatat ataaatgatg taacatcttt gtatgcaaaa
     1081 gactggatgt cgggagaaag aaagtggttt attgactgga tagctccatt tggacacaac
     1141 atggagttat ataaatacat gaggaaaaag tatccttatg agttattcag agcaataagg
     1201 ttagatgaga gcagtaaaac aggaaaaata gctgaatttc atggaggggg gatagataaa
     1261 aaactggcat caaaaatatt caggcaatac catcatgaac ttatgtctga agtaaaaaac
     1321 agac
//
LOCUS       DAZFKM010000398         1322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFKM010000398 DAZFKM010000000
VERSION     DAZFKM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1322)
                     /locus_tag="ACHKAY_005627"
     CDS             complement(<1..>1322)
                     /locus_tag="ACHKAY_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO5704049.1"
                     /translation="LKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRM
                     ILKSRQIGATWYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEV
                     DVELKGGDKIILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMAT
                     LSGLRRTYFSTPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTW
                     RQIVTLEDVVNHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVD
                     GYDDWKDWKPFAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETR
                     RVQGLEFEEQARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSS
                     KRSLVLKMLQIMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDR"
BASE COUNT          300 a          391 c          326 g          305 t
ORIGIN      
        1 cggtccgttt cgtaagtgat aaagccgccc ggtgttttca ccttacgcac ggcgttaaag
       61 gccgcgacca gctcgcgttc ggcgcgatcg tattcccacc gcccggcacg cattatttgc
      121 agcattttca gtaccagcga ccgttttgat gacagcgtga aggtgtacgg aatagcagca
      181 gggaaaaacc gcttcactat ctgataaaca gcctccccgt tcccgcccgt cacatcaatg
      241 ccgatgtgtt ccacgttgta gcgatacgtg aactcttcaa tgactctggc ctgctcttca
      301 aactccagcc cctgaacgcg tcgagtctcc accgttcgaa aacggccacc aggaacagcc
      361 ggaggaacca caacggacac agcgccgctg tcgccgttgc cactactgcc gtttgcgtca
      421 tacccaatcc ataccggacg attccccatc ggtcggggag caaaaggttt ccagtctttc
      481 cagtcgtcgt atccgtcaac accgcagcca atcaggatat tcaggttaaa tgccgattcc
      541 ccttcgcgga caaactcaca catatagaga ttgaggaact cgtcttcggt gttttcatca
      601 cgaatttcgt cgatatcggt gtgtttccag ccgtgattaa ccacatcttc cagcgtgaca
      661 atttgccgcc acgtccggtc agggcagata agcccgttat gcagcgtttt ccagtccaca
      721 gaaaaacgct ggcgtttatg cgaggccttt ttctcgttcc agcggtcgcc gttccagtag
      781 gcgtatgcct cgtgcgtttc ggtggatggc gtggagaagt aggtgcgccg cagtccgctg
      841 agggttgcca tagcgccagc caccttgcgc agttcagcaa agcgactgac ccagaaaaat
      901 tcatcaaaat aaaaattgcc cgtataggac tgtgccgacg cagcagaagt gccgagaaaa
      961 tgcagctctg cgccgttgga gaggatgatt ttatcgcccc ctttcagctc cacatcaact
     1021 tcagccgcgg ccttctgaat aatgctttta aactggaacg cctgacgacg cgacgcagac
     1081 aaaaaaatct ggttacgctg gtaaggttgc gccacatcgt cacgcagcgc catcagcagc
     1141 gcttcctgtg caaaatacca ggtcgcccca atctgtcggg atttcaggat catcctgtta
     1201 cgtatcccgg cttccctgca aagggtcagg gagtcaaacc agccccgctg atgccactcc
     1261 agcctgctga tgattttttc ccgcagtgcg gcaatctgtt ccggcgtgaa atgatttttg
     1321 ag
//
LOCUS       DAZFKM010000399         1322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFKM010000399 DAZFKM010000000
VERSION     DAZFKM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1322)
                     /locus_tag="ACHKAY_005628"
     CDS             complement(<1..>1322)
                     /locus_tag="ACHKAY_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO5704050.1"
                     /translation="LKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRM
                     ILKSRQIGATWYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEV
                     DVELKGGDKIILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMAT
                     LSGLRRTYFSTPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTW
                     RQIVTLEDVVNHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVD
                     GYDDWKDWKPFAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETR
                     RVQGLEFEEQARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSS
                     KRSLVLKMLQIMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDR"
BASE COUNT          297 a          389 c          327 g          309 t
ORIGIN      
        1 cggtccgttt cgtaagtgat aaagccgccc ggtgttttca ccttacgcac ggcgttaaag
       61 gccgcgacca gctcgcgttc ggcgcgatcg tattcccacc gcccggcacg cattatttgc
      121 agcattttca gtaccagcga ccgttttgat gacagcgtga aggtgtacgg aatagcagca
      181 gggaaaaacc gtttcactat ctgataaaca gcctccccgt tcccgcccgt cacatcaatg
      241 ccgatgtgtt ccacgttgta gcgatacgtg aactcttcaa tgactctggc ctgttcttca
      301 aactccagcc cctgaacgcg tcgcgtctcc accgttcgaa aacggccacc aggaacagcc
      361 ggaggaacca ccacggacac agcgccgctg tcgccgttac cactgctgcc gtttgcgtca
      421 tacccaatcc ataccggacg attccccatc gggcggggag caaaaggttt ccagtctttc
      481 cagtcgtcgt atccgtcaac accgcagcca atcaggatat tcaggttaaa tgccgattcc
      541 ccttcgcgga caaactcaca catatagaga ttgaggaact cgtcttcggt gttttcatca
      601 cgaatttcgt cgatatcggt gtgtttccag ccgtgattga ccacatcttc cagcgtgaca
      661 atttgccgcc atgtccggtc agggcagata agcccgttat gcagcgtttt ccagtccaca
      721 gaaaaacgct ggcgtttatg cgtggccttt ttctcgttcc agcggtcgcc gttccagtag
      781 gcgtatgcct cgtgcgtttc ggtggatggc gtggagaagt aggtgcgccg cagtccgctg
      841 agggttgcca tagcgccagc caccttgcgc agttcagcaa agcgactgac ccagaaaaat
      901 tcatcaaaat aaaaattgcc cgtataggac tgtgccgatg cagcagaagt gccgagaaaa
      961 tgcagctctg cgccgttgga gaggatgatt ttatcgcccc ctttcagctc cacatcaact
     1021 tcagccgcag ccttctgaat aatgctttta aactggaacg cctgacgacg cgacgcagac
     1081 aaaaaaatct ggttacgctg gtaaggttgc gccacatcgt cacgcagcgc catcagcagt
     1141 gcttcctgtg caaaatacca ggtcgcccca atctgtcggg atttcaggat catcctgtta
     1201 cgtatcccgg cttccctgca aagggtcagg gagtcaaacc agccccgctg atgccactcc
     1261 agcctgctga tgattttttc ccgcagtgcg gcaatctgtt ccggcgtgaa atgattcttg
     1321 ag
//
LOCUS       DAZFKM010000400         1315 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFKM010000400 DAZFKM010000000
VERSION     DAZFKM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(474..1106)
                     /locus_tag="ACHKAY_005629"
     CDS             complement(474..1106)
                     /locus_tag="ACHKAY_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO5704051.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          354 a          272 c          268 g          421 t
ORIGIN      
        1 cgtttattta aggcgtaata tcttctataa ataacccgca aaatatcgtc gtagctgcac
       61 tcgatatttt taaaatcctg tatcaagtgc tgaaaatcat ttcatcgcca cttccaattc
      121 ctttctgcat ggtaagtagt gaggacgaaa agaagaatat gataaatatt gattcgtggt
      181 aatcagagag agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga
      241 tttacaagta agggtatgat tacgcttttt aaagcgtacc ataaaacata acataataaa
      301 tgatttgtga tgacacggga ttattgggat ttactttgag gaagataaaa tgcgtgttta
      361 tagaaccgcg agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa
      421 attgtcggct ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc
      481 acaaacagtg cttcgctgtc gacgatgatt ccatcttccg cacctttaag cgccaggttt
      541 gcctcgtagt attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc
      601 tgtttaagcg gcatcagata ttggttttcc attacgccaa caccggccag gcgaagttct
      661 tccattgcgc cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct
      721 tctttttcgg gagttgctac ataactctca gaaattccga cagatgcatt aatgacaata
      781 tattggtcat cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta
      841 ctgtcatctg acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttctggatcg
      901 ccatgaaaca gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca
      961 gagatacgct ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt
     1021 gccgctaccg gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc
     1081 atcgtagcca taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc
     1141 cgccatcata ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg
     1201 gttatcttac agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtaggaat
     1261 ctgcttcctg tcgcagagaa ctattcttat gtaccacaaa gagatgtcat ttatt
//
LOCUS       DAZFKM010000401         1311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFKM010000401 DAZFKM010000000
VERSION     DAZFKM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..922
                     /gene="espB"
                     /locus_tag="ACHKAY_005630"
     CDS             <1..922
                     /gene="espB"
                     /locus_tag="ACHKAY_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO5704052.1"
                     /translation="QVMTVNSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEIQK
                     LLGKMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLGIL
                     GSFAAINSATKGASDIAQKTVSTSSKAIDAASDTATKTLTKATESVADAVEDASSVMQ
                     QAMTTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVDKITGST
                     PFIAVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEVRRAQDD
                     IASRLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            929..>1311
                     /gene="cesD2"
                     /locus_tag="ACHKAY_005631"
     CDS             929..>1311
                     /gene="cesD2"
                     /locus_tag="ACHKAY_005631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO5704053.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGI"
BASE COUNT          368 a          274 c          319 g          350 t
ORIGIN      
        1 tcaagtaatg acggttaatt ctgtttcgga gaatactacc ggctctaatg caattaccgc
       61 atctgctatt aattcatctt tgcttaccga tggtaaggtc gatgtttcta aactgatgct
      121 ggaaattcaa aaactcctgg gcaagatggt gcgtatattg caggattacc aacagcaaca
      181 gttgtcgcag agctatcaga tccaactggc cgtttttgag agccagaata aagccattga
      241 tgaaaaaaag gccgctgcaa cagccgctct ggttggtggg gctatttcat cagtattggg
      301 gatcttaggc tcttttgcag caattaacag tgctacgaaa ggcgcgagtg atattgctca
      361 aaaaaccgtc tctacatctt ctaaggctat tgatgcggct tctgatactg cgactaaaac
      421 gttgactaag gcaacggaaa gcgttgctga tgctgttgaa gatgcatcca gcgtgatgca
      481 gcaagcgatg actacagcaa cgagagcggc cagccgtaca tccgacgttg ctgatgacat
      541 tgccgattct gctcagagag cttctcagct ggctgaaaac gctgcagatg ccgctcagaa
      601 ggcaagtcgg gcaagccgct ttatggctgc agtagataag attactggct ctacaccatt
      661 tattgccgtt accagtcttg ccgaaggcac gaagacattg ccaacaacgg tatctgaatc
      721 agtcaaatct aaccatgaga ttagcgaaca gcgttataag tctgtggaga acttccagca
      781 gggtaatttg gatctgtata agcaagaagt tcgcagagcg caggatgata tcgctagccg
      841 tctgcgtgat atgacaacag ccgctcgcga tctcactgat cttcagaatc gtatgggtca
      901 atcggttcgc ttagctgggt aattgatcat ggtcgatacg tttaatgatg aagtgcttaa
      961 tcactatctt gaacaaaaag ggtacacaat acagaaggag tttctttgtg gcagtgcctt
     1021 ttttatcgga tggcggattg agacctcttt tttctcatta gcgtacagac ttgatgaaca
     1081 agaactgatt ttgtgctctt ttgaagcacg taaccaaaca gggcttaacg gccctgtttt
     1141 atcactgact cgcttgctcg aagagttgta ccaccatttt tcgggtatta agaaaatcag
     1201 tgcgatgaaa tctaagattg gttcagattc agaacgtcaa aagcgcgaag agttgtttaa
     1261 ttacttcatc agaaagggcg ctgtgcagca agaaacagaa gacggaattt g
//
LOCUS       DAZFKM010000402         1301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFKM010000402 DAZFKM010000000
VERSION     DAZFKM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..730
                     /gene="ais"
                     /locus_tag="ACHKAY_005632"
     CDS             128..730
                     /gene="ais"
                     /locus_tag="ACHKAY_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO5704054.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKAY_005633"
     CDS             complement(769..1194)
                     /gene="nudI"
                     /locus_tag="ACHKAY_005633"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO5704055.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          363 a          287 c          271 g          380 t
ORIGIN      
        1 aaaattttga ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt
       61 atcgccagaa atgtcgctca attttacggt aattgtctgg ttgcgcttgt ctataggtgg
      121 agtttacgtg ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt
      181 actggcgctc gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt
      241 gccacgtatc gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt
      301 gtttcgccat gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg
      361 tattacggtt aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat
      421 ccctgatttc gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt
      481 ttccgcgggt aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta
      541 tagtgcaatt aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca
      601 taatcattgc ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta
      661 tctggatggt ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt
      721 taaccactaa attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc
      781 tttcaaacgt aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg
      841 ttttacccac gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac
      901 gcagtcaaaa atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt
      961 ggtgcgaata tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc
     1021 cagttcttcg cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc
     1081 gccacccgaa agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag
     1141 caaataagca ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcataggggt
     1201 attccttata aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga
     1261 cagtaatctc ttttgcaatg ataactgcac ttattattat t
//
LOCUS       DAZFKM010000403         1293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFKM010000403 DAZFKM010000000
VERSION     DAZFKM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..579
                     /gene="nleB"
                     /locus_tag="ACHKAY_005634"
     CDS             <1..579
                     /gene="nleB"
                     /locus_tag="ACHKAY_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO5704056.1"
                     /translation="NIHKQESYPASERDNYLLGLLREELKNIPEGKDSLIESYAEKRE
                     HTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCIYLDADMIITDKLGVLYAPDGI
                     AVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLGKGIKRHFNY
                     SSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            628..>1293
                     /gene="nleE"
                     /locus_tag="ACHKAY_005635"
     CDS             628..>1293
                     /gene="nleE"
                     /locus_tag="ACHKAY_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO5704057.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGEIFSYMPGEIDSYMKYINN
                     KLSK"
BASE COUNT          447 a          193 c          263 g          390 t
ORIGIN      
        1 aatatccata aacaagaatc ataccctgcc agtgagaggg ataattatct gttaggctta
       61 ttaagagaag agttaaaaaa tatcccagaa ggtaaggact ctttgattga gtcatatgca
      121 gaaaaaagag aacatacttg gtttgatttt ttcaggaatt tggccatgtt gaaggctgga
      181 actttgttta cagagacggg aaaaactgga tgccataaca tatcgccctg tagcggatgt
      241 atatatcttg atgccgacat gattattacc gataaattag gagtcctgta tgctcctgat
      301 ggtatcgctg tgcatgtaga ttgtaatgat gagataaaaa gtcttgaaaa tggtgcgata
      361 gttgtcaatc gtagtaatca tccagcatta cttgcaggcc tcgatattat gaagagtaaa
      421 gttgacgctc atccatatta tgatggtcta ggaaagggta tcaagcggca ttttaactat
      481 tcatcgttac acgattataa tgctttttgt gattttattg aatttaagca tgaaaatatt
      541 ataccgaata ccagtatgta taccagcagt tcatggtaag tcttccggtt gtggttgaaa
      601 aatatcgttc tttaggtata gattcagatg attaatcctg ttactaatac tcagggcgtg
      661 tcccctataa atactaaata tgctgaacat gtggtgaaaa atatttaccc ggaaattaaa
      721 catgattact ttaatgaatc acccaatata tatgataaga agtatatatc cggtataacc
      781 agaggagtag ctgaactaaa acaggaagaa tttgttaacg agaaagccag acggttttct
      841 tatatgaaga ctatgtattc tgtatgtcca gaagcgtttg aacctatttc cagaaatgaa
      901 gccagtacac cggaaggaag ctggctaaca gttatatccg gaaaacgccc aatggggcag
      961 ttttctgtag atagtttata caatcctgat ttacatgcat tatgtgagct tccggacatt
     1021 tgttgtaaga tcttccctaa agaaaataat gattttttat acatagttgt tgtgtacaga
     1081 aatgacagcc ctctaggaga acaacgggca aatagattta tagaattata taatataaaa
     1141 agagatatca tgcaggaatt aaattatgag ttaccagagt taaaggcagt aaaatctgaa
     1201 atgattatcg cacgtgaaat gggagaaatc tttagctaca tgcctgggga aatagacagt
     1261 tatatgaaat acataaataa taaactttct aaa
//
LOCUS       DAZFKM010000404         1289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFKM010000404 DAZFKM010000000
VERSION     DAZFKM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(879..>1289)
                     /locus_tag="ACHKAY_005636"
     CDS             complement(879..>1289)
                     /locus_tag="ACHKAY_005636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704058.1"
                     /translation="HLNTYIPSLMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQH
                     RFYLLNHYTESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQN
                     IGQNPFNIIIVICTKEHIERLELMYQGKSDGFFT"
BASE COUNT          373 a          257 c          221 g          438 t
ORIGIN      
        1 tcatttccta ggtaggtagg aactcatttc ctaagtaggt aggaaaccac ttcctaggtt
       61 acttcctaaa tagatcggaa atcatttcct aggtgttatt tattcttatc gggtatcagt
      121 tttgataggg gtatcagact tgataggggt aacagaattc tgtgggggta gttcactatg
      181 acctacccct agctcactgt gagctaccgt tacattcaat catcttccga tgtgtaatat
      241 cgttacattc aataattagt ctatgtaaca tatcgttact accaactttc ttacaaagag
      301 catttcagat agctgaatgt aacctctcgt tacatccatt tgtcacatag cactctgtgt
      361 aatgcctttc tatgtgtgtg ccataatccc ccctatattc cctgtgtaaa tcatagaata
      421 tccctaactt attcctgtgt aattccatac taaaaattca atgaaaaaag tagcttaaaa
      481 gttcaattct tactttcttc acttttacat cactttcgca aatccttgtg taatgcggct
      541 tacagagcac catcagaaat tgttggctaa aaattgttct gcttgtttct taatgagttt
      601 tcttgtttgt gcaaatttta aaatttattt tgtttttaca gccttatttc gtgtaatgcg
      661 tggcatagca agcccttttc ttgtgttgtc aagacatttg gcaacgttca aacatctgtt
      721 tagaaatgcc aaattgttga tctaacaaat aaacctgtat aaacagaaca ccatttaatc
      781 tattcccctg tgtaatccac tacatcacac gataaaaaca aaaaactttt aattatattt
      841 catagcacac aaaagtcaac agaagccctc tcgtgctcct acgtaaaaaa tccatcactt
      901 ttcccctgat acatcaactc aaggcgttct atgtgttcct tagtgcatat tacgattatt
      961 atgttaaatg gattttggcc tatattttga cctgcacccg cataaacact ggtttcatcg
     1021 cttacagccc ttgtgagacg tggtgctgtt tctagcatct gatccgacat aaaaccgatc
     1081 attctcgtgt ttagtgaaag tttagtgaga gacattacag aaaggctttc tgtgtaatga
     1141 tttaataggt aaaaacgatg ttgattacgc aagaaaattc cttgctgata tttggggatt
     1201 ctggattttc cagatcgggg ggcgctgaaa atcgcgttaa gcacttctcg caatctagcc
     1261 ataagggaag ggatataggt atttagatg
//
LOCUS       DAZFKM010000405         1287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFKM010000405 DAZFKM010000000
VERSION     DAZFKM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.409"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(101..691)
                     /gene="tccP2"
                     /locus_tag="ACHKAY_005637"
                     /pseudo
     CDS             complement(101..691)
                     /gene="tccP2"
                     /locus_tag="ACHKAY_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT          365 a          232 c          323 g          367 t
ORIGIN      
        1 ttagatgtaa tctgacagat acctgtataa ataaccggta actgtcaggt cagagctaat
       61 acaggtaatt atattataat cacgagcgct tagatgtatt aataccatgc tctgcaagat
      121 gctgcatcag acgctgagcc acatcaggca gaggtcttga ttgttcattt tgtaccggcg
      181 gcgttggcgc aggccagtta ggtgccggag gaatatattc agccatattc cgggctggtt
      241 gaatgccatg ctctgcaaga tgctgcatca gacgctgagc cacatcaggc agaggtcttg
      301 attgttcatt ttgtactggc ggtggtggcg caggccagtt aggtgccgga ggaatatgtt
      361 cagccatatt ccgggctggt tgaatgccat gctctgcaag atgctgcacc agacgctgag
      421 ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga gtataagatg
      481 tgggacgttg aagtcctgat tctgtataag aattttttac cttaaaaata gttgttgcac
      541 tggcaggact gttgctggga gaaaaaggtg agctggtttt taccgtgtta ggtgtgcttt
      601 tgtgctgcga gacagtgaag gtactgctgc gtatagcact tgatatactg cgaggaaggc
      661 tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagag ctttattgga
      721 tagtcttatt tatccgttta catgaaatct accttttgtg aaattctcgt caatatttta
      781 ctcaaaatga tcggctgtag gaataaacta tttgatatta ttacgttttt agggttgttt
      841 tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga ggaatagaag
      901 ctttgtatca gacgggcttc cctaaataga gaagttaaat aatcaggatg atgctaatga
      961 atatcaggga caaggtgagt gtcttcatgt tcagtgaaac agggtgtcac aaaaaagatt
     1021 ggggggggct agagccttct gcacgaaaat agtgatgcga cagatctgag atagctttgt
     1081 atccttctgc ctggttcagg cctggagcag tccatgtttt acaaaaatac tcggcagcgt
     1141 aacgtgttgc aagagcggca tcagacgagg aacctgaggt tccgctcatg accgataatc
     1201 tcatccgaat aaaaatatac caattggtat accaagttta taccaagagc caaaaaagca
     1261 aaggggctac catttggtaa ccccttg
//
LOCUS       DAZFKM010000406         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFKM010000406 DAZFKM010000000
VERSION     DAZFKM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1285
                     /gene="rhsB"
                     /locus_tag="ACHKAY_005638"
     CDS             <1..>1285
                     /gene="rhsB"
                     /locus_tag="ACHKAY_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO5704059.1"
                     /translation="RVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKR
                     VVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFY
                     YNHHSQLTSATGPDGLEIRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDAT
                     GSRKTMTWSRYAQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIA
                     VKDTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAG
                     RVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHY
                     DEADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTV
                     HHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAV"
BASE COUNT          321 a          380 c          395 g          189 t
ORIGIN      
        1 ggcgggtgac agaacagcta aacccggcag gcttaagcta cacgtatcag tatgagaaag
       61 accacatcac catcaccgac agcctggacc gccgtgaagt gctgcacacg cagggcgaag
      121 ccgggctgaa gcgggtggtg aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt
      181 ttgacgccgt gggcaggctc aggacacaga cggatgccgc aggccggaca acagaataca
      241 gcccggatgt ggtgacgggc ctcatcacgc gcatcaccac gccggatggc agggcatcgg
      301 cgttttacta taaccaccac agccagttaa cgtcagccac cgggcctgac gggctggaaa
      361 tacgccggga atatgatgaa ttgggccgtc tgattcagga aactgcccct gacggcgata
      421 tcacccgcta ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca
      481 ccggcagccg gaaaaccatg acgtggagcc gttacgctca gttgctgagc ttcaccgact
      541 gttccggtta tgtaacccgt tatgaccatg accgcttcgg gcagatgacg gcggtgcacc
      601 gcgaggaagg cctgagccag taccacgcat acgacagccg tggacagtta attgccgtga
      661 aagacacgca gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg
      721 tcattgcccc ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccgtcc
      781 gtaccacgca gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc
      841 gcctgaccag tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga
      901 tacaggaaac cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac
      961 ttatccgcag cgaggatgag gggctggtca cccactggca ctatgacgaa gcagaccgcc
     1021 tcacgcaccg caccgtgaag ggtgaaaccg cagagcggtg gcggtacgac gaacgcggct
     1081 ggctgacaga catcagccat atcagcgaag ggcaccgggt gacggtgcat tacgggtatg
     1141 atgagaaagg ccgcctgacc ggtgagcgtc agacggtgca tcacccgcag acggaagcac
     1201 tgctctggca gcatgagacc agacatgcgt acaacgcgca ggggctggcg aaccgctgta
     1261 taccggacag cctgcccgcc gtgga
//
LOCUS       DAZFKM010000407         1275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFKM010000407 DAZFKM010000000
VERSION     DAZFKM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..612
                     /locus_tag="ACHKAY_005639"
     CDS             <1..612
                     /locus_tag="ACHKAY_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5704060.1"
                     /translation="APTITSGGSPPVFSLTSDGKLTAKNADISGRVNANAGTLNNVTV
                     NENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQI
                     IIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVID
                     MPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            683..>1275
                     /locus_tag="ACHKAY_005640"
     CDS             683..>1275
                     /locus_tag="ACHKAY_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5704061.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVSYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGY"
BASE COUNT          323 a          290 c          360 g          302 t
ORIGIN      
        1 gcccccacca ttaccagcgg tggcagtcct ccggtatttt ccctgacatc agacggaaag
       61 ctgaccgcta aaaatgcgga tatcagtggc agagtgaatg cgaacgccgg gacgctcaac
      121 aatgtcacgg taaatgaaaa ctgtacgatt aagggcatgc tggaggcgac tcaggtcaga
      181 ggtgacttcg ttaaagctgt atccaaatca ttcccgaaac aggctggtac gtggggtaat
      241 acggaaacac caaacgggac ggttacagtc accatcagcg atgatcataa ctttgaccgt
      301 caaatcatta ttccgcccat tatctttaac ggaatagcgt atagcgatcc gggaagtggt
      361 aataacccgg gaggtacaag atacacgggt tatggttttg aagttcgcaa aaacggtgta
      421 ttaatcgcat ccagagaaac taaaggggcc attcccggta gttacagtgc agttattgat
      481 atgcctagtg gtggtggtag cgtcactctg gagtttaaga ttttccagaa aggcaatcag
      541 ggggcaggga atatcaccga ctgtacggtg attgtgacca aaaaagcggc ttccggtatc
      601 agtattcgtt gaaatattta taaccccaat aaagggcgtc aggaatgacg ccttttttat
      661 tgcagaaaag cgagaggtaa ttatgcgtaa actttatgca gccattttgt ccgcagctat
      721 ttgtctggcc gtatccggtg cgtctgcatg ggcatctgaa catcagtcca cgctgagcgc
      781 ggggtatctt catgcctcga cgaacgttcc cggcagcgat gatctgaacg ggattaacgt
      841 gaaataccgt tatgagttta cggacacact ggggctggtg acgtcattca gctatgcagg
      901 agacaagaat cgccagctta cccgttacag cgatatccgc tggcatgaag attccgtgcg
      961 taaccgctgg ttcagcgtga tggcggggcc gtctgtgcgc gtgaatgaat ggttcagcgc
     1021 gtatgcgatg gcgggtgtgt cttacagccg tgtgtcgact ttctccgggg attatctccg
     1081 cgtaactgac aacaagggga aaacgcacga cgtgctgacc ggaagtgatg acggtcgcca
     1141 cagcaacacg tctctggcgt ggggagctgg cgtgcagttt aacccgaccg aatccgtggc
     1201 cattgatatt gcttatgaag gctccggcag tggcgactgg cgcactgacg gtttcatcgt
     1261 gggtgtcggt tataa
//
LOCUS       DAZFKM010000408         1266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFKM010000408 DAZFKM010000000
VERSION     DAZFKM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..612
                     /locus_tag="ACHKAY_005641"
     CDS             <1..612
                     /locus_tag="ACHKAY_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO5704062.1"
                     /translation="MSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAIT
                     RSSGDRHQFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYM
                     EGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVT
                     GFKSEIDNQDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            655..915
                     /locus_tag="ACHKAY_005642"
     CDS             655..915
                     /locus_tag="ACHKAY_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO5704063.1"
                     /translation="MFRCPLCGASARIRTSRSENDSNTVRKKYYQCNNLECGISFSTL
                     EAFHKFTSKHAPGVHSSEGIPWHELPASHRGNNQMSLPLSQN"
     gene            1107..1247
                     /locus_tag="ACHKAY_005643"
     CDS             1107..1247
                     /locus_tag="ACHKAY_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO5704064.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT          357 a          308 c          343 g          258 t
ORIGIN      
        1 atgtccttcc ttacgcgcat ggcggaaatg ctgggcgcaa tcaccacggt aaaaagcggc
       61 aatctgttat tcatcatgcc cggtggtggc gtgaacgcac agggccagcc gttgccctcg
      121 ttcgccatta cacgcagcag cggcgatcgc catcagttcc gtattgctga ccgcgaggcg
      181 tatacggggg tacgcgccta ctggcttgat cttaattacg ggaaaaagaa aaaggtcagc
      241 gtaaaacgcc gtaaaccgcc aaaacccaaa aaggagaaaa gcagcagccg tgaaggtgat
      301 tatatggaag gtgcggaagg caatgtgttt gtgttacgca agacttatca gaacgagcag
      361 gcagcaagac gcgcagcggc ggcaaagtgg cagcagctac aacgcggagc cgcatcattt
      421 tccatcacgc tggcacgtgg acgtgcagaa ctctaccccg aaatgcatgg cacggtaaca
      481 ggatttaaaa gcgagattga taatcaggac tggattattg caaaagccga gcacaccatt
      541 gataacagcg gctttaccac gcagcttgag cttgaagcaa aaatcccgga atggatagca
      601 gaaacagagt gagcaactta gaatagcggc agcaccacgt taagggaggt cgctatgttc
      661 cgttgtccgc tttgtggcgc atctgcccgt atccgcacca gtcgttcgga aaatgattca
      721 aacaccgtac ggaaaaaata ttaccagtgt aacaacctgg aatgcggcat aagtttctca
      781 acactggaag ctttccataa attcacatcg aaacacgccc ccggcgttca ctcttcagaa
      841 ggtatcccgt ggcatgagtt gccagcttca cacaggggaa acaatcagat gagtttgcct
      901 ttatctcaga attaacaggc agaattgccg gagtaacaaa aaagcgatag attacgcgcg
      961 ggtgcctttc ggctgatggt cggagggaat acccgaaggc cggatgtgga aaggccccgg
     1021 aaaacatctc tgtttaaccg aggccctaac atatctacct taagcaagtg ataggttagc
     1081 gcctctccaa caaaggagca agcgctatgt cgcaaaaatc gcttacggcc atcacgttct
     1141 gcgtgacggt aatcctcata atctggatgc tgcacggttc gctgtgtgaa atacggatga
     1201 gcttctgggg agcggagttt gcggcgttct tacagtgtaa gcagtaagga aaccgcgacg
     1261 ggggag
//
LOCUS       DAZFKM010000409         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFKM010000409 DAZFKM010000000
VERSION     DAZFKM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..369
                     /locus_tag="ACHKAY_005644"
     CDS             13..369
                     /locus_tag="ACHKAY_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO5704065.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            353..>1260
                     /locus_tag="ACHKAY_005645"
     CDS             353..>1260
                     /locus_tag="ACHKAY_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5704066.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPS"
BASE COUNT          330 a          328 c          349 g          253 t
ORIGIN      
        1 cgaaaaataa gcatggcaag accaccgaaa gcccccgcct acctggatga catcgccgtg
       61 aagcagtggc gggaaaaatc gcggcagctt gcggaacggg gagacctgac ccccgccgac
      121 tggagcaatc tggaactgta ttgtgtcaac tactccattt accggaaagc cgtcgcagac
      181 cttgcggcgc gcgggttcag cattgttaac agtcagggcg gcgagagcag aaaccccgca
      241 ttaagcgcga aatcagacgc tgaaagggtg atgataaaaa tggcctcctt gctcggtttt
      301 gacccgataa gccgccgtaa aaacccaccg gaaacagaag aagaggacga gcttgaccgc
      361 ctggaataag tacgcagaag acgtaaaaac gggcaaaatt ccggcctgta aacggctgaa
      421 acaggccgtt aaacggtact tttcggacct taaaagcccc ctttacacgt tcgatcgtga
      481 ggttgtggag cggtttattg ccttttccag ggtgtgcccg cacgtaaaag gaccgatgcg
      541 tggcagaccc attgagctgg agccgtggca gcagttcgcc tttgcgtgca tcctcggctt
      601 taaggttaag gccaccggac ggcgcaaata cacgagcgcc tttatcgaag tgccgcgcaa
      661 aaatgcgaaa tccacgaccg ccgcgattct ggctaactgg tttctgatta tggagaacgg
      721 tcagcaggat atctacaccg cagcggtgag ccgtgaccag gcgcggatcg tctttgatga
      781 tgcgcgtcag atgtgccttt tatcccgacc gttacgcagg cgggtgaata ttcaggcgca
      841 taaggtgata cacccgaaaa gcaacagcct gttaaagccg ctggcagcaa aagcggcaac
      901 catcgaggga acaaacccaa gcctttccat cgtggacgaa taccacttgc accccgataa
      961 cggcgtttat tccgcgcttg agctggggat gggcgcacgt cccgaagggc tgttatttgc
     1021 cattaccaca tcaggcagca acgtcgtttc agcctgtaaa cagcactatg attattgctg
     1081 ccagatcctg gacggcgaag aggttaacga ttcaattttt gtactgattt acgagctgga
     1141 cgacgaaaac gaggttgatg atccggcgat gtggataaag gcgaacccta atattgatgt
     1201 ttccgtcgat cgtgaaaaac tggcctcaac catccagaaa gcgcggggta ttccgtcgca
//
LOCUS       DAZFKM010000410         1254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFKM010000410 DAZFKM010000000
VERSION     DAZFKM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1254
                     /locus_tag="ACHKAY_005646"
     CDS             <1..>1254
                     /locus_tag="ACHKAY_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704067.1"
                     /translation="VTKTLSGVLNGVAGNIDNVATAAGALVAVGGARYFGNMASGAVS
                     ATAGLVTAARNEVALAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLA
                     ATQERLNRNIAARTAAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQN
                     QEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSL
                     QEKIAGYQYVLANPGWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKV
                     QSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQG
                     LVNVPLRLPQATLDDKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQ
                     EARQRYISNALEAWRNNEANKPKSRG"
BASE COUNT          301 a          303 c          422 g          228 t
ORIGIN      
        1 gtgacaaaaa cgctctccgg cgtgctgaac ggtgttgccg gtaatattga taatgtggca
       61 acagccgcgg gggcgctggt tgccgtcggg ggtgcccggt actttggcaa tatggcctcc
      121 ggagcggtgt ctgccacggc aggacttgtg acggcagcac gtaatgaagt ggcactggcg
      181 gaagcacagc tcagggggac gcagattgcc acggcgcggg caagggcagc cgtgtaccgt
      241 gcacagcagg ctgtggcggc agcccgcggg acggagatgc agattgctgc agaagcccgt
      301 ctggcggcca cacaggaacg cctgaacaga aatattgctg ccagaaccgc agcccagaat
      361 gagctgaaca gtacaacggc ggtgggctca cgtctgatga ctggtgcgtt gggactggtt
      421 ggtggcgtac ccggactggt gatgctgggg gcagcagcat ggtatacgct gtaccagaat
      481 caggagcagg ccagggagtc agcgcgccag tatgcactga cgatagatga aatcgcgcat
      541 aaaacgccgt caatgtcttt gcctgaagcc tcagataatg aaggacgaac acgggcggcg
      601 ctggcagagc agaaccggct gattgatgaa caggccagcc gggtgaaatc cctgcaggaa
      661 aaaatcgctg gatatcagta tgttctggct aaccctggct ggacaaccgg tgacggattc
      721 atgataaacc atctgacatc ggtgaagacc gtgacggaag ggctttctca ggcaacagag
      781 cagcttgccg ttgagcagtc ccgtctggca cagatgcagg aaaaagtgca gtccattcag
      841 gatgtgcttg ccgggctgga agaccgtcgt gtggcgttaa ttcgtcagca ggcggcagag
      901 cagaataagg tgtaccagtc catgctggtt atgaacggtc agcatacgga attcaaccgt
      961 ctgctggggc tggggaatga actgcttcag cagcgtcagg gactggtgaa tgtaccgtta
     1021 cggctgccgc aggccaccct ggatgataaa cagcagagcg ccctgacaaa aacagaacgt
     1081 gagctggccc tgtcccgact gaagggggag gaaaaagagc gtgcccggct ggggtatgcg
     1141 gcggatgacc ttggttttgt gggggattcg tatcaggagg cgagacagcg ttatatcagt
     1201 aatgctctgg aagcctggcg taataacgag gcgaacaaac ctaaatcccg gggt
//
LOCUS       DAZFKM010000411         1249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFKM010000411 DAZFKM010000000
VERSION     DAZFKM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1243
                     /locus_tag="ACHKAY_005647"
     CDS             <1..1243
                     /locus_tag="ACHKAY_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO5704068.1"
                     /translation="KPFPESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRS
                     AFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQS
                     EMYGRQGVELSRSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNK
                     KTKTGRLWTYVRDDRNAGSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFN
                     ELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQR
                     LAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEAD
                     NNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVI
                     ADWPINRVGELLPWRVALPTE"
BASE COUNT          274 a          356 c          392 g          227 t
ORIGIN      
        1 caaaccgttc cccgaatcac tcccccgcga tgaaaaacgg ctgctgccgg cagcatcatg
       61 ctgcccggaa tgtggaggct cactgagcta tctgggtgag gatgccgccg aacagctgga
      121 gttgatgcgc agcgccttcc gggttatccg gactgtacgt gaaaagcatg cctgtactca
      181 gtgcgatgcc atcgtgcagg cccccgcgcc ttcacggccc atcgagcggg gtatcgcagg
      241 accggggctg ctggcccgcg tgctgatctc aaagtatgca gagcacaccc cgctgtaccg
      301 ccagtctgaa atgtacggcc gccagggcgt ggagctgagt cgttcactgc tgtcgggctg
      361 ggtggatgca tgctgccggc tactgtcacc gctggaagaa gcgcttcagg actatgtgct
      421 gactgacggt aagctccatg ctgatgacac gcctgtcccg gtgctgttgc caggcaataa
      481 gaaaacgaag accgggcggt tatggaccta cgttcgtgac gaccgtaacg ccgggtcaac
      541 gctggcgccg gcggtgttgt tcgcttacag cccggacaga aaaggcatcc atccgcagac
      601 ccatcttgcg gggttcagtg gtgtactgca ggcggatgca tacgccgggt tcaacgagct
      661 gtaccgggat ggccggataa cggaagccgc ctgttgggct cacgcccgcc gtaaaatcca
      721 cgatgtgcac gttcgcaccc cgtcagccct gacggaggaa gcgctgaaac ggatcggcga
      781 actgtacgcc atcgaggcag agataagggg aatgacggcg gagcagcgcc ttgccgaacg
      841 tcagttgaaa acgaaaccgc tgctgaaatc cctggaaagc tggctgcgtg aaaagatgaa
      901 aaccctgtcg cgacactcag aactggcgaa agcgttcgca tacgccctga accagtggcc
      961 ggcgctgacg tactatgcag atgatggctg ggctgaggcg gacaataaca tcgctgaaaa
     1021 tgcgttgcgg atggtcagtc tgggccgcaa aaactacctg ttcttcggtt cggatcatgg
     1081 aggagagcgg ggagcgctgc tgtacagcct gatcgggacg tgcaaactga acggagtgga
     1141 gccagaaagc tacctccgct atgtccttga cgtcatagcc gactggccga taaaccgggt
     1201 cggcgaactg ctcccctggc gcgtagcact gccgactgaa taacacatc
//
LOCUS       DAZFKM010000412         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFKM010000412 DAZFKM010000000
VERSION     DAZFKM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..1202
                     /locus_tag="ACHKAY_005648"
     CDS             57..1202
                     /locus_tag="ACHKAY_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO5704069.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          412 a          262 c          234 g          338 t
ORIGIN      
        1 gctatatgat atctgttgtc gaatcattca taataaatga gacaggggaa tctctcatga
       61 taaacggact taataataat tccgcatctt tagttttaga tgctgcaatt agaattaatt
      121 ctgattttaa aaagcagtgg aatgatatgt catgcgctga aaaattacta aaagttctta
      181 gttttggttt atggaatcca acgtatactc gtagtgaaag gcaaacattt caagagttgt
      241 taaccgtttt agaacctgta tctcccgcgc ctaatgaatt gggcagaata tatgctaatt
      301 ttgcagatgg ctcatcctta agaatttccg tcactaacag cgaacttgtt gaagccgaga
      361 ttcgcacacc agataatgaa aagattctta tgctcctgga gtcaaacgaa caaaataggt
      421 tattacaatc tttacccatc aatctccaca tgccatacat tcaggttcat cgtgccttat
      481 ctaagatgga cctgactgat cataagtcaa tgcacaatct acttagtttt acgtcaaaac
      541 tatcagcaac cttgattcct cataatactc aaacagatcc gctttccggg cctacaccat
      601 tcagctctat gtttatggat acatttcgag gactcggcaa tgcaaagctt tcactcaatg
      661 gtgttgatat acctgtagat gcacaaaaat tgcttcgcga tgcactagga cttaaagaca
      721 cacattcatc acttgcccgg aatgttataa ataatggtat ttctcgtcat catgcaaagc
      781 aaatagcaag ggaaagcagc ggcagcgata aacagaaagc tgaagttgtg gagtttttat
      841 gccaccccga agcggcaaca gccatatgct cggctttcta tcaatctttc aatgtgccag
      901 ccttaatgtt gacacataca aggatctctc aagccagaga atacaatgtg gaaagatcgt
      961 tagatgtacc taacgcgtgc attaacataa gtatctctca atcaccagat ggaagcattc
     1021 acgttgccag ccataccggg attctgataa tggcgccaga agaccgcccc aacgagttag
     1081 gaatgttgac gaacaggacc tcctatgaag tgccacaagg tgtgaaatgt gaaatcgatg
     1141 agatggtaag aacgctacaa ccaaggtatg gcgcatctga aacatacctg aaaaacattt
     1201 aaaaataaaa aaatggacac cacacacttt atttatcaaa acaaac
//
LOCUS       DAZFKM010000413         1245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFKM010000413 DAZFKM010000000
VERSION     DAZFKM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..324
                     /gene="yddL"
                     /locus_tag="ACHKAY_005649"
                     /pseudo
     CDS             49..324
                     /gene="yddL"
                     /locus_tag="ACHKAY_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            363..>1245
                     /gene="yddK"
                     /locus_tag="ACHKAY_005650"
     CDS             363..>1245
                     /gene="yddK"
                     /locus_tag="ACHKAY_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO5704070.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNK"
BASE COUNT          448 a          237 c          193 g          367 t
ORIGIN      
        1 aactcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
       61 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
      121 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
      181 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
      241 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
      301 aacgatgaag ccaacggctc gcgcggcaag aatctctgat tgagttaagt gcccatcaca
      361 atatgatcac tgaccttata ttacacaatc atcccaggat gaaaacaatc actttaaacg
      421 acaaccatat tgcacattta aacgccaaaa acactacaaa actggaatat ttaaacttaa
      481 gcaataacaa tttactgcca accaatgaca ttgatcaact aatatcatca aaacatcttt
      541 ggcatgtatt agttaacgtc atcaacaatg atccacttgc acaaatgcag tactggactg
      601 cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatca ggacttaatt
      661 taaccactca accaccaggg ctgcaaaact tcacctctat caatcttgat aataaccaac
      721 tcacacattt tgatgcaacc aactacgata gactcgtaaa gctaagtctg aatagtaatg
      781 ctcttgagtc aataaatttt cctcaaggca gaaatgtaag tattacacat atatctatga
      841 ataataatgc tctcagaaat attgatatag ataggctttc atcagttact tattttagtg
      901 cggcacataa tcaactagag tttgtgcaat tagaatcttg cgaatggctg caatacctga
      961 atctcagcca taatcaatta actgatattg ttgcaggtaa taaaaatgaa ctcttactgc
     1021 tggatctatc ccataataaa ctaacaagtt tacacaatga cttatttccc aacttgaata
     1081 cgttacttat taacaacaat ttgctttctg aaattaaaat attctatagc aacttctgca
     1141 atgttcagac attaaacgct gctaacaacc agttgaaata tataaatctt gatttcctga
     1201 cttatcttcc atctatcaaa agtttaagac tggacaataa taaaa
//
LOCUS       DAZFKM010000414         1236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFKM010000414 DAZFKM010000000
VERSION     DAZFKM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHKAY_005651"
     CDS             complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHKAY_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO5704071.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..>1236)
                     /locus_tag="ACHKAY_005652"
     CDS             complement(371..>1236)
                     /locus_tag="ACHKAY_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5704072.1"
                     /translation="VSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLP
                     LALISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDP
                     LQGRYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDI
                     WGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETD
                     NSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIG
                     RFIRFVFTGVM"
BASE COUNT          347 a          311 c          225 g          353 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      901 gatcctgagt gatataccgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
      961 ggccggactc ctcatcatac tgctgccccg gcagccggat aagctgctgc agctgatgcg
     1021 ggttctcttc attcagcagg ttgccccatt catcatattc tgcgcaccac gctgttgccc
     1081 cttccgtgct gatgagcgcc agcggcaggc cgcgatggtc gcagtggtac aggtggattt
     1141 ttcgcgccgg cgtgtacacc gggtccatct ggttttgcat ctgctccacg gtcaggccgc
     1201 acgatgccag ccagcggcgg ctttcctcac tcaccc
//
LOCUS       DAZFKM010000415         1199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFKM010000415 DAZFKM010000000
VERSION     DAZFKM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1199)
                     /locus_tag="ACHKAY_005653"
     CDS             complement(<1..>1199)
                     /locus_tag="ACHKAY_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704073.1"
                     /translation="DAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTR
                     LERLNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALA
                     RVTRARERYSRVQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALT
                     RIDKNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMA
                     TAGGMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGP
                     VAGKLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSV
                     SDAGGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLP
                     KLRKELEG"
BASE COUNT          219 a          381 c          315 g          284 t
ORIGIN      
        1 ccttcaagtt cttttctcag tttgggtaag gagccgtcac ctgccgcatc aacgagggcc
       61 ataaatgatg tgaaagcctc ttctccggca atgtccttaa agaacgatac ccggtcaact
      121 tccccgtatt tgcgggtagc tttataaagg tcagccagca catcctccat cggacgcatt
      181 ttgcccccgg catccgagac agacacgcca agctctttca gcgcctctgc tgccgccttt
      241 ggcggtgatg ccagacgagc caggctggca cgcattgccg tcccggcatc actccctctg
      301 atacccatat tcgccagcac gcccgccatc gctgcggcct gctccagcga tattcccagc
      361 ttgcccgcca ccggacctgc atatttcatg gtttcgccca gtgcgcgaag gtcagtgttg
      421 gtacgggtaa atgctgcggt gagcgtgtcg ccaacccggt ccatctggtc agcagaaagg
      481 ccgaactgcg tcaggatatt tgagccaata tccgccgtct cgccgagatc catgccgcca
      541 gccgttgcca tgctcagcac gcccggaagc gcagcctgaa tggcctgcgg agtgaagcca
      601 gccattgcaa gaaatgcctg cccactggcg gcatcgcctg cggtgaactg cgtttcagag
      661 ccaagtttta acgcctgctc acgcagagcc ttaaactgcg ggctgttttt gtcgattcgc
      721 gtcagtgcct gaacgcggga catctctttg ccgaacccga tcgcaggctg taaaaaacgc
      781 ccggcagcat agccgccagc cgctgccgca ccaattgcca gcgcaccacc tgttttcagt
      841 tttcccgcgg tttcctgcac gcgcgaatac cgctcacgcg cccgcgttac acgcgcaagc
      901 gcctgccgtt cgcgttcaag ctggttgttg tactgttcgg tgcgtctgat ggcctgctgg
      961 atggtgttat cgctgcctgt cagggaaatg ccgtggcgtt tcagctctcc gccaagctcc
     1021 cgcattttct gaatttcccg tgtgcgcgat tcattcaggc gttcaagccg ggtgcttaac
     1081 tgctgcatca gcttttgttg tttttcgctg agcactgtac ccgtgcgttg taactgatta
     1141 agggcgttaa gctggcgtcg tgctttcacg atacccgcat ccgctttact gacagcgtc
//
LOCUS       DAZFKM010000416         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFKM010000416 DAZFKM010000000
VERSION     DAZFKM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            427..864
                     /locus_tag="ACHKAY_005654"
     CDS             427..864
                     /locus_tag="ACHKAY_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO5704074.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            884..1196
                     /locus_tag="ACHKAY_005655"
                     /pseudo
     CDS             884..1196
                     /locus_tag="ACHKAY_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          359 a          215 c          293 g          329 t
ORIGIN      
        1 atgtttcaaa aactgaaaga ttggatggtt attttcgtgt gaatgatgat gaagataatt
       61 tattcttaga atggattgta aaagatggtt atttagagga aataattttg tctggttcat
      121 aaattcatct tctttgttga gaagattaac tgcctaggtg aaattatcct ctatgaccgg
      181 gacgctgcag gccacctggc agaagtcatc acgcaggaag acaagtcgct gtatcccttt
      241 gaaaaatccg gcaggctaat gggcatcttc agcccaggcg gcacatcaca gcgaatttga
      301 aggcaaatgc gaaaattagt gatcttgaag ttcatacagt attggtaaaa acaggcgaag
      361 cttttccaat gtgtaagaat tttcaaattt caatatttgg cgctgttgtt acaagcgggt
      421 gattatatgt gtgaattaat taatgaagca tcaaaatatg ggaaagtcaa tgaaagaaag
      481 atggacattg atttgtgctg taaaaaaata gaggaagaag gatattctgt tttcgttgcg
      541 ttctatgatt ttataaaaaa atatggcggt ttatatggct ttcattcagc ttacaaagac
      601 accactataa atgaggcttt ttttatagat ccagaaaggg cctctaatcg catttttata
      661 gaaagagtca atgattatat tgagagagct ggttacaata tgattcctgt tggcgagtgc
      721 agtaatggtc atatcacatt attatatggg gaaaataatg tgatatatgg cggcttggat
      781 gattatcttt ggaagtatgg ggataattta tcttcatcaa ttgatatatt gattaatggc
      841 aaggaggcag ttgaaattcc gtaaggccaa cttgatatga cacctggcac acaaccccga
      901 cgaccggatg tatatgctgg gcgggatgag tgacaccgcc agcaaccgca tcacgtttga
      961 gcgcgacagc cagtaccgga tcacgtgtgt cagtcacacc gacgggatcc ggcttaaact
     1021 gacgtaccat gccagcggct acctgaaagc cattcaccgc acggataacg gcatacagac
     1081 gctggcgacc tacgaacagg atgcgcgggg gcggctgacc gaagcggatg cgcggctgga
     1141 ctaccacctg ttttatgagt acgacgctgc ggaccggatc atccgctggt ccgata
//
LOCUS       DAZFKM010000417         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFKM010000417 DAZFKM010000000
VERSION     DAZFKM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..325
                     /locus_tag="ACHKAY_005656"
     CDS             <1..325
                     /locus_tag="ACHKAY_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5704075.1"
                     /translation="LKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDP
                     HHLIGHGQCGMGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALA
                     IGVLA"
     gene            339..1091
                     /locus_tag="ACHKAY_005657"
     CDS             339..1091
                     /locus_tag="ACHKAY_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO5704076.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     ATLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
BASE COUNT          321 a          235 c          334 g          306 t
ORIGIN      
        1 gctgaaagtg gatccggagt cgccggagtc ttttatgtta cgcccaaaac gtcgccgctg
       61 ggttaatgaa aagtacacgc gctgggttaa gacacagccg tgtgcatgtt gtggaaagcc
      121 cgctgatgat ccccaccacc tgataggcca cggtcagtgt ggaatgggta caaaagcgca
      181 tgaccttttt gtgttgcctt tgtgcagaaa gcatcacgac gagctgcatg cggataccgt
      241 ggcatttgaa gagaagtatg gctctcagct ggagctgata tttcgtttta tcgatcgtgc
      301 gctggcaatt ggcgtactgg cgtaagtgga gaacgagcat gaaccttgaa gccttaccga
      361 aatattactc cccgaaatct ccaaaactga gcgatgacgc accggcgaca ggctctggtg
      421 gtttaacaat tacggatgtg atggctgcgc aggggatggt gcagtcaaaa gcaacgcttg
      481 ggtttgcttt attcctggca aaagttggtg ttcaggatcc tcagtttgcg attgaaggtc
      541 tgctcaatta cgcgatggca ctggataacc cgacattgaa taaattgagt gaagaaaccc
      601 ggctacagat cattccttac cttgtgaatt ttgcctttgc tgattattcc aggtctgcgg
      661 caagtaaggc tcgctgtgag cattgtgctg gtactggatt tcataatgta ttgcgcgagg
      721 tggtgaaaca ctccagaagc ggggaatctg ttattaagga agagtgggtg aaggaactat
      781 gtcagcattg tcatggtaag ggagaagtca gcacagcatg cagagggtgc aagggtaaag
      841 gtattgtcct ggatgaaaaa agaacccggc ttcatggcac gcctgtttat aaggtttgtg
      901 ggcgttgcaa tggaaaccgg tttagccgtt taccaaccac actggcgcgg cttcatgtcc
      961 agaagctggt accagacctg acggattatc agtggtacaa aggatatgca gatgtcattg
     1021 ataaactggt tacaaagtgc tggcaggaag aagcatatgc agagatacaa ttgagaaagg
     1081 tgacaagata aatggttttc gccgaagatg acgacatgat gcttgcattt ttcaaaaaat
     1141 atggataaga ttttcccaac gatgggcttt gtatgtctac cgttgataag atttaa
//
LOCUS       DAZFKM010000418         1195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFKM010000418 DAZFKM010000000
VERSION     DAZFKM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            179..472
                     /gene="iss"
                     /locus_tag="ACHKAY_005658"
     CDS             179..472
                     /gene="iss"
                     /locus_tag="ACHKAY_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO5704077.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHKAY_005659"
     CDS             complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHKAY_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO5704078.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(962..>1195)
                     /gene="rrrD"
                     /locus_tag="ACHKAY_005660"
     CDS             complement(962..>1195)
                     /gene="rrrD"
                     /locus_tag="ACHKAY_005660"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5704079.1"
                     /translation="YIEVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGAC
                     DQLRRWTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          304 a          285 c          272 g          334 t
ORIGIN      
        1 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
       61 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      121 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      181 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      241 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      301 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      361 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      421 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      481 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      541 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      601 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      661 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      721 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      781 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      841 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
      901 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
      961 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1021 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1081 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1141 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat atcgacttcg atgta
//
LOCUS       DAZFKM010000419         1185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFKM010000419 DAZFKM010000000
VERSION     DAZFKM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..191)
                     /locus_tag="ACHKAY_005661"
     CDS             complement(<1..191)
                     /locus_tag="ACHKAY_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5704080.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKL"
     gene            complement(166..732)
                     /locus_tag="ACHKAY_005662"
     CDS             complement(166..732)
                     /locus_tag="ACHKAY_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5704081.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          356 a          262 c          188 g          379 t
ORIGIN      
        1 agtttatctt tctgtttctg ctcctctcgt cgtagtttct tctctgctgc tttttccgct
       61 ttttcgcgtt ctttacttcg tcgttcgagt gctatcttgg ttccacaaat ctcattacac
      121 caatattgat tttgatattt tggtataaac cattcattgc aacatttaca tttccttcga
      181 tagattcgca taagtgctcc tttcgttgcc ggaaaaatca ccgtaatact tatctcgggc
      241 ttcttcagca actagtaccg ctaactccag atcatcaaag catccgaagt gtttactctt
      301 gccatggaat cctagcctaa cattccattt tttctgtcgt ttgtgccaag taacccctct
      361 gcaacctgat ttgctattct ttcggatcct tatatttctt gaattttcta ttggcaggca
      421 ttctcttaaa ttttctggcc tattgtcggt cctaattcca ttaacgtggt caatttgacc
      481 agcaggccaa cgattatgag ttatgtaaaa aactaagacg tgagttttat atctacgccc
      541 atctatcatg atcattgaat aaccgttgga atcaaaagtt ccagcaacac tatttaatgc
      601 tatccttccc tgagtgggaa ctttccatct aaataccccg gtagatttat cgaaacttag
      661 taactcaaat atccttttaa cagttaaatc ttctctttta cggttacatc gtcttcgcgc
      721 tggtttagcc atcgccttct tcctccgtaa tggttttctg aatttggcca cctgaacaga
      781 gctcaccaaa gctatggatg tcggtatttc cacaatacca agatggcgaa aataactgca
      841 tgataagcct cagggaaaag ggaagacact acccccgata attcagaaac aaatcgaaat
      901 acatgaacta aagaaaaaac cacaacaaac agaaatagaa atagaaatag aaatagaaat
      961 agaaatagaa atagaaatag aaatagaaat attaaaacag accaccacat tcctgatgtc
     1021 atactcacta aacaattttt cgacaacatg gtagctcata gaacgttatc gtgtagacac
     1081 cctctgctac gtattcggtg ttcagtgcaa tatctacaaa tactggaaaa atctaaaatc
     1141 gaggcaagtt tttggtgata actatagtta gactatattg acgac
//
LOCUS       DAZFKM010000420         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFKM010000420 DAZFKM010000000
VERSION     DAZFKM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            388..495
                     /locus_tag="ACHKAY_005663"
     CDS             388..495
                     /locus_tag="ACHKAY_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5704082.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            871..978
                     /locus_tag="ACHKAY_005664"
     CDS             871..978
                     /locus_tag="ACHKAY_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO5704083.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(983..>1177)
                     /locus_tag="ACHKAY_005665"
     CDS             complement(983..>1177)
                     /locus_tag="ACHKAY_005665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704084.1"
                     /translation="FRLAADSTTVFLFRNPSLTHSVLRIPQNIIRLQPLHFSYEKGRR
                     VSPLRPGPGANCHYGSLTPA"
BASE COUNT          308 a          271 c          358 g          240 t
ORIGIN      
        1 aagcggaagt agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg
      241 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa
      361 cgttgtcatt acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      421 catgatttag cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg
      481 aacaagcgga agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
      541 gatcaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
      601 ggcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
      661 gaaaaacagt cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg
      721 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
      781 atgcacctga caacatcacg ggggccattc ttgaacctga acaactcaag aatagccgcg
      841 aaacgttgtc attacaacac aggcggctat atgacgctcg cagaactggg catggccttc
      901 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
      961 ctgaacaagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
     1021 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
     1081 gcaagcggat aatattctgc ggaatgcgca gcacagagtg agtaagtgat ggatttcgga
     1141 acagaaaaac agtcgtagaa tcagcggcaa gccggaa
//
LOCUS       DAZFKM010000421         1176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFKM010000421 DAZFKM010000000
VERSION     DAZFKM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..552
                     /gene="dfrA8"
                     /locus_tag="ACHKAY_005666"
     CDS             43..552
                     /gene="dfrA8"
                     /locus_tag="ACHKAY_005666"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HFO5704085.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            549..>1176
                     /locus_tag="ACHKAY_005667"
     CDS             549..>1176
                     /locus_tag="ACHKAY_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO5704086.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAHVKDAIGA
                     SVASKWTAIIHAPVNGVVLGIPDHGFDGKELASYGFGLVLAGHYHNHKK"
BASE COUNT          286 a          312 c          304 g          274 t
ORIGIN      
        1 gacgcaataa tcaccaattc ctcttttgat aggtaaacaa caatgatcga gcttcatgcc
       61 attttagctg ccaccgccaa tggttgcatt gggaaggaca acgcacttcc ctggccacca
      121 ctaaaaggcg atctggccag attcaaaaaa ttgaccatgg ggaaggtggt cattatgggg
      181 cgcaagacct atgagagctt gcccgtcaaa ttagaaggtc gcacctgcat cgttatgacg
      241 cgccaagcgc tggagcttcc gggtgttcgt gacgctaacg gcgctatctt cgtgaacaac
      301 gtcagcgacg ccatgcggtt cgctcaagaa gagagcgtgg gcgatgtggc ctacgtcatt
      361 ggtggcgctg agatattcaa gcgacttgcc ttgatgatca cgcagattga attgaccttt
      421 gttaagcgac tgtacgaagg cgacacctac gttgatctgg ccgaaatggt caaagactac
      481 gagcagaatg gcatggaaga acatgacctt cacacttact tcacttaccg taaaaaggag
      541 cttacagaat gaccttcgtt gccatttcag atacgcacct gcacaactgg tcgcagttcg
      601 cgataccaac agagtcgggc attaactcca ggctgttgca gattcttaaa gccattgaag
      661 aagcggcttg cgccgccgat taccacgccc cagcgggcgt ggttccaacc gtctatcacg
      721 gcggcgatct cttccatgtg cgaggttctt tgaccccttc tgtgctaaac gcggtgctcg
      781 acttttttaa gaccatccac cgtgactacg gcgttcgctt tcgcatgatc gccggcaatc
      841 acgatctgga aaccaaagac tcatgtccta tgggcaatgc agctgcggcg cttaattcgt
      901 tgccgtttgt ggaggttgta tccgaaaaaa cgctgttcga ggaccacaaa gtcgctctgc
      961 tgccatggcg cgattccatg gacgatctgc gtgcggatct tgcgcacgtt aaagacgcga
     1021 tcggcgctag tgttgcctct aagtggacag caatcatcca cgcaccggtt aacggggttg
     1081 ttcttggcat tccagaccat gggttcgacg gaaaagagct cgcctcctat ggcttcgggt
     1141 tagtgctcgc cggtcactac cacaaccaca aaaaaa
//
LOCUS       DAZFKM010000422         1171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFKM010000422 DAZFKM010000000
VERSION     DAZFKM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(393..>1171)
                     /locus_tag="ACHKAY_005668"
     CDS             complement(393..>1171)
                     /locus_tag="ACHKAY_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5704087.1"
                     /translation="TSHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRYILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          275 a          328 c          312 g          256 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgtatcgcca gaaatactca tccacagaca gggcgcggtc atcggcttta
      901 atcagccaca gcaccaggtc cagttcgggc agaatgtcac ggtacagggc ttcatactct
      961 gcatccctgt cccggctctc gcccaccccg ggcaggtcag tgataaccat gctgtgaccg
     1021 tggccactca gacggaagcg ccgcacttcc cgggtgccgg cgtgaacatc actgaccggg
     1081 gtgacctccc cctgaaacag tgcattacag agtgaggatt taccggcccc gcttttaccc
     1141 ataatgccaa tcacgggttc gtgactggtg a
//
LOCUS       DAZFKM010000423         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFKM010000423 DAZFKM010000000
VERSION     DAZFKM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..518)
                     /locus_tag="ACHKAY_005669"
     CDS             complement(<1..518)
                     /locus_tag="ACHKAY_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO5704088.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQL"
BASE COUNT          363 a          269 c          218 g          319 t
ORIGIN      
        1 agctgattct taattgccgc agtcgtggca tcattaaaaa tgaatttata cctcacaggt
       61 tcttccgcac tgtttttctg aagtacttcc agttgtctga gggtttctga ctcatccgcg
      121 cctttgcgga gttctttatt cagttttgac aacaatgctt ttttctgagc ctttaaaagc
      181 tcttccttgt ttttccagac agccagttca cttttgtatc tggaatataa ctgcatctcc
      241 tgctggtaca ggggcttcat taataatttg tccactgcgg tttttcgttc tccggactcg
      301 gcaagcgtca ggaaaaaaag agagaccggc ccccgcagtg tattcagtct gcagacatca
      361 atcagattct ggcagacaag tgaaacagcg cccagggcag acgctgccac aagcccaagg
      421 ggagcctgcg taatttgctg agcttcaata acggcatcac gaatgagtgg aggcagcgaa
      481 ctcaggggaa actcatcaca aggctgaccg aatgacatag taacctcact atacacagat
      541 ataaaagcac tgcaattctg gctattaata gcaaaaatag cagtcaaatc aatatgaggt
      601 aaaatttttt gttgatttaa tggttaaata ataaccacgg tgtattttgc tgctgaagaa
      661 agacaaaatg aatctgtgtg tcccctcctt cccttcggag cacatccact attcaaattt
      721 tacacaacca cgtacgaaca aaaagcatca tcccgggtat tgcagatacc ggtttcctgc
      781 cgggaagaat cataaacaga acaccgggaa taacgccttc aggaatcacc ccccctcctg
      841 aagaagaata aaataaagaa gactacagtt cctgcactga atgctgagta caatatttta
      901 tggacaggat gacattttta taaataacaa aaaccattta atactgatac atcaataaaa
      961 tacttcttcc ctgctgacac agataaaatg caccattttt tcttcaaata cttctgtata
     1021 atcgccagcc aatgatgaga atggattaaa ataacacttc ttcgacaaac acaaaaacag
     1081 ccttcatttt acatcatcat ccagtttcag ttaaccccgg acgagcgaca gtgtttcagt
     1141 acaggaatta aaagccagct tttgctcag
//
LOCUS       DAZFKM010000424         1167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFKM010000424 DAZFKM010000000
VERSION     DAZFKM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /locus_tag="ACHKAY_005670"
     CDS             <1..450
                     /locus_tag="ACHKAY_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO5704089.1"
                     /translation="FGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIY
                     YRMDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMK
                     EKDVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(638..832)
                     /locus_tag="ACHKAY_005671"
     CDS             complement(638..832)
                     /locus_tag="ACHKAY_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO5704090.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          294 a          230 c          296 g          347 t
ORIGIN      
        1 tttggtaaac gccatcagca tgtgattagg gctattaaat gtttgagatg ttctgaggaa
       61 ttctcgacaa cccatttttg ggccgtcgag aaaatcaatg acttaggtat ttttgacaag
      121 aaacagattt actaccgcat ggactttagt ggcttcgtta tgctggttat gggatttaac
      181 ggggcaaaag ccgatgctgt taaagaagcc tatatcaatg cgtttaactg gatgtcagca
      241 gaactccgta agtacagcga aagttatgaa gcagaacgta acgccgtaat gctggagtac
      301 atgaaagaga aggatgtcgc cagcatgtca ggccgtctgc tcaatcgctg ggggagaacg
      361 aaaaaacctc aattgcttgc aaagctggaa cgtctggaga gacagggaca gtttttatta
      421 ccgggattcg ataaaggtat tcaagcctga cacattatgc gctgtatcgt cgccgtattc
      481 ccgcattaac catgaccgta gcccgacggg gaattccttc tgcgtgagtg tgcgggaata
      541 atcaaaaacg atgcacaccg ggttttactg tgctgacaga cgcagggtta ccctcatagt
      601 cgcttttccg gtgcgatggt ggaagaaacc gggatgttca tccatcatca ctttggattg
      661 atgtatatgc tctcttttct gacgttagtc tccgacggca ggcttcaatg acccaggctg
      721 agaaattccc agaccctttt tgctcaagag cgatgttaat ttgttcaatc atttggttag
      781 gaaagcggat gttgcgggtt gttgttctgc gggttctgtt cttcgttgac atgaggttgc
      841 cctgtattca gtgtcgctga tttgtattgt ctgaagttgt ttttacgtta agttgatgca
      901 gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg atggcgtaga
      961 tgcacgttgt gacatgtaga tgataattat tatcattttg cgggtccttt ccggcgatcc
     1021 gacaggttac ggggcggcga cctcgcgggt tttcgctatt tatgaaaatt ttccggttta
     1081 aggcgtttcc gttcttcttc gccgtaacct aatgttttta tttaaaacac cccctgaaaa
     1141 gaaaggaaac gacaggtgct gaaaacg
//
LOCUS       DAZFKM010000425         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFKM010000425 DAZFKM010000000
VERSION     DAZFKM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..107)
                     /locus_tag="ACHKAY_005672"
     CDS             complement(<1..107)
                     /locus_tag="ACHKAY_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO5704091.1"
                     /translation="MSKDFYARLAAIQENLNAPKNQYNSFGKYKYRSCE"
     gene            complement(118..288)
                     /locus_tag="ACHKAY_005673"
     CDS             complement(118..288)
                     /locus_tag="ACHKAY_005673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704092.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            complement(364..516)
                     /gene="kil"
                     /locus_tag="ACHKAY_005674"
     CDS             complement(364..516)
                     /gene="kil"
                     /locus_tag="ACHKAY_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5704093.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(501..635)
                     /locus_tag="ACHKAY_005675"
     CDS             complement(501..635)
                     /locus_tag="ACHKAY_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704094.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(711..1022)
                     /locus_tag="ACHKAY_005676"
     CDS             complement(711..1022)
                     /locus_tag="ACHKAY_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO5704095.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
BASE COUNT          281 a          277 c          239 g          362 t
ORIGIN      
        1 tcttcgcagc ttctgtattt atatttaccg aatgagttgt actgattctt tggcgcgttc
       61 aagttctcct gaatagctgc cagtcttgcg taaaagtctt tgctcatatg tttgttctca
      121 gaatgggcat ggcccaagga aataacgctg atttaatact tcagtctttg ccgcatttaa
      181 aaatacgcga acaccttcac gatctccctt ctggcgatac attaacgcct gctgcgtgta
      241 catgcgtctc tgtaacttgc tctccttcac tgtggttgca agtgacatga atatctcctt
      301 cgttaccgat taattctttc atctgacgaa tgaattcttc gtctgaccag ttatctgtga
      361 aactcatttc ctgcgatacc acggaaggtt gatagctgat ttcatcgctt tatttgcttc
      421 aagccacatt tttgaatcac caataaatct ggctattact gctttgtttt gtgccgcacg
      481 aagcatctgg tgattaatgg ctatttcatt gcgcataacg cctccagttg tttctttgct
      541 gctctaatta attgtttaac tcggcgtgat aattcagatt cgtgcgggta gaaagcggac
      601 atgacgccgc tacccgcgag ctgaaagtgc atcatgggta actccttata tttgattgca
      661 taacgaaaac gcctcgagtg aagcgttatt ggtatgcggt aaagccgcgc ttaggcggct
      721 gatgtttctt ctttcaggct ttcgagatat ttacgtgggt cgtcgtaaca ttggcattcg
      781 ctgtaccaat ccacccagcg atcagtaagc cccacctctg ataaatcttc atcggtaagg
      841 ctctcatccc acatctcaag gccgttagca ttgcagtaat caggcttgat gttgttgtca
      901 tactgaaagg cgtcataatc agccagtgcg cccatcaggc gaacaccctc ttcaacactt
      961 gccacttctg caatgaacgg tttcataggt acttgcggga tatgccagac acgtaatttc
     1021 atatttcctc cgtcaaaaaa atcgccctca cattggaggg caaagaagat ttccaataat
     1081 cagaacaagt cggctcctgt ttagttacga gcgacattgc tccgtgtatt cactcgttgg
     1141 aatgaataca cagtgctta
//
LOCUS       DAZFKM010000426         1157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFKM010000426 DAZFKM010000000
VERSION     DAZFKM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..564
                     /locus_tag="ACHKAY_005677"
     CDS             241..564
                     /locus_tag="ACHKAY_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704096.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            623..865
                     /locus_tag="ACHKAY_005678"
     CDS             623..865
                     /locus_tag="ACHKAY_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704097.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEICDSDGHLLLKVSAPEA
                     GWTHESLNLVQPQEVQEGNDAFDAYLNGIWIGSTEV"
     gene            complement(1065..>1157)
                     /locus_tag="ACHKAY_005679"
     CDS             complement(1065..>1157)
                     /locus_tag="ACHKAY_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704098.1"
                     /translation="NNNIISNRQMTGMKRQKHTAEHPRNERGGA"
BASE COUNT          282 a          256 c          332 g          287 t
ORIGIN      
        1 gactgtccag ggtaaatgaa cagcccacaa aattttttac ctaaaacccg gtaaaaaatt
       61 ttgcgttctg cccaggacag gtgcgtcagg tcgtggcagt gatgcccctt gcggcgaagg
      121 ttctcaaaaa ggtgatggtg aataccggag ggaataaaag gcaggtcccc gcaaaacgtc
      181 aaaaccgcgg ctcccgaaac tcaaaagatg atgggggcaa aaaacagagg tgtattaaag
      241 atgggcagaa atatgtcagc agcacgtacc gatggcttta ttcgtaatat tcattcccgc
      301 aatccctttg atgttatccg ggctgatgtg gtgatcagca ggctggaaaa gcaggctcat
      361 tggggatgtg gtctgcacta tgaaatttat gaagcgaatt tgtttgatat ggcgatgaat
      421 catctttccc ggcttcctct gaaggatcgt ccggtattca ttggtgctgc tgcacgaaag
      481 gggctttttc tgacgatgga agaaaccagg ctggcacggg aagcgaaaga tgctctgatg
      541 gctgaacttg ctctggaata ctgaaaatcg ggccgcaccg tcgtcccgga tagtgctgaa
      601 atatttaatg tggagagaat ggatgggata tatcacatat gttacagatc aacgacccgg
      661 tgaaccagat atcctgaccg ggaacacgtt tgctgatctg gagatttgtg attctgatgg
      721 gcatcttctg ctgaaagtgt ctgcacctga agccggctgg acacacgaaa gcctgaacct
      781 tgttcagccg caggaagtgc aggaggggaa tgatgcattt gatgcttatc tgaatggtat
      841 atggatcggt tcgacagagg tctgaacaaa tcacgccagc agaagacgtt gctggcgtct
      901 tctgcattcc cggggagctg tcgcgcggca tgtttgctta tacccgtgcc ccgagatgat
      961 tatttgtctg cgggcctgaa gacgccgggc aggcgggagg ataaatgccc gtctgcccgc
     1021 gccgggaaaa gacagcacgc cggtggcgct gaaaggggcc gcgcttacgc gccgcccctt
     1081 tcattacgcg gatgttctgc tgtgtgcttc tgtcttttca tgcctgtcat ttgtctgtta
     1141 ctgatgatgt tgttgtt
//
LOCUS       DAZFKM010000427         1154 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFKM010000427 DAZFKM010000000
VERSION     DAZFKM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..567)
                     /locus_tag="ACHKAY_005680"
     CDS             complement(<1..567)
                     /locus_tag="ACHKAY_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704099.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(654..860)
                     /locus_tag="ACHKAY_005681"
     CDS             complement(654..860)
                     /locus_tag="ACHKAY_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO5704100.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          278 a          284 c          324 g          268 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc
      361 ataaatcagc cggtgcagac tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccac ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc
      541 gtaacgggtc tgatactgtg gtttcatttt catattcctc acgggggata acaggcagca
      601 gaacgccgcc ggcaggccgg agactgcgtc tgctgcagat gaacggttaa gaattacgga
      661 cgggactgtg caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag
      721 ctgcggccca ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac
      781 ttatcggtca ggccggtcag ttgagtgata aatgccatgt cgaccatctg gtcatccatc
      841 agtgaaactg gggtgatcat gagaatgttc ctccggatgt tgttaaacca gccggtgtgg
      901 tcatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
      961 tcgggaatgt gaccgccagg agataaacgg ggaggttacg gtggctgagc cgtaccggta
     1021 tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg cgcgataaag aagggatgat
     1081 tttcgtgagg aagaggggaa aactggcgtt ctgataattg cagagttatt tacggggcgg
     1141 ttacgatggg gcag
//
LOCUS       DAZFKM010000428         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFKM010000428 DAZFKM010000000
VERSION     DAZFKM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..863
                     /locus_tag="ACHKAY_005682"
     CDS             <1..863
                     /locus_tag="ACHKAY_005682"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO5704101.1"
                     /translation="KPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSN
                     YGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYTG
                     LSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKET
                     QSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAH
                     GREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASD
                     AVNDLLNTPV"
     gene            873..>1153
                     /locus_tag="ACHKAY_005683"
     CDS             873..>1153
                     /locus_tag="ACHKAY_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO5704102.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          255 a          338 c          356 g          204 t
ORIGIN      
        1 taaaaccggt atgggcgctt gccaacgaca tgaactgcag tgcaggtcag ttgcttgcca
       61 gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc ggcgtcatga
      121 tggctcacag taattacggt gctgcgctgg agaaacaggg tgtggaaatc acgctgattt
      181 acagcggcag ccataaggtg gatggcaacc cctacagcca tcttccggat gacgtccggg
      241 agacactgca gtaccggatg gacgcaaccc gccagatgtt tgcgcagaag gtgtcggcat
      301 ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac agcggtcagg
      361 aggccattga tgccggactg gctgatgaac ttgttaacag caccgatgcg atcaccgtca
      421 tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg accaaagaga
      481 ctcaatcaac aactgtttca gccactgctt cgcaggctga cgttactgac gtggtgccag
      541 cgacggaggg cgaaaacgcc agcgcggcgc agccggacgt gaacgcgcag atcaccgcag
      601 cggttgcggc agaaaacagc cgcattatgg ggatcctcaa ctgtgaggag gctcacggac
      661 gcgaagaaca ggcacgcgtg ctggcagaaa cccccggtat gaccgtggaa acggcccgcc
      721 gcattctggc cgcagcacca cagagtgcac aggcgcgcag tgacactgcg ctggatcgtc
      781 tgatgcaggg tgccccggca ccgctggctg caggtaaccc ggcatctgat gccgttaacg
      841 atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc tttacccatt
      901 accagccgct gggcaacagt gacccggctc ataccgcaac cgcgcccggc ggattgagtg
      961 cgaaagcgcc tgcaatgacc ccgctgatgc tggacaccgc cacccgtaag ctggttgcgt
     1021 gggatggcac caccgacggt gctgccgttg gcattctggc ggttgctgct gaccagacca
     1081 gcaccacgct gacgttctac aagtccggca cgttccgtta tgaggatgtg ctctggccgg
     1141 aggctgccag cga
//
LOCUS       DAZFKM010000429         1146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFKM010000429 DAZFKM010000000
VERSION     DAZFKM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..299
                     /locus_tag="ACHKAY_005684"
     CDS             21..299
                     /locus_tag="ACHKAY_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704103.1"
                     /translation="MNIDEASGCFILRQRIDIVNAARAKAFSRLTVLFCTPDRLSGRD
                     VILLNSDAIQRVCDEFMVANSELFALVQEYNRIARTCGMDELRITHLG"
     gene            380..466
                     /locus_tag="ACHKAY_005685"
     CDS             380..466
                     /locus_tag="ACHKAY_005685"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5704104.1"
                     /translation="MSVLADCVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(560..676)
                     /locus_tag="ACHKAY_005686"
                     /pseudo
     CDS             complement(560..676)
                     /locus_tag="ACHKAY_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(747..1082)
                     /locus_tag="ACHKAY_005687"
     CDS             complement(747..1082)
                     /locus_tag="ACHKAY_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO5704105.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          288 a          194 c          289 g          375 t
ORIGIN      
        1 attcatatgg tgtgggtaga gtgaatattg atgaggcgtc ggggtgtttc atccttaggc
       61 agcgtattga tatagtcaat gcagcacgag caaaggcctt cagccgtttg acagttttgt
      121 tctgtactcc tgatcgtctt tcgggaagag acgttattct tctgaatagt gatgctatac
      181 agagggtttg cgatgagttc atggttgcta attcagaatt atttgctctt gttcaggagt
      241 acaacagaat agccaggacc tgtggtatgg atgaacttcg gattactcat ctggggtaga
      301 tacatatctg gattatcacc tgttacggta aaaagtgatt gcttactgtt tttgtgaatg
      361 gcattgcagc agccggataa tgtcagtgct ggctgactgt gtactggtgg cgggtgtggt
      421 ggttgttgct ttcccgttgc tgaaaaagaa aacgccagac tgttagccgg gtatcagtta
      481 gcgggagaaa tttttaaata cttcacaatt caggcggttg actgttgtct ggtttgcggg
      541 gagtttgtta aaaaaaactg gcatggtgaa tccccctgtg cggaggggca atcagcgagt
      601 aggtatatgg gataatcgcg gattcaggtg ctggtactga attcaccggg aggcacccgg
      661 caccatgcaa tggcacatag cgccactctc cagcccctct ccggaggggc tgtttatatt
      721 gattttgtca gatgtgagta aactccttat ggactttgtt gttttagtcc ataaggacat
      781 atttgcagag tgcaacggtt attaaagcat tcattcaata cgttatctgt atttgtaggg
      841 cattcctggc tgtttttgat taaattccag aatgttttat tgaatggtac tacgttgtaa
      901 atggttacag gtagcacttt gttattgagc atgatgcctg tgtgagtcag tgtaaatata
      961 ctttcaggag gtaagaaagc atccgattga taccagatta ttaattttat tttactccat
     1021 atgactgaaa aagatattcc gcatgatggc tggataactg tatcaatcac aatccacttc
     1081 atttactttc cttgtttatg ccttgctggt gatgttctga aaagtataaa tgatattttt
     1141 gaatta
//
LOCUS       DAZFKM010000430         1146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFKM010000430 DAZFKM010000000
VERSION     DAZFKM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHKAY_005688"
     CDS             <1..249
                     /locus_tag="ACHKAY_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5704106.1"
                     /translation="AGDPDTLLGLPVEYLEFMPDNVIALGDFKRGYYIVDHETGVRTR
                     PDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            289..861
                     /locus_tag="ACHKAY_005689"
     CDS             289..861
                     /locus_tag="ACHKAY_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5704107.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            863..>1146
                     /locus_tag="ACHKAY_005690"
     CDS             863..>1146
                     /locus_tag="ACHKAY_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704108.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQT"
BASE COUNT          274 a          281 c          341 g          250 t
ORIGIN      
        1 gcgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt tatgcctgat
       61 aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca cgaaacaggt
      121 gttcgcacca gaccggacaa cctcacagag ccgggcttca tcaaaatttt cacgcagaaa
      181 tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggaact gccacaggac
      241 gacgattaac agcatacaga aggggcttaa aagccccttt agtgttttat gggtgaaaaa
      301 attatgaaga gtatggaaat ccggtcatcg gaaatcacca ccagcgccag caacacgctt
      361 acaggctacg ttgttcgctg ggataagctt tcagagctgc tatgggggga gttttacgaa
      421 aaattccagc ggggagcgtt tactgaatgg cttgcggcgg gtaatgacgt tcgcggcctg
      481 tatgagcatg accacagcat gttgctgggg cgtacccgtt ccggcacgct gaaactggaa
      541 gaggacgaaa cagggttacg ctttgaactg accccaccgg ataccagcac agggcgggac
      601 gttatcgaac tggttaaacg tggtgatata tccggcatga gctttggctt tcgttcccgt
      661 aaggatgtat gggataccac aacagatcca tgcgtgcgca ccgtgctggt tgcggaactg
      721 tacgaaatta ccgtcacatc cgtaccggct taccctgatt ccggcgtgga gctggcccgc
      781 cgctccctgt acgagcagca ccccgaaaaa atgccgcgtg cggataatcg ccgctggtgg
      841 gcggatttag cgggggtgtg atatgtggcc tttcagaaga aaaaaagagc agcgcagcat
      901 gacgcttgat gagtttatgg cgctggctgg cacatcgaac acgggggcgg gtgagtacgt
      961 atcatcgggg acagcggaat cactgcccgc cgtcatgaac gccgtgacgg tcatctctga
     1021 ggcggtggct accatgccgt gttacctgta cctggtacgc aatgagaagg ggaaggaggc
     1081 ccgcgagtgg cttgattctc atccggtcga tcacatcctc aacgagcgcc cgaacgcatg
     1141 gcaaac
//
LOCUS       DAZFKM010000431         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFKM010000431 DAZFKM010000000
VERSION     DAZFKM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1140)
                     /locus_tag="ACHKAY_005691"
     CDS             complement(<1..>1140)
                     /locus_tag="ACHKAY_005691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO5704109.1"
                     /translation="TQYGGPIVQGSAGVRIGAPTGVACSVCPGGMTSGNPVNPLLGAK
                     VQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDIRLQIRDDALILN
                     DNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWASLPPDIRLSPHL
                     YLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGRTLAYRREAAGDL
                     AGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPLSAPSFPDTLPGT
                     EYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYDRSGTQMRHFTYD
                     DKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQ"
BASE COUNT          201 a          392 c          349 g          198 t
ORIGIN      
        1 tcctgctcat actggtagcg gtaactcagg cctgccgggt taagttgctc caccacccgc
       61 cccgcatcgt cgtagcggta gcgcatctcc ggcctgcctg cataacggtg ccccaccatc
      121 cggccccggt atttatcatc gtaagtaaag tggcgcatct gcgtaccgct gcggtcatac
      181 accgccgcca gctcaccgcg tggcgtccag tcgtagcaca ccagtggcgc ggcaggtaaa
      241 ttttccgggt attccgggtc gtgcatcagc cacaccgccg aaagacggat accgctgtca
      301 gcgccgtatt ccgtaccggg cagtgtgtcg gggaaggaag gagctgatag agggcgggga
      361 ctgtcagggg aagataacga agcggtgtgc ggtttacggg cctcttccgc ccgctgtgcc
      421 tgtgtggtca gtaccagacg gaactcccgc ccggcaccgt ccgtcacgcc ggtgatttcc
      481 ccggccaggt caccggcggc ctcacgccgg tacgccagcg tccgcccgaa gcggtccgcc
      541 atcccggtaa gcacccggta cggcggcagc ggcgctggca gtaagtcctc agcacccggc
      601 acccgctcag accaccccag tatccaccac ggcccctgtg cgctgttggt cgccaggtaa
      661 agatgcgggc ttaaccggat atccggtggc agcgacgccc acagccgtgc cagcgtgtgg
      721 ccgtccggct gcgctgcctt accgccacgc accagccaca ttgactcact gcggctgtac
      781 accgcctccc ccggcagcag cggctcaaag tgaatgctcc gcccgccgtt gtcgttgagt
      841 atcagtgcat catcgcgtat ctgtaagcgg atatcagaag gcgctttcca gccggggccg
      901 aaaacgccca ccggtgcagg cgtcttcgtc cggtagctgc tgtaggtgcg ggagagaatg
      961 aacggcagcg ggccgggcag cgcaaggtcc gtctcgccgg gctgtacttt tgcaccgagc
     1021 agcgggttca ccgggttgcc cgaggtcatc ccgcccggac acaccgagca cgccacgcca
     1081 gttggcgcgc caattcttac acctgccgaa ccctggacaa tgggaccgcc atactgagtc
//
LOCUS       DAZFKM010000432         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFKM010000432 DAZFKM010000000
VERSION     DAZFKM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..326
                     /locus_tag="ACHKAY_005692"
     CDS             <1..326
                     /locus_tag="ACHKAY_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO5704110.1"
                     /translation="REDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASV
                     ANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGICNDLAA
                     ASACM"
     gene            323..904
                     /locus_tag="ACHKAY_005693"
     CDS             323..904
                     /locus_tag="ACHKAY_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5704111.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGYDMAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          256 a          287 c          349 g          238 t
ORIGIN      
        1 agcgtgagga tgactggtgg cgcaacggtc agaacctgta tctggacaat atggcggtca
       61 ccggctttta ccgggtgccc ctgtcctctg cacaggcggg cgatatcctg ctgtgctgct
      121 ttggcgcatc ggtggccaat catgccgcca tttactgcgg caacggtgaa ctgcttcacc
      181 atctgcctga acaactgagt aaacgggaga ggtattccga aaaatggcaa cgacgaacgc
      241 attctgtctg gcgtcaccgc cactggcacg catctgcctt cacggggatt tgcaacgatt
      301 tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa gccatccgtg ccctgtcgat
      361 gcagatgccg ggattccgcc gtcagatgaa cgaaggctgg taccagatac gtattgccgg
      421 ttatgacatg gcaccggagg cggtgtatgc ccgccttcac gaacaactgg gtgagggaac
      481 ggtcatccat attgtgccgc gactggccgg tgcagggaaa ggcggactgc agattgtgct
      541 gggggcggca gccatcgtgg gctctttctt caccgccggc gcaacgatgg cgttatgggg
      601 ttcagccctg gcagccggcg gtttttctgc caccacgatg ctgttttcac tgggggccag
      661 catgatactg ggcggtgtgg cccagatgct ggccccgaag gcaaagacgc cggagtacaa
      721 aagtacggat aacggtaaac agaacacgta tttttcgtca ctggacaaca tgattgccca
      781 ggggaacccg atgccggtgc cttacggtga aatgctggtt ggctcccggc gaatctccca
      841 ggacatcagc acccgtgatg aaggtggtgg cgggaaggtg gtggttatcg ggcggcaggg
      901 gtaaagcata aaaaaatccc gcagtgtatg gaggctgcgg gaacagaaaa tgaagattaa
      961 ccacaggggg ttttgttttt attggcccga aaaaactgta acgcccggga atgatatctg
     1021 ccacggggcg tacagaaaat gtgaagaaat tcagaaattt tattccgtca tgacacaggc
     1081 accctccggg gtgcctgtcg tttttggggc ataaacagat tcagacatca
//
LOCUS       DAZFKM010000433         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFKM010000433 DAZFKM010000000
VERSION     DAZFKM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..277
                     /locus_tag="ACHKAY_005694"
     CDS             26..277
                     /locus_tag="ACHKAY_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704112.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHVGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            274..579
                     /locus_tag="ACHKAY_005695"
     CDS             274..579
                     /locus_tag="ACHKAY_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704113.1"
                     /translation="MSEPKFGEKLYKHNGRITIQQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            589..999
                     /locus_tag="ACHKAY_005696"
     CDS             589..999
                     /locus_tag="ACHKAY_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO5704114.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            1012..>1127
                     /locus_tag="ACHKAY_005697"
     CDS             1012..>1127
                     /locus_tag="ACHKAY_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5704115.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMQLVETDKERHH"
BASE COUNT          344 a          254 c          335 g          194 t
ORIGIN      
        1 gagcaaaagt ggggagtagt ggggaatgaa cagaaaaacc agagataaga cagcgccaaa
       61 atataaagcg ttagacatga cagagctttc cctaaaggtg gcaatcagaa cgatagaccg
      121 acacgtaggg gaaggatacg cgaaggaaca tcccgacctg ataagcgcat tcatgaccac
      181 agcagcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg aacaggtaac
      241 aaccatcaac gttaaaaccg gagaggtgga atcatgagcg aaccaaaatt tggtgaaaaa
      301 ctttacaagc acaacggacg gatcacgatt cagcaaataa gcgcagctac accaggctgg
      361 tgggttgaaa cagacgaagg atcatcaccc gtggcatcat gggcgctttg tgtgcttagt
      421 taccctgacc gcgatgtgta tcaggatatt ctgcctgtga tatccactga caaaggcatg
      481 aaacccgtag acatcaaaaa catgggtttt cagtgcgtaa tgctcactga aaaaatgatg
      541 gaagagatga aaaagaacaa cgccggatcg cttcactgag gtggcaatat gacagcacaa
      601 attgcagctt acgggcgcct ggtggacgac ccgctggtaa aacagagcag caagggcaca
      661 ccgatgactc tggcgcgtat ggcggtatcg ttgccatgca gccaggcaca tgacggacag
      721 gcgacgttat ggttatcggt tatcgcattt ggcaaacagg ccgatttcct ggctaaacat
      781 caaaaaggcg atgttgccag cgtatccggc acgatgcagg taagccagtg gaccggacag
      841 aacggggaaa cacggcaggg gtatcaggtt attgcagaca gcgtaatcag tgcccgtgcg
      901 gcacgtcctg gcgggaacag acgcaaaacc acaggcacac agggtaatca gccaccagcg
      961 ggaggcgatg acccttacgg tgatgatatt ccgttctgag gggtggggac gatggtacat
     1021 gaccgcatag cggaagagct ggaggcgaaa ggcttttacc ggagagcgtc ggcgcgatgg
     1081 ggtgaagtca tgcagctggt ggagacagac aaggaacggc atcacat
//
LOCUS       DAZFKM010000434         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFKM010000434 DAZFKM010000000
VERSION     DAZFKM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..779
                     /locus_tag="ACHKAY_005698"
     CDS             <1..779
                     /locus_tag="ACHKAY_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO5704116.1"
                     /translation="TRHEPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRR
                     FRLSGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEY
                     FWRHILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPV
                     HPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAV
                     DRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          247 a          308 c          302 g          270 t
ORIGIN      
        1 tcacccgtca cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac
       61 tctgtaatgc actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc
      121 gggaagtacg gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc
      181 ccggtgtggg cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc
      241 tgcctgaact tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg
      301 atgagtattt ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga
      361 cgcaggccga caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc
      421 ctgcacaggc acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg
      481 tacatccggt tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg
      541 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg
      601 agctgcgcac ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc
      661 ggatatttga cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg
      721 ctgttcgtga cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa
      781 cctgtcgtga ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt
      841 actgtctctc ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt
      901 atcgatcgtt tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat
      961 acgtacagaa agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga
     1021 cctccggcac tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta
     1081 ttcaccctca atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZFKM010000435         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFKM010000435 DAZFKM010000000
VERSION     DAZFKM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.441"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..246)
                     /locus_tag="ACHKAY_005699"
     CDS             complement(25..246)
                     /locus_tag="ACHKAY_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704117.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(236..427)
                     /locus_tag="ACHKAY_005700"
     CDS             complement(236..427)
                     /locus_tag="ACHKAY_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO5704118.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            complement(458..928)
                     /locus_tag="ACHKAY_005701"
                     /pseudo
     CDS             complement(458..928)
                     /locus_tag="ACHKAY_005701"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            927..1100
                     /locus_tag="ACHKAY_005702"
     CDS             927..1100
                     /locus_tag="ACHKAY_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704119.1"
                     /translation="MSTPLEKRSPPYQMRLPEDFRAQLEEAAKADGDTSLATWIKRVL
                     RKELQQRGIEPKG"
BASE COUNT          288 a          277 c          298 g          264 t
ORIGIN      
        1 tgtttcaggg aattaaaacg atcatcacgc ataacccccc ccattttcac aatcagcaac
       61 aagaatattt ttagcgtcat tcagcgaccg cgttgcgatg tatcggatac attccagggc
      121 gaactgtgtc cggtctttct gttcttctcc ctgcgcaagc tctgcagtgc attcaatatc
      181 gataagcgcg tgcatcagcg tagtgagcgc ggcggctgct gcgtccggtg tggttttatt
      241 gcacatggca cacctcctga cgaatacggg cggcgaatac agcaacacaa ccggacgggc
      301 aacggctacg cgcttcgcgt tccgtccagg cggttacgtg gatgatttga tgttctgacg
      361 cgccaaagat gataaagcgc cagagaaagg cggtttgaga atgtacaagg gtagggatag
      421 tagccatgtt ggcagcctcc tcagtgtggt taagataacc gccaccggaa acgccaattt
      481 cgctggtggc ggactgtaca gggttggcgt aaccggacac tgaagaaacc ggcgcggatt
      541 gctccgcccc cgtacagccc accattgata aggtgtgact atgcggcaca aaaaaacacg
      601 cggggcgcgt gttatgcgct tcagttttgc ccaggacgcc aatcccggca cccgttttat
      661 gaggtgcgcg ggaatcatag ctattttcga cactggcgat caactggttt ttttctccga
      721 tattcagggc cagaaaacgc cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat
      781 gatttacggc aaccataacg gctcctagtt tacgttgttg gttagacgcc ccgtatgtgt
      841 tcccagcact gcggggcgtt gatttttgtg taatggcaag tacaatggta attaccatgt
      901 aggtatagta atgaagggta attaccatgt caacaccatt agaaaaacgt tcgcctccat
      961 accaaatgcg attaccggaa gacttcagag cgcagctaga agaagcagct aaggccgatg
     1021 gcgacacatc tttagccact tggattaaac gcgttctccg caaagaatta cagcaacgcg
     1081 gcatagaacc caaaggataa aaactatcag cgccgtggtg tgaggta
//
LOCUS       DAZFKM010000436         1122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFKM010000436 DAZFKM010000000
VERSION     DAZFKM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..633
                     /locus_tag="ACHKAY_005703"
     CDS             61..633
                     /locus_tag="ACHKAY_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5704120.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSASGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            694..>1122
                     /locus_tag="ACHKAY_005704"
     CDS             694..>1122
                     /locus_tag="ACHKAY_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO5704121.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQR"
BASE COUNT          235 a          286 c          365 g          236 t
ORIGIN      
        1 atggcacgca tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt
       61 gtgaaaacgg gggctgaagc catccgcgca ctggccacac agctcccggc gtttcgtcag
      121 aaactgagcg acggctggta tcaggtacgg attgccgggc gggacgtcag cgcgtccggg
      181 ttaacggcgc agttacatga gactctgcct gatggcgctg tgattcatat tgttcccaga
      241 gtcgccgggg ccaagtcagg tggcgtattc cagattgtcc tgggggctgc agccattgcc
      301 ggatcattct ttaccgccgg agccaccctt gcagcatggg gggcagccat tggggccggt
      361 ggtatgaccg gcatcctgtt ttctctcggt gccagtatgg tgctcggtgg tgtggcgcag
      421 atgctggcac cgaaagccag aactccccgt acacagacaa cggataacgg taagcagaac
      481 acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc tgttctgtac
      541 ggggaaatgc gcgtggggtc tcgcgtggtt tctcaggaga tcagcacggc agacgaaggg
      601 gacggtggtc aggttgtggt gattggtcgc tgatgcaaaa tgttttatgt gaaaccgcct
      661 gcgggcggtt ttgtcgttta tggagcgtga ggaatgggta aaggcagcag taaggggcat
      721 accccgcgcg aagcgaaaga taacctgaaa tccacgcagc tgctgagtgt gattgatgcc
      781 atcagcgaag ggccgattga aggtccggtg gatggattaa aaagcgtgct gctgaacagt
      841 acgccggtgc tggacagtga ggggaatacc aacatctccg gcgtcacggt ggtgttccgg
      901 gcaggtgagc aggagcagac accgccggaa ggttttgaat cctccggctc cgagacggtg
      961 ctgggtacgg aagtgaaata tgacacgccg atcacccgca ccatcacgtc ggcaaacatc
     1021 gaccgtctgc gctttacctt cggtgtgcag gcactggtgg aaaccacctc aaagggggac
     1081 cggaatccgt cggaagtccg cctgctggtt cagatacagc gt
//
LOCUS       DAZFKM010000437         1118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFKM010000437 DAZFKM010000000
VERSION     DAZFKM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1118
                     /locus_tag="ACHKAY_005705"
                     /pseudo
     CDS             1..1118
                     /locus_tag="ACHKAY_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          247 a          246 c          382 g          243 t
ORIGIN      
        1 tcgtcaggca cacgccagga gggcgcgtct gacggtaata aggccataag cgaaacagag
       61 cgtgatgcgg tgaaggcgat gttcaggggg atactgcgaa aatgacagag cgtgaactgt
      121 ccattatccg tgcgctgggt gaagaattct ccacggtgct ggcggattta cagcgcacat
      181 ttgaggggaa gatggcctcg caggcacaag cgtttgaaga gaaactgact tccctgtcgg
      241 cggtattaca gaagcatgtg acggtggatg aggtgcgtcc ggttctgcag gtgatggtgg
      301 atgacgctgt gggggccatt ccggtaccgc gtgatggtcg tgattatgat ccggatgtac
      361 tgcagcaggc ggtgaatgat gcggttgcga atattccggt accggcggac ggcaaaagta
      421 ttacccccga tgatgtgcgt ccgatgcttg aacagatggt gaaggaggcg gtaagccata
      481 ttcctgttcc gcgtgatggt cgtgattacg atccggaagt actgaagcag gcggtgaatg
      541 atgcggttgc gaatattccg gtaccggcgg acggcaaaag tattaccccc gatgatgtgc
      601 gtccgatgct tgagcagatg gtgaaagagg cggtaagcca tattcctgtt ccgcgtgatg
      661 gtcgtgatta cgatccggaa gtactgcaga aggcggtgaa tgatgcggtc gcgaatattc
      721 cggtaccggc ggacggcaaa agtattaccc ccgatgatgt gcgtccgatg cttgaacaga
      781 tggtgaaaga ggcggtaagc catattcctg ttccgcgtga tggtcgtgac tatgatcccg
      841 atgttctgca gaaggctgtt ctggaggcgg tgaatgccct gccggctccg caggacgggc
      901 gtgatgccac ggctctggaa gtgctccccg ctattgacga tcaaaaatcc tttccccggg
      961 gcacgtatgc cacacacctg ggaggattct ggcgggcgta tgaaaaaacg cacggaatgc
     1021 ggggatggga atgcctggtt gacggggtgg cggatattga cgtcagtatg acgggggagc
     1081 ggttgttctc tgtggtgatc cggcacagca gtggccag
//
LOCUS       DAZFKM010000438         1116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFKM010000438 DAZFKM010000000
VERSION     DAZFKM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..375)
                     /locus_tag="ACHKAY_005706"
     CDS             complement(<1..375)
                     /locus_tag="ACHKAY_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO5704122.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSN"
     gene            556..708
                     /locus_tag="ACHKAY_005707"
                     /pseudo
     CDS             556..708
                     /locus_tag="ACHKAY_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(733..>1116)
                     /locus_tag="ACHKAY_005708"
     CDS             complement(733..>1116)
                     /locus_tag="ACHKAY_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704123.1"
                     /translation="FKSSIRNIFYPSRLERLSRFQFIIYTLTSWSILITLNDINLALS
                     FILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQL
                     NVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          360 a          190 c          211 g          355 t
ORIGIN      
        1 attgctgcca tttccatatc taaaatgttc aatcagatct tcgataagtg ttttatattg
       61 tccaaaaaag gaaagcattt ttgctagttc tttcatttct gcaaataaaa tatcagtgca
      121 ttcacgttgt gatatttttg ttccggaact tgaggcacta aaagcaacat taatagaacc
      181 tgggccaggg aaatggtatt taataagtct gtaaggatca acttttgcag agatatcgct
      241 taggccgagt gagagaaagt cttgttctac catatcacca tattgcatat catcagcaga
      301 aaaatcatcg aataaactaa tagtttcgaa aagtgtacag ggaatattaa ttggcggaag
      361 taatatgtta ttcatttgca tttgccttgg ttatatttaa gctcaatatc aattcgagaa
      421 caaatgctag ggtaaaaaaa tatttaaaaa agaagtaaac ttcacatttt ttcattacat
      481 ctctgaagag aacctcgtta atgattggtc ttgctcattg ttaataacaa aatgttgaat
      541 ttaccgatta ggaaactttt tcctacgcgg attgcctcca gctatggcat gaactttgag
      601 tttatgccag aactgaagtg gggcttcggc taccctggcg cgattatctt tatgatcctc
      661 gcgggcctga caccgtatct gtactttaag cgtaagaact ggctgtaact atacgatgtc
      721 gctgtgagta agttagtgtg ctgacagcga cattttagcc gagggaatcg cagataacat
      781 aattgttgtg aataatatcc atgccacgtt gagttggacg cttaatgact cacatctcac
      841 cccttcaatt gctgcagaga taatagaaaa aagtgtcaag cccaatgaaa tcaatacaaa
      901 gtagattgcc ttacatccag tatctcttat tctttttgct ccaatattga tatttacata
      961 ggtattatta ataaccagaa taaaactcaa agccaggttg atatcattca aagttattaa
     1021 tattgaccat gatgttaatg tatagattat gaactgaaag cgtgataaac gctcaagcct
     1081 ggatgggtag aatatatttc tgatggatga tttgaa
//
LOCUS       DAZFKM010000439         1091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFKM010000439 DAZFKM010000000
VERSION     DAZFKM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..802)
                     /locus_tag="ACHKAY_005709"
     CDS             complement(113..802)
                     /locus_tag="ACHKAY_005709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO5704124.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHKAY_005710"
     CDS             complement(817..939)
                     /locus_tag="ACHKAY_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO5704125.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          303 a          267 c          198 g          323 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 ttatattgct atatagaata gccattacta atgcttttaa atttaataaa ataaaaatta
     1081 taaaaaacat a
//
LOCUS       DAZFKM010000440         1084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFKM010000440 DAZFKM010000000
VERSION     DAZFKM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1084
                     /locus_tag="ACHKAY_005711"
                     /pseudo
     CDS             1..1084
                     /locus_tag="ACHKAY_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
BASE COUNT          384 a          204 c          203 g          293 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacctggaat atacaaaaac atggggagta atagcgttaa
      961 cattattatt aataataccc gaaaaattat cccccctggt aaagtattta cgcttagagg
     1021 cggaactcta aatatcaata ttcctgggcg tagcgctctt cttttaggga aaacaggaga
     1081 accg
//
LOCUS       DAZFKM010000441         1081 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFKM010000441 DAZFKM010000000
VERSION     DAZFKM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1051)
                     /locus_tag="ACHKAY_005712"
     CDS             complement(<1..1051)
                     /locus_tag="ACHKAY_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5704126.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREW"
BASE COUNT          217 a          282 c          249 g          333 t
ORIGIN      
        1 cccactcccg cccgcgactg tccctgtaac gcgccgccat tgaatctgat ttatgcccga
       61 gaagacgttg agcaaactta tcgccaatct gattccggta tagcctcgcc gacaggctgc
      121 gcagttcatg gaatgttggc gggtctccat caaatgagag tccagatgca tttctcgcct
      181 ttgtaaaata cttagatact gttttcgggg aaagcggttc gtgatgcgtt gatgcaatta
      241 ttgtttcact gccgctggcc tccctgcatt tctgtagtgt atcagccaat gagatattga
      301 gcgcgtcaat cgttagcgtc agcggaatgg cgagcttagc ccctgtttta ccctgctcaa
      361 tgtgaagatg gttgtcgttt atgtctgacc atttcattct gcataaatcg cctactctct
      421 gccctgtaac gacagccaaa tccattgcca gcctcagcca gattgggagc ggttcggctg
      481 catggtaaat cgcgacatat tcattagctg tcagtcttga acgccttact tctgattttg
      541 ctgtgcgggt tgctgttacc ggattcgtag ccacatgccc ctcggctatt gcctcacgaa
      601 aaacgtcaac aagggttgac ctgattaatt ttgcggaagc cgctttacct tctgctacgt
      661 aggtgtttag cattgctgcc acctctttcg ttgatatgtc agcgagcggt ttgtccggca
      721 attttcttcg gattgccctg attttgctgg cgtagtcgag tagagttttc ggcctgatac
      781 ccctctcgct gaggattgtt tcatatcggt caagccacgc atgaagagtg attgcgtcag
      841 cgcctttaat tctgtctatc agtgactcac gcctgttccc ggatagcaac tcaatattgg
      901 cctgtattgc ttcagtgatt gctatcctcc tgtctcggcc taatccgaac tctttacccg
      961 tccttgggtc cctgtagcag taatatccat tgtttcttat ataaaggtta gggggtaaat
     1021 cccggcgctc atgacttcgc cttcttccca tttctgatcc tcttcaaaag gctacttgtt
     1081 a
//
LOCUS       DAZFKM010000442         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFKM010000442 DAZFKM010000000
VERSION     DAZFKM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..174)
                     /locus_tag="ACHKAY_005713"
     CDS             complement(<1..174)
                     /locus_tag="ACHKAY_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5704127.1"
                     /translation="MSIKHYDVIRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPS"
     gene            151..294
                     /locus_tag="ACHKAY_005714"
                     /pseudo
     CDS             151..294
                     /locus_tag="ACHKAY_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(489..656)
                     /locus_tag="ACHKAY_005715"
     CDS             complement(489..656)
                     /locus_tag="ACHKAY_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5704128.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(653..1076)
                     /locus_tag="ACHKAY_005716"
                     /pseudo
     CDS             complement(653..1076)
                     /locus_tag="ACHKAY_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          275 a          312 c          234 g          255 t
ORIGIN      
        1 tgaagggccg acctgtggct ctccttcaat agccaccgcc tgcatcagtg tgtacggcgt
       61 aatggcaacc ggtccgccgt atggctgcca gccctctttc agtttgtgtg tcagcttttc
      121 cgcaaggtct gacggcgacg ccgccctgat aacatcgtaa tgtttaatcg acatcgaatt
      181 tctcccgtgt agaggaacag agttaaaaag ccggaagcgg aatcaaatca caggatgacc
      241 atctgccagt ggctggtcgt aaaaaaaagg ccgcgccatg cgcagccgaa aataaaggga
      301 taacgatgat agtttgagaa aaacagaaat aacacttttg tggcaaagca tggtgccggg
      361 tgcctcccgg tgaattcagt atcagcacct gaatccgcga ttaccccata ttccttcttg
      421 ctgattgccc caccgcacag ggggattcac catgcagaag tgtttttaat aaacagcaaa
      481 caaaaaaatc aagcattatg caggctgttt ctttttatca ccggccacag caataccata
      541 atgccgcaga ccagcacccc atccgccagc accgacatga ttctgctggt gaaatccacc
      601 atcaccacca gaaacagcag gagtgcagcc acagtcaggc gcagttttac cgtcacaggt
      661 aattctccag acgaagaccc agaacaccgg caatctcttc cagcaccttg cgctcttccg
      721 gctcaatttc gccgtctgcc tccgcaatgg ccaccgccac atccagcaca tcttccgctt
      781 cacgcgtatc gtgtttcaca tcctcgatct cacgtaacgc cgcacgacga ccagttttaa
      841 agttcgtatc cagctgaccg ataatggttg cgctaatcgc attaatttct gacgtaaacg
      901 cgtacagcgc aggctgatta cgcagtacct gttcgatctt cgctttctag gaagcctcac
      961 attcaccatc tgcataggcc accaggtagg cggcgttaat caccacctgt gccagatcgc
     1021 gtttttcaaa ctttttaatt tccgttgccg ctctgcgggc tttttttacc aaaaat
//
LOCUS       DAZFKM010000443         1066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFKM010000443 DAZFKM010000000
VERSION     DAZFKM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..663
                     /locus_tag="ACHKAY_005717"
     CDS             <1..663
                     /locus_tag="ACHKAY_005717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO5704129.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
     gene            666..992
                     /locus_tag="ACHKAY_005718"
     CDS             666..992
                     /locus_tag="ACHKAY_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5704130.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..>1066
                     /locus_tag="ACHKAY_005719"
     CDS             1002..>1066
                     /locus_tag="ACHKAY_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704131.1"
                     /translation="MAISAGRLTQMISVLNPVLTR"
BASE COUNT          233 a          256 c          356 g          221 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 tgaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaa
//
LOCUS       DAZFKM010000444         1063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFKM010000444 DAZFKM010000000
VERSION     DAZFKM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1063
                     /locus_tag="ACHKAY_005720"
     CDS             <1..>1063
                     /locus_tag="ACHKAY_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5704132.1"
                     /translation="IVDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFIL
                     IPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDD
                     LCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLRE
                     AIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPE
                     MVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGL
                     ALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETRE
                     WLGWGHAWVHETAVVRRKSE"
BASE COUNT          218 a          267 c          357 g          221 t
ORIGIN      
        1 attgtggatg caccgggtag cccgacattc ggggagtcct gtgcaccgtg ggtgtttgac
       61 ctggtggcgg ccctgtttgg ctcctacgat gcgcagaccg gtgttcgcca tatcaaggaa
      121 gtgtttatcc ttatccccaa gaaaaacagt aagtccacgc tggccgcggg gatcatgatg
      181 acggcgctgt tactgaactg gcggcaggcg gcgggctaca ccattctggc cccgaccgtg
      241 gaggtggcgg ctaacgcctt caaccctgcc agggatatgg tacgacggga cgatgatctg
      301 gatgacctct gtcaggtgca gacacatatc cggaccatca cccatcgggt gacggacacc
      361 accctgaagg tggtggcagc cgatccgaat acggtgtccg gtatcaagtc cgtggggaca
      421 ctgattgatg aactgtggct gtttggcaag cagtacaagg cggaagacat gctacgtgaa
      481 gccatcggcg ggctggcttc acgtccggaa gggtttgtgg tgtacacaac cacccagtcg
      541 aatgaaccgc ctgccggggt gttcaggcag aaactgcagt acgcccggga tgtccgtgac
      601 ggcaaaattc atgatccgca ctttctgccg gtgatttttg agcatcctcc tgaaatggtg
      661 gaaagcgggg ctcacctgct gatggaaaac ctcgccatgg ttaacccgaa tctcggttat
      721 tcggtggatg aggcctttct gtaccgggag tatcgtaaag cccgggaagc cggggaagag
      781 actttccgcg gtttcatgtc aaaacacgcc aatgtggaaa ttggtcttgc cctgcgttct
      841 gaccgctggg caggggcgga tttctgggag cagcagggca ggcgcgtcag cctggacgat
      901 atcctgcagc gcgctgatgt ggtgacggtg gggattgacg gcgggggcct ggatgatctg
      961 ctgggaatgt acgtgattgg ccgtgacagg gaaacccgcg aatggctggg ctggggccat
     1021 gcctgggtgc atgaaaccgc ggtggtcaga cggaagagtg agg
//
LOCUS       DAZFKM010000445         1054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFKM010000445 DAZFKM010000000
VERSION     DAZFKM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..213
                     /locus_tag="ACHKAY_005721"
     CDS             <1..213
                     /locus_tag="ACHKAY_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO5704133.1"
                     /translation="APKAKTPDYRAMDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVG
                     SRRISQDISTRDEGGGGKVVVIGRQR"
     gene            462..>1054
                     /locus_tag="ACHKAY_005722"
     CDS             462..>1054
                     /locus_tag="ACHKAY_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704134.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTVVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQYLASVILENLPERPFNIRMVRETADSTSDQLQNKTL"
BASE COUNT          279 a          252 c          330 g          193 t
ORIGIN      
        1 gcaccgaagg caaaaacacc ggattaccgc gcaatggata acggcagaca gaacacgtac
       61 ttttcctcac tggacaacat gattgctcag gggaacccga tgccggtgcc ttacggtgaa
      121 atgctggttg gttcacgacg gatatcccag gacatcagca cccgtgatga gggcgggggc
      181 ggaaaggtcg tggttatcgg gcggcagagg taaaaagaat aaaaaaatcc cgcagtgtat
      241 ggaggctgcg ggaacagaaa atgaagatta accacaggga gttttgtttt tattggcccg
      301 aaaaaactgt aacgcccggg aatgatatct gccacggggg cgtacagaaa atgtgaagaa
      361 attcagaaat tttattccgt catgacacag gcaccctccg gggtgcctgt cgtttttggg
      421 gcataaacag attcagacat cagacaggag agggggacag agtgggtaaa ggtggcggca
      481 aggcgcacac gccggtagag gcaaaggaca atcttaagtc cacgcagatg atgagcgtga
      541 ttgacgccat tggtgaaggg ccgattgaag gtccggtgaa ggggttgcag agtatcctgg
      601 tgaacaaaac cccgctgacg gacacggacg gtaatcctgt gatacatggt gtgaccgtgg
      661 tctggcgcgc cggggagcag gagcagacac cgccggaagg ctttgagtcc tccggggcgg
      721 aaaccgcact gggcgtggaa gtgacgaagg caaagccggt gacgcgcacc attacatccg
      781 cgaacattga ccgcctgcgg gtcaccttcg gggtgcagtc actgttggag accacctcaa
      841 agggcgaccg taatccctct tctgtccgac tgctgattca gttgcagcgt aacggtaact
      901 gggtgacgga aaaggatgtc accattaacg gcaagaccac ctcacagtac ctggcgtcgg
      961 tgattctgga gaatctgcct gagcggccct ttaacatccg gatggtccgg gagacagcgg
     1021 acagcacctc ggaccagctg cagaataaga cgct
//
LOCUS       DAZFKM010000446         1034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFKM010000446 DAZFKM010000000
VERSION     DAZFKM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..911)
                     /locus_tag="ACHKAY_005723"
                     /pseudo
     CDS             complement(1..911)
                     /locus_tag="ACHKAY_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          292 a          222 c          181 g          339 t
ORIGIN      
        1 tgccagtggt ttcggattgc tgtggcgaac ttctctgcgg ttaaatcagc agaactgata
       61 taatatctga ccgtcatttc tggctctttc ttttgttctg ctattatgga ccgaaaggag
      121 actgccacgc ataatttctt cagccctttc cattcaaacg tgaaatcaat aagttcatca
      181 gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactcatt
      241 gcgtaactgt catgctctgg attatttaat tctttcagcg gaaatttttc ctcaaaggct
      301 ttatttagcc gcccctggtt tccttttacc gcgaataaat aatcacctcc ctgtttttgt
      361 atcttttctg caatatcttt ctggcagccc atcgcatcag ttgtgatgat ttttccttta
      421 atatccatca tgttaagaag ttcaggaatg gctgtaatct cattggattt ctcatccgtc
      481 ttgatctgtc cgaggaccag actatgcatt gttgagaacg cactaatgac atgaatcgct
      541 cccctgcgac gactcttgtc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg
      601 tctttatcat ctgaagaatg gcagtcacgc atccagttaa taaagcactc gtgaaatttt
      661 gcaggactga tacaggatac aactctggca atgatatcgt gaacaggaat accattttca
      721 aaatcaccat atcacttcaa aacatcggga tgtgtttccc caaaatcctc tatatcttcc
      781 caaccttctg caccagaaat aacggcacaa atagtcaaca gtagaatatc cgataattta
      841 tgttccactt tccaggtttg tctgtaatcg gggataatag aaatatgttc catcaatttt
      901 ttaagttcca ttttgttctc cttaattagg taaggagtat ttgatcatgt ataagcaata
      961 aaaaacagct tcaggtaata aggaatatct caatttttaa acataaaatg cgaattattt
     1021 agtgcaaaaa gtga
//
LOCUS       DAZFKM010000447         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFKM010000447 DAZFKM010000000
VERSION     DAZFKM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1026
                     /locus_tag="ACHKAY_005724"
     CDS             <1..>1026
                     /locus_tag="ACHKAY_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO5704135.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTT
                     AQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRD
                     SVNQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPD
                     CGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETAD
                     HSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLETAGG"
BASE COUNT          344 a          248 c          261 g          173 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg ggcaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggaaa aagcactagc cttgggagct acggaaatca caaaagagga
      241 atacttcgca cgtaaaaacg gcacgtactc aggttcacaa caaaatactg gtgcatctga
      301 cacgaccgca caaccagagc cggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccaat atcagccagc ctgacgccga taagttgctt gctgcctctc gcggagaatt
      421 tgttgcaggg attagcgacc cgaatgatcc gaaatgggta aaggggattg aaacccgcga
      481 ttctgtaaac cagaaccagc aagaatcgga acagaacgac cagaaagcgg aacaaaacag
      541 cccaaatacg caacaaaacg agccagaaac gaaacaacct gagccagtag cgcaacagga
      601 accggaaaaa gtctgcaccg cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg
      661 cgcggtgatg ggcgacgcaa cataccagga aacattcgat gaagagtatc aggttgaagt
      721 tcaggaagat gatccggagg aaatggaagg cgctgaacat ccacacaagg agaacactga
      781 cggcaatcag catcacgata gcgataatga aactggcgag acggcagatc actcaattaa
      841 ggtgaacggt catcaagaaa tcacatccac cagcaggacg tgtgaccatc taatgatcga
      901 ccttgaaacc atgggaaaaa atcctgatgc cccgatcatc tcaataggtg caatattttt
      961 cgatccgcaa accggagata tggggccgga atttagtaag actatcgatc tggaaactgc
     1021 tggcgg
//
LOCUS       DAZFKM010000448         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFKM010000448 DAZFKM010000000
VERSION     DAZFKM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..500)
                     /locus_tag="ACHKAY_005725"
     CDS             complement(<1..500)
                     /locus_tag="ACHKAY_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5704136.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSAL"
     gene            complement(500..>1018)
                     /locus_tag="ACHKAY_005726"
     CDS             complement(500..>1018)
                     /locus_tag="ACHKAY_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFO5704137.1"
                     /translation="WHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIR
                     RNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIR
                     AKLDSLVTAGKLIGASCWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDE
                     FFGPAFAVLGGA"
BASE COUNT          236 a          296 c          245 g          241 t
ORIGIN      
        1 agcgcggaac ggatgcgatc gggcacaatg tccttgccgt ccttccggta aatgaagttc
       61 agcaggtcaa tctcccacaa cgggcgatcg ttaacactca gcttgtagta ggtgttttta
      121 atggcgtaag tgtgtgatgt ggcttcgccc tgtttggctt cccccatatc aatttccgtc
      181 acacgtccgc gcatttcgac ttcatacagg tcgctttctg catcggtgta atattcaccc
      241 gcaaaacgca gcagcgtgcc gtcaatcgtg ccgccatact taaggaacag ctcacgaact
      301 gcgcccccca tgacaaagct cgcatcaagc gcggagtcgt ccagaccgag atcaatactt
      361 accgcaccca tcatgccacc accccggtag ctgtcggttt tgcgcgtcag cttaggcaga
      421 gtgacggacg tcaccttacc cacttcgttt tcaccatcca caaacagcgt aaaaaagcga
      481 agatgttttg gcacagccat caggcacctc ccagcaccgc aaatgcggga ccaaagaatt
      541 catcagtaaa cgtctggtaa agctccatgt cttccagtgg cggaacgggc gtatatttgt
      601 agcgaatacg cacacgcccc tgacgtaaat ccgtggtgcc gttatccacc acgtcatacc
      661 agcacgacgc cccaatcagt ttcccggcag taaccagtga atccagtttt gccctgatgg
      721 cactgataac atctttcacg ttcgcaggcg tcagtggact gtcgatggtt tcaaactgcg
      781 cttccgcaat tgaatcagcc agcacctgtg cggttcgggt atacacctca aagatgtagg
      841 cgttcgtttc cggtgtgcgg ttgccccaga agcggaaccc gttgcgacga ataatggtcg
      901 tgatttcttt gttgttgagg ctgttggcat cactgtcttc ggcctgcaac gaccagaaca
      961 catgcctcga catccccagc acattcttaa ccggaacgtt ggagagtgat ttgtgcca
//
LOCUS       DAZFKM010000449         1018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFKM010000449 DAZFKM010000000
VERSION     DAZFKM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..500)
                     /locus_tag="ACHKAY_005727"
     CDS             complement(<1..500)
                     /locus_tag="ACHKAY_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO5704138.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSAL"
     gene            complement(500..>1018)
                     /locus_tag="ACHKAY_005728"
     CDS             complement(500..>1018)
                     /locus_tag="ACHKAY_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFO5704139.1"
                     /translation="WHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIR
                     RNGFRFWGNRTPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIR
                     AKLDSLVTAGKLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDE
                     FFGPAFAVLGGA"
BASE COUNT          231 a          295 c          248 g          244 t
ORIGIN      
        1 agcgcggaac ggatgcgatc gggcacaatg tccttgccgt ccttccggta aatgaagttc
       61 agcaggtcaa tctcccacaa cgggcgatcg ttaacgctca gcttgtagta ggtgttttta
      121 atggcgtaag tgtgtgatgt ggcttcgccc tgtttggctt cccccatatc aatttccgtc
      181 acacgtccgc gcatttcgac ttcatacagg tcgctttctg catcggtgta gtattcaccc
      241 gcaaaacgca gcagcgtgcc gtcaatcgtg ccgccatact taaggaacag ctcacgaact
      301 gcgcccccca tgacaaagct cgcatcaagc gcggagtcgt ccagaccgag atcaatactt
      361 actgccccca tcatgccacc accccggtag ctgtcggttt tgcgcgtcag cttaggcaga
      421 gtgacggatg tcactttacc cacttcgttt tcaccatcca caaacaacgt aaaaaagcga
      481 agatgttttg gtacagccat caggcacctc ccagcaccgc aaatgcggga ccaaagaatt
      541 catcagtaaa cgtctggtaa agctccatgt cttccagcgg gggaacgggc gtatatttgt
      601 agcgaatacg cacacgcccc tgacgtaaat ccgtggtgcc gttatccacc acgtcatacc
      661 agcactccgc gccaatcagt ttcccggcag taaccagcga atccagtttt gccctgatgg
      721 cgctgataac atccttcacg ttcgcaggcg tcagtggact gtcgatggtt tcaaactgcg
      781 cttccgcaat tgaatcagcc agcacctgtg cggttcgggt atacacctca aagatgtagg
      841 cgttcgtttc cggtgtgcgg ttgccccaga agcggaaccc gttgcgacga ataatggtcg
      901 tgatttcttt gttgttgagg ctgttggcat cgctgtcttc ggcctgcaac gaccagaaca
      961 catgcctcga catccccagc acatttttaa ccggaacgtt ggacagtgat ttgtgcca
//
LOCUS       DAZFKM010000450         1015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFKM010000450 DAZFKM010000000
VERSION     DAZFKM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..343
                     /locus_tag="ACHKAY_005729"
     CDS             <1..343
                     /locus_tag="ACHKAY_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO5704140.1"
                     /translation="TKAYFELGMTFPELYDDSHPEALARNTQKIFRWLDKDTPDAVEK
                     MQALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVASAVVLYDQMNR
                     GGPAGNAVVMH"
     gene            366..>1015
                     /locus_tag="ACHKAY_005730"
     CDS             366..>1015
                     /locus_tag="ACHKAY_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5704141.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGPMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGR"
BASE COUNT          260 a          229 c          299 g          227 t
ORIGIN      
        1 taccaaagcg tatttcgagc tgggaatgac gttcccggaa ctgtatgacg acagccatcc
       61 ggaagccctg gcccgtaata cccagaaaat tttccgttgg ctggataaag acacccctga
      121 tgctgttgaa aaaatgcagg ctctgttacc ggcgatcgaa aaggcgatgc cgcctttgct
      181 ggtggcccgt atgcgcagcc acagttctga atattaccgt gagatcgtcg aacggaggga
      241 tcggctggtg aaggatgtcg atgattttgt tgcgtcagcg gttgttttgt atgaccagat
      301 gaatcgcggc ggcccggcag ggaatgctgt ggtgatgcac taaaagcacg gtgttcgggg
      361 gttttatgag cagcaagctt catggtcttg tctgggaagg gtgcgccttc accggcatga
      421 tcttatccag ggtggcggtt atggcccgtc ttgcagacta cagcaatgac gagggcgtgt
      481 catggcctgc cattgaaact atccggcgtc agatcggtgc aagaagtgaa tccacagtga
      541 aatcggctat tgcagaactg gcgaaagagg gctggctgac gaaggaagag cgtaaggtcg
      601 gtgggcgtaa tgtaagcaat atctatcggc ttaatgtgga aaaactcgaa gcagctgcgg
      661 cggcggcgcg tgagtcatat aaaccgaaaa gaaaaattag cccggcaaaa aatgacccgt
      721 taacagttga cccgtcaaat attgacccct caacggttga cccgtcaaat tttgatggat
      781 caactgttga taaaaaactg ccgattaggg ggccgatgat tgaccccgat ccgtcagtat
      841 taaaacctga tccgtcagat aaaagatctt cttgtccgga cgcttcgcaa ccggacccgc
      901 agacggctga acaggatttt ttaacccgac accctgacgc ggttgtgttc agtgcgaaaa
      961 aacgccagtg gggaagtcag gaagatttgg tgtgcgcaca gtggatctgg ggacg
//
LOCUS       DAZFKM010000451         1010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFKM010000451 DAZFKM010000000
VERSION     DAZFKM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..795
                     /locus_tag="ACHKAY_005731"
     CDS             <1..795
                     /locus_tag="ACHKAY_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HFO5704142.1"
                     /translation="YLDSLDERGLILSLAAFSEDALGKMLLTFMLDNKASKELIEGFN
                     APLGTFSSRIKACFSLGLITEGQYKDLELLRKIRNKFSHSWENISIEDQDISQQIKAL
                     SFSRIDFECPKDNYQKIKKSISCLLIEIKITTSQIKKKHLKARLVGSNVNIGFSGKYE
                     EQVNDIKKNIESIKNDLTSHDKNIKSFAVHTANLLIERLSYVQFNHDDLDVFSDQLVD
                     ILEIKYQLLNLLGINGVTDLSQKEKEKLKKSFIERITIQTSNVSKK"
BASE COUNT          378 a          168 c          158 g          306 t
ORIGIN      
        1 tatttggaca gcctagatga aagaggccta atattgtctc tagcggcttt ctctgaagac
       61 gccttaggta aaatgctttt gacgttcatg ctcgataaca aagcgtctaa agaattaatc
      121 gaagggttta atgcccccct tggaacattt tcttcacgaa taaaagcctg tttttccttg
      181 ggactaataa cagagggtca atacaaagat cttgaacttc ttagaaaaat aagaaataaa
      241 ttctctcaca gctgggaaaa catttcaatt gaggatcagg atatttctca gcaaattaaa
      301 gcgttaagct tcagtcgtat tgattttgaa tgcccaaagg ataactatca aaaaataaaa
      361 aaatccatat cgtgtttact aattgaaatt aagataacca catctcaaat caaaaagaaa
      421 cacctcaaag caaggttagt tggttcaaat gttaacatag gattctccgg taagtatgag
      481 gaacaagtta atgatattaa gaagaacata gaatccataa aaaatgattt aacttcacat
      541 gataaaaata taaaatcatt tgcagtacat actgctaatt tactcattga acgtttatca
      601 tatgtgcaat ttaaccatga tgacctagat gttttttctg accaattagt agatatccta
      661 gaaataaaat accaattgct taatttatta ggaattaatg gagttacaga tttaagtcaa
      721 aaggaaaagg aaaagttgaa aaaaagtttt atagaaagaa ttacgatcca gactagtaat
      781 gtatctaaaa aatgataaaa ccacaccgac taaaactgta atgaacagag ttagtcggct
      841 ggtttctata acaaaagcca taccgtttga acactttttg ttttttcact atcatctcag
      901 aatggccatt gcacctcgct ctaaaataat gccccttggt tgaggttttc ccgcatttta
      961 aaagagcatt tcaatttatt attatataat tctctcacac aattaccaaa
//
LOCUS       DAZFKM010000452         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFKM010000452 DAZFKM010000000
VERSION     DAZFKM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..386)
                     /locus_tag="ACHKAY_005732"
     CDS             complement(<1..386)
                     /locus_tag="ACHKAY_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO5704143.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREA"
     gene            complement(386..>1002)
                     /locus_tag="ACHKAY_005733"
     CDS             complement(386..>1002)
                     /locus_tag="ACHKAY_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704144.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          241 a          253 c          232 g          276 t
ORIGIN      
        1 gcctctcgga tccggaacag tttttctgct tctgcatctt tcacccaggc tgtgccgttc
       61 cacttctgaa actccccttc cggcgataac caggtaacat tttccggtaa cggaccgagt
      121 tcagaaataa ataacgcgtc ccctgacgct acgtcataaa ccgttttacc ccgatggtct
      181 tcaacgagat gccacgatgc cttatcactg ttgaaaacag ccacaaagcc agcaggaata
      241 tctggtggtg caatatcggt actgtttgct ggcagacctg tatgaggcgg aatatatgcg
      301 tcaccttcac caataaattc attagttccg gccagcagat tataaatttt tatggtccgt
      361 ggttgttcac tcattctgaa tgccattatg caagcctcac aatatagtta aatgcgatgt
      421 ttttgacggt gttttccgcg ttaccagcag cgttaacggt gatggtgtgt ccatgtgaac
      481 caatcgcaac ggagtgcgta tgagcaccaa taccgacagt atgcgcgtgt gcacctgcag
      541 atgctgctgt gccggacagt gaatggctat gattaccatc tgtactggta ttcgctaacc
      601 accccgtaga catacctact gagccttgta caccccaggt attttgacct gagcttgtat
      661 aaccatattg ataagtatct ttaaaaacac tggggttaaa tcgacggcca tctctatggc
      721 tgtgattacc agctgcattc gtgctgccac ttaaactatg ggtatgcgca ccagtgttat
      781 tcgtggattt agtgccgtaa tcaaacgacg atgtggtttt cgtccccaaa tccgtactgg
      841 atgcgctggc gctgtgggta tgcgatttaa tgccgtcctg ttcctgagac aatacggccc
      901 gaccactggc aggtttgccc ttaatcgtcc agccacgcat atcagggatc acgcctgacg
      961 gataagccgc tgcaagtttc gggtatgcag atttgtcaaa ag
//
LOCUS       DAZFKM010000453          996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFKM010000453 DAZFKM010000000
VERSION     DAZFKM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..732
                     /locus_tag="ACHKAY_005734"
     CDS             <1..732
                     /locus_tag="ACHKAY_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO5704145.1"
                     /translation="RFNIWCQGATPWMGNGAWAECAGTFTEEDLHGQECYAGLDLSST
                     SDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRAVYRQWAKAGWIRTTPGDCID
                     YDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAGFEVEPFPQTYLRFSPAAKSF
                     EVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNKKKSPNKIDPSVAALMAFGTF
                     QAEHEDFAFDISDSHRQKLEEFSGV"
     gene            746..>996
                     /locus_tag="ACHKAY_005735"
     CDS             746..>996
                     /locus_tag="ACHKAY_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704146.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITY"
BASE COUNT          252 a          234 c          294 g          216 t
ORIGIN      
        1 cgatttaata tctggtgcca gggggctacc ccgtggatgg gtaacggcgc atgggctgag
       61 tgtgccggaa cgttcacgga ggaagattta cacgggcagg agtgttacgc ggggctggac
      121 ttatcatcaa ccagcgatat ttccagcgtg tgctatgcct ttccggtcgg taaaaatatc
      181 atgctggttt cccgtcatta tctgccggag ttccagctac agaaccccgc caataaaaac
      241 cgcgctgtct atcgccagtg ggctaaagcg ggctggatac gcacaacgcc aggtgactgc
      301 attgattacg accggatcag agatgacatc atgcaggatg cggagaaatt taatatcagg
      361 ctggtgggct ttgatacgtg gaacgccacg catctcagga cgcaattaca gggggctggt
      421 tttgaggtgg agccgttccc gcaaacctac ctcagattca gtccggcggc gaaatcgttc
      481 gaagtttttg ttaaccgcag ggtgattgtg catcgtggcg atccggtgtt gtcctggtcg
      541 atgagtaacg tcgtgatgca gagtgacgcg aacgccaata tcaagccgaa caagaaaaaa
      601 tcaccgaaca agatagaccc gagcgtagcg gcgctgatgg cgtttggtac attccaggca
      661 gagcatgagg attttgcttt tgatataagc gacagccacc gccagaaact ggaagaattt
      721 agcggggtgt aatgaggtca gcagcatgac agaagccgaa atactgcgat taatccgccg
      781 cgtctctgga atcagccagc aggctgacga acagaccacg cagccggaca gcgtgacagc
      841 cgaaaattat gcgcgtgttg ttgctgaggt gatgcgccgt gatggtatcc agcttaatga
      901 tgcggatatg cgcgacatac ggatccgcgt tcttgaaatg ctggcctaca atcgccgcgt
      961 tgaactgtat cgggagaagg agaaaatcac gtacca
//
LOCUS       DAZFKM010000454          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFKM010000454 DAZFKM010000000
VERSION     DAZFKM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..848)
                     /locus_tag="ACHKAY_005736"
                     /pseudo
     CDS             complement(1..848)
                     /locus_tag="ACHKAY_005736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            825..995
                     /locus_tag="ACHKAY_005737"
                     /pseudo
     CDS             825..995
                     /locus_tag="ACHKAY_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          238 a          291 c          259 g          207 t
ORIGIN      
        1 ccacggcaca tctgcagcac tgccaccatg gcactgcgcg ttaaacacgg agaggtcagc
       61 acgaaactgt gtcagcatgg ctgtaaacag cgcaggttgc tgtacgtggg tggcggcgct
      121 catgtcaaac tcaccctgca tccagcagac ggccagcaga acgtttttgg gatttttctg
      181 caatgccgct tttgtgcggg aaatcagatc ctgatataac ggcttgccca ccccccagcg
      241 tgccgaatcc tgactggccc ccgtggactc gctgaatgtc ccctccgcgc cctgggtaaa
      301 tgccgaacca ccacgacagc atggtaccag caggatcccc gcgttattcg ggatatacgg
      361 gagcagtttt ttggcaatat gtaaaccctg gccgacacag ccgtactgcc ctttgctcag
      421 gtcagccctc ggatgattca gcgtactcat atcctgcaca tcatgcagac agtggtcagc
      481 cggaataata tcgttatatc tgcaggcagc cccgcccggc gtcactgtac tgcggcgcgc
      541 cagctgttta atgcgcggat ccggagcatc gtatgaatcc ggcagcggaa gcccttcacc
      601 gtaagccctt caccgtaagc catggcattg gattgcccgg ccagtacgat gacgtagtac
      661 caatccggct cagatgaagg gccgacctgt ggctctcctt caatagccac cgcctgcatc
      721 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      781 tgtgtcagct tttccgcaag gtctgacggc gacgccgccc tgacaacatc gtaatgttta
      841 atcgacatcg aatttctccc gtgtagagga acagagttaa aaagccggaa gcggaatcaa
      901 atcacaggat gaccatctgc cagtggcagg tcataaaaaa aaggctgcgc aatgcgcagc
      961 cagaactcac aaggaaaatg ataaaaggaa taaca
//
LOCUS       DAZFKM010000455          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFKM010000455 DAZFKM010000000
VERSION     DAZFKM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>995
                     /locus_tag="ACHKAY_005738"
     CDS             <1..>995
                     /locus_tag="ACHKAY_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704147.1"
                     /translation="YSVFTDKTHQIIKQKYFNDIVPMILALDIRNTYRPDNEMAFYYH
                     IHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRNIRKN
                     STPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLAFERRTS
                     LTSILATLYKMLISEREVSEYECQLLYLTNPIDVMNILNKYIYYFPNENSPFYTLKID
                     SALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIVFGGYIYTLNNGVTLQNIENS
                     LKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHRTINKLNYEKLPFGFIIAAFAIL"
BASE COUNT          361 a          177 c          150 g          307 t
ORIGIN      
        1 ctattctgtt tttacagaca aaacacatca aataataaaa caaaaatatt ttaatgacat
       61 tgtaccaatg attctcgcac tcgacatcag gaacacatat cgcccggata atgagatggc
      121 attttactat catattcatt cttttctcac tcagataccg gataatgagg atgatatata
      181 tcatgctgca aggacatatc tgcgaaatta cgttaagtta tgtttatccg gatacacgcc
      241 agcgaatgcg catttcaaag atatctttga tggcgtatat gaattcattc gtaatattcg
      301 caaaaacagt acaccaggaa aaacaaaact tatcgcaact atcaacacat gcaaagaaac
      361 ctgtaaacat ctgctttatt taagtaatga agacaaggaa aaaataattt ctgacttaga
      421 taaagttcag gttgcatgtt attatctcac tatattactg gctttcgaaa gacgaacttc
      481 attaacaagc atcctggcaa ctttatataa aatgctgata agcgaaagag aagtttcaga
      541 atatgaatgc cagttattat atttaaccaa cccaatagat gtaatgaata tactgaacaa
      601 atacatatat tactttccta atgagaactc accattttat acactgaaaa ttgacagtgc
      661 attatcgtgg gatgccattg acgcaatacg agactatagt atttctgata tttatcttta
      721 tcctgaacaa aaaacaataa attgtgtcgt tgagattgaa aacattgtct ttggcggtta
      781 catttataca ttgaacaacg gcgtcacatt acaaaacata gaaaactctt taaaagattc
      841 ttcatgccat tatgtcttaa atggctatac agaatttgtt aactgtttga gacaacttac
      901 ttcaggaaag actgaaagtg ttcatcgcac catcaataaa ctgaactatg agaaattacc
      961 ttttggattt atcattgccg cgtttgctat actaa
//
LOCUS       DAZFKM010000456          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFKM010000456 DAZFKM010000000
VERSION     DAZFKM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>987
                     /locus_tag="ACHKAY_005739"
     CDS             <1..>987
                     /locus_tag="ACHKAY_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YopT-type cysteine protease domain-containing
                     protein"
                     /protein_id="HFO5704148.1"
                     /translation="PSITTIIKKTEYHGHQLTNAMTVASVVKPYDFSNLGAINSIDKS
                     VSDIPALHTIVEQAKYNLLSWNDFYNTHASIWDTIARQHKSTNIEFHPQSLLFDRDSK
                     GKCLGLSLLYLDTGGYGSRYQKLRHNIETASALYQTKYNDNIKLSNSDDFFLRKTQRI
                     ITMSNELGNNRLKNSQLEILELKDPVLTEGILYQRRISSLLITTEYHSLALQQVSSFW
                     RVTDPNFGHCDFHSLAQALTFIKNITSNGNFSSLYGSGIIKIYFSESPNNWKYIKLPL
                     IQNNSLSRDIYLTTPEKLSATGDSLNIMGHLVPVSFIYDIGGVFNGNRISEST"
BASE COUNT          355 a          161 c          162 g          309 t
ORIGIN      
        1 cccagtataa caacaataat aaaaaaaaca gaatatcatg gacatcagtt aacaaatgct
       61 atgacagttg cttcagtggt taagccatat gactttagta atcttggtgc cataaatagt
      121 atagataagt ccgtttctga tatacctgca ttgcatacaa ttgttgaaca agcgaagtat
      181 aatttacttt catggaatga tttttataat actcatgcgt caatatggga tacaatagca
      241 aggcagcata aaagtacaaa tatcgaattt cacccacaaa gtcttctttt tgatagagat
      301 tcgaaaggaa agtgccttgg tttgtctctc ctgtatcttg atacaggagg ttatggaagc
      361 agatatcaaa aactgagaca caatattgaa actgccagcg cattgtatca aacaaaatat
      421 aatgacaata ttaaattatc taatagcgat gatttctttt taagaaaaac acaacgtatc
      481 attacaatgt ccaatgagtt aggcaataat agattaaaaa actcacagtt agaaatccta
      541 gaattgaagg accctgtact aactgaaggt atattatacc agcgtcgaat atcatctctt
      601 ttaataacaa cagaatatca ctcactcgcg ttacaacaag tatcttcttt ttggagagta
      661 accgatccta attttgggca ttgtgacttc cattccttag cacaggcttt aacgtttata
      721 aaaaatataa cgagcaatgg taacttttca tctttatatg gaagtggtat tattaaaatt
      781 tactttagtg agtctcctaa taattggaag tatataaaac tccctcttat tcagaataat
      841 tctttgtcaa gggatatata tttgacaact ccagagaaat tatccgcaac tggagattca
      901 ttaaatatta tgggacattt agtgcctgtc agttttattt atgacattgg aggagtcttt
      961 aatggcaaca gaataagtga aagcaca
//
LOCUS       DAZFKM010000457          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFKM010000457 DAZFKM010000000
VERSION     DAZFKM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..947
                     /locus_tag="ACHKAY_005740"
                     /pseudo
     CDS             1..947
                     /locus_tag="ACHKAY_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          284 a          225 c          252 g          208 t
ORIGIN      
        1 cgtaaggcgt taaatgaagc tcgccttgag cgagatatat taaaaaaagc aacagcgtat
       61 tttgcacagg agtcgctgaa aaatacgcgt taatcgaaca atggcgacaa caatttccca
      121 ttgaagcgat gtgtcaggta tttggtgtat ccaggagcgg ttattacaac tgggtacagc
      181 atgaaccctc agacagaaaa caaagtgatg agcggctaaa actggagatt aaggtggcac
      241 atatccgcac tcgcgaaaca tatggaaccc ggcggctcca gacggagctg gcagagaatg
      301 gcatcatcgt tggtcgtgac cgactggcac gtcttcgtaa ggagctaagg ctacgctgta
      361 agcagaaacg caagttcaga gcgactacga actcgaacca caatctgcca gttgcgccaa
      421 atctgctgaa ccagacgttc gctcctacag caccaaatca ggtctgggtg gcggacctga
      481 cgtatgttgc cacacaggag ggatggttgt acctcgctgg catcaaagat gtttatacgt
      541 gcgaaattgt cggctacgcc atgggagagc gcatgacaaa agagctgaca ggtaaagctc
      601 tgtttatggc gctcaggagc cagcgcccac ctgccgggct aatccaccac tctgatcgag
      661 gttcacagta ctgcgcatac gattaccggg tcatacagga gcagtttggt ctgaaaacat
      721 caatgtcgcg taaaggtaac tgttacgaca acgctccgat ggaaagcttc tggggaacgc
      781 tgaaaaatga gagcctgagc cactatcgtt ttaataaccg ggatgaagcc atctcagtaa
      841 tacgggaata cattgagatt ttctacaatc gtcagcgtcg tcactctcgt ctggggaata
      901 tctccccggc agccttcagg gaaaaatatc atcagatggc tgcttaaaaa aagaacaaat
      961 ggtagtgtc
//
LOCUS       DAZFKM010000458          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFKM010000458 DAZFKM010000000
VERSION     DAZFKM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..882
                     /locus_tag="ACHKAY_005741"
     CDS             <1..882
                     /locus_tag="ACHKAY_005741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5704149.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          244 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaact
//
LOCUS       DAZFKM010000459          956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFKM010000459 DAZFKM010000000
VERSION     DAZFKM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.465"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            147..332
                     /locus_tag="ACHKAY_005742"
     CDS             147..332
                     /locus_tag="ACHKAY_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO5704150.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            573..>956
                     /gene="nleB"
                     /locus_tag="ACHKAY_005743"
     CDS             573..>956
                     /gene="nleB"
                     /locus_tag="ACHKAY_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO5704151.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYD"
BASE COUNT          331 a          161 c          175 g          289 t
ORIGIN      
        1 tgagtatcac aggcacatgt attttagccc cctgagtcta cagacaacca atattactta
       61 aataagtgat aatcttaatg ctgtttttta gaacagatga ttgatatttt tgggccggag
      121 aaacgcagac aggctttgtt gaataaatcg agcttttgct gagtttaagg atcagatcac
      181 tcattttcct gcaaaagctc attgggcgaa acaatctgac tcagagaacc cggattaaac
      241 gtctggcccg cagaaccatc tatttctcac gcactgtagt acgtaataaa aaagtcatcg
      301 gtacttttat caaaaaacat atattctatt aattattgcc atcacctttt tcttattgac
      361 aacattactc tgctgattgt taaaatctaa cctgtattca gggttacgat tctatacaat
      421 agaacaggga ggggaaagga tgtcatcgtt aatgagactt tttgttaatg tcaacaatgc
      481 tatgaattta ttttagaaaa acaattaagc tgacgtagtg ttccgtctaa atgacggggc
      541 gatattaaca tgattagaac aagaggaatt ttatgttatc ttcattaaat gtccttcaat
      601 ccagcttcag aggaaagaca gccttatcaa atagtacact tctccagaaa gtttcttttg
      661 ctggaaaaga atatcctctg gaacctattg atgaaaaaac ccctattctt tttcagtggt
      721 ttgaagcaag gccagagcga tacgaaaaag gagaagtacc aatattgaat accaaagaac
      781 atccgtattt gagcaatatt ataaatgctg caaaaataga aaatgagcgt ataatcggtg
      841 tgctggtaga tggaaatttt acttatgaac aaaaaaagga atttctcagt cttgaaaatg
      901 aatatcaaaa tataaaaata atctaccgag cagatgtgga tttcagcatg tatgat
//
LOCUS       DAZFKM010000460          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFKM010000460 DAZFKM010000000
VERSION     DAZFKM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..245)
                     /locus_tag="ACHKAY_005744"
     CDS             complement(<1..245)
                     /locus_tag="ACHKAY_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5704152.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..586)
                     /locus_tag="ACHKAY_005745"
     CDS             complement(245..586)
                     /locus_tag="ACHKAY_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704153.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(579..>947)
                     /locus_tag="ACHKAY_005746"
     CDS             complement(579..>947)
                     /locus_tag="ACHKAY_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704154.1"
                     /translation="AVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPE
                     AIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLI
                     DQRVQAVMLSMRRDGGMLSG"
BASE COUNT          195 a          341 c          210 g          201 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctggta cttccgcccc tgccaggtca ccggctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcacca ccctgggcct tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca ggctttcccc gtgaatatgc
      241 tgcatcagtt caccacctgc tcaatcgtac agctgaaatc actgtaccgg acgttatccg
      301 tgacgctcca ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca
      361 aaaaggcacg gtaaccacca tgccaggata aaaattcatc cagccagcgc cgggttgact
      421 catccgtcac ccggaacacc gcctgaaacg tcttcagttg aggattcagt cctgtggggc
      481 gacgctgttc ataaccgtca ccaaaccgta ccctcgccac cgacggtttc tcactcacct
      541 gcatcccttc acgcgggacc agatgcagcg tttttatctc agccactcag cattcctccg
      601 tcacgtcgca tggacaacat caccgcctgc acccgctggt caatcagctg cacaagactg
      661 cctgccgcct ccggccctat ctggccatta gtcccgtcat tctgaatggc gatatggtag
      721 accggggaat acaccaggcc cgcactaccg ttcatactgc ccaccgctcg cacacccagc
      781 gagccatccg ccgcccgggt caggggcata atggcttcag gaccggcctc ccccatcagt
      841 cccgcccctt ttgcaaaggc aaagtacgtg ggcgtatcca caatactgtt gctgtacgca
      901 ctcaggtttg ccgaggtata cacgccgcct tttgcattgg ccaccgc
//
LOCUS       DAZFKM010000461          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFKM010000461 DAZFKM010000000
VERSION     DAZFKM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..539)
                     /locus_tag="ACHKAY_005747"
     CDS             complement(<1..539)
                     /locus_tag="ACHKAY_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704155.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(795..>943)
                     /gene="tdcR"
                     /locus_tag="ACHKAY_005748"
     CDS             complement(795..>943)
                     /gene="tdcR"
                     /locus_tag="ACHKAY_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO5704156.1"
                     /translation="SRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCEC
                     NRFP"
BASE COUNT          294 a          170 c          178 g          301 t
ORIGIN      
        1 caataaaaat cattattaaa atgatcgggt gaaaaaaaga gaatttgcca acaatctgaa
       61 gtaatgctgg ttttattaaa gtgcacaagg ggtgttaaga tggcttcttt tcgggtgaat
      121 tttgcacaat acttagaaat cgtctcctta atacattcca gctctttttt attgacggct
      181 gagcaagtac attgttcgcc aaaaggtgca aaagtgccta catctaatgt ttgtatgata
      241 taatcatcat gttcccctgt agaaatatct atgatgaatg gactcatatt ttccggttgg
      301 attcggatca ttacagaagg aacatcgcct tgatagaaat tcaatgcagt aattgcagat
      361 aatactctcc gcagttcatt acttatactg taaattttat taacgtcatt ggtggagaca
      421 atttctgcaa actttctttt taccgtccaa taatcaatat ttctattgtg attgttaacc
      481 actgcgtgca ttggcgggat tgaagggtga aaaatatgtg caattgggaa gcctttcatt
      541 gatgcctgcc ttgtcgtcat gacaaacaat aatagatgct tattttcctc gcagggttct
      601 ttcgcactat caaaaaacgc tcatttaccc ggcgttacag taaacgaagc aacattctgt
      661 ggcctacgac gatcaaaagc tctgtgaaaa tttcccttgt tgtctttgtc tgaccttatc
      721 gcggcgacca gcaattattc gttttgtgat cctcaacaca aaatgctatt cgtcgcctat
      781 gaggggagaa gaatttatgg aaaacgattg cattcacaaa atgacgtcct gtcagaagag
      841 aaaaagttga cgttaatcaa ttgttcgaga tgatcaatac ctgtattcga aattttaaaa
      901 tattgataaa ataaatgttt gttctccaac aaatagcgtg aaa
//
LOCUS       DAZFKM010000462          934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFKM010000462 DAZFKM010000000
VERSION     DAZFKM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..372)
                     /gene="ydcD"
                     /locus_tag="ACHKAY_005749"
                     /pseudo
     CDS             complement(1..372)
                     /gene="ydcD"
                     /locus_tag="ACHKAY_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
     gene            complement(356..>934)
                     /locus_tag="ACHKAY_005750"
     CDS             complement(356..>934)
                     /locus_tag="ACHKAY_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO5704157.1"
                     /translation="NRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLD
                     AIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLM
                     CRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACI
                     EKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          280 a          211 c          140 g          303 t
ORIGIN      
        1 ttttatcact aaaatacacc cgttgttttg tagcgcagaa ctccatattc tccagtttac
       61 taatgttttt taatcgcacg ctataaagaa tatcattgaa aaaattaaga tttatatcaa
      121 ccactccaca gtgcgtcctg taattattaa ccattatact tataaaatga ccattccctc
      181 tactaatttt ttggtcatta attattttat aaataatatt ttttcagaaa gatatatctc
      241 aacgctcttt ggtgttttat gtccataaaa tacgatgtca gaatttgaat tgttatcaaa
      301 cccaaggtaa acaacaaata aacaaagggt aattaaaaaa atgacaagtt tgcctctaat
      361 aatttgaagc atctgaactc ccttgctttg cttttattgg attatctgct tttataccat
      421 ctaacccgaa gagttgccca gtgttatatt tttcaataca tgcatcgcta catttggcag
      481 aaaaatcgcc acaagcaata ccgacagtgt tatttttaag atccatgatt ctttctttag
      541 ctggctgacc ttgtctatcc cctgccgcct catgattttt acctatcacg tcagctattg
      601 ttgatccaaa tgtcttagtc attcgacaca tcaagtaaca atgtctcatt gcatcggcgg
      661 ccccattatg agctccgcca acaatagcat ccatatcata accgaactgg taagcactgt
      721 tagtaatgct gtttgcagca atcaatccag gtacaccaga tacggcataa attagtccac
      781 ctgatgtcat attctcaatc gcatctaacc ccattgggtc tatcacttgt atgggattca
      841 acggatactg atacatattc catccacctc tcaacccaat cgggtccggg gtgatatacc
      901 gcccctgcaa cggatcgtag taccggtgac ggtt
//
LOCUS       DAZFKM010000463          933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFKM010000463 DAZFKM010000000
VERSION     DAZFKM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.469"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            77..862
                     /locus_tag="ACHKAY_005751"
     CDS             77..862
                     /locus_tag="ACHKAY_005751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704158.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          306 a          186 c          201 g          240 t
ORIGIN      
        1 attcaagtgg agtatataat taaaaatcgt tagctaaaca ttaccatgat tatgacaggc
       61 gagggagtgt ccaattatga attcaaaaaa gctttgttgc atatgtgtgt tattctcgct
      121 gcttgcagga tgtgcctctg aatcttctat tgatgaaaag aagaaaaaag ctcaagtcac
      181 acaaagtaat attaataaaa acactcccca gcaactgaca gacaaagatt tattcggtaa
      241 tgaaaccact ctggccgtat ccgaagagga tattcaagct gcgcttgatg gagatgagtt
      301 ccgcgttccc cttaattctc ctgtaatact tgttcaatcc ggcaaccgcg caccggaaac
      361 cattatgcag gaggagatgc gaaaatatta tactgtttcc acattctctg gtatcccgga
      421 caggcaaaag cctctgactt gtaacaaaaa caaagataaa aatgagaacg aggatgttgc
      481 cagtgctgag aatatgaact ggatgcaggc actgcgtttt gtggctgcaa aagggcatca
      541 gaaagcgatt attgttttcc aggatacgtt gcagacagga aaatatgact ctgcgctgaa
      601 atcaacagta tggtccgact ataaaaatga aaaactcacc gacgctatct ccctgcgcta
      661 cctggtacgt ttcacgctgg tggatgtggc aacaggtgag tgggctacct ggtcgccggt
      721 gaattatgaa tataaagtgc ttccaccatt gcccgacaag aatgaagcca gtacgactga
      781 tatgacagag cagcaaatca tgcaacttaa gcaaaagacc tataaagcga tggtaaaaga
      841 tttggttaac cgctatcaat aataaattat atctgccgcc aggaatttct ggcggcaata
      901 atacaaaatt ctttgcataa gaaatttttc ttg
//
LOCUS       DAZFKM010000464          933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFKM010000464 DAZFKM010000000
VERSION     DAZFKM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            62..880
                     /locus_tag="ACHKAY_005752"
     CDS             62..880
                     /locus_tag="ACHKAY_005752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO5704159.1"
                     /translation="MKRILFIAFFIAMFVTPIALILFVFDYGYKKSPQEYSLSFIKQL
                     PNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARSGDIIGRAPLPFISDAETIEHIQGN
                     IFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFEGLAWKAEGRMLFVAKE
                     RRPTGMFAYQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNNESLMILSDESRKLLKF
                     NLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          311 a          182 c          170 g          270 t
ORIGIN      
        1 cagcaatcat acaaaacaaa gatatttatt tcgcaaacca tttatagcga atggagaagg
       61 aatgaaaaga atcttattta ttgcattttt tattgcaatg tttgtcacac caattgcatt
      121 aatattattt gtatttgatt atggttacaa aaaatcacca caggaatact cactttcatt
      181 tataaaacaa ttaccaaata cggtgaactt atcatctctt acgtataata aagaggatga
      241 tttcctgtat gccacccaga atagccctgc gcagctactt aaaatagcca gaagtggcga
      301 catcatcggc cgagccccgc tgccttttat ctctgatgcc gaaaccattg agcacataca
      361 agggaacatt tttgctgcag tagacgaaaa gacttctgaa ctattctttt ttacagttac
      421 aaaagatatg catatctcct tgcgaaataa aattcagtta gaaaaattca ataaaaaaaa
      481 tagagggttt gaaggactgg catggaaagc agaaggccgg atgctgtttg tcgctaaaga
      541 acgacgccct acaggcatgt ttgcctatca gctttctcca gatctgctga gtgcaaaaca
      601 agtaacgatt ccagaggaac taaatgatat acatgttaaa gatatatccg gcctggactt
      661 taataatgaa agtctgatga ttctgtccga tgaatcgcgt aagttactca agtttaacct
      721 gacagaaatg tctttcgttg agatgatgga cctgacaaaa ggaaatcatt ctctgaccag
      781 tgatttgctc caacctgaag gcattgtaac tctgtctgat gaaagcattt atgtcgctag
      841 tgaacctgat atcctcgcta aatttgtccc taataaataa gtctgtcact agccttagta
      901 ttgagcgacc aaatctggca atctcatgaa act
//
LOCUS       DAZFKM010000465          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFKM010000465 DAZFKM010000000
VERSION     DAZFKM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /locus_tag="ACHKAY_005753"
     CDS             <1..117
                     /locus_tag="ACHKAY_005753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HFO5704160.1"
                     /translation="RRIEYTAASLDDLNRAINDAESVLGTTSRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHKAY_005754"
     CDS             114..>931
                     /locus_tag="ACHKAY_005754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704161.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          191 a          216 c          322 g          202 t
ORIGIN      
        1 cgaagaattg aatatacggc ggcctctctg gatgatctta accgggcgat caatgatgcg
       61 gagtcagtgc tggggacaac aagccgccgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggtg ttccgcttcg ggagagcctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gttgcccgcg ttgcgtctgg gcaatgcccg tgcagatgat ctggtgcgca
      301 ataacgggat agcggccaat gcggtggcac tgcataagga tcatattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagact gcagcaaaaa
      421 gttttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga agggaaacgc acgtttacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggagagcacg caactgtttc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctctggta gtggacgatg ggaacggatc ccgcgtgaac
      781 tacccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggctaa tcagttttac agcgtcatgg aacggctgaa gatgctggat tccctgcagg
      901 caacacagct tcagtcggcc attgtgaaag c
//
LOCUS       DAZFKM010000466          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFKM010000466 DAZFKM010000000
VERSION     DAZFKM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /gene="gpW"
                     /locus_tag="ACHKAY_005755"
     CDS             <1..117
                     /gene="gpW"
                     /locus_tag="ACHKAY_005755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO5704162.1"
                     /translation="RRIEYTAASLDELNRAINDAESVLGTTRRRRRPLGVRL"
     gene            114..>931
                     /locus_tag="ACHKAY_005756"
     CDS             114..>931
                     /locus_tag="ACHKAY_005756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704163.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKA"
BASE COUNT          189 a          224 c          321 g          197 t
ORIGIN      
        1 cgcagaattg aatatacggc ggcttctctg gatgagctta accgggcgat caatgatgcg
       61 gagtcggtac tggggacaac ccggcgtcgc cgtcgtccgc tgggagtgag gttatgaaac
      121 gaacgcctgt cctgattgat gtgaacggcg ttccgcttcg tgagagtctc agctacaacg
      181 ggggcggtgc aggatttggc gggcaaatgg ctgagtggtt gccaccggcg cagagtgccg
      241 atgcggccct gctgcccgcg ttgcgtctgg ggaatgcccg ggcagatgat ctggtgcgca
      301 ataacggaat agcggctaat gcggtggctc tgcataagga tcacattgtc gggcatatgt
      361 ttctgatcag ctaccgtccg aactggcgct ggctggggat gcgggagacc gcagcaaaaa
      421 gctttgtcga tgaggtggag gcggcctggt cggaatacgc cgaagggatg tctggcgaga
      481 tcgacgtgga aggaaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg
      541 cgtttaacgg cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc
      601 gtacgcgttt taaagccgtg agtccgaaac gggtggacac gccaggacac ggtatgggga
      661 accgttttct gcgggccggg gtggaggtcg atcgatatgg ccgtgccgtt gcgtaccata
      721 tctgtgagga tgattttccg ttctccggga gtggacgatg ggaacggatc ccgcgtgaac
      781 ttcccaccgg gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc
      841 gtggggccaa ccagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg
      901 caacacagct tcagtcggcc atagtgaagg c
//
LOCUS       DAZFKM010000467          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFKM010000467 DAZFKM010000000
VERSION     DAZFKM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..376
                     /locus_tag="ACHKAY_005757"
     CDS             143..376
                     /locus_tag="ACHKAY_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704164.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            382..681
                     /locus_tag="ACHKAY_005758"
     CDS             382..681
                     /locus_tag="ACHKAY_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO5704165.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            678..>931
                     /locus_tag="ACHKAY_005759"
     CDS             678..>931
                     /locus_tag="ACHKAY_005759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO5704166.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNI"
BASE COUNT          281 a          200 c          235 g          215 t
ORIGIN      
        1 tctgaaatta tgcgggtggc ttttttcttt ctttgcactg atgagcacag agatacaggg
       61 ctaaatattc ttgatattgc cgctaactat gctgatttcg tgacagaagc tgttttaaga
      121 aaaacaacgg acggggatta atatgcgtga tatttaccac gaaacaatag accgcgcatt
      181 tagtgccctt gcttacgctg aaggtatgta cgaaatattg cgcatatggc ttgaaacact
      241 tggcgacaat gaacgcgaca aacaaaaatc aagaattgtc acggcattaa taacgcttct
      301 tgagcctgta ataaatgagt tgcaggaaat agagacattg cacgacagat ataacgaaca
      361 gcacaccgga gaataaaaat aatgaaactt aaatattctg gcttaactgc cagtggcaac
      421 actcacccta aatttacgcg cggtgatatt taccgcgacc agtacggcgg cacggtaatg
      481 attaagggcg tggcgggacg gtgcgtaact taccgccgtg aaggttacga atatgattgc
      541 gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg tacagaccgc gccgcataac
      601 gtgcccacca gcaacgccag ggcacgggca aacatccaga agctgaaaac catgattaac
      661 ggattcaggg gcaagaaatg aaaagcgcac cgaacttaaa aaaacagcct tacgacaaga
      721 tgaccgaagt cattattttt gcgggtagtg atgcctgggc acatgcgaaa cagtggcagg
      781 aacaggacgg gcgactggct ggcgataatg tgcctcccgt tgtgctggct gatgatcaac
      841 tggatgaact ggcagacctg agaatcatcg acgaggggcg ctattgtgtc cggctgtaca
      901 aggcaggcca catcaggcca tcaaatatta a
//
LOCUS       DAZFKM010000468          924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFKM010000468 DAZFKM010000000
VERSION     DAZFKM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..659
                     /locus_tag="ACHKAY_005760"
     CDS             <1..659
                     /locus_tag="ACHKAY_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO5704167.1"
                     /translation="EEWREWQLAEGRKRSEEINRLNHQVRVEKILNRAGIQPLHRKCS
                     FGNYRVQNDGQRHALSQAKSIADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGR
                     SVIIVTVSDVMSVLHDGYDNGQSGEKFLQELCGVDLLVLDEIGMQRDTRNEQVTLNQI
                     VDRRTASMRSVGMLTNLNHAAMSTLLGDRVMDRMTMNGGRWVNFNWESWRSNVGRQGM
                     "
     gene            685..>924
                     /locus_tag="ACHKAY_005761"
     CDS             685..>924
                     /locus_tag="ACHKAY_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704168.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAP"
BASE COUNT          243 a          197 c          290 g          194 t
ORIGIN      
        1 tggaggagtg gcgggaatgg caacttgctg aaggacgaaa acgcagcgag gagattaacc
       61 gcctgaatca tcaggtgcgg gttgaaaaaa tcctgaaccg tgcgggcatc cagccgcttc
      121 acaggaagtg ctcattcggg aactaccggg tgcagaacga cggtcagcgc catgctctga
      181 gccaggcgaa atccattgcc gatgaattga tgaccggatg tacaaacttc gtgttcagcg
      241 gtaaacctgg taccggtaaa aatcacctgg cagcagcgat tggcaatcgg ctgatggcga
      301 aggggagaag cgtgattatc gtcaccgtgt ccgatgtcat gagcgtgttg catgacggct
      361 acgacaacgg ccagtccggg gaaaaatttt tacaggagct ttgtggagtt gaccttctgg
      421 tccttgatga aattggcatg cagcgggata cgcgcaacga gcaggtcacg ctgaaccaga
      481 tagtcgaccg cagaacggct tcgatgcgta gtgtcggaat gctgacgaac ctgaatcacg
      541 cagcgatgag cacactcctc ggagatcggg tgatggaccg tatgaccatg aatggtggtc
      601 gttgggtgaa ttttaactgg gagagctggc ggtcaaacgt tggacgtcag ggtatgtgag
      661 aatttttgac gaggtaaatt ttcgatggaa actgtattgc atgcactgaa agcgatggga
      721 aaagccaatt ctgttgaact ggcggcgcgg cttgatatca gccgtgaaga agttcttaac
      781 gaactgtggg aactcaaaaa aaatggcgtt gttgataaaa cgggtcacac ctggtttctg
      841 gctgtcgaag gtgaagccgg ggtaaccgaa gggcaggcac tacaacctga agcgccggat
      901 gtggtaaccg aagaggtcgc tcca
//
LOCUS       DAZFKM010000469          915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFKM010000469 DAZFKM010000000
VERSION     DAZFKM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..490
                     /locus_tag="ACHKAY_005762"
     CDS             <1..490
                     /locus_tag="ACHKAY_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704169.1"
                     /translation="GEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVSSIGKAVT
                     AKEVITRTVKVTNVGKPSVAEERSEITPVTAIKVTPTSGTVAKGKTTTLTVSFEPESA
                     TDKTFRAVSADPSTGTIAVKDMAITVTGVKAGKVSIPVISGNGQFATVAEVTVTEAGA
                     AG"
     gene            504..>915
                     /locus_tag="ACHKAY_005763"
     CDS             504..>915
                     /locus_tag="ACHKAY_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO5704170.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNALTDDDFSAG"
BASE COUNT          229 a          204 c          289 g          193 t
ORIGIN      
        1 gggagaagaa ggtcagaaag ggcttatagg ctggtttgaa agcggggatg tgcgggccta
       61 taaaatccgt ttcccgaacg gcacggtgga tgtgttccgt ggctgggtca gcagtatcgg
      121 taaggccgta acggcgaagg aagtgatcac ccgtacggtg aaagtgacca acgtgggtaa
      181 accttctgtg gcggaagaac gcagcgaaat tacgccggtc actgcgatta aggtgacgcc
      241 gacatctggt acggtggcaa aagggaaaac aacaaccctg acggtttctt ttgagccgga
      301 aagtgcaacc gacaaaacgt tcagagcggt ttccgccgat ccgtcaacgg gaaccattgc
      361 tgtgaaagat atggcgatca ctgtgacggg ggttaaggct ggaaaagtga gtatccccgt
      421 gatttccggt aatggtcagt ttgccacggt agctgaagtc accgttactg aagcgggcgc
      481 tgcagggtaa acggaggtaa tacatgtttc tgaaaaccga acaatttgaa tataacggtg
      541 tgtccgtcac gctttccgag ctgtctgcgc tgcagcgtat cgagcatctt gccctgctga
      601 aacgacgggc agaacaggct gaagccagcg gtaacctgca ggtgagcgtg gaagaccttg
      661 tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca taaccatcca cagaaaacgg
      721 agtcaccatc aatgaatgag gctgtgatgc agatcgaaca ggaggtgctc accacctggc
      781 ctgctgatgc cattgcccgg gcggaagacg tggtgttgcg tctgtccggg atgagcgggg
      841 ctgttcatgt ggatacggat atcaccgaag tggcgaaaaa taacgcgctt actgatgatg
      901 atttttctgc gggaa
//
LOCUS       DAZFKM010000470          907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFKM010000470 DAZFKM010000000
VERSION     DAZFKM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>907)
                     /locus_tag="ACHKAY_005764"
     CDS             complement(<1..>907)
                     /locus_tag="ACHKAY_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5704171.1"
                     /translation="KPGHDYYFYICSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIG
                     KTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQV
                     DTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTSDGMQSQVLLAADRIAMVNPANGN
                     TKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNAN
                     AGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDD
                     QPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          196 a          260 c          212 g          239 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggctt
//
LOCUS       DAZFKM010000471          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFKM010000471 DAZFKM010000000
VERSION     DAZFKM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHKAY_005765"
     CDS             complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHKAY_005765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO5704172.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            complement(158..322)
                     /locus_tag="ACHKAY_005766"
     CDS             complement(158..322)
                     /locus_tag="ACHKAY_005766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO5704173.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            complement(395..763)
                     /locus_tag="ACHKAY_005767"
     CDS             complement(395..763)
                     /locus_tag="ACHKAY_005767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO5704174.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
BASE COUNT          248 a          190 c          187 g          281 t
ORIGIN      
        1 atcgcccgcg aaatgccagg ctgattagga aaacaggaaa ggggggtagt gaatgctttt
       61 gcttgatctc agtttctgta ttaatatcca ttttttataa gcgtcgacgg cctcacgaaa
      121 catcttttca tcgccaataa aagtggcgat agtgaattta gtctggatag ccataattgt
      181 ttgatccatt tttcgggact cctggctgat taagtatgtc gataaggcgt ttccatccgt
      241 cacgtaattt acgggtaatt cgttcaagta aagattcgga agggcagcca gcaacaggcc
      301 accctgcaat ggcatattgc atggtgtgct ccttatttat acataacgaa aaacgcctcg
      361 agtgaagcgt aattggtatg cggtaacgcc gcgctcaggc ggctttgata gtcatatcat
      421 ctggatcaaa tattcctgat gtatcgatat cggtaattct tattccttcg ctaccatcca
      481 ttggaggcca tccttcctga ccatttccat cattccagtc gaactcacac acaacaccat
      541 atgcatttaa gtcgcttgaa attgctataa gcagagcatg ttgcgccagc atgattaata
      601 cagcatttaa tacagagccg tgtttattga gtcggtattc agagtctgac cagaaattat
      661 taatctggtg aagtttttcc tctgtcatta agtcatggtc gatttcaatt tctattgatg
      721 ttttccagtc gtaatcaatg atgtattttt tgatgtttga catctgttca tatcctcaca
      781 gaaaaaaatc gccctcacat tagagggcaa agaagatttc caataatcag aacaagtcgg
      841 ctcctgttta gttacgagcg acattgctcc gtgtattcac tcgttggaat gaatacacag
      901 tgctta
//
LOCUS       DAZFKM010000472          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFKM010000472 DAZFKM010000000
VERSION     DAZFKM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..612
                     /locus_tag="ACHKAY_005768"
     CDS             <1..612
                     /locus_tag="ACHKAY_005768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO5704175.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            633..>905
                     /locus_tag="ACHKAY_005769"
     CDS             633..>905
                     /locus_tag="ACHKAY_005769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO5704176.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNV"
BASE COUNT          305 a          169 c          207 g          224 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataatcaat gtaagaaact aaatgatgat gaattatttc
      721 gcttacttga tgatcacaat tccttgaaaa ggatttcatc tgccagagta ttacagttaa
      781 gaggtgggca agacgctgtt agattggcaa ttgagttctg ctctgataaa aattatatcc
      841 gtagagatat cggagcattt atactcgggc aaataaaaat ttgcaaaaaa tgcgaagata
      901 atgtt
//
LOCUS       DAZFKM010000473          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFKM010000473 DAZFKM010000000
VERSION     DAZFKM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>899
                     /locus_tag="ACHKAY_005770"
     CDS             <1..>899
                     /locus_tag="ACHKAY_005770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704177.1"
                     /translation="TKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHV
                     PGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVS
                     VEVQSVTDGVKVKVSRVPEGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQ
                     HVPEKEAIVDNGAHFDGEQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVK
                     GVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDAAS
                     VSFRIAAPAAPSRIELTPGYFQIT"
BASE COUNT          188 a          242 c          313 g          156 t
ORIGIN      
        1 acgaagatgg atgcctttgg ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg
       61 ctgattaaaa cggagctgct ggagacgcag accgtggatt tcagcgtcgg cgcagaaggg
      121 cttcgccatg taccgggcga tgttattgaa atctgcgatg atgactatgc cggtatcagc
      181 accggtggtc gtgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa
      241 atcacgctgc catcctccgg taccacgctg ataagcctgg ttgacggaag tggcaatccg
      301 gtcagcgtgg aggttcagtc cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct
      361 gagggtgttg ctgaatacag cgtatggggg ctgaagctgc cgacgctgcg ccagcgcctg
      421 ttccgctgcg tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag
      481 catgtgccgg aaaaagaggc catcgtggat aacggggcgc actttgacgg cgaacagagt
      541 ggcacggtga atggtgtcac gccgccagcg gtgcagcacc tgaccgcaga agtcactgca
      601 gacagcgggg aatatcaggt gctggcgcga tgggacacac cgaaggtggt gaagggcgtg
      661 agcttcctgc tccgtctgac cgtaacagcg gacgacggca gtgagcggct ggtcagcacg
      721 gcccggacga cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg
      781 acagtccggg cggtaaatgc gtgggggcag cagggcgatg cggcgtcggt atcgttccgg
      841 attgccgcac cggcagcacc gtcgaggatt gagctgacgc cgggctattt tcagataac
//
LOCUS       DAZFKM010000474          897 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFKM010000474 DAZFKM010000000
VERSION     DAZFKM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..629)
                     /gene="fimE"
                     /locus_tag="ACHKAY_005771"
     CDS             complement(33..629)
                     /gene="fimE"
                     /locus_tag="ACHKAY_005771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO5704178.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          286 a          199 c          166 g          246 t
ORIGIN      
        1 ttgtttttta actttattat caatgagtta aatcaaacct cttctctttt taatttttcg
       61 tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact ggcggtataa
      121 cgtacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg agtatctgca
      181 ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg agtctgcgtt
      241 acggttccag cttcaatacc ggcatcgcga ataatgcgat aggcctgctg gcgagaaagc
      301 cgactcccac ggcgagaaat aaatatagcg tcggtccggt cagcgccttt ccagttagca
      361 cgttcctggg tccagcgttc cacggcttca cgctcatcaa aacgtaacgg gtgaacggta
      421 gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc aagatcctga
      481 taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa cagaataaga
      541 caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc ctgaacttct
      601 ttaccggtaa gataacgacg tttactcaca atagtagtac tcctgactga gatatatttg
      661 aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata aaaagataac
      721 cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa aaataaaata
      781 tcactgatgt gataacatct attttcattg atttgattca atataacaat agattgcagt
      841 ggattaactt ccagttgctt aaaaaataaa gaaaaaagtg cggaaacagg agaattt
//
LOCUS       DAZFKM010000475          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFKM010000475 DAZFKM010000000
VERSION     DAZFKM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..643)
                     /locus_tag="ACHKAY_005772"
     CDS             complement(<1..643)
                     /locus_tag="ACHKAY_005772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704179.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKPPKLGMYEEKYRDYRDKFNEFSAYILQMKLA"
     gene            complement(795..>890)
                     /locus_tag="ACHKAY_005773"
     CDS             complement(795..>890)
                     /locus_tag="ACHKAY_005773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO5704180.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          251 a          192 c          142 g          305 t
ORIGIN      
        1 ttgctaactt catttgtaga atatacgcag agaactcatt aaatttatct ctgtagtctc
       61 tatatttctc ttcatacatt ccaagttttg gaggtttgat aggcgtttta atttcactgc
      121 gcaacgcatc taagtttgta agatcactgt ctataatttt aagatagttt aaatctaacg
      181 aaccatcagt tttatcaagc ttagttttca gccttttaac agcctccaag gaagcatcaa
      241 gaactggtgt ctgattcttc aaagcttctg aaaaactttt ttgtgttgat tttcgagctt
      301 gttgttgagc agattttctt aactcttttt cacgcttaac ttgttctaaa agctctttac
      361 ggtcatcaga acgtgaacca aaaaatctat cagcaagttc agttaacaaa ttagatataa
      421 tagttttcag ttctcttgca gcctggtttc ttatgaatcc ctctctcccc gacttatctt
      481 tcagctcttt attactggat tgagtaattc caatataacc aaaaatcctt ctattagacc
      541 aataatatcg ccctgcattc catgaacgtc tttcttctat ctggaagaaa tcattatcta
      601 ctcgaccata aggtaatact ctcaagctat ccctaaaaat cattagtcct gcatactttt
      661 tggccttaag atcaaagtgg gaatgttcac gttcagtatg tgatgtattt tgtgaaagga
      721 attcaaacgt tcctatctga agctcaaatg ggccgacccc tgcgtgatcc tacccacgta
      781 atatggacac aggcctaagc gaggttcttg ttttcaaatt gttccggact gaggccgcca
      841 caccaactgt gccgccgcca ccgattgtaa tcacattcga tataattaaa
//
LOCUS       DAZFKM010000476          886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFKM010000476 DAZFKM010000000
VERSION     DAZFKM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>886
                     /locus_tag="ACHKAY_005774"
     rRNA            <1..>886
                     /locus_tag="ACHKAY_005774"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          209 a          202 c          279 g          196 t
ORIGIN      
        1 cgaagccccg gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt
       61 cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc aggctgtctc cacccgagac
      121 tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg cggcaagacg gaaagacccc
      181 gtgaaccttt actatagctt gacactgaac attgagcctt gatgtgtagg ataggtggga
      241 ggctttgaag tgtggacgcc agtctgcatg gagccgacct tgaaatacca ccctttaatg
      301 tttgatgttc taacgttgac ccgtaatccg ggttgcggac agtgtctggt gggtagtttg
      361 actggggcgg tctcctccta aagagtaacg gaggagcacg aaggttggct aatcctggtc
      421 ggacatcagg aggttagtgc aatggcataa gccagcttga ctgcgagcgt gacggcgcga
      481 gcaggtgcga aagcaggtca tagtgatccg gtggttctga atggaagggc catcgctcaa
      541 cggataaaag gtactccggg gataacaggc tgataccgcc caagagttca tatcgacggc
      601 ggtgtttggc acctcgatgt cggctcatca catcctgggg ctgaagtagg tcccaagggt
      661 atggctgttc gccatttaaa gtggtacgcg agctgggttt agaacgtcgt gagacagttc
      721 ggtccctatc tgccgtgggc gctggagaac tgaggggggc tgctcctagt acgagaggac
      781 cggagtggac gcatcactgg tgttcgggtt gtcatgccaa tggcactgcc cggtagctaa
      841 atgcggaaga gataagtgct gaaagcatct aagcacgaaa cttgcc
//
LOCUS       DAZFKM010000477          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFKM010000477 DAZFKM010000000
VERSION     DAZFKM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..184)
                     /locus_tag="ACHKAY_005775"
     CDS             complement(<1..184)
                     /locus_tag="ACHKAY_005775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5704181.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHKAY_005776"
     CDS             complement(184..399)
                     /locus_tag="ACHKAY_005776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO5704182.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..>869
                     /locus_tag="ACHKAY_005777"
     CDS             542..>869
                     /locus_tag="ACHKAY_005777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704183.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNN"
BASE COUNT          244 a          232 c          170 g          223 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataaca
//
LOCUS       DAZFKM010000478          868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFKM010000478 DAZFKM010000000
VERSION     DAZFKM010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..587)
                     /locus_tag="ACHKAY_005778"
                     /pseudo
     CDS             complement(67..587)
                     /locus_tag="ACHKAY_005778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            631..711
                     /locus_tag="ACHKAY_005779"
                     /pseudo
     CDS             631..711
                     /locus_tag="ACHKAY_005779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          244 a          168 c          192 g          264 t
ORIGIN      
        1 cgttaaaatt taaccatacg gtaaaatgat tcaggaaaac cggttcagca tagcctaggc
       61 tgaacttttg aggatatcaa cgaacggaat ttttgaacat ttatgagtag atcgttgttg
      121 aagggcttca atgagcatgt caagttcatc aactgctggt ctacgattac gacggtttga
      181 tttaccaatc aaagcaagtt taagtagata tggacgagca cttttcgccg ggtttgatgt
      241 gtaattaatt ccgtatatag gtttggctgc atccagaaca ctgccaagat aactaacatc
      301 gtgactgact gttgctggac ctgcaccagc gttgtttctc agcctgcaat gttcaattac
      361 gtcattttct gtcagttcag atagtttgat cgcggagatg tcactatcca taagcagttc
      421 tagcacatat cttttagtac ggtctgcttt acctccggca tttggtcatt taaatatttg
      481 tgtagtaagt cacggactgt aagtccgtca acagcatttg atgatgaaat gccatataga
      541 tctaatacca tcactttctg tgtgccccat gttttagcat gagcatgaac gcggcggcta
      601 tctcacgggt gatcatccct ttggcatact gaaaagtatc gggtgttact gaaccgtgtt
      661 gatacatcaa ctgcactgga tattgagtgg ccgataatac cgacgttata acctataaat
      721 gccaatcagg ctgaatgttg aaaggataga atgaacagcc cgacacacaa aaaacggagt
      781 ccggctccgg tttttgtgtt gccatataag gcagatgttt gttacagcta tttaagtctg
      841 gagttcaaat taaaataggg agttttgt
//
LOCUS       DAZFKM010000479          862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFKM010000479 DAZFKM010000000
VERSION     DAZFKM010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            253..837
                     /locus_tag="ACHKAY_005780"
     CDS             253..837
                     /locus_tag="ACHKAY_005780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5704184.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          257 a          174 c          167 g          264 t
ORIGIN      
        1 atcaatccca tcgccatacc ctaaccaatt gcacttgtga atttattttt gcttaatttt
       61 gaattaagaa aatttaattt gttcaaaagc ccttaaagtg cacaataggc gtcctaatga
      121 acaatggttg taaattttgg taacaaatat cattgctcat taatatctag catctgctta
      181 taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat ggaattttat
      241 tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat gctggcaatg
      301 gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag tggtctggtt
      361 gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct gatcctgctg
      421 caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atgacaccgt tggcgctaaa
      481 gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc aggtagtaca
      541 gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct gaacaacgac
      601 atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat tgatggttcc
      661 actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc cctggatgca
      721 acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt cgcagaccaa
      781 acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta tcagtaagta
      841 ttcaccaatt aaattgattg aa
//
LOCUS       DAZFKM010000480          861 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFKM010000480 DAZFKM010000000
VERSION     DAZFKM010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..251
                     /gene="etgA"
                     /locus_tag="ACHKAY_005781"
     CDS             <1..251
                     /gene="etgA"
                     /locus_tag="ACHKAY_005781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO5704185.1"
                     /translation="LDLLSKFNISEDDLLNDACINISVAGYILASNIKSRGNTWDAVG
                     AYNAGYFNTPNAVELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(248..>861)
                     /gene="escU"
                     /locus_tag="ACHKAY_005782"
     CDS             complement(248..>861)
                     /gene="escU"
                     /locus_tag="ACHKAY_005782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO5704186.1"
                     /translation="VFKLVIITLIFYFMGHSYANEFANFTRLNAYQTLVVVAFFVFLL
                     WKGVLFGYLLFSVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEI
                     QSGSLANNIKKSTVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYD
                     IPVIEDITLARSLYKNIHKGQYITEDFFEPVAQLIRIAIDLDY"
BASE COUNT          289 a          154 c          143 g          275 t
ORIGIN      
        1 atttagattt attaagcaaa tttaatatat ctgaagatga tttattgaat gatgcctgta
       61 taaacatatc tgttgctggt tatatcttag cctctaacat aaaatccagg gggaatacat
      121 gggatgcagt tggtgcgtat aatgcaggct actttaacac accaaatgca gtagaactca
      181 gaagacaata tgcaatgaaa atatataaaa catacactaa attaaaaaac aatgaacaaa
      241 ttatcgatta ataatcaaga tctattgcta tacgaatcaa ttgtgctaca ggttcaaaaa
      301 agtcttctgt tatgtattgt cctttatgta tgttcttata aagacttctt gccaaagtaa
      361 tatcttcaat cactggaata tcatagagtt cagccagttt aatgatctgt agagctttgg
      421 catcttttcc tgtttcaata actaaaggca atggagtctc cccgagttta tagtataggc
      481 aaatcgcaat gtgagtcggg tttttgacga tcacggttga ttttttgatg ttattagcca
      541 gacttccact ttgtatctca gaatgaaggc gacggcgctc ccctttaatt tcagggttac
      601 catcagtatc cttggcttct cgtttcacct catctttact cattttcatt ttcttcagtc
      661 cctcatgctt ctggaaccag aaatcaaata ctgaaaagag tagatatccg aataacacgc
      721 ctttccataa aagaaaaaca aaaaaggcaa caacgacaag agtttgatat gcgttcagtc
      781 ttgtgaaatt tgcaaactca tttgcatatg aatgccccat aaagtaaaaa atgagagtaa
      841 ttatcactaa cttaaatact g
//
LOCUS       DAZFKM010000481          857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFKM010000481 DAZFKM010000000
VERSION     DAZFKM010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..405
                     /locus_tag="ACHKAY_005783"
     CDS             <1..405
                     /locus_tag="ACHKAY_005783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5704187.1"
                     /translation="KARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLML
                     LVNRDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDT
                     GFQQVLERADYLAKEHTKVHCTGERNFGCSRG"
     gene            571..>857
                     /locus_tag="ACHKAY_005784"
     CDS             571..>857
                     /locus_tag="ACHKAY_005784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO5704188.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKA"
BASE COUNT          197 a          237 c          242 g          181 t
ORIGIN      
        1 aaagcccgaa tcgacgctta ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc
       61 acaaccctgc attacgtctg ggcccgggag tttggggagt gcaaaggtaa aaaacactat
      121 cacctgatgc tgctggtcaa ccgggatacc tggtgtcgtg ccggtgatta ccgcgctccg
      181 ggatcactgg ccgggatgat taaacaggcg tggtgcagtg ccctgggagt ggatgtcggg
      241 tgccatgcca cgctggtgca ttttccggcc tggccggcgg tgtggctggc gcgtaatgat
      301 gacaccggct ttcagcaagt gctggaacgt gctgactatc tggcgaagga gcataccaaa
      361 gttcactgca ccggtgagcg caactttggc tgcagtcgtg gctgagccag acagacgagt
      421 tatctgactt cagtgtgatg acgtgttcac ggccaccaca ctgattttcc cttatgagac
      481 tgaactgaaa ccgccattca caccggacca cgctgcccgt accgggacgg ctgttgcctg
      541 cgtgtcgtct gacggtaagg aacatatact atgtacgcaa aatcctttct cgctcttgat
      601 ggcaacggac gtctgacggg cgcccgtaca gcacaggccg caccttatgc tcactacacc
      661 tgccacttgt gtggcagtgc actcagatac catccgcaat acgacactga acttccctgg
      721 tttgaacaca ctgacgacag gctgacagag cacggtcaac agtgccctta tgtcaggccg
      781 gagcgcagag aaatacagtt gattaaacgt ctgcagcaat tcgtaccgga tgccttaccc
      841 gtggtgcgta aagccag
//
LOCUS       DAZFKM010000482          852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFKM010000482 DAZFKM010000000
VERSION     DAZFKM010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..606)
                     /locus_tag="ACHKAY_005785"
     CDS             complement(<1..606)
                     /locus_tag="ACHKAY_005785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5704189.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRI"
     gene            complement(658..>852)
                     /locus_tag="ACHKAY_005786"
     CDS             complement(658..>852)
                     /locus_tag="ACHKAY_005786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5704190.1"
                     /translation="DRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCWGEDED
                     KPEVAPFAALEDALSLAAMS"
BASE COUNT          186 a          281 c          197 g          188 t
ORIGIN      
        1 gatccggtct ccgctttcat tgaccgaatt aaattcctca ccccgtaaca caccctgtgc
       61 cagcgcctgg ctgaactggg tgatcaccga gcccgcctct gccgtactgg caccggatat
      121 tttcagccct gtcgaaatgg cctccgtcac cttcagtaca tcaccggcac tgtaaccata
      181 ttcacgcatc gaggcagccg aacgggcaaa cagggccgca ttatccgaaa atgccgtgcc
      241 tgtccgctgg ctgatatcca tcagcacttt ctgtgatgac gaaaattcat cagatgactg
      301 cgacgcctgt ttcagacggg cattcacgga actccactca tcagccagtg aaatcaggtg
      361 tccggtggca aaggcaccgg caaatgcgcc agccattccg acagcagaac cgcgaatttc
      421 cgtcaactgg ctgttcagtt ctgccagggc acgtcgctgc tcccgggctg ccgcagcggc
      481 ctgacgcccg ccattctgca gggtccggta atattcactg cccatacggg acgcccgctg
      541 gatctccgac tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt
      601 cgccattcac ctttctccgg gcaaaaaaaa cctgccacag caggttttca tcattattta
      661 tgacattgct gcaaggctca gcgcgtcttc cagcgccgca aacggagcca cctccggctt
      721 atcctcatcc tcgccccagc agagcatggc gtccttcagt gcaacattca tcccctgtgc
      781 cccgaaaacc gctttcacga tctgtgcatt acggatatcc ccgcgctcat cacccagcgg
      841 ggataccctg tc
//
LOCUS       DAZFKM010000483          850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFKM010000483 DAZFKM010000000
VERSION     DAZFKM010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..575
                     /locus_tag="ACHKAY_005787"
     CDS             42..575
                     /locus_tag="ACHKAY_005787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5704191.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT          224 a          185 c          242 g          199 t
ORIGIN      
        1 atatccaggg atttgaaatc gataaaccct gataaatatc catgaacgca aaaatcagat
       61 acggcctgtc ggctgccgtt ctggcgctga ttggtgcagg ggcgtctgcg cctgaaatcc
      121 tcgaccagtt tcttgacgaa aaagaaggta accacaccac ggcataccgt gatggtgcgg
      181 ggatctggac catctgccgc ggtgccatcc tggtggatgg taaacctgtc gtcccgggca
      241 tgaagttgtc gaaggaaaaa tgcgaccagg ttaacgccat tgaacgtgat aaggcgctgg
      301 catgggtgga gaaaaacatc agagtgccac tgaccgaacc ccagaaagcg gggattgcgt
      361 cattctgtcc gtacaacatt ggccccggta agtgtttccc gtcgacgttt tacagacgga
      421 ttaatgccgg tgaccgcagg ggagcatgcg aggcgattcg ctggtggatt aaggacggtg
      481 gcagagactg ccgtattcgc tcaaacaact gctacggtca ggtctcacgg cgtgaccagg
      541 agagcgcgct ggcgtgctgg ggaattgaca gataagcaga atattttgct gaaaaatgcg
      601 atttgctcac gcggacggat aacacgaaat cctgcgaact gacaaaaact aagtgaataa
      661 aagtaaaaac cccgtttgtt ggctgcaagc ggggttttgt gtttcctgac tccggaaaag
      721 tcaaaggaga aagtgtgttt gattttagca aactgattcg ggagattcga atgatggctg
      781 aaaaattatc cacctggaag ttcatcctta tctggctggt gtttgtgatt atggcttccg
      841 gttatttcat
//
LOCUS       DAZFKM010000484          846 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFKM010000484 DAZFKM010000000
VERSION     DAZFKM010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>846
                     /locus_tag="ACHKAY_005788"
     rRNA            <1..>846
                     /locus_tag="ACHKAY_005788"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          239 a          187 c          261 g          159 t
ORIGIN      
        1 ggaaaggcgc gcgatacagg gtgacagccc cgtacacaaa aatgcacatg ctgtgagctc
       61 gatgagtagg gcgggacacg tggtatcctg tctgaatatg gggggaccat cctccaaggc
      121 taaatactcc tgactgaccg atagtgaacc agtaccgtga gggaaaggcg aaaagaaccc
      181 cggcgagggg agtgaaaaag aacctgaaac cgtgtacgta caagcagtgg gagcacgctt
      241 aggcgtgtga ctgcgtacct tttgtataat gggtcagcga cttatattct gtagcaaggt
      301 taaccgaata ggggagccga agggaaaccg agtcttaact gggcgttaag ttgcagggta
      361 tagacccgaa acccggtgat ctagccatgg gcaggttgaa ggttgggtaa cactaactgg
      421 aggaccgaac cgactaatgt tgaaaaatta gcggatgact tgtggctggg ggtgaaaggc
      481 caatcaaacc gggagatagc tggttctccc cgaaagctat ttaggtagcg cctcgtgaat
      541 tcatctccgg gggtagagca ctgtttcggc aagggggtca tcccgactta ccaacccgat
      601 gcaaactgcg aataccggag aatgttatca cgggagacac acggcgggtg ctaacgtccg
      661 tcgtgaagag ggaaacaacc cagaccgcca gctaaggtcc caaagtcatg gttaagtggg
      721 aaacgatgtg ggaaggccca gacagccagg atgttggctt agaagcagcc atcatttaaa
      781 gaaagcgtaa tagctcactg gtcgagtcgg cctgcgcgga agatgtaacg gggctaaacc
      841 atgcac
//
LOCUS       DAZFKM010000485          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFKM010000485 DAZFKM010000000
VERSION     DAZFKM010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..746
                     /locus_tag="ACHKAY_005789"
     CDS             <1..746
                     /locus_tag="ACHKAY_005789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO5704192.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZFKM010000486          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFKM010000486 DAZFKM010000000
VERSION     DAZFKM010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>826
                     /locus_tag="ACHKAY_005790"
     CDS             <1..>826
                     /locus_tag="ACHKAY_005790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO5704193.1"
                     /translation="TTNSYIKERDKLSGIIHNIENSIISGHESSDIIRSHQHEWGDLS
                     TVEQFKKFEFYVKSELSFSKSIFDDIKTKYITDPETKRNALYHQLDSDIKERIAFLDI
                     SHYAYPGSLLEKLQLSGYVFSDINIIAEYLLSSYGISGHYSHGVVYPAPSDKLFELLR
                     RHTNSNSDWIEKIIPYVYDILSGNVSSFLHPPLSEEQKKILSDIKLEISESVSEQYFM
                     KLTEQKSSVIGIKYSVDFDRYNENLFLSLPINQNLTLPFMYRYFEMLYDIHIGILEN"
BASE COUNT          305 a          134 c          124 g          263 t
ORIGIN      
        1 acaacaaatt catatattaa agagagggat aaactatcag gcattataca caacatcgaa
       61 aattctatta tatcaggtca tgaatcatct gatataatac gaagccatca acatgagtgg
      121 ggagatttat caacggtaga gcaatttaaa aaatttgaat tttatgttaa aagtgagtta
      181 tcattttcca agtcaatttt tgatgatata aaaaccaaat atataacaga tcccgaaaca
      241 aaacgaaacg cactttacca tcaattagat agtgatatca aagagcgaat agcctttctc
      301 gacatatccc actacgcata tccaggtagt ttattagaaa aattacagtt aagcggctac
      361 gttttttctg acatcaacat aattgcagaa tacttattgt ctagttacgg aatttctggg
      421 cattattctc acggcgtggt atacccagct ccatctgata aattatttga gttattaaga
      481 agacatacaa attccaattc tgattggata gaaaaaataa tcccatatgt ttatgatatc
      541 ttatcaggta atgtgtccag cttcttgcac cctcccttat cagaagaaca gaaaaaaata
      601 ttgagtgata taaaattaga aattagtgaa tctgtatcag aacagtattt catgaagttg
      661 acagaacaaa aatcatcagt tattggcatt aaatactctg ttgattttga tagatacaat
      721 gagaatttat ttttgtcttt acctataaat caaaatttaa ctctaccttt catgtatcgc
      781 tacttcgaga tgttatatga cattcacatt gggatcttag aaaaca
//
LOCUS       DAZFKM010000487          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFKM010000487 DAZFKM010000000
VERSION     DAZFKM010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..540
                     /locus_tag="ACHKAY_005791"
     CDS             <1..540
                     /locus_tag="ACHKAY_005791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704194.1"
                     /translation="EMLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGA
                     GGFIGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEA
                     IMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLID
                     QRVQAVMLSMRRDGGMLSG"
     gene            533..>819
                     /locus_tag="ACHKAY_005792"
     CDS             533..>819
                     /locus_tag="ACHKAY_005792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704195.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVT"
BASE COUNT          176 a          186 c          293 g          164 t
ORIGIN      
        1 gagatgctga acggaaacaa agtggaatgg cgcaactggg cctcatccgt actgcaggaa
       61 atctcaaaag ttcttatgaa tgccgcgatt gtcaacggaa ttaagacggc ggcaaacggt
      121 atgtccggtg cgggaggatt tattggcagc attggtgact ggctgggcgg tgcggtggcc
      181 aatgcaaaag gcggcgtgta tacctcggca aacctgagtg cgtacagcaa cagcattgtg
      241 gacacgccca cgtactttgc gtttgcaaaa ggggccgggc tgatggggga agccggacct
      301 gaagccatta tgcccctgac ccgggcggcg gatggctcgc tgggcgtacg cgcggtgggc
      361 agtatgaacg gcagtgccgg tctggtgtat tccccggtct accacatcgc cattcagaat
      421 gacggggcta acggacagat aggggcggag gcggcaggca gtcttgtgca gctgattgac
      481 cagcgggtgc aggcggtgat gctgtccatg cgacgtgacg gaggaatgct gagtggctga
      541 gataaaaacg ctgcatctgg tcccgcgtga agggatgcag gtgagtgaga aaccgtcggt
      601 ggtgagggtt cggtttggtg acggttatga acagcgccga ccgacgggac ttaatgccag
      661 actgaagacg tttcaggcgg tgttccgggt gacggatgaa ccaacccggc gctggctgga
      721 tgaattttta tcctggcatg gtggttaccg tgcctttttg tggcgaccgc cgaaacataa
      781 ccggacggtg agggtggtgt gccgggagtg gagcgtcac
//
LOCUS       DAZFKM010000488          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFKM010000488 DAZFKM010000000
VERSION     DAZFKM010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..419)
                     /locus_tag="ACHKAY_005793"
     CDS             complement(<1..419)
                     /locus_tag="ACHKAY_005793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFO5704196.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPEL"
     gene            577..>818
                     /locus_tag="ACHKAY_005794"
     CDS             577..>818
                     /locus_tag="ACHKAY_005794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO5704197.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKG"
BASE COUNT          186 a          259 c          202 g          171 t
ORIGIN      
        1 tccagctccg gcgcggccag aatacgcgga cggtatccga tgctttcatc ctgctctgca
       61 acaagcagcg catacatccc cgtatagctg ccgtcagatt cagaaccacc gataaccagt
      121 tgatcctgcg tctttccgtc ttcttctttg tgttcagcca cgcgaacgac gatcaccttt
      181 gtgctcacct ggtctgcaat ggccttaagc gcacgataaa gcgtccccgt tgtcccgcat
      241 tttcccagca cgtcattgac gcgggtcagc agtgtgggct tgttcagcgg gaacagctcc
      301 gcgtccgcat catccgccgt tgccacgata ccgataacac tggaatcaac atcattaatc
      361 gctgttacca ggtcggtact ttccgtaaca cgggcaccat gaaaacgagt ttcactcata
      421 gcttcagccc cttgtatccg ttaaatgatt cggcaacaat catcacccac cacgcgcgta
      481 atctcactcc tgcgccgttc tcccgccacg gcgacaacaa aaagcagtaa ccccctccgc
      541 acgcacatgc gaccatgccg cacagggagg gaacagatga ccgacaccac catgcaattg
      601 ctcagtcagg gcacagaccc cgtgaaaatg ccggattttg atattctcgc ggagggtaaa
      661 acgctgtcag gcgtggcaga gcgcctgatg agcctgtcac tgaccgacaa ccggggattt
      721 gaagcggacc agctcaccat cacgctggat gatgcggatg gtcagttgca gctaccgcca
      781 cggggcgcgc gcctgacggt tctcattggc tggaaagg
//
LOCUS       DAZFKM010000489          818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFKM010000489 DAZFKM010000000
VERSION     DAZFKM010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..419)
                     /locus_tag="ACHKAY_005795"
     CDS             complement(<1..419)
                     /locus_tag="ACHKAY_005795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704198.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPEL"
     gene            577..>818
                     /locus_tag="ACHKAY_005796"
     CDS             577..>818
                     /locus_tag="ACHKAY_005796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO5704199.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKG"
BASE COUNT          182 a          261 c          203 g          172 t
ORIGIN      
        1 tccagctccg gcgcggccag aatacgcgga cggtatccga tgctttcatc ctgctctgca
       61 acaagcagcg catacatccc cgtatagctg ccgtcagatt cagaaccacc gataaccagt
      121 tgatcctgcg tctttccgtc ttcttctttg tgttcagcca cgcgaacgac gatcaccttt
      181 gtgctcacct ggtctgcgat ggccttaagc gcacgataaa gcgtccccgt tgtcccgcat
      241 tttcccagca cgtcattgac gcgggtcagc agtgtgggct tgttcagcgg gaacagcttc
      301 gcgtccgcat catccgccgt tgccacgata ccgataacgc tggaatcaac atcgttaatc
      361 gctgttacca ggtcggtatt ttccgtaaca cgggcaccat gaaaacgagt ttcactcata
      421 gcttcagccc cttgtatccg ttaaatgatt cggcaacaat catcacccac cacgcgcgta
      481 atctcacccc tgcgccgttc tcccgccacg gcgacaacaa aaagcagtaa ccccctccgc
      541 acgcacatgc gaccatgcca cacagggagg gagcagatga ccgacaccac catgcaattg
      601 ctcagtcagg gcacagaccc cgtgaaaatg ccggattttg atattctcgc cgagggtaaa
      661 acgctgtccg gcgtggcaga acgcctgatg agcctgtcac tgaccgacaa ccggggattt
      721 gaggcggacc agctcaccat cacgctggat gatgccgatg gccagttgca gctaccgcca
      781 cggggcgcgc gtctgacggt tctcattggc tggaaagg
//
LOCUS       DAZFKM010000490          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFKM010000490 DAZFKM010000000
VERSION     DAZFKM010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..224
                     /locus_tag="ACHKAY_005797"
     CDS             <1..224
                     /locus_tag="ACHKAY_005797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704200.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHKAY_005798"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHKAY_005798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            673..>813
                     /locus_tag="ACHKAY_005799"
     CDS             673..>813
                     /locus_tag="ACHKAY_005799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO5704201.1"
                     /translation="MAIPYEPYGDITMTYKYNPFWQQRIRETVRHALNVHPRLTALRV
                     DLR"
BASE COUNT          214 a          211 c          204 g          184 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgt
//
LOCUS       DAZFKM010000491          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFKM010000491 DAZFKM010000000
VERSION     DAZFKM010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>813
                     /gene="espX1"
                     /locus_tag="ACHKAY_005800"
     CDS             <1..>813
                     /gene="espX1"
                     /locus_tag="ACHKAY_005800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO5704202.1"
                     /translation="ELVKVFCEYKTEFLILFDDFFRSHNLPKPSPVLHHFFQYTHLRD
                     AYFYRCKLIEHTVQFYFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVNFLK
                     ANFNQEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIK
                     KNIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYSQLCKKLSLFKSIPSRLIKK
                     SAYCSTENTITNKFDVVFSYCGNNVKEFILLLPYNKSLEMHELNEQNIQYLTA"
BASE COUNT          310 a          140 c          111 g          252 t
ORIGIN      
        1 tgagttagta aaagtatttt gcgagtataa aacagagttc ttaattttgt ttgatgactt
       61 ttttagaagc cataacttgc ctaaaccatc tccagtcctt catcattttt ttcaatatac
      121 tcatctgaga gatgcatatt tttatagatg taaactcata gaacatacag ttcaatttta
      181 tttttttaaa cacaaaggta tcacattact tagattggat gtttttgatg acagaacaag
      241 cgaatgtctt tctgaagaga ttaagatata ccaagaatgc catgaaaaat tcgttaattt
      301 tttaaaggca aacttcaacc aagaaatata cccacagcta tatactcctg aaatatttta
      361 tgaggcctgc agaaatctcc agtcatttta tgatcatcag gaaacgtcac aaaacgcaaa
      421 atacagtgca atagacaaga aaaaaagtca tcttaaccag gaaatcaaaa agcttattaa
      481 aaaaaatatt ttccctgagc tatacaatga gcaatgcaat aaaatcccct cctcatcgat
      541 agatgataac cagggaataa tgtggcagaa ctttaaaact agcaatattg catattcaca
      601 actatgtaaa aaattatctt tatttaaaag catacccagt cgattaataa aaaaatcagc
      661 ttactgctca actgaaaaca caatcaccaa taaatttgat gttgtctttt cttactgcgg
      721 aaataatgta aaggaattta tacttttatt accctataat aaatctctgg agatgcatga
      781 acttaacgag caaaacatcc agtatttgac tgc
//
LOCUS       DAZFKM010000492          811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFKM010000492 DAZFKM010000000
VERSION     DAZFKM010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..642
                     /locus_tag="ACHKAY_005801"
     CDS             <1..642
                     /locus_tag="ACHKAY_005801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704203.1"
                     /translation="RLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLR
                     GRISGTQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASA
                     VSGKIAGALVEEDRVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEII
                     FAAGIQQRAATNMLIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            718..>811
                     /locus_tag="ACHKAY_005802"
     CDS             718..>811
                     /locus_tag="ACHKAY_005802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704204.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYI"
BASE COUNT          190 a          197 c          227 g          197 t
ORIGIN      
        1 cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa caaagtgatg
       61 aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc tgacatgatc
      121 aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc cgtggcatct
      181 tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt cagtccactg
      241 ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg aatgattgcc
      301 tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt caaaattctg
      361 gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac cggacatgag
      421 attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct ggagatcatt
      481 ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc actggtggtc
      541 agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg caatatggtt
      601 aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt ctgcgccttt
      661 tacgctttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt aaataccatg
      721 attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac gtggtctgcc
      781 ccactccggg cacaggatca acgttacatc a
//
LOCUS       DAZFKM010000493          810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFKM010000493 DAZFKM010000000
VERSION     DAZFKM010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..382)
                     /locus_tag="ACHKAY_005803"
     CDS             complement(68..382)
                     /locus_tag="ACHKAY_005803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5704205.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          237 a          207 c          116 g          250 t
ORIGIN      
        1 acacaaaaag cgaccactaa agtcgctttt tcttatggta acaggcaata acgctctcag
       61 atatttttta gcattttttt gaccgcgcgt ttccggacgt attctgttct cctgtccctt
      121 tatatcgtcg gaatacccgc cgctcttcaa atcccattcc caactcagaa tgtagtctgt
      181 tgaccgcttg ttttatttcg gtcaggttca ccggtgaaac cggagtccgg cgcgccttac
      241 gcaaacactc tgctcgtttc tgtgccgcca cttttctttt ctggtcatca cttagctgta
      301 ccatcacttt tgcccatcgt tcagctgctc tccggtacag tccttttttc tccagacatt
      361 ctgccacgtg atcatgtagc ataagtgacc tccgattatc tacagactgc catcctgaat
      421 ttaccttccc ttaatgaaat aacaataaaa aacaaaccac gcaaaaacaa taaaacaaca
      481 cacaaaaaaa actaaataat aaacaaaaat aatcacctta ttttattatt ttttgagggg
      541 gcaattactg aacaaaaaac gctgactata tactcaaaac caaacaacta ttctgccaat
      601 caggtatcat ggcaacacac ggaattaccg tgtttttgcc ttctctgccc atacaatacg
      661 ggcatatact tcattctcta ttgtaatatt tctatccatg tgccccactc catttacctg
      721 taaataatat tcaaaatatt tatcacagaa atcgtttttg gccatgaact gagcacacta
      781 taaagtccgg aactgactct ttgttaaatt
//
LOCUS       DAZFKM010000494          807 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFKM010000494 DAZFKM010000000
VERSION     DAZFKM010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..785
                     /locus_tag="ACHKAY_005804"
     CDS             69..785
                     /locus_tag="ACHKAY_005804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO5704206.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          272 a          137 c          172 g          226 t
ORIGIN      
        1 atatgccgaa ttaacacatg ttaacgattt accagtaatg taaataaatt ttcgaggaga
       61 tcattccagt gggacgtaaa tgggccaata ttgttgctaa aaaaacggct aaagacggtg
      121 caacgtctaa aatttatgca aaattcggtg tagaaatcta tgctgctgcc aaacaaggtg
      181 aacccgatcc agaattaaac acatctttaa aattcgttat tgaacgtgca aagcaggcac
      241 aagttccaaa gcacgttatt gataaagcaa ttgataaagc caaaggcggc ggagatgaaa
      301 cgttcgtaca gggacgttat gaaggctttg gtcctaatgg ctcaatgatt attgctgaga
      361 cattgacttc aaatgttaac cgtacgattg ctaacgttcg cacaattttc aataaaaaag
      421 gcggcaatat cggagcggca ggttctgtca gctatatgtt tgacaatacg ggtgtgattg
      481 tatttaaagg aacagaccct gaccatattt ttgaaatttt gcttgaagct gaagttgatg
      541 ttcgtgatgt aactgaagaa gaaggtaata ttgttattta tactgaacct actgaccttc
      601 ataaaggaat cgcggcgcta aaagcagctg gaatcactga gttctcaaca acagaattag
      661 aaatgattgc tcaatctgaa gttgagcttt ccccagaaga tttagaaatc tttgaagggc
      721 ttgttgatgc ccttgaagat gacgacgatg tacaaaaagt ttatcataac gtcgcaaatc
      781 tctaatgata ttttaaagaa atctgtc
//
LOCUS       DAZFKM010000495          806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFKM010000495 DAZFKM010000000
VERSION     DAZFKM010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>806
                     /locus_tag="ACHKAY_005805"
     CDS             <1..>806
                     /locus_tag="ACHKAY_005805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO5704207.1"
                     /translation="AAGVTDANYYPEGMHSQKRENWREYLERERGKKPGEEKHHQRKT
                     TLPMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGG
                     RRVAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIA
                     ARQVERETGKPVRMIILDTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHH
                     SGKDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEA"
BASE COUNT          191 a          179 c          273 g          163 t
ORIGIN      
        1 gcggcgggtg taactgatgc gaattattac cccgaaggga tgcacagcca gaagcgggag
       61 aactggcgcg aatatctgga acgggagcgg gggaaaaagc caggggaaga aaaacaccac
      121 cagcgaaaaa ccacgctacc aatgagcgtg ggatctgccg gatacgacac acaacttgat
      181 tacgtggtta aggggattat tccggcggta tcgctgtgca gcatatacgg ggcgagcggt
      241 tcatataaat cgttccttgc gggttcgtgg gcgtgtcacg tttccacggg ccgccagtgg
      301 ggaggccgca gggtggcgca tggcgctgtt ctctatgtgg ttggtgaagg cggtatcggc
      361 gttccgcgtc gtgtaaaagc ctgggaggtt gtgcacggtg agcaggtgaa aaatctgtat
      421 ctggtaaacc gtcccatctt tccggctgcc ccgcttgatg ttgaggaaat ggttatcgct
      481 gcccgtcagg tggaacggga aacgggtaaa ccggttcgaa tgattattct ggatacgctg
      541 gcgcgctgct ttggcggcaa tgatgaaaat gattcccgtg atatgggggc gtttatccgt
      601 gggtgtgacg aactgaaacg gcgcacaggg gcaacggtgc tggtggttca ccattccggc
      661 aaggatgaaa caaagggggc gcgtggttcc agtgcatttc gtgcatcact ggacgccgaa
      721 taccggatac gcagggagga cgcaggcagc gaagcgctgg ttatctcatg taccaaaatg
      781 aaggacgcgg aggaactcaa agaagc
//
LOCUS       DAZFKM010000496          804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFKM010000496 DAZFKM010000000
VERSION     DAZFKM010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..594)
                     /locus_tag="ACHKAY_005806"
     CDS             complement(<1..594)
                     /locus_tag="ACHKAY_005806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO5704208.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPV"
     gene            complement(694..759)
                     /locus_tag="ACHKAY_005807"
                     /pseudo
     CDS             complement(694..759)
                     /locus_tag="ACHKAY_005807"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            730..>804
                     /locus_tag="ACHKAY_005808"
     CDS             730..>804
                     /locus_tag="ACHKAY_005808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704209.1"
                     /translation="MKHLSGIGFRIPAAPASLTFRVADN"
BASE COUNT          170 a          228 c          222 g          184 t
ORIGIN      
        1 gacgggctga tgttcgtcac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg
       61 tgccggtggc ggcagaatca gcgactgcat ggcatcacgc ttttcctcaa tccggtcaaa
      121 cacgcccacc acctcgtaag ccccttcaat gactctgtcc accacatttc cccggtgcgg
      181 aacacgcact tcccccagag actgaccaca gacgcatccg ttctggcaga cgaacctgaa
      241 gtaacccggc agcatctggt agctggaggt accgtcatga gagttgagca gaataatttc
      301 agggacatgt tctccgttta tctctccggc ccgccgcaga cgcagcatgt gttttgtgta
      361 tccccggcgg cccgggtcgc gcacacgggt ctggcaggcg aagaatggct gaaagccttc
      421 ccgctgcagg ctttccagta cggtgatggt ggggatgtac gtatagttct ggcttcggga
      481 cgtgtgttta tcttccccga aaatccccgg cacatagtgc atcagttctt cgtgtgtcag
      541 cggacggtca cggcgtatct ggttcgcata accaaaacga ctggctagtc gcataatttg
      601 ctccttatcg gtggttaaga tttactggtg taataaatga aaaagccacg tctcccggag
      661 aagacgcggc ctgatgggtg aaatgaatga cgtttattgt ctgagaagac cgtcactggc
      721 gagccgcgta ttaagcacct ttccggcatc gggttcagaa ttccggcagc gccagcgtcg
      781 ctgaccttcc gcgttgcgga taac
//
LOCUS       DAZFKM010000497          803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFKM010000497 DAZFKM010000000
VERSION     DAZFKM010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>803
                     /locus_tag="ACHKAY_005809"
     CDS             <1..>803
                     /locus_tag="ACHKAY_005809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO5704210.1"
                     /translation="AAGVQDANYYPDGMHSQKRENWREYLERERGEKQEEKGHQRKTK
                     LPMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGR
                     RVAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVDEMVIAA
                     RQVERETGKPVRMIILDTLARCFGGNDENDSRDMGTFIRGCDELKRRTGATVLVVHHS
                     GKDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEA"
BASE COUNT          184 a          181 c          270 g          168 t
ORIGIN      
        1 gcggcaggtg tacaggatgc gaattactac cccgatggga tgcacagcca gaagcgggag
       61 aactggcgcg aatatctgga acgggagcgc ggggaaaagc aggaagaaaa aggccaccag
      121 cgcaaaacaa agctaccgat gagcgttggt tctgccggat atgacacgca actggattac
      181 gtggttaagg ggattattcc ggcggtatcg ctttgcagca tatacggggc gagcggttcc
      241 tataaatcgt tccttgcggg ttcgtgggcg tgtcacgttt ccacgggccg ccagtgggga
      301 ggccgcaggg tggcgcatgg cgctgttctc tatgtggttg gtgaaggcgg tatcggcgtt
      361 ccgcgtcgtg taaaagcctg ggaggttgtg cacggtgagc aggtgaaaaa tctttacctt
      421 gtaaaccgtc ccatctttcc ggctgccccg cttgatgttg atgaaatggt tatcgctgcc
      481 cgtcaggtgg agcgggaaac gggtaaacct gttcgaatga ttattctgga tacgctggcg
      541 cgctgctttg gcggcaatga tgaaaatgac tcccgtgata tggggacgtt tatccgtggg
      601 tgtgacgaac tgaaacgacg cacaggggcc acggtgctgg tggttcacca ttccggcaag
      661 gatgagacga aaggcgcgcg cggttccagt gcatttcgtg cttcgctgga tgctgaatac
      721 cggatacgca gggaggacgc aggaagcgaa gcgctggtta tctcatgcac caaaatgaag
      781 gacgcggagg aactcaaaga agc
//
LOCUS       DAZFKM010000498          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFKM010000498 DAZFKM010000000
VERSION     DAZFKM010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..332
                     /locus_tag="ACHKAY_005810"
     CDS             <1..332
                     /locus_tag="ACHKAY_005810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO5704211.1"
                     /translation="LIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAE
                     QTHVPLITWMSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKED
                     DIISSCR"
     gene            complement(425..784)
                     /locus_tag="ACHKAY_005811"
     CDS             complement(425..784)
                     /locus_tag="ACHKAY_005811"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO5704212.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          261 a          153 c          138 g          247 t
ORIGIN      
        1 aattaattga caagcttatc gaatataaag atgagtatga tactgtttta ttatatgtct
       61 ctgaccatgg agaatctctg ggagaaaatg gattatactt acatggaaca ccatataatg
      121 ttgctccagc tgagcaaacg catgtgcctc ttattacctg gatgtctccg gggttcgttt
      181 cctctaaaaa aattgatttg aattgtttgt cagaacatgc tctaaacaga acagtaagtc
      241 atgataatat attttcatcg ttactggggt tatggaatgt aaatacttca gtttataata
      301 aagaagatga tattatatcc tcctgcagat agatactgac atttttctga ggataagagg
      361 tgaatacctc ttatccttaa gtaaatatta accaggtaac tgtgaataat ttagatgttg
      421 ggcgttatga aagtaataaa actccccata taatgatagc aactaaaatc gtaatcaaca
      481 ctgcggctga acccatatct tttgctctgc ctgataattc gtgatgttct gaaccaattc
      541 tgtcaacaac tgcttcaatt gcactgttaa ttatttcaac aataattacc atcattgccg
      601 aaccaatgag taatactcga gtaacagagt caacatccag ccaaaaggat attatcagtg
      661 ctgacagaac cagaatgcac tcctgacgaa aggctgcttc atagcgccat gccgcacata
      721 agcctttcca tgaataaccc gctgctttaa taattctggt aacaccagtg gtcgtattag
      781 ccataaatac ctcttattt
//
LOCUS       DAZFKM010000499          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFKM010000499 DAZFKM010000000
VERSION     DAZFKM010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..514
                     /locus_tag="ACHKAY_005812"
     CDS             11..514
                     /locus_tag="ACHKAY_005812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001454006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5704213.1"
                     /translation="MTGYVVRWDKLSELLWGEFYEKFQRGAFTEWLAAGNDVRGLYEH
                     DHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTSTGRDVIELVKRGDISGMSFGFRSRK
                     DVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARRSLYEQHPEKMPRADNRRW
                     WADLAGV"
     gene            516..>799
                     /locus_tag="ACHKAY_005813"
     CDS             516..>799
                     /locus_tag="ACHKAY_005813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704214.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGSGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQT"
BASE COUNT          175 a          203 c          253 g          168 t
ORIGIN      
        1 agccggtacg ctgacgggct acgttgttcg ctgggataag ctttcagaac tgctatgggg
       61 ggagttttac gaaaaattcc agcggggggc gtttactgag tggcttgcgg cgggtaatga
      121 cgttcgcggc ctgtatgagc atgaccacag catgttactg gggcgcaccc gttccggcac
      181 gctgaaactg gaagaggacg acacagggtt acgctttgaa cttaccccac cggataccag
      241 cacagggcgt gacgttatcg aactggttaa acgtggtgat atatccggca tgagctttgg
      301 ctttcgttcc cgtaaggatg tatgggatac cacaacagat ccatgcgtgc gcaccgtgct
      361 ggtggcggaa ctgtacgaaa ttaccgttac atccgtaccg gcttaccctg attccggcgt
      421 ggagctggcc cgccgttccc tgtatgagca gcaccccgaa aaaatgccgc gtgcggataa
      481 tcgccgctgg tgggcggatt tagcgggggt gtgatatgtg gcctttcaga agaaaaaaag
      541 agcagcgcag catgacgctg gatgaattta tggcgctggc cggcacatcg aacacggggt
      601 cgggtgagta cgtatcatcg gggacagcgg aatcactgcc cgccgtcatg aacgccgtca
      661 cggtcatctc tgaggcggtg gctaccatgc cgtgttacct gtacctggta cgcaatgaga
      721 aggggaagga ggcccgagag tggcttgatt ctcatccggt cgatcacatc ctcaacgagc
      781 gcccgaacgc gtggcagac
//
LOCUS       DAZFKM010000500          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFKM010000500 DAZFKM010000000
VERSION     DAZFKM010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..737
                     /locus_tag="ACHKAY_005814"
     CDS             228..737
                     /locus_tag="ACHKAY_005814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5704215.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
BASE COUNT          223 a          167 c          208 g          201 t
ORIGIN      
        1 gtgggtcctt tccggcgatc cgacaggtta cggggcggcg acctcgcggg ttttcgctat
       61 ttatgaaaat tttccgggat ccatgtccgg tttctcttca agttaactat atgaaaaata
      121 taaaaacagg tcttctgtga accggacatg aacaaaaaac agacatgtaa accggacatg
      181 accggttttg ttgtgattgt gaggtgagag tttttgcgag gtgaggagtg gctacgcaga
      241 ctgaagttgc caggcattta agtctgaccg atcgccagct tcgcagattg cagaaattac
      301 cgggtgcccc ggtctcgaat aagcgagggc aactggatct ggatgcctgg cgcgattttt
      361 acatatcgta tctgagaaga agtaaaaacg atgtgcctga tggcgatagc gaagaagact
      421 atgaagagaa attgcttatt gccagatggg aactgacagc agaacaggct gttacacagc
      481 agttaaaaaa tgaggtgtca aaaggaaaac tgattgacac cgggttctgt atttttgccc
      541 tcagtaagct ggcaatggcg ttatccagta cgcttgattc catcccttta tccatgcagc
      601 gacagtttcc tgatttaaca ccgcgccatc ttgaccatct gaaaaccctt attgctaagg
      661 gggcaaatca gtgtgcgcgg gcaggggata aattaccgga tttacttgat gaatatatca
      721 gagcaacaac tgaataatat gatgagcgct gtcacaactg cattacagcc cctgataagg
      781 gcattgccgg tgacgccag
//
LOCUS       DAZFKM010000501          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFKM010000501 DAZFKM010000000
VERSION     DAZFKM010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..517
                     /locus_tag="ACHKAY_005815"
     CDS             <1..517
                     /locus_tag="ACHKAY_005815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO5704216.1"
                     /translation="YGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGTGIWT
                     ICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTDPQKAGIASF
                     CPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQ
                     ESALACWGIDR"
     gene            595..>791
                     /locus_tag="ACHKAY_005816"
     CDS             595..>791
                     /locus_tag="ACHKAY_005816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704217.1"
                     /translation="MNKSKTPFVGRKRGFVFPDSGKVKGESVFDFSKLIREIRVMAEK
                     LSTWKFILIWLVFVIMASGYF"
BASE COUNT          195 a          175 c          235 g          186 t
ORIGIN      
        1 atacggcctg tcggctgccg ttctggcgct gattggtgca ggggcgtctg cgcctgaaat
       61 cctcgaccag tttctggatg aaaaggaagg taaccacacc acagcatacc gtgatggtac
      121 gggtatctgg accatctgcc gcggtgccat cctggtggat ggtaaacctg tcgtcccggg
      181 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgagcgtg ataaggcgct
      241 ggcatgggtg gagaaaaaca tcaaagtgcc gctgaccgac ccccagaaag cggggatcgc
      301 gtcattctgt ccgtacaaca ttggtcccgg taagtgtttc ccgtcgacgt tttacagacg
      361 aattaatgct ggtgaccgca ggggagcatg cgaggcgatt cgctggtgga ttaaggacgg
      421 tggcagagac tgccgtattc gctcaaacaa ctgctacggt caggtctcac ggcgtgacca
      481 ggagagcgcg ctggcgtgct ggggaattga cagataagca gaatattttg ctgaaaaatg
      541 cggtttgctc acacgggcgg ataacacgaa atcctgcgaa ctggcaaaaa ctaagtgaat
      601 aaaagtaaaa ccccgtttgt tggccgcaag cggggttttg tgtttcctga ctccggaaaa
      661 gtcaaaggag aaagtgtgtt tgattttagc aaactgattc gggagattcg agtgatggct
      721 gaaaaattat ccacctggaa gttcatcctt atctggctgg tgtttgtgat tatggcctcc
      781 ggttatttca t
//
LOCUS       DAZFKM010000502          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFKM010000502 DAZFKM010000000
VERSION     DAZFKM010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..374)
                     /locus_tag="ACHKAY_005817"
     CDS             complement(<1..374)
                     /locus_tag="ACHKAY_005817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5704218.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVA"
     gene            351..493
                     /locus_tag="ACHKAY_005818"
                     /pseudo
     CDS             351..493
                     /locus_tag="ACHKAY_005818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(688..>791)
                     /locus_tag="ACHKAY_005819"
     CDS             complement(688..>791)
                     /locus_tag="ACHKAY_005819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5704219.1"
                     /translation="TSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          203 a          229 c          190 g          169 t
ORIGIN      
        1 caggcgacac cgcccggtgt caccgtactg cgacgcgcca gctgcttaat gcgcgggtcc
       61 ggacggtcat atgtctccgg cagcggaagg ccttcaccat atgccatgct gtttgactgc
      121 cccgccagaa ccacaacaaa gtaatactcc gggtctctgg tggcgctgat tactgtgcct
      181 tctccatccg acggcttcac caacacaggt gtggtgacat caccttccgc cgcaatggcc
      241 tgcatcagag tataaggcgt gatggccacc gggctgccaa atggctgcca cccctccttc
      301 agtttttgtg tcagcttttc cgcaaggtct gacggcgacg ccgccctgac aacatcataa
      361 tgtttaatcg acatcgaatt tctcccgtgt acaggaacag agttaaaaag ccggaaccgg
      421 aatcaaatta caggatggcc atctgccagt ggctggtcgt aaaaaaaggc cacgccatgc
      481 gcagccggaa ataaagggat aacgatgata gtttgagaaa aacagaaaca acacttttgt
      541 ggcaaagcat ggtgccgggt gcctcccggt gaattcagta tcagcacctg aatccgcgat
      601 tatcccatat acctggttgc tgatcgcccc tccgcacagg gggattcacc atgcagaagt
      661 gtttttaata aacagcaaac aaaaaaatca agcattatgc aggctgtttc tttttatcac
      721 cggccacagc aataccacaa tgccgcagac cagcacccca tccgccagca ccgacatgat
      781 tctgctggtg a
//
LOCUS       DAZFKM010000503          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFKM010000503 DAZFKM010000000
VERSION     DAZFKM010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..275
                     /locus_tag="ACHKAY_005820"
     CDS             6..275
                     /locus_tag="ACHKAY_005820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5704220.1"
                     /translation="MNILKKIMQRLCGCGKHDDREDGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            388..778
                     /locus_tag="ACHKAY_005821"
                     /pseudo
     CDS             388..778
                     /locus_tag="ACHKAY_005821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          221 a          138 c          213 g          206 t
ORIGIN      
        1 atgacatgaa catattaaaa aaaattatgc agcgtctgtg cggttgcgga aagcatgatg
       61 accgtgaaga cggggagtta cttacagcac agctgcgact gggaccggca gacattctgg
      121 agtcagatga gaatggcatt atcccggagc aggacagggt aatcacgcag gtggtgatac
      181 tggatgcgga taaaaagcag atacagtgtg tggtaagacc gctgcaaatc ctgcgtgctg
      241 acgggacgtg ggaaaatatt ggcgggatga agtaacccga cagcttcaca aaaccggagt
      301 ccggctccgg tttttgttgt catgtaaggc agatgtttgt taaagctatt taagtctgga
      361 gtttaaatta aaatagggag ttttataatg ccgttaaatt cggagattag atcaagctca
      421 tgtttaatgg attgaatgtc cttcgagctc aagtagcatc tagcggtcga ggggagttta
      481 cattaggtaa tgagactgtc agcattgtat ttaatgaaac cgatgggcgt tttctatcca
      541 gcggcagtag tgggggattg cttactgagt tattccttta tgggtttaat aacggccctg
      601 aagctcttcg cgataggatg ctcagtatgc tttcggactc aggtgaagca caatcgcaag
      661 agagtattca ggacaaaata tctcaatgta agtttcctgt tagttcagga aatttccagt
      721 gcccgccaga gtctattcag tgtccaatta cactagagag acccgaagaa ggagtgtt
//
LOCUS       DAZFKM010000504          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFKM010000504 DAZFKM010000000
VERSION     DAZFKM010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..480)
                     /locus_tag="ACHKAY_005822"
     CDS             complement(<1..480)
                     /locus_tag="ACHKAY_005822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5704221.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     TKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     "
BASE COUNT          180 a          185 c          193 g          208 t
ORIGIN      
        1 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
       61 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc ggcgcatggt
      121 ggattcatcc gccagcattc gcatgatttc ggtgttcagc tcggggataa cggtataacc
      181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca cctgtcagga tgtagttgcg
      241 tagttctgcg gggtcagtgg tctgactgct tttacctggc ttgctgcgct cttcgtctgc
      301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagtgc gtaattcgtc
      361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttccgcgt tctccagcat
      421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctcggcat
      481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaaaactc aaccgttttt
      541 cataaggaaa tcagcaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgaga
      601 acattaacag gcggaaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
      661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
      721 ataatttaca aaaaatgaca aaaagccgga tttctccggc tgtatg
//
LOCUS       DAZFKM010000505          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFKM010000505 DAZFKM010000000
VERSION     DAZFKM010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..480)
                     /locus_tag="ACHKAY_005823"
     CDS             complement(<1..480)
                     /locus_tag="ACHKAY_005823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5704222.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     "
BASE COUNT          178 a          186 c          193 g          209 t
ORIGIN      
        1 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
       61 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc ggcgcatggt
      121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
      181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca cctgtcagaa tgtagttgcg
      241 tagttctgcg gggtcagtgg tctgactgct tttacctggc ttgctgcgct cttcgtctgc
      301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
      361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttccgcgt tctccagcat
      421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctcggcat
      481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
      541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
      601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga atactcatga
      661 ttcagttcgg gtaaaatatg ccgatcctgt cagtgaacaa cgtggaacaa ccacgaacaa
      721 ataatttaca aaaaatgaca aaaagccgga ttgctccggc tgtatg
//
LOCUS       DAZFKM010000506          763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFKM010000506 DAZFKM010000000
VERSION     DAZFKM010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..730
                     /gene="slp"
                     /locus_tag="ACHKAY_005824"
     CDS             164..730
                     /gene="slp"
                     /locus_tag="ACHKAY_005824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO5704223.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          233 a          164 c          166 g          200 t
ORIGIN      
        1 gatactcatg ccacgcccca aaatcaatgg aattgaacta aacaaaaggg atataaacat
       61 cagacaggtt tacgttacta tcaggcatat cacctcagaa tcagatgaaa actataaaga
      121 aatatctatt atggctttaa tatttgttga taaggatagt aacatgaaca tgacaaaagg
      181 tgcactcatc ctcagccttt catttttgct tgccgcatgt agttcaattc cgcaaaatat
      241 caaaggcaat aaccaacctg atattcaaaa aagttttgtt gctgttcata accagccggg
      301 gttatatgtt ggtcaacaag cgcgctttgg tgggaaggtt atcaacgtta tcaatggcaa
      361 aacggatacg ttgttagaaa tcgctgtatt accgttggat agctatgcga agcctgatat
      421 tgaagccaac tatcagggcc gactgctcgc cagacaaagc ggcttccttg atccagtgaa
      481 ctatcgtaat cactttgtta ccatcctcgg taccattcag ggtgaacaac caggctttat
      541 caataaagtc ccgtataact tcctggaagt gaatatgcag ggcatccagg tgtggcattt
      601 gagagaagtg gttaatacca cctataacct gtgggattac ggctatggtg cattctggcc
      661 ggaaccgggc tggggtgcgc cttactacac caatgcggtg agtcaggtaa cacctgagct
      721 ggtcaaataa caccaccgaa agatgcagat gctttctcag cat
//
LOCUS       DAZFKM010000507          763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFKM010000507 DAZFKM010000000
VERSION     DAZFKM010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..271
                     /locus_tag="ACHKAY_005825"
     CDS             47..271
                     /locus_tag="ACHKAY_005825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704224.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
     gene            complement(640..>763)
                     /locus_tag="ACHKAY_005826"
     CDS             complement(640..>763)
                     /locus_tag="ACHKAY_005826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFO5704225.1"
                     /translation="NFSAWVIESCRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          214 a          150 c          178 g          221 t
ORIGIN      
        1 tccgcactca gtgtactaag caggcttttt attatccgga ggatgtatga agaaattaca
       61 ggtaacggta aaaccttttc agggaacaat tccgttccgt gttttgcaac atggccgtgt
      121 tctgcttgaa gaggtgttca gaggtaaatg cactgaatgt tattcacgaa catatgaagt
      181 gaatgccacg catgaagaat tcaccgttga gtgtgtgatg aatactgata aatgccgaat
      241 ggtatccgct gaattacagc cagtgtgttg agcgacctta ttatccatgc gcggtattgt
      301 cgccgtattc ctgcattaac agagaccgca gcccgacagg gagactcctc tgcgagagtg
      361 tgcggggata atcaaaaacg atacacaccg gggtttaccg cgtaaacgga gcgcggcgtt
      421 ctcccctcat ggtcgcccat ccggtgcgat ggtggaagaa actggaatct gttcaataaa
      481 aaaactgccg tgttggagtc acagcagtaa tgtactgatt gggtagaaga ttattattgt
      541 tatgctttat ttttattcta tatggctgat tatttcaatt cggaattaat acagctaatg
      601 tctgtgattt tttataaatt cagcaatata aagaaatagt tatatgaaca gccatcgcag
      661 agcatactgt gtatcattct tttttatagt caactgacgg gcatatttta tgtctgctgc
      721 cagctcccgg cggcaagatt caatgaccca cgcagaaaaa ttt
//
LOCUS       DAZFKM010000508          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFKM010000508 DAZFKM010000000
VERSION     DAZFKM010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>760
                     /gene="sepL"
                     /locus_tag="ACHKAY_005827"
     CDS             <1..>760
                     /gene="sepL"
                     /locus_tag="ACHKAY_005827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFO5704226.1"
                     /translation="ASVFNSNSLDFELESQQLTQKNSSNSYSPLLNLQNELAMITSSS
                     LSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKELSLKNSGLLEQ
                     HDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLDLLGEDDWELALLSWL
                     GVGELNQEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLKVIIRALSFD
                     LSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTD"
BASE COUNT          247 a          109 c          164 g          240 t
ORIGIN      
        1 ccgcatctgt ttttaattct aattcattag attttgaatt ggaatctcaa caattaacgc
       61 aaaaaaattc ttctaatagt tattctccat tattaaattt acaaaatgaa ttagcgatga
      121 ttactagttc atcgctttct gaaacgattg aagggctaag cctgggatat cgcaaaggca
      181 gtgcaaggaa agaagaggaa ggtacgacaa cggagaagct gcttaatgat atgcaagagc
      241 tgcttactct gaccgatagc gataaattaa aagaactatc attaaagaac agtgggctgc
      301 tggaacagca tgatcctaat ttggctatgt ttggtaatat gccaaaaggg gagattgttg
      361 cacttatatc ttctttatta caatctaagt ttgttaagat tgaattaaaa aagaaatacg
      421 ccaaattatt attagattta ttaggcgaag atgattggga gttggctctg ctttcctggt
      481 taggggtggg tgagttaaat caggaaggta tccagaagat caaaaagctt tatgaaaagg
      541 ccaaggatga ggattctgaa aagggagcct ctttacttga ctggtttatg gagattaagg
      601 atcttccaga gcgtgagaag cacttaaagg ttattattag ggcgctatcg ttcgatctgt
      661 cttatatgtc ttcttttgaa gataaggtga aaacatcttc aattattagc gatttatgcc
      721 gggtaatcat ttttttatcg cttgataact atacagatat
//
LOCUS       DAZFKM010000509          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFKM010000509 DAZFKM010000000
VERSION     DAZFKM010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..495
                     /locus_tag="ACHKAY_005828"
     CDS             61..495
                     /locus_tag="ACHKAY_005828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO5704227.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          169 a          168 c          235 g          184 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagt
//
LOCUS       DAZFKM010000510          749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFKM010000510 DAZFKM010000000
VERSION     DAZFKM010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>749
                     /locus_tag="ACHKAY_005829"
     CDS             <1..>749
                     /locus_tag="ACHKAY_005829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO5704228.1"
                     /translation="GTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGA
                     STAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDG
                     QLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTKTLS
                     GVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQF
                     RGTQIATARARAAVYRAQQAVAAARGTEMQIA"
BASE COUNT          149 a          174 c          267 g          159 t
ORIGIN      
        1 ggtaccgctt tttcggataa tgcggccctg tttgcccgtt cggctgcctc gatgcgtgaa
       61 tatggttaca gtgctgatga tgtgctgaag gtgacggagg ccatttccac ggggctgaaa
      121 atctccggtg ccagtacggc tgaggcgggt tcggtgatca cccagttcag ccaggcgctg
      181 gcacagggtg tgttgcgcgg tgaggaattt aattcggtca atgaaagtgg tgaccggatc
      241 attcgtgcac tggctgcagg catgggcgtg gcccgtaaag atctgaaggc gatggcggac
      301 gatggtcaac tgacggcgga taaagtcgtt cctgcgttaa tcagccagct gggggtattg
      361 cgtgatgaat atgccgccat gccagaaacg gtttccggta gtatcacgaa ggtggaaaac
      421 gcctttatgg cctgggtggg cggagtgaat gaggccagcg gggtgacaaa aacgctctcc
      481 ggcgtgctga acggtgttgc cggacagatt gataatgtgg caacagctgt gggtgcgctg
      541 gttgccgtcg gggttgcccg gtactttggc aatatggcct ccggagcgat gtctgccacg
      601 gcaggacttg tgacggctgc acgtaatgaa gttgcactgg cggaagcaca gttcagggga
      661 acgcagattg ccacagcgcg ggcaagggca gccgtgtacc gtgctcagca ggccgtggcg
      721 gcagcccgcg ggacggagat gcagattgc
//
LOCUS       DAZFKM010000511          744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFKM010000511 DAZFKM010000000
VERSION     DAZFKM010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..477)
                     /locus_tag="ACHKAY_005830"
     CDS             complement(<1..477)
                     /locus_tag="ACHKAY_005830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704229.1"
                     /translation="MPLYEWLINKRLRYQYLTLLAFSILALLALYLLYRNTPKVSVNF
                     FEFYHKNLRGYLFSGFISVGSFLLSLHTFVIINLRDKVFATQEYKEIYSIATGIPIDK
                     INDSVLYKPLDNLSSFINTSILCSITTAIAQFTIGLSTNLYACLFCVWLAILTVFLL"
     gene            complement(464..>744)
                     /locus_tag="ACHKAY_005831"
     CDS             complement(464..>744)
                     /locus_tag="ACHKAY_005831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704230.1"
                     /translation="SDRTKVQQLSQNLSNIYNSMSGVAKAQVIAVNHAGIEKTIDFMN
                     CPVFFETYDFDIIADKVNGLTNDNYTTNPVFDMIKEEMLNGTNKNAFI"
BASE COUNT          253 a          114 c          128 g          249 t
ORIGIN      
        1 taaaagaaat accgttagta ttgcaagcca tacgcaaaat aagcatgcat ataaattagt
       61 tgaaagtcca atagtgaatt gtgcaattgc tgttgtgata gaacataata ttgatgtatt
      121 aataaatgag gataaattgt ctaaaggttt ataaagtaca ctatcattga ttttgtcaat
      181 aggtatacca gtggcaatgc tataaatttc cttatattcc tgagttgcaa aaaccttatc
      241 gcgtaaattt attatgacaa aggtatgcag gctcaataaa aatgagccca cggaaataaa
      301 accggagaag agatagcctc gtaagttttt atgataaaac tcaaaaaagt taacacttac
      361 tttaggtgtg tttctgtaca ataggtaaag tgcaagcaat gccaggatcg agaatgcaag
      421 cagtgtaagg tactgatacc tcaatctttt atttattagc cattcatata aaggcatttt
      481 tattcgtccc gttcagcatt tcttctttta tcatatcaaa aacagggttt gttgtataat
      541 tatcgtttgt caatccattg actttatcgg caattatatc gaaatcgtat gtttcaaaaa
      601 aaacagggca gttcataaaa tcaatggttt tttctattcc tgcatggttt actgcaataa
      661 cctgtgcttt agcaactcca ctcatagaat tataaatatt tgaaagattc tgagatagtt
      721 gttgcacttt tgttctgtcg ctag
//
LOCUS       DAZFKM010000512          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFKM010000512 DAZFKM010000000
VERSION     DAZFKM010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(57..398)
                     /locus_tag="ACHKAY_005832"
     CDS             complement(57..398)
                     /locus_tag="ACHKAY_005832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO5704231.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
     gene            complement(416..>740)
                     /locus_tag="ACHKAY_005833"
     CDS             complement(416..>740)
                     /locus_tag="ACHKAY_005833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5704232.1"
                     /translation="LKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDP
                     HHLIGHGQGGMATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALA
                     IGVLA"
BASE COUNT          207 a          206 c          137 g          190 t
ORIGIN      
        1 atattaaaat tttattaaag caacagagaa aaaaaggggg gtaattcccc ctaatattac
       61 aacattgaaa gcacaccatc cagaaaacca agagctgttt gcaactcttt tcttattgtc
      121 ccatcagaac acttctgctt ctttgcaata gttcttagtg agatgccgat aacaaaatga
      181 gcaataacca actcatactc ttcgggctta tatttccgca atcgcgcaac acagctgtca
      241 atcatgattc cttcatcatc atcgcattgt tggcgtgttt tctttccatg aggtagtaaa
      301 cctttaaacc ctgcagcaac aggctgccag tccacaccac tgttatctgc tgcagcccat
      361 gcaccccagc ggtccaatac ttcatacata tcacgcatca actttctcca caaaatcagg
      421 ccagcacgcc aattgccagt gcacgatcga taaaacgaaa tatcagctcc agctgggagc
      481 catacttctc ttcaaatgcc acggtatccg catgcagctc gtcgtgatgc tttctgcaca
      541 aaggcaacac aaagaggtca tgcgcttttg tagccattcc accctgaccg tgacctatca
      601 ggtggtgggg atcatcagca ggttttccac aacatgcgca cggctgcgtc ttaacccatc
      661 gcgtgtactt ttcattaacc cagcggcgac gttttgggcg taacataaaa gactccggcg
      721 actccggatc cactttcagc
//
LOCUS       DAZFKM010000513          732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFKM010000513 DAZFKM010000000
VERSION     DAZFKM010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..732
                     /locus_tag="ACHKAY_005834"
                     /pseudo
     CDS             5..732
                     /locus_tag="ACHKAY_005834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          193 a          203 c          197 g          139 t
ORIGIN      
        1 tcacatgaca aaaacagtat caaccagtaa aaaaccccgt aaacagcatt cgcctgaatt
       61 tcgcagtgaa gccctgaagc ttgctgaacg catcggtgtt actgccgcag cccgtgaact
      121 cagcctgtat gaatcacagc tctacaactg gcgcagtaaa cagcaaaatc agcagacgtc
      181 ttctgaacgt gaactggaga tgtctaccga gattgcacgt ctcaaacgcc agctggcaga
      241 acgggatgaa gagctggcta tcctccaaaa ggccgcgaca tacttcgcga agcgcctgaa
      301 atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaagc aatgtgccgc
      361 gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac aaggataagc
      421 acgcgtcagc agttccgcca acactgcgac agcgttgtcc tcgcggcttt tacccggtca
      481 aaacagcgtt acggtgcccc acgcctgacg gatgaactgc gtgctcaggg ttaccccttt
      541 aacgtaaaaa ccgtggcggc aagcctgcgc cgtcagggac tgagggcaaa ggcctcccgg
      601 aagttcagcc cggtcagcta ccgcgcacac ggcctgcctg tgtcagaaaa tctgttggag
      661 caggattttt acgccagtgg cccgaaccag aagtgggcag gagacatcac gtacttacgt
      721 acagatgaag gc
//
LOCUS       DAZFKM010000514          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFKM010000514 DAZFKM010000000
VERSION     DAZFKM010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>731)
                     /locus_tag="ACHKAY_005835"
     CDS             complement(<1..>731)
                     /locus_tag="ACHKAY_005835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5704233.1"
                     /translation="VGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNG
                     QLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQ
                     QMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMN
                     DVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQI
                     KGKLSANQIEGDIVKTVGKAFPRDSR"
BASE COUNT          155 a          208 c          160 g          208 t
ORIGIN      
        1 cgggagtccc gcggaaaagc cttacccact gttttgacta tatcgccttc aatctggttg
       61 gctgacagtt tccccttaat ctgacagttc tcattaattg tgacattgtt gagcgtcccg
      121 gcgttcgcat tcacactgcc actgatatct gcatttttag cggtcagctt tccgtccggt
      181 gtcagggaaa atgccggagg attaccgccg ctggtaatgg tgggagccgt caggcgtttc
      241 aggaacacgt cgttcatgaa tatctgatcg ccctgaccaa caaacatcgg ctttgtgttg
      301 ccattcgcag gattaatcat cgcaatcctg tctgccgcca gcagcacctg actctgcatt
      361 cctgctggcg tattctcaat accggcaccg atacccgcaa tataaaggcg tccgtccttc
      421 atctgttgca gcttcacagc ccacatgctg ttcagattat tatttgtatc aacctgaacc
      481 ttctgtatct gctggatcgc tgcactctgg tcttccagtt tcttattgac ggtctgcgtg
      541 atttcattac tgacatccgt gatggacgtt ctgatttccg ccaggtcagg cgcaagctga
      601 ccgttatcaa tctgagtcca caactcctga gccagatggg ttttccctat ctctcctttg
      661 aaaaaatcca gatagccgga tgcatcatca ctcggctggc caacagcctc cacaaatgcc
      721 gatttgccaa c
//
LOCUS       DAZFKM010000515          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFKM010000515 DAZFKM010000000
VERSION     DAZFKM010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..>727
                     /gene="gpM"
                     /locus_tag="ACHKAY_005836"
     CDS             17..>727
                     /gene="gpM"
                     /locus_tag="ACHKAY_005836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO5704234.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSTFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASTPQ"
BASE COUNT          169 a          203 c          225 g          130 t
ORIGIN      
        1 gtaacggaga gaaaaaatga ttactcctgc acagcaacac tggcagaacg tgatggcaca
       61 gcgcgcaggc cgggcgaatg aaggcgtgga ccacgccgcg cgtaccgcgc atgaagaggt
      121 gctgtatcgt ctgcgtctgg cacaggcccg gcttaagggc gtacaggcca gaagcgcgaa
      181 agccgccatc aaaaaagagt tgttgccgga cttttccggc tggattgagg gaacgctgga
      241 ggctgacggc gggcagcagg atgaagtgat tgccacgctg atggtgtggg cgattgactg
      301 cggcgatctt ccgcttgcgc tgcgtattgg tgcgtatgtg gtccgtcaca acctcatcat
      361 gccggataac tttggccgta ctgctgccac ggtactgacc gaagaaatct gtaacccggt
      421 attgacgcag gccgggacgg atgccgacgc ggatttgtcc acctttatcg aaccactgga
      481 cacactttgg gaaattgtcg ccaaccagga tatgccggac gaagtgcgcg ccaaattatg
      541 caaggcgtgc gcctttgccc gccgtggcct gagcgatgcg gacagcatgg cctcatcact
      601 gaagctgctg cgcgaagcga tgcacctgaa cccgaacgca ggtgtgaaac gcgagattgc
      661 aaccctttcc cgcgccctga aaaaagccga ttccgcagcc gcaccagaag atgccagcac
      721 accgcag
//
LOCUS       DAZFKM010000516          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFKM010000516 DAZFKM010000000
VERSION     DAZFKM010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..>727
                     /gene="gpM"
                     /locus_tag="ACHKAY_005837"
     CDS             17..>727
                     /gene="gpM"
                     /locus_tag="ACHKAY_005837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO5704235.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASAQQ"
BASE COUNT          166 a          206 c          227 g          128 t
ORIGIN      
        1 gtaacggaga gaacaaatga ttactcctgc acagcaacac tggcagaacg tgatggcaca
       61 gcgcgcaggc cgggcgaatg aaggcgtgga ccacgccgcg cgtaccgcgc atgaagaggt
      121 gctgtatcgt ctgcgtctgg cacaggcccg gcttaagggt gtacaggcca gaagcgcgaa
      181 agccgccatc aaaaaagagt tgttgccgga tttttccggc tggattgagg gaacgctgga
      241 ggctgacggc gggcagcagg atgaagtgat tgccacgctg atggtgtggg cgattgactg
      301 tggcgatctt ccgcttgcgc tgcgtatcgg cgcgtatgtg gtccgtcaca acctcatcat
      361 gccggataac tttggccgta ctgctgccac ggtactgacc gaagaaatct gtaatccggt
      421 actgacgcag gccgggacgg atgccgacgc ggatttgtcc gcctttatcg aaccactgga
      481 caccctccgg gagattgtca ccgaccagga catgccggac gaagtgcgcg ccaaattatg
      541 caaagcgtgc gcctttgccc gccgtggcct gagtgatgcg gacagcatgg ccctgtcact
      601 gaagctgctg cgcgaagcaa tgcacctgaa cccgaacgca ggtgtgaaac gcgagattgc
      661 aaccctttcc cgcgccctga aaaaagccga ttccgcagcc gcaccagaag acgccagcgc
      721 acagcag
//
LOCUS       DAZFKM010000517          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFKM010000517 DAZFKM010000000
VERSION     DAZFKM010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>719)
                     /locus_tag="ACHKAY_005838"
     CDS             complement(<1..>719)
                     /locus_tag="ACHKAY_005838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin B"
                     /protein_id="HFO5704236.1"
                     /translation="ASSDKENKSFNWLNPVSVGVNDITPDDESSWAVRNNDINKILFE
                     KINCHVPEKLPTSLYYEIDSRVFFHGWDNKSIQYVTEINKDLIKDINLLLTSSNVDVK
                     LLIKLDRELYAISSKIENPLALRSIRTLQLQLTNYVTSNTFEPENTINFIYDFYSKKQ
                     NDLLSAIKLFSRNDVETKIIVWYNSTMEKNVFLREVISCVLWTKKVDSYIKENKKHLS
                     TEDAEALRDYAKLKIKELFSM"
BASE COUNT          226 a          113 c           97 g          283 t
ORIGIN      
        1 catcgaaaat agttctttta tttttaattt tgcataatct cttaaagctt cagcatcttc
       61 cgtagataag tgttttttgt tttcttttat ataagagtct acttttttag tccatagaac
      121 acatgatata acctctcgga ggaatacatt tttttccatt gttgaattgt accaaacaat
      181 gatttttgtc tcgacatcat tacgcgaaaa taacttaatt gcgctaagta aatcattttg
      241 ttttttacta taaaaatcat aaataaaatt aattgtattt tcaggttcaa atgtgtttga
      301 ggttacataa ttagttaatt gcagctgtaa agtccttata gagcgcaagg ctaacggatt
      361 ctctattttg ctgcttattg catataattc acgatctagc tttattaaaa gtttaacatc
      421 aacattagac gatgttaaca ataaatttat atctttaata aggtctttat taatctcagt
      481 aacatattgt atgcttttat tatcccatcc atgaaaaaaa actcgcgagt cgatttcata
      541 gtatagggag gtaggtagtt tttcgggtac atgacagttt attttttcga ataaaatttt
      601 attaatatca ttatttctaa cagcccatga gctttcatca tccggtgtaa tatcattaac
      661 accaacactc actggattca gccagttaaa acttttattt tctttatctg aacttgcat
//
LOCUS       DAZFKM010000518          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFKM010000518 DAZFKM010000000
VERSION     DAZFKM010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>715)
                     /locus_tag="ACHKAY_005839"
     CDS             complement(<1..>715)
                     /locus_tag="ACHKAY_005839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO5704237.1"
                     /translation="AYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTR
                     DRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRI
                     SGPRQTREYGYSATGRLESVRTLAPGLDIRIPYATDPAGNRLPDPELHPDSTLTAWPD
                     NRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHG
                     EPLVESRYLYDPLGRRMAKR"
BASE COUNT           89 a          234 c          232 g          160 t
ORIGIN      
        1 ccgtttcgcc attcgccgtc ccagcgggtc gtagaggtag cggctctcga ccagtggctc
       61 gccatgctgt atccgcgtgt ggaacaccag gcggtgctgg ctgtcgtagt ggtagtggtg
      121 ggtccgctcg tcgtccgtcc gtatcacacc cgtcgggatg cggtccgtct tctccgtcag
      181 tctgccgtat tcatcatagt ggtaaacata gtgcgcatcc tctgcgatgc ggttatccgg
      241 ccacgccgtg agcgtgctgt ccgggtgtag ctccgggtcc ggcaggcggt ttcccgccgg
      301 gtcggtggca tacgggatgc ggatgtccag gcccggtgcg agggtgcgca cgctctccag
      361 cctgcccgtg gcactgtagc cgtattcccg cgtctgtcgc gggccgctga tgcgcaccag
      421 gtcgccgttg tcattccacc cgtagtcacg gtcatacacc aggctgttca ggtgctggct
      481 ctgtaactgg cctgcggggg tgtatgtgct ggtcagttca tatgcggcat tactgcctgc
      541 cctgctgccg aagctgcgca ccgtctcacg gtgcagcctg tcccgcgtat actcgaccag
      601 cggcgtcccg cccagcttca tgcccgccag gtaaccgctg ccgtaggtca gccactccac
      661 cggcggcagg ctgtccggcg tgacgcggtt tgccagcccc tgttcgttgt aggcg
//
LOCUS       DAZFKM010000519          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFKM010000519 DAZFKM010000000
VERSION     DAZFKM010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>710)
                     /locus_tag="ACHKAY_005840"
     CDS             complement(<1..>710)
                     /locus_tag="ACHKAY_005840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704238.1"
                     /translation="FPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQT
                     SIEMVTLLNTLQPDGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSDN
                     TCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDIVDLSSLDLRNV
                     DLDYYDFTDKHMANTILNPFKLNSTNFTNANMFQVNFVSSTQNATISWDYLLKITPVL
                     TSISDMYSEEKIKFVESC"
BASE COUNT          228 a          110 c          124 g          248 t
ORIGIN      
        1 aaacaacttt cgacaaactt aattttttct tcagaataca tatcgctaat gcttgttaaa
       61 acaggcgtta tttttagtaa ataatcccag gagattgtgg cgttttgtgt tgaactaaca
      121 aaattaacct gaaacatgtt ggcattagta aaatttgttg agttcaattt aaaaggattt
      181 aaaatagtat tagccatatg tttatctgtg aaatcataat aatctaaatc gacattacgt
      241 aagtccagag aggatagatc aacgatatct ccagttccac aactaaagat tacgttcaac
      301 ctcctgattt cattgtaaat atatttcaat ggacaattaa cattgatact gcctgtggat
      361 attttctggt tctttaatgg ttcattaatg gtaagttcgc aggtattgtc cgatcgttga
      421 ataatttggg cgttataacc taaaaaatcc agacgaagag acgagtcatt ataaagaatt
      481 acattttgta agtacgctaa ttctttttga aaatcaccag tgataatgca ttttccatca
      541 ggttgtaacg tgttaagtaa agtgaccatt tcaatagagg tctgttttgg tttttctata
      601 cctggtgcta gtgcttcgag gttatttcct gttgggcttt caggaatgtc attcattttt
      661 cgacggacta aatcactact aatatcatta ccaattgatg aaacagggaa
//
LOCUS       DAZFKM010000520          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFKM010000520 DAZFKM010000000
VERSION     DAZFKM010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>710)
                     /locus_tag="ACHKAY_005841"
     CDS             complement(<1..>710)
                     /locus_tag="ACHKAY_005841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704239.1"
                     /translation="SELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNNVTLGG
                     VKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSA
                     RASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSR
                     AEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQK
                     AGLSRPVWIAQAYEQQIA"
BASE COUNT          165 a          217 c          168 g          160 t
ORIGIN      
        1 tccgctatct gctgctcata cgcctgcgct atccacaccg gacgtgagag tccggctttt
       61 tgtcgctcag cagattccct gacctgctgg cggaaaatgt cctgataatc ctcgcccatc
      121 agcgccagct ctttctcata cgtgctcagt ccggcctcaa tgcgcatcac tgattcctgg
      181 acttccttga gcccgtcaat ggccattctt ccggctccaa tccactcagc ccgtgaccag
      241 gctgatcgcg cctgataaaa atcaaaacgc gcccgtggcg gacgaataat cccccgaaga
      301 agtgcctctt ccagccagca ggaaaacatc tgcgtggcca gccgggacgc aataaatttt
      361 cgtcgtccca taaaatagcg ccacgactca ttggcggagg cgcgggcact tgaataactg
      421 accttcgagt aatcccggga caactgttcg taggaaacgc caagaccggc ggcgatatac
      481 cgcagcagcg cctgttcaag cgccgaaaat ccattgtctg aatcctgcgc agtctgcagt
      541 ttcagatcat caccggggaa aaggtgcgga attttgacac cgcccagcgt cacgttattc
      601 gtgtcatacc aggtggagaa cttatccaga atattaataa gcggattatc cttctgcccc
      661 tgcggcgcac cggcgatata ttcaaaggcc ttttcggtat caagttcact
//
LOCUS       DAZFKM010000521          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFKM010000521 DAZFKM010000000
VERSION     DAZFKM010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..617
                     /locus_tag="ACHKAY_005842"
     CDS             6..617
                     /locus_tag="ACHKAY_005842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO5704240.1"
                     /translation="MFSPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQ
                     SNFMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVI
                     GKETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFN
                     TQTRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          225 a          136 c          168 g          179 t
ORIGIN      
        1 attgtatgtt tagtccaatg acaatggtag gtagatcgtt agctcaggcg gctacacaaa
       61 ctcttagacc tgcggtcacc agagctgcga cacaagcggg ggtgggagcc agtggcatga
      121 agttcgtgcc catgcagtcg aactttatga ttaatcatgg caaactgact aaccagctac
      181 ttcaggccgt ggccaaacaa acccgtagcg gtgatactca gcaatggttc aaacaggagc
      241 aaattactta tatatccaga acagtaaaca gagctattga tgactattgc atgagtaata
      301 attcggtaat tggtaaggaa accaaaagcc acattttcaa ggctgttgaa agcgcgataa
      361 agcagcctct cgatatgaat tgtgcacaaa cgtcgattgg ccatttctta cagtccaaca
      421 aatactttaa taaaaaagtt gatgagcagt gtggcaaagg ggtggatcca attacacgct
      481 ttaacaccca gaccaggatg atagaacaag tctcacaaga aatctttgaa cagaaatttg
      541 gtggtttgaa agttagtgat atcaaagctc tgactcagag cacgattgca cagcatgtac
      601 aggatactcg gttgtaggaa taacctgtta tatatgcgtg ttgggcataa gttaagcttc
      661 ctggtgtata gcgtagtctt tgctgatgta agaaaaggct atatacgg
//
LOCUS       DAZFKM010000522          707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFKM010000522 DAZFKM010000000
VERSION     DAZFKM010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..424)
                     /gene="traA"
                     /locus_tag="ACHKAY_005843"
     CDS             complement(65..424)
                     /gene="traA"
                     /locus_tag="ACHKAY_005843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO5704241.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(457..684)
                     /gene="traY"
                     /locus_tag="ACHKAY_005844"
     CDS             complement(457..684)
                     /gene="traY"
                     /locus_tag="ACHKAY_005844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO5704242.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
BASE COUNT          207 a          184 c          127 g          189 t
ORIGIN      
        1 agtgtttccg ggaaacgata tttcttaagt ttattctcgt ctcccgacat cgttttattt
       61 cctgtcagag gccaacgacg ctcataccca cagcaataaa tacagagatg atggcaaagc
      121 cgaccaggaa cttgacgttt ttggtcatca tgtacatgac cgcaccgacc agcacctcag
      181 ccagaacaac ccatttaacg atactggagt ccttaccgaa ggtcgcctta accgtatcgt
      241 tacctttagc catcaggtcg cctttaccag cagccatagc taactcaggg aacaacatca
      301 tcagaacagc aaccgggatc actgcacgaa ccaggcgaag catattcaga cgagtgaatt
      361 tagaaaaaaa cgatttcttt tcaacgggcg cagaagcacc ctgaacactt aaaacagtat
      421 tcataagtta cctctattgt ggatagatta ataaacctaa agatctttaa ttatcgaatc
      481 agtttcctct gctccctcct ttattgcatc cagattataa aagtcaggaa aacggttcag
      541 atgatcacgt aacctgacta gcacctcatt agtttttgtt ctcccactac gttcccgggc
      601 acctaagagc ttgtgattag tagcatcgtc caataccagt aaaactttat tgcccggagc
      661 aggccttatt atatttctgc tcaagtctcc tcctcagcca cttagat
//
LOCUS       DAZFKM010000523          700 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFKM010000523 DAZFKM010000000
VERSION     DAZFKM010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>700
                     /locus_tag="ACHKAY_005845"
     CDS             <1..>700
                     /locus_tag="ACHKAY_005845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5704243.1"
                     /translation="NLLMENLAMVNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSK
                     HANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVT
                     GRDRETREWLGWGHAWAHETAVVRRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYV
                     RRIHEAELLDHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAE
                     GVLVHGDQPLMAWCV"
BASE COUNT          135 a          159 c          258 g          148 t
ORIGIN      
        1 ctaacctgct gatggaaaac ctcgccatgg tcaatccgaa tctcggctat tcagtggatg
       61 aggcctttct gtaccgggag taccgtaaag cccgggaagc cggtgaagag acattccggg
      121 ggttcatgtc aaaacacgcc aatgtggaaa ttggtcttgc cctgcgctct gaccgctggg
      181 cgggggctga tttctgggaa gagcagggcc gttgtatcag cctggacgat atcctgcgtc
      241 gtgctgatgt ggtgacggtg gggattgacg gcggagggct ggatgatctg ctggggatgt
      301 atgtgactgg gcgtgaccgg gagacccgcg aatggctggg ctggggccat gcctgggcgc
      361 atgaaaccgc ggtggtccga cggaagagcg aggcgtcccg gtttcaggat cttgttgcct
      421 gtggagatat gaccattgtc cggcgtgtcg gggatgacac ggcggaagtg gcggaatatg
      481 tgcgtcgcat tcatgaggct gagttactgg accatatcgg tattgacccg tcaggggtgg
      541 ggcagattct ggattcactg gcggaagccg ggatccccga cggaattgtg gtggggataa
      601 gccagggctg gaaactgggc ggggccatta aaaccaccga gcgcaaactg gctgaagggg
      661 tgctggtgca tggtgaccag cccctgatgg cctggtgtgt
//
LOCUS       DAZFKM010000524          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFKM010000524 DAZFKM010000000
VERSION     DAZFKM010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(312..>699)
                     /locus_tag="ACHKAY_005846"
     CDS             complement(312..>699)
                     /locus_tag="ACHKAY_005846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO5704244.1"
                     /translation="PLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAVRR
                     AEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFALDM
                     AATLVVPFTGDGRDAAEILRTLPPLK"
BASE COUNT          144 a          199 c          182 g          174 t
ORIGIN      
        1 cgtcatagca gactggccgg tcaaccgggt cagcgaactg cttccgtggc gcatagcact
       61 gccagctgaa taacacatcc ccgtcaatac ggccctcgct gtacgcttac gtttatattc
      121 agtttcctgc agtgctgcct gtggctgagc tgccatctgc ctgtccctta cgtgagtcac
      181 cccgtaacct gatgctgagg cattgctccc ttcataaaac atggtttact cactacagct
      241 tatctacctg ctccagctta tgttatgtct gttctgctga ccactgctta tataaggaag
      301 agcgtatgct gtcacttcag gggaggcagt gtacgcagga tctctgcagc atcgcgcccg
      361 tcacctgtga aaggcactac cagcgtggca gccatatcca gcgcaaaaac tctggtataa
      421 acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
      481 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
      541 gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
      601 gtttcgtcag tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
      661 ctttccatct gcctgacata gcttatatct gtcagcgga
//
LOCUS       DAZFKM010000525          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFKM010000525 DAZFKM010000000
VERSION     DAZFKM010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..410)
                     /locus_tag="ACHKAY_005847"
     CDS             complement(<1..410)
                     /locus_tag="ACHKAY_005847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5704245.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWR"
     gene            complement(410..>696)
                     /locus_tag="ACHKAY_005848"
     CDS             complement(410..>696)
                     /locus_tag="ACHKAY_005848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704246.1"
                     /translation="SAPSVRKVRFGDGYSQRAPAGLNANLKTYSVTLSVPREEATVLE
                     SFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSAEFEQVVN"
BASE COUNT          148 a          233 c          163 g          152 t
ORIGIN      
        1 atgcgccagc ggctgatcac ctcctgctcc ggatcggcgt aactgtttcc gttgacgaag
       61 ttcaccgcat ccagaaaacg ggcgtaaacc ttacgcctga ccaccgttcc gccgaccaga
      121 ctctgcatat cttccaccat accggtgacc atgccgtgca ggttagaaac cgtcagtgtc
      181 ggacgggcag cactgccctt gccgttcagt tcaaatcccg tcccctgaat gggatacgcc
      241 tgatactgtc gcccctgcca ggtgaccggc tcaccttttt cgttctgctc attacagaaa
      301 aaataacgtt ctccaccgac ctctgtcagg tcgatttccc agagcaccac gctggccgac
      361 tgctccgcac gggtgcattc attcagtgtt tcctgccgga tatcctgcat cagttcacca
      421 cctgttcaaa ctctgcgctg aactcaacac gcagcatact gacccgcgac gaccattttg
      481 cgcaggtcac ctttatctgc cgccactcat aaggcggcgt ccacagaaag gatttccagc
      541 ccccgtgctc ttccagaaac gactccagta ccgtggcctc ctcacggggg acagaaagcg
      601 tcacgctgta cgttttcagg ttggcattca gcccggcagg cgctcgctgg gaatagccat
      661 caccaaagcg cacctttctt acagaagggg ccgaag
//
LOCUS       DAZFKM010000526          696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFKM010000526 DAZFKM010000000
VERSION     DAZFKM010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..410)
                     /locus_tag="ACHKAY_005849"
     CDS             complement(<1..410)
                     /locus_tag="ACHKAY_005849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5704247.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWR"
     gene            complement(410..>696)
                     /locus_tag="ACHKAY_005850"
     CDS             complement(410..>696)
                     /locus_tag="ACHKAY_005850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704248.1"
                     /translation="SAPSVREVRFGDGYSQRVPAGLNADLKTYSVTLSVSREEATALE
                     SFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSAEFKQVVN"
BASE COUNT          150 a          235 c          166 g          145 t
ORIGIN      
        1 atgcgccagc ggctgatcac ctcctgctcc ggatcggcgt cactgtttcc gttgacgaag
       61 ttcaccgcat ccagaaaacg ggcgtaaacc ttacgccgga ccaccgttcc gccgaccaga
      121 ctctgcagat cttccgccat cccggtgacc ataccgtaca ggttagaaac cgtcagcgtg
      181 gggcgcgtac tggtgccttt gccattcagt tcaaaaccgc tcccctgaat gggatacggc
      241 tgatactgtc gcccctgcca ggtgaccggc tcaccttttt cgttctgctc attacagaaa
      301 aaataacgtt caccaccgac ctctgtcaga tcgatttccc agagcaccac gctggccgac
      361 tgctccgcac gggtgcattc attcagtgtt tcctgccgga tatcctgcat cagttcacca
      421 cctgtttaaa ctctgcgctg aactcaacac gcaacatact gacctgcgac gaccattttg
      481 cgcaggtcac ctttatctgc ctgtaaccat aaggcggcgt ccacagaaag gccttccagc
      541 ccccgtgctc agccagaaac gactccagcg ccgtggcctc ctcacgggag acagacagcg
      601 tcacgctgta cgttttcagg tcagcgttca gcccggcagg cacacgctgg gaatagccat
      661 caccaaagcg cacctccctg acggaagggg ccgatg
//
LOCUS       DAZFKM010000527          694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFKM010000527 DAZFKM010000000
VERSION     DAZFKM010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          222 a          164 c          130 g          178 t
ORIGIN      
        1 tctctttagc aaacgccatt aacaattgcc tttccattta gttaatcgtt ttgttttctt
       61 taagattagt cctattagac aaatagcaaa atggaataac tacagcacta agacgttccg
      121 ctatatagct attacctgtt cccctgacat aaggagcaga tatacctcaa ccccctattc
      181 cccagtgctg accgggagcg gcggcggtat aaaaaggaac aaggaacaaa tggcagaaca
      241 agcaaccatg tttgtccccc ccgcataacc gtaccattcg cattaagaaa tattcccata
      301 tctatcccgc aaaagaggac ccgtcataca tcaagtccgt tttactgggc atcagattat
      361 cgccgtaatt agctcagtga aagccgggta aacggttagc aagctcagtg gctcaaaagt
      421 gcatagcgct aagatgggcc tggttacacc tgccctttgc tgcagatcgt gttgatcggt
      481 gtgatgccgc cacacgttta agtcaatctg gaaaactcac aatgcgctct tctataaaga
      541 ggactctttg ataaagtaac ccgaaaaggg aaagctaact gatatcaatg taccacctta
      601 gtaatcatcc cttatataca gacaaaaaga agttgcctga aagccaggta tctaaactaa
      661 tcactaatgg tatattttat cagcacaata aaaa
//
LOCUS       DAZFKM010000528          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFKM010000528 DAZFKM010000000
VERSION     DAZFKM010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..>692
                     /locus_tag="ACHKAY_005851"
     CDS             81..>692
                     /locus_tag="ACHKAY_005851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO5704249.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLL"
BASE COUNT          241 a           89 c          133 g          229 t
ORIGIN      
        1 aaattaaaaa agtaatcagg gatgtaaaag tcaaactagg attgaagagc aaataatgat
       61 tatgatgaag ggtaattaaa ttggatttta aacatcttac tcaatttaaa gatataattg
      121 aactggacaa gcgccccgtt aaacttgatg aacgggaaac gtttaatgtc tcatggggta
      181 ttgatgagaa ctaccaggtt ggggctgcga tttcaattgc ttcaattctt gaaaataata
      241 aacaaaacaa atttaccttt cacataatcg ctgattactt agacaaagag tatattgaat
      301 tattatcaca attagcaacg aagtatcaaa cagtaattaa attatatcat attgattctg
      361 agccattgaa ggcgctacct caatcaaata tctggccagt atctatttat tatcgtttgc
      421 tttcatttga ttatttttct gcgcgattgg attcattatt atatcttgat gctgatatcg
      481 tctgtaaggg ttcattgaac gagttaatag cattagagtt taaagatgaa tatggggcag
      541 tggtaattga tgtagatgct atgcaaagta aaagcgctga gcgtttgtgt aatgaggatt
      601 ttaacggtag ctattttaac tctggtgtaa tgtatattaa tttacgggaa tggttaaaac
      661 aaagactaac ggaaaaattc tttgatctat ta
//
LOCUS       DAZFKM010000529          681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFKM010000529 DAZFKM010000000
VERSION     DAZFKM010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>681
                     /locus_tag="ACHKAY_005852"
     CDS             <1..>681
                     /locus_tag="ACHKAY_005852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5704250.1"
                     /translation="PPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGR
                     ITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKAR
                     AAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENA
                     LRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANR
                     VRDLLPWKV"
BASE COUNT          171 a          177 c          207 g          126 t
ORIGIN      
        1 gcccccggcg gtctggttcg cgtacagtcc ggaccggaaa ggtatccatc cacaaaatca
       61 cctggccggt tacagcggtg tgcttcaggc cgatgcttac ggtggttacc gggcgttata
      121 cgaatccggc agaataacgg aagccgcgtg tatggctcat gcccggagaa aaatccacga
      181 tgtgcatgca agagcgccca cctacatcac cacggaagcc ctgcagcgta tcggtgaact
      241 gtatgccatc gaggcagagg tccggggctg ttcagcagaa cagcgtctgg cggcaagaaa
      301 agccagagcc gcgccactga tgcagtcact gtatgactgg atacagcaac agatgaaaac
      361 actgtcgcgt cactcagata cggcaaaagc gttcgcatac ctgctgaaac agtgggatgc
      421 actgaacgtg tactgcagta atggctgggt ggaaatcgac aacaacatcg cagagaacgc
      481 cttacgggga gtggccgtag gccggaaaaa ctggatgttc gcgggttccg acagcggtgg
      541 tgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca atgtggagcc
      601 agaaaagtgg ctgcgttacg tcattgaaca tatccaggac tggccggcaa accgggtacg
      661 cgatctgttg ccctggaaag t
//
LOCUS       DAZFKM010000530          678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFKM010000530 DAZFKM010000000
VERSION     DAZFKM010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..311
                     /locus_tag="ACHKAY_005853"
     CDS             <1..311
                     /locus_tag="ACHKAY_005853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704251.1"
                     /translation="IRAGVQYYDRQTKKKEWTNYSAVVFAKPGAQADYYRSVLVEGGI
                     VEITGENIRVDVYQGQNGQSITLELLNAKIGFAASGNGPQQQSSNQQNTPVYDDSIPF
                     "
     gene            335..628
                     /locus_tag="ACHKAY_005854"
     CDS             335..628
                     /locus_tag="ACHKAY_005854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO5704252.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
BASE COUNT          207 a          156 c          160 g          155 t
ORIGIN      
        1 acatccgtgc tggcgttcag tattacgatc gccagacaaa aaagaaagaa tggacaaact
       61 acagcgccgt tgtatttgcc aagccgggag cgcaagcgga ttactaccgt agtgttcttg
      121 ttgaaggtgg cattgtggaa attaccggag aaaacatcag ggttgatgtt tatcaggggc
      181 aaaatggtca atcaatcact cttgaattac tgaatgcaaa gattggattt gcagcttcag
      241 gaaatggccc gcagcagcaa agtagtaacc agcagaacac tcctgtatac gacgattcca
      301 tcccattctg atttagaaaa ataaggattt aattatgcca gcgcctctgt atggtgcgga
      361 tgacccacgc cgctgttccg gcaattccgt atcggaggtg ctggataaat tcagaaaaaa
      421 ctacgatcgg ataatgtcgc taccgcagga aacgaaagag gaaaaggaat ttcgccattg
      481 tatatggctt gcagagaaag aagaacgcga gcgaatttac cagacatcaa tccgaccatt
      541 ccgcaaagcc acatataccc acttccctga aattgacccg cgcctgcgta attaccgctc
      601 acgctatggc gctatcagta atgactgagg aatttaccat gagaggactt gcatacaatc
      661 ccggcattct tccggcag
//
LOCUS       DAZFKM010000531          668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFKM010000531 DAZFKM010000000
VERSION     DAZFKM010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>668)
                     /locus_tag="ACHKAY_005855"
     CDS             complement(<1..>668)
                     /locus_tag="ACHKAY_005855"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO5704253.1"
                     /translation="FKYVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARS
                     QNYALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVN
                     SENVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLK
                     NIDEYINNNSEDKLIVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINT
                     YDNT"
BASE COUNT          202 a          133 c          106 g          227 t
ORIGIN      
        1 tgtattatca taggtattaa tcaattgctc atgcgtacaa ttttcgatat cactacgatc
       61 acatgttggt ttaaaatatt tatgtggctc aggataacgg agatagtagg taggaccatg
      121 actacccatc aaatgaaata cgataagttt atcctctgag ttattattta tatactcatc
      181 aatatttttt agcataacct catcataaca tgagtttttc ttgcataatg tattatctga
      241 atttgatggc tcaatattaa ttgtagggat tcgcttacat acacctttac atcccccgtc
      301 attttcaatc cagagtaaat ttacccccgt tctgtacaga atatccagta cattttccga
      361 atttaccgct ttgcgggaat tgaactcttt tcgcttcata tctgaaaaca tgcatggaac
      421 agatatggct gtggatgtgc cacacgactg cacattatga aaggatataa gttcattata
      481 tttctttgta aaatcattgg taccacgaga atatccattc agagcatagt tttgacttct
      541 tgctgtctcc ccgacaacta aaaacataat taccggcttt tgtttttcat ttattgtgcg
      601 ggatgcatcc gttccagtct gacgaaactc gccaggagaa acaaaaaaat cccttacata
      661 tttaaacc
//
LOCUS       DAZFKM010000532          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFKM010000532 DAZFKM010000000
VERSION     DAZFKM010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(280..>661)
                     /locus_tag="ACHKAY_005856"
     CDS             complement(280..>661)
                     /locus_tag="ACHKAY_005856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO5704254.1"
                     /translation="SWAVNHYRDNAIAYKEQRDKATSIIADMQKRQRDVAELDARYTK
                     ELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSMGDGESPGLTSDAELNYYRL
                     RGGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT          149 a          165 c          162 g          185 t
ORIGIN      
        1 cttctgctgc ggtaactaac tggcgaaagt cgaagtcatg attcgcaatt aattcattca
       61 tggcgttgcc tgcttctttg aaatgaacct ttgccgcaca ggaaaccagc ccaccgaggc
      121 tcgccagcac taactggtat cctcaaaggc ccattccaaa gggtcaggtt cggtgtttat
      181 tgtgcgctgc ggtgcgcggt gaaataccgg tacaaaaatg ccccgcatct gcgaggcatt
      241 ttcctgaaag tcacttgtta aatttcagtg aaattaaaat tattttaagc actgcgtcct
      301 gatgtattcc tgcaggtagt taacctgcgc ggttatcttg tcgattccac ctcggagacg
      361 gtaataattg agttcagcat ctgatgtaag tcctgggctt tctccatcgc ccatgctgct
      421 ggctccggtc gttgactttg cacaggtggc ggagacttgc aggcgcttac gcccagcaga
      481 aacatcagca cggagacttt cgatagtcgc attagcatca gcaagctcct ttgtgtatct
      541 ggcgtcaagt tctgctacat cacgttgccg cttctgcata tcagcgatga tggatgtggc
      601 tttatcgcgc tgctctttgt aggcgatggc gttatcacgg taatgattaa cagcccatga
      661 c
//
LOCUS       DAZFKM010000533          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFKM010000533 DAZFKM010000000
VERSION     DAZFKM010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>659
                     /locus_tag="ACHKAY_005857"
     CDS             <1..>659
                     /locus_tag="ACHKAY_005857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO5704255.1"
                     /translation="QAAHALAIFKQLRIVDAPGSPTFGESCAQWVFDLVAALFGSYDA
                     QTGVRHIKEVFILIPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPTVEVAANAFNP
                     ARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVVAADPNTVSGIKSVGTLIDELWL
                     FGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARDVRDGKIHD
                     P"
BASE COUNT          143 a          181 c          203 g          132 t
ORIGIN      
        1 caggccgcac atgccctggc gatttttaaa caactgcgga ttgtggatgc accgggcagc
       61 ccgacgttcg gggagtcctg cgcacagtgg gtgtttgacc tggtggcggc cctgtttggc
      121 tcctacgatg cgcagaccgg tgtacgccat atcaaggaag tttttatcct tatccccaag
      181 aaaaacagca agtccacgct ggctgccggg atcatgatga cggcgctgtt actgaactgg
      241 cggcaggcgg cgggctacac cattctggcc ccgaccgtgg aggtggcggc taacgccttc
      301 aaccctgcca gggatatggt acgacgggac gatgatctgg atgacctctg tcaggtgcag
      361 acacatatcc ggaccatcac ccacagggtg acggacacca cccttaaggt ggtggctgcc
      421 gatccgaata cggtatccgg tatcaagtcc gtggggacgc tgattgatga actgtggtta
      481 tttggcaagc agtacaaagc ggaggacatg ctacgtgaag ccataggcgg ccttgcctcc
      541 cgcccggaag ggtttgtggt gtatacgacc acccagtcga atgagccgcc agccggggtg
      601 ttcagacaga aactgcagta cgcccgggat gtccgtgacg gcaaaattca tgatccgca
//
LOCUS       DAZFKM010000534          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFKM010000534 DAZFKM010000000
VERSION     DAZFKM010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>659
                     /locus_tag="ACHKAY_005858"
     CDS             <1..>659
                     /locus_tag="ACHKAY_005858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704256.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     L"
BASE COUNT          149 a          181 c          204 g          125 t
ORIGIN      
        1 ccgcgcccgt ttaatatccg gatgcgcagg atgacgccgg acagcaccac agaccagctg
       61 cagaacaaaa cgctctggtc gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg
      121 aacacggcac tggtcggcgt acaggtggac tcggagcagt tcggcagcca gcaggtgagt
      181 cgtaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
      241 cgacaataca gcggtatctg ggacggaacg ttaaaaccag catacagcaa caacatggcc
      301 tggtgtctgt gggatatgct gacccacccg cgctacggca tggggaaacg tcttggtgcg
      361 gcggatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgccg
      421 gacggctttg gcggcacgga gctgcgcatc acctgtaatg cgtacctgac cacacagcgc
      481 aaggcgtggg atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
      541 gggcagacgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
      601 agtaatgtgg tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaa
//
LOCUS       DAZFKM010000535          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFKM010000535 DAZFKM010000000
VERSION     DAZFKM010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>656
                     /locus_tag="ACHKAY_005859"
     CDS             <1..>656
                     /locus_tag="ACHKAY_005859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5704257.1"
                     /translation="KALAAGATEITKEEYFARKNGTYSGSQQNTGASDTTAQPEPVKV
                     TADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQNQHE
                     SERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVMGDA
                     TYQETFDEEYQVEVQEDDPEKMEGAEHPHKENTGGNQHHASDNETGETADHSIKVNGH
                     "
BASE COUNT          218 a          160 c          174 g          104 t
ORIGIN      
        1 aaggcactgg ctgccggggc tactgaaatc acgaaagaag aatatttcgc ccgcaaaaac
       61 ggtacatact caggttcaca acaaaatact ggtgcatctg acacgaccgc acaaccagag
      121 ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc aggcagccaa tatcagccag
      181 cctgacgccg ataagttgct tgctgcctct cgcggagaat ttgttgcagg gattagcgac
      241 ccgaatgatc cgaaatgggt taaggggatc cagacccgcg attctgtaaa ccagaaccag
      301 catgaatcgg aacggaacta ccaaaaagcg gaacaaaaca gcccaaatgc gttacaaaac
      361 gagccagaaa cgaaacagcc tgaaccagtg gcgcaacagg aagtggaaaa agtctgcacc
      421 gcctgcggtc agaccggcgg cggcaactgc cctgattgtg gcgcggtgat gggcgacgca
      481 acataccagg aaacattcga tgaagagtat caggttgaag ttcaggaaga tgatccggag
      541 aaaatggaag gcgctgaaca tccacacaag gagaacactg gcggcaatca gcatcatgcc
      601 agcgataatg aaactggcga gacggcagat cactcaatta aggtgaacgg tcatca
//
LOCUS       DAZFKM010000536          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFKM010000536 DAZFKM010000000
VERSION     DAZFKM010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>653)
                     /gene="espL2"
                     /locus_tag="ACHKAY_005860"
     CDS             complement(<1..>653)
                     /gene="espL2"
                     /locus_tag="ACHKAY_005860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO5704258.1"
                     /translation="NKEELIHILSARTLDGTIPGLYQALQNGHAQAIKSYGNLVLDTI
                     DKNIDLEYLLSAFKYEAHSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSNLKRGEIIR
                     MLEARNYDGAPGLLLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSL
                     AISHRHDHVVKLYGKLFKKLDTSPYKMSIILALAIDCERNNANIIIDSEYKSNKAVK"
BASE COUNT          208 a          123 c           87 g          235 t
ORIGIN      
        1 cctttactgc cttatttgac ttgtactcac tatcaataat tatgtttgca ttatttctct
       61 cgcaatcaat tgctaatgct aggataattg acattttata tggactggta tcaagttttt
      121 taaataactt tccgtataat ttgacaacat gatcatgtct atgactgatc gctaatgata
      181 accccgtaaa atcataatgt tttgcagtca ataactcttc gataaaatcc tttgatatat
      241 ctaacattat tagtgagtca aaaaaagact gaatcgtatt aatatctcca ttttgatacg
      301 caagaagtaa tcctggtgca ccatcataat ttctcgcctc aagcatcctg attatctcac
      361 ctcttttcaa attactattg ccaagtacgc cacaatatgc ctttattgcg tctgcatgac
      421 cattttgaaa tgcagaaaat aatccaggag tatatttatt agagctatgt gcctcatatt
      481 taaatgctga aagcaaatac tctaaatcta tatttttatc aatagtatcc agaactaagt
      541 taccataact cttaatagct tgagcatgtc cattttgaag ggcctgatat aaaccaggta
      601 tggttccatc caaagttctt gcagataata tatgtattaa ttcttcttta tta
//
LOCUS       DAZFKM010000537          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFKM010000537 DAZFKM010000000
VERSION     DAZFKM010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>650)
                     /locus_tag="ACHKAY_005861"
     CDS             complement(<1..>650)
                     /locus_tag="ACHKAY_005861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO5704259.1"
                     /translation="KNWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQ
                     FSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIE
                     MLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVF
                     IHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQK"
BASE COUNT          201 a          123 c          101 g          225 t
ORIGIN      
        1 gcttttgttt aaatttatgt tttgcacaat accgatactg attgctattt acaggtttaa
       61 gtaaatcttc tttactccac ggagaagctg ctttggcgat caaaaaacta cgtgacaccg
      121 gatagtcagc ccactcatgc cagggttttg taggtcccac atagtgtata aacacggtgt
      181 catcattgac tggattatca actttgtctt ttaattcata gttaatacta tatcgggtat
      241 tatatttctc cgaaataaac ttcactttac cattcaataa tacattcagt acatcttgat
      301 caaggtgggt gattttactt acccaatctg ggtcacgcaa catttcaata gcttttgacg
      361 aaatgttatt tagattccac tcatcaatat ttatcagtaa aaaaccagca ttgaaatatc
      421 cagaagctaa ctgtggtgta gttaataccg aggctcgatt ttgccaccat tcaacatctc
      481 tttcagctac aactgcggca atttcattag tagaaaattg ataatctaag agttctttaa
      541 tactaccctt gcaagcaata tctgcatcaa gatatagtat tttttcatgt ttatgataga
      601 aataatctgc aattataaat cgaaaatatg ttgcgtaagt ccagtttttc
//
LOCUS       DAZFKM010000538          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFKM010000538 DAZFKM010000000
VERSION     DAZFKM010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..456
                     /locus_tag="ACHKAY_005862"
     CDS             <1..456
                     /locus_tag="ACHKAY_005862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO5704260.1"
                     /translation="LVDRWRELEGATAKIPQTFSEALRLAADLEDQKAELEKQLALAA
                     PKVEFADRVGEASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGARRNVPMQEYMDR
                     GYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            456..>649
                     /locus_tag="ACHKAY_005863"
     CDS             456..>649
                     /locus_tag="ACHKAY_005863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO5704261.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDK"
BASE COUNT          213 a          138 c          174 g          124 t
ORIGIN      
        1 cttgttgacc gctggcgaga actcgaaggg gcaaccgcga aaataccaca aaccttttct
       61 gaggcattgc gccttgcggc cgaccttgaa gaccagaagg ctgaactgga gaaacagctt
      121 gctctcgcag cacctaaagt tgagtttgcc gatcgagttg gcgaggccag cggaattttg
      181 attggaaact ttgcaaaggt tgttggtatt ggtccaaaca aactgtttgc gtggatgcgc
      241 gatcacaaaa tccttattgc ttcaggtgcc cggcgcaatg tgccaatgca ggaatatatg
      301 gatcgcggct atttcacagt gaaagaaaca gcggtcaata caaatcacgg aatacagata
      361 tcgttcacca caaaaatcac cgggcgtggt caacagtggc tgacaagaaa gctgctagat
      421 aacggaatgc ttaaagtaac aggggaggct gcttaatggc taaaccagcg cgaaggaaat
      481 gcaaaatctg taaggaatgg tttcacccgg cattctcaaa tcagtggtgg tgcagcccgg
      541 aacacggaac taaattagcg ctcgaacgac gaaataaaga acgcgaaaag gcggaaaaaa
      601 cagcagagaa gaaacgacga cgagaggagc agaaacagaa agataaaat
//
LOCUS       DAZFKM010000539          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFKM010000539 DAZFKM010000000
VERSION     DAZFKM010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..369
                     /locus_tag="ACHKAY_005864"
     CDS             <1..369
                     /locus_tag="ACHKAY_005864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704262.1"
                     /translation="AVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPE
                     AIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIGPEAAGSLVQLI
                     DQRVQAVMLSMRRDGGMLSG"
     gene            362..>648
                     /locus_tag="ACHKAY_005865"
     CDS             362..>648
                     /locus_tag="ACHKAY_005865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704263.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVT"
BASE COUNT          126 a          151 c          239 g          132 t
ORIGIN      
        1 gcggtggcca atgcaaaagg cggcgtgtat acctcggcaa acctgagtgc gtacagcaac
       61 agtattgtgg atacgcccac gtactttgcc tttgcaaaag gggccgggct gatgggggag
      121 gccggacctg aagccattat gcctctgacg cgggcggcgg atggctcgct gggtgtgcgc
      181 gcggtgggta gtatgaacgg cagtgcgggt ctggtgtatt ccccggtcta ccacatcgcc
      241 attcagaatg acggggctaa cggacagata gggccggaag ctgcgggcag ccttgtgcag
      301 ctgattgacc agcgggtgca ggcggtgatg ctgtccatgc gacgtgacgg aggaatgctg
      361 agtggctgag ataaaaacgc tgcatctggt cccgcgtgaa gggatgcagg tgagtgagaa
      421 gccgtcggtg gtgagggtgc ggtttggtga cggttatgaa cagcgccgcc ccacagggct
      481 gaatcctcaa ctgaagacgt ttcaggcggt gttccgggtg acggatgagt caacccggcg
      541 ctggctggat gaatttttat cctggcatgg tggttaccgt gcctttttgt ggcgaccgcc
      601 gaaacataac cggacggtga gggtggtgtg ccgggagtgg agcgtcac
//
LOCUS       DAZFKM010000540          632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFKM010000540 DAZFKM010000000
VERSION     DAZFKM010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..521
                     /locus_tag="ACHKAY_005866"
     CDS             222..521
                     /locus_tag="ACHKAY_005866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704264.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            557..>632
                     /locus_tag="ACHKAY_005867"
     CDS             557..>632
                     /locus_tag="ACHKAY_005867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704265.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDIND"
BASE COUNT          205 a          124 c          147 g          156 t
ORIGIN      
        1 cattgtagtc accactcaaa gcggcatgac cattcagatt cataaaccaa aagaggaaaa
       61 atgatgtggc ctatatgtgt taattgcgga cggatgtgcc tatctggatg gtgccgaaag
      121 tgcgacaaat gcacgaagaa aagacaataa caatcctcgc actgacgggg atttctttta
      181 tctgaactcg ctacggcgag ttttgtttta tggagatgat tatggcctgt tcaacattca
      241 accctttaac gttacagaaa taccagccag accctgaaga tttatgctca ctgtgtggcg
      301 gaaatcatgg taaagctgcc atgatcgaat gtaaggacaa aatccacata tgccttaatt
      361 gcgttgatgt cctcgttgat atcaaaaatg aaagagaaga taaaaagcgt agcgaggcta
      421 ttcgcgcctt agattcatgg atgcgagatg ggtatagtgc tgcgcaaatt tatgacttag
      481 ccatttcaaa aggcgaaata ccaggtgtgc gaatcgaata agaagcgcac tcaagcatct
      541 tttggggaaa tcacaaatgc acttccgagt cacaggtgaa ttgaatggag aaccattcaa
      601 cagagttatc gaagcagagg acatcaacga ct
//
LOCUS       DAZFKM010000541          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFKM010000541 DAZFKM010000000
VERSION     DAZFKM010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..628
                     /locus_tag="ACHKAY_005868"
                     /pseudo
     CDS             1..628
                     /locus_tag="ACHKAY_005868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          207 a           88 c          119 g          214 t
ORIGIN      
        1 catgcaataa atttgtttcg tgaagtctca atcagtaatg atactataag tgtaaaattc
       61 tacagaaatg aaaaaaattg aatgcgcttg caattttctg atggataaag atgcgcaggg
      121 gtatatcgac ctgtctgatt tggatttaac aagttgtcat tttaaaggtg acgttatatc
      181 gaaggtgtct tttttatcat caaatctaca acatgtaaca ttcgaatgta aagaaattgg
      241 ggattgcaat tttactactg caatagttga taatgtcata tttagatgtc gacgtttaca
      301 caatgtgatt tttatcaaag cgagtggtga atgtgtcgat ttcagcaaaa atattcttga
      361 tacagttgac ttctcgcaga gtcaacttac tcagagtaat tttcgcgaat gtcagattag
      421 aaattcaaac ttcgataatt gttatcttta cgcttcgcac ttcaccagag cagagtttct
      481 gtctgccaaa gaagtatcat ttattaaatc gaatatgaca gctgttatgt ttgatcatgt
      541 gcgaatatcg acagggaatt ttaaagattg cattacagaa caattggaat taactattga
      601 ttattcagat atatttggga atgaagat
//
LOCUS       DAZFKM010000542          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFKM010000542 DAZFKM010000000
VERSION     DAZFKM010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..498
                     /locus_tag="ACHKAY_005869"
     CDS             <1..498
                     /locus_tag="ACHKAY_005869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704266.1"
                     /translation="ANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSY
                     ASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEI
                     FHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTA
                     GISIS"
BASE COUNT          162 a          145 c          171 g          143 t
ORIGIN      
        1 gcgaataccg gcacgctcaa taatgtcacc attaacgaga actgtgtcat cagagggaaa
       61 ctgtctgcaa accagattga aggcgacctg gtgaagacgg tggggaaagc ctttccccgg
      121 aataacagtt atgccagcgg gacggtaacc gtcacagttt acgatgacca gggattcgac
      181 cggcagatta tcattccccc ggtgctgttt cgcgggacga aacaccagaa tttcaacagc
      241 ccgaatcagc agtcgtactg gtattccacc tgtaagctgc aggtgctgaa gaacggggtt
      301 gagattttcc atgaaccggc aacggatgtc agccgggtgt tctcatcggt gatagatatg
      361 ccggcagggc ggggtcatgt caccctgacg tttaatgtgt cgtcggccgg tgcgaacaac
      421 tggacgccga caacgtacat cagtgattta ctggttgtgg tcatgaaaaa atccacggca
      481 gggatcagta tcagctgacg gtttattaac ccggacgggc acctcaggag gtgccttttt
      541 tattgactga aaacaaagag gtaatcatgc ggcatttata cgcaacgata ttattgttta
      601 ctaccctgct ggcaggaatt g
//
LOCUS       DAZFKM010000543          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFKM010000543 DAZFKM010000000
VERSION     DAZFKM010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>615
                     /locus_tag="ACHKAY_005870"
     CDS             <1..>615
                     /locus_tag="ACHKAY_005870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO5704267.1"
                     /translation="LHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKKEIQRVYDENHQ
                     VYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNR
                     QFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQ
                     ALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDS"
BASE COUNT          143 a          150 c          184 g          138 t
ORIGIN      
        1 aactgcatat tgctccgtca acgtattacc attgtcagca acagcgacat catccggata
       61 aacgcagtgc ccgtgcgcag cacgatgact ggctgaagaa agagatacag cgcgtatacg
      121 atgaaaatca ccaggtatac ggtgtgcgta aagtctggcg tcagttgtta cgggaaggta
      181 tcagagtggc cagatgcact gtggcacgtc tcatggcagt tatgggactt gccggtgttc
      241 tccggggtaa aaaggtccgt acgaccatca gccggaaagc cgttgccgca ggcgaccgcg
      301 taaaccgtca gttcgtggca gaacgacctg accagctgtg ggtggctgat tttacttacg
      361 tcagcacatg gcagggcttc gtctatgtgg cgttcatcat tgatgtgttt gccggataca
      421 ttgtggggtg gcgggtctca tcgtccatgg agacgacatt cgtgctggat gcactggagc
      481 aggcgttatg ggcccgtcga ccgtccggca cggtccatca cagtgataaa ggttctcagt
      541 atgtatcgct ggcctacaca cagcggctta aggaagccgg attactggca tcaacaggaa
      601 gtacaggcga ctcgt
//
LOCUS       DAZFKM010000544          613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFKM010000544 DAZFKM010000000
VERSION     DAZFKM010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..>613
                     /locus_tag="ACHKAY_005871"
     CDS             89..>613
                     /locus_tag="ACHKAY_005871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO5704268.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNF"
BASE COUNT          182 a          117 c          142 g          172 t
ORIGIN      
        1 atttaaatgg cggtcaatta ttgttattat taagtctaat gtcatttaaa ggcattatca
       61 tatttacgaa gagatcagga tgatgacgat gaaaaaaagt gtattgacgg cgtttataac
      121 tgtggtatgt gcaacgtcca gcgttatggc agctgatgat aatgctatca cggatggcaa
      181 agtaacattt aatggtaaag ttattgctcc agcttgtacc ctggtagctg cgaagaaaga
      241 ttcagtagtc acattgccaa atgtcagcgc caccaagttg caaactaatg gagcagtctc
      301 tggcgttaaa actgatgtac caattgcctt agaaggctgt gatgttactg taactaaaaa
      361 tgcgacgttt acctttagtg gtacggctga cggcgtgcaa cctacagcat ttgccaacca
      421 ggctaccacc gatgcagcga cgaatgttgc tctacaaatg tatctgccag atggttctac
      481 atcggtaact cccggtaccg aaacgtcgaa tattcagtta gcggattctg ctgagcaaac
      541 agtgaccttt aaagttgatt atatcgctac ggggaaagcg acttctggta atgtgaatgc
      601 ggtaacaaat ttc
//
LOCUS       DAZFKM010000545          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFKM010000545 DAZFKM010000000
VERSION     DAZFKM010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>611
                     /locus_tag="ACHKAY_005872"
     CDS             <1..>611
                     /locus_tag="ACHKAY_005872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704269.1"
                     /translation="AQQPALFTAMLKQFRADLTVFNAQCHGGSAVNVPWICGDTTYYW
                     KNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPDIPASGYYGAAS
                     RTNGNQVSSNRPTHFSSWARRSIIPDRMATAILNAAGRTSAFISGKAPEIKPSPGGDT
                     PSGPSADTSVRTISLLPTAGEAAAQGWTIKDGGIQLSGGVFKI"
BASE COUNT          147 a          162 c          172 g          130 t
ORIGIN      
        1 acgcacagca acctgcgctg tttacagcca tgctgaagca gtttcgtgct gacctcactg
       61 tgtttaacgc gcagtgtcat ggtggcagtg ctgtaaatgt gccgtggatt tgtggtgaca
      121 cgacgtatta ctggaaaaat acatacgcta cccagtacga caccgtgtac ggcgggtata
      181 aaaacaggga gagtgagggc gtttattttg tgcccttcat gacagacggt aacggcgtca
      241 ataccgccac taacgcaccg gcagaagatc cggatattcc ggcatcagga tattacggtg
      301 cggcatcgag aacgaatgga aaccaggtat catcaaaccg cccgacacat ttcagttcat
      361 gggcgcgcag gagcattatt ccggatcgta tggcaaccgc tattctgaac gcagccgggc
      421 gcacctcagc tttcatcagt ggtaaggcac cggaaatcaa accctcgccc ggcggcgaca
      481 cgccatcggg tccgtctgca gatacgtccg ttcgcacaat ctccctgctg ccgacagccg
      541 gagaggctgc tgcgcagggc tggaccatta aggacggcgg aattcagttg tcgggtggtg
      601 tatttaagat c
//
LOCUS       DAZFKM010000546          607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFKM010000546 DAZFKM010000000
VERSION     DAZFKM010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..429
                     /locus_tag="ACHKAY_005873"
     CDS             <1..429
                     /locus_tag="ACHKAY_005873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO5704270.1"
                     /translation="AMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQ
                     KAAQWLQACDGNRVQEYVKLERLRNALSGNSPVTPDGWICCSERMPDKLIPVMVMYED
                     GEMWSAMWNGNRWDDGTEYPDPHSVTHWREMPAAPQQEVK"
     gene            429..>607
                     /locus_tag="ACHKAY_005874"
     CDS             429..>607
                     /locus_tag="ACHKAY_005874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO5704271.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFY"
BASE COUNT          156 a          125 c          176 g          150 t
ORIGIN      
        1 gccatgcttc atggtgccga acctgtaagc caaacttaca agtcacaaca tacgcagttt
       61 gaacaagttg ctgacctcta cgaaatgcaa tttgatgacg gtcgcacttg tgcctttcac
      121 actgatgcgc aaaaggctgc gcaatggctt caggcatgcg acggaaacag ggttcaggaa
      181 tacgtgaagc tggagcgatt gcgtaatgcg ctatcgggca actctccggt aactccggat
      241 ggttggatat gctgtagtga gcgaatgccg gataagttaa ttccggtaat ggtcatgtat
      301 gaagacggtg agatgtggtc tgcaatgtgg aatggcaatc gctgggatga tggcaccgaa
      361 tatccggatc cgcactcagt tacgcactgg cgtgaaatgc cagcagcacc gcagcaggag
      421 gtgaagtgat ggactccttc gcgaaatata cgattattga ctggatagca ttccttcagg
      481 ttttgctcat ctggttttat atggcttaca ggagtgggca gtggattgtc agtgtagcct
      541 gtagcaaggg atggcgttgg tggaaccgaa agaataaaaa agcgctggcc ttggattcgt
      601 tttacga
//
LOCUS       DAZFKM010000547          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFKM010000547 DAZFKM010000000
VERSION     DAZFKM010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>604
                     /locus_tag="ACHKAY_005875"
     CDS             <1..>604
                     /locus_tag="ACHKAY_005875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704272.1"
                     /translation="RHAEEAKKNAGEAETSARNAGISASKAEASAANADTSAEDASES
                     ARQAAESAASAKKSEEASSSSASEAAQKASESLQSATDAELSKKTAESAAGNASRDAT
                     TATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGDPGPAGPQGPKGDKGER
                     GDTGPAGATGERGPAGDAGPAGPQGPKGDRGERGETGLTGS"
BASE COUNT          171 a          154 c          219 g           60 t
ORIGIN      
        1 gcgtcacgca gaggaggcga agaagaatgc cggagaagca gagacgtccg cgaggaatgc
       61 cggcatatca gccagtaagg cggaagcgag cgccgcaaat gctgatactt cagcagagga
      121 tgcatcggag tcagcccggc aggcggcaga aagtgcagcc tctgcaaaga agtcagagga
      181 agcgtcctcg tcctcagcct ctgaggccgc tcaaaaagcc agtgagtcat tacaaagtgc
      241 aacagatgcc gagttgtcaa aaaagacggc agaaagtgca gccggtaatg catccagaga
      301 cgcaacgaca gcaacagaaa aagcccggga gtcagcagaa agcgcacagt cagcggaaca
      361 aagcaggata gcggcggaag aagccgtaaa ccgaatcccc accgtggtgg ggccacccgg
      421 gccaaaggga gatccggggc ccgcgggtcc tcaggggccg aagggagata aaggagagcg
      481 tggcgacacc ggcccggcag gggcaactgg tgaacggggg ccggcaggtg atgctggtcc
      541 ggcaggcccg caggggccga aaggcgacag gggggaacgg ggagagaccg gtctgacggg
      601 aagt
//
LOCUS       DAZFKM010000548          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFKM010000548 DAZFKM010000000
VERSION     DAZFKM010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..136)
                     /locus_tag="ACHKAY_005876"
     CDS             complement(<1..136)
                     /locus_tag="ACHKAY_005876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO5704273.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     A"
     gene            525..>601
                     /locus_tag="ACHKAY_005877"
     CDS             525..>601
                     /locus_tag="ACHKAY_005877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704274.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQI"
BASE COUNT          203 a          156 c          110 g          132 t
ORIGIN      
        1 cggcggcaga gtcataaagc acttcattac ccttgccacc accccgcaga acgggcattc
       61 cctgctcctg ccagttctga atggtacgga tactcgcgcc gaaaatgtca gccagctgct
      121 ttttgttgac ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc
      181 caaaaagctc gttttcagca cctgtcgttt cctttctttt cagagggtgt tttaaataaa
      241 aacattaagt tacgacgaag aagaacggaa acgccttaaa ccggaaaatt ttcataaata
      301 gcgaaaaccc gcgaggtcgc cgccccgtaa cctgtcggat cgccggaaag gacctgcaaa
      361 atgataataa ttatcatcta tatgtcacaa cgtgcatcta cgccatcaaa ccacgtcaaa
      421 taatcaatta tgacgcaggt atcgtattaa ttgatctgca tcaacttaac gtaaaaacaa
      481 cttcagacaa tacaaatcag cgacactgaa tacggggcaa cctcatgtca acgaagaaca
      541 aaacccgcag aacaacaacc cgcaacatcc gctttcctaa ccaaatgatt gaacaaatta
      601 a
//
LOCUS       DAZFKM010000549          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFKM010000549 DAZFKM010000000
VERSION     DAZFKM010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            243..>597
                     /locus_tag="ACHKAY_005878"
     CDS             243..>597
                     /locus_tag="ACHKAY_005878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704275.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDGKFQLKIEDLQGSDKLANFNFFLIKKSNLS
                     GLYMYHHGSCSLNTLF"
BASE COUNT          203 a          116 c           98 g          180 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg taaattccag cttaaaattg
      481 aagatcttca aggaagtgac aaacttgcta atttcaactt tttcttaatt aaaaaatcta
      541 atctttctgg tctctatatg tatcaccacg gttcatgcag tctgaacact ctctttt
//
LOCUS       DAZFKM010000550          590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFKM010000550 DAZFKM010000000
VERSION     DAZFKM010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..413
                     /locus_tag="ACHKAY_005879"
     CDS             48..413
                     /locus_tag="ACHKAY_005879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5704276.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELEHRIALQFGGGNEETNLWTLCTECHRQKSAREAASGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          125 a          141 c          177 g          147 t
ORIGIN      
        1 tgacattttc tttcctctgg tgacaagtta tatggcgagg attttacatg gctgtgcttc
       61 gtacgttacc gggcagaatc aaaactctga acacccggcg ggtgaatgtc ctgaagggtg
      121 aacagcgtcg ggtcagtggt agtgcccggg tttccctcaa gcgtcgtatc tggctgaggg
      181 atgccggaca gtgctgtctc tgtgggcgtg tggttgacct ttgtgacagt gaacttgagc
      241 accgcattgc acttcagttc ggtggtggta atgaggagac gaatctctgg acgctctgta
      301 ccgaatgcca tcgacaaaag tctgctcgtg aagcggcgag tggtatgcca gacccgacgc
      361 tgccggaggt gtccggaggt catggcaggg cagacgatat catcggactg tgacccgccc
      421 cggggggggg gatcatccgg cgaaaaaaac gatcgccccg gacaccgccc cccctctcac
      481 gcagagaaaa aattcccgtt tcagggcagt taacatgtta actggctgtc cgggcatttt
      541 tgcggttttt atctttatta ttcagtttgt tgtgcggaaa aaatgttaac
//
LOCUS       DAZFKM010000551          589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFKM010000551 DAZFKM010000000
VERSION     DAZFKM010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..126
                     /locus_tag="ACHKAY_005880"
     CDS             <1..126
                     /locus_tag="ACHKAY_005880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704277.1"
                     /translation="RCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKGLL"
     gene            283..>589
                     /locus_tag="ACHKAY_005881"
     CDS             283..>589
                     /locus_tag="ACHKAY_005881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO5704278.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKYEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKTAAKKLNNKIINKFGCEIGLGANIGKGLTIPHHA
                     "
BASE COUNT          184 a           97 c          127 g          181 t
ORIGIN      
        1 cgctgtgatg aggatttgcg ggagcgcctg caatcctcat cgttcagcga tatgttgtcc
       61 ggcttcaaag ataaggtgac atcatccctt aatagcgcga ccagctcagt taaggggctg
      121 ctctgattta acgtatgtcg ccaatttcct gatgaaggtg actggcgact tgctgttgta
      181 tgtccttctc agaaaattgt ttttgaataa caaacaacag gattttataa tctcttaacc
      241 ttataacatg tgtggtctga aataataatt aaggagatta tcgtgctgtc ttacttaatg
      301 gcaattcact ttgttttatt tgggaactct actaatttaa aaaacttctg gaaatatgaa
      361 gtaattcggc ggaaacgtat ggatatctgg aggcttttaa gagagaaaaa acagcgtaac
      421 cggaatttcc ttttctggtg gcggttggct aacgaaatgt atattaatgg taataaatta
      481 cataagacag cagccaaaaa gttaaacaat aaaataatta acaaatttgg ttgtgaaatt
      541 ggattgggcg caaatattgg aaaagggtta acaattcccc atcatgctg
//
LOCUS       DAZFKM010000552          589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFKM010000552 DAZFKM010000000
VERSION     DAZFKM010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..495
                     /locus_tag="ACHKAY_005882"
     CDS             <1..495
                     /locus_tag="ACHKAY_005882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO5704279.1"
                     /translation="LYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINEN
                     KEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNK
                     VTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIR
                     QGSN"
     gene            497..>589
                     /locus_tag="ACHKAY_005883"
     CDS             497..>589
                     /locus_tag="ACHKAY_005883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO5704280.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSN"
BASE COUNT          227 a           72 c          118 g          172 t
ORIGIN      
        1 ctgtatacat atccgctgag cccggtgaat agcatggatc cattaggatt atatgaattt
       61 aaatcaaaaa atatagatga tattggaata tttgcattgg caatgtgtaa tggagaatca
      121 attaacgaga ataaagaata tggtggacta atatgtaaga agcaaggtga atatttcccc
      181 atgaatccga taagttcaaa tgataatgat agtgtagact tgcgaaatat aaaatgccct
      241 gaaggttcag agagagtagg cgattatcac actcacggtt tttactctga cgataaagga
      301 aataaagtaa caaaagaaaa tgatgtttat gatagtctaa atttttcaag caaagattta
      361 acgaattctt atatgaatgg aatgggaaaa aaagaataca gtagttactt gggaacacca
      421 aataacacct atctaaaata taatcccaaa gctaaaggga atggagttac aattatcagg
      481 caagggagta attaaaatga aggcaataca atgttgttat ttccttgctt tagtctccag
      541 tcatttaatg gctggagtta tgggtgatga taatatattt ttaagtaat
//
LOCUS       DAZFKM010000553          587 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFKM010000553 DAZFKM010000000
VERSION     DAZFKM010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>587
                     /gene="rfaY"
                     /locus_tag="ACHKAY_005884"
     CDS             <1..>587
                     /gene="rfaY"
                     /locus_tag="ACHKAY_005884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO5704281.1"
                     /translation="KVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTAR
                     GPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTL
                     NFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSE
                     KGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNEL"
BASE COUNT          210 a           83 c          104 g          190 t
ORIGIN      
        1 gaaagttttc ataaaagata atgatccctt ttatgagcaa gttctaaatg actttttaac
       61 ttgcagggtt aaaacattaa aagtttttcg ttcgattgat gatactaaag tcattcttat
      121 tgacactgct cgtggacctc ttgtacttaa agtttatgcg ccaaagcata aaatgaccga
      181 acgtttctta aaatcttgca taaaaaaaga ttattatgaa aatttaattt atcaaaccga
      241 tcgggtacgt ggtgaaggca tccaatcaat taatgactat tttttgctag ctgaacgtaa
      301 aacattaaat ttcgctcatt attatattat gctcattgag tatattgaag gtgtcgggtt
      361 aaatgagtat ttggaaattt ctgaagatct gaaggatcaa ttatcagaat cgataaaaga
      421 gttgcatcag catggaatgg tgtctggcga tcctcataaa ggaaatttta tagtatccga
      481 aaaaggttta agactaattg acctttcagg taaaaaaaca accgctgttt taaaagcaaa
      541 agatcgcatt gatcttgaac ggcactataa tataaaaaat gaattga
//
LOCUS       DAZFKM010000554          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFKM010000554 DAZFKM010000000
VERSION     DAZFKM010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..497
                     /locus_tag="ACHKAY_005885"
     CDS             75..497
                     /locus_tag="ACHKAY_005885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO5704282.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          174 a          116 c          158 g          137 t
ORIGIN      
        1 accgggatat tgttcgacag attactgatt cttcccgccg tgttgtatcg gaaaagtgat
       61 tgccggaggc gcttatggca aaagtattta cacaggaaga gcgggaaaaa attaaagggc
      121 aggttgttga gctagtacgc cggagtgggc gcgagacgtt acggcaactg gaagccaaga
      181 caggtgcgac aagatatctg atgagcgttc ttgccagaga gctggttgcc agtggcgatg
      241 tatacaactc tggttacggg ttattcccat ctgaacaggc tcgtaaggac tggcaaaacg
      301 cccgcaaaaa actatcgagg gcaaaactga agaaaccggt tgtggttgat cctgacctta
      361 tctggtcatt accagacgga gaaatacgcc gctacgacag gcgtctgaac ataatctgta
      421 gcgagtgccg gaagagcgaa gctatgcagc gtgtactggc tttctatcag ggtaattttc
      481 agaaggtgct gttgtgagcc aaattaacaa tcggaacttc gtgaagagaa agcataatcc
      541 aaatctgaat aattaagttc agcactgtaa ataaaattta atcct
//
LOCUS       DAZFKM010000555          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFKM010000555 DAZFKM010000000
VERSION     DAZFKM010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..414
                     /gene="espX1"
                     /locus_tag="ACHKAY_005886"
     CDS             <1..414
                     /gene="espX1"
                     /locus_tag="ACHKAY_005886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO5704283.1"
                     /translation="ESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMP
                     GRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKEL
                     NKFMHDYDKTSSEMRKYLKSINECMFLMKNIAHQN"
BASE COUNT          235 a          101 c           87 g          161 t
ORIGIN      
        1 gaaagcgact tatcaaacac catcttttca aatggagaaa ttaataatct cttcattaaa
       61 aaaagcaaca tatttggagc atcattcact aataccagaa taaaaaattt gctctgtgaa
      121 gatattatgc ctggtcgatg gaccactcaa cttgtcaata agcacttagg ttacagatac
      181 acaggagtat tcaaaaccct ggcaagtatt gatgacaaac cgtcccgttt cgaaattttg
      241 ataccattgg tacaaacttt agttcgcgac aatgtaaagc taaataatga tgtgtataaa
      301 gaacttaata agtttatgca cgattacgac aaaacatcat cagaaatgag aaaatacctt
      361 aaaagcatca acgagtgtat gttccttatg aaaaacatag ctcatcaaaa ttaaaaaatc
      421 tcaaaaaaga agtaatttca gaatgaaatt aactgtgatg attattaatt cactacagag
      481 gaataaaaaa caatctgaag catgctctga aacatcctac tctgtcactc taatgatggc
      541 accttgctct tcttataaag aagggaacaa ttaagaatat taaa
//
LOCUS       DAZFKM010000556          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFKM010000556 DAZFKM010000000
VERSION     DAZFKM010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..314)
                     /locus_tag="ACHKAY_005887"
     CDS             complement(<1..314)
                     /locus_tag="ACHKAY_005887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704284.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSS"
     gene            complement(362..571)
                     /locus_tag="ACHKAY_005888"
     CDS             complement(362..571)
                     /locus_tag="ACHKAY_005888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5704285.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
BASE COUNT          117 a          200 c          141 g          126 t
ORIGIN      
        1 gatgactgcg acgcctgttt cagtcgggca ttcacggaac tccattcatc ggccagagaa
       61 atcaggtgtc cggtggcaaa ggcacctgca aatgcccccg ccgttccggc agctgaagcg
      121 cggatttccg tcaactggct gttcagctca gccagggcgc gtcgctgctc ccgggcgact
      181 gcggcagcct gacgcccgcc attctgcagg gtccggtaat attcactgcc catgcgggaa
      241 gcccgctgga tctccgactg gaatgactgc gaatttgccg aaattttgat aatcagttca
      301 cgtaacgtcg ccattcacct ttctccgggc gtaaaaaaac cgcctcagcg gttctcatca
      361 ttcatgactg tgctgcgaag ctcagcgcgt cttccagcgc cgcaaacgga tccacctccg
      421 gcttatcctc atcctcgccc cagcagagca tggcgtcctt cagtgcaaca ttcatcccct
      481 gtgccccaaa aaccgctttc acgatctgtg cattacggat atccccgcgc tcatcaccca
      541 gcggggatac cctgtcgaac tccatccaca tcatcgcctc gctc
//
LOCUS       DAZFKM010000557          577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFKM010000557 DAZFKM010000000
VERSION     DAZFKM010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..186
                     /locus_tag="ACHKAY_005889"
     CDS             <1..186
                     /locus_tag="ACHKAY_005889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO5704286.1"
                     /translation="TSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGEC
                     LPLKKVSLYLNNETPAG"
     gene            282..>577
                     /locus_tag="ACHKAY_005890"
     CDS             282..>577
                     /locus_tag="ACHKAY_005890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5704287.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
BASE COUNT          165 a          132 c          134 g          146 t
ORIGIN      
        1 acttcagcat ttgcactgat gatgacggtt tttcttataa cgggtgaatc acagaatgtg
       61 attaccggaa tttatgcaag taaagaatcc tgcctccagg caagagacga gcaaaaaatt
      121 tctggtgaat gcctcccgct aaaaaaagta tcgctgtacc tgaataacga aacaccggct
      181 ggataatcct ccagccatat taacaccata ccaacggatt aaaaatgcca gcaatggcag
      241 ggattcgttc accctgaaat ctgtaatgag gttaaaacaa aatgagtaag gtctttattt
      301 gcgctgctat tcctgatgaa caggccataa aagaagatag cgctgttgcg gtggccactg
      361 ccattgaagc tggtgatgag cgtcgcgcac gcgcaaaatt tcattggcaa tttctggagc
      421 aattccctgc agctcaggac tgcgcttata aatttattgt ctgtgaggat aaacccggca
      481 taccccgccc tgccctcgat tcctgggatg ctgaatatat gcaggaaaac cgctgggatg
      541 aggcgtctgc ttcctttgtc ccggttgaga ctgaatc
//
LOCUS       DAZFKM010000558          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFKM010000558 DAZFKM010000000
VERSION     DAZFKM010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..551
                     /gene="rrrD"
                     /locus_tag="ACHKAY_005891"
     CDS             54..551
                     /gene="rrrD"
                     /locus_tag="ACHKAY_005891"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO5704288.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT          158 a          129 c          155 g          129 t
ORIGIN      
        1 aatctttatt tcaagattaa agaagataag cgtaaggctg cgagaggtga ataatgtcgc
       61 cgtcattacg caaggctgtt gcagctgcta ttggtggtgg ggctgttgcc atagcgtctg
      121 tgctcatcac tggtccaggt ggtaacgatg gtctggaagg tgtcagctac ataccataca
      181 aagatatcgt tggcgtatgg actgtatgtc acggacacac cggaaaagac atcatgcccg
      241 gtaaaacgta taccgaagca gaatgcaaag ccctcctgaa taaagacctt gccacggtcg
      301 ccagacaaat taacccgtac atcaacgtcg atataccgga aacaacgcgc ggcgctcttt
      361 actcgttcgt ttacaacgtg ggcgctggca atttcagaac atcgacgctt cttcgcaaaa
      421 taaaccaggg cgatatcaaa ggcgcatgtg atcagctacg gcgctggaca tacgctggcg
      481 gtaagcaatg gaaagggctg atgactcgcc gcgagattga gcgtgaagtc tgtttgtggg
      541 ggcaacaatg agcagggtaa ccgttattat c
//
LOCUS       DAZFKM010000559          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFKM010000559 DAZFKM010000000
VERSION     DAZFKM010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..195
                     /locus_tag="ACHKAY_005892"
     CDS             <1..195
                     /locus_tag="ACHKAY_005892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704289.1"
                     /translation="GYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLSTSFNAGSKRF
                     VIYQSAQDKNLQVDIVRTRI"
     gene            281..439
                     /locus_tag="ACHKAY_005893"
     CDS             281..439
                     /locus_tag="ACHKAY_005893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO5704290.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
BASE COUNT          157 a           78 c          133 g          200 t
ORIGIN      
        1 ggatattaca aaccacagtt taagaaagaa tatgaaatga ttaattataa tgatggttgg
       61 tattatgttc ttgctcgtta tgataattgt ctggttttgt ctacttcttt caatgcaggt
      121 agtaaaaggt ttgtcattta tcaatcagca caagataaga atcttcaggt tgatattgta
      181 aggaccagaa tttaattggc tgcataaata atattttaag ttgcaagttg gctattcgta
      241 ggaatagaac cttaggcatg ctgaatgcgt tttctgaaca ttgttttata aactgtgtct
      301 gcttgctgtt gtgatcctgc ttttagtgat ggtgatgatg gatttcacca gcaggataat
      361 gttggtactg actgatggcg ctctggtctg cggcattgtg gtattgctgt ggccgatgat
      421 gaaagaacag aatgaataat tcttgacttt tttgtttact gtttattaaa aaatcaaccg
      481 catggtgaat cctccttgga ggggctaaat gatcgagttt taagggcacg tagcgagttc
      541 tgtttgatca ttgcagaact tagcggga
//
LOCUS       DAZFKM010000560          561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFKM010000560 DAZFKM010000000
VERSION     DAZFKM010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          170 a          114 c          107 g          170 t
ORIGIN      
        1 gtttccctac cttatatacg tttagctcgc ctacggcttg atttaagcgg tagaaactta
       61 tttaaccttc aactactaaa accattcttt cttttttgtt tgttgttggt tgttgtctgt
      121 gtctatgtgc taacatcgct aacgcgtagc acacaaggtt attgcactcg tcagagtatt
      181 gcacaataga ttttctctta aatagattga tgatcgttag attggcgtaa gcctcatcaa
      241 tggaactatt gaagcgcaca gcgcatagac actaacaacc attcgcttac gctcaggtta
      301 ttgcacaagt aaatttcaat cgttagatgg tagttagtta attatcgttc tgcgcacagc
      361 gcaagactat tagccatacg caagccgtag gctatttgca catagtagat tacatattcg
      421 ttgtgagcgg aaagcgaaca tagatcagct aactactaac aaaaaacaaa aaacaaaaac
      481 tatctgttat gacaagcatc agtctaactt gtgcaaggtt ctggcgaatg ccaatagatt
      541 aagggatagt cgaagactcc c
//
LOCUS       DAZFKM010000561          561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFKM010000561 DAZFKM010000000
VERSION     DAZFKM010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>561)
                     /locus_tag="ACHKAY_005894"
     CDS             complement(<1..>561)
                     /locus_tag="ACHKAY_005894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704291.1"
                     /translation="LNSNLKNEVVLSGVMTGTSKVFHLNKSGDLLLTTSKTHGGGVIV
                     IFKDFINNWWKYNLTLITVPIDDKLSDNRINITPMGITIHEIVNGDDKLFFYPTPLKN
                     GGFILHNPLYTNSFPSSYSHELFDLVEAYRNPTYSYLQNSIINRYIHTVSEHNGKDGV
                     AQMSLPIYAYSTCRFWPKESVAVGDT"
BASE COUNT          188 a           96 c           81 g          196 t
ORIGIN      
        1 ttgtatctcc aacagctaca ctttcttttg gccaaaaacg acatgttgag tatgcatata
       61 taggtaatga catctgagct acaccgtctt ttccattatg ctcagaaaca gtatgtatat
      121 atctattgat aatactattc tgtaaatatg aatacgttgg atttctatat gcctcaacca
      181 aatcaaataa ttcatgtgaa tatgaagaag gaaatgaatt agtgtaaagt ggattatgta
      241 agataaaacc tccatttttt aatggagtag gataaaaaaa caacttatca tccccattga
      301 ctatttcatg tatcgttata cccattggtg ttatatttat cctgttatct gacaatttgt
      361 catcaatagg aactgttatc aatgtaaggt tatatttcca ccagttattt ataaaatctt
      421 taaaaataac tataaccccg cccccatgag tttttgaagt ggttaacaat aagtcgcctg
      481 atttatttag atgaaaaacc tttgatgttc ctgtcattac tccagacaaa acaacctcat
      541 ttttgaggtt gctatttaaa c
//
LOCUS       DAZFKM010000562          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFKM010000562 DAZFKM010000000
VERSION     DAZFKM010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>560)
                     /locus_tag="ACHKAY_005895"
     CDS             complement(<1..>560)
                     /locus_tag="ACHKAY_005895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO5704292.1"
                     /translation="TESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPEAYF
                     RMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGEIHKFRC
                     VPHLTGRRFEHGVTDCYTLFRDAYHLAEIEMPDFVREDDWWRNGQNLYLDNLEATGLY
                     QVPLSAAQPGDVLLCCFGSSVPNHAA"
BASE COUNT          128 a          200 c          138 g           94 t
ORIGIN      
        1 atagcggcgt gattcggcac cgatgaacca aaacagcaca gcagcacatc gcccggctgc
       61 gccgctgaca acggcacctg atacagccct gtggcctcca gattatccag atagagattc
      121 tgaccgttac gccaccagtc atcctcacgc acaaaatccg gcatctcaat ctccgccaga
      181 tgataagcat cccggaacag tgtgtaacag tccgtcacac cgtgctcaaa gcgccgcccg
      241 gtgagatgcg gcacacagcg gaacttatga atctcacccc ggcagaccag ccaccacggc
      301 aaatcactct gcacctgcag ccgccggtcg gcctcactca gccagggcag accaccgggg
      361 tggctgtgga ccagcgccac aatctccccc tgcatttctg cctgcagcca gtcttccggc
      421 gacatacgga aatacgcctc cggctcaccg gagatattca cgcaggggaa atatctttcc
      481 ccctccggcg tgcttaccac gaagccgcac gactccgctg gcgcacatcg ccgggcgtgc
      541 gccagaatcg ctgattctgt
//
LOCUS       DAZFKM010000563          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFKM010000563 DAZFKM010000000
VERSION     DAZFKM010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>560
                     /locus_tag="ACHKAY_005896"
     CDS             <1..>560
                     /locus_tag="ACHKAY_005896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO5704293.1"
                     /translation="ATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKRGQ
                     NDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTE
                     SEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKF
                     SNNWQKQKRKIQRLHSCIANIRRDYL"
BASE COUNT          158 a          146 c          139 g          117 t
ORIGIN      
        1 gccactgaaa cgcaatggct taaagattct ccctcacagc cattgcaaca gtcactgaaa
       61 gaccttgagc gggcttacaa aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa
      121 aagcggggac agaatgatgc attccgctac ccgcagggcg ttaagctcga tcaggaaaac
      181 agccgtattt ttctgccgaa actgggctgg atgcgctacc ggaatagccg tcaggtcacg
      241 ggtgttgtga aaaatgtcac tgtcagccag tcctgcggta agtggtacat cagtattcag
      301 acagaaagtg aagtatccac tccggctcac ccttcagcat caatggtcgg gctggatgct
      361 ggcgtggcta aactcgccac gctgtcagat ggcacagtct ttgagcctgt aaacagtttt
      421 cagaaaaacc agaagacgct ggcgagactt cagcgacagt taagccgcaa ggtcaaattc
      481 agcaacaact ggcagaagca gaaacgcaaa atacagcgac tgcattcctg tatcgcaaat
      541 atccgcaggg actaccttca
//
LOCUS       DAZFKM010000564          558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFKM010000564 DAZFKM010000000
VERSION     DAZFKM010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>558
                     /locus_tag="ACHKAY_005897"
     CDS             <1..>558
                     /locus_tag="ACHKAY_005897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO5704294.1"
                     /translation="RMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGK
                     RFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQ
                     DLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEY
                     KKLFALPDESELQQKDAEELFDWLI"
BASE COUNT          159 a          110 c          135 g          154 t
ORIGIN      
        1 cagaatgacg ttatccctca tggtgcaacc cggtcttttt gaccgatata tggagcgaaa
       61 aggttctgtt gccagggaca gtggttttct tgcccgttgt cttatcagta aacctgcaac
      121 cacgcagggg aaacgcttta ttaacggagc tgtgattccg ggaggatcat taacggcatt
      181 tcatgaacga ttaatggaac tggccagagg cagtattgaa aaatccagtg aggatgaacg
      241 gtattgtctg catttttcgc ctgaagcgca aaaaatattt attgagcact ataatgttct
      301 ggagcaggat ttatcccctt ccggaccctt aagcccgttt cggggacatg tttcaaaaaa
      361 aacagaaaac attgcaagaa ttgcagcgtt atttcagtat ttcagctatg gtgaagggaa
      421 aatctctgcc gatatcatga cgtctgctgt cgtaataagt tcgtggtaca cagatgaata
      481 taaaaaatta tttgcgttac ctgatgaatc tgaactgcag cagaaggacg ccgaggagct
      541 gttcgactgg ctcattga
//
LOCUS       DAZFKM010000565          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFKM010000565 DAZFKM010000000
VERSION     DAZFKM010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..>556
                     /locus_tag="ACHKAY_005898"
     CDS             51..>556
                     /locus_tag="ACHKAY_005898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704295.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIV"
BASE COUNT          121 a           81 c          135 g          219 t
ORIGIN      
        1 ctgtaacctc ttgacggcat tgaattgctt ttgttatgag ttgtaagcca atgttatcat
       61 cttgtattgg ggtggttatg aaggatggtg cgctgctcag gagttcttca ctttttattg
      121 cctacatggg atgccttgga tgggggagtg cttatttcta tggatggggt acttcttttt
      181 actacggctt cccatggtgg attgtaggtg caggtgttga tgatgttgcc agaagtttat
      241 tttttgcagt tatcgtcatt gctatatttc ttatcggttg gggtattggt gttgtattct
      301 ttttcgcagt gaaaagaaaa cattctatgc aagagctaaa tgtatttcgc ctttattttg
      361 ctgtggaatt attgtttgtg ccggcaatta ttgagttttc tatattgaga cagaagattc
      421 aggtacctct tttgctactg tcagcagcga ttgcgctggc ggttacaatt tcgataagat
      481 cttatgggcg atttttatcg gtatcatgct tctatgataa gccatttata aaaaaacatt
      541 tttttgagat tgtgat
//
LOCUS       DAZFKM010000566          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFKM010000566 DAZFKM010000000
VERSION     DAZFKM010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>555
                     /locus_tag="ACHKAY_005899"
     CDS             <1..>555
                     /locus_tag="ACHKAY_005899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO5704296.1"
                     /translation="SGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEM
                     KTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYV
                     AGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFMNDVFLKRLFA
                     ASITSSGNPPTFSLTPEGKLTARNA"
BASE COUNT          155 a          137 c          163 g          100 t
ORIGIN      
        1 cagcgggcag cccagcaatg atggtgaagg gtatctggaa tttttccggg aaaaaatagg
       61 aaaactgcat ctggctcagg ggctatggga gctgatagac aacagccagc ttgcggatga
      121 gatggcggag atgaagacca ccatcaccga aacccgcaat gaaatcacac agacggtcag
      181 taaaacgctg gaagaccaga gcgccaccat tcagcagata cagcgcgtgc agaaggacac
      241 aaatgatgac ctggctgcgc tgtacatgct gaaggttcaa aaaacgaaag acggcattcc
      301 ctatgtggcc ggtattggag cggggattga ggatactgat ggccagccac tgagcaacat
      361 actgctgctg gctgaccgta tcgcgatgat aaatccggag aacggcaaca gcactccgtt
      421 atttgtggcg caggggaatc agctgttcat gaacgacgtg ttcctgaagc gtctgtttgc
      481 ggcgagtatc acgtcatccg gcaacccccc gacgttttcc ctgacgccgg aagggaagct
      541 gacagccagg aacgc
//
LOCUS       DAZFKM010000567          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFKM010000567 DAZFKM010000000
VERSION     DAZFKM010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>555)
                     /locus_tag="ACHKAY_005900"
     CDS             complement(<1..>555)
                     /locus_tag="ACHKAY_005900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704297.1"
                     /translation="LVADDGDVFYRITPDRNCGITESVTVPAREVIHDRFNCFFHPLV
                     GLPPVYAAGLAAMQGHHIQANSTYFFRNGGRPSGVIEVPGSITEENAKKLKGNWDSGY
                     TGENAGKTAILSNGAKYSPTTFSPVDAQTVEQLKMTAEIVCSVFRVPAYKIGVGHPPS
                     SDNVEALEQQYYSQCLQTLIESIE"
BASE COUNT          115 a          175 c          139 g          126 t
ORIGIN      
        1 gctcaatgga ctcaatcagc gtctgcagac actgggaata atactgctgc tccagcgcct
       61 ccacgttgtc actggaaggc gggtggccaa cgccaatctt gtaggccggg acacggaaca
      121 ccgaacagac aatttcagcc gtcattttca gttgttccac cgtctgcgca tccaccggtg
      181 aaaacgtcgt ggggctgtat tttgccccgt tgctcagtat ggccgtcttc ccggcatttt
      241 cgcccgtata tccgctgtcc cagttcccct tcagtttttt cgcgttttct tccgtaatac
      301 tgccggggac ctcaatcacg cctgatggtc gcccgccatt tctgaaaaaa tacgtcgaat
      361 ttgcctgaat atgatgcccc tgcatggcgg ccagtcctgc ggcatacacc ggcggcagcc
      421 ccacaagcgg atgaaaaaaa cagttaaaac ggtcgtggat cacctcccgg gcaggcaccg
      481 tcaccgactc tgtgatccca cagttccggt ccggcgtgat gcggtagaac acgtcgccgt
      541 catccgccac cagag
//
LOCUS       DAZFKM010000568          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFKM010000568 DAZFKM010000000
VERSION     DAZFKM010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..550
                     /locus_tag="ACHKAY_005901"
                     /pseudo
     CDS             1..550
                     /locus_tag="ACHKAY_005901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          180 a           85 c          141 g          144 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaacaa cagattgctc
      481 agatagatgt agtgattata ttaatccaga gcataaaaaa acgataaagg ctttacaaga
      541 tgctggctat
//
LOCUS       DAZFKM010000569          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFKM010000569 DAZFKM010000000
VERSION     DAZFKM010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            163..534
                     /gene="ler"
                     /locus_tag="ACHKAY_005902"
     CDS             163..534
                     /gene="ler"
                     /locus_tag="ACHKAY_005902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO5704298.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
BASE COUNT          192 a           85 c          114 g          152 t
ORIGIN      
        1 aacttttata aggattttgt ttgatgaagt agatgtgttc taatttgata gataaaacgt
       61 tatctcacat aatttatatc attcgattaa ttgttgatcc ttcctgataa ggataagatt
      121 gctaatagct taatatatta aagcatgcgg agattattta ttatgaatat ggaaactaat
      181 tcgcacacaa caagcccata cattcagctt attgagcaaa ttgaagtgtt acaacaggaa
      241 gcaaagcgac tgcgagagca ggaaattcaa agtgtaattg agtcgattca aaagcagatt
      301 acttattaca atataaccct acaagagctg ggatatacta atgtgcctga tgatggcctt
      361 gctcgccgga actcatcgaa aggagtttat tatcgcaatg aagaagggca gacctggtcg
      421 ggagttggcc gacagccacg ctggcttaaa gaagcactgt tgaatggaat gaagaaagaa
      481 gattttcttg tgaaggacac cgaagacgaa ataataccgc tgaaaaatat ttaacatgaa
      541 atc
//
LOCUS       DAZFKM010000570          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFKM010000570 DAZFKM010000000
VERSION     DAZFKM010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..>538
                     /gene="espL"
                     /locus_tag="ACHKAY_005903"
     CDS             3..>538
                     /gene="espL"
                     /locus_tag="ACHKAY_005903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFO5704299.1"
                     /translation="MIQSDDIVKLARKKTCEHLLRARRKSKKLKTESRKKIARKIMAM
                     RERIRKNNKIKLEKEVNKSIKWVKDIQDIELVLMQDIMNKVHSSLTNALHSLDTSSRI
                     NWDDLLNEVVRETLSHNNIVGAIKITKNPDIKLDPGEANNIQLINDADAPLNKIIIEN
                     EYMRITLDPLEQINILLNS"
BASE COUNT          231 a           69 c           96 g          142 t
ORIGIN      
        1 aaattattca atctgatgat atagtcaagt tggctcgaaa aaaaacatgt gaacatttac
       61 tgagggcgag gaggaagtcg aaaaaattaa agacagaatc cagaaaaaaa atcgccagaa
      121 aaataatggc aatgagagaa aggattcgaa aaaataataa aataaaatta gagaaagaag
      181 ttaacaaatc gataaaatgg gttaaagata ttcaggatat cgagttggtc ttaatgcagg
      241 acataatgaa taaggttcat tcgtcactga ctaatgcatt acattcttta gacacatcat
      301 caagaattaa ttgggatgat ttacttaatg aagtcgtaag ggaaacgctt tcccataaca
      361 atattgttgg agctattaag atcactaaaa acccagacat taaacttgat cctggagagg
      421 ctaataatat acagcttatt aatgatgcag acgcaccact taataagata ataatagaaa
      481 atgaatatat gagaataact ttagatccgt tagaacaaat aaatatttta ttgaattc
//
LOCUS       DAZFKM010000571          536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFKM010000571 DAZFKM010000000
VERSION     DAZFKM010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(122..331)
                     /locus_tag="ACHKAY_005904"
     CDS             complement(122..331)
                     /locus_tag="ACHKAY_005904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO5704300.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(427..>536)
                     /locus_tag="ACHKAY_005905"
     CDS             complement(427..>536)
                     /locus_tag="ACHKAY_005905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5704301.1"
                     /translation="PDDREQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          119 a          177 c          124 g          116 t
ORIGIN      
        1 cgctggagct ccgactggaa tgactgtgaa tttgccgaaa ttttgataat cagttcacgt
       61 aacgtcgcca ttcacctttc tccgggcaaa aaaacctgcc acagcaggtt ttcatcatta
      121 tttatgacat tgctgcaaag ctcagcgcgt cttccagcgc cgcaaacgga tctgactcca
      181 ctttatcctc atcctcgccc cagcagagca tggcgtcctc cagtgaaaca ttcatcccct
      241 gcgccccgaa aaccgctttc acgatctgcg cattacggat atccccgcgc tcatcgccca
      301 gcggggacac cctgtcgaac tccatccaca tcatcgcctc gctcgcactc agactgtggc
      361 ccagttcgga taaggtgcgc cccagacgga gcgcaagtcg catcagaaag agaatttccg
      421 ggcgggctac ttttttctgg ccgactctgc atcagcgatc agttccagtg cctgacgcag
      481 caaccgggca tgtaccggac catagacggc cagcacctgc tcacggtcgt ccggag
//
LOCUS       DAZFKM010000572          530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFKM010000572 DAZFKM010000000
VERSION     DAZFKM010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..428
                     /locus_tag="ACHKAY_005906"
     CDS             <1..428
                     /locus_tag="ACHKAY_005906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704302.1"
                     /translation="DLGGKSTYRIIKGEKAMKLLPLGTKFYTAPPAPIALEAIENAIE
                     YIRSIAFHIDEDDYHGKHIAYFMRQALAWLEGHSCSDDSQGKSDNSPLPRYQVIELTM
                     LVKQLVSQLKKAKPDCKLPDRAMDYLLRNGLVSAEDVLR"
BASE COUNT          162 a          106 c          129 g          133 t
ORIGIN      
        1 tagaccttgg cgggaaatca acttatcgca tcattaaggg agaaaaagcg atgaagttgt
       61 tgccgctggg gacgaagttt tataccgcac cgccagcgcc gatagcgttg gaggccattg
      121 aaaatgcaat agaatacatc cgcagtatcg cttttcacat cgatgaagac gattaccacg
      181 gcaaacatat tgcgtatttc atgcgacaag cattggcctg gctggaaggg cattcatgca
      241 gtgatgacag tcagggcaaa tccgataatt caccattgcc gcgctaccag gtgatcgaat
      301 taacaatgct ggttaaacaa ttggtcagcc aactgaaaaa agcaaaacct gattgcaaat
      361 tacctgatag ggcgatggat tatcttttgc gaaacggact ggtaagtgcg gaggatgttt
      421 tacgatgacc atttctacaa aaaagcctct ttatgctgag gttcacaatg ttcctgatga
      481 ttatgagttc actgacgagg aactaaacag aattattgca ggtgatatgt
//
LOCUS       DAZFKM010000573          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFKM010000573 DAZFKM010000000
VERSION     DAZFKM010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..283)
                     /locus_tag="ACHKAY_005907"
     CDS             complement(<1..283)
                     /locus_tag="ACHKAY_005907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704303.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNH"
BASE COUNT          165 a           97 c          105 g          162 t
ORIGIN      
        1 catgattcag gtaatatgta gagatagtcc cgttaacagt gtcaccatta aaggtggtat
       61 cgaaatcacc gtttgtgttg ttatagtaga caaagccact ataattcaca tagttaccat
      121 ctacagtacc cggtgcatcc atattgctcc agtcaagagc ggtagttgga ttgtcgtaat
      181 aagtccaggt gtcataagta gttgcattag cctgagaggt gatagccaga gtgcaagcca
      241 atgctaattg tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag
      301 ggacgtaata agttgatcca tcaattatca atgcatagaa aacatattat gtttcgcgct
      361 aatgattatg ctgcgttaca gacgaatggt tcaattattc tttcccctaa gtccgaatgt
      421 ggacatatat aacctatgga gaaaaacacg catcaaattg ataaaagggc gatatttcaa
      481 catgttgcag ataatgttat ttttgataca tacgcgttat aaatatcaa
//
LOCUS       DAZFKM010000574          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFKM010000574 DAZFKM010000000
VERSION     DAZFKM010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>528
                     /locus_tag="ACHKAY_005908"
     CDS             <1..>528
                     /locus_tag="ACHKAY_005908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5704304.1"
                     /translation="AEYMQENRWDEESASFVPVETESDPINVTFDKLAPEVQNAVMVK
                     FDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHK
                     CKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARANQQHTENSTGKISPVI
                     AAIHREYKQTWKTLD"
BASE COUNT          164 a          118 c          136 g          110 t
ORIGIN      
        1 tgctgaatat atgcaggaaa accgctggga tgaggagtct gcttcctttg tcccggttga
       61 gactgaatca gatccgataa acgtcacttt tgacaagctg gcccctgaag tacagaacgc
      121 tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt gatatggtga ttagcgcgca
      181 ggaactgttg caggaagaca tggcaacatt cgacggacat atcgttgaag cgttgatgaa
      241 aatgccagaa gttaacgcca tgtatccgga gcttaagctg catgccatcg ggtgggttaa
      301 gcataaatgt aagccaggtg ccaaatggcc cgaaattcag gcagagatgc gcatctggaa
      361 aaaacgtcgc gaaggtgaac gcaaggaaac cggaaaatac acgtctgttg ttgatctcgc
      421 ccgcgccaga gccaatcaac agcacactga aaattcaaca ggaaaaatca gcccggtcat
      481 tgctgccatt catcgcgaat acaagcagac atggaaaaca ctggatga
//
LOCUS       DAZFKM010000575          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZFKM010000575 DAZFKM010000000
VERSION     DAZFKM010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..255)
                     /locus_tag="ACHKAY_005909"
     CDS             complement(34..255)
                     /locus_tag="ACHKAY_005909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO5704305.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(318..>527)
                     /locus_tag="ACHKAY_005910"
     CDS             complement(318..>527)
                     /locus_tag="ACHKAY_005910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO5704306.1"
                     /translation="EVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGL
                     VDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT          128 a          136 c          153 g          110 t
ORIGIN      
        1 gccagtcgta ctcgttttcc gggtgacgga acatcagttc agggtgacgc tcatcagccg
       61 gacatacgga ccaaaactgt ccttacggcg ttctgcaaac acggccagca caccgggaat
      121 atcctgcact tcacgaccgg tatacgcttc agcactgccg tgccagcggt atttgccggt
      181 acagaacgga aaaagacggg atgccggatg ctgacggtga atacgcatgg cttcgccacg
      241 ggtgatgatt ttcatagtgg gatacctctg aagacagaag ataaaagtga aaacaggtgt
      301 gatgtggttg tgacgggtta aagcagacca tgttcggcaa aggagaaaac ctggctgcca
      361 ccgactatca gatggtccgg cacccggata tccaccaggc ccagtgcctg taccagacgt
      421 tccgtgataa ggcggtctgc cttgctgggt gtgacttcac cggacgggtg attgtgtgcc
      481 agcaccacag cagcggcatt gtgatacagg gcgcgtttaa tcacttc
//
LOCUS       DAZFKM010000576          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFKM010000576 DAZFKM010000000
VERSION     DAZFKM010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>526
                     /locus_tag="ACHKAY_005911"
     CDS             <1..>526
                     /locus_tag="ACHKAY_005911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO5704307.1"
                     /translation="WMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLS
                     GLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHS
                     AIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEALKHIEKNINDNIIYHP
                     SNNANFYSVIESIDG"
BASE COUNT          174 a           66 c          117 g          169 t
ORIGIN      
        1 tggatgtcaa aatcagaaaa gaataataaa caaaaagagg caatagaaaa gttcaaaaaa
       61 gaagtatccc atatgaatgg cgggcagcag tttatttggg ggtttgataa ggttattcaa
      121 gaaggcttaa gtggattgat tgagttaagt atcgatatta atgatagtac aaatcatcgt
      181 gataagtctt ctctttctcc tgatgggaga gctgtgttac attttttagg tacaatttgg
      241 aatatggcga tgggagctgt acctggttat aatgcattgt ctggtgtaag tagcatttta
      301 catagtgcta tagtaaaaga atctagcaat atctgtgatt atattcaggg ggctgtacgt
      361 attggaatgg actttgttcc aggcactcgc tctgacttac atagccgttc gctgcagata
      421 aaatatgagg ctttgaagca tatagaaaaa aacattaatg ataatattat ttatcatccg
      481 agtaataatg ctaatttcta ttctgtaatt gagtcaattg atggta
//
LOCUS       DAZFKM010000577          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFKM010000577 DAZFKM010000000
VERSION     DAZFKM010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..91
                     /locus_tag="ACHKAY_005912"
     CDS             <1..91
                     /locus_tag="ACHKAY_005912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5704308.1"
                     /translation="HLQVRFGDGPWQDVKGLNEVGSDTGRTGE"
     gene            93..362
                     /locus_tag="ACHKAY_005913"
     CDS             93..362
                     /locus_tag="ACHKAY_005913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5704309.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          146 a           94 c          155 g          126 t
ORIGIN      
        1 gcatttgcag gtcaggtttg gtgacgggcc gtggcaggat gttaaggggc tgaatgaagt
       61 gggcagtgat acaggcagaa caggagaatg acatgaatat actaaaaaaa cttatgcagc
      121 gtctgtgtgg ttgcggaaag catgatggcc gtgaacacgt gcagtcgctt acagcacaac
      181 tgcgactggg accggcagac attctggagt cagatgagaa tggcattatt ccggagcagg
      241 acagggtaat cacacaggtg gtgatactgg atgcggataa aaagcagata cagtgcgtgg
      301 taagaccgct gcaaattctg cgtgctgacg ggacgtggga aaatattggc gggatgaagt
      361 aacccgacag cttcacaaaa ccggagtccg gctccggttt ttgtgttgcc atataaggca
      421 gatgtttgtt acagctattt aagtctggag ttcaaataaa aatagggagt tttgttatgc
      481 cattaatccc ggatattaat tcatcttcgt tcagtcctga g
//
LOCUS       DAZFKM010000578          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFKM010000578 DAZFKM010000000
VERSION     DAZFKM010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..93)
                     /locus_tag="ACHKAY_005914"
     CDS             complement(<1..93)
                     /locus_tag="ACHKAY_005914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704310.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGE"
     gene            complement(104..>511)
                     /locus_tag="ACHKAY_005915"
     CDS             complement(104..>511)
                     /locus_tag="ACHKAY_005915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5704311.1"
                     /translation="LVGATVVRRRVYARFLDAVNFVAGNPEADPEQELRDRWVVEQMS
                     ELTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPTTD
                     IRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
BASE COUNT          107 a          185 c          123 g           96 t
ORIGIN      
        1 ctccccctcc ggcgttctca ccacgaagcc gcacgactcc gcaggcgcac accgccgggc
       61 atgcgccaga atcgctgatt cagtctgtgt cataaaccgg gatttactgc gaaagtttat
      121 taatggaaag gaaaccgccg aaattagcca ccatgccgcg catctcacac ccgcgcatgc
      181 atttactgca tctgtccttc cggatatccg tggtggggtt gtcgaactca tccgccaccg
      241 caggaccgtg ataaccgcat tcatctcccc ggtaatccca catacaggtg ttcgccagca
      301 tgatgcgacc gggaaacagc gctccgtccg tctccgtcgg tgttgccagc acaaacgagg
      361 ccgtcatggc cgtcagctct gacatctgct ccaccaccca gcggtctctc agctcctgct
      421 ccgggtccgc ttccggattg cccgccacaa aattcaccgc atccagaaaa cgggcataca
      481 cccggcggcg gaccaccgtg gcccccacca g
//
LOCUS       DAZFKM010000579          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFKM010000579 DAZFKM010000000
VERSION     DAZFKM010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>506
                     /locus_tag="ACHKAY_005916"
     CDS             <1..>506
                     /locus_tag="ACHKAY_005916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704312.1"
                     /translation="VPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADGQYL
                     AQAKWDTPKVVKGVSFMLRLTVVADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRA
                     VNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPRLAVYDPTVQFEFWFSETR
                     ITDIRQVET"
BASE COUNT          106 a          136 c          174 g           90 t
ORIGIN      
        1 gtgccggaaa aagaagccat cgtggataac ggggccagct ttgagccgca gtcaggcacc
       61 ctgaacagcg tcatccctcc ggcagtgcag cacctgacgg tggaggtgag cgcagctgac
      121 ggtcagtatc tggcgcaggc gaaatgggac acgccgaagg tggtgaaggg cgtgagcttt
      181 atgcttcgcc tgaccgtggt cgcggatgac ggcagtgagc ggctggtcag cacggcccgg
      241 acgacggaaa ccacataccg cttcacgcaa ctggcgccgg ggaactacag gctgacagtc
      301 cgggcggtaa atgcgtgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcc
      361 gcaccggcag cgccgtcaca gattgagctg acaccgggct attttcagat aacagcggtc
      421 ccgcgtcttg cggtgtatga cccgacggta cagtttgagt tctggttttc ggaaacgcgg
      481 attaccgata tcaggcaggt tgaaac
//
LOCUS       DAZFKM010000580          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFKM010000580 DAZFKM010000000
VERSION     DAZFKM010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..268
                     /locus_tag="ACHKAY_005917"
     CDS             50..268
                     /locus_tag="ACHKAY_005917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704313.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINVRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT          155 a           63 c          103 g          180 t
ORIGIN      
        1 tgggaactgg acatataatt actcttctgt gctaaaggaa cgttctgaaa tgtttaagta
       61 tgcagagatg ttgatggcgg aagaagatga taaagtatgt gaacttattt ttaaacaaca
      121 agataatttg attttaaaaa taaatgttag atttcccaga ataattataa agaaataccg
      181 aacattacaa ggtaaagtcg tatggctatg gatattgcca tttgttattg aatggttaat
      241 tgtgtgtact ttgggtgtaa acagttagct tggtaaaagt aaatatcctg attgttgttt
      301 gttaaggagt gagatgcctg tgcgtctggt gtctattata ttcccaatat tattggcgct
      361 attcttgctg gcgtaggcat cgccatacta tgaatattgt gtttgtcgtg attcaatttt
      421 tatttataac ctctacccgg agcaaattca ggtaggtcat gcataaaaat atctttctat
      481 actacgaaaa ttatttttgt g
//
LOCUS       DAZFKM010000581          493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZFKM010000581 DAZFKM010000000
VERSION     DAZFKM010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            33..>493
                     /locus_tag="ACHKAY_005918"
     CDS             33..>493
                     /locus_tag="ACHKAY_005918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO5704314.1"
                     /translation="MLCYLIAIHFVIFGNSKALLSYWMAEIIRREKMDVWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAARKLNNNIINRFGCEIGLGANIGKGLTIPHHT
                     GIVVHFAVDAGENLVLRQNTTIGQVDGDMPGSRVKIGNNVDIGANCCIIGLS"
BASE COUNT          165 a           69 c          110 g          149 t
ORIGIN      
        1 catagaaacc aaaaaatata ggcagttgca atatgttatg ttacttaata gctattcact
       61 ttgtcatctt cgggaattca aaagctctat tgagctactg gatggctgaa atcatcaggc
      121 gtgaaaaaat ggatgtttgg cgactattaa gagagaagaa gcagcgcaat cggaatttcc
      181 ttttctggtg gagactggct aatgaaatgt atattaatgg caataaatta cataagaaag
      241 cagccagaaa gttaaacaat aatataatta acagatttgg ttgcgaaatt ggattgggcg
      301 caaatattgg aaaaggttta acaattcccc atcatactgg aattgttgtt cattttgctg
      361 ttgatgctgg tgaaaatctg gttttgcgac agaatactac cattggacag gtagatggtg
      421 acatgcctgg ttcaagagta aaaattggta ataacgttga tatcggagct aattgttgca
      481 tcattggatt atc
//
LOCUS       DAZFKM010000582          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFKM010000582 DAZFKM010000000
VERSION     DAZFKM010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>483)
                     /locus_tag="ACHKAY_005919"
     CDS             complement(<1..>483)
                     /locus_tag="ACHKAY_005919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO5704315.1"
                     /translation="HRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEAD
                     EVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQ
                     RAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLV
                     R"
BASE COUNT           79 a          168 c          156 g           80 t
ORIGIN      
        1 gcgcaccagc ggcgcggcag gtaaattctc cgggtattcc gggtcgtgcg tcagccacac
       61 ggcagacaga cggatgccgt tgtcccggcc atattcggtg taacccggca gggtatcagg
      121 aaaagcggac ggttccgtcc cgccggaaat ggcctgctgc cgggcttctt ctgcccgctg
      181 cgcctgcgtg gtcagtacca gccggaagtg acgcccggca ccatccgtca cgccggtgat
      241 ttcgccgctg aattcaccgg cggcttcgcg gtggaacgtc tgtgtgcgcc cgaagcggtc
      301 caccagcccg gtcagtaccc ggtacggcgg cagcggcgca ggcagcacct catccgcttc
      361 cggcacccgc tcacaccagc cgagcagcca ccacggcccc tgcggactgt ttgtcgccag
      421 ataacgatgc ggacttaagc ggagttcttc cggcagcgcc tgccagagtg cggccagccg
      481 gtg
//
LOCUS       DAZFKM010000583          482 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFKM010000583 DAZFKM010000000
VERSION     DAZFKM010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..211)
                     /locus_tag="ACHKAY_005920"
     CDS             complement(<1..211)
                     /locus_tag="ACHKAY_005920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO5704316.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQR"
BASE COUNT          158 a           98 c          116 g          110 t
ORIGIN      
        1 ggcgctgtaa ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc gtccggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cggtaaaatg aataatgatc
      241 cgtaccgtac aactgcggat aatgtgataa gcgggacgac ttcagacgtt attagggaag
      301 gaacagaaaa tgctatggtt atgtgagaat tttgtttaaa tattacttta cagaaacggg
      361 tgtctgttca gtcaaaaaaa taatcacaag ctcaacctct tgtttcttgg cgttattcag
      421 gacaggtacg catatcacaa cgagtaacaa aaacgcaaag ttaaaccaat attcataata
      481 ta
//
LOCUS       DAZFKM010000584          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFKM010000584 DAZFKM010000000
VERSION     DAZFKM010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>480)
                     /locus_tag="ACHKAY_005921"
     CDS             complement(<1..>480)
                     /locus_tag="ACHKAY_005921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HFO5704317.1"
                     /translation="HLFNIINNLDGVKLGFIISLPVFLISALNFVFTPFSFRYIFKPF
                     FCILFICSSIVTYATMKYGVQFDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPC
                     GLLLLVNIRYPETWIKGIIYRLISMFASLLIIFAIAFFFYKDYASVGRNNSSLNKEI"
BASE COUNT          200 a           77 c           66 g          137 t
ORIGIN      
        1 attatttctt tatttaatga agagttgttt ctgccaactg atgcataatc cttataaaag
       61 aaaaacgcaa tcgcaaatat aatcaagaga gatgcaaaca ttgatattaa acgatatata
      121 atacccttta tccatgtctc aggataacgg atatttacta acaacaatag tccacatgga
      181 agtatacccg taaataaaaa ccacagtaca acagacatat tgaaatatga tgtcatttca
      241 cctgcatttg tttcaaaaat attctgcatc attgttttat caaactgcac accatatttc
      301 atggtggcat atgtaacaat ggaactacag ataaacagaa tacaaaaaaa aggtttaaat
      361 atatacctga atgagaacgg tgtaaaaaca aaatttaatg cagatatcag aaagactgga
      421 agagatataa taaatccaag tttcacacca tcaaggttat taataatatt aaacagatgc
//
LOCUS       DAZFKM010000585          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFKM010000585 DAZFKM010000000
VERSION     DAZFKM010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..176)
                     /locus_tag="ACHKAY_005922"
     CDS             complement(<1..176)
                     /locus_tag="ACHKAY_005922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5704318.1"
                     /translation="MRKLCAVILSAVVWLVAAGTPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYE"
     gene            complement(244..>479)
                     /locus_tag="ACHKAY_005923"
     CDS             complement(244..>479)
                     /locus_tag="ACHKAY_005923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HFO5704319.1"
                     /translation="SSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFS
                     VSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          123 a          127 c          107 g          122 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatgccc ttcaggtcat cgctgcctgg catatcagta
       61 tgggtctgaa gatacccggc gcttagtgtg gactgatgct ctgctgcgct cgctggcgta
      121 ccagcggcaa ccagccagac tactgcggac agaataacag cacataattt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaggcg ccatttctgg cgcccgtata tgggttataa
      241 aattcagctg atactgatgc ctgcggtggc tttcttcatc accacaacca gcaaatcgct
      301 gatacttgct gtgggatacc agtcatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacgg ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagt
//
LOCUS       DAZFKM010000586          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFKM010000586 DAZFKM010000000
VERSION     DAZFKM010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>476
                     /locus_tag="ACHKAY_005924"
     rRNA            <1..>476
                     /locus_tag="ACHKAY_005924"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFKM010000587          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFKM010000587 DAZFKM010000000
VERSION     DAZFKM010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>471)
                     /locus_tag="ACHKAY_005925"
     CDS             complement(<1..>471)
                     /locus_tag="ACHKAY_005925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO5704320.1"
                     /translation="WLGDLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKISGVELS
                     DINKEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQLSFYW
                     QGGQCKPEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLPKSGEIKPTHI"
BASE COUNT          145 a           96 c           66 g          164 t
ORIGIN      
        1 taatatgggt tggttttatt tctccacttt tcggtaaaag atatttgact atagtccata
       61 agggattatc agaaaatagc cattcaagat ttcctgcacc gtattttatc agttcagaac
      121 aaaaccgttc ttcgccatat tctggtttac attgccctcc ctgccaatag aatgataact
      181 gcttcacatt ttgatatcct atctctctga tcagagtttc tcgatataat cgtgaaaaaa
      241 tatagtcagg gcattgaatt tcaaataatg cactacttag agcaagcaat cttattttcc
      301 caattgactg atataaaaaa ccttccttat ttatatcaga taattccact ccagaaattt
      361 tatcttgtat ttcctgacgc aatatcttgt tgagttgatt acgagtagaa ctactttctc
      421 ttaaatgccg aaaactaacc tgattgacac cggttaaatc acctagccat t
//
LOCUS       DAZFKM010000588          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZFKM010000588 DAZFKM010000000
VERSION     DAZFKM010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..264
                     /locus_tag="ACHKAY_005926"
     CDS             <1..264
                     /locus_tag="ACHKAY_005926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704321.1"
                     /translation="FEQQIKNALLVASPVPGIRYQVEFDSADLLRATPGERFATYERG
                     IKSGVMCPNEAREREGLSPRDGGDEFSQAWKQEVKISEGEKPE"
     gene            261..>471
                     /locus_tag="ACHKAY_005927"
     CDS             261..>471
                     /locus_tag="ACHKAY_005927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO5704322.1"
                     /translation="MNIGRLRDRVTIQTLKQTRDITGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDIN"
BASE COUNT          130 a          113 c          147 g           81 t
ORIGIN      
        1 tttgagcagc agataaaaaa cgccctgctg gtggcctcgc ctgtacctgg tatccggtat
       61 caggtggagt ttgacagcgc ggacctgtta cgggccacac ctggcgaacg ctttgccacc
      121 tatgaacgcg gcatcaaatc cggcgttatg tgcccgaacg aagcccgcga acgtgaaggg
      181 ctgtccccgc gtgatggtgg tgatgagttc agccaggcat ggaaacagga agtaaaaatc
      241 agcgagggag aaaaaccgga atgaacatag ggcgactgcg tgacagggta acgattcaga
      301 ccctgaaaca gaccagggat ataaccggcg aaatactcga aacgtgggag gacggtcaca
      361 cactctgggc aagcgtgaac atgatcagca gcaaggaggc catttcatcg ggtgcagagc
      421 tggcgattgg taccgtaagg atctggatac ggtaccggaa ggacatcaac g
//
LOCUS       DAZFKM010000589          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZFKM010000589 DAZFKM010000000
VERSION     DAZFKM010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..208)
                     /locus_tag="ACHKAY_005928"
     CDS             complement(<1..208)
                     /locus_tag="ACHKAY_005928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704323.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFL"
     gene            complement(249..377)
                     /locus_tag="ACHKAY_005929"
     CDS             complement(249..377)
                     /locus_tag="ACHKAY_005929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO5704324.1"
                     /translation="MVLVMKYGAITRTANHTAGEIRLFLHQLKRYGALPGFRHGLA"
     gene            complement(334..>468)
                     /locus_tag="ACHKAY_005930"
     CDS             complement(334..>468)
                     /locus_tag="ACHKAY_005930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO5704325.1"
                     /translation="CRKAQRPPEPPDNYGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS
                     "
BASE COUNT          104 a          123 c           92 g          149 t
ORIGIN      
        1 tcagaaattc tatcaatgca tctatctgtt caggatttac cgccagcatt tcaccggata
       61 gagcacatct aacaaaacca tgctgatcct tttcaatcag tgcgcccgtc tccaggaatg
      121 cgcggtaatc attgatgctc atcgtctcca tgttgtcagc atggtattta tcacgctgtt
      181 ttaatatctc atcaaatttc atcggcattg ttttttcctc tgttgtgtct gtttgtttca
      241 gatagtaact atgccagacc gtgacgaaaa cccggtaatg cgccataccg tttcaactgg
      301 tgcaaaaaaa gccggatttc tccggctgtg tgattagctg tcctggtaat tgcgccatat
      361 ttcatcacca gcaccatcta tccccatttc ggcataagtg cgatcgactg cctttttcag
      421 gtctccgtaa ttatccggtg gctccggtgg cctctgtgcc ttcctgca
//
LOCUS       DAZFKM010000590          461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZFKM010000590 DAZFKM010000000
VERSION     DAZFKM010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..301)
                     /locus_tag="ACHKAY_005931"
     CDS             complement(95..301)
                     /locus_tag="ACHKAY_005931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO5704326.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          126 a          109 c           87 g          139 t
ORIGIN      
        1 taacgatgtc taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc
       61 gctggcggtt ttcaatgtat tcaaaactca gcaatcaata aaaatcacca gttgcctttt
      121 cggcctgatc catccacacc ggtttatcgc tggttttcga ccagacgcga tgcaggtagc
      181 tgtaaaagcg ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg
      241 caaccgcata agtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca
      301 tacttttctc ccccttttgt atctacccgg tgaatggtac cggaaaaatg aatttgttta
      361 tctgatgaaa atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt
      421 gcttattgat ggtttattta cattcatcct gtaattaagt t
//
LOCUS       DAZFKM010000591          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZFKM010000591 DAZFKM010000000
VERSION     DAZFKM010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>458
                     /locus_tag="ACHKAY_005932"
     CDS             <1..>458
                     /locus_tag="ACHKAY_005932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO5704327.1"
                     /translation="DPMNVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDV
                     ATFDGHIVEALMKTPEINAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDA
                     ERKETGKYTSVVDLASARVNQQNTENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          147 a          103 c          113 g           95 t
ORIGIN      
        1 gatccgatga acgtcaattt tgacaagctg tcccttgaag tacagaacgc ggtcctggtt
       61 aagttcggta catgtgaaaa catcaccgtt gatatggcga ttgacgcgca ggaattactg
      121 caggaagacg ttgctacctt tgacgggcat atcgttgaag cactgatgaa aacgcctgaa
      181 attaacgcta tgtatccgga acgcaaactg ttcgctatcg gatgggttaa acacaaatgt
      241 aatccgggtg ccaaatggcc cgaaattcag gctgaattac gtaactggaa aaaacggcag
      301 gacgcagagc gcaaagagac tggaaaatac acgtctgttg ttgatctcgc cagcgccaga
      361 gtcaatcaac agaacactga aaactcagca ggaaaaatca acccagtcac tgccgccatt
      421 tgtcgcgaat acaagcagac atggaaaacg ctggatga
//
LOCUS       DAZFKM010000592          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZFKM010000592 DAZFKM010000000
VERSION     DAZFKM010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            141..>453
                     /gene="grlR"
                     /locus_tag="ACHKAY_005933"
     CDS             141..>453
                     /gene="grlR"
                     /locus_tag="ACHKAY_005933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO5704328.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEKLFVDV
                     YA"
BASE COUNT          153 a           58 c          100 g          142 t
ORIGIN      
        1 acaacaggtt ggaatatgag tatatttaat gatatattac attgcaatct ggagaaaaag
       61 aaaggtctcc gttattcttg atatcgctta tggatagaac aaattgaaag gagtgaggtt
      121 agtatgaaac tgagtgagtt atgattatga aggatggcat ctatagcatt atctttatta
      181 gcaatgaaga ttcctgtggg gaaggtatac tgattaaaaa tggaaatttg atcactggcg
      241 gagatattgc ttctgtgtat cagggggtcc tgtctgaaga ggaggacatc atacttcatg
      301 tccatcgcta taatcatgaa attccctcgg tgctaaacat tgaacaagat tatcaattag
      361 ttatccctaa aaaagtactg agtaatgata gtaatgtcac attacattgc catgtaagag
      421 gaaatgaaaa attgtttgtt gatgtttatg cca
//
LOCUS       DAZFKM010000593          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZFKM010000593 DAZFKM010000000
VERSION     DAZFKM010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHKAY_005934"
     CDS             <1..>453
                     /locus_tag="ACHKAY_005934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704329.1"
                     /translation="ESDVQGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMAAQ
                     AQAFEEKLASLSAVLQKHVTVDEVHPVLQAMVDDAVGTIPVPRDGRDYDPDVLQQAVN
                     DAVANIPVPADGKSITPDDVRPMLEQMVKEAVSHIPAPRDGRDYDPEVL"
BASE COUNT          105 a           96 c          152 g          100 t
ORIGIN      
        1 gaaagcgatg ttcaggggga tactgagaaa atgacggaac gggaactgtc cattattcgt
       61 gcactgggcg aagaattctc cacggtgctg gcggatttac agcgcacatt tgaggggaag
      121 atggccgcgc aggcacaagc gtttgaagag aaactggctt ccctgtcggc ggtattacag
      181 aagcatgtga cggtggatga ggtacatccg gttctgcagg cgatggtgga tgacgctgtg
      241 gggaccattc cggtaccgcg tgatggtcgt gattatgatc cggatgtact gcagcaggcg
      301 gtgaatgatg cggttgcgaa tattccggta ccggcggacg gcaaaagtat cacccccgat
      361 gatgtgcgtc cgatgcttga gcagatggtg aaagaggctg taagccatat ccctgctccg
      421 cgtgacggtc gtgattatga tccggaagta ctg
//
LOCUS       DAZFKM010000594          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZFKM010000594 DAZFKM010000000
VERSION     DAZFKM010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /gene="cesT"
                     /locus_tag="ACHKAY_005935"
     CDS             <1..>453
                     /gene="cesT"
                     /locus_tag="ACHKAY_005935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO5704330.1"
                     /translation="SELLLDRFAEKIGIGSISFNENRLCSFAIDEIYYISLSDANDEY
                     MMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLDDATP
                     EKLENEIEVVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYA"
BASE COUNT          148 a           63 c           93 g          149 t
ORIGIN      
        1 gatctgagct tttattagat agatttgcag aaaaaattgg tattggatct atttcattta
       61 atgaaaatag attgtgttct tttgctattg atgagattta ttatatttcg ttatctgatg
      121 ctaatgacga atatatgatg atctatggcg tctgtgggaa attcccgaca gataatccta
      181 acttcgctct tgagattttg aatgcaaact tatggtttgc agagaatggt ggaccatacc
      241 tgtgttatga atcaggagca caatcgctgt tgttagcgtt acgttttccg ctcgatgatg
      301 ctacacctga aaaacttgag aatgaaatag aagtcgtcgt taagtcaatg gaaaacctgt
      361 atttggtatt acataatcag ggaataacat tagaaaacga acatatgaaa atagaggaaa
      421 ttagttcaag cgataataaa cattattacg ccg
//
LOCUS       DAZFKM010000595          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZFKM010000595 DAZFKM010000000
VERSION     DAZFKM010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHKAY_005936"
     CDS             <1..>453
                     /locus_tag="ACHKAY_005936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5704331.1"
                     /translation="VRYTAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          110 a          101 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt acgtgtggat
      181 gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt gatcaccatg
      361 ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZFKM010000596          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZFKM010000596 DAZFKM010000000
VERSION     DAZFKM010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>453
                     /locus_tag="ACHKAY_005937"
     CDS             <1..>453
                     /locus_tag="ACHKAY_005937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5704332.1"
                     /translation="VRYTAAVPSRVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKI
                     IIMGRPDEEGTLLRVDAAINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVF
                     RVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAKEILYARMKADP"
BASE COUNT          108 a          103 c          142 g          100 t
ORIGIN      
        1 gtgcgttaca cggctgcggt gccttcccgg gtggtttatc tgacggcggg cattgactcg
       61 cagcgaaacc gttttgagat gtatgtctgg ggatgggcac cgggagagga agcctttctg
      121 gtggataaaa tcatcattat ggggcgtccc gatgaggaag ggacgctgtt acgtgtggat
      181 gctgcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgaccat ttcccgtgtc
      241 tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa aaaacacggt
      301 gttttccggg tgctgccggt aaaaggcgcg tctgtctatg gcaagccggt gatcaccatg
      361 ccgaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga caccgcaaaa
      421 gaaattctct atgcccgtat gaaagccgat ccc
//
LOCUS       DAZFKM010000597          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZFKM010000597 DAZFKM010000000
VERSION     DAZFKM010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..>453)
                     /locus_tag="ACHKAY_005938"
     CDS             complement(1..>453)
                     /locus_tag="ACHKAY_005938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO5704333.1"
                     /translation="PAGDAGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPA
                     GPQGPKGETGAAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSNGWFPGTDGALI
                     TGLTFLDPKDATQVQGLFRHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT           68 a          173 c          108 g          104 t
ORIGIN      
        1 tcattctcct gttctgcctg tatcactgcc cacttcatca agccccttaa catcctgcca
       61 cggcccgtca ccaaacctga cctgcaaatg ccgaaacagc ccctgaacct gtgtggcatc
      121 tttggggtca agaaaggtca gtccggtgat gagcgcacca tctgtacccg ggaaccagcc
      181 attgctgttt gtctcaataa tgctcgccgg ccccagacga aaacggattt gtgtctcccc
      241 cgggtcgccc tttggtccct gaggtccggt tgcccccacc gggccagccg cacctgtttc
      301 tcctttcggt ccctgtgggc cagccgggcc tgccgcaccg gtatctccct ttggcccctg
      361 tggacctgca tttcccgtca gaccggtctc tccccgctct cccctgtcgc ctttcggccc
      421 ctgcggacct gccggaccag catcacctgc cgg
//
LOCUS       DAZFKM010000598          452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZFKM010000598 DAZFKM010000000
VERSION     DAZFKM010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          143 a           91 c           82 g          136 t
ORIGIN      
        1 atacccctaa ttcaactaat tggaggcatt accatgcatt agtgcaagcg ccttgtggat
       61 tcttttgcta atggcggaaa catattgtac aatattgcat ttatccctca ataaataaat
      121 gagttatcag attgcaaaaa tctgttatca aactaattca aataaaaaga gattgatacg
      181 accaacgtac acaaaaattt tcgatattcg aactaaataa cagctcctaa taccgctaca
      241 ctttagctat ccagtctgcg cctcagaaat ggacgatgcc gttgaaggac tggtgtatgg
      301 caatgagccg ctttattatc gaattcggtg accgcttgga cggtcacttc ggagaaaagg
      361 catttacaca gcatcctaaa caggttcagc ataggtgata taccgccttt tcattttctt
      421 ctccattatt tataagggag gtgtattatt at
//
LOCUS       DAZFKM010000599          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZFKM010000599 DAZFKM010000000
VERSION     DAZFKM010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..>448)
                     /locus_tag="ACHKAY_005939"
     CDS             complement(32..>448)
                     /locus_tag="ACHKAY_005939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO5704334.1"
                     /translation="SRGRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKA
                     PAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYAT
                     IKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT           98 a          115 c          115 g          120 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggct
//
LOCUS       DAZFKM010000600          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZFKM010000600 DAZFKM010000000
VERSION     DAZFKM010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>448
                     /locus_tag="ACHKAY_005940"
     CDS             <1..>448
                     /locus_tag="ACHKAY_005940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO5704335.1"
                     /translation="GAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLAL
                     PENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIK
                     GYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKL"
BASE COUNT          166 a           83 c           78 g          121 t
ORIGIN      
        1 ggcgcttttt tatcggctat tcatcaccag atcattgaaa accaactgga tacagaaaca
       61 ataactatta gttatgaatt tatcctctgt caacatctta tcgcaaatac cgagttcagc
      121 tatttggcac tccctgaaaa ttataaccgt ttgtttttac caaattcaaa aaaccaaaca
      181 aataatcgtt tcaaaacgtt aaacagcaaa gctattggca ggctacttgc tgctggtggc
      241 gtatacaatg ggaacattga aggattcaga gatactgcgg aaaaactggg tggagatgca
      301 ataaaaggct atgatcaaat actaaatgaa aaaacagcgg gcatagcgat agcaacagca
      361 tctattcttt taacaaagcg ttctaatgtt gatacatata cagaaataaa tagttactta
      421 ggcaaactta gaggtcaaca aaaacttc
//
LOCUS       DAZFKM010000601          446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZFKM010000601 DAZFKM010000000
VERSION     DAZFKM010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..388
                     /locus_tag="ACHKAY_005941"
     CDS             <1..388
                     /locus_tag="ACHKAY_005941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO5704336.1"
                     /translation="DAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQ
                     ELKNARDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDI
                     IKAMNKAAALDELIPGLLSEYIEQSG"
BASE COUNT          112 a          112 c          134 g           88 t
ORIGIN      
        1 ggatgctgaa attgagaacg aaaagctgcg ccgggaagtt gaagaactgc ggcaggccag
       61 cgagacagat ctccagccag ggactattga gtacgaacgc catcgactta cgcgtgcgca
      121 ggccgacgca caggagctga aaaatgccag agactccgct gaagtggtgg aaaccgcatt
      181 ctgtactttc gtgctgtcgc ggatcgcagg tgaaattgcc agtattctcg acgggatccc
      241 cctgtcggtg cagcggcgtt ttccggaact ggaaaaccga catgttgatt tcctgaaacg
      301 ggatatcatc aaagccatga acaaagcagc cgcgctggat gaactgatac cggggttgct
      361 gagtgaatat atcgaacagt caggttaaca ggctgcggca ttttgtccgc gccgggcttc
      421 gctcactgtt caggccggag ccacag
//
LOCUS       DAZFKM010000602          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZFKM010000602 DAZFKM010000000
VERSION     DAZFKM010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            344..>434
                     /locus_tag="ACHKAY_005942"
     CDS             344..>434
                     /locus_tag="ACHKAY_005942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704337.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALD"
BASE COUNT          155 a          101 c           77 g          101 t
ORIGIN      
        1 cgttttcagc acctgtcgtt tcctttcttt tcagggggtg ttttaaataa aaacattagg
       61 ttacggcgaa gaagaacgga aacgccttaa accggaaaat tttcataaat agcgaaaacc
      121 cgcgaggtcg ccgccccgta acctgtcgga tcgccggaaa ggacccacga aaatgataat
      181 aattatcatc tacatgagat ttatcacgac atgtgtgtac gccatcaaac cacgagaaat
      241 aatcaattat tacgcaggta tcgtattaat tgatctgcat caaattagcg taaaagcaac
      301 ttcagataat acaaatcagc aacactgaat acggggcaac attatgtcat caaagaacag
      361 aacccgcaga acaacaaccc gcaacatccg atttccaaac cagataattg aacaaattaa
      421 catcgctctt gacc
//
LOCUS       DAZFKM010000603          433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZFKM010000603 DAZFKM010000000
VERSION     DAZFKM010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>433
                     /locus_tag="ACHKAY_005943"
     CDS             <1..>433
                     /locus_tag="ACHKAY_005943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFO5704338.1"
                     /translation="QEALEQQLTPSAPDVRLSAPGSFAHKINFPVETPCFQIKQTELK
                     GADALPHWLPLQKIANGAVGHCLGAKGINLLMSTLQNRLVDHGYVTTRVLAPSQDLKS
                     GILRLVIIPGVVRHVRLTPDSDDYIQLYSSFPAHEGSLLDLR"
BASE COUNT           95 a          110 c          109 g          119 t
ORIGIN      
        1 gtcaggaagc ccttgagcaa caactgacgc cttcagcccc tgatgttcgc ctttctgcac
       61 ctggctcttt tgcccataag attaattttc ctgttgaaac gccctgtttt cagattaaac
      121 agacggaact gaagggggct gatgcgttac cacactggct gcctttacaa aaaatcgcca
      181 acggggcggt cgggcattgc ctgggggcga aaggaattaa tctgctgatg agtacattgc
      241 agaaccgtct ggtcgatcat ggttatgtca ccacccgtgt tctggcacct tcgcaggatt
      301 taaaaagcgg tatcctccgg ctggttatta ttcccggtgt tgtgcgacat gtgcgtctga
      361 caccggacag tgatgactat attcagttgt attcctcatt cccggcacac gaaggttctc
      421 tgctggattt acg
//
LOCUS       DAZFKM010000604          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZFKM010000604 DAZFKM010000000
VERSION     DAZFKM010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHKAY_005944"
     CDS             <1..330
                     /locus_tag="ACHKAY_005944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5704339.1"
                     /translation="ATFSLRMKGKPVSFVVPLAFVKNLDKTLTVNTGALLTMSVSANG
                     GTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSAEKAQSVTSVECTV
                     TVSAAAG"
     gene            297..>424
                     /locus_tag="ACHKAY_005945"
     CDS             297..>424
                     /locus_tag="ACHKAY_005945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5704340.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
BASE COUNT          111 a           93 c          137 g           83 t
ORIGIN      
        1 gcaacgtttt cactgcgtat gaaaggcaaa ccggtgtcct ttgtggtacc gctggcgttt
       61 gtgaaaaatc tggataaaac acttaccgtg aatacaggtg cgctgctgac aatgtcagtc
      121 agtgccaacg ggggaacgcc gccgtataaa tacgcctgga agaaggatgg tcagccggtt
      181 gacgggcaga cgacagacac cttcagtaag ccaggtgcgc agtccgctga tgcggggaaa
      241 tatacctgcg tggtgaccga ttcggcagag aaagcacaga gtgtgacgtc tgttgaatgc
      301 accgtgacag tgagcgcagc cgccggataa ggggatgggt catcatgaaa aaggatctga
      361 aaacgctggc gctggccaga ctgtcagggt ttcgtcataa aacggtgaag gtgccggaat
      421 gggg
//
LOCUS       DAZFKM010000605          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZFKM010000605 DAZFKM010000000
VERSION     DAZFKM010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..156)
                     /locus_tag="ACHKAY_005946"
     CDS             complement(<1..156)
                     /locus_tag="ACHKAY_005946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleG8"
                     /protein_id="HFO5704341.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGF"
     gene            complement(224..>419)
                     /locus_tag="ACHKAY_005947"
     CDS             complement(224..>419)
                     /locus_tag="ACHKAY_005947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5704342.1"
                     /translation="KGIFVKNSDGSDVCTLFDAAAFSRLTGEGLPHPLTREPITASII
                     VKHEECIYDDTRGNFVIKGN"
BASE COUNT          130 a           83 c           77 g          129 t
ORIGIN      
        1 aaatccatcc agtagttgga catagtgaac agaatattgg ccactaccaa tggatattct
       61 tattccactt tcaagtctgg ctgcatcctg caaagactgc actcctgcag gagataattg
      121 agatatactt gggatactta aggtggtaac aggcataaaa acctcattca gtaattcata
      181 tcagtttgca ggatgtttca ttaaataaag ggtaatgttc atttcaatta ccctttataa
      241 cgaagtttcc tctggtatcg tcataaatgc attcttcatg ttttacaatt attgatgccg
      301 ttattggttc ccgggtcagt gggtggggta agccttcacc agtcaaacga gaaaatgcag
      361 cggcatcaaa taaagtacat acatctgaac catctgaatt cttcacaaaa atacctttt
//
LOCUS       DAZFKM010000606          419 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZFKM010000606 DAZFKM010000000
VERSION     DAZFKM010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..209
                     /locus_tag="ACHKAY_005948"
     CDS             <1..209
                     /locus_tag="ACHKAY_005948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO5704343.1"
                     /translation="IEVFLPAQVPDSELDSWMESRIYPAMSAIPALSGLITTMVTQGY
                     EYRRDDDMALWSSADLTYSITYEM"
     gene            217..>419
                     /locus_tag="ACHKAY_005949"
     CDS             217..>419
                     /locus_tag="ACHKAY_005949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO5704344.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWT"
BASE COUNT          103 a           95 c          123 g           98 t
ORIGIN      
        1 atattgaagt tttcctgcct gctcaggtgc cggattcaga gctggattcg tggatggaaa
       61 gccggattta tccggcgatg agtgcgatcc cggcactgtc aggcctgatt accacgatgg
      121 ttacgcaggg ctatgagtat cgtcgtgatg acgatatggc gttatggagt tctgctgatt
      181 tgacttattc cattacatac gagatgtgag gacgatatgc caacaccaaa tcctctggca
      241 ccggtaaaag gtgccggtac caccctgtgg gtttataacg gtcagggtga tgcctatgca
      301 aacccgttgt cagacgataa ctggcagcga ctggcacagg taaaggatct gacgccgggc
      361 gagatgacgg cagaacccta cgatgataac tacctggatg atgaagacgc ggactggac
//
LOCUS       DAZFKM010000607          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZFKM010000607 DAZFKM010000000
VERSION     DAZFKM010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>416
                     /locus_tag="ACHKAY_005950"
     CDS             <1..>416
                     /locus_tag="ACHKAY_005950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5704345.1"
                     /translation="HPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEV
                     VRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDE
                     LSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAG"
BASE COUNT          108 a          115 c          114 g           79 t
ORIGIN      
        1 ccatcctgat gcggttgtat tcagccctaa aaagcgccag tggggaacgc aggatgattt
       61 gacctgcgca cagtggctct ggaaaaaaat catcgccctg tacgagcaag ccgccgaatg
      121 tgacggcgag gtggttcgtc ccaaagaacc gaactggaca gcctgggcaa acgaaattcg
      181 cctgatgtgt gtgcaggatg gtcgtactca caaacaaatc tgcgagatgt acagccgcgt
      241 cagccgcgat ccgttctggt gccgtaacgt gctcagcccg tcgaagttgc gggaaaaatg
      301 ggatgagctt tccctgcgct tatcaccgtc cgtcagcacg tacaccgaaa aacgcgaaga
      361 cccgtacttc aaatccagtt acgacaacgt ggactacagc cagatcccgg caggat
//
LOCUS       DAZFKM010000608          415 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZFKM010000608 DAZFKM010000000
VERSION     DAZFKM010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..330
                     /locus_tag="ACHKAY_005951"
     CDS             <1..330
                     /locus_tag="ACHKAY_005951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO5704346.1"
                     /translation="ATFSLRLKGKLVSYVVPLAFVKNLEKTLTVNTGALLTMSVSVNG
                     GTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSAEQPQSITSDACTV
                     TVNGAGG"
     gene            336..>415
                     /locus_tag="ACHKAY_005952"
     CDS             336..>415
                     /locus_tag="ACHKAY_005952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO5704347.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEW"
BASE COUNT          111 a           89 c          127 g           88 t
ORIGIN      
        1 gcaacgtttt cacttcgcct gaagggtaaa ctggtgtcct atgtggtacc gctggcgttt
       61 gtgaaaaatc tggagaagac acttaccgtg aataccggtg cgctgctgac aatgtcagtc
      121 agtgtcaacg ggggaacgcc gccttataaa cacgcctgga agaaggatgg tcagccggta
      181 gagggacaga ctactgacac tttcagtaaa gccaatacgc agtcaggtga taagggggct
      241 tatacctgcg aggtaacgga ttctgcagaa cagccgcaga gcattacctc tgatgcgtgt
      301 acagtaacgg ttaatggtgc gggcggataa ggcttatggc aaaagatctg aaaacactgg
      361 cgctggccag actgtcgggg ttccgtcata aaacggtgaa ggtgccggaa tggag
//
LOCUS       DAZFKM010000609          414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZFKM010000609 DAZFKM010000000
VERSION     DAZFKM010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHKAY_005953"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHKAY_005953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT           97 a          110 c           86 g          121 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atct
//
LOCUS       DAZFKM010000610          413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZFKM010000610 DAZFKM010000000
VERSION     DAZFKM010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>413
                     /locus_tag="ACHKAY_005954"
     CDS             <1..>413
                     /locus_tag="ACHKAY_005954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5704348.1"
                     /translation="REYDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQ
                     MTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQ
                     GRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKA"
BASE COUNT           98 a          119 c          137 g           59 t
ORIGIN      
        1 ccgggaatat gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt
       61 acgctaccgc tacgatgacg cgcacagtga gttaccggcg acgacaacgg atgcgacggg
      121 cagcacccgg cagatgacgt ggagccgcta cgggcagttg ctggcgttca ctgactgctc
      181 gggctaccag acccgctatg aatacgaccg cttcggccag atgacggcgg ttcaccgcga
      241 ggaaggtatc agcctttacc gccactatga caaccgtggc cggttaacct cggtgaaaga
      301 cgcacagggc cgtgaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
      361 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cgg
//
LOCUS       DAZFKM010000611          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZFKM010000611 DAZFKM010000000
VERSION     DAZFKM010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>411)
                     /locus_tag="ACHKAY_005955"
     CDS             complement(<1..>411)
                     /locus_tag="ACHKAY_005955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO5704349.1"
                     /translation="PDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPHEYTFLDGGGLH
                     FLRWELMEVSAVTVPANAECTIRTIKSYDRPFSAASGNRKPVVKIASSAGAAAQSTTV
                     FHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKA"
BASE COUNT           86 a          120 c          114 g           91 t
ORIGIN      
        1 ggctttggtc atgacctcct caagggaggc tgccagcgct gcacgcttgt tttcaaaact
       61 tttaatctgt tcgccaatat tcattatggt cttttcctta tgaaaaacgg ttgttgactg
      121 tgccgcagcg ccggcagaag atgcgatttt caccaccggt ttccggttgc cggacgcggc
      181 agaaaacggg cggtcgtaag atttaatggt ccggatggtg cattccgcat tcgcgggcac
      241 ggtgacggca gacacctcca tcagttccca gcgcagaaaa tgcagtccgc ctccgtccag
      301 aaaggtgtat tcatggggac ggaagcccac ggacagcccc ctgaccagcc cggtcttaat
      361 ggccgcccag acctcatcca gccgggcagc cagttgcgac ggcatatccg g
//
LOCUS       DAZFKM010000612          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZFKM010000612 DAZFKM010000000
VERSION     DAZFKM010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.618"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHKAY_005956"
     CDS             complement(<1..348)
                     /locus_tag="ACHKAY_005956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO5704350.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEA"
BASE COUNT          133 a          109 c           67 g          100 t
ORIGIN      
        1 agcctcaagc gggcactgaa gtctctctgg acaaacagaa aacttacatt gagatatttt
       61 gttctgaata ctctcttgcg attgtgcttc acctgagtcc gaaagcatac ttagcattct
      121 ctcaccaagg gctcgaggac cactattaaa tcccaaaagc aataactcag aaagcaatcc
      181 gccattaccg ccactggatg aaaagcgccc attagtagaa tcatacacaa tactgacagt
      241 ttcaccaccg actgtaatat ctccgcgccc attggctaca attcgggcac gaacaacctc
      301 caccccaaga ttaaatgaat gtgatctaat atctgaggtt aatggcataa caaagctccc
      361 tattttaatt tgaactccag acttaaatag ctgtaacaaa catccccca
//
LOCUS       DAZFKM010000613          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZFKM010000613 DAZFKM010000000
VERSION     DAZFKM010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..377
                     /locus_tag="ACHKAY_005957"
     CDS             <1..377
                     /locus_tag="ACHKAY_005957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO5704351.1"
                     /translation="KRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRM
                     RKGNVPLNYNVHHKLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKI
                     TPWAIPSGSIYPPMKNIMDHTK"
BASE COUNT          163 a           73 c           60 g          110 t
ORIGIN      
        1 ttaagagacc ttcaaaagac ttagctaact tacgaaagga gtttaataaa actgtaagaa
       61 aaaattttct tatcaaactt gcaaaaacct ccgaagcatc tggaagattc aacgccgaag
      121 accttttaag aatgagaaag ggcaatgttc ctctaaatta taatgttcac cataaactat
      181 ctctagatga tggtggtact aatgatttcg aaaatttagt attaatcgaa aacgaaccat
      241 atcataaagt ttttactaac atgcaatcac gaatagctaa gggaatatta gtaggtgaaa
      301 gcaaaatcac tccctgggcc attccatctg gctcaattta tcctcccatg aaaaatatta
      361 tggaccacac aaaatgatga ctaaaaccca aataaataaa ttaata
//
LOCUS       DAZFKM010000614          404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZFKM010000614 DAZFKM010000000
VERSION     DAZFKM010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>404)
                     /locus_tag="ACHKAY_005958"
     CDS             complement(<1..>404)
                     /locus_tag="ACHKAY_005958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704352.1"
                     /translation="EAIVDNGAHFDGDQSGTLNSVIPPAVQHLTVEVSAADGQYLAQA
                     KWDTPRVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNA
                     WGQQGDPASVSFRIAAPAAPSRIELTPGYFQIT"
BASE COUNT           64 a          141 c          115 g           84 t
ORIGIN      
        1 gttatctgaa aatagcccgg tgtcagctca atccgagacg gcgctgccgg tgcggcaatc
       61 cggaacgata ccgatgccgg atcgccctgc tgtccccacg catttaccgc ccggactgtc
      121 aacctgtagt tccccagcgc cagttgcgtg aagcggtatg tggtttctgt cgtcctggcc
      181 gtgctgacca gccgctcact gccgtcatcc gctgccacgg tcaggcgaag caggaagctc
      241 acgcccttca ccacccgcgg cgtgtcccat ttcgcctgcg ccagatactg gccgtcagct
      301 gcgctcacct ccaccgtcag gtgctgcact gccggaggga tgacgctgtt cagggtgccg
      361 ctctggtcgc cgtcaaagtg cgctccgtta tccacgatgg cttc
//
LOCUS       DAZFKM010000615          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZFKM010000615 DAZFKM010000000
VERSION     DAZFKM010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..383
                     /locus_tag="ACHKAY_005959"
     CDS             60..383
                     /locus_tag="ACHKAY_005959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO5704353.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          154 a           67 c           64 g          118 t
ORIGIN      
        1 aataaagaaa tgaacatcca aatgaactat attaaatacc gtgggataag acataacaaa
       61 tgaagtggat agtaattgac acgataattc aaccttcatg cggcatatct ttttcagcca
      121 tatggggtaa tatgaaaatg atcatctggt atcaatctac tatatttctc cctcctggca
      181 gtatatttac accggttaaa tctggaatta tccttaaaga taaagaatat cctattacta
      241 tttataacat cgcaccattc aacaaggatt tatggagtct actcaaaagc agtcaagagt
      301 gtcctccagg agaaagcaaa ataacaaata aatgtttgca taatagttgc attataaaaa
      361 tatgcccata tgggctcaag taaagggttt tatataaata tgc
//
LOCUS       DAZFKM010000616          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZFKM010000616 DAZFKM010000000
VERSION     DAZFKM010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..320)
                     /gene="bhsA"
                     /locus_tag="ACHKAY_005960"
                     /gene_synonym="ycfR"
     CDS             complement(63..320)
                     /gene="bhsA"
                     /locus_tag="ACHKAY_005960"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO5704354.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          104 a           89 c           98 g          112 t
ORIGIN      
        1 ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat gagggttaat
       61 gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac agaagtaata
      121 cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc cagcgatccc
      181 agatttgtcc ccgcgttagc actgattgta ccgacttttt gttggccttc tggcgttgac
      241 tgaacttcga cagccgcaaa gctggcaaat gacatggagc ttaaaatcgc cgcagcgatg
      301 agggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt
      361 acttaacgat cgataactaa atgatagatg tgatctggat cac
//
LOCUS       DAZFKM010000617          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZFKM010000617 DAZFKM010000000
VERSION     DAZFKM010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>395)
                     /locus_tag="ACHKAY_005961"
     CDS             complement(<1..>395)
                     /locus_tag="ACHKAY_005961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO5704355.1"
                     /translation="INTLESFKKPQDYFFYQQEMLLRWNYAAASDQVRMNILKEYGGI
                     YTDTDILPAYSDEVSQIINKKSDGDMFFEDLKLRRFISEAILSLIKGEKYSIKHDSLD
                     EKTRNQLNAILSEIEKLTIDNYFKPVETT"
BASE COUNT          116 a           72 c           49 g          158 t
ORIGIN      
        1 ctgttgtctc aacaggttta aaataattat ctatcgtcag cttttctatt tcactcaata
       61 tagcatttaa ctgattacgt gttttttcat ctaagctatc atgcttaatt gagtactttt
      121 ccccttttat gagggacaga atagcttctg atataaacct tcttagtttt aaatcttcaa
      181 aaaacatatc tccatcactt tttttattta ttatctgtga cacttcatct gaatatgcag
      241 gaagaatatc agtatcggta taaatccctc catactcttt caatatattc attcttacct
      301 gatctgatgc agcggcgtag ttccatctta atagcatctc ttgttgataa aaaaaataat
      361 cttgtggttt tttaaatgat tccaatgtgt taata
//
LOCUS       DAZFKM010000618          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZFKM010000618 DAZFKM010000000
VERSION     DAZFKM010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>390
                     /locus_tag="ACHKAY_005962"
     CDS             <1..>390
                     /locus_tag="ACHKAY_005962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704356.1"
                     /translation="GVDVDEWMRSLKRHERRALMRSLRDKQAKNSSDEMQSWTQSRKQ
                     QRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYVDGSIIAPQGFEIVRKP
                     DTRPDSRITQLWQRLSRNHGVSSTEIRH"
BASE COUNT          103 a          104 c          114 g           69 t
ORIGIN      
        1 ggcgttgatg tggatgaatg gatgcgctca ctgaaacggc acgaacgccg ggcgctgatg
       61 cgttcgcttc gtgacaaaca ggcaaaaaac agcagtgatg aaatgcagag ctggacacag
      121 agccgcaaac agcagcggcc tttgcctgat aaccacgaat tactcgctaa agaatggcgg
      181 gagtctgctg aatctctcgg cctgcatatc ggtgaacaac agatgcagca cctgttacgg
      241 ggcggcagtc tgtacgttga cggcagcatc attgcaccgc agggatttga aattgtacgc
      301 aaaccggata cccgcccgga cagccgaatc acgcagctct ggcagcgcct gagccgtaat
      361 catggcgtaa gcagcacgga gatccgccat
//
LOCUS       DAZFKM010000619          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZFKM010000619 DAZFKM010000000
VERSION     DAZFKM010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          120 a           72 c           92 g          106 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgactgac ctgaaaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgcct tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcaaaaggat agatgtactt
      241 cacatgttgc ttctatatgg cagtacattt tctgacagac agtgacggat gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg aggccggaat gttatctggc atttttagca
      361 gagcctgaat gccataatca cggctcccgg
//
LOCUS       DAZFKM010000620          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZFKM010000620 DAZFKM010000000
VERSION     DAZFKM010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>388)
                     /locus_tag="ACHKAY_005963"
     CDS             complement(<1..>388)
                     /locus_tag="ACHKAY_005963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO5704357.1"
                     /translation="NAFGAAKFSGILKNIAMNYAIMRLRRTNPHLAEKMNDVILKIQH
                     IQNETVDFNETRERVKELENKYKSLTRETKEKFNVFFLESMIGMQDNYFTYCISNGIS
                     NTDDISRLNFLTTVLKLSPDILDDFKS"
BASE COUNT          116 a           60 c           52 g          160 t
ORIGIN      
        1 tagatttaaa gtcatccaga atgtctggtg aaagttttaa aacagtagtt aaaaagttaa
       61 gtctggatat atcatcagta tttgatattc cgtttgatat acaatatgtg aaatagttgt
      121 cttgcatacc tatcattgac tcaagaaaaa aaacattgaa tttttctttg gtttctctcg
      181 ttaatgattt gtatttattt tcaagttcct ttaccctctc tcttgtttca ttaaaatcta
      241 ctgtttcatt ttgtatgtgt tgtattttta atatcacatc attcattttt tccgctaaat
      301 gaggattggt tcttctcaaa cgcataattg cataattcat ggctatattt tttaaaattc
      361 cactaaactt ggctgcacca aaagcatt
//
LOCUS       DAZFKM010000621          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZFKM010000621 DAZFKM010000000
VERSION     DAZFKM010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..225)
                     /gene="tnpB"
                     /locus_tag="ACHKAY_005964"
     CDS             complement(<1..225)
                     /gene="tnpB"
                     /locus_tag="ACHKAY_005964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO5704358.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGR"
BASE COUNT           83 a          103 c          109 g           89 t
ORIGIN      
        1 gcggccgcgc tccagccgtt tggtcagcag acacagtcca tcgccggtag accagaggag
       61 ctttacctga ctgccattac gcccacggaa gataaaaacg tgacctgaca tcggatcgtc
      121 tttcagcgtc gtctgcacct ttgccgccag gccgttgaag ccgtttctca tatcggtgat
      181 gccagcgacc agccagatct ttgtccctga tggtaatggg atcaacgttt gagctcccgt
      241 atcagcaggt tcaggatatt ttcgctgatg gcaccattca gacggagtga tccgtgccgg
      301 aacgtcactt cacagctgat attgagtgat tcaggtgtcg cagcagctac aggttgtgac
      361 cggggggagg ttgtagggac aaca
//
LOCUS       DAZFKM010000622          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZFKM010000622 DAZFKM010000000
VERSION     DAZFKM010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..354
                     /locus_tag="ACHKAY_005965"
                     /pseudo
     CDS             1..354
                     /locus_tag="ACHKAY_005965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          125 a           59 c           73 g          125 t
ORIGIN      
        1 atgttttcta caaacttaat acagagtaat tatggtgatt taaatattaa aactctggct
       61 tttgactctt ttaaagaaag gcttgagtct accatggcgg cactcacttt ctttatatca
      121 acgggtcaat gtgactgcga tgaaatagca gaatcaaact tcaatcgtat gattgcctat
      181 atgtctaaca ttaactatga tgcctcaaag ccaggagcgc cagcacttag ttttgataca
      241 tatctgcagg acaatgttaa atatagagta ataattaata atctttatgg cggtgagttt
      301 cggataagag atattaatga gaattttgtt gggatggatt ttacctgtga attctgaccc
      361 gaaaaaatga cgaatttgat ca
//
LOCUS       DAZFKM010000623          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZFKM010000623 DAZFKM010000000
VERSION     DAZFKM010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>381
                     /locus_tag="ACHKAY_005966"
     CDS             <1..>381
                     /locus_tag="ACHKAY_005966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO5704359.1"
                     /translation="VIYDAGYKNYHESLNAAIAINSRPLQEIHYGDVLYHYHKNDISL
                     GVDTLYGRESFDIVLDAMNVYRKSKKMRVISNNEMKKSIKISELVIHNIIKKGLTNCL
                     LKKDVLNARYDLIRDILRYSLNIR"
BASE COUNT          148 a           42 c           64 g          127 t
ORIGIN      
        1 tagtaatata tgatgcgggt tacaaaaatt atcatgagtc attaaatgct gctattgcaa
       61 taaactctag accattacaa gaaatacatt atggtgatgt tttatatcat tatcataaaa
      121 atgacatctc tttgggagta gatactcttt acgggaggga aagttttgat attgtactgg
      181 atgcaatgaa cgtatataga aaaagcaaaa aaatgagagt tatttccaat aatgagatga
      241 aaaaaagcat taaaatatct gaattagtta tccataatat tataaagaaa ggattgacta
      301 attgtttgct taaaaaggat gttcttaatg ccagatatga tcttattaga gatattcttc
      361 gatattcttt aaatatacga c
//
LOCUS       DAZFKM010000624          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZFKM010000624 DAZFKM010000000
VERSION     DAZFKM010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHKAY_005967"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHKAY_005967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO5704360.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVG"
BASE COUNT          103 a          105 c           78 g           95 t
ORIGIN      
        1 tctccgacca gaagcgtaaa tcccagtacc gtcaaacaca gcactattac tgccacaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccgagtt
      121 gttgaggctc ggagtggcag ccccaggatt gatagaaata tctcctgggg cttcatcttt
      181 ccagacctca gagttaacct gaaaccggaa agcttcaggc acccgccgtt atcttaccct
      241 tcctcaacaa aatatctgtt tatttataca gtgaatcctg tttcggactt ctgtcggcaa
      301 aactcatcat tgctcttaaa acgaaatcgc ccgcagccat cactgaccgc gggcgaaagt
      361 gtaaagcagg tgccttacca t
//
LOCUS       DAZFKM010000625          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZFKM010000625 DAZFKM010000000
VERSION     DAZFKM010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..>374
                     /locus_tag="ACHKAY_005968"
     CDS             183..>374
                     /locus_tag="ACHKAY_005968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5704361.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           93 a           94 c           93 g           94 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacc
//
LOCUS       DAZFKM010000626          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZFKM010000626 DAZFKM010000000
VERSION     DAZFKM010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>369)
                     /locus_tag="ACHKAY_005969"
     CDS             complement(<1..>369)
                     /locus_tag="ACHKAY_005969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5704362.1"
                     /translation="LRLAVRLPGRVTTSRLLHTATGAVAHVTRELEAWQKEQQAAGHQ
                     TLADVPAPVINGESVNLWHWRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSD
                     LWRDVSWAISDILCRPRIFAE"
BASE COUNT           63 a          118 c          115 g           73 t
ORIGIN      
        1 ctccgcaaag attcgcgggc ggcacagaat gtcagagatg gcccagctca catcgcgcca
       61 caaatccgat gtctgtatat ccagtgcgtc cgcccggcgg tcgcccttgt ccgttgtgtc
      121 cgcatcgcgg taacgctcca gaatcagggc gcgtgtggcg gtgtaaaccg cattgcgcca
      181 gtgccagaga ttgacgcttt ctccgttaat tacgggtgcc ggaacatcgg ccagcgtctg
      241 atggccagcc gcctgctgtt ccttctgcca tgcttccagc tcgcgggtaa cgtgtgccac
      301 agctccggtg gcggtatgca gcaggcggga ggtggtcacg cgccccggca gtcgtaccgc
      361 cagacgcag
//
LOCUS       DAZFKM010000627          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZFKM010000627 DAZFKM010000000
VERSION     DAZFKM010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>369)
                     /locus_tag="ACHKAY_005970"
     CDS             complement(<1..>369)
                     /locus_tag="ACHKAY_005970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO5704363.1"
                     /translation="LRLAIRLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQ
                     TLADVPAPVINGESVNLWHWRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSD
                     LWRDVSWAISDILRRPRIFAE"
BASE COUNT           64 a          117 c          118 g           70 t
ORIGIN      
        1 ctccgcaaag attcgcgggc ggcgcagaat gtcagagatg gcccagctca catcgcgcca
       61 caaatccgat gtctgtatat ccagtgcgtc cgcccggcgg tcgcccttat ccgttgtgtc
      121 cgcatcgcgg taacgctcca gaatcagggc gcgtgtggcg gtataaacag cattgcgcca
      181 gtgccagaga ttgacgcttt ctccgttaat tacgggggcc ggaacatcgg ccagcgtctg
      241 atggccagcc gcctgctgtt cctgctgcca cgcttccagc tcgcgggtaa cgtgtgccac
      301 ggccccggtg gcggtatgca gcaggcggga ggtggtcacg cggcccggca gtcgtatcgc
      361 cagacgcag
//
LOCUS       DAZFKM010000628          365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZFKM010000628 DAZFKM010000000
VERSION     DAZFKM010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>365)
                     /locus_tag="ACHKAY_005971"
     CDS             complement(<1..>365)
                     /locus_tag="ACHKAY_005971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5704364.1"
                     /translation="LVGATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMS
                     QLTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTD
                     IRKDRCSKCMRGCELRRNVG"
BASE COUNT           72 a          137 c           90 g           66 t
ORIGIN      
        1 ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc cttacggata
       61 tccgtggtgg ggttgtcgaa ctcatccgcc accgcaggac cgttataccc gcattcatct
      121 ccccggtaat cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgcccca
      181 tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag ctgcgacatc
      241 tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg attgcccgca
      301 acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac cgtggccccc
      361 accag
//
LOCUS       DAZFKM010000629          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZFKM010000629 DAZFKM010000000
VERSION     DAZFKM010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>364)
                     /locus_tag="ACHKAY_005972"
     CDS             complement(<1..>364)
                     /locus_tag="ACHKAY_005972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO5704365.1"
                     /translation="IKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDL
                     VVGDGDKKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKY
                     RKNAVWVVNSTTAAKLQKV"
BASE COUNT           82 a           97 c           84 g          101 t
ORIGIN      
        1 accttctgaa gttttgccgc cgtcgtggag ttaaccaccc agaccgcgtt tttgcggtat
       61 tttttacgta atgcaaattt caggtcgatc agcgggtccg cagatgtcca cgccagagat
      121 tcggaaggtt taattacctg caacgtacca aaatcacgct ctttgtcgtt cttctctgca
      181 cggggtacgg ataaaaaacc ttttgctttt ttgtcaccgt cgcccacaac cagatcgctt
      241 tcttcggttt ccgtgaaggt gtcgccaatc tcacccgtca gccatgaaag gatgtccaca
      301 tcggaaaaat ccacgatttc ctgtgtggtg cgcgggtacg catagaccgg atacagctta
      361 atgc
//
LOCUS       DAZFKM010000630          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZFKM010000630 DAZFKM010000000
VERSION     DAZFKM010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>364
                     /locus_tag="ACHKAY_005973"
     CDS             <1..>364
                     /locus_tag="ACHKAY_005973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO5704366.1"
                     /translation="SPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSENSGESS
                     DQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIR
                     LMRERDGRNHRDMCVLFRW"
BASE COUNT          114 a           85 c           93 g           72 t
ORIGIN      
        1 tcccctaaaa cgagggataa aacatccctc aaattagggg attgctatcc ctcaaaacag
       61 ggggacacaa aagacactat tacaaaagaa aaaagaaaag attattcgtc cgagaattct
      121 ggcgaatcct ctgaccagcc agaaaacgat ctttctgtgg ttaaaccgga tgctgcaatt
      181 cagagcggca gcaagtgggg aacagcagaa gacctgaccg ccgcagagtg gatgtttgac
      241 atggtgaaga ccatcgcacc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
      301 atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg atatgtgtgt gcttttccgc
      361 tggg
//
LOCUS       DAZFKM010000631          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZFKM010000631 DAZFKM010000000
VERSION     DAZFKM010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..68)
                     /locus_tag="ACHKAY_005974"
     CDS             complement(<1..68)
                     /locus_tag="ACHKAY_005974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704367.1"
                     /translation="MVTVAELQALRQARLDLLTGKRV"
     gene            complement(52..>359)
                     /locus_tag="ACHKAY_005975"
     CDS             complement(52..>359)
                     /locus_tag="ACHKAY_005975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO5704368.1"
                     /translation="YAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWDNKKRR
                     NEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVNGYSR"
BASE COUNT           72 a          118 c           81 g           88 t
ORIGIN      
        1 acccgtttac cggttaataa atcaaggcgc gcctgacgca gcgcctgcag ttcagcgact
       61 gtaaccattc actcctccgg acagcttcgc tgccagttct ttcagggttg gccgggtcgt
      121 ctcttcttcc cgggattttg ccagtacagc cagatcaagc tgccagcgtt gcacggacac
      181 acgtaatgcc gcgtaggcat acaccaggca gtccagcgct tcgttacgcc gctttttgtt
      241 atcccacagc agacgcatct ttcctttttc ccacttctcc accagctctt ccgccaccag
      301 ttgctgcgcc tctgtctgcg aaaaaatctc cggatcatca ggaaaacgga tggcatacg
//
LOCUS       DAZFKM010000632          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZFKM010000632 DAZFKM010000000
VERSION     DAZFKM010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHKAY_005976"
     CDS             <1..>356
                     /locus_tag="ACHKAY_005976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5704369.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZFKM010000633          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZFKM010000633 DAZFKM010000000
VERSION     DAZFKM010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..162
                     /locus_tag="ACHKAY_005977"
     CDS             <1..162
                     /locus_tag="ACHKAY_005977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO5704370.1"
                     /translation="AVCCLFDFDAFSRLASEGSYHPLTREPITASMIISPDKCVYDPI
                     KGNFIIKDS"
BASE COUNT          114 a           52 c           74 g          115 t
ORIGIN      
        1 gcagtatgct gcttatttga ttttgatgca ttttctcgtt tagctagtga aggctcatat
       61 catccactga cccgagaacc aataacggca tcaatgatta taagtcctga taaatgtgtt
      121 tatgatccta tcaagggaaa cttcattata aaagatagtt aaaatatttt tctctgagag
      181 agtattggct gtcagtaatt tgtaaaaaaa cgccgcagac atccagaata taagaaggtg
      241 tctgtggttg gcaggtttca gatgacttga aggcgtcttt atgtaaatgc atttttctgt
      301 ggagatgttg tcggaaaata ttggattaaa aacataatcc aagtcaaata gtaag
//
LOCUS       DAZFKM010000634          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZFKM010000634 DAZFKM010000000
VERSION     DAZFKM010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>355
                     /locus_tag="ACHKAY_005978"
     CDS             <1..>355
                     /locus_tag="ACHKAY_005978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO5704371.1"
                     /translation="FRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGT
                     IDEPGHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAI
                     QATKAAAAIRKITIEA"
BASE COUNT           89 a           91 c           86 g           89 t
ORIGIN      
        1 gtttccgtgg tgcgctttcc actgccagca tcgatgactt tacccgttat tctaaagatc
       61 ttgcagatga aggcacccgc tgctttatcg atgccgataa tatgcgtgcc gtcagtgtgc
      121 ttaacctggg tactattgat gaaccaggtc acgcagataa caccgccact ctcaaactga
      181 aaaagacagc accgttctct gctctgttgt ctgttaatgg cgagcgtaac tcccaaaagt
      241 cactggcaga atggattgaa gactgggccg actaccttgt gggctttgat gctaatggtg
      301 acgccattca ggcaaccaaa gcggctgcgg cgatccgtaa aatcacaatt gaagc
//
LOCUS       DAZFKM010000635          352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZFKM010000635 DAZFKM010000000
VERSION     DAZFKM010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          109 a           72 c           67 g          104 t
ORIGIN      
        1 aaatccccgt ccccagagaa ataatattta ttaaaatggc aggtctgctt tttctgatta
       61 ccgaagacaa agtggcaatt acgtatagtt tcccgataaa gacgcgacag ctacatcccg
      121 cataaggcat ttttctcttt atctttgtac ggtacttcat ggaacagaat ttttgacctt
      181 gtgaatcgtg atgtctgttg gggaggagca atttgctcac tgaagcgtga gactcgatta
      241 agcgcacgaa acacagaaat caaaaaaccc ggccactttt ttacaaactg accgggtaaa
      301 aataattttt attttttaac tgccttgaga ctcatagaga tgtctcaaaa ct
//
LOCUS       DAZFKM010000636          347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZFKM010000636 DAZFKM010000000
VERSION     DAZFKM010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>347)
                     /locus_tag="ACHKAY_005979"
     CDS             complement(<1..>347)
                     /locus_tag="ACHKAY_005979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO5704372.1"
                     /translation="GMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAAIERI
                     EVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSS
                     GPLVDDSVSLQVRG"
BASE COUNT           85 a          103 c           80 g           79 t
ORIGIN      
        1 ccgcgtacct gcaggctgac agaatcatcc acaaggggac cactgctcca gaaattaaac
       61 tggctgctgt taccccattt gttgctttcc tgcagattca gccctgcatt gacggaagag
      121 agccatttgt ctgcattctt tctggtaatg atattcacca caccgcccat cgcatcagag
      181 ccatacagtg tggacatcgg ccccctgata acctcaatac gctcaatggc ggccagaggg
      241 ggcatgaacc cggtattcat ggcagaaaaa ccgttgggag tcacgtcact gcttccgccc
      301 tgacgaacac catcaatcag tatcagcgtg taactggctg gcattcc
//
LOCUS       DAZFKM010000637          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZFKM010000637 DAZFKM010000000
VERSION     DAZFKM010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>346
                     /locus_tag="ACHKAY_005980"
     CDS             <1..>346
                     /locus_tag="ACHKAY_005980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO5704373.1"
                     /translation="ALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDE
                     PGHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQAT
                     KAAAAVRKITIEA"
BASE COUNT           90 a           89 c           83 g           84 t
ORIGIN      
        1 gtgcgctttc cactgccagc atcgatgact ttacccgtta ttctaaagat cttgcagatg
       61 aaggcacccg ctgctttatc gatgccgata atatgcgagc cgtcagtgtg cttaacctgg
      121 gtactattga tgaaccaggt cacgcagata acaccgccac cctcaaactg aaaaagacag
      181 caccgttttc tgctctgttg tctgttaatg gcgagcgtaa ctcccagaag tcactggcag
      241 aatggattga agactgggcc gactaccttg tgggctttga tgctaatggt gacaccattc
      301 aggcaaccaa agcggctgcg gcagtccgta aaatcacaat tgaagc
//
LOCUS       DAZFKM010000638          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZFKM010000638 DAZFKM010000000
VERSION     DAZFKM010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>344)
                     /locus_tag="ACHKAY_005981"
     CDS             complement(<1..>344)
                     /locus_tag="ACHKAY_005981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO5704374.1"
                     /translation="MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNW
                     RYCITDAYTGNTRNYLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVM
                     RDGMMAAGVITSG"
BASE COUNT           76 a          104 c           96 g           68 t
ORIGIN      
        1 cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt
       61 tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga gcgccccata
      121 aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata gttgcgggtg
      181 tttccggtgt aggcgtcagt gatgcagtag cgccagttgt gttcgcctgt ctgctccacg
      241 ttcaccgact gcggcgggta aggatgtaaa gccaccggaa aaccatcacg cccccactga
      301 atcaccgcat aagcattacc gtttaacagg cagtggcgga tcat
//
LOCUS       DAZFKM010000639          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZFKM010000639 DAZFKM010000000
VERSION     DAZFKM010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..288)
                     /locus_tag="ACHKAY_005982"
     CDS             complement(<1..288)
                     /locus_tag="ACHKAY_005982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO5704375.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
BASE COUNT           69 a          106 c           68 g          101 t
ORIGIN      
        1 tcttttaact ccatataccg ccaatacccg tttcatcgcg gcactctggc gacactcctt
       61 aaaaattagg ttcgtgctca tctttccttc ccgttcttcc ttggtagcaa accggtaata
      121 caccgttcgc cagaccttac cttcgataac cagaagacct gcccgtgcca ttttagccgc
      181 ggcctgattt atgctggtta ctgttgcgcc tgttagcgcg gcaacgtccg gcgcacagaa
      241 gctattatgc gtccccaggt aatgaataat tgcctctttg cccgtcatac acttgctcct
      301 ttcagtccga acttagcttt gagttctgcg atcttcgcca gagc
//
LOCUS       DAZFKM010000640          343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZFKM010000640 DAZFKM010000000
VERSION     DAZFKM010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..214
                     /gene="escF"
                     /locus_tag="ACHKAY_005983"
     CDS             <1..214
                     /gene="escF"
                     /locus_tag="ACHKAY_005983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO5704376.1"
                     /translation="SEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAVQQY
                     SAYVNVESGMLKTIKDLVSTISNRSF"
     gene            220..>343
                     /locus_tag="ACHKAY_005984"
     CDS             220..>343
                     /locus_tag="ACHKAY_005984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO5704377.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDK"
BASE COUNT          108 a           61 c           73 g          101 t
ORIGIN      
        1 atctgaaatt actcaacaaa tgggtgaagt aggtaaaacg ctgagtgatt ctgtgccaga
       61 gttacttaat agcaccgatt tggttaatga ccctgaaaaa atgttagagc tgcagttcgc
      121 ggttcagcaa tattctgctt atgttaacgt agaaagtgga atgctgaaaa cgataaaaga
      181 tttggtctca accatttcca accgtagctt ttaattttta tggttaatga tgtttctgcc
      241 aacaagatac tggtgtgggc ggctgtagct gcggcaaatc ataagcttcc caaatatgca
      301 gaatctatcc tgaatgtatt gccgcaaatt atacccgata aaa
//
LOCUS       DAZFKM010000641          343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZFKM010000641 DAZFKM010000000
VERSION     DAZFKM010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            226..343
                     /locus_tag="ACHKAY_005985"
                     /pseudo
     CDS             226..343
                     /locus_tag="ACHKAY_005985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           75 a          102 c           65 g          101 t
ORIGIN      
        1 tgatgatctt tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct
       61 cactaccatg gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag
      121 atcccggtgg gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc
      181 cgttttgcat ctttgtgcct acggcatccc tgtgatcttt ccctgaatct gcccctgaga
      241 acctgaatca aacgtctgac tcgcagaacc atctgtttct cgcgctctgt agaacttcat
      301 gaaaaagtca tcggtgcttt tatcgaaaaa tacatgttct act
//
LOCUS       DAZFKM010000642          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZFKM010000642 DAZFKM010000000
VERSION     DAZFKM010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..>340
                     /locus_tag="ACHKAY_005986"
     CDS             88..>340
                     /locus_tag="ACHKAY_005986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO5704378.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRV"
BASE COUNT           89 a           92 c           88 g           71 t
ORIGIN      
        1 cagcctaaaa gactgcttac gtccctgact atgttgtaag cctctttatc ctggtcgacg
       61 ctgaatgagc ctggtaatat acccggtatg agcagctcac ttcctgacga tatcaatgca
      121 ctgaaacgtc tccttgccga acaggaggcg ctgaaccgtg ccctgctgga aaagctgaac
      181 gagcgtgaac gcgaaataga ccatctgcag gcacagctgg ataagctgcg ccggatgaac
      241 ttcggcagcc gctccgaaaa agtctcccgt cgtatcgcac agatggaagc tgacctgaag
      301 gcacttcaga aagaaagtga tacccttacc ggtcgggttg
//
LOCUS       DAZFKM010000643          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZFKM010000643 DAZFKM010000000
VERSION     DAZFKM010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..335
                     /locus_tag="ACHKAY_005987"
     CDS             39..335
                     /locus_tag="ACHKAY_005987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO5704379.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQLVADNAKTRVPMTIGALWTAYRDVGVY"
BASE COUNT           88 a           81 c           99 g           71 t
ORIGIN      
        1 acacgccttg aaattctttg tcgtgagggc gcggaaaaat gacagaagaa cttatcaccc
       61 tggaagaagt gaaactccat tgccgcatcg atggcgacga ggaagaccag ttaatcagcg
      121 gatacattgc cgcatcgctt gaggcgtgcc agatacatat aggcaggcgc tttgatgacg
      181 ggctggagtt cacgccagcc ataaagattg gctgcatgat gtttatcgct cacctgtacg
      241 agaatcgcca gctggtagcg gataacgcaa aaacgcgcgt acccatgacg ataggcgcgc
      301 tctggacggc ttaccgtgat gtgggggtgt actgatgcc
//
LOCUS       DAZFKM010000644          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZFKM010000644 DAZFKM010000000
VERSION     DAZFKM010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..297
                     /locus_tag="ACHKAY_005988"
     CDS             <1..297
                     /locus_tag="ACHKAY_005988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704380.1"
                     /translation="ELFTDADGELITSLVVVDKPRPPVELERIEEAGNKTENHAALWQ
                     CIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKLENDGVIVIDGDDVRPL"
BASE COUNT           87 a           73 c          114 g           63 t
ORIGIN      
        1 gagcttttta ccgacgctga cggggagtta atcacgtcgc tggtggtggt ggataagccg
       61 cgccctcccg ttgaactgga gcgcatcgag gaggcaggga acaagacgga gaatcacgcc
      121 gcgctatggc agtgcatcag aacacgaaca gcacacgggg aggcgtgcac tatcgccctt
      181 ctccgcgacg atatgaaaaa gctggggtat gagatgaagc acttccggcg ctggctgtac
      241 aagctggaaa atgatggcgt aatcgttatt gacggtgatg acgtgcgccc gctgtaaaaa
      301 gtggggagta aaagtgggga gagtggggaa tttaaca
//
LOCUS       DAZFKM010000645          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZFKM010000645 DAZFKM010000000
VERSION     DAZFKM010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.651"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..238
                     /gene="yhhH"
                     /locus_tag="ACHKAY_005989"
     CDS             <1..238
                     /gene="yhhH"
                     /locus_tag="ACHKAY_005989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO5704381.1"
                     /translation="TVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSW
                     VVEGTIHSDQIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          122 a           44 c           83 g           84 t
ORIGIN      
        1 taccgttaga aatttggtta aaaataaagc tatagcattg gaattagctg agatatatgt
       61 taagaatcgt tatggacagg atgccgcaga agaagaaaaa ccatacgaaa tcactgagtt
      121 aacaacaagt tgggttgttg aaggtaccat tcactcagac caaattgctg gtggggtttt
      181 tattatagaa ataggcaaaa atgatgggag aattctgaat tttggccacg gaaaataagt
      241 atgtaaggat aatcaaacat cgtgtgcgct gatggcagag taggtggagg actccagaca
      301 gtcaaacgat agaaaaagat agcctttatg gag
//
LOCUS       DAZFKM010000646          332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZFKM010000646 DAZFKM010000000
VERSION     DAZFKM010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKAY_005990"
     CDS             complement(<1..242)
                     /locus_tag="ACHKAY_005990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO5704382.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           99 a           67 c           73 g           93 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa ca
//
LOCUS       DAZFKM010000647          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZFKM010000647 DAZFKM010000000
VERSION     DAZFKM010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>329
                     /locus_tag="ACHKAY_005991"
     CDS             <1..>329
                     /locus_tag="ACHKAY_005991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO5704383.1"
                     /translation="IIPGEQTATSILKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDP
                     ESPESFMKRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIF
                     TLPLCRE"
BASE COUNT           96 a           91 c           83 g           59 t
ORIGIN      
        1 acatcatacc gggagaacag accgccacca gcatactgaa gcagcgcaca aaaaatattg
       61 cgctaccgcc tcacacccac cagcaacaga acccaccaca ggaaaagacg gtggtcagca
      121 ttgccgttga tccggagtct ccggaatcct tcatgaaacg acctaaacgt cgccgctggg
      181 taaatgagaa atacacacgc tgggtaaaga cacagccgtg tgcgtgttgt ggtaagccag
      241 cggacgatcc tcatcatctg attggtcatg gtcagggtgg aatgggaaca aaatcccacg
      301 atattttcac gctaccgctg tgtcgggag
//
LOCUS       DAZFKM010000648          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZFKM010000648 DAZFKM010000000
VERSION     DAZFKM010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>326
                     /locus_tag="ACHKAY_005992"
     CDS             <1..>326
                     /locus_tag="ACHKAY_005992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO5704384.1"
                     /translation="WTRSIKNILTTYLFMSSGAVTARNVQTFSPTINNESRIRLPRAL
                     PVGHPYPEEHKASGFSPFMMGGLSGDILPEILTGNGPSIFFNGKHNNQHDGAFGKIID
                     FTQNGN"
BASE COUNT          109 a           55 c           70 g           92 t
ORIGIN      
        1 tggacaagga gcataaaaaa tatattgacg acatatttgt ttatgtcatc aggagcggtg
       61 acagccagaa atgttcaaac cttttctcca acaataaata atgagtcaag gattcgattg
      121 ccgagagcat tgccagtagg ccatccatat cctgaggaac ataaggcttc tggtttctcc
      181 ccttttatga tgggggggct gagtggtgat attcttccgg aaattttaac ggggaatgga
      241 ccatctatat tttttaacgg aaaacataat aaccaacatg atggagcttt tggaaaaata
      301 atagatttta cccaaaatgg aaataa
//
LOCUS       DAZFKM010000649          316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZFKM010000649 DAZFKM010000000
VERSION     DAZFKM010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..266
                     /locus_tag="ACHKAY_005993"
     CDS             <1..266
                     /locus_tag="ACHKAY_005993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO5704385.1"
                     /translation="APWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTDRFG
                     QVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
BASE COUNT           62 a           84 c           90 g           80 t
ORIGIN      
        1 ttgccccctg gttaccccgt gatgaaacaa cggcgctcac cgaggaggca ttcatcgggt
       61 gtatggccac catcaccggt tatcacgcag tggcgggttc cccctgtgaa ggtcgcttca
      121 cagaccgctt tgggcaggtt cattacctgt tgctggagcc ggaggccggg aaagaattca
      181 gacgaggaga caaagtgatt attgtctgcc gtctttccgg ttcccgctgg ctgggcgaac
      241 tgaatccctg gccggatgtt ctttaatccc gacatataca gaggttctca ctatgtttaa
      301 tcttccggga tggttg
//
LOCUS       DAZFKM010000650          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZFKM010000650 DAZFKM010000000
VERSION     DAZFKM010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>314
                     /locus_tag="ACHKAY_005994"
     CDS             <1..>314
                     /locus_tag="ACHKAY_005994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO5704386.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZFKM010000651          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZFKM010000651 DAZFKM010000000
VERSION     DAZFKM010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.657"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>311
                     /locus_tag="ACHKAY_005995"
     CDS             <1..>311
                     /locus_tag="ACHKAY_005995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704387.1"
                     /translation="EQLALAGQNTELAKLKYQTAQGELKTLTEMQKQELLRNAALIDQ
                     QKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFRQKDAD
                     L"
BASE COUNT           90 a           76 c          103 g           42 t
ORIGIN      
        1 gagcaactgg cactggcggg tcagaataca gagctggcga agctgaagta ccagactgcg
       61 cagggcgaac tgaaaaccct gacggagatg cagaagcagg aactgctgcg taacgcggcc
      121 ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac cctgaagaat
      181 gataatgtgg ctgcgcgtgc atcaaatgaa gccgaactgc tgggatacgg gcagggagaa
      241 cgagccaggg aacgcatgcg ggagttgcag cagatccgcg acagcttccg ccagaaggat
      301 gcggaccttc a
//
LOCUS       DAZFKM010000652          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZFKM010000652 DAZFKM010000000
VERSION     DAZFKM010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.658"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..115)
                     /locus_tag="ACHKAY_005996"
     CDS             complement(<1..115)
                     /locus_tag="ACHKAY_005996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704388.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGA"
     gene            complement(112..>306)
                     /locus_tag="ACHKAY_005997"
     CDS             complement(112..>306)
                     /locus_tag="ACHKAY_005997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO5704389.1"
                     /translation="GAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGL
                     PVPDATGGRLEILCRLGGEK"
BASE COUNT           62 a          112 c           75 g           57 t
ORIGIN      
        1 tcgcacccgc acgggttgcc tcacgcagca cccggttatt ttctgcacca ctcagaagct
       61 gcaaatcgcg gctgatgtcc tccagccccg aaaaatccag cagggtttcg atcatttttc
      121 acctcccagc cgacagagaa tttccagacg tccgccggtc gcatccggca cgggcagccc
      181 gacaacgttc aggatccggt cacgccaggg accactcagc acatgaagtc gtgacgctgc
      241 cgtgatttcc cgaccggact gaccgcgcac ccagatgcgg atttccgcct gcgccatttc
      301 cgcacc
//
LOCUS       DAZFKM010000653          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZFKM010000653 DAZFKM010000000
VERSION     DAZFKM010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.659"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>301
                     /locus_tag="ACHKAY_005998"
     CDS             <1..>301
                     /locus_tag="ACHKAY_005998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704390.1"
                     /translation="LADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFRNRLN
                     LSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHAPENHK"
BASE COUNT           81 a           71 c           75 g           74 t
ORIGIN      
        1 actggcagac ggaaactggt tactgaacaa tgcctggtat gaaagaaaca tggcagggtt
       61 taacgaacag ttgaaagaga acctgtcatt cacacctgat gaactgaaaa cgctcttccg
      121 gaaccgcctg aatttatcac cggaagcgaa tgacgatttt ctcgatcgtt gcctggacgg
      181 cggtgactgg tatccctttt cagaaggtcg ccgttttgta tcattccatc acgtggatga
      241 gcttcgtatc tgtgcctcct gcgggctgac agaagtacat catgccccgg aaaatcataa
      301 g
//
LOCUS       DAZFKM010000654          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZFKM010000654 DAZFKM010000000
VERSION     DAZFKM010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.660"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>301
                     /locus_tag="ACHKAY_005999"
     CDS             <1..>301
                     /locus_tag="ACHKAY_005999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO5704391.1"
                     /translation="LGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRK
                     AGNTKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLNAL"
BASE COUNT           69 a           72 c           94 g           66 t
ORIGIN      
        1 ctggggattt atctgtatac ggatgctccc gttcctgatg gtgtggcgat gacgggtacc
       61 ggtaatccgt tcctgatgtc gtacttcact cagaccactg acggcagagt gaatctgatg
      121 catcacagga aagccggaaa cacgaagctg ggggagttcg gcgattacgg taacgactgg
      181 cagacgctgg agctggtgtt caccgccggc agtgccacgg ttactccgaa actgaatgga
      241 gtggctggcc cggcattcca ggttataaaa gacagtctga cactgggact gaatgcgctg
      301 a
//
LOCUS       DAZFKM010000655          299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZFKM010000655 DAZFKM010000000
VERSION     DAZFKM010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.661"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..207
                     /locus_tag="ACHKAY_006000"
     CDS             <1..207
                     /locus_tag="ACHKAY_006000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704392.1"
                     /translation="ILQCGPGRFRNRKKLNALLNILESQFRLSVVPEGKTMYVLLPQI
                     ASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT           78 a           60 c           68 g           93 t
ORIGIN      
        1 attctccagt gtggcccggg ccggttcaga aacaggaaaa aactgaacgc attgctgaac
       61 attcttgaat ctcagttcag gctttctgtc gtgccggaag gaaaaaccat gtatgttctg
      121 cttccccaga tagccagttt aaagttgtct gatgtctctg gtatttttac ctcgggttat
      181 cactacaata agctgagagc gaagtaaccg gtgagcagtt tttctccatt atgcttcaga
      241 cttagatgat tgattattat ggggatttca tcaccgatat tcagatgtat gaatatcgg
//
LOCUS       DAZFKM010000656          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZFKM010000656 DAZFKM010000000
VERSION     DAZFKM010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.662"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..>294
                     /locus_tag="ACHKAY_006001"
     CDS             43..>294
                     /locus_tag="ACHKAY_006001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5704393.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKV"
BASE COUNT           67 a           74 c           80 g           73 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtc
//
LOCUS       DAZFKM010000657          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZFKM010000657 DAZFKM010000000
VERSION     DAZFKM010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.663"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>287
                     /locus_tag="ACHKAY_006002"
     rRNA            <1..>287
                     /locus_tag="ACHKAY_006002"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           73 a           66 c           97 g           51 t
ORIGIN      
        1 aaatgcgtag agatctggag gaataccggt ggcgaaggcg gccccctgga cgaagactga
       61 cgctcaggtg cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt
      121 aaacgatgtc gacttggagg ttgtgccctt gaggcgtggc ttccggagct aacgcgttaa
      181 gtcgaccgcc tggggagtac ggccgcaagg ttaaaactca aatgaattga cgggggcccg
      241 cacaagcggt ggagcatgtg gtttaattcg atgcaacgcg aagaacc
//
LOCUS       DAZFKM010000658          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZFKM010000658 DAZFKM010000000
VERSION     DAZFKM010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.664"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>287
                     /locus_tag="ACHKAY_006003"
     rRNA            <1..>287
                     /locus_tag="ACHKAY_006003"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           67 c           80 g           53 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgc
//
LOCUS       DAZFKM010000659          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZFKM010000659 DAZFKM010000000
VERSION     DAZFKM010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.665"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..125)
                     /locus_tag="ACHKAY_006004"
     CDS             complement(<1..125)
                     /locus_tag="ACHKAY_006004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO5704394.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYT"
BASE COUNT          103 a           65 c           45 g           72 t
ORIGIN      
        1 gtataagggg ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt
       61 gtcagctttt ccgcaagatc tgacggcgac gcagccctga caacatcata gtgtttaaat
      121 gccatggttc tttccaccat ctgaaaaata attctttaaa atacctgaca tgtaatacag
      181 aaaaaacaca aaaccatacc ttaaataaaa acctgattat caagcagata tgcatggata
      241 aactacaaga cgagatataa accaccctgt atttaaataa acaat
//
LOCUS       DAZFKM010000660          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZFKM010000660 DAZFKM010000000
VERSION     DAZFKM010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.666"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>284
                     /locus_tag="ACHKAY_006005"
     CDS             <1..>284
                     /locus_tag="ACHKAY_006005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFO5704395.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAA"
BASE COUNT           81 a           67 c          101 g           35 t
ORIGIN      
        1 aggcggataa attcgcacag cagcaacggg caaaacgggc cgccattgat gcgaaaagcc
       61 gggggctgac tgaccggcag gcagaacggg aagccacgga acagcgcctg aaggaacagt
      121 atggcgataa tccgctggcg ctgaataacg tcatgtcaga gcagaaaaag acctgggcgg
      181 ctgaagacca gcttcgcggg agctggatgg caggcctgaa gtccggctgg agtgagtggg
      241 aagagagcgc cacggacagt atgtcgcagg taaaaagtgc agcc
//
LOCUS       DAZFKM010000661          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZFKM010000661 DAZFKM010000000
VERSION     DAZFKM010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.667"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>284
                     /locus_tag="ACHKAY_006006"
     CDS             <1..>284
                     /locus_tag="ACHKAY_006006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704396.1"
                     /translation="HLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASV
                     FWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHID"
BASE COUNT           57 a           84 c           75 g           68 t
ORIGIN      
        1 catctgactt atactcaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact
       61 tatgatattg ccgagggttc ggttgtcagt ctggcggccg gagggatcag ttttgccctg
      121 acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct
      181 gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactc
      241 caccggctca gtgttgttca gggtatgctg aagcacattg attt
//
LOCUS       DAZFKM010000662          277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZFKM010000662 DAZFKM010000000
VERSION     DAZFKM010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.668"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>277
                     /locus_tag="ACHKAY_006007"
     CDS             <1..>277
                     /locus_tag="ACHKAY_006007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704397.1"
                     /translation="LHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAG
                     ILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRT"
BASE COUNT           61 a           71 c           88 g           57 t
ORIGIN      
        1 gtctgcatga tgtgcaggac atgagccgcc ttaaccatcc gaaagcggac ctgtcaaagg
       61 ggcagtacgg aaccgtgggg caagggctgc atatcgccaa aaaattgctg ccgtttatac
      121 cggcgaatgc gggcattctg ctggttccgt gctgtcgtgg tggttcagcg ttcaccaccg
      181 gagccgatgg cacatacagt gacgcgagtg gtgcctcgga gaattcaacc cgctggggtg
      241 tggacaagcc gctgtataag gaccttatcg gtcgaac
//
LOCUS       DAZFKM010000663          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZFKM010000663 DAZFKM010000000
VERSION     DAZFKM010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.669"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..95
                     /locus_tag="ACHKAY_006008"
     tRNA            19..95
                     /locus_tag="ACHKAY_006008"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHKAY_006009"
     tRNA            138..213
                     /locus_tag="ACHKAY_006009"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           71 a           62 c           73 g           67 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aag
//
LOCUS       DAZFKM010000664          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZFKM010000664 DAZFKM010000000
VERSION     DAZFKM010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.670"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           71 a           58 c           76 g           63 t
ORIGIN      
        1 gaccttgcgt gggcaaccat gcttgctgtc attaacgaac cgattggcgg cgaaggagaa
       61 aacgagcgtt tcacggttat ggagttctga tgagcagaaa aaataaaaaa gtgcgcatga
      121 gttcacgcat tgatctcgct gatgcgctca ggaaagaatc atcgctcagt gcattcacat
      181 ttgatggtcc ttatcgcctg accgggcatg acctgctgga caatatgtac tgtgctgata
      241 acgggcggtg gtatgaaacc ccggtgga
//
LOCUS       DAZFKM010000665          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZFKM010000665 DAZFKM010000000
VERSION     DAZFKM010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.671"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..197
                     /locus_tag="ACHKAY_006010"
     tRNA            122..197
                     /locus_tag="ACHKAY_006010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT           70 a           65 c           67 g           65 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagat
//
LOCUS       DAZFKM010000666          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZFKM010000666 DAZFKM010000000
VERSION     DAZFKM010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.672"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           44 a           74 c           81 g           65 t
ORIGIN      
        1 cagaatgcac ccgctgaaat acgggtatca ccatcatcgg tatcggtgat gtcgtcctct
       61 gcgggtccgg ggttggttct ggcaaccata ctcatggggt tcactcctga aaaaatcggg
      121 cggtgggtgc gcggtgtaaa cggtcacgga gtcaaaccgg aacaccgcgc acgccgcccg
      181 ctgacggggt cagtcgttaa ccgcgcttcg ccttctgcgt cgcggtggtt tttcgtgttg
      241 caggcttccg cgttgtcttt ttac
//
LOCUS       DAZFKM010000667          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZFKM010000667 DAZFKM010000000
VERSION     DAZFKM010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.673"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            9..>264
                     /locus_tag="ACHKAY_006011"
     CDS             9..>264
                     /locus_tag="ACHKAY_006011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704398.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVT"
BASE COUNT           71 a           31 c           65 g           97 t
ORIGIN      
        1 gattctatat gggaaatggt aacttttggc agttgttctt gggtaaagaa ccttacagca
       61 aaaaatctga ttttgttaag gcatggttat tgtggtgggc tattaccatc cttgttctat
      121 ttattttaag cctgactacg acattaaatt taagaagtat aggtataggg ttaagttttg
      181 ggttttgtca aatccttggt agatatacga tgtctctagg atggagtaag tactggggag
      241 ttttgggggt atttcctgta acaa
//
LOCUS       DAZFKM010000668          264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZFKM010000668 DAZFKM010000000
VERSION     DAZFKM010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.674"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>264
                     /locus_tag="ACHKAY_006012"
     CDS             <1..>264
                     /locus_tag="ACHKAY_006012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO5704399.1"
                     /translation="AEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLSDSDPSKS
                     DASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPS"
BASE COUNT           77 a           64 c           62 g           61 t
ORIGIN      
        1 gcagaaggct ggttaacgcg taaggcgcgt cgccagggta accgcaatgc gtcgaatgtt
       61 tatcagctta acgttgcgaa gcttcaggca gcggcatttt ctcaactgtc agattctgac
      121 ccgtcaaaat ctgacgcatc aaaatctgac ccgtcaaaat ttgatgcgtc gaaatctggc
      181 aaaaaagcgg gttttcaccc gtcagaatct ggcggggatc cgtcagtaaa atcaaaacat
      241 gatccgtcag ataaaaaacc ttct
//
LOCUS       DAZFKM010000669          262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZFKM010000669 DAZFKM010000000
VERSION     DAZFKM010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.675"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..71)
                     /locus_tag="ACHKAY_006013"
     CDS             complement(<1..71)
                     /locus_tag="ACHKAY_006013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704400.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..>262)
                     /locus_tag="ACHKAY_006014"
     CDS             complement(68..>262)
                     /locus_tag="ACHKAY_006014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO5704401.1"
                     /translation="GAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGL
                     PVPDATGGRLEILCRLGGEK"
BASE COUNT           54 a           97 c           65 g           46 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcccct cccagccgac agagaatttc cagacgcccg ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccatggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccggc cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca cc
//
LOCUS       DAZFKM010000670          259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZFKM010000670 DAZFKM010000000
VERSION     DAZFKM010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.676"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..>259
                     /locus_tag="ACHKAY_006015"
     CDS             61..>259
                     /locus_tag="ACHKAY_006015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5704402.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTE"
BASE COUNT           82 a           62 c           57 g           58 t
ORIGIN      
        1 tctggcagat cctgaaattc ctgagagaat agtggacacc aaatatggtg gacgctatcc
       61 atgaaatcat taaccgcagt gcgtaaaaaa agccctaatt atcccgttga gttcaaaatc
      121 aaaatggttg aactctcgca tcgaccagag atctccgtag cgcaactcgc tcgtgagcat
      181 gggatcaacg ataatttgct gttcaagtgg cgccagtact ggcgcgaagg aaaactacgt
      241 cctccttcaa caacagaaa
//
LOCUS       DAZFKM010000671          255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZFKM010000671 DAZFKM010000000
VERSION     DAZFKM010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.677"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..170)
                     /locus_tag="ACHKAY_006016"
     CDS             complement(<1..170)
                     /locus_tag="ACHKAY_006016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO5704403.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSP"
BASE COUNT           77 a           56 c           67 g           55 t
ORIGIN      
        1 atcggcgaaa cgtacagcgc catgtttacc agtcccggaa tttgtgagag atagactttc
       61 tccgtggtga agggatagct ctcacggaaa aagagacgca gaaacagcgg atcaaactta
      121 aatttctgct catttgccgc cagcagctgg gcggttgtgt acatcgacat aaaaaaatcc
      181 cgtaaaaaaa gccgcacagg cggcctttag tgatgaaggg taaggttaaa cgatgctgat
      241 tgccgttccg gcaaa
//
LOCUS       DAZFKM010000672          252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZFKM010000672 DAZFKM010000000
VERSION     DAZFKM010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.678"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>252
                     /locus_tag="ACHKAY_006017"
     rRNA            <1..>252
                     /locus_tag="ACHKAY_006017"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           61 a           50 c           90 g           51 t
ORIGIN      
        1 ctggaggtat cagaagtgcg aatgctgaca taagtaacga taaagcgggt gaaaagcccg
       61 ctcgccggaa gaccaagggt tcctgtccaa cgttaatcgg ggcagggtga gtcgacccct
      121 aaggcgaggc cgaaaggcgt agtcgatggg aaacaggtta atattcctgt acttggtgtt
      181 actgcgaagg ggggacggag aaggctatgt tggccgggcg acggttgtcc cggtttaagc
      241 gtgtaggctg gt
//
LOCUS       DAZFKM010000673          248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZFKM010000673 DAZFKM010000000
VERSION     DAZFKM010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.679"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>248
                     /locus_tag="ACHKAY_006018"
     CDS             <1..>248
                     /locus_tag="ACHKAY_006018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="IrgA-homologue adhesin (Iha); TonB-dependent; acts
                     as a specific receptor of enterobactin; fur-regulated;
                     urovirulence factor; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO5704404.1"
                     /translation="DTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYD
                     NRDGQLGSLTGGYDRTLRYERNKISAGYDHTFTFGTWKS"
BASE COUNT           63 a           57 c           70 g           58 t
ORIGIN      
        1 gatacagcag ccacgcgtat tccttatccc acggagtcac agaattataa tcttggtgca
       61 cgtcttgact ggaaggcgtc ggagcaggat gtgctctggt ttgatatgga taccacccgg
      121 cagcgttatg ataaccggga tgggcaactg gggagtctga cggggggata tgaccggacc
      181 ctgcgctatg agcgaaacaa aatttcagct ggctatgatc atactttcac cttcggaaca
      241 tggaaatc
//
LOCUS       DAZFKM010000674          248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZFKM010000674 DAZFKM010000000
VERSION     DAZFKM010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.680"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..90
                     /locus_tag="ACHKAY_006019"
     CDS             <1..90
                     /locus_tag="ACHKAY_006019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704405.1"
                     /translation="GHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT           57 a           64 c           58 g           69 t
ORIGIN      
        1 ggccatcgtg tcagtctgcc gttatcggtt ttcaatcccc aggactacca tccactgctg
       61 attaccgttt ccggaaaaaa cgttaactga tccctgaagt cagtttcatg cttcagcctc
      121 tgtcacaacg cgtggcagag gctttttatt tatcaggaga aaacagatat gccgggttac
      181 acagagtatg tcctggctga aggatctttc tcatacgggc aggcggttgc tgtcataacc
      241 gcttaccg
//
LOCUS       DAZFKM010000675          248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZFKM010000675 DAZFKM010000000
VERSION     DAZFKM010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.681"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            46..>248
                     /locus_tag="ACHKAY_006020"
     CDS             46..>248
                     /locus_tag="ACHKAY_006020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO5704406.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKA"
BASE COUNT           72 a           53 c           73 g           50 t
ORIGIN      
        1 tgcaggcggc gcagtgcgtc gcctctgact gaaaggagtt tcctgatgaa agagacgaaa
       61 aacatcgata ccgaaaacac ggtcgttgct gacactgtga aagaaaccag tgagcgtggc
      121 gtaaaactta cccaaccaat tgagcgaggc ggcgaaaaaa tcacgtatgt ggagatcacc
      181 ggagctattg agcaggctgg atctctgcga gatttgtcgc tgtctgatgt gctgaatctg
      241 aaagcgga
//
LOCUS       DAZFKM010000676          244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZFKM010000676 DAZFKM010000000
VERSION     DAZFKM010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.682"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>244
                     /locus_tag="ACHKAY_006021"
     CDS             <1..>244
                     /locus_tag="ACHKAY_006021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO5704407.1"
                     /translation="ALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESD
                     RITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAV"
BASE COUNT           57 a           68 c           77 g           42 t
ORIGIN      
        1 gcgctggacc ggttggtaca gcagggcggc tttgacgggc ggacgcaacg ttatcattat
       61 gacctgaccg gaaaactcac gcagagtgaa gatgaggggc ttgtcaccct ctggtactac
      121 gatgaatcgg accgtatcac tcaccgcacg gtgaacggcg aaccggcaga gcagtggcag
      181 tatgatgacc acggctggct gacagacatc agccacctga gcgaaggcca ccgtgtcgct
      241 gtcc
//
LOCUS       DAZFKM010000677          244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZFKM010000677 DAZFKM010000000
VERSION     DAZFKM010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.683"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           84 a           64 c           35 g           61 t
ORIGIN      
        1 tgacatctcc cccacaatac actgaaaaat actcacagca gtaaacacag acacacctga
       61 taatcatgca gccgataaat attttcccct ttacggagaa atcagcctgc tgtttaacaa
      121 taacggcgta ttttcatgga aggagatcac ccagtaacgg ataagtcctg atctccttca
      181 tgttaatacg caacaatccc atcgattcaa aacaatttca gacagatatt atctccgtga
      241 atcc
//
LOCUS       DAZFKM010000678          243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZFKM010000678 DAZFKM010000000
VERSION     DAZFKM010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.684"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..>243
                     /locus_tag="ACHKAY_006022"
     CDS             81..>243
                     /locus_tag="ACHKAY_006022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO5704408.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLG"
BASE COUNT           84 a           38 c           54 g           67 t
ORIGIN      
        1 tcctggagta aataaataac aattaacaag cataatattg ccattgataa aatagcatgc
       61 cataaaagga cttttcaggg atgagtaata ttgtttacct gacagtaacg ggagaacaac
      121 aaggaagcat ctccgcaggt tgtgggactt ctgagtctac aggtaatcgt tggcagagcg
      181 ggcatgagga tgaaatattt acattctcac tcttaaataa tattaataat acggggcttg
      241 gtt
//
LOCUS       DAZFKM010000679          242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZFKM010000679 DAZFKM010000000
VERSION     DAZFKM010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.685"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>242
                     /locus_tag="ACHKAY_006023"
     CDS             <1..>242
                     /locus_tag="ACHKAY_006023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704409.1"
                     /translation="TAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTR
                     VVSKVTALAQAHPALTRQLLIAGGALLAMTATVGSLS"
BASE COUNT           48 a           67 c           76 g           51 t
ORIGIN      
        1 acggcaaagg ttatggccaa caaccttgac ggcgatctga aatcactcag cagtgcatgg
       61 gaagggttgc gcatccgcat tgcagatctg attgacggtc cgctgcgttc tgtcacgcag
      121 tggctcacgc gtgtggtatc aaaggtgacg gcgctggcgc aggcccatcc cgcactgacg
      181 cgccagctac tgattgcagg cggtgcactg ctggcaatga ctgcaacggt tggctcgttg
      241 tc
//
LOCUS       DAZFKM010000680          241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZFKM010000680 DAZFKM010000000
VERSION     DAZFKM010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.686"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>241
                     /locus_tag="ACHKAY_006024"
     CDS             <1..>241
                     /locus_tag="ACHKAY_006024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704410.1"
                     /translation="VLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDDYFVNDEARAR
                     YWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTE"
BASE COUNT           68 a           51 c           76 g           46 t
ORIGIN      
        1 gtgctggata ttggtcgtcc tgataccgcg caggagatgc tgattaaggc agaggctgcg
       61 tttaagaaag cagacgacat ctggaatctg cgcaaggatg attattttgt taacgatgaa
      121 gcgcgggcgc gttactggga tgatcgtgaa aaggcccgtc ttgcgcttga agccgcccga
      181 aagaaggctg agcagcagac tcaacaggac aaaaatgcgc agcagcagag cgataccgaa
      241 g
//
LOCUS       DAZFKM010000681          240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZFKM010000681 DAZFKM010000000
VERSION     DAZFKM010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.687"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..155)
                     /locus_tag="ACHKAY_006025"
     CDS             complement(<1..155)
                     /locus_tag="ACHKAY_006025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704411.1"
                     /translation="MFACTFFFLHGAGCLPVNSVSAPESAIITYTWLLIAPPHRGIHH
                     AVVFLIN"
BASE COUNT           67 a           49 c           64 g           60 t
ORIGIN      
        1 ctgtttatta aaaatactac tgcatggtga atccccctgt gcggaggggc aatcagcaac
       61 caggtatatg tgataatcgc ggattcaggt gctgatactg aattcaccgg gaggcacccg
      121 gcaccatgca agaaaaagaa tgtgcatgca aacatgcccc tctccggagg ggctttttta
      181 tgggtaaaaa atgcccgaat gggttcgggc aatagcatga gatactgata ttgttgtgtt
//
LOCUS       DAZFKM010000682          239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZFKM010000682 DAZFKM010000000
VERSION     DAZFKM010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.688"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>239
                     /locus_tag="ACHKAY_006026"
     CDS             <1..>239
                     /locus_tag="ACHKAY_006026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5704412.1"
                     /translation="KKELTEPQISEGTWRKERRASYSREFKVRLAKQALQPGAVVARI
                     AREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPV"
BASE COUNT           71 a           55 c           68 g           45 t
ORIGIN      
        1 aagaaggaat taacagagcc tcaaatcagc gaaggcacat ggcgaaaaga acggcgagcc
       61 agctacagcc gtgaatttaa ggtccgtctg gctaagcagg cattacagcc cggtgctgtt
      121 gttgcccgga tcgccagaga gcacggtatc aatgataacc tgctgtttaa atggaaaagc
      181 cagtacgagg acggcttact gagcgatgat gacatacagg aatgcatgcc tgtcccggt
//
LOCUS       DAZFKM010000683          239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZFKM010000683 DAZFKM010000000
VERSION     DAZFKM010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.689"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>239)
                     /locus_tag="ACHKAY_006027"
     CDS             complement(<1..>239)
                     /locus_tag="ACHKAY_006027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704413.1"
                     /translation="YLINKTLSVYLGLFLLYPVGMILQSNYFFQATNNNRPLAVFVLI
                     ARGMSLCLIYFYNGPAGYLTSYYYVICVSGSYFLSG"
BASE COUNT          106 a           43 c           28 g           62 t
ORIGIN      
        1 ccagataaaa aatacgaacc agacacacaa atgacataat aataacttgt taaatagcct
       61 gctggtccat tataaaaata aataagacat aatgacatac cacgagcaat tagtacaaaa
      121 acagccaatg gcctattgtt attcgtagcc tgaaaaaaat aattagattg caatatcatc
      181 cctactgggt acaataaaaa caatcccaag tatacactta atgttttatt aattaaata
//
LOCUS       DAZFKM010000684          239 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZFKM010000684 DAZFKM010000000
VERSION     DAZFKM010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.690"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..233)
                     /gene="mobA"
                     /locus_tag="ACHKAY_006028"
     CDS             complement(<1..233)
                     /gene="mobA"
                     /locus_tag="ACHKAY_006028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO5704414.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDG"
BASE COUNT           53 a           70 c           46 g           70 t
ORIGIN      
        1 ccgtcgataa agagtttttt ctgtaacgcc cccagtctca taacttcttt cagtacccga
       61 tcatcagtca gtgagttaac tttcttaccg agagctgccg cccgtaaaaa accggaaacg
      121 gtcttgccgc attcagcagc cctttttctg atttcctggt cttcttctgc cgtcagacga
      181 acagggcgac tgatagcccg cctgcgcgta ttacttccac ttcgttttgt cataaaaca
//
LOCUS       DAZFKM010000685          237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZFKM010000685 DAZFKM010000000
VERSION     DAZFKM010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.691"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>237)
                     /locus_tag="ACHKAY_006029"
     CDS             complement(<1..>237)
                     /locus_tag="ACHKAY_006029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO5704415.1"
                     /translation="AERPDQLWVADFTYVSTWQGFVCVAFIIDVFAGCIVGWRVSSSM
                     ETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA"
BASE COUNT           64 a           75 c           56 g           42 t
ORIGIN      
        1 ggccagtgat acatactgag aacctttatc actgtgatgg actgtgccgg acggccgacg
       61 ggcccacaac gcctgctcca gtgcatccag cacgaatgtc gtttccatag acgatgagac
      121 tcgccacccc acgatacatc cggcaaacac atcaatgatg aacgccacac agacgaagcc
      181 ctgccatgtg ctgacgtaag taaaatcagc cacccacagc tggtcaggac gttctgc
//
LOCUS       DAZFKM010000686          231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZFKM010000686 DAZFKM010000000
VERSION     DAZFKM010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.692"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>231)
                     /locus_tag="ACHKAY_006030"
     CDS             complement(<1..>231)
                     /locus_tag="ACHKAY_006030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO5704416.1"
                     /translation="GVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLA
                     LAPWYGKKHRDNTLTMKRFSNGRGFWCLGGKA"
BASE COUNT           56 a           66 c           58 g           51 t
ORIGIN      
        1 ccgctttacc gcccaggcac cagaagccac gaccattgga aaaacgcttc atagtgagcg
       61 tgttatcccg gtgctttttg ccataccacg gagccagcgc cagcagcgac ggaatatcgc
      121 ggatggtcgg ctcaacgtgg gttttcataa agttctcggc atcaccatcc gtcggcaacc
      181 agataagtgt gttgcgctgc ttatgctcta tgaagtaggc ataaacaccc a
//
LOCUS       DAZFKM010000687          231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZFKM010000687 DAZFKM010000000
VERSION     DAZFKM010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.693"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..>231
                     /locus_tag="ACHKAY_006031"
     CDS             44..>231
                     /locus_tag="ACHKAY_006031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO5704417.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAEL"
BASE COUNT           68 a           57 c           54 g           52 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaaccca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac t
//
LOCUS       DAZFKM010000688          227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZFKM010000688 DAZFKM010000000
VERSION     DAZFKM010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.694"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>227
                     /locus_tag="ACHKAY_006032"
     CDS             <1..>227
                     /locus_tag="ACHKAY_006032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO5704418.1"
                     /translation="KRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAK
                     LELVRMNIIKQQGGMFGPNKNISEWCIPQNEG"
BASE COUNT           80 a           47 c           50 g           50 t
ORIGIN      
        1 aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca
       61 atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc
      121 aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt
      181 ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgaggg
//
LOCUS       DAZFKM010000689          225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZFKM010000689 DAZFKM010000000
VERSION     DAZFKM010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.695"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>225)
                     /locus_tag="ACHKAY_006033"
     CDS             complement(<1..>225)
                     /locus_tag="ACHKAY_006033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO5704419.1"
                     /translation="DIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNEKG
                     EPVTWQGRKYEAYPIDGSGFEMNGRGSSARP"
BASE COUNT           44 a           80 c           47 g           54 t
ORIGIN      
        1 cgggcgggca ctgctgcccc ggccgttcat ctcaaagccg ctgccgtcaa tcgggtatgc
       61 ctcatactta cgcccctgcc aggtgaccgg ctcccctttt tcattcagct cattacagaa
      121 aaaataccgc tcaccaccct gtaccgtcag gtcgatttcc cagagtacca cccgcggtga
      181 ctgctctgac ttaaccgact cgttcagact ttcttcgcga atatc
//
LOCUS       DAZFKM010000690          222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZFKM010000690 DAZFKM010000000
VERSION     DAZFKM010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.696"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>222
                     /locus_tag="ACHKAY_006034"
     CDS             <1..>222
                     /locus_tag="ACHKAY_006034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704420.1"
                     /translation="ISADIILTRTPHITEFSSDSITAQIKKNGINIQTGRMVSQKHLI
                     ITHDQEGKHLTTWYQQISLRVHSYKKHTEE"
BASE COUNT           88 a           49 c           36 g           49 t
ORIGIN      
        1 atctcagcag atattatcct gaccaggaca cctcacatta ctgaattctc ttccgacagt
       61 atcactgcac aaataaaaaa gaacgggata aatattcaga ccggaagaat ggtgtcacaa
      121 aagcatttaa tcattacgca tgaccaggaa gggaaacact taaccacatg gtatcaacag
      181 atatcattac gtgtgcactc ttataaaaaa cacacagaag aa
//
LOCUS       DAZFKM010000691          222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZFKM010000691 DAZFKM010000000
VERSION     DAZFKM010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.697"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>222
                     /locus_tag="ACHKAY_006035"
     CDS             <1..>222
                     /locus_tag="ACHKAY_006035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO5704421.1"
                     /translation="EQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINHLT
                     SVKTVTEGLAQATEQLAVEQSRLAQMQEK"
BASE COUNT           63 a           53 c           68 g           38 t
ORIGIN      
        1 cagagcagaa ccggctgatt gatgaacagg ccagtcgggt gaaatccctg caggaaaaaa
       61 tcgcaggata tcagtatgtt ctggcgaacc cgggctggac gaccggtgac ggattcatga
      121 taaaccatct gacctcggtg aagaccgtaa cggaagggct tgctcaggca acagagcagc
      181 ttgccgttga gcagtcccgt ctggcacaga tgcaggaaaa ag
//
LOCUS       DAZFKM010000692          217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZFKM010000692 DAZFKM010000000
VERSION     DAZFKM010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.698"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>217
                     /locus_tag="ACHKAY_006036"
     CDS             <1..>217
                     /locus_tag="ACHKAY_006036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704422.1"
                     /translation="TLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKMFASGGQ
                     GHGFAAERGNHIVDRVRLKNARILGDN"
BASE COUNT           65 a           46 c           56 g           50 t
ORIGIN      
        1 aaacactgtg tcaggaaatt tatgaacgcc cttacaacag ctttatttcc gatgcaacgg
       61 cgaatggtct gattctcatg aaactgccgg aaacctggag tacaaatgag aaaatgtttg
      121 cttccggagg gcagggacat gggtttgccg ctgaacgggg aaaccatatt gtcgacagag
      181 tccgtctgaa aaacgcacgg atcctcggtg ataataa
//
LOCUS       DAZFKM010000693          217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZFKM010000693 DAZFKM010000000
VERSION     DAZFKM010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.699"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>217
                     /locus_tag="ACHKAY_006037"
     CDS             <1..>217
                     /locus_tag="ACHKAY_006037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704423.1"
                     /translation="RVLLEPEPERYSSFASGAVPAASQPLADDPAVRAVFRNEAVIRR
                     AGGVECLESWLLREKGCQWPHSDWHSE"
BASE COUNT           37 a           58 c           71 g           51 t
ORIGIN      
        1 ggcgtgtcct gctggagcct gagccggaac gttattcatc tttcgccagt ggtgccgttc
       61 cggcggcatc acaaccgctg gcggatgatc ctgccgttcg ggccgtgttc cgcaatgagg
      121 cagtgatccg tcgtgctggt ggcgtggaat gtcttgaaag ctggttactt cgtgaaaaag
      181 gctgccagtg gcctcattcc gactggcaca gcgagaa
//
LOCUS       DAZFKM010000694          216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZFKM010000694 DAZFKM010000000
VERSION     DAZFKM010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.700"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>216)
                     /locus_tag="ACHKAY_006038"
     CDS             complement(<1..>216)
                     /locus_tag="ACHKAY_006038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_100495227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704424.1"
                     /translation="ASPVATSLYATAEKIIKCIQSIATPLNQYYFTRLIKQHELKLEP
                     YKVGEYKSLLYASTNIQLKFMVFIVLS"
BASE COUNT           64 a           39 c           40 g           73 t
ORIGIN      
        1 aactcaggac aatgaaaacc atgaacttta gctgaatatt tgtgcttgca tatagcaggc
       61 ttttatattc tccaactttg tatggttcta atttcaattc atgttgcttt atcaacctcg
      121 tgaaatagta ttgatttaac ggggttgcta tagactgaat acatttaata attttctctg
      181 ccgtcgcgta cagagacgtt gcaacaggcg aagcaa
//
LOCUS       DAZFKM010000695          216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZFKM010000695 DAZFKM010000000
VERSION     DAZFKM010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.701"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>216
                     /locus_tag="ACHKAY_006039"
     CDS             <1..>216
                     /locus_tag="ACHKAY_006039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFO5704425.1"
                     /translation="FFYTTNDLKSLDNVEIGGIPAKKYLEHGLFSEYRQDGTIPYVVS
                     TLNISGPDMIMRQMKKYYKSLGRIGEVH"
BASE COUNT           81 a           34 c           38 g           63 t
ORIGIN      
        1 ttcttttata ccacaaatga tctaaaatct ttagataacg tggaaatagg aggaatacct
       61 gcaaaaaaat atttagaaca cggtttgttt tctgaatatc gacaggacgg tacaattcct
      121 tatgtagtaa gtactctaaa tatatctggc ccagacatga ttatgcgaca aatgaaaaaa
      181 tactataaat ctctagggcg cataggtgaa gtacat
//
LOCUS       DAZFKM010000696          215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZFKM010000696 DAZFKM010000000
VERSION     DAZFKM010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.702"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>215)
                     /locus_tag="ACHKAY_006040"
     CDS             complement(<1..>215)
                     /locus_tag="ACHKAY_006040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO5704426.1"
                     /translation="EGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVS
                     TLCISQFRYSAQIRPSSVVTKDYTFKRP"
BASE COUNT           45 a           45 c           71 g           54 t
ORIGIN      
        1 gggcgtttaa aggtgtagtc tttggtcacc acggaagaag ggcggatttg tgcgctgtag
       61 cggaactggc tgatgcagag ggtgctcacc tcggtacggg tgttcgggtt ccatgggatt
      121 tcaaaagatt cgggcagatg gcggactgtg tcgcacagca ccaggctctg gtcggtactt
      181 ttgtaagcat gctcctcata aaagaagatg ccttc
//
LOCUS       DAZFKM010000697          213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZFKM010000697 DAZFKM010000000
VERSION     DAZFKM010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.703"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>213
                     /locus_tag="ACHKAY_006041"
     CDS             <1..>213
                     /locus_tag="ACHKAY_006041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO5704427.1"
                     /translation="GYAQVHPGGVPALSGTGARAGDLKGINVKYRYEFTDHLGGIVAL
                     SYASVKKSDTMKTGENTFHYESLRGR"
BASE COUNT           52 a           44 c           67 g           50 t
ORIGIN      
        1 gggatatgct caggttcacc cgggcggcgt accggcattg tccggtaccg gtgcgcgtgc
       61 aggtgattta aaagggatta atgtgaaata ccgttatgag ttcacggatc acctgggcgg
      121 cattgtcgcg ctgagttatg catcggtgaa gaaaagtgac acgatgaaga cgggtgaaaa
      181 taccttccat tatgaaagcc tgcgcggtcg tta
//
LOCUS       DAZFKM010000698          213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZFKM010000698 DAZFKM010000000
VERSION     DAZFKM010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.704"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>213
                     /locus_tag="ACHKAY_006042"
     CDS             <1..>213
                     /locus_tag="ACHKAY_006042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO5704428.1"
                     /translation="FELGENDKNQMLYSMKVHPPLWRAGLRQNFRIFQNEDIKSILGT
                     MLQENGVTEWSPLFSEPHPSREFCVQ"
BASE COUNT           51 a           57 c           62 g           43 t
ORIGIN      
        1 gtttgaactc ggggagaacg acaaaaacca gatgctgtac agcatgaagg tgcacccgcc
       61 gctgtggcgt gccggtctgc gccagaactt ccgtatcttc cagaacgagg acatcaaaag
      121 catcctcggc acgatgttgc aggaaaacgg ggtgaccgaa tggagtccgc tgttcagcga
      181 gccgcatcct tcccgtgagt tttgtgtcca gta
//
LOCUS       DAZFKM010000699          213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZFKM010000699 DAZFKM010000000
VERSION     DAZFKM010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.denovo.705"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>213
                     /locus_tag="ACHKAY_006043"
     CDS             <1..>213
                     /locus_tag="ACHKAY_006043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO5704429.1"
                     /translation="IRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALDGL
                     FWFGSQRIAADVLRLRKAGMPVVTTTV"
BASE COUNT           44 a           47 c           73 g           49 t
ORIGIN      
        1 tattcgtcgc gtggttggga atgaaaacac atcattgcca tatcagcagg ttgcccggga
       61 agtgaaagcg ccccgtgaac ggctgaagta tgcactggag gcgggtttac tggtgactgc
      121 actggacggc ctgttctggt ttggcagtca gcgtattgcg gctgatgttc tgaggctgag
      181 aaaggccgga atgccggtgg tgaccacgac cgt
//
LOCUS       DAZFKM010000700        17907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.guided.098, whole genome shotgun sequence.
ACCESSION   DAZFKM010000700 DAZFKM010000000
VERSION     DAZFKM010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.guided.098"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..238
                     /locus_tag="ACHKAY_006044"
     CDS             <1..238
                     /locus_tag="ACHKAY_006044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704430.1"
                     /translation="LGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQ
                     KSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKAY_006045"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKAY_006045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO5704431.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKAY_006046"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKAY_006046"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO5704432.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHKAY_006047"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHKAY_006047"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO5704433.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKAY_006048"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKAY_006048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO5704434.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6382..6696)
                     /locus_tag="ACHKAY_006049"
     CDS             complement(6382..6696)
                     /locus_tag="ACHKAY_006049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO5704435.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6693..7007)
                     /gene="yjeN"
                     /locus_tag="ACHKAY_006050"
     CDS             complement(6693..7007)
                     /gene="yjeN"
                     /locus_tag="ACHKAY_006050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO5704436.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="ACHKAY_006051"
     CDS             complement(7059..8561)
                     /gene="yjeM"
                     /locus_tag="ACHKAY_006051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO5704437.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="ACHKAY_006052"
     CDS             complement(8780..9757)
                     /gene="epmA"
                     /locus_tag="ACHKAY_006052"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO5704438.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10082..11890
                     /gene="frdA"
                     /locus_tag="ACHKAY_006053"
     CDS             10082..11890
                     /gene="frdA"
                     /locus_tag="ACHKAY_006053"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO5704439.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11883..12617
                     /gene="frdB"
                     /locus_tag="ACHKAY_006054"
     CDS             11883..12617
                     /gene="frdB"
                     /locus_tag="ACHKAY_006054"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO5704440.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12628..13023
                     /gene="frdC"
                     /locus_tag="ACHKAY_006055"
     CDS             12628..13023
                     /gene="frdC"
                     /locus_tag="ACHKAY_006055"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO5704441.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13034..13393
                     /gene="frdD"
                     /locus_tag="ACHKAY_006056"
     CDS             13034..13393
                     /gene="frdD"
                     /locus_tag="ACHKAY_006056"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO5704442.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            13456..14589
                     /locus_tag="ACHKAY_006057"
     CDS             13456..14589
                     /locus_tag="ACHKAY_006057"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO5704443.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14678..15211
                     /gene="blc"
                     /locus_tag="ACHKAY_006058"
     CDS             14678..15211
                     /gene="blc"
                     /locus_tag="ACHKAY_006058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO5704444.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15208..15525)
                     /gene="sugE"
                     /locus_tag="ACHKAY_006059"
     CDS             complement(15208..15525)
                     /gene="sugE"
                     /locus_tag="ACHKAY_006059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO5704445.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15707..15853)
                     /gene="ecnB"
                     /locus_tag="ACHKAY_006060"
     CDS             complement(15707..15853)
                     /gene="ecnB"
                     /locus_tag="ACHKAY_006060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO5704446.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15964..16089)
                     /gene="ecnA"
                     /locus_tag="ACHKAY_006061"
     CDS             complement(15964..16089)
                     /gene="ecnA"
                     /locus_tag="ACHKAY_006061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO5704447.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16141..16707)
                     /gene="efp"
                     /locus_tag="ACHKAY_006062"
     CDS             complement(16141..16707)
                     /gene="efp"
                     /locus_tag="ACHKAY_006062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO5704448.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16749..17777
                     /gene="epmB"
                     /locus_tag="ACHKAY_006063"
     CDS             16749..17777
                     /gene="epmB"
                     /locus_tag="ACHKAY_006063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO5704449.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4448 a         4742 c         4628 g         4089 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggccgcttta aactcggctc taccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca
     3781 gcgccgattt gccgaaagac atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgc caggccacca
     3901 gccagacgtt acaggttcgg caggcgctga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaattca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggaact gaccaagctg aaagtctcca
     4261 acgggcaact tgaggatgcg ctgaaagagg tgaatgaagc gacgcatcgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccact ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtacctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccactgg ttgccattct ggcgtcagcg gtgggttatc
     5101 tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgtcgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggag attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gataccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaaacaaa
     6301 cggggagaac gctgacgtct gcgggcaaag gtcgtcaggc gggaagtttg taagtttgtg
     6361 tgccggatga accaatccgg ctcacaaaca aaactgatag cgcagaaaaa actgccacag
     6421 ccagtagccg aaaatagcca gcgcggtggc gtacataaac cagccgcgtt tttttcgcca
     6481 caaagcgaac cagagaaaag ggaaaaagag cggtaacgtc attatcacac caacatcacg
     6541 ggtatcgtct tcttcgagat acatcaacac atcgcagacg ttgttaatcc cgcgtccatc
     6601 aatcatccac tctttgtcca gcgcggaagt gatggttatc cagaggaacg ccacaataaa
     6661 ccagatgcag cataagacaa acatgcgcgc actcataagg taaccggtcg cttaatttcg
     6721 ctatagcgca tacgaaacaa atcgccgtat cccactaatt ccccgcgaaa aaccagacat
     6781 cggataagat aagcgcaata aatatccggc aggtttcccc accgtttacc atcgcgatct
     6841 ttgagggtgt taatggtgct acccaccacc atcgccactt tacgggtgtg gcaggcatcg
     6901 acaaaagaga gaatcgtctg ctctatttcc gctacatccc cggcagaaaa ttgcagcgcc
     6961 ctgatttcat cttcggttat tgccgggtcc cggctggtgt catccataac atttcctgtc
     7021 tttacgccct atcgtcaggt agacgatagg gcatcgggtt attccaccgc ccactgcgga
     7081 ttttttgcca ctctgctgca atagttctgg taaatcgcca tcgctaacag cgagaagaag
     7141 acagggccgc caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggttga
     7201 atgatggtga agacgttcgc aaatgtcacc accagtacga caaccactgt tgcaatcatt
     7261 gccgacatat gcgttttgaa aatcacaaat ggtctgtcga gatcctgacg tgctttaaaa
     7321 aatgggaaag ccagcgcgag gaacaggtaa ggaagcgtca tagacacgtt cgccatcagc
     7381 gtcagcttgt taaagaacgc cgatgcggta ccgccaccaa acgaaaccag caggatgaag
     7441 acagtaacca acccgcactg catccacatg gcgatagacg gcatccccat cgcattcagg
     7501 cgcgtcatcg gttccggcca caacgctttc ggcgtcccct ggatgatggc tttcagcggt
     7561 gaatagcaaa gcgtaaagaa cgcaccggta taagcgagga acatcgaaag tccggtaata
     7621 cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg acaaatgcag tgcgttaccc
     7681 agcgtcaccc cgaggctctt catcagcaca taggtaatat tgccaaggtt aacggaacca
     7741 ttacttaata cctgctgcca gtttgtgctg acgccccata aaaatattgc cagcgaataa
     7801 ccgattgaaa taacaatagc ggcaaaaaca atacctttgg caaagttctt ttctggattt
     7861 tccgttttat cgaccagacc accaaccgct tcaattccgc cataggcaaa aatagcaaat
     7921 accacaaacg atagcattgc cagaccggac tgataacctg ggttcggtga tgcaaggaaa
     7981 ttaatatcct gcgcgaaatg cccaccattt aataacaaaa tagtaatgct tactaacagc
     8041 aatactaaat tcagacacat tactgcaata ccgcccaccg cagtaatgcg ggcaatttta
     8101 ttaatcccct ttgaagcaac gacggtgacc agaatcatcc atgccactgc cagcagacca
     8161 accacctgcg taggctccag tccagcaata cgccagtgct gggtcatgtc gctaccatag
     8221 aggaatgttg agaacggtac ccaaactttc gcggaggtac tcaccatcca gatgatataa
     8281 gaggaaaacc acataaacgt accaatgaag gcaaaacgtg gtccgacact attattcatc
     8341 caggaataga taccgccttc ttctttgcga taagcagctc ccatttcagc catcattaag
     8401 gcgaatggaa taaagaataa caatgcagaa aatatataaa agggaatcgc actataaccc
     8461 attaagtaat aagccgatgg gctattggca aatccaaata cggaagtaaa gatcatcaat
     8521 atgagtccta tcagactcat cttttttatc gtgtgaggca tgaaaccatc cttcatttca
     8581 gcgcgtgtat aacacgtgcc cactgaactg cactgcgcaa cattgagcag gcagcgtaaa
     8641 aaccaataca gaattgatac ctggatgata tcaaatatcc agtcttaaat tgtggccttt
     8701 aacggaaaaa tgaaaacata tgctacagaa tggcgcttat cacgccattc ttcgctgtta
     8761 attcagtaat ttttcagaat tatgcccggt caacgctaaa ggcgatgact tcagccagtg
     8821 tctccgcgcc cagcgccaac atcaccagac gatcaacacc taatgccacg ccggaacagt
     8881 caggcatacc gactttcaag gcttcaatca gattctggtc aatggggtgc tgcggcaaac
     8941 cgcgcgccgc gcgcttacgg ttatcttgtt caaagcgttg ttgctgctca cgggcatccg
     9001 tcaattcatg gaaaccattc gccagctcaa tacctttata ataaacctca aagcgttcag
     9061 cgacccgatg atcttcggta ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt
     9121 acacaaaggt cggtttttct ttgccaatat ttggctctac gccaaaggta aacagcaatt
     9181 gtagcagcgt gtcgcggtct tcttcggtat cagcaacatt gctcaaatcc agtttcgctg
     9241 cgacttcccg cagttgcgtt ttgtcggcag agagcgggtc aatttccaga taacgcaaga
     9301 aagcttgttg ataagaaagg ctttctgctg ccgggcagtc cagcacctgt tgtaagagat
     9361 cgtccacctc gttcatcaac cggtacatat catagtgcgg tcgataccac tccagcatag
     9421 tgaactcagg gttgtgataa cgccccatct cttcattacg gaagctgcgg cacagctgga
     9481 ataccggccc acaaccggca accagcaggc gtttcatatg gtattccggg ctggtcatta
     9541 accagagatt catcccctgc gaatgcccgg ggccaacgaa acgtgtctca aacgggacca
     9601 aatgaatatc ggttaccgtc gcctggctca tacaaggcgt ctccacctcc agcactccac
     9661 gatcggcaaa gaaacgacgg atctccgcca taatcgccgc gcgttttaat aagttaggaa
     9721 tggatgcgct cggctgccag gatgccgttt cgctcatagt taaatctcca gtttttgaca
     9781 agggcacgaa gtctactcgc aacgcgacgg cgagacaaat tttacgcagg aatcaaacag
     9841 cggtgggcag tgactaaaaa aagcacgatc tgatggttta gtaattaaat taatcatctt
     9901 cagtgataat ttagccctct tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta
     9961 tccatcagac tatactgttg tacctataaa ggagcagtgg aatagcgttc gcagaccgta
    10021 actttcaggc acttaccctg aagtacgggg ctgtgggata aaaacaatct ggaggaatgt
    10081 cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc
    10141 tgcaattgct gccgcgcagg caaatccaaa tgcaaaaatc gcactaatct caaaagtata
    10201 cccgatgcgt agccataccg ttgctgcaga agggggctcc gccgctgtcg cgcaggatca
    10261 tgacagcttc gaatatcact ttcacgatac agtagcgggt ggcgactggt tgtgtgagca
    10321 ggatgtcgtg gattatttcg tccaccactg cccaaccgaa atgacccaac tggaactgtg
    10381 ggggtgccca tggagccgtc gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat
    10441 gaaaatcgag cgcacctggt tcgccgccga taagaccggc ttccatatgc tgcacacgct
    10501 gttccagacc tctctgcaat tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga
    10561 tattctggtt gatgatggtc atgttcgcgg cgtggtagca atgaacatga tggaaggcac
    10621 gctggtgcag atccgtgcta acgcggtcgt tatggctact ggcggtgcgg gtcgcgttta
    10681 tcgttacaac accaacggcg gcatcgttac cggtgacggt atgggtatgg cgctaagcca
    10741 cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac ccaaccggtc tgccaggttc
    10801 cggtatcctg atgaccgaag gctgccgcgg tgaaggcggt attctggtca acaaaaatgg
    10861 ctaccgttat ctgcaagatt acggcatggg cccggaaact ccgctgggcg agccgaaaaa
    10921 caaatatatg gaactgggtc cacgcgacaa agtttctcag gccttctggc acgaatggcg
    10981 taaaggcaac accatctcca cgccgcgtgg cgatgtggtt tatctcgacc tgcgtcacct
    11041 cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc gaactggcga aagcgtacgt
    11101 tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg accgcacact acaccatggg
    11161 cggtatcgaa accgatcaga actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga
    11221 atgttcctct gttggtctgc acggtgcaaa ccgtctgggt tctaactccc tggcggaact
    11281 ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa
    11341 tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct
    11401 ggttaaccag gatggcggcg aaaactgggc gaagatccgc gacgaaatgg gcctggcaat
    11461 ggaagaaggt tgcggtatct accgtacgcc ggaactgatg cagaaaacca tcgacaagct
    11521 ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa
    11581 caccgacctg ctctacacca ttgaactggg ccacggtctg aacgttgctg aatgtatggc
    11641 gcactccgca atggcacgta aagagtcccg cggcgcacac cagcgtctgg acgaaggttg
    11701 caccgagcgt gacgacgtca acttcctcaa acacaccctc gccttccgcg atgctgatgg
    11761 cacgactcgc ctggagtaca gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt
    11821 ttacggtggc gaagcggatg cagccgataa ggcggaagca gccaataaga aggagaaggc
    11881 gaatggctga gatgaaaaac ctgaaaattg aggtggtgcg ctataacccg gaagtcgata
    11941 ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc aactacctca ttactggatg
    12001 cgctgggcta catcaaagac aacctggcac cggacctgag ttaccgctgg tcctgccgta
    12061 tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa
    12121 ccttcctgcg tgattacacc gacggtatga aggttgaagc gttagctaac ttcccgattg
    12181 aacgcgatct ggtggtcgat atgacccact tcatcgaaag tctggaagcg atcaaaccgt
    12241 acatcatcgg caactcccgc accgcggatc agggtactaa catccagacc ccggcgcaga
    12301 tggcaaagta tcaccagttc tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc
    12361 cgcagtttgg cctgaaccca gagttcatcg gtccggctgc cattacgctg gcgcatcgtt
    12421 ataacgaaga tagccgcgac cacggtaaga aggagcgtat ggcgcagttg aacagccaga
    12481 acggcgtatg gagctgtact ttcgtgggct attgctccga agtctgcccg aaacacgtcg
    12541 atccggctgc ggccattcag cagggcaaag tagaaagttc gaaagacttt cttatcgcga
    12601 ccctgaaacc acgctaagga gtgcaacatg acgactaaac gtaaaccgta tgtacggcca
    12661 atgacgtcca cctggtggaa aaaattgccg ttttatcgct tttacatgct gcgcgaaggc
    12721 acggcggttc cggctgtgtg gttcagcatt gaactgattt tcgggctgtt tgccctgaaa
    12781 aatggcccgg aagcctgggc gggattcgtc gactttttac aaaacccggt tatcgtgatc
    12841 attaacctga tcactctggc ggcagctctg ctgcacacca aaacctggtt tgaactggca
    12901 ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa
    12961 agtctctggg cggtaactgt ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg
    13021 taaggagcct gagatgatta atccaaatcc aaagcgttct gacgaaccgg tattctgggg
    13081 cctcttcggg gccggtggta tgtggagcgc aattattgcg ccggtgatga tcctgctggt
    13141 gggtattctg ctgccactgg ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct
    13201 ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct
    13261 gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat ctgaaaattc acgtgcctgc
    13321 gggcaaatgg gttttctacg gtctggctgc tatcctgaca gttgtcacgc tgattggtat
    13381 cgttacaatc taacgtatcg ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta
    13441 tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac cgcctcttgc
    13501 tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat taccccgctt
    13561 atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg taaaccttat
    13621 tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg
    13681 tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga cgctattgct
    13741 cgaggggaaa tcaagttaag cgatcccaca acaaaatact ggcctgaact taccgctaaa
    13801 cagtggaatg ggatcacact attacatctc gcaacctaca ctgctggcgg cctgccattg
    13861 caggtgccgg atgaggtgaa atcctcaagc gacttgctgc gcttctatca aaactggcag
    13921 cctgcatggg cgccaggaac acaacgtctg tatgccaact ccagtatcgg tttgttcggc
    13981 gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac acgtgtcttc
    14041 cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga aaagaattac
    14101 gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt agatgctgaa
    14161 acttatggtg tgaagtcgac cattgaagat atggcctgct gggtacgaag caatatgaat
    14221 ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca atctcgctac
    14281 tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg gccggtaaat
    14341 cctgacagca tcattaacgg cagtggcaat aaaattgcac tggcagcaca ccccgtaaaa
    14401 gcgattacgc ccccaactcc tgcagtacgc gcatcatggg tacataaaac aggggcaacc
    14461 ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat agtgatgctg
    14521 gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctggcagat tctcaacgct
    14581 ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt tccttgctgt
    14641 catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc ctctcgttgc
    14701 cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc cgcgtggcgt
    14761 aacagtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg
    14821 ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga
    14881 tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca
    14941 gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt
    15001 ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat accgccatgc
    15061 gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc caaccatttc
    15121 tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa
    15181 gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac caataatccc
    15241 caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac cgagcagcac
    15301 aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat aagccgtccc
    15361 tactggtaac gatttcatcg cccaggcaag tagcgccata ctgacaatca tcgccgtcac
    15421 ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc caacggccca
    15481 taccacttcc agcagaccag caataactaa gataatccag gacatatcag gctccggaga
    15541 attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag ctgatggttg
    15601 aacggatatt ttgccaggtg gcaggggaat ttcaactatt accttagtga ctatcgcaga
    15661 aaaaacaaac ggcacgacat acctgtcgta ccgttattgc ttattgctat tgctgcgctt
    15721 tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca acgccacgcg
    15781 tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc gctgcaattg
    15841 tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc tatagcaaat
    15901 taataataga caacatcgcc aaaaatgacg gaaatctgat gatttttcgg aagagaagaa
    15961 aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt cgccgaaacc
    16021 gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa cgataaggcg
    16081 tttcatcatc tctgacctga ataagtgatg gtgcagcctg caggccgcac cacaaccgca
    16141 ttacttcacg cgagagacgt attcaccaga gcgggtatcc actttgatga cttcgccgat
    16201 ttgtacgaac agcggaacct taaccacagc gccagtagac agggtagccg gtttgccgcc
    16261 agtacctgcg gtatcacctt tcaggcccgg atcggtatca acgatttcca gttcaacgaa
    16321 gttcggcgga gtaacggaga tcggctgacc attccacaga gttacgatac actctgcctg
    16381 atccagcagc catttagcgt tgtcgccaat tgcttttgca tcagcagaca gctgctcgaa
    16441 agtttcgttg ttcatgaagt gccagaactc accgtcgttg tacaggtaag tcaggttcat
    16501 atcgacaaca tcagcgcctt cagcggaatc agtagatttg aaggtttttt ctacgcgagt
    16561 accggtcagc agacgacgca gtttaacgcg agcaaatgcc tggcctttac ccggttttac
    16621 gaattcactc gcttcaaccg cgtaaggttc gccgtctaac atgattttaa gaccagcacg
    16681 aaaatcgttg ctatagtacg ttgccataag gccctctgaa atttgttaat tggtagctaa
    16741 gccacaaaat ggcgcatatt gtaaccctaa ataccccatc cagagaagat tggttaacgc
    16801 aacttgccga tgttgtgacc gatcctgatg aacttctgcg tcttttgaat atagacgcgg
    16861 acgaaaaact gttggccgga cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct
    16921 catttatcga tcgcatggag aaaggcaatc cggacgatcc tcttttgcgt caggtactta
    16981 cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac cgacccgctg gaagaacagc
    17041 acagcgtagt gcctggtttg ttgcataaat accacaaccg ggcgcttttg ctggtcaaag
    17101 gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca cttcccctat gccgaaaatc
    17161 agggcaacaa gcgtaactgg cagactgctc ttgagtatgt tgctgcgcat ccggaactgg
    17221 acgagatgat tttctccggc ggcgatccgc tgatggcgaa agatcacgag ctggactggt
    17281 tgctcacaca actggaagcc atcccgcata tcaaacgtct gcggattcac agccgtctgc
    17341 cgattgtgat cccggcacgt atcaccgagg cgctggttga acgctttgcc cgttctacgc
    17401 tgcaaatctt gctggtgaat cacatcaatc atgccaatga ggtagatgaa acgttccgtc
    17461 aggcgatggc taaattgcgc cgtgtcggtg tcaccctgct taaccagagc gttctgttac
    17521 gtggtgtgaa cgataacgca caaacgctgg caaacctgag taatgcgttg ttcgatgccg
    17581 gcgtaatgcc gtattacctg catgtgctcg ataaagtaca gggcgcggcg cattttatgg
    17641 tgagtgatga cgaagcacgg cagattatgc gtgagttgct gacactggtg tcgggttatc
    17701 tggtgccgaa actggcgcga gaaatcggcg gcgaacccag caaaacgccg ctggatctcc
    17761 agctacgcca gcagtaactg attaaacagg gaagttaaac attagcctga tgcgctacgc
    17821 ttatcaggcc tacattgtgc attgaaattg aatggatttg caatgaactg taggccggat
    17881 aaggcgttta cgccgcatcc ggcaaga
//
LOCUS       DAZFKM010000701         8463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.guided.184, whole genome shotgun sequence.
ACCESSION   DAZFKM010000701 DAZFKM010000000
VERSION     DAZFKM010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.guided.184"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1140..2126
                     /gene="mobV"
                     /locus_tag="ACHKAY_006064"
     CDS             1140..2126
                     /gene="mobV"
                     /locus_tag="ACHKAY_006064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFO5704450.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHL
                     AARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWL
                     ADKYGAENVVTATIHRDELTPHLSAFVVPRTADGRLSAKEFIGNRTKMSQDQTSYAER
                     VADLGLERGIEGSRATHQRVKTHYGAIQQAGRDVPHLTPDELNPQKVKGVSLAEKVFG
                     AVETVEGVAQRLNAKIMGSVQPMAEKAAVSAQNERRAKELRETLAQQQKRLQALQEPF
                     KGLSKDQVAGLIRQAVKLRQENEQEKQERAQQIKERFKAKRERERSDRSRGR"
     gene            complement(2361..2582)
                     /locus_tag="ACHKAY_006065"
     CDS             complement(2361..2582)
                     /locus_tag="ACHKAY_006065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704451.1"
                     /translation="MAMSMYYIKTGLEYWKPKGYGYTKSREEAGVFTLKDMEELNLDG
                     CTLERAEVAFLDEDGPSPDLPHRVTLPRD"
     gene            complement(2573..2776)
                     /locus_tag="ACHKAY_006066"
     CDS             complement(2573..2776)
                     /locus_tag="ACHKAY_006066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO5704452.1"
                     /translation="MTDPLKALFGKPDYSHIVRDTTATISITAAEMAAVLEAYDRGID
                     TLDGTTRTALDSVISKLKDEVWP"
     gene            3113..3928
                     /gene="sul2"
                     /locus_tag="ACHKAY_006067"
     CDS             3113..3928
                     /gene="sul2"
                     /locus_tag="ACHKAY_006067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO5704453.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3989..4792
                     /locus_tag="ACHKAY_006068"
                     /note="aph(3'')-Ib"
     CDS             3989..4792
                     /locus_tag="ACHKAY_006068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO5704454.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4792..5628
                     /locus_tag="ACHKAY_006069"
     CDS             4792..5628
                     /locus_tag="ACHKAY_006069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO5704455.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIKDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5741..5923)
                     /locus_tag="ACHKAY_006070"
                     /pseudo
     CDS             complement(5741..5923)
                     /locus_tag="ACHKAY_006070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(5962..>8463)
                     /locus_tag="ACHKAY_006071"
     CDS             complement(5962..>8463)
                     /locus_tag="ACHKAY_006071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="HFO5704456.1"
                     /translation="RQKAASSLTLQQCHKLALIPSAEQRSQLEMLLGPTDCSRLSLLE
                     SLKKGPVTISGPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIA
                     RMSPQKRMAVLVAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDK
                     SALALASACSYLLKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMV
                     EQYGRVRRFLPHLLNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSW
                     KRLVINKEKHITRRGYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANR
                     IKVYRSLGHPTDPQEAIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLA
                     SLDEPDSLKRLSKMISDLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSI
                     SAVLMAEACNIGLEPLIRSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPL
                     AQIWGGGEVASADGMRFVTPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIP
                     GTLRDSIFVLEGLLEQETGLNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASV
                     FWRMDHDADYGVLNDIARGQSDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSE
                     RPSGLTQAIIEVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGE
                     IRKRYTDGQEDQLGTLGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCH
                     GHINMLGHYSFTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT         1848 a         2380 c         2346 g         1889 t
ORIGIN      
        1 tcactaacgt acgttttcgt tccattggac ttcagacccc ctccctgacg cgccgccatc
       61 cctttgaata tacctgtgtg aaccccttgt tctttcgcgc ctcctcgcgc tcctgagcct
      121 ccttctcgtc tgctgctgcc agctcccttt gcctgattcg tctctgtgtc tctgtcgcta
      181 cggtgacgct ttgcattgtt gacctcctaa ccggaaacag ggcgtccgct taaccggacg
      241 tttaattcct accataggaa aattacttcc caaaaccgtt tcccaagtca agcaatttgt
      301 ttcctatgat aggaagtaag cggaaacaag ctgcttccgg ttgtccggaa ccctacgttt
      361 cctatcacaa aatttaaaaa ctatataaat caatcattta gtatttttcc ccttctcctc
      421 taagggactg ccgtcccgcc ggcgcttcgc ttggcaccct gctgacggtc gctgcgctcc
      481 cttggagtta atgcctcgcc agaggcgaga gggtttgttt ccgctgcgct aggcccaaag
      541 gggctttgcc cctctggact ccccacactc gccgccgtga ggctcggggg ctggggcctg
      601 cggtcgctgc gctcccttcg ttcccaaccc cagagccaga ggctgggtcg ttccggcagc
      661 gtcaaggggc tttcgcggca tatcctcagg cgctgcgcgc cttgcggtat tccacgaccc
      721 cttgaccccg cctccactcg gaacggaatc aggtcaaggg cgggagcgga agccgcagcc
      781 ctggagcgaa gcggaaggga tcgggtgagc acccggcgaa gcgaaccctt gacgtgatgg
      841 agtgaccccc acgctcaggc aagggggcag gtagagcaga gcgaagcggc gcggccacct
      901 gcacccctgc cacgtggcga acgcggggtt tggggcagcg ccccaaggtg ttcgcaacga
      961 ccgaagggag gcgcagggcg aagcccgtag agcaaggccg caggccgctg caaccgcgta
     1021 gcggtcacgt actttacgga gtaaagtcta gtgagtttac acttcaaaga agtggcccgc
     1081 cggaggcaca ttcgtacctc gacgggctgg cagcaatctg acagtgcgga gggggacgga
     1141 tggcgtttgc gatcatgagg gcgaagaagc tcaacagcat gggcacggtg gcggctgcgt
     1201 tgcagcactg ctaccgcgac cgcgagacgc cgaacgccga tcaggagcgc acaccggaca
     1261 acgatcacct ggcggccaga tcgaccgatg aggcgatggg caagctcagg gagcggttgc
     1321 cggagaagcg gcgcaaggat gcggtgctgg cggtcgaata cgtgatgagc gcctcgccgg
     1381 agtggtggca gaccgcaagc gccgaccagc agcgggagtt cttcaagcga tcgaccgagt
     1441 ggctggccga caagtacggc gctgagaacg tggtcacggc cacgatccac cgggatgagc
     1501 tgaccccgca cctgtcggcg ttcgtggtgc ctcggacggc tgacgggcgc ctgagtgcca
     1561 aggagttcat tggcaaccgg acgaagatga gccaggacca gaccagctac gccgaacggg
     1621 tggccgatct ggggcttgaa cggggcatcg agggcagcag ggccacacac cagcgagtta
     1681 aaacccacta tggggcgatc cagcaggcag gccgagacgt gccacacctg acgcctgacg
     1741 agctgaaccc ccagaaggtc aagggggtgt cactggccga gaaggtgttc ggagccgtgg
     1801 agacggtcga aggggtggcg caaaggttga acgccaaaat aatggggtct gtgcagccga
     1861 tggccgagaa agccgctgta agcgcccaga acgagcgaag agccaaggag ctaagggaaa
     1921 ccctagccca gcagcaaaaa cgcttacagg cccttcagga gccgtttaag gggctatcca
     1981 aggatcaggt agcgggactg atccggcagg cggtgaaatt gcggcaggag aacgagcagg
     2041 agaagcagga gcgcgcccag cagatcaaag agcgattcaa ggccaagcgg gaacgggagc
     2101 gcagcgaccg cagcaggggc cgttaaccct tttcaaccaa ctttaaccaa ccttgaacaa
     2161 ccttaaacca ttgtttaacc aacattaaac cactttcaac caaccctaaa ccactttcaa
     2221 ccaaccctaa gcaaccgggg tgtaagggct ggcagtaatc gcgcggattt acaggcacgt
     2281 cggatatgtt tacaaaaact ccgtttttga cacatatccc gtgccaaagg gtgccccttt
     2341 gaaaccctcc agcgccccgc tcaatcgcgg ggcagggtga cgcggtgggg taggtcggga
     2401 ctggggccgt cctcgtccag gaacgccact tcggcgcgtt ccagggtgca gccatcgagg
     2461 ttcaattctt ccatgtcctt gagggtgaaa acccccgcct cctcgcggct cttggtgtac
     2521 ccgtagccct tgggtttcca gtactccaga cctgtcttga tgtaatacat gctcatggcc
     2581 atacctcgtc cttgagtttc gatataacgg aatccagcgc cgttctggtg gtgccgtcca
     2641 gcgtgtctat acccctgtcg taagcctcca aaacagcggc catttcagcg gctgtgatgc
     2701 tgatagtggc cgtggtgtcc cttacgatat gggagtaatc aggcttgccg aaaagggctt
     2761 tgagtggatc tgtcatggct gatctccaaa catatcaagc gtgtctgtat cccactctat
     2821 tgtaaacaag acatttttat cttttatatt caatggctta ttttcctgct aattggtaat
     2881 accatgaaaa ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga
     2941 gcgctgccgc acagctccat aggccgcttt cctggctttg cttccagatg tatgctcttc
     3001 tgctcctgca gctaatggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     3061 ccgagcatcc gtatgatact catgctcgat tattattatt atagaagccc ccatgaataa
     3121 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     3181 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     3241 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     3301 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     3361 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     3421 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     3481 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc
     3541 tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg
     3601 tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc
     3661 tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga
     3721 tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg
     3781 tccgggggat gtcggggccg cgacactcgc tgcagagctt gccgccgccg caggtggagc
     3841 tgacttcatc cgcacacacg agccgcgccc cttgcgcgac gggctggcgg tattggcggc
     3901 gctgaaagaa accgcaagaa ttcgttaact gcacattcgg gatatttctc tatattcgcg
     3961 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
     4021 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
     4081 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
     4141 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg
     4201 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
     4261 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
     4321 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
     4381 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
     4441 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
     4501 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
     4561 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
     4621 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
     4681 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
     4741 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
     4801 ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt
     4861 tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg
     4921 aaacctataa aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat
     4981 gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc
     5041 ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt
     5101 gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt
     5161 ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca
     5221 ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc
     5281 tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc
     5341 ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat
     5401 atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag
     5461 atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg
     5521 tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat
     5581 ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac
     5641 ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc caaagcctta
     5701 gcgtaggatt ccgccccttc ccgcaaacga ccccaaacag gaaacgcagc tgaaacggga
     5761 agctcaacac ccactgacgc atgggttgtt caggcagtac ttcatcaacc agcaaggcgg
     5821 cactttcggc catccgccgc gccccacagc tcgggcagaa accgcgacgc ttacagctga
     5881 aagcgaccag gggctcggcg tggcaagact cgcagcgaac ccgtagaagg ggtctgacgc
     5941 tcagtggaac gaaaactcac gttaagcaac gttttctgcc tctgacgcct cttttaatgg
     6001 tctcagatgt cctttggtca ccagttctgc cagcgtgaag gaataatggc cgagcatatt
     6061 gatatgtccg tggcaaagcg gggagaggcg tgcgatatct tcatcattca gtgtttcacc
     6121 ctgcgcccgg agatgatcca gggctgcctg catataaata gtgttccata acacgacggc
     6181 gttagtgacc agccccagtg tgcccagttg atcttcctga ccgtcggtat atcgttttct
     6241 tatctcacct ttttgaccgt gacagatggc tctggcaacg gcatggcgac tttctccccg
     6301 attaagctgg gtcagaatgc gccggcggta atcttcatca tcaatataat taagcagata
     6361 cagcgttttg ttgatgcgcc ccacttcaat gattgcctga gtcagtccgg aaggacgttc
     6421 acttttcagc aatgaacgga ccagcactga aacctgtact ttgcccagct tcagggagcc
     6481 agcggtccgg atcatttcgt cccactgaag gactattttt cggggatctg attgccctct
     6541 ggcaatatca ttcagcacgc catagtcggc atcatggtcc attcgccaga aaaccgaagc
     6601 accggcatca gccaggcgtg gagaaaactg gtatcccagc agccagaaaa ggccaaagac
     6661 aagttcgctg gcacctgctg tatcggtcat aatttcggtt ggattcagcc cggtctcctg
     6721 ttccagaaga ccttccagca caaagataga gtccctcagc gtccccggta taacgatgcc
     6781 atgaaagccg gaatactgat cggacacaaa gttgtaccag gtgatccctc tgttattacc
     6841 aaagtatttg cggttcggtc cggcattgat tgttctgact ggcgtaacaa agcgcattcc
     6901 atctgcagat gccacttctc ctccacccca tatctgtgcc agtggcagcg ttgcctgaaa
     6961 atcaaccagt ctggcattag cgctggtgat agtttcagcc cgcagatagt tcgcttttgt
     7021 ccagttcagc cggtgtcggg tcagtgcagg aacatttgat ctgatcagtg gttccagacc
     7081 gatattgcag gcttcagcca tcagcacggc gctgatgctg acgggcagat catcaactct
     7141 ggcactggct tcactagcat ggaaaaactc atcagcaaat ccggtatggg cgttaatttc
     7201 gagcagcaac tccgttaaat ccaccggagg gagtagatca ctgatcattt tgctcagtcg
     7261 tttcagactg tccggctcat caagactggc gaggggagaa attgtcaacc ggggcttcgg
     7321 gccagaaaca tcgagttcga cagcctcatt ttcgcaaaga cgtgcagcaa cctgtctgta
     7381 acgactatca agctgatgac ccagagattt tattgcttcc tgcgggtctg tcgggtgccc
     7441 caaagaacga taaaccttaa tccggtttgc ctgccagtca gcaccctgta gtaatcttgc
     7501 acgaggatct ccccaccggt tactgccggt aacgtagaca tccctccgcc tcagactatc
     7561 ctgcagttta ctgagaaagc agagcgtgta tcccctgcgg gtgatatgtt tttccttgtt
     7621 aatcaccagc cgtttccatg accgactgat aatttccgtt ggtgcgtcgt caaaaaactg
     7681 ccgccgtgag ctgaactccc ggctgaggta gtcacaggca ttcagagtgg taaccccggc
     7741 aggtgcggat gaaaatttaa cggtattcag cagatggggc aggaaacgac gaacgcgccc
     7801 gtactgctcc accatttctt catgaaaatt atcgtctgag ggccgggcaa tttcacggac
     7861 aagcgtgatg atttcagcca gcttttgcct tgggatgtag ctgaacacct cagcacgaat
     7921 cgattcgtcc ggtgtttctt ctttcagcag gtacgaacat gcgctggcga gcgccaatgc
     7981 agatttatcc agatccttca gcgagcggag ccgttttttc tgcccaatct ttctggcgtc
     8041 acggatgata acggccagca tggcgtccag aacgtccaat gcatcatcca gcgccagcgt
     8101 ttcccatgca aggacaaagg caaccagaac cgccatcctt ttctgcggtg acatcctggc
     8161 aatattgaac accgaagtca taccagcata acgtgcgaga tttttcaggc gcacagccgg
     8221 gagtgtactc aggttttcag catgcaggcc aaaatcgttc agagttttcc agcgttcaat
     8281 tgcttcatta aacgccggac cactgatggt cacagggccc tttttcagtg attccagtaa
     8341 agacaggcgg ctgcaatcag ttggccccag cagcatctcc agctgtgaac gctgttcggc
     8401 tgacggtatc agtgccagtt tgtggcactg ttgcaaagtt agcgatgagg cagccttttg
     8461 tct
//
LOCUS       DAZFKM010000702         6460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.guided.212, whole genome shotgun sequence.
ACCESSION   DAZFKM010000702 DAZFKM010000000
VERSION     DAZFKM010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.guided.212"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..619
                     /locus_tag="ACHKAY_006072"
     CDS             <1..619
                     /locus_tag="ACHKAY_006072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO5704457.1"
                     /translation="GQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            616..1230
                     /gene="prfH"
                     /locus_tag="ACHKAY_006073"
     CDS             616..1230
                     /gene="prfH"
                     /locus_tag="ACHKAY_006073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO5704458.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1287..2744)
                     /gene="pepD"
                     /locus_tag="ACHKAY_006074"
     CDS             complement(1287..2744)
                     /gene="pepD"
                     /locus_tag="ACHKAY_006074"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO5704459.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3005..3463
                     /gene="gpt"
                     /locus_tag="ACHKAY_006075"
     CDS             3005..3463
                     /gene="gpt"
                     /locus_tag="ACHKAY_006075"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO5704460.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3555..4799
                     /gene="frsA"
                     /locus_tag="ACHKAY_006076"
     CDS             3555..4799
                     /gene="frsA"
                     /locus_tag="ACHKAY_006076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO5704461.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4857..5258
                     /gene="crl"
                     /locus_tag="ACHKAY_006077"
     CDS             4857..5258
                     /gene="crl"
                     /locus_tag="ACHKAY_006077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO5704462.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5297..6352)
                     /gene="phoE"
                     /locus_tag="ACHKAY_006078"
     CDS             complement(5297..6352)
                     /gene="phoE"
                     /locus_tag="ACHKAY_006078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO5704463.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1577 a         1581 c         1660 g         1642 t
ORIGIN      
        1 ggggcagtct attttacagc ggcatattgc ctcgttctcg catcatggtt tgcctcaagg
       61 cagtgacgac gcgctacggt atattgcgga acatgacgat gcgctggcgt ttgcgtgtat
      121 taatcgccag atgatcgctt tgcgcataat gcaacaagtt aaggccacgg gaagtccggt
      181 tctggatgtg gcgcataact ttgttagcgc gtgtcaaatc ggcgatcaac agggctggtt
      241 gcatcgtaaa ggtgccacgc cggatgacaa cggtctggtg attattcccg gttcacgtgg
      301 tgattactcc tggattgtac agcctgttgc gaacgaaaaa acgctccact cgctggcgca
      361 tggggcaggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag cgaaatacac
      421 ggcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg ataaacaact
      481 catctttgaa gaagcgccac aagcctataa atcggctgaa agcgtggtgc aatgtctggt
      541 gcaggccggt ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag
      601 tggagggaaa aaaggatgat cttgctacaa ctctcctcag cgcaggggcc ggaagaatgt
      661 tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg acaagacgtc
      721 gcggtaacgg tgctggaaac agaaacgggc cactactctg acacgctgcg ttcggcgctg
      781 atttctctgg atggcgataa cgcatgggca ttaagcgaaa gctggtgtgg cactattcag
      841 tggatttgtc cgagtccgta tcggcctcat catgggcgca aaaactggtt tctgggcatt
      901 gggcgtttta ccgctgatga gcaggaacaa tcggatgcaa tccgttatga gacgttgcgt
      961 tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg cgccacgcat
     1021 ttggcatccg gtattagcgt gaaggttcag tcagagcgta gtcagcatgc taacaagcgg
     1081 ctggcacgat tgctgattgc ctggaagctg gagcagcagc aacaggaaaa tagcgcggtg
     1141 ctgaaatcgc tgcggcgaat gttccatcac cagattgaac gtggcaaccc gagacggaca
     1201 tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga aggcgattaa
     1261 acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag cagttcagtc
     1321 agcagcgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg agagtgtgga
     1381 ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt tttgaacaga
     1441 ccacattcca ggcccgcgtg gataatctgg atgttcggtg ttttgttgaa cagacgctga
     1501 taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca gccaggatat
     1561 gcgcctttcg cttcggtttt cgcgccagct agtttaccca gcgaatccag catgctcacc
     1621 acgtagtctt taccgctgtc gatcagtgaa cggatcaggc agtgaatttc tacgttattg
     1681 tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt tgccacgtcg
     1741 gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt atcgcgagat
     1801 ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc cagatttttc
     1861 tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag agatttcagg
     1921 acgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta
     1981 cgcagtgtgc cgccgttgaa atcgataagg cgcagatcca gttcttccgc atgacccgcc
     2041 aggaagcgca ccagtagttt gttggcatta cccaggccaa cgtggatttc accgccggag
     2101 tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc tggaaccgct
     2161 tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc catgtagatt
     2221 tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca gttgctctgt
     2281 aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac ttccagcggg
     2341 ccgtgaacca cgttttcgtc agccagaacc gccagcgcag acgccatacc aatgccgtta
     2401 tctgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata aggctggata
     2461 ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat atcgaggtgg
     2521 gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttt acgaatcagg
     2581 atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca accaacaatg
     2641 tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat tttggcaaaa
     2701 atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag tctccttgtc
     2761 gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg tcaacacaag
     2821 acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc
     2881 gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct
     2941 cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact
     3001 tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact
     3061 cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg
     3121 tctggtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     3181 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     3241 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgctgttgc
     3301 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     3361 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg aacagccgtg
     3421 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     3481 actgccgggc gttgttcttt ttaacttcag gcaggttaca atagtttcca gtaagtattc
     3541 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     3601 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     3661 agtcggctct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     3721 ggatctggcg cggtattgac ccacgtgaaa tcctcgacgt ccaggcacgt attgtgatga
     3781 gcgatgctga acgtaccgac gatgatttat acgatactgt gattggctac cgtggtggca
     3841 actggattta tgagtgggcc acccaggcga tggtgtggca acaaaaagcc tgtgcggaag
     3901 aagatccgca actcagtggt cgacactggc tgcatgcggc tacgttgtac aacattgccg
     3961 cctatcctca tctgaaagga gatgacctgg ccgagcaagc gcaggcttta tcaaaccgcg
     4021 cctatgaaga ggccgcccag cgtctgccgg gcacgatgcg gcagatggag tttaccgtac
     4081 ccggcggtgc gcccatcacc ggctttttgc atatgccgaa aggcgatggc ccgttcccga
     4141 cagtattaat gtgtggtggt ctggatgcga tgcagacgga ctattacagc ctgtatgaac
     4201 gttattttgc gccgcgcggc attgcgatgc tgactattga tatgccatcg gtgggctttt
     4261 cttcaaaatg gaagctcacc caggactcca gcctgttgca tcagcacgtc ttaaaggcgc
     4321 tgcctaacgt accgtgggtg gatcacactc gcgtcgcggc ctttggtttc cgtttcggcg
     4381 ctaacgttgc cgtgcgtctg gcatatcttg aatcgccgcg tctgaaagcg gttgcctgtc
     4441 ttggtccggt agttcatacc ctgttgagtg attttaagtg ccagcaacag gtgccggaaa
     4501 tgtatcttga cgttctggcg agtcgtttgg ggatgcatga tgcttccgat gacgcgttac
     4561 gcgtggagct gaatcgctat tcattaaaag tgcaaggatt gctggggcgt cgctgcccaa
     4621 cgccaatgtt atcaggctac tggaagaacg atccgttcag cccggaagag gactcacgct
     4681 taatcacctc atcatctgct gacggtaaat tattagagat cccatttaac ccggtgtatc
     4741 ggaattttga caaaggtctt caggaaatca ccggctggat cgaaaaacgc ttgtgttaaa
     4801 aatttgctaa attttgccaa tttggtaaaa cagttgcatc acaacaggag atagcaatga
     4861 cgttaccgag tggacacccg aagagcagat tgatcaaaaa atttaccgca ctaggcccgt
     4921 atattcgtga aggtaagtgc gaagataatc gattcttttt cgattgtctg gctgtatgcg
     4981 tcaacgtgaa accggcaccg gaagtgcgtg agttctgggg ctggtggatg gagcttgaag
     5041 cgcaggaatc ccgttttacc tacagttacc agtttggtct gttcgataaa gcaggcgact
     5101 ggaagagtgt tccggtaaaa gacactgaag tggttgaacg actggagcac accctgcgtg
     5161 agtttcacga gaagctgcgt gaactgctga cgacgctgaa tctgaagctg gaaccggcgg
     5221 atgattttcg tgacgagccg gtgaagttaa cggcgtgagt gaaatgtgcc ggatgcatca
     5281 catccggcaa taatcattaa aactgatagg tcatgccaac cgcgacaata tcatcattat
     5341 taatattcaa tttgttatcg ctatccagtt ggttgatttt ataatcaaca aacgctgaca
     5401 tatttttgtt gaaataatat gtagccccga catcgatata attgaccaga tcttcatcac
     5461 cgataccttc aatatctttc cctttcgata agacataacc cagcgatgga cgcagaccaa
     5521 agtcaaactg gtattgagcg accgcttcaa agttctgtgt cttattggca aagccgccag
     5581 ttattggcgt cattttgcgt gtttcagaat aaaaagttgc cagataaaaa ttattggcat
     5641 cgtatttcag acctgtagcc catgcttctg cacgcttgcc tgtgccacgg ctttgcaggt
     5701 tctgctcgtt ggtgcgatct gagttggtat aggccccact aatggcgaaa tcgctgccgc
     5761 caaagtcata tgtcaatgac gtgccgaagc catcgccgtt ttgcttttta acgtcgcggt
     5821 tttcgttttt cccttgatat tgcagggtta agttcaggcc atcgataacg ccgaagaagt
     5881 cggtgttccg atacgtcgcc agaccgctgg cgcgtttggt cataaagttg tcggtctgcg
     5941 ccgaggaatc gccaccaaat tccgggaaca tatcggtcca ggcttccacg tcatacaacg
     6001 cgcccaggtt acgaccataa tcgaaagaac ccaaatcttt atatttcaac ccggcaaaag
     6061 cgagacgcgt tttttgctgt gcggtatcac tctccgcttt atttccggca aactccgctt
     6121 cccaccgacc ataaccagtc agttgatcgt taatttgtgt ttcaccttta aaaccaaaac
     6181 ggatataact ctggtcgcca tctttactgg cgttatcact catataatgc atggctttaa
     6241 ctttgccata gacatccagt ttattaccgt ctttattata tatttctgca gcctgtacag
     6301 atgcagatgc cacaatgccc atcaccacta atgccagagt gctctttttc attttcattc
     6361 ctgattttaa ttaacgcgcg aatattcagc gggagagccc cgttgaaaac aggaaagttt
     6421 ttaacctgag attgttaaag atatattaca gattaataat
//
LOCUS       DAZFKM010000703         1498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.guided.376, whole genome shotgun sequence.
ACCESSION   DAZFKM010000703 DAZFKM010000000
VERSION     DAZFKM010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.guided.376"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..971
                     /gene="stxA1a"
                     /locus_tag="ACHKAY_006079"
     CDS             24..971
                     /gene="stxA1a"
                     /locus_tag="ACHKAY_006079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO5704464.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGSGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            981..1250
                     /gene="stxB1a"
                     /locus_tag="ACHKAY_006080"
     CDS             981..1250
                     /gene="stxB1a"
                     /locus_tag="ACHKAY_006080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO5704465.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          407 a          256 c          374 g          461 t
ORIGIN      
        1 gtgctcaagg agtattgtgt aatatgaaaa taattatttt tagagtgcta acttttttct
       61 ttgttatctt ttcagttaat gtggtggcga aggaatttac cttagacttc tcgactgcaa
      121 agacgtatgt agattcgctg aatgtcattc gctctgcaat aggtactcca ttacagacta
      181 tttcatcagg aggtacgtct ttactgatga ttgatagtgg ctcaggggat aatttgtttg
      241 cagttgatgt cagagggata gatccagagg aagggcggtt taataatcta cggcttattg
      301 ttgaacgaaa taatttatat gtgacaggat ttgttaacag gacaaataat gttttttatc
      361 gctttgctga tttttcacat gttacctttc caggtacaac agcggttaca ttgtctggtg
      421 acagtagcta taccacgtta cagcgtgttg cagggatcag tcgtacgggg atgcagataa
      481 atcgccattc gttgactact tcttatctgg atttaatgtc gcatagtgga acctcactga
      541 cgcagtctgt ggcaagagcg atgttacggt ttgttactgt gacagctgaa gctttacgtt
      601 ttcggcaaat acagagggga tttcgtacaa cactggatga tctcagtggg cgttcttatg
      661 taatgactgc tgaagatgtt gatcttacat tgaactgggg aaggttgagt agcgtcctgc
      721 ctgactatca tggacaagac tctgttcgtg taggaagaat ttcttttgga agcattaatg
      781 caattctggg aagcgtggca ttaatactga attgtcatca tcatgcatcg cgagttgcca
      841 gaatggcatc tgatgagttt ccttctatgt gtccggcaga tggaagagtc cgtgggatta
      901 cgcacaataa aatattgtgg gattcatcca ctctgggggc aattctgatg cgcagaacta
      961 ttagcagttg agggggtaaa atgaaaaaaa cattattaat agctgcatcg ctttcatttt
     1021 tttcagcaag tgcgctggcg acgcctgatt gtgtaactgg aaaggtggag tatacaaaat
     1081 ataatgatga cgataccttt acagttaaag tgggtgataa agaattattt accaacagat
     1141 ggaatcttca gtctcttctt ctcagtgcgc aaattacggg gatgactgta accattaaaa
     1201 ctaatgcctg tcataatgga gggggattca gcgaagttat ttttcgttga ctcagaatag
     1261 ctcagtgaaa atagcaggcg gagattcata aatgttaaat acatctcaat tcagtcagtt
     1321 gttgccggtc tgataataga tgtgttagaa aatttctgca tggtgaatcc ccctgtgcgg
     1381 aggggcgact ggtgaacggt atgatctctt tgatgatcgt aagcgagaat acgcgggttt
     1441 ggtggcacca ggccgaactc accgggaggc acccggcatc atgctgtata cagagatt
//
LOCUS       DAZFKM010000704         1258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.guided.414, whole genome shotgun sequence.
ACCESSION   DAZFKM010000704 DAZFKM010000000
VERSION     DAZFKM010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.guided.414"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..593
                     /gene="dfrA8"
                     /locus_tag="ACHKAY_006081"
     CDS             84..593
                     /gene="dfrA8"
                     /locus_tag="ACHKAY_006081"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HFO5704466.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            590..1246
                     /locus_tag="ACHKAY_006082"
     CDS             590..1246
                     /locus_tag="ACHKAY_006082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFO5704467.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAHVKDAIGA
                     SVASKWTAIIHAPVNGVVLGIPDHGFDGKELASYGFGLVLAGHYHNHKKIGTVANSRW
                     "
BASE COUNT          309 a          332 c          322 g          295 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc cgacgcaata atcaccaatt
       61 cctcttttga taggtaaaca acaatgatcg agcttcatgc cattttagct gccaccgcca
      121 atggttgcat tgggaaggac aacgcacttc cctggccacc actaaaaggc gatctggcca
      181 gattcaaaaa attgaccatg gggaaggtgg tcattatggg gcgcaagacc tatgagagct
      241 tgcccgtcaa attagaaggt cgcacctgca tcgttatgac gcgccaagcg ctggagcttc
      301 cgggtgttcg tgacgctaac ggcgctatct tcgtgaacaa cgtcagcgac gccatgcggt
      361 tcgctcaaga agagagcgtg ggcgatgtgg cctacgtcat tggtggcgct gagatattca
      421 agcgacttgc cttgatgatc acgcagattg aattgacctt tgttaagcga ctgtacgaag
      481 gcgacaccta cgttgatctg gccgaaatgg tcaaagacta cgagcagaat ggcatggaag
      541 aacatgacct tcacacttac ttcacttacc gtaaaaagga gcttacagaa tgaccttcgt
      601 tgccatttca gatacgcacc tgcacaactg gtcgcagttc gcgataccaa cagagtcggg
      661 cattaactcc aggctgttgc agattcttaa agccattgaa gaagcggctt gcgccgccga
      721 ttaccacgcc ccagcgggcg tggttccaac cgtctatcac ggcggcgatc tcttccatgt
      781 gcgaggttct ttgacccctt ctgtgctaaa cgcggtgctc gactttttta agaccatcca
      841 ccgtgactac ggcgttcgct ttcgcatgat cgccggcaat cacgatctgg aaaccaaaga
      901 ctcatgtcct atgggcaatg cagctgcggc gcttaattcg ttgccgtttg tggaggttgt
      961 atccgaaaaa acgctgttcg aggaccacaa agtcgctctg ctgccatggc gcgattccat
     1021 ggacgatctg cgtgcggatc ttgcgcacgt taaagacgcg atcggcgcta gtgttgcctc
     1081 taagtggaca gcaatcatcc acgcaccggt taacggggtt gttcttggca ttccagacca
     1141 tgggttcgac ggaaaagagc tcgcctccta tggcttcggg ttagtgctcg ccggtcacta
     1201 ccacaaccac aaaaaaatcg gcactgttgc aaatagtcgg tggtgataaa cttatcat
//
LOCUS       DAZFKM010000705         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1810F67649
            SAMN43251691-rid23065673.guided.420, whole genome shotgun sequence.
ACCESSION   DAZFKM010000705 DAZFKM010000000
VERSION     DAZFKM010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251691
            Sequence Read Archive: SRR30293367
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350192.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 01:41:38
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 6,083
            CDSs (total)                      :: 5,979
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 104
            rRNAs                             :: 2, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 111 of 268
            Pseudo Genes (incomplete)         :: 152 of 268
            Pseudo Genes (internal stop)      :: 66 of 268
            Pseudo Genes (multiple problems)  :: 52 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251691-rid23065673.guided.420"
                     /serotype="O 26:H11"
                     /isolate="1810F67649"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            268..1128
                     /locus_tag="ACHKAY_006083"
     CDS             268..1128
                     /locus_tag="ACHKAY_006083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO5704468.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          340 a          266 c          287 g          340 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc cgctccacgg tttataaaat
       61 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      121 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctggtaaatg
      241 cttcaataat attgaaaaag gaagagtatg agtattcaac attttcgtgt cgcccttatt
      301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      481 gttctgctat gtggtgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
      541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      661 gctgccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      781 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
      841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1201 gtgaagatcc tttttgataa tctcatgacc aaa
//
